Entry Name bile-acid 7alpha-dehydratase;
Class Lyases;BRITE hierarchy
Sysname 7alpha,12alpha-dihydroxy-3-oxochol-4-enoyl-CoA hydro-lyase (12alpha-hydroxy-3-oxochola-4,6-dienoyl-CoA-forming)
Reaction(IUBMB) 7alpha,12alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA = 12alpha-hydroxy-3-oxochola-4,6-dien-24-oyl-CoA + H2O [RN:
R11565 ]
Reaction(KEGG) Substrate 7alpha,12alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA [CPD:
C21469 ]
Product 12alpha-hydroxy-3-oxochola-4,6-dien-24-oyl-CoA [CPD:
C21470 ];
H2O [CPD:
C00001 ]
Comment This enzyme, characterized from the gut bacterium Clostridium scindens (previously known as Eubacterium sp. strain VPI 12708), participates in the 7-dehydroxylation process associated with bile acid degradation.
History EC 4.2.1.106 created 2005, modified 2016
Pathway ec00121 Secondary bile acid biosynthesis
Orthology K15872 bile-acid 7alpha-dehydratase
Reference Authors Mallonee DH, White WB, Hylemon PB
Title Cloning and sequencing of a bile acid-inducible operon from Eubacterium sp. strain VPI 12708.
Journal Reference Authors Dawson JA, Mallonee DH, Bjorkhem I, Hylemon PB
Title Expression and characterization of a C24 bile acid 7 alpha-dehydratase from Eubacterium sp. strain VPI 12708 in Escherichia coli.
Journal J Lipid Res 37:1258-67 (1996)
Sequence Reference Authors Bhowmik S, Chiu HP, Jones DH, Chiu HJ, Miller MD, Xu Q, Farr CL, Ridlon JM, Wells JE, Elsliger MA, Wilson IA, Hylemon PB, Lesley SA
Title Structure and functional characterization of a bile acid 7alpha dehydratase BaiE in secondary bile acid synthesis.
Journal Other DBs ExPASy - ENZYME nomenclature database: 4.2.1.106
