Entry
Name
E1 NEDD8-activating enzyme;
NEDD-activating enzyme E1;
NAE1 (gene name);
UBA3 (gene name)
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
BRITE hierarchy
Sysname
[NEDD8 protein]:[E1 NEDD8-activating enzyme] ligase (AMP-forming)
Reaction(IUBMB)
ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine = AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine
Substrate
ATP [CPD:
C00002 ];
[NEDD8 protein];
[E1 NEDD8-activating enzyme]-L-cysteine
Product
AMP [CPD:
C00020 ];
diphosphate [CPD:
C00013 ];
[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine
Comment
Some RING-type E3 ubiquitin transferase (EC
2.3.2.27 ) are not able to bind a substrate protein directly. Instead, they form complexes with a cullin scaffold protein and a substrate recognition module, which are known as CRL (Cullin-RING-Ligase) complexes. The cullin protein needs to be activated by the ubiquitin-like protein NEDD8 in a process known as neddylation. Like ubiquitin, the NEDD8 protein ends with two glycine residues. The E1 NEDD8-activating enzyme activates NEDD8 in an ATP-dependent reaction by forming a high-energy thioester intermediate between NEDD8 and one of its cysteine residues. The activated NEDD8 is subsequently transferred to a cysteine residue of EC
2.3.2.34 , E2 NEDD8-conjugating enzyme, and is eventually conjugated to a lysine residue of specific substrates in the presence of the appropriate E3 transferase (EC
2.3.2.32 , cullin-RING-type E3 NEDD8 transferase).
History
EC 6.2.1.64 created 2020
Orthology
K10686 NEDD8-activating enzyme E1
Genes
CIMI : 108283730 108314065(UBA3)
ASYL : 127678977(Uba3) 127682340
NVS : 122898856 122899207 122899214 122909279(UBA3) 122913945
TOD : 119230920(UBA3) 119244539
ASAO : 132769411(UBA3) 132775615
XLA : 108714725(uba3.L) 734782(uba3.S)
SRX : 107729399 107737645 107753775
SANH : 107656056 107672020 107696817
SGH : 107558885 107580149 107600288
CCAR : 109061212(uba3) 109061224 109077613
CAUA : 113055001 113069100 113111027
CGIB : 127968206 128012157 128022425
PTET : 122329179 122354265(uba3)
LROH : 127155243 127173545(uba3)
GRF : 141290997 141294096(uba3)
OMC : 131532426 131549013(uba3)
MASI : 127426278 127428228
CMAO : 118799796(uba3) 118822616
CHAR : 105906787 105912256(uba3)
SPIL : 134088065 134092813(uba3)
NCC : 104944983 104955224(uba3)
TBEN : 117475652(uba3) 117479473
CGOB : 115008263 115011383
PGEO : 117446651(uba3) 117450116
GACU : 117543134 117543692(uba3)
EMAC : 134866439 134883547(uba3)
ELY : 117256264(uba3) 117258904
EFO : 125891409 125893796(uba3)
PLEP : 121946477 121949671(uba3)
SLUC : 116035189(uba3) 116061992
ECRA : 117943434(uba3) 117947055
PFLV : 114554110 114558205
GAT : 120828986 120835094(uba3)
PPUG : 119194095(uba3) 119222001
AFB : 129099610 129105657(uba3)
CLUM : 117731384(uba3) 117733286
PSWI : 130199322 130207973(uba3)
CANA : 137796302(uba3) 137800445
CVE : 137868438 137879627(uba3)
MSAM : 119886654(uba3) 119906123
SCHU : 122865348(uba3) 122883402
CUD : 121506998(uba3) 121516911
SPUL : 138589546 138605811(uba3)
LMIX : 132977617(uba3) 132989672
ALAT : 119021901(uba3) 119022861
OAU : 116310945 116330178(uba3)
OML : 112145215(uba3) 112158859
XMA : 102225974 102230113(uba3)
PRET : 103465053 103467665
PFOR : 103144012 103152910
PLAI : 106934653 106953339
PMEI : 106903451 106907052
GAF : 122832545 122834364(uba3)
PPRL : 129358396(uba3) 129370412
CTUL : 119782023 119789537
GMU : 124856575(uba3) 124870880
NFU : 107385429(uba3) 107396417
KMR : 108231938 108241173(uba3)
ALIM : 106518515(uba3) 106519301
NWH : 119411712(uba3) 119416830
AOCE : 111572109 111588779(uba3)
EJA : 138193352 138197832(uba3)
BFRE : 138624312 138629250(uba3)
MCEP : 125005552 125007431(uba3)
SSEN : 122768622 122777873
SSOE : 131461539(uba3) 131469229
HHIP : 117761467(uba3) 117764866
HSP : 118105365(uba3) 118111501
PPLT : 128442919 128459633(uba3)
SMAU : 118309923(uba3) 118317018
SLAL : 111655022 111670887
XGL : 120783499(uba3) 120803892
MALB : 109955907 109966755
BSPL : 114856283(uba3) 114859396
SJO : 128355467(uba3) 128357016
SSCO : 133977318(uba3) 133987454
SASA : 100196693(uba3) 106567275(uba3) 106572411
STRU : 115150783 115168117(uba3)
OGO : 124031431 124032034 124045894
OKE : 118359856 118360401 118400157
OMM : 135528233 135542086 135559330
OCLA : 139368596 139413403 139416640
SALP : 111966608 111969932 111976991
SNH : 120058867 120061175 120065095
CCLU : 121538490 121545638 121578473
OEP : 134018967 134026717(uba3)
SFM : 108923369(uba3) 108940090
PSPA : 121297887 121329119
ARUT : 117411901 117413772
DSI : Dsimw501_GD10991(Dsim_GD10991)
CLON : 129610295 129617884
CQU : CpipJ_CPIJ015388 CpipJ_CPIJ016052
SGRE : 126293670 126318312
IEL : 124154708 124155380 124171480
HAMR : 139686146 139687559 139694044(Uba3)
AFRA : 136042609 136042674
HAZT : 108673009 108677282
PPOI : 119107499 119108610
CBR : CBG_16503(Cbr-rfl-1)
BMY : BM_BM3316(Bma-rfl-1)
MMER : 123530526 128547822
MCAZ : 138018444 138019495
CSAT : 104706240 104735980 104770273
BNA : 106386769 106433658 106434242 111206274
HSYR : 120175254 120191703
LANG : 109329614 109329616 109337793
QSA : O6P43_003417 O6P43_021610
MSYL : 126596448 126608648
PCOX : 137731514 137737014
MESC : 110601552 110611230
PALZ : 118029704 118061045 118062744
SSPL : 121783630 121797418 121799575 121808475
SHIS : 125197539 125223467
PHUA : 140979001 140981151
AEW : 130763986 130764804 130775061
PSOM : 113336889 113357346
TAES : 123061091 123069678 123078164
TUA : 125543715 125543716 125543717
MFLO : 136449819 136488111
PVIR : 120673559 120706510
PAUA : 133899374 133909925
SMO : SELMODRAFT_106904 SELMODRAFT_174709
CGR : 2887237(GVI51_D01419)
NCS : NCAS_0A11140(NCAS0A11140)
NDI : NDAI_0A05160(NDAI0A05160)
TPF : TPHA_0A03600(TPHA0A03600)
TBL : TBLA_0H01590(TBLA0H01590)
TDL : TDEL_0C04980(TDEL0C04980)
KAF : KAFR_0H00930(KAFR0H00930)
KNG : KNAG_0H00670(KNAG0H00670)
LBG : 92209529(LODBEIA_P43330)
CAL : CAALFM_C600500CA(CaO19.4209)
ASAU : 88173358(PUMCH_002293)
YLI : 2907705(YALI2_F00908g)
NTE : NEUTE1DRAFT133618(NEUTE1DRAFT_133618)
SMP : 10803663(SMAC4_05822)
RTHE : 98123334(VTJ83DRAFT_2421)
TATV : 90959490(TrAtP1_004370)
TASP : 90966413(TrAFT101_003835)
PLJ : 28887349(PLICBS_002608)
CDET : 87939119(CDEST_02616)
CPW : 9697129(D8B26_003958)
PTRR : 6346239(PtrM4_136750)
ADAC : 96087234(ACET3X_006912)
CDEC : 89988119(IAS62_001344)
CTEG : 91988728(I308_101870)
CBAS : 91981919(I312_101871)
CDEP : 91089221(L203_105012)
KMG : 30165680(I203_100261)
KNE : 92181845(IAR55_004587)
KDJ : 28971652(I303_107960)
KBI : 30205654(I302_102568)
KPIN : 30175799(I206_106681)
KSH : 87959974(IL334_007844)
KER : 91103991(V865_005190)
CCAC : CcaHIS019_0200870(uba3)
ABP : AGABI1DRAFT111125(AGABI1DRAFT_111125)
ABV : AGABI2DRAFT190343(AGABI2DRAFT_190343)
SCM : SCHCO_02607608(SCHCODRAFT_02607608)
PGR : PGTG_00608 PGTG_17926 PGTG_20639
PSTR : Pst134EA_000626 Pst134EA_030431
EHI : EHI_098550(19.t00019)
PLAG : 39741383(PADL01_0816200)
PTI : PHATRDRAFT_33774(UBA3)
TVA : 4770259(TVAGG3_0228020) 4771601(TVAGG3_0330060)
SSAO : 94297665(SS50377_23642)
EHX : EMIHUDRAFT_425916 EMIHUDRAFT_428127
LOKI : Lokiarch_29320(moeB_2)
» show all
Taxonomy
Reference
Authors
Osaka F, Kawasaki H, Aida N, Saeki M, Chiba T, Kawashima S, Tanaka K, Kato S
Title
A new NEDD8-ligating system for cullin-4A.
Journal
Sequence
Reference
Authors
Gong L, Yeh ET
Title
Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 6.2.1.64
ExPASy - ENZYME nomenclature database: 6.2.1.64