ID Q4AC01_BRACM Unreviewed; 1755 AA.
AC Q4AC01; M4CPB9;
DT 13-SEP-2005, integrated into UniProtKB/TrEMBL.
DT 13-SEP-2005, sequence version 1.
DT 18-JUN-2025, entry version 108.
DE RecName: Full=RNA helicase {ECO:0000256|ARBA:ARBA00012552};
DE EC=3.6.4.13 {ECO:0000256|ARBA:ARBA00012552};
GN Name=117M18_19 {ECO:0000313|EMBL:AAZ66938.1,
GN ECO:0000313|EnsemblPlants:Bra006057.1-P};
OS Brassica campestris (Field mustard).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3711 {ECO:0000313|EMBL:AAZ66938.1};
RN [1] {ECO:0000313|EMBL:AAZ66938.1}
RP NUCLEOTIDE SEQUENCE.
RG KBGP;
RA Park B.S., Lee M.C., Kwon S.J., Kim J.S., Lee S.I., Lim G.B., Kim J.A.,
RA Jin Y.M., Kim D.H., Yang T.J., Kim H.I.;
RT "Brassica rapa clone BAC KbrH117M18, Phase II, ordered two contigs.";
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EnsemblPlants:Bra006057.1-P, ECO:0000313|Proteomes:UP000011750}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chiifu-401-42 {ECO:0000313|EnsemblPlants:Bra006057.1-P,
RC ECO:0000313|Proteomes:UP000011750};
RX PubMed=21873998; DOI=10.1038/ng.919;
RG Brassica rapa Genome Sequencing Project Consortium;
RA Wang X., Wang H., Wang J., Sun R., Wu J., Liu S., Bai Y., Mun J.H.,
RA Bancroft I., Cheng F., Huang S., Li X., Hua W., Wang J., Wang X.,
RA Freeling M., Pires J.C., Paterson A.H., Chalhoub B., Wang B., Hayward A.,
RA Sharpe A.G., Park B.S., Weisshaar B., Liu B., Li B., Liu B., Tong C.,
RA Song C., Duran C., Peng C., Geng C., Koh C., Lin C., Edwards D., Mu D.,
RA Shen D., Soumpourou E., Li F., Fraser F., Conant G., Lassalle G.,
RA King G.J., Bonnema G., Tang H., Wang H., Belcram H., Zhou H., Hirakawa H.,
RA Abe H., Guo H., Wang H., Jin H., Parkin I.A., Batley J., Kim J.S., Just J.,
RA Li J., Xu J., Deng J., Kim J.A., Li J., Yu J., Meng J., Wang J., Min J.,
RA Poulain J., Wang J., Hatakeyama K., Wu K., Wang L., Fang L., Trick M.,
RA Links M.G., Zhao M., Jin M., Ramchiary N., Drou N., Berkman P.J., Cai Q.,
RA Huang Q., Li R., Tabata S., Cheng S., Zhang S., Zhang S., Huang S.,
RA Sato S., Sun S., Kwon S.J., Choi S.R., Lee T.H., Fan W., Zhao X., Tan X.,
RA Xu X., Wang Y., Qiu Y., Yin Y., Li Y., Du Y., Liao Y., Lim Y., Narusaka Y.,
RA Wang Y., Wang Z., Li Z., Wang Z., Xiong Z., Zhang Z.;
RT "The genome of the mesopolyploid crop species Brassica rapa.";
RL Nat. Genet. 43:1035-1039(2011).
RN [3] {ECO:0000313|EnsemblPlants:Bra006057.1-P, ECO:0000313|Proteomes:UP000011750}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chiifu-401-42 {ECO:0000313|EnsemblPlants:Bra006057.1-P,
RC ECO:0000313|Proteomes:UP000011750};
RX PubMed=30131865;
RA Zhang L., Cai X., Wu J., Liu M., Grob S., Cheng F., Liang J., Cai C.,
RA Liu Z., Liu B., Wang F., Li S., Liu F., Li X., Cheng L., Yang W., Li M.H.,
RA Grossniklaus U., Zheng H., Wang X.;
RT "Improved Brassica rapa reference genome by single-molecule sequencing and
RT chromosome conformation capture technologies.";
RL Hortic Res 5:50-50(2018).
RN [4] {ECO:0000313|EnsemblPlants:Bra006057.1-P}
RP IDENTIFICATION.
RC STRAIN=cv. Chiifu-401-42 {ECO:0000313|EnsemblPlants:Bra006057.1-P};
RG EnsemblPlants;
RL Submitted (MAR-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + phosphate + H(+); Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000256|ARBA:ARBA00047984};
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH subfamily.
CC {ECO:0000256|ARBA:ARBA00008792}.
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DR EMBL; AC146875; AAZ66938.1; -; Genomic_DNA.
DR RefSeq; XP_009131152.1; XM_009132904.2.
DR RefSeq; XP_009131153.1; XM_009132905.2.
DR STRING; 51351.M4CPB9; -.
DR EnsemblPlants; Bra006057.1; Bra006057.1-P; Bra006057.
DR Gramene; Bra006057.1; Bra006057.1-P; Bra006057.
DR eggNOG; KOG0922; Eukaryota.
DR eggNOG; KOG1812; Eukaryota.
DR HOGENOM; CLU_001832_9_0_1; -.
DR OMA; KEWEAFP; -.
DR OrthoDB; 10009520at2759; -.
DR Proteomes; UP000011750; Chromosome A03.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IBA:GO_Central.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR CDD; cd20335; BRcat_RBR; 1.
DR CDD; cd17917; DEXHc_RHA-like; 1.
DR CDD; cd22585; Rcat_RBR_DEAH12-like; 1.
DR CDD; cd18791; SF2_C_RHA; 1.
DR FunFam; 1.20.120.1750:FF:000020; ATP-dependent RNA helicase DEAH12 chloroplastic; 1.
DR FunFam; 3.40.50.300:FF:001279; ATP-dependent RNA helicase DEAH12 chloroplastic; 1.
DR FunFam; 3.40.50.300:FF:002114; ATP-dependent RNA helicase DEAH12 chloroplastic; 1.
DR Gene3D; 1.20.120.1750; -; 1.
DR Gene3D; 1.10.10.2130; DEAH helicase family, winged-helix domain; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR011709; DEAD-box_helicase_OB_fold.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR042035; DEAH_win-hel_dom.
DR InterPro; IPR002464; DNA/RNA_helicase_DEAH_CS.
DR InterPro; IPR007502; Helicase-assoc_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C-like.
DR InterPro; IPR002867; IBR_dom.
DR InterPro; IPR056245; KH_DEAH11/12.
DR InterPro; IPR056246; KH_DEAH11/12_1st.
DR InterPro; IPR056247; KH_DEAH11/12_2nd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR056248; RBD_DEAH11/12.
DR InterPro; IPR056244; RRM_DEAH11/12.
DR InterPro; IPR044066; TRIAD_supradom.
DR InterPro; IPR013087; Znf_C2H2_type.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR PANTHER; PTHR18934; ATP-DEPENDENT RNA HELICASE; 1.
DR PANTHER; PTHR18934:SF81; ATP-DEPENDENT RNA HELICASE DEAH11, CHLOROPLASTIC-RELATED; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF01485; IBR; 1.
DR Pfam; PF24471; KH_DEAH11; 1.
DR Pfam; PF24638; KH_DEAH11_1st; 1.
DR Pfam; PF24641; KH_DEAH11_2nd; 1.
DR Pfam; PF07717; OB_NTP_bind; 1.
DR Pfam; PF24475; RBD_DEAH11; 1.
DR Pfam; PF24637; RRM_DEAH11; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00847; HA2; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00647; IBR; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF57850; RING/U-box; 3.
DR PROSITE; PS00690; DEAH_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51873; TRIAD; 1.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000011750};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771}.
FT DOMAIN 285..449
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 480..645
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT DOMAIN 1532..1740
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS51873"
FT REGION 1..53
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 13..30
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1755 AA; 197510 MW; 8625B1D2E1EF3BE6 CRC64;
MKSSLPPAYR GRSATNRHPS LPSASTNCRN FRSPPLNHRQ YPSSSFPPNY RRDRPPATPN
FIVQLVHPRT PAAEPSPSFS VRKQVISTLA SLCGIPEESV HVPQFGCIAG SFSFRQWVDA
LSAVVALWEY RLQGKTLLVP ELVANVTVPS DMEELRDRLR GLFSGHVLSI LDNGDCVKKV
RAEIEEKTRQ VESFSSKRGI KLEAFERKKA IEAERDLVVK RLEEFKNGMK SIVRFLEGRD
GEKDDVAVFS LEGDYDWPRI HSLIRRECRR LEDGLPIYAY RRNILKRIHG EQVMVLIGET
GSGKSTQLVQ FLADSGVAAT ESIVCTQPRK IAALTLADRV REESNGCYEE NSVRCTPAFS
STEEISSKVV FMTDNCLLQH YIKDRSLPGV SCVIIDEAHE RSLNTDLLLA LLKDLMCRRI
DLRLVIMSAT ADAYQLSDYF FGCGILCVTG RNFPVEIIYS PSDAEGSSGT GRIAPYADDV
VKKAVEVHKT EKEGTILAFL TSQAEVEWAC ERFIAPSAVA LPLHGKLSFE EQFRVFQNHP
GKRKVIFATN IAETSLTIPG VKYVIDSGMV KESKYEPRTG MSILRVCRVS QSSARQRAGR
AGRTEPGRCY RLYSKHEFES MNLNQEPEIR RVHLGVALLR ILALGVDSVA DFDFVDAPVP
EAIAMAVKNL VQLGAVVEKN GVLELTQEGH CLVKLGLEPK LGKLILGCFR HRMGKEGIVL
AAVMANASSI FCRVGSLDDK MKADCLKVQF CNPNGDLFTL LSVYKEWASL PRERRNKWCW
ENSLNAKSMR RCEDTVKELE ICIERELSLV SPSYWVWNPT EGNNNKHDKR LKTVILASLA
ENVAMYTGYD QLGYEVALTG QQVQLHPSCS LLAFGQKPTW VVFGELLSIV DQYLVCVTSF
DFEALSLLDP PPPFDASHMD EWRLRVEKVA GCSSTLLKRF CGKSNCGLLS IVSRARLLCV
DERISIQVDV DQNEILLYAP PHDMDKVSAL VSDALECEKK WMRNECLEKY IYHGRGQVPM
ALFGSGAQIK HLEVDQRFLT ADVFYYGDSF VDDRELLRFL ERKIDVCICS IHKFAGNKQD
CDEKDKWGRI TFLTPESAMK ATEIQRFDFN GSVLKLFPSL ATGGGGMFKM PSFPSVTAMI
RWPQRESTGR GVLKCPSGDV HSVFNGTSDI VIGTRYARFE TDQRFSDSVL ITNIDSGLVE
ADVLDALEFY TGRTDLEFFL LRRKSVRSPP PTVCADMLHK RIFACMSSSS PEPNCVQVKV
FEPKETDYFM RALITFDGRL HLEAAKALEE LDGEVLPGCL SWQKIKCEQK FQSSIICPAS
VYNIVKRQLN AKLTSFERQR GGTWHLEPTH GGAYRVRISA YATKPISEMR RSLEELVRGR
PINHPGLTPR VLQHLSSRDG VILMRRIQHE TETYILLDRH SLTVRICGSE EKIAAAEQEL
VRSLLVYHER QQLEIRLRGP QLRPDLMKEV VNRFGPDLQG IKEKVNGVDL KLNTRYHVIQ
VHGSVEMRQE VEKIVYELAR EGSEPGGKPD DIEVECPICL CEVDDGYSLE GCSHLFCKAC
LLEQLEASMR NFDAFPILCS HTDCGAPIVL ADMRALLSQE KLDELFKASL SSFVTTSDGN
FRFCSTPDCP SVYRVAVGPR ESGEPFICGA CNAETCRRCH LEYHPYITCE RYKLFKEDPD
MSLKDWAKGK NVKECPFCKS TIEKSDGCNH LLCRCGKHIC WVCLDFFTHQ EPCYEHLRAV
HGGIGIFDEL GIPLL
//
