KEGG   ENZYME: 1.14.14.174
Entry
EC 1.14.14.174              Enzyme                                 

Name
geranylhydroquinone 3''-hydroxylase;
GHQ 3''-hydroxylase;
CYP76B74 (gene name);
geranylhydroquinone,NADPH:oxygen oxidoreductase (3''-hydroxylating)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
geranylhydroquinone,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (3''-hydroxylating)
Reaction(IUBMB)
geranylhydroquinone + [reduced NADPH---hemoprotein reductase] + O2 = 3''-hydroxygeranylhydroquinone + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R09042]
Reaction(KEGG)
R09042
Substrate
geranylhydroquinone [CPD:C10793];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
3''-hydroxygeranylhydroquinone [CPD:C18132];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein found in plants, where it is part of the biosynthesis pathway of the red naphthoquinone pigment shikonin.
History
EC 1.14.14.174 created 2010 as EC 1.14.13.116, transferred 2020 to EC 1.14.14.174
Pathway
ec00130  Ubiquinone and other terpenoid-quinone biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K24441  geranylhydroquinone 3''-hydroxylase
Reference
1  [PMID:10664138]
  Authors
Yamamoto H, Inoue K, Li SM, Heide L.
  Title
Geranylhydroquinone 3"-hydroxylase, a cytochrome P-450 monooxygenase from Lithospermum erythrorhizon cell suspension cultures.
  Journal
Planta 210:312-7 (2000)
DOI:10.1007/PL00008139
Reference
2  [PMID:30498024]
  Authors
Wang S, Wang R, Liu T, Lv C, Liang J, Kang C, Zhou L, Guo J, Cui G, Zhang Y, Werck-Reichhart D, Guo L, Huang L
  Title
CYP76B74 Catalyzes the 3''-Hydroxylation of Geranylhydroquinone in Shikonin Biosynthesis.
  Journal
Plant Physiol 179:402-414 (2019)
DOI:10.1104/pp.18.01056
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.174
IUBMB Enzyme Nomenclature: 1.14.14.174
ExPASy - ENZYME nomenclature database: 1.14.14.174
BRENDA, the Enzyme Database: 1.14.14.174

DBGET integrated database retrieval system