ID A0A162C8N0_9CRUS Unreviewed; 1542 AA.
AC A0A162C8N0;
DT 06-JUL-2016, integrated into UniProtKB/TrEMBL.
DT 06-JUL-2016, sequence version 1.
DT 28-JAN-2026, entry version 35.
DE RecName: Full=RNA helicase aquarius {ECO:0000256|ARBA:ARBA00069875};
DE EC=3.6.4.13 {ECO:0000256|ARBA:ARBA00012552};
DE AltName: Full=Intron-binding protein of 160 kDa {ECO:0000256|ARBA:ARBA00083796};
GN ORFNames=APZ42_022053 {ECO:0000313|EMBL:KZS12822.1};
OS Daphnia magna.
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Branchiopoda;
OC Diplostraca; Cladocera; Anomopoda; Daphniidae; Daphnia.
OX NCBI_TaxID=35525 {ECO:0000313|EMBL:KZS12822.1, ECO:0000313|Proteomes:UP000076858};
RN [1] {ECO:0000313|EMBL:KZS12822.1, ECO:0000313|Proteomes:UP000076858}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Xinb3 {ECO:0000313|EMBL:KZS12822.1,
RC ECO:0000313|Proteomes:UP000076858};
RC TISSUE=Complete organism {ECO:0000313|EMBL:KZS12822.1};
RA Gilbert D.G., Choi J.-H., Mockaitis K., Colbourne J., Pfrender M.;
RT "EvidentialGene: Evidence-directed Construction of Genes on Genomes.";
RL Submitted (MAR-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in pre-mRNA splicing as component of the
CC spliceosome. Intron-binding spliceosomal protein required to link pre-
CC mRNA splicing and snoRNP (small nucleolar ribonucleoprotein)
CC biogenesis. Plays a key role in position-dependent assembly of intron-
CC encoded box C/D small snoRNP, splicing being required for snoRNP
CC assembly. May act by helping the folding of the snoRNA sequence. Binds
CC to intron of pre-mRNAs in a sequence-independent manner, contacting the
CC region between snoRNA and the branchpoint of introns (40 nucleotides
CC upstream of the branchpoint) during the late stages of splicing. Has
CC ATP-dependent RNA helicase activity and can unwind double-stranded RNA
CC molecules with a 3' overhang (in vitro).
CC {ECO:0000256|ARBA:ARBA00057313}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + phosphate + H(+); Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000256|ARBA:ARBA00047984};
CC -!- SUBUNIT: Identified in the spliceosome C complex. Component of the XAB2
CC complex, a multimeric protein complex composed of XAB2, PRPF19, AQR,
CC ZNF830, ISY1, and PPIE. Identified in a pentameric intron-binding (IB)
CC complex composed of AQR, XAB2, ISY1, ZNF830 and PPIE that is
CC incorporated into the spliceosome as a preassembled complex. The IB
CC complex does not contain PRPF19. Within the spliceosome, interacts with
CC SNRPA1, SF3B1, SF3B3, SF3A1 and SF3A2. {ECO:0000256|ARBA:ARBA00063921}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm
CC {ECO:0000256|ARBA:ARBA00004642}.
CC -!- SIMILARITY: Belongs to the CWF11 family.
CC {ECO:0000256|ARBA:ARBA00061244, ECO:0000256|PIRNR:PIRNR038901}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KZS12822.1}.
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DR EMBL; LRGB01001348; KZS12822.1; -; Genomic_DNA.
DR STRING; 35525.A0A162C8N0; -.
DR OrthoDB; 1879at2759; -.
DR Proteomes; UP000076858; Unassembled WGS sequence.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; IEA:TreeGrafter.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0003729; F:mRNA binding; IEA:TreeGrafter.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IEA:InterPro.
DR CDD; cd17935; EEXXQc_AQR; 1.
DR CDD; cd18808; SF1_C_Upf1; 1.
DR FunFam; 3.40.50.300:FF:000396; RNA helicase aquarius; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR048966; Aquarius_b-barrel.
DR InterPro; IPR048967; Aquarius_insert.
DR InterPro; IPR032174; Aquarius_N.
DR InterPro; IPR026300; CWF11_fam.
DR InterPro; IPR045055; DNA2/NAM7-like.
DR InterPro; IPR041679; DNA2/NAM7-like_C.
DR InterPro; IPR041677; DNA2/NAM7_AAA_11.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047187; SF1_C_Upf1.
DR PANTHER; PTHR10887; DNA2/NAM7 HELICASE FAMILY; 1.
DR PANTHER; PTHR10887:SF5; RNA HELICASE AQUARIUS; 1.
DR Pfam; PF13086; AAA_11; 2.
DR Pfam; PF13087; AAA_12; 1.
DR Pfam; PF16399; Aquarius_N_1st; 1.
DR Pfam; PF21143; Aquarius_N_2nd; 1.
DR Pfam; PF21144; Aquarius_N_3rd; 1.
DR PIRSF; PIRSF038901; AQR_cwf11; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW Acetylation {ECO:0000256|ARBA:ARBA00022990};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|SAM:Phobius};
KW mRNA processing {ECO:0000256|ARBA:ARBA00022664,
KW ECO:0000256|PIRNR:PIRNR038901};
KW mRNA splicing {ECO:0000256|ARBA:ARBA00023187,
KW ECO:0000256|PIRNR:PIRNR038901};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|PIRNR:PIRNR038901};
KW Reference proteome {ECO:0000313|Proteomes:UP000076858};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884};
KW Spliceosome {ECO:0000256|ARBA:ARBA00022728};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 1051..1071
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 31..421
FT /note="RNA helicase aquarius N-terminal"
FT /evidence="ECO:0000259|Pfam:PF16399"
FT DOMAIN 500..665
FT /note="RNA helicase aquarius beta-barrel"
FT /evidence="ECO:0000259|Pfam:PF21143"
FT DOMAIN 713..791
FT /note="RNA helicase aquarius insertion"
FT /evidence="ECO:0000259|Pfam:PF21144"
FT DOMAIN 803..1058
FT /note="DNA2/NAM7 helicase helicase"
FT /evidence="ECO:0000259|Pfam:PF13086"
FT DOMAIN 1122..1173
FT /note="DNA2/NAM7 helicase helicase"
FT /evidence="ECO:0000259|Pfam:PF13086"
FT DOMAIN 1182..1373
FT /note="DNA2/NAM7 helicase-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF13087"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 735..762
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1452..1542
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..14
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 735..754
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1471..1482
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1497..1515
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1516..1527
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1542 AA; 178596 MW; 424E9B76129CF4C6 CRC64;
MESKNRNNAS TGKNNENKIP LTLDQINSDR VTQVASKYWA PQTIGNHMEF DGDIVDQIYQ
EEIQGARFMI RRIMMLEFSQ YLENYLWPNY RPGSSSAYHV LSIVIMVNEK FRERVSAWQI
FQKLPENFPS FFKQLMEMSL DEKWGLTFKE RTIILIFFNH CFNSLEIDLI RDQVQKLVSL
SIWTCLPEGR REQELNSFPK LKKFWKVIQK KDAKENEESR TKIEFERRFL QRLMQKFLSL
LATIPLEGDI DIEAVHYCER FLELLIDLEA LLPTRRFFNV VMDDCHLVTR CHLSSLNHRS
EGKLFAQLLD MLKFYAHFEI NDQTGDPLTD RDMMQLHYDR ITALQKAAFV KYSELRRFAL
TNVASINTRE ALTKHFDGLS TTVLQDVAAY LKLLPPPDNS DKDEKDKQFL MELLISRHER
RASQLEALNE MPLYPTEDII WNENIVPTEY FSGEGCLALP KLNLQFLTLH DYLLRNFNLF
RLESTYEIRQ DIEDSVFRMK PWKGEDGNVV FGGWARMAQP ITAFSVVEVG KPNIGEKQPS
RVRADVSVIL DVRNDVKHEW ENLRKHDACF LLTVRPPGPP GTHFNFREPF VPQVGLVYVR
GCEVEGMLDH LGRIIEEGPD PRPKIPGGNR TFRVWLDCNQ YKLDMDRVSQ NGEDVYDTFN
ILMRRKPKEN NFKAVLETIR DLMNTDCVVP EWLHDIILGY GDPGAAHYSK MPNQIPALDF
NDTFLSYAHL KESFPDHEIR TNEEDGKAEE KEGGQDGPTK LVPPFRLTFN LQGKDILVEP
HVIPSRGPYP FNEPKKNAVR FTPTQIEAIR SGMQPGLTMV VGPPGTGKTD VAVQIISNIY
HNFPEQRTLI VTHSNMALNQ LFEKIMALDI DERHLLRMGH GEEELETEKD FSRYGRVNYV
LAKRLELLDQ VQRLQQSLGV TGDVAYTCET AGHFFRLHIL ARWEEYLARV RSDKSSGVTV
EDVGRFFPFA KFFDDAPQPI FKGCSYVEDM EIADGCWRFI RKIFSQLEEF RAFELLRSGL
DRSRYLLVRE AKIIAMTCTH AALKRRELVE LGFKIIIIYL FMFFVVXXXX XXXXXXXXXX
XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXYDNILMEE AAQILEIETF
IPLLLQTPQD GFNRLKRWIM IGDHHQLPPV IKNMAFQKYS NMEQSLFTRV VRLGVPTVDL
DAQGRARPSL CSLYQWRYKK LGNLEHVTSG PEYRVANTGF VYDFQLINVD DFQGVGESEP
NPYFFQNLAE AEYVVAVFMY MRLLGYPAEK ISILTTYNGQ KHLIRDVVNT RCATNPLIGR
PSKIDTVDKY QGQQNDYILL SLVKTRAVGH LRDVRRLVVA TSRARLGLYV FARASLFANC
FELTPTFRSL LQRPTDLRLL PEETYRTERL STEEPDEDAA MVINDMPQMA TFVYDLYRKK
VEAIAKDPNA EKPWAKPIEP MQEDVKQPVA EESDSESSEA EVEEVRIINE IEEEEEASGR
ETRQEPVKKQ KKNEEEKEEE DEKEEETATV ASTQEDQDSL RK
//
