| Entry |
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| Name |
germacrene-A synthase;
germacrene A synthase;
(+)-germacrene A synthase;
(+)-(10R)-germacrene A synthase;
GAS;
2-trans,6-trans-farnesyl-diphosphate diphosphate-lyase (germacrene-A-forming)
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| Class |
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
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| Sysname |
(2E,6E)-farnesyl-diphosphate diphosphate-lyase [(+)-(R)-germacrene-A-forming]
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| Reaction(IUBMB) |
(2E,6E)-farnesyl diphosphate = (+)-(R)-gemacrene A + diphosphate [RN: R07649]
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| Reaction(KEGG) |
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| Substrate |
(2E,6E)-farnesyl diphosphate [CPD: C00448]
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| Product |
(+)-(R)-gemacrene A;
diphosphate [CPD: C00013]
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| Comment |
Requires Mg2+ for activity. While germacrene A is an enzyme-bound intermediate in the biosynthesis of a number of phytoalexins, e.g. EC 4.2.3.9 (aristolochene synthase) from some species and EC 4.2.3.21 (vetispiradiene synthase), it is the sole sesquiterpenoid product formed in chicory [1].
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| History |
EC 4.2.3.23 created 2006
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| Pathway |
| ec00909 | Sesquiterpenoid and triterpenoid biosynthesis |
| ec01110 | Biosynthesis of secondary metabolites |
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| Orthology |
| K21927 | alpha-muurolene/germacrene-A/gamma-muurolene synthase |
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| Genes |
» show all
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| Reference |
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| Authors |
Bouwmeester HJ, Kodde J, Verstappen FW, Altug IG, de Kraker JW, Wallaart TE |
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| Title |
Isolation and characterization of two germacrene A synthase cDNA clones from chicory. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Prosser I, Phillips AL, Gittings S, Lewis MJ, Hooper AM, Pickett JA, Beale MH. |
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| Title |
(+)-(10R)-Germacrene A synthase from goldenrod, Solidago canadensis; cDNA isolation, bacterial expression and functional analysis. |
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| Journal |
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| Reference |
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| Authors |
de Kraker JW, Franssen MC, de Groot A, Konig WA, Bouwmeester HJ |
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| Title |
(+)-Germacrene A biosynthesis . The committed step in the biosynthesis of bitter sesquiterpene lactones in chicory |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Calvert MJ, Ashton PR, Allemann RK. |
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| Title |
Germacrene A is a product of the aristolochene synthase-mediated conversion of farnesylpyrophosphate to aristolochene. |
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| Journal |
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| Reference |
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| Authors |
Chang YJ, Jin J, Nam HY, Kim SU. |
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| Title |
Point mutation of (+)-germacrene A synthase from Ixeris dentata. |
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| Journal |
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| Other DBs |
| ExplorEnz - The Enzyme Database: | 4.2.3.23 |
| ExPASy - ENZYME nomenclature database: | 4.2.3.23 |
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