Entry Name colneleate synthase;
Class Lyases;BRITE hierarchy
Sysname (9S,10E,12Z)-9-hydroperoxyoctadeca-10,12-dienoate hydro-lyase
Reaction(IUBMB) (9S,10E,12Z)-9-hydroperoxyoctadeca-10,12-dienoate = (8E)-9-[(1E,3Z)-nona-1,3-dien-1-yloxy]non-8-enoate + H2O [RN:
R09699 ]
Reaction(KEGG) Substrate (9S,10E,12Z)-9-hydroperoxyoctadeca-10,12-dienoate
Product (8E)-9-[(1E,3Z)-nona-1,3-dien-1-yloxy]non-8-enoate [CPD:
C19827 ];
H2O [CPD:
C00001 ]
Comment A heme-thiolate protein (P-450) [2]. It catalyses the selective removal of pro-R hydrogen at C-8 in the biosynthesis of colneleic acid [4]. It forms also (8E)-9-[(1E,3Z,6Z)-nona-1,3,6-trien-1-yloxy]non-8-enoic acid (i.e. colnelenate) from (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate. The corresponding 13-hydroperoxides are poor substrates [1,3]. The divinyl ethers colneleate and colnelenate have antimicrobial activity.
History EC 4.2.1.121 created 2011, modified 2014
Orthology K17874 9-divinyl ether synthase
Genes NTA : 107799697(CYP74D3) 107808154
Taxonomy Reference Authors Stumpe M, Kandzia R, Gobel C, Rosahl S, Feussner I
Title A pathogen-inducible divinyl ether synthase (CYP74D) from elicitor-treated potato suspension cells.
Journal Sequence Reference Authors Itoh A, Howe GA
Title Molecular cloning of a divinyl ether synthase. Identification as a CYP74 cytochrome P-450.
Journal Sequence Reference Authors Fammartino A, Cardinale F, Gobel C, Mene-Saffrane L, Fournier J, Feussner I, Esquerre-Tugaye MT
Title Characterization of a divinyl ether biosynthetic pathway specifically associated with pathogenesis in tobacco.
Journal Reference Authors Hamberg M.
Title Hidden stereospecificity in the biosynthesis of divinyl ether fatty acids.
Journal Sequence Other DBs ExPASy - ENZYME nomenclature database: 4.2.1.121
