ID HRPB_ECOLI Reviewed; 809 AA.
AC P37024; P75663; Q2MCG2;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 3.
DT 27-MAR-2024, entry version 184.
DE RecName: Full=ATP-dependent RNA helicase HrpB;
DE EC=3.6.4.13;
GN Name=hrpB; Synonyms=yadO; OrderedLocusNames=b0148, JW0144;
OS Escherichia coli (strain K12).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8202364; DOI=10.1093/nar/22.9.1637;
RA Fujita N., Mori H., Yura T., Ishihama A.;
RT "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-
RT 4.1 min (110,917-193,643 bp) region.";
RL Nucleic Acids Res. 22:1637-1639(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 61-156.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=7899078; DOI=10.1093/nar/23.4.595;
RA Moriya H., Kasai H., Isono K.;
RT "Cloning and characterization of the hrpA gene in the terC region of
RT Escherichia coli that is highly similar to the DEAH family RNA helicase
RT genes of Saccharomyces cerevisiae.";
RL Nucleic Acids Res. 23:595-598(1995).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. {ECO:0000305}.
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DR EMBL; U00096; AAC73259.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE76044.1; -; Genomic_DNA.
DR EMBL; D42122; BAA21040.1; -; Genomic_DNA.
DR PIR; D64738; D64738.
DR RefSeq; NP_414690.4; NC_000913.3.
DR RefSeq; WP_001350480.1; NZ_LN832404.1.
DR PDB; 6EUD; X-ray; 2.40 A; A=1-809.
DR PDB; 6HEG; X-ray; 3.02 A; A=1-809.
DR PDBsum; 6EUD; -.
DR PDBsum; 6HEG; -.
DR AlphaFoldDB; P37024; -.
DR SMR; P37024; -.
DR BioGRID; 4261401; 85.
DR DIP; DIP-9938N; -.
DR IntAct; P37024; 10.
DR STRING; 511145.b0148; -.
DR PaxDb; 511145-b0148; -.
DR EnsemblBacteria; AAC73259; AAC73259; b0148.
DR GeneID; 944845; -.
DR KEGG; ecj:JW0144; -.
DR KEGG; eco:b0148; -.
DR PATRIC; fig|1411691.4.peg.2133; -.
DR EchoBASE; EB2233; -.
DR eggNOG; COG1643; Bacteria.
DR HOGENOM; CLU_001832_5_6_6; -.
DR InParanoid; P37024; -.
DR OMA; VCYRLWD; -.
DR OrthoDB; 9805617at2; -.
DR PhylomeDB; P37024; -.
DR BioCyc; EcoCyc:EG12329-MONOMER; -.
DR BRENDA; 3.6.1.15; 2026.
DR PRO; PR:P37024; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0017111; F:ribonucleoside triphosphate phosphatase activity; IDA:EcoCyc.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0003727; F:single-stranded RNA binding; IDA:EcoCyc.
DR CDD; cd17990; DEXHc_HrpB; 1.
DR CDD; cd18791; SF2_C_RHA; 1.
DR Gene3D; 1.20.120.1080; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR007502; Helicase-assoc_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR010225; HrpB.
DR InterPro; IPR049614; HrpB_DEXH.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR013689; RNA_helicase_ATP-dep_HrpB_C.
DR NCBIfam; TIGR01970; DEAH_box_HrpB; 1.
DR PANTHER; PTHR43519; ATP-DEPENDENT RNA HELICASE HRPB; 1.
DR PANTHER; PTHR43519:SF1; ATP-DEPENDENT RNA HELICASE HRPB; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF08482; HrpB_C; 1.
DR PIRSF; PIRSF005496; ATP_hel_hrpB; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00847; HA2; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Helicase; Hydrolase; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..809
FT /note="ATP-dependent RNA helicase HrpB"
FT /id="PRO_0000055180"
FT DOMAIN 14..177
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 195..368
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 788..809
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 123..126
FT /note="DEFH box"
FT BINDING 27..34
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT HELIX 7..9
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 10..17
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 23..26
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 29..31
FT /evidence="ECO:0007829|PDB:6EUD"
FT TURN 33..36
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 37..42
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 49..54
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 58..71
FT /evidence="ECO:0007829|PDB:6EUD"
FT TURN 75..77
FT /evidence="ECO:0007829|PDB:6HEG"
FT STRAND 78..84
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 87..89
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 95..99
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 103..110
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 117..122
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 125..127
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 130..142
FT /evidence="ECO:0007829|PDB:6EUD"
FT TURN 143..146
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 151..159
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 164..167
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 173..175
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 183..187
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 192..194
FT /evidence="ECO:0007829|PDB:6HEG"
FT HELIX 196..210
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 213..218
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 222..233
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 240..245
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 253..258
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 266..271
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 273..275
FT /evidence="ECO:0007829|PDB:6EUD"
FT TURN 276..278
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 283..289
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 292..298
FT /evidence="ECO:0007829|PDB:6EUD"
FT TURN 300..302
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 305..311
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 314..322
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 325..336
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 338..343
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 351..353
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 358..367
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 372..374
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 383..395
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 406..414
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 418..426
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 430..444
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 448..450
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 454..459
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 463..476
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 485..487
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 488..495
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 497..499
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 500..503
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 509..512
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 517..519
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 525..528
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 529..539
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 544..553
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 556..562
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 564..566
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 568..576
FT /evidence="ECO:0007829|PDB:6EUD"
FT TURN 577..580
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 581..590
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 593..599
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 605..619
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 621..623
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 628..643
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 654..659
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 661..664
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 666..668
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 675..680
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 683..689
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 693..702
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 705..708
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 714..718
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 721..723
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 726..730
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 731..734
FT /evidence="ECO:0007829|PDB:6EUD"
FT TURN 744..747
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 751..755
FT /evidence="ECO:0007829|PDB:6EUD"
FT STRAND 761..766
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 768..773
FT /evidence="ECO:0007829|PDB:6EUD"
FT HELIX 776..786
FT /evidence="ECO:0007829|PDB:6EUD"
FT TURN 788..790
FT /evidence="ECO:0007829|PDB:6HEG"
FT TURN 796..798
FT /evidence="ECO:0007829|PDB:6EUD"
SQ SEQUENCE 809 AA; 89148 MW; 413605E1D2D90715 CRC64;
MSSLPVAAVL PELLTALDCA PQVLLSAPTG AGKSTWLPLQ LLAHPGINGK IILLEPRRLA
ARNVAQRLAE LLNEKPGDTV GYRMRAQNCV GPNTRLEVVT EGVLTRMIQR DPELSGVGLV
ILDEFHERSL QADLALALLL DVQQGLRDDL KLLIMSATLD NDRLQQMLPE APVVISEGRS
FPVERRYLPL PAHQRFDDAV AVATAEMLRQ ESGSLLLFLP GVGEIQRVQE QLASRIGSDV
LLCPLYGALS LNDQRKAILP APQGMRKVVL ATNIAETSLT IEGIRLVVDC AQERVARFDP
RTGLTRLITQ RVSQASMTQR AGRAGRLEPG ISLHLIAKEQ AERAAAQSEP EILQSDLSGL
LMELLQWGCS DPAQMSWLDQ PPVVNLLAAK RLLQMLGALE GERLSAQGQK MAALGNDPRL
AAMLVSAKND DEAATAAKIA AILEEPPRMG NSDLGVAFSR NQPAWQQRSQ QLLKRLNVRG
GEADSSLIAP LLAGAFADRI ARRRGQDGRY QLANGMGAML DANDALSRHE WLIAPLLLQG
SASPDARILL ALLVDIDELV QRCPQLVQQS DTVEWDDAQG TLKAWRRLQI GQLTVKVQPL
AKPSEDELHQ AMLNGIRDKG LSVLNWTAEA EQLRLRLLCA AKWLPEYDWP AVDDESLLAA
LETWLLPHMT GVHSLRGLKS LDIYQALRGL LDWGMQQRLD SELPAHYTVP TGSRIAIRYH
EDNPPALAVR MQEMFGEATN PTIAQGRVPL VLELLSPAQR PLQITRDLSD FWKGAYREVQ
KEMKGRYPKH VWPDDPANTA PTRRTKKYS
//
