a protopanaxadiol-type ginsenoside with two glucosyl residues at position 3 + 2 H2O = a protopanaxadiol-type ginsenoside with no glycosidic modification at position 3 + 2 D-glucopyranose (overall reaction);
(1a) a protopanaxadiol-type ginsenoside with two glucosyl residues at position 3 + H2O = a protopanaxadiol-type ginsenoside with one glucosyl residue at position 3 + D-glucopyranose;
(1b) a protopanaxadiol-type ginsenoside with one glucosyl residue at position 3 + H2O = a protopanaxadiol-type ginsenoside with no glycosidic modification at position 3 + D-glucopyranose
Substrate
protopanaxadiol-type ginsenoside with two glucosyl residues at position 3;
H2O [CPD:C00001];
protopanaxadiol-type ginsenoside with one glucosyl residue at position 3
Product
protopanaxadiol-type ginsenoside with no glycosidic modification at position 3;
D-glucopyranose [CPD:C00031];
protopanaxadiol-type ginsenoside with one glucosyl residue at position 3
Comment
Ginsenosidase type III catalyses the sequential hydrolysis of the 3-O-beta-D-(1->2)-glucopyranosyl bond followed by hydrolysis of the 3-O-beta-D-glucopyranosyl bond of protopanaxadiol ginsenosides. When acting for example on ginsenoside Rb1 the enzyme first generates ginsenoside XVII, and subsequently ginsenoside LXXV.
An DS, Cui CH, Lee HG, Wang L, Kim SC, Lee ST, Jin F, Yu H, Chin YW, Lee HK, Im WT, Kim SG
Title
Identification and characterization of a novel Terrabacter ginsenosidimutans sp. nov. beta-glucosidase that transforms ginsenoside Rb1 into the rare gypenosides XVII and LXXV.
Enzymatic Biotransformation of Ginsenoside Rb1 and Gypenoside XVII into Ginsenosides Rd and F2 by Recombinant beta-glucosidase from Flavobacterium johnsoniae.
