Entry
Name
mRNA 5'-phosphatase;
5'-polynucleotidase;
polynucleotide 5'-phosphohydrolase;
RNGTT (gene name);
CET1 (gene name);
mRNA 5'-triphosphate monophosphatase
Class
Hydrolases;
Acting on acid anhydrides;
In phosphorus-containing anhydrides
BRITE hierarchy
Sysname
5'-triphospho-mRNA 5'-phosphohydrolase
Reaction(IUBMB)
a 5'-triphospho-[mRNA] + H2O = a 5'-diphospho-[mRNA] + phosphate
Substrate
Product
Comment
The enzyme, found in eukaryotes and some plus strand RNA viruses (e.g. alphavirus), is involved in mRNA capping. Unlike the eukaryotic enzyme, the viral enzyme requires a purine in the first position of the mRNA. The human enzyme is a multi domain protein that also has the activity of EC
2.7.7.50 , mRNA guanylyltransferase.
History
EC 3.6.1.74 created 2021
Orthology
K01098 polynucleotide 5'-triphosphatase
Genes
CGE : 100770876(Rngtt) 100773893
PLEU : 114681721 114695435(Rngtt)
OPI : 101535862 101536652(RNGTT)
MDT : 132237076(RNGTT) 132240057
TVP : 118855719 118857144(RNGTT)
CCAE : 111925738 111925739
SRX : 107731501 107742988(rngtt)
SANH : 107660510 107680298(rngtt)
CAUA : 113037664 113063493
CGIB : 127938312 127984360
MASI : 127409895 127415814
DME : Dmel_CG1810(mRNA-cap)
DSI : Dsimw501_GD15855(Dsim_GD15855)
AMER : 121591751 121601242
BCOO : 119070255 119076789
AGIF : 122852653 122855234 122855508 122855563
CINS : 118064162 118064873 118065309 118065310 118065311 118065313 118065452 118065604 118066103 118066736 118066737 118067054 118069779 118069792 118069793 118069851 118069900 118071350 118073530
CFEL : 113373286 113374041
HHAL : 106691572(mRNA-cap)
DPX : DAPPUDRAFT_128585 DAPPUDRAFT_303937
DMK : 116919835 116934547 116936452 123468033 123470576
DPZ : 124312967 124338448 124343634
PPOI : 119095886 119095888
CBR : CBG_03700(Cbr-cel-1) CBG_03761
RPHI : 132724977 132748005
ATH : AT3G09100 AT5G01290 AT5G28210
ALY : 9307003 9308379 9320779
CRB : 17881235 17882849 17890698
CSAT : 104708977 104734174 104737788 104745076 104749285 104764607 104768335
EUS : EUTSA_v10000074mg EUTSA_v10005507mg EUTSA_v10020216mg
BRP : 103855428 103870561 103874572 117131528 117131533
BNA : 106352159 106378743 106427351 106433828 111215797 125587934
BOE : 106295759 106304855 106336393
RSZ : 108846505 108846508 108852523 108860069
THJ : 104816222 104823280 104827039
MINC : 123192666 123201994
GHI : 107898847 107899038 107905646 107941706 107958035
HSYR : 120137378 120156738 120183551 120202824
CCAJ : 109788592 109815064
APRC : 113848903 113860645
TPRA : 123888417 123924820
PSAT : 127100365 127127502
VVO : 131601283 131601299 131609350 131609363
LANG : 109339089 109340917
PCIN : 129288148 129290551
QSA : O6P43_016658 O6P43_026412
MSYL : 126592944 126619389
PXB : 103941742 103949448 103959590
CMAX : 111466182 111478187
CMOS : 111438145 111453716
MESC : 110620874 110622631
PALZ : 118037561 118037563 118048390
CILL : 122275567 122279926
ECAD : 122593609 122608141
CSIN : 114267699 114287661 114319874
AEW : 130783168 130794378 130795154
TDC : 119286366 119295370 119353000 119362063
TAES : 123043384 123051254 123086552 123091397 123096366 123187213
LPER : 127294073 127332547
MFLO : 136475943 136483702 136483737
PVIR : 120644093 120685815
ZOF : 121988461 121997344 122008648 122015066
CRE : CHLRE_08g376700v5 CHLRE_33g758897v5
SCE : YMR180C(CTL1) YPL228W(CET1)
SEUB : DI49_4263 DI49_5240
SPAO : SPAR_M02950 SPAR_P00480
CGR : 2886977(GVI51_D03575) 2887417(GVI51_E05753)
NCS : NCAS_0G01280(NCAS0G01280)
NDI : NDAI_0F01410(NDAI0F01410)
TPF : TPHA_0H01840(TPHA0H01840)
TBL : TBLA_0A02200(TBLA0A02200)
TDL : TDEL_0F01840(TDEL0F01840)
KAF : KAFR_0F01220(KAFR0F01220)
KNG : KNAG_0D00910(KNAG0D00910)
LBG : 92206194(LODBEIA_P09980)
CAL : CAALFM_CR02090CA(CET1)
YLI : 2908374(YALI2_F00826g)
CDEP : 91088259(L203_104049)
KMG : 30162271(I203_107709)
KNE : 92182943(IAR55_005685)
CCAC : CcaHIS019_0112080(CET1)
PPL : POSPLDRAFT_134921 POSPLDRAFT_134922
ADL : AURDEDRAFT_111235 AURDEDRAFT_112500
ABP : AGABI1DRAFT100277(AGABI1DRAFT_100277)
ABV : AGABI2DRAFT151998(AGABI2DRAFT_151998)
SCM : SCHCO_02616059(SCHCODRAFT_02616059)
PSTR : Pst134EA_003106 Pst134EA_003108
EHI : EHI_035610(17.t00048)
» show all
Taxonomy
Reference
Authors
Itoh N, Mizumoto K, Kaziro Y.
Title
Messenger RNA guanlyltransferase from Saccharomyces cerevisiae. II. Catalytic properties.
Journal
J Biol Chem 259:13930-6 (1984)
Reference
Authors
Tsukamoto T, Shibagaki Y, Murakoshi T, Suzuki M, Nakamura A, Gotoh H, Mizumoto K.
Title
Cloning and characterization of two human cDNAs encoding the mRNA capping enzyme.
Journal
Sequence
Reference
Authors
Vasiljeva L, Merits A, Auvinen P, Kaariainen L.
Title
Identification of a novel function of the alphavirus capping apparatus. RNA 5'-triphosphatase activity of Nsp2.
Journal
Other DBs
ExplorEnz - The Enzyme Database: 3.6.1.74
ExPASy - ENZYME nomenclature database: 3.6.1.74