KEGG   PATHWAY: atd00510Help
Entry
atd00510                    Pathway                                

Name
N-Glycan biosynthesis - Aethina tumida (small hive beetle)
Description
N-glycans or asparagine-linked glycans are major constituents of glycoproteins in eukaryotes. N-glycans are covalently attached to asparagine with the consensus sequence of Asn-X-Ser/Thr by an N-glycosidic bond, GlcNAc b1- Asn. Biosynthesis of N-glycans begins on the cytoplasmic face of the ER membrane with the transferase reaction of UDP-GlcNAc and the lipid-like precursor P-Dol (dolichol phosphate) to generate GlcNAc a1- PP-Dol. After sequential addition of monosaccharides by ALG glycosyltransferases [MD:M00055], the N-glycan precursor is attached by the OST (oligosaccharyltransferase) complex to the polypeptide chain that is being synthesized and translocated through the ER membrane. The protein-bound N-glycan precursor is subsequently trimmed, extended, and modified in the ER and Golgi by a complex series of reactions catalyzed by membrane-bound glycosidases and glycosyltransferases. N-glycans thus synthesized are classified into three types: high-mannose type, complex type, and hybrid type. Defects in N-glycan biosynthesis lead to a variety of human diseases known as congenital disorders of glycosylation [DS:H00118 H00119].
Class
Metabolism; Glycan biosynthesis and metabolism
BRITE hierarchy
Pathway map
atd00510  N-Glycan biosynthesis
atd00510

Ortholog table
Module
atd_M00055  N-glycan precursor biosynthesis [PATH:atd00510]
atd_M00072  N-glycosylation by oligosaccharyltransferase [PATH:atd00510]
atd_M00073  N-glycan precursor trimming [PATH:atd00510]
Other DBs
GO: 0006487
Organism
Aethina tumida (small hive beetle) [GN:atd]
Gene
109597739  polyprenol reductase isoform X1 [KO:K12345] [EC:1.3.1.22 1.3.1.94]
109605975  LOW QUALITY PROTEIN: dolichol kinase [KO:K00902] [EC:2.7.1.108]
109597742  UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase [KO:K01001] [EC:2.7.8.15]
109605178  dolichyl-phosphate beta-glucosyltransferase [KO:K00729] [EC:2.4.1.117]
109593961  UDP-N-acetylglucosamine transferase subunit ALG13 homolog [KO:K07432] [EC:2.4.1.141]
109596272  LOW QUALITY PROTEIN: UDP-N-acetylglucosamine transferase subunit ALG14 homolog [KO:K07441] [EC:2.4.1.141]
109595128  probable dolichol-phosphate mannosyltransferase [KO:K00721] [EC:2.4.1.83]
109604175  probable dolichol-phosphate mannosyltransferase subunit 3 [KO:K09659]
109606374  LOW QUALITY PROTEIN: chitobiosyldiphosphodolichol beta-mannosyltransferase [KO:K03842] [EC:2.4.1.142]
109601758  alpha-1,3/1,6-mannosyltransferase ALG2 [KO:K03843] [EC:2.4.1.132 2.4.1.257]
109607356  alpha-1,3/1,6-mannosyltransferase ALG2-like [KO:K03843] [EC:2.4.1.132 2.4.1.257]
109606232  LOW QUALITY PROTEIN: GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase [KO:K03844] [EC:2.4.1.131]
109600158  lethal(2)neighbour of tid protein [KO:K03845] [EC:2.4.1.258]
109604351  LOW QUALITY PROTEIN: alpha-1,2-mannosyltransferase ALG9 [KO:K03846] [EC:2.4.1.259 2.4.1.261]
109601432  LOW QUALITY PROTEIN: probable Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase [KO:K03847] [EC:2.4.1.260]
109602995  dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase [KO:K03848] [EC:2.4.1.267]
109597402  probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase [KO:K03849] [EC:2.4.1.265]
109598081  LOW QUALITY PROTEIN: putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase [KO:K03850] [EC:2.4.1.256]
109594213  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B [KO:K07151] [EC:2.4.99.18]
109603298  LOW QUALITY PROTEIN: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A [KO:K07151] [EC:2.4.99.18]
109596153  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 [KO:K12666]
109596114  LOW QUALITY PROTEIN: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2-like [KO:K12667]
109608069  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2-like [KO:K12667]
109594828  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 [KO:K12668]
109605305  tumor suppressor candidate 3-like [KO:K12669]
109607191  LOW QUALITY PROTEIN: tumor suppressor candidate 3-like [KO:K12669]
109595969  dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit [KO:K12670]
109600101  LOW QUALITY PROTEIN: dolichyldiphosphatase 1-like [KO:K07252] [EC:3.6.1.43]
109597104  mannosyl-oligosaccharide glucosidase GCS1 [KO:K01228] [EC:3.2.1.106]
109603135  LOW QUALITY PROTEIN: neutral alpha-glucosidase C-like [KO:K05546] [EC:3.2.1.207]
109603144  LOW QUALITY PROTEIN: neutral alpha-glucosidase AB-like [KO:K05546] [EC:3.2.1.207]
109609021  LOW QUALITY PROTEIN: neutral alpha-glucosidase AB-like [KO:K05546] [EC:3.2.1.207]
109598739  endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase [KO:K23741] [EC:3.2.1.209]
109606581  LOW QUALITY PROTEIN: mannosyl-oligosaccharide alpha-1,2-mannosidase IA-like [KO:K01230] [EC:3.2.1.113]
109599179  mannosyl-oligosaccharide 1,2-alpha-mannosidase IA-like [KO:K01230] [EC:3.2.1.113]
109597350  alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [KO:K00726] [EC:2.4.1.101]
109605290  alpha-mannosidase 2 [KO:K01231] [EC:3.2.1.114]
109608308  alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase isoform X1 [KO:K00736] [EC:2.4.1.143]
109601071  alpha-(1,6)-fucosyltransferase [KO:K00717] [EC:2.4.1.68]
109597740  beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase [KO:K00737] [EC:2.4.1.144]
109595235  alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B isoform X1 [KO:K00738] [EC:2.4.1.145]
Compound
C00096  GDP-mannose
C00110  Dolichyl phosphate
C00381  Dolichol
C00621  Dolichyl diphosphate
C01246  Dolichyl beta-D-glucosyl phosphate
C03021  Protein asparagine
C03862  Dolichyl phosphate D-mannose
C06081  Polyprenol
G00001  N-Acetyl-D-glucosaminyldiphosphodolichol
G00002  N,N'-Chitobiosyldiphosphodolichol
G00003   
G00004   
G00005   
G00006   
G00007   
G00008   
G00009   
G00010   
G00011   
G00012   
G00013   
G00014   
G00015   
G00016   
G00017   
G00018  DS 3
G00019   
G00020   
G00021   
G00022   
G00171   
G10526   
G10595   
G10596   
G10597   
G10598   
G10599   
G10694   
Reference
PMID:9111134
  Authors
Schutzbach JS.
  Title
The role of the lipid matrix in the biosynthesis of dolichyl-linked oligosaccharides.
  Journal
Glycoconj J 14:175-82 (1997)
DOI:10.1023/A:1018533620002
Reference
  Authors
Oriol R, Martinez-Duncker I, Chantret I, Mollicone R, Codogno P.
  Title
Common origin and evolution of glycosyltransferases using Dol-P-monosaccharides as donor substrate.
  Journal
Mol Biol Evol 19:1451-63 (2002)
DOI:10.1093/oxfordjournals.molbev.a004208
Reference
  Authors
Thiel C, Schwarz M, Peng J, Grzmil M, Hasilik M, Braulke T, Kohlschutter A, von Figura K, Lehle L, Korner C.
  Title
A new type of congenital disorders of glycosylation (CDG-Ii) provides new insights into the early steps of dolichol-linked oligosaccharide biosynthesis.
  Journal
J Biol Chem 278:22498-505 (2003)
DOI:10.1074/jbc.M302850200
Reference
  Authors
Cipollo JF, Trimble RB, Chi JH, Yan Q, Dean N.
  Title
The yeast ALG11 gene specifies addition of the terminal alpha 1,2-Man to the Man5GlcNAc2-PP-dolichol N-glycosylation intermediate formed on the cytosolic side of the endoplasmic reticulum.
  Journal
J Biol Chem 276:21828-40 (2001)
DOI:10.1074/jbc.M010896200
Reference
  Authors
Frank CG, Aebi M.
  Title
ALG9 mannosyltransferase is involved in two different steps of lipid-linked oligosaccharide biosynthesis.
  Journal
Glycobiology 15:1156-63 (2005)
DOI:10.1093/glycob/cwj002
Reference
  Authors
O'Reilly MK, Zhang G, Imperiali B.
  Title
In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis.
  Journal
Biochemistry 45:9593-603 (2006)
DOI:10.1021/bi060878o
Related
pathway
atd00051  Fructose and mannose metabolism
atd00511  Other glycan degradation
atd00513  Various types of N-glycan biosynthesis
atd00515  Mannose type O-glycan biosynthesis
atd00520  Amino sugar and nucleotide sugar metabolism
atd00563  Glycosylphosphatidylinositol (GPI)-anchor biosynthesis
atd00900  Terpenoid backbone biosynthesis
KO pathway
ko00510   

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