ID A0A7J7DLU4_TRIWF Unreviewed; 1750 AA.
AC A0A7J7DLU4;
DT 07-APR-2021, integrated into UniProtKB/TrEMBL.
DT 07-APR-2021, sequence version 1.
DT 18-JUN-2025, entry version 18.
DE RecName: Full=RNA helicase {ECO:0000256|ARBA:ARBA00012552};
DE EC=3.6.4.13 {ECO:0000256|ARBA:ARBA00012552};
GN ORFNames=HS088_TW05G00061 {ECO:0000313|EMBL:KAF5747340.1};
OS Tripterygium wilfordii (Thunder God vine).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Celastrales; Celastraceae; Tripterygium.
OX NCBI_TaxID=458696 {ECO:0000313|EMBL:KAF5747340.1, ECO:0000313|Proteomes:UP000593562};
RN [1] {ECO:0000313|EMBL:KAF5747340.1, ECO:0000313|Proteomes:UP000593562}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. XIE 37 {ECO:0000313|Proteomes:UP000593562};
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF5747340.1};
RX PubMed=32080175; DOI=10.1038/s41467-020-14776-1;
RA Tu L., Su P., Zhang Z., Gao L., Wang J., Hu T., Zhou J., Zhang Y., Zhao Y.,
RA Liu Y., Song Y., Tong Y., Lu Y., Yang J., Xu C., Jia M., Peters R.J.,
RA Huang L., Gao W.;
RT "Genome of Tripterygium wilfordii and identification of cytochrome P450
RT involved in triptolide biosynthesis.";
RL Nat. Commun. 11:971-971(2020).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + phosphate + H(+); Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000256|ARBA:ARBA00047984};
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH subfamily.
CC {ECO:0000256|ARBA:ARBA00008792}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAF5747340.1}.
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DR EMBL; JAAARO010000005; KAF5747340.1; -; Genomic_DNA.
DR FunCoup; A0A7J7DLU4; 871.
DR InParanoid; A0A7J7DLU4; -.
DR OrthoDB; 10009520at2759; -.
DR Proteomes; UP000593562; Chromosome 5.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:TreeGrafter.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR CDD; cd20335; BRcat_RBR; 1.
DR CDD; cd17917; DEXHc_RHA-like; 1.
DR CDD; cd22585; Rcat_RBR_DEAH12-like; 1.
DR CDD; cd18791; SF2_C_RHA; 1.
DR FunFam; 1.20.120.1750:FF:000020; ATP-dependent RNA helicase DEAH12 chloroplastic; 1.
DR FunFam; 3.40.50.300:FF:001279; ATP-dependent RNA helicase DEAH12 chloroplastic; 1.
DR FunFam; 3.40.50.300:FF:002114; ATP-dependent RNA helicase DEAH12 chloroplastic; 1.
DR FunFam; 1.20.120.1080:FF:000033; RBR-type E3 ubiquitin transferase; 1.
DR Gene3D; 1.20.120.1080; -; 1.
DR Gene3D; 1.20.120.1750; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR011709; DEAD-box_helicase_OB_fold.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR002464; DNA/RNA_helicase_DEAH_CS.
DR InterPro; IPR048333; HA2_WH.
DR InterPro; IPR007502; Helicase-assoc_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C-like.
DR InterPro; IPR002867; IBR_dom.
DR InterPro; IPR056245; KH_DEAH11/12.
DR InterPro; IPR056246; KH_DEAH11/12_1st.
DR InterPro; IPR056247; KH_DEAH11/12_2nd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR056248; RBD_DEAH11/12.
DR InterPro; IPR056244; RRM_DEAH11/12.
DR InterPro; IPR044066; TRIAD_supradom.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR18934; ATP-DEPENDENT RNA HELICASE; 1.
DR PANTHER; PTHR18934:SF81; ATP-DEPENDENT RNA HELICASE DEAH11, CHLOROPLASTIC-RELATED; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF04408; HA2_N; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF01485; IBR; 2.
DR Pfam; PF24471; KH_DEAH11; 1.
DR Pfam; PF24638; KH_DEAH11_1st; 1.
DR Pfam; PF24641; KH_DEAH11_2nd; 1.
DR Pfam; PF07717; OB_NTP_bind; 1.
DR Pfam; PF24475; RBD_DEAH11; 1.
DR Pfam; PF24637; RRM_DEAH11; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00847; HA2; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00647; IBR; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF57850; RING/U-box; 3.
DR PROSITE; PS00690; DEAH_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51873; TRIAD; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000313|EMBL:KAF5747340.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000593562};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00175}.
FT DOMAIN 294..458
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 491..654
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT DOMAIN 1537..1745
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS51873"
FT DOMAIN 1541..1587
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
FT REGION 1..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 41..50
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1750 AA; 197014 MW; 02416D1982AC78BC CRC64;
MKQSTGARRS PSPGYDPRFP PFQSRSVRPS TPPSFYPHQL YHQNRPQNQN NRYGQQFRSV
APNVCPECVV QLRASTGESS RSVKKDVDAL FSQCQSDHVR VVFSSSGSGS ATLYFQQWAE
ALSSLTKFWE LRLSGDHDLA PRLVSRAIDP DELKNRLRAL FVEHVKGIMD GEIVKRCQAK
IQLKSDEIAG VSAQLKERNK IWICNDLVQK RKGLSAERDL MGKRVKEFKE AMRCLLYYLG
EEDCAGVDMG SVKGVRVLKL KGEYDWERIY QLTARECRRL EDGLPIYAYR QQILYRIYEK
QTLVLIGETG SGKSTQLVQF LVDSGVAAEE SIVCTQPRKI AATSLTQRVR EESSGCYKDN
SVMCYPTFSS TQQFDSKVIY MTDHCLLQHY MNNRDLSGIS CIIIDEAHER SLNTDLVMAL
VRDLLFHKPN LRLVIMSATA NANQLANYFF GCEIFYVVGR NFPVDVRYVP CAIEESSSSK
IVSSYVVDVV NMAKEIHKTE KEGTILAFLT SQAEVEWACE KFEAPSAVAL PLHGKLSSEE
QFQVFQKFRG KRKVIFATNL AETSITIPGV KYVIDSGMVK ESKFDPNTGM NTLRVCWISQ
SSAEQRAGRA GRTEPGRCYR LYRKCDFESM PSNQEPEIRR VHLGVAVLRI MALGIKNVQE
FDFVDAPSTK AIDMAIKNLV QLGAIALNNG VFELTEDGRY MVKLGIEPRL GRLILSCAWH
RLAKEGLILA AVMANSSSIF CRVGNDEDKL RADGLKVQFC HPSGDLFTLL SVYKEWEAVA
PERRNIWCWD NSINAKSMRR CQDTAKELEY CLERELDFIV PTYWRWNPNK SSEHDECLKK
VILSALAENV AFYSGSDQLG YEVALNGQHV HLHPSCSLRI FGQKPTWVVF GEVLSTSGQY
LVCVTAFDFE YFSTLHPPPL FDVCKMEGRK LEVKVLTGFG ATLLKKFCGK SNNNLLSLRS
RIRAACMDER ICIEINFDQN EIQLFASKES MEKVTVIVVE VLECEKRWLS NECMEKYLYD
GHGFSPVALF GAGGEIKHLE LGKSALTVDV FHPSANMIDE KVLLLFFEKH TSGRVCSVHK
TSGIGQEGDE KEKWGRITFL SPDTAGKATE LNGVEFSGST LKVLPAPTSS GGNHRMAAFP
AIKAKIYWPR KRSKGFGVLK CHLNDIGYML DDFANVVMGG NYVRCEVSKR NDDCVVIYGI
DKELSEADIL GVLRTATHRR ILGFFLVRGD AVENPLHSTC EEALMREISS FMPKRNLYSS
CCRVQVFPPE PKDAFMKALM VFDGRLHLEA AKALEHLEGK VLPGCHSWQK LKCQQLFYSS
ISCPAYVYSV IKKQLDTVLA SFNYVKGAEY FLEKTESGSY RVRISANATR TVAELRRPLE
ELMRGKIIND ASLSPSLIQH LFSRDGFNLM KLLQRETGTC ILFDRRSLNV RVFGSSQNVA
KAEEKLIQSL LTHHESKQLE IRLRGGNLPP DLMKEVVKRF GPDLRGLEQK VPGVELTLNV
YRQVISFHGE RELRPKVEEI IHEIAQSSVG SDRSLDGENA CPICLCEVED GHRLEGCGHS
ACRQCLLDQL DSALKNLDSF PICCACEDCN IPILLTDLKS LLSSEKLEEL FRASLGAFVA
TSGGAYRFCP SPDCTSVYQV AEPGMGSVPF VCGACYAETC TRCHLECHPS VSCELYREFK
EDPDSSLKEW CRGKNNVKSC PVCGYTIEKT EGCNHVECLC GRHICWVCLD YYHSSEDCYG
HLRSVHMTFI
//
