+D
#
Transfer RNA Biogenesis - Photobacterium profundum
%
!
AEukaryotic type
B 5'processing factors
C Ribonucrease P
B 3'processing and CCA adding factors
C 3'processing factors
D PBPRA2046 hypothetical ribonuclease K01166 RNASET2; ribonuclease T2 [EC:4.6.1.19]
C CCA adding factors
D PBPRA0439 SF3097; putative tRNA nucleotidyltransferase K00974 cca; tRNA nucleotidyltransferase (CCA-adding enzyme) [EC:2.7.7.72 3.1.3.- 3.1.4.-]
B tRNA spricing factors
C tRNA splicing endonuclease complex
C tRNA splicing ligase complex
C Other tRNA splicing factors
B tRNA transport factors
C tRNA export factors
C tRNA import factors
B Aminoacyl-tRNA synthetases (AARSs)
C Multi-aminoacyl-tRNA synthetase complex (MSC)
D PBPRA0874 putative glutamyl-tRNA synthetase K01885 EARS; glutamyl-tRNA synthetase [EC:6.1.1.17]
D PBPRA2944 PROS; putative prolyl-tRNA synthetase K01881 PARS; prolyl-tRNA synthetase [EC:6.1.1.15]
D PBPRA0591 ECS0029; putative Isoleucyl-tRNA synthetase K01870 IARS; isoleucyl-tRNA synthetase [EC:6.1.1.5]
D PBPRA2885 S0661; putative leucyl-tRNA synthetase K01869 LARS; leucyl-tRNA synthetase [EC:6.1.1.4]
D PBPRA1168 METG; putative methionyl-tRNA synthetase K01874 MARS; methionyl-tRNA synthetase [EC:6.1.1.10]
D PBPRA1034 S0624; putative glutaminyl-tRNA synthetase K01886 QARS; glutaminyl-tRNA synthetase [EC:6.1.1.18]
D PBPRA1064 putative arginyl-tRNA synthetase K01887 RARS; arginyl-tRNA synthetase [EC:6.1.1.19]
D PBPRA0570 putative lysyl-tRNA synthetase K04567 KARS; lysyl-tRNA synthetase, class II [EC:6.1.1.6]
D PBPRA1112 SF1876; Putative aspartyl-tRNA synthetase K01876 DARS2; aspartyl-tRNA synthetase [EC:6.1.1.12]
C Other AARSs
D PBPRA3067 putative alanyl-tRNA synthetase K01872 AARS; alanyl-tRNA synthetase [EC:6.1.1.7]
D PBPRA0768 VV0771; hypothetical metal-dependent hydrolase K01872 AARS; alanyl-tRNA synthetase [EC:6.1.1.7]
D PBPRA2335 ASNS; putative asparaginyl-tRNA synthetase K01893 NARS; asparaginyl-tRNA synthetase [EC:6.1.1.22]
D PBPRA2159 THRS; putative tRNA synthetase K01868 TARS; threonyl-tRNA synthetase [EC:6.1.1.3]
D PBPRA2160 PLU2669; hypothetical protein K01868 TARS; threonyl-tRNA synthetase [EC:6.1.1.3]
D PBPRA2161 PLU2669; putative threonyl-tRNA synthetase K01868 TARS; threonyl-tRNA synthetase [EC:6.1.1.3]
D PBPRA1165 SERS; Putative seryl-tRNA synthetase K01875 SARS; seryl-tRNA synthetase [EC:6.1.1.11]
D PBPRA1098 S0465; putative cysteinyl-tRNA synthetase K01883 CARS; cysteinyl-tRNA synthetase [EC:6.1.1.16]
D PBPRB1226 hypothetical cysteinyl-tRNA synthetase (cysS) K01883 CARS; cysteinyl-tRNA synthetase [EC:6.1.1.16]
D PBPRA0483 ECS5235; putative Valyl-tRNA synthetase K01873 VARS; valyl-tRNA synthetase [EC:6.1.1.9]
D PBPRA0764 putative histidyl-tRNA synthetase K01892 HARS; histidyl-tRNA synthetase [EC:6.1.1.21]
D PBPRA2156 Putative phenylalanyl-tRNA synthetase, alpha chain K01889 FARSA; phenylalanyl-tRNA synthetase alpha chain [EC:6.1.1.20]
D PBPRA2155 Putative phenylalanyl-tRNA synthetase, beta chain K01890 FARSB; phenylalanyl-tRNA synthetase beta chain [EC:6.1.1.20]
D PBPRA0533 PA0668; putative tyrosyl-tRNA synthetase K01866 YARS; tyrosyl-tRNA synthetase [EC:6.1.1.1]
D PBPRA0286 putative tryptophanyl-tRNA synthetase K01867 WARS; tryptophanyl-tRNA synthetase [EC:6.1.1.2]
D PBPRB0397 TRPS; putative tryptophanyl-tRNA synthetase K01867 WARS; tryptophanyl-tRNA synthetase [EC:6.1.1.2]
C Trans-editing factors
D PBPRA3488 putative D-tyrosyl-tRNA(Tyr) deacylase K07560 dtd; D-aminoacyl-tRNA deacylase [EC:3.1.1.96]
C AARS associated factors
D PBPRA2403 Y1770; putative Hit, Diadenosine tetraphosphate (Ap4A) hydrolase and other HIT family hydrolases K02503 HINT1_2; histidine triad (HIT) family protein [EC:3.9.1.-]
B tRNA modification factors
C Deaminases
C Dihydorouridine synthases
C Methylthiotransferase
C Methyltransferases
D PBPRA3152 VV2883; putative S-adenosylmethionine-dependent methyltransferase K03439 trmB; tRNA (guanine-N7-)-methyltransferase [EC:2.1.1.33]
D PBPRA2943 ECS0197; conserved hypothetical protein K22900 TRMO; tRNA (adenine37-N6)-methyltransferase [EC:2.1.1.-]
C Psudouridine synthases
D PBPRA2653 Putative tRNA pseudouridine synthase A K06173 truA; tRNA pseudouridine38-40 synthase [EC:5.4.99.12]
D PBPRA0614 EBIG1690; putative tRNA pseudouridine 5S synthase K03177 truB; tRNA pseudouridine55 synthase [EC:5.4.99.25]
D PBPRA3075 hypothetical hydrogenase subunit K06176 truD; tRNA pseudouridine13 synthase [EC:5.4.99.27]
C Thiolation factors
D PBPRA0750 PLU3283; Putative aminotransferase NifS, class V K04487 iscS; cysteine desulfurase [EC:2.8.1.7]
C KEOPS/EKC complex
C Elongator complex
C Elongator related proteins
C Other tRNA modification factors
D PBPRA3573 YRDC; Putative Sua5/YciO/YrdC family protein K07566 tsaC; L-threonylcarbamoyladenylate synthase [EC:2.7.7.87]
D PBPRA3351 VV3072; putative tRNA delta(2)-isopentenylpyrophosphate transferase K00791 miaA; tRNA dimethylallyltransferase [EC:2.5.1.75]
D PBPRA0002 S4013; Putative thiophene and furan oxidation protein ThdF K03650 mnmE; tRNA modification GTPase [EC:3.6.-.-]
D PBPRA3615 S3947; Putative glucose inhibited division protein A K03495 gidA; tRNA uridine 5-carboxymethylaminomethyl modification enzyme
D PBPRA2592 PLU2551; Putative selenophosphate synthase K01008 selD; selenide, water dikinase [EC:2.7.9.3]
D PBPRB1545 EF2567; Hypothetical selenophosphate synthase K01008 selD; selenide, water dikinase [EC:2.7.9.3]
B tRNA degradation factors
C TRAMP complex
C Rapid tRNA decay (RTD) pathway factors
D PBPRA3307 CYSQ-2; putative sulfite synthesis pathway protein K01082 cysQ; 3'(2'), 5'-bisphosphate nucleotidase [EC:3.1.3.7]
D PBPRA3469 XCC2882; putative CysQ protein K01082 cysQ; 3'(2'), 5'-bisphosphate nucleotidase [EC:3.1.3.7]
C Other tRNA degradation factors
#
AProkaryotic type
B 5'processing factors
C Ribonucrease P
D PBPRA0004 RNPA; Putative RNase P protein component K03536 rnpA; ribonuclease P protein component [EC:3.1.26.5]
B 3'processing and CCA adding factors
C 3'processing factors
D PBPRA2572 SO2755; putative ribonuclease T K03683 rnt; ribonuclease T [EC:3.1.13.-]
D PBPRA0198 S4086; putative ribonuclease PH K00989 rph; ribonuclease PH [EC:2.7.7.56]
D PBPRB0426 putative exoribonuclease II K01147 rnb; exoribonuclease II [EC:3.1.13.1]
D PBPRA1076 RND; hypothetical ribonuclease D K03684 rnd; ribonuclease D [EC:3.1.13.5]
D PBPRA2046 hypothetical ribonuclease K01166 RNASET2; ribonuclease T2 [EC:4.6.1.19]
C CCA adding factors
D PBPRA0439 SF3097; putative tRNA nucleotidyltransferase K00974 cca; tRNA nucleotidyltransferase (CCA-adding enzyme) [EC:2.7.7.72 3.1.3.- 3.1.4.-]
B tRNA spricing factors
C tRNA splicing endonucleases
C tRNA splicing ligases
C Other tRNA splicing factors
D PBPRA3169 hypothetical protein K01975 thpR; RNA 2',3'-cyclic 3'-phosphodiesterase [EC:3.1.4.58]
B tRNA transport factors
C tRNA export factors
D PBPRA0640 STM4581; putative ABC transporter, ATP-binding protein K06020 ettA; energy-dependent translational throttle protein EttA
B Aminoacyl-tRNA synthetases (AARSs)
C Multi-aminoacyl-tRNA synthetase complex (MSC)
D PBPRA2885 S0661; putative leucyl-tRNA synthetase K01869 LARS; leucyl-tRNA synthetase [EC:6.1.1.4]
D PBPRA2944 PROS; putative prolyl-tRNA synthetase K01881 PARS; prolyl-tRNA synthetase [EC:6.1.1.15]
C Other AARSs
D PBPRA0533 PA0668; putative tyrosyl-tRNA synthetase K01866 YARS; tyrosyl-tRNA synthetase [EC:6.1.1.1]
D PBPRA0286 putative tryptophanyl-tRNA synthetase K01867 WARS; tryptophanyl-tRNA synthetase [EC:6.1.1.2]
D PBPRB0397 TRPS; putative tryptophanyl-tRNA synthetase K01867 WARS; tryptophanyl-tRNA synthetase [EC:6.1.1.2]
D PBPRA2159 THRS; putative tRNA synthetase K01868 TARS; threonyl-tRNA synthetase [EC:6.1.1.3]
D PBPRA2160 PLU2669; hypothetical protein K01868 TARS; threonyl-tRNA synthetase [EC:6.1.1.3]
D PBPRA2161 PLU2669; putative threonyl-tRNA synthetase K01868 TARS; threonyl-tRNA synthetase [EC:6.1.1.3]
D PBPRA0591 ECS0029; putative Isoleucyl-tRNA synthetase K01870 IARS; isoleucyl-tRNA synthetase [EC:6.1.1.5]
D PBPRA0570 putative lysyl-tRNA synthetase K04567 KARS; lysyl-tRNA synthetase, class II [EC:6.1.1.6]
D PBPRA3067 putative alanyl-tRNA synthetase K01872 AARS; alanyl-tRNA synthetase [EC:6.1.1.7]
D PBPRA0768 VV0771; hypothetical metal-dependent hydrolase K01872 AARS; alanyl-tRNA synthetase [EC:6.1.1.7]
D PBPRA0483 ECS5235; putative Valyl-tRNA synthetase K01873 VARS; valyl-tRNA synthetase [EC:6.1.1.9]
D PBPRA1168 METG; putative methionyl-tRNA synthetase K01874 MARS; methionyl-tRNA synthetase [EC:6.1.1.10]
D PBPRA1165 SERS; Putative seryl-tRNA synthetase K01875 SARS; seryl-tRNA synthetase [EC:6.1.1.11]
D PBPRA1112 SF1876; Putative aspartyl-tRNA synthetase K01876 DARS2; aspartyl-tRNA synthetase [EC:6.1.1.12]
D PBPRA0056 GLYQ; Putative glycyl-tRNA synthetase, alpha chain K01878 glyQ; glycyl-tRNA synthetase alpha chain [EC:6.1.1.14]
D PBPRA0055 GLYS; Putative glycyl-tRNA synthetase, beta chain K01879 glyS; glycyl-tRNA synthetase beta chain [EC:6.1.1.14]
D PBPRA1098 S0465; putative cysteinyl-tRNA synthetase K01883 CARS; cysteinyl-tRNA synthetase [EC:6.1.1.16]
D PBPRB1226 hypothetical cysteinyl-tRNA synthetase (cysS) K01883 CARS; cysteinyl-tRNA synthetase [EC:6.1.1.16]
D PBPRA0874 putative glutamyl-tRNA synthetase K01885 EARS; glutamyl-tRNA synthetase [EC:6.1.1.17]
D PBPRA1034 S0624; putative glutaminyl-tRNA synthetase K01886 QARS; glutaminyl-tRNA synthetase [EC:6.1.1.18]
D PBPRA1064 putative arginyl-tRNA synthetase K01887 RARS; arginyl-tRNA synthetase [EC:6.1.1.19]
D PBPRA2156 Putative phenylalanyl-tRNA synthetase, alpha chain K01889 FARSA; phenylalanyl-tRNA synthetase alpha chain [EC:6.1.1.20]
D PBPRA2155 Putative phenylalanyl-tRNA synthetase, beta chain K01890 FARSB; phenylalanyl-tRNA synthetase beta chain [EC:6.1.1.20]
D PBPRA0764 putative histidyl-tRNA synthetase K01892 HARS; histidyl-tRNA synthetase [EC:6.1.1.21]
D PBPRA2335 ASNS; putative asparaginyl-tRNA synthetase K01893 NARS; asparaginyl-tRNA synthetase [EC:6.1.1.22]
D PBPRA3172 STY0210; putative glutamyl-tRNA synthetase-relatedprotein K01894 gluQ; glutamyl-Q tRNA(Asp) synthetase [EC:6.1.1.-]
C Trans-editing factors
D PBPRA3488 putative D-tyrosyl-tRNA(Tyr) deacylase K07560 dtd; D-aminoacyl-tRNA deacylase [EC:3.1.1.96]
D PBPRA3101 SCO2058; conserved hypothetical protein K03976 ybaK; Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase [EC:3.1.1.-]
D PBPRA2835 STY0541; hypothetical protein ybaK K03976 ybaK; Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase [EC:3.1.1.-]
D PBPRA3128 BA5204; hypothetical DNA-binding protein K19055 prdX; Ala-tRNA(Pro) deacylase [EC:3.1.1.-]
D PBPRA1364 RS00184; hypothetical protein K19055 prdX; Ala-tRNA(Pro) deacylase [EC:3.1.1.-]
D PBPRB1493 R00461; hypothetical protein K19055 prdX; Ala-tRNA(Pro) deacylase [EC:3.1.1.-]
B tRNA modification factors
C Deaminases
D PBPRA0786 STM2568; Putative cytosine/adenosine deaminase K11991 tadA; tRNA(adenine34) deaminase [EC:3.5.4.33]
C Dihydorouridine synthases
D PBPRA3325 VV2972; putative NifR3/Smm1 family protein K05539 dusA; tRNA-dihydrouridine synthase A [EC:1.-.-.-]
D PBPRA3410 VV3138; putative Dihydrouridine synthase K05540 dusB; tRNA-dihydrouridine synthase B [EC:1.-.-.-]
D PBPRA2613 VV2126; putative regulator protein K05541 dusC; tRNA-dihydrouridine synthase C [EC:1.-.-.-]
C Methylthiotransferases
D PBPRA2878 STY0716; putative 2-methylthioadenine synthetase K06168 miaB; tRNA-2-methylthio-N6-dimethylallyladenosine synthase [EC:2.8.4.3]
C Methyltransferases
D PBPRA1110 S2001; Conserved hypothetical protein K15256 cmoA; tRNA (cmo5U34)-methyltransferase [EC:2.1.1.-]
D PBPRA1109 YECP; Conserved hypothetical protein K15257 cmoB; tRNA (mo5U34)-methyltransferase [EC:2.1.1.-]
D PBPRA2379 SMTA; putative SmtA protein K06219 smtA; tRNA 5-carboxymethoxyuridine methyltransferase [EC:2.1.1.-]
D PBPRA3465 PLU4736; putative tRNA (uracil-5-)-methyltransferase K00557 trmA; tRNA (uracil-5-)-methyltransferase [EC:2.1.1.35]
D PBPRA3152 VV2883; putative S-adenosylmethionine-dependent methyltransferase K03439 trmB; tRNA (guanine-N7-)-methyltransferase [EC:2.1.1.33]
D PBPRA3039 TRMD; putative tRNA (guanine-N1)-methyltransferase K00554 trmD; tRNA (guanine37-N1)-methyltransferase [EC:2.1.1.228]
D PBPRA0002 S4013; Putative thiophene and furan oxidation protein ThdF K03650 mnmE; tRNA modification GTPase [EC:3.6.-.-]
D PBPRA0188 Y0104; putative tRNA methyltransferase K00556 trmH; tRNA (guanosine-2'-O-)-methyltransferase [EC:2.1.1.34]
D PBPRA0748 Y1332; putative RNA methyltransferase, TrmH family K15396 trmJ; tRNA (cytidine32/uridine32-2'-O)-methyltransferase [EC:2.1.1.200]
D PBPRA0229 SF3645; putative rRNA methylase, SpoU family K03216 trmL; tRNA (cytidine/uridine-2'-O-)-methyltransferase [EC:2.1.1.207]
D PBPRA2943 ECS0197; conserved hypothetical protein K22900 TRMO; tRNA (adenine37-N6)-methyltransferase [EC:2.1.1.-]
D PBPRA0563 SO0948; hypothetical O-methyltransferase K15460 yfiC; tRNA1Val (adenine37-N6)-methyltransferase [EC:2.1.1.223]
D PBPRA1526 VVA0268; conserved hypothetical protein K16317 trmY; tRNA (pseudouridine54-N1)-methyltransferase [EC:2.1.1.257]
C Psudouridine synthases
D PBPRA2653 Putative tRNA pseudouridine synthase A K06173 truA; tRNA pseudouridine38-40 synthase [EC:5.4.99.12]
D PBPRA0614 EBIG1690; putative tRNA pseudouridine 5S synthase K03177 truB; tRNA pseudouridine55 synthase [EC:5.4.99.25]
D PBPRA2973 VV2561; putative pseudouridine synthase Rlu family protein K06175 truC; tRNA pseudouridine65 synthase [EC:5.4.99.26]
D PBPRA3075 hypothetical hydrogenase subunit K06176 truD; tRNA pseudouridine13 synthase [EC:5.4.99.27]
D PBPRA0408 RLUA; putative ribosomal large subunit pseudouridine synthase A K06177 rluA; tRNA pseudouridine32 synthase / 23S rRNA pseudouridine746 synthase [EC:5.4.99.28 5.4.99.29]
D PBPRA2511 NMB2018; putative pseudouridine synthase Rlu family protein K06177 rluA; tRNA pseudouridine32 synthase / 23S rRNA pseudouridine746 synthase [EC:5.4.99.28 5.4.99.29]
C Thiolation factors
D PBPRA1147 YCFB; putative (5-methylaminomethyl-2-thiouridylate)-methyltransferase K00566 mnmA; tRNA-uridine 2-sulfurtransferase [EC:2.8.1.13]
D PBPRA1841 ECS1928; Putative ATPase K14058 ttcA; tRNA 2-thiocytidine biosynthesis protein TtcA
D PBPRA0060 SIRA; conserved hypothetical protein K04085 tusA; tRNA 2-thiouridine synthesizing protein A [EC:2.8.1.-]
D PBPRA0312 SF3361; hypothetical protein K07237 tusB; tRNA 2-thiouridine synthesizing protein B
D PBPRA0311 SF3362; conserved hypothetical protein K07236 tusC; tRNA 2-thiouridine synthesizing protein C
D PBPRA0310 SF3363; conserved hypothetical protein K07235 tusD; tRNA 2-thiouridine synthesizing protein D [EC:2.8.1.-]
D PBPRA2191 STM1084; putative sulfite reductase K11179 tusE; tRNA 2-thiouridine synthesizing protein E [EC:2.8.1.-]
D PBPRA0752 STM2541; Putative hesB family protein K13628 iscA; iron-sulfur cluster assembly protein
D PBPRA0750 PLU3283; Putative aminotransferase NifS, class V K04487 iscS; cysteine desulfurase [EC:2.8.1.7]
D PBPRA3100 STY3204; hypothetical protein K06980 ygfZ; tRNA-modifying protein YgfZ
C KEOPS/EKC complex
C Other tRNA modification factors
D PBPRA3615 S3947; Putative glucose inhibited division protein A K03495 gidA; tRNA uridine 5-carboxymethylaminomethyl modification enzyme
D PBPRA3351 VV3072; putative tRNA delta(2)-isopentenylpyrophosphate transferase K00791 miaA; tRNA dimethylallyltransferase [EC:2.5.1.75]
D PBPRA2472 STY4809; putative tRNA-(MS[2]IO[6]A)-hydroxylase K06169 miaE; tRNA 2-(methylsulfanyl)-N6-isopentenyladenosine37 hydroxylase [EC:1.14.99.69]
D PBPRA2659 hypothetical peptidase K15461 mnmC; tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein [EC:2.1.1.61 1.5.-.-]
D PBPRA0742 QUEA; putative S-adenosylmethionine:tRNAribosyltransferase-isomerase K07568 queA; S-adenosylmethionine:tRNA ribosyltransferase-isomerase [EC:2.4.99.17]
D PBPRA0968 ECS0498; putative exsB protein K06920 queC; 7-cyano-7-deazaguanine synthase [EC:6.3.4.20]
D PBPRA2333 STY3080; putative organic radical activating enzymes K10026 queE; 7-carboxy-7-deazaguanine synthase [EC:4.3.99.3]
D PBPRA2982 T2876; conserved hypothetical protein K06879 queF; 7-cyano-7-deazaguanine reductase [EC:1.7.1.13]
D PBPRA3356 YJES; putative iron-sulfur cluster-binding protein K18979 queG; epoxyqueuosine reductase [EC:1.17.99.6]
D PBPRA2592 PLU2551; Putative selenophosphate synthase K01008 selD; selenide, water dikinase [EC:2.7.9.3]
D PBPRB1545 EF2567; Hypothetical selenophosphate synthase K01008 selD; selenide, water dikinase [EC:2.7.9.3]
D PBPRA2591 Z0657; hypothetical ATPase K06917 selU; tRNA 2-selenouridine synthase [EC:2.9.1.3]
D PBPRA0743 TGT; Putative queuine tRNA-ribosyltransferase K00773 tgt; queuine tRNA-ribosyltransferase [EC:2.4.2.29]
D PBPRA0810 Putative thiamin biosynthesis protein, ThiI K03151 thiI; tRNA uracil 4-sulfurtransferase [EC:2.8.1.4]
D PBPRA2951 SF0178; hypothetical cell cycle protein MesJ K04075 tilS; tRNA(Ile)-lysidine synthase [EC:6.3.4.19]
D PBPRB2023 conserved hypothetical protein K06957 tmcA; tRNA(Met) cytidine acetyltransferase [EC:2.3.1.193]
D PBPRA1071 YEAZ; Conserved hypothetical protein K14742 tsaB; tRNA threonylcarbamoyladenosine biosynthesis protein TsaB
D PBPRA3573 YRDC; Putative Sua5/YciO/YrdC family protein K07566 tsaC; L-threonylcarbamoyladenylate synthase [EC:2.7.7.87]
D PBPRA0434 SF3105; putative O-sialoglycoprotein endopeptidase K25706 tsaD; tRNA N6-adenosine threonylcarbamoyltransferase [EC:2.3.1.234]
D PBPRA3354 Y0626; putative nucleotide-binding protein K06925 tsaE; tRNA threonylcarbamoyladenosine biosynthesis protein TsaE
D PBPRA2993 Z4129; putative HesA/MoeB/ThiF family protein K22132 tcdA; tRNA threonylcarbamoyladenosine dehydratase
B tRNA degradation factors
C PBPRA3341 STM4368; putative Exoribonuclease R K12573 rnr; ribonuclease R [EC:3.1.13.1]
C PBPRA0616 C3920; putative polyribonucleotide nucleotidyltransferase K00962 pnp; polyribonucleotide nucleotidyltransferase [EC:2.7.7.8]
!
#
#[ BRITE | KEGG2 | KEGG ]
#Last updated: February 29, 2024