KEGG   ENZYME: 2.5.1.148Help
Entry
EC 2.5.1.148                Enzyme                                 

Name
lycopaoctaene synthase;
LOS (gene name)
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
BRITE hierarchy
Sysname
geranylgeranyl diphosphate:geranylgeranyl diphosphate geranylgeranyltransferase
Reaction(IUBMB)
2 geranylgeranyl diphosphate + NADPH + H+ = lycopaoctaene + 2 diphosphate + NADP+ (overall reaction) [RN:R12281];
(1a) 2 geranylgeranyl diphosphate = diphosphate + prephytoene diphosphate [RN:R02065];
(1b) prephytoene diphosphate + NADPH + H+ = lycopaoctaene + diphosphate + NADP+ [RN:R12280]
Reaction(KEGG)
Substrate
geranylgeranyl diphosphate [CPD:C00353];
NADPH [CPD:C00005];
H+ [CPD:C00080];
prephytoene diphosphate [CPD:C03427]
Product
lycopaoctaene [CPD:C22055];
diphosphate [CPD:C00013];
NADP+ [CPD:C00006];
prephytoene diphosphate [CPD:C03427]
Comment
The enzyme, characterized from the green microalga Botryococcus braunii race L, in involved in biosynthesis of (14E,18E)-lycopadiene. In vitro, the enzyme can accept (2E,6E)-farnesyl diphosphate and phytyl diphosphate as substrates, and is also able to catalyse the condensation of two different substrate molecules, forming chimeric products. However, the use of these alternative substrates is not significant in vivo.
History
EC 2.5.1.148 created 2018
Orthology
K23152  lycopaoctaene synthase
Reference
1  [PMID:27050299]
  Authors
Thapa HR, Naik MT, Okada S, Takada K, Molnar I, Xu Y, Devarenne TP
  Title
A squalene synthase-like enzyme initiates production of tetraterpenoid hydrocarbons in Botryococcus braunii Race L.
  Journal
Nat Commun 7:11198 (2016)
DOI:10.1038/ncomms11198
  Sequence
Reference
2  [PMID:28813599]
  Authors
Thapa HR, Tang S, Sacchettini JC, Devarenne TP
  Title
Tetraterpene Synthase Substrate and Product Specificity in the Green Microalga Botryococcus braunii Race L.
  Journal
ACS Chem Biol 12:2408-2416 (2017)
DOI:10.1021/acschembio.7b00457
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.148
IUBMB Enzyme Nomenclature: 2.5.1.148
ExPASy - ENZYME nomenclature database: 2.5.1.148
BRENDA, the Enzyme Database: 2.5.1.148

DBGET integrated database retrieval system