KEGG   PATHWAY: dme00510
Entry
dme00510                    Pathway                                

Name
N-Glycan biosynthesis - Drosophila melanogaster (fruit fly)
Description
N-glycans or asparagine-linked glycans are major constituents of glycoproteins in eukaryotes. N-glycans are covalently attached to asparagine with the consensus sequence of Asn-X-Ser/Thr by an N-glycosidic bond, GlcNAc b1- Asn. Biosynthesis of N-glycans begins on the cytoplasmic face of the ER membrane with the transferase reaction of UDP-GlcNAc and the lipid-like precursor P-Dol (dolichol phosphate) to generate GlcNAc a1- PP-Dol. After sequential addition of monosaccharides by ALG glycosyltransferases [MD:M00055], the N-glycan precursor is attached by the OST (oligosaccharyltransferase) complex to the polypeptide chain that is being synthesized and translocated through the ER membrane. The protein-bound N-glycan precursor is subsequently trimmed, extended, and modified in the ER and Golgi by a complex series of reactions catalyzed by membrane-bound glycosidases and glycosyltransferases. N-glycans thus synthesized are classified into three types: high-mannose type, complex type, and hybrid type. Defects in N-glycan biosynthesis lead to a variety of human diseases known as congenital disorders of glycosylation [DS:H00118 H00119].
Class
Metabolism; Glycan biosynthesis and metabolism
Pathway map
dme00510  N-Glycan biosynthesis
dme00510

Module
dme_M00055  N-glycan precursor biosynthesis [PATH:dme00510]
dme_M00072  N-glycosylation by oligosaccharyltransferase [PATH:dme00510]
dme_M00073  N-glycan precursor trimming [PATH:dme00510]
Other DBs
GO: 0006487
Organism
Drosophila melanogaster (fruit fly) [GN:dme]
Gene
Dmel_CG7840  CG7840; uncharacterized protein [KO:K12345] [EC:1.3.1.22 1.3.1.94]
Dmel_CG8311  CG8311; uncharacterized protein [KO:K00902] [EC:2.7.1.108]
Dmel_CG5287  CG5287; uncharacterized protein [KO:K01001] [EC:2.7.8.15]
Dmel_CG7870  wol; wollknaeuel [KO:K00729] [EC:2.4.1.117]
Dmel_CG14512  CG14512; uncharacterized protein [KO:K07432] [EC:2.4.1.141]
Dmel_CG6308  Alg14; ALG14, UDP-N-acetylglucosaminyltransferase subunit [KO:K07441] [EC:2.4.1.141]
Dmel_CG10166  CG10166; uncharacterized protein [KO:K00721] [EC:2.4.1.83]
Dmel_CG42456  CG42456; uncharacterized protein [KO:K09658]
Dmel_CG33977  CG33977; uncharacterized protein [KO:K09659]
Dmel_CG18012  Alg1; ALG1, chitobiosyldiphosphodolichol beta-mannosyltransferase [KO:K03842] [EC:2.4.1.142]
Dmel_CG1291  Alg2; ALG2, alpha-1,3/1,6-mannosyltransferase [KO:K03843] [EC:2.4.1.132 2.4.1.257]
Dmel_CG11306  Alg11; ALG11, alpha-1,2-mannosyltransferase, isoform B [KO:K03844] [EC:2.4.1.131]
Dmel_CG4084  Alg3; ALG3, alpha-1,3- mannosyltransferase [KO:K03845] [EC:2.4.1.258]
Dmel_CG11851  Alg9; ALG9, alpha-1,2-mannosyltransferase [KO:K03846] [EC:2.4.1.259 2.4.1.261]
Dmel_CG8412  CG8412; uncharacterized protein [KO:K03847] [EC:2.4.1.260]
Dmel_CG5091  gny; garnysstan, isoform B [KO:K03848] [EC:2.4.1.267]
Dmel_CG4542  xit; xiantuan [KO:K03849] [EC:2.4.1.265]
Dmel_CG32076  Alg10; ALG10 alpha-1,2-glucosyltransferase [KO:K03850] [EC:2.4.1.256]
Dmel_CG7748  Stt3B; catalytic subunit 3B of the oligosaccharyltransferase complex [KO:K07151] [EC:2.4.99.18]
Dmel_CG1518  Stt3A; catalytic subunit 3A of the oligosaccharyltransferase complex, isoform C [KO:K07151] [EC:2.4.99.18]
Dmel_CG33303  CG33303; uncharacterized protein, isoform B [KO:K12666]
Dmel_CG6370  OstDelta; oligosaccharide transferase delta subunit, isoform B [KO:K12667]
Dmel_CG13393  Dad1; defender against apoptotic cell death 1 [KO:K12668]
Dmel_CG7830  Ostgamma; oligosaccharide transferase gamma subunit [KO:K12669]
Dmel_CG9022  Ost48; oligosaccharyltransferase 48kD subunit [KO:K12670]
Dmel_CG1597  GCS1; glucosidase 1, isoform C [KO:K01228] [EC:3.2.1.106]
Dmel_CG14476  GCS2alpha; glucosidase 2 alpha subunit, isoform B [KO:K05546] [EC:3.2.1.207]
Dmel_CG11874  alpha-Man-Ib; alpha-Mannosidase class I b [KO:K23741] [EC:3.2.1.209]
Dmel_CG31202  alpha-Man-Ic; alpha-Mannosidase class I c [KO:K01230] [EC:3.2.1.113]
Dmel_CG42275  alpha-Man-Ia; alpha-Mannosidase class I a, isoform J [KO:K01230] [EC:3.2.1.113]
Dmel_CG13431  Mgat1; mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase [KO:K00726] [EC:2.4.1.101]
Dmel_CG18802  alpha-Man-IIa; alpha-Mannosidase class II a, isoform A [KO:K01231] [EC:3.2.1.114]
Dmel_CG7921  Mgat2; mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase, isoform B [KO:K00736] [EC:2.4.1.143]
Dmel_CG2448  FucT6; alpha1,6-fucosyltransferase [KO:K00717] [EC:2.4.1.68]
Dmel_CG4871  SiaT; sialyltransferase, isoform B [KO:K00778] [EC:2.4.99.1]
Dmel_CG31849  CG31849; uncharacterized protein [KO:K00737] [EC:2.4.1.144]
Dmel_CG9384  CG9384; uncharacterized protein, isoform B [KO:K00738] [EC:2.4.1.145]
Dmel_CG17173  CG17173; uncharacterized protein [KO:K00738] [EC:2.4.1.145]
Compound
C00096  GDP-mannose
C00110  Dolichyl phosphate
C00381  Dolichol
C00621  Dolichyl diphosphate
C01246  Dolichyl beta-D-glucosyl phosphate
C03021  Protein asparagine
C03862  Dolichyl phosphate D-mannose
C06081  Polyprenol
G00001  N-Acetyl-D-glucosaminyldiphosphodolichol
G00002  N,N'-Chitobiosyldiphosphodolichol
G00003   
G00004   
G00005   
G00006   
G00007   
G00008   
G00009   
G00010   
G00011   
G00012   
G00013   
G00014   
G00015   
G00016   
G00017   
G00018  DS 3
G00019   
G00020   
G00021   
G00022   
G00171   
G10526   
G10595   
G10596   
G10597   
G10598   
G10599   
G10694   
Reference
PMID:9111134
  Authors
Schutzbach JS.
  Title
The role of the lipid matrix in the biosynthesis of dolichyl-linked oligosaccharides.
  Journal
Glycoconj J 14:175-82 (1997)
DOI:10.1023/A:1018533620002
Reference
  Authors
Oriol R, Martinez-Duncker I, Chantret I, Mollicone R, Codogno P.
  Title
Common origin and evolution of glycosyltransferases using Dol-P-monosaccharides as donor substrate.
  Journal
Mol Biol Evol 19:1451-63 (2002)
DOI:10.1093/oxfordjournals.molbev.a004208
Reference
  Authors
Thiel C, Schwarz M, Peng J, Grzmil M, Hasilik M, Braulke T, Kohlschutter A, von Figura K, Lehle L, Korner C.
  Title
A new type of congenital disorders of glycosylation (CDG-Ii) provides new insights into the early steps of dolichol-linked oligosaccharide biosynthesis.
  Journal
J Biol Chem 278:22498-505 (2003)
DOI:10.1074/jbc.M302850200
Reference
  Authors
Cipollo JF, Trimble RB, Chi JH, Yan Q, Dean N.
  Title
The yeast ALG11 gene specifies addition of the terminal alpha 1,2-Man to the Man5GlcNAc2-PP-dolichol N-glycosylation intermediate formed on the cytosolic side of the endoplasmic reticulum.
  Journal
J Biol Chem 276:21828-40 (2001)
DOI:10.1074/jbc.M010896200
Reference
  Authors
Frank CG, Aebi M.
  Title
ALG9 mannosyltransferase is involved in two different steps of lipid-linked oligosaccharide biosynthesis.
  Journal
Glycobiology 15:1156-63 (2005)
DOI:10.1093/glycob/cwj002
Reference
  Authors
O'Reilly MK, Zhang G, Imperiali B.
  Title
In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis.
  Journal
Biochemistry 45:9593-603 (2006)
DOI:10.1021/bi060878o
Related
pathway
dme00051  Fructose and mannose metabolism
dme00511  Other glycan degradation
dme00513  Various types of N-glycan biosynthesis
dme00515  Mannose type O-glycan biosynthesis
dme00520  Amino sugar and nucleotide sugar metabolism
dme00563  Glycosylphosphatidylinositol (GPI)-anchor biosynthesis
dme00900  Terpenoid backbone biosynthesis
KO pathway
ko00510   

DBGET integrated database retrieval system