ID A0A1L0GD51_9ASCO Unreviewed; 258 AA.
AC A0A1L0GD51;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=NEDD8-conjugating enzyme UBC12 {ECO:0000256|ARBA:ARBA00044092};
DE EC=2.3.2.34 {ECO:0000256|ARBA:ARBA00044047};
DE AltName: Full=NEDD8-conjugating enzyme Ubc12 {ECO:0000256|ARBA:ARBA00044084};
DE AltName: Full=RUB1-conjugating enzyme {ECO:0000256|ARBA:ARBA00044279};
DE AltName: Full=Ubiquitin carrier protein 12 {ECO:0000256|ARBA:ARBA00044315};
GN ORFNames=SAMEA4029010_CIC11G00000001848 {ECO:0000313|EMBL:SGZ54253.1};
OS [Candida] intermedia.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Metschnikowiaceae; Metschnikowiaceae incertae sedis;
OC Candida/Metschnikowiaceae.
OX NCBI_TaxID=45354 {ECO:0000313|EMBL:SGZ54253.1, ECO:0000313|Proteomes:UP000182334};
RN [1] {ECO:0000313|EMBL:SGZ54253.1, ECO:0000313|Proteomes:UP000182334}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBS 141442 {ECO:0000313|EMBL:SGZ54253.1,
RC ECO:0000313|Proteomes:UP000182334};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine +
CC [E2 NEDD8-conjugating enzyme]-L-cysteine = [E1 NEDD8-activating
CC enzyme]-L-cysteine + [E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-
CC yl-L-cysteine.; EC=2.3.2.34;
CC Evidence={ECO:0000256|ARBA:ARBA00043698};
CC -!- PATHWAY: Protein modification; protein neddylation.
CC {ECO:0000256|ARBA:ARBA00005032}.
CC -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
CC {ECO:0000256|RuleBase:RU362109}.
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DR EMBL; LT635759; SGZ54253.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1L0GD51; -.
DR STRING; 45354.A0A1L0GD51; -.
DR OrthoDB; 46085at2759; -.
DR Proteomes; UP000182334; Chromosome iv.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR CDD; cd00195; UBCc; 1.
DR Gene3D; 3.10.110.10; Ubiquitin Conjugating Enzyme; 1.
DR InterPro; IPR000608; UBQ-conjugat_E2.
DR InterPro; IPR023313; UBQ-conjugating_AS.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR PANTHER; PTHR24067:SF261; NEDD8-CONJUGATING ENZYME UBC-12; 1.
DR PANTHER; PTHR24067; UBIQUITIN-CONJUGATING ENZYME E2; 1.
DR Pfam; PF00179; UQ_con; 1.
DR SMART; SM00212; UBCc; 1.
DR SUPFAM; SSF54495; UBC-like; 1.
DR PROSITE; PS00183; UBC_1; 1.
DR PROSITE; PS50127; UBC_2; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU362109};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU362109};
KW Reference proteome {ECO:0000313|Proteomes:UP000182334};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|RuleBase:RU362109}.
FT DOMAIN 105..249
FT /note="UBC core"
FT /evidence="ECO:0000259|PROSITE:PS50127"
FT ACT_SITE 187
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10133"
SQ SEQUENCE 258 AA; 29687 MW; ECE63BEB04C451A1 CRC64;
MANVHSMFSR TKQKNGFNQW LIHVEFICSH QKCRENALAF SLLEIAPNRY HRHTRTPHHK
ILQIVDCPTI TPSVLMQLKI KQLQKQKQEQ KLAGATSTTH TKVSAAHIRL QKDITELELP
QTIKIDFPNP ANLFQFNIAI RPSEGYYKSG TFRFTVDIGE NFPIDPPRIK CTQKIYHPNI
DLEGNVCLNI LREDWSPVLS LNSVLLGLNF LFLEPNPNDP LNKDAANMLV KNNPVFTRNV
RLAMKGLYFD GEQYDRVV
//
