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Entry
EC 1.14.19.60               Enzyme                                 

Name
7-chloro-L-tryptophan 6-halogenase;
ktzR (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
BRITE hierarchy
Sysname
7-chloro-L-tryptophan:FADH2 oxidoreductase (6-halogenating)
Reaction(IUBMB)
7-chloro-L-tryptophan + FADH2 + chloride + O2 + H+ = 6,7-dichloro-L-tryptophan + FAD + 2 H2O [RN:R12034]
Reaction(KEGG)
Substrate
7-chloro-L-tryptophan [CPD:C19687];
FADH2 [CPD:C01352];
chloride [CPD:C00698];
O2 [CPD:C00007];
H+ [CPD:C00080]
Product
6,7-dichloro-L-tryptophan [CPD:C21868];
FAD [CPD:C00016];
H2O [CPD:C00001]
Comment
An FAD-dependent halogenase. The enzyme, characterized from the bacterium Kutzneria sp. 744, works in tandem with EC 1.14.19.9, tryptophan 7-halogenase, (ktzQ) to generate 6,7-dichloro-L-tryptophan, which is incorporated as a pyrroloindoline in the kutznerides family of natural products. It has a 120-fold preference for 7-chloro-L-tryptophan over L-tryptophan as substrate.
History
EC 1.14.19.60 created 2018
Orthology
K22695  7-chloro-L-tryptophan 6-halogenase
Reference
1  [PMID:18828589]
  Authors
Heemstra JR Jr, Walsh CT
  Title
Tandem action of the O2- and FADH2-dependent halogenases KtzQ and KtzR produce 6,7-dichlorotryptophan for kutzneride assembly.
  Journal
J Am Chem Soc 130:14024-5 (2008)
DOI:10.1021/ja806467a
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.60
IUBMB Enzyme Nomenclature: 1.14.19.60
ExPASy - ENZYME nomenclature database: 1.14.19.60
BRENDA, the Enzyme Database: 1.14.19.60

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