KEGG   ENZYME: 3.5.2.18Help
Entry
EC 3.5.2.18                 Enzyme                                 

Name
enamidase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In cyclic amides
BRITE hierarchy
Sysname
6-oxo-1,4,5,6-tetrahydronicotinate amidohydrolase
Reaction(IUBMB)
6-oxo-1,4,5,6-tetrahydronicotinate + 2 H2O = 2-formylglutarate + NH3 [RN:R07984]
Reaction(KEGG)
Substrate
6-oxo-1,4,5,6-tetrahydronicotinate [CPD:C04226];
H2O [CPD:C00001]
Product
2-formylglutarate [CPD:C16159];
NH3 [CPD:C00014]
Comment
Contains iron and Zn2+. Forms part of the nicotinate-fermentation catabolism pathway in Eubacterium barkeri. Other enzymes involved in this pathway are EC 1.17.1.5 (nicotinate dehydrogenase), EC 1.3.7.1 (6-hydroxynicotinate reductase), EC 1.1.1.291 (2-hydroxymethylglutarate dehydrogenase), EC 5.4.99.4 (2-methyleneglutarate mutase), EC 5.3.3.6 (methylitaconate Delta-isomerase), EC 4.2.1.85 (dimethylmaleate hydratase) and EC 4.1.3.32 (2,3-dimethylmalate lyase).
History
EC 3.5.2.18 created 2006
Pathway
ec00760  Nicotinate and nicotinamide metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K15358  enamidase
Genes
GAI: IMCC3135_06255(ena)
BUG: BC1001_4282
BGF: BC1003_5118
BUQ: AC233_28060
BUZ: AYM40_31820
BXE: Bxe_B0661
BXB: DR64_5992(ena)
BPX: BUPH_00866
BPY: Bphyt_4330
BFN: OI25_6348
BTRM: SAMEA390648703552(Ena)
POL: Bpro_4072
VEI: Veis_0850
BJA: bll3830
BRA: BRADO2124
BBT: BBta_2433
BRS: S23_44100
AOL: S58_54100
BRAD: BF49_1213
RPB: RPB_1671
XAU: Xaut_4206
AZC: AZC_2804
HDI: HDIA_4511(Ena)
SIL: SPO0819
RDE: RD1_1529
OTM: OSB_05860
GXL: H845_1049
MAG: amb1486
CPAT: CLPA_c02940(ena)
CPAE: CPAST_c02940(ena)
TAE: TepiRe1_0451(Ena) TepiRe1_0461(Ena)
MVQ: MYVA_2367
SAQ: Sare_0577
CWO: Cwoe_3945
TRO: trd_A0335
 » show all
Taxonomy
Reference
1  [PMID:16894175]
  Authors
Alhapel A, Darley DJ, Wagener N, Eckel E, Elsner N, Pierik AJ.
  Title
Molecular and functional analysis of nicotinate catabolism in Eubacterium barkeri.
  Journal
Proc Natl Acad Sci U S A 103:12341-6 (2006)
DOI:10.1073/pnas.0601635103
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.5.2.18
IUBMB Enzyme Nomenclature: 3.5.2.18
ExPASy - ENZYME nomenclature database: 3.5.2.18
BRENDA, the Enzyme Database: 3.5.2.18

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