DiGAlign : the Dynamic Genomic Alignment server    version 2.0


DiGAlign is a genome alignment viewer that extends the alignment visualizing function implemented in ViPTree. Nucleotide- (BLASTn) or amino acid- (tBLASTx) resolved alignments of input nucleotide sequences are computed and interactively visualized to generate a publication-ready figure. Same as ViPTree, the alignment view can include gene and function predictions performed by the DiGAlign server or provided by the user. DiGAlign quickly and effectively assists you in visualizing the results as you want in comparative genomics and genome structure exploration.


Generate alignments of your query sequences.

  1. Upload your nucleotide sequences and choose the alignment and gene prediction options.
  2. Browse the alignments
  3. Browse dotplots / genomic alignments between your query sequences
  4. Download figures and tables


Here is an exmaple of the session main page (an entrance page after the computation).


Version history

  • 2023-11-17 version 2.0
    • Major update! Various functions are newly implemented, as listed below.
    • Gene prediction is now performed by Prodigal and any coding table is selectable if it is compatible with Prodigal.
    • In the alignment viewer, threshold for tBLASTx/BLASTn could be set.
    • In the alignment viewer, a mouseover popup is implemented that shows details of genes and tBLASTx/BLASTn hits.
    • A mouseover popup is implemented that shows details of genes and tBLASTx/BLASTn hits.
    • From this version, License is set as 'CC BY-NC-SA 4.0'
    • Many bug fixes, including the gene table upload function.
    • Document is updated.
  • 2022-04-18 version 1.0
    • Initial release of DiGAlign


If you use results (data / figures) generated by DiGAlign in your research, please cite the DiGAlign web site or the publication below:
  • Coming soon...


We appreciate bug reports, comments, and suggestions. The form is here.