ID A0A328E6S9_9ASTE Unreviewed; 1000 AA.
AC A0A328E6S9;
DT 10-OCT-2018, integrated into UniProtKB/TrEMBL.
DT 10-OCT-2018, sequence version 1.
DT 28-JAN-2026, entry version 33.
DE RecName: Full=RNA helicase {ECO:0000256|ARBA:ARBA00012552};
DE EC=3.6.4.13 {ECO:0000256|ARBA:ARBA00012552};
GN ORFNames=DM860_015442 {ECO:0000313|EMBL:RAL53714.1};
OS Cuscuta australis.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Convolvulaceae; Cuscuteae; Cuscuta;
OC Cuscuta subgen. Grammica; Cuscuta sect. Cleistogrammica.
OX NCBI_TaxID=267555 {ECO:0000313|EMBL:RAL53714.1, ECO:0000313|Proteomes:UP000249390};
RN [1] {ECO:0000313|EMBL:RAL53714.1, ECO:0000313|Proteomes:UP000249390}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Yunnan {ECO:0000313|Proteomes:UP000249390};
RC TISSUE=Vines {ECO:0000313|EMBL:RAL53714.1};
RA Liu H.;
RT "The Genome of Cuscuta australis (Dodder) Provides Insight into the
RT Evolution of Plant Parasitism.";
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + phosphate + H(+); Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000256|ARBA:ARBA00047984};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}. Plastid
CC {ECO:0000256|ARBA:ARBA00004474}.
CC -!- SIMILARITY: Belongs to the DExH box helicase family. SKI2 subfamily.
CC {ECO:0000256|ARBA:ARBA00061045}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RAL53714.1}.
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DR EMBL; NQVE01000017; RAL53714.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A328E6S9; -.
DR Proteomes; UP000249390; Unassembled WGS sequence.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-ARBA.
DR GO; GO:0009536; C:plastid; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0000460; P:maturation of 5.8S rRNA; IEA:TreeGrafter.
DR GO; GO:0006401; P:RNA catabolic process; IEA:InterPro.
DR CDD; cd18024; DEXHc_Mtr4-like; 1.
DR CDD; cd18795; SF2_C_Ski2; 1.
DR FunFam; 3.40.50.300:FF:000083; ATP-dependent RNA helicase DOB1; 1.
DR FunFam; 3.40.50.300:FF:000141; ATP-dependent RNA helicase DOB1; 1.
DR FunFam; 1.10.3380.30:FF:000006; DExH-box ATP-dependent RNA helicase DExH10; 1.
DR FunFam; 2.40.30.300:FF:000001; Mtr4 exosome RNA helicase; 1.
DR Gene3D; 1.10.3380.30; -; 1.
DR Gene3D; 2.40.30.300; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR025696; Beta-barrel_MTR4.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C-like.
DR InterPro; IPR048392; MTR4-like_stalk.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR050699; RNA-DNA_Helicase.
DR InterPro; IPR016438; SKI2-like.
DR InterPro; IPR012961; Ski2/MTR4_C.
DR PANTHER; PTHR12131; ATP-DEPENDENT RNA AND DNA HELICASE; 1.
DR PANTHER; PTHR12131:SF7; EXOSOME RNA HELICASE MTR4; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF08148; DSHCT; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF21408; MTR4-like_stalk; 1.
DR Pfam; PF13234; MTR4_beta-barrel; 1.
DR PIRSF; PIRSF005198; Antiviral_helicase_SKI2; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM01142; DSHCT; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000249390}.
FT DOMAIN 90..246
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 323..524
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT REGION 1..38
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 296..315
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1000 AA; 112271 MW; 9C47F764FCD4B7A7 CRC64;
MDVKAEEFSS QVAKRKYAEE NSAGVEVPDE DSASKRRNIT RTCVHEVAVP TGYTSSKDES
VHGTLSSPVY NGEMAKTYAF NLDPFQEVSV ACLERNESVL VSAHTSAGKT AVAEYAIAMS
FRDKQRVIYT SPLKALSNQK YRELSQEFSD VGLMTGDVTL SPNASCLVMT TEILRGMLYR
GSEVLKEVAW VIFDEIHYMK DRERGVVWEE SIIFLPPAIK MVFLSATMSN ATEFAEWICN
LHKQPCHVVY TDFRPTPLQH YVFPTGGSGL YLVVDETEKF REDNFAKVQD AFTKQHPANG
SKGINPKASG RIAKGGKASG GSDIYKIVKM IMERKFQPVI IFSFSRRECE QHAMSMSKLD
FNTEEEKDIV EQVFRNAVLC LNEEDRSLPA IELMLPLLQR GIAVHHSGLL PIIKELVELL
FQEGLVKALF ATETFAMGLN MPAKTVVFTS VKKWDGDSHR FIGSGEYIQM SGRAGRRGKD
DRGICIIMID EQMEMNTLKD MILGKPAPLL STFRLSYYSI LNLMSRAEGQ FTAERVIKHS
FHQFQYEKAL PDIGKKVSML EEEAATLDAS GEAEVAEYHK LKLEISQIEK KMMAEITRPE
RVLYFLLPGR LVKVREGGKD WGWGVVVNVV RRPPTTSGSL PAALSALRGS TYIVDTLLHC
SLGSSESGSR PKPCPPVPGE KGEMHVVPVQ LPLISAISKL RVTVPPDLRP LEARQSILLA
LGALEKSFPQ GFPKLNPVKD MEIEDPEFVD MMSQIEELEK KLFAHPLHKS QDEHQLKSFH
RKAEVNHEIQ QLKSKMRDSQ LQKFRDELKN RSRVLKKLGH IDADGVVQLK GRAACLIDTG
DELLVTELMF NGTFNDLDHH QIAALASCFI PGDKSNEQIH LRAELAKPLQ QLRESARRIA
EIQYECKLEI DVEEYVEACI RPFLMDVIYC WSKGASFAEV IQMTDIFEGS IIRLARRLDE
FLNQLKGAAH AVGEVGLESK FIAASESLRR GIMFANSLYL
//
