KEGG   ENZYME: 2.6.1.100Help
Entry
EC 2.6.1.100                Enzyme                                 

Name
L-glutamine:2-deoxy-scyllo-inosose aminotransferase;
btrR (gene name);
neoB (gene name);
kanB (gene name)
Class
Transferases;
Transferring nitrogenous groups;
Transaminases
BRITE hierarchy
Sysname
L-glutamine:2-deoxy-scyllo-inosose aminotransferase
Reaction(IUBMB)
L-glutamine + 2-deoxy-scyllo-inosose = 2-oxoglutaramate + 2-deoxy-scyllo-inosamine [RN:R08890]
Reaction(KEGG)
Substrate
L-glutamine [CPD:C00064];
2-deoxy-scyllo-inosose [CPD:C17209]
Product
2-oxoglutaramate [CPD:C00940];
2-deoxy-scyllo-inosamine [CPD:C17580]
Comment
Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including kanamycin, butirosin, neomycin and ribostamycin. Also catalyses EC 2.6.1.101, L-glutamine:5-amino-2,3,4-trihydroxycyclohexanone aminotransferase [2].
History
EC 2.6.1.100 created 2013
Pathway
ec00524  Neomycin, kanamycin and gentamicin biosynthesis
ec01130  Biosynthesis of antibiotics
Orthology
K13547  L-glutamine:2-deoxy-scyllo-inosose/3-amino-2,3-dideoxy-scyllo-inosose aminotransferase
Genes
SRN: A4G23_05129(neoB)
Taxonomy
Reference
1  [PMID:12546424]
  Authors
Tamegai H, Eguchi T, Kakinuma K
  Title
First identification of Streptomyces genes involved in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics--genetic and evolutionary analysis of L-glutamine:2-deoxy-scyllo-inosose aminotransferase genes.
  Journal
J Antibiot (Tokyo) 55:1016-8 (2002)
Reference
2  [PMID:15827636]
  Authors
Huang F, Haydock SF, Mironenko T, Spiteller D, Li Y, Spencer JB
  Title
The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233: characterisation of an aminotransferase involved in the formation of 2-deoxystreptamine.
  Journal
Org Biomol Chem 3:1410-8 (2005)
DOI:10.1039/b501199j
Reference
3  [PMID:16506694]
  Authors
Kudo F, Yamamoto Y, Yokoyama K, Eguchi T, Kakinuma K.
  Title
Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773.
  Journal
J Antibiot (Tokyo) 58:766-74 (2005)
DOI:10.1038/ja.2005.104
  Sequence
Reference
4  [PMID:19219581]
  Authors
Jnawali HN, Subba B, Liou K, Sohng JK
  Title
Functional characterization of kanB by complementing in engineered Streptomyces fradiae Deltaneo6::tsr.
  Journal
Biotechnol Lett 31:869-75 (2009)
DOI:10.1007/s10529-009-9937-0
Other DBs
ExplorEnz - The Enzyme Database: 2.6.1.100
IUBMB Enzyme Nomenclature: 2.6.1.100
ExPASy - ENZYME nomenclature database: 2.6.1.100
BRENDA, the Enzyme Database: 2.6.1.100

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