KAAS (KEGG Automatic Annotation Server) provides functional annotation of genes by BLAST comparisons against the manually curated KEGG GENES database. The result contains KO (KEGG Orthology) assignments and automatically generated KEGG pathways.
Complete or Draft Genome
KAAS works best when a complete set of genes in a genome is known. Prepare query amino acid sequences and use the BBH (bi-directional best hit) method to assign orthologs.
KAAS can also be used for a limited number of genes. Prepare query amino acid sequences and use the SBH (single-directional best hit) method to assign orthologs.
When ESTs are comprehensive enough, a set of consensus contigs can be generated by the EGassembler server and used as a gene set for KAAS with the BBH method. Otherwise, use ESTs as they are with the SBH method.
Example of Results
KEGG pathway mapping