ID A0A0H2Z457_ECOK1 Unreviewed; 614 AA.
AC A0A0H2Z457;
DT 16-SEP-2015, integrated into UniProtKB/TrEMBL.
DT 16-SEP-2015, sequence version 1.
DT 28-JAN-2026, entry version 47.
DE RecName: Full=Selenocysteine-specific elongation factor {ECO:0000256|ARBA:ARBA00015953};
DE AltName: Full=SelB translation factor {ECO:0000256|ARBA:ARBA00031615};
GN Name=selB {ECO:0000313|EMBL:ABJ03069.1};
GN ORFNames=APECO1_2863 {ECO:0000313|EMBL:ABJ03069.1};
OS Escherichia coli O1:K1 / APEC.
OC Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC Enterobacterales; Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=405955 {ECO:0000313|EMBL:ABJ03069.1, ECO:0000313|Proteomes:UP000008216};
RN [1] {ECO:0000313|EMBL:ABJ03069.1, ECO:0000313|Proteomes:UP000008216}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=APEC O1 {ECO:0000313|EMBL:ABJ03069.1};
RX PubMed=17293413; DOI=10.1128/JB.01726-06;
RA Johnson T.J., Kariyawasam S., Wannemuehler Y., Mangiamele P., Johnson S.J.,
RA Doetkott C., Skyberg J.A., Lynne A.M., Johnson J.R., Nolan L.K.;
RT "The genome sequence of avian pathogenic Escherichia coli strain O1:K1:H7
RT shares strong similarities with human extraintestinal pathogenic E. coli
RT genomes.";
RL J. Bacteriol. 189:3228-3236(2007).
CC -!- FUNCTION: Translation factor necessary for the incorporation of
CC selenocysteine into proteins. It probably replaces EF-Tu for the
CC insertion of selenocysteine directed by the UGA codon. SelB binds GTP
CC and GDP. {ECO:0000256|ARBA:ARBA00025526}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR EMBL; CP000468; ABJ03069.1; -; Genomic_DNA.
DR RefSeq; WP_000582432.1; NC_008563.1.
DR AlphaFoldDB; A0A0H2Z457; -.
DR SMR; A0A0H2Z457; -.
DR KEGG; ecv:APECO1_2863; -.
DR HOGENOM; CLU_023030_2_0_6; -.
DR Proteomes; UP000008216; Chromosome.
DR GO; GO:0005829; C:cytosol; IEA:TreeGrafter.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0035368; F:selenocysteine insertion sequence binding; IEA:TreeGrafter.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:InterPro.
DR GO; GO:0000049; F:tRNA binding; IEA:TreeGrafter.
DR GO; GO:0001514; P:selenocysteine incorporation; IEA:InterPro.
DR GO; GO:0016259; P:selenocysteine metabolic process; IEA:TreeGrafter.
DR CDD; cd04171; SelB; 1.
DR CDD; cd03696; SelB_II; 1.
DR CDD; cd15491; selB_III; 1.
DR FunFam; 1.10.10.10:FF:000350; Selenocysteine-specific translation elongation factor; 1.
DR FunFam; 2.40.30.10:FF:000080; Selenocysteine-specific translation elongation factor; 1.
DR FunFam; 3.40.50.300:FF:001064; Selenocysteine-specific translation elongation factor; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 2.40.30.10; Translation factors; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 2.
DR InterPro; IPR057335; Beta-barrel_SelB.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR050543; eIF2G.
DR InterPro; IPR015190; Elong_fac_SelB-wing-hlx_typ-2.
DR InterPro; IPR031157; G_TR_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR015191; SelB_WHD4.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR InterPro; IPR009001; Transl_elong_EF1A/Init_IF2_C.
DR InterPro; IPR004535; Transl_elong_SelB.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR InterPro; IPR048649; WHD_1st_3rd_SelB.
DR InterPro; IPR048931; WHD_2nd_SelB_bact.
DR NCBIfam; TIGR00475; selB; 1.
DR PANTHER; PTHR42854; EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3 FAMILY MEMBER; 1.
DR PANTHER; PTHR42854:SF3; EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3-RELATED; 1.
DR Pfam; PF25461; Beta-barrel_SelB; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR Pfam; PF21458; WHD_1st_3rd_SelB; 1.
DR Pfam; PF09106; WHD_2nd_SelB; 1.
DR Pfam; PF21214; WHD_2nd_SelB_bact; 1.
DR Pfam; PF09107; WHD_3rd_SelB; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF50465; EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF50447; Translation proteins; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 3.
DR PROSITE; PS00301; G_TR_1; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 4: Predicted;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW GTP-binding {ECO:0000256|ARBA:ARBA00023134};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000008216}.
FT DOMAIN 1..173
FT /note="Tr-type G"
FT /evidence="ECO:0000259|PROSITE:PS51722"
SQ SEQUENCE 614 AA; 68858 MW; 258BB67BB2797DB2 CRC64;
MIIATAGHVD HGKTTLLQAI TGVNADRLPE EKKRGMTIDL GYAYWPQPDG RVPGFIDVPG
HEKFLSNMLA GVGGIDHALL VVACDDGVMA QTREHLAILQ LTGNPMLTVA LTKADRVDEA
RVDEVERQVK EVLREYGFAE AKLFITAATE GRGIDALREH LLQLPEREHA SQHSFRLAID
RAFTVKGAGL VVTGTALSGE VKVGDSLWLT GVNKPMRVRA LHAQNQPTET AHAGQRIALN
IAGDAEKEQI NRGDWLLADV PPEPFTRVIV ELQTHTPLTQ WQPLHIHHAA SHVTGRVSLL
EDNLAELVFD TPLWLADNDR LVLRDISARN TLAGARVVML NPPRRGKRKP EYLQWLASLA
RAQSDADALS VHLERGAVNL ADFAWARQLN GEGMRELLQQ PGYIQAGYSL LNAPVAARWQ
RKILDTLATY HEQHRDEPGP GRERLRRMAL PMEDDALVLL LIEKMRESGD IHSHHGWLHL
PDHKAGFSEE QQAIWQKAEP LFGDEPWWVR DLAKETGTDE QAMRLTLRQA AQQGIITAIV
KDRYYRNDRI VEFANMIRDL DQECGSTCAA DFRDRLGVGR KLAIQILEYF DRIGFTRRRG
NDHLLRDALL FPEK
//
