KEGG ENZYME: 2.6.1.100

ENZYME: 2.6.1.100

Entry

EC 2.6.1.100                Enzyme

Name

L-glutamine:2-deoxy-scyllo-inosose aminotransferase;
btrR (gene name);
neoB (gene name);
kanB (gene name)

Class

Transferases;
Transferring nitrogenous groups;
Transaminases

Sysname

L-glutamine:2-deoxy-scyllo-inosose aminotransferase

Reaction(IUBMB)

L-glutamine + 2-deoxy-scyllo-inosose = 2-oxoglutaramate + 2-deoxy-scyllo-inosamine [RN:R08890]

Reaction(KEGG)

R08890

Substrate

L-glutamine [CPD:C00064];
2-deoxy-scyllo-inosose [CPD:C17209]

Product

2-oxoglutaramate [CPD:C00940];
2-deoxy-scyllo-inosamine [CPD:C17580]

Comment

Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including kanamycin, butirosin, neomycin and ribostamycin. Also catalyses EC 2.6.1.101, L-glutamine:5-amino-2,3,4-trihydroxycyclohexanone aminotransferase [2].

History

EC 2.6.1.100 created 2013

Pathway

ec00524

Neomycin, kanamycin and gentamicin biosynthesis

ec01110

Biosynthesis of secondary metabolites

Orthology

K13547

L-glutamine:2-deoxy-scyllo-inosose/3-amino-2,3-dideoxy-scyllo-inosose aminotransferase

Genes

PMUY:

KSS95_17140

PCEL:

HUB94_17990

MOT:	LTS72_25990

NTC:	KHQ06_25020

NHU:	H0264_36130

NYA:	LTV02_00430

SRN:	A4G23_05129(neoB)

SFB:	CP974_27370

AGRA:

AGRA3207_003346

UAM:	UABAM_05678

Reference

1 [PMID:12546424]

Authors

Tamegai H, Eguchi T, Kakinuma K

Title

First identification of Streptomyces genes involved in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics--genetic and evolutionary analysis of L-glutamine:2-deoxy-scyllo-inosose aminotransferase genes.

Journal

J Antibiot (Tokyo) 55:1016-8 (2002)
DOI:10.7164/antibiotics.55.1016

Reference

2 [PMID:15827636]

Authors

Huang F, Haydock SF, Mironenko T, Spiteller D, Li Y, Spencer JB

Title

The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233: characterisation of an aminotransferase involved in the formation of 2-deoxystreptamine.

Journal

Org Biomol Chem 3:1410-8 (2005)
DOI:10.1039/b501199j

Reference

3 [PMID:16506694]

Authors

Kudo F, Yamamoto Y, Yokoyama K, Eguchi T, Kakinuma K

Title

Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773.

Journal

J Antibiot (Tokyo) 58:766-74 (2005)
DOI:10.1038/ja.2005.104

Sequence

[ag:BAD95819]