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Database: UniProt
Entry: Q53RK8
LinkDB: Q53RK8
Original site: Q53RK8 
ID   RH21_ORYSJ              Reviewed;         736 AA.
AC   Q53RK8; B7EG14;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   31-JUL-2019, entry version 102.
DE   RecName: Full=DEAD-box ATP-dependent RNA helicase 21;
DE            EC=3.6.4.13;
GN   OrderedLocusNames=Os03g0708600, LOC_Os03g50090; ORFNames=OsJ_12293;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16109971; DOI=10.1101/gr.3869505;
RG   The rice chromosome 3 sequencing consortium;
RA   Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R.,
RA   Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L.,
RA   Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S., Johri S.,
RA   Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S.,
RA   Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J.,
RA   de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H.,
RA   Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F.,
RA   Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A.,
RA   Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J.,
RA   Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L.,
RA   Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A.,
RA   Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W.,
RA   Lee H.R., Jiang J., Jackson S.;
RT   "Sequence, annotation, and analysis of synteny between rice chromosome
RT   3 and diverged grass species.";
RL   Genome Res. 15:1284-1291(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H.,
RA   McCombie W.R., Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S.,
RA   Childs K.L., Davidson R.M., Lin H., Quesada-Ocampo L.,
RA   Vaillancourt B., Sakai H., Lee S.S., Kim J., Numa H., Itoh T.,
RA   Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using
RT   next generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H.,
RA   Cong L., Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J.,
RA   Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X.,
RA   Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y.,
RA   Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J.,
RA   Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y.,
RA   Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y.,
RA   Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z.,
RA   Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T.,
RA   Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H.,
RA   Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W.,
RA   Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L.,
RA   Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J.,
RA   Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- FUNCTION: ATP-dependent RNA helicase involved in mRNA splicing.
CC       May destabilize the U1/5'-splice site duplex to permit an
CC       effective competition for the 5'-splice site by the U6 snRNA,
CC       resulting in the switch between U1 and U6 at the 5'-splice site.
CC       May also act to unwind the U4/U6 base-pairing interaction in the
CC       U4/U6/U5 snRNP, facilitating the first covalent step of splicing
CC       (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus
CC       {ECO:0000250}.
CC   -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD
CC       box family of RNA helicases and controls ATP binding and
CC       hydrolysis.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX23/PRP28
CC       subfamily. {ECO:0000305}.
DR   EMBL; AC087181; AAX95674.1; -; Genomic_DNA.
DR   EMBL; DP000009; ABF98484.1; -; Genomic_DNA.
DR   EMBL; DP000009; ABF98485.1; -; Genomic_DNA.
DR   EMBL; DP000009; ABF98486.1; -; Genomic_DNA.
DR   EMBL; AP008209; BAF12952.1; -; Genomic_DNA.
DR   EMBL; AP014959; BAS85997.1; -; Genomic_DNA.
DR   EMBL; CM000140; EAZ28319.1; -; Genomic_DNA.
DR   EMBL; AK069199; BAG91311.1; -; mRNA.
DR   RefSeq; XP_015631547.1; XM_015776061.1.
DR   RefSeq; XP_015631548.1; XM_015776062.1.
DR   RefSeq; XP_015631549.1; XM_015776063.1.
DR   SMR; Q53RK8; -.
DR   STRING; 4530.OS03T0708600-02; -.
DR   PaxDb; Q53RK8; -.
DR   PRIDE; Q53RK8; -.
DR   EnsemblPlants; Os03t0708600-01; Os03t0708600-01; Os03g0708600.
DR   EnsemblPlants; Os03t0708600-02; Os03t0708600-02; Os03g0708600.
DR   GeneID; 4333866; -.
DR   Gramene; Os03t0708600-01; Os03t0708600-01; Os03g0708600.
DR   Gramene; Os03t0708600-02; Os03t0708600-02; Os03g0708600.
DR   KEGG; osa:4333866; -.
DR   eggNOG; KOG0333; Eukaryota.
DR   eggNOG; ENOG410XNVG; LUCA.
DR   HOGENOM; HOG000268796; -.
DR   InParanoid; Q53RK8; -.
DR   KO; K12858; -.
DR   OMA; TEKKFNF; -.
DR   OrthoDB; 820037at2759; -.
DR   Proteomes; UP000059680; Chromosome 3.
DR   Genevisible; Q53RK8; OS.
DR   GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Coiled coil; Complete proteome; Cytoplasm; Helicase;
KW   Hydrolase; mRNA processing; mRNA splicing; Nucleotide-binding;
KW   Nucleus; Reference proteome; RNA-binding.
FT   CHAIN         1    736       DEAD-box ATP-dependent RNA helicase 21.
FT                                /FTId=PRO_0000282477.
FT   DOMAIN      346    541       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      568    712       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND     359    366       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   COILED       14     38       {ECO:0000255}.
FT   COILED      137    167       {ECO:0000255}.
FT   MOTIF       315    343       Q motif.
FT   MOTIF       472    475       DEAD box.
FT   COMPBIAS     46     60       Pro-rich.
SQ   SEQUENCE   736 AA;  84521 MW;  5D7ED635FC1AD629 CRC64;
     MKRAADGMEK PKFLTREERE KLALERRQAA VTDQRRSALD LLQSLPRPPP PPPPPLSNPP
     RDSSSSHHRD SSDRDRDRDR DRDRDRDRDR DRERRRDDDS RRDRDRDRDR DRGDSSRRDR
     DRERGDRDRD RDRERGDRDR ERGDREKDRL EKMAEREREK ELDAIKEQYL GSKKPKKRVI
     KPSEKFRFSF DWENTEDTSR DMNSLYQSPH EARLLYGRGF LAGIDRREQK KVAAAHEKET
     RAEQRRKAGL DDRPEDDAVD KKEADAAAKY DAFDMRVDRH WTQKSLDEMT ERDWRIFRED
     FNISYKGSKV PRPMRKWSES KLGTELLRAV EKAGYKEPSP IQMASIPLGL QQRDVIGIAE
     TGSGKTAAFV LPMLSYITRL PPISEENEAE GPYAVVMAPT RELAQQIEEE TVKFATYLGI
     KVVSIVGGQS IEEQGFKIRQ GCEVVIATPG RLLDCLERRY AVLNQCNYVV LDEADRMIDM
     GFEPQVVGVL DAMPSSNLKP ENEDEELDAK TIYRTTYMFS ATMPPAVERL ARKYLRNPVV
     VTIGTAGKAT DLITQNVIMT KESEKMSRLQ KILTDLGDKP AIVFCNTKKS ADARAKDLDK
     AGFRVTTLHG GKSQEQRETS LDGFRNRRFT VLVATDVAGR GIDIPDVAHV INYEMPSSID
     TYTHRIGRTG RAGKKGLATS FLTLENTDIF FDLKQMLIQS NSPVPPELAR HEASKFKPGS
     VPDRPPRRND TVYATH
//
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