Transferases;
Transferring nitrogenous groups;
Transaminases
BRITE hierarchy

L-glutamine:2-deoxy-scyllo-inosose aminotransferase

L-glutamine + 2-deoxy-scyllo-inosose = 2-oxoglutaramate + 2-deoxy-scyllo-inosamine [RN:R08890]

R08890
Reaction

L-glutamine [CPD:C00064];
2-deoxy-scyllo-inosose [CPD:C17209]

Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including kanamycin, butirosin, neomycin and ribostamycin. Also catalyses EC 2.6.1.101, L-glutamine:5-amino-2,3,4-trihydroxycyclohexanone aminotransferase [2].

EC 2.6.1.100 created 2013

1  [PMID:12546424]

Tamegai H, Eguchi T, Kakinuma K

First identification of Streptomyces genes involved in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics--genetic and evolutionary analysis of L-glutamine:2-deoxy-scyllo-inosose aminotransferase genes.

J Antibiot (Tokyo) 55:1016-8 (2002)
DOI:10.7164/antibiotics.55.1016

2  [PMID:15827636]

Huang F, Haydock SF, Mironenko T, Spiteller D, Li Y, Spencer JB

The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233: characterisation of an aminotransferase involved in the formation of 2-deoxystreptamine.

Org Biomol Chem 3:1410-8 (2005)
DOI:10.1039/b501199j

3  [PMID:16506694]

Kudo F, Yamamoto Y, Yokoyama K, Eguchi T, Kakinuma K

Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773.

J Antibiot (Tokyo) 58:766-74 (2005)
DOI:10.1038/ja.2005.104

[ag:BAD95819]

4  [PMID:19219581]

Jnawali HN, Subba B, Liou K, Sohng JK

Functional characterization of kanB by complementing in engineered Streptomyces fradiae Deltaneo6::tsr.

Biotechnol Lett 31:869-75 (2009)
DOI:10.1007/s10529-009-9937-0