KEGG   ENZYME: 4.2.1.144Help
Entry
EC 4.2.1.144                Enzyme                                 

Name
3-amino-5-hydroxybenzoate synthase;
AHBA synthase;
rifK (gene name)
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
5-amino-5-deoxy-3-dehydroshikimate hydro-lyase (3-amino-5-hydroxybenzoate-forming)
Reaction(IUBMB)
5-amino-5-deoxy-3-dehydroshikimate = 3-amino-5-hydroxybenzoate + H2O [RN:R06595]
Reaction(KEGG)
Substrate
5-amino-5-deoxy-3-dehydroshikimate [CPD:C12108]
Product
3-amino-5-hydroxybenzoate [CPD:C12107];
H2O [CPD:C00001]
Comment
A pyridoxal 5'-phosphate enzyme. The enzyme from the bacterium Amycolatopsis mediterranei participates in the pathway for rifamycin B biosynthesis. The enzyme also functions as a transaminase earlier in the pathway, producing UDP-alpha-D-kanosamine [3].
History
EC 4.2.1.144 created 2013
Pathway
ec01051  Biosynthesis of ansamycins
ec01110  Biosynthesis of secondary metabolites
ec01130  Biosynthesis of antibiotics
Orthology
K16016  3-amino-5-hydroxybenzoate synthase
Genes
XDO: XDD1_2444
XPO: XPG1_2297
PSEC: CCOS191_4015(rifK)
PSOS: POS17_3680(ML5_5914)
BVR: BVIR_342
SIW: GH266_01095
BFM: BP422_10115
BAGR: BA6348_15085
PGM: PGRAT_08240
PRI: PRIO_1762
SRW: TUE45_00080(rifK)
SLE: cxmK(cxmK)
FAL: FRAAL0914(gdnA)
SEN: SACE_2870(rifK)
AMD: AMED_0627(rifK)
AMN: RAM_03205
AMM: AMES_0625(rifK)
AMZ: B737_0626(rifK)
AMYY: YIM_39450(rifK)
SAQ: Sare_1255
MIL: ML5_5914
 » show all
Taxonomy
Reference
1  [PMID:9497318]
  Authors
Kim CG, Yu TW, Fryhle CB, Handa S, Floss HG
  Title
3-Amino-5-hydroxybenzoic acid synthase, the terminal enzyme in the formation of the precursor of mC7N units in rifamycin and related antibiotics.
  Journal
J Biol Chem 273:6030-40 (1998)
DOI:10.1074/jbc.273.11.6030
  Sequence
[amn:RAM_03205]
Reference
2  [PMID:10433690]
  Authors
Eads JC, Beeby M, Scapin G, Yu TW, Floss HG
  Title
Crystal structure of 3-amino-5-hydroxybenzoic acid (AHBA) synthase.
  Journal
Biochemistry 38:9840-9 (1999)
DOI:10.1021/bi990018q
  Sequence
[amn:RAM_03205]
Reference
3  [PMID:21081954]
  Authors
Floss HG, Yu TW, Arakawa K
  Title
The biosynthesis of 3-amino-5-hydroxybenzoic acid (AHBA), the precursor of mC7N units in ansamycin and mitomycin antibiotics: a review.
  Journal
J Antibiot (Tokyo) 64:35-44 (2011)
DOI:10.1038/ja.2010.139
  Sequence
[amn:RAM_03205]
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.144
IUBMB Enzyme Nomenclature: 4.2.1.144
ExPASy - ENZYME nomenclature database: 4.2.1.144
BRENDA, the Enzyme Database: 4.2.1.144

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