LOCUS NC_001137 576874 bp DNA linear CON 23-JAN-2026
DEFINITION Saccharomyces cerevisiae S288C chromosome V, complete sequence.
ACCESSION NC_001137
VERSION NC_001137.3
DBLINK BioProject: PRJNA128
Assembly: GCF_000146045.2
KEYWORDS RefSeq.
SOURCE Saccharomyces cerevisiae S288C
ORGANISM Saccharomyces cerevisiae S288C
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
Saccharomyces.
REFERENCE 1 (bases 1 to 576874)
AUTHORS Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M.,
Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S.,
Weng,S. and Cherry,J.M.
TITLE New data and collaborations at the Saccharomyces Genome Database:
updated reference genome, alleles, and the Alliance of Genome
Resources
JOURNAL Genetics 220 (4) (2022)
PUBMED 34897464
REFERENCE 2 (bases 1 to 576874)
AUTHORS Dietrich,F.S., Mulligan,J., Hennessy,K., Yelton,M.A., Allen,E.,
Araujo,R., Aviles,E., Berno,A., Brennan,T., Carpenter,J., Chen,E.,
Cherry,J.M., Chung,E., Duncan,M., Guzman,E., Hartzell,G.,
Hunicke-Smith,S., Hyman,R.W., Kayser,A., Komp,C., Lashkari,D.,
Lew,H., Lin,D., Mosedale,D., Davis,R.W. et al.
TITLE The nucleotide sequence of Saccharomyces cerevisiae chromosome V
JOURNAL Nature 387 (6632 SUPPL), 78-81 (1997)
PUBMED 9169868
REFERENCE 3 (bases 1 to 576874)
AUTHORS Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
Oliver,S.G.
TITLE Life with 6000 genes
JOURNAL Science 274 (5287), 546 (1996)
PUBMED 8849441
REFERENCE 4 (bases 1 to 576874)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (23-JAN-2026) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 5 (bases 1 to 576874)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (16-JAN-2015) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Protein update by submitter
REFERENCE 6 (bases 1 to 576874)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (29-JUL-2014) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Protein update by submitter
REFERENCE 7 (bases 1 to 576874)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (04-MAY-2012) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Protein update by submitter
REFERENCE 8 (bases 1 to 576874)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (31-MAR-2011) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Sequence update by submitter
REFERENCE 9 (bases 1 to 576874)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (11-DEC-2009) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
COMMENT REVIEWED REFSEQ: This record has been curated by SGD. The reference
sequence is identical to BK006939.
On Apr 26, 2011 this sequence version replaced NC_001137.2.
##Genome-Annotation-Data-START##
Annotation Provider :: SGD
Annotation Status :: Full Annotation
Annotation Version :: R64-4-1
URL :: http://www.yeastgenome.org/
##Genome-Annotation-Data-END##
COMPLETENESS: full length.
FEATURES Location/Qualifiers
source 1..576874
/organism="Saccharomyces cerevisiae S288C"
/mol_type="genomic DNA"
/strain="S288C"
/db_xref="taxon:559292"
/chromosome="V"
telomere complement(1..6473)
/note="TEL05L; Telomeric region on the left arm of
Chromosome V; annotated components include an X element
core sequence and a long Y' element; TEL05L does have
telomeric repeats (TEL05L-TR), but they are missing from
the genome annotation due to difficulties encountered
during sequencing and/or assembly"
/db_xref="SGD:S000028879"
gene complement(<264..>4097)
/locus_tag="YEL077C"
/db_xref="GeneID:856630"
mRNA complement(<264..>4097)
/locus_tag="YEL077C"
/product="Y' element ATP-dependent helicase"
/transcript_id="NM_001184302.1"
/db_xref="GeneID:856630"
CDS complement(264..4097)
/locus_tag="YEL077C"
/note="Helicase-like protein encoded within the telomeric
Y' element"
/codon_start=1
/product="Y' element ATP-dependent helicase"
/protein_id="NP_010835.1"
/db_xref="GeneID:856630"
/db_xref="SGD:S000006409"
/translation="MTLGNSYDAFNHDPWMDVVGFEDPNQVTNRDISRIVLYSYMFLN
TAKGCLVEYATFRQYMRELPKNAPQKLNFREMRQGLIALGRHCVGSRFETDLYESATS
ELMANHSVQTGRNIYGVDSFSLTSVSGTTATLLQERASERWIQWLGLESDYHCSFSST
RNAEDVVAGEAASSDHHQKISRVTRKRPREPKSTNDILVAGQKLFGSSFEFRDLHQLR
LCYEIYMADTPSVAVQAPPGYGKTELFHLPLIALASKGDVKYVSFLFVPYTVLLANCM
IRLGRCGCLNVAPVRNFIEEGYDGVTDLYVGIYDDLASTNFTDRIAAWENIVECTFRT
NNVKLGYLIVDEFHNFETEVYRQSQFGGITNLDFDAFEKAIFLSGTAPEAVADAALQR
IGLTGLAKKSMDINELKRSEDLSRGLSSYPTRMFNLIKEKSEVPLGHVHKIRKKVESQ
PEEALKLLLALFESEPESKAIVVASTTNEVEELACSWRKYFRVVWIHGKLDAAEKVSR
TKEFVTDGNMRVLIGTKLVTEGIDIKQLMMVIMLDNRLNIIELIQGVGRLRDGGLCYL
LSRKNSWAARNRKGELPPIKEGCITEQVREFYGLESKKGKKGQHVGCCGSRTDLSADT
VELIERMDRLAEKQATASMSIVALPSNFQESNSSDRYRKYCSSDEDSNTCIHGSANAS
TNASTNAITTASTNVRTNATTNASTNATTNASTNATTNSSTNATTTASTNVRTSATTT
ASINVRTSATTTESTNSSTNATTTASINVRTSATTTKSINSSTNATITESTNSNTNAT
TTESTNSKTSATTTASTNSNTSATTTESTNSKTSATTTASTNSNTSATTTESTNSNTS
ATTTASTNSSTNATTTESTNASAKEDANKDGNAEDNRFHPVTDINKESYKRKGSQMVL
LERKKLKAQFPNTSENMNVLQFLGFRSDEIKHLFLYGIDIYFCPEGVFTQYGLCKGCQ
KMFELCVCWAGQKVSYRRMAWEALAVERMLRNDEEYKEYLEDIEPYHGDPVGYLKYFS
VKRREIYSQIQRNYAWYLAITRRRETISVLDSTRGKQGSQVFRMSGRQIKELYYKVWS
NLRESKTEVLQYFLNWDEKKCQEEWEAKDDTVFVEALEKVGVFQRLRSMTSAGLQGPQ
YVKLQFSRHHRQLRSRYELSLGMHLRDQIALGVTPSKVPHWTAFLSMLIGLFYNKTFR
QKLEYLLEQISEVWLLPHWLDLANVEVLAADNTKVPLYMLMVAVHKELDSDDVPDGRF
DIILLCRDSSREVGE"
rep_origin 4097..4185
/note="ARS502; Autonomously Replicating Sequence"
/db_xref="SGD:S000077377"
gene complement(<4185..>5114)
/locus_tag="YEL076C-A"
/db_xref="GeneID:856632"
mRNA complement(join(<4185..4322,4602..>5114))
/locus_tag="YEL076C-A"
/product="uncharacterized protein"
/transcript_id="NM_001180855.1"
/db_xref="GeneID:856632"
CDS complement(join(4185..4322,4602..5114))
/locus_tag="YEL076C-A"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_010836.1"
/db_xref="GeneID:856632"
/db_xref="SGD:S000002955"
/translation="MQASLPGEKKVDTERLKRDLCPRKPIEIKYFSQICNDMMNKKDR
LGDILHIILRACALNFGAGPRGGAGDEEDRSITNEEPIIPSVDEHGLKVCKLRSPNTP
RRLRKTLDAVKALLVSSCACTARDLDIFDDNNGVAMWKWIKILYHEVAQETTLKDSYR
ITLVPSSDGISDTLTVIQSFSYSLLPVLSATYTSMIQQDASNCTLITTRTVHRSLD"
gene complement(<4464..>5114)
/locus_tag="YEL076C"
/db_xref="GeneID:856633"
mRNA complement(<4464..>5114)
/locus_tag="YEL076C"
/product="uncharacterized protein"
/transcript_id="NM_001178891.1"
/db_xref="GeneID:856633"
CDS complement(4464..5114)
/locus_tag="YEL076C"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_010837.1"
/db_xref="GeneID:856633"
/db_xref="SGD:S000000802"
/translation="MQASLPGEKKVDTERLKRDLCPRKPIEIKYFSQICNDMMNKKDR
LGDILHIILRACALNFGAGPRGGAGDEEDRSITNEEPIIPSVDEHGLKVCKLRSPNTP
RRLRKTLDAVKALLVSSCACTARDLDIFDDNNGVAMWKWIKILYHEVAQETTLKDSYR
ITLVPSSDGISVCGKLWGELNNCFHMPASFCVIEKFCSENRSEELTSIWKTILFFK"
gene complement(<5345..>5713)
/locus_tag="YEL075C"
/db_xref="GeneID:856634"
mRNA complement(<5345..>5713)
/locus_tag="YEL075C"
/product="uncharacterized protein"
/transcript_id="NM_001178890.1"
/db_xref="GeneID:856634"
CDS complement(5345..5713)
/locus_tag="YEL075C"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_010839.1"
/db_xref="GeneID:856634"
/db_xref="SGD:S000000801"
/translation="MKVSDRRKFEKANFDEFESALNNKNDLVHCPSITLFESIPTEVR
SFYEDEKSGLIKVVKFRTGAMDRKRSFEKVVISVMVGKNVKKFLTFVEDEPDFQGGPI
PSNKPRDGLHVVSSAYFEIQ"
rep_origin 6378..6453
/note="ARS503; Autonomously Replicating Sequence"
/db_xref="SGD:S000077378"
gene complement(<7230..>7553)
/locus_tag="YEL073C"
/db_xref="GeneID:856636"
mRNA complement(<7230..>7553)
/locus_tag="YEL073C"
/product="uncharacterized protein"
/transcript_id="NM_001178888.1"
/db_xref="GeneID:856636"
CDS complement(7230..7553)
/locus_tag="YEL073C"
/note="hypothetical protein; located adjacent to ARS503
and the telomere on the left arm of chromosome V;
regulated by inositol/choline"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_010841.1"
/db_xref="GeneID:856636"
/db_xref="SGD:S000000799"
/translation="MVNLANVLTNATAATLSAWSNTVPLETYFHFDEASGFGDYYLNV
SVIWMNETLYETRIVPAIINVREWLDHMEANDPSPSVTNPYETSGYYAFSTVVPVLMG
NMKVA"
rep_origin 8786..10019
/note="ARS504; Autonomously Replicating Sequence"
/db_xref="SGD:S000077379"
gene <13720..>14415
/gene="RMD6"
/locus_tag="YEL072W"
/db_xref="GeneID:856637"
mRNA <13720..>14415
/gene="RMD6"
/locus_tag="YEL072W"
/product="Rmd6p"
/transcript_id="NM_001178887.1"
/db_xref="GeneID:856637"
CDS 13720..14415
/gene="RMD6"
/locus_tag="YEL072W"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:35563734]"
/note="Protein required for sporulation; localizes to the
peroxisome"
/codon_start=1
/product="Rmd6p"
/protein_id="NP_010842.1"
/db_xref="GeneID:856637"
/db_xref="SGD:S000000798"
/translation="MSACPCNIVILPVEILKNSSKDTKYSLYTTINRGYDVPRLKYGI
IVSPRVHSLETLFSDLGFDKNIEKSSLYLLLNDPTLAYPNFHEHFEQLKGETNKDLSL
PTYYIPKVQFLTEAFDSEHTLATIGYKPNNKESYEITGFTSMGNGYGIKLFNYSVIHM
MRSHKCKRVVADIIMEHDLLGYYEKKLGFVEVQRFKVLKEQHQVKVFDDKVDFTKDFH
VIKMIKELGNHRL"
gene <16355..>17845
/gene="DLD3"
/locus_tag="YEL071W"
/db_xref="GeneID:856638"
mRNA <16355..>17845
/gene="DLD3"
/locus_tag="YEL071W"
/product="D-lactate dehydrogenase"
/transcript_id="NM_001178886.1"
/db_xref="GeneID:856638"
CDS 16355..17845
/gene="DLD3"
/locus_tag="YEL071W"
/EC_number="1.1.2.4"
/EC_number="1.1.99.40"
/experiment="EXISTENCE:direct assay:GO:0004458 D-lactate
dehydrogenase (cytochrome) activity [PMID:10509019]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276|PMID:10509019]"
/experiment="EXISTENCE:direct assay:GO:0071949 FAD binding
[PMID:26774271]"
/experiment="EXISTENCE:direct assay:GO:0099615
(R)-2-hydroxyglutarate-pyruvate transhydrogenase activity
[PMID:26774271]"
/experiment="EXISTENCE:mutant phenotype:GO:0019249 lactate
biosynthetic process [PMID:26774271]"
/experiment="EXISTENCE:mutant phenotype:GO:0030447
filamentous growth [PMID:16999827]"
/experiment="EXISTENCE:mutant phenotype:GO:0099615
(R)-2-hydroxyglutarate-pyruvate transhydrogenase activity
[PMID:26774271]"
/note="2-hydroxyglutarate transhydrogenase, and minor
D-lactate dehydrogenase; converts D-2-hydroxyglutarate
(D-2HG), an oncometabolite, to alpha-ketoglutarate in the
presence of FAD, with concomitant reduction of pyruvate to
D-lactate; minor lactate dehydrogenase activity; component
of the retrograde regulon that consists of genes whose
expression are stimulated by damage to mitochondria and
reduced in cells grown with glutamate as the sole nitrogen
source; located in the cytoplasm"
/codon_start=1
/product="D-lactate dehydrogenase"
/protein_id="NP_010843.1"
/db_xref="GeneID:856638"
/db_xref="SGD:S000000797"
/translation="MTAAHPVAQLTAEAYPKVKRNPNFKVLDSEDLAYFRSILSNDEI
LNSQAPEELASFNQDWMKKYRGQSNLILLPNSTDKVSKIMKYCNDKKLAVVPQGGNTD
LVGASVPVFDEIVLSLRNMNKVRDFDPVSGTFKCDAGVVMRDAHQFLHDHDHIFPLDL
PSRNNCQVGGVVSTNAGGLNFLRYGSLHGNVLGLEVVLPNGEIISNINALRKDNTGYD
LKQLFIGAEGTIGVVTGVSIVAAAKPKALNAVFFGIENFDTVQKLFVKAKSELSEILS
AFEFMDRGSIECTIEYLKDLPFPLENQHNFYVLIETSGSNKRHDDEKLTAFLKDTTDS
KLISEGMMAKDKADFDRLWTWRKSVPTACNSYGGMYKYDMSLQLKDLYSVSAAVTERL
NAAGLIGDAPKPVVKSCGYGHVGDGNIHLNIAVREFTKQIEDLLEPFVYEYIASKKGS
ISAEHGIGFHKKGKLHYTRSDIEIRFMKDIKNHYDPNGILNPYKYI"
gene <19589..>21097
/gene="DSF1"
/locus_tag="YEL070W"
/gene_synonym="MAN1"
/db_xref="GeneID:856639"
mRNA <19589..>21097
/gene="DSF1"
/locus_tag="YEL070W"
/gene_synonym="MAN1"
/product="mannitol dehydrogenase DSF1"
/transcript_id="NM_001178885.1"
/db_xref="GeneID:856639"
CDS 19589..21097
/gene="DSF1"
/locus_tag="YEL070W"
/gene_synonym="MAN1"
/EC_number="1.1.1.67"
/experiment="EXISTENCE:mutant phenotype:GO:0046029
mannitol dehydrogenase activity [PMID:26996892]"
/note="Mannitol dehydrogenase; deletion suppresses
mutation of mpt5; DSF1 has a paralog, MAN2, that arose
from a segmental duplication"
/codon_start=1
/product="mannitol dehydrogenase DSF1"
/protein_id="NP_010844.1"
/db_xref="GeneID:856639"
/db_xref="SGD:S000000796"
/translation="MTKSDETTATSLNAKTLKSFESTLPIPTYPREGVKQGIVHLGVG
AFHRSHLAVFMHRLMQEHHLKDWSICGVGLMKADALMRDAMKAQDCLYTLVERGIKDT
NAYIVGSITAYMYAPDDPRAVIEKMANPDTHIVSLTVTENGYYHSEATNSLMTDAPEI
INDLNHPEKPDTLYGYLYEALLLRYKRGLTPFTIMSCDNMPQNGVTVKTMLVAFAKLK
KDEKFAAWIEDKVTSPNSMVDRVTPRCTDKERKYVADTWGIKDQCPVVAEPFIQWVLE
DNFSDGRPPWELVGVQVVKDVDSYELMKLRLLNGGHSAMGYLGYLAGYTYIHEVVNDP
TINKYIRVLMREEVIPLLPKVPGVDFEEYTASVLERFSNPAIQDTVARICLMGSGKMP
KYVLPSIYEQLRKPDGKYKLLAVCVAGWFRYLTGVDMNGKPFEIEDPMAPTLKAAAVK
GGKDPHELLNIEVLFSPEIRDNKEFVAQLTHSLETVYDKGPIAAIKEILDQV"
gene complement(<21537..>23231)
/gene="HXT13"
/locus_tag="YEL069C"
/gene_synonym="HLT1"
/db_xref="GeneID:856640"
mRNA complement(<21537..>23231)
/gene="HXT13"
/locus_tag="YEL069C"
/gene_synonym="HLT1"
/product="hexose transporter HXT13"
/transcript_id="NM_001178884.1"
/db_xref="GeneID:856640"
CDS complement(21537..23231)
/gene="HXT13"
/locus_tag="YEL069C"
/gene_synonym="HLT1"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0015795
sorbitol transmembrane transport [PMID:26996892]"
/experiment="EXISTENCE:genetic interaction:GO:0015797
mannitol transmembrane transport [PMID:26996892]"
/experiment="EXISTENCE:mutant phenotype:GO:0005353
fructose transmembrane transporter activity
[PMID:10618490]"
/experiment="EXISTENCE:mutant phenotype:GO:0008645 hexose
transmembrane transport [PMID:10618490]"
/experiment="EXISTENCE:mutant phenotype:GO:0015578 mannose
transmembrane transporter activity [PMID:10618490]"
/experiment="EXISTENCE:mutant phenotype:GO:0055056
D-glucose transmembrane transporter activity
[PMID:10618490]"
/note="Putative transmembrane polyol transporter; supports
growth on and uptake of mannitol and sorbitol with
moderate affinity when overexpressed in a strain deleted
for hexose family members; minor hexose transport activity
when overexpressed in a similar strain; induced by
non-fermentable carbon sources; induced in low glucose,
repressed in high glucose; HXT13 has a paralog, HXT17,
that arose from a segmental duplication"
/codon_start=1
/product="hexose transporter HXT13"
/protein_id="NP_010845.1"
/db_xref="GeneID:856640"
/db_xref="SGD:S000000795"
/translation="MSSAQSSIDSDGDVRDADIHVAPPVEKEWSDGFDDNEVINGDNV
EPPKRGLIGYLVIYLLCYPISFGGFLPGWDSGITAGFINMDNFKMNFGSYKHSTGEYY
LSNVRMGLLVAMFSIGCAIGGLIFARLADTLGRRLAIVIVVLVYMVGAIIQISSNHKW
YQYFVGKIIYGLGAGGCSVLCPMLLSEIAPTDLRGGLVSLYQLNMTFGIFLGYCSVYG
TRKYDNTAQWRVPLGLCFLWALIIIIGMLLVPESPRYLIECERHEEARASIAKINKVS
PEDPWVLKQADEINAGVLAQRELGEASWKELFSVKTKVLQRLITGILVQTFLQLTGEN
YFFFYGTTIFKSVGLTDGFETSIVLGTVNFFSTIIAVMVVDKIGRRKCLLFGAAGMMA
CMVIFASIGVKCLYPHGQDGPSSKGAGNAMIVFTCFYIFCFATTWAPVAYIVVAESFP
SKVKSRAMSISTACNWLWQFLIGFFTPFITGSIHFYYGYVFVGCLVAMFLYVFFFLPE
TIGLSLEEIQLLYEEGIKPWKSASWVPPSRRGISSEESKTEKKDWKKFLKFSKNSD"
gene complement(<25646..>25978)
/locus_tag="YEL068C"
/db_xref="GeneID:856641"
mRNA complement(<25646..>25978)
/locus_tag="YEL068C"
/product="uncharacterized protein"
/transcript_id="NM_001270743.1"
/db_xref="GeneID:856641"
CDS complement(25646..25978)
/locus_tag="YEL068C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/note="hypothetical protein; expressed at both mRNA and
protein levels; SWAT-GFP and seamless-GFP fusion proteins
localize to the endoplasmic reticulum and mCherry fusion
protein localizes to the vacuole"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001257672.1"
/db_xref="GeneID:856641"
/db_xref="SGD:S000000794"
/translation="MKILALAVAFQGLALVCCTDTTDNYNNFTHNTSTFYRIADYDKR
FWSVKLPPVNWTTCGNEDHPWITIHYDEIDKLVDDKLASWDKGPVPDREEFRNSILRQ
ARCDRPHY"
gene complement(<26189..>26776)
/locus_tag="YEL067C"
/db_xref="GeneID:856643"
mRNA complement(<26189..>26776)
/locus_tag="YEL067C"
/product="uncharacterized protein"
/transcript_id="NM_001178882.1"
/db_xref="GeneID:856643"
CDS complement(26189..26776)
/locus_tag="YEL067C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/note="hypothetical protein; the authentic, non-tagged
protein is detected in highly purified mitochondria in
high-throughput studies"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_010847.1"
/db_xref="GeneID:856643"
/db_xref="SGD:S000000793"
/translation="MAFTTIRSLTIFSGSSFDMPTFFVLAKGGDGSGVFFISLHSSIL
YARFPLLNILSQHSPVYCSRRFPPDNDLITGLGNIRCGQLVKKALRRVCNVPPDHQLI
ASVRSIGYARVAFVFCTCTSECRDNLQSVTRTSGHQTTISQPCTDIQSTRKCSTIFVS
AVMQTGTITFFWTFHFPIPLQRIVNQKQPPPQSYV"
gene <26721..>27206
/gene="HPA3"
/locus_tag="YEL066W"
/db_xref="GeneID:856642"
mRNA <26721..>27206
/gene="HPA3"
/locus_tag="YEL066W"
/product="D-amino-acid N-acetyltransferase"
/transcript_id="NM_001178881.2"
/db_xref="GeneID:856642"
CDS 26721..27206
/gene="HPA3"
/locus_tag="YEL066W"
/EC_number="2.3.1.36"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006473 protein
acetylation [PMID:23775086]"
/experiment="EXISTENCE:direct assay:GO:0008080
N-acetyltransferase activity [PMID:23775086]"
/experiment="EXISTENCE:direct assay:GO:0047812
D-amino-acid N-acetyltransferase activity [PMID:15375647]"
/experiment="EXISTENCE:mutant phenotype:GO:0046416 D-amino
acid metabolic process [PMID:16362288]"
/experiment="EXISTENCE:mutant phenotype:GO:0070458
cellular detoxification of nitrogen compound
[PMID:23775086|PMID:16362288]"
/note="D-Amino acid N-acetyltransferase that detoxifies
D-amino acids; catalyzes N-acetylation of D-amino acids
through ordered bi-bi mechanism in which acetyl-CoA is
first substrate bound and CoA is last product liberated;
acetylates histones and polyamines, also autoacetylates"
/codon_start=1
/product="D-amino-acid N-acetyltransferase"
/protein_id="NP_010848.2"
/db_xref="GeneID:856642"
/db_xref="SGD:S000000792"
/translation="MSNEEPEKMVNDRIVVKAIEPKDEEAWNKLWKEYQGFQKTVMPP
EVATTTFARFIDPTVKLWGALAFDTETGDAIGFAHYLNHLTSWHVEEVVYMNDLYVTE
RARVKGVGRKLIEFVYSRADELGTPAVYWVTDHYNHRAQLLYTKVAYKTDKVLYKRNG
Y"
gene <27657..>29543
/gene="SIT1"
/locus_tag="YEL065W"
/gene_synonym="ARN3"
/db_xref="GeneID:856644"
mRNA <27657..>29543
/gene="SIT1"
/locus_tag="YEL065W"
/gene_synonym="ARN3"
/product="siderophore transporter"
/transcript_id="NM_001178880.3"
/db_xref="GeneID:856644"
CDS 27657..29543
/gene="SIT1"
/locus_tag="YEL065W"
/gene_synonym="ARN3"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:19469713]"
/experiment="EXISTENCE:direct assay:GO:0006879
intracellular iron ion homeostasis [PMID:9884238]"
/experiment="EXISTENCE:direct assay:GO:0031410 cytoplasmic
vesicle [PMID:10748025]"
/experiment="EXISTENCE:mutant phenotype:GO:0006879
intracellular iron ion homeostasis [PMID:9884238]"
/experiment="EXISTENCE:mutant phenotype:GO:0015344
siderophore uptake transmembrane transporter activity
[PMID:9884238]"
/experiment="EXISTENCE:mutant phenotype:GO:0015891
siderophore transport [PMID:9884238]"
/experiment="EXISTENCE:mutant phenotype:GO:0055085
transmembrane transport [PMID:9884238]"
/note="Ferrioxamine B transporter; member of the ARN
family of transporters that specifically recognize
siderophore-iron chelates; transcription is induced during
iron deprivation and diauxic shift; potentially
phosphorylated by Cdc28p"
/codon_start=1
/product="siderophore transporter"
/protein_id="NP_010849.3"
/db_xref="GeneID:856644"
/db_xref="SGD:S000000791"
/translation="MDPGIANHTLPEEFEEVVVPEMLEKEVGAKVDVKPTLTTSSPAP
SYIELIDPGVHNIEIYAEMYNRPIYRVALFFSLFLIAYAYGLDGNIRYTFQAYATSSY
SQHSLLSTVNCIKTVIAAVGQIFFARLSDIFGRFSIMIVSIIFYSMGTIIESQAVNIT
RFAVGGCFYQLGLTGIILILEVIASDFSNLNWRLLALFIPALPFIINTWISGNVTSAI
DANWKWGIGMWAFILPLACIPLGICMLHMRYLARKHAKDRLKPEFEALNKLKWKSFCI
DIAFWKLDIIGMLLITVFFGCVLVPFTLAGGLKEEWKTAHIIVPEVIGWVVVLPLYML
WEIKYSRHPLTPWDLIQDRGIFFALLIAFFINFNWYMQGDYMYTVLVVAVHESIKSAT
RITSLYSFVSVIVGTILGFILIKVRRTKPFIIFGISCWIVSFGLLVHYRGDSGAHSGI
IGSLCLLGFGAGSFTYVTQASIQASAKTHARMAVVTSLYLATYNIGSAFGSSVSGAVW
TNILPKEISKRISDPTLAAQAYGSPFTFITTYTWGTPERIALVMSYRYVQKILCIIGL
VFCFPLLGCAFMLRNHKLTDSIALEGNDHLESKNTFEIEEKEESFLKNKFFTHFTSSK
DRKD"
gene complement(<29797..>31239)
/gene="AVT2"
/locus_tag="YEL064C"
/db_xref="GeneID:856645"
mRNA complement(<29797..>31239)
/gene="AVT2"
/locus_tag="YEL064C"
/product="Avt2p"
/transcript_id="NM_001178879.1"
/db_xref="GeneID:856645"
CDS complement(29797..31239)
/gene="AVT2"
/locus_tag="YEL064C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:11274162]"
/note="Putative transporter; member of a family of seven
S. cerevisiae genes (AVT1-7) related to vesicular
GABA-glycine transporters"
/codon_start=1
/product="Avt2p"
/protein_id="NP_010850.1"
/db_xref="GeneID:856645"
/db_xref="SGD:S000000790"
/translation="MSELGEYSKLENKELRTEFELTNFPFPGTTDNDSDDGSQGQNSL
NIITPDMDDTLVNDVLRENDKKSSMRMAFMNLANSILGAGIITQPFAIKNAGILGGLL
SYVALGFIVDWTLRLIVINLTLAGKRTYQGTVEHVMGKKGKLLILFTNGLFAFGGCIG
YCIIIGDTIPHVLRAIFSQNDGNVHFWLRRNVIIVMVTTFISFPLSMKRNIEALSKAS
FLAVISMIIIVLTVVIRGPMLPYDWKGHSLKLSDFFMKATIFRSLSVISFALVCHHNT
SFIFFSMRNRSVAKFTRLTHISIIISVICCALMGYSGFAVFKEKTKGNVLNSFPGTDT
AINIARLCFGFNMLTTFPMEIFVLRDVVGNLLHECNLIKNYDEHTQLSGKQHVVITSS
LVFITMGISLTTCNLGALFELIGATTASTMAYILPPYTNLLLTSKKKSWKERLPFYLC
ICFGFMIMIISSTQTIIDAVNGSDGQHCQI"
gene complement(<31694..>33466)
/gene="CAN1"
/locus_tag="YEL063C"
/db_xref="GeneID:856646"
mRNA complement(<31694..>33466)
/gene="CAN1"
/locus_tag="YEL063C"
/product="arginine permease CAN1"
/transcript_id="NM_001178878.1"
/db_xref="GeneID:856646"
CDS complement(31694..33466)
/gene="CAN1"
/locus_tag="YEL063C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:15536122]"
/experiment="EXISTENCE:direct assay:GO:0015174 basic amino
acid transmembrane transporter activity [PMID:10654085]"
/experiment="EXISTENCE:direct assay:GO:0015802 basic amino
acid transport [PMID:10654085]"
/experiment="EXISTENCE:direct assay:GO:0032126 eisosome
[PMID:15536122|PMID:29402931]"
/experiment="EXISTENCE:direct assay:GO:0055085
transmembrane transport [PMID:10654085]"
/experiment="EXISTENCE:genetic interaction:GO:1903826
L-arginine transmembrane transport [PMID:23047103]"
/experiment="EXISTENCE:mutant phenotype:GO:0061459
L-arginine transmembrane transporter activity
[PMID:10753927]"
/note="Plasma membrane arginine permease; transceptor that
senses extracellular basic amino acids; negative regulator
of biofilm formation; requires phosphatidyl ethanolamine
(PE) for localization, exclusively associated with lipid
rafts; mutation confers canavanine resistance; CAN1 has a
paralog, ALP1, that arose from the whole genome
duplication"
/codon_start=1
/product="arginine permease CAN1"
/protein_id="NP_010851.1"
/db_xref="GeneID:856646"
/db_xref="SGD:S000000789"
/translation="MTNSKEDADIEEKHMYNEPVTTLFHDVEASQTHHRRGSIPLKDE
KSKELYPLRSFPTRVNGEDTFSMEDGIGDEDEGEVQNAEVKRELKQRHIGMIALGGTI
GTGLFIGLSTPLTNAGPVGALISYLFMGSLAYSVTQSLGEMATFIPVTSSFTVFSQRF
LSPAFGAANGYMYWFSWAITFALELSVVGQVIQFWTYKVPLAAWISIFWVIITIMNLF
PVKYYGEFEFWVASIKVLAIIGFLIYCFCMVCGAGVTGPVGFRYWRNPGAWGPGIISK
DKNEGRFLGWVSSLINAAFTFQGTELVGITAGEAANPRKSVPRAIKKVVFRILTFYIG
SLLFIGLLVPYNDPKLTQSTSYVSTSPFIIAIENSGTKVLPHIFNAVILTTIISAANS
NIYVGSRILFGLSKNKLAPKFLSRTTKGGVPYIAVFVTAAFGALAYMETSTGGDKVFE
WLLNITGVAGFFAWLFISISHIRFMQALKYRGISRDELPFKAKLMPGLAYYAATFMTI
IIIIQGFTAFAPKFNGVSFAAAYISIFLFLAVWILFQCIFRCRFIWKIGDVDIDSDRR
DIEAIVWEDHEPKTFWDKFWNVVA"
gene <34407..>36254
/gene="NPR2"
/locus_tag="YEL062W"
/db_xref="GeneID:856647"
mRNA <34407..>36254
/gene="NPR2"
/locus_tag="YEL062W"
/product="nitrogen permease regulating protein NPR2"
/transcript_id="NM_001178877.3"
/db_xref="GeneID:856647"
CDS 34407..36254
/gene="NPR2"
/locus_tag="YEL062W"
/experiment="EXISTENCE:direct assay:GO:0035859
Seh1-associated complex [PMID:21454883]"
/experiment="EXISTENCE:direct assay:GO:1990130 GATOR1
complex [PMID:21900499]"
/experiment="EXISTENCE:genetic interaction:GO:0006995
cellular response to nitrogen starvation [PMID:25046117]"
/experiment="EXISTENCE:genetic interaction:GO:0010508
positive regulation of autophagy [PMID:25046117]"
/experiment="EXISTENCE:genetic interaction:GO:1904262
negative regulation of TORC1 signaling
[PMID:25046117|PMID:23716719]"
/experiment="EXISTENCE:mutant phenotype:GO:0006995
cellular response to nitrogen starvation [PMID:25046117]"
/experiment="EXISTENCE:mutant phenotype:GO:0009410
response to xenobiotic stimulus [PMID:12869630]"
/experiment="EXISTENCE:mutant phenotype:GO:0010508
positive regulation of autophagy [PMID:25046117]"
/experiment="EXISTENCE:mutant phenotype:GO:0015824 proline
transport [PMID:7867803]"
/experiment="EXISTENCE:mutant phenotype:GO:0015840 urea
transport [PMID:7867803]"
/experiment="EXISTENCE:mutant phenotype:GO:0034198
cellular response to amino acid starvation
[PMID:19521502]"
/experiment="EXISTENCE:mutant phenotype:GO:1904262
negative regulation of TORC1 signaling
[PMID:25046117|PMID:19521502|PMID:23716719]"
/experiment="EXISTENCE:mutant phenotype:GO:2000785
regulation of autophagosome assembly [PMID:21900499]"
/note="Subunit of the Iml1p/SEACIT complex; SEACIT
(Iml1p-Npr2p-Npr3p) is a subcomplex of the SEA complex, a
coatomer-related complex that associates dynamically with
the vacuole; Npr2p may have a structural or regulatory
role, supporting Iml1p function as a GAP for the Rag
family GTPase Gtr1p, and resulting in inhibition of TORC1
signaling in response to amino acid deprivation; SEACIT is
required for non-nitrogen-starvation-induced autophagy;
homolog of human tumor suppressor NPRL2"
/codon_start=1
/product="nitrogen permease regulating protein NPR2"
/protein_id="NP_010852.4"
/db_xref="GeneID:856647"
/db_xref="SGD:S000000788"
/translation="MLSYFQGFVPIHTIFYSVFHPTEGSKIKYEFPPNNLKNHGINFN
TFKNYIIPKPILCHKLITFKYGTYRIVCYPVTINSPIYARNFFSFNFVFVFPYDCETS
PYEPAITRLGKMFKVLEEQNQLLSKSERDPVFFDLKVLENSTTTPSTAGPSSTPNPSS
NTTPTHPTSEKDTKDMRSSRYSDLIKDLGLPQSAFSIQDLLMRIFQDLNNYSECLIPI
DEGNAVDIKIFPLLRPPTTCVSLEDVPLSSVNLKKIIDVNWDPTMMSIVPYIDGLNSI
AKISKLSNSDPGLVIECIRHLIYYKCVTLSDIFQFSNIYAPSSLIRNFLTDPLMASDC
QSYVTFPEVSKISNLPLNKSLGSGDQDSPSFSVRRKSKSSSIPSNPDSRTTSFSSTSR
VSQNSSLNSSFSSIYKDWRQSQTSCSSSNIHVINNRNRFLPTRSCLFDLYRSLSQGQT
LKTWYESKYMILKENNIDIRRFITFGLEKRIIYRCYSFPVMINAGSREPKEMTPIITK
DLVNNDKLLEKRNHNHLLSATGSRNTAQSGNLKPERPSKVSFEMQRVSSLATGKSTMP
KLSDEEEGILEESIRNAETFDKICVLLSKPKLEVESYLNELGEFKVINS"
gene complement(<36535..>39537)
/gene="CIN8"
/locus_tag="YEL061C"
/gene_synonym="KSL2; SDS15"
/db_xref="GeneID:856648"
mRNA complement(<36535..>39537)
/gene="CIN8"
/locus_tag="YEL061C"
/gene_synonym="KSL2; SDS15"
/product="kinesin motor protein CIN8"
/transcript_id="NM_001178876.1"
/db_xref="GeneID:856648"
CDS complement(36535..39537)
/gene="CIN8"
/locus_tag="YEL061C"
/gene_synonym="KSL2; SDS15"
/experiment="EXISTENCE:direct assay:GO:0000776 kinetochore
[PMID:11511347]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005828 kinetochore
microtubule [PMID:19041752]"
/experiment="EXISTENCE:direct assay:GO:0005876 spindle
microtubule [PMID:11511347|PMID:1618897]"
/experiment="EXISTENCE:direct assay:GO:0008569
minus-end-directed microtubule motor activity
[PMID:21350123]"
/experiment="EXISTENCE:direct assay:GO:0008574
plus-end-directed microtubule motor activity
[PMID:21350123]"
/experiment="EXISTENCE:direct assay:GO:0033047 regulation
of mitotic sister chromatid segregation [PMID:31253793]"
/experiment="EXISTENCE:direct assay:GO:1990023 mitotic
spindle midzone [PMID:16533946]"
/experiment="EXISTENCE:genetic interaction:GO:0000070
mitotic sister chromatid segregation [PMID:7860634]"
/experiment="EXISTENCE:genetic interaction:GO:0000073
initial mitotic spindle pole body separation
[PMID:1618910|PMID:1618897]"
/experiment="EXISTENCE:genetic interaction:GO:0003777
microtubule motor activity [PMID:1618910]"
/experiment="EXISTENCE:genetic interaction:GO:0098653
centromere clustering [PMID:16533946]"
/experiment="EXISTENCE:mutant phenotype:GO:0000022 mitotic
spindle elongation [PMID:23851487|PMID:9813090]"
/experiment="EXISTENCE:mutant phenotype:GO:0000073 initial
mitotic spindle pole body separation [PMID:1618897]"
/experiment="EXISTENCE:mutant phenotype:GO:0000235 astral
microtubule [PMID:19041752]"
/experiment="EXISTENCE:mutant phenotype:GO:0007019
microtubule depolymerization [PMID:19041752]"
/experiment="EXISTENCE:mutant phenotype:GO:0008569
minus-end-directed microtubule motor activity
[PMID:27216310]"
/experiment="EXISTENCE:mutant phenotype:GO:0045144 meiotic
sister chromatid segregation [PMID:31964755]"
/experiment="EXISTENCE:mutant phenotype:GO:0090307 mitotic
spindle assembly [PMID:9813090|PMID:23851487]"
/experiment="EXISTENCE:mutant phenotype:GO:0098653
centromere clustering [PMID:16533946]"
/note="Bipolar kinesin motor protein; switches from minus-
to plus-end-directed motility in vitro depending on
conditions; involved in mitotic spindle assembly,
chromosome segregation and centromere clustering on the
metaphase spindle"
/codon_start=1
/product="kinesin motor protein CIN8"
/protein_id="NP_010853.2"
/db_xref="GeneID:856648"
/db_xref="SGD:S000000787"
/translation="MPAENQNTGQDRSSNSISKNGNSQVGCHTVPNEELNITVAVRCR
GRNEREISMKSSVVVNVPDITGSKEISINTTGDTGITAQMNAKRYTVDKVFGPGASQD
LIFDEVAGPLFQDFIKGYNCTVLVYGMTSTGKTYTMTGDEKLYNGELSDAAGIIPRVL
LKLFDTLELQQNDYVVKCSFIELYNEELKDLLDSNSNGSSNTGFDGQFMKKLRIFDSS
TANNTTSNSASSSRSNSRNSSPRSLNDLTPKAALLRKRLRTKSLPNTIKQQYQQQQAV
NSRNNSSSNSGSTTNNASSNTNTNNGQRSSMAPNDQTNGIYIQNLQEFHITNAMEGLN
LLQKGLKHRQVASTKMNDFSSRSHTIFTITLYKKHQDELFRISKMNLVDLAGSENINR
SGALNQRAKEAGSINQSLLTLGRVINALVDKSGHIPFRESKLTRLLQDSLGGNTKTAL
IATISPAKVTSEETCSTLEYASKAKNIKNKPQLGSFIMKDILVKNITMELAKIKSDLL
STKSKEGIYMSQDHYKNLNSDLESYKNEVQECKREIESLTSKNALLVKDKLKSKETIQ
SQNCQIESLKTTIDHLRAQLDKQHKTEIEISDFNNKLQKLTEVMQMALHDYKKRELDL
NQKFEMHITKEIKKLKSTLFLQLNTMQQESILQETNIQPNLDMIKNEVLTLMRTMQEK
AELMYKDCVKKILNESPKFFNVVIEKIDIIRVDFQKFYKNIAENLSDISEENNNMKQY
LKNHFFKNNHQELLNRHVDSTYENIEKRTNEFVENFKKVLNDHLDENKKLIMQNLTTA
TSAVIDQEMDLFEPKRVKWENSFDLINDCDSMNNEFYNSMAATLSQIKSTVDTSSNSM
NESISVMKGQVEESENAISLLKNNTKFNDQFEQLINKHNMLKDNIKNSITSTHSHITN
VDDIYNTIENIMKNYGNKENATKDEMIENILKEIPNLSKKMPLRLSNINSNSVQSVIS
PKKHAIEDENKSSENVDNEGSRKMLKIE"
gene complement(<40046..>41953)
/gene="PRB1"
/locus_tag="YEL060C"
/gene_synonym="CVT1"
/db_xref="GeneID:856649"
mRNA complement(<40046..>41953)
/gene="PRB1"
/locus_tag="YEL060C"
/gene_synonym="CVT1"
/product="proteinase B"
/transcript_id="NM_001178875.1"
/db_xref="GeneID:856649"
CDS complement(40046..41953)
/gene="PRB1"
/locus_tag="YEL060C"
/gene_synonym="CVT1"
/EC_number="3.4.21.48"
/experiment="EXISTENCE:mutant phenotype:GO:0000425
pexophagy [PMID:31332264]"
/experiment="EXISTENCE:mutant phenotype:GO:0004252
serine-type endopeptidase activity
[PMID:377296|PMID:385314]"
/experiment="EXISTENCE:mutant phenotype:GO:0007039 protein
catabolic process in the vacuole
[PMID:2674123|PMID:385314]"
/experiment="EXISTENCE:mutant phenotype:GO:0030435
sporulation resulting in formation of a cellular spore
[PMID:2674123]"
/note="Vacuolar proteinase B yscB with H3 N-terminal
endopeptidase activity; serine protease of subtilisin
family; involved in protein degradation in vacuole;
required for full protein degradation during sporulation;
activity inhibited by Pbi2p; protein abundance increases
in response to DNA replication stress; targeted to vacuole
via Vps10p-dependent endosomal vacuolar protein sorting
pathway and via AP-3 pathway; PRB1 has a paralog, YSP3,
that arose from the whole genome duplication"
/codon_start=1
/product="proteinase B"
/protein_id="NP_010854.1"
/db_xref="GeneID:856649"
/db_xref="SGD:S000000786"
/translation="MKLENTLFTLGALGSISAALVIPNLENAADHHELINKEDHHERP
RKVEFTKDDDEEPSDSEDKEHGKFHKKGRKGQDKESPEFNGKRASGSHGSAHEGGKGM
KPKHESSNDDDNDDKKKKPHHKGGCHENKVEEKKMKGKKVKGKKHHEKTLEKGRHHNR
LAPLVSTAQFNPDAISKIIPNRYIIVFKRGAPQEEIDFHKENVQQAQLQSVENLSAED
AFFISTKDTSLSTSEAGGIQDSFNIDNLFSGYIGYFTQEIVDLIRQNPLVDFVERDSI
VEATEFDTQNSAPWGLARISHRERLNLGSFNKYLYDDDAGRGVTSYVIDTGVNINHKD
FEKRAIWGKTIPLNDEDLDGNGHGTHCAGTIASKHYGVAKNANVVAVKVLRSNGSGTM
SDVVKGVEYAAKAHQKEAQEKKKGFKGSTANMSLGGGKSPALDLAVNAAVEVGIHFAV
AAGNENQDACNTSPASADKAITVGASTLSDDRAYFSNWGKCVDVFAPGLNILSTYIGS
DDATATLSGTSMASPHVAGLLTYFLSLQPGSDSEFFELGQDSLTPQQLKKKLIHYSTK
DILFDIPEDTPNVLIYNGGGQDLSAFWNDTKKSHSSGFKQELNMDEFIGSKTDLIFDQ
VRDILDKLNII"
gene complement(<42400..>42624)
/gene="SOM1"
/locus_tag="YEL059C-A"
/db_xref="GeneID:856650"
mRNA complement(<42400..>42624)
/gene="SOM1"
/locus_tag="YEL059C-A"
/product="Som1p"
/transcript_id="NM_001180854.3"
/db_xref="GeneID:856650"
CDS complement(42400..42624)
/gene="SOM1"
/locus_tag="YEL059C-A"
/experiment="EXISTENCE:curator inference:GO:0004175
endopeptidase activity [PMID:10821182]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:9604886]"
/experiment="EXISTENCE:mutant phenotype:GO:0033108
mitochondrial respiratory chain complex assembly
[PMID:19703468]"
/experiment="EXISTENCE:physical interaction:GO:0042720
mitochondrial inner membrane peptidase complex
[PMID:10821182]"
/note="Subunit of the mitochondrial inner membrane
peptidase (IMP); IMP is required for maturation of
mitochondrial proteins of the intermembrane space; Som1p
facilitates cleavage of a subset of substrates; contains
twin cysteine-x9-cysteine motifs"
/codon_start=1
/product="Som1p"
/protein_id="NP_058154.3"
/db_xref="GeneID:856650"
/db_xref="SGD:S000002954"
/translation="MAPPTTIRTRDQALAPLATLDSQTNCRLKELVQWECQFKGAEYV
CSPFKRLFEHCIAPDKSATNYEVTDTYTNS"
gene <42652..>42960
/gene="HHY1"
/locus_tag="YEL059W"
/db_xref="GeneID:856651"
mRNA <42652..>42960
/gene="HHY1"
/locus_tag="YEL059W"
/product="Hhy1p"
/transcript_id="NM_001395033.1"
/db_xref="GeneID:856651"
CDS 42652..42960
/gene="HHY1"
/locus_tag="YEL059W"
/note="hypothetical protein; null mutant is sensitive to
heat, pH disturbances, ionic stress, and various
chemicals; hypersensitivity to hygromycin B indicative of
defects in vacuolar trafficking"
/codon_start=1
/product="Hhy1p"
/protein_id="NP_001381963.1"
/db_xref="GeneID:856651"
/db_xref="SGD:S000000785"
/translation="MSLSFLLFSPFLPPCFSSISICLSVLSTVSFFFAFTIPHYVLRC
GSVDEWHIHSSAEDFRTQRCVCAVKLSASLLGCLLACASWSLLLEVSRIKWHVGTAYS
"
gene <43252..>44925
/gene="PCM1"
/locus_tag="YEL058W"
/gene_synonym="AGM1"
/db_xref="GeneID:856652"
mRNA <43252..>44925
/gene="PCM1"
/locus_tag="YEL058W"
/gene_synonym="AGM1"
/product="phosphoacetylglucosamine mutase PCM1"
/transcript_id="NM_001178873.1"
/db_xref="GeneID:856652"
CDS 43252..44925
/gene="PCM1"
/locus_tag="YEL058W"
/gene_synonym="AGM1"
/EC_number="5.4.2.3"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:11914276]"
/experiment="EXISTENCE:mutant phenotype:GO:0004610
phosphoacetylglucosamine mutase activity [PMID:8174553]"
/experiment="EXISTENCE:mutant phenotype:GO:0006031 chitin
biosynthetic process [PMID:8174553]"
/note="Essential N-acetylglucosamine-phosphate mutase;
converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor
for the biosynthesis of chitin and for the formation of
N-glycosylated mannoproteins and
glycosylphosphatidylinositol anchors"
/codon_start=1
/product="phosphoacetylglucosamine mutase PCM1"
/protein_id="NP_010856.1"
/db_xref="GeneID:856652"
/db_xref="SGD:S000000784"
/translation="MKVDYEQLCKLYDDTCRTKNVQFSYGTAGFRTLAKNLDTVMFST
GILAVLRSLKLQGQYVGVMITASHNPYQDNGVKIVEPDGSMLLATWEPYAMQLANAAS
FATNFEEFRVELAKLIEHEKIDLNTTVVPHIVVGRDSRESSPYLLRCLTSSMASVFHA
QVLDLGCVTTPQLHYITDLSNRRKLEGDTAPVATEQDYYSFFIGAFNELFATYQLEKR
LSVPKLFIDTANGIGGPQLKKLLASEDWDVPAEQVEVINDRSDVPELLNFECGADYVK
TNQRLPKGLSPSSFDSLYCSFDGDADRVVFYYVDSGSKFHLLDGDKISTLFAKFLSKQ
LELAHLEHSLKIGVVQTAYANGSSTAYIKNTLHCPVSCTKTGVKHLHHEAATQYDIGI
YFEANGHGTIIFSEKFHRTIKSELSKSKLNGDTLALRTLKCFSELINQTVGDAISDML
AVLATLAILKMSPMDWDEEYTDLPNKLVKCIVPDRSIFQTTDQERKLLNPVGLQDKID
LVVAKYPMGRSFVRASGTEDAVRVYAECKDSSKLGQFCDEVVEHVKASA"
gene complement(<45020..>45721)
/gene="SDD1"
/locus_tag="YEL057C"
/db_xref="GeneID:856653"
mRNA complement(<45020..>45721)
/gene="SDD1"
/locus_tag="YEL057C"
/product="Sdd1p"
/transcript_id="NM_001178872.1"
/db_xref="GeneID:856653"
CDS complement(45020..45721)
/gene="SDD1"
/locus_tag="YEL057C"
/note="hypothetical protein; overproduction suppresses
lethality due to expression of the dominant PET9 allele
AAC2-A128P; may have a role in telomere maintenance;
target of UME6 regulation"
/codon_start=1
/product="Sdd1p"
/protein_id="NP_010857.1"
/db_xref="GeneID:856653"
/db_xref="SGD:S000000783"
/translation="MANDGIQRNDNRKGFKTVQFSAYSKEIDVIMKKISFLERNITQQ
LDTLPHFPKTLPPNHKDCVSRKHRARRGWSSQLKNLLGIYSKEEIFTLDNLAATLHDQ
VLKLQATLFPNAILKQVHLDNANIENKRILKEITYKYLSNENCKEENKFGTFIVKRIF
FGDLSLGVSVLINRIAFESATSSIMVVRSSFIESDFFYEDYLIFDCRAKRRKKLKRKI
LFISTTMNFNYQTKV"
gene <47168..>48373
/gene="HAT2"
/locus_tag="YEL056W"
/db_xref="GeneID:856654"
mRNA <47168..>48373
/gene="HAT2"
/locus_tag="YEL056W"
/product="Hat2p"
/transcript_id="NM_001178871.3"
/db_xref="GeneID:856654"
CDS 47168..48373
/gene="HAT2"
/locus_tag="YEL056W"
/experiment="EXISTENCE:direct assay:GO:0000123 histone
acetyltransferase complex [PMID:9575221|PMID:8858151]"
/experiment="EXISTENCE:direct assay:GO:0004402 histone
acetyltransferase activity [PMID:8858151]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14761951|PMID:9575221]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:8858151|PMID:9575221]"
/experiment="EXISTENCE:direct assay:GO:0006325 chromatin
organization [PMID:8858151]"
/experiment="EXISTENCE:direct assay:GO:0042393 histone
binding [PMID:8858151]"
/experiment="EXISTENCE:genetic interaction:GO:0031509
subtelomeric heterochromatin formation [PMID:10982821]"
/experiment="EXISTENCE:mutant phenotype:GO:0004402 histone
acetyltransferase activity [PMID:8858151|PMID:9575221]"
/experiment="EXISTENCE:physical interaction:GO:0000123
histone acetyltransferase complex [PMID:8858151]"
/note="Subunit of the Hat1p-Hat2p histone
acetyltransferase complex; required for high affinity
binding of the complex to free histone H4, thereby
enhancing Hat1p activity; similar to human RbAp46 and 48;
has a role in telomeric silencing"
/codon_start=1
/product="Hat2p"
/protein_id="NP_010858.3"
/db_xref="GeneID:856654"
/db_xref="SGD:S000000782"
/translation="MENQEKPLSVDEEYDLWKSNVPLMYDFVSETRLTWPSLTVQWLP
TPVQELDGGFIKQELIIGTHTSGEEENYLKFAEINLPKEILSNEDPQEEAGEEYQSSL
PAPRSNIRITAKYEHEEEITRARYMPQDPNIVATINGQGTVFLYSRSEGLQSTLKFHK
DNGYALSFSTLVKGRLLSGSDDHTVALWEVGSGGDPTKPVRTWNDLHSDIINDNKWHN
FNKDLFGTVSEDSLLKINDVRANNTTIDTVKCPQPFNTLAFSHHSSNLLAAAGMDSYV
YLYDLRNMKEPLHHMSGHEDAVNNLEFSTHVDGVVVSSGSDNRLMMWDLKQIGAEQTP
DDAEDGVPELIMVHAGHRSSVNDFDLNPQIPWLVASAEEENILQVWKCSHSLPIVGGP
PKVNKDIIS"
gene complement(<48471..>51539)
/gene="POL5"
/locus_tag="YEL055C"
/db_xref="GeneID:856655"
mRNA complement(<48471..>51539)
/gene="POL5"
/locus_tag="YEL055C"
/product="DNA-directed DNA polymerase"
/transcript_id="NM_001178870.1"
/db_xref="GeneID:856655"
CDS complement(48471..51539)
/gene="POL5"
/locus_tag="YEL055C"
/EC_number="2.7.7.7"
/experiment="EXISTENCE:direct assay:GO:0000027 ribosomal
large subunit assembly [PMID:31745560]"
/experiment="EXISTENCE:direct assay:GO:0000182 rDNA
binding [PMID:12093911]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:12093911|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0006364 rRNA
processing [PMID:31745560]"
/experiment="EXISTENCE:direct assay:GO:0042134 rRNA
primary transcript binding [PMID:31413149|PMID:31745560]"
/experiment="EXISTENCE:direct assay:GO:0090070 positive
regulation of ribosome biogenesis [PMID:31745560]"
/experiment="EXISTENCE:mutant phenotype:GO:0009303 rRNA
transcription [PMID:12093911]"
/experiment="EXISTENCE:mutant phenotype:GO:0090070
positive regulation of ribosome biogenesis
[PMID:31413149]"
/note="Protein involved in 60S assembly and pre-40S AFs
recycling; has sequence similarity to human MybBP1A and
weak sequence similarity to B-type DNA polymerases; not
required for chromosomal DNA replication"
/codon_start=1
/product="DNA-directed DNA polymerase"
/protein_id="NP_010859.1"
/db_xref="GeneID:856655"
/db_xref="SGD:S000000781"
/translation="MTGKVNRDLFFKLASDLREERLHAAVALIKDLSALDLPDDAEEW
SYVLNRLIKGLSSDRNSARLGFSLCLTEVINLAVNMPPGQRPKGLESTNEFLSTLSTI
LNVNVNEGTKKSMKGKDERGILFGKLFGLKSLLNEPLFSEIFVKDLEKGNTEFFIRFT
EQLIDLALKKNWIKEPCFFTLFQTMKMLLPFMDESSAEKILLIYDKYDLTLTNEGLST
YLLLKYEGDESLIPSVLDLKNPGWKDNDPLARGNLPLLTKVLRNSSVIPDANGGLKET
KKQKNTNWNPRLHFVWSVLLPLFGNGKLENTSHISKKRKKTNNKKVQNSIQFPEFWKM
AVDESFFNEKASSERKYLGFLIIDAAFKAVPGSYIGFCFSQNVMRTLINQSIDSQRVL
NKISQLTLDSIVKACEEDSANRLVPCLNAMLFGPHGSINFDKLTKSGTVSKLIAIKEL
PSTVLAQLLDVFFLQLQDKKGVLSHTLFALDSILHIVRAHKVEINDMDIMKPVLRPIV
YMAFFKHTSDDLKLEQLHELAKERLYSILGELTINKEIRCKDPEINSWQYLTLKLILD
IENSHVGDLINPLDENLENIKNEAISCLSKVCRSRTAQSWGLSTLLSMCLVQLYAGDT
DSISVIEELCEFSKHENNSMVGITEILLSLLAQKKALLRKLSLIIWQQFIEEVGLEEL
QILLDILKARENKQGFAQLFEGEEEFEEIKEENDASEDESKTGSESESESESDSDDAD
EKDEEDEANEDILNIDKEATSALVKALNLPDNIVNDKGEVDLDQLEGLSDDGGDDEDE
ESMDDEKMMELDDQLSEIFKRRKEALSSISTGNQRKFEVKQSRENVISFKHRVVDMLA
VYVKYCEKLTLANKSEHSNNLGGSLSKLVYFIIPMLKCVNETLDRPLADKISKLLKGK
IFKIKVTAFKDMNKDIELMDLLKKTHKLMLTSKPGQHAAVFYSMCSTSSLFLSKLYVE
IGGNDKLDELIDLYTATTKEWMQKGKCGPNIFIDFINWLSSKKQTVMDKE"
gene complement(52150..52320)
/gene="SNR80"
/locus_tag="YNCE0001C"
/db_xref="GeneID:9164883"
ncRNA complement(52150..52320)
/ncRNA_class="snoRNA"
/gene="SNR80"
/locus_tag="YNCE0001C"
/product="SNR80"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:15306656]"
/experiment="EXISTENCE:mutant phenotype:GO:0030559 rRNA
pseudouridylation guide activity
[PMID:15923376|PMID:15306656]"
/experiment="EXISTENCE:mutant phenotype:GO:0031118 rRNA
pseudouridine synthesis [PMID:15306656|PMID:15923376]"
/experiment="EXISTENCE:physical interaction:GO:0031429 box
H/ACA snoRNP complex [PMID:15923376]"
/note="H/ACA box small nucleolar RNA (snoRNA); guides
pseudouridylation of large subunit (LSU) rRNA at position
U776 and small subunit (SSU) rRNA at position U759"
/transcript_id="NR_132163.1"
/db_xref="GeneID:9164883"
/db_xref="SGD:S000081374"
gene complement(<52721..>53218)
/gene="RPL12A"
/locus_tag="YEL054C"
/db_xref="GeneID:856656"
mRNA complement(<52721..>53218)
/gene="RPL12A"
/locus_tag="YEL054C"
/product="60S ribosomal protein uL11 RPL12A"
/transcript_id="NM_001178869.1"
/db_xref="GeneID:856656"
CDS complement(52721..53218)
/gene="RPL12A"
/locus_tag="YEL054C"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:11983894]"
/experiment="EXISTENCE:curator inference:GO:0003735
structural constituent of ribosome [PMID:11983894]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:11983894]"
/experiment="EXISTENCE:genetic interaction:GO:0034517
ribophagy [PMID:39934334]"
/experiment="EXISTENCE:mutant phenotype:GO:0000027
ribosomal large subunit assembly [PMID:9822666]"
/note="Ribosomal 60S subunit protein L12A functions as a
ribophagy receptor; phosphorylation by Atg1p triggers
ribophagy during starvation; rpl12a rpl12b double mutant
exhibits slow growth and slow translation; homologous to
human RPL12, Drosophila rpl12, and C. elegans ppl-12 that
all function as conserved ribophagy receptors; RPL12A has
a paralog, RPL12B, that arose from the whole genome
duplication"
/codon_start=1
/product="60S ribosomal protein uL11 RPL12A"
/protein_id="NP_010860.1"
/db_xref="GeneID:856656"
/db_xref="SGD:S000000780"
/translation="MPPKFDPNEVKYLYLRAVGGEVGASAALAPKIGPLGLSPKKVGE
DIAKATKEFKGIKVTVQLKIQNRQAAASVVPSASSLVITALKEPPRDRKKDKNVKHSG
NIQLDEIIEIARQMRDKSFGRTLASVTKEILGTAQSVGCRVDFKNPHDIIEGINAGEI
EIPEN"
gene complement(<53901..>56102)
/gene="MAK10"
/locus_tag="YEL053C"
/gene_synonym="NAA35"
/db_xref="GeneID:856657"
mRNA complement(<53901..>56102)
/gene="MAK10"
/locus_tag="YEL053C"
/gene_synonym="NAA35"
/product="Mak10p"
/transcript_id="NM_001178868.3"
/db_xref="GeneID:856657"
CDS complement(53901..56102)
/gene="MAK10"
/locus_tag="YEL053C"
/gene_synonym="NAA35"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0031417 NatC
complex [PMID:10504710]"
/experiment="EXISTENCE:mutant phenotype:GO:0004596
protein-N-terminal amino-acid acetyltransferase activity
[PMID:11274203]"
/experiment="EXISTENCE:mutant phenotype:GO:0006474
N-terminal protein amino acid acetylation [PMID:11274203]"
/experiment="EXISTENCE:mutant phenotype:GO:0016236
macroautophagy [PMID:19793921]"
/note="Non-catalytic subunit of the NatC N-terminal
acetyltransferase; required for replication of dsRNA
virus; expression is glucose-repressible; human NatC
ortholog, Naa35, requires co-expression of the human
catalytic subunit, Naa30, to functionally complement the
null allele"
/codon_start=1
/product="Mak10p"
/protein_id="NP_010861.3"
/db_xref="GeneID:856657"
/db_xref="SGD:S000000779"
/translation="MEVDSILGSLSITDDFDQLVDVTSLFDELCSKLKPEAIVKDPRF
DLFEGTHSLEVNNSKLDSSLIELTAEEIEFDVNVAYDPPLASVAAIADRLLRCVISWL
NDYQTLPTTVLSCRYTESLLSSLVKGTTAGSSWCTGNILYDKVLGSCILGVCYLTKFV
QKLLSAGIVFEEEDLNFNNMGFNTFDNLPGQDVVINSLTESLQILEAYSDDSLHLTML
KHILKIIICLVHLEDHLTDYSTKTSHLDELIENANSVNGIFPQLQLSPPKGAFSTYIQ
KHRSNQFPPRKITKLPTDYSGFITLANDVKTILLVDKAESALETYQFAKFFNKLEQRH
VIARILFPLFFIRDDRTVLGKFSYTQFYLLHVKEFSAQTPSEFESSIGNELIQESSNM
LLEWYQNCSQNTCRYRQGFNRQLILWDSLQAQFESVNSQVYCSWTYFMKLSSMIEFSL
KGFDLDIYKPFEAYSMFWYVYYLSHHLETFLKDSQNDIESNINAIHSMNKKLKKLKAG
EKKDQLRLKYRFAMDNEMEQLQATKQFLNYLLKEINITKSLCLIEVFQFAILKSFGLI
DNKNSTPSKFSNERLIHNLRFKPFNSIGVPELPEYEVFQQTLKDFVIEEKGAAFDIKL
ERATNFIETEVRNVVSSIDEIMQGIKGGDNNGVLVTGTRLVQELSLEYYCKLKHTSKA
LSVNSKVIVNTLKKNIKNKDSHEYKVELVHTTEGWNYFPIQTLRIKQDRYK"
gene <56571..>58100
/gene="AFG1"
/locus_tag="YEL052W"
/db_xref="GeneID:856658"
mRNA <56571..>58100
/gene="AFG1"
/locus_tag="YEL052W"
/product="Afg1p"
/transcript_id="NM_001178867.1"
/db_xref="GeneID:856658"
CDS 56571..58100
/gene="AFG1"
/locus_tag="YEL052W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:14576278|PMID:16823961|PMID:14562095|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:17430883]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress [PMID:17430883]"
/experiment="EXISTENCE:mutant phenotype:GO:0141164
mitochondrial protein quality control [PMID:17430883]"
/note="Protein that may act as a chaperone for cytochrome
c oxidase subunits; conserved protein; may act as a
chaperone in the degradation of misfolded or unassembled
cytochrome c oxidase subunits; localized to matrix face of
the mitochondrial inner membrane; member of the AAA family
but lacks a protease domain"
/codon_start=1
/product="Afg1p"
/protein_id="NP_010862.1"
/db_xref="GeneID:856658"
/db_xref="SGD:S000000778"
/translation="MIALKPNAVRTFRQVQHCSFRICRYQSTKSNKCLTPLQEYDRLV
KLGKLRDDTYQRGIISSLGDLYDSLVKYVPPVVKTPNAVDQVGGWLNGLKSVFSRGKP
KNIGAYVDVSKIGNSIPRGVYLYGDVGCGKTMLMDLFYTTIPNHLTKKRIHFHQFMQY
VHKRSHEIVREQNLKELGDAKGKEIDTVPFLAAEIANNSHVLCFDEFQVTDVADAMIL
RRLMTALLSDDYGVVLFATSNRHPDELYINGVQRQSFIPCIELIKHRTKVIFLNSPTD
YRKIPRPVSSVYYFPSDTSIKYASKECKTRRETHIKEWYNYFAQASHTDDSTDSHTVH
KTFYDYPLTIWGREFKVPKCTPPRVAQFTFKQLCGEPLAAGDYLTLAKNFEAFIVTDI
PYLSIYVRDEVRRFITFLDAVYDSGGKLATTGAADFSSLFVEPEQILNDFELRPTTKE
PDSVDTGMVDEMVEKHGFSKEIAKKSQMFALDEERFAFARALSRLSQMSSTDWVTKPT
Y"
gene <58378..>59148
/gene="VMA8"
/locus_tag="YEL051W"
/db_xref="GeneID:856659"
mRNA <58378..>59148
/gene="VMA8"
/locus_tag="YEL051W"
/product="H(+)-transporting V1 sector ATPase subunit D"
/transcript_id="NM_001178866.1"
/db_xref="GeneID:856659"
CDS 58378..59148
/gene="VMA8"
/locus_tag="YEL051W"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:23836928|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0016471 vacuolar
proton-transporting V-type ATPase complex [PMID:10801866]"
/experiment="EXISTENCE:direct assay:GO:0045121 membrane
raft [PMID:23836928]"
/experiment="EXISTENCE:mutant phenotype:GO:0007035
vacuolar acidification [PMID:29519938]"
/experiment="EXISTENCE:mutant phenotype:GO:0046961
proton-transporting ATPase activity, rotational mechanism
[PMID:10801866]"
/note="Subunit D of the V1 peripheral membrane domain of
V-ATPase; part of the electrogenic proton pump found
throughout the endomembrane system; plays a role in the
coupling of proton transport and ATP hydrolysis; the V1
peripheral membrane domain of the vacuolar H+-ATPase
(V-ATPase) has eight subunits"
/codon_start=1
/product="H(+)-transporting V1 sector ATPase subunit D"
/protein_id="NP_010863.1"
/db_xref="GeneID:856659"
/db_xref="SGD:S000000777"
/translation="MSGNREQVFPTRMTLGLMKTKLKGANQGYSLLKRKSEALTKRFR
DITKRIDDAKQKMGRVMQTAAFSLAEVSYATGENIGYQVQESVSTARFKVRARQENVS
GVYLSQFESYIDPEINDFRLTGLGRGGQQVQRAKEIYSRAVETLVELASLQTAFIILD
EVIKVTNRRVNAIEHVIIPRTENTIAYINSELDELDREEFYRLKKVQEKKQNETAKLD
AEMKLKRDRAEQDASEVAADEEPQGETLVADQEDDVIF"
rep_origin 59338..59477
/note="ARS507; Autonomously Replicating Sequence"
/db_xref="SGD:S000077380"
gene complement(<59670..>60851)
/gene="RML2"
/locus_tag="YEL050C"
/gene_synonym="uL2m"
/db_xref="GeneID:856660"
mRNA complement(<59670..>60851)
/gene="RML2"
/locus_tag="YEL050C"
/gene_synonym="uL2m"
/product="mitochondrial 54S ribosomal protein uL2m RML2"
/transcript_id="NM_001178865.3"
/db_xref="GeneID:856660"
CDS complement(59670..60851)
/gene="RML2"
/locus_tag="YEL050C"
/gene_synonym="uL2m"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:9445368]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005762
mitochondrial large ribosomal subunit [PMID:9445368]"
/experiment="EXISTENCE:mutant phenotype:GO:0032543
mitochondrial translation [PMID:9079633]"
/note="Mitochondrial ribosomal protein of the large
subunit (L2); has similarity to E. coli L2 ribosomal
protein; mutant allele (fat21) causes inability to utilize
oleate, and induce oleic acid oxidation; may interfere
with activity of the Adr1p transcription factor"
/codon_start=1
/product="mitochondrial 54S ribosomal protein uL2m RML2"
/protein_id="NP_010864.3"
/db_xref="GeneID:856660"
/db_xref="SGD:S000000776"
/translation="MLVLGSLRSALSCSSTASLISKRNPCYPYGILCRTLSQSVKLWQ
ENTSKDDSSLNITPRLLKIIPNDTDIVTLEKQDELIKRRRKLSKEVTQMKRLKPVSPG
LRWYRSPIYPYLYKGRPVRALTVVRKKHGGRNNSGKITVRHQGGGHRNRTRLIDFNRW
EGGAQTVQRIEYDPGRSSHIALLKHNTTGELSYIIACDGLRPGDVVESFRRGIPQTLL
NEMGGKVDPAILSVKTTQRGNCLPISMIPIGTIIHNVGITPVGPGKFCRSAGTYARVL
AKLPEKKKAIVRLQSGEHRYVSLEAVATIGVVSNIDHQNRSLGKAGRSRWLGIRPTVR
GVAMNKCDHPHGGGRGKSKSNKLSMSPWGQLAKGYKTRRGKNQNRMKVKDRPRGKDAR
L"
gene 61352..61433
/gene="SNR67"
/locus_tag="YNCE0002W"
/db_xref="GeneID:9164880"
ncRNA 61352..61433
/ncRNA_class="snoRNA"
/gene="SNR67"
/locus_tag="YNCE0002W"
/product="SNR67"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:10024243]"
/experiment="EXISTENCE:curator inference:GO:0031428 box
C/D methylation guide snoRNP complex [PMID:10024243]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:31804585]"
/experiment="EXISTENCE:mutant phenotype:GO:0030562 rRNA
2'-O-ribose methylation guide activity [PMID:10024243]"
/experiment="EXISTENCE:mutant phenotype:GO:0031167 rRNA
methylation [PMID:10024243]"
/note="C/D box small nucleolar RNA (snoRNA); guides
2'-O-methylation of large subunit (LSU) rRNA at positions
G2619 and U2724"
/transcript_id="NR_132164.1"
/db_xref="GeneID:9164880"
/db_xref="SGD:S000006458"
gene 61699..61789
/gene="SNR53"
/locus_tag="YNCE0003W"
/db_xref="GeneID:9164881"
ncRNA 61699..61789
/ncRNA_class="snoRNA"
/gene="SNR53"
/locus_tag="YNCE0003W"
/product="SNR53"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:10024243]"
/experiment="EXISTENCE:curator inference:GO:0031428 box
C/D methylation guide snoRNP complex [PMID:10024243]"
/experiment="EXISTENCE:mutant phenotype:GO:0030562 rRNA
2'-O-ribose methylation guide activity [PMID:10024243]"
/experiment="EXISTENCE:mutant phenotype:GO:0031167 rRNA
methylation [PMID:10024243]"
/note="C/D box small nucleolar RNA (snoRNA); guides
2'-O-methylation of small subunit (SSU) rRNA at position
A796"
/transcript_id="NR_132165.1"
/db_xref="GeneID:9164881"
/db_xref="SGD:S000006444"
gene complement(61890..61960)
/locus_tag="YNCE0004C"
/db_xref="GeneID:856661"
tRNA complement(61890..61960)
/locus_tag="YNCE0004C"
/product="tRNA-Gly"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0030371 translation
repressor activity [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043555 regulation
of translation in response to stress [PMID:27609601]"
/note="Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:856661"
/db_xref="SGD:S000006578"
repeat_region 62081..62756
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006889"
repeat_region 62142..62474
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006890"
gene <63728..>64090
/gene="PAU2"
/locus_tag="YEL049W"
/db_xref="GeneID:856662"
mRNA <63728..>64090
/gene="PAU2"
/locus_tag="YEL049W"
/product="seripauperin PAU2"
/transcript_id="NM_001178864.1"
/db_xref="GeneID:856662"
CDS 63728..64090
/gene="PAU2"
/locus_tag="YEL049W"
/note="Member of the seripauperin multigene family;
encoded mainly in subtelomeric region; active during
alcoholic fermentation; regulated by anaerobiosis;
negatively regulated by oxygen; repressed by heme"
/codon_start=1
/product="seripauperin PAU2"
/protein_id="NP_010865.1"
/db_xref="GeneID:856662"
/db_xref="SGD:S000000775"
/translation="MVKLTSIAAGVAAIAATASATTTLAQSDERVNLVELGVYVSDIR
AHLAQYYSFQAAHPTETYPIEVAEAVFNYGDFTTMLTGIAPDQVTRMITGVPWYSSRL
KPAISSALSKDGIYTIAN"
gene complement(<64709..>65167)
/gene="TCA17"
/locus_tag="YEL048C"
/db_xref="GeneID:856663"
mRNA complement(<64709..>65167)
/gene="TCA17"
/locus_tag="YEL048C"
/product="Tca17p"
/transcript_id="NM_001178863.1"
/db_xref="GeneID:856663"
CDS complement(64709..65167)
/gene="TCA17"
/locus_tag="YEL048C"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:19220810]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:1990071 TRAPPII
protein complex [PMID:21453443]"
/experiment="EXISTENCE:mutant phenotype:GO:0034498 early
endosome to Golgi transport [PMID:19220810]"
/experiment="EXISTENCE:mutant phenotype:GO:0065003
protein-containing complex assembly [PMID:21453443]"
/note="Component of transport protein particle (TRAPP)
complex II; TRAPPII is a multimeric guanine
nucleotide-exchange factor for the GTPase Ypt1p,
regulating intra-Golgi and endosome-Golgi traffic;
promotes association of TRAPPII-specific subunits with the
TRAPP core complex; sedlin related; human Sedlin mutations
cause SEDT, a skeletal disorder"
/codon_start=1
/product="Tca17p"
/protein_id="NP_010866.1"
/db_xref="GeneID:856663"
/db_xref="SGD:S000000774"
/translation="MSLRPCFVSLIDESDKPILIYVPNEAENEMNDVLKYNVLSNISL
DYFESALVEWHSLDSKPLLKSIFQLEGVSVFAMLIKQTGLKIVIGFEQKSLSGADDEF
EAINQIFETVRKIYIRVKCNPLLVSGDEKSIIKSLERKFDELFISTEVEL"
gene complement(<65385..>66797)
/gene="FRD1"
/locus_tag="YEL047C"
/db_xref="GeneID:856664"
mRNA complement(<65385..>66797)
/gene="FRD1"
/locus_tag="YEL047C"
/product="fumarate reductase"
/transcript_id="NM_001178862.3"
/db_xref="GeneID:856664"
CDS complement(65385..66797)
/gene="FRD1"
/locus_tag="YEL047C"
/EC_number="1.3.1.6"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:9587404]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836]"
/experiment="EXISTENCE:direct assay:GO:0016156 fumarate
reductase (NADH) activity [PMID:8946166]"
/experiment="EXISTENCE:genetic interaction:GO:0071454
cellular response to anoxia [PMID:9711846|PMID:17345583]"
/note="Soluble fumarate reductase; required with isoenzyme
Osm1p for anaerobic growth; may interact with ribosomes,
based on co-purification experiments; authentic,
non-tagged protein is detected in purified mitochondria in
high-throughput studies; similar to Arxula adeninovorans
fumarate reductase; protein abundance increases in
response to DNA replication stress; FRD1 has a paralog,
OSM1, that arose from the whole genome duplication"
/codon_start=1
/product="fumarate reductase"
/protein_id="NP_010867.3"
/db_xref="GeneID:856664"
/db_xref="SGD:S000000773"
/translation="MSLSPVVVIGTGLAGLAAANELVNKYNIPVTILEKASSIGGNSI
KASSGINGACTETQRHFHIEDSPRLFEDDTIKSAKGKGVQELMAKLANDSPLAIEWLK
NEFDLKLDLLAQLGGHSVARTHRSSGKLPPGFEIVSALSNNLKKLAETKPELVKINLD
SKVVDIHEKDGSISAVVYEDKNGEKHMVSANDVVFCSGGFGFSKEMLKEYAPELVNLP
TTNGQQTTGDGQRLLQKLGADLIDMDQIQVHPTGFIDPNDRSSSWKFLAAESLRGLGG
ILLNPITGRRFVNELTTRDVVTAAIQKVCPQEDNRALLVMGEKMYTDLKNNLDFYMFK
KLVQKLTLSQVVSEYNLPITVAQLCEELQTYSSFTTKADPLGRTVILNEFGSDVTPET
VVFIGEVTPVVHFTMGGARINVKAQVIGKNDERLLKGLYAAGEVSGGVHGANRLGGSS
LLECVVFGRTAAESIANDRK"
gene complement(<67629..>68792)
/gene="GLY1"
/locus_tag="YEL046C"
/db_xref="GeneID:856665"
mRNA complement(<67629..>68792)
/gene="GLY1"
/locus_tag="YEL046C"
/product="threonine aldolase GLY1"
/transcript_id="NM_001178861.1"
/db_xref="GeneID:856665"
CDS complement(67629..68792)
/gene="GLY1"
/locus_tag="YEL046C"
/EC_number="4.1.2.48"
/experiment="EXISTENCE:direct assay:GO:0004793 threonine
aldolase activity [PMID:9163906]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:12676688]"
/experiment="EXISTENCE:direct assay:GO:0006545 glycine
biosynthetic process [PMID:3086094]"
/experiment="EXISTENCE:direct assay:GO:0006567 L-threonine
catabolic process [PMID:3086094]"
/experiment="EXISTENCE:direct assay:GO:0008732
L-allo-threonine aldolase activity [PMID:9151955]"
/note="Threonine aldolase; catalyzes the cleavage of
L-allo-threonine and L-threonine to glycine; involved in
glycine biosynthesis"
/codon_start=1
/product="threonine aldolase GLY1"
/protein_id="NP_010868.1"
/db_xref="GeneID:856665"
/db_xref="SGD:S000000772"
/translation="MTEFELPPKYITAANDLRSDTFTTPTAEMMEAALEASIGDAVYG
EDVDTVRLEQTVARMAGKEAGLFCVSGTLSNQIAIRTHLMQPPYSILCDYRAHVYTHE
AAGLAILSQAMVVPVVPSNGDYLTLEDIKSHYVPDDGDIHGAPTRLISLENTLHGIVY
PLEELVRIKAWCMENGLKLHCDGARIWNAAAQSGVPLKQYGEIFDSISICLSKSMGAP
IGSVLVGNLKFVKKATHFRKQQGGGIRQSGMMARMALVNINNDWKSQLLYSHSLAHEL
AEYCEAKGIPLESPADTNFVFINLKAARMDPDVLVKKGLKYNVKLMGGRVSFHYQVTR
DTLEKVKLAISEAFDYAKEHPFDCNGPTQIYRSESTEVDVDGNAIREIKTYKY"
gene <69757..>70257
/gene="IES6"
/locus_tag="YEL044W"
/db_xref="GeneID:856667"
mRNA <69757..>70257
/gene="IES6"
/locus_tag="YEL044W"
/product="Ies6p"
/transcript_id="NM_001178859.1"
/db_xref="GeneID:856667"
CDS 69757..70257
/gene="IES6"
/locus_tag="YEL044W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:physical interaction:GO:0031011
Ino80 complex [PMID:24034245]"
/note="Component of the INO80 chromatin remodeling
complex; critical for INO80 function; involved in
regulation of chromosome segregation and maintenance of
normal centromeric chromatin structure; human ortholog
INO80C is a member of the human INO80 complex; implicated
in DNA repair based on genetic interactions with RAD52
epistasis genes"
/codon_start=1
/product="Ies6p"
/protein_id="NP_010870.1"
/db_xref="GeneID:856667"
/db_xref="SGD:S000000770"
/translation="MSGSRGNSSNSSVSNNSNNNNNNDGGDERLLFLRSVGERNEIGF
PSRFKSAHYKKPTRRHKSARQLISDENKRINALLTKANKAAESSTAARRLVPKATYFS
VEAPPSIRPAKKYCDVTGLKGFYKSPTNNIRYHNAEIYQLIVKPMAPGVDQEYLKLRG
ANFVLK"
gene <70478..>73348
/gene="GTA1"
/locus_tag="YEL043W"
/db_xref="GeneID:856668"
mRNA <70478..>73348
/gene="GTA1"
/locus_tag="YEL043W"
/product="Gta1p"
/transcript_id="NM_001178858.3"
/db_xref="GeneID:856668"
CDS 70478..73348
/gene="GTA1"
/locus_tag="YEL043W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="hypothetical protein; proposed role in Golgi
vesicle trafficking based on phenotypic profile, presence
of a coiled-coil domain often associated with vesicle
tethering proteins, and interaction profile process
enrichment; may interact with ribosomes, based on
co-purification studies; localizes to the ER and cell
periphery in HTP studies; contains fibronectin type III
domain fold"
/codon_start=1
/product="Gta1p"
/protein_id="NP_010871.3"
/db_xref="GeneID:856668"
/db_xref="SGD:S000000769"
/translation="MPVSVITTVLACLWLSYRLYKFLTIPVSSIVSTLKIKTPPATKV
SIDKIATDSVTIHWENEPVKAEDNGSADRNFISHYLLYLNNTQLAIFPNNPNSLYTCC
SITGLEAETQYQLDFITINNKGFINKLPSIYCMTKAREANEALKTRKWRRNTITSSTA
MQPRNSKSEPAPLPSHYSSVSLSTFSSNITNSATSNNGSNLPAYTSLTTLKDLDSFSI
DDLKKILICAQEDLHDVLSQQTSLLQDFQESKLELELELDNLKTHWSHEIDLRKSLKS
NIKSLENSKLLTDLKIEKLNKKIDKSKEKISKMRNDMQKWSQEDTELLSKDTIKEKYF
KLLNESNASVANINKEIESLQNEISKMEESNKRLNASKKSLITSIVVNANVENDKPIA
SGELSAVLKKLNDFTLEKNGFLSNAGEEFLSKLNADSSLIKMIKQELSIDQELEANWK
LQRSNLLKKISALENQFNEMSLNNRNLKTKLMVQPYKNNGDSLAATNSNNSAEKNRSS
GSIQLPLSNNMSRTGSIDLISNNNKSINNSNADSAPPLRLHNPVSYSPSNEPIQPSSS
LLSQLTQDTDNRSMLSNHISSNNENKQQPSSYSHALPTTATANATATATATNGHSRSN
LWTTAQFAQPSHQQVSTELDQAFEYDNANHLISGLQNMIYDETDYPDNISNYSKGFTT
DELDNYWTKQQPQVRSTNESLFSTTGTPMSSYKANPVISPYSSSHLRQTSNATNTNPM
HPQSLLAATLNDPSLQSFVRSGSFYSAPQPANSLQNNINGNETENISPRISSDFNLLV
PNLSPRLSNDVPIVPGNNTTLTPSHSNILTMNHQPTADNITRRSFHASSPPFNSIWNS
NTNQLSPPLEEQYHLDVPVGPKVPAKEPSPKPSHKRNQSNSSISSAWSKFKHKSASSP
ANADTDIQDSSTPSTSPSGRRMSKLLSKSGMNNLFNPHSHDS"
gene <73771..>75327
/gene="GDA1"
/locus_tag="YEL042W"
/db_xref="GeneID:856669"
mRNA <73771..>75327
/gene="GDA1"
/locus_tag="YEL042W"
/product="guanosine diphosphatase"
/transcript_id="NM_001178857.1"
/db_xref="GeneID:856669"
CDS 73771..75327
/gene="GDA1"
/locus_tag="YEL042W"
/EC_number="3.6.1.42"
/experiment="EXISTENCE:direct assay:GO:0004382 GDP
phosphatase activity [PMID:7506254]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:9614179]"
/experiment="EXISTENCE:direct assay:GO:0045134 UDP
phosphatase activity [PMID:11425802]"
/experiment="EXISTENCE:mutant phenotype:GO:0009101
glycoprotein biosynthetic process [PMID:8391537]"
/note="Guanosine diphosphatase located in the Golgi;
involved in the transport of GDP-mannose into the Golgi
lumen, converting GDP to GMP after mannose is transferred
to substrates; null mutants are defective in sporulation
and pre-meiotic S phase entry; orthologous to human
ENTPD6, a meiosis-associated non-obstructive azoospermia
(NOA) related gene identified in GWAS studies"
/codon_start=1
/product="guanosine diphosphatase"
/protein_id="NP_010872.1"
/db_xref="GeneID:856669"
/db_xref="SGD:S000000768"
/translation="MAPIFRNYRFAIGAFAVIMLILLIKTSSIGPPSIARTVTPNASI
PKTPEDISILPVNDEPGYLQDSKTEQNYPELADAVKSQTSQTCSEEHKYVIMIDAGST
GSRVHIYKFDVCTSPPTLLDEKFDMLEPGLSSFDTDSVGAANSLDPLLKVAMNYVPIK
ARSCTPVAVKATAGLRLLGDAKSSKILSAVRDHLEKDYPFPVVEGDGVSIMGGDEEGV
FAWITTNYLLGNIGANGPKLPTAAVFDLGGGSTQIVFEPTFPINEKMVDGEHKFDLKF
GDENYTLYQFSHLGYGLKEGRNKVNSVLVENALKDGKILKGDNTKTHQLSSPCLPPKV
NATNEKVTLESKETYTIDFIGPDEPSGAQCRFLTDEILNKDAQCQSPPCSFNGVHQPS
LVRTFKESNDIYIFSYFYDRTRPLGMPLSFTLNELNDLARIVCKGEETWNSVFSGIAG
SLDELESDSHFCLDLSFQVSLLHTGYDIPLQRELRTGKKIANKEIGWCLGASLPLLKA
DNWKCKIQSA"
gene <75944..>77431
/gene="YEF1"
/locus_tag="YEL041W"
/db_xref="GeneID:856670"
mRNA <75944..>77431
/gene="YEF1"
/locus_tag="YEL041W"
/product="NADH/NAD(+) kinase"
/transcript_id="NM_001178856.1"
/db_xref="GeneID:856670"
CDS 75944..77431
/gene="YEF1"
/locus_tag="YEL041W"
/EC_number="2.7.1.86"
/experiment="EXISTENCE:direct assay:GO:0003951 NAD+ kinase
activity [PMID:15978040]"
/experiment="EXISTENCE:direct assay:GO:0006741 NADP+
biosynthetic process [PMID:15978040]"
/experiment="EXISTENCE:direct assay:GO:0042736 NADH kinase
activity [PMID:15978040]"
/note="ATP-NADH kinase; phosphorylates both NAD and NADH;
homooctameric structure consisting of 60-kDa subunits;
similar to Pos5p; overexpression complements certain pos5
phenotypes; YEF1 has a paralog, UTR1, that arose from the
whole genome duplication"
/codon_start=1
/product="NADH/NAD(+) kinase"
/protein_id="NP_010873.1"
/db_xref="GeneID:856670"
/db_xref="SGD:S000000767"
/translation="MKTDRLLINASPETCTKGDAEMDTMDTIDRMTSVKVLAEGKVLS
NFEEPGLMRCGYHDAKNWVRRLSSETIVGEDTSNLYPFYVDTAYDVRRLRKDLINAKV
DLQVENLIIICNINDISTVFLMREVVEWILRNFHSITVYVQDIFKKSTQFAVGDLCKD
SNCSKNRVKYWSKEFVKKHDSFFDLMITLGGDGTVLFASSIFTKDVPPIVPFALGSLG
FLTNFEFQNFKETLKHILTDEVRINLRMRLQCKLYRRNKPEIDAATGRKICYIDFISE
HHVLNEVTIDRGPAPCLSLLELYGNDSLMTKVQGDGLIVATPTGSTAYSLSAGGSLIS
PSVNAIAVTPICPHTLSFRPIILPDSMELKVRVDMNSRGTSWVNFDGKDRVELKQGDY
VVITASPYSVPTIESSASEFFESISKNLNWNDREEQKPFAHILSPKNQEKYRLDSSKN
GNDTISNPLESSCISSDAQDEERKSVTETETEIVVERTRQAHFAI"
gene <78053..>79456
/gene="UTR2"
/locus_tag="YEL040W"
/gene_synonym="CRH2"
/db_xref="GeneID:856671"
mRNA <78053..>79456
/gene="UTR2"
/locus_tag="YEL040W"
/gene_synonym="CRH2"
/product="chitin transglycosylase UTR2"
/transcript_id="NM_001178855.3"
/db_xref="GeneID:856671"
CDS 78053..79456
/gene="UTR2"
/locus_tag="YEL040W"
/gene_synonym="CRH2"
/EC_number="3.2.1.14"
/experiment="EXISTENCE:direct assay:GO:0000144 cellular
bud neck septin ring [PMID:12045225]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:10383953|PMID:15781460]"
/experiment="EXISTENCE:direct assay:GO:0016757
glycosyltransferase activity [PMID:23919454]"
/experiment="EXISTENCE:genetic interaction:GO:0006030
chitin metabolic process [PMID:18694928]"
/experiment="EXISTENCE:genetic interaction:GO:0016757
glycosyltransferase activity [PMID:18694928]"
/experiment="EXISTENCE:genetic interaction:GO:0031505
fungal-type cell wall organization [PMID:18694928]"
/experiment="EXISTENCE:mutant phenotype:GO:0006030 chitin
metabolic process [PMID:18694928]"
/experiment="EXISTENCE:mutant phenotype:GO:0016757
glycosyltransferase activity [PMID:18694928]"
/experiment="EXISTENCE:mutant phenotype:GO:0031505
fungal-type cell wall organization [PMID:18694928]"
/note="Chitin transglycosylase; functions in the transfer
of chitin to beta(1-6) and beta(1-3) glucans in the cell
wall; similar to and functionally redundant with Crh1;
glycosylphosphatidylinositol (GPI)-anchored protein
localized to bud neck"
/codon_start=1
/product="chitin transglycosylase UTR2"
/protein_id="NP_010874.3"
/db_xref="GeneID:856671"
/db_xref="SGD:S000000766"
/translation="MAIVNSWLICLVSIFSFVVRVEAATFCNATQACPEDKPCCSQYG
ECGTGQYCLNNCDVRYSFSHDSCMPVPICKSSSTKFKDYSSKLGNANTFLGNVSEADW
LYTGDVLDYDDEESLILAMPKNSGGTVLSSTRAVWYGKVSARIKTSHLAGVVTGFILY
SGAGDELDYEFVGADLETAQTNFYWESVLNYTNSANISTTDTFENYHTYELDWHEDYV
TWSIDGVVGRTLYKNETYNATTQKYQYPQTPSKVDISIWPGGNSTNAPGTIAWSGGEI
NWDASDISNPGYYYAIVNEVNITCYDPPSDTKKNGTSAYVYTSSSEFLAKDIAITDDE
VMMDSDEGSGLDPHKGATTSSTQKSSSSTATSSSKTSSDHSSSTKKSSKTSSTASSSS
SSSSSSSSSSSTATKNGDKVVSSVSSSVTSQTQTTSSVSGSASSSTSSMSGNNAGANV
AANWRLTVLCVILGYVL"
gene complement(<79636..>79977)
/gene="CYC7"
/locus_tag="YEL039C"
/db_xref="GeneID:856672"
mRNA complement(<79636..>79977)
/gene="CYC7"
/locus_tag="YEL039C"
/product="cytochrome c isoform 2"
/transcript_id="NM_001178854.1"
/db_xref="GeneID:856672"
CDS complement(79636..79977)
/gene="CYC7"
/locus_tag="YEL039C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005758
mitochondrial intermembrane space [PMID:8636074]"
/experiment="EXISTENCE:direct assay:GO:0006122
mitochondrial electron transport, ubiquinol to cytochrome
c [PMID:7814361]"
/experiment="EXISTENCE:direct assay:GO:0006123
mitochondrial electron transport, cytochrome c to oxygen
[PMID:7814361]"
/experiment="EXISTENCE:direct assay:GO:0009055 electron
transfer activity [PMID:7814361]"
/note="Cytochrome c isoform 2, expressed under hypoxic
conditions; also known as iso-2-cytochrome c; electron
carrier of the mitochondrial intermembrane space that
transfers electrons from ubiquinone-cytochrome c
oxidoreductase to cytochrome c oxidase during cellular
respiration; protein abundance increases in response to
DNA replication stress; CYC7 has a paralog, CYC1, that
arose from the whole genome duplication"
/codon_start=1
/product="cytochrome c isoform 2"
/protein_id="NP_010875.1"
/db_xref="GeneID:856672"
/db_xref="SGD:S000000765"
/translation="MAKESTGFKPGSAKKGATLFKTRCQQCHTIEEGGPNKVGPNLHG
IFGRHSGQVKGYSYTDANINKNVKWDEDSMSEYLTNPKKYIPGTKMAFAGLKKEKDRN
DLITYMTKAAK"
gene <80462..>81145
/gene="UTR4"
/locus_tag="YEL038W"
/db_xref="GeneID:856673"
mRNA <80462..>81145
/gene="UTR4"
/locus_tag="YEL038W"
/product="putative acireductone synthase UTR4"
/transcript_id="NM_001178853.1"
/db_xref="GeneID:856673"
CDS 80462..81145
/gene="UTR4"
/locus_tag="YEL038W"
/EC_number="3.1.3.77"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0019509
L-methionine salvage from methylthioadenosine
[PMID:18625006]"
/note="Protein with sequence similarity to acireductone
synthases; involved in methionine salvage; found in both
the cytoplasm and nucleus"
/codon_start=1
/product="putative acireductone synthase UTR4"
/protein_id="NP_010876.2"
/db_xref="GeneID:856673"
/db_xref="SGD:S000000764"
/translation="MGDNYSTYLLDIEGTVCPISFVKETLFPYFTNKVPQLVQQDTRD
SPVSNILSQFHIDNKEQLQAHILELVAKDVKDPILKQLQGYVWAHGYESGQIKAPVYA
DAIDFIKRKKRVFIYSSGSVKAQKLLFGYVQDPNAPAHDSLDLNSYIDGYFDINTSGK
KTETQSYANILRDIGAKASEVLFLSDNPLELDAAAGVGIATGLASRPGNAPVPDGQKY
QVYKNFETL"
gene complement(<81407..>82603)
/gene="RAD23"
/locus_tag="YEL037C"
/db_xref="GeneID:856674"
mRNA complement(<81407..>82603)
/gene="RAD23"
/locus_tag="YEL037C"
/product="Rad23p"
/transcript_id="NM_001178852.3"
/db_xref="GeneID:856674"
CDS complement(81407..82603)
/gene="RAD23"
/locus_tag="YEL037C"
/experiment="EXISTENCE:direct assay:GO:0000111
nucleotide-excision repair factor 2 complex
[PMID:7768886]"
/experiment="EXISTENCE:direct assay:GO:0000224
peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase
activity [PMID:20016784]"
/experiment="EXISTENCE:direct assay:GO:0003684 damaged DNA
binding [PMID:9837874|PMID:9813069]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0006517 protein
deglycosylation [PMID:20016784]"
/experiment="EXISTENCE:direct assay:GO:0036435 K48-linked
polyubiquitin modification-dependent protein binding
[PMID:28525741]"
/experiment="EXISTENCE:direct assay:GO:0043130 ubiquitin
binding [PMID:11323716]"
/experiment="EXISTENCE:direct assay:GO:0070628 proteasome
binding [PMID:15117949|PMID:15242647|PMID:25311859]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:26150418]"
/experiment="EXISTENCE:mutant phenotype:GO:0036503 ERAD
pathway [PMID:15167887]"
/experiment="EXISTENCE:mutant phenotype:GO:0043161
proteasome-mediated ubiquitin-dependent protein catabolic
process [PMID:19889839]"
/experiment="EXISTENCE:physical interaction:GO:0030674
protein-macromolecule adaptor activity [PMID:15121879]"
/note="Proteasome-associated ubiquitin receptor;
K48-specific Ub chain binding protein that recruits
substrates to the proteasome; subunit of nuclear excision
repair factor 2 (NER2) with Rad4p that binds damaged DNA
during NER, linking repair to proteolysis; NER2 binds DDR
gene promoters to repress transcription in the absence of
damage; stimulates Png1p-induced protein deglycosylation;
contains a Ub-like (UBL) and two Ub-associated (UBA)
domains; UBA2 protects Rad23p from proteasomal
degradation"
/codon_start=1
/product="Rad23p"
/protein_id="NP_010877.3"
/db_xref="GeneID:856674"
/db_xref="SGD:S000000763"
/translation="MVSLTFKNFKKEKVPLDLEPSNTILETKTKLAQSISCEESQIKL
IYSGKVLQDSKTVSECGLKDGDQVVFMVSQKKSTKTKVTEPPIAPESATTPGRENSTE
ASPSTDASAAPAATAPEGSQPQEEQTATTERTESASTPGFVVGTERNETIERIMEMGY
QREEVERALRAAFNNPDRAVEYLLMGIPENLRQPEPQQQTAAAAEQPSTAATTAEQPA
EDDLFAQAAQGGNASSGALGTTGGATDAAQGGPPGSIGLTVEDLLSLRQVVSGNPEAL
APLLENISARYPQLREHIMANPEVFVSMLLEAVGDNMQDVMEGADDMVEGEDIEVTGE
AAAAGLGQGEGEGSFQVDYTPEDDQAISRLCELGFERDLVIQVYFACDKNEEAAANIL
FSDHAD"
gene complement(<83050..>84552)
/gene="ANP1"
/locus_tag="YEL036C"
/gene_synonym="GEM3; MNN8"
/db_xref="GeneID:856675"
mRNA complement(<83050..>84552)
/gene="ANP1"
/locus_tag="YEL036C"
/gene_synonym="GEM3; MNN8"
/product="Anp1p"
/transcript_id="NM_001178851.1"
/db_xref="GeneID:856675"
CDS complement(83050..84552)
/gene="ANP1"
/locus_tag="YEL036C"
/gene_synonym="GEM3; MNN8"
/experiment="EXISTENCE:direct assay:GO:0000009
alpha-1,6-mannosyltransferase activity [PMID:10037752]"
/experiment="EXISTENCE:direct assay:GO:0000136 mannan
polymerase complex [PMID:9430634]"
/experiment="EXISTENCE:direct assay:GO:0000137 Golgi cis
cisterna [PMID:9430634]"
/experiment="EXISTENCE:direct assay:GO:0006487 protein
N-linked glycosylation [PMID:9430634]"
/experiment="EXISTENCE:mutant phenotype:GO:0006487 protein
N-linked glycosylation [PMID:9430634]"
/note="Subunit of the alpha-1,6 mannosyltransferase
complex; type II membrane protein; has a role in retention
of glycosyltransferases in the Golgi; involved in osmotic
sensitivity and resistance to aminonitrophenyl
propanediol"
/codon_start=1
/product="Anp1p"
/protein_id="NP_010878.1"
/db_xref="GeneID:856675"
/db_xref="SGD:S000000762"
/translation="MKYNNRKLSFNPTTVSIAGTLLTVFFLTRLVLSFFSISLFQLVT
FQGIFKPYVPDFKNTPSVEFYDLRNYQGNKDGWQQGDRILFCVPLRDASEHLPMFFNH
LNTMTYPHNLIDLSFLVSDSSDNTMGVLLSNLQMAQSQQDKSKRFGNIEIYEKDFGQI
IGQSFSDRHGFGAQGPRRKLMARARNWLGSVALKPYHSWVYWRDVDVETIPTTIMEDL
MHHDKDVIVPNVWRPLPDWLGNIQPYDLNSWKESEGGLQLADSLDEDAVIVEGYPEYA
TWRPHLAYMRDPNGNPEDEMELDGIGGVSILAKAKVFRTGSHFPAFSFEKHAETEAFG
RLSRRMNYNVIGLPHYVIWHIYEPSSDDLKHMAWMAEEEKRKLEEERIREFYNKIWEI
GFEDVRDQWNEERDSILKNIDSTLNNKVTVDWSEEGDGSELVDSKGDFVSPNNQQQQQ
QQQQQQQQQQQQQQQQQLDGNPQGKPLDDNDKNKKKHPKEVPLDFDPDRN"
gene complement(<85045..>85545)
/gene="UTR5"
/locus_tag="YEL035C"
/db_xref="GeneID:856676"
mRNA complement(<85045..>85545)
/gene="UTR5"
/locus_tag="YEL035C"
/product="Utr5p"
/transcript_id="NM_001178850.1"
/db_xref="GeneID:856676"
CDS complement(85045..85545)
/gene="UTR5"
/locus_tag="YEL035C"
/note="hypothetical protein; originally considered
essential but deletion overlapped the TATA box of
neighboring essential gene HYP2, while deletion that
avoids the HYP2 TATA box is viable; transcription may be
regulated by Gcr1p"
/codon_start=1
/product="Utr5p"
/protein_id="NP_010879.1"
/db_xref="GeneID:856676"
/db_xref="SGD:S000000761"
/translation="MSRYGKNLVHYIIVEHDDQRGQKPIDDDDEKNFYYHCSFTFETF
FRATAFLLAPAVCAVREVPCRLTRTRYNATEYIEGYGWMISLQQGLGVAEFYRPWPLS
VQLQRYTTPSRRSRFAVLTPQRKCHQNEANGQDLSLLILLSRIYPLCSNTSQTRRVAA
RKGKLS"
gene <85676..>86149
/gene="HYP2"
/locus_tag="YEL034W"
/gene_synonym="TIF51A"
/db_xref="GeneID:856677"
mRNA <85676..>86149
/gene="HYP2"
/locus_tag="YEL034W"
/gene_synonym="TIF51A"
/product="translation elongation factor eIF-5A"
/transcript_id="NM_001178849.3"
/db_xref="GeneID:856677"
CDS 85676..86149
/gene="HYP2"
/locus_tag="YEL034W"
/gene_synonym="TIF51A"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:19120453]"
/experiment="EXISTENCE:direct assay:GO:0003743 translation
initiation factor activity [PMID:21451136]"
/experiment="EXISTENCE:direct assay:GO:0003746 translation
elongation factor activity [PMID:19424157]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:16215987]"
/experiment="EXISTENCE:direct assay:GO:0045901 positive
regulation of translational elongation [PMID:19424157]"
/experiment="EXISTENCE:direct assay:GO:0045905 positive
regulation of translational termination [PMID:19424157]"
/experiment="EXISTENCE:direct assay:GO:0045948 positive
regulation of translational initiation [PMID:21451136]"
/experiment="EXISTENCE:direct assay:GO:0048471 perinuclear
region of cytoplasm [PMID:11861547]"
/experiment="EXISTENCE:genetic interaction:GO:0006452
translational frameshifting [PMID:19424157]"
/experiment="EXISTENCE:genetic interaction:GO:0045901
positive regulation of translational elongation
[PMID:19338753|PMID:19424157]"
/experiment="EXISTENCE:mutant phenotype:GO:0003746
translation elongation factor activity [PMID:19424157]"
/experiment="EXISTENCE:mutant phenotype:GO:0045901
positive regulation of translational elongation
[PMID:19338753|PMID:19424157]"
/experiment="EXISTENCE:mutant phenotype:GO:1903272
positive regulation of cytoplasmic translational
elongation through polyproline stretches [PMID:24923804]"
/note="Translation elongation factor eIF-5A; required for
translation of proteins containing polyproline stretches;
structural homolog of bacterial EF-P; undergoes an
essential hypusination modification; HYP2 has paralog ANB1
that arose from whole genome duplication; in humans EIF5A
variants cause a disorder characterized by variable
combinations of developmental delay, microcephaly,
micrognathia and dysmorphism; human EIF5A complements
inviability of yeast hyp2 anb1 double null mutant"
/codon_start=1
/product="translation elongation factor eIF-5A"
/protein_id="NP_010880.3"
/db_xref="GeneID:856677"
/db_xref="SGD:S000000760"
/translation="MSDEEHTFETADAGSSATYPMQCSALRKNGFVVIKSRPCKIVDM
STSKTGKHGHAKVHLVAIDIFTGKKLEDLSPSTHNMEVPVVKRNEYQLLDIDDGFLSL
MNMDGDTKDDVKAPEGELGDSLQTAFDEGKDLMVTIISAMGEEAAISFKEAARTD"
gene <86179..>86598
/gene="MTC7"
/locus_tag="YEL033W"
/db_xref="GeneID:856678"
mRNA <86179..>86598
/gene="MTC7"
/locus_tag="YEL033W"
/product="Mtc7p"
/transcript_id="NM_001178848.1"
/db_xref="GeneID:856678"
CDS 86179..86598
/gene="MTC7"
/locus_tag="YEL033W"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:40704575]"
/note="hypothetical protein; predicted metabolic role
based on network analysis derived from ChIP experiments, a
large-scale deletion study and localization of
transcription factor binding sites; null mutant is
sensitive to temperature oscillation in a cdc13-1 mutant"
/codon_start=1
/product="Mtc7p"
/protein_id="NP_010881.1"
/db_xref="GeneID:856678"
/db_xref="SGD:S000000759"
/translation="MKKEKKTPTPLPSHHVLFAEPGFFLCNFFFVLLKHTQINPFFYF
LFILLFIIYIAIIYFVFIRISHFSFSLCRQCNSLGRMIFMCAYLPAASSRSVANPALP
PQKKKKKKKKGTLRTGEVEEQAKGNISFDLCGKQNFQ"
gene 86604..86685
/locus_tag="YNCE0005W"
/db_xref="GeneID:856679"
tRNA 86604..86685
/locus_tag="YNCE0005W"
/product="tRNA-Ser"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0030371 translation
repressor activity [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043555 regulation
of translation in response to stress [PMID:27609601]"
/note="Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:856679"
/db_xref="SGD:S000006724"
gene <86937..>89852
/gene="MCM3"
/locus_tag="YEL032W"
/db_xref="GeneID:856680"
mRNA <86937..>89852
/gene="MCM3"
/locus_tag="YEL032W"
/product="MCM DNA helicase complex subunit MCM3"
/transcript_id="NM_001178847.1"
/db_xref="GeneID:856680"
CDS 86937..89852
/gene="MCM3"
/locus_tag="YEL032W"
/EC_number="3.6.4.12"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:9554851]"
/experiment="EXISTENCE:direct assay:GO:0003688 DNA
replication origin binding [PMID:16824194|PMID:11756674]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10704410|PMID:8224843]"
/experiment="EXISTENCE:direct assay:GO:0005656 nuclear
pre-replicative complex [PMID:16824194|PMID:9335335]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276|PMID:8224843|PMID:10704410]"
/experiment="EXISTENCE:direct assay:GO:0006267
pre-replicative complex assembly involved in nuclear cell
cycle DNA replication [PMID:16824194]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:12480933]"
/experiment="EXISTENCE:direct assay:GO:0017116
single-stranded DNA helicase activity [PMID:18657510]"
/experiment="EXISTENCE:direct assay:GO:0042555 MCM complex
[PMID:12480933]"
/experiment="EXISTENCE:direct assay:GO:0071162 CMG complex
[PMID:26524492]"
/experiment="EXISTENCE:direct assay:GO:1904931 MCM complex
binding [PMID:26912723]"
/experiment="EXISTENCE:mutant phenotype:GO:0000727
double-strand break repair via break-induced replication
[PMID:20516198]"
/experiment="EXISTENCE:mutant phenotype:GO:0006270 DNA
replication initiation [PMID:12060653]"
/experiment="EXISTENCE:mutant phenotype:GO:0006271 DNA
strand elongation involved in DNA replication
[PMID:10834843]"
/experiment="EXISTENCE:mutant phenotype:GO:0016887 ATP
hydrolysis activity [PMID:12480933]"
/experiment="EXISTENCE:mutant phenotype:GO:0030466 silent
mating-type cassette heterochromatin formation
[PMID:19064704]"
/experiment="EXISTENCE:mutant phenotype:GO:0031509
subtelomeric heterochromatin formation [PMID:19064704]"
/experiment="EXISTENCE:mutant phenotype:GO:1902975 mitotic
DNA replication initiation [PMID:8224843]"
/experiment="EXISTENCE:physical interaction:GO:0031261 DNA
replication preinitiation complex [PMID:9554851]"
/note="Protein involved in DNA replication; component of
the Mcm2-7 hexameric helicase complex that binds chromatin
as a part of the pre-replicative complex"
/codon_start=1
/product="MCM DNA helicase complex subunit MCM3"
/protein_id="NP_010882.1"
/db_xref="GeneID:856680"
/db_xref="SGD:S000000758"
/translation="MEGSTGFDGDATTFFAPDAVFGDRVRRFQEFLDTFTSYRDSVRS
IQVYNSNNAANYNDDQDDADERDLLGDDDGDDLEKEKKAASSTSLNILPHRIIISLDD
LREFDRSFWSGILVEPAYFIPPAEKALTDLADSMDDVPHPNASAVSSRHPWKLSFKGS
FGAHALSPRTLTAQHLNKLVSVEGIVTKTSLVRPKLIRSVHYAAKTGRFHYRDYTDAT
TTLTTRIPTPAIYPTEDTEGNKLTTEYGYSTFIDHQRITVQEMPEMAPAGQLPRSIDV
ILDDDLVDKTKPGDRVNVVGVFKSLGAGGMNQSNSNTLIGFKTLILGNTVYPLHARST
GVAARQMLTDFDIRNINKLSKKKDIFDILSQSLAPSIYGHDHIKKAILLMLMGGVEKN
LENGSHLRGDINILMVGDPSTAKSQLLRFVLNTASLAIATTGRGSSGVGLTAAVTTDR
ETGERRLEAGAMVLADRGVVCIDEFDKMTDVDRVAIHEVMEQQTVTIAKAGIHTTLNA
RCSVIAAANPVFGQYDVNRDPHQNIALPDSLLSRFDLLFVVTDDINEIRDRSISEHVL
RTHRYLPPGYLEGEPVRERLNLSLAVGEDADINPEEHSNSGAGVENEGEDDEDHVFEK
FNPLLQAGAKLAKNKGNYNGTEIPKLVTIPFLRKYVQYAKERVIPQLTQEAINVIVKN
YTDLRNDDNTKKSPITARTLETLIRLATAHAKVRLSKTVNKVDAKVAANLLRFALLGE
DIGNDIDEEESEYEEALSKRSPQKSPKKRQRVRQPASNSGSPIKSTPRRSTASSVNAT
PSSARRILRFQDDEQNAGEDDNDIMSPLPADEEAELQRRLQLGLRVSPRRREHLHAPE
EGSSGPLTEVGTPRLPNVSSAGQDDEQQQSVISFDNVEPGTISTGRLSLISGIIARLM
QTEIFEEESYPVASLFERINEELPEEEKFSAQEYLAGLKIMSDRNNLMVADDKVWRV"
gene <90258..>93905
/gene="SPF1"
/locus_tag="YEL031W"
/gene_synonym="COD1; PER9; PIO1"
/db_xref="GeneID:856681"
mRNA <90258..>93905
/gene="SPF1"
/locus_tag="YEL031W"
/gene_synonym="COD1; PER9; PIO1"
/product="ion-transporting P-type ATPase SPF1"
/transcript_id="NM_001178846.3"
/db_xref="GeneID:856681"
CDS 90258..93905
/gene="SPF1"
/locus_tag="YEL031W"
/gene_synonym="COD1; PER9; PIO1"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane
[PMID:30785834|PMID:12058017|PMID:22745129]"
/experiment="EXISTENCE:direct assay:GO:0005801 cis-Golgi
network [PMID:11791712]"
/experiment="EXISTENCE:direct assay:GO:0070273
phosphatidylinositol-4-phosphate binding [PMID:30785834]"
/experiment="EXISTENCE:mutant phenotype:GO:0006874
intracellular calcium ion homeostasis [PMID:12058017]"
/experiment="EXISTENCE:mutant phenotype:GO:0030026
intracellular manganese ion homeostasis [PMID:24392018]"
/experiment="EXISTENCE:mutant phenotype:GO:0055092 sterol
homeostasis [PMID:22918956|PMID:30785834]"
/note="P-type ATPase, ion transporter of the ER membrane;
required to maintain normal lipid and sterol composition
of intracellular compartments and proper targeting of
mitochondrial outer membrane tail-anchored proteins;
involved in ER function and Ca2+ homeostasis; required for
regulating Hmg2p degradation; confers sensitivity to a
killer toxin (SMKT) produced by Pichia farinosa KK1; null
mutation is complemented by human Parkinson
disease-related ATP13A1"
/codon_start=1
/product="ion-transporting P-type ATPase SPF1"
/protein_id="NP_010883.3"
/db_xref="GeneID:856681"
/db_xref="SGD:S000000757"
/translation="MTKKSFVSSPIVRDSTLLVPKSLIAKPYVLPFFPLYATFAQLYF
QQYDRYIKGPEWTFVYLGTLVSLNILVMLMPAWNVKIKAKFNYSTTKNVNEATHILIY
TTPNNGSDGIVEIQRVTEAGSLQTFFQFQKKRFLWHENEQVFSSPKFLVDESPKIGDF
QKCKGHSGDLTHLKRLYGENSFDIPIPTFMELFKEHAVAPLFVFQVFCVALWLLDEFW
YYSLFNLFMIISMEAAAVFQRLTALKEFRTMGIKPYTINVFRNKKWVALQTNELLPMD
LVSITRTAEESAIPCDLILLDGSAIVNEAMLSGESTPLLKESIKLRPSEDNLQLDGVD
KIAVLHGGTKALQVTPPEHKSDIPPPPDGGALAIVTKTGFETSQGSLVRVMIYSAERV
SVDNKEALMFILFLLIFAVIASWYVWVEGTKMGRIQSKLILDCILIITSVVPPELPME
LTMAVNSSLAALAKFYVYCTEPFRIPFAGRIDVCCFDKTGTLTGEDLVFEGLAGISAD
SENIRHLYSAAEAPESTILVIGAAHALVKLEDGDIVGDPMEKATLKAVGWAVERKNSN
YREGTGKLDIIRRFQFSSALKRSASIASHNDALFAAVKGAPETIRERLSDIPKNYDEI
YKSFTRSGSRVLALASKSLPKMSQSKIDDLNRDDVESELTFNGFLIFHCPLKDDAIET
IKMLNESSHRSIMITGDNPLTAVHVAKEVGIVFGETLILDRAGKSDDNQLLFRDVEET
VSIPFDPSKDTFDHSKLFDRYDIAVTGYALNALEGHSQLRDLLRHTWVYARVSPSQKE
FLLNTLKDMGYQTLMCGDGTNDVGALKQAHVGIALLNGTEEGLKKLGEQRRLEGMKMM
YIKQTEFMARWNQPQPPVPEPIAHLFPPGPKNPHYLKALESKGTVITPEIRKAVEEAN
SKPVEVIKPNGLSEKKPADLASLLLNSAGDAQGDEAPALKLGDASCAAPFTSKLANVS
AVTNIIRQGRCALVNTIQMYKILALNCLISAYSLSIIYMAGVKFGDGQATVSGLLLSV
CFLSISRGKPLEKLSKQRPQSGIFNVYIMGSILSQFAVHIATLVYITTEIYKLEPREP
QVDLEKEFAPSLLNTGIFIIQLVQQVSTFAVNYQGEPFRENIRSNKGMYYGLLGVTGL
ALASATEFLPELNEAMKFVPMTDDFKIKLTLTLLLDFFGSWGVEHFFKFFFMDDKPSD
ISVQQVKIASK"
rep_origin 93978..94218
/note="ARS508; Autonomously Replicating Sequence"
/db_xref="SGD:S000077381"
gene <94644..>96578
/gene="ECM10"
/locus_tag="YEL030W"
/gene_synonym="SSC3"
/db_xref="GeneID:856682"
mRNA <94644..>96578
/gene="ECM10"
/locus_tag="YEL030W"
/gene_synonym="SSC3"
/product="Hsp70 family ATPase ECM10"
/transcript_id="NM_001178845.1"
/db_xref="GeneID:856682"
CDS 94644..96578
/gene="ECM10"
/locus_tag="YEL030W"
/gene_synonym="SSC3"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:14576278|PMID:11150530|PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0006626 protein
targeting to mitochondrion [PMID:11150530]"
/experiment="EXISTENCE:direct assay:GO:0042645
mitochondrial nucleoid [PMID:12943685]"
/note="Heat shock protein of the Hsp70 family; localized
in mitochondrial nucleoids, plays a role in protein
translocation, interacts with Mge1p in an ATP-dependent
manner; overexpression induces extensive mitochondrial DNA
aggregations; ECM10 has a paralog, SSC1, that arose from
the whole genome duplication"
/codon_start=1
/product="Hsp70 family ATPase ECM10"
/protein_id="NP_010884.1"
/db_xref="GeneID:856682"
/db_xref="SGD:S000000756"
/translation="MLPSWKAFKAHNILRILTRFQSTKIPDAVIGIDLGTTNSAVAIM
EGKVPRIIENAEGSRTTPSVVAFTKDGERLVGEPAKRQSVINSENTLFATKRLIGRRF
EDAEVQRDINQVPFKIVKHSNGDAWVEARNRTYSPAQIGGFILNKMKETAEAYLAKSV
KNAVVTVPAYFNDAQRQATKDAGQIIGLNVLRVVNEPTAAALAYGLDKSEPKVIAVFD
LGGGTFDISILDIDNGIFEVKSTNGDTHLGGEDFDIYLLQEIISHFKKETGIDLSNDR
MAVQRIREAAEKAKIELSSTLSTEINLPFITADAAGPKHIRMPFSRVQLENITAPLID
RTVDPVKKALKDARITASDISDVLLVGGMSRMPKVADTVKKLFGKDASKAVNPDEAVA
LGAAIQAAVLSGEVTDVLLLDVTPLSLGIETLGGVFTKLIPRNSTIPNKKSQIFSTAA
SGQTSVEVKVFQGERELVKDNKLIGNFTLAGIPPAPKGTPQIEVTFDIDANGIINVSA
KDLASHKDSSITVAGASGLSDTEIDRMVNEAERYKNQDRARRNAIETANKADQLANDT
ENSIKEFEGKLDKTDSQRLKDQISSLRELVSRSQAGDEVNDDDVGTKIDNLRTSSMKL
FEQLYKNSDNPETKNGRENK"
gene complement(<96858..>97796)
/gene="BUD16"
/locus_tag="YEL029C"
/db_xref="GeneID:856683"
mRNA complement(<96858..>97796)
/gene="BUD16"
/locus_tag="YEL029C"
/product="putative pyridoxal kinase BUD16"
/transcript_id="NM_001178844.1"
/db_xref="GeneID:856683"
CDS complement(96858..97796)
/gene="BUD16"
/locus_tag="YEL029C"
/EC_number="2.7.1.35"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:36164978]"
/experiment="EXISTENCE:genetic interaction:GO:0042823
pyridoxal phosphate biosynthetic process [PMID:17696614]"
/experiment="EXISTENCE:mutant phenotype:GO:0042823
pyridoxal phosphate biosynthetic process [PMID:17696614]"
/note="Putative pyridoxal kinase; a key enzyme involved in
pyridoxal 5'-phosphate synthesis, the active form of
vitamin B6; required for genome integrity; involved in
bud-site selection; localizes to cytosol and peroxisomes;
similarity to yeast BUD17 and human pyridoxal kinase
(PDXK)"
/codon_start=1
/product="putative pyridoxal kinase BUD16"
/protein_id="NP_010885.1"
/db_xref="GeneID:856683"
/db_xref="SGD:S000000755"
/translation="MPRLLATQSHVVHGYVGNKAATFPLQCLGWDVDCCNSVQFSNHT
GYGLDKVFGTITRETDLKELLSGLFDNFSQDYQALLSGYLPNKNSVRCMGTYYAKFKE
ANPEMIWLMDPVMGDEGQLYVSEDVIPEYRKLALSPKQLVDIITPNQFELEILYGGEI
KTKEHLKKALKKLHQTIPVIIVTSCDCKMFDDKDFIYCVASMEGKTPIVYRVPFIDSY
FTGVGDLFSALLLDRVYKILSNPTTTLKFEDQVNNVLNVIQKVLKITRSYASGKMKAK
MGSALEMKEMELRLIESRDIYETINIHQTDYIYARL"
gene <98668..>99129
/locus_tag="YEL028W"
/db_xref="GeneID:856684"
mRNA <98668..>99129
/locus_tag="YEL028W"
/product="uncharacterized protein"
/transcript_id="NM_001348820.1"
/db_xref="GeneID:856684"
CDS 98668..99129
/locus_tag="YEL028W"
/note="hypothetical protein; conserved among S. cerevisiae
strains; YEL028W is not an essential gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335764.1"
/db_xref="GeneID:856684"
/db_xref="SGD:S000000754"
/translation="MKITITSLLFFLVMIVELASAGTLLHNGANLPSLRDNTTLTDAR
NVLKYLQVLGFPSNKIAATDTVGTFIIFSNRTEANTTAMTKTVSYCYRNYGHSFYFTH
YKYDYFPSGISYMAKLGDATVNHTDLPHFRNNKRLTTQELNAFQHPIVEFQ"
repeat_region complement(99490..99664)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006886"
gene 100133..100204
/gene="IMT4"
/locus_tag="YNCE0006W"
/db_xref="GeneID:856685"
tRNA 100133..100204
/gene="IMT4"
/locus_tag="YNCE0006W"
/product="tRNA-Met"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006413
translational initiation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0016282 eukaryotic
43S preinitiation complex [PMID:12008673]"
/experiment="EXISTENCE:direct assay:GO:0033290 eukaryotic
48S preinitiation complex [PMID:17242201]"
/experiment="EXISTENCE:direct assay:GO:0071074 eukaryotic
initiation factor eIF2 binding [PMID:14698289]"
/note="Methionine initiator tRNA (tRNA-Met); predicted by
tRNAscan-SE analysis; one of four initiator methionine
tRNAs in yeast that are functional for translation"
/db_xref="GeneID:856685"
/db_xref="SGD:S000006660"
gene <100769..>101251
/gene="VMA3"
/locus_tag="YEL027W"
/gene_synonym="CLS7; CUP5; GEF2"
/db_xref="GeneID:856686"
mRNA <100769..>101251
/gene="VMA3"
/locus_tag="YEL027W"
/gene_synonym="CLS7; CUP5; GEF2"
/product="H(+)-transporting V0 sector ATPase subunit c"
/transcript_id="NM_001178842.3"
/db_xref="GeneID:856686"
CDS 100769..101251
/gene="VMA3"
/locus_tag="YEL027W"
/gene_synonym="CLS7; CUP5; GEF2"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:15252052]"
/experiment="EXISTENCE:mutant phenotype:GO:0006623 protein
targeting to vacuole [PMID:2145283]"
/experiment="EXISTENCE:mutant phenotype:GO:0006878
intracellular copper ion homeostasis [PMID:9434348]"
/experiment="EXISTENCE:mutant phenotype:GO:0006879
intracellular iron ion homeostasis [PMID:9434348]"
/experiment="EXISTENCE:mutant phenotype:GO:0006897
endocytosis [PMID:2145283]"
/experiment="EXISTENCE:mutant phenotype:GO:0007033 vacuole
organization [PMID:2145283]"
/experiment="EXISTENCE:mutant phenotype:GO:0007035
vacuolar acidification [PMID:2145283]"
/note="Proteolipid subunit c of the V0 domain of vacuolar
H(+)-ATPase; dicyclohexylcarbodiimide binding subunit;
required for vacuolar acidification and important for
copper and iron metal ion homeostasis"
/codon_start=1
/product="H(+)-transporting V0 sector ATPase subunit c"
/protein_id="NP_010887.3"
/db_xref="GeneID:856686"
/db_xref="SGD:S000000753"
/translation="MTELCPVYAPFFGAIGCASAIIFTSLGAAYGTAKSGVGICATCV
LRPDLLFKNIVPVIMAGIIAIYGLVVSVLVCYSLGQKQALYTGFIQLGAGLSVGLSGL
AAGFAIGIVGDAGVRGSSQQPRLFVGMILILIFAEVLGLYGLIVALLLNSRATQDVVC
"
gene <101943..>102323
/gene="SNU13"
/locus_tag="YEL026W"
/db_xref="GeneID:856687"
mRNA <101943..>102323
/gene="SNU13"
/locus_tag="YEL026W"
/product="RNA binding protein SNU13"
/transcript_id="NM_001178841.1"
/db_xref="GeneID:856687"
CDS 101943..102323
/gene="SNU13"
/locus_tag="YEL026W"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:10684247]"
/experiment="EXISTENCE:direct assay:GO:0030621 U4 snRNA
binding [PMID:17631273]"
/experiment="EXISTENCE:direct assay:GO:0031428 box C/D
methylation guide snoRNP complex [PMID:11081632]"
/experiment="EXISTENCE:direct assay:GO:0032040
small-subunit processome [PMID:12068309]"
/experiment="EXISTENCE:direct assay:GO:0034511 U3 snoRNA
binding [PMID:17631273]"
/experiment="EXISTENCE:direct assay:GO:0046540 U4/U6 x U5
tri-snRNP complex [PMID:10449419|PMID:10377396]"
/experiment="EXISTENCE:mutant phenotype:GO:0000398 mRNA
splicing, via spliceosome [PMID:14730029|PMID:11720284]"
/experiment="EXISTENCE:mutant phenotype:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:14730029]"
/experiment="EXISTENCE:mutant phenotype:GO:0003723 RNA
binding [PMID:14730029]"
/note="RNA binding protein; part of U3 snoRNP involved in
rRNA processing, part of U4/U6-U5 tri-snRNP involved in
mRNA splicing, similar to human 15.5K protein"
/codon_start=1
/product="RNA binding protein SNU13"
/protein_id="NP_010888.1"
/db_xref="GeneID:856687"
/db_xref="SGD:S000000752"
/translation="MSAPNPKAFPLADAALTQQILDVVQQAANLRQLKKGANEATKTL
NRGISEFIIMAADCEPIEILLHLPLLCEDKNVPYVFVPSRVALGRACGVSRPVIAASI
TTNDASAIKTQIYAVKDKIETLLI"
gene complement(<102581..>106147)
/locus_tag="YEL025C"
/db_xref="GeneID:856688"
mRNA complement(<102581..>106147)
/locus_tag="YEL025C"
/product="uncharacterized protein"
/transcript_id="NM_001178840.1"
/db_xref="GeneID:856688"
CDS complement(102581..106147)
/locus_tag="YEL025C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:26928762|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to both the cytoplasm and
the nucleus"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_010889.1"
/db_xref="GeneID:856688"
/db_xref="SGD:S000000751"
/translation="MARQDETDCKKLDSNSAFLPLCITSYRSKDEAEKLVSCLHHEGS
YFLCFSYGLYTGTSNELKAKVEFALDGPFVVNYATVPMFMSASFCHLLMFNDGSIKGF
KCENNKFEIIYDSNVAPKLDTISLLTSSWLNASFEGVLYTCNKNISYDSDLHTLCTTI
YSFDFREGIVERFYATDGEEIISFDFISREDLLDDATCTGKSHVYFLCLLKSCYSENL
FLKEYNLAGKHGSRKFDLNEYRQYNLLPICDNEFCYMKVVCGHTIVVLTTNYTQIINI
KASGLTNGAFFNNKGLPNVENYQFLKDSYDVIYERSVILLTISDVYANKYTAKIHTRS
LHRDHVLKELQWTKERVFKPPKHDLCDVILQLPREKYIAVTRINGVNFISKDHRGSKL
EKVKSGPAYTNKVYLASQVIGNKGTDIDSLLLGGSFNSRRGFLEKKILVYDKTLFKLV
TSSKALVENVTDFWVTDLALNGGDEFAYESGGLIYKNGIFLMDEPYDYGLFVSRTGKV
LKAGMDGSTGEFRQVDFLQSNHNSSTMFCYPVQNSKLRISILEAKSGFLRKKEEFFIH
GLNSKESIVSCFSDGAEKYLFVIYLDGVFSVWNANQKKVATSHPDYSFIAYDQLIKIS
WLSKKHKHDSIYVIASSYTGCVRVYKSESNFLRVDLEIHSLYDQKLELLDTIPSLPLV
FLYNDKEIILLNLQNMSYGCIQLGLVPRRMRIRPGKALFSLCVLDYDSRISIFEFGST
FYREGFTKQMTSLKPQLENHIFYLPSIPVELYTVPNNLNQAVVCLVDSNSRQYKLMLF
NYASMKAVSTFSFSDEKYLHAVVKPLWPEQNSIYLSQRPFYGNKFVVCLGVGEKRTKF
WLFEIRNNNITQLYANYLEDCIFSVFIYYECNLVLFSGDSGIAAYKINMLKEGAEILE
AYSFPALSSINHIGLPAYMSGDYLVQFEILRDFLRTRFPIRTSIVYPSEYPECPYLGF
KRLGSITQVATKIIPKKLREPKNDCLASDKNYLLGRLSDLSCATLKYSNRSYVATIGI
DNTLTIYEDSKLSVDKGGLAMPYLKIRLPNKIISLAAIPDGFQNLQICPNFNSQRLEG
VIPLFLLCGTEGQIYIISEFIGELWMRTLHDYKKIKLECKRAFRRSEKSMRNVTKQYS
VSKETGINESGFDELARNSKRRHIDHSPYKTIDFFDPVKLKR"
gene <107260..>107907
/gene="RIP1"
/locus_tag="YEL024W"
/db_xref="GeneID:856689"
mRNA <107260..>107907
/gene="RIP1"
/locus_tag="YEL024W"
/product="ubiquinol--cytochrome-c reductase catalytic
subunit RIP1"
/transcript_id="NM_001178839.3"
/db_xref="GeneID:856689"
CDS 107260..107907
/gene="RIP1"
/locus_tag="YEL024W"
/EC_number="7.1.1.8"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0006122
mitochondrial electron transport, ubiquinol to cytochrome
c [PMID:3036836]"
/experiment="EXISTENCE:direct assay:GO:0045275 respiratory
chain complex III [PMID:10873857]"
/experiment="EXISTENCE:mutant phenotype:GO:0008121
quinol-cytochrome-c reductase activity [PMID:2537289]"
/experiment="EXISTENCE:mutant phenotype:GO:0009060 aerobic
respiration [PMID:3036836]"
/experiment="EXISTENCE:mutant phenotype:GO:0045275
respiratory chain complex III [PMID:3036836]"
/experiment="EXISTENCE:physical interaction:GO:0005739
mitochondrion [PMID:16962558]"
/note="Ubiquinol-cytochrome-c reductase; a Rieske
iron-sulfur protein of the mitochondrial cytochrome bc1
complex; transfers electrons from ubiquinol to cytochrome
c1 during respiration; during import, Rip1p is first
imported into the mitochondrial matrix where it is
processed, acquires its Fe-S cluster, and is folded, then
is translocated into the inner membrane by the action of a
homo-oligomer of Bcs1p, and finally is delivered by Bcs1p
to Complex III for assembly"
/codon_start=1
/product="ubiquinol--cytochrome-c reductase catalytic
subunit RIP1"
/protein_id="NP_010890.3"
/db_xref="GeneID:856689"
/db_xref="SGD:S000000750"
/translation="MLGIRSSVKTCFKPMSLTSKRLISQSLLASKSTYRTPNFDDVLK
ENNDADKGRSYAYFMVGAMGLLSSAGAKSTVETFISSMTATADVLAMAKVEVNLAAIP
LGKNVVVKWQGKPVFIRHRTPHEIQEANSVDMSALKDPQTDADRVKDPQWLIMLGICT
HLGCVPIGEAGDFGGWFCPCHGSHYDISGRIRKGPAPLNLEIPAYEFDGDKVIVG"
gene complement(<108504..>110552)
/locus_tag="YEL023C"
/db_xref="GeneID:856690"
mRNA complement(<108504..>110552)
/locus_tag="YEL023C"
/product="uncharacterized protein"
/transcript_id="NM_001178838.1"
/db_xref="GeneID:856690"
CDS complement(108504..110552)
/locus_tag="YEL023C"
/note="hypothetical protein; expression is increased
greatly during sporulation; YEL023C is not an essential
gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_010891.1"
/db_xref="GeneID:856690"
/db_xref="SGD:S000000749"
/translation="MDSFNYIHGKYKKNGTGGDRSINPSSHSSSGKNIILCFDGTREN
FGPQPFTNILKLYNLLENGDSSEQICYYQPGIGSVGFDAVVDVRRRLTISHLQNLLDS
MFAFSLDNHICSAYLFLMKYFEPGDRIYMFGFSRGAFIARVLAGMIERVGLLSKGLEE
MVKMAWQIYEKWEYDSQPNELQYTSTLAEEFKKTFSRDYEVKIHFQGLFDSVNSVGIL
RDRLFPCTQRSNIVEHVRHCVSLDERRGKFKQLCFTPMPYIPKLFSLTYCNHITDQCS
PVPTSNALMRDLTPENPLIKYTLKSGAHSISNPSPLIPDNPGRLLSSKSEETTELLLD
LNSFLEGNSYARDTECSTRGIEAIFQLQSIQGSGTSSRMTMTPDLIEKWFPGDHSDVG
GGWAPDCETEENLSNLTLRWILAEAIKFGVKFKPGAIHDFATKHTSIGSLFADTHDYL
SFNSPKKCSLLGVSDNEDGAREDKSGRNERMEDCLKNIKETRLSLKDEKEKVKDAFTL
KCGHANKFMRLVWWVLELLPIGIRMENKEGKWQNFHTPNLGRSRYVPEYVSLHWSVYW
RIKFDRRYRPDNMPEYVRQLFQDLEGIDLKSNKVSNKYDKQDNSNGSEINGGFFDNEE
GQELHMGQKASYFATTYNSRLFDSKYSQLKKKFMDWDSNSWTDIPDDLKIYLQQDESL
"
gene <111421..>115800
/gene="GEA2"
/locus_tag="YEL022W"
/db_xref="GeneID:856691"
mRNA <111421..>115800
/gene="GEA2"
/locus_tag="YEL022W"
/product="Arf family guanine nucleotide exchange factor
GEA2"
/transcript_id="NM_001178837.1"
/db_xref="GeneID:856691"
CDS 111421..115800
/gene="GEA2"
/locus_tag="YEL022W"
/experiment="EXISTENCE:direct assay:GO:0000137 Golgi cis
cisterna [PMID:11294905]"
/experiment="EXISTENCE:direct assay:GO:0005085
guanyl-nucleotide exchange factor activity
[PMID:10430582]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0006890
retrograde vesicle-mediated transport, Golgi to
endoplasmic reticulum [PMID:11294905]"
/experiment="EXISTENCE:genetic interaction:GO:0030036
actin cytoskeleton organization [PMID:14668359]"
/experiment="EXISTENCE:mutant phenotype:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:11493664]"
/experiment="EXISTENCE:mutant phenotype:GO:0006891
intra-Golgi vesicle-mediated transport [PMID:11493664]"
/experiment="EXISTENCE:mutant phenotype:GO:0016236
macroautophagy [PMID:20444982]"
/experiment="EXISTENCE:mutant phenotype:GO:0033363
secretory granule organization [PMID:14734650]"
/note="Guanine nucleotide exchange factor for ADP
ribosylation factors (ARFs); involved in vesicular
transport between the Golgi and ER, Golgi organization,
and actin cytoskeleton organization; GEA2 has a paralog,
GEA1, that arose from the whole genome duplication"
/codon_start=1
/product="Arf family guanine nucleotide exchange factor
GEA2"
/protein_id="NP_010892.1"
/db_xref="GeneID:856691"
/db_xref="SGD:S000000748"
/translation="MSDREFVTVDPVTIIIKECINLSTAMRKYSKFTSQSGVAALLGG
GSEIFSNQDDYLAHTFNNLNTNKHNDPFLSGFIQLRLMLNKLKNLDNIDSLTILQPFL
LIVSTSSISGYITSLALDSLQKFFTLNIINESSQNYIGAHRATVNALTHCRFEGSQQL
SDDSVLLKVVFLLRSIVDSPYGDLLSNSIIYDVLQTILSLACNNRRSEVLRNAAQSTM
IAVTVKIFSKLKTIEPVNVNQIYINDESYTNDVLKADTIGTNVESKEEGSQEDPIGMK
VNNEEAISEDDGIEEEHIHSEKSTNGAEQLDIVQKTTRSNSRIQAYADDNYGLPVVRQ
YLNLLLSLIAPENELKHSYSTRIFGLELIQTALEISGDRLQLYPRLFTLISDPIFKSI
LFIIQNTTKLSLLQATLQLFTTLVVILGNNLQLQIELTLTRIFSILLDDGTANNSSSE
NKNKPSIIKELLIEQISILWTRSPSFFTSTFINFDCNLDRADVSINFLKALTKLALPE
SALTTTESVPPICLEGLVSLVDDMFDHMKDIDREEFGRQKNEMEILKKRDRKTEFIEC
TNAFNEKPKKGIPMLIEKGFIASDSDKDIAEFLFNNNNRMNKKTIGLLLCHPDKVSLL
NEYIRLFDFSGLRVDEAIRILLTKFRLPGESQQIERIIEAFSSAYCENQDYDPSKISD
NAEDDISTVQPDADSVFILSYSIIMLNTDLHNPQVKEHMSFEDYSGNLKGCCNHKDFP
FWYLDRIYCSIRDKEIVMPEEHHGNEKWFEDAWNNLISSTTVITEIKKDTQSVMDKLT
PLELLNFDRAIFKQVGPSIVSTLFNIYVVASDDHISTRMITSLDKCSYISAFFDFKDL
FNDILNSIAKGTTLINSSHDDELSTLAFEYGPMPLVQIKFEDTNTEIPVSTDAVRFGR
SFKGQLNTVVFFRIIRRNKDPKIFSKELWLNIVNIILTLYEDLILSPDIFPDLQKRLK
LSNLPKPSPEISINKSKESKGLLSTFASYLKGDEEPTEEEIKSSKKAMECIKSSNIAA
SVFGNESNITADLIKTLLDSAKTEKNADNSRYFEAELLFIIELTIALFLFCKEEKELG
KFILQKVFQLSHTKGLTKRTVRRMLTYKILLISLCADQTEYLSKLINDELLKKGDIFT
QKFFATNQGKEFLKRLFSLTESEFYRGFLLGNENFWKFLRKVTAMKEQSESIFEYLNE
SIKTDSNILTNENFMWVLGLLDEISSMGAVGNHWEIEYKKLTESGHKIDKENPYKKSI
ELSLKSIQLTSHLLEDNNDLRKNEIFAIIQALAHQCINPCKQISEFAVVTLEQTLINK
IEIPTNEMESVEELIEGGLLPLLNSSETQEDQKILISSILTIISNVYLHYLKLGKTSN
ETFLKILSIFNKFVEDSDIEKKLQQLILDKKSIEKGNGSSSHGSAHEQTPESNDVEIE
ATAPIDDNTDDDNKPKLSDVEKD"
gene <116167..>116970
/gene="URA3"
/locus_tag="YEL021W"
/db_xref="GeneID:856692"
mRNA <116167..>116970
/gene="URA3"
/locus_tag="YEL021W"
/product="orotidine-5'-phosphate decarboxylase"
/transcript_id="NM_001178836.3"
/db_xref="GeneID:856692"
CDS 116167..116970
/gene="URA3"
/locus_tag="YEL021W"
/EC_number="4.1.1.23"
/experiment="EXISTENCE:direct assay:GO:0004590
orotidine-5'-phosphate decarboxylase activity
[PMID:2061334]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:18812321]"
/experiment="EXISTENCE:direct assay:GO:0006222 UMP
biosynthetic process [PMID:2061334]"
/experiment="EXISTENCE:mutant phenotype:GO:0004590
orotidine-5'-phosphate decarboxylase activity
[PMID:5651325|PMID:4550660]"
/experiment="EXISTENCE:mutant phenotype:GO:0006207 'de
novo' pyrimidine nucleobase biosynthetic process
[PMID:4550660|PMID:5651325]"
/experiment="EXISTENCE:mutant phenotype:GO:0006222 UMP
biosynthetic process [PMID:5651325|PMID:4550660]"
/note="Orotidine-5'-phosphate (OMP) decarboxylase;
catalyzes the sixth enzymatic step in the de novo
biosynthesis of pyrimidines, converting OMP into uridine
monophosphate (UMP); converts 5-FOA into 5-fluorouracil, a
toxic compound"
/codon_start=1
/product="orotidine-5'-phosphate decarboxylase"
/protein_id="NP_010893.3"
/db_xref="GeneID:856692"
/db_xref="SGD:S000000747"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLA
TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
gene <117211..>117474
/gene="TIM9"
/locus_tag="YEL020W-A"
/db_xref="GeneID:856693"
mRNA <117211..>117474
/gene="TIM9"
/locus_tag="YEL020W-A"
/product="protein transporter TIM9"
/transcript_id="NM_001184349.1"
/db_xref="GeneID:856693"
CDS 117211..117474
/gene="TIM9"
/locus_tag="YEL020W-A"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0042719
mitochondrial intermembrane space chaperone complex
[PMID:9889188|PMID:9822593]"
/experiment="EXISTENCE:direct assay:GO:0042721 TIM22
mitochondrial import inner membrane insertion complex
[PMID:9495346]"
/experiment="EXISTENCE:direct assay:GO:0045039 protein
insertion into mitochondrial inner membrane
[PMID:10469659|PMID:9822593]"
/experiment="EXISTENCE:direct assay:GO:0051082 unfolded
protein binding [PMID:12138093]"
/experiment="EXISTENCE:direct assay:GO:0140318 protein
transporter activity [PMID:9822593]"
/experiment="EXISTENCE:genetic interaction:GO:0045039
protein insertion into mitochondrial inner membrane
[PMID:9822593]"
/experiment="EXISTENCE:genetic interaction:GO:0140318
protein transporter activity [PMID:9822593]"
/experiment="EXISTENCE:mutant phenotype:GO:0045039 protein
insertion into mitochondrial inner membrane
[PMID:9889188]"
/note="Essential protein of the mitochondrial
intermembrane space; forms a complex with Tim10p (TIM10
complex) that delivers hydrophobic proteins to the TIM22
complex for insertion into the inner membrane"
/codon_start=1
/product="protein transporter TIM9"
/protein_id="NP_010894.1"
/db_xref="GeneID:856693"
/db_xref="SGD:S000007256"
/translation="MDALNSKEQQEFQKVVEQKQMKDFMRLYSNLVERCFTDCVNDFT
TSKLTNKEQTCIMKCSEKFLKHSERVGQRFQEQNAALGQGLGR"
gene complement(117667..118035)
/gene="RPR1"
/locus_tag="YNCE0007C"
/db_xref="GeneID:9164884"
ncRNA complement(117667..118035)
/ncRNA_class="RNase_P_RNA"
/gene="RPR1"
/locus_tag="YNCE0007C"
/product="RPR1"
/experiment="EXISTENCE:direct assay:GO:0004526
ribonuclease P activity [PMID:9620854|PMID:19095620]"
/experiment="EXISTENCE:direct assay:GO:0005655 nucleolar
ribonuclease P complex [PMID:19095620|PMID:9620854]"
/experiment="EXISTENCE:direct assay:GO:0008033 tRNA
processing [PMID:9620854]"
/experiment="EXISTENCE:direct assay:GO:0034965 intronic
box C/D snoRNA processing [PMID:18713869]"
/experiment="EXISTENCE:mutant phenotype:GO:0004526
ribonuclease P activity [PMID:1990278]"
/experiment="EXISTENCE:mutant phenotype:GO:0008033 tRNA
processing [PMID:1990278]"
/experiment="EXISTENCE:mutant phenotype:GO:0034965
intronic box C/D snoRNA processing [PMID:18713869]"
/note="RNA component of nuclear RNase P; nuclear RNase P
cleaves tRNA precursors to generate mature 5' ends and
facilitates turnover of nuclear RNAs; may be responsible
for recognition of substrate tRNAs"
/transcript_id="NR_132166.1"
/db_xref="GeneID:9164884"
/db_xref="SGD:S000006490"
repeat_region complement(118230..118551)
/note="Ty4 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006888"
gene complement(<118617..>120299)
/gene="PXP1"
/locus_tag="YEL020C"
/db_xref="GeneID:856694"
mRNA complement(<118617..>120299)
/gene="PXP1"
/locus_tag="YEL020C"
/product="putative indolepyruvate decarboxylase family
protein"
/transcript_id="NM_001178835.1"
/db_xref="GeneID:856694"
CDS complement(118617..120299)
/gene="PXP1"
/locus_tag="YEL020C"
/EC_number="4.1.2.63"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:27392156|PMID:26928762]"
/note="Peroxisomal matrix protein; well-conserved in
fungi; contains tripartite homology domain of thiamine
pyrophosphate (TPP) enzymes; targeted to peroxisomes by
Pex5p; contains low sequence identity with Pdc1p; mRNA
identified as translated by ribosome profiling data"
/codon_start=1
/product="putative indolepyruvate decarboxylase family
protein"
/protein_id="NP_010895.1"
/db_xref="GeneID:856694"
/db_xref="SGD:S000000746"
/translation="MTTTATQHFAQLLQKYGIDTVFGIVGIPIVQLADTMVANGIKFI
PCRNEQAASYAASAYGYISDKPGVLLIVGGPGLIHALAGIYNSMSNRWPLLVIAGSSS
QSDIHKGGFQELDQVSLLSPFLKFTGKLTPDNIDMITQKALNYCIQGTAGVSYIDVPA
DFIEYEKPLEGNDRTGNELPMILTPNICGPDPSKIKKVVQLILQHKNKNILIVIGKGA
VKNSHEIRRLVNTFNLPFLPTPMAKGIVPDSSPLNVSSARSQALKIADIVLVLGARLN
WILHFGTSPKWNSESIFIQFDSNPETLGDNNVSPGADLSIWGDIGLSVTALVEELTRQ
DSCWKYSGVKQEIREKIQLNQTRLLRKEKTRGAQLNYNQVYGTLRPLIDDYRTILVTE
GANTMDIARISFPTDAPRRRLDAGTNATMGIGLGYALACKASHPELDVVLIQGDSAFG
FSAMEIETAVRCQLALVIVVMNNSGIYHGEKDIEGDLPPTALSKNCRYDLVGKGLGAN
DFFVNTISELSRCFQQAVQLSRTKRETSVINVIIEPGEQKQIAFAWQNKPRL"
gene complement(<120498..>121301)
/gene="MMS21"
/locus_tag="YEL019C"
/gene_synonym="NSE2; PSO10"
/db_xref="GeneID:856695"
mRNA complement(<120498..>121301)
/gene="MMS21"
/locus_tag="YEL019C"
/gene_synonym="NSE2; PSO10"
/product="SUMO ligase MMS21"
/transcript_id="NM_001178834.3"
/db_xref="GeneID:856695"
CDS complement(120498..121301)
/gene="MMS21"
/locus_tag="YEL019C"
/gene_synonym="NSE2; PSO10"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:15738391]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0019789 SUMO
transferase activity [PMID:15738391|PMID:31765407]"
/experiment="EXISTENCE:direct assay:GO:0030915 Smc5-Smc6
complex [PMID:15738391]"
/experiment="EXISTENCE:mutant phenotype:GO:0006281 DNA
repair [PMID:7788712|PMID:15738391]"
/experiment="EXISTENCE:mutant phenotype:GO:0019789 SUMO
transferase activity
[PMID:31765407|PMID:24108357|PMID:15738391]"
/experiment="EXISTENCE:mutant phenotype:GO:0030291 protein
serine/threonine kinase inhibitor activity
[PMID:24108357]"
/experiment="EXISTENCE:mutant phenotype:GO:1990683 DNA
double-strand break attachment to nuclear envelope
[PMID:27056668]"
/note="Highly conserved SUMO E3 ligase subunit of
SMC5-SMC6 complex; required for anchoring dsDNA breaks to
the nuclear periphery; SMC5-SMC6 plays a key role in
removal of X-shaped DNA structures that arise between
sister chromatids during DNA replication and repair;
required for efficient sister chromatid cohesion; mutants
are sensitive to MMS, show increased spontaneous mutation
and mitotic recombination; SUMOylates and inhibits Snf1p
function; supports nucleolar function"
/codon_start=1
/product="SUMO ligase MMS21"
/protein_id="NP_010896.3"
/db_xref="GeneID:856695"
/db_xref="SGD:S000000745"
/translation="MALNDNPIPKSVPLHPKSGKYFHNLHARDLSNIYQQCYKQIDET
INQLVDSTSPSTIGIEEQVADITSTYKLLSTYESESNSFDEHIKDLKKNFKQSSDACP
QIDLSTWDKYRTGELTAPKLSELYLNMPTPEPATMVNNTDTLKILKVLPYIWNDPTCV
IPDLQNPADEDDLQIEGGKIELTCPITCKPYEAPLISRKCNHVFDRDGIQNYLQGYTT
RDCPQAACSQVVSMRDFVRDPIMELRCKIAKMKESQEQDKRSSQAIDVL"
gene <121471..>122310
/gene="EAF5"
/locus_tag="YEL018W"
/db_xref="GeneID:856696"
mRNA <121471..>122310
/gene="EAF5"
/locus_tag="YEL018W"
/product="Eaf5p"
/transcript_id="NM_001178833.1"
/db_xref="GeneID:856696"
CDS 121471..122310
/gene="EAF5"
/locus_tag="YEL018W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095|PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0006281 DNA repair
[PMID:16135807]"
/experiment="EXISTENCE:direct assay:GO:0035267 NuA4
histone acetyltransferase complex [PMID:15485911]"
/note="Non-essential subunit of the NuA4 acetyltransferase
complex; Esa1p-associated factor; relocalizes to the
cytosol in response to hypoxia"
/codon_start=1
/product="Eaf5p"
/protein_id="NP_010897.1"
/db_xref="GeneID:856696"
/db_xref="SGD:S000000744"
/translation="MDKEVSELVVLQLIHTLISNKNEELVRNGGGINMIGNNLRISLV
KLTNEIQNNLLINELTNLRRQSNVANGNRKLGINDILTIVKNLFPEYRTTLNDGQLSL
HGLEMHDIEKLLDEKYDRFKKTQVEQIRMMEDEILKNGIKTGASQLQPHANAGKSGSA
GTSATITTTTPHMAHSMDPKREKLLKLYRDTVLNKLESKTGNFQKLFKSPDGSIIKNE
INYEDIKNETPGSVHELQLILQKSITDGVMRKVIGTDDWKLARQVQFELDDTVQFMRR
ALE"
gene complement(<122798..>122929)
/gene="PMP2"
/locus_tag="YEL017C-A"
/db_xref="GeneID:856697"
mRNA complement(<122798..>122929)
/gene="PMP2"
/locus_tag="YEL017C-A"
/product="proteolipid ATPase"
/transcript_id="NM_001180029.1"
/db_xref="GeneID:856697"
CDS complement(122798..122929)
/gene="PMP2"
/locus_tag="YEL017C-A"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:8063750]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0005886
plasma membrane [PMID:8063750]"
/experiment="EXISTENCE:genetic interaction:GO:0030234
enzyme regulator activity [PMID:8063750]"
/experiment="EXISTENCE:genetic interaction:GO:0032781
positive regulation of ATP-dependent activity
[PMID:8063750]"
/note="Proteolipid associated with plasma membrane
H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase
activity; protein abundance increases in response to DNA
replication stress; PMP2 has a paralog, PMP1, that arose
from the whole genome duplication"
/codon_start=1
/product="proteolipid ATPase"
/protein_id="NP_010898.1"
/db_xref="GeneID:856697"
/db_xref="SGD:S000002103"
/translation="MLMSTLPGGVILVFILVGLACIAIISTIIYRKWQARQRGLQRF"
gene <123657..>124670
/gene="GTT3"
/locus_tag="YEL017W"
/db_xref="GeneID:856698"
mRNA <123657..>124670
/gene="GTT3"
/locus_tag="YEL017W"
/product="Gtt3p"
/transcript_id="NM_001178832.3"
/db_xref="GeneID:856698"
CDS 123657..124670
/gene="GTT3"
/locus_tag="YEL017W"
/experiment="EXISTENCE:direct assay:GO:0034399 nuclear
periphery [PMID:26928762]"
/note="hypothetical protein may be involved in glutathione
metabolism; function suggested by computational analysis
of large-scale protein-protein interaction data; N- and
C-terminal fusion proteins localize to the cell periphery"
/codon_start=1
/product="Gtt3p"
/protein_id="NP_010899.3"
/db_xref="GeneID:856698"
/db_xref="SGD:S000000743"
/translation="MPTKSTFSRWKKADLIDLANKLEIDGFPNYAKKSDMIDYLESHL
NHLEKPVDFKDDYPELRSFYESMTVDQSKDERNEYGSGSGNGSGSGSCDTATNDSDLE
KAYIKEDDDEKPQSGDETSATKPLSSRNANSNAKTNFNLLDFSTDNDSSTSAFTKFKF
NFQEYLSDIRYQTQKLNENVQDYLSTISAVDTIFSLLEFSFLVRNILAAGQPTSSSSL
ASSLEAAVAAHNKYQYTLDFCLPILTWLLFFRGIPTLVSYYINFIRYDLNIELDPMTF
NLTKFLISLAIFKTCNNKNIDFHSFRCVNQLWTQLCTVNRSLGMVPLVFSMVSCLLTL
YVL"
gene complement(<124737..>126218)
/gene="NPP2"
/locus_tag="YEL016C"
/db_xref="GeneID:856699"
mRNA complement(<124737..>126218)
/gene="NPP2"
/locus_tag="YEL016C"
/product="nucleotide diphosphatase/phosphodiesterase NPP2"
/transcript_id="NM_001178831.1"
/db_xref="GeneID:856699"
CDS complement(124737..126218)
/gene="NPP2"
/locus_tag="YEL016C"
/EC_number="3.1.4.1"
/EC_number="3.6.1.9"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0047429 nucleoside
triphosphate diphosphatase activity [PMID:16278456]"
/experiment="EXISTENCE:genetic interaction:GO:0017111
ribonucleoside triphosphate phosphatase activity
[PMID:16278456]"
/experiment="EXISTENCE:genetic interaction:GO:0047429
nucleoside triphosphate diphosphatase activity
[PMID:16278456]"
/experiment="EXISTENCE:mutant phenotype:GO:0009141
nucleoside triphosphate metabolic process [PMID:16278456]"
/experiment="EXISTENCE:mutant phenotype:GO:0017111
ribonucleoside triphosphate phosphatase activity
[PMID:16278456]"
/experiment="EXISTENCE:mutant phenotype:GO:0047429
nucleoside triphosphate diphosphatase activity
[PMID:16278456]"
/note="Nucleotide pyrophosphatase/phosphodiesterase;
mediates extracellular nucleotide phosphate hydrolysis
along with Npp1p and Pho5p; activity and expression
enhanced during conditions of phosphate starvation;
involved in spore wall assembly; SWAT-GFP and mCherry
fusion proteins localize to the endoplasmic reticulum;
NPP2 has a paralog, NPP1, that arose from the whole genome
duplication; npp1 npp2 double mutant exhibits reduced
dityrosine fluorescence relative to single mutants"
/codon_start=1
/product="nucleotide diphosphatase/phosphodiesterase NPP2"
/protein_id="NP_010900.1"
/db_xref="GeneID:856699"
/db_xref="SGD:S000000742"
/translation="MLLFEQPVDLEKNNEDDTNIKPFAISRHFLLKLLLCGIILIELL
LYSKCPKPIDNGPRTIANRSNTYFNGTHDFKTLTILISIDGFHPRLIDAKYTPFLYNL
HNLRSPYDMNITTAPYMIPSFPTQTFPNHWSMVTGKYPIEHGIVSNIFWDNFTSSEFR
PNNLDARIWSNTADPIWQLLQTESQGEYKVATHMWPGSEVVYEDHGDVPRERMPFYFG
KFNQWEKLQDKLAQIFRYIDMPQLKDRPELVISYIPNVDSYGHSFGYDLRDKRLQKLI
GEVDGFFLDLIEGLQKRNLLKISNVMIVSDHGMSNVNANDGEHVVVWERVFPADAMSA
FISHLYNEGPMMMVCLKNPRDKQWICDLIEAQLEKAYGDEISRKFHVILKEDFDPSWK
YFQYDNRKHRYDDRVGDIWILADEYYAIVKEMGDVPIGIMGTHGYNFNNCSDMASIFI
GMGPMFNNEVVPPFENIEVYNMLIKASALLGEEKTKKEKSLLQ"
gene <126629..>128284
/gene="EDC3"
/locus_tag="YEL015W"
/gene_synonym="DCP3; LSM16"
/db_xref="GeneID:856700"
mRNA <126629..>128284
/gene="EDC3"
/locus_tag="YEL015W"
/gene_synonym="DCP3; LSM16"
/product="Edc3p"
/transcript_id="NM_001178830.1"
/db_xref="GeneID:856700"
CDS 126629..128284
/gene="EDC3"
/locus_tag="YEL015W"
/gene_synonym="DCP3; LSM16"
/experiment="EXISTENCE:direct assay:GO:0000932 P-body
[PMID:15020463]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:24492965]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:23706738]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:23706738|PMID:22842922]"
/experiment="EXISTENCE:genetic interaction:GO:0033962
P-body assembly [PMID:18981231]"
/experiment="EXISTENCE:genetic interaction:GO:0042149
cellular response to glucose starvation [PMID:18981231]"
/experiment="EXISTENCE:mutant phenotype:GO:0031087
deadenylation-independent decapping of nuclear-transcribed
mRNA [PMID:15225544]"
/experiment="EXISTENCE:mutant phenotype:GO:0033962 P-body
assembly [PMID:18981231|PMID:17984320]"
/experiment="EXISTENCE:mutant phenotype:GO:0042149
cellular response to glucose starvation [PMID:18981231]"
/experiment="EXISTENCE:mutant phenotype:GO:1900153
positive regulation of nuclear-transcribed mRNA catabolic
process, deadenylation-dependent decay
[PMID:24492965|PMID:23956223]"
/note="Non-essential conserved protein with a role in mRNA
decapping; specifically affects the function of the
decapping enzyme Dcp1p; mediates decay of the RPS28B mRNA
via binding to both Rps28Bp (or Rps28Ap) and the RPS28B
mRNA; mediates decay of the YRA1 mRNA by a different,
translation-independent mechanism; localizes to
cytoplasmic mRNA processing bodies; forms cytoplasmic foci
upon DNA replication stress"
/codon_start=1
/product="Edc3p"
/protein_id="NP_010901.1"
/db_xref="GeneID:856700"
/db_xref="SGD:S000000741"
/translation="MSQFVGFGVQVELKDGKLIQGKIAKATSKGLTLNDVQFGDGGKS
QAFKVRASRLKDLKVLTVASQSGKRKQQRQQQQQNDYNQNRGEHIDWQDDDVSKIKQQ
EDFDFQRNLGMFNKKDVFAQLKQNDDILPENRLQGHNRKQTQLQQNNYQNDELVIPDA
KKDSWNKISSRNEQSTHQSQPQQDAQDDLVLEDDEHEYDVDDIDDPKYLPITQSLNIT
HLIHSATNSPSINDKTKGTVINDKDQVLAKLGQMIISQSRSNSTSLPAANKQTTIRSK
NTKQNIPMATPVQLLEMESITSEFFSINSAGLLENFAVNASFFLKQKLGGRARLRLQN
SNPEPLVVILASDSNRSGAKALALGRHLCQTGHIRVITLFTCSQNELQDSMVKKQTDI
YKKCGGKIVNSVSSLESAMETLNSPVEIVIDAMQGYDCTLSDLAGTSEVIESRIKSMI
SWCNKQRGSTKVWSLDIPNGFDAGSGMPDIFFSDRIEATGIICSGWPLIAINNLIANL
PSLEDAVLIDIGIPQGAYSQRTSLRKFQNCDLFVTDGSLLLDL"
gene complement(<128303..>128608)
/locus_tag="YEL014C"
/db_xref="GeneID:856701"
mRNA complement(<128303..>128608)
/locus_tag="YEL014C"
/product="uncharacterized protein"
/transcript_id="NM_001348821.1"
/db_xref="GeneID:856701"
CDS complement(128303..128608)
/locus_tag="YEL014C"
/note="hypothetical protein; conserved among S. cerevisiae
strains"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335765.1"
/db_xref="GeneID:856701"
/db_xref="SGD:S000000740"
/translation="MTALFCLELRTNIFLIMNDCIIINYWKGFIFSFHSYFFPFRFES
SLRAHYPGKRNYSDFSVIPLPYYIDVRSFHICESQHIIALPLQIPLPYRMLIRMYPV"
gene <128825..>130561
/gene="VAC8"
/locus_tag="YEL013W"
/gene_synonym="YEB3"
/db_xref="GeneID:856702"
mRNA <128825..>130561
/gene="VAC8"
/locus_tag="YEL013W"
/gene_synonym="YEB3"
/product="protein anchor VAC8"
/transcript_id="NM_001178828.3"
/db_xref="GeneID:856702"
CDS 128825..130561
/gene="VAC8"
/locus_tag="YEL013W"
/gene_synonym="YEB3"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane
[PMID:9739084|PMID:9490720|PMID:9664035|PMID:16301533|PMID
:26928762]"
/experiment="EXISTENCE:direct assay:GO:0000425 pexophagy
[PMID:31262095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0006914 autophagy
[PMID:31262095]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:10888680]"
/experiment="EXISTENCE:direct assay:GO:0031965 nuclear
membrane [PMID:10888680]"
/experiment="EXISTENCE:direct assay:GO:0034517 ribophagy
[PMID:31262095]"
/experiment="EXISTENCE:direct assay:GO:0045121 membrane
raft [PMID:10888680]"
/experiment="EXISTENCE:direct assay:GO:0071561
nucleus-vacuole junction [PMID:10888680]"
/experiment="EXISTENCE:direct assay:GO:0071563
Myo2p-Vac17p-Vac8p transport complex [PMID:12529432]"
/experiment="EXISTENCE:mutant phenotype:GO:0000011 vacuole
inheritance [PMID:10888680|PMID:8885233|PMID:16301533]"
/experiment="EXISTENCE:mutant phenotype:GO:0000045
autophagosome assembly [PMID:31649143]"
/experiment="EXISTENCE:mutant phenotype:GO:0000407
phagophore assembly site [PMID:31649143]"
/experiment="EXISTENCE:mutant phenotype:GO:0016236
macroautophagy [PMID:15901835]"
/experiment="EXISTENCE:mutant phenotype:GO:0031503
protein-containing complex localization [PMID:34893607]"
/experiment="EXISTENCE:mutant phenotype:GO:0034497 protein
localization to phagophore assembly site
[PMID:32508216|PMID:34893607]"
/experiment="EXISTENCE:mutant phenotype:GO:0034727
piecemeal microautophagy of the nucleus
[PMID:18701704|PMID:12529432]"
/experiment="EXISTENCE:mutant phenotype:GO:0042144 vacuole
fusion, non-autophagic [PMID:16301533|PMID:11441010]"
/experiment="EXISTENCE:mutant phenotype:GO:0043495
protein-membrane adaptor activity [PMID:12594460]"
/experiment="EXISTENCE:mutant phenotype:GO:0051656
establishment of organelle localization [PMID:32508216]"
/experiment="EXISTENCE:mutant phenotype:GO:0061724
lipophagy [PMID:38354739]"
/experiment="EXISTENCE:mutant phenotype:GO:0071255 Cvt
vesicle assembly [PMID:10837477]"
/experiment="EXISTENCE:mutant phenotype:GO:0071562
nucleus-vacuole junction assembly [PMID:10888680]"
/experiment="EXISTENCE:mutant phenotype:GO:1903044 protein
localization to membrane raft [PMID:10888680]"
/experiment="EXISTENCE:physical interaction:GO:0043495
protein-membrane adaptor activity [PMID:12594460]"
/experiment="EXISTENCE:physical interaction:GO:0071561
nucleus-vacuole junction [PMID:10888680]"
/experiment="EXISTENCE:physical interaction:GO:0071563
Myo2p-Vac17p-Vac8p transport complex
[PMID:12594460|PMID:12529432]"
/note="Vacuole-specific Myo2p receptor; Myo2p-Vac17p-Vac8p
transport complex subunit required for vacuolar
inheritance; mediates anchoring of phosphatidylinositol
3-kinase complex I (PIK3C3-C1) to autophagosome; required
with Atg13p for the vesicle closure step of the
cytoplasm-to-vacuole (CVT) pathway, for homotypic
vacuole-vacuole fusion and for nucleus-vacuole junction
formation with Nvj1p; contains 11 armadillo (ARM) repeats;
myristoylated, palmitoylated, and phosphorylated"
/codon_start=1
/product="protein anchor VAC8"
/protein_id="NP_010903.3"
/db_xref="GeneID:856702"
/db_xref="SGD:S000000739"
/translation="MGSCCSCLKDSSDEASVSPIADNEREAVTLLLGYLEDKDQLDFY
SGGPLKALTTLVYSDNLNLQRSAALAFAEITEKYVRQVSREVLEPILILLQSQDPQIQ
VAACAALGNLAVNNENKLLIVEMGGLEPLINQMMGDNVEVQCNAVGCITNLATRDDNK
HKIATSGALIPLTKLAKSKHIRVQRNATGALLNMTHSEENRKELVNAGAVPVLVSLLS
STDPDVQYYCTTALSNIAVDEANRKKLAQTEPRLVSKLVSLMDSPSSRVKCQATLALR
NLASDTSYQLEIVRAGGLPHLVKLIQSDSIPLVLASVACIRNISIHPLNEGLIVDAGF
LKPLVRLLDYKDSEEIQCHAVSTLRNLAASSEKNRKEFFESGAVEKCKELALDSPVSV
QSEISACFAILALADVSKLDLLEANILDALIPMTFSQNQEVSGNAAAALANLCSRVNN
YTKIIEAWDRPNEGIRGFLIRFLKSDYATFEHIALWTILQLLESHNDKVEDLVKNDDD
IINGVRKMADVTFERLQRSGIDVKNPGSNNNPSSNDNNSNNNDTGSEHQPVEDASLEL
YNITQQILQFLH"
gene complement(131082..131153)
/locus_tag="YNCE0008C"
/db_xref="GeneID:856703"
tRNA complement(131082..131153)
/locus_tag="YNCE0008C"
/product="tRNA-Gln"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:15706032]"
/note="Glutamine tRNA (tRNA-Gln), predicted by tRNAscan-SE
analysis; thiolation of uridine at wobble position (34)
requires Ncs6p"
/db_xref="GeneID:856703"
/db_xref="SGD:S000006697"
gene <131772..>132551
/gene="UBC8"
/locus_tag="YEL012W"
/gene_synonym="GID3"
/db_xref="GeneID:856704"
mRNA join(<131772..131776,131900..>132551)
/gene="UBC8"
/locus_tag="YEL012W"
/gene_synonym="GID3"
/product="E2 ubiquitin-conjugating protein UBC8"
/transcript_id="NM_001178827.1"
/db_xref="GeneID:856704"
CDS join(131772..131776,131900..132551)
/gene="UBC8"
/locus_tag="YEL012W"
/gene_synonym="GID3"
/EC_number="2.3.2.23"
/experiment="EXISTENCE:direct assay:GO:0004842
ubiquitin-protein transferase activity [PMID:1869573]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:10811607]"
/experiment="EXISTENCE:mutant phenotype:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:10811607]"
/experiment="EXISTENCE:mutant phenotype:GO:0043161
proteasome-mediated ubiquitin-dependent protein catabolic
process [PMID:12686616]"
/experiment="EXISTENCE:mutant phenotype:GO:0045721
negative regulation of gluconeogenesis [PMID:12686616]"
/experiment="EXISTENCE:mutant phenotype:GO:0070096
mitochondrial outer membrane translocase complex assembly
[PMID:36253107]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:29519818]"
/note="Ubiquitin-conjugating enzyme that regulates
gluconeogenesis; negatively regulates gluconeogenesis by
mediating the glucose-induced ubiquitination of
fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme
that catalyzes the ubiquitination of histones in vitro"
/codon_start=1
/product="E2 ubiquitin-conjugating protein UBC8"
/protein_id="NP_010904.2"
/db_xref="GeneID:856704"
/db_xref="SGD:S000000738"
/translation="MSSSKRRIETDVMKLLMSDHQVDLINDSMQEFHVKFLGPKDTPY
ENGVWRLHVELPDNYPYKSPSIGFVNKIFHPNIDIASGSICLDVINSTWSPLYDLINI
VEWMIPGLLKEPNGSDPLNNEAATLQLRDKKLYEEKIKEYIDKYATKEKYQQMFGGDN
DSDDSDSGGDLQEEDSDSDEDMDGTGVSSGDDSVDELSEDLSDIDVSDDDDYDEVANQ
"
gene <133120..>135234
/gene="GLC3"
/locus_tag="YEL011W"
/gene_synonym="GHA1"
/db_xref="GeneID:856705"
mRNA <133120..>135234
/gene="GLC3"
/locus_tag="YEL011W"
/gene_synonym="GHA1"
/product="1,4-alpha-glucan branching enzyme"
/transcript_id="NM_001178826.1"
/db_xref="GeneID:856705"
CDS 133120..135234
/gene="GLC3"
/locus_tag="YEL011W"
/gene_synonym="GHA1"
/EC_number="2.4.1.18"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:22842922]"
/experiment="EXISTENCE:mutant phenotype:GO:0003844
1,4-alpha-glucan branching enzyme activity [PMID:1729600]"
/experiment="EXISTENCE:mutant phenotype:GO:0005978
glycogen biosynthetic process [PMID:1729600]"
/note="Glycogen branching enzyme, involved in glycogen
accumulation; green fluorescent protein (GFP)-fusion
protein localizes to the cytoplasm in a punctate pattern;
relocalizes from nucleus to cytoplasmic foci upon DNA
replication stress; glycogen accumulation defect of the
null mutant is functionally complemented by human GBE1,
which is associated with glycogen storage disease"
/codon_start=1
/product="1,4-alpha-glucan branching enzyme"
/protein_id="NP_010905.1"
/db_xref="GeneID:856705"
/db_xref="SGD:S000000737"
/translation="MYNIPDNVKGAVEFDPWLKPFADVLSERRYLADKWLYDITHATP
DGSYQSLSKFARDSYKSYGLHANPETKEITYKEWAPNAERAFLVGDFNNWDTTSHELK
NKDEFGNFTITLHPLPNGDFAIPHDSKIKVMFILPDGSKIFRLPAWITRATQPSKETS
KQFGPAYEGRFWNPENPYKFVHPRPKFSESVDSLRIYEAHVGISSPEPKITTYKEFTE
KVLPRIKYLGYDAIQLMAIMEHAYYASFGYQVTNFFAASSRFGTPEELKELIDTAHSM
GILVLLDVVHSHASKNVEDGLNMFDGSDHQYFHSISSGRGEHPLWDSRLFNYGKFEVQ
RFLLANLAFYVDVYQFDGFRFDGVTSMLYVHHGVGAGGSFSGDYNEYLSRDRSFVDHE
ALAYLMLANDLVHEMLPNLAVTVAEDVSGYPTLCLPRSIGGTGFDYRLAMALPDMWIK
LIKEKKDDEWEMGSIVYTLTNRRYGEKVVAYCESHDQALVGDKTLAFWLMDAAMYTDM
TVLKEPSIVIDRGIALHKMIRLITHSLGGEAYLNFEGNEFGHPEWLDFPNVNNGDSYK
YARRQFNLADDPLLRYQNLNEFDRSMQLCEKRHKWLNTKQAYVSLKHEGDKMIVFERN
NLLFIFNFHPTNSYSDYRVGVEKAGTYHIVLNSDRAEFGGHNRINESSEFFTTDLEWN
NRKNFLQVYIPSRVALVLALKE"
gene complement(135425..135497)
/locus_tag="YNCE0009C"
/db_xref="GeneID:856706"
tRNA complement(135425..135497)
/locus_tag="YNCE0009C"
/product="tRNA-Lys"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:8915539]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:8915539]"
/experiment="EXISTENCE:mutant phenotype:GO:0030533 triplet
codon-amino acid adaptor activity [PMID:17560369]"
/experiment="EXISTENCE:mutant phenotype:GO:0032543
mitochondrial translation [PMID:17560369]"
/note="Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE
analysis; a small portion is imported into mitochondria
via interaction with mt lysyl-tRNA synthetase Msk1p and is
necessary to decode AAG codons at high temperature, when
base modification of mt-encoded tRNA-Lys is reduced"
/db_xref="GeneID:856706"
/db_xref="SGD:S000006618"
repeat_region complement(135612..135939)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006887"
gene complement(<136371..>136778)
/locus_tag="YEL009C-A"
/db_xref="GeneID:31009438"
mRNA complement(<136371..>136778)
/locus_tag="YEL009C-A"
/product="uncharacterized protein"
/transcript_id="NM_001348822.1"
/db_xref="GeneID:31009438"
CDS complement(136371..136778)
/locus_tag="YEL009C-A"
/note="hypothetical protein; conserved among S. cerevisiae
strains; overlaps ORF YEL010W"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335766.1"
/db_xref="GeneID:31009438"
/db_xref="SGD:S000028741"
/translation="MNTLLKKYRKQRYAWLRFLLFSKIEGSLPVALRILLSLQPFCCN
IYRKYYQENKKVKSTSGNTALKIIEKLESLVSNLRLKYSQMFSLLFLHSYNCLQSLAT
ETFRIIKKREKNLLSLLIPGSCIEIARHFVLKE"
repeat_region 137324..137639
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006891"
repeat_region 138221..138553
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006892"
gene 138666..138737
/locus_tag="YNCE0010W"
/db_xref="GeneID:856708"
tRNA 138666..138737
/locus_tag="YNCE0010W"
/product="tRNA-Arg"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE
analysis; one of 11 nuclear tRNA genes containing the
tDNA-anticodon UCU (converted to mcm5-UCU in the mature
tRNA), decodes AGA codons into arginine, one of 19 nuclear
tRNAs for arginine"
/db_xref="GeneID:856708"
/db_xref="SGD:S000006710"
gene complement(<138918..>139763)
/gene="GCN4"
/locus_tag="YEL009C"
/gene_synonym="AAS101; AAS3; ARG9"
/db_xref="GeneID:856709"
mRNA complement(<138918..>139763)
/gene="GCN4"
/locus_tag="YEL009C"
/gene_synonym="AAS101; AAS3; ARG9"
/product="amino acid starvation-responsive transcription
factor GCN4"
/transcript_id="NM_001178824.3"
/db_xref="GeneID:856709"
CDS complement(138918..139763)
/gene="GCN4"
/locus_tag="YEL009C"
/gene_synonym="AAS101; AAS3; ARG9"
/experiment="EXISTENCE:direct assay:GO:0001228 DNA-binding
transcription activator activity, RNA polymerase
II-specific [PMID:19940160]"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:18794364]"
/experiment="EXISTENCE:direct assay:GO:0003700 DNA-binding
transcription factor activity [PMID:16581788]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14648200|PMID:12455686]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding
[PMID:3530496|PMID:19158363|PMID:3907851|PMID:2204805|PMID
:3464968|PMID:3290651]"
/experiment="EXISTENCE:direct assay:GO:0045899 positive
regulation of RNA polymerase II transcription
preinitiation complex assembly [PMID:18794364]"
/experiment="EXISTENCE:genetic interaction:GO:0045899
positive regulation of RNA polymerase II transcription
preinitiation complex assembly [PMID:15121833]"
/experiment="EXISTENCE:genetic interaction:GO:0060261
positive regulation of transcription initiation by RNA
polymerase II [PMID:1425591]"
/experiment="EXISTENCE:genetic interaction:GO:1903833
positive regulation of cellular response to amino acid
starvation [PMID:6351059]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:15590823|PMID:26979516]"
/experiment="EXISTENCE:mutant phenotype:GO:0001228
DNA-binding transcription activator activity, RNA
polymerase II-specific [PMID:19940160|PMID:16581788]"
/experiment="EXISTENCE:mutant phenotype:GO:0003700
DNA-binding transcription factor activity [PMID:16581788]"
/experiment="EXISTENCE:mutant phenotype:GO:0010688
negative regulation of ribosomal protein gene
transcription by RNA polymerase II [PMID:21183953]"
/experiment="EXISTENCE:mutant phenotype:GO:0031669
cellular response to nutrient levels [PMID:21183953]"
/experiment="EXISTENCE:mutant phenotype:GO:0045899
positive regulation of RNA polymerase II transcription
preinitiation complex assembly [PMID:15121833]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:21148207]"
/experiment="EXISTENCE:mutant phenotype:GO:0060261
positive regulation of transcription initiation by RNA
polymerase II [PMID:1425591]"
/experiment="EXISTENCE:mutant phenotype:GO:1903833
positive regulation of cellular response to amino acid
starvation [PMID:6351059]"
/experiment="EXISTENCE:mutant phenotype:GO:1990139 protein
localization to nuclear periphery [PMID:31211995]"
/experiment="EXISTENCE:physical interaction:GO:0001228
DNA-binding transcription activator activity, RNA
polymerase II-specific [PMID:19940160]"
/experiment="EXISTENCE:physical interaction:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:20308326|PMID:21183953]"
/note="bZIP transcriptional activator of amino acid
biosynthetic genes; activator responds to amino acid
starvation; expression is tightly regulated at both the
transcriptional and translational levels; contains four
upstream open reading frames (uORFs) in 5' untranslated
region which regulate translation; participates in sulfur
starvation-induced autophagy along with Met4p, but is
dispensable"
/codon_start=1
/product="amino acid starvation-responsive transcription
factor GCN4"
/protein_id="NP_010907.3"
/db_xref="GeneID:856709"
/db_xref="SGD:S000000735"
/translation="MSEYQPSLFALNPMGFSPLDGSKSTNENVSASTSTAKPMVGQLI
FDKFIKTEEDPIIKQDTPSNLDFDFALPQTATAPDAKTVLPIPELDDAVVESFFSSST
DSTPMFEYENLEDNSKEWTSLFDNDIPVTTDDVSLADKAIESTEEVSLVPSNLEVSTT
SFLPTPVLEDAKLTQTRKVKKPNSVVKKSHHVGKDDESRLDHLGVVAYNRKQRSIPLS
PIVPESSDPAALKRARNTEAARRSRARKLQRMKQLEDKVEELLSKNYHLENEVARLKK
LVGER"
regulatory complement(139903..139914)
/regulatory_class="other"
/note="One of four upstream open reading frames (uORFs) in
5' untranslated region of GCN4 gene, regulate translation"
regulatory complement(139928..139939)
/regulatory_class="other"
/note="One of four upstream open reading frames (uORFs) in
5' untranslated region of GCN4 gene, regulate translation"
regulatory complement(140048..140056)
/regulatory_class="other"
/note="One of four upstream open reading frames (uORFs) in
5' untranslated region of GCN4 gene, regulate translation"
regulatory complement(140113..140124)
/regulatory_class="other"
/note="One of four upstream open reading frames (uORFs) in
5' untranslated region of GCN4 gene, regulate translation"
gene <140512..>140892
/locus_tag="YEL008W"
/db_xref="GeneID:856710"
mRNA <140512..>140892
/locus_tag="YEL008W"
/product="uncharacterized protein"
/transcript_id="NM_001348823.1"
/db_xref="GeneID:856710"
CDS 140512..140892
/locus_tag="YEL008W"
/note="hypothetical protein; conserved among S. cerevisiae
strains; YEL008W is not an essential gene; predicted to be
involved in metabolism"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335767.1"
/db_xref="GeneID:856710"
/db_xref="SGD:S000000734"
/translation="MLMHFIPRRSSKAVQFNWLEIGVWRGGVYVKKGIVNARTNNSNF
YKMPVVFGIVKGMGALCVHAWGMGYGGIIDCSPVTEHGNISVLQLEYMPWICLSKIMS
CSVASRHSNVWIMDETIAPLVGCL"
gene <141892..>143892
/gene="MIT1"
/locus_tag="YEL007W"
/db_xref="GeneID:856711"
mRNA <141892..>143892
/gene="MIT1"
/locus_tag="YEL007W"
/product="Mit1p"
/transcript_id="NM_001178822.1"
/db_xref="GeneID:856711"
CDS 141892..143892
/gene="MIT1"
/locus_tag="YEL007W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="Transcriptional regulator of pseudohyphal growth;
protein with sequence similarity to S. pombe gti1+
(gluconate transport inducer 1) and C. albicans Wor1"
/codon_start=1
/product="Mit1p"
/protein_id="NP_010909.1"
/db_xref="GeneID:856711"
/db_xref="SGD:S000000733"
/translation="MDIEPTFKGYIEDEDDALLILQATLDGKLKHIPRRPYEIERPYL
IVSGSIFVFIEEISGIKRWTDGVSWSPSRISGKFLIYKELDKENAGSNANATSSGSTD
SAVITDGTSGARNNPSSSKIKLPPLKNHQFDLPPTMGHSSFESEQDTSISPSNRSNLP
LKYTGLVKKTISVKLKRPPFNSIENLHIVSYYSVKDIKQNCLVTPKASPFLKDVRPSQ
ELIVAMGNTTLGNVKNNSTTTGNGPNNINNKSNSSTPLNTVISTNNNSANINAAGSNQ
FTSANKNYYYKNDESSGYPITQFAPALPSTTLMYTANPPYITQSPDNTNATGMNTHVN
NNNNNSNNSSNSNNSNNNNNNNNNNNNNNNNNINNINNVNTNAGNGNNPNRFHNASFA
YNTTGDFINPQQQGQISYPFYYTTIPINNPNYYTTQPPNPVTNASTNENQGYSTSSTQ
HPYYGHPTESQSASAAAGATGTPGTAENVLPVSSMQPLLHQANNNSASSATSTAPYPV
YSMNVNVPYYNSSASAYKRAQENTTSNTNAEPSGATSTNSGTMLSNPAYANSQYTPSQ
VYYQGFPQYAMASAQNPSMYQHQHQHPLPTVYPIATPQQNIMSSGHTLSTIGSDPQHH
HYQQEPNDHKNFAMGHANNNILNITNNDTMNNLNTNTSTTTQ"
gene <144327..>145334
/gene="YEA6"
/locus_tag="YEL006W"
/gene_synonym="NDT2"
/db_xref="GeneID:856712"
mRNA <144327..>145334
/gene="YEA6"
/locus_tag="YEL006W"
/gene_synonym="NDT2"
/product="NAD+ transporter"
/transcript_id="NM_001178821.1"
/db_xref="GeneID:856712"
CDS 144327..145334
/gene="YEA6"
/locus_tag="YEL006W"
/gene_synonym="NDT2"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0035352 NAD
transmembrane transport [PMID:16291748]"
/experiment="EXISTENCE:genetic interaction:GO:0051724 NAD
transmembrane transporter activity [PMID:16291748]"
/experiment="EXISTENCE:mutant phenotype:GO:0035352 NAD
transmembrane transport [PMID:16291748]"
/experiment="EXISTENCE:mutant phenotype:GO:0051724 NAD
transmembrane transporter activity [PMID:16291748]"
/note="Mitochondrial NAD+ transporter; member of the
mitochondrial carrier subfamily (see also YIA6); has
putative human ortholog; YEA6 has a paralog, YIA6, that
arose from the whole genome duplication; human NAD+
transporter MCART1/SLC25A51 functionally complements the
yia6 yea6 double null mutant, and yeast YEA6 reciprocally
complements defects in MCART1/SLC25A51 null cells"
/codon_start=1
/product="NAD+ transporter"
/protein_id="NP_010910.1"
/db_xref="GeneID:856712"
/db_xref="SGD:S000000732"
/translation="MNNGDNKTTLENSKNASLANGNYAIPTKLNRLKKNADPRVAAIS
GALSGALSAMLVCPFDVAKTRLQAQGLQNMTHQSQHYKGFFGTFATIFKDEGAAGLYK
GLQPTVLGYIPTLMIYFSVYDFCRKYSVDIFPHSPFLSNASSAITAGAISTVATNPIW
VVKTRLMLQTGIGKYSTHYKGTIDTFRKIIQQEGAKALYAGLVPALLGMLNVAIQFPL
YENLKIRFGYSESTDVSTDVTSSNFQKLILASMLSKMVASTVTYPHEILRTRMQLKSD
LPNTVQRHLLPLIKITYRQEGFAGFYSGFATNLVRTVPAAVVTLVSFEYSKKYLTTFF
Q"
rep_origin 145678..145840
/note="ARS510; Autonomously Replicating Sequence"
/db_xref="SGD:S000077382"
gene complement(<145907..>146755)
/gene="VAB2"
/locus_tag="YEL005C"
/gene_synonym="VAB31"
/db_xref="GeneID:856713"
mRNA complement(<145907..>146755)
/gene="VAB2"
/locus_tag="YEL005C"
/gene_synonym="VAB31"
/product="Vab2p"
/transcript_id="NM_001178820.3"
/db_xref="GeneID:856713"
CDS complement(145907..146755)
/gene="VAB2"
/locus_tag="YEL005C"
/gene_synonym="VAB31"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0031083 BLOC-1
complex [PMID:23547030]"
/experiment="EXISTENCE:mutant phenotype:GO:0007032
endosome organization [PMID:23547030]"
/experiment="EXISTENCE:mutant phenotype:GO:0032880
regulation of protein localization [PMID:23547030]"
/note="Subunit of the BLOC-1 complex involved in endosomal
maturation; interacts with Vps21p-GFP; has potential role
in vacuolar function, as suggested by its ability to bind
Vac8p; likely member of; Vab2p-GFP-fusion localizes to
cytoplasm in punctate pattern"
/codon_start=1
/product="Vab2p"
/protein_id="NP_010911.3"
/db_xref="GeneID:856713"
/db_xref="SGD:S000000731"
/translation="MVADLTKGILKWKSRIEFDAVGSSSYYEELKGLPPLASHKKLTQ
AAIFNSTKYELLQVKKDILSIYEVVSRDIDEERNQMQQIELQLKKSLKKVEHSYKNVL
KQRASTNCINGNDRLLANAEKKIGSLNEELACVNDIVSDIVNNLTALNANLPKKAQLL
KDDSINVAHYPLLFDFLHKSCPKSIIATSEASIHENASPSPLLEHDELPAESINSFYG
ENELQSDSLAPLQTHDDNISSCKKILPPKFNTTSGPSIETNFENISADGLTYTKCSLK
NSISLT"
gene <146951..>147979
/gene="YEA4"
/locus_tag="YEL004W"
/db_xref="GeneID:856714"
mRNA <146951..>147979
/gene="YEA4"
/locus_tag="YEL004W"
/product="Yea4p"
/transcript_id="NM_001178819.1"
/db_xref="GeneID:856714"
CDS 146951..147979
/gene="YEA4"
/locus_tag="YEL004W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:10788474]"
/experiment="EXISTENCE:genetic interaction:GO:0015786
UDP-glucose transmembrane transport [PMID:18693752]"
/experiment="EXISTENCE:mutant phenotype:GO:0005462
UDP-N-acetylglucosamine transmembrane transporter activity
[PMID:10788474]"
/experiment="EXISTENCE:mutant phenotype:GO:0006031 chitin
biosynthetic process [PMID:10788474]"
/experiment="EXISTENCE:mutant phenotype:GO:0015786
UDP-glucose transmembrane transport [PMID:18693752]"
/experiment="EXISTENCE:mutant phenotype:GO:0055085
transmembrane transport [PMID:10788474]"
/experiment="EXISTENCE:mutant phenotype:GO:1990569
UDP-N-acetylglucosamine transmembrane transport
[PMID:10788474]"
/note="Uridine diphosphate-N-acetylglucosamine
(UDP-GlcNAc) transporter; required for cell wall chitin
synthesis; localized to the ER"
/codon_start=1
/product="Yea4p"
/protein_id="NP_010912.1"
/db_xref="GeneID:856714"
/db_xref="SGD:S000000730"
/translation="MWNSLKAFALVFGGCCSNVITFETLMSNETGSINNLITFCQFLF
VTCQGLPEFLDVHQPFPYFKPLKTPLHVYVITVVLFYISSTTNNNVFKYNISIPIHIV
FRCFGTVITMFTCWLLNGRKYTKIQILSTLFLTIGAIIASLFKDADFRYQDLKLQAWK
IGSDQSVDLTFIFGICILVLSSFTSSLLSAYNERTYQKYGKHWKENIFYSHFLSLPLF
LFSRKQLIHEYRVMRKSERILCSNFGGKILVPREETLLLFNVLTQYFCVKGVNILASK
TNALTLSITLLVRKFISLLLSVRLFDNNLSYTGYIGVYLVFFGAFIYSLGSIHPRQND
KGAIKKSK"
gene <148176..>148599
/gene="GIM4"
/locus_tag="YEL003W"
/gene_synonym="PFD2"
/db_xref="GeneID:856715"
mRNA join(<148176..148194,148283..>148599)
/gene="GIM4"
/locus_tag="YEL003W"
/gene_synonym="PFD2"
/product="tubulin-binding prefolding complex subunit GIM4"
/transcript_id="NM_001178818.1"
/db_xref="GeneID:856715"
CDS join(148176..148194,148283..148599)
/gene="GIM4"
/locus_tag="YEL003W"
/gene_synonym="PFD2"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:9463374]"
/experiment="EXISTENCE:direct assay:GO:0015631 tubulin
binding [PMID:9463374]"
/experiment="EXISTENCE:mutant phenotype:GO:0007021 tubulin
complex assembly [PMID:9463374]"
/experiment="EXISTENCE:physical interaction:GO:0016272
prefoldin complex [PMID:9463374]"
/note="Subunit of the heterohexameric cochaperone
prefoldin complex; complex binds specifically to cytosolic
chaperonin and transfers target proteins to it"
/codon_start=1
/product="tubulin-binding prefolding complex subunit GIM4"
/protein_id="NP_010913.2"
/db_xref="GeneID:856715"
/db_xref="SGD:S000000729"
/translation="MEQRNNVFQAKYNEYKQILEELQTKIIELGHDKDEHTIVIKTLK
DAEPTRKCYRMIGGALVESDVQTSLPILETKKENIEGTISKMKETLIQTAKEFEKWKK
DNKIQVVKN"
gene complement(<148722..>150014)
/gene="WBP1"
/locus_tag="YEL002C"
/db_xref="GeneID:856716"
mRNA complement(<148722..>150014)
/gene="WBP1"
/locus_tag="YEL002C"
/product="dolichyl-diphosphooligosaccharide-protein
glycotransferase"
/transcript_id="NM_001178817.3"
/db_xref="GeneID:856716"
CDS complement(148722..150014)
/gene="WBP1"
/locus_tag="YEL002C"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:1724755]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:12860997|PMID:1724755]"
/experiment="EXISTENCE:direct assay:GO:0008250
oligosaccharyltransferase complex [PMID:10358084]"
/experiment="EXISTENCE:direct assay:GO:0016757
glycosyltransferase activity [PMID:1600939]"
/experiment="EXISTENCE:mutant phenotype:GO:0004576
oligosaccharyl transferase activity [PMID:1600939]"
/experiment="EXISTENCE:mutant phenotype:GO:0005789
endoplasmic reticulum membrane [PMID:12974639]"
/experiment="EXISTENCE:physical interaction:GO:0006487
protein N-linked glycosylation [PMID:9405463]"
/experiment="EXISTENCE:physical interaction:GO:0008250
oligosaccharyltransferase complex [PMID:9405463]"
/note="Non-catalytic beta subunit of the
oligosaccharyltransferase (OST) complex; the OST complex
catalyzes N-linked glycosylation of newly synthesized
proteins in the ER; forms a pocket with Ost2p involved in
binding terminal glucose units of the donor glycan; human
homolog DDOST can complement yeast growth defect during
down-regulation of the yeast gene"
/codon_start=1
/product="dolichyl-diphosphooligosaccharide-protein
glycotransferase"
/protein_id="NP_010914.3"
/db_xref="GeneID:856716"
/db_xref="SGD:S000000728"
/translation="MRTDWNFFFCILLQAIFVVGTQTSRTLVLYDQSTEPLEEYSVYL
KDLEQRNYKLEYLDINSTSTTVDLYDKEQRLFDNIIVFPTKGGKNLARQIPVKQLIKF
FENEGNILCMSSPGAVPNTIRLFLNELGIYPSPKGHVIRDYFSPSSEELVVSSNHLLN
KYVYNARKSEDFVFGESSAALLENREQIVPILNAPRTSFTESKGKCNSWTSGSQGFLV
VGFQNLNNARLVWIGSSDFLKNKNQDSNQEFAKELLKWTFNEKSVIKSVHAVHSHADG
TSYDEEPYKIKDKVIYSVGFSEWNGEEWLPHIADDIQFELRQVDPYYRLTLSPSGNDS
ETQYYTTGEFILPDRHGVFTFLTDYRKIGLSFTTDKDVKAIRHLANDEYPRSWEISNS
WVYISAICGVIVAWIFFVVSFVTTSSVGKKLETFKKTN"
gene complement(<150301..>150978)
/gene="IRC22"
/locus_tag="YEL001C"
/db_xref="GeneID:856717"
mRNA complement(<150301..>150978)
/gene="IRC22"
/locus_tag="YEL001C"
/product="Irc22p"
/transcript_id="NM_001178816.1"
/db_xref="GeneID:856717"
CDS complement(150301..150978)
/gene="IRC22"
/locus_tag="YEL001C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095|PMID:26928762|PMID:22842922]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion localizes to the ER; YEL001C is
non-essential; null mutant displays increased levels of
spontaneous Rad52p foci"
/codon_start=1
/product="Irc22p"
/protein_id="NP_010915.1"
/db_xref="GeneID:856717"
/db_xref="SGD:S000000727"
/translation="MRFSMLIGFNLLTALSSFCAAISANNSDNVEHEQEVAEAVAPPS
INIEVKYDVVGKESENHDSFLEFYAEDTATLAYNVTNWEDTNITIFGVNGTIVTYPHG
YPVADITGASIGPYEMEVNGTSKFGQDVTLNLPEGQYFLIPFLLASRFDEIVRIAAPP
TLFEIVSPPISFFNPQFLSVQVIFLAIIGGVSYYYMKSKTNQRPSKKSATVKKVDESW
LPETYKK"
centromere 151987..152104
/note="CEN5; Chromosome V centromere"
/db_xref="SGD:S000006467"
centromere 151987..151996
/note="CEN5_CDEI of CEN5"
centromere 151997..152079
/note="CEN5_CDEII of CEN5"
centromere 152080..152104
/note="CEN5_CDEIII of CEN5"
gene <153520..>155808
/gene="MNN1"
/locus_tag="YER001W"
/db_xref="GeneID:856718"
mRNA <153520..>155808
/gene="MNN1"
/locus_tag="YER001W"
/product="alpha-1,3-mannosyltransferase MNN1"
/transcript_id="NM_001178892.1"
/db_xref="GeneID:856718"
CDS 153520..155808
/gene="MNN1"
/locus_tag="YER001W"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:7962051]"
/experiment="EXISTENCE:direct assay:GO:0006493 protein
O-linked glycosylation [PMID:7962051]"
/experiment="EXISTENCE:mutant phenotype:GO:0000033
alpha-1,3-mannosyltransferase activity [PMID:8146181]"
/experiment="EXISTENCE:mutant phenotype:GO:0006491
N-glycan processing [PMID:8146181]"
/note="Alpha-1,3-mannosyltransferase; integral membrane
glycoprotein of Golgi complex, required for addition of
alpha1,3-mannose linkages to N-linked and O-linked
oligosaccharides; one of five S. cerevisiae proteins of
MNN1 family; targeted to vacuole via AP-3 pathway"
/codon_start=1
/product="alpha-1,3-mannosyltransferase MNN1"
/protein_id="NP_010916.1"
/db_xref="GeneID:856718"
/db_xref="SGD:S000000803"
/translation="MLALRRFILNQRSLRSCTIPILVGALIIILVLFQLVTHRNDALI
RSSNVNSTNKKTLKDADPKVLIEAFGSPEVDPVDTIPVSPLELVPFYDQSIDTKRSSS
WLINKKGYYKHFNELSLTDRCKFYFRTLYTLDDEWTNSVKKLEYSINDNEGVDEGKDA
NGNPMDEKSERLYRRKYDMFQAFERIRAYDRCFMQANPVNIQEIFPKSDKMSKERVQS
KLIKTLNATFPNYDPDNFKKYDQFEFEHKMFPFINNFTTETFHEMVPKITSPFGKVLE
QGFLPKFDHKTGKVQEYFKYEYDPSKTFWANWRDMSAKVAGRGIVLSLGSNQFPLAVK
FIASLRFEGNTLPIQVVYRGDELSQELVDKLIYAARSPDFKPVENNYDNSTNVPQEIW
FLDVSNTIHPKWRGDFGSYKSKWLVVLLNLLQEFVFLDIDAISYEKIDNYFKTTEYQK
TGTVFYRERALRENVNERCIARYETLLPRNLESKNFQNSLLIDPDHALNECDNTLTTE
EYIFKAFFHHRRQHQLEAGLFAVDKSKHTIPLVLAAMIHLAKNTAHCTHGDKENFWLG
FLAAGHTYALQGVYSGAIGDYVKKTDLNGKRQEAAVEICSGQIAHMSTDKKTLLWVNG
GGTFCKHDNAAKDDWKKDGDFKKFKDQFKTFEEMEKYYYITPISSKYVILPDPKSDDW
HRASAGACGGYIWCATHKTLLKPYSYNHRTTHGELITLDEEQRLHIDAVNTVWSHANK
DNTRSFTEEEIKELENSRHEQS"
gene <156803..>157498
/gene="NOP16"
/locus_tag="YER002W"
/db_xref="GeneID:856719"
mRNA <156803..>157498
/gene="NOP16"
/locus_tag="YER002W"
/product="Nop16p"
/transcript_id="NM_001178893.3"
/db_xref="GeneID:856719"
CDS 156803..157498
/gene="NOP16"
/locus_tag="YER002W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10684247|PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:10684247]"
/experiment="EXISTENCE:direct assay:GO:0030687
preribosome, large subunit precursor
[PMID:17443350|PMID:11583614]"
/experiment="EXISTENCE:mutant phenotype:GO:0042273
ribosomal large subunit biogenesis [PMID:11583614]"
/note="Constituent of 66S pre-ribosomal particles;
involved in 60S ribosomal subunit biogenesis"
/codon_start=1
/product="Nop16p"
/protein_id="NP_010917.3"
/db_xref="GeneID:856719"
/db_xref="SGD:S000000804"
/translation="MTSVRKRKMNRSSVGKATRRNKDKQRKINIQSNPIIAANWDYSL
TMAQNYKKLGLRAKLQTPAGGKEADLSKVVKRIPLTKPVLDEDEDEDEGEDEQNDYNA
ATVELDENEIPEGGARIQRDKNGDVVRVVYGKKKNFDADEDVNEIKARDTTEETEVVK
KLEELASRPVIRKERSQSEREEEWLEKLYKKHGDDYKKMFFDKKLNIYQQSEGDLKRR
LLRWKKRNGIASK"
gene complement(<157736..>159118)
/gene="PMI40"
/locus_tag="YER003C"
/db_xref="GeneID:856720"
mRNA complement(join(<157736..158994,159088..>159118))
/gene="PMI40"
/locus_tag="YER003C"
/product="mannose-6-phosphate isomerase PMI40"
/transcript_id="NM_001178894.1"
/db_xref="GeneID:856720"
CDS complement(join(157736..158994,159088..159118))
/gene="PMI40"
/locus_tag="YER003C"
/EC_number="5.3.1.8"
/experiment="EXISTENCE:direct assay:GO:0004476
mannose-6-phosphate isomerase activity [PMID:8011630]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0000032 cell
wall mannoprotein biosynthetic process [PMID:1377774]"
/experiment="EXISTENCE:mutant phenotype:GO:0009101
glycoprotein biosynthetic process [PMID:1377774]"
/experiment="EXISTENCE:mutant phenotype:GO:0009298
GDP-mannose biosynthetic process [PMID:1377774]"
/note="Mannose-6-phosphate isomerase; catalyzes the
interconversion of fructose-6-P and mannose-6-P; required
for early steps in protein mannosylation"
/codon_start=1
/product="mannose-6-phosphate isomerase PMI40"
/protein_id="NP_010918.1"
/db_xref="GeneID:856720"
/db_xref="SGD:S000000805"
/translation="MSNKLFRLDAGYQQYDWGKIGSSSAVAQFAAHSDPSVQIEQDKP
YAELWMGTHSKMPSYNHESKESLRDIISKNPSAMLGKDIIDKFHATNELPFLFKVLSI
EKVLSIQAHPDKALGKILHAQDPKNYPDDNHKPEMAIAVTDFEGFCGFKPLQEIADEL
KRIPELRNIVGEETSRNFIENIQPSAQKGSPEDEQNKKLLQAVFSRVMNASDDKIKIQ
ARSLVERSKNSPSDFNKPDLPELIQRLNKQFPDDVGLFCGCLLLNHCRLNAGEAIFLR
AKDPHAYISGDIMECMAASDNVVRAGFTPKFKDVKNLVSMLTYTYDPVEKQKMQPLKF
DRSSGNGKSVLYNPPIEEFAVLETTFDEKLGQRHFEGVDGPSILITTKGNGYIKADGQ
KLKAEPGFVFFIAPHLPVDLEAEDEAFTTYRAFVEPN"
gene <159580..>160275
/gene="FMP52"
/locus_tag="YER004W"
/db_xref="GeneID:856721"
mRNA <159580..>160275
/gene="FMP52"
/locus_tag="YER004W"
/product="Fmp52p"
/transcript_id="NM_001178895.1"
/db_xref="GeneID:856721"
CDS 159580..160275
/gene="FMP52"
/locus_tag="YER004W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:16407407]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095]"
/note="hypothetical protein; localized to the
mitochondrial outer membrane; induced by treatment with
8-methoxypsoralen and UVA irradiation"
/codon_start=1
/product="Fmp52p"
/protein_id="NP_010919.1"
/db_xref="GeneID:856721"
/db_xref="SGD:S000000806"
/translation="MNGLVLGATGLCGGGFLRHAQEAPQFSKVYAILRRELPFPATDK
VVAIVERDNSKWSQLITNEMNPQVLFTALATTRAAAGGLDKQYKIDHDLNLQLAQAAK
EKGCETIVLVSSAGAHPDSRFGYMKMKGEIERDVIALDFKHIIILRPGPLLGERTNSK
QSGFGGNLTAALGTRVYRSRFQRLLGYPVYGDEVGKVGVHLALNTSGKDKVQFVSSKD
ILDISASLEKIAT"
gene <160550..>162442
/gene="YND1"
/locus_tag="YER005W"
/gene_synonym="APY1; YEJ5"
/db_xref="GeneID:856722"
mRNA <160550..>162442
/gene="YND1"
/locus_tag="YER005W"
/gene_synonym="APY1; YEJ5"
/product="apyrase"
/transcript_id="NM_001178896.3"
/db_xref="GeneID:856722"
CDS 160550..162442
/gene="YND1"
/locus_tag="YER005W"
/gene_synonym="APY1; YEJ5"
/EC_number="3.6.1.5"
/experiment="EXISTENCE:direct assay:GO:0000139 Golgi
membrane [PMID:10551827]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0017110 nucleoside
diphosphate phosphatase activity [PMID:10409709]"
/experiment="EXISTENCE:direct assay:GO:0017111
ribonucleoside triphosphate phosphatase activity
[PMID:10409709]"
/experiment="EXISTENCE:mutant phenotype:GO:0009101
glycoprotein biosynthetic process [PMID:10409709]"
/note="Apyrase with wide substrate specificity; helps
prevent inhibition of glycosylation by hydrolyzing
nucleoside tri- and diphosphates that inhibit
glycotransferases; partially redundant with Gda1p;
mediates adenovirus E4orf4-induced toxicity"
/codon_start=1
/product="apyrase"
/protein_id="NP_010920.3"
/db_xref="GeneID:856722"
/db_xref="SGD:S000000807"
/translation="MLIENTNDRFGIVIDAGSSGSRIHVFKWQDTESLLHATNQDSQS
ILQSVPHIHQEKDWTFKLNPGLSSFEKKPQDAYKSHIKPLLDFAKNIIPESHWSSCPV
FIQATAGMRLLPQDIQSSILDGLCQGLKHPAEFLVEDCSAQIQVIDGETEGLYGWLGL
NYLYGHFNDYNPEVSDHFTFGFMDMGGASTQIAFAPHDSGEIARHRDDIATIFLRSVN
GDLQKWDVFVSTWLGFGANQARRRYLAQLINTLPENTNDYENDDFSTRNLNDPCMPRG
SSTDFEFKDTIFHIAGSGNYEQCTKSIYPLLLKNMPCDDEPCLFNGVHAPRIDFANDK
FIGTSEYWYTANDVFKLGGEYNFDKFSKSLREFCNSNWTQILANSDKGVYNSIPENFL
KDACFKGNWVLNILHEGFDMPRIDVDAENVNDRPLFQSVEKVEERELSWTLGRILLYA
SGSILAGNDDFMVGIAPSERRTKLTGKKFIPGKLLESDQLRKQSSSLSNKGFLMWFAI
ICCIFYLIFHRSHIIRRRFSGLYNITKDFKTGIRRRLKFLRRSDPFSRLEEGELGTDV
DGFKDVYRMKSSSMFDLGKSSATMQREHEPQRTASQSANLAPSNLRPAFSMADFSKFK
DSRLYD"
gene <162723..>164285
/gene="NUG1"
/locus_tag="YER006W"
/db_xref="GeneID:856723"
mRNA <162723..>164285
/gene="NUG1"
/locus_tag="YER006W"
/product="RNA-binding GTPase NUG1"
/transcript_id="NM_001178897.1"
/db_xref="GeneID:856723"
CDS 162723..164285
/gene="NUG1"
/locus_tag="YER006W"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:16803892]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0003924 GTPase
activity [PMID:16803892]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10684247|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:10684247|PMID:16803892]"
/experiment="EXISTENCE:genetic interaction:GO:0000055
ribosomal large subunit export from nucleus
[PMID:11583615]"
/experiment="EXISTENCE:genetic interaction:GO:0030687
preribosome, large subunit precursor [PMID:16803892]"
/experiment="EXISTENCE:mutant phenotype:GO:0000055
ribosomal large subunit export from nucleus
[PMID:11583615]"
/experiment="EXISTENCE:mutant phenotype:GO:0000463
maturation of LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12089522]"
/note="GTPase that associates with nuclear 60S
pre-ribosomes; required for export of 60S ribosomal
subunits from the nucleus"
/codon_start=1
/product="RNA-binding GTPase NUG1"
/protein_id="NP_010921.1"
/db_xref="GeneID:856723"
/db_xref="SGD:S000000808"
/translation="MRVRKRQSRRTSTKLKEGIKKKASAHRKKEKKMAKKDVTWRSRS
KKDPGIPSNFPYKAKILEEIEAKKMKDLEERELAKQQRLEARKAAKEQGVDAMDEDMI
EDDENGLAALVESAQQAAAEYEGTPSNDADVRDDELDVIDYNIDFYGEDVEGESELEK
SRKAYDKIFKSVIDASDVILYVLDARDPESTRSRKVEEAVLQSQGKRLILILNKVDLI
PPHVLEQWLNYLKSSFPTIPLRASSGAVNGTSFNRKLSQTTTASALLESLKTYSNNSN
LKRSIVVGVIGYPNVGKSSVINALLARRGGQSKACPVGNEAGVTTSLREIKIDNKLKI
LDSPGICFPSENKKRSKVEHEAELALLNALPAKHIVDPYPAVLMLVKRLAKSDEMTES
FKKLYEIPPIPANDADTFTKHFLIHVARKRGRLGKGGIPNLASAGLSVLNDWRDGKIL
GWVLPNTSAAASQQDKQNLSTINTGTKQAPIAANESTIVSEWSKEFDLDGLFSSLDKA
IDASKDQDTMME"
gene <164527..>166083
/gene="PAC2"
/locus_tag="YER007W"
/db_xref="GeneID:856724"
mRNA <164527..>166083
/gene="PAC2"
/locus_tag="YER007W"
/product="Pac2p"
/transcript_id="NM_001178898.1"
/db_xref="GeneID:856724"
CDS 164527..166083
/gene="PAC2"
/locus_tag="YER007W"
/experiment="EXISTENCE:genetic interaction:GO:0006457
protein folding [PMID:9215891]"
/experiment="EXISTENCE:genetic interaction:GO:0007021
tubulin complex assembly [PMID:9215891]"
/experiment="EXISTENCE:mutant phenotype:GO:0007021 tubulin
complex assembly [PMID:9215891]"
/experiment="EXISTENCE:physical interaction:GO:0043014
alpha-tubulin binding [PMID:9885248]"
/note="Microtubule effector required for tubulin
heterodimer formation; binds alpha-tubulin, required for
normal microtubule function, null mutant exhibits
cold-sensitive microtubules and sensitivity to benomyl"
/codon_start=1
/product="Pac2p"
/protein_id="NP_010922.1"
/db_xref="GeneID:856724"
/db_xref="SGD:S000000809"
/translation="MTYEIGDRLKIGGYFCTIKFIGVIKPWPSVKAYGVEWDDHSRGK
HSGTIDDIHYFDVQIPNSGSFLKESKIKSPGVRRITFYEALSEKYGGSSNNINDLSIG
NKRVEGLGFDELNARNKNYKKLRKIALRDSDVAILFQNQDELNRVIQNCVNVKDLDLS
LNLFTNINSLCEFIEPLKNLESLNISQNKLLSGWDNLKEYDLSHIKTLRLSSCGLSYK
HIGKLLKSFRTLKMLDLSYNNLTSAGIQNFENEIPCTLEELNISGNNLISFPLFPKNL
TLKGLNVSNNQISRAPSIAIYSVESLDITDNKFKERSLIDDLNKTFPSLKNIHLSGNE
FNYNGNYINVEEQATFYEVLARFDRVMVLNGSICDVKTRREAEMFFVSKVMNNELSYD
TNLPRWSSLIKSYEIDMSKLSFNNERETRQSLVLKIKIRAGKKPSSDLDYWVLPSFTV
RYVKSVICRKLNFDILNVKLFHENSEGMINEIKYNFRPISDFNVVNGDIIHVSSPVNN
KSIQKVNSPS"
gene complement(<166237..>166885)
/gene="TMA20"
/locus_tag="YER007C-A"
/db_xref="GeneID:856725"
mRNA complement(join(<166237..166771,166875..>166885))
/gene="TMA20"
/locus_tag="YER007C-A"
/product="translation machinery-associated protein 20"
/transcript_id="NM_001180857.3"
/db_xref="GeneID:856725"
CDS complement(join(166237..166771,166875..166885))
/gene="TMA20"
/locus_tag="YER007C-A"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:23613772]"
/experiment="EXISTENCE:mutant phenotype:GO:0000184
nuclear-transcribed mRNA catabolic process,
nonsense-mediated decay [PMID:38198768]"
/experiment="EXISTENCE:mutant phenotype:GO:0042254
ribosome biogenesis [PMID:16702403]"
/note="Ribosome-associated protein; involved along with
heterodimeric partner Tma22p in recycling of 40S ribosomal
subunits; involved in nonsense-mediated mRNA decay (NMD);
contains a putative RNA binding domain; null mutant
exhibits translation defects; localizes to the cytosol;
has homology to human oncogene MCT-1; protein abundance
increases in response to DNA replication stress"
/codon_start=1
/product="translation machinery-associated protein 20"
/protein_id="NP_010923.3"
/db_xref="GeneID:856725"
/db_xref="SGD:S000002957"
/translation="MFKKFTREDVHSRSKVKSSIQRTLKAKLVKQYPKIEDVIDELIP
KKSQIELIKCEDKIQLYSVDGEVLFFQKFDELIPSLKLVHKFPEAYPTVQVDRGAIKF
VLSGANIMCPGLTSAGADLPPAPGYEKGTIVVINAENKENALAIGELMMGTEEIKSVN
KGHSIELIHHLGDPLWNFSVE"
gene complement(167427..167586)
/gene="SNR14"
/locus_tag="YNCE0011C"
/db_xref="GeneID:9164888"
ncRNA complement(167427..167586)
/ncRNA_class="snRNA"
/gene="SNR14"
/locus_tag="YNCE0011C"
/product="SNR14"
/experiment="EXISTENCE:direct assay:GO:0005681
spliceosomal complex [PMID:2962902]"
/experiment="EXISTENCE:direct assay:GO:0046540 U4/U6 x U5
tri-snRNP complex [PMID:10449419|PMID:10377396]"
/experiment="EXISTENCE:physical interaction:GO:0017069
snRNA binding [PMID:2440583]"
/note="U4 spliceosomal RNA (U4 snRNA); interacts with U6"
/transcript_id="NR_132167.1"
/db_xref="GeneID:9164888"
/db_xref="SGD:S000006499"
gene complement(<167808..>171818)
/gene="SEC3"
/locus_tag="YER008C"
/gene_synonym="PSL1"
/db_xref="GeneID:856726"
mRNA complement(<167808..>171818)
/gene="SEC3"
/locus_tag="YER008C"
/gene_synonym="PSL1"
/product="GTP-Rho binding exocyst subunit SEC3"
/transcript_id="NM_001178899.1"
/db_xref="GeneID:856726"
CDS complement(167808..171818)
/gene="SEC3"
/locus_tag="YER008C"
/gene_synonym="PSL1"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:9491896|PMID:11595741]"
/experiment="EXISTENCE:direct assay:GO:0000145 exocyst
[PMID:8978675]"
/experiment="EXISTENCE:direct assay:GO:0005546
phosphatidylinositol-4,5-bisphosphate binding
[PMID:18195105]"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip
[PMID:17717527|PMID:9491896|PMID:22842922|PMID:11595741]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck
[PMID:22842922|PMID:26928762|PMID:11595741|PMID:9491896]"
/experiment="EXISTENCE:direct assay:GO:0006887 exocytosis
[PMID:15772160]"
/experiment="EXISTENCE:direct assay:GO:0031267 small
GTPase binding [PMID:11283608]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807|PMID:9491896]"
/experiment="EXISTENCE:genetic interaction:GO:0006887
exocytosis [PMID:12913108|PMID:17717527]"
/experiment="EXISTENCE:genetic interaction:GO:0006893
Golgi to plasma membrane transport [PMID:7026045]"
/experiment="EXISTENCE:genetic interaction:GO:0048309
endoplasmic reticulum inheritance [PMID:15583030]"
/experiment="EXISTENCE:genetic interaction:GO:0051601
exocyst localization [PMID:17717527|PMID:18946089]"
/experiment="EXISTENCE:mutant phenotype:GO:0001927 exocyst
assembly [PMID:15583030]"
/experiment="EXISTENCE:mutant phenotype:GO:0006887
exocytosis [PMID:6996832]"
/experiment="EXISTENCE:mutant phenotype:GO:0006893 Golgi
to plasma membrane transport [PMID:7026045]"
/experiment="EXISTENCE:mutant phenotype:GO:0048309
endoplasmic reticulum inheritance
[PMID:12960429|PMID:15583030]"
/experiment="EXISTENCE:physical interaction:GO:0031267
small GTPase binding [PMID:11595741]"
/note="Subunit of the exocyst complex; the exocyst
mediates polarized targeting and tethering of post-Golgi
secretory vesicles to sites of exocytosis prior to
SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that
localizes to exocytic sites in a Rho1p-dependent,
actin-independent manner, targeting and anchoring the
exocyst to the plasma membrane with Exo70p; direct GTP
Rho1p effector; required for ER inheritance; relocalizes
away from bud neck upon DNA replication stress"
/codon_start=1
/product="GTP-Rho binding exocyst subunit SEC3"
/protein_id="NP_010924.1"
/db_xref="GeneID:856726"
/db_xref="SGD:S000000810"
/translation="MRSSKSPFKRKSHSRETSHDENTSFFHKRTISGSSAHHSRNVSQ
GAVPSSAPPVSGGNYSHKRNVSRASNSSQTSNFLAEQYERDRKAIINCCFSRPDHKTG
EPPNNYITHVRIIEDSKFPSSRPPPDSKLENKKKRLLILSAKPNNAKLIQIHKARENS
DGSFQIGRTWQLTELVRVEKDLEISEGFILTMSKKYYWETNSAKERTVFIKSLITLYI
QTFEGHVPELVNWDLSLFYLDERSYQRAVITNRPGSVSPIKSPTSNFTTNTTQSVGSV
PFSAPTERTRRSETESVNPVSTPASVEYHAGMKSLNKAPYSSNSTLNEVNKRYELEQQ
QQQEEAELRRLEEQKRLQLQKENEMKRLEEERRIKQEERKRQMELEHQRQLEEEERKR
QMELEAKKQMELKRQRQFEEEQRLKKERELLEIQRKQREQETAERLKKEEQEALAKKE
EEEKSKRNKVDNESYTQEINGKVDNLLEDLNAVLAEETETTPTMQNGTYVPERSTARA
HDQLKKPLNIAKVESLGGSDLNDSISLSDEIAGLNTSNLSGEDQDEKNDLSFEKGDEV
RYSNNFEGEAPHVYHEVSIIQEEAPAVSQKLILPEENNESEALIESKEEIKTMENIDD
EVLLEILTDINWSIEDDADSMIERIDLRLAETEYLFNQNLLSLQKIGPNIRPYEDKVN
DECHRIIPTLSLFLMEMSNFSNDIENVESQDNGLQVESANKKLLWNTLDELLKTVSLD
EISLNQLLECPIREKNLPWMENQLNLLLKAFQAIGSDGNEVEYNLREISGLKQRLQFY
EKVTKIFLNRIVEEMQKKFSNIRGQDISHDQMIRILTTLLIFSPLILFCKEISQKSYQ
AIVENWNVSIQPVYMELWTKKISQLQGIDTNDEKMNELSLSQLLNEWDTFRKERKTND
INPVFKNSFSLLTECLQTMRQECIVYQNFVEVFFHISSKHNFEEYIKHFNDPDAPPIL
LDTVKVMQSDREAAVIETQLVSRIFQPIVTRLSSYFVELVKAEPTVAPALTFYLENEI
KSLESSNHEFLLSAVTRMYTQIKQVWSDNVEEQVLHFERISNATTNGEILPGILDLPV
GLKNSEDLFQFAKRSMDIKDTDEGYESIELMNSSFRKLSIAATRSITHKEVNSSINPN
LSDTAALNNDYMETISLLVNSNWLTEMLSMLNFNKDGIFDTSLQNVKKVFDVEKESYA
SFLLRDTMPKLTAFVYGVSNIIENTNNVNMTNPSRWAAYSRQNLENILLAYTSHEIET
LVKRLHTHMVNDFGYHQENAINNVLCDKLWSCIQGQTVSLYLKLYTVIDKHYRGTNIR
FTKNDIISAFEEYKNA"
gene <172115..>172492
/gene="NTF2"
/locus_tag="YER009W"
/db_xref="GeneID:856727"
mRNA <172115..>172492
/gene="NTF2"
/locus_tag="YER009W"
/product="Ran GTPase-binding protein NTF2"
/transcript_id="NM_001178900.1"
/db_xref="GeneID:856727"
CDS 172115..172492
/gene="NTF2"
/locus_tag="YER009W"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:8702493]"
/experiment="EXISTENCE:direct assay:GO:0031267 small
GTPase binding [PMID:10889207]"
/experiment="EXISTENCE:mutant phenotype:GO:0006606 protein
import into nucleus [PMID:11024003|PMID:8702493]"
/experiment="EXISTENCE:mutant phenotype:GO:0006913
nucleocytoplasmic transport [PMID:8702493]"
/note="Nuclear envelope protein; interacts with GDP-bound
Gsp1p and with proteins of the nuclear pore to transport
Gsp1p into the nucleus where it is an essential player in
nucleocytoplasmic transport"
/codon_start=1
/product="Ran GTPase-binding protein NTF2"
/protein_id="NP_010925.1"
/db_xref="GeneID:856727"
/db_xref="SGD:S000000811"
/translation="MSLDFNTLAQNFTQFYYNQFDTDRSQLGNLYRNESMLTFETSQL
QGAKDIVEKLVSLPFQKVQHRITTLDAQPASPNGDVLVMITGDLLIDEEQNPQRFSQV
FHLIPDGNSYYVFNDIFRLNYSA"
gene complement(<172634..>173338)
/locus_tag="YER010C"
/db_xref="GeneID:856728"
mRNA complement(<172634..>173338)
/locus_tag="YER010C"
/product="bifunctional 4-hydroxy-4-methyl-2-oxoglutarate
aldolase/oxaloacetate decarboxylase"
/transcript_id="NM_001178901.3"
/db_xref="GeneID:856728"
CDS complement(172634..173338)
/locus_tag="YER010C"
/EC_number="4.1.1.112"
/EC_number="4.1.3.17"
/experiment="EXISTENCE:direct assay:GO:0008948
oxaloacetate decarboxylase activity [PMID:24359411]"
/experiment="EXISTENCE:direct assay:GO:0019619
3,4-dihydroxybenzoate catabolic process [PMID:24359411]"
/experiment="EXISTENCE:direct assay:GO:0047443
4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
[PMID:24359411]"
/note="Bifunctional HMG aldolase/oxaloacetate
decarboxylase; requires divalent metal ions for activity;
competitively inhibited by oxalate; forms a ring-shaped
homotrimer; similar to members of the prokaryotic RraA
family of class II (divalent metal ion dependent) pyruvate
aldolases from the meta cleavage pathways of
protocatechuate and gallate"
/codon_start=1
/product="bifunctional 4-hydroxy-4-methyl-2-oxoglutarate
aldolase/oxaloacetate decarboxylase"
/protein_id="NP_010926.3"
/db_xref="GeneID:856728"
/db_xref="SGD:S000000812"
/translation="MSDLQKLQRFSTCDISDGLLNVYNIPTGGYFPNLTAISPPQNSS
IVGTAYTVLFAPIDDPRPAVNYIDSVPPNSILVLALEPHLQSQFHPFIKITQAMYGGL
MSTRAQYLKSNGTVVFGRIRDVDEHRTLNHPVFAYGVGSCAPKAVVKAVGTNVQLKIL
TSDGVTQTICPGDYIAGDNNGIVRIPVQETDISKLVTYIEKSIEVDLLVSEDIKNGIP
AKQAQNDRRSVLKKYI"
rep_origin 173700..173864
/note="ARS511; Autonomously Replicating Sequence"
/db_xref="SGD:S000077383"
gene <175248..>176012
/gene="TIR1"
/locus_tag="YER011W"
/gene_synonym="SRP1"
/db_xref="GeneID:856729"
mRNA <175248..>176012
/gene="TIR1"
/locus_tag="YER011W"
/gene_synonym="SRP1"
/product="GPI-anchored mannoprotein"
/transcript_id="NM_001178902.1"
/db_xref="GeneID:856729"
CDS 175248..176012
/gene="TIR1"
/locus_tag="YER011W"
/gene_synonym="SRP1"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005199 structural
constituent of cell wall [PMID:9363789]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:15781460|PMID:8733242]"
/experiment="EXISTENCE:direct assay:GO:0031505 fungal-type
cell wall organization [PMID:9363789]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="Cell wall mannoprotein; expression is downregulated
at acidic pH and induced by cold shock and anaerobiosis;
abundance is increased in cells cultured without shaking;
member of the Srp1p/Tip1p family of serine-alanine-rich
proteins"
/codon_start=1
/product="GPI-anchored mannoprotein"
/protein_id="NP_010927.1"
/db_xref="GeneID:856729"
/db_xref="SGD:S000000813"
/translation="MAYTKIALFAAIAALASAQTQDQINELNVILNDVKSHLQEYISL
ASDSSSGFSLSSMPAGVLDIGMALASATDDSYTTLYSEVDFAGVSKMLTMVPWYSSRL
EPALKSLNGDASSSAAPSSSAAPTSSAAPSSSAAPTSSAASSSSEAKSSSAAPSSSEA
KSSSAAPSSSEAKSSSAAPSSSEAKSSSAAPSSTEAKITSAAPSSTGAKTSAISQITD
GQIQATKAVSEQTENGAAKAFVGMGAGVVAAAAMLL"
repeat_region 176705..176856
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006912"
gene 177099..177170
/locus_tag="YNCE0012W"
/db_xref="GeneID:856730"
tRNA 177099..177170
/locus_tag="YNCE0012W"
/product="tRNA-Glu"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE
analysis; thiolation of uridine at wobble position (34)
requires Ncs6p; target of K. lactis zymocin"
/db_xref="GeneID:856730"
/db_xref="SGD:S000006550"
gene <177835..>178431
/gene="PRE1"
/locus_tag="YER012W"
/db_xref="GeneID:856731"
mRNA <177835..>178431
/gene="PRE1"
/locus_tag="YER012W"
/product="proteasome core particle subunit beta 4"
/transcript_id="NM_001178903.1"
/db_xref="GeneID:856731"
CDS 177835..178431
/gene="PRE1"
/locus_tag="YER012W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10419517]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:15973433]"
/experiment="EXISTENCE:direct assay:GO:0010499 proteasomal
ubiquitin-independent protein catabolic process
[PMID:19162040]"
/experiment="EXISTENCE:direct assay:GO:0019774 proteasome
core complex, beta-subunit complex [PMID:9087403]"
/experiment="EXISTENCE:direct assay:GO:0043161
proteasome-mediated ubiquitin-dependent protein catabolic
process [PMID:11545745|PMID:19029916]"
/experiment="EXISTENCE:mutant phenotype:GO:0061133
endopeptidase activator activity [PMID:8808631]"
/note="Beta 4 subunit of the 20S proteasome; localizes to
the nucleus throughout the cell cycle"
/codon_start=1
/product="proteasome core particle subunit beta 4"
/protein_id="NP_010928.1"
/db_xref="GeneID:856731"
/db_xref="SGD:S000000814"
/translation="MDIILGIRVQDSVILASSKAVTRGISVLKDSDDKTRQLSPHTLM
SFAGEAGDTVQFAEYIQANIQLYSIREDYELSPQAVSSFVRQELAKSIRSRRPYQVNV
LIGGYDKKKNKPELYQIDYLGTKVELPYGAHGYSGFYTFSLLDHHYRPDMTTEEGLDL
LKLCVQELEKRMPMDFKGVIVKIVDKDGIRQVDDFQAQ"
gene <178841..>182278
/gene="PRP22"
/locus_tag="YER013W"
/db_xref="GeneID:856732"
mRNA <178841..>182278
/gene="PRP22"
/locus_tag="YER013W"
/product="DEAH-box ATP-dependent RNA helicase PRP22"
/transcript_id="NM_001178904.3"
/db_xref="GeneID:856732"
CDS 178841..182278
/gene="PRP22"
/locus_tag="YER013W"
/EC_number="3.6.4.13"
/experiment="EXISTENCE:direct assay:GO:0000350 generation
of catalytic spliceosome for second transesterification
step [PMID:12212850]"
/experiment="EXISTENCE:direct assay:GO:0000386 second
spliceosomal transesterification activity [PMID:12212850]"
/experiment="EXISTENCE:direct assay:GO:0003724 RNA
helicase activity [PMID:9582286]"
/experiment="EXISTENCE:mutant phenotype:GO:0000350
generation of catalytic spliceosome for second
transesterification step [PMID:22408182]"
/experiment="EXISTENCE:mutant phenotype:GO:0000386 second
spliceosomal transesterification activity [PMID:9524130]"
/experiment="EXISTENCE:mutant phenotype:GO:0000390
spliceosomal complex disassembly [PMID:9524130]"
/experiment="EXISTENCE:mutant phenotype:GO:0071007 U2-type
catalytic step 2 spliceosome [PMID:9524130]"
/experiment="EXISTENCE:mutant phenotype:GO:0071021 U2-type
post-spliceosomal complex [PMID:12212850]"
/note="DEAH-box RNA-dependent ATPase/ATP-dependent RNA
helicase; associates with lariat intermediates before the
second catalytic step of splicing; mediates ATP-dependent
mRNA release from the spliceosome and unwinds RNA
duplexes; required for proofreading the exon ligation
reaction"
/codon_start=1
/product="DEAH-box ATP-dependent RNA helicase PRP22"
/protein_id="NP_010929.3"
/db_xref="GeneID:856732"
/db_xref="SGD:S000000815"
/translation="MSDISKLIGAIVGSDDPVIIEFVLNIINKSGNLQEFIRNIQKLD
AGISYEDSIKMYNAFLGKQEEEKVRNKVKSSPLSQKINQVLKDDVNLDDPVVTEFVLS
ILNKSKSITEFQEQLNLMQSGLDNETIFKIYQIASPPVMKEEVSVLPSTKIPAKIEAK
IEEEVQKIESLDPSPVLHKVYEGKVRNITTFGCFVQIFGTRMKNCDGLVHISEMSDQR
TLDPHDVVRQGQHIFVEVIKIQNNGKISLSMKNIDQHSGEIRKRNTESVEDRGRSNDA
HTSRNMKNKIKRRALTSPERWEIRQLIASGAASIDDYPELKDEIPINTSYLTAKRDDG
SIVNGNTEKVDSKLEEQQRDETDEIDVELNTDDGPKFLKDQQVKGAKKYEMPKITKVP
RGFMNRSAINGSNAIRDHREEKLRKKREIEQQIRKQQSFDDPTKNKKDSRNEIQMLKN
QLIVTEWEKNRMNESISYGKRTSLPISAQRQTLPVYAMRSELIQAVRDNQFLVIVGET
GSGKTTQITQYLDEEGFSNYGMIGCTQPRRVAAVSVAKRVAEEVGCKVGHDVGYTIRF
EDVTGPDTRIKYMTDGMLQREALLDPEMSKYSVIMLDEAHERTVATDVLFALLKKAAI
KRPELKVIVTSATLNSAKFSEYFLNCPIINIPGKTFPVEVLYSQTPQMDYIEAALDCV
IDIHINEGPGDILVFLTGQEEIDSCCEILYDRVKTLGDSIGELLILPVYSALPSEIQS
KIFEPTPKGSRKVVFATNIAETSITIDGIYYVVDPGFAKINIYNARAGIEQLIVSPIS
QAQANQRKGRAGRTGPGKCYRLYTESAFYNEMLENTVPEIQRQNLSHTILMLKAMGIN
DLLKFDFMDPPPKNLMLNALTELYHLQSLDDEGKLTNLGKEMSLFPMDPTLSRSLLSS
VDNQCSDEIVTIISMLSVQNVFYRPKDRQLEADSKKAKFHHPYGDHLTLLNVYTRWQQ
ANYSEQYCKTNFLHFRHLKRARDVKSQISMIFKKIGLKLISCHSDPDLIRKTFVSGFF
MNAAKRDSQVGYKTINGGTEVGIHPSSSLYGKEYEYVMYHSIVLTSREYMSQVTSIEP
QWLLEVAPHFYKAGDAESQSRKKAKIIPLHNKFAKDQNSWRLSSIRQSRERALGIKR"
gene <182600..>184219
/gene="HEM14"
/locus_tag="YER014W"
/db_xref="GeneID:856733"
mRNA <182600..>184219
/gene="HEM14"
/locus_tag="YER014W"
/product="oxygen-dependent protoporphyrinogen oxidase"
/transcript_id="NM_001178905.1"
/db_xref="GeneID:856733"
CDS 182600..184219
/gene="HEM14"
/locus_tag="YER014W"
/EC_number="1.3.3.4"
/experiment="EXISTENCE:direct assay:GO:0004729
oxygen-dependent protoporphyrinogen oxidase activity
[PMID:7798202]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:24769239|PMID:16823961|PMID:14576278|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:7798202]"
/experiment="EXISTENCE:mutant phenotype:GO:0004729
oxygen-dependent protoporphyrinogen oxidase activity
[PMID:7052077]"
/experiment="EXISTENCE:mutant phenotype:GO:0006783 heme
biosynthetic process [PMID:7035824]"
/note="Protoporphyrinogen oxidase; a mitochondrial enzyme
that catalyzes the seventh step in the heme biosynthetic
pathway, converting protoporphyrinogen IX to
protoporphyrin IX; inhibited by diphenyl ether-type
herbicides"
/codon_start=1
/product="oxygen-dependent protoporphyrinogen oxidase"
/protein_id="NP_010930.1"
/db_xref="GeneID:856733"
/db_xref="SGD:S000000816"
/translation="MLLPLTKLKPRAKVAVVGGGVSGLCFTYFLSKLRPDVEITLFES
QNRTGGWIYSCNTRDMSGNPIMLEKGPRTLRGVSDGTVLIMDTLKDLGKEAVIQSIDK
GCIADKKFLLDPSDKLVQVPNSISTTVKFLLNPLGKGLITGMMGEWFRKKSPHPGQDE
SVESICDRRFGNNYISNNMISALLRGIYGDDVSLLSAKRTFKKIYYNELKHGSNTQAM
IDNMRGKSRSKKTENLHQSLTGCLNDYSNAFGKDRSKLLDLSNTLKKYPMLGLAGGLE
TFPKIVRNALNEFKNVKIVTGNPVTQIMKRPANETTIGLKAKSGDQYETFDHLRLTIT
PPKIAKLLPKDQNSLSKLLDEIQSNTIILVNYYLPNKDVIDADLQGFGYLVPKSNKNP
GKLLGVIFDSVIERNFKPLFDKLSTNPNALNKYTKVTAMIGGCMLNEHGVPVVPSREV
TINAVKDALNNHLGISNKDLEAGQWEFTIADRCLPRFHVGYDAWQERAERKLQESYGQ
TVSVGGMGFSRSPGVPDVIVDGFNDALQLSK"
gene complement(<183731..>184700)
/gene="BUD25"
/locus_tag="YER014C-A"
/db_xref="GeneID:856735"
mRNA complement(join(<183731..184169,184678..>184700))
/gene="BUD25"
/locus_tag="YER014C-A"
/product="Bud25p"
/transcript_id="NM_001184456.1"
/db_xref="GeneID:856735"
CDS complement(join(183731..184169,184678..184700))
/gene="BUD25"
/locus_tag="YER014C-A"
/experiment="EXISTENCE:mutant phenotype:GO:0000282
cellular bud site selection [PMID:11452010]"
/note="Protein involved in bud-site selection; diploid
mutants display a random budding pattern instead of the
wild-type bipolar pattern"
/codon_start=1
/product="Bud25p"
/protein_id="NP_076437.1"
/db_xref="GeneID:856735"
/db_xref="SGD:S000007590"
/translation="MFCTWDSWDTGTSRKSHSPHRNCLAVRFLQLPFSSFLPCVITYM
KSWQTSIGDSEFPLTSFQILVTDAEVVVQRIFDCINGYLPGWHYRNTVFIEHTTSYHR
SHFCIFVEGVWVCGEFVKKWFEIPFDNRIENYTKQFPWILIGFGNQVSKAL"
gene <184541..>186775
/gene="FAA2"
/locus_tag="YER015W"
/gene_synonym="FAM1"
/db_xref="GeneID:856734"
mRNA <184541..>186775
/gene="FAA2"
/locus_tag="YER015W"
/gene_synonym="FAM1"
/product="medium-chain fatty acid-CoA ligase FAA2"
/transcript_id="NM_001178906.3"
/db_xref="GeneID:856734"
CDS 184541..186775
/gene="FAA2"
/locus_tag="YER015W"
/gene_synonym="FAM1"
/EC_number="6.2.1.3"
/experiment="EXISTENCE:direct assay:GO:0004467 long-chain
fatty acid-CoA ligase activity [PMID:8206942]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:8670886]"
/experiment="EXISTENCE:direct assay:GO:0031956
medium-chain fatty acid-CoA ligase activity
[PMID:8206942]"
/experiment="EXISTENCE:genetic interaction:GO:0001676
long-chain fatty acid metabolic process [PMID:7962057]"
/experiment="EXISTENCE:genetic interaction:GO:0031957 very
long-chain fatty acid-CoA ligase activity [PMID:9988704]"
/experiment="EXISTENCE:mutant phenotype:GO:0001676
long-chain fatty acid metabolic process [PMID:7962057]"
/note="Medium chain fatty acyl-CoA synthetase; activates
imported fatty acids; accepts a wide range of fatty acid
chain lengths with a preference for medium chains,
C9:0-C13:0; localized to the peroxisome; comparative
analysis suggests that a mitochondrially targeted form may
result from translation starting at a non-canonical codon
upstream of the annotated start codon"
/codon_start=1
/product="medium-chain fatty acid-CoA ligase FAA2"
/protein_id="NP_010931.3"
/db_xref="GeneID:856734"
/db_xref="SGD:S000000817"
/translation="MAAPDYALTDLIESDPRFESLKTRLAGYTKGSDEYIEELYSQLP
LTSYPRYKTFLKKQAVAISNPDNEAGFSSIYRSSLSSENLVSCVDKNLRTAYDHFMFS
ARRWPQRDCLGSRPIDKATGTWEETFRFESYSTVSKRCHNIGSGILSLVNTKRKRPLE
ANDFVVAILSHNNPEWILTDLACQAYSLTNTALYETLGPNTSEYILNLTEAPILIFAK
SNMYHVLKMVPDMKFVNTLVCMDELTHDELRMLNESLLPVKCNSLNEKITFFSLEQVE
QVGCFNKIPAIPPTPDSLYTISFTSGTTGLPKGVEMSHRNIASGIAFAFSTFRIPPDK
RNQQLYDMCFLPLAHIFERMVIAYDLAIGFGIGFLHKPDPTVLVEDLKILKPYAVALV
PRILTRFEAGIKNALDKSTVQRNVANTILDSKSARFTARGGPDKSIMNFLVYHRVLID
KIRDSLGLSNNSFIITGSAPISKDTLLFLRSALDIGIRQGYGLTETFAGVCLSEPFEK
DVGSCGAIGISAECRLKSVPEMGYHADKDLKGELQIRGPQVFERYFKNPNETSKAVDQ
DGWFSTGDVAFIDGKGRISVIDRVKNFFKLAHGEYIAPEKIENIYLSSCPYITQIFVF
GDPLKTFLVGIVGVDVDAAQPILAAKHPEVKTWTKEVLVENLNRNKKLRKEFLNKINK
CTDGLQGFEKLHNIKVGLEPLTLEDDVVTPTFKIKRAKASKFFKDTLDQLYAEGSLVK
TEKL"
gene <188277..>189311
/gene="BIM1"
/locus_tag="YER016W"
/gene_synonym="YEB1"
/db_xref="GeneID:856736"
mRNA <188277..>189311
/gene="BIM1"
/locus_tag="YER016W"
/gene_synonym="YEB1"
/product="microtubule-binding protein BIM1"
/transcript_id="NM_001178907.1"
/db_xref="GeneID:856736"
CDS 188277..189311
/gene="BIM1"
/locus_tag="YER016W"
/gene_synonym="YEB1"
/experiment="EXISTENCE:direct assay:GO:0000922 spindle
pole [PMID:10352017]"
/experiment="EXISTENCE:direct assay:GO:0005881 cytoplasmic
microtubule [PMID:10352017]"
/experiment="EXISTENCE:direct assay:GO:0031116 positive
regulation of microtubule polymerization [PMID:20392838]"
/experiment="EXISTENCE:direct assay:GO:0035371 microtubule
plus-end [PMID:20392838]"
/experiment="EXISTENCE:direct assay:GO:0051010 microtubule
plus-end binding [PMID:20392838]"
/experiment="EXISTENCE:direct assay:GO:0051233 spindle
midzone [PMID:10352017]"
/experiment="EXISTENCE:direct assay:GO:0072686 mitotic
spindle [PMID:10352017]"
/experiment="EXISTENCE:genetic interaction:GO:0007064
mitotic sister chromatid cohesion [PMID:14742714]"
/experiment="EXISTENCE:genetic interaction:GO:0030543
2-micrometer plasmid partitioning [PMID:28004422]"
/experiment="EXISTENCE:genetic interaction:GO:0031578
mitotic spindle orientation checkpoint signaling
[PMID:11285282]"
/experiment="EXISTENCE:mutant phenotype:GO:0007019
microtubule depolymerization [PMID:10352017]"
/experiment="EXISTENCE:mutant phenotype:GO:0007020
microtubule nucleation [PMID:33752231]"
/experiment="EXISTENCE:mutant phenotype:GO:0007026
negative regulation of microtubule depolymerization
[PMID:10352017]"
/experiment="EXISTENCE:mutant phenotype:GO:0007064 mitotic
sister chromatid cohesion [PMID:14742714]"
/experiment="EXISTENCE:mutant phenotype:GO:0030473 nuclear
migration along microtubule [PMID:10352017]"
/experiment="EXISTENCE:mutant phenotype:GO:0030543
2-micrometer plasmid partitioning [PMID:28004422]"
/experiment="EXISTENCE:mutant phenotype:GO:1904825 protein
localization to microtubule plus-end [PMID:29576319]"
/note="Microtubule plus end-tracking protein; forms a
complex with Kar9p that makes up the cortical microtubule
capture site and delays the exit from mitosis when the
spindle is oriented abnormally; homolog of human end
binding protein 1 (EB1)"
/codon_start=1
/product="microtubule-binding protein BIM1"
/protein_id="NP_010932.1"
/db_xref="GeneID:856736"
/db_xref="SGD:S000000818"
/translation="MSAGIGESRTELLTWLNGLLNLNYKKIEECGTGAAYCQIMDSIY
GDLPMNRVKFNATAEYEFQTNYKILQSCFSRHGIEKTVYVDKLIRCKFQDNLEFLQWL
KKHWIRHKDESVYDPDARRKYRPIITNNSATKPRTVSNPTTAKRSSSTGTGSAMSGGL
ATRHSSLGINGSRKTSVTQGQLVAIQAELTKSQETIGSLNEEIEQYKGTVSTLEIERE
FYFNKLRDIEILVHTTQDLINEGVYKFNDETITGHGNGNGGALLRFVKKVESILYATA
EGFEMNDGEDELNDKNLGEHGTVPNQGGYANSNGEVNGNEGSNHDVIMQNDEGEVGVS
NNLIIDEETF"
gene complement(<189503..>191788)
/gene="AFG3"
/locus_tag="YER017C"
/gene_synonym="YTA10"
/db_xref="GeneID:856737"
mRNA complement(<189503..>191788)
/gene="AFG3"
/locus_tag="YER017C"
/gene_synonym="YTA10"
/product="AAA family ATPase AFG3"
/transcript_id="NM_001178908.1"
/db_xref="GeneID:856737"
CDS complement(189503..191788)
/gene="AFG3"
/locus_tag="YER017C"
/gene_synonym="YTA10"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:7926051]"
/experiment="EXISTENCE:direct assay:GO:0005745 m-AAA
complex [PMID:8681382]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:19748354]"
/experiment="EXISTENCE:direct assay:GO:0097002
mitochondrial inner boundary membrane [PMID:19019989]"
/experiment="EXISTENCE:mutant phenotype:GO:0006465 signal
peptide processing [PMID:12417197]"
/experiment="EXISTENCE:mutant phenotype:GO:0008237
metallopeptidase activity [PMID:8681382]"
/experiment="EXISTENCE:mutant phenotype:GO:0016887 ATP
hydrolysis activity [PMID:19748354]"
/experiment="EXISTENCE:mutant phenotype:GO:0030150 protein
import into mitochondrial matrix [PMID:27398316]"
/experiment="EXISTENCE:mutant phenotype:GO:0065003
protein-containing complex assembly [PMID:8681382]"
/note="Mitochondrial inner membrane m-AAA protease
component; mediates degradation of misfolded or
unassembled proteins; also required for correct assembly
of mitochondrial enzyme complexes; involved in cytoplasmic
mRNA translation and aging; expression of human homolog
AFG3L2 can complement yeast yta12 afg3 double mutant"
/codon_start=1
/product="AAA family ATPase AFG3"
/protein_id="NP_010933.1"
/db_xref="GeneID:856737"
/db_xref="SGD:S000000819"
/translation="MMMWQRYARGAPRSLTSLSFGKASRISTVKPVLRSRMPVHQRLQ
TLSGLATRNTIHRSTQIRSFHISWTRLNENRPNKEGEGKNNGNKDNNSNKEDGKDKRN
EFGSLSEYFRSKEFANTMFLTIGFTIIFTLLTPSSNNSGDDSNRVLTFQDFKTKYLEK
GLVSKIYVVNKFLVEAELVNTKQVVSFTIGSVDIFEEQMDQIQDLLNIPPRDRIPIKY
IERSSPFTFLFPFLPTIILLGGLYFITRKINSSPPNANGGGGGGLGGMFNVGKSRAKL
FNKETDIKISFKNVAGCDEAKQEIMEFVHFLKNPGKYTKLGAKIPRGAILSGPPGTGK
TLLAKATAGEANVPFLSVSGSEFVEMFVGVGASRVRDLFTQARSMAPSIIFIDEIDAI
GKERGKGGALGGANDEREATLNQLLVEMDGFTTSDQVVVLAGTNRPDVLDNALMRPGR
FDRHIQIDSPDVNGRQQIYLVHLKRLNLDPLLTDDMNNLSGKLATLTPGFTGADIANA
CNEAALIAARHNDPYITIHHFEQAIERVIAGLEKKTRVLSKEEKRSVAYHEAGHAVCG
WFLKYADPLLKVSIIPRGQGALGYAQYLPPDQYLISEEQFRHRMIMALGGRVSEELHF
PSVTSGAHDDFKKVTQMANAMVTSLGMSPKIGYLSFDQNDGNFKVNKPFSNKTARTID
LEVKSIVDDAHRACTELLTKNLDKVDLVAKELLRKEAITREDMIRLLGPRPFKERNEA
FEKYLDPKSNTEPPEAPAATN"
gene complement(<191959..>192624)
/gene="SPC25"
/locus_tag="YER018C"
/db_xref="GeneID:856738"
mRNA complement(<191959..>192624)
/gene="SPC25"
/locus_tag="YER018C"
/product="kinetochore-associated Ndc80 complex subunit
SPC25"
/transcript_id="NM_001178909.3"
/db_xref="GeneID:856738"
CDS complement(191959..192624)
/gene="SPC25"
/locus_tag="YER018C"
/experiment="EXISTENCE:direct assay:GO:0000776 kinetochore
[PMID:11511347]"
/experiment="EXISTENCE:direct assay:GO:0031262 Ndc80
complex [PMID:11266451|PMID:11179222]"
/experiment="EXISTENCE:mutant phenotype:GO:0000776
kinetochore [PMID:11179222]"
/experiment="EXISTENCE:mutant phenotype:GO:0007059
chromosome segregation [PMID:11266451]"
/experiment="EXISTENCE:mutant phenotype:GO:0098653
centromere clustering [PMID:11179222]"
/experiment="EXISTENCE:physical interaction:GO:0031262
Ndc80 complex [PMID:11179222]"
/note="Component of the kinetochore-associated Ndc80
complex; involved in chromosome segregation, spindle
checkpoint activity, and kinetochore clustering;
evolutionarily conserved; other members include Ndc80p,
Nuf2p, Spc24p, and Spc25p"
/codon_start=1
/product="kinetochore-associated Ndc80 complex subunit
SPC25"
/protein_id="NP_010934.3"
/db_xref="GeneID:856738"
/db_xref="SGD:S000000820"
/translation="MASIDAFSDLERRMDGFQKDVAQVLARQQNHARQQLQQFQAEMR
QLHNQHQHLIDELQRLATQRTALQQQIHAAQQATNTTREQWRSYHERESELSRRQSTL
AAQSRELDSLLQQRGKECVQLRARWAAQSGNDAAEVALYERLLQLRVLPGASDVHDVR
FVFGDDSRCWIEVAMHGDHVIGNSHPALDPKSRATLEHVLTVQGDLAAFLVVARDMLL
ASL"
gene <192797..>194230
/gene="ISC1"
/locus_tag="YER019W"
/db_xref="GeneID:856739"
mRNA <192797..>194230
/gene="ISC1"
/locus_tag="YER019W"
/product="inositol phosphosphingolipid phospholipase"
/transcript_id="NM_001178910.1"
/db_xref="GeneID:856739"
CDS 192797..194230
/gene="ISC1"
/locus_tag="YER019W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14699160]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:17880915]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762|PMID:14699160]"
/experiment="EXISTENCE:direct assay:GO:0030149
sphingolipid catabolic process [PMID:11006294]"
/experiment="EXISTENCE:direct assay:GO:0052712 inositol
phosphosphingolipid phospholipase activity
[PMID:11006294]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0046513
ceramide biosynthetic process
[PMID:23620586|PMID:17880915]"
/experiment="EXISTENCE:mutant phenotype:GO:0072711
cellular response to hydroxyurea [PMID:23620586]"
/experiment="EXISTENCE:mutant phenotype:GO:0090266
regulation of mitotic cell cycle spindle assembly
checkpoint [PMID:32205408]"
/note="Inositol phosphosphingolipid phospholipase C;
hydrolyzes complex sphingolipids to produce ceramide;
regulates the spindle assembly checkpoint upstream of
PP2A-Cdc55; activates genes required for non-fermentable
carbon source metabolism during the diauxic shift;
mediates Na+ and Li+ halotolerance; activated by
phosphatidylserine, cardiolipin and phosphatidylglycerol;
mitochondrial outer membrane protein; ortholog of
mammalian neutral sphingomyelinase type 2"
/codon_start=1
/product="inositol phosphosphingolipid phospholipase"
/protein_id="NP_010935.1"
/db_xref="GeneID:856739"
/db_xref="SGD:S000000821"
/translation="MYNRKDRDVHERKEDGQSEFEALNGTNAIMSDNSKAYSIKFLTF
NTWGLKYVSKHRKERLRAIADKLAGHSMLTPISDELLPNGGDSNENEDYDVIALQEIW
CVEDWKYLASACASKYPYQRLFHSGILTGPGLAILSKVPIESTFLYRFPINGRPSAVF
RGDWYVGKSIAITVLNTGTRPIAIMNSHMHAPYAKQGDAAYLCHRSCQAWDFSRLIKL
YRQAGYAVIVVGDLNSRPGSLPHKFLTQEAGLVDSWEQLHGKQDLAVIARLSPLQQLL
KGCTTCDSLLNTWRAQRQPDEACRLDYALIDPDFLQTVDAGVRFTERIPHLDCSVSDH
FAYSCTLNIVPQGTESRPSTSVKRAKTHDRELILQRYSNYETMIECIHTYLKTAQRQK
FFRGLHFWASILLLIASLVVTTFTANKAGWSSIFWVLFAIAVSISGTIDGAISFLFGR
SEIRALIEVEQEVLDAEHHLQTFLSEK"
gene complement(<194273..>194539)
/gene="SBH2"
/locus_tag="YER019C-A"
/gene_synonym="SEB2"
/db_xref="GeneID:856740"
mRNA complement(<194273..>194539)
/gene="SBH2"
/locus_tag="YER019C-A"
/gene_synonym="SEB2"
/product="Arf family guanine nucleotide exchange factor
SBH2"
/transcript_id="NM_001180031.1"
/db_xref="GeneID:856740"
CDS complement(194273..194539)
/gene="SBH2"
/locus_tag="YER019C-A"
/gene_synonym="SEB2"
/experiment="EXISTENCE:direct assay:GO:0005085
guanyl-nucleotide exchange factor activity
[PMID:12750387]"
/experiment="EXISTENCE:direct assay:GO:0005637 nuclear
inner membrane [PMID:27831485]"
/experiment="EXISTENCE:direct assay:GO:0071261 Ssh1
translocon complex [PMID:8612571]"
/experiment="EXISTENCE:genetic interaction:GO:0006614
SRP-dependent cotranslational protein targeting to
membrane [PMID:18347066]"
/note="Ssh1p-Sss1p-Sbh2p complex component; involved in
protein translocation into the endoplasmic reticulum; SBH2
has a paralog, SBH1, that arose from the whole genome
duplication"
/codon_start=1
/product="Arf family guanine nucleotide exchange factor
SBH2"
/protein_id="NP_010936.1"
/db_xref="GeneID:856740"
/db_xref="SGD:S000002127"
/translation="MAASVPPGGQRILQKRRQAQSIKEKQAKQTPTSTRQAGYGGSSS
SILKLYTDEANGFRVDSLVVLFLSVGFIFSVIALHLLTKFTHII"
gene <195168..>196517
/gene="GPA2"
/locus_tag="YER020W"
/gene_synonym="SSP101"
/db_xref="GeneID:856741"
mRNA <195168..>196517
/gene="GPA2"
/locus_tag="YER020W"
/gene_synonym="SSP101"
/product="guanine nucleotide-binding protein subunit
alpha"
/transcript_id="NM_001178911.3"
/db_xref="GeneID:856741"
CDS 195168..196517
/gene="GPA2"
/locus_tag="YER020W"
/gene_synonym="SSP101"
/experiment="EXISTENCE:direct assay:GO:0003924 GTPase
activity [PMID:16924114]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:31690628]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:31690628|PMID:16622836|PMID:16030250]"
/experiment="EXISTENCE:genetic interaction:GO:0010255
glucose mediated signaling pathway
[PMID:2830616|PMID:10361302]"
/experiment="EXISTENCE:mutant phenotype:GO:0001403
invasive growth in response to glucose limitation
[PMID:12150916]"
/experiment="EXISTENCE:mutant phenotype:GO:0003924 GTPase
activity [PMID:9384580]"
/experiment="EXISTENCE:mutant phenotype:GO:0007124
pseudohyphal growth
[PMID:9384580|PMID:10655215|PMID:10454558]"
/experiment="EXISTENCE:mutant phenotype:GO:0007165 signal
transduction [PMID:10454558]"
/experiment="EXISTENCE:mutant phenotype:GO:0007189
adenylate cyclase-activating G protein-coupled receptor
signaling pathway [PMID:9628870]"
/experiment="EXISTENCE:mutant phenotype:GO:0008361
regulation of cell size [PMID:12089449]"
/experiment="EXISTENCE:mutant phenotype:GO:0009757 hexose
mediated signaling [PMID:10655215]"
/experiment="EXISTENCE:mutant phenotype:GO:0010255 glucose
mediated signaling pathway [PMID:9628870|PMID:2830616]"
/experiment="EXISTENCE:mutant phenotype:GO:0030437
ascospore formation [PMID:10454558]"
/experiment="EXISTENCE:physical interaction:GO:0007189
adenylate cyclase-activating G protein-coupled receptor
signaling pathway [PMID:16924114]"
/note="Nucleotide binding alpha subunit of the
heterotrimeric G protein; interacts with the receptor
Gpr1p, has signaling role in response to nutrients;
required for the recruitment of Ras-GTP at the plasma
membrane and in the nucleus"
/codon_start=1
/product="guanine nucleotide-binding protein subunit
alpha"
/protein_id="NP_010937.3"
/db_xref="GeneID:856741"
/db_xref="SGD:S000000822"
/translation="MGLCASSEKNGSTPDTQTASAGSDNVGKAKVPPKQEPQKTVRTV
NTANQQEKQQQRQQQPSPHNVKDRKEQNGSINNAISPTATANTSGSQQINIDSALRDR
SSNVAAQPSLSDASSGSNDKELKVLLLGAGESGKSTVLQQLKILHQNGFSEQEIKEYI
PLIYQNLLEIGRNLIQARTRFNVNLEPECELTQQDLSRTMSYEMPNNYTGQFPEDIAG
VISTLWALPSTQDLVNGPNASKFYLMDSTPYFMENFTRITSPNYRPTQQDILRSRQMT
SGIFDTVIDMGSDIKMHIYDVGGQRSERKKWIHCFDNVTLVIFCVSLSEYDQTLMEDK
NQNRFQESLVLFDNIVNSRWFARTSVVLFLNKIDLFAEKLSKVPMENYFPDYTGGSDI
NKAAKYILWRFVQLNRANLSIYPHVTQATDTSNIRLVFAAIKETILENTLKDSGVLQ"
gene <196948..>198519
/gene="RPN3"
/locus_tag="YER021W"
/gene_synonym="SUN2"
/db_xref="GeneID:856742"
mRNA <196948..>198519
/gene="RPN3"
/locus_tag="YER021W"
/gene_synonym="SUN2"
/product="proteasome regulatory particle lid subunit RPN3"
/transcript_id="NM_001178912.1"
/db_xref="GeneID:856742"
CDS 196948..198519
/gene="RPN3"
/locus_tag="YER021W"
/gene_synonym="SUN2"
/experiment="EXISTENCE:direct assay:GO:0008541 proteasome
regulatory particle, lid subcomplex [PMID:9741626]"
/note="Essential non-ATPase regulatory subunit of the 26S
proteasome lid; similar to the p58 subunit of the human
26S proteasome; temperature-sensitive alleles cause
metaphase arrest, suggesting a role for the proteasome in
cell cycle control"
/codon_start=1
/product="proteasome regulatory particle lid subunit RPN3"
/protein_id="NP_010938.1"
/db_xref="GeneID:856742"
/db_xref="SGD:S000000823"
/translation="MASTAVMMDVDSSGVNDLHHSEKKYAEEDQVQELLKVLNEISKT
TLTLDPRYIWRSLKDLSSLRNQELLNAETLCFTVNVLYPDSSSFKKNLLKFITSNHKS
SVPGSAELRNSYPASFYSVNTEKKTIEVTAEINCFMHLLVQLFLWDSKELEQLVEFNR
KVVIPNLLCYYNLRSLNLINAKLWFYIYLSHETLARSSEEINSDNQNIILRSTMMKFL
KIASLKHDNETKAMLINLILRDFLNNGEVDSASDFISKLEYPHTDVSSSLEARYFFYL
SKINAIQLDYSTANEYIIAAIRKAPHNSKSLGFLQQSNKLHCCIQLLMGDIPELSFFH
QSNMQKSLLPYYHLTKAVKLGDLKKFTSTITKYKQLLLKDDTYQLCVRLRSNVIKTGI
RIISLTYKKISLRDICLKLNLDSEQTVEYMVSRAIRDGVIEAKINHEDGFIETTELLN
IYDSEDPQQVFDERIKFANQLHDEYLVSMRYPEDKKTQQNEKSENGENDDDTLDGDLM
DDMSDISDLDDLGFL"
gene <198812..>200875
/gene="SRB4"
/locus_tag="YER022W"
/gene_synonym="MED17"
/db_xref="GeneID:856743"
mRNA <198812..>200875
/gene="SRB4"
/locus_tag="YER022W"
/gene_synonym="MED17"
/product="Srb4p"
/transcript_id="NM_001178913.1"
/db_xref="GeneID:856743"
CDS 198812..200875
/gene="SRB4"
/locus_tag="YER022W"
/gene_synonym="MED17"
/experiment="EXISTENCE:direct assay:GO:0000979 RNA
polymerase II core promoter sequence-specific DNA binding
[PMID:23447536]"
/experiment="EXISTENCE:direct assay:GO:0034605 cellular
response to heat [PMID:23447536]"
/experiment="EXISTENCE:direct assay:GO:0045944 positive
regulation of transcription by RNA polymerase II
[PMID:8187178]"
/experiment="EXISTENCE:direct assay:GO:0070847 core
mediator complex [PMID:9891034]"
/experiment="EXISTENCE:direct assay:GO:0140297 DNA-binding
transcription factor binding [PMID:9660972]"
/experiment="EXISTENCE:mutant phenotype:GO:0001139 RNA
polymerase II complex recruiting activity [PMID:19805365]"
/experiment="EXISTENCE:mutant phenotype:GO:0006367
transcription initiation at RNA polymerase II promoter
[PMID:16263706]"
/experiment="EXISTENCE:mutant phenotype:GO:0051123 RNA
polymerase II preinitiation complex assembly
[PMID:33789343]"
/experiment="EXISTENCE:physical interaction:GO:0001139 RNA
polymerase II complex recruiting activity [PMID:21415355]"
/note="Subunit of the RNA polymerase II mediator complex;
associates with core polymerase subunits to form the RNA
polymerase II holoenzyme; required for basal RNA
polymerase II transcription; homozygosity of the human
MED17 L371P mutation is associated with infantile cerebral
and cerebellar atrophy with poor myelination"
/codon_start=1
/product="Srb4p"
/protein_id="NP_010939.1"
/db_xref="GeneID:856743"
/db_xref="SGD:S000000824"
/translation="MTTEDPDSNHLSSETGIKLALDPNLITLALSSNPNSSLHSPTSD
EPVPESAGKADTSIRLEGDELENKTKKDNDKNLKFLKNKDSLVSNPHEIYGSMPLEQL
IPIILRQRGPGFKFVDLNEKELQNEIKQLGSDSSDGHNSEKKDTDGADENVQIGEDFM
EVDYEDKDNPVDSRNETDHKTNENGETDDNIETVMTQEQFVKRRRDMLEHINLAMNES
SLALEFVSLLLSSVKESTGMSSMSPFLRKVVKPSSLNSDKIPYVAPTKKEYIELDILN
KGWKLQSLNESKDLLRASFNKLSSILQNEHDYWNKIMQSISNKDVIFKIRDRTSGQKL
LAIKYGYEDSGSTYKHDRGIANIRNNIESQNLDLIPHSSSVFKGTDFVHSVKKFLRVR
IFTKIESEDDYILSGESVMDRDSESEEAETKDIRKQIQLLKKIIFEKELMYQIKKECA
LLISYGVSIENENKVIIELPNEKFEIELLSLDDDSIVNHEQDLPKINDKRANLMLVML
RLLLVVIFKKTLRSRISSPHGLINLNVDDDILIIRPILGKVRFANYKLLLKKIIKDYV
LDIVPGSSITETEVEREQPQENKNIDDENITKLNKEIRAFDKLLNIPRRELKINLPLT
EHKSPNLSLMLESPNYCNALIHIKFSAGTEANAVSFDTTFSDFKEVEDFLHFIVAEYI
QQKKV"
gene <201076..>201936
/gene="PRO3"
/locus_tag="YER023W"
/gene_synonym="ORE2"
/db_xref="GeneID:856744"
mRNA <201076..>201936
/gene="PRO3"
/locus_tag="YER023W"
/gene_synonym="ORE2"
/product="pyrroline-5-carboxylate reductase"
/transcript_id="NM_001178914.3"
/db_xref="GeneID:856744"
CDS 201076..201936
/gene="PRO3"
/locus_tag="YER023W"
/gene_synonym="ORE2"
/EC_number="1.5.1.2"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:7009582]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:25112878]"
/experiment="EXISTENCE:mutant phenotype:GO:0004735
pyrroline-5-carboxylate reductase activity
[PMID:1508147|PMID:2824433]"
/experiment="EXISTENCE:mutant phenotype:GO:0055129
L-proline biosynthetic process
[PMID:2824433|PMID:1508147]"
/note="Cytosolic delta 1-pyrroline-5-carboxylate
reductase; catalyzes the last step in proline
biosynthesis"
/codon_start=1
/product="pyrroline-5-carboxylate reductase"
/protein_id="NP_010940.3"
/db_xref="GeneID:856744"
/db_xref="SGD:S000000825"
/translation="MTYTLAILGCGVMGQALLSAIYNAPKAADETAAAFYPSKIITCN
HDEPSAQQVTDLVETFDESPNGIKVESTYGHNVSAVEEASVVLLGTKPFLAEEVLNGV
KSVIGGKLLISLAAGWTIDQLSQYTSTVCRVMTNTPAKYGYGCAVVSYSADVSKEQKP
LVNELISQVGKYVELPEKNMDAATALVGSGPAFVLLMLESLMESGLKLGIPLQESKEC
AMKVLEGTVKMVEKSGAHPSVLKHQVCTPGGTTIAGLCVMEEKGVKSGIINGVEEAAR
VASQLGQKKK"
gene <202192..>204963
/gene="YAT2"
/locus_tag="YER024W"
/db_xref="GeneID:856745"
mRNA <202192..>204963
/gene="YAT2"
/locus_tag="YER024W"
/product="carnitine O-acetyltransferase YAT2"
/transcript_id="NM_001178915.1"
/db_xref="GeneID:856745"
CDS 202192..204963
/gene="YAT2"
/locus_tag="YER024W"
/EC_number="2.3.1.7"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:18427809]"
/experiment="EXISTENCE:mutant phenotype:GO:0004092
carnitine O-acetyltransferase activity [PMID:11329169]"
/experiment="EXISTENCE:mutant phenotype:GO:0006066 alcohol
metabolic process [PMID:11329169]"
/experiment="EXISTENCE:mutant phenotype:GO:0009437
carnitine metabolic process [PMID:11329169]"
/note="Carnitine acetyltransferase; has similarity to
Yat1p, which is a carnitine acetyltransferase associated
with the mitochondrial outer membrane"
/codon_start=1
/product="carnitine O-acetyltransferase YAT2"
/protein_id="NP_010941.1"
/db_xref="GeneID:856745"
/db_xref="SGD:S000000826"
/translation="MSSGSTIVSSDKSGRTFKHEEELPKLPLPKLCDTLQRLKESLEP
LYYADGYYQHPLDPEQIEKLSSIIRDFEENPVSEKLQSKLQSYHDTRDCYLDELHLDI
NNQTSTREIQDDVLPRNPFLVLADDALPNITQADRSAVLVHSAARFISALKQDLLPPD
INATNGKPLSMAPFLNLFGTTRSPVFQRGEVENFDLNKPYTASDLEDPDYSSDEDDND
EPTQKDFDDRKRKHEEDIFTGNGITIKRHPDSKHILIISRGQYYTLEVLDSTNKIIYT
AAELTTIFNHIIKDSSGIEKSTALGSLTSHSFRNWKYARKRLQKRYPNELHRIDSALF
VLVLDESQEETTNDGDDTADISQMFNRTITERDKKCTSANCKRVFYGTSIINSKGHQV
GSCVSRWYDKLQLVVTADAKATVIWDSFTCDGSVVLRFTSEIYTESVLRLARDVNAGD
PQFSLWPNVTQMDPETKKLMTATISADGGGPSEIDPKLVVNKIDWSFSNILNTHVHLS
ETKLADLISKYDIVRASIPLGRRSAQRLGVKPDSMVQVALQIAHYALYGRMVFGLEPV
STRGFKNSRSSFINIQSQALLELCQLFISSSIDGTDKLDKFIQTCETHNNMVKHAKSG
VGYEKHFNALKYLFKFHDHFGIHLSGDESSAAKDLFENPLVLPFSQPELIVANCGNAA
TTTFGITPAVPHGFGIGYIIKDDQVDLTVTSQFRQGDRLMFMLSWVLGEIRSYWRMSR
GTSHNKTGVKISPVVDKLYEMDNAVNNPPKRNGHTVNGSRKTSSSSQVNLNRYGGFFD
LEGHIDSRNISKTPSMKNLQKTFNGLTMSADNDHSSSAVSVPTEKEKLNTGHEILQIQ
PREVASNGLEADDETDIEIVAGNADGTSSSASSATSLNSKKRNVINSRFDIDFDRSRV
GRKVATLDQ"
gene <205251..>206834
/gene="GCD11"
/locus_tag="YER025W"
/gene_synonym="SUI4"
/db_xref="GeneID:856746"
mRNA <205251..>206834
/gene="GCD11"
/locus_tag="YER025W"
/gene_synonym="SUI4"
/product="translation initiation factor eIF2 subunit
gamma"
/transcript_id="NM_001178916.1"
/db_xref="GeneID:856746"
CDS 205251..206834
/gene="GCD11"
/locus_tag="YER025W"
/gene_synonym="SUI4"
/EC_number="3.6.5.3"
/experiment="EXISTENCE:direct assay:GO:0001731 formation
of translation preinitiation complex [PMID:12008673]"
/experiment="EXISTENCE:direct assay:GO:0005525 GTP binding
[PMID:16246727|PMID:14698289]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0016282 eukaryotic
43S preinitiation complex [PMID:12008673]"
/experiment="EXISTENCE:direct assay:GO:0031369 translation
initiation factor binding [PMID:22683627]"
/experiment="EXISTENCE:direct assay:GO:0033290 eukaryotic
48S preinitiation complex [PMID:17242201]"
/experiment="EXISTENCE:direct assay:GO:0043614 multi-eIF
complex [PMID:11018020|PMID:15838098]"
/experiment="EXISTENCE:direct assay:GO:1990856
methionyl-initiator methionine tRNA binding
[PMID:16565414]"
/experiment="EXISTENCE:mutant phenotype:GO:0000049 tRNA
binding [PMID:8947054]"
/experiment="EXISTENCE:mutant phenotype:GO:0003743
translation initiation factor activity [PMID:23063529]"
/experiment="EXISTENCE:mutant phenotype:GO:0005525 GTP
binding [PMID:8947054]"
/experiment="EXISTENCE:mutant phenotype:GO:0005850
eukaryotic translation initiation factor 2 complex
[PMID:8417348]"
/experiment="EXISTENCE:mutant phenotype:GO:0006413
translational initiation [PMID:23063529]"
/experiment="EXISTENCE:mutant phenotype:GO:0045903
positive regulation of translational fidelity
[PMID:9308967]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:22683627]"
/note="Gamma subunit of the translation initiation factor
eIF2; involved in the identification of the start codon;
binds GTP when forming the ternary complex with GTP and
tRNAi-Met; mutations in human ortholog cause X-linked
intellectual disability (XLID) syndrome"
/codon_start=1
/product="translation initiation factor eIF2 subunit
gamma"
/protein_id="NP_010942.1"
/db_xref="GeneID:856746"
/db_xref="SGD:S000000827"
/translation="MSDLQDQEPSIIINGNLEPVGEPDIVEETEVVAQETQETQDADK
PKKKVAFTGLEEDGETEEEKRKREFEEGGGLPEQPLNPDFSKLNPLSAEIINRQATIN
IGTIGHVAHGKSTVVRAISGVQTVRFKDELERNITIKLGYANAKIYKCQEPTCPEPDC
YRSFKSDKEISPKCQRPGCPGRYKLVRHVSFVDCPGHDILMSTMLSGAAVMDAALLLI
AGNESCPQPQTSEHLAAIEIMKLKHVIILQNKVDLMREESALEHQKSILKFIRGTIAD
GAPIVPISAQLKYNIDAVNEFIVKTIPVPPRDFMISPRLIVIRSFDVNKPGAEIEDLK
GGVAGGSILNGVFKLGDEIEIRPGIVTKDDKGKIQCKPIFSNIVSLFAEQNDLKFAVP
GGLIGVGTKVDPTLCRADRLVGQVVGAKGHLPNIYTDIEINYFLLRRLLGVKTDGQKQ
AKVRKLEPNEVLMVNIGSTATGARVVAVKADMARLQLTSPACTEINEKIALSRRIEKH
WRLIGWATIKKGTTLEPIA"
gene 207357..207428
/locus_tag="YNCE0013W"
/db_xref="GeneID:856747"
tRNA 207357..207428
/locus_tag="YNCE0013W"
/product="tRNA-His"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0030371 translation
repressor activity [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043555 regulation
of translation in response to stress [PMID:27609601]"
/note="Histidine tRNA (tRNA-His), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:856747"
/db_xref="SGD:S000006593"
gene complement(<207644..>208474)
/gene="CHO1"
/locus_tag="YER026C"
/gene_synonym="PSS1"
/db_xref="GeneID:856748"
mRNA complement(<207644..>208474)
/gene="CHO1"
/locus_tag="YER026C"
/gene_synonym="PSS1"
/product="CDP-diacylglycerol-serine
O-phosphatidyltransferase"
/transcript_id="NM_001178917.3"
/db_xref="GeneID:856748"
CDS complement(207644..208474)
/gene="CHO1"
/locus_tag="YER026C"
/gene_synonym="PSS1"
/EC_number="2.7.8.8"
/experiment="EXISTENCE:direct assay:GO:0003882
CDP-diacylglycerol-serine O-phosphatidyltransferase
activity [PMID:6088519]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:3005242]"
/experiment="EXISTENCE:direct assay:GO:0006659
phosphatidylserine biosynthetic process [PMID:6088519]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:6274497]"
/experiment="EXISTENCE:mutant phenotype:GO:0003882
CDP-diacylglycerol-serine O-phosphatidyltransferase
activity [PMID:6988386]"
/experiment="EXISTENCE:mutant phenotype:GO:0006659
phosphatidylserine biosynthetic process
[PMID:3031455|PMID:6988386]"
/note="Phosphatidylserine synthase; functions in
phospholipid biosynthesis; transcriptionally repressed by
myo-inositol and choline"
/codon_start=1
/product="CDP-diacylglycerol-serine
O-phosphatidyltransferase"
/protein_id="NP_010943.3"
/db_xref="GeneID:856748"
/db_xref="SGD:S000000828"
/translation="MVESDEDFAPQEFPHTDTDVIVNEHRDENDGYASDEVGGTLSRR
ASSIFSINTTPLAPPNATDIQKFTSDEHHFSMMRNLHMADYITMLNGFSGFYSIVSCL
RFTLTGKPHYVQRAHFFILLGMCFDFLDGRVARLRNRSSLMGQELDSLADLVSFGVAP
AAIAFAIGFQTTFDVMILSFFVLCGLARLARFNVTVAQLPKDSSTGKSKYFEGLPMPT
TLALVLGMAYCVRKGLIFDNIPFGIFREDQILEFHPIILVFFIHGCGMISKSLKIPKP
"
gene complement(<208979..>210232)
/gene="GAL83"
/locus_tag="YER027C"
/gene_synonym="SPM1"
/db_xref="GeneID:856749"
mRNA complement(<208979..>210232)
/gene="GAL83"
/locus_tag="YER027C"
/gene_synonym="SPM1"
/product="Gal83p"
/transcript_id="NM_001178918.1"
/db_xref="GeneID:856749"
CDS complement(208979..210232)
/gene="GAL83"
/locus_tag="YER027C"
/gene_synonym="SPM1"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:17237508|PMID:11331606]"
/experiment="EXISTENCE:direct assay:GO:0005641 nuclear
envelope lumen [PMID:17237508]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:17237508]"
/experiment="EXISTENCE:direct assay:GO:0031588
nucleotide-activated protein kinase complex
[PMID:12393914]"
/experiment="EXISTENCE:genetic interaction:GO:0004679
AMP-activated protein kinase activity [PMID:10990457]"
/experiment="EXISTENCE:genetic interaction:GO:0007165
signal transduction [PMID:10990457]"
/experiment="EXISTENCE:genetic interaction:GO:0043254
regulation of protein-containing complex assembly
[PMID:9121458]"
/experiment="EXISTENCE:mutant phenotype:GO:0001403
invasive growth in response to glucose limitation
[PMID:12556493]"
/experiment="EXISTENCE:mutant phenotype:GO:0004679
AMP-activated protein kinase activity [PMID:7813428]"
/experiment="EXISTENCE:mutant phenotype:GO:0007155 cell
adhesion [PMID:12556493]"
/experiment="EXISTENCE:physical interaction:GO:0031588
nucleotide-activated protein kinase complex
[PMID:7813428|PMID:9121458]"
/note="One of three alternate beta-subunits of the Snf1
kinase complex; allows nuclear localization of the Snf1
kinase complex in the presence of a nonfermentable carbon
source; necessary and sufficient for phosphorylation of
the Mig2p transcription factor in response to alkaline
stress; functionally redundant with SIP1 and SIP2 for the
phosphorylation of Mig1p in response to glucose
deprivation; contains a glycogen-binding domain"
/codon_start=1
/product="Gal83p"
/protein_id="NP_010944.1"
/db_xref="GeneID:856749"
/db_xref="SGD:S000000829"
/translation="MAGDNPENKDASMLDVSDAASNTTINGKHSADSTNEASLAYTFS
QMNVDNPNELEPQHPLRHKSSLIFNDDDDDEIPPYSNHAENGSGETFDSDDDIDASSS
SSIDSNEGDIHDADMTGNTLQKMDYQPSQQPDSLQNQGFQQQQEQQQGTVEGKKGRAM
MFPVDITWQQGGNKVYVTGSFTGWRKMIGLVPVPGQPGLMHVKLQLPPGTHRFRFIVD
NELRFSDYLPTATDQMGNFVNYMEVSAPPDWGNEPQQHLAEKKANHVDDSKLSKRPMS
ARSRIALEIEKEPDDMGDGYTRFHDETPAKPNLEYTQDIPAVFTDPNVMEQYYLTLDQ
QQNNHQNMAWLTPPQLPPHLENVILNSYSNAQTDNTSGALPIPNHVILNHLATSSIKH
NTLCVASIVRYKQKYVTQILYTPLQ"
gene complement(<210692..>211876)
/gene="MIG3"
/locus_tag="YER028C"
/db_xref="GeneID:856750"
mRNA complement(<210692..>211876)
/gene="MIG3"
/locus_tag="YER028C"
/product="Mig3p"
/transcript_id="NM_001178919.1"
/db_xref="GeneID:856750"
CDS complement(210692..211876)
/gene="MIG3"
/locus_tag="YER028C"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:19680226]"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:9832517|PMID:12239282]"
/experiment="EXISTENCE:direct assay:GO:0001227 DNA-binding
transcription repressor activity, RNA polymerase
II-specific [PMID:9832517]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:19158363]"
/experiment="EXISTENCE:genetic interaction:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:19680226]"
/experiment="EXISTENCE:genetic interaction:GO:0001227
DNA-binding transcription repressor activity, RNA
polymerase II-specific [PMID:9832517]"
/experiment="EXISTENCE:genetic interaction:GO:0006974 DNA
damage response [PMID:19680226|PMID:14993292]"
/experiment="EXISTENCE:genetic interaction:GO:0034644
cellular response to UV [PMID:19680226]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:19680226]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:23275883]"
/experiment="EXISTENCE:mutant phenotype:GO:0006974 DNA
damage response [PMID:19680226]"
/experiment="EXISTENCE:mutant phenotype:GO:0045471
response to ethanol [PMID:23275883]"
/note="Transcriptional regulator; partially nonfunctional
in S288C strains but has a major role in catabolite
repression and ethanol response in some other strains;
involved in response to toxic agents; phosphorylation by
Snf1p or the Mec1p pathway inactivates Mig3p, allowing
induction of damage response genes"
/codon_start=1
/product="Mig3p"
/protein_id="NP_010945.1"
/db_xref="GeneID:856750"
/db_xref="SGD:S000000830"
/translation="MNYLRDRFPPDNDQRPFRCEICSRGFHRLEHKKRHGRTHTGEKP
HKCTVQGCPKSFSRSDELKRHLRTHTKGVQRRRIKSKGSRKTVVNTATAAPTTFNENT
GVSLTGIGQSKVPPILISVAQNCDDVNIRNTGNNNGIVETQAPAILVPVINIPNDPHP
IPSSLSTTSITSIASVYPSTSPFQYLKSGFPEDPASTPYVHSSGSSLALGELSSNSSI
FSKSRRNLAAMSGPDSLSSSKNQSSASLLSQTSHPSKSFSRPPTDLSPLRRIMPSVNT
GDMEISRTVSVSSSSSSLTSVTYDDTAAKDMGMGIFFDRPPVTQKACRSNHKYKVNAV
SRGRQHERAQFHISGDDEDSNVHRQESRASNTSPNVSLPPIKSILRQIDNFNSAPSYF
SK"
rep_origin 212383..212631
/note="ARS512; Autonomously Replicating Sequence"
/db_xref="SGD:S000077384"
gene complement(<212587..>213177)
/gene="SMB1"
/locus_tag="YER029C"
/db_xref="GeneID:856751"
mRNA complement(<212587..>213177)
/gene="SMB1"
/locus_tag="YER029C"
/product="mRNA splicing protein SMB1"
/transcript_id="NM_001178920.3"
/db_xref="GeneID:856751"
CDS complement(212587..213177)
/gene="SMB1"
/locus_tag="YER029C"
/experiment="EXISTENCE:direct assay:GO:0005682 U5 snRNP
[PMID:11720284|PMID:11720285]"
/experiment="EXISTENCE:direct assay:GO:0005685 U1 snRNP
[PMID:9630245]"
/experiment="EXISTENCE:direct assay:GO:0046540 U4/U6 x U5
tri-snRNP complex [PMID:10377396|PMID:10449419]"
/experiment="EXISTENCE:direct assay:GO:0071004 U2-type
prespliceosome [PMID:16618970]"
/note="Core Sm protein Sm B; part of heteroheptameric
complex (with Smd1p, Smd2p, Smd3p, Sme1p, Smx3p, and
Smx2p) that is part of the spliceosomal U1, U2, U4, and U5
snRNPs; homolog of human Sm B and Sm B'"
/codon_start=1
/product="mRNA splicing protein SMB1"
/protein_id="NP_010946.3"
/db_xref="GeneID:856751"
/db_xref="SGD:S000000831"
/translation="MSKIQVAHSSRLANLIDYKLRVLTQDGRVYIGQLMAFDKHMNLV
LNECIEERVPKTQLDKLRPRKDSKDGTTLNIKVEKRVLGLTILRGEQILSTVVEDKPL
LSKKERLVRDKKEKKQAQKQTKLRKEKEKKPGKIAKPNTANAKHTSSNSREIAQPSSS
RYNGGNDNIGANRSRFNNEAPPQTRKFQPPPGFKRK"
gene <213437..>213898
/gene="CHZ1"
/locus_tag="YER030W"
/db_xref="GeneID:856752"
mRNA <213437..>213898
/gene="CHZ1"
/locus_tag="YER030W"
/product="Chz1p"
/transcript_id="NM_001178921.1"
/db_xref="GeneID:856752"
CDS 213437..213898
/gene="CHZ1"
/locus_tag="YER030W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095|PMID:17289584]"
/experiment="EXISTENCE:physical interaction:GO:0006338
chromatin remodeling [PMID:17289584]"
/experiment="EXISTENCE:physical interaction:GO:0042393
histone binding [PMID:17289584]"
/note="Histone chaperone for Htz1p/H2A-H2B dimer; required
for the stabilization of the Chz1p-Htz1-H2B complex; has
overlapping function with Nap1p; null mutant displays weak
sensitivity to MMS and benomyl; contains a highly
conserved CHZ motif; protein abundance increases in
response to DNA replication stress"
/codon_start=1
/product="Chz1p"
/protein_id="NP_010947.2"
/db_xref="GeneID:856752"
/db_xref="SGD:S000000832"
/translation="MSDEAKEKRELESQKESSHNKSEKSVEPKPKRRRRRNYDDYDAE
VAKEETKAKNGLTKSENNGTVEDSESDMDDAKLDALMGNEGEEEEDDLAEIDTSNIIT
SGRRTRGKVIDYKKTAEELDKKEPSTGSKDDVGYGEKEEDDEDEEDDDFKE"
gene complement(<214076..>214747)
/gene="YPT31"
/locus_tag="YER031C"
/gene_synonym="YPT8"
/db_xref="GeneID:856753"
mRNA complement(<214076..>214747)
/gene="YPT31"
/locus_tag="YER031C"
/gene_synonym="YPT8"
/product="Rab family GTPase YPT31"
/transcript_id="NM_001178922.1"
/db_xref="GeneID:856753"
CDS complement(214076..214747)
/gene="YPT31"
/locus_tag="YER031C"
/gene_synonym="YPT8"
/experiment="EXISTENCE:direct assay:GO:0003924 GTPase
activity [PMID:11210571]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:16407407]"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:15537705]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:15537705]"
/experiment="EXISTENCE:direct assay:GO:0005802 trans-Golgi
network [PMID:26906739]"
/experiment="EXISTENCE:genetic interaction:GO:0034498
early endosome to Golgi transport [PMID:15537705]"
/experiment="EXISTENCE:mutant phenotype:GO:0006887
exocytosis [PMID:9151665]"
/experiment="EXISTENCE:mutant phenotype:GO:0016192
vesicle-mediated transport [PMID:9151665]"
/note="Rab family GTPase; involved in the exocytic
pathway; mediates intra-Golgi traffic or the budding of
post-Golgi vesicles from the trans-Golgi; YPT31 has a
paralog, YPT32, that arose from the whole genome
duplication; localizes to the transitional and late Golgi"
/codon_start=1
/product="Rab family GTPase YPT31"
/protein_id="NP_010948.1"
/db_xref="GeneID:856753"
/db_xref="SGD:S000000833"
/translation="MSSEDYGYDYDLLFKIVLIGDSGVGKSNLLSRFTKNEFNMDSKS
TIGVEFATRTLEIDGKRIKAQIWDTAGQERYRAITSAYYRGAVGALIVYDISKSSSYE
NCNHWLSELRENADDNVAVGLIGNKSDLAHLRAVPTEESKTFAQENQLLFTETSALNS
ENVDKAFEELINTIYQKVSKHQMDLGDSSANGNANGASAPNGPTISLTPTPNENKKAN
GNNCC"
gene <215063..>217693
/gene="FIR1"
/locus_tag="YER032W"
/gene_synonym="PIP1"
/db_xref="GeneID:856754"
mRNA <215063..>217693
/gene="FIR1"
/locus_tag="YER032W"
/gene_synonym="PIP1"
/product="Fir1p"
/transcript_id="NM_001178923.3"
/db_xref="GeneID:856754"
CDS 215063..217693
/gene="FIR1"
/locus_tag="YER032W"
/gene_synonym="PIP1"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0032465
regulation of cytokinesis [PMID:30455324]"
/note="Protein involved in 3' mRNA processing; interacts
with Ref2p; APCC(Cdh1) substrate; potential Cdc28p
substrate"
/codon_start=1
/product="Fir1p"
/protein_id="NP_010949.4"
/db_xref="GeneID:856754"
/db_xref="SGD:S000000834"
/translation="MSLPVTPVKSKVCSTLSIQHEMDHDQCRDVLCPRENLLAINRTN
NIESVAIPRQRSSKNKKPHEHTQSQVRFSIPDPNEISQDSPLKIVFPKSGNETERRMS
TSSLLMNSHGHLVDMHSKILVDVPKEVWKFHHNRRKKCESRHRKTRSDVRSNTSSSGK
EPNHSRSKSLQSIIVDTMNTYRATEVETSINENTSNISQVSPLNLSFDRPPPLTPEKN
LYLTPESPLNRYHLPVPLEISLPPYLSPQNKDKKRSSLVYDGDGYSQFQEGNTSSSTE
SSLEQPSSSYSDEDDSIPYAHHDVSFELNNADADKLLGIDENANVNLKIQRRNLKNPQ
HIKSKTDRECEEKNTEKNVSLKILSTPNKLIDIPDLEHMKSPPSTGLNGTLKFFQQFE
PSEEPTSPTRQVNPESLDKLDMSFKFPSSTTNNNVDKVHENRNSGDTNNEDFLKVDTS
PVNQSFESRRQMLIDLQKSPTNNNPRIHKHRRSRSVHNIDDISLNFEATSTPPAPTSA
PSIPVEHSNPCTSIEIPKRSPLRFTSSPKTSDIPPEAQSPKNGSFLQEISVPSIQIIP
DESISHTREPSPSLIECPEDENEAFSTEVADHSIAIISETKSVPSIEPFKPLSSFNSF
GQEIQNKEPTPLNQTPTDLIGKQRNCVNPHSIPFSVLSSNSQSSQSGSSKSSYNSEFS
SNTAITDTTSQPSVTINRSTLEHNFIEEKRSIKNLGHGPPSQKNNYSFPRNKNTPSNR
HDLDFNTIYEKRDGKMVEVILLDEDEDVGLKNNDISRTRVCHAQKAKNEQQKKRLSHC
NEILGMCDKTADDAKRIIYQLVNEKHKFSEKGQQTKPKKSRVLPPLPFPLYDEKGNSL
IPNKYQSSIHNDIPSHRKLK"
gene complement(<218057..>221287)
/gene="ZRG8"
/locus_tag="YER033C"
/db_xref="GeneID:856755"
mRNA complement(<218057..>221287)
/gene="ZRG8"
/locus_tag="YER033C"
/product="Zrg8p"
/transcript_id="NM_001178924.1"
/db_xref="GeneID:856755"
CDS complement(218057..221287)
/gene="ZRG8"
/locus_tag="YER033C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005933 cellular
bud [PMID:15972461]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:15972461]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:15972461]"
/experiment="EXISTENCE:mutant phenotype:GO:0031505
fungal-type cell wall organization [PMID:15972461]"
/note="hypothetical protein; authentic, non-tagged protein
is detected in highly purified mitochondria in
high-throughput studies; GFP-fusion protein is localized
to the cytoplasm; transcription induced under conditions
of zinc deficiency"
/codon_start=1
/product="Zrg8p"
/protein_id="NP_010950.1"
/db_xref="GeneID:856755"
/db_xref="SGD:S000000835"
/translation="MRSFIKAHKKSTSFDESPKRHSNFSGNTNNSSQRSSDDSLDFLP
STPSQMNYDSIPPPAKHSPGFESFHRLANKTSKLFKKTSNSNLNSHLASTPTTSTNQT
TSNSFVLQNPPTKNTGPPPPLPPPLFPSSSTSSFSRHDNESEYTAYKKTSPAKDFNRT
TDSLPAIKGTITHSWGDSKVESHVIILNDPASPASNTSEATSSKQFKTPIIGNENLTS
TTSPSNLEPAIRILNKNKGKQQENIDDAEDGSSKKEHHVYKALALAKNRNRQARIHSH
DDIINLGKASQMDMSLLAAAFSGNSTTTINNDQSSNEQTDEKILDIERVTTTSTLTSS
ETTSPINKSPCFYSQTLSLSPKIRHGDLQSSPSKVNKNDSQNETLNKKKVRISLNRKE
EEKVYSLNNNSDEYSVNEKETHKANDCNDESSENGDGDNDHDDDYDDDDDDDDDDDES
EFSFEYAGINVRTSSVKYYSKPEPAANVYIDDLYEDENFDDDMNCIEDDESGNEGNEI
CGLSTRFEETSLKSNKVKKFNDLFNLSDDDEEEDGKDNSNNGDENESDNLYQKRLENG
KETFNGNHGGHHDDASLGETVDNKEQFLINDNVKKPIQKYNDLFDLSDEDDNDDKEMS
EAESYMFSDEAPSIESGPANAKSTRGIYSQSNKNIIRDGKPNYSFSLKRNNSDDETEH
TSAIKASLTGTTGSTKPTVKSFSDIFNVDDSASDAESDSGTGGNNSNGLVSNDSERQV
SLQSSLYETKSESHPPNHPHSQILQTPAKIVITPSVSDAQSQALAITDDDGEDDDDDT
SSILRTPFQLIDSSHSQQPHYASPQYTAVLNSPPLPPPARSQSLKYHDLNCDLDSEVP
RPMSNLFFIDEAEEDEYNQKSKFFDFDHYDIDEINGIPEDFNFSDSERDDLNRRTLKS
PLRGGSKNREVSPFSSVSSSFRSTHSFNGKLTINQGAKELAPMKNKIELTNKTVTFFN
SNNWNTYDCNSLSRKTSSQMRDSKYQNHNVGQNVEPSSVLSPQHQISNGLDGKCNDNY
VISPNLPTTITPTNSFTKPTPEFSNDYSLSPIQETPSSVQSSPKRA"
gene <221846..>222403
/locus_tag="YER034W"
/db_xref="GeneID:856756"
mRNA <221846..>222403
/locus_tag="YER034W"
/product="uncharacterized protein"
/transcript_id="NM_001178925.1"
/db_xref="GeneID:856756"
CDS 221846..222403
/locus_tag="YER034W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="hypothetical protein; non-essential gene;
expression induced upon calcium shortage; protein
abundance increases in response to DNA replication stress"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_010951.1"
/db_xref="GeneID:856756"
/db_xref="SGD:S000000836"
/translation="MDAFSLKKDNRKKFQDKQKLKRKHATPSDRKYRLLNRQKEEKAT
TEEKDQDQEQPALKSNEDRYYEDPVLEDPHSAVANAELNKVLKDVLKNRLQQNDDATA
VNNVANKDTLKIKDLKQMNTDELNRWLGRQNTTSAITAAEPESLVVPIHVQGDHDRAG
KKISAPSTDLPEELETDQDFLDGLL"
gene <222639..>223076
/gene="EDC2"
/locus_tag="YER035W"
/db_xref="GeneID:856757"
mRNA <222639..>223076
/gene="EDC2"
/locus_tag="YER035W"
/product="Edc2p"
/transcript_id="NM_001178926.3"
/db_xref="GeneID:856757"
CDS 222639..223076
/gene="EDC2"
/locus_tag="YER035W"
/experiment="EXISTENCE:direct assay:GO:0000290
deadenylation-dependent decapping of nuclear-transcribed
mRNA [PMID:12554867]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:12554867]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095|PMID:19682251|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:19682251]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922|PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0032056
positive regulation of translation in response to stress
[PMID:19682251]"
/note="RNA-binding protein that directly activates mRNA
decapping; binds mRNA substrate and enhances activity of
decapping proteins Dcp1p and Dcp2p; has a role in
translation during heat stress; protein increases in
abundance and relocalizes to nucleolus and to nuclear foci
upon DNA replication stress; EDC2 has a paralog, EDC1,
that arose from the whole genome duplication"
/codon_start=1
/product="Edc2p"
/protein_id="NP_010952.3"
/db_xref="GeneID:856757"
/db_xref="SGD:S000000837"
/translation="MGSETKHSAKVKIVTRESPPSAKEHMRPTKTQILVPPTQSLPNG
KKPNFGKSTKQRREPRERTSKTGHEDDKATMVTVNIDAFLHDKAPKKKSCKYKKKKTR
QYQDRAAASIDSKPHVAGHTAFAGASFTTDIPHEAALPKPSFV"
gene complement(<223367..>225199)
/gene="ARB1"
/locus_tag="YER036C"
/db_xref="GeneID:856758"
mRNA complement(<223367..>225199)
/gene="ARB1"
/locus_tag="YER036C"
/product="ATP-binding cassette family ATPase ARB1"
/transcript_id="NM_001178927.1"
/db_xref="GeneID:856758"
CDS complement(223367..225199)
/gene="ARB1"
/locus_tag="YER036C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:16260602]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0000056
ribosomal small subunit export from nucleus
[PMID:16260602]"
/experiment="EXISTENCE:mutant phenotype:GO:0016887 ATP
hydrolysis activity [PMID:16260602]"
/experiment="EXISTENCE:mutant phenotype:GO:0042254
ribosome biogenesis [PMID:16260602]"
/experiment="EXISTENCE:mutant phenotype:GO:0043335 protein
unfolding [PMID:32320318]"
/note="ATPase of the ATP-binding cassette (ABC) family;
involved in 40S and 60S ribosome biogenesis, has
similarity to Gcn20p; important for disaggregation of
ordered and disordered aggregates; shuttles from nucleus
to cytoplasm, physically interacts with Tif6p, Lsg1p;
human homolog ABCF2 can complement yeast ARB1 mutant"
/codon_start=1
/product="ATP-binding cassette family ATPase ARB1"
/protein_id="NP_010953.1"
/db_xref="GeneID:856758"
/db_xref="SGD:S000000838"
/translation="MPPVSASKAKRDAKKAEREAKKAAAGKTIRKLGRKKEAAAEESE
VDAAAREIKMMKLQQDKDGLSDRVVTGVLSSLETSRDIKLSSVSLLFHGKVLIQDSGL
ELNYGRRYGLLGENGCGKSTFLKALATREYPIPEHIDIYLLDEPAEPSELSALDYVVT
EAQHELKRIEDLVEKTILEDGPESELLEPLYERMDSLDPDTFESRAAIILIGLGFNKK
TILKKTKDMSGGWKMRVALAKALFVKPTLLLLDDPTAHLDLEACVWLEEYLKRFDRTL
VLVSHSQDFLNGVCTNMIDMRAQKLTAYGGNYDSYHKTRSELETNQMKQYNKQQEEIQ
HIKKFIASAGTYANLVKQAKSRQKILDKMEADGLVQPVVPDKVFSFRFPQVERLPPPV
LAFDDISFHYESNPSENLYEHLNFGVDMDSRIALVGPNGVGKSTLLKIMTGELTPQSG
RVSRHTHVKLGVYSQHSQDQLDLTKSALEFVRDKYSNISQDFQFWRGQLGRYGLTGEG
QTVQMATLSEGQRSRVVFALLALEQPNVLLLDEPTNGLDIPTIDSLADAINEFNGGVV
VVSHDFRLLDKIAQDIFVVENKTATRWDGSILQYKNKLAKNVVL"
gene <225889..>226854
/gene="PHM8"
/locus_tag="YER037W"
/db_xref="GeneID:856759"
mRNA <225889..>226854
/gene="PHM8"
/locus_tag="YER037W"
/product="bifunctional nucleotidase/lysophosphatidic acid
phosphatase"
/transcript_id="NM_001178928.1"
/db_xref="GeneID:856759"
CDS 225889..226854
/gene="PHM8"
/locus_tag="YER037W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008252
nucleotidase activity [PMID:23670538]"
/experiment="EXISTENCE:direct assay:GO:0052642
lysophosphatidic acid phosphatase activity
[PMID:18234677]"
/experiment="EXISTENCE:mutant phenotype:GO:0009166
nucleotide catabolic process [PMID:23670538]"
/experiment="EXISTENCE:mutant phenotype:GO:0016036
cellular response to phosphate starvation [PMID:18234677]"
/experiment="EXISTENCE:mutant phenotype:GO:0052642
lysophosphatidic acid phosphatase activity
[PMID:18234677]"
/experiment="EXISTENCE:mutant phenotype:GO:0070328
triglyceride homeostasis [PMID:26979516]"
/experiment="EXISTENCE:mutant phenotype:GO:0090549
response to carbon starvation [PMID:23670538]"
/note="Lysophosphatidic acid (LPA) phosphatase,
nucleotidase; principle and physiological nucleotidase
working on GMP, UMP and CMP; involved in LPA hydrolysis in
response to phosphate starvation and ribose salvage
pathway; phosphatase activity is soluble and Mg2+
dependent; expression is induced by low phosphate levels
and by inactivation of Pho85p; repressed by Gcn4p under
normal conditions; PHM8 has a paralog, SDT1, that arose
from the whole genome duplication"
/codon_start=1
/product="bifunctional nucleotidase/lysophosphatidic acid
phosphatase"
/protein_id="NP_010954.1"
/db_xref="GeneID:856759"
/db_xref="SGD:S000000839"
/translation="MTIAKDYRTIYRNQIKKQIRLNQEHLQSLTHLGSQINFEVDPPK
LPDPDPARKVFFFDIDNTLYRKSTKVQLLMQQSLSNFFKYELGFDDDEAERLIESYYQ
EYGLSVKGLIKNKQIDDVLQYNTFIDDSLPLQDYLKPDWKLRELLINLKKKKLGKFDK
LWLFTNSYKNHAIRCVKILGIADLFDGITYCHYDRPIEEEFICKPDPKFFETAKLQSG
LSSFANAWFIDDNESNVRSALSMGMGHVIHLIEDYQYESENIVTKDHKNKQQFSILKD
ILEIPLIMDVEVYRPSSIAIKEMEELEEEGEAVNWSNQQINVQSS"
gene complement(<226858..>228252)
/gene="KRE29"
/locus_tag="YER038C"
/gene_synonym="NSE6"
/db_xref="GeneID:856760"
mRNA complement(<226858..>228252)
/gene="KRE29"
/locus_tag="YER038C"
/gene_synonym="NSE6"
/product="Smc5-Smc6 complex subunit KRE29"
/transcript_id="NM_001178929.3"
/db_xref="GeneID:856760"
CDS complement(226858..228252)
/gene="KRE29"
/locus_tag="YER038C"
/gene_synonym="NSE6"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0019789 SUMO
transferase activity [PMID:15738391]"
/experiment="EXISTENCE:direct assay:GO:0030915 Smc5-Smc6
complex [PMID:15738391]"
/experiment="EXISTENCE:direct assay:GO:0042030 ATPase
inhibitor activity [PMID:34191293]"
/experiment="EXISTENCE:mutant phenotype:GO:0006281 DNA
repair [PMID:15738391]"
/note="Subunit of the SMC5-SMC6 complex; this complex is
involved in removal of X-shaped DNA structures that arise
between sister chromatids during DNA replication and
repair; heterozygous mutant shows haploinsufficiency in K1
killer toxin resistance"
/codon_start=1
/product="Smc5-Smc6 complex subunit KRE29"
/protein_id="NP_010955.3"
/db_xref="GeneID:856760"
/db_xref="SGD:S000000840"
/translation="MGSVNSSPNEEFETVPDSQISGFDSPLIPTSVGSYFRDDDDDEK
VHPNFISDPENDSLNSDEEFSSLENSDLNLSGAKAESGDDFDPILKRTIISKRKAPSN
NEDEEIVKTPRKLVNYVPLKIFNLGDSFDDTITTTVAKLQDLKKEILDSPRSNKSIVI
TSNTVAKSELQKSIKFSGSIPEIYLDVVTKETISDKYKDWHFISKNCHYEQLMDLEMK
DTAYSFLFGSSRSQGKVPEFVHLKCPSITNLLVLFGVNQEKCNSLKINYEKKENSRYD
NLCTIFPVNKMLKFLMYFYSDDDNDDVREFFLKAFICLILDRKVFNAMESDHRLCFKV
LELFNEAHFINSYFEIVDKNDFFLHYRLLQIFPHLQSALLRRRFSEKQGRTETIQQNI
IKEFNEFFDCKNYKNLLYFILTMYGSKFIPFGPKCQVTEYFKDCILDISNETTNDVEI
SILKGILNLFSKIR"
gene <228451..>228831
/gene="FMP49"
/locus_tag="YER038W-A"
/db_xref="GeneID:20098144"
mRNA <228451..>228831
/gene="FMP49"
/locus_tag="YER038W-A"
/product="Fmp49p"
/transcript_id="NM_001301348.1"
/db_xref="GeneID:20098144"
CDS 228451..228831
/gene="FMP49"
/locus_tag="YER038W-A"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/note="Mitochondrial hypothetical protein; almost
completely overlaps ORF HVG1/YER039C"
/codon_start=1
/product="Fmp49p"
/protein_id="NP_001288277.1"
/db_xref="GeneID:20098144"
/db_xref="SGD:S000028746"
/translation="MYYFSRVAARTFCCCIFFCLATAYSRPDRNPRKIEKKDKKFFGA
SKNTNPANAMGNLFKAPTIEYVVEEVTRTHQPEQYDIPTDMSPLMTIAASESADKFTD
KFFVDQSSIMKEKTSSKGNARTLL"
gene complement(<228456..>229205)
/gene="HVG1"
/locus_tag="YER039C"
/gene_synonym="YEM9"
/db_xref="GeneID:856761"
mRNA complement(<228456..>229205)
/gene="HVG1"
/locus_tag="YER039C"
/gene_synonym="YEM9"
/product="putative GDP-mannose transporter"
/transcript_id="NM_001178930.1"
/db_xref="GeneID:856761"
CDS complement(228456..229205)
/gene="HVG1"
/locus_tag="YER039C"
/gene_synonym="YEM9"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/note="hypothetical protein; HVG1 has a paralog, VRG4,
that arose from the whole genome duplication"
/codon_start=1
/product="putative GDP-mannose transporter"
/protein_id="NP_010956.1"
/db_xref="GeneID:856761"
/db_xref="SGD:S000000841"
/translation="MIYTSSKSLQYLAVPIYTIFKNLTIILIAYGEVLFFGGKVTSME
LTSFIMMVLSSVVATWGDQQAIAIKASSLEDLDQELVESTIFVLNPGYLWMFTNCISS
ALFVLIMRKRIRLTNFKDYDTMFYNNVLALPLLLVFSFIMEDWSTKNLSVNLSADSLA
AMVISGLMSVGISYCSGWCVRVTSSTTYSMVGALNKLPIALAGLVFFDAPKNFLSFFS
IFLGFLSGLLYAVAKQKKIQQQKVLAATLEK"
gene complement(<229263..>229481)
/locus_tag="YER039C-A"
/db_xref="GeneID:856762"
mRNA complement(<229263..>229481)
/locus_tag="YER039C-A"
/product="uncharacterized protein"
/transcript_id="NM_001184319.1"
/db_xref="GeneID:856762"
CDS complement(229263..229481)
/locus_tag="YER039C-A"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:40704575]"
/note="hypothetical protein; YER039C-A is not an essential
gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_010957.1"
/db_xref="GeneID:856762"
/db_xref="SGD:S000007226"
/translation="MSKHKHEWTESVANSGPASILSYCASSILMTVTNKFVVNLDNFN
MNFVMLFVQSLVCTVTLCILRIVGVANF"
gene <229795..>231987
/gene="GLN3"
/locus_tag="YER040W"
/db_xref="GeneID:856763"
mRNA <229795..>231987
/gene="GLN3"
/locus_tag="YER040W"
/product="nitrogen-responsive transcriptional regulator
GLN3"
/transcript_id="NM_001178931.3"
/db_xref="GeneID:856763"
CDS 229795..231987
/gene="GLN3"
/locus_tag="YER040W"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:7608102]"
/experiment="EXISTENCE:direct assay:GO:0003700 DNA-binding
transcription factor activity [PMID:8655543]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10604478|PMID:27293098]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:12140287]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:19111667]"
/experiment="EXISTENCE:mutant phenotype:GO:0003700
DNA-binding transcription factor activity [PMID:8655543]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:2153652|PMID:7568152]"
/note="Transcriptional activator in nitrogen catabolite
repression system; localization and activity regulated by
quality of nitrogen source and Ure2p; full-length protein
forms prion-like aggregates when overproduced"
/codon_start=1
/product="nitrogen-responsive transcriptional regulator
GLN3"
/protein_id="NP_010958.3"
/db_xref="GeneID:856763"
/db_xref="SGD:S000000842"
/translation="MQDDPENSKLYDLLNSHLDVHGRSNEEPRQTGDSRSQSSGNTGE
NEEDIAFASGLNGGTFDSMLEALPDDLYFTDFVSPFTAAATTSVTTKTVKDTTPATNH
MDDDIAMFDSLATTQPIDIAASNQQNGEIAQLWDFNVDQFNMTPSNSSGSATISAPNS
FTSDIPQYNHGSLGNSVSKSSLFPYNSSTSNSNINQPSINNNSNTNAQSHHSFNIYKL
QNNNSSSSAMNITNNNNSNNSNIQHPFLKKSDSIGLSSSNTTNSVRKNSLIKPMSSTS
LANFKRAASVSSSISNMEPSGQNKKPLIQCFNCKTFKTPLWRRSPEGNTLCNACGLFQ
KLHGTMRPLSLKSDVIKKRISKKRAKQTDPNIAQNTPSAPATASTSVTTTNAKPIRSR
KKSLQQNSLSRVIPEEIIRDNIGNTNNILNVNRGGYNFNSVPSPVLMNSQSYNSSNAN
FNGASNANLNSNNLMRHNSNTVTPNFRRSSRRSSTSSNTSSSSKSSSRSVVPILPKPS
PNSANSQQFNMNMNLMNTTNNVSAGNSVASSPRIISSANFNSNSPLQQNLLSNSFQRQ
GMNIPRRKMSRNASYSSSFMAASLQQLHEQQQVDVNSNTNTNSNRQNWNSSNSVSTNS
RSSNFVSQKPNFDIFNTPVDSPSVSRPSSRKSHTSLLSQQLQNSESNSFISNHKFNNR
LSSDSTSPIKYEADVSAGGKISEDNSTKGSSKESSAIADELDWLKFGI"
gene <232461..>234740
/gene="YEN1"
/locus_tag="YER041W"
/db_xref="GeneID:856764"
mRNA <232461..>234740
/gene="YEN1"
/locus_tag="YER041W"
/product="crossover junction endodeoxyribonuclease"
/transcript_id="NM_001178932.2"
/db_xref="GeneID:856764"
CDS 232461..234740
/gene="YEN1"
/locus_tag="YER041W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:34928393|PMID:19520826]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:19520826]"
/experiment="EXISTENCE:direct assay:GO:0006281 DNA repair
[PMID:34928393]"
/experiment="EXISTENCE:direct assay:GO:0008821 crossover
junction DNA endonuclease activity [PMID:19020614]"
/experiment="EXISTENCE:direct assay:GO:0051908
double-stranded DNA 5'-3' DNA exonuclease activity
[PMID:34928393]"
/experiment="EXISTENCE:genetic interaction:GO:0006281 DNA
repair [PMID:20106725]"
/experiment="EXISTENCE:genetic interaction:GO:0006974 DNA
damage response [PMID:20106725]"
/experiment="EXISTENCE:mutant phenotype:GO:0008821
crossover junction DNA endonuclease activity
[PMID:19020614]"
/note="Holliday junction resolvase; promotes template
switching during break-induced replication (BIR), causing
non-reciprocal translocations (NRTs); localization is
cell-cycle dependent and regulated by Cdc28p
phosphorylation; homolog of human GEN1; similar to S.
cerevisiae endonuclease Rth1p"
/codon_start=1
/product="crossover junction endodeoxyribonuclease"
/protein_id="NP_010959.2"
/db_xref="GeneID:856764"
/db_xref="SGD:S000000843"
/translation="MGVSQIWEFLKPYLQDSRIPLRKFVIDFNKSQKRAPRIAIDAYG
WLFECGFIQNIDISARSRSRSRSPTRSPRDSDIDSSQEYYGSRSYTTTGKAVINFISR
LKELLSLNVEFLLVFDGVMKPSFKRKFNHEQNATTCDDEKEYYSSWEQHVKNHEVYGN
CKGLLAPSDPEFISLVRKLLDLMNISYVIACGEGEAQCVWLQVSGAVDFILSNDSDTL
VFGGEKILKNYSKFYDDFGPSSITSHSPSRHHDSKESFVTVIDLPKINKVAGKKFDRL
SLLFFSVLLGADYNRGVKGLGKNKSLQLAQCEDPNFSMEFYDIFKDFNLEDLTSESLR
KSRYRLFQKRLYLYCKDHSVELFGRNYPVLLNQGSFEGWPSTVAIMHYFHPIVQPYFD
EEVLSDKYINMAGNGHYRNLNFNELKYFLQSLNLPQISSFDKWFHDSMHEMFLLREFL
SIDESDNIGKGNMRITEEKIMNIDGGKFQIPCFKIRYTTFLPNIPISSQSPLKRSNSP
SRSKSPTRRQMDIMEHPNSLWLPKYLIPQSHPLVIQYYETQQLIQKEKEKKGKKSNKS
RLPQKNNLDEFLRKHTSPIKSIGKVGESRKEILEPVRKRLFVDTDEDTSLEEIPAPTR
LTTVDEHSDNDDDSLIFVDEITNSQSVLDSSPGKRIRDLTQDEQVDVWKDVIEISPIK
KSRTTNAEKNPPESGLKSRSSITINARLQGTKMLPPNLTAPRLEREHSSVLDQLVTDA
QDTVDRFVACDSDSSSTIE"
gene <234937..>235491
/gene="MXR1"
/locus_tag="YER042W"
/db_xref="GeneID:856765"
mRNA <234937..>235491
/gene="MXR1"
/locus_tag="YER042W"
/product="peptide-methionine-S-sulfoxide reductase"
/transcript_id="NM_001178933.1"
/db_xref="GeneID:856765"
CDS 234937..235491
/gene="MXR1"
/locus_tag="YER042W"
/EC_number="1.8.4.11"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:20799725]"
/experiment="EXISTENCE:direct assay:GO:0008113
peptide-methionine (S)-S-oxide reductase activity
[PMID:15141092|PMID:9275166]"
/experiment="EXISTENCE:genetic interaction:GO:0034599
cellular response to oxidative stress [PMID:11929995]"
/experiment="EXISTENCE:mutant phenotype:GO:0008113
peptide-methionine (S)-S-oxide reductase activity
[PMID:9275166|PMID:15141092]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress
[PMID:11929995|PMID:9275166]"
/note="Methionine-S-sulfoxide reductase; involved in the
response to oxidative stress; protects iron-sulfur
clusters from oxidative inactivation along with MXR2;
involved in the regulation of lifespan; reduced activity
of human homolog implicated in Alzheimer disease"
/codon_start=1
/product="peptide-methionine-S-sulfoxide reductase"
/protein_id="NP_010960.1"
/db_xref="GeneID:856765"
/db_xref="SGD:S000000844"
/translation="MSSLISKTIKYDPAKDKLITLACGCFWGTEHMYRKYLNDRIVDC
KVGYANGEESKKDSPSSVSYKRVCGGDTDFAEVLQVSYNPKVITLRELTDFFFRIHDP
TTSNSQGPDKGTQYRSGLFAHSDADLKELAKIKEEWQPKWGNKIATVIEPIKNFYDAE
EYHQLYLDKNPQGYACPTHYLREM"
gene complement(<235770..>237119)
/gene="SAH1"
/locus_tag="YER043C"
/db_xref="GeneID:856766"
mRNA complement(<235770..>237119)
/gene="SAH1"
/locus_tag="YER043C"
/product="adenosylhomocysteinase"
/transcript_id="NM_001178934.3"
/db_xref="GeneID:856766"
CDS complement(235770..237119)
/gene="SAH1"
/locus_tag="YER043C"
/EC_number="3.13.2.1"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0004013
adenosylhomocysteinase activity [PMID:18591246]"
/experiment="EXISTENCE:mutant phenotype:GO:0006641
triglyceride metabolic process [PMID:18591246]"
/experiment="EXISTENCE:mutant phenotype:GO:0006656
phosphatidylcholine biosynthetic process [PMID:18591246]"
/experiment="EXISTENCE:mutant phenotype:GO:0033353
S-adenosylmethionine cycle [PMID:18591246]"
/note="S-adenosyl-L-homocysteine hydrolase; catabolizes
S-adenosyl-L-homocysteine which is formed after donation
of the activated methyl group of S-adenosyl-L-methionine
(AdoMet) to an acceptor; regulates cellular lipid
homoeostasis by regulating
phosphatidylcholine(PC)synthesis and triacylglycerol (TG)
levels"
/codon_start=1
/product="adenosylhomocysteinase"
/protein_id="NP_010961.3"
/db_xref="GeneID:856766"
/db_xref="SGD:S000000845"
/translation="MSAPAQNYKIADISLAAFGRKEIELAEHEMPGLMAIRKAYGDVQ
PLKGARIAGCLHMTIQTAVLIETLVALGAEVTWSSCNIYSTQDHAAAAIAASGVPVFA
WKGETEEEYLWCIEQQLFAFKDNKKLNLILDDGGDLTTLVHEKHPEMLEDCFGLSEET
TTGVHHLYRMVKEGKLKVPAINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMLAGKV
AVVAGYGDVGKGCAAALRGMGARVLVTEIDPINALQAAMEGYQVVTMEDASHIGQVFV
TTTGCRDIINGEHFINMPEDAIVCNIGHFDIEIDVAWLKANAKECINIKPQVDRYLLS
SGRHVILLANGRLVNLGCATGHSSFVMSCSFSNQVLAQIALFKSNDKSFREKHIEFQK
TGPFEVGVHVLPKILDEAVAKFHLGNLGVRLTKLSKVQSEYLGIPEEGPFKADHYRY"
gene complement(<237570..>238016)
/gene="ERG28"
/locus_tag="YER044C"
/gene_synonym="BUD18"
/db_xref="GeneID:856767"
mRNA complement(<237570..>238016)
/gene="ERG28"
/locus_tag="YER044C"
/gene_synonym="BUD18"
/product="Erg28p"
/transcript_id="NM_001178935.1"
/db_xref="GeneID:856767"
CDS complement(237570..238016)
/gene="ERG28"
/locus_tag="YER044C"
/gene_synonym="BUD18"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:12119386]"
/experiment="EXISTENCE:direct assay:GO:0006696 ergosterol
biosynthetic process [PMID:11160377]"
/experiment="EXISTENCE:mutant phenotype:GO:0006696
ergosterol biosynthetic process
[PMID:29319811|PMID:10929718]"
/experiment="EXISTENCE:physical interaction:GO:0030674
protein-macromolecule adaptor activity
[PMID:12119386|PMID:15522820]"
/note="Endoplasmic reticulum membrane protein; may
facilitate protein-protein interactions between the Erg26p
dehydrogenase and the Erg27p 3-ketoreductase and/or tether
these enzymes to the ER, also interacts with Erg6p"
/codon_start=1
/product="Erg28p"
/protein_id="NP_010962.1"
/db_xref="GeneID:856767"
/db_xref="SGD:S000000846"
/translation="MFSLQDVITTTKTTLAAMPKGYLPKWLLFISIVSVFNSIQTYVS
GLELTRKVYERKPTETTHLSARTFGTWTFISCVIRFYGAMYLNEPHIFELVFMSYMVA
LFHFGSELLIFRTCKLGKGFMGPLVVSTTSLVWMYKQREYYTGVAW"
gene complement(<238460..>239774)
/gene="MEI4"
/locus_tag="YER044C-A"
/db_xref="GeneID:856768"
mRNA complement(join(<238460..239623,239712..>239774))
/gene="MEI4"
/locus_tag="YER044C-A"
/product="Mei4p"
/transcript_id="NM_001180023.1"
/db_xref="GeneID:856768"
CDS complement(join(238460..239623,239712..239774))
/gene="MEI4"
/locus_tag="YER044C-A"
/experiment="EXISTENCE:direct assay:GO:0000794 condensed
nuclear chromosome [PMID:17558514|PMID:16783010]"
/experiment="EXISTENCE:genetic interaction:GO:0042138
meiotic DNA double-strand break formation [PMID:10224247]"
/note="Meiosis-specific protein involved in forming DSBs;
involved in double-strand break (DSBs) formation during
meiotic recombination; required for chromosome synapsis
and production of viable spores"
/codon_start=1
/product="Mei4p"
/protein_id="NP_010963.1"
/db_xref="GeneID:856768"
/db_xref="SGD:S000001954"
/translation="MSRGKLEDMEQKETSEVDWIICFALIQSRNPTLWKRALSRKKGD
VEDVGALKSEKNLKINPRENSKHIYKWVAPFENGFLNNKSLFAHLEPIYNFLCQNKYK
SFEDAVGLKELQSFSKDVSTADINNWFLPRYKILLKILSLKTKEIDFRGLSQVFQTLQ
ILLVSHYSHRIDSDSSFKRTLIDVHVFNFIAKFLFNRILLKKNQNDPKWLQNFYDQGD
GKHLCDKVDYKRLCSLHFTLIYSIINIQLIKIKTNQTFEPQILKYVSVLKLIEHILII
IESLIHVLIRFVSKHKLICINRKKAYCRVYLERELSLKKTYLKNFYSVISGVPEKELG
GLLKILKIVILSLLETFESIEWQHLKPFLEKFPAHEISLQKKRKYIQAALLITAERNL
IARFRLSRWFNETENI"
gene complement(<240032..>241501)
/gene="ACA1"
/locus_tag="YER045C"
/db_xref="GeneID:856769"
mRNA complement(<240032..>241501)
/gene="ACA1"
/locus_tag="YER045C"
/product="Aca1p"
/transcript_id="NM_001178936.3"
/db_xref="GeneID:856769"
CDS complement(240032..241501)
/gene="ACA1"
/locus_tag="YER045C"
/experiment="EXISTENCE:curator inference:GO:0005634
nucleus [PMID:10825197]"
/experiment="EXISTENCE:direct assay:GO:0001228 DNA-binding
transcription activator activity, RNA polymerase
II-specific [PMID:10825197]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:10825197]"
/experiment="EXISTENCE:direct assay:GO:0045944 positive
regulation of transcription by RNA polymerase II
[PMID:10825197]"
/note="ATF/CREB family basic leucine zipper (bZIP)
transcription factor; binds as a homodimer to the ATF/CREB
consensus sequence TGACGTCA; important for carbon source
utilization; target genes include GRE2 and COS8; ACA1 has
a paralog, CST6, that arose from the whole genome
duplication"
/codon_start=1
/product="Aca1p"
/protein_id="NP_010964.3"
/db_xref="GeneID:856769"
/db_xref="SGD:S000000847"
/translation="MDYKHNFATSPDSFLDGRQNPLLYTDFLSSNKELIYKQPSGPGL
VDSAYNFHHQNSLHDRSVQENLGPMFQPFGVDISHLPITNPPIFQSSLPAFDQPVYKR
RISISNGQISQLGEDLETVENLYNCQPPILSSKAQQNPNPQQVANPSAAIYPSFSSNE
LQNVPQPHEQATVIPEAAPQTGSKNIYAAMTPYDSNIKLNIPAVAATCDIPSATPSIP
SGDSTMNQAYINMQLRLQAQMQTKAWKNAQLNVHPCTPASNSSVSSSSSCQNINDHNI
ENQSVHSSISHGVNHHTVNNSCQNAELNISSSLPYESKCPDVNLTHANSKPQYKDATS
ALKNNINSEKDVHTAPFSSMHTTATFQIKQEARPQKIENNTAGLKDGAKAWKRARLLE
RNRIAASKCRQRKKMSQLQLQREFDQISKENTMMKKKIENYEKLVQKMKKISRLHMQE
CTINGGNNSYQSLQNKDSDVNGFLKMIEEMIRSSSLYDE"
gene <243180..>243611
/gene="SPO73"
/locus_tag="YER046W"
/db_xref="GeneID:856770"
mRNA <243180..>243611
/gene="SPO73"
/locus_tag="YER046W"
/product="Spo73p"
/transcript_id="NM_001178937.1"
/db_xref="GeneID:856770"
CDS 243180..243611
/gene="SPO73"
/locus_tag="YER046W"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:26605945|PMID:27303688]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:15590821]"
/experiment="EXISTENCE:mutant phenotype:GO:0030437
ascospore formation [PMID:11470404]"
/experiment="EXISTENCE:mutant phenotype:GO:0030476
ascospore wall assembly [PMID:15590821]"
/experiment="EXISTENCE:mutant phenotype:GO:0032120
ascospore-type prospore membrane formation
[PMID:26605945|PMID:27303688]"
/note="Meiosis-specific protein required for prospore
membrane morphogenesis; required for the proper shape of
the prospore membrane (PSM) and for spore wall formation;
functions cooperatively with SPO71 in PSM elongation;
physically interacts with Spo71p; genetically antagonistic
to SPO1, similar to SPO71; localizes to the PSM; required
for spore wall formation during sporulation; dispensable
for both nuclear divisions during meiosis; dysferlin
domain-only protein"
/codon_start=1
/product="Spo73p"
/protein_id="NP_010965.1"
/db_xref="GeneID:856770"
/db_xref="SGD:S000000848"
/translation="MGKNHFLKDFSALPEDVLIENERGITLLGYPLFSPKILLPHVDP
PQFQRLNTENGSLIALSKNTISNFIELYPIDLSTERTAGSSSSQMTKWFVLMDYKEKY
DIDDQGWCYSWNFNNSRWKSKNGLVRRRVWVRLPTTSHGLD"
gene complement(<243810..>246503)
/gene="SAP1"
/locus_tag="YER047C"
/db_xref="GeneID:856771"
mRNA complement(<243810..>246503)
/gene="SAP1"
/locus_tag="YER047C"
/product="putative AAA family ATPase SAP1"
/transcript_id="NM_001178938.1"
/db_xref="GeneID:856771"
CDS complement(243810..246503)
/gene="SAP1"
/locus_tag="YER047C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="Putative ATPase of the AAA family; interacts with
the Sin1p transcriptional repressor in the two-hybrid
system"
/codon_start=1
/product="putative AAA family ATPase SAP1"
/protein_id="NP_010966.1"
/db_xref="GeneID:856771"
/db_xref="SGD:S000000849"
/translation="MDSQRSHHILTRLTKIRRRPQQPLTDFTELYSRIANETIYYLNL
EEKKRYKEALQGWKALTTDVLFKQTLIEHNYPNTQSYTKDEVSLQNGIRELYHKSVMH
LKRVKKLVREEPAPRNDMPSSKTYTNHSSSFTRSTEPPPVFQMVPGRMMKTLRNRNAC
GYKTAYSNPSLSSYGNSTSIKRGEDAENIRVNFVPSKPLSNNASRQHKNPIEHNDPPL
KKETELYSDKYISEPILIDLTNDEDDHDVGILKGHNVFDEEESDGFEFDVSDYYDNFS
EVDVEEEEEEKEERRRIKTLEAIQQQMSDLSVTSSTSSNKSVSSSENVPGSCIQSLPT
TAPALPSLPPPPLLNVDRASSTGALKPHSLETSTTMDSSKIRNPQISKLMKNNHVPYL
KGTKSTPTLITKSTPTFITRSKSNTKPIIKSNASSPTSSLTVPNSVIQKPKTAAMAAK
RVLNSKKVASNPALNTTKKSHPILKSKTAKVPNSSSKKTSSHPSRPVSNSKPYSHGAS
QNKKPSKNQTTSMSKTNRKIPAQKKIGSPKIEDVGTEDATEHATSLNEQREEPEIDKK
VLREILEDEIIDSLQGVDRQAAKQIFAEIVVHGDEVHWDDIAGLESAKYSLKEAVVYP
FLRPDLFRGLREPVRGMLLFGPPGTGKTMLARAVATESHSTFFSISASSLTSKYLGES
EKLVRALFAIAKKLSPSIIFVDEIDSIMGSRNNENENESSRRIKNEFLVQWSSLSSAA
AGSNKSNTNNSDTNGDEDDTRVLVLAATNLPWSIDEAARRRFVRRQYIPLPEDQTRHV
QFKKLLSHQKHTLTESDFDELVKITEGYSGSDITSLAKDAAMGPLRDLGDKLLETERE
MIRPIGLVDFKNSLVYIKPSVSQDGLVKYEKWASQFGSSGS"
gene complement(<246982..>248157)
/gene="CAJ1"
/locus_tag="YER048C"
/db_xref="GeneID:856772"
mRNA complement(<246982..>248157)
/gene="CAJ1"
/locus_tag="YER048C"
/product="Caj1p"
/transcript_id="NM_001178939.3"
/db_xref="GeneID:856772"
CDS complement(246982..248157)
/gene="CAJ1"
/locus_tag="YER048C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:32777224]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:32777224]"
/experiment="EXISTENCE:direct assay:GO:1901981
phosphatidylinositol phosphate binding [PMID:39289920]"
/note="Nuclear type II J heat shock protein of the E. coli
dnaJ family; binds to numerous phosphatidylinositol
phosphates; overexpression stabilizes amino acid
permeases; binds non-native substrates for presentation to
Ssa3p, and may function during protein translocation,
assembly and disassembly; localizes to the cytosol and
plasma membrane, while a GFP fusion protein is nuclear;
contains an N-terminal J-domain, a leucine zipper-like
motif, and a putative C-terminal DNAJ-X domain"
/codon_start=1
/product="Caj1p"
/protein_id="NP_010967.3"
/db_xref="GeneID:856772"
/db_xref="SGD:S000000850"
/translation="MVKETEYYDILGIKPEATPTEIKKAYRRKAMETHPDKHPDDPDA
QAKFQAVGEAYQVLSDPGLRSKYDQFGKEDAVPQQGFEDASEYFTAIFGGDGFKDWIG
EFSLFKELNEATEMFGKEDEEGTAATETEKADESTDGGMVKHDTNKAESLKKDKLSKE
QREKLMEMEKKRREDMMKQVDELAEKLNEKISRYLIAVKSNNLEEFTRKLDQEIEDLK
LESFGLELLYLLARVYKTKANNFIMSKKTYGISKIFTGTRDNARSVKSAYNLLSTGLE
AQKAMEKMSEVNTDELDQYERAKFESTMAGKALGVMWAMSKFELERKLKDVCNKILND
KKVPSKERIAKAKAMLFIAHKFASARRSPEEAEEARVFEELILGEQEKEHKKHTVAR"
repeat_region complement(249109..249331)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006893"
repeat_region 249445..249752
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006913"
repeat_region complement(249930..250270)
/note="Ty3 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006904"
gene 250286..250357
/locus_tag="YNCE0014W"
/db_xref="GeneID:856773"
tRNA 250286..250357
/locus_tag="YNCE0014W"
/product="tRNA-Gln"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:15706032]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:15706032]"
/note="Glutamine tRNA (tRNA-Gln), predicted by tRNAscan-SE
analysis; thiolation of uridine at wobble position (34)
requires Ncs6p"
/db_xref="GeneID:856773"
/db_xref="SGD:S000006696"
gene <250718..>251002
/gene="ISD11"
/locus_tag="YER048W-A"
/db_xref="GeneID:856774"
mRNA <250718..>251002
/gene="ISD11"
/locus_tag="YER048W-A"
/product="Isd11p"
/transcript_id="NM_001184330.1"
/db_xref="GeneID:856774"
CDS 250718..251002
/gene="ISD11"
/locus_tag="YER048W-A"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:16341090|PMID:16341089]"
/experiment="EXISTENCE:direct assay:GO:0031071 cysteine
desulfurase activity [PMID:24045011]"
/experiment="EXISTENCE:direct assay:GO:0060090 molecular
adaptor activity [PMID:24045011]"
/experiment="EXISTENCE:mutant phenotype:GO:0016226
iron-sulfur cluster assembly
[PMID:16341089|PMID:16341090]"
/experiment="EXISTENCE:physical interaction:GO:0016226
iron-sulfur cluster assembly [PMID:16341090]"
/experiment="EXISTENCE:physical interaction:GO:1990221
L-cysteine desulfurase complex [PMID:24045011]"
/note="Cysteine desulfurase (Nfs1p) activator; essential
for the formation of the persulfide intermediate at the
desulfurase active site during pyridoxal
phosphate-dependent desulfuration of cysteine; required
for mitochondrial iron-sulfur cluster biosynthesis;
exclusive to eukaryotes, implicated as eukaryotic
supplement to the bacterium-derived Fe-S cluster (ISC)
assembly apparatus; involved in regulation of iron
metabolism; member of the LYR protein family"
/codon_start=1
/product="Isd11p"
/protein_id="NP_010968.1"
/db_xref="GeneID:856774"
/db_xref="SGD:S000007237"
/translation="MPGFTAPTRRQVLSLYKEFIKNANQFNNYNFREYFLSKTRTTFR
KNMNQQDPKVLMNLFKEAKNDLGVLKRQSVISQMYTFDRLVVEPLQGRKH"
gene <251728..>253662
/gene="TPA1"
/locus_tag="YER049W"
/db_xref="GeneID:856775"
mRNA <251728..>253662
/gene="TPA1"
/locus_tag="YER049W"
/product="oxidative DNA demethylase"
/transcript_id="NM_001178940.1"
/db_xref="GeneID:856775"
CDS 251728..253662
/gene="TPA1"
/locus_tag="YER049W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008143 poly(A)
binding [PMID:20040577]"
/experiment="EXISTENCE:direct assay:GO:0018188
peptidyl-proline di-hydroxylation
[PMID:24550462|PMID:31320474]"
/experiment="EXISTENCE:direct assay:GO:0031543
peptidyl-proline dioxygenase activity
[PMID:24550462|PMID:31320474]"
/experiment="EXISTENCE:mutant phenotype:GO:0000288
nuclear-transcribed mRNA catabolic process,
deadenylation-dependent decay [PMID:16809762]"
/experiment="EXISTENCE:mutant phenotype:GO:0006415
translational termination
[PMID:20630870|PMID:16809762|PMID:24550462]"
/experiment="EXISTENCE:mutant phenotype:GO:0006450
regulation of translational fidelity [PMID:24550462]"
/experiment="EXISTENCE:mutant phenotype:GO:0008198 ferrous
iron binding [PMID:25381260]"
/note="Prolyl hydroxylase; catalyzes oxygen-dependent
dihydroxylation of Rps23a/b, a 40S ribosomal decoding
center subunit; influences translational termination and
regulates translational accuracy; interacts with Sup45p
(eRF1), Sup35p (eRF3) and Pab1p; disputed role as a
oxidative dealkylase repairing DNA methyl-base lesions;
poly(rA)-binding protein affecting poly(A) tail length and
mRNA stability; Fe(II)/2-oxoglutarate-dependent
dioxygenase family member similar to human prolyl
4-hydroxylase OGFOD1"
/codon_start=1
/product="oxidative DNA demethylase"
/protein_id="NP_010969.1"
/db_xref="GeneID:856775"
/db_xref="SGD:S000000851"
/translation="MKRKTAEVKGEKERNSKQISLEEDKIKGMFNPKIWDKTFQDGLK
KEIEDSQPYNWGTIHELVNDDLLRAVRKEIETEIHFTKKETDIYRVNQSGDLANLSGL
DWDDLSRLPNLFKLRQILYSKQYRDFFGYVTKAGKLSGSKTDMSINTYTKGCHLLTHD
DVIGSRRISFILYLPDPDRKWKSHYGGGLRLFPSILPNVPHSDPSAKLVPQFNQIAFF
KVLPGFSFHDVEEVKVDKHRLSIQGWYHIPQVGEEGYIPGEEEAWVRNNTSTLAQIES
NVLEDFEFPKDERNILSFHEVKHFEKMLKGDAGAKTDNTPKESMTSVISDSVKLSEAE
FTYLSQYISPEHLSSKGIEKLQKQFVENSSLQIESFLNDDKSELLKKVIKQKELEQEC
PYHSKDVKAPWKTAIPPHKARYLYIDGKEYRNFQTEADILEALNNNDLPNFQFTKDAI
KIISDASGNSRENNFDAELALIDLAVFHKSTIFKKYLALLTSLCPVSEQILIRRFRPG
MDFTLATKCRFNELLKSNPDIIDAVLEGTLCLTPSAGWESGELGGYELYMMDDDEDNK
QYLKEDVEDASVYRADDSGDSVLINDPPAWNTFNLVLRDESVLEFVKYVSWSAKSSRW
DVKMKWDVKSCDEDGQEDEA"
gene complement(<253971..>254387)
/gene="RSM18"
/locus_tag="YER050C"
/gene_synonym="bS18m"
/db_xref="GeneID:856776"
mRNA complement(<253971..>254387)
/gene="RSM18"
/locus_tag="YER050C"
/gene_synonym="bS18m"
/product="mitochondrial 37S ribosomal protein bS18m RSM18"
/transcript_id="NM_001178941.3"
/db_xref="GeneID:856776"
CDS complement(253971..254387)
/gene="RSM18"
/locus_tag="YER050C"
/gene_synonym="bS18m"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:11278769]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:11278769]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005763
mitochondrial small ribosomal subunit [PMID:11278769]"
/note="Mitochondrial ribosomal protein of the small
subunit; has similarity to E. coli S18 ribosomal protein"
/codon_start=1
/product="mitochondrial 37S ribosomal protein bS18m RSM18"
/protein_id="NP_010970.4"
/db_xref="GeneID:856776"
/db_xref="SGD:S000000852"
/translation="MQPIIKGAVSSTFKRALYNFGIKEKKSVNIEMGRTQQTKKIDQS
LSKKLPKGTIYDPFDFSMGRIHLDRKYQANKNSNRNDIMKSGANPLEFYARPRILSRY
VTSTGRIQHRDITGLSAKNQRRLSKAIRRCQAIGLM"
gene <254656..>256134
/gene="JHD1"
/locus_tag="YER051W"
/gene_synonym="KDM2"
/db_xref="GeneID:856777"
mRNA <254656..>256134
/gene="JHD1"
/locus_tag="YER051W"
/gene_synonym="KDM2"
/product="[Histone H3]-lysine-36 demethylase"
/transcript_id="NM_001178942.1"
/db_xref="GeneID:856777"
CDS 254656..256134
/gene="JHD1"
/locus_tag="YER051W"
/gene_synonym="KDM2"
/EC_number="1.14.11.27"
/experiment="EXISTENCE:direct assay:GO:0051864 histone
H3K36 demethylase activity [PMID:16362057]"
/experiment="EXISTENCE:direct assay:GO:0140002 histone
H3K4me3 reader activity [PMID:17142463]"
/experiment="EXISTENCE:genetic interaction:GO:0032968
positive regulation of transcription elongation by RNA
polymerase II [PMID:17525156]"
/experiment="EXISTENCE:mutant phenotype:GO:0051864 histone
H3K36 demethylase activity [PMID:17369256]"
/note="JmjC domain family histone demethylase specific for
H3-K36; similar to proteins found in human, mouse,
drosophila, X. laevis, C. elegans, and S. pombe"
/codon_start=1
/product="[Histone H3]-lysine-36 demethylase"
/protein_id="NP_010971.1"
/db_xref="GeneID:856777"
/db_xref="SGD:S000000853"
/translation="MQDPNICQHCQLKDNPGALIWVKCDSCPQWVHVKCVPLKRIHYS
NLTSSEVLSYPNSAKQIKSYRCPNHKEGEYLTAYALITQKGKRQRNKENPEDSHINKR
YNFRKKKLLDYIALNEGESKRDKMNHPHKESFMKSFEKWKNGSNIINAADFAEKFDNI
DVPYKIIDPLNSGVYVPNVGTDNGCLTVNYITEMIGEDYHVDVMDVQSQMNENWNLGS
WNEYFTNTEPDRRDRIRNVISLEVSNIEGLELERPTAVRQNDLVDKIWSFNGHLEKVN
GEKAEENDPKPKVTKYILMSVKDAYTDFHLDFAGTSVYYNVISGQKKFLLFPPTQSNI
DKYIEWSLKEDQNSVFLGDILEDGIAMELDAGDLFMIPAGYIHAVYTPVDSLVFGGNF
LTIRDLETHLKIVEIEKLTKVPRRFTFPKFDQVMGKLCEYLALDKNKITSDVSDGDLL
SRTTNCAIQSLHAYVIKPEVKYKPLNFTSKKHLAKALADLIS"
gene complement(<256375..>257958)
/gene="HOM3"
/locus_tag="YER052C"
/gene_synonym="BOR1; SIL4; THR3"
/db_xref="GeneID:856778"
mRNA complement(<256375..>257958)
/gene="HOM3"
/locus_tag="YER052C"
/gene_synonym="BOR1; SIL4; THR3"
/product="aspartate kinase"
/transcript_id="NM_001178943.1"
/db_xref="GeneID:856778"
CDS complement(256375..257958)
/gene="HOM3"
/locus_tag="YER052C"
/gene_synonym="BOR1; SIL4; THR3"
/EC_number="2.7.2.4"
/experiment="EXISTENCE:direct assay:GO:0004072 aspartate
kinase activity [PMID:18626862]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0009088 L-threonine
biosynthetic process [PMID:18626862]"
/experiment="EXISTENCE:mutant phenotype:GO:0004072
aspartate kinase activity [PMID:18626862]"
/experiment="EXISTENCE:mutant phenotype:GO:0009086
methionine biosynthetic process [PMID:4380684]"
/experiment="EXISTENCE:mutant phenotype:GO:0009088
L-threonine biosynthetic process
[PMID:18626862|PMID:4380684]"
/experiment="EXISTENCE:mutant phenotype:GO:0009090
L-homoserine biosynthetic process [PMID:4380684]"
/note="Aspartate kinase (L-aspartate 4-P-transferase);
cytoplasmic enzyme that catalyzes the first step in the
common pathway for methionine and threonine biosynthesis;
subject to allosteric feedback inhibition by threonine;
expression regulated by Gcn4p and the general control of
amino acid synthesis"
/codon_start=1
/product="aspartate kinase"
/protein_id="NP_010972.1"
/db_xref="GeneID:856778"
/db_xref="SGD:S000000854"
/translation="MPMDFQPTSSHSNWVVQKFGGTSVGKFPVQIVDDIVKHYSKPDG
PNNNVAVVCSARSSYTKAEGTTSRLLKCCDLASQESEFQDIIEVIRQDHIDNADRFIL
NPALQAKLVDDTNKELELVKKYLNASKVLGEVSSRTVDLVMSCGEKLSCLFMTALCND
RGCKAKYVDLSHIVPSDFSASALDNSFYTFLVQALKEKLAPFVSAKERIVPVFTGFFG
LVPTGLLNGVGRGYTDLCAALIAVAVNADELQVWKEVDGIFTADPRKVPEARLLDSVT
PEEASELTYYGSEVIHPFTMEQVIRAKIPIRIKNVQNPLGNGTIIYPDNVAKKGESTP
PHPPENLSSSFYEKRKRGATAITTKNDIFVINIHSNKKTLSHGFLAQIFTILDKYKLV
VDLISTSEVHVSMALPIPDADSLKSLRQAEEKLRILGSVDITKKLSIVSLVGKHMKQY
IGIAGTMFTTLAEEGINIEMISQGANEINISCVINESDSIKALQCIHAKLLSERTNTS
NQFEHAIDERLEQLKRLGI"
rep_origin 257958..258737
/note="ARS513; Autonomously Replicating Sequence"
/db_xref="SGD:S000077385"
gene complement(<258737..>259639)
/gene="PIC2"
/locus_tag="YER053C"
/db_xref="GeneID:856779"
mRNA complement(<258737..>259639)
/gene="PIC2"
/locus_tag="YER053C"
/product="Cu/Pi carrier"
/transcript_id="NM_001178944.3"
/db_xref="GeneID:856779"
CDS complement(258737..259639)
/gene="PIC2"
/locus_tag="YER053C"
/experiment="EXISTENCE:direct assay:GO:0005315 phosphate
transmembrane transporter activity [PMID:14756774]"
/experiment="EXISTENCE:direct assay:GO:0005375 copper ion
transmembrane transporter activity [PMID:23846699]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:14576278|PMID:16823961|PMID:24769239|PMID:14756774]"
/experiment="EXISTENCE:direct assay:GO:0035434 copper ion
transmembrane transport [PMID:23846699]"
/experiment="EXISTENCE:direct assay:GO:0035435 phosphate
ion transmembrane transport [PMID:14756774]"
/experiment="EXISTENCE:mutant phenotype:GO:0006878
intracellular copper ion homeostasis [PMID:23846699]"
/note="Mitochondrial copper and phosphate carrier; imports
copper and inorganic phosphate into mitochondria;
functionally redundant with Mir1p but less abundant than
Mir1p under normal conditions; expression is induced at
high temperature"
/codon_start=1
/product="Cu/Pi carrier"
/protein_id="NP_010973.3"
/db_xref="GeneID:856779"
/db_xref="SGD:S000000855"
/translation="MESNKQPRKIQLYTKEFYATCTLGGIIACGPTHSSITPLDLVKC
RLQVNPKLYTSNLQGFRKIIANEGWKKVYTGFGATFVGYSLQGAGKYGGYEYFKHLYS
SWLSPGVTVYLMASATAEFLADIMLCPFEAIKVKQQTTMPPFCNNVVDGWKKMYAESG
GMKAFYKGIVPLWCRQIPYTMCKFTSFEKIVQKIYSVLPKKKEEMNALQQISVSFVGG
YLAGILCAAVSHPADVMVSKINSERKANESMSVASKRIYQKIGFTGLWNGLMVRIVMI
GTLTSFQWLIYDSFKAYVGLPTTG"
gene complement(<260933..>261046)
/locus_tag="YER053C-A"
/db_xref="GeneID:856780"
mRNA complement(<260933..>261046)
/locus_tag="YER053C-A"
/product="uncharacterized protein"
/transcript_id="NM_001184444.1"
/db_xref="GeneID:856780"
CDS complement(260933..261046)
/locus_tag="YER053C-A"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to the endoplasmic
reticulum; protein abundance increases in response to DNA
replication stress"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_061492.1"
/db_xref="GeneID:856780"
/db_xref="SGD:S000007523"
/translation="MQDLEIFLSIFAFIFVFYFGAHRTVMNRNKSDVPYLQ"
gene complement(<262052..>263698)
/gene="GIP2"
/locus_tag="YER054C"
/db_xref="GeneID:856781"
mRNA complement(<262052..>263698)
/gene="GIP2"
/locus_tag="YER054C"
/product="Gip2p"
/transcript_id="NM_001178945.1"
/db_xref="GeneID:856781"
CDS complement(262052..263698)
/gene="GIP2"
/locus_tag="YER054C"
/experiment="EXISTENCE:direct assay:GO:0019888 protein
phosphatase regulator activity [PMID:8754819]"
/experiment="EXISTENCE:mutant phenotype:GO:0005977
glycogen metabolic process [PMID:11973298]"
/experiment="EXISTENCE:physical interaction:GO:0000164
protein phosphatase type 1 complex [PMID:8754819]"
/note="Putative regulatory subunit of protein phosphatase
Glc7p; involved in glycogen metabolism; contains a
conserved motif (GVNK motif) that is also found in Gac1p,
Pig1p, and Pig2p; GIP2 has a paralog, PIG2, that arose
from the whole genome duplication"
/codon_start=1
/product="Gip2p"
/protein_id="NP_010974.1"
/db_xref="GeneID:856781"
/db_xref="SGD:S000000856"
/translation="MYIKAEQKPQQFERKNEKLDRNKNQQLPDLETDFKGYRVNSDLY
NKERDGSTEETLNSLKFLHKPQRVTQMRANRFPEEEVQRNTDLNKRIFSAGNDENVDN
ESGWSKIAAAKNHTSVESLNGSTRPPFKIELPPLSPKSTVPKSFQAEYPEAKSPGNDM
NFEYDEEILIPFAPPVYKKSGELLKSSLKRRSKSLPTTPGIRSGNGVQARDGSPMLIR
SKSVHFDQAAPVKYFAEDESPINVNKTEQHDNCLSFKHKPVNLMVDPEEETKMLSSGL
ETTSIDDDLTTVAPKGFAHPAKISNPNNGKGTNNTKLRKSKRFQNLLKNRTDMPPSKS
NKKFVNGGGAHEISDRNSKNYHVVGLYSKNFPILSNKNPKSLKLNIFINLSQNKKVFL
QELSLYIHRDNNYFSNSSSFYNIPNSHNGNDCNGVAKGYNAGCTRLIAGRILVKNIFY
DKRVVVRYTWDSWRTTHEVECVYISDGDGILPGTNMDIFHFIIDDVSKVDPRGKLEFC
IHYSTRNDYEREEYWDNNNGNNYKVDVVMDGFNDPFAAAA"
gene complement(<264892..>265785)
/gene="HIS1"
/locus_tag="YER055C"
/db_xref="GeneID:856782"
mRNA complement(<264892..>265785)
/gene="HIS1"
/locus_tag="YER055C"
/product="ATP phosphoribosyltransferase"
/transcript_id="NM_001178946.3"
/db_xref="GeneID:856782"
CDS complement(264892..265785)
/gene="HIS1"
/locus_tag="YER055C"
/EC_number="2.4.2.17"
/experiment="EXISTENCE:mutant phenotype:GO:0000105
L-histidine biosynthetic process [PMID:14190241]"
/experiment="EXISTENCE:mutant phenotype:GO:0003879 ATP
phosphoribosyltransferase activity [PMID:14190241]"
/note="ATP phosphoribosyltransferase; a hexameric enzyme,
catalyzes the first step in histidine biosynthesis;
mutations cause histidine auxotrophy and sensitivity to
Cu, Co, and Ni salts; transcription is regulated by
general amino acid control"
/codon_start=1
/product="ATP phosphoribosyltransferase"
/protein_id="NP_010975.3"
/db_xref="GeneID:856782"
/db_xref="SGD:S000000857"
/translation="MDLVNHLTDRLLFAIPKKGRLYSKSVSILNGADITFHRSQRLDI
ALSTSLPVALVFLPAADIPTFVGEGKCDLGITGVDQVRESNVDVDLAIDLQFGNCKLQ
VQVPVNGEYKKPEQLIGKTIVTSFVKLAEKYFADLEGTTVEKMTTRIKFVSGSVEASC
ALGIGDAIVDLVESGETMRAAGLVDIATVLSTSAYLIESKNPKSDKSLIATIKSRIEG
VMTAQRFVSCIYNAPEDKLPELLKVTPGRRAPTISKIDDEGWVAVSSMIERKTKGVVL
DELKRLGASDIMVFEISNCRV"
gene complement(<266512..>268113)
/gene="FCY2"
/locus_tag="YER056C"
/gene_synonym="BRA7"
/db_xref="GeneID:856783"
mRNA complement(<266512..>268113)
/gene="FCY2"
/locus_tag="YER056C"
/gene_synonym="BRA7"
/product="purine-cytosine permease"
/transcript_id="NM_001178947.1"
/db_xref="GeneID:856783"
CDS complement(266512..268113)
/gene="FCY2"
/locus_tag="YER056C"
/gene_synonym="BRA7"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:22842922|PMID:8267570]"
/experiment="EXISTENCE:direct assay:GO:0015205 nucleobase
transmembrane transporter activity [PMID:9092500]"
/experiment="EXISTENCE:direct assay:GO:0015212 cytidine
transmembrane transporter activity [PMID:10501935]"
/experiment="EXISTENCE:direct assay:GO:0015856 cytosine
transport [PMID:9092500]"
/experiment="EXISTENCE:direct assay:GO:0015861 cytidine
transport [PMID:10501935]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0072530
purine-containing compound transmembrane transport
[PMID:9092500]"
/experiment="EXISTENCE:mutant phenotype:GO:0015212
cytidine transmembrane transporter activity
[PMID:10501935]"
/experiment="EXISTENCE:mutant phenotype:GO:0015861
cytidine transport [PMID:10501935]"
/note="Purine-cytosine permease; mediates purine (adenine,
guanine, and hypoxanthine) and cytosine accumulation;
relative distribution to the vacuole increases upon DNA
replication stress"
/codon_start=1
/product="purine-cytosine permease"
/protein_id="NP_010976.1"
/db_xref="GeneID:856783"
/db_xref="SGD:S000000858"
/translation="MLEEGNNVYEIQDLEKRSPVIGSSLENEKKVAASETFTATSEDD
QQYIVESSEATKLSWFHKFFASLNAETKGVEPVTEDEKTDDSILNAASMWFSANMVIA
SYALGALGPMVFGLNFGQSVLVIIFFNIMGLIFVAFFSVFGAELGLRQMILSRYLVGN
VTARIFSLINVIACVGWGIVNTSVSAQLLNMVNEGSGHVCPIWAGCLIIIGGTVLVTF
FGYSVIHAYEKWSWVPNFAVFLVIIAQLSRSGKFKGGEWVGGATTAGSVLSFGSSIFG
FAAGWTTYAADYTVYMPKSTNKYKIFFSLVAGLAFPLFFTMILGAASAMAALNDPTWK
AYYDKNAMGGVIYAILVPNSLNGFGQFCCVLLALSTIANNIPNMYTVALSAQALWAPL
AKIPRVVWTMAGNAATLGISIPATYYFDGFMENFMDSIGYYLAIYIAISCSEHFFYRR
SFSAYNIDDWDNWEHLPIGIAGTAALIVGAFGVALGMCQTYWVGEIGRLIGKYGGDIG
FELGASWAFIIYNILRPLELKYFGR"
gene complement(<269423..>270185)
/gene="RPL34A"
/locus_tag="YER056C-A"
/db_xref="GeneID:856784"
mRNA complement(join(<269423..269751,270149..>270185))
/gene="RPL34A"
/locus_tag="YER056C-A"
/product="60S ribosomal protein eL34 RPL34A"
/transcript_id="NM_001180036.1"
/db_xref="GeneID:856784"
CDS complement(join(269423..269751,270149..270185))
/gene="RPL34A"
/locus_tag="YER056C-A"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:23874617]"
/experiment="EXISTENCE:curator inference:GO:0003735
structural constituent of ribosome [PMID:23874617]"
/experiment="EXISTENCE:direct assay:GO:0030687
preribosome, large subunit precursor [PMID:23874617]"
/experiment="EXISTENCE:direct assay:GO:0042254 ribosome
biogenesis [PMID:23874617]"
/note="Ribosomal 60S subunit protein L34A; homologous to
mammalian ribosomal protein L34, no bacterial homolog;
RPL34A has a paralog, RPL34B, that arose from the whole
genome duplication"
/codon_start=1
/product="60S ribosomal protein eL34 RPL34A"
/protein_id="NP_010977.2"
/db_xref="GeneID:856784"
/db_xref="SGD:S000002135"
/translation="MAQRVTFRRRNPYNTRSNKIKVVKTPGGILRAQHVKKLATRPKC
GDCGSALQGISTLRPRQYATVSKTHKTVSRAYGGSRCANCVKERIIRAFLIEEQKIVK
KVVKEQTEAAKKSEKKAKK"
gene complement(<270737..>271126)
/gene="HMF1"
/locus_tag="YER057C"
/gene_synonym="HIG1"
/db_xref="GeneID:856785"
mRNA complement(<270737..>271126)
/gene="HMF1"
/locus_tag="YER057C"
/gene_synonym="HIG1"
/product="putative isoleucine biosynthesis protein HMF1"
/transcript_id="NM_001178948.3"
/db_xref="GeneID:856785"
CDS complement(270737..271126)
/gene="HMF1"
/locus_tag="YER057C"
/gene_synonym="HIG1"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11442631]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11442631]"
/experiment="EXISTENCE:direct assay:GO:0005758
mitochondrial intermembrane space [PMID:22984289]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:11003673]"
/note="Member of the p14.5 protein family; functionally
complements Mmf1p function when targeted to mitochondria;
heat shock inducible; high-dosage growth inhibitor; forms
a homotrimer in vitro; HMF1 has a paralog, MMF1, that
arose from the whole genome duplication"
/codon_start=1
/product="putative isoleucine biosynthesis protein HMF1"
/protein_id="NP_010978.3"
/db_xref="GeneID:856785"
/db_xref="SGD:S000000859"
/translation="MVTTLTPVICESAPAAAASYSHAMKVNNLIFLSGQIPVTPDNKL
VEGSIADKAEQVIQNIKNVLEASNSSLDRVVKVNIFLADINHFAEFNSVYAKYFNTHK
PARSCVAVAALPLGVDMEMEAIAAERD"
gene <271768..>272091
/gene="PET117"
/locus_tag="YER058W"
/db_xref="GeneID:856786"
mRNA <271768..>272091
/gene="PET117"
/locus_tag="YER058W"
/product="Pet117p"
/transcript_id="NM_001178949.1"
/db_xref="GeneID:856786"
CDS 271768..272091
/gene="PET117"
/locus_tag="YER058W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:mutant phenotype:GO:0033617
mitochondrial respiratory chain complex IV assembly
[PMID:8381337|PMID:27998984]"
/note="Assembly factor that couples heme a synthesis to
complex IV assembly; regulates the oligomerization state
of the Cox15p heme a synthase, and couples this to the
assembly of the cytochrome C oxidase complex (complex IV)"
/codon_start=1
/product="Pet117p"
/protein_id="NP_010979.1"
/db_xref="GeneID:856786"
/db_xref="SGD:S000000860"
/translation="MSRASKITFAASCLITAATVVGVHYVQEMERETLHQGPIKDAKR
VEEKRLRKTNGVASLDPTKERKRYFNMSEHEEQKELRKKYETMQPLSGEVVTKDGEVV
KESKK"
gene <272624..>273886
/gene="PCL6"
/locus_tag="YER059W"
/db_xref="GeneID:856787"
mRNA <272624..>273886
/gene="PCL6"
/locus_tag="YER059W"
/product="Pcl6p"
/transcript_id="NM_001178950.1"
/db_xref="GeneID:856787"
CDS 272624..273886
/gene="PCL6"
/locus_tag="YER059W"
/experiment="EXISTENCE:genetic interaction:GO:0005979
regulation of glycogen biosynthetic process
[PMID:11602261]"
/experiment="EXISTENCE:genetic interaction:GO:0005981
regulation of glycogen catabolic process [PMID:11602261]"
/note="Pho85p cyclin of the Pho80p subfamily; forms the
major Glc8p kinase together with Pcl7p and Pho85p;
involved in the control of glycogen storage by Pho85p;
stabilized by Elongin C binding; PCL6 has a paralog, PCL7,
that arose from the whole genome duplication"
/codon_start=1
/product="Pcl6p"
/protein_id="NP_010980.1"
/db_xref="GeneID:856787"
/db_xref="SGD:S000000861"
/translation="MSIKGDSPSSTNASSSPKSTYSIQSDDKANLGSGNVDIRTDNSQ
QDSNNRRDIVVVTRVASEETLESQSSTSSMGIRPESSFNYEDASNQARVEMNNRVHGS
NMNTINKYYPVRFPKNNERQLSDTNNLNEKVQGTHTVQSSTQEDKILDGDTSNSQVTP
SLNIAEFPTDKLLKMLTALLTKIIKSNDRTAATNPSLTQEIENGRCLALSDNEKKYLS
PVLGFRGKHVPQIGLDQYFQRIQKYCPTTNDVFLSLLVYFDRISKRCNSVTTTPKTNT
AKHESPSNESSLDKANRGADKMSACNSNENNENDDSDDENTGVQRDSRAHPQMFVMDS
HNIHRLIIAGITVSTKFLSDFFYSNSRYSRVGGISLQELNHLELQFLVLCDFELLISV
NELQRYADLLYRFWNNAKAQSQALVTGM"
gene <274567..>276153
/gene="FCY21"
/locus_tag="YER060W"
/db_xref="GeneID:856788"
mRNA <274567..>276153
/gene="FCY21"
/locus_tag="YER060W"
/product="purine-cytosine permease"
/transcript_id="NM_001178951.3"
/db_xref="GeneID:856788"
CDS 274567..276153
/gene="FCY21"
/locus_tag="YER060W"
/experiment="EXISTENCE:mutant phenotype:GO:0015205
nucleobase transmembrane transporter activity
[PMID:16845689]"
/experiment="EXISTENCE:mutant phenotype:GO:0015856
cytosine transport [PMID:16845689]"
/note="Putative purine-cytosine permease; very similar to
Fcy2p but cannot substitute for its function"
/codon_start=1
/product="purine-cytosine permease"
/protein_id="NP_010981.3"
/db_xref="GeneID:856788"
/db_xref="SGD:S000000862"
/translation="MPQTHEMSLNGTQYLKYELKDLESRAHDAKTPSTNEFYDDVESH
GTEELVEAKLSFLNRIAAGLSAETKGIEPITEDEKTDDSILNAASMWFSANMVLPAYA
IGALGPMVFDLNFGQSVFVIIFFNLLGLVSVAFFSVFGAELGLRQMILSRYLVGNIAA
RIFSFINFIACIGWGIVNTVASSQVLNMVNPGHQCPLWAGCIVIIGATVIVTFFGYGV
IHAYEKWAWVPNFAVFLVIIARLARSKKFVLGEWTSGPTTAGNVLSFGSTVYGFAAGW
TTYAADYTVYMPRKTNKYKIFFSLVVGLATPLYFTMILGAAVAMAAIGDPAWKTYYDE
NSIGGLTFAVLVPNSVHGFGQFCCVLLSLSTIANNVPNMYTIALSVQATWEPLAKVPR
VIWTLLGNAAALGIAIPACYYFSTFMNYFMDSIGYYLAIYIAIACSEHFIYRRSFSAY
NVDDWDSWERLPIGIAGTAALIVGAFGVALGMCQTYWVGEISRLIGDYGGDIGFELGL
SWAFIVYNIARPFELKYFGR"
rep_origin 276130..276528
/note="ARS513.5; Very weak autonomously replicating
sequence"
/db_xref="SGD:S000178086"
gene <276572..>278164
/gene="FCY22"
/locus_tag="YER060W-A"
/db_xref="GeneID:856789"
mRNA <276572..>278164
/gene="FCY22"
/locus_tag="YER060W-A"
/product="purine-cytosine permease"
/transcript_id="NM_001180858.1"
/db_xref="GeneID:856789"
CDS 276572..278164
/gene="FCY22"
/locus_tag="YER060W-A"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0015205
nucleobase transmembrane transporter activity
[PMID:16845689]"
/experiment="EXISTENCE:mutant phenotype:GO:0015856
cytosine transport [PMID:16845689]"
/note="Putative purine-cytosine permease; very similar to
Fcy2p but cannot substitute for its function"
/codon_start=1
/product="purine-cytosine permease"
/protein_id="NP_010982.1"
/db_xref="GeneID:856789"
/db_xref="SGD:S000002958"
/translation="MPEKLAMSMVDIKDAGSELRDLESGALDTKSSAADVYYEGVELH
RTNEFIDNKPSFFNRIAAALNAETKGIEPVTEDEKNDDSILNAATIWFSANMVIVAYS
VGALGPLVFGLNFGQSVLVIIFFNILGLIPVALFSLFGVELGLRQMILSRYLAGNITA
RFFSLVNVIACVGWCVLNISVSAQLLNMVNEGSGHNCPIWAGCLIIAGGTVLVTFFGY
SVVHAYEKWSWVPNFAAFLVIIAQLSRSGKFKGGEWVGGATTAGGVLSFGSSVFGSAA
GWATYAADYTVYMPKTTSKYKIFFSVVAGLAFPLFFTMILGAACGMAALNDPTWKSYY
DKNAMGGVIYAILVPNSLNGFGQFCCVLLALSTVANNVPGMYTVALSAQALWAPLAKI
PRVVWTMAGNAATLGISIPATYYFDGFMENFMDSIGYYLAIYIAIACSEHFIYRRSFS
AYNIDDWDNWEHLPIGIAGTAALIAGAFGVALGMCQTYWVGEISRLIGEYGGDIGFEL
GGSWAFIIYNIVRPLELKYFGR"
rep_origin 278076..278357
/note="ARS513.7; Weak autonomously replicating sequence"
/db_xref="SGD:S000178087"
gene complement(<278298..>279626)
/gene="CEM1"
/locus_tag="YER061C"
/db_xref="GeneID:856790"
mRNA complement(<278298..>279626)
/gene="CEM1"
/locus_tag="YER061C"
/product="fatty acid synthase CEM1"
/transcript_id="NM_001178952.2"
/db_xref="GeneID:856790"
CDS complement(278298..279626)
/gene="CEM1"
/locus_tag="YER061C"
/EC_number="2.3.1.41"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095|PMID:24769239|PMID:16823961]"
/note="Mitochondrial beta-keto-acyl synthase; possible
role in fatty acid synthesis; required for mitochondrial
respiration; human homolog OXSM can complement yeast cem1
null mutant"
/codon_start=1
/product="fatty acid synthase CEM1"
/protein_id="NP_010983.2"
/db_xref="GeneID:856790"
/db_xref="SGD:S000000863"
/translation="MSRRVVITGLGCVTPLGRSLSESWGNLLSSKNGLTPITSLPNYN
EDYKLREKSIPSTITVGKIPENFQNENSAINKLLFTSQDERRTSSFIKLALRTTYEAL
HNAGLLNPNDITINTSLCNLDHFGCLIGSGIGSIQDIYQTSLQFHNDNKRINPYFVPK
ILTNMAAGNVSIKFNLRGLSHSVSTACATGNNSIGDAFNFIRLGMQDICVAGASETSL
HPLSLAGFIRAKSITTNGISRPFDTQRSGFVLGEGCGMIVMESLEHAQKRNANIISEL
VGYGLSSDACHITSPPADGNGAKRAIEMALKMARLEPTDVDYVNAHATSTLLGDKAEC
LAVASALLPGRSKSKPLYISSNKGAIGHLLGAAGAVESIFTICSLKDDKMPHTLNLDN
VLTLENNEADKLHFIRDKPIVGANPKYALCNSFGFGGVNTSLLFKKWEGS"
gene complement(<279930..>280682)
/gene="GPP2"
/locus_tag="YER062C"
/gene_synonym="HOR2"
/db_xref="GeneID:856791"
mRNA complement(<279930..>280682)
/gene="GPP2"
/locus_tag="YER062C"
/gene_synonym="HOR2"
/product="glycerol-1-phosphatase GPP2"
/transcript_id="NM_001178953.3"
/db_xref="GeneID:856791"
CDS complement(279930..280682)
/gene="GPP2"
/locus_tag="YER062C"
/gene_synonym="HOR2"
/EC_number="3.1.3.21"
/experiment="EXISTENCE:direct assay:GO:0000121 sn-glycerol
1-phosphatase activity [PMID:8662716]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005975
carbohydrate metabolic process [PMID:8662716]"
/experiment="EXISTENCE:direct assay:GO:0006970 response to
osmotic stress [PMID:8662716]"
/experiment="EXISTENCE:mutant phenotype:GO:0006114
glycerol biosynthetic process [PMID:11058591]"
/note="DL-glycerol-3-phosphate phosphatase involved in
glycerol biosynthesis; also known as
glycerol-1-phosphatase; induced in response to
hyperosmotic or oxidative stress, and during diauxic
shift; GPP2 has a paralog, GPP1, that arose from the whole
genome duplication"
/codon_start=1
/product="glycerol-1-phosphatase GPP2"
/protein_id="NP_010984.3"
/db_xref="GeneID:856791"
/db_xref="SGD:S000000864"
/translation="MGLTTKPLSLKVNAALFDVDGTIIISQPAIAAFWRDFGKDKPYF
DAEHVIQVSHGWRTFDAIAKFAPDFANEEYVNKLEAEIPVKYGEKSIEVPGAVKLCNA
LNALPKEKWAVATSGTRDMAQKWFEHLGIRRPKYFITANDVKQGKPHPEPYLKGRNGL
GYPINEQDPSKSKVVVFEDAPAGIAAGKAAGCKIIGIATTFDLDFLKEKGCDIIVKNH
ESIRVGGYNAETDEVEFIFDDYLYAKDDLLKW"
gene <281710..>282366
/gene="THO1"
/locus_tag="YER063W"
/db_xref="GeneID:856792"
mRNA <281710..>282366
/gene="THO1"
/locus_tag="YER063W"
/product="Tho1p"
/transcript_id="NM_001178954.1"
/db_xref="GeneID:856792"
CDS 281710..282366
/gene="THO1"
/locus_tag="YER063W"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:16738307]"
/experiment="EXISTENCE:direct assay:GO:0003690
double-stranded DNA binding [PMID:16738307]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:16738307]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0006368
transcription elongation by RNA polymerase II
[PMID:9707445]"
/experiment="EXISTENCE:genetic interaction:GO:0016973
poly(A)+ mRNA export from nucleus [PMID:16738307]"
/experiment="EXISTENCE:mutant phenotype:GO:0022618
protein-RNA complex assembly [PMID:16738307]"
/note="Conserved nuclear RNA-binding protein; specifically
binds to transcribed chromatin in a THO- and RNA-dependent
manner, genetically interacts with shuttling hnRNP NAB2;
overproduction suppresses transcriptional defect caused by
hpr1 mutation"
/codon_start=1
/product="Tho1p"
/protein_id="NP_010985.1"
/db_xref="GeneID:856792"
/db_xref="SGD:S000000865"
/translation="MADYSSLTVVQLKDLLTKRNLSVGGLKNELVQRLIKDDEESKGE
SEVSPQEQNQEQGSEPAAIEEPASQNITEKKEVSSEPKETNEPKEENKDVQKPSDGPS
ATASENEQAAASTAAPALSPEEIKAKALDLLNKKLHRANKFGQDQADIDSLQRQINRV
EKFGVDLNSKLAEELGLVSRKNEPESGNNGKFKNRNKNANNRSRVSKNRRGNRSGYRR
"
gene complement(<282705..>284222)
/gene="VHR2"
/locus_tag="YER064C"
/db_xref="GeneID:856793"
mRNA complement(<282705..>284222)
/gene="VHR2"
/locus_tag="YER064C"
/product="Vhr2p"
/transcript_id="NM_001178955.1"
/db_xref="GeneID:856793"
CDS complement(282705..284222)
/gene="VHR2"
/locus_tag="YER064C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:mutant phenotype:GO:0006355
regulation of DNA-templated transcription [PMID:11342098]"
/note="Non-essential nuclear protein; null mutation has
global effects on transcription; VHR2 has a paralog, VHR1,
that arose from the whole genome duplication; relative
distribution to the nucleus increases upon DNA replication
stress"
/codon_start=1
/product="Vhr2p"
/protein_id="NP_010986.1"
/db_xref="GeneID:856793"
/db_xref="SGD:S000000866"
/translation="MIDDTENSKIHLEGSHKTGKYTGYGTTHKIRAQLNFNDEKKWKK
FSSRRLELIDSFGLSQHKASEQDDNIRQIATILRSEFEYPDTFSAEFEKLVTAAVQSV
RRNRKRSKKKLLDSKKKIARGKVQKIPLSPPSSSNMGSCSASNASSSDEEASVKEEPA
EHALPSLNTITSQKLLPYPNGRTLPPVPTQVRSLLKKNASLLRDPSAPYAHGGDEKLQ
KFDIEDQPLESEQEYDFIAKSIIVEIVNNAIPLPEQIQRDKFIRPNLTKKKGCQSKVV
ISNNLRKLILSKIHNSRTCLEMSKDERNLDSFANLETLGKNSLMASISLVVENSFSHL
PSSTKQYLTERLSSIEFLTILSQRLFMPATRQLFADLSQEKIQVRVLNLILGSLVKDY
GFDASLAPINEIIYHMTLHQYPLVCSNKQSNPMRPHSTSEVLSAHSSTKDASTPGKEE
PRVTRSSTSADSTIITLPSIEVPNTYDDDRLKMLSAISLQIENSTFSKPFSTISK"
gene complement(<285241..>286914)
/gene="ICL1"
/locus_tag="YER065C"
/db_xref="GeneID:856794"
mRNA complement(<285241..>286914)
/gene="ICL1"
/locus_tag="YER065C"
/product="isocitrate lyase 1"
/transcript_id="NM_001178956.3"
/db_xref="GeneID:856794"
CDS complement(285241..286914)
/gene="ICL1"
/locus_tag="YER065C"
/EC_number="4.1.3.1"
/EC_number="4.1.3.30"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:8870676|PMID:9370278]"
/experiment="EXISTENCE:mutant phenotype:GO:0004451
isocitrate lyase activity [PMID:1551398]"
/experiment="EXISTENCE:mutant phenotype:GO:0006097
glyoxylate cycle [PMID:8878673]"
/note="Isocitrate lyase; catalyzes the formation of
succinate and glyoxylate from isocitrate, a key reaction
of the glyoxylate cycle; expression of ICL1 is induced by
growth on ethanol and repressed by growth on glucose"
/codon_start=1
/product="isocitrate lyase 1"
/protein_id="NP_010987.3"
/db_xref="GeneID:856794"
/db_xref="SGD:S000000867"
/translation="MPIPVGNTKNDFAALQAKLDADAAEIEKWWSDSRWSKTKRNYSA
RDIAVRRGTFPPIEYPSSVMARKLFKVLEKHHNEGTVSKTFGALDPVQISQMAKYLDT
IYISGWQCSSTASTSNEPGPDLADYPMDTVPNKVEHLFKAQLFHDRKQLEARSKAKSQ
EELDEMGAPIDYLTPIVADADAGHGGLTAVFKLTKMFIERGAAGIHMEDQTSTNKKCG
HMAGRCVIPVQEHVNRLVTIRMCADIMHSDLIVVARTDSEAATLISSTIDTRDHYFIV
GATNPNIEPFAEVLNDAIMSGASGQELADIEQKWCRDAGLKLFHEAVIDEIERSALSN
KQELIKKFTSKVGPLTETSHREAKKLAKEILGHEIFFDWELPRVREGLYRYRGGTQCS
IMRARAFAPYADLVWMESNYPDFQQAKEFAEGVKEKFPDQWLAYNLSPSFNWPKAMSV
DEQHTFIQRLGDLGYIWQFITLAGLHTNALAVHNFSRDFAKDGMKAYAQNVQQREMDD
GVDVLKHQKWSGAEYIDGLLKLAQGGVSATAAMGTGVTEDQFKENGVKK"
rep_origin 287552..287629
/note="ARS514; Autonomously Replicating Sequence"
/db_xref="SGD:S000077386"
repeat_region complement(287745..288062)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006894"
gene complement(288443..288524)
/gene="SUP19"
/locus_tag="YNCE0015C"
/db_xref="GeneID:856795"
tRNA complement(288443..288524)
/gene="SUP19"
/locus_tag="YNCE0015C"
/product="tRNA-Ser"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE
analysis; can mutate to suppress ochre nonsense mutations"
/db_xref="GeneID:856795"
/db_xref="SGD:S000006734"
gene <290242..>290799
/gene="RRT13"
/locus_tag="YER066W"
/db_xref="GeneID:856796"
mRNA <290242..>290799
/gene="RRT13"
/locus_tag="YER066W"
/product="Rrt13p"
/transcript_id="NM_001178957.1"
/db_xref="GeneID:856796"
CDS 290242..290799
/gene="RRT13"
/locus_tag="YER066W"
/note="hypothetical protein; non-essential gene identified
in a screen for mutants with decreased levels of rDNA
transcription"
/codon_start=1
/product="Rrt13p"
/protein_id="NP_010988.1"
/db_xref="GeneID:856796"
/db_xref="SGD:S000000868"
/translation="MKCLYILSGHTDRIYSTIYDHERKRCISASMDTTIRIWDLENIR
NNGECSYATNSASPCAKILGAMYTLRGHRALVGLLGLSDKFLVSASVDGSIRCWDANT
YFLKHFFDHTQLNTITALHVSDEVLVSGSEGLLNIYDLNSGLLVRSDTLSGADNVWNV
SFKDNTLVAAVERDKRNLLEILDFS"
gene <292066..>292551
/gene="RGI1"
/locus_tag="YER067W"
/db_xref="GeneID:856797"
mRNA <292066..>292551
/gene="RGI1"
/locus_tag="YER067W"
/product="Rgi1p"
/transcript_id="NM_001178958.1"
/db_xref="GeneID:856797"
CDS 292066..292551
/gene="RGI1"
/locus_tag="YER067W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:20567505]"
/experiment="EXISTENCE:mutant phenotype:GO:0006112 energy
reserve metabolic process [PMID:20567505]"
/note="Protein involved in energy metabolism under
respiratory conditions; abundance is increased upon
intracellular iron depletion or in response to DNA
replication stress; suppresses pro-apoptotic effect of
expressing human BAX in yeast cells; RGI1 has a paralog,
RGI2, that arose from the whole genome duplication"
/codon_start=1
/product="Rgi1p"
/protein_id="NP_010990.1"
/db_xref="GeneID:856797"
/db_xref="SGD:S000000869"
/translation="MTKKDKKEVKVQTVTTEDGETVKVFEDLQGFETFIANETEDDDF
DHLHCKLNYYPPFVLHESHEDPEKISDAANSHSKKFVRHLHQHIEKHLLKDIKQAVRK
PELKFHEKSKEETFDKITWHYGEETEYHGRPFKIDVQVVCTHEDAMVFVDYKTHPVGA
N"
gene <293050..>294813
/gene="MOT2"
/locus_tag="YER068W"
/gene_synonym="NOT4; SIG1"
/db_xref="GeneID:856799"
mRNA <293050..>294813
/gene="MOT2"
/locus_tag="YER068W"
/gene_synonym="NOT4; SIG1"
/product="CCR4-NOT core ubiquitin-protein ligase subunit
MOT2"
/transcript_id="NM_001178959.3"
/db_xref="GeneID:856799"
CDS 293050..294813
/gene="MOT2"
/locus_tag="YER068W"
/gene_synonym="NOT4; SIG1"
/EC_number="2.3.2.27"
/experiment="EXISTENCE:direct assay:GO:0000209 protein
polyubiquitination [PMID:19346402]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11889048]"
/experiment="EXISTENCE:direct assay:GO:0006513 protein
monoubiquitination [PMID:30609991]"
/experiment="EXISTENCE:direct assay:GO:0016567 protein
ubiquitination [PMID:16926149]"
/experiment="EXISTENCE:direct assay:GO:0022626 cytosolic
ribosome [PMID:30609991]"
/experiment="EXISTENCE:direct assay:GO:0032968 positive
regulation of transcription elongation by RNA polymerase
II [PMID:21406554]"
/experiment="EXISTENCE:direct assay:GO:0061630 ubiquitin
protein ligase activity
[PMID:30609991|PMID:16926149|PMID:19346402]"
/experiment="EXISTENCE:direct assay:GO:0070966
nuclear-transcribed mRNA catabolic process, no-go decay
[PMID:30609991]"
/experiment="EXISTENCE:genetic interaction:GO:0010498
proteasomal protein catabolic process [PMID:24261871]"
/experiment="EXISTENCE:mutant phenotype:GO:0000209 protein
polyubiquitination [PMID:19346402]"
/experiment="EXISTENCE:mutant phenotype:GO:0031087
deadenylation-independent decapping of nuclear-transcribed
mRNA [PMID:15706350]"
/experiment="EXISTENCE:mutant phenotype:GO:0032968
positive regulation of transcription elongation by RNA
polymerase II [PMID:11404327]"
/experiment="EXISTENCE:physical interaction:GO:0030015
CCR4-NOT core complex [PMID:10490603]"
/note="Ubiquitin-protein ligase subunit of the CCR4-NOT
complex; with Ubc4p, ubiquitinates nascent
polypeptide-associated complex subunits and histone
demethyase Jhd2p; CCR4-NOT has roles in transcription
regulation, mRNA degradation, and post-transcriptional
modifications; regulates levels of DNA Polymerase-{alpha}
to promote efficient and accurate DNA replication;
putative regulator of autophagy"
/codon_start=1
/product="CCR4-NOT core ubiquitin-protein ligase subunit
MOT2"
/protein_id="NP_010991.3"
/db_xref="GeneID:856799"
/db_xref="SGD:S000000870"
/translation="MMNPHVQENLQAIHNALSNFDTSFLSEDEEDYCPLCIEPMDITD
KNFFPCPCGYQICQFCYNNIRQNPELNGRCPACRRKYDDENVRYVTLSPEELKMERAK
LARKEKERKHREKERKENEYTNRKHLSGTRVIQKNLVYVVGINPPVPYEEVAPTLKSE
KYFGQYGKINKIVVNRKTPHSNNTTSEHYHHHSPGYGVYITFGSKDDAARCIAQVDGT
YMDGRLIKAAYGTTKYCSSYLRGLPCPNPNCMFLHEPGEEADSFNKRELHNKQQAQQQ
SGGTAFTRSGIHNNISTSTAGSNTNLLSENFTGTPSPAAMRAQLHHDSHTNAGTPVLT
PAPVPAGSNPWGVTQSATPVTSINLSKNSSSINLPTLNDSLGHHTTPTTENTITSTTT
TTNTNATSHSHGSKKKQSLAAEEYKDPYDALGNAVDFLDARLHSLSNYQKRPISIKSN
IIDEETYKKYPSLFSWDKIEASKKSDNTLANKLVEILAIKPIDYTASVVQFLQSVNVG
VNDNITITDNTKTPTQPIRLQTVSQQIQPPLNVSTPPPGIFGPQHKVPIQQQQMGDTS
SRNSSDLLNQLINGRKIIAGN"
gene <295410..>298001
/gene="ARG56"
/locus_tag="YER069W"
/db_xref="GeneID:856800"
mRNA <295410..>298001
/gene="ARG56"
/locus_tag="YER069W"
/product="bifunctional acetylglutamate
kinase/N-acetyl-gamma-glutamyl-phosphate reductase"
/transcript_id="NM_001178960.1"
/db_xref="GeneID:856800"
CDS 295410..298001
/gene="ARG56"
/locus_tag="YER069W"
/EC_number="1.2.1.38"
/EC_number="2.7.2.8"
/experiment="EXISTENCE:direct assay:GO:0003942
N-acetyl-gamma-glutamyl-phosphate reductase activity
[PMID:1851947]"
/experiment="EXISTENCE:direct assay:GO:0003991
acetylglutamate kinase activity [PMID:1851947]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:1851947]"
/experiment="EXISTENCE:mutant phenotype:GO:0006355
regulation of DNA-templated transcription [PMID:15486299]"
/note="Acetylglutamate kinase and
N-acetyl-gamma-glutamyl-phosphate reductase;
N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and
N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the
3rd step in arginine biosynthesis; synthesized as a
precursor which is processed in the mitochondrion to yield
mature NAGK and NAGSA; enzymes form a metabolon complex
with Arg2p; NAGK C-terminal domain stabilizes the enzymes,
slows catalysis and is involved in feed-back inhibition by
arginine"
/codon_start=1
/product="bifunctional acetylglutamate
kinase/N-acetyl-gamma-glutamyl-phosphate reductase"
/protein_id="NP_010992.1"
/db_xref="GeneID:856800"
/db_xref="SGD:S000000871"
/translation="MPSASLLVSTKRLNASKFQKFVSSLNKSTIAGFASVPLRAPPSV
AFTRKKVGYSKRYVSSTNGFSATRSTVIQLLNNISTKREVEQYLKYFTSVSQQQFAVI
KVGGAIISDNLHELASCLAFLYHVGLYPIVLHGTGPQVNGRLEAQGIEPDYIDGIRIT
DEHTMAVVRKCFLEQNLKLVTALEQLGVRARPITSGVFTADYLDKDKYKLVGNIKSVT
KEPIEASIKAGALPILTSLAETASGQMLNVNADVAAGELARVFEPLKIVYLNEKGGII
NGSTGEKISMINLDEEYDDLMKQSWVKYGTKLKIREIKELLDYLPRSSSVAIINVQDL
QKELFTDSGAGTMIRRGYKLVKRSSIGEFPSADALRKALQRDAGISSGKESVASYLRY
LENSDFVSYADEPLEAVAIVKKDTNVPTLDKFVCSDAAWLNNVTDNVFNVLRRDFPAL
QWVVSENDANIAWHFDKSQGSYLKGGKVLFWYGIDDINTISELVENFVKSCDTASTLN
SSASSGVFANKKSARSYSTRSTPRPEGVNTNPGRVALIGARGYTGKNLVSLINGHPYL
EVAHVSSRELKGQKLQDYTKSEIIYESLQIQDIRKLEEQNAVDFWVMALPNKVCEPFV
ETIQSVHGKSKIIDLSADHRFVSESDWAYGLPELNDRAKIANAAKIANPGCYATGSQL
TISPLTKYINGLPTVFGVSGYSGAGTKPSPKNDPKFLNNNLIPYALSDHIHEREISAR
IGHNVAFMPHVGQWFQGISLTVSIPIKKGSLSIDEIRKLYRNFYEDEKLVHVIDDIPL
VKDIEGTHGVVIGGFKLNDAEDRVVVCATIDNLLKGAATQCLQNINLAMGYGEYAGIP
ENKIIGV"
gene <298950..>301616
/gene="RNR1"
/locus_tag="YER070W"
/gene_synonym="CRT7; RIR1; SDS12"
/db_xref="GeneID:856801"
mRNA <298950..>301616
/gene="RNR1"
/locus_tag="YER070W"
/gene_synonym="CRT7; RIR1; SDS12"
/product="ribonucleotide-diphosphate reductase subunit
RNR1"
/transcript_id="NM_001178961.1"
/db_xref="GeneID:856801"
CDS 298950..301616
/gene="RNR1"
/locus_tag="YER070W"
/gene_synonym="CRT7; RIR1; SDS12"
/EC_number="1.17.4.1"
/experiment="EXISTENCE:direct assay:GO:0000166 nucleotide
binding [PMID:16537479]"
/experiment="EXISTENCE:direct assay:GO:0004748
ribonucleoside-diphosphate reductase activity, thioredoxin
disulfide as acceptor
[PMID:10716984|PMID:11893751|PMID:10535923]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:9315670|PMID:12732713|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005971
ribonucleoside-diphosphate reductase complex
[PMID:10716984]"
/experiment="EXISTENCE:direct assay:GO:0009263
deoxyribonucleotide biosynthetic process
[PMID:10535923|PMID:5459124]"
/experiment="EXISTENCE:mutant phenotype:GO:0004748
ribonucleoside-diphosphate reductase activity, thioredoxin
disulfide as acceptor [PMID:8552025]"
/note="Major isoform of large subunit of
ribonucleotide-diphosphate reductase; the RNR complex
catalyzes rate-limiting step in dNTP synthesis, regulated
by DNA replication and DNA damage checkpoint pathways via
localization of small subunits; relative distribution to
the nucleus increases upon DNA replication stress; RNR1
has a paralog, RNR3, that arose from the whole genome
duplication"
/codon_start=1
/product="ribonucleotide-diphosphate reductase subunit
RNR1"
/protein_id="NP_010993.1"
/db_xref="GeneID:856801"
/db_xref="SGD:S000000872"
/translation="MYVYKRDGRKEPVQFDKITARISRLCYGLDPKHIDAVKVTQRII
SGVYEGVTTIELDNLAAETCAYMTTVHPDYATLAARIAISNLHKQTTKQFSKVVEDLY
RYVNAATGKPAPMISDDVYNIVMENKDKLNSAIVYDRDFQYSYFGFKTLERSYLLRIN
GQVAERPQHLIMRVALGIHGRDIEAALETYNLMSLKYFTHASPTLFNAGTPKPQMSSC
FLVAMKEDSIEGIYDTLKECALISKTAGGIGLHIHNIRSTGSYIAGTNGTSNGLIPMI
RVFNNTARYVDQGGNKRPGAFALYLEPWHADIFDFIDIRKNHGKEEIRARDLFPALWI
PDLFMKRVEENGTWTLFSPTSAPGLSDCYGDEFEALYTRYEKEGRGKTIKAQKLWYSI
LEAQTETGTPFVVYKDACNRKSNQKNLGVIKSSNLCCEIVEYSAPDETAVCNLASVAL
PAFIETSEDGKTSTYNFKKLHEIAKVVTRNLNRVIDRNYYPVEEARKSNMRHRPIALG
VQGLADTFMLLRLPFDSEEARLLNIQIFETIYHASMEASCELAQKDGPYETFQGSPAS
QGILQFDMWDQKPYGMWDWDTLRKDIMKHGVRNSLTMAPMPTASTSQILGYNECFEPV
TSNMYSRRVLSGEFQVVNPYLLRDLVDLGIWDEGMKQYLITQNGSIQGLPNVPQELKD
LYKTVWEISQKTIINMAADRSVYIDQSHSLNLFLRAPTMGKLTSMHFYGWKKGLKTGM
YYLRTQAASAAIQFTIDQKIADQATENVADISNLKRPSYMPSSASYAASDFVPAAVTA
NATIPSLDSSSEASREASPAPTGSHSLTKGMAELNVQESKVEVPEVPAPTKNEEKAAP
IVDDEETEFDIYNSKVIACAIDNPEACEMCSG"
gene complement(<301947..>302327)
/gene="TDA2"
/locus_tag="YER071C"
/db_xref="GeneID:856802"
mRNA complement(<301947..>302327)
/gene="TDA2"
/locus_tag="YER071C"
/product="Tda2p"
/transcript_id="NM_001178962.3"
/db_xref="GeneID:856802"
CDS complement(301947..302327)
/gene="TDA2"
/locus_tag="YER071C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0030479 actin
cortical patch [PMID:29467252|PMID:28706108]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807]"
/experiment="EXISTENCE:direct assay:GO:0051015 actin
filament binding [PMID:29467252]"
/experiment="EXISTENCE:physical interaction:GO:0030837
negative regulation of actin filament polymerization
[PMID:29467252]"
/experiment="EXISTENCE:physical interaction:GO:0110131
Aim21-Tda2 complex [PMID:29467252|PMID:28706108]"
/note="Subunit of a complex that associates with actin
filaments; forms a complex with Aim21p that inhibits
barbed end F-actin assembly; elevates actin monomer pools
to increase endocytotic efficiency and to regulate the
distribution of actin between cables and patches;
Aim21p/Tda2p forms a larger complex with actin capping
proteins Cap1p and Cap2p; TcTex1-type dynein light chain
family member; null mutant is sensitive to expression of
the top1-T722A allele"
/codon_start=1
/product="Tda2p"
/protein_id="NP_010994.3"
/db_xref="GeneID:856802"
/db_xref="SGD:S000000873"
/translation="MSMQIEIKDGRSDNSPLPERKLVTLIQESYDSLKDDNEINLSTE
STSNLLIKLVLEKLEKHSSLYKYIASVTTLNIEGLNEENANFSLKNDIGASWESKKDG
IFNYKLEDKNNNECYLITILWLHK"
gene <302806..>303195
/gene="VTC1"
/locus_tag="YER072W"
/gene_synonym="NRF1; PHM4"
/db_xref="GeneID:856803"
mRNA <302806..>303195
/gene="VTC1"
/locus_tag="YER072W"
/gene_synonym="NRF1; PHM4"
/product="Vtc1p"
/transcript_id="NM_001178963.1"
/db_xref="GeneID:856803"
CDS 302806..303195
/gene="VTC1"
/locus_tag="YER072W"
/gene_synonym="NRF1; PHM4"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:20844764]"
/experiment="EXISTENCE:direct assay:GO:0005774 vacuolar
membrane [PMID:12584253]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:17079729]"
/experiment="EXISTENCE:direct assay:GO:0007034 vacuolar
transport [PMID:17079729]"
/experiment="EXISTENCE:direct assay:GO:0016237
microautophagy [PMID:17079729]"
/experiment="EXISTENCE:direct assay:GO:0031965 nuclear
membrane [PMID:27831485]"
/experiment="EXISTENCE:mutant phenotype:GO:0006797
polyphosphate metabolic process
[PMID:19390046|PMID:11102525]"
/experiment="EXISTENCE:mutant phenotype:GO:0006799
polyphosphate biosynthetic process [PMID:27252384]"
/experiment="EXISTENCE:mutant phenotype:GO:0007034
vacuolar transport [PMID:17079729]"
/experiment="EXISTENCE:mutant phenotype:GO:0016237
microautophagy [PMID:17079729]"
/experiment="EXISTENCE:mutant phenotype:GO:0042144 vacuole
fusion, non-autophagic [PMID:27252384|PMID:11823419]"
/experiment="EXISTENCE:physical interaction:GO:0033254
vacuolar transporter chaperone complex [PMID:11823419]"
/note="Regulatory subunit of vacuolar transporter
chaperone (VTC) complex; VTC complex is involved in
membrane trafficking, vacuolar polyphosphate accumulation,
microautophagy and non-autophagic vacuolar fusion;
important regulator of substrate invagination from the
vacuolar membrane; also binds mRNA; targeted to vacuole
via AP-3 pathway; protein abundance increases in response
to DNA replication stress"
/codon_start=1
/product="Vtc1p"
/protein_id="NP_010995.1"
/db_xref="GeneID:856803"
/db_xref="SGD:S000000874"
/translation="MSSAPLLQRTPGKKIALPTRVEPKVFFANERTFLSWLNFTVMLG
GLGVGLLNFGDKIGRVSAGLFTFVAMGTMIYALVTYHWRAAAIRRRGSGPYDDRLGPT
LLCFFLLVAVIINFILRLKYNDANTKL"
gene <304030..>305592
/gene="ALD5"
/locus_tag="YER073W"
/db_xref="GeneID:856804"
mRNA <304030..>305592
/gene="ALD5"
/locus_tag="YER073W"
/product="aldehyde dehydrogenase (NAD(P)(+)) ALD5"
/transcript_id="NM_001178964.2"
/db_xref="GeneID:856804"
CDS 304030..305592
/gene="ALD5"
/locus_tag="YER073W"
/EC_number="1.2.1.4"
/experiment="EXISTENCE:direct assay:GO:0004029 aldehyde
dehydrogenase (NAD+) activity [PMID:9473035]"
/experiment="EXISTENCE:direct assay:GO:0004030 aldehyde
dehydrogenase [NAD(P)+] activity [PMID:9473035]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:24769239|PMID:16823961|PMID:14576278|PMID:10585550]"
/experiment="EXISTENCE:mutant phenotype:GO:0019413 acetate
biosynthetic process [PMID:15256563]"
/note="Mitochondrial aldehyde dehydrogenase; involved in
regulation or biosynthesis of electron transport chain
components and acetate formation; activated by K+;
utilizes NADP+ as the preferred coenzyme; constitutively
expressed"
/codon_start=1
/product="aldehyde dehydrogenase (NAD(P)(+)) ALD5"
/protein_id="NP_010996.2"
/db_xref="GeneID:856804"
/db_xref="SGD:S000000875"
/translation="MLSRTRAAAPNSRIFTRSLLRLYSQAPLRVPITLPNGFTYEQPT
GLFINGEFVASKQKKTFDVINPSNEEKITTVYKAMEDDVDEAVAAAKKAFETKWSIVE
PEVRAKALFNLADLVEKHQETLAAIESMDNGKSLFCARGDVALVSKYLRSCGGWADKI
YGNVIDTGKNHFTYSIKEPLGVCGQIIPWNFPLLMWSWKIGPALATGNTVVLKPAETT
PLSALFASQLCQEAGIPAGVVNILPGSGRVVGERLSAHPDVKKIAFTGSTATGRHIMK
VAADTVKKVTLELGGKSPNIVFADADLDKAVKNIAFGIFYNSGEVCCAGSRIYIQDTV
YEEVLQKLKDYTESLKVGDPFDEEVFQGAQTSDKQLHKILDYVDVAKSEGARLVTGGA
RHGSKGYFVKPTVFADVKEDMRIVKEEVFGPIVTVSKFSTVDEVIAMANDSQYGLAAG
IHTNDINKAVDVSKRVKAGTVWINTYNNFHQNVPFGGFGQSGIGREMGEAALSNYTQT
KSVRIAIDKPIR"
gene <306323..>307196
/gene="RPS24A"
/locus_tag="YER074W"
/gene_synonym="RPS24EA"
/db_xref="GeneID:856805"
mRNA join(<306323..306325,306792..>307196)
/gene="RPS24A"
/locus_tag="YER074W"
/gene_synonym="RPS24EA"
/product="40S ribosomal protein eS24 RPS24A"
/transcript_id="NM_001178965.3"
/db_xref="GeneID:856805"
CDS join(306323..306325,306792..307196)
/gene="RPS24A"
/locus_tag="YER074W"
/gene_synonym="RPS24EA"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:genetic interaction:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16246728]"
/note="Protein component of the small (40S) ribosomal
subunit; homologous to mammalian ribosomal protein S24, no
bacterial homolog; RPS24A has a paralog, RPS24B, that
arose from the whole genome duplication"
/codon_start=1
/product="40S ribosomal protein eS24 RPS24A"
/protein_id="NP_010997.3"
/db_xref="GeneID:856805"
/db_xref="SGD:S000000876"
/translation="MSDAVTIRTRKVISNPLLARKQFVVDVLHPNRANVSKDELREKL
AEVYKAEKDAVSVFGFRTQFGGGKSVGFGLVYNSVAEAKKFEPTYRLVRYGLAEKVEK
ASRQQRKQKKNRDKKIFGTGKRLAKKVARRNAD"
gene <307653..>308123
/gene="YOS1"
/locus_tag="YER074W-A"
/db_xref="GeneID:856806"
mRNA join(<307653..307746,307849..307956,308068..>308123)
/gene="YOS1"
/locus_tag="YER074W-A"
/product="Yos1p"
/transcript_id="NM_001184512.1"
/db_xref="GeneID:856806"
CDS join(307653..307746,307849..307956,308068..308123)
/gene="YOS1"
/locus_tag="YER074W-A"
/experiment="EXISTENCE:direct assay:GO:0000139 Golgi
membrane [PMID:15659647]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:15659647]"
/experiment="EXISTENCE:direct assay:GO:0030134
COPII-coated ER to Golgi transport vesicle
[PMID:15659647]"
/experiment="EXISTENCE:mutant phenotype:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:15659647]"
/experiment="EXISTENCE:physical interaction:GO:0000139
Golgi membrane [PMID:15659647]"
/experiment="EXISTENCE:physical interaction:GO:0005789
endoplasmic reticulum membrane [PMID:15659647]"
/experiment="EXISTENCE:physical interaction:GO:0030134
COPII-coated ER to Golgi transport vesicle
[PMID:15659647]"
/note="Integral membrane protein required for ER to Golgi
transport; localized to the Golgi, the ER, and COPII
vesicles; interacts with Yip1p and Yif1p"
/codon_start=1
/product="Yos1p"
/protein_id="NP_219496.2"
/db_xref="GeneID:856806"
/db_xref="SGD:S000007651"
/translation="MVLFGLGRLFYVILLLINAVAVLSEERFLRRIGLGRSNDETPVF
GQDQNTTKSKVVQLIGAVQTLLRIPLIGINILVIVYELLLG"
gene complement(<308413..>311199)
/gene="PTP3"
/locus_tag="YER075C"
/db_xref="GeneID:856807"
mRNA complement(<308413..>311199)
/gene="PTP3"
/locus_tag="YER075C"
/product="tyrosine protein phosphatase PTP3"
/transcript_id="NM_001178966.2"
/db_xref="GeneID:856807"
CDS complement(308413..311199)
/gene="PTP3"
/locus_tag="YER075C"
/EC_number="3.1.3.48"
/experiment="EXISTENCE:direct assay:GO:0004725 protein
tyrosine phosphatase activity [PMID:9224718]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:17952059]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:10523653|PMID:17952059]"
/experiment="EXISTENCE:direct assay:GO:1903138 negative
regulation of cell integrity MAPK cascade [PMID:9224718]"
/experiment="EXISTENCE:genetic interaction:GO:0043937
regulation of sporulation [PMID:10679022]"
/experiment="EXISTENCE:genetic interaction:GO:0071852
fungal-type cell wall organization or biogenesis
[PMID:10523653]"
/experiment="EXISTENCE:mutant phenotype:GO:0005737
cytoplasm [PMID:10817757]"
/experiment="EXISTENCE:mutant phenotype:GO:0071474
cellular hyperosmotic response [PMID:10817757]"
/experiment="EXISTENCE:mutant phenotype:GO:0071507
pheromone response MAPK cascade [PMID:9224718]"
/experiment="EXISTENCE:mutant phenotype:GO:0140311 protein
sequestering activity [PMID:10817757]"
/note="Phosphotyrosine-specific protein phosphatase; major
role in the pheromone adaptive response through
dephosphorylation of the Fus3p MAPK with a minor role by
Ptp2p and Msg5p; minor role in the inactivation of Hog1p
MAPK during osmolarity sensing with major role by Ptp2p;
dephosphorylates and regulates the localization of Hog1p;
minor role in the cell wall integrity pathway through
tyrosine dephosphorylation of the MAPK Slt2p while Ptp2p
plays a major role; localizes to the cytoplasm"
/codon_start=1
/product="tyrosine protein phosphatase PTP3"
/protein_id="NP_010998.2"
/db_xref="GeneID:856807"
/db_xref="SGD:S000000877"
/translation="MKDSVDCPSILPTDRTSVLSETSTLVGSSSHVYSRHAPMNSYHN
SMNSNIYHSPKASSPLVSYKTSSPVLLKRATAPVLPSFKPKEQRYNKPQGCSLITAVE
LGKIIETLPDEKVLLLDVRPFTEHAKSIITNSIHVCLPSTLLRRKNFTFSKLLDNLTP
SEQSVLKSKLAIDNLRIIIYDSTANQTESSVSLPCYGIASKLIEFDTNVKKTVSILMC
GFPQFKILFPDHINTNTFNSDCISSAEPKSPKTNLMNSLHNTAPHMTATTPLSSPQMN
LKLKVPDDSRSDHSNFSSSPSPRNVLSDSPMSSSSPISALFKFQLPAPQTNINQMFKF
SQNEEIMGLETYLSAVNIKEEHERWYNNDSAKKSLQNFQFPKNQNSLEKDTNKDKLGF
QIRYENLSKNYEKEVIDSVIPEWFQHLMSIPKIELVSQFQKLDFLEKRRLNHSVSFRK
KENSFILEKPSSYPEQLTSTSSSTIMPPKFPDVNKVQKRSHSQPIFTQYSKYKSMLSL
ESDSDSESDDVIISSGVELGAKNRYKDIFPYEHSRVILKKGLQSSKGIKHSHSTSDGG
ILDNYINANYLSLPRFSVEQNSSFQTTTTTTRRVRYIATQAPMPSTVHDFYTCILNNG
VPLVLSLTNDFENGIEKCYRYWQEGNYNGIHVKLLEKKILKMPSTTSMRKNTMGTQNS
SLYSAGVQGNSSNYSTDNDNDNDNNNNNNNNSNIAVTAAACDDDDDDDDDAILIRKIL
LTYHDQEKPYELLQIQVKNWPDLGTLLNPISILQAINVKNHIIDTLFARNYYQNDQLP
TILVHCSAGCGRTGTLCTIDSILSNFEMFEMLQKEFVKLKYPAKLFDPISWTINIFRK
QRISMVQNINQFIFIYDCLLFYFRLRLDDITERTDGDGSNKDNISLSALIEQIEKLEI
LQTFVDDKLKELPQ"
gene complement(312023..312095)
/locus_tag="YNCE0016C"
/db_xref="GeneID:856808"
tRNA complement(312023..312095)
/locus_tag="YNCE0016C"
/product="tRNA-Ala"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE
analysis; one of 5 nuclear tRNA genes containing the
tDNA-anticodon TGC (mature tRNA may be UGC or may contain
modified bases), decodes GCA and probably GCG codons into
alanine, one of 16 nuclear tRNAs for alanine"
/db_xref="GeneID:856808"
/db_xref="SGD:S000006522"
repeat_region 312274..312445
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006914"
gene complement(<312590..>313498)
/locus_tag="YER076C"
/db_xref="GeneID:856809"
mRNA complement(<312590..>313498)
/locus_tag="YER076C"
/product="uncharacterized protein"
/transcript_id="NM_001178967.3"
/db_xref="GeneID:856809"
CDS complement(312590..313498)
/locus_tag="YER076C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/note="hypothetical protein; the authentic, non-tagged
protein is detected in highly purified mitochondria in
high-throughput studies; analysis of HA-tagged protein
suggests a membrane localization"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_010999.3"
/db_xref="GeneID:856809"
/db_xref="SGD:S000000878"
/translation="MTSGYYTVSFFYAILLLCACTRAEIYLTGGESQGLPSGFWQMDD
DLAIAPVSMVEFYQTIGLTANGTVPESFNKRDATEYPNIISNITTQAANFTQHSLVEQ
LQNDVTAISISNAINVAVDGSVETPADLQYNRNQETGESTLCRAKFYGVEVRTWSRLY
NRAVSSTTLTTNLNSYIAQWVAWAVHGSGDKKFCGSQEFTNIFFDGQEGWSLFVKTWS
TNSSCDITASEGNLTCAVRVSVSSMHNHGKTAFCVTYSHGDSWRAELRVVANDAWSHY
YPWSIDCPEVDKNNMAINDCFDQAQG"
gene complement(<314534..>316600)
/gene="MRX1"
/locus_tag="YER077C"
/db_xref="GeneID:856810"
mRNA complement(<314534..>316600)
/gene="MRX1"
/locus_tag="YER077C"
/product="Mrx1p"
/transcript_id="NM_001178968.1"
/db_xref="GeneID:856810"
CDS complement(314534..316600)
/gene="MRX1"
/locus_tag="YER077C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095|PMID:24769239]"
/note="Protein that associates with mitochondrial
ribosome; green fluorescent protein (GFP)-fusion protein
localizes to the mitochondrion; null mutation results in a
decrease in plasma membrane electron transport"
/codon_start=1
/product="Mrx1p"
/protein_id="NP_011000.1"
/db_xref="GeneID:856810"
/db_xref="SGD:S000000879"
/translation="MGLKITKGQLRTKDLNQSSSKSSQSSRIGVDTCIFTRMLPRINT
AINLTEHLLRRSFHSLTNLQKTQVKERLHELERHGFILNKTSKQLERINSKKRRQLKK
LQKTAYPKDQAFHILRKFHKINNEALADTKLGPTSQSDLKFLSLTKDKRLFYTILGVN
GEQLRDSKLIANDVQKFLKRGQLEKAVFLARLAKKKGVVGMNLIMKYYIEVVQSQQSA
VDIFNWRKKWGVPIDQHSITILFNGLSKQENLVSKKYGELVLKTIDSLCDKNELTEIE
YNTALAALINCTDETLVFKLLNKKCPGLKKDSITYTLMIRSCTRIADEKRFMVVLNDL
MNKIPDYCVDSKLLFEYCEVICSQKSPKIEKQGMGLWALCEYFQFDKTIFKKYLTQSD
FPTLVPLSHWNINKPFPLNKHVVGLFMNYCLKNKEYDLAMEIFKTLEAQNNQMLDQSI
YHKYMETVITTRPITCGDECLDIYERVASSAQISITRRTLILVYNAFQRQSLKAVINK
DASNAEATLHKIRGFIDSVEATYSSKLNGKVYRFNSWKFLFPIVKNLNMNDKVSTVEL
KSILDEYLKSLLNGEFGKEFKASIEDKRFVTLEGIRLVKVLTERIKLPSLDSEEIASL
KGTERKKFLARRHLLRLKQILLEDLADIEGNSRRKGDSENTSTSEERIMEDLAELILE
TSYDKF"
rep_origin 316600..316807
/note="ARS515; Autonomously Replicating Sequence"
/db_xref="SGD:S000077387"
gene complement(<316807..>318342)
/gene="ICP55"
/locus_tag="YER078C"
/db_xref="GeneID:856811"
mRNA complement(<316807..>318342)
/gene="ICP55"
/locus_tag="YER078C"
/product="aminopeptidase"
/transcript_id="NM_001178969.1"
/db_xref="GeneID:856811"
CDS complement(316807..318342)
/gene="ICP55"
/locus_tag="YER078C"
/EC_number="3.4.11.26"
/experiment="EXISTENCE:direct assay:GO:0004177
aminopeptidase activity [PMID:19720832]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:19720832]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:14562095|PMID:24769239|PMID:16823961|PMID:19720832]"
/experiment="EXISTENCE:genetic interaction:GO:0016485
protein processing [PMID:19837041]"
/experiment="EXISTENCE:mutant phenotype:GO:0004177
aminopeptidase activity [PMID:19837041|PMID:19720832]"
/experiment="EXISTENCE:mutant phenotype:GO:0016485 protein
processing [PMID:19720832|PMID:19837041]"
/experiment="EXISTENCE:mutant phenotype:GO:0050821 protein
stabilization [PMID:19837041]"
/note="Mitochondrial aminopeptidase; cleaves N termini of
at least 38 imported proteins after cleavage by the
mitochondrial processing peptidase (MPP), thereby
increasing their stability; member of aminopeptidase P
family; exists in rapid equilibrium between monomer and
dimer, with dimer being active species; ortholog of human
gene XPNPEP3, which is mutated in rare hereditary kidney
disease similar to nephronophthisis"
/codon_start=1
/product="aminopeptidase"
/protein_id="NP_011001.1"
/db_xref="GeneID:856811"
/db_xref="SGD:S000000880"
/translation="MLHRINPVRFSMQSCQRYFSKLVSPLEQHKSNTFTNRVRIPIEA
GQPLHETRPFLIKSGELTPGISALEYYERRIRLAETLPPKSCVILAGNDIQFASGAVF
YPFQQENDLFYLSGWNEPNSVMILEKPTDSLSDTIFHMLVPPKDAFAEKWEGFRSGVY
GVQEIFNADESASINDLSKYLPKIINRNDFIYFDMLSTSNPSSSNFKHIKSLLDGSGN
SNRSLNSIANKTIKPISKRIAEFRKIKSPQELRIMRRAGQISGRSFNQAFAKRFRNER
TLDSFLHYKFISGGCDKDAYIPVVATGSNSLCIHYTRNDDVMFDDEMVLVDAAGSLGG
YCADISRTWPNSGKFTDAQRDLYEAVLNVQRDCIKLCKASNNYSLHDIHEKSITLMKQ
ELKNLGIDKVSGWNVEKLYPHYIGHNLGLDVHDVPKVSRYEPLKVGQVITIEPGLYIP
NEESFPSYFRNVGIRIEDDIAIGEDTYTNLTVEAVKEIDDLENVMQNGLSTKFEEDQV
APL"
gene <318646..>318810
/locus_tag="YER078W-A"
/db_xref="GeneID:1466536"
mRNA <318646..>318810
/locus_tag="YER078W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184548.3"
/db_xref="GeneID:1466536"
CDS 318646..318810
/locus_tag="YER078W-A"
/note="hypothetical protein; identified by fungal homology
and RT-PCR"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878068.3"
/db_xref="GeneID:1466536"
/db_xref="SGD:S000028546"
/translation="MVRLSYLRLILPPCRLSELSSLAILYQPVIPILISTITFQHFFK
CVHTPCNVYI"
gene <318920..>319552
/locus_tag="YER079W"
/db_xref="GeneID:856812"
mRNA <318920..>319552
/locus_tag="YER079W"
/product="uncharacterized protein"
/transcript_id="NM_001178970.1"
/db_xref="GeneID:856812"
CDS 318920..319552
/locus_tag="YER079W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011002.1"
/db_xref="GeneID:856812"
/db_xref="SGD:S000000881"
/translation="MPDSSHSISSKDVASAISLYDQSIYTNNRSTNLDLDQRSMSPSN
IASGEDRITRTNSGCSITSGASMIATKDGIQGINVKRDGIPKYSLNLLNSMVRKQYDH
NNGTKSPTPKTSNMVDPKNKKKNKKKKNDKDDKYKVSHDQTEKFYKLNTTSNSNLTSD
STTSLSDQFYFQKSNADSAPLDNANYPLSDHSPSLNSMDNTTKHSSNVHT"
gene <319963..>321846
/gene="AIM9"
/locus_tag="YER080W"
/gene_synonym="FMP29"
/db_xref="GeneID:856813"
mRNA <319963..>321846
/gene="AIM9"
/locus_tag="YER080W"
/gene_synonym="FMP29"
/product="Aim9p"
/transcript_id="NM_001178971.1"
/db_xref="GeneID:856813"
CDS 319963..321846
/gene="AIM9"
/locus_tag="YER080W"
/gene_synonym="FMP29"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:14576278|PMID:24769239|PMID:16823961|PMID:14562095]"
/note="hypothetical protein; the authentic, non-tagged
protein is detected in highly purified mitochondria in
high-throughput studies; null mutant displays elevated
frequency of mitochondrial genome loss"
/codon_start=1
/product="Aim9p"
/protein_id="NP_011003.1"
/db_xref="GeneID:856813"
/db_xref="SGD:S000000882"
/translation="MIRYTVAGHSRRCVVGASKRVGAIKCITVAATKRFISNKPNEVF
TKLTNDNDPKRDAFFKYTWGSWLKNDKQEKEKRFTKFSIEGLNRILNDIYIQSNEMAK
APDGKILPPVFNKNLTVSLVNNVVPKNIGKINPNEKVQVTTLSSIHEGKHHRIYKVDT
NLNKAFILRIPYPLENENTLSYRIRSEVATMDFADLKLGIKVPKIFCYGVNSLNPVRQ
PFVLQEFIEGELLMKDWDPLIEDGSSNQKKYDNVIKQVSDFQSKLVSLKLNAFGSIYF
NNDLKDGNEKEFVKEDIYDGETNPDLQNRWKIGPSVERCLWRHKSHLDFHKQMKPFLG
PWPKKSPMDIIKNTGLLEAENAKTRIAMKEAGSSAELMYPRTLKEQITTYENLAKIAP
DLFNVKTKAIPNMQELLSPRLFHPDLDPMNIIVNKEAQEAYLLDFEGACTKPFILQNS
PQFIAYDGPKIYDLKEDITDFDKLSEAEKVQYQFMYKRTRNQHQWEKKLNDNNPKLIT
AVAPPVKLLRSPYIAAVERKTEEEYLLIDESLLQLKEVWDIFAQNELVNQKKFPLNYS
KEDIERHVEDLQKLHEKLISTPFAATQGWIPQDMFDQLLNSGSIVKQENGDYTVKQPE
ATK"
gene 322212..322762
/gene="SRG1"
/locus_tag="YNCE0017W"
/db_xref="GeneID:9164885"
ncRNA 322212..322762
/ncRNA_class="other"
/gene="SRG1"
/locus_tag="YNCE0017W"
/product="SRG1"
/experiment="EXISTENCE:curator inference:GO:0005634
nucleus [PMID:15175754]"
/experiment="EXISTENCE:expression pattern:GO:0045892
negative regulation of DNA-templated transcription
[PMID:15175754]"
/experiment="EXISTENCE:mutant phenotype:GO:0045892
negative regulation of DNA-templated transcription
[PMID:15175754]"
/note="Non-protein-coding RNA that regulates the
transcription of SER3; expression of SRG1 RNA represses
expression of its neighboring gene SER3 via a
transcription-interference mechanism"
/transcript_id="NR_132168.1"
/db_xref="GeneID:9164885"
/db_xref="SGD:S000029010"
gene <322686..>324095
/gene="SER3"
/locus_tag="YER081W"
/db_xref="GeneID:856814"
mRNA <322686..>324095
/gene="SER3"
/locus_tag="YER081W"
/product="phosphoglycerate dehydrogenase SER3"
/transcript_id="NM_001178972.3"
/db_xref="GeneID:856814"
CDS 322686..324095
/gene="SER3"
/locus_tag="YER081W"
/EC_number="1.1.1.95"
/EC_number="1.1.1.399"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0047545
(S)-2-hydroxyglutarate dehydrogenase activity
[PMID:26774271]"
/experiment="EXISTENCE:genetic interaction:GO:0047545
(S)-2-hydroxyglutarate dehydrogenase activity
[PMID:26774271]"
/experiment="EXISTENCE:mutant phenotype:GO:0004617
phosphoglycerate dehydrogenase activity [PMID:12525494]"
/experiment="EXISTENCE:mutant phenotype:GO:0006564
L-serine biosynthetic process [PMID:12525494]"
/experiment="EXISTENCE:mutant phenotype:GO:0047545
(S)-2-hydroxyglutarate dehydrogenase activity
[PMID:26774271]"
/note="3-phosphoglycerate dehydrogenase and
alpha-ketoglutarate reductase; 3PG dehydrogenase that
catalyzes the first step in serine and glycine
biosynthesis; alpha-ketoglutarate reductase, converting
alpha-ketoglutarate to D-2-hydroxyglutarate (D-2HG);
directly phosphorylated by TORC1 in a Pib2-dependent
manner; localizes to the cytoplasm; ER3 has a paralog,
SER33, that arose from the whole genome duplication"
/codon_start=1
/product="phosphoglycerate dehydrogenase SER3"
/protein_id="NP_011004.3"
/db_xref="GeneID:856814"
/db_xref="SGD:S000000883"
/translation="MTSIDINNLQNTFQQAMNMSGSPGAVCTSPTQSFMNTVPQRLNA
VKHPKILKPFSTGDMKILLLENVNQTAITIFEEQGYQVEFYKSSLPEEELIEKIKDVH
AIGIRSKTRLTSNVLQHAKNLVCIGCFCIGTNQVDLDYATSRGIAVFNSPFSNSRSVA
ELVIAEIISLARQLGDRSIELHTGTWNKVAARCWEVRGKTLGIIGYGHIGSQLSVLAE
AMGLHVLYYDIVTIMALGTARQVSTLDELLNKSDFVTLHVPATPETEKMLSAPQFAAM
KDGAYVINASRGTVVDIPSLIQAVKANKIAGAALDVYPHEPAKNGEGSFNDELNSWTS
ELVSLPNIILTPHIGGSTEEAQSSIGIEVATALSKYINEGNSVGSVNFPEVALKSLSY
DQENTVRVLYIHQNVPGVLKTVNDILSNHNIEKQFSDSNGEIAYLMADISSVDQSDIK
DIYEQLNQTSAKISIRLLY"
gene complement(<324272..>325936)
/gene="UTP7"
/locus_tag="YER082C"
/gene_synonym="KRE31"
/db_xref="GeneID:856815"
mRNA complement(<324272..>325936)
/gene="UTP7"
/locus_tag="YER082C"
/gene_synonym="KRE31"
/product="Utp7p"
/transcript_id="NM_001178973.1"
/db_xref="GeneID:856815"
CDS complement(324272..325936)
/gene="UTP7"
/locus_tag="YER082C"
/gene_synonym="KRE31"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:12068309|PMID:12150911]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/experiment="EXISTENCE:direct assay:GO:0032040
small-subunit processome [PMID:12068309]"
/experiment="EXISTENCE:mutant phenotype:GO:0000447
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from
5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:15489292|PMID:15590835]"
/experiment="EXISTENCE:mutant phenotype:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12068309]"
/experiment="EXISTENCE:mutant phenotype:GO:0000472
endonucleolytic cleavage to generate mature 5'-end of
SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:15489292|PMID:15590835]"
/experiment="EXISTENCE:mutant phenotype:GO:0000480
endonucleolytic cleavage in 5'-ETS of tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:15590835|PMID:15489292]"
/note="Nucleolar protein; component of the small subunit
(SSU) processome containing the U3 snoRNA that is involved
in processing of pre-18S rRNA"
/codon_start=1
/product="Utp7p"
/protein_id="NP_011005.1"
/db_xref="GeneID:856815"
/db_xref="SGD:S000000884"
/translation="MGHKKNGHRRQIKERENQNKFERSTYTNNAKNNHTQTKDKKLRA
GLKKIDEQYKKAVSSAAATDYLLPESNGYLEPENELEKTFKVQQSEIKSSVDVSTANK
ALDLSLKEFGPYHIKYAKNGTHLLITGRKGHVASMDWRKGQLRAELFLNETCHSATYL
QNEQYFAVAQKKYTFIYDHEGTELHRLKQHIEARHLDFLPYHYLLVTAGETGWLKYHD
VSTGQLVSELRTKAGPTMAMAQNPWNAVMHLGHSNGTVSLWSPSMPEPLVKLLSARGP
VNSIAIDRSGYYMATTGADRSMKIWDIRNFKQLHSVESLPTPGTNVSISDTGLLALSR
GPHVTLWKDALKLSGDSKPCFGSMGGNPHRNTPYMSHLFAGNKVENLGFVPFEDLLGV
GHQTGITNLIVPGAGEANYDALELNPFETKKQRQEQEVRTLLNKLPADTITLDPNSIG
SVDKRSSTIRLNAKDLAQTTMDANNKAKTNSDIPDVKPDVKGKNSGLRSFLRKKTQNV
IDERKLRVQKQLDKEKNIRKRNHQIKQGLISEDHKDVIEEALSRFG"
gene complement(<326174..>327031)
/gene="GET2"
/locus_tag="YER083C"
/gene_synonym="HUR2; RMD7"
/db_xref="GeneID:856817"
mRNA complement(<326174..>327031)
/gene="GET2"
/locus_tag="YER083C"
/gene_synonym="HUR2; RMD7"
/product="GET complex subunit GET2"
/transcript_id="NM_001178974.1"
/db_xref="GeneID:856817"
CDS complement(326174..327031)
/gene="GET2"
/locus_tag="YER083C"
/gene_synonym="HUR2; RMD7"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:36640319|PMID:25043001]"
/experiment="EXISTENCE:direct assay:GO:0006890 retrograde
vesicle-mediated transport, Golgi to endoplasmic reticulum
[PMID:16269340]"
/experiment="EXISTENCE:direct assay:GO:0008320 protein
transmembrane transporter activity [PMID:36640319]"
/experiment="EXISTENCE:direct assay:GO:0097051
establishment of protein localization to endoplasmic
reticulum membrane [PMID:36640319]"
/experiment="EXISTENCE:genetic interaction:GO:0006890
retrograde vesicle-mediated transport, Golgi to
endoplasmic reticulum [PMID:16269340]"
/experiment="EXISTENCE:genetic interaction:GO:0043495
protein-membrane adaptor activity [PMID:18724936]"
/experiment="EXISTENCE:genetic interaction:GO:0043529 GET
complex [PMID:16269340]"
/experiment="EXISTENCE:genetic interaction:GO:0071816
tail-anchored membrane protein insertion into ER membrane
[PMID:18724936]"
/experiment="EXISTENCE:mutant phenotype:GO:0000423
mitophagy [PMID:29673596]"
/experiment="EXISTENCE:mutant phenotype:GO:0006890
retrograde vesicle-mediated transport, Golgi to
endoplasmic reticulum [PMID:16269340]"
/experiment="EXISTENCE:mutant phenotype:GO:0032977
membrane insertase activity [PMID:25043001]"
/note="Subunit of the GET complex; involved in insertion
of tail-anchored proteins proteins into the ER membrane;
required for the retrieval of HDEL proteins from the Golgi
to the ER in an ERD2 dependent fashion and for meiotic
nuclear division; Get1p and Get2p form an aqueous channel
for protein translocation and insertion into the membrane"
/codon_start=1
/product="GET complex subunit GET2"
/protein_id="NP_011006.2"
/db_xref="GeneID:856817"
/db_xref="SGD:S000000885"
/translation="MSELTEAEKRRLLRERRQKKFSNGGASSRLNKITGQASSHLNAE
SPLDAPSAAKTTPPASVHSATPDIKEDSNVAPQLDLLKQLAAMQGQGTGKSTPQDSST
PDLLSLLSSMNTGMPSAEGTPSFGQAAPAAPINQAALDYHDYLLNRLKAWTILVKWVF
FLLPYLYLITRPNSSVWPAYAFTQSAWFAPLRNPSNFTRIFATFEFLSISIYYQLLKN
VEHKSKIKNLQDTNKLVKLVSLVPEGVIPVANLKGKLITLLQYWDLLSMLITDISFVL
IVLGLLTYL"
gene <327065..>327451
/locus_tag="YER084W"
/db_xref="GeneID:856816"
mRNA <327065..>327451
/locus_tag="YER084W"
/product="uncharacterized protein"
/transcript_id="NM_001270744.1"
/db_xref="GeneID:856816"
CDS 327065..327451
/locus_tag="YER084W"
/note="hypothetical protein; expressed at both mRNA and
protein levels"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001257673.1"
/db_xref="GeneID:856816"
/db_xref="SGD:S000000886"
/translation="MEELICTYPYHSNLFMFLFLFFCPSKRARRGHPKFLFTLCYKSN
HLIPKLLPPSLFTKRVMLNPSSHPPSPDFPTGSSASPRVKLRPSTLWAPPLTVSSDFA
ASSSSTAPVTVTDKPVTPAVSKRYQP"
gene complement(<327619..>328140)
/gene="LDM1"
/locus_tag="YER085C"
/db_xref="GeneID:856818"
mRNA complement(<327619..>328140)
/gene="LDM1"
/locus_tag="YER085C"
/product="myosin adaptor LDM1"
/transcript_id="NM_001178976.3"
/db_xref="GeneID:856818"
CDS complement(327619..328140)
/gene="LDM1"
/locus_tag="YER085C"
/note="Myosin adaptor; binds globular tail domain of Myo2p
and lipid droplet (LD) surface protein Ldo16p to enable
actin-dependent LD motility; has additional roles in LD
contact sites to vacuole and endoplasmic reticulum,
suggesting a coordination of LD motility and organelle
tethering; second role in mitochondrial transport"
/codon_start=1
/product="myosin adaptor LDM1"
/protein_id="NP_011008.3"
/db_xref="GeneID:856818"
/db_xref="SGD:S000000887"
/translation="MNFKEPLVNFLKCVLNNINSAFSHRIDQLQLQLLRETNILRVLN
RGIERLFSENPRTHSVSRIVVEKDVGCRIGRYVEGSKYELVESRAKEIQIYYERMVFE
ITQELKGDKRVFALIANLSQRYSAERKEIRNVKVHGGRPCNENEFQVIPIRFKPINLE
RRARLIRKKKTIN"
gene <328477..>330207
/gene="ILV1"
/locus_tag="YER086W"
/gene_synonym="ISO1"
/db_xref="GeneID:856819"
mRNA <328477..>330207
/gene="ILV1"
/locus_tag="YER086W"
/gene_synonym="ISO1"
/product="threonine ammonia-lyase ILV1"
/transcript_id="NM_001178977.1"
/db_xref="GeneID:856819"
CDS 328477..330207
/gene="ILV1"
/locus_tag="YER086W"
/gene_synonym="ISO1"
/EC_number="4.3.1.19"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:24769239|PMID:14562095|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:genetic interaction:GO:0006567
L-threonine catabolic process [PMID:7042346]"
/experiment="EXISTENCE:mutant phenotype:GO:0004794
threonine deaminase activity [PMID:5345980]"
/experiment="EXISTENCE:mutant phenotype:GO:0009097
isoleucine biosynthetic process [PMID:5345980]"
/note="Threonine deaminase, catalyzes first step in
isoleucine biosynthesis; expression is under general amino
acid control; ILV1 locus exhibits highly positioned
nucleosomes whose organization is independent of known
ILV1 regulation"
/codon_start=1
/product="threonine ammonia-lyase ILV1"
/protein_id="NP_011009.1"
/db_xref="GeneID:856819"
/db_xref="SGD:S000000888"
/translation="MSATLLKQPLCTVVRQGKQSKVSGLNLLRLKAHLHRQHLSPSLI
KLHSELKLDELQTDNTPDYVRLVLRSSVYDVINESPISQGVGLSSRLNTNVILKREDL
LPVFSFKLRGAYNMIAKLDDSQRNQGVIACSAGNHAQGVAFAAKHLKIPATIVMPVCT
PSIKYQNVSRLGSQVVLYGNDFDEAKAECAKLAEERGLTNIPPFDHPYVIAGQGTVAM
EILRQVRTANKIGAVFVPVGGGGLIAGIGAYLKRVAPHIKIIGVETYDAATLHNSLQR
NQRTPLPVVGTFADGTSVRMIGEETFRVAQQVVDEVVLVNTDEICAAVKDIFEDTRSI
VEPSGALSVAGMKKYISTVHPEIDHTKNTYVPILSGANMNFDRLRFVSERAVLGEGKE
VFMLVTLPDVPGAFKKMQKIIHPRSVTEFSYRYNEHRHESSSEVPKAYIYTSFSVVDR
EKEIKQVMQQLNALGFEAVDISDNELAKSHGRYLVGGASKVPNERIISFEFPERPGAL
TRFLGGLSDSWNLTLFHYRNHGADIGKVLAGISVPPRENLTFQKFLEDLGYTYHDETD
NTVYQKFLKY"
gene <330576..>332306
/gene="AIM10"
/locus_tag="YER087W"
/db_xref="GeneID:856820"
mRNA <330576..>332306
/gene="AIM10"
/locus_tag="YER087W"
/product="putative proline--tRNA ligase AIM10"
/transcript_id="NM_001178978.1"
/db_xref="GeneID:856820"
CDS 330576..332306
/gene="AIM10"
/locus_tag="YER087W"
/EC_number="6.1.1.15"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/note="Protein with similarity to tRNA synthetases;
non-tagged protein is detected in purified mitochondria;
null mutant is viable and displays elevated frequency of
mitochondrial genome loss"
/codon_start=1
/product="putative proline--tRNA ligase AIM10"
/protein_id="NP_011010.1"
/db_xref="GeneID:856820"
/db_xref="SGD:S000000889"
/translation="MLKYRTLSRSCHIFHPKSLSNNTLKSETTQELLQTVGFVRRSQV
GLFQWLPLGLRSLNKVSNAIRNRMDSDGGAIEVSLSAISSKALWQATDRWNNSELFKL
KDSKGKQYCLTATCEEDITDLMKNYIASYKDMPITIYQMTRKYRDEIRPRGGILRGRE
FLMKDAYSFASNEEDAFASFQKLDDTYNKIFKDLKIPFVSAWADSGDIGGEFSKEFHL
IHESGEDTLMSCKHCGDISTLDMSQSYPEKDGQYSGDVDCKYALTKDHSTLICFYYPK
DRQLNWNLALNAMDKDIDLTLRNKPNDHVLQVYEKDNEDIMFSKILRVMDCRLNSKSN
FPDFPLKKYLKNNFGQISDVSIVDAQENEICGKCEEGRLEPLKSIEVGHIFLLGNKYS
KPLNVKFVDKENKNETFVHMGCYGIGVSRLVGAIAELGRDSNGFRWPAIMAPYKVSIC
TGPNNPENSQRLQDVKSELLNDPTMQNLQNDILDQFNEKLGIGARIKLSHAMGIPLCV
IVGSKSWPNVEIEVRGIRWGEKDLWRKQFEKRCSELQWKCTKNEHGIEKHTVPIQHLA
EVIGVLLKDM"
gene complement(<332582..>332830)
/gene="SBH1"
/locus_tag="YER087C-B"
/gene_synonym="SEB1; YER087C-A"
/db_xref="GeneID:856821"
mRNA complement(<332582..>332830)
/gene="SBH1"
/locus_tag="YER087C-B"
/gene_synonym="SEB1; YER087C-A"
/product="Arf family guanine nucleotide exchange factor
SBH1"
/transcript_id="NM_001180032.3"
/db_xref="GeneID:856821"
CDS complement(332582..332830)
/gene="SBH1"
/locus_tag="YER087C-B"
/gene_synonym="SEB1; YER087C-A"
/experiment="EXISTENCE:direct assay:GO:0005085
guanyl-nucleotide exchange factor activity
[PMID:12750387]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762|PMID:27831485]"
/experiment="EXISTENCE:direct assay:GO:0005784 Sec61
translocon complex [PMID:8612571|PMID:7758110]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:31201345]"
/experiment="EXISTENCE:direct assay:GO:0008320 protein
transmembrane transporter activity [PMID:9252322]"
/experiment="EXISTENCE:direct assay:GO:0015450
protein-transporting ATPase activity [PMID:7758110]"
/experiment="EXISTENCE:direct assay:GO:0031204
post-translational protein targeting to membrane,
translocation [PMID:7758110]"
/experiment="EXISTENCE:mutant phenotype:GO:0006616
SRP-dependent cotranslational protein targeting to
membrane, translocation [PMID:19857245]"
/note="Beta subunit of Sec61p ER translocation complex
(Sec61p-Sss1p-Sbh1p); involved in protein translocation
into the endoplasmic reticulum; interacts with the exocyst
complex and also with Rtn1p; cotranslationally
N-acetylated by NatA; SBH1 has a paralog, SBH2, that arose
from the whole genome duplication"
/codon_start=1
/product="Arf family guanine nucleotide exchange factor
SBH1"
/protein_id="NP_011011.3"
/db_xref="GeneID:856821"
/db_xref="SGD:S000002128"
/translation="MSSPTPPGGQRTLQKRKQGSSQKVAASAPKKNTNSNNSILKIYS
DEATGLRVDPLVVLFLAVGFIFSVVALHVISKVAGKLF"
gene complement(<333176..>335188)
/gene="DOT6"
/locus_tag="YER088C"
/gene_synonym="PBF2"
/db_xref="GeneID:856822"
mRNA complement(<333176..>335188)
/gene="DOT6"
/locus_tag="YER088C"
/gene_synonym="PBF2"
/product="Dot6p"
/transcript_id="NM_001178979.3"
/db_xref="GeneID:856822"
CDS complement(333176..335188)
/gene="DOT6"
/locus_tag="YER088C"
/gene_synonym="PBF2"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:19158363]"
/experiment="EXISTENCE:direct assay:GO:0070210
Rpd3L-Expanded complex [PMID:19040720]"
/experiment="EXISTENCE:mutant phenotype:GO:0000183 rDNA
heterochromatin formation [PMID:9755194]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:19158363]"
/experiment="EXISTENCE:mutant phenotype:GO:0031509
subtelomeric heterochromatin formation [PMID:9755194]"
/note="Protein involved in rRNA and ribosome biogenesis;
activated in stochastic pulses of nuclear localization;
binds polymerase A and C motif; subunit of the RPD3L
histone deacetylase complex; has chromatin specific SANT
domain; involved in telomeric gene silencing and
filamentation; relative distribution to the nucleus
increases upon DNA replication stress"
/codon_start=1
/product="Dot6p"
/protein_id="NP_011012.3"
/db_xref="GeneID:856822"
/db_xref="SGD:S000000890"
/translation="MSISTSLNSASIHLSSMDTHPQLHSLTRQPHSSSTAMSKNEAQE
SSPSLPASSSSSTSASASASSKNSSKNPSSWDPQDDLLLRHLKEVKKMGWKDISQYFP
NRTPNACQFRWRRLKSGNLKSNKTALIDINTYTGPLKITHGDETANAQQKPSKKVEEN
VLTEDTAEFTTTSSIPIPSRKTSLPSFHASMSFSQSPSNVTPTTIVSNAASSMPFAPP
TLPAALPHHPHQHLHHHPHHKTLKPRSNSHSFTNSLNQDPIVRSNDEEKYGFIPKVFV
RSRRSSFAYPQQVAITTTPSSPNSSHVLLSSKSRRGSLANWSRRSSFNVSSNNTSRRS
SMILAPNSVSNIFNVNNSGSNTASTSNTNSRRESVIKKEFQQRLNNLSNSGGPTSNNG
PIFPNSYTFMDLPHSSSVSSSSTLHKSKRGSFSGHSMKSSCNPTNLWSKDEDALLMEN
KKRNLSVMELSILLPQRTEVEIQWRLNALSSDADMLSPTHSPQKTLSKKTCPRMFKSG
STTDDDKGSDKEDVMGDGSNDDDEDNVDPLHRAKQSSNKTVFSSSSSNISSKDVSPDP
IFSPDPADDSSNTSDAGSRCTITSDTSSSAATMNRTPNSKNPQDIALLNNFRSEAITP
RPKPSSTTTSITTETTNNMINHSSSTTTTTNNSPLPSINTIFKDML"
gene complement(<335946..>337340)
/gene="PTC2"
/locus_tag="YER089C"
/db_xref="GeneID:856823"
mRNA complement(<335946..>337340)
/gene="PTC2"
/locus_tag="YER089C"
/product="type 2C protein phosphatase PTC2"
/transcript_id="NM_001178980.1"
/db_xref="GeneID:856823"
CDS complement(335946..337340)
/gene="PTC2"
/locus_tag="YER089C"
/EC_number="3.1.3.16"
/experiment="EXISTENCE:direct assay:GO:0004722 protein
serine/threonine phosphatase activity
[PMID:9528768|PMID:10580002]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12477803|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:12477803]"
/experiment="EXISTENCE:direct assay:GO:1990439 MAP kinase
serine/threonine phosphatase activity [PMID:12477803]"
/experiment="EXISTENCE:mutant phenotype:GO:1900102
negative regulation of endoplasmic reticulum unfolded
protein response [PMID:9528768]"
/experiment="EXISTENCE:physical interaction:GO:1900102
negative regulation of endoplasmic reticulum unfolded
protein response [PMID:9528768]"
/note="Type 2C protein phosphatase (PP2C);
dephosphorylates Hog1p to limit maximal osmostress induced
kinase activity; dephosphorylates Ire1p to downregulate
the unfolded protein response; dephosphorylates Cdc28p;
inactivates the DNA damage checkpoint; PTC2 has a paralog,
PTC3, that arose from the whole genome duplication"
/codon_start=1
/product="type 2C protein phosphatase PTC2"
/protein_id="NP_011013.1"
/db_xref="GeneID:856823"
/db_xref="SGD:S000000891"
/translation="MGQILSNPVIDKESHSGADSLTAFGLCAMQGWRMSMEDSHILEP
NVLTKSDKDHIAFYGIFDGHGGAKVAEYCGNKIVEILQEQKSFHEGNLPRALIDTFIN
TDVKLLQDPVMKEDHSGCTATSILVSKSQNLLVCGNAGDSRTVLATDGNAKALSYDHK
PTLASEKSRIVAADGFVEMDRVNGNLALSRAIGDFEFKSNPKLGPEEQIVTCVPDILE
HSLDYDRDEFVILACDGIWDCLTSQDCVDLVHLGLREGKTLNEISSRIIDVCCAPTTE
GTGIGCDNMSIVVVALLKEGEDVAQWSDRMKSKAHRTSVRSFADKRRRVFSYYDFSKC
NDEQVFAITTKKPQDKFTRDHEAAVASVTAADNDDPMDIDDTDADTDAENLDPSSQSK
SKTSGPIDLASLEALLGATGGVKTDSNGNKVTYTLPQSALAQLLQTMGHDPASSHPEN
DSNTDHKAGRSHLQ"
gene <337949..>339472
/gene="TRP2"
/locus_tag="YER090W"
/db_xref="GeneID:856824"
mRNA <337949..>339472
/gene="TRP2"
/locus_tag="YER090W"
/product="anthranilate synthase TRP2"
/transcript_id="NM_001178981.1"
/db_xref="GeneID:856824"
CDS 337949..339472
/gene="TRP2"
/locus_tag="YER090W"
/EC_number="4.1.3.27"
/experiment="EXISTENCE:direct assay:GO:0004049
anthranilate synthase activity [PMID:3881257]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0004049
anthranilate synthase activity [PMID:6323449]"
/experiment="EXISTENCE:mutant phenotype:GO:0000162
L-tryptophan biosynthetic process [PMID:8432699]"
/experiment="EXISTENCE:physical interaction:GO:0005950
anthranilate synthase complex [PMID:3881257]"
/note="Anthranilate synthase; catalyzes the initial step
of tryptophan biosynthesis, forms multifunctional
hetero-oligomeric anthranilate synthase:indole-3-glycerol
phosphate synthase enzyme complex with Trp3p"
/codon_start=1
/product="anthranilate synthase TRP2"
/protein_id="NP_011014.1"
/db_xref="GeneID:856824"
/db_xref="SGD:S000000892"
/translation="MTASIKIQPDIDSLKQLQQQNDDSSINMYPVYAYLPSLDLTPHV
AYLKLAQLNNPDRKESFLLESAKTNNELDRYSFIGISPRKTIKTGPTEGIETDPLEIL
EKEMSTFKVAENVPGLPKLSGGAIGYISYDCVRYFEPKTRRPLKDVLRLPEAYLMLCD
TIIAFDNVFQRFQIIHNINTNETSLEEGYQAAAQIITDIVSKLTDDSSPIPYPEQPPI
KLNQTFESNVGKEGYENHVSTLKKHIKKGDIIQGVPSQRVARPTSLHPFNIYRHLRTV
NPSPYLFYIDCLDFQIIGASPELLCKSDSKNRVITHPIAGTVKRGATTEEDDALADQL
RGSLKDRAEHVMLVDLARNDINRICDPLTTSVDKLLTIQKFSHVQHLVSQVSGVLRPE
KTRFDAFRSIFPAGTVSGAPKVRAMELIAELEGERRGVYAGAVGHWSYDGKTMDNCIA
LRTMVYKDGIAYLQAGGGIVYDSDEYDEYVETMNKMMANHSTIVQAEELWADIVGSA"
gene complement(<339864..>342167)
/gene="MET6"
/locus_tag="YER091C"
/db_xref="GeneID:856825"
mRNA complement(<339864..>342167)
/gene="MET6"
/locus_tag="YER091C"
/product="5-methyltetrahydropteroyltriglutamate-
homocysteine S-methyltransferase"
/transcript_id="NM_001178982.3"
/db_xref="GeneID:856825"
CDS complement(339864..342167)
/gene="MET6"
/locus_tag="YER091C"
/EC_number="2.1.1.14"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836]"
/experiment="EXISTENCE:mutant phenotype:GO:0003871
5-methyltetrahydropteroyltriglutamate-homocysteine
S-methyltransferase activity [PMID:3542720]"
/experiment="EXISTENCE:mutant phenotype:GO:0009086
methionine biosynthetic process [PMID:1101032]"
/note="Cobalamin-independent methionine synthase; involved
in methionine biosynthesis and regeneration; requires a
minimum of two glutamates on the methyltetrahydrofolate
substrate, similar to bacterial metE homologs"
/codon_start=1
/product="5-methyltetrahydropteroyltriglutamate-
homocysteine S-methyltransferase"
/protein_id="NP_011015.3"
/db_xref="GeneID:856825"
/db_xref="SGD:S000000893"
/translation="MVQSAVLGFPRIGPNRELKKATEGYWNGKITVDELFKVGKDLRT
QNWKLQKEAGVDIIPSNDFSFYDQVLDLSLLFNVIPDRYTKYDLSPIDTLFAMGRGLQ
RKATETEKAVDVTALEMVKWFDSNYHYVRPTFSKTTQFKLNGQKPVDEFLEAKELGIH
TRPVLLGPVSYLFLGKADKDSLDLEPLSLLEQLLPLYTEILSKLASAGATEVQIDEPV
LVLDLPANAQAAIKKAYTYFGEQSNLPKITLATYFGTVVPNLDAIKGLPVAALHVDFV
RAPEQFDEVVAAIGNKQTLSVGIVDGRNIWKNDFKKSSAIVNKAIEKLGADRVVVATS
SSLLHTPVDLNNETKLDAEIKGFFSFATQKLDEVVVITKNVSGQDVAAALEANAKSVE
SRGKSKFIHDAAVKARVASIDEKMSTRAAPFEQRLPEQQKVFNLPLFPTTTIGSFPQT
KDIRINRNKFNKGTISAEEYEKFINSEIEKVIRFQEEIGLDVLVHGEPERNDMVQYFG
EQINGYAFTVNGWVQSYGSRYVRPPIIVGDLSRPKAMSVKESVYAQSITSKPVKGMLT
GPITCLRWSFPRDDVDQKTQAMQLALALRDEVNDLEAAGIKVIQVDEPALREGLPLRE
GTERSAYYTWAAEAFRVATSGVANKTQIHSHFCYSDLDPNHIKALDADVVSIEFSKKD
DANYIAEFKNYPNHIGLGLFDIHSPRIPSKDEFIAKISTILKSYPAEKFWVNPDCGLK
TRGWEETRLSLTHMVEAAKYFREQYKN"
gene <342855..>343232
/gene="IES5"
/locus_tag="YER092W"
/db_xref="GeneID:856827"
mRNA <342855..>343232
/gene="IES5"
/locus_tag="YER092W"
/product="Ies5p"
/transcript_id="NM_001178983.3"
/db_xref="GeneID:856827"
CDS 342855..343232
/gene="IES5"
/locus_tag="YER092W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0000722
telomere maintenance via recombination [PMID:23390378]"
/experiment="EXISTENCE:physical interaction:GO:0031011
Ino80 complex [PMID:24034245]"
/note="Non-essential INO80 chromatin remodeling complex
subunit; deletion affects telomere maintenance via
recombination"
/codon_start=1
/product="Ies5p"
/protein_id="NP_011017.3"
/db_xref="GeneID:856827"
/db_xref="SGD:S000000894"
/translation="MPSKDPESVIDKEIRKISARNDELIKQDGTLKREYTTLLRKVSS
VITVLNSIDDADTGSAETELPRLISQATVEKVPELKWYNDQISLITEKLEDDEDIEVP
EELMDAYTLYKETPLLYNDTHTP"
gene complement(<343320..>347612)
/gene="TSC11"
/locus_tag="YER093C"
/gene_synonym="AVO3"
/db_xref="GeneID:856828"
mRNA complement(<343320..>347612)
/gene="TSC11"
/locus_tag="YER093C"
/gene_synonym="AVO3"
/product="TORC2 complex subunit TSC11"
/transcript_id="NM_001178984.1"
/db_xref="GeneID:856828"
CDS complement(343320..347612)
/gene="TSC11"
/locus_tag="YER093C"
/gene_synonym="AVO3"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:30969890]"
/experiment="EXISTENCE:genetic interaction:GO:0031505
fungal-type cell wall organization [PMID:15809876]"
/experiment="EXISTENCE:mutant phenotype:GO:0030148
sphingolipid biosynthetic process [PMID:9804843]"
/experiment="EXISTENCE:mutant phenotype:GO:0030950
establishment or maintenance of actin cytoskeleton
polarity [PMID:15809876]"
/experiment="EXISTENCE:mutant phenotype:GO:0031505
fungal-type cell wall organization [PMID:15809876]"
/experiment="EXISTENCE:mutant phenotype:GO:0043495
protein-membrane adaptor activity [PMID:30969890]"
/experiment="EXISTENCE:mutant phenotype:GO:0060090
molecular adaptor activity [PMID:16002396]"
/experiment="EXISTENCE:mutant phenotype:GO:0072659 protein
localization to plasma membrane [PMID:30969890]"
/experiment="EXISTENCE:physical interaction:GO:0001558
regulation of cell growth [PMID:12408816]"
/experiment="EXISTENCE:physical interaction:GO:0030950
establishment or maintenance of actin cytoskeleton
polarity [PMID:12408816]"
/experiment="EXISTENCE:physical interaction:GO:0031932
TORC2 complex [PMID:12408816]"
/note="Subunit of TORC2, a regulator of plasma membrane
(PM) homeostasis; TORC2 also regulates actin cytoskeletal
dynamics during polarized growth and cell wall integrity;
contains an N-terminal armadillo repeat region necessary
for recruitment to the PM and for anchoring Tor2p and
Avo1p at the PM; acts along with Avo1p as a scaffold
important for integrity of the TORC2 complex; involved in
sphingolipid metabolism; contains a RasGEFN domain"
/codon_start=1
/product="TORC2 complex subunit TSC11"
/protein_id="NP_011018.1"
/db_xref="GeneID:856828"
/db_xref="SGD:S000000895"
/translation="MSIPHSAKQSSPLSSRRRSVTNTTPLLTPRHSRDNSSTQISSAK
NITSSSPSTITNESSKRNKQNLVLSTSFISTKRLENSAPSPTSPLMARRTRSTMTKAL
LNLKAEINNQYQELARLRKKKDDIEHLRDSTISDIYSGSYSTNHLQKHSMRIRANTQL
REIDNSIKRVEKHIFDLKQQFDKKRQRSLTTSSSIKADVGSIRNDDGQNNDSEELGDH
DSLTDQVTLDDEYLTTPTSGTERNSQQNLNRNSTVNSRNNENHSTLSIPDLDGSNKVN
LTGDTEKDLGDLENENQIFTSTTTEAATWLVSDYMQSFQEKNVNPDFIAQKANGLVTL
LKEHSEIRKDLVLTSFMSSIQNLLLNGNKLIAASAYRVCRYLINSSIFIDELLELRLD
AFIIISLAKDNSFQIEREQALKMVRRFIEYNNGVTQGIMQAIISCVEKPEDSLRHMAL
ETLLELCFVAPEMVKECRGMRVIEGFLQDYTSFSLASVILDTILQLMATHKTRQHFLE
DFNVSVLTTVFSDTNTKSNVNVEKMQNASTLISITLNSYNGFMLFSNNNFKPLKQLVS
FFQIPICAQYLIDIFLDVLKIKPLPYKPRGRHSHSFKPIPSQYYKECMSVNQRLALIV
LILENSEFVPHLLELLNEEDRDDHLVAKGRYLLTEYFNLRMNLVDKKYTSVSKPIYKE
NFTYVNETFQFKKIAYKMNRNRNTIGMSGIDYAQNIKSFSKNIKENTLLREVDDFRFR
RMVYDSKVLQTKDFTRWNWNIINELLEGPLLNKKQLEELVKSTKFIRRLLVFYRPLRL
RFSNVNKGAKLSQKYVQVGCQFFKTLTATPEGMKILMDDTKIIPQLASLMFRAMEGNI
SGNIFNKNKLREKIIFGYFKFIGILTQSKNGVHILTRWNFFTVIYKMFQFESKLGLEF
LLLTIPELDLKYSSHCRVIIGKALVVANEKVRIEATKHIGDKLKELLSTKESDLKLKA
NKVKLQQFKMEMLTRQLYDLSPSVVAVADQALYECIVAGNGSEELGTSFRMFLNQMVF
IRSPILFELLSRPYGFQLLNEINFVKEERDSWLSKKNIEYVHIVEEFLKKNESINAKS
LTFQQKSRLPLHFYESLTKTEDGILLLSQTGDLVTFMNVIKKYVNGNNMATVENAKEI
LDLKAALWCVGFIGSTELGIGLLDNYSLVEDIIEVAYNASVTSVRFTAFYVLGLISMT
REGCEILDEMGWNCCVSVQDEPIGIALPNRLDRFLSYNEHKWSAFGEYSDEMIVFNKS
DGDLIEKCLPIEFDLDKLLKEKDTAENPLNEKIITNKYDNDITSQTITVSGENSSLFA
NEGLSSPYVTQYRNDDDSIESKVLHIVSQLGNHILSNHAVKEITEINNKYGPRLFENE
KMFFKVFNMMSKYRFKPHVRKFLCGLFINNRALENVIRHDNKRDKRPANFTR"
gene complement(<347912..>348400)
/gene="AIM11"
/locus_tag="YER093C-A"
/gene_synonym="GEP8"
/db_xref="GeneID:856829"
mRNA complement(join(<347912..348201,348277..>348400))
/gene="AIM11"
/locus_tag="YER093C-A"
/gene_synonym="GEP8"
/product="Aim11p"
/transcript_id="NM_001180860.1"
/db_xref="GeneID:856829"
CDS complement(join(347912..348201,348277..348400))
/gene="AIM11"
/locus_tag="YER093C-A"
/gene_synonym="GEP8"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:35563734]"
/note="hypothetical protein; null mutant is viable but
shows increased loss of mitochondrial genome and synthetic
interaction with prohibitin (phb1); contains an intron;
SWAT-GFP and mCherry fusion proteins localize to the
mitochondria; localizes to the peroxisome in glucose;
YER093C-A has a paralog, YBL059W, that arose from the
whole genome duplication"
/codon_start=1
/product="Aim11p"
/protein_id="NP_011019.1"
/db_xref="GeneID:856829"
/db_xref="SGD:S000002960"
/translation="MIEEKKELKKRRVLQMARFYGAAAFTLITMRLISRAIKVRKYVP
SIFQQNYKLPPFSQRNEAMSALTYASAASIGTFSTLIFGFCWALDISTAREFVFKTRE
FMSLPQALETDTSMDEETSKLTKQLQDLLSSENNK"
gene complement(<348729..>349346)
/gene="PUP3"
/locus_tag="YER094C"
/gene_synonym="SCS32"
/db_xref="GeneID:856830"
mRNA complement(<348729..>349346)
/gene="PUP3"
/locus_tag="YER094C"
/gene_synonym="SCS32"
/product="proteasome core particle subunit beta 3"
/transcript_id="NM_001178985.3"
/db_xref="GeneID:856830"
CDS complement(348729..349346)
/gene="PUP3"
/locus_tag="YER094C"
/gene_synonym="SCS32"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0010499 proteasomal
ubiquitin-independent protein catabolic process
[PMID:19162040]"
/experiment="EXISTENCE:direct assay:GO:0019774 proteasome
core complex, beta-subunit complex [PMID:9087403]"
/experiment="EXISTENCE:direct assay:GO:0043161
proteasome-mediated ubiquitin-dependent protein catabolic
process [PMID:11545745|PMID:19029916]"
/experiment="EXISTENCE:genetic interaction:GO:0061133
endopeptidase activator activity [PMID:9207060]"
/note="Beta 3 subunit of the 20S proteasome; involved in
ubiquitin-dependent catabolism; human homolog is subunit
C10"
/codon_start=1
/product="proteasome core particle subunit beta 3"
/protein_id="NP_011020.3"
/db_xref="GeneID:856830"
/db_xref="SGD:S000000896"
/translation="MSDPSSINGGIVVAMTGKDCVAIACDLRLGSQSLGVSNKFEKIF
HYGHVFLGITGLATDVTTLNEMFRYKTNLYKLKEERAIEPETFTQLVSSSLYERRFGP
YFVGPVVAGINSKSGKPFIAGFDLIGCIDEAKDFIVSGTASDQLFGMCESLYEPNLEP
EDLFETISQALLNAADRDALSGWGAVVYIIKKDEVVKRYLKMRQD"
gene <349980..>351182
/gene="RAD51"
/locus_tag="YER095W"
/gene_synonym="MUT5"
/db_xref="GeneID:856831"
mRNA <349980..>351182
/gene="RAD51"
/locus_tag="YER095W"
/gene_synonym="MUT5"
/product="recombinase RAD51"
/transcript_id="NM_001178986.3"
/db_xref="GeneID:856831"
CDS 349980..351182
/gene="RAD51"
/locus_tag="YER095W"
/gene_synonym="MUT5"
/experiment="EXISTENCE:direct assay:GO:0000086 G2/M
transition of mitotic cell cycle [PMID:32083180]"
/experiment="EXISTENCE:direct assay:GO:0000150 DNA strand
exchange activity [PMID:8066464]"
/experiment="EXISTENCE:direct assay:GO:0000228 nuclear
chromosome [PMID:11459983]"
/experiment="EXISTENCE:direct assay:GO:0000262
mitochondrial chromosome [PMID:26540255]"
/experiment="EXISTENCE:direct assay:GO:0000794 condensed
nuclear chromosome [PMID:9427283]"
/experiment="EXISTENCE:direct assay:GO:0003677 DNA binding
[PMID:26540255]"
/experiment="EXISTENCE:direct assay:GO:0003690
double-stranded DNA binding [PMID:32083180]"
/experiment="EXISTENCE:direct assay:GO:0003697
single-stranded DNA binding [PMID:20371520|PMID:32083180]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:26540255]"
/experiment="EXISTENCE:direct assay:GO:0006310 DNA
recombination [PMID:22955832]"
/experiment="EXISTENCE:direct assay:GO:0008094
ATP-dependent activity, acting on DNA [PMID:8066464]"
/experiment="EXISTENCE:direct assay:GO:0030491
heteroduplex formation [PMID:10506208]"
/experiment="EXISTENCE:direct assay:GO:0042148 DNA strand
invasion [PMID:8066464|PMID:9271413]"
/experiment="EXISTENCE:mutant phenotype:GO:0000709 meiotic
joint molecule formation [PMID:1581961]"
/experiment="EXISTENCE:mutant phenotype:GO:0000722
telomere maintenance via recombination [PMID:25822194]"
/experiment="EXISTENCE:mutant phenotype:GO:0003690
double-stranded DNA binding [PMID:32083180]"
/experiment="EXISTENCE:mutant phenotype:GO:0006289
nucleotide-excision repair [PMID:7026973]"
/experiment="EXISTENCE:mutant phenotype:GO:0006302
double-strand break repair [PMID:7026973]"
/experiment="EXISTENCE:mutant phenotype:GO:0007131
reciprocal meiotic recombination [PMID:16581767]"
/experiment="EXISTENCE:mutant phenotype:GO:0043504
mitochondrial DNA repair [PMID:26540255]"
/note="Strand exchange protein; forms helical filament
with DNA that searches for homology; involved in
recombinational repair of DNA DSBs during vegetative
growth and meiosis; phosphorylation by Cdc28p in G2/M
phase promotes DNA binding, strand invasion, and primer
extension; L1 and L2 motifs of DNA binding site I have
critical roles in ensuring DNA fidelity during meiotic
recombination; RAD51 and RAD4 pathways confer resistance
to benzo[a]pyrene dihydrodiol; homolog of Dmc1p and
bacterial RecA"
/codon_start=1
/product="recombinase RAD51"
/protein_id="NP_011021.3"
/db_xref="GeneID:856831"
/db_xref="SGD:S000000897"
/translation="MSQVQEQHISESQLQYGNGSLMSTVPADLSQSVVDGNGNGSSED
IEATNGSGDGGGLQEQAEAQGEMEDEAYDEAALGSFVPIEKLQVNGITMADVKKLRES
GLHTAEAVAYAPRKDLLEIKGISEAKADKLLNEAARLVPMGFVTAADFHMRRSELICL
TTGSKNLDTLLGGGVETGSITELFGEFRTGKSQLCHTLAVTCQIPLDIGGGEGKCLYI
DTEGTFRPVRLVSIAQRFGLDPDDALNNVAYARAYNADHQLRLLDAAAQMMSESRFSL
IVVDSVMALYRTDFSGRGELSARQMHLAKFMRALQRLADQFGVAVVVTNQVVAQVDGG
MAFNPDPKKPIGGNIMAHSSTTRLGFKKGKGCQRLCKVVDSPCLPEAECVFAIYEDGV
GDPREEDE"
gene <351698..>353236
/gene="SHC1"
/locus_tag="YER096W"
/db_xref="GeneID:856832"
mRNA <351698..>353236
/gene="SHC1"
/locus_tag="YER096W"
/product="Shc1p"
/transcript_id="NM_001178987.1"
/db_xref="GeneID:856832"
CDS 351698..353236
/gene="SHC1"
/locus_tag="YER096W"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:15755916]"
/experiment="EXISTENCE:direct assay:GO:0030437 ascospore
formation [PMID:11918806]"
/experiment="EXISTENCE:genetic interaction:GO:0006031
chitin biosynthetic process [PMID:11918806]"
/experiment="EXISTENCE:genetic interaction:GO:0008047
enzyme activator activity [PMID:11918806]"
/note="Sporulation-specific activator of Chs3p (chitin
synthase III); required for the synthesis of the chitosan
layer of ascospores; transcriptionally induced at alkaline
pH; SHC1 has a paralog, SKT5, that arose from the whole
genome duplication"
/codon_start=1
/product="Shc1p"
/protein_id="NP_011022.1"
/db_xref="GeneID:856832"
/db_xref="SGD:S000000898"
/translation="MSMTICSNTPGAYPEIGAYNEVDKQLESSGFSSDSSLILNKPEV
RQYWSSVSSHISRSGDVFTNDKEKISSSIGEDAMDIDASPSLIEKYNSFPTRKILPEQ
DEFENDVEDDASSSLKEKSQGSCEIEIASEISSEILNGTSADGNSEFHDFAEPPPSQN
ESVALSFSQSNDLDFLNNPSGSGSSNDINRSTSSISLPRHVSLDFNVYNSLCLTNEVT
ASESHNVAKFHLGKKNKKSLLPRWKTIEMYGEVVKKTQDIYSNFQYAQYILRVGLDTE
KLHELVKELEDESNSFSVDSLKEYLVNDAKVILKKLSAVGYPDAQYLLGDAYSSGVFG
KIKNRRAFLLFSAAAKRMHIESVYRTAICYECGLGVTRNAPKAVNFLTFAATKNHPAA
MYKLGVYSYHGLMGLPDDILTKMDGYRWLRRATSMASSFVCGAPFELANIYMTGYKDL
IISDPDYAMALYEKAAALGHTESARILEDARRSGGFVSRGHPPSAQKYHKTSHEAVAA
KKLI"
repeat_region 353399..353735
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006915"
rep_origin 353578..353636
/note="ARS516; Autonomously Replicating Sequence"
/db_xref="SGD:S000077388"
repeat_region 354136..354850
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006916"
repeat_region complement(354360..354730)
/note="Ty4 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006908"
repeat_region complement(354851..354917)
/note="Ty3 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006905"
gene 354934..355005
/locus_tag="YNCE0018W"
/db_xref="GeneID:856833"
tRNA 354934..355005
/locus_tag="YNCE0018W"
/product="tRNA-Glu"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE
analysis; thiolation of uridine at wobble position (34)
requires Ncs6p"
/db_xref="GeneID:856833"
/db_xref="SGD:S000006551"
gene <355466..>357730
/gene="UBP9"
/locus_tag="YER098W"
/db_xref="GeneID:856835"
mRNA <355466..>357730
/gene="UBP9"
/locus_tag="YER098W"
/product="putative ubiquitin-specific protease UBP9"
/transcript_id="NM_001178989.1"
/db_xref="GeneID:856835"
CDS 355466..357730
/gene="UBP9"
/locus_tag="YER098W"
/EC_number="3.4.19.12"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="Putative ubiquitin-specific protease that cleaves
ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that
arose from the whole genome duplication"
/codon_start=1
/product="putative ubiquitin-specific protease UBP9"
/protein_id="NP_011024.1"
/db_xref="GeneID:856835"
/db_xref="SGD:S000000900"
/translation="MIKRWLSVNRKKSHPEKNTQGNDEINRKATSLKKTKGSGDPSIA
KSPSAKSSTSSIPSNLASHERRSKFSSQTDNLAGNKHYHEHYHNMASTSDEREYDSST
TYEDRAFDTESSILFTTITDLMPYGDGSNKVFGYENFGNTCYCNSVLQCLYNIPEFRC
NVLRYPERVAAVNRIRKSDLKGSKIRVFTNESFETSTNSGNSNTGYQSNDNEDAHNHH
HLQQSDQDNSSSSTQEKQNNFERKRNSFMGFGKDKSNYKDSAKKDDNNEMERPQPVHT
VVMASDTLTEKLHEGCKKIIVGRPLLKQSDSLSKASTTDCQANSHCQCDSQGSRITSV
DDDVLVNPESCNDAVNNSNNNKENTFPTSEQRKKAALIRGPVLNVDHLLYPTEEATLY
NGLKDIFESITENLSLTGIVSPTEFVKILKKENVLFNTMMQQDAHEFLNFLLNDFSEY
IQRNNPRMRFGPQKTDNSNDNFITDLFKGTLTNRIKCLTCDNITSRDEPFLDFPIEVQ
GDEETDIQKMLKSYHQREMLNGVNKFYCNKCYGLQEAERMVGLKQLPHILSLHLKRFK
YSEEQKSNIKLFNKILYPLTLDVSSTFNTSVYKKYELSGVVIHMGSGPQHGHYVCICR
NEKFGWLLYDDETVESIKEETVLQFTGHPGDQTTAYVLFYKETQADKTENQNENIDTS
SQDQMQTDNNIEQLIKCDDWLRDRKLRAAANIERKKTLGNIPEVKTAETKTPLNDKKR
NKQKRKSRILSFIK"
gene complement(<358105..>359061)
/gene="PRS2"
/locus_tag="YER099C"
/db_xref="GeneID:856836"
mRNA complement(<358105..>359061)
/gene="PRS2"
/locus_tag="YER099C"
/product="ribose phosphate diphosphokinase subunit PRS2"
/transcript_id="NM_001178990.3"
/db_xref="GeneID:856836"
CDS complement(358105..359061)
/gene="PRS2"
/locus_tag="YER099C"
/EC_number="2.7.6.1"
/experiment="EXISTENCE:direct assay:GO:0002189 ribose
phosphate diphosphokinase complex [PMID:15280369]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0004749
ribose phosphate diphosphokinase activity [PMID:15280369]"
/experiment="EXISTENCE:genetic interaction:GO:0006015
5-phosphoribose 1-diphosphate biosynthetic process
[PMID:15280369]"
/experiment="EXISTENCE:mutant phenotype:GO:0006015
5-phosphoribose 1-diphosphate biosynthetic process
[PMID:10212224]"
/note="5-phospho-ribosyl-1(alpha)-pyrophosphate
synthetase; synthesizes PRPP, which is required for
nucleotide, histidine, and tryptophan biosynthesis; one of
five related enzymes, which are active as heteromultimeric
complexes; PRS2 has a paralog, PRS4, that arose from the
whole genome duplication"
/codon_start=1
/product="ribose phosphate diphosphokinase subunit PRS2"
/protein_id="NP_011025.3"
/db_xref="GeneID:856836"
/db_xref="SGD:S000000901"
/translation="MSTNSIKLLAGNSHPGLAELISQRLGVPLSKVGVYQYSNKETSV
TIGESIRDEDVYIIQTGYGEHEINDFLMELLILIHACKTASVRRITAVIPNFPYARQD
KKDKSRAPITAKLIANLLETAGCDHVITMDLHASQIQGFFHIPVDNLYGEPSVLNYIR
TKTDFNNAILVSPDAGGAKRVASLADKLDMNFALIHKERQKANEVSRMLLVGDVAGKS
CLLIDDMADTCGTLVKACDTLMDHGAKEVIAIVTHGIFSGSAREKLINSRLSRIVCTN
TVPVDLDLDIVDQVDISPTIAEAIRRLHNGESVSYLFTHAPV"
gene <359562..>360314
/gene="UBC6"
/locus_tag="YER100W"
/gene_synonym="DOA2"
/db_xref="GeneID:856837"
mRNA <359562..>360314
/gene="UBC6"
/locus_tag="YER100W"
/gene_synonym="DOA2"
/product="E2 ubiquitin-conjugating protein UBC6"
/transcript_id="NM_001178991.3"
/db_xref="GeneID:856837"
CDS 359562..360314
/gene="UBC6"
/locus_tag="YER100W"
/gene_synonym="DOA2"
/EC_number="2.3.2.23"
/experiment="EXISTENCE:direct assay:GO:0004842
ubiquitin-protein transferase activity [PMID:8396728]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:8396728]"
/experiment="EXISTENCE:direct assay:GO:0006513 protein
monoubiquitination [PMID:8396728]"
/experiment="EXISTENCE:genetic interaction:GO:0036503 ERAD
pathway [PMID:8396728|PMID:8641272|PMID:8781238]"
/experiment="EXISTENCE:mutant phenotype:GO:0000209 protein
polyubiquitination [PMID:19345192]"
/experiment="EXISTENCE:mutant phenotype:GO:0004842
ubiquitin-protein transferase activity
[PMID:8396728|PMID:18191224]"
/experiment="EXISTENCE:mutant phenotype:GO:0006513 protein
monoubiquitination [PMID:8396728]"
/experiment="EXISTENCE:mutant phenotype:GO:0036503 ERAD
pathway
[PMID:8781238|PMID:8393731|PMID:18191224|PMID:8641272]"
/note="Ubiquitin-conjugating enzyme involved in ERAD;
located at the cytosolic side of the ER membrane; tail
region contains a transmembrane segment at the C-terminus;
substrate of the ubiquitin-proteasome pathway;
ER-associated protein degradation is also known as ERAD"
/codon_start=1
/product="E2 ubiquitin-conjugating protein UBC6"
/protein_id="NP_011026.3"
/db_xref="GeneID:856837"
/db_xref="SGD:S000000902"
/translation="MATKQAHKRLTKEYKLMVENPPPYILARPNEDNILEWHYIITGP
ADTPYKGGQYHGTLTFPSDYPYKPPAIRMITPNGRFKPNTRLCLSMSDYHPDTWNPGW
SVSTILNGLLSFMTSDEATTGSITTSDHQKKTLARNSISYNTFQNVRFKLIFPEVVQE
NVETLEKRKLDEGDAANTGDETEDPFTKAAKEKVISLEEILDPEDRIRAEQALRQSEN
NSKKDGKEPNDSSSMVYIGIAIFLFLVGLFMK"
gene complement(<360502..>361794)
/gene="AST2"
/locus_tag="YER101C"
/db_xref="GeneID:856838"
mRNA complement(<360502..>361794)
/gene="AST2"
/locus_tag="YER101C"
/product="Ast2p"
/transcript_id="NM_001178992.1"
/db_xref="GeneID:856838"
CDS complement(360502..361794)
/gene="AST2"
/locus_tag="YER101C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276|PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0006612
protein targeting to membrane [PMID:7822420]"
/note="Lipid raft associated protein; overexpression
restores Pma1p localization to lipid rafts which is
required for targeting of Pma1p to the plasma membrane;
sometimes classified in the medium-chain
dehydrogenase/reductases (MDRs) superfamily; AST2 has a
paralog, AST1, that arose from the whole genome
duplication"
/codon_start=1
/product="Ast2p"
/protein_id="NP_011027.1"
/db_xref="GeneID:856838"
/db_xref="SGD:S000000903"
/translation="MAEKILENKDPKLEAMTVDHEVSAPKPIPVDEPTLTRVARPLRH
VRHIPVKSLVFHSKHGPITFSYENKIKLPISKNKLVVQVNYVGLNPVDMKIRNGYTKP
IYGEAGIGREYSGVITHVGDNLTNRWNVGDDVYGIYYHPKLAIGALQSSLLIDPRVDP
ILMRPKNTLSPEKAAGSLFCLGTALNLLAQLKEKDQLNTESNVLINGGTSSVGMFAIQ
LLKRYYKVSKKLVVVTSGNGAAVLSEHFPDLKDEIIFINYLSCRGKSSKPLRRMLDTG
KVVDYDDFNTLKETEDYTQGKFNVVLDFIGGYDILSHSSSLIHAKGAYITTVGDYVGN
YKKDVFDSWDNPSANARKMFGSMLWSYDYSHFYFDPNIKIIPKKNDWIHECGKLLNEG
VVDCVVDKVYSWKNFKEAFSYMATQRAQGKLIMKVEGF"
gene <363100..>363702
/gene="RPS8B"
/locus_tag="YER102W"
/db_xref="GeneID:856839"
mRNA <363100..>363702
/gene="RPS8B"
/locus_tag="YER102W"
/product="40S ribosomal protein eS8 RPS8B"
/transcript_id="NM_001178993.1"
/db_xref="GeneID:856839"
CDS 363100..363702
/gene="RPS8B"
/locus_tag="YER102W"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:18782943]"
/experiment="EXISTENCE:direct assay:GO:0022627 cytosolic
small ribosomal subunit [PMID:18782943]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/experiment="EXISTENCE:genetic interaction:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16246728]"
/note="Protein component of the small (40S) ribosomal
subunit; homologous to mammalian ribosomal protein S8, no
bacterial homolog; RPS8B has a paralog, RPS8A, that arose
from the whole genome duplication"
/codon_start=1
/product="40S ribosomal protein eS8 RPS8B"
/protein_id="NP_011028.1"
/db_xref="GeneID:856839"
/db_xref="SGD:S000000904"
/translation="MGISRDSRHKRSATGAKRAQFRKKRKFELGRQPANTKIGAKRIH
SVRTRGGNKKYRALRIETGNFSWASEGISKKTRIAGVVYHPSNNELVRTNTLTKAAIV
QIDATPFRQWFEAHYGQTLGKKKNVKEEETVAKSKNAERKWAARAASAKIESSVESQF
SAGRLYACISSRPGQSGRCDGYILEGEELAFYLRRLTAKK"
gene <364589..>366517
/gene="SSA4"
/locus_tag="YER103W"
/gene_synonym="YG107"
/db_xref="GeneID:856840"
mRNA <364589..>366517
/gene="SSA4"
/locus_tag="YER103W"
/gene_synonym="YG107"
/product="Hsp70 family chaperone SSA4"
/transcript_id="NM_001178994.3"
/db_xref="GeneID:856840"
CDS 364589..366517
/gene="SSA4"
/locus_tag="YER103W"
/gene_synonym="YG107"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11279056]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11279056]"
/experiment="EXISTENCE:genetic interaction:GO:0006457
protein folding [PMID:9789005]"
/experiment="EXISTENCE:genetic interaction:GO:0051082
unfolded protein binding [PMID:9789005]"
/experiment="EXISTENCE:mutant phenotype:GO:0006616
SRP-dependent cotranslational protein targeting to
membrane, translocation [PMID:8754838]"
/note="Heat shock protein that is highly induced upon
stress; plays a role in SRP-dependent cotranslational
protein-membrane targeting and translocation; translocates
to the nucleus under Cd2+ stress to mediate tolerance by
regulating VHS1 expression; cytoplasmic protein that
concentrates in nuclei upon starvation; member of the
HSP70 family; SSA4 has a paralog, SSA3, that arose from
the whole genome duplication"
/codon_start=1
/product="Hsp70 family chaperone SSA4"
/protein_id="NP_011029.3"
/db_xref="GeneID:856840"
/db_xref="SGD:S000000905"
/translation="MSKAVGIDLGTTYSCVAHFANDRVEIIANDQGNRTTPSYVAFTD
TERLIGDAAKNQAAMNPHNTVFDAKRLIGRKFDDPEVTNDAKHYPFKVIDKGGKPVVQ
VEYKGETKTFTPEEISSMILTKMKETAENFLGTEVKDAVVTVPAYFNDSQRQATKDAG
TIAGLNVLRIINEPTAAAIAYGLDKKSQKEHNVLIFDLGGGTFDVSLLSIDEGVFEVK
ATAGDTHLGGEDFDSRLVNFLAEEFKRKNKKDLTTNQRSLRRLRTAAERAKRTLSSSA
QTSIEIDSLFEGIDFYTSITRARFEELCADLFRSTLEPVEKVLADSKLDKSQIDEIVL
VGGSTRIPKVQKLVSDFFNGKEPNRSINPDEAVAYGAAVQAAILTGDQSSTTQDLLLL
DVAPLSLGIETAGGIMTKLIPRNSTIPTKKSEVFSTYADNQPGVLIQVFEGERTRTKD
NNLLGKFELSGIPPAPRGVPQIEVTFDIDANGILNVSAVEKGTGKSNKITITNDKGRL
SKEDIDKMVAEAEKFKAEDEQEAQRVQAKNQLESYAFTLKNSVSENNFKEKVGEEDAR
KLEAAAQDAINWLDASQAASTEEYKERQKELEGVANPIMSKFYGAAGGAPGAGPVPGA
GAGPTGAPDNGPTVEEVD"
gene <366802..>367428
/gene="RTT105"
/locus_tag="YER104W"
/db_xref="GeneID:856841"
mRNA <366802..>367428
/gene="RTT105"
/locus_tag="YER104W"
/product="Rtt105p"
/transcript_id="NM_001178995.3"
/db_xref="GeneID:856841"
CDS 366802..367428
/gene="RTT105"
/locus_tag="YER104W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0060590 ATPase
regulator activity [PMID:39145931]"
/experiment="EXISTENCE:direct assay:GO:0140597 protein
carrier chaperone [PMID:30065069]"
/experiment="EXISTENCE:mutant phenotype:GO:0000723
telomere maintenance [PMID:33724421]"
/experiment="EXISTENCE:mutant phenotype:GO:0000724
double-strand break repair via homologous recombination
[PMID:34140406]"
/experiment="EXISTENCE:mutant phenotype:GO:0000727
double-strand break repair via break-induced replication
[PMID:34140406]"
/experiment="EXISTENCE:mutant phenotype:GO:0006606 protein
import into nucleus [PMID:30065069]"
/experiment="EXISTENCE:mutant phenotype:GO:0010526
transposable element silencing [PMID:11779788]"
/experiment="EXISTENCE:mutant phenotype:GO:0031507
heterochromatin formation [PMID:33724421]"
/note="Chaperone for Replication Protein A complex (RPA);
involved in nuclear import of RPA and its binding to ssDNA
at replication forks; has a role in regulation of Ty1
transposition"
/codon_start=1
/product="Rtt105p"
/protein_id="NP_011030.3"
/db_xref="GeneID:856841"
/db_xref="SGD:S000000906"
/translation="MYSNHNLNSDDCCFDWNEEKAAELQRTGVSFDRSLTPQSLRTST
RRLSEENKQQSGTMHIDTSPSVVSDIISSRRDRSQDFFGPHSSSPIAPSERQRADQRS
RLESMRLTRRRDKMTKVRGGLEKMEEMIMQGEHLREMQRLKQEAQKNALPSDMAEYME
WQNNEDLEDDELLAFIEKQETYKNELEHFLNNANKNVYENNSYPNSHT"
gene complement(<367838..>372013)
/gene="NUP157"
/locus_tag="YER105C"
/db_xref="GeneID:856842"
mRNA complement(<367838..>372013)
/gene="NUP157"
/locus_tag="YER105C"
/product="Nup157p"
/transcript_id="NM_001178996.1"
/db_xref="GeneID:856842"
CDS complement(367838..372013)
/gene="NUP157"
/locus_tag="YER105C"
/experiment="EXISTENCE:direct assay:GO:0003677 DNA binding
[PMID:24062435]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:24062435]"
/experiment="EXISTENCE:direct assay:GO:0005643 nuclear
pore [PMID:10684247]"
/experiment="EXISTENCE:direct assay:GO:0044611 nuclear
pore inner ring [PMID:18046406]"
/experiment="EXISTENCE:genetic interaction:GO:0017056
structural constituent of nuclear pore [PMID:19414608]"
/experiment="EXISTENCE:genetic interaction:GO:0051292
nuclear pore complex assembly [PMID:19414608]"
/experiment="EXISTENCE:mutant phenotype:GO:0000972
transcription-dependent tethering of RNA polymerase II
gene DNA at nuclear periphery
[PMID:20098417|PMID:20932479]"
/experiment="EXISTENCE:mutant phenotype:GO:0000973
post-transcriptional tethering of RNA polymerase II gene
DNA at nuclear periphery [PMID:20932479]"
/note="Subunit of the inner ring of the nuclear pore
complex (NPC); contributes to NPC assembly and tethering
of DNA to the nuclear periphery; both Nup170p and NUP157p
are similar to human Nup155p; NUP157 has a paralog,
NUP170, that arose from the whole genome duplication"
/codon_start=1
/product="Nup157p"
/protein_id="NP_011031.1"
/db_xref="GeneID:856842"
/db_xref="SGD:S000000907"
/translation="MYSTPLKKRIDYDRETFTASASLGGNRLRNRPRDDQNNGKPNLS
SRSFLSERKTRKDVLNKYGEAGNTIESELRDVTTHVKISGLTSSEPLQLASEFVQDLS
FRDRNTPILDNPDYYSKGLDYNFSDEVGGLGAFTPFQRQQVTNIPDEVLSQVSNTEIK
SDMGIFLELNYCWITSDNKLILWNINNSSEYHCIDEIEHTILKVKLVKPSPNTFVSSV
ENLLIVATLFDIYILTISFNDRTHELNIFNTGLKVNVTGFNVSNIISYERTGQIFFTG
ATDGVNVWELQYNCSENLFNSKSNKICLTKSNLANLLPTKLIPSIPGGKLIQKVLEGD
AGTEEETISQLEVDQSRGVLHTLSTKSIVRSYLITSNGLVGPVLIDAAHIRRGMNALG
VKNSPLLSNRAFKIAKIVSISMCENNDLFLAVITTTGVRLYFKGSISRRSIGSLKLDS
VKFPPTSISSSLEQNKSFIIGHHPLNTHDTGPLSTQKASSTYINTTCASTIISPGIYF
TCVRKRANSGELSKGITNKALLENKEEHKLYVSAPDYGILKNYGKYVENTALLDTTDE
IKEIVPLTRSFNYTSTPQGYANVFASQYSAEPLKVAVLTSNALEIYCYRTPDEVFESL
IENPLPFIHSYGLSEACSTALYLACKFNKSEHIKSSALAFFSAGIPGVVEIKPKSSRE
SGSVPPISQNLFDKSGECDGIVLSPRFYGSALLITRLFSQIWEERVFVFKRASKTEKM
DAFGISITRPQVEYYLSSISVLADFFNIHRPSFVSFVPPKGSNAITASDAESIAMNAL
ILLINSIKDALSLINVFYEDIDAFKSLLNTLMGAGGVYDSKTREYFFDLKFHDLFTPN
AKTKQLIKEILIEVVNANIASGTSADYIVNVLKERFGSFCHSADILCYRAGEHLEAAQ
KFEMIDSKISRNHLDTAIDLYERCAENIELCELRRVVDIMVKLNYQPKTVGFLLRFAD
KIDKGNQAQEYVSRGCNTADPRKVFYDKRINVYTLIFEIVKSVDDYTSIEQSPSIANI
SIFSPASSLKKRVYSVIMNSNNRFFHYCFYDWLVANKRQDYLLRLDSQFVLPYLKERA
EKSLEISNLLWFYLFKEEHFLEAADVLYALASSDFDLKLSERIECLARANGLCDSSTS
FDQKPALVQLSENIHELFDIASIQDDLLNLVRNETRIDEDYRKQLTLKLNGRVLPLSD
LFNDCADPLDYYEIKLRIFKVSQFKDEKVIQGEWNRLLDSMKNAPSPDVGSVGQESFL
SSISNTLIRIGKTTRDTDVVFPVHFLMNKILESFIDKSSAADGSVCSMFLLAGVSHLK
LYYILSRIIENSEGNVELAKKEMVWLIKDWYQSDSDLRGSIAPEQIKKLEKYDPNTDP
VQDYVKDRHHGLK"
gene <372326..>373234
/gene="MAM1"
/locus_tag="YER106W"
/db_xref="GeneID:856843"
mRNA <372326..>373234
/gene="MAM1"
/locus_tag="YER106W"
/product="Mam1p"
/transcript_id="NM_001178997.1"
/db_xref="GeneID:856843"
CDS 372326..373234
/gene="MAM1"
/locus_tag="YER106W"
/experiment="EXISTENCE:direct assay:GO:0000776 kinetochore
[PMID:23861669|PMID:12663816|PMID:11163190]"
/experiment="EXISTENCE:direct assay:GO:0033551 monopolin
complex [PMID:12689592]"
/experiment="EXISTENCE:genetic interaction:GO:0010789
meiotic sister chromatid cohesion involved in meiosis I
[PMID:15062096]"
/experiment="EXISTENCE:genetic interaction:GO:0051455
spindle attachment to meiosis I kinetochore
[PMID:23861669]"
/experiment="EXISTENCE:mutant phenotype:GO:0010789 meiotic
sister chromatid cohesion involved in meiosis I
[PMID:15062096]"
/experiment="EXISTENCE:mutant phenotype:GO:0045132 meiotic
chromosome segregation [PMID:11470404]"
/experiment="EXISTENCE:mutant phenotype:GO:0045143
homologous chromosome segregation [PMID:11163190]"
/experiment="EXISTENCE:mutant phenotype:GO:0051455 spindle
attachment to meiosis I kinetochore [PMID:11163190]"
/note="Monopolin; meiosis-specific kinetochore-associated
protein involved in monopolar attachment of sister
kinetochores to the meiotic spindle; subunit of monopolin,
a complex that prevents biorientation of sister
kinetochores to ensure homolog biorientation during
meiosis I; regulates the conformation, enzyme kinetics and
substrate specificity of the Dsn1p kinase, Hrr1p;
expressed only during the first meiotic division"
/codon_start=1
/product="Mam1p"
/protein_id="NP_011032.1"
/db_xref="GeneID:856843"
/db_xref="SGD:S000000908"
/translation="MREKRTISNKDTNYLKFPNKLQRYSRFLSRKISNTSPEKQPKKN
IKEHCLSSYHKEHSVKPKQNSGNVAAKEDKDTQHLQNNVANEEATECLTRSNLKKLQE
KIFDRELNDIACDHCLCSTENRRDIKYSRLWFLFELEMSENWNENLRLSCYNKYVYSA
IDESWKMENILLKEQEKHYEYFPIGQLLIPNNIDYTNKQKRKENIEDLTIEIDSIIET
NHQKKRFLPQSVLIKREDEIAFDDFHLDARKVLNDLSATSENPFSSSPNTKKIKSKGK
TLEVVPKKKNKKIIGALERKLHIDEN"
gene complement(<373448..>374545)
/gene="GLE2"
/locus_tag="YER107C"
/gene_synonym="RAE1"
/db_xref="GeneID:856844"
mRNA complement(<373448..>374545)
/gene="GLE2"
/locus_tag="YER107C"
/gene_synonym="RAE1"
/product="RNA export factor GLE2"
/transcript_id="NM_001178998.3"
/db_xref="GeneID:856844"
CDS complement(373448..374545)
/gene="GLE2"
/locus_tag="YER107C"
/gene_synonym="RAE1"
/experiment="EXISTENCE:direct assay:GO:0005643 nuclear
pore [PMID:10684247|PMID:8970155]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:15210706]"
/experiment="EXISTENCE:mutant phenotype:GO:0000972
transcription-dependent tethering of RNA polymerase II
gene DNA at nuclear periphery
[PMID:20098417|PMID:20932479]"
/experiment="EXISTENCE:mutant phenotype:GO:0000973
post-transcriptional tethering of RNA polymerase II gene
DNA at nuclear periphery [PMID:20932479]"
/experiment="EXISTENCE:mutant phenotype:GO:0016973
poly(A)+ mRNA export from nucleus [PMID:8970155]"
/experiment="EXISTENCE:mutant phenotype:GO:0032185 septin
cytoskeleton organization [PMID:28776765]"
/experiment="EXISTENCE:mutant phenotype:GO:0051664 nuclear
pore localization [PMID:9725905]"
/experiment="EXISTENCE:mutant phenotype:GO:2000728
regulation of mRNA export from nucleus in response to heat
stress [PMID:15210706]"
/note="RNA export factor associated with the nuclear pore
complex (NPC); associates with NUP116p; required for
polyadenylated RNA export but not for protein import;
homologous to S. pombe Rae1p and human RAE1"
/codon_start=1
/product="RNA export factor GLE2"
/protein_id="NP_011033.3"
/db_xref="GeneID:856844"
/db_xref="SGD:S000000909"
/translation="MSFFNRSNTTSALGTSTAMANEKDLANDIVINSPAEDSISDIAF
SPQQDFMFSASSWDGKVRIWDVQNGVPQGRAQHESSSPVLCTRWSNDGTKVASGGCDN
ALKLYDIASGQTQQIGMHSAPIKVLRFVQCGPSNTECIVTGSWDKTIKYWDMRQPQPV
STVMMPERVYSMDNKQSLLVVATAERHIAIINLANPTTIFKATTSPLKWQTRCVACYN
EADGYAIGSVEGRCSIRYIDDGMQKKSGFSFKCHRQTNPNRAPGSNGQSLVYPVNSIA
FHPLYGTFVTAGGDGTFNFWDKNQRHRLKGYPTLQASIPVCSFNRNGSVFAYALSYDW
HQGHMGNRPDYPNVIRLHATTDEEVKEKKKR"
gene complement(375215..377614)
/gene="FLO8"
/locus_tag="YER109C"
/gene_synonym="PHD5; STA10; YER108C"
/pseudo
/db_xref="GeneID:856845"
CDS complement(375215..377614)
/gene="FLO8"
/locus_tag="YER109C"
/gene_synonym="PHD5; STA10; YER108C"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:12024012]"
/experiment="EXISTENCE:direct assay:GO:0001228 DNA-binding
transcription activator activity, RNA polymerase
II-specific [PMID:10515942]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12024012|PMID:8913742|PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0045944 positive
regulation of transcription by RNA polymerase II
[PMID:12024012]"
/experiment="EXISTENCE:direct assay:GO:2000222 positive
regulation of pseudohyphal growth [PMID:12024012]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:16568252|PMID:10064592|PMID:10591965|PMID:10373537|P
MID:12024012]"
/experiment="EXISTENCE:mutant phenotype:GO:1900189
positive regulation of cell adhesion involved in
single-species biofilm formation [PMID:18202364]"
/experiment="EXISTENCE:mutant phenotype:GO:1900735
positive regulation of flocculation
[PMID:16568252|PMID:10591965]"
/experiment="EXISTENCE:mutant phenotype:GO:2000219
positive regulation of invasive growth in response to
glucose limitation
[PMID:10591965|PMID:10373537|PMID:16568252]"
/experiment="EXISTENCE:mutant phenotype:GO:2000222
positive regulation of pseudohyphal growth
[PMID:10373537|PMID:10591965|PMID:12024012|PMID:10064592]"
/experiment="EXISTENCE:mutant phenotype:GO:2000883
positive regulation of starch catabolic process
[PMID:10591965]"
/note="Transcription factor; required for flocculation,
diploid filamentous growth, and haploid invasive growth;
forms a heterodimer with Mss11p that interacts with the
Swi/Snf complex during transcriptional activation of FLO1,
FLO11, and STA1; S. cerevisiae genome reference strain
S288C contains an internal in-frame stop at codon 142,
which in other strains encodes tryptophan"
/pseudo
/codon_start=1
/db_xref="GeneID:856845"
/db_xref="SGD:S000000911"
gene complement(<378762..>382103)
/gene="KAP123"
/locus_tag="YER110C"
/gene_synonym="YRB4"
/db_xref="GeneID:856846"
mRNA complement(<378762..>382103)
/gene="KAP123"
/locus_tag="YER110C"
/gene_synonym="YRB4"
/product="karyopherin KAP123"
/transcript_id="NM_001179000.1"
/db_xref="GeneID:856846"
CDS complement(378762..382103)
/gene="KAP123"
/locus_tag="YER110C"
/gene_synonym="YRB4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10684247|PMID:9238021]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:10684247|PMID:9238021]"
/experiment="EXISTENCE:direct assay:GO:0006606 protein
import into nucleus [PMID:9321403]"
/experiment="EXISTENCE:direct assay:GO:0008139 nuclear
localization sequence binding [PMID:11694505]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405]"
/experiment="EXISTENCE:direct assay:GO:0061608 nuclear
import signal receptor activity [PMID:9321403]"
/experiment="EXISTENCE:mutant phenotype:GO:0006606 protein
import into nucleus [PMID:11352936]"
/experiment="EXISTENCE:mutant phenotype:GO:0006607
NLS-bearing protein import into nucleus [PMID:11694505]"
/experiment="EXISTENCE:mutant phenotype:GO:0061608 nuclear
import signal receptor activity [PMID:9321403]"
/experiment="EXISTENCE:mutant phenotype:GO:2000220
regulation of pseudohyphal growth [PMID:22043304]"
/experiment="EXISTENCE:physical interaction:GO:0061608
nuclear import signal receptor activity [PMID:9182759]"
/note="Karyopherin beta; mediates nuclear import of
ribosomal proteins prior to assembly into ribosomes and
import of histones H3 and H4; localizes to the nuclear
pore, nucleus, and cytoplasm; exhibits genetic
interactions with RAI1"
/codon_start=1
/product="karyopherin KAP123"
/protein_id="NP_011035.1"
/db_xref="GeneID:856846"
/db_xref="SGD:S000000912"
/translation="MDQQFLSQLEQTLHAITSGVGLKEATKTLQTQFYTQPTTLPALI
HILQNGSDDSLKQLAGVEARKLVSKHWNAIDESTRASIKTSLLQTAFSEPKENVRHSN
ARVIASIGTEELDGNKWPDLVPNLIQTASGEDVQTRQTAIFILFSLLEDFTSSLSGHI
DDFLALFSQTINDPSSLEIRSLSAQALNHVSALIEEQETINPVQAQKFAASIPSVVNV
LDAVIKADDTMNAKLIFNCLNDFLLLDSQLTGNFIVDLIKLSLQIAVNSEIDEDVRVF
ALQFIISSLSYRKSKVSQSKLGPEITVAALKVACEEIDVDDELNNEDETGENEENTPS
SSAIRLLAFASSELPPSQVASVIVEHIPAMLQSANVFERRAILLAISVAVTGSPDYIL
SQFDKIIPATINGLKDTEPIVKLAALKCIHQLTTDLQDEVAKFHEEYLPLIIDIIDSA
KNIVIYNYATVALDGLLEFIAYDAIAKYLDPLMNKLFYMLESNESSKLRCAVVSAIGS
AAFAAGSAFIPYFKTSVHYLEKFIQNCSQIEGMSEDDIELRANTFENISTMARAVRSD
AFAEFAEPLVNSAYEAIKTDSARLRESGYAFIANLAKVYGENFAPFLKTILPEIFKTL
ELDEYQFNFDGDAEDLAAFADSANEEELQNKFTVNTGISYEKEVASAALSELALGTKE
HFLPYVEQSLKVLNEQVDESYGLRETALNTIWNVVKSVLLASKVEPESYPKGIPASSY
VNADVLAVIQAARETSMGNLSDEFETSMVITVMEDFANMIKQFGAIIIMDNGDSSMLE
ALCMQVLSVLKGTHTCQTIDIEEDVPRDEELDASETEATLQDVALEVLVSLSQALAGD
FAKVFDNFRPVVFGLFQSKSKNKRSSAVGAASELALGMKEQNPFVHEMLEALVIRLTS
DKSLEVRGNAAYGVGLLCEYASMDISAVYEPVLKALYELLSAADQKALAAEDDEATRE
IIDRAYANASGCVARMALKNSALVPLEQTVPALLAHLPLNTGFEEYNPIFELIMKLYQ
ENSPVITNETPRIIEIFSAVFTKENDRIKLEKESTLGREENMERLKQFQTEEMKHKVI
ELLKYLNTTYNGIVAQNPVLAAVIA"
gene complement(<382595..>385876)
/gene="SWI4"
/locus_tag="YER111C"
/gene_synonym="ART1"
/db_xref="GeneID:856847"
mRNA complement(<382595..>385876)
/gene="SWI4"
/locus_tag="YER111C"
/gene_synonym="ART1"
/product="SBF complex DNA-binding subunit SWI4"
/transcript_id="NM_001179001.1"
/db_xref="GeneID:856847"
CDS complement(382595..385876)
/gene="SWI4"
/locus_tag="YER111C"
/gene_synonym="ART1"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:12464632]"
/experiment="EXISTENCE:direct assay:GO:0033309 SBF
transcription complex
[PMID:2689885|PMID:8423776|PMID:1832338]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:19111667]"
/experiment="EXISTENCE:mutant phenotype:GO:0000082 G1/S
transition of mitotic cell cycle
[PMID:2649246|PMID:3542227]"
/experiment="EXISTENCE:mutant phenotype:GO:0001228
DNA-binding transcription activator activity, RNA
polymerase II-specific [PMID:8423776|PMID:2649246]"
/experiment="EXISTENCE:mutant phenotype:GO:0033309 SBF
transcription complex [PMID:8423776]"
/experiment="EXISTENCE:mutant phenotype:GO:0034605
cellular response to heat [PMID:20641022]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:2649246|PMID:3542227|PMID:20641022]"
/experiment="EXISTENCE:physical interaction:GO:0033309 SBF
transcription complex [PMID:1465410]"
/note="DNA binding component of the SBF complex
(Swi4p-Swi6p); a transcriptional activator that in concert
with MBF (Mbp1-Swi6p) regulates late G1-specific
transcription of targets including cyclins and genes
required for DNA synthesis and repair; Slt2p-independent
regulator of cold growth; acetylation at two sites, K1016
and K1066, regulates interaction with Swi6p"
/codon_start=1
/product="SBF complex DNA-binding subunit SWI4"
/protein_id="NP_011036.1"
/db_xref="GeneID:856847"
/db_xref="SGD:S000000913"
/translation="MPFDVLISNQKDNTNHQNITPISKSVLLAPHSNHPVIEIATYSE
TDVYECYIRGFETKIVMRRTKDDWINITQVFKIAQFSKTKRTKILEKESNDMQHEKVQ
GGYGRFQGTWIPLDSAKFLVNKYEIIDPVVNSILTFQFDPNNPPPKRSKNSILRKTSP
GTKITSPSSYNKTPRKKNSSSSTSATTTAANKKGKKNASINQPNPSPLQNLVFQTPQQ
FQVNSSMNIMNNNDNHTTMNFNNDTRHNLINNISNNSNQSTIIQQQKSIHENSFNNNY
SATQKPLQFFPIPTNLQNKNVALNNPNNNDSNSYSHNIDNVINSSNNNNNGNNNNLII
VPDGPMQSQQQQQHHHEYLTNNFNHSMMDSITNGNSKKRRKKLNQSNEQQFYNQQEKI
QRHFKLMKQPLLWQSFQNPNDHHNEYCDSNGSNNNNNTVASNGSSIEVFSSNENDNSM
NMSSRSMTPFSAGNTSSQNKLENKMTDQEYKQTILTILSSERSSDVDQALLATLYPAP
KNFNINFEIDDQGHTPLHWATAMANIPLIKMLITLNANALQCNKLGFNCITKSIFYNN
CYKENAFDEIISILKICLITPDVNGRLPFHYLIELSVNKSKNPMIIKSYMDSIILSLG
QQDYNLLKICLNYQDNIGNTPLHLSALNLNFEVYNRLVYLGASTDILNLDNESPASIM
NKFNTPAGGSNSRNNNTKADRKLARNLPQKNYYQQQQQQQQPQNNVKIPKIIKTQHPD
KEDSTADVNIAKTDSEVNESQYLHSNQPNSTNMNTIMEDLSNINSFVTSSVIKDIKST
PSKILENSPILYRRRSQSISDEKEKAKDNENQVEKKKDPLNSVKTAMPSLESPSSLLP
IQMSPLGKYSKPLSQQINKLNTKVSSLQRIMGEEIKNLDNEVVETESSISNNKKRLIT
IAHQIEDAFDSVSNKTPINSISDLQSRIKETSSKLNSEKQNFIQSLEKSQALKLATIV
QDEESKVDMNTNSSSHPEKQEDEEPIPKSTSETSSPKNTKADAKFSNTVQESYDVNET
LRLATELTILQFKRRMTTLKISEAKSKINSSVKLDKYRNLIGITIENIDSKLDDIEKD
LRANA"
gene <387232..>387795
/gene="LSM4"
/locus_tag="YER112W"
/gene_synonym="SDB23; USS1"
/db_xref="GeneID:856848"
mRNA <387232..>387795
/gene="LSM4"
/locus_tag="YER112W"
/gene_synonym="SDB23; USS1"
/product="U6 snRNA complex subunit LSM4"
/transcript_id="NM_001179002.3"
/db_xref="GeneID:856848"
CDS 387232..387795
/gene="LSM4"
/locus_tag="YER112W"
/gene_synonym="SDB23; USS1"
/experiment="EXISTENCE:direct assay:GO:0000932 P-body
[PMID:18611963]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005688 U6 snRNP
[PMID:10369684|PMID:11720284]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:15075370]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0017070 U6 snRNA
binding [PMID:10369684]"
/experiment="EXISTENCE:direct assay:GO:0046540 U4/U6 x U5
tri-snRNP complex [PMID:10449419|PMID:10377396]"
/experiment="EXISTENCE:direct assay:GO:1990726 Lsm1-7-Pat1
complex [PMID:24139796]"
/experiment="EXISTENCE:genetic interaction:GO:0033962
P-body assembly [PMID:17984320|PMID:18981231]"
/experiment="EXISTENCE:genetic interaction:GO:0042149
cellular response to glucose starvation [PMID:18981231]"
/experiment="EXISTENCE:mutant phenotype:GO:0000398 mRNA
splicing, via spliceosome [PMID:10428970]"
/experiment="EXISTENCE:mutant phenotype:GO:0017070 U6
snRNA binding [PMID:10369684]"
/experiment="EXISTENCE:mutant phenotype:GO:0033962 P-body
assembly [PMID:17984320|PMID:18981231]"
/experiment="EXISTENCE:mutant phenotype:GO:0042149
cellular response to glucose starvation [PMID:18981231]"
/experiment="EXISTENCE:physical interaction:GO:0005732
sno(s)RNA-containing ribonucleoprotein complex
[PMID:15075370]"
/note="Lsm (Like Sm) protein; part of heteroheptameric
complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic
Lsm1p complex involved in mRNA decay; nuclear Lsm8p
complex part of U6 snRNP and possibly involved in
processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci
upon DNA replication stress"
/codon_start=1
/product="U6 snRNA complex subunit LSM4"
/protein_id="NP_011037.3"
/db_xref="GeneID:856848"
/db_xref="SGD:S000000914"
/translation="MLPLYLLTNAKGQQMQIELKNGEIIQGILTNVDNWMNLTLSNVT
EYSEESAINSEDNAESSKAVKLNEIYIRGTFIKFIKLQDNIIDKVKQQINSNNNSNSN
GPGHKRYYNNRDSNNNRGNYNRRNNNNGNSNRRPYSQNRQYNNSNSSNINNSINSINS
NNQNMNNGLGGSVQHHFNSSSPQKVEF"
gene complement(<387932..>390052)
/gene="TMN3"
/locus_tag="YER113C"
/db_xref="GeneID:856849"
mRNA complement(<387932..>390052)
/gene="TMN3"
/locus_tag="YER113C"
/product="Tmn3p"
/transcript_id="NM_001179003.3"
/db_xref="GeneID:856849"
CDS complement(387932..390052)
/gene="TMN3"
/locus_tag="YER113C"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0006878
intracellular copper ion homeostasis [PMID:20681974]"
/experiment="EXISTENCE:genetic interaction:GO:0007034
vacuolar transport [PMID:20526336]"
/experiment="EXISTENCE:genetic interaction:GO:0007124
pseudohyphal growth [PMID:18178563]"
/experiment="EXISTENCE:mutant phenotype:GO:0001403
invasive growth in response to glucose limitation
[PMID:18178563]"
/note="Protein with a role in cellular adhesion and
filamentous growth; similar to Emp70p and Tmn2p; member of
Transmembrane Nine family with 9 transmembrane segments;
localizes to Golgi; induced by 8-methoxypsoralen plus UVA
irradiation"
/codon_start=1
/product="Tmn3p"
/protein_id="NP_011038.3"
/db_xref="GeneID:856849"
/db_xref="SGD:S000000915"
/translation="MRVRPKRSVITLMAIVVVMLILRNQFYSSRTRGHGQEPVISSSQ
KNLYDGWITPNFYRKGDPLELIVNKVESDLTQLPYAYYDLPFTCPPTMHKKPLHLSLN
EIIRGDRKWESDYKLKFGEDNPCETLCARKTTKEGMQTLDKLVREGYVVQWLIDDELP
AATTFISTTDHKKYYASGFPLGFIDPDTDKTYLHNHVMLVIRFHASDNDKNTIVGFEV
YPRSVSDYHCPGASKNYEQYEIVIPEDENELTYLPFTYSVYWREEFEVDWNHRWDYFL
NAGELSDEQSIQFHWMSLANSVGIVLSISFITLIIYVRVMYTDKSNSKSPKYMINIEG
IETEDDLDDDKYGKYSVYTVAKDWIQNGRPNLFGLKVLILLVSFGVQFLFTIIGSLTI
SCSMNKLHNVRNSVLTMAILFFVLGAFMASFVGTRLSMVTKTKRTKANYLDDNRYLKD
YKKFSPIFTILCGSSLPGIVMVSTFLLNSIVWAHDSTSALPFKTIVFFMSIYFIVCIP
LSLFGGIVANNIPLPQYWLSGITKDESNSDGNGLFVPKSRAKFNPLVYCGIYLCGIFP
LLVIYVEMQYVYKSLWLEKTTFYYFYGFLFLSIILLCVLTMEISIIGSYLLMRFCFED
KVVRNNWRWKCFEMGFSGGVYMELYSLYYIFAVLNIHGFSSILISICYSLIFNVMCSL
GLGALSYLTASWFINKIYHQKVNL"
gene complement(<390590..>393712)
/gene="BOI2"
/locus_tag="YER114C"
/gene_synonym="BEB1"
/db_xref="GeneID:856850"
mRNA complement(<390590..>393712)
/gene="BOI2"
/locus_tag="YER114C"
/gene_synonym="BEB1"
/product="Boi2p"
/transcript_id="NM_001179004.1"
/db_xref="GeneID:856850"
CDS complement(390590..393712)
/gene="BOI2"
/locus_tag="YER114C"
/gene_synonym="BEB1"
/experiment="EXISTENCE:direct assay:GO:0005543
phospholipid binding [PMID:28751498]"
/experiment="EXISTENCE:direct assay:GO:0005933 cellular
bud [PMID:12097146]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:12097146]"
/experiment="EXISTENCE:direct assay:GO:0030427 site of
polarized growth [PMID:12097146]"
/experiment="EXISTENCE:genetic interaction:GO:0000920
septum digestion after cytokinesis [PMID:16615892]"
/experiment="EXISTENCE:genetic interaction:GO:0007015
actin filament organization [PMID:31964708]"
/experiment="EXISTENCE:genetic interaction:GO:0007118
budding cell apical bud growth [PMID:17417630]"
/experiment="EXISTENCE:mutant phenotype:GO:0099500 vesicle
fusion to plasma membrane [PMID:28751498]"
/note="Protein involved in polar growth; functionally
redundant with Boi1p; required for fusion of secretory
vesicles with plasma membrane at sites of polarized growth
and for inhibition of membrane abscission until the end of
cytokinesis; interacts with bud-emergence protein Bem1p;
contains an SH3 (src homology 3) domain and a PH
(pleckstrin homology) domain; relocalizes from bud neck to
cytoplasm upon DNA replication stress; BOI2 has a paralog,
BOI1, that arose from the whole genome duplication"
/codon_start=1
/product="Boi2p"
/protein_id="NP_011039.1"
/db_xref="GeneID:856850"
/db_xref="SGD:S000000916"
/translation="MSNDREVPTLSQLNTTVSRDKDVSDTLSPDFDSKGSATGRDGGN
FPMYIAINEYFKRMEDELDMKPGDKIKVITDDEEYKDGWYFGRNLRTNEEGLYPVVFT
QKITVEKAPTLMRAKSTKRIYSPLTNEDPLLSSTFISENDSNSELPTPQPIETAASIS
RTANGKIERNLSLKNTMSDIDNALLEFKDDSIGPPDRFINSGRDEEHSITHETILSAT
DGLDVVESNSKPTTSSSTGFLNGDLENQATLINGIDTTKLNPVEAEFWSPEEITAYFI
MEGYDVQSASRFQKHKISGKILLELELVHLKELDINSFGTRFEIFKEIEKIKEAIRTN
GRSLNRASKTNNANIYNQLMPPANVDQRASYRGHVRKTSQSLEDLPSQQNFIPTPRNT
RNSSASKHRPKSLVFDSQEANANIAPDVQIPQVVEEMAGNENLFVSPRRAPKPPSYPS
PAQPPKSPLLNNTRTSPSPAQLYSWQSPTLSFSGPKRTSYIDQYSSSDSNFNSRSALP
KNNQGGGKALSPIPSPTRNSVRNEDSEGKLTSSSKRNSVPYYGYAPESSSDRKSSCSS
HEEEQFQETMNTFERPTSSIYADGSTIASISNDKLAHEKEGKKKPTRHSSSLSSKSKS
DSRRNSSLKRSSSASRTSSFKKSSFMLSPFRQQFTDNAARSSSPEENPITSMPSEKNS
SPIVDKKSSKKSRSKRRSVSAKEAEIFTETVKDDKNKRSASEAIKGETLKGKSLRQMT
ARPVAKKKQTSAFIEGLRSISVKEAMKDADFSGWMSKKGSGAMSTWKTRFFTLHGTRL
SYFSSTTDTRERGLIDITAHRVVPAKEDDKLVSLYAASTGKGRYCFKLLPPQPGSKKG
LTFTQPRTHYFAVDNKEEMRGWMAALIKTTIDIDTSVPIISSYTTPTVSLSKAQEMLA
EAREETKLREQQMLENEEDEDQFLWDQQQLQQQQHDNNQGQADRTISASTQRTSDEDN
TISTPNLSSANNTTIGSNGFSSPFLLASGLLSPGVARNSSMRGTEKKGKFSTEEDYFG
DNSKHKTDKI"
gene complement(<394292..>394867)
/gene="SPR6"
/locus_tag="YER115C"
/db_xref="GeneID:856851"
mRNA complement(<394292..>394867)
/gene="SPR6"
/locus_tag="YER115C"
/product="Spr6p"
/transcript_id="NM_001179005.1"
/db_xref="GeneID:856851"
CDS complement(394292..394867)
/gene="SPR6"
/locus_tag="YER115C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24390141]"
/experiment="EXISTENCE:expression pattern:GO:0030437
ascospore formation [PMID:2253272]"
/note="hypothetical protein; expressed during sporulation;
not required for sporulation, but gene exhibits genetic
interactions with other genes required for sporulation"
/codon_start=1
/product="Spr6p"
/protein_id="NP_011040.1"
/db_xref="GeneID:856851"
/db_xref="SGD:S000000917"
/translation="MAVSNIWQSYSSSNLHWIYPLYTNNCSQNVKSSFTAEILLKRRC
NDIQDILNDRMIELLLQGACDPNKQQNYLQGMSPSRKKKTHVKKFLKKQKKSRKPITL
EHGCLSGPVTLRFGNFAGIRDLRGTRCPLHGIKHGVHPKPGERCACQQATLFPSPLAR
FSCDQSAVLGCAASSTRVYDSIADEFSSLYF"
gene complement(<395348..>396172)
/gene="SLX8"
/locus_tag="YER116C"
/db_xref="GeneID:856852"
mRNA complement(<395348..>396172)
/gene="SLX8"
/locus_tag="YER116C"
/product="SUMO-targeted ubiquitin ligase complex subunit
SLX8"
/transcript_id="NM_001179006.3"
/db_xref="GeneID:856852"
CDS complement(395348..396172)
/gene="SLX8"
/locus_tag="YER116C"
/EC_number="2.3.2.27"
/experiment="EXISTENCE:direct assay:GO:0000776 kinetochore
[PMID:26906737]"
/experiment="EXISTENCE:direct assay:GO:0004842
ubiquitin-protein transferase activity
[PMID:18032921|PMID:17728242|PMID:17848550|PMID:26906737]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922|PMID:26906737|PMID:19270524]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0016567 protein
ubiquitination
[PMID:17728242|PMID:17848550|PMID:26906737|PMID:18032921]"
/experiment="EXISTENCE:direct assay:GO:0033768
SUMO-targeted ubiquitin ligase complex [PMID:17848550]"
/experiment="EXISTENCE:genetic interaction:GO:0006974 DNA
damage response [PMID:11139495]"
/experiment="EXISTENCE:genetic interaction:GO:0016567
protein ubiquitination [PMID:26906737]"
/experiment="EXISTENCE:genetic interaction:GO:0016925
protein sumoylation [PMID:16387868]"
/experiment="EXISTENCE:mutant phenotype:GO:0000723
telomere maintenance [PMID:16428246]"
/experiment="EXISTENCE:mutant phenotype:GO:0006974 DNA
damage response [PMID:16325482|PMID:11139495]"
/experiment="EXISTENCE:mutant phenotype:GO:0016567 protein
ubiquitination [PMID:26906737]"
/experiment="EXISTENCE:mutant phenotype:GO:0016925 protein
sumoylation [PMID:16387868]"
/note="Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase
(STUbL) complex; role in proteolysis of spindle
positioning protein Kar9, DNA repair proteins Rad52p and
Rad57p; stimulated by SUMO-modified substrates; contains a
C-terminal RING domain; forms nuclear foci upon DNA
replication stress; required for maintenance of genome
integrity like human ortholog RNF4"
/codon_start=1
/product="SUMO-targeted ubiquitin ligase complex subunit
SLX8"
/protein_id="NP_011041.3"
/db_xref="GeneID:856852"
/db_xref="SGD:S000000918"
/translation="MARRPDNQNPEGENLRIKRVRLESVRQNDEEEENEVSRTQNIVT
DNRHDSPEAVVEIIGERALENTSEEDGDDDLSLFRALEEDPGSDHNTSNNDSGNHDRE
TMHTEEPEASSGNNITLTNNVEELHTMDVLSQTANTPSASPMLDAAPPTTKPGTNSKE
QTVDLTADAIDLDAEEQQVLQISDDDFQEETKEAPKEYGAAKDYRCPICFEPPETALM
TLCGHVFCCPCLFQMVNSSRTCRQFGHCALCRSKVYLKDVRLIILRKKQVKKKVKS"
gene <396769..>397653
/gene="RPL23B"
/locus_tag="YER117W"
/db_xref="GeneID:856853"
mRNA join(<396769..396810,397282..>397653)
/gene="RPL23B"
/locus_tag="YER117W"
/product="60S ribosomal protein uL14 RPL23B"
/transcript_id="NM_001179007.3"
/db_xref="GeneID:856853"
CDS join(396769..396810,397282..397653)
/gene="RPL23B"
/locus_tag="YER117W"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:11983894]"
/experiment="EXISTENCE:curator inference:GO:0003735
structural constituent of ribosome [PMID:11983894]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:11983894]"
/note="Ribosomal 60S subunit protein L23B; homologous to
mammalian ribosomal protein L23 and bacterial L14; RPL23B
has a paralog, RPL23A, that arose from the whole genome
duplication"
/codon_start=1
/product="60S ribosomal protein uL14 RPL23B"
/protein_id="NP_011042.3"
/db_xref="GeneID:856853"
/db_xref="SGD:S000000919"
/translation="MSGNGAQGTKFRISLGLPVGAIMNCADNSGARNLYIIAVKGSGS
RLNRLPAASLGDMVMATVKKGKPELRKKVMPAIVVRQAKSWRRRDGVFLYFEDNAGVI
ANPKGEMKGSAITGPVGKECADLWPRVASNSGVVV"
gene complement(<397952..>399055)
/gene="SHO1"
/locus_tag="YER118C"
/gene_synonym="SSU81"
/db_xref="GeneID:856854"
mRNA complement(<397952..>399055)
/gene="SHO1"
/locus_tag="YER118C"
/gene_synonym="SSU81"
/product="osmosensor SHO1"
/transcript_id="NM_001179008.1"
/db_xref="GeneID:856854"
CDS complement(397952..399055)
/gene="SHO1"
/locus_tag="YER118C"
/gene_synonym="SSU81"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:10980703]"
/experiment="EXISTENCE:direct assay:GO:0005933 cellular
bud [PMID:10980703]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:10980703|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:12374868]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0007231
osmosensory signaling pathway [PMID:7624781]"
/experiment="EXISTENCE:genetic interaction:GO:0030010
establishment of cell polarity [PMID:19439450]"
/experiment="EXISTENCE:mutant phenotype:GO:0001402 signal
transduction involved in filamentous growth
[PMID:18591427|PMID:9744864]"
/experiment="EXISTENCE:mutant phenotype:GO:0005078
MAP-kinase scaffold activity [PMID:15200958]"
/experiment="EXISTENCE:mutant phenotype:GO:0007232
osmosensory signaling pathway via Sho1 osmosensor
[PMID:15200958]"
/experiment="EXISTENCE:physical interaction:GO:0005034
osmosensor activity [PMID:7624781]"
/experiment="EXISTENCE:physical interaction:GO:0005078
MAP-kinase scaffold activity
[PMID:16778768|PMID:15200958]"
/experiment="EXISTENCE:physical interaction:GO:0007231
osmosensory signaling pathway [PMID:7624781]"
/experiment="EXISTENCE:physical interaction:GO:0007232
osmosensory signaling pathway via Sho1 osmosensor
[PMID:15200958|PMID:16778768]"
/experiment="EXISTENCE:physical interaction:GO:0044697
HICS complex [PMID:22623719]"
/note="Transmembrane osmosensor for filamentous growth and
HOG pathways; involved in activation of the Cdc42p- and
MAP kinase-dependent filamentous growth pathway and the
high-osmolarity glycerol (HOG) response pathway;
phosphorylated by Hog1p; interacts with Pbs2p, Msb2p,
Hkr1p, and Ste11p"
/codon_start=1
/product="osmosensor SHO1"
/protein_id="NP_011043.1"
/db_xref="GeneID:856854"
/db_xref="SGD:S000000920"
/translation="MSISSKIRPTPRKPSRMATDHSFKMKKFYADPFAISSISLAIVS
WVIAIGGSISSASTNESFPRFTWWGIVYQFLIICSLMLFYCFDLVDHYRIFITTSIAV
AFVYNTNSATNLVYADGPKKAAASAGVILLSIINLIWILYYGGDNASPTNRWIDSFSI
KGIRPSPLENSLHRARRRGNRNTTPYQNNVYNDAIRDSGYATQFDGYPQQQPSHTNYV
SSTALAGFENTQPNTSEAVNLHLNTLQQRINSASNAKETNDNSNNQTNTNIGNTFDTD
FSNGNTETTMGDTLGLYSDIGDDNFIYKAKALYPYDADDDDAYEISFEQNEILQVSDI
EGRWWKARRANGETGIIPSNYVQLIDGPEEMHR"
gene complement(<399496..>400842)
/gene="AVT6"
/locus_tag="YER119C"
/db_xref="GeneID:856855"
mRNA complement(<399496..>400842)
/gene="AVT6"
/locus_tag="YER119C"
/product="aspartate/glutamate transporter"
/transcript_id="NM_001179009.1"
/db_xref="GeneID:856855"
CDS complement(399496..400842)
/gene="AVT6"
/locus_tag="YER119C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:11274162]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762|PMID:20118605]"
/experiment="EXISTENCE:mutant phenotype:GO:0005313
L-glutamate transmembrane transporter activity
[PMID:11274162]"
/experiment="EXISTENCE:mutant phenotype:GO:0015183
L-aspartate transmembrane transporter activity
[PMID:11274162]"
/experiment="EXISTENCE:mutant phenotype:GO:0032974 amino
acid transmembrane export from vacuole [PMID:11274162]"
/experiment="EXISTENCE:mutant phenotype:GO:0055085
transmembrane transport [PMID:11274162]"
/note="Vacuolar aspartate and glutamate exporter; member
of a family of seven genes (AVT1-7) related to vesicular
GABA-glycine transporters; involved in compartmentalizing
acidic amino acids in response to nitrogen starvation;
AVT6 has a paralog, AVT5, that arose from the whole genome
duplication"
/codon_start=1
/product="aspartate/glutamate transporter"
/protein_id="NP_011044.1"
/db_xref="GeneID:856855"
/db_xref="SGD:S000000921"
/translation="MVASIRSGVLTLLHTACGAGILAMPYAFKPFGLIPGVIMIVLCG
ACAMQSLFIQARVAKYVPQGRASFSALTRLINPNLGIVFDLAIAIKCFGVGVSYMIVV
GDLMPQIMSVWTRNAWLLNRNVQISLIMLFFVAPLSFLKKLNSLRYASMVAISSVAYL
CVLVLLHYVAPSDEILRLKGRISYLLPPQSHDLNVLNTLPIFVFAYTCHHNMFSIINE
QRSSRFEHVMKIPLIAISLALILYIAIGCAGYLTFGDNIIGNIIMLYPQAVSSTIGRI
AIVLLVMLAFPLQCHPARASIHQILQHFAEENVSISATSADEPTVATESSPLIRDSSL
DLNEVIEEESIYQPKETPLRGKSFIVITCSILVASYLVAISVSSLARVLAIVGATGST
SISFILPGLFGYKLIGTEHKTAVPLTTKIFKYTGLLLFIWGLIIMITCLTAALKLN"
gene <401135..>401869
/gene="SCS2"
/locus_tag="YER120W"
/db_xref="GeneID:856856"
mRNA <401135..>401869
/gene="SCS2"
/locus_tag="YER120W"
/product="phosphatidylinositol-binding protein SCS2"
/transcript_id="NM_001179010.3"
/db_xref="GeneID:856856"
CDS 401135..401869
/gene="SCS2"
/locus_tag="YER120W"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:12727870|PMID:25420878]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:12727870|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:9537365]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:25420878]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:17984322]"
/experiment="EXISTENCE:direct assay:GO:0031965 nuclear
membrane [PMID:9537365]"
/experiment="EXISTENCE:direct assay:GO:0035091
phosphatidylinositol binding [PMID:17963691]"
/experiment="EXISTENCE:direct assay:GO:0061163 endoplasmic
reticulum polarization [PMID:25083872]"
/experiment="EXISTENCE:direct assay:GO:0071561
nucleus-vacuole junction [PMID:25420878]"
/experiment="EXISTENCE:genetic interaction:GO:0008654
phospholipid biosynthetic process [PMID:8537323]"
/experiment="EXISTENCE:genetic interaction:GO:0031509
subtelomeric heterochromatin formation
[PMID:11333225|PMID:12399377]"
/experiment="EXISTENCE:genetic interaction:GO:0048309
endoplasmic reticulum inheritance [PMID:17984322]"
/experiment="EXISTENCE:genetic interaction:GO:0060304
regulation of phosphatidylinositol dephosphorylation
[PMID:23237950]"
/experiment="EXISTENCE:genetic interaction:GO:0061709
reticulophagy [PMID:35101986]"
/experiment="EXISTENCE:genetic interaction:GO:0090158
endoplasmic reticulum membrane organization
[PMID:23237950]"
/experiment="EXISTENCE:mutant phenotype:GO:0008654
phospholipid biosynthetic process
[PMID:9537365|PMID:12761300]"
/experiment="EXISTENCE:mutant phenotype:GO:0031509
subtelomeric heterochromatin formation [PMID:11333225]"
/experiment="EXISTENCE:mutant phenotype:GO:0032377
regulation of intracellular lipid transport
[PMID:12727870]"
/experiment="EXISTENCE:mutant phenotype:GO:0033149 FFAT
motif binding [PMID:12727870]"
/experiment="EXISTENCE:mutant phenotype:GO:0042308
negative regulation of protein import into nucleus
[PMID:12727870]"
/experiment="EXISTENCE:mutant phenotype:GO:0048309
endoplasmic reticulum inheritance [PMID:17984322]"
/experiment="EXISTENCE:physical interaction:GO:0005935
cellular bud neck [PMID:25083872]"
/experiment="EXISTENCE:physical interaction:GO:0033149
FFAT motif binding [PMID:12727870]"
/experiment="EXISTENCE:physical interaction:GO:0042308
negative regulation of protein import into nucleus
[PMID:12727870]"
/note="Integral ER membrane protein, regulates
phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p,
Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect the ER to the
plasma membrane (PM); regulates PI4P levels by controlling
access of Sac1p to PM substrate PI4P; interacts with FFAT
motifs in Opi1p, Swh1p, Osh2p and Osh3p; role in NVJ
integrity and micronucleophagy; role in telomeric
silencing; VAMP-associated protein (VAP) homolog; SCS2 has
a paralog, SCS22, that arose from the whole genome
duplication"
/codon_start=1
/product="phosphatidylinositol-binding protein SCS2"
/protein_id="NP_011046.3"
/db_xref="GeneID:856856"
/db_xref="SGD:S000000922"
/translation="MSAVEISPDVLVYKSPLTEQSTEYASISNNSDQTIAFKVKTTAP
KFYCVRPNAAVVAPGETIQVQVIFLGLTEEPAADFKCRDKFLVITLPSPYDLNGKAVA
DVWSDLEAEFKQQAISKKIKVKYLISPDVHPAQNQNIQENKETVEPVVQDSEPKEVPA
VVNEKEVPAEPETQPPVQVKKEEVPPVVQKTVPHENEKQTSNSTPAPQNQIKEAATVP
AENESSSMGIFILVALLILVLGWFYR"
gene <402375..>402719
/locus_tag="YER121W"
/db_xref="GeneID:856858"
mRNA <402375..>402719
/locus_tag="YER121W"
/product="uncharacterized protein"
/transcript_id="NM_001179011.3"
/db_xref="GeneID:856858"
CDS 402375..402719
/locus_tag="YER121W"
/note="hypothetical protein; may be involved in
phosphatase regulation and/or generation of precursor
metabolites and energy"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011047.3"
/db_xref="GeneID:856858"
/db_xref="SGD:S000000923"
/translation="MGLSRWHDKNSRPAEEKSEEMQQDAHYYALAASDSLNASVSNEY
GNQVMNSFWKVGIDSPYVDDEAIRNRDVENNLPSLKQSVYNANEPNATSSAFSTASYA
HETFDFRNLKLR"
gene complement(<402871..>404352)
/gene="GLO3"
/locus_tag="YER122C"
/db_xref="GeneID:856859"
mRNA complement(<402871..>404352)
/gene="GLO3"
/locus_tag="YER122C"
/product="ADP-ribosylation factor GTPase-activating
protein"
/transcript_id="NM_001179012.1"
/db_xref="GeneID:856859"
CDS complement(402871..404352)
/gene="GLO3"
/locus_tag="YER122C"
/experiment="EXISTENCE:direct assay:GO:0005096 GTPase
activator activity [PMID:9927415]"
/experiment="EXISTENCE:direct assay:GO:0006890 retrograde
vesicle-mediated transport, Golgi to endoplasmic reticulum
[PMID:9927415]"
/experiment="EXISTENCE:direct assay:GO:0030137 COPI-coated
vesicle [PMID:15254269]"
/experiment="EXISTENCE:direct assay:GO:0048205 COPI
coating of Golgi vesicle [PMID:15254269]"
/experiment="EXISTENCE:mutant phenotype:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:9927415]"
/experiment="EXISTENCE:mutant phenotype:GO:0006890
retrograde vesicle-mediated transport, Golgi to
endoplasmic reticulum [PMID:9927415]"
/experiment="EXISTENCE:mutant phenotype:GO:0048205 COPI
coating of Golgi vesicle [PMID:15254269]"
/experiment="EXISTENCE:physical interaction:GO:0030126
COPI vesicle coat [PMID:15254269]"
/note="ADP-ribosylation factor GTPase activating protein
(ARF GAP); involved in ER-Golgi transport; shares
functional similarity with Gcs1p"
/codon_start=1
/product="ADP-ribosylation factor GTPase-activating
protein"
/protein_id="NP_011048.1"
/db_xref="GeneID:856859"
/db_xref="SGD:S000000924"
/translation="MSNDEGETFATEQTTQQVFQKLGSNMENRVCFDCGNKNPTWTSV
PFGVMLCIQCSAVHRNMGVHITFVKSSTLDKWTINNLRRFKLGGNHKARDFFLKNNGK
QLLNTANVDAKTKYTSPVAKKYKIHLDKKVQKDMELYPSELVLNGQDSSDSPLDTDSD
ASRSTSKENSVDDFFSNWQKPSSNSSSKLNVNTGSLAPKNNTTGSTPKTTVTKTRSSI
LTASRKKPVLNSQDKKKHSILSSSRKPTRLTAKKVDKSQAEDLFDQFKKEAQQEKEDE
FTNSSSSTKIRQNDYDSQFMNNSKGNNNNSIDDINTQPDEFNDFLNDTSNSFDTTRKE
QQDTLTPKFAKLGFGMTMNDANDLAKQQKESQKIAQGPRYTGRIAERYGTQKAISSDQ
LFGRGSFDEAANREAHDKLKTFDNATSISSSSYFGEDKEVDEFGNPINSSGSGAGNFD
GRNSNNGFIDFNASADDELQMLRDVVEQGAEKLGSYLRDYLRK"
gene <404813..>406387
/gene="YCK3"
/locus_tag="YER123W"
/gene_synonym="CKI3"
/db_xref="GeneID:856860"
mRNA <404813..>406387
/gene="YCK3"
/locus_tag="YER123W"
/gene_synonym="CKI3"
/product="casein kinase YCK3"
/transcript_id="NM_001179013.1"
/db_xref="GeneID:856860"
CDS 404813..406387
/gene="YCK3"
/locus_tag="YER123W"
/gene_synonym="CKI3"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:16301533]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane
[PMID:24390141|PMID:26928762|PMID:14668479]"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:8816449]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:8816449]"
/experiment="EXISTENCE:mutant phenotype:GO:0016192
vesicle-mediated transport [PMID:19116312]"
/note="Palmitoylated vacuolar membrane-localized casein
kinase I isoform; negatively regulates vacuole fusion
during hypertonic stress via phosphorylation of Vps41p;
shares essential functions with Hrr25p; targeted to
vacuole via AP-3 pathway; regulates vesicle fusion in AP-3
pathway"
/codon_start=1
/product="casein kinase YCK3"
/protein_id="NP_011049.2"
/db_xref="GeneID:856860"
/db_xref="SGD:S000000925"
/translation="MSQRSSQHIVGIHYAVGPKIGEGSFGVIFEGENILHSCQAQTGS
KRDSSIIMANEPVAIKFEPRHSDAPQLRDEFRAYRILNGCVGIPHAYYFGQEGMHNIL
IIDLLGPSLEDLFEWCGRKFSVKTTCMVAKQMIDRVRAIHDHDLIYRDIKPDNFLISQ
YQRISPEGKVIKSCASSSNNDPNLIYMVDFGMAKQYRDPRTKQHIPYRERKSLSGTAR
YMSINTHFGREQSRRDDLESLGHVFFYFLRGSLPWQGLKAPNNKLKYEKIGMTKQKLN
PDDLLLNNAIPYQFATYLKYARSLKFDEDPDYDYLISLMDDALRLNDLKDDGHYDWMD
LNGGKGWNIKINRRANLHGYGNPNPRVNGNTARNNVNTNSKTRNTTPVATPKQQAQNS
YNKDNSKSRISSNPQSFTKQQHVLKKIEPNSKYIPETHSNLQRPIKSQSQTYDSISHT
QNSPFVPYSSSKANPKRSNNEHNLPNHYTNLANKNINYQSQRNYEQENDAYSDDENDT
FCSKIYKYCCCCFCCC"
rep_origin 406752..406953
/note="ARS517; Autonomously Replicating Sequence"
/db_xref="SGD:S000077389"
gene complement(<407342..>409063)
/gene="DSE1"
/locus_tag="YER124C"
/db_xref="GeneID:856861"
mRNA complement(<407342..>409063)
/gene="DSE1"
/locus_tag="YER124C"
/product="Dse1p"
/transcript_id="NM_001179014.3"
/db_xref="GeneID:856861"
CDS complement(407342..409063)
/gene="DSE1"
/locus_tag="YER124C"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0031683 G-protein
beta/gamma-subunit complex binding [PMID:19820940]"
/experiment="EXISTENCE:mutant phenotype:GO:0000920 septum
digestion after cytokinesis [PMID:11309124]"
/experiment="EXISTENCE:mutant phenotype:GO:0001403
invasive growth in response to glucose limitation
[PMID:19820940]"
/experiment="EXISTENCE:mutant phenotype:GO:0010969
regulation of pheromone-dependent signal transduction
involved in conjugation with cellular fusion
[PMID:19820940]"
/note="Daughter cell-specific protein; may regulate
cross-talk between the mating and filamentation pathways;
deletion affects cell separation after division and
sensitivity to alpha-factor and drugs affecting the cell
wall; relocalizes from bud neck to cytoplasm upon DNA
replication stress"
/codon_start=1
/product="Dse1p"
/protein_id="NP_011050.3"
/db_xref="GeneID:856861"
/db_xref="SGD:S000000926"
/translation="MQDTKYYEPTNIFRQPAINIKKRSDKKRILQSMMTLSTYKKTWQ
NNTSKMNSPILRKASDNFNDYYTTKKLKSDYWKLYGVDESELSIPSDMSIVDNILLVS
TMNEKDNLKLFEISAEKKLKELQTITVPGKPITCICLLPMVDFPPQIFPSSQINPNHN
QLILTGHQDGIVNLIATSTYKGCAKIIKRFNHNKFLKSTVSTSIPILEITPKTAPILK
VSPWNKTGFVSLLNDSLFIYDLKSNLDCIKTPIFLQSYPGINSFAVNEFHDPFLLALV
GSKFGPNGISLLDLRTNLYIPDILDNSISAGCGKDHLQRKNTSLDCVWISNHHVAQSL
NDKIQIWDIQSCDGKPVCELYAKKGYIESLKFNENTGALYSSDDQGFVICWDLQNLQN
MKYGELVHGFNSISLDSESELLLTKQVFQCGNIIVSGMSDKNICLKSNDTKANGKGCG
FLFLDMANDGSLVTLDNFCELGLHQICQVQFNVDTGKIIDGNGTGKSGISDSSMLSLS
NESDHSMTETSDDMFSNSGNWDCSSANTVSEGRLNDDQEDIVFTKRMYSVNDVHLSGS
TIDTTVV"
gene <410189..>412618
/gene="RSP5"
/locus_tag="YER125W"
/gene_synonym="HPG1; MDP1; MUT2; NPI1; SMM1; UBY1"
/db_xref="GeneID:856862"
mRNA <410189..>412618
/gene="RSP5"
/locus_tag="YER125W"
/gene_synonym="HPG1; MDP1; MUT2; NPI1; SMM1; UBY1"
/product="NEDD4 family E3 ubiquitin-protein ligase"
/transcript_id="NM_001179015.3"
/db_xref="GeneID:856862"
CDS 410189..412618
/gene="RSP5"
/locus_tag="YER125W"
/gene_synonym="HPG1; MDP1; MUT2; NPI1; SMM1; UBY1"
/EC_number="2.3.2.26"
/experiment="EXISTENCE:direct assay:GO:0004842
ubiquitin-protein transferase activity
[PMID:9108033|PMID:21685393|PMID:23443663|PMID:27694912]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14608372]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:15078904|PMID:14657247]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:14657247]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:14608372]"
/experiment="EXISTENCE:direct assay:GO:0006515 protein
quality control for misfolded or incompletely synthesized
proteins [PMID:30361468]"
/experiment="EXISTENCE:direct assay:GO:0010008 endosome
membrane [PMID:14657247]"
/experiment="EXISTENCE:direct assay:GO:0010994 free
ubiquitin chain polymerization [PMID:19153599]"
/experiment="EXISTENCE:direct assay:GO:0016567 protein
ubiquitination [PMID:23443663]"
/experiment="EXISTENCE:direct assay:GO:0022626 cytosolic
ribosome [PMID:30893611]"
/experiment="EXISTENCE:direct assay:GO:0034450
ubiquitin-ubiquitin ligase activity [PMID:19153599]"
/experiment="EXISTENCE:direct assay:GO:0035091
phosphatidylinositol binding [PMID:15078904]"
/experiment="EXISTENCE:direct assay:GO:0043130 ubiquitin
binding [PMID:19252184]"
/experiment="EXISTENCE:direct assay:GO:0061630 ubiquitin
protein ligase activity [PMID:30893611]"
/experiment="EXISTENCE:direct assay:GO:0070534 protein
K63-linked ubiquitination [PMID:30893611]"
/experiment="EXISTENCE:direct assay:GO:0070651
nonfunctional rRNA decay [PMID:30893611]"
/experiment="EXISTENCE:genetic interaction:GO:0006808
regulation of nitrogen utilization [PMID:15247235]"
/experiment="EXISTENCE:genetic interaction:GO:0007005
mitochondrion organization [PMID:10366593]"
/experiment="EXISTENCE:genetic interaction:GO:0010794
regulation of dolichyl monophosphate biosynthetic process
[PMID:18771750]"
/experiment="EXISTENCE:genetic interaction:GO:0010795
regulation of ubiquinone biosynthetic process
[PMID:18771750]"
/experiment="EXISTENCE:genetic interaction:GO:0032443
regulation of ergosterol biosynthetic process
[PMID:18771750]"
/experiment="EXISTENCE:genetic interaction:GO:0032956
regulation of actin cytoskeleton organization
[PMID:15855235]"
/experiment="EXISTENCE:genetic interaction:GO:0034517
ribophagy [PMID:18670191]"
/experiment="EXISTENCE:mutant phenotype:GO:0004842
ubiquitin-protein transferase activity
[PMID:9108033|PMID:27694912|PMID:21685393]"
/experiment="EXISTENCE:mutant phenotype:GO:0005829 cytosol
[PMID:25344756]"
/experiment="EXISTENCE:mutant phenotype:GO:0006325
chromatin organization [PMID:12399376]"
/experiment="EXISTENCE:mutant phenotype:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:9614172|PMID:25344756]"
/experiment="EXISTENCE:mutant phenotype:GO:0007005
mitochondrion organization [PMID:10366593]"
/experiment="EXISTENCE:mutant phenotype:GO:0010793
regulation of mRNA export from nucleus [PMID:14608372]"
/experiment="EXISTENCE:mutant phenotype:GO:0010794
regulation of dolichyl monophosphate biosynthetic process
[PMID:18771750]"
/experiment="EXISTENCE:mutant phenotype:GO:0010795
regulation of ubiquinone biosynthetic process
[PMID:18771750]"
/experiment="EXISTENCE:mutant phenotype:GO:0016567 protein
ubiquitination [PMID:29444958]"
/experiment="EXISTENCE:mutant phenotype:GO:0016973
poly(A)+ mRNA export from nucleus [PMID:27385342]"
/experiment="EXISTENCE:mutant phenotype:GO:0032436
positive regulation of proteasomal ubiquitin-dependent
protein catabolic process [PMID:24962567]"
/experiment="EXISTENCE:mutant phenotype:GO:0032443
regulation of ergosterol biosynthetic process
[PMID:18771750]"
/experiment="EXISTENCE:mutant phenotype:GO:0032511 late
endosome to vacuole transport via multivesicular body
sorting pathway [PMID:17182849]"
/experiment="EXISTENCE:mutant phenotype:GO:0032880
regulation of protein localization [PMID:12867034]"
/experiment="EXISTENCE:mutant phenotype:GO:0033554
cellular response to stress [PMID:31658248]"
/experiment="EXISTENCE:mutant phenotype:GO:0034605
cellular response to heat [PMID:24962567|PMID:25344756]"
/experiment="EXISTENCE:mutant phenotype:GO:0043162
ubiquitin-dependent protein catabolic process via the
multivesicular body sorting pathway [PMID:17344478]"
/experiment="EXISTENCE:mutant phenotype:GO:0043328 protein
transport to vacuole involved in ubiquitin-dependent
protein catabolic process via the multivesicular body
sorting pathway [PMID:29444958]"
/experiment="EXISTENCE:mutant phenotype:GO:0045723
positive regulation of fatty acid biosynthetic process
[PMID:11007476]"
/experiment="EXISTENCE:mutant phenotype:GO:0045807
positive regulation of endocytosis [PMID:12654912]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:11007476]"
/experiment="EXISTENCE:mutant phenotype:GO:0048260
positive regulation of receptor-mediated endocytosis
[PMID:11179425]"
/experiment="EXISTENCE:mutant phenotype:GO:0070086
ubiquitin-dependent endocytosis
[PMID:17344478|PMID:31658248|PMID:32800549]"
/experiment="EXISTENCE:mutant phenotype:GO:0071230
cellular response to amino acid stimulus [PMID:31658248]"
/experiment="EXISTENCE:mutant phenotype:GO:0072671
mitochondria-associated ubiquitin-dependent protein
catabolic process [PMID:21070972]"
/experiment="EXISTENCE:mutant phenotype:GO:1903577
cellular response to L-arginine [PMID:32800549]"
/experiment="EXISTENCE:mutant phenotype:GO:2000203
regulation of ribosomal large subunit export from nucleus
[PMID:14608372]"
/experiment="EXISTENCE:mutant phenotype:GO:2000232
regulation of rRNA processing [PMID:14608372]"
/experiment="EXISTENCE:mutant phenotype:GO:2000235
regulation of tRNA processing [PMID:14608372]"
/experiment="EXISTENCE:mutant phenotype:GO:2000238
regulation of tRNA export from nucleus [PMID:14608372]"
/experiment="EXISTENCE:physical interaction:GO:0000151
ubiquitin ligase complex [PMID:9931424]"
/experiment="EXISTENCE:physical interaction:GO:0010793
regulation of mRNA export from nucleus [PMID:15713680]"
/experiment="EXISTENCE:physical interaction:GO:0032511
late endosome to vacuole transport via multivesicular body
sorting pathway [PMID:17182849]"
/experiment="EXISTENCE:physical interaction:GO:0032880
regulation of protein localization [PMID:12867034]"
/experiment="EXISTENCE:physical interaction:GO:0043161
proteasome-mediated ubiquitin-dependent protein catabolic
process [PMID:15713680]"
/note="NEDD4 family E3 ubiquitin ligase; regulates
processes including: MVB sorting, the heat shock response,
transcription, endocytosis and ribosome stability;
ubiquitinates Sec23p, Sna3p, Ste4p, Nfi1p, Rpo21p, Sem1p,
Dip5p and Put4p; autoubiquitinates; deubiquitinated by
Ubp2p; regulated by SUMO ligase Siz1p, in turn regulates
Siz1p SUMO ligase activity; required for efficient
Golgi-to-ER trafficking in COPI mutants; mutant tolerates
aneuploidy; human homolog implicated in Liddle syndrome"
/codon_start=1
/product="NEDD4 family E3 ubiquitin-protein ligase"
/protein_id="NP_011051.3"
/db_xref="GeneID:856862"
/db_xref="SGD:S000000927"
/translation="MPSSISVKLVAAESLYKRDVFRSPDPFAVLTIDGYQTKSTSAAK
KTLNPYWNETFKFDDINENSILTIQVFDQKKFKKKDQGFLGVVNVRVGDVLGHLDEDT
ATSSGRPREETITRDLKKSNDGMAVSGRLIVVLSKLPSSSPHSQAPSGHTASSSTNTS
STTRTNGHSTSSTRNHSTSHPSRGTAQAVESTLQSGTTAATNTATTSHRSTNSTSSAT
RQYSSFEDQYGRLPPGWERRTDNFGRTYYVDHNTRTTTWKRPTLDQTEAERGNQLNAN
TELERRQHRGRTLPGGSSDNSSVTVQVGGGSNIPPVNGAAAAAFAATGGTTSGLGELP
SGWEQRFTPEGRAYFVDHNTRTTTWVDPRRQQYIRTYGPTNTTIQQQPVSQLGPLPSG
WEMRLTNTARVYFVDHNTKTTTWDDPRLPSSLDQNVPQYKRDFRRKVIYFRSQPALRI
LPGQCHIKVRRKNIFEDAYQEIMRQTPEDLKKRLMIKFDGEEGLDYGGVSREFFFLLS
HEMFNPFYCLFEYSAYDNYTIQINPNSGINPEHLNYFKFIGRVVGLGVFHRRFLDAFF
VGALYKMMLRKKVVLQDMEGVDAEVYNSLNWMLENSIDGVLDLTFSADDERFGEVVTV
DLKPDGRNIEVTDGNKKEYVELYTQWRIVDRVQEQFKAFMDGFNELIPEDLVTVFDER
ELELLIGGIAEIDIEDWKKHTDYRGYQESDEVIQWFWKCVSEWDNEQRARLLQFTTGT
SRIPVNGFKDLQGSDGPRRFTIEKAGEVQQLPKSHTCFNRVDLPQYVDYDSMKQKLTL
AVEETIGFGQE"
gene complement(<413394..>414179)
/gene="NSA2"
/locus_tag="YER126C"
/db_xref="GeneID:856863"
mRNA complement(<413394..>414179)
/gene="NSA2"
/locus_tag="YER126C"
/product="rRNA-processing protein NSA2"
/transcript_id="NM_001179016.1"
/db_xref="GeneID:856863"
CDS complement(413394..414179)
/gene="NSA2"
/locus_tag="YER126C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10684247]"
/experiment="EXISTENCE:direct assay:GO:0030687
preribosome, large subunit precursor
[PMID:16861225|PMID:17443350|PMID:11583614]"
/experiment="EXISTENCE:mutant phenotype:GO:0000463
maturation of LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16861225]"
/experiment="EXISTENCE:mutant phenotype:GO:0000466
maturation of 5.8S rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16861225]"
/experiment="EXISTENCE:mutant phenotype:GO:0042273
ribosomal large subunit biogenesis [PMID:11583614]"
/note="Protein constituent of 66S pre-ribosomal particles;
contributes to processing of the 27S pre-rRNA; recruited
by ribosomal proteins L17, L35, and L37 to assembling
ribosomes after 27SB pre-rRNA is generated, immediately
preceding removal of ITS2"
/codon_start=1
/product="rRNA-processing protein NSA2"
/protein_id="NP_011052.1"
/db_xref="GeneID:856863"
/db_xref="SGD:S000000928"
/translation="MPQNDYIERHIKQHGKRLDHEERKRKREARESHKISERAQKLTG
WKGKQFAKKRYAEKVSMRKKIKAHEQSKVKGSSKPLDTDGDALPTYLLDREQNNTAKA
ISSSIKQKRLEKADKFSVPLPKVRGISEEEMFKVIKTGKSRSKSWKRMITKHTFVGEG
FTRRPVKMERIIRPSALRQKKANVTHPELGVTVFLPILAVKKNPQSPMYTQLGVLTKG
TIIEVNVSELGMVTAGGKVVWGKYAQVTNEPDRDGCVNAVLLV"
gene <414481..>415554
/gene="LCP5"
/locus_tag="YER127W"
/db_xref="GeneID:856864"
mRNA <414481..>415554
/gene="LCP5"
/locus_tag="YER127W"
/product="small subunit rRNA maturation protein LCP5"
/transcript_id="NM_001179017.1"
/db_xref="GeneID:856864"
CDS 414481..415554
/gene="LCP5"
/locus_tag="YER127W"
/note="Essential protein involved in maturation of 18S
rRNA; depletion leads to inhibited pre-rRNA processing and
reduced polysome levels; localizes primarily to the
nucleolus"
/codon_start=1
/product="small subunit rRNA maturation protein LCP5"
/protein_id="NP_011053.1"
/db_xref="GeneID:856864"
/db_xref="SGD:S000000929"
/translation="MSELNALLKDINGSLTATSESLERLSGIYSNSATDEIPESNQLH
EHLFYDAKKPAEKVSLLSLKNGSMLGYINSLLMLIGNRLDDECKDPSAMDARERSIQH
RVVLERGVKPLEKKLAYQLDKLTRAYVKMEKEYKDAEKRALEKSTLVNHSGNDDSEDD
ESSEDEIAYRPNTSGIINTNKKSSAYRVEETAKQENGEENDDNETGVYKPPKITAVLP
PQQTHFEDRFDAREHKDRSNKSRMQAMEEYIRESSDQPDWSASIGADIVNHGRGGIKS
LRDTEKERRVTSFEEDNFTRLNITNKAEKRKQKQRERNARMNVIGGEDFGIFSSKRKL
EDSTSRRGAKKTRSAWDRAQRRL"
gene <415859..>416470
/gene="VFA1"
/locus_tag="YER128W"
/db_xref="GeneID:856865"
mRNA <415859..>416470
/gene="VFA1"
/locus_tag="YER128W"
/product="Vfa1p"
/transcript_id="NM_001179018.3"
/db_xref="GeneID:856865"
CDS 415859..416470
/gene="VFA1"
/locus_tag="YER128W"
/experiment="EXISTENCE:direct assay:GO:0001671 ATPase
activator activity [PMID:24567329]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:21777356]"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:21777356]"
/experiment="EXISTENCE:mutant phenotype:GO:0007034
vacuolar transport [PMID:21777356]"
/note="Protein that interacts with Vps4p and has a role in
vacuolar sorting; stimulates the ATPase activity of Vps4;
localizes to endosomes in a Vps4-dependent manner;
overexpression causes canavanine sensitivity and confers a
partial class D vacuole morphology"
/codon_start=1
/product="Vfa1p"
/protein_id="NP_011054.3"
/db_xref="GeneID:856865"
/db_xref="SGD:S000000930"
/translation="MINEYVARKVALKDMQPCAICSKPSTTVLYNASGPDWLYTCEIH
LQDNPQFVIPLYSTEYNEAVAQLKLVKGKMDSLTSAQTQLGSWDGWVTKIFSKKEKET
NNSKDPDPTTTDSTDTSPQAKNDAEILSETKKQYSKILDKVTELQRKNRKYELAKIMF
ESRLLRKRTEQVNRERYLKEQENYSNTDPEELLRKHVFPSVPK"
gene <417281..>420709
/gene="SAK1"
/locus_tag="YER129W"
/gene_synonym="PAK1"
/db_xref="GeneID:856866"
mRNA <417281..>420709
/gene="SAK1"
/locus_tag="YER129W"
/gene_synonym="PAK1"
/product="serine/threonine protein kinase SAK1"
/transcript_id="NM_001179019.3"
/db_xref="GeneID:856866"
CDS 417281..420709
/gene="SAK1"
/locus_tag="YER129W"
/gene_synonym="PAK1"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0004674 protein
serine/threonine kinase activity
[PMID:12748292|PMID:12847291|PMID:16201971|PMID:27524664]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:22289182]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0090329
regulation of DNA-templated DNA replication
[PMID:9341678]"
/experiment="EXISTENCE:mutant phenotype:GO:0004674 protein
serine/threonine kinase activity
[PMID:16201971|PMID:12748292]"
/experiment="EXISTENCE:mutant phenotype:GO:0005979
regulation of glycogen biosynthetic process
[PMID:15340085]"
/experiment="EXISTENCE:mutant phenotype:GO:0042149
cellular response to glucose starvation [PMID:15340085]"
/experiment="EXISTENCE:mutant phenotype:GO:1900180
regulation of protein localization to nucleus
[PMID:15340085]"
/experiment="EXISTENCE:mutant phenotype:GO:2000220
regulation of pseudohyphal growth [PMID:19880754]"
/note="Upstream serine/threonine kinase for the SNF1
complex; plays a role in pseudohyphal growth; partially
redundant with Elm1p and Tos3p; members of this family
have functional orthology with LKB1, a mammalian kinase
associated with Peutz-Jeghers cancer-susceptibility
syndrome; SAK1 has a paralog, TOS3, that arose from the
whole genome duplication"
/codon_start=1
/product="serine/threonine protein kinase SAK1"
/protein_id="NP_011055.3"
/db_xref="GeneID:856866"
/db_xref="SGD:S000000931"
/translation="MDRSDKKVNVEEVNVPSNLQIELEKSGTSSSVSLRSPTKSSATN
LAGMAEGARDNASIASSSVDSLNMLLERQRVRQLNHPQHQQHISSSLAKTPTTTSSFC
SSGSSKNKVKETNRISLTYDPVSKRKVLNTYEIIKELGHGQHGKVKLARDILSKQLVA
IKIVDRHEKKQRKFFTFIKSSKISENDKIKREIAIMKKCHHKHVVQLIEVLDDLKSRK
IYLVLEYCSRGEVKWCPPDCMESDAKGPSLLSFQETREILRGVVLGLEYLHYQGIIHR
DIKPANLLISGDGTVKISDFGVSLAASSTNSSDSSESLDELELAKTVGTPAFFAPEMC
LGEDAFTRYNLTKENLFRGSCISFMIDIWAVGVTLYCLLFGMLPFFSDFELKLFEKIV
NDPLKFPTFKEIQSNKVSKVSCEEEYEMAKDLLLKLLEKNPQKRMTIPAIKKHPFVSW
DFDHVPENDEKLLSSVLEQKLRFQCNQTDQFEPISISKHELKNAVSGVGKKIKESVLK
SIPLKDPSDLSNKNYLHPTETTRGRGDANVIVSEGSVLSNIKELSANDGCLNTDSDTN
ININDDDHYSGDDNDGHLTKRELERELNKFDDKHEAGNMVNLPINSSFASLDSFYIDN
FAMARMGMSSPEAGDSVSSVPNLPSAPSSTRLGRSPVFSGVTNQPSPIRPVLPQQKSS
FCATGRYDKSHNSLLRNSSSHLTSYNSGRPSSRTGRMNSRNQNLPKIPNSLSKISTTK
LTELRVPKDSEIPSPAKNPNADRLRRFPVKKNTKTPAIKDPPRININSSDKSGSKNSP
IKSLYQRMKQSKDNSKTFEVRRGNFFSHFNGDDDDSSSQSSVTSSGSESDSELSSTSS
SCTSGTQSRNSSNNNAYSETESLPFEFGVDSEDGSGVLLRDLPNEDQIRPFLDIQPCR
RMKVKSSLNLEPPSVSSSSSSSSDEDELILNVGTAGHRRRHNSSKLSELSNSPQKGSN
NFMYSNGSVHDSETTITPQNMDDLTLHQALSRSQPISKPGPLVLPKRLDQKKATTETS
NLTDIVEFNGNNDHRKDKNFDKVLYSRDLLKDALSSTNAGRRRSIPSNKIRGRKDASI
TMSTNVGNDEHARNTSCHGDKGQENGAIKQRTHERSRSLTVAELNEEKRRSALP"
gene complement(<421115..>422446)
/gene="COM2"
/locus_tag="YER130C"
/db_xref="GeneID:856867"
mRNA complement(<421115..>422446)
/gene="COM2"
/locus_tag="YER130C"
/product="Com2p"
/transcript_id="NM_001179020.1"
/db_xref="GeneID:856867"
CDS complement(421115..422446)
/gene="COM2"
/locus_tag="YER130C"
/experiment="EXISTENCE:direct assay:GO:0003700 DNA-binding
transcription factor activity [PMID:24236068]"
/experiment="EXISTENCE:mutant phenotype:GO:0071252
cellular response to sulfur dioxide [PMID:31799324]"
/experiment="EXISTENCE:mutant phenotype:GO:0080135
regulation of cellular response to stress [PMID:31799324]"
/note="Transcription factor that binds IME1 Upstream
Activation Signal (UAS)ru; COM2 transcription is regulated
by Haa1p, Sok2p and Zap1p transcriptional activators; may
bind the IME1 promoter under all growth conditions to
negatively regulate its transcription in the absence of a
positive regulator that binds more effectively; repressor
activity may depend on phosphorylation by PKA; C. albicans
homolog (MNL1) plays a role in adaptation to stress"
/codon_start=1
/product="Com2p"
/protein_id="NP_011056.1"
/db_xref="GeneID:856867"
/db_xref="SGD:S000000932"
/translation="MSLYPLQRFESNDTVFSYTLNSKTELFNESRNNDKQHFTLQLIP
NANANAKEIDNNNVEIINDLTGNTIVDNCVTTATSSNQLERRLSISDYRTENGNYYEY
EFFGRRELNEPLFNNDIVENDDDIDLNNESDVLMVSDDELEVNERFSFLKQQPLDGLN
RISSTNNLKNLEIHEFIIDPTENIDDELEDSFTTVPQSKKKVRDYFKLNIFGSSSSSN
NNSNSLGCEPIQTENSSSQKMFKNRFFRSRKSTLIKSLPLEQENEVLINSGFDVSSNE
ESDESDHAIINPLKLVGNNKDISTQSIAKTTNPFKSGSDFKMIEPVSKFSNDSRKDLL
AAISEPSSSPSPSAPSPSVQSSSSSHGLVVRKKTGSMQKTRGRKPSLIPDASKQFGCE
FCDRRFKRQEHLKRHVRSLHMCEKPFTCHICNKNFSRSDNLNQHVKTHASL"
gene <423952..>424311
/gene="RPS26B"
/locus_tag="YER131W"
/db_xref="GeneID:856868"
mRNA <423952..>424311
/gene="RPS26B"
/locus_tag="YER131W"
/product="40S ribosomal protein eS26 RPS26B"
/transcript_id="NM_001179021.1"
/db_xref="GeneID:856868"
CDS 423952..424311
/gene="RPS26B"
/locus_tag="YER131W"
/experiment="EXISTENCE:genetic interaction:GO:0000054
ribosomal subunit export from nucleus [PMID:16246728]"
/experiment="EXISTENCE:mutant phenotype:GO:0000463
maturation of LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:25144938]"
/experiment="EXISTENCE:mutant phenotype:GO:0042255
ribosome assembly [PMID:27303706]"
/experiment="EXISTENCE:physical interaction:GO:0030686 90S
preribosome [PMID:25144938]"
/note="Protein component of the small (40S) ribosomal
subunit; homologous to mammalian ribosomal protein S26, no
bacterial homolog; RPS26B has a paralog, RPS26A, that
arose from the whole genome duplication; human homolog can
partially complement an RPS26A, RPS26B double null mutant;
mutations in the human gene are associated with
Diamond-Blackfan anemia"
/codon_start=1
/product="40S ribosomal protein eS26 RPS26B"
/protein_id="NP_011057.1"
/db_xref="GeneID:856868"
/db_xref="SGD:S000000933"
/translation="MPKKRASNGRNKKGRGHVKPVRCVNCSKSIPKDKAIKRMAIRNI
VEAAAVRDLSEASVYPEYALPKTYNKLHYCVSCAIHARIVRVRSREDRKNRAPPQRPR
FNRDNKVSPAAAAKKAL"
gene 424698..424883
/gene="SNR4"
/locus_tag="YNCE0019W"
/db_xref="GeneID:9164882"
ncRNA 424698..424883
/ncRNA_class="snoRNA"
/gene="SNR4"
/locus_tag="YNCE0019W"
/product="SNR4"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:3327689]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:3327689]"
/experiment="EXISTENCE:direct assay:GO:0031428 box C/D
methylation guide snoRNP complex [PMID:10094313]"
/experiment="EXISTENCE:direct assay:GO:0070181 small
ribosomal subunit rRNA binding [PMID:28542199]"
/experiment="EXISTENCE:mutant phenotype:GO:1990882 rRNA
acetylation [PMID:28542199]"
/experiment="EXISTENCE:physical interaction:GO:0031428 box
C/D methylation guide snoRNP complex
[PMID:10733567|PMID:11081632]"
/experiment="EXISTENCE:physical interaction:GO:0042134
rRNA primary transcript binding [PMID:3327689]"
/note="C/D box small nucleolar RNA (snoRNA); acts as
antisense guide to target RNA base acetylation by Kre33p
to C1280 in 18S rRNA (responsible for ac4C1280);
establishes extended bipartite complementarity around
cytosines targeted for acetylation, similar to
pseudouridylation pocket formation by H/ACA snoRNPs; base
pairing between snR4 and 18S rRNA requires putative
helicase activity of Kre33p; deletion increases EtOH
output in industrial strains"
/transcript_id="NR_132169.1"
/db_xref="GeneID:9164882"
/db_xref="SGD:S000006493"
gene complement(<425188..>430449)
/gene="PMD1"
/locus_tag="YER132C"
/db_xref="GeneID:856869"
mRNA complement(<425188..>430449)
/gene="PMD1"
/locus_tag="YER132C"
/product="Pmd1p"
/transcript_id="NM_001179022.3"
/db_xref="GeneID:856869"
CDS complement(425188..430449)
/gene="PMD1"
/locus_tag="YER132C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0030437
ascospore formation [PMID:9383076]"
/experiment="EXISTENCE:mutant phenotype:GO:0030437
ascospore formation [PMID:9383076]"
/note="Protein with an N-terminal kelch-like domain;
putative negative regulator of early meiotic gene
expression; required, with Mds3p, for growth under
alkaline conditions; PMD1 has a paralog, MDS3, that arose
from the whole genome duplication"
/codon_start=1
/product="Pmd1p"
/protein_id="NP_011058.3"
/db_xref="GeneID:856869"
/db_xref="SGD:S000000934"
/translation="MTVLQPPSSVCYPLNLPIVPNPNLDEATRKKLTLECRTGAAVEL
ARSGVFVHGGLTLPLNLTIINSLQLQKELILYFGKQKDRNADFKTLADWISPEIFFLD
LISRTWQRINTTIDTTSENELNNGLSFKERLFHSMCFTESNIYIFGGLMVSPHNGYEL
IATNELWKLDLKTKCWSLISENPQITRRFNHSMHVLNENNENQDTKLIIVGGLDNMDI
PVKKIDIFNLRTSLWESESKSDENPASKGSSKILVNIDGMPISLSHDSNFSVLIENNQ
AEIPTLALYYPQREANTSRRGTDDGSFSTYAHDLDDKSKLPKHHHHHHGDLKYFESDD
ADENAVKTLMSPIVILPLLGNSQGARMTSNPTQNNKENSILQVPFHLQYPSGNYFNYN
IVVIGFYPDPQPSNLHCFIYNIASGKWIRVNIACTECSISMHRFWKLLIWKSHHQALL
LGTRTDDFCSPSVQKFDHILSFSLPMLNGYNKLVNTKHTRTNNGIANSHNLNVNLSLY
DHLPYSNSSTIEHTNPYTVTQGYSLDDSGIPRLTSTATSQFENYSRYITVPLEMESTS
SIFPPYAMVLGKDALEIFGKTLSDFEFITADGDSIGVPVYLLRKRWGRYFDSLLSNGY
ANTSFNYEFNGDTSNIISFSPHTASKTTKFGNSSQSSNGSLEKYFSKNGNSKSNSNTS
LKKPHSVDFTSSTSSPKQRAISHNKLSPSEPILCADEEDSRSNTLKQHATGDTGLKET
GTSNKRPISTTCSSTGMVFRVPFQDMKNSKLGLSEQSGRSTRASSVSPPPVYKKSTND
GNDSNCTLSNTPLVYRRASTVGTTTNSSVDDGFSSIRRASHPLQSYIIAKSSPSSISK
ASPAEKAFSRRKSSALRFIASPNQSRQTSFASTASTASVVSSTSGRRRNSNQISHLGS
SASLPNSPILPVLNIPLPPQEKIPLEPLPPVPKAPSRRSSSLAEYVQFGRDSPVASRR
SSHSTRKSSSSDARRISNSSLLRNTLDSQLLSNSYGSDIPYEASIQEYGMNNGRDEEE
DGDNQDYGCISPSNIRPIFSTINAININGNFKEGEFFSSKSYINNEKSRRLSYISNPE
SVESTNSNNNAIIELEPLLTPRSLYMPWSTASVRAFAEFFYTAQINGKWLLAPVTLDL
LIMAKIYEIPILYELITEVLYKIISKKEEGLSVTCEALLNLFQQKVSRYCNENEGKIR
KQLDSSESYQDTLEIKRSLANIDNGYVDSYLLRNTSMAQSIHYTDDSNGETEIDMHHT
GISSIGSLANRAVPTVFAGGPRDSHNSIGSIAFPSNSGVQNIRRSVSLFSPATKKKSS
LSRETDPLDTSDQFTDDVPDSGPVSRQQNFPRRSSSFTETVPTEPTRYNYQNLDSSKS
NRASDDKEEQNEQATLQDISNFDKYKVETLQKRNSNDGKDLDRTNDPLKNRGTEIPQN
SSNLETDPFIRDSFDSDSGSSFRSDSDDLDSQLGILPFTKMNKKLQEQTSQEFDDSID
PLYKIGSSTPGSSRLHGSFSKYIRPNSQREDGSEYVNISSLENMVSPNALPPVDYVMK
SIYRTSVLVNDSNLMTRTKEAIELSKVLKKLKKKVLQDISQMDDEMRETGKPIFARGS
SSPTLSRQHSDVATPLKQQENTRPALKFASSSPISEGFRKSSIKFSQAPSTQISPRTS
VTDFTASQQRRQHMNKRFSTQTTHSTSALFMNPAFMPSAVNTGRKESEGHCEDRSATA
NRTNRKEDATTNDNDNIAPFPFFGKRR"
gene complement(431129..431220)
/gene="SNR52"
/locus_tag="YNCE0020C"
/db_xref="GeneID:9164886"
ncRNA complement(431129..431220)
/ncRNA_class="snoRNA"
/gene="SNR52"
/locus_tag="YNCE0020C"
/product="SNR52"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:10024243]"
/experiment="EXISTENCE:curator inference:GO:0031428 box
C/D methylation guide snoRNP complex [PMID:10024243]"
/experiment="EXISTENCE:genetic interaction:GO:0030562 rRNA
2'-O-ribose methylation guide activity [PMID:15546625]"
/experiment="EXISTENCE:genetic interaction:GO:0031167 rRNA
methylation [PMID:15546625]"
/experiment="EXISTENCE:mutant phenotype:GO:0030562 rRNA
2'-O-ribose methylation guide activity
[PMID:10024243|PMID:15546625]"
/experiment="EXISTENCE:mutant phenotype:GO:0031167 rRNA
methylation [PMID:10024243|PMID:15546625]"
/note="C/D box small nucleolar RNA (snoRNA); guides
2'-O-methylation of large subunit (LSU) rRNA at position
U2921 and small subunit (SSU) rRNA at position A420"
/transcript_id="NR_132170.1"
/db_xref="GeneID:9164886"
/db_xref="SGD:S000006443"
repeat_region complement(431490..431822)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006895"
gene <432495..>433958
/gene="GLC7"
/locus_tag="YER133W"
/gene_synonym="CID1; DIS2"
/db_xref="GeneID:856870"
mRNA join(<432495..432671,433197..>433958)
/gene="GLC7"
/locus_tag="YER133W"
/gene_synonym="CID1; DIS2"
/product="type 1 serine/threonine-protein phosphatase
catalytic subunit GLC7"
/transcript_id="NM_001179023.3"
/db_xref="GeneID:856870"
CDS join(432495..432671,433197..433958)
/gene="GLC7"
/locus_tag="YER133W"
/gene_synonym="CID1; DIS2"
/EC_number="3.1.3.16"
/experiment="EXISTENCE:direct assay:GO:0000164 protein
phosphatase type 1 complex [PMID:8289829]"
/experiment="EXISTENCE:direct assay:GO:0000776 kinetochore
[PMID:19948764]"
/experiment="EXISTENCE:direct assay:GO:0001400 mating
projection base [PMID:10747092]"
/experiment="EXISTENCE:direct assay:GO:0004722 protein
serine/threonine phosphatase activity [PMID:1660885]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:23418575]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:11412094]"
/experiment="EXISTENCE:direct assay:GO:0005816 spindle
pole body [PMID:10747092]"
/experiment="EXISTENCE:direct assay:GO:0005847 mRNA
cleavage and polyadenylation specificity factor complex
[PMID:12819204]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:11412094]"
/experiment="EXISTENCE:direct assay:GO:0032153 cell
division site [PMID:26771880]"
/experiment="EXISTENCE:genetic interaction:GO:0000076 DNA
replication checkpoint signaling [PMID:19884341]"
/experiment="EXISTENCE:genetic interaction:GO:0000723
telomere maintenance [PMID:29529242]"
/experiment="EXISTENCE:genetic interaction:GO:0008360
regulation of cell shape [PMID:12477789]"
/experiment="EXISTENCE:genetic interaction:GO:0031536
positive regulation of exit from mitosis [PMID:37690059]"
/experiment="EXISTENCE:genetic interaction:GO:2000370
positive regulation of clathrin-dependent endocytosis
[PMID:12356757]"
/experiment="EXISTENCE:mutant phenotype:GO:0000076 DNA
replication checkpoint signaling [PMID:19884341]"
/experiment="EXISTENCE:mutant phenotype:GO:0000077 DNA
damage checkpoint signaling [PMID:19884341]"
/experiment="EXISTENCE:mutant phenotype:GO:0005979
regulation of glycogen biosynthetic process
[PMID:1660885]"
/experiment="EXISTENCE:mutant phenotype:GO:0006873
intracellular monoatomic ion homeostasis [PMID:11973298]"
/experiment="EXISTENCE:mutant phenotype:GO:0006986
response to unfolded protein [PMID:25730376]"
/experiment="EXISTENCE:mutant phenotype:GO:0007059
chromosome segregation [PMID:10072383]"
/experiment="EXISTENCE:mutant phenotype:GO:0007094 mitotic
spindle assembly checkpoint signaling [PMID:10072380]"
/experiment="EXISTENCE:mutant phenotype:GO:0007116
regulation of cell budding [PMID:10639337]"
/experiment="EXISTENCE:mutant phenotype:GO:0007346
regulation of mitotic cell cycle [PMID:23418575]"
/experiment="EXISTENCE:mutant phenotype:GO:0009408
response to heat [PMID:10207049]"
/experiment="EXISTENCE:mutant phenotype:GO:0030437
ascospore formation [PMID:9584086]"
/experiment="EXISTENCE:mutant phenotype:GO:0031297
replication fork processing [PMID:19884341]"
/experiment="EXISTENCE:mutant phenotype:GO:0034501 protein
localization to kinetochore [PMID:19948764]"
/experiment="EXISTENCE:mutant phenotype:GO:0051726
regulation of cell cycle
[PMID:9584086|PMID:7891699|PMID:8164671|PMID:10072380]"
/experiment="EXISTENCE:mutant phenotype:GO:1901901
regulation of protein localization to cell division site
involved in cytokinesis [PMID:26771880]"
/experiment="EXISTENCE:mutant phenotype:GO:1903501
positive regulation of mitotic actomyosin contractile ring
assembly [PMID:26771880]"
/experiment="EXISTENCE:physical interaction:GO:0000164
protein phosphatase type 1 complex [PMID:19948764]"
/experiment="EXISTENCE:physical interaction:GO:0051321
meiotic cell cycle [PMID:8754819]"
/note="Type 1 S/T protein phosphatase (PP1) catalytic
subunit; involved in glycogen metabolism, sporulation and
mitotic progression; interacts with multiple regulatory
subunits; regulates actomyosin ring formation; subunit of
CPF; recruited to mating projections by Afr1p interaction;
regulates nucleocytoplasmic shuttling of Hxk2p; import
into the nucleus is inhibited during spindle assembly
checkpoint arrest; involved in dephosphorylating Rps6a/b
and Bnr1p"
/codon_start=1
/product="type 1 serine/threonine-protein phosphatase
catalytic subunit GLC7"
/protein_id="NP_011059.3"
/db_xref="GeneID:856870"
/db_xref="SGD:S000000935"
/translation="MDSQPVDVDNIIDRLLEVRGSKPGQQVDLEENEIRYLCSKARSI
FIKQPILLELEAPIKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLET
ICLLLAYKIKYPENFFILRGNHECASINRIYGFYDECKRRYNIKLWKTFTDCFNCLPI
AAIIDEKIFCMHGGLSPDLNSMEQIRRVMRPTDIPDVGLLCDLLWSDPDKDIVGWSEN
DRGVSFTFGPDVVNRFLQKQDMELICRAHQVVEDGYEFFSKRQLVTLFSAPNYCGEFD
NAGAMMSVDESLLCSFQILKPAQKSLPRQAGGRKKK"
gene complement(434541..434612)
/locus_tag="YNCE0021C"
/db_xref="GeneID:856871"
tRNA complement(434541..434612)
/locus_tag="YNCE0021C"
/product="tRNA-His"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0030371 translation
repressor activity [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043555 regulation
of translation in response to stress [PMID:27609601]"
/note="Histidine tRNA (tRNA-His), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:856871"
/db_xref="SGD:S000006594"
repeat_region complement(434631..434971)
/note="Ty3 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006906"
repeat_region complement(435128..435447)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006896"
gene complement(435752..435824)
/locus_tag="YNCE0022C"
/db_xref="GeneID:856872"
tRNA complement(435752..435824)
/locus_tag="YNCE0022C"
/product="tRNA-Lys"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:8915539]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:8915539]"
/experiment="EXISTENCE:mutant phenotype:GO:0030533 triplet
codon-amino acid adaptor activity [PMID:17560369]"
/experiment="EXISTENCE:mutant phenotype:GO:0032543
mitochondrial translation [PMID:17560369]"
/note="Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE
analysis; a small portion is imported into mitochondria
via interaction with mt lysyl-tRNA synthetase Msk1p and is
necessary to decode AAG codons at high temperature, when
base modification of mt-encoded tRNA-Lys is reduced"
/db_xref="GeneID:856872"
/db_xref="SGD:S000006619"
repeat_region complement(435946..436277)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006897"
repeat_region complement(436279..436605)
/note="Ty4 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006909"
gene complement(<437267..>437803)
/locus_tag="YER134C"
/db_xref="GeneID:856873"
mRNA complement(<437267..>437803)
/locus_tag="YER134C"
/product="Mg-dependent acid phosphatase"
/transcript_id="NM_001179024.1"
/db_xref="GeneID:856873"
CDS complement(437267..437803)
/locus_tag="YER134C"
/EC_number="3.1.3.48"
/experiment="EXISTENCE:direct assay:GO:0003993 acid
phosphatase activity [PMID:11601995]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0030946 protein
tyrosine phosphatase activity, metal-dependent
[PMID:11601995]"
/note="Magnesium-dependent acid phosphatase; member of the
haloacid dehalogenase superfamily; non-essential gene"
/codon_start=1
/product="Mg-dependent acid phosphatase"
/protein_id="NP_011060.1"
/db_xref="GeneID:856873"
/db_xref="SGD:S000000936"
/translation="MTGYPDVAAFDLDYTIWPCYCDTHLHGPFKPVKSSNGEVLTIIC
RDGYELTIYKDIPRILGDLKDNGVKLMTASRTWAPEIAQEILKIFKVKYAGVVTPLAN
LFDEFQWGERSKIGHLRDGLKDLYNTSDLKSKKICLFDDESRNKEVEKYGVKFVYVRD
PENGPSWKLYQDYLSGKV"
gene complement(<437952..>438344)
/locus_tag="YER135C"
/db_xref="GeneID:856874"
mRNA complement(<437952..>438344)
/locus_tag="YER135C"
/product="uncharacterized protein"
/transcript_id="NM_001348826.1"
/db_xref="GeneID:856874"
CDS complement(437952..438344)
/locus_tag="YER135C"
/note="hypothetical protein; conserved among S. cerevisiae
strains; YER135C is not an essential gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335768.1"
/db_xref="GeneID:856874"
/db_xref="SGD:S000000937"
/translation="MTWTLRNLHNSQKKVLVDITIWLALSPYGDMALFTSFENIIGEY
YRYSILPLHQNLHKLLFTLFLICLASWKEGNRSCRYKWQKFVCNKLNSPETNFVPIRT
SVNKNSKMIVEKQISIHISLKCIVYKEY"
gene 438700..438773
/locus_tag="YNCE0023W"
/db_xref="GeneID:856875"
tRNA 438700..438773
/locus_tag="YNCE0023W"
/product="tRNA-Val"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Valine tRNA (tRNA-Val), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:856875"
/db_xref="SGD:S000006753"
rep_origin 438934..439182
/note="ARS518; Autonomously Replicating Sequence"
/db_xref="SGD:S000077390"
gene <439616..>440971
/gene="GDI1"
/locus_tag="YER136W"
/gene_synonym="SEC19"
/db_xref="GeneID:856876"
mRNA <439616..>440971
/gene="GDI1"
/locus_tag="YER136W"
/gene_synonym="SEC19"
/product="Gdi1p"
/transcript_id="NM_001179026.1"
/db_xref="GeneID:856876"
CDS 439616..440971
/gene="GDI1"
/locus_tag="YER136W"
/gene_synonym="SEC19"
/experiment="EXISTENCE:direct assay:GO:0005093 Rab
GDP-dissociation inhibitor activity [PMID:8157010]"
/experiment="EXISTENCE:mutant phenotype:GO:0016192
vesicle-mediated transport [PMID:8157010]"
/note="GDP dissociation inhibitor; regulates vesicle
traffic in secretory pathways by regulating the
dissociation of GDP from the Sec4/Ypt/rab family of GTP
binding proteins"
/codon_start=1
/product="Gdi1p"
/protein_id="NP_011062.1"
/db_xref="GeneID:856876"
/db_xref="SGD:S000000938"
/translation="MDQETIDTDYDVIVLGTGITECILSGLLSVDGKKVLHIDKQDHY
GGEAASVTLSQLYEKFKQNPISKEERESKFGKDRDWNVDLIPKFLMANGELTNILIHT
DVTRYVDFKQVSGSYVFKQGKIYKVPANEIEAISSPLMGIFEKRRMKKFLEWISSYKE
DDLSTHQGLDLDKNTMDEVYYKFGLGNSTKEFIGHAMALWTNDDYLQQPARPSFERIL
LYCQSVARYGKSPYLYPMYGLGELPQGFARLSAIYGGTYMLDTPIDEVLYKKDTGKFE
GVKTKLGTFKAPLVIADPTYFPEKCKSTGQRVIRAICILNHPVPNTSNADSLQIIIPQ
SQLGRKSDIYVAIVSDAHNVCSKGHYLAIISTIIETDKPHIELEPAFKLLGPIEEKFM
GIAELFEPREDGSKDNIYLSRSYDASSHFESMTDDVKDIYFRVTGHPLVLKQRQEQEK
Q"
gene complement(<441373..>441819)
/locus_tag="YER137C"
/db_xref="GeneID:856877"
mRNA complement(<441373..>441819)
/locus_tag="YER137C"
/product="uncharacterized protein"
/transcript_id="NM_001179027.3"
/db_xref="GeneID:856877"
CDS complement(441373..441819)
/locus_tag="YER137C"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011063.3"
/db_xref="GeneID:856877"
/db_xref="SGD:S000000939"
/translation="MCESSNKTENDIVRLSQAMDVLAKLIISKQKDGSQLQVEYEHKL
KELEKFINLLLGLHESTVGSMMNTSVLDMVLRNGIEIMEKDDQKYALIPIKAKEEADK
TTSTIQGVTSKKSSKKKKNKIKCSFCHEAGHTRAHCGARLTVIPKK"
gene 441987..442508
/gene="SCR1"
/locus_tag="YNCE0024W"
/db_xref="GeneID:9164887"
ncRNA 441987..442508
/ncRNA_class="SRP_RNA"
/gene="SCR1"
/locus_tag="YNCE0024W"
/product="SCR1"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:18418087]"
/experiment="EXISTENCE:direct assay:GO:0005786 signal
recognition particle, endoplasmic reticulum targeting
[PMID:7925282]"
/experiment="EXISTENCE:direct assay:GO:0006614
SRP-dependent cotranslational protein targeting to
membrane [PMID:10921896]"
/experiment="EXISTENCE:mutant phenotype:GO:0006614
SRP-dependent cotranslational protein targeting to
membrane [PMID:1655273]"
/experiment="EXISTENCE:mutant phenotype:GO:0045047 protein
targeting to ER [PMID:7925282]"
/note="RNA subunit of the Signal Recognition Particle
(SRP)"
/transcript_id="NR_132171.1"
/db_xref="GeneID:9164887"
/db_xref="SGD:S000006491"
rep_origin 442416..442735
/note="ARS519; Autonomously Replicating Sequence"
/db_xref="SGD:S000077391"
repeat_region 442734..443072
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006917"
gene complement(443202..443275)
/locus_tag="YNCE0025C"
/db_xref="GeneID:856878"
tRNA complement(443202..443275)
/locus_tag="YNCE0025C"
/product="tRNA-Ile"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Isoleucine tRNA (tRNA-Ile), predicted by
tRNAscan-SE analysis"
/db_xref="GeneID:856878"
/db_xref="SGD:S000006602"
mobile_element complement(443397..449320)
/note="YERCTy1-1; Ty1 element, LTR retrotransposon of the
Copia (Pseudoviridae) group; contains co-transcribed genes
TYA Gag and TYB Pol, encoding proteins involved in
structure and function of virus-like particles, flanked by
two direct repeats"
/mobile_element_type="retrotransposon:YERCTy1-1"
/db_xref="SGD:S000006910"
repeat_region complement(443397..443733)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006898"
gene complement(<443756..>449024)
/locus_tag="YER138C"
/db_xref="GeneID:856879"
mRNA complement(<443756..>449024)
/locus_tag="YER138C"
/product="gag-pol fusion protein"
/transcript_id="NM_001179028.4"
/db_xref="GeneID:856879"
CDS complement(join(443756..447721,447723..449024))
/locus_tag="YER138C"
/EC_number="2.7.7.7"
/EC_number="2.7.7.49"
/EC_number="3.1.26.4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9448009]"
/ribosomal_slippage
/note="Retrotransposon TYA Gag and TYB Pol genes;
transcribed/translated as one unit; polyprotein is
processed to make a nucleocapsid-like protein (Gag),
reverse transcriptase (RT), protease (PR), and integrase
(IN); similar to retroviral genes"
/codon_start=1
/product="gag-pol fusion protein"
/protein_id="NP_011064.3"
/db_xref="GeneID:856879"
/db_xref="SGD:S000000940"
/translation="MESQQLSQHSPISHGSACASVTSKEVHTNQDPLDVSASKTEECE
KASTKANSQQTTTPASSAVPENPHHASPQPASVPPPQNGPYPQQCMMTQNQANPSGWS
FYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVRQITDDELT
FLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQ
YNGSTPADAFETKVTNIIDRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNM
TVAELFLDIHAIYEEQQGSRNSKPNYRRNLSDEKNDSRSYTNTTKPKVIARNPQKTNN
SKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLGQELTESTVNHTNHS
DDELPGHLLLDSGASRTLIRSAHHIHSASSNPDINVVDAQKRNIPINAIGDLQFHFQD
NTKTSIKVLHTPNIAYDLLSLNELAAVDITACFTKNVLERSDGTVLAPIVKYGDFYWV
SKKYLLPSNISVPTINNVHTSESTRKYPYPFIHRMLAHANAQTIRYSLKNNTITYFNE
SDVDWSSAIDYQCPDCLIGKSTKHRHIKGSRLKYQNSYEPFQYLHTDIFGPVHNLPKS
APSYFISFTDETTKFRWVYPLHDRREDSILDVFTTILAFIKNQFQASVLVIQMDRGSE
YTNRTLHKFLEKNGITPCYTTTADSRAHGVAERLNRTLLDDCRTQLQCSGLPNHLWFS
AIEFSTIVRNSLASPKSKKSARQHAGLAGLDISTLLPFGQPVIVNDHNPNSKIHPRGI
PGYALHPSRNSYGYIIYLPSLKKTVDTTNYVILQGKESRLDQFNYDALTFDEDLNRLT
ASYQSFIASNEIQQSDDLNIESDHDFQSDIELHPEQPRNVLSKAVSPTDSTPPSTHTE
DSKRVSKTNIRAPREVDPNISESNILPSKKRSSTPQISNIESTGSGGMHKLNVPLLAP
MSQSNTHESSHASKSKDFRHSDSYSENETNHTNVPISSTGGTNNKTVPQISDQETEKR
IIHRSPSIDASPPENNSSHNIVPIKTPTTVSEQNTEESIIADLPLPDLPPESPTEFPD
PFKELPPINSHQTNSSLGGIGDSNAYTTINSKKRSLEDNETEIKVSRDTWNTKNMRSL
EPPRSKKRIHLIAAVKAVKSIKPIRTTLRYDEAITYNKDIKEKEKYIEAYHKEVNQLL
KMKTWDTDEYYDRKEIDPKRVINSMFIFNKKRDGTHKARFVARGDIQHPDTYDSGMQS
NTVHHYALMTSLSLALDNNYYITQLDISSAYLYADIKEELYIRPPPHLGMNDKLIRLK
KSLYGLKQSGANWYETIKSYLIKQCGMEEVRGWSCVFKNSQVTICLFVDDMILFSKDL
NANKKIITTLKKQYDTKIINLGESDNEIQYDILGLEIKYQRGKYMKLGMENSLTEKIP
KLNVPLNPKGRKLSAPGQPGLYIDQDELEIDEDEYKEKVHEMQKLIGLASYVGYKFRF
DLLYYINTLAQHILFPSRQVLDMTYELIQFMWDTRDKQLIWHKNKPTEPDNKLVAISD
ASYGNQPYYKSQIGNIYLLNGKVIGGKSTKASLTCTSTTEAEIHAISESVPLLNNLSY
LIQELNKKPIIKGLLTDSRSTISIIKSTNEEKFRNRFFGTKAMRLRDEVSGNNLYVYY
IETKKNIADVMTKPLPIKTFKLLTNKWIH"
repeat_region 444834..445139
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006919"
gene complement(<447702..>449024)
/locus_tag="YER137C-A"
/db_xref="GeneID:856880"
mRNA complement(<447702..>449024)
/locus_tag="YER137C-A"
/product="gag protein"
/transcript_id="NM_001184428.1"
/db_xref="GeneID:856880"
CDS complement(447702..449024)
/locus_tag="YER137C-A"
/note="Retrotransposon TYA Gag gene co-transcribed with
TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is
a nucleocapsid protein that is the structural constituent
of virus-like particles (VLPs); similar to retroviral Gag"
/codon_start=1
/product="gag protein"
/protein_id="NP_058155.1"
/db_xref="GeneID:856880"
/db_xref="SGD:S000007402"
/translation="MESQQLSQHSPISHGSACASVTSKEVHTNQDPLDVSASKTEECE
KASTKANSQQTTTPASSAVPENPHHASPQPASVPPPQNGPYPQQCMMTQNQANPSGWS
FYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVRQITDDELT
FLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQ
YNGSTPADAFETKVTNIIDRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNM
TVAELFLDIHAIYEEQQGSRNSKPNYRRNLSDEKNDSRSYTNTTKPKVIARNPQKTNN
SKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLRPGTY"
repeat_region complement(448984..449320)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006899"
repeat_region 449274..449626
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000303805"
gene <449474..>449578
/locus_tag="YER138W-A"
/db_xref="GeneID:856881"
mRNA <449474..>449578
/locus_tag="YER138W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184332.1"
/db_xref="GeneID:856881"
CDS 449474..449578
/locus_tag="YER138W-A"
/note="hypothetical protein; YER138W-A has a paralog,
YBL107W-A, that arose from a single-locus duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011065.1"
/db_xref="GeneID:856881"
/db_xref="SGD:S000007239"
/translation="MIIIFIELCRIADSLLWIPKSSRRTSSISTYLIL"
gene complement(<450563..>451243)
/gene="RTR1"
/locus_tag="YER139C"
/db_xref="GeneID:856882"
mRNA complement(<450563..>451243)
/gene="RTR1"
/locus_tag="YER139C"
/product="RNA polymerase II subunit B1 CTD phosphatase
RTR1"
/transcript_id="NM_001179029.3"
/db_xref="GeneID:856882"
CDS complement(450563..451243)
/gene="RTR1"
/locus_tag="YER139C"
/EC_number="3.1.3.16"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:18408053]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:18408053]"
/experiment="EXISTENCE:direct assay:GO:0008420 RNA
polymerase II CTD heptapeptide repeat phosphatase activity
[PMID:19394294]"
/experiment="EXISTENCE:genetic interaction:GO:0006366
transcription by RNA polymerase II [PMID:18408053]"
/experiment="EXISTENCE:mutant phenotype:GO:0006366
transcription by RNA polymerase II [PMID:18408053]"
/experiment="EXISTENCE:physical interaction:GO:0006366
transcription by RNA polymerase II [PMID:18408053]"
/note="Dual specificity protein phosphatase;
dephosphorylates T1 and S5 in C-terminal domain (CTD) of
RNA polymerase II largest subunit, Rpo21p; autoregulates
turnover of its mRNA; has a cysteine-rich motif required
for function and conserved in eukaryotes; shuttles between
the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that
arose from the whole genome duplication"
/codon_start=1
/product="RNA polymerase II subunit B1 CTD phosphatase
RTR1"
/protein_id="NP_011066.3"
/db_xref="GeneID:856882"
/db_xref="SGD:S000000941"
/translation="MATIEDIKETALIPFQKHRQLSMHEAEVITLEIIGLLCDSECKD
EKTLKYLGRFLTPDMYQDLVDERNLNKRCGYPLCGKSPERIRDPFSMNDTTKKFLLEN
NPYAYLSHYCSKFHFRCSQFYQVQLSDEALFARTGVHLFEDPEQDKHDIDFKVTLFEE
LLREKASEEDIKSLISGLKKLGLNPDSGTTEKDDTELEDDLSKWLAQIKIVENDNPSI
LGDFTRED"
gene <451565..>453235
/gene="EMP65"
/locus_tag="YER140W"
/db_xref="GeneID:856883"
mRNA <451565..>453235
/gene="EMP65"
/locus_tag="YER140W"
/product="Emp65p"
/transcript_id="NM_001179030.3"
/db_xref="GeneID:856883"
CDS 451565..453235
/gene="EMP65"
/locus_tag="YER140W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:23275891]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0034975
protein folding in endoplasmic reticulum [PMID:19325107]"
/note="Integral membrane protein of the ER; forms an
ER-membrane associated protein complex with Slp1p;
identified along with SLP1 in a screen for mutants
defective in the unfolded protein response (UPR); proposed
to function in the folding of integral membrane proteins;
interacts genetically with MPS3; the authentic, non-tagged
protein is detected in highly purified mitochondria in
high-throughput studies"
/codon_start=1
/product="Emp65p"
/protein_id="NP_011067.3"
/db_xref="GeneID:856883"
/db_xref="SGD:S000000942"
/translation="MQHKDTAVAKDTAKKRLLRRNSAPSAIHIISRLDKKWSFLWNTI
DRHNIVEEQDESSAAKSEEEHEDDYELEQLLNMIRIPMFLEKFMLFALLTSLDCFLYY
FTVLPIRLIKGYVKQFKSYRQHYRLQQRSGHKNKIPFRYRITSREYKERCMIFIIVIS
SILLSKLDTSKLYHRIKRQSTMKLYMLFSVLEMADKMLASLGQSLLTVMLSRKNSERI
LLHKCLLVSMSLTYVTIHGYVLVYQAISLNIAVNSYSNALLTLLLSMQFAEIKSSVLK
KFDKEGFFQITIADVVERFKLTLLLSITGLRNLQSWSSSLSNTSINFWSPRSTLSIVI
NILCGPMVSVVGSEVLVDWAKHAYITKFNRIRPQIYDKFYYIIYKDYSTRTHKLEDRL
GLPLPAFVVLFIVMVRPTLFKSSEPSYLPSLFRILFMGASVFLLALLAKFTLDLILIK
WSKRIEQRFRDQAFNTVVTEEEYVPGLLSGGMGKVDVSTRIALHSDYNKENRIETESV
SPMRKRKTTLTAECTPPSLNDIRRQKDSKNPRSLENVARYKMVSKRIW"
gene <453459..>454919
/gene="COX15"
/locus_tag="YER141W"
/db_xref="GeneID:856884"
mRNA <453459..>454919
/gene="COX15"
/locus_tag="YER141W"
/product="Cox15p"
/transcript_id="NM_001179031.1"
/db_xref="GeneID:856884"
CDS 453459..454919
/gene="COX15"
/locus_tag="YER141W"
/EC_number="1.17.99.9"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:9228094]"
/experiment="EXISTENCE:genetic interaction:GO:0006784 heme
A biosynthetic process [PMID:11788607]"
/experiment="EXISTENCE:genetic interaction:GO:0120547 heme
A synthase activity [PMID:11788607]"
/experiment="EXISTENCE:mutant phenotype:GO:0006784 heme A
biosynthetic process [PMID:11788607]"
/experiment="EXISTENCE:mutant phenotype:GO:0120547 heme A
synthase activity [PMID:26940873|PMID:11248251]"
/note="Heme a synthase; required for the hydroxylation of
heme O to form heme A, an essential prosthetic group for
cytochrome c oxidase; oligomerization is required for
function"
/codon_start=1
/product="Cox15p"
/protein_id="NP_011068.1"
/db_xref="GeneID:856884"
/db_xref="SGD:S000000943"
/translation="MLFRNIEVGRQAAKLLTRTSSRLAWQSIGASRNISTIRQQIRKT
QLYNFKKTVSIRPFSLSSPVFKPHVASESNPIESRLKTSKNVAYWLIGTSGLVFGIVV
LGGLTRLTESGLSITEWKPVTGTLPPMNQKEWEEEFIKYKESPEFKLLNSHIDLDEFK
FIFFMEWIHRLWGRAIGAVFILPAVYFAVSKKTSGHVNKRLFGLAGLLGLQGFVGWWM
VKSGLDQEQLDARKSKPTVSQYRLTTHLGTAFFLYMGMLWTGLEILRECKWIKNPVQA
ISLFKKLDNPAIGPMRKISLALLAVSFLTAMSGGMVAGLDAGWVYNTWPKMGERWFPS
SRELMDENFCRREDKKDLWWRNLLENPVTVQLVHRTCAYVAFTSVLAAHMYAIKKKAV
IPRNAMTSLHVMMGVVTLQATLGILTILYLVPISLASIHQAGALALLTSSLVFASQLR
KPRAPMRNVIITLPHSSKVTSGKILSEASKLASKPL"
gene complement(<455146..>456036)
/gene="MAG1"
/locus_tag="YER142C"
/gene_synonym="MMS5"
/db_xref="GeneID:856885"
mRNA complement(<455146..>456036)
/gene="MAG1"
/locus_tag="YER142C"
/gene_synonym="MMS5"
/product="DNA-3-methyladenine glycosylase II"
/transcript_id="NM_001179032.1"
/db_xref="GeneID:856885"
CDS complement(455146..456036)
/gene="MAG1"
/locus_tag="YER142C"
/gene_synonym="MMS5"
/EC_number="3.2.2.21"
/experiment="EXISTENCE:curator inference:GO:0005634
nucleus [PMID:2265619]"
/experiment="EXISTENCE:direct assay:GO:0003684 damaged DNA
binding [PMID:18472311]"
/experiment="EXISTENCE:direct assay:GO:0003905 alkylbase
DNA N-glycosylase activity [PMID:2682633|PMID:2265619]"
/experiment="EXISTENCE:direct assay:GO:0008725
DNA-3-methyladenine glycosylase activity [PMID:18472311]"
/experiment="EXISTENCE:genetic interaction:GO:0006285
base-excision repair, AP site formation [PMID:18043759]"
/experiment="EXISTENCE:mutant phenotype:GO:0006285
base-excision repair, AP site formation [PMID:18043759]"
/experiment="EXISTENCE:mutant phenotype:GO:0006307 DNA
alkylation repair [PMID:2265620]"
/note="3-methyl-adenine DNA glycosylase; initiates base
excision repair (BER) by removing damaged bases to create
abasic sites that are subsequently repaired; expansion of
BER repair removes alkylated bases resulting from
alkylating agent-induced DNA damage, compensating for the
lack of DNA repair by oxidative dealkylation; protein
abundance increases in response to DNA replication stress"
/codon_start=1
/product="DNA-3-methyladenine glycosylase II"
/protein_id="NP_011069.1"
/db_xref="GeneID:856885"
/db_xref="SGD:S000000944"
/translation="MKLKREYDELIKADAVKEIAKELGSRPLEVALPEKYIARHEEKF
NMACEHILEKDPSLFPILKNNEFTLYLKETQVPNTLEDYFIRLASTILSQQISGQAAE
SIKARVVSLYGGAFPDYKILFEDFKDPAKCAEIAKCGLSKRKMIYLESLAVYFTEKYK
DIEKLFGQKDNDEEVIESLVTNVKGIGPWSAKMFLISGLKRMDVFAPEDLGIARGFSK
YLSDKPELEKELMRERKVVKKSKIKHKKYNWKIYDDDIMEKCSETFSPYRSVFMFILW
RLASTNTDAMMKAEENFVKS"
gene <456319..>457605
/gene="DDI1"
/locus_tag="YER143W"
/gene_synonym="VSM1"
/db_xref="GeneID:856886"
mRNA <456319..>457605
/gene="DDI1"
/locus_tag="YER143W"
/gene_synonym="VSM1"
/product="Ddi1p"
/transcript_id="NM_001179033.3"
/db_xref="GeneID:856886"
CDS 456319..457605
/gene="DDI1"
/locus_tag="YER143W"
/gene_synonym="VSM1"
/experiment="EXISTENCE:direct assay:GO:0000149 SNARE
binding [PMID:10330187]"
/experiment="EXISTENCE:direct assay:GO:0004190
aspartic-type endopeptidase activity [PMID:32193351]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:10330187]"
/experiment="EXISTENCE:direct assay:GO:0030674
protein-macromolecule adaptor activity [PMID:15964793]"
/experiment="EXISTENCE:direct assay:GO:0031593
polyubiquitin modification-dependent protein binding
[PMID:32193351]"
/experiment="EXISTENCE:direct assay:GO:0043130 ubiquitin
binding [PMID:11323716]"
/experiment="EXISTENCE:direct assay:GO:1904855 proteasome
regulatory particle binding [PMID:15964793]"
/experiment="EXISTENCE:genetic interaction:GO:0043328
protein transport to vacuole involved in
ubiquitin-dependent protein catabolic process via the
multivesicular body sorting pathway [PMID:29444958]"
/experiment="EXISTENCE:mutant phenotype:GO:0004190
aspartic-type endopeptidase activity [PMID:32193351]"
/experiment="EXISTENCE:mutant phenotype:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:12051757]"
/experiment="EXISTENCE:mutant phenotype:GO:0009306 protein
secretion [PMID:21094643]"
/experiment="EXISTENCE:mutant phenotype:GO:0016192
vesicle-mediated transport [PMID:10330187]"
/experiment="EXISTENCE:mutant phenotype:GO:0031593
polyubiquitin modification-dependent protein binding
[PMID:32193351]"
/experiment="EXISTENCE:mutant phenotype:GO:0043328 protein
transport to vacuole involved in ubiquitin-dependent
protein catabolic process via the multivesicular body
sorting pathway [PMID:29444958]"
/experiment="EXISTENCE:mutant phenotype:GO:0045740
positive regulation of DNA replication [PMID:31276951]"
/note="Aspartic-type endopeptidase; involved in the
cleavage of long-chain polyubiquitinated substrates; role
in proteasome-independent, ubiquitin (Ub)-dependent
proteolysis and DNA-protein crosslink repair; Ub-shuttle
receptor for HO and Ufo1p; DNA damage-inducible v-SNARE
binding protein and negative regulator of constitutive
exocytosis; possible role in the S-phase checkpoint;
UBL-UBA family member containing Ub-like (UBL), helical,
Ub-associated (UBA) and retroviral-like proteinase (RVP)
domains"
/codon_start=1
/product="Ddi1p"
/protein_id="NP_011070.3"
/db_xref="GeneID:856886"
/db_xref="SGD:S000000945"
/translation="MDLTISNELTGEIYGPIEVSEDMALTDLIALLQADCGFDKTKHD
LYYNMDILDSNRTQSLKELGLKTDDLLLIRGKISNSIQTDAATLSDEAFIEQFRQELL
NNQMLRSQLILQIPGLNDLVNDPLLFRERLGPLILQRRYGGYNTAMNPFGIPQDEYTR
LMANPDDPDNKKRIAELLDQQAIDEQLRNAIEYTPEMFTQVPMLYINIEINNYPVKAF
VDTGAQTTIMSTRLAKKTGLSRMIDKRFIGEARGVGTGKIIGRIHQAQVKIETQYIPC
SFTVLDTDIDVLIGLDMLKRHLACVDLKENVLRIAEVETSFLSEAEIPKSFQEGLPAP
TSVTTSSDKPLTPTKTSSTLPPQPGAVPALAPRTGMGPTPTGRSTAGATTATGRTFPE
QTIKQLMDLGFPRDAVVKALKQTNGNAEFAASLLFQ"
gene complement(<457806..>460223)
/gene="UBP5"
/locus_tag="YER144C"
/db_xref="GeneID:856887"
mRNA complement(<457806..>460223)
/gene="UBP5"
/locus_tag="YER144C"
/product="putative ubiquitin-specific protease UBP5"
/transcript_id="NM_001179034.3"
/db_xref="GeneID:856887"
CDS complement(457806..460223)
/gene="UBP5"
/locus_tag="YER144C"
/EC_number="3.4.19.12"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:17145966]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:17145966]"
/note="Putative ubiquitin-specific protease; concentrates
at the bud neck; UBP5 has a paralog, DOA4, that arose from
the whole genome duplication"
/codon_start=1
/product="putative ubiquitin-specific protease UBP5"
/protein_id="NP_011071.3"
/db_xref="GeneID:856887"
/db_xref="SGD:S000000946"
/translation="MGSEQALSEVVESAKERFGRLRHLVQKFLDDDDVPQECLPLLQE
CAEIWSSYVDACQDITMQAPKEDANRLSKGFLRLNETAFLYYMIVYTLLEDTLPRLKE
FSSNKDQNVRNLYGERIQLLHNDPNIERIRNVIENYPKFIQLQTIEPGKLSSMLHFHG
DALLLIDVRPRSEFVRAHIKCKNIICIDPASFKDSFTDQQIESVSLITSPHSDITFFS
NRDKFKFIILYTDTQLHNNFQQRQTRILAKILSQNSVIKPLSGTKILILENGFSNWVK
LGGAYQSSVSETAHLTSSSSTPAFGSPQVPTGLFNQKSLSPNKDKSMPMVSMNTQPLL
TTVQRPQLPLYYSDLPIIPQPSPNRNSPTVQKFSPHPPTTLSKLNTPSTIQNKANTVE
RISPDIRAAQAHAYLPPASNVFSPRIPPLPQQNLSSSRQTILNNSQVLDLDLIVGLEN
IGNCCYMNCILQCLVGTHDLVRMFLDNTYLNFINFDSSRGSKGLLAKNFAILVNNMHR
HGAFTPPNVRTIPVQTIQFKKICGHINPMYSDSMQQDCQEFCQFLLDGLHEDLNQNGS
KKHLKQLSDEEERMREKMSIRKASALEWERFLLTDFSAIIDLFQGQYASRLQCQVCEH
TSTTYQTFSVLSVPVPRVKTCNILDCFREFTKCERLGVDEQWSCPKCLKKQPSTKQLK
ITRLPKKLIINLKRFDNQMNKNNVFVQYPYSLDLTPYWARDFNHEAIVNEDIPTRGQV
PPFRYRLYGVACHSGSLYGGHYTSYVYKGPKKGWYFFDDSLYRPITFSTEFITPSAYV
LFYERIF"
gene complement(<460526..>461740)
/gene="FTR1"
/locus_tag="YER145C"
/db_xref="GeneID:856888"
mRNA complement(<460526..>461740)
/gene="FTR1"
/locus_tag="YER145C"
/product="high-affinity iron permease FTR1"
/transcript_id="NM_001179035.1"
/db_xref="GeneID:856888"
CDS complement(460526..461740)
/gene="FTR1"
/locus_tag="YER145C"
/experiment="EXISTENCE:direct assay:GO:0005381 iron ion
transmembrane transporter activity
[PMID:16700543|PMID:8599111]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:10748025|PMID:16700543]"
/experiment="EXISTENCE:direct assay:GO:0033573
high-affinity iron permease complex [PMID:16522632]"
/experiment="EXISTENCE:genetic interaction:GO:0034755 iron
ion transmembrane transport [PMID:8599111]"
/experiment="EXISTENCE:genetic interaction:GO:1901684
arsenate ion transmembrane transport [PMID:23295455]"
/experiment="EXISTENCE:mutant phenotype:GO:0034755 iron
ion transmembrane transport [PMID:16700543|PMID:8599111]"
/note="High affinity iron permease; involved in the
transport of iron across the plasma membrane; forms
complex with Fet3p; expression is regulated by iron;
protein abundance increases in response to DNA replication
stress"
/codon_start=1
/product="high-affinity iron permease FTR1"
/protein_id="NP_011072.1"
/db_xref="GeneID:856888"
/db_xref="SGD:S000000947"
/translation="MPNKVFNVAVFFVVFRECLEAVIVISVLLSFLKQAIGEHDRALY
RKLRIQVWVGVLLGFIICLAIGAGFIGAYYSLQKDIFGSAEDLWEGIFCMIATIMISM
MGIPMLRMNKMQSKWRVKIARSLVEIPHRKRDYFKIGFLSRRYAMFLLPFITVLREGL
EAVVFVAGAGITTQGSHASAYPLPVVVGLICGGLVGYLLYYGASKSSLQIFLILSTSI
LYLISAGLFSRGAWYFENYRFNLASGGDASEGGDGNGSYNIRKAVYHVNCCNPELDNG
WDIFNALLGWQNTGYLSSMLCYNIYWLVLIIVLSLMIFEERRGHLPFTKNLQLKHLNP
GYWIKNKKKQELTEEQKRQLFAKMENINFNEDGEINVQENYELPEQTTSHSSSQNVAT
DKEVLHVKADSL"
gene complement(<462386..>462823)
/locus_tag="YER145C-A"
/db_xref="GeneID:31009439"
mRNA complement(<462386..>462823)
/locus_tag="YER145C-A"
/product="uncharacterized protein"
/transcript_id="NM_001348827.1"
/db_xref="GeneID:31009439"
CDS complement(462386..462823)
/locus_tag="YER145C-A"
/note="hypothetical protein; conserved among S. cerevisiae
strains; overlaps verified ORF LSM5/YER146W"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335769.1"
/db_xref="GeneID:31009439"
/db_xref="SGD:S000028758"
/translation="MAILLPLKSILPWCCITFSFLLSSSGSISHSTASSSITLTKSSK
PTNVPSNSRFDCSTINTFWLIVLSMTSKGKISGRLILRASVYACECTCIRYACCETIY
PPRKPFSLSLYFFYFNKKASILFCYPDAKTKPEHPGNKRAGSG"
gene <462585..>462866
/gene="LSM5"
/locus_tag="YER146W"
/db_xref="GeneID:856889"
mRNA <462585..>462866
/gene="LSM5"
/locus_tag="YER146W"
/product="RNA-binding protein LSM5"
/transcript_id="NM_001179036.1"
/db_xref="GeneID:856889"
CDS 462585..462866
/gene="LSM5"
/locus_tag="YER146W"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:15075370|PMID:10369684]"
/experiment="EXISTENCE:direct assay:GO:0005688 U6 snRNP
[PMID:11720284|PMID:10369684]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:15075370]"
/experiment="EXISTENCE:direct assay:GO:0046540 U4/U6 x U5
tri-snRNP complex [PMID:10449419|PMID:10377396]"
/experiment="EXISTENCE:direct assay:GO:1990726 Lsm1-7-Pat1
complex [PMID:24139796]"
/experiment="EXISTENCE:mutant phenotype:GO:0000398 mRNA
splicing, via spliceosome [PMID:10428970]"
/experiment="EXISTENCE:physical interaction:GO:0005732
sno(s)RNA-containing ribonucleoprotein complex
[PMID:15075370]"
/note="Lsm (Like Sm) protein; part of heteroheptameric
complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic
Lsm1p complex involved in mRNA decay; nuclear Lsm8p
complex part of U6 snRNP and possibly involved in
processing tRNA, snoRNA, and rRNA"
/codon_start=1
/product="RNA-binding protein LSM5"
/protein_id="NP_011073.1"
/db_xref="GeneID:856889"
/db_xref="SGD:S000000948"
/translation="MSLPEILPLEVIDKTINQKVLIVLQSNREFEGTLVGFDDFVNVI
LEDAVEWLIDPEDESRNEKVMQHHGRMLLSGNNIAILVPGGKKTPTEAL"
gene complement(<462968..>464842)
/gene="SCC4"
/locus_tag="YER147C"
/db_xref="GeneID:856890"
mRNA complement(<462968..>464842)
/gene="SCC4"
/locus_tag="YER147C"
/product="cohesin-loading factor complex subunit SCC4"
/transcript_id="NM_001179037.3"
/db_xref="GeneID:856890"
CDS complement(462968..464842)
/gene="SCC4"
/locus_tag="YER147C"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:10882066]"
/experiment="EXISTENCE:direct assay:GO:0043515 kinetochore
binding [PMID:28938124]"
/experiment="EXISTENCE:mutant phenotype:GO:0006302
double-strand break repair [PMID:15610742]"
/experiment="EXISTENCE:mutant phenotype:GO:0007064 mitotic
sister chromatid cohesion [PMID:10882066]"
/experiment="EXISTENCE:mutant phenotype:GO:0007076 mitotic
chromosome condensation [PMID:18708580]"
/experiment="EXISTENCE:mutant phenotype:GO:0070550 rDNA
chromatin condensation [PMID:18708580]"
/experiment="EXISTENCE:mutant phenotype:GO:0071169
establishment of protein localization to chromatin
[PMID:10882066]"
/experiment="EXISTENCE:physical interaction:GO:0032116 SMC
loading complex [PMID:10882066]"
/note="Subunit of cohesin loading factor (Scc2p-Scc4p);
complex is required for the loading of cohesin complexes
onto chromosomes; involved in establishing sister
chromatid cohesion during double-strand break repair via
phosphorylated histone H2AX"
/codon_start=1
/product="cohesin-loading factor complex subunit SCC4"
/protein_id="NP_011074.3"
/db_xref="GeneID:856890"
/db_xref="SGD:S000000949"
/translation="MENLGDKLSISQVYHLAQEYRDHAYSIANKIGSEEGLKQYYGLM
NMSIQMFQLLKTKCTLSVLEDSKVTFEMVELLIQETYNFDLAELYISSLKERLQTHQS
DTDLVEEIMRCEFLLLHDLPLMRDSKFHYKIALRNCNELVQYMVNLQDELYQNWASVF
QYVGVMLCIKLKQHRRVKTSFHGLLSQCREKSQWKWFLNLCYVNYLLNERFPIPEDAL
QELRSTELHTVGPELYAWKLALEMVIQLCKDGNITDHLNEFKNFFDTNKQSLVTNEGK
GCVIKIMPRIALKVELPMIFHYKELKNILLLLQSVSYIVNCYDEKGNFSRKFLPKVYS
TTQKLIKNIAAGGVSMNELDSRIQTYKSILEFCEFYKVWEQTLLKGAVVTTESPKLGP
SPGYVRLLQAMKVQFEGGGAVEEYTRLAQSGGTSSEVKMISLLNCYTVQAARVSRCSG
DKQGELVEQCNKVWLQVEKLLQETDLQFNPIWECTVTILWLFSHFEPFSWNPLPCSDK
QRAEYVSKLREFYSSNKFVAGEAVADNRFKLKKALLLQILVNYLGGRMLEHDLGEIYA
ISAKCFDMCRQQGGMRKVQYVIGIWHLMNCTVAMRGKDVALTNAKLEALVKQITSVKQ
"
gene <465303..>466025
/gene="SPT15"
/locus_tag="YER148W"
/gene_synonym="BTF1; TBP1"
/db_xref="GeneID:856891"
mRNA <465303..>466025
/gene="SPT15"
/locus_tag="YER148W"
/gene_synonym="BTF1; TBP1"
/product="TATA-binding protein"
/transcript_id="NM_001179038.3"
/db_xref="GeneID:856891"
CDS 465303..466025
/gene="SPT15"
/locus_tag="YER148W"
/gene_synonym="BTF1; TBP1"
/experiment="EXISTENCE:direct assay:GO:0000126
transcription factor TFIIIB complex
[PMID:1423590|PMID:1458536]"
/experiment="EXISTENCE:direct assay:GO:0000979 RNA
polymerase II core promoter sequence-specific DNA binding
[PMID:2677740]"
/experiment="EXISTENCE:direct assay:GO:0001016 RNA
polymerase III transcription regulatory region
sequence-specific DNA binding [PMID:8662956]"
/experiment="EXISTENCE:direct assay:GO:0001179 RNA
polymerase I general transcription initiation factor
binding [PMID:8895657]"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:19098311]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9818719]"
/experiment="EXISTENCE:direct assay:GO:0005669
transcription factor TFIID complex
[PMID:15448131|PMID:10788514]"
/experiment="EXISTENCE:direct assay:GO:0008301 DNA
binding, bending [PMID:1736286]"
/experiment="EXISTENCE:direct assay:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:12501245]"
/experiment="EXISTENCE:direct assay:GO:0070898 RNA
polymerase III preinitiation complex assembly
[PMID:1480467]"
/experiment="EXISTENCE:mutant phenotype:GO:0001188 RNA
polymerase I preinitiation complex assembly
[PMID:8895657]"
/experiment="EXISTENCE:mutant phenotype:GO:0042790
nucleolar large rRNA transcription by RNA polymerase I
[PMID:1586947]"
/experiment="EXISTENCE:mutant phenotype:GO:0051123 RNA
polymerase II preinitiation complex assembly
[PMID:12840001]"
/experiment="EXISTENCE:physical interaction:GO:0001179 RNA
polymerase I general transcription initiation factor
binding [PMID:8895657]"
/experiment="EXISTENCE:physical interaction:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:12501245]"
/note="TATA-binding protein (TBP); general transcription
factor that interacts with other factors to form the
preinitiation complex at promoters; essential for
viability, highly conserved; yeast gene can complement
mutations in human homolog TBP"
/codon_start=1
/product="TATA-binding protein"
/protein_id="NP_011075.3"
/db_xref="GeneID:856891"
/db_xref="SGD:S000000950"
/translation="MADEERLKEFKEANKIVFDPNTRQVWENQNRDGTKPATTFQSEE
DIKRAAPESEKDTSATSGIVPTLQNIVATVTLGCRLDLKTVALHARNAEYNPKRFAAV
IMRIREPKTTALIFASGKMVVTGAKSEDDSKLASRKYARIIQKIGFAAKFTDFKIQNI
VGSCDVKFPIRLEGLAFSHGTFSSYEPELFPGLIYRMVKPKIVLLIFVSGKIVLTGAK
QREEIYQAFEAIYPVLSEFRKM"
gene complement(<466208..>467470)
/gene="PEA2"
/locus_tag="YER149C"
/gene_synonym="DFG9; PPF2"
/db_xref="GeneID:856892"
mRNA complement(<466208..>467470)
/gene="PEA2"
/locus_tag="YER149C"
/gene_synonym="DFG9; PPF2"
/product="Pea2p"
/transcript_id="NM_001179039.1"
/db_xref="GeneID:856892"
CDS complement(466208..467470)
/gene="PEA2"
/locus_tag="YER149C"
/gene_synonym="DFG9; PPF2"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:8909546]"
/experiment="EXISTENCE:direct assay:GO:0000133 polarisome
[PMID:9632790]"
/experiment="EXISTENCE:direct assay:GO:0005933 cellular
bud [PMID:8909546]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:8909546]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:8909546]"
/experiment="EXISTENCE:direct assay:GO:0030427 site of
polarized growth [PMID:33656555]"
/experiment="EXISTENCE:direct assay:GO:0031489 myosin V
binding [PMID:33656555]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:8909546|PMID:19053807]"
/experiment="EXISTENCE:genetic interaction:GO:0007118
budding cell apical bud growth [PMID:10866679]"
/experiment="EXISTENCE:mutant phenotype:GO:0000753 cell
morphogenesis involved in conjugation with cellular fusion
[PMID:8909546|PMID:8013906]"
/experiment="EXISTENCE:mutant phenotype:GO:0007118 budding
cell apical bud growth [PMID:10866679]"
/experiment="EXISTENCE:mutant phenotype:GO:0007121 bipolar
cellular bud site selection [PMID:8909546]"
/experiment="EXISTENCE:mutant phenotype:GO:0007124
pseudohyphal growth [PMID:9055077]"
/experiment="EXISTENCE:mutant phenotype:GO:0030010
establishment of cell polarity
[PMID:33656555|PMID:8013906|PMID:8909546]"
/experiment="EXISTENCE:mutant phenotype:GO:0030447
filamentous growth [PMID:15645503]"
/experiment="EXISTENCE:mutant phenotype:GO:0032880
regulation of protein localization
[PMID:10085294|PMID:11740491|PMID:8909546]"
/experiment="EXISTENCE:mutant phenotype:GO:0032956
regulation of actin cytoskeleton organization
[PMID:17237521|PMID:19633059]"
/experiment="EXISTENCE:mutant phenotype:GO:0071468
cellular response to acidic pH [PMID:19633059]"
/experiment="EXISTENCE:mutant phenotype:GO:0071474
cellular hyperosmotic response [PMID:17237521]"
/note="Coiled-coil 12S polarisome subunit; required for
polarity establishment, apical bud growth, shmoo
formation, filamentous differentiation; involved in Bni1p
localization at sites of polarized growth, controlling
polarized assembly of actin cables; role in apical growth
affects diploid-specific bipolar bud site selection;
retains Slt2p at bud tip to regulate ER inheritance; role
in Ca2+ influx, cell fusion; S288C allele encoding Leu409
rather than Met linked with non-invasion"
/codon_start=1
/product="Pea2p"
/protein_id="NP_011076.1"
/db_xref="GeneID:856892"
/db_xref="SGD:S000000951"
/translation="MHKFDLELSRRANPLLFSAERYEEYPLKYDELKQYLLSQNPSHP
HHNSRPYTSIDYFDYLLYRSKNDESEIDLDKKLVSEFALYYVQKEHLNSDDLNPTLNE
LLKLQPKSADWYEMMLRILESINTTGIDQLTKENNNSFPNSKRARSSTNMGGTDKFNK
GAYHTDKADDDKNEILQELTSFLMSNSIQKGIDIKPIPLDDPVKFLKNGINSILDTCV
SLEKNTSSPPISPNAAAIQEEDSSKKLEELETAFSDLQLAHNFLTKQFENDRAEYVQD
IEKLTRTNRELQDKLLNNHSNLSKTEKKLHELEQENKELEKANNKLNSSRHNFGMSSP
ASSPVTWDPSSPSSVGSPTSGSGSRSLSIMTSEFKKVLTSTQRKYEKELSDEREHRFK
LERELALLKNAEANTSLALNRDDPPDML"
gene <468370..>468816
/gene="SPI1"
/locus_tag="YER150W"
/db_xref="GeneID:856893"
mRNA <468370..>468816
/gene="SPI1"
/locus_tag="YER150W"
/product="Spi1p"
/transcript_id="NM_001179040.1"
/db_xref="GeneID:856893"
CDS 468370..468816
/gene="SPI1"
/locus_tag="YER150W"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:9613572]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0010447
response to acidic pH [PMID:16980434]"
/note="GPI-anchored cell wall protein involved in weak
acid resistance; basal expression requires Msn2p/Msn4p;
expression is induced under conditions of stress and
during the diauxic shift; SPI1 has a paralog, SED1, that
arose from the whole genome duplication"
/codon_start=1
/product="Spi1p"
/protein_id="NP_011077.1"
/db_xref="GeneID:856893"
/db_xref="SGD:S000000952"
/translation="MLSNAKLLLSLAMASTALGLVSNSSSSVIVVPSSDATIAGNDTA
TPAPEPSSAAPIFYNSTATATQYEVVSEFTTYCPEPTTFVTNGATFTVTAPTTLTITN
CPCTIEKPTSETSVSSTHDVETNSNAANARAIPGALGLAGAVMMLL"
gene 469457..469530
/locus_tag="YNCE0026W"
/db_xref="GeneID:856894"
tRNA 469457..469530
/locus_tag="YNCE0026W"
/product="tRNA-Val"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Valine tRNA (tRNA-Val), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:856894"
/db_xref="SGD:S000006754"
gene complement(<469686..>472424)
/gene="UBP3"
/locus_tag="YER151C"
/gene_synonym="BLM3"
/db_xref="GeneID:856895"
mRNA complement(<469686..>472424)
/gene="UBP3"
/locus_tag="YER151C"
/gene_synonym="BLM3"
/product="mRNA-binding ubiquitin-specific protease UBP3"
/transcript_id="NM_001179041.3"
/db_xref="GeneID:856895"
CDS complement(469686..472424)
/gene="UBP3"
/locus_tag="YER151C"
/gene_synonym="BLM3"
/EC_number="3.4.19.12"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:20844764|PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0004843
cysteine-type deubiquitinase activity [PMID:1429680]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0016579 protein
deubiquitination [PMID:18498751]"
/experiment="EXISTENCE:genetic interaction:GO:0045053
protein retention in Golgi apparatus [PMID:32673164]"
/experiment="EXISTENCE:mutant phenotype:GO:0004843
cysteine-type deubiquitinase activity [PMID:23476013]"
/experiment="EXISTENCE:mutant phenotype:GO:0016579 protein
deubiquitination
[PMID:23645675|PMID:23476013|PMID:18498751]"
/experiment="EXISTENCE:mutant phenotype:GO:0034517
ribophagy [PMID:18391941]"
/experiment="EXISTENCE:mutant phenotype:GO:0045053 protein
retention in Golgi apparatus [PMID:32673164]"
/experiment="EXISTENCE:mutant phenotype:GO:0047484
regulation of response to osmotic stress [PMID:21743437]"
/experiment="EXISTENCE:mutant phenotype:GO:0060628
regulation of ER to Golgi vesicle-mediated transport
[PMID:12778054]"
/experiment="EXISTENCE:physical interaction:GO:0047484
regulation of response to osmotic stress [PMID:21743437]"
/experiment="EXISTENCE:physical interaction:GO:1990861
Ubp3-Bre5 deubiquitination complex [PMID:12778054]"
/note="Ub-specific protease; Ubp3p-Bre5p complex subunit
that mediates retention of Golgi membrane proteins;
deubiquitinates Sec23p and Sec27p with Bre5p, regulating
Golgi-ER retrograde transport; cleaves K48-linked di-Ub
and K48/K63 mixed-linkage polyUb chains from Rps20p on
translating ribosomes to enhance RQC; phosphorylated by
Hog1p to regulate the osmotic stress response; inhibits
Ras/PKA signaling through Ira2p; role in ribophagy;
protein abundance increases in response to DNA replication
stress"
/codon_start=1
/product="mRNA-binding ubiquitin-specific protease UBP3"
/protein_id="NP_011078.3"
/db_xref="GeneID:856895"
/db_xref="SGD:S000000953"
/translation="MNMQDANKEESYSMYPKTSSPPPPTPTNMQIPIYQAPLQMYGYT
QAPYLYPTQIPAYSFNMVNQNQPIYHQSGSPHHLPPQNNINGGSTTNNNNINKKKWHS
NGITNNNGSSGNQGANSSGSGMSYNKSHTYHHNYSNNHIPMMASPNSGSNAGMKKQTN
SSNGNGSSATSPSYSSYNSSSQYDLYKFDVTKLKNLKENSSNLIQLPLFINTTEAEFA
AASVQRYELNMKALNLNSESLENSSVEKSSAHHHTKSHSIPKHNEEVKTETHGEEEDA
HDKKPHASKDAHELKKKTEVKKEDAKQDRNEKVIQEPQATVLPVVDKKEPEESVEENT
SKTSSPSPSPPAAKSWSAIASDAIKSRQASNKTVSGSMVTKTPISGTTAGVSSTNMAA
ATIGKSSSPLLSKQPQKKDKKYVPPSTKGIEPLGSIALRMCFDPDFISYVLRNKDVEN
KIPVHSIIPRGIINRANICFMSSVLQVLLYCKPFIDVINVLSTRNTNSRVGTSSCKLL
DACLTMYKQFDKETYEKKFLENADDAEKTTESDAKKSSKSKSFQHCATADAVKPDEFY
KTLSTIPKFKDLQWGHQEDAEEFLTHLLDQLHEELISAIDGLTDNEIQNMLQSINDEQ
LKVFFIRNLSRYGKAEFIKNASPRLKELIEKYGVINDDSTEENGWHEVSGSSKRGKKT
KTAAKRTVEIVPSPISKLFGGQFRSVLDIPNNKESQSITLDPFQTIQLDISDAGVNDL
ETAFKKFSEYELLPFKSSSGNDVEAKKQTFIDKLPQVLLIQFKRFSFINNVNKDNAMT
NYNAYNGRIEKIRKKIKYGHELIIPEESMSSITLKNNTSGIDDRRYKLTGVIYHHGVS
SDGGHYTADVYHSEHNKWYRIDDVNITELEDDDVLKGGEEASDSRTAYILMYQKRN"
gene complement(<472657..>473988)
/locus_tag="YER152C"
/db_xref="GeneID:856896"
mRNA complement(<472657..>473988)
/locus_tag="YER152C"
/product="2-aminoadipate transaminase"
/transcript_id="NM_001179042.3"
/db_xref="GeneID:856896"
CDS complement(472657..473988)
/locus_tag="YER152C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0047536
2-aminoadipate transaminase activity [PMID:19342587]"
/note="Protein with 2-aminoadipate transaminase activity;
shares amino acid similarity with the aminotransferases
Aro8p and Aro9p; YER152C is not an essential gene"
/codon_start=1
/product="2-aminoadipate transaminase"
/protein_id="NP_011079.3"
/db_xref="GeneID:856896"
/db_xref="SGD:S000000954"
/translation="MKYKEINFFKGHPSSRLLPREAVIQATAAILGPETREYDNDPYN
RHPLTYGSDEGALWVREQICTFLNDQLFKFENGARSRTRADYLNLNSGASYGMLNILL
QTTLPHNGYTRQAFIITPTYFLINNCFTDAGFKGKMTAINEQGHDSIDFESLISALEQ
HEAEPQPHSTTEMIQGPKLTKKVYRYVMYCIPTFANPSGNTYSLETRRRLIDIARKYD
MLIITDDVYDILDYTTPSDELPSPPLRMVHIDRSTAPSGEDSFGNTVSNATFSKLIAP
GLRFGYHESINANLARQLSKGGANVSGGTPSQLNSMIVGEMLRSGAAQRCIAHLRSVY
SERATVLTSALKKYMPLGTEIMPLKGGYFTWITLPPAYNAMEISTILAKKFNVILADG
SNFEVIGDEKNWGQSCFRLSISFLEVDDIDRGIELFGAVCKSHAITNNITM"
gene complement(<474041..>474805)
/gene="PET122"
/locus_tag="YER153C"
/db_xref="GeneID:856897"
mRNA complement(<474041..>474805)
/gene="PET122"
/locus_tag="YER153C"
/product="Pet122p"
/transcript_id="NM_001179043.1"
/db_xref="GeneID:856897"
CDS complement(474041..474805)
/gene="PET122"
/locus_tag="YER153C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:12529447|PMID:8389363]"
/experiment="EXISTENCE:mutant phenotype:GO:0003743
translation initiation factor activity [PMID:2833749]"
/experiment="EXISTENCE:mutant phenotype:GO:0033617
mitochondrial respiratory chain complex IV assembly
[PMID:2833749]"
/experiment="EXISTENCE:mutant phenotype:GO:0070131
positive regulation of mitochondrial translation
[PMID:2833749]"
/note="Mitochondrial translational activator specific for
the COX3 mRNA; acts together with Pet54p and Pet494p;
located in the mitochondrial inner membrane"
/codon_start=1
/product="Pet122p"
/protein_id="NP_011080.1"
/db_xref="GeneID:856897"
/db_xref="SGD:S000000955"
/translation="MLTITKRLVTTDVRSRILLSSLNGKMSDALALLRQQQQTSVDVE
LLHTMLARAAALAHADTIAYMWYQHVMPRRLPVEGRLLCEMAGVALYQDRLFLPAQFL
QHYQAMNRDRRTSPEDELIEYELRRIKVEAFARGTMHSTALREKWKVFLQEMDTLPGQ
PPLRLRDFPQMTKAMGIALMQQDEQAAALALFGRQPLVIKNEWSLPLLLAGVLWHVPG
PAQARRVLAEFRQSYRGLPLLDAELVIKRRGFEINT"
gene <475020..>476228
/gene="OXA1"
/locus_tag="YER154W"
/db_xref="GeneID:856898"
mRNA <475020..>476228
/gene="OXA1"
/locus_tag="YER154W"
/product="membrane insertase OXA1"
/transcript_id="NM_001179044.1"
/db_xref="GeneID:856898"
CDS 475020..476228
/gene="OXA1"
/locus_tag="YER154W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0030061
mitochondrial crista [PMID:22513091]"
/experiment="EXISTENCE:direct assay:GO:0097002
mitochondrial inner boundary membrane [PMID:22513091]"
/experiment="EXISTENCE:direct assay:GO:0097177
mitochondrial ribosome binding [PMID:14657018]"
/experiment="EXISTENCE:mutant phenotype:GO:0032977
membrane insertase activity [PMID:9428747|PMID:9285818]"
/experiment="EXISTENCE:mutant phenotype:GO:0032979 protein
insertion into mitochondrial inner membrane from matrix
[PMID:9285818|PMID:9482871|PMID:9428747|PMID:17452441]"
/experiment="EXISTENCE:mutant phenotype:GO:0033615
mitochondrial proton-transporting ATP synthase complex
assembly [PMID:17344477]"
/experiment="EXISTENCE:mutant phenotype:GO:0045039 protein
insertion into mitochondrial inner membrane
[PMID:22846909]"
/note="Mitochondrial inner membrane insertase; mediates
the insertion of both mitochondrial- and nuclear-encoded
proteins from the matrix into the inner membrane; also has
a role in insertion of carrier proteins into the inner
membrane; acts as a voltage-gated ion channel, activated
by substrate peptides; interacts with mitochondrial
ribosomes; conserved from bacteria to animals"
/codon_start=1
/product="membrane insertase OXA1"
/protein_id="NP_011081.1"
/db_xref="GeneID:856898"
/db_xref="SGD:S000000956"
/translation="MFKLTSRLVTSRFAASSRLATARTIVLPRPHPSWISFQAKRFNS
TGPNANDVSEIQTQLPSIDELTSSAPSLSASTSDLIANTTQTVGELSSHIGYLNSIGL
AQTWYWPSDIIQHVLEAVHVYSGLPWWGTIAATTILIRCLMFPLYVKSSDTVARNSHI
KPELDALNNKLMSTTDLQQGQLVAMQRKKLLSSHGIKNRWLAAPMLQIPIALGFFNAL
RHMANYPVDGFANQGVAWFTDLTQADPYLGLQVITAAVFISFTRLGGETGAQQFSSPM
KRLFTILPIISIPATMNLSSAVVLYFAFNGAFSVLQTMILRNKWVRSKLKITEVAKPR
TPIAGASPTENMGIFQSLKHNIQKARDQAERRQLMQDNEKKLQESFKEKRQNSKIKIV
HKSNFINNKK"
gene complement(<476345..>482848)
/gene="BEM2"
/locus_tag="YER155C"
/gene_synonym="IPL2; SUP9; TSL1"
/db_xref="GeneID:856899"
mRNA complement(<476345..>482848)
/gene="BEM2"
/locus_tag="YER155C"
/gene_synonym="IPL2; SUP9; TSL1"
/product="GTPase-activating protein BEM2"
/transcript_id="NM_001179045.3"
/db_xref="GeneID:856899"
CDS complement(476345..482848)
/gene="BEM2"
/locus_tag="YER155C"
/gene_synonym="IPL2; SUP9; TSL1"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:17914457]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005096 GTPase
activator activity [PMID:7962098|PMID:12145202]"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:17914457]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:17914457]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:17914457]"
/experiment="EXISTENCE:direct assay:GO:0005938 cell cortex
[PMID:17914457]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:17914457]"
/experiment="EXISTENCE:genetic interaction:GO:0035024
negative regulation of Rho protein signal transduction
[PMID:17914457]"
/experiment="EXISTENCE:mutant phenotype:GO:0005096 GTPase
activator activity [PMID:12145202]"
/experiment="EXISTENCE:mutant phenotype:GO:0030036 actin
cytoskeleton organization [PMID:7579704]"
/experiment="EXISTENCE:mutant phenotype:GO:0035024
negative regulation of Rho protein signal transduction
[PMID:17914457]"
/experiment="EXISTENCE:mutant phenotype:GO:0044879 mitotic
morphogenesis checkpoint signaling [PMID:12145202]"
/note="Rho GTPase activating protein (RhoGAP); contains
RhoGAP activity specific for both Rho1p and Cdc42p, and
potentially Rho4p; acts with the RhoGAP Bem3p to
negatively regulate Cdc42p-dependent signal transduction;
involved in the control of cytoskeleton organization and
cellular morphogenesis; required for bud emergence and for
the morphogenesis checkpoint in response to actin
depolymerization; localizes to sites of polarized growth
and the plasma membrane"
/codon_start=1
/product="GTPase-activating protein BEM2"
/protein_id="NP_011082.3"
/db_xref="GeneID:856899"
/db_xref="SGD:S000000957"
/translation="MKGLLWSKNRKSSTASASSSSTSTSHKTTTASTASSSSPSSSSQ
TIRNSTSGASPYMHSHHHHGQGHSHHRGEDNNRDKRKSSVFPPSKQYTSTSSSQVNLG
MYHSDTNTRSSRSIASTLKDDSPSVCSEDEISNSSSQKSNAQDETPIAYKKSAHSKDS
LLPSRSSSLSPPQSRCSTGTTLEKSLNTSGISNSSGTNNNNSNNNNDNEQKQRNVIHL
NSENYDTTVFKTGWVNKSHGQTVATNYNSSMTAPSSSSSSSSQNLRNDAYSRNRESRF
YGNDGSSLKNDDSSSTTATNSGNDVASARSSMAIDPQMLVPDYRLYRAQLKGCVLNLY
KSGLNSNIKFFDPTLPASNSSIANENHQQKKQQTNNQAQAEALHQKQSFGQMGEPITL
DLKYLSEVYPHPDLRQDSDGKIISGTIESLCHTVLFYPGPKQSDVPNEKSLSKTHRAV
INLLLMFPLLDHFIKFLKVFNQFGLSFTKNKSRLTNNSTQFYNISPAVDDSMTQRLAL
TAKTILDVFPGFLLDEPMLKTIISLLDTISLHNDEISNNLKIKIANKHNELMKLTAFT
RSLPMATSSTHELEIILDPSHFLSLDITTLADEVHHINLKFDKVWAPKFDYSLLYDSK
FINRRIVSLNPLVFNNDQNIHFLGRLLISHLFPTNPEFSKKVTPKVRAELLDKWVQIG
CRFEHLGDMVSWLAVATIICSIPVLRSSSWKYVPDQSLKTIFKDWVPTIIQLERRQRT
SKSTSSVFILAPPNLDDDFTRANVISYFGDLLIHADDLPSDTKFKYLEKKINRTKNAF
HKWQQRLQAIDSTRHKTNSTENVRDNDSPNNVVYQLWKFHLSQPPLNIEGIMKLSVQH
EPPIIDQKAYSTIGSQRSALVTGSYLPILFNELFPNYSLFPKNTLVGAASDAKLPPPR
SSARLSKSLSISEPIPIASNSHTMGSLTDDAMSSKNDNNKVTGVGKIDGPVIKEMSSK
QSNKQRLLKSVRDVFNIDMDVFHISDELVFKSVYDNDGKSRPASMVIETPKRFSQHSS
MLINNPATPNQKMRDSLDTTGRLSKTLENMDFFNNIGQVSDSLKESIIRVVLKSSSLE
KIFDLLVLTSNIFSKLVDTKDLENYYYHQRQRGHSTRGLSDDNIGLLDYAFVKLTMDN
DIFTETFFNTYKSFTTTTTVLENMAKRYVGAKSCSVSISKILDRSDDSKMKINEDTNL
VSSSLYDQNFPVWDMKVTDDENINLIYMAKIQIGAAEAILHLVKNHYSDFTDDLCNNS
TLLDIIKIMEQEVSTEWPTRIANSKLQKSLPENFVIETENLLTTLTDLFHGIKSAYQK
QLYRPIGVNRTQKRITDILNSFNTFSFTDLNNIIDDPSFSDDMIRSFQKLHSTNYEDI
LEWIYQLDNFISKKFNLVSKKDWIVLFQELELLSKESLVSFFNYPLHFKSSKLINPGY
LQLHEFEISNLFTWISTLILKDDNGTESLFFEKLPQSIKLLIKLHTSLTTFFVMEISN
VNKSSSERLTTCKVILQILNYIRWKNGSLDLFDSEEDESPHAICPHIPAFIETAIAHA
IISPESRNYELSWIKASEKLSDPTKGTQNLRSISNVLEKIDDIHIKRFIEIDDVFSKN
CKNLCPCPGWFISRLLEISQFVPNMSITNSKLINFDKRRFVNNIISNVLDLIPNEREF
PLDIEMSDENPSKRTTFGRILFNNFEDVNKVYRKKTKKVSESEAISERFQEQGVFNEI
LVNEIEKIKREARKLEVLLDQEKILKNSAALHQAVPKKNRKSVIISGTHSDNDHSYNI
NKNTGQTPSLGSVMESNNSARNRRDSRASFSTNRSSVVSNSSHNGVSKKIGGFFRRPF
SIGGFNTSSSNYSLNSILSQEVSSNKSILPSILPEVDSMQLHDLKPSYSLKTFEIKSI
MEIINHRNIPAYYYAFKIVMQNGHEYLIQTASSSDLTEWIKMIKASKRFSFHSKKYKG
KTHNKIFGVPLEDVCERENTLIPTIVVKLLEEIELRGLDEVGLYRIPGSIGSINALKN
AFDEEGATDNSFTLEDDRWFEVNAIAGCFKMYLRELPDSLFSHAMVNDFTDLAIKYKA
HAMVNEEYKRMMNELLQKLPTCYYQTLKRIVFHLNKVHQHVVNNKMDASNLAIVFSMS
FINQEDLANSMGSRLGAVQTILQDFIKNPNDYFKQ"
gene complement(<483325..>484341)
/gene="MYG1"
/locus_tag="YER156C"
/db_xref="GeneID:856900"
mRNA complement(<483325..>484341)
/gene="MYG1"
/locus_tag="YER156C"
/product="Myg1p"
/transcript_id="NM_001179046.3"
/db_xref="GeneID:856900"
CDS complement(483325..484341)
/gene="MYG1"
/locus_tag="YER156C"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:31323700]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095|PMID:31323700]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:1901857
positive regulation of cellular respiration
[PMID:31081026]"
/note="Protein involved in mitochondrial function;
interacts with Hsp82p and copurifies with Ipl1p;
expression is copper responsive and downregulated in
strains deleted for MAC1, a copper-responsive
transcription factor; role in preventing L-A mycovirus
pathogenesis; human homolog MYG1 is linked to
developmental processes and dermatological diseases,
complements yeast null mutant"
/codon_start=1
/product="Myg1p"
/protein_id="NP_011083.3"
/db_xref="GeneID:856900"
/db_xref="SGD:S000000958"
/translation="MNSVKRVKLNSKMSKQICTHSGSFHADESLAVYMLRLLPEFKDA
KLVRSRNPKDWEASDILVDVGAQYDGVKFFDHHQRGFFETFNEKYKTKLSSAGLIFKH
YGRDIIKTILNNKVSSSDLDLLYDKVYKQFVEALDANDNGISKYTIPKDSNLEPNFRD
NAISIPGIISGMNPNWNEDTSDESFDRCFARASEFIGGVFVTLVRGYGQSWLPAKALV
AQAIDERMDVDKSGKIIVLPQFCPWKEHLYELEREKNIEKQIEFVLFTDSSGAWRVST
VPINSTSFQFRRGLPEPLRGLRDEELSTKSGVPGCIFIHAAGFIGGAKSKEAVYELAK
MSLA"
gene <484788..>487193
/gene="COG3"
/locus_tag="YER157W"
/gene_synonym="GRD20; SEC34"
/db_xref="GeneID:856901"
mRNA <484788..>487193
/gene="COG3"
/locus_tag="YER157W"
/gene_synonym="GRD20; SEC34"
/product="Golgi transport complex subunit COG3"
/transcript_id="NM_001179047.1"
/db_xref="GeneID:856901"
CDS 484788..487193
/gene="COG3"
/locus_tag="YER157W"
/gene_synonym="GRD20; SEC34"
/experiment="EXISTENCE:genetic interaction:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:10512869]"
/experiment="EXISTENCE:mutant phenotype:GO:0000301
retrograde transport, vesicle recycling within Golgi
[PMID:11703943]"
/experiment="EXISTENCE:mutant phenotype:GO:0000425
pexophagy [PMID:20065092]"
/experiment="EXISTENCE:mutant phenotype:GO:0006891
intra-Golgi vesicle-mediated transport [PMID:11703943]"
/experiment="EXISTENCE:mutant phenotype:GO:0016236
macroautophagy [PMID:20065092]"
/experiment="EXISTENCE:mutant phenotype:GO:0017119 Golgi
transport complex [PMID:11703943]"
/experiment="EXISTENCE:mutant phenotype:GO:0032258
cytoplasm to vacuole targeting by the Cvt pathway
[PMID:20065092]"
/experiment="EXISTENCE:mutant phenotype:GO:0140312 cargo
adaptor activity [PMID:15229219]"
/note="Essential component of the conserved oligomeric
Golgi complex; a cytosolic tethering complex (Cog1p
through Cog8p) that functions in protein trafficking to
mediate fusion of transport vesicles to Golgi
compartments"
/codon_start=1
/product="Golgi transport complex subunit COG3"
/protein_id="NP_011084.1"
/db_xref="GeneID:856901"
/db_xref="SGD:S000000959"
/translation="MARSRKNSLVRDIASHPTIPESQTIVGLLDDSYLFDKLKKLSLA
VENSDSLQRTDVSEGCSEVNGSEATTSADVKKTNKYLYYTTYLDQLNIKIDEYKVVLD
QTRQVNDQLDSSIKKFRKISQDTGAFIEETKTIYEKQSKLSNLTESIPKALHYFEVLD
PIMRRLNHATSPAIVKKSSFTTMLATIDESLRFLDENSDLKDAAAYRIKFKQCLIRAC
ELISHFLTNLLKQTNQEILDKTKNKNSLTGLPSTTRDAFLYSKFYTIADTFKIQVSEI
VKRSNEKAYNKYHDELNSILYECFNHYFQTRLRLLTPVIWSHIDEIVVKDKDQGLVKF
IQDGKVYFQQLCADEYKLFVEFFPEKECRFKINQWFLQLCEPLYDSIRVRVLKETDIC
TLCDSVTLFAPYYEFEEGSEEYVKQFTDIQYDKLFEPIVQKVQARLILRVQIYVQQNI
LSYRPTRDVFMISNRRRKSKTSLQGGNEDATTSDDNPDPLLESYLSSFKNRSILPISP
NDADDKSIDSEESTDKISQLQTYYPPLLKTLALLSKIYEMINSVVFDDLAHHVVHDCI
VSLRNAYDMVIKSSAGKSDFNNLDISLAYLKNLLMLRDSIQNFNIQYTVNETYLDFSG
VEGFFKSLKENGRNVLKKTKSSSILTLARELVPKVVNNMVDARTELISELRNVIKDFT
ESTSLELIDDTLDINSDEDLLSKNVKLRENIKARLPRIYEQILNYIDDQEIVTNLLDA
VQELITQSYSKYYETITELAENGKFAKDQVADVMYLDVFTDFFAKEVADLLRNGDIDT
ITK"
gene complement(487331..487402)
/locus_tag="YNCE0027C"
/db_xref="GeneID:856902"
tRNA complement(487331..487402)
/locus_tag="YNCE0027C"
/product="tRNA-Glu"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE
analysis; thiolation of uridine at wobble position (34)
requires Ncs6p; target of K. lactis zymocin"
/db_xref="GeneID:856902"
/db_xref="SGD:S000006552"
repeat_region 487834..488165
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006920"
gene complement(<488857..>490578)
/locus_tag="YER158C"
/db_xref="GeneID:856903"
mRNA complement(<488857..>490578)
/locus_tag="YER158C"
/product="uncharacterized protein"
/transcript_id="NM_001179048.1"
/db_xref="GeneID:856903"
CDS complement(488857..490578)
/locus_tag="YER158C"
/note="hypothetical protein; potentially phosphorylated by
Cdc28p; YER158C has a paralog, AFR1, that arose from the
whole genome duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011085.1"
/db_xref="GeneID:856903"
/db_xref="SGD:S000000960"
/translation="MLQQGSSSRRSLHGNDFHTLTSPSRRDSLSIPRAVDARSASTID
LFYIPDATVSRRHSTLVANRSDNNGNGAPMRQYNKPNFASSSTSSLPSTRNRPSRYDN
MNMNMNMNMNMNMNMNMNMNNHTTSDHNAHPQYRCRPNPSRRHSLMTIPEKYSGSRYS
LRSSPPTYSNPRVRKELTPFQLQRKQMKSAFQFPNGENFTPRNQIARLPPSSTFPDSP
SSSSLPLTQTGGPSSADNDSIATGTNNRSPQQTKAADANQKSESESPKAIRSNSKKIS
RFFRKIWSSKSSNSADSVEENSKTKQKRKNPERVVPEPITSLDQPVEIIKQSFSTVNN
HETAVPSIKDSGIVQELTALGDNNRIPVLPPPRSPNRPTLSDKRTTKLYYCSQDSSNE
DIAPEEKSTVFLKRLQDEWSTVYLNKLPLTASVPSSLSTTTDAANSSFINSSISSPAP
SSSSSSSLVSRGPMQSISSSPTPAPSSGSSKSKNAVKSLRFADEIYVNDTWSAADYCR
CDNTFLNNFFKGKSQDITNPSTFVGNNLSSTKNISNIEIKMEVNEFKRKEMRVHQDSA
KYTHYYL"
gene complement(<491530..>491958)
/gene="BUR6"
/locus_tag="YER159C"
/gene_synonym="NCB1"
/db_xref="GeneID:856904"
mRNA complement(<491530..>491958)
/gene="BUR6"
/locus_tag="YER159C"
/gene_synonym="NCB1"
/product="negative cofactor 2 transcription regulator
complex subunit BUR6"
/transcript_id="NM_001179049.3"
/db_xref="GeneID:856904"
CDS complement(491530..491958)
/gene="BUR6"
/locus_tag="YER159C"
/gene_synonym="NCB1"
/experiment="EXISTENCE:direct assay:GO:0000122 negative
regulation of transcription by RNA polymerase II
[PMID:8948634|PMID:9096360|PMID:9023340]"
/experiment="EXISTENCE:direct assay:GO:0001046 core
promoter sequence-specific DNA binding [PMID:18703679]"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:18703679]"
/experiment="EXISTENCE:direct assay:GO:0003713
transcription coactivator activity [PMID:12237409]"
/experiment="EXISTENCE:direct assay:GO:0003714
transcription corepressor activity [PMID:9023340]"
/experiment="EXISTENCE:direct assay:GO:0017054 negative
cofactor 2 complex [PMID:8948634]"
/experiment="EXISTENCE:direct assay:GO:0045944 positive
regulation of transcription by RNA polymerase II
[PMID:12237409]"
/experiment="EXISTENCE:genetic interaction:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:10713169]"
/experiment="EXISTENCE:genetic interaction:GO:0003714
transcription corepressor activity [PMID:10713169]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:10713169]"
/experiment="EXISTENCE:mutant phenotype:GO:0003714
transcription corepressor activity [PMID:10713169]"
/experiment="EXISTENCE:mutant phenotype:GO:0034605
cellular response to heat [PMID:18048413]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:18048413]"
/experiment="EXISTENCE:mutant phenotype:GO:0051123 RNA
polymerase II preinitiation complex assembly
[PMID:18048413]"
/experiment="EXISTENCE:physical interaction:GO:0017025
TBP-class protein binding [PMID:9096360]"
/experiment="EXISTENCE:physical interaction:GO:0017054
negative cofactor 2 complex [PMID:9023340]"
/note="Subunit of a heterodimeric NC2 transcription
regulator complex; complex binds to TBP and can repress
transcription by preventing preinitiation complex assembly
or stimulate activated transcription; homologous to human
NC2alpha; complex also includes Ncb2p; bur6 ncb2 double
mutation is functionally complemented by coexpression of
human DRAP1 and DR1, although the single bur6 mutation is
not complemented by its ortholog DRAP1"
/codon_start=1
/product="negative cofactor 2 transcription regulator
complex subunit BUR6"
/protein_id="NP_011086.3"
/db_xref="GeneID:856904"
/db_xref="SGD:S000000961"
/translation="MADQVPVTTQLPPIKPEHEVPLDAGGSPVGNMGTNSNNNNELGD
VFDRIKTHFPPAKVKKIMQTDEDIGKVSQATPVIAGRSLEFFIALLVKKSGEMARGQG
TKRITAEILKKTILNDEKFDFLREGLCVEEGQTQPEEESA"
gene complement(492352..492424)
/locus_tag="YNCE0028C"
/db_xref="GeneID:856905"
tRNA complement(492352..492424)
/locus_tag="YNCE0028C"
/product="tRNA-Arg"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE
analysis; one of 6 nuclear tRNA genes containing the
tDNA-anticodon ACG (converted to ICG in the mature tRNA),
decodes CGU, CGC, and probably CGA codons into arginine,
one of 19 nuclear tRNAs for arginine"
/db_xref="GeneID:856905"
/db_xref="SGD:S000006701"
mobile_element complement(492695..498421)
/note="YERCTy1-2; Ty1 element, LTR retrotransposon of the
Copia (Pseudoviridae) group; contains co-transcribed genes
TYA Gag and TYB Pol, encoding proteins involved in
structure and function of virus-like particles, flanked by
two direct repeats"
/mobile_element_type="retrotransposon:YERCTy1-2"
/db_xref="SGD:S000006911"
repeat_region complement(492695..492833)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006900"
gene complement(<492856..>498124)
/locus_tag="YER160C"
/db_xref="GeneID:856906"
mRNA complement(<492856..>498124)
/locus_tag="YER160C"
/product="gag-pol fusion protein"
/transcript_id="NM_001179050.4"
/db_xref="GeneID:856906"
CDS complement(join(492856..496821,496823..498124))
/locus_tag="YER160C"
/EC_number="2.7.7.7"
/EC_number="2.7.7.49"
/EC_number="3.1.26.4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9448009]"
/ribosomal_slippage
/note="Retrotransposon TYA Gag and TYB Pol genes;
transcribed/translated as one unit; polyprotein is
processed to make a nucleocapsid-like protein (Gag),
reverse transcriptase (RT), protease (PR), and integrase
(IN); similar to retroviral genes"
/codon_start=1
/product="gag-pol fusion protein"
/protein_id="NP_011087.3"
/db_xref="GeneID:856906"
/db_xref="SGD:S000000962"
/translation="MESQQLSNYPHISHGSACASVTSKEVHTNQDPLDVSASKIQEYD
KASTKANSQQTTTPASSAVPENPHHASPQPASVPPPQNGPYPQQCMMTQNQANPSGWS
FYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVRQITDDELT
FLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQ
YNGSTPADAFETKVTNIIDRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNM
TVAELFLDIHAIYEEQQGSRNSKPNYRRNPSDEKNDSRSYTNTTKPKVIARNPQKTNN
SKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLGQKLTESTVNHTNHS
DDELPGHLLLDSGASRTLIRSAHHIHSASSNPDINVVDAQKRNIPINAIGDLQFHFQD
NTKTSIKVLHTPNIAYDLLSLNELAAVDITACFTKNVLERSDGTVLAPIVKYGDFYWV
SKKYLLPSNISVPTINNVHTSESTRKYPYPFIHRMLAHANAQTIRYSLKNNTITYFNE
SDVDWSSAIDYQCPDCLIGKSTKHRHIKGSRLKYQNSYEPFQYLHTDIFGPVHNLPKS
APSYFISFTDETTKFRWVYPLHDRREDSILDVFTTILAFIKNQFQASVLVIQMDRGSE
YTNRTLHKFLEKNGITPCYTTTADSRAHGVAERLNRTLLDDCRTQLQCSGLPNHLWFS
AIEFSTIVRNSLASPKSKKSARQHAGLAGLDISTLLPFGQPVIVNDHNPNSKIHPRGI
PGYALHPSRNSYGYIIYLPSLKKTVDTTNYVILQGKESRLDQFNYDALTFDEDLNRLT
ASYHSFIASNEIQQSNDLNIESDHDFQSDIELHPEQLRNVLSKAVSPTDSTPPSTHTE
DSKRVSKTNIRAPREVDPNISESNILPSKKRSSTPQISDIESTGSGGMHRLDVPLLAP
MSQSNTHESSHASKSKDFRHSDSYSDNETNHTNVPISSTGGTNNKTVPQTSEQETEKR
IIHRSPSIDTSSSESNSLHHVVPIKTSDTCPKENTEESIIADLPLPDLPPEPPTELSD
SFKELPPINSHQTNSSLGGIGDSNAYTTINSKKRSLEDNETEIKVSRDTWNTKNMRSL
EPPRSKKRIHLIAAVKAVKSIKPIRTTLRYDEAITYNKDIKEKEKYIQAYHKEVNQLL
KMKTWDTDRYYDRKEIDPKRVINSMFIFNRKRDGTHKARFVARGDIQHPDTYDPGMQS
NTVHHYALMTSLSLALDNNYYITQLDISSAYLYADIKEELYIRPPPHLGMNDKLIRLK
KSLYGLKQSGANWYETIKSYLIKQCGMEEVRGWSCVFKNSQVTICLFVDDMILFSKDL
NANKKIITTLKKQYDTKIINLGESDNEIQYDILGLEIKYQRGKYMKLGMENSLTEKIP
KLNVPLNPKGRKLSAPGQPGLYIDQQELELEEDDYKMKVHEMQKLIGLASYVGYKFRF
DLLYYINTLAQHILFPSKQVLDMTYELIQFIWNTRDKQLIWHKSKPVKPTNKLVVISD
ASYGNQPYYKSQIGNIYLLNGKVIGGKSTKASLTCTSTTEAEIHAISESVPLLNNLSH
LVQELNKKPITKGLLTDSKSTISIIISNNEEKFRNRFFGTKAMRLRDEVSGNHLHVCY
IETKKNIADVMTKPLPIKTFKLLTNKWIH"
gene complement(<496802..>498124)
/locus_tag="YER159C-A"
/db_xref="GeneID:856907"
mRNA complement(<496802..>498124)
/locus_tag="YER159C-A"
/product="gag protein"
/transcript_id="NM_001184429.1"
/db_xref="GeneID:856907"
CDS complement(496802..498124)
/locus_tag="YER159C-A"
/note="Retrotransposon TYA Gag gene co-transcribed with
TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is
a nucleocapsid protein that is the structural constituent
of virus-like particles (VLPs); similar to retroviral Gag"
/codon_start=1
/product="gag protein"
/protein_id="NP_058156.1"
/db_xref="GeneID:856907"
/db_xref="SGD:S000007403"
/translation="MESQQLSNYPHISHGSACASVTSKEVHTNQDPLDVSASKIQEYD
KASTKANSQQTTTPASSAVPENPHHASPQPASVPPPQNGPYPQQCMMTQNQANPSGWS
FYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVRQITDDELT
FLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQ
YNGSTPADAFETKVTNIIDRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNM
TVAELFLDIHAIYEEQQGSRNSKPNYRRNPSDEKNDSRSYTNTTKPKVIARNPQKTNN
SKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLRPETY"
repeat_region complement(498084..498421)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006901"
rep_origin 498884..499182
/note="ARS520; Autonomously Replicating Sequence"
/db_xref="SGD:S000077392"
gene complement(<499347..>500348)
/gene="SPT2"
/locus_tag="YER161C"
/gene_synonym="EXA1; SIN1"
/db_xref="GeneID:856908"
mRNA complement(<499347..>500348)
/gene="SPT2"
/locus_tag="YER161C"
/gene_synonym="EXA1; SIN1"
/product="Spt2p"
/transcript_id="NM_001179051.1"
/db_xref="GeneID:856908"
CDS complement(499347..500348)
/gene="SPT2"
/locus_tag="YER161C"
/gene_synonym="EXA1; SIN1"
/experiment="EXISTENCE:direct assay:GO:0000217 DNA
secondary structure binding [PMID:15563464]"
/experiment="EXISTENCE:direct assay:GO:0003677 DNA binding
[PMID:2072912]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476|PMID:2072912]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:genetic interaction:GO:0140673
transcription elongation-coupled chromatin remodeling
[PMID:16449659]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:17526727]"
/experiment="EXISTENCE:mutant phenotype:GO:0006325
chromatin organization [PMID:16449659|PMID:19057669]"
/note="Protein involved in negative regulation of
transcription; required for RNA polyadenylation; exhibits
regulated interactions with both histones and SWI-SNF
components; relocalizes to the cytosol in response to
hypoxia; similar to mammalian HMG1 proteins"
/codon_start=1
/product="Spt2p"
/protein_id="NP_011088.1"
/db_xref="GeneID:856908"
/db_xref="SGD:S000000963"
/translation="MSFLSKLSQIRKSTTASKAQVQDPLPKKNDEEYSLLPKNYIRDE
DPAVKRLKELRRQELLKNGALAKKSGVKRKRGTSSGSEKKKIERNDDDEGGLGIRFKR
SIGASHAPLKPVVRKKPEPIKKMSFEELMKQAENNEKQPPKVKSSEPVTKERPHFNKP
GFKSSKRPQKKASPGATLRGVSSGGNSIKSSDSPKPVKLNLPTNGFAQPNRRLKEKLE
SRKQKSRYQDDYDEEDNDMDDFIEDDEDEGYHSKSKHSNGPGYDRDEIWAMFNRGKKR
SEYDYDELEDDDMEANEMEILEEEEMARKMARLEDKREEAWLKKHEEEKRRRKKGIR"
gene complement(<500630..>502894)
/gene="RAD4"
/locus_tag="YER162C"
/db_xref="GeneID:856909"
mRNA complement(<500630..>502894)
/gene="RAD4"
/locus_tag="YER162C"
/product="Rad4p"
/transcript_id="NM_001179052.4"
/db_xref="GeneID:856909"
CDS complement(500630..502894)
/gene="RAD4"
/locus_tag="YER162C"
/experiment="EXISTENCE:direct assay:GO:0000111
nucleotide-excision repair factor 2 complex
[PMID:7768886]"
/experiment="EXISTENCE:direct assay:GO:0003684 damaged DNA
binding [PMID:9813069|PMID:9837874]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:19889839]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:19889839]"
/experiment="EXISTENCE:direct assay:GO:0006265 DNA
topological change [PMID:27035942]"
/experiment="EXISTENCE:direct assay:GO:1990165
single-strand break-containing DNA binding
[PMID:27035942]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:26150418]"
/experiment="EXISTENCE:mutant phenotype:GO:0006289
nucleotide-excision repair [PMID:18936173]"
/experiment="EXISTENCE:mutant phenotype:GO:0043161
proteasome-mediated ubiquitin-dependent protein catabolic
process [PMID:19889839]"
/note="Damaged-DNA binding subunit of Nuclear Excision
Repair Factor 2; recognizes and binds damaged DNA with
Rad23p during nucleotide excision repair (NER); also
involved with Rad23p in turnover of ubiquitylated
proteins; Rad4p-Rad23p heterodimer binds to promoters of
DNA damage response genes to repress their transcription
in the absence of DNA damage; RAD4 and RAD51 pathways
confer resistance to benzo[a]pyrene dihydrodiol"
/codon_start=1
/product="Rad4p"
/protein_id="NP_011089.4"
/db_xref="GeneID:856909"
/db_xref="SGD:S000000964"
/translation="MNEDLPKEYFELIRKALNEKEAEKAPLSRRRRVRRKNQPLPDAK
KKFKTGLNELPRESVVTVNLDSSDDGVVTVPTDDSVEEIQSSEEDYDSEEFEDVTDGN
EVAGVEDISVEIKPSSKRNSDARRTSRNVCSNEERKRRKYFHMLYLVCLMVHGFIRNE
WINSKRLSRKLSNLVPEKVFELLHPQKDEELPLRSTRKLLDGLKKCMELWQKHWKITK
KYDNVGLYMRTWKEIEMSANNKRKFKTLKRSDFLRAVSKGHGDPDISVQGFVAMLRAC
NVNARLIMSCQPPDFTNMKIDTSLNGNNAYKDMVKYPIFWCEVWDKFSKKWITVDPVN
LKTIEQVRLHSKLAPKGVACCERNMLRYVIAYDRKYGCRDVTRRYAQWMNSKVRKRRI
TKDDFGEKWFRKVITALHHRKRTKIDDYEDQYFFQRDESEGIPDSVQDLKNHPYYVLE
QDIKQTQIVKPGCKECGYLKVHGKVGKVLKVYAKRDIADLKSARQWYMNGRILKTGSR
CKKVIKRTVGRPKGEAEEEDERLYSFEDTELYIPPLASASGEITKNTFGNIEVFAPTM
IPGNCCLVENPVAIKAARFLGVEFAPAVTSFKFERGSTVKPVLSGIVVAKWLREAIET
AIDGIEFIQEDDNRKEHLLGALESWNTLLLKLRIRSKLNSTYGKIAEEEPNVTKEQNI
ADNHDNTETFMGGGFLPGIANHEARPYSEPSEPEDSLDYVSVDKAEESATDDDVGEDY
SDFMKELEMSEESD"
gene complement(<503084..>503782)
/gene="GCG1"
/locus_tag="YER163C"
/db_xref="GeneID:856910"
mRNA complement(<503084..>503782)
/gene="GCG1"
/locus_tag="YER163C"
/product="gamma-glutamylcyclotransferase"
/transcript_id="NM_001179053.3"
/db_xref="GeneID:856910"
CDS complement(503084..503782)
/gene="GCG1"
/locus_tag="YER163C"
/EC_number="4.3.2.7"
/experiment="EXISTENCE:direct assay:GO:0003839
gamma-glutamylcyclotransferase activity [PMID:23070364]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006751 glutathione
catabolic process [PMID:23070364]"
/experiment="EXISTENCE:mutant phenotype:GO:0006751
glutathione catabolic process [PMID:23070364]"
/note="Gamma-glutamyl cyclotransferase; cleaves the
gamma-glutamyl bond of glutathione to yield 5-oxoproline
and a Cys-Gly dipeptide; similar to mammalian
pro-apoptotic protein ChaC1; expression of mouse ChaC1 in
yeast increases apoptosis; green fluorescent protein
(GFP)-fusion protein localizes to the cytoplasm and
nucleus; periodically expressed during the metabolic
cycle"
/codon_start=1
/product="gamma-glutamylcyclotransferase"
/protein_id="NP_011090.3"
/db_xref="GeneID:856910"
/db_xref="SGD:S000000965"
/translation="MTNDNSGIWVLGYGSLIYKPPSHYTHRIPAIIHGFARRFWQSST
DHRGTPANPGRVATLIPYEDIIRQTAFLKNVNLYSESAPIQDPDDLVTIGVVYYIPPE
HAQEVREYLNVREQNGYTLHEVEVHLETNREHEAELGEALEQLPRHNKSGKRVLLTSV
YIGTIDNEAFVGPETVDETAKVIAVSHGPSGSNYEYLAKLEQALAQMPIMKERGRITD
HYLTALLETVNKYR"
gene <505392..>509798
/gene="CHD1"
/locus_tag="YER164W"
/db_xref="GeneID:856911"
mRNA <505392..>509798
/gene="CHD1"
/locus_tag="YER164W"
/product="chromatin-remodeling ATPase CHD1"
/transcript_id="NM_001179054.1"
/db_xref="GeneID:856911"
CDS 505392..509798
/gene="CHD1"
/locus_tag="YER164W"
/experiment="EXISTENCE:direct assay:GO:0000124 SAGA
complex [PMID:15647753]"
/experiment="EXISTENCE:direct assay:GO:0000182 rDNA
binding [PMID:17259992]"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:12504018|PMID:12682017]"
/experiment="EXISTENCE:direct assay:GO:0000976
transcription cis-regulatory region binding
[PMID:23468649]"
/experiment="EXISTENCE:direct assay:GO:0003677 DNA binding
[PMID:21623345]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0006338 chromatin
remodeling [PMID:33174727|PMID:10811623]"
/experiment="EXISTENCE:direct assay:GO:0008094
ATP-dependent activity, acting on DNA [PMID:10811623]"
/experiment="EXISTENCE:direct assay:GO:0030874 nucleolar
chromatin [PMID:17259992]"
/experiment="EXISTENCE:direct assay:GO:0031490 chromatin
DNA binding [PMID:12682017]"
/experiment="EXISTENCE:direct assay:GO:0035861 site of
double-strand break [PMID:34520455]"
/experiment="EXISTENCE:direct assay:GO:0046695 SLIK
(SAGA-like) complex [PMID:15647753]"
/experiment="EXISTENCE:direct assay:GO:0140002 histone
H3K4me3 reader activity [PMID:15647753]"
/experiment="EXISTENCE:direct assay:GO:0140658
ATP-dependent chromatin remodeler activity
[PMID:33174727|PMID:10811623]"
/experiment="EXISTENCE:genetic interaction:GO:0001178
regulation of transcriptional start site selection at RNA
polymerase II promoter [PMID:19948887]"
/experiment="EXISTENCE:genetic interaction:GO:0006363
termination of RNA polymerase I transcription
[PMID:17259992]"
/experiment="EXISTENCE:genetic interaction:GO:0006368
transcription elongation by RNA polymerase II
[PMID:12682017]"
/experiment="EXISTENCE:genetic interaction:GO:0006369
termination of RNA polymerase II transcription
[PMID:12504018]"
/experiment="EXISTENCE:genetic interaction:GO:0140750
nucleosome array spacer activity
[PMID:26861626|PMID:21940898]"
/experiment="EXISTENCE:genetic interaction:GO:2000104
negative regulation of DNA-templated DNA replication
[PMID:18245327]"
/experiment="EXISTENCE:mutant phenotype:GO:0000724
double-strand break repair via homologous recombination
[PMID:34520455]"
/experiment="EXISTENCE:mutant phenotype:GO:0000729 DNA
double-strand break processing [PMID:34520455]"
/experiment="EXISTENCE:mutant phenotype:GO:0006369
termination of RNA polymerase II transcription
[PMID:12504018]"
/experiment="EXISTENCE:mutant phenotype:GO:0007062 sister
chromatid cohesion [PMID:31222142]"
/experiment="EXISTENCE:mutant phenotype:GO:0034728
nucleosome organization [PMID:10811623]"
/experiment="EXISTENCE:mutant phenotype:GO:0140750
nucleosome array spacer activity [PMID:26861626]"
/experiment="EXISTENCE:mutant phenotype:GO:1902275
regulation of chromatin organization [PMID:22922743]"
/experiment="EXISTENCE:physical interaction:GO:0006368
transcription elongation by RNA polymerase II
[PMID:12682017]"
/note="Chromatin remodeler that regulates various aspects
of transcription; acts in conjunction with Isw1b to
regulate chromatin structure, maintain chromatin integrity
during transcription elongation by RNAP II by preventing
trans-histone exchange over coding regions; component of
both SAGA and SLIK complexes; ATPase motor stimulates DNA
unwrapping from nucleosomes; contains two chromodomains, a
helicase domain and a DNA-binding domain; human ortholog
CHD1 is a prostate cancer tumor suppressor"
/codon_start=1
/product="chromatin-remodeling ATPase CHD1"
/protein_id="NP_011091.1"
/db_xref="GeneID:856911"
/db_xref="SGD:S000000966"
/translation="MAAKDISTEVLQNPELYGLRRSHRAAAHQQNYFNDSDDEDDEDN
IKQSRRKRMTTIEDDEDEFEDEEGEEDSGEDEDEEDFEEDDDYYGSPIKQNRSKPKSR
TKSKSKSKPKSQSEKQSTVKIPTRFSNRQNKTVNYNIDYSDDDLLESEDDYGSEEALS
EENVHEASANPQPEDFHGIDIVINHRLKTSLEEGKVLEKTVPDLNNCKENYEFLIKWT
DESHLHNTWETYESIGQVRGLKRLDNYCKQFIIEDQQVRLDPYVTAEDIEIMDMERER
RLDEFEEFHVPERIIDSQRASLEDGTSQLQYLVKWRRLNYDEATWENATDIVKLAPEQ
VKHFQNRENSKILPQYSSNYTSQRPRFEKLSVQPPFIKGGELRDFQLTGINWMAFLWS
KGDNGILADEMGLGKTVQTVAFISWLIFARRQNGPHIIVVPLSTMPAWLDTFEKWAPD
LNCICYMGNQKSRDTIREYEFYTNPRAKGKKTMKFNVLLTTYEYILKDRAELGSIKWQ
FMAVDEAHRLKNAESSLYESLNSFKVANRMLITGTPLQNNIKELAALVNFLMPGRFTI
DQEIDFENQDEEQEEYIHDLHRRIQPFILRRLKKDVEKSLPSKTERILRVELSDVQTE
YYKNILTKNYSALTAGAKGGHFSLLNIMNELKKASNHPYLFDNAEERVLQKFGDGKMT
RENVLRGLIMSSGKMVLLDQLLTRLKKDGHRVLIFSQMVRMLDILGDYLSIKGINFQR
LDGTVPSAQRRISIDHFNSPDSNDFVFLLSTRAGGLGINLMTADTVVIFDSDWNPQAD
LQAMARAHRIGQKNHVMVYRLVSKDTVEEEVLERARKKMILEYAIISLGVTDGNKYTK
KNEPNAGELSAILKFGAGNMFTATDNQKKLEDLNLDDVLNHAEDHVTTPDLGESHLGG
EEFLKQFEVTDYKADIDWDDIIPEEELKKLQDEEQKRKDEEYVKEQLEMMNRRDNALK
KIKNSVNGDGTAANSDSDDDSTSRSSRRRARANDMDSIGESEVRALYKAILKFGNLKE
ILDELIADGTLPVKSFEKYGETYDEMMEAAKDCVHEEEKNRKEILEKLEKHATAYRAK
LKSGEIKAENQPKDNPLTRLSLKKREKKAVLFNFKGVKSLNAESLLSRVEDLKYLKNL
INSNYKDDPLKFSLGNNTPKPVQNWSSNWTKEEDEKLLIGVFKYGYGSWTQIRDDPFL
GITDKIFLNEVHNPVAKKSASSSDTTPTPSKKGKGITGSSKKVPGAIHLGRRVDYLLS
FLRGGLNTKSPSADIGSKKLPTGPSKKRQRKPANHSKSMTPEITSSEPANGPPSKRMK
ALPKGPAALINNTRLSPNSPTPPLKSKVSRDNGTRQSSNPSSGSAHEKEYDSMDEEDC
RHTMSAIRTSLKRLRRGGKSLDRKEWAKILKTELTTIGNHIESQKGSSRKASPEKYRK
HLWSYSANFWPADVKSTKLMAMYDKITESQKK"
gene <510373..>512106
/gene="PAB1"
/locus_tag="YER165W"
/db_xref="GeneID:856912"
mRNA <510373..>512106
/gene="PAB1"
/locus_tag="YER165W"
/product="polyadenylate-binding protein"
/transcript_id="NM_001179055.1"
/db_xref="GeneID:856912"
CDS 510373..512106
/gene="PAB1"
/locus_tag="YER165W"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:15630021|PMID:15769879|PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276|PMID:15769879|PMID:15630021]"
/experiment="EXISTENCE:direct assay:GO:0005840 ribosome
[PMID:8631901]"
/experiment="EXISTENCE:direct assay:GO:0006446 regulation
of translational initiation [PMID:10357826]"
/experiment="EXISTENCE:direct assay:GO:0008143 poly(A)
binding [PMID:9193001]"
/experiment="EXISTENCE:direct assay:GO:0008428
ribonuclease inhibitor activity [PMID:11889048]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule
[PMID:18981231|PMID:19470581|PMID:17908917|PMID:26777405|P
MID:24291094|PMID:40593583]"
/experiment="EXISTENCE:direct assay:GO:0060211 regulation
of nuclear-transcribed mRNA poly(A) tail shortening
[PMID:17766253]"
/experiment="EXISTENCE:direct assay:GO:1990841
promoter-specific chromatin binding [PMID:27184763]"
/experiment="EXISTENCE:mutant phenotype:GO:0006446
regulation of translational initiation [PMID:10357826]"
/experiment="EXISTENCE:mutant phenotype:GO:0008143 poly(A)
binding [PMID:9193001]"
/experiment="EXISTENCE:mutant phenotype:GO:0031124 mRNA
3'-end processing [PMID:9223284]"
/experiment="EXISTENCE:mutant phenotype:GO:0060211
regulation of nuclear-transcribed mRNA poly(A) tail
shortening [PMID:17766253]"
/note="Poly(A) binding protein; interacts with the
cleavage factor complex CF I, mediates interactions
between the 5' cap structure and the 3' mRNA poly(A) tail,
involved in control of poly(A) tail length, interacts with
translation factor eIF-4G; stimulates, but is not required
for the deadenylation activity of the Pan2p-Pan3p
poly(A)-ribonuclease complex; role in preventing L-A
mycovirus pathogenesis"
/codon_start=1
/product="polyadenylate-binding protein"
/protein_id="NP_011092.1"
/db_xref="GeneID:856912"
/db_xref="SGD:S000000967"
/translation="MADITDKTAEQLENLNIQDDQKQAATGSESQSVENSSASLYVGD
LEPSVSEAHLYDIFSPIGSVSSIRVCRDAITKTSLGYAYVNFNDHEAGRKAIEQLNYT
PIKGRLCRIMWSQRDPSLRKKGSGNIFIKNLHPDIDNKALYDTFSVFGDILSSKIATD
ENGKSKGFGFVHFEEEGAAKEAIDALNGMLLNGQEIYVAPHLSRKERDSQLEETKAHY
TNLYVKNINSETTDEQFQELFAKFGPIVSASLEKDADGKLKGFGFVNYEKHEDAVKAV
EALNDSELNGEKLYVGRAQKKNERMHVLKKQYEAYRLEKMAKYQGVNLFVKNLDDSVD
DEKLEEEFAPYGTITSAKVMRTENGKSKGFGFVCFSTPEEATKAITEKNQQIVAGKPL
YVAIAQRKDVRRSQLAQQIQARNQMRYQQATAAAAAAAAGMPGQFMPPMFYGVMPPRG
VPFNGPNPQQMNPMGGMPKNGMPPQFRNGPVYGVPPQGGFPRNANDNNQFYQQKQRQA
LGEQLYKKVSAKTSNEEAAGKITGMILDLPPQEVFPLLESDELFEQHYKEASAAYESF
KKEQEQQTEQA"
gene <512744..>517459
/gene="DNF1"
/locus_tag="YER166W"
/db_xref="GeneID:856913"
mRNA <512744..>517459
/gene="DNF1"
/locus_tag="YER166W"
/product="aminophospholipid-translocating P4-type ATPase
DNF1"
/transcript_id="NM_001179056.3"
/db_xref="GeneID:856913"
CDS 512744..517459
/gene="DNF1"
/locus_tag="YER166W"
/EC_number="7.6.2.1"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:33060204|PMID:12221123|PMID:12631737]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0070867 mating
projection tip membrane [PMID:25378585]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0006886
intracellular protein transport [PMID:12221123]"
/experiment="EXISTENCE:genetic interaction:GO:0006897
endocytosis [PMID:12631737]"
/experiment="EXISTENCE:genetic interaction:GO:0007163
establishment or maintenance of cell polarity
[PMID:15461661]"
/experiment="EXISTENCE:genetic interaction:GO:0045332
phospholipid translocation [PMID:12631737|PMID:17015438]"
/experiment="EXISTENCE:genetic interaction:GO:0090555
phosphatidylethanolamine flippase activity
[PMID:12631737|PMID:17015438]"
/experiment="EXISTENCE:genetic interaction:GO:0140345
phosphatidylcholine flippase activity [PMID:12631737]"
/experiment="EXISTENCE:mutant phenotype:GO:0006897
endocytosis [PMID:12631737]"
/experiment="EXISTENCE:mutant phenotype:GO:0045332
phospholipid translocation [PMID:17015438]"
/experiment="EXISTENCE:mutant phenotype:GO:0090555
phosphatidylethanolamine flippase activity
[PMID:17015438|PMID:27432949]"
/experiment="EXISTENCE:mutant phenotype:GO:0099040
ceramide translocation [PMID:33060204]"
/experiment="EXISTENCE:mutant phenotype:GO:0140345
phosphatidylcholine flippase activity [PMID:27432949]"
/experiment="EXISTENCE:mutant phenotype:GO:0140351
glycosylceramide flippase activity [PMID:33060204]"
/experiment="EXISTENCE:physical interaction:GO:1990531
phospholipid-translocating ATPase complex [PMID:15090616]"
/note="Aminophospholipid translocase (flippase); type 4
P-type ATPase; involved in phospholipid translocation,
contributing to endocytosis, protein transport, and
cellular polarization; localizes primarily to the plasma
membrane; localizes to the shmoo tip where it has a
redundant role in the cellular response to mating
pheromone; DNF1 has a paralog, DNF2, that arose from the
whole genome duplication"
/codon_start=1
/product="aminophospholipid-translocating P4-type ATPase
DNF1"
/protein_id="NP_011093.3"
/db_xref="GeneID:856913"
/db_xref="SGD:S000000968"
/translation="MSGTFHGDGHAPMSPFEDTFQFEDNSSNEDTHIAPTHFDDGATS
NKYSRPQVSFNDETPKNKREDAEEFTFNDDTEYDNHSFQPTPKLNNGSGTFDDVELDN
DSGEPHTNYDGMKRFRMGTKRNKKGNPIMGRSKTLKWARKNIPNPFEDFTKDDIDPGA
INRAQELRTVYYNMPLPKDMIDEEGNPIMQYPRNKIRTTKYTPLTFLPKNILFQFHNF
ANVYFLVLIILGAFQIFGVTNPGLSAVPLVVIVIITAIKDAIEDSRRTVLDLEVNNTK
THILEGVENENVSTDNISLWRRFKKANSRLLFKFIQYCKEHLTEEGKKKRMQRKRHEL
RVQKTVGTSGPRSSLDSIDSYRVSADYGRPSLDYDNLEQGAGEANIVDRSLPPRTDCK
FAKNYWKGVKVGDIVRIHNNDEIPADIILLSTSDTDGACYVETKNLDGETNLKVRQSL
KCTNTIRTSKDIARTKFWIESEGPHSNLYTYQGNMKWRNLADGEIRNEPITINNVLLR
GCTLRNTKWAMGVVMFTGGDTKIMLNSGITPTKKSRISRELNFSVVINFVLLFILCFV
SGIANGVYYDKKGRSRFSYEFGTIAGSAATNGFVSFWVAVILYQSLVPISLYISVEII
KTAQAAFIYGDVLLYNAKLDYPCTPKSWNISDDLGQVEYIFSDKTGTLTQNVMEFKKC
TINGVSYGRAYTEALAGLRKRQGIDVETEGRREKAEIAKDRDTMIDELRALSGNSQFY
PEEVTFVSKEFVRDLKGASGEVQQRCCEHFMLALALCHSVLVEANPDNPKKLDLKAQS
PDEAALVATARDVGFSFVGKTKKGLIIEMQGIQKEFEILNILEFNSSRKRMSCIVKIP
GLNPGDEPRALLICKGADSIIYSRLSRQSGSNSEAILEKTALHLEQYATEGLRTLCIA
QRELSWSEYEKWNEKYDIAAASLANREDELEVVADSIERELILLGGTAIEDRLQDGVP
DCIELLAEAGIKLWVLTGDKVETAINIGFSCNLLNNEMELLVIKTTGDDVKEFGSEPS
EIVDALLSKYLKEYFNLTGSEEEIFEAKKDHEFPKGNYAIVIDGDALKLALYGEDIRR
KFLLLCKNCRAVLCCRVSPSQKAAVVKLVKDSLDVMTLAIGDGSNDVAMIQSADVGIG
IAGEEGRQAVMCSDYAIGQFRYLARLVLVHGRWSYKRLAEMIPEFFYKNMIFALALFW
YGIYNDFDGSYLYEYTYMMFYNLAFTSLPVIFLGILDQDVNDTISLVVPQLYRVGILR
KEWNQRKFLWYMLDGLYQSIICFFFPYLVYHKNMIVTSNGLGLDHRYFVGVYVTTIAV
ISCNTYVLLHQYRWDWFSGLFIALSCLVVFAWTGIWSSAIASREFFKAAARIYGAPSF
WAVFFVAVLFCLLPRFTYDSFQKFFYPTDVEIVREMWQHGHFDHYPPGYDPTDPNRPK
VTKAGQHGEKIIEGIALSDNLGGSNYSRDSVVTEEIPMTFMHGEDGSPSGYQKQETWM
TSPKETQDLLQSPQFQQAQTFGRGPSTNVRSSLDRTREQMIATNQLDNRYSVERARTS
LDLPGVTNAASLIGTQQNN"
gene <518216..>520771
/gene="BCK2"
/locus_tag="YER167W"
/gene_synonym="CTR7"
/db_xref="GeneID:856914"
mRNA <518216..>520771
/gene="BCK2"
/locus_tag="YER167W"
/gene_synonym="CTR7"
/product="Bck2p"
/transcript_id="NM_001179057.3"
/db_xref="GeneID:856914"
CDS 518216..520771
/gene="BCK2"
/locus_tag="YER167W"
/gene_synonym="CTR7"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0000082 G1/S
transition of mitotic cell cycle [PMID:12518319]"
/experiment="EXISTENCE:mutant phenotype:GO:0010628
positive regulation of gene expression [PMID:19158491]"
/experiment="EXISTENCE:mutant phenotype:GO:0051726
regulation of cell cycle [PMID:7891677]"
/note="Serine/threonine-rich protein involved in PKC1
signaling pathway; protein kinase C (PKC1) signaling
pathway controls cell integrity; overproduction suppresses
pkc1 mutations"
/codon_start=1
/product="Bck2p"
/protein_id="NP_011094.3"
/db_xref="GeneID:856914"
/db_xref="SGD:S000000969"
/translation="MPKNSHHHRSSSVNSTKSRSTESTNKWKIPHYYRRSASGSTQAS
PDRNSSTGSCSTPVLPTMNVMSSPKKVLLEDPRDNHTKAKKSSRKKSGEMVFVNYTVQ
DTANENDTDLQTQPVSVPAPKAKLKKKSSKRRMLKIFGSSKNEHIEDIVEEQPMVLQM
DSESKPLSGTPISESGIDASSLTTKRSYNSFLKHNRLNGKTPFSGNLSFPSLNMMGNT
TDLPIDNNDFCSEKEVVPKSTHDPSLAKPPSRFTESETNSTPNLSSIPLMNTKNTRLK
YNKVAPQSSDRQKSQESGLYHSTESFNFKDQNYSNNKSSLSLNSDLSTPHFAKHSPDS
PRTSRSFNCGDSQSKVKLPEENDASIAFSKMFTRKRANTGGSTCSLASPTIAQTIQQS
NIKVNKLPTQRTTSVGSLSSMSNRYSPIRVASPGRARSATRGSSLYRLSRDLNSLPSV
TDLPEMDSTTPVNEIFLDGQPQHKSGSVKGGHRKKQESISDAQRIQHSNSYITTPSSS
LVTPPYYMTGYTLPSSASASSTPNVLETHNMNFVPSTSTVTSYRPSSNFSSFDKEYSN
ENDASGEFSAFNTPMENIPALKGIPRSTLEENEEEDVLVQDIPNTAHFQRRDIMGMDT
HRKDDSLDFNSLMPHGSTTSSSIVDSVMTNSISTTTSNATGNYFQDQDKYTLVNTGLG
LSDANLDHFIRSQWKHASRSESNNNTGNRVSYSGSTPNNVDTTKTNLQVYTEFDFENP
ESFFHEQSKLLGEMGHSNNNSNSAINMNEPKSADTYIGNISPDTSATVSLGDLMGSNV
SNNSERNFYDGHTFVPQYQANSSVENSNNQNAAPIANNDIDNNLQSFYFDNSN"
rep_origin 520772..521030
/note="ARS521; Autonomously Replicating Sequence"
/db_xref="SGD:S000077393"
gene complement(<521029..>522669)
/gene="CCA1"
/locus_tag="YER168C"
/gene_synonym="TNT1"
/db_xref="GeneID:856915"
mRNA complement(<521029..>522669)
/gene="CCA1"
/locus_tag="YER168C"
/gene_synonym="TNT1"
/product="tRNA adenylyltransferase"
/transcript_id="NM_001179058.1"
/db_xref="GeneID:856915"
CDS complement(521029..522669)
/gene="CCA1"
/locus_tag="YER168C"
/gene_synonym="TNT1"
/EC_number="2.7.7.72"
/experiment="EXISTENCE:direct assay:GO:0001680 tRNA
3'-terminal CCA addition [PMID:1634528]"
/experiment="EXISTENCE:direct assay:GO:0004810 CCA tRNA
nucleotidyltransferase activity [PMID:1634528]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:1634528]"
/experiment="EXISTENCE:direct assay:GO:0052927 CC tRNA
cytidylyltransferase activity [PMID:23872483]"
/experiment="EXISTENCE:direct assay:GO:0052929
ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity
[PMID:23872483]"
/note="ATP (CTP):tRNA-specific tRNA
nucleotidyltransferase; different forms targeted to the
nucleus, cytosol, and mitochondrion are generated via the
use of multiple transcriptional and translational start
sites; human homolog TRNT1 complements yeast null mutant"
/codon_start=1
/product="tRNA adenylyltransferase"
/protein_id="NP_011095.1"
/db_xref="GeneID:856915"
/db_xref="SGD:S000000970"
/translation="MLRSTISLLMNSAAQKTMTNSNFVLNAPKITLTKVEQNICNLLN
DYTDLYNQKYHNKPEPLTLRITGGWVRDKLLGQGSHDLDIAINVMSGEQFATGLNEYL
QQHYAKYGAKPHNIHKIDKNPEKSKHLETATTKLFGVEVDFVNLRSEKYTELSRIPKV
CFGTPEEDALRRDATLNALFYNIHKGEVEDFTKRGLQDLKDGVLRTPLPAKQTFLDDP
LRVLRLIRFASRFNFTIDPEVMAEMGDPQINVAFNSKISRERVGVEMEKILVGPTPLL
ALQLIQRAHLENVIFFWHNDSSVVKFNEENCQDMDKINHVYNDNILNSHLKSFIELYP
MFLEKLPILREKIGRSPGFQQNFILSAILSPMANLQIIGNPKKKINNLVSVTESIVKE
GLKLSKNDAAVIAKTVDSICSYEEILAKFADRSQLKKSEIGIFLRNFNGEWETAHFAS
LSDAFLKIPKLETKKIELLFQNYNEFYSYIFDNNLNNCHELKPIVDGKQMAKLLQMKP
GPWLGKINNEAIRWQFDNPTGTDQELITHLKAILPKYL"
gene <523369..>525759
/gene="RPH1"
/locus_tag="YER169W"
/gene_synonym="KDM4"
/db_xref="GeneID:856916"
mRNA <523369..>525759
/gene="RPH1"
/locus_tag="YER169W"
/gene_synonym="KDM4"
/product="Rph1p"
/transcript_id="NM_001179059.1"
/db_xref="GeneID:856916"
CDS 523369..525759
/gene="RPH1"
/locus_tag="YER169W"
/gene_synonym="KDM4"
/experiment="EXISTENCE:curator inference:GO:0005634
nucleus [PMID:10523651]"
/experiment="EXISTENCE:direct assay:GO:0000122 negative
regulation of transcription by RNA polymerase II
[PMID:10523651]"
/experiment="EXISTENCE:direct assay:GO:0001227 DNA-binding
transcription repressor activity, RNA polymerase
II-specific [PMID:10523651]"
/experiment="EXISTENCE:direct assay:GO:0032454 histone
H3K9 demethylase activity [PMID:17371840]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding
[PMID:21296759|PMID:19111667]"
/experiment="EXISTENCE:direct assay:GO:0051864 histone
H3K36 demethylase activity
[PMID:17525156|PMID:17369256|PMID:17371840]"
/experiment="EXISTENCE:genetic interaction:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:25660547]"
/experiment="EXISTENCE:genetic interaction:GO:0010507
negative regulation of autophagy [PMID:25660547]"
/experiment="EXISTENCE:genetic interaction:GO:0032968
positive regulation of transcription elongation by RNA
polymerase II [PMID:17525156]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:25660547]"
/experiment="EXISTENCE:mutant phenotype:GO:0001227
DNA-binding transcription repressor activity, RNA
polymerase II-specific [PMID:10523651]"
/experiment="EXISTENCE:mutant phenotype:GO:0010507
negative regulation of autophagy [PMID:25660547]"
/experiment="EXISTENCE:mutant phenotype:GO:0043565
sequence-specific DNA binding [PMID:21296759]"
/experiment="EXISTENCE:mutant phenotype:GO:0051864 histone
H3K36 demethylase activity
[PMID:17525156|PMID:17369256|PMID:17371840]"
/note="JmjC domain-containing histone demethylase; targets
tri- and dimethylated H3K36; associates with actively
transcribed regions and promotes elongation; repressor of
autophagy-related genes in nutrient-replete conditions;
damage-responsive repressor of PHR1; phosphorylated by the
Rad53p-dependent DNA damage checkpoint pathway and by a
Rim1p-mediated event during starvation; target of
stress-induced hormesis; RPH1 has a paralog, GIS1, that
arose from the whole genome duplication"
/codon_start=1
/product="Rph1p"
/protein_id="NP_011096.1"
/db_xref="GeneID:856916"
/db_xref="SGD:S000000971"
/translation="MTKLIAPSEIVGGVPVFKPTYEQFEDFYAYCKAINKYGMKSGVV
KVIPPKEWKDKLDLPYSAETLQKIKIKSPIQQHISGNKGLFMVQNVEKNKTYNIIQWK
DLSKDYVPPEDPKARRNSRKGSVSKSTKLKLKNFESSFNIDDFEQFRTEYTIDLSDFQ
NTERLKFLEEYYWKTLNFTTPMYGADTPGSIFPEGLNVWNVAKLPNILDHMETKVPGV
NDSYLYAGLWKASFSWHLEDQDLYSINYIHFGAPKQWYSIPQEDRFKFYKFMQEQFPE
EAKNCPEFLRHKMFLASPKLLQENGIRCNEIVHHEGEFMITYPYGYHAGFNYGYNLAE
SVNFALEEWLPIGKKAGKCHCISDSVEIDVKKLAKSWRDNNKESKGTPPLNQLPNPAM
PLLHRPTLKEMESSSLRSTSPDVGHFSNFKSKSSGVSSPLLSRMKDYSNIVEPTLEDP
TLKLKRISSFQEQPLNKLLKRETSQTAMLTDHEDNIVAMSLTSMANSAASSPRLPLSR
LNSSNELSNAQPLLDMTNNTLAFPRPNGPSGLNPLLYISNKNISGISHSAPHSPVNPN
ISLIKRVKSPNIVTLNISRESSRSPIALNYEARQQHSQQHSFSTPSTVSNLSTSVLGP
LSDTNDIKTPHPERPNHKTANRILKKESPVETSKSNLILSKVASTRQEDSFTSRNDDL
DKEQGSSPLNSKFAPEEIVLSGKNKIYICKECQRKFSSGHHLTRHKKSVHSGEKPHSC
PKCGKRFKRRDHVLQHLNKKIPCISNETTVDAPIMNPTVQPQDGKAAINQQSTPLN"
gene <525974..>526651
/gene="ADK2"
/locus_tag="YER170W"
/gene_synonym="AKY3; PAK3"
/db_xref="GeneID:856917"
mRNA <525974..>526651
/gene="ADK2"
/locus_tag="YER170W"
/gene_synonym="AKY3; PAK3"
/product="adenylate kinase ADK2"
/transcript_id="NM_001179060.3"
/db_xref="GeneID:856917"
CDS 525974..526651
/gene="ADK2"
/locus_tag="YER170W"
/gene_synonym="AKY3; PAK3"
/EC_number="2.7.4.10"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:15753074|PMID:8537371]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:8537371]"
/experiment="EXISTENCE:direct assay:GO:0009117 nucleotide
metabolic process [PMID:15753074]"
/experiment="EXISTENCE:direct assay:GO:0046899 nucleoside
triphosphate adenylate kinase activity
[PMID:15753074|PMID:8537371]"
/note="Mitochondrial adenylate kinase; catalyzes the
reversible synthesis of GTP and AMP from GDP and ADP; may
serve as a back-up for synthesizing GTP or ADP depending
on metabolic conditions; 3' sequence of ADK2 varies with
strain background"
/codon_start=1
/product="adenylate kinase ADK2"
/protein_id="NP_011097.3"
/db_xref="GeneID:856917"
/db_xref="SGD:S000000972"
/translation="MKADAKQITHLLKPLRLLLLGAPGSGKGTQTSRLLKQIPQLSSI
SSGDILRQEIKSESTLGREATTYIAQGKLLPDDLITRLITFRLSALGWLKPSAMWLLD
GFPRTTAQASALDELLKQHDASLNLVVELDVPESTILERIENRYVHVPSGRVYNLQYN
PPKVPGLDDITGEPLTKRLDDTAEVFKKRLEEYKKTNEPLKDYYKKSGIFGTVSGETS
DIIFRNY"
gene <527082..>529418
/gene="RAD3"
/locus_tag="YER171W"
/gene_synonym="REM1"
/db_xref="GeneID:856918"
mRNA <527082..>529418
/gene="RAD3"
/locus_tag="YER171W"
/gene_synonym="REM1"
/product="TFIIH/NER complex ATP-dependent 5'-3' DNA
helicase subunit RAD3"
/transcript_id="NM_001179061.3"
/db_xref="GeneID:856918"
CDS 527082..529418
/gene="RAD3"
/locus_tag="YER171W"
/gene_synonym="REM1"
/EC_number="5.6.2.3"
/experiment="EXISTENCE:direct assay:GO:0000112
nucleotide-excision repair factor 3 complex
[PMID:8855246]"
/experiment="EXISTENCE:direct assay:GO:0000439
transcription factor TFIIH core complex
[PMID:19818408|PMID:14500720]"
/experiment="EXISTENCE:direct assay:GO:0003684 damaged DNA
binding [PMID:8132553]"
/experiment="EXISTENCE:direct assay:GO:0005675
transcription factor TFIIH holo complex
[PMID:27381459|PMID:19818408]"
/experiment="EXISTENCE:direct assay:GO:0006366
transcription by RNA polymerase II [PMID:19818408]"
/experiment="EXISTENCE:direct assay:GO:0008094
ATP-dependent activity, acting on DNA [PMID:2957691]"
/experiment="EXISTENCE:direct assay:GO:0043139 5'-3' DNA
helicase activity [PMID:2827162]"
/experiment="EXISTENCE:mutant phenotype:GO:0000019
regulation of mitotic recombination
[PMID:10713167|PMID:9799251]"
/experiment="EXISTENCE:mutant phenotype:GO:0006289
nucleotide-excision repair [PMID:7026973]"
/experiment="EXISTENCE:mutant phenotype:GO:0045951
positive regulation of mitotic recombination
[PMID:24189665]"
/note="5' to 3' DNA helicase; involved in nucleotide
excision repair and transcription; subunit of RNA polII
initiation factor TFIIH and of Nucleotide Excision Repair
Factor 3 (NEF3); homolog of human XPD protein; mutant has
aneuploidy tolerance; protein abundance increases in
response to DNA replication stress"
/codon_start=1
/product="TFIIH/NER complex ATP-dependent 5'-3' DNA
helicase subunit RAD3"
/protein_id="NP_011098.3"
/db_xref="GeneID:856918"
/db_xref="SGD:S000000973"
/translation="MKFYIDDLPVLFPYPKIYPEQYNYMCDIKKTLDVGGNSILEMPS
GTGKTVSLLSLTIAYQMHYPEHRKIIYCSRTMSEIEKALVELENLMDYRTKELGYQED
FRGLGLTSRKNLCLHPEVSKERKGTVVDEKCRRMTNGQAKRKLEEDPEANVELCEYHE
NLYNIEVEDYLPKGVFSFEKLLKYCEEKTLCPYFIVRRMISLCNIIIYSYHYLLDPKI
AERVSNEVSKDSIVIFDEAHNIDNVCIESLSLDLTTDALRRATRGANALDERISEVRK
VDSQKLQDEYEKLVQGLHSADILTDQEEPFVETPVLPQDLLTEAIPGNIRRAEHFVSF
LKRLIEYLKTRMKVLHVISETPKSFLQHLKQLTFIERKPLRFCSERLSLLVRTLEVTE
VEDFTALKDIATFATLISTYEEGFLLIIEPYEIENAAVPNPIMRFTCLDASIAIKPVF
ERFSSVIITSGTISPLDMYPRMLNFKTVLQKSYAMTLAKKSFLPMIITKGSDQVAISS
RFEIRNDPSIVRNYGSMLVEFAKITPDGMVVFFPSYLYMESIVSMWQTMGILDEVWKH
KLILVETPDAQETSLALETYRKACSNGRGAILLSVARGKVSEGIDFDHQYGRTVLMIG
IPFQYTESRILKARLEFMRENYRIRENDFLSFDAMRHAAQCLGRVLRGKDDYGVMVLA
DRRFSRKRSQLPKWIAQGLSDADLNLSTDMAISNTKQFLRTMAQPTDPKDQEGVSVWS
YEDLIKHQNSRKDQGGFIENENKEGEQDEDEDEDIEMQ"
gene complement(<529530..>536021)
/gene="BRR2"
/locus_tag="YER172C"
/gene_synonym="PRP44; RSS1; SLT22; SNU246"
/db_xref="GeneID:856919"
mRNA complement(<529530..>536021)
/gene="BRR2"
/locus_tag="YER172C"
/gene_synonym="PRP44; RSS1; SLT22; SNU246"
/product="ATP-dependent RNA helicase BRR2"
/transcript_id="NM_001179062.1"
/db_xref="GeneID:856919"
CDS complement(529530..536021)
/gene="BRR2"
/locus_tag="YER172C"
/gene_synonym="PRP44; RSS1; SLT22; SNU246"
/EC_number="3.6.4.13"
/experiment="EXISTENCE:direct assay:GO:0003724 RNA
helicase activity [PMID:8649518]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005682 U5 snRNP
[PMID:11720284]"
/experiment="EXISTENCE:direct assay:GO:0046540 U4/U6 x U5
tri-snRNP complex
[PMID:8670905|PMID:10377396|PMID:10449419]"
/experiment="EXISTENCE:mutant phenotype:GO:0000388
spliceosome conformational change to release U4 (or
U4atac) and U1 (or U11) [PMID:9705931]"
/experiment="EXISTENCE:mutant phenotype:GO:0003724 RNA
helicase activity [PMID:8649518|PMID:9705931]"
/note="RNA-dependent ATPase RNA helicase (DEIH box);
required for disruption of U4/U6 base-pairing in native
snRNPs to activate the spliceosome for catalysis;
homologous to human U5-200kD"
/codon_start=1
/product="ATP-dependent RNA helicase BRR2"
/protein_id="NP_011099.1"
/db_xref="GeneID:856919"
/db_xref="SGD:S000000974"
/translation="MTEHETKDKAKKIREIYRYDEMSNKVLKVDKRFMNTSQNPQRDA
EISQPKSMSGRISAKDMGQGLCNNINKGLKENDVAVEKTGKSASLKKIQQHNTILNSS
SDFRLHYYPKDPSNVETYEQILQWVTEVLGNDIPHDLIIGTADIFIRQLKENEENEDG
NIEERKEKIQHELGINIDSLKFNELVKLMKNITDYETHPDNSNKQAVAILADDEKSDE
EEVTEMSNNANVLGGEINDNEDDDEEYDYNDVEVNSKKKNKRALPNIENDIIKLSDSK
TSNIESVPIYSIDEFFLQRKLRSELGYKDTSVIQDLSEKILNDIETLEHNPVALEQKL
VDLLKFENISLAEFILKNRSTIFWGIRLAKSTENEIPNLIEKMVAKGLNDLVEQYKFR
ETTHSKRELDSGDDQPQSSEAKRTKFSNPAIPPVIDLEKIKFDESSKLMTVTKVSLPE
GSFKRVKPQYDEIHIPAPSKPVIDYELKEITSLPDWCQEAFPSSETTSLNPIQSKVFH
AAFEGDSNMLICAPTGSGKTNIALLTVLKALSHHYNPKTKKLNLSAFKIVYIAPLKAL
VQEQVREFQRRLAFLGIKVAELTGDSRLSRKQIDETQVLVSTPEKWDITTRNSNNLAI
VELVRLLIIDEIHLLHDDRGPVLESIVARTFWASKYGQEYPRIIGLSATLPNYEDVGR
FLRVPKEGLFYFDSSFRPCPLSQQFCGIKERNSLKKLKAMNDACYEKVLESINEGNQI
IVFVHSRKETSRTATWLKNKFAEENITHKLTKNDAGSKQILKTEAANVLDPSLRKLIE
SGIGTHHAGLTRSDRSLSEDLFADGLLQVLVCTATLAWGVNLPAHTVIIKGTDVYSPE
KGSWEQLSPQDVLQMLGRAGRPRYDTFGEGIIITDQSNVQYYLSVLNQQLPIESQFVS
KLVDNLNAEVVAGNIKCRNDAVNWLAYTYLYVRMLASPMLYKVPDISSDGQLKKFRES
LVHSALCILKEQELVLYDAENDVIEATDLGNIASSFYINHASMDVYNRELDEHTTQID
LFRIFSMSEEFKYVSVRYEEKRELKQLLEKAPIPIREDIDDPLAKVNVLLQSYFSQLK
FEGFALNSDIVFIHQNAGRLLRAMFEICLKRGWGHPTRMLLNLCKSATTKMWPTNCPL
RQFKTCPVEVIKRLEASTVPWGDYLQLETPAEVGRAIRSEKYGKQVYDLLKRFPKMSV
TCNAQPITRSVMRFNIEIIADWIWDMNVHGSLEPFLLMLEDTDGDSILYYDVLFITPD
IVGHEFTLSFTYELKQHNQNNLPPNFFLTLISENWWHSEFEIPVSFNGFKLPKKFPPP
TPLLENISISTSELGNDDFSEVFEFKTFNKIQSQVFESLYNSNDSVFVGSGKGTGKTA
MAELALLNHWRQNKGRAVYINPSGEKIDFLLSDWNKRFSHLAGGKIINKLGNDPSLNL
KLLAKSHVLLATPVQFELLSRRWRQRKNIQSLELMIYDDAHEISQGVYGAVYETLISR
MIFIATQLEKKIRFVCLSNCLANARDFGEWAGMTKSNIYNFSPSERIEPLEINIQSFK
DVEHISFNFSMLQMAFEASAAAAGNRNSSSVFLPSRKDCMEVASAFMKFSKAIEWDML
NVEEEQIVPYIEKLTDGHLRAPLKHGVGILYKGMASNDERIVKRLYEYGAVSVLLISK
DCSAFACKTDEVIILGTNLYDGAEHKYMPYTINELLEMVGLASGNDSMAGKVLILTSH
NMKAYYKKFLIEPLPTESYLQYIIHDTLNNEIANSIIQSKQDCVDWFTYSYFYRRIHV
NPSYYGVRDTSPHGISVFLSNLVETCLNDLVESSFIEIDDTEAEVTAEVNGGDDEATE
IISTLSNGLIASHYGVSFFTIQSFVSSLSNTSTLKNMLYVLSTAVEFESVPLRKGDRA
LLVKLSKRLPLRFPEHTSSGSVSFKVFLLLQAYFSRLELPVDFQNDLKDILEKVVPLI
NVVVDILSANGYLNATTAMDLAQMLIQGVWDVDNPLRQIPHFNNKILEKCKEINVETV
YDIMALEDEERDEILTLTDSQLAQVAAFVNNYPNVELTYSLNNSDSLISGVKQKITIQ
LTRDVEPENLQVTSEKYPFDKLESWWLVLGEVSKKELYAIKKVTLNKETQQYELEFDT
PTSGKHNLTIWCVCDSYLDADKELSFEINVK"
gene <536300..>538279
/gene="RAD24"
/locus_tag="YER173W"
/db_xref="GeneID:856920"
mRNA <536300..>538279
/gene="RAD24"
/locus_tag="YER173W"
/product="Rad24p"
/transcript_id="NM_001179063.1"
/db_xref="GeneID:856920"
CDS 536300..538279
/gene="RAD24"
/locus_tag="YER173W"
/experiment="EXISTENCE:direct assay:GO:0003689 DNA clamp
loader activity [PMID:12604797]"
/experiment="EXISTENCE:direct assay:GO:0031389 Rad17
RFC-like complex [PMID:10660302|PMID:12604797]"
/experiment="EXISTENCE:mutant phenotype:GO:0000077 DNA
damage checkpoint signaling [PMID:9564050]"
/experiment="EXISTENCE:mutant phenotype:GO:0006289
nucleotide-excision repair [PMID:11267834]"
/experiment="EXISTENCE:mutant phenotype:GO:0007131
reciprocal meiotic recombination [PMID:10511543]"
/experiment="EXISTENCE:physical interaction:GO:0031389
Rad17 RFC-like complex [PMID:10660302]"
/note="Checkpoint protein; involved in the activation of
the DNA damage and meiotic pachytene checkpoints; subunit
of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA;
homolog of human and S. pombe Rad17 protein"
/codon_start=1
/product="Rad24p"
/protein_id="NP_011100.1"
/db_xref="GeneID:856920"
/db_xref="SGD:S000000975"
/translation="MDSTNLNKRPLLQYSLSSLGSQITKWSSSRPTSPVRKARSTEND
FLSKQDTSSILPSINDDGGEQWYEKFKPNCLEQVAIHKRKLKDVQEALDAMFLPNAKH
RILLLSGPSGCSKSTVIKELSKILVPKYRQNSNGTSFRSTPNEHKVTEFRGDCIVNDL
PQMESFSEFLKGARYLVMSNLSLILIEDLPNVFHIDTRRRFQQLILQWLYSSEPLLPP
LVICITECEIPENDNNYRKFGIDYTFSAETIMNKEILMHPRLKRIKFNPINSTLLKKH
LKFICVQNMKMLKEKNKWNKRQEVIDYIAQETGDIRSAITTLQFWATSSGSLPISTRE
STISYFHAIGKVIHGSHSTNNDNEMINNLFENSNNLLSKEDFKLGILENYNTFNKGEF
SISDASSIVDCLSECDNMNGLPESNEYGLREVRKTFRNISKQGHNHGTVYFPREWKVR
KLQNSFKVQAEDWLNVSLYKYNAVHSFRNITLEFGYYAPLIRKCQSYKKKYILYYLKN
LPSGSSGPKQTMDKFSDIMKVENGIDVVDRIGGPIEALSVEDGLAPLMDNDSNNCDHL
EDQKKERDRRLRMLIDQYERNVMMANDDLEDEETSFNDDPIVDSDSDNSNNIGNETFG
RSDEDESLCEILSQRQPRKAPVISESLSDSDLEIL"
gene complement(<538434..>539168)
/gene="GRX4"
/locus_tag="YER174C"
/db_xref="GeneID:856921"
mRNA complement(<538434..>539168)
/gene="GRX4"
/locus_tag="YER174C"
/product="monothiol glutaredoxin GRX4"
/transcript_id="NM_001179064.3"
/db_xref="GeneID:856921"
CDS complement(538434..539168)
/gene="GRX4"
/locus_tag="YER174C"
/experiment="EXISTENCE:direct assay:GO:0004364 glutathione
transferase activity [PMID:32098918]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14519092]"
/experiment="EXISTENCE:genetic interaction:GO:0006879
intracellular iron ion homeostasis [PMID:17074835]"
/experiment="EXISTENCE:mutant phenotype:GO:0015036
disulfide oxidoreductase activity
[PMID:10567543|PMID:27085841]"
/experiment="EXISTENCE:mutant phenotype:GO:0030036 actin
cytoskeleton organization [PMID:20889785]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress
[PMID:17074835|PMID:10567543]"
/experiment="EXISTENCE:mutant phenotype:GO:2000678
negative regulation of transcription regulatory region DNA
binding [PMID:23045394]"
/experiment="EXISTENCE:physical interaction:GO:0006879
intracellular iron ion homeostasis [PMID:17074835]"
/experiment="EXISTENCE:physical interaction:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:23045394]"
/note="Glutathione-dependent oxidoreductase and
glutathione S-transferase;
hydroperoxide/superoxide-radical responsive monothiol
glutaredoxin subfamily member with Grx3p and Grx5p;
protects cells from oxidative damage with GRX3 and GRX5;
with Grx3p, promotes dissociation of Aft1p from iron
regulon gene promoters and subsequent nuclear export in
iron-replete conditions, regulating iron homeostasis;
involved with Grx3p in Sir2p deglutathionylation,
restoring deacetylase activity after disulfide stress"
/codon_start=1
/product="monothiol glutaredoxin GRX4"
/protein_id="NP_011101.3"
/db_xref="GeneID:856921"
/db_xref="SGD:S000000976"
/translation="MTVVEIKSQDQFTQLTTTNAANKLIVLYFKAQWADPCKTMSQVL
EAVSEKVRQEDVRFLSIDADEHPEISDLFEIAAVPYFVFIQNGTIVKEISAADPKEFV
KSLEILSNASASLANNAKGPKSTSDEESSGSSDDEEDETEEEINARLVKLVQAAPVML
FMKGSPSEPKCGFSRQLVGILREHQIRFGFFDILRDENVRQSLKKFSDWPTFPQLYIN
GEFQGGLDIIKESIEEDPEYFQHALQ"
gene complement(<539464..>540363)
/gene="TMT1"
/locus_tag="YER175C"
/gene_synonym="TAM1"
/db_xref="GeneID:856922"
mRNA complement(<539464..>540363)
/gene="TMT1"
/locus_tag="YER175C"
/gene_synonym="TAM1"
/product="trans-aconitate 3-methyltransferase"
/transcript_id="NM_001179065.3"
/db_xref="GeneID:856922"
CDS complement(539464..540363)
/gene="TMT1"
/locus_tag="YER175C"
/gene_synonym="TAM1"
/EC_number="2.1.1.145"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:11695919]"
/experiment="EXISTENCE:direct assay:GO:0034198 cellular
response to amino acid starvation [PMID:18092814]"
/experiment="EXISTENCE:direct assay:GO:0046547
trans-aconitate 3-methyltransferase activity
[PMID:11695919]"
/note="Trans-aconitate methyltransferase; cytosolic enzyme
that catalyzes the methyl esterification of
3-isopropylmalate, an intermediate of the leucine
biosynthetic pathway, and trans-aconitate, which inhibits
the citric acid cycle"
/codon_start=1
/product="trans-aconitate 3-methyltransferase"
/protein_id="NP_011102.3"
/db_xref="GeneID:856922"
/db_xref="SGD:S000000977"
/translation="MSTFSASDFNSERYSSSRPSYPSDFYKMIDEYHDGERKLLVDVG
CGPGTATLQMAQELKPFEQIIGSDLSATMIKTAEVIKEGSPDTYKNVSFKISSSDDFK
FLGADSVDKQKIDMITAVECAHWFDFEKFQRSAYANLRKDGTIAIWGYADPIFPDYPE
FDDLMIEVPYGKQGLGPYWEQPGRSRLRNMLKDSHLDPELFHDIQVSYFCAEDVRDKV
KLHQHTKKPLLIRKQVTLVEFADYVRTWSAYHQWKQDPKNKDKEDVADWFIKESLRRR
PELSTNTKIEVVWNTFYKLGKRV"
gene <540650..>540814
/locus_tag="YER175W-A"
/db_xref="GeneID:1466537"
mRNA <540650..>540814
/locus_tag="YER175W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184589.1"
/db_xref="GeneID:1466537"
CDS 540650..540814
/locus_tag="YER175W-A"
/note="hypothetical protein; identified by gene-trapping,
microarray-based expression analysis, and genome-wide
homology searching"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878069.1"
/db_xref="GeneID:1466537"
/db_xref="SGD:S000028625"
/translation="MRLHKTFICFSQNKRGCRNILQENSRMIFENKILIMILRQGIFF
NISVSTKISF"
gene <541690..>545055
/gene="ECM32"
/locus_tag="YER176W"
/gene_synonym="HEL1; MTT1"
/db_xref="GeneID:856923"
mRNA <541690..>545055
/gene="ECM32"
/locus_tag="YER176W"
/gene_synonym="HEL1; MTT1"
/product="RNA helicase"
/transcript_id="NM_001179066.1"
/db_xref="GeneID:856923"
CDS 541690..545055
/gene="ECM32"
/locus_tag="YER176W"
/gene_synonym="HEL1; MTT1"
/EC_number="3.6.4.13"
/experiment="EXISTENCE:direct assay:GO:0003678 DNA
helicase activity [PMID:9434351]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:23222640|PMID:26777405]"
/experiment="EXISTENCE:mutant phenotype:GO:0006449
regulation of translational termination [PMID:10836794]"
/note="DNA dependent ATPase/DNA helicase; helicase
belonging to the Dna2p- and Nam7p-like family of helicases
that is involved in modulating translation termination;
interacts with the translation termination factors,
localized to polysomes"
/codon_start=1
/product="RNA helicase"
/protein_id="NP_011103.1"
/db_xref="GeneID:856923"
/db_xref="SGD:S000000978"
/translation="MDFQCRTCSQAYDAEQMMKHLSSTRHKTVFDTSNDEDICCEECQ
DKNIHQLQIIRFGGEDMVLLCNSCFRKEYSETERPSTSYSLQNGSILKFWEKYVKVRE
CCCDECGEESNLNANRNGEVLCDKCLPKSNRAKDFVSEKSGRFLYIYLGLNETQNSTR
KPRKKGGRRVGRGKKGRKGAKIKKEKKETFEAKISRIAYEVKKENSTIQSSSSSNLRN
FKGFKAVESDPVVAAKVSKSETSRSNPGPSNRNKGKGNKANHKKNSGNGIGKEKERKT
NIRNNVRNSQPIPEDRKNTNSHVTTNSGGKGKNESVDKHQLPQPKALNGNGSGSTNTT
GLKKGKKDHAGQKTKGNDKTGNKNPREAKLNSAGRKNALGKKSNNQPNKGTSRWTIGS
DTESSREPSISPNENTTSITKSRNRNKKASKPTLNEKSKTTTMPKKLETKNQEKNNGK
TKDGKLIYEEGEPLTRYNTFKSTLSYPDLNTYLNDYSFALFLEQKLENEFVQNFNILW
PRNEKDTAFIINVEKNNNSELEKLLPANLLALGRPAFNERQPFFFCTQDEQKVWYIFI
KELSIQRGKYVLLVELFSWNNLSLPTKNGSSQFKLLPTSAQTSRILFAMTRITNPKFI
DLLLGQKPIKEIYFDNRLKFSSDKLNRSQKTAVEHVLNNSITILQGPPGTGKTSTIEE
IIIQVIERFHAFPILCVAASNIAIDNIAEKIMENRPQIKILRILSKKKEQQYSDDHPL
GEICLHNIVYKNLSPDMQVVANKTRRGEMISKSEDTKFYKEKNRVTNKVVSQSQIIFT
TNIAAGGRELKVIKECPVVIMDEATQSSEASTLVPLSLPGIRNFVFVGDEKQLSSFSN
IPQLETSLFERVLSNGTYKNPLMLDTQYRMHPKISEFPIKKIYNGELKDGVTDEQKAW
PGVQHPLFFYQCDLGPESRVRSTQRDIVGFTYENKHECVEIVKIIQILMLDKKVPLEE
IGVITPYSAQRDLLSDILTKNVVINPKQISMQQEYDEIELFNAAGSQGTAGSLQNNVI
NIINGLHVATVDSFQGHEKSFIIFSCVRNNTENKIGFLRDKRRLNVALTRAKHGLIVV
GNKNVLRKGDPLWKDYITYLEEQEVIFTDLTAY"
gene <545611..>546414
/gene="BMH1"
/locus_tag="YER177W"
/gene_synonym="APR6"
/db_xref="GeneID:856924"
mRNA <545611..>546414
/gene="BMH1"
/locus_tag="YER177W"
/gene_synonym="APR6"
/product="14-3-3 family protein BMH1"
/transcript_id="NM_001179067.3"
/db_xref="GeneID:856924"
CDS 545611..546414
/gene="BMH1"
/locus_tag="YER177W"
/gene_synonym="APR6"
/experiment="EXISTENCE:direct assay:GO:0003688 DNA
replication origin binding [PMID:12167636]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12167636|PMID:22842922|PMID:25409301]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:25409301|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405]"
/experiment="EXISTENCE:direct assay:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:24142105]"
/experiment="EXISTENCE:genetic interaction:GO:0001402
signal transduction involved in filamentous growth
[PMID:9215628]"
/experiment="EXISTENCE:genetic interaction:GO:0006031
chitin biosynthetic process [PMID:17559233]"
/experiment="EXISTENCE:genetic interaction:GO:0007124
pseudohyphal growth [PMID:9215628]"
/experiment="EXISTENCE:genetic interaction:GO:0007265 Ras
protein signal transduction [PMID:9215628]"
/experiment="EXISTENCE:genetic interaction:GO:0030437
ascospore formation [PMID:9215628]"
/experiment="EXISTENCE:genetic interaction:GO:0031578
mitotic spindle orientation checkpoint signaling
[PMID:24850890]"
/experiment="EXISTENCE:mutant phenotype:GO:0000077 DNA
damage checkpoint signaling [PMID:14704161]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:24142105]"
/experiment="EXISTENCE:mutant phenotype:GO:0043066
negative regulation of apoptotic process [PMID:22785534]"
/experiment="EXISTENCE:mutant phenotype:GO:0050815
phosphoserine residue binding [PMID:9822578]"
/experiment="EXISTENCE:mutant phenotype:GO:0070842
aggresome assembly [PMID:23843611]"
/experiment="EXISTENCE:physical interaction:GO:0000278
mitotic cell cycle [PMID:17030612]"
/experiment="EXISTENCE:physical interaction:GO:0031397
negative regulation of protein ubiquitination
[PMID:17030612]"
/note="14-3-3 protein, major isoform; involved in
post-transcriptional control of the proteome; binds to
both proteins and to DNA, including replication origins;
regulates exocytosis, vesicle transport, Ras/MAPK and
rapamycin-sensitive signaling, aggresome formation, the
spindle position checkpoint and meiotic commitment;
protein abundance and relative distribution to the nucleus
increase upon DNA replication stress; antiapoptotic gene;
similar to several human 14-3-3 proteins"
/codon_start=1
/product="14-3-3 family protein BMH1"
/protein_id="NP_011104.3"
/db_xref="GeneID:856924"
/db_xref="SGD:S000000979"
/translation="MSTSREDSVYLAKLAEQAERYEEMVENMKTVASSGQELSVEERN
LLSVAYKNVIGARRASWRIVSSIEQKEESKEKSEHQVELICSYRSKIETELTKISDDI
LSVLDSHLIPSATTGESKVFYYKMKGDYHRYLAEFSSGDAREKATNASLEAYKTASEI
ATTELPPTHPIRLGLALNFSVFYYEIQNSPDKACHLAKQAFDDAIAELDTLSEESYKD
STLIMQLLRDNLTLWTSDMSESGQAEDQQQQQQHQQQQPPAAAEGEAPK"
gene <546817..>548079
/gene="PDA1"
/locus_tag="YER178W"
/db_xref="GeneID:856925"
mRNA <546817..>548079
/gene="PDA1"
/locus_tag="YER178W"
/product="pyruvate dehydrogenase (acetyl-transferring)
subunit E1 alpha"
/transcript_id="NM_001179068.3"
/db_xref="GeneID:856925"
CDS 546817..548079
/gene="PDA1"
/locus_tag="YER178W"
/EC_number="1.2.4.1"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:7589446|PMID:24769239|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0006086 pyruvate
decarboxylation to acetyl-CoA [PMID:7947791]"
/experiment="EXISTENCE:direct assay:GO:0042645
mitochondrial nucleoid [PMID:15692048]"
/experiment="EXISTENCE:direct assay:GO:0045254 pyruvate
dehydrogenase complex [PMID:2007123]"
/experiment="EXISTENCE:mutant phenotype:GO:0004739
pyruvate dehydrogenase (acetyl-transferring) activity
[PMID:11589696]"
/experiment="EXISTENCE:mutant phenotype:GO:0007124
pseudohyphal growth [PMID:24603354]"
/note="E1 alpha subunit of the pyruvate dehydrogenase
(PDH) complex; catalyzes the direct oxidative
decarboxylation of pyruvate to acetyl-CoA; phosphorylated;
regulated by glucose; PDH complex is concentrated in spots
within the mitochondrial matrix, often near the ERMES
complex and near peroxisomes"
/codon_start=1
/product="pyruvate dehydrogenase (acetyl-transferring)
subunit E1 alpha"
/protein_id="NP_011105.4"
/db_xref="GeneID:856925"
/db_xref="SGD:S000000980"
/translation="MLAASFKRQPSQLVRGLGAVLRTPTRIGHVRTMATLKTTDKKAP
EDIEGSDTVQIELPESSFESYMLEPPDLSYETSKATLLQMYKDMVIIRRMEMACDALY
KAKKIRGFCHLSVGQEAIAVGIENAITKLDSIITSYRCHGFTFMRGASVKAVLAELMG
RRAGVSYGKGGSMHLYAPGFYGGNGIVGAQVPLGAGLAFAHQYKNEDACSFTLYGDGA
SNQGQVFESFNMAKLWNLPVVFCCENNKYGMGTAASRSSAMTEYFKRGQYIPGLKVNG
MDILAVYQASKFAKDWCLSGKGPLVLEYETYRYGGHSMSDPGTTYRTRDEIQHMRSKN
DPIAGLKMHLIDLGIATEAEVKAYDKSARKYVDEQVELADAAPPPEAKLSILFEDVYV
KGTETPTLRGRIPEDTWDFKKQGFASRD"
gene <548421..>549517
/gene="DMC1"
/locus_tag="YER179W"
/gene_synonym="ISC2"
/db_xref="GeneID:856926"
mRNA join(<548421..548552,548645..>549517)
/gene="DMC1"
/locus_tag="YER179W"
/gene_synonym="ISC2"
/product="recombinase DMC1"
/transcript_id="NM_001179069.1"
/db_xref="GeneID:856926"
CDS join(548421..548552,548645..549517)
/gene="DMC1"
/locus_tag="YER179W"
/gene_synonym="ISC2"
/experiment="EXISTENCE:direct assay:GO:0000150 DNA strand
exchange activity [PMID:23769192]"
/experiment="EXISTENCE:direct assay:GO:0000794 condensed
nuclear chromosome [PMID:1581960]"
/experiment="EXISTENCE:direct assay:GO:0003690
double-stranded DNA binding [PMID:11551925]"
/experiment="EXISTENCE:direct assay:GO:0003697
single-stranded DNA binding [PMID:11551925]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:7528104|PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0042030 ATPase
inhibitor activity [PMID:30301803]"
/experiment="EXISTENCE:genetic interaction:GO:0007130
synaptonemal complex assembly [PMID:24367271]"
/experiment="EXISTENCE:mutant phenotype:GO:0000709 meiotic
joint molecule formation [PMID:9323140|PMID:22955832]"
/experiment="EXISTENCE:mutant phenotype:GO:0007131
reciprocal meiotic recombination
[PMID:1581960|PMID:16581767]"
/experiment="EXISTENCE:mutant phenotype:GO:0051321 meiotic
cell cycle [PMID:1581960]"
/note="Meiosis-specific recombinase required for
double-strand break repair; also required for pairing
between homologous chromosomes and for the normal
morphogenesis of synaptonemal complex; binds ssDNA and
dsDNA, forms helical filaments; potent inhibitor of the
ATPase activity of Srs2p helicase, blocking its ssDNA
translocating motor activity and inhibiting its
antirecombinase activity; stimulated by Rdh54p; homolog of
Rad51p and the bacterial RecA protein"
/codon_start=1
/product="recombinase DMC1"
/protein_id="NP_011106.1"
/db_xref="GeneID:856926"
/db_xref="SGD:S000000981"
/translation="MSVTGTEIDSDTAKNILSVDELQNYGINASDLQKLKSGGIYTVN
TVLSTTRRHLCKIKGLSEVKVEKIKEAAGKIIQVGFIPATVQLDIRQRVYSLSTGSKQ
LDSILGGGIMTMSITEVFGEFRCGKTQMSHTLCVTTQLPREMGGGEGKVAYIDTEGTF
RPERIKQIAEGYELDPESCLANVSYARALNSEHQMELVEQLGEELSSGDYRLIVVDSI
MANFRVDYCGRGELSERQQKLNQHLFKLNRLAEEFNVAVFLTNQVQSDPGASALFASA
DGRKPIGGHVLAHASATRILLRKGRGDERVAKLQDSPDMPEKECVYVIGEKGITDSSD
"
rep_origin 549566..549814
/note="ARS522; Autonomously Replicating Sequence; active
origin of replication near the right end of Chromosome V
that is activated late in S phase, late activation appears
to result from telomeric proximity"
/db_xref="SGD:S000077394"
gene complement(<549724..>550527)
/gene="ISC10"
/locus_tag="YER180C"
/db_xref="GeneID:856927"
mRNA complement(<549724..>550527)
/gene="ISC10"
/locus_tag="YER180C"
/product="Isc10p"
/transcript_id="NM_001179070.1"
/db_xref="GeneID:856927"
CDS complement(549724..550527)
/gene="ISC10"
/locus_tag="YER180C"
/experiment="EXISTENCE:direct assay:GO:0004860 protein
kinase inhibitor activity [PMID:32423992]"
/experiment="EXISTENCE:direct assay:GO:0051019
mitogen-activated protein kinase binding [PMID:32423992]"
/experiment="EXISTENCE:mutant phenotype:GO:0030437
ascospore formation [PMID:8455558]"
/note="Meiosis-specific MAPK inhibitor; binds Smk1p during
meiosis I; links APC/C-Ama1 to Smk1p activation; required
for sporulation; transcript is induced 7.5 hr after
induction of meiosis; Isc10p is ubiquitylated and degraded
in an Ama1p-dependent manner upon completion of meiosis
II"
/codon_start=1
/product="Isc10p"
/protein_id="NP_011107.1"
/db_xref="GeneID:856927"
/db_xref="SGD:S000000982"
/translation="MDVDERLHQDENQTHPFSQKKSSSFLIKEKAATKSKDLEHIRLR
DLNFNHRKKLDDKKLAKQIPVKANFKKPNEIEARANLNSNELTDINLDYIPDSPSIEK
ISGPEDSIVVTPRNIIHLQSDSDIILEECEHNYDCSPFYRLFNYENRIEPDDYEAIIN
AIITDEIAGTYPVFERELEYQELKSLVRKRDYIMYYFLSRDYRGFFQLKEERTLFYRY
PSIAYTSPLRYLDNGSETEQFTGDDDEELQSFDFENTSSVRTLDSNIWR"
gene complement(<550865..>551122)
/gene="SLO1"
/locus_tag="YER180C-A"
/db_xref="GeneID:856928"
mRNA complement(<550865..>551122)
/gene="SLO1"
/locus_tag="YER180C-A"
/product="Slo1p"
/transcript_id="NM_001184517.3"
/db_xref="GeneID:856928"
CDS complement(550865..551122)
/gene="SLO1"
/locus_tag="YER180C-A"
/experiment="EXISTENCE:physical interaction:GO:0006886
intracellular protein transport [PMID:12620189]"
/note="Protein interacting with Arl3p; Arl3p is a GTPase
of the Ras superfamily involved in vesicle-tethering at
the Golgi; putative ortholog of human SCOCO"
/codon_start=1
/product="Slo1p"
/protein_id="NP_796378.3"
/db_xref="GeneID:856928"
/db_xref="SGD:S000028437"
/translation="MSAENISTGSPTGKQPSSEVNLGEREAGTKNERMMRQTKLLKDT
LDLLWNKTLEQQEVCEQLKQENDYLEDYIGNLMRSSNVLEK"
gene complement(551285..551358)
/locus_tag="YNCE0029C"
/db_xref="GeneID:856929"
tRNA complement(551285..551358)
/locus_tag="YNCE0029C"
/product="tRNA-Ile"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Isoleucine tRNA (tRNA-Ile), predicted by
tRNAscan-SE analysis"
/db_xref="GeneID:856929"
/db_xref="SGD:S000006603"
repeat_region 551442..551773
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006921"
gene complement(<551473..>551796)
/locus_tag="YER181C"
/db_xref="GeneID:856930"
mRNA complement(<551473..>551796)
/locus_tag="YER181C"
/product="uncharacterized protein"
/transcript_id="NM_001348828.1"
/db_xref="GeneID:856930"
CDS complement(551473..551796)
/locus_tag="YER181C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/note="Mitochondrial hypothetical protein; conserved among
S. cerevisiae strains; extensively overlaps a Ty1 LTR;
YER181C is not an essential gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335770.1"
/db_xref="GeneID:856930"
/db_xref="SGD:S000000983"
/translation="MLILLRLSEVCVNFVIIIGIPLLIEASILCIQNILELLLKGIGI
LKFNRYLHTIILRLFFLSFYMLHFPITLSILAFQLPLNLLTLSQASFHLPRSHMILYQ
QQECY"
repeat_region complement(552040..552267)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006902"
gene <552525..>553259
/gene="FMP10"
/locus_tag="YER182W"
/db_xref="GeneID:856931"
mRNA <552525..>553259
/gene="FMP10"
/locus_tag="YER182W"
/product="Fmp10p"
/transcript_id="NM_001179072.3"
/db_xref="GeneID:856931"
CDS 552525..553259
/gene="FMP10"
/locus_tag="YER182W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:14576278|PMID:16823961|PMID:24769239|PMID:14562095|P
MID:24390141]"
/note="hypothetical protein; the authentic, non-tagged
protein is detected in highly purified mitochondria in
high-throughput studies"
/codon_start=1
/product="Fmp10p"
/protein_id="NP_011109.3"
/db_xref="GeneID:856931"
/db_xref="SGD:S000000984"
/translation="MFKRIAIAQIRTYTNGVVFKTASKPKRRWIPWTIFGGSFLGGWY
LTQHMTFTDLLAYWRYDALPKNADEVVKYHADLNRRLNGLPIVKQLENAGFVQVIANE
EENLLVSRALNTPGGVAIPPRVYYNPSRRETVGLYHLGMKLTGYPFLIHGGILATVIE
DLMKEAIRLEKGTKNINQETKNLSISYKFPTLANQFVVVRTTDLQQYGNKTKLKAELM
DQSGNRTLVKANATFSSEQGNPKEEK"
gene complement(<553334..>553969)
/gene="FAU1"
/locus_tag="YER183C"
/db_xref="GeneID:856932"
mRNA complement(<553334..>553969)
/gene="FAU1"
/locus_tag="YER183C"
/product="5-formyltetrahydrofolate cyclo-ligase"
/transcript_id="NM_001179073.1"
/db_xref="GeneID:856932"
CDS complement(553334..553969)
/gene="FAU1"
/locus_tag="YER183C"
/EC_number="6.3.3.2"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0009396 folic
acid-containing compound biosynthetic process
[PMID:11923304]"
/experiment="EXISTENCE:direct assay:GO:0030272
5-formyltetrahydrofolate cyclo-ligase activity
[PMID:11923304]"
/note="5,10-methenyltetrahydrofolate synthetase; involved
in folic acid biosynthesis"
/codon_start=1
/product="5-formyltetrahydrofolate cyclo-ligase"
/protein_id="NP_011110.1"
/db_xref="GeneID:856932"
/db_xref="SGD:S000000985"
/translation="MATKQLLRRQIKRVINALDYDIIAAESHTISQAVRSLIASANSR
RVACYMSMDKGEVTTGEIIKNLFQDGQEVFLPRCTHTSESKHFKLREDHHPHLIFHRM
SSLKMVRDLKPQGPYQLKEPEPHIEESDILDVVLVPGVAFDIKTGARMGHGAGYYDDF
FQRYKILHEGQKPLLVGLCLMEQVASPIPLEKHDYSMDCIVCGDGSIHWFQ"
gene complement(<556296..>558680)
/gene="TOG1"
/locus_tag="YER184C"
/db_xref="GeneID:856933"
mRNA complement(<556296..>558680)
/gene="TOG1"
/locus_tag="YER184C"
/product="Tog1p"
/transcript_id="NM_001179074.1"
/db_xref="GeneID:856933"
CDS complement(556296..558680)
/gene="TOG1"
/locus_tag="YER184C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding
[PMID:24998441|PMID:19111667]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:24998441]"
/note="Transcriptional activator of oleate genes;
regulates genes involved in fatty acid utilization; zinc
cluster protein; deletion confers sensitivity to
Calcufluor white, and prevents growth on glycerol or
lactate as sole carbon source"
/codon_start=1
/product="Tog1p"
/protein_id="NP_011111.1"
/db_xref="GeneID:856933"
/db_xref="SGD:S000000986"
/translation="MAAKRGLAKQKSRVTKACDRCHRKKIKCNSKKPCFGCIGSQSKC
TYRNQFREPIEAFFNYTGSLSNDLDNAKCSIAKLKAQLPPSAPASLQKGLANICTELE
KIQPQLYLNLDSKEISSYGGLKSIETEIIGKQSKSLNRFSNAFESNTAQNVSMYFGVY
SPLLYFASTGISWITKKLISCSNDRETRETIYLFLKFLDASSASHAGPKVTSISPLEY
YSKLNGLSCGNDVLIQHIMSNISNEIKGNTNINQTIKFNKPTDWFMYGVQLMEQHHKA
LDRKSSKKLLPLKYFLEQDELIFCLCLEYFERSLFSTMYDLTILKGLVSLMKHRYWID
DPFVLGRIISTMSRRSLDAGLNRWEYYIGQDEDTAEEYRKLWWDCYWWDRWYSLVTGK
QPLIPHEMTSCLFPKDVVGLGVDDSMDCFTLINLVELDPSKFDICISFGYILLTKIIT
AVFSGLLYNRHFTDYRLFATPNAKDLNGTARQLMVEFSKICKIFQCIQDKLIPFLKQY
SENSNVFELYTHFGFAKVCCFQGMESLILRIQNLLQERERIELDSCVKDIRLQTFEAS
VDILTDVLKHEDTFYIFRCSWFIYAILMNITLNFIETPRRNSICYLSLMCRMIASYND
LFVSSGNVNFKGNNAFSKKLENGTAVSFILTRICCQMYTRSQKMAKESLFCELKKYGQ
ACSDAGQAALDIECIWYRNIIGEHKESSFRKEILSILDREMGDLVNNRVIGVQGKNQE
GACYEKLSPSSTSVSVGMDFCSLENFVTAESLPDLLNLFWEDTEFGITKENLGE"
gene <559454..>560365
/gene="PUG1"
/locus_tag="YER185W"
/db_xref="GeneID:856934"
mRNA <559454..>560365
/gene="PUG1"
/locus_tag="YER185W"
/product="Pug1p"
/transcript_id="NM_001179075.3"
/db_xref="GeneID:856934"
CDS 559454..560365
/gene="PUG1"
/locus_tag="YER185W"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:18326586]"
/experiment="EXISTENCE:mutant phenotype:GO:0035351 heme
transmembrane transport [PMID:18326586]"
/note="Plasma membrane protein involved in protoprophyrin
and heme transport; roles in the uptake of protoprophyrin
IX and the efflux of heme; expression is induced under
both low-heme and low-oxygen conditions; member of the
fungal lipid-translocating exporter (LTE) family of
proteins"
/codon_start=1
/product="Pug1p"
/protein_id="NP_011112.3"
/db_xref="GeneID:856934"
/db_xref="SGD:S000000987"
/translation="MSTTDSGFVLYHYTPSKAAAIVFVVLFIIMTVIFAVQTLYAARK
SSKALKNNPFESSDDKVDSLEDAEYKQLKITPTVFAFIPFFTGCIMEAVGYIGRALSS
SNPERTTPYIIQSVLLLVAPALIAATIYMIFGRLLHVMRCQSLILISARFGTTFFVVG
DVFSFFLQAAGGGLMSKAGSTKTGSGLITAGLFVQVIFFGFFIINEIRFTVNVKRRCL
FYEDISRKWIFVNATLLLSSMLILLRSIVRIVEFIQGFNGYIISHEYFIYVFDAVPML
LVIIAFSVGSFFGNVFDVIKECQTLSN"
gene complement(<561705..>562625)
/locus_tag="YER186C"
/db_xref="GeneID:856935"
mRNA complement(<561705..>562625)
/locus_tag="YER186C"
/product="uncharacterized protein"
/transcript_id="NM_001179076.3"
/db_xref="GeneID:856935"
CDS complement(561705..562625)
/locus_tag="YER186C"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011113.3"
/db_xref="GeneID:856935"
/db_xref="SGD:S000000988"
/translation="MLWHPDGYEPRVKAIEEEIYANEDRKDVPDKFKFDTVTKTGMVK
LRVFKDDLIFKSQRSINLFASRKHPFKSFTADGEGLPLFAFRTKKPFFVRRDYVGFLF
YQYEILKNGDFPEESDYEVKGECDGFTLFKVLFCTVKVKKTSYYRNKERISHILELNF
GKKEDFRILTLVRCSEIRSVYVVEDKKVIMKWVFTSESKFNLNSSLFIIKAAIGCLPE
VGDSIEDIPKFDWDSCPTIGCMCRTKEALFQPESRKDMHICPQLFLGETGPPHYNESS
VPWLTKMNICISVLINFLEYTDFMSWMQDN"
repeat_region complement(563928..564024)
/note="Ty5 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006903"
gene <566230..>566655
/locus_tag="YER187W"
/db_xref="GeneID:856937"
mRNA <566230..>566655
/locus_tag="YER187W"
/product="uncharacterized protein"
/transcript_id="NM_001179077.1"
/db_xref="GeneID:856937"
CDS 566230..566655
/locus_tag="YER187W"
/note="hypothetical protein; induced in
respiratory-deficient cells"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011114.1"
/db_xref="GeneID:856937"
/db_xref="SGD:S000000989"
/translation="MLIDQIVAAVPGVPDISGNASALSLQRRSQEFATSWISMTYSQS
YGDLAQNWQNDEGGAGNFDSYAEEKLQNFFSGNRDWKYCFAAEDTKNGEPLDYDDIPG
DGAGTGSAFKSEIYFNTYGGIDNYCNDEHIGAQNTGDGR"
gene <568040..>568759
/locus_tag="YER188W"
/db_xref="GeneID:856938"
mRNA <568040..>568759
/locus_tag="YER188W"
/product="uncharacterized protein"
/transcript_id="NM_001348829.1"
/db_xref="GeneID:856938"
CDS 568040..568759
/locus_tag="YER188W"
/note="hypothetical protein; conserved across S.
cerevisiae strains; large-scale analyses show mRNA
expression increases under anaerobic conditions and
two-hybrid interactions with Sst2p"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335771.1"
/db_xref="GeneID:856938"
/db_xref="SGD:S000000990"
/translation="MMPTYLGKLTWSYFFTTLGLACAYNVTEQMEFDQFKSDYLACLA
PEHRNIVVDLASNGFITISPMANATIDFEDVTSDYFNCTDVNTNVQVTIASFYNEYGF
GPDDNGYYHAMEEPSEFERHDMDVRLYRPYYPGEFVMGRRSDALGVTGFDQKDCAGEG
FYDEQTAATSCQNIGSTQYAKSVRSYNYGCCGGAVWIRIWPHHNCSKGHDHHFKIRPG
QMLCWNVNPYSWMQPESGWNP"
repeat_region 568791..569024
/note="Ty5 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006922"
telomere 569599..576874
/note="TEL05R; Telomeric region on the right arm of
Chromosome V; annotated components include an X element
core sequence, X element combinatorial repeats, and a long
Y' element; TEL05R does have telomeric repeats
(TEL05R-TR), but they are missing from the genome
annotation due to difficulties encountered during
sequencing and/or assembly"
/db_xref="SGD:S000028953"
gene complement(<569608..>569907)
/locus_tag="YER188C-A"
/db_xref="GeneID:1466538"
mRNA complement(<569608..>569907)
/locus_tag="YER188C-A"
/product="uncharacterized protein"
/transcript_id="NM_001184633.1"
/db_xref="GeneID:1466538"
CDS complement(569608..569907)
/locus_tag="YER188C-A"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878070.1"
/db_xref="GeneID:1466538"
/db_xref="SGD:S000028764"
/translation="MAHLSLNQYKCTHIIMHGTCLSGLYPVPFTHKAHDYPHFNIYIS
FGGPKYCITALNTYVIPLFHHLLSTQFIYTYVNITKKSPLKSPKHKNILSFNDNT"
rep_origin 569618..569695
/note="ARS523; Autonomously Replicating Sequence"
/db_xref="SGD:S000077395"
gene <571155..>571523
/locus_tag="YER189W"
/db_xref="GeneID:856939"
mRNA <571155..>571523
/locus_tag="YER189W"
/product="uncharacterized protein"
/transcript_id="NM_001179079.3"
/db_xref="GeneID:856939"
CDS 571155..571523
/locus_tag="YER189W"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011116.3"
/db_xref="GeneID:856939"
/db_xref="SGD:S000000991"
/translation="MKVSDRRKFEKANFDEFESALNNKNDLVHCPSITLFESIPTEVR
SFYEDEKSGLIKVVKFRTGAMDRKRSFEKIVISVMVGKNVQKFLTFVEDEPDFQGGPI
PSNKPRDGLHVVSSAYFEIQ"
gene <571480..>576525
/gene="YRF1-2"
/locus_tag="YER190W"
/gene_synonym="YRF1"
/db_xref="GeneID:856940"
mRNA <571480..>576525
/gene="YRF1-2"
/locus_tag="YER190W"
/gene_synonym="YRF1"
/product="Y' element ATP-dependent helicase protein 1 copy
2"
/transcript_id="NM_001179080.3"
/db_xref="GeneID:856940"
CDS 571480..576525
/gene="YRF1-2"
/locus_tag="YER190W"
/gene_synonym="YRF1"
/experiment="EXISTENCE:curator inference:GO:0005634
nucleus [PMID:9837911]"
/experiment="EXISTENCE:direct assay:GO:0003678 DNA
helicase activity [PMID:9837911]"
/experiment="EXISTENCE:genetic interaction:GO:0000722
telomere maintenance via recombination [PMID:9837911]"
/note="Helicase encoded by the Y' element of subtelomeric
regions; highly expressed in the mutants lacking the
telomerase component TLC1; potentially phosphorylated by
Cdc28p; induced by treatment with 8-methoxypsoralen and
UVA irradiation"
/codon_start=1
/product="Y' element ATP-dependent helicase protein 1 copy
2"
/protein_id="NP_011117.3"
/db_xref="GeneID:856940"
/db_xref="SGD:S000000992"
/translation="MVYTLFQVHTLKFNRKDYDTLSLFYLNRGYYNELSFRVLERCHE
KASARPNDSSTMRTFTDFVSGAPIVRSLQKSTIRKYGYNLAPYMFLLLHVDELSIFSA
YQASLPGEKKVDTERLKRDLCPRKPTEIKYFSQICNDMMNKKDRLGDILHIILRACAL
NFGAGPRGGAGDEEDRSITNEEPIIPSVDEHGLKVCKLRSPNTPRRLRKTLDAVKALL
VSSCACTARDLDIFDDNNGVAMWKWIKILYHEVAQETALKDSYRITLVPSSDGVSVCG
KLFNREYVRGFYFACKAQFDNLWEELNDCFYMPTVVDIASLILRNREVLFREPKRGID
EYLENDSFLQMIPVKYREIVLPKLRRDTNKMTAALKNKVTVAIDELTVPLMWMIHFAV
GYPYRYPELQLLAFAGPQRNVYVDDTTRRIQLYTDYNKNGSSEPRLKTLDGLTSDYVF
YFVTVLRQMQICALGNSYDAFNHDPWMDVVGFEDPDQVTNRDISRIVLYSYMFLNTAK
GCLVEYATFRQYMRELPKNAPQKLNFREMRQGLIALGRHCVGSRFETDLYESATSELM
ANHSVQTGRNIYGVDSFSLTSVSGTTATLLQERASERWIQWLGLESDYHCSFSSTRNA
EDVVAGEAASSDHHQKISRVTRKRPREPKSTNDILVAGQKLFGSSFEFRDLHQLRLCH
EIYMADTPSVAVQAPPGYGKTELFHLPLIALASKGDVKYVSFLFVPYTVLLANCMIRL
SRCGCLNVAPVRNFIEEGCDGVTDLYVGIYDDLASTNFTDRIAAWENIVECTFRTNNV
KLGYLIVDEFHNFETEVYRQSQFGGITNLDFDAFEKAIFLSGTAPEAVADAALQRIGL
TGLAKKSMDINELKRSEDLSRGLSSYPTRMFNLIKEKSEVPLGHVHKIWKKVESQPEE
ALKLLLALFEIEPESKAIVVASTTNEVEELACSWRKYFRVVWIHGKLGAAEKVSRTKE
FVTDGSMRVLIGTKLVTEGIDIKQLMMVIMLDNRLNIIELIQGVGRLRDGGLCYLLSR
KNSWAARNRKGELPPIKEGCITEQVREFYGLESKKGKKGQHVGCCGSRTDLSADTVEL
IERMDRLAEKQATASMSIVALPSSFQESNSSDRCRKYCSSDEDSDTCIHGSANASTNA
TTNSSTNATTTASTNVRTSATTTASINVRTSATTTESTNSSTNATTTASTNVRTSATT
TASINVRTSATTTESTNSNTSATTTESTDSNTSATTTESTDSNTSATTTASTNSSTNA
TTTASTNSSTNATTTESTNASAKEDANKDGNAEDNRFHPVTDINKESYKRKGSQMVLL
ERKKLKAQFPNTSENMNVLQFLGFRSDEIKHLFLYGIDVYFCPEGVFTQYGLCKGCQK
MFELCVCWAGQKVSYRRMAWEALAVERMLRNDEEYKEYLEDIEPYHGDPVGYLKYFSV
KRGEIYSQIQRNYAWYLAITRRRETISVLDSTRGKQGSQVFRMSGRQIKELYYKVWSN
LRESKTEVLQYFLNWDEKKCREEWEAKDDTVFVEALEKVGVFQRLRSMTSAGLQGPQY
VKLQFSRHHRQLRSRYELSLGMHLRDQLALGVTPSKVPHWTAFLSMLIGLFCNKTFRQ
KLEYLLEQISEVWLLPHWLDLANVEVLAADNTRVPLYMLMVAVHKELDSDDVPDGRFD
ILLCRDSSREVGE"
gene complement(<575680..>576162)
/locus_tag="YER190C-B"
/db_xref="GeneID:4594642"
mRNA complement(<575680..>576162)
/locus_tag="YER190C-B"
/product="uncharacterized protein"
/transcript_id="NM_001184590.1"
/db_xref="GeneID:4594642"
CDS complement(575680..576162)
/locus_tag="YER190C-B"
/note="hypothetical protein; identified by gene-trapping,
microarray-based expression analysis, and genome-wide
homology searching"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001073292.1"
/db_xref="GeneID:4594642"
/db_xref="SGD:S000028627"
/translation="MMPAKLQLDVLRTLQSSARHGTQTLKNSNFLERFHKDRIVFCLP
FFPALFFVPVQKVLQHLCLRFTQVAPYFIIQLFDLPSRHAENLAPLLASCRIQYTNCF
SSSSNGQVPSIISLYLRVDLSPFYAKIFQISYRVPMIWLDVFQVFFVFLVISQHSLHS
"
CONTIG join(BK006939.2:1..576874)
//
