KEGG ENZYME: 4.2.1.144

KEGG

ENZYME: 4.2.1.144

Entry

EC 4.2.1.144                Enzyme

Name

3-amino-5-hydroxybenzoate synthase;
AHBA synthase;
rifK (gene name)

Class

Lyases;
Carbon-oxygen lyases;
Hydro-lyases

Sysname

5-amino-5-deoxy-3-dehydroshikimate hydro-lyase (3-amino-5-hydroxybenzoate-forming)

Reaction(IUBMB)

5-amino-5-deoxy-3-dehydroshikimate = 3-amino-5-hydroxybenzoate + H2O [RN:R06595]

Reaction(KEGG)

Substrate

5-amino-5-deoxy-3-dehydroshikimate [CPD:C12108]

Product

3-amino-5-hydroxybenzoate [CPD:C12107];
H2O [CPD:C00001]

Comment

A pyridoxal 5'-phosphate enzyme. The enzyme from the bacterium Amycolatopsis mediterranei participates in the pathway for rifamycin B biosynthesis. The enzyme also functions as a transaminase earlier in the pathway, producing UDP-alpha-D-kanosamine [3].

History

EC 4.2.1.144 created 2013

Pathway

Biosynthesis of ansamycins

Biosynthesis of secondary metabolites

Orthology

3-amino-5-hydroxybenzoate synthase

Genes

ROI:	N4261_13590

RBJ75_02255

AUB:	LXB15_06500

PSE45_14560

HP435_15095

JI735_17065

P0092_18725 P0092_18730

K5I26_02630

SVL:	Strvi_5368

SRC:	M271_45560

SMALA_0450 SMALA_1356

I1A49_05770 I1A49_10280(rifK)

SRW:	TUE45_00080(rifK)

SLE:	cxmK(cxmK)

Spa2297_32585

NFX46_22810

BV401_06010 BV401_10595

SVU:	B1H20_29920

BFF78_27075

F9278_17960

CP983_04310

CP982_35325 CP982_36640

S1361_01090(rifK1) S1361_34015(rifK2)

I6J39_29725

SDD:	D9753_04175 D9753_04745

LDH80_10615

QR300_08285

QR97_38425

YIA:	LO772_07395

KGD83_16365

FRA:	Francci3_0429

FAL:	FRAAL0914(gdnA)

FRE:	Franean1_1659 Franean1_4841

SEN:	SACE_2870(rifK)

AMD:	AMED_0627(rifK)

AMN:

RAM_03205

AMM:	AMES_0625(rifK)

AMZ:	B737_0626(rifK)

AAB:	A4R43_38525

YIM_39450(rifK)

SD37_10940 SD37_16800

CNX65_16265 CNX65_16520

AMI:	Amir_3196 Amir_3287

C1701_25910

SAQ:

Sare_1255

MAU:	Micau_2448

MIL:

ML5_5914

MicB006_3068

HXZ27_10305

GCM10017556_49860(rifK)

PVK74_10425

H1D33_20290

PVK37_09240

L083_2890 L083_5704

C6361_34460

C6W10_21740

PRY:	Prubr_62820(rifK)

GM418_28500

Reference

1 [PMID:9497318]

Authors

Kim CG, Yu TW, Fryhle CB, Handa S, Floss HG

Title

3-Amino-5-hydroxybenzoic acid synthase, the terminal enzyme in the formation of the precursor of mC7N units in rifamycin and related antibiotics.

Journal

J Biol Chem 273:6030-40 (1998)
DOI:10.1074/jbc.273.11.6030

Sequence

[amn:RAM_03205]

Reference

2 [PMID:10433690]

Authors

Eads JC, Beeby M, Scapin G, Yu TW, Floss HG

Title

Crystal structure of 3-amino-5-hydroxybenzoic acid (AHBA) synthase.

Journal

Biochemistry 38:9840-9 (1999)
DOI:10.1021/bi990018q

Sequence

[amn:RAM_03205]

Reference

3 [PMID:21081954]

Authors

Floss HG, Yu TW, Arakawa K

Title

The biosynthesis of 3-amino-5-hydroxybenzoic acid (AHBA), the precursor of mC7N units in ansamycin and mitomycin antibiotics: a review.

Journal

J Antibiot (Tokyo) 64:35-44 (2011)
DOI:10.1038/ja.2010.139

Sequence

[amn:RAM_03205]

Other DBs

ExplorEnz - The Enzyme Database:

IUBMB Enzyme Nomenclature:

ExPASy - ENZYME nomenclature database:

BRENDA, the Enzyme Database:

DBGET integrated database retrieval system