KEGG   ENZYME: 4.2.1.144
Entry
EC 4.2.1.144                Enzyme                                 
Name
3-amino-5-hydroxybenzoate synthase;
AHBA synthase;
rifK (gene name)
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
Sysname
5-amino-5-deoxy-3-dehydroshikimate hydro-lyase (3-amino-5-hydroxybenzoate-forming)
Reaction(IUBMB)
5-amino-5-deoxy-3-dehydroshikimate = 3-amino-5-hydroxybenzoate + H2O [RN:R06595]
Reaction(KEGG)
R06595
Substrate
5-amino-5-deoxy-3-dehydroshikimate [CPD:C12108]
Product
3-amino-5-hydroxybenzoate [CPD:C12107];
H2O [CPD:C00001]
Comment
A pyridoxal 5'-phosphate enzyme. The enzyme from the bacterium Amycolatopsis mediterranei participates in the pathway for rifamycin B biosynthesis. The enzyme also functions as a transaminase earlier in the pathway, producing UDP-alpha-D-kanosamine [3].
History
EC 4.2.1.144 created 2013
Pathway
ec01051  Biosynthesis of ansamycins
ec01110  Biosynthesis of secondary metabolites
Orthology
K16016  3-amino-5-hydroxybenzoate synthase
Genes
AUBLXB15_06500
PSONJI735_17065
SINTK5I26_02630
SVLStrvi_5368
SRCM271_45560
SMALSMALA_0450 SMALA_1356
SSOII1A49_05770 I1A49_10280(rifK)
SRWTUE45_00080(rifK)
SLEcxmK(cxmK)
SPAVSpa2297_32585
SPHWNFX46_22810
SAUOBV401_06010 BV401_10595
SVUB1H20_29920
SPUNBFF78_27075
SPHVF9278_17960
SCHACP983_04310
SSPBCP982_35325 CP982_36640
SCYGS1361_01090(rifK1) S1361_34015(rifK2)
SCALI6J39_29725
SDDD9753_04175 D9753_04745
YIALO772_07395
FRAFrancci3_0429
FREFranean1_1659 Franean1_4841
FALFRAAL0914(gdnA)
SENSACE_2870(rifK)
AMDAMED_0627(rifK)
AMNRAM_03205
AMMAMES_0625(rifK)
AMZB737_0626(rifK)
AABA4R43_38525
AMYYYIM_39450(rifK)
AORISD37_10940 SD37_16800
APRECNX65_16265 CNX65_16520
AMIAmir_3196 Amir_3287
ACTAC1701_25910
SAQSare_1255
MAUMicau_2448
MILML5_5914
MICBMicB006_3068
MCABHXZ27_10305
MSAGGCM10017556_49860(rifK)
ACTNL083_2890 L083_5704
PLABC6361_34460
PLATC6W10_21740
PRYPrubr_62820(rifK)
MCOSGM418_28500
 » show all
Reference
1  [PMID:9497318]
  Authors
Kim CG, Yu TW, Fryhle CB, Handa S, Floss HG
  Title
3-Amino-5-hydroxybenzoic acid synthase, the terminal enzyme in the formation of the precursor of mC7N units in rifamycin and related antibiotics.
  Journal
J Biol Chem 273:6030-40 (1998)
DOI:10.1074/jbc.273.11.6030
  Sequence
[amn:RAM_03205]
Reference
2  [PMID:10433690]
  Authors
Eads JC, Beeby M, Scapin G, Yu TW, Floss HG
  Title
Crystal structure of 3-amino-5-hydroxybenzoic acid (AHBA) synthase.
  Journal
Biochemistry 38:9840-9 (1999)
DOI:10.1021/bi990018q
  Sequence
[amn:RAM_03205]
Reference
3  [PMID:21081954]
  Authors
Floss HG, Yu TW, Arakawa K
  Title
The biosynthesis of 3-amino-5-hydroxybenzoic acid (AHBA), the precursor of mC7N units in ansamycin and mitomycin antibiotics: a review.
  Journal
J Antibiot (Tokyo) 64:35-44 (2011)
DOI:10.1038/ja.2010.139
  Sequence
[amn:RAM_03205]
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.144
IUBMB Enzyme Nomenclature: 4.2.1.144
ExPASy - ENZYME nomenclature database: 4.2.1.144
BRENDA, the Enzyme Database: 4.2.1.144

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