KEGG ENZYME: 3.5.2.18

ENZYME: 3.5.2.18

Entry

EC 3.5.2.18                 Enzyme

Name

enamidase

Class

Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In cyclic amides

Sysname

6-oxo-1,4,5,6-tetrahydronicotinate amidohydrolase

Reaction(IUBMB)

6-oxo-1,4,5,6-tetrahydronicotinate + 2 H2O = 2-formylglutarate + NH3 [RN:R07984]

Reaction(KEGG)

R07984

Substrate

6-oxo-1,4,5,6-tetrahydronicotinate [CPD:C04226];
H2O [CPD:C00001]

Product

2-formylglutarate [CPD:C16159];
NH3 [CPD:C00014]

Comment

Contains iron and Zn2+. Forms part of the nicotinate-fermentation catabolism pathway in Eubacterium barkeri. Other enzymes involved in this pathway are EC 1.17.1.5 (nicotinate dehydrogenase), EC 1.3.7.1 (6-hydroxynicotinate reductase), EC 1.1.1.291 (2-hydroxymethylglutarate dehydrogenase), EC 5.4.99.4 (2-methyleneglutarate mutase), EC 5.3.3.6 (methylitaconate Delta-isomerase), EC 4.2.1.85 (dimethylmaleate hydratase) and EC 4.1.3.32 (2,3-dimethylmalate lyase).

History

EC 3.5.2.18 created 2006

Pathway

ec00760

Nicotinate and nicotinamide metabolism

ec01120

Microbial metabolism in diverse environments

Orthology

K15358

enamidase

Genes

GAI:	IMCC3135_06255(ena)

BUG:	BC1001_4282

BGF:	BC1003_5118

BUQ:	AC233_28060

BXE:

Bxe_B0661

BXB:	DR64_5992(ena)

BPX:	BUPH_00866

BPY:	Bphyt_4330

BUZ:	AYM40_31820

BFN:

OI25_6348

PSPW:

BJG93_23415

PARB:

CJU94_26125

PGP:	CUJ91_28155

PCJ:	CUJ87_25415

PTS:	CUJ90_21655

PCAF:

DSC91_000567

PMEG:

FNZ07_07695

PDIO:

PDMSB3_2713.1(Ena)

PBRY:

NDK50_24140

PKF:	RW095_17625

POLY:

PSHI2_17100

POLN:

PSHI8_17110

BTRM:

SAMEA390648703552(Ena)

PUD:	G9Q38_02600

ODH:	DHf2319_11960

PIG:	EGT29_06335

NARM:

N7E01_07695

RHY:	RD110_24235

POL:

Bpro_4072

ACK:	C380_01125

ARAD:

KI609_01135

ACIV:

EXV95_17660

VEI:

Veis_0850

VAP:	Vapar_0175

VPE:	Varpa_0204

VPD:	VAPA_1c02110

VAA:	AX767_09800

VBO:	CKY39_01120

VAM:	C4F17_17950

VAZ:	H7F35_13040

VAV:	IG196_31895

HYN:	F9K07_03470

DRG:	H9K76_16630

HGR:	DW355_05230

ATER:

MW290_16305

MCI:	Mesci_2473

MCIC:

A4R28_29555

MOP:	Mesop_5964

MESW:

A9K65_012940

MERD:

EB233_26860

MMEI:

LRP31_30505

KMN:	HW532_02545

RGA:	RGR602_PC02240

KAI:

K32_06710

BJA:	bll3830(bll3830)

BJU:	BJ6T_60250

BJP:	RN69_29190

BRA:

BRADO2124

BBT:

BBta_2433

BRS:

S23_44100

AOL:

S58_54100

BRC:	BCCGELA001_15920

BRAD:

BF49_1213

BIC:	LMTR13_29485

BRO:	BRAD285_5137

BRK:	CWS35_16985

BOT:	CIT37_34745

BRQ:	CIT40_11960

BGQ:	X265_24160

BGZ:	XH91_21345

BSYM:

CIT39_11200

BBET:

F8237_03060

BARH:

WN72_20570

BVZ:	BRAD3257_5206(Ena)

BEL:	BE61_72760

BDG:	LPJ38_21325

BSEP:

HAP48_0029650

BQB:	J4P68_0030920

BSEI:

KMZ68_19995

BBAN:

J4G43_021150

BCAN:

BcanWSM471_16605

BAUT:

QA635_29685

BBRA:

QA636_31590

BGK:	IC762_10265

BCOU:

IC761_21920

BPAH:

QA639_14295

BXN:	I3J27_15215

BROS:

QUH67_26890

BRAZ:

LRP30_16575

BOY:	LQG66_13505

BRAY:

ML401_12450

RPB:

RPB_1671

RHOL:

RBJ75_19300

TRB:	HB776_00735

BVV:	BHK69_21650

BVES:

QO058_17435

BOP:	AXW83_07765

BOS:	BSY19_2959

BOI:	BLM15_19740

BOW:	NWE53_15920

BOSE:

RMR04_12195

XAU:

Xaut_4206

XDI:	EZH22_12930 EZH22_19775

AZC:

AZC_2804

LNE:	FZC33_27045

MOG:	MMB17_14920

MICO:

GDR74_16735

CHEL:

AL346_07315

CDQ:	BOQ54_00455

HMC:	HYPMC_0271

RHZ:	RHPLAN_22690

DEA:	FPZ08_13555

MHAR:

L1P08_16275

HDI:	HDIA_4511(Ena)

AUZ:	Sa4125_40670

JIE:	OH818_26770

PSIN:

CAK95_27655

LIZ:	LGH83_07260

SIL:

SPO0819

RCON:

K3740_05035

RDE:

RD1_1529

RLI:	RLO149_c031690

OAT:	OAN307_c22910

OTM:

OSB_05860

SPOT:

G6548_15185

SMED:

JNX03_20265

SULB:

IV89_003274

ROM:	EI983_16030

TGL:	HFZ77_04290

PARS:

DRW48_13380

SEDI:

EBB79_04765

PPSO:

QPJ95_02720

PAMO:

BAR1_16990

AMYL:

QBD29_06480

AMAQ:

GO499_18845

BOSG:

GKR98_17710

GXL:

H845_1049

KEU:	S101446_01668

SRF:	LHU95_08660 LHU95_21430

DEX:	HWD60_11945

DVN:	HQ394_03990

MAG:

amb1486

HTQ:	FRZ44_34620

HADH:

FRZ61_33820

ELIO:

KO353_13270

DTI:	Desti_0417

DMP:

FAK_19840

CPAT:

CLPA_c02940(ena)

CPAE:

CPAST_c02940(ena)

ACOL:

K5I23_08380 K5I23_11290

DOR:	Desor_0447

AACX:

DEACI_1470

ACAE:

HYG86_14535

PROM:

QO263_18015

TEP:	TepRe1_0407 TepRe1_0414

TAE:	TepiRe1_0451(Ena) TepiRe1_0461(Ena)

NTH:	Nther_2144

STED:

SPTER_39200(ena)

SSPH:

SPSPH_035220(ena_1) SPSPH_035230(ena_2)

SOVA:

SOV_13270(Ena)

SSID:

SPSIL_018510(ena)

NTP:	CRH09_08320

SDEC:

L3078_00380

LTR:	EVS81_03855

LTN:	KVY00_08460

HEH:	L3i23_22570

STRR:

EKD16_09270(ena)

ARHD:

VSH64_07450

SAQ:

Sare_0577

EKE:	EK0264_06290

CWO:

Cwoe_3945

TRO:

trd_A0335

IPO:	Ilyop_0117

CSU:	CSUB_C0238 CSUB_C0382

» show all

Reference

1 [PMID:16894175]

Authors

Alhapel A, Darley DJ, Wagener N, Eckel E, Elsner N, Pierik AJ.

Title

Molecular and functional analysis of nicotinate catabolism in Eubacterium barkeri.

Journal

Proc Natl Acad Sci U S A 103:12341-6 (2006)
DOI:10.1073/pnas.0601635103

Sequence

[ag:ABC88401]

Other DBs

ExplorEnz - The Enzyme Database:

3.5.2.18

IUBMB Enzyme Nomenclature:

3.5.2.18

ExPASy - ENZYME nomenclature database:

3.5.2.18

BRENDA, the Enzyme Database:

3.5.2.18

DBGET integrated database retrieval system