ID CDC20_RAT Reviewed; 499 AA.
AC Q62623; O70380; Q5U360;
DT 03-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2007, sequence version 2.
DT 24-JAN-2024, entry version 168.
DE RecName: Full=Cell division cycle protein 20 homolog;
DE AltName: Full=p55CDC;
GN Name=Cdc20;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Spleen;
RX PubMed=7513050; DOI=10.1128/mcb.14.5.3350-3363.1994;
RA Weinstein J., Jacobsen F.W., Hsu-Chen J., Wu T., Baum L.G.;
RT "A novel mammalian protein, p55CDC, present in dividing cells is associated
RT with protein kinase activity and has homology to the Saccharomyces
RT cerevisiae cell division cycle proteins Cdc20 and Cdc4.";
RL Mol. Cell. Biol. 14:3350-3363(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9682218; DOI=10.1006/mgme.1998.2698;
RA Weinstein J., Karim J., Geschwind D.H., Nelson S.F., Krumm J.,
RA Sakamoto K.M.;
RT "Genomic organization, 5' flanking enhancer region, and chromosomal
RT assignment of the cell cycle gene, p55Cdc.";
RL Mol. Genet. Metab. 64:52-57(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Involved in the metaphase/anaphase transition of cell cycle
CC (By similarity). Required for full ubiquitin ligase activity of the
CC anaphase promoting complex/cyclosome (APC/C) and may confer substrate
CC specificity upon the complex. Is regulated by MAD2L1: in metaphase the
CC MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the
CC CDC20-APC/C binary complex is active in degrading substrates. The
CC CDC20-APC/C complex positively regulates the formation of synaptic
CC vesicle clustering at active zone to the presynaptic membrane in
CC postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces
CC presynaptic differentiation (By similarity). The CDC20-APC/C complex
CC promotes proper dilation formation and radial migration by degrading
CC CCDC41 (By similarity). {ECO:0000250|UniProtKB:Q12834,
CC ECO:0000250|UniProtKB:Q9JJ66}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Component of a complex with CDC20, CDC27, SPATC1 and TUBG1 (By
CC similarity). Interacts with NEUROD2 (By similarity). Interacts with
CC dimeric MAD2L1 in its closed conformation form (By similarity).
CC Interacts with BUB1B (By similarity). The phosphorylated form interacts
CC with APC/C (By similarity). Interacts with NINL (By similarity). May
CC interact with MAD2L2 (By similarity). Interacts with CDK5RAP2 (By
CC similarity). Interacts with SIRT2 (By similarity). Interacts with
CC isoform 1 of NEK2 (By similarity). Interacts with HSF1 (via
CC phosphorylated form); this interaction occurs in mitosis in a MAD2L1-
CC dependent manner and prevents PLK1-stimulated degradation of HSF1 by
CC blocking the recruitment of the SCF(BTRC) ubiquitin ligase complex (By
CC similarity). Interacts (via the N-terminal substrate-binding domain)
CC with FBXO5 (By similarity). Interacts with CCNF (By similarity).
CC {ECO:0000250|UniProtKB:Q12834, ECO:0000250|UniProtKB:Q9JJ66}.
CC -!- INTERACTION:
CC Q62623; Q9UBN7: HDAC6; Xeno; NbExp=2; IntAct=EBI-2256532, EBI-301697;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome {ECO:0000250|UniProtKB:Q12834}. Chromosome,
CC centromere, kinetochore {ECO:0000250|UniProtKB:Q12834}. Cytoplasm,
CC cytoskeleton, spindle pole {ECO:0000250|UniProtKB:Q12834}.
CC -!- PTM: Phosphorylated during mitosis (By similarity). Phosphorylated by
CC BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161 (By
CC similarity). Phosphorylated by NEK2 (By similarity).
CC {ECO:0000250|UniProtKB:Q12834}.
CC -!- PTM: Dephosphorylated by CTDP1. {ECO:0000250}.
CC -!- PTM: Acetylated. Deacetylated at Lys-66 by SIRT2; deacetylation
CC enhances the interaction of CDC20 with CDC27, leading to activation of
CC anaphase promoting complex/cyclosome (APC/C) (By similarity).
CC {ECO:0000250}.
CC -!- PTM: Ubiquitinated and degraded by the proteasome during spindle
CC assembly checkpoint. Ubiquitinated at Lys-490 during prometaphase.
CC Ubiquitination at Lys-485 and Lys-490 has no effect on its ability to
CC bind the APC/C complex (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the WD repeat CDC20/Fizzy family. {ECO:0000305}.
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DR EMBL; U05341; AAA19018.1; -; mRNA.
DR EMBL; AF052695; AAC14741.1; -; Genomic_DNA.
DR EMBL; BC085691; AAH85691.1; -; mRNA.
DR PIR; B56021; B56021.
DR RefSeq; NP_741990.1; NM_171993.1.
DR AlphaFoldDB; Q62623; -.
DR SMR; Q62623; -.
DR BioGRID; 249100; 6.
DR IntAct; Q62623; 3.
DR STRING; 10116.ENSRNOP00000037772; -.
DR iPTMnet; Q62623; -.
DR PhosphoSitePlus; Q62623; -.
DR PaxDb; 10116-ENSRNOP00000037772; -.
DR Ensembl; ENSRNOT00055022186; ENSRNOP00055018026; ENSRNOG00055012949.
DR Ensembl; ENSRNOT00060024164; ENSRNOP00060019279; ENSRNOG00060014125.
DR Ensembl; ENSRNOT00065029026; ENSRNOP00065022990; ENSRNOG00065017386.
DR GeneID; 64515; -.
DR KEGG; rno:64515; -.
DR UCSC; RGD:620477; rat.
DR AGR; RGD:620477; -.
DR CTD; 991; -.
DR RGD; 620477; Cdc20.
DR VEuPathDB; HostDB:ENSRNOG00000028415; -.
DR eggNOG; KOG0305; Eukaryota.
DR HOGENOM; CLU_014831_6_1_1; -.
DR InParanoid; Q62623; -.
DR OrthoDB; 20041at2759; -.
DR PhylomeDB; Q62623; -.
DR Reactome; R-RNO-141405; Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components.
DR Reactome; R-RNO-141430; Inactivation of APC/C via direct inhibition of the APC/C complex.
DR Reactome; R-RNO-141444; Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
DR Reactome; R-RNO-174048; APC/C:Cdc20 mediated degradation of Cyclin B.
DR Reactome; R-RNO-174113; SCF-beta-TrCP mediated degradation of Emi1.
DR Reactome; R-RNO-174154; APC/C:Cdc20 mediated degradation of Securin.
DR Reactome; R-RNO-174178; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
DR Reactome; R-RNO-174184; Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
DR Reactome; R-RNO-176407; Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
DR Reactome; R-RNO-176408; Regulation of APC/C activators between G1/S and early anaphase.
DR Reactome; R-RNO-176417; Phosphorylation of Emi1.
DR Reactome; R-RNO-179409; APC-Cdc20 mediated degradation of Nek2A.
DR Reactome; R-RNO-2467813; Separation of Sister Chromatids.
DR Reactome; R-RNO-2500257; Resolution of Sister Chromatid Cohesion.
DR Reactome; R-RNO-5663220; RHO GTPases Activate Formins.
DR Reactome; R-RNO-5689880; Ub-specific processing proteases.
DR Reactome; R-RNO-68877; Mitotic Prometaphase.
DR Reactome; R-RNO-9648025; EML4 and NUDC in mitotic spindle formation.
DR Reactome; R-RNO-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q62623; -.
DR Proteomes; UP000002494; Chromosome 5.
DR Bgee; ENSRNOG00000028415; Expressed in testis and 19 other cell types or tissues.
DR Genevisible; Q62623; RN.
DR GO; GO:0005680; C:anaphase-promoting complex; ISO:RGD.
DR GO; GO:0005813; C:centrosome; IDA:RGD.
DR GO; GO:0000776; C:kinetochore; ISS:UniProtKB.
DR GO; GO:0033597; C:mitotic checkpoint complex; ISO:RGD.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:RGD.
DR GO; GO:0032991; C:protein-containing complex; IDA:RGD.
DR GO; GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
DR GO; GO:0010997; F:anaphase-promoting complex binding; ISO:RGD.
DR GO; GO:0042826; F:histone deacetylase binding; IPI:RGD.
DR GO; GO:1990757; F:ubiquitin ligase activator activity; IBA:GO_Central.
DR GO; GO:0031145; P:anaphase-promoting complex-dependent catabolic process; ISO:RGD.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0044784; P:metaphase/anaphase transition of cell cycle; ISS:UniProtKB.
DR GO; GO:1990949; P:metaphase/anaphase transition of meiosis I; ISS:UniProtKB.
DR GO; GO:0007064; P:mitotic sister chromatid cohesion; ISO:RGD.
DR GO; GO:0090307; P:mitotic spindle assembly; ISO:RGD.
DR GO; GO:1905786; P:positive regulation of anaphase-promoting complex-dependent catabolic process; IBA:GO_Central.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IMP:RGD.
DR GO; GO:0045842; P:positive regulation of mitotic metaphase/anaphase transition; ISO:RGD.
DR GO; GO:0090129; P:positive regulation of synapse maturation; ISS:UniProtKB.
DR GO; GO:0031915; P:positive regulation of synaptic plasticity; ISS:UniProtKB.
DR GO; GO:1904668; P:positive regulation of ubiquitin protein ligase activity; ISS:UniProtKB.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0050773; P:regulation of dendrite development; IMP:RGD.
DR GO; GO:0040020; P:regulation of meiotic nuclear division; ISO:RGD.
DR CDD; cd00200; WD40; 1.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR InterPro; IPR024977; Apc4-like_WD40_dom.
DR InterPro; IPR033010; Cdc20/Fizzy.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR001680; WD40_rpt.
DR PANTHER; PTHR19918; CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED; 1.
DR PANTHER; PTHR19918:SF3; CELL DIVISION CYCLE PROTEIN 20 HOMOLOG; 1.
DR Pfam; PF12894; ANAPC4_WD40; 1.
DR Pfam; PF00400; WD40; 3.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; WD40 repeat-like; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 3.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cell cycle; Cell division; Centromere; Chromosome; Cytoplasm;
KW Cytoskeleton; Isopeptide bond; Kinetochore; Mitosis; Phosphoprotein;
KW Reference proteome; Repeat; Ubl conjugation; Ubl conjugation pathway;
KW WD repeat.
FT CHAIN 1..499
FT /note="Cell division cycle protein 20 homolog"
FT /id="PRO_0000050902"
FT REPEAT 182..221
FT /note="WD 1"
FT REPEAT 224..263
FT /note="WD 2"
FT REPEAT 266..303
FT /note="WD 3"
FT REPEAT 307..346
FT /note="WD 4"
FT REPEAT 353..395
FT /note="WD 5"
FT REPEAT 397..438
FT /note="WD 6"
FT REPEAT 441..480
FT /note="WD 7"
FT REGION 27..80
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 52..72
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 41
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q12834"
FT MOD_RES 66
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q12834"
FT MOD_RES 70
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q12834"
FT MOD_RES 72
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q12834"
FT MOD_RES 92
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q12834"
FT MOD_RES 106
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q12834"
FT MOD_RES 153
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q12834"
FT MOD_RES 157
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q12834"
FT MOD_RES 161
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q12834"
FT CROSSLNK 485
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0000250|UniProtKB:Q12834"
FT CROSSLNK 490
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0000250|UniProtKB:Q12834"
FT CONFLICT 77
FT /note="D -> E (in Ref. 1; AAA19018)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 499 AA; 54829 MW; 74B6261B57E40869 CRC64;
MAQFVFESDL HSLLQLDAPI PNAPIARWQR KAKEATGPAP SPMRAANRSH SAGRTPGRTP
GKSNSKVQTT PSKPGGDRYI PQRSASQMEV ASFLLSKENQ PEDGGTPTKK EHQKAWARNL
NGFDVEEAKI LRLSGKPQNA PEGYQNRLKV LYSQKATPGS SRKACRYIPS LPDRILDAPE
IRNDYYLNLV DWSSGNVLAV ALDNSVYLWN AGSGDILQLL QMEQPGDYIS SVAWIKEGNY
LAVGTSNAEV QLWDVQQQKR LRNMTSHSAR VSSLSWNSYI LSSGSRSGHI HHHDVRVAEH
HVATLSGHSQ EVCGLRWAPD GRHLASGGND NIVNVWPSGP GESGWVPLQT FTQHQGAVKA
VAWCPWQSNI LATGGGTSDR HIRIWNVCSG ACLSAVDVHS QVCSILWSPH YKELISGHGF
AQNQLVIWKY PTMAKVAELK GHTARVLSLT MSPDGATVAS AAADETLRLW RCFELDPALR
REREKASTSK SSLIHQGIR
//
