KEGG   ENZYME: 3.6.1.74
Entry
EC 3.6.1.74                 Enzyme                                 

Name
mRNA 5'-phosphatase;
5'-polynucleotidase;
polynucleotide 5'-phosphohydrolase;
RNGTT (gene name);
CET1 (gene name);
mRNA 5'-triphosphate monophosphatase
Class
Hydrolases;
Acting on acid anhydrides;
In phosphorus-containing anhydrides
Sysname
5'-triphospho-mRNA 5'-phosphohydrolase
Reaction(IUBMB)
a 5'-triphospho-[mRNA] + H2O = a 5'-diphospho-[mRNA] + phosphate
Substrate
5'-triphospho-[mRNA] [CPD:C20864];
H2O [CPD:C00001]
Product
5'-diphospho-[mRNA] [CPD:C02100];
phosphate [CPD:C00009]
Comment
The enzyme, found in eukaryotes and some plus strand RNA viruses (e.g. alphavirus), is involved in mRNA capping. Unlike the eukaryotic enzyme, the viral enzyme requires a purine in the first position of the mRNA. The human enzyme is a multi domain protein that also has the activity of EC 2.7.7.50, mRNA guanylyltransferase.
History
EC 3.6.1.74 created 2021
Orthology
K01098  polynucleotide 5'-triphosphatase
K13917  mRNA-capping enzyme
Genes
HSA: 8732(RNGTT)
PTR: 462878(RNGTT)
PPS: 100993736(RNGTT)
GGO: 101136858(RNGTT)
PON: 100439097(RNGTT)
NLE: 100588336(RNGTT)
MCC: 702334(RNGTT)
MCF: 102125723(RNGTT)
CSAB: 103240079(RNGTT)
CATY: 105588991(RNGTT)
PANU: 101001398(RNGTT)
RRO: 104657908(RNGTT)
RBB: 108532637(RNGTT)
TFN: 117097068(RNGTT)
PTEH: 111538067(RNGTT)
CJC: 100394937(RNGTT)
SBQ: 101049020(RNGTT)
MMUR: 105863602(RNGTT)
MMU: 24018(Rngtt)
MCAL: 110292288(Rngtt)
MPAH: 110338665(Rngtt)
RNO: 313131(Rngtt)
MCOC: 116087980(Rngtt)
MUN: 110547371(Rngtt)
CGE: 100770876(Rngtt) 100773893
PLEU: 114681721 114695435(Rngtt)
NGI: 103733076(Rngtt)
HGL: 101697192(Rngtt)
OCU: 100340242(RNGTT)
OPI: 101535862 101536652(RNGTT)
TUP: 102495064(RNGTT)
CFA: 481914(RNGTT)
VVP: 112908388(RNGTT)
VLG: 121500237(RNGTT)
AML: 100483690(RNGTT)
UMR: 103659864(RNGTT)
UAH: 113264837(RNGTT)
ORO: 101370328(RNGTT)
ELK: 111158833
MPUF: 101690994(RNGTT)
EJU: 114213060(RNGTT)
MLX: 118001301(RNGTT)
FCA: 101096893(RNGTT)
PYU: 121029764(RNGTT)
PBG: 122489749(RNGTT)
PTG: 102959264(RNGTT)
PPAD: 109263391(RNGTT)
AJU: 106972542(RNGTT)
HHV: 120231871(RNGTT)
BTA: 511408(RNGTT)
BOM: 102269511(RNGTT)
BBUB: 102408950(RNGTT)
CHX: 102177341(RNGTT)
OAS: 101111376(RNGTT)
ODA: 120862736(RNGTT)
CCAD: 122422548(RNGTT)
SSC: 100520874(RNGTT)
CFR: 102516343(RNGTT)
CBAI: 105064127(RNGTT)
CDK: 105095541(RNGTT)
BACU: 103013048(RNGTT)
OOR: 101288281(RNGTT)
DLE: 111163638(RNGTT)
PCAD: 102995880(RNGTT)
ECB: 100065700(RNGTT)
EPZ: 103549521(RNGTT)
EAI: 106826688(RNGTT)
MYB: 102249374(RNGTT)
MYD: 102770454(RNGTT)
MMYO: 118659810(RNGTT)
MNA: 107533435(RNGTT)
PKL: 118705103(RNGTT)
DRO: 112296942(RNGTT)
SHON: 118982278(RNGTT)
AJM: 119038679(RNGTT)
MMF: 118621042(RNGTT)
PALE: 102887732(RNGTT)
PGIG: 120594956(RNGTT)
RAY: 107508669(RNGTT)
TOD: 119252683(RNGTT)
LAV: 100671236(RNGTT)
MDO: 100025582(RNGTT)
SHR: 100921795(RNGTT)
PCW: 110192035(RNGTT)
OAA: 100082839(RNGTT)
GGA: 421819(RNGTT)
PCOC: 116240660(RNGTT)
MGP: 100541550(RNGTT)
CJO: 107311978(RNGTT)
NMEL: 110395836(RNGTT)
ACYG: 106035397(RNGTT)
TGU: 100232080(RNGTT)
LSR: 110478776(RNGTT)
SCAN: 103819287(RNGTT)
PMOA: 120511792(RNGTT)
OTC: 121340035(RNGTT)
PRUF: 121360529(RNGTT)
GFR: 102031604(RNGTT)
FAB: 101811251(RNGTT)
PHI: 102104686(RNGTT)
PMAJ: 107201707(RNGTT)
CCW: 104694262(RNGTT)
ETL: 114063766(RNGTT)
FPG: 101910294(RNGTT)
FCH: 102049627(RNGTT)
CLV: 102089917
EGZ: 104135255(RNGTT)
NNI: 104021950(RNGTT)
ACUN: 113478600(RNGTT)
PADL: 103924648(RNGTT)
AAM: 106489707(RNGTT)
AROW: 112974970(RNGTT)
NPD: 112946564(RNGTT)
DNE: 112979324(RNGTT)
ASN: 102376947(RNGTT)
AMJ: 102570226(RNGTT)
CPOO: 109311853(RNGTT)
GGN: 109299876(RNGTT)
PSS: 102453098(RNGTT)
CMY: 102935462(RNGTT)
CPIC: 101947951(RNGTT)
TST: 117875114(RNGTT)
CABI: 116818018
ACS: 100560223(rngtt)
PVT: 110076717(RNGTT)
PBI: 103048279(RNGTT)
PMUR: 107296765(RNGTT)
PGUT: 117660756(RNGTT)
PMUA: 114593943(RNGTT)
ZVI: 118082264(RNGTT)
GJA: 107115389(RNGTT)
XLA: 399383(rngtt.S)
XTR: 407900(rngtt)
NPR: 108785315(RNGTT)
DRE: 405803(rngtt)
SRX: 107731501 107742988(rngtt)
SANH: 107660510 107680298(rngtt)
SGH: 107554925
IPU: 108256101(rngtt)
PHYP: 113529038(rngtt)
EEE: 113590000(rngtt)
TRU: 101079531(rngtt)
LCO: 104936194(rngtt)
CGOB: 115028774(rngtt)
ELY: 117270900(rngtt)
PLEP: 121955307(rngtt)
SLUC: 116066138(rngtt)
ECRA: 117961477(rngtt)
PFLV: 114573592(rngtt)
GAT: 120808521(rngtt)
PPUG: 119194746(rngtt)
MSAM: 119909597(rngtt)
CUD: 121520906(rngtt)
MZE: 101470505(rngtt)
ONL: 100693252(rngtt)
OAU: 116312742(rngtt)
OLA: 101173353(rngtt)
OML: 112158300(rngtt)
XMA: 102225982(rngtt)
XCO: 114158778(rngtt)
XHE: 116734325(rngtt)
PRET: 103457371(rngtt)
CVG: 107105147(rngtt)
CTUL: 119771567(rngtt)
NFU: 107377729(rngtt)
KMR: 108233334(rngtt)
ALIM: 106525679(rngtt)
CSEM: 103381224(rngtt)
LCF: 108873496(rngtt)
SDU: 111239258(rngtt)
SLAL: 111661520(rngtt)
XGL: 120789264(rngtt)
HCQ: 109508037(rngtt)
BPEC: 110173038(rngtt)
MALB: 109962341(rngtt)
SASA: 100380472(rngtt)
OTW: 112217500(rngtt)
OMY: 110522104(rngtt)
SALP: 111972809(rngtt)
SNH: 120019650(rngtt)
ELS: 105017860(rngtt)
SFM: 108929338(rngtt)
PKI: 111843049(rngtt)
AANG: 118230509(rngtt)
LOC: 102693195(rngtt)
PSPA: 121317600(rngtt)
ARUT: 117411619(rngtt)
LCM: 102363178(RNGTT)
CMK: 103186202(rngtt)
RTP: 109911996(rngtt)
BFO: 118404722
BBEL: 109465799
SCLV: 120337899
SPU: 577418
DME: Dmel_CG1810(mRNA-cap)
DER: 6550097
DSE: 6618519
DSI: Dsimw501_GD15855(Dsim_GD15855)
DAN: 6498788
DSR: 110180624
DPE: 6599294
DMN: 108155312
DWI: 6648910
DGR: 6565059
DAZ: 108619342
DNV: 108656840
DHE: 111596546
DVI: 6631663
CCAT: 101454832
BOD: 106616714
SCAC: 106087451
ACOZ: 120952170
AARA: 120898157
AAG: 5575290
AALB: 109431612
CPII: 120425624
AME: 414001
ACER: 107995092
BBIF: 117207087
BVK: 117237346
BVAN: 117160926
CCAL: 108622917
OBB: 114878850
MGEN: 117229853
NMEA: 116428246
CGIG: 122404724
PFUC: 122526774
TPRE: 106649390
FAS: 105269236
DAM: 107040204
CCIN: 107272598
TCA: 660879
AGB: 108908923
LDC: 111513901
APLN: 108740019
PPYR: 116172284
OTU: 111424689
MSEX: 115456279
BANY: 112053878
PPOT: 106099499
PXU: 106127656
HHAL: 106691572
NLU: 120350672
FOC: 113217495
CSEC: 111869117
HAME: 121879502
HAZT: 108675378
EAF: 111706330
DSV: 119450721
RSAN: 119383714
RMP: 119170759
VDE: 111250669
VJA: 111261602
SDM: 118185145
CEL: CELE_C03D6.3(cel-1)
CBR: CBG03761
BGT: 106051004
GAE: 121377111
PMAX: 117316469
OSN: 115223445
NVE: 5511796
ATEN: 116300710
HMG: 100205625
CPAP: 110814241
CIT: 102609768
PVY: 116134565
TCC: 18597604
EGR: 104426395
VRA: 106753467
VAR: 108322542
VUN: 114166056
LJA: Lj0g3v0256839.2(Lj0g3v0256839.2) Lj3g3v1603870.2(Lj3g3v1603870.2)
ADU: 107475134
AIP: 107625682
FVE: 101311588
RCN: 112193240
PPER: 18775434
PMUM: 103338253
PAVI: 110757331
PDUL: 117631546
ZJU: 107430745
CSV: 101209960
CMO: 103491007
BHJ: 120073197
MCHA: 111019426
CPEP: 111803494
RCU: 8262595
JCU: 105646640
POP: 18100001
PEU: 105121971
VVI: 100256199
VRI: 117920000
SLY: 101258480
SPEN: 107031176
SOT: 102584702
CANN: 107847477
NTA: 107818261
NSY: 104248191
NTO: 104100989
NAU: 109229421
SIND: 105161563
OEU: 111373839
LSV: 111884062
CCAV: 112507049
BVG: 104908854
SOE: 110804970
NNU: 104597018
MING: 122090070
PSOM: 113303508
DOSA: Os12t0193200-01(Os12g0193200)
SBI: 8071691
SITA: 101755911
PHAI: 112902508
MUS: 103992112
DCT: 110110603
PEQ: 110021775
AOF: 109832843
ATR: 18436476
PPP: 112283864
APRO: F751_1379
SCE: YMR180C(CTL1) YPL228W(CET1)
ERC: Ecym_2161
KMX: KLMA_70222(CET1)
NCS: NCAS_0G01280(NCAS0G01280)
NDI: NDAI_0F01410(NDAI0F01410)
TPF: TPHA_0H01840(TPHA0H01840)
TBL: TBLA_0A02200(TBLA0A02200)
TDL: TDEL_0F01840(TDEL0F01840)
KAF: KAFR_0F01220(KAFR0F01220)
CAL: CAALFM_CR02090CA(CET1)
CDU: CD36_27010(CET1)
SLB: AWJ20_3443(CET1)
PTE: PTT_11682
SPO: SPAC644.04(pct1)
CNE: CNF04680
CNB: CNBF0220
TASA: A1Q1_07765
ABP: AGABI1DRAFT100277(AGABI1DRAFT_100277)
ABV: AGABI2DRAFT151998(AGABI2DRAFT_151998)
MRT: MRET_3518
EHI: EHI_035610(17.t00048)
 » show all
Reference
1  [PMID:6094533]
  Authors
Itoh N, Mizumoto K, Kaziro Y.
  Title
Messenger RNA guanlyltransferase from Saccharomyces cerevisiae. II. Catalytic properties.
  Journal
J Biol Chem 259:13930-6 (1984)
Reference
2  [PMID:9473487]
  Authors
Tsukamoto T, Shibagaki Y, Murakoshi T, Suzuki M, Nakamura A, Gotoh H, Mizumoto K.
  Title
Cloning and characterization of two human cDNAs encoding the mRNA capping enzyme.
  Journal
Biochem Biophys Res Commun 243:101-8 (1998)
DOI:10.1006/bbrc.1997.8038
  Sequence
[hsa:8732]
Reference
3  [PMID:10748213]
  Authors
Vasiljeva L, Merits A, Auvinen P, Kaariainen L.
  Title
Identification of a novel function of the alphavirus capping apparatus. RNA 5'-triphosphatase activity of Nsp2.
  Journal
J Biol Chem 275:17281-7 (2000)
DOI:10.1074/jbc.M910340199
Other DBs
ExplorEnz - The Enzyme Database: 3.6.1.74
IUBMB Enzyme Nomenclature: 3.6.1.74
ExPASy - ENZYME nomenclature database: 3.6.1.74
BRENDA, the Enzyme Database: 3.6.1.74

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