Entry |
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Name |
N-Glycan biosynthesis - Drosophila pseudoobscura pseudoobscura
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Description |
N-glycans or asparagine-linked glycans are major constituents of glycoproteins in eukaryotes. N-glycans are covalently attached to asparagine with the consensus sequence of Asn-X-Ser/Thr by an N-glycosidic bond, GlcNAc b1- Asn. Biosynthesis of N-glycans begins on the cytoplasmic face of the ER membrane with the transferase reaction of UDP-GlcNAc and the lipid-like precursor P-Dol (dolichol phosphate) to generate GlcNAc a1- PP-Dol. After sequential addition of monosaccharides by ALG glycosyltransferases [MD: M00055], the N-glycan precursor is attached by the OST (oligosaccharyltransferase) complex to the polypeptide chain that is being synthesized and translocated through the ER membrane. The protein-bound N-glycan precursor is subsequently trimmed, extended, and modified in the ER and Golgi by a complex series of reactions catalyzed by membrane-bound glycosidases and glycosyltransferases. N-glycans thus synthesized are classified into three types: high-mannose type, complex type, and hybrid type. Defects in N-glycan biosynthesis lead to a variety of human diseases known as congenital disorders of glycosylation [DS: H00118 H00119].
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Class |
Metabolism; Glycan biosynthesis and metabolism
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Pathway map |

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Module |
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Other DBs |
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Organism |
Drosophila pseudoobscura pseudoobscura [GN: dpo]
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Gene |
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Compound |
C01246 | Dolichyl beta-D-glucosyl phosphate |
C03862 | Dolichyl phosphate D-mannose |
G00001 | N-Acetyl-D-glucosaminyldiphosphodolichol |
G00002 | N,N'-Chitobiosyldiphosphodolichol |
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Reference |
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Authors |
Schutzbach JS. |
Title |
The role of the lipid matrix in the biosynthesis of dolichyl-linked oligosaccharides. |
Journal |
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Reference |
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Authors |
Oriol R, Martinez-Duncker I, Chantret I, Mollicone R, Codogno P. |
Title |
Common origin and evolution of glycosyltransferases using Dol-P-monosaccharides as donor substrate. |
Journal |
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Reference |
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Authors |
Thiel C, Schwarz M, Peng J, Grzmil M, Hasilik M, Braulke T, Kohlschutter A, von Figura K, Lehle L, Korner C. |
Title |
A new type of congenital disorders of glycosylation (CDG-Ii) provides new insights into the early steps of dolichol-linked oligosaccharide biosynthesis. |
Journal |
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Reference |
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Authors |
Cipollo JF, Trimble RB, Chi JH, Yan Q, Dean N. |
Title |
The yeast ALG11 gene specifies addition of the terminal alpha 1,2-Man to the Man5GlcNAc2-PP-dolichol N-glycosylation intermediate formed on the cytosolic side of the endoplasmic reticulum. |
Journal |
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Reference |
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Authors |
Frank CG, Aebi M. |
Title |
ALG9 mannosyltransferase is involved in two different steps of lipid-linked oligosaccharide biosynthesis. |
Journal |
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Reference |
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Authors |
O'Reilly MK, Zhang G, Imperiali B. |
Title |
In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis. |
Journal |
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Related pathway |
dpo00051 | Fructose and mannose metabolism |
dpo00513 | Various types of N-glycan biosynthesis |
dpo00515 | Mannose type O-glycan biosynthesis |
dpo00520 | Amino sugar and nucleotide sugar metabolism |
dpo00533 | Glycosaminoglycan biosynthesis - keratan sulfate |
dpo00563 | Glycosylphosphatidylinositol (GPI)-anchor biosynthesis |
dpo00900 | Terpenoid backbone biosynthesis |
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KO pathway |
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