ID G1SRH2_RABIT Unreviewed; 348 AA.
AC G1SRH2;
DT 19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT 19-OCT-2011, sequence version 1.
DT 27-MAR-2024, entry version 63.
DE RecName: Full=Lactosylceramide 4-alpha-galactosyltransferase {ECO:0000256|ARBA:ARBA00040835};
DE EC=2.4.1.228 {ECO:0000256|ARBA:ARBA00039051};
DE AltName: Full=Alpha-1,4-N-acetylglucosaminyltransferase {ECO:0000256|ARBA:ARBA00043186};
DE AltName: Full=Alpha-1,4-galactosyltransferase {ECO:0000256|ARBA:ARBA00041849};
DE AltName: Full=Globotriaosylceramide synthase {ECO:0000256|ARBA:ARBA00043154};
DE AltName: Full=UDP-galactose:beta-D-galactosyl-beta1-R 4-alpha-D-galactosyltransferase {ECO:0000256|ARBA:ARBA00041556};
GN Name=A4GALT {ECO:0000313|Ensembl:ENSOCUP00000005770.2};
OS Oryctolagus cuniculus (Rabbit).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Lagomorpha; Leporidae; Oryctolagus.
OX NCBI_TaxID=9986 {ECO:0000313|Ensembl:ENSOCUP00000005770.2, ECO:0000313|Proteomes:UP000001811};
RN [1] {ECO:0000313|Ensembl:ENSOCUP00000005770.2, ECO:0000313|Proteomes:UP000001811}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Thorbecke {ECO:0000313|Proteomes:UP000001811};
RX PubMed=21993624; DOI=10.1038/nature10530;
RA Lindblad-Toh K., Garber M., Zuk O., Lin M.F., Parker B.J., Washietl S.,
RA Kheradpour P., Ernst J., Jordan G., Mauceli E., Ward L.D., Lowe C.B.,
RA Holloway A.K., Clamp M., Gnerre S., Alfoldi J., Beal K., Chang J.,
RA Clawson H., Cuff J., Di Palma F., Fitzgerald S., Flicek P., Guttman M.,
RA Hubisz M.J., Jaffe D.B., Jungreis I., Kent W.J., Kostka D., Lara M.,
RA Martins A.L., Massingham T., Moltke I., Raney B.J., Rasmussen M.D.,
RA Robinson J., Stark A., Vilella A.J., Wen J., Xie X., Zody M.C., Baldwin J.,
RA Bloom T., Chin C.W., Heiman D., Nicol R., Nusbaum C., Young S.,
RA Wilkinson J., Worley K.C., Kovar C.L., Muzny D.M., Gibbs R.A., Cree A.,
RA Dihn H.H., Fowler G., Jhangiani S., Joshi V., Lee S., Lewis L.R.,
RA Nazareth L.V., Okwuonu G., Santibanez J., Warren W.C., Mardis E.R.,
RA Weinstock G.M., Wilson R.K., Delehaunty K., Dooling D., Fronik C.,
RA Fulton L., Fulton B., Graves T., Minx P., Sodergren E., Birney E.,
RA Margulies E.H., Herrero J., Green E.D., Haussler D., Siepel A., Goldman N.,
RA Pollard K.S., Pedersen J.S., Lander E.S., Kellis M.;
RT "A high-resolution map of human evolutionary constraint using 29 mammals.";
RL Nature 478:476-482(2011).
RN [2] {ECO:0000313|Ensembl:ENSOCUP00000005770.2}
RP IDENTIFICATION.
RC STRAIN=Thorbecke {ECO:0000313|Ensembl:ENSOCUP00000005770.2};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-Cer(d18:1(4E)) + UDP-
CC alpha-D-galactose = a globoside Gb3Cer (d18:1(4E)) + H(+) + UDP;
CC Xref=Rhea:RHEA:11924, ChEBI:CHEBI:15378, ChEBI:CHEBI:17950,
CC ChEBI:CHEBI:18313, ChEBI:CHEBI:58223, ChEBI:CHEBI:66914;
CC EC=2.4.1.228; Evidence={ECO:0000256|ARBA:ARBA00043770};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:11925;
CC Evidence={ECO:0000256|ARBA:ARBA00043770};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=beta-D-Gal-(1<->1')-Cer + UDP-alpha-D-galactose = alpha-D-Gal-
CC (1->4)-beta-D-Gal-(1<->1')-Cer + H(+) + UDP; Xref=Rhea:RHEA:60044,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58223, ChEBI:CHEBI:66914,
CC ChEBI:CHEBI:143593, ChEBI:CHEBI:143594;
CC Evidence={ECO:0000256|ARBA:ARBA00036489};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60045;
CC Evidence={ECO:0000256|ARBA:ARBA00036489};
CC -!- PATHWAY: Glycolipid biosynthesis. {ECO:0000256|ARBA:ARBA00004934}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000256|ARBA:ARBA00004323}; Single-pass type II membrane protein
CC {ECO:0000256|ARBA:ARBA00004323}. Membrane
CC {ECO:0000256|ARBA:ARBA00004606}; Single-pass type II membrane protein
CC {ECO:0000256|ARBA:ARBA00004606}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR RefSeq; XP_008273357.1; XM_008275135.2.
DR AlphaFoldDB; G1SRH2; -.
DR STRING; 9986.ENSOCUP00000005770; -.
DR PaxDb; 9986-ENSOCUP00000005770; -.
DR Ensembl; ENSOCUT00000006674.2; ENSOCUP00000005770.2; ENSOCUG00000006673.2.
DR GeneID; 100347783; -.
DR KEGG; ocu:100347783; -.
DR CTD; 53947; -.
DR eggNOG; KOG1928; Eukaryota.
DR GeneTree; ENSGT00510000047981; -.
DR HOGENOM; CLU_049512_2_0_1; -.
DR InParanoid; G1SRH2; -.
DR OMA; CKDSYVV; -.
DR OrthoDB; 2235518at2759; -.
DR TreeFam; TF324053; -.
DR Proteomes; UP000001811; Unplaced.
DR Bgee; ENSOCUG00000006673; Expressed in aorta and 14 other cell types or tissues.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008378; F:galactosyltransferase activity; IEA:Ensembl.
DR GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0007009; P:plasma membrane organization; IEA:Ensembl.
DR Gene3D; 3.90.550.20; -; 1.
DR InterPro; IPR007652; A1-4-GlycosylTfrase_dom.
DR InterPro; IPR007577; GlycoTrfase_DXD_sugar-bd_CS.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR PANTHER; PTHR12042:SF17; LACTOSYLCERAMIDE 4-ALPHA-GALACTOSYLTRANSFERASE; 1.
DR PANTHER; PTHR12042; LACTOSYLCERAMIDE 4-ALPHA-GALACTOSYLTRANSFERASE ALPHA- 1,4-GALACTOSYLTRANSFERASE; 1.
DR Pfam; PF04572; Gb3_synth; 1.
DR Pfam; PF04488; Gly_transf_sug; 1.
DR SUPFAM; SSF53448; Nucleotide-diphospho-sugar transferases; 1.
PE 4: Predicted;
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Golgi apparatus {ECO:0000256|ARBA:ARBA00023034};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000001811};
KW Transferase {ECO:0000256|ARBA:ARBA00022676};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 20..42
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 217..344
FT /note="Alpha 1,4-glycosyltransferase"
FT /evidence="ECO:0000259|Pfam:PF04572"
SQ SEQUENCE 348 AA; 39761 MW; D0725246DF2B49E3 CRC64;
MSRPPDWLLR LLPSAPRQRV CTLFVISFKF TFLISVVIYW HVAGAPGDPG RLQSLPAQVP
CPTLEPRPPT LGSIFFLETS ERTNPNFLFM CSVESAARTH PEARVVVLMK GLPAGNASLP
RHLGISLLGC FPNVQMLPLD LEELFRGTPL AAWYAAAQRR WEPYLLPVLS DASRIALLWK
FGGIYLDTDF IVLRSLRNLT NALGTQSRYV LNGAFLAFER RHEFLALCMR DFVAHYNRWV
WGHQGPQLLT RVFKKWCATR RLADSHACRG VTALPREAFY PIAWQDWKKY FEDVSPEELA
RMLNATYAVH VWNKKSQGTR FEATSRALLA QLHARYCPTT HKAMKMYL
//
