Acting on ester bonds;
Endodeoxyribonucleases producing 5'-phosphomonoesters
Endonucleolytic nicking and cleavage of cytosine-containing double-stranded DNA.
Requires Mg2+. This phage T4 enzyme is involved in degradation of host DNA. The enzyme primarily catalyses nicking of the bottom strand of double stranded DNA between the first and second base pair to the right of a top-strand CCGC motif. Double-stranded breaks are produced 5- to 10-fold less frequently . It does not cleave the T4 native DNA, which contains 5-hydroxymethylcytosine instead of cytosine.