KEGG   ENZYME: 2.4.1.65Help
Entry
EC 2.4.1.65                 Enzyme                                 

Name
3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase;
(Lea)-dependent (alpha-3/4)-fucosyltransferase;
alpha(1,3/1,4) fucosyltransferase III;
alpha-(1->4)-L-fucosyltransferase;
alpha-4-L-fucosyltransferase;
beta-acetylglucosaminylsaccharide fucosyltransferase;
FucT-II;
Lewis alpha-(1->3/4)-fucosyltransferase;
Lewis blood group alpha-(1->3/4)-fucosyltransferase;
Lewis(Le) blood group gene-dependent alpha-(1->3/4)-L-fucosyltransferase;
blood group Lewis alpha-4-fucosyltransferase;
blood-group substance Lea-dependent fucosyltransferase;
guanosine diphosphofucose-beta-acetylglucosaminylsaccharide 4-alpha-L-fucosyltransferase;
guanosine diphosphofucose-glycoprotein 4-alpha-L-fucosyltransferase;
guanosine diphosphofucose-glycoprotein 4-alpha-fucosyltransferase;
3-alpha-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase;
GDP-beta-L-fucose:3-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R 4I-alpha-L-fucosyltransferase;
GDP-L-fucose:3-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R 4I-alpha-L-fucosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
GDP-beta-L-fucose:beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R 4I-alpha-L-fucosyltransferase (configuration-inverting)
Reaction(IUBMB)
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R = GDP + beta-D-galactosyl-(1->3)-[alpha-L-fucosyl-(1->4)]-N-acetyl-beta-D-glucosaminyl-R [RN:R04553]
Reaction(KEGG)
R04553 > R06155(G);
(other) R06162(G) R06163(G) R06164(G) R06165(G)
Show
Substrate
GDP-beta-L-fucose [CPD:C00325];
beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R [CPD:C04656]
Product
GDP [CPD:C00035];
beta-D-galactosyl-(1->3)-[alpha-L-fucosyl-(1->4)]-N-acetyl-beta-D-glucosaminyl-R [CPD:C04850]
Comment
This enzyme is the product of the Lewis blood group gene. Normally acts on a glycoconjugate where R (see reaction) is a glycoprotein or glycolipid. Although it is a 4-fucosyltransferase, it has a persistent 3-fucosyltransferase activity towards the glucose residue in free lactose. This enzyme fucosylates on O-4 of an N-acetylglucosamine that carries a galactosyl group on O-3, unlike EC 2.4.1.152, 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase, which fucosylates on O-3 of an N-acetylglucosamine that carries a galactosyl group on O-4. Enzymes catalysing the 4-alpha-fucosylation of the GlcNAc in beta-D-Gal-(1->3)-beta-GlcNAc sequences (with some activity also as 3-alpha-fucosyltransferases) are present in plants, where the function in vivo is the modification of N-glycans. In addition, the fucTa gene of Helicobacter strain UA948 encodes a fucosyltransferase with both 3-alpha- and 4-alpha-fucosyltransferase activities.
History
EC 2.4.1.65 created 1972, modified 2001, modified twice 2002
Pathway
ec00513  Various types of N-glycan biosynthesis
ec00601  Glycosphingolipid biosynthesis - lacto and neolacto series
ec01100  Metabolic pathways
Orthology
K00716  galactoside 3(4)-fucosyltransferase 3
K07633  galactoside alpha-1,3-fucosyltransferase 5
K07634  galactoside alpha-1,3-fucosyltransferase 6
K14412  alpha-1,4-fucosyltransferase
K20151  alpha(1,3/1,4) fucosyltransferase
Genes
HSA: 2525(FUT3) 2527(FUT5) 2528(FUT6)
PTR: 455628(FUT5) 493975(FUT3) 493976(FUT6)
PPS: 100980873(FUT6) 100982106(FUT5) 100982431(FUT3)
GGO: 101153124(FUT5) 101153495(FUT6) 101154573(FUT3)
PON: 100431975(FUT6) 100432841 100433605(FUT5)
NLE: 100591958
MCC: 106994615(FUT5) 697582(FUT3)
MCF: 102144879(FUT5) 102145756
CSAB: 103233751(FUT5)
RRO: 104665387 104665388
RBB: 108526239
SBQ: 101046574
RNO: 367454
NGI: 103737200
HGL: 101708404
CFA: 449024(FUT5)
AML: 100468460
FCA: 101093666
PTG: 102972414(FUT3)
AJU: 106975444
BTA: 338077(FUT6)
BOM: 102271549(FUT6)
PHD: 102335338(FUT3)
CHX: 102181295(FUT3)
OAS: 101108745(FUT5)
SSC: 100513844
CFR: 102504974(FUT3)
BACU: 103005588(FUT3)
LVE: 103076464(FUT3)
OOR: 101276278
ECB: 111774191
MYB: 102255875(FUT3)
LAV: 100675894
MDO: 100015438(FUT3)
SHR: 100927060
GGA: 428341(FUT6)
MGP: 100544367
CJO: 107325644
APLA: 101802139
ACYG: 106048410
FPG: 101918531(FUT3)
FCH: 102058652(FUT3)
CLV: 102095283
EGZ: 104124394
AAM: 106492204
AMJ: 102561627(FUT3)
CMY: 102942494(FUT3)
CPIC: 101941134(FUT3)
ACS: 100565215
PVT: 110072709
PBI: 103063308(FUT3)
GJA: 107123994
XLA: 399049(fut6.S)
XTR: 100495376(fut6)
LCM: 102363620
ATH: AT1G71990(FUT13)
CRB: 17895957
BRP: 103831713
BOE: 106298421
THJ: 104809738
CPAP: 110813288
CIT: 102620994
TCC: 18592964
GRA: 105783399
DZI: 111276738
EGR: 104449507
VRA: 106770892
VAR: 108324168
CCAJ: 109812205
CAM: 101495537
ADU: 107467776
AIP: 107619064
LJA: Lj0g3v0164889.1(Lj0g3v0164889.1)
LANG: 109335938
FVE: 101297402
PPER: 18793901
PMUM: 103319172
PAVI: 110767076
ZJU: 107421412
CSV: 101216230
CMO: 103496555
MCHA: 111006550
RCU: 8262699
JCU: 105646728
HBR: 110649282
VVI: 100256631
SLY: 543777(FUT13)
SPEN: 107021151
SOT: 102590542
CANN: 107872770
NSY: 104229361
NTO: 104097007
INI: 109193282(a4Lea)
HAN: 110889492
LSV: 111918717
BVG: 104894700
SOE: 110787629
NNU: 104596822
DOSA: Os04t0432300-00(Os04g0432300) Os12t0170600-01(Os12g0170600)
BDI: 100828844
ATS: 109752008(LOC109752008)
SBI: 8076283
ZMA: 732742(a4Lea)
SITA: 101757637
PDA: 103701280
EGU: 105051880
DCT: 110098286
AOF: 109824399
ATR: 18436672
SMO: SELMODRAFT_412633(FucTB)
PPP: 112295359
HPY: HP0651
HPJ: jhp_0596(fucT)
HPG: HPG27_613
HPP: HPP12_0664(fucT)
HPB: HELPY_0720(futB) HELPY_1046(futA)
HPL: HPB8_427(fucT1) HPB8_428(fucT3) HPB8_853(fucT5) HPB8_854(fucT7)
HPI: hp908_0664(fucT) hp908_1067(fucU)
HPQ: hp2017_0641(fucT)
HPW: hp2018_0642(fucT)
HEF: HPF16_0660(fucT) HPF16_1018(fucT)
HPF: HPF30_0312(fucT) HPF30_0677(fucT)
HEQ: HPF32_0329(fucT) HPF32_0668(fucT)
HEX: HPF57_0674(fucT) HPF57_1038(fucT)
HPD: KHP_0670(fucTa) KHP_0977(fucTb)
HPYI: K750_05570
HAC: Hac_1063(fuc)
HCP: HCN_0898
MAGX: XM1_1803
MAGN: WV31_16795
NDO: DDD_2268
 » show all
Taxonomy
Reference
1  [PMID:7287719]
  Authors
Prieels JP, Monnom D, Dolmans M, Beyer TA, Hill RL.
  Title
Co-purification of the Lewis blood group N-acetylglucosaminide alpha 1 goes to 4 fucosyltransferase and an N-acetylglucosaminide alpha 1 goes to 3 fucosyltransferase from human milk.
  Journal
J Biol Chem 256:10456-63 (1981)
Reference
2  [PMID:10671538]
  Authors
Rasko DA, Wang G, Palcic MM, Taylor DE.
  Title
Cloning and characterization of the alpha(1,3/4) fucosyltransferase of Helicobacter pylori.
  Journal
J Biol Chem 275:4988-94 (2000)
DOI:10.1074/jbc.275.7.4988
  Sequence
[ag:AAF35291]
Reference
3  [PMID:12084979]
  Authors
Wilson IB.
  Title
Identification of a cDNA encoding a plant Lewis-type alpha1,4-fucosyltransferase.
  Journal
Glycoconj J 18:439-47 (2001)
  Sequence
[sly:543777]
Reference
4  [PMID:12676935]
  Authors
Ma B, Wang G, Palcic MM, Hazes B, Taylor DE.
  Title
C-terminal amino acids of Helicobacter pylori alpha1,3/4 fucosyltransferases determine type I and type II transfer.
  Journal
J Biol Chem 278:21893-900 (2003)
DOI:10.1074/jbc.M301704200
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.65
IUBMB Enzyme Nomenclature: 2.4.1.65
ExPASy - ENZYME nomenclature database: 2.4.1.65
BRENDA, the Enzyme Database: 2.4.1.65
CAS: 37277-69-3

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