ID   A4YZV2_BRASO            Unreviewed;       544 AA.
AC   A4YZV2;
DT   29-MAY-2007, integrated into UniProtKB/TrEMBL.
DT   29-MAY-2007, sequence version 1.
DT   27-MAR-2024, entry version 93.
DE   SubName: Full=Putative choline (Or alcohol) dehydrogenase {ECO:0000313|EMBL:CAL79428.1};
DE            EC=1.1.99.1 {ECO:0000313|EMBL:CAL79428.1};
GN   OrderedLocusNames=BRADO5760 {ECO:0000313|EMBL:CAL79428.1};
OS   Bradyrhizobium sp. (strain ORS 278).
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC   Nitrobacteraceae; Bradyrhizobium.
OX   NCBI_TaxID=114615 {ECO:0000313|EMBL:CAL79428.1, ECO:0000313|Proteomes:UP000001994};
RN   [1] {ECO:0000313|EMBL:CAL79428.1, ECO:0000313|Proteomes:UP000001994}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ORS 278 {ECO:0000313|Proteomes:UP000001994};
RX   PubMed=17540897; DOI=10.1126/science.1139548;
RA   Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.C.,
RA   Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L.,
RA   Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z.,
RA   Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.S., Saunders E.,
RA   Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G.,
RA   Emerich D., Vermeglio A., Medigue C., Sadowsky M.;
RT   "Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia.";
RL   Science 316:1307-1312(2007).
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000256|ARBA:ARBA00001974,
CC         ECO:0000256|PIRSR:PIRSR000137-2};
CC   -!- SIMILARITY: Belongs to the GMC oxidoreductase family.
CC       {ECO:0000256|ARBA:ARBA00010790, ECO:0000256|RuleBase:RU003968}.
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DR   EMBL; CU234118; CAL79428.1; -; Genomic_DNA.
DR   RefSeq; WP_012029332.1; NC_009445.1.
DR   AlphaFoldDB; A4YZV2; -.
DR   STRING; 114615.BRADO5760; -.
DR   KEGG; bra:BRADO5760; -.
DR   eggNOG; COG2303; Bacteria.
DR   HOGENOM; CLU_002865_7_2_5; -.
DR   OrthoDB; 9785276at2; -.
DR   Proteomes; UP000001994; Chromosome.
DR   GO; GO:0008812; F:choline dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR   Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR   Gene3D; 3.30.560.10; Glucose Oxidase, domain 3; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR012132; GMC_OxRdtase.
DR   InterPro; IPR000172; GMC_OxRdtase_N.
DR   InterPro; IPR007867; GMC_OxRtase_C.
DR   PANTHER; PTHR11552:SF147; CHOLINE DEHYDROGENASE, MITOCHONDRIAL; 1.
DR   PANTHER; PTHR11552; GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE; 1.
DR   Pfam; PF05199; GMC_oxred_C; 1.
DR   Pfam; PF00732; GMC_oxred_N; 1.
DR   PIRSF; PIRSF000137; Alcohol_oxidase; 1.
DR   SUPFAM; SSF54373; FAD-linked reductases, C-terminal domain; 1.
DR   SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR   PROSITE; PS00623; GMC_OXRED_1; 1.
DR   PROSITE; PS00624; GMC_OXRED_2; 1.
PE   3: Inferred from homology;
KW   FAD {ECO:0000256|ARBA:ARBA00022827, ECO:0000256|PIRSR:PIRSR000137-2};
KW   Flavoprotein {ECO:0000256|ARBA:ARBA00022630,
KW   ECO:0000256|RuleBase:RU003968};
KW   Oxidoreductase {ECO:0000313|EMBL:CAL79428.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001994}.
FT   DOMAIN          81..104
FT                   /note="Glucose-methanol-choline oxidoreductase N-terminal"
FT                   /evidence="ECO:0000259|PROSITE:PS00623"
FT   DOMAIN          257..271
FT                   /note="Glucose-methanol-choline oxidoreductase N-terminal"
FT                   /evidence="ECO:0000259|PROSITE:PS00624"
FT   BINDING         91..94
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000137-2"
SQ   SEQUENCE   544 AA;  59692 MW;  45B8F50D43A4F8E8 CRC64;
     MDTFDYVIVG AGSAGCVIAS RLSEDPSVTV CVLEAGPRDW HPYIHLPAGF IKTFYMKSIN
     WGYQQEPGPY TAGRSIYAPR GKTLGGSSSI NGHVYNRGQS QDFDTWAQLG NRGWSYSDVL
     PHFRRMEKRI GAGDEQYRGR DGSLHVTTME WKDTLCEAFM DGAVSLGIPR NPDYNGAIQE
     GVSYVQRTIQ NGRRVSSATA FLKPASKRPN VEIRTHAHAT SIMLEGKRAV GVRYHRGGRG
     GVPVEVRARK EVILSGGTYN SPQLLQLSGI GAPELLQEHG IEVRHALKSV GEGLQDHYAP
     RTVARVKNIK TINELARGLN LWGEALKWAV TRKGILSLSP TMVYCFWHSG ETAESSDLQL
     TFTPASYKEG VQGQLEDEPG MTVASWQQRP ESRGYVRIRS ADPFAPPLIQ TNYLTAELDR
     RVIVAGMKLA RRLLASAPLA PYYAYEDFPG PKVNSDDEFL AAATQRATTT FHPGCTCRMG
     PAESTWATVD DQLRVHGLQG LRIADASIMP RMISANLNAA TLMIGDRAAD LILGKVQAGE
     RLSA
//