ID A0A851J3U6_9PASS Unreviewed; 1035 AA.
AC A0A851J3U6;
DT 29-SEP-2021, integrated into UniProtKB/TrEMBL.
DT 29-SEP-2021, sequence version 1.
DT 28-JAN-2026, entry version 20.
DE RecName: Full=Exosome RNA helicase MTR4 {ECO:0000256|ARBA:ARBA00069430};
DE EC=3.6.4.13 {ECO:0000256|ARBA:ARBA00012552};
DE AltName: Full=Superkiller viralicidic activity 2-like 2 {ECO:0000256|ARBA:ARBA00082023};
DE AltName: Full=TRAMP-like complex helicase {ECO:0000256|ARBA:ARBA00077324};
DE Flags: Fragment;
GN Name=Mtrex {ECO:0000313|EMBL:NXB71442.1};
GN ORFNames=DONATR_R10143 {ECO:0000313|EMBL:NXB71442.1};
OS Donacobius atricapilla.
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Neoaves; Telluraves; Australaves;
OC Passeriformes; Mimidae; Donacobius.
OX NCBI_TaxID=237420 {ECO:0000313|EMBL:NXB71442.1, ECO:0000313|Proteomes:UP000660704};
RN [1] {ECO:0000313|EMBL:NXB71442.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=B10K-DU-001-63 {ECO:0000313|EMBL:NXB71442.1};
RC TISSUE=Muscle {ECO:0000313|EMBL:NXB71442.1};
RA Zhang G.;
RT "Bird 10,000 Genomes (B10K) Project - Family phase.";
RL Submitted (SEP-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + phosphate + H(+); Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000256|ARBA:ARBA00049390};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:13066;
CC Evidence={ECO:0000256|ARBA:ARBA00049390};
CC -!- SUBCELLULAR LOCATION: Nucleus speckle {ECO:0000256|ARBA:ARBA00004324}.
CC Nucleus, nucleolus {ECO:0000256|ARBA:ARBA00004604}. Nucleus,
CC nucleoplasm {ECO:0000256|ARBA:ARBA00004642}.
CC -!- SIMILARITY: Belongs to the helicase family. SKI2 subfamily.
CC {ECO:0000256|ARBA:ARBA00010140}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:NXB71442.1}.
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DR EMBL; WBMY01003098; NXB71442.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A851J3U6; -.
DR Proteomes; UP000660704; Unassembled WGS sequence.
DR GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0006974; P:DNA damage response; IEA:UniProtKB-KW.
DR GO; GO:0000460; P:maturation of 5.8S rRNA; IEA:TreeGrafter.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0006401; P:RNA catabolic process; IEA:InterPro.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR CDD; cd18024; DEXHc_Mtr4-like; 1.
DR CDD; cd13154; KOW_Mtr4; 1.
DR CDD; cd18795; SF2_C_Ski2; 1.
DR FunFam; 3.40.50.300:FF:000083; ATP-dependent RNA helicase DOB1; 1.
DR FunFam; 3.40.50.300:FF:000141; ATP-dependent RNA helicase DOB1; 1.
DR FunFam; 2.40.30.300:FF:000001; Mtr4 exosome RNA helicase; 1.
DR FunFam; 1.10.3380.30:FF:000002; superkiller viralicidic activity 2-like 2; 1.
DR FunFam; 1.10.3380.30:FF:000004; Superkiller viralicidic activity 2-like 2; 1.
DR Gene3D; 1.10.3380.30; -; 2.
DR Gene3D; 2.40.30.300; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR025696; Beta-barrel_MTR4.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C-like.
DR InterPro; IPR048392; MTR4-like_stalk.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR050699; RNA-DNA_Helicase.
DR InterPro; IPR016438; SKI2-like.
DR InterPro; IPR012961; Ski2/MTR4_C.
DR PANTHER; PTHR12131; ATP-DEPENDENT RNA AND DNA HELICASE; 1.
DR PANTHER; PTHR12131:SF7; EXOSOME RNA HELICASE MTR4; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF08148; DSHCT; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF21408; MTR4-like_stalk; 1.
DR Pfam; PF13234; MTR4_beta-barrel; 1.
DR PIRSF; PIRSF005198; Antiviral_helicase_SKI2; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM01142; DSHCT; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW Acetylation {ECO:0000256|ARBA:ARBA00022990};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000313|EMBL:NXB71442.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Isopeptide bond {ECO:0000256|ARBA:ARBA00022499};
KW mRNA processing {ECO:0000256|ARBA:ARBA00022664};
KW mRNA splicing {ECO:0000256|ARBA:ARBA00023187};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000660704};
KW rRNA processing {ECO:0000256|ARBA:ARBA00022552};
KW Spliceosome {ECO:0000256|ARBA:ARBA00022728};
KW Ubl conjugation {ECO:0000256|ARBA:ARBA00022843}.
FT DOMAIN 140..296
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 397..569
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT REGION 20..43
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 34..43
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:NXB71442.1"
FT NON_TER 1035
FT /evidence="ECO:0000313|EMBL:NXB71442.1"
SQ SEQUENCE 1035 AA; 116988 MW; 5CE7A325A733474A CRC64;
MAEAFGDELF SVFEEDAAAA ASGAKKNKTG PAEGARRSGK RLEEKPLVPA IVKPKRGVEI
DSTENVILGK KPKLEDVLSE DVNLTDLMPR VKVQSVDTVE GCTHEVALPA NEEFMGLKPR
TGKAAKEYPF ILDAFQREAI LCVDNNQSVL VSAHTSAGKT VCAEYAIALA LREKQRVIFT
SPIKALSNQK YREMYEEFQD VGLMTGDVTI NPTASCLVMT TEILRSMLYR GSEVMREVAW
VIFDEIHYMR DTERGVVWEE TIILLPDNVH YVFLSATIPN ARQFAEWICH LHKQPCHVIY
TDYRPTPLQH YIFPAGGDGL HLVVDENGDF REDNFNTAMQ VLRDAGDLAK GDQKGRKGGT
KGPSNVFKIV KMIMERNFQP VIIFSFSKKD CEAYALQMTK LDFNTDEEKK MVEEVFNNAI
DCLSDEDKKL PQVEHVLPLL KRGIGIHHGG LLPILKETIE ILFSEGLIKA LFATETFAMG
INMPARTVLF TSASKFDGKD FRWISSGEYI QMSGRAGRRG MDDRGIVILM VDEKMSPTIG
KQLLKGSADP LNSAFHLTYN MVLNLLRVEE INPEYMLEKS FYQFQHYRTI PEIVEKVNNL
EAQYNKIVIP NEENVVIYYR IRQQLAKLGK DIEEYIHKPK YCLPFLQPGR LVKVVKNEGD
DYGWGVVVNF SKKSNVKPNS GELDPLYVVE VLLHCSKDSL KNSATESAKP ARPDERGEMQ
VVPVLVHLVS AISSVRLYIP KDLRPTDNRQ SVLKSVLEVQ KRFPDGVPLL DPIDDMGIKD
QGLKKVIQKI EAFEHRMYSH PLHNDPNLEA IYKLCEKKAQ IAMDIKVAKR ELKKARTVLQ
MDELKCRKRV LRRLGFATSS DVIEMKGRVA CEISSADELL LTEMMFNGLF NDLTAEQATA
LLSCFVFQEN SSEMPKLTEQ LAGPLRQMQE CAKRIAKVSA EAKLETDEEN YLNSFRPNLM
DVVYTWANGA NFAHICKMTD VFEGSIIRCM RRLEELLRQM CQAAKAIGNT ELENKFAEGI
TKIKRDIVFA ASLYL
//
