ID A0A1I8M1B8_MUSDO Unreviewed; 1034 AA.
AC A0A1I8M1B8;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 24-JAN-2024, entry version 40.
DE RecName: Full=nitric-oxide synthase (NADPH) {ECO:0000256|ARBA:ARBA00012989};
DE EC=1.14.13.39 {ECO:0000256|ARBA:ARBA00012989};
OS Musca domestica (House fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Muscoidea;
OC Muscidae; Musca.
OX NCBI_TaxID=7370 {ECO:0000313|EnsemblMetazoa:MDOA000248-PA};
RN [1] {ECO:0000313|EnsemblMetazoa:MDOA000248-PA}
RP IDENTIFICATION.
RC STRAIN=Aabys {ECO:0000313|EnsemblMetazoa:MDOA000248-PA};
RG EnsemblMetazoa;
RL Submitted (MAY-2020) to UniProtKB.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000256|ARBA:ARBA00001917};
CC -!- COFACTOR:
CC Name=heme b; Xref=ChEBI:CHEBI:60344;
CC Evidence={ECO:0000256|ARBA:ARBA00001970};
CC -!- SIMILARITY: Belongs to the NOS family. {ECO:0000256|ARBA:ARBA00006267}.
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DR AlphaFoldDB; A0A1I8M1B8; -.
DR STRING; 7370.A0A1I8M1B8; -.
DR EnsemblMetazoa; MDOA000248-RA; MDOA000248-PA; MDOA000248.
DR VEuPathDB; VectorBase:MDOA000248; -.
DR eggNOG; KOG1158; Eukaryota.
DR Proteomes; UP000095301; Unassembled WGS sequence.
DR GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR GO; GO:0004517; F:nitric-oxide synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0006809; P:nitric oxide biosynthetic process; IEA:InterPro.
DR Gene3D; 3.40.50.360; -; 1.
DR Gene3D; 3.90.440.10; Nitric Oxide Synthase;Heme Domain;Chain A domain 2; 1.
DR Gene3D; 3.40.50.80; Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module; 1.
DR Gene3D; 2.40.30.10; Translation factors; 1.
DR InterPro; IPR003097; CysJ-like_FAD-binding.
DR InterPro; IPR017927; FAD-bd_FR_type.
DR InterPro; IPR001094; Flavdoxin-like.
DR InterPro; IPR008254; Flavodoxin/NO_synth.
DR InterPro; IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
DR InterPro; IPR029039; Flavoprotein-like_sf.
DR InterPro; IPR039261; FNR_nucleotide-bd.
DR InterPro; IPR023173; NADPH_Cyt_P450_Rdtase_alpha.
DR InterPro; IPR044943; NOS_dom_1.
DR InterPro; IPR044940; NOS_dom_2.
DR InterPro; IPR044944; NOS_dom_3.
DR InterPro; IPR012144; NOS_euk.
DR InterPro; IPR004030; NOS_N.
DR InterPro; IPR036119; NOS_N_sf.
DR InterPro; IPR001433; OxRdtase_FAD/NAD-bd.
DR InterPro; IPR017938; Riboflavin_synthase-like_b-brl.
DR PANTHER; PTHR43410; NITRIC OXIDE SYNTHASE OXYGENASE; 1.
DR PANTHER; PTHR43410:SF1; NITRIC OXIDE SYNTHASE OXYGENASE; 1.
DR Pfam; PF00667; FAD_binding_1; 1.
DR Pfam; PF00258; Flavodoxin_1; 1.
DR Pfam; PF00175; NAD_binding_1; 1.
DR Pfam; PF02898; NO_synthase; 1.
DR PIRSF; PIRSF000333; NOS; 1.
DR PRINTS; PR00369; FLAVODOXIN.
DR PRINTS; PR00371; FPNCR.
DR SUPFAM; SSF52343; Ferredoxin reductase-like, C-terminal NADP-linked domain; 1.
DR SUPFAM; SSF52218; Flavoproteins; 1.
DR SUPFAM; SSF56512; Nitric oxide (NO) synthase oxygenase domain; 1.
DR SUPFAM; SSF63380; Riboflavin synthase domain-like; 1.
DR PROSITE; PS51384; FAD_FR; 1.
DR PROSITE; PS50902; FLAVODOXIN_LIKE; 1.
PE 3: Inferred from homology;
KW Calmodulin-binding {ECO:0000256|ARBA:ARBA00022860};
KW FAD {ECO:0000256|ARBA:ARBA00022827};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW FMN {ECO:0000256|ARBA:ARBA00022643}; Heme {ECO:0000256|ARBA:ARBA00022617};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW NADP {ECO:0000256|ARBA:ARBA00022857};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002}.
SQ SEQUENCE 1034 AA; 117880 MW; 5A50FC4ED75773CF CRC64;
MHKFLQIGNE FYFLFGFSFR FLQVFDCRYV TTTSGMFEAI CNHIKYATNK GNLRSAITIF
PQRTDARHDY RIWNSQLISY AGYKQPDGKI IGDPLNAEFT EVCLKLGWKG KGTEWDILPL
VVSANGHDPD YFDYPPELIL EVPLSHPKFE WFGEMGLRWY ALPAVSGMLF DVGGIQFTAT
AFSGWYMSTE IGCRNLCDVN RRNILETVAL KMNLDTRTPT SLWKDKALVE VNIAVLHSFQ
SRNVTIVDHH TASESFMKHF ENESKLRNGC PADWIWIVPP MSASITPVFH QEMSLYYLRP
SFEYQDPAWR THVWKKGRGE GKSKKPRRKF NFKQIARAVK FTSKLFGRAL SKRIKATVLY
ATETGKSEQY AKQLCELLGH AFNAQLYCMA DYDVSSIEHE ALLIVVASTF GNGDPPENGE
LFSQDLYAMR VQESGESGQD SSIVTSSKSF MKASSRQDFI KLPLQQVKKI DRWDSLRGST
SDTFTEENFG PLSNVRFAVF ALGSSAYPNF CAFGQYIDNV LGELGGERLL KVTYGDEMCG
QEQSFRKWAP EVFKVACETF CLDPDESLSE ASMALQNDSL TVNTVRLVPA KTKGSMEQLL
SKYHNKKVTC CQIKAHPHNL TNSGNGEKTT ILLEISAPGI DYEPGDHVGI FPSNRPEIVE
GLLRRLTGFE DPDELLQLQL LKEKQTSNGI FKCWESHDKI PAESMRTLLT KFFDVTTPPS
RQMLTLLSGF CEDNHDTERL QLLVNDSSAY EDWRHWRLPH LLDVLEEFPS CKPPASLVLA
NLMPLQSRFY SISSSPRKVN NEIHLTVAIV KYHSQDGEGD ERFGVCSNYL ASLNAKDDVY
LFVRSAPGFH LPSDTTRPII LIGPGTGIAP FRSFWQEFEM MREMNGECEL PKVWLFFGCR
SHSVDLYAEE KKRLVEEKIL DRVFLALSRE PDMKKTYVQD LIELEFDSLY NLIVEQRGHI
YVCGDVTMAE HVYQTIRKCI AGKEQKTEAE VETFLLTLRD ENRYHEDIFG ITLRTAEIHT
KSRATARIRM ASQP
//
