ID A0A1R3UWG8_9ACTN Unreviewed; 483 AA.
AC A0A1R3UWG8;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE SubName: Full=Nitrite reductase [NAD(P)H] large subunit {ECO:0000313|EMBL:SIO89661.1};
DE EC=1.7.1.4 {ECO:0000313|EMBL:SIO89661.1};
GN ORFNames=BQ8420_22730 {ECO:0000313|EMBL:SIO89661.1};
OS Nocardiopsis sp. JB363.
OC Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
OC Nocardiopsaceae; Nocardiopsis.
OX NCBI_TaxID=1434837 {ECO:0000313|EMBL:SIO89661.1, ECO:0000313|Proteomes:UP000195722};
RN [1] {ECO:0000313|EMBL:SIO89661.1, ECO:0000313|Proteomes:UP000195722}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JB363 {ECO:0000313|EMBL:SIO89661.1,
RC ECO:0000313|Proteomes:UP000195722};
RA Song W.-J., Kurnit D.M.;
RL Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- COFACTOR:
CC Name=siroheme; Xref=ChEBI:CHEBI:60052;
CC Evidence={ECO:0000256|ARBA:ARBA00001929};
CC -!- PATHWAY: Nitrogen metabolism; nitrate reduction (assimilation).
CC {ECO:0000256|ARBA:ARBA00005096}.
CC -!- SIMILARITY: Belongs to the nitrite and sulfite reductase 4Fe-4S domain
CC family. {ECO:0000256|ARBA:ARBA00010429}.
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DR EMBL; FRFF01000138; SIO89661.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1R3UWG8; -.
DR OrthoDB; 9768666at2; -.
DR Proteomes; UP000195722; Unassembled WGS sequence.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0008942; F:nitrite reductase [NAD(P)H] activity; IEA:UniProtKB-EC.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR Gene3D; 3.30.390.30; -; 1.
DR Gene3D; 1.10.10.1100; BFD-like [2Fe-2S]-binding domain; 1.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 2.
DR InterPro; IPR007419; BFD-like_2Fe2S-bd_dom.
DR InterPro; IPR041854; BFD-like_2Fe2S-bd_dom_sf.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR023753; FAD/NAD-binding_dom.
DR InterPro; IPR016156; FAD/NAD-linked_Rdtase_dimer_sf.
DR InterPro; IPR041575; Rubredoxin_C.
DR PANTHER; PTHR43809; NITRITE REDUCTASE (NADH) LARGE SUBUNIT; 1.
DR PANTHER; PTHR43809:SF1; NITRITE REDUCTASE (NADH) LARGE SUBUNIT; 1.
DR Pfam; PF04324; Fer2_BFD; 1.
DR Pfam; PF07992; Pyr_redox_2; 1.
DR Pfam; PF18267; Rubredoxin_C; 1.
DR PRINTS; PR00368; FADPNR.
DR PRINTS; PR00411; PNDRDTASEI.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 2.
PE 3: Inferred from homology;
KW Heme {ECO:0000256|ARBA:ARBA00022617}; Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000313|EMBL:SIO89661.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000195722}.
FT DOMAIN 9..287
FT /note="FAD/NAD(P)-binding"
FT /evidence="ECO:0000259|Pfam:PF07992"
FT DOMAIN 323..380
FT /note="NADH-rubredoxin oxidoreductase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF18267"
FT DOMAIN 422..470
FT /note="BFD-like [2Fe-2S]-binding"
FT /evidence="ECO:0000259|Pfam:PF04324"
SQ SEQUENCE 483 AA; 50950 MW; 2CD6F7D4D7826582 CRC64;
MSTNPPPRRI VVIGNGMVGA RLAEEVAHLD PHGARVDLRV VGTEHHPAYN RVLLSQVLAG
THTPRQIRLP VPDTPGVSVR TGVTATSIDT THRRLRLDDG THLTFDELVL ATGARAALPP
ITGVSGHQGA PAEGVCALRD LDDCQRVLSL VRPGAPVVVL GGGVLGLEAA RGLVGRGARV
SLVESSPWIM RRQIDRPAAH ILARRYEALG VNVHSWRVAS RWIPGTGLEL DDGRVLAGDA
LVVTAGVRAN TELAKDAAIE VEHGVVVDDH LTTSHSRVHA IGDCAQHPGG GAGLVGPGWE
QAAVLARRLT GVEPGARYRG ARPLTRLKAQ GIELTAFGRV QEEDDGPEVE TVTVSDPHGG
RYAKLSVERD RVVGAVLLGF PEAAATLAQL YDTAMPVPAD RLALVQGHPA HTPHEQDPAP
ALVCRCNAVG REEIEQAWLD GARTREAIAH RTRATTGCGG CVRDVDALLE GMGGANTTPT
TIP
//
