| Entry |
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| Name |
L-glutamine:2-deoxy-scyllo-inosose aminotransferase;
btrR (gene name);
neoB (gene name);
kanB (gene name)
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| Class |
Transferases;
Transferring nitrogenous groups;
Transaminases
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| Sysname |
L-glutamine:2-deoxy-scyllo-inosose aminotransferase
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| Reaction(IUBMB) |
L-glutamine + 2-deoxy-scyllo-inosose = 2-oxoglutaramate + 2-deoxy-scyllo-inosamine [RN: R08890]
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| Reaction(KEGG) |
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| Substrate |
L-glutamine [CPD: C00064];
2-deoxy-scyllo-inosose [CPD: C17209]
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| Product |
2-oxoglutaramate [CPD: C00940];
2-deoxy-scyllo-inosamine [CPD: C17580]
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| Comment |
Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including kanamycin, butirosin, neomycin and ribostamycin. Also catalyses EC 2.6.1.101, L-glutamine:5-amino-2,3,4-trihydroxycyclohexanone aminotransferase [2].
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| History |
EC 2.6.1.100 created 2013
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| Pathway |
| ec00524 | Neomycin, kanamycin and gentamicin biosynthesis |
| ec01110 | Biosynthesis of secondary metabolites |
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| Orthology |
| K13547 | L-glutamine:2-deoxy-scyllo-inosose/3-amino-2,3-dideoxy-scyllo-inosose aminotransferase |
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| Genes |
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| Reference |
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| Authors |
Tamegai H, Eguchi T, Kakinuma K |
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| Title |
First identification of Streptomyces genes involved in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics--genetic and evolutionary analysis of L-glutamine:2-deoxy-scyllo-inosose aminotransferase genes. |
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| Journal |
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| Reference |
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| Authors |
Huang F, Haydock SF, Mironenko T, Spiteller D, Li Y, Spencer JB |
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| Title |
The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233: characterisation of an aminotransferase involved in the formation of 2-deoxystreptamine. |
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| Journal |
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| Reference |
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| Authors |
Kudo F, Yamamoto Y, Yokoyama K, Eguchi T, Kakinuma K. |
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| Title |
Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Jnawali HN, Subba B, Liou K, Sohng JK |
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| Title |
Functional characterization of kanB by complementing in engineered Streptomyces fradiae Deltaneo6::tsr. |
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| Journal |
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| Other DBs |
| ExPASy - ENZYME nomenclature database: | 2.6.1.100 |
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