KEGG   ENZYME: 4.2.3.87Help
Entry
EC 4.2.3.87                 Enzyme                                 

Name
alpha-guaiene synthase;
PatTps177 (gene name)
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
BRITE hierarchy
Sysname
(2Z,6E)-farnesyl-diphosphate diphosphate-lyase (cyclizing, alpha-guaiene-forming)
Reaction(IUBMB)
(2E,6E)-farnesyl diphosphate = alpha-guaiene + diphosphate [RN:R09887]
Reaction(KEGG)
Substrate
(2E,6E)-farnesyl diphosphate [CPD:C00448]
Product
alpha-guaiene [CPD:C20161];
diphosphate [CPD:C00013]
Comment
Requires Mg2+. The enzyme from Pogostemon cablin gives 13% alpha-guaiene as well as 37% (-)-patchoulol (see EC 4.2.3.70), 13% delta-guaiene (see EC 4.2.3.93), and traces of at least ten other sesquiterpenoids [1]. In Aquilaria crassna three clones of the enzyme gave about 80% delta-guaiene and 20% alpha-guaiene, with traces of alpha-humulene. A fourth clone gave 54% delta-guaiene and 45% alpha-guaiene [2].
History
EC 4.2.3.87 created 2011
Orthology
K15807  patchoulol/alpha-guaiene/delta-guaiene synthase
K22036  alpha/delta-guaiene synthase
Reference
1  [PMID:16970904]
  Authors
Deguerry F, Pastore L, Wu S, Clark A, Chappell J, Schalk M
  Title
The diverse sesquiterpene profile of patchouli, Pogostemon cablin, is correlated  with a limited number of sesquiterpene synthases.
  Journal
Arch Biochem Biophys 454:123-36 (2006)
DOI:10.1016/j.abb.2006.08.006
  Sequence
Reference
2  [PMID:20959422]
  Authors
Kumeta Y, Ito M
  Title
Characterization of delta-guaiene synthases from cultured cells of Aquilaria, responsible for the formation of the sesquiterpenes in agarwood.
  Journal
Plant Physiol 154:1998-2007 (2010)
DOI:10.1104/pp.110.161828
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.87
IUBMB Enzyme Nomenclature: 4.2.3.87
ExPASy - ENZYME nomenclature database: 4.2.3.87
BRENDA, the Enzyme Database: 4.2.3.87

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