KEGG ENZYME: 1.8.1.5

ENZYME: 1.8.1.5

Entry

EC 1.8.1.5                  Enzyme

Name

2-oxopropyl-CoM reductase (carboxylating);
NADPH:2-(2-ketopropylthio)ethanesulfonate oxidoreductase/carboxylase;
NADPH:2-ketopropyl-coenzyme M oxidoreductase/carboxylase;
2-mercaptoethanesulfonate,acetoacetate:NADP+ oxidoreductase (decarboxylating)

Class

Oxidoreductases;
Acting on a sulfur group of donors;
With NAD+ or NADP+ as acceptor

Sysname

2-sulfanylethane-1-sulfonate,acetoacetate:NADP+ oxidoreductase (decarboxylating)

Reaction(IUBMB)

CoM + acetoacetate + NADP+ = 2-oxopropyl-CoM + CO2 + NADPH [RN:R05713]

Reaction(KEGG)

R05713

Substrate

CoM [CPD:C03576];
acetoacetate [CPD:C00164];
NADP+ [CPD:C00006]

Product

2-oxopropyl-CoM [CPD:C11497];
CO2 [CPD:C00011];
NADPH [CPD:C00005]

Comment

Also acts on thioethers longer in chain length on the oxo side, e.g. 2-oxobutyl-CoM, but this portion must be attached to CoM (2-sulfanylethane-1-sulfonate); no CoM analogs will substitute. This enzyme forms component II of a four-component enzyme system {comprising EC 4.4.1.23 (2-hydroxypropyl-CoM lyase; component I), EC 1.8.1.5 [2-oxopropyl-CoM reductase (carboxylating); component II], EC 1.1.1.268 [2-(R)-hydroxypropyl-CoM dehydrogenase; component III] and EC 1.1.1.269 [2-(S)-hydroxypropyl-CoM dehydrogenase; component IV]} that is involved in epoxyalkane carboxylation in Xanthobacter sp. strain Py2.

History

EC 1.8.1.5 created 2001

Orthology

K22364

2-oxopropyl-CoM reductase (carboxylating)

Genes

XAU:	Xaut_4867 Xaut_5048

MEDK:

QEV83_11410

MIWA:

SS37A_25570

HADH:

FRZ61_48030(gor)

BTS:

Btus_2703

MSA:	Mycsm_01895

MCB:	Mycch_5445 Mycch_5461 Mycch_5985

MRH:	MycrhN_2978 MycrhN_3082

NCA:

Noca_4827

ANW:	QP939_20415

Reference

1 [PMID:10411892]

Authors

Allen JR, Clark DD, Krum JG, Ensign SA.

Title

A role for coenzyme M (2-mercaptoethanesulfonic acid) in a bacterial pathway of aliphatic epoxide carboxylation.

Journal

Proc Natl Acad Sci U S A 96:8432-7 (1999)
DOI:10.1073/pnas.96.15.8432

Reference

2 [PMID:10684609]

Authors

Clark DD, Allen JR, Ensign SA.

Title

Characterization of five catalytic activities associated with the NADPH:2-ketopropyl-coenzyme M [2-(2-ketopropylthio)ethanesulfonate] oxidoreductase/carboxylase of the Xanthobacter strain Py2 epoxide carboxylase system.

Journal

Biochemistry 39:1294-304 (2000)
DOI:10.1021/bi992282p

Other DBs

ExplorEnz - The Enzyme Database:

1.8.1.5

IUBMB Enzyme Nomenclature:

1.8.1.5

ExPASy - ENZYME nomenclature database:

1.8.1.5

UM-BBD (Biocatalysis/Biodegradation Database):

1.8.1.5

BRENDA, the Enzyme Database:

1.8.1.5

CAS:	244301-63-1

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