KEGG   ENZYME: 3.1.3.76
Entry
EC 3.1.3.76                 Enzyme                                 
Name
lipid-phosphate phosphatase;
hydroxy fatty acid phosphatase;
dihydroxy fatty acid phosphatase;
hydroxy lipid phosphatase;
sEH (ambiguous);
soluble epoxide hydrolase (ambiguous);
(9S,10S)-10-hydroxy-9-(phosphonooxy)octadecanoate phosphohydrolase
Class
Hydrolases;
Acting on ester bonds;
Phosphoric-monoester hydrolases
Sysname
(9S,10S)-10-hydroxy-9-(phosphooxy)octadecanoate phosphohydrolase
Reaction(IUBMB)
(9S,10S)-10-hydroxy-9-(phosphooxy)octadecanoate + H2O = (9S,10S)-9,10-dihydroxyoctadecanoate + phosphate [RN:R07582]
Reaction(KEGG)
R07582
Substrate
(9S,10S)-10-hydroxy-9-(phosphooxy)octadecanoate;
H2O [CPD:C00001]
Product
(9S,10S)-9,10-dihydroxyoctadecanoate [CPD:C15988];
phosphate [CPD:C00009]
Comment
Requires Mg2+ for maximal activity. The enzyme from mammals is a bifunctional enzyme: the N-terminal domain exhibits lipid-phosphate-phosphatase activity and the C-terminal domain has the activity of EC 3.3.2.10, soluble epoxide hydrolase (sEH) [1]. The best substrates for this enzyme are 10-hydroxy-9-(phosphooxy)octadecanoates, with the threo- form being a better substrate than the erythro- form [1]. The phosphatase activity is not found in plant sEH or in EC 3.3.2.9, microsomal epoxide hydrolase, from mammals [1].
History
EC 3.1.3.76 created 2006
Orthology
K08726  soluble epoxide hydrolase / lipid-phosphate phosphatase
Genes
HSA2053(EPHX2)
PTR464074(EPHX2)
PPS100990932(EPHX2)
GGO101136750(EPHX2)
PON100172736(EPHX2)
NLE100585861(EPHX2)
MCC712926(EPHX2)
MCF102134633(EPHX2)
CSAB103215526(EPHX2)
CATY105579174(EPHX2)
PANU101025043(EPHX2)
TGE112629928(EPHX2)
RRO104673945(EPHX2)
RBB108537568(EPHX2)
TFN117093881(EPHX2)
PTEH111545735(EPHX2)
CJC100402999(EPHX2)
SBQ101033420(EPHX2)
CSYR103250514(EPHX2)
MMUR105877163(EPHX2)
OGA100958729(EPHX2)
MMU13850(Ephx2)
MCAL110309184(Ephx2)
MPAH110325104(Ephx2)
RNO65030(Ephx2)
MCOC116084507(Ephx2)
MUN110559220(Ephx2)
CGE100757328(Ephx2)
PLEU114707310(Ephx2)
NGI103730002(Ephx2)
HGL101715352(Ephx2)
CPOC100734490(Ephx2)
CCAN109683973
DORD105991601(Ephx2)
DSP122123504(Ephx2)
OCU100350230(EPHX2)
OPI101526632(EPHX2)
TUP102480725(EPHX2)
CFA477373(EPHX2)
VVP112926786(EPHX2)
VLG121496796(EPHX2)
AML100483572(EPHX2)
UMR103675990(EPHX2)
UAH113269956(EPHX2)
UAR123777357(EPHX2)
ELK111152645
LLV125092972
MPUF101689697(EPHX2)
ORO101373863(EPHX2)
EJU114217531(EPHX2)
ZCA113924682(EPHX2)
MLX118022112(EPHX2)
FCA101092355(EPHX2)
PYU121022524(EPHX2)
PBG122473878(EPHX2)
PTG102957184(EPHX2)
PPAD109262341(EPHX2)
AJU106973110(EPHX2)
HHV120220308(EPHX2)
BTA511716(EPHX2)
BOM102267808(EPHX2)
BIU109563159(EPHX2)
BBUB102411270(EPHX2)
CHX102185835(EPHX2)
OAS101106654(EPHX2)
ODA120860355 120866098
CCAD122431866(EPHX2)
SSC414425(EPHX2)
CFR102517269(EPHX2)
CBAI105063866(EPHX2)
CDK105102229(EPHX2)
VPC102535491(EPHX2)
BACU103015729(EPHX2)
LVE103082628(EPHX2)
OOR101282222(EPHX2)
DLE111185405(EPHX2)
PCAD102997018(EPHX2)
ECB100060414(EPHX2)
EPZ103550424(EPHX2)
EAI106832514(EPHX2)
MYB102255588(EPHX2)
MYD102755564(EPHX2)
MMYO118659062(EPHX2)
MLF102426648(EPHX2)
MNA107538569(EPHX2)
PKL118710951(EPHX2)
HAI109379561(EPHX2)
DRO112312409(EPHX2)
SHON118984357(EPHX2)
AJM119044629
PDIC114503007(EPHX2)
PHAS123813726(EPHX2)
RFQ117037789(EPHX2)
PALE102886924(EPHX2)
PGIG120613418(EPHX2)
PVP105292223(EPHX2)
RAY107507539(EPHX2)
MJV108394328(EPHX2)
TOD119258012(EPHX2)
SARA101555407(EPHX2)
LAV100658579(EPHX2)
TMU101343713
DNM101446323(EPHX2)
MDO100030580(EPHX2)
GAS123233526(EPHX2)
SHR100931196 100931457 100931720
PCW110206532(EPHX2)
OAA100093187(EPHX2)
GGA421999(EPHX2)
PCOC116235639(EPHX2)
MGP100550075(EPHX2)
CJO107312419(EPHX2)
NMEL110396327(EPHX2)
APLA101802271(EPHX2)
AFUL116487971(EPHX2)
TGU115494601(EPHX2)
LSR110477399(EPHX2)
SCAN103825122(EPHX2)
OTC121344673(EPHX2)
FAB101820851(EPHX2)
PHI102113903(EPHX2)
CCAE111927478(EPHX2)
FPG101922601(EPHX2)
FCH102047869(EPHX2)
CLV102084991(EPHX2)
EGZ104132029(EPHX2)
NNI104014768(EPHX2)
ACUN113478826(EPHX2)
TALA116959311(EPHX2)
ACHC115351280(EPHX2)
AAM106486373(EPHX2)
AROW112966641(EPHX2)
NPD112949124(EPHX2)
DNE112993182(EPHX2)
ASN102382847(EPHX2)
AMJ102558789(EPHX2)
CPOO109311562(EPHX2)
PSS102463793(EPHX2)
CMY102944771(EPHX2)
CPIC101948727(EPHX2)
CABI116830887 116837371
MRV120401417 120402424
ACS100564154(ephx2)
PVT110089501(EPHX2)
SUND121921147(EPHX2)
PBI103065207
PMUR107296270
TSR106551030(EPHX2)
PGUT117667686(EPHX2)
VKO123033639(EPHX2)
PMUA114594946(EPHX2)
ZVI118082948(EPHX2)
GJA107118053(EPHX2)
XLA447032(ephx2.L)
XTR448759(ephx2)
NPR108798538(EPHX2)
RTEM120936048(EPHX2)
BBUF120997151(EPHX2)
BGAR122934206(EPHX2)
DRE494099(ephx2)
SRX107717245(ephx2) 107733988
SANH107653762 107692411
SGH107552031 107594076(ephx2)
CCAR109107424
CAUA113060837 113117731
IPU108270338(ephx2)
PHYP113538653(ephx2)
SMEO124390354(ephx2)
AMEX103037948(ephx2)
EEE113572683(ephx2)
TRU101078765(ephx2)
TNGGSTEN00019421G001
LCO104928568(ephx2)
NCC104958265(ephx2)
ELY117250024(ephx2)
PLEP121939287(ephx2)
SLUC116041392
ECRA117939678(ephx2)
PFLV114571310(ephx2)
GAT120818346(ephx2)
PPUG119220354(ephx2)
MSAM119892822 119901244 119901687 119901935 119903463 119915610
CUD121506165(ephx2)
MZE101483599(ephx2)
ONL100705182(ephx2)
OAU116325329(ephx2)
OLA101157276(ephx2)
OML112156764(ephx2)
XMA102235090(ephx2)
XCO114140564(ephx2)
XHE116715605(ephx2)
PRET103456621(ephx2)
PFOR103154111(ephx2)
PLAI106949506(ephx2)
PMEI106919577(ephx2)
GAF122826945(ephx2)
CVG107096825(ephx2)
CTUL119794676(ephx2)
GMU124861934(ephx2)
NFU107384625(ephx2)
KMR108246829(ephx2)
ALIM106527033(ephx2)
NWH119428662(ephx2)
AOCE111573235(ephx2)
LCF108873166(ephx2)
SDU111234755(ephx2)
SLAL111672419(ephx2)
XGL120798362(ephx2)
MALB109955803(ephx2)
SASA106604359(HYES) 106611412(HYES)
OTW112239012 112239182 112256950
OMY110498218(ephx2) 110504256
OGO124001944(ephx2) 124046757
ONE115113794 115120915
SALP112071651(ephx2)
SNH120023101(ephx2)
ELS105028738(ephx2)
AANG118209863(ephx2)
LOC102697721(ephx2)
PSPA121317357(ephx2)
ARUT117411030(ephx2)
BFO118420112
BBEL109472833
CIN113474027
SCLV120337441 120337631
SPU590472(EH1) 752510(eh2)
APLC110985936 110985937
SKO100367532
NVE5521988
EPA110251610
ATEN116301187
PDAM113686980
SPIS111334437
TADTRIADDRAFT_30313 TRIADDRAFT_59995 TRIADDRAFT_60010 TRIADDRAFT_60079 TRIADDRAFT_64238
DZI111302718
ADU107492180
PXB103939771
CMAX111482470
QSU111990796 111992415 112014879 112037600
HAN110941524
CCAV112528667
BDI100841009
TDC119283936
SBI8071538
YLIYALI0A01441g YALI0C23224g YALI0E19899g
NCRNCU02924
NTENEUTE1DRAFT119163(NEUTE1DRAFT_119163)
SMPSMAC_06471 SMAC_12126
PANPODANSg2586 PODANSg4881
TTTTHITE_2119426
MTMMYCTH_2304288
CTHRCTHT_0024670
MGRMGG_05175 MGG_05826 MGG_07954 MGG_09603 MGG_13875
TMNUCRPA7_1749
SSCKSPSK_04392
FGRFGSG_06744 FGSG_11196
FPUFPSE_02822 FPSE_09090
FVRFVEG_12217
FOXFOXG_08935 FOXG_10109 FOXG_13323 FOXG_14841 FOXG_19442 FOXG_22390
NHENECHADRAFT_30431 NECHADRAFT_38604 NECHADRAFT_51532 NECHADRAFT_98121
TRETRIREDRAFT_53220 TRIREDRAFT_57045
TRRM419DRAFT_69084 M419DRAFT_72359
MAWMAC_03724
MAJMAA_05891 MAA_08508
CMTCCM_01660 CCM_08720
PLJVFPFJ_10414
VALVDBG_05347 VDBG_08907
VDAVDAG_06569 VDAG_06997
CFJCFIO01_01948 CFIO01_02421 CFIO01_04356 CFIO01_06499 CFIO01_11995
ELAUCREL1_10468 UCREL1_2213 UCREL1_3351 UCREL1_9854
PFYPFICI_03331 PFICI_05569 PFICI_08381 PFICI_14790
SSLSS1G_08093 SS1G_13652
BFUBCIN_10g01840 BCIN_13g00770 BCIN_15g00230
MBEMBM_01675 MBM_08747
PSCOLY89DRAFT_507394 LY89DRAFT_594279 LY89DRAFT_662388 LY89DRAFT_683181 LY89DRAFT_702826 LY89DRAFT_785580
GLZGLAREA_02712 GLAREA_04148 GLAREA_09895 GLAREA_12612
AORAO090012000395
ANGANI_1_1944014(An01g14420)
TMFPMAA_099480
TRGTRUGW13939_04437 TRUGW13939_05195 TRUGW13939_08349
PBLPAAG_06941
PBNPADG_01474
AJEHCAG_03262 HCAG_06274
PNOSNOG_12792 SNOG_13782(SNOG_13781) SNOG_14994
PTEPTT_11179 PTT_13711
BZECOCCADRAFT_10479 COCCADRAFT_23112 COCCADRAFT_24314
BSCCOCSADRAFT_329080 COCSADRAFT_37209
BORCOCMIDRAFT_26566 COCMIDRAFT_3129 COCMIDRAFT_97004
AALTCC77DRAFT_1020259 CC77DRAFT_1067778 CC77DRAFT_23691 CC77DRAFT_924398 CC77DRAFT_933869 CC77DRAFT_991934
ZTRMYCGRDRAFT_101398 MYCGRDRAFT_45185 MYCGRDRAFT_67922 MYCGRDRAFT_69862 MYCGRDRAFT_74004
PFJMYCFIDRAFT_186960 MYCFIDRAFT_202874 MYCFIDRAFT_31012 MYCFIDRAFT_35544 MYCFIDRAFT_63852
BCOMBAUCODRAFT_96023
NPAUCRNP2_1253 UCRNP2_2402 UCRNP2_2781 UCRNP2_536 UCRNP2_578 UCRNP2_7692 UCRNP2_9159
TMLGSTUM_00010225001
PPLPOSPLDRAFT_103326
TVSTRAVEDRAFT_114934 TRAVEDRAFT_116461 TRAVEDRAFT_121619 TRAVEDRAFT_163026 TRAVEDRAFT_171945 TRAVEDRAFT_42938 TRAVEDRAFT_42939 TRAVEDRAFT_42940 TRAVEDRAFT_42941 TRAVEDRAFT_42942 TRAVEDRAFT_60092 TRAVEDRAFT_72850 TRAVEDRAFT_94256
DSQDICSQDRAFT_101446 DICSQDRAFT_104823 DICSQDRAFT_61223
PCOPHACADRAFT_104575 PHACADRAFT_118086 PHACADRAFT_151144 PHACADRAFT_191247 PHACADRAFT_194943 PHACADRAFT_198096 PHACADRAFT_207661 PHACADRAFT_208465 PHACADRAFT_255121 PHACADRAFT_260148 PHACADRAFT_91351 PHACADRAFT_93100
SHSSTEHIDRAFT_106361 STEHIDRAFT_122857 STEHIDRAFT_61089 STEHIDRAFT_80676 STEHIDRAFT_81610
HIRHETIRDRAFT_148090 HETIRDRAFT_155125 HETIRDRAFT_156748 HETIRDRAFT_156749 HETIRDRAFT_174637 HETIRDRAFT_63479
PSQPUNSTDRAFT_112781 PUNSTDRAFT_116520 PUNSTDRAFT_139547 PUNSTDRAFT_98990
ADLAURDEDRAFT_110586 AURDEDRAFT_117379 AURDEDRAFT_117569 AURDEDRAFT_117572 AURDEDRAFT_152313 AURDEDRAFT_175477 AURDEDRAFT_80706
FMEFOMMEDRAFT_106649 FOMMEDRAFT_127219 FOMMEDRAFT_135095
GTRGLOTRDRAFT_45684 GLOTRDRAFT_68350
LBCLACBIDRAFT_303899 LACBIDRAFT_311540(LbEPHX1)
MPRMPER_04928 MPER_09275 MPER_11089
MRRMoror_11208 Moror_13472 Moror_13512 Moror_13860 Moror_14385 Moror_14388 Moror_16097 Moror_2330 Moror_2947 Moror_4070 Moror_6712 Moror_8127
CCICC1G_00644 CC1G_00645 CC1G_14690
SCMSCHCODRAFT_12753 SCHCODRAFT_235079 SCHCODRAFT_59770 SCHCODRAFT_62514
ABPAGABI1DRAFT110229(AGABI1DRAFT_110229) AGABI1DRAFT38499(AGABI1DRAFT_38499) AGABI1DRAFT70817(AGABI1DRAFT_70817)
ABVAGABI2DRAFT184905(AGABI2DRAFT_184905) AGABI2DRAFT189875(AGABI2DRAFT_189875) AGABI2DRAFT78715(AGABI2DRAFT_78715)
CPUTCONPUDRAFT_122227 CONPUDRAFT_93019
SLASERLADRAFT_354546 SERLADRAFT_448219 SERLADRAFT_479064 SERLADRAFT_479070 SERLADRAFT_480679
WSEWALSEDRAFT_65881
WICJ056_000814
UMAUMAG_04063
PFPPFL1_00989
PGRPGTG_00491 PGTG_05962 PGTG_05973
MLRMELLADRAFT_102079 MELLADRAFT_115158 MELLADRAFT_123933
ACANACA1_322160
PIFPITG_11920 PITG_11921 PITG_11922 PITG_15955 PITG_15956
PSOJPHYSODRAFT_314147 PHYSODRAFT_318677 PHYSODRAFT_525117 PHYSODRAFT_558062 PHYSODRAFT_564652
SPARSPRG_06039 SPRG_06040 SPRG_06041
HAAA5892_09075
MASYDPH57_08340
SCHSphch_3450
KBSEPA93_04525
TBHTbon_07320
 » show all
Reference
1  [PMID:12574510]
  Authors
Newman JW, Morisseau C, Harris TR, Hammock BD
  Title
The soluble epoxide hydrolase encoded by EPXH2 is a bifunctional enzyme with novel lipid phosphate phosphatase activity.
  Journal
Proc Natl Acad Sci U S A 100:1558-63 (2003)
DOI:10.1073/pnas.0437724100
  Sequence
[hsa:2053]
Reference
2  [PMID:12574508]
  Authors
Cronin A, Mowbray S, Durk H, Homburg S, Fleming I, Fisslthaler B, Oesch F, Arand M.
  Title
The N-terminal domain of mammalian soluble epoxide hydrolase is a phosphatase.
  Journal
Proc Natl Acad Sci U S A 100:1552-7 (2003)
DOI:10.1073/pnas.0437829100
Reference
3  [PMID:15822179]
  Authors
Morisseau C, Hammock BD.
  Title
Epoxide hydrolases: mechanisms, inhibitor designs, and biological roles.
  Journal
Annu Rev Pharmacol Toxicol 45:311-33 (2005)
DOI:10.1146/annurev.pharmtox.45.120403.095920
Reference
4  [PMID:16142916]
  Authors
Tran KL, Aronov PA, Tanaka H, Newman JW, Hammock BD, Morisseau C.
  Title
Lipid sulfates and sulfonates are allosteric competitive inhibitors of the N-terminal phosphatase activity of the mammalian soluble epoxide hydrolase.
  Journal
Biochemistry 44:12179-87 (2005)
DOI:10.1021/bi050842g
Reference
5  [PMID:15748653]
  Authors
Newman JW, Morisseau C, Hammock BD.
  Title
Epoxide hydrolases: their roles and interactions with lipid metabolism.
  Journal
Prog Lipid Res 44:1-51 (2005)
DOI:10.1016/j.plipres.2004.10.001
  Sequence
[hsa:2053]
Reference
6  [PMID:15196990]
  Authors
Srivastava PK, Sharma VK, Kalonia DS, Grant DF.
  Title
Polymorphisms in human soluble epoxide hydrolase: effects on enzyme activity, enzyme stability, and quaternary structure.
  Journal
Arch Biochem Biophys 427:164-9 (2004)
DOI:10.1016/j.abb.2004.05.003
Reference
7  [PMID:15096040]
  Authors
Gomez GA, Morisseau C, Hammock BD, Christianson DW.
  Title
Structure of human epoxide hydrolase reveals mechanistic inferences on bifunctional catalysis in epoxide and phosphate ester hydrolysis.
  Journal
Biochemistry 43:4716-23 (2004)
DOI:10.1021/bi036189j
  Sequence
[hsa:2053]
Other DBs
ExplorEnz - The Enzyme Database: 3.1.3.76
IUBMB Enzyme Nomenclature: 3.1.3.76
ExPASy - ENZYME nomenclature database: 3.1.3.76
BRENDA, the Enzyme Database: 3.1.3.76

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