KEGG   ENZYME: 1.3.98.4Help
Entry
EC 1.3.98.4                 Enzyme                                 

Name
5a,11a-dehydrotetracycline reductase;
oxyR (gene name);
12-dehydrotetracycline dehydrogenase;
dehydrooxytetracycline dehydrogenase;
12-dehydrotetracycline reductase
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With other, known, physiological acceptors
BRITE hierarchy
Sysname
tetracycline:coenzyme F420 dehydrogenase
Reaction(IUBMB)
tetracycline + oxidized coenzyme F420 = 5a,11a-dehydrotetracycline + reduced coenzyme F420 [RN:R05459]
Reaction(KEGG)
R05459;
(other) R09192
Show
Substrate
tetracycline [CPD:C06570];
oxidized coenzyme F420 [CPD:C00876]
Product
5a,11a-dehydrotetracycline [CPD:C03206];
reduced coenzyme F420 [CPD:C01080]
Comment
The enzyme, characterized from the bacteria Streptomyces aureofaciens and Streptomyces rimosus, catalyses the last step in the biosynthesis of the tetracycline antibiotics tetracycline and oxytetracycline.
History
EC 1.3.98.4 created 2016
Pathway
ec00253  Tetracycline biosynthesis
ec01057  Biosynthesis of type II polyketide products
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
ec01130  Biosynthesis of antibiotics
Orthology
K21301  5a,11a-dehydrotetracycline reductase
Genes
KAU: B6264_18540
Taxonomy
Reference
1
  Authors
McCormick, J.R.D., Hirsch, U., Sjolander, N.O. and Doerschuk, A.P.
  Title
Cosynthesis of tetracyclines by pairs of Streptomyces aureofaciens mutants.
  Journal
J Am Chem Soc 82:5006-5007 (1960)
Reference
2
  Authors
Miller, P.A., Sjolander, N.O., Nalesnyk, S., Arnold, N., Johnson, S., Doerschuk, A.P. and McCormick, J.R.D.
  Title
Cosynthetic factor I, a factor involved in hydrogen-transfer in Streptomyces aureofaciens.
  Journal
J Am Chem Soc 82:5002-5003 (1960)
Reference
3
  Authors
McCormick, J.R.D. and Morton, G.O.
  Title
Identity of cosynthetic factor I of Streptomyces aureofaciens and fragment FO from coenzyme F420 of Methanobacterium species.
  Journal
J Am Chem Soc 104:4014-4015 (1982)
Reference
4  [PMID:23621493]
  Authors
Wang P, Bashiri G, Gao X, Sawaya MR, Tang Y
  Title
Uncovering the enzymes that catalyze the final steps in oxytetracycline biosynthesis.
  Journal
J Am Chem Soc 135:7138-41 (2013)
DOI:10.1021/ja403516u
Other DBs
ExplorEnz - The Enzyme Database: 1.3.98.4
IUBMB Enzyme Nomenclature: 1.3.98.4
ExPASy - ENZYME nomenclature database: 1.3.98.4
BRENDA, the Enzyme Database: 1.3.98.4

DBGET integrated database retrieval system