ID A0A093RR32_PYGAD Unreviewed; 352 AA.
AC A0A093RR32;
DT 26-NOV-2014, integrated into UniProtKB/TrEMBL.
DT 26-NOV-2014, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE RecName: Full=Lactosylceramide 4-alpha-galactosyltransferase {ECO:0000256|ARBA:ARBA00040835};
DE EC=2.4.1.228 {ECO:0000256|ARBA:ARBA00039051};
DE AltName: Full=Alpha-1,4-N-acetylglucosaminyltransferase {ECO:0000256|ARBA:ARBA00043186};
DE AltName: Full=Alpha-1,4-galactosyltransferase {ECO:0000256|ARBA:ARBA00041849};
DE AltName: Full=Globotriaosylceramide synthase {ECO:0000256|ARBA:ARBA00043154};
DE AltName: Full=UDP-galactose:beta-D-galactosyl-beta1-R 4-alpha-D-galactosyltransferase {ECO:0000256|ARBA:ARBA00041556};
DE Flags: Fragment;
GN ORFNames=AS28_08997 {ECO:0000313|EMBL:KFW73304.1};
OS Pygoscelis adeliae (Adelie penguin).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Sphenisciformes; Spheniscidae; Pygoscelis.
OX NCBI_TaxID=9238 {ECO:0000313|EMBL:KFW73304.1, ECO:0000313|Proteomes:UP000054081};
RN [1] {ECO:0000313|EMBL:KFW73304.1, ECO:0000313|Proteomes:UP000054081}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BGI_AS28 {ECO:0000313|EMBL:KFW73304.1};
RA Zhang G., Li C.;
RT "Genome evolution of avian class.";
RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-Cer(d18:1(4E)) + UDP-
CC alpha-D-galactose = a globoside Gb3Cer (d18:1(4E)) + H(+) + UDP;
CC Xref=Rhea:RHEA:11924, ChEBI:CHEBI:15378, ChEBI:CHEBI:17950,
CC ChEBI:CHEBI:18313, ChEBI:CHEBI:58223, ChEBI:CHEBI:66914;
CC EC=2.4.1.228; Evidence={ECO:0000256|ARBA:ARBA00043770};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:11925;
CC Evidence={ECO:0000256|ARBA:ARBA00043770};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=beta-D-Gal-(1<->1')-Cer + UDP-alpha-D-galactose = alpha-D-Gal-
CC (1->4)-beta-D-Gal-(1<->1')-Cer + H(+) + UDP; Xref=Rhea:RHEA:60044,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58223, ChEBI:CHEBI:66914,
CC ChEBI:CHEBI:143593, ChEBI:CHEBI:143594;
CC Evidence={ECO:0000256|ARBA:ARBA00036489};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60045;
CC Evidence={ECO:0000256|ARBA:ARBA00036489};
CC -!- PATHWAY: Glycolipid biosynthesis. {ECO:0000256|ARBA:ARBA00004934}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000256|ARBA:ARBA00004323}; Single-pass type II membrane protein
CC {ECO:0000256|ARBA:ARBA00004323}. Membrane
CC {ECO:0000256|ARBA:ARBA00004606}; Single-pass type II membrane protein
CC {ECO:0000256|ARBA:ARBA00004606}.
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DR EMBL; KL225530; KFW73304.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A093RR32; -.
DR STRING; 9238.A0A093RR32; -.
DR Proteomes; UP000054081; Unassembled WGS sequence.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016758; F:hexosyltransferase activity; IEA:UniProt.
DR GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR Gene3D; 3.90.550.20; -; 1.
DR InterPro; IPR007652; A1-4-GlycosylTfrase_dom.
DR InterPro; IPR007577; GlycoTrfase_DXD_sugar-bd_CS.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR PANTHER; PTHR12042:SF17; LACTOSYLCERAMIDE 4-ALPHA-GALACTOSYLTRANSFERASE; 1.
DR PANTHER; PTHR12042; LACTOSYLCERAMIDE 4-ALPHA-GALACTOSYLTRANSFERASE ALPHA- 1,4-GALACTOSYLTRANSFERASE; 1.
DR Pfam; PF04572; Gb3_synth; 1.
DR Pfam; PF04488; Gly_transf_sug; 1.
DR SUPFAM; SSF53448; Nucleotide-diphospho-sugar transferases; 1.
PE 4: Predicted;
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676,
KW ECO:0000313|EMBL:KFW73304.1};
KW Golgi apparatus {ECO:0000256|ARBA:ARBA00023034};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000054081};
KW Transferase {ECO:0000313|EMBL:KFW73304.1};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 20..38
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 223..348
FT /note="Alpha 1,4-glycosyltransferase"
FT /evidence="ECO:0000259|Pfam:PF04572"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:KFW73304.1"
FT NON_TER 352
FT /evidence="ECO:0000313|EMBL:KFW73304.1"
SQ SEQUENCE 352 AA; 39622 MW; 87C8E0D855D6D5DC CRC64;
RMSSCLPNLA TVVPSHGLRA LFILTTSFVF FAAVTFYWRT GEDAEGQLYS LPTQSRCAQF
LPSPPSAVAG GPPPPPGDVF FVETSERTNP SYLFTCSVES AARMHPGTRV VVLMKGLANG
NASLPNHWAF SMLSCFPNVE IQPLDLPELF SGTPLAKWYL QSEHQKEPYF LPVLSDACRI
AIMWKFGGIY LDTDFIVLRN LKNLTNALGI ECKYLLNGAF LSFKPKHKFM ELCMQDFVDN
YNGLIWGHQG PHLLTRVFKK WCSISNVQDN MSCKGVRALS PEAFYPICWE NWNKLFDTIS
SLELHKLLKN TYAVHVWNKL SHGTRLEITS QALLAQLYSQ FCPATCAKMK KD
//
