KEGG ENZYME: 2.3.1.65

ENZYME: 2.3.1.65

Entry

EC 2.3.1.65                 Enzyme

Name

bile acid-CoA:amino acid N-acyltransferase;
glycine---taurine N-acyltransferase;
amino acid N-choloyltransferase;
BAT;
glycine N-choloyltransferase;
BACAT;
cholyl-CoA glycine-taurine N-acyltransferase;
cholyl-CoA:taurine N-acyltransferase

Class

Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups

Sysname

choloyl-CoA:glycine N-choloyltransferase

Reaction(IUBMB)

choloyl-CoA + glycine = CoA + glycocholate [RN:R03718]

Reaction(KEGG)

R03718;
(other) R03720 R08744 R08745

Substrate

choloyl-CoA [CPD:C01794];
glycine [CPD:C00037]

Product

CoA [CPD:C00010];
glycocholate [CPD:C01921]

Comment

Also acts on CoA derivatives of other bile acids. Taurine and 2-fluoro-beta-alanine can act as substrates, but more slowly [4]. The enzyme can also conjugate fatty acids to glycine and can act as a very-long-chain acyl-CoA thioesterase [7]. Bile-acid---amino-acid conjugates serve as detergents in the gastrointestinal tract, solubilizing long chain fatty acids, mono- and diglycerides, fat-soluble vitamins and cholesterol [4]. This is the second enzyme in a two-step process leading to the conjugation of bile acids with amino acids; the first step is the conversion of bile acids into their acyl-CoA thioesters, which is catalysed by EC 6.2.1.7, cholate---CoA ligase.

History

EC 2.3.1.65 created 1983, modified 2005

Pathway

ec00120

Primary bile acid biosynthesis

ec00430

Taurine and hypotaurine metabolism

ec01100

Metabolic pathways

Orthology

K00659

bile acid-CoA:amino acid N-acyltransferase

Genes

HSA:

570(BAAT)

PTR:	472996 472997(BAAT)

PPS:	100992429(BAAT)

GGO:	101140183(BAAT)

PON:	100431907(BAAT) 100461881

PPYG:

129044183(BAAT) 129044494

NLE:	100598200(BAAT)

HMH:	116475340(BAAT)

SSYN:

129479097(BAAT)

MCC:	100429419(BAAT)

MCF:	102132073(BAAT)

MTHB:

126937773

MNI:	105480981(BAAT)

CSAB:

103219118(BAAT)

CATY:

105593023(BAAT)

PANU:

101007875(BAAT)

TGE:	112608227(BAAT)

MLEU:

105546769(BAAT) 105546778

RRO:	104670797(BAAT)

RBB:	108537176(BAAT)

TFN:	117098906(BAAT)

PTEH:

111536312(BAAT)

CANG:

105518150(BAAT)

CJC:	100394190 100396712(BAAT)

SBQ:

101036806

CIMI:

108303855(BAAT)

ANAN:

105727317 105727329

CSYR:

103256776 103256777

MMUR:

105873759(BAAT) 105873760

LCAT:

123645821(BAAT)

PCOQ:

105805378(BAAT) 105805407

OGA:	100957082 100957390

MMU:	12012(Baat) 209186(Acnat2) 230161(Acnat1)

MCAL:

110292343 110293184

MPAH:

110323775(Baat) 110323900

RNO:	29725(Baat) 313220(Acnat2) 681043(Acnat1)

MCOC:

116095310(Baat) 116096003

ANU:	117708778 117709136 117709479 117709552

MUN:	110557337 110557338

CGE:	100756252(Baat) 100756541

MAUA:

101835789 101836051

PROB:

127224676 127224678

PLEU:

114702315(Baat) 114702326

MORG:

121451765 121452021

MFOT:

126509815 126509848

AAMP:

119826151 119826854

NGI:	103749095 103749096 103749097

HGL:	101716142(Baat) 101717293

CPOC:

100719696(Baat) 100719973

CCAN:

109696243(Baat)

DORD:

105990724

DSP:	122122419(Baat) 122123761

PLOP:

125346048 125356113

NCAR:

124964800 124964957

MMMA:

107147751(Baat) 107147755

OCU:	100348617 100350133 100350872

OPI:	101519913(BAAT)

TUP:	102472797 102473647(BAAT)

GVR:

103607004

CFA:	481634 481635(BAAT)

CLUD:

112649971(BAAT) 112649972

VVP:	112930766(BAAT) 112930863

VLG:	121494953 121494954

NPO:	129494523 129494875

AML:	100476252 100476755

UMR:	103678524 103678630(BAAT)

UAH:	113260385 113260386

UAR:	123781224 123781225

ELK:	111145724 111145726

LLV:	125082990 125083316

MPUF:

101671389 101671902(BAAT)

MNP:	132024553 132024555

MLK:	131812402 131812403

NVS:	122917401 122917402

ORO:	101365672 101365978

EJU:	114213433 114213445

ZCA:

113934001

MLX:	118018214(BAAT) 118018215

NSU:	110581717(BAAT) 110581808

LWW:	102729180 102729479

FCA:	101095691(BAAT) 101096116

PYU:	121034756(BAAT) 121034758

PCOO:

112850975 112860692(BAAT)

PBG:	122474312 122474485(BAAT)

PVIV:

125150632 125150645

LRUF:

124526231 124526233

PTG:	102969591(BAAT) 102969870

PPAD:

109270057 109270058

PUC:	125926097 125926223

AJU:	106972069 106972070

HHV:	120224623(BAAT) 120224624

BTA:	530653 786798

BOM:

102268462

BIU:	109563300(BAAT)

BBUB:

102403928 102405119

BBIS:

104992074

CHX:	102174570(BAAT)

OAS:	101106149 101106409(BAAT)

BTAX:

128052871

ODA:

120863959

CCAD:

122431993

SSC:	100515185 102158534 110255172

CFR:	102523415(BAAT)

CBAI:

105067405(BAAT)

CDK:	105086911(BAAT)

VPC:	102529299(BAAT)

BACU:

103006095 103017253(BAAT)

BMUS:

118896620 118897292(BAAT)

LVE:	103081315 103082496(BAAT)

OOR:	101284164 101284403

DLE:	111188015(BAAT)

PCAD:

102984994(BAAT)

PSIU:

116754977 116755602

NASI:

112392644(BAAT) 112392655

ECB:	100054567 100063137(BAAT)

EPZ:	103552469(BAAT) 103568036

EAI:	106831794 106831795

MYB:	102262596 102263115

MYD:	102764048 102764710

MMYO:

118667244 118667245

MLF:	102433860 102434162(BAAT)

MDT:	132212209 132212211

PKL:	118716241 118716242

EFUS:

103286385(BAAT)

MNA:	107546604(BAAT)

DRO:	112304453(BAAT)

SHON:

118979701(BAAT)

AJM:	119045945(BAAT)

PDIC:

114503289(BAAT)

PHAS:

123819950(BAAT)

MMF:	118629687(BAAT)

PPAM:

129079385(BAAT)

HAI:	109395040(BAAT)

RFQ:	117032410(BAAT)

PALE:

102892341(BAAT) 102897591

PGIG:

120597018

PVP:	105296259(BAAT) 105296291

RAY:	107499578 107499579

MJV:	108397381(BAAT)

TOD:	119232402(BAAT)

SARA:

101540428(BAAT)

SETR:

126003761 126003845(BAAT)

LAV:	100654296 100670223

TMU:

101357199

ETF:	101654639 101654956

DNM:	101418518 101419268 101447075

MDO:	100017693 100023136(BAAT)

GAS:	123230833(BAAT) 123230834 123254112

SHR:	100924464 100925244

AFZ:

127554786

PCW:	110219134(BAAT)

TVP:	118831131 118832375

OAA:	100084056(BAAT)

GGA:	769879(BAAT)

PCOC:

116242919

MGP:

100539658

CJO:

107314177

TPAI:

128085669

LMUT:

125694885(BAAT)

NMEL:

110400838

APLA:

101797844

ACYG:

106048282

CATA:

118244478

AFUL:

116490259

TGU:

115495423

LSR:

110485078

SCAN:

103822142

PMOA:

120499625

OTC:

121339708

PRUF:

121355202

GFR:

102037789

FAB:

101815920

OMA:

130254435

PHI:

102101793

PMAJ:

107206148

CCAE:

111930612

CCW:

120410054

CBRC:

103611827

ETL:

114063343

ZAB:

102062391

ZLE:

135448205

ACHL:

103805502(BAAT)

SVG:

106854675

MMEA:

130580050

HRT:

120753731

SATI:

136362116

FPG:

101924136

FCH:

102059453

NNT:

104406607

SHAB:

115606966

ACUN:

113481099

TALA:

116961616

ACHC:

115352108

HLE:	104829793(BAAT)

AGEN:

126046987

GCL:

127017185

MNB:	103778534(BAAT)

DPUB:

104296264(BAAT)

EGZ:	104134405(BAAT)

NNI:	104008979(BAAT)

PCRI:

104030139

AFOR:

103895438

CLV:

102094683

CUCA:

104063337

TEO:

104377729

OHA:

104328517

ACAR:

104522067

CPEA:

104393367

CVF:	104283272(BAAT)

RTD:

128908803

AAM:

106494986

AROW:

112960829(BAAT)

NPD:

112952722

TGT:

104578818

DNE:

112988642

SCAM:

104151146(BAAT)

ASN:	102368085(BAAT) 102376159 102383708

AMJ:	102559248 102564970(BAAT) 102575228

CPOO:

109323479 109323497

GGN:	109297368 109297370

PSS:	102447700(BAAT) 102461463 102461871

CMY:	102939533(BAAT) 102947935

CCAY:

125636624(BAAT) 125638328

DCC:	119856403(BAAT) 119857880

CPIC:

101932563 101938826(BAAT)

TST:	117876003 117879669(BAAT)

CABI:

116825950(BAAT)

MRV:	120403019 120407254(BAAT) 120407813

ACS:	100566890(baat)

ASAO:

132772260(BAAT)

PVT:	110078385(BAAT)

SUND:

121930998

PBI:	103065567(BAAT)

PMUR:

107292645 107292672

CTIG:

120308320 120308321 120308323

TSR:	106538123(BAAT)

PGUT:

117667061(BAAT)

APRI:

131184117(BAAT)

PTEX:

113449132(BAAT)

NSS:	113421915(BAAT)

VKO:	123021162 123021248

PMUA:

114600844(BAAT)

PRAF:

128418018(BAAT)

ZVI:	118089420(BAAT)

HCG:	128341173 128341626(BAAT)

GJA:	107106123 107106126(BAAT)

STOW:

125427301 125436721(BAAT)

EMC:	129324679 129334736(BAAT)

XLA:	100137716(XB5872576.L) 108707337(baat.S) 121393106

XTR:	100125079(baat) 100488333 100496940 100497260 100497423 100497580 100497738 116408197

NPR:	108790690 108790705 108790706 108795509

RTEM:

120926637 120926656 120926672 120943647

BBUF:

120992241(BAAT) 121008572

BGAR:

122924848 122924849 122924850 122945314

MUO:	115482004(BAAT)

SRX:

107726829

SANH:

107689220 107689257

CCAR:

109068937

CAUA:

113061125 113068447

IPU:

108280978

IFU:

128625280

PHYP:

113537864

TFD:

113656460

TVC:

132838449

TRN:

134301802

CMAO:

118799894

CHAR:

105912192

TFS:

130523915

TNG:	GSTEN00014025G001 GSTEN00019694G001

NCC:

104954762

TBEN:

117489722

CGOB:

115008157

PGEO:

117447318

GACU:

117534190

EMAC:

134882281

ELY:	117256189 117256459 117256968

EFO:	125888830 125897968 125897977 125897983 125897996

PLEP:

121953471 121953523

SLUC:

116066678

ECRA:

117944007

ESP:

116688512

PFLV:

114553794

GAT:

120835251

PPUG:

119193948

AFB:

129105536

CLUM:

117730964

PSWI:

130208996

SCHU:

122867513

CUD:

121506802

ALAT:

119021228

ONL:

109198392

OAU:

116329896

OLA:

101166298

OML:

112145720

CSAI:

133448682

XMA:

102226402

XCO:

114135870

XHE:

116711136

PRET:

103465285

PFOR:

103144302

PLAI:

106934498

PMEI:

106906822

GAF:

122834742

PPRL:

129353154

CVG:

107083302

CTUL:

119791826

GMU:

124856113

NFU:

107385610

KMR:

108235667

NWH:

119411976

MCEP:

125006140

POV:	109628957 109644008

SSEN:

122767935 122768787

HHIP:

117761208

HSP:

118105133

PPLT:

128459351

SMAU:

118309812

SDU:

111221490

SLAL:

111667779

HCQ:

109522368

SSCV:

125976910

SBIA:

133507447 133507554

PEE:

133409198

PTAO:

133483319

BPEC:

110161506 110171280 110171322

MALB:

109956248

BSPL:

114856054

SJO:

128356051

SASA:

106584567 106609489

STRU:

115208005

OGO:

124003938

ONE:

115143032

OKI:

109894173

OKE:

118388720

SNH:	120062369 120065229

CCLU:

121577792

ELS:

105010081

SFM:

108933858

AANG:

118211819

LCM:	102356569(BAAT) 102363365 102363627

CIN:	100175018 100182847 100184394

SCLV:

120325725 120329571 120329639 120337556 120343818 120343819 120344834 120347055 120348310

SPU:	100892177 115926355

APLC:

110981446 110989383 110991145

ARUN:

117295575 117296628 117297972 117299118

AJC:	117105456 117105473

HAME:

121871783

PCLA:

123754342

PTRU:

123506716

HAZT:

108682704

LGI:	LOTGIDRAFT_125734

PVUL:

126821684

PCAN:

112570277 112570289 112570596

HRF:	124137037 124146464

HRJ:	124268350 124270995 124270996 124270998 124276134 124277115 124277116

CRG:

105328098

CANU:

128163038 128175585

CVN:	111131209 111131381

OED:	125652746 125652747

MYI:

110465077

PMAX:

117323580 117335375 117335376 117336519

MMER:

123550893 123552208

RPHI:

132733646 132733647 132737676

DPOL:

127853306 127853745 127870784 127873764

MAEA:

128209818 128225590

OBI:

106881808

OSN:	115226141 115226175 115232312 115232313

LJP:

135480621

LAK:

106169569

NVE:

5519541

EPA:	110233238 110233246 110242348

ADF:

107346883

AMIL:

114966874

PDAM:

113665012 113665467 113666427

SPIS:

111328316 111331670 111339322 111339330 111339338 111341936

HSY:	130624222 130648508 130662424

RES:	135680732 135680733 135684962 135696659 135696660 135696661 135696663 135696664 135696665 135696678 135696824

» show all

Reference

1 [PMID:7372637]

Authors

Czuba B, Vessey DA.

Title

Kinetic characterization of cholyl-CoA glycine-taurine N-acyltransferase from bovine liver.

Journal

J Biol Chem 255:5296-9 (1980)

Reference

Authors

Jordan TW, Lee R, Lim WC.

Title

Isoelectric focussing of soluble and particulate benzoyl-CoA and cholyl-CoA:amino acid N-acyltransferases from rat liver.

Journal

Biochem Int 1:325-330 (1980)

Reference

3 [PMID:422567]

Authors

Vessey DA.

Title

The co-purification and common identity of cholyl CoA:glycine- and cholyl CoA:taurine-N-acyltransferase activities from bovine liver.

Journal

J Biol Chem 254:2059-63 (1979)

Reference

4 [PMID:2037576]

Authors

Johnson MR, Barnes S, Kwakye JB, Diasio RB.

Title

Purification and characterization of bile acid-CoA:amino acid N-acyltransferase from human liver.

Journal

J Biol Chem 266:10227-33 (1991)

Sequence

[hsa:570]

Reference

5 [PMID:12454267]

Authors

Falany CN, Xie X, Wheeler JB, Wang J, Smith M, He D, Barnes S.

Title

Molecular cloning and expression of rat liver bile acid CoA ligase.

Journal

J Lipid Res 43:2062-71 (2002)
DOI:10.1194/jlr.m200260-jlr200

Reference

6 [PMID:12951368]

Authors

He D, Barnes S, Falany CN.

Title

Rat liver bile acid CoA:amino acid N-acyltransferase: expression, characterization, and peroxisomal localization.

Journal

J Lipid Res 44:2242-9 (2003)
DOI:10.1194/jlr.M300128-JLR200

Sequence

[rno:29725]

Reference

7 [PMID:12810727]

Authors

O'Byrne J, Hunt MC, Rai DK, Saeki M, Alexson SE.

Title

The human bile acid-CoA:amino acid N-acyltransferase functions in the conjugation of fatty acids to glycine.

Journal

J Biol Chem 278:34237-44 (2003)
DOI:10.1074/jbc.M300987200

Sequence

[hsa:570]

Other DBs

ExplorEnz - The Enzyme Database:

2.3.1.65

IUBMB Enzyme Nomenclature:

2.3.1.65

ExPASy - ENZYME nomenclature database:

2.3.1.65

BRENDA, the Enzyme Database:

2.3.1.65

CAS:	65979-40-0

DBGET integrated database retrieval system