LOCUS NC_001139 1090940 bp DNA linear CON 23-JAN-2026
DEFINITION Saccharomyces cerevisiae S288C chromosome VII, complete sequence.
ACCESSION NC_001139
VERSION NC_001139.9
DBLINK BioProject: PRJNA128
Assembly: GCF_000146045.2
KEYWORDS RefSeq.
SOURCE Saccharomyces cerevisiae S288C
ORGANISM Saccharomyces cerevisiae S288C
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
Saccharomyces.
REFERENCE 1 (bases 1 to 1090940)
AUTHORS Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M.,
Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S.,
Weng,S. and Cherry,J.M.
TITLE New data and collaborations at the Saccharomyces Genome Database:
updated reference genome, alleles, and the Alliance of Genome
Resources
JOURNAL Genetics 220 (4) (2022)
PUBMED 34897464
REFERENCE 2 (bases 1 to 1090940)
AUTHORS Tettelin,H., Agostoni Carbone,M.L., Albermann,K., Albers,M.,
Arroyo,J., Backes,U., Barreiros,T., Bertani,I., Bjourson,A.J.,
Bruckner,M., Bruschi,C.V., Carignani,G., Castagnoli,L., Cerdan,E.,
Clemente,M.L., Coblenz,A., Coglievina,M., Coissac,E., Defoor,E.,
Del Bino,S., Delius,H., Delneri,D., de Wergifosse,P., Dujon,B.,
Kleine,K. et al.
TITLE The nucleotide sequence of Saccharomyces cerevisiae chromosome VII
JOURNAL Nature 387 (6632 SUPPL), 81-84 (1997)
PUBMED 9169869
REFERENCE 3 (bases 1 to 1090940)
AUTHORS Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
Oliver,S.G.
TITLE Life with 6000 genes
JOURNAL Science 274 (5287), 546 (1996)
PUBMED 8849441
REFERENCE 4 (bases 1 to 1090940)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (23-JAN-2026) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 5 (bases 1 to 1090940)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (16-JAN-2015) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Protein update by submitter
REFERENCE 6 (bases 1 to 1090940)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (04-MAY-2012) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Protein update by submitter
REFERENCE 7 (bases 1 to 1090940)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (31-MAR-2011) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Sequence update by submitter
REFERENCE 8 (bases 1 to 1090940)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (11-DEC-2009) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
COMMENT REVIEWED REFSEQ: This record has been curated by SGD. The reference
sequence is identical to BK006941.
On Apr 26, 2011 this sequence version replaced NC_001139.8.
##Genome-Annotation-Data-START##
Annotation Provider :: SGD
Annotation Status :: Full Annotation
Annotation Version :: R64-4-1
URL :: http://www.yeastgenome.org/
##Genome-Annotation-Data-END##
COMPLETENESS: full length.
FEATURES Location/Qualifiers
source 1..1090940
/organism="Saccharomyces cerevisiae S288C"
/mol_type="genomic DNA"
/strain="S288C"
/db_xref="taxon:559292"
/chromosome="VII"
telomere complement(1..781)
/note="TEL07L; Telomeric region on the left arm of
Chromosome VII; composed of an X element core sequence, X
element combinatorial repeats, and a short terminal
stretch of telomeric repeats"
/db_xref="SGD:S000028887"
repeat_region 838..1079
/note="Ty5 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006948"
gene <2790..>3932
/gene="COS12"
/locus_tag="YGL263W"
/db_xref="GeneID:852628"
mRNA <2790..>3932
/gene="COS12"
/locus_tag="YGL263W"
/product="Cos12p"
/transcript_id="NM_001181129.1"
/db_xref="GeneID:852628"
CDS 2790..3932
/gene="COS12"
/locus_tag="YGL263W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/note="Endosomal protein involved in turnover of plasma
membrane proteins; member of the DUP380 subfamily of
conserved, often subtelomeric COS genes; required for the
multivesicular vesicle body sorting pathway that
internalizes plasma membrane proteins for degradation; Cos
proteins provide ubiquitin in trans for nonubiquitinated
cargo proteins"
/codon_start=1
/product="Cos12p"
/protein_id="NP_011251.1"
/db_xref="GeneID:852628"
/db_xref="SGD:S000003232"
/translation="MDGAKFENTVAFLPSEIFDCYNSTLPKNVFRSFVTWSCYEKFNS
LEFRTWLLMWLPLIIAWKIRGKRHYLVIVTALMFEVLYFLWTYSYIFRERTLGKQVSQ
FAKEIITNTPGIDTEDWERVAVNFNSYLYENKLWNTEYFFFDGSSCQEAFRKMLLEPF
SLKKNDFANAKVPDGSVCYTEKALQVYFTQIERKWHWINSEGFLHNKTTQSVQFSKHG
YGSKLLWAFKEVTIMNSRFAFFSIAYLNGLLTIPRLRNSLHILYVCAVLSSMIIEYLI
GIDKFRFKSMNLIHKLQFLSYITCGHEKSDATNWSQIAKRTNTYMFEQKIWNSPILFS
DGIDCEKFFKWYFSTPVSSQASLSVGSTDFELWPYIKEAQSACNDV"
gene <5312..>5839
/locus_tag="YGL262W"
/db_xref="GeneID:852629"
mRNA <5312..>5839
/locus_tag="YGL262W"
/product="uncharacterized protein"
/transcript_id="NM_001181128.1"
/db_xref="GeneID:852629"
CDS 5312..5839
/locus_tag="YGL262W"
/note="hypothetical protein; null mutant displays elevated
sensitivity to expression of a mutant huntingtin fragment
or of alpha-synuclein; YGL262W is not an essential gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011252.1"
/db_xref="GeneID:852629"
/db_xref="SGD:S000003231"
/translation="MRNNVTELVNSIIGVQTPGSLPDTLSGAHSLQRRISYFDVNWIS
WNWDNVNVDLNKEVKKSRPLLGEEDDQCMFGWFANNPGWKYYWSVTDNPDPGYKENYS
DIGDENAVHGELYFNTYGGLMASVMTTKMVLNAKRQLVVIDTIVVKAICDYVMKYWKK
KVNLTTISLYLMLKL"
gene complement(<6290..>6652)
/gene="PAU11"
/locus_tag="YGL261C"
/db_xref="GeneID:852630"
mRNA complement(<6290..>6652)
/gene="PAU11"
/locus_tag="YGL261C"
/product="seripauperin PAU11"
/transcript_id="NM_001181127.1"
/db_xref="GeneID:852630"
CDS complement(6290..6652)
/gene="PAU11"
/locus_tag="YGL261C"
/note="hypothetical protein; member of the seripauperin
multigene family encoded mainly in subtelomeric regions;
mRNA expression appears to be regulated by SUT1 and UPC2"
/codon_start=1
/product="seripauperin PAU11"
/protein_id="NP_011253.1"
/db_xref="GeneID:852630"
/db_xref="SGD:S000003230"
/translation="MVKLTSIAAGVAAIAATASATTTLAQSDERVNLVELGVYVSDIR
AHLAQYYMFQAAHPTETYPVEVAEAVFNYGDFTTMLTGIAPDQVTRMITGVPWYSSRL
KPAISSALSKDGIYTIAN"
gene <6860..>7090
/locus_tag="YGL260W"
/db_xref="GeneID:852631"
mRNA <6860..>7090
/locus_tag="YGL260W"
/product="uncharacterized protein"
/transcript_id="NM_001181126.1"
/db_xref="GeneID:852631"
CDS 6860..7090
/locus_tag="YGL260W"
/note="hypothetical protein; transcription is
significantly increased in a NAP1 deletion background;
deletion mutant has increased accumulation of nickel and
selenium"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011254.1"
/db_xref="GeneID:852631"
/db_xref="SGD:S000003229"
/translation="MEMLLFLNESYIFHRLRMWSIVLWHSCVFVCAECGNANYRVPRC
LIKPFSVPVTFPFSVKKNIRILDLDPRTEAYC"
gene <8470..>8967
/gene="YPS5"
/locus_tag="YGL259W"
/db_xref="GeneID:852632"
mRNA <8470..>8967
/gene="YPS5"
/locus_tag="YGL259W"
/product="Yps5p"
/transcript_id="NM_001181125.1"
/db_xref="GeneID:852632"
CDS 8470..8967
/gene="YPS5"
/locus_tag="YGL259W"
/note="Protein with similarity to GPI-anchored aspartic
proteases; such proteases are Yap1p and Yap3p; mCherry
fusion protein localizes to the vacuole"
/codon_start=1
/product="Yps5p"
/protein_id="NP_011255.1"
/db_xref="GeneID:852632"
/db_xref="SGD:S000003228"
/translation="MQLFSILSLLSSLMCSLTVLGSSASSYVKFPVQKFADIINIGTQ
DVSTVFKRNEVLNTTVINGIGVYVVKMEIGTPPQTVYLQLDTGSSDMIVNNADIAYCK
SMSDGSDYASTDNYELTATFTGPRSTTTSPELITLSALIGVNSMQETHLLLRITRLSS
MTYTY"
gene <9162..>9395
/locus_tag="YGL258W-A"
/db_xref="GeneID:852633"
mRNA <9162..>9395
/locus_tag="YGL258W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184473.1"
/db_xref="GeneID:852633"
CDS 9162..9395
/locus_tag="YGL258W-A"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_076890.1"
/db_xref="GeneID:852633"
/db_xref="SGD:S000007607"
/translation="MAFERQGKIEKKISYSLFLNGPNVHFGSILFGAVDKSKYAEELC
THPMRQAYNTLDSNSRIIITVQSVAILDGKLVW"
gene <11110..>11730
/gene="VEL1"
/locus_tag="YGL258W"
/db_xref="GeneID:852634"
mRNA <11110..>11730
/gene="VEL1"
/locus_tag="YGL258W"
/product="Vel1p"
/transcript_id="NM_001181124.1"
/db_xref="GeneID:852634"
CDS 11110..11730
/gene="VEL1"
/locus_tag="YGL258W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:11935221]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="hypothetical protein; highly induced in
zinc-depleted conditions and has increased expression in
NAP1 deletion mutants; VEL1 has a paralog, YOR387C, that
arose from a single-locus duplication"
/codon_start=1
/product="Vel1p"
/protein_id="NP_011256.1"
/db_xref="GeneID:852634"
/db_xref="SGD:S000003227"
/translation="MSFLSIFTFFSVLISVATTVRFDLTNVTCKGLHGPHCGTYVMEV
VGQNGTFLGQSTFVGADVLTESAGDAWARYLGQETRFLPKLTTIASNETKNFSPLIFT
TNINTCNPQSIGDAMVPFANTVTGEIEYNSWADTADNASFITGLANQLFNSTDYGVQV
ASCYPNFASVILSTPAVNIFGKDDTLPDYCTAIQLKAVCPPEAGFD"
gene complement(<12481..>14157)
/gene="MNT2"
/locus_tag="YGL257C"
/db_xref="GeneID:852635"
mRNA complement(<12481..>14157)
/gene="MNT2"
/locus_tag="YGL257C"
/product="alpha-1,3-mannosyltransferase MNT2"
/transcript_id="NM_001181123.1"
/db_xref="GeneID:852635"
CDS complement(12481..14157)
/gene="MNT2"
/locus_tag="YGL257C"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:30700649]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0000033
alpha-1,3-mannosyltransferase activity [PMID:10521541]"
/experiment="EXISTENCE:genetic interaction:GO:0006493
protein O-linked glycosylation [PMID:10521541]"
/experiment="EXISTENCE:mutant phenotype:GO:0000033
alpha-1,3-mannosyltransferase activity [PMID:10521541]"
/experiment="EXISTENCE:mutant phenotype:GO:0006493 protein
O-linked glycosylation [PMID:10521541]"
/note="Mannosyltransferase; involved in adding the 4th and
5th mannose residues of O-linked glycans"
/codon_start=1
/product="alpha-1,3-mannosyltransferase MNT2"
/protein_id="NP_011257.1"
/db_xref="GeneID:852635"
/db_xref="SGD:S000003226"
/translation="MRRKNRLFILVVLLGIVLVVYYSQLNSLDLVEPVQSSSSGNGGC
WSYYEGLTPGWLNDFYDVNQITPNPAKDVIELVTRIKIFFNCLQQVDGHNIQRLRDIE
KKLFPYINFEKLETDESAFWHTTTRWNGEVYHASMLEFDPKNHQFLRSKPINFDTGLS
FWENWLHTVTQSGSKGIVISASDVQLNETIRLLKVLRFIKNDYPIQIVHNADLSQDSM
KSIIKYARSLDTAEYPAQELWFLNVHSLLNPKYSKKFTTYSNKWLALTFSSFEIPILM
DSDTVPFVSIEKFYELEEFQKTGVLFFKDRVISDDLFESSELKILREIVYGCIGLDLE
DESKIHEQVEDPVVAQVLENMFIKKYKHHLESGLVILHKGKHLFSMLTSIALQFSPIA
EYFHGDKDFFWLGELLSNNRFTFHPVDASNIGQLGNVVSKESTGEFYQICSVQLSHTD
RDGSLLWLNGGLNICKKTSWEYDYEHRQRLNDMFQNADELREYYASPVKLEGIIIPDT
SISGWINSGECFLFNYCTLFKEGEFGKLIKFKEDEKLRLSQIVDIWNKDI"
gene <15159..>16307
/gene="ADH4"
/locus_tag="YGL256W"
/gene_synonym="NRC465; ZRG5"
/db_xref="GeneID:852636"
mRNA <15159..>16307
/gene="ADH4"
/locus_tag="YGL256W"
/gene_synonym="NRC465; ZRG5"
/product="alcohol dehydrogenase ADH4"
/transcript_id="NM_001181122.1"
/db_xref="GeneID:852636"
CDS 15159..16307
/gene="ADH4"
/locus_tag="YGL256W"
/gene_synonym="NRC465; ZRG5"
/EC_number="1.1.1.1"
/experiment="EXISTENCE:direct assay:GO:0004022 alcohol
dehydrogenase (NAD+) activity [PMID:3282541]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:14562095|PMID:17054397]"
/experiment="EXISTENCE:direct assay:GO:0006066 alcohol
metabolic process [PMID:38803372]"
/experiment="EXISTENCE:genetic interaction:GO:0000947
amino acid catabolic process to alcohol via Ehrlich
pathway [PMID:12499363]"
/experiment="EXISTENCE:mutant phenotype:GO:0004022 alcohol
dehydrogenase (NAD+) activity [PMID:3023838]"
/experiment="EXISTENCE:mutant phenotype:GO:0006113
fermentation [PMID:3023838]"
/note="Alcohol dehydrogenase isoenzyme type IV; dimeric
enzyme demonstrated to be zinc-dependent despite sequence
similarity to iron-activated alcohol dehydrogenases;
transcription is induced in response to zinc deficiency"
/codon_start=1
/product="alcohol dehydrogenase ADH4"
/protein_id="NP_011258.2"
/db_xref="GeneID:852636"
/db_xref="SGD:S000003225"
/translation="MSSVTGFYIPPISFFGEGALEETADYIKNKDYKKALIVTDPGIA
AIGLSGRVQKMLEERDLNVAIYDKTQPNPNIANVTAGLKVLKEQNSEIVVSIGGGSAH
DNAKAIALLATNGGEIGDYEGVNQSKKAALPLFAINTTAGTASEMTRFTIISNEEKKI
KMAIIDNNVTPAVAVNDPSTMFGLPPALTAATGLDALTHCIEAYVSTASNPITDACAL
KGIDLINESLVAAYKDGKDKKARTDMCYAEYLAGMAFNNASLGYVHALAHQLGGFYHL
PHGVCNAVLLPHVQEANMQCPKAKKRLGEIALHFGASQEDPEETIKALHVLNRTMNIP
RNLKELGVKTEDFEILAEHAMHDACHLTNPVQFTKEQVVAIIKKAYEY"
gene complement(17213..17709)
/gene="SUT532"
/locus_tag="YNCG0047C"
/db_xref="GeneID:84313894"
ncRNA complement(17213..17709)
/ncRNA_class="other"
/gene="SUT532"
/locus_tag="YNCG0047C"
/product="SUT532"
/note="Stable Unannotated Transcript functional ncRNA;
acts in trans to regulate gene expression by modulating
transcription factors"
/transcript_id="NR_188205.1"
/db_xref="GeneID:84313894"
/db_xref="SGD:S000345040"
gene <20978..>22108
/gene="ZRT1"
/locus_tag="YGL255W"
/db_xref="GeneID:852637"
mRNA <20978..>22108
/gene="ZRT1"
/locus_tag="YGL255W"
/product="high-affinity Zn(2+) transporter ZRT1"
/transcript_id="NM_001181121.1"
/db_xref="GeneID:852637"
CDS 20978..22108
/gene="ZRT1"
/locus_tag="YGL255W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0000006
high-affinity zinc transmembrane transporter activity
[PMID:8637895]"
/experiment="EXISTENCE:mutant phenotype:GO:0005886 plasma
membrane [PMID:8637895]"
/experiment="EXISTENCE:mutant phenotype:GO:0071578 zinc
ion import across plasma membrane [PMID:8637895]"
/note="High-affinity zinc transporter of the plasma
membrane; responsible for the majority of zinc uptake;
transcription is induced under low-zinc conditions by the
Zap1p transcription factor"
/codon_start=1
/product="high-affinity Zn(2+) transporter ZRT1"
/protein_id="NP_011259.1"
/db_xref="GeneID:852637"
/db_xref="SGD:S000003224"
/translation="MSNVTTPWWKQWDPSEVTLADKTPDDVWKTCVLQGVYFGGNEYN
GNLGARISSVFVILFVSTFFTMFPLISTKVKRLRIPLYVYLFAKYFGSGVIVATAFIH
LMDPAYGAIGGTTCVGQTGNWGLYSWCPAIMLTSLTFTFLTDLFSSVWVERKYGLSHD
HTHDEIKDTVVRNTAAVSSENDNENGTANGSHDTKNGVEYYEDSDATSMDVVQSFQAQ
FYAFLILEFGVIFHSVMIGLNLGSVGDEFSSLYPVLVFHQSFEGLGIGARLSAIEFPR
SKRWWPWALCVAYGLTTPICVAIGLGVRTRYVSGSYTALVISGVLDAISAGILLYTGL
VELLARDFIFNPQRTKDLRELSFNVICTLFGAGIMALIGKWA"
gene <22304..>23203
/gene="FZF1"
/locus_tag="YGL254W"
/gene_synonym="NRC299; RSU1; SUL1"
/db_xref="GeneID:852638"
mRNA <22304..>23203
/gene="FZF1"
/locus_tag="YGL254W"
/gene_synonym="NRC299; RSU1; SUL1"
/product="Fzf1p"
/transcript_id="NM_001181120.1"
/db_xref="GeneID:852638"
CDS 22304..23203
/gene="FZF1"
/locus_tag="YGL254W"
/gene_synonym="NRC299; RSU1; SUL1"
/experiment="EXISTENCE:curator inference:GO:0005634
nucleus [PMID:10234785]"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:35809138|PMID:10234785]"
/experiment="EXISTENCE:direct assay:GO:0001228 DNA-binding
transcription activator activity, RNA polymerase
II-specific [PMID:10234785]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:19111667]"
/experiment="EXISTENCE:mutant phenotype:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:10234785]"
/experiment="EXISTENCE:mutant phenotype:GO:0001228
DNA-binding transcription activator activity, RNA
polymerase II-specific [PMID:10234785]"
/experiment="EXISTENCE:mutant phenotype:GO:0018890
cyanamide metabolic process [PMID:35809138]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:35809138|PMID:10870099]"
/note="Transcription factor involved in sulfite
metabolism; activates transcription of SSU1, DDI2, DDI3;
overexpression suppresses sulfite-sensitivity of many
unrelated mutants due to hyperactivation of SSU1, contains
five zinc fingers; protein abundance increases in response
to DNA replication stress"
/codon_start=1
/product="Fzf1p"
/protein_id="NP_011260.1"
/db_xref="GeneID:852638"
/db_xref="SGD:S000003223"
/translation="MTDIGRTKSRNYKCSFDGCEKVYNRPSLLQQHQNSHTNQKPYHC
DEPGCGKKFIRPCHLRVHKWTHSQIKPKACTLCQKRFVTNQQLRRHLNSHERKSKLAS
RIDRKHEGVNANVKAELNGKEGGFDPKLPSGSPMCGEEFSQGHLPGYDDMQVLQCPYK
SCQKVTSFNDDLINHMLQHHIASKLVVPSGDPSLKESLPTSEKSSSTDTTSIPQLSFS
TTGTSSSESVDSTTAQTPTDPESYWSDNRCKHSDCQELSPFASVFDLIDHYDHTHAFI
PETLVKYSYIHLYKPSVWDLFEY"
gene <23935..>25395
/gene="HXK2"
/locus_tag="YGL253W"
/gene_synonym="HEX1; HKB; SCI2"
/db_xref="GeneID:852639"
mRNA <23935..>25395
/gene="HXK2"
/locus_tag="YGL253W"
/gene_synonym="HEX1; HKB; SCI2"
/product="hexokinase 2"
/transcript_id="NM_001181119.1"
/db_xref="GeneID:852639"
CDS 23935..25395
/gene="HXK2"
/locus_tag="YGL253W"
/gene_synonym="HEX1; HKB; SCI2"
/EC_number="2.7.1.1"
/experiment="EXISTENCE:direct assay:GO:0004396 hexokinase
activity [PMID:12611889|PMID:332086]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9563516|PMID:37196001|PMID:11311123]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16962558]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:9563516|PMID:11311123]"
/experiment="EXISTENCE:direct assay:GO:0006013 mannose
metabolic process [PMID:332086]"
/experiment="EXISTENCE:direct assay:GO:0006096 glycolytic
process [PMID:8917466|PMID:332086]"
/experiment="EXISTENCE:direct assay:GO:0046015 regulation
of transcription by glucose [PMID:11311123]"
/experiment="EXISTENCE:genetic interaction:GO:0046323
D-glucose import [PMID:6300872]"
/experiment="EXISTENCE:genetic interaction:GO:1990539
fructose import across plasma membrane [PMID:6300872]"
/experiment="EXISTENCE:mutant phenotype:GO:0004396
hexokinase activity [PMID:6341351]"
/experiment="EXISTENCE:mutant phenotype:GO:0006000
fructose metabolic process [PMID:6300872]"
/experiment="EXISTENCE:mutant phenotype:GO:0006006 glucose
metabolic process [PMID:6300872]"
/experiment="EXISTENCE:mutant phenotype:GO:0006096
glycolytic process [PMID:8917466]"
/experiment="EXISTENCE:mutant phenotype:GO:0008361
regulation of cell size [PMID:12089449]"
/experiment="EXISTENCE:mutant phenotype:GO:0031966
mitochondrial membrane [PMID:31862471]"
/experiment="EXISTENCE:mutant phenotype:GO:2001234
negative regulation of apoptotic signaling pathway
[PMID:31862471]"
/note="Hexokinase isoenzyme 2; phosphorylates glucose in
cytosol; predominant hexokinase during growth on glucose;
represses expression of HXK1, GLK1, induces expression of
its own gene; antiapoptotic;
phosphorylation/dephosphorylation at Ser14 by kinase
Snf1p, phosphatase Glc7p-Reg1p regulates nucleocytoplasmic
shuttling of Hxk2p; functions downstream of Sit4p in
control of cell cycle, mitochondrial function, oxidative
stress resistance, chronological lifespan; has paralog
HXK1"
/codon_start=1
/product="hexokinase 2"
/protein_id="NP_011261.1"
/db_xref="GeneID:852639"
/db_xref="SGD:S000003222"
/translation="MVHLGPKKPQARKGSMADVPKELMQQIENFEKIFTVPTETLQAV
TKHFISELEKGLSKKGGNIPMIPGWVMDFPTGKESGDFLAIDLGGTNLRVVLVKLGGD
RTFDTTQSKYRLPDAMRTTQNPDELWEFIADSLKAFIDEQFPQGISEPIPLGFTFSFP
ASQNKINEGILQRWTKGFDIPNIENHDVVPMLQKQITKRNIPIEVVALINDTTGTLVA
SYYTDPETKMGVIFGTGVNGAYYDVCSDIEKLQGKLSDDIPPSAPMAINCEYGSFDNE
HVVLPRTKYDITIDEESPRPGQQTFEKMSSGYYLGEILRLALMDMYKQGFIFKNQDLS
KFDKPFVMDTSYPARIEEDPFENLEDTDDLFQNEFGINTTVQERKLIRRLSELIGARA
ARLSVCGIAAICQKRGYKTGHIAADGSVYNRYPGFKEKAANALKDIYGWTQTSLDDYP
IKIVPAEDGSGAGAAVIAALAQKRIAEGKSVGIIGA"
gene complement(<25718..>27484)
/gene="RTG2"
/locus_tag="YGL252C"
/db_xref="GeneID:852640"
mRNA complement(<25718..>27484)
/gene="RTG2"
/locus_tag="YGL252C"
/product="Rtg2p"
/transcript_id="NM_001181118.1"
/db_xref="GeneID:852640"
CDS complement(25718..27484)
/gene="RTG2"
/locus_tag="YGL252C"
/experiment="EXISTENCE:direct assay:GO:0000791 euchromatin
[PMID:12446794]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:10848632]"
/experiment="EXISTENCE:direct assay:GO:0045944 positive
regulation of transcription by RNA polymerase II
[PMID:7493987]"
/experiment="EXISTENCE:direct assay:GO:0051219
phosphoprotein binding [PMID:14536080]"
/experiment="EXISTENCE:genetic interaction:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:7493987]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:11687605|PMID:8422683]"
/experiment="EXISTENCE:mutant phenotype:GO:0006606 protein
import into nucleus [PMID:11076970]"
/experiment="EXISTENCE:mutant phenotype:GO:0031930
mitochondria-nucleus signaling pathway
[PMID:7493987|PMID:8422683]"
/experiment="EXISTENCE:mutant phenotype:GO:0034504 protein
localization to nucleus [PMID:10848632]"
/experiment="EXISTENCE:mutant phenotype:GO:0035753
maintenance of DNA trinucleotide repeats [PMID:12399373]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:7493987]"
/note="Sensor of mitochondrial dysfunction; regulates the
subcellular location of Rtg1p and Rtg3p, transcriptional
activators of the retrograde (RTG) and TOR pathways; Rtg2p
is inhibited by the phosphorylated form of Mks1p"
/codon_start=1
/product="Rtg2p"
/protein_id="NP_011262.1"
/db_xref="GeneID:852640"
/db_xref="SGD:S000003221"
/translation="MSTLSDSDTETEVVSRNLCGIVDIGSNGIRFSISSKAAHHARIM
PCVFKDRVGLSLYEVQYNTHTNAKCPIPRDIIKEVCSAMKRFKLICDDFGVPETSVRV
IATEATRDAINADEFVNAVYGSTGWKVEILGQEDETRVGIYGVVSSFNTVRGLYLDVA
GGSTQLSWVISSHGEVKQSSKPVSLPYGAGTLLRRMRTDDNRALFYEIKEAYKDAIEK
IGIPQEMIDDAKKEGGFDLWTRGGGLRGMGHLLLYQSEGYPIQTIINGYACTYEEFSS
MSDYLFLKQKIPGSSKEHKIFKVSDRRALQLPAVGLFMSAVFEAIPQIKAVHFSEGGV
REGSLYSLLPKEIRAQDPLLIASRPYAPLLTEKYLYLLRTSIPQEDIPEIVNERIAPA
LCNLAFVHASYPKELQPTAALHVATRGIIAGCHGLSHRARALIGIALCSRWGGNIPES
EEKYSQELEQVVLREGDKAEALRIVWWTKYIGTIMYVICGVHPGGNIRDNVFDFHVSK
RSEVETSLKELIIDDANTTKVKEESTRKNRGYEVVVRISKDDLKTSASVRSRIITLQK
KVRKLSRGSVERVKIGVQFYEE"
gene complement(<27921..>31636)
/gene="HFM1"
/locus_tag="YGL251C"
/gene_synonym="MER3"
/db_xref="GeneID:852641"
mRNA complement(join(<27921..31426,31579..>31636))
/gene="HFM1"
/locus_tag="YGL251C"
/gene_synonym="MER3"
/product="DNA helicase"
/transcript_id="NM_001181117.1"
/db_xref="GeneID:852641"
CDS complement(join(27921..31426,31579..31636))
/gene="HFM1"
/locus_tag="YGL251C"
/gene_synonym="MER3"
/EC_number="5.6.2.4"
/experiment="EXISTENCE:direct assay:GO:0003678 DNA
helicase activity [PMID:11971962]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11971962]"
/experiment="EXISTENCE:direct assay:GO:0006260 DNA
replication [PMID:12039965]"
/experiment="EXISTENCE:direct assay:GO:0051321 meiotic
cell cycle [PMID:11971962]"
/experiment="EXISTENCE:mutant phenotype:GO:0007129
homologous chromosome pairing at meiosis [PMID:15805472]"
/experiment="EXISTENCE:mutant phenotype:GO:0007131
reciprocal meiotic recombination [PMID:15066280]"
/note="Meiosis specific DNA helicase; involved in the
conversion of double-stranded breaks to later
recombination intermediates and in crossover control;
catalyzes the unwinding of Holliday junctions; has ssDNA
and dsDNA stimulated ATPase activity; ZMM family member"
/codon_start=1
/product="DNA helicase"
/protein_id="NP_011263.2"
/db_xref="GeneID:852641"
/db_xref="SGD:S000003220"
/translation="MKTKFDRLGTGKRSRPSPNNIDFNDQSATFKRNKKNSRQPSFKV
GLSYNSLLDDCDDENETEEIFEGRGLQFFDKDDNFSITADDTQVTSKLFDHDLEQTPD
EEAKKPKKVTIRKSAKKCLSTTILPDSFRGVFKFTEFNKMQSEAFPSIYESNENCIIS
SPTGSGKTVLFELAILRLIKETNSDTNNTKIIYIAPTKSLCYEMYKNWFPSFVNLSVG
MLTSDTSFLETEKAKKCNIIITTPEKWDLLTRRWSDYSRLFELVKLVLVDEIHTIKEK
RGASLEVILTRMNTMCQNIRFVALSATVPNIEDLALWLKTNNELPANILSFDESYRQV
QLTKFVYGYSFNCKNDFQKDAIYNSKLIEIIEKHADNRPVLIFCPTRASTISTAKFLL
NNHIFSKSKKRCNHNPSDKILNECMQQGIAFHHAGISLEDRTAVEKEFLAGSINILCS
TSTLAVGVNLPAYLVIIKGTKSWNSSEIQEYSDLDVLQMIGRAGRPQFETHGCAVIMT
DSKMKQTYENLIHGTDVLESSLHLNLIEHLAAETSLETVYSIETAVNWLRNTFFYVRF
GKNPAAYQEVNRYVSFHSVEDSQINQFCQYLLDTLVKVKIIDISNGEYKSTAYGNAMT
RHYISFESMKQFINAKKFLSLQGILNLLATSEEFSVMRVRHNEKKLFKEINLSPLLKY
PFLTEKKQSQIIDRVSQKVSLLIQYELGGLEFPSYEGASKLHQTLVQDKFLVFRHCFR
LLKCMVDTFIEKSDGTSLKNTLFLLRSLNGHCWENTPMVLRQLKTIGLVSVRRLIRHG
ITNLEEMGHLSDTQIEYYLNLKIGNGIKIKNDISLLPCLNIRTKLENCKIENEELWLT
FKVEISATFKSSIWHGQHLSLDIETEKSSGELIDFRRLQVNKLQSPRGFRISAKISPK
LEKIEFSIHCQEIAGLGKTIVYSTDHLASQFSAKTPNIRKDLNSLEKCLFYESSSDGE
VGKTSRVSHKDGLEESLSSDDSILDYLNERKKSSKAVESAAVIHPEAHSSSHFSNGRQ
VRSNGNYECFHSCKDKTQCRHLCCKEGIPVKYIKEKGPSSIKPVSKPDQIRQPLLAKN
INTTPHLEKRLNSKPKQWQEENTDIATVHTLPSKIYNLSQQMSSMEAGEQVLKSGPEN
CPEIIPIDLESSDSYSSNTAASSISDPNGDLDFLGSDIEFE"
gene <31910..>32635
/gene="RMR1"
/locus_tag="YGL250W"
/db_xref="GeneID:852642"
mRNA <31910..>32635
/gene="RMR1"
/locus_tag="YGL250W"
/product="Rmr1p"
/transcript_id="NM_001181116.1"
/db_xref="GeneID:852642"
CDS 31910..32635
/gene="RMR1"
/locus_tag="YGL250W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0006311 meiotic
gene conversion [PMID:18069899]"
/experiment="EXISTENCE:mutant phenotype:GO:0007131
reciprocal meiotic recombination [PMID:18069899]"
/note="Protein required for meiotic recombination and gene
conversion; null mutant displays reduced PIS1 expression
and growth defects on non-fermentable carbon sources and
minimal media; GFP-fusion protein localizes to both
cytoplasm and nucleus"
/codon_start=1
/product="Rmr1p"
/protein_id="NP_011264.2"
/db_xref="GeneID:852642"
/db_xref="SGD:S000003219"
/translation="MSEEEAHKTVEVDDVGVQLDEGDEEDLLEYDDELVEEQPSDARI
RNVAETLMKSELPKVTVEYKDTTFLLFTSDDKNESNNPIICENAALYQRPMGEFMESI
RKFMGNRFGRLAFATKELVLQLKSLDLTLFEDNVYNNHISFSDVYTIFKILKERSESN
FETDIPTHLAIELSTRPRFVSRYNALVELTESSATLKNIKPFSNDETHPLIVDDNDQY
THQNTSEVIVMDIDDDVGEDSED"
gene <33098..>35212
/gene="ZIP2"
/locus_tag="YGL249W"
/db_xref="GeneID:852643"
mRNA <33098..>35212
/gene="ZIP2"
/locus_tag="YGL249W"
/product="Zip2p"
/transcript_id="NM_001181115.1"
/db_xref="GeneID:852643"
CDS 33098..35212
/gene="ZIP2"
/locus_tag="YGL249W"
/experiment="EXISTENCE:direct assay:GO:0000217 DNA
secondary structure binding [PMID:30566683]"
/experiment="EXISTENCE:direct assay:GO:0000795
synaptonemal complex [PMID:9590170]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:22842922]"
/experiment="EXISTENCE:mutant phenotype:GO:0007129
homologous chromosome pairing at meiosis [PMID:15805472]"
/experiment="EXISTENCE:mutant phenotype:GO:0007130
synaptonemal complex assembly [PMID:9590170]"
/experiment="EXISTENCE:mutant phenotype:GO:0007131
reciprocal meiotic recombination [PMID:15066280]"
/experiment="EXISTENCE:mutant phenotype:GO:0033235
positive regulation of protein sumoylation
[PMID:23326245]"
/note="Meiosis-specific protein; involved in normal
synaptonemal complex formation and pairing between
homologous chromosomes during meiosis; relocalizes from
mitochondrion to cytoplasm upon DNA replication stress;
ZMM family member"
/codon_start=1
/product="Zip2p"
/protein_id="NP_011265.1"
/db_xref="GeneID:852643"
/db_xref="SGD:S000003218"
/translation="MIIERWEVKLSKCNQNVGGYSVLSGNLKENIKLGRRAQKYLKEL
RNLQLKPLKIGGYENCGTINGEEYFLEVIHITSGRQKIDVAVGKTWNVTNIENDNKEE
LQYELFKEKLKVGKQDMLFFSWMKSLSVQLNAPLHQKMTEHGLADDNTRLEWFNIPLL
RRSQYRKKVPYPSLRQMSSVLEVQCSTLTEEKLNFCVGFSDKPLSEWKPQIFEQTYNR
YRLQRISPEKSFKYKSRCSKYNFKTSSQSWVVKVPEHDQQLNTFEKRYDELFDAQFNK
LEFFKIRMKKLKKNKPIEKKNYKIWCLEKEDLKDLVWDPLKRICNHSRYAIFEHVTIN
REAYSIKPLRLTFQKLDSGSLDLIDNQKKTFGSIKLAMSMPDVKKTENQSIEESERHD
ETAIETQEFDENDCLSSKADINTSLAPQKRSFIDNELMSMLVTKKKIKKDKDVSDTGI
SSTSYLINSGTYANSHIEIPTSNSVYNGKEDCSFNNYSVKHSILEEDIENKCIAVNEN
KVIENQKVIQSLCKNSHLDLIEQSYFGECDFIINHSTCVYKIQASRFMQLRNNGSLHY
DKAVNDLLTEFQRVIIIVEFSEIIQDVDPDLFWKIKLYLLNSRVDVFFIHETTDFFID
WMKYFIARWAFSYNDEKEKNIANADILLDLGFNILLVRKIFQTYSLEEFFMAIIKEES
KAVKMLTVSQMTRLKKLLTLEW"
gene complement(33109..35013)
/gene="RME3"
/locus_tag="YNCG0001C"
/db_xref="GeneID:23547379"
ncRNA complement(33109..35013)
/ncRNA_class="antisense_RNA"
/gene="RME3"
/locus_tag="YNCG0001C"
/product="RME3"
/experiment="EXISTENCE:direct assay:GO:0010629 negative
regulation of gene expression [PMID:21300780]"
/note="Antisense transcript that represses the
meiosis-specific ZIP2 gene; expressed in haploid cells
under sporulation inducing conditions; regulates ZIP2 in a
cis-dependent manner; RME3 is repressed by the MAT
a1/alpha2 complex in diploid cells"
/transcript_id="NR_132176.1"
/db_xref="GeneID:23547379"
/db_xref="SGD:S000144910"
gene <35653..>36762
/gene="PDE1"
/locus_tag="YGL248W"
/db_xref="GeneID:852644"
mRNA <35653..>36762
/gene="PDE1"
/locus_tag="YGL248W"
/product="3',5'-cyclic-nucleotide phosphodiesterase PDE1"
/transcript_id="NM_001181114.1"
/db_xref="GeneID:852644"
CDS 35653..36762
/gene="PDE1"
/locus_tag="YGL248W"
/EC_number="3.1.4.17"
/experiment="EXISTENCE:genetic interaction:GO:0004115
3',5'-cyclic-AMP phosphodiesterase activity
[PMID:2824992]"
/experiment="EXISTENCE:mutant phenotype:GO:0004115
3',5'-cyclic-AMP phosphodiesterase activity
[PMID:6300049|PMID:2824992]"
/experiment="EXISTENCE:mutant phenotype:GO:0007189
adenylate cyclase-activating G protein-coupled receptor
signaling pathway [PMID:9880329]"
/note="Low-affinity cyclic AMP phosphodiesterase; controls
glucose and intracellular acidification-induced cAMP
signaling, target of the cAMP-protein kinase A (PKA)
pathway; glucose induces transcription and inhibits
translation"
/codon_start=1
/product="3',5'-cyclic-nucleotide phosphodiesterase PDE1"
/protein_id="NP_011266.1"
/db_xref="GeneID:852644"
/db_xref="SGD:S000003217"
/translation="MVVFEITILGANGGPTEYGTQCFILKPARTEDPELIAVDGGAGM
YQLREMLVQGRNENEGDDELVPSFYEHDREPIEFFIDSKLNIQKGLSKSLLQSLKRQG
EHFESANTMKKTYEVFQGITDYYITHPHLDHISGLVVNSPSIYEQENSKKKTIWGLPH
TIDVLQKHVFNDLIWPDLTAERSRKLKLKCLNPKEVQKCTIFPWDVIPFKVHHGIGVK
TGAPVYSTFYIFRDRKSKDCIIVCGDVEQDRRESEESLLEEFWSYVAENIPLVHLKGI
LVECSCPLSSKPEQLYGHLSPIYLINELSNLNTLYNSSKGLSGLNVIVTHVKSTPAKR
DPRLTILEELRFLAEERNLGDLRISIALEGHTLFL"
gene <36933..>37526
/gene="BRR6"
/locus_tag="YGL247W"
/db_xref="GeneID:852645"
mRNA <36933..>37526
/gene="BRR6"
/locus_tag="YGL247W"
/product="Brr6p"
/transcript_id="NM_001181113.1"
/db_xref="GeneID:852645"
CDS 36933..37526
/gene="BRR6"
/locus_tag="YGL247W"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:30133335]"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:26432634|PMID:11483521]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0055088
lipid homeostasis [PMID:20016074|PMID:26432634]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:30133335]"
/experiment="EXISTENCE:mutant phenotype:GO:0006998 nuclear
envelope organization [PMID:20016074]"
/experiment="EXISTENCE:mutant phenotype:GO:0055088 lipid
homeostasis [PMID:20016074|PMID:26432634]"
/note="Essential nuclear envelope integral membrane
protein; interacts with chromatin, helping to maintain
normal chromatin architecture and transcriptional
regulation of certain loci at the nuclear envelope;
interacts and functions with Apq12p and Brl1p in lipid
homeostasis; mutants are defective in nuclear pore complex
biogenesis, nuclear envelope morphology, mRNA nuclear
export, and are sensitive to sterol biosynthesis
inhibitors and membrane fluidizing agents; homologous to
Brl1p"
/codon_start=1
/product="Brr6p"
/protein_id="NP_011267.1"
/db_xref="GeneID:852645"
/db_xref="SGD:S000003216"
/translation="MELRSFSRQPDGILANPRLGREEVLEGEHPQDARLARQSIWLSP
SLIAEYIQLFFNFIIGTIGLSLAIKFILMIRNDVNLKLEHNVREELDKIATCKSRYFE
NQCEPHMRVPALEVRCNEWSKCMNKEIVSGSDYQWAKAWARTLAEVINAFFEAFSIRS
FLFILISIIGIIFVTNTSFGSYRVYLNNKDTKSVRHA"
gene complement(<37617..>38780)
/gene="RAI1"
/locus_tag="YGL246C"
/db_xref="GeneID:852646"
mRNA complement(<37617..>38780)
/gene="RAI1"
/locus_tag="YGL246C"
/product="decapping nuclease"
/transcript_id="NM_001181112.1"
/db_xref="GeneID:852646"
CDS complement(37617..38780)
/gene="RAI1"
/locus_tag="YGL246C"
/experiment="EXISTENCE:direct assay:GO:0000448 cleavage in
ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:26638174]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12612077|PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0030234 enzyme
regulator activity [PMID:10805743]"
/experiment="EXISTENCE:direct assay:GO:0031087
deadenylation-independent decapping of nuclear-transcribed
mRNA [PMID:20802481]"
/experiment="EXISTENCE:direct assay:GO:0034353 mRNA
5'-diphosphatase activity [PMID:20802481]"
/experiment="EXISTENCE:direct assay:GO:1990174
phosphodiesterase decapping endonuclease activity
[PMID:20802481]"
/experiment="EXISTENCE:mutant phenotype:GO:0000463
maturation of LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10805743]"
/experiment="EXISTENCE:mutant phenotype:GO:0000466
maturation of 5.8S rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10805743]"
/experiment="EXISTENCE:mutant phenotype:GO:0000956
nuclear-transcribed mRNA catabolic process
[PMID:20802481]"
/experiment="EXISTENCE:mutant phenotype:GO:0030846
termination of RNA polymerase II transcription,
poly(A)-coupled [PMID:15565157]"
/experiment="EXISTENCE:mutant phenotype:GO:0071035 nuclear
polyadenylation-dependent rRNA catabolic process
[PMID:16131592]"
/experiment="EXISTENCE:mutant phenotype:GO:0110155 NAD-cap
decapping [PMID:33139726]"
/experiment="EXISTENCE:mutant phenotype:GO:1990174
phosphodiesterase decapping endonuclease activity
[PMID:20802481]"
/experiment="EXISTENCE:physical interaction:GO:0005634
nucleus [PMID:10805743]"
/note="Nuclear decapping endonuclease; targets mRNAs with
unmethylated 7-methylguanosine cap structures and
5'-triphosphates; binds to and stabilizes the
exoribonuclease Rat1p; required for pre-rRNA processing;
involved in an early step of decapping non-canonical
nicotinamide adenine dinucleotide (NAD) capped nuclear
mRNAs (NAD-RNAs), part of ncRNA surveillance; relocalizes
to the cytosol in response to hypoxia; homologous to human
DOM3Z"
/codon_start=1
/product="decapping nuclease"
/protein_id="NP_011268.1"
/db_xref="GeneID:852646"
/db_xref="SGD:S000003215"
/translation="MGVSANLFVKQRGSTTALKQPKEIGFYSRTKDEEYLISDDTNLN
YYYLPDAELDRKLDLSSGFQKFKDYYKDFEDRCSLRGLLETIESSERHKGKKINADII
TFRGIARKLISCAFDSPSFNTVDLRIVSFNGQLFIKEVPEAVNAAKASSATEAGRNIN
QDLNVFTGYKFETLATLSNPLQYTPREVIEKRTKRIVSHGDEYISVVRTGVGNCKLIL
GAEVDCIFDFKENGRDNLKHYAELKCTQQVANISDTHKFERKLFRTWLQCFLVGIPRI
IYGFKDDHYVLKTVEEFSTEEVPVLLKNNNPQVGSACLEAIKWYGLLTEWLLKMIPRD
EDPHSQIRAFKLVFENNHLRLSEIEESDEEYSGLIDGEHILSNGFKEWRKSLK"
gene <39023..>41149
/gene="GUS1"
/locus_tag="YGL245W"
/gene_synonym="GSN1"
/db_xref="GeneID:852606"
mRNA <39023..>41149
/gene="GUS1"
/locus_tag="YGL245W"
/gene_synonym="GSN1"
/product="glutamate--tRNA ligase GUS1"
/transcript_id="NM_001181111.1"
/db_xref="GeneID:852606"
CDS 39023..41149
/gene="GUS1"
/locus_tag="YGL245W"
/gene_synonym="GSN1"
/EC_number="6.1.1.17"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0004818
glutamate-tRNA ligase activity [PMID:11069915]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:19417106]"
/experiment="EXISTENCE:direct assay:GO:0006424
glutamyl-tRNA aminoacylation [PMID:11069915]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26359986|PMID:26777405]"
/experiment="EXISTENCE:direct assay:GO:0017102 methionyl
glutamyl tRNA synthetase complex [PMID:11069915]"
/experiment="EXISTENCE:direct assay:GO:1990825
sequence-specific mRNA binding [PMID:34039240]"
/note="Glutamyl-tRNA synthetase (GluRS); forms a complex
with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex
formation increases the catalytic efficiency of both tRNA
synthetases and ensures their correct localization to the
cytoplasm; protein abundance increases in response to DNA
replication stress"
/codon_start=1
/product="glutamate--tRNA ligase GUS1"
/protein_id="NP_011269.2"
/db_xref="GeneID:852606"
/db_xref="SGD:S000003214"
/translation="MPSTLTINGKAPIVAYAELIAARIVNALAPNSIAIKLVDDKKAP
AAKLDDATEDVFNKITSKFAAIFDNGDKEQVAKWVNLAQKELVIKNFAKLSQSLETLD
SQLNLRTFILGGLKYSAADVACWGALRSNGMCGSIIKNKVDVNVSRWYTLLEMDPIFG
EAHDFLSKSLLELKKSANVGKKKETHKANFEIDLPDAKMGEVVTRFPPEPSGYLHIGH
AKAALLNQYFAQAYKGKLIIRFDDTNPSKEKEEFQDSILEDLDLLGIKGDRITYSSDY
FQEMYDYCVQMIKDGKAYCDDTPTEKMREERMDGVASARRDRSVEENLRIFTEEMKNG
TEEGLKNCVRAKIDYKALNKTLRDPVIYRCNLTPHHRTGSTWKIYPTYDFCVPIVDAI
EGVTHALRTIEYRDRNAQYDWMLQALRLRKVHIWDFARINFVRTLLSKRKLQWMVDKD
LVGNWDDPRFPTVRGVRRRGMTVEGLRNFVLSQGPSRNVINLEWNLIWAFNKKVIDPI
APRHTAIVNPVKIHLEGSEAPQEPKIEMKPKHKKNPAVGEKKVIYYKDIVVDKDDADV
INVDEEVTLMDWGNVIITKKNDDGSMVAKLNLEGDFKKTKHKLTWLADTKDVVPVDLV
DFDHLITKDRLEEDESFEDFLTPQTEFHTDAIADLNVKDMKIGDIIQFERKGYYRLDA
LPKDGKPYVFFTIPDGKSVNKYGAKK"
gene <41498..>43174
/gene="RTF1"
/locus_tag="YGL244W"
/gene_synonym="CSL3"
/db_xref="GeneID:852607"
mRNA <41498..>43174
/gene="RTF1"
/locus_tag="YGL244W"
/gene_synonym="CSL3"
/product="RNA polymerase-associated protein"
/transcript_id="NM_001181110.1"
/db_xref="GeneID:852607"
CDS 41498..43174
/gene="RTF1"
/locus_tag="YGL244W"
/gene_synonym="CSL3"
/experiment="EXISTENCE:direct assay:GO:0000791 euchromatin
[PMID:12682017]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:20732871]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:15643076]"
/experiment="EXISTENCE:direct assay:GO:1990269 RNA
polymerase II C-terminal domain phosphoserine binding
[PMID:22796944]"
/experiment="EXISTENCE:direct assay:GO:2001209 positive
regulation of transcription elongation by RNA polymerase I
[PMID:20299458]"
/experiment="EXISTENCE:genetic interaction:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:11884586]"
/experiment="EXISTENCE:genetic interaction:GO:0006368
transcription elongation by RNA polymerase II
[PMID:11927560]"
/experiment="EXISTENCE:genetic interaction:GO:0090262
regulation of transcription-coupled nucleotide-excision
repair [PMID:21737840]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:15180994]"
/experiment="EXISTENCE:mutant phenotype:GO:0001015 snoRNA
transcription by RNA polymerase II [PMID:16246725]"
/experiment="EXISTENCE:mutant phenotype:GO:0006353
DNA-templated transcription termination [PMID:23109428]"
/experiment="EXISTENCE:mutant phenotype:GO:0006368
transcription elongation by RNA polymerase II
[PMID:11927560]"
/experiment="EXISTENCE:mutant phenotype:GO:0009302
sno(s)RNA transcription [PMID:23109428]"
/experiment="EXISTENCE:mutant phenotype:GO:0031124 mRNA
3'-end processing [PMID:15149594]"
/experiment="EXISTENCE:mutant phenotype:GO:0031126
sno(s)RNA 3'-end processing [PMID:16246725]"
/experiment="EXISTENCE:mutant phenotype:GO:0032968
positive regulation of transcription elongation by RNA
polymerase II [PMID:14710186]"
/experiment="EXISTENCE:mutant phenotype:GO:0042138 meiotic
DNA double-strand break formation [PMID:32290544]"
/experiment="EXISTENCE:mutant phenotype:GO:0070911 global
genome nucleotide-excision repair [PMID:21737840]"
/experiment="EXISTENCE:physical interaction:GO:0016593
Cdc73/Paf1 complex [PMID:11884586|PMID:11927560]"
/note="Subunit of RNAPII-associated chromatin remodeling
Paf1 complex; regulates gene expression by directing
cotranscriptional histone modification, influences
transcription and chromatin structure through several
independent functional domains; directly or indirectly
regulates DNA-binding properties of Spt15p and relative
activities of different TATA elements; involved in
transcription elongation as demonstrated by the
G-less-based run-on (GLRO) assay"
/codon_start=1
/product="RNA polymerase-associated protein"
/protein_id="NP_011270.1"
/db_xref="GeneID:852607"
/db_xref="SGD:S000003213"
/translation="MSDLDEDLLALAGADESEEEDQVLTTTSAKRAKNNDQSLSKKRR
IEVGSVEDDDEEDDYNPYSVGNADYGSEEEEEANPFPLEGKYKDESDREHLESLPEME
RETLLFERSQIMQKYQERKLFRARGRDMKEQQQRAKNDEDSRKTRASTRSTHATGHSD
IKASKLSQLKKQRARKNRHYSDNEDEDDEEDYREEDYKDDEGSEYGDDEEYNPFDRRD
TYDKREEVEWAEEEDEQDREPEISDFNKLRIGRSFVAKFCFYPGFEDAVKGCYGRVNV
GTDKRTGKTSYRMVRIERVFLQKPYNMGKFYTNQYFGVTQGKDRKVFQMNYFSDGLFA
EDEYQRYLRALDNSQMIKPSLHSLSNKTKEVMDFVNTPLTDKTTDEVVRHRMQFNKKL
SGTNAVLEKTVLREKLQYAKETNNEKDIAKYSAQLRNFEKRMSVYEKHHENDQSDIKK
LGELTSKNRKLNMSNIRNAEHVKKEDSNNFDSKSDPFSRLKTRTKVYYQEIQKEENAK
AKEIAQQEKLQEDKDAKDKREKELLVAQFRRLGGLERMVGELDIKFDLKF"
gene <43307..>44509
/gene="TAD1"
/locus_tag="YGL243W"
/db_xref="GeneID:852608"
mRNA <43307..>44509
/gene="TAD1"
/locus_tag="YGL243W"
/product="tRNA-specific adenosine deaminase"
/transcript_id="NM_001181109.1"
/db_xref="GeneID:852608"
CDS 43307..44509
/gene="TAD1"
/locus_tag="YGL243W"
/EC_number="3.5.4.34"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0006400 tRNA
modification [PMID:9707437]"
/experiment="EXISTENCE:direct assay:GO:0043829
tRNA-specific adenosine-37 deaminase activity
[PMID:9707437]"
/note="tRNA-specific adenosine deaminase; deaminates
adenosine-37 to inosine in tRNA-Ala"
/codon_start=1
/product="tRNA-specific adenosine deaminase"
/protein_id="NP_011271.1"
/db_xref="GeneID:852608"
/db_xref="SGD:S000003212"
/translation="MVSCQGTRPCIVNLLTMPSEDKLGEEISTRVINEYSKLKSACRP
IIRPSGIREWTILAGVAAINRDGGANKIEILSIATGVKALPDSELQRSEGKILHDCHA
EILALRGANTVLLNRIQNYNPSSGDKFIQHNDEIPARFNLKENWELALYISRLPCGDA
SMSFLNDNCKNDDFIKIEDSDEFQYVDRSVKTILRGRLNFNRRNVVRTKPGRYDSNIT
LSKSCSDKLLMKQRSSVLNCLNYELFEKPVFLKYIVIPNLEDETKHHLEQSFHTRLPN
LDNEIKFLNCLKPFYDDKLDEEDVPGLMCSVKLFMDDFSTEEAILNGVRNGFYTKSSK
PLRKHCQSQVSRFAQWELFKKIRPEYEGISYLEFKSRQKKRSQLIIAIKNILSPDGWI
PTRTDDVK"
gene complement(<44652..>45197)
/gene="ANK1"
/locus_tag="YGL242C"
/db_xref="GeneID:852609"
mRNA complement(<44652..>45197)
/gene="ANK1"
/locus_tag="YGL242C"
/product="ankyrin repeat-containing protein ANK1"
/transcript_id="NM_001181108.1"
/db_xref="GeneID:852609"
CDS complement(44652..45197)
/gene="ANK1"
/locus_tag="YGL242C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:37531259]"
/note="Cytoplasmic ankyrin repeat-containing protein;
co-purifies with Myo3p and Myo5p by large-scale affinity
capture; localization of the long-tailed type I myosins is
altered in the null mutant; N-terminally propionylated in
vivo; contains three ankyrin repeats and an acidic tail;
orthologous to human OSTF1"
/codon_start=1
/product="ankyrin repeat-containing protein ANK1"
/protein_id="NP_011272.1"
/db_xref="GeneID:852609"
/db_xref="SGD:S000003211"
/translation="MNTEGASLSEQLLDAARRNNLDLLETVFDSLDNDPEKIAKLINE
SKEPLGNTALHLCCKYGSWEVLDKILDQDGEIEIDPQNDVDGDTPLHVTVRYSQEEPE
HGTFIARNLIEVGADPRVRNYNNQKPVDLVHGDELDELIDLLQGAELAIDSTNGSGDN
NEDGEMIDDGPSDDDEEDDKK"
gene <45445..>48459
/gene="KAP114"
/locus_tag="YGL241W"
/db_xref="GeneID:852610"
mRNA <45445..>48459
/gene="KAP114"
/locus_tag="YGL241W"
/product="karyopherin KAP114"
/transcript_id="NM_001181107.1"
/db_xref="GeneID:852610"
CDS 45445..48459
/gene="KAP114"
/locus_tag="YGL241W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922|PMID:10385521]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:10385521|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0006606 protein
import into nucleus [PMID:15888545]"
/experiment="EXISTENCE:mutant phenotype:GO:0006606 protein
import into nucleus [PMID:10385521|PMID:15888545]"
/experiment="EXISTENCE:mutant phenotype:GO:0006607
NLS-bearing protein import into nucleus [PMID:12456659]"
/experiment="EXISTENCE:mutant phenotype:GO:0061608 nuclear
import signal receptor activity [PMID:10385521]"
/experiment="EXISTENCE:physical interaction:GO:0061608
nuclear import signal receptor activity [PMID:10385521]"
/note="Karyopherin, responsible for nuclear import of
specific proteins; cargoes include Spt15p, Sua7p, histones
H2A and H2B, and Nap1p; amino terminus shows similarity to
those of other importins, particularly Cse1p; localization
is primarily nuclear; function is regulated by
sumoylation; protein abundance increases in response to
DNA replication stress"
/codon_start=1
/product="karyopherin KAP114"
/protein_id="NP_011273.1"
/db_xref="GeneID:852610"
/db_xref="SGD:S000003210"
/translation="MDINELIIGAQSADKHTREVAETQLLQWCDSDASQVFKALANVA
LQHEASLESRQFALLSLRKLITMYWSPGFESYRSTSNVEIDVKDFIREVLLKLCLNDN
ENTKIKNGASYCIVQISAVDFPDQWPQLLTVIYDAISHQHSLNAMSLLNEIYDDVVSE
EMFFEGGIGLATMEIVFKVLNTETSTLIAKIAALKLLKACLLQMSSHNEYDEASRKSF
VSQCLATSLQILGQLLTLNFGNVDVISQLKFKSIIYENLVFIKNDFSRKHFSSELQKQ
FKIMAIQDLENVTHINANVETTESEPLLETVHDCSIYIVEFLTSVCTLQFSVEEMNKI
ITSLTILCQLSSETREIWTSDFNTFVSKETGLAASYNVRDQANEFFTSLPNPQLSLIF
KVVSNDIEHSTCNYSTLESLLYLLQCILLNDDEITGENIDQSLQILIKTLENILVSQE
IPELILARAILTIPRVLDKFIDALPDIKPLTSAFLAKSLNLALKSDKELIKSATLIAF
TYYCYFAELDSVLGPEVCSETQEKVIRIINQVSSDAEEDTNGALMEVLSQVISYNPKE
PHSRKEILQAEFHLVFTISSEDPANVQVVVQSQECLEKLLDNINMDNYKNYIELCLPS
FINVLDSNNANNYRYSPLLSLVLEFITVFLKKKPNDGFLPDEINQYLFEPLAKVLAFS
TEDETLQLATEAFSYLIFNTDTRAMEPRLMDIMKVLERLLSLEVSDSAAMNVGPLVVA
IFTRFSKEIQPLIGRILEAVVVRLIKTQNISTEQNLLSVLCFLTCNDPKQTVDFLSSF
QIDNTDALTLVMRKWIEAFEVIRGEKRIKENIVALSNLFFLNDKRLQKVVVNGNLIPY
EGDLIITRSMAKKMPDRYVQVPLYTKIIKLFVSELSFQSKQPNPEQLITSDIKQEVVN
ANKDDDNDDWEDVDDVLDYDKLKEYIDDDVDEEADDDSDDITGLMDVKESVVQLLVRF
FKEVASKDVSGFHCIYETLSDSERKVLSEALL"
gene <48613..>49365
/gene="DOC1"
/locus_tag="YGL240W"
/gene_synonym="APC10"
/db_xref="GeneID:852611"
mRNA <48613..>49365
/gene="DOC1"
/locus_tag="YGL240W"
/gene_synonym="APC10"
/product="anaphase promoting complex subunit DOC1"
/transcript_id="NM_001181106.1"
/db_xref="GeneID:852611"
CDS 48613..49365
/gene="DOC1"
/locus_tag="YGL240W"
/gene_synonym="APC10"
/experiment="EXISTENCE:direct assay:GO:0005680
anaphase-promoting complex [PMID:9469814]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0030234 enzyme
regulator activity [PMID:12402045]"
/experiment="EXISTENCE:direct assay:GO:0031145
anaphase-promoting complex-dependent catabolic process
[PMID:12402045]"
/experiment="EXISTENCE:direct assay:GO:0061630 ubiquitin
protein ligase activity [PMID:12402045]"
/experiment="EXISTENCE:genetic interaction:GO:0006325
chromatin organization [PMID:12399376]"
/experiment="EXISTENCE:mutant phenotype:GO:0031145
anaphase-promoting complex-dependent catabolic process
[PMID:9348530|PMID:12402045]"
/note="Processivity factor; required for ubiquitination
activity of anaphase promoting complex (APC), mediates the
activity of APC by contributing to substrate recognition;
involved in cyclin proteolysis; contains conserved DOC1
homology domain"
/codon_start=1
/product="anaphase promoting complex subunit DOC1"
/protein_id="NP_011274.2"
/db_xref="GeneID:852611"
/db_xref="SGD:S000003209"
/translation="MDPIGINKVLDHLAPSELIKPVKSCHNKPSVLVLDDRIVDAATK
DLYVNGFQEEIQYQNPTPENLQHMFHQGIEILDSARMINVTHLALWKPSSFKLGNPVD
FALDDNYDTFWQSDGGQPHQLDIMFSKRMDICVMAIFFSMIADESYAPSLVKVYAGHS
PSDARFYKMLEVRNVNGWVALRFLDNREDDQLLKCQFIRLLFPVNHENGKDTHLRGIR
LYVPSNEPHQDTHEWAQTLPETNNVFQDAILR"
gene <49552..>52434
/gene="CSE1"
/locus_tag="YGL238W"
/gene_synonym="KAP109"
/db_xref="GeneID:852612"
mRNA <49552..>52434
/gene="CSE1"
/locus_tag="YGL238W"
/gene_synonym="KAP109"
/product="importin-alpha export receptor"
/transcript_id="NM_001181104.1"
/db_xref="GeneID:852612"
CDS 49552..52434
/gene="CSE1"
/locus_tag="YGL238W"
/gene_synonym="KAP109"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:9857050]"
/experiment="EXISTENCE:genetic interaction:GO:0005049
nuclear export signal receptor activity [PMID:9857050]"
/experiment="EXISTENCE:genetic interaction:GO:0006611
protein export from nucleus [PMID:9857050]"
/experiment="EXISTENCE:mutant phenotype:GO:0061015 snRNA
import into nucleus [PMID:31189105]"
/experiment="EXISTENCE:physical interaction:GO:0005049
nuclear export signal receptor activity [PMID:9857050]"
/experiment="EXISTENCE:physical interaction:GO:0006611
protein export from nucleus [PMID:9857050]"
/note="Nuclear envelope protein that acts as a recycling
factor; mediates the nuclear export of Srp1p (importin
alpha) back to the cytoplasm after its import substrates
have been released into the nucleoplasm, thereby allowing
the participation of Srp1p in multiple rounds of nuclear
import; required for accurate chromosome segregation;
homolog of metazoan CAS and human CSE1L, overexpression of
which is implicated in cancer progression"
/codon_start=1
/product="importin-alpha export receptor"
/protein_id="NP_011276.1"
/db_xref="GeneID:852612"
/db_xref="SGD:S000003207"
/translation="MSDLETVAKFLAESVIASTAKTSERNLRQLETQDGFGLTLLHVI
ASTNLPLSTRLAGALFFKNFIKRKWVDENGNHLLPANNVELIKKEIVPLMISLPNNLQ
VQIGEAISSIADSDFPDRWPTLLSDLASRLSNDDMVTNKGVLTVAHSIFKRWRPLFRS
DELFLEIKLVLDVFTAPFLNLLKTVDEQITANENNKASLNILFDVLLVLIKLYYDFNC
QDIPEFFEDNIQVGMGIFHKYLSYSNPLLEDPDETEHASVLIKVKSSIQELVQLYTTR
YEDVFGPMINEFIQITWNLLTSISNQPKYDILVSKSLSFLTAVTRIPKYFEIFNNESA
MNNITEQIILPNVTLREEDVELFEDDPIEYIRRDLEGSDTDTRRRACTDFLKELKEKN
EVLVTNIFLAHMKGFVDQYMSDPSKNWKFKDLYIYLFTALAINGNITNAGVSSTNNLL
NVVDFFTKEIAPDLTSNNIPHIILRVDAIKYIYTFRNQLTKAQLIELMPILATFLQTD
EYVVYTYAAITIEKILTIRESNTSPAFIFHKEDISNSTEILLKNLIALILKHGSSPEK
LAENEFLMRSIFRVLQTSEDSIQPLFPQLLAQFIEIVTIMAKNPSNPRFTHYTFESIG
AILNYTQRQNLPLLVDSMMPTFLTVFSEDIQEFIPYVFQIIAFVVEQSATIPESIKPL
AQPLLAPNVWELKGNIPAVTRLLKSFIKTDSSIFPDLVPVLGIFQRLIASKAYEVHGF
DLLEHIMLLIDMNRLRPYIKQIAVLLLQRLQNSKTERYVKKLTVFFGLISNKLGSDFL
IHFIDEVQDGLFQQIWGNFIITTLPTIGNLLDRKIALIGVLNMVINGQFFQSKYPTLI
SSTMNSIIETASSQSIANLKNDYVDLDNLEEISTFGSHFSKLVSISEKPFDPLPEIDV
NNGVRLYVAEALNKYNAISGNTFLNTILPQLTQENQVKLNQLLVGN"
gene complement(<52731..>53528)
/gene="HAP2"
/locus_tag="YGL237C"
/db_xref="GeneID:852614"
mRNA complement(<52731..>53528)
/gene="HAP2"
/locus_tag="YGL237C"
/product="transcription activator HAP2"
/transcript_id="NM_001181103.1"
/db_xref="GeneID:852614"
CDS complement(52731..53528)
/gene="HAP2"
/locus_tag="YGL237C"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:7828851]"
/experiment="EXISTENCE:direct assay:GO:0001228 DNA-binding
transcription activator activity, RNA polymerase
II-specific [PMID:2832951]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0016602
CCAAT-binding factor complex [PMID:2832951]"
/experiment="EXISTENCE:direct assay:GO:0045944 positive
regulation of transcription by RNA polymerase II
[PMID:2832951]"
/experiment="EXISTENCE:mutant phenotype:GO:0043457
regulation of cellular respiration [PMID:2182199]"
/experiment="EXISTENCE:physical interaction:GO:0016602
CCAAT-binding factor complex [PMID:2832951]"
/note="Subunit of the Hap2p/3p/4p/5p CCAAT-binding
complex; complex is heme-activated and glucose-repressed;
complex is a transcriptional activator and global
regulator of respiratory gene expression; contains
sequences sufficient for both complex assembly and DNA
binding; respiratory defect of the null mutant is
functionally complemented by human NFYA"
/codon_start=1
/product="transcription activator HAP2"
/protein_id="NP_011277.1"
/db_xref="GeneID:852614"
/db_xref="SGD:S000003206"
/translation="MSADETDAKFHPLETDLQSDTAAATSTAAASRSPSLQEKPIEMP
LDMGKAPSPRGEDQRVTNEEDLFLFNRLRASQNRVMDSLEPQQQSQYTSSSVSTMEPS
ADFTSFSAVTTLPPPPHQQQQQQQQQQQQQQLVVQAQYTQNQPNLQSDVLGTAIAEQP
FYVNAKQYYRILKRRYARAKLEEKLRISRERKPYLHESRHKHAMRRPRGEGGRFLTAA
EIKAMKSKKSGASDDPDDSHEDKKITTKIIQEQPHATSTAAAADKKT"
gene complement(<53787..>55796)
/gene="MTO1"
/locus_tag="YGL236C"
/gene_synonym="IPS1"
/db_xref="GeneID:852616"
mRNA complement(<53787..>55796)
/gene="MTO1"
/locus_tag="YGL236C"
/gene_synonym="IPS1"
/product="tRNA modification protein MTO1"
/transcript_id="NM_001181102.1"
/db_xref="GeneID:852616"
CDS complement(53787..55796)
/gene="MTO1"
/locus_tag="YGL236C"
/gene_synonym="IPS1"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:9774408]"
/experiment="EXISTENCE:mutant phenotype:GO:0070899
mitochondrial tRNA wobble uridine modification
[PMID:15509579]"
/note="Mitochondrial protein; forms heterodimer complex
with Mss1p that performs 5-carboxymethylaminomethyl
modification of the wobble uridine base in mitochondrial
tRNAs; required for respiration in paromomycin-resistant
15S rRNA mutants; human homolog MTO1 can complement yeast
null mutant"
/codon_start=1
/product="tRNA modification protein MTO1"
/protein_id="NP_011278.2"
/db_xref="GeneID:852616"
/db_xref="SGD:S000003205"
/translation="MLRVTTLASSCTSFPLQVLRRRLTISSLTSFQPTTKTQVVVIGA
GHAGCEAAAASSRTGAHTTLITPSLTDIGKCSCNPSIGGVGKGILVKEIDALDGLMGK
VTDLAGVQFKMLNRSKGPAVWGPRAQIDRELYKKYMQRELSDKKAHPNLSLLQNKVAD
LILYDPGCGHKVIKGVVLDDGTQVGADQVIITTGTFLSAEIHIGDKRIAAGRIGEQPT
YGISNTLQNEVGFQLGRLKTGTPARLAKESIDFSALEVQKGDALPVPMSFLNETVSVE
PTKQLDCFGTHTTPQMHDFLRNNLHQSIHIQDTTIKGPRYCPSIEAKILRFPDRSSHK
IWLEPEGFNSDVIYPNGISNSMPEDVQLQMMRLIPGMANVEILQPAYGVEYDYVDPRQ
LKPSLETKLVDGLFLAGQINGTTGYEEAAAQGIIAGINAGLLSRQEREQLVLKRSEAY
IGVLIDDLINNGVIEPYRMFTSRSEFRISVRADNADFRLTPIGAQLGIISPVRLSQYS
RDKHLYDETIRALQNFKLSSQKWSSLLQANIAPQAENRSAWEIFRFKDMDLHKLYECI
PDLPINLLDIPMHVVTKINIQGKYEPYIVKQNQFVKAFQADENMLLPQDYDYRQLPTL
STECKLLLNRVQPLTIGQARRIQGITAAALFELYRVARKPSQPVM"
gene <55279..>55815
/locus_tag="YGL235W"
/db_xref="GeneID:852615"
mRNA <55279..>55815
/locus_tag="YGL235W"
/product="uncharacterized protein"
/transcript_id="NM_001181101.1"
/db_xref="GeneID:852615"
CDS 55279..55815
/locus_tag="YGL235W"
/note="hypothetical protein; potential Cdc28p substrate;
null mutant displays increased resistance to antifungal
agents gliotoxin, cycloheximide and H2O2"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011279.1"
/db_xref="GeneID:852615"
/db_xref="SGD:S000003204"
/translation="MTLWPHPGSYKIKSATLFCSRDKLGCAFLSESSLCMYFLYNSLS
IWALGPHTAGPLLLFSILNCTPARSVTLPISPSRASISFTRMPLPTPPIEGLHEHLPI
SVNDGVMRVVCAPVLDDAAAASQPACPAPMTTTCVLVVGWKLVKEDMVNRLLRTCKGN
EVHEDAKVVTRSIVLWGV"
gene <56482..>58890
/gene="ADE57"
/locus_tag="YGL234W"
/db_xref="GeneID:852617"
mRNA <56482..>58890
/gene="ADE57"
/locus_tag="YGL234W"
/product="bifunctional aminoimidazole ribotide
synthase/glycinamide ribotide synthase"
/transcript_id="NM_001181100.1"
/db_xref="GeneID:852617"
CDS 56482..58890
/gene="ADE57"
/locus_tag="YGL234W"
/EC_number="6.3.3.1"
/EC_number="6.3.4.13"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276|PMID:14562095]"
/note="Bifunctional enzyme of 'de novo' purine nucleotide
pathway; contains both phosphoribosylglycinamidine
synthetase/glycinamide ribotide synthetase (EC 6.3.4.13)
['Ade5'] and phosphoribosylaminoimidazole
synthetase/aminoimidazole ribotide synthetase (EC 6.3.3.1)
['Ade7'] activities"
/codon_start=1
/product="bifunctional aminoimidazole ribotide
synthase/glycinamide ribotide synthase"
/protein_id="NP_011280.1"
/db_xref="GeneID:852617"
/db_xref="SGD:S000003203"
/translation="MLNILVLGNGAREHVLVTKLAQSPTVGKIYVAPGNGGTATMDPS
RVINWDITPDVANFARLQSMAVEHKINLVVPGPELPLVNGITSVFHSVGIPVFGPSVK
AAQLEASKAFSKRFMSKHNIPTASYDVFTNPEEAISFLQAHTDKAFVIKADGIAAGKG
VIIPSSIDESVQAIKDIMVTKQFGEEAGKQVVIEQFLEGDEISLLTIVDGYSHFNLPV
AQDHKRIFDGDKGLNTGGMGAYAPAPVATPSLLKTIDSQIVKPTIDGMRRDGMPFVGV
LFTGMILVKDSKTNQLVPEVLEYNVRFGDPETQAVLSLLDDQTDLAQVFLAAAEHRLD
SVNIGIDDTRSAVTVVVAAGGYPESYAKGDKITLDTDKLPPHTQIFQAGTKYDSATDS
LLTNGGRVLSVTSTAQDLRTAVDTVYEAVKCVHFQNSYYRKDIAYRAFQNSESSKVAI
TYADSGVSVDNGNNLVQTIKEMVRSTRRPGADSDIGGFGGLFDLAQAGFRQNEDTLLV
GATDGVGTKLIIAQETGIHNTVGIDLVAMNVNDLVVQGAEPLFFLDYFATGALDIQVA
SDFVSGVANGCIQSGCALVGGETSEMPGMYPPGHYDTNGTAVGAVLRQDILPKINEMA
AGDVLLGLASSGVHSNGFSLVRKIIQHVALPWDAPCPWDESKTLGEGILEPTKIYVKQ
LLPSIRQRLLLGLAHITGGGLVENIPRAIPDHLQARVDMSTWEVPRVFKWFGQAGNVP
HDDILRTFNMGVGMVLIVKRENVKAVCDSLTEEGEIIWELGSLQERPKDAPGCVIENG
TKLY"
gene <59122..>61854
/gene="SEC15"
/locus_tag="YGL233W"
/db_xref="GeneID:852618"
mRNA <59122..>61854
/gene="SEC15"
/locus_tag="YGL233W"
/product="Rab GTPase-binding exocyst subunit SEC15"
/transcript_id="NM_001181099.1"
/db_xref="GeneID:852618"
CDS 59122..61854
/gene="SEC15"
/locus_tag="YGL233W"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:16478783]"
/experiment="EXISTENCE:direct assay:GO:0000145 exocyst
[PMID:8978675|PMID:1512289|PMID:7615633]"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:16478783]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:26928762|PMID:16478783]"
/experiment="EXISTENCE:direct assay:GO:0031267 small
GTPase binding [PMID:10022848]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807]"
/experiment="EXISTENCE:genetic interaction:GO:0006893
Golgi to plasma membrane transport [PMID:7026045]"
/experiment="EXISTENCE:mutant phenotype:GO:0006887
exocytosis [PMID:6996832]"
/experiment="EXISTENCE:mutant phenotype:GO:0006893 Golgi
to plasma membrane transport [PMID:7026045]"
/note="Essential 113 kDa subunit of the exocyst complex;
the exocyst mediates polarized targeting and tethering of
post-Golgi secretory vesicles to active sites of
exocytosis prior to SNARE-mediated fusion; interacts with
and functions as a downstream effector of active,
GTP-bound Sec4p, a Rab family GTPase"
/codon_start=1
/product="Rab GTPase-binding exocyst subunit SEC15"
/protein_id="NP_011281.1"
/db_xref="GeneID:852618"
/db_xref="SGD:S000003202"
/translation="MDQEGQPLLSKDFQQVLLATASGNNSSWTERAVLNNESTDAVKH
EPALGQNDVFDLDPLSFDKWVPFLRRALDKNQLDPVIDELENSIEDNFQGLELQLLQD
SQMNDKLETSIDEIANIQGMVQDTLSSEISKFQIRLSESANELIVKKQMYVNNKKISL
KISEATILITKVVRILELSSKCQELITERKFFKVLQNLDSLEKLYLQEFKNYNFQFLI
EIYNSIPFLQKVTKDECINLIRNSLNLNLGKNLIKVGQEFVAIYENELLPQWLETRSK
MKLTNFKFNSPIEISMRDESFLAKLNLGEFFQLDDFHDSIMIFQNLNELSVLSGEFNK
EYELRKTKLMYPLIWKKNKTAAYQMDSLLRGTGTTPGSTAHDVSTDDPFTQSLSLHFL
QDYFLKILGFLLYDINLNKATEFILVDNNYNSTNEFWDGLMDRLSPYLSYFIDEKLKT
EEDMIKLKDFLCIYVAILENFKLNIEPLYKILVSIFEKFCSVSLRAFDDEFQILLNDD
DFMPLSINDKTLYEKVLKICWMKEGEHLSLPDPTNGEPFAVTLPFSPLYPMTCTLAKK
TYSKITAFLSIFYRHELHTLNNILVKTMDDIFNDIVNKKIRSKLESTSREEIAQILVN
LDYFIIAAKEFSNFMTRENILQNPDMEIRLSSIKYLAESRKLAETKLIELIDSKISDI
LETIEIDWQITEVRQDPDISIIDLAQFLEMMFASTLQNLPYSVQTLLIFREFDSLTRQ
FMGLLLHDTPSTITHESIMNFEVDVNYLESIIPRIFPSTPGTIDSNGYQSPMTPSTPT
FPNANGVDAPTLFENNIKSLEATFMELKQCIELLKTQGKDYNEPEIRLRKYSRIRQED
AALLLSKIQHFVSSVEGANGDDTSVMDSSSIFNSESASVIDSNTSRIAKFFNRR"
gene <62075..>63002
/gene="TAN1"
/locus_tag="YGL232W"
/db_xref="GeneID:852619"
mRNA join(<62075..62131,62190..>63002)
/gene="TAN1"
/locus_tag="YGL232W"
/product="putative tRNA acetyltransferase"
/transcript_id="NM_001181098.1"
/db_xref="GeneID:852619"
CDS join(62075..62131,62190..63002)
/gene="TAN1"
/locus_tag="YGL232W"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:15037780]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0051391 tRNA
acetylation [PMID:38613394]"
/experiment="EXISTENCE:mutant phenotype:GO:0006400 tRNA
modification [PMID:15037780]"
/note="Adaptor in Kre33p-mediated N4-acetylcytidine
modification of tRNAs; tRNA-binding protein required for
the formation of the modified nucleoside
N(4)-acetylcytidine (ac4C) in serine and leucine tRNAs but
not required for the same modification in 18S rRNA;
protein abundance increases in response to DNA replication
stress; contains a conserved THUMP domain, and is
homologous to human THUMPD1"
/codon_start=1
/product="putative tRNA acetyltransferase"
/protein_id="NP_011282.1"
/db_xref="GeneID:852619"
/db_xref="SGD:S000003201"
/translation="MGEKRNRNGKDANSQNRKKFKVSSGFLDPGTSGIYATCSRRHER
QAAQELQLLFEEKFQELYGDIKEGEDESENDEKKDLSIEDQIKKELQELKGEETGKDL
SSGETKKKDPLAFIDLNCECVTFCKTRKPIVPEEFVLSIMKDLADPKNMVKRTRYVQK
LTPITYSCNAKMEQLIKLANLVIGPHFHDPSNVKKNYKFAVEVTRRNFNTIERMDIIN
QVVKLVNKEGSEFNHTVDLKNYDKLILVECFKSNIGMCVVDGDYKTKYRKYNVQQLYE
SKFRKDEDKSVKQ"
gene complement(<63048..>63620)
/gene="EMC4"
/locus_tag="YGL231C"
/db_xref="GeneID:852620"
mRNA complement(<63048..>63620)
/gene="EMC4"
/locus_tag="YGL231C"
/product="chaperone EMC4"
/transcript_id="NM_001181097.1"
/db_xref="GeneID:852620"
CDS complement(63048..63620)
/gene="EMC4"
/locus_tag="YGL231C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0072546 EMC complex
[PMID:19325107]"
/experiment="EXISTENCE:genetic interaction:GO:0034975
protein folding in endoplasmic reticulum [PMID:19325107]"
/note="Member of conserved ER transmembrane complex;
required for efficient folding of proteins in the ER; null
mutant displays induction of the unfolded protein
response; homologous to worm ZK616.6/EMC-4 and fly
CG11137; mutation is functionally complemented by human
EMC4"
/codon_start=1
/product="chaperone EMC4"
/protein_id="NP_011283.1"
/db_xref="GeneID:852620"
/db_xref="SGD:S000003200"
/translation="MSEQEPYEWAKHLLDTKYIEKYNIQNSNTLPSPPGFEGNSSKGN
VTRKQQDATSQTTSLAQKNQITVLQVQKAWQIALQPAKSIPMNIFMSYMSGTSLQIIP
IMTALMLLSGPIKAIFSTRSAFKPVLGNKATQSQVQTAMFMYIVFQGVLMYIGYRKLN
SMGLIPNAKGDWLPWERIAHYNNGLQWFSD"
gene complement(<63772..>64215)
/locus_tag="YGL230C"
/db_xref="GeneID:852621"
mRNA complement(<63772..>64215)
/locus_tag="YGL230C"
/product="uncharacterized protein"
/transcript_id="NM_001181096.1"
/db_xref="GeneID:852621"
CDS complement(63772..64215)
/locus_tag="YGL230C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/note="hypothetical protein; non-essential gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011284.1"
/db_xref="GeneID:852621"
/db_xref="SGD:S000003199"
/translation="MGIITLSGNVLHLLKAYPKKGLEEVSQPEPNTANDSSTEYKGKS
KDDFQMVEKSNTDERYNFTRTKKWFLLMTSEYYKLMENRLLMFCIIACSFICAIQFLF
FIIYWTNIVPRKTQRAITNLNYDYLTAHLKEQCVPYAKILDQCIL"
rep_origin 64360..64464
/note="ARS702; Autonomously Replicating Sequence"
/db_xref="SGD:S000118451"
gene complement(<64503..>66959)
/gene="SAP4"
/locus_tag="YGL229C"
/db_xref="GeneID:852622"
mRNA complement(<64503..>66959)
/gene="SAP4"
/locus_tag="YGL229C"
/product="Sap4p"
/transcript_id="NM_001181095.1"
/db_xref="GeneID:852622"
CDS complement(64503..66959)
/gene="SAP4"
/locus_tag="YGL229C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:mutant phenotype:GO:0000082 G1/S
transition of mitotic cell cycle [PMID:8649382]"
/note="Protein required for function of the Sit4p protein
phosphatase; member of a family of similar proteins that
form complexes with Sit4p, including Sap155p, Sap185p, and
Sap190p; SAP4 has a paralog, SAP155, that arose from the
whole genome duplication"
/codon_start=1
/product="Sap4p"
/protein_id="NP_011285.1"
/db_xref="GeneID:852622"
/db_xref="SGD:S000003198"
/translation="MSLWPFGETLSHSGIDSILEEYYLIFRSLEGNETSSTDDKKNEP
SMESESEFGTESRDRSDLNQSFIDRILLETALLDELNGAANDRLVDFICLGYFYDDRS
QQVRHMDYLVDMLMAYLKDIDRTGYRTPFLLENSFHQTGEYEDQDDEDPMLYVNIISS
IFCSKSAPIVEALVQNTPFLSSLFEVFQFENIEAENCPILAVFLKINETLLFEQTSSY
LEFFKSQPNIVDKFLYHIEVSPLVEFLIKIMLTDQVESPTNIIDFLYHQDLIPKCLNL
LENSKYSPGIQNSSGELLKALISISTNFKLDTLWIGPNRLTRQLASPQYVDQLINIIL
FQRGHAMGVAVSIIIELIRKNNSDYDEVDLLSTTIVDNPPSQRDPVYLGHLLYELTMH
MEDFYALLIKLENDDDDDHDTASKALPSVKHHLLENQLHESFRPLGFERVKITELISE
MLHCSNMGLMNSKRGEKIARTRDKCRDTLDQNSLEKAMKNLNINDNTITSNTLEDKCN
NNDSNDSNDNQKQKKNIKKKFHDNELYSTFDTSDDNIDDDDDMSFEIPYVSETQNLKI
RKNPTIGDLFKIKLHDLGFFPKFLQLFLRYPWNNFWHNIVFDIIQQIFNGRMDFSYNS
FLVYSLFDFKKSTRFIPKPLYGSNQKLPVKDFHIISDFILQGHKDSFEFYEKEKTNLG
YMGQLVLIAEEIAKYSKIYKTDLIAPDIYAFLQDEVWMSYSSDILNETRTMCSIILGG
GQFCAESDENTNQDFLEKADMSKPAHPSTMDENEIVHEEDVKLHDKVAELIDELGQLT
ELDIHDKIKDVIVDHHSDLN"
gene <67598..>69331
/gene="SHE10"
/locus_tag="YGL228W"
/db_xref="GeneID:852623"
mRNA <67598..>69331
/gene="SHE10"
/locus_tag="YGL228W"
/product="She10p"
/transcript_id="NM_001181094.1"
/db_xref="GeneID:852623"
CDS 67598..69331
/gene="SHE10"
/locus_tag="YGL228W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0030476
ascospore wall assembly [PMID:23966878]"
/note="Protein involved in outer spore wall assembly;
likely involved directly in dityrosine layer assembly;
putative GPI-anchored protein; overexpression causes
growth arrest; SWAT-GFP, seamless-GFP and mCherry fusion
proteins localize to the endoplasmic reticulum; SHE10 has
a paralog, OSW7/YFR039C, that arose from the whole genome
duplication; paralogs are redundant for spore wall
dityrosine assembly"
/codon_start=1
/product="She10p"
/protein_id="NP_011286.1"
/db_xref="GeneID:852623"
/db_xref="SGD:S000003197"
/translation="MGKLIKLITTLTVLVSLLQYCCEFNSGSISCERTQTLCHYTNPR
VWNTYFSRNCELYKNKVSPGFDIVARKYDTAVKPVIDDATVKVNKVAIQPAFKVIHSQ
CKKWNCGKYYQLVRSPMVKTRRFFFAKYNAFVKPNLDKFFTAEFRSHLKERILKYKNI
GHYYFTITSRCIKSKYDFIVGNTEEKLMGKFKNKDTHGIHGSVTREPSSEDMVLTVST
MESDEEELTTTSTQTVVETITLDQEEASAVANHAHDDEASTDVEGSTDVNVNEQALLQ
EDFDMWSETILQKTQDVIQLFEKDVSKYINGKLVEEANHFKAKFQSLDDKSKKFFSKI
SLAINDIECVEGIDSETGKKIFFDKSGSTEISQYITRELVREYFNETRSTLDELTNAM
EKDLSEITDEIEKKVNAIREENVEVFEEWGDIIVNEWSKRMAYVDVINAHMGADDDTT
LDEEKAKSSVNWKKFLKGKKQIIESRDKLAHHSADLSRVNAFRQKVQKKILSFTQESG
EFLYILRSKANLQFQERERKERERKEREKAAAEEFQRQQELLRQQEEEDEEDVSYTST
STITTTITMTL"
gene <69671..>72547
/gene="VID30"
/locus_tag="YGL227W"
/gene_synonym="GID1"
/db_xref="GeneID:852624"
mRNA <69671..>72547
/gene="VID30"
/locus_tag="YGL227W"
/gene_synonym="GID1"
/product="glucose-induced degradation complex subunit
VID30"
/transcript_id="NM_001181093.1"
/db_xref="GeneID:852624"
CDS 69671..72547
/gene="VID30"
/locus_tag="YGL227W"
/gene_synonym="GID1"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0034657 GID complex
[PMID:18508925|PMID:16872538]"
/experiment="EXISTENCE:mutant phenotype:GO:0006808
regulation of nitrogen utilization [PMID:11356843]"
/experiment="EXISTENCE:mutant phenotype:GO:0043161
proteasome-mediated ubiquitin-dependent protein catabolic
process [PMID:15358789|PMID:12686616]"
/experiment="EXISTENCE:mutant phenotype:GO:0045721
negative regulation of gluconeogenesis [PMID:12686616]"
/note="Central component of GID Complex, involved in
FBPase degradation; interacts strongly with Gid8p to serve
as a scaffold for other GID Complex subunits; contains
SPRY domain and 3 domains that are also found in Gid8p -
LisH, CTLH, and CRA; required for association of Vid
vesicles and actin patches in vacuole import and
degradation pathway; shifts the balance of nitrogen
metabolism toward glutamate production; localizes to the
nucleus and the cytoplasm"
/codon_start=1
/product="glucose-induced degradation complex subunit
VID30"
/protein_id="NP_011287.1"
/db_xref="GeneID:852624"
/db_xref="SGD:S000003196"
/translation="MSEYMDDVDREFINCLFPSYLLQQPVAYDLWILYLQHRKLFHKL
KNTNLINADENPTGVGMGRTKLTALTRKEIWSKLMNLGVLGTISFEAVNDDYLIQVYK
YFYPDVNDFTLRFGVKDSNKNSVRVMKASSDMRKNAQELLEPVLSEREMALNSNTSLE
NDRNDDDDDDDDDDDDDDDDDDDDDESDLESLEGEVDTDTDDNNEGDGSDNHEEGGEE
GSRGADADVSSAQQRAERVADPWIYQRSRSAINIETESRNLWDTSDKNSGLQYYPPDQ
SPSSSFSSPRVSSGNDKNDNEATNVLSNSGSKKKNSMIPDIYKILGYFLPSRWQAQPN
NSLQLSQDGITHLQPNPDYHSYMTYERSSASSASTRNRLRTSFENSGKVDFAVTWANK
SLPDNKLTIFYYEIKVLSVTSTESAENSNIVIGYKLVENELMEATTKKSVSRSSVAGS
SSSLGGSNNMSSNRVPSTSFTMEGTQRRDYIYEGGVSAMSLNVDGSINKCQKYGFDLN
VFGYCGFDGLITNSTEQSKEYAKPFGRDDVIGCGINFIDGSIFFTKNGIHLGNAFTDL
NDLEFVPYVALRPGNSIKTNFGLNEDFVFDIIGYQDKWKSLAYEHICRGRQMDVSIEE
FDSDESEEDETENGPEENKSTNVNEDLMDIDQEDGAAGNKDTKKLNDEKDNNLKFLLG
EDNRFIDGKLVRPDVNNINNLSVDDGSLPNTLNVMINDYLIHEGLVDVAKGFLKDLQK
DAVNVNGQHSESKDVIRHNERQIMKEERMVKIRQELRYLINKGQISKCINYIDNEIPD
LLKNNLELVFELKLANYLVMIKKSSSKDDDEIENLILKGQELSNEFIYDTKIPQSLRD
RFSGQLSNVSALLAYSNPLVEAPKEISGYLSDEYLQERLFQVSNNTILTFLHKDSECA
LENVISNTRAMLSTLLEYNAFGSTNSSDPRYYKAINFDEDVLNL"
gene complement(<72749..>73158)
/gene="OST5"
/locus_tag="YGL226C-A"
/db_xref="GeneID:852625"
mRNA complement(join(<72749..72988,73138..>73158))
/gene="OST5"
/locus_tag="YGL226C-A"
/product="dolichyl-diphosphooligosaccharide--protein
glycotransferase subunit"
/transcript_id="NM_001181091.1"
/db_xref="GeneID:852625"
CDS complement(join(72749..72988,73138..73158))
/gene="OST5"
/locus_tag="YGL226C-A"
/experiment="EXISTENCE:direct assay:GO:0005198 structural
molecule activity [PMID:12805367]"
/experiment="EXISTENCE:direct assay:GO:0008250
oligosaccharyltransferase complex
[PMID:12805367|PMID:10358084]"
/experiment="EXISTENCE:mutant phenotype:GO:0006487 protein
N-linked glycosylation [PMID:9135133]"
/experiment="EXISTENCE:physical interaction:GO:0006487
protein N-linked glycosylation [PMID:9405463]"
/experiment="EXISTENCE:physical interaction:GO:0008250
oligosaccharyltransferase complex [PMID:9405463]"
/note="Zeta subunit of the oligosaccharyltransferase
complex of the ER lumen; complex catalyzes
asparagine-linked glycosylation of newly synthesized
proteins"
/codon_start=1
/product="dolichyl-diphosphooligosaccharide--protein
glycotransferase subunit"
/protein_id="NP_011288.1"
/db_xref="GeneID:852625"
/db_xref="SGD:S000003194"
/translation="MTYEQLYKEFHSSKSFQPFIHLDTQPKFAICGLIVTLAVLSSAL
FAVGSKSSYIKKLFFYTILSVIGSLFAGLTTVFASNSFGVYV"
gene <73340..>73711
/gene="MTC3"
/locus_tag="YGL226W"
/db_xref="GeneID:852626"
mRNA <73340..>73711
/gene="MTC3"
/locus_tag="YGL226W"
/product="Mtc3p"
/transcript_id="NM_001181092.1"
/db_xref="GeneID:852626"
CDS 73340..73711
/gene="MTC3"
/locus_tag="YGL226W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095|PMID:24769239]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to the mitochondrion; mtc3
is synthetically sick with cdc13-1"
/codon_start=1
/product="Mtc3p"
/protein_id="NP_011289.1"
/db_xref="GeneID:852626"
/db_xref="SGD:S000003195"
/translation="MMGRNGIRLALKRSFSTYQPPVVEITNITKLWPTLRPEVRDEIK
EYLRWRMQEDWRHIPLEETKAAYFLSYGPCGGRSKGNEWNVGYTGMRIVFNLVLFGGA
ATAFYNWKQDKKLEEQLRDLV"
gene complement(73829..73902)
/locus_tag="YNCG0002C"
/db_xref="GeneID:852627"
tRNA complement(73829..73902)
/locus_tag="YNCG0002C"
/product="tRNA-Val"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Valine tRNA (tRNA-Val), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:852627"
/db_xref="SGD:S000006757"
gene <76894..>77907
/gene="VRG4"
/locus_tag="YGL225W"
/gene_synonym="GOG5; LDB3; VAN2; VIG4"
/db_xref="GeneID:852647"
mRNA <76894..>77907
/gene="VRG4"
/locus_tag="YGL225W"
/gene_synonym="GOG5; LDB3; VAN2; VIG4"
/product="GDP-mannose transporter"
/transcript_id="NM_001181090.1"
/db_xref="GeneID:852647"
CDS 76894..77907
/gene="VRG4"
/locus_tag="YGL225W"
/gene_synonym="GOG5; LDB3; VAN2; VIG4"
/experiment="EXISTENCE:direct assay:GO:0005458 GDP-mannose
transmembrane transporter activity [PMID:20576760]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:9395539]"
/experiment="EXISTENCE:mutant phenotype:GO:0005458
GDP-mannose transmembrane transporter activity
[PMID:9395539]"
/experiment="EXISTENCE:mutant phenotype:GO:1990570
GDP-mannose transmembrane transport [PMID:9395539]"
/note="Golgi GDP-mannose transporter; regulates Golgi
function and glycosylation in Golgi; VRG4 has a paralog,
HVG1, that arose from the whole genome duplication"
/codon_start=1
/product="GDP-mannose transporter"
/protein_id="NP_011290.1"
/db_xref="GeneID:852647"
/db_xref="SGD:S000003193"
/translation="MSELKTGHAGHNPWASVANSGPISILSYCGSSILMTVTNKFVVN
LKDFNMNFVMLFVQSLVCTITLIILRILGYAKFRSLNKTDAKNWFPISFLLVLMIYTS
SKALQYLAVPIYTIFKNLTIILIAYGEVLFFGGSVTSMELSSFLLMVLSSVVATWGDQ
QAVAAKAASLAEGAAGAVASFNPGYFWMFTNCITSALFVLIMRKRIKLTNFKDFDTMF
YNNVLALPILLLFSFCVEDWSSVNLTNNFSNDSLTAMIISGVASVGISYCSGWCVRVT
SSTTYSMVGALNKLPIALSGLIFFDAPRNFLSILSIFIGFLSGIIYAVAKQKKQQAQP
LRK"
gene complement(<78015..>78857)
/gene="SDT1"
/locus_tag="YGL224C"
/gene_synonym="SSM1"
/db_xref="GeneID:852648"
mRNA complement(<78015..>78857)
/gene="SDT1"
/locus_tag="YGL224C"
/gene_synonym="SSM1"
/product="nucleotidase"
/transcript_id="NM_001181089.1"
/db_xref="GeneID:852648"
CDS complement(78015..78857)
/gene="SDT1"
/locus_tag="YGL224C"
/gene_synonym="SSM1"
/experiment="EXISTENCE:direct assay:GO:0008252
nucleotidase activity [PMID:11934891]"
/experiment="EXISTENCE:mutant phenotype:GO:0006206
pyrimidine nucleobase metabolic process [PMID:11934891]"
/note="Pyrimidine nucleotidase; responsible for production
of nicotinamide riboside and nicotinic acid riboside;
overexpression suppresses the 6-AU sensitivity of
transcription elongation factor S-II, as well as
resistance to other pyrimidine derivatives; SDT1 has a
paralog, PHM8, that arose from the whole genome
duplication"
/codon_start=1
/product="nucleotidase"
/protein_id="NP_011291.1"
/db_xref="GeneID:852648"
/db_xref="SGD:S000003192"
/translation="MTVEYTASDLATYQNEVNEQIAKNKAHLESLTHPGSKVTFPIDQ
DISATPQNPNLKVFFFDIDNCLYKSSTRIHDLMQQSILRFFQTHLKLSPEDAHVLNNS
YYKEYGLAIRGLVMFHKVNALEYNRLVDDSLPLQDILKPDIPLRNMLLRLRQSGKIDK
LWLFTNAYKNHAIRCLRLLGIADLFDGLTYCDYSRTDTLVCKPHVKAFEKAMKESGLA
RYENAYFIDDSGKNIETGIKLGMKTCIHLVENEVNEILGQTPEGAIVISDILELPHVV
SDLF"
gene complement(<79112..>80365)
/gene="COG1"
/locus_tag="YGL223C"
/gene_synonym="COD3; LDB11; SEC36"
/db_xref="GeneID:852649"
mRNA complement(<79112..>80365)
/gene="COG1"
/locus_tag="YGL223C"
/gene_synonym="COD3; LDB11; SEC36"
/product="Golgi transport complex subunit COG1"
/transcript_id="NM_001181088.1"
/db_xref="GeneID:852649"
CDS complement(79112..80365)
/gene="COG1"
/locus_tag="YGL223C"
/gene_synonym="COD3; LDB11; SEC36"
/experiment="EXISTENCE:mutant phenotype:GO:0000301
retrograde transport, vesicle recycling within Golgi
[PMID:11703943]"
/experiment="EXISTENCE:mutant phenotype:GO:0006891
intra-Golgi vesicle-mediated transport [PMID:11703943]"
/experiment="EXISTENCE:mutant phenotype:GO:0007030 Golgi
organization [PMID:15932880]"
/experiment="EXISTENCE:mutant phenotype:GO:0017119 Golgi
transport complex [PMID:11703943]"
/experiment="EXISTENCE:mutant phenotype:GO:0030674
protein-macromolecule adaptor activity [PMID:15932880]"
/experiment="EXISTENCE:mutant phenotype:GO:0032258
cytoplasm to vacuole targeting by the Cvt pathway
[PMID:20065092]"
/note="Essential component of the conserved oligomeric
Golgi complex; a cytosolic tethering complex (Cog1p
through Cog8p) that functions in protein trafficking to
mediate fusion of transport vesicles to Golgi
compartments"
/codon_start=1
/product="Golgi transport complex subunit COG1"
/protein_id="NP_011292.1"
/db_xref="GeneID:852649"
/db_xref="SGD:S000003191"
/translation="MDEVLPLFRDSHIPQIKDYQLELQNDLTKTNEAFQKNLLKNYNK
ILSLTDSVNDLSLNLKNVDQDFKSLCFNDEKFQLNKLTPLPYQTTTHISPPRDEEKVS
IPSQNILVISNWTISINNFCNRIVTSTTPSRIFDELLLNFHELSLIPVPSKFEALVKD
KCCRLQKFLVDSMKTLNLTLLQWVKLYNLLNTEFSSKWDDDLLSIFNESLFETLFNDN
VQALLISSANSKDHQYHSNQQYKDAIVVDFVNSSTFRDHLIRRTVKEINTHLDTLSTL
RAKLKEPETLHKLDIFHDNDTNLNDGTVSPLDDDALKQYIDTAVFYSKGLTNDTTLQI
YQTVQPTIEILQNLELYKCPQETLTDLRNKLITQLQEFKTQISSRLPSPLENSTSVVD
DFITSYNNHNLLQLVIDQITQLRQQ"
gene complement(<80659..>81186)
/gene="EDC1"
/locus_tag="YGL222C"
/db_xref="GeneID:852650"
mRNA complement(<80659..>81186)
/gene="EDC1"
/locus_tag="YGL222C"
/product="Edc1p"
/transcript_id="NM_001181087.1"
/db_xref="GeneID:852650"
CDS complement(80659..81186)
/gene="EDC1"
/locus_tag="YGL222C"
/experiment="EXISTENCE:direct assay:GO:0000290
deadenylation-dependent decapping of nuclear-transcribed
mRNA [PMID:12554867]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:12554867]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:19682251]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:19682251|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0098745 RNA
decapping complex [PMID:11139489]"
/experiment="EXISTENCE:mutant phenotype:GO:0032056
positive regulation of translation in response to stress
[PMID:19682251]"
/experiment="EXISTENCE:physical interaction:GO:0098745 RNA
decapping complex [PMID:11139489]"
/note="RNA-binding protein that activates mRNA decapping
directly; binds to mRNA substrate and enhances activity of
decapping proteins Dcp1p and Dcp2p; has a role in
translation during heat stress; protein becomes more
abundant and forms cytoplasmic foci in response to DNA
replication stress; EDC1 has a paralog, EDC2, that arose
from the whole genome duplication"
/codon_start=1
/product="Edc1p"
/protein_id="NP_011293.1"
/db_xref="GeneID:852650"
/db_xref="SGD:S000003190"
/translation="MSTDTMYFNSSRLLPSAGRNKTNNLIKQKTRNNRARGNAAKNAN
NNNYITDIPPPQTLPNGQKPNFGHSSNKKPSFNQKKHSPPSSPSSTTTLGKKNRQNNK
ETPRQNNKDDTRLLSQNLKNLLLNQKQSPHGSQGIIPMGCNGSAKKLSHSYAGSTFAT
NGPREAKNLPKPSFL"
gene complement(<81426..>82292)
/gene="NIF3"
/locus_tag="YGL221C"
/db_xref="GeneID:852651"
mRNA complement(<81426..>82292)
/gene="NIF3"
/locus_tag="YGL221C"
/product="uncharacterized protein"
/transcript_id="NM_001181086.1"
/db_xref="GeneID:852651"
CDS complement(81426..82292)
/gene="NIF3"
/locus_tag="YGL221C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:17034789|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:17034789|PMID:24769239|PMID:16823961|PMID:14562095]"
/note="hypothetical protein; the authentic, non-tagged
protein is detected in highly purified mitochondria in
high-throughput studies"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011294.1"
/db_xref="GeneID:852651"
/db_xref="SGD:S000003189"
/translation="MSRAITRAQLDKLVRSITKFYPQKYADKSWDNTGLLIDCSTAQV
TTADANAKTKVLLTVDLTKSVAQEAVDANCNVIVAYHPFIFPSWNRLSPHTNPQHETA
IKLIQYGISVYCPHTAVDAARGGVNDWLVRGLNNGENVAKSYALETVSGETDDLIGYG
RFVEFNKDISLEQIVKNVKRVLRVPYVQVASLAAPSAWNQLKIKKVAVCAGSGSGVFK
QLKEDVDLYYTGEMSHHEVLKWKEMGKTVIVCNHSNTERGFLQDVMKGLLQDEGHEVV
VSKMDCDPLTVA"
gene <82374..>82736
/gene="BOL2"
/locus_tag="YGL220W"
/gene_synonym="AIM15; FRA2"
/db_xref="GeneID:852652"
mRNA <82374..>82736
/gene="BOL2"
/locus_tag="YGL220W"
/gene_synonym="AIM15; FRA2"
/product="Bol2p"
/transcript_id="NM_001181085.1"
/db_xref="GeneID:852652"
CDS 82374..82736
/gene="BOL2"
/locus_tag="YGL220W"
/gene_synonym="AIM15; FRA2"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:18281282]"
/experiment="EXISTENCE:direct assay:GO:0051536 iron-sulfur
cluster binding [PMID:31493153]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:18281282]"
/experiment="EXISTENCE:mutant phenotype:GO:0071281
cellular response to iron ion [PMID:18281282]"
/note="Cytosolic protein involved in repression of iron
regulon transcription; forms an iron-independent complex
with Fra1p, Grx3p, and Grx4p; null mutant fails to repress
the iron regulon and is sensitive to nickel; sequence
similarity to human BOLA family member, BOLA2"
/codon_start=1
/product="Bol2p"
/protein_id="NP_011295.1"
/db_xref="GeneID:852652"
/db_xref="SGD:S000003188"
/translation="MTGERIEKVKINDEFAKSHFLTTQWRETKRQRHYKMPVTEQGLR
ERIESAIPQVYHIIVTDLSYGCGQSFDIVVVSDFFQGKSKLMRSRAVNKAVKEELQEI
HAFSCKCYTEEEWSKIVV"
gene complement(<82878..>84257)
/gene="MDM34"
/locus_tag="YGL219C"
/gene_synonym="MMM2"
/db_xref="GeneID:852654"
mRNA complement(<82878..>84257)
/gene="MDM34"
/locus_tag="YGL219C"
/gene_synonym="MMM2"
/product="ERMES complex subunit MDM34"
/transcript_id="NM_001181084.1"
/db_xref="GeneID:852654"
CDS complement(82878..84257)
/gene="MDM34"
/locus_tag="YGL219C"
/gene_synonym="MMM2"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane
[PMID:14981098|PMID:16407407]"
/experiment="EXISTENCE:direct assay:GO:0044233
mitochondria-associated endoplasmic reticulum membrane
contact site [PMID:37366411]"
/experiment="EXISTENCE:genetic interaction:GO:0015914
phospholipid transport [PMID:19556461]"
/experiment="EXISTENCE:mutant phenotype:GO:0007005
mitochondrion organization [PMID:11907266]"
/experiment="EXISTENCE:mutant phenotype:GO:1990456
mitochondrion-endoplasmic reticulum membrane tethering
[PMID:19556461]"
/experiment="EXISTENCE:physical interaction:GO:0032865
ERMES complex [PMID:21945531]"
/note="Mitochondrial component of the ERMES complex; links
the ER to mitochondria and may promote inter-organellar
calcium and phospholipid exchange as well as coordinating
mitochondrial DNA replication and growth; required for
mitophagy; ERMES complex is often co-localized with
peroxisomes and with concentrated areas of pyruvate
dehydrogenase"
/codon_start=1
/product="ERMES complex subunit MDM34"
/protein_id="NP_011296.1"
/db_xref="GeneID:852654"
/db_xref="SGD:S000003187"
/translation="MSFRFNEAVFGDNSFNERIREKLSTALNSPSKKKLDILKSGIKV
QKVDFPTIPQLEILDLDIITQPKSLAKGICKISCKDAMLRIQTVIESNLLLINEQDTP
SFTMPQLINNGSFTIPITMTFSSIELEAITNIFVKNPGIGISFNDVDLDFKFDCSVKI
LQSTIERRLKESMHVVFKDVLPSLIFNTSQNWFTNRGESTSTIPGKREHHHQQTTMSR
NVILDGSDFQELSPINMLRLSSIVSSRSTLSLHSTVMNSLSAIPGCLERQNLYRFISR
MPSLNNYYSSQSFPQPKSSTVSSKQLVKPFYCSHNLLPKTVLDSSQYDLATITKIQSR
LFDRSNSNDDNAKPRRRKIKCKKTRTPSNLQSQGEQAVDDSTAIETVTSTPVQTPIPE
LEEQSPPYLKTTVSIRDKYVIPEKISLNLDSKKDTSKKKPFYFIGLNSQEPSNNWKWG
MEDSPPPYH"
gene <84885..>87302
/gene="KIP3"
/locus_tag="YGL216W"
/db_xref="GeneID:852655"
mRNA <84885..>87302
/gene="KIP3"
/locus_tag="YGL216W"
/product="tubulin-dependent ATPase KIP3"
/transcript_id="NM_001181081.1"
/db_xref="GeneID:852655"
CDS 84885..87302
/gene="KIP3"
/locus_tag="YGL216W"
/experiment="EXISTENCE:direct assay:GO:0000776 kinetochore
[PMID:16533946]"
/experiment="EXISTENCE:direct assay:GO:0005876 spindle
microtubule [PMID:16533946]"
/experiment="EXISTENCE:direct assay:GO:0005880 nuclear
microtubule [PMID:9693366]"
/experiment="EXISTENCE:direct assay:GO:0005881 cytoplasmic
microtubule [PMID:9693366]"
/experiment="EXISTENCE:direct assay:GO:0008574
plus-end-directed microtubule motor activity
[PMID:16906145|PMID:16906148]"
/experiment="EXISTENCE:direct assay:GO:0070462 plus-end
specific microtubule depolymerization
[PMID:16906148|PMID:16906145]"
/experiment="EXISTENCE:direct assay:GO:0070463
tubulin-dependent ATPase activity [PMID:16906148]"
/experiment="EXISTENCE:direct assay:GO:1990023 mitotic
spindle midzone [PMID:16533946]"
/experiment="EXISTENCE:mutant phenotype:GO:0000132
establishment of mitotic spindle orientation
[PMID:9281582|PMID:16906148]"
/experiment="EXISTENCE:mutant phenotype:GO:0007020
microtubule nucleation [PMID:33752231]"
/experiment="EXISTENCE:mutant phenotype:GO:0007052 mitotic
spindle organization [PMID:10525539]"
/experiment="EXISTENCE:mutant phenotype:GO:0007079 mitotic
chromosome movement towards spindle pole [PMID:16533946]"
/experiment="EXISTENCE:mutant phenotype:GO:0030473 nuclear
migration along microtubule [PMID:9693366]"
/experiment="EXISTENCE:mutant phenotype:GO:0031115
negative regulation of microtubule polymerization
[PMID:24616221]"
/experiment="EXISTENCE:mutant phenotype:GO:0032888
regulation of mitotic spindle elongation [PMID:24616221]"
/experiment="EXISTENCE:mutant phenotype:GO:0045144 meiotic
sister chromatid segregation [PMID:31964755]"
/experiment="EXISTENCE:mutant phenotype:GO:0051228 mitotic
spindle disassembly [PMID:23851487|PMID:9813090]"
/experiment="EXISTENCE:mutant phenotype:GO:0090307 mitotic
spindle assembly [PMID:23851487]"
/experiment="EXISTENCE:mutant phenotype:GO:0099606
microtubule plus-end directed mitotic chromosome migration
[PMID:35791811]"
/note="Kinesin-related antiparallel sliding motor protein;
involved in mitotic spindle positioning; sliding activity
promotes bipolar spindle assembly and maintenance of
genome stability; inhibits spindle elongation,
destabilizing late anaphase spindle microtubules (MTs)
that polymerize beyond the midzone; required for plus-end
directed kinetochore movement along metaphase MTs enabling
chromosome biorientation and congression; involved in
chromosome movement towards the spindle pole during
anaphase A"
/codon_start=1
/product="tubulin-dependent ATPase KIP3"
/protein_id="NP_011299.1"
/db_xref="GeneID:852655"
/db_xref="SGD:S000003184"
/translation="MNVPETRQSSIVVAIRVRPFTSMEKTRLVNEASGAEANFPGLGD
SSLILPMSNNSDSDIDIDAEEGSTRSKRNSLLRRKVIRPEGIRKIVDCVDDRMLIFDP
ADRNPLNKVSDQVLNSMRARATKATASSINNSNATNKFSSQRRRHGGEIKFVFDKLFD
ETSSQARVYKETTSPLLDSVLDGFNSTVFAYGATGCGKTYTVSGTPSQPGIIFLAMEE
LFNKITDLKDEKDFEISLSYLEIYNERIRDLLKPETPSKRLVIREDTQNHIKVANLSY
HHPNTVEDVMDLVVQGNINRTTSPTEANEVSSRSHAVLQIHIMQTNKLVDLTSQHTFA
TLSIIDLAGSERAAATRNRGIRLHEGANINRSLLALGNCINALCLNDGSRSCHIPYRD
SKLTRLLKFSLGGNCKTVMIVCISPSSSHYDETLNTLKYANRAKEIKTKIIRNQQSLS
RHVGSYLKMITEQKRQIEELREREEKMISLKLTKYKLNKEKIQLAINECVNRVQQTYA
GVETYQVAKTLKSLILCKRRFLQMVKLEVDNLILLFEREESTAAEMQPVISNCRMISG
QLYNKIHELEMKFDETDTLSSVIHQVHSIDLNKLREMEDWDETYDLVYLESCLNQISE
LQRNEILVNSSIMTEKLMSDPGLNSRFKFLSKWLMNRTPNIESIIQDLVHIDEEFESF
ARTFIANPDSNFTNTNINIINTTAADLAVPAETLQRQNFSQKKVKWTSPDLSPSPMIE
PQPELEPELHQDQDAIASEVDVSMQDTTFNEQGPSTPSAPTTAVPRRKMRSSLLTHQS
LLATARK"
gene <87981..>89339
/gene="CLG1"
/locus_tag="YGL215W"
/db_xref="GeneID:852657"
mRNA <87981..>89339
/gene="CLG1"
/locus_tag="YGL215W"
/product="Clg1p"
/transcript_id="NM_001181080.1"
/db_xref="GeneID:852657"
CDS 87981..89339
/gene="CLG1"
/locus_tag="YGL215W"
/experiment="EXISTENCE:direct assay:GO:0016538
cyclin-dependent protein serine/threonine kinase regulator
activity [PMID:9032248]"
/experiment="EXISTENCE:mutant phenotype:GO:0016239
positive regulation of macroautophagy [PMID:20417603]"
/experiment="EXISTENCE:physical interaction:GO:0000307
cyclin-dependent protein kinase holoenzyme complex
[PMID:9032248]"
/note="Cyclin-like protein that interacts with Pho85p; has
sequence similarity to G1 cyclins PCL1 and PCL2"
/codon_start=1
/product="Clg1p"
/protein_id="NP_011300.1"
/db_xref="GeneID:852657"
/db_xref="SGD:S000003183"
/translation="MANTFKYYPETMGNSSGYPISLPFPKGSATSAVNVARQLPKYLG
HVPSQSVHTQLPSMASLGYFNQPSSTYYAPPAPLQQHQQPPILPPPGLMYTSNNNSNV
IPPPVQMIRDGQQQPQQSNQVNGGVSENLDYDISIMSKFIMENAFVAFNANYSTDDQT
TDLFFKGISSVLNATRLPSATIFLAIDYLFKYINKLSNGIHSIGGNSINIIYQNTMIA
FILANKFNDDKTFTNNSWSQATGILINVINDFERQWLRIFNWELYDSAFLYFEFVKNF
EIFKQNQLKPAVAVPTLLSPIVNVGDTRNVNFNLKPTSTNNLLSPVSNYETPMLMPHN
MFSSPSYQSNSRSEFSSMNGYYNYYNYNQPRLNYYQQFPNIYSSPISETQFDYDFYNF
SSQQQQQQQKQHSLLPAAPQLPPPHVHNQSYGHHLGWKSMDDTINHSRFERNYFPYSA
VY"
gene complement(<90054..>91247)
/gene="SKI8"
/locus_tag="YGL213C"
/gene_synonym="REC103"
/db_xref="GeneID:852659"
mRNA complement(<90054..>91247)
/gene="SKI8"
/locus_tag="YGL213C"
/gene_synonym="REC103"
/product="SKI complex subunit WD repeat protein SKI8"
/transcript_id="NM_001181078.1"
/db_xref="GeneID:852659"
CDS complement(90054..91247)
/gene="SKI8"
/locus_tag="YGL213C"
/gene_synonym="REC103"
/experiment="EXISTENCE:direct assay:GO:0000228 nuclear
chromosome [PMID:14992724]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14992724]"
/experiment="EXISTENCE:direct assay:GO:0055087 Ski complex
[PMID:10744028|PMID:18042677]"
/experiment="EXISTENCE:genetic interaction:GO:0007131
reciprocal meiotic recombination [PMID:14992724]"
/experiment="EXISTENCE:genetic interaction:GO:0070478
nuclear-transcribed mRNA catabolic process, 3'-5'
exonucleolytic nonsense-mediated decay [PMID:12881429]"
/experiment="EXISTENCE:mutant phenotype:GO:0000956
nuclear-transcribed mRNA catabolic process [PMID:9482746]"
/experiment="EXISTENCE:mutant phenotype:GO:0007131
reciprocal meiotic recombination [PMID:14992724]"
/experiment="EXISTENCE:mutant phenotype:GO:0065003
protein-containing complex assembly [PMID:14992724]"
/experiment="EXISTENCE:mutant phenotype:GO:0065004
protein-DNA complex assembly [PMID:14992724]"
/experiment="EXISTENCE:mutant phenotype:GO:0070478
nuclear-transcribed mRNA catabolic process, 3'-5'
exonucleolytic nonsense-mediated decay [PMID:12881429]"
/experiment="EXISTENCE:mutant phenotype:GO:0070481
nuclear-transcribed mRNA catabolic process, non-stop decay
[PMID:11910110|PMID:17660569]"
/experiment="EXISTENCE:physical interaction:GO:0007131
reciprocal meiotic recombination [PMID:14992724]"
/experiment="EXISTENCE:physical interaction:GO:0055087 Ski
complex [PMID:18042677]"
/note="Ski complex component and WD-repeat protein;
mediates 3'-5' RNA degradation by the cytoplasmic exosome;
also required for meiotic double-strand break
recombination; null mutants have superkiller phenotype"
/codon_start=1
/product="SKI complex subunit WD repeat protein SKI8"
/protein_id="NP_011302.1"
/db_xref="GeneID:852659"
/db_xref="SGD:S000003181"
/translation="MSKVFIATANAGKAHDADIFSVSACNSFTVSCSGDGYLKVWDNK
LLDNENPKDKSYSHFVHKSGLHHVDVLQAIERDAFELCLVATTSFSGDLLFYRITRED
ETKKVIFEKLDLLDSDMKKHSFWALKWGASNDRLLSHRLVATDVKGTTYIWKFHPFAD
ESNSLTLNWSPTLELQGTVESPMTPSQFATSVDISERGLIATGFNNGTVQISELSTLR
PLYNFESQHSMINNSNSIRSVKFSPQGSLLAIAHDSNSFGCITLYETEFGERIGSLSV
PTHSSQASLGEFAHSSWVMSLSFNDSGETLCSAGWDGKLRFWDVKTKERITTLNMHCD
DIEIEEDILAVDEHGDSLAEPGVFDVKFLKKGWRSGMGADLNESLCCVCLDRSIRWFR
EAGGK"
gene <91432..>92382
/gene="VAM7"
/locus_tag="YGL212W"
/gene_synonym="VPL24; VPS43"
/db_xref="GeneID:852660"
mRNA <91432..>92382
/gene="VAM7"
/locus_tag="YGL212W"
/gene_synonym="VPL24; VPS43"
/product="Vam7p"
/transcript_id="NM_001181077.1"
/db_xref="GeneID:852660"
CDS 91432..92382
/gene="VAM7"
/locus_tag="YGL212W"
/gene_synonym="VPL24; VPS43"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane
[PMID:16984909|PMID:11433291|PMID:26928762|PMID:27881666]"
/experiment="EXISTENCE:direct assay:GO:0000407 phagophore
assembly site [PMID:26774783]"
/experiment="EXISTENCE:direct assay:GO:0005484 SNAP
receptor activity [PMID:19805279]"
/experiment="EXISTENCE:direct assay:GO:0006906 vesicle
fusion [PMID:11001046]"
/experiment="EXISTENCE:direct assay:GO:0032266
phosphatidylinositol-3-phosphate binding [PMID:11557775]"
/experiment="EXISTENCE:direct assay:GO:0042144 vacuole
fusion, non-autophagic [PMID:17400548]"
/experiment="EXISTENCE:direct assay:GO:0070300
phosphatidic acid binding [PMID:27365394]"
/experiment="EXISTENCE:mutant phenotype:GO:0000329
fungal-type vacuole membrane [PMID:16984909]"
/experiment="EXISTENCE:mutant phenotype:GO:0016236
macroautophagy [PMID:19793921]"
/experiment="EXISTENCE:mutant phenotype:GO:0031201 SNARE
complex [PMID:9628864]"
/experiment="EXISTENCE:mutant phenotype:GO:0032258
cytoplasm to vacuole targeting by the Cvt pathway
[PMID:19793921]"
/experiment="EXISTENCE:mutant phenotype:GO:0032266
phosphatidylinositol-3-phosphate binding [PMID:27365394]"
/experiment="EXISTENCE:mutant phenotype:GO:0034727
piecemeal microautophagy of the nucleus [PMID:18701704]"
/experiment="EXISTENCE:mutant phenotype:GO:0042144 vacuole
fusion, non-autophagic [PMID:17400548]"
/experiment="EXISTENCE:mutant phenotype:GO:0061909
autophagosome-lysosome fusion [PMID:26774783]"
/experiment="EXISTENCE:physical interaction:GO:0031201
SNARE complex [PMID:9628864]"
/note="Vacuolar membrane protein and Qc class t-SNARE;
functions as a SNAP receptor; involved with Vam3p in
vacuolar protein trafficking; has an N-terminal PX domain
(phosphoinositide-binding module) that binds both
PtdIns-3-P and phosphatidic acid, and mediates membrane
binding; localizes to both the vacuolar membrane and the
phagophore assembly site; SNAP-25 homolog; protein
abundance increases in response to DNA replication stress"
/codon_start=1
/product="Vam7p"
/protein_id="NP_011303.1"
/db_xref="GeneID:852660"
/db_xref="SGD:S000003180"
/translation="MAANSVGKMSEKLRIKVDDVKINPKYVLYGVSTPNKRLYKRYSE
FWKLKTRLERDVGSTIPYDFPEKPGVLDRRWQRRYDDPEMIDERRIGLERFLNELYND
RFDSRWRDTKIAQDFLQLSKPNVSQEKSQQHLETADEVGWDEMIRDIKLDLDKESDGT
PSVRGALRARTKLHKLRERLEQDVQKKSLPSTEVTRRAALLRSLLKECDDIGTANIAQ
DRGRLLGVATSDNSSTTEVQGRTNNDLQQGQMQMVRDQEQELVALHRIIQAQRGLALE
MNEELQTQNELLTALEDDVDNTGRRLQIANKKARHFNNSA"
gene <92512..>93591
/gene="NCS6"
/locus_tag="YGL211W"
/gene_synonym="TUC1; YGL210W-A"
/db_xref="GeneID:852661"
mRNA <92512..>93591
/gene="NCS6"
/locus_tag="YGL211W"
/gene_synonym="TUC1; YGL210W-A"
/product="Ncs6p"
/transcript_id="NM_001181076.1"
/db_xref="GeneID:852661"
CDS 92512..93591
/gene="NCS6"
/locus_tag="YGL211W"
/gene_synonym="TUC1; YGL210W-A"
/experiment="EXISTENCE:direct assay:GO:0000049 tRNA
binding [PMID:19145231]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:36164978]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:18664566]"
/experiment="EXISTENCE:mutant phenotype:GO:0002098 tRNA
wobble uridine modification [PMID:17592039|PMID:18755837]"
/experiment="EXISTENCE:mutant phenotype:GO:0002143 tRNA
wobble position uridine thiolation
[PMID:19145231|PMID:19151091|PMID:18664566]"
/experiment="EXISTENCE:mutant phenotype:GO:0032447 protein
urmylation [PMID:14551258]"
/note="Protein required for uridine thiolation of Gln,
Lys, and Glu tRNAs; required for the thiolation of uridine
at the wobble position of Gln, Lys, and Glu tRNAs; has a
role in urmylation and in invasive and pseudohyphal
growth; inhibits replication of Brome mosaic virus in S.
cerevisiae; localizes to cytosol and peroxisomes"
/codon_start=1
/product="Ncs6p"
/protein_id="NP_011304.2"
/db_xref="GeneID:852661"
/db_xref="SGD:S000003179"
/translation="MSFTAPSDPVNKPTKVKVSQLCELCHSRKALIRRPKNLSKLCKQ
CFCLVFETEIHNTIVANNLFQRGEKVAVGASGGKDSTVLAHMLKLLNDRYDYGIEIVL
LSIDEGIIGYRDDSLATVKRNQQQYGLPLEIFSFKDLYDWTMDEIVSVAGIRNSCTYC
GVFRRQSLDRGAAKLGISHVVTGHNADDMAETVLMNILRGDVARLEKSTAIITQSSGS
PIKRSKPFKYSYQKEIVLYAHYMKLDYFSTECTYAPEAFRGTAREYMKNLEAVRPSCI
IDIIQSGENLALKAKKSNAGKRVVKFVDGNRCARCGYLSSNNICKACMLLEGLEKSRA
QVAIENDTSADGAALKLRALEKLSF"
gene <93792..>94460
/gene="YPT32"
/locus_tag="YGL210W"
/db_xref="GeneID:852662"
mRNA <93792..>94460
/gene="YPT32"
/locus_tag="YGL210W"
/product="Rab family GTPase YPT32"
/transcript_id="NM_001181075.1"
/db_xref="GeneID:852662"
CDS 93792..94460
/gene="YPT32"
/locus_tag="YGL210W"
/experiment="EXISTENCE:direct assay:GO:0003924 GTPase
activity [PMID:11210571]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:16407407]"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:15537705]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:15537705]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0034498
early endosome to Golgi transport [PMID:15537705]"
/experiment="EXISTENCE:mutant phenotype:GO:0006887
exocytosis [PMID:9151665]"
/experiment="EXISTENCE:mutant phenotype:GO:0016192
vesicle-mediated transport [PMID:9151665]"
/note="Rab family GTPase involved in the exocytic pathway;
mediates intra-Golgi traffic or the budding of post-Golgi
vesicles from the trans-Golgi; protein abundance increases
in response to DNA replication stress; YPT32 has a
paralog, YPT31, that arose from the whole genome
duplication"
/codon_start=1
/product="Rab family GTPase YPT32"
/protein_id="NP_011305.1"
/db_xref="GeneID:852662"
/db_xref="SGD:S000003178"
/translation="MSNEDYGYDYDYLFKIVLIGDSGVGKSNLLSRFTTDEFNIESKS
TIGVEFATRTIEVENKKIKAQIWDTAGQERYRAITSAYYRGAVGALIVYDISKSSSYE
NCNHWLTELRENADDNVAVGLIGNKSDLAHLRAVPTDEAKNFAMENQMLFTETSALNS
DNVDKAFRELIVAIFQMVSKHQVDLSGSGTNNMGSNGAPKGPTISLTPAPKEDKKKKS
SNCC"
gene <95858..>97006
/gene="MIG2"
/locus_tag="YGL209W"
/gene_synonym="MLZ1"
/db_xref="GeneID:852663"
mRNA <95858..>97006
/gene="MIG2"
/locus_tag="YGL209W"
/gene_synonym="MLZ1"
/product="Mig2p"
/transcript_id="NM_001181074.1"
/db_xref="GeneID:852663"
CDS 95858..97006
/gene="MIG2"
/locus_tag="YGL209W"
/gene_synonym="MLZ1"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:8756637|PMID:9832517]"
/experiment="EXISTENCE:direct assay:GO:0001227 DNA-binding
transcription repressor activity, RNA polymerase
II-specific [PMID:9832517]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9832517|PMID:22842922|PMID:22353369]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:22353369]"
/experiment="EXISTENCE:genetic interaction:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:9858577]"
/experiment="EXISTENCE:genetic interaction:GO:0001227
DNA-binding transcription repressor activity, RNA
polymerase II-specific [PMID:9832517]"
/experiment="EXISTENCE:genetic interaction:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:9832517]"
/experiment="EXISTENCE:genetic interaction:GO:1900436
positive regulation of filamentous growth of a population
of unicellular organisms in response to starvation
[PMID:22904036]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:9858577]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:9832517]"
/note="Zinc finger transcriptional repressor; cooperates
with Mig1p in glucose-induced gene repression; under low
glucose conditions relocalizes to mitochondrion, where it
interacts with Ups1p, antagonizes mitochondrial fission
factor Dnm1p, indicative of a role in mitochondrial fusion
or regulating morphology; regulates filamentous growth in
response to glucose depletion; activated in stochastic
pulses of nuclear localization in response to low glucose"
/codon_start=1
/product="Mig2p"
/protein_id="NP_011306.1"
/db_xref="GeneID:852663"
/db_xref="SGD:S000003177"
/translation="MPKKQTNFPVDNENRPFRCDTCHRGFHRLEHKKRHLRTHTGEKP
HHCAFPGCGKSFSRSDELKRHMRTHTGQSQRRLKKASVQKQEFLTVSGIPTIASGVMI
HQPIPQVLPANMAINVQAVNGGNIIHAPNAVHPMVIPIMAQPAPIHASAASFQPATSP
MPISTYTPVPSQSFTSFQSSIGSIQSNSDVSSIFSNMNVRVNTPRSVPNSPNDGYLHQ
QHIPQQYQHQTASPSVAKQQKTFAHSLASALSTLQKRTPVSAPSTTIESPSSPSDSSH
TSASSSAISLPFSNAPSQLAVAKELESVYLDSNRYTTKTRRERAKFEIPEEQEEDTNN
SSSGSNEEEHESLDHESSKSRKKLSGVKLPPVRNLLKQIDVFNGPKRV"
gene <97338..>98585
/gene="SIP2"
/locus_tag="YGL208W"
/gene_synonym="SPM2"
/db_xref="GeneID:852664"
mRNA <97338..>98585
/gene="SIP2"
/locus_tag="YGL208W"
/gene_synonym="SPM2"
/product="Sip2p"
/transcript_id="NM_001181073.2"
/db_xref="GeneID:852664"
CDS 97338..98585
/gene="SIP2"
/locus_tag="YGL208W"
/gene_synonym="SPM2"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11331606]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:12562756]"
/experiment="EXISTENCE:direct assay:GO:0031588
nucleotide-activated protein kinase complex
[PMID:12393914]"
/experiment="EXISTENCE:genetic interaction:GO:0001403
invasive growth in response to glucose limitation
[PMID:12556493]"
/experiment="EXISTENCE:genetic interaction:GO:0004679
AMP-activated protein kinase activity [PMID:10990457]"
/experiment="EXISTENCE:genetic interaction:GO:0007165
signal transduction [PMID:10990457]"
/experiment="EXISTENCE:genetic interaction:GO:0043254
regulation of protein-containing complex assembly
[PMID:9121458]"
/experiment="EXISTENCE:mutant phenotype:GO:0042149
cellular response to glucose starvation [PMID:10921902]"
/experiment="EXISTENCE:physical interaction:GO:0031588
nucleotide-activated protein kinase complex
[PMID:9121458|PMID:7813428]"
/note="One of three alternate beta subunits of the Snf1
kinase complex; involved in the response to glucose
starvation; null mutants exhibit accelerated aging;
N-myristoylprotein localized to the cytoplasm and the
plasma membrane; SIP2 has a paralog, GAL83, that arose
from the whole genome duplication"
/codon_start=1
/product="Sip2p"
/protein_id="NP_011307.1"
/db_xref="GeneID:852664"
/db_xref="SGD:S000003176"
/translation="MGTTTSHPAQKKQTTKKCRAPIMSDVREKPSNAQGCEPQEMDAV
SKKVTELSLNKCSDSQDAGQPSREGSITKKKSTLLLRDEDEPTMPKLSVMETAVDTDS
GSSSTSDDEEGDIIAQTTEPKQDASPDDDRSGHSSPREEGQQQIRAKEASGGPSEIKS
SLMVPVEIRWQQGGSKVYVTGSFTKWRKMIGLIPDSDNNGSFHVKLRLLPGTHRFRFI
VDNELRVSDFLPTATDQMGNFVNYIEVRQPEKNPTNEKIRSKEADSMRPPTSDRSSIA
LQIGKDPDDFGDGYTRFHEDLSPRPPLEYTTDIPAVFTDPSVMERYYYTLDRQQSNTD
TSWLTPPQLPPQLENVILNKYYATQDQFNENNSGALPIPNHVVLNHLVTSSIKHNTLC
VASIVRYKQKYVTQILYTPIESS"
gene <98969..>102076
/gene="SPT16"
/locus_tag="YGL207W"
/gene_synonym="CDC68; SSF1"
/db_xref="GeneID:852665"
mRNA <98969..>102076
/gene="SPT16"
/locus_tag="YGL207W"
/gene_synonym="CDC68; SSF1"
/product="chromatin-remodeling protein SPT16"
/transcript_id="NM_001181072.1"
/db_xref="GeneID:852665"
CDS 98969..102076
/gene="SPT16"
/locus_tag="YGL207W"
/gene_synonym="CDC68; SSF1"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:10413469]"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:10413469]"
/experiment="EXISTENCE:direct assay:GO:0006325 chromatin
organization [PMID:19683499]"
/experiment="EXISTENCE:direct assay:GO:0006334 nucleosome
assembly [PMID:12952948|PMID:15082784]"
/experiment="EXISTENCE:direct assay:GO:0007063 regulation
of sister chromatid cohesion [PMID:31582854]"
/experiment="EXISTENCE:direct assay:GO:0031491 nucleosome
binding [PMID:11432837]"
/experiment="EXISTENCE:direct assay:GO:0042393 histone
binding [PMID:18089575]"
/experiment="EXISTENCE:direct assay:GO:0045899 positive
regulation of RNA polymerase II transcription
preinitiation complex assembly [PMID:15987999]"
/experiment="EXISTENCE:genetic interaction:GO:0035101 FACT
complex [PMID:11432837]"
/experiment="EXISTENCE:genetic interaction:GO:0060261
positive regulation of transcription initiation by RNA
polymerase II [PMID:15987999]"
/experiment="EXISTENCE:mutant phenotype:GO:0060261
positive regulation of transcription initiation by RNA
polymerase II [PMID:19574230]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:27226635]"
/experiment="EXISTENCE:physical interaction:GO:0006261
DNA-templated DNA replication [PMID:9199353]"
/experiment="EXISTENCE:physical interaction:GO:0035101
FACT complex [PMID:11432837]"
/note="Subunit of the heterodimeric FACT complex
(Spt16p-Pob3p); FACT associates with chromatin via
interaction with Nhp6Ap and Nhp6Bp, and reorganizes
nucleosomes to facilitate access to DNA by RNA and DNA
polymerases; specifically required for diauxic
shift-induced H2B deposition onto rDNA genes; mutations
cause reduced nucleosome occupancy over highly transcribed
regions; coregulates transcription with Mot1p through
preinitiation complex assembly and nucleosome
organization"
/codon_start=1
/product="chromatin-remodeling protein SPT16"
/protein_id="NP_011308.1"
/db_xref="GeneID:852665"
/db_xref="SGD:S000003175"
/translation="MEELNIDFDVFKKRIELLYSKYNEFEGSPNSLLFVLGSSNAENP
YQKTTILHNWLLSYEFPATLIALVPGKVIIITSSAKAKHLQKAIDLFKDPESKITLEL
WQRNNKEPELNKKLFDDVIALINSAGKTVGIPEKDSYQGKFMTEWNPVWEAAVKENEF
NVIDISLGLSKVWEVKDVNEQAFLSVSSKGSDKFMDLLSNEMVRAVDEELKITNAKLS
DKIENKIDDVKFLKQLSPDLSALCPPNYKFNFDLLDWTYSPIIQSGKKFDLRVSARST
NDQLYGNGCILASCGIRYNNYCSNITRTFLIDPSEEMANNYDFLLTLQKEIVTNILKP
GRTPKEVYESVIEYIEKTKPELVPNFTKNIGSLIGLEFRDSNFILNVKNDYRKIQRGD
CFNISFGFNNLKDSQSANNYALQLADTVQIPLDETEPPRFLTNYTKAKSQISFYFNNE
EEDNNKKKSSPATKVPSKPDRNSKILRTKLRGEARGGAEDAQKEQIRKENQKKLHEKL
EKNGLLRFSAADANGPDSEPRQYFKKYESYVRDSQLPTNIRDLRIHVDWKSQTIILPI
YGRPVPFHINSYKNGSKNEEGEYTYLRLNFNSPGSSGGISKKVEELPYEESADNQFVR
SITLRSKDGDRMSETFKQIADLKKEATKREQERKALADVVQQDKLIENKTGRTKRLDQ
IFVRPNPDTKRVPSTVFIHENGIRFQSPLRTDSRIDILFSNIKNLIFQSCKGELIVVI
HIHLKNPILMGKKKIQDVQFYREASDMSVDETGGGRRGQSRFRRYGDEDELEQEQEER
RKRAALDKEFKYFADAIAEASNGLLTVENTFRDLGFQGVPNRSAVFCMPTTDCLVQLI
EPPFLVINLEEVEICILERVQFGLKNFDMVFVYKDFNKPVTHINTVPIESLDFLKQWL
TDMDIPYTVSTINLNWATIMKSLQDDPYQFFLDGGWNFLATGSDDEASDESEEEVSEY
EASEDDVSDESAFSEDEEGSEVDDDISGDESEDYTGDESEEGEDWDELEKKAARADRG
ANFRD"
gene complement(<102543..>107504)
/gene="CHC1"
/locus_tag="YGL206C"
/gene_synonym="SWA5"
/db_xref="GeneID:852666"
mRNA complement(<102543..>107504)
/gene="CHC1"
/locus_tag="YGL206C"
/gene_synonym="SWA5"
/product="clathrin heavy chain"
/transcript_id="NM_001181071.1"
/db_xref="GeneID:852666"
CDS complement(102543..107504)
/gene="CHC1"
/locus_tag="YGL206C"
/gene_synonym="SWA5"
/experiment="EXISTENCE:direct assay:GO:0006897 endocytosis
[PMID:16643280]"
/experiment="EXISTENCE:genetic interaction:GO:0030866
cortical actin cytoskeleton organization [PMID:12181333]"
/experiment="EXISTENCE:mutant phenotype:GO:0006895 Golgi
to endosome transport [PMID:27062026]"
/experiment="EXISTENCE:mutant phenotype:GO:0006897
endocytosis [PMID:16643280|PMID:27062026]"
/experiment="EXISTENCE:mutant phenotype:GO:0030866
cortical actin cytoskeleton organization [PMID:12181333]"
/note="Clathrin heavy chain; subunit of the major coat
protein involved in intracellular protein transport and
endocytosis; the clathrin triskelion is a trimeric
molecule composed of three heavy chains that radiate from
a vertex and three light chains which bind noncovalently
near the vertex of the triskelion; the light chain (CLC1)
is thought to regulate function"
/codon_start=1
/product="clathrin heavy chain"
/protein_id="NP_011309.1"
/db_xref="GeneID:852666"
/db_xref="SGD:S000003174"
/translation="MSDLPIEFTELVDLMSLGISPQFLDFRSTTFESDHFVTVRETKD
GTNSVAIVDLAKGNEVTRKNMGGDSAIMHPSQMVISVRANGTIVQIFNLETKSKLKSF
TLDEPVIFWRWLSETTLGFVTARSILTSNVFDGNVNAKPQLLTLRHANLNNTQIINFV
ANKNLDWFAVVGILQENGRIAGRIQLFSKQRNISQAIDGHVAIFTNILLEGNGSTPVQ
VFVTGNRNATTGAGELRIIEIDHDASLPSQYQKETTDIFFPPDATNDFPIAVQVSEKY
GIIYLLTKYGFIHLYELETGTNLFVNRITAESVFTAAPYNHENGIACINKKGQVLAVE
ISTSQIVPYILNKLSNVALALIVATRGGLPGADDLFQKQFESLLLQNDYQNAAKVAAS
STSLRNQNTINRLKNIQAPPGAISPILLYFSTLLDKGKLNKEETIELARPVLQQDRKQ
LFEKWLKEDKLECSEELGDIVKPFDTTLALACYLRAGAHAKVISCLAELQQFEKIIPY
CQKVGYQPNFLVLISSLIRSSPDRASEFAVSLLQNPETASQIDIEKIADLFFSQNHIQ
QGTSLLLDALKGDTPDQGHLQTRVLEVNLLHAPQVADAILGNNIFSHYDKPTIASLSE
KAGLYQRALENYTDIKDIKRCVVHTNALPIDWLVGYFGKLNVEQSLACLKALMDNNIQ
ANIQTVVQVATKFSDLIGPSTLIKLFEDYNATEGLYYYLASLVNLTEDKDVVYKYIEA
AAKMKQYREIERIVKDNNVYDPERVKNFLKDANLEDQLPLVIVCDRFDFVHEMILYLY
KSQNLKFIETYVQQVNPSKTAQVVGALLDMDCDEAFIQSLLQSVLGQVPINELTTEVE
KRNRLKILLPFLEQSLSQGIQDQAVYNALAKIYIDSNNSPEKFLKENDQYDTLDVGHY
CEKRDPYLAYIAYEKGQNDDDLIRITNENSMYKYQARYLLERSDLDLWNKVLNQENIH
RRQLIDSVISVGIPELTDPEPVSLTVQAFMTNGLKLELIELLEKIILEPSPFNENVAL
QGLLLLSAIKYEPTKVSSYIEKLDNYDADEIAPLCIEHDLKEEAFEIYDKHEMYGKAL
KVLIEDIMSLDRAASYADKINTPELWSQIGTAQLDGLRIPDAIESYIKAEDPSNYENV
IDIAEQAGKYEELIPFLLMARKTLKEPKIDGALILAYAELNKIHEIENLLAGSNVANL
DHVGDKLFENKEYKAARLCYSAVSNYSKLASTLVYLGDYQAAVDTARKASNIKVWKLV
NDACIEKKEFKLAQICGLNLIVHAEELDELVERYESNGYFEELISLFEAGLGLERAHM
GMFTELAILYSKYEPDKTFEHLKLFWSRINIPKVIRAVEQAHLWSELVFLYAHYDEWD
NAALTLIEKSTKDLDHAYFKEVVVKVSNLEIYYKAINFYVKFHPSLLVDLLTSLTPRL
DIPRTVKIFSKSDNLPLIKPFLINVLPKNNSVVNQAYHDLMIEEEDYKALQDAVDSYD
KFDQLGLASRLESHKLIFFKKIGALLYRRNKKWAKSLSILKEEKLWKDAIETAAISQD
PKVVEALLTYFVETGNREGFVALLYAAYNLVRIEFVLEISWMNSLEDYIKPFEISIKK
EQNDSIKKITEELAKKSGSNEEHKDGQPLMLMNSAMNVQPTGF"
gene <108158..>110404
/gene="POX1"
/locus_tag="YGL205W"
/gene_synonym="FOX1"
/db_xref="GeneID:852667"
mRNA <108158..>110404
/gene="POX1"
/locus_tag="YGL205W"
/gene_synonym="FOX1"
/product="acyl-CoA oxidase"
/transcript_id="NM_001181070.1"
/db_xref="GeneID:852667"
CDS 108158..110404
/gene="POX1"
/locus_tag="YGL205W"
/gene_synonym="FOX1"
/EC_number="1.3.3.6"
/experiment="EXISTENCE:direct assay:GO:0005782 peroxisomal
matrix [PMID:11967269]"
/experiment="EXISTENCE:mutant phenotype:GO:0006635 fatty
acid beta-oxidation [PMID:12819196]"
/note="Fatty-acyl coenzyme A oxidase; involved in the
fatty acid beta-oxidation pathway; localized to the
peroxisomal matrix; component of a light sensing pathway,
converting light into a hydrogen peroxide signal,
modulating nucleo-cytoplasmic shuttling of
stress-responsive transcription factor, Msn2p"
/codon_start=1
/product="acyl-CoA oxidase"
/protein_id="NP_011310.1"
/db_xref="GeneID:852667"
/db_xref="SGD:S000003173"
/translation="MTRRTTINPDSVVLNPQKFIQKERADSKIKVDQVNTFLESSPER
RTLTHALIDQIVNDPILKTDTDYYDAKKMQEREITAKKIARLASYMEHDIKTVRKHFR
DTDLMKELQANDPDKASPLTNKDLFIFDKRLSLVANIDPQLGTRVGVHLGLFGNCIKG
NGTDEQIRYWLQERGATLMKGIYGCFAMTELGHGSNVAQLQTRAVYDKQNDTFVIDTP
DLTATKWWIGGAAHSATHAAVYARLIVEGKDYGVKTFVVPLRDPSTFQLLAGVSIGDI
GAKMGRDGIDNGWIQFRNVVIPREFMLSRFTKVVRSPDGSVTVKTEPQLDQISGYSAL
LSGRVNMVMDSFRFGSKFATIAVRYAVGRQQFAPRKGLSETQLIDYPLHQYRVLPQLC
VPYLVSPVAFKLMDNYYSTLDELYNASSSAYKAALVTVSKKLKNLFIDSASLKATNTW
LIATLIDELRQTCGGHGYSQYNGFGKGYDDWVVQCTWEGDNNVLSLTSAKSILKKFID
SATKGRFDNTLDVDSFSYLKPQYIGSVVSGEIKSGLKELGDYTEIWSITLIKLLAHIG
TLVEKSRSIDSVSKLLVLVSKFHALRCMLKTYYDKLNSRDSHISDEITKESMWNVYKL
FSLYFIDKHSGEFQQFKIFTPDQISKVVQPQLLALLPIVRKDCIGLTDSFELPDAMLN
SPIGYFDGDIYHNYFNEVCRNNPVEADGAGKPSYHALLSSMLGRGFEFDQKLGGAANA
EILSKINK"
gene complement(110625..110696)
/locus_tag="YNCG0003C"
/db_xref="GeneID:852668"
tRNA complement(110625..110696)
/locus_tag="YNCG0003C"
/product="tRNA-His"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0030371 translation
repressor activity [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043555 regulation
of translation in response to stress [PMID:27609601]"
/note="Histidine tRNA (tRNA-His), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:852668"
/db_xref="SGD:S000006595"
repeat_region 110855..111225
/note="Ty4 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006950"
repeat_region complement(111226..111505)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006935"
repeat_region 111506..111698
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006940"
gene complement(<111700..>112005)
/locus_tag="YGL204C"
/db_xref="GeneID:852669"
mRNA complement(<111700..>112005)
/locus_tag="YGL204C"
/product="uncharacterized protein"
/transcript_id="NM_001270746.1"
/db_xref="GeneID:852669"
CDS complement(111700..112005)
/locus_tag="YGL204C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/note="hypothetical protein; mRNA identified as translated
by ribosome profiling data; protein identified via
enrichment-based proteogenomics; SWAT-GFP and mCherry
fusion proteins localize to the endoplasmic reticulum"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001257675.1"
/db_xref="GeneID:852669"
/db_xref="SGD:S000003172"
/translation="MNGTDILRFLQSSPTISYSKHFILITACPLFVLGLLLLGLRTAM
FKQVRGKTTTSRNRGVIAAKLLVAWYLATIVMYIAKSEMWKYAFAVSLLLNSLALFF"
rep_origin 112079..112317
/note="ARS704; Autonomously Replicating Sequence"
/db_xref="SGD:S000118452"
gene complement(<112475..>114664)
/gene="KEX1"
/locus_tag="YGL203C"
/db_xref="GeneID:852670"
mRNA complement(<112475..>114664)
/gene="KEX1"
/locus_tag="YGL203C"
/product="serine-type carboxypeptidase"
/transcript_id="NM_001181068.1"
/db_xref="GeneID:852670"
CDS complement(112475..114664)
/gene="KEX1"
/locus_tag="YGL203C"
/EC_number="3.4.16.6"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0004185 serine-type
carboxypeptidase activity [PMID:2668738]"
/experiment="EXISTENCE:direct assay:GO:0005802 trans-Golgi
network [PMID:8308064]"
/experiment="EXISTENCE:mutant phenotype:GO:0006915
apoptotic process [PMID:23656787]"
/note="Cell death protease essential for
hypochlorite-induced apoptosis; involved in the processing
of killer toxin and alpha factor precursor; cleaves Lys
and Arg residues from the C-terminus of peptides and
proteins"
/codon_start=1
/product="serine-type carboxypeptidase"
/protein_id="NP_011312.1"
/db_xref="GeneID:852670"
/db_xref="SGD:S000003171"
/translation="MFYNRWLGTWLAMSALIRISVSLPSSEEYKVAYELLPGLSEVPD
PSNIPQMHAGHIPLRSEDADEQDSSDLEYFFWKFTNNDSNGNVDRPLIIWLNGGPGCS
SMDGALVESGPFRVNSDGKLYLNEGSWISKGDLLFIDQPTGTGFSVEQNKDEGKIDKN
KFDEDLEDVTKHFMDFLENYFKIFPEDLTRKIILSGESYAGQYIPFFANAILNHNKFS
KIDGDTYDLKALLIGNGWIDPNTQSLSYLPFAMEKKLIDESNPNFKHLTNAHENCQNL
INSASTDEAAHFSYQECENILNLLLSYTRESSQKGTADCLNMYNFNLKDSYPSCGMNW
PKDISFVSKFFSTPGVIDSLHLDSDKIDHWKECTNSVGTKLSNPISKPSIHLLPGLLE
SGIEIVLFNGDKDLICNNKGVLDTIDNLKWGGIKGFSDDAVSFDWIHKSKSTDDSEEF
SGYVKYDRNLTFVSVYNASHMVPFDKSLVSRGIVDIYSNDVMIIDNNGKNVMITTDDD
SDQDATTESGDKPKENLEEEEQEAQNEEGKEKEGNKDKDGDDDNDNDDDDEDDHNSEG
DDDDDDDDDEDDNNEKQSNQGLEDSRHKSSEYEQEEEEVEEFAEEISMYKHKAVVVTI
VTFLIVVLGVYAYDRRVRRKARHTILVDPNNRQHDSPNKTVSWADDLESGLGAEDDLE
QDEQLEGGAPISSTSNKAGSKLKTKKKKKYTSLPNTEIDESFEMTDF"
repeat_region 114939..115269
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006941"
gene 115488..115583
/locus_tag="YNCG0004W"
/db_xref="GeneID:852671"
tRNA join(115488..115524,115548..115583)
/locus_tag="YNCG0004W"
/product="tRNA-Lys"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE
analysis; thiolation of uridine at wobble position (34)
requires Ncs6p"
/db_xref="GeneID:852671"
/db_xref="SGD:S000006630"
gene <116059..>117561
/gene="ARO8"
/locus_tag="YGL202W"
/db_xref="GeneID:852672"
mRNA <116059..>117561
/gene="ARO8"
/locus_tag="YGL202W"
/product="bifunctional 2-aminoadipate
transaminase/aromatic-amino-acid:2-oxoglutarate
transaminase"
/transcript_id="NM_001181067.1"
/db_xref="GeneID:852672"
CDS 116059..117561
/gene="ARO8"
/locus_tag="YGL202W"
/EC_number="2.6.1.39"
/EC_number="2.6.1.57"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0047536
2-aminoadipate transaminase activity [PMID:19342587]"
/experiment="EXISTENCE:genetic interaction:GO:0006571
L-tyrosine biosynthetic process [PMID:9491082]"
/experiment="EXISTENCE:genetic interaction:GO:0009094
L-phenylalanine biosynthetic process [PMID:9491082]"
/experiment="EXISTENCE:mutant phenotype:GO:0008793
aromatic-amino-acid transaminase activity [PMID:9491082]"
/experiment="EXISTENCE:mutant phenotype:GO:0009072
aromatic amino acid metabolic process [PMID:9491083]"
/experiment="EXISTENCE:mutant phenotype:GO:0047536
2-aminoadipate transaminase activity [PMID:9491082]"
/note="Aromatic aminotransferase I; expression is
regulated by general control of amino acid biosynthesis;
involved with Aro9p in production of kynurenic acid to
detoxify excess tryptophan"
/codon_start=1
/product="bifunctional 2-aminoadipate
transaminase/aromatic-amino-acid:2-oxoglutarate
transaminase"
/protein_id="NP_011313.1"
/db_xref="GeneID:852672"
/db_xref="SGD:S000003170"
/translation="MTLPESKDFSYLFSDETNARKPSPLKTCIHLFQDPNIIFLGGGL
PLKDYFPWDNLSVDSPKPPFPQGIGAPIDEQNCIKYTVNKDYADKSANPSNDIPLSRA
LQYGFSAGQPELLNFIRDHTKIIHDLKYKDWDVLATAGNTNAWESTLRVFCNRGDVIL
VEAHSFSSSLASAEAQGVITFPVPIDADGIIPEKLAKVMENWTPGAPKPKLLYTIPTG
QNPTGTSIADHRKEAIYKIAQKYDFLIVEDEPYYFLQMNPYIKDLKEREKAQSSPKQD
HDEFLKSLANTFLSLDTEGRVIRMDSFSKVLAPGTRLGWITGSSKILKPYLSLHEMTI
QAPAGFTQVLVNATLSRWGQKGYLDWLLGLRHEYTLKRDCAIDALYKYLPQSDAFVIN
PPIAGMFFTVNIDASVHPEFKTKYNSDPYQLEQSLYHKVVERGVLVVPGSWFKSEGET
EPPQPAESKEVSNPNIIFFRGTYAAVSPEKLTEGLKRLGDTLYEEFGISK"
rep_origin 117561..117854
/note="ARS706; Autonomously Replicating Sequence"
/db_xref="SGD:S000114845"
gene complement(<117854..>120907)
/gene="MCM6"
/locus_tag="YGL201C"
/db_xref="GeneID:852673"
mRNA complement(<117854..>120907)
/gene="MCM6"
/locus_tag="YGL201C"
/product="MCM DNA helicase complex subunit MCM6"
/transcript_id="NM_001181066.1"
/db_xref="GeneID:852673"
CDS complement(117854..120907)
/gene="MCM6"
/locus_tag="YGL201C"
/EC_number="3.6.4.12"
/experiment="EXISTENCE:direct assay:GO:0003678 DNA
helicase activity [PMID:15723534]"
/experiment="EXISTENCE:direct assay:GO:0003688 DNA
replication origin binding [PMID:16824194|PMID:11756674]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10704410]"
/experiment="EXISTENCE:direct assay:GO:0005656 nuclear
pre-replicative complex [PMID:9335335|PMID:16824194]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:10704410]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:35563734]"
/experiment="EXISTENCE:direct assay:GO:0006260 DNA
replication [PMID:13679365|PMID:15723534]"
/experiment="EXISTENCE:direct assay:GO:0006267
pre-replicative complex assembly involved in nuclear cell
cycle DNA replication [PMID:16824194]"
/experiment="EXISTENCE:direct assay:GO:0009378 four-way
junction helicase activity [PMID:13679365]"
/experiment="EXISTENCE:direct assay:GO:0017116
single-stranded DNA helicase activity
[PMID:15723534|PMID:18657510]"
/experiment="EXISTENCE:direct assay:GO:0031261 DNA
replication preinitiation complex [PMID:9554851]"
/experiment="EXISTENCE:direct assay:GO:0042555 MCM complex
[PMID:12480933]"
/experiment="EXISTENCE:direct assay:GO:0043138 3'-5' DNA
helicase activity [PMID:13679365]"
/experiment="EXISTENCE:direct assay:GO:0071162 CMG complex
[PMID:26524492]"
/experiment="EXISTENCE:direct assay:GO:0097373 MCM core
complex [PMID:13679365]"
/experiment="EXISTENCE:mutant phenotype:GO:0000727
double-strand break repair via break-induced replication
[PMID:20516198]"
/experiment="EXISTENCE:mutant phenotype:GO:0006271 DNA
strand elongation involved in DNA replication
[PMID:10834843]"
/note="Protein involved in DNA replication; component of
the Mcm2-7 hexameric helicase complex that binds chromatin
as a part of the pre-replicative complex; forms a
subcomplex with Mcm4p and Mcm7p"
/codon_start=1
/product="MCM DNA helicase complex subunit MCM6"
/protein_id="NP_011314.2"
/db_xref="GeneID:852673"
/db_xref="SGD:S000003169"
/translation="MSSPFPADTPSSNRPSNSSPPPSSIGAGFGSSSGLDSQIGSRLH
FPSSSQPHVSNSQTGPFVNDSTQFSSQRLQTDGSATNDMEGNEPARSFKSRALNHVKK
VDDVTGEKVREAFEQFLEDFSVQSTDTGEVEKVYRAQIEFMKIYDLNTIYIDYQHLSM
RENGALAMAISEQYYRFLPFLQKGLRRVVRKYAPELLNTSDSLKRSEGDEGQADEDEQ
QDDDMNGSSLPRDSGSSAAPGNGTSAMATRSITTSTSPEQTERVFQISFFNLPTVHRI
RDIRSEKIGSLLSISGTVTRTSEVRPELYKASFTCDMCRAIVDNVEQSFKYTEPTFCP
NPSCENRAFWTLNVTRSRFLDWQKVRIQENANEIPTGSMPRTLDVILRGDSVERAKPG
DRCKFTGVEIVVPDVTQLGLPGVKPSSTLDTRGISKTTEGLNSGVTGLRSLGVRDLTY
KISFLACHVISIGSNIGASSPDANSNNRETELQMAANLQANNVYQDNERDQEVFLNSL
SSDEINELKEMVKDEHIYDKLVRSIAPAVFGHEAVKKGILLQMLGGVHKSTVEGIKLR
GDINICVVGDPSTSKSQFLKYVVGFAPRSVYTSGKASSAAGLTAAVVRDEEGGDYTIE
AGALMLADNGICCIDEFDKMDISDQVAIHEAMEQQTISIAKAGIHATLNARTSILAAA
NPVGGRYNRKLSLRGNLNMTAPIMSRFDLFFVILDDCNEKIDTELASHIVDLHMKRDE
AIEPPFSAEQLRRYIKYARTFKPILTKEARSYLVEKYKELRKDDAQGFSRSSYRITVR
QLESMIRLSEAIARANCVDEITPSFIAEAYDLLRQSIIRVDVDDVEMDEEFDNIESQS
HAASGNNDDNDDGTGSGVITSEPPADIEEGQSEATARPGTSEKKKTTVTYDKYVSMMN
MIVRKIAEVDREGAEELTAVDIVDWYLLQKENDLGSLAEYWEERRLAFKVIKRLVKDR
ILMEIHGTRHNLRDLENEENENNKTVYVIHPNCEVLDQLEPQDSS"
gene 122269..122341
/locus_tag="YNCG0005W"
/db_xref="GeneID:852674"
tRNA 122269..122341
/locus_tag="YNCG0005W"
/product="tRNA-Lys"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:8915539]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:8915539]"
/experiment="EXISTENCE:mutant phenotype:GO:0030533 triplet
codon-amino acid adaptor activity [PMID:17560369]"
/experiment="EXISTENCE:mutant phenotype:GO:0032543
mitochondrial translation [PMID:17560369]"
/note="Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE
analysis; a small portion is imported into mitochondria
via interaction with mt lysyl-tRNA synthetase Msk1p and is
necessary to decode AAG codons at high temperature, when
base modification of mt-encoded tRNA-Lys is reduced"
/db_xref="GeneID:852674"
/db_xref="SGD:S000006621"
gene complement(<122694..>123305)
/gene="EMP24"
/locus_tag="YGL200C"
/gene_synonym="BST2"
/db_xref="GeneID:852675"
mRNA complement(<122694..>123305)
/gene="EMP24"
/locus_tag="YGL200C"
/gene_synonym="BST2"
/product="Emp24p"
/transcript_id="NM_001181065.3"
/db_xref="GeneID:852675"
CDS complement(122694..123305)
/gene="EMP24"
/locus_tag="YGL200C"
/gene_synonym="BST2"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:8862519]"
/experiment="EXISTENCE:direct assay:GO:0030134
COPII-coated ER to Golgi transport vesicle
[PMID:11157978]"
/experiment="EXISTENCE:mutant phenotype:GO:0006621 protein
retention in ER lumen [PMID:8862519]"
/experiment="EXISTENCE:mutant phenotype:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:8862519]"
/experiment="EXISTENCE:mutant phenotype:GO:0016050 vesicle
organization [PMID:8862519]"
/note="Component of the p24 complex; role in misfolded
protein quality control; binds to GPI anchor proteins and
mediates their efficient transport from the ER to the
Golgi; integral membrane protein that associates with
endoplasmic reticulum-derived COPII-coated vesicles"
/codon_start=1
/product="Emp24p"
/protein_id="NP_011315.3"
/db_xref="GeneID:852675"
/db_xref="SGD:S000003168"
/translation="MASFATKFVIACFLFFSASAHNVLLPAYGRRCFFEDLSKGDELS
ISFQFGDRNPQSSSQLTGDFIIYGPERHEVLKTVRDTSHGEITLSAPYKGHFQYCFLN
ENTGIETKDVTFNIHGVVYVDLDDPNTNTLDSAVRKLSKLTREVKDEQSYIVIRERTH
RNTAESTNDRVKWWSIFQLGVVIANSLFQIYYLRRFFEVTSLV"
gene <123591..>124298
/gene="YIP4"
/locus_tag="YGL198W"
/db_xref="GeneID:852676"
mRNA <123591..>124298
/gene="YIP4"
/locus_tag="YGL198W"
/product="Yip4p"
/transcript_id="NM_001181063.1"
/db_xref="GeneID:852676"
CDS 123591..124298
/gene="YIP4"
/locus_tag="YGL198W"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:17178117]"
/experiment="EXISTENCE:physical interaction:GO:0031267
small GTPase binding [PMID:11943201]"
/note="Protein that interacts with Rab GTPases; localized
to late Golgi vesicles; computational analysis of
large-scale protein-protein interaction data suggests a
possible role in vesicle-mediated transport"
/codon_start=1
/product="Yip4p"
/protein_id="NP_011317.2"
/db_xref="GeneID:852676"
/db_xref="SGD:S000003166"
/translation="MSYGREDTTIEPDFIEPDAPLAASGGVADNIGGTMQNSGSRGTL
DETVLQTLKRDVVEINSRLKQVVYPHFPSFFSPSDDGIGAADNDISANCDLWAPLAFI
ILYSLFVSHARSLFSSLFVSSWFILLVMALHLRLTKPHQRVSLISYISISGYCLFPQV
LNALVSQILLPLAYHIGKQNRWIVRVLSLVKLVVMALCLMWSVAAVSWVTKSKTIIEI
YPLALCLFGMAWLSTIL"
gene <124698..>129161
/gene="MDS3"
/locus_tag="YGL197W"
/db_xref="GeneID:852678"
mRNA <124698..>129161
/gene="MDS3"
/locus_tag="YGL197W"
/product="Mds3p"
/transcript_id="NM_001181062.2"
/db_xref="GeneID:852678"
CDS 124698..129161
/gene="MDS3"
/locus_tag="YGL197W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:genetic interaction:GO:0075297
negative regulation of ascospore formation [PMID:9383076]"
/experiment="EXISTENCE:mutant phenotype:GO:0007124
pseudohyphal growth [PMID:24603354]"
/experiment="EXISTENCE:mutant phenotype:GO:0031929 TOR
signaling [PMID:20457806]"
/experiment="EXISTENCE:mutant phenotype:GO:0075297
negative regulation of ascospore formation [PMID:9383076]"
/note="Putative component of the TOR regulatory pathway;
negative regulator of early meiotic gene expression;
required, with Pmd1p, for growth under alkaline
conditions; has an N-terminal kelch-like domain; MDS3 has
a paralog, PMD1, that arose from the whole genome
duplication"
/codon_start=1
/product="Mds3p"
/protein_id="NP_011318.2"
/db_xref="GeneID:852678"
/db_xref="SGD:S000003165"
/translation="MPLLQPSTCFCYPLKLPPLPLTSDSNEFDECARKRLTLDYRTGS
AVTLTRSNIFVHGGLTIPLNLPVVNSMQLQKELILFFAKEKNNGSSFRNLNEWISKET
FFLDLMSRTWYRVKTSFDQRTEELLKAESSSAKADNDTNEIRTDIKKGKSLESPLKER
LFHSLCYLDGCLYIFGGLTVSPQSGYELIATNELWKLDLNTKKWSLLSDDPQIARRFN
HTMHVKNENNDNRDTKLIIVGGLNNMDQPVKKIDIYNISQNCWQSETIPKQPMEITTN
VNGIPLALSKDQNFSILVENNEANVPALAFYMRSDQIDEYLGKDSSKIKENSPIVALP
LLSESQGIRMPSNPALPKKLLNVPYELLAPTGDYFGFNIIIGGFHPNYQSSNFHCFIY
DINSGKWSRVATACPDCDINKHRFWRVFVWKSHHQTILLGTKTDDYYSPSVQRFDHLS
TFGLPLVNIFNKTIQLPHHKISASSLPIPIENFAKHKDTPLKKVSFTSSATSQFENYI
RYIAPPLEMSSIQSVFPPYAMVLGKDALEIYGKPLSDFEFITSEGDSIGIPCYLLRKR
WGRYFDMLLSQSYTKVCADYETTDTQSTLIKFSPHSSRNSSKAVRQEGRLSSSGSLDN
YFEKNFPIFARTSVSEAQNTQPQVANADAKAPNTPSTSDEPSSSSSSDLYSTPHYQRN
NDEEDDEDPVSPKPVSKSNSIYRPIRKTESSSTTSSSNGMIFRVPFKEKAAVTSNTEA
LLESNLSLQELSRRRSSLMSIPSGELLRSSISEAEHQRRASHPLTSSPLFEDSGTPCG
KQLQQLQQHTIQNPHNHLSPRRFSRSARSSISYVSSSSDRRGNSISSRSTSDSFGTPP
VLGVLNVPLPPQTREPNEPPPPCPAMSTGSNTRRSNTLTDYMHSNKASPFSSRRSSHI
GRRSSTPETENAFSATPRASLDGQMLGKSLKEGSTSQYTQPRMNSFPKANETIQTPTS
SNNEWSRQSVTSNTDSFDSLQSNFALELEPLLTPRSLYMPWPTSTVRAFAEFFYTGQV
NSKWLLAPVALDLLVMAKIYEIPLLYKLILEVLYSILAKKEESLSLICTSLMETFRTK
TLNSYKGDEEKTNTYLTSNDNYQELLKLKVSLENIDNGYYDPDLLRKQSRAQSSSTQE
SSGSANGEKTATGAGSLETSSTNVPTVFAGGPRDSHNSVGSIGFPNSMNIQGSRRSTS
GFSPRVKMKSSLSKEIDPKTFYEEYEPKEGKSFDDNDDQQTNIGSFNLHLFDMNYGSI
SSSSTNSISSSDLEEKEEQEQLQDLLEIEREDSAEILDARFRNKEDDKVTKDISNDKK
RNYLPHEKNNLKAKEGKETRDVREEEEEFDFGLGMLSLNKIKREAKHVDKVDDSVDPL
FKSSAFPQSPIRAYGSTTRTSSASGKPFRDNRSFNAFSVLTLENMASANALPPVDYVI
KSIYRTTVLVNDIRLMVRCMDCIELSKNLRALKKKTMEDISKLKGISKPSP"
gene <129883..>131169
/gene="DSD1"
/locus_tag="YGL196W"
/db_xref="GeneID:852679"
mRNA <129883..>131169
/gene="DSD1"
/locus_tag="YGL196W"
/product="D-serine ammonia-lyase DSD1"
/transcript_id="NM_001181061.1"
/db_xref="GeneID:852679"
CDS 129883..131169
/gene="DSD1"
/locus_tag="YGL196W"
/EC_number="4.3.1.18"
/experiment="EXISTENCE:direct assay:GO:0008721 D-serine
ammonia-lyase activity [PMID:17869212|PMID:17937657]"
/experiment="EXISTENCE:mutant phenotype:GO:0070178
D-serine metabolic process [PMID:17937657]"
/note="D-serine dehydratase (aka D-serine ammonia-lyase);
converts D-serine to pyruvate and ammonia by a reaction
dependent on pyridoxal 5'-phosphate and zinc; may play a
role in D-serine detoxification; L-serine is not a
substrate"
/codon_start=1
/product="D-serine ammonia-lyase DSD1"
/protein_id="NP_011319.2"
/db_xref="GeneID:852679"
/db_xref="SGD:S000003164"
/translation="MSDVLSQYKGCSVRDLPTPNFVINEEKFDKNCTTMLNNVEKLSQ
ECGVPIKFRAHVKTHKTAKGTLKQLGHGLPLAKRTTRAILVSTLKEAEELLNYQDRQC
SDYIDDITYSLPCCVPEFIPLLSNLSRRVNNFQVFVDNIEHLENLKNFGRPASGKKWS
VFIKVDMGTKRAGLAFDSPEFLSLLKKLTSSEIKEVIEPYGFYAHAGHSYSSTSINDT
QNLLMEEVKAVNSAAKVLCSVDPQFDPSKLTLSVGATPTSNSLKLDNKSTLVKFITTQ
LVSTLEIHCGNYCMYDLQQVATGCVQDHELSGFVLGTVLSSYPSRGELLSNTGVMCLT
REASSIKGFGICADLEHVLKSESFSREWYVARVSQEHGILRPIRNWNETTPLKLGSKI
AVLPQHACITMGQFPYYFVVNSEGIVNDVWLPFQKW"
gene <131525..>139543
/gene="GCN1"
/locus_tag="YGL195W"
/gene_synonym="AAS103; NDR1"
/db_xref="GeneID:852680"
mRNA <131525..>139543
/gene="GCN1"
/locus_tag="YGL195W"
/gene_synonym="AAS103; NDR1"
/product="Gcn1p"
/transcript_id="NM_001181060.3"
/db_xref="GeneID:852680"
CDS 131525..139543
/gene="GCN1"
/locus_tag="YGL195W"
/gene_synonym="AAS103; NDR1"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:9234705]"
/experiment="EXISTENCE:direct assay:GO:0022626 cytosolic
ribosome [PMID:9234705]"
/experiment="EXISTENCE:direct assay:GO:0072344 rescue of
stalled cytosolic ribosome [PMID:33338396]"
/experiment="EXISTENCE:direct assay:GO:0140469
GCN2-mediated signaling [PMID:33338396]"
/experiment="EXISTENCE:direct assay:GO:0170011 stalled
ribosome sensor activity [PMID:33338396]"
/experiment="EXISTENCE:mutant phenotype:GO:0006448
regulation of translational elongation [PMID:9234705]"
/experiment="EXISTENCE:mutant phenotype:GO:0019887 protein
kinase regulator activity [PMID:11101534]"
/note="Positive regulator of the Gcn2p kinase activity;
forms a complex with Gcn20p; proposed to stimulate Gcn2p
activation by an uncharged tRNA; suppresses frameshifting
at stalled ribosomes"
/codon_start=1
/product="Gcn1p"
/protein_id="NP_011320.3"
/db_xref="GeneID:852680"
/db_xref="SGD:S000003163"
/translation="MTAILNWEDISPVLEKGTRESHVSKRVPFLQDISQLVRQETLEK
PQLSEIAFVLLNTFTIYEDNRSKSLVTSILLDILNLEPCLLENFIRFISDVVISNPAT
KAVADYLNLLDWINSFLIFVSHNSNLFEEYIPKLLVAHSYATFGVETILDNQEEGKKS
QDKQNQHRKRIRYCIFQTTVKAFLKCLKDNDDSISFMKISIKTVLESYSKLKITSVGV
VMIMGALTQAALQLLSRQPALHSVLKENSAEKYCEYLGKEVFLGKNPPSSFCLEIGLK
PFLKEFVSQELFIKFFIPNIEKAVLRSPEVGFSILSELYAGVSPEKVNLLNAFASSKL
INQYFSSFKSSKEVVRSVSLQSMIILLRKISNTDTTLEDLTKLIDEIFKNIKSNLNAD
YKSLISKILIEIPLTHYEVSEKICKGLSPYIGKEGNEAALTLMLNAFFVHYFSLGKPI
EDLDKIISAGFADKKPALKKCWFAAFLNNSNAASEEVILNFIDGCLEFVKDSIIHYQT
HGHACILASIEFTNKILALDNTELNDRVMQLIETLPENSSIGDAILTAALSTELSIEN
RIHAVNLLQELFYKKPEFIGFSVIDAIERRMRVQELIPQQNTSFKYVTSVLLAITSEL
PDKEASIKVLINALVIAQWNIFNIKNGWAGLVLRARLDPAEVVKEHASVIMEKILEIT
GSCEWIDTIYGACGLQAAAYAAFIQPNEFTPILCKTIEADLTADDFSRLSEEDFEIFA
GEEGVLVVDVLEESMNKKLSNKNSKEYETLMWEQKIRKEQAKKNVKKLSKEEQELVNE
QLAKESAVRSHVSEISTRLKRGIRLVSELSKAACLVQNGIATWFPLAVTKLLYLCSEP
NISKLTEDVNNVFLQLSQNVSERLGNIRLFLGLATLRVHNANGISQDYLQEPLVELLT
RVLFRIKFVSNQAAIDSISLTYILPLLINVLEKGKAIALKNADKPVVKAEFVEEDEEE
EHLLLAMEIISVHAEAFEDPSIPRISIVEVLLSLLSLPSKAKIAKDCFNALCQSISVA
PNQEDLDMILSNLLSPNQFVRSTILETLDNEFELEPFMKYSPEVFICRFDSDPSNREI
ADFIWEFNKFVVNDELLKSLFPLFNQDDSGLRLFAANAYAFGAVSLFTSEENSSKDYL
NDLLNFYKEKAKPLEPILDQFGLVLVSASEQKDPWQGRSTVAITLKIMAKAFSAEDDT
VVNIIKFLVDDGGLVDREPIVRQEMKEAGVELITLHGSQNSKDLIPIFEEALSSSTDS
ALKENVIILYGTLARHLQQSDARIHTIIERLLSTLDTPSADIQQAVSACIAPLVFQFK
QKVGDYLGILMEKLLNPTVASSMRKGAAWGIAGLVKGYGISALSEFDIIRNLIEAAED
KKEPKRRESVGFCFQYLSESLGKFFEPYVIEILPNILKNLGDAVPEVRDATARATKAI
MAHTTGYGVKKLIPVAVSNLDEIAWRTKRGSVQLLGNMAYLDPTQLSASLSTIVPEIV
GVLNDSHKEVRKAADESLKRFGEVIRNPEIQKLVPVLLQAIGDPTKYTEEALDSLIQT
QFVHYIDGPSLALIIHIIHRGMHDRSANIKRKACKIVGNMAILVDTKDLIPYLQQLID
EVEIAMVDPVPNTRATAARALGALVERLGEEQFPDLIPRLLDTLSDESKSGDRLGSAQ
ALAEVISGLGLTKLDEMLPTILAGVTNFRAYIREGFMPLLLFLPVCFGSQFAPYINQI
IQPILSGLADNDENIRDTALKAGKLIVKNYATKAVDLLLPELERGMFDENDRIRLSSV
QLTGELLFQVTGISSRNEFSEEDGDHNGEFSGKLVDVLGQDRRDRILAALFVCRNDTS
GIVRATTVDIWKALVPNTPRAVKEILPTLTGMIVTHLASSSNVLRNIAAQTLGDLVRR
VGGNALSQLLPSLEESLIETSNSDSRQGVCIALYELIESASTETISQFQSTIVNIIRT
ALIDESATVREAAALSFDVFQDVVGKTAVDEVLPYLLHMLESSDNSDFALLGLQEIMS
KKSDVIFPILIPTLLAPPIDAFRASALGSLAEVAGSALYKRLSIIINALVDAIIGTSE
DESTKGALELALDRVFLSVNDDEGLHPLLQQIMSLLKSDNIEKRIAVLERLPNFFDKT
VLDFDVYIPNFVSHAILSLDDEDQRVVNGNFNALSTLLKKVDKPTLEKLVKPAKQSLA
LTGRQGQDVAAFKLPRGPNCVLPIFLHGLMYGSNDEREESALAIADVVSKTPAANLKP
FVSVITGPLIRVVGERFSSDIKAAILFALNVLFIKIPMFLRPFIPQLQRTFVKSLSDA
TNETLRLRAAKALGALIEHQPRVDPLVIELVTGAKQATDEGVKTAMLKALLEVIMKAG
SKLNENSKTNIVNLVEEEMLGSNDKLAVAYAKLIGSLSEILSNDEAHKILQDKVLNAD
LDGETGKFAILTLNSFLKDAPTHIFNTGLIDEFVSYILNAIRSPDVYFGENGTIAAGK
LLLLEGEKRSPFVKKDAAEPFKIGDENINLLINELSKAVLQPASNSTDVRRLALVVIR
TLARFKFDECIKQYFDVVGPSVFSCLRDPVIPIKLAAEKAYLALFKLVEEDDMHTFNE
WFAKISDRGNSIETVTGTTIQLRSVGDYTKRVGKRLANVERERIAAGGDAETMFSDRF
EDEREIWAVGGVELTTDI"
gene complement(<139719..>139961)
/locus_tag="YGL194C-A"
/db_xref="GeneID:3799973"
mRNA complement(<139719..>139961)
/locus_tag="YGL194C-A"
/product="uncharacterized protein"
/transcript_id="NM_001184677.1"
/db_xref="GeneID:3799973"
CDS complement(139719..139961)
/locus_tag="YGL194C-A"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/note="hypothetical protein; identified based on
comparisons of the genome sequences of six Saccharomyces
species; SWAT-GFP and mCherry fusion proteins localize to
the endoplasmic reticulum"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001032578.1"
/db_xref="GeneID:3799973"
/db_xref="SGD:S000087160"
/translation="MSNKEITCIKPFKIIALILLIVLIINLSYKLFLRRYLKSTVIWC
LGIANTDRNDMMWWQVSPLLERWVWQLVDNYESGYE"
gene complement(<140368..>141726)
/gene="HOS2"
/locus_tag="YGL194C"
/gene_synonym="RTL1"
/db_xref="GeneID:852681"
mRNA complement(<140368..>141726)
/gene="HOS2"
/locus_tag="YGL194C"
/gene_synonym="RTL1"
/product="histone deacetylase HOS2"
/transcript_id="NM_001181059.1"
/db_xref="GeneID:852681"
CDS complement(140368..141726)
/gene="HOS2"
/locus_tag="YGL194C"
/gene_synonym="RTL1"
/EC_number="3.5.1.98"
/experiment="EXISTENCE:direct assay:GO:0004407 histone
deacetylase activity [PMID:11711434]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0034399 nuclear
periphery [PMID:25817432]"
/experiment="EXISTENCE:direct assay:GO:0034967 Set3
complex [PMID:11711434]"
/experiment="EXISTENCE:direct assay:GO:0070210
Rpd3L-Expanded complex [PMID:19040720]"
/experiment="EXISTENCE:mutant phenotype:GO:0006355
regulation of DNA-templated transcription [PMID:19379692]"
/experiment="EXISTENCE:mutant phenotype:GO:0032874
positive regulation of stress-activated MAPK cascade
[PMID:18487345]"
/experiment="EXISTENCE:mutant phenotype:GO:0045727
positive regulation of translation [PMID:38783136]"
/experiment="EXISTENCE:mutant phenotype:GO:0045835
negative regulation of meiotic nuclear division
[PMID:11711434]"
/note="Histone deacetylase and subunit of Set3 and Rpd3L
complexes; required for gene activation via specific
deacetylation of lysines in H3 and H4 histone tails;
subunit of the Set3 complex, a meiotic-specific repressor
of sporulation specific genes that contains deacetylase
activity; co-localizes with Cmr1p in nuclear foci in
response to DNA damage by MMS"
/codon_start=1
/product="histone deacetylase HOS2"
/protein_id="NP_011321.1"
/db_xref="GeneID:852681"
/db_xref="SGD:S000003162"
/translation="MSGTFSYDVKTKENEPLFEFNSAYSPRVSYHFNSKVSHYHYGVK
HPMKPFRLMLTDHLVSSYGLHKIMDLYETRSATRDELLQFHSEDYVNFLSKVSPENAN
KLPRGTLENFNIGDDCPIFQNLYDYTTLYTGASLDATRKLINNQSDIAINWSGGLHHA
KKNSPSGFCYVNDIVLSILNLLRYHPRILYIDIDLHHGDGVQEAFYTTDRVFTLSFHK
YNGEFFPGTGDLTEIGCDKGKHFALNVPLEDGIDDDSYINLFKSIVDPLIMTFKPTLI
VQQCGADSLGHDRLGCFNLNIKAHGECVKFVKSFGLPMLVVGGGGYTPRNVSRLWTYE
TGILNDVLLPEDIPEDIPFRDSFGPDYSLYPMLDDLYENKNSKKLLEDIRIRCLENIR
YLQGAPSVRMDAECIPTQDISALTEEEDKIIQEMNEETEADSSNRLEEMEKENSGLIA
FS"
gene complement(141898..144120)
/gene="RME2"
/locus_tag="YNCG0006C"
/db_xref="GeneID:23547380"
ncRNA complement(141898..144120)
/ncRNA_class="antisense_RNA"
/gene="RME2"
/locus_tag="YNCG0006C"
/product="RME2"
/experiment="EXISTENCE:direct assay:GO:0010629 negative
regulation of gene expression [PMID:21300780]"
/note="Antisense transcript initiated from the 3' end of
the IME4 locus; expressed in haploids and interferes with
IME4 expression through a cis-acting mechanism; RME2
expression is repressed by the MAT a1/alpha2 repressor
complex"
/transcript_id="NR_132177.1"
/db_xref="GeneID:23547380"
/db_xref="SGD:S000144908"
gene complement(<141916..>142227)
/locus_tag="YGL193C"
/db_xref="GeneID:852682"
mRNA complement(<141916..>142227)
/locus_tag="YGL193C"
/product="uncharacterized protein"
/transcript_id="NM_001181058.1"
/db_xref="GeneID:852682"
CDS complement(141916..142227)
/locus_tag="YGL193C"
/note="Haploid-specific gene repressed by a1-alpha2;
turned off in sir3 null strains, absence enhances the
sensitivity of rad52-327 cells to campothecin almost
100-fold"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011322.1"
/db_xref="GeneID:852682"
/db_xref="SGD:S000003161"
/translation="MNSSLNANSYFFRKPPMLTYMVRFLYCYPSPFPIAPAVTDLPEC
RGDLSLSLFITSFTSTKERTILYAKSRLKTHIPVNLCDRYHYIPKAPLYQCRMPCLYS
I"
gene <142246..>144048
/gene="IME4"
/locus_tag="YGL192W"
/gene_synonym="SPO8"
/db_xref="GeneID:852683"
mRNA <142246..>144048
/gene="IME4"
/locus_tag="YGL192W"
/gene_synonym="SPO8"
/product="mRNA (N6-adenosine)-methyltransferase"
/transcript_id="NM_001181057.3"
/db_xref="GeneID:852683"
CDS 142246..144048
/gene="IME4"
/locus_tag="YGL192W"
/gene_synonym="SPO8"
/EC_number="2.1.1.348"
/experiment="EXISTENCE:direct assay:GO:0036396 RNA
N6-methyladenosine methyltransferase complex
[PMID:22685417]"
/experiment="EXISTENCE:mutant phenotype:GO:0001734 mRNA
m(6)A methyltransferase activity [PMID:12384598]"
/experiment="EXISTENCE:mutant phenotype:GO:0051321 meiotic
cell cycle [PMID:12384598|PMID:1545790|PMID:22685417]"
/experiment="EXISTENCE:mutant phenotype:GO:1902974 meiotic
DNA replication initiation [PMID:31363087]"
/experiment="EXISTENCE:mutant phenotype:GO:2000221
negative regulation of pseudohyphal growth
[PMID:22685417]"
/note="mRNA N6-adenosine methyltransferase; essential for
meiosis in S288C but dispensable for meiosis in SK1,
mediates N6-adenosine methylation of bulk mRNA during the
induction of sporulation which includes the meiotic
regulators IME1, IME2 and IME4 itself; repressed in
haploids via production of antisense IME4 transcripts;
transcribed in diploid cells where antisense transcription
is repressed; orthologous to human METTL3 (MT-A70)"
/codon_start=1
/product="mRNA (N6-adenosine)-methyltransferase"
/protein_id="NP_011323.3"
/db_xref="GeneID:852683"
/db_xref="SGD:S000003160"
/translation="MINDKLVHFLIQNYDDILRAPLSGQLKDVYSLYISGGYDDEMQK
LRNDKDEVLQFEQFWNDLQDIIFATPQSIQFDQNLLVADRPEKIVYLDVFSLKILYNK
FHAFYYTLKSSSSSCEEKVSSLTTKPEADSEKDQLLGRLLGVLNWDVNVSNQGLPREQ
LSNRLQNLLREKPSSFQLAKERAKYTTEVIEYIPICSDYSHASLLSTSVYIVNNKIVS
LQWSKISACQENHPGLIECIQSKIHFIPNIKPQTDISLGDCSYLDTCHKLNTCRYIHY
LQYIPSCLQERADRETASENKRIRSNVSIPFYTLGNCSAHCIKKALPAQWIRCDVRKF
DFRVLGKFSVVIADPAWNIHMNLPYGTCNDIELLGLPLHELQDEGIIFLWVTGRAIEL
GKESLNNWGYNVINEVSWIKTNQLGRTIVTGRTGHWLNHSKEHLLVGLKGNPKWINKH
IDVDLIVSMTRETSRKPDELYGIAERLAGTHARKLEIFGRDHNTRPGWFTIGNQLTGN
CIYEMDVERKYQEFMKSKTGTSHTGTKKIDKKQPSKLQQQHQQQYWNNMDMGSGKYYA
EAKQNPMNQKHTPFESKQQQKQQFQTLNNLYFAQ"
gene <144808..>145197
/gene="COX13"
/locus_tag="YGL191W"
/db_xref="GeneID:852684"
mRNA <144808..>145197
/gene="COX13"
/locus_tag="YGL191W"
/product="cytochrome c oxidase subunit VIa"
/transcript_id="NM_001181056.1"
/db_xref="GeneID:852684"
CDS 144808..145197
/gene="COX13"
/locus_tag="YGL191W"
/experiment="EXISTENCE:direct assay:GO:0004129
cytochrome-c oxidase activity [PMID:1331058|PMID:7851399]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24478450|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0030234 enzyme
regulator activity [PMID:10429195]"
/experiment="EXISTENCE:direct assay:GO:0045277 respiratory
chain complex IV [PMID:1331058]"
/experiment="EXISTENCE:mutant phenotype:GO:0009060 aerobic
respiration [PMID:8395517]"
/experiment="EXISTENCE:mutant phenotype:GO:0097250
mitochondrial respirasome assembly [PMID:22342701]"
/note="Subunit VIa of cytochrome c oxidase; present in a
subclass of cytochrome c oxidase complexes that may have a
role in mimimizing generation of reactive oxygen species;
not essential for cytochrome c oxidase activity but may
modulate activity in response to ATP; required for
assembly of Rcf2p into cytochrome c oxidase - cytochrome
bc1 supercomplexes"
/codon_start=1
/product="cytochrome c oxidase subunit VIa"
/protein_id="NP_011324.1"
/db_xref="GeneID:852684"
/db_xref="SGD:S000003159"
/translation="MFRQCAKRYASSLPPNALKPAFGPPDKVAAQKFKESLMATEKHA
KDTSNMWVKISVWVALPAIALTAVNTYFVEKEHAEHREHLKHVPDSEWPRDYEFMNIR
SKPFFWGDGDKTLFWNPVVNRHIEHDD"
gene complement(<145809..>147389)
/gene="CDC55"
/locus_tag="YGL190C"
/gene_synonym="TMR4"
/db_xref="GeneID:852685"
mRNA complement(<145809..>147389)
/gene="CDC55"
/locus_tag="YGL190C"
/gene_synonym="TMR4"
/product="protein phosphatase 2A regulatory subunit CDC55"
/transcript_id="NM_001181055.1"
/db_xref="GeneID:852685"
CDS complement(145809..147389)
/gene="CDC55"
/locus_tag="YGL190C"
/gene_synonym="TMR4"
/experiment="EXISTENCE:direct assay:GO:0000159 protein
phosphatase type 2A complex [PMID:8600023]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:12388751]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12388751]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:21536748]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:12388751]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:12388751]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:12388751]"
/experiment="EXISTENCE:genetic interaction:GO:0000705
achiasmate meiosis I [PMID:27455870]"
/experiment="EXISTENCE:genetic interaction:GO:0019888
protein phosphatase regulator activity [PMID:17550305]"
/experiment="EXISTENCE:genetic interaction:GO:0044818
mitotic G2/M transition checkpoint [PMID:20980617]"
/experiment="EXISTENCE:genetic interaction:GO:0045143
homologous chromosome segregation [PMID:27455870]"
/experiment="EXISTENCE:genetic interaction:GO:2000786
positive regulation of autophagosome assembly
[PMID:27973551]"
/experiment="EXISTENCE:mutant phenotype:GO:0000159 protein
phosphatase type 2A complex [PMID:8600023]"
/experiment="EXISTENCE:mutant phenotype:GO:0001100
negative regulation of exit from mitosis [PMID:16314395]"
/experiment="EXISTENCE:mutant phenotype:GO:0005634 nucleus
[PMID:23886942]"
/experiment="EXISTENCE:mutant phenotype:GO:0010971
positive regulation of G2/M transition of mitotic cell
cycle [PMID:21536748]"
/experiment="EXISTENCE:mutant phenotype:GO:0016237
microautophagy [PMID:32029270]"
/experiment="EXISTENCE:mutant phenotype:GO:0061586
positive regulation of transcription by transcription
factor localization [PMID:23275436]"
/experiment="EXISTENCE:mutant phenotype:GO:0071475
cellular hyperosmotic salinity response [PMID:23275436]"
/experiment="EXISTENCE:mutant phenotype:GO:0090266
regulation of mitotic cell cycle spindle assembly
checkpoint [PMID:23886942|PMID:27191586]"
/experiment="EXISTENCE:mutant phenotype:GO:1900182
positive regulation of protein localization to nucleus
[PMID:23275436]"
/experiment="EXISTENCE:mutant phenotype:GO:1905477
positive regulation of protein localization to membrane
[PMID:32029270]"
/note="Regulatory subunit B of protein phosphatase 2A
(PP2A); Zds1p/2p-dependent localization to cytoplasm
promotes mitotic entry; localization to nucleus prevents
mitotic exit; required for correct nuclear division,
chromosome segregation during achiasmate meiosis;
maintains nucleolar sequestration of Cdc14p in early
meiosis; limits formation of PP2A-Rts1p holocomplexes to
ensure timely dissolution of sister chromosome cohesion;
controls phosphorylation and stability of Cln2p; homolog
of mammalian B55"
/codon_start=1
/product="protein phosphatase 2A regulatory subunit CDC55"
/protein_id="NP_011325.1"
/db_xref="GeneID:852685"
/db_xref="SGD:S000003158"
/translation="MAQNNFDFKFSQCFGDKADIVVTEADLITAVEFDYTGNYLATGD
KGGRVVLFERSNSRHCEYKFLTEFQSHDAEFDYLKSLEIEEKINEIKWLRPTQRSHFL
LSTNDKTIKLWKVYEKNIKLVSQNNLTEGVTFAKKGKPDNHNSRGGSVRAVLSLQSLK
LPQLSQHDKIIAATPKRIYSNAHTYHINSISLNSDQETFLSADDLRINLWNLDIPDQS
FNIVDIKPTNMEELTEVITSAEFHPQECNLFMYSSSKGTIKLCDMRQNSLCDNKTKTF
EEYLDPINHNFFTEITSSISDIKFSPNGRYIASRDYLTVKIWDVNMDNKPLKTINIHE
QLKERLSDTYENDAIFDKFEVNFSGDSSSVMTGSYNNNFMIYPNVVTSGDNDNGIVKT
FDEHNAPNSNSNKNIHNSIQNKDSSSSGNSHKRRSNGRNTGMVGSSNSSRSSIAGGEG
ANSEDSGTEMNEIVLQADKTAFRNKRYGSLAQRSARNKDWGDDIDFKKNILHFSWHPR
ENSIAVAATNNLFIFSAL"
gene complement(<148229..>148588)
/gene="RPS26A"
/locus_tag="YGL189C"
/gene_synonym="RPS26"
/db_xref="GeneID:852686"
mRNA complement(<148229..>148588)
/gene="RPS26A"
/locus_tag="YGL189C"
/gene_synonym="RPS26"
/product="40S ribosomal protein eS26 RPS26A"
/transcript_id="NM_001181054.1"
/db_xref="GeneID:852686"
CDS complement(148229..148588)
/gene="RPS26A"
/locus_tag="YGL189C"
/gene_synonym="RPS26"
/experiment="EXISTENCE:genetic interaction:GO:0000054
ribosomal subunit export from nucleus [PMID:16246728]"
/experiment="EXISTENCE:mutant phenotype:GO:0000463
maturation of LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:25144938]"
/experiment="EXISTENCE:mutant phenotype:GO:0042255
ribosome assembly [PMID:27303706]"
/experiment="EXISTENCE:physical interaction:GO:0030686 90S
preribosome [PMID:25144938]"
/note="Protein component of the small (40S) ribosomal
subunit; homologous to mammalian ribosomal protein S26, no
bacterial homolog; RPS26A has a paralog, RPS26B, that
arose from the whole genome duplication; human homolog can
partially complement an RPS26A, RPS26B double null mutant;
mutations in the human gene are associated with
Diamond-Blackfan anemia"
/codon_start=1
/product="40S ribosomal protein eS26 RPS26A"
/protein_id="NP_011326.1"
/db_xref="GeneID:852686"
/db_xref="SGD:S000003157"
/translation="MPKKRASNGRNKKGRGHVKPVRCVNCSKSIPKDKAIKRMAIRNI
VEAAAVRDLSEASVYPEYALPKTYNKLHYCVSCAIHARIVRVRSREDRKNRAPPQRPR
FNRENKVSPADAAKKAL"
gene complement(<148824..>148964)
/locus_tag="YGL188C-A"
/db_xref="GeneID:1466453"
mRNA complement(<148824..>148964)
/locus_tag="YGL188C-A"
/product="uncharacterized protein"
/transcript_id="NM_001184593.3"
/db_xref="GeneID:1466453"
CDS complement(148824..148964)
/locus_tag="YGL188C-A"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878073.3"
/db_xref="GeneID:1466453"
/db_xref="SGD:S000028635"
/translation="MLTTQKCESREGKNDEIFELGESNSDKILLKHGKCNLFSERKPV
NH"
gene complement(<149704..>150171)
/gene="COX4"
/locus_tag="YGL187C"
/db_xref="GeneID:852688"
mRNA complement(<149704..>150171)
/gene="COX4"
/locus_tag="YGL187C"
/product="cytochrome c oxidase subunit IV"
/transcript_id="NM_001181052.1"
/db_xref="GeneID:852688"
CDS complement(149704..150171)
/gene="COX4"
/locus_tag="YGL187C"
/experiment="EXISTENCE:direct assay:GO:0004129
cytochrome-c oxidase activity [PMID:1331058|PMID:7851399]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24478450|PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0006123
mitochondrial electron transport, cytochrome c to oxygen
[PMID:1331058]"
/experiment="EXISTENCE:direct assay:GO:0008270 zinc ion
binding [PMID:17215247]"
/experiment="EXISTENCE:direct assay:GO:0045277 respiratory
chain complex IV [PMID:1331058]"
/experiment="EXISTENCE:mutant phenotype:GO:1990145
maintenance of translational fidelity [PMID:28100667]"
/note="Subunit IV of cytochrome c oxidase; the terminal
member of the mitochondrial inner membrane electron
transport chain; precursor N-terminal 25 residues are
cleaved during mitochondrial import; phosphorylated;
spermidine enhances translation"
/codon_start=1
/product="cytochrome c oxidase subunit IV"
/protein_id="NP_011328.1"
/db_xref="GeneID:852688"
/db_xref="SGD:S000003155"
/translation="MLSLRQSIRFFKPATRTLCSSRYLLQQKPVVKTAQNLAEVNGPE
TLIGPGAKEGTVPTDLDQETGLARLELLGKLEGIDVFDTKPLDSSRKGTMKDPIIIES
YDDYRYVGCTGSPAGSHTIMWLKPTVNEVARCWECGSVYKLNPVGVPNDDHHH"
gene complement(<151037..>152776)
/gene="TPN1"
/locus_tag="YGL186C"
/db_xref="GeneID:852689"
mRNA complement(<151037..>152776)
/gene="TPN1"
/locus_tag="YGL186C"
/product="Tpn1p"
/transcript_id="NM_001181051.1"
/db_xref="GeneID:852689"
CDS complement(151037..152776)
/gene="TPN1"
/locus_tag="YGL186C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:12649274]"
/experiment="EXISTENCE:direct assay:GO:0051180 vitamin
transport [PMID:12649274]"
/experiment="EXISTENCE:direct assay:GO:0090482 vitamin
transmembrane transporter activity [PMID:12649274]"
/note="Plasma membrane pyridoxine (vitamin B6)
transporter; member of the purine-cytosine permease
subfamily within the major facilitator superfamily; proton
symporter with similarity to Fcy21p, Fcy2p, and Fcy22p"
/codon_start=1
/product="Tpn1p"
/protein_id="NP_011329.1"
/db_xref="GeneID:852689"
/db_xref="SGD:S000003154"
/translation="MNRDNMDTTKRKEDHTKHTTDVIEFYEEGTAASSLNIATEKANS
SPSILRRIINRAAWLSKKVDAMGVESTGIQRISPYERGTSKKQFLHVAGLWLSATGGL
SSMSSFLLGPLLFGLSFRESVASSLISVTIGCLIAAYCSIMGPQSGCRQMVTARYLFG
WWFVKLVALASIIGVMGWSVVNSVVGGEMLAAISNDKVPLWVGIVIVTVCSFLVAIFG
IKQVIKVETYLSVPVLTAFLLLYISSSDKYSFVNAYVSKGNLDSSTRKGNWMSFFSLC
YSITATWGSITADYYILFPEDTPYIQIFCLTFFGTFLPTCFVGILGLLLASVAMSYKP
WSVEYDSHGMGGLLWAGFQRWNGFGKFCVVVLVFSLVSNNIINTYSAAFSIQLSSVFC
AKIPRWFWSIVCTIICLVCALIGRNHFSTILGNFLPMIGYWISMYFILLFEENLVFRR
FFLHLYTKEFPTVTGEINGPELVGSSKEVEKDAVTNIHLLKRKHKVTKHRYNWDKWED
YEVLTHGYAATFAFIVGVAGVVVGMAQAYWIGPIAAKFGEYGGDVAMWLSMAFSGVVY
PPCRYLELRKFGR"
gene complement(<153167..>154306)
/locus_tag="YGL185C"
/db_xref="GeneID:852690"
mRNA complement(<153167..>154306)
/locus_tag="YGL185C"
/product="putative hydroxyacid dehydrogenase"
/transcript_id="NM_001181050.1"
/db_xref="GeneID:852690"
CDS complement(153167..154306)
/locus_tag="YGL185C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0016616
oxidoreductase activity, acting on the CH-OH group of
donors, NAD or NADP as acceptor [PMID:17173333]"
/note="Putative protein with sequence similar to
hydroxyacid dehydrogenases; green fluorescent protein
(GFP)-fusion protein localizes to the cytoplasm"
/codon_start=1
/product="putative hydroxyacid dehydrogenase"
/protein_id="NP_011330.1"
/db_xref="GeneID:852690"
/db_xref="SGD:S000003153"
/translation="MCDSPATTGKPTILFIADPCETSATLNSKAFKEKFRILRYQLDT
KEAFLNFLERHEQDKICAIYAGFPAFKKIGGMTRSIIEHKSFPRKNLKCIVLCSRGYD
GWDLDTLRKHEIRLYNYQDDENEKLIDDLKLHQVGNDVADCALWHILEGFRKFSYYQK
LSRETGNTLTARAKAAEKSGFAFGHELGNMFAESPRGKKCLILGLGSIGKQVAYKLQY
GLGMEIHYCKRSEDCTMSQNESWKFHLLDETIYAKLYQFHAIVVTLPGTPQTEHLINR
KFLEHCNPGLILVNLGRGKILDLRAVSDALVTGRINHLGLDVFNKEPEIDEKIRSSDR
LTSITPHLGSATKDVFEQSCELALTRILRVVSGEAASDEHFSRVV"
gene complement(<154615..>156012)
/gene="STR3"
/locus_tag="YGL184C"
/db_xref="GeneID:852691"
mRNA complement(<154615..>156012)
/gene="STR3"
/locus_tag="YGL184C"
/product="cystathionine beta-lyase STR3"
/transcript_id="NM_001181049.3"
/db_xref="GeneID:852691"
CDS complement(154615..156012)
/gene="STR3"
/locus_tag="YGL184C"
/EC_number="4.4.1.13"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:11565790]"
/experiment="EXISTENCE:direct assay:GO:0047804
cysteine-S-conjugate beta-lyase activity
[PMID:21478306|PMID:29436228]"
/experiment="EXISTENCE:mutant phenotype:GO:0009086
methionine biosynthetic process [PMID:10821189]"
/experiment="EXISTENCE:mutant phenotype:GO:0019346
transsulfuration [PMID:10821189]"
/experiment="EXISTENCE:mutant phenotype:GO:0047804
cysteine-S-conjugate beta-lyase activity [PMID:10821189]"
/note="Peroxisomal cystathionine beta-lyase; converts
cystathionine into homocysteine; may be redox regulated by
Gto1p; involved in the release of the aromatic thiol
3-mercaptohexanol during wine fermentation"
/codon_start=1
/product="cystathionine beta-lyase STR3"
/protein_id="NP_011331.3"
/db_xref="GeneID:852691"
/db_xref="SGD:S000003152"
/translation="MPIKRLDTVVVNTGSQNDQHSASVPPVYLSTTFKVDLNNEDAQN
YDYSRSGNPTRSVLQHQIGKLYRVPQENVLAVSSGMTALDVILRGLVLLNGTDNHTPT
IIAGDDLYGGTQRLLNFFKQQSHAVSVHVDTSDFEKFKTVFQSLDKVDCVLLESPTNP
LCKVVDIPRILRFVKCISPDTTVVVDNTMMSGLNCNPLQLNPGCDVVYESATKYLNGH
HDLMGGVIISKTPEIASKLYFVINSTGAGLSPMDSWLLVRGLKTLGVRLYQQQRNAMI
LAHWLENSCGFKPTRTNKATKTRFVGLRSNPDFKLHKSFNNGPGAVLSFETGSFEHSK
RLVSSKKLSIWAVTVSFGCVNSLLSMPCKMSHASIDPELRKERDFPEDLVRLCCGIEN
IVDLKKDLLAAMVDADIIEVRENGKYLFNKLNKNLAVNTTIDDLHKPLSIYEEFYNQD
LIRKDSELNIKSSKL"
gene complement(<156543..>157285)
/gene="MND1"
/locus_tag="YGL183C"
/db_xref="GeneID:852692"
mRNA complement(join(<156543..157199,157283..>157285))
/gene="MND1"
/locus_tag="YGL183C"
/product="Mnd1p"
/transcript_id="NM_001181048.1"
/db_xref="GeneID:852692"
CDS complement(join(156543..157199,157283..157285))
/gene="MND1"
/locus_tag="YGL183C"
/experiment="EXISTENCE:direct assay:GO:0000794 condensed
nuclear chromosome [PMID:11940665]"
/experiment="EXISTENCE:direct assay:GO:0003690
double-stranded DNA binding [PMID:15249670]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:24390141]"
/experiment="EXISTENCE:mutant phenotype:GO:0007131
reciprocal meiotic recombination
[PMID:11470404|PMID:16581767]"
/note="Protein required for recombination and meiotic
nuclear division; forms a complex with Hop2p, which is
involved in chromosome pairing and repair of meiotic
double-strand breaks"
/codon_start=1
/product="Mnd1p"
/protein_id="NP_011332.2"
/db_xref="GeneID:852692"
/db_xref="SGD:S000003151"
/translation="MGPKRQTVSLQEKKNRILNFFQETYTFYNIKELEKSIPKKCGIS
PMIVKDLVQQMIDEDGVISVEKCGNINIYWCFKNQTLQKLYDSSELIKKKIQEVKCDI
ATYKQELDKTLATGRRKKFTVGQKSYNREALLEKRKKIQDEIKKKSNSLQKIESIRWD
AAKIQENKQQIRLKKVHLEKTTDNIEILIDYLYKKFFLKPEQIRKEFGIPEEFKEFTE
V"
gene <157906..>159096
/gene="GTS1"
/locus_tag="YGL181W"
/gene_synonym="FHT1; LSR1"
/db_xref="GeneID:852694"
mRNA <157906..>159096
/gene="GTS1"
/locus_tag="YGL181W"
/gene_synonym="FHT1; LSR1"
/product="Gts1p"
/transcript_id="NM_001181046.1"
/db_xref="GeneID:852694"
CDS 157906..159096
/gene="GTS1"
/locus_tag="YGL181W"
/gene_synonym="FHT1; LSR1"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:17449140|PMID:11549884]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0030479 actin
cortical patch [PMID:17449140]"
/experiment="EXISTENCE:mutant phenotype:GO:0005096 GTPase
activator activity [PMID:18208507]"
/experiment="EXISTENCE:mutant phenotype:GO:0006897
endocytosis [PMID:17449140]"
/experiment="EXISTENCE:mutant phenotype:GO:0010512
negative regulation of phosphatidylinositol biosynthetic
process [PMID:18208507]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:12782635]"
/experiment="EXISTENCE:physical interaction:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:16911513]"
/experiment="EXISTENCE:physical interaction:GO:0140297
DNA-binding transcription factor binding [PMID:16911513]"
/note="Protein involved in Arf3p regulation and in
transcription regulation; localizes to the nucleus and to
endocytic patches; contains an N-terminal Zn-finger and
ArfGAP homology domain, a C-terminal glutamine-rich
region, and a UBA (ubiquitin associated) domain; gts1
mutations affect budding, cell size, heat tolerance,
sporulation, life span, ultradian rhythms, endocytosis;
expression oscillates in a pattern similar to metabolic
oscillations"
/codon_start=1
/product="Gts1p"
/protein_id="NP_011334.1"
/db_xref="GeneID:852694"
/db_xref="SGD:S000003149"
/translation="MRFRSSSHSLKHVDRELKELINSSENANKCGECGNFYPTWCSVN
LGVFLCGRCASVHRKVFGSRDDDAFSNVKSLSMDRWTREDIDELVSLGGNKGNARFWN
PKNVPFPFDGDDDKAIVEHYIRDKYILGKFRYDEIKPEDFGSRMDDFDGESDRFDERN
RSRSRSRSHSFYKGGHNRSDYGGSRDSFQSSGSRYSRQLAELKDMGFGDTNKNLDALS
SAHGNINRAIDYLEKSSSSRNSVSAAATTSTPPLPRRRATTSGPQPAIFDGTNVITPD
FTSNSASFVQAKPAVFDGTLQQYYDPATGMIYVDQQQYAMAMQQQQQQQQQLAVAQAQ
AQAQAQAQAQVQAQAQAQAQAQAQAQQIQMQQLQMQQQQQAPLSFQQMSQGGNLPQGY
FYTQ"
gene <160065..>162758
/gene="ATG1"
/locus_tag="YGL180W"
/gene_synonym="APG1; AUT3; CVT10"
/db_xref="GeneID:852695"
mRNA <160065..>162758
/gene="ATG1"
/locus_tag="YGL180W"
/gene_synonym="APG1; AUT3; CVT10"
/product="serine/threonine protein kinase ATG1"
/transcript_id="NM_001181045.1"
/db_xref="GeneID:852695"
CDS 160065..162758
/gene="ATG1"
/locus_tag="YGL180W"
/gene_synonym="APG1; AUT3; CVT10"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0000045
autophagosome assembly [PMID:18725539]"
/experiment="EXISTENCE:direct assay:GO:0000407 phagophore
assembly site [PMID:18497569|PMID:11689437|PMID:15659643]"
/experiment="EXISTENCE:direct assay:GO:0000421
autophagosome membrane [PMID:22885598]"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0004674 protein
serine/threonine kinase activity
[PMID:33536246|PMID:9224897|PMID:22885598|PMID:25921291]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:9190802|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0006995 cellular
response to nitrogen starvation [PMID:18725539]"
/experiment="EXISTENCE:direct assay:GO:0061908 phagophore
[PMID:23549786]"
/experiment="EXISTENCE:direct assay:GO:0120095
vacuole-isolation membrane contact site [PMID:23549786]"
/experiment="EXISTENCE:direct assay:GO:1990316 Atg1/ULK1
kinase complex [PMID:26753620]"
/experiment="EXISTENCE:mutant phenotype:GO:0000045
autophagosome assembly [PMID:22778255|PMID:9224897]"
/experiment="EXISTENCE:mutant phenotype:GO:0000422
autophagy of mitochondrion [PMID:19793921]"
/experiment="EXISTENCE:mutant phenotype:GO:0004674 protein
serine/threonine kinase activity [PMID:9224897]"
/experiment="EXISTENCE:mutant phenotype:GO:0006914
autophagy [PMID:23382696]"
/experiment="EXISTENCE:mutant phenotype:GO:0006995
cellular response to nitrogen starvation [PMID:33536246]"
/experiment="EXISTENCE:mutant phenotype:GO:0016236
macroautophagy [PMID:32788210]"
/experiment="EXISTENCE:mutant phenotype:GO:0018105
peptidyl-serine phosphorylation [PMID:33536246]"
/experiment="EXISTENCE:mutant phenotype:GO:0032258
cytoplasm to vacuole targeting by the Cvt pathway
[PMID:12589048|PMID:10995454]"
/experiment="EXISTENCE:mutant phenotype:GO:0034727
piecemeal microautophagy of the nucleus [PMID:18701704]"
/experiment="EXISTENCE:mutant phenotype:GO:0044804
nucleophagy [PMID:22768199]"
/experiment="EXISTENCE:mutant phenotype:GO:0051365
cellular response to potassium ion starvation
[PMID:32788210]"
/experiment="EXISTENCE:mutant phenotype:GO:0061709
reticulophagy [PMID:26181331]"
/experiment="EXISTENCE:physical interaction:GO:1990316
Atg1/ULK1 kinase complex [PMID:15743910]"
/note="Protein serine/threonine kinase; required for
vesicle formation in autophagy and the
cytoplasm-to-vacuole targeting (Cvt) pathway; structurally
required for phagophore assembly site formation; forms a
complex with Atg13p and Atg17p during autophagy; required
for re-phosphorylation of Atg13p during termination of
autophagy, following prolonged nitrogen starvation;
essential for cell cycle progression from G2/M to G1 under
nitrogen starvation"
/codon_start=1
/product="serine/threonine protein kinase ATG1"
/protein_id="NP_011335.1"
/db_xref="GeneID:852695"
/db_xref="SGD:S000003148"
/translation="MGDIKNKDHTTSVNHNLMASAGNYTAEKEIGKGSFATVYRGHLT
SDKSQHVAIKEVSRAKLKNKKLLENLEIEIAILKKIKHPHIVGLIDCERTSTDFYLIM
EYCALGDLTFLLKRRKELMENHPLLRTVFEKYPPPSENHNGLHRAFVLSYLQQLASAL
KFLRSKNLVHRDIKPQNLLLSTPLIGYHDSKSFHELGFVGIYNLPILKIADFGFARFL
PNTSLAETLCGSPLYMAPEILNYQKYNAKADLWSVGTVVFEMCCGTPPFRASNHLELF
KKIKRANDVITFPSYCNIEPELKELICSLLTFDPAQRIGFEEFFANKVVNEDLSSYEL
EDDLPELESKSKGIVESNMFVSEYLSKQPKSPNSNLAGHQSMADNPAELSDALKNSNI
LTAPAVKTDHTQAVDKKASNNKYHNSLVSDRSFEREYVVVEKKSVEVNSLADEVAQAG
FNPNPIKHPTSTQNQNVLLNEQFSPNNQQYFQNQGENPRLLRATSSSSGGSDGSRRPS
LVDRRLSISSLNPSNALSRALGIASTRLFGGANQQQQQQQITSSPPYSQTLLNSQLFH
ELTENIILRIDHLQHPETLKLDNTNIVSILESLAAKAFVVYSYAEVKFSQIVPLSTTL
KGMANFENRRSMDSNAIAEEQDSDDAEEEDETLKKYKEDCLSTKTFGKGRTLSATSQL
SATFNKLPRSEMILLCNEAIVLYMKALSILSKSMQVTSNWWYESQEKSCSLRVNVLVQ
WLREKFNECLEKADFLRLKINDLRFKHASEVAENQTLEEKGSSEEPVYLEKLLYDRAL
EISKMAAHMELKGENLYNCELAYATSLWMLETSLDDDDFTNAYGDYPFKTNIHLKSND
VEDKEKYHSVLDENDRIIIRKYIDSIANRLKILRQKMNHQN"
rep_origin 163218..163318
/note="ARS707; Autonomously Replicating Sequence"
/db_xref="SGD:S000118453"
gene complement(<163409..>165091)
/gene="TOS3"
/locus_tag="YGL179C"
/db_xref="GeneID:852696"
mRNA complement(<163409..>165091)
/gene="TOS3"
/locus_tag="YGL179C"
/product="serine/threonine protein kinase TOS3"
/transcript_id="NM_001181044.3"
/db_xref="GeneID:852696"
CDS complement(163409..165091)
/gene="TOS3"
/locus_tag="YGL179C"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0004674 protein
serine/threonine kinase activity
[PMID:12847291|PMID:16201971]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:15879520|PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:36164978]"
/experiment="EXISTENCE:mutant phenotype:GO:0042149
cellular response to glucose starvation [PMID:15340085]"
/experiment="EXISTENCE:mutant phenotype:GO:1900180
regulation of protein localization to nucleus
[PMID:15340085]"
/note="Protein kinase; related to and functionally
redundant with Elm1p and Sak1p for the phosphorylation and
activation of Snf1p; localizes to cytosol and peroxisomes;
functionally orthologous to LKB1, a mammalian kinase
associated with Peutz-Jeghers cancer-susceptibility
syndrome; TOS3 has a paralog, SAK1, that arose from the
whole genome duplication"
/codon_start=1
/product="serine/threonine protein kinase TOS3"
/protein_id="NP_011336.3"
/db_xref="GeneID:852696"
/db_xref="SGD:S000003147"
/translation="MVLLKEPVQPLPRSSLLYNNASNSSSRIKETRKVKLLYNPLTKR
QILNNFEILATLGNGQYGKVKLARDLGTGALVAIKILNRFEKRSGYSLQLKVENPRVN
QEIEVMKRCHHENVVELYEILNDPESTKVYLVLEYCSRGPVKWCPENKMEIKAVGPSI
LTFQQSRKVVLDVVSGLEYLHSQGITHRDIKPSNLLISSNGTVKISDFGVAMSTATGS
TNIQSSHEQLLKSRALGTPAFFAPELCSTEKEYSCSSAIDIWSLGVTIYCLLFGKLPF
NANSGLELFDSIINKPLEFPSYEEMLNGATSGITMEEYTDAKDLLKKLLQKDPDKRIK
LADIKVHPFMCHYGKSDAASVLTNLETFHELKVSPPSSCKRVELVSLPVNSSFASLDS
VYMENFDHNNLRTGADRNSTYSPSIYDANTLSPSAYHNIGSRESSYSSFSSFTSSTAF
ASQISIQDAPAIGDQQCLIGESGSSLRVNSCEFPQYTTMSPVGEYPFESTEASLSSTL
TPVGNVPQRIKAHLVEGKSNSKDDLRIEADASLVFEASDAQRTRRRMSLYKL"
gene <167352..>170571
/gene="MPT5"
/locus_tag="YGL178W"
/gene_synonym="HTR1; PUF5; UTH4"
/db_xref="GeneID:852697"
mRNA join(<167352..167354,167995..>170571)
/gene="MPT5"
/locus_tag="YGL178W"
/gene_synonym="HTR1; PUF5; UTH4"
/product="Mpt5p"
/transcript_id="NM_001181043.1"
/db_xref="GeneID:852697"
CDS join(167352..167354,167995..170571)
/gene="MPT5"
/locus_tag="YGL178W"
/gene_synonym="HTR1; PUF5; UTH4"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640|PMID:11157761]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922|PMID:14562095|PMID:20498834]"
/experiment="EXISTENCE:direct assay:GO:0017148 negative
regulation of translation [PMID:20427513]"
/experiment="EXISTENCE:direct assay:GO:1900153 positive
regulation of nuclear-transcribed mRNA catabolic process,
deadenylation-dependent decay [PMID:16715093]"
/experiment="EXISTENCE:mutant phenotype:GO:0008298
intracellular mRNA localization [PMID:29297464]"
/experiment="EXISTENCE:mutant phenotype:GO:1902416
positive regulation of mRNA binding [PMID:22890846]"
/experiment="EXISTENCE:mutant phenotype:GO:1903450
regulation of G1 to G0 transition [PMID:31141453]"
/experiment="EXISTENCE:physical interaction:GO:0030674
protein-macromolecule adaptor activity
[PMID:16715093|PMID:20705650]"
/experiment="EXISTENCE:physical interaction:GO:0045947
negative regulation of translational initiation
[PMID:20705650]"
/note="mRNA-binding protein of the PUF family; binds to
specific mRNAs, often in the 3' UTR; has broad specificity
and binds to more than 1000 mRNAs (16% of the
transcriptome); recruits the CCR4-NOT deadenylase complex
to mRNAs along with Dhh1p and Dcp1p to promote
deadenylation, decapping, and decay; also interacts with
the Caf20p translational initiation repressor, affecting
its mRNA target specificity"
/codon_start=1
/product="Mpt5p"
/protein_id="NP_011337.1"
/db_xref="GeneID:852697"
/db_xref="SGD:S000003146"
/translation="MINNEPFPSADSASILTTSTSNNSLMSYNHQPQLSINSVQSLLE
PVTPPPLGQMNNKRNHQKAHSLDLSGFNQFISSTQSPLALMNNTSTSNSANSFSPNPN
AASNSTGLSASMANPPAILPLINEFDLEMDGPRRKSSHDFTVVAPSNSGVNTSSLIME
TPSSSVTPAASLRNFSNSNNAASKCGVDNSSFGLSSSTSSSMVEISALPLRDLDYIKL
ATDQFGCRFLQKKLETPSESNMVRDLMYEQIKPFFLDLILDPFGNYLVQKLCDYLTAE
QKTLLIQTIYPNVFQISINQYGTRSLQKIIDTVDNEVQIDLIIKGFSQEFTSIEQVVT
LINDLNGNHVIQKCIFKFSPSKFGFIIDAIVEQNNIITISTHKHGCCVLQKLLSVCTL
QQIFKISVKIVQFLPGLINDQFGNYIIQFLLDIKELDFYLLAELFNRLSNELCQLSCL
KFSSNVVEKFIKKLFRIITGFIVNNNGGASQRTAVASDDVINASMNILLTTIDIFTVN
LNVLIRDNFGNYALQTLLDVKNYSPLLAYNKNSNAIGQNSSSTLNYGNFCNDFSLKIG
NLIVLTKELLPSIKTTSYAKKIKLKVKAYAEATGIPFTDISPQVTAMSHNNLQTINNE
NKNPHNKNSHNHNHNHNHNHAHNNNNNNNQKSHTRHFSLPANAYHRRSNSSVTNNFSN
QYAQDQKIHSPQQIMNFNQNAYPSMGAPSFNSQTNPPLVSHNSLQNFDNRQFANLMAH
PNSAAPIHSFSSSNITNVNPNVSRGFKQPGFMMNETDKINANHFSPYSNANSQNFNES
FVPRMQYQTEGANWDSSLSMKSQHIGQGPYNQVNMSRNASISNMPAMNTARTSDELQF
TLP"
gene complement(<171415..>173079)
/locus_tag="YGL176C"
/db_xref="GeneID:852699"
mRNA complement(<171415..>173079)
/locus_tag="YGL176C"
/product="uncharacterized protein"
/transcript_id="NM_001181041.1"
/db_xref="GeneID:852699"
CDS complement(171415..173079)
/locus_tag="YGL176C"
/note="hypothetical protein; contributes to high inhibitor
stress tolerance; deletion mutant is viable"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011339.1"
/db_xref="GeneID:852699"
/db_xref="SGD:S000003144"
/translation="MTPNLPGFYYDRERRRYFRISENQSISTTGTTNQYRKDNIKRQC
VEENYDKKFSMIKKKRQQTLQKYKLSLLNPLERAFRPLSYEKYMIGLNMQYASHSLTE
GHHSHSSANVKSLNFPHRIQIGVLANCILLVTQEGCFHSKLVFATNKGYVAGFSSLDN
FSEENFFTGFSMAELNPMLKYKSEPTDVFKTMKLERTVAIKEGPSHYFYHNVNTRSNV
HTFAIFLQDFSSLKLLKIRQVKLKENCQVHDSLVVGDTLIITVNYRCHFYDLIPETFP
NPYIFSPAKSSRKHKSRSDITSLSFCLQEDALSPLKKTNTGVFYLGYRNGDSMAIVFT
NITNMTLQYSKTNGMTSESRNQPIRNSLKSVVSIKALNNKGLILISGMADKENVQQLV
IADTFLEDILTEIPVVSFKTKFLNVTKDTEILEISDDGRYFIYGSTSARDGKGDFEVF
CTTLSGNLDYEKSEGGNITLYPIGGMKNYCRLENFQFESIHLHSAFIPPRYVNPFDAV
EPLGEESSTSPYDIPEEALSQKICILIRREDDPYNGANIFITSALT"
gene complement(<173285..>174322)
/gene="SAE2"
/locus_tag="YGL175C"
/gene_synonym="COM1"
/db_xref="GeneID:852700"
mRNA complement(<173285..>174322)
/gene="SAE2"
/locus_tag="YGL175C"
/gene_synonym="COM1"
/product="ssDNA endodeoxyribonuclease SAE2"
/transcript_id="NM_001181040.1"
/db_xref="GeneID:852700"
CDS complement(173285..174322)
/gene="SAE2"
/locus_tag="YGL175C"
/gene_synonym="COM1"
/experiment="EXISTENCE:direct assay:GO:0003690
double-stranded DNA binding [PMID:18042458]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006308 DNA
catabolic process [PMID:18042458]"
/experiment="EXISTENCE:direct assay:GO:0030870 Mre11
complex [PMID:25231868]"
/experiment="EXISTENCE:direct assay:GO:0170053 nuclease
activator activity
[PMID:30819891|PMID:29321177|PMID:25231868]"
/experiment="EXISTENCE:genetic interaction:GO:0000706
meiotic DNA double-strand break processing
[PMID:20150422]"
/experiment="EXISTENCE:genetic interaction:GO:0000729 DNA
double-strand break processing [PMID:18806779]"
/experiment="EXISTENCE:genetic interaction:GO:0042138
meiotic DNA double-strand break formation [PMID:22745819]"
/experiment="EXISTENCE:mutant phenotype:GO:0000706 meiotic
DNA double-strand break processing [PMID:9215888]"
/experiment="EXISTENCE:mutant phenotype:GO:0000723
telomere maintenance [PMID:19595717]"
/experiment="EXISTENCE:mutant phenotype:GO:0000729 DNA
double-strand break processing [PMID:26490958]"
/experiment="EXISTENCE:mutant phenotype:GO:0003690
double-stranded DNA binding [PMID:18042458]"
/experiment="EXISTENCE:mutant phenotype:GO:0006308 DNA
catabolic process [PMID:18042458]"
/experiment="EXISTENCE:mutant phenotype:GO:0007534 gene
conversion at mating-type locus [PMID:18806779]"
/experiment="EXISTENCE:mutant phenotype:GO:0010791 DNA
double-strand break processing involved in repair via
synthesis-dependent strand annealing [PMID:23254329]"
/experiment="EXISTENCE:mutant phenotype:GO:0031860
telomeric 3' overhang formation [PMID:19595717]"
/experiment="EXISTENCE:mutant phenotype:GO:0170053
nuclease activator activity [PMID:18042458]"
/note="DNA-binding protein and Mre11-Rad50-Xrs2 (MRX)
complex activator; dsDNA binding protein that activates
endo- and exonuclease activities of the MRX complex to
initiate end resection during ds break repair and
processing of DNA hairpin structures; role in telomere
maintenance and formation of telomeric 3' overhangs;
solubility regulated by sumoylation and oligomeric state
by phosphorylation; removal of active Sae2p triggered by
DNA damage; sequence and functional similarity with human
CtIP/RBBP8"
/codon_start=1
/product="ssDNA endodeoxyribonuclease SAE2"
/protein_id="NP_011340.1"
/db_xref="GeneID:852700"
/db_xref="SGD:S000003143"
/translation="MVTGEENVYLKSSLSILKELSLDELLNVQYDVTTLIAKRVQALQ
NRNKCVLEEPNSKLAEILCHEKNAPQQSSQTSAGPGEQDSEDFILTQFDEDIKKESAE
VHYRNENKHTVQLPLVTMPPNRHKRKISEFSSPLNGLNNLSDLEDCSDTVIHEKDNDK
ENKTRKLLGIELENPESTSPNLYKNVKDNFLFDFNTNPLTKRAWILEDFRPNEDIAPV
KRGRRKLERFYAQVGKPEDSKHRSLSVVIESQNSDYEFAFDNLRNRSKSPPGFGRLDF
PSTQEGNEDKKKSQEIIRRKTKYRFLMASNNKIPPYEREYVFKREQLNQIVDDGCFFW
SDKLLQIYARC"
gene <174545..>175345
/gene="BUD13"
/locus_tag="YGL174W"
/gene_synonym="CWC26"
/db_xref="GeneID:852701"
mRNA <174545..>175345
/gene="BUD13"
/locus_tag="YGL174W"
/gene_synonym="CWC26"
/product="Bud13p"
/transcript_id="NM_001181039.3"
/db_xref="GeneID:852701"
CDS 174545..175345
/gene="BUD13"
/locus_tag="YGL174W"
/gene_synonym="CWC26"
/experiment="EXISTENCE:direct assay:GO:0000398 mRNA
splicing, via spliceosome [PMID:15565172]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11452010]"
/experiment="EXISTENCE:direct assay:GO:0070274 RES complex
[PMID:15565172]"
/note="Subunit of the RES complex; RES complex is required
for nuclear pre-mRNA retention and splicing; involved in
bud-site selection; diploid mutants display a unipolar
budding pattern instead of the wild-type bipolar pattern
due to a specific defect in MATa1 pre-mRNA splicing which
leads to haploid gene expression in diploids"
/codon_start=1
/product="Bud13p"
/protein_id="NP_011341.3"
/db_xref="GeneID:852701"
/db_xref="SGD:S000003142"
/translation="MALHQYLSETYGPTKPKNKTKKKKKESKSDANSDKTSLIVKERL
STLQQEQEKSGVASFSKFDKQKSKNIWKNLETNELSHAITHPSASSITGNESKNDLKE
IRAQEPLVTVADKSKTRKTIYRDAQGHKIQEDSKIDDSSFSRSKYEDEKAAEREQYLK
NLNMGDVQKLGINVDAHDKKKNQTASSLTIEDPAITFTHDKERTVKTSLLGRKLYDKP
APENRFAIMPGSRWDGVHRSNGFEEKWFAKQNEINEKKVQSYTLQEDY"
gene complement(<175527..>180113)
/gene="XRN1"
/locus_tag="YGL173C"
/gene_synonym="DST2; KEM1; RAR5; SEP1; SKI1"
/db_xref="GeneID:852702"
mRNA complement(<175527..>180113)
/gene="XRN1"
/locus_tag="YGL173C"
/gene_synonym="DST2; KEM1; RAR5; SEP1; SKI1"
/product="chromatin-binding exonuclease XRN1"
/transcript_id="NM_001181038.1"
/db_xref="GeneID:852702"
CDS complement(175527..180113)
/gene="XRN1"
/locus_tag="YGL173C"
/gene_synonym="DST2; KEM1; RAR5; SEP1; SKI1"
/experiment="EXISTENCE:direct assay:GO:0000932 P-body
[PMID:12730603|PMID:25811606]"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:23706738]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0004534 5'-3' RNA
exonuclease activity [PMID:3545079]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:23706738]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:23706738|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0016078 tRNA decay
[PMID:21632824]"
/experiment="EXISTENCE:direct assay:GO:0031370 eukaryotic
initiation factor 4G binding [PMID:30899024]"
/experiment="EXISTENCE:direct assay:GO:0090512 eisosome
membrane domain/MCC [PMID:25811606]"
/experiment="EXISTENCE:direct assay:GO:0110155 NAD-cap
decapping [PMID:35173156]"
/experiment="EXISTENCE:genetic interaction:GO:0006364 rRNA
processing [PMID:9488433]"
/experiment="EXISTENCE:genetic interaction:GO:0043144
sno(s)RNA processing [PMID:9488433]"
/experiment="EXISTENCE:mutant phenotype:GO:0000184
nuclear-transcribed mRNA catabolic process,
nonsense-mediated decay [PMID:10848586]"
/experiment="EXISTENCE:mutant phenotype:GO:0000932 P-body
[PMID:12730603]"
/experiment="EXISTENCE:mutant phenotype:GO:0000956
nuclear-transcribed mRNA catabolic process
[PMID:24119399]"
/experiment="EXISTENCE:mutant phenotype:GO:0004534 5'-3'
RNA exonuclease activity [PMID:1979303]"
/experiment="EXISTENCE:mutant phenotype:GO:0006364 rRNA
processing [PMID:9488433]"
/experiment="EXISTENCE:mutant phenotype:GO:0006413
translational initiation [PMID:30899024]"
/experiment="EXISTENCE:mutant phenotype:GO:0006995
cellular response to nitrogen starvation [PMID:29465287]"
/experiment="EXISTENCE:mutant phenotype:GO:0007089
traversing start control point of mitotic cell cycle
[PMID:16240118]"
/experiment="EXISTENCE:mutant phenotype:GO:0016242
negative regulation of macroautophagy [PMID:29465287]"
/experiment="EXISTENCE:mutant phenotype:GO:0032204
regulation of telomere maintenance [PMID:28160602]"
/experiment="EXISTENCE:mutant phenotype:GO:0032968
positive regulation of transcription elongation by RNA
polymerase II [PMID:23706738]"
/experiment="EXISTENCE:mutant phenotype:GO:0060261
positive regulation of transcription initiation by RNA
polymerase II [PMID:33258404|PMID:23706738]"
/experiment="EXISTENCE:mutant phenotype:GO:0061157 mRNA
destabilization [PMID:24567736]"
/experiment="EXISTENCE:mutant phenotype:GO:0070479
nuclear-transcribed mRNA catabolic process, 5'-3'
exonucleolytic nonsense-mediated decay [PMID:30874502]"
/experiment="EXISTENCE:mutant phenotype:GO:0070651
nonfunctional rRNA decay [PMID:19481524]"
/experiment="EXISTENCE:mutant phenotype:GO:0070966
nuclear-transcribed mRNA catabolic process, no-go decay
[PMID:31913314]"
/note="Evolutionarily-conserved 5'-3' exonuclease;
deNADing enzyme that modulates mitochondrial NAD-capped
RNA; component of cytoplasmic P-bodies involved in mRNA
decay; positively regulates transcription initiation and
elongation; involved in microtubule-mediated processes,
filamentous growth, rRNA maturation, telomere maintenance,
tRNA intron turnover; negative regulator of autophagy;
activated by glycolytic flux and scavenger decapping
enzyme Dcs1p; role in preventing L-A mycovirus
pathogenesis"
/codon_start=1
/product="chromatin-binding exonuclease XRN1"
/protein_id="NP_011342.1"
/db_xref="GeneID:852702"
/db_xref="SGD:S000003141"
/translation="MGIPKFFRYISERWPMILQLIEGTQIPEFDNLYLDMNSILHNCT
HGNDDDVTKRLTEEEVFAKICTYIDHLFQTIKPKKIFYMAIDGVAPRAKMNQQRARRF
RTAMDAEKALKKAIENGDEIPKGEPFDSNSITPGTEFMAKLTKNLQYFIHDKISNDSK
WREVQIIFSGHEVPGEGEHKIMNFIRHLKSQKDFNQNTRHCIYGLDADLIMLGLSTHG
PHFALLREEVTFGRRNSEKKSLEHQNFYLLHLSLLREYMELEFKEIADEMQFEYNFER
ILDDFILVMFVIGNDFLPNLPDLHLNKGAFPVLLQTFKEALLHTDGYINEHGKINLKR
LGVWLNYLSQFELLNFEKDDIDVEWFNKQLENISLEGERKRQRVGKKLLVKQQKKLIG
SIKPWLMEQLQEKLSPDLPDEEIPTLELPKDLDMKDHLEFLKEFAFDLGLFITHSKSK
GSYSLKMDLDSINPDETEEEFQNRVNSIRKTIKKYQNAIIVEDKEELETEKTIYNERF
ERWKHEYYHDKLKFTTDSEEKVRDLAKDYVEGLQWVLYYYYRGCPSWSWYYPHHYAPR
ISDLAKGLDQDIEFDLSKPFTPFQQLMAVLPERSKNLIPPAFRPLMYDEQSPIHDFYP
AEVQLDKNGKTADWEAVVLISFVDEKRLIEAMQPYLRKLSPEEKTRNQFGKDLIYSFN
PQVDNLYKSPLGGIFSDIEHNHCVEKEYITIPLDSSEIRYGLLPNAKLGAEMLAGFPT
LLSLPFTSSLEYNETMVFQQPSKQQSMVLQITDIYKTNNVTLEDFSKRHLNKVIYTRW
PYLRESKLVSLTDGKTIYEYQESNDKKKFGFITKPAETQDKKLFNSLKNSMLRMYAKQ
KAVKIGPMEAIATVFPVTGLVRDSDGGYIKTFSPTPDYYPLQLVVESVVNEDERYKER
GPIPIEEEFPLNSKVIFLGDYAYGGETTIDGYSSDRRLKITVEKKFLDSEPTIGKERL
QMDHQAVKYYPSYIVSKNMHLHPLFLSKITSKFMITDATGKHINVGIPVKFEARHQKV
LGYARRNPRGWEYSNLTLNLLKEYRQTFPDFFFRLSKVGNDIPVLEDLFPDTSTKDAM
NLLDGIKQWLKYVSSKFIAVSLESDSLTKTSIAAVEDHIMKYAANIEGHERKQLAKVP
REAVLNPRSSFALLRSQKFDLGDRVVYIQDSGKVPIFSKGTVVGYTTLSSSLSIQVLF
DHEIVAGNNFGGRLRTNRGLGLDASFLLNITNRQFIYHSKASKKALEKKKQSNNRNNN
TKTAHKTPSKQQSEEKLRKERAHDLLNFIKKDTNEKNSESVDNKSMGSQKDSKPAKKV
LLKRPAQKSSENVQVDLANFEKAPLDNPTVAGSIFNAVANQYSDGIGSNLNIPTPPHP
MNVVGGPIPGANDVADVGLPYNIPPGFMTHPNGLHPLHPHQMPYPNMNGMSIPPPAPH
GFGQPISFPPPPPMTNVSDQGSRIVVNEKESQDLKKFINGKQHSNGSTIGGETKNSRK
GEIKPSSGTNSTECQSPKSQSNAADRDNKKDEST"
gene <180700..>182118
/gene="NUP49"
/locus_tag="YGL172W"
/gene_synonym="NSP49"
/db_xref="GeneID:852703"
mRNA <180700..>182118
/gene="NUP49"
/locus_tag="YGL172W"
/gene_synonym="NSP49"
/product="FG-nucleoporin NUP49"
/transcript_id="NM_001181037.1"
/db_xref="GeneID:852703"
CDS 180700..182118
/gene="NUP49"
/locus_tag="YGL172W"
/gene_synonym="NSP49"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005643 nuclear
pore [PMID:10684247|PMID:1385442]"
/experiment="EXISTENCE:direct assay:GO:0017056 structural
constituent of nuclear pore [PMID:19680227]"
/experiment="EXISTENCE:direct assay:GO:0044613 nuclear
pore central transport channel
[PMID:9813081|PMID:18046406|PMID:24574455]"
/experiment="EXISTENCE:genetic interaction:GO:0017056
structural constituent of nuclear pore [PMID:11387327]"
/experiment="EXISTENCE:mutant phenotype:GO:0000055
ribosomal large subunit export from nucleus
[PMID:9971735|PMID:11071906]"
/experiment="EXISTENCE:mutant phenotype:GO:0006409 tRNA
export from nucleus [PMID:8524308]"
/experiment="EXISTENCE:mutant phenotype:GO:0006606 protein
import into nucleus [PMID:7813444]"
/experiment="EXISTENCE:mutant phenotype:GO:0016973
poly(A)+ mRNA export from nucleus [PMID:7813444]"
/experiment="EXISTENCE:physical interaction:GO:0017056
structural constituent of nuclear pore [PMID:17418788]"
/note="FG-nucleoporin component of central core of the
nuclear pore complex; contributes directly to
nucleocytoplasmic transport and maintenance of the nuclear
pore complex (NPC) permeability barrier; found in stable
complex with Nic96p and two other FG-nucleoproteins (Nsp1p
and Nup57p)"
/codon_start=1
/product="FG-nucleoporin NUP49"
/protein_id="NP_011343.1"
/db_xref="GeneID:852703"
/db_xref="SGD:S000003140"
/translation="MFGLNKASSTPAGGLFGQASGASTGNANTGFSFGGTQTGQNTGP
STGGLFGAKPAGSTGGLGASFGQQQQQSQTNAFGGSATTGGGLFGNKPNNTANTGGGL
FGANSNSNSGSLFGSNNAQTSRGLFGNNNTNNINNSSSGMNNASAGLFGSKPAGGTSL
FGNTSTSSAPAQNQGMFGAKPAGTSLFGNNAGNTTTGGGLFGSKPTGATSLFGSSNNN
NNNNNSNNIMSASGGLFGNQQQQLQQQPQMQCALQNLSQLPITPMTRISELPPQIRQE
IEQLDQYIQKQVQISHHLKADTIDHDELIDSIPRDVAYLLKSESATSQYLKQDLKKIS
SFKSLIDEDLLDTQTFSVLLQQLLTPGSKISSNDLDKFFQKKIHLYEKKLEDYCRILS
DIETAVNGIDTDLFGAPNNPNSTAITADLGSSEAENLLQLKTGLAAIVSTVIEEFTLF
MDIAERIAVLHQKTKTLASLSI"
regulatory 182286..182407
/regulatory_class="other"
/note="One of two upstream open reading frames (uORFs) in
5' untranslated region of ROK1 gene, regulate translation"
regulatory 182291..182329
/regulatory_class="other"
/note="One of two upstream open reading frames (uORFs) in
5' untranslated region of ROK1 gene, regulate translation"
gene <182390..>184084
/gene="ROK1"
/locus_tag="YGL171W"
/db_xref="GeneID:852704"
mRNA <182390..>184084
/gene="ROK1"
/locus_tag="YGL171W"
/product="RNA-dependent ATPase ROK1"
/transcript_id="NM_001181036.1"
/db_xref="GeneID:852704"
CDS 182390..184084
/gene="ROK1"
/locus_tag="YGL171W"
/EC_number="3.6.4.13"
/experiment="EXISTENCE:direct assay:GO:0003724 RNA
helicase activity [PMID:23153376]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:9154839]"
/experiment="EXISTENCE:direct assay:GO:0008186
ATP-dependent activity, acting on RNA
[PMID:18975973|PMID:23153376]"
/experiment="EXISTENCE:direct assay:GO:0030490 maturation
of SSU-rRNA [PMID:23630256]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/experiment="EXISTENCE:mutant phenotype:GO:0000447
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from
5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:9154839]"
/experiment="EXISTENCE:mutant phenotype:GO:0000472
endonucleolytic cleavage to generate mature 5'-end of
SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:9154839]"
/experiment="EXISTENCE:mutant phenotype:GO:0000480
endonucleolytic cleavage in 5'-ETS of tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:9154839]"
/experiment="EXISTENCE:mutant phenotype:GO:0048254 snoRNA
localization [PMID:18833290]"
/note="RNA-dependent ATPase; involved in pre-rRNA
processing at sites A0, A1, and A2, and in control of cell
cycle progression; contains two upstream open reading
frames (uORFs) in 5' untranslated region which regulate
translation"
/codon_start=1
/product="RNA-dependent ATPase ROK1"
/protein_id="NP_011344.1"
/db_xref="GeneID:852704"
/db_xref="SGD:S000003139"
/translation="MDIFRVLTRGASVKKESGPKAKAADYSVINGNDENHKEDNNESQ
IVKELDFFRNKRIISKVEDDREKTTENDSPNKEEKSGNDDGLIKPVITNTVEASALRK
SYKGNVSGIDIPLPIGSFEDLISRFSFDKRLLNNLIENGFTEPTPIQCECIPVALNNR
DVLACGPTGSGKTLAFLIPLVQQIIDDKQTAGLKGLIISPTKELANQIFIECFKLSYK
IFLEKKRPLQVALLSKSLGAKLKNKVVSDKKYDIIISTPLRLIDVVKNEALDLSKVKH
LIFDEADKLFDKTFVEQSDDILSACREPSLRKAMFSATIPSNVEEIAQSIMMDPVRVI
IGHKEAANTNIEQKLIFCGNEEGKLIAIRQLVQEGEFKPPIIIFLESITRAKALYHEL
MYDRINVDVIHAERTALQRDRIIERFKTGELWCLICTDVLARGIDFKGVNLVINYDVP
GSSQAYVHRIGRTGRGGRSGKAITFYTKQDSVAIKPIINVMKQSGCEVSEWMDKMAKM
TRKEKESIKNGKAHKERKQITTVPKMDKAKRRRQQEMIAASKRRKNEELSKKHFSK"
gene complement(<184153..>185394)
/gene="SPO74"
/locus_tag="YGL170C"
/db_xref="GeneID:852705"
mRNA complement(<184153..>185394)
/gene="SPO74"
/locus_tag="YGL170C"
/product="Spo74p"
/transcript_id="NM_001181035.3"
/db_xref="GeneID:852705"
CDS complement(184153..185394)
/gene="SPO74"
/locus_tag="YGL170C"
/experiment="EXISTENCE:direct assay:GO:0005198 structural
molecule activity [PMID:12796288]"
/experiment="EXISTENCE:direct assay:GO:0005816 spindle
pole body [PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0035974 meiotic
spindle pole body [PMID:12796288]"
/experiment="EXISTENCE:mutant phenotype:GO:0005198
structural molecule activity [PMID:12796288]"
/experiment="EXISTENCE:mutant phenotype:GO:0030437
ascospore formation [PMID:11470404]"
/experiment="EXISTENCE:mutant phenotype:GO:0035974 meiotic
spindle pole body [PMID:12796288]"
/note="Component of the meiotic outer plaque of the
spindle pole body; involved in modifying the meiotic outer
plaque that is required prior to prospore membrane
formation"
/codon_start=1
/product="Spo74p"
/protein_id="NP_011345.3"
/db_xref="GeneID:852705"
/db_xref="SGD:S000003138"
/translation="MGAGTLLNGLEKENFPNNIHSDLPAYPNMDSQEDGNTSKESKRN
SPVKQKSQKDEEKSSKMGTASNIFHENKDIHERSEHTDDFNDGLKLAPDSSPSLKECQ
FKNWESFWCNTEGYKTKHMQPFHFTSGLEEIKEPVMELNISTSPYKGQRPNSAPTEYS
AATTAFTKTQLEVSFLKTNLLTYIKKEIDICLSSVPFFDDAVQMQKKFLEYRDIDLDE
EYELKILGELLNDLNFFHMQENSLLNRELAVRRFSNQPESQNLPSIRDFRNPLLPIDN
RPSPPLGLKRNGKSFEETYDFTSNTSNFWGEKAELQNSITGGTPYFFHPNNIHQTKPF
MSFENQNELLFQRKNSDYKQHFNSGRNIHNGVESKSYRGVGLNDSYQKGYAAMTKSFG
NIDLNRMPRRSNEEMYSWSRN"
gene complement(185714..185786)
/locus_tag="YNCG0007C"
/db_xref="GeneID:852706"
tRNA complement(185714..185786)
/locus_tag="YNCG0007C"
/product="tRNA-Lys"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:8915539]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:8915539]"
/experiment="EXISTENCE:mutant phenotype:GO:0030533 triplet
codon-amino acid adaptor activity [PMID:17560369]"
/experiment="EXISTENCE:mutant phenotype:GO:0032543
mitochondrial translation [PMID:17560369]"
/note="Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE
analysis; a small portion is imported into mitochondria
via interaction with mt lysyl-tRNA synthetase Msk1p and is
necessary to decode AAG codons at high temperature, when
base modification of mt-encoded tRNA-Lys is reduced"
/db_xref="GeneID:852706"
/db_xref="SGD:S000006622"
gene <186059..>187339
/gene="SUA5"
/locus_tag="YGL169W"
/db_xref="GeneID:852707"
mRNA <186059..>187339
/gene="SUA5"
/locus_tag="YGL169W"
/product="threonylcarbamoyladenylate synthase"
/transcript_id="NM_001181034.1"
/db_xref="GeneID:852707"
CDS 186059..187339
/gene="SUA5"
/locus_tag="YGL169W"
/EC_number="2.7.7.87"
/experiment="EXISTENCE:direct assay:GO:0002949 tRNA
threonylcarbamoyladenosine modification [PMID:23620299]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:25038083]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0043047
single-stranded telomeric DNA binding [PMID:19369944]"
/experiment="EXISTENCE:direct assay:GO:0061710
L-threonylcarbamoyladenylate synthase activity
[PMID:23620299]"
/experiment="EXISTENCE:mutant phenotype:GO:0000723
telomere maintenance [PMID:19369944]"
/experiment="EXISTENCE:mutant phenotype:GO:0006450
regulation of translational fidelity [PMID:19884342]"
/experiment="EXISTENCE:mutant phenotype:GO:0070525 tRNA
threonylcarbamoyladenosine metabolic process
[PMID:19287007|PMID:21183954]"
/note="Protein involved in threonylcarbamoyl adenosine
biosynthesis; Sua5p and Qri7p are necessary and sufficient
for RNA t6A modification in vitro; null mutant lacks
N6-threonylcarbamoyl adenosine (t6A) modification in the
anticodon loop of ANN-decoding tRNA; member of conserved
YrdC/Sua5 family; binds single-stranded telomeric DNA and
null mutant has abnormal telomere length"
/codon_start=1
/product="threonylcarbamoyladenylate synthase"
/protein_id="NP_011346.1"
/db_xref="GeneID:852707"
/db_xref="SGD:S000003137"
/translation="MYLGRHFLAMTSKALFDTKILKVNPLSIIFSPDAHIDGSLPTIT
DPETEAALVEAARIIRDTDETVAFPTETVYGLGGSALNDNSVLSIYRAKNRPSDNPLI
THVSSIDQLNRKVFNQPHLSGTSLFDNIPSIYRPLISSLWPGPLTILLPVPSSEHSAL
SKLTTADQPTFAVRIPANPVARALIALSDTPIAAPSANASTRPSPTLASHVYHDLKDK
IPIILDGGACKVGVESTVVDGLCNPPTLLRPGGFTYEEIVKLGGEAWSLCKVENKKTV
EKGEKVRTPGMKYRHYSPSAKVVLLVPHCEGDGILKGVDRMERLKRLIETELKANSNI
KKIAILTSLKLRDSDLQSKIFNEPDFSSKTFIIERLGQSGEEIQTNLFAALRKVDEND
KVDLIFVEGINEEGEGLAVMNRLRKAAANNCIQF"
gene <187464..>187796
/gene="HUR1"
/locus_tag="YGL168W"
/db_xref="GeneID:852708"
mRNA <187464..>187796
/gene="HUR1"
/locus_tag="YGL168W"
/product="Hur1p"
/transcript_id="NM_001181033.1"
/db_xref="GeneID:852708"
CDS 187464..187796
/gene="HUR1"
/locus_tag="YGL168W"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:40704575]"
/experiment="EXISTENCE:mutant phenotype:GO:0006260 DNA
replication [PMID:12615994]"
/experiment="EXISTENCE:mutant phenotype:GO:0006303
double-strand break repair via nonhomologous end joining
[PMID:28987344]"
/experiment="EXISTENCE:mutant phenotype:GO:0016236
macroautophagy [PMID:19793921]"
/experiment="EXISTENCE:mutant phenotype:GO:0035825
homologous recombination [PMID:28987344]"
/note="Protein with a role in DNA repair; mutants show
reduced efficiency of non-homologous end-joining repair"
/codon_start=1
/product="Hur1p"
/protein_id="NP_011347.1"
/db_xref="GeneID:852708"
/db_xref="SGD:S000003136"
/translation="MFILVSVVNICTYIHLHMFPLISTFTSIGLGVLMKDKGKEGKTI
KAQNVTYQTFEKYVESSSFFFLVHNFLNSSTMKTLLLMSNNNSISEIPSFSVLKILWK
NGIYIAHI"
rep_origin 187464..187796
/gene="HUR1"
/locus_tag="YGL168W"
/note="ARS709; Replication origin; identified in multiple
array studies, confirmed by plasmid-based recombinational
ARS assay"
/db_xref="SGD:S000130163"
gene complement(<187616..>190468)
/gene="PMR1"
/locus_tag="YGL167C"
/gene_synonym="BSD1; LDB1; SSC1"
/db_xref="GeneID:852709"
mRNA complement(<187616..>190468)
/gene="PMR1"
/locus_tag="YGL167C"
/gene_synonym="BSD1; LDB1; SSC1"
/product="Ca(2+)/Mn(2+)-transporting P-type ATPase PMR1"
/transcript_id="NM_001181032.1"
/db_xref="GeneID:852709"
CDS complement(187616..190468)
/gene="PMR1"
/locus_tag="YGL167C"
/gene_synonym="BSD1; LDB1; SSC1"
/EC_number="7.2.2.10"
/experiment="EXISTENCE:direct assay:GO:0000139 Golgi
membrane [PMID:9092527|PMID:24124599]"
/experiment="EXISTENCE:direct assay:GO:0005388 P-type
calcium transporter activity [PMID:10545175]"
/experiment="EXISTENCE:direct assay:GO:0005509 calcium ion
binding [PMID:10545175]"
/experiment="EXISTENCE:direct assay:GO:0006816 calcium ion
transport [PMID:10545175]"
/experiment="EXISTENCE:direct assay:GO:0006828 manganese
ion transport [PMID:10801856]"
/experiment="EXISTENCE:direct assay:GO:0140613 P-type
manganese transporter activity [PMID:10801856]"
/experiment="EXISTENCE:genetic interaction:GO:0006874
intracellular calcium ion homeostasis [PMID:23569283]"
/experiment="EXISTENCE:genetic interaction:GO:0030026
intracellular manganese ion homeostasis [PMID:29632074]"
/experiment="EXISTENCE:mutant phenotype:GO:0005388 P-type
calcium transporter activity [PMID:10545175|PMID:1379856]"
/experiment="EXISTENCE:mutant phenotype:GO:0006816 calcium
ion transport [PMID:10545175]"
/experiment="EXISTENCE:mutant phenotype:GO:0016236
macroautophagy [PMID:19793921]"
/experiment="EXISTENCE:mutant phenotype:GO:0030026
intracellular manganese ion homeostasis [PMID:29632074]"
/note="High affinity Ca2+/Mn2+ P-type ATPase; required for
Ca2+ and Mn2+ transport into Golgi; involved in Ca2+
dependent protein sorting, processing; D53A mutant (Mn2+
transporting) is rapamycin sensitive, Q783A mutant (Ca2+
transporting) is rapamycin resistant; Mn2+ transport into
Golgi lumen required for rapamycin sensitivity; mutations
in human homolog ATP2C1 cause acantholytic skin condition
Hailey-Hailey disease; human ATP2C1 can complement yeast
null mutant"
/codon_start=1
/product="Ca(2+)/Mn(2+)-transporting P-type ATPase PMR1"
/protein_id="NP_011348.1"
/db_xref="GeneID:852709"
/db_xref="SGD:S000003135"
/translation="MSDNPFNASLLDEDSNREREILDATAEALSKPSPSLEYCTLSVD
EALEKLDTDKNGGLRSSNEANNRRSLYGPNEITVEDDESLFKKFLSNFIEDRMILLLI
GSAVVSLFMGNIDDAVSITLAIFIVVTVGFVQEYRSEKSLEALNKLVPAECHLMRCGQ
ESHVLASTLVPGDLVHFRIGDRIPADIRIIEAIDLSIDESNLTGENEPVHKTSQTIEK
SSFNDQPNSIVPISERSCIAYMGTLVKEGHGKGIVVGTGTNTSFGAVFEMMNNIEKPK
TPLQLTMDKLGKDLSLVSFIVIGMICLVGIIQGRSWLEMFQISVSLAVAAIPEGLPII
VTVTLALGVLRMAKRKAIVRRLPSVETLGSVNVICSDKTGTLTSNHMTVSKLWCLDSM
SNKLNVLSLDKNKKTKNSNGNLKNYLTEDVRETLTIGNLCNNASFSQEHAIFLGNPTD
VALLEQLANFEMPDIRNTVQKVQELPFNSKRKLMATKILNPVDNKCTVYVKGAFERIL
EYSTSYLKSKGKKTEKLTEAQKATINECANSMASEGLRVFGFAKLTLSDSSTPLTEDL
IKDLTFTGLIGMNDPPRPNVKFAIEQLLQGGVHIIMITGDSENTAVNIAKQIGIPVID
PKLSVLSGDKLDEMSDDQLANVIDHVNIFARATPEHKLNIVRALRKRGDVVAMTGDGV
NDAPALKLSDIGVSMGRIGTDVAKEASDMVLTDDDFSTILTAIEEGKGIFNNIQNFLT
FQLSTSVAALSLVALSTAFKLPNPLNAMQILWINILMDGPPAQSLGVEPVDHEVMKKP
PRKRTDKILTHDVMKRLLTTAACIIVGTVYIFVKEMAEDGKVTARDTTMTFTCFVFFD
MFNALACRHNTKSIFEIGFFTNKMFNYAVGLSLLGQMCAIYIPFFQSIFKTEKLGISD
ILLLLLISSSVFIVDELRKLWTRKKNEEDSTYFSNV"
gene <191129..>191806
/gene="CUP2"
/locus_tag="YGL166W"
/gene_synonym="ACE1"
/db_xref="GeneID:852710"
mRNA <191129..>191806
/gene="CUP2"
/locus_tag="YGL166W"
/gene_synonym="ACE1"
/product="Cup2p"
/transcript_id="NM_001181031.3"
/db_xref="GeneID:852710"
CDS 191129..191806
/gene="CUP2"
/locus_tag="YGL166W"
/gene_synonym="ACE1"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:2653812|PMID:3052856|PMID:2674688]"
/experiment="EXISTENCE:direct assay:GO:0000981 DNA-binding
transcription factor activity, RNA polymerase II-specific
[PMID:3052856]"
/experiment="EXISTENCE:direct assay:GO:0005507 copper ion
binding [PMID:2068093]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:2651899]"
/experiment="EXISTENCE:mutant phenotype:GO:0000981
DNA-binding transcription factor activity, RNA polymerase
II-specific [PMID:3052856]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:2674688]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:29604179]"
/experiment="EXISTENCE:mutant phenotype:GO:0046688
response to copper ion [PMID:3043194]"
/experiment="EXISTENCE:mutant phenotype:GO:1990169 stress
response to copper ion [PMID:29604179]"
/note="Copper-binding transcription factor; activates
transcription of the metallothionein genes CUP1-1 and
CUP1-2 in response to elevated copper concentrations;
required for regulation of copper genes in response to
DNA-damaging reagents; CUP2 has a paralog, HAA1, that
arose from the whole genome duplication"
/codon_start=1
/product="Cup2p"
/protein_id="NP_011349.3"
/db_xref="GeneID:852710"
/db_xref="SGD:S000003134"
/translation="MVVINGVKYACETCIRGHRAAQCTHTDGPLQMIRRKGRPSTTCG
HCKELRRTKNFNPSGGCMCASARRPAVGSKEDETRCRCDEGEPCKCHTKRKSSRKSKG
GSCHRRANDEAAHVNGLGIADLDVLLGLNGRSSDVDMTTTLPSLKPPLQNGEIKADSI
DNLDLASLDPLEQSPSISMEPVSINETGSAYTTTNTALNDIDIPFSINELNELYKQVS
SHNSHSQ"
gene complement(<191978..>193300)
/gene="YRB30"
/locus_tag="YGL164C"
/db_xref="GeneID:852712"
mRNA complement(<191978..>193300)
/gene="YRB30"
/locus_tag="YGL164C"
/product="Yrb30p"
/transcript_id="NM_001181029.1"
/db_xref="GeneID:852712"
CDS complement(191978..193300)
/gene="YRB30"
/locus_tag="YGL164C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:12578832]"
/experiment="EXISTENCE:direct assay:GO:0030695 GTPase
regulator activity [PMID:12578832]"
/experiment="EXISTENCE:mutant phenotype:GO:0005634 nucleus
[PMID:12578832]"
/note="RanGTP-binding protein; inhibits RanGAP1
(Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares
similarity to proteins in other fungi but not in higher
eukaryotes"
/codon_start=1
/product="Yrb30p"
/protein_id="NP_011351.1"
/db_xref="GeneID:852712"
/db_xref="SGD:S000003132"
/translation="MDEILAKAGSQAVTFAIKSGISIASTYALKTITNFVVQIPKDDA
RRIDQLKFKLESRMAIVSSAIDLIKLVAARGNTNLQITLRLTKDLKEEIDRFDEKINE
MTQKVEGSRSAKTQNEAIKAVENYIKDLLLRIEEITPFINLSLTTSGANLNSALPYQL
SPGLLLKASDFVSENNRKYEKAMKSNEKGTGDKEILKVQVGPTFEVTLFSIFYNLTSE
NNGQSGIVWKEDMKRAKARIYRLNSTGRKYDYFMKIEQDFNDGRYHEDDDKEDTPQEL
AIDLNHIKKLFFSVSGKLLRLEEQDSPVLVLKIDRSDDKENESSEGDKGLLDDITWYA
VSGYEAIEEDEEEDEEEDEEEGKDGEERKEEEEEENKLEDKDSSITLLEYIIRLTSLQ
SNDQKSILEVSDERLSIYLNDENTNSRKDRISNSTIEETEKKLKNLKL"
gene complement(<193707..>196403)
/gene="RAD54"
/locus_tag="YGL163C"
/gene_synonym="XRS1"
/db_xref="GeneID:852713"
mRNA complement(<193707..>196403)
/gene="RAD54"
/locus_tag="YGL163C"
/gene_synonym="XRS1"
/product="DNA-dependent ATPase RAD54"
/transcript_id="NM_001181028.1"
/db_xref="GeneID:852713"
CDS complement(193707..196403)
/gene="RAD54"
/locus_tag="YGL163C"
/gene_synonym="XRS1"
/EC_number="3.6.4.12"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0006338 chromatin
remodeling [PMID:12577053]"
/experiment="EXISTENCE:direct assay:GO:0015616 DNA
translocase activity [PMID:32502392|PMID:10506208]"
/experiment="EXISTENCE:direct assay:GO:0030491
heteroduplex formation [PMID:10506208]"
/experiment="EXISTENCE:direct assay:GO:0032392 DNA
geometric change [PMID:10506208]"
/experiment="EXISTENCE:mutant phenotype:GO:0000722
telomere maintenance via recombination [PMID:11238918]"
/experiment="EXISTENCE:mutant phenotype:GO:0005634 nucleus
[PMID:12399378]"
/experiment="EXISTENCE:mutant phenotype:GO:0015616 DNA
translocase activity [PMID:32502392]"
/note="DNA-dependent ATPase that stimulates strand
exchange; modifies the topology of double-stranded DNA;
drives ATP-hydrolysis-dependent sequence alignment during
homologous recombination; involved in repair of dsDNA
breaks during both mitosis and meiosis; promotes strand
invasion, catalyzes nucleosome remodeling, and removes
Rad51p from dsDNA; member of the SWI/SNF family of DNA
translocases; forms nuclear foci upon DNA replication
stress"
/codon_start=1
/product="DNA-dependent ATPase RAD54"
/protein_id="NP_011352.1"
/db_xref="GeneID:852713"
/db_xref="SGD:S000003131"
/translation="MARRRLPDRPPNGIGAGERPRLVPRPINVQDSVNRLTKPFRVPY
KNTHIPPAAGRIATGSDNIVGGRSLRKRSATVCYSGLDINADEAEYNSQDISFSQLTK
RRKDALSAQRLAKDPTRLSHIQYTLRRSFTVPIKGYVQRHSLPLTLGMKKKITPEPRP
LHDPTDEFAIVLYDPSVDGEMIVHDTSMDNKEEESKKMIKSTQEKDNINKEKNSQEER
PTQRIGRHPALMTNGVRNKPLRELLGDSENSAENKKKFASVPVVIDPKLAKILRPHQV
EGVRFLYRCVTGLVMKDYLEAEAFNTSSEDPLKSDEKALTESQKTEQNNRGAYGCIMA
DEMGLGKTLQCIALMWTLLRQGPQGKRLIDKCIIVCPSSLVNNWANELIKWLGPNTLT
PLAVDGKKSSMGGGNTTVSQAIHAWAQAQGRNIVKPVLIISYETLRRNVDQLKNCNVG
LMLADEGHRLKNGDSLTFTALDSISCPRRVILSGTPIQNDLSEYFALLSFSNPGLLGS
RAEFRKNFENPILRGRDADATDKEITKGEAQLQKLSTIVSKFIIRRTNDILAKYLPCK
YEHVIFVNLKPLQNELYNKLIKSREVKKVVKGVGGSQPLRAIGILKKLCNHPNLLNFE
DEFDDEDDLELPDDYNMPGSKARDVQTKYSAKFSILERFLHKIKTESDDKIVLISNYT
QTLDLIEKMCRYKHYSAVRLDGTMSINKRQKLVDRFNDPEGQEFIFLLSSKAGGCGIN
LIGANRLILMDPDWNPAADQQALARVWRDGQKKDCFIYRFISTGTIEEKIFQRQSMKM
SLSSCVVDAKEDVERLFSSDNLRQLFQKNENTICETHETYHCKRCNAQGKQLKRAPAM
LYGDATTWNHLNHDALEKTNDHLLKNEHHYNDISFAFQYISH"
gene <198138..>199037
/gene="SUT1"
/locus_tag="YGL162W"
/db_xref="GeneID:852714"
mRNA <198138..>199037
/gene="SUT1"
/locus_tag="YGL162W"
/product="Sut1p"
/transcript_id="NM_001181027.1"
/db_xref="GeneID:852714"
CDS 198138..199037
/gene="SUT1"
/locus_tag="YGL162W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11248676|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:genetic interaction:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:23872066]"
/experiment="EXISTENCE:genetic interaction:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:11401714]"
/experiment="EXISTENCE:genetic interaction:GO:1900237
positive regulation of induction of conjugation with
cellular fusion [PMID:23872066]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:23223039]"
/experiment="EXISTENCE:mutant phenotype:GO:0009267
cellular response to starvation [PMID:23223039]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:19793923]"
/experiment="EXISTENCE:mutant phenotype:GO:1900429
negative regulation of filamentous growth of a population
of unicellular organisms [PMID:23223039]"
/experiment="EXISTENCE:mutant phenotype:GO:2000911
positive regulation of sterol import [PMID:19793923]"
/experiment="EXISTENCE:physical interaction:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:11401714]"
/note="Zn(II)2Cys6 family transcription factor; positively
regulates sterol uptake genes under anaerobic conditions;
involved in hypoxic gene expression; represses
filamentation-inducing genes during vegetative growth;
positively regulates mating with SUT2 by repressing
expression of genes that act as mating inhibitors;
repressed by STE12; relocalizes from the nucleus to the
cytoplasm upon DNA replication stress; SUT1 has a paralog,
SUT2, that arose from the whole genome duplication"
/codon_start=1
/product="Sut1p"
/protein_id="NP_011353.1"
/db_xref="GeneID:852714"
/db_xref="SGD:S000003130"
/translation="MSTSITVRNRDRSLPPLLLPNVSLLEKDIRRKGTQNVGITDPEL
LSTTWTRKRAFPTDELLGGYKRLKPAAADSNECAIGIATVTPPPTLPVSAIVPPPQNY
TPPLFEYHPHALASMVNEDANASCTQMSIISRSTSNSTTSSATSTSSISKRQRSGPSC
DKCRLKKIKCNAKIEILLQDDTIMPMISNKLRYVLTPDDIRLYRGTLLRNIAIPDDVI
EGTGSRKLIKHIDKLVLLTPCLPCIKKKHSSSSTNFPKNDKCTFSKGFTRADINISSK
ISLKFKDKTIYDITYDDYKSIDF"
gene complement(<199210..>200142)
/gene="YIP5"
/locus_tag="YGL161C"
/db_xref="GeneID:852715"
mRNA complement(<199210..>200142)
/gene="YIP5"
/locus_tag="YGL161C"
/product="Yip5p"
/transcript_id="NM_001181026.3"
/db_xref="GeneID:852715"
CDS complement(199210..200142)
/gene="YIP5"
/locus_tag="YGL161C"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:17178117]"
/experiment="EXISTENCE:physical interaction:GO:0031267
small GTPase binding [PMID:11943201]"
/note="Protein that interacts with Rab GTPases; localized
to late Golgi vesicles; computational analysis of
large-scale protein-protein interaction data suggests a
possible role in vesicle-mediated transport"
/codon_start=1
/product="Yip5p"
/protein_id="NP_011354.3"
/db_xref="GeneID:852715"
/db_xref="SGD:S000003129"
/translation="MPSNNSSFLDIDDDLEGVDDFGNEPNPFDDATVPDSPNMNNSTA
GKGSEFYNTTGSKAESAPLQGQMDPPAYDQVIGQNDNDGLGRNGLRPGLINYYSKYFQ
IDLTQFKKRLSAVLTFRNDHNSESNEDNTDLYGAVWITATVVMINFTMSRGLNFIISD
VIEGVKTGEDIDRASQFKKLLHSIWLFYGYTFGVPFITMQVLNRDEHSERNRSFKSVP
ELISVYGYANLIWIPVCVILNILDMSKRLRTVQAIQWAIVALGWAQSSYFLNSQISSN
NNTETQSNGKFSLSIIVVVALHTLFCLLFRFIIF"
gene <200561..>202273
/gene="AIM14"
/locus_tag="YGL160W"
/gene_synonym="YNO1"
/db_xref="GeneID:852716"
mRNA <200561..>202273
/gene="AIM14"
/locus_tag="YGL160W"
/gene_synonym="YNO1"
/product="putative metalloreductase"
/transcript_id="NM_001181025.1"
/db_xref="GeneID:852716"
CDS 200561..202273
/gene="AIM14"
/locus_tag="YGL160W"
/gene_synonym="YNO1"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0097038 perinuclear
endoplasmic reticulum [PMID:22586098]"
/experiment="EXISTENCE:genetic interaction:GO:0006915
apoptotic process [PMID:22586098]"
/experiment="EXISTENCE:mutant phenotype:GO:0016175
superoxide-generating NAD(P)H oxidase activity
[PMID:22586098]"
/experiment="EXISTENCE:mutant phenotype:GO:0032956
regulation of actin cytoskeleton organization
[PMID:22586098]"
/note="NADPH oxidase localized to the perinuclear ER;
produces superoxide from NADPH; overexpression causes MCA1
dependent apoptosis; likely involved in
superoxide-mediated regulation of the actin cytoskeleton;
member of a conserved superfamily of NADPH oxidases (NOX
enzymes); has similarity to iron/copper reductases
(FRE1-8), particularly Fre8p"
/codon_start=1
/product="putative metalloreductase"
/protein_id="NP_011355.1"
/db_xref="GeneID:852716"
/db_xref="SGD:S000003128"
/translation="MKESPLITLVKRHSETHFANIKYGYYVLIISLVYLIGLALLRAF
GRRTPSRSSSAFKNKIIYRLYDIDPAIHLGILFFAVLIPFYYHYSLTTQSTVYLKRLG
RLSYALIPLNLFLTLRPNWFLRKNCTYTDFIPFHKWFSRIITVIGLLHGIFFIIKWAI
DDNVSLKQKLILKTFNFAGFIISILVLFLLICSIGPMRRYNYRLFYIVHNLVNVAFIL
LTPIHSRPGVKFPFLLLNCTLLFIHIINRIVFAKSLMILNKNANYSKTNLVHVRLPRA
ILPDYFEPGSHIRISPYRRINPLYWLLPSHPYTIASLAEDNSIDLIIKETSTAEPGSQ
IESLRSNPKSFHLDQEKTYTLINSYPPSVPEECYSQGTNIAIICGGSGISFALPLFRH
FFNKENVKYLKMIWLIKDYSEYELVLDYLKTNGLTFEKKLSNNKRISVFISGEYTAET
RLDEITTNIDDENSEYEMGSFNNEDEDLSISNFNSENADSNDNTPETSHSPTKENGSM
IEVKSKHSFTLSNELKSFNNESAQVNQNETWLFSCGPPSLLQLSKKYCNDERINFVCE
TYGL"
gene <202721..>203833
/locus_tag="YGL159W"
/db_xref="GeneID:852717"
mRNA <202721..>203833
/locus_tag="YGL159W"
/product="uncharacterized protein"
/transcript_id="NM_001181024.1"
/db_xref="GeneID:852717"
CDS 202721..203833
/locus_tag="YGL159W"
/note="hypothetical protein; deletion mutant has no
detectable phenotype"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011356.1"
/db_xref="GeneID:852717"
/db_xref="SGD:S000003127"
/translation="MNNSIITDDEVREFYLNCSSQTIIESLLSLHESLRLYSQNHEIL
LNRMFKKLDETNADSNISHIFMPVVSKDFSGIKILVNNNNKNFQGVINVIEPETGKLI
GCFEAKQITAIRTALASCIGLYKQLSCSHDKLFRFENGTCYLTCFGTGLQAFWHIYIA
IKLIMSGIVGESLKLVEINILYHNNMMSLDRLKSLKNLFGSNIKIELNQYQINDISSE
GNGAVSNSDIIFGCLPTLEPNLFLRQLLNSKASVEQKHTYISLIGSYKPVMHECDKEL
IDKFKSDNESACILVDSREHTLLESGELIDSNIAPHNLIEIGELDTLKNTVLNLNEKG
CKRTITLCKIVGLAVMDVALAKEFLSLRTKNTENKE"
rep_origin 203914..204155
/note="ARS710; Autonomously Replicating Sequence"
/db_xref="SGD:S000118454"
repeat_region 204982..205337
/note="Ty4 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006951"
gene 205521..205634
/locus_tag="YNCG0008W"
/db_xref="GeneID:852718"
tRNA join(205521..205558,205591..205634)
/locus_tag="YNCG0008W"
/product="tRNA-Leu"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:852718"
/db_xref="SGD:S000006639"
gene <207033..>208571
/gene="RCK1"
/locus_tag="YGL158W"
/db_xref="GeneID:852719"
mRNA <207033..>208571
/gene="RCK1"
/locus_tag="YGL158W"
/product="putative serine/threonine protein kinase RCK1"
/transcript_id="NM_001181023.1"
/db_xref="GeneID:852719"
CDS 207033..208571
/gene="RCK1"
/locus_tag="YGL158W"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:genetic interaction:GO:0034599
cellular response to oxidative stress [PMID:15341652]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress
[PMID:39326682|PMID:15341652]"
/experiment="EXISTENCE:mutant phenotype:GO:0040020
regulation of meiotic nuclear division [PMID:10778743]"
/note="Protein kinase involved in oxidative stress
response; promotes pseudohyphal growth via activation of
Ubp3p phosphorylation; identified as suppressor of S.
pombe cell cycle checkpoint mutations; RCK1 has a paralog,
RCK2, that arose from the whole genome duplication"
/codon_start=1
/product="putative serine/threonine protein kinase RCK1"
/protein_id="NP_011357.1"
/db_xref="GeneID:852719"
/db_xref="SGD:S000003126"
/translation="MSVNPEFIADGIDFYPTTPDAAYFNAADGKNKVNRINGNSENLH
HSFASGCRRSSLSVDFNVTSSDSEKSEQSCLENNSQEDEYFCDIFSTELKLDETSNKS
TDYSSSNHQYPEQLELHNYKLLNKIGEGAFSRVFKAVGINTDDQAPVAIKAIIKKGIS
SDAILKGNDRIQGSSRKKVLNEVAIHKLVSKNNPHCTKFIAFQESANYYYLVTELVTG
GEIFDRIVQLTCFSEDLARHVITQVAIAIKHMHYMGIVHRDVKPENLLFEPIPFYGLD
GDMQKEDEFTLGVGGGGIGLVKLMDFGLAKKLRNNTAKTPCGTIEYVASEVFTSKRYS
MKVDMWSIGCVLFTLLCGYPPFYEKNEKTLLKKISRGDYEFLAPWWDNISSGAKNAVT
HLLEVDPNKRYDIDDFLNDPWLNSYDCLKDSNSNSYASVQSILNDSFDERAETLHCAL
SCQSEKQDDTEFSRSESSEYIFMTEEDRNLRGSWIGEPKECFTLDLATSSIYRRRKNK
IFFW"
gene <209006..>210049
/gene="ARI1"
/locus_tag="YGL157W"
/db_xref="GeneID:852720"
mRNA <209006..>210049
/gene="ARI1"
/locus_tag="YGL157W"
/product="carbonyl reductase (NADPH-dependent) ARI1"
/transcript_id="NM_001181022.3"
/db_xref="GeneID:852720"
CDS 209006..210049
/gene="ARI1"
/locus_tag="YGL157W"
/experiment="EXISTENCE:direct assay:GO:0004090 carbonyl
reductase (NADPH) activity [PMID:19016485|PMID:19577617]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0016491
oxidoreductase activity [PMID:14574691]"
/note="NADPH-dependent aldehyde reductase; utilizes
aromatic and alophatic aldehyde substrates; member of the
short-chain dehydrogenase/reductase superfamily"
/codon_start=1
/product="carbonyl reductase (NADPH-dependent) ARI1"
/protein_id="NP_011358.3"
/db_xref="GeneID:852720"
/db_xref="SGD:S000003125"
/translation="MTTDTTVFVSGATGFIALHIMNDLLKAGYTVIGSGRSQEKNDGL
LKKFNNNPKLSMEIVEDIAAPNAFDEVFKKHGKEIKIVLHTASPFHFETTNFEKDLLT
PAVNGTKSILEAIKKYAADTVEKVIVTSSTAALVTPTDMNKGDLVITEESWNKDTWDS
CQANAVAAYCGSKKFAEKTAWEFLKENKSSVKFTLSTINPGFVFGPQMFADSLKHGIN
TSSGIVSELIHSKVGGEFYNYCGPFIDVRDVSKAHLVAIEKPECTGQRLVLSEGLFCC
QEIVDILNEEFPQLKGKIATGEPATGPSFLEKNSCKFDNSKTKKLLGFQFYNLKDCIV
DTAAQMLEVQNEA"
gene <210416..>213667
/gene="AMS1"
/locus_tag="YGL156W"
/db_xref="GeneID:852721"
mRNA <210416..>213667
/gene="AMS1"
/locus_tag="YGL156W"
/product="alpha-mannosidase"
/transcript_id="NM_001181021.1"
/db_xref="GeneID:852721"
CDS 210416..213667
/gene="AMS1"
/locus_tag="YGL156W"
/EC_number="3.2.1.24"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:3281936]"
/experiment="EXISTENCE:direct assay:GO:0004559
alpha-mannosidase activity [PMID:3281936]"
/experiment="EXISTENCE:direct assay:GO:0034270 Cvt complex
[PMID:12479808|PMID:20659891]"
/experiment="EXISTENCE:mutant phenotype:GO:0006995
cellular response to nitrogen starvation [PMID:26947009]"
/experiment="EXISTENCE:mutant phenotype:GO:0009313
oligosaccharide catabolic process [PMID:12723970]"
/experiment="EXISTENCE:mutant phenotype:GO:0019309 mannose
catabolic process [PMID:26947009]"
/experiment="EXISTENCE:mutant phenotype:GO:0042149
cellular response to glucose starvation [PMID:26947009]"
/note="Vacuolar alpha mannosidase; involved in free
oligosaccharide (fOS) degradation; upregulated under
glucose starvation; targeted to vacuole via CVT pathway
and AP-3 pathway"
/codon_start=1
/product="alpha-mannosidase"
/protein_id="NP_011359.1"
/db_xref="GeneID:852721"
/db_xref="SGD:S000003124"
/translation="MSSEDIIYDPQFKPVQGIYENRLRQFIDTGGDYHDLNLPKFYDK
KRISLDHDHVKVWWYQVSFERGSSPVSPDKRPSWKSIIERDKKGELEFREANINQPFG
PSWSTTWFKVKISLPEDWVKSNEQLLFQWDCSNEGIVIDPKTLIPVTAFSGGERTEYV
LPKTSDGKHFFYIEAGNNGMFGCGAGSTINPPDDNRFFHLRKADIVWPDLDARALYID
FWMLGDAARELPGDSWQKHQARQLGNAVMNLFDPNDRSSVRKCRELLQREYFDSFLES
SKVYEQGESQVLTNVYGIGNCHIDTAWLWPFAETRRKIVRSWSSQCTLMDRFPEYKFV
ASQAQQFKWLLEDHPEFFNKVLIPKIQQSQFFAVGGTWVENDTNIPSGESLARQFFFG
QRFFLKHFGLKSKIFWLPDTFGYSSQMPQLCRLSGIDKFLTQKLSWNNINSFPHSTFN
WAGIDGSQLLTHMPPGNTYTADSHFGDVLRTAKQNKTPEYYGSGLMLYGKGDGGGGPT
EEMLQKMRRIRSMNNRNGNVIPKLQVGITVDEFYDDILKRTNQGHDLPTWSGELYFEF
HRGTYTSQAQTKKLMRLSEIKLHDLEWIAAKTSVLYPDSYKYPSKQINELWENVLLCQ
FHDVLPGSCIEMVYKYEAVPMLHNVVKECTSLIDKTVQFLQSQSKADLVEMRTLTWSK
PEKVSEECSLNGSYTSSVTGYDDYIVLANGKLKVIICKKTGVITSITDETLGVEYLDT
EHGRNKLGANQFVIYDDKPLGWQAWDTELYSVNQYKYVTKPKKVQVSCNTKEKCAVEV
IFQISEKCKIKSVISLNATAVTDAKLSKVDISTTVENWDARNKFLKVEFPVNIRNDFA
SYETQFGITKRPTHYNTSWDVAKFEVCHHKFADYSEYSKGVSILNDCKYGFSTHGNLM
RLSLLRSPKAPDAHADMGTHEIKYAIYPHRGALSSDTVKLAHEFNYCFKYKLPKDIGM
NFDDIISISGDENVILSNIKRGEDDSAVKSNYSLNPRDEQSIVVRVYESLGGESFASL
NTTLNLKRIEKVDNLEMKVYKSLTATRDESNHAINRIPIKLRPFEIASFRLYF"
gene <214081..>215211
/gene="CDC43"
/locus_tag="YGL155W"
/gene_synonym="CAL1"
/db_xref="GeneID:852722"
mRNA <214081..>215211
/gene="CDC43"
/locus_tag="YGL155W"
/gene_synonym="CAL1"
/product="protein geranylgeranyltransferase type I subunit
CDC43"
/transcript_id="NM_001181020.1"
/db_xref="GeneID:852722"
CDS 214081..215211
/gene="CDC43"
/locus_tag="YGL155W"
/gene_synonym="CAL1"
/EC_number="2.5.1.59"
/experiment="EXISTENCE:direct assay:GO:0004662
CAAX-protein geranylgeranyltransferase activity
[PMID:1400380]"
/experiment="EXISTENCE:direct assay:GO:0005953
CAAX-protein geranylgeranyltransferase complex
[PMID:1400380]"
/experiment="EXISTENCE:direct assay:GO:0018344 protein
geranylgeranylation [PMID:2034682]"
/note="Beta subunit of geranylgeranyltransferase type I;
subunit of the Ram2p-Cdc43p heterodimer that catalyzes the
geranylgeranylation of the cysteine residue in proteins
containing a C-terminal CaaX sequence ending in Leu or
Phe; has substrates important for morphogenesis"
/codon_start=1
/product="protein geranylgeranyltransferase type I subunit
CDC43"
/protein_id="NP_011360.1"
/db_xref="GeneID:852722"
/db_xref="SGD:S000003123"
/translation="MCQATNGPSRVVTKKHRKFFERHLQLLPSSHQGHDVNRMAIIFY
SISGLSIFDVNVSAKYGDHLGWMRKHYIKTVLDDTENTVISGFVGSLVMNIPHATTIN
LPNTLFALLSMIMLRDYEYFETILDKRSLARFVSKCQRPDRGSFVSCLDYKTNCGSSV
DSDDLRFCYIAVAILYICGCRSKEDFDEYIDTEKLLGYIMSQQCYNGAFGAHNEPHSG
YTSCALSTLALLSSLEKLSDKFKEDTITWLLHRQVSSHGCMKFESELNASYDQSDDGG
FQGRENKFADTCYAFWCLNSLHLLTKDWKMLCQTELVTNYLLDRTQKTLTGGFSKNDE
EDADLYHSCLGSAALALIEGKFNGELCIPQEIFNDFSKRCCF"
gene complement(<215278..>216096)
/gene="LYS5"
/locus_tag="YGL154C"
/db_xref="GeneID:852723"
mRNA complement(<215278..>216096)
/gene="LYS5"
/locus_tag="YGL154C"
/product="holo-[acyl-carrier-protein] synthase"
/transcript_id="NM_001181019.1"
/db_xref="GeneID:852723"
CDS complement(215278..216096)
/gene="LYS5"
/locus_tag="YGL154C"
/EC_number="2.7.8.7"
/experiment="EXISTENCE:direct assay:GO:0008897
holo-[acyl-carrier-protein] synthase activity
[PMID:10320345]"
/experiment="EXISTENCE:mutant phenotype:GO:0019878
L-lysine biosynthetic process via aminoadipic acid
[PMID:3921525]"
/note="Phosphopantetheinyl transferase involved in lysine
biosynthesis; converts inactive apo-form of Lys2p
(alpha-aminoadipate reductase) into catalytically active
holo-form by posttranslational addition of
phosphopantetheine"
/codon_start=1
/product="holo-[acyl-carrier-protein] synthase"
/protein_id="NP_011361.1"
/db_xref="GeneID:852723"
/db_xref="SGD:S000003122"
/translation="MVKTTEVVSEVSKVAGVRPWAGIFVVEIQEDILADEFTFEALMR
TLPLASQARILNKKSFHDRCSNLCSQLLQLFGCSIVTGLNFQELKFDKGSFGKPFLDN
NRFLPFSMTIGEQYVAMFLVKCVSTDEYQDVGIDIASPCNYGGREELELFKEVFSERE
FNGLLKASDPCTIFTYLWSLKESYTKFTGTGLNTDLSLIDFGAISFFPAEGASMCITL
DEVPLIFHSQWFNNEIVTICMPKSISDKINTNRPKLYNISLSTLIDYFIENDGL"
gene <216273..>217298
/gene="PEX14"
/locus_tag="YGL153W"
/db_xref="GeneID:852724"
mRNA <216273..>217298
/gene="PEX14"
/locus_tag="YGL153W"
/product="Pex14p"
/transcript_id="NM_001181018.3"
/db_xref="GeneID:852724"
CDS 216273..217298
/gene="PEX14"
/locus_tag="YGL153W"
/experiment="EXISTENCE:direct assay:GO:0005778 peroxisomal
membrane [PMID:9094717|PMID:9312008]"
/experiment="EXISTENCE:direct assay:GO:0008320 protein
transmembrane transporter activity [PMID:20154681]"
/experiment="EXISTENCE:direct assay:GO:0016560 protein
import into peroxisome matrix, docking [PMID:9094717]"
/experiment="EXISTENCE:direct assay:GO:0016561 protein
import into peroxisome matrix, translocation
[PMID:20154681]"
/experiment="EXISTENCE:direct assay:GO:0030674
protein-macromolecule adaptor activity [PMID:9312008]"
/experiment="EXISTENCE:direct assay:GO:1990429 peroxisomal
importomer complex
[PMID:20154681|PMID:12667447|PMID:22375831]"
/experiment="EXISTENCE:mutant phenotype:GO:0016560 protein
import into peroxisome matrix, docking [PMID:16107331]"
/experiment="EXISTENCE:physical interaction:GO:0016560
protein import into peroxisome matrix, docking
[PMID:9312008|PMID:9094717|PMID:16107331]"
/experiment="EXISTENCE:physical interaction:GO:0030674
protein-macromolecule adaptor activity
[PMID:9312008|PMID:9094717]"
/note="Central component of the peroxisomal importomer
complex; peroxisomal protein import machinery docking
complex component; interacts with both PTS1 (Pex5p) and
PTS2 (Pex7p) peroxisomal matrix protein signal recognition
factors and membrane receptor Pex13p"
/codon_start=1
/product="Pex14p"
/protein_id="NP_011362.3"
/db_xref="GeneID:852724"
/db_xref="SGD:S000003121"
/translation="MSDVVSKDRKALFDSAVSFLKDESIKDAPLLKKIEFLKSKGLTE
KEIEIAMKEPKKDGIVGDEVSKKIGSTENRASQDMYLYEAMPPTLPHRDWKDYFVMAT
ATAGLLYGAYEVTRRYVIPNILPEAKSKLEGDKKEIDDQFSKIDTVLNAIEAEQAEFR
KKESETLKELSDTIAELKQALVQTTRSREKIEDEFRIVKLEVVNMQNTIDKFVSDNDG
MQELNNIQKEMESLKSLMNNRMESGNAQDNRLFSISPNGIPGIDTIPSASEILAKMGM
QEESDKEKENGSDANKDDNAVPAWKKAREQTIDSNASIPEWQKNTAANEISVPDWQNG
QVEDSIP"
gene <217524..>220922
/gene="NUT1"
/locus_tag="YGL151W"
/gene_synonym="MED5; SSX4"
/db_xref="GeneID:852726"
mRNA <217524..>220922
/gene="NUT1"
/locus_tag="YGL151W"
/gene_synonym="MED5; SSX4"
/product="Nut1p"
/transcript_id="NM_001181016.1"
/db_xref="GeneID:852726"
CDS 217524..220922
/gene="NUT1"
/locus_tag="YGL151W"
/gene_synonym="MED5; SSX4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9671481|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0006366
transcription by RNA polymerase II [PMID:19368889]"
/experiment="EXISTENCE:direct assay:GO:0016592 mediator
complex [PMID:19368889]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:9671481]"
/note="Component of the RNA polymerase II mediator
complex; mediator is required for transcriptional
activation and also has a role in basal transcription"
/codon_start=1
/product="Nut1p"
/protein_id="NP_011364.1"
/db_xref="GeneID:852726"
/db_xref="SGD:S000003119"
/translation="MEKESVYNLALKCAERQLTSMEFSNLYKEFFNEKFPSLIQEEEE
DTTTTANINEVKKASDLVDTPSNNTAATADTTHLHEALDIVCSDFVKILNLEKPLILA
DYIVEVLLVNYNSDMIKCFLPKLNSVRNSLLLAHFFSKSCSFFAKLSDTLIIDQVRKD
LGNVIVPNILSLDMNSMNKELIAIVSKLLQTTLKLSPSPILLTSAGCKNGSFTLLNQL
SQTNKLLFKRVSQTFEAKLHFKDTKPFLNKDSTNEFVGSPSLTSPQYIPSPLSSTKPP
GSVNSAAKYKDMKLLRYYKNIWLNNKIINWEISNPDFLSKYSAITSSIFQESFNSVQN
LDQLLTDLIETSFTCFAQFVSNKQYHQANSNLTLLERKWVIFITKHLPLLILENSSRS
PRVVTNALDNIDEKVVKAIRIYFTEKDDNKTNNEDLFDDYPSTSLDIRHDFIKGLIML
NLQPASVINNYLREDQMIDTSILPTRDDLFVRNLQGIQEVVHNTNSFIISSLDTLELE
SITESITHDSSNGLFQVLHNFESVAPTKQREIVKAFLSIFEDAIKELNYNRIAKICAL
LFFNFSHSLTTILSFSSPAALMKTLIKFVDLSRNGRNGSNGNDESSEYETINISLSFS
WAILLIINLTQTYGISVVDVALKYPELSIKNSFIINFISNLPNVSDKYYLEESNVNDS
DMLTKSHNTVQSWLCDLFVNGSITDQLIQNIETRQLANLIPFIVKQVLLSVEIGVLTD
ISSLIGGFEYFLQPLLLVGLIKTFYWLEQFLSCVKNDTISEDILQGIFNLLNTLFNPV
TLNEDSKAFHTAVLRLNAIPLLKVLRKFRVQSQSNYGIYSSDAQGDPNLEPLIAKLVA
VLNVSPVYDVDPRIINSENDYSRKQLGYGKFLILNENPINKIMTNQINSFWSLHSSTY
YNLDYLFELIELVTPKSFLFDVLKTLEYKLATYGVPGSENKRGSLDSEHVFDYFFYFL
VLYDVKTAEEASQLIEYMENDAKKSKGDVDIKGEDLHEKNDSAEVRQETQPKAEATQD
DDFDMLFGENDTSTQAYEEEEENEDNDGNNRTNNVPMIKAEETPSKTNKISILKRHSF
AVLLHERKLLNDLALENGEITKTENEKFISYHDKYLCMLKTCVF"
gene complement(<221104..>225573)
/gene="INO80"
/locus_tag="YGL150C"
/db_xref="GeneID:852728"
mRNA complement(<221104..>225573)
/gene="INO80"
/locus_tag="YGL150C"
/product="chromatin-remodeling ATPase INO80"
/transcript_id="NM_001181015.1"
/db_xref="GeneID:852728"
CDS complement(221104..225573)
/gene="INO80"
/locus_tag="YGL150C"
/experiment="EXISTENCE:direct assay:GO:0006281 DNA repair
[PMID:10952318]"
/experiment="EXISTENCE:direct assay:GO:0006338 chromatin
remodeling [PMID:10952318]"
/experiment="EXISTENCE:direct assay:GO:0006366
transcription by RNA polymerase II [PMID:10952318]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:10952318]"
/experiment="EXISTENCE:direct assay:GO:0019222 regulation
of metabolic process [PMID:29462149]"
/experiment="EXISTENCE:direct assay:GO:0031011 Ino80
complex [PMID:10361278]"
/experiment="EXISTENCE:genetic interaction:GO:0000722
telomere maintenance via recombination [PMID:23390378]"
/experiment="EXISTENCE:mutant phenotype:GO:0000775
chromosome, centromeric region [PMID:23207916]"
/experiment="EXISTENCE:mutant phenotype:GO:0006281 DNA
repair [PMID:10952318]"
/experiment="EXISTENCE:mutant phenotype:GO:0006338
chromatin remodeling [PMID:23207916]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:19620280]"
/experiment="EXISTENCE:mutant phenotype:GO:0006366
transcription by RNA polymerase II [PMID:10952318]"
/experiment="EXISTENCE:mutant phenotype:GO:0031509
subtelomeric heterochromatin formation [PMID:18258182]"
/experiment="EXISTENCE:mutant phenotype:GO:0032006
regulation of TOR signaling [PMID:29462149]"
/experiment="EXISTENCE:mutant phenotype:GO:0033554
cellular response to stress [PMID:19620280]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:17681272]"
/note="Nucleosome spacing factor; subunit of INO80
chromatin remodeling complex; involved in positioning and
spacing of nucleosomes over most genes independently of
transcription; promotes nucleosome shifts in 3' direction;
regulates yeast stress tolerance and cell metabolism
through modulating nitrogen catabolite repression"
/codon_start=1
/product="chromatin-remodeling ATPase INO80"
/protein_id="NP_011365.1"
/db_xref="GeneID:852728"
/db_xref="SGD:S000003118"
/translation="MSLAVLLNKEDKDISDFSKTTAGKSAKKNSRERVADVAPTRVLD
KKQAYLSQLNSEFNRIKRRDSIEQLYQDWKFINLQEFELISEWNQQSKDWQFDNTNDS
QDLHFKKLYRDMSMINKEWAEYQSFKNANLSDIINEKDADEDEEDDEDELEDGEEDME
EDEASTGRHTNGKSMRGNGIQKSRKKDAAAAAAIGKAIKDDQTHADTVVTVNGDENED
GNNGEDEDNDNDNENNNDNDNDNENENDNDSDNDDEEENGEEDEEEEEIEDLDEEDFA
AFEEQDDNDDEDFNPDVEKRRKRSSSSSSSTKLSMNSLSLITSKKINKNITINSDRPK
IVRELIKMCNKNKHQKIKKRRFTNCIVTDYNPIDSKLNIKITLKQYHVKRLKKLINDA
KREREREEALKNNVGLDGNDLDNDEDGSESHKRRKLNNNTANGADDANKRKFNTRHGL
PTYGMKMNAKEARAIQRHYDNTYTTIWKDMARKDSTKMSRLVQQIQSIRSTNFRKTSS
LCAREAKKWQSKNFKQIKDFQTRARRGIREMSNFWKKNEREERDLKKKIEKEAMEQAK
KEEEEKESKRQAKKLNFLLTQTELYSHFIGRKIKTNELEGNNVSSNDSESQKNIDISA
LAPNKNDFHAIDFDNENDEQLRLRAAENASNALAETRAKAKQFDDHANAHEEEEEEDE
LNFQNPTSLGEITIEQPKILACTLKEYQLKGLNWLANLYDQGINGILADEMGLGKTVQ
SISVLAHLAENHNIWGPFLVVTPASTLHNWVNEISKFLPQFKILPYWGNANDRKVLRK
FWDRKNLRYNKNAPFHVMVTSYQMVVTDANYLQKMKWQYMILDEAQAIKSSQSSRWKN
LLSFHCRNRLLLTGTPIQNSMQELWALLHFIMPSLFDSHDEFNEWFSKDIESHAEANT
KLNQQQLRRLHMILKPFMLRRVKKNVQSELGDKIEIDVLCDLTQRQAKLYQVLKSQIS
TNYDAIENAATNDSTSNSASNSGSDQNLINAVMQFRKVCNHPDLFERADVDSPFSFTT
FGKTTSMLTASVANNNSSVISNSNMNLSSMSSNNISNGKFTDLIYSSRNPIKYSLPRL
IYEDLILPNYNNDVDIANKLKNVKFNIFNPSTNYELCLFLSKLTGEPSLNEFFRVSTT
PLLKRVIERTNGPKNTDSLSFKTITQELLEVTRNAPSEGVMASLLNVEKHAYEREYLN
CIQRGYHPNVSAPPVTIEVLGSSHVTNSINNELFDPLISQALSDIPAITQYNMHVKKG
IPVEDFPKTGLFPEPLNKNFSSNISMPSMDRFITESAKLRKLDELLVKLKSEGHRVLI
YFQMTKMMDLMEEYLTYRQYNHIRLDGSSKLEDRRDLVHDWQTNPEIFVFLLSTRAGG
LGINLTAADTVIFYDSDWNPTIDSQAMDRAHRLGQTRQVTVYRLLVRGTIEERMRDRA
KQKEQVQQVVMEGKTQEKNIKTIEVGENDSEVTREGSKSISQDGIKEAASALA"
gene <225572..>225877
/locus_tag="YGL149W"
/db_xref="GeneID:852727"
mRNA <225572..>225877
/locus_tag="YGL149W"
/product="uncharacterized protein"
/transcript_id="NM_001348834.1"
/db_xref="GeneID:852727"
CDS 225572..225877
/locus_tag="YGL149W"
/note="hypothetical protein; conserved among S. cerevisiae
strains; YGL149W is not an essential gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335775.1"
/db_xref="GeneID:852727"
/db_xref="SGD:S000003117"
/translation="MSINALLYVLSLALLIWTGSVVTLLLLLFFCLFLLFFSLHFFCF
TREHVHYTLPPKCHSLKFQFDSIPSSSLSLSPFPFLFFPRLRAVAFASPTLSFFFPI"
gene <226399..>227529
/gene="ARO2"
/locus_tag="YGL148W"
/db_xref="GeneID:852729"
mRNA <226399..>227529
/gene="ARO2"
/locus_tag="YGL148W"
/product="bifunctional chorismate synthase/riboflavin
reductase [NAD(P)H] ARO2"
/transcript_id="NM_001181013.1"
/db_xref="GeneID:852729"
CDS 226399..227529
/gene="ARO2"
/locus_tag="YGL148W"
/EC_number="4.2.3.5"
/EC_number="1.5.1.38"
/experiment="EXISTENCE:direct assay:GO:0004107 chorismate
synthase activity [PMID:8971708]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:19793921|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0042602 riboflavin
reductase (NADPH) activity [PMID:8971708]"
/experiment="EXISTENCE:mutant phenotype:GO:0004107
chorismate synthase activity [PMID:8971708]"
/experiment="EXISTENCE:mutant phenotype:GO:0009073
aromatic amino acid family biosynthetic process
[PMID:8971708]"
/note="Bifunctional chorismate synthase and flavin
reductase; catalyzes the conversion of
5-enolpyruvylshikimate 3-phosphate (EPSP) to form
chorismate, which is a precursor to aromatic amino acids;
protein abundance increases in response to DNA replication
stress"
/codon_start=1
/product="bifunctional chorismate synthase/riboflavin
reductase [NAD(P)H] ARO2"
/protein_id="NP_011367.1"
/db_xref="GeneID:852729"
/db_xref="SGD:S000003116"
/translation="MSTFGKLFRVTTYGESHCKSVGCIVDGVPPGMSLTEADIQPQLT
RRRPGQSKLSTPRDEKDRVEIQSGTEFGKTLGTPIAMMIKNEDQRPHDYSDMDKFPRP
SHADFTYSEKYGIKASSGGGRASARETIGRVASGAIAEKFLAQNSNVEIVAFVTQIGE
IKMNRDSFDPEFQHLLNTITREKVDSMGPIRCPDASVAGLMVKEIEKYRGNKDSIGGV
VTCVVRNLPTGLGEPCFDKLEAMLAHAMLSIPASKGFEIGSGFQGVSVPGSKHNDPFY
FEKETNRLRTKTNNSGGVQGGISNGENIYFSVPFKSVATISQEQKTATYDGEEGILAA
KGRHDPAVTPRAIPIVEAMTALVLADALLIQKARDFSRSVVH"
gene complement(<227754..>228329)
/gene="RPL9A"
/locus_tag="YGL147C"
/db_xref="GeneID:852730"
mRNA complement(<227754..>228329)
/gene="RPL9A"
/locus_tag="YGL147C"
/product="60S ribosomal protein uL6 RPL9A"
/transcript_id="NM_001181012.3"
/db_xref="GeneID:852730"
CDS complement(227754..228329)
/gene="RPL9A"
/locus_tag="YGL147C"
/experiment="EXISTENCE:direct assay:GO:0002181 cytoplasmic
translation [PMID:18782943]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:18782943]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:18782943]"
/note="Ribosomal 60S subunit protein L9A; homologous to
mammalian ribosomal protein L9 and bacterial L6; RPL9A has
a paralog, RPL9B, that arose from a single-locus
duplication"
/codon_start=1
/product="60S ribosomal protein uL6 RPL9A"
/protein_id="NP_011368.3"
/db_xref="GeneID:852730"
/db_xref="SGD:S000003115"
/translation="MKYIQTEQQIEVPEGVTVSIKSRIVKVVGPRGTLTKNLKHIDVT
FTKVNNQLIKVAVHNGGRKHVAALRTVKSLVDNMITGVTKGYKYKMRYVYAHFPINVN
IVEKDGAKFIEVRNFLGDKKIRNVPVRDGVTIEFSTNVKDEIVLSGNSVEDVSQNAAD
LQQICRVRNKDIRKFLDGIYVSHKGFITEDL"
gene complement(<228751..>229686)
/gene="RRT6"
/locus_tag="YGL146C"
/db_xref="GeneID:852731"
mRNA complement(<228751..>229686)
/gene="RRT6"
/locus_tag="YGL146C"
/product="Rrt6p"
/transcript_id="NM_001181011.1"
/db_xref="GeneID:852731"
CDS complement(228751..229686)
/gene="RRT6"
/locus_tag="YGL146C"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:40704575]"
/note="Member of the p24 family; forms complex with Erp3p,
Erp5p, and Emp24p; non-essential gene identified in a
screen for mutants with increased levels of rDNA
transcription"
/codon_start=1
/product="Rrt6p"
/protein_id="NP_011369.1"
/db_xref="GeneID:852731"
/db_xref="SGD:S000003114"
/translation="MEKASLNIAQTKEIPLTDRRMGLRGWKACSRPHLLGRILLFMSI
LFITSAELSSDVSSREVYMPIFNNKLSFKVPPIKRSLLLGAALYEDFEYSSNNSASDG
AFCTVFNAGMNDASREVVFEIHVMDVLQEETDSSRFGGTSHERGRQSLGFSVFNNKNG
DLLRSKKNLASGTSVIEVNPGNCNEFLICFINLVYDGSWSSIDTEKSVTIKMTYNDKL
DPDMLLHLVNQMTPQVVKALNTVSDGLFQIVSDTTLLQMESDRRDINEATYSYLIVGF
VSLMVAQLISNIIVTTYLIIKIKSNPSSHIKKKGL"
gene <230243..>232348
/gene="TIP20"
/locus_tag="YGL145W"
/gene_synonym="TIP1"
/db_xref="GeneID:852732"
mRNA <230243..>232348
/gene="TIP20"
/locus_tag="YGL145W"
/gene_synonym="TIP1"
/product="Tip20p"
/transcript_id="NM_001181010.2"
/db_xref="GeneID:852732"
CDS 230243..232348
/gene="TIP20"
/locus_tag="YGL145W"
/gene_synonym="TIP1"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:11739780]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0070939 Dsl1/NZR
complex [PMID:19151722]"
/experiment="EXISTENCE:mutant phenotype:GO:0006890
retrograde vesicle-mediated transport, Golgi to
endoplasmic reticulum [PMID:9639310]"
/experiment="EXISTENCE:mutant phenotype:GO:0006914
autophagy [PMID:30837368]"
/experiment="EXISTENCE:physical interaction:GO:0070939
Dsl1/NZR complex [PMID:15958492]"
/note="Peripheral membrane protein required for COPI
vesicle fusion to the ER; mediates Sey1p-independent
homotypic ER fusion; prohibits back-fusion of COPII
vesicles with the ER; forms a tethering complex with
Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and
Use1p"
/codon_start=1
/product="Tip20p"
/protein_id="NP_116577.1"
/db_xref="GeneID:852732"
/db_xref="SGD:S000003113"
/translation="MNGIDDLLNINDRIKQVQNERNELASKLQNLKQSLASNDTEVAL
SEVIAQDIIEVGASVEGLEQLRAKYGDLQILNKLEKVAVQQTQMQAGVDKLDSFERQL
DELAEQPPDQFTLDDVKALHSKLTSVFATVPQINNIDSQYAAYNKLKSKVTGKYNDVI
IQRLATNWSNTFDQKLLEAQWDTQKFASTSVGLVKCLRENSTKLYQLSLLYLPLEEET
QNGDSERPLSRSNNNQEPVLWNFKSLANNFNVRFTYHFHATSSSSKIETYFQFLNDYL
AENLYKCINIFHDDCNGLTKPVIHEQFINYVLQPIRDKVRSTLFQNDLKTLIVLISQI
LATDKNLLNSFHYHGLGLVSLISDEVWEKWINYEVEMANRQFINITKNPEDFPKSSQN
FVKLINKIYDYLEPFYDLDFDLLVRYKLMTCSLIFMNLTSSYLDYILTVDSLNETRTK
EQELYQTMAKLQHVNFVYRKIKSLSSNFIFIQLTDIVNSTESKKYNSLFQNVENDYEK
AMSTDMQNSIVHRIQKLLKETLRNYFKISTWSTLEMSVDENIGPSSVPSAELVNSINV
LRRLINKLDSMDIPLAISLKVKNELLNVIVNYFTESILKLNKFNQNGLNQFLHDFKSL
SSILSLPSHATNYKCMSLHELVKILKLKYDPNNQQFLNPEYIKTGNFTSLKEAYSIKY
LKDTKIQDALYRIIYGNIL"
rep_origin 231907..231981
/gene="TIP20"
/locus_tag="YGL145W"
/gene_synonym="TIP1"
/note="ARS701; ARS containing only an imperfect match to
the core consensus sequence"
/db_xref="SGD:S000007642"
gene complement(<232450..>234507)
/gene="ROG1"
/locus_tag="YGL144C"
/db_xref="GeneID:852733"
mRNA complement(<232450..>234507)
/gene="ROG1"
/locus_tag="YGL144C"
/product="putative lipase ROG1"
/transcript_id="NM_001181009.1"
/db_xref="GeneID:852733"
CDS complement(232450..234507)
/gene="ROG1"
/locus_tag="YGL144C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:25433290]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:25433290]"
/experiment="EXISTENCE:direct assay:GO:0047372
monoacylglycerol lipase activity [PMID:25433290]"
/experiment="EXISTENCE:mutant phenotype:GO:0006629 lipid
metabolic process [PMID:25433290]"
/note="Lipase with specificity for monoacylglycerol;
preferred substrate is 1-oleoylglycerol; null mutation
affects lipid droplet morphology and overexpression causes
increased accumulation of reactive oxygen species"
/codon_start=1
/product="putative lipase ROG1"
/protein_id="NP_011371.1"
/db_xref="GeneID:852733"
/db_xref="SGD:S000003112"
/translation="MSLTPTNEILFHYKSSVKVGELERYVITYHLYDGEEIPPDLNLN
SLWLKVRNMNPLSYRAAYLMGPFMLYCDVKTAQYHHSQKIVASVDYPKFEPNVQTQQD
FVAELSVHNIRQKYVWIADVMSQILFTTNTNVTYEVTIGTSKESVENPHDLPSHLGSY
SPKLTVNRLTTLDLWNLPVQITTPQKKKHLVVLTHGLHSNVSTDLVYIMEQIYKAQKN
YPHEQIVVKGYRGNVCQTEKGVKYLGTRLAEYIIQDLYDESIRKISFVGHSLGGLIQA
FAIAYIYEVYPWFFKKVNPINFITLASPLLGIVTDNPAYIKVLLSFGVIGKTGQDLGL
ENDVEVGKPLLYLLSGLPLIEILRRFKRRTVYANAINDGIVPLYTASLLFLDYNDILE
QLQKLKENSKKSPLINDASTPVNQDFFNKTFISPLTKMLSILAPQKFPTENGSEIPKV
SFFESASSILLPPLPERAYIMDPDSRDPVIIHDKIYNEDDIPQSEFDIEDGFFGKKNI
LLQAFFAGKKERAKYRNLEETIARRWHEGMAWRKVVVALKPDAHNNIIVRRKFANAYG
WPVIDHLIDVHFNGDDDDDNDENDDINSTQVVEPIQSVTEGKKKYRKAENIPQEYGWL
NKVETNGVFDEGPTGMISTVGEIVEALAKRGFSAVIDRRNASEDPNDEVLRFEEMNSD
LVQ"
gene complement(<234717..>235958)
/gene="MRF1"
/locus_tag="YGL143C"
/db_xref="GeneID:852734"
mRNA complement(<234717..>235958)
/gene="MRF1"
/locus_tag="YGL143C"
/product="Mrf1p"
/transcript_id="NM_001181008.3"
/db_xref="GeneID:852734"
CDS complement(234717..235958)
/gene="MRF1"
/locus_tag="YGL143C"
/experiment="EXISTENCE:direct assay:GO:0003747 translation
release factor activity [PMID:10748224]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:11502169|PMID:8265342|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:genetic interaction:GO:0003747
translation release factor activity [PMID:1475194]"
/experiment="EXISTENCE:genetic interaction:GO:0070126
mitochondrial translational termination [PMID:1475194]"
/experiment="EXISTENCE:mutant phenotype:GO:0005739
mitochondrion [PMID:1475194]"
/experiment="EXISTENCE:mutant phenotype:GO:0006415
translational termination [PMID:1475194]"
/experiment="EXISTENCE:mutant phenotype:GO:0032543
mitochondrial translation [PMID:14734569]"
/experiment="EXISTENCE:mutant phenotype:GO:0070126
mitochondrial translational termination [PMID:15827612]"
/note="Mitochondrial translation release factor RF1;
involved in stop codon recognition and hydrolysis of
peptidyl-tRNA bond during mitochondrial translation; lack
of MRF1 causes mitochondrial genome instability"
/codon_start=1
/product="Mrf1p"
/protein_id="NP_011372.3"
/db_xref="GeneID:852734"
/db_xref="SGD:S000003111"
/translation="MWLSKFQFPSRSIFKGVFLGHKLPLLVRLTSTTTNSKSNGSIPT
QYTELSPLLVKQAEKYEAELKDLDKDLSCGIHFDVNKQKHYAKLSALTDTFIEYKEKL
NELKSLQEMIVSDPSLRAEAEQEYAELVPQYETTSSRLVNKLLPPHPFADKPSLLELR
PGVGGIEAMIFTQNLLDMYIGYANYRKWKYRIISKNENESGSGIIDAILSIEEAGSYD
RLRFEAGVHRVQRIPSTETKGRTHTSTAAVVVLPQIGDESAKSIDAYERTFKPGEIRV
DIMRASGKGGQHVNTTDSAVRLTHIPSGIVVSMQDERSQHKNKAKAFTILRARLAEKE
RLEKEEKERKARKSQVSSTNRSDKIRTYNFPQNRITDHRCGFTLLDLPGVLSGERLDE
VIEAMSKYDSTERAKELLESN"
gene complement(<236269..>238119)
/gene="GPI10"
/locus_tag="YGL142C"
/db_xref="GeneID:852735"
mRNA complement(<236269..>238119)
/gene="GPI10"
/locus_tag="YGL142C"
/product="putative glycosylphosphatidylinositol-alpha 1,2
mannosyltransferase"
/transcript_id="NM_001181007.1"
/db_xref="GeneID:852735"
CDS complement(236269..238119)
/gene="GPI10"
/locus_tag="YGL142C"
/experiment="EXISTENCE:mutant phenotype:GO:0000026
alpha-1,2-mannosyltransferase activity
[PMID:9639537|PMID:9576863]"
/experiment="EXISTENCE:mutant phenotype:GO:0006506 GPI
anchor biosynthetic process [PMID:9759715]"
/note="Integral membrane protein involved in GPI anchor
synthesis; putative alpha 1,2 mannosyltransferase required
for addition of the third mannose onto the
glycosylphosphatidylinositol (GPI) core structure; human
PIG-Bp is a functional homolog"
/codon_start=1
/product="putative glycosylphosphatidylinositol-alpha 1,2
mannosyltransferase"
/protein_id="NP_011373.1"
/db_xref="GeneID:852735"
/db_xref="SGD:S000003110"
/translation="MAHEVHRIKPKLGRTQIFWVFLAFRVLNAVLTRTFFQADEFWQA
LEPAHWKAFKYGELTWEWKFGVRSYLFPMIFELTYRLVSLSSILLHYALLLLSTIGSD
LLILLLPKYELSWQVAEDLKRLPFDVTRSFEYYGVIYAPKIVMAVLASIGEYYIVRFV
QKLYLLTLDKRNEKEEEERRSGLSEITKFALLLSLTNFFNCFFITRTFINSFEMILTS
IALYYWDWTGGQMIKESSFTKSLIFAFLACLQRPSSGLIWVIPSISLILNLVGKKQYH
LLFITFSKVLRSFFLVFTANAIIDMYFYEKVTFPFFRFLKFNFTTPLSKFYGVAPWHF
HFFQSLPIVLGASIPAFAFGLFFPLSKRSFPKKYLNPFFQVKLTILLNLLVYSTLPHK
EFRFIFPLQPLFILISSFGLLRLDRDYWKRLSGLKSLLWLVPFVSVFIALLLDTFHES
GSIEVMKFLHEEPEIDSLGFIMPCHSTPGQSYLHRSDIQDLWSITCNPPLHLLGDPEA
YSKLETYMDESDHLYDDISAFIYKNFPPPFRKDLRSPGKTYSHEWPTYLVVFEHMENA
FLKDFLKDSSYIEYNRFFNSLAHWDSRRSGDIIIYYKLPFDYSDIPAADI"
gene <238353..>241085
/gene="HUL5"
/locus_tag="YGL141W"
/db_xref="GeneID:852736"
mRNA <238353..>241085
/gene="HUL5"
/locus_tag="YGL141W"
/product="ubiquitin-ubiquitin ligase HUL5"
/transcript_id="NM_001181006.1"
/db_xref="GeneID:852736"
CDS 238353..241085
/gene="HUL5"
/locus_tag="YGL141W"
/EC_number="2.3.2.26"
/experiment="EXISTENCE:direct assay:GO:0000209 protein
polyubiquitination [PMID:17190603]"
/experiment="EXISTENCE:direct assay:GO:0000502 proteasome
complex [PMID:12408819]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0034450
ubiquitin-ubiquitin ligase activity [PMID:17190603]"
/experiment="EXISTENCE:mutant phenotype:GO:0005829 cytosol
[PMID:25344756]"
/experiment="EXISTENCE:mutant phenotype:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:25344756]"
/experiment="EXISTENCE:mutant phenotype:GO:0010994 free
ubiquitin chain polymerization [PMID:22497224]"
/experiment="EXISTENCE:mutant phenotype:GO:0033554
cellular response to stress [PMID:22497224]"
/experiment="EXISTENCE:mutant phenotype:GO:0034605
cellular response to heat [PMID:25344756]"
/experiment="EXISTENCE:mutant phenotype:GO:0036503 ERAD
pathway [PMID:17190603]"
/note="Multiubiquitin chain assembly factor (E4);
proteasome processivity factor that elongates polyUb
chains on substrates, opposing Ubp6p, a branched
polyubiquitin protease; required for retrograde transport
of misfolded proteins during ERAD; required for
ubiquitination of a subset of cytosolic misfolded proteins
upon heat shock"
/codon_start=1
/product="ubiquitin-ubiquitin ligase HUL5"
/protein_id="NP_011374.1"
/db_xref="GeneID:852736"
/db_xref="SGD:S000003109"
/translation="MLNFTGQTRRRNVNLGNRTRNSKKDLLEKAKRERERRAQDKLKE
DASKTIQKSIRRHFSNVRLFKNTFTSSQLVHMIPAYGGKLIYYISQYDLQQLLKLSHN
FLSSYPNSLGNRQLLSLLKLYQDDALVAETLSDLNMDCPTVDEFLDSLSVYLCRASFL
SYSSASKLADVIEAWEVMHSSASISIFSISIGSYEKRPFALQFYCILAERNLLPQLIN
TNPILWDNMAKTYSHCSKGGQKNIAKLLIPNFNNHIAPSVLRSDNDYVLKFYEKAFID
EVIATTANYVSDEDHVKNLMCYIASSPNQSCKNSVLITLLSNKDFVRRLSWEFFHTKF
NASKTEAHPLFSVLAQLIDMHLLISTDRELLDYNSVIPIEELKKFTSTLKDFTFRQYW
ELPKSERNPMLKEAVPLLSKVYERDSRLHFLSTENNPTYWENSEKQFLNLRFYEELQE
YEDLYREHLEEESDEDMEKEIDLDKERPPLKSLLLNKMKKRLKSSLRFRKLEILLELP
FFIPFEERVDLFYMFIALDKKRLSLDDDHNLINMFTPWASTGMRKQSAIISRDNVLED
AFNAFNSIGERFKASLDVTFINEFGEEAGIDGGGITKEFLTTVSDEGFKDPKHELFRT
NDRYELYPSVVYDATKLKYIWFLGKVVGKCLYEHVLIDVSFADFFLKKLLNYSNGFLS
SFSDLGSYDSVLYNNLIKLLNMTTDEIKSLDLTFEIDEPESSAKVVDLIPNGSKTYVT
KDNVLLYVTKVTDYKLNKRCFKPVSAFHGGLSVIIAPHWMEMFNSIELQMLISGERDN
IDLDDLKSNTEYGGYKEEDQTIVDFWEVLNEFKFEEKLNFLKFVTSVPQAPLQGFKAL
DPKFGIRNAGTEKYRLPTASTCVNLLKLPDYRNKTILREKLLYAINSGARFDLS"
rep_origin 241085..241353
/note="ARS712; Replication origin; identified in multiple
array studies, confirmed by plasmid-based recombinational
ARS assay"
/db_xref="SGD:S000130164"
gene complement(<241353..>245012)
/locus_tag="YGL140C"
/db_xref="GeneID:852737"
mRNA complement(<241353..>245012)
/locus_tag="YGL140C"
/product="uncharacterized protein"
/transcript_id="NM_001181005.1"
/db_xref="GeneID:852737"
CDS complement(241353..245012)
/locus_tag="YGL140C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/note="hypothetical protein; non-essential gene; contains
multiple predicted transmembrane domains"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011375.1"
/db_xref="GeneID:852737"
/db_xref="SGD:S000003108"
/translation="MSLKSKLTKIQKLWLYYFPCDRILAKRICKSTVNTTVAFIFCLI
PKITAHLGAAPAMLPMISVIVHPGRRVGGTIHGAIYCITGLIFGLAYAIFGRFLAQRC
LGSSWHELTEAQQHVLHYKRYEAGLAILAVFEVIMLFFHGWMRSVSHYYFGIVFPLFV
VVHFAFMDPLNETAGTIAKAYSTPFYLGIAMSIFWNLVLFPEWGTTYLGNTTIDAMNE
LHKSIDYSINFFIAVDPHNSSQLYSRDPVSLGKLLKMKSLISSKVNNCRVVLHECIYE
FTYAYVSPTKLKPIISTLENLTVFINGLVNTCQLEFILLARHDNKLRPDDVAALTLPK
NKEISFANAEKLLKVIDKLHPVIYSLHRTMSECMYIAKLVLAHAFDVKVSRVHSCSMF
KDGNFPTFSNNANNLPNDVDIQNKINDLKQALEECKAKFKSEMLSFDIDIMSPSDEMF
LLSSFLLNFRQTADSTLVIMESVKDILVKRQIQEKKGWLRGKRLWFLVLTNYETFSIW
LKGDRNSVTENDTLKGTFNGNTNGFAHDTVIRRPDYEENELLSQKVSSNKNIVKDDAS
LDLPMTSEPKGNSSSTSDTSSSPLTLTKTTTFGTNRTSRRQGRFSFMSMLISIDKFCE
VSHPHFRFGFQVAIALMLASFPMFIPKTRQWYIDYRGTWIGFVCILCLEPSVGGTFWV
FFLRAVGVIFGAAWGYLSYVAAVNQTNPYLETVITVFGAIPGFYYLLGTPYVKAAIIE
IISIYIVMLAAILPSQDDILTSFAKRCLAVGYGGGVALIVQVFFFPLKAREQLNEEIS
FVCGCISEMELLYATGLEGEQVASSMSEEKYKKIEKISKSAKEALARATAYKGLTRQE
PRLKGEYTELENVFTQVIFIQKQIIERIDTISLLRKQNGSAVIEEFNSVVYPYRRQMV
GSISCLMRALQEAFINKTPLPQFLPSARIAHRRLINKVRQTLRIRYPGQISNLSDKAR
KPNEGDYADGKDEDDDDNEGLVMKMNRRGQANTTANPHEYVLKEKFLSWNASSAASEE
IIEYIEELLNLTKILVGVNEFKYGFLSRPLYEDWAAEAVTGFDNFINGKSNPMNTRRN
RTPFDGTSIISEGNESLQSTNSNESQISPDSTRSYEPECPVAYEKNDNPAALNLLRIA
SHKAGQNADGLPKTFRNRAFSITSTSGQLSSLSRHSTLGNADPNYLNDDDESSDDDLP
LALKMVLSHMKEKKD"
gene <245716..>248124
/gene="FLC3"
/locus_tag="YGL139W"
/db_xref="GeneID:852738"
mRNA <245716..>248124
/gene="FLC3"
/locus_tag="YGL139W"
/product="putative flavin adenine dinucleotide
transporter"
/transcript_id="NM_001181004.3"
/db_xref="GeneID:852738"
CDS 245716..248124
/gene="FLC3"
/locus_tag="YGL139W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:16717099]"
/experiment="EXISTENCE:genetic interaction:GO:0030148
sphingolipid biosynthetic process [PMID:27462707]"
/note="Putative FAD transporter, similar to Flc1p and
Flc2p; localized to the ER; FLC3 has a paralog, FLC1, that
arose from the whole genome duplication"
/codon_start=1
/product="putative flavin adenine dinucleotide
transporter"
/protein_id="NP_011376.3"
/db_xref="GeneID:852738"
/db_xref="SGD:S000003107"
/translation="MRFLQVYKSSALIGLIILLASKVNLAEAKRKLVATSLVTCMENS
QLSANSFDVVFNPDDRSLHYDLDMSTQIDSYIFADIDVYAYGFKIITKNVDLCSINWK
QFCPVHPGNIQIDSIEYISSEYVNEIPGIAYQVPDIDAYARVKITNNVSEYLACIQIY
FSNGKTVSQIGVKWATAVVAGIGLLLSAILSTFGNSTAASHISANTMSLFLYFQSVVV
VAMQHVHRVPPIAAAWAENLVWSMGLIRISFMQRIFRWYVQSTGGTPSLYLTSTSMSV
LAQRSWQYLMELPLIKRATNVLYGNANTLIFRGIKRLGYKMGIENTSIVCTGFTFFVL
CGYVLAGFIIVFKCCVELATRLGWIQKARFWEFRKQWRMILKGALLRYIYIGFVQLTI
LSFWEFTERDSPAVIVIACLFILLSCGLMLWAAWRTVFFARRSVALYNNPAALLYGDE
YVLHKYGFFYTMFNANHYWWNIVLLSYIFVKSLLVGFAQASGQTQVLFMFILDLFYFV
AIIYYKPYLDRPTNIMNILIATVTVVNSFLFMFFSDLFNQSYKVAAIMGWIFFIMNAA
FSFILLMMILAFAGMMLFSKNPDLRFKPAKDDRTSFQRNTMKPEGTVNRSVANELLAL
GNVAKDHDDNSDYESNDTGVNDELKQAQDETTPTTVTSSDDNKPTFSEKILSKFSRPK
NENASTDALRVEAPKQQTFPHNLTNLSRENLSTLGSKPYPGHTRSQSDAHNGLINSFE
EEDTSSNTDPFHDSTEGDLLDTSSSDGGFRSQNYVRDDSINSLGNNKQPLRKPPGFFD
EGFM"
gene complement(<248494..>249531)
/locus_tag="YGL138C"
/db_xref="GeneID:852739"
mRNA complement(<248494..>249531)
/locus_tag="YGL138C"
/product="uncharacterized protein"
/transcript_id="NM_001181003.1"
/db_xref="GeneID:852739"
CDS complement(248494..249531)
/locus_tag="YGL138C"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:24390141]"
/note="hypothetical protein; has no significant sequence
similarity to any known protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011377.1"
/db_xref="GeneID:852739"
/db_xref="SGD:S000003106"
/translation="MYNFLEFFFFFITYTLFKSTFVQGKSSFPGHDVCKFEDQNFQTE
FFLNVLKGDKLQNLKQEYEQYKKQSTLYTGFVIEKQYEYQVAPLQINNFLQVTFCKGG
KPIWNHILPFQKDLDWAEPLCIPAQEDDTISQNSSVCFKFARVQKYTQRNVTLYFPNK
FVGFVFVCNSSKTHPPTQNNFETIPLTPIISDDIRDYKISWSIKGRITKVVPYLHSLS
IPMSKYEMLIHSDKNISNELEYKSLTSEFWKSYKNLRKFKNKEMSWINEINPMEQYDS
SGNENVPNFHEKLDRTINRIAQNIERPHDALIRAIAAHNRNSNSGNITRRKYLRRKIS
KMLKNKIPLKN"
gene <249869..>252738
/gene="SEC27"
/locus_tag="YGL137W"
/db_xref="GeneID:852740"
mRNA join(<249869..249886,250087..>252738)
/gene="SEC27"
/locus_tag="YGL137W"
/product="coatomer subunit beta'"
/transcript_id="NM_001181002.1"
/db_xref="GeneID:852740"
CDS join(249869..249886,250087..252738)
/gene="SEC27"
/locus_tag="YGL137W"
/experiment="EXISTENCE:direct assay:GO:0030126 COPI
vesicle coat [PMID:21435344]"
/experiment="EXISTENCE:direct assay:GO:0043130 ubiquitin
binding [PMID:21070969]"
/experiment="EXISTENCE:mutant phenotype:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:7929113]"
/experiment="EXISTENCE:mutant phenotype:GO:0006890
retrograde vesicle-mediated transport, Golgi to
endoplasmic reticulum [PMID:8001155]"
/experiment="EXISTENCE:mutant phenotype:GO:0008298
intracellular mRNA localization [PMID:27068463]"
/experiment="EXISTENCE:mutant phenotype:GO:0032511 late
endosome to vacuole transport via multivesicular body
sorting pathway [PMID:17101773]"
/experiment="EXISTENCE:physical interaction:GO:0032511
late endosome to vacuole transport via multivesicular body
sorting pathway [PMID:17101773]"
/note="Essential beta'-coat protein of the COPI coatomer;
involved in ER-to-Golgi and Golgi-to-ER transport;
contains WD40 domains that mediate cargo selective
interactions; 45% sequence identity to mammalian
beta'-COP"
/codon_start=1
/product="coatomer subunit beta'"
/protein_id="NP_011378.1"
/db_xref="GeneID:852740"
/db_xref="SGD:S000003105"
/translation="MKLDIKKTFSNRSDRVKGIDFHPTEPWVLTTLYSGRVELWNYET
QVEVRSIQVTETPVRAGKFIARKNWIIVGSDDFRIRVFNYNTGEKVVDFEAHPDYIRS
IAVHPTKPYVLSGSDDLTVKLWNWENNWALEQTFEGHEHFVMCVAFNPKDPSTFASGC
LDRTVKVWSLGQSTPNFTLTTGQERGVNYVDYYPLPDKPYMITASDDLTIKIWDYQTK
SCVATLEGHMSNVSFAVFHPTLPIIISGSEDGTLKIWNSSTYKVEKTLNVGLERSWCI
ATHPTGRKNYIASGFDNGFTVLSLGNDEPTLSLDPVGKLVWSGGKNAAASDIFTAVIR
GNEEVEQDEPLSLQTKELGSVDVFPQSLAHSPNGRFVTVVGDGEYVIYTALAWRNKAF
GKCQDFVWGPDSNSYALIDETGQIKYYKNFKEVTSWSVPMHSAIDRLFSGALLGVKSD
GFVYFFDWDNGTLVRRIDVNAKDVIWSDNGELVMIVNTNSNGDEASGYTLLFNKDAYL
EAANNGNIDDSEGVDEAFDVLYELSESITSGKWVGDVFIFTTATNRLNYFVGGKTYNL
AHYTKEMYLLGYLARDNKVYLADREVHVYGYEISLEVLEFQTLTLRGEIEEAIENVLP
NVEGKDSLTKIARFLEGQEYYEEALNISPDQDQKFELALKVGQLTLARDLLTDESAEM
KWRALGDASLQRFNFKLAVEAFTNAHDLESLFLLHSSFNNKEGLVTLAKDAERAGKFN
LAFNAYWIAGDIQGAKDLLIKSQRFSEAAFLGSTYGLGDDAVNDIVTKWKENLILNGK
NTVSERVCGAEGLPGSSSSGDAQPLIDLDSTPAPEQADENKEAEVEDSEFKESNSEAV
EAEKKEEEAPQQQQSEQQPEQGEAVPEPVEEES"
gene complement(<252897..>253859)
/gene="MRM2"
/locus_tag="YGL136C"
/db_xref="GeneID:852741"
mRNA complement(<252897..>253859)
/gene="MRM2"
/locus_tag="YGL136C"
/product="21S rRNA (uridine2791-2'-O) methyltransferase"
/transcript_id="NM_001181001.1"
/db_xref="GeneID:852741"
CDS complement(252897..253859)
/gene="MRM2"
/locus_tag="YGL136C"
/EC_number="2.1.1.168"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:11867542]"
/experiment="EXISTENCE:direct assay:GO:0008650 rRNA
(uridine-2'-O-)-methyltransferase activity
[PMID:11867542]"
/experiment="EXISTENCE:direct assay:GO:0031167 rRNA
methylation [PMID:11867542]"
/experiment="EXISTENCE:mutant phenotype:GO:0008650 rRNA
(uridine-2'-O-)-methyltransferase activity
[PMID:11867542|PMID:14636587]"
/experiment="EXISTENCE:mutant phenotype:GO:0031167 rRNA
methylation [PMID:11867542|PMID:14636587]"
/note="Mitochondrial 2' O-ribose methyltransferase;
required for methylation of U(2791) in 21S rRNA; MRM2
deletion confers thermosensitive respiration and loss of
mitochondrial DNA; has similarity to Spb1p and Trm7p, and
to E. coli FtsJ/RrmJ"
/codon_start=1
/product="21S rRNA (uridine2791-2'-O) methyltransferase"
/protein_id="NP_011379.1"
/db_xref="GeneID:852741"
/db_xref="SGD:S000003104"
/translation="MILVYNRIRSIISSSLGRIHVRYNSNSQNRWLNRQLKDPYTKEA
KVQNLRSRAAFKLMQIDDKYRLFSKNRTDQRILDLGYAPGAWSQVARQRSSPNSMILG
VDILPCEPPHGVNSIQANILAKRTHDLIRLFFSKHFQLNRHDDLHKDHGYFQNMLEEE
LTHVKDTELYREIFTSDDIYETPNTNSTLIEREKFPVDVIISDMYEPWPQTTGFWNNI
TNQAYFRMANTSGVSIRDHYQSIDLCDAALVTAIDLLRPLGSFVCKLYTGEEENLFKK
RMQAVFTNVHKFKPDASRDESKETYYIGLKKKRNVDKLDVFSNS"
gene <254641..>255294
/gene="RPL1B"
/locus_tag="YGL135W"
/gene_synonym="SSM2"
/db_xref="GeneID:852742"
mRNA <254641..>255294
/gene="RPL1B"
/locus_tag="YGL135W"
/gene_synonym="SSM2"
/product="60S ribosomal protein uL1 RPL1B"
/transcript_id="NM_001181000.3"
/db_xref="GeneID:852742"
CDS 254641..255294
/gene="RPL1B"
/locus_tag="YGL135W"
/gene_synonym="SSM2"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:11983894]"
/experiment="EXISTENCE:curator inference:GO:0003735
structural constituent of ribosome [PMID:11983894]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:11983894]"
/experiment="EXISTENCE:mutant phenotype:GO:0000055
ribosomal large subunit export from nucleus
[PMID:31439809]"
/note="Ribosomal 60S subunit protein L1B; N-terminally
acetylated; homologous to mammalian ribosomal protein L10A
and bacterial L1; RPL1B has a paralog, RPL1A, that arose
from the whole genome duplication; rpl1a rpl1b double null
mutation is lethal; ribosomes containing Rpl1b are more
efficient in translation of respiration-related proteins"
/codon_start=1
/product="60S ribosomal protein uL1 RPL1B"
/protein_id="NP_011380.3"
/db_xref="GeneID:852742"
/db_xref="SGD:S000003103"
/translation="MSKITSSQVREHVKELLKYSNETKKRNFLETVELQVGLKNYDPQ
RDKRFSGSLKLPNCPRPNMSICIFGDAFDVDRAKSCGVDAMSVDDLKKLNKNKKLIKK
LSKKYNAFIASEVLIKQVPRLLGPQLSKAGKFPTPVSHNDDLYGKVTDVRSTIKFQLK
KVLCLAVAVGNVEMEEDVLVNQILMSVNFFVSLLKKNWQNVGSLVVKSSMGPAFRLY"
gene <255663..>256964
/gene="PCL10"
/locus_tag="YGL134W"
/db_xref="GeneID:852743"
mRNA <255663..>256964
/gene="PCL10"
/locus_tag="YGL134W"
/product="Pcl10p"
/transcript_id="NM_001180999.1"
/db_xref="GeneID:852743"
CDS 255663..256964
/gene="PCL10"
/locus_tag="YGL134W"
/experiment="EXISTENCE:direct assay:GO:0016538
cyclin-dependent protein serine/threonine kinase regulator
activity [PMID:9584169]"
/experiment="EXISTENCE:genetic interaction:GO:0045719
negative regulation of glycogen biosynthetic process
[PMID:9584169]"
/experiment="EXISTENCE:mutant phenotype:GO:0045719
negative regulation of glycogen biosynthetic process
[PMID:9584169]"
/experiment="EXISTENCE:physical interaction:GO:0000307
cyclin-dependent protein kinase holoenzyme complex
[PMID:9584169]"
/note="Pho85p cyclin; recruits, activates, and targets
Pho85p cyclin-dependent protein kinase to its substrate;
PCL10 has a paralog, PCL8, that arose from the whole
genome duplication"
/codon_start=1
/product="Pcl10p"
/protein_id="NP_011381.1"
/db_xref="GeneID:852743"
/db_xref="SGD:S000003102"
/translation="MDMTKNHTTDTEEFDDGDIRPVSLGIVDDYNASFELPLKPKFLQ
SENFSDLTSEWDQSRSNTPGLAEGKTEKAQPCGTTDSSKNRIHVEQLLESANEMNNYL
AQNIENINNFQVGLLNGGKGLYSSMGDDSSACINGTNFSSTSNFELSDDELEDTTGCT
SSIFDKDLFHQQNGLSIPRRRSPLFKSPTASFEIGDATDVEEQDIDDSIFSECSSITS
FDMGGLHISLPHDEEEDQEKTKSESENPLLHGIPVDVEVPHISVDEALANFKETIELL
LKLSGNRKCTGFNTRVEKKEYSNFYMKSKPTLSSADFLKRIQDKCEYQPTVYLVATFL
IDTLFLTRDGNNILQLKLNLQEKEVHRMIIAAVRLSTKLLEDFVHSHEYFSKVCGISK
RLLTKLEVSLLICVCNTKLMVSNRKLAASKLLLNELRSFCV"
gene <257707..>261501
/gene="ITC1"
/locus_tag="YGL133W"
/db_xref="GeneID:852744"
mRNA <257707..>261501
/gene="ITC1"
/locus_tag="YGL133W"
/product="Itc1p"
/transcript_id="NM_001180998.1"
/db_xref="GeneID:852744"
CDS 257707..261501
/gene="ITC1"
/locus_tag="YGL133W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:12624196]"
/experiment="EXISTENCE:mutant phenotype:GO:0031509
subtelomeric heterochromatin formation [PMID:14673157]"
/experiment="EXISTENCE:mutant phenotype:GO:0071444
cellular response to pheromone [PMID:12624196]"
/experiment="EXISTENCE:physical interaction:GO:0008623
CHRAC [PMID:14673157]"
/note="Subunit of ATP-dependent Isw2p-Itc1p chromatin
remodeling complex; required for repression of a-specific
genes, repression of early meiotic genes during mitotic
growth, and repression of INO1; similar to mammalian
Acf1p, the regulatory subunit of the mammalian
ATP-utilizing chromatin assembly and modifying factor
(ACF) complex; ITC1 has a paralog, YPL216W, that arose
from the whole genome duplication"
/codon_start=1
/product="Itc1p"
/protein_id="NP_011382.1"
/db_xref="GeneID:852744"
/db_xref="SGD:S000003101"
/translation="MVLYKRKPILLPDPKPLPLDLNVQVWHIEETGEWFSSYEEFLER
FDFYTRHHFTCEITGTSCLTFFQALDSEETQFKYVEDRFPLKLREPVARFLHFNGIRR
LDALVEKVYARFKNDFFPGEVVYLRKQKDSSTTSSNSQQSTPQPDDMVEINSVGNPGL
PQYQYQRRYVIKEKVQFNATINPESREIVMPAHTKYMLIEEAASSNKSFIVDQGQIYR
DRSTFTKHLIKCFFKITLQRASSKMGAPWCVKPEYLAMYGLTMEWPKDMLKYKEDEPV
VARRSNSANVSSPESEKNKRQSKSSGKSNTSNDASNKKETKKKRKPTEVNDSENNSSE
EDKKKGQNVTSETHSKKRKKEANEEPNTENVESVPTPANAEPQAVTITSIMDDLALPY
QHPPNIFPNLTYYNEKLECISLGSTKLSRPFDSFGKLLQAYQFLNTFGSKICLSHFSL
DQFITSLKCTDPYELKGEVVLVNIRTQTSKEQEIENNGLPMKNKAETTTEEDSENPSD
WQRNSFIRDMIMKRNSDKVEYKIVHDDPASDDILDNINHNGSALLIEVFTALLRLFIN
EEGDWSCIVVENWIIDDKGVLMERKDERGEGEAKQKRNAHGYFLQDKEKIDNLKDTLK
ENATEVQKESDAKNETNSESDSKSDSDSEERDPKLEKCLNYRNVNWIERLTKRQFNNS
YWLIILLGVLEDCRHLPMYTEFIDSFIEKIIPKDISATQLPKQLWRNFCRKLSFSDKV
NALWILVDLVSHFSPDIKAAVDDSMELCGQIRSERFKVARELKTEAAVLSNLQGDLQA
IQEKLNKTDENTPSADGADKKDDSESNSEPIDLIIIEKKQKLIEEQDKKVQALQSDKN
FLDNCLFENDLQRLKPLGLDRYGNRYFWLDHNGVPFPQYPAGMNETPKSNNSLSYHSG
RLLIQGPKASSAKFFLNVSDEQLSNWQKIRNSEGISEATREVFGISKTSSGSYNYVEN
GIEVELLDSNDRVNPLIELTPIQKKIMDETPSRLLLSPDQWYCIDKLEDLSRIMDWLD
NWGRKEHDLLRQIRPIMERIKSSLSLRDHALSLTAFTKNEEKLLKELENNEFTENELN
VDSMDVDDKNSGVKSEVDVQVDAEEKREAVIDEKLEVIADELMKLDDSSKTRNVLNRI
QELEDQRDELLEQKKSIINSQRPGARILARSERKRTKISRGNKVNKQIEILTDLVNYR
HFKAMEDVIAWKNVLANSIWGSSLRKNASGNKRSGVIETVDDKLKDIVGQTSRTVTPA
PN"
gene complement(<261648..>265859)
/gene="SNT2"
/locus_tag="YGL131C"
/db_xref="GeneID:852746"
mRNA complement(<261648..>265859)
/gene="SNT2"
/locus_tag="YGL131C"
/product="DNA-binding E3 ubiquitin-protein ligase SNT2"
/transcript_id="NM_001180996.1"
/db_xref="GeneID:852746"
CDS complement(261648..265859)
/gene="SNT2"
/locus_tag="YGL131C"
/EC_number="2.3.2.27"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006357 regulation
of transcription by RNA polymerase II [PMID:23878396]"
/experiment="EXISTENCE:direct assay:GO:0034599 cellular
response to oxidative stress [PMID:23878396]"
/experiment="EXISTENCE:direct assay:GO:0061630 ubiquitin
protein ligase activity [PMID:22570702]"
/experiment="EXISTENCE:direct assay:GO:0070211 Snt2C
complex [PMID:19040720]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:23878396]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress [PMID:23878396]"
/experiment="EXISTENCE:mutant phenotype:GO:0036205 histone
catabolic process [PMID:22570702]"
/note="Subunit of Snt2C complex, RING finger ubiquitin
ligase (E3); physically associates with Ecm5p and Rpd3p;
along with Ecm5p, recruits Rpd3p to small number of
promoters; colocalizes with Ecm5p, independently of Rpd3p,
to promoters of stress response genes upon oxidative
stress; involved in ubiquitination, degradation of excess
histones; interacts with Ubc4p; role in regulating genes
encoding amine transporters; relocalizes from nucleus to
cytoplasm upon DNA replication stress"
/codon_start=1
/product="DNA-binding E3 ubiquitin-protein ligase SNT2"
/protein_id="NP_011384.1"
/db_xref="GeneID:852746"
/db_xref="SGD:S000003099"
/translation="MPKEEDFQLPRRREAAKNVNYNEMEIDTKLVQQIQIAEKSGAKT
KGSNSQTPRNCKRTSNPASRNEKFKYQKFLHDKNTCWNFIPTLPPSFRKNSRFSNILD
LDDAMIDLKKMSLFNTESVLLSANDTIYMISEPAGEPYYVGRVVNFVSKPEFSNTIHE
AIKTTSVFPAKFFQVRMNWFYRPRDIQEHVNTFNPRLVYASLHQDICPISSYRGKCSI
FHKDEVFDVLPNEKECIIRPNIFYFDELFDRYTLKYYKVYSTDKILNKWNSKSPFLYV
LNRRFRYIYTEPKYPLENVLKKYVFHELEVNELSPADYQWDKRCQFCKEWCIQKESLS
CDECGVCAHLYCMDPPLDRKPNKDVVWTCFSCLQKQQGTKDSHVRFLEEQALELDFIR
SVRQKIEEISSKAIKENVGYNTENCWFQYLGIYSISHIGDALNDSMFFPYPFKPSRVG
VKYQWNGCNHNVPWRRNSYISANSEEERGSTKTSELAWVLDASKITTRKLSEYIEQCK
SEICPILNVRGETCNFIDVVLKNLLFTNYDTAEAFKKCKRELSRKFLKEPSFTAVEIR
KFEEAVEKFGSELRPVCEYVGTQPMSMIVRFYYNWKKTERGLTVRGKLSKLSKNKRKK
EIANHENDVETKYIDDSSFDTEKLSLAESSFQCMFCKTDYSPMWYRVTGGSDDEKIKI
RMQTGVNEKTEISEKSPAHSKKNEKLGALCIRCARMWRRYAIKWVPPLETLRKITGTC
QNSFYSAIEGIIEENNTNKFTLSPFQAHNKLLEWELVQDSELIIRQRMKVYKNPNSFV
KMKRYSMTFHTQLYKMAVRSYRKNEFHPETMQRDLELFIEDNKEVRKAIPEQKPERAK
NTKDEFPVNIIRQSPGTIKTSDTSRNRKCNDVFIEKASNNNIPKITNASNDLIEISIK
TGGSSSGSVSVDKGFKFVKFDNKTFQRLRNSLKLVNNKLPKYNEPSTKKIKMINDIAL
SNPLNEPNGASYNYTVISHSKETSVALEKYHDGNKPSKMLEKDMILKHTKNKPKNPDT
AWANNSARTFCSVCKEKFNDNDNYEVVCGNCGLTVHYFCYAIKLPKDMKKNTNLKTFK
WLCDPCSNDLNPIISTTYQCSMCPTKDYDYDRYRSQSFKICPDALKCTSLGTWVHLVC
SLFNEDIKYGNGQSMQPALNTTAVLIKHSRFTCGVCRINGGGLVKCNKCQYRYHITCA
QNSSNFKLMFEKKNMSVDTTLPCIKDVKLNDTYTLRPILICDRHDISLEGNELYPLSY
KPQHTLSYIEQYCRYYKCESDHSLVELRYFEQLRLRHGEMPGNSHDSAIKPKIYVLPF
ERTCPHCGTNKSLYWYEDIICHSCNLRSGAQELDFDSASANISNDNGLPVEITQQLME
GIEPAMFDIDISEAGTDKNTHPSSQ"
gene <266145..>267524
/gene="CEG1"
/locus_tag="YGL130W"
/db_xref="GeneID:852747"
mRNA <266145..>267524
/gene="CEG1"
/locus_tag="YGL130W"
/product="mRNA guanylyltransferase"
/transcript_id="NM_001180995.3"
/db_xref="GeneID:852747"
CDS 266145..267524
/gene="CEG1"
/locus_tag="YGL130W"
/EC_number="2.7.7.50"
/experiment="EXISTENCE:genetic interaction:GO:0031533 mRNA
capping enzyme complex [PMID:9710603]"
/experiment="EXISTENCE:genetic interaction:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:15226422]"
/experiment="EXISTENCE:mutant phenotype:GO:0004484 mRNA
guanylyltransferase activity [PMID:8718687]"
/experiment="EXISTENCE:mutant phenotype:GO:0006370
7-methylguanosine mRNA capping [PMID:8718687]"
/experiment="EXISTENCE:mutant phenotype:GO:0008033 tRNA
processing [PMID:27348091]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:15226422]"
/experiment="EXISTENCE:physical interaction:GO:0005634
nucleus [PMID:9832501]"
/experiment="EXISTENCE:physical interaction:GO:0031533
mRNA capping enzyme complex [PMID:9710603]"
/note="Guanylyltransferase involved in mRNA 5' capping;
subunit of mRNA capping enzyme, which is a heterotetramer
composed of two molecules of Ceg1p and a homodimer of
Cet1p, the mRNA 5'-triphosphatase subunit; nuclear import
of Ceg1p requires interaction with Cet1p; mammalian
capping enzyme is a single bifunctional polypeptide; human
homolog RNGTT can complement yeast ceg1 null mutant"
/codon_start=1
/product="mRNA guanylyltransferase"
/protein_id="NP_011385.3"
/db_xref="GeneID:852747"
/db_xref="SGD:S000003098"
/translation="MVLAMESRVAPEIPGLIQPGNVTQDLKMMVCKLLNSPKPTKTFP
GSQPVSFQHSDVEEKLLAHDYYVCEKTDGLRVLMFIVINPVTGEQGCFMIDRENNYYL
VNGFRFPRLPQKKKEELLETLQDGTLLDGELVIQTNPMTKLQELRYLMFDCLAINGRC
LTQSPTSSRLAHLGKEFFKPYFDLRAAYPNRCTTFPFKISMKHMDFSYQLVKVAKSLD
KLPHLSDGLIFTPVKAPYTAGGKDSLLLKWKPEQENTVDFKLILDIPMVEDPSLPKDD
RNRWYYNYDVKPVFSLYVWQGGADVNSRLKHFDQPFDRKEFEILERTYRKFAELSVSD
EEWQNLKNLEQPLNGRIVECAKNQETGAWEMLRFRDDKLNGNHTSVVQKVLESINDSV
SLEDLEEIVGDIKRCWDERRANMAGGSGRPLPSQSQNATLSTSKPVHSQPPSNDKEPK
YVDEDDWSD"
gene complement(<267723..>269075)
/gene="RSM23"
/locus_tag="YGL129C"
/gene_synonym="mS29"
/db_xref="GeneID:852748"
mRNA complement(<267723..>269075)
/gene="RSM23"
/locus_tag="YGL129C"
/gene_synonym="mS29"
/product="mitochondrial 37S ribosomal protein mS29 RSM23"
/transcript_id="NM_001180994.2"
/db_xref="GeneID:852748"
CDS complement(267723..269075)
/gene="RSM23"
/locus_tag="YGL129C"
/gene_synonym="mS29"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:11278769]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:11278769]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005763
mitochondrial small ribosomal subunit [PMID:11278769]"
/note="Mitochondrial ribosomal protein of the small
subunit; has similarity to mammalian apoptosis mediator
proteins; null mutation prevents induction of apoptosis by
overproduction of metacaspase Mca1p"
/codon_start=1
/product="mitochondrial 37S ribosomal protein mS29 RSM23"
/protein_id="NP_011386.2"
/db_xref="GeneID:852748"
/db_xref="SGD:S000003097"
/translation="MLRMSTSRFIGQRLFTTARSLQAAKPAPKGKTQGFSKKSSSVSS
YSSAKRVTPGSLYKNWTNTTHTAQLQQTAVPLALPIFNFDDISKTLNKVVSYSNKQYK
SLHHLGSFKKSQFNELFQKPVCLVREDATNSFLKKLVSHPVKKFIITGEPGVGKTVLL
SQAHAYAVDSKQIIINISYPELFLNGRNDFSYDDDLKLFIQPMYLKKLIRKILKANDP
ALLKSIELSKDYKFSNANPKNASVKPFVTLNKTKNTVLDLLSVMTHPHNRGKLMKAII
DELSVQSKVPIMFTVDNFSKVLTTAYSAYRNTENKQIYSLDLQMGKLMMDIISGETKF
ANGESSTILAISGVDRTNKTLPVALGKIPVDPYVTRYHYEPKFVELLQKGNVTEFEVP
KLNKQEVNELIDYYKQSNVLLDKDITGKKWENLIDEKYFLSGNGNPRELLKSLVLSHR
"
gene complement(<269293..>270144)
/gene="CWC23"
/locus_tag="YGL128C"
/db_xref="GeneID:852749"
mRNA complement(<269293..>270144)
/gene="CWC23"
/locus_tag="YGL128C"
/product="U2-type spliceosomal complex subunit CWC23"
/transcript_id="NM_001180993.1"
/db_xref="GeneID:852749"
CDS complement(269293..270144)
/gene="CWC23"
/locus_tag="YGL128C"
/experiment="EXISTENCE:direct assay:GO:0005684 U2-type
spliceosomal complex [PMID:11884590]"
/experiment="EXISTENCE:genetic interaction:GO:0000390
spliceosomal complex disassembly [PMID:19822657]"
/note="Component of a complex containing Cef1p; putatively
involved in pre-mRNA splicing; has similarity to E. coli
DnaJ and other DnaJ-like proteins and to S. pombe Cwf23p"
/codon_start=1
/product="U2-type spliceosomal complex subunit CWC23"
/protein_id="NP_011387.2"
/db_xref="GeneID:852749"
/db_xref="SGD:S000003096"
/translation="MPGHELEDVINQRLNLYDVLELPTPLDVHTIYDDLPQIKRKYRT
LALKYHPDKHPDNPSIIHKFHLLSTATNILTNADVRPHYDRWLIEFLRKTNDIERNKL
IQKLEESESSTIPTTTPHPDLLQIQRHGELLRKLKHFNLPYGDWKHLNTQDQENASQH
PYYDCSTLRIVLDNFLQSNNKSNCLSHLRNQVFITLSANEIYDIYFSERNNYSKDDSI
IIYTVFDTPITAQHVFRNWSSGNLIPTVKDISPLIPLHYYSDFNLETELNDDIARLVS
NEPILLD"
gene complement(<270392..>270775)
/gene="SOH1"
/locus_tag="YGL127C"
/gene_synonym="MED31"
/db_xref="GeneID:852750"
mRNA complement(<270392..>270775)
/gene="SOH1"
/locus_tag="YGL127C"
/gene_synonym="MED31"
/product="mediator complex subunit SOH1"
/transcript_id="NM_001180992.1"
/db_xref="GeneID:852750"
CDS complement(270392..270775)
/gene="SOH1"
/locus_tag="YGL127C"
/gene_synonym="MED31"
/experiment="EXISTENCE:direct assay:GO:0003713
transcription coactivator activity [PMID:19057509]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006366
transcription by RNA polymerase II [PMID:19368889]"
/experiment="EXISTENCE:direct assay:GO:0070847 core
mediator complex [PMID:15477388|PMID:15356001]"
/experiment="EXISTENCE:mutant phenotype:GO:0006310 DNA
recombination [PMID:23390378]"
/experiment="EXISTENCE:mutant phenotype:GO:0006311 meiotic
gene conversion [PMID:18069899]"
/experiment="EXISTENCE:physical interaction:GO:0006281 DNA
repair [PMID:8849885]"
/note="Subunit of the RNA polymerase II mediator complex;
associates with core polymerase subunits to form the RNA
polymerase II holoenzyme; involved in telomere
maintenance; conserved with other metazoan MED31 subunits"
/codon_start=1
/product="mediator complex subunit SOH1"
/protein_id="NP_011388.1"
/db_xref="GeneID:852750"
/db_xref="SGD:S000003095"
/translation="MSSTNGNAPATPSSDQNPLPTRFEVELEFIQSLANIQYVTYLLT
QQQIWKSPNFKNYLKYLEYWCNPPYSQCIVYPNCLFILKLLNGFMESAIVNEDGLLEG
LDELPKIIQLQGPQWMNEMVERWAN"
gene <270997..>272139
/gene="SCS3"
/locus_tag="YGL126W"
/gene_synonym="FIT2B"
/db_xref="GeneID:852751"
mRNA <270997..>272139
/gene="SCS3"
/locus_tag="YGL126W"
/gene_synonym="FIT2B"
/product="Scs3p"
/transcript_id="NM_001180991.3"
/db_xref="GeneID:852751"
CDS 270997..272139
/gene="SCS3"
/locus_tag="YGL126W"
/gene_synonym="FIT2B"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095|PMID:29526591|PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0006644
phospholipid metabolic process [PMID:7706223]"
/experiment="EXISTENCE:mutant phenotype:GO:0008654
phospholipid biosynthetic process [PMID:22927826]"
/experiment="EXISTENCE:mutant phenotype:GO:0055091
phospholipid homeostasis [PMID:33033181]"
/note="Protein required for normal ER membrane
biosynthesis in response to ER stress; ortholog of the FIT
family of proteins involved in triglyceride droplet
biosynthesis; human homolog FIT2/FITM2, a fatty acyl-CoA
diphosphatase, complements the yeast null mutant; disputed
role in the synthesis of inositol phospholipids from
inositol; required for inositol prototrophy"
/codon_start=1
/product="Scs3p"
/protein_id="NP_011389.3"
/db_xref="GeneID:852751"
/db_xref="SGD:S000003094"
/translation="MSSKWFNAIHLLVCPLTVLVGYLMNAYGYGAALQATLNKDGLVN
AMLVKKGWFWTSLVGWWCIIRYRAVPGATGRDRRHIVQSFKRYAILTVWWYVFTQGIW
FGVGPIMDLVFVYTGGHCHYDVFDDAGHVNEDFQGSVTRTNRALALIHNVLTLHGHHQ
EHRQQQLWDRSIGSIQGALQATQPKTPKNVTASAAAAINTFIHDQMHRWQGPLTTSAQ
CRRFGGHWAGGHDPSGHVFLATLMCMFLLGELRVFGRRALAHLYAQKWQLVRLVTRLF
DTGPLWTWRRCGGGSMTCGARLWRAIVEPPVTCAAALLRLTRCIACDHPVIILLTLLV
TWLWQLLLTAVASRFHTVREHMSGLLAAYIVTGLVYARDAAALRPV"
gene <272520..>274322
/gene="MET13"
/locus_tag="YGL125W"
/gene_synonym="MET11; MRPL45"
/db_xref="GeneID:852752"
mRNA <272520..>274322
/gene="MET13"
/locus_tag="YGL125W"
/gene_synonym="MET11; MRPL45"
/product="methylenetetrahydrofolate reductase (NAD(P)H)
MET13"
/transcript_id="NM_001180990.1"
/db_xref="GeneID:852752"
CDS 272520..274322
/gene="MET13"
/locus_tag="YGL125W"
/gene_synonym="MET11; MRPL45"
/EC_number="1.5.1.53"
/experiment="EXISTENCE:direct assay:GO:0004489
methylenetetrahydrofolate reductase [NAD(P)H] activity
[PMID:10600168]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:mutant phenotype:GO:0009086
methionine biosynthetic process
[PMID:10029993|PMID:10600168]"
/note="Major isozyme of methylenetetrahydrofolate
reductase; catalyzes the reduction of
5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate
in the methionine biosynthesis pathway"
/codon_start=1
/product="methylenetetrahydrofolate reductase (NAD(P)H)
MET13"
/protein_id="NP_011390.2"
/db_xref="GeneID:852752"
/db_xref="SGD:S000003093"
/translation="MKITEKLEQHRQTSGKPTYSFEYFVPKTTQGVQNLYDRMDRMYE
ASLPQFIDITWNAGGGRLSHLSTDLVATAQSVLGLETCMHLTCTNMPISMIDDALENA
YHSGCQNILALRGDPPRDAENWTPVEGGFQYAKDLIKYIKSKYGDHFAIGVAGYPECH
PELPNKDVKLDLEYLKQKIDAGGDFIITQMFYDVDNFINWCSQVRAAGMDVPIIPGIM
PITTYAAFLRRAQWGQISIPQHFSSRLDPIKDDDELVRDIGTNLIVEMCQKLLDSGYV
SHLHIYTMNLEKAPLMILERLNILPTESEFNAHPLAVLPWRKSLNPKRKNEEVRPIFW
KRRPYSYVARTSQWAVDEFPNGRFGDSSSPAFGDLDLCGSDLIRQSANKCLELWSTPT
SINDVAFLVINYLNGNLKCLPWSDIPINDEINPIKAHLIELNQHSIITINSQPQVNGI
RSNDKIHGWGPKDGYVYQKQYLEFMLPKTKLPKLIDTLKNNEFLTYFAIDSQGDLLSN
HPDNSKSNAVTWGIFPGREILQPTIVEKISFLAWKEEFYHILNEWKLNMNKYDKPHSA
QFIQSLIDDYCLVNIVDNDYISPDDQIHSILLSL"
gene complement(<274780..>276714)
/gene="MON1"
/locus_tag="YGL124C"
/gene_synonym="AUT12"
/db_xref="GeneID:852753"
mRNA complement(<274780..>276714)
/gene="MON1"
/locus_tag="YGL124C"
/gene_synonym="AUT12"
/product="guanine nucleotide exchange factor MON1"
/transcript_id="NM_001180989.2"
/db_xref="GeneID:852753"
CDS complement(274780..276714)
/gene="MON1"
/locus_tag="YGL124C"
/gene_synonym="AUT12"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:12364329]"
/experiment="EXISTENCE:direct assay:GO:0001786
phosphatidylserine binding [PMID:24413168]"
/experiment="EXISTENCE:direct assay:GO:0005085
guanyl-nucleotide exchange factor activity
[PMID:20797862|PMID:24413168]"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:20797862]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:12387888]"
/experiment="EXISTENCE:direct assay:GO:0010314
phosphatidylinositol-5-phosphate binding [PMID:24413168]"
/experiment="EXISTENCE:direct assay:GO:0032266
phosphatidylinositol-3-phosphate binding [PMID:24413168]"
/experiment="EXISTENCE:direct assay:GO:0035658 Mon1-Ccz1
complex [PMID:24413168]"
/experiment="EXISTENCE:genetic interaction:GO:0044395
protein targeting to vacuolar membrane [PMID:23979137]"
/experiment="EXISTENCE:mutant phenotype:GO:0006623 protein
targeting to vacuole [PMID:12134085]"
/experiment="EXISTENCE:mutant phenotype:GO:0006914
autophagy [PMID:12387888]"
/experiment="EXISTENCE:mutant phenotype:GO:0016236
macroautophagy [PMID:19793921]"
/experiment="EXISTENCE:mutant phenotype:GO:0032258
cytoplasm to vacuole targeting by the Cvt pathway
[PMID:19793921]"
/experiment="EXISTENCE:mutant phenotype:GO:0044395 protein
targeting to vacuolar membrane [PMID:23979137]"
/experiment="EXISTENCE:mutant phenotype:GO:0048278 vesicle
docking [PMID:14662743]"
/experiment="EXISTENCE:physical interaction:GO:0035658
Mon1-Ccz1 complex [PMID:20797862]"
/note="Subunit of heterodimeric guanine nucleotide
exchange factor (GEF); subunit of Mon1-Ccz1 GEF complex
which stimulates nucleotide exchange and activation of
Ypt7p, a Rab family GTPase involved in membrane tethering
and fusion events at late endosome and vacuole; GEF
activity is stimulated by membrane association and anionic
phospholipids; role in localizing Ypt7p to vacuolar
membrane; required for autophagy, CVT pathway, and
mitophagy; targeted to vacuole via AP-3 pathway"
/codon_start=1
/product="guanine nucleotide exchange factor MON1"
/protein_id="NP_011391.2"
/db_xref="GeneID:852753"
/db_xref="SGD:S000003092"
/translation="MNLNESYLDAEIPKGQLKHSKSGNFEGIPIVATTSEPTTSVNLD
ETFFKKAPIAMPICDDHSVSKSTSVNSLNTTSLASRRSPLQTKKLQAKNNLLSADLAK
SNDDTTRALNSPKKDFGPYLDSENDIRSRLAESIYSMETSIRGSELQRRPYVSNEIPN
VFKFSKFNSNCKLNESQTLCDKNFFIFTSAGKPIYCMHGKDEQIMSYTGLVNTVISYF
QVNGPSELKTISTLTSGKRLTFLDKSPILLMAQSERGESSNELLNQLDFLYSYILSSL
SERQLLRLFSKRENFDLRNYLESTDFENLDEICSLICNRMFPDLLLNSLQCLPFNHSS
RLKLQNVVLQQLEKRQDIPRGTLLYGLIIAPQNKLCCVLRPRGHTLHTTDLHLLFCLI
SHQFQNLDETQELWVPICFPKFNSSGFLYCYIKFLPNDTHSNEKSALVLISAQKDAFF
SLKSFSDELIIKLEEEKLLKKINTSKGFKLSDIPAPMVHHFIYKSKQNVQYVMPHFEV
NSNIALDSSQGLEYELKLKTYYQQLHGTVVRDNGNLLSRSMLNFVRWSSKDNEDLAMD
ETQMDFSELDEYIIGNSSFKQESVNMVGMAWVTPTFELYLIGNNGIVDKRVLFKSARK
VANWCQKHESRLFISDGAVF"
gene <277617..>278381
/gene="RPS2"
/locus_tag="YGL123W"
/gene_synonym="RPS4; SUP138; SUP38; SUP44"
/db_xref="GeneID:852754"
mRNA <277617..>278381
/gene="RPS2"
/locus_tag="YGL123W"
/gene_synonym="RPS4; SUP138; SUP38; SUP44"
/product="40S ribosomal protein uS5 RPS2"
/transcript_id="NM_001180988.1"
/db_xref="GeneID:852754"
CDS 277617..278381
/gene="RPS2"
/locus_tag="YGL123W"
/gene_synonym="RPS4; SUP138; SUP38; SUP44"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:2517480]"
/experiment="EXISTENCE:direct assay:GO:0022627 cytosolic
small ribosomal subunit
[PMID:3533916|PMID:2517480|PMID:385049]"
/experiment="EXISTENCE:direct assay:GO:0032040
small-subunit processome [PMID:15590835]"
/experiment="EXISTENCE:direct assay:GO:0070181 small
ribosomal subunit rRNA binding [PMID:2517480]"
/experiment="EXISTENCE:mutant phenotype:GO:0000054
ribosomal subunit export from nucleus [PMID:16246728]"
/experiment="EXISTENCE:mutant phenotype:GO:0045903
positive regulation of translational fidelity
[PMID:17545469|PMID:3522920]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:31182640]"
/note="Protein component of the small (40S) subunit;
essential for control of translational accuracy;
phosphorylation by C-terminal domain kinase I (CTDK-I)
enhances translational accuracy; N-terminal mitochondrial
avoidance segment (MAS) required for targeting to nucleus;
methylated on one or more arginine residues by Hmt1p;
required for release of methyltransferase Bud23p from
pre-40S precursor; homologous to mammalian ribosomal
protein S2 and bacterial S5"
/codon_start=1
/product="40S ribosomal protein uS5 RPS2"
/protein_id="NP_011392.1"
/db_xref="GeneID:852754"
/db_xref="SGD:S000003091"
/translation="MSAPEAQQQKRGGFGGRNRGRPNRRGPRNTEEKGWVPVTKLGRL
VKAGKITTIEEIFLHSLPVKEFQIIDTLLPGLQDEVMNIKPVQKQTRAGQRTRFKAVV
VVGDSNGHVGLGIKTAKEVAGAIRAGIIIAKLSVIPIRRGYWGTNLGQPHSLATKTTG
KCGSVTVRLIPAPRGSGIVASPAVKKLLQLAGVEDVYTQSNGKTRTLENTLKAAFVAI
GNTYGFLTPNLWAEQPLPVSPLDIYSDEASAQKKRF"
gene complement(<278946..>280523)
/gene="NAB2"
/locus_tag="YGL122C"
/db_xref="GeneID:852755"
mRNA complement(<278946..>280523)
/gene="NAB2"
/locus_tag="YGL122C"
/product="mRNA-binding protein NAB2"
/transcript_id="NM_001180987.3"
/db_xref="GeneID:852755"
CDS complement(278946..280523)
/gene="NAB2"
/locus_tag="YGL122C"
/experiment="EXISTENCE:direct assay:GO:0000049 tRNA
binding [PMID:26220998]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:8474438|PMID:11779864|PMID:7962083]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:7962083]"
/experiment="EXISTENCE:direct assay:GO:0008097 5S rRNA
binding [PMID:26220998]"
/experiment="EXISTENCE:direct assay:GO:0008143 poly(A)
binding [PMID:17630287]"
/experiment="EXISTENCE:direct assay:GO:0008312 7S RNA
binding [PMID:26220998]"
/experiment="EXISTENCE:direct assay:GO:0033204
ribonuclease P RNA binding [PMID:26220998]"
/experiment="EXISTENCE:direct assay:GO:0045945 positive
regulation of transcription by RNA polymerase III
[PMID:26220998]"
/experiment="EXISTENCE:genetic interaction:GO:1900152
negative regulation of nuclear-transcribed mRNA catabolic
process, deadenylation-dependent decay [PMID:26119729]"
/experiment="EXISTENCE:mutant phenotype:GO:0016973
poly(A)+ mRNA export from nucleus [PMID:11779864]"
/experiment="EXISTENCE:mutant phenotype:GO:0043488
regulation of mRNA stability [PMID:17636033]"
/experiment="EXISTENCE:physical interaction:GO:0043488
regulation of mRNA stability [PMID:17636033]"
/note="Nuclear Poly(A+) RNA-binding guard protein;
required for nuclear mRNA export and poly(A) tail length
control; stimulates RNA polymerase III transcription by
enhancing TFIIIB binding to promoters; dimerizes upon RNA
binding; protects mRNA against decay by nuclear exosome in
a poly(A)-tail-dependent manner; involved in forming
export-competent mRNPs in the nucleus; autoregulates mRNA
levels; NLS binds Kap104p; protein abundance increases
under DNA replication stress; related to human hnRNPs"
/codon_start=1
/product="mRNA-binding protein NAB2"
/protein_id="NP_011393.3"
/db_xref="GeneID:852755"
/db_xref="SGD:S000003090"
/translation="MSQEQYTENLKVIVAEKLAGIPNFNEDIKYVAEYIVLLIVNGGT
VESVVDELASLFDSVSRDTLANVVQTAFFALEALQQGESAENIVSKIRMMNAQSLGQS
DIAQQQQQQQQQQQPDIAQQQPQQQPQQQPQQQPQQQPQQQPQQQPQQQPQQQPQLQP
LQPQLGTQNAMQTDAPATPSPISAFSGVVNAAAPPQFAPVDNSQRFTQRGGGAVGKNR
RGGRGGNRGGRNNNSTRFNPLAKALGMAGESNMNFTPTKKEGRCRLFPHCPLGRSCPH
AHPTKVCNEYPNCPKPPGTCEFLHPNEDEELMKEMERTREEFQKRKADLLAAKRKPVQ
TGIVLCKFGALCSNPSCPFGHPTPANEDAKVIDLMWCDKNLTCDNPECRKAHSSLSKI
KEVKPISQKKAAPPPVEKSLEQCKFGTHCTNKRCKYRHARSHIMCREGANCTRIDCLF
GHPINEDCRFGVNCKNIYCLFRHPPGRVLPEKKGAAPNSNVPTNERPFALPENAIIEN
APPQTSFTHQEQDTEMN"
gene complement(<280777..>281157)
/gene="GPG1"
/locus_tag="YGL121C"
/db_xref="GeneID:852756"
mRNA complement(<280777..>281157)
/gene="GPG1"
/locus_tag="YGL121C"
/product="Gpg1p"
/transcript_id="NM_001180986.1"
/db_xref="GeneID:852756"
CDS complement(280777..281157)
/gene="GPG1"
/locus_tag="YGL121C"
/experiment="EXISTENCE:mutant phenotype:GO:0001403
invasive growth in response to glucose limitation
[PMID:12150916]"
/experiment="EXISTENCE:mutant phenotype:GO:1905907
negative regulation of amyloid fibril formation
[PMID:19129493]"
/note="Proposed gamma subunit of the heterotrimeric G
protein; interacts with the receptor Gpr1p; involved in
regulation of pseudohyphal growth; requires Gpb1p or Gpb2p
to interact with Gpa2p; overproduction causes prion
curing"
/codon_start=1
/product="Gpg1p"
/protein_id="NP_011394.1"
/db_xref="GeneID:852756"
/db_xref="SGD:S000003089"
/translation="MFYLSDIEEEASAGAEPTYNFWEVLLFSNTQENLVTVVGELHTL
TDRVVHYKIEPESREVTATTLPSLLALLLEKRNQARRLYRDVLSMKMSELDWDIDDLF
TQLQEELTRTDDTLSMYPRRRFYH"
gene complement(<281634..>283937)
/gene="PRP43"
/locus_tag="YGL120C"
/db_xref="GeneID:852757"
mRNA complement(<281634..>283937)
/gene="PRP43"
/locus_tag="YGL120C"
/product="DEAH-box ATP-dependent RNA helicase PRP43"
/transcript_id="NM_001180985.1"
/db_xref="GeneID:852757"
CDS complement(281634..283937)
/gene="PRP43"
/locus_tag="YGL120C"
/EC_number="3.6.4.13"
/experiment="EXISTENCE:direct assay:GO:0000390
spliceosomal complex disassembly [PMID:16357217]"
/experiment="EXISTENCE:direct assay:GO:0003724 RNA
helicase activity [PMID:17875666]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/experiment="EXISTENCE:genetic interaction:GO:0000390
spliceosomal complex disassembly [PMID:17875666]"
/experiment="EXISTENCE:genetic interaction:GO:0030490
maturation of SSU-rRNA [PMID:19801658]"
/experiment="EXISTENCE:mutant phenotype:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16382143]"
/experiment="EXISTENCE:mutant phenotype:GO:0000463
maturation of LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16382143]"
/experiment="EXISTENCE:mutant phenotype:GO:0000466
maturation of 5.8S rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16382143]"
/experiment="EXISTENCE:mutant phenotype:GO:0003724 RNA
helicase activity [PMID:11886864]"
/experiment="EXISTENCE:mutant phenotype:GO:0006364 rRNA
processing [PMID:16382144|PMID:16227579]"
/experiment="EXISTENCE:mutant phenotype:GO:0042273
ribosomal large subunit biogenesis [PMID:16382143]"
/experiment="EXISTENCE:mutant phenotype:GO:0071014
post-mRNA release spliceosomal complex [PMID:11886864]"
/note="RNA helicase in the DEAH-box family; functions in
both RNA polymerase I and polymerase II transcript
metabolism; catalyzes removal of U2, U5, and U6 snRNPs
from the postsplicing lariat-intron ribonucleoprotein
complex; required for efficient biogenesis of both small-
and large-subunit rRNAs; acts with Sqs1p to promote 20S to
18S rRNA processing catalyzed by endonuclease Nob1p"
/codon_start=1
/product="DEAH-box ATP-dependent RNA helicase PRP43"
/protein_id="NP_011395.1"
/db_xref="GeneID:852757"
/db_xref="SGD:S000003088"
/translation="MGSKRRFSSEHPDPVETSIPEQAAEIAEELSKQHPLPSEEPLVH
HDAGEFKGLQRHHTSAEEAQKLEDGKINPFTGREFTPKYVDILKIRRELPVHAQRDEF
LKLYQNNQIMVFVGETGSGKTTQIPQFVLFDEMPHLENTQVACTQPRRVAAMSVAQRV
AEEMDVKLGEEVGYSIRFENKTSNKTILKYMTDGMLLREAMEDHDLSRYSCIILDEAH
ERTLATDILMGLLKQVVKRRPDLKIIIMSATLDAEKFQRYFNDAPLLAVPGRTYPVEL
YYTPEFQRDYLDSAIRTVLQIHATEEAGDILLFLTGEDEIEDAVRKISLEGDQLVREE
GCGPLSVYPLYGSLPPHQQQRIFEPAPESHNGRPGRKVVISTNIAETSLTIDGIVYVV
DPGFSKQKVYNPRIRVESLLVSPISKASAQQRAGRAGRTRPGKCFRLYTEEAFQKELI
EQSYPEILRSNLSSTVLELKKLGIDDLVHFDFMDPPAPETMMRALEELNYLACLDDEG
NLTPLGRLASQFPLDPMLAVMLIGSFEFQCSQEILTIVAMLSVPNVFIRPTKDKKRAD
DAKNIFAHPDGDHITLLNVYHAFKSDEAYEYGIHKWCRDHYLNYRSLSAADNIRSQLE
RLMNRYNLELNTTDYESPKYFDNIRKALASGFFMQVAKKRSGAKGYITVKDNQDVLIH
PSTVLGHDAEWVIYNEFVLTSKNYIRTVTSVRPEWLIEIAPAYYDLSNFQKGDVKLSL
ERIKEKVDRLNELKQGKNKKKSKHSKK"
gene <284442..>285947
/gene="COQ8"
/locus_tag="YGL119W"
/gene_synonym="ABC1"
/db_xref="GeneID:852758"
mRNA <284442..>285947
/gene="COQ8"
/locus_tag="YGL119W"
/gene_synonym="ABC1"
/product="protein kinase COQ8"
/transcript_id="NM_001180984.1"
/db_xref="GeneID:852758"
CDS 284442..285947
/gene="COQ8"
/locus_tag="YGL119W"
/gene_synonym="ABC1"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:11279158|PMID:16823961|PMID:14576278|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:18801050]"
/experiment="EXISTENCE:direct assay:GO:0008289 lipid
binding [PMID:27499294]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:27499294]"
/experiment="EXISTENCE:mutant phenotype:GO:0004672 protein
kinase activity [PMID:22593570]"
/experiment="EXISTENCE:mutant phenotype:GO:0006744
ubiquinone biosynthetic process
[PMID:11279158|PMID:25498144]"
/experiment="EXISTENCE:mutant phenotype:GO:0008289 lipid
binding [PMID:27499294]"
/experiment="EXISTENCE:mutant phenotype:GO:0016887 ATP
hydrolysis activity [PMID:27499294]"
/note="ATPase required for ubiquinone biosynthesis and
respiratory growth; maintains levels of CoQ biosynthetic
proteins; binds to CoQ biosynthesis intermediates; UbiB
protein kinase-like family member that lacks canonical
protein kinase activity; similar to prokaryotic proteins
involved in ubiquinone biosynthesis; human homolog ADCK3
complements a coq8 null, is associated with CoQ and
respiratory-chain deficiencies, and is mutated in
autosomal-recessive cerebellar ataxia type 2"
/codon_start=1
/product="protein kinase COQ8"
/protein_id="NP_011396.1"
/db_xref="GeneID:852758"
/db_xref="SGD:S000003087"
/translation="MVTNMVKLRNLRRLYCSSRLLRTIQNGRISSVSSISLSKKYTTK
SAKEGEENVERKHEEEKKDTLKSSSVPTSRISRLFHYGSLAAGVGMNAAAKGISEVAK
GNSPTWKSLILSDSNIDRITNKFSKMRGVALKIGQLLSFQDEKVLPKELYEILSRVQN
SANHMPQRQLEKVMAKELGANWKTKFSKFDKIPMAAASIGQVHAAELPSGQRVVVKIQ
YPGVKESIDSDLNSLLMLLTASSLLPKGLFLDKTIANARTELKWECDYNREARALQKF
EALLKDDPAFEVPHVFPEYTTDNIITMTRMEGTEIMKLPKASQETKNFISENIMRLCL
EEIATFKYMQTDPNWANFLYNGRTKKIELLDFGASRPFAEDFILKYRKLLTYATLRDR
KGAYEMSVQLGYLTGLESQSMKDAHVDSVLTLGEPFRGDVDKSFDFKDQTVSDRIRGN
IGLMLNERLCPPPEETYSLHRKFSGIFLLCARMGASVHCAKLFKEIFAYKV"
rep_origin 285991..286081
/note="ARS714; Autonomously Replicating Sequence"
/db_xref="SGD:S000118455"
gene complement(287350..287455)
/locus_tag="YNCG0009C"
/db_xref="GeneID:852759"
tRNA complement(join(287350..287385,287420..287455))
/locus_tag="YNCG0009C"
/product="tRNA-Trp"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Tryptophan tRNA (tRNA-Trp), predicted by
tRNAscan-SE analysis"
/db_xref="GeneID:852759"
/db_xref="SGD:S000006771"
gene complement(<288014..>288451)
/locus_tag="YGL118C"
/db_xref="GeneID:852760"
mRNA complement(<288014..>288451)
/locus_tag="YGL118C"
/product="uncharacterized protein"
/transcript_id="NM_001348835.1"
/db_xref="GeneID:852760"
CDS complement(288014..288451)
/locus_tag="YGL118C"
/note="hypothetical protein; conserved among S. cerevisiae
strains; YGL118C is not an essential gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335776.1"
/db_xref="GeneID:852760"
/db_xref="SGD:S000003086"
/translation="MPPEPVWHISAVTEKFPTHGAILYISLTFSFYKKMLRVLWHILL
VYGKHAGKRKYRKVMTETNDSLYMKRNNCSGEYATLALSTRSCSFNMQQNDWVTMEGL
FPFVMVLCHLETKPMKIGIQLILQVPFMGLGIHKENKEFYLIL"
rep_origin 288451..288512
/note="ARS715; Putative replication origin; identified in
multiple array studies, not yet confirmed by plasmid-based
assay"
/db_xref="SGD:S000130165"
gene <288512..>289309
/gene="ARO5"
/locus_tag="YGL117W"
/db_xref="GeneID:852761"
mRNA <288512..>289309
/gene="ARO5"
/locus_tag="YGL117W"
/product="Aro5p"
/transcript_id="NM_001180982.3"
/db_xref="GeneID:852761"
CDS 288512..289309
/gene="ARO5"
/locus_tag="YGL117W"
/note="Protein involved in aromatic amino acid
biosynthesis; up-regulated following GCN4 induction, amino
acid starvation, rapamycin treatment in GCN4-dependent
manner; promoter contains Gcn4 control response element,
which is bound by Gcn4p in vivo"
/codon_start=1
/product="Aro5p"
/protein_id="NP_011398.3"
/db_xref="GeneID:852761"
/db_xref="SGD:S000003085"
/translation="MQPISIKDVESDQGKVYIVNALKDLVCKCLLEFVDIQIESFMYP
DDPKCFTRIFKGNKIVNEASDKDSKVRSYPSSLGVGHSALFPLIYIRQKTNSLRFLND
PKQLPTPLVDDMNAKFKGIIKVYENLIHLYHSYQTVDCNNMNQQKLLGDLVSRGNFML
DILHGYVTIASTIVRDSKDANILIDTVNRFIHDTILFHKRIIHNSNAYTEYHVMKRGM
QRNQSEETLVELEFRILDVSDVNLDNEFDDFLQHRKTSLKITHRRVI"
gene <289809..>291641
/gene="CDC20"
/locus_tag="YGL116W"
/gene_synonym="PAC5"
/db_xref="GeneID:852762"
mRNA <289809..>291641
/gene="CDC20"
/locus_tag="YGL116W"
/gene_synonym="PAC5"
/product="ubiquitin-protein transferase activating protein
CDC20"
/transcript_id="NM_001180981.1"
/db_xref="GeneID:852762"
CDS 289809..291641
/gene="CDC20"
/locus_tag="YGL116W"
/gene_synonym="PAC5"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0010997
anaphase-promoting complex binding [PMID:15916961]"
/experiment="EXISTENCE:direct assay:GO:0033597 mitotic
checkpoint complex [PMID:11726501]"
/experiment="EXISTENCE:direct assay:GO:0034399 nuclear
periphery [PMID:25817432]"
/experiment="EXISTENCE:direct assay:GO:0051321 meiotic
cell cycle [PMID:17889668]"
/experiment="EXISTENCE:direct assay:GO:1990757 ubiquitin
ligase activator activity [PMID:15060174|PMID:19362536]"
/experiment="EXISTENCE:genetic interaction:GO:1905786
positive regulation of anaphase-promoting
complex-dependent catabolic process [PMID:9334304]"
/experiment="EXISTENCE:mutant phenotype:GO:0031145
anaphase-promoting complex-dependent catabolic process
[PMID:12152084]"
/experiment="EXISTENCE:mutant phenotype:GO:0031398
positive regulation of protein ubiquitination
[PMID:9504909]"
/experiment="EXISTENCE:mutant phenotype:GO:0040020
regulation of meiotic nuclear division [PMID:10855492]"
/experiment="EXISTENCE:mutant phenotype:GO:0045842
positive regulation of mitotic metaphase/anaphase
transition [PMID:10647015]"
/experiment="EXISTENCE:mutant phenotype:GO:1905786
positive regulation of anaphase-promoting
complex-dependent catabolic process [PMID:9334304]"
/experiment="EXISTENCE:physical interaction:GO:0005680
anaphase-promoting complex [PMID:9501986]"
/experiment="EXISTENCE:physical interaction:GO:0007094
mitotic spindle assembly checkpoint signaling
[PMID:9461437]"
/experiment="EXISTENCE:physical interaction:GO:0033597
mitotic checkpoint complex [PMID:11726501|PMID:10704439]"
/note="Activator of anaphase-promoting complex/cyclosome
(APC/C); APC/C is required for metaphase/anaphase
transition; directs ubiquitination of mitotic cyclins,
Pds1p, and other anaphase inhibitors; cell-cycle
regulated; potential Cdc28p substrate; relative
distribution to the nucleus increases upon DNA replication
stress"
/codon_start=1
/product="ubiquitin-protein transferase activating protein
CDC20"
/protein_id="NP_011399.1"
/db_xref="GeneID:852762"
/db_xref="SGD:S000003084"
/translation="MPESSRDKGNAAISGNRSVLSIASPTKLNILSSDWSRNQGKVSK
NSLKRSSSLNIRNSKRPSLQASANSIYSRPKITIGAPPLIRRDSSFFKDEFDAKKDKA
TFSAYSSRSYPTIGSESVVSQTSLSQPTTSREVDEQFTVAADRYIPILQGASQNKVDP
ETLHEALPPPNASPISHLRAQTKIVFKQNVAEACGLDMNKRILQYMPEPPKCSSLRQK
SYIMKKRTHYSYQQEQKIPDLIKLRKINTNPERILDAPGFQDDFYLNLLSWSKKNVLA
IALDTALYLWNATTGDVSLLTDFENTTICSVTWSDDDCHISIGKEDGNTEIWDVETMS
LIRTMRSGLGVRIGSLSWLDTLIATGSRSGEIQINDVRIKQHIVSTWAEHTGEVCGLS
YKSDGLQLASGGNDNTVMIWDTRTSLPQFSKKTHTAAVKALSWCPYSPNILASGGGQT
DKHIHFWNSITGARVGSINTGSQVSSLHWGQSHTSTNGGMMNKEIVATGGNPENAISV
YNYETKFKVAEVVHAHEARICCSQLSPDGTTLATVGGDENLKFYKIFDPRCTGRSRED
GLMDGMLGLIGKEGCRTNDKENRSKNSSEIHTRRPSSTSQYLIR"
gene <292033..>293001
/gene="SNF4"
/locus_tag="YGL115W"
/gene_synonym="CAT3; SCI1"
/db_xref="GeneID:852763"
mRNA <292033..>293001
/gene="SNF4"
/locus_tag="YGL115W"
/gene_synonym="CAT3; SCI1"
/product="AMP-activated serine/threonine-protein kinase
regulatory subunit SNF4"
/transcript_id="NM_001180980.1"
/db_xref="GeneID:852763"
CDS 292033..293001
/gene="SNF4"
/locus_tag="YGL115W"
/gene_synonym="CAT3; SCI1"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:2481228|PMID:17237508]"
/experiment="EXISTENCE:direct assay:GO:0005641 nuclear
envelope lumen [PMID:17237508]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:2481228|PMID:17237508]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:12562756]"
/experiment="EXISTENCE:direct assay:GO:0031588
nucleotide-activated protein kinase complex
[PMID:2481228|PMID:12393914]"
/experiment="EXISTENCE:genetic interaction:GO:0004679
AMP-activated protein kinase activity [PMID:2557546]"
/experiment="EXISTENCE:genetic interaction:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:2557546]"
/experiment="EXISTENCE:genetic interaction:GO:0043539
protein serine/threonine kinase activator activity
[PMID:2557546]"
/experiment="EXISTENCE:mutant phenotype:GO:0004679
AMP-activated protein kinase activity
[PMID:7913470|PMID:11486005|PMID:2557546]"
/experiment="EXISTENCE:mutant phenotype:GO:0006914
autophagy [PMID:31962153]"
/experiment="EXISTENCE:mutant phenotype:GO:0007031
peroxisome organization [PMID:1355328]"
/experiment="EXISTENCE:mutant phenotype:GO:0043539 protein
serine/threonine kinase activator activity
[PMID:2557546|PMID:11486005]"
/experiment="EXISTENCE:mutant phenotype:GO:0045722
positive regulation of gluconeogenesis [PMID:2481228]"
/experiment="EXISTENCE:physical interaction:GO:0031588
nucleotide-activated protein kinase complex
[PMID:9121458|PMID:2481228]"
/experiment="EXISTENCE:physical interaction:GO:0045722
positive regulation of gluconeogenesis [PMID:2481228]"
/note="Activating gamma subunit of the AMP-activated Snf1p
kinase complex; additional subunits of the complex are
Snf1p and a Sip1p/Sip2p/Gal83p family member; activates
glucose-repressed genes, represses glucose-induced genes;
role in sporulation, and peroxisome biogenesis; protein
abundance increases in response to DNA replication stress"
/codon_start=1
/product="AMP-activated serine/threonine-protein kinase
regulatory subunit SNF4"
/protein_id="NP_011400.1"
/db_xref="GeneID:852763"
/db_xref="SGD:S000003083"
/translation="MKPTQDSQEKVSIEQQLAVESIRKFLNSKTSYDVLPVSYRLIVL
DTSLLVKKSLNVLLQNSIVSAPLWDSKTSRFAGLLTTTDFINVIQYYFSNPDKFELVD
KLQLDGLKDIERALGVDQLDTASIHPSRPLFEACLKMLESRSGRIPLIDQDEETHREI
VVSVLTQYRILKFVALNCRETHFLKIPIGDLNIITQDNMKSCQMTTPVIDVIQMLTQG
RVSSVPIIDENGYLINVYEAYDVLGLIKGGIYNDLSLSVGEALMRRSDDFEGVYTCTK
NDKLSTIMDNIRKARVHRFFVVDDVGRLVGVLTLSDILKYILLGSN"
gene <293460..>295637
/locus_tag="YGL114W"
/db_xref="GeneID:852764"
mRNA <293460..>295637
/locus_tag="YGL114W"
/product="uncharacterized protein"
/transcript_id="NM_001180979.1"
/db_xref="GeneID:852764"
CDS 293460..295637
/locus_tag="YGL114W"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:19001347]"
/note="hypothetical protein; predicted member of the
oligopeptide transporter (OPT) family of membrane
transporters"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011401.1"
/db_xref="GeneID:852764"
/db_xref="SGD:S000003082"
/translation="MPQSTPSQEVQRVPWDNKPALKQITLRATIAGIAIGSLVLTSNF
QFGLQTGWVSMMSLPSALLACAFFKNIWPLIFPNDRPFSDVENVYVQSMAVAVGTGPL
AFGFVGVIPAIEKFLTNDESGGLREQGQSFTFRELLIWSTALAFFGIFFAVPLRKQVI
VREKLPFPSGSATATLISVLNGTEILQEVSKSELLEMRQRRLNECPEVLQPNRDPEEA
DYLMNSSHSELGDYTATSQDGSSILSTGSENYRANIIILLKTFVVSSLYTMVSYFVPV
IRSIPVFGKYLSNNYLWNFQPSPAYIGQGIIMGLPTVSYMLIGCFLGWGVLAPLARYK
RWVPPDADVHDWEEGVQGWILWSSLSIMVADSVVAFIVVTVKSIVKFILIDDKAALLN
NIIDDTFQSMLLEEERAINSSRRNTYVDGRQDTVRLVSRDNEIEVDSKHLVRYTTVIS
GCLVSSIICIVSIIYLFGIQVIPLYAIITALILALFLSILGIRALGETDLNPVSGIGK
ISQLIFAFIIPRDRPGSVLMNVVSGGIAEASAQQAGDLMQDLKTGHLLGASPRAQFCA
QLIGACWSIILSSFMYLCYNKVYSIPSEQFRIPTAVVWIDCARLVTGKGLPDKALECS
MILGVIFAVLSLIRNTYRDYGYGWILYIPSGVAVGVGIFNSPSFTIARFIGGWASHFW
LKNHRGDLNAKTKMIVFSSGLVLGEGIFSVINMLFICLNVPHY"
gene <295932..>297938
/gene="SLD3"
/locus_tag="YGL113W"
/db_xref="GeneID:852765"
mRNA <295932..>297938
/gene="SLD3"
/locus_tag="YGL113W"
/product="Sld3p"
/transcript_id="NM_001180978.3"
/db_xref="GeneID:852765"
CDS 295932..297938
/gene="SLD3"
/locus_tag="YGL113W"
/experiment="EXISTENCE:direct assay:GO:0000775 chromosome,
centromeric region [PMID:23746350]"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:11296242]"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:11296242]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:mutant phenotype:GO:0000727
double-strand break repair via break-induced replication
[PMID:20516198]"
/experiment="EXISTENCE:mutant phenotype:GO:0006260 DNA
replication [PMID:11296242]"
/experiment="EXISTENCE:mutant phenotype:GO:0006270 DNA
replication initiation [PMID:11296242]"
/experiment="EXISTENCE:physical interaction:GO:0031261 DNA
replication preinitiation complex [PMID:11296242]"
/note="Protein involved in the initiation of DNA
replication; required for proper assembly of replication
proteins at the origins of replication; interacts with the
DDK-phosphorylated MCM complex and required for the
recruitment of Cdc45p; localizes to nuclear foci that
become diffuse upon DNA replication stress; homologous to
the human Treslin/Ticrr protein"
/codon_start=1
/product="Sld3p"
/protein_id="NP_011402.3"
/db_xref="GeneID:852765"
/db_xref="SGD:S000003081"
/translation="METWEVIASVKEATKGLDLSLDHPLIIKSEDVPSNILQLLQQKN
RRQLKHICMKSRKEYFLLEEYGPGFWVKWPYNYFNGYSLPERRTEVVTTVERERAKRE
TLKTWDELKFKELLHLWSEEPKGSCKLEKDKDLKLDMNPPDMKGESKINDYYSDPKEY
IESKYYDALFSIHTPLAYFVKSNLVRLKNTCRTKYGSDSYKIAYQAMLQKFLLSIVQF
KDRHDNRLLLEPFSSPIADEKRKNCLTKFVIQDENKNSSTIADLCVVLKSREIKLQIL
LLLEIIGLNDLDWNFRDFEKKYKLKLKKRSLNLTKKGLVRRRSKKKTSEKDKGIERIT
TSLDYCEQLDLYLDRACILDILLSSETPNPDAIEASNGTIQEHKKNILDKSKEASLVG
FINYVLIPYFNKKVPHAVEFIIQKLKGPSMRPKRALKKVNDSTNVSSPNTVETYNRLS
TSQRASRSSIINSVPSSPALRRVDANLFSRKSIASPTPELLNSRTNSNLNEFLESETR
SLKRPSQLGRTKSDLTMNHLQKRQFSVSDLSTTRVPNSSTITLKTPFSHSTINAYKTM
NNSFRRVGKRKDINETIRLHERVDSEENVQVQATPAVKKRTVTPNKKAQLQSIIESPL
NFKDDDTHEGRKNTSNITSTPTNKPPENSSKRRVRRRLFAPEST"
gene complement(<298178..>299728)
/gene="TAF6"
/locus_tag="YGL112C"
/gene_synonym="TAF60"
/db_xref="GeneID:852766"
mRNA complement(<298178..>299728)
/gene="TAF6"
/locus_tag="YGL112C"
/gene_synonym="TAF60"
/product="TATA-binding protein-associated factor TAF6"
/transcript_id="NM_001180977.1"
/db_xref="GeneID:852766"
CDS complement(298178..299728)
/gene="TAF6"
/locus_tag="YGL112C"
/gene_synonym="TAF60"
/experiment="EXISTENCE:direct assay:GO:0000124 SAGA
complex [PMID:9674426]"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:10817999]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005669
transcription factor TFIID complex
[PMID:10788514|PMID:15448131]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0006325 chromatin
organization [PMID:9674426]"
/experiment="EXISTENCE:direct assay:GO:0006366
transcription by RNA polymerase II
[PMID:15448131|PMID:12138208]"
/experiment="EXISTENCE:direct assay:GO:0046695 SLIK
(SAGA-like) complex [PMID:12446794]"
/experiment="EXISTENCE:direct assay:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:12501245]"
/experiment="EXISTENCE:mutant phenotype:GO:0051123 RNA
polymerase II preinitiation complex assembly
[PMID:12840001]"
/experiment="EXISTENCE:mutant phenotype:GO:0060090
molecular adaptor activity [PMID:9844639]"
/experiment="EXISTENCE:mutant phenotype:GO:2000144
positive regulation of DNA-templated transcription
initiation [PMID:29485702]"
/experiment="EXISTENCE:physical interaction:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:12501245]"
/note="Subunit (60 kDa) of TFIID and SAGA complexes;
involved in transcription initiation of RNA polymerase II
and in chromatin modification, similar to histone H4;
relocalizes to the cytosol in response to hypoxia"
/codon_start=1
/product="TATA-binding protein-associated factor TAF6"
/protein_id="NP_011403.1"
/db_xref="GeneID:852766"
/db_xref="SGD:S000003080"
/translation="MSTQQQSYTIWSPQDTVKDVAESLGLENINDDVLKALAMDVEYR
ILEIIEQAVKFKRHSKRDVLTTDDVSKALRVLNVEPLYGYYDGSEVNKAVSFSKVNTS
GGQSVYYLDEEEVDFDRLINEPLPQVPRLPTFTTHWLAVEGVQPAIIQNPNLNDIRVS
QPPFIRGAIVTALNDNSLQTPVTSTTASASVTDTGASQHLSNVKPGQNTEVKPLVKHV
LSKELQIYFNKVISTLTAKSQADEAAQHMKQAALTSLRTDSGLHQLVPYFIQFIAEQI
TQNLSDLQLLTTILEMIYSLLSNTSIFLDPYIHSLMPSILTLLLAKKLGGSPKDDSPQ
EIHEFLERTNALRDFAASLLDYVLKKFPQAYKSLKPRVTRTLLKTFLDINRVFGTYYG
CLKGVSVLEGESIRFFLGNLNNWARLVFNESGITLDNIEEHLNDDSNPTRTKFTKEET
QILVDTVISALLVLKKDLPDLYEGKGEKVTDEDKEKLLERCGVTIGFHILKRDDAKEL
ISAIFFGE"
gene <299978..>301369
/gene="NSA1"
/locus_tag="YGL111W"
/db_xref="GeneID:852767"
mRNA <299978..>301369
/gene="NSA1"
/locus_tag="YGL111W"
/product="ribosome biosynthesis protein NSA1"
/transcript_id="NM_001180976.1"
/db_xref="GeneID:852767"
CDS 299978..301369
/gene="NSA1"
/locus_tag="YGL111W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0030687
preribosome, large subunit precursor
[PMID:11583614|PMID:17443350|PMID:23212245]"
/experiment="EXISTENCE:direct assay:GO:1990275 preribosome
binding [PMID:39209816]"
/experiment="EXISTENCE:mutant phenotype:GO:0042273
ribosomal large subunit biogenesis [PMID:11583614]"
/note="Constituent of 66S pre-ribosomal particles;
involved in 60S ribosomal subunit biogenesis"
/codon_start=1
/product="ribosome biosynthesis protein NSA1"
/protein_id="NP_011404.1"
/db_xref="GeneID:852767"
/db_xref="SGD:S000003079"
/translation="MRLLVSCVDSGSIKEVLCNIGTDTSVQSALQPFHVAPHLAEGLK
AYVDRMWVISEDEAILARNSGVVELVKISKHLKENEALQVDPKGESKNEKSLSDDLPK
FDISEFEITSSVSDLFDDAKLESLSSKSVKRTKLVDGFVTLCPIKKDSSNNTFVAATK
SGLLHIIKKGEDKKLIKLASLGLKAPVEFLQLYDLEDTDTDKYIFAYGGEENLIKLVE
IDSSFQSLKQIWEAKNVKNDRLDMRVPVWPMALRFLEPSPGKTEKGKLNYQFAAITRW
SHLTKYSTQHGRKPFAQIDLLPNREPLSQMEVFDAKGENVVSSLGNFQSETFNELNVI
TTDYKKNVFKFDGNGRMLGKVGRDDITGSSTYIHVHDGKYLLQGGLDRYVRIFDIKTN
KMLVKVYVGSRINFIVMLDDVEIEMPLSPSAKAAKGKQKRKVTELEEDADELWNKLEG
KVAASKASKKSKI"
gene complement(<301537..>303411)
/gene="CUE3"
/locus_tag="YGL110C"
/gene_synonym="RQT3"
/db_xref="GeneID:852768"
mRNA complement(<301537..>303411)
/gene="CUE3"
/locus_tag="YGL110C"
/gene_synonym="RQT3"
/product="Cue3p"
/transcript_id="NM_001180975.1"
/db_xref="GeneID:852768"
CDS complement(301537..303411)
/gene="CUE3"
/locus_tag="YGL110C"
/gene_synonym="RQT3"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0022626 cytosolic
ribosome [PMID:36627279]"
/experiment="EXISTENCE:direct assay:GO:0043130 ubiquitin
binding [PMID:12628920]"
/experiment="EXISTENCE:direct assay:GO:0070530 K63-linked
polyubiquitin modification-dependent protein binding
[PMID:36627279]"
/experiment="EXISTENCE:direct assay:GO:0072344 rescue of
stalled cytosolic ribosome [PMID:36627279]"
/experiment="EXISTENCE:mutant phenotype:GO:0072344 rescue
of stalled cytosolic ribosome [PMID:28757607]"
/note="Subunit of ribosome-associated quality control
trigger complex (RQT); has a CUE domain that binds
ubiquitin, which may facilitate intramolecular
monoubiquitination"
/codon_start=1
/product="Cue3p"
/protein_id="NP_011405.1"
/db_xref="GeneID:852768"
/db_xref="SGD:S000003078"
/translation="MLSRYNRVIEINGGNADISLPIVKFPPFKLRAQLIEKDPVVWLH
LIETYVTYFEYLMQGANVELLDESTLDHLRLFLRTYLHEIADEEGKLLSLGINHDVSE
QLYLLKGWIFSLIKKCGLLHLQIFGDSLWNLIKVYVRRNPDSIRGLIDGSLKPRINTQ
RVQLDKSYQVQQHLKQLIESGKFKRIDLRCVEDLLSAKSMQPNKFAENFFTANWIEIL
EALWAKGQGRGHKEARELIIISLFSVSADRLLKITKELGISNFETLALYPLLGTMLIN
EGVHKRLPDLKSKLLFLNLGGLSMDEGDHMSYPTSSGTEVNEEQLSALMELFPQFSKY
QLSQTLLAYDNNIELVTNKIFEDPTIIEAFSREPAEEEVEPVSDGDNASFTEELSILD
RGDSSKNKELDKKIISEGVPDELRNKTLTRALKLLYEADEDERDDTYDEADVNRSDPS
KRIGLQEDEESYDTKDDSNEVRQDHNYHIVEAYLWNLLKEDPKLFERSKRGTKVRKTM
KEMTSWSDEKIEGWCRMLERSPTRARLLEKKFMFKGNSKTGKTSYVHNRDSQNDGNVV
KEQAKQKKSENIKKHEPQSTEQKKRQHAKNEKRKGARANHNRKKGHDKKLARAGNNAI
"
gene complement(<303649..>304071)
/locus_tag="YGL108C"
/db_xref="GeneID:852770"
mRNA complement(<303649..>304071)
/locus_tag="YGL108C"
/product="uncharacterized protein"
/transcript_id="NM_001180973.3"
/db_xref="GeneID:852770"
CDS complement(303649..304071)
/locus_tag="YGL108C"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/note="hypothetical protein, predicted to be
palmitoylated; SWAT-GFP, seamless-GFP and mCherry
C-terminal fusion proteins localize to the cytosol, while
N-terminal GFP fusion protein localizes to the cell
periphery; protein abundance increases in response to DNA
replication stress"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011407.3"
/db_xref="GeneID:852770"
/db_xref="SGD:S000003076"
/translation="MGLCGSKTQPMPSQTTTVATKARTKPINRDTVKSKQELRHKEKK
DKKKKTQLKSTTVPVVQRKEGSKLTDTSDPSKNKVSPKEAARLAAEKRFQETNEKYNK
GELGKKLAQERAKSHKTRLMEEAEKKHAERERENMIYD"
gene complement(<304333..>306273)
/gene="RMD9"
/locus_tag="YGL107C"
/db_xref="GeneID:852771"
mRNA complement(<304333..>306273)
/gene="RMD9"
/locus_tag="YGL107C"
/product="Rmd9p"
/transcript_id="NM_001180972.1"
/db_xref="GeneID:852771"
CDS complement(304333..306273)
/gene="RMD9"
/locus_tag="YGL107C"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0003730 mRNA 3'-UTR
binding [PMID:33876744]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:14562095|PMID:24769239|PMID:16823961|PMID:14576278|P
MID:17194786]"
/experiment="EXISTENCE:direct assay:GO:0090615
mitochondrial mRNA processing [PMID:33876744]"
/experiment="EXISTENCE:direct assay:GO:1903108 regulation
of mitochondrial transcription [PMID:33876744]"
/experiment="EXISTENCE:genetic interaction:GO:0006413
translational initiation [PMID:17194786]"
/experiment="EXISTENCE:mutant phenotype:GO:0009060 aerobic
respiration [PMID:17194787|PMID:17194786]"
/experiment="EXISTENCE:mutant phenotype:GO:0043488
regulation of mRNA stability [PMID:17194787]"
/note="Mitochondrial protein that controls mitochondrial
gene expression; binds the dodecamer element in
mitochondrial mRNAs, facilitates processing and delivery
of mitochondrial mRNAs to ribosomes; located on matrix
face of the inner membrane and loosely associated with
mitoribosomes; RMD9 has a paralog, YBR238C, that arose
from the whole genome duplication"
/codon_start=1
/product="Rmd9p"
/protein_id="NP_011408.1"
/db_xref="GeneID:852771"
/db_xref="SGD:S000003075"
/translation="MMLRRNAVRSLKTMEISVSNVVNSGSIAMLRGKLANVVLSDRTY
HSSPIFHKNVPKGVLDKKNGREQRKTEQNVFNVDPASPWRHELLSFDECVSSALKYST
TPLQNTYKRIGNNQLNKNPSFAMFWDSMGRAMELYYSLRESPDFNAYRVSRLIHLLHN
GLRSTRDQLVKLSRKPDYDSQSFHKEMMNFLCNSLKDISDDILIGKVSVSGYGATHLL
TSFKELSFDDDCIRIWEASKNLSDETTSQAFQEPKVVGFMLPLLYAKTRSLTEPNELY
NQIIQSKEFIHPNLYSGLIKVFIKAEDYEKALSLFGQLCEKAEVRNYGYLIETHLSFI
GDSKNLTLAESFFDKIINDEMPYKIILQVSTVNSFLQNIWKAQNDFDHVYRIWEKAVK
FYGNTVNPGILSSLNNTFFTIFFENYINDNINGFRKLQEIITFYSGVKKIDEPFFNVM
LTRASIWHERSIIDFIDKNYTLYHIPRTIISYRILLKSLGSIDNTNNEEILDRWLELV
KKLNELGQQYIANADLSALRDATVVWSQSKRDEKVFSAKAKGTPATTTTTEDDIKVPK
PLENLKNEDSTSNSEDRIELYLKILKRYTPYFRATKQVYRYTTGCAESYPILNEYLSG
YSDLSAEDIPVPQLHSFIAKEQ"
gene <306560..>307009
/gene="MLC1"
/locus_tag="YGL106W"
/db_xref="GeneID:852772"
mRNA <306560..>307009
/gene="MLC1"
/locus_tag="YGL106W"
/product="Mlc1p"
/transcript_id="NM_001180971.1"
/db_xref="GeneID:852772"
CDS 306560..307009
/gene="MLC1"
/locus_tag="YGL106W"
/experiment="EXISTENCE:direct assay:GO:0000142 cellular
bud neck contractile ring [PMID:11082046]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:15210731]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:15210731|PMID:10873803]"
/experiment="EXISTENCE:direct assay:GO:0030427 site of
polarized growth [PMID:10873803]"
/experiment="EXISTENCE:direct assay:GO:0031489 myosin V
binding [PMID:9700160]"
/experiment="EXISTENCE:direct assay:GO:0031982 vesicle
[PMID:12456647]"
/experiment="EXISTENCE:direct assay:GO:0120155 MIH complex
[PMID:24895401]"
/experiment="EXISTENCE:genetic interaction:GO:1903476
protein localization to cell division site involved in
mitotic actomyosin contractile ring assembly
[PMID:10873803]"
/experiment="EXISTENCE:mutant phenotype:GO:0006903 vesicle
targeting [PMID:12456647]"
/experiment="EXISTENCE:mutant phenotype:GO:1903476 protein
localization to cell division site involved in mitotic
actomyosin contractile ring assembly
[PMID:11082046|PMID:10873803]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding
[PMID:15210731|PMID:11082046|PMID:10873803]"
/experiment="EXISTENCE:physical interaction:GO:0016460
myosin II complex [PMID:15210731]"
/experiment="EXISTENCE:physical interaction:GO:0032038
myosin II heavy chain binding
[PMID:15210731|PMID:11082046]"
/experiment="EXISTENCE:physical interaction:GO:0120155 MIH
complex [PMID:24413167|PMID:24895401]"
/note="Essential light chain for Myo1p; light chain for
Myo2p; stabilizes Myo2p by binding to the neck region;
interacts with Myo1p, Iqg1p, and Myo2p to coordinate
formation and contraction of the actomyosin ring with
targeted membrane deposition"
/codon_start=1
/product="Mlc1p"
/protein_id="NP_011409.1"
/db_xref="GeneID:852772"
/db_xref="SGD:S000003074"
/translation="MSATRANKDIFTLFDKKGQGAIAKDSLGDYLRAIGYNPTNQLVQ
DIINADSSLRDASSLTLDQITGLIEVNEKELDATTKAKTEDFVKAFQVFDKESTGKVS
VGDLRYMLTGLGEKLTDAEVDELLKGVEVDSNGEIDYKKFIEDVLRQ"
gene <307437..>308567
/gene="ARC1"
/locus_tag="YGL105W"
/db_xref="GeneID:852773"
mRNA <307437..>308567
/gene="ARC1"
/locus_tag="YGL105W"
/product="Arc1p"
/transcript_id="NM_001180970.1"
/db_xref="GeneID:852773"
CDS 307437..308567
/gene="ARC1"
/locus_tag="YGL105W"
/experiment="EXISTENCE:direct assay:GO:0002161
aminoacyl-tRNA deacylase activity [PMID:40274265]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:15710377|PMID:11726524]"
/experiment="EXISTENCE:direct assay:GO:0008047 enzyme
activator activity [PMID:11069915]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26359986|PMID:26777405]"
/experiment="EXISTENCE:direct assay:GO:0032266
phosphatidylinositol-3-phosphate binding [PMID:16343487]"
/experiment="EXISTENCE:direct assay:GO:0080025
phosphatidylinositol-3,5-bisphosphate binding
[PMID:16343487]"
/experiment="EXISTENCE:mutant phenotype:GO:0000049 tRNA
binding [PMID:11726524]"
/experiment="EXISTENCE:mutant phenotype:GO:0006418 tRNA
aminoacylation for protein translation [PMID:9659920]"
/experiment="EXISTENCE:mutant phenotype:GO:0017102
methionyl glutamyl tRNA synthetase complex [PMID:9659920]"
/note="Protein that binds tRNA and both methionyl- and
glutamyl-tRNA synthetases; forms a complex with
methionyl-tRNA synthetase Mes1p (MetRS) and glutamyl-tRNA
synthetase Gus1p (GluRS); recruits tRNA to the complex,
increases aminoacylation efficiency by stimulating
catalysis and ensures cytoplasmic localization of the
complex; OB-fold containing protein with unexpected
aminoacyl-tRNA deacylase activity; binds quadruplex
nucleic acids; protein abundance increases in response to
DNA replication stress"
/codon_start=1
/product="Arc1p"
/protein_id="NP_011410.1"
/db_xref="GeneID:852773"
/db_xref="SGD:S000003073"
/translation="MSDLVTKFESLIISKYPVSFTKEQSAQAAQWESVLKSGQIQPHL
DQLNLVLRDNTFIVSTLYPTSTDVHVFEVALPLIKDLVASSKDVKSTYTTYRHILRWI
DYMQNLLEVSSTDKLEINHDLDLPHEVIEKKKKAPAGGAADAAAKADEDVSKKAKKQD
HPRGKPDEETLKKLREEAKAKKAAKKAANAKQQQEQQNKAPEKPKPSAIDFRVGFIQK
AIKHPDADSLYVSTIDVGDEEGPRTVCSGLVKHFPLDAMQERYVVVVCNLKPVNMRGI
KSTAMVLCGSNDDKVEFVEPPKDSKAGDKVFFEGFGDEAPMKQLNPKKKIWEHLQPHF
TTNDGLEVIFKDEEEKDHPVRKLTNAKGESFKVASIANAQVR"
gene complement(<308713..>310173)
/gene="VPS73"
/locus_tag="YGL104C"
/db_xref="GeneID:852774"
mRNA complement(<308713..>310173)
/gene="VPS73"
/locus_tag="YGL104C"
/product="putative sugar transporter"
/transcript_id="NM_001180969.3"
/db_xref="GeneID:852774"
CDS complement(308713..310173)
/gene="VPS73"
/locus_tag="YGL104C"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:mutant phenotype:GO:0006623 protein
targeting to vacuole [PMID:12134085]"
/note="Mitochondrial protein; mutation affects vacuolar
protein sorting; putative transporter; member of the sugar
porter family; VPS73 has a paralog, YBR241C, that arose
from the whole genome duplication"
/codon_start=1
/product="putative sugar transporter"
/protein_id="NP_011411.3"
/db_xref="GeneID:852774"
/db_xref="SGD:S000003072"
/translation="MNRILSSASLLSNVSMPRQNKHKITKALCYAIIVASIGSIQFGY
HLSELNAPQQVLSCSEFDIPMEGYPYDRTWLGKRGYKQCIPLNDEQIGIVTSVFCIGG
ILGSYFATSLANIYGRKFSSLINCTLNIVGSLIIFNSNSYRGLIIGRILVGISCGSLI
VIIPLFIKEVAPSGWEGLLGSMTQICIRLGVLLTQGIALPLTDSYRWRWILFGSFLIA
VLNFFMWFIVDESPKWLLAHGRVTDAKLSLCKLRGVTFDEAAQEIQDWQLQIESGDPL
IEPTTTNSISGSNSLWKYLRDRTNVKSRHVITVLLFGQQFCGINSIVLYGTKIISQLY
PQHAIRINFFISMVNVLVTILVSLLIHSLPRKPLLMTSTVLVSVTAFIMGIAMNHNKM
NLLIVFSFIYMGVFTMGLNPLPFIIMREVSKPQDMVLAQRYGTICNWVGTFIIAYTFP
IIHDVLSGYVFIIFAIIACSISAFIWKKVPETKRSG"
gene <310967..>311927
/gene="RPL28"
/locus_tag="YGL103W"
/gene_synonym="CYH2"
/db_xref="GeneID:852775"
mRNA join(<310967..311015,311527..>311927)
/gene="RPL28"
/locus_tag="YGL103W"
/gene_synonym="CYH2"
/product="60S ribosomal protein uL15 RPL28"
/transcript_id="NM_001180968.1"
/db_xref="GeneID:852775"
CDS join(310967..311015,311527..311927)
/gene="RPL28"
/locus_tag="YGL103W"
/gene_synonym="CYH2"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:22096102]"
/experiment="EXISTENCE:curator inference:GO:0003735
structural constituent of ribosome [PMID:22096102]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:6337137]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:22096102]"
/experiment="EXISTENCE:mutant phenotype:GO:0005634 nucleus
[PMID:2104804]"
/note="Ribosomal 60S subunit protein L28; homologous to
mammalian ribosomal protein L27A and bacterial L15; may
have peptidyl transferase activity; can mutate to
cycloheximide resistance"
/codon_start=1
/product="60S ribosomal protein uL15 RPL28"
/protein_id="NP_011412.1"
/db_xref="GeneID:852775"
/db_xref="SGD:S000003071"
/translation="MPSRFTKTRKHRGHVSAGKGRIGKHRKHPGGRGMAGGQHHHRIN
MDKYHPGYFGKVGMRYFHKQQAHFWKPVLNLDKLWTLIPEDKRDQYLKSASKETAPVI
DTLAAGYGKILGKGRIPNVPVIVKARFVSKLAEEKIRAAGGVVELIA"
gene <312193..>312840
/gene="YGK1"
/locus_tag="YGL101W"
/db_xref="GeneID:852777"
mRNA <312193..>312840
/gene="YGK1"
/locus_tag="YGL101W"
/product="5'-deoxynucleotidase"
/transcript_id="NM_001180966.1"
/db_xref="GeneID:852777"
CDS 312193..312840
/gene="YGK1"
/locus_tag="YGL101W"
/EC_number="3.1.3.89"
/experiment="EXISTENCE:direct assay:GO:0002953
5'-deoxynucleotidase activity [PMID:29752939]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008253
5'-nucleotidase activity [PMID:29752939]"
/experiment="EXISTENCE:direct assay:GO:0009159
deoxyribonucleoside monophosphate catabolic process
[PMID:29752939]"
/note="5'-deoxynucleotidase; non-essential gene involved
in deoxyribonucleoside monophosphate degradation;
interacts with the DNA helicase Hpr5p; YGL101W has a
paralog, YBR242W, that arose from the whole genome
duplication"
/codon_start=1
/product="5'-deoxynucleotidase"
/protein_id="NP_011414.1"
/db_xref="GeneID:852777"
/db_xref="SGD:S000003069"
/translation="MTAVNIWKPEDNIPREILAILSKPHPNYQLAFLNIIQLLKTQRR
TGWVDHGIDPCESISDHMYRMGLTTMLITDKNVDRNKCIRIALVHDFAESLVGDITPN
DPMTKEEKHRREFETVKYLCESIIRPCSESASREILDDWLAYEKQTCLEGRYVKDIDK
YEMLVQCFEYEQKYNGKKDLKQFLGAINDIKTDEVKKWTQSLLEDRQAFFDSLKE"
gene <313234..>314283
/gene="SEH1"
/locus_tag="YGL100W"
/db_xref="GeneID:852778"
mRNA <313234..>314283
/gene="SEH1"
/locus_tag="YGL100W"
/product="Seh1p"
/transcript_id="NM_001180965.1"
/db_xref="GeneID:852778"
CDS 313234..314283
/gene="SEH1"
/locus_tag="YGL100W"
/experiment="EXISTENCE:curator inference:GO:0017056
structural constituent of nuclear pore [PMID:11823431]"
/experiment="EXISTENCE:direct assay:GO:0005643 nuclear
pore [PMID:10684247]"
/experiment="EXISTENCE:direct assay:GO:0031080 nuclear
pore outer ring [PMID:11823431|PMID:18046406]"
/experiment="EXISTENCE:direct assay:GO:0035859
Seh1-associated complex [PMID:21454883]"
/experiment="EXISTENCE:mutant phenotype:GO:1904263
positive regulation of TORC1 signaling [PMID:23974112]"
/note="Subunit of the Nup84 nuclear pore and SEACAT
subcomplexes; involved in nucleocytoplasmic transport and
NPC biogenesis in the nuclear pore subcomplex; subunit of
SEACAT, a subcomplex of the SEA complex that inhibits the
TORC1 inhibitory role of SEACIT (Iml1p-Npr2p-Npr3p), a GAP
for Gtr1p in response to amino acid limitation, thereby
resulting in activation of TORC1 signaling; SEA is a
coatomer-related complex that associates dynamically with
the vacuole; human SEH1 homolog"
/codon_start=1
/product="Seh1p"
/protein_id="NP_011415.1"
/db_xref="GeneID:852778"
/db_xref="SGD:S000003068"
/translation="MQPFDSGHDDLVHDVVYDFYGRHVATCSSDQHIKVFKLDKDTSN
WELSDSWRAHDSSIVAIDWASPEYGRIIASASYDKTVKLWEEDPDQEECSGRRWNKLC
TLNDSKGSLYSVKFAPAHLGLKLACLGNDGILRLYDALEPSDLRSWTLTSEMKVLSIP
PANHLQSDFCLSWCPSRFSPEKLAVSALEQAIIYQRGKDGKLHVAAKLPGHKSLIRSI
SWAPSIGRWYQLIATGCKDGRIRIFKITEKLSPLASEESLTNSNMFDNSADVDMDAQG
RSDSNTEEKAELQSNLQVELLSEHDDHNGEVWSVSWNLTGTILSSAGDDGKVRLWKAT
YSNEFKCMSVITAQQ"
gene <314631..>316553
/gene="LSG1"
/locus_tag="YGL099W"
/gene_synonym="KRE35"
/db_xref="GeneID:852779"
mRNA <314631..>316553
/gene="LSG1"
/locus_tag="YGL099W"
/gene_synonym="KRE35"
/product="ribosome biogenesis GTPase LSG1"
/transcript_id="NM_001180964.3"
/db_xref="GeneID:852779"
CDS 314631..316553
/gene="LSG1"
/locus_tag="YGL099W"
/gene_synonym="KRE35"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:12773575]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:12773575]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807]"
/experiment="EXISTENCE:mutant phenotype:GO:0000027
ribosomal large subunit assembly [PMID:20670889]"
/experiment="EXISTENCE:mutant phenotype:GO:0000054
ribosomal subunit export from nucleus [PMID:12773575]"
/note="Putative GTPase involved in 60S ribosomal subunit
biogenesis; required for the release of Nmd3p from 60S
subunits in the cytoplasm"
/codon_start=1
/product="ribosome biogenesis GTPase LSG1"
/protein_id="NP_011416.3"
/db_xref="GeneID:852779"
/db_xref="SGD:S000003067"
/translation="MPPKEAPKKWKAPKGPKPTHRKNKNKLELGRAIKYARQKENAIE
YLPDGEMRFTTDKHEANWVKLRSVTQESALDEFLSTAALADKDFTADRHSNVKIIRMD
SGNDSATSQGFSMTNEQRGNLNAKQRALAKDLIVPRRPEWNEGMSKFQLDRQEKEAFL
EWRRKLAHLQESNEDLLLTPFERNIEVWKQLWRVVERSDLVVQIVDARNPLLFRSVDL
ERYVKESDDRKANLLLVNKADLLTKKQRIAWAKYFISKNISFTFYSALRANQLLEKQK
EMGEDYREQDFEEADKEGFDADEKVMEKVKILSIDQLEELFLSKAPNEPLLPPLPGQP
PLINIGLVGYPNVGKSSTINSLVGAKKVSVSSTPGKTKHFQTIKLSDSVMLCDCPGLV
FPNFAYNKGELVCNGVLPIDQLRDYIGPAGLVAERIPKYYIEAIYGIHIQTKSRDEGG
NGDIPTAQELLVAYARARGYMTQGYGSADEPRASRYILKDYVNGKLLYVNPPPHLEDD
TPYTREECEEFNKDLYVFDRLPDTRKEQVQNAAKAKGIDIVDLARDLNQLTFSAHTGG
DTQKEAKSVTHGGKQAALYNAAEDLDRDFFKMNNVEGRLSTPFHKVQNSSAGKRHNKK
NKSKNAKSKVFSIENN"
gene 316788..317055
/gene="SNR82"
/locus_tag="YNCG0010W"
/db_xref="GeneID:9164893"
ncRNA 316788..317055
/ncRNA_class="snoRNA"
/gene="SNR82"
/locus_tag="YNCG0010W"
/product="SNR82"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:15306656]"
/experiment="EXISTENCE:mutant phenotype:GO:0030559 rRNA
pseudouridylation guide activity
[PMID:15306656|PMID:15923376]"
/experiment="EXISTENCE:mutant phenotype:GO:0031118 rRNA
pseudouridine synthesis [PMID:15306656|PMID:15923376]"
/experiment="EXISTENCE:physical interaction:GO:0031429 box
H/ACA snoRNP complex [PMID:15923376]"
/note="H/ACA box small nucleolar RNA (snoRNA); guides
pseudouridylation of large subunit (LSU) rRNA at positions
U1110, U2349, and U2351"
/transcript_id="NR_132178.1"
/db_xref="GeneID:9164893"
/db_xref="SGD:S000028467"
gene <317342..>318079
/gene="USE1"
/locus_tag="YGL098W"
/gene_synonym="SLT1"
/db_xref="GeneID:852780"
mRNA <317342..>318079
/gene="USE1"
/locus_tag="YGL098W"
/gene_synonym="SLT1"
/product="SNAP receptor USE1"
/transcript_id="NM_001180963.1"
/db_xref="GeneID:852780"
CDS 317342..318079
/gene="USE1"
/locus_tag="YGL098W"
/gene_synonym="SLT1"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0031201 SNARE
complex [PMID:12853481]"
/experiment="EXISTENCE:direct assay:GO:0098554 cytoplasmic
side of endoplasmic reticulum membrane [PMID:12893879]"
/experiment="EXISTENCE:mutant phenotype:GO:0005484 SNAP
receptor activity [PMID:12853481]"
/experiment="EXISTENCE:mutant phenotype:GO:0006890
retrograde vesicle-mediated transport, Golgi to
endoplasmic reticulum [PMID:12893879|PMID:12853481]"
/experiment="EXISTENCE:physical interaction:GO:0005484
SNAP receptor activity [PMID:12853481]"
/note="Essential SNARE protein localized to the ER;
involved in retrograde traffic from the Golgi to the ER
and Sey1p-independent homotypic ER fusion; required for
efficient nuclear fusion during mating; forms a complex
with the SNAREs Sec22p, Sec20p and Ufe1p"
/codon_start=1
/product="SNAP receptor USE1"
/protein_id="NP_011417.1"
/db_xref="GeneID:852780"
/db_xref="SGD:S000003066"
/translation="MAETSNDPFLSYVLSSKQLTNLNRLRRKAVTKQLGSSDDNKVSE
EFLRYQHTYQREAFEYLQTKHDAHKIMESQYEQYQSSSKTRRYSIDLDSVDAVDTESQ
TEYPNEEFIDRNEDSEAVMELRKRLLGKGQNKGLGYETTKSVDRQIEDQDTLQQDLIQ
DMSKLVGSLKQGAVAFQSALDEDKQVLGAAEIGIQVASQGLMDVSGKLRKYDKSKLSY
LFYITVFIFMILGLVFTFIIIQLFPAL"
repeat_region 318697..318860
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006942"
repeat_region complement(319009..319341)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006936"
repeat_region complement(319423..319762)
/note="Ty3 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006937"
gene 319781..319852
/locus_tag="YNCG0011W"
/db_xref="GeneID:852781"
tRNA 319781..319852
/locus_tag="YNCG0011W"
/product="tRNA-His"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0030371 translation
repressor activity [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043555 regulation
of translation in response to stress [PMID:27609601]"
/note="Histidine tRNA (tRNA-His), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:852781"
/db_xref="SGD:S000006596"
repeat_region complement(320355..320707)
/note="Ty4 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006939"
gene <321782..>323230
/gene="SRM1"
/locus_tag="YGL097W"
/gene_synonym="MTR1; PRP20; TSM437"
/db_xref="GeneID:852782"
mRNA <321782..>323230
/gene="SRM1"
/locus_tag="YGL097W"
/gene_synonym="MTR1; PRP20; TSM437"
/product="Ran guanyl-nucleotide exchange factor"
/transcript_id="NM_001180962.1"
/db_xref="GeneID:852782"
CDS 321782..323230
/gene="SRM1"
/locus_tag="YGL097W"
/gene_synonym="MTR1; PRP20; TSM437"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:16365162]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:1666302|PMID:11024003|PMID:1865879]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11024003]"
/experiment="EXISTENCE:genetic interaction:GO:0005085
guanyl-nucleotide exchange factor activity [PMID:1865879]"
/experiment="EXISTENCE:mutant phenotype:GO:0000054
ribosomal subunit export from nucleus [PMID:11739405]"
/experiment="EXISTENCE:mutant phenotype:GO:0006606 protein
import into nucleus [PMID:11024003]"
/experiment="EXISTENCE:mutant phenotype:GO:0006997 nucleus
organization [PMID:8455603]"
/experiment="EXISTENCE:mutant phenotype:GO:0016973
poly(A)+ mRNA export from nucleus [PMID:27385342]"
/note="Nucleotide exchange factor for Gsp1p; localizes to
the nucleus, required for nucleocytoplasmic trafficking of
macromolecules; suppressor of the pheromone response
pathway; potentially phosphorylated by Cdc28p; human
homolog of the RAN GEF, RCC1, can complement a temperature
sensitive point mutant"
/codon_start=1
/product="Ran guanyl-nucleotide exchange factor"
/protein_id="NP_011418.1"
/db_xref="GeneID:852782"
/db_xref="SGD:S000003065"
/translation="MVKRTVATNGDASGAHRAKKMSKTHASHIINAQEDYKHMYLSVQ
PLDIFCWGTGSMCELGLGPLAKNKEVKRPRLNPFLPRDEAKIISFAVGGMHTLALDEE
SNVWSWGCNDVGALGRDTSNAKEQLKDMDADDSSDDEDGDLNELESTPAKIPRESFPP
LAEGHKVVQLAATDNMSCALFSNGEVYAWGTFRCNEGILGFYQDKIKIQKTPWKVPTF
SKYNIVQLAPGKDHILFLDEEGMVFAWGNGQQNQLGRKVMERFRLKTLDPRPFGLRHV
KYIASGENHCFALTKDNKLVSWGLNQFGQCGVSEDVEDGALVTKPKRLALPDNVVIRS
IAAGEHHSLILSQDGDLYSCGRLDMFEVGIPKDNLPEYTYKDVHGKARAVPLPTKLNN
VPKFKSVAAGSHHSVAVAQNGIAYSWGFGETYAVGLGPFEDDTEVPTRIKNTATQDHN
IILVGCGGQFSVSGGVKLSDEDAEKRADEMDD"
gene <325331..>326161
/gene="TOS8"
/locus_tag="YGL096W"
/db_xref="GeneID:852783"
mRNA <325331..>326161
/gene="TOS8"
/locus_tag="YGL096W"
/product="Tos8p"
/transcript_id="NM_001180961.1"
/db_xref="GeneID:852783"
CDS 325331..326161
/gene="TOS8"
/locus_tag="YGL096W"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:12464632]"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:12464632]"
/note="Homeodomain-containing protein and putative
transcription factor; found associated with chromatin;
target of SBF transcription factor; induced during meiosis
and under cell-damaging conditions; TOS8 has a paralog,
CUP9, that arose from the whole genome duplication"
/codon_start=1
/product="Tos8p"
/protein_id="NP_011419.1"
/db_xref="GeneID:852783"
/db_xref="SGD:S000003064"
/translation="MGTSIVNLNQKIELPPIQVLFESLNRENETKPHFEERRLYQPNP
SFVPRTNIAVGSPVNPVPVSSPVFFIGPSPQRSIQNHNAIMTQNIRQYPVIYNNNREV
ISTGERNYIITVGGPPVTSSQPEYEHISTPNFYQEQRLAQPHPVNESMMIGGYTNPQP
ISISRGKMLSGNISTNSVRGSNNGYSAKEKKHKAHGKRSNLPKATVSILNKWLHEHVN
NPYPTVQEKRELLAKTGLTKLQISNWFINARRRKIFSGQNDANNFRRKFSSSTNLAKF
"
repeat_region 327904..328224
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006943"
gene 328583..328654
/gene="SOE1"
/locus_tag="YNCG0012W"
/db_xref="GeneID:852784"
tRNA 328583..328654
/gene="SOE1"
/locus_tag="YNCG0012W"
/product="tRNA-Glu"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE
analysis; thiolation of uridine at wobble position (34)
requires Ncs6p; can mutate to become a missense suppressor
tRNA that inserts glutamate residues at lysine codons;
target of K. lactis zymocin"
/db_xref="GeneID:852784"
/db_xref="SGD:S000006553"
gene complement(<328874..>330607)
/gene="VPS45"
/locus_tag="YGL095C"
/gene_synonym="STT10; VPL28"
/db_xref="GeneID:852785"
mRNA complement(<328874..>330607)
/gene="VPS45"
/locus_tag="YGL095C"
/gene_synonym="STT10; VPL28"
/product="Vps45p"
/transcript_id="NM_001180960.3"
/db_xref="GeneID:852785"
CDS complement(328874..330607)
/gene="VPS45"
/locus_tag="YGL095C"
/gene_synonym="STT10; VPL28"
/experiment="EXISTENCE:direct assay:GO:0000139 Golgi
membrane [PMID:7720726]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:9624182]"
/experiment="EXISTENCE:genetic interaction:GO:0006895
Golgi to endosome transport [PMID:9335586]"
/experiment="EXISTENCE:mutant phenotype:GO:0000011 vacuole
inheritance [PMID:1493335]"
/experiment="EXISTENCE:mutant phenotype:GO:0006623 protein
targeting to vacuole [PMID:7628704]"
/experiment="EXISTENCE:mutant phenotype:GO:0006896 Golgi
to vacuole transport [PMID:7720726]"
/experiment="EXISTENCE:mutant phenotype:GO:0007033 vacuole
organization [PMID:9650782]"
/experiment="EXISTENCE:mutant phenotype:GO:0007035
vacuolar acidification [PMID:7628704]"
/experiment="EXISTENCE:mutant phenotype:GO:0032258
cytoplasm to vacuole targeting by the Cvt pathway
[PMID:10545112]"
/experiment="EXISTENCE:mutant phenotype:GO:0035543
positive regulation of SNARE complex assembly
[PMID:11432826]"
/experiment="EXISTENCE:mutant phenotype:GO:0048210 Golgi
vesicle fusion to target membrane [PMID:9650782]"
/experiment="EXISTENCE:mutant phenotype:GO:0051082
unfolded protein binding [PMID:11432826]"
/experiment="EXISTENCE:physical interaction:GO:0000149
SNARE binding [PMID:16769821]"
/experiment="EXISTENCE:physical interaction:GO:0031201
SNARE complex [PMID:10397773]"
/experiment="EXISTENCE:physical interaction:GO:0051082
unfolded protein binding [PMID:11432826]"
/note="Protein of the Sec1p/Munc-18 family; essential for
vacuolar protein sorting; required for the function of
Pep12p and the early endosome/late Golgi SNARE Tlg2p;
essential for fusion of Golgi-derived vesicles with the
prevacuolar compartment; mutation in human VPS45 is
associated with congenital neutropenia and primary
myelofibrosis of infancy"
/codon_start=1
/product="Vps45p"
/protein_id="NP_011420.3"
/db_xref="GeneID:852785"
/db_xref="SGD:S000003063"
/translation="MNLFDVADFYINKIVTSQSKLSVANVNEHQRIKVLLLDKNTTPT
ISLCATQSELLKHEIYLVERIENEQREVSRHLRCLVYVKPTEETLQHLLRELRNPRYG
EYQIFFSNIVSKSQLERLAESDDLEAVTKVEEIFQDFFILNQDLFSFDLQPREFLSNK
LVWSEGGLTKCTNSLVSVLLSLKIKPDIRYEGASKICERLAKEVSYEIGKNERTFFDF
PVMDSTPVLLILDRNTDPITPLLQPWTYQSMINEYIGIKRNIVDLSKVPRIDKDLEKV
TLSSKQDAFFRDTMYLNFGELGDKVKQYVTTYKDKTQTNSQINSIEDIKNFIEKYPEF
RKLSGNVAKHMAIVGELDRQLKIKNIWEISEIEQNLSAHDANEEDFSDLIKLLQNEAV
DKYYKLKLACIYSLNNQTSSDKIRQLVEILSQQLPPEDVNFFHKFKSLFSRQDKMTQS
NHDKDDILTELARRFNSRMNSKSNTAENVYMQHIPEISSLLTDLSKNALFRDRFKEID
TQGHRVIGNQQSKDIPQDVILFVIGGVTYEEARLVHDFNGTMNNRMRVVLGGTSILST
KEYMDSIRSAK"
gene complement(<331118..>334465)
/gene="PAN2"
/locus_tag="YGL094C"
/db_xref="GeneID:852786"
mRNA complement(<331118..>334465)
/gene="PAN2"
/locus_tag="YGL094C"
/product="poly(A)-specific ribonuclease"
/transcript_id="NM_001180959.1"
/db_xref="GeneID:852786"
CDS complement(331118..334465)
/gene="PAN2"
/locus_tag="YGL094C"
/EC_number="3.1.13.4"
/experiment="EXISTENCE:direct assay:GO:0000289
nuclear-transcribed mRNA poly(A) tail shortening
[PMID:8550599]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0031251 PAN complex
[PMID:8816488]"
/experiment="EXISTENCE:genetic interaction:GO:0006301 DNA
damage tolerance [PMID:11953437]"
/experiment="EXISTENCE:mutant phenotype:GO:0000289
nuclear-transcribed mRNA poly(A) tail shortening
[PMID:8550599]"
/experiment="EXISTENCE:mutant phenotype:GO:0004535
poly(A)-specific ribonuclease activity [PMID:8550599]"
/note="Catalytic subunit of the Pan2p-Pan3p
poly(A)-ribonuclease complex; complex acts to control
poly(A) tail length and regulate the stoichiometry and
activity of postreplication repair complexes"
/codon_start=1
/product="poly(A)-specific ribonuclease"
/protein_id="NP_011421.1"
/db_xref="GeneID:852786"
/db_xref="SGD:S000003062"
/translation="MNNWQHFFNNPVDLSEHLKKPYFRFDNRDKEITAISFDEKANLI
WSGDSYGCISSYDPTFQLYTRYRGHIGGNSVKDILSHRDGILSISEDSLHFANRRGVT
KLNLTSIDIAAFSELNTMCYSPHSLKNNIYCGGDNTNWGIASIDLNRGCLDSLLNYSS
KVKLMCSNNKVLSIGRQTGTVDLLDPTSNRTIKSFNAHSASISAMDLRDNTLVTVGKS
KRFYNLYADPFVNVYDLRTMRQLPPVSFSKGTTMGSGGADFVQLHPLLPTVMIVASSS
GSFDFIDLSNPTLRTQYVHPCQSIKKLCLSPNGDVLGILEADNHLDTWRRSSNNMGMF
TNTPEMLAYPDYFNDITSDGPISVDDETYPLSSVGMPYYLDKLLSAWPPVVFKSEGTI
PQLTGKSPLPSSGKLKSNLAVISSQNEKLSTQEFPLLRYDRTKYGMRNAIPDYVCLRD
IRKQITSGLETSDIQTYTSINKYEVPPAYSRLPLTSGRFGTDNFDFTPFNNTEYSGLD
PDVDNHYTNAIIQLYRFIPEMFNFVVGCLKDENFETTLLTDLGYLFDMMERSHGKICS
SSNFQASLKSLTDKRQLENGEPQEHLEEYLESLCIRESIEDFNSSESIKRNMPQKFNR
FLLSQLIKEEAQTVNHNITLNQCFGLETEIRTECSCDHYDTTVKLLPSLSISGINKTV
IKQLNKKSNGQNILPYIEYAMKNVTQKNSICPTCGKTETITQECTVKNLPSVLSLELS
LLDTEFSNIRSSKNWLTSEFYGSIIKNKAVLRSTASELKGTSHIFKYELNGYVAKITD
NNNETRLVTYVKKYNPKENCFKWLMFNDYLVVEITEEEALKMTYPWKTPEIIIYCDAE
ELRKPFFSVDTYSINYDILFRDYFANGIRDTARREYKLLTHDEAPKSGTLVAIDAEFV
SLQSELCEIDHQGIRSIIRPKRTALARISIIRGEEGELYGVPFVDDYVVNTNHIEDYL
TRYSGILPGDLDPEKSTKRLVRRNVVYRKVWLLMQLGCVFVGHGLNNDFKHININVPR
NQIRDTAIYFLQGKRYLSLRYLAYVLLGMNIQEGNHDSIEDAHTALILYKKYLHLKEK
AIFEKVLNSVYEEGRAHNFKVPETSKG"
gene <334886..>337639
/gene="SPC105"
/locus_tag="YGL093W"
/db_xref="GeneID:852787"
mRNA <334886..>337639
/gene="SPC105"
/locus_tag="YGL093W"
/product="kinetochore-microtubule binding complex subunit
SPC105"
/transcript_id="NM_001180958.1"
/db_xref="GeneID:852787"
CDS 334886..337639
/gene="SPC105"
/locus_tag="YGL093W"
/experiment="EXISTENCE:direct assay:GO:0000776 kinetochore
[PMID:27170178|PMID:19893618]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0008017 microtubule
binding [PMID:19893618]"
/experiment="EXISTENCE:direct assay:GO:0180019 Knl1/Spc105
complex [PMID:19893618|PMID:14565975]"
/experiment="EXISTENCE:mutant phenotype:GO:0007094 mitotic
spindle assembly checkpoint signaling [PMID:19893618]"
/experiment="EXISTENCE:mutant phenotype:GO:0031134 sister
chromatid biorientation [PMID:19893618]"
/experiment="EXISTENCE:mutant phenotype:GO:0034501 protein
localization to kinetochore [PMID:19893618|PMID:24402315]"
/experiment="EXISTENCE:physical interaction:GO:0034501
protein localization to kinetochore [PMID:24402315]"
/note="Subunit of a kinetochore-microtubule binding
complex; complex bridges centromeric heterochromatin and
kinetochore MAPs and motors; required for sister chromatid
bi-orientation and kinetochore binding of SAC components;
complex also includes Kre28p; modified by sumoylation"
/codon_start=1
/product="kinetochore-microtubule binding complex subunit
SPC105"
/protein_id="NP_011422.1"
/db_xref="GeneID:852787"
/db_xref="SGD:S000003061"
/translation="MNVDERSRIGGREKDAGPGKGILKQNQSSQMTSSFLENPGVRIP
TRIITKKEVLDGSNTTSRINTSNLQSMVKRRVSFAPDVTLHSFTFVPEQNNEIKEPRR
RKTSTNSPTKISSQEEPLVTSTQIDDARTEEKTAAEEDPDTSGMELTEPIVATPDSNK
ASQHDPTSMEMTEVFPRSIRQKNPDVEGESIESSQQIDDVEAVREETMELTAIHNVHD
YDSISKDTVEGEPIDLTEYESKPYVPNSVSRSTGKSSDYSVERSNDKSDLSKSENKTN
SSQPMEITDIFHADPQNPMSLHSDNNINNDGNEMELTQIQTNFDRDNHHIDESPSEKH
AFSSNKRRKLDTVSDYAASVTTPVKEAKDTSGEDNDGDLEMMEKMSPITFSDVDNKIG
TRSNDVFTIEPGTEDTGMQTATDDEEDGENVDDNGNKIVEKTRLPEIDKEGQSGIALP
TQDYTLREFINEVGVGFLDTKLIDDLDKKVNFPLNSFNFVENQRIDNVFSAFYIDIPI
LEVEAFRCKELWRSINESKDKFKDFEAQIDKSHPPLLLQEYFSSDEKMKQLMRDQLQL
VKGYSKLEAAMEWYEWRKKQLNGLELILAENLNTLKREYEKLNEEVEKVNSIRGKIRK
LNEAIKEEIRSLKNLPSDSYKPTLMNRIKIEAFKQELMEHSISLSSSNDFTQEMRSLK
LAIAKKSNDILTLRSEVASIDKKIEKRKLFTRFDLPKLRDTLKILESLTGVRFLKFSK
ATLSIAFLQLDDLRVDINLANFKNNPLSSMKVMNDSNNDDMSYHLFTMLLKNVEAEHQ
DSMLSNLFFAMKKWRPLLKYIKLLKLLFPVKITQTEEEEALLQFKDYDRRNKTAFFYV
ISLVSFAQGVFSENGQIPMKVHISTQQDYSPSREVLSDRITHKISGVLPSFTKSRIHL
EFT"
gene <337906..>341859
/gene="NUP145"
/locus_tag="YGL092W"
/gene_synonym="RAT10"
/db_xref="GeneID:852788"
mRNA <337906..>341859
/gene="NUP145"
/locus_tag="YGL092W"
/gene_synonym="RAT10"
/product="nucleocytoplasmic transporter NUP145"
/transcript_id="NM_001180957.1"
/db_xref="GeneID:852788"
CDS 337906..341859
/gene="NUP145"
/locus_tag="YGL092W"
/gene_synonym="RAT10"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:8044840]"
/experiment="EXISTENCE:direct assay:GO:0005643 nuclear
pore [PMID:10684247]"
/experiment="EXISTENCE:direct assay:GO:0031080 nuclear
pore outer ring
[PMID:18046406|PMID:11823431|PMID:9305650]"
/experiment="EXISTENCE:direct assay:GO:0044613 nuclear
pore central transport channel [PMID:18046406]"
/experiment="EXISTENCE:direct assay:GO:0045893 positive
regulation of DNA-templated transcription [PMID:15817685]"
/experiment="EXISTENCE:genetic interaction:GO:0006606
protein import into nucleus [PMID:20647373]"
/experiment="EXISTENCE:genetic interaction:GO:0006607
NLS-bearing protein import into nucleus [PMID:15039779]"
/experiment="EXISTENCE:genetic interaction:GO:0017056
structural constituent of nuclear pore [PMID:20647373]"
/experiment="EXISTENCE:genetic interaction:GO:0036228
protein localization to nuclear inner membrane
[PMID:21659568]"
/experiment="EXISTENCE:mutant phenotype:GO:0000973
post-transcriptional tethering of RNA polymerase II gene
DNA at nuclear periphery [PMID:20932479]"
/experiment="EXISTENCE:mutant phenotype:GO:0006302
double-strand break repair [PMID:16418532]"
/experiment="EXISTENCE:mutant phenotype:GO:0006409 tRNA
export from nucleus [PMID:8524308]"
/experiment="EXISTENCE:mutant phenotype:GO:0016973
poly(A)+ mRNA export from nucleus
[PMID:9133678|PMID:9305650|PMID:8044840|PMID:9001245]"
/experiment="EXISTENCE:mutant phenotype:GO:0017056
structural constituent of nuclear pore [PMID:22331846]"
/experiment="EXISTENCE:mutant phenotype:GO:0031509
subtelomeric heterochromatin formation [PMID:16418532]"
/experiment="EXISTENCE:mutant phenotype:GO:0034398
telomere tethering at nuclear periphery
[PMID:16418532|PMID:11862215|PMID:10638763]"
/experiment="EXISTENCE:mutant phenotype:GO:0046822
regulation of nucleocytoplasmic transport [PMID:23499021]"
/experiment="EXISTENCE:mutant phenotype:GO:0051664 nuclear
pore localization
[PMID:8195299|PMID:9001245|PMID:9305650|PMID:9133678]"
/experiment="EXISTENCE:physical interaction:GO:0017056
structural constituent of nuclear pore [PMID:17418788]"
/note="Essential protein with distinct roles in two
nuclear pore subcomplexes; catalyzes its own proteolytic
cleavage in vivo to generate a C-terminal fragment that is
a structural component of the Nup84p subcomplex (with
roles in NPC biogenesis and localization of genes to the
nuclear periphery), and an N-terminal fragment that is one
of several FG-nucleoporins within the NPC central core
directly responsible for nucleocytoplasmic transport;
homologous to human NUP98"
/codon_start=1
/product="nucleocytoplasmic transporter NUP145"
/protein_id="NP_011423.1"
/db_xref="GeneID:852788"
/db_xref="SGD:S000003060"
/translation="MFNKSVNSGFTFGNQNTSTPTSTPAQPSSSLQFPQKSTGLFGNV
NVNANTSTPSPSGGLFNANSNANSISQQPANNSLFGNKPAQPSGGLFGATNNTTSKSA
GSLFGNNNATANSTGSTGLFSGSNNIASSTQNGGLFGNSNNNNITSTTQNGGLFGKPT
TTPAGAGGLFGNSSSTNSTTGLFGSNNTQSSTGIFGQKPGASTTGGLFGNNGASFPRS
GETTGTMSTNPYGINISNVPMAVADMPRSITSSLSDVNGKSDAEPKPIENRRTYSFSS
SVSGNAPLPLASQSSLVSRLSTRLKATQKSTSPNEIFSPSYSKPWLNGAGSAPLVDDF
FSSKMTSLAPNENSIFPQNGFNFLSSQRADLTELRKLKIDSNRSAAKKLKLLSGTPAI
TKKHMQDEQDSSENEPIANADSVTNIDRKENRDNNLDNTYLNGKEQSNNLNKQDGENT
LQHEKSSSFGYWCSPSPEQLERLSLKQLAAVSNFVIGRRGYGCITFQHDVDLTAFTKS
FREELFGKIVIFRSSKTVEVYPDEATKPMIGHGLNVPAIITLENVYPVDKKTKKPMKD
TTKFAEFQVFDRKLRSMREMNYISYNPFGGTWTFKVNHFSIWGLVNEEDAEIDEDDLS
KQEDGGEQPLRKVRTLAQSKPSDKEVILKTDGTFGTLSGKDDSIVEEKAYEPDLSDAD
FEGIEASPKLDVSKDWVEQLILAGSSLRSVFATSKEFDGPCQNEIDLLFSECNDEIDN
AKLIMKERRFTASYTFAKFSTGSMLLTKDIVGKSGVSIKRLPTELQRKFLFDDVYLDK
EIEKVTIEARKSNPYPQISESSLLFKDALDYMEKTSSDYNLWKLSSILFDPVSYPYKT
DNDQVKMALLKKERHCRLTSWIVSQIGPEIEEKIRNSSNEIEQIFLYLLLNDVVRASK
LAIESKNGHLSVLISYLGSNDPRIRDLAELQLQKWSTGGCSIDKNISKIYKLLSGSPF
EGLFSLKELESEFSWLCLLNLTLCYGQIDEYSLESLVQSHLDKFSLPYDDPIGVIFQL
YAANENTEKLYKEVRQRTNALDVQFCWYLIQTLRFNGTRVFSKETSDEATFAFAAQLE
FAQLHGHSLFVSCFLNDDKAAEDTIKRLVMREITLLRASTNDHILNRLKIPSQLIFNA
QALKDRYEGNYLSEVQNLLLGSSYDLAEMAIVTSLGPRLLLSNNPVQNNELKTLREIL
NEFPDSERDKWSVSINVFEVYLKLVLDNVETQETIDSLISGMKIFYDQYKHCREVAAC
CNVMSQEIVSKILEKNNPSIGDSKAKLLELPLGQPEKAYLRGEFAQDLMKCTYKI"
gene complement(<342056..>343042)
/gene="NBP35"
/locus_tag="YGL091C"
/db_xref="GeneID:852789"
mRNA complement(<342056..>343042)
/gene="NBP35"
/locus_tag="YGL091C"
/product="Fe-S cluster-binding ATPase"
/transcript_id="NM_001180956.3"
/db_xref="GeneID:852789"
CDS complement(342056..343042)
/gene="NBP35"
/locus_tag="YGL091C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:8921898|PMID:15728363]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:15728363]"
/experiment="EXISTENCE:direct assay:GO:0016226 iron-sulfur
cluster assembly [PMID:17401378]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:26195633]"
/experiment="EXISTENCE:direct assay:GO:0051539 4 iron, 4
sulfur cluster binding [PMID:17401378]"
/experiment="EXISTENCE:direct assay:GO:1904564 cytosolic
[4Fe-4S] assembly scaffold complex [PMID:26195633]"
/experiment="EXISTENCE:mutant phenotype:GO:0002098 tRNA
wobble uridine modification [PMID:18755837]"
/experiment="EXISTENCE:mutant phenotype:GO:0005506 iron
ion binding [PMID:15728363]"
/experiment="EXISTENCE:mutant phenotype:GO:0016226
iron-sulfur cluster assembly [PMID:15728363]"
/experiment="EXISTENCE:mutant phenotype:GO:0016887 ATP
hydrolysis activity [PMID:8921898]"
/note="Essential cytoplasmic iron-sulfur cluster binding
protein; forms a complex with Cfd1p that is involved in
iron-sulfur protein assembly in the cytosol; similar to
P-loop NTPases"
/codon_start=1
/product="Fe-S cluster-binding ATPase"
/protein_id="NP_011424.3"
/db_xref="GeneID:852789"
/db_xref="SGD:S000003059"
/translation="MTEILPHVNDEVLPAEYELNQPEPEHCPGPESDMAGKSDACGGC
ANKEICESLPKGPDPDIPLITDNLSGIEHKILVLSGKGGVGKSTFAAMLSWALSADED
LQVGAMDLDICGPSLPHMLGCIKETVHESNSGWTPVYVTDNLATMSIQYMLPEDDSAI
IWRGSKKNLLIKKFLKDVDWDKLDYLVIDTPPGTSDEHISINKYMRESGIDGALVVTT
PQEVALLDVRKEIDFCKKAGINILGLVENMSGFVCPNCKGESQIFKATTGGGEALCKE
LGIKFLGSVPLDPRIGKSCDMGESFLDNYPDSPASSAVLNVVEALRDAVGDV"
gene <343319..>344584
/gene="LIF1"
/locus_tag="YGL090W"
/db_xref="GeneID:852790"
mRNA <343319..>344584
/gene="LIF1"
/locus_tag="YGL090W"
/product="Lif1p"
/transcript_id="NM_001180955.1"
/db_xref="GeneID:852790"
CDS 343319..344584
/gene="LIF1"
/locus_tag="YGL090W"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:10679327]"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:10679327]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11740566]"
/experiment="EXISTENCE:direct assay:GO:0006303
double-strand break repair via nonhomologous end joining
[PMID:15342630]"
/experiment="EXISTENCE:physical interaction:GO:0032807 DNA
ligase IV complex [PMID:17567543]"
/note="Component of the DNA ligase IV complex; this
complex mediates nonhomologous end joining in DNA
double-strand break repair; sumoylated by Siz1p and Nfi1p
SUMO ligases on Lys301; physically interacts with Dnl4p
and Nej1p; homologous to mammalian XRCC4 protein"
/codon_start=1
/product="Lif1p"
/protein_id="NP_011425.1"
/db_xref="GeneID:852790"
/db_xref="SGD:S000003058"
/translation="MSQLTEFISCIPVVNEEQNEEDERGLCKIQIEDGAMLETLDENS
LSGLRIEKMLVSEGTGIFSKSSFGINDLRIFTGENIDEESKKYVWYELLKMLTGHKVY
IASLDEKVVFTKWTCRMQDDEVWKVVMELESSAIIRKIAELTLHPVKKGEIDLFEMAD
KLYKDICCVNDSYRNIKESDSSNRNRVEQLARERELLDKLLETRDERTRAMMVTLLNE
KKKKIRELHEILRQNNIKLSDDDVLDSALINTEVQKPISELNSPGKRMKRRKTVVEPQ
NLQKKLKDTSRRRANRKISNQSVIKMEDDDFDDFQFFGLSKRPIITAKDKLSEKYDDI
TSFGDDTQSISFESDSSSDVQKHLVSLEDNGIQISAGRSDEDYGDISGSESETDASAG
EKKSSNHSEQSGNDREPCLQTESETDIET"
gene complement(<344791..>345153)
/gene="MF(ALPHA)2"
/locus_tag="YGL089C"
/db_xref="GeneID:852791"
mRNA complement(<344791..>345153)
/gene="MF(ALPHA)2"
/locus_tag="YGL089C"
/product="Mf(Alpha)2p"
/transcript_id="NM_001180954.1"
/db_xref="GeneID:852791"
CDS complement(344791..345153)
/gene="MF(ALPHA)2"
/locus_tag="YGL089C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0000772 mating
pheromone activity [PMID:6306574]"
/experiment="EXISTENCE:direct assay:GO:1903135 cupric ion
binding [PMID:27476515]"
/experiment="EXISTENCE:mutant phenotype:GO:0000750
pheromone-dependent signal transduction involved in
conjugation with cellular fusion [PMID:3887136]"
/experiment="EXISTENCE:mutant phenotype:GO:0000772 mating
pheromone activity [PMID:3887136]"
/note="Mating pheromone alpha-factor, made by alpha cells;
interacts with mating type a cells to induce cell cycle
arrest and other responses leading to mating; also encoded
by MF(ALPHA)1, which is more highly expressed; binds
copper(II) ions"
/codon_start=1
/product="Mf(Alpha)2p"
/protein_id="NP_011426.1"
/db_xref="GeneID:852791"
/db_xref="SGD:S000003057"
/translation="MKFISTFLTFILAAVSVTASSDEDIAQVPAEAIIGYLDFGGDHD
IAFLPFSNATASGLLFINTTIAEAAEKEQNTTLAKREAVADAWHWLNLRPGQPMYKRE
ANADAWHWLQLKPGQPMY"
gene <345836..>346201
/locus_tag="YGL088W"
/db_xref="GeneID:852792"
mRNA <345836..>346201
/locus_tag="YGL088W"
/product="uncharacterized protein"
/transcript_id="NM_001348836.1"
/db_xref="GeneID:852792"
CDS 345836..346201
/locus_tag="YGL088W"
/note="hypothetical protein; conserved across S.
cerevisiae strains; partially overlaps snR10, a snoRNA
required for preRNA processing"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335777.1"
/db_xref="GeneID:852792"
/db_xref="SGD:S000003056"
/translation="MHEVTRTYYFFLFFFLSYKRQINAAFIALFDFPLLFIYFPFLIL
VLFYNSNANLTAIRNTYSISSRLNPSGAFLTHEECGLVLQYIYYWLGLENKFIDLGCN
SLSVVCFLADLRVYLRVPG"
gene 345986..346230
/gene="SNR10"
/locus_tag="YNCG0013W"
/db_xref="GeneID:9164894"
ncRNA 345986..346230
/ncRNA_class="snoRNA"
/gene="SNR10"
/locus_tag="YNCG0013W"
/product="SNR10"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:3327689]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:8455623|PMID:3327689]"
/experiment="EXISTENCE:mutant phenotype:GO:0000447
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from
5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:3327689]"
/experiment="EXISTENCE:mutant phenotype:GO:0000472
endonucleolytic cleavage to generate mature 5'-end of
SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:3327689]"
/experiment="EXISTENCE:mutant phenotype:GO:0030559 rRNA
pseudouridylation guide activity [PMID:9160748]"
/experiment="EXISTENCE:mutant phenotype:GO:0031118 rRNA
pseudouridine synthesis [PMID:9160748]"
/experiment="EXISTENCE:physical interaction:GO:0003723 RNA
binding [PMID:3327689]"
/experiment="EXISTENCE:physical interaction:GO:0005730
nucleolus [PMID:2121740]"
/experiment="EXISTENCE:physical interaction:GO:0031429 box
H/ACA snoRNP complex [PMID:15923376|PMID:9472021]"
/note="H/ACA box small nucleolar RNA (snoRNA); essential
gene required for for preRNA processing, specifically for
cleavage at sites A1 and A2; also guides pseudouridylation
of large subunit (LSU) rRNA at position U2923"
/transcript_id="NR_132179.1"
/db_xref="GeneID:9164894"
/db_xref="SGD:S000007499"
gene complement(<346406..>346904)
/gene="MMS2"
/locus_tag="YGL087C"
/db_xref="GeneID:852793"
mRNA complement(join(<346406..346808,346894..>346904))
/gene="MMS2"
/locus_tag="YGL087C"
/product="E2 ubiquitin-conjugating protein MMS2"
/transcript_id="NM_001180952.1"
/db_xref="GeneID:852793"
CDS complement(join(346406..346808,346894..346904))
/gene="MMS2"
/locus_tag="YGL087C"
/experiment="EXISTENCE:direct assay:GO:0000209 protein
polyubiquitination [PMID:10089880]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:32265276]"
/experiment="EXISTENCE:direct assay:GO:0004842
ubiquitin-protein transferase activity [PMID:10089880]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10880451]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:10880451]"
/experiment="EXISTENCE:direct assay:GO:0010994 free
ubiquitin chain polymerization [PMID:19706603]"
/experiment="EXISTENCE:genetic interaction:GO:0006301 DNA
damage tolerance [PMID:9576943|PMID:10089880]"
/experiment="EXISTENCE:mutant phenotype:GO:0006301 DNA
damage tolerance [PMID:9576943|PMID:10089880]"
/experiment="EXISTENCE:mutant phenotype:GO:0044395 protein
targeting to vacuolar membrane [PMID:32265276]"
/experiment="EXISTENCE:physical interaction:GO:0000209
protein polyubiquitination [PMID:10089880]"
/experiment="EXISTENCE:physical interaction:GO:0031371
ubiquitin conjugating enzyme complex [PMID:10089880]"
/note="Ubiquitin-conjugating enzyme variant; involved in
error-free postreplication repair; forms a heteromeric
complex with Ubc13p, an active ubiquitin-conjugating
enzyme; cooperates with chromatin-associated RING finger
proteins, Rad18p and Rad5p; protein abundance increases in
response to DNA replication stress"
/codon_start=1
/product="E2 ubiquitin-conjugating protein MMS2"
/protein_id="NP_011428.1"
/db_xref="GeneID:852793"
/db_xref="SGD:S000003055"
/translation="MSKVPRNFRLLEELEKGEKGFGPESCSYGLADSDDITMTKWNGT
ILGPPHSNHENRIYSLSIDCGPNYPDSPPKVTFISKINLPCVNPTTGEVQTDFHTLRD
WKRAYTMETLLLDLRKEMATPANKKLRQPKEGETF"
gene <347119..>349368
/gene="MAD1"
/locus_tag="YGL086W"
/db_xref="GeneID:852794"
mRNA <347119..>349368
/gene="MAD1"
/locus_tag="YGL086W"
/product="coiled-coil domain-containing protein MAD1"
/transcript_id="NM_001180951.3"
/db_xref="GeneID:852794"
CDS 347119..349368
/gene="MAD1"
/locus_tag="YGL086W"
/experiment="EXISTENCE:direct assay:GO:0000776 kinetochore
[PMID:19139260|PMID:24402315|PMID:27170178]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:7593191]"
/experiment="EXISTENCE:genetic interaction:GO:0006913
nucleocytoplasmic transport [PMID:12473689]"
/experiment="EXISTENCE:genetic interaction:GO:0044774
mitotic DNA integrity checkpoint signaling
[PMID:16651657]"
/experiment="EXISTENCE:mutant phenotype:GO:0000776
kinetochore [PMID:19139260]"
/experiment="EXISTENCE:mutant phenotype:GO:0007094 mitotic
spindle assembly checkpoint signaling [PMID:10704439]"
/experiment="EXISTENCE:mutant phenotype:GO:0090268
activation of mitotic cell cycle spindle assembly
checkpoint [PMID:15802513]"
/experiment="EXISTENCE:mutant phenotype:GO:1901925
negative regulation of protein import into nucleus during
spindle assembly checkpoint [PMID:23177738]"
/experiment="EXISTENCE:physical interaction:GO:0006913
nucleocytoplasmic transport [PMID:12473689]"
/note="Coiled-coil protein involved in spindle-assembly
checkpoint; required for inhibition of
karyopherin/importin Pse1p (aka Kap121p) upon spindle
assembly checkpoint arrest; phosphorylated by Mps1p upon
checkpoint activation which leads to inhibition of
anaphase promoting complex activity; forms a complex with
Mad2p; gene dosage imbalance between MAD1 and MAD2 leads
to chromosome instability"
/codon_start=1
/product="coiled-coil domain-containing protein MAD1"
/protein_id="NP_011429.3"
/db_xref="GeneID:852794"
/db_xref="SGD:S000003054"
/translation="MDVRAALQCFFSALSGRFTGKKLGLEIYSIQYKMSNSGGSSPFL
ESPGGSPDVGSTNGQSNRQIQALQFKLNTLQNEYEIEKLQLQKQTNILEKKYKATIDE
LEKALNDTKYLYESNDKLEQELKSLKERSANSMNDKDKCIEELRTTLQNKDLEMETLR
QQYDSKLSKVTNQCDHFKLEAESSHSLLMKYEKEIKRQSVDIKDLQHQVMEKDDELSS
VKASKMINSHPNYSTEEFNELTEMNKMIQDQVQYTKELELANMQQANELKKLKQSQDT
STFWKLENEKLQNKLSQLHVLESQYENLQLENIDLKSKLTKWEIYNDSDDDDDNNVNN
NDNNNNNKNDNNNDNNNDTSNNNNINNNNRTKNNIRNNPEEIIRDWKLTKKECLILTD
MNDKLRLDNNNLKLLNDEMALERNQILDLNKNYENNIVNLKRLNHELEQQKSLSFEEC
RLLREQLDGLYSAQNNALLEVENSETHASNKNVNEDMNNLIDTYKNKTEDLTNELKKL
NDQLLSNSNDVETQRKKRKLTSDQIGLNYSQRLNELQLENVSVSRELSKAQTTIQLLQ
EKLEKLTKLKEKKIRILQLRDGPFIKDQFIKKNKLLLLEKENADLLNELKKNNPAVET
VPISVYDSLNFELKQFEQEVFKSNKRFSRLKQVFNNKSLEFIDVVNSLLGFKLEFQQD
SRVKIFSCFKPEKYLIADLNENTLKSNLDADIEGWDDLMNLWVEDRGQLPCFLATITL
RLWEQRQAK"
gene <349616..>350440
/gene="LCL3"
/locus_tag="YGL085W"
/db_xref="GeneID:852795"
mRNA <349616..>350440
/gene="LCL3"
/locus_tag="YGL085W"
/product="Lcl3p"
/transcript_id="NM_001180950.1"
/db_xref="GeneID:852795"
CDS 349616..350440
/gene="LCL3"
/locus_tag="YGL085W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095]"
/note="hypothetical protein; mutant has long chronological
lifespan; has homology to Staphylococcus aureus nuclease;
GFP-fusion protein localizes to mitochondria; is induced
in response to the DNA-damaging agent MMS"
/codon_start=1
/product="Lcl3p"
/protein_id="NP_011430.1"
/db_xref="GeneID:852795"
/db_xref="SGD:S000003053"
/translation="MREGDSNSKKSADVAVLSIILTGSTLTLIYTYKRYLTQFKRTND
IPRRIFRKHWLYGKVTSVGDGDNFHFFHMPGGIRGGWGWLRPVPQMIKNDSTAEKLVG
DSRNMRFFNFNWITHGRSTKSKIQKAKSQFLKLNVPYKNRKNLPTIPIRLCGIDAPER
AHFGNPAQPFGNEALIWLQNRILGKKVWVKPLSIDQYNRCVARVSYWDWFGGWKDLSL
EMLKDGLAVVYEGKVNTEFDDREDKYRYYEFLARSRKKGLWIQNKFETPGEYKKRI"
gene complement(<350616..>352298)
/gene="GUP1"
/locus_tag="YGL084C"
/db_xref="GeneID:852796"
mRNA complement(<350616..>352298)
/gene="GUP1"
/locus_tag="YGL084C"
/product="O-acyltransferase"
/transcript_id="NM_001180949.1"
/db_xref="GeneID:852796"
CDS complement(350616..352298)
/gene="GUP1"
/locus_tag="YGL084C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:15813700|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:15813700]"
/experiment="EXISTENCE:mutant phenotype:GO:0006506 GPI
anchor biosynthetic process [PMID:16597698]"
/experiment="EXISTENCE:mutant phenotype:GO:0008374
O-acyltransferase activity [PMID:16597698|PMID:18036137]"
/experiment="EXISTENCE:mutant phenotype:GO:0015793
glycerol transmembrane transport [PMID:10931309]"
/experiment="EXISTENCE:mutant phenotype:GO:0019563
glycerol catabolic process [PMID:10747858]"
/note="Plasma membrane protein involved in remodeling GPI
anchors; member of the MBOAT family of putative
membrane-bound O-acyltransferases; role in misfolded
protein quality control; proposed to be involved in
glycerol transport; homolog of the mammalian Hedgehog
pathway modulator HHATL; GUP1 has a paralog, GUP2, that
arose from the whole genome duplication"
/codon_start=1
/product="O-acyltransferase"
/protein_id="NP_011431.1"
/db_xref="GeneID:852796"
/db_xref="SGD:S000003052"
/translation="MSLISILSPLITSEGLDSRIKPSPKKDASTTTKPSLWKTTEFKF
YYIAFLVVVPLMFYAGLQASSPENPNYARYERLLSQGWLFGRKVDNSDSQYRFFRDNF
ALLSVLMLVHTSIKRIVLYSTNITKLRFDLIFGLIFLVAAHGVNSIRILAHMLILYAI
AHVLKNFRRIATISIWIYGISTLFINDNFRAYPFGNICSFLSPLDHWYRGIIPRWDVF
FNFTLLRVLSYNLDFLERWENLQKKKSPSYESKEAKSAILLNERARLTAAHPIQDYSL
MNYIAYVTYTPLFIAGPIITFNDYVYQSKHTLPSINFKFIFYYAVRFVIALLSMEFIL
HFLHVVAISKTKAWENDTPFQISMIGLFNLNIIWLKLLIPWRLFRLWALLDGIDTPEN
MIRCVDNNYSSLAFWRAWHRSYNKWVVRYIYIPLGGSKNRVLTSLAVFSFVAIWHDIE
LKLLLWGWLIVLFLLPEIFATQIFSHYTDAVWYRHVCAVGAVFNIWVMMIANLFGFCL
GSDGTKKLLSDMFCTVSGFKFVILASVSLFIAVQIMFEIREEEKRHGIYLKC"
rep_origin 352760..352906
/note="ARS716; Autonomously Replicating Sequence"
/db_xref="SGD:S000118456"
gene <353058..>355472
/gene="SCY1"
/locus_tag="YGL083W"
/db_xref="GeneID:852797"
mRNA <353058..>355472
/gene="SCY1"
/locus_tag="YGL083W"
/product="Scy1p"
/transcript_id="NM_001180948.1"
/db_xref="GeneID:852797"
CDS 353058..355472
/gene="SCY1"
/locus_tag="YGL083W"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:38126114]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/note="Putative kinase; suppressor of GTPase mutant;
dosage-sensitive modulator of mitochondrial DNA (mtDNA)
homeostasis; localizes to the mitochondrial matrix, and to
the cytosol and clathrin-coated vesicles in HTP studies;
similar to bovine rhodopsin kinase; may have a role in
intracellular sterol transport"
/codon_start=1
/product="Scy1p"
/protein_id="NP_011432.1"
/db_xref="GeneID:852797"
/db_xref="SGD:S000003051"
/translation="MMFWSSKTGITSKYSFSSSPTFTAEPWSIYTGRPKSSSSSSPSK
VSIFMFDKKQFENYLLHYGIIKSKSGSRDKVLIQEAYEILRNQANNLAKLKHPNILTL
IEPLEEHSKNFMFVTEFVTSSLETVFRETDDEEQNFLQGHVKDNIVVQRGILQLVNAL
DFVHNRASFVHLNIQPRAIFINENSDWKISGLGYLVKIPPGTNTSEYFLPQYDPRVPP
FMHLQLNYTAPEIVFENTLTFKNDYFSLGLLIYFLYTGKDLFRSENSTSEYKLEYNKF
ESKISTMSWDNIFSKVPQKLRHCIPKLINRDIYSRYDNITLILDSEFFQDPLVKTLNF
LDDLPTKNNEEKYVFLEGLVNLLPEFPPALLQKKFLPILLELLSQFCAEKVVSDKCVG
KSLDLIIKIGSTLSQLSFQEKVYPVLLSDANFPVLLKKATICLIDNLDTLKQKVKRSD
FLENILKPLFNYVLHDSESDITVVCQEKLLSQIPLALEVLDFPTVKQFLLPLLSNLFT
KTTSLTVKNTCVTCFQIMIEHKSIDSYTCSETVLPLFKSMKTRDPRILSKLLKLFETV
PLIITDEIVLVDQVLPLMWNYSMASTLTKSQYSGYTKAINKMSSDIQKHHIAKLDDKV
NDIGEDAFHKVIEPTIMKKEDPETVAAKNIEVAAMQPVKKKTGSSYGETLPQSKSILN
SKPLNPKNALATRGFPTRILNSPPQTPSSRTGSKVMTKGGSNDASSTKVEEEFNEFQS
FSSTGSIRQTSAPSDVWMNSTPSPTPTSASSTNLPPGFSISLQPNKRKDGSSDIPRSN
VYGSLI"
gene <355827..>356972
/gene="MIY3"
/locus_tag="YGL082W"
/db_xref="GeneID:852798"
mRNA <355827..>356972
/gene="MIY3"
/locus_tag="YGL082W"
/product="Miy3p"
/transcript_id="NM_001180947.3"
/db_xref="GeneID:852798"
CDS 355827..356972
/gene="MIY3"
/locus_tag="YGL082W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836|PMID:39798877]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="hypothetical protein; MINDY family deubiquitinase
that does not appear to contain catalytic activity;
predicted prenylation/proteolysis target of Afc1p and
Rce1p; green fluorescent protein (GFP)-fusion protein
localizes to the plasma membrane; not an essential gene;
YGL082W has a paralog, YPL191C, that arose from the whole
genome duplication; ortholog of human MINDY1/FAM63A"
/codon_start=1
/product="Miy3p"
/protein_id="NP_011433.3"
/db_xref="GeneID:852798"
/db_xref="SGD:S000003050"
/translation="MDVTFLTKNVQINGTQFKILLQNGQGECALIALANVLLISPAHA
RYAQEISRLVRGKETVTLNELVQTLADMGVQNPNGTDVDKQQLLQILPQLYSGLNINP
EFNGSFEDGVEMSIFRLYNVGIVHGWIIDGDNDPNSYEHVSKYSYMGAQKVLVQSYEI
QKNNAQFENSEQIQSDAPYLKSFLARSATQLTEYGLTHLREILVERSYAVLFRNDHFC
TLYKNNGELFTLVTDPTYRNRKDINWQSLKSVNGSQDSYYTGNFIPTSLERTETTATG
QNESYISNPFSDQNTGHVTSNQVNSGASGVQQIEDDEELARRLQEQEDMRAANNMQNG
YANNGRNHQRERFERPEKNSKKNKFLPFNGSNKEKKRDKLKKNCVIM"
gene <357377..>358339
/locus_tag="YGL081W"
/db_xref="GeneID:852799"
mRNA <357377..>358339
/locus_tag="YGL081W"
/product="uncharacterized protein"
/transcript_id="NM_001180946.1"
/db_xref="GeneID:852799"
CDS 357377..358339
/locus_tag="YGL081W"
/note="hypothetical protein; contains a forkhead
associated (FHA) domain; null mutant is sensitive to UV
light; null mutant is sensitive to DNA damaging agents in
HTP studies; proposed role in the DNA damage response;
interacts genetically with CHS5, a gene involved in chitin
biosynthesis"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011434.1"
/db_xref="GeneID:852799"
/db_xref="SGD:S000003049"
/translation="MGDIRTFVFAIEDTETTQGLCKTIGRSSSFDQNSLCKPYNLYFD
EPELSRQHAVLCIKTPIPKIEGVPSIEQLRICIRDLNNKTGTVNLVSDGPNDEIDLKN
GDAFGLIAIDNHPFRDNHHLAAKLIFRIELEYFDEAREIVKCTITNVTFGKNNTVSSF
PIHSATSTEDSDSSWYGLSEASTQTEVADECHETNTILTRGGRFSILSLRKRGSKQDQ
KICSNFDRKIHETSSFEEEIEVCTDTDTTEEKEEEEEKEEGDDEEGEIELEIIRVKRI
KGRTKIKKTLTCFSKNKKIITPQHSNSMWLLLIVILIFDRLLSN"
gene <358636..>359028
/gene="MPC1"
/locus_tag="YGL080W"
/gene_synonym="FMP37"
/db_xref="GeneID:852800"
mRNA <358636..>359028
/gene="MPC1"
/locus_tag="YGL080W"
/gene_synonym="FMP37"
/product="pyruvate transporter MPC1"
/transcript_id="NM_001180945.1"
/db_xref="GeneID:852800"
CDS 358636..359028
/gene="MPC1"
/locus_tag="YGL080W"
/gene_synonym="FMP37"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane
[PMID:22628554|PMID:22628558]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:40012703]"
/experiment="EXISTENCE:direct assay:GO:0006850 pyruvate
import into mitochondria [PMID:25672363]"
/experiment="EXISTENCE:direct assay:GO:0050833 pyruvate
transmembrane transporter activity [PMID:25672363]"
/experiment="EXISTENCE:mutant phenotype:GO:0006850
pyruvate import into mitochondria
[PMID:22628558|PMID:22628554]"
/experiment="EXISTENCE:mutant phenotype:GO:0050833
pyruvate transmembrane transporter activity
[PMID:22628554|PMID:22628558]"
/note="Highly conserved subunit of mitochondrial pyruvate
carrier (MPC); MPC is a mitochondrial inner membrane
complex that mediates pyruvate uptake comprised of Mpc1p
and Mpc2p during fermentative growth, or Mpc1p and Mpc3p
during respiratory growth; null mutant displays slow
growth that is complemented by expression of the human or
mouse ortholog; mutation in human ortholog MPC1 is
associated with lactic acidosis and hyperpyruvatemia"
/codon_start=1
/product="pyruvate transporter MPC1"
/protein_id="NP_011435.1"
/db_xref="GeneID:852800"
/db_xref="SGD:S000003048"
/translation="MSQPVQRAAARSFLQKYINKETLKYIFTTHFWGPVSNFGIPIAA
IYDLKKDPTLISGPMTFALVTYSGVFMKYALSVSPKNYLLFGCHLINETAQLAQGYRF
LKYTYFTTDEEKKALDKEWKEKEKTGKQ"
gene <359444..>360100
/gene="KXD1"
/locus_tag="YGL079W"
/db_xref="GeneID:852801"
mRNA <359444..>360100
/gene="KXD1"
/locus_tag="YGL079W"
/product="Kxd1p"
/transcript_id="NM_001180944.1"
/db_xref="GeneID:852801"
CDS 359444..360100
/gene="KXD1"
/locus_tag="YGL079W"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0031083 BLOC-1
complex [PMID:23547030]"
/experiment="EXISTENCE:mutant phenotype:GO:0007032
endosome organization [PMID:23547030]"
/experiment="EXISTENCE:mutant phenotype:GO:0032880
regulation of protein localization [PMID:23547030]"
/note="Subunit of the BLOC-1 complex involved in endosomal
maturation; null mutant is sensitive to drug inducing
secretion of vacuolar cargo; GFP-fusion protein localizes
to the endosome"
/codon_start=1
/product="Kxd1p"
/protein_id="NP_011436.1"
/db_xref="GeneID:852801"
/db_xref="SGD:S000003047"
/translation="MVTGISEENDDEETFSAVHSSTPSINSQSYAIPITEEMSSSFHD
SISTTSNSSGSFDSDGSNVSDVVEQNEMDNESNVDEDLFLDNDIPQSSNLLPTDAQDP
GPIFDVSRYIFDSLKQSIDSADFSEALSLQTKTSAVINSKSLELKQYIDEMKSRLTQL
QEKFENGEATSKKIKRDLETSRKNIDYLNAALRVDFPIEFNQAREKILERRLNEDHDC
"
gene complement(<360288..>361859)
/gene="DBP3"
/locus_tag="YGL078C"
/db_xref="GeneID:852802"
mRNA complement(<360288..>361859)
/gene="DBP3"
/locus_tag="YGL078C"
/product="RNA-dependent ATPase DBP3"
/transcript_id="NM_001180943.3"
/db_xref="GeneID:852802"
CDS complement(360288..361859)
/gene="DBP3"
/locus_tag="YGL078C"
/EC_number="3.6.4.13"
/experiment="EXISTENCE:direct assay:GO:0003724 RNA
helicase activity [PMID:23153376]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:9032262]"
/experiment="EXISTENCE:direct assay:GO:0008186
ATP-dependent activity, acting on RNA
[PMID:18975973|PMID:23153376]"
/experiment="EXISTENCE:direct assay:GO:0030687
preribosome, large subunit precursor [PMID:22083961]"
/experiment="EXISTENCE:mutant phenotype:GO:0000464
endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA
from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA,
LSU-rRNA) [PMID:9032262]"
/note="RNA-Dependent ATPase, member of DExD/H-box family;
involved in cleavage of site A3 within the ITS1 spacer
during rRNA processing; not essential for growth, but
deletion causes severe slow-growth phenotype"
/codon_start=1
/product="RNA-dependent ATPase DBP3"
/protein_id="NP_011437.3"
/db_xref="GeneID:852802"
/db_xref="SGD:S000003046"
/translation="MTKEEIADKKRKVVDEEVIEKKKSKKHKKDKKDKKEKKDKKHKK
HKKEKKGEKEVEVPEKESEKKPEPTSAVASEFYVQSEALTSLPQSDIDEYFKENEIAV
EDSLDLALRPLLSFDYLSLDSSIQAEISKFPKPTPIQAVAWPYLLSGKDVVGVAETGS
GKTFAFGVPAISHLMNDQKKRGIQVLVISPTRELASQIYDNLIVLTDKVGMQCCCVYG
GVPKDEQRIQLKKSQVVVATPGRLLDLLQEGSVDLSQVNYLVLDEADRMLEKGFEEDI
KNIIRETDASKRQTLMFTATWPKEVRELASTFMNNPIKVSIGNTDQLTANKRITQIVE
VVDPRGKERKLLELLKKYHSGPKKNEKVLIFALYKKEAARVERNLKYNGYNVAAIHGD
LSQQQRTQALNEFKSGKSNLLLATDVAARGLDIPNVKTVINLTFPLTVEDYVHRIGRT
GRAGQTGTAHTLFTEQEKHLAGGLVNVLNGANQPVPEDLIKFGTHTKKKEHSAYGSFF
KDVDLTKKPKKITFD"
gene complement(<362225..>363916)
/gene="HNM1"
/locus_tag="YGL077C"
/gene_synonym="CTR1"
/db_xref="GeneID:852803"
mRNA complement(<362225..>363916)
/gene="HNM1"
/locus_tag="YGL077C"
/gene_synonym="CTR1"
/product="Hnm1p"
/transcript_id="NM_001180942.1"
/db_xref="GeneID:852803"
CDS complement(362225..363916)
/gene="HNM1"
/locus_tag="YGL077C"
/gene_synonym="CTR1"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:24187140]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0015220 choline
transmembrane transporter activity
[PMID:3514579|PMID:2203793]"
/experiment="EXISTENCE:mutant phenotype:GO:0015871 choline
transport [PMID:2203793]"
/experiment="EXISTENCE:mutant phenotype:GO:0031460 glycine
betaine transport [PMID:16487344]"
/experiment="EXISTENCE:mutant phenotype:GO:0034228
ethanolamine transmembrane transporter activity
[PMID:3514579]"
/experiment="EXISTENCE:mutant phenotype:GO:0034229
ethanolamine transport [PMID:3514579]"
/experiment="EXISTENCE:mutant phenotype:GO:1900749
(R)-carnitine transport [PMID:23755272]"
/experiment="EXISTENCE:mutant phenotype:GO:1901235
(R)-carnitine transmembrane transporter activity
[PMID:23755272]"
/note="Plasma membrane transporter for choline,
ethanolamine, and carnitine; involved in the uptake of
nitrogen mustard and the uptake of glycine betaine during
hypersaline stress; co-regulated with phospholipid
biosynthetic genes and negatively regulated by choline and
myo-inositol; human FLVCR2 (MFSD7c), a choline transporter
involved in Fowler syndrome, can complement the yeast null
mutant"
/codon_start=1
/product="Hnm1p"
/protein_id="NP_011438.1"
/db_xref="GeneID:852803"
/db_xref="SGD:S000003045"
/translation="MSIRNDNASGGYMQPDQSSNASMHKRDLRVEEEIKPLDDMDSKG
AVAADGEVHLRKSFSLWSILGVGFGLTNSWFGISTSMVAGISSGGPMMIVYGIIIVAL
ISICIGTSLGELSSAYPHAGGQFWWSLKLAPPKYKRFAAYMCGSFAYAGSVFTSASTT
LSVATEVVGMYALTHPEFIPKRWHIFVCFELLHLFLMFFNCYGKSLPIISSSSLYISL
LSFFTITITVLACSHGKFNDAKFVFATFNNETGWKNGGIAFIVGLINPAWSFSCLDCA
THMAFEVEKPERVIPIAIMGTVAIGFVTSFCYVIAMFFSIQDLDAVLSSTTGAPILDI
YNQALGNKSGAIFLGCLILFTSFGCVIACHTWQARLCWSFARDNGLPLSRLWSQVNPH
TGVPLNAHLMSCAWITLIGLLYLASSTAFQSLITGCIAFLLLSYIIPVICLLAKKRNI
AHGPFWLGKFGFFSNIVLLGWTVFSVVFFSFPPVLPVTKDNMNYVCVVIVGYTAYSIL
YWKYKGKKEFHALEESENEQAEYSNNFDTIEDSREFSVAASDVELENEHVPWGKK"
gene complement(<364335..>365996)
/gene="RPL7A"
/locus_tag="YGL076C"
/db_xref="GeneID:852804"
mRNA complement(join(<364335..364964,365433..365526,
365986..>365996))
/gene="RPL7A"
/locus_tag="YGL076C"
/product="60S ribosomal protein uL30 RPL7A"
/transcript_id="NM_001180941.1"
/db_xref="GeneID:852804"
CDS complement(join(364335..364964,365433..365526,
365986..365996))
/gene="RPL7A"
/locus_tag="YGL076C"
/experiment="EXISTENCE:direct assay:GO:0002181 cytoplasmic
translation [PMID:18782943]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:18782943]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:19466602]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:18782943]"
/experiment="EXISTENCE:direct assay:GO:0042273 ribosomal
large subunit biogenesis [PMID:23945946]"
/experiment="EXISTENCE:mutant phenotype:GO:0000470
maturation of LSU-rRNA [PMID:22893726]"
/note="Ribosomal 60S subunit protein L7A; required for
processing of 27SA3 pre-rRNA to 27SB pre-rRNA during
assembly of large ribosomal subunit; depletion leads to a
turnover of pre-rRNA; contains a conserved C-terminal
Nucleic acid Binding Domain (NDB2); binds to Domain II of
25S and 5.8S rRNAs; homologous to mammalian ribosomal
protein L7 and bacterial L30; RPL7A has a paralog, RPL7B,
that arose from the whole genome duplication"
/codon_start=1
/product="60S ribosomal protein uL30 RPL7A"
/protein_id="NP_011439.1"
/db_xref="GeneID:852804"
/db_xref="SGD:S000003044"
/translation="MAAEKILTPESQLKKSKAQQKTAEQVAAERAARKAANKEKRAII
LERNAAYQKEYETAERNIIQAKRDAKAAGSYYVEAQHKLVFVVRIKGINKIPPKPRKV
LQLLRLTRINSGTFVKVTKATLELLKLIEPYVAYGYPSYSTIRQLVYKRGFGKINKQR
VPLSDNAIIEANLGKYGILSIDDLIHEIITVGPHFKQANNFLWPFKLSNPSGGWGVPR
KFKHFIQGGSFGNREEFINKLVKSMN"
gene complement(365163..365251)
/gene="SNR39"
/locus_tag="YNCG0014C"
/db_xref="GeneID:9164895"
ncRNA complement(365163..365251)
/ncRNA_class="snoRNA"
/gene="SNR39"
/locus_tag="YNCG0014C"
/product="SNR39"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:8674114]"
/experiment="EXISTENCE:curator inference:GO:0031428 box
C/D methylation guide snoRNP complex [PMID:8674114]"
/experiment="EXISTENCE:physical interaction:GO:0031428 box
C/D methylation guide snoRNP complex [PMID:10733567]"
/note="C/D box small nucleolar RNA (snoRNA); guides
2'-O-methylation of large subunit (LSU) rRNA at position
A807"
/transcript_id="NR_132180.1"
/db_xref="GeneID:9164895"
/db_xref="SGD:S000007302"
gene complement(366374..366469)
/gene="SNR39B"
/locus_tag="YNCG0015C"
/db_xref="GeneID:9164896"
ncRNA complement(366374..366469)
/ncRNA_class="snoRNA"
/gene="SNR39B"
/locus_tag="YNCG0015C"
/product="SNR39B"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:10024243]"
/experiment="EXISTENCE:curator inference:GO:0031428 box
C/D methylation guide snoRNP complex [PMID:10024243]"
/experiment="EXISTENCE:mutant phenotype:GO:0030562 rRNA
2'-O-ribose methylation guide activity [PMID:10024243]"
/experiment="EXISTENCE:mutant phenotype:GO:0031167 rRNA
methylation [PMID:10024243]"
/note="C/D box small nucleolar RNA (snoRNA); guides
2'-O-methylation of large subunit (LSU) rRNA at position
G805"
/transcript_id="NR_132181.1"
/db_xref="GeneID:9164896"
/db_xref="SGD:S000007303"
gene complement(<366925..>368088)
/gene="MPS2"
/locus_tag="YGL075C"
/gene_synonym="MMC1"
/db_xref="GeneID:852805"
mRNA complement(<366925..>368088)
/gene="MPS2"
/locus_tag="YGL075C"
/gene_synonym="MMC1"
/product="Mps2p"
/transcript_id="NM_001180940.1"
/db_xref="GeneID:852805"
CDS complement(366925..368088)
/gene="MPS2"
/locus_tag="YGL075C"
/gene_synonym="MMC1"
/experiment="EXISTENCE:direct assay:GO:0000781 chromosome,
telomeric region [PMID:32967926]"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:10397772]"
/experiment="EXISTENCE:direct assay:GO:0005640 nuclear
outer membrane [PMID:32059771]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005816 spindle
pole body [PMID:10397772]"
/experiment="EXISTENCE:direct assay:GO:0034993 meiotic
nuclear membrane microtubule tethering complex
[PMID:32967926]"
/experiment="EXISTENCE:direct assay:GO:0044821 meiotic
telomere tethering at nuclear periphery [PMID:32967926]"
/experiment="EXISTENCE:direct assay:GO:0140444
cytoskeleton-nuclear membrane anchor activity
[PMID:32967926]"
/experiment="EXISTENCE:genetic interaction:GO:0030474
spindle pole body duplication [PMID:10654940]"
/experiment="EXISTENCE:mutant phenotype:GO:0000741
karyogamy [PMID:16923827]"
/experiment="EXISTENCE:mutant phenotype:GO:0030474 spindle
pole body duplication
[PMID:10654940|PMID:1869587|PMID:16923827]"
/experiment="EXISTENCE:mutant phenotype:GO:0034993 meiotic
nuclear membrane microtubule tethering complex
[PMID:32059771]"
/experiment="EXISTENCE:mutant phenotype:GO:0071988 protein
localization to spindle pole body [PMID:16923827]"
/experiment="EXISTENCE:mutant phenotype:GO:0140444
cytoskeleton-nuclear membrane anchor activity
[PMID:32059771]"
/note="Essential membrane protein localized at nuclear
envelope and SPBs; required for insertion of the newly
duplicated spindle pole body into the nuclear envelope;
potentially phosphorylated by Cdc28p; MPS2 has a paralog,
CSM4, that arose from the whole genome duplication"
/codon_start=1
/product="Mps2p"
/protein_id="NP_011440.1"
/db_xref="GeneID:852805"
/db_xref="SGD:S000003043"
/translation="MSNGAFDAIFEYAWGQIDKPISGDFIYGKDLPKLIEIIENIFQK
AQKSGSYELRLPLFSEINKDLFRTFSNTKTFFKIHKEEFDDIFFNLVNHPLREILENA
FIGVDSIPSDFIVSMNLNSPSKFLVENKNKNTEGAGISTPRKKLTESPIKLLSRNNIG
KALEVQVEELKRELTAKQSLLQENERQVSELKIRLETYQEKYASIQQRFSDLQKARQV
EDNQNSSRTSDPGSPLVTGIDQKAILEEFRRRLQRQTDTISFLKDQIRRERGLNCSND
KVSHSKRKHATTDGDGTFKNFISAVPSNIWVKATIRIIVCFALLAGVLPYIRKYVYAH
DTPSQNSRLQLSWWENSGILSKIVWFFEDQTDLETEYRSNANVDDAYSRVFGI"
gene <368753..>371254
/gene="HSF1"
/locus_tag="YGL073W"
/gene_synonym="EXA3; MAS3"
/db_xref="GeneID:852806"
mRNA <368753..>371254
/gene="HSF1"
/locus_tag="YGL073W"
/gene_synonym="EXA3; MAS3"
/product="stress-responsive transcription factor HSF1"
/transcript_id="NM_001180938.3"
/db_xref="GeneID:852806"
CDS 368753..371254
/gene="HSF1"
/locus_tag="YGL073W"
/gene_synonym="EXA3; MAS3"
/experiment="EXISTENCE:direct assay:GO:0003700 DNA-binding
transcription factor activity [PMID:3044612]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12821147]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0006357 regulation
of transcription by RNA polymerase II [PMID:3044612]"
/experiment="EXISTENCE:direct assay:GO:0043555 regulation
of translation in response to stress [PMID:31124783]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:19111667]"
/experiment="EXISTENCE:mutant phenotype:GO:0009408
response to heat [PMID:3044612|PMID:2017170|PMID:8985158]"
/experiment="EXISTENCE:mutant phenotype:GO:0034605
cellular response to heat [PMID:9296388]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:27320198|PMID:9296388]"
/experiment="EXISTENCE:mutant phenotype:GO:0070202
regulation of establishment of protein localization to
chromosome [PMID:23447536]"
/experiment="EXISTENCE:mutant phenotype:GO:1904262
negative regulation of TORC1 signaling [PMID:18270585]"
/note="Trimeric heat shock transcription factor; forms
condensate with Mediator and RNA Pol II upon heat shock;
activates multiple genes in response to diverse stresses
and transient intracellular acidification; recognizes
variable heat shock elements (HSEs) consisting of inverted
NGAAN repeats; monitors translational status through RQC
(Ribosomal Quality Control)-mediated translation-stress
signal; human homolog HSF1 with linker region mutations
can complement yeast hsf1 mutant"
/codon_start=1
/product="stress-responsive transcription factor HSF1"
/protein_id="NP_011442.3"
/db_xref="GeneID:852806"
/db_xref="SGD:S000003041"
/translation="MNNAANTGTTNESNVSDAPRIEPLPSLNDDDIEKILQPNDIFTT
DRTDASTTSSTAIEDIINPSLDPQSAASPVPSSSFFHDSRKPSTSTHLVRRGTPLGIY
QTNLYGHNSRENTNPNSTLLSSKLLAHPPVPYGQNPDLLQHAVYRAQPSSGTTNAQPR
QTTRRYQSHKSRPAFVNKLWSMLNDDSNTKLIQWAEDGKSFIVTNREEFVHQILPKYF
KHSNFASFVRQLNMYGWHKVQDVKSGSIQSSSDDKWQFENENFIRGREDLLEKIIRQK
GSSNNHNSPSGNGNPANGSNIPLDNAAGSNNSNNNISSSNSFFNNGHLLQGKTLRLMN
EANLGDKNDVTAILGELEQIKYNQIAISKDLLRINKDNELLWQENMMARERHRTQQQA
LEKMFRFLTSIVPHLDPKMIMDGLGDPKVNNEKLNSANNIGLNRDNTGTIDELKSNDS
FINDDRNSFTNATTNARNNMSPNNDDNSIDTASTNTTNRKKNIDENIKNNNDIINDII
FNTNLANNLSNYNSNNNAGSPIRPYKQRYLLKNRANSSTSSENPSLTPFDIESNNDRK
ISEIPFDDEEEEETDFRPFTSRDPNNQTSENTFDPNRFTMLSDDDLKKDSHTNDNKHN
ESDLFWDNVHRNIDEQDARLQNLENMVHILSPGYPNKSFNNKTSSTNTNSNMESAVNV
NSPGFNLQDYLTGESNSPNSVHSVPSNGSGSTPLPMPNDNDTEHASTSVNQGENGSGL
TPFLTVDDHTLNDNNTSEGSTRVSPDIKFSATENTKVSDNLPSFNDHSYSTQADTAPE
NAKKRFVEEIPEPAIVEIQDPTEYNDHRLPKRAKK"
gene <372012..>374084
/gene="AFT1"
/locus_tag="YGL071W"
/gene_synonym="RCS1"
/db_xref="GeneID:852809"
mRNA <372012..>374084
/gene="AFT1"
/locus_tag="YGL071W"
/gene_synonym="RCS1"
/product="DNA-binding transcription factor AFT1"
/transcript_id="NM_001180936.1"
/db_xref="GeneID:852809"
CDS 372012..374084
/gene="AFT1"
/locus_tag="YGL071W"
/gene_synonym="RCS1"
/experiment="EXISTENCE:direct assay:GO:0000981 DNA-binding
transcription factor activity, RNA polymerase II-specific
[PMID:8670839|PMID:27402848]"
/experiment="EXISTENCE:direct assay:GO:0000987
cis-regulatory region sequence-specific DNA binding
[PMID:8670839]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11877447|PMID:22842922|PMID:16911514]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:16911514|PMID:11877447|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0043515 kinetochore
binding [PMID:16172405]"
/experiment="EXISTENCE:direct assay:GO:0045944 positive
regulation of transcription by RNA polymerase II
[PMID:14739928]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:27402848]"
/experiment="EXISTENCE:mutant phenotype:GO:0007059
chromosome segregation [PMID:16172405]"
/experiment="EXISTENCE:mutant phenotype:GO:0010106
cellular response to iron ion starvation
[PMID:7720713|PMID:12095998]"
/experiment="EXISTENCE:mutant phenotype:GO:0032048
cardiolipin metabolic process [PMID:27402848]"
/experiment="EXISTENCE:mutant phenotype:GO:0034087
establishment of mitotic sister chromatid cohesion
[PMID:22157760]"
/experiment="EXISTENCE:mutant phenotype:GO:0034758
positive regulation of iron ion transport [PMID:7720713]"
/experiment="EXISTENCE:mutant phenotype:GO:0045132 meiotic
chromosome segregation [PMID:22157760]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:12095998|PMID:7720713]"
/note="Transcription factor involved in iron utilization
and homeostasis; binds consensus site PyPuCACCCPu and
activates transcription in response to changes in iron
availability; in iron-replete conditions localization is
regulated by Grx3p, Grx4p, and Fra2p, and promoter binding
is negatively regulated via Grx3p-Grx4p binding; AFT1 has
a paralog, AFT2, that arose from the whole genome
duplication; relative distribution to the nucleus
increases upon DNA replication stress"
/codon_start=1
/product="DNA-binding transcription factor AFT1"
/protein_id="NP_011444.1"
/db_xref="GeneID:852809"
/db_xref="SGD:S000003039"
/translation="MEGFNPADIEHASPINSSDSHSSSFVYALPKSASEYVVNHNEGR
ASASGNPAAVPSPIMTLNLKSTHSLNIDQHVHTSTSPTETIGHIHHVEKLNQNNLIHL
DPVPNFEDKSDIKPWLQKIFYPQGIELVIERSDAFKVVFKCKAAKRGRNARRKRKDKP
KGQDHEDEKSKINDDELEYASPSNATVTNGPQTSPDQTSSIKPKKKRCVSRFNNCPFR
VRATYSLKRKRWSIVVMDNNHSHQLKFNPDSEEYKKFKEKLRKDNDVDAIKKFDELEY
RTLANLPIPTATIPCDCGLTNEIQSFNVVLPTNSNVTSSASSSTVSSISLDSSNASKR
PCLPSVNNTGSINTNNVRKPKSQCKNKDTLLKRTTMQNFLTTKSRLRKTGTPTSSQHS
STAFSGYIDDPFNLNEILPLPASDFKLNTVTNLNEIDFTNIFTKSPHPHSGSTHPRQV
FDQLDDCSSILFSPLTTNTNNEFEGESDDFVHSPYLNSEADFSQILSSAPPVHHDPNE
THQENQDIIDRFANSSQEHNEYILQYLTHSDAANHNNIGVPNNNSHSLNTQHNVSDLG
NSLLRQEALVGSSSTKIFDELKFVQNGPHGSQHPIDFQHVDHRHLSSNEPQVRSHQYG
PQQQPPQQLQYHQNQPHDGHNHEQHQTVQKDMQTHESLEIMGNTLLEEFKDIKMVNGE
LKYVKPED"
gene complement(<374459..>374827)
/gene="RPB9"
/locus_tag="YGL070C"
/gene_synonym="SSU73"
/db_xref="GeneID:852810"
mRNA complement(<374459..>374827)
/gene="RPB9"
/locus_tag="YGL070C"
/gene_synonym="SSU73"
/product="DNA-directed RNA polymerase II core subunit
RPB9"
/transcript_id="NM_001180935.1"
/db_xref="GeneID:852810"
CDS complement(374459..374827)
/gene="RPB9"
/locus_tag="YGL070C"
/gene_synonym="SSU73"
/experiment="EXISTENCE:direct assay:GO:0003968
RNA-directed RNA polymerase activity [PMID:18004386]"
/experiment="EXISTENCE:direct assay:GO:0005665 RNA
polymerase II, core complex
[PMID:2186966|PMID:2183013|PMID:1331084]"
/experiment="EXISTENCE:genetic interaction:GO:0001193
maintenance of transcriptional fidelity during
transcription elongation by RNA polymerase II
[PMID:17535246]"
/experiment="EXISTENCE:genetic interaction:GO:0006283
transcription-coupled nucleotide-excision repair
[PMID:17030604]"
/experiment="EXISTENCE:mutant phenotype:GO:0001193
maintenance of transcriptional fidelity during
transcription elongation by RNA polymerase II
[PMID:16492753]"
/experiment="EXISTENCE:mutant phenotype:GO:0006366
transcription by RNA polymerase II [PMID:17179178]"
/experiment="EXISTENCE:mutant phenotype:GO:0006367
transcription initiation at RNA polymerase II promoter
[PMID:7800482]"
/note="RNA polymerase II subunit B12.6; contacts DNA;
mutations affect transcription start site selection and
fidelity of transcription; positively regulates ATG1
transcription as a key regulator of autophagy"
/codon_start=1
/product="DNA-directed RNA polymerase II core subunit
RPB9"
/protein_id="NP_011445.1"
/db_xref="GeneID:852810"
/db_xref="SGD:S000003038"
/translation="MTTFRFCRDCNNMLYPREDKENNRLLFECRTCSYVEEAGSPLVY
RHELITNIGETAGVVQDIGSDPTLPRSDRECPKCHSRENVFFQSQQRRKDTSMVLFFV
CLSCSHIFTSDQKNKRTQFS"
gene <375087..>375671
/gene="MNP1"
/locus_tag="YGL068W"
/gene_synonym="bL12m"
/db_xref="GeneID:852811"
mRNA <375087..>375671
/gene="MNP1"
/locus_tag="YGL068W"
/gene_synonym="bL12m"
/product="mitochondrial 54S ribosomal protein bL12m MNP1"
/transcript_id="NM_001180933.1"
/db_xref="GeneID:852811"
CDS 375087..375671
/gene="MNP1"
/locus_tag="YGL068W"
/gene_synonym="bL12m"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:24675956]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:24675956]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:24769239|PMID:16823961|PMID:14576278|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005762
mitochondrial large ribosomal subunit [PMID:24675956]"
/experiment="EXISTENCE:direct assay:GO:0042645
mitochondrial nucleoid [PMID:15221522]"
/note="Mitochondrial ribosomal protein of the large
subunit; has similarity to E. coli L7/L12 and human MRPL7
ribosomal proteins; associates with the mitochondrial
nucleoid; required for normal respiratory growth"
/codon_start=1
/product="mitochondrial 54S ribosomal protein bL12m MNP1"
/protein_id="NP_011447.1"
/db_xref="GeneID:852811"
/db_xref="SGD:S000003036"
/translation="MSLRILAKRSSSIWMKTRVTPALISPITITTRFNSTTTTAPSHK
DDVRPVDPKISKIVQDISQLTLLETSSLINELKTVLNIPEISMPMGGFMAGAAGAGAG
NVPSSTGEAGSGAEEEAKPEAKTVFTVKLDSFDTKTKAKVIKEVKGLLGLSLVEAKKF
VEAAPKVLKENVAKDDAEKIKKTLEDLGAKVSLE"
gene <376101..>377255
/gene="NPY1"
/locus_tag="YGL067W"
/db_xref="GeneID:852813"
mRNA <376101..>377255
/gene="NPY1"
/locus_tag="YGL067W"
/product="NAD(+) diphosphatase"
/transcript_id="NM_001180932.3"
/db_xref="GeneID:852813"
CDS 376101..377255
/gene="NPY1"
/locus_tag="YGL067W"
/EC_number="3.6.1.22"
/experiment="EXISTENCE:direct assay:GO:0000210 NAD+
diphosphatase activity [PMID:11361135]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:11361135]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0019674 NAD+
metabolic process [PMID:11361135]"
/experiment="EXISTENCE:mutant phenotype:GO:0110154 RNA
decapping [PMID:34074215]"
/experiment="EXISTENCE:mutant phenotype:GO:0110155 NAD-cap
decapping [PMID:33139726]"
/note="NADH diphosphatase (pyrophosphatase); hydrolyzes
the pyrophosphate linkage in NADH and related nucleotides;
localizes to peroxisomes; enzymatic component of one of
several partly redundant surveillance pathways involved in
decapping of non-canonical nicotinamide adenine
dinucleotide (NAD) capped nuclear mRNAs (NAD-RNAs); also
possesses deCoAping activity involved in the decapping of
non-canonical dephospho-CoA capped RNA (dpCoA-RNA); nudix
hydrolase family member"
/codon_start=1
/product="NAD(+) diphosphatase"
/protein_id="NP_011448.3"
/db_xref="GeneID:852813"
/db_xref="SGD:S000003035"
/translation="MSTAVTFFGQHVLNRVSFLRCSKEFIKKSLNHDSTVFIPFIEGE
ALISPENGDLVQLSNSVKSYKNILSAIVPLYTTLLNTTRSRSDESGINVTFLGLLEGT
DSAFNFEWSNISYKGTPYFGLDIRVTESTLFKKVDFEPIFSYPKVTRDHIFKQTNEDA
SLYSQGKMYLDWLAKYKFCPGCGSPLFPVEAGTKLQCSNENRNVYCNVRDARINNVCF
PRTDPTVIIALTNSDYSKCCLARSKKRYGDFVLYSTIAGFMEPSETIEEACIREIWEE
TGISCKNIDIVRSQPWPYPCSLMIGCLGIVQFNSKNEVINLNHDDELLDAQWFDTTEI
IQALDKYAGGYRVPFKNDINLPGSTTIAFQLINHVCENYKNLRKTSSSHL"
gene <377609..>379582
/gene="SGF73"
/locus_tag="YGL066W"
/gene_synonym="SCA7"
/db_xref="GeneID:852814"
mRNA <377609..>379582
/gene="SGF73"
/locus_tag="YGL066W"
/gene_synonym="SCA7"
/product="deubiquitination module subunit SGF73"
/transcript_id="NM_001180931.1"
/db_xref="GeneID:852814"
CDS 377609..379582
/gene="SGF73"
/locus_tag="YGL066W"
/gene_synonym="SCA7"
/experiment="EXISTENCE:direct assay:GO:0000124 SAGA
complex [PMID:12052880|PMID:17090597]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0008047 enzyme
activator activity [PMID:18488019]"
/experiment="EXISTENCE:direct assay:GO:0046822 regulation
of nucleocytoplasmic transport [PMID:31165730]"
/experiment="EXISTENCE:direct assay:GO:0071819 DUBm
complex [PMID:20395473]"
/experiment="EXISTENCE:direct assay:GO:1905634 regulation
of protein localization to chromatin [PMID:31165730]"
/experiment="EXISTENCE:genetic interaction:GO:0005198
structural molecule activity [PMID:15932941]"
/experiment="EXISTENCE:mutant phenotype:GO:0004402 histone
acetyltransferase activity [PMID:15932941]"
/experiment="EXISTENCE:mutant phenotype:GO:0005198
structural molecule activity [PMID:15932941]"
/experiment="EXISTENCE:mutant phenotype:GO:0006325
chromatin organization [PMID:15932941|PMID:17090597]"
/experiment="EXISTENCE:mutant phenotype:GO:0006406 mRNA
export from nucleus [PMID:18488019]"
/experiment="EXISTENCE:mutant phenotype:GO:0008047 enzyme
activator activity [PMID:20434206]"
/experiment="EXISTENCE:mutant phenotype:GO:0045899
positive regulation of RNA polymerase II transcription
preinitiation complex assembly [PMID:17090597]"
/experiment="EXISTENCE:mutant phenotype:GO:0065003
protein-containing complex assembly [PMID:19226466]"
/experiment="EXISTENCE:physical interaction:GO:0000124
SAGA complex [PMID:15932941]"
/experiment="EXISTENCE:physical interaction:GO:0046695
SLIK (SAGA-like) complex [PMID:15932941]"
/note="Subunit of DUBm module of SAGA and SLIK; has roles
in anchoring deubiquitination module (DUBm) into SAGA and
SLIK complexes, maintaining organization and
ubiquitin-binding conformation of Ubp8p, thereby
contributing to overall DUBm activity; involved in
preinitiation complex assembly at promoters; relocalizes
to cytosol under hypoxia; human homolog ATXN7 implicated
in spinocerebellar ataxia, and can complement yeast null
mutant"
/codon_start=1
/product="deubiquitination module subunit SGF73"
/protein_id="NP_011449.1"
/db_xref="GeneID:852814"
/db_xref="SGD:S000003034"
/translation="MRSGDAEIKGIKPKVIEEYSLSQGSGPSNDSWKSLMSSAKDTPL
QYDHMNRESLKKYFNPNAQLIEDPLDKPIQYRVCEKCGKPLALTAIVDHLENHCAGAS
GKSSTDPRDESTRETIRNGVESTGRNNNDDDNSNDNNNDDDDDDDNDDNEDDDDADDD
DDNSNGANYKKNDSSFNPLKRSTSMESANTPNMDTKRSKTGTPQTFSSSIKKQKKVKQ
RNPTEKHLIDFNKQCGVELPEGGYCARSLTCKSHSMGAKRAVSGRSKPYDVLLADYHR
EHQTKIGAAAEKRAKQQELQKLQKQIQKEQKKHTQQQKQGQRSKQRNVNGGKSAKNGG
KSTVHNGNNINEIGHVNLTPEEETTQVLNGVSRSFPLPLESTVLSSVRYRTKYFRMRE
MFASSFSVKPGYTSPGYGAIHSRVGCLDLDRTTDYKFRVRTPQPINHLTNQNLNPKQI
QRLQQQRALQAQLLSQQQQQQQQQQQHHSPQAQAQASTQQPTQGMVPNHFPGGATNSS
FNANVSSKQIQQQQQQQQHKSQDTGLTPLEIQSQQQKLRQQQLQQQKFEAAASYLANA
TKLMQESNQDSHLSGTHNNNSSKNGNNNLMTMKASISSPNTSVNSIQSPPSVNSVNGS
GQGVSTGINVSGNNGRIEVGIGNSVNPYNGRIN"
gene complement(<379760..>381271)
/gene="ALG2"
/locus_tag="YGL065C"
/db_xref="GeneID:852815"
mRNA complement(<379760..>381271)
/gene="ALG2"
/locus_tag="YGL065C"
/product="GDP-Man:Man(1)GlcNAc(2)-PP-dolichol
alpha-1,3-mannosyltransferase"
/transcript_id="NM_001180930.1"
/db_xref="GeneID:852815"
CDS complement(379760..381271)
/gene="ALG2"
/locus_tag="YGL065C"
/EC_number="2.4.1.132"
/EC_number="2.4.1.257"
/experiment="EXISTENCE:direct assay:GO:0004378
GDP-Man:Man(1)GlcNAc(2)-PP-Dol
alpha-1,3-mannosyltransferase activity [PMID:16878994]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0006488
dolichol-linked oligosaccharide biosynthetic process
[PMID:16878994]"
/experiment="EXISTENCE:direct assay:GO:0033164
initiation-specific glycolipid
1,6-alpha-mannosyltransferase activity [PMID:16878994]"
/note="Mannosyltransferase in the N-linked glycosylation
pathway; catalyzes two consecutive steps in N-linked
glycosylation pathway; mutants exhibit
temperature-sensitive growth and abnormal accumulation of
lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol; mutations
of human ALG2 cause congenital myasthenic syndrome; human
ALG2 complements temperature sensitivity and
dolichol-linked oligosaccharide biosynthesis defect of
alg2-1 mutant, but mutant form from a patient with CDG-Ii
fails to complement"
/codon_start=1
/product="GDP-Man:Man(1)GlcNAc(2)-PP-dolichol
alpha-1,3-mannosyltransferase"
/protein_id="NP_011450.1"
/db_xref="GeneID:852815"
/db_xref="SGD:S000003033"
/translation="MIEKDKRTIAFIHPDLGIGGAERLVVDAALGLQQQGHSVIIYTS
HCDKSHCFEEVKNGQLKVEVYGDFLPTNFLGRFFIVFATIRQLYLVIQLILQKKVNAY
QLIIIDQLSTCIPLLHIFSSATLMFYCHFPDQLLAQRAGLLKKIYRLPFDLIEQFSVS
AADTVVVNSNFTKNTFHQTFKYLSNDPDVIYPCVDLSTIEIEDIDKKFFKTVFNEGDR
FYLSINRFEKKKDVALAIKAFALSEDQINDNVKLVICGGYDERVAENVEYLKELQSLA
DEYELSHTTIYYQEIKRVSDLESFKTNNSKIIFLTSISSSLKELLLERTEMLLYTPAY
EHFGIVPLEAMKLGKPVLAVNNGGPLETIKSYVAGENESSATGWLKPAVPIQWATAID
ESRKILQNGSVNFERNGPLRVKKYFSREAMTQSFEENVEKVIWKEKKYYPWEIFGISF
SNFILHMAFIKILPNNPWPFLFMATFMVLYFKNYLWGIYWAFVFALSYPYEEI"
gene complement(<381523..>383208)
/gene="MRH4"
/locus_tag="YGL064C"
/db_xref="GeneID:852816"
mRNA complement(<381523..>383208)
/gene="MRH4"
/locus_tag="YGL064C"
/product="ATP-dependent RNA helicase"
/transcript_id="NM_001180929.1"
/db_xref="GeneID:852816"
CDS complement(381523..383208)
/gene="MRH4"
/locus_tag="YGL064C"
/EC_number="3.6.4.13"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:12702275|PMID:24769239|PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:24206665]"
/experiment="EXISTENCE:direct assay:GO:1990400
mitochondrial ribosomal large subunit rRNA binding
[PMID:24206665]"
/experiment="EXISTENCE:mutant phenotype:GO:0003724 RNA
helicase activity [PMID:24206665]"
/experiment="EXISTENCE:mutant phenotype:GO:0016070 RNA
metabolic process [PMID:12702275]"
/experiment="EXISTENCE:mutant phenotype:GO:1902775
mitochondrial large ribosomal subunit assembly
[PMID:24206665|PMID:31053642]"
/note="Mitochondrial ATP-dependent RNA helicase of the
DEAD-box family; required for assembly of the large
subunit of mitochondrial ribosomes; binds to the large
subunit rRNA, 21S_rRNA; localizes to the matrix face of
the mitochondrial inner membrane and associates with the
large subunit precursor and with mature ribosomes"
/codon_start=1
/product="ATP-dependent RNA helicase"
/protein_id="NP_011451.1"
/db_xref="GeneID:852816"
/db_xref="SGD:S000003032"
/translation="MSLFFKPVISPQWSFPVLLKIGVRSYAGGPRTKHKGNSPLASVP
TGSSNKNRKQKAKGKKGNKKNDPDQAFNFGEYGGLKKDVEMNMDSTNKLIQKISNFDQ
LLILPPVRDAVKEIISKESLKLQDSRKKTSENIIPSPIQTVAIKRISKNLMDPKLQIH
AIAAETGSGKTMAYLIPLIDYLKRQELETPELWETLRKNVLIRSIILVPTHELVDQVY
ETVSKTKTLLGLNSFKWDKATSYRDLLENIKNRIDILVTTPGKLLNLFSIRMITRPDK
VLSKVGFVVLDEADTLLDRSWLEETHSAIKRIPNINHLIFCSATIPQEFNKTMQRLFP
TVVPIMTPRLHKLPFALDFKVINSALSPFKGSKIKALAQTLYAISNDDTEPGFEKRCI
IFVNEKKNVPEIVNLLNKKFGHNAIGLTGEDTFEERSEKIMPFLSPPRPLSEVVAQST
SPPTSLKKFEIPDSNIVIGKLKNTNSNGTAPSNKSLHVLVTTDLMARGLNFKGVRNVV
LYDVPKTSIDLIHRVGRTARMKQGGRVFMLTDSKTKSWAKALPKIIKKHQRLS"
gene <383654..>384766
/gene="PUS2"
/locus_tag="YGL063W"
/db_xref="GeneID:852817"
mRNA <383654..>384766
/gene="PUS2"
/locus_tag="YGL063W"
/product="pseudouridine synthase PUS2"
/transcript_id="NM_001180928.4"
/db_xref="GeneID:852817"
CDS 383654..384766
/gene="PUS2"
/locus_tag="YGL063W"
/EC_number="5.4.99.44"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:17684231]"
/experiment="EXISTENCE:genetic interaction:GO:0009982
pseudouridine synthase activity [PMID:17684231]"
/experiment="EXISTENCE:genetic interaction:GO:0031119 tRNA
pseudouridine synthesis [PMID:17684231]"
/experiment="EXISTENCE:mutant phenotype:GO:0009982
pseudouridine synthase activity [PMID:17684231]"
/experiment="EXISTENCE:mutant phenotype:GO:0031119 tRNA
pseudouridine synthesis [PMID:17684231]"
/experiment="EXISTENCE:mutant phenotype:GO:1990481 mRNA
pseudouridine synthesis [PMID:25192136]"
/note="Mitochondrial tRNA:pseudouridine synthase; acts at
positions 27 and 28, but not at position 72; efficiently
and rapidly targeted to mitochondria, specifically
dedicated to mitochondrial tRNA modification; mutation
also affects pseudouridylation of some nuclear-encoded
mRNAs; PUS2 has a paralog, PUS1, that arose from the whole
genome duplication"
/codon_start=1
/product="pseudouridine synthase PUS2"
/protein_id="NP_011452.4"
/db_xref="GeneID:852817"
/db_xref="SGD:S000003031"
/translation="MLLGYCGSGYYGMQYNPPHKTIEGEILTKLFDVGAISEENSLAP
KKNSFMAAARTDKGVHAMLNLLSLKITLREDTVAKLNAALPPEIRVWGIQPVNKKFNA
RSACDSRWYQYLIPEFILIGPPRSSLLHRNVGGCYREDGSQEVWDTFLEQTRGRFSGD
ELCRLQDTAQKLSESDPLVQDYVGLLSGTLSGYCLSPSKLDAFEAAMQEYVGTHNFHN
FTTGKLWGDPSAQRHIKKVVVSQASPGWICVRIHGQSFMLHQIRRMVALAVLAARCQL
PPNIVRNYFNAGPRKYIPRAPAQGLLLEGPVFDGYNTKLRNLLYCEIRPDDITLERMC
RFRERQICTAIAHEETQRHVFCHFVRQMNRLATPLI"
gene <385196..>388732
/gene="PYC1"
/locus_tag="YGL062W"
/db_xref="GeneID:852818"
mRNA <385196..>388732
/gene="PYC1"
/locus_tag="YGL062W"
/product="pyruvate carboxylase 1"
/transcript_id="NM_001180927.1"
/db_xref="GeneID:852818"
CDS 385196..388732
/gene="PYC1"
/locus_tag="YGL062W"
/EC_number="6.4.1.1"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:196563|PMID:2665820]"
/experiment="EXISTENCE:mutant phenotype:GO:0004736
pyruvate carboxylase activity [PMID:2039506]"
/experiment="EXISTENCE:mutant phenotype:GO:0006094
gluconeogenesis [PMID:8185321]"
/note="Pyruvate carboxylase isoform; cytoplasmic enzyme
that converts pyruvate to oxaloacetate; differentially
regulated than isoform Pyc2p; mutations in the human
homolog are associated with lactic acidosis; PYC1 has a
paralog, PYC2, that arose from the whole genome
duplication"
/codon_start=1
/product="pyruvate carboxylase 1"
/protein_id="NP_011453.1"
/db_xref="GeneID:852818"
/db_xref="SGD:S000003030"
/translation="MSQRKFAGLRDNFNLLGEKNKILVANRGEIPIRIFRTAHELSMQ
TVAIYSHEDRLSTHKQKADEAYVIGEVGQYTPVGAYLAIDEIISIAQKHQVDFIHPGY
GFLSENSEFADKVVKAGITWIGPPAEVIDSVGDKVSARNLAAKANVPTVPGTPGPIET
VEEALDFVNEYGYPVIIKAAFGGGGRGMRVVREGDDVADAFQRATSEARTAFGNGTCF
VERFLDKPKHIEVQLLADNHGNVVHLFERDCSVQRRHQKVVEVAPAKTLPREVRDAIL
TDAVKLAKECGYRNAGTAEFLVDNQNRHYFIEINPRIQVEHTITEEITGIDIVAAQIQ
IAAGASLPQLGLFQDKITTRGFAIQCRITTEDPAKNFQPDTGRIEVYRSAGGNGVRLD
GGNAYAGTIISPHYDSMLVKCSCSGSTYEIVRRKMIRALIEFRIRGVKTNIPFLLTLL
TNPVFIEGTYWTTFIDDTPQLFQMVSSQNRAQKLLHYLADVAVNGSSIKGQIGLPKLK
SNPSVPHLHDAQGNVINVTKSAPPSGWRQVLLEKGPAEFARQVRQFNGTLLMDTTWRD
AHQSLLATRVRTHDLATIAPTTAHALAGRFALECWGGATFDVAMRFLHEDPWERLRKL
RSLVPNIPFQMLLRGANGVAYSSLPDNAIDHFVKQAKDNGVDIFRVFDALNDLEQLKV
GVDAVKKAGGVVEATVCFSGDMLQPGKKYNLDYYLEIAEKIVQMGTHILGIKDMAGTM
KPAAAKLLIGSLRAKYPDLPIHVHTHDSAGTAVASMTACALAGADVVDVAINSMSGLT
SQPSINALLASLEGNIDTGINVEHVRELDAYWAEMRLLYSCFEADLKGPDPEVYQHEI
PGGQLTNLLFQAQQLGLGEQWAETKRAYREANYLLGDIVKVTPTSKVVGDLAQFMVSN
KLTSDDVRRLANSLDFPDSVMDFFEGLIGQPYGGFPEPFRSDVLRNKRRKLTCRPGLE
LEPFDLEKIREDLQNRFGDVDECDVASYNMYPRVYEDFQKMRETYGDLSVLPTRSFLS
PLETDEEIEVVIEQGKTLIIKLQAVGDLNKKTGEREVYFDLNGEMRKIRVADRSQKVE
TVTKSKADMHDPLHIGAPMAGVIVEVKVHKGSLIKKGQPVAVLSAMKMEMIISSPSDG
QVKEVFVSDGENVDSSDLLVLLEDQVPVETKA"
rep_origin 388658..388891
/note="ARS717; Autonomously Replicating Sequence"
/db_xref="SGD:S000118457"
gene complement(<388968..>389711)
/gene="DUO1"
/locus_tag="YGL061C"
/db_xref="GeneID:852819"
mRNA complement(<388968..>389711)
/gene="DUO1"
/locus_tag="YGL061C"
/product="Duo1p"
/transcript_id="NM_001180926.1"
/db_xref="GeneID:852819"
CDS complement(388968..389711)
/gene="DUO1"
/locus_tag="YGL061C"
/experiment="EXISTENCE:direct assay:GO:0008017 microtubule
binding [PMID:16777964|PMID:15664196]"
/experiment="EXISTENCE:direct assay:GO:0008608 attachment
of spindle microtubules to kinetochore [PMID:17620411]"
/experiment="EXISTENCE:direct assay:GO:0031116 positive
regulation of microtubule polymerization [PMID:15664196]"
/experiment="EXISTENCE:direct assay:GO:0042729 DASH
complex [PMID:15640796|PMID:15664196]"
/experiment="EXISTENCE:direct assay:GO:0051010 microtubule
plus-end binding [PMID:20479465|PMID:17620411]"
/experiment="EXISTENCE:direct assay:GO:0051987 positive
regulation of attachment of spindle microtubules to
kinetochore [PMID:20479465|PMID:20479468]"
/note="Essential subunit of the Dam1 complex (aka DASH
complex); cooperates with Dam1p and Pse1p to connect the
DASH complex with microtubules (MT); couples kinetochores
to the force produced by MT depolymerization thereby
aiding in chromosome segregation; is transferred to the
kinetochore prior to mitosis"
/codon_start=1
/product="Duo1p"
/protein_id="NP_011454.1"
/db_xref="GeneID:852819"
/db_xref="SGD:S000003029"
/translation="MSEQSQLDDSTIDKLIPQIFNEMRSNLNNTTNKFPKSTGGGASD
NISANSNSIRSFNSITTQSLLKESESLDKITAMIKNVTAALKNNLPVYVNQVHEVCKS
TNSILDSWINIHSQAGYIHKLMSDQTYLKLINDRLHNENVNTNDEDGSTLHNVIALKK
KEILDLRQKLENRKGEKDAAPAKPPNQGLNPRYGVQSGRRPVPSAGISNNGRVRKTHV
PASKRPSGIPRVTNRWTKPTASSSRKMFR"
gene <390065..>391990
/gene="YBP2"
/locus_tag="YGL060W"
/gene_synonym="YBH1"
/db_xref="GeneID:852820"
mRNA <390065..>391990
/gene="YBP2"
/locus_tag="YGL060W"
/gene_synonym="YBH1"
/product="Ybp2p"
/transcript_id="NM_001180925.1"
/db_xref="GeneID:852820"
CDS 390065..391990
/gene="YBP2"
/locus_tag="YGL060W"
/gene_synonym="YBH1"
/experiment="EXISTENCE:direct assay:GO:0000776 kinetochore
[PMID:18286174]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0007052
mitotic spindle organization [PMID:18286174]"
/note="Central kinetochore associated protein; mediates
mitotic progression; interacts with several central
kinetochore proteins and centromeric histone Cse4p; role
in resistance to oxidative stress; similar to Slk19p; YBP2
has a paralog, YBP1, that arose from the whole genome
duplication"
/codon_start=1
/product="Ybp2p"
/protein_id="NP_011455.1"
/db_xref="GeneID:852820"
/db_xref="SGD:S000003028"
/translation="MYNEQVNSGKSIKEKERYLDALLKILKDNPVTLKEIGWDLPKGL
LQFFSRKNINVNIHLVFSPLVSSVMECFNELAINGNPKECLLTACELVSTLHIVLTET
GDSDEENEDLNDSNRNDASNITDELSVITPEIGHYMAKNTVEFIPNLKIYVLFEFMSL
LLKRVDTLYPSKFLAMVTSAIIKYVTTNVQAMDDPHFILRIVYNFCTNYSPAQPSASL
TDGISTNDLEKIHDDESALQKKLLANLSVFVISNCLKNHPGNIDKIYFKTLMHKKTDE
NEIDASVLQICHQYYEYVTSLDVHMKELLEKCLVESRSIYNSLLMNPAASTPEFKEEI
NQLVYEVSYAYQIKKLADEKNLELDQYGVVILSAIHYSKNGTHLLPQIDIQSAIYLYL
RCTTASLFSEIYENKFLESSVRYWLWVSTTETSTEKIKCALQELPGHITTAFLQMLLM
KTCNESNNDTKLTEITLLRRLLYLMPESTSFTFIFETLLHCPYITAKIAVLDILRDMM
IRSPEAANRDETVGLIEQQNPGNTANSVPIMPTLPPRPYITINEDRMASIHSIALICF
SAAKQKKRTQGDLLLVLTYMKFFVSLRNKWDLGLLTLINKEISESFQGEGEPELAFIN
ISNNTLGEYIEEMNIRS"
gene <392223..>393698
/gene="PKP2"
/locus_tag="YGL059W"
/db_xref="GeneID:852821"
mRNA <392223..>393698
/gene="PKP2"
/locus_tag="YGL059W"
/product="protein kinase PKP2"
/transcript_id="NM_001180924.4"
/db_xref="GeneID:852821"
CDS 392223..393698
/gene="PKP2"
/locus_tag="YGL059W"
/EC_number="2.7.11.2"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894|PMID:17918780]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:17918780|PMID:18180296|PMID:14562095|PMID:22842922|P
MID:16823961]"
/experiment="EXISTENCE:mutant phenotype:GO:0004740
pyruvate dehydrogenase (acetyl-transferring) kinase
activity [PMID:18180296]"
/experiment="EXISTENCE:mutant phenotype:GO:1901524
regulation of mitophagy [PMID:31548421]"
/note="Mitochondrial protein kinase; negatively regulates
activity of the pyruvate dehydrogenase complex by
phosphorylating the ser-133 residue of the Pda1p subunit;
acts in concert with kinase Pkp1p and phosphatases Ptc5p
and Ptc6p; relocalizes from mitochondrion to cytoplasm
upon DNA replication stress"
/codon_start=1
/product="protein kinase PKP2"
/protein_id="NP_011456.5"
/db_xref="GeneID:852821"
/db_xref="SGD:S000003027"
/translation="MSKYQINCIRYRHFLRTSNISQIPDFTKYCIGPVNEELAPYIME
TMKAYPSNSEYINPQHYYHNRTVLVENYLKRSPNPVSLTQLAQYYDDSTKLTRTKIIN
SGKFVKEELVIRIAHKLNQLQQLPFNVVNNFHFVQVYESYYNIFESFRKYPTIRTLED
ASQFADFIKNMLEGFNTLNLPHLIMGALECTILDLYPREKMDQLLSDLLRARISRRLI
VEEHVSITANYTSGKEENTLVLGDIFQECSAKKYLLEASEESQKFIQDMYFKDIPMPE
FIIEGDTQLSFYFLPTHLKYLLGEILRNTYEATMKHYIRKGLEKPEPIIVTVVSNDES
YLFRISDKAGGVLHDDENLWSFGKSKERAQESLNNFHKLPGLQTVSIYDEVHSHTKYN
SKLKSLQSITLKPYMHTSLEPMSYPSIINGHIKYETPLIELLKRSFRYKLGIGLAMCK
VYAEYWNGDLSLHSMPGYGTDVVLKLGNLMKHTKKLQLDKV"
gene <393986..>394504
/gene="RAD6"
/locus_tag="YGL058W"
/gene_synonym="PSO8; UBC2"
/db_xref="GeneID:852822"
mRNA <393986..>394504
/gene="RAD6"
/locus_tag="YGL058W"
/gene_synonym="PSO8; UBC2"
/product="E2 ubiquitin-conjugating protein RAD6"
/transcript_id="NM_001180923.1"
/db_xref="GeneID:852822"
CDS 393986..394504
/gene="RAD6"
/locus_tag="YGL058W"
/gene_synonym="PSO8; UBC2"
/EC_number="2.3.2.23"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:14752010]"
/experiment="EXISTENCE:direct assay:GO:0003697
single-stranded DNA binding [PMID:9287349]"
/experiment="EXISTENCE:direct assay:GO:0004842
ubiquitin-protein transferase activity
[PMID:3306404|PMID:9287349]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:8436296|PMID:10880451]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:10880451]"
/experiment="EXISTENCE:direct assay:GO:0017116
single-stranded DNA helicase activity [PMID:9287349]"
/experiment="EXISTENCE:direct assay:GO:0070628 proteasome
binding [PMID:10848595]"
/experiment="EXISTENCE:direct assay:GO:0097505 Rad6-Rad18
complex [PMID:9287349]"
/experiment="EXISTENCE:genetic interaction:GO:0000722
telomere maintenance via recombination [PMID:23390378]"
/experiment="EXISTENCE:genetic interaction:GO:0000724
double-strand break repair via homologous recombination
[PMID:16783014]"
/experiment="EXISTENCE:genetic interaction:GO:0036503 ERAD
pathway [PMID:23988329]"
/experiment="EXISTENCE:genetic interaction:GO:0042275
error-free postreplication DNA repair [PMID:9576943]"
/experiment="EXISTENCE:genetic interaction:GO:0042276
error-prone translesion synthesis [PMID:770231]"
/experiment="EXISTENCE:genetic interaction:GO:0070987
error-free translesion synthesis [PMID:9409821]"
/experiment="EXISTENCE:mutant phenotype:GO:0006353
DNA-templated transcription termination [PMID:23109428]"
/experiment="EXISTENCE:mutant phenotype:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:15504724|PMID:8436296]"
/experiment="EXISTENCE:mutant phenotype:GO:0009302
sno(s)RNA transcription [PMID:23109428]"
/experiment="EXISTENCE:mutant phenotype:GO:0031509
subtelomeric heterochromatin formation
[PMID:9343433|PMID:12535539]"
/experiment="EXISTENCE:mutant phenotype:GO:0031571 mitotic
G1 DNA damage checkpoint signaling
[PMID:15632126|PMID:16166626]"
/experiment="EXISTENCE:mutant phenotype:GO:0034620
cellular response to unfolded protein [PMID:20462952]"
/experiment="EXISTENCE:mutant phenotype:GO:0042138 meiotic
DNA double-strand break formation [PMID:15280549]"
/experiment="EXISTENCE:mutant phenotype:GO:0071596
ubiquitin-dependent protein catabolic process via the
N-end rule pathway [PMID:1651502|PMID:8505328]"
/experiment="EXISTENCE:mutant phenotype:GO:0071629
cytoplasm protein quality control by the
ubiquitin-proteasome system [PMID:20462952]"
/experiment="EXISTENCE:mutant phenotype:GO:0090089
regulation of dipeptide transport [PMID:9427760]"
/experiment="EXISTENCE:mutant phenotype:GO:0120174
stress-induced homeostatically regulated protein
degradation pathway [PMID:29861160]"
/experiment="EXISTENCE:mutant phenotype:GO:1990303
UBR1-RAD6 ubiquitin ligase complex [PMID:8505328]"
/experiment="EXISTENCE:physical interaction:GO:0006366
transcription by RNA polymerase II [PMID:15632065]"
/experiment="EXISTENCE:physical interaction:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:15504724]"
/experiment="EXISTENCE:physical interaction:GO:1990303
UBR1-RAD6 ubiquitin ligase complex
[PMID:1651502|PMID:8505328]"
/experiment="EXISTENCE:physical interaction:GO:1990304
MUB1-RAD6-UBR2 ubiquitin ligase complex [PMID:18070918]"
/note="Ubiquitin-conjugating enzyme (E2); involved in
postreplication repair as a heterodimer with Rad18p,
regulation of K63 polyubiquitination in response to
oxidative stress, DSBR and checkpoint control as a
heterodimer with Bre1p, ubiquitin-mediated N-end rule
protein degradation as a heterodimer with Ubr1p, ERAD with
Ubr1p in the absence of canonical ER membrane ligases, and
Rpn4p turnover as part of proteasome homeostasis, in
complex with Ubr2p and Mub1p"
/codon_start=1
/product="E2 ubiquitin-conjugating protein RAD6"
/protein_id="NP_011457.1"
/db_xref="GeneID:852822"
/db_xref="SGD:S000003026"
/translation="MSTPARRRLMRDFKRMKEDAPPGVSASPLPDNVMVWNAMIIGPA
DTPYEDGTFRLLLEFDEEYPNKPPHVKFLSEMFHPNVYANGEICLDILQNRWTPTYDV
ASILTSIQSLFNDPNPASPANVEAATLFKDHKSQYVKRVKETVEKSWEDDMDDMDDDD
DDDDDDDDDEAD"
gene complement(<394966..>395829)
/gene="GEP7"
/locus_tag="YGL057C"
/db_xref="GeneID:852823"
mRNA complement(<394966..>395829)
/gene="GEP7"
/locus_tag="YGL057C"
/product="Gep7p"
/transcript_id="NM_001180922.1"
/db_xref="GeneID:852823"
CDS complement(394966..395829)
/gene="GEP7"
/locus_tag="YGL057C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14562095]"
/note="hypothetical protein; null mutant exhibits a
respiratory growth defect and synthetic interactions with
prohibitin (phb1) and gem1; authentic, non-tagged protein
is detected in highly purified mitochondria in
high-throughput studies"
/codon_start=1
/product="Gep7p"
/protein_id="NP_011458.1"
/db_xref="GeneID:852823"
/db_xref="SGD:S000003025"
/translation="MVLSNVKIFRLKSHRAFRIGPMIKAVAGNLLVKRFYQPKLERIP
PASLLLKQKIRLAQNGSTTSTENPISFSQTMSEIFSVLQPSAPDLDEDETSGLKRDHL
LTERLNNGELGVIMNKFFNPSSTHNNQLIDTNILLQNFPKLSGNDLDLLDFAINEKMR
GNWNDLKQDFIQLWYYKSFGFLGPRTQFVLTNSSPSVRSQFLKLPFIEYNWFLLQNNK
NANILPADVQNVVKVFHLDDKRFTWKSIDPFSKAIISFVVFVSIYVWLDESAKQKTKE
LPAQKSTVISE"
gene complement(<396035..>397618)
/gene="SDS23"
/locus_tag="YGL056C"
/db_xref="GeneID:852824"
mRNA complement(<396035..>397618)
/gene="SDS23"
/locus_tag="YGL056C"
/product="Sds23p"
/transcript_id="NM_001180921.2"
/db_xref="GeneID:852824"
CDS complement(396035..397618)
/gene="SDS23"
/locus_tag="YGL056C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095|PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:11914276]"
/experiment="EXISTENCE:genetic interaction:GO:0000920
septum digestion after cytokinesis [PMID:16041152]"
/note="Protein involved in cell separation during budding;
one of two S. cerevisiae homologs (Sds23p and Sds24p) of
the S. pombe Sds23 protein, which is implicated in
APC/cyclosome regulation; SDS23 has a paralog, SDS24, that
arose from the whole genome duplication"
/codon_start=1
/product="Sds23p"
/protein_id="NP_011459.2"
/db_xref="GeneID:852824"
/db_xref="SGD:S000003024"
/translation="MPQNTRHTSIVEMLSTPPQLPNSTDLNSLSEQTDKNTEANKSDT
ESLHKSISKSSSSSSLSTLDNTEYSNNNGNSLSTLNSQNLLSVHRQEWQHTPLSNLVE
QNKLIFIRGSISVEEAFNTLVKHQLTSLPVENFPGDMNCLTFDYNDLNAYLLLVLNRI
KVSNDKITSDCQNGKSVPVGEIVKLTPKNPFYKLPETENLSTVIGILGSGVHRVAITN
VEMTQIKGILSQRRLIKYLWENARSFPNLKPLLDSSLEELNIGVLNAARDKPTFKQSR
VISIQGDEHLIMALHKMYVERISSIAVVDPQGNLIGNISVTDVKHVTRTSQYPLLHNT
CRHFVSVILNLRGLETGKDSFPIFHVYPTSSLARTFAKLVATKSHRLWIVQPNDNQPT
ASSEKSSSPSPSTPPVTTLPSLASSYHSNTQSSRMANSPVLKSSDTSNNKINVNINLS
GPSPSQPQSPSATMPPPQSPSNCPASPTPAHFEKEYRTGKLIGVVSLTDILSVLARKQ
THHKEIDPQMARKQRGHIG"
gene <398628..>400160
/gene="OLE1"
/locus_tag="YGL055W"
/gene_synonym="MDM2"
/db_xref="GeneID:852825"
mRNA <398628..>400160
/gene="OLE1"
/locus_tag="YGL055W"
/gene_synonym="MDM2"
/product="stearoyl-CoA 9-desaturase"
/transcript_id="NM_001180920.3"
/db_xref="GeneID:852825"
CDS 398628..400160
/gene="OLE1"
/locus_tag="YGL055W"
/gene_synonym="MDM2"
/EC_number="1.14.19.1"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:12475963]"
/experiment="EXISTENCE:genetic interaction:GO:0009055
electron transfer activity [PMID:8530368]"
/experiment="EXISTENCE:mutant phenotype:GO:0000001
mitochondrion inheritance [PMID:2202739]"
/experiment="EXISTENCE:mutant phenotype:GO:0004768
stearoyl-CoA 9-desaturase activity [PMID:1978720]"
/experiment="EXISTENCE:mutant phenotype:GO:0006636
unsaturated fatty acid biosynthetic process
[PMID:1978720]"
/note="Delta(9) fatty acid desaturase; required for
monounsaturated fatty acid synthesis and for normal
distribution of mitochondria"
/codon_start=1
/product="stearoyl-CoA 9-desaturase"
/protein_id="NP_011460.3"
/db_xref="GeneID:852825"
/db_xref="SGD:S000003023"
/translation="MPTSGTTIELIDDQFPKDDSASSGIVDEVDLTEANILATGLNKK
APRIVNGFGSLMGSKEMVSVEFDKKGNEKKSNLDRLLEKDNQEKEEAKTKIHISEQPW
TLNNWHQHLNWLNMVLVCGMPMIGWYFALSGKVPLHLNVFLFSVFYYAVGGVSITAGY
HRLWSHRSYSAHWPLRLFYAIFGCASVEGSAKWWGHSHRIHHRYTDTLRDPYDARRGL
WYSHMGWMLLKPNPKYKARADITDMTDDWTIRFQHRHYILLMLLTAFVIPTLICGYFF
NDYMGGLIYAGFIRVFVIQQATFCINSLAHYIGTQPFDDRRTPRDNWITAIVTFGEGY
HNFHHEFPTDYRNAIKWYQYDPTKVIIYLTSLVGLAYDLKKFSQNAIEEALIQQEQKK
INKKKAKINWGPVLTDLPMWDKQTFLAKSKENKGLVIISGIVHDVSGYISEHPGGETL
IKTALGKDATKAFSGGVYRHSNAAQNVLADMRVAVIKESKNSAIRMASKRGEIYETGK
FF"
gene complement(<400871..>401287)
/gene="ERV14"
/locus_tag="YGL054C"
/db_xref="GeneID:852826"
mRNA complement(<400871..>401287)
/gene="ERV14"
/locus_tag="YGL054C"
/product="cornichon family protein"
/transcript_id="NM_001180919.1"
/db_xref="GeneID:852826"
CDS complement(400871..401287)
/gene="ERV14"
/locus_tag="YGL054C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:9732282]"
/experiment="EXISTENCE:direct assay:GO:0030134
COPII-coated ER to Golgi transport vesicle
[PMID:11157978]"
/experiment="EXISTENCE:direct assay:GO:0038024 cargo
receptor activity [PMID:23270647]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:9732282]"
/experiment="EXISTENCE:mutant phenotype:GO:0007120 axial
cellular bud site selection [PMID:9732282]"
/experiment="EXISTENCE:mutant phenotype:GO:0030437
ascospore formation [PMID:9732282]"
/experiment="EXISTENCE:mutant phenotype:GO:0038024 cargo
receptor activity [PMID:23270647]"
/note="COPII-coated vesicle protein; involved in vesicle
formation and incorporation of specific secretory cargo;
required for the delivery of bud-site selection protein
Axl2p and Nha1p antiporter to cell surface; related to
Drosophila cornichon; ERV14 has a paralog, ERV15, that
arose from the whole genome duplication"
/codon_start=1
/product="cornichon family protein"
/protein_id="NP_011461.1"
/db_xref="GeneID:852826"
/db_xref="SGD:S000003022"
/translation="MGAWLFILAVVVNCINLFGQVHFTILYADLEADYINPIELCSKV
NKLITPEAALHGALSLLFLLNGYWFVFLLNLPVLAYNLNKIYNKVQLLDATEIFRTLG
KHKRESFLKLGFHLLMFFFYLYRMIMALIAESGDDF"
gene complement(401527..401598)
/locus_tag="YNCG0016C"
/db_xref="GeneID:852827"
tRNA complement(401527..401598)
/locus_tag="YNCG0016C"
/product="tRNA-Glu"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE
analysis; thiolation of uridine at wobble position (34)
requires Ncs6p"
/db_xref="GeneID:852827"
/db_xref="SGD:S000006554"
repeat_region 401616..401894
/note="Ty3 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006949"
repeat_region 401959..402290
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006944"
gene <402589..>403302
/gene="PRM8"
/locus_tag="YGL053W"
/db_xref="GeneID:852828"
mRNA <402589..>403302
/gene="PRM8"
/locus_tag="YGL053W"
/product="pheromone-regulated DUP240 family protein PRM8"
/transcript_id="NM_001180918.1"
/db_xref="GeneID:852828"
CDS 402589..403302
/gene="PRM8"
/locus_tag="YGL053W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:12925749]"
/experiment="EXISTENCE:mutant phenotype:GO:0005783
endoplasmic reticulum [PMID:12101299]"
/experiment="EXISTENCE:mutant phenotype:GO:0005886 plasma
membrane [PMID:12101299]"
/note="Pheromone-regulated protein; contains with 2
predicted transmembrane segments and an FF sequence, a
motif involved in COPII binding; forms a complex with
Prp9p in the ER; member of DUP240 gene family; PRM8 has a
paralog, PRM9, that arose from a segmental duplication"
/codon_start=1
/product="pheromone-regulated DUP240 family protein PRM8"
/protein_id="NP_011462.1"
/db_xref="GeneID:852828"
/db_xref="SGD:S000003021"
/translation="MQTPSENTNAKSDSLDEPGAYLIEENVALPKDIFHSYLSYWIYE
AAHCTPVMLLSLVIGVLISIIILFHDNENCVGVSVGFLLIFSGILVIVLILRFGPQIS
DEDFKCKLLMEIITRKPTVKGKEWRTITYKMNQYLFDNDLWNTPYYFYRDEDCHRYFL
SLIKGRTFKKQKESSASNVKDAQSNDETAGTPNEAAESSSFSAGPNFIKLLTKAAEIE
QQFQKEYWRQEYPGVDEFF"
gene <403687..>404391
/gene="MST27"
/locus_tag="YGL051W"
/db_xref="GeneID:852830"
mRNA <403687..>404391
/gene="MST27"
/locus_tag="YGL051W"
/product="DUP240 family protein MST27"
/transcript_id="NM_001180916.1"
/db_xref="GeneID:852830"
CDS 403687..404391
/gene="MST27"
/locus_tag="YGL051W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762|PMID:12925749]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:12925749]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:12925749]"
/experiment="EXISTENCE:genetic interaction:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:12925749]"
/experiment="EXISTENCE:genetic interaction:GO:0006890
retrograde vesicle-mediated transport, Golgi to
endoplasmic reticulum [PMID:12925749]"
/experiment="EXISTENCE:mutant phenotype:GO:0005783
endoplasmic reticulum [PMID:12101299]"
/experiment="EXISTENCE:mutant phenotype:GO:0005886 plasma
membrane [PMID:12101299]"
/note="Putative integral membrane protein, involved in
vesicle formation; forms complex with Mst28p; member of
DUP240 gene family; binds COPI and COPII vesicles; MST27
has a paralog, MST28, that arose from a segmental
duplication"
/codon_start=1
/product="DUP240 family protein MST27"
/protein_id="NP_011464.1"
/db_xref="GeneID:852830"
/db_xref="SGD:S000003019"
/translation="MQTPLESTDVKLDTLNEPSAHLIEKNVALPKDIFRSYLSYWIYE
IARYTPVMILSLVIGVLVLLIIFFNDNEACVFNSAYYAYLSLVVLLIILGDGNPKLVS
RRNFRTELLVDVITRKPAVEGKEWRIITYNMNQYLFNHGQWHTPYYFYSDEDCYRYFL
RLVEGVTPKKQTATSIGNSPVTAKPEDAIESASPSSRLNYRNFLLKAAEIERQAQENY
WRRRHPNIDALLKKTE"
repeat_region 405004..405145
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006945"
repeat_region 405146..405207
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006946"
repeat_region complement(405208..405453)
/note="Ty3 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006938"
gene 405470..405541
/locus_tag="YNCG0017W"
/db_xref="GeneID:852831"
tRNA 405470..405541
/locus_tag="YNCG0017W"
/product="tRNA-Arg"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE
analysis; one of 11 nuclear tRNA genes containing the
tDNA-anticodon UCU (converted to mcm5-UCU in the mature
tRNA), decodes AGA codons into arginine, one of 19 nuclear
tRNAs for arginine"
/db_xref="GeneID:852831"
/db_xref="SGD:S000006711"
gene <405776..>406597
/gene="TYW3"
/locus_tag="YGL050W"
/db_xref="GeneID:852832"
mRNA <405776..>406597
/gene="TYW3"
/locus_tag="YGL050W"
/product="tRNA methyltransferase TYW3"
/transcript_id="NM_001180915.3"
/db_xref="GeneID:852832"
CDS 405776..406597
/gene="TYW3"
/locus_tag="YGL050W"
/EC_number="2.1.1.282"
/experiment="EXISTENCE:direct assay:GO:0008175 tRNA
methyltransferase activity [PMID:16642040]"
/experiment="EXISTENCE:direct assay:GO:0030488 tRNA
methylation [PMID:16642040]"
/experiment="EXISTENCE:mutant phenotype:GO:0008175 tRNA
methyltransferase activity [PMID:16642040]"
/experiment="EXISTENCE:mutant phenotype:GO:0030488 tRNA
methylation [PMID:16642040]"
/experiment="EXISTENCE:mutant phenotype:GO:0031591
wybutosine biosynthetic process
[PMID:16642040|PMID:17150819]"
/note="tRNA methyltransferase required for synthesis of
wybutosine; a modified guanosine found at the 3'-position
adjacent to the anticodon of phenylalanine tRNA which
supports reading frame maintenance by stabilizing
codon-anticodon interactions"
/codon_start=1
/product="tRNA methyltransferase TYW3"
/protein_id="NP_011465.3"
/db_xref="GeneID:852832"
/db_xref="SGD:S000003018"
/translation="MAAQNAFEQKKRAILNEIDSTQPDLSPKGTIDELCLPIIDLINA
SADMVTTSSCSGRVSVFLEGTKSYNGEVKIGGKGQGGKWLYVTHDREKVIGWLDELKS
KSEFSFELSGKEIPTEKVTGSIRYILYKYEPFILHVKCRDFQAASKLYNTAMSCGFRE
SGIGSNNLVAIRINIKLDVPLGYLDETSGTLKFFVTPEYVSVLDSLSLSKFDENTRKM
QALYDRIEKELINCAPDVNSKVNITPIETKEERRERKKREGMERQRQLKSPQNVL"
gene complement(<406860..>409604)
/gene="TIF4632"
/locus_tag="YGL049C"
/db_xref="GeneID:852833"
mRNA complement(<406860..>409604)
/gene="TIF4632"
/locus_tag="YGL049C"
/product="translation initiation factor eIF4G"
/transcript_id="NM_001180914.1"
/db_xref="GeneID:852833"
CDS complement(406860..409604)
/gene="TIF4632"
/locus_tag="YGL049C"
/experiment="EXISTENCE:direct assay:GO:0000932 P-body
[PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:22851688|PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0003743 translation
initiation factor activity [PMID:22851688]"
/experiment="EXISTENCE:direct assay:GO:0006413
translational initiation [PMID:22851688]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule
[PMID:17908917|PMID:19470581|PMID:18981231|PMID:26777405]"
/experiment="EXISTENCE:direct assay:GO:0031369 translation
initiation factor binding [PMID:12861028]"
/experiment="EXISTENCE:mutant phenotype:GO:0003743
translation initiation factor activity [PMID:22851688]"
/experiment="EXISTENCE:mutant phenotype:GO:0006413
translational initiation [PMID:22851688]"
/experiment="EXISTENCE:mutant phenotype:GO:0016281
eukaryotic translation initiation factor 4F complex
[PMID:8336723]"
/experiment="EXISTENCE:mutant phenotype:GO:0031369
translation initiation factor binding [PMID:22851688]"
/experiment="EXISTENCE:mutant phenotype:GO:0034063 stress
granule assembly [PMID:18981231]"
/experiment="EXISTENCE:mutant phenotype:GO:0042149
cellular response to glucose starvation [PMID:18981231]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:12861028]"
/experiment="EXISTENCE:physical interaction:GO:0031369
translation initiation factor binding [PMID:22851688]"
/note="Translation initiation factor eIF4G and scaffold
protein; subunit of the mRNA cap-binding protein complex
(eIF4F) with eIF4E (Cdc33p) and eIF4A (Tif1p/Tif2p);
proposed to stimulate the ATPase and helicase activities
of eIF4A synergistically with eIF4G based on sequence
similarity with Tif4631p; proposed to be required with
eIF4E for Ded1p-mediated stimulation of mRNA recruitment
during 48S preinitiation complex (PIC) assembly;
associated with Pab1p-mediated poly(A)-tail stimulated,
translation"
/codon_start=1
/product="translation initiation factor eIF4G"
/protein_id="NP_011466.1"
/db_xref="GeneID:852833"
/db_xref="SGD:S000003017"
/translation="MTDQRGPPPPHPQQANGYKKFPPHDNQYSGANNSQPNNHYNENL
YSAREPHNNKQYQSKNGKYGTNKYNNRNNSQGNAQYYNNRFNNGYRLNNNDYNPAMLP
GMQWPANYYAPQMYYIPQQMVPVASPPYTHQPLNTNPEPPSTPKTTKIEITTKTGERL
NLKKFHEEKKASKGEEKNDGVEQKSKSGTPFEKEATPVLPANEAVKDTLTETSNEKST
SEAENTKRLFLEQVRLRKAAMERKKNGLISETEKKQETSNHDNTDTTKPNSVIESEPI
KEAPKPTGEANEVVIDGKSGASVKTPQHVTGSVTKSVTFNEPENESSSQDVDELVKDD
DTTEISDTTGGKTVNKSDDETINSVITTEENTVKETEPSTSDIEMPTVSQLLETLGKA
QPISDIYEFAYPENVERPDIKYKKPSVKYTYGPTFLLQFKDKLKFRPDPAWVEAVSSK
IVIPPHIARNKPKDSGRFGGDFRSPSMRGMDHTSSSRVSSKRRSKRMGDDRRSNRGYT
SRKDREKAAEKAEEQAPKEEIAPLVPSANRWIPKSRVKKTEKKLAPDGKTELFDKEEV
ERKMKSLLNKLTLEMFDSISSEILDIANQSKWEDDGETLKIVIEQIFHKACDEPHWSS
MYAQLCGKVVKDLDPNIKDKENEGKNGPKLVLHYLVARCHEEFEKGWADKLPAGEDGN
PLEPEMMSDEYYIAAAAKRRGLGLVRFIGYLYCLNLLTGKMMFECFRRLMKDLNNDPS
EETLESVIELLNTVGEQFEHDKFVTPQATLEGSVLLDNLFMLLQHIIDGGTISNRIKF
KLIDVKELREIKHWNSAKKDAGPKTIQQIHQEEEQLRQKKNSQRSNSRFNNHNQSNSN
RYSSNRRNMQNTQRDSFASTKTGSFRNNQRNARKVEEVSQAPRANMFDALMNNDGDSD
"
gene complement(<410069..>411286)
/gene="RPT6"
/locus_tag="YGL048C"
/gene_synonym="CIM3; CRL3; SCB68; SUG1"
/db_xref="GeneID:852834"
mRNA complement(<410069..>411286)
/gene="RPT6"
/locus_tag="YGL048C"
/gene_synonym="CIM3; CRL3; SCB68; SUG1"
/product="proteasome regulatory particle base subunit
RPT6"
/transcript_id="NM_001180913.1"
/db_xref="GeneID:852834"
CDS complement(410069..411286)
/gene="RPT6"
/locus_tag="YGL048C"
/gene_synonym="CIM3; CRL3; SCB68; SUG1"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10419517]"
/experiment="EXISTENCE:direct assay:GO:0008540 proteasome
regulatory particle, base subcomplex
[PMID:9741626|PMID:11742986]"
/experiment="EXISTENCE:direct assay:GO:0019904 protein
domain specific binding [PMID:7870180]"
/experiment="EXISTENCE:direct assay:GO:0034515 proteasome
storage granule [PMID:18504300]"
/experiment="EXISTENCE:genetic interaction:GO:0006289
nucleotide-excision repair [PMID:11410533]"
/experiment="EXISTENCE:genetic interaction:GO:0045899
positive regulation of RNA polymerase II transcription
preinitiation complex assembly [PMID:16269334]"
/experiment="EXISTENCE:mutant phenotype:GO:0006338
chromatin remodeling [PMID:20855529]"
/experiment="EXISTENCE:mutant phenotype:GO:0032968
positive regulation of transcription elongation by RNA
polymerase II [PMID:11389845]"
/experiment="EXISTENCE:mutant phenotype:GO:0045899
positive regulation of RNA polymerase II transcription
preinitiation complex assembly [PMID:19843524]"
/experiment="EXISTENCE:mutant phenotype:GO:0070651
nonfunctional rRNA decay [PMID:22505030]"
/experiment="EXISTENCE:mutant phenotype:GO:0070682
proteasome regulatory particle assembly [PMID:19412160]"
/experiment="EXISTENCE:physical interaction:GO:0031625
ubiquitin protein ligase binding
[PMID:10688918|PMID:12447385]"
/experiment="EXISTENCE:physical interaction:GO:0043161
proteasome-mediated ubiquitin-dependent protein catabolic
process [PMID:10688918]"
/note="ATPase of the 19S regulatory particle of the 26S
proteasome; one of six ATPases of the regulatory particle;
involved in the degradation of ubiquitinated substrates;
bound by ubiquitin-protein ligases Ubr1p and Ufd4p;
localized mainly to the nucleus throughout the cell cycle;
protein abundance increases in response to DNA replication
stress"
/codon_start=1
/product="proteasome regulatory particle base subunit
RPT6"
/protein_id="NP_011467.1"
/db_xref="GeneID:852834"
/db_xref="SGD:S000003016"
/translation="MTAAVTSSNIVLETHESGIKPYFEQKIQETELKIRSKTENVRRL
EAQRNALNDKVRFIKDELRLLQEPGSYVGEVIKIVSDKKVLVKVQPEGKYIVDVAKDI
NVKDLKASQRVCLRSDSYMLHKVLENKADPLVSLMMVEKVPDSTYDMVGGLTKQIKEI
KEVIELPVKHPELFESLGIAQPKGVILYGPPGTGKTLLARAVAHHTDCKFIRVSGAEL
VQKYIGEGSRMVRELFVMAREHAPSIIFMDEIDSIGSTRVEGSGGGDSEVQRTMLELL
NQLDGFETSKNIKIIMATNRLDILDPALLRPGRIDRKIEFPPPSVAARAEILRIHSRK
MNLTRGINLRKVAEKMNGCSGADVKGVCTEAGMYALRERRIHVTQEDFELAVGKVMNK
NQETAISVAKLFK"
gene <411552..>412160
/gene="ALG13"
/locus_tag="YGL047W"
/db_xref="GeneID:852835"
mRNA <411552..>412160
/gene="ALG13"
/locus_tag="YGL047W"
/product="N-acetylglucosaminyldiphosphodolichol
N-acetylglucosaminyltransferase catalytic subunit ALG13"
/transcript_id="NM_001180912.1"
/db_xref="GeneID:852835"
CDS 411552..412160
/gene="ALG13"
/locus_tag="YGL047W"
/EC_number="2.4.1.141"
/experiment="EXISTENCE:direct assay:GO:0004577
N-acetylglucosaminyldiphosphodolichol
N-acetylglucosaminyltransferase activity [PMID:16100113]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14690591]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14690591|PMID:16100110]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762|PMID:16100113]"
/experiment="EXISTENCE:direct assay:GO:0006488
dolichol-linked oligosaccharide biosynthetic process
[PMID:16100113]"
/experiment="EXISTENCE:mutant phenotype:GO:0004577
N-acetylglucosaminyldiphosphodolichol
N-acetylglucosaminyltransferase activity
[PMID:15615718|PMID:16100113]"
/experiment="EXISTENCE:mutant phenotype:GO:0006488
dolichol-linked oligosaccharide biosynthetic process
[PMID:16100113|PMID:15615718]"
/experiment="EXISTENCE:physical interaction:GO:0043541
UDP-N-acetylglucosamine transferase complex
[PMID:16100110|PMID:16100113]"
/note="Catalytic component of UDP-GlcNAc transferase;
required for the second step of dolichyl-linked
oligosaccharide synthesis; anchored to the ER membrane via
interaction with Alg14p; similar to bacterial and human
glycosyltransferases; protein abundance increases in
response to DNA replication stress; both human homologs
ALG13 and ALG14 are required to complement yeast alg13
mutant"
/codon_start=1
/product="N-acetylglucosaminyldiphosphodolichol
N-acetylglucosaminyltransferase catalytic subunit ALG13"
/protein_id="NP_011468.1"
/db_xref="GeneID:852835"
/db_xref="SGD:S000003015"
/translation="MGIIEEKALFVTCGATVPFPKLVSCVLSDEFCQELIQYGFVRLI
IQFGRNYSSEFEHLVQERGGQRESQKIPIDQFGCGDTARQYVLMNGKLKVIGFDFSTK
MQSIIRDYSDLVISHAGTGSILDSLRLNKPLIVCVNDSLMDNHQQQIADKFVELGYVW
SCAPTETGLIAGLRASQTEKLKPFPVSHNPSFERLLVETIYS"
gene complement(412294..412367)
/locus_tag="YNCG0018C"
/db_xref="GeneID:852836"
tRNA complement(412294..412367)
/locus_tag="YNCG0018C"
/product="tRNA-Val"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Valine tRNA (tRNA-Val), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:852836"
/db_xref="SGD:S000006755"
repeat_region 412456..412725
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006947"
gene <414102..>415730
/gene="RIM8"
/locus_tag="YGL045W"
/gene_synonym="ART9; PAL3; YGL046W"
/db_xref="GeneID:852837"
mRNA <414102..>415730
/gene="RIM8"
/locus_tag="YGL045W"
/gene_synonym="ART9; PAL3; YGL046W"
/product="Rim8p"
/transcript_id="NM_001180910.3"
/db_xref="GeneID:852837"
CDS 414102..415730
/gene="RIM8"
/locus_tag="YGL045W"
/gene_synonym="ART9; PAL3; YGL046W"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0009898 cytoplasmic
side of plasma membrane [PMID:20028738]"
/experiment="EXISTENCE:mutant phenotype:GO:0070086
ubiquitin-dependent endocytosis [PMID:31658248]"
/experiment="EXISTENCE:mutant phenotype:GO:0071230
cellular response to amino acid stimulus [PMID:31658248]"
/note="Protein involved in proteolytic activation of
Rim101p; part of response to alkaline pH; interacts with
ESCRT-1 subunits Stp22p and Vps28p; essential for
anaerobic growth; member of the arrestin-related
trafficking adaptor family"
/codon_start=1
/product="Rim8p"
/protein_id="NP_011470.4"
/db_xref="GeneID:852837"
/db_xref="SGD:S000003013"
/translation="MSLLRLWNKESRAPSKIKSHGIVGSYGNSMLAHNNVKQFRIDID
EPHRVWKPNESITGEAVIDIKRDITNVAIKLSLVCEVRVKTGNSPTSKNKRIEKTLEK
STFLYGQDYVKTAFSAKEKKPHVDKTTILNGLSKGEHRFPFRIRIPRGRGMLSSIKFE
RGSITYFLSCTLESLNNINGLKKPEARCEREFAVIVPLDVSRLPKPKTKTVVLQSASM
VQNKKNKSTEDESSSYTQLTQKSTTSNSSSSSVNSKTSPLPNKTVTISVDIPQAGFMI
GEIIPIDVKIDHYKPFYAPAGLTTTLVRICRVGGAGKDDPMETFRKDICQSISPIYIN
PETLQFQSRVYLKVPLDAFSTLTTVGKFFSFQYYIEVMVNLSKKNVVYTESNRIIGTP
IGEQNGLGVENNINRIQRKMLRMVNPETLENDSEGYESSIFFKDMVNVEKLKRLRNVT
GMSIETVIGTTRSEQQQSDASIPSQSSITAPQNSPSNLRDWLAPLNAYDSDDVPVPKY
SPNDKVSVPSEDKQELEQKRLQQLESDPPPCDDY"
gene complement(<416146..>417036)
/gene="RNA15"
/locus_tag="YGL044C"
/db_xref="GeneID:852838"
mRNA complement(<416146..>417036)
/gene="RNA15"
/locus_tag="YGL044C"
/product="Rna15p"
/transcript_id="NM_001180909.1"
/db_xref="GeneID:852838"
CDS complement(416146..417036)
/gene="RNA15"
/locus_tag="YGL044C"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:17684230]"
/experiment="EXISTENCE:direct assay:GO:0006397 mRNA
processing [PMID:11344258]"
/experiment="EXISTENCE:physical interaction:GO:0005848
mRNA cleavage stimulating factor complex [PMID:11344258]"
/note="Component of the cleavage and polyadenylation
factor I (CF I); CF 1, composed of the CF 1A complex
(Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in
cleavage and polyadenylation of mRNA 3' ends; interacts
with the A-rich polyadenylation signal in complex with
Rna14p and Hrp1p; mutant displays reduced transcription
elongation in the G-less-based run-on (GLRO) assay;
required for gene looping and maintenance of genome
stability"
/codon_start=1
/product="Rna15p"
/protein_id="NP_011471.1"
/db_xref="GeneID:852838"
/db_xref="SGD:S000003012"
/translation="MNRQSGVNAGVQNNPPSRVVYLGSIPYDQTEEQILDLCSNVGPV
INLKMMFDPQTGRSKGYAFIEFRDLESSASAVRNLNGYQLGSRFLKCGYSSNSDISGV
SQQQQQQYNNINGNNNNNGNNNNNSNGPDFQNSGNANFLSQKFPELPSGIDVNINMTT
PAMMISSELAKKPKEVQLKFLQKFQEWTRAHPEDAVSLLELCPQLSFVTAELLLTNGI
CKVDDLIPLASRPQEEASATNNNSVNEVVDPAVLNKQKELLKQVLQLNDSQISILPDD
ERMAIWDLKQKALRGEFGAF"
gene <417483..>418412
/gene="DST1"
/locus_tag="YGL043W"
/gene_synonym="PPR2"
/db_xref="GeneID:852839"
mRNA <417483..>418412
/gene="DST1"
/locus_tag="YGL043W"
/gene_synonym="PPR2"
/product="transcription elongation factor DST1"
/transcript_id="NM_001180908.1"
/db_xref="GeneID:852839"
CDS 417483..418412
/gene="DST1"
/locus_tag="YGL043W"
/gene_synonym="PPR2"
/experiment="EXISTENCE:direct assay:GO:0000977 RNA
polymerase II transcription regulatory region
sequence-specific DNA binding [PMID:15767671]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9334234]"
/experiment="EXISTENCE:direct assay:GO:0006362
transcription elongation by RNA polymerase I
[PMID:7002153]"
/experiment="EXISTENCE:direct assay:GO:0006367
transcription initiation at RNA polymerase II promoter
[PMID:17901206]"
/experiment="EXISTENCE:direct assay:GO:0006368
transcription elongation by RNA polymerase II
[PMID:8288647|PMID:9334234|PMID:7020755|PMID:7002153]"
/experiment="EXISTENCE:direct assay:GO:0006383
transcription by RNA polymerase III [PMID:18628399]"
/experiment="EXISTENCE:direct assay:GO:0031564
transcription antitermination
[PMID:9169440|PMID:9037112|PMID:8636112]"
/experiment="EXISTENCE:direct assay:GO:0032968 positive
regulation of transcription elongation by RNA polymerase
II [PMID:8876173]"
/experiment="EXISTENCE:direct assay:GO:0045899 positive
regulation of RNA polymerase II transcription
preinitiation complex assembly [PMID:17913884]"
/experiment="EXISTENCE:genetic interaction:GO:0001193
maintenance of transcriptional fidelity during
transcription elongation by RNA polymerase II
[PMID:17535246]"
/experiment="EXISTENCE:genetic interaction:GO:0006367
transcription initiation at RNA polymerase II promoter
[PMID:17901206]"
/experiment="EXISTENCE:genetic interaction:GO:0031440
regulation of mRNA 3'-end processing [PMID:15531585]"
/experiment="EXISTENCE:mutant phenotype:GO:0001139 RNA
polymerase II complex recruiting activity [PMID:15767671]"
/experiment="EXISTENCE:mutant phenotype:GO:0001193
maintenance of transcriptional fidelity during
transcription elongation by RNA polymerase II
[PMID:16492753|PMID:14531857]"
/experiment="EXISTENCE:mutant phenotype:GO:0006367
transcription initiation at RNA polymerase II promoter
[PMID:17901206|PMID:15767671]"
/experiment="EXISTENCE:mutant phenotype:GO:0006368
transcription elongation by RNA polymerase II
[PMID:8288647]"
/experiment="EXISTENCE:mutant phenotype:GO:0031440
regulation of mRNA 3'-end processing [PMID:15531585]"
/experiment="EXISTENCE:mutant phenotype:GO:0042797 tRNA
transcription by RNA polymerase III [PMID:18628399]"
/experiment="EXISTENCE:mutant phenotype:GO:0045899
positive regulation of RNA polymerase II transcription
preinitiation complex assembly [PMID:17913884]"
/experiment="EXISTENCE:physical interaction:GO:0000993 RNA
polymerase II complex binding [PMID:6985606|PMID:8876173]"
/note="General transcription elongation factor TFIIS;
enables RNA polymerase II to read through blocks to
elongation by stimulating cleavage of nascent transcripts
stalled at transcription arrest sites; maintains RNAPII
elongation activity on ribosomal protein genes during
conditions of transcriptional stress"
/codon_start=1
/product="transcription elongation factor DST1"
/protein_id="NP_011472.1"
/db_xref="GeneID:852839"
/db_xref="SGD:S000003011"
/translation="MDSKEVLVHVKNLEKNKSNDAAVLEILHVLDKEFVPTEKLLRET
KVGVEVNKFKKSTNVEISKLVKKMISSWKDAINKNKRSRQAQQHHQDHAPGNAEDKTT
VGESVNGVQQPASSQSDAMKQDKYVSTKPRNSKNDGVDTAIYHHKLRDQVLKALYDVL
AKESEHPPQSILHTAKAIESEMNKVNNCDTNEAAYKARYRIIYSNVISKNNPDLKHKI
ANGDITPEFLATCDAKDLAPAPLKQKIEEIAKQNLYNAQGATIERSVTDRFTCGKCKE
KKVSYYQLQTRSADEPLTTFCTCEACGNRWKFS"
gene complement(<418704..>418886)
/locus_tag="YGL041C-B"
/db_xref="GeneID:1466454"
mRNA complement(<418704..>418886)
/locus_tag="YGL041C-B"
/product="uncharacterized protein"
/transcript_id="NM_001184550.1"
/db_xref="GeneID:1466454"
CDS complement(418704..418886)
/locus_tag="YGL041C-B"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/note="hypothetical protein; identified by fungal homology
and RT-PCR; SWAT-GFP and mCherry fusion proteins localize
to the endoplasmic reticulum"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878074.1"
/db_xref="GeneID:1466454"
/db_xref="SGD:S000028548"
/translation="MFDSSIERVTLELCFHITLSIMCGCSIYFLLLVFILTFYSSVLL
HLKLYFFSSDRAIFNA"
gene <418825..>419289
/gene="DPC13"
/locus_tag="YGL041W-A"
/db_xref="GeneID:5814844"
mRNA <418825..>419289
/gene="DPC13"
/locus_tag="YGL041W-A"
/product="Dpc13p"
/transcript_id="NM_001184647.4"
/db_xref="GeneID:5814844"
CDS 418825..419289
/gene="DPC13"
/locus_tag="YGL041W-A"
/note="Putative mitochondrial hypothetical protein;
conserved in fungi; identified by expression profiling and
mass spectrometry"
/codon_start=1
/product="Dpc13p"
/protein_id="NP_001106172.4"
/db_xref="GeneID:5814844"
/db_xref="SGD:S000028826"
/translation="MLRVIWKHSSRVTRSIELSNISTTNHTRSLRRLSWISPRRFYAQ
SWDDRQPNDKIDAHIKVQKLMDQINSRPNVLEKLEKVSNIMIEKKLVNLDGTSANEEN
TMKPWQMIKILMDRDLRHAMKEFKLELEKSGIQLGPEQLAPLMTVLGLEKKK"
gene complement(<419527..>420555)
/gene="HEM2"
/locus_tag="YGL040C"
/gene_synonym="OLE4; SLU1"
/db_xref="GeneID:852842"
mRNA complement(<419527..>420555)
/gene="HEM2"
/locus_tag="YGL040C"
/gene_synonym="OLE4; SLU1"
/product="porphobilinogen synthase HEM2"
/transcript_id="NM_001180905.1"
/db_xref="GeneID:852842"
CDS complement(419527..420555)
/gene="HEM2"
/locus_tag="YGL040C"
/gene_synonym="OLE4; SLU1"
/EC_number="4.2.1.24"
/experiment="EXISTENCE:direct assay:GO:0004655
porphobilinogen synthase activity [PMID:2204246]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008270 zinc ion
binding [PMID:8973546]"
/experiment="EXISTENCE:mutant phenotype:GO:0004655
porphobilinogen synthase activity
[PMID:323256|PMID:2445751]"
/experiment="EXISTENCE:mutant phenotype:GO:0006783 heme
biosynthetic process [PMID:14559249|PMID:323256]"
/note="Aminolevulinate dehydratase; a homo-octameric
enzyme, catalyzes the conversion of 5-aminolevulinate to
porphobilinogen, the second step in heme biosynthesis;
enzymatic activity is zinc-dependent; localizes to the
cytoplasm and nucleus; human homolog ALAD can complement
yeast hem2 mutant"
/codon_start=1
/product="porphobilinogen synthase HEM2"
/protein_id="NP_011475.1"
/db_xref="GeneID:852842"
/db_xref="SGD:S000003008"
/translation="MHTAEFLETEPTEISSVLAGGYNHPLLRQWQSERQLTKNMLIFP
LFISDNPDDFTEIDSLPNINRIGVNRLKDYLKPLVAKGLRSVILFGVPLIPGTKDPVG
TAADDPAGPVIQGIKFIREYFPELYIICDVCLCEYTSHGHCGVLYDDGTINRERSVSR
LAAVAVNYAKAGAHCVAPSDMIDGRIRDIKRGLINANLAHKTFVLSYAAKFSGNLYGP
FRDAACSAPSNGDRKCYQLPPAGRGLARRALERDMSEGADGIIVKPSTFYLDIMRDAS
EICKDLPICAYHVSGEYAMLHAAAEKGVVDLKTIAFESHQGFLRAGARLIITYLAPEF
LDWLDEEN"
rep_origin 421171..421279
/note="ARS718; Autonomously Replicating Sequence"
/db_xref="SGD:S000118458"
gene 423092..423205
/gene="SUP54"
/locus_tag="YNCG0019W"
/db_xref="GeneID:852843"
tRNA join(423092..423129,423162..423205)
/gene="SUP54"
/locus_tag="YNCG0019W"
/product="tRNA-Leu"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE
analysis; can mutate to suppress amber nonsense mutations"
/db_xref="GeneID:852843"
/db_xref="SGD:S000006640"
gene <423961..>425007
/locus_tag="YGL039W"
/db_xref="GeneID:852844"
mRNA <423961..>425007
/locus_tag="YGL039W"
/product="carbonyl reductase (NADPH-dependent)"
/transcript_id="NM_001180904.1"
/db_xref="GeneID:852844"
CDS 423961..425007
/locus_tag="YGL039W"
/experiment="EXISTENCE:direct assay:GO:0004029 aldehyde
dehydrogenase (NAD+) activity [PMID:25656103]"
/experiment="EXISTENCE:direct assay:GO:0004090 carbonyl
reductase (NADPH) activity [PMID:18630881|PMID:19016485]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008152 metabolic
process [PMID:16268655]"
/experiment="EXISTENCE:direct assay:GO:0016614
oxidoreductase activity, acting on CH-OH group of donors
[PMID:14574691|PMID:16268655]"
/experiment="EXISTENCE:direct assay:GO:0042180 ketone
metabolic process [PMID:14574691|PMID:16268655]"
/experiment="EXISTENCE:mutant phenotype:GO:1901426
response to furfural [PMID:25656103]"
/note="Aldehyde reductase; reduces aliphatic aldehyde
substrates using NADH as cofactor; shown to reduce
carbonyl compounds to chiral alcohols"
/codon_start=1
/product="carbonyl reductase (NADPH-dependent)"
/protein_id="NP_011476.1"
/db_xref="GeneID:852844"
/db_xref="SGD:S000003007"
/translation="MTTEKTVVFVSGATGFIALHVVDDLLKTGYKVIGSGRSQEKNDG
LLKKFKSNPNLSMEIVEDIAAPNAFDKVFQKHGKEIKVVLHIASPVHFNTTDFEKDLL
IPAVNGTKSILEAIKNYAADTVEKVVITSSVAALASPGDMKDTSFVVNEESWNKDTWE
SCQANAVSAYCGSKKFAEKTAWDFLEENQSSIKFTLSTINPGFVFGPQLFADSLRNGI
NSSSAIIANLVSYKLGDNFYNYSGPFIDVRDVSKAHLLAFEKPECAGQRLFLCEDMFC
SQEALDILNEEFPQLKGKIATGEPGSGSTFLTKNCCKCDNRKTKNLLGFQFNKFRDCI
VDTASQLLEVQSKS"
gene complement(<425362..>426804)
/gene="OCH1"
/locus_tag="YGL038C"
/gene_synonym="LDB12; NGD29"
/db_xref="GeneID:852845"
mRNA complement(<425362..>426804)
/gene="OCH1"
/locus_tag="YGL038C"
/gene_synonym="LDB12; NGD29"
/product="initiation-specific
alpha-1,6-mannosyltransferase"
/transcript_id="NM_001180903.1"
/db_xref="GeneID:852845"
CDS complement(425362..426804)
/gene="OCH1"
/locus_tag="YGL038C"
/gene_synonym="LDB12; NGD29"
/EC_number="2.4.1.232"
/experiment="EXISTENCE:direct assay:GO:0000009
alpha-1,6-mannosyltransferase activity
[PMID:9276464|PMID:17042779]"
/experiment="EXISTENCE:direct assay:GO:0000137 Golgi cis
cisterna [PMID:1628616|PMID:10189369]"
/experiment="EXISTENCE:mutant phenotype:GO:0000009
alpha-1,6-mannosyltransferase activity [PMID:8253757]"
/experiment="EXISTENCE:mutant phenotype:GO:0006487 protein
N-linked glycosylation [PMID:7649302]"
/note="Mannosyltransferase of the cis-Golgi apparatus;
initiates the polymannose outer chain elongation of
N-linked oligosaccharides of glycoproteins"
/codon_start=1
/product="initiation-specific
alpha-1,6-mannosyltransferase"
/protein_id="NP_011477.1"
/db_xref="GeneID:852845"
/db_xref="SGD:S000003006"
/translation="MSRKLSHLIATRKSKTIVVTVLLIYSLLTFHLSNKRLLSQFYPS
KDDFKQTLLPTTSHSQDINLKKQITVNKKKNQLHNLRDQLSFAFPYDSQAPIPQRVWQ
TWKVGADDKNFPSSFRTYQKTWSGSYSPDYQYSLISDDSIIPFLENLYAPVPIVIQAF
KLMPGNILKADFLRYLLLFARGGIYSDMDTMLLKPIDSWPSQNKSWLNNIIDLNKPIP
YKNSKPSLLSSDEISHQPGLVIGIEADPDRDDWSEWYARRIQFCQWTIQAKPGHPILR
ELILNITATTLASVQNPGVPVSEMIDPRFEEDYNVNYRHKRRHDETYKHSELKNNKNV
DGSDIMNWTGPGIFSDIIFEYMNNVLRYNSDILLINPNLNKNDEEGSESATTPAKDVD
NDTLSKSTRKFYKKISESLQSSNSMPWEFFSFLKEPVIVDDVMVLPITSFSPDVGQMG
AQSSDDKMAFVKHMFSGSWKEDADKNAGHK"
gene complement(<427297..>427947)
/gene="PNC1"
/locus_tag="YGL037C"
/db_xref="GeneID:852846"
mRNA complement(<427297..>427947)
/gene="PNC1"
/locus_tag="YGL037C"
/product="nicotinamidase"
/transcript_id="NM_001180902.3"
/db_xref="GeneID:852846"
CDS complement(427297..427947)
/gene="PNC1"
/locus_tag="YGL037C"
/EC_number="3.5.1.19"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12736687]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:12736687|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:12736687]"
/experiment="EXISTENCE:mutant phenotype:GO:0000183 rDNA
heterochromatin formation [PMID:11901108]"
/experiment="EXISTENCE:mutant phenotype:GO:0008936
nicotinamidase activity [PMID:19381334]"
/experiment="EXISTENCE:mutant phenotype:GO:0019358
nicotinate nucleotide salvage [PMID:11901108]"
/experiment="EXISTENCE:mutant phenotype:GO:0031509
subtelomeric heterochromatin formation [PMID:11901108]"
/experiment="EXISTENCE:mutant phenotype:GO:1904524
negative regulation of DNA amplification [PMID:26195783]"
/note="Nicotinamidase that converts nicotinamide to
nicotinic acid; part of the NAD(+) salvage pathway;
required for life span extension by calorie restriction;
lacks a peroxisomal targeting signal but is imported into
peroxisomes via binding to Gpd1p; PNC1 expression responds
to all known stimuli that extend replicative life span;
protein increases in abundance and relative distribution
to cytoplasmic foci decreases upon DNA replication stress"
/codon_start=1
/product="nicotinamidase"
/protein_id="NP_011478.3"
/db_xref="GeneID:852846"
/db_xref="SGD:S000003005"
/translation="MKTLIVVDMQNDFISPLGSLTVPKGEELINPISDLMQDADRDWH
RIVVTRDWHPSRHISFAKNHKDKEPYSTYTYHSPRPGDDSTQEGILWPVHCVKNTWGS
QLVDQIMDQVVTKHIKIVDKGFLTDREYYSAFHDIWNFHKTDMNKYLEKHHTDEVYIV
GVALEYCVKATAISAAELGYKTTVLLDYTRPISDDPEVINKVKEELKAHNINVVDK"
gene <428601..>431330
/gene="VIR1"
/locus_tag="YGL036W"
/db_xref="GeneID:852847"
mRNA <428601..>431330
/gene="VIR1"
/locus_tag="YGL036W"
/product="Vir1p"
/transcript_id="NM_001180901.1"
/db_xref="GeneID:852847"
CDS 428601..431330
/gene="VIR1"
/locus_tag="YGL036W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0036396 RNA
N6-methyladenosine methyltransferase complex
[PMID:37603553]"
/experiment="EXISTENCE:mutant phenotype:GO:0051321 meiotic
cell cycle [PMID:36930734]"
/experiment="EXISTENCE:physical interaction:GO:0036396 RNA
N6-methyladenosine methyltransferase complex
[PMID:36930734]"
/note="Protein required for mRNA m6A methylation and
meiosis; subunit of the m6A methyltransferase (MIS)
complex; required for the stability of MIS complex
subunits and consistent with a scaffolding function;
non-essential gene required for pre-meiotic S-phase; may
be required to initiate the meiotic program, for
progression through meiotic divisions and for spore
formation; green fluorescent protein (GFP)-fusion protein
localizes to the cytoplasm; homolog of
VIRMA/virilizer/VIR"
/codon_start=1
/product="Vir1p"
/protein_id="NP_011479.1"
/db_xref="GeneID:852847"
/db_xref="SGD:S000003004"
/translation="MQKCAGHAPLVTAASRVSQDTVDALLQAILKAYHKLASIDSHIN
DPVEIAFKLINSFKYLPISGSSVKDFESELRELDVFSPLLQSAVTAANNSNIIWDLIA
VLFAYISIHKQLHPLILHNLNIWKDFMADNDEETATTTDGDSMNFGVLSLLSIVQNFE
EITPNLFEFLKLGLRSTLLKIWVAQWQRYDPSATNLINGDEKISSWITKDYQVDFFII
TSLASTSSLEVLPSHYFVYKISKRISHFPNLIDPKLYRSAISTIMENGISDNGGGENS
SDKIDPTDLSFHLQVLMEVIDHPELNYLQENRLILLLDIALNYLILVPTHCLHSNFGE
LGSTQSLASTLNIIQFLLSKFLINMGSISQLINQYNRKCITTNNINNNNINNNGVING
STNTTSTTTTTITNNNNNSNNSSISNNNRKIDWTQSYQTRYQIPYWFEDSILPPIPPI
SKSLFTFDKNLDHESDSIMIVNDVLRCLNLTILLISKLLRDYDDLKINPLIQSSDDHS
NEDNHVIIEQYMQLYLVPLFTSLLLAQQLKDRGQERDEGHKEKEENINLIGSSSVKKL
FSQLIFFSSLKLCENLVIKEKNLALYHLIKFATKVSLDDLILQKISINLLNHLFFHQI
RDGSDDDNLIKKLCLKNQLSFQALKDYITLWNDGSEVYNAFYKELFYEEQPKIKPIKL
TTSDLLKLFPEDVQFVISTPPNTITSASTSDNCTSSQSAAQKNIENFTTLSKYDVYSS
TSFIPSTSKNTNTNVSKQQQQPQNSTPCSSNRFLFNKSSLISQESNGSNNNSGTQGPG
SMNESYSLDNSFNTTNTNMTRQPTTLTRATDAMTTAPTTPIPYKNTSGSSNNNLWIES
PMTNFKGSTISKSTNKSKMVNTGKNYILGGHNKVKNNSRAQSIHIDDFENENN"
gene complement(<431548..>433062)
/gene="MIG1"
/locus_tag="YGL035C"
/gene_synonym="CAT4; SSN1; TDS22"
/db_xref="GeneID:852848"
mRNA complement(<431548..>433062)
/gene="MIG1"
/locus_tag="YGL035C"
/gene_synonym="CAT4; SSN1; TDS22"
/product="transcription factor MIG1"
/transcript_id="NM_001180900.1"
/db_xref="GeneID:852848"
CDS complement(431548..433062)
/gene="MIG1"
/locus_tag="YGL035C"
/gene_synonym="CAT4; SSN1; TDS22"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:12239282|PMID:8114729|PMID:9832517]"
/experiment="EXISTENCE:direct assay:GO:0001227 DNA-binding
transcription repressor activity, RNA polymerase
II-specific [PMID:7724528|PMID:9832517]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9285828|PMID:17237508|PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005641 nuclear
envelope lumen [PMID:17237508]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:17237508|PMID:11914276|PMID:9285828]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:19111667]"
/experiment="EXISTENCE:genetic interaction:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:9858577]"
/experiment="EXISTENCE:genetic interaction:GO:0001227
DNA-binding transcription repressor activity, RNA
polymerase II-specific [PMID:7724528|PMID:9832517]"
/experiment="EXISTENCE:genetic interaction:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:9832517]"
/experiment="EXISTENCE:genetic interaction:GO:1900436
positive regulation of filamentous growth of a population
of unicellular organisms in response to starvation
[PMID:22904036]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:9858577]"
/experiment="EXISTENCE:mutant phenotype:GO:0001227
DNA-binding transcription repressor activity, RNA
polymerase II-specific [PMID:9832517]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:9832517]"
/note="Transcription factor involved in glucose
repression; sequence specific DNA binding protein
containing two Cys2His2 zinc finger motifs; regulated by
the SNF1 kinase and the GLC7 phosphatase; regulates
filamentous growth along with Mig2p in response to glucose
depletion; activated in stochastic pulses of nuclear
localization, shuttling between cytosol and nucleus
depending on external glucose levels and its
phosphorylation state"
/codon_start=1
/product="transcription factor MIG1"
/protein_id="NP_011480.1"
/db_xref="GeneID:852848"
/db_xref="SGD:S000003003"
/translation="MQSPYPMTQVSNVDDGSLLKESKSKSKVAAKSEAPRPHACPICH
RAFHRLEHQTRHMRIHTGEKPHACDFPGCVKRFSRSDELTRHRRIHTNSHPRGKRGRK
KKVVGSPINSASSSATSIPDLNTANFSPPLPQQHLSPLIPIAIAPKENSSRSSTRKGR
KTKFEIGESGGNDPYMVSSPKTMAKIPVSVKPPPSLALNNMNYQTSSASTALSSLSNS
HSGSRLKLNALSSLQMMTPIASSAPRTVFIDGPEQKQLQQQQNSLSPRYSNTVILPRP
RSLTDFQGLNNANPNNNGSLRAQTQSSVQLKRPSSVLSLNDLLVGQRNTNESDSDFTT
GGEDEEDGLKDPSNSSIDNLEQDYLQEQSRKKSKTSTPTTMLSRSTSGTNLHTLGYVM
NQNHLHFSSSSPDFQKELNNRLLNVQQQQQEQHTLLQSQNTSNQSQNQNQNQMMASSS
SLSTTPLLLSPRVNMINTAISTQQTPISQSDSQVQELETLPPIRSLPLPFPHMD"
gene complement(<433214..>433579)
/locus_tag="YGL034C"
/db_xref="GeneID:852849"
mRNA complement(<433214..>433579)
/locus_tag="YGL034C"
/product="uncharacterized protein"
/transcript_id="NM_001348837.1"
/db_xref="GeneID:852849"
CDS complement(433214..433579)
/locus_tag="YGL034C"
/note="hypothetical protein; conserved among S. cerevisiae
strains; YGL034C is not an essential gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335778.1"
/db_xref="GeneID:852849"
/db_xref="SGD:S000003002"
/translation="MNVVAIIMASQETCSGRAPRETPEWRRPACLVASILVRALEFSW
LFSTCLLVAFDFARDCGLPPHSGKTWSDGKGPAALSFGKTNTKEATTNFYNRLDEKSG
EEQAEKRKENSRSWIIYLL"
gene <435625..>436401
/gene="HOP2"
/locus_tag="YGL033W"
/db_xref="GeneID:852850"
mRNA join(<435625..435679,435750..436312,436375..>436401)
/gene="HOP2"
/locus_tag="YGL033W"
/product="Hop2p"
/transcript_id="NM_001180898.2"
/db_xref="GeneID:852850"
CDS join(435625..435679,435750..436312,436375..436401)
/gene="HOP2"
/locus_tag="YGL033W"
/experiment="EXISTENCE:direct assay:GO:0000794 condensed
nuclear chromosome [PMID:9708739]"
/experiment="EXISTENCE:direct assay:GO:0003690
double-stranded DNA binding [PMID:15249670]"
/experiment="EXISTENCE:mutant phenotype:GO:0007129
homologous chromosome pairing at meiosis [PMID:9708739]"
/experiment="EXISTENCE:mutant phenotype:GO:0007131
reciprocal meiotic recombination [PMID:16581767]"
/note="Meiosis-specific protein that localizes to
chromosomes; prevents synapsis between nonhomologous
chromosomes and ensures synapsis between homologs; forms
complex with Mnd1p to promote homolog pairing and meiotic
double-strand break repair; heterodimer of Hop2p-Mnd1p
stimulates the Dmc1p-mediated strand invasion"
/codon_start=1
/product="Hop2p"
/protein_id="NP_011482.3"
/db_xref="GeneID:852850"
/db_xref="SGD:S000003001"
/translation="MAPKKKSNDRAIQAKGSEAEQLIEDYLVSQYKPFSVNDIVQNLH
NKVTKTTATKALENLVNEKRIVSKTFGKIIIYSCNEQDTALPSNIDPSQFDFETVLQL
RNDLIELERDKSTAKDALDSVTKEPENEDLLTIIENEENELKKIESKLQSLQDDWDPA
NDEIVKRIMSEDTLLQKEITKRSKICKNLIATIKDSVCPKNMNEFLEEIGFEDI"
gene complement(<436570..>436833)
/gene="AGA2"
/locus_tag="YGL032C"
/db_xref="GeneID:852851"
mRNA complement(<436570..>436833)
/gene="AGA2"
/locus_tag="YGL032C"
/product="Aga2p"
/transcript_id="NM_001180897.3"
/db_xref="GeneID:852851"
CDS complement(436570..436833)
/gene="AGA2"
/locus_tag="YGL032C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0000752
agglutination involved in conjugation with cellular fusion
[PMID:1756718]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:1756718]"
/experiment="EXISTENCE:direct assay:GO:0050839 cell
adhesion molecule binding [PMID:11292808|PMID:7957044]"
/note="Adhesion subunit of a-agglutinin of a-cells;
C-terminal sequence acts as a ligand for alpha-agglutinin
(Sag1p) during agglutination, modified with O-linked
oligomannosyl chains, linked to anchorage subunit Aga1p
via two disulfide bonds"
/codon_start=1
/product="Aga2p"
/protein_id="NP_011483.3"
/db_xref="GeneID:852851"
/db_xref="SGD:S000003000"
/translation="MQLLRCFSIFSVIASVLAQELTTICEQIPSPTLESTPYSLSTTT
ILANGKAMQGVFEYYKSVTFVSNCGSHPSTTSKGSPINTQYVF"
gene complement(<437467..>437934)
/gene="RPL24A"
/locus_tag="YGL031C"
/gene_synonym="RPL30A"
/db_xref="GeneID:852852"
mRNA complement(<437467..>437934)
/gene="RPL24A"
/locus_tag="YGL031C"
/gene_synonym="RPL30A"
/product="60S ribosomal protein eL24 RPL24A"
/transcript_id="NM_001180896.1"
/db_xref="GeneID:852852"
CDS complement(437467..437934)
/gene="RPL24A"
/locus_tag="YGL031C"
/gene_synonym="RPL30A"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:6337137]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:11983894]"
/experiment="EXISTENCE:mutant phenotype:GO:0006413
translational initiation [PMID:30407570]"
/experiment="EXISTENCE:mutant phenotype:GO:0006414
translational elongation [PMID:30407570]"
/note="Ribosomal 60S subunit protein L24A; forms two
bridges within ribosome, stimulates translation initiation
and elongation; homologous to mammalian ribosomal protein
L24, no bacterial homolog; RPL24A has a paralog, RPL24B,
that arose from the whole genome duplication"
/codon_start=1
/product="60S ribosomal protein eL24 RPL24A"
/protein_id="NP_011484.1"
/db_xref="GeneID:852852"
/db_xref="SGD:S000002999"
/translation="MKVEIDSFSGAKIYPGRGTLFVRGDSKIFRFQNSKSASLFKQRK
NPRRIAWTVLFRKHHKKGITEEVAKKRSRKTVKAQRPITGASLDLIKERRSLKPEVRK
ANREEKLKANKEKKKAEKAARKAEKAKSAGTQSSKFSKQQAKGAFQKVAATSR"
gene <439091..>439638
/gene="RPL30"
/locus_tag="YGL030W"
/db_xref="GeneID:852853"
mRNA join(<439091..439093,439324..>439638)
/gene="RPL30"
/locus_tag="YGL030W"
/product="60S ribosomal protein eL30 RPL30"
/transcript_id="NM_001180895.1"
/db_xref="GeneID:852853"
CDS join(439091..439093,439324..439638)
/gene="RPL30"
/locus_tag="YGL030W"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:22096102]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:14970382]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:9121443]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11142377]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:22096102]"
/experiment="EXISTENCE:genetic interaction:GO:0030627
pre-mRNA 5'-splice site binding [PMID:18570876]"
/experiment="EXISTENCE:mutant phenotype:GO:0006364 rRNA
processing [PMID:9121443]"
/experiment="EXISTENCE:mutant phenotype:GO:0030627
pre-mRNA 5'-splice site binding [PMID:18570876]"
/experiment="EXISTENCE:mutant phenotype:GO:0048025
negative regulation of mRNA splicing, via spliceosome
[PMID:18570876|PMID:9121443]"
/note="Ribosomal 60S subunit protein L30; involved in
pre-rRNA processing in the nucleolus; autoregulates
splicing of its transcript; homologous to mammalian
ribosomal protein L30, no bacterial homolog"
/codon_start=1
/product="60S ribosomal protein eL30 RPL30"
/protein_id="NP_011485.1"
/db_xref="GeneID:852853"
/db_xref="SGD:S000002998"
/translation="MAPVKSQESINQKLALVIKSGKYTLGYKSTVKSLRQGKSKLIII
AANTPVLRKSELEYYAMLSKTKVYYFQGGNNELGTAVGKLFRVGVVSILEAGDSDILT
TLA"
gene <440063..>440425
/gene="CGR1"
/locus_tag="YGL029W"
/db_xref="GeneID:852854"
mRNA <440063..>440425
/gene="CGR1"
/locus_tag="YGL029W"
/product="Cgr1p"
/transcript_id="NM_001180894.1"
/db_xref="GeneID:852854"
CDS 440063..440425
/gene="CGR1"
/locus_tag="YGL029W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:11116400]"
/experiment="EXISTENCE:direct assay:GO:0006364 rRNA
processing [PMID:11932453]"
/experiment="EXISTENCE:direct assay:GO:0030687
preribosome, large subunit precursor [PMID:17443350]"
/experiment="EXISTENCE:mutant phenotype:GO:0042254
ribosome biogenesis [PMID:11116400]"
/note="Protein involved in nucleolar integrity and
processing of pre-rRNA; has a role in processing rRNA for
the 60S ribosome subunit; transcript is induced in
response to cytotoxic stress but not genotoxic stress;
relocalizes from nucleus to nucleolus upon DNA replication
stress"
/codon_start=1
/product="Cgr1p"
/protein_id="NP_011486.1"
/db_xref="GeneID:852854"
/db_xref="SGD:S000002997"
/translation="MVNETGESQKAAKGTPVSGKVWKAEKTPLRAKSRVVKNKKLTSW
ELKKQKRLEDKQFKERLKALKDEKEEARQAKITMLKERREKKEENERYERLAAKMHAK
KVERMRRREKRNKALKER"
gene complement(440716..440807)
/locus_tag="YNCG0020C"
/db_xref="GeneID:852855"
tRNA complement(join(440716..440751,440771..440807))
/locus_tag="YNCG0020C"
/product="tRNA-Phe"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:10024171]"
/note="Phenylalanine tRNA (tRNA-Phe), predicted by
tRNAscan-SE analysis"
/db_xref="GeneID:852855"
/db_xref="SGD:S000006565"
gene complement(<441281..>442909)
/gene="SCW11"
/locus_tag="YGL028C"
/db_xref="GeneID:852856"
mRNA complement(<441281..>442909)
/gene="SCW11"
/locus_tag="YGL028C"
/product="putative glucan endo-1,3-beta-D-glucosidase"
/transcript_id="NM_001180893.3"
/db_xref="GeneID:852856"
CDS complement(441281..442909)
/gene="SCW11"
/locus_tag="YGL028C"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:9748433]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="Cell wall protein with similarity to glucanases;
may play a role in conjugation during mating based on its
regulation by Ste12p"
/codon_start=1
/product="putative glucan endo-1,3-beta-D-glucosidase"
/protein_id="NP_011487.3"
/db_xref="GeneID:852856"
/db_xref="SGD:S000002996"
/translation="MISPISFLSSLLCLTYLTSALPILPKREVVTRVHTASTTNVVTD
FYSTTTEVVIAPTVEFLISDSVTFTTTLIPQGVNPTAEPTTTITKVLLKKAEMSTSSQ
PTSTLQPSTIPQSTSSFQAESTLQAVSTQQTAMSVSAGTSEDVQQLATTSTSISSSPS
PTTTSTSTQNIVSGVGEANVEGNTQSQHHSYQAAATSTLNQQTSTSIASQESTESTNT
PTSSSTSSSTSSSTSSSTSSSTSSSTSSSTSSSTSSSTSSTQETAATTSEGSSSSSAA
ITSSPKAIAYSPYNDDGSCKSADAVSSDLTLIKSKGISKIRVYGTDCNSFETVQPAAV
KLGIKINQGLYITSSGVDSIDDSVTTLIQYGQTNGWDVFDFITVGNEAINNGWCSVSD
LISKISSVKSKLSEAGYSGQITTSEPPVSFENNPDLCKKSDIDFVGINPHAYFDTSAS
AETAGTFVKGQVELIQGVCGTSNVFVTETGYPSSGIQNGGNIPSTANQITAVQNILNE
MDLDVTILSTYNDYWKAPGDYGIEQSFGVIEYFP"
gene complement(<443642..>446143)
/gene="CWH41"
/locus_tag="YGL027C"
/gene_synonym="DER7; GLS1"
/db_xref="GeneID:852857"
mRNA complement(<443642..>446143)
/gene="CWH41"
/locus_tag="YGL027C"
/gene_synonym="DER7; GLS1"
/product="mannosyl-oligosaccharide glucosidase"
/transcript_id="NM_001180892.1"
/db_xref="GeneID:852857"
CDS complement(443642..446143)
/gene="CWH41"
/locus_tag="YGL027C"
/gene_synonym="DER7; GLS1"
/EC_number="3.2.1.106"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0004573
Glc3Man9GlcNAc2 oligosaccharide glucosidase activity
[PMID:14680956]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:8576053]"
/experiment="EXISTENCE:genetic interaction:GO:0070880
fungal-type cell wall beta-glucan biosynthetic process
[PMID:9430631]"
/experiment="EXISTENCE:mutant phenotype:GO:0004573
Glc3Man9GlcNAc2 oligosaccharide glucosidase activity
[PMID:9430631]"
/experiment="EXISTENCE:mutant phenotype:GO:0006487 protein
N-linked glycosylation [PMID:9363442]"
/experiment="EXISTENCE:mutant phenotype:GO:0009272
fungal-type cell wall biogenesis [PMID:9430631]"
/note="Processing alpha glucosidase I; ER type II integral
membrane N-glycoprotein involved in assembly of cell wall
beta 1,6 glucan and asparagine-linked protein
glycosylation; has a role in ER protein quality control
and sensing of ER stress; ortholog of the human
mannosyl-oligosaccharide glucosidase MOGS, defects of
which are associated with congenital disorders of
glycosylation"
/codon_start=1
/product="mannosyl-oligosaccharide glucosidase"
/protein_id="NP_011488.1"
/db_xref="GeneID:852857"
/db_xref="SGD:S000002995"
/translation="MLISKSKMFKTFWILTSIVLLASATVDISKLQEFEEYQKFTNES
LLWAPYRSNCYFGMRPRYVHESPLIMGIMWFNSLSQDGLHSLRHFATPQDKLQKYGWE
VYDPRIGGKEVFIDEKNNLNLTVYFVKSKNGENWSVRVQGEPLDPKRPSTASVVLYFS
QNGGEIDGKSSLAMIGHDGPNDMKFFGYSKELGEYHLTVKDNFGHYFKNPEYETMEVA
PGSDCSKTSHLSLQIPDKEVWKARDVFQSLVSDSIRDILEKEETKQRPADLIPSVLTI
RNLYNFNPGNFHYIQKTFDLTKKDGFQFDITYNKLGTTQSISTREQVTELITWSLNEI
NARFDKQFSFGEGPDSIESVEVKRRFALETLSNLLGGIGYFYGNQLIDRETEFDESQF
TEIKLLNAKEEGPFELFTSVPSRGFFPRGFYWDEGFHLLQIMEYDFDLAFEILASWFE
MIEDDSGWIAREIILGNEARSKVPQEFQVQNPNIANPPTLLLAFSEMLSRAIENIGDF
NSDSYHQVMFNSRTAKFMTNNLEANPGLLTEYAKKIYPKLLKHYNWFRKSQTGLIDEY
EEILEDEGIWDKIHKNEVYRWVGRTFTHCLPSGMDDYPRAQPPDVAELNVDALAWVGV
MTRSMKQIAHVLKLTQDEQRYAQIEQEVVENLDLLHWSENDNCYCDISIDPEDDEIRE
FVCHEGYVSVLPFALKLIPKNSPKLEKVVALMSDPEKIFSDYGLLSLSRQDDYFGKDE
NYWRGPIWMNINYLCLDAMRYYYPEVILDVAGEASNAKKLYQSLKINLSNNIYKVWEE
QGYCYENYSPIDGHGTGAEHFTGWTALVVNILGRF"
gene complement(<446412..>448535)
/gene="TRP5"
/locus_tag="YGL026C"
/db_xref="GeneID:852858"
mRNA complement(<446412..>448535)
/gene="TRP5"
/locus_tag="YGL026C"
/product="tryptophan synthase TRP5"
/transcript_id="NM_001180891.1"
/db_xref="GeneID:852858"
CDS complement(446412..448535)
/gene="TRP5"
/locus_tag="YGL026C"
/EC_number="4.2.1.20"
/experiment="EXISTENCE:direct assay:GO:0004834 tryptophan
synthase activity [PMID:6342605|PMID:391563]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276|PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0000162
L-tryptophan biosynthetic process [PMID:10790693]"
/note="Tryptophan synthase; catalyzes the last step of
tryptophan biosynthesis; regulated by the general control
system of amino acid biosynthesis"
/codon_start=1
/product="tryptophan synthase TRP5"
/protein_id="NP_011489.1"
/db_xref="GeneID:852858"
/db_xref="SGD:S000002994"
/translation="MSEQLRQTFANAKKENRNALVTFMTAGYPTVKDTVPILKGFQDG
GVDIIELGMPFSDPIADGPTIQLSNTVALQNGVTLPQTLEMVSQARNEGVTVPIILMG
YYNPILNYGEERFIQDAAKAGANGFIIVDLPPEEALKVRNYINDNGLSLIPLVAPSTT
DERLELLSHIADSFVYVVSRMGTTGVQSSVASDLDELISRVRKYTKDTPLAVGFGVST
REHFQSVGSVADGVVIGSKIVTLCGDAPEGKRYDVAKEYVQGILNGAKHKVLSKDEFF
AFQKESLKSANVKKEILDEFDENHKHPIRFGDFGGQYVPEALHACLRELEKGFDEAVA
DPTFWEDFKSLYSYIGRPSSLHKAERLTEHCQGAQIWLKREDLNHTGSHKINNALAQV
LLAKRLGKKNVIAETGAGQHGVATATACAKFGLTCTVFMGAEDVRRQALNVFRMRILG
AKVIAVTNGTKTLRDATSEAFRFWVTNLKTTYYVVGSAIGPHPYPTLVRTFQSVIGKE
TKEQFAAMNNGKLPDAVVACVGGGSNSTGMFSPFEHDTSVKLLGVEAGGDGVDTKFHS
ATLTAGRPGVFHGVKTYVLQDSDGQVHDTHSVSAGLDYPGVGPELAYWKSTGRAQFIA
ATDAQALLGFKLLSQLEGIIPALESSHAVYGACELAKTMKPDQHLVINISGRGDKDVQ
SVAEVLPKLGPKIGWDLRFEEDPSA"
gene complement(<448764..>449957)
/gene="PGD1"
/locus_tag="YGL025C"
/gene_synonym="HRS1; MED3"
/db_xref="GeneID:852860"
mRNA complement(<448764..>449957)
/gene="PGD1"
/locus_tag="YGL025C"
/gene_synonym="HRS1; MED3"
/product="Pgd1p"
/transcript_id="NM_001180890.1"
/db_xref="GeneID:852860"
CDS complement(448764..449957)
/gene="PGD1"
/locus_tag="YGL025C"
/gene_synonym="HRS1; MED3"
/experiment="EXISTENCE:direct assay:GO:0000979 RNA
polymerase II core promoter sequence-specific DNA binding
[PMID:23447536]"
/experiment="EXISTENCE:direct assay:GO:0034605 cellular
response to heat [PMID:23447536]"
/experiment="EXISTENCE:direct assay:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:11073972]"
/experiment="EXISTENCE:direct assay:GO:0070847 core
mediator complex [PMID:9891034]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:10722672]"
/experiment="EXISTENCE:mutant phenotype:GO:0003713
transcription coactivator activity [PMID:15254252]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:12558798]"
/experiment="EXISTENCE:mutant phenotype:GO:0051123 RNA
polymerase II preinitiation complex assembly
[PMID:12482986]"
/experiment="EXISTENCE:physical interaction:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:10722672]"
/note="Subunit of the RNA polymerase II mediator complex;
associates with core polymerase subunits to form the RNA
polymerase II holoenzyme; essential for basal and
activated transcription; direct target of Cyc8p-Tup1p
transcriptional corepressor"
/codon_start=1
/product="Pgd1p"
/protein_id="NP_011490.2"
/db_xref="GeneID:852860"
/db_xref="SGD:S000002993"
/translation="MDSIIPAGVKLDDLQVILAKNENETRDKVCKQINEARDEILPLR
LQFNEFIQIMANIDQEGSKQADRMAKYLHIRDKILQLNDRFQTLSSHLEALQPLFSTV
PEYLKTADNRDRSFQLLEPLSTYNKNGNAVCSTATVVSTNHSAAASTPTTTATPHANP
ITHAHSLSNPNSTATMQHNPLAGKRGPKSGSTMGTPTVHNSTAAAPIAAPKKPRKPRQ
TKKAKAQAQAQAQAQAQVYAQQSTVQTPITASMAAALPNPTPSMINSVSPTNVMGTPL
TNMMSPMGNAYSMGAQNQGGQVSMSQFNGSGNGSNPNTNTNSNNTPLQSQLNLNNLTP
ANILNMSMNNDFQQQQQQQQQQQQPQPQYNMNMGMNNMNNGGKELDSLDLNNLELGGL
NMDFL"
gene complement(<450197..>452104)
/gene="PIB2"
/locus_tag="YGL023C"
/db_xref="GeneID:852861"
mRNA complement(<450197..>452104)
/gene="PIB2"
/locus_tag="YGL023C"
/product="Pib2p"
/transcript_id="NM_001180888.3"
/db_xref="GeneID:852861"
CDS complement(450197..452104)
/gene="PIB2"
/locus_tag="YGL023C"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:29698392]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0140785 amino acid
sensor activity [PMID:34535752|PMID:38127619]"
/experiment="EXISTENCE:direct assay:GO:1902485 L-cysteine
binding [PMID:38127619]"
/experiment="EXISTENCE:direct assay:GO:1904263 positive
regulation of TORC1 signaling [PMID:34535752]"
/experiment="EXISTENCE:genetic interaction:GO:1904263
positive regulation of TORC1 signaling [PMID:29698392]"
/experiment="EXISTENCE:mutant phenotype:GO:0032266
phosphatidylinositol-3-phosphate binding [PMID:29698392]"
/experiment="EXISTENCE:mutant phenotype:GO:1902485
L-cysteine binding [PMID:38127619]"
/experiment="EXISTENCE:mutant phenotype:GO:1904263
positive regulation of TORC1 signaling
[PMID:34535752|PMID:38127619]"
/note="Phosphatidylinositol 3-phosphate binding protein;
forms a distinct complex with TORC1, induced by glutamine
in a dose-dependent manner, resulting in TORC1 activation,
suggesting dual roles as both a glutamine sensor and a
direct TORC1 activator; functions as a cysteine sensor
involved in TORC1 activation; involved in leucine- and
glutamine-responsive TORC1 activation, independent of the
Gtr/Ego pathway; related to human PLEKHF1; contains a PI3P
binding FYVE domain; similar to Fab1p and Vps27p"
/codon_start=1
/product="Pib2p"
/protein_id="NP_011492.3"
/db_xref="GeneID:852861"
/db_xref="SGD:S000002991"
/translation="MTALHSVSKTPAIKEEEEDGDERDGRGVPLGPRNHDYRGRKGDE
ESGADTVTSPITFEKKKIAPRASTHSEQSILSSISLKSMVNQHRQQQLQQESSTGAGT
GFVDRKQQIQSPAMVSILRKNSAEENVRSSHSSKLGEGQIDGRKASASKEIGKTLPFT
DDQRSNPELDPTNSVVDVSRGKNTKSKTVFNELEDDADDDDEVRQKNLTTQALRKLSS
FKMNASSNLRLSKENKAKESSSSSTSSVSSSSTSKVENIVDKLTTTNSSSMSQLRFGN
TNVIIDSVNHAAKPPHQQMLRKPSLEFLPQPASSTNLNFNSNKHKSNVRQISNPKKPL
YIPAVLRKVSETNITNDDLLNATLSSYYKKASNLEHGFNPSKSQSASVQNANNLRIIS
SQSSVQSNTSSILESYKNKISSYLFPNSIPNSDRINLIPTISNRNSARVNPPTKDHWI
PDSKRNSCRYCHKPFTLWERKHHCRHCGDIFCQDHLRHWLYLDSQANFIMINELNNGG
INGGGTLCKICDDCLVEYENLSTTNHNANTNEDNINVEEGEDDDNDNRKKLRNYYKNR
QMNALFRPKKGGSSQEHATVDRDTTTPIQVKSNDEEADNENTGGEQEEGNDVLGSVIG
SVPANWNWSSF"
gene <452404..>454560
/gene="STT3"
/locus_tag="YGL022W"
/db_xref="GeneID:852862"
mRNA <452404..>454560
/gene="STT3"
/locus_tag="YGL022W"
/product="dolichyl-diphosphooligosaccharide--protein
glycosyltransferase subunit STT3"
/transcript_id="NM_001180887.1"
/db_xref="GeneID:852862"
CDS 452404..454560
/gene="STT3"
/locus_tag="YGL022W"
/EC_number="2.4.99.18"
/experiment="EXISTENCE:curator inference:GO:0004579
dolichyl-diphosphooligosaccharide-protein glycotransferase
activity [PMID:9405463]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0008250
oligosaccharyltransferase complex [PMID:10358084]"
/experiment="EXISTENCE:physical interaction:GO:0006487
protein N-linked glycosylation [PMID:9405463]"
/experiment="EXISTENCE:physical interaction:GO:0008250
oligosaccharyltransferase complex [PMID:9405463]"
/note="Catalytic subunit of the oligosaccharyltransferase
(OST) complex; the OST complex catalyzes asparagine-linked
glycosylation of newly synthesized proteins in the ER
lumen; involved in recognition of N-acetylglucosamine;
forms a subcomplex with Ost3p and Ost4p"
/codon_start=1
/product="dolichyl-diphosphooligosaccharide--protein
glycosyltransferase subunit STT3"
/protein_id="NP_011493.1"
/db_xref="GeneID:852862"
/db_xref="SGD:S000002990"
/translation="MGSDRSCVLSVFQTILKLVIFVAIFGAAISSRLFAVIKFESIIH
EFDPWFNYRATKYLVNNSFYKFLNWFDDRTWYPLGRVTGGTLYPGLMTTSAFIWHALR
NWLGLPIDIRNVCVLFAPLFSGVTAWATYEFTKEIKDASAGLLAAGFIAIVPGYISRS
VAGSYDNEAIAITLLMVTFMFWIKAQKTGSIMHATCAALFYFYMVSAWGGYVFITNLI
PLHVFLLILMGRYSSKLYSAYTTWYAIGTVASMQIPFVGFLPIRSNDHMAALGVFGLI
QIVAFGDFVKGQISTAKFKVIMMVSLFLILVLGVVGLSALTYMGLIAPWTGRFYSLWD
TNYAKIHIPIIASVSEHQPVSWPAFFFDTHFLIWLFPAGVFLLFLDLKDEHVFVIAYS
VLCSYFAGVMVRLMLTLTPVICVSAAVALSKIFDIYLDFKTSDRKYAIKPAALLAKLI
VSGSFIFYLYLFVFHSTWVTRTAYSSPSVVLPSQTPDGKLALIDDFREAYYWLRMNSD
EDSKVAAWWDYGYQIGGMADRTTLVDNNTWNNTHIAIVGKAMASPEEKSYEILKEHDV
DYVLVIFGGLIGFGGDDINKFLWMIRISEGIWPEEIKERDFYTAEGEYRVDARASETM
RNSLLYKMSYKDFPQLFNGGQATDRVRQQMITPLDVPPLDYFDEVFTSENWMVRIYQL
KKDDAQGRTLRDVGELTRSSTKTRRSIKRPELGLRV"
gene <454785..>457067
/gene="ALK1"
/locus_tag="YGL021W"
/db_xref="GeneID:852863"
mRNA <454785..>457067
/gene="ALK1"
/locus_tag="YGL021W"
/product="protein kinase ALK1"
/transcript_id="NM_001180886.1"
/db_xref="GeneID:852863"
CDS 454785..457067
/gene="ALK1"
/locus_tag="YGL021W"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16855400]"
/experiment="EXISTENCE:mutant phenotype:GO:0044879 mitotic
morphogenesis checkpoint signaling [PMID:33585466]"
/note="Atypical protein kinase; along with paralog, ALK2,
required for proper spindle positioning and nuclear
segregation following mitotic arrest, proper organization
of formins and polarisome components in mitosis, and
survival after spindle assembly checkpoint delay;
regulates the morphogenesis checkpoint in response to
polarity insults through inactivation of Mih1p;
phosphorylated in response to DNA damage; similar to
mammalian haspins"
/codon_start=1
/product="protein kinase ALK1"
/protein_id="NP_011494.1"
/db_xref="GeneID:852863"
/db_xref="SGD:S000002989"
/translation="MLLHFDIVIQLLSSHTLKSHQVEPPMDFETSFEEFVEDKRFIAL
EVSDNDDDCDTDLTADTADELESSAILKMRESDASLNVTTGNNTSRKTTSNSKKRWSL
LSNHSAVSSSKSKKRWSVLSSSFTSESHKDRESRNVLQQKRKSLQSYSSLDTVASNSS
ISASSSLKRSSTGLSLRQLFTKIGINDDISQPGIGIPQGKENLPPTMGKKNSSIASTS
SENRLRTPLKPLVNHSKRPTSQPQQQQPLYNASLSSRRSSISSTVSSSSSSKWRFWKR
NKNQTPALLQPDHHSLKTFPAVNRRDSMTPVEPRNMVKHKTSFSDFHKTIFSSNTYSE
SSDTISSMEITLKNKASSSSLSLNVLKKRNSQSSLKHKSSHASLQKFKRNKGKSSMIA
PSTATNSSNDDSCSYSSKNSTLSHRISLPVPDQVSRDKIQNKLRYSTSLLSLNSKSSL
PMNKNDHDETLLRQILLNCDIKRILNPAKGDVLPLINDVNHLSSIQLTSNVWQIGEVI
CKKVSLGTIDDITWDRKFLSLQELEKLKIMQQKFDGIPQLLKSFVVKEANGGLYLYLL
FKDHGTPISLISLKNWKQILKIFWSCAGIIHGLEKNLKFEHRNLTLDNILIDGNGNIT
IIDFKCSRLQTPQDDVLYLRLDHPLFFLNGKDKSKINEYQYQFEFEIYQSMRILLNMD
ASAFEPMTNLYWLYYLSRVLLKFGDRKLGKNDANRDKMARVINHLEMNLAVHKRGGQL
FKRLETEDIKNTGDLLKLYK"
gene complement(<457163..>457870)
/gene="GET1"
/locus_tag="YGL020C"
/gene_synonym="MDM39"
/db_xref="GeneID:852864"
mRNA complement(<457163..>457870)
/gene="GET1"
/locus_tag="YGL020C"
/gene_synonym="MDM39"
/product="GET complex subunit GET1"
/transcript_id="NM_001180885.1"
/db_xref="GeneID:852864"
CDS complement(457163..457870)
/gene="GET1"
/locus_tag="YGL020C"
/gene_synonym="MDM39"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:36640319|PMID:25043001]"
/experiment="EXISTENCE:direct assay:GO:0006890 retrograde
vesicle-mediated transport, Golgi to endoplasmic reticulum
[PMID:16269340]"
/experiment="EXISTENCE:direct assay:GO:0008320 protein
transmembrane transporter activity [PMID:36640319]"
/experiment="EXISTENCE:direct assay:GO:0097051
establishment of protein localization to endoplasmic
reticulum membrane [PMID:36640319]"
/experiment="EXISTENCE:genetic interaction:GO:0006890
retrograde vesicle-mediated transport, Golgi to
endoplasmic reticulum [PMID:16269340]"
/experiment="EXISTENCE:genetic interaction:GO:0043495
protein-membrane adaptor activity [PMID:18724936]"
/experiment="EXISTENCE:genetic interaction:GO:0071816
tail-anchored membrane protein insertion into ER membrane
[PMID:18724936]"
/experiment="EXISTENCE:mutant phenotype:GO:0000423
mitophagy [PMID:29673596]"
/experiment="EXISTENCE:mutant phenotype:GO:0006890
retrograde vesicle-mediated transport, Golgi to
endoplasmic reticulum [PMID:16269340]"
/experiment="EXISTENCE:mutant phenotype:GO:0032977
membrane insertase activity [PMID:25043001]"
/experiment="EXISTENCE:physical interaction:GO:0043529 GET
complex [PMID:16269340]"
/note="Subunit of the GET complex; involved in insertion
of tail-anchored proteins into the ER membrane; required
for the retrieval of HDEL proteins from the Golgi to the
ER in an ERD2 dependent fashion and for normal
mitochondrial morphology and inheritance; Get1p and Get2p
form an aqueous channel for protein translocation and
insertion into the membrane"
/codon_start=1
/product="GET complex subunit GET1"
/protein_id="NP_011495.1"
/db_xref="GeneID:852864"
/db_xref="SGD:S000002988"
/translation="MHWAAAVAIFFIVVTKFLQYTNKYHEKWISKFAPGNELSKKYLA
KVKERHELKEFNNSISAQDNYAKWTKNNRKLDSLDKEINNLKDEIQSENKAFQAHLHK
LRLLALTVPFFVFKIMYGKTPVYKLSSSTSTLFPTFVSGVWSQGWLYVLLHPLRTISQ
KWHIMEGKFGASKFDDMALQSVSLGIWVWALMNVINGVEFIVKQLFLTPKMEAPASVE
TQEEKALDAVDDAIILD"
gene <458156..>458992
/gene="CKB1"
/locus_tag="YGL019W"
/db_xref="GeneID:852865"
mRNA <458156..>458992
/gene="CKB1"
/locus_tag="YGL019W"
/product="casein kinase 2 regulatory subunit CKB1"
/transcript_id="NM_001180884.3"
/db_xref="GeneID:852865"
CDS 458156..458992
/gene="CKB1"
/locus_tag="YGL019W"
/experiment="EXISTENCE:direct assay:GO:0005956 protein
kinase CK2 complex [PMID:8135547]"
/experiment="EXISTENCE:direct assay:GO:0006356 regulation
of transcription by RNA polymerase I [PMID:11551505]"
/experiment="EXISTENCE:direct assay:GO:0006359 regulation
of transcription by RNA polymerase III [PMID:11551505]"
/experiment="EXISTENCE:direct assay:GO:0006974 DNA damage
response [PMID:11551505]"
/experiment="EXISTENCE:direct assay:GO:0019887 protein
kinase regulator activity [PMID:8135547]"
/experiment="EXISTENCE:direct assay:GO:0030291 protein
serine/threonine kinase inhibitor activity
[PMID:18265947]"
/experiment="EXISTENCE:direct assay:GO:0034456 UTP-C
complex [PMID:17515605]"
/experiment="EXISTENCE:mutant phenotype:GO:0019887 protein
kinase regulator activity [PMID:11827175]"
/note="Beta regulatory subunit of casein kinase 2 (CK2); a
Ser/Thr protein kinase with roles in cell growth and
proliferation; CK2, comprised of CKA1, CKA2, CKB1 and
CKB2, has many substrates including transcription factors
and all RNA polymerases"
/codon_start=1
/product="casein kinase 2 regulatory subunit CKB1"
/protein_id="NP_011496.3"
/db_xref="GeneID:852865"
/db_xref="SGD:S000002987"
/translation="MSQEFVEDYSRTGSSDDEDSGAYDEWIPSFCSRFGHEYFCQVPT
EFIEDDFNMTSLSQEVPHYRKALDLILDLEAMSDEEEDEDDVVEEDEVDQEMQSNDGH
DEGKRRNKSPVVNKSIIEHAAEQLYGLIHARFILTKPGLQAMAEKFDHKEFGTCPRYY
CNGMQLLPCGLSDTVGKHTVRLYCPSCQDLYLPQSSRFLCLEGAFWGTSFPGVFLKHF
KELEEYVERKSKESYELKVFGFRINDEAVSGPRMKWLRQYPSTEEDWEEFAKCEFETP
AV"
gene complement(<459110..>459664)
/gene="JAC1"
/locus_tag="YGL018C"
/db_xref="GeneID:852866"
mRNA complement(<459110..>459664)
/gene="JAC1"
/locus_tag="YGL018C"
/product="J-type chaperone JAC1"
/transcript_id="NM_001180883.1"
/db_xref="GeneID:852866"
CDS complement(459110..459664)
/gene="JAC1"
/locus_tag="YGL018C"
/experiment="EXISTENCE:direct assay:GO:0001671 ATPase
activator activity [PMID:12756240]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:11278728|PMID:16823961|PMID:14576278|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:11273703|PMID:11171977]"
/experiment="EXISTENCE:mutant phenotype:GO:0005739
mitochondrion [PMID:11273703]"
/experiment="EXISTENCE:mutant phenotype:GO:0006879
intracellular iron ion homeostasis [PMID:11278728]"
/experiment="EXISTENCE:mutant phenotype:GO:0009060 aerobic
respiration [PMID:9813017]"
/experiment="EXISTENCE:mutant phenotype:GO:0016226
iron-sulfur cluster assembly [PMID:11171977]"
/experiment="EXISTENCE:mutant phenotype:GO:0044571
[2Fe-2S] cluster assembly [PMID:11273703]"
/experiment="EXISTENCE:mutant phenotype:GO:0044572
[4Fe-4S] cluster assembly [PMID:11278728]"
/experiment="EXISTENCE:mutant phenotype:GO:0051087
protein-folding chaperone binding [PMID:11278728]"
/note="Specialized J-protein that functions in Fe-S
cluster biogenesis; functions with Hsp70 in Fe-S cluster
biogenesis in mitochondria; involved in iron metabolism;
contains a J domain typical to J-type chaperones;
localizes to the mitochondrial matrix"
/codon_start=1
/product="J-type chaperone JAC1"
/protein_id="NP_011497.1"
/db_xref="GeneID:852866"
/db_xref="SGD:S000002986"
/translation="MLKYLVQRRFTSTFYELFPKTFPKKLPIWTIDQSRLRKEYRQLQ
AQHHPDMAQQGSEQSSTLNQAYHTLKDPLRRSQYMLKLLRNIDLTQEQTSNEVTTSDP
QLLLKVLDIHDELSQMDDEAGVKLLEKQNKERIQDIEAQLGQCYNDKDYAAAVKLTVE
LKYWYNLAKAFKDWAPGKQLEMNH"
gene <459854..>461365
/gene="ATE1"
/locus_tag="YGL017W"
/db_xref="GeneID:852867"
mRNA <459854..>461365
/gene="ATE1"
/locus_tag="YGL017W"
/product="arginyltransferase"
/transcript_id="NM_001180882.1"
/db_xref="GeneID:852867"
CDS 459854..461365
/gene="ATE1"
/locus_tag="YGL017W"
/EC_number="2.3.2.8"
/experiment="EXISTENCE:direct assay:GO:0004057
arginyl-tRNA--protein transferase activity [PMID:2185248]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0006915
apoptotic process [PMID:40818972]"
/experiment="EXISTENCE:mutant phenotype:GO:0004057
arginyl-tRNA--protein transferase activity [PMID:2185248]"
/experiment="EXISTENCE:mutant phenotype:GO:0006915
apoptotic process [PMID:40818972]"
/experiment="EXISTENCE:mutant phenotype:GO:0071596
ubiquitin-dependent protein catabolic process via the
N-end rule pathway [PMID:2185248]"
/note="Arginyl-tRNA-protein transferase; catalyzes
post-translational conjugation of arginine, a
destabilizing residue, to the N-termini of acceptor
proteins and increasing the degradation of substrates with
secondary destabilizing residues Asp and Glu via the N-end
rule pathway; mitochondrial translocation in response to
oxidative stress induces apoptosis; may play a role in
regulating stress response"
/codon_start=1
/product="arginyltransferase"
/protein_id="NP_011498.1"
/db_xref="GeneID:852867"
/db_xref="SGD:S000002985"
/translation="MSDRFVIWAPSMHNEPAAKCGYCHGNKGGNMDQLFALDSWAHRY
MNKMDVVKIENCTIGSFVEHMDVATYDRMCNMGFRRSGKFLYKVDPLRNCCRLYTIRT
APQELNMTKELKKCISRFATRITSEDYCPAAVASSDFVGKIVNAEMNSKTFYTRFEPA
LYSEEKYHLFVKYQEKVHQDYNNSPKSFKRFLCDTPFGPEAVLGTQESWEQLNNWQRM
KPGEKLKHMGPVHECYYYEGKLIAITVSDILPSGISSVYFIWDPDYSKWSLGKLSALR
DLAIIQRTNLQYYYLGYYIEDCPKMNYKANYGAEVLDVCHSKYIPLKPIQDMISRGKL
FVIGEEETKVTKELYLVDSETGRGEGFPTDNVVKYKNIAEEIYGVGGCAFKSANESAL
ELKELYGIPYEEEDLDTIYHLKEHNGHAPNGIPNVVPGLLPLWELLDIMQSGKITDLE
GRLFLFEIETEGIRPLINFYSEPPNVKKRICDVIRLFGFETCMKAVILYSEQM"
gene <461666..>464911
/gene="KAP122"
/locus_tag="YGL016W"
/gene_synonym="PDR6"
/db_xref="GeneID:852868"
mRNA <461666..>464911
/gene="KAP122"
/locus_tag="YGL016W"
/gene_synonym="PDR6"
/product="Kap122p"
/transcript_id="NM_001180881.1"
/db_xref="GeneID:852868"
CDS 461666..464911
/gene="KAP122"
/locus_tag="YGL016W"
/gene_synonym="PDR6"
/experiment="EXISTENCE:direct assay:GO:0005049 nuclear
export signal receptor activity [PMID:31023724]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10684247]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:10684247]"
/experiment="EXISTENCE:direct assay:GO:0006606 protein
import into nucleus [PMID:10525531]"
/experiment="EXISTENCE:direct assay:GO:0051168 nuclear
export [PMID:31023724]"
/experiment="EXISTENCE:mutant phenotype:GO:0006606 protein
import into nucleus [PMID:10525531]"
/experiment="EXISTENCE:mutant phenotype:GO:0008361
regulation of cell size [PMID:12089449]"
/experiment="EXISTENCE:mutant phenotype:GO:0061608 nuclear
import signal receptor activity [PMID:10525531]"
/experiment="EXISTENCE:physical interaction:GO:0061608
nuclear import signal receptor activity [PMID:10525531]"
/note="Karyopherin beta; bidirectional nuclear transport
receptor (biportin) that shuttles distinct sets of cargoes
in opposite directions; responsible for import of
Toa1p-Toa2p complex into nucleus; depletes eIF5A and eEF2
from cell nuclei; binds to nucleoporins Nup1p and Nup2p;
may play a role in regulation of pleiotropic drug
resistance"
/codon_start=1
/product="Kap122p"
/protein_id="NP_011499.1"
/db_xref="GeneID:852868"
/db_xref="SGD:S000002984"
/translation="MSSIHEVVALIEELYSPHPKHDVNQIQQSLQSIQKSEQGFHLAN
ELLSDDKYSANVKYFGALTLTVQLNTRGENDYETLWNVFRSNLLYLTKFSTLYVSNPN
MYGQSLIIIKKLMSNLSLIFTKINDPQLNNAGNENMIKQWNNPINTFIQLMSVQNQNI
NADQLLLDSINCSLTYEQLSQFVSLSQKHNELALTFTEVIVEDLTKFQTKRHSMSQIH
EVVHEHLYISTMALINLNLTAQAVFNPTVFDCITAWINYISLTRSVSSSGRMDLSEIF
QNLIDLMYQSTEGSDGYENAEKILTIFGNVFANDPLLMSYDLRQQIECIFLGVVRPDS
GITDISNKNSWMLQYMNYLVTNDFFSELKELAICIVDFLQINTLSVCNKLFTNIQAAD
NGQVQDEYIQEYIKVLLQMTNFPLTPVLQEFFSVRMVDFWLDLSDAYTNLASETLRPN
SIELSTQIFQQLINIYLPKISLSVKQRIIEEEGESTSVNEFEDFRNAVSDLAQSLWSI
LGNDNLTNVLIDGMGQMPAASDETLIIKDTDVLFRIETMCFVLNTILVDMTLSESPWI
KNIVDANKFFNQNVISVFQTGFQTSASTKVSQILKLDFVRTSTTLIGTLAGYFKQEPF
QLNPYVEALFQGLHTCTNFTSKNEQEKISNDKLEVMVIKTVSTLCETCREELTPYLMH
FISFLNTVIMPDSNVSHFTRTKLVRSIGYVVQCQVSNGPEEQAKYILQLTNLLSGSIE
HCLASSVQLQEQQDYINCLLYCISELATSLIQPTEIIENDALLQRLSEFQSFWSSDPL
QIRSKIMCTIDKVLDNSIYCKNSAFVEIGCLIVGKGLNLPDGEPYFLKYNMSEVMNFV
LRHVPNCELATCLPYFVYLLEKLISEFRKELTPQEFDFMFEKILLVYYDAYIINDPDL
LQMTIGFVNNVLDVKPGLAIGSKHWTSFILPQFLKLIPSREKFTIVAVAKFWTKLINN
KKYNQEELTTVRQQVSSIGGDLVYQIMYGLFHTQRSDLNSYTDLLRALVAKFPIEARE
WLVAVLPQICNNPAGHEKFINKLLITRGSRAAGNVILQWWLDCTTLPNYQG"
gene complement(<465043..>465435)
/gene="BIL2"
/locus_tag="YGL015C"
/db_xref="GeneID:852869"
mRNA complement(<465043..>465435)
/gene="BIL2"
/locus_tag="YGL015C"
/product="Bil2p"
/transcript_id="NM_001180880.3"
/db_xref="GeneID:852869"
CDS complement(465043..465435)
/gene="BIL2"
/locus_tag="YGL015C"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:33634134|PMID:38656798]"
/experiment="EXISTENCE:direct assay:GO:0030427 site of
polarized growth [PMID:33634134]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:38656798]"
/experiment="EXISTENCE:direct assay:GO:0090339 negative
regulation of formin-nucleated actin cable assembly
[PMID:33634134]"
/experiment="EXISTENCE:direct assay:GO:0099503 secretory
vesicle [PMID:33634134]"
/experiment="EXISTENCE:mutant phenotype:GO:1903260 protein
localization to mating projection tip [PMID:38656798]"
/note="Formin inhibitor; inhibits Bnr1p-mediated actin
filament nucleation but not elongation during vegetative
growth; performs distinct but overlapping roles with Hof1p
to control actin cable assembly and polarized secretion;
spatially focuses Bni1p at mating projection tips, and
with Bud6p organizes Bni1p into clusters that nucleate
actin assembly during mating projection formation; bnr1
bil2 double mutants are inviable; localizes to the
cytosol, secretory vesicles and sites of polarized growth"
/codon_start=1
/product="Bil2p"
/protein_id="NP_011500.3"
/db_xref="GeneID:852869"
/db_xref="SGD:S000002983"
/translation="MEDTIRPLNYADIETSGPINLLETTNNLKSSLKKFSQKAKGSHI
SRERIHHFRKWKNKTESLSENHLKPPPDVDSLCFSNCFQPDALSGNVFLPPRSSNMYW
NEKQLQLEMEILKFLSLNTSNECCTSDD"
gene <466141..>468807
/gene="PUF4"
/locus_tag="YGL014W"
/gene_synonym="YGL023"
/db_xref="GeneID:852870"
mRNA <466141..>468807
/gene="PUF4"
/locus_tag="YGL014W"
/gene_synonym="YGL023"
/product="Puf4p"
/transcript_id="NM_001180879.1"
/db_xref="GeneID:852870"
CDS 466141..468807
/gene="PUF4"
/locus_tag="YGL014W"
/gene_synonym="YGL023"
/experiment="EXISTENCE:direct assay:GO:0000288
nuclear-transcribed mRNA catabolic process,
deadenylation-dependent decay [PMID:17389596]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:15024427|PMID:18327269|PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0017148 negative
regulation of translation [PMID:20427513]"
/experiment="EXISTENCE:genetic interaction:GO:0000288
nuclear-transcribed mRNA catabolic process,
deadenylation-dependent decay [PMID:11101532]"
/experiment="EXISTENCE:mutant phenotype:GO:0008104
intracellular protein localization [PMID:9150138]"
/note="Member of the PUF protein family; PUF family is
defined by the presence of Pumilio homology domains that
confer RNA binding activity; preferentially binds mRNAs
encoding nucleolar ribosomal RNA-processing factors"
/codon_start=1
/product="Puf4p"
/protein_id="NP_011501.1"
/db_xref="GeneID:852870"
/db_xref="SGD:S000002982"
/translation="MSTKGLKEEIDDVPSVDPVVSETVNSALEQLQLDDPEENATSNA
FANKVSQDSQFANGPPSQMFPHPQMMGGMGFMPYSQMMQVPHNPCPFFPPPDFNDPTA
PLSSSPLNAGGPPMLFKNDSLPFQMLSSGAAVATQGGQNLNPLINDNSMKVLPIASAD
PLWTHSNVPGSASVAIEETTATLQESLPSKGRESNNKASSFRRQTFHALSPTDLINAA
NNVTLSKDFQSDMQNFSKAKKPSVGANNTAKTRTQSISFDNTPSSTSFIPPTNSVSEK
LSDFKIETSKEDLINKTAPAKKESPTTYGAAYPYGGPLLQPNPIMPGHPHNISSPIYG
IRSPFPNSYEMGAQFQPFSPILNPTSHSLNANSPIPLTQSPIHLAPVLNPSSNSVAFS
DMKNDGGKPTTDNDKAGPNVRMDLINPNLGPSMQPFHILPPQQNTPPPPWLYSTPPPF
NAMVPPHLLAQNHMPLMNSANNKHHGRNNNSMSSHNDNDNIGNSNYNNKDTGRSNVGK
MKNMKNSYHGYYNNNNNNNNNNNNNNNSNATNSNSAEKQRKIEESSRFADAVLDQYIG
SIHSLCKDQHGCRFLQKQLDILGSKAADAIFEETKDYTVELMTDSFGNYLIQKLLEEV
TTEQRIVLTKISSPHFVEISLNPHGTRALQKLIECIKTDEEAQIVVDSLRPYTVQLSK
DLNGNHVIQKCLQRLKPENFQFIFDAISDSCIDIATHRHGCCVLQRCLDHGTTEQCDN
LCDKLLALVDKLTLDPFGNYVVQYIITKEAEKNKYDYTHKIVHLLKPRAIELSIHKFG
SNVIEKILKTAIVSEPMILEILNNGGETGIQSLLNDSYGNYVLQTALDISHKQNDYLY
KRLSEIVAPLLVGPIRNTPHGKRIIGMLHLDS"
gene complement(<469092..>472298)
/gene="PDR1"
/locus_tag="YGL013C"
/gene_synonym="AMY1; ANT1; BOR2; CYH3; NRA2; SMR2; TIL1;
TPE1; TPE3"
/db_xref="GeneID:852871"
mRNA complement(<469092..>472298)
/gene="PDR1"
/locus_tag="YGL013C"
/gene_synonym="AMY1; ANT1; BOR2; CYH3; NRA2; SMR2; TIL1;
TPE1; TPE3"
/product="drug-responsive transcription factor PDR1"
/transcript_id="NM_001180878.1"
/db_xref="GeneID:852871"
CDS complement(469092..472298)
/gene="PDR1"
/locus_tag="YGL013C"
/gene_synonym="AMY1; ANT1; BOR2; CYH3; NRA2; SMR2; TIL1;
TPE1; TPE3"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11136452|PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:genetic interaction:GO:2001040
positive regulation of cellular response to drug
[PMID:8078477]"
/experiment="EXISTENCE:mutant phenotype:GO:0001228
DNA-binding transcription activator activity, RNA
polymerase II-specific [PMID:8798494|PMID:9428726]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:9428726|PMID:8798494]"
/experiment="EXISTENCE:mutant phenotype:GO:0071466
cellular response to xenobiotic stimulus
[PMID:9428726|PMID:8798494]"
/experiment="EXISTENCE:mutant phenotype:GO:2001040
positive regulation of cellular response to drug
[PMID:15713640|PMID:9767597]"
/note="Transcription factor that regulates the pleiotropic
drug response; zinc cluster protein that is a master
regulator involved in recruiting other zinc cluster
proteins to pleiotropic drug response elements (PDREs) to
fine tune the regulation of multidrug resistance genes;
relocalizes to the cytosol in response to hypoxia; PDR1
has a paralog, PDR3, that arose from the whole genome
duplication"
/codon_start=1
/product="drug-responsive transcription factor PDR1"
/protein_id="NP_011502.1"
/db_xref="GeneID:852871"
/db_xref="SGD:S000002981"
/translation="MRGLTPKNGVHIETGPDTESSADSSNFSTGFSGKIRKPRSKVSK
ACDNCRKRKIKCNGKFPCASCEIYSCECTFSTRQGGARIKNLHKTSLEGTTVQVKEET
DSSSTSFSNPQRCTDGPCAVEQPTKFFENFKLGGRSSGDNSGSDGKNDDDVNRNGFYE
DDSESQATLTSLQTTLKNLKEMAHLGTHVTSAIESIELQISDLLKRWEPKVRTKELAT
TKFYPNKSIETQLMKNKYCDVVHLTRYAAWSNNKKDQDTSSQPLIDEIFGLYSPFQFL
SLQGIGKCFQNYRSKSKCEIFPRTAKETIYIMLRFFDVCFHHINQGCVSIANPLENYL
QKMNLLPSTPSSISSAGSPNTAHTKSHVALVINHLPQPFVRNITGISNSELLSEMNND
ISMFGILLKMLDMHKNSYQNFLMEITSNPSVAKNTQSIDVLQEFIHYCQAGEALIALC
YSYYNSTLYNYVDFTCDITHLEQLLYFLDLLFWLSEIYGFEKVLNVAVHFVSRVGLSR
WEFYVGLDENFAERRRNLWWKAFYFEKTLASKLGYPSNIDDSKINCLLPKNFRDVGFL
DNRDFIENVHLVRRSEAFDNMCISDLKYYGELAVLQIVSHFSSSVLFNEKFTSIRNTS
KPSVVREKLLFEVLEIFNETEMKYDAIKEQTGKLFDIAFSKDSTELKVSREDKIMASK
FVLFYEHHFCRMVNESDNIVARLCVHRRPSILIENLKIYLHKIYKSWTDMNKILLDFD
NDYSVYRSFAHYSISCIILVSQAFSVAEFIKVNDVVNMIRVFKRFLDIKIFSENETNE
HVFNSQSFKDYTRAFSFLTIVTRIMLLAYGESSSTNLDVISKYIDENAPDLKGIIELV
LDTNSCAYRFLLEPVQKSGFHLTVSQMLKNRKFQEPLMSNEDNKQMKHNSGKNLNPDL
PSLKTGTSCLLNGIESPQLPFNGRSAPSPVRNNSLPEFAQLPSFRSLSVSDMINPDYA
QPTNGQNNTQVQSNKPINAQQQIPTSVQVPFMNTNEINNNNNNNNNNKNNINNINNNN
SNNFSATSFNLGTLDEFVNNGDLEDLYSILWSDVYPDS"
gene <472855..>474276
/gene="ERG4"
/locus_tag="YGL012W"
/db_xref="GeneID:852872"
mRNA <472855..>474276
/gene="ERG4"
/locus_tag="YGL012W"
/product="delta(24(24(1)))-sterol reductase"
/transcript_id="NM_001180877.1"
/db_xref="GeneID:852872"
CDS 472855..474276
/gene="ERG4"
/locus_tag="YGL012W"
/EC_number="1.3.1.71"
/experiment="EXISTENCE:direct assay:GO:0000246
Delta24(24-1) sterol reductase activity [PMID:14922]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762|PMID:10722850]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:10722850]"
/experiment="EXISTENCE:direct assay:GO:0050614
Delta24-sterol reductase activity [PMID:10722850]"
/experiment="EXISTENCE:mutant phenotype:GO:0006696
ergosterol biosynthetic process [PMID:8125337]"
/note="C-24(28) sterol reductase; catalyzes the final step
in ergosterol biosynthesis; mutants are viable, but lack
ergosterol"
/codon_start=1
/product="delta(24(24(1)))-sterol reductase"
/protein_id="NP_011503.1"
/db_xref="GeneID:852872"
/db_xref="SGD:S000002980"
/translation="MAKDNSEKLQVQGEEKKSKQPVNFLPQGKWLKPNEIEYEFGGTT
GVIGMLIGFPLLMYYMWICAEFYHGKVALPKAGESWMHFIKHLYQLVLENGIPEKYDW
TIFLTFWVFQIIFYYTLPGIWTKGQPLSHLKGKQLPYFCNAMWTLYVTTTLVLVLHFT
NLFRLYVIIDRFGRIMTCAIISGFAFSIILYLWTLFISHDYHRMTGNHLYDFFMGAPL
NPRWGILDLKMFFEVRLPWFTLYFITLGACLKQWETYGYVTPQLGVVMLAHWLYANAC
AKGEELIVPTWDMAYEKFGFMLIFWNIAGVPYTYCHCTLYLYYHDPSEYHWSTLYNVS
LYVVLLCAYYFFDTANAQKNAFRKQMSGDKTGRKTFPFLPYQILKNPKYMVTSNGSYL
LIDGWYTLARKIHYTADWTQSLVWALSCGFNSVFPWFFPVFFLVVLIHRAFRDQAKCK
RKYGKDWDEYCKHCPYVFIPYVF"
gene complement(<474489..>475247)
/gene="SCL1"
/locus_tag="YGL011C"
/gene_synonym="PRC2"
/db_xref="GeneID:852873"
mRNA complement(<474489..>475247)
/gene="SCL1"
/locus_tag="YGL011C"
/gene_synonym="PRC2"
/product="proteasome core particle subunit alpha 1"
/transcript_id="NM_001180876.3"
/db_xref="GeneID:852873"
CDS complement(474489..475247)
/gene="SCL1"
/locus_tag="YGL011C"
/gene_synonym="PRC2"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0010499 proteasomal
ubiquitin-independent protein catabolic process
[PMID:19162040]"
/experiment="EXISTENCE:direct assay:GO:0019773 proteasome
core complex, alpha-subunit complex [PMID:9087403]"
/experiment="EXISTENCE:direct assay:GO:0043161
proteasome-mediated ubiquitin-dependent protein catabolic
process [PMID:11545745|PMID:19029916]"
/note="Alpha 1 subunit of the 20S proteasome; involved in
the degradation of ubiquitinated substrates; 20S
proteasome is the core complex of the 26S proteasome;
essential for growth; detected in the mitochondria"
/codon_start=1
/product="proteasome core particle subunit alpha 1"
/protein_id="NP_011504.3"
/db_xref="GeneID:852873"
/db_xref="SGD:S000002979"
/translation="MSGAAAASAAGYDRHITIFSPEGRLYQVEYAFKATNQTNINSLA
VRGKDCTVVISQKKVPDKLLDPTTVSYIFCISRTIGMVVNGPIPDARNAALRAKAEAA
EFRYKYGYDMPCDVLAKRMANLSQIYTQRAYMRPLGVILTFVSVDEELGPSIYKTDPA
GYYVGYKATATGPKQQEITTNLENHFKKSKIDHINEESWEKVVEFAITHMIDALGTEF
SKNDLEVGVATKDKFFTLSAENIEERLVAIAEQD"
gene <475545..>476069
/gene="MPO1"
/locus_tag="YGL010W"
/db_xref="GeneID:852874"
mRNA <475545..>476069
/gene="MPO1"
/locus_tag="YGL010W"
/product="2-hydroxy-palmitic acid dioxygenase MPO1"
/transcript_id="NM_001180875.1"
/db_xref="GeneID:852874"
CDS 475545..476069
/gene="MPO1"
/locus_tag="YGL010W"
/EC_number="1.14.18.12"
/experiment="EXISTENCE:direct assay:GO:0001561 fatty acid
alpha-oxidation [PMID:30530523]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0102672 fatty acid
alpha-dioxygenase activity [PMID:30530523]"
/experiment="EXISTENCE:mutant phenotype:GO:0046521
sphingoid catabolic process [PMID:25345524]"
/note="Dioxygenase that catalyzes alpha-oxygenation of
2-hydroxy fatty acids; involved in metabolism of
phytosphingosine; not an essential gene"
/codon_start=1
/product="2-hydroxy-palmitic acid dioxygenase MPO1"
/protein_id="NP_011505.1"
/db_xref="GeneID:852874"
/db_xref="SGD:S000002978"
/translation="MGEGLLDLRSQLGFYKFYHHNPKNVLIHSIFVPTILFSGSCMLH
RVKIYQSISLTAVLSVLFSIFYCLLYLPTGLLAGVLLLLLNLALIDHRVDLTFKQELG
LFTIGWIFQFVGHGVFEKRRPALIDNLVQSLVLAPYFIMFEFLFKLGFMPRLKATLEH
DLEIKQRNLRMQRQ"
gene complement(<476313..>478652)
/gene="LEU1"
/locus_tag="YGL009C"
/db_xref="GeneID:852875"
mRNA complement(<476313..>478652)
/gene="LEU1"
/locus_tag="YGL009C"
/product="3-isopropylmalate dehydratase LEU1"
/transcript_id="NM_001180874.1"
/db_xref="GeneID:852875"
CDS complement(476313..478652)
/gene="LEU1"
/locus_tag="YGL009C"
/EC_number="4.2.1.33"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:4355481]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405]"
/experiment="EXISTENCE:mutant phenotype:GO:0003861
3-isopropylmalate dehydratase activity [PMID:5724969]"
/experiment="EXISTENCE:mutant phenotype:GO:0009098
L-leucine biosynthetic process
[PMID:5724969|PMID:6323436]"
/note="Isopropylmalate isomerase; catalyzes the second
step in the leucine biosynthesis pathway"
/codon_start=1
/product="3-isopropylmalate dehydratase LEU1"
/protein_id="NP_011506.1"
/db_xref="GeneID:852875"
/db_xref="SGD:S000002977"
/translation="MVYTPSKGPRTLYDKVFDAHVVHQDENGSFLLYIDRHLVHEVTS
PQAFEGLENAGRKVRRVDCTLATVDHNIPTESRKNFKSLDTFIKQTDSRLQVKTLENN
VKQFGVPYFGMSDARQGIVHTIGPEEGFTLPGTTVVCGDSHTSTHGAFGSLAFGIGTS
EVEHVLATQTIIQAKSKNMRITVNGKLSPGITSKDLILYIIGLIGTAGGTGCVIEFAG
EAIEALSMEARMSMCNMAIEAGARAGMIKPDETTFQYTKGRPLAPKGAEWEKAVAYWK
TLKTDEGAKFDHEINIEAVDVIPTITWGTSPQDALPITGSVPDPKNVTDPIKKSGMER
ALAYMGLEPNTPLKSIKVDKVFIGSCTNGRIEDLRSAAAVVRGQKLASNIKLAMVVPG
SGLVKKQAEAEGLDKIFQEAGFEWREAGCSICLGMNPDILDAYERCASTSNRNFEGRQ
GALSRTHLMSPAMAAAAGIAGHFVDIREFEYKDQDQSSPKVEVTSEDEKELESAAYDH
AEPVQPEDAPQDIANDELKDIPVKSDDTPAKPSSSGMKPFLTLEGISAPLDKANVDTD
AIIPKQFLKTIKRTGLKKGLFYEWRFRKDDQGKDQETDFVLNVEPWREAEILVVTGDN
FGCGSSREHAPWALKDFGIKSIIAPSYGDIFYNNSFKNGLLPIRLDQQIIIDKLIPIA
NKGGKLCVDLPNQKILDSDGNVLVDHFEIEPFRKHCLVNGLDDIGITLQKEEYISRYE
ALRREKYSFLEGGSKLLKFDNVPKRKAVTTTFDKVHQDW"
gene complement(<479910..>482666)
/gene="PMA1"
/locus_tag="YGL008C"
/gene_synonym="KTI10"
/db_xref="GeneID:852876"
mRNA complement(<479910..>482666)
/gene="PMA1"
/locus_tag="YGL008C"
/gene_synonym="KTI10"
/product="H(+)-exporting P2-type ATPase PMA1"
/transcript_id="NM_001180873.1"
/db_xref="GeneID:852876"
CDS complement(479910..482666)
/gene="PMA1"
/locus_tag="YGL008C"
/gene_synonym="KTI10"
/EC_number="7.1.2.1"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836|PMID:11739806|PMID:12469340]"
/experiment="EXISTENCE:direct assay:GO:0008553 P-type
proton-exporting transporter activity [PMID:17912695]"
/experiment="EXISTENCE:direct assay:GO:0032126 eisosome
[PMID:15536122]"
/experiment="EXISTENCE:direct assay:GO:0045121 membrane
raft [PMID:10716729]"
/experiment="EXISTENCE:direct assay:GO:0055085
transmembrane transport [PMID:8396147]"
/experiment="EXISTENCE:direct assay:GO:1902600 proton
transmembrane transport [PMID:8396147]"
/experiment="EXISTENCE:mutant phenotype:GO:1902906
proteasome storage granule assembly [PMID:23690178]"
/experiment="EXISTENCE:mutant phenotype:GO:1904263
positive regulation of TORC1 signaling [PMID:29570051]"
/note="Plasma membrane P2-type H+-ATPase; pumps protons
out of cell; major regulator of cytoplasmic pH and plasma
membrane potential; long-lived hexameric protein
asymmetrically distributed at plasma membrane between
mother cells and buds; accumulates at high levels in
mother cells during aging, buds emerge with very low
levels of Pma1p, newborn cells have low levels of Pma1p;
Hsp30p plays a role in Pma1p regulation; interactions with
Std1p appear to propagate [GAR+]"
/codon_start=1
/product="H(+)-exporting P2-type ATPase PMA1"
/protein_id="NP_011507.1"
/db_xref="GeneID:852876"
/db_xref="SGD:S000002976"
/translation="MTDTSSSSSSSSASSVSAHQPTQEKPAKTYDDAASESSDDDDID
ALIEELQSNHGVDDEDSDNDGPVAAGEARPVPEEYLQTDPSYGLTSDEVLKRRKKYGL
NQMADEKESLVVKFVMFFVGPIQFVMEAAAILAAGLSDWVDFGVICGLLMLNAGVGFV
QEFQAGSIVDELKKTLANTAVVIRDGQLVEIPANEVVPGDILQLEDGTVIPTDGRIVT
EDCFLQIDQSAITGESLAVDKHYGDQTFSSSTVKRGEGFMVVTATGDNTFVGRAAALV
NKAAGGQGHFTEVLNGIGIILLVLVIATLLLVWTACFYRTNGIVRILRYTLGITIIGV
PVGLPAVVTTTMAVGAAYLAKKQAIVQKLSAIESLAGVEILCSDKTGTLTKNKLSLHE
PYTVEGVSPDDLMLTACLAASRKKKGLDAIDKAFLKSLKQYPKAKDALTKYKVLEFHP
FDPVSKKVTAVVESPEGERIVCVKGAPLFVLKTVEEDHPIPEDVHENYENKVAELASR
GFRALGVARKRGEGHWEILGVMPCMDPPRDDTAQTVSEARHLGLRVKMLTGDAVGIAK
ETCRQLGLGTNIYNAERLGLGGGGDMPGSELADFVENADGFAEVFPQHKYRVVEILQN
RGYLVAMTGDGVNDAPSLKKADTGIAVEGATDAARSAADIVFLAPGLSAIIDALKTSR
QIFHRMYSYVVYRIALSLHLEIFLGLWIAILDNSLDIDLIVFIAIFADVATLAIAYDN
APYSPKPVKWNLPRLWGMSIILGIVLAIGSWITLTTMFLPKGGIIQNFGAMNGIMFLQ
ISLTENWLIFITRAAGPFWSSIPSWQLAGAVFAVDIIATMFTLFGWWSENWTDIVTVV
RVWIWSIGIFCVLGGFYYEMSTSEAFDRLMNGKPMKEKKSTRSVEDFMAAMQRVSTQH
EKET"
gene complement(<482941..>483027)
/locus_tag="YGL007C-A"
/db_xref="GeneID:1466455"
mRNA complement(<482941..>483027)
/locus_tag="YGL007C-A"
/product="uncharacterized protein"
/transcript_id="NM_001184592.3"
/db_xref="GeneID:1466455"
CDS complement(482941..483027)
/locus_tag="YGL007C-A"
/note="hypothetical protein; identified by gene-trapping,
microarray-based expression analysis, and genome-wide
homology searching; deletion exhibits slow-growth
phenotype; computationally predicted to have a role in
cell budding"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878075.3"
/db_xref="GeneID:1466455"
/db_xref="SGD:S000028632"
/translation="MLPSISFDYIKRPNIVLFSNVLSLSSNI"
gene <483260..>483637
/gene="BRP1"
/locus_tag="YGL007W"
/db_xref="GeneID:852877"
mRNA <483260..>483637
/gene="BRP1"
/locus_tag="YGL007W"
/product="Brp1p"
/transcript_id="NM_001348838.1"
/db_xref="GeneID:852877"
CDS 483260..483637
/gene="BRP1"
/locus_tag="YGL007W"
/note="hypothetical protein; located in the upstream
region of PMA1; conserved among S. cerevisiae strains;
mediates the cycloheximide-induced degradation of
ubiquitinated Agp2p, a regulator of high affinity
polyamine and carnitine transport; null mutant is
polyamine resistant due to downregulation of PMA1"
/codon_start=1
/product="Brp1p"
/protein_id="NP_001335779.1"
/db_xref="GeneID:852877"
/db_xref="SGD:S000002975"
/translation="MMEKEGKKPERHMDNILKMSIPVKSMSEVSRPGSAALGNTDAMR
ASLRNSRRSFSGHVRLVLRLVYGFISAVIIAGSPLRDRVCFQITGKGQAKQSDVWVIP
AMPVYVSPFQEACFVFSPSAMVT"
rep_origin 484930..485157
/note="ARS719; Autonomously Replicating Sequence"
/db_xref="SGD:S000118459"
gene <485423..>485533
/locus_tag="YGL006W-A"
/db_xref="GeneID:1466456"
mRNA <485423..>485533
/locus_tag="YGL006W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184634.1"
/db_xref="GeneID:1466456"
CDS 485423..485533
/locus_tag="YGL006W-A"
/note="hypothetical protein; identified by SAGE"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878076.1"
/db_xref="GeneID:1466456"
/db_xref="SGD:S000028769"
/translation="MLIFIIHYHRHLALHLMGAFQKHSNSISPPPRKGFI"
gene <485921..>489442
/gene="PMC1"
/locus_tag="YGL006W"
/db_xref="GeneID:852878"
mRNA <485921..>489442
/gene="PMC1"
/locus_tag="YGL006W"
/product="calcium-transporting ATPase PMC1"
/transcript_id="NM_001180871.1"
/db_xref="GeneID:852878"
CDS 485921..489442
/gene="PMC1"
/locus_tag="YGL006W"
/EC_number="7.2.2.10"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane
[PMID:7507493|PMID:26928762|PMID:24124599]"
/experiment="EXISTENCE:genetic interaction:GO:0005388
P-type calcium transporter activity [PMID:7507493]"
/experiment="EXISTENCE:mutant phenotype:GO:0005388 P-type
calcium transporter activity [PMID:10431803]"
/experiment="EXISTENCE:mutant phenotype:GO:0006816 calcium
ion transport [PMID:15280017|PMID:7507493]"
/experiment="EXISTENCE:mutant phenotype:GO:0006874
intracellular calcium ion homeostasis [PMID:7507493]"
/experiment="EXISTENCE:mutant phenotype:GO:0055085
transmembrane transport [PMID:7507493]"
/note="Vacuolar Ca2+ ATPase involved in depleting cytosol
of Ca2+ ions; prevents growth inhibition by activation of
calcineurin in presence of elevated concentrations of
calcium; targeted to vacuole via AP-3 pathway; similar to
mammalian PMCA1a"
/codon_start=1
/product="calcium-transporting ATPase PMC1"
/protein_id="NP_011509.1"
/db_xref="GeneID:852878"
/db_xref="SGD:S000002974"
/translation="MSRQDENSALLANNENNKPSYTGNENGVYDNFKLSKSQLSDLHN
PKSIRSFVRLFGYESNSLFKYLKTDKNAGISLPEISNYRKTNRYKNYGDNSLPERIPK
SFLQLVWAAFNDKTMQLLTVAAVVSFVLGLYELWMQPPQYDPEGNKIKQVDWIEGVAI
MIAVFVVVLVSAANDYQKELQFAKLNKKKENRKIIVIRNDQEILISIHHVLVGDVISL
QTGDVVPADCVMISGKCEADESSITGESNTIQKFPVDNSLRDFKKFNSIDSHNHSKPL
DIGDVNEDGNKIADCMLISGSRILSGLGRGVITSVGINSVYGQTMTSLNAEPESTPLQ
LHLSQLADNISVYGCVSAIILFLVLFTRYLFYIIPEDGRFHDLDPAQKGSKFMNIFIT
SITVIVVAVPEGLPLAVTLALAFATTRMTKDGNLVRVLRSCETMGSATAVCSDKTGTL
TENVMTVVRGFPGNSKFDDSKSLPVSEQRKLNSKKVFEENCSSSLRNDLLANIVLNST
AFENRDYKKNDKNTNGSKNMSKNLSFLDKCKSRLSFFKKGNREDDEDQLFKNVNKGRQ
EPFIGSKTETALLSLARLSLGLQPGELQYLRDQPMEKFNIEKVVQTIPFESSRKWAGL
VVKYKEGKNKKPFYRFFIKGAAEIVSKNCSYKRNSDDTLEEINEDNKKETDDEIKNLA
SDALRAISVAHKDFCECDSWPPEQLRDKDSPNIAALDLLFNSQKGLILDGLLGIQDPL
RAGVRESVQQCQRAGVTVRMVTGDNILTAKAIARNCAILSTDISSEAYSAMEGTEFRK
LTKNERIRILPNLRVLARSSPEDKRLLVETLKGMGDVVAVTGDGTNDAPALKLADVGF
SMGISGTEVAREASDIILMTDDFSAIVNAIKWGRCVSVSIKKFIQFQLIVNITAVILT
FVSSVASSDETSVLTAVQLLWINLIMDTLAALALATDKPDPNIMDRKPRGRSTSLISV
STWKMILSQATLQLIVTFILHFYGPELFFKKHEDEITSHQQQQLNAMTFNTFVWLQFF
TMLVSRKLDEGDGISNWRGRISAANLNFFQDLGRNYYFLTIMAIIGSCQVLIMFFGGA
PFSIARQTKSMWITAVLCGMLSLIMGVLVRICPDEVAVKVFPAAFVQRFKYVFGLEFL
RKNHTGKHDDEEALLEESDSPESTAFY"
gene complement(<489708..>490547)
/gene="COG7"
/locus_tag="YGL005C"
/gene_synonym="COD5"
/db_xref="GeneID:852879"
mRNA complement(<489708..>490547)
/gene="COG7"
/locus_tag="YGL005C"
/gene_synonym="COD5"
/product="Golgi transport complex subunit COG7"
/transcript_id="NM_001180870.1"
/db_xref="GeneID:852879"
CDS complement(489708..490547)
/gene="COG7"
/locus_tag="YGL005C"
/gene_synonym="COD5"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0006891
intra-Golgi vesicle-mediated transport [PMID:11703943]"
/experiment="EXISTENCE:mutant phenotype:GO:0017119 Golgi
transport complex [PMID:11703943]"
/experiment="EXISTENCE:mutant phenotype:GO:0032258
cytoplasm to vacuole targeting by the Cvt pathway
[PMID:20065092]"
/note="Component of the conserved oligomeric Golgi
complex; a cytosolic tethering complex (Cog1p through
Cog8p) that functions in protein trafficking to mediate
fusion of transport vesicles to Golgi compartments"
/codon_start=1
/product="Golgi transport complex subunit COG7"
/protein_id="NP_011510.1"
/db_xref="GeneID:852879"
/db_xref="SGD:S000002973"
/translation="MVELTITGDDDDILSMFFDEEFVPHAFVDILLSNALNEDQIQTQ
SVSSLLLTRLDFYTKNLTKELESTIWNLDKLSQTLPRTWASSRYHKEAEQNDSSLYST
ESLKSSKLEYYLDTLASAVRALETGMHNVTEKLSDLDNENNRNTNVRQQLQSLMLIKE
RIEKVVYYLEQVRTVTNISTVRENNTTSTGTDLSITDFRTSLKALEDTIDESLSSAID
NEAKDETNKDLIGRIDSLSELKCLFKGLDKFFAEYSNFSESIKSKAQSYLSTKNIDDG
MIS"
gene complement(<490703..>491956)
/gene="RPN14"
/locus_tag="YGL004C"
/db_xref="GeneID:852880"
mRNA complement(<490703..>491956)
/gene="RPN14"
/locus_tag="YGL004C"
/product="Rpn14p"
/transcript_id="NM_001180869.3"
/db_xref="GeneID:852880"
CDS complement(490703..491956)
/gene="RPN14"
/locus_tag="YGL004C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:19446323]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:19446323]"
/experiment="EXISTENCE:direct assay:GO:0044183 protein
folding chaperone [PMID:19412159]"
/experiment="EXISTENCE:genetic interaction:GO:0070682
proteasome regulatory particle assembly
[PMID:19446322|PMID:19446323|PMID:19412159]"
/experiment="EXISTENCE:mutant phenotype:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:17499717]"
/experiment="EXISTENCE:mutant phenotype:GO:0070682
proteasome regulatory particle assembly [PMID:19446323]"
/note="Evolutionarily conserved 19S regulatory particle
assembly-chaperone; proteasome-interacting protein
involved in the assembly of the base subcomplex of the 19S
proteasome regulatory particle (RP); null mutants
accumulate ubiquitinated Gcn4p and display decreased 26S
proteasome stability; interacts with Rpt5p"
/codon_start=1
/product="Rpn14p"
/protein_id="NP_011511.3"
/db_xref="GeneID:852880"
/db_xref="SGD:S000002972"
/translation="MTKTITVAHIQYDFKAVLEENDENDDEFYINVDKNLNEIKEHKI
VVLGNSRGVDAGKGNTFEKVGSHLYKARLDGHDFLFNTIIRDGSKMLKRADYTAVDTA
KLQMRRFILGTTEGDIKVLDSNFNLQREIDQAHVSEITKLKFFPSGEALISSSQDMQL
KIWSVKDGSNPRTLIGHRATVTDIAIIDRGRNVLSASLDGTIRLWECGTGTTIHTFNR
KENPHDGVNSIALFVGTDRQLHEISTSKKNNLEFGTYGKYVIAGHVSGVITVHNVFSK
EQTIQLPSKFTCSCNSLTVDGNNANYIYAGYENGMLAQWDLRSPECPVGEFLINEGTP
INNVYFAAGALFVSSGFDTSIKLDIISDPESERPAIEFETPTFLVSNDDEVSQFCYVS
DDESNGEVLEVGKNNFCALYNLSNP"
gene complement(<492474..>494174)
/gene="CDH1"
/locus_tag="YGL003C"
/gene_synonym="HCT1"
/db_xref="GeneID:852881"
mRNA complement(<492474..>494174)
/gene="CDH1"
/locus_tag="YGL003C"
/gene_synonym="HCT1"
/product="Cdh1p"
/transcript_id="NM_001180868.1"
/db_xref="GeneID:852881"
CDS complement(492474..494174)
/gene="CDH1"
/locus_tag="YGL003C"
/gene_synonym="HCT1"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12456658]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:12456658]"
/experiment="EXISTENCE:direct assay:GO:0030332 cyclin
binding [PMID:11566880]"
/experiment="EXISTENCE:direct assay:GO:0045732 positive
regulation of protein catabolic process [PMID:17178718]"
/experiment="EXISTENCE:direct assay:GO:1990757 ubiquitin
ligase activator activity [PMID:15060174|PMID:19362536]"
/experiment="EXISTENCE:direct assay:GO:2000060 positive
regulation of ubiquitin-dependent protein catabolic
process [PMID:18172166]"
/experiment="EXISTENCE:genetic interaction:GO:0010697
negative regulation of mitotic spindle pole body
separation [PMID:16688214]"
/experiment="EXISTENCE:genetic interaction:GO:1903473
positive regulation of mitotic actomyosin contractile ring
contraction [PMID:19109423]"
/experiment="EXISTENCE:genetic interaction:GO:1905786
positive regulation of anaphase-promoting
complex-dependent catabolic process [PMID:9334304]"
/experiment="EXISTENCE:mutant phenotype:GO:0010697
negative regulation of mitotic spindle pole body
separation [PMID:16688214]"
/experiment="EXISTENCE:mutant phenotype:GO:0045842
positive regulation of mitotic metaphase/anaphase
transition [PMID:9831566]"
/experiment="EXISTENCE:mutant phenotype:GO:1902426
deactivation of mitotic spindle assembly checkpoint
[PMID:25025567]"
/experiment="EXISTENCE:mutant phenotype:GO:1903473
positive regulation of mitotic actomyosin contractile ring
contraction [PMID:19109423]"
/experiment="EXISTENCE:mutant phenotype:GO:1905786
positive regulation of anaphase-promoting
complex-dependent catabolic process [PMID:9334304]"
/experiment="EXISTENCE:physical interaction:GO:0005680
anaphase-promoting complex [PMID:9831566]"
/note="Activator of anaphase-promoting complex/cyclosome
(APC/C); antagonist of the spindle assembly checkpoint;
directs ubiquitination of cyclins resulting in mitotic
exit; targets the APC/C to specific substrates including:
Cdc20p, Ase1p, Cin8p, Fin1p and Clb5p; partially active in
metaphase, and fully active in anaphase; cell-cycle
regulated"
/codon_start=1
/product="Cdh1p"
/protein_id="NP_011512.1"
/db_xref="GeneID:852881"
/db_xref="SGD:S000002971"
/translation="MSTNLNPFMNNTPSSSPLKGSESKRVSKRPISSSSSASLLSSPS
RRSRPSTVYGDRYIPSRTDIDFNSIVSISSMASVPALNPSSTEDQVEYQKERQAHETY
NTLLKNELFGEMLSKDTVGSESSIDRIKNTRPSTRGNVHAENTTRHGYELERVSTPPP
EAAGLEEFSPHSTPVTPRRLFTSQQDEITRPSSNSVRGASLLTYQQRKGRRLSAASLL
QSQFFDSMSPVRPDSKQLLLSPGKQFRQIAKVPYRVLDAPSLADDFYYSLIDWSSTDV
LAVALGKSIFLTDNNTGDVVHLCDTENEYTSLSWIGAGSHLAVGQANGLVEIYDVMKR
KCIRTLSGHIDRVACLSWNNHVLTSGSRDHRILHRDVRMPDPFFETIESHTQEVCGLK
WNVADNKLASGGNDNVVHVYEGTSKSPILTFDEHKAAVKAMAWSPHKRGVLATGGGTA
DRRLKIWNVNTSIKMSDIDSGSQICNMVWSKNTNELVTSHGYSKYNLTLWDCNSMDPI
AILKGHSFRVLHLTLSNDGTTVVSGAGDETLRYWKLFDKPKAKVQPNSLIFDAFNQIR
"
gene <494517..>495167
/gene="ERP6"
/locus_tag="YGL002W"
/db_xref="GeneID:852882"
mRNA <494517..>495167
/gene="ERP6"
/locus_tag="YGL002W"
/product="Erp6p"
/transcript_id="NM_001180867.1"
/db_xref="GeneID:852882"
CDS 494517..495167
/gene="ERP6"
/locus_tag="YGL002W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:10359606]"
/note="Member of the p24 family involved in ER to Golgi
transport; similar to Emp24p and Erv25p; the authentic,
non-tagged protein is detected in highly purified
mitochondria in high-throughput studies; ERP6 has a
paralog, ERP1, that arose from the whole genome
duplication"
/codon_start=1
/product="Erp6p"
/protein_id="NP_011513.1"
/db_xref="GeneID:852882"
/db_xref="SGD:S000002970"
/translation="MLSHYIFLAFVLLPFRVSAFYFYGYGGDRKCFLKELSKDTLLKG
SYNLEVYDDKLADYALPSYNDYGIVIDVEEVFDNNHRVVHQQGSPSGDFSFLALESGE
YKICLQSRVNNWVGKTKTKLEIEFEVGFEAMLDMQRKETLESLHGKVSILNSKIVDIR
REQQLMREREESFRDISESVNSRAMWWTVTQVTLLIIICVWQMKSLRSFFVKQKVL"
gene complement(<495453..>496502)
/gene="ERG26"
/locus_tag="YGL001C"
/db_xref="GeneID:852883"
mRNA complement(<495453..>496502)
/gene="ERG26"
/locus_tag="YGL001C"
/product="sterol-4-alpha-carboxylate 3-dehydrogenase
(decarboxylating)"
/transcript_id="NM_001180866.1"
/db_xref="GeneID:852883"
CDS complement(495453..496502)
/gene="ERG26"
/locus_tag="YGL001C"
/EC_number="1.1.1.170"
/experiment="EXISTENCE:direct assay:GO:0000252
3-beta-hydroxysteroid dehydrogenase
[NAD(P)+]/C4-decarboxylase activity [PMID:9811880]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:12119386|PMID:26928762|PMID:11279045]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:12119386]"
/experiment="EXISTENCE:direct assay:GO:0006696 ergosterol
biosynthetic process [PMID:9811880]"
/experiment="EXISTENCE:mutant phenotype:GO:0006696
ergosterol biosynthetic process [PMID:9811880]"
/experiment="EXISTENCE:mutant phenotype:GO:0102175
3-beta-hydroxysteroid dehydrogenase
(NAD+)/C4-decarboxylase activity [PMID:9811880]"
/note="C-3 sterol dehydrogenase; catalyzes the second of
three steps required to remove two C-4 methyl groups from
an intermediate in ergosterol biosynthesis; human homolog
NSDHL implicated in CK syndrome, and can complement yeast
null mutant; molecular target of natural product and
antifungal compound FR171456"
/codon_start=1
/product="sterol-4-alpha-carboxylate 3-dehydrogenase
(decarboxylating)"
/protein_id="NP_011514.1"
/db_xref="GeneID:852883"
/db_xref="SGD:S000002969"
/translation="MSKIDSVLIIGGSGFLGLHLIQQFFDINPKPDIHIFDVRDLPEK
LSKQFTFNVDDIKFHKGDLTSPDDMENAINESKANVVVHCASPMHGQNPDIYDIVNVK
GTRNVIDMCKKCGVNILVYTSSAGVIFNGQDVHNADETWPIPEVPMDAYNETKAIAED
MVLKANDPSSDFYTVALRPAGIFGPGDRQLVPGLRQVAKLGQSKFQIGDNNNLFDWTY
AGNVADAHVLAAQKLLDPKTRTAVSGETFFITNDTPTYFWALARTVWKADGHIDKHVI
VLKRPVAICAGYLSEWVSKMLGKEPGLTPFRVKIVCAYRYHNIAKAKKLLGYTPRVGI
EEGINKTLAWMDEGL"
centromere complement(496920..497038)
/note="CEN7; Chromosome VII centromere"
/db_xref="SGD:S000006468"
centromere complement(496920..496944)
/note="CEN7_CDEIII of CEN7"
centromere complement(496945..497028)
/note="CEN7_CDEII of CEN7"
centromere complement(497029..497038)
/note="CEN7_CDEI of CEN7"
gene complement(<497133..>498034)
/gene="EFM5"
/locus_tag="YGR001C"
/gene_synonym="AML1"
/db_xref="GeneID:852884"
mRNA complement(join(<497133..497365,497459..497937,
498000..>498034))
/gene="EFM5"
/locus_tag="YGR001C"
/gene_synonym="AML1"
/product="protein-lysine N-methyltransferase"
/transcript_id="NM_001181130.3"
/db_xref="GeneID:852884"
CDS complement(join(497133..497365,497459..497937,
498000..498034))
/gene="EFM5"
/locus_tag="YGR001C"
/gene_synonym="AML1"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0016279
protein-lysine N-methyltransferase activity
[PMID:26545399]"
/experiment="EXISTENCE:direct assay:GO:0018023
peptidyl-lysine trimethylation [PMID:26545399]"
/experiment="EXISTENCE:mutant phenotype:GO:0016279
protein-lysine N-methyltransferase activity
[PMID:25446118]"
/experiment="EXISTENCE:mutant phenotype:GO:0018023
peptidyl-lysine trimethylation [PMID:25446118]"
/note="S-adenosylmethionine-dependent lysine
methyltransferase; involved in the trimethylation of eEF1A
(Tef1p/Tef2p) at lysine 79; green fluorescent protein
(GFP)-fusion protein localizes to the cytoplasm; required
for replication of Brome mosaic virus in budding yeast;
expresses a circular RNA; originally misclassified as a
N-6-adenine specific DNA methyltransferase based on
sequence similarity; both Efm5p and human ortholog N6AMT2
can methylate eEF1a from either species in vitro"
/codon_start=1
/product="protein-lysine N-methyltransferase"
/protein_id="NP_011515.4"
/db_xref="GeneID:852884"
/db_xref="SGD:S000003233"
/translation="MSDSDSDSDYELTLSANALAALEEFKREEQQHQEAFQKLYDETD
EDFQKKKKEEGMKLFKEDWQLSQFWYSDDTAAILADAILEGADENTVIAIVSAPSVYA
AIQKKPTNEIPTEHIYLFEFDKRFELLAGRDHFFFYDYNKPLDFSDEIKGKVDRLLID
PPFLNEDCQTKSSITAKCLLAPNDNSKTKKGVFKHRLISCTGERMSEVISKVYSDTRI
TTFLPEHSNGLSNEFRCYANFECSSWKFAS"
gene complement(<498476..>499906)
/gene="SWC4"
/locus_tag="YGR002C"
/gene_synonym="EAF2; GOD1"
/db_xref="GeneID:852885"
mRNA complement(<498476..>499906)
/gene="SWC4"
/locus_tag="YGR002C"
/gene_synonym="EAF2; GOD1"
/product="Swc4p"
/transcript_id="NM_001181131.1"
/db_xref="GeneID:852885"
CDS complement(498476..499906)
/gene="SWC4"
/locus_tag="YGR002C"
/gene_synonym="EAF2; GOD1"
/experiment="EXISTENCE:direct assay:GO:0000812 Swr1
complex [PMID:14645854|PMID:14690608]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095|PMID:14690591]"
/experiment="EXISTENCE:direct assay:GO:0006281 DNA repair
[PMID:16135807]"
/experiment="EXISTENCE:direct assay:GO:0006338 chromatin
remodeling [PMID:14645854]"
/experiment="EXISTENCE:direct assay:GO:0035267 NuA4
histone acetyltransferase complex
[PMID:15485911|PMID:15353583]"
/experiment="EXISTENCE:mutant phenotype:GO:0051276
chromosome organization [PMID:18439903]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:15302830]"
/experiment="EXISTENCE:physical interaction:GO:0006338
chromatin remodeling [PMID:14690608]"
/note="Component of the Swr1p complex that incorporates
Htz1p into chromatin; component of the NuA4 histone
acetyltransferase complex; associates with and protects
nucleosome-free regions of the genome including rDNA, tDNA
and telomeric loci, contributing to genome integrity"
/codon_start=1
/product="Swc4p"
/protein_id="NP_011516.1"
/db_xref="GeneID:852885"
/db_xref="SGD:S000003234"
/translation="MSSSDIFDVLNIKQKSRSPTNGQVSVPSSSAANRPKPQVTGMQR
ELFNLLGENQPPVVIKSGNNFKEKMLSTSKPSPWSFVEFKANNSVTLRHWVKGSKELI
GDTPKESPYSKFNQHLSIPSFTKEEYEAFMNENEGTQKSVESEKNHNENFTNEKKDES
KNSWSFEEIEYLFNLCKKYDLRWFLIFDRYSYNNSRTLEDLKEKFYYTCRNYFKASDP
SNPLLSSLNFSAEKEIERKKYLQRLLSRSAAEIAEEEALVVESKKFEMAAKRTLAERE
SLLRLLDSPHSDQTITQYLTSQGMSQLYNALLADKTRKRKHDLNIPENPWMKQQQQFA
QHRQLQQLNVKKSEVKENLSPKKTKRQRQEMQTALKRKSESAYAEQLLKDFNSDERKA
LGVITHGEKLSPGVYLRSTKLSTFKPALQNKILAILQELSLPSRPVMPSFDVMERQEE
LLKKINTLIDLKKHVDKYEAGMSITK"
gene <500132..>502366
/gene="CUL3"
/locus_tag="YGR003W"
/db_xref="GeneID:852886"
mRNA <500132..>502366
/gene="CUL3"
/locus_tag="YGR003W"
/product="cullin CUL3"
/transcript_id="NM_001181132.1"
/db_xref="GeneID:852886"
CDS 500132..502366
/gene="CUL3"
/locus_tag="YGR003W"
/experiment="EXISTENCE:direct assay:GO:0004842
ubiquitin-protein transferase activity
[PMID:16675952|PMID:12676951]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0031463 Cul3-RING
ubiquitin ligase complex [PMID:16675952]"
/experiment="EXISTENCE:direct assay:GO:0034644 cellular
response to UV [PMID:16675952]"
/experiment="EXISTENCE:mutant phenotype:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:17296727]"
/experiment="EXISTENCE:mutant phenotype:GO:0031463
Cul3-RING ubiquitin ligase complex [PMID:17296727]"
/note="Ubiquitin-protein ligase; forms a complex with
Elc1p that polyubiquitylates monoubiquitylated RNA
polymerase II to trigger its proteolysis; cullin family
member with similarity to Cdc53p and human CUL3"
/codon_start=1
/product="cullin CUL3"
/protein_id="NP_011517.1"
/db_xref="GeneID:852886"
/db_xref="SGD:S000003235"
/translation="MITNKKIKISVPEKLGLSEESFEESWETVKYAIDHIYSDDMADL
SFEQVYKTIYTIVLNKKGPILYNRLKDYLIQKLALLRETIVKDNTHDYEFLGTMARLW
EVQCHCFKITGDLMMYMDKVYCKPNRCMEVYDMCLDLFRIEILQKCSSSLISALISDI
ERIRNLGSVDSEHTSLWKVLIGMMETLHDNRDSFFLTDFEPVLISATEEYYNKAIDIE
LLTPIESLEKIRKLRQFESMLDSSFLNVDSHNKLKTVLENVLIWGKLSDIIEDLTHEA
MVISNGKLLQEIYDLSSEEKYRVTVIESIKSYINKNAINIPFNEGNRKKGQNAITWSS
EIVELYRSQHSFLESIDFGSVRLNNLTGDVSNAILGDVFSMYFSKEGALPSEYLSTYV
DHCMKRTKEKDAEIVKIKQDLLDSTKLIGLLTEKDIFEKIYKKQLSRRLLQQRSLVEI
EKWMVQMIKKVLGTFFTSKLEIMLRDISLSSEMYQAFQHSTINSIEYLSFAPQVLTRT
SWPFQSTNPIDEGISLPPRMSQILAGFEGYYSLKYKERVLKWAHHLSVIEIGCQFNSG
YYEISFSVYAGVIFLLFEDYEELTLGEIYELTHIPIDDVKSLVMSMSTIPRCKILKKS
SSSGNMKFSVNYFFSSPNRKVKVPVIACPLPSQKSDNLATASSVDTYDNEIVMELSAI
IVRIMKTEGKLSHQQLLERTTKRTQSRLSLTPSILKRSIQLLIEKEYIQRNADDPSYY
HYLS"
gene <502938..>504326
/gene="PEX31"
/locus_tag="YGR004W"
/db_xref="GeneID:852887"
mRNA <502938..>504326
/gene="PEX31"
/locus_tag="YGR004W"
/product="peroxisome biogenesis protein"
/transcript_id="NM_001181133.1"
/db_xref="GeneID:852887"
CDS 502938..504326
/gene="PEX31"
/locus_tag="YGR004W"
/experiment="EXISTENCE:direct assay:GO:0005778 peroxisomal
membrane [PMID:14617799]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0071561
nucleus-vacuole junction [PMID:41132095]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:1900063
regulation of peroxisome organization [PMID:27872254]"
/experiment="EXISTENCE:mutant phenotype:GO:0007031
peroxisome organization [PMID:14617799]"
/note="Peroxisomal integral membrane protein; negative
regulator of peroxisome size; partial functional
redundancy with Pex30p and Pex32p; may act downstream of
Pex28p and Pex29p; involved in metabolic adaptation of the
nucleus-vacuole junction (NVJ); enriched at the NVJ in
high glucose conditions; PEX31 has a paralog, PEX30, that
arose from the whole genome duplication"
/codon_start=1
/product="peroxisome biogenesis protein"
/protein_id="NP_011518.1"
/db_xref="GeneID:852887"
/db_xref="SGD:S000003236"
/translation="MSEINNENLEPTSSTVAESTESKNKHIRSALRKRRGKLSAQTYE
EDQEAILSSPLLTSTPKTVSRSLVRLYPYLIVVDNFLSIITWSNDNVSANLLGIFLFT
VCVLYFGFITRYFGHLMIVGIIWVYLLIDKHVQETMASCPSLDDIIHVMDRVSMKSSA
VLSPITILSAQDVRRLLFTIAFLSPVYIFLTVFVLSPNYLMLIGGLYVLTYHSKLIRR
MRRYLWKFRVVRLLVFFITGLDLGGPDNNRRLFASVNKKIRSFVWNEVGNTSNTKKTV
LFKVALFENQRRWLGIGWTSTMLSYERASWTDEFLNTSPSPEVFTLPEEQSGMAWEWH
DKDWMLDLTNDGIIQLPASAAKTKVKPGADEGFIYYDNTWNNPSATDTYKKYTRRRRW
IRTATVTTTYDDEPTVEKATPNSHALKSEENNRVRKRKVSFSTANEVHIIPSSDSSKL
IQISDVSMSPSL"
gene complement(<504657..>505859)
/gene="TFG2"
/locus_tag="YGR005C"
/db_xref="GeneID:852888"
mRNA complement(<504657..>505859)
/gene="TFG2"
/locus_tag="YGR005C"
/product="transcription factor IIF subunit TFG2"
/transcript_id="NM_001181134.3"
/db_xref="GeneID:852888"
CDS complement(504657..505859)
/gene="TFG2"
/locus_tag="YGR005C"
/experiment="EXISTENCE:direct assay:GO:0000993 RNA
polymerase II complex binding [PMID:14749386]"
/experiment="EXISTENCE:direct assay:GO:0005674
transcription factor TFIIF complex
[PMID:7995524|PMID:12242279]"
/experiment="EXISTENCE:direct assay:GO:0006367
transcription initiation at RNA polymerase II promoter
[PMID:1331084|PMID:18362165]"
/experiment="EXISTENCE:direct assay:GO:0006368
transcription elongation by RNA polymerase II
[PMID:18362165]"
/experiment="EXISTENCE:mutant phenotype:GO:0001174
transcriptional start site selection at RNA polymerase II
promoter [PMID:20033062|PMID:15572698]"
/note="TFIIF (Transcription Factor II) middle subunit;
involved in both transcription initiation and elongation
of RNA polymerase II; homologous to human RAP30"
/codon_start=1
/product="transcription factor IIF subunit TFG2"
/protein_id="NP_011519.3"
/db_xref="GeneID:852888"
/db_xref="SGD:S000003237"
/translation="MSSGSAGAPALSNNSTNSVAKEKSGNISGDEYLSQEEEVFDGND
IENNETKVYEESLDLDLERSNRQVWLVRLPMFLAEKWRDRNNLHGQELGKIRINKDGS
KITLLLNENDNDSIPHEYDLELTKKVVENEYVFTEQNLKKYQQRKKELEADPEKQRQA
YLKKQEREEELKKKQQQQKRRNNRKKFNHRVMTDRDGRDRYIPYVKTIPKKTAIVGTV
CHECQVMPSMNDPNYHKIVEQRRNIVKLNNKERITTLDETVGVTMSHTGMSMRSDNSN
FLKVGREKAKSNIKSIRMPKKEILDYLFKLFDEYDYWSLKGLKERTRQPEAHLKECLD
KVATLVKKGPYAFKYTLRPEYKKLKEEERKATLGELADEQTGSAGDNAQGDAEADLED
EIEMEDVV"
gene <506070..>506825
/gene="PRP18"
/locus_tag="YGR006W"
/db_xref="GeneID:852889"
mRNA <506070..>506825
/gene="PRP18"
/locus_tag="YGR006W"
/product="mRNA splicing protein PRP18"
/transcript_id="NM_001181135.1"
/db_xref="GeneID:852889"
CDS 506070..506825
/gene="PRP18"
/locus_tag="YGR006W"
/experiment="EXISTENCE:direct assay:GO:0000350 generation
of catalytic spliceosome for second transesterification
step [PMID:12212850]"
/experiment="EXISTENCE:direct assay:GO:0000386 second
spliceosomal transesterification activity [PMID:12212850]"
/experiment="EXISTENCE:direct assay:GO:0005682 U5 snRNP
[PMID:8474454]"
/experiment="EXISTENCE:direct assay:GO:0046540 U4/U6 x U5
tri-snRNP complex [PMID:8474454]"
/experiment="EXISTENCE:direct assay:GO:0071021 U2-type
post-spliceosomal complex [PMID:12212850]"
/experiment="EXISTENCE:genetic interaction:GO:0000386
second spliceosomal transesterification activity
[PMID:17626844]"
/experiment="EXISTENCE:genetic interaction:GO:0071028
nuclear mRNA surveillance [PMID:14718167]"
/experiment="EXISTENCE:mutant phenotype:GO:0000398 mRNA
splicing, via spliceosome [PMID:12403466]"
/experiment="EXISTENCE:mutant phenotype:GO:0071028 nuclear
mRNA surveillance [PMID:14718167]"
/note="Splicing factor and component of snRNP U5; factor
involved in the positioning of the 3' splice site during
the second catalytic step of splicing; interacts with
Slu7p"
/codon_start=1
/product="mRNA splicing protein PRP18"
/protein_id="NP_011520.2"
/db_xref="GeneID:852889"
/db_xref="SGD:S000003238"
/translation="MDLDLASILKGEISKKKKELANSKGVQPPCTEKFQPHESANIDE
TPRQVEQESTDEENLSDNQSDDIRTTISKLENRPERIQEAIAQDKTISVIIDPSQIGS
TEGKPLLSMKCNLYIHEILSRWKASLEAYHPELFLDTKKALFPLLLQLRRNQLAPDLL
ISLATVLYHLQQPKEINLAVQSYMKLSIGNVAWPIGVTSVGIHARSAHSKIQGGRNAA
NIMIDERTRLWITSIKRLITFEEWYTSNHDSLA"
gene <506969..>507940
/gene="ECT1"
/locus_tag="YGR007W"
/gene_synonym="MUQ1"
/db_xref="GeneID:852890"
mRNA <506969..>507940
/gene="ECT1"
/locus_tag="YGR007W"
/gene_synonym="MUQ1"
/product="ethanolamine-phosphate cytidylyltransferase"
/transcript_id="NM_001181136.1"
/db_xref="GeneID:852890"
CDS 506969..507940
/gene="ECT1"
/locus_tag="YGR007W"
/gene_synonym="MUQ1"
/EC_number="2.7.7.14"
/experiment="EXISTENCE:direct assay:GO:0004306
ethanolamine-phosphate cytidylyltransferase activity
[PMID:8982874]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0004306
ethanolamine-phosphate cytidylyltransferase activity
[PMID:8982874]"
/experiment="EXISTENCE:mutant phenotype:GO:0006646
phosphatidylethanolamine biosynthetic process
[PMID:8982874]"
/note="Ethanolamine-phosphate cytidylyltransferase;
catalyzes the second step of phosphatidylethanolamine
biosynthesis; involved in the maintenance of plasma
membrane; similar to mammalian CTP: phosphocholine
cytidylyl-transferases; inability of the null mutant to
synthesize phosphatidylethanolamine and
phosphatidylcholine from ethanolamine is functionally
complemented by human PCYT2"
/codon_start=1
/product="ethanolamine-phosphate cytidylyltransferase"
/protein_id="NP_011521.1"
/db_xref="GeneID:852890"
/db_xref="SGD:S000003239"
/translation="MTVNLDPDKVWIDGCFDFTHHGHAGAILQARRTVSKENGKLFCG
VHTDEDIQHNKGTPVMNSSERYEHTRSNRWCSEVVEAAPYVTDPNWMDKYQCQYVVHG
DDITIDANGEDCYKLVKEMGRFKVVKRTYGVSTTEIIHRILTKKSLPPTHPDYYPTTQ
ELSFYSVAQDAVSKHCYVFQRDLDNVLVNGGYKFDAEDCVYVDGDFDLFHMGDIDQLR
KLKMDLHPDKKLIVGITTSDYSSTIMTMKERVLSVLSCKYVDAVIIDADATSMSQYNC
EKYHIGTAVLTAAGKFSEYLTKELIVKRVESQREVYIARNQKKGMSI"
gene complement(<508110..>508364)
/gene="STF2"
/locus_tag="YGR008C"
/db_xref="GeneID:852891"
mRNA complement(<508110..>508364)
/gene="STF2"
/locus_tag="YGR008C"
/product="ATPase-stabilizing factor family protein"
/transcript_id="NM_001181137.1"
/db_xref="GeneID:852891"
CDS complement(508110..508364)
/gene="STF2"
/locus_tag="YGR008C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22442684]"
/experiment="EXISTENCE:mutant phenotype:GO:0071465
cellular response to desiccation [PMID:22442684]"
/note="Protein involved in resistance to desiccation
stress; Stf2p exhibits antioxidant properties, and its
overexpression prevents ROS accumulation and apoptosis;
binds to F0 sector of mitochondrial F1F0 ATPase in vitro
and may modulate the inhibitory action of Inh1p and Stf1p;
protein abundance increases in response to DNA replication
stress; STF2 has a paralog, TMA10, that arose from the
whole genome duplication"
/codon_start=1
/product="ATPase-stabilizing factor family protein"
/protein_id="NP_011522.1"
/db_xref="GeneID:852891"
/db_xref="SGD:S000003240"
/translation="MTRTNKWTEREGKADPKYFSHTGNYGESPNHIKKQGSGKGNWGK
PGDEIDDLIDNGEIPPVFKKDRRGSNLQSHEQKFENVQKE"
rep_origin 508729..508977
/note="ARS720; Autonomously Replicating Sequence"
/db_xref="SGD:S000118460"
gene complement(<509047..>511002)
/gene="SEC9"
/locus_tag="YGR009C"
/gene_synonym="HSS7"
/db_xref="GeneID:852892"
mRNA complement(<509047..>511002)
/gene="SEC9"
/locus_tag="YGR009C"
/gene_synonym="HSS7"
/product="Sec9p"
/transcript_id="NM_001181138.3"
/db_xref="GeneID:852892"
CDS complement(509047..511002)
/gene="SEC9"
/locus_tag="YGR009C"
/gene_synonym="HSS7"
/experiment="EXISTENCE:direct assay:GO:0005484 SNAP
receptor activity [PMID:16339720]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0006906 vesicle
fusion [PMID:11001046]"
/experiment="EXISTENCE:direct assay:GO:0031201 SNARE
complex [PMID:9195974]"
/experiment="EXISTENCE:genetic interaction:GO:0006887
exocytosis [PMID:12913108]"
/note="t-SNARE protein required for secretory
vesicle-plasma membrane fusion; similar to but not
functionally redundant with Spo20p; interacts non-exocyst
bound Sec6p; SNAP-25 homolog"
/codon_start=1
/product="Sec9p"
/protein_id="NP_011523.3"
/db_xref="GeneID:852892"
/db_xref="SGD:S000003241"
/translation="MGLKKFFKIKPPEEATPEQNKDTLMELGISVKNPSKKRKEKFAA
YGKFANDKAEDKVYAPPGYEQYARPQDELEDLNASPLDANANEATAGSNRGSSGTQDL
GNGAESNSMQDPYAIENDDYRYDDDPYARFQANKSNGRGSVNAAPYGDYGGGYNGTSL
NSYNNDGPYSNQNTSNSWVNANGRNSLNHSNSTLNVGPSRQTRQPPVSTSTNSLSLDQ
RSPLANPMQEKRNPYADMNSYGGAYDSNTNRSSGTRQGSSKNANPYASMANDSYSNGN
LNRSANPYSSRSVRQPQSQQAPMTYTPSFIASDEAARNSEVDLNEEPRTGEFDFEEVY
ADKSAENRAALDEPDLNAVMTNEDSIDLNASEVDHSSRQQQQQQWFMDEQQQQQQHFN
ATNNQYGDQRGYKTFEEIQKEEEARQQQEEDEAVDEIKQEIKFTKQSSVASTRNTLKM
AQDAERAGMNTLGMLGHQSEQLNNVEGNLDLMKVQNKVADEKVAELKKLNRSILAVHV
SNPFNSKRRRREREEQLKNRKIEEKLMREQTSQQLSQSTQRIEGAMNANNNISEVRER
YQRKNVLEKAKRYQFENDEEDDEMELEIDRNLDQIQQVSNRLKKMALTTGKELDSQQK
RLNNIEESTDDLDINLHMNTNRLAGIR"
gene <511545..>512732
/gene="NMA2"
/locus_tag="YGR010W"
/db_xref="GeneID:852893"
mRNA <511545..>512732
/gene="NMA2"
/locus_tag="YGR010W"
/product="nicotinamide-nucleotide adenylyltransferase
NMA2"
/transcript_id="NM_001181139.1"
/db_xref="GeneID:852893"
CDS 511545..512732
/gene="NMA2"
/locus_tag="YGR010W"
/EC_number="2.7.7.1"
/EC_number="2.7.7.18"
/experiment="EXISTENCE:direct assay:GO:0000309
nicotinamide-nucleotide adenylyltransferase activity
[PMID:21621500]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11884393]"
/experiment="EXISTENCE:direct assay:GO:0009435 NAD+
biosynthetic process [PMID:21621500]"
/note="Nicotinic acid mononucleotide adenylyltransferase;
catalyzes the transfer of the adenylyl moiety of ATP to
nicotinamide mononucleotide to form NAD; involved in de
novo and salvage synthesis of NAD(+); homolog of human
NMNAT; NMA2 has a paralog, NMA1, that arose from the whole
genome duplication"
/codon_start=1
/product="nicotinamide-nucleotide adenylyltransferase
NMA2"
/protein_id="NP_011524.1"
/db_xref="GeneID:852893"
/db_xref="SGD:S000003242"
/translation="MDPTKAPDFKPPQPNEELQPPPDPTHTIPKSGPIVPYVLADYNS
SIDAPFNLDIYKTLSSRKKNANSSNRMDHIPLNTSDFQPLSRDVSSEEESEGQSNGID
ATLQDVTMTGNLGVLKSQIADLEEVPHTIVRQARTIEDYEFPVHRLTKKLQDPEKLPL
IIVACGSFSPITYLHLRMFEMALDDINEQTRFEVVGGYFSPVSDNYQKRGLAPAYHRV
RMCELACERTSSWLMVDAWESLQSSYTRTAKVLDHFNHEINIKRGGIMTVDGEKMGVK
IMLLAGGDLIESMGEPHVWADSDLHHILGNYGCLIVERTGSDVRSFLLSHDIMYEHRR
NILIIKQLIYNDISSTKVRLFIRRGMSVQYLLPNSVIRYIQEYNLYINQSEPVKQVLD
SKE"
gene <513159..>514340
/gene="MCY1"
/locus_tag="YGR012W"
/gene_synonym="TCD3"
/db_xref="GeneID:852895"
mRNA <513159..>514340
/gene="MCY1"
/locus_tag="YGR012W"
/gene_synonym="TCD3"
/product="putative cysteine synthase"
/transcript_id="NM_001181141.1"
/db_xref="GeneID:852895"
CDS 513159..514340
/gene="MCY1"
/locus_tag="YGR012W"
/gene_synonym="TCD3"
/EC_number="2.5.1.47"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:16823961|PMID:14576278|PMID:24769239|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:16407407]"
/note="Putative cysteine synthase; localized to the
mitochondrial outer membrane; interacts with TCD1 and
TCD2, likely forming a complex"
/codon_start=1
/product="putative cysteine synthase"
/protein_id="NP_011526.1"
/db_xref="GeneID:852895"
/db_xref="SGD:S000003244"
/translation="MSCSQNKTSVSLAWRECISIASVLIGAYASYKYYKLFKTRDIPR
PKEGVEELIGNTPLVKIRSLTKATGVNIYAKLELCNPAGSAKDRVALNIIKTAEELGE
LVRGEPGWVFEGTSGSTGISIAVVCNALGYRAHISLPDDTSLEKLALLESLGATVNKV
KPASIVDPNQYVNAAKKACNELKKSGNGIRAVFADQFENEANWKVHYQTTGPEIAHQT
KGNIDAFIAGCGTGGTITGVAKFLKERAKIPCHVVLADPQGSGFYNRVNYGVMYDYVE
KEGTRRRHQVDTIVEGIGLNRITHNFHMGEKFIDESIRVNDNQAIRMAKYLSVNDGLF
VGSSTAINAVAAIQVAKTLPHGSNIVIIACDSGSRHLSKFWKEAKEIDHDVSLEEVIN
I"
gene <514554..>516416
/gene="SNU71"
/locus_tag="YGR013W"
/db_xref="GeneID:852896"
mRNA <514554..>516416
/gene="SNU71"
/locus_tag="YGR013W"
/product="Snu71p"
/transcript_id="NM_001181142.1"
/db_xref="GeneID:852896"
CDS 514554..516416
/gene="SNU71"
/locus_tag="YGR013W"
/experiment="EXISTENCE:direct assay:GO:0000398 mRNA
splicing, via spliceosome [PMID:9630245]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:9630245]"
/experiment="EXISTENCE:direct assay:GO:0005685 U1 snRNP
[PMID:9630245]"
/experiment="EXISTENCE:direct assay:GO:0071004 U2-type
prespliceosome [PMID:16618970]"
/note="Component of U1 snRNP required for mRNA splicing
via spliceosome; yeast specific, no metazoan counterpart"
/codon_start=1
/product="Snu71p"
/protein_id="NP_011527.1"
/db_xref="GeneID:852896"
/db_xref="SGD:S000003245"
/translation="MRDIVFVSPQLYLSSQEGWKSDSAKSGFIPILKNDLQRFQDSLK
HIVDARNSLSETLLNSNDDGSIHNSDQNTGLNKDKEASIADNNSANKCATSSSRYQEL
KQFLPISLDQQIHTVSLQGVSSSFSRGQIESLLDHCLNLALTETQSNSALKVEAWSSF
SSFLDTQDIFIRFSKVDEDEAFVNTLNYCKALFAFIRKLHEDFKIELHLDLNTKEYVE
DRTGTIPSVKPEKASEFYSVFKNIEDQTDERNSKKEQLDDSSTQYKVDTNTLSDLPSD
ALDQLCKDIIEFRTKVVSIEKEKKMKSTYEESRRQRHQMQKVFDQIRKNHSGAKGSAN
TEEEDTNMEDEDEEDDTEDDLALEKRKEERDLEESNRRYEDMLHQLHSNTEPKIKSIR
ADIMSAENYEEHLEKNRSLYLKELLHLANDVHYDHHRSFKEQEERRDEEDRAKNGNAK
ELAPIQLSDGKAISAGKAAAITLPEGTVKSENYNADKNVSESSEHVKIKFDFKKAIDH
SVESSSEDEGYRESELPPTKPSERSAAEDRLPFTADELNIRLTNLKESRYVDELVREF
LGVYEDELVEYILENIRVNQSKQALLNELRETFDEDGETIADRLWSRKEFRLGT"
gene <516943..>520863
/gene="MSB2"
/locus_tag="YGR014W"
/db_xref="GeneID:852897"
mRNA <516943..>520863
/gene="MSB2"
/locus_tag="YGR014W"
/product="Msb2p"
/transcript_id="NM_001181143.3"
/db_xref="GeneID:852897"
CDS 516943..520863
/gene="MSB2"
/locus_tag="YGR014W"
/experiment="EXISTENCE:direct assay:GO:0005576
extracellular region [PMID:18591427]"
/experiment="EXISTENCE:direct assay:GO:0030427 site of
polarized growth [PMID:15256499]"
/experiment="EXISTENCE:genetic interaction:GO:0006972
hyperosmotic response [PMID:17627274]"
/experiment="EXISTENCE:genetic interaction:GO:0007232
osmosensory signaling pathway via Sho1 osmosensor
[PMID:17627274]"
/experiment="EXISTENCE:genetic interaction:GO:0030010
establishment of cell polarity [PMID:2690082]"
/experiment="EXISTENCE:mutant phenotype:GO:0001402 signal
transduction involved in filamentous growth
[PMID:15256499|PMID:18591427]"
/experiment="EXISTENCE:mutant phenotype:GO:0005034
osmosensor activity [PMID:12052881]"
/experiment="EXISTENCE:mutant phenotype:GO:0005886 plasma
membrane [PMID:12052881]"
/experiment="EXISTENCE:mutant phenotype:GO:0006970
response to osmotic stress [PMID:12052881]"
/experiment="EXISTENCE:mutant phenotype:GO:0030010
establishment of cell polarity [PMID:19439450]"
/note="Mucin family member involved in various signaling
pathways; functions as osmosensor in the Sho1p-mediated
HOG pathway; functions in Cdc42p- and MAP kinase-dependent
filamentous growth signaling pathway; processed into
secreted and cell-associated forms by aspartyl protease
Yps1p; potential Cdc28p substrate"
/codon_start=1
/product="Msb2p"
/protein_id="NP_011528.3"
/db_xref="GeneID:852897"
/db_xref="SGD:S000003246"
/translation="MQFPFACLLSTLVISGSLARASPFDFIFGNGTQQAQSQSESQGQ
VSFTNEASQDSSTTSLVTAYSQGVHSHQSATIVSATISSLPSTWYDASSTSQTSVSYA
SQESDYAVNQNSWSASTNQLPSTSTTSYYAPTFSTSADFAASSVNAASDVSTASVPID
TSANSIPFTTTSNIETTTSAPLTSDTPLISTSTMSAADNVFSSANPISASLTTTDSSE
SFDQTSTAGAIPVQSSADFSSSSEILVQSSADFSSPSSPTTTDISLSAAPLQTSESSS
FTTASAALPVSSTDVDGSSASPVVSMSAAGQIASSSSTDNPTMSETFSLTSTEVDGSD
VSSTVSALLSAPFLQTSTSNSFSIVSPSVSFVPSQSSSDVASSSTANVVSSSFSDIPP
QTSTSGSVVSVAQSASALAFQSSTEVYGASASSTMSSLLSTTSLQSTTLDSSSLASSS
ASSSDLTDYGVSSTASIPLLSASEQASTSSSFSVVSPSVSFVPSQSSSDVASTSAPSV
VSSSFSYTSLQAGGSSMTNPSSSTIVYSSSTGSSEESAASTASATLSGSSSTYMAGNL
QSQPPSTSSLLSESQATSTSAVLASSSVSTTSPYTTAGGASTEASSLISSTSAETSQV
SYSQSTTALQTSSFASSSTTEGSETSSQGFSTSSVLVQMPSSISSEFSPSQTTTQMNS
ASSSSQYTISSTGILSQVSDTSVSYTTSSSSVSQVSDTPVSYTTSSSSVSQVSDTPVS
YTTSSSSVSQVSDTPVSYTTSSSSVSQVSDTPVSYTTSSSSVSQVSDTSVPSTSSRSS
VSQVSDTPVPSTSSRSSVSQTSSSLQPTTTSSQRFTISTHGALSESSSVSQQASEITS
SINATASEYHSIQTTAATQSTTLSFTDANSSSASAPLEVATSTPTPSSKASSLLLTPS
TSSLSQVATNTNVQTSLTTESTTVLEPSTTNSSSTFSLVTSSDNNWWIPTELITQAPE
AASTASSTVGGTQTMTLPHAIAAATQVPEPEGYTLITIGFKKALNYEFVVSEPKSSAQ
IFGYLPEALNTPFKNVFTNITVLQIVPLQDDSLNYLVSVAEVYFPTAEIEELSNLITN
SSSAFYTDGMGTAKSMAAMVDSSIPLTGLLHDSNSNSGGSSDGSSSSNSNSGSSGSGS
NSNSGVSSSSGNSYQDAGTLEYSSKSNSNVSTSSKSKKKIIGLVIGVVVGGCLYILFM
IFAFKYIIRRRIQSQEIIKNPEISSISSSEFGGEKNYNNEKRMSVQESITQSMRIQNW
MDDSYYGHGLTNNDSTPTRHNTSSSIPKISRPIASQNSLGWNEV"
gene complement(<521090..>522076)
/gene="EAT1"
/locus_tag="YGR015C"
/db_xref="GeneID:852898"
mRNA complement(<521090..>522076)
/gene="EAT1"
/locus_tag="YGR015C"
/product="putative hydrolase"
/transcript_id="NM_001181144.1"
/db_xref="GeneID:852898"
CDS complement(521090..522076)
/gene="EAT1"
/locus_tag="YGR015C"
/EC_number="2.3.1.268"
/EC_number="3.1.2.1"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0016453
C-acetyltransferase activity [PMID:28356220]"
/note="Ethanol acetyltransferase; green fluorescent
protein (GFP)-fusion protein localizes to the
mitochondrion"
/codon_start=1
/product="putative hydrolase"
/protein_id="NP_011529.1"
/db_xref="GeneID:852898"
/db_xref="SGD:S000003247"
/translation="MSRLAHNKALPYKIIVDLSFHRTRLPSDVSSLIKFEQRPAIINI
HGLLGSHVMFHSLNKLLSRKLDADIFSVDVRNHGISPKAIPYDYTTLTNDLIYFIETH
IGLERPIYLLGFSMGGKIALLTTLYKNINIRKCISIDLPPYETPELDPMILQNYDLIM
RIIRRDVKILRGSPSWQKKVLELFKSLECNKRKCGGAVALYFANGFLSVKSNNVHQAQ
LHYEQQQHDPYINYSMPLSSMPNLLDEVKKWPDLSNQRDFFQKGTASRKVLFMKGLQS
NFINNDYSLLRYNFPCADVREFNTGHNLLLENPEDSFKCILNFFAEETLDFE"
gene <522261..>522833
/locus_tag="YGR016W"
/db_xref="GeneID:852899"
mRNA <522261..>522833
/locus_tag="YGR016W"
/product="uncharacterized protein"
/transcript_id="NM_001181145.1"
/db_xref="GeneID:852899"
CDS 522261..522833
/locus_tag="YGR016W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011530.1"
/db_xref="GeneID:852899"
/db_xref="SGD:S000003248"
/translation="MSRLRRFNRKILSLSSDYTHDGESDQEDVSILPLDTEEQEELIQ
KFETNAHITNKLYINLLSILYLLYGGLLMILVRKSRGYIKLALLAGANSLICSCITLR
YDIVNDYLLFKKFKLRVSNFSINIINIILLVLMAWISFNHVVEDKKTVLCLQVPMFLF
WVAVLVKRWARNIEDEIADLRCLKYKYKNA"
gene complement(<523246..>523353)
/locus_tag="YGR016C-A"
/db_xref="GeneID:91000623"
mRNA complement(<523246..>523353)
/locus_tag="YGR016C-A"
/product="uncharacterized protein"
/transcript_id="NM_001431139.1"
/db_xref="GeneID:91000623"
CDS complement(523246..523353)
/locus_tag="YGR016C-A"
/note="Endoplasmic reticulum hypothetical protein;
deletion confers resistance to fluconazole and to osmotic
stress"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001418068.1"
/db_xref="GeneID:91000623"
/db_xref="SGD:S000350096"
/translation="MAVYSYYPIDMVLLAHLIGIIYLIIILGTLVMLFS"
gene <523787..>524680
/locus_tag="YGR017W"
/db_xref="GeneID:852900"
mRNA <523787..>524680
/locus_tag="YGR017W"
/product="uncharacterized protein"
/transcript_id="NM_001181146.1"
/db_xref="GeneID:852900"
CDS 523787..524680
/locus_tag="YGR017W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="Pyridoxamine-phosphate oxidase-related protein
(PNPO-RP); deletion causes significant increase in
4.5.6-NADH3"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011531.1"
/db_xref="GeneID:852900"
/db_xref="SGD:S000003249"
/translation="MSHQMAPWIPMFIQSCKNNTEPFVSFQFATVDELTNKPRCRTVV
FRDFLFHDKRTNVLTFNTDMRSSKITESFITPNSNNSSDSKRCETPFFEACFYFPETW
EQYRFSGQCFTISKQFKKIPAEIVTKYDIFSPRFSETNDDSTDEEIDTPINDDDDDDK
NNDADNNDINEDNKLIESIENDEHHEDEDDYYPQPQEWEAELLRQWSSLSRHTKSLYR
KPAPGQKLTSETSKQLDKLHRGVDGAKEDAGLENFGIVCLCVDSVDFLNLKEGRGGER
WIFQKTDGKDEDLWEEQEVCP"
gene complement(<524365..>524694)
/locus_tag="YGR018C"
/db_xref="GeneID:852901"
mRNA complement(<524365..>524694)
/locus_tag="YGR018C"
/product="uncharacterized protein"
/transcript_id="NM_001270747.1"
/db_xref="GeneID:852901"
CDS complement(524365..524694)
/locus_tag="YGR018C"
/note="hypothetical protein; mRNA identified as translated
by ribosome profiling data; partially overlaps the
uncharacterized ORF YGR017W"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001257676.1"
/db_xref="GeneID:852901"
/db_xref="SGD:S000003250"
/translation="MIDISHGHTSCSSHRSSSLPSVFWKIHLSPPLPSFKFKKSTEST
QRQTIPKFSSPASSLAPSTPLWSLSSCLLVSEVSFWPGAGFLYNDLVCLDREDHCLSN
SASHSCG"
gene <525229..>526644
/gene="UGA1"
/locus_tag="YGR019W"
/db_xref="GeneID:852902"
mRNA <525229..>526644
/gene="UGA1"
/locus_tag="YGR019W"
/product="4-aminobutyrate transaminase"
/transcript_id="NM_001181148.3"
/db_xref="GeneID:852902"
CDS 525229..526644
/gene="UGA1"
/locus_tag="YGR019W"
/EC_number="2.6.1.19"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:23740823]"
/experiment="EXISTENCE:mutant phenotype:GO:0009450
gamma-aminobutyric acid catabolic process [PMID:3888627]"
/experiment="EXISTENCE:mutant phenotype:GO:0034386
4-aminobutyrate:2-oxoglutarate transaminase activity
[PMID:3888627]"
/note="Gamma-aminobutyrate (GABA) transaminase; also known
as 4-aminobutyrate aminotransferase; involved in the
4-aminobutyrate and glutamate degradation pathways;
required for normal oxidative stress tolerance and
nitrogen utilization; protein abundance increases in
response to DNA replication stress"
/codon_start=1
/product="4-aminobutyrate transaminase"
/protein_id="NP_011533.3"
/db_xref="GeneID:852902"
/db_xref="SGD:S000003251"
/translation="MSICEQYYPEEPTKPTVKTESIPGPESQKQLKELGEVFDTRPAY
FLADYEKSLGNYITDVDGNTYLDLYAQISSIALGYNNPALIKAAQSPEMIRALVDRPA
LGNFPSKDLDKILKQILKSAPKGQDHVWSGLSGADANELAFKAAFIYYRAKQRGYDAD
FSEKENLSVMDNDAPGAPHLAVLSFKRAFHGRLFASGSTTCSKPIHKLDFPAFHWPHA
EYPSYQYPLDENSDANRKEDDHCLAIVEELIKTWSIPVAALIIEPIQSEGGDNHASKY
FLQKLRDITLKYNVVYIIDEVQTGVGATGKLWCHEYADIQPPVDLVTFSKKFQSAGYF
FHDPKFIPNKPYRQFNTWCGEPARMIIAGAIGQEISDKKLTEQCSRVGDYLFKKLEGL
QKKYPENFQNLRGKGRGTFIAWDLPTGEKRDLLLKKLKLNGCNVGGCAVHAVRLRPSL
TFEEKHADIFIEALAKSVNEL"
gene complement(<526973..>527329)
/gene="VMA7"
/locus_tag="YGR020C"
/db_xref="GeneID:852903"
mRNA complement(<526973..>527329)
/gene="VMA7"
/locus_tag="YGR020C"
/product="H(+)-transporting V1 sector ATPase subunit F"
/transcript_id="NM_001181149.1"
/db_xref="GeneID:852903"
CDS complement(526973..527329)
/gene="VMA7"
/locus_tag="YGR020C"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762]"
/note="Subunit F of the V1 peripheral membrane domain of
V-ATPase; part of the electrogenic proton pump found
throughout the endomembrane system; required for the V1
domain to assemble onto the vacuolar membrane; the V1
peripheral membrane domain of vacuolar H+-ATPase
(V-ATPase) has eight subunits"
/codon_start=1
/product="H(+)-transporting V1 sector ATPase subunit F"
/protein_id="NP_011534.1"
/db_xref="GeneID:852903"
/db_xref="SGD:S000003252"
/translation="MAEKRTLIAVIADEDTTTGLLLAGIGQITPETQEKNFFVYQEGK
TTKEEITDKFNHFTEERDDIAILLINQHIAENIRARVDSFTNAFPAILEIPSKDHPYD
PEKDSVLKRVRKLFGE"
gene <527632..>528504
/gene="DPC29"
/locus_tag="YGR021W"
/db_xref="GeneID:852904"
mRNA <527632..>528504
/gene="DPC29"
/locus_tag="YGR021W"
/product="post-initiation translation factor DPC29"
/transcript_id="NM_001181150.1"
/db_xref="GeneID:852904"
CDS 527632..528504
/gene="DPC29"
/locus_tag="YGR021W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095|PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0099617 matrix side
of mitochondrial inner membrane [PMID:36620885]"
/experiment="EXISTENCE:genetic interaction:GO:0032543
mitochondrial translation [PMID:36620885]"
/experiment="EXISTENCE:mutant phenotype:GO:0032543
mitochondrial translation [PMID:36620885]"
/note="General post-initiation mitochondrial translation
factor; peripherally associated with the matrix side of
the mitochondrial inner membrane; interacts with
mitoribosomal proteins in actively translating cells;
human ortholog TACO1 complements the yeast null mutant"
/codon_start=1
/product="post-initiation translation factor DPC29"
/protein_id="NP_011535.1"
/db_xref="GeneID:852904"
/db_xref="SGD:S000003253"
/translation="MLVRNRYLGELLKNSRSFSVLNSSVRSGHNKWSTIKHGKAKNDA
ERNKINNKFANQIAMSVKLGNGITDPSMNIRLATSIELANKNNVSKKVIENAIRKASG
SSASGKDSNASELCVYEGMGPGGVAIVVEALTDNKNRTIGLIRSAFNKANGSMTPTLF
FFDKKGYVTMVPPKMLDTEDKVLESVLEIQGIEDIAPVQEDAEDLECDTETETTGQTY
EAVMEPADTNKVAALLKERGFHIRDLGIGYNAKPDMEVFVQGDETLEKLQKLTTALED
IDEVTSLYTNASNA"
gene <529264..>530919
/gene="MTL1"
/locus_tag="YGR023W"
/db_xref="GeneID:852905"
mRNA <529264..>530919
/gene="MTL1"
/locus_tag="YGR023W"
/product="Mtl1p"
/transcript_id="NM_001181152.1"
/db_xref="GeneID:852905"
CDS 529264..530919
/gene="MTL1"
/locus_tag="YGR023W"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:27337501]"
/experiment="EXISTENCE:genetic interaction:GO:0034599
cellular response to oxidative stress [PMID:20388713]"
/experiment="EXISTENCE:genetic interaction:GO:0042149
cellular response to glucose starvation [PMID:20388713]"
/experiment="EXISTENCE:mutant phenotype:GO:0031505
fungal-type cell wall organization [PMID:10330137]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress
[PMID:15637049|PMID:20388713]"
/experiment="EXISTENCE:mutant phenotype:GO:0042149
cellular response to glucose starvation [PMID:20388713]"
/note="MID-type transmembrane mechanosensor of the CWI
pathway; involved in cell wall integrity (CWI) signaling
and the stress response during glucose starvation and
oxidative stress; structurally and functionally similar to
Mid2p; MTL1 has a paralog, MID2, that arose from the whole
genome duplication; functionally analogous to mammalian
integrins"
/codon_start=1
/product="Mtl1p"
/protein_id="NP_011537.1"
/db_xref="GeneID:852905"
/db_xref="SGD:S000003255"
/translation="MASCNPTRKKSSASSLSMWRTILMALTTLPLSVLSQELVPANST
TSSTAPSITSLSAVESFTSSTDATSSASLSTPSIASVSFTSFPQSSSLLTLSSTLSSE
LSSSSMQVSSSSTSSSSSEVTSSSSSSSISPSSSSSTIISSSSSLPTFTVASTSSTVA
SSTLSTSSSLVISTSSSTFTFSSESSSSLISSSISTSVSTSSVYVPSSSTSSPPSSSS
ELTSSSYSSSSSSSTLFSYSSSFSSSSSSSSSSSSSSSSSSSSSSSYFTLSTSSSSSI
YSSSSYPSFSSSSSSNPTSSITSTSASSSITPASEYSNLAKTITSIIEGQTILSNYYT
TITYSPTASASSGKNSHHSGLSKKNRNIIIGCVVGIGAPLILILLILIYMFCVQPKKT
DFIDSDGKIVTAYRSNIFTKIWYFLLGKKIGETERFSSDSPIGSNNIQNFGDIDPEDI
LNNDNPYTPKHTNVEGYDDDDDDDANDENLSSNFHNRGIDDQYSPTKSASYSMSNSNS
QDYNDADEVMHDENIHRVYDDSEASIDENYYTKPNNGLNITNY"
gene 531610..531681
/locus_tag="YNCG0021W"
/db_xref="GeneID:852907"
tRNA 531610..531681
/locus_tag="YNCG0021W"
/product="tRNA-Asp"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:852907"
/db_xref="SGD:S000006532"
gene complement(<531883..>532596)
/gene="THG1"
/locus_tag="YGR024C"
/db_xref="GeneID:852908"
mRNA complement(<531883..>532596)
/gene="THG1"
/locus_tag="YGR024C"
/product="tRNA guanylyltransferase"
/transcript_id="NM_001181153.3"
/db_xref="GeneID:852908"
CDS complement(531883..532596)
/gene="THG1"
/locus_tag="YGR024C"
/EC_number="2.7.7.79"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006400 tRNA
modification [PMID:14633974]"
/experiment="EXISTENCE:direct assay:GO:0008193 tRNA
guanylyltransferase activity [PMID:14633974]"
/note="tRNAHis guanylyltransferase; adds a guanosine
residue to the 5' end of tRNAH is after transcription and
RNase P cleavage; can also catalyze reverse (3'-5')
polymerization with certain substrates in a
template-dependent reaction; couples nuclear division and
migration to cell budding and cytokinesis; essential
enzyme conserved among eukaryotes"
/codon_start=1
/product="tRNA guanylyltransferase"
/protein_id="NP_011538.3"
/db_xref="GeneID:852908"
/db_xref="SGD:S000003256"
/translation="MANSKFGYVRQFETHDVILPQCYIVVRIDGKKFHEFSKFYEFAK
PNDENALKLMNACAKNLVLKYKNDIILAFGESDEYSFILKSSTTLFNRRKDKLATLFG
SFFTSNYVALWAKFFPEKPLNIKHLPYFDSRCVAYPNLQTIKDYLSWRYVDTHINNLY
NTTFWQLIIKCGLTPQESEKKLCGTFSNEKQEILFSECGINYNNEPEMFKKGSLVTRK
GEILHINVIAQIDELFEGY"
gene <532639..>532941
/locus_tag="YGR025W"
/db_xref="GeneID:852909"
mRNA <532639..>532941
/locus_tag="YGR025W"
/product="uncharacterized protein"
/transcript_id="NM_001348839.1"
/db_xref="GeneID:852909"
CDS 532639..532941
/locus_tag="YGR025W"
/note="hypothetical protein; conserved across S.
cerevisiae strains"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335780.1"
/db_xref="GeneID:852909"
/db_xref="SGD:S000003257"
/translation="MTGYEPFKFFSIRMSSINSPSVIFKTIKTFNPGKTEEYRAKKAY
VQDKGYLRQKRPCPFYYRIPTCTASKYSIGCASSKKLTYTRTKFKFNLVTQRENSL"
gene <532985..>533821
/locus_tag="YGR026W"
/db_xref="GeneID:852910"
mRNA <532985..>533821
/locus_tag="YGR026W"
/product="uncharacterized protein"
/transcript_id="NM_001181155.3"
/db_xref="GeneID:852910"
CDS 532985..533821
/locus_tag="YGR026W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to the cell periphery"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011540.3"
/db_xref="GeneID:852910"
/db_xref="SGD:S000003258"
/translation="MAKTIKVIRKKDPKKKNLSDPLAKQKLVWKIGHVLTLVFGLLFS
ITYFYHVLIFFKYRSWKWLFLRVNKNYSFIQSKRWYMKLLSWSPQVMYRLSLIGVFMS
ESVTMQQNWVGLNPTWNDLLSSENFHTLLIACLWFFGGGKSFYKILPYMILSYLHLTK
MNYELNANKEEKIPLTPKDRKMLHLLAYSELLVILALTLDTILFKTGTSGFMLVIYVG
IYWLRLNFSPYAQVAVLELLVKFEKYVPKKYRDKWQVIKNFIYMKMKEHEKRTEEVAR
YA"
gene complement(<534132..>534458)
/gene="RPS25A"
/locus_tag="YGR027C"
/gene_synonym="RPS31A"
/db_xref="GeneID:852911"
mRNA complement(<534132..>534458)
/gene="RPS25A"
/locus_tag="YGR027C"
/gene_synonym="RPS31A"
/product="40S ribosomal protein eS25 RPS25A"
/transcript_id="NM_001181156.1"
/db_xref="GeneID:852911"
CDS complement(534132..534458)
/gene="RPS25A"
/locus_tag="YGR027C"
/gene_synonym="RPS31A"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:6814480]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:6814480]"
/experiment="EXISTENCE:direct assay:GO:0022627 cytosolic
small ribosomal subunit [PMID:6814480]"
/experiment="EXISTENCE:mutant phenotype:GO:0006451
translational readthrough [PMID:39806023]"
/note="Protein component of the small (40S) ribosomal
subunit; homologous to mammalian ribosomal protein S25, no
bacterial homolog; RPS25A has a paralog, RPS25B, that
arose from the whole genome duplication"
/codon_start=1
/product="40S ribosomal protein eS25 RPS25A"
/protein_id="NP_011541.1"
/db_xref="GeneID:852911"
/db_xref="SGD:S000003259"
/translation="MPPKQQLSKAAKAAAALAGGKKSKKKWSKKSMKDRAQHAVILDQ
EKYDRILKEVPTYRYVSVSVLVDRLKIGGSLARIALRHLEKEGIIKPISKHSKQAIYT
RATASE"
repeat_region 535211..535542
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006965"
repeat_region complement(535607..535759)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006952"
mobile_element 535760..541685
/note="YGRWTy1-1; Ty1 element, LTR retrotransposon of the
Copia (Pseudoviridae) group; contains co-transcribed genes
TYA Gag and TYB Pol, encoding proteins involved in
structure and function of virus-like particles, flanked by
two direct repeats"
/mobile_element_type="retrotransposon:YGRWTy1-1"
/db_xref="SGD:S000006982"
repeat_region 535760..536097
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006966"
gene <536057..>541325
/locus_tag="YGR027W-B"
/db_xref="GeneID:852913"
mRNA <536057..>541325
/locus_tag="YGR027W-B"
/product="gag-pol fusion protein"
/transcript_id="NM_001184432.2"
/db_xref="GeneID:852913"
CDS join(536057..537361,537363..541325)
/locus_tag="YGR027W-B"
/EC_number="2.7.7.7"
/EC_number="2.7.7.49"
/EC_number="3.1.26.4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9448009]"
/ribosomal_slippage
/note="Retrotransposon TYA Gag and TYB Pol genes;
transcribed/translated as one unit; polyprotein is
processed to make a nucleocapsid-like protein (Gag),
reverse transcriptase (RT), protease (PR), and integrase
(IN); similar to retroviral genes"
/codon_start=1
/product="gag-pol fusion protein"
/protein_id="NP_058158.1"
/db_xref="GeneID:852913"
/db_xref="SGD:S000007406"
/translation="MESQQLSQHSHISHGSACASVTSKEVHTNQDPLDVSASKTEECE
KASTKANSQQTTTPASSAVPENPHHASPQTAQSHSPQNGPYPQQCMMTQNQANPSGWS
FYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVRQITDDELT
FLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQ
YNGSTPADAFETKVTNIIDRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNM
TVAELFLDIHAIYEEQQGSRNSKPNYRRNLSDEKNDSRSYTNTTKPKVIARNPQKTNN
SKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLGQELTESTVNHTNHS
DDELPGHLLLDSGASRTLIRSAHHIHSASSNPDINVVDAQKRNIPINAIGDLQFHFQD
NTKTSIKVLHTPNIAYDLLSLNELAAVDITACFTKNVLERSDGTVLAPIVQYGDFYWV
SKRYLLPSNISVPTINNVHTSESTRKYPYPFIHRMLAHANAQTIRYSLKNNTITYFNE
SDVDWSSAIDYQCPDCLIGKSTKHRHIKGSRLKYQNSYEPFQYLHTDIFGPVHNLPKS
APSYFISFTDETTKFRWVYPLHDRREDSILDVFTTILAFIKNQFQASVLVIQMDRGSE
YTNRTLHKFLEKNGITPCYTTTADSRAHGVAERLNRTLLDDCRTQLQCSGLPNHLWFS
AIEFSTIVRNSLASPKSKKSARQHAGLAGLDISTLLPFGQPVIVNDHNPNSKIHPRGI
PGYALHPSRNSYGYIIYLPSLKKTVDTTNYVILQGKESRLDQFNYDALTFDEDLNRLT
ASYHSFIASNEIQESNDLNIESDHDFQSDIELHPEQPRNVLSKAVSPTDSTPPSTHTE
DSKRVSKTNIRAPREVDPNISESNILPSKKRSSTPQISNIESTGSGGMHKLNVPLLAP
MSQSNTHESSHASKSKDFRHSDSYSENETNHTNVPISSTGGTNNKTVPQISDQETEKR
IIHRSPSIDASPPENNSSHNIVPIKTPTTVSEQNTEESIIADLPLPDLPPESPTEFPD
PFKELPPINSRQTNSSLGGIGDSNAYTTINSKKRSLEDNETEIKVSRDTWNTKNMRSL
EPPRSKKRIHLIAAVKAVKSIKPIRTTLRYDEAITYNKDIKEKEKYIEAYHKEVNQLL
KMKTWDTDEYYDRKEIDPKRVINSMFIFNKKRDGTHKARFVARGDIQHPDTYDSGMQS
NTVHHYALMTSLSLALDNNYYITQLDISSAYLYADIKEELYIRPPPHLGMNDKLIRLK
KSLYGLKQSGANWYETIKSYLIQQCGMEEVRGWSCVFKNSQVTICLFVDDMVLFSKNL
NSNKRIIDKLKMQYDTKIINLGESDEEIQYDILGLEIKYQRGKYMKLGMENSLTEKIP
KLNVPLNPKGRKLSAPGQPGLYIDQQELELEEDDYKMKVHEMQKLIGLASYVGYKFRF
DLLYYINTLAQHILFPSKQVLDMTYELIQFIWNTRDKQLIWHKSKPVKPTNKLVVISD
ASYGNQPYYKSQIGNIYLLNGKVIGGKSTKASLTCTSTTEAEIHAISESVPLLNNLSY
LIQELDKKPITKGLLTDSKSTISIIISNNEEKFRNRFFGTKAMRLRDEVSGNHLHVCY
IETKKNIADVMTKPLPIKTFKLLTNKWIH"
gene <536057..>537379
/locus_tag="YGR027W-A"
/db_xref="GeneID:852912"
mRNA <536057..>537379
/locus_tag="YGR027W-A"
/product="gag protein"
/transcript_id="NM_001184431.1"
/db_xref="GeneID:852912"
CDS 536057..537379
/locus_tag="YGR027W-A"
/note="Retrotransposon TYA Gag gene co-transcribed with
TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is
a nucleocapsid protein that is the structural constituent
of virus-like particles (VLPs); similar to retroviral Gag"
/codon_start=1
/product="gag protein"
/protein_id="NP_058159.1"
/db_xref="GeneID:852912"
/db_xref="SGD:S000007405"
/translation="MESQQLSQHSHISHGSACASVTSKEVHTNQDPLDVSASKTEECE
KASTKANSQQTTTPASSAVPENPHHASPQTAQSHSPQNGPYPQQCMMTQNQANPSGWS
FYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVRQITDDELT
FLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQ
YNGSTPADAFETKVTNIIDRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNM
TVAELFLDIHAIYEEQQGSRNSKPNYRRNLSDEKNDSRSYTNTTKPKVIARNPQKTNN
SKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLRPGTY"
repeat_region 541348..541685
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006967"
gene 541850..541921
/locus_tag="YNCG0022W"
/db_xref="GeneID:852914"
tRNA 541850..541921
/locus_tag="YNCG0022W"
/product="tRNA-Glu"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE
analysis; thiolation of uridine at wobble position (34)
requires Ncs6p; target of K. lactis zymocin"
/db_xref="GeneID:852914"
/db_xref="SGD:S000006555"
gene <542203..>543291
/gene="MSP1"
/locus_tag="YGR028W"
/gene_synonym="YTA4"
/db_xref="GeneID:852915"
mRNA <542203..>543291
/gene="MSP1"
/locus_tag="YGR028W"
/gene_synonym="YTA4"
/product="protein-degrading AAA family ATPase MSP1"
/transcript_id="NM_001181157.3"
/db_xref="GeneID:852915"
CDS 542203..543291
/gene="MSP1"
/locus_tag="YGR028W"
/gene_synonym="YTA4"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:8226973|PMID:16407407]"
/experiment="EXISTENCE:direct assay:GO:0005778 peroxisomal
membrane [PMID:24821790]"
/experiment="EXISTENCE:direct assay:GO:0045047 protein
targeting to ER [PMID:31445887]"
/experiment="EXISTENCE:mutant phenotype:GO:0006626 protein
targeting to mitochondrion [PMID:8226973]"
/note="Highly-conserved N-terminally anchored AAA-ATPase;
distributed in the mitochondrial outer membrane and
peroxisomes; involved in mitochondrial protein sorting;
ATP-driven extractase that pulls mislocalized
tail-anchored proteins into the cytosol, passing them to
the GET pathway for transfer to the ER membrane for
degradation, ensuring fidelity of organelle-specific
localization of tail-anchored proteins; contains an
N-terminal transmembrane domain and C-terminal cytoplasmic
ATPase domain"
/codon_start=1
/product="protein-degrading AAA family ATPase MSP1"
/protein_id="NP_011542.3"
/db_xref="GeneID:852915"
/db_xref="SGD:S000003260"
/translation="MSRKFDLKTITDLSVLVGTGISLYYLVSRLLNDVESGPLSGKSR
ESKAKQSLQWEKLVKRSPALAEVTLDAYERTILSSIVTPDEINITFQDIGGLDPLISD
LHESVIYPLMMPEVYSNSPLLQAPSGVLLYGPPGCGKTMLAKALAKESGANFISIRMS
SIMDKWYGESNKIVDAMFSLANKLQPCIIFIDEIDSFLRERSSTDHEVTATLKAEFMT
LWDGLLNNGRVMIIGATNRINDIDDAFLRRLPKRFLVSLPGSDQRYKILSVLLKDTKL
DEDEFDLQLIADNTKGFSGSDLKELCREAALDAAKEYIKQKRQLIDSGTIDVNDTSSL
KIRPLKTKDFTKKLRMDATSTLSSQPLD"
gene <543553..>544205
/gene="ERV1"
/locus_tag="YGR029W"
/db_xref="GeneID:852916"
mRNA join(<543553..543638,543722..>544205)
/gene="ERV1"
/locus_tag="YGR029W"
/product="flavin-linked sulfhydryl oxidase"
/transcript_id="NM_001181158.3"
/db_xref="GeneID:852916"
CDS join(543553..543638,543722..544205)
/gene="ERV1"
/locus_tag="YGR029W"
/EC_number="1.8.3.2"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005758
mitochondrial intermembrane space [PMID:11493598]"
/experiment="EXISTENCE:direct assay:GO:0006879
intracellular iron ion homeostasis [PMID:11493598]"
/experiment="EXISTENCE:direct assay:GO:0016971
flavin-dependent sulfhydryl oxidase activity
[PMID:10899311]"
/experiment="EXISTENCE:mutant phenotype:GO:0016972 thiol
oxidase activity [PMID:15989955]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress [PMID:15989955]"
/experiment="EXISTENCE:mutant phenotype:GO:0045041 protein
import into mitochondrial intermembrane space
[PMID:16181637]"
/experiment="EXISTENCE:mutant phenotype:GO:0160203
mitochondrial disulfide relay system [PMID:15989955]"
/note="Flavin-linked sulfhydryl oxidase of the
mitochondrial IMS; N-terminus is an intrinsically
disordered domain that in the cytosol helps target Erv1p
to mitochondria, and in the intermembrane space oxidizes
Mia40p as part of a disulfide relay system that promotes
intermembrane space retention of imported proteins;
functional ortholog of human GFER (ALR); human GFER
carrying N-terminal 21 amino acids of Erv1p functionally
complements the lethality of the erv1 null mutation"
/codon_start=1
/product="flavin-linked sulfhydryl oxidase"
/protein_id="NP_011543.4"
/db_xref="GeneID:852916"
/db_xref="SGD:S000003261"
/translation="MKAIDKMTDNPPQEGLSGRKIIYDEDGKPCRSCNTLLDFQYVTG
KISNGLKNLSSNGKLAGTGALTGEASELMPGSRTYRKVDPPDVEQLGRSSWTLLHSVA
ASYPAQPTDQQKGEMKQFLNIFSHIYPCNWCAKDFEKYIRENAPQVESREELGRWMCE
AHNKVNKKLRKPKFDCNFWEKRWKDGWDE"
gene complement(544577..544648)
/locus_tag="YNCG0023C"
/db_xref="GeneID:852917"
tRNA complement(544577..544648)
/locus_tag="YNCG0023C"
/product="tRNA-Asp"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:852917"
/db_xref="SGD:S000006533"
gene 545370..545566
/gene="SNR46"
/locus_tag="YNCG0024W"
/db_xref="GeneID:9164897"
ncRNA 545370..545566
/ncRNA_class="snoRNA"
/gene="SNR46"
/locus_tag="YNCG0024W"
/product="SNR46"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:9160748]"
/experiment="EXISTENCE:mutant phenotype:GO:0030559 rRNA
pseudouridylation guide activity [PMID:9160748]"
/experiment="EXISTENCE:mutant phenotype:GO:0031118 rRNA
pseudouridine synthesis [PMID:9160748]"
/experiment="EXISTENCE:physical interaction:GO:0031429 box
H/ACA snoRNP complex [PMID:15923376]"
/note="H/ACA box small nucleolar RNA (snoRNA); guides
pseudouridylation of large subunit (LSU) rRNA at position
U2865"
/transcript_id="NR_132182.1"
/db_xref="GeneID:9164897"
/db_xref="SGD:S000006506"
gene complement(<545681..>546157)
/gene="POP6"
/locus_tag="YGR030C"
/db_xref="GeneID:852918"
mRNA complement(<545681..>546157)
/gene="POP6"
/locus_tag="YGR030C"
/product="ribonuclease P/MRP protein subunit POP6"
/transcript_id="NM_001181159.1"
/db_xref="GeneID:852918"
CDS complement(545681..546157)
/gene="POP6"
/locus_tag="YGR030C"
/EC_number="3.1.26.5"
/experiment="EXISTENCE:direct assay:GO:0000172
ribonuclease MRP complex [PMID:9620854]"
/experiment="EXISTENCE:direct assay:GO:0000294
nuclear-transcribed mRNA catabolic process, RNase
MRP-dependent [PMID:14729943]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:26135751]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005655 nucleolar
ribonuclease P complex [PMID:9620854]"
/experiment="EXISTENCE:direct assay:GO:0005697 telomerase
holoenzyme complex [PMID:27156450]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0034965 intronic
box C/D snoRNA processing [PMID:18713869]"
/experiment="EXISTENCE:direct assay:GO:0042134 rRNA
primary transcript binding [PMID:17881380]"
/experiment="EXISTENCE:mutant phenotype:GO:0000171
ribonuclease MRP activity [PMID:9620854]"
/experiment="EXISTENCE:mutant phenotype:GO:0004526
ribonuclease P activity [PMID:9620854]"
/experiment="EXISTENCE:mutant phenotype:GO:0006364 rRNA
processing [PMID:9620854]"
/experiment="EXISTENCE:mutant phenotype:GO:0008033 tRNA
processing [PMID:9620854]"
/experiment="EXISTENCE:physical interaction:GO:0000172
ribonuclease MRP complex [PMID:17881380]"
/note="Subunit of RNase MRP, nuclear RNase P and
telomerase; forms a soluble heterodimer with Pop7p that
binds P3 domain of RNase MRP and RNase P RNAs; RNase MRP
cleaves pre-rRNA, nuclear RNase P cleaves tRNA precursors
to generate mature 5' ends and facilitates turnover of
nuclear RNAs, while telomerase replenishes telomeric DNA;
relocalizes to the cytosol in response to hypoxia"
/codon_start=1
/product="ribonuclease P/MRP protein subunit POP6"
/protein_id="NP_011544.1"
/db_xref="GeneID:852918"
/db_xref="SGD:S000003262"
/translation="MINGVYYNEISRDLDISSSTQCLRFLKETVIPSLANNGNNSTSI
QYHGISKNDNIKKSVNKLDKQINMADRSLGLQQVVCIFSYGPHIQKMLSILEIFKKGY
IKNNKKIYQWNKLTSFDIKREGRNELQEERLKVPILVTLVSDSEIIDLNLHSFTKQ"
gene <546441..>547469
/gene="IMO32"
/locus_tag="YGR031W"
/db_xref="GeneID:852919"
mRNA <546441..>547469
/gene="IMO32"
/locus_tag="YGR031W"
/product="Imo32p"
/transcript_id="NM_001181160.1"
/db_xref="GeneID:852919"
CDS 546441..547469
/gene="IMO32"
/locus_tag="YGR031W"
/experiment="EXISTENCE:direct assay:GO:0004806
triacylglycerol lipase activity [PMID:28465236]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:16823961|PMID:14562095|PMID:24769239|PMID:21525245]"
/experiment="EXISTENCE:direct assay:GO:0006629 lipid
metabolic process [PMID:28465236]"
/experiment="EXISTENCE:mutant phenotype:GO:0004806
triacylglycerol lipase activity [PMID:28465236]"
/note="Conserved mitochondrial hypothetical protein;
processed by both mitochondrial processing peptidase and
mitochondrial octapeptidyl aminopeptidase; gene contains
the nested antisense gene NAG1"
/codon_start=1
/product="Imo32p"
/protein_id="NP_011545.1"
/db_xref="GeneID:852919"
/db_xref="SGD:S000003263"
/translation="MMILGKAGILAQYGTIYVRQNTIRNNLSSCIFKQSLCAFHSLAK
VLQQKQVPLDLSYDIIKRDAVKTGDEGKPRPPIIILHGLFGNKLNNRSIGRNLNKKLG
RDVYLLDLRNHGSSPHSSVHNYEVMSEDVKHFITKHELNTNGGPIIIGHSMGGKVAMM
LVLKNPQLCSMLVCIENAPVSLRPNAEFVEYIKALMEIVNDKGKTIRTLKQADEHLAE
RIGGNELVRRFLLTALKKVKMDNSSSVSSYTFEERIPLATLKDAIVKGEIAAWPLDPA
RERWTRPALFIRATQSHYVVDEYLPIIGAFFPRFETRDIDAGHWVNAEKPGECAESIV
DFVERHED"
gene complement(<546809..>547300)
/gene="NAG1"
/locus_tag="YGR031C-A"
/db_xref="GeneID:8656591"
mRNA complement(<546809..>547300)
/gene="NAG1"
/locus_tag="YGR031C-A"
/product="Nag1p"
/transcript_id="NM_001184658.1"
/db_xref="GeneID:8656591"
CDS complement(546809..547300)
/gene="NAG1"
/locus_tag="YGR031C-A"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:18310357]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0042546 cell
wall biogenesis [PMID:18310357]"
/note="Protein involved in yeast cell wall biogenesis;
localizes to the cell periphery; production of Nag1p is
dependent upon the presence of Slt2p and Rlm1p; gene is
nested within and antisense to IMO32"
/codon_start=1
/product="Nag1p"
/protein_id="NP_001027023.1"
/db_xref="GeneID:8656591"
/db_xref="SGD:S000028636"
/translation="MNSAGRVHRSRAGSRGHAAISPLTMASFSVARGIRSSNVYDDTD
DELSILTFFSAVRRNRLTSSLPPILSARCSSACFSVRIVLPLSLTISISALMYSTNSA
LGRKLTGAFSIQTNIEQSCGFFRTSIMATLPPIECPIIIGPPLVFNSCFVIKCFTSSD
MTS"
gene <548264..>553951
/gene="GSC2"
/locus_tag="YGR032W"
/gene_synonym="FKS2"
/db_xref="GeneID:852920"
mRNA <548264..>553951
/gene="GSC2"
/locus_tag="YGR032W"
/gene_synonym="FKS2"
/product="1,3-beta-glucan synthase GSC2"
/transcript_id="NM_001181161.3"
/db_xref="GeneID:852920"
CDS 548264..553951
/gene="GSC2"
/locus_tag="YGR032W"
/gene_synonym="FKS2"
/EC_number="2.4.1.34"
/experiment="EXISTENCE:direct assay:GO:0000148
1,3-beta-D-glucan synthase complex [PMID:7983071]"
/experiment="EXISTENCE:direct assay:GO:0003843
1,3-beta-D-glucan synthase activity
[PMID:7649185|PMID:7565718]"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:15755916]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0006075
(1->3)-beta-D-glucan biosynthetic process [PMID:7565718]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0030476
ascospore wall assembly [PMID:16116083]"
/experiment="EXISTENCE:mutant phenotype:GO:0006075
(1->3)-beta-D-glucan biosynthetic process [PMID:7565718]"
/experiment="EXISTENCE:mutant phenotype:GO:0030476
ascospore wall assembly [PMID:16116083]"
/note="Catalytic subunit of 1,3-beta-glucan synthase;
involved in formation of the inner layer of the spore
wall; activity positively regulated by Rho1p and
negatively by Smk1p; GSC2 has a paralog, FKS1, that arose
from the whole genome duplication"
/codon_start=1
/product="1,3-beta-glucan synthase GSC2"
/protein_id="NP_011546.3"
/db_xref="GeneID:852920"
/db_xref="SGD:S000003264"
/translation="MSYNDPNLNGQYYSNGDGTGDGNYPTYQVTQDQSAYDEYGQPIY
TQNQLDDGYYDPNEQYVDGTQFPQGQDPSQDQGPYNNDASYYNQPPNMMNPSSQDGEN
FSDFSSYGPPSGTYPNDQYTPSQMSYPDQDGSSGASTPYGNGVVNGNGQYYDPNAIEM
ALPNDPYPAWTADPQSPLPIEQIEDIFIDLTNKFGFQRDSMRNMFDHFMTLLDSRSSR
MSPEQALLSLHADYIGGDTANYKKWYFAAQLDMDDEIGFRNMKLGKLSRKARKAKKKN
KKAMQEASPEDTEETLNQIEGDNSLEAADFRWKSKMNQLSPFEMVRQIALFLLCWGEA
NQVRFTPECLCFIYKCASDYLDSAQCQQRPDPLPEGDFLNRVITPLYRFIRSQVYEIV
DGRYVKSEKDHNKVIGYDDVNQLFWYPEGIAKIVMEDGTRLIDLPAEERYLKLGEIPW
DDVFFKTYKETRSWLHLVTNFNRIWIMHISVYWMYCAYNAPTFYTHNYQQLVDNQPLA
AYKWATAALGGTVASLIQVAATLCEWSFVPRKWAGAQHLSRRFWFLCVIMGINLGPVI
FVFAYDKDTVYSTAAHVVGAVMFFVAVATLVFFSVMPLGGLFTSYMKKSTRSYVASQT
FTASFAPLHGLDRWMSYLVWVTVFAAKYAESYFFLILSLRDPIRILSTTSMRCTGEYW
WGNKICKVQPKIVLGLMIATDFILFFLDTYLWYIVVNTVFSVGKSFYLGISILTPWRN
IFTRLPKRIYSKILATTDMEIKYKPKVLISQIWNAIIISMYREHLLAIDHVQKLLYHQ
VPSEIEGKRTLRAPTFFVSQDDNNFETEFFPRDSEAERRISFFAQSLSTPIPEPLPVD
NMPTFTVLTPHYAERILLSLREIIREDDQFSRVTLLEYLKQLHPVEWDCFVKDTKILA
EETAAYENNEDEPEKEDALKSQIDDLPFYCIGFKSAAPEYTLRTRIWASLRSQTLYRT
ISGFMNYSRAIKLLYRVENPEIVQMFGGNADGLERELEKMARRKFKFLVSMQRLAKFK
PHELENAEFLLRAYPDLQIAYLDEEPPLNEGEEPRIYSALIDGHCEILENGRRRPKFR
VQLSGNPILGDGKSDNQNHALIFYRGEYIQLIDANQDNYLEECLKIRSVLAEFEELGI
EQIHPYTPGLKYEDQSTNHPVAIVGAREYIFSENSGVLGDVAAGKEQTFGTLFARTLA
QIGGKLHYGHPDFINATFMTTRGGVSKAQKGLHLNEDIYAGMNAVLRGGRIKHCEYYQ
CGKGRDLGFGTILNFTTKIGAGMGEQMLSREYYYLGTQLPIDRFLTFYYAHPGFHLNN
LFIQLSLQMFMLTLVNLHALAHESILCVYDRDKPITDVLYPIGCYNFHPAIDWVRRYT
LSIFIVFWIAFVPIVVQELIERGLWKATQRFFRHILSLSPMFEVFAGQIYSSALLSDI
AVGGARYISTGRGFATSRIPFSILYSRFAGSAIYMGSRSMLMLLFGTVAHWQAPLLWF
WASLSALIFAPFIFNPHQFAWEDFFLDYRDYIRWLSRGNNKYHRNSWIGYVRMSRSRV
TGFKRKLVGDESEKSAGDASRAHRTNLIMAEIIPCAIYAAGCFIAFTFINAQTGVKTT
DEDRVNSTLRIIICTLAPIVIDIGVLFFCMGLSCCSGPLLGMCCKKTGSVMAGIAHGI
AVVVHIVFFIVMWVLEGFSFVRMLIGVVTCIQCQRLIFHCMTVLLLTREFKNDHANTA
FWTGKWYSTGLGYMAWTQPTRELTAKVIELSEFAADFVLGHVILIFQLPVICIPKIDK
FHSIMLFWLKPSRQIRPPIYSLKQARLRKRMVRRYCSLYFLVLIIFAGCIVGPAVASA
HVPKDLGSGLTGTFHNLVQPRNVSNNDTGSQMSTYKSHYYTHTPSLKTWSTIK"
gene complement(<554248..>554967)
/gene="TIM21"
/locus_tag="YGR033C"
/db_xref="GeneID:852921"
mRNA complement(<554248..>554967)
/gene="TIM21"
/locus_tag="YGR033C"
/product="Tim21p"
/transcript_id="NM_001181162.3"
/db_xref="GeneID:852921"
CDS complement(554248..554967)
/gene="TIM21"
/locus_tag="YGR033C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:24769239|PMID:14576278|PMID:16823961|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005744 TIM23
mitochondrial import inner membrane translocase complex
[PMID:15797382|PMID:15878866]"
/experiment="EXISTENCE:mutant phenotype:GO:0030150 protein
import into mitochondrial matrix [PMID:15797382]"
/note="Nonessential component of the TIM23 complex;
interacts with the Translocase of the Outer Mitochondrial
membrane (TOM complex) and with respiratory enzymes; may
regulate the Translocase of the Inner Mitochondrial
membrane (TIM23 complex) activity"
/codon_start=1
/product="Tim21p"
/protein_id="NP_011547.3"
/db_xref="GeneID:852921"
/db_xref="SGD:S000003265"
/translation="MSSSLPRSLLRLGHRKPLFPRYNTFVNSSVITHTSLLRTRLYSN
GTGATSGKKDDKTRNKPKPLWPQVKSASTFTFSGILVIGAVGISAIVIYLILSELFSP
SGDTQLFNRAVSMVEKNKDIRSLLQCDDGITGKERLKAYGELITNDKWTRNRPIVSTK
KLDKEGRTHHYMRFHVESKKKIALVHLEAKESKQNYQPDFINMYVDVPGEKRYYLIKP
KLHPVSNSKGFLGIRWGPRKD"
gene <555812..>556672
/gene="RPL26B"
/locus_tag="YGR034W"
/db_xref="GeneID:852922"
mRNA join(<555812..555830,556308..>556672)
/gene="RPL26B"
/locus_tag="YGR034W"
/product="60S ribosomal protein uL24 RPL26B"
/transcript_id="NM_001181163.1"
/db_xref="GeneID:852922"
CDS join(555812..555830,556308..556672)
/gene="RPL26B"
/locus_tag="YGR034W"
/experiment="EXISTENCE:direct assay:GO:0002181 cytoplasmic
translation [PMID:18782943]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:6337137]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:18782943]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:18782943]"
/note="Ribosomal 60S subunit protein L26B; binds to 5.8S
rRNA; non-essential even when paralog is also deleted;
deletion has minimal affections on ribosome biosynthesis;
homologous to mammalian ribosomal protein L26 and
bacterial L24; RPL26B has a paralog, RPL26A, that arose
from the whole genome duplication"
/codon_start=1
/product="60S ribosomal protein uL24 RPL26B"
/protein_id="NP_011548.2"
/db_xref="GeneID:852922"
/db_xref="SGD:S000003266"
/translation="MAKQSLDVSSDRRKARKAYFTAPSSERRVLLSAPLSKELRAQYG
IKALPIRRDDEVLVVRGSKKGQEGKISSVYRLKFAVQVDKVTKEKVNGASVPINLHPS
KLVITKLHLDKDRKALIQRKGGKLE"
gene complement(<557072..>557422)
/locus_tag="YGR035C"
/db_xref="GeneID:852923"
mRNA complement(<557072..>557422)
/locus_tag="YGR035C"
/product="uncharacterized protein"
/transcript_id="NM_001181164.1"
/db_xref="GeneID:852923"
CDS complement(557072..557422)
/locus_tag="YGR035C"
/note="hypothetical protein, potential Cdc28p substrate;
transcription is activated by paralogous transcription
factors Yrm1p and Yrr1p along with genes involved in
multidrug resistance; YGR035C has a paralog, YLR346C, that
arose from the whole genome duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011549.1"
/db_xref="GeneID:852923"
/db_xref="SGD:S000003267"
/translation="MLLTPAKTTRTEDSANSTDDSSKSSNSFMRAIVSSLMVKPITSL
TNTVTCRQSSHHNSSPSKITRYDLIKAAAENDLKRSKSQGREKSRRNSNRRNNEEIFV
ANTASEIQRTKSSI"
gene <557565..>557786
/locus_tag="YGR035W-A"
/db_xref="GeneID:1466457"
mRNA <557565..>557786
/locus_tag="YGR035W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184648.1"
/db_xref="GeneID:1466457"
CDS 557565..557786
/locus_tag="YGR035W-A"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878077.1"
/db_xref="GeneID:1466457"
/db_xref="SGD:S000028827"
/translation="MLLYLYILYIALKTVSHLFFCNPCFRNLSVGDMLNPRLSLSFFT
NHLLCPEAPLIIRGKGSYSAVGNFFSRKK"
gene complement(<558149..>558868)
/gene="CAX4"
/locus_tag="YGR036C"
/gene_synonym="CWH8"
/db_xref="GeneID:852924"
mRNA complement(<558149..>558868)
/gene="CAX4"
/locus_tag="YGR036C"
/gene_synonym="CWH8"
/product="dolichyldiphosphatase"
/transcript_id="NM_001181165.3"
/db_xref="GeneID:852924"
CDS complement(558149..558868)
/gene="CAX4"
/locus_tag="YGR036C"
/gene_synonym="CWH8"
/EC_number="3.6.1.43"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:11504728]"
/experiment="EXISTENCE:direct assay:GO:0047874
dolichyldiphosphatase activity [PMID:11504728]"
/experiment="EXISTENCE:mutant phenotype:GO:0006487 protein
N-linked glycosylation [PMID:10024662]"
/experiment="EXISTENCE:mutant phenotype:GO:0008610 lipid
biosynthetic process [PMID:11504728]"
/note="Dolichyl pyrophosphate (Dol-P-P) phosphatase;
cleaves the anhydride linkage in Dol-P-P; involved in
synthesis of Dol-P-P-linked oligosaccharide intermediate
required for protein N-glycosylation; has a luminally
oriented active site in the ER; mutation affects vesicular
transport, membrane and cell wall biogenesis and lipid
homeostasis"
/codon_start=1
/product="dolichyldiphosphatase"
/protein_id="NP_011550.3"
/db_xref="GeneID:852924"
/db_xref="SGD:S000003268"
/translation="MNSTAAAINPNPNVIPFDDTYILYDSHDFLSFLSAYFSLMPILV
LAFYLSWFIITRELEACIVAFGQLMNEIFNNVIKNIIKQPRPVSFGASFQNDTIRSGY
GMPSAHSQFMGFCFTYNSLKIYTSWKNLNFLEKCIFSGALALLSFCVCFSRVYLHYHN
LDQVIVGFSVGALTGSLYFFIVGIIRELGLINWFLKLRIVRLFYMTDSYNLAPLTLKE
NYEAYWKRINQRSFNDKSKRD"
gene complement(<559731..>559994)
/gene="ACB1"
/locus_tag="YGR037C"
/db_xref="GeneID:852925"
mRNA complement(<559731..>559994)
/gene="ACB1"
/locus_tag="YGR037C"
/product="long-chain fatty acid transporter ACB1"
/transcript_id="NM_001181166.3"
/db_xref="GeneID:852925"
CDS complement(559731..559994)
/gene="ACB1"
/locus_tag="YGR037C"
/experiment="EXISTENCE:direct assay:GO:0005576
extracellular region [PMID:20156967]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0005576
extracellular region [PMID:20156967]"
/experiment="EXISTENCE:mutant phenotype:GO:0036042
long-chain fatty acyl-CoA binding [PMID:11294913]"
/experiment="EXISTENCE:mutant phenotype:GO:0042761 very
long-chain fatty acid biosynthetic process
[PMID:11294913]"
/note="Acyl-CoA-binding protein; transports newly
synthesized acyl-CoA esters from fatty acid synthetase
(Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to
starvation-induced, Grh1p-mediated unconventional
secretion; protein abundance increases in response to DNA
replication stress"
/codon_start=1
/product="long-chain fatty acid transporter ACB1"
/protein_id="NP_011551.3"
/db_xref="GeneID:852925"
/db_xref="SGD:S000003269"
/translation="MVSQLFEEKAKAVNELPTKPSTDELLELYALYKQATVGDNDKEK
PGIFNMKDRYKWEAWENLKGKSQEDAEKEYIALVDQLIAKYSS"
gene <560682..>561350
/gene="ORM1"
/locus_tag="YGR038W"
/db_xref="GeneID:852926"
mRNA <560682..>561350
/gene="ORM1"
/locus_tag="YGR038W"
/product="sphingolipid homeostasis protein ORM1"
/transcript_id="NM_001181167.1"
/db_xref="GeneID:852926"
CDS 560682..561350
/gene="ORM1"
/locus_tag="YGR038W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:20182505|PMID:14562095|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0017059 serine
palmitoyltransferase complex [PMID:20182505]"
/experiment="EXISTENCE:genetic interaction:GO:0090155
negative regulation of sphingolipid biosynthetic process
[PMID:20182505]"
/experiment="EXISTENCE:mutant phenotype:GO:0006986
response to unfolded protein [PMID:12093374]"
/experiment="EXISTENCE:mutant phenotype:GO:0090155
negative regulation of sphingolipid biosynthetic process
[PMID:20182505]"
/experiment="EXISTENCE:physical interaction:GO:0090155
negative regulation of sphingolipid biosynthetic process
[PMID:20182505]"
/note="Protein that mediates sphingolipid homeostasis;
evolutionarily conserved, required for resistance to
agents that induce unfolded protein response; Orm1p and
Orm2p together control membrane biogenesis by coordinating
lipid homeostasis with protein quality control; ORM1 has a
paralog, ORM2, that arose from the whole genome
duplication"
/codon_start=1
/product="sphingolipid homeostasis protein ORM1"
/protein_id="NP_011552.1"
/db_xref="GeneID:852926"
/db_xref="SGD:S000003270"
/translation="MTELDYQGTAEAASTSYSRNQTDLKPFPSAGSASSSIKTTEPVK
DHRRRRSSSIISHVEPETFEDENDQQLLPNMNATWVDQRGAWIIHVVIIILLKLFYNL
FPGVTTEWSWTLTNMTYVIGSYVMFHLIKGTPFDFNGGAYDNLTMWEQIDDETLYTPS
RKFLISVPIALFLVSTHYAHYDLKLFSWNCFLTTFGAVVPKLPVTHRLRISIPGITGR
AQIS"
gene complement(561662..561743)
/locus_tag="YNCG0025C"
/db_xref="GeneID:852927"
tRNA complement(561662..561743)
/locus_tag="YNCG0025C"
/product="tRNA-Ser"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0030371 translation
repressor activity [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043555 regulation
of translation in response to stress [PMID:27609601]"
/note="Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:852927"
/db_xref="SGD:S000006725"
mobile_element complement(561843..567760)
/note="YGRCTy1-2; Ty1 element, LTR retrotransposon of the
Copia (Pseudoviridae) group; contains co-transcribed genes
TYA Gag and TYB Pol, encoding proteins involved in
structure and function of virus-like particles, flanked by
two direct repeats"
/mobile_element_type="retrotransposon:YGRCTy1-2"
/db_xref="SGD:S000006962"
repeat_region complement(561843..562176)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006953"
gene complement(<562199..>567467)
/locus_tag="YGR038C-B"
/db_xref="GeneID:852928"
mRNA complement(<562199..>567467)
/locus_tag="YGR038C-B"
/product="gag-pol fusion protein"
/transcript_id="NM_001184434.2"
/db_xref="GeneID:852928"
CDS complement(join(562199..566161,566163..567467))
/locus_tag="YGR038C-B"
/EC_number="2.7.7.7"
/EC_number="2.7.7.49"
/EC_number="3.1.26.4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9448009]"
/ribosomal_slippage
/note="Retrotransposon TYA Gag and TYB Pol genes;
transcribed/translated as one unit; polyprotein is
processed to make a nucleocapsid-like protein (Gag),
reverse transcriptase (RT), protease (PR), and integrase
(IN); similar to retroviral genes"
/codon_start=1
/product="gag-pol fusion protein"
/protein_id="NP_058160.1"
/db_xref="GeneID:852928"
/db_xref="SGD:S000007408"
/translation="MESQQLSQHSHISHGSACASVTSKEVHTNQDPLDVSASKIQEYD
KASTKANSQQTTTPASSAVPENPHHASPQPASVPPPQNGPYPQQCMMTQNQANPSGWS
FYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVRQITDDELT
FLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQ
YNGSTPADAFETKVTNIIDRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNM
TVAELFLDIHAIYEEQQGSRNSKPNYRRNPSDEKNDSRSYTNTTKPKVIARNPQKTNN
SKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLGQELTESTVNHTNHS
DDELPGHLLLDSGASRTLIRSAHHIHSASSNPGINVVDAQKRNIPINAIGDLQFHFQD
NTKTSIKVLHTPNIAYDLLSLNELAAVDITACFTKNVLERSDGTVLAPIVKYGDFYWV
SKKYLLPSNISVPTINNVHTSESTRKYPYPFIHRMLAHANAQTIRYSLKNNTITYFNE
SDVDWSSAIDYQCPDCLIGKSTKHRHIKGSRLKYQNSYEPFQYLHTDIFGPVHNLPNS
APSYFISFTDETTKFRWVYPLHDRREDSILDVFTTILAFIKNQFQASVLVIQMDRGSE
YTNRTLHKFLEKNGITPCYTTTADSRAHGVAERLNRTLLDDCRTQLQCSGLPNHLWFS
AIEFSTIVRNSLASPKSKKSARQHAGLAGLDISTLLPFGQPVIVNDHNPNSKIHPRGI
PGYALHPSRNSYGYIIYLPSLKKTVDTTNYVILQGKESRLDQFNYDALTFDEDLNRLT
ASYHSFIASNEIQESNDLNIESDHDFQSDIELHPEQPRNVLSKAVSPTDSTPPSTHTE
DSKRVSKTNIRAPREVDPNISESNILPSKKRSSTPQISNIESTGSGGMHKLNVPLLAP
MSQSNTHESSHASKSKDFRHSDSYSENETNHTNVPISSTGGTNNKTVPQISDQETEKR
IIHRSPSIDASPPENNSSHNIVPIKTPTTVSEQNTEESIIADLPLPDLPPESPTEFPD
PFKELPPINSHQTNSSLGGIGDSNAYTTINSKKRSLEDNETEIKVSRDTWNTKNMRSL
EPPRSKKRIHLIAAVKAVKSIKPIRTTLRYDEAITYNKDIKEKEKYIEAYHKEVNQLL
KMNTWDTDKYYDRKEIDPKRVINSMFIFNRKRDGTHKARFVARGDIQHPDTYDSGMQS
NTVHHYALMTSLSLALDNNYYITQLDISSAYLYADIKEELYIRPPPHLGMNDKLIRLK
KSLYGLKQSGANWYETIKSYLIKQCGMEEVRGWSCVFKNSQVTICLFVDDMILFSKDL
NANKKIITTLKKQYDTKIINLGESDNEIQYDILGLEIKYQRGKYMKLGMENSLTEKIP
KLNVPLNPKGRKLSAPGQPGLYIDQDELEIDEDEYKEKVHEMQKLIGLASYVGYKFRF
DLLYYINTLAQHILFPSRQVLDMTYELIQFMWDTRDKQLIWHKNKPTEPDNKLVAISD
ASYGNQPYYKSQIGNIFLLNGKVIGGKSTKASLTCTSTTEAEIHAVSEAIPLLNNLSH
LVQELNKKPIIKGLLTDSRSTISIIKSTNEEKFRNRFFGTKAMRLRDEVSGNNLYVYY
IETKKNIADVMTKPLPIKTFKLLTNKWIH"
gene complement(<566145..>567467)
/locus_tag="YGR038C-A"
/db_xref="GeneID:852929"
mRNA complement(<566145..>567467)
/locus_tag="YGR038C-A"
/product="gag protein"
/transcript_id="NM_001184433.1"
/db_xref="GeneID:852929"
CDS complement(566145..567467)
/locus_tag="YGR038C-A"
/note="Retrotransposon TYA Gag gene co-transcribed with
TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is
a nucleocapsid protein that is the structural constituent
of virus-like particles (VLPs); similar to retroviral Gag"
/codon_start=1
/product="gag protein"
/protein_id="NP_058161.1"
/db_xref="GeneID:852929"
/db_xref="SGD:S000007407"
/translation="MESQQLSQHSHISHGSACASVTSKEVHTNQDPLDVSASKIQEYD
KASTKANSQQTTTPASSAVPENPHHASPQPASVPPPQNGPYPQQCMMTQNQANPSGWS
FYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVRQITDDELT
FLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQ
YNGSTPADAFETKVTNIIDRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNM
TVAELFLDIHAIYEEQQGSRNSKPNYRRNPSDEKNDSRSYTNTTKPKVIARNPQKTNN
SKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLRPGTY"
repeat_region complement(567427..567760)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006954"
rep_origin 568490..568737
/note="ARS721; Autonomously Replicating Sequence"
/db_xref="SGD:S000118461"
mobile_element complement(568740..574700)
/note="YGRCTy2-1; Ty2 element, LTR retrotransposon of the
Copia (Pseudoviridae) group; contains genes TYA Gag and
TYB Pol, encoding proteins involved in structure and
function of virus-like particles, flanked by two direct
repeats; mutated in S288C"
/mobile_element_type="retrotransposon:YGRCTy2-1"
/db_xref="SGD:S000006964"
repeat_region complement(568740..569072)
/note="Ty2 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006955"
repeat_region complement(574369..574700)
/note="Ty2 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006956"
rep_origin 574622..574915
/note="ARS722; Autonomously Replicating Sequence"
/db_xref="SGD:S000118462"
gene <574888..>575199
/locus_tag="YGR039W"
/db_xref="GeneID:852930"
mRNA <574888..>575199
/locus_tag="YGR039W"
/product="uncharacterized protein"
/transcript_id="NM_001348840.1"
/db_xref="GeneID:852930"
CDS 574888..575199
/locus_tag="YGR039W"
/note="hypothetical protein; conserved among S. cerevisiae
strains; YGR039W is not an essential gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335781.1"
/db_xref="GeneID:852930"
/db_xref="SGD:S000003271"
/translation="MLFFTGLVILSYLLGGKKDWLLIMYEASDYLEFLNRLSSMLFKF
ELFEHLSSTFLIFSHSKRAVKLLEIYRSRHISIYNVFLLRHPKYFQSVDIALYLTRSQ
T"
gene <575398..>576504
/gene="KSS1"
/locus_tag="YGR040W"
/db_xref="GeneID:852931"
mRNA <575398..>576504
/gene="KSS1"
/locus_tag="YGR040W"
/product="mitogen-activated serine/threonine-protein
kinase KSS1"
/transcript_id="NM_001181169.3"
/db_xref="GeneID:852931"
CDS 575398..576504
/gene="KSS1"
/locus_tag="YGR040W"
/EC_number="2.7.11.24"
/experiment="EXISTENCE:direct assay:GO:0000749 response to
pheromone triggering conjugation with cellular fusion
[PMID:8918885|PMID:8668180]"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0004707 MAP kinase
activity [PMID:8918885|PMID:8668180]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:18417610|PMID:7579701]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:27400980]"
/experiment="EXISTENCE:genetic interaction:GO:0010973
positive regulation of division septum assembly
[PMID:27400980]"
/experiment="EXISTENCE:mutant phenotype:GO:0000750
pheromone-dependent signal transduction involved in
conjugation with cellular fusion [PMID:11583629]"
/experiment="EXISTENCE:mutant phenotype:GO:0001402 signal
transduction involved in filamentous growth
[PMID:9393860]"
/experiment="EXISTENCE:mutant phenotype:GO:0001403
invasive growth in response to glucose limitation
[PMID:8001818]"
/note="Mitogen-activated protein kinase (MAPK); involved
in signal transduction pathways that control filamentous
growth and pheromone response; regulates septum assembly,
and may directly phosphorylate Bni4p; the KSS1 gene is
nonfunctional in some S288C-related strains and functional
in W303a strains"
/codon_start=1
/product="mitogen-activated serine/threonine-protein
kinase KSS1"
/protein_id="NP_011554.3"
/db_xref="GeneID:852931"
/db_xref="SGD:S000003272"
/translation="MARTITFDIPSQYKLVDLIGEGAYGTVCSAIHKPSGIKVAIKKI
QPFSKKLFVTRTIREIKLLRYFHEHENIISILDKVRPVSIDKLNAVYLVEELMETDLQ
KVINNQNSGFSTLSDDHVQYFTYQILRALKSIHSAQVIHRDIKPSNLLLNSNCDLKVC
DFGLARCLASSSDSRETLVGFMTEYVATRWYRAPEIMLTFQEYTTAMDIWSCGCILAE
MVSGKPLFPGRDYHHQLWLILEVLGTPSFEDFNQIKSKRAKEYIANLPMRPPLPWETV
WSKTDLNPDMIDLLDKMLQFNPDKRISAAEALRHPYLAMYHDPSDEPEYPPLNLDDEF
WKLDNKIMRPEEEEEVPIEMLKDMLYDELMKTME"
rep_origin 575484..576346
/gene="KSS1"
/locus_tag="YGR040W"
/note="ARS723; Putative replication origin; identified in
multiple array studies, not yet confirmed by plasmid-based
assay"
/db_xref="SGD:S000130166"
gene <577487..>579130
/gene="BUD9"
/locus_tag="YGR041W"
/db_xref="GeneID:852932"
mRNA <577487..>579130
/gene="BUD9"
/locus_tag="YGR041W"
/product="Bud9p"
/transcript_id="NM_001181170.3"
/db_xref="GeneID:852932"
CDS 577487..579130
/gene="BUD9"
/locus_tag="YGR041W"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:11514631|PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0000282
cellular bud site selection [PMID:8657162]"
/experiment="EXISTENCE:mutant phenotype:GO:0005886 plasma
membrane [PMID:17581861]"
/note="Protein involved in bud-site selection; mutant has
increased aneuploidy tolerance; diploid mutants display a
unipolar budding pattern instead of the wild-type bipolar
pattern, and bud at the distal pole; BUD9 has a paralog,
BUD8, that arose from the whole genome duplication"
/codon_start=1
/product="Bud9p"
/protein_id="NP_011555.3"
/db_xref="GeneID:852932"
/db_xref="SGD:S000003273"
/translation="MTKITRDVSITTENSKSTSGSATASSASLPENDHPIFHQPRARI
RSGSLFIEGSDSFPSSEVKSYNVYIDDSKYSEILKGDTNSSSTDGKQVFEDARDDNFH
QESHRDLEDSILDLVRRDPEVAAFPLPPPNSNERNRNSSNGSSAETNLNGHSSSGTIS
TSVLLNMGSAEKHAGTTRGDHMESSSMKSFEKLGTRPSSLFYPPPEGTAPYQGPRATV
SGNKSTRQTQGTYSFPSMRYGVDLVSPVEGAVDVAKSRVPNSTLNGTFPDKAFIPHEF
QIPKKAWNRIPANKSTSLKTPRNHSLLIDILKPFEAADLANDQRSSSAVLKNTVHSNG
QYNPTNETSGTRMQDQRQKNTNEIDLEKIPNPQVPLGIAMDTMRSPNQLHEKEYESNI
EAGLASGVGKGDNSIKQHQYKKIPQEIDRDQQLSFQMETMPIQRIDSSSIRSFDSRIY
GFSEIYSIPRVITTLCICLFVPPLFFFFSINGNNGVSNYRLMRMIMNYEHRIGLLKGF
EWDIDVQWFRTLCFVLGCIEMLAIFASIGIGFGVGIIRE"
gene <579476..>580291
/gene="MTE1"
/locus_tag="YGR042W"
/db_xref="GeneID:852933"
mRNA <579476..>580291
/gene="MTE1"
/locus_tag="YGR042W"
/product="Mte1p"
/transcript_id="NM_001181171.1"
/db_xref="GeneID:852933"
CDS 579476..580291
/gene="MTE1"
/locus_tag="YGR042W"
/experiment="EXISTENCE:direct assay:GO:0000781 chromosome,
telomeric region [PMID:26966248]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008047 enzyme
activator activity [PMID:26966248]"
/experiment="EXISTENCE:direct assay:GO:0035861 site of
double-strand break [PMID:26966248]"
/experiment="EXISTENCE:mutant phenotype:GO:0032205
negative regulation of telomere maintenance
[PMID:26966248]"
/note="hypothetical protein; involved in maintenance of
proper telomere length; green fluorescent protein
(GFP)-fusion protein localizes to both the cytoplasm and
the nucleus; forms nuclear foci upon DNA replication
stress"
/codon_start=1
/product="Mte1p"
/protein_id="NP_011556.1"
/db_xref="GeneID:852933"
/db_xref="SGD:S000003274"
/translation="MLSHIVEYECQYTDQLYKKRKIWHDGRLKYFQLNNRFMLYTEKD
NVLLASEFKINSKELKAILNPEGFDIEEHRIFSQFLVIISNIIEEYDRDIQVAATHVR
AYPSNLSVQKQRPLISDNAPSLNHISTAREVHSNIKVTTPNRKQTEDNATKGGFNISK
LTLKVNKPFKKPKRILSTNVVNESNRPSIRSQKIQEVTPQLHETNTSTKVQTAGKVAL
NNDNIAQGNYATITEEAKVRDGSDRKKDMANLSKSGKRRVGGIRRIVHEPLGI"
gene complement(<580435..>581436)
/gene="NQM1"
/locus_tag="YGR043C"
/db_xref="GeneID:852934"
mRNA complement(<580435..>581436)
/gene="NQM1"
/locus_tag="YGR043C"
/product="sedoheptulose-7-phosphate:D-glyceraldehyde-3-
phosphate transaldolase NQM1"
/transcript_id="NM_001181172.1"
/db_xref="GeneID:852934"
CDS complement(580435..581436)
/gene="NQM1"
/locus_tag="YGR043C"
/EC_number="2.2.1.2"
/experiment="EXISTENCE:direct assay:GO:0004801
transaldolase activity [PMID:18831051]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress [PMID:25887987]"
/note="Transaldolase of unknown function; transcription is
repressed by Mot1p and induced by alpha-factor and during
diauxic shift; NQM1 has a paralog, TAL1, that arose from
the whole genome duplication"
/codon_start=1
/product="sedoheptulose-7-phosphate:D-glyceraldehyde-3-
phosphate transaldolase NQM1"
/protein_id="NP_011557.1"
/db_xref="GeneID:852934"
/db_xref="SGD:S000003275"
/translation="MSEPSEKKQKVATSSLEQLKKAGTHVVADSGDFEAISKYEPQDS
TTNPSLILAASKLEKYARFIDAAVEYGRKHGKTDHEKIENAMDKILVEFGTQILKVVP
GRVSTEVDARLSFDKKATVKKALHIIKLYKDAGVPKERVLIKIASTWEGIQAARELEV
KHGIHCNMTLLFSFTQAVACAEANVTLISPFVGRIMDFYKALSGKDYTAETDPGVLSV
KKIYSYYKRHGYATEVMAASFRNLDELKALAGIDNMTLPLNLLEQLYESTDPIENKLN
SESAKEEGVEKVSFINDEPHFRYVLNEDQMATEKLSDGIRKFSADIEALYKLVEEKM"
rep_origin 581436..582213
/note="ARS724; Putative replication origin; identified in
multiple array studies, not yet confirmed by plasmid-based
assay"
/db_xref="SGD:S000130167"
gene complement(<582990..>583892)
/gene="RME1"
/locus_tag="YGR044C"
/gene_synonym="CSP1"
/db_xref="GeneID:852935"
mRNA complement(<582990..>583892)
/gene="RME1"
/locus_tag="YGR044C"
/gene_synonym="CSP1"
/product="Rme1p"
/transcript_id="NM_001181173.1"
/db_xref="GeneID:852935"
CDS complement(582990..583892)
/gene="RME1"
/locus_tag="YGR044C"
/gene_synonym="CSP1"
/experiment="EXISTENCE:direct assay:GO:0000082 G1/S
transition of mitotic cell cycle [PMID:11713667]"
/experiment="EXISTENCE:direct assay:GO:0000122 negative
regulation of transcription by RNA polymerase II
[PMID:9580682]"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:9580682]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11713667|PMID:9580682|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0045944 positive
regulation of transcription by RNA polymerase II
[PMID:12006664|PMID:14668363|PMID:11713667]"
/experiment="EXISTENCE:direct assay:GO:2000219 positive
regulation of invasive growth in response to glucose
limitation [PMID:14668363]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:8339935]"
/experiment="EXISTENCE:mutant phenotype:GO:0000981
DNA-binding transcription factor activity, RNA polymerase
II-specific [PMID:8339935]"
/experiment="EXISTENCE:mutant phenotype:GO:0045835
negative regulation of meiotic nuclear division
[PMID:3513021]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:12006664]"
/experiment="EXISTENCE:mutant phenotype:GO:0051321 meiotic
cell cycle [PMID:8339935]"
/note="Zinc finger protein involved in control of meiosis;
prevents meiosis by repressing IME1 expression and
promotes mitosis by activating CLN2 expression; directly
repressed by a1-alpha2 regulator; mediates cell type
control of sporulation; relocalizes from nucleus to
cytoplasm upon DNA replication stress"
/codon_start=1
/product="Rme1p"
/protein_id="NP_011558.1"
/db_xref="GeneID:852935"
/db_xref="SGD:S000003276"
/translation="MSPCYGQNSAIAKGSWNREVLQEVQPIYHWHDFGQNMKEYSASP
LEGDSSLPSSLPSSTEDCLLLSLENTITVIAGNQRQAYDSTSSTEEGTAPQLRPDEIA
DSTHCITSLVDPEFRDLINYGRQKGANPVFIESNTTEQSHSQCILGYPQKSHVAQLYH
DPKVLSTISEGQTKRGSYHCSHCSEKFATLVEFAAHLDEFNLERPCKCPIEQCPWKIL
GFQQATGLRRHCASQHIGELDIEMEKSLNLKVEKYPGLNCPFPICQKTFRRKDAYKRH
VAMVHNNADSRFNKRLKKILNNTK"
gene complement(<583934..>584296)
/locus_tag="YGR045C"
/db_xref="GeneID:852936"
mRNA complement(<583934..>584296)
/locus_tag="YGR045C"
/product="uncharacterized protein"
/transcript_id="NM_001348841.1"
/db_xref="GeneID:852936"
CDS complement(583934..584296)
/locus_tag="YGR045C"
/note="hypothetical protein; conserved across S.
cerevisiae strains"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335782.1"
/db_xref="GeneID:852936"
/db_xref="SGD:S000003277"
/translation="MSQITSKGRRILDKKIRTFPVGFTSRKVAGHVLNISPYFLLAFS
YAENKGQSAFEEIKGSNVIDMSCVICFNFSCHLFVVIFISRSTETIPTTKLLLSKYIF
YCVNALELTLFLSYKSYS"
gene <584895..>586052
/gene="TAM41"
/locus_tag="YGR046W"
/gene_synonym="MMP37"
/db_xref="GeneID:852937"
mRNA <584895..>586052
/gene="TAM41"
/locus_tag="YGR046W"
/gene_synonym="MMP37"
/product="putative phosphatidate cytidylyltransferase"
/transcript_id="NM_001181175.3"
/db_xref="GeneID:852937"
CDS 584895..586052
/gene="TAM41"
/locus_tag="YGR046W"
/gene_synonym="MMP37"
/EC_number="2.7.7.41"
/experiment="EXISTENCE:direct assay:GO:0004605
phosphatidate cytidylyltransferase activity
[PMID:23623749]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14690591|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:16943180|PMID:16790493]"
/experiment="EXISTENCE:genetic interaction:GO:0032049
cardiolipin biosynthetic process [PMID:20485265]"
/experiment="EXISTENCE:mutant phenotype:GO:0004605
phosphatidate cytidylyltransferase activity
[PMID:23623749]"
/experiment="EXISTENCE:mutant phenotype:GO:0032049
cardiolipin biosynthetic process [PMID:19114592]"
/note="Mitochondrial phosphatidate cytidylyltransferase
(CDP-DAG synthase); required for cardiolipin biosynthesis;
viability of null mutant is strain-dependent; mRNA is
targeted to the bud; mutant displays defect in
mitochondrial protein import, likely due to altered
membrane lipid composition"
/codon_start=1
/product="putative phosphatidate cytidylyltransferase"
/protein_id="NP_011560.3"
/db_xref="GeneID:852937"
/db_xref="SGD:S000003278"
/translation="MLRVSENGLRFLLKCHSTNVSMFNRLLSTQIKEGRSSIDDAGII
PDGTINERPNHYIEGITKGSDLDLLEKGIRKTDEMTSNFTNYMYKFHRLPPNYGSNQL
ITIDKELQKELDGVMSSFKAPCRFVFGYGSGVFEQAGYSKSHSKPQIDIILGVTYPSH
FHSINMRQNPQHYSSLKYFGSEFVSKFQQIGAGVYFNPFANINGHDVKYGVVSMETLL
KDIATWNTFYLAGRLQKPVKILKNDLRVQYWNQLNLKAAATLAKHYTLEKNNNKFDEF
QFYKEITALSYAGDIRYKLGGENPDKVNNIVTKNFERFQEYYKPIYKEVVLNDSFYLP
KGFTLKNTQRLLLSRISKSSALQTIKGVFTAGITKSIKYAWAKKLKSMRRS"
gene complement(<586392..>589469)
/gene="TFC4"
/locus_tag="YGR047C"
/gene_synonym="PCF1"
/db_xref="GeneID:852938"
mRNA complement(<586392..>589469)
/gene="TFC4"
/locus_tag="YGR047C"
/gene_synonym="PCF1"
/product="transcription factor TFIIIC subunit TFC4"
/transcript_id="NM_001181176.3"
/db_xref="GeneID:852938"
CDS complement(586392..589469)
/gene="TFC4"
/locus_tag="YGR047C"
/gene_synonym="PCF1"
/experiment="EXISTENCE:direct assay:GO:0000127
transcription factor TFIIIC complex
[PMID:2002052|PMID:2180956|PMID:8387209|PMID:1474578]"
/experiment="EXISTENCE:direct assay:GO:0001002 RNA
polymerase III type 1 promoter sequence-specific DNA
binding [PMID:2649882|PMID:2404611]"
/experiment="EXISTENCE:direct assay:GO:0001003 RNA
polymerase III type 2 promoter sequence-specific DNA
binding [PMID:2404611]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0006383
transcription by RNA polymerase III
[PMID:8464480|PMID:3633923|PMID:2183033]"
/experiment="EXISTENCE:direct assay:GO:0008301 DNA
binding, bending [PMID:2002052]"
/experiment="EXISTENCE:direct assay:GO:0042791 5S class
rRNA transcription by RNA polymerase III [PMID:2183033]"
/experiment="EXISTENCE:mutant phenotype:GO:0006383
transcription by RNA polymerase III [PMID:8264649]"
/note="Subunit of RNA polymerase III transcription
initiation factor complex; one of six subunits of RNA
polymerase III transcription initiation factor complex
(TFIIIC); part of the TauA domain of TFIIIC that binds
BoxA DNA promoter sites of tRNA and similar genes; has TPR
motifs; human homolog is TFIIIC-102"
/codon_start=1
/product="transcription factor TFIIIC subunit TFC4"
/protein_id="NP_011561.3"
/db_xref="GeneID:852938"
/db_xref="SGD:S000003279"
/translation="MAAGKLKKEQQNQSAERESADTGKVNDEDEEHLYGNIDDYKHLI
QDEEYDDEDVPHDLQLSEDEYNSERDSSLLAEFSDYGEISEDDEEDFMNAIREASNFK
VKKKKKNDKGKSYGRQRKERVLDPEVAQLLSQANEAFVRNDLQVAERLFNEVIKKDAR
NFAAYETLGDIYQLQGRLNDCCNSWFLAAHLNASDWEFWKIVAILSADLDHVRQAIYC
FSRVISLNPMEWESIYRRSMLYKKTGQLARALDGFQRLYMYNPYDANILRELAILYVD
YDRIEDSIELYMKVFNANVERREAILAALENALDSSDEESAAEGEDADEKEPLEQDED
RQMFPDINWKKIDAKYKCIPFDWSSLNILAELFLKLAVSEVDGIKTIKKCARWIQRRE
SQTFWDHVPDDSEFDNRRFKNSTFDSLLAAEKEKSYNIPIDIRVRLGLLRLNTDNLVE
ALNHFQCLYDETFSDVADLYFEAATALTRAEKYKEAIDFFTPLLSLEEWRTTDVFKPL
ARCYKEIESYETAKEFYELAIKSEPDDLDIRVSLAEVYYRLNDPETFKHMLVDVVEMR
KHQVDETLHRISNEKSSNDTSDISSKPLLEDSKFRTFRKKKRTPYDAERERIERERRI
TAKVVDKYEKMKKFELNSGLNEAKQASIWINTVSELVDIFSSVKNFFMKSRSRKFVGI
LRRTKKFNTELDFQIERLSKLAEGDSVFEGPLMEERVTLTSATELRGLSYEQWFELFM
ELSLVIAKYQSVEDGLSVVETAQEVNVFFQDPERVKMMKFVKLAIVLQMDDEEELAEN
LRGLLNQFQFNRKVLQVFMYSLCRGPSSLNILSSTIQQKFFLRQLKAFDSCRYNTEVN
GQASITNKEVYNPNKKSSPYLYYIYAVLLYSSRGFLSALQYLTRLEEDIPDDPMVNLL
MGLSHIHRAMQRLTAQRHFQIFHGLRYLYRYHKIRKSLYTDLEKQEADYNLGRAFHLI
GLVSIAIEYYNRVLENYDDGKLKKHAAYNSIIIYQQSGNVELADHLMEKYLSI"
gene <589826..>590911
/gene="UFD1"
/locus_tag="YGR048W"
/db_xref="GeneID:852939"
mRNA <589826..>590911
/gene="UFD1"
/locus_tag="YGR048W"
/product="polyubiquitin-binding protein UFD1"
/transcript_id="NM_001181177.1"
/db_xref="GeneID:852939"
CDS 589826..590911
/gene="UFD1"
/locus_tag="YGR048W"
/experiment="EXISTENCE:direct assay:GO:0000837 Doa10p
ubiquitin ligase complex [PMID:16873066|PMID:16873065]"
/experiment="EXISTENCE:direct assay:GO:0000839 Hrd1p
ubiquitin ligase ERAD-L complex
[PMID:16873066|PMID:16873065]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11733065|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0006274 DNA
replication termination [PMID:31509741]"
/experiment="EXISTENCE:direct assay:GO:0030894 replisome
[PMID:31509741]"
/experiment="EXISTENCE:direct assay:GO:0031593
polyubiquitin modification-dependent protein binding
[PMID:16004872]"
/experiment="EXISTENCE:direct assay:GO:0034098
VCP-NPL4-UFD1 AAA ATPase complex
[PMID:11598205|PMID:11733065]"
/experiment="EXISTENCE:direct assay:GO:0043130 ubiquitin
binding [PMID:16004872]"
/experiment="EXISTENCE:direct assay:GO:1990112 RQC complex
[PMID:23178123]"
/experiment="EXISTENCE:mutant phenotype:GO:0030970
retrograde protein transport, ER to cytosol
[PMID:11740563|PMID:11813000]"
/experiment="EXISTENCE:mutant phenotype:GO:0036503 ERAD
pathway [PMID:11813000|PMID:11739805|PMID:11740563]"
/experiment="EXISTENCE:mutant phenotype:GO:0043161
proteasome-mediated ubiquitin-dependent protein catabolic
process [PMID:20206597]"
/experiment="EXISTENCE:mutant phenotype:GO:0070651
nonfunctional rRNA decay [PMID:22505030]"
/experiment="EXISTENCE:mutant phenotype:GO:0071629
cytoplasm protein quality control by the
ubiquitin-proteasome system [PMID:18812321]"
/experiment="EXISTENCE:mutant phenotype:GO:0072665 protein
localization to vacuole [PMID:29444958]"
/experiment="EXISTENCE:mutant phenotype:GO:0072671
mitochondria-associated ubiquitin-dependent protein
catabolic process [PMID:32118579]"
/experiment="EXISTENCE:mutant phenotype:GO:1900182
positive regulation of protein localization to nucleus
[PMID:11733065|PMID:11598205]"
/experiment="EXISTENCE:mutant phenotype:GO:1990116
ribosome-associated ubiquitin-dependent protein catabolic
process [PMID:23358411]"
/note="Substrate-recruiting cofactor of the
Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding
protein that assists in the dislocation of misfolded, ERAD
substrates that are subsequently delivered to the
proteasome for degradation; involved in regulated
destruction of ER membrane proteins such as HMG-CoA
reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p);
involved in mobilizing membrane bound transcription
factors by regulated Ub/proteasome-dependent processing
(RUP)"
/codon_start=1
/product="polyubiquitin-binding protein UFD1"
/protein_id="NP_011562.1"
/db_xref="GeneID:852939"
/db_xref="SGD:S000003280"
/translation="MFSGFSSFGGGNGFVNMPQTFEEFFRCYPIAMMNDRIRKDDANF
GGKIFLPPSALSKLSMLNIRYPMLFKLTANETGRVTHGGVLEFIAEEGRVYLPQWMME
TLGIQPGSLLQISSTDVPLGQFVKLEPQSVDFLDISDPKAVLENVLRNFSTLTVDDVI
EISYNGKTFKIKILEVKPESSSKSICVIETDLVTDFAPPVGYVEPDYKALKAQQDKEK
KNSFGKGQVLDPSVLGQGSMSTRIDYAGIANSSRNKLSKFVGQGQNISGKAPKAEPKQ
DIKDMKITFDGEPAKLDLPEGQLFFGFPMVLPKEDEESAAGSKSSEQNFQGQGISLRK
SNKRKTKSDHDSSKSKAPKSPEVIEID"
gene <591314..>591877
/gene="SCM4"
/locus_tag="YGR049W"
/db_xref="GeneID:852940"
mRNA <591314..>591877
/gene="SCM4"
/locus_tag="YGR049W"
/product="Scm4p"
/transcript_id="NM_001181178.1"
/db_xref="GeneID:852940"
CDS 591314..591877
/gene="SCM4"
/locus_tag="YGR049W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:14562095|PMID:24769239|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:16407407]"
/note="Mitochondrial outer membrane hypothetical protein;
predicted to have 4 transmembrane segments; import is
mediated by Tom70p and Mim1p; interacts genetically with a
cdc4 mutation; SCM4 has a paralog, ATG33, that arose from
the whole genome duplication"
/codon_start=1
/product="Scm4p"
/protein_id="NP_011563.1"
/db_xref="GeneID:852940"
/db_xref="SGD:S000003281"
/translation="MQVSPAIVKGIAVSSLGLYAGILTSSTVISITTPINVLTQHLKN
VLCTLGCWSTVLGGLATGAFGLSYYLAAPGERPNYLLCGLGVAPLSAAYLYLVSLFNH
KLAPKCTRDQNDLEKQKDEKLPQHHPEVKDGEAACPFSKMNNAKTLKPESERSVKCHS
YMSLHMSIVTGITIFTFGKCILDGFKA"
gene complement(<592086..>592442)
/locus_tag="YGR050C"
/db_xref="GeneID:852941"
mRNA complement(<592086..>592442)
/locus_tag="YGR050C"
/product="uncharacterized protein"
/transcript_id="NM_001270748.1"
/db_xref="GeneID:852941"
CDS complement(592086..592442)
/locus_tag="YGR050C"
/note="hypothetical protein; mRNA identified as translated
by ribosome profiling data"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001257677.1"
/db_xref="GeneID:852941"
/db_xref="SGD:S000003282"
/translation="MKTNLISSFAFRPHVRFGFLDFGYSLKSILDVTSQYHFIPIKLA
YGLCDPLLIVYPDPSFSLAQAAGEKKKVVFPTYEDVPKLLLTFKQIMNTYRKGSLGQR
QRQRVYGRNGNESHST"
gene <593596..>594705
/gene="FMP48"
/locus_tag="YGR052W"
/db_xref="GeneID:852943"
mRNA <593596..>594705
/gene="FMP48"
/locus_tag="YGR052W"
/product="protein kinase FMP48"
/transcript_id="NM_001181181.1"
/db_xref="GeneID:852943"
CDS 593596..594705
/gene="FMP48"
/locus_tag="YGR052W"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0006468 protein
phosphorylation [PMID:16319894]"
/note="hypothetical protein; the authentic, non-tagged
protein is detected in highly purified mitochondria in
high-throughput studies; induced by treatment with
8-methoxypsoralen and UVA irradiation"
/codon_start=1
/product="protein kinase FMP48"
/protein_id="NP_011566.1"
/db_xref="GeneID:852943"
/db_xref="SGD:S000003284"
/translation="MYTKLRSIQSGTFSTVYKAWSTTHNRYVALKITPKYKTSEANMK
NEYDVMKILSSCNPHPNICSMLDFYTDDSYYIMVLEYCECGDLYDFLDIAKSQGSPSS
PSLIQIDMQKIIKQLCSAISFAHSLGIAHRDIKPENILLTINGDIKLADWGHAIQSPK
SNDFQIGTDNYRAPETFSGRVSNSCFKKNFDRSSAPLYNTYQADYWSLGATIFYLMFG
DCLFRVSKSKKVQHLKNFDEFEKDPFAFIYRKYVVPRLSCGYNDEEDLHVSLQHTRQY
IWQDLPDIYDVFHLCKIMVDTLLKVSNAKERSMENFINEVDSAWNKDSSMDSCFSYQN
KIDLFWEQWSVNTETVPAKFQLKNFEKPCLIQDGK"
gene complement(<594986..>595837)
/gene="MCO32"
/locus_tag="YGR053C"
/db_xref="GeneID:852944"
mRNA complement(<594986..>595837)
/gene="MCO32"
/locus_tag="YGR053C"
/product="Mco32p"
/transcript_id="NM_001181182.3"
/db_xref="GeneID:852944"
CDS complement(594986..595837)
/gene="MCO32"
/locus_tag="YGR053C"
/note="hypothetical protein"
/codon_start=1
/product="Mco32p"
/protein_id="NP_011567.3"
/db_xref="GeneID:852944"
/db_xref="SGD:S000003285"
/translation="MLLKCVCRAGIISIKGIFPRWYSNSQKVANLGQITDYLVVKGVP
NLLQKMFKESVLADNVVFRLFPTSHPYIPVLHGKSKYMASLNAMRMIVRKFILGDECR
LHISSVKTLTSTSHDEEVKSALQSYNTITCNDKLVIKWQSCIPEDHCKITKLEINDRL
KEEKRGNGAGGSFSMRSVPVIDYILHPTANNLNQSVISEYLENAAERSMENKVNETQN
SKEDEKKKNDGDGKRSKKKRLSRLIKGTFIFEFNEENSKILVHTIEDVELIHYEKKIA
TRGAFAC"
gene <596693..>598621
/locus_tag="YGR054W"
/db_xref="GeneID:852945"
mRNA <596693..>598621
/locus_tag="YGR054W"
/product="translation initiation factor 2A"
/transcript_id="NM_001181183.4"
/db_xref="GeneID:852945"
CDS 596693..598621
/locus_tag="YGR054W"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0003743 translation
initiation factor activity [PMID:12133843]"
/experiment="EXISTENCE:direct assay:GO:0006413
translational initiation [PMID:12133843]"
/experiment="EXISTENCE:direct assay:GO:0022626 cytosolic
ribosome [PMID:12133843]"
/experiment="EXISTENCE:mutant phenotype:GO:0003743
translation initiation factor activity [PMID:12133843]"
/experiment="EXISTENCE:mutant phenotype:GO:0006413
translational initiation [PMID:12133843]"
/experiment="EXISTENCE:mutant phenotype:GO:0017148
negative regulation of translation [PMID:38011112]"
/note="Eukaryotic initiation factor eIF2A; associates
specifically with both 40S subunits and 80S ribosomes, and
interacts genetically with both eIF5b and eIF4E;
physically interacts with Ssd1p, and associates with mRNAs
encoding proteins required for cell wall organization, and
some of which require the presence of Ssd1p; translational
repressor of cell wall-related proteins; interacts
physically and genetically with Xrn1p; homologous to
mammalian eIF2A"
/codon_start=1
/product="translation initiation factor 2A"
/protein_id="NP_011568.3"
/db_xref="GeneID:852945"
/db_xref="SGD:S000003286"
/translation="MSSQFFLKTSQDIELFQSYPTFEQSNTNSKDFPVISSVLSPCGR
FLALSTKENVKVFTGPCLDNVLLTMKLSDVYDLHFSPAGNYLSTWERASIQDPNHKNV
KVWYLNKPFKKDCVSEDIVPAYEYQAKSQSGWFLQFSKLDNYGLRLFKHDLKIVKLSS
ANADNFDFQSPFAVLSDDETSQHFTTYLISPAEHPTICTFTPEKGGKPAQLIIWALSE
GKITKKIASKTFFKADSCQLKWNPLGNAILCLAITDFDSSNKSYYGENTLYLLSFQGV
NGTLGGNSVRVSLTTGPVHDFTWSPTSRQFGVIAGYMPATISFFDLRGNVVHSLPQQA
KNTMLFSPSGHYILIAGFGNLQGSVEILDRLDKFKCVSKFDATNTSVCKWSPGGEFIM
TATTSPRLRVDNGVKIWHVSGSLVFVKEFKELLKVDWRSPCNYKTLENKDEAFFENHI
INNWEPLPDSTTSSLDPKISNKSELQIHSSVQEYISQHPSREASSNGNGSKAKAGGAY
KPPHARRTGGGRIVPGVPPGAAKKTIPGLVPGMSANKDANTKNRRRRANKKSSETSPD
STPAPSAPASTNAPTNNKETSPEEKKIRSLLKKLRAIETLKERQAVGDKLEDTQVLKI
QTEEKVLKDLEKLGWKDE"
gene <599417..>601141
/gene="MUP1"
/locus_tag="YGR055W"
/db_xref="GeneID:852946"
mRNA <599417..>601141
/gene="MUP1"
/locus_tag="YGR055W"
/product="Mup1p"
/transcript_id="NM_001181184.1"
/db_xref="GeneID:852946"
CDS 599417..601141
/gene="MUP1"
/locus_tag="YGR055W"
/experiment="EXISTENCE:direct assay:GO:0000102
L-methionine secondary active transmembrane transporter
activity [PMID:8893857]"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:25942624]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:1903692
methionine import across plasma membrane [PMID:8893857]"
/experiment="EXISTENCE:mutant phenotype:GO:0000101 sulfur
amino acid transport [PMID:8893857]"
/experiment="EXISTENCE:mutant phenotype:GO:1903692
methionine import across plasma membrane [PMID:8893857]"
/note="High affinity methionine permease; integral
membrane protein with 13 putative membrane-spanning
regions; involved in both methionine and cysteine uptake;
subject to transport-activity dependent,
methionine-induced endocytosis, vacuolar delivery and
degradation; starvation-induced endocytosis is
transport-activity independent"
/codon_start=1
/product="Mup1p"
/protein_id="NP_011569.1"
/db_xref="GeneID:852946"
/db_xref="SGD:S000003287"
/translation="MSEGRTFLSQLNVFNKENYQFSSSTTKKEVSNSTVDADNGASDF
EAGQQFATELDQGEKQLGILSCIGLICNRMLGTGVFAVSSTIYTLCGSVGLALIMWAV
GAIIAISGLYVYMEFGTAIPKNGGEKNYLEAIFRKPKFFITCMYAAYIFFLGWAAGNS
INTAIMFLTAADTEVTKWNQRGIGVAVVFFAFLINSLNVKIGLYLQNILGIFKIGIVL
FISITGWVALGGGLKDGYQSHNFRNAFEGTETATAYGIVNALYSVIWSFVGYSNVNYA
LGEVKNPVRTLKIAGPTSMVFLAIIYIFVNIAYFAVVPKDKLISSKLILAADFFDIVF
GGQAKRAAAALVGLSALGNVLSVIFSQGRIIQQLGREGVLPFSNFFASSKPFNSPMVG
LFQHFIVCTVTILAPPPGDAYLLVQNLISYPMNIINFAISAGLLWIYWQRRQGKIEWN
PPIKAGVFVTGFFTLSNLYLIIAPYVPPSNGESVYSSMPYWIHCVIAWGIFFFGGVYY
VVWAQLLPRWGHYKLVSKDVLGEDGFWRVKIAKVYDDTIGDVDTQEDGVIETNIIEHY
KSEQEKSL"
gene <601661..>604447
/gene="RSC1"
/locus_tag="YGR056W"
/db_xref="GeneID:852947"
mRNA <601661..>604447
/gene="RSC1"
/locus_tag="YGR056W"
/product="RSC subunit protein RSC1"
/transcript_id="NM_001181185.1"
/db_xref="GeneID:852947"
CDS 601661..604447
/gene="RSC1"
/locus_tag="YGR056W"
/experiment="EXISTENCE:curator inference:GO:0008094
ATP-dependent activity, acting on DNA [PMID:10619019]"
/experiment="EXISTENCE:direct assay:GO:0006337 nucleosome
disassembly [PMID:16492771]"
/experiment="EXISTENCE:direct assay:GO:0006368
transcription elongation by RNA polymerase II
[PMID:17081996]"
/experiment="EXISTENCE:direct assay:GO:0016586 RSC-type
complex [PMID:10619019]"
/experiment="EXISTENCE:mutant phenotype:GO:0006302
double-strand break repair [PMID:16024655]"
/experiment="EXISTENCE:physical interaction:GO:0006303
double-strand break repair via nonhomologous end joining
[PMID:15870268]"
/note="Component of the RSC chromatin remodeling complex;
required for expression of mid-late sporulation-specific
genes; contains two essential bromodomains, a
bromo-adjacent homology (BAH) domain, and an AT hook; RSC1
has a paralog, RSC2, that arose from the whole genome
duplication"
/codon_start=1
/product="RSC subunit protein RSC1"
/protein_id="NP_011570.1"
/db_xref="GeneID:852947"
/db_xref="SGD:S000003288"
/translation="MVEQDNGFLQKLLKTQYDAVFHLKDENGIEIYPIFNVLPPKKEY
PDYYIIIRNPISLNTLKKRLPHYTSPQDFVNDFAQIPWNAMTYNAKDSVIYKYAILLE
SFIKGKIVHNIRKHYPEVTYPSLGRIPEIFAESMQPSDLSSNPINTQENDEKAGLNPE
MKMAFAKLDSSITERKPTNQDYRMQQKNSPAFPTHSASITPQPLASPTPVVNYANITS
AHPKTHVRRGRPPVIDLPYVLRIKNILKMMRREVDQNNKTLTLCFEKLPDRNEEPTYY
SVITDPICLMDIRKKVKSRKYRNFHTFEEDFQLMLTNFKLYYSQDQSNIIRAQLLEKN
FNRLVRIELSKPDEDYLPEGELRYPLDDVEINDEKYQIGDWVLLHNPNDINKPIVGQI
FRLWSTTDGNKWLNACWYFRPEQTVHRVDRLFYKNEVMKTGQYRDHPIQDIKGKCYVI
HFTRFQRGDPSTKVNGPQFVCEFRYNESDKVFNKIRTWKACLPEELRDQDEPTIPVNG
RKFFKYPSPIADLLPANATLNDKVPEPTEGAPTAPPLVGAVYLGPKLERDDLGEYSTS
DDCPRYIIRPNDPPEEGKIDYETGTIITDTLTTSSMPRVNSSSTIRLPTLKQTKSIPS
SNFRSSSNTPLLHQNFNQTSNFLKLENMNNSSHNLLSHPSVPKFQSPSLLEQSSRSKY
HSAKKQTQLSSTAPKKPASKSFTLSSMINTLTAHTSKYNFNHIVIEAPGAFVVPVPME
KNIRTIQSTERFSRSNLKNAQNLGNTAINDINTANEQIIWFKGPGVKITERVIDSGND
LVRVPLNRWFCKNKRRKLDYEDIEEDVMEPPNDFSEDMIANIFNPPPSLNLDMDLNLS
PSSNNSSNFMDLSTIASGDNDGKECDTAEESEDENEDTEDEHEIEDIPTTSAFGLNSS
AEYLAFRLREFNKL"
gene complement(<604773..>605501)
/gene="LST7"
/locus_tag="YGR057C"
/db_xref="GeneID:852948"
mRNA complement(<604773..>605501)
/gene="LST7"
/locus_tag="YGR057C"
/product="Lst7p"
/transcript_id="NM_001181186.3"
/db_xref="GeneID:852948"
CDS complement(604773..605501)
/gene="LST7"
/locus_tag="YGR057C"
/experiment="EXISTENCE:direct assay:GO:0005096 GTPase
activator activity [PMID:26387955]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:26387955]"
/experiment="EXISTENCE:direct assay:GO:0005774 vacuolar
membrane [PMID:26387955]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:1990877
FNIP-folliculin RagC/D GAP [PMID:26387955]"
/experiment="EXISTENCE:mutant phenotype:GO:0071230
cellular response to amino acid stimulus [PMID:26387955]"
/experiment="EXISTENCE:mutant phenotype:GO:1904263
positive regulation of TORC1 signaling [PMID:26387955]"
/note="Subunit of the Lst4p-Lst7p GTPase activating
protein complex for Gtr2p; stimulates the GTPase activity
of Rag family GTPase Gtr2p, within the context of the
Gtr1p-Gtr2p heterodimer, after amino acid stimulation;
required for activation of TORC1 in response to amino acid
stimulation; recruited to the vacuolar membrane during
amino acid starvation and released from the membrane by
TORC1; required for the transport of amino acid permease
Gap1p from the Golgi to the cell surface"
/codon_start=1
/product="Lst7p"
/protein_id="NP_011571.4"
/db_xref="GeneID:852948"
/db_xref="SGD:S000003289"
/translation="MSSTVISLAHFCDKHGPRIISVTQSAEKGTLGEELLVPDYPTES
YCESCLLQFPEESTRSMRCFIEDVPFITTQYSSIRYQLLNSIIKRAFSEETMIYDNMP
FIFFDDLRGLNLVIGFKLYDENARGNERRYCFILTVDSRSHDDSMKMLSEHWNFIIGG
FDKMIAYIKNIHKSEFLGKNKTVENNLETLNNNAFIGSYLRANKSKFGRNLVSLTDDK
FLFVRIHKWNSFLLHTVMNENKLP"
gene <606136..>607143
/gene="PEF1"
/locus_tag="YGR058W"
/db_xref="GeneID:852949"
mRNA <606136..>607143
/gene="PEF1"
/locus_tag="YGR058W"
/product="Pef1p"
/transcript_id="NM_001181187.2"
/db_xref="GeneID:852949"
CDS 606136..607143
/gene="PEF1"
/locus_tag="YGR058W"
/experiment="EXISTENCE:direct assay:GO:0003400 regulation
of COPII vesicle coating [PMID:22792405]"
/experiment="EXISTENCE:direct assay:GO:0005509 calcium ion
binding [PMID:17640275]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22792405]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:17640275]"
/experiment="EXISTENCE:direct assay:GO:0008270 zinc ion
binding [PMID:17640275]"
/experiment="EXISTENCE:direct assay:GO:0030427 site of
polarized growth [PMID:17640275]"
/experiment="EXISTENCE:mutant phenotype:GO:0003400
regulation of COPII vesicle coating [PMID:22792405]"
/experiment="EXISTENCE:mutant phenotype:GO:0007120 axial
cellular bud site selection [PMID:17640275]"
/experiment="EXISTENCE:mutant phenotype:GO:0007121 bipolar
cellular bud site selection [PMID:17640275]"
/experiment="EXISTENCE:physical interaction:GO:0003400
regulation of COPII vesicle coating [PMID:22792405]"
/note="Penta-EF-hand protein; required for polar bud
growth and cell wall abscission; binds calcium and zinc
with different affinity; localizes to bud site in G1, bud
neck in G2; binds to Sec31p and modulates COPII coat
assembly"
/codon_start=1
/product="Pef1p"
/protein_id="NP_011572.2"
/db_xref="GeneID:852949"
/db_xref="SGD:S000003290"
/translation="MCAKKLKYAAGDDFVRYATPKEAMEETRREFEKEKQRQQQIKVT
QAQTPNTRVHSAPIPLQTQYNKNRAENGHHSYGSPQSYSPRHTKTPVDPRYNVIAQKP
AGRPIPPAPTHYNNLNTSAQRIASSPPPLIHNQAVPAQLLKKVAPASFDSREDVRDMQ
VATQLFHNHDVKGKNRLTAEELQNLLQNDDNSHFCISSVDALINLFGASRFGTVNQAE
FIALYKRVKSWRKVYVDNDINGSLTISVSEFHNSLQELGYLIPFEVSEKTFDQYAEFI
NRNGTGKELKFDKFVEALVWLMRLTKLFRKFDTNQEGIATIQYKDFIDATLYLGRFLP
H"
gene <607563..>609101
/gene="SPR3"
/locus_tag="YGR059W"
/db_xref="GeneID:852950"
mRNA <607563..>609101
/gene="SPR3"
/locus_tag="YGR059W"
/product="septin SPR3"
/transcript_id="NM_001181188.3"
/db_xref="GeneID:852950"
CDS 607563..609101
/gene="SPR3"
/locus_tag="YGR059W"
/experiment="EXISTENCE:direct assay:GO:0005619 ascospore
wall [PMID:8636217]"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:18826657]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0031105 septin
complex [PMID:24390141|PMID:18826657]"
/experiment="EXISTENCE:mutant phenotype:GO:0030437
ascospore formation [PMID:8636217]"
/note="Sporulation-specific homolog of the CDC3/10/11/12
family of genes; septin protein involved in sporulation;
regulated by ABFI; the yeast CDC3/10/11/12 family is a
family of bud neck microfilament genes"
/codon_start=1
/product="septin SPR3"
/protein_id="NP_011573.3"
/db_xref="GeneID:852950"
/db_xref="SGD:S000003291"
/translation="MKSKGSRLSTDCPVEFPKIVSGFAEEVKIRRQSSQGQYAVDSHP
PKSPELKHRRQRSSSFVNGKCRNRDLPLLDNKKAQEINTNSHGQDIGIKNLPRQRELL
NAKNGIDFTLMVAGQSGLGKTTFINSLFSTSLIDDDIKENKPIIRYKSIVEGDGTHLN
FNVIDTPGFGNNMDNAFTWRTMVNYIDEEIRSYIFQEEQPDRTKMVDNRVHCCLYFLR
PSNKGIDTLDVVTMKKLAKRVNLIPVIAKSDLLTKEELKNFKTQVREIIRVQDIPVCF
FFGDEVLNATQDIFQKYPFSIIASNEYIFNEKGEKVKGRQYKWGAVDIENEKYCDFKI
LQKTIFDWNLIDLVESTEDYYEKCRSEMLRTRLLKARDCLTTKSVDITEEQRKFLEEE
MNFDEIEENKLKNYKCYEIINKTVMDKVATEWDPEFITRQLEAKKKFNELSNREISKF
RDWKKSLFMEQENFNQEIEQLNHKLENLQLECQDLEYKLLIGKSSNSHSTDSATLVNV
HIKR"
gene 609584..609696
/gene="SNR48"
/locus_tag="YNCG0026W"
/db_xref="GeneID:9164898"
ncRNA 609584..609696
/ncRNA_class="snoRNA"
/gene="SNR48"
/locus_tag="YNCG0026W"
/product="SNR48"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:10024243]"
/experiment="EXISTENCE:curator inference:GO:0031428 box
C/D methylation guide snoRNP complex [PMID:10024243]"
/experiment="EXISTENCE:mutant phenotype:GO:0030562 rRNA
2'-O-ribose methylation guide activity [PMID:10024243]"
/experiment="EXISTENCE:mutant phenotype:GO:0031167 rRNA
methylation [PMID:10024243]"
/note="C/D box small nucleolar RNA (snoRNA); guides
2'-O-methylation of large subunit (LSU) rRNA at positions
G2791 and G2793"
/transcript_id="NR_132183.1"
/db_xref="GeneID:9164898"
/db_xref="SGD:S000006440"
gene <610564..>611493
/gene="ERG25"
/locus_tag="YGR060W"
/db_xref="GeneID:852951"
mRNA <610564..>611493
/gene="ERG25"
/locus_tag="YGR060W"
/product="methylsterol monooxygenase"
/transcript_id="NM_001181189.3"
/db_xref="GeneID:852951"
CDS 610564..611493
/gene="ERG25"
/locus_tag="YGR060W"
/experiment="EXISTENCE:direct assay:GO:0000254 C-4
methylsterol oxidase activity
[PMID:12880870|PMID:8663358]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:8663358]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:8663358]"
/experiment="EXISTENCE:direct assay:GO:0006696 ergosterol
biosynthetic process [PMID:12880870|PMID:8663358]"
/experiment="EXISTENCE:mutant phenotype:GO:0000254 C-4
methylsterol oxidase activity [PMID:8552601]"
/experiment="EXISTENCE:mutant phenotype:GO:0006696
ergosterol biosynthetic process [PMID:8552601]"
/experiment="EXISTENCE:physical interaction:GO:0005783
endoplasmic reticulum [PMID:12119386]"
/note="C-4 methyl sterol oxidase; catalyzes the first of
three steps required to remove two C-4 methyl groups from
an intermediate in ergosterol biosynthesis; mutants
accumulate the sterol intermediate 4,4-dimethylzymosterol;
human MSMO1 functionally complements the growth defect
caused by repression of ERG25 expression"
/codon_start=1
/product="methylsterol monooxygenase"
/protein_id="NP_011574.3"
/db_xref="GeneID:852951"
/db_xref="SGD:S000003292"
/translation="MSAVFNNATLSGLVQASTYSQTLQNVAHYQPQLNFMEKYWAAWY
SYMNNDVLATGLMFFLLHEFMYFFRCLPWFIIDQIPYFRRWKLQPTKIPSAKEQLYCL
KSVLLSHFLVEAIPIWTFHPMCEKLGITVEVPFPSLKTMALEIGLFFVLEDTWHYWAH
RLFHYGVFYKYIHKQHHRYAAPFGLSAEYAHPAETLSLGFGTVGMPILYVMYTGKLHL
FTLCVWITLRLFQAVDSHSGYDFPWSLNKIMPFWAGAEHHDLHHHYFIGNYASSFRWW
DYCLDTESGPEAKASREERMKKRAENNAQKKTN"
gene complement(<611889..>615965)
/gene="ADE6"
/locus_tag="YGR061C"
/db_xref="GeneID:852952"
mRNA complement(<611889..>615965)
/gene="ADE6"
/locus_tag="YGR061C"
/product="phosphoribosylformylglycinamidine synthase"
/transcript_id="NM_001181190.1"
/db_xref="GeneID:852952"
CDS complement(611889..615965)
/gene="ADE6"
/locus_tag="YGR061C"
/EC_number="6.3.5.3"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0004642
phosphoribosylformylglycinamidine synthase activity
[PMID:9331966]"
/experiment="EXISTENCE:mutant phenotype:GO:0006164 purine
nucleotide biosynthetic process [PMID:1743513]"
/note="Formylglycinamidine-ribonucleotide
(FGAM)-synthetase; catalyzes a step in the 'de novo'
purine nucleotide biosynthetic pathway"
/codon_start=1
/product="phosphoribosylformylglycinamidine synthase"
/protein_id="NP_011575.1"
/db_xref="GeneID:852952"
/db_xref="SGD:S000003293"
/translation="MTDYILPGPKALSQFRVDNLIKDINSYTNSTSVINELRSCYIHY
VNGIAQNLSEQDTKLLEVLLTYDSALDIANDPLARQLNDAVANNLPSSALGEDTYLIR
VVPRSGTISPWSSKATNIAHVCGLQDKVQRIERGLALLIKTVPGFPLLENLNDISLKC
VYDRMTQQLYLTEPPNTMSIFTHEEPKPLVHVPLTPKDTKQSPKDILSKANTELGLAL
DSGEMEYLIHAFVETMKRDPTDVELFMFAQVNSEHCRHKIFNADWTIDGIKQQFTLFQ
MIRNTHKLNPEYTISAYSDNAAVLDSENDAFFFAPNSTTKEWTSTKERIPLLIKVETH
NHPTAVSPFPGAATGSGGEIRDEGATGRGSKTKCGLSGFSVSDLLIPGNEQPWELNIG
KPYHIASALDIMIEAPLGSAAFNNEFGRPCINGYFRTLTTKVLNHQGKEEIRGFHKPI
MIAGGFGTVRPQFALKNTPITPGSCLIVLGGQSMLIGLGGGAASSVASGEGSADLDFA
SVQRGNPEMERRCQQVIDACVALGNNNPIQSIHDVGAGGLSNALPELVHDNDLGAKFD
IRKVLSLEPGMSPMEIWCNESQERYVLGVSPQDLSIFEEICKRERAPFAVVGHATAEQ
KLIVEDPLLKTTPIDLEMPILFGKPPKMSRETITEALNLPEANLSEIPSLQDAIQRVL
NLPSVGSKSFLITIGDRSVTGLIDRDQFVGPWQVPVADVGVTGTSLGETIISTGEAMA
MGEKPVNALISASASAKLSVAESLLNIFAADVKSLNHIKLSANWMSPASHQGEGSKLY
EAVQALGLDLCPALGVAIPVGKDSMSMKMKWDDKEVTAPLSLNITAFAPVFNTSKTWT
PLLNRNTDDSVLVLVDLSAKQETKSLGASALLQVYNQVGNKSPTVYDNAILKGFLESL
IQLHQQKEDIVLAYHDRSDGGLLITLLEMAFASRCGLEINIDGGDLESQLTNLFNEEL
GAVFQISAKNLSKFEKILNENGVAKEYISIVGKPSFQSQEIKIINSTTNDVIYANSRS
ELEQTWSKTSYEMQKLRDNPKTAEEEFASITDDRDPGLQYALTYNPADDMKIGLELSS
QRPKVAILREQGVNGQMEMAWCFQQAGFNSVDVTMTDLLEGRFHLDDFIGLAACGGFS
YGDVLGAGAGWAKSVLYHEGVRSQFSKFFNERQDTFAFGACNGCQFLSRLKDIIPGCE
NWPSFERNVSEQYEARVCMVQISQEKDNSSEESVFLNGMAGSKLPIAVAHGEGKATFS
KSAEQLEKFEKDGLCCIRYVDNYGNVTERFPFNPNGSTNGIAGIKSPNGRVLAMMPHP
ERVCRLEANSWYPEGKYEEWGGYGPWIRLFRSARRWVG"
gene complement(<616328..>617278)
/gene="COX18"
/locus_tag="YGR062C"
/gene_synonym="OXA2"
/db_xref="GeneID:852953"
mRNA complement(<616328..>617278)
/gene="COX18"
/locus_tag="YGR062C"
/gene_synonym="OXA2"
/product="membrane insertase COX18"
/transcript_id="NM_001181191.1"
/db_xref="GeneID:852953"
CDS complement(616328..617278)
/gene="COX18"
/locus_tag="YGR062C"
/gene_synonym="OXA2"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:11950926]"
/experiment="EXISTENCE:genetic interaction:GO:0032977
membrane insertase activity [PMID:17922846]"
/experiment="EXISTENCE:genetic interaction:GO:0032979
protein insertion into mitochondrial inner membrane from
matrix [PMID:17922846|PMID:11950926]"
/experiment="EXISTENCE:mutant phenotype:GO:0032977
membrane insertase activity [PMID:11950926]"
/experiment="EXISTENCE:mutant phenotype:GO:0032979 protein
insertion into mitochondrial inner membrane from matrix
[PMID:11950926]"
/experiment="EXISTENCE:physical interaction:GO:0032977
membrane insertase activity [PMID:17452441]"
/experiment="EXISTENCE:physical interaction:GO:0032979
protein insertion into mitochondrial inner membrane from
matrix [PMID:17452441]"
/note="Protein required for membrane insertion of
C-terminus of Cox2p; mitochondrial integral inner membrane
protein; interacts genetically and physically with Mss2p
and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p,
and E. coli YidC; respiratory defect of the null mutant is
functionally complemented by human COX18 carrying the
N-terminal 54 amino acids of S. cerevisiae Cox18p"
/codon_start=1
/product="membrane insertase COX18"
/protein_id="NP_011576.1"
/db_xref="GeneID:852953"
/db_xref="SGD:S000003294"
/translation="MLKRLANRQNGFASFSCSSVGLRYGRTNPSTKRSFSLFQSVADT
FLTVHEASHIPWIVLVPLTTMTLRTLVTLPFSIWQRRRILKQQELRKLVQPITPIIKL
RLAAVTNKKSRNAARISSNGSFMPLQLQNAGVLTPEQITLLAVKETRKRQKKLFKKYN
VPLWKNALLPMVQIPLWVTVSMGIRTLTETQLIESFYPSWFSALGFSSFDLSSPLVAM
PLLAPILVGTLAVLNVELNGRLMFSSSLSSQGIKTISRNSTRVQEAMTSILNVSRLGC
VVMLAMSSQAPFLLSLYWISSQLFSLVQNIILNWIYPYQR"
gene complement(<617516..>617824)
/gene="SPT4"
/locus_tag="YGR063C"
/db_xref="GeneID:852955"
mRNA complement(<617516..>617824)
/gene="SPT4"
/locus_tag="YGR063C"
/product="transcription elongation factor SPT4"
/transcript_id="NM_001181192.3"
/db_xref="GeneID:852955"
CDS complement(617516..617824)
/gene="SPT4"
/locus_tag="YGR063C"
/experiment="EXISTENCE:direct assay:GO:0000182 rDNA
binding [PMID:16908835]"
/experiment="EXISTENCE:direct assay:GO:0000776 kinetochore
[PMID:15057281]"
/experiment="EXISTENCE:direct assay:GO:0000993 RNA
polymerase II complex binding [PMID:26945063]"
/experiment="EXISTENCE:direct assay:GO:0003727
single-stranded RNA binding [PMID:27376968]"
/experiment="EXISTENCE:direct assay:GO:0031934 mating-type
region heterochromatin [PMID:15057281]"
/experiment="EXISTENCE:direct assay:GO:0032968 positive
regulation of transcription elongation by RNA polymerase
II [PMID:26945063]"
/experiment="EXISTENCE:direct assay:GO:0033553 rDNA
heterochromatin [PMID:16908835]"
/experiment="EXISTENCE:direct assay:GO:0044877
protein-containing complex binding [PMID:26945063]"
/experiment="EXISTENCE:genetic interaction:GO:0006370
7-methylguanosine mRNA capping [PMID:12556496]"
/experiment="EXISTENCE:genetic interaction:GO:0090262
regulation of transcription-coupled nucleotide-excision
repair [PMID:20042611]"
/experiment="EXISTENCE:genetic interaction:GO:2001208
negative regulation of transcription elongation by RNA
polymerase I [PMID:21467039]"
/experiment="EXISTENCE:mutant phenotype:GO:0006325
chromatin organization [PMID:15057281]"
/experiment="EXISTENCE:mutant phenotype:GO:0006355
regulation of DNA-templated transcription [PMID:9450930]"
/experiment="EXISTENCE:mutant phenotype:GO:0008298
intracellular mRNA localization [PMID:20713510]"
/experiment="EXISTENCE:mutant phenotype:GO:0010507
negative regulation of autophagy [PMID:31462158]"
/experiment="EXISTENCE:mutant phenotype:GO:0031507
heterochromatin formation [PMID:15057281]"
/experiment="EXISTENCE:mutant phenotype:GO:0032968
positive regulation of transcription elongation by RNA
polymerase II [PMID:12554661]"
/experiment="EXISTENCE:mutant phenotype:GO:2000232
regulation of rRNA processing [PMID:21949810]"
/experiment="EXISTENCE:mutant phenotype:GO:2001209
positive regulation of transcription elongation by RNA
polymerase I [PMID:16908835]"
/experiment="EXISTENCE:physical interaction:GO:0032044
DSIF complex [PMID:9450930]"
/note="Spt4p/5p (DSIF) transcription elongation factor
complex subunit; Spt4/5 binds ssRNA in a sequence-specific
manner and along with RNAP I and II regulates
transcriptional elongation, RNA processing, quality
control, and transcription-coupled repair; localizes to
kinetochores and heterochromatin, influencing chromosomal
dynamics and silencing; required for transcription through
long trinucleotide repeats in ORFs and non-protein coding
regions; accelerates senescence through rDNA instability"
/codon_start=1
/product="transcription elongation factor SPT4"
/protein_id="NP_011577.3"
/db_xref="GeneID:852955"
/db_xref="SGD:S000003295"
/translation="MSSERACMLCGIVQTTNEFNRDGCPNCQGIFEEAGVSTMECTSP
SFEGLVGMCKPTKSWVAKWLSVDHSIAGMYAIKVDGRLPAEVVELLPHYKPRDGSQVE
"
gene complement(<618077..>619858)
/gene="VHT1"
/locus_tag="YGR065C"
/db_xref="GeneID:852956"
mRNA complement(<618077..>619858)
/gene="VHT1"
/locus_tag="YGR065C"
/product="Vht1p"
/transcript_id="NM_001181194.1"
/db_xref="GeneID:852956"
CDS complement(618077..619858)
/gene="VHT1"
/locus_tag="YGR065C"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:10373489]"
/experiment="EXISTENCE:direct assay:GO:0015225 biotin
transmembrane transporter activity [PMID:10373489]"
/experiment="EXISTENCE:direct assay:GO:0015878 biotin
transport [PMID:10373489]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="High-affinity plasma membrane H+-biotin (vitamin H)
symporter; mutation results in fatty acid auxotrophy; 12
transmembrane domain containing major facilitator
subfamily member; mRNA levels negatively regulated by iron
deprivation and biotin"
/codon_start=1
/product="Vht1p"
/protein_id="NP_011579.1"
/db_xref="GeneID:852956"
/db_xref="SGD:S000003297"
/translation="MTISNKSWRSYFPHLRKLPEDDQYLYSDDTNSSIIAEEELHHSV
DKSSKTDVTAETTAVEPHPHNLRHDLPYEVRDEAGRKWWKYFDEFEYRVNKEYKKSRK
WYEFLYPNHTTQTKAERRLLYKLDIIIALYFFMLCWSKSVDLNNYTNAYVSNMKEDLN
MKGNDYVYTSTIANVGAIVFQLPFMYLLPRFPSHIILPVMDLGWTWFTFACYRANSLA
ELRAYRFILSAFGAAYYPVSQYILGCWYAPDEINSRVCLFFCGQQLGSVTSGLLQSRI
FKSLNGVHGLAGWRWMFLIDAIAISLPTAIIGFFVIPGVPSKCYSLFLTDEEIRIARA
RNKRNQIKDGVDKSKLAPLWSRKLWKKVFCTPAFWVLVVFDTCSWNNMTAYSGSYTLW
LKSNTKYSIAQVNNLSVIPACLGFAYVIFCAFGADLFRCKWIFMVFAAIMNTVSCALL
IKWDIPSKAKWYAFFTTYFSVAASPCLWSFINDFLRFDPQVKAITWIAIYSFSQSTYA
WIPTLAWPTVESPRFKTGYTVSLIFGAIYGLWTFVVLFFYKRNEKKHALGNGIILYDS
NKGEELPEFVKKNMEERDGYYYLKRSS"
gene complement(<620921..>621799)
/gene="GID10"
/locus_tag="YGR066C"
/db_xref="GeneID:852957"
mRNA complement(<620921..>621799)
/gene="GID10"
/locus_tag="YGR066C"
/product="Gid10p"
/transcript_id="NM_001181195.3"
/db_xref="GeneID:852957"
CDS complement(620921..621799)
/gene="GID10"
/locus_tag="YGR066C"
/experiment="EXISTENCE:direct assay:GO:0006970 response to
osmotic stress [PMID:31337681]"
/experiment="EXISTENCE:direct assay:GO:0030163 protein
catabolic process [PMID:31337681]"
/experiment="EXISTENCE:direct assay:GO:0034657 GID complex
[PMID:31337681]"
/experiment="EXISTENCE:direct assay:GO:0042594 response to
starvation [PMID:31337681]"
/note="Recognition component (N-recognin) of the
Pro/N-degron pathway; recognizes and targets for
degradation proteins with N-terminal degradation signals;
expressed only under starvation or osmotic stress"
/codon_start=1
/product="Gid10p"
/protein_id="NP_011580.3"
/db_xref="GeneID:852957"
/db_xref="SGD:S000003298"
/translation="MTSLNIMGRKFILERAKRNDNIEEIYTSAYVSLPSSTDTRLPHF
KAKEEDCDVYEEGTNLVGKNAKYTYRSLGRHLDFLRPGLRFGGSQSSKYTYYTVEVKI
DTVNLPLYKDSRSLDPHVTGTFTIKNLTPVLDKVVTLFEGYVINYNQFPLCSLHWPAE
ETLDPYMAQRESDCSHWKRFGHFGSDNWSLTERNFGQYNHESAEFMNQRYIYLKWKER
FLLDDEEQENLMLDDNHHLEGASFEGFYYVCLDQLTGSVEGYYYHPACELFQKLELVP
TNCDALNTYSSGFEIA"
gene complement(<622372..>624786)
/locus_tag="YGR067C"
/db_xref="GeneID:852958"
mRNA complement(<622372..>624786)
/locus_tag="YGR067C"
/product="uncharacterized protein"
/transcript_id="NM_001181196.4"
/db_xref="GeneID:852958"
CDS complement(622372..624786)
/locus_tag="YGR067C"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding
[PMID:19111667|PMID:19158363]"
/note="hypothetical protein; contains a zinc finger motif
similar to that of Adr1p"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011581.4"
/db_xref="GeneID:852958"
/db_xref="SGD:S000003299"
/translation="MAAGQKKYICSFCLKPFSRSEHKIRHERSHAGVKPFQCQVCKHS
FVRRDLLQRHIRTVHRTFLLSSYASMTGDKADIPVSLGVGDVDSTSPRDIKMETLVNS
MIKVNSGLINIHYHSSNVEKMDKQQRCVIGKESSSLKKGKSRFKQVKSRLESSISVKI
LQEYSLDFISSRDILTFFRMGVSHLVENKIFQNFFPDLFSSLQNDELVESFWINKPFG
LIIACLGMSISLNQDSQKLWFICCTNLYASSSKHDNDFDTEDILSQTEQHDVFALILF
YSLLVMLENNIPVSNSIKKFDVFSMLQDILKPFTVASSSYHYLNSKENAWFIFDLWVN
ILRDSNNFNNDSLLIFGWFVNQEFISSNPLKDFIYKGPSMSTTDLTLKHINILADSAY
VYFIIKKTYPQELPSDFRVHDLLVYLNECFVMQQPIKPETSANPSLFANVMNARITDC
KSKSNWLLWETIWFEFINNLTLRNGTTRNIWFIDNFPQVSTSCLLHHSSSFVDETLIT
TNLSIISMLLNLKSFTLASLNPRNIQLITDIVSFQLKLFSSELIASSDVSPSQVSQLL
VNPNVHLMLYFWFDTIYVQRQSYLSSTEKEEFEKVEVFVNDYIITHQKNLVTDLHSIL
FDFWSDSFIAYHILLHAIVSSLRDNILYPYLIYSPHLNDQTKALLTDISNWSCFALQQ
PFRKTSRGSLSGASDMKSFSVASCLPLSPNFLKRDSNCNKILLPPLDIKAIEPISTSN
YTYVNSAPKQQEKEQPLLRATGNNINLVQTIVVPPQVNMESQEFSASSTDNKQSKNIE
IFSQIK"
gene complement(<625324..>627084)
/gene="ART5"
/locus_tag="YGR068C"
/db_xref="GeneID:852960"
mRNA complement(<625324..>627084)
/gene="ART5"
/locus_tag="YGR068C"
/product="Art5p"
/transcript_id="NM_001181197.1"
/db_xref="GeneID:852960"
CDS complement(625324..627084)
/gene="ART5"
/locus_tag="YGR068C"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:physical interaction:GO:0031625
ubiquitin protein ligase binding [PMID:18976803]"
/note="Protein proposed to regulate endocytosis of plasma
membrane proteins; regulates by recruiting the ubiquitin
ligase Rsp5p to its target in the plasma membrane;
SWAT-GFP and mCherry fusion proteins localize to the
cytosol"
/codon_start=1
/product="Art5p"
/protein_id="NP_011582.1"
/db_xref="GeneID:852960"
/db_xref="SGD:S000003300"
/translation="MFSLSSLSSSGGHSEQKERERISYFDIRINSPYKDIILIQGSPL
ELSSIPLSGNLVISVKNEIVVKKISLRLVGRFKLEFLQVGRYKKNSSSLASLVKEKRK
IFECYWDNLLVSSKGDVLVGGENAENQHNSSSGRSTSNQDMDTSGNAIFLSKRSLSSP
VFNKIIRRKTHSSHRKILELPENGVTGTPFEGLRENARSRSSSSNTLNNNSHSYSNRD
GSGSSYLFLMKRGNYELPFNTMLPPEVCETIEGLQSGSILYSFEAIIDGRQLWDTDLS
VHTSPHGPIGSTSTSGNGMRTKNKIIIKKFKYLRILRTLSMDNLAMQEEISVGNTWRD
KLQYETSIPSRAVPIGSTTPVKIKIFPFEKNIRLDRIEMALIQYYAMKDSSAQIYDDE
IAVMKITHLADFGPLTDKLDVDCPFTIPDNLKQITQDCCLQDNLIRVMHKLQVRILLQ
RQVDGEYKNLEIKAQLPMLLFISPHLPMKGRLVLFDKHDGKIHFRPGELVPLFLTTYP
AQGLTPGVELNSTTTAHLALPQPPPNYHESTNDHLMPALQPLGADSVVLTVPSYEQAQ
AQASASSYVTGSVPAYCDDD"
gene <627806..>631273
/gene="ROM1"
/locus_tag="YGR070W"
/gene_synonym="SKC1"
/db_xref="GeneID:852961"
mRNA <627806..>631273
/gene="ROM1"
/locus_tag="YGR070W"
/gene_synonym="SKC1"
/product="Rho family guanine nucleotide exchange factor
ROM1"
/transcript_id="NM_001181199.2"
/db_xref="GeneID:852961"
CDS 627806..631273
/gene="ROM1"
/locus_tag="YGR070W"
/gene_synonym="SKC1"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22065638]"
/experiment="EXISTENCE:genetic interaction:GO:0005085
guanyl-nucleotide exchange factor activity [PMID:8641285]"
/experiment="EXISTENCE:genetic interaction:GO:0007264
small GTPase-mediated signal transduction [PMID:8641285]"
/note="Guanine nucleotide exchange factor (GEF) for Rho1p;
mutations are synthetically lethal with mutations in rom2,
which also encodes a GEP; ROM1 has a paralog, ROM2, that
arose from the whole genome duplication"
/codon_start=1
/product="Rho family guanine nucleotide exchange factor
ROM1"
/protein_id="NP_011584.2"
/db_xref="GeneID:852961"
/db_xref="SGD:S000003302"
/translation="MNSNELDLRNKYFYEIFGKKRKSDTSTPTQLFSGSKVQTNINEI
SITNDEDEDSTEDENKASLKDYTLGHDTGARYRIAPDCSSHQLKASPVLHISTNLNSS
PQSFTGDQISPTNKKISINDSTRQDKGNSCTTTSSPSQKRSNVLLPHVRKHSSPSLLS
FSKNSGSHMGDPNQLSTPPTPKSAGHTMELHSSFNGKHSSSSTSSLFALESLKTQNRR
SSNSSNHSSQYRRHTNQHQRHHSRSKSSPVSLTEISMIKGTPLVYPALLSLIAIKFKQ
TIKLSTHKKMGLLYRDSFTGKQAIDTLCLIIGSLDRNLGMLIGKSLEAQKLFHDVLYD
HGVRDSVLEIYELSSESIFMAHQSQSSTSIANTFSSSSSSVNSLRTKTEIYGVFVPLT
HCYSSTCSLEKLCYSISCPNRLQQQANLHLKLGGGLKRNISLALDKEDDERISWTNSV
PKSVWESLSKQQIKRQEAIYELFTTEKKFVKSLEIIRDTFMKKLLETNIIPSDVRINF
VKHVFAHINEIYSVNREFLKALAQRQSLSPICPGIADIFLQYLPFFDPFLSYIASRPY
AKYLIETQRSVNPNFARFDDEVSNSSLRHGIDSFLSQGVSRPGRYSLLVREIIHFSDP
VTDKDDLQMLMKVQDLLKDLMKRIDRASGAAQDRYDVKVLKQKILFKNEYVNLGLNNE
KRKIKHEGLLSRKDVNKTDASFSGDIQFYLLDNMLLFLKSKAVNKWHQHTVFQRPIPL
PLLFICPAEDMPPIKRYVTENPNCSAGVLLPQYQTSNPKNAIVFAYYGTKQQYQVTLY
APQPAGLQTLIEKVKQEQKRLLDETKHITFKQMVGQFFHSYINTNRVNDVLICHAGKI
LLVATNMGLFVLNYATSINQKPVHLLHKISISQISVLEEYKVMILLIDKKLYGCPLDV
IDDAENADFLFRKNSKVLFKYVAMFKDGFCNGKRIIMIAHHFLHAVQLLIVNPLIFDF
NSGNFKKNLKAGLVDFSVDSEPLSFSFLENKICIGCKKNIKILNVPEVCDKNGFKMRE
LLNLHDNKVLANMYKETFKVVSMFPIKNSTFACFPELCFFLNKQGKREETKGCFHWEG
EPEQFACSYPYIVAINSNFIEIRHIENGELVRCVLGNKIRMLKSYAKKILYCYEDPQG
FEIIELLNF"
gene complement(<631481..>634063)
/gene="ENV11"
/locus_tag="YGR071C"
/db_xref="GeneID:852962"
mRNA complement(<631481..>634063)
/gene="ENV11"
/locus_tag="YGR071C"
/product="Env11p"
/transcript_id="NM_001181200.3"
/db_xref="GeneID:852962"
CDS complement(631481..634063)
/gene="ENV11"
/locus_tag="YGR071C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0006624
vacuolar protein processing [PMID:21912603]"
/experiment="EXISTENCE:mutant phenotype:GO:0007033 vacuole
organization [PMID:21912603]"
/note="Protein proposed to be involved in vacuolar
functions; mutant shows defect in CPY processing and
fragmented vacuoles; deletion mutant has increased
glycogen accumulation and displays elongated buds; green
fluorescent protein (GFP)-fusion protein localizes to the
nucleus; ENV11 has a paralog, VID22, that arose from the
whole genome duplication"
/codon_start=1
/product="Env11p"
/protein_id="NP_011585.3"
/db_xref="GeneID:852962"
/db_xref="SGD:S000003303"
/translation="MNTALDDLHGDLVTLEDNEIINNSDHSSSHSTSHEEEDEEEDDT
EDIELIEKDGNKILSSRIHPEDEIINDGLNIWIPVQMLKKNIAKFWSHFLAIEKKLTK
VKCKHCGEILTRSDASLTKTFRSHLKTKHNISANKNFYSMNFTVGDSNLKNNTSSTEI
TRRHGYDSLTFNSDQSFKCFDIGKLQSSNYLSISQLVAIVIASENLPLNFFENVSFKS
LLSKFHRIPPLTTNIIEESIIGLSKSIDELIRRSISRNDTQLPFTIHLSDSKESNQPL
YLKYSREIRAQLSNLDLSHLISVNFTELAGKRSLFSLQLFDNTNKVSKGLPLSIFVRK
TTDIDISVWQEQLNNLYSKYPGLQKSVISITLPQSHYTMVLENRNSHNFTFHSGSVRE
IKYHTCIVSELLHCFLQPLFNVPTESMLSSFSVAKENHSGGSLLDSLIDFSHIDLSST
ILGKICCLIEEVNLNDSLKSDFLLYCQNYTQPNCNELTSILSCNCDRFSALKSILEKF
ANLVPFFKSINSHLENESLSESDFRLINTVEETLRTFEQSIEYFASSAPLKFTHTLVF
IIKFELYLTEIIRSFKFTKSKKPFEKILARLLKVKDLYLLDDVNLIGAFLYPSIFQSK
SLLNEIFGTTSVNKIVHNMTKIVLRYLKNFINITNFRSSNSGGESGRNSGNNLLSDYE
AIFMKESRDVELLCNTKLTAPLTEDSLLVQIIRDDLLRYVNRIAHELPNAYHDYLNDN
DISFDGSHFTKHELSEENDSNSGEWCLNPMEETFDIHIPISDSIWNNYISSKNKIEVI
DILLQLLSVNSTSSIRSELSSLTANQDFSTKLSEETIKIKLLNSQFNLEKINFHSGSI
FDAC"
gene <634304..>635467
/gene="UPF3"
/locus_tag="YGR072W"
/gene_synonym="SUA6; SUP112"
/db_xref="GeneID:852963"
mRNA <634304..>635467
/gene="UPF3"
/locus_tag="YGR072W"
/gene_synonym="SUA6; SUP112"
/product="Upf3p"
/transcript_id="NM_001181201.1"
/db_xref="GeneID:852963"
CDS 634304..635467
/gene="UPF3"
/locus_tag="YGR072W"
/gene_synonym="SUA6; SUP112"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9763508]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:12196393]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:12196393]"
/experiment="EXISTENCE:genetic interaction:GO:0070478
nuclear-transcribed mRNA catabolic process, 3'-5'
exonucleolytic nonsense-mediated decay [PMID:12881429]"
/experiment="EXISTENCE:mutant phenotype:GO:0006310 DNA
recombination [PMID:23390378]"
/experiment="EXISTENCE:mutant phenotype:GO:0016567 protein
ubiquitination [PMID:18676617]"
/experiment="EXISTENCE:physical interaction:GO:0016567
protein ubiquitination [PMID:18676617]"
/note="Component of the nonsense-mediated mRNA decay (NMD)
pathway; along with Nam7p and Nmd2p; involved in decay of
mRNA containing nonsense codons; involved in telomere
maintenance"
/codon_start=1
/product="Upf3p"
/protein_id="NP_011586.1"
/db_xref="GeneID:852963"
/db_xref="SGD:S000003304"
/translation="MSNVAGELKNSEGKKKGRGNRYHNKNRGKSKNETVDPKKNENKV
NNATNATHNNSKGRRNNKKRNREYYNYKRKARLGKSTENEGFKLVIRLLPPNLTADEF
FAILRDNNNDDGDKQDIQGKLKYSDWCFFEGHYSSKVFKNSTYSRCNFLFDNLSDLEK
CANFIKTCKFIDNKDNITIPDMKLSPYVKKFTQTSKKDAALVGTIEEDEIFKTFMNSM
KQLNENDEYSFQDFSVLKSLEKEFSKSIELENKIAERTERVLTELVGTGDKVKNKNKK
KKNKNAKKKFKEEEASAKIPKKKRNRGKKKRENREKSTISKTKNSNVVIIEEAGKEVL
KQRKKKMLLQEKLKISNSSQPQSSSAQTQPSFQPKENLFVPRVKILHRDDTKK"
gene <635712..>636152
/gene="SMD1"
/locus_tag="YGR074W"
/gene_synonym="SPP92"
/db_xref="GeneID:852964"
mRNA <635712..>636152
/gene="SMD1"
/locus_tag="YGR074W"
/gene_synonym="SPP92"
/product="mRNA splicing protein SMD1"
/transcript_id="NM_001181203.3"
/db_xref="GeneID:852964"
CDS 635712..636152
/gene="SMD1"
/locus_tag="YGR074W"
/gene_synonym="SPP92"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005682 U5 snRNP
[PMID:11720284|PMID:11720285]"
/experiment="EXISTENCE:direct assay:GO:0005685 U1 snRNP
[PMID:9012791|PMID:9630245]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0046540 U4/U6 x U5
tri-snRNP complex [PMID:10377396|PMID:10449419]"
/experiment="EXISTENCE:direct assay:GO:0071004 U2-type
prespliceosome [PMID:16618970]"
/experiment="EXISTENCE:physical interaction:GO:0000243
commitment complex [PMID:10072386]"
/experiment="EXISTENCE:physical interaction:GO:0000398
mRNA splicing, via spliceosome [PMID:10072386]"
/experiment="EXISTENCE:physical interaction:GO:0003729
mRNA binding [PMID:10072386]"
/note="Core Sm protein Sm D1; part of heteroheptameric
complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and
Smx2p) that is part of the spliceosomal U1, U2, U4, and U5
snRNPs; relocalizes to the cytosol in response to hypoxia;
homolog of human Sm D1; protein abundance increases in
response to DNA replication stress"
/codon_start=1
/product="mRNA splicing protein SMD1"
/protein_id="NP_011588.3"
/db_xref="GeneID:852964"
/db_xref="SGD:S000003306"
/translation="MKLVNFLKKLRNEQVTIELKNGTTVWGTLQSVSPQMNAILTDVK
LTLPQPRLNKLNSNGIAMASLYLTGGQQPTASDNIASLQYINIRGNTIRQIILPDSLN
LDSLLVDQKQLNSLRRSGQIANDPSKKRRRDFGAPANKRPRRGL"
gene complement(<636147..>636875)
/gene="PRP38"
/locus_tag="YGR075C"
/db_xref="GeneID:852966"
mRNA complement(<636147..>636875)
/gene="PRP38"
/locus_tag="YGR075C"
/product="U4/U6-U5 snRNP complex subunit PRP38"
/transcript_id="NM_001181204.3"
/db_xref="GeneID:852966"
CDS complement(636147..636875)
/gene="PRP38"
/locus_tag="YGR075C"
/experiment="EXISTENCE:direct assay:GO:0046540 U4/U6 x U5
tri-snRNP complex [PMID:10377396|PMID:10449419]"
/experiment="EXISTENCE:mutant phenotype:GO:0000388
spliceosome conformational change to release U4 (or
U4atac) and U1 (or U11) [PMID:9582287]"
/experiment="EXISTENCE:mutant phenotype:GO:0046540 U4/U6 x
U5 tri-snRNP complex [PMID:9582287]"
/note="Unique component of the U4/U6.U5 tri-snRNP
particle; tri-snRNP is required for conformational changes
which result in the catalytic activation of the
spliceosome; dispensable for spliceosome assembly"
/codon_start=1
/product="U4/U6-U5 snRNP complex subunit PRP38"
/protein_id="NP_011589.3"
/db_xref="GeneID:852966"
/db_xref="SGD:S000003307"
/translation="MAVNEFQVESNISPKQLNNQSVSLVIPRLTRDKIHNSMYYKVNL
SNESLRGNTMVELLKVMIGAFGTIKGQNGHLHMMVLGGIEFKCILMKLIEIRPNFQQL
NFLLNVKNENGFDSKYIIALLLVYARLQYYYLNGNNKNDDDENDLIKLFKVQLYKYSQ
HYFKLKSFPLQVDCFAHSYNEELCIIHIDELVDWLATQDHIWGIPLGKCQWNKIYNSD
EESSSSESESNGDSEDDNDTSSES"
gene complement(<637104..>637577)
/gene="MRPL25"
/locus_tag="YGR076C"
/gene_synonym="AFO1; mL59; YMR26"
/db_xref="GeneID:852967"
mRNA complement(<637104..>637577)
/gene="MRPL25"
/locus_tag="YGR076C"
/gene_synonym="AFO1; mL59; YMR26"
/product="mitochondrial 54S ribosomal protein mL59 MRPL25"
/transcript_id="NM_001181205.1"
/db_xref="GeneID:852967"
CDS complement(637104..637577)
/gene="MRPL25"
/locus_tag="YGR076C"
/gene_synonym="AFO1; mL59; YMR26"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:12392552]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:12392552]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005762
mitochondrial large ribosomal subunit [PMID:12392552]"
/note="Mitochondrial ribosomal protein of the large
subunit; mutation confers increased replicative lifespan"
/codon_start=1
/product="mitochondrial 54S ribosomal protein mL59 MRPL25"
/protein_id="NP_011590.1"
/db_xref="GeneID:852967"
/db_xref="SGD:S000003308"
/translation="MSYKQYFDSLPLKLKSFFQRYPPSIKYSPVSTSTKAINANPFLP
NKHPVTQRFHDPKYSLRRMSDVYKLALRYGVEEFLPPIENTKKLFFEEKYNKKTLMKG
VLLPKGHKHELKLNEKLKKREEALKKVDELIASKKGSKYAKRVEKMKKNQSIGWF"
gene complement(<637744..>639513)
/gene="PEX8"
/locus_tag="YGR077C"
/gene_synonym="PAS6"
/db_xref="GeneID:852968"
mRNA complement(<637744..>639513)
/gene="PEX8"
/locus_tag="YGR077C"
/gene_synonym="PAS6"
/product="Pex8p"
/transcript_id="NM_001181206.1"
/db_xref="GeneID:852968"
CDS complement(637744..639513)
/gene="PEX8"
/locus_tag="YGR077C"
/gene_synonym="PAS6"
/experiment="EXISTENCE:direct assay:GO:0030674
protein-macromolecule adaptor activity [PMID:12667447]"
/experiment="EXISTENCE:direct assay:GO:1990429 peroxisomal
importomer complex [PMID:22375831]"
/experiment="EXISTENCE:mutant phenotype:GO:0016558 protein
import into peroxisome matrix [PMID:10652355]"
/note="Intraperoxisomal organizer of the peroxisomal
import machinery; organizes the formation of the
importomer complex, bridging the docking complex with the
RING finger complex; tightly associated with the lumenal
face of the peroxisomal membrane; essential for peroxisome
biogenesis; binds PTS1-signal receptor Pex5p, and
PTS2-signal receptor Pex7p"
/codon_start=1
/product="Pex8p"
/protein_id="NP_011591.1"
/db_xref="GeneID:852968"
/db_xref="SGD:S000003309"
/translation="MFDHDVEYLITALSSETRIQYDQRLLDEIAANVVYYVPRVKSPD
TLYRLVGALFRSQFIVQLPPLRLLHIVKDVFLWKLEVSEPTLPISKFYLVWNAVFESH
RATWNLSQLMVLDGVLVTYPSFKQLNNAYFIDESSNKTALYYRNWKLQLFSPIWAQLW
NTAIVRANLSIQHCLLIALALLFNQSNRSALLHGVDVSWNLVTEKLLDLLEEYVHGIV
QPMEIFSTDSVLSTNLNHLASCLTSSITRSNEATLVNSVRKLERICRYLSDTVASLKE
QQLDFKFQNVFILIILALKELSAMNMTILPNHKDTFYSMICLSLFHVHVLTQKIGTVG
FPSYDYVYDNLVTYFIVMDDLSKITTVLELMKRNNTKQDPNKLVFYINFLNKITNYYG
CRIRLPFITEFIEPLLHFDVFFSGKTGNTLDIEIKESIHTLTITVLSIDSSYSSQVAQ
WQVSRILVYLKMSMDQFIAGKLSANQILLIFGHLSTQLPSLHNYNKHLLRDSLHETYI
RIVNVKNPEKKNVLIECLIVQIAFINNPHHLIGWLNICLQLINTHNKKLLQQLWEMVS
SLESSLAIDWWYTTVLSSQSSKL"
gene complement(<639772..>640371)
/gene="PAC10"
/locus_tag="YGR078C"
/gene_synonym="GIM2; PFD3; RKS2"
/db_xref="GeneID:852969"
mRNA complement(<639772..>640371)
/gene="PAC10"
/locus_tag="YGR078C"
/gene_synonym="GIM2; PFD3; RKS2"
/product="tubulin-binding prefolding complex subunit
PAC10"
/transcript_id="NM_001181207.3"
/db_xref="GeneID:852969"
CDS complement(639772..640371)
/gene="PAC10"
/locus_tag="YGR078C"
/gene_synonym="GIM2; PFD3; RKS2"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:9463374]"
/experiment="EXISTENCE:direct assay:GO:0015631 tubulin
binding [PMID:9463374]"
/experiment="EXISTENCE:mutant phenotype:GO:0007021 tubulin
complex assembly [PMID:9611197]"
/experiment="EXISTENCE:physical interaction:GO:0016272
prefoldin complex [PMID:9463374]"
/note="Part of the heteromeric co-chaperone GimC/prefoldin
complex; complex promotes efficient protein folding"
/codon_start=1
/product="tubulin-binding prefolding complex subunit
PAC10"
/protein_id="NP_011592.3"
/db_xref="GeneID:852969"
/db_xref="SGD:S000003310"
/translation="MDTLFNSTEKNARGIPQAPFIENVNEIIKDPSDFELCFNKFQER
LSKYKFMQESKLATIKQLKTRIPDLENTLKICQSLRNHSDEGDESDEPILLHYQLNDT
LYTKAQVDIPEDRADLKVGLWLGADVMLEYPIDEAIELLKKKLADSEQSLTVSTEDVE
FLRENITTMEVNCARLYNWDVQRRQDLKQAQEGTKNLKI"
gene <640720..>641832
/locus_tag="YGR079W"
/db_xref="GeneID:852970"
mRNA <640720..>641832
/locus_tag="YGR079W"
/product="uncharacterized protein"
/transcript_id="NM_001181208.1"
/db_xref="GeneID:852970"
CDS 640720..641832
/locus_tag="YGR079W"
/note="hypothetical protein; YGR079W is not an essential
gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011593.1"
/db_xref="GeneID:852970"
/db_xref="SGD:S000003311"
/translation="MSSAANEGCVYLFIVVLRLSSFSCVNSFIHSFTRSRTRSSYSLD
ERSLVSYSIVYVAMNKSSKAFQVPNKVITKEDITPLSRSHTKKADTRGTADGKNTTAS
AVEATPIIITTARSIDTAGSLSENATEDDGTQNGDLHDDDDDDDLESTLGYSSEPDPL
FSPCHQPSFTNSTFSYSADNELPMEENHNKNNFHDSSESSIFLPQIQQSFFFGDNSKS
DANNTDFWKEVNGTAEEAICLQETRQRKCSLVALHPGDATTSSNDTLGIEDFIKDDIN
SAEAMEPSPSSSPSSSLLDNLDYNIKLLCYRDNEGKFTLKKRKFLKNSLRSSSAISKK
WKPLSKRDKLLKRAIRRKSGVCQTLSAGFGIGEFML"
gene <642010..>643008
/gene="TWF1"
/locus_tag="YGR080W"
/db_xref="GeneID:852971"
mRNA <642010..>643008
/gene="TWF1"
/locus_tag="YGR080W"
/product="twinfilin TWF1"
/transcript_id="NM_001181209.3"
/db_xref="GeneID:852971"
CDS 642010..643008
/gene="TWF1"
/locus_tag="YGR080W"
/experiment="EXISTENCE:direct assay:GO:0003785 actin
monomer binding [PMID:9700161]"
/experiment="EXISTENCE:direct assay:GO:0005884 actin
filament [PMID:26458246]"
/experiment="EXISTENCE:direct assay:GO:0007015 actin
filament organization [PMID:16569665]"
/experiment="EXISTENCE:direct assay:GO:0030042 actin
filament depolymerization [PMID:26458246|PMID:16569665]"
/experiment="EXISTENCE:direct assay:GO:0030836 positive
regulation of actin filament depolymerization
[PMID:26458246]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807]"
/experiment="EXISTENCE:direct assay:GO:0051014 actin
filament severing [PMID:16569665]"
/experiment="EXISTENCE:direct assay:GO:0051015 actin
filament binding [PMID:16569665]"
/experiment="EXISTENCE:direct assay:GO:0140311 protein
sequestering activity [PMID:9700161]"
/experiment="EXISTENCE:mutant phenotype:GO:0007015 actin
filament organization [PMID:16569665]"
/experiment="EXISTENCE:mutant phenotype:GO:0044396 actin
cortical patch organization [PMID:16569665]"
/experiment="EXISTENCE:mutant phenotype:GO:0051014 actin
filament severing [PMID:16569665]"
/note="Twinfilin; highly conserved actin
monomer-sequestering protein involved in regulation of the
cortical actin cytoskeleton; coordinates actin filament
severing and monomer sequestering at sites of rapid actin
turnover; composed of two cofilin-like regions, stimulates
actin depolymerization as does the mouse homolog, mTwf1"
/codon_start=1
/product="twinfilin TWF1"
/protein_id="NP_011594.3"
/db_xref="GeneID:852971"
/db_xref="SGD:S000003312"
/translation="MSTQSGIVAEQALLHSLNENLSADGIVIIIAKISPDSTSVHQTQ
VARSFEELVQLASQEREPLYIFYKPEGLDKYFFVSFIPDGSPVRSRMLYASTKNTLAR
QVGSNSLSTEQPLITDAQDLVDLKNFDSARPAGQNKPLTHDEEMQIEINKQQALLRKN
TSVKLVSQDSASPLSLTFRVNSEKPINEILDSEGKNLIIFQIDPSNETIQIVQSDTCP
SVDELYIDLPGPSYTIFRQGDSSFFIYSCPSGSKVKDRMIYASNKNGFINYLKNDQKI
AFSKVVEIGDFVELDKSLLMATNKEDSLDHGSNPDLPNKSNLKFNKPKGPLRKRRT"
gene complement(<643148..>643780)
/gene="SLX9"
/locus_tag="YGR081C"
/db_xref="GeneID:852972"
mRNA complement(<643148..>643780)
/gene="SLX9"
/locus_tag="YGR081C"
/product="Slx9p"
/transcript_id="NM_001181210.3"
/db_xref="GeneID:852972"
CDS complement(643148..643780)
/gene="SLX9"
/locus_tag="YGR081C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:17018574]"
/experiment="EXISTENCE:direct assay:GO:0030688
preribosome, small subunit precursor [PMID:17018574]"
/experiment="EXISTENCE:direct assay:GO:0051880
G-quadruplex DNA binding [PMID:31067825]"
/experiment="EXISTENCE:genetic interaction:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:17018574]"
/experiment="EXISTENCE:mutant phenotype:GO:0000056
ribosomal small subunit export from nucleus
[PMID:22956913]"
/experiment="EXISTENCE:mutant phenotype:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:17018574]"
/note="Nuclear export receptor for exportin Crm1p;
required for pre-rRNA processing and nuclear export of
pre-40S pre-ribosomal particles; associates with the 90S
pre-ribosome and 43S small ribosomal subunit precursor;
interacts with U3 snoRNA; binds G-quadruplex (G4)
structures in DNA; deletion mutant has synthetic fitness
defect with an sgs1 deletion mutant"
/codon_start=1
/product="Slx9p"
/protein_id="NP_011595.3"
/db_xref="GeneID:852972"
/db_xref="SGD:S000003313"
/translation="MVAKKRNTLRSKVSARNSQNFGPDVANNGILDESYDIESDPRAF
LHQPKETKKEKLLNRQNTFLSNLKGKSTLNDGIAANFDGISKSSIRRRKRKLREELKP
RMQDLLTSLEQEKDLRGIIENSSKDMNNDDDIDMDSKIRFVDTKEMNLKKIEPGSVRI
KKNQPNIRNQKGAKALAANETARFNQVLTNQDFQKNPFGALREVIKLQKQ"
gene <644044..>644595
/gene="TOM20"
/locus_tag="YGR082W"
/gene_synonym="MAS20; MOM19"
/db_xref="GeneID:852973"
mRNA <644044..>644595
/gene="TOM20"
/locus_tag="YGR082W"
/gene_synonym="MAS20; MOM19"
/product="TOM complex receptor protein TOM20"
/transcript_id="NM_001181211.1"
/db_xref="GeneID:852973"
CDS 644044..644595
/gene="TOM20"
/locus_tag="YGR082W"
/gene_synonym="MAS20; MOM19"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane
[PMID:16407407|PMID:16689936]"
/experiment="EXISTENCE:direct assay:GO:0005742
mitochondrial outer membrane translocase complex
[PMID:9774667]"
/experiment="EXISTENCE:direct assay:GO:0030943
mitochondrion targeting sequence binding [PMID:9252394]"
/experiment="EXISTENCE:mutant phenotype:GO:0008320 protein
transmembrane transporter activity [PMID:8132642]"
/experiment="EXISTENCE:mutant phenotype:GO:0016031 tRNA
import into mitochondrion [PMID:7837265]"
/experiment="EXISTENCE:mutant phenotype:GO:0030150 protein
import into mitochondrial matrix
[PMID:8223428|PMID:8132642|PMID:9774667]"
/note="Component of the TOM (translocase of outer
membrane) complex; responsible for recognition and initial
import steps for all mitochondrially directed proteins;
acts as a receptor for incoming precursor proteins"
/codon_start=1
/product="TOM complex receptor protein TOM20"
/protein_id="NP_011596.1"
/db_xref="GeneID:852973"
/db_xref="SGD:S000003314"
/translation="MSQSNPILRGLAITTAIAALSATGYAIYFDYQRRNSPQFRKVLR
QRAKEQAKMEEQAKTHAKEVKLQKVTEFLSMELAKDPIPSDPSEREATFTTNVENGER
LSMQQGKELEAASKFYKALTVYPQPADLLGIYQRSIPEAIYEYIILMIAILPPANVAS
FVKGVVGSKAESDAVAEANDIDD"
gene complement(<644860..>646815)
/gene="GCD2"
/locus_tag="YGR083C"
/gene_synonym="GCD12"
/db_xref="GeneID:852974"
mRNA complement(<644860..>646815)
/gene="GCD2"
/locus_tag="YGR083C"
/gene_synonym="GCD12"
/product="translation initiation factor eIF2B subunit
delta"
/transcript_id="NM_001181212.1"
/db_xref="GeneID:852974"
CDS complement(644860..646815)
/gene="GCD2"
/locus_tag="YGR083C"
/gene_synonym="GCD12"
/experiment="EXISTENCE:direct assay:GO:0005085
guanyl-nucleotide exchange factor activity [PMID:8506384]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005851 eukaryotic
translation initiation factor 2B complex [PMID:8506384]"
/experiment="EXISTENCE:direct assay:GO:0006446 regulation
of translational initiation [PMID:8506384]"
/experiment="EXISTENCE:direct assay:GO:0030234 enzyme
regulator activity [PMID:8887689]"
/experiment="EXISTENCE:direct assay:GO:0032045
guanyl-nucleotide exchange factor complex [PMID:8506384]"
/experiment="EXISTENCE:genetic interaction:GO:0003743
translation initiation factor activity [PMID:8336705]"
/experiment="EXISTENCE:genetic interaction:GO:0005851
eukaryotic translation initiation factor 2B complex
[PMID:8336705]"
/experiment="EXISTENCE:genetic interaction:GO:0006446
regulation of translational initiation
[PMID:8887689|PMID:8164676]"
/experiment="EXISTENCE:genetic interaction:GO:0030234
enzyme regulator activity [PMID:8887689|PMID:8164676]"
/experiment="EXISTENCE:genetic interaction:GO:0032045
guanyl-nucleotide exchange factor complex [PMID:8336705]"
/experiment="EXISTENCE:mutant phenotype:GO:0003743
translation initiation factor activity [PMID:2038326]"
/experiment="EXISTENCE:mutant phenotype:GO:0006446
regulation of translational initiation [PMID:9032257]"
/experiment="EXISTENCE:mutant phenotype:GO:0030234 enzyme
regulator activity [PMID:9032257]"
/experiment="EXISTENCE:physical interaction:GO:0005851
eukaryotic translation initiation factor 2B complex
[PMID:8506384]"
/experiment="EXISTENCE:physical interaction:GO:0006446
regulation of translational initiation [PMID:8887689]"
/note="Delta subunit of the translation initiation factor
eIF2B; the guanine-nucleotide exchange factor for eIF2;
activity subsequently regulated by phosphorylated eIF2;
first identified as a negative regulator of GCN4
expression"
/codon_start=1
/product="translation initiation factor eIF2B subunit
delta"
/protein_id="NP_011597.1"
/db_xref="GeneID:852974"
/db_xref="SGD:S000003315"
/translation="MSESEAKSRSATPPSKAKQATPTTTAAANGEKKLTNKELKELKK
QEKAAKRAAMKQANGISIEQQQQQAQMKKEKKQLQREQQQKREQKQKNANKKKQNERN
VKKSTLFGHLETTEERRATILALTSAVSSPKTSRITAAGLMVPVVASALSGSNVLTAS
SLMPVGPNASSTVSASAPASTTTTLPASSAALSAGTSSASTNTPTAIQQEIASSNASD
VAKTLASISLEAGEFNVIPGISSVIPTVLEQSFDNSSLISSVKELLLNKDLIHPSILL
LTSHLAHYKIVGSIPRCIAMLEVFQIVIKDYQTPKGTTLSRNLTSYLSHQIDLLKKAR
PLSVTMGNAIRWLKQEISLIDPSTPDKAAKKDLCEKIGQFAKEKIELADQLIIDNAST
QIEESTTIVTYGSSKVLTELLLHNAISLKKNIKVIVVDSRPLFEGRKMAETLRNAGVN
VMYALITSLDTIFNMDVDYVFLGAHSILSNGFLYSRAGTAMLAMSAKRRNIPVLVCCE
SLKFSQRVQLDSVTFNELADPNDLVNIDYENPVERRGNKGALLNQFIKERKFEKKKLA
MENKPKGNKIGGKKGSEGESKDASNEEDSNSKNILDGWQELPSLNIVNILYDLTPPEY
IKKVITEFGALPPSSVPVILREYKGSA"
gene complement(<647123..>648142)
/gene="MRP13"
/locus_tag="YGR084C"
/gene_synonym="mS44"
/db_xref="GeneID:852975"
mRNA complement(<647123..>648142)
/gene="MRP13"
/locus_tag="YGR084C"
/gene_synonym="mS44"
/product="mitochondrial 37S ribosomal protein mS44 MRP13"
/transcript_id="NM_001181213.3"
/db_xref="GeneID:852975"
CDS complement(647123..648142)
/gene="MRP13"
/locus_tag="YGR084C"
/gene_synonym="mS44"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:12392552|PMID:9151978]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:12392552|PMID:9151978]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005763
mitochondrial small ribosomal subunit
[PMID:9151978|PMID:12392552]"
/note="Mitochondrial ribosomal protein of the small
subunit"
/codon_start=1
/product="mitochondrial 37S ribosomal protein mS44 MRP13"
/protein_id="NP_011598.3"
/db_xref="GeneID:852975"
/db_xref="SGD:S000003316"
/translation="MGTITVVINEGPILLIRALHRATTNKKMFRSTVWRRFASTGEIA
KAKLDEFLIYHKTDAKLKPFIYRPKNAQILLTKDIRDPKTREPLQPRPPVKPLSKQTL
NDFIYSVEPNSTELLDWFKEWTGTSIRKRAIWTYISPIHVQKMLTASFFKIGKYAHMV
GLLYGIEHKFLKAQNPSVFDIEHFFNTNIMCALHRNRLKDYKDAEIAQRKLQVAWKKV
LNRKNNTGLANILVATLGRQIGFTPELTGLQPVDISLPDIPNSSSGAELKDLLSKYEG
IYLIARTLLDIDQHNAQYLELQEFIRQYQNALSESSDPYDTHLKALGLLETPPPQEST
EKEEK"
gene complement(<648383..>648907)
/gene="RPL11B"
/locus_tag="YGR085C"
/db_xref="GeneID:852976"
mRNA complement(<648383..>648907)
/gene="RPL11B"
/locus_tag="YGR085C"
/product="60S ribosomal protein uL5 RPL11B"
/transcript_id="NM_001181214.1"
/db_xref="GeneID:852976"
CDS complement(648383..648907)
/gene="RPL11B"
/locus_tag="YGR085C"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:11983894]"
/experiment="EXISTENCE:curator inference:GO:0003735
structural constituent of ribosome [PMID:11983894]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:11983894]"
/experiment="EXISTENCE:mutant phenotype:GO:0000027
ribosomal large subunit assembly [PMID:2277060]"
/note="Ribosomal 60S subunit protein L11B; expressed at
half the level of Rpl11Ap; involved in ribosomal assembly;
depletion causes degradation of 60S proteins and RNA;
homologous to mammalian ribosomal protein L11 and
bacterial L5; RPL11B has a paralog, RPL11A, that arose
from the whole genome duplication"
/codon_start=1
/product="60S ribosomal protein uL5 RPL11B"
/protein_id="NP_011599.1"
/db_xref="GeneID:852976"
/db_xref="SGD:S000003317"
/translation="MSTKAQNPMRDLKIEKLVLNISVGESGDRLTRASKVLEQLSGQT
PVQSKARYTVRTFGIRRNEKIAVHVTVRGPKAEEILERGLKVKEYQLRDRNFSATGNF
GFGIDEHIDLGIKYDPSIGIFGMDFYVVMNRPGARVTRRKRCKGTVGNSHKTTKEDTV
SWFKQKYDADVLDK"
gene complement(<649598..>650617)
/gene="PIL1"
/locus_tag="YGR086C"
/db_xref="GeneID:852977"
mRNA complement(<649598..>650617)
/gene="PIL1"
/locus_tag="YGR086C"
/product="lipid-binding protein PIL1"
/transcript_id="NM_001181215.3"
/db_xref="GeneID:852977"
CDS complement(649598..650617)
/gene="PIL1"
/locus_tag="YGR086C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane
[PMID:36162502|PMID:16407407]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0008289 lipid
binding [PMID:22123866]"
/experiment="EXISTENCE:direct assay:GO:0032126 eisosome
[PMID:16496001|PMID:22869600]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0006897
endocytosis [PMID:16496001]"
/experiment="EXISTENCE:mutant phenotype:GO:0008104
intracellular protein localization [PMID:16496001]"
/experiment="EXISTENCE:mutant phenotype:GO:0070941
eisosome assembly [PMID:19037108|PMID:18296441]"
/experiment="EXISTENCE:mutant phenotype:GO:0097446 protein
localization to eisosome filament [PMID:25811606]"
/note="Eisosome core component involved in endocytosis;
mitochondria phosphoprotein that localizes to the outer
membrane and is involved in maintaining mitochondrial
morphology and mitophagy; phosphorylated (T233) upon Pkc1p
hyperactivation in a Slt2p MAPK-dependent fashion; null
mutant shows activation of Pkc1p/Ypk1p stress resistance
pathways; BAR domain family member; protein increases in
abundance and relocalizes from the plasma membrane to
cytoplasm upon DNA replication stress"
/codon_start=1
/product="lipid-binding protein PIL1"
/protein_id="NP_011600.3"
/db_xref="GeneID:852977"
/db_xref="SGD:S000003318"
/translation="MHRTYSLRNSRAPTASQLQNPPPPPSTTKGRFFGKGGLAYSFRR
SAAGAFGPELSRKLSQLVKIEKNVLRSMELTANERRDAAKQLSIWGLENDDDVSDITD
KLGVLIYEVSELDDQFIDRYDQYRLTLKSIRDIEGSVQPSRDRKDKITDKIAYLKYKD
PQSPKIEVLEQELVRAEAESLVAEAQLSNITRSKLRAAFNYQFDSIIEHSEKIALIAG
YGKALLELLDDSPVTPGETRPAYDGYEASKQIIIDAESALNEWTLDSAQVKPTLSFKQ
DYEDFEPEEGEEEEEEDGQGRWSEDEQEDGQIEEPEQEEEGAVEEHEQVGHQQSESLP
QQTTA"
gene 650855..651159
/gene="SUT125"
/locus_tag="YNCG0048W"
/db_xref="GeneID:84313895"
ncRNA 650855..651159
/ncRNA_class="other"
/gene="SUT125"
/locus_tag="YNCG0048W"
/product="SUT125"
/note="Stable Unannotated Transcript functional ncRNA;
regulates gene expression in trans by modulating
transcription factors; improves endogenous invertase
secretion when deleted"
/transcript_id="NR_188206.1"
/db_xref="GeneID:84313895"
/db_xref="SGD:S000345041"
gene complement(<651290..>652981)
/gene="PDC6"
/locus_tag="YGR087C"
/db_xref="GeneID:852978"
mRNA complement(<651290..>652981)
/gene="PDC6"
/locus_tag="YGR087C"
/product="indolepyruvate decarboxylase 6"
/transcript_id="NM_001181216.3"
/db_xref="GeneID:852978"
CDS complement(651290..652981)
/gene="PDC6"
/locus_tag="YGR087C"
/EC_number="4.1.1.43"
/EC_number="4.1.1.72"
/EC_number="4.1.1.74"
/experiment="EXISTENCE:direct assay:GO:0004737 pyruvate
decarboxylase activity [PMID:23423327]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0000949
aromatic amino acid family catabolic process to alcohol
via Ehrlich pathway [PMID:12499363]"
/experiment="EXISTENCE:genetic interaction:GO:0006559
L-phenylalanine catabolic process [PMID:12499363]"
/experiment="EXISTENCE:genetic interaction:GO:0006569
L-tryptophan catabolic process [PMID:12499363]"
/experiment="EXISTENCE:mutant phenotype:GO:0004737
pyruvate decarboxylase activity [PMID:1744053]"
/experiment="EXISTENCE:mutant phenotype:GO:0006067 ethanol
metabolic process [PMID:1744053]"
/note="Minor isoform of pyruvate decarboxylase;
decarboxylates pyruvate to acetaldehyde, involved in amino
acid catabolism; transcription is glucose- and
ethanol-dependent, and is strongly induced during sulfur
limitation"
/codon_start=1
/product="indolepyruvate decarboxylase 6"
/protein_id="NP_011601.3"
/db_xref="GeneID:852978"
/db_xref="SGD:S000003319"
/translation="MSEITLGKYLFERLKQVNVNTIFGLPGDFNLSLLDKIYEVDGLR
WAGNANELNAAYAADGYARIKGLSVLVTTFGVGELSALNGIAGSYAEHVGVLHVVGVP
SISAQAKQLLLHHTLGNGDFTVFHRMSANISETTSMITDIATAPSEIDRLIRTTFITQ
RPSYLGLPANLVDLKVPGSLLEKPIDLSLKPNDPEAEKEVIDTVLELIQNSKNPVILS
DACASRHNVKKETQKLIDLTQFPAFVTPLGKGSIDEQHPRYGGVYVGTLSKQDVKQAV
ESADLILSVGALLSDFNTGSFSYSYKTKNVVEFHSDYVKVKNATFLGVQMKFALQNLL
KVIPDVVKGYKSVPVPTKTPANKGVPASTPLKQEWLWNELSKFLQEGDVIISETGTSA
FGINQTIFPKDAYGISQVLWGSIGFTTGATLGAAFAAEEIDPNKRVILFIGDGSLQLT
VQEISTMIRWGLKPYLFVLNNDGYTIEKLIHGPHAEYNEIQTWDHLALLPAFGAKKYE
NHKIATTGEWDALTTDSEFQKNSVIRLIELKLPVFDAPESLIKQAQLTAATNAKQ"
gene <654634..>656322
/gene="CTT1"
/locus_tag="YGR088W"
/gene_synonym="SPS101"
/db_xref="GeneID:852979"
mRNA <654634..>656322
/gene="CTT1"
/locus_tag="YGR088W"
/gene_synonym="SPS101"
/product="catalase T"
/transcript_id="NM_001181217.1"
/db_xref="GeneID:852979"
CDS 654634..656322
/gene="CTT1"
/locus_tag="YGR088W"
/gene_synonym="SPS101"
/EC_number="1.11.1.6"
/experiment="EXISTENCE:direct assay:GO:0004096 catalase
activity [PMID:19463791]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0000302
response to reactive oxygen species [PMID:15893481]"
/note="Cytosolic catalase T; has a role in protection from
oxidative damage by hydrogen peroxide"
/codon_start=1
/product="catalase T"
/protein_id="NP_011602.2"
/db_xref="GeneID:852979"
/db_xref="SGD:S000003320"
/translation="MNVFGKKEEKQEKVYSLQNGFPYSHHPYASQYSRPDGPILLQDF
HLLENIASFDRERVPERVVHAKGGGCRLEFELTDSLSDITYAAPYQNVGYKCPGLVRF
STVGGESGTPDTARDPRGVSFKFYTEWGNHDWVFNNTPVFFLRDAIKFPVFIHSQKRD
PQSHLNQFQDTTIYWDYLTLNPESIHQITYMFGDRGTPASWASMNAYSGHSFIMVNKE
GKDTYVQFHVLSDTGFETLTGDKAAELSGSHPDYNQAKLFTQLQNGEKPKFNCYVQTM
TPEQATKFRYSVNDLTKIWPHKEFPLRKFGTITLTENVDNYFQEIEQVAFSPTNTCIP
GIKPSNDSVLQARLFSYPDTQRHRLGANYQQLPVNRPRNLGCPYSKGDSQYTAEQCPF
KAVNFQRDGPMSYYNFGPEPNYISSLPNQTLKFKNEDNDEVSDKFKGIVLDEVTEVSV
RKQEQDQIRNEHIVDAKINQYYYVYGISPLDFEQPRALYEKVYNDEQKKLFVHNVVCH
ACKIKDPKVKKRVTQYFGLLNEDLGKVIAECLGVPWEPVDLEGYAKTWSIASAN"
gene <656960..>659770
/gene="NNF2"
/locus_tag="YGR089W"
/db_xref="GeneID:852980"
mRNA <656960..>659770
/gene="NNF2"
/locus_tag="YGR089W"
/product="Nnf2p"
/transcript_id="NM_001181218.1"
/db_xref="GeneID:852980"
CDS 656960..659770
/gene="NNF2"
/locus_tag="YGR089W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/note="Protein that exhibits physical and genetic
interactions with Rpb8p; Rpb8p is a subunit of RNA
polymerases I, II, and III; computational analysis of
large-scale protein-protein interaction data suggests a
role in chromosome segregation"
/codon_start=1
/product="Nnf2p"
/protein_id="NP_011603.1"
/db_xref="GeneID:852980"
/db_xref="SGD:S000003321"
/translation="MEEQFTNQKKVSHLQSLMNTKRSEQPTEFAKKHRFKDTLALFLV
FLSFNHFTSLCLLVSFIVATKCKDFLANCFIILFLSKKPSRHIGEVAHIDISTSKVTN
GSSNRKSNSRFFGNSKNSFVIPIPVLICEILFAMLLKIYGGDYFVKPIKNLAISIIAS
FLINDPSDCLSYATSCSVLYAVSTNTFQRVSHFFDIIQLFDMSLRGTGQSIKLFTVFR
KYSQFFKKLFSLFLPMPFKMLGKHSDSMIYYLSFHILFFSFASSLLHPHRQTAENKPL
KKGFNSTKPDVARVQGLQKMRISSSSSVSADSNTLEDQSPMIPNDPGGLSSSNQTIHP
SQQNNSPVPLSSHSNILNPAASYPTDATSSFPYFTSMVKEYKSYQPSVISAEGSNSQA
VTTTTSTTTSPTTFNFSGDNTSLSNEISLSDSSNGNSKKDSDFFSPSNDKYTNQLFEL
NVDFGNMFSSSKLSSDISVTSNLENFIRLLFRRKNQHLIAPLWSMVVTLKTTNFEKKY
LQETSENSLTPTNSNTSYVSNQEKHDKDLDTINTHSVSSRISFTHAGKFKKSVFNNFE
PSNTMALIAKTTSDDYNLLNLVSTNENIFNRNDNDYKVCIIDISTNSITFHIENLHDG
ELIVLVNGVIWSEVSCALILEHVGEEYVVVNGLVPSCSYDIQFINRLNHRDDYLVSDL
IVRTCGNNNAIAGKFENLDFSFPSYYHRKFLSPLLTLKHSVLTTNANLSDERTKLKKT
KKEFSKKLSLLRQEIDYFKGRISQNATHDEKSTLKVENLKVALQQSETAVNKLEMQLK
TLTEKELELEEEYLKKKDLHLKNQLEFSKLEESLSKDLKNSEGRFQKVNQELVQLGSK
LDKLNARNEKLQKEVDQNAEEIEKFSTQFLSKREKDRFRRKEYRIREANKFELTIKGL
EQDINRLENENENIHSLIGNSY"
rep_origin 659907..660002
/note="ARS727; Autonomously Replicating Sequence"
/db_xref="SGD:S000118463"
gene 660087..661399
/gene="SUT126"
/locus_tag="YNCG0049W"
/db_xref="GeneID:84313896"
ncRNA 660087..661399
/ncRNA_class="other"
/gene="SUT126"
/locus_tag="YNCG0049W"
/product="SUT126"
/note="Stable Unannotated Transcript functional ncRNA;
acts in trans to regulate gene expression by modulating
transcription factors"
/transcript_id="NR_188207.1"
/db_xref="GeneID:84313896"
/db_xref="SGD:S000345042"
gene 661749..661820
/locus_tag="YNCG0027W"
/db_xref="GeneID:852981"
tRNA 661749..661820
/locus_tag="YNCG0027W"
/product="tRNA-Thr"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0030371 translation
repressor activity [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043555 regulation
of translation in response to stress [PMID:27609601]"
/note="Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:852981"
/db_xref="SGD:S000006749"
gene <662358..>666071
/gene="UTP22"
/locus_tag="YGR090W"
/db_xref="GeneID:852982"
mRNA <662358..>666071
/gene="UTP22"
/locus_tag="YGR090W"
/product="rRNA-processing protein UTP22"
/transcript_id="NM_001181219.3"
/db_xref="GeneID:852982"
CDS 662358..666071
/gene="UTP22"
/locus_tag="YGR090W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:14562095|PMID:15590835|PMID:12150911]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/experiment="EXISTENCE:direct assay:GO:0032040
small-subunit processome [PMID:15590835]"
/experiment="EXISTENCE:direct assay:GO:0032545 CURI
complex [PMID:17452446]"
/experiment="EXISTENCE:direct assay:GO:0034456 UTP-C
complex [PMID:17515605]"
/experiment="EXISTENCE:mutant phenotype:GO:0006364 rRNA
processing [PMID:12837249]"
/experiment="EXISTENCE:mutant phenotype:GO:0006409 tRNA
export from nucleus [PMID:23194188]"
/note="Component of the small-subunit processome; required
for nuclear export of tRNAs; forms a complex with Rrp7p;
may facilitate binding of Utp8p to aminoacylated tRNAs and
their delivery to Los1p for export; conserved from yeast
to mammals"
/codon_start=1
/product="rRNA-processing protein UTP22"
/protein_id="NP_011604.3"
/db_xref="GeneID:852982"
/db_xref="SGD:S000003322"
/translation="MATSVKRKASETSDQNIVKVQKKHSTQDSTTDNGSKENDHSSQA
INERTVPEQENDESDTSPESNEVATNTAATRHNGKVTATESYDIHIARETAELFKSNI
FKLQIDELLEQVKLKQKHVLKVEKFLHKLYDILQEIPDWEEKSLAEVDSFFKNKIVSV
PFVDPKPIPQNTNYKFNYKKPDISLIGSFALKAGIYQPNGSSIDTLLTMPKELFEKKD
FLNFRCLHKRSVYLAYLTHHLLILLKKDKLDSFLQLEYSYFDNDPLLPILRISCSKPT
GDSLSDYNFYKTRFSINLLIGFPYKVFEPKKLLPNRNCIRIAQESKEQSLPATPLYNF
SVLSSSTHENYLKYLYKTKKQTESFVEATVLGRLWLQQRGFSSNMSHSGSLGGFGTFE
FTILMAALLNGGGINSNKILLHGFSSYQLFKGVIKYLATMDLCHDGHLQFHSNPENSS
SSPASKYIDEGFQTPTLFDKSTKVNILTKMTVSSYQILKEYAGETLRMLNNVVQDQFS
NIFLTNISRFDNLKYDLCYDVQLPLGKYNNLETSLAATFGSMERVKFITLENFLAHKI
TNVARYALGDRIKYIQIEMVGQKSDFPITKRKVYSNTGGNHFNFDFVRVKLIVNPSEC
DKLVTKGPAHSETMSTEAAVFKNFWGIKSSLRRFKDGSITHCCVWSTSSSEPIISSIV
NFALQKHVSKKAQISNETIKKFHNFLPLPNLPSSAKTSVLNLSSFFNLKKSFDDLYKI
IFQMKLPLSVKSILPVGSAFRYTSLCQPVPFAYSDPDFFQDVILEFETSPKWPDEITS
LEKAKTAFLLKIQEELSANSSTYRSFFSRDESIPYNLEIVTLNILTPEGYGFKFRVLT
ERDEILYLRAIANARNELKPELEATFLKFTAKYLASVRHTRTLENISHSYQFYSPVVR
LFKRWLDTHLLLGHITDELAELIAIKPFVDPAPYFIPGSLENGFLKVLKFISQWNWKD
DPLILDLVKPEDDIRDTFETSIGAGSELDSKTMKKLSERLTLAQYKGIQMNFTNLRNS
DPNGTHLQFFVASKNDPSGILYSSGIPLPIATRLTALAKVAVNLLQTHGLNQQTINLL
FTPGLKDYDFVVDLRTPIGLKSSCGILSATEFKNITNDQAPSNFPENLNDLSEKMDPT
YQLVKYLNLKYKNSLILSSRKYIGVNGGEKGDKNVITGLIKPLFKGAHKFRVNLDCNV
KPVDDENVILNKEAIFHEIAAFGNDMVINFETD"
gene <666341..>667825
/gene="PRP31"
/locus_tag="YGR091W"
/db_xref="GeneID:852983"
mRNA <666341..>667825
/gene="PRP31"
/locus_tag="YGR091W"
/product="U4/U6-U5 snRNP complex subunit PRP31"
/transcript_id="NM_001181220.1"
/db_xref="GeneID:852983"
CDS 666341..667825
/gene="PRP31"
/locus_tag="YGR091W"
/experiment="EXISTENCE:direct assay:GO:0000387
spliceosomal snRNP assembly [PMID:9199293]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0046540 U4/U6 x U5
tri-snRNP complex [PMID:10377396|PMID:10449419]"
/experiment="EXISTENCE:mutant phenotype:GO:0000387
spliceosomal snRNP assembly [PMID:9199293]"
/note="Splicing factor; component of the U4/U6-U5 snRNP
complex; homologous to human PRPF31 and used to study
retinitis pigmentosa"
/codon_start=1
/product="U4/U6-U5 snRNP complex subunit PRP31"
/protein_id="NP_011605.1"
/db_xref="GeneID:852983"
/db_xref="SGD:S000003323"
/translation="MSSEEDYFDELEYDLADEVNEEKEDIQTKKLTTVNCQTEKFNPF
EILPESIELFRTLALISPDRLSLSETAQILPKIVDLKRILQQQEIDFIKLLPFFNEII
PLIKSNIKLMHNFLISLYSRRFPELSSLIPSPLQYSKVISILENENYSKNESDELFFH
LENKAKLTREQILVLTMSMKTSFKNKEPLDIKTRTQILEANSILENLWKLQEDIGQYI
ASKISIIAPNVCFLVGPEIAAQLIAHAGGVLEFSRIPSCNIASIGKNKHLSHELHTLE
SGVRQEGYLFASDMIQKFPVSVHKQMLRMLCAKVSLAARVDAGQKNGDRNTVLAHKWK
AELSKKARKLSEAPSISETKALPIPEDQPKKKRAGRKFRKYKEKFRLSHVRQLQNRME
FGKQEQTVLDSYGEEVGLGMSNTSLQQAVGATSGSRRSAGNQAKLTKVMKHRISEANQ
QADEFLISLGHNTEQPNLSPEMVQMHKKQHTNPEEETNWFSGHG"
gene <668189..>669907
/gene="DBF2"
/locus_tag="YGR092W"
/db_xref="GeneID:852984"
mRNA <668189..>669907
/gene="DBF2"
/locus_tag="YGR092W"
/product="serine/threonine-protein kinase DBF2"
/transcript_id="NM_001181221.3"
/db_xref="GeneID:852984"
CDS 668189..669907
/gene="DBF2"
/locus_tag="YGR092W"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0004674 protein
serine/threonine kinase activity
[PMID:8131744|PMID:21498574]"
/experiment="EXISTENCE:direct assay:GO:0005816 spindle
pole body [PMID:10984431|PMID:11434459]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:11434459|PMID:10984431]"
/experiment="EXISTENCE:direct assay:GO:0034973 Sid2-Mob1
complex [PMID:9528782]"
/experiment="EXISTENCE:mutant phenotype:GO:0000280 nuclear
division [PMID:2181271]"
/experiment="EXISTENCE:mutant phenotype:GO:0000910
cytokinesis [PMID:22573892]"
/experiment="EXISTENCE:mutant phenotype:GO:0004674 protein
serine/threonine kinase activity [PMID:22573892]"
/experiment="EXISTENCE:mutant phenotype:GO:0007035
vacuolar acidification [PMID:18048916]"
/experiment="EXISTENCE:mutant phenotype:GO:0032465
regulation of cytokinesis [PMID:22573892]"
/experiment="EXISTENCE:mutant phenotype:GO:1901900
regulation of protein localization to cell division site
[PMID:22573892]"
/experiment="EXISTENCE:physical interaction:GO:0034973
Sid2-Mob1 complex [PMID:9528782]"
/note="Ser/Thr kinase involved in transcription and stress
response; functions as part of a network of genes in exit
from mitosis; localization is cell cycle regulated;
activated by Cdc15p during the exit from mitosis; also
plays a role in regulating the stability of SWI5 and CLB2
mRNAs; phosphorylates Chs2p to regulate primary septum
formation and Hof1p to regulate cytokinesis; DBF2 has a
paralog, DBF20, that arose from the whole genome
duplication"
/codon_start=1
/product="serine/threonine-protein kinase DBF2"
/protein_id="NP_011606.3"
/db_xref="GeneID:852984"
/db_xref="SGD:S000003324"
/translation="MLSKSEKNVDLLAGNMSNLSFDGHGTPGGTGLFPNQNITKRRTR
PAGINDSPSPVKPSFFPYEDTSNMDIDEVSQPDMDVSNSPKKLPPKFYERATSNKTQR
VVSVCKMYFLEHYCDMFDYVISRRQRTKQVLEYLQQQSQLPNSDQIKLNEEWSSYLQR
EHQVLRKRRLKPKNRDFEMITQVGQGGYGQVYLARKKDTKEVCALKILNKKLLFKLNE
TKHVLTERDILTTTRSEWLVKLLYAFQDLQSLYLAMEFVPGGDFRTLLINTRCLKSGH
ARFYISEMFCAVNALHDLGYTHRDLKPENFLIDAKGHIKLTDFGLAAGTISNERIESM
KIRLEKIKDLEFPAFTEKSIEDRRKMYNQLREKEINYANSMVGSPDYMALEVLEGKKY
DFTVDYWSLGCMLFESLVGYTPFSGSSTNETYDNLRRWKQTLRRPRQSDGRAAFSDRT
WDLITRLIADPINRLRSFEHVKRMSYFADINFSTLRSMIPPFTPQLDSETDAGYFDDF
TSEADMAKYADVFKRQDKLTAMVDDSAVSSKLVGFTFRHRNGKQGSSGILFNGLEHSD
PFSTFY"
gene <670388..>671911
/gene="DRN1"
/locus_tag="YGR093W"
/db_xref="GeneID:852985"
mRNA <670388..>671911
/gene="DRN1"
/locus_tag="YGR093W"
/product="Drn1p"
/transcript_id="NM_001181222.3"
/db_xref="GeneID:852985"
CDS 670388..671911
/gene="DRN1"
/locus_tag="YGR093W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0008047 enzyme
activator activity [PMID:24919400]"
/experiment="EXISTENCE:direct assay:GO:0061632 RNA lariat
debranching enzyme activator activity [PMID:24919400]"
/experiment="EXISTENCE:mutant phenotype:GO:0000398 mRNA
splicing, via spliceosome [PMID:24919400]"
/note="Splicing factor that modulates turnover of branched
RNAs by Dbr1p; interacts with spliceosomal components and
branched RNA splicing products; enhances Dbr1p debranching
in vitro; conserved protein with domain organization
identical from yeast to human; N-terminal homology to
Dbr1p N-terminus, but Dbr1p catalytic residues not
conserved; relocalizes to the cytosol in response to
hypoxia"
/codon_start=1
/product="Drn1p"
/protein_id="NP_011607.3"
/db_xref="GeneID:852985"
/db_xref="SGD:S000003325"
/translation="MTNAKILVAHISESDADEAIRKIKKVNEKSGPFDLIIIFSNSYD
ENFELNTDGLPQLILLSCDKANNSKSKKINENVTLLHNMGTYKLANGITLSYFIYPDD
TLQGEKKSILDEFGKSEDQVDILLTKEWGLSISERCGRLSGSEVVDELAKKLQARYHF
AFSDEINFYELEPFQWERERLSRFLNIPKYGSGKKWAYAFNMPIGDNELKDEPEPPNL
IANPYNSVVTNSNKRPLETETENSFDGDKQVLANREKNENKKIRTILPSSCHFCFSNP
NLEDHMIISIGKLVYLTTAKGPLSVPKGDMDISGHCLIIPIEHIPKLDPSKNAELTQS
ILAYEASLVKMNYIKFDMCTIVFEIQSERSIHFHKQVIPVPKYLVLKFCSALDRQVHF
NNEKFTRNAKLEFQCYDSHSSKQYVDVINNQSNNYLQFTVYETPEADPKIYLATFNAS
ETIDLQFGRRVLAFLLNLPRRVKWNSSTCLQTKQQETIEAEKFQKAYRTYDISLTEN"
gene <672186..>675500
/gene="VAS1"
/locus_tag="YGR094W"
/db_xref="GeneID:852986"
mRNA <672186..>675500
/gene="VAS1"
/locus_tag="YGR094W"
/product="valine--tRNA ligase"
/transcript_id="NM_001181223.1"
/db_xref="GeneID:852986"
CDS 672186..675500
/gene="VAS1"
/locus_tag="YGR094W"
/EC_number="6.1.1.9"
/experiment="EXISTENCE:direct assay:GO:0004832 valine-tRNA
ligase activity [PMID:782885]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0006438 valyl-tRNA
aminoacylation [PMID:782885]"
/experiment="EXISTENCE:direct assay:GO:1990825
sequence-specific mRNA binding [PMID:34039240]"
/experiment="EXISTENCE:mutant phenotype:GO:0005737
cytoplasm [PMID:3275649]"
/experiment="EXISTENCE:mutant phenotype:GO:0005739
mitochondrion [PMID:3275649]"
/note="Mitochondrial and cytoplasmic valyl-tRNA
synthetase; human homolog VARS2 implicated in
mitochondrial diseases, can partially complement yeast
null mutant"
/codon_start=1
/product="valine--tRNA ligase"
/protein_id="NP_011608.1"
/db_xref="GeneID:852986"
/db_xref="SGD:S000003326"
/translation="MNKWLNTLSKTFTFRLLNCHYRRSLPLCQNFSLKKSLTHNQVRF
FKMSDLDNLPPVDPKTGEVIINPLKEDGSPKTPKEIEKEKKKAEKLLKFAAKQAKKNA
AATTGASQKKPKKKKEVEPIPEFIDKTVPGEKKILVSLDDPALKAYNPANVESSWYDW
WIKTGVFEPEFTADGKVKPEGVFCIPAPPPNVTGALHIGHALTIAIQDSLIRYNRMKG
KTVLFLPGFDHAGIATQSVVEKQIWAKDRKTRHDYGREAFVGKVWEWKEEYHSRIKNQ
IQKLGASYDWSREAFTLSPELTKSVEEAFVRLHDEGVIYRASRLVNWSVKLNTAISNL
EVENKDVKSRTLLSVPGYDEKVEFGVLTSFAYPVIGSDEKLIIATTRPETIFGDTAVA
VHPDDDRYKHLHGKFIQHPFLPRKIPIITDKEAVDMEFGTGAVKITPAHDQNDYNTGK
RHNLEFINILTDDGLLNEECGPEWQGMKRFDARKKVIEQLKEKNLYVGQEDNEMTIPT
CSRSGDIIEPLLKPQWWVSQSEMAKDAIKVVRDGQITITPKSSEAEYFHWLGNIQDWC
ISRQLWWGHRCPVYFINIEGEEHDRIDGDYWVAGRSMEEAEKKAAAKYPNSKFTLEQD
EDVLDTWFSSGLWPFSTLGWPEKTKDMETFYPFSMLETGWDILFFWVTRMILLGLKLT
GSVPFKEVFCHSLVRDAQGRKMSKSLGNVIDPLDVITGIKLDDLHAKLLQGNLDPREV
EKAKIGQKESYPNGIPQCGTDAMRFALCAYTTGGRDINLDILRVEGYRKFCNKIYQAT
KFALMRLGDDYQPPATEGLSGNESLVEKWILHKLTETSKIVNEALDKRDFLTSTSSIY
EFWYLICDVYIENSKYLIQEGSAIEKKSAKDTLYILLDNALKLIHPFMPFISEEMWQR
LPKRSTEKAASIVKASYPVYVSEYDDVKSANAYDLVLNITKEARSLLSEYNILKNGKV
FVESNHEEYFKTAEDQKDSIVSLIKAIDEVTVVRDASEIPEGCVLQSVNPEVNVHLLV
KGHVDIDAEIAKVQKKLEKAKKSKNGIEQTINSKDYETKANTQAKEANKSKLDNTVAE
IEGLEATIENLKRLKL"
gene complement(<675671..>676342)
/gene="RRP46"
/locus_tag="YGR095C"
/db_xref="GeneID:852987"
mRNA complement(<675671..>676342)
/gene="RRP46"
/locus_tag="YGR095C"
/product="exosome non-catalytic core subunit RRP46"
/transcript_id="NM_001181224.1"
/db_xref="GeneID:852987"
CDS complement(675671..676342)
/gene="RRP46"
/locus_tag="YGR095C"
/experiment="EXISTENCE:direct assay:GO:0000176 nuclear
exosome (RNase complex) [PMID:19046973|PMID:10465791]"
/experiment="EXISTENCE:direct assay:GO:0000177 cytoplasmic
exosome (RNase complex) [PMID:19046973|PMID:10465791]"
/experiment="EXISTENCE:direct assay:GO:0071038
TRAMP-dependent tRNA surveillance pathway
[PMID:15828860|PMID:17643380]"
/experiment="EXISTENCE:mutant phenotype:GO:0000467
exonucleolytic trimming to generate mature 3'-end of 5.8S
rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S
rRNA, LSU-rRNA) [PMID:10508172|PMID:10465791]"
/experiment="EXISTENCE:mutant phenotype:GO:0071035 nuclear
polyadenylation-dependent rRNA catabolic process
[PMID:10465791]"
/experiment="EXISTENCE:mutant phenotype:GO:0071042 nuclear
polyadenylation-dependent mRNA catabolic process
[PMID:19369424]"
/note="Exosome non-catalytic core component; involved in
3'-5' RNA processing and degradation in both the nucleus
and the cytoplasm; has similarity to E. coli RNase PH and
to human hRrp46p (EXOSC5)"
/codon_start=1
/product="exosome non-catalytic core subunit RRP46"
/protein_id="NP_011609.2"
/db_xref="GeneID:852987"
/db_xref="SGD:S000003327"
/translation="MSVQAEIGILDHVDGSSEFVSQDTKVICSVTGPIEPKARQELPT
QLALEIIVRPAKGVATTREKVLEDKLRAVLTPLITRHCYPRQLCQITCQILESGEDEA
EFSLRELSCCINAAFLALVDAGIALNSMCASIPIAIIKDTSDIIVDPTAEQLKISLSV
HTLALEFVNGGKVVKNVLLLDSNGDFNEDQLFSLLELGEQKCQELVTNIRRIIQDNIS
PRLVV"
gene <676621..>677565
/gene="TPC1"
/locus_tag="YGR096W"
/db_xref="GeneID:852988"
mRNA <676621..>677565
/gene="TPC1"
/locus_tag="YGR096W"
/product="thiamine transporter TPC1"
/transcript_id="NM_001181225.3"
/db_xref="GeneID:852988"
CDS 676621..677565
/gene="TPC1"
/locus_tag="YGR096W"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:12411483]"
/experiment="EXISTENCE:direct assay:GO:0090422 thiamine
pyrophosphate transmembrane transporter activity
[PMID:12411483]"
/experiment="EXISTENCE:direct assay:GO:1990545
mitochondrial thiamine pyrophosphate transmembrane
transport [PMID:12411483]"
/experiment="EXISTENCE:mutant phenotype:GO:1990545
mitochondrial thiamine pyrophosphate transmembrane
transport [PMID:12411483]"
/note="Mitochondrial membrane transporter; mediates uptake
of the essential cofactor thiamine pyrophosphate (ThPP)
into mitochondria; expression appears to be regulated by
carbon source; member of the mitochondrial carrier family"
/codon_start=1
/product="thiamine transporter TPC1"
/protein_id="NP_011610.3"
/db_xref="GeneID:852988"
/db_xref="SGD:S000003328"
/translation="MFKEEDSLRKGQNVAAWKTLLAGAVSGLLARSITAPMDTIKIRL
QLTPANGLKPFGSQVMEVARSMIKNEGIRSFWKGNIPGSLLYVTYGSAQFSSYSLFNR
YLTPFGLEARLHSLVVGAFAGITSSIVSYPFDVLRTRLVANNQMHSMSITREVRDIWK
LEGLPGFFKGSIASMTTITLTASIMFGTYETIRIYCDENEKTTAAHKKWELATLNHSA
GTIGGVIAKIITFPLETIRRRMQFMNSKHLEKFSRHSSVYGSYKGYGFARIGLQILKQ
EGVSSLYRGILVALSKTIPTTFVSFWGYETAIHYLRMY"
gene <678695..>682135
/gene="ASK10"
/locus_tag="YGR097W"
/gene_synonym="RGC2"
/db_xref="GeneID:852989"
mRNA <678695..>682135
/gene="ASK10"
/locus_tag="YGR097W"
/gene_synonym="RGC2"
/product="Ask10p"
/transcript_id="NM_001181226.1"
/db_xref="GeneID:852989"
CDS 678695..682135
/gene="ASK10"
/locus_tag="YGR097W"
/gene_synonym="RGC2"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:19956799]"
/experiment="EXISTENCE:genetic interaction:GO:0034599
cellular response to oxidative stress [PMID:14555478]"
/experiment="EXISTENCE:mutant phenotype:GO:0016247 channel
regulator activity [PMID:24298058]"
/experiment="EXISTENCE:mutant phenotype:GO:0030163 protein
catabolic process [PMID:14555478]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress [PMID:14555478]"
/experiment="EXISTENCE:mutant phenotype:GO:0090372
positive regulation of glycerol transport [PMID:19956799]"
/note="Regulator of the Fps1p glycerol channel; under
nonstress conditions, binds to Fps1p to positively
regulate glycerol transport; under osmotic stress,
multiple phosphorylation by Hog1p causes Ask10p to
dissociate from Fps1p; forms homodimers and
heterodimerizes with paralog Rgc1p; phosphorylated in
response to oxidative stress; has a role in destruction of
Ssn8p; associates with RNA polymerase II holoenzyme"
/codon_start=1
/product="Ask10p"
/protein_id="NP_011611.1"
/db_xref="GeneID:852989"
/db_xref="SGD:S000003329"
/translation="MSDYFSSRPSQTLTPMGNKPSGGGGGDDASSIHSKSSQYLMDIL
PDSMTLNESVSSIVANNQAKEFILPETDERSPYFINVPIPKAQPTSTTETKKPLAGDE
AIDGQFVKEYPTDILVDRFYKWKKILKGLVIYLREVAYAQEQFARINYQLKGSVKFPF
LTDIDETTNTITDPFTTAPRGPKKAQPAQKKVGLTDSEQFQMQMQQEQQENAVQAPTD
ESKMSLAPHEYKPVQTTESDNTSAASGFVKFGSGSIQDIQVILKKYHLSLANQQFKIS
KEITSTVIPKLEELRKDLRYKITEIKDLHGDFKTNIGAHIQLTSQLLKKYIAAVKFMN
AHGIGNDRASPTNKKPHKLDPKHDPYLLKLQLDLQLKRQVAEETYLQEAFINLQSSGL
QLEKIIYTKIQHALLRYSALIDSEARLMIKNMCQELQHGIISKPPAFEWDNFVTQHPS
CLLNWKSNDPIPPPRKVSDVIYPHMKSPLAKCIKAGYFLKKSELLPTYHQGYFVLTSN
YIHEFQSSDFYNLSSSTPNSTKSSAYSSSVSIADTYANANNAKANNHHRQASDVHNSS
TTTGGTAGANGIRGIRKKSYLAPIMSIPLNDCTLKDASSTKFVLVGKPTLNENADVRK
SSSSTYLSGSSQASLPKYGHETAKIFSKAPFHKFLKGSKPKNKNTKSSELDQFYAAAQ
KESNNYVTWTFKIVSPEPSEEELKHFKRWVQDLKNLTSFNDTKDRIKFIEDRVMKSHR
FKAGHMSRNSVNIGSHTPCLTDSTFTLQDGTTTSVNLKGRAEKPQYIHIQNNSLADFD
GNGFRSKVNTPAIDDYGNLITVERRPAQSPHQYSDYMATSGNTTPSYSSGSRPQSMYN
GYNPAVSITSNGMMLQQSTANNNTNPTTNLRHQRNISQTSSLPGFSYTSLSLPVNSPG
SSNSESSSGGYFAIPLHGNNNNNNYTQRNSEGSSPCYNDDQIRQQQQPLQMQPLSRTS
SSSVNVTAMRSTSAGNSITANAPVVPKVMVNNQNVKTVAADQSATAPSSPTMNSSVTT
INRESPYQTLKKTNSTGNVPCLTAEKTHAHPAFYKRGNNSAQNLTTSSSTASRVHPIR
KHKKNVSFSSLNSLMFSKKGANHGGNLMTNQFMSGGIQEDDGDSTNNDTIKLNQSIYS
"
gene complement(<682566..>687458)
/gene="ESP1"
/locus_tag="YGR098C"
/db_xref="GeneID:852990"
mRNA complement(<682566..>687458)
/gene="ESP1"
/locus_tag="YGR098C"
/product="separase"
/transcript_id="NM_001181227.3"
/db_xref="GeneID:852990"
CDS complement(682566..687458)
/gene="ESP1"
/locus_tag="YGR098C"
/EC_number="3.4.22.49"
/experiment="EXISTENCE:direct assay:GO:0004197
cysteine-type endopeptidase activity
[PMID:11081625|PMID:11081626]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922|PMID:11149918]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922|PMID:11149918]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005819 spindle
[PMID:11149918]"
/experiment="EXISTENCE:direct assay:GO:0007135 meiosis II
[PMID:11081626]"
/experiment="EXISTENCE:genetic interaction:GO:0031536
positive regulation of exit from mitosis [PMID:14551257]"
/experiment="EXISTENCE:genetic interaction:GO:0032888
regulation of mitotic spindle elongation [PMID:18430955]"
/experiment="EXISTENCE:mutant phenotype:GO:0000070 mitotic
sister chromatid segregation [PMID:9635435]"
/experiment="EXISTENCE:mutant phenotype:GO:0004197
cysteine-type endopeptidase activity [PMID:11081626]"
/experiment="EXISTENCE:mutant phenotype:GO:0006915
apoptotic process [PMID:18321989]"
/experiment="EXISTENCE:mutant phenotype:GO:0007135 meiosis
II [PMID:11081626]"
/experiment="EXISTENCE:mutant phenotype:GO:0031536
positive regulation of exit from mitosis
[PMID:14551257|PMID:27418100]"
/experiment="EXISTENCE:mutant phenotype:GO:0032888
regulation of mitotic spindle elongation [PMID:18430955]"
/experiment="EXISTENCE:mutant phenotype:GO:1904750
negative regulation of protein localization to nucleolus
[PMID:14551257]"
/note="Separase/separin, a caspase-like cysteine protease;
cleaves Mcd1p/Scc1p, a mitotic cohesin complex subunit,
resulting in the dissociation of cohesin from chromatin
and sister chromatid separation; cleaves meiotic-cohesin
subunit Rec8p along chromosome arms in meiosis I and at
centromeric sites during meiosis II; inhibits PP2A-Cdc55p
to promote mitotic exit; inhibited by Pds1p (securin);
relative distribution to the nucleus increases upon DNA
replication stress"
/codon_start=1
/product="separase"
/protein_id="NP_011612.3"
/db_xref="GeneID:852990"
/db_xref="SGD:S000003330"
/translation="MMVKQEEPLNEISPNTPMTSKSYLLNDTLSKVHHSGQTRPLTSV
LSGDASSNSIGILAMHNNIIRDFTKIASNNIDLAIEDITTVDHSLNSIYSLLKSHHMW
GHINSTVKQHLMIIVKLINNNALGLASSEIIFLFNETNLFQAHSLKNILLADFSTWND
YYLSNLKILALQIILKRKLVDEYLPHILELFSHDKRYLLKDPNLKAHALTKIVLSFFS
VTTSCKVLFGLKFLQYIKQFKLPFKKFISNITVECFSKNLLHKNYLEMGPNKIYLNSF
YLSYSMLYDGLDKIMLLDILSYEETTEVQRAIKSKKEFNEYCNMSENRLLWSCISVDD
LNVILENATNFLQNKGKHISATLKCLVCLWSTIRLEGLPKNKDILRQFDCTVIYINSN
IKSINDESAAALLSELLGVLSEICIDYKEPKRLSNIISVLFNASVLFKSHSFLLKTAN
LEISNVLISNDSKTSHRTILKFEKFISSAQSAQKKIEIFSCLFNVYCMLRNDTLSFVF
DFCQNAFIHCFTRLKITKFIEFSNSSEIMLSVLYGNSSIENIPSENWSQLSRMIFCSL
RGIFDLDPLELNNTFDKLHLLNKYELLIRIVYLLNLDMSKHLTTNLSKITKLYINKWL
QKSDEKAERISSFEMDFVKMLLCYLNFNNFDKLSIELSLCIKSKEKYYSSIVPYADNY
LLEAYLSLYMIDDALMMKNQLQKTMNLSTAKIEQALLHASSLINVHLWDSDLTAFQIY
FGKTLPAMKPELFDINNDHNLPMSLYIKVILLNIKIFNESAKLNIKAGNVISAVIDCR
KAQNLALSLLKKKNKLSQGSRLALLKSLSFSFFQLIKIHIRIGSARDCEFYSKELSRI
ISDLEEPIIVYRCLHFLHRYYMITEQTCLQNITLGKANKAFDYLDAEADITSLTMFLY
DNKEFVKLEQSLVLYFGDQLEKTFLPNLWKLHLGKDIDDSICLSEYMPKNVINRVHNM
WQKVMSQLEEDPFFKGMFESTLGIPSSLPVIPSTMPNNILKTPSKHSTGLKLCDSPRS
SSMTPRGKNIRQKFDRIAAISKLKQMKELLESLKLDTLDNHELSKISSLSSLTLTILS
NITSIHNAESSLITNFSLTDLPRHMPLLFDKVLNNIDNKNYREFRVSSLIAPNNISTI
TESIRVSAAQKDLMESNLNINVITIDFCPITGNLLLSKLEPRRKRRTHLRLPLIRSNS
RDLDEVHLSFPEATKKLLSIINESNQTTSVEVTNKIKTREERKSWWTTRYDLDKRMQQ
LLNNIENSWFNGVQGFFSPEVVDNSLFEKFKDKFYEILHQNLPSRKLYGNPAMFIKVE
DWVIELFLKLNPQEIDFLSKMEDLIYFVLDILLFHGEENAYDEIDFSMLHVQLEEQIK
KYRATMTTNSIFHTFLVVSSSCHLFPWECLSFLKDLSITRVPSYVCLNKLLSRFHYQL
PLQVTIEDNISMILNPNGDLSRTESKFKGMFQKIIDAKPSSQLVMNEKPEEETLLKML
QNSNLFVYIGHGGGEQYVRSKEIKKCTKIAPSFLLGCSSAAMKYYGKLEPTGTIYTYL
LGGCPMVLGNLWDVTDKDIDKFSEELFEKMGFRCNTDDLNGNSLSVSYAVSKSRGVCH
LRYLNGAAPVIYGLPIKFVS"
gene <687899..>689965
/gene="TEL2"
/locus_tag="YGR099W"
/db_xref="GeneID:852991"
mRNA <687899..>689965
/gene="TEL2"
/locus_tag="YGR099W"
/product="Tel2p"
/transcript_id="NM_001181228.3"
/db_xref="GeneID:852991"
CDS 687899..689965
/gene="TEL2"
/locus_tag="YGR099W"
/experiment="EXISTENCE:direct assay:GO:0000781 chromosome,
telomeric region [PMID:9490802]"
/experiment="EXISTENCE:direct assay:GO:0042162 telomeric
DNA binding [PMID:10525964]"
/experiment="EXISTENCE:direct assay:GO:0110078 TTT Hsp90
cochaperone complex [PMID:19040720]"
/experiment="EXISTENCE:mutant phenotype:GO:0000723
telomere maintenance [PMID:3513174]"
/experiment="EXISTENCE:mutant phenotype:GO:0007004
telomere maintenance via telomerase [PMID:8649421]"
/experiment="EXISTENCE:mutant phenotype:GO:0034502 protein
localization to chromosome [PMID:18334620]"
/note="Subunit of the TTT complex and the telomere cap
complex; subunit of the TTT Hsp90 cochaperone complex that
is involved in chromatin remodeling and telomeric
chromatin regulation, and involved in chromatin remodeling
and the telomere cap complex; DNA-binding protein specific
to single-stranded yeast telomeric DNA repeats; required
for telomere length regulation and telomere position
effect; involved in the stability or biogenesis of PIKKs
such as TORC1"
/codon_start=1
/product="Tel2p"
/protein_id="NP_011613.3"
/db_xref="GeneID:852991"
/db_xref="SGD:S000003331"
/translation="MVLETLKQGLDSSQIHEALIQLDSYPREPVDLDASMVLIKFVIP
VYPSLPERSKVILRRLASKSFTFLCQIVTFSRTISGRDGLQEIRIYQEILEDIISFEP
GCLTFYLKASTTSKADRDSIKALFFGSKLFNVLANRIDMAKYLGYLRLQWKFLLESNE
TDPPGFLGEWLVSSFLLNPVLAADMLLGELFLLKESYFFSFQKIISASSLIDQKRLIA
KFLLPYIQVIVTLENLNDVRKILRRFDLDKIISLSVLFEIQSLPLKEVIVRLMSNHSS
TKFVSALVSKFADFTDEEVDTKTCELLVLFAVHNLNHSQREEIAHDERFLNGVTKHLG
SNEREARERAMFIAKLLSGGHLKYESDFKINIPNVKFESNSDDKIIDFQSLKNPSICN
TQTDVGKDKITEVSGHVQSLTLDCSDSDDEDENDEREIVKRIVFLKDLMKEYEKTGES
RKAPLIPLLKQTVKLIRQKADFQLEVGYYAQGILSSIVCLNNEFDEPLFEQWRINALT
SILVVLPEKVNGAINILFNSELSLQQRMSLLSALGLSARELRGLDDPTIVKPKFDFPT
NRLPWDDQSHHNSRLVEVQESTSMIKKTKTVWKSRKLGKDREKGTQNRFRKYAGLFFY
PLAHGWLNGIDVGTYNQLFKSHYLTTLRIIYSCANPVHDFESMTELMNHIISSAIEEG
ISLNKG"
gene <690245..>693097
/gene="MDR1"
/locus_tag="YGR100W"
/gene_synonym="GYP2; MIC1"
/db_xref="GeneID:852992"
mRNA <690245..>693097
/gene="MDR1"
/locus_tag="YGR100W"
/gene_synonym="GYP2; MIC1"
/product="GTPase-activating protein MDR1"
/transcript_id="NM_001181229.1"
/db_xref="GeneID:852992"
CDS 690245..693097
/gene="MDR1"
/locus_tag="YGR100W"
/gene_synonym="GYP2; MIC1"
/experiment="EXISTENCE:direct assay:GO:0005096 GTPase
activator activity [PMID:10559187]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:12807768]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:35134079]"
/experiment="EXISTENCE:genetic interaction:GO:0090630
activation of GTPase activity [PMID:15574876]"
/note="Cytoplasmic GTPase-activating protein; activates
Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p;
involved in recycling of internalized proteins and
regulation of Golgi secretory function"
/codon_start=1
/product="GTPase-activating protein MDR1"
/protein_id="NP_011614.1"
/db_xref="GeneID:852992"
/db_xref="SGD:S000003332"
/translation="MSFFDSLRQKAPFLDKLADSFTPTLTRDEKFRLKYKLPANENIL
EDTNAEVSFATSIKDGKGHSDRVNNKGRKTAYVYSGRLFLTPHFLVFRDAFDHSSCVL
ILNISTIKRVERSPSESYEFALLVTLYTGAKVLIQFIGIRYRSEQFCDKLKLNLKENI
PNAKTLPAFLETSYSEFLIAKNILGKKDITVPRAGLGQHFKYPGNPTMVKEKAKLRLW
FDYFRENGRNLAVVQTPMFRKLIRIGVPNRMRGEIWELCSGAMYMRYANSGEYERILN
ENAGKTSQAIDEIEKDLKRSLPEYSAYQTEEGIQRLRNVLTAYSWKNPDVGYCQAMNI
VVAGFLIFMSEEQAFWCLCNLCDIYVPGYYSKTMYGTLLDQRVFESFVEDRMPVLWEY
ILQHDIQLSVVSLPWFLSLFFTSMPLEYAVRIMDIFFMNGSITLFQVALAVLKINADD
ILQADDDGMFIAIIKHYFQTLGQSAHPDSSDIKYRQITKFQELLVTAFKEFSVISEEM
AMHARHKYEKGIFQNIETFMKRTQLRHMPKTFNLSSDDLSNIYDMFYQSIETYKISMG
TGSSNMGFEVFIQFLSKFCDSCRPCEKDKDPAFRKQKRNFLQRLFDNWDSAHIGELTL
NDVVTGLDKLVTVDLLQAINYFFSLYDTDGDGELHREEVLQLSEGLLLLTEPWKSGRY
VDLLTKKRIEDDIAENIIKESGGEIATMNQIELPTGVTIDEEKYKVEQAERYLKAASN
FLQRSFEYAKAVDLAEEVNLIDLSDDEGEEKRTVKQKQLESIKANAALDPTHPKVIDL
PTFRMIILADETYELFFSNTLRSSVHVDEHVNIDNKNKVLRSMFDGILADGKRVAEQV
RRRVDSVATRSSIASVESTPTAAASSITTKEEKYDDLDDFTSEHQPENEELLQSSWFE
IDDANETSTKAIQERSFEPLSANSSEEKSNLIEFEA"
gene <693363..>694403
/gene="PCP1"
/locus_tag="YGR101W"
/gene_synonym="MDM37; RBD1; UGO2"
/db_xref="GeneID:852993"
mRNA <693363..>694403
/gene="PCP1"
/locus_tag="YGR101W"
/gene_synonym="MDM37; RBD1; UGO2"
/product="rhomboid protease PCP1"
/transcript_id="NM_001181230.1"
/db_xref="GeneID:852993"
CDS 693363..694403
/gene="PCP1"
/locus_tag="YGR101W"
/gene_synonym="MDM37; RBD1; UGO2"
/EC_number="3.4.21.105"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:12774122]"
/experiment="EXISTENCE:mutant phenotype:GO:0004252
serine-type endopeptidase activity
[PMID:20558178|PMID:12774122]"
/experiment="EXISTENCE:mutant phenotype:GO:0006465 signal
peptide processing [PMID:12417197]"
/experiment="EXISTENCE:mutant phenotype:GO:0010821
regulation of mitochondrion organization
[PMID:12707284|PMID:11907266]"
/note="Mitochondrial serine protease; required for the
processing of various mitochondrial proteins and
maintenance of mitochondrial DNA and morphology; belongs
to the rhomboid-GlpG superfamily of intramembrane
peptidases"
/codon_start=1
/product="rhomboid protease PCP1"
/protein_id="NP_011615.1"
/db_xref="GeneID:852993"
/db_xref="SGD:S000003333"
/translation="MSGVSSVMLGLRPATRIFFRSNISVSPSRTFVSYIGRSQSTSIL
KNAPNLEDNVTNLQKIIPKRFFSQTSILKSRWKPIFNEETTNRYVRLNRFQQYQQQRS
GGNPLGSMTILGLSLMAGIYFGSPYLFEHVPPFTYFKTHPKNLVYALLGINVAVFGLW
QLPKCWRFLQKYMLLQKDYVTSKISIIGSAFSHQEFWHLGMNMLALWSFGTSLATMLG
ASNFFSLYMNSAIAGSLFSLWYPKLARLAIVGPSLGASGALFGVLGCFSYLFPHAKIL
LFVFPVPGGAWVAFLASVAWNAAGCALRWGSFDYAAHLGGSMMGVLYGWYISKAVEKQ
RQRRLQAAGRWF"
gene complement(<694584..>695135)
/gene="GTF1"
/locus_tag="YGR102C"
/db_xref="GeneID:852994"
mRNA complement(<694584..>695135)
/gene="GTF1"
/locus_tag="YGR102C"
/product="glutamyl-tRNA(Gln) amidotransferase subunit F"
/transcript_id="NM_001181231.3"
/db_xref="GeneID:852994"
CDS complement(694584..695135)
/gene="GTF1"
/locus_tag="YGR102C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:16823961|PMID:24769239|PMID:19417106|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0030956
glutamyl-tRNA(Gln) amidotransferase complex
[PMID:19417106]"
/experiment="EXISTENCE:direct assay:GO:0050567
glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity
[PMID:19417106]"
/experiment="EXISTENCE:direct assay:GO:0070681
glutaminyl-tRNAGln biosynthesis via transamidation
[PMID:19417106]"
/note="Subunit of the trimeric GatFAB
AmidoTransferase(AdT) complex; involved in the formation
of Q-tRNAQ; transposon insertion mutant is salt sensitive
and null mutant has growth defects; non-tagged protein is
detected in purified mitochondria"
/codon_start=1
/product="glutamyl-tRNA(Gln) amidotransferase subunit F"
/protein_id="NP_011616.3"
/db_xref="GeneID:852994"
/db_xref="SGD:S000003334"
/translation="MYKTWRLCRTHTVGGLCHDGSHRFVSTGGAKIGKKFENMNQIRD
YLSRPVWSVHEYLGINTKEEKLEPPSAEAVKKLLRLSGLPLEGADIKEIQMRLAKQLS
FINKLHNIPVEGEKHTKEYDARLVQRNTKQLNYTKLLEGISHQKQDAELGEVSGSWKA
TGLAAESKNAYFVVKEGLLKNRK"
gene <695417..>697234
/gene="NOP7"
/locus_tag="YGR103W"
/gene_synonym="YPH1"
/db_xref="GeneID:852995"
mRNA <695417..>697234
/gene="NOP7"
/locus_tag="YGR103W"
/gene_synonym="YPH1"
/product="mRNA-binding ribosome synthesis protein NOP7"
/transcript_id="NM_001181232.1"
/db_xref="GeneID:852995"
CDS 695417..697234
/gene="NOP7"
/locus_tag="YGR103W"
/gene_synonym="YPH1"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10684247]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:10684247]"
/experiment="EXISTENCE:direct assay:GO:0019843 rRNA
binding [PMID:27643814]"
/experiment="EXISTENCE:direct assay:GO:0030687
preribosome, large subunit precursor [PMID:11583614]"
/experiment="EXISTENCE:direct assay:GO:0042273 ribosomal
large subunit biogenesis [PMID:27643814]"
/experiment="EXISTENCE:direct assay:GO:0070545 PeBoW
complex [PMID:16287855]"
/experiment="EXISTENCE:mutant phenotype:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12837249]"
/experiment="EXISTENCE:mutant phenotype:GO:0019843 rRNA
binding [PMID:12837249]"
/experiment="EXISTENCE:physical interaction:GO:0070545
PeBoW complex [PMID:16287855]"
/note="Component of several different pre-ribosomal
particles; forms a complex with Ytm1p and Erb1p that is
required for maturation of the large ribosomal subunit;
required for exit from G<sub>0</sub> and the initiation of
cell proliferation"
/codon_start=1
/product="mRNA-binding ribosome synthesis protein NOP7"
/protein_id="NP_011617.1"
/db_xref="GeneID:852995"
/db_xref="SGD:S000003335"
/translation="MRIKKKNTRGNARNFITRSQAVRKLQVSLADFRRLCIFKGIYPR
EPRNKKKANKGSTAPTTFYYAKDIQYLMHEPVLAKFREHKTFARKLTRALGRGEVSSA
KRLEENRDSYTLDHIIKERYPSFPDAIRDIDDALNMLFLFSNLPSTNQVSSKIINDAQ
KICNQWLAYVAKERLVRKVFVSIKGVYYQANIKGEEVRWLVPFKFPENIPSDVDFRIM
LTFLEFYSTLLHFVLYKLYTDSGLIYPPKLDLKKDKIISGLSSYILESRQEDSLLKLD
PTEIEEDVKVESLDASTLKSALNADEANTDETEKEEEQEKKQEKEQEKEQNEETELDT
FEDNNKNKGDILIQPSKYDSPVASLFSAFVFYVSREVPIDILEFLILSCGGNVISEAA
MDQIENKKDIDMSKVTHQIVDRPVLKNKVAGRTYIQPQWIFDCINKGELVPANKYLPG
EALPPHLSPWGDAIGYDPTAPVEEGEEEESESESESEDQVEEEDQEVVAGEEDDDDDE
ELQAQKELELEAQGIKYSETSEADKDVNKSKNKKRKVDEEEEEKKLKMIMMSNKQKKL
YKKMKYSNAKKEEQAENLKKKKKQIAKQKAKLNKLDSKK"
gene complement(<697445..>698368)
/gene="SRB5"
/locus_tag="YGR104C"
/gene_synonym="MED18"
/db_xref="GeneID:852996"
mRNA complement(<697445..>698368)
/gene="SRB5"
/locus_tag="YGR104C"
/gene_synonym="MED18"
/product="Srb5p"
/transcript_id="NM_001181233.3"
/db_xref="GeneID:852996"
CDS complement(697445..698368)
/gene="SRB5"
/locus_tag="YGR104C"
/gene_synonym="MED18"
/experiment="EXISTENCE:direct assay:GO:0000979 RNA
polymerase II core promoter sequence-specific DNA binding
[PMID:23447536]"
/experiment="EXISTENCE:direct assay:GO:0034605 cellular
response to heat [PMID:23447536]"
/experiment="EXISTENCE:direct assay:GO:0045944 positive
regulation of transcription by RNA polymerase II
[PMID:8187178]"
/experiment="EXISTENCE:direct assay:GO:0051123 RNA
polymerase II preinitiation complex assembly
[PMID:8324825]"
/experiment="EXISTENCE:direct assay:GO:0070847 core
mediator complex [PMID:9891034]"
/experiment="EXISTENCE:mutant phenotype:GO:0001113
transcription open complex formation at RNA polymerase II
promoter [PMID:20048049]"
/experiment="EXISTENCE:mutant phenotype:GO:0003713
transcription coactivator activity [PMID:15831453]"
/experiment="EXISTENCE:mutant phenotype:GO:0006369
termination of RNA polymerase II transcription
[PMID:23476016]"
/note="Subunit of the RNA polymerase II mediator complex;
associates with core polymerase subunits to form the RNA
polymerase II holoenzyme; essential for transcriptional
regulation; required for proper termination of
transcription for some genes; involved in telomere
maintenance"
/codon_start=1
/product="Srb5p"
/protein_id="NP_011618.3"
/db_xref="GeneID:852996"
/db_xref="SGD:S000003336"
/translation="MVQQLSLFGSIGDDGYDLLISTLTTISGNPPLLYNSLCTVWKPN
PSYDVENVNSRNQLVEPNRIKLSKEVPFSYLIDETMMDKPLNFRILKSFTNDKIPLNY
AMTRNILHNTVPQVTNFNSTNEDQNNSKHTEDTVNESRNSDDIIDVDMDASPAPSNES
CSPWSLQISDIPAAGNNRSVSMQTIAETIILSSAGKNSSVSSLMNGLGYVFEFQYLTI
GVKFFMKHGLILELQKIWQIEEAGNSQITSGGFLLKAYINVSRGTDIDRINYTETALM
NLKKELQGYIELSVPDRQSMDSRVAHGNILI"
gene <698599..>698832
/gene="VMA21"
/locus_tag="YGR105W"
/db_xref="GeneID:852997"
mRNA <698599..>698832
/gene="VMA21"
/locus_tag="YGR105W"
/product="Vma21p"
/transcript_id="NM_001181234.3"
/db_xref="GeneID:852997"
CDS 698599..698832
/gene="VMA21"
/locus_tag="YGR105W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:9660861]"
/experiment="EXISTENCE:mutant phenotype:GO:0070072
vacuolar proton-transporting V-type ATPase complex
assembly [PMID:9660861]"
/note="Integral membrane protein required for V-ATPase
function; not actual component of vacuolar H+-ATPase
(V-ATPase) complex; diverged ortholog of human VMA21/XMEA
(X-linked Myopathy with Excessive Autophagy); functions in
assembly of V-ATPase; localizes to endoplasmic reticulum
(ER)"
/codon_start=1
/product="Vma21p"
/protein_id="NP_011619.3"
/db_xref="GeneID:852997"
/db_xref="SGD:S000003337"
/translation="MAVDVPRAVINKLMLFTAAMVVLPVLTFFIIQQFTPNTLISGGL
AAAMANVVLIVYIVVAFREDTEDHKVDGNKKED"
gene complement(<698989..>699786)
/gene="VOA1"
/locus_tag="YGR106C"
/db_xref="GeneID:852998"
mRNA complement(<698989..>699786)
/gene="VOA1"
/locus_tag="YGR106C"
/product="Voa1p"
/transcript_id="NM_001181235.1"
/db_xref="GeneID:852998"
CDS complement(698989..699786)
/gene="VOA1"
/locus_tag="YGR106C"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:18799613]"
/experiment="EXISTENCE:mutant phenotype:GO:0005789
endoplasmic reticulum membrane [PMID:18799613]"
/experiment="EXISTENCE:mutant phenotype:GO:0065003
protein-containing complex assembly [PMID:18799613]"
/note="ER protein that functions in assembly of the V0
sector of V-ATPase; functions with other assembly factors;
null mutation enhances the vacuolar ATPase (V-ATPase)
deficiency of a vma21 mutant impaired in endoplasmic
reticulum (ER) retrieval"
/codon_start=1
/product="Voa1p"
/protein_id="NP_011620.1"
/db_xref="GeneID:852998"
/db_xref="SGD:S000003338"
/translation="MVFGQLYALFIFTLSCCISKTVQADSSKESSSFISFDKESNWDT
ISTISSTADVISSVDSAIAVFEFDNFSLLDNLMIDEEYPFFNRFFANDVSLTVHDDSP
LNISQSLSPIMEQFTVDELPESASDLLYEYSLDDKSIVLFKFTSDAYDLKKLDEFIDS
CLSFLEDKSGDNLTVVINSLGWAFEDEDGDDEYATEETLSHHDNNKGKEGDDDILSSI
WTEGLLMCLIVSALLLFILIVALSWISNLDITYGALEKSTNPIKKNN"
gene 700675..700756
/locus_tag="YNCG0028W"
/db_xref="GeneID:852999"
tRNA 700675..700756
/locus_tag="YNCG0028W"
/product="tRNA-Leu"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE
analysis; not essential for viability even though this is
the only tRNA that decodes GAG codons"
/db_xref="GeneID:852999"
/db_xref="SGD:S000006646"
gene complement(700953..701048)
/locus_tag="YNCG0029C"
/db_xref="GeneID:853000"
tRNA complement(join(700953..700988,701012..701048))
/locus_tag="YNCG0029C"
/product="tRNA-Lys"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE
analysis; thiolation of uridine at wobble position (34)
requires Ncs6p"
/db_xref="GeneID:853000"
/db_xref="SGD:S000006631"
repeat_region 701104..701350
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006968"
gene <703636..>705051
/gene="CLB1"
/locus_tag="YGR108W"
/gene_synonym="SCB1"
/db_xref="GeneID:853002"
mRNA <703636..>705051
/gene="CLB1"
/locus_tag="YGR108W"
/gene_synonym="SCB1"
/product="B-type cyclin CLB1"
/transcript_id="NM_001181237.1"
/db_xref="GeneID:853002"
CDS 703636..705051
/gene="CLB1"
/locus_tag="YGR108W"
/gene_synonym="SCB1"
/experiment="EXISTENCE:direct assay:GO:0000307
cyclin-dependent protein kinase holoenzyme complex
[PMID:1849458]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12972503|PMID:24223874|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:12972503]"
/experiment="EXISTENCE:direct assay:GO:0060631 regulation
of meiosis I [PMID:18423199]"
/experiment="EXISTENCE:genetic interaction:GO:0010696
positive regulation of mitotic spindle pole body
separation [PMID:1387566]"
/experiment="EXISTENCE:genetic interaction:GO:0060631
regulation of meiosis I [PMID:24223874]"
/experiment="EXISTENCE:mutant phenotype:GO:0000086 G2/M
transition of mitotic cell cycle [PMID:1427070]"
/experiment="EXISTENCE:mutant phenotype:GO:0000307
cyclin-dependent protein kinase holoenzyme complex
[PMID:1849458]"
/experiment="EXISTENCE:mutant phenotype:GO:0001932
regulation of protein phosphorylation [PMID:1849458]"
/experiment="EXISTENCE:mutant phenotype:GO:0007052 mitotic
spindle organization [PMID:1427070]"
/experiment="EXISTENCE:mutant phenotype:GO:0008315 G2/MI
transition of meiotic cell cycle [PMID:8455600]"
/experiment="EXISTENCE:mutant phenotype:GO:0016538
cyclin-dependent protein serine/threonine kinase regulator
activity [PMID:1849458]"
/experiment="EXISTENCE:mutant phenotype:GO:0060631
regulation of meiosis I [PMID:24223874]"
/note="B-type cyclin involved in cell cycle progression;
activates Cdc28p to promote the transition from G2 to M
phase; accumulates during G2 and M, then targeted via a
destruction box motif for ubiquitin-mediated degradation
by the proteasome; CLB1 has a paralog, CLB2, that arose
from the whole genome duplication"
/codon_start=1
/product="B-type cyclin CLB1"
/protein_id="NP_011622.1"
/db_xref="GeneID:853002"
/db_xref="SGD:S000003340"
/translation="MSRSLLVENSRTINSNEEKGVNESQYILQKRNVPRTILGNVTNN
ANILQEISMNRKIGMKNFSKLNNFFPLKDDVSRADDFTSSFNDSRQGVKQEVLNNKEN
IPEYGYSEQEKQQCSNDDSFHTNSTALSCNRLIYSENKSISTQMEWQKKIMREDSKKK
RPISTLVEQDDQKKFKLHELTTEEEVLEEYEWDDLDEEDCDDPLMVSEEVNDIFDYLH
HLEIITLPNKANLYKHKNIKQNRDILVNWIIKIHNKFGLLPETLYLAINIMDRFLCEE
VVQLNRLQLVGTSCLFIASKYEEIYSPSIKHFAYETDGACSVEDIKEGERFILEKLDF
QISFANPMNFLRRISKADDYDIQSRTLAKFLMEISIVDFKFIGILPSLCASAAMFLSR
KMLGKGTWDGNLIHYSGGYTKAKLYPVCQLLMDYLVGSTIHDEFLKKYQSRRFLKASI
ISIEWALKVRKNGYDIMTLHE"
gene complement(<705359..>706501)
/gene="CLB6"
/locus_tag="YGR109C"
/db_xref="GeneID:853003"
mRNA complement(<705359..>706501)
/gene="CLB6"
/locus_tag="YGR109C"
/product="B-type cyclin CLB6"
/transcript_id="NM_001181238.3"
/db_xref="GeneID:853003"
CDS complement(705359..706501)
/gene="CLB6"
/locus_tag="YGR109C"
/experiment="EXISTENCE:direct assay:GO:0001932 regulation
of protein phosphorylation [PMID:14993267]"
/experiment="EXISTENCE:direct assay:GO:0016538
cyclin-dependent protein serine/threonine kinase regulator
activity [PMID:14993267]"
/experiment="EXISTENCE:genetic interaction:GO:0000082 G1/S
transition of mitotic cell cycle
[PMID:8319908|PMID:8253070]"
/experiment="EXISTENCE:genetic interaction:GO:0006279
premeiotic DNA replication [PMID:9732268]"
/experiment="EXISTENCE:genetic interaction:GO:0045740
positive regulation of DNA replication [PMID:8319908]"
/experiment="EXISTENCE:mutant phenotype:GO:0000082 G1/S
transition of mitotic cell cycle [PMID:8253070]"
/experiment="EXISTENCE:mutant phenotype:GO:0001932
regulation of protein phosphorylation [PMID:14993267]"
/experiment="EXISTENCE:mutant phenotype:GO:0006279
premeiotic DNA replication [PMID:9732268]"
/experiment="EXISTENCE:mutant phenotype:GO:0016538
cyclin-dependent protein serine/threonine kinase regulator
activity [PMID:14993267]"
/experiment="EXISTENCE:mutant phenotype:GO:0032880
regulation of protein localization [PMID:29263158]"
/note="B-type cyclin involved in DNA replication during S
phase; activates Cdc28p to promote initiation of DNA
synthesis; functions in formation of mitotic spindles
along with Clb3p and Clb4p; most abundant during late G1;
CLB6 has a paralog, CLB5, that arose from the whole genome
duplication"
/codon_start=1
/product="B-type cyclin CLB6"
/protein_id="NP_011623.3"
/db_xref="GeneID:853003"
/db_xref="SGD:S000003341"
/translation="MNCIPSPISERKIQINNEDCIGKENAFHTIPRESSINLTPHSTN
EKKVLSEVNSNKIDSLQLPRGKLQRDSTHLEKTRKRQLSNDSTDPIEPKTVKKIKCHQ
WKNLDSIEMDDPFMVAEYTDSIFSHLYEKEIQMLPTHNYLMDTQSPYHLKSSMRALLI
DWLVEVHEKFHCLPETLFLAINLLDRFLSQNVVKLNKLQLLCITCLFIACKFEEVKLP
KITNFAYVTDGAATVEGIRKAELFVLSSLGYNISLPNPLNFIRRISKADNYCIETRNM
AKFIMEYSICCNKFIHLKPSYLAAMSMYIARKIKNENSKWDETFIHYSGGIDIESDPA
FKDFISELVEDIAVPDTNLDSLRLKYKKPKHGMVYFKVFDWCKQKR"
gene complement(707108..707179)
/locus_tag="YNCG0030C"
/db_xref="GeneID:853004"
tRNA complement(707108..707179)
/locus_tag="YNCG0030C"
/product="tRNA-Cys"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Cysteine tRNA (tRNA-Cys), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:853004"
/db_xref="SGD:S000006527"
mobile_element 707195..712545
/note="YGRWTy3-1; Ty3 element, LTR retrotransposon of the
Gypsy (Metaviridae) group; contains co-transcribed genes
TYA Gag and TYB Pol, encoding proteins involved in
structure and function of virus-like particles, flanked by
two direct repeats; transposition is induced upon exposure
to mating pheromone"
/mobile_element_type="retrotransposon:YGRWTy3-1"
/db_xref="SGD:S000006984"
repeat_region 707195..707534
/note="Ty3 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006978"
gene <707610..>712254
/locus_tag="YGR109W-B"
/db_xref="GeneID:853006"
mRNA <707610..>712254
/locus_tag="YGR109W-B"
/product="gag-pol fusion protein"
/transcript_id="NM_001184381.2"
/db_xref="GeneID:853006"
CDS join(707610..708459,708461..712254)
/locus_tag="YGR109W-B"
/EC_number="2.7.7.7"
/EC_number="2.7.7.49"
/EC_number="3.1.26.4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9448009]"
/ribosomal_slippage
/note="Retrotransposon TYA Gag and TYB Pol genes;
transcribed/translated as one unit; polyprotein is
processed to make a nucleocapsid-like protein (Gag),
reverse transcriptase (RT), protease (PR), and integrase
(IN); similar to retroviral genes"
/codon_start=1
/product="gag-pol fusion protein"
/protein_id="NP_011624.1"
/db_xref="GeneID:853006"
/db_xref="SGD:S000007347"
/translation="MSFMDQIPGGGNYPKLPVECLPNFPIQPSLTFRGRNDSHKLKNF
ISEIMLNMSMISWPNDASRIVYCRRHLLNPAAQWANDFVQEQGILEITFDTFIQGLYQ
HFYKPPDINKIFNAITQLSEAKLGIERLNQRFRKIWDRMPPDFMTEKAAIMTYTRLLT
KETYNIVRMHKPETLKDAMEEAYQTTALTERFFPGFELDADGDTIIGATTHLQEEYDS
DYDSEDNLTQNGYVHTVRTRRSYNKPMSNHRNRRNNNPSREECIKNRLCFYCKKEGHR
LNECRARRRVLTDLELESKDQQTPFIKTLPIVHYIAIPEMDNTAEKTIKIQNTKVKTL
FDSGSPTSFIRRDIVELLKYEIYETPPLRFRGFVATKSAVTSEAVTIDLKINDLHITL
AAYILDNMDYQLLIGNPILRRYPKILHTVLNTRESPDSLKPKTYRSETVNNVRTYSAG
NRGNPRNIKLSFAPTILEATDPKSAGNRGDSRTKTLSLATTTPAAIDPLTTLDNPGST
QSTFAQFPIPEEASILEEDGKYSNVVSTIQSVEPNATDHSNKDTFCTLPVWLQQKYRE
IIRNDLPPRPADINNIPVKHDIEIKPGARLPRLQPYHVTEKNEQEINKIVQKLLDNKF
IVPSKSPCSSPVVLVPKKDGTFRLCVDYRTLNKATISDPFPLPRIDNLLSRIGNAQIF
TTLDLHSGYHQIPMEPKDRYKTAFVTPSGKYEYTVMPFGLVNAPSTFARYMADTFRDL
RFVNVYLDDILIFSESPEEHWKHLDTVLERLKNENLIVKKKKCKFASEETEFLGYSIG
IQKIAPLQHKCAAIRDFPTPKTVKQAQRFLGMINYYRRFIPNCSKIAQPIQLFICDKS
QWTEKQDKAIDKLKDALCNSPVLVPFNNKANYRLTTDASKDGIGAVLEEVDNKNKLVG
VVGYFSKSLESAQKNYPAGELELLGIIKALHHFRYMLHGKHFTLRTDHISLLSLQNKN
EPARRVQRWLDDLATYDFTLEYLAGPKNVVADAISRAVYTITPETSRPIDTESWKSYY
KSDPLCSAVLIHMKELTQHNVTPEDMSAFRSYQKKLELSETFRKNYSLEDEMIYYQDR
LVVPIKQQNAVMRLYHDHTLFGGHFGVTVTLAKISPIYYWPKLQHSIIQYIRTCVQCQ
LIKSHRPRLHGLLQPLPIAEGRWLDISMDFVTGLPPTSNNLNMILVVVDRFSKRAHFI
ATRKTLDATQLIDLLFRYIFSYHGFPRTITSDRDVRMTADKYQELTKRLGIKSTMSSA
NHPQTDGQSERTIQTLNRLLRAYASTNIQNWHVYLPQIEFVYNSTPTRTLGKSPFEID
LGYLPNTPAIKSDDEVNARSFTAVELAKHLKALTIQTKEQLEHAQIEMETNNNQRRKP
LLLNIGDHVLVHRDAYFKKGAYMKVQQIYVGPFRVVKKINDNAYELDLNSHKKKHRVI
NVQFLKKFVYRPDAYPKNKPISSTERIKRAHEVTALIGIDTTHKTYLCHMQDVDPTLS
VEYSEAEFCQIPERTRRSILANFRQLYETQDNPEREEDVVSQNEICQYDNTSP"
gene <707610..>708482
/locus_tag="YGR109W-A"
/db_xref="GeneID:853005"
mRNA <707610..>708482
/locus_tag="YGR109W-A"
/product="gag protein"
/transcript_id="NM_001184380.3"
/db_xref="GeneID:853005"
CDS 707610..708482
/locus_tag="YGR109W-A"
/experiment="EXISTENCE:direct assay:GO:0000943
retrotransposon nucleocapsid [PMID:15956549]"
/experiment="EXISTENCE:direct assay:GO:0003677 DNA binding
[PMID:21917850]"
/experiment="EXISTENCE:mutant phenotype:GO:0003677 DNA
binding [PMID:21917850]"
/experiment="EXISTENCE:mutant phenotype:GO:0032197
retrotransposition [PMID:17442718]"
/note="Retrotransposon TYA Gag gene co-transcribed with
TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is
a nucleocapsid protein that is the structural constituent
of virus-like particles (VLPs); similar to retroviral Gag"
/codon_start=1
/product="gag protein"
/protein_id="NP_058162.3"
/db_xref="GeneID:853005"
/db_xref="SGD:S000007346"
/translation="MSFMDQIPGGGNYPKLPVECLPNFPIQPSLTFRGRNDSHKLKNF
ISEIMLNMSMISWPNDASRIVYCRRHLLNPAAQWANDFVQEQGILEITFDTFIQGLYQ
HFYKPPDINKIFNAITQLSEAKLGIERLNQRFRKIWDRMPPDFMTEKAAIMTYTRLLT
KETYNIVRMHKPETLKDAMEEAYQTTALTERFFPGFELDADGDTIIGATTHLQEEYDS
DYDSEDNLTQNGYVHTVRTRRSYNKPMSNHRNRRNNNPSREECIKNRLCFYCKKEGHR
LNECRARKASSNRS"
repeat_region 712206..712545
/note="Ty3 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006979"
repeat_region complement(713084..713384)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006957"
gene <713709..>715046
/gene="CLD1"
/locus_tag="YGR110W"
/db_xref="GeneID:853007"
mRNA <713709..>715046
/gene="CLD1"
/locus_tag="YGR110W"
/product="carboxylic ester hydrolase"
/transcript_id="NM_001181239.3"
/db_xref="GeneID:853007"
CDS 713709..715046
/gene="CLD1"
/locus_tag="YGR110W"
/experiment="EXISTENCE:direct assay:GO:0004623 A2-type
glycerophospholipase activity [PMID:19244244]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:19244244]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:23637464]"
/experiment="EXISTENCE:direct assay:GO:0032048 cardiolipin
metabolic process [PMID:19244244]"
/experiment="EXISTENCE:genetic interaction:GO:0032048
cardiolipin metabolic process [PMID:19244244]"
/experiment="EXISTENCE:mutant phenotype:GO:0004623 A2-type
glycerophospholipase activity [PMID:19244244]"
/experiment="EXISTENCE:mutant phenotype:GO:0035965
cardiolipin acyl-chain remodeling
[PMID:19244244|PMID:23637464]"
/note="Mitochondrial cardiolipin-specific phospholipase;
functions upstream of Taz1p to generate
monolyso-cardiolipin; transcription increases upon
genotoxic stress; involved in restricting Ty1
transposition; has homology to mammalian CGI-58"
/codon_start=1
/product="carboxylic ester hydrolase"
/protein_id="NP_011625.3"
/db_xref="GeneID:853007"
/db_xref="SGD:S000003342"
/translation="MFKSTLNSIIRRPLKGFQLLRGADSSNTRPQSPRASARDVTEKQ
ILRTPSAPTAIPLREIIYRVPSLFPRPLEDSVKDFRDFIKNEDAFQTELLKTLPFYPT
PSESKTARLIRTVVDDEGNYINEFCIRPRKTSVPEADLKHLVFIHGYGAGLGFFIKNF
EDIPLLDNEWCIHAIDLPGYGFSSRPKFPFEYPRDNIHSVQDWFHERIHTWFSKRNLL
NRPEKNIVMAHSLGSYLMALYLQKYKESPSFKKLILCSPAGVSYRDFNNTASEVEKWK
PPPWWYVKLWDRNISPFTLVRNFRQLGSKITSGWSYRRFKHILNGDPEQSKRFEALHR
YAYAIFNKRGSGEYLLSFALKCGGEPRLSLEQQLFDGKKSDILKNSNCDWLWLYGDDD
WMDVNGGLRVSRFLKEKLKQKSNVIIVPHSGHHLYLDNYKFFNNILTKEMQKI"
rep_origin 715312..715377
/note="ARS728; Autonomously Replicating Sequence"
/db_xref="SGD:S000118464"
gene <715828..>717030
/locus_tag="YGR111W"
/db_xref="GeneID:853008"
mRNA <715828..>717030
/locus_tag="YGR111W"
/product="uncharacterized protein"
/transcript_id="NM_001181240.1"
/db_xref="GeneID:853008"
CDS 715828..717030
/locus_tag="YGR111W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0008361
regulation of cell size [PMID:12089449]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to both the cytoplasm and
the nucleus"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011626.1"
/db_xref="GeneID:853008"
/db_xref="SGD:S000003343"
/translation="MTASSNDDDLIFECYSDPELKRWTHLANAKAWKGILTVQQYADR
EQLLGSSEISQKNKSNEMMTKYPKSYQWLGQKYFVLKDRSLPDNGKFSQVVSSCETLN
RIGYCIHPGSNGKIEPALIVCIGGVFTFENHRGKGYAKKMIIKLNEFYDKIRDDANTV
LELKNLVINLYSEVGEYYSALGYESMHVPLHRISKLDELTERYCGEDDDHDGKYLGFD
DYRGLVGLHETQFKESLLSLHKENPEKFVFTVAPDFDIFTWFQYRDLFIMNKSGRKAQ
QNLFFGYALSDNSHIIWHHNWNGDSLIIVKIHIPEETFQRKELKLKKLLRKAIEETKL
HGLQELEFWDEEIPIKKYPQLFQLLTELENESKVFSENGSISAVRPPKGYTAEQVIWD
NNTKFCWF"
gene <717358..>718527
/gene="SHY1"
/locus_tag="YGR112W"
/db_xref="GeneID:853009"
mRNA <717358..>718527
/gene="SHY1"
/locus_tag="YGR112W"
/product="cytochrome oxidase assembly protein SHY1"
/transcript_id="NM_001181241.1"
/db_xref="GeneID:853009"
CDS 717358..718527
/gene="SHY1"
/locus_tag="YGR112W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:9162072|PMID:15306853]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:35563734]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:15306853]"
/experiment="EXISTENCE:mutant phenotype:GO:0033617
mitochondrial respiratory chain complex IV assembly
[PMID:17882259]"
/experiment="EXISTENCE:mutant phenotype:GO:0051082
unfolded protein binding [PMID:11389896]"
/note="Mitochondrial inner membrane protein required for
complex IV assembly; associates with complex IV assembly
intermediates and complex III/complex IV supercomplexes;
similar to human SURF1 involved in Leigh Syndrome; complex
IV is also known as cytochrome c oxidase; also localizes
to the peroxisome"
/codon_start=1
/product="cytochrome oxidase assembly protein SHY1"
/protein_id="NP_011627.1"
/db_xref="GeneID:853009"
/db_xref="SGD:S000003344"
/translation="MSLLGARSTYRWFSIAASIPTKNAIGKSTYLLASRNQQYRGIIT
STVDWKPIKTGKSPNDDSRRERSFGKKIVLGLMFAMPIISFYLGTWQVRRLKWKTKLI
AACETKLTYEPIPLPKSFTPDMCEDWEYRKVILTGHFLHNEEMFVGPRKKNGEKGYFL
FTPFIRDDTGEKVLIERGWISEEKVAPDSRNLHHLSLPQEEHLKVVCLVRPPKKRGSL
QWAKKDPNSRLWQVPDIYDMARSSGCTPIQFQALYDMKDHPIIEEHTRNEASQNNSTS
SLWKFWKREPTTAVNGTQAVDNNTSKPRSRQEMPTDQTIEFDERQFIKAGVPIGRKPT
IDLKNNHLQYLVTWYGLSFLSTIFLIVALRKAKRGGVVSQDQLMKEKLKHSRKYM"
gene <718893..>719924
/gene="DAM1"
/locus_tag="YGR113W"
/db_xref="GeneID:853010"
mRNA <718893..>719924
/gene="DAM1"
/locus_tag="YGR113W"
/product="Dam1p"
/transcript_id="NM_001181242.3"
/db_xref="GeneID:853010"
CDS 718893..719924
/gene="DAM1"
/locus_tag="YGR113W"
/experiment="EXISTENCE:direct assay:GO:0008017 microtubule
binding [PMID:15664196|PMID:16777964]"
/experiment="EXISTENCE:direct assay:GO:0008608 attachment
of spindle microtubules to kinetochore [PMID:17620411]"
/experiment="EXISTENCE:direct assay:GO:0031116 positive
regulation of microtubule polymerization [PMID:15664196]"
/experiment="EXISTENCE:direct assay:GO:0042729 DASH
complex [PMID:15664196|PMID:15640796]"
/experiment="EXISTENCE:direct assay:GO:0051010 microtubule
plus-end binding [PMID:17620411|PMID:20479465]"
/experiment="EXISTENCE:direct assay:GO:0051987 positive
regulation of attachment of spindle microtubules to
kinetochore [PMID:20479468|PMID:20479465]"
/experiment="EXISTENCE:direct assay:GO:0071459 protein
localization to chromosome, centromeric region
[PMID:30903360]"
/experiment="EXISTENCE:direct assay:GO:0098653 centromere
clustering [PMID:30903360]"
/note="Essential subunit of the Dam1 complex (aka DASH
complex); cooperates with Duo1p and Pse1p to connect the
DASH complex with the microtubules (MT); couples
kinetochores to the force produced by MT depolymerization,
thereby aiding in chromosome segregation; N-terminal
staple region is important for self-assembly of the Dam1
complex higher order ring structure that forms around
microtubules; Ipl1p target for regulating kinetochore-MT
attachments"
/codon_start=1
/product="Dam1p"
/protein_id="NP_011628.4"
/db_xref="GeneID:853010"
/db_xref="SGD:S000003345"
/translation="MSEDKAKLGTTRSATEYRLSIGSAPTSRRSSMGESSSLMKFADQ
EGLTSSVGEYNENTIQQLLLPKIRELSDSIITLDSNFTRLNFIHESLADLNESLGSLL
YGIMSNSWCVEFSQAPHDIQDDLIAIKQLKSLEDEKNNLVMELSNMERGIKRKKDEQG
ENDLAKASQNKQFNQPLFPSSQVRKYRSYDNRDKRKPSKIGNNLQVENEEDYEDDTSS
EASFVLNPTNIGMSKSSQGHVTKTTRLNNNTNSKLRRKSILHTIRNSIASGADLPIEN
DNVVNLGDLHPNNRISLGSGAARVVNGPVTKNRNSMFSGRAERKPTESRHSVAKKTEK
KINTRPPFR"
gene <720409..>724764
/gene="SPT6"
/locus_tag="YGR116W"
/gene_synonym="CRE2; SSN20"
/db_xref="GeneID:853011"
mRNA <720409..>724764
/gene="SPT6"
/locus_tag="YGR116W"
/gene_synonym="CRE2; SSN20"
/product="chromatin-remodeling histone chaperone SPT6"
/transcript_id="NM_001181245.1"
/db_xref="GeneID:853011"
CDS 720409..724764
/gene="SPT6"
/locus_tag="YGR116W"
/gene_synonym="CRE2; SSN20"
/experiment="EXISTENCE:direct assay:GO:0000791 euchromatin
[PMID:15531585]"
/experiment="EXISTENCE:direct assay:GO:0001073
transcription antitermination factor activity, DNA binding
[PMID:15531585]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:2201908]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0006334 nucleosome
assembly [PMID:8633238]"
/experiment="EXISTENCE:direct assay:GO:0031491 nucleosome
binding [PMID:21094070]"
/experiment="EXISTENCE:direct assay:GO:0032968 positive
regulation of transcription elongation by RNA polymerase
II [PMID:22308335]"
/experiment="EXISTENCE:direct assay:GO:0042393 histone
binding [PMID:8633238]"
/experiment="EXISTENCE:genetic interaction:GO:0001073
transcription antitermination factor activity, DNA binding
[PMID:15531585]"
/experiment="EXISTENCE:genetic interaction:GO:0006338
chromatin remodeling [PMID:8633238]"
/experiment="EXISTENCE:genetic interaction:GO:0031440
regulation of mRNA 3'-end processing [PMID:15531585]"
/experiment="EXISTENCE:genetic interaction:GO:0031564
transcription antitermination [PMID:15531585]"
/experiment="EXISTENCE:mutant phenotype:GO:0000082 G1/S
transition of mitotic cell cycle [PMID:20502685]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:16455495]"
/experiment="EXISTENCE:mutant phenotype:GO:0001073
transcription antitermination factor activity, DNA binding
[PMID:15531585]"
/experiment="EXISTENCE:mutant phenotype:GO:0006338
chromatin remodeling [PMID:8633238]"
/experiment="EXISTENCE:mutant phenotype:GO:0006366
transcription by RNA polymerase II
[PMID:19620280|PMID:20502685|PMID:18086892|PMID:6392016]"
/experiment="EXISTENCE:mutant phenotype:GO:0016973
poly(A)+ mRNA export from nucleus [PMID:19034519]"
/experiment="EXISTENCE:mutant phenotype:GO:0031440
regulation of mRNA 3'-end processing [PMID:15531585]"
/experiment="EXISTENCE:mutant phenotype:GO:0031491
nucleosome binding [PMID:21094070]"
/experiment="EXISTENCE:mutant phenotype:GO:0031564
transcription antitermination [PMID:15531585]"
/experiment="EXISTENCE:mutant phenotype:GO:0033554
cellular response to stress [PMID:19620280]"
/experiment="EXISTENCE:mutant phenotype:GO:0034728
nucleosome organization [PMID:21098123]"
/note="Histone chaperone; regulates chromatin structure,
transcription, mRNA 3'-end formation and genome stability;
role in chromatin remodeling and nucleosome assembly;
acidic intrinsically disordered NTD binds histones and
controls chromatin structure; role in replication
initiation, promoting origin licensing through MCM
helicase loading; required for fidelity of transcriptional
promoter selection through maintenance of chromatin
structure; required for H3K36 trimethylation by Set2p"
/codon_start=1
/product="chromatin-remodeling histone chaperone SPT6"
/protein_id="NP_011631.1"
/db_xref="GeneID:853011"
/db_xref="SGD:S000003348"
/translation="MEETGDSKLVPRDEEEIVNDNDETKAPSEEEEGEDVFDSSEEDE
DIDEDEDEARKVQEGFIVNDDDENEDPGTSISKKRRKHKRREREEDDRLSEDDLDLLM
ENAGVERTKASSSSGKFKRLKRVGDEGNAAESESDNVAASRQDSTSKLEDFFSEDEEE
EESGLRNGRNNEYGRDEEDHENRNRTADKGGILDELDDFIEDDEFSDEDDETRQRRIQ
EKKLLREQSIKQPTQITGLSSDKIDEMYDIFGDGHDYDWALEIENEELENGNDNNEAE
EEEIDEETGAIKSTKKKISLQDIYDLEDLKKNLMTEGDMKIRKTDIPERYQELRAGIT
DYGNMSSEDQELERNWIAEKISVDKNFDANYDLTEFKEAIGNAIKFITKENLEVPFIY
AYRRNYISSREKDGFLLTEDDLWDIVSLDIEFHSLVNKKDYVQRFYAELHIDDPIVTE
YFKNQNTASIAELNSLQDIYDYLEFKYANEINEMFINHTGKTGKKHLKNSSYEKFKAS
PLYQAVSDIGISAEDVGENISSQHQIHPPVDHPSSKPVEVIESILNANSGDLQVFTSN
TKLAIDTVQKYYSLELSKNTKIREKVRSDFSKYYLADVVLTAKGKKEIQKGSLYEDIK
YAINRTPMHFRRDPDVFLKMVEAESLNLLSVKLHMSSQAQYIEHLFQIALETTNTSDI
AIEWNNFRKLAFNQAMDKIFQDISQEVKDNLTKNCQKLVAKTVRHKFMTKLDQAPFIP
NVRDPKIPKILSLTCGQGRFGADAIIAVYVNRKGDFIRDYKIVDNPFDKTNPEKFEDT
LDNIIQSCQPNAIGINGPNPKTQKFYKRLQEVLHKKQIVDSRGHTIPIIYVEDEVAIR
YQNSERAAQEFPNKPPLVKYCIALARYMHSPLLEYANLTSEEVRSLSIHPHQNLLSSE
QLSWALETAFVDIVNLVSVEVNKATDNNYYASALKYISGFGKRKAIDFLQSLQRLNEP
LLARQQLITHNILHKTIFMNSAGFLYISWNEKRQKYEDLEHDQLDSTRIHPEDYHLAT
KVAADALEYDPDTIAEKEEQGTMSEFIELLREDPDRRAKLESLNLESYAEELEKNTGL
RKLNNLNTIVLELLDGFEELRNDFHPLQGDEIFQSLTGESEKTFFKGSIIPVRVERFW
HNDIICTTNSEVECVVNAQRHAGAQLRRPANEIYEIGKTYPAKVIYIDYANITAEVSL
LDHDVKQQYVPISYSKDPSIWDLKQELEDAEEERKLMMAEARAKRTHRVINHPYYFPF
NGRQAEDYLRSKERGEFVIRQSSRGDDHLVITWKLDKDLFQHIDIQELEKENPLALGK
VLIVDNQKYNDLDQIIVEYLQNKVRLLNEMTSSEKFKSGTKKDVVKFIEDYSRVNPNK
SVYYFSLNHDNPGWFYLMFKINANSKLYTWNVKLTNTGYFLVNYNYPSVIQLCNGFKT
LLKSNSSKNRMNNYR"
gene complement(<725049..>726479)
/locus_tag="YGR117C"
/db_xref="GeneID:853014"
mRNA complement(<725049..>726479)
/locus_tag="YGR117C"
/product="uncharacterized protein"
/transcript_id="NM_001181246.3"
/db_xref="GeneID:853014"
CDS complement(725049..726479)
/locus_tag="YGR117C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to the cytoplasm"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011632.3"
/db_xref="GeneID:853014"
/db_xref="SGD:S000003349"
/translation="MADNSTSRFYTNLLIANYLKHNGLEDTLAAFIRETALPLSALEK
SDSSNSNVGEIPLEDLQSVVEDRIYYKRRSFKDRFKTLSINDDLAPIDNAKYGIQPWN
HSLKFSIDVKLNKSLPKDTLFISATFTEDSKYILLSSATGYLVIYDIEKATSKSFKIN
EKVKSIVKLYGPIGSSGYQYVCPMNGSFYLLNNDFELVNNAVWKIHARMITHIKICNV
TESSWFVITSGMDNFLRLSLLEIKNGNTFLTKLSEIKLASNCTSLNVIANGDGNGQNS
FSVFLTRAEYTHIACYSIIDAKNLVHSYNIALNNAEFSTYAFNIRDVMAVDYVHSNTK
DTIGLSPSTMLVVATSHKPYMRLILVEIPMNTGHPKAMKLDKVQTYYDKILRNFATEI
YQDDFSLPILGKLESSNGVLVGNDEGIYSVDLMTGDSRILNIPGEANSLHDRIKCMDI
SKDQMRMVAGTSTKSIYILNVIRNAQ"
gene <726974..>727730
/gene="RPS23A"
/locus_tag="YGR118W"
/db_xref="GeneID:853015"
mRNA join(<726974..727038,727358..>727730)
/gene="RPS23A"
/locus_tag="YGR118W"
/product="40S ribosomal protein uS12 RPS23A"
/transcript_id="NM_001181247.3"
/db_xref="GeneID:853015"
CDS join(726974..727038,727358..727730)
/gene="RPS23A"
/locus_tag="YGR118W"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:6814480]"
/experiment="EXISTENCE:direct assay:GO:0022627 cytosolic
small ribosomal subunit [PMID:6814480]"
/experiment="EXISTENCE:genetic interaction:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16246728]"
/experiment="EXISTENCE:genetic interaction:GO:0006450
regulation of translational fidelity
[PMID:8415737|PMID:7498767|PMID:8950190]"
/experiment="EXISTENCE:mutant phenotype:GO:0006450
regulation of translational fidelity
[PMID:8415737|PMID:8950190|PMID:7498767]"
/note="Ribosomal protein 28 (rp28) of the small (40S)
ribosomal subunit; required for translational accuracy;
homologous to mammalian ribosomal protein S23 and
bacterial S12; RPS23A has a paralog, RPS23B, that arose
from the whole genome duplication; deletion of both RPS23A
and RPS23B is lethal"
/codon_start=1
/product="40S ribosomal protein uS12 RPS23A"
/protein_id="NP_011633.3"
/db_xref="GeneID:853015"
/db_xref="SGD:S000003350"
/translation="MGKGKPRGLNSARKLRVHRRNNRWAENNYKKRLLGTAFKSSPFG
GSSHAKGIVLEKLGIESKQPNSAIRKCVRVQLIKNGKKVTAFVPNDGCLNFVDENDEV
LLAGFGRKGKAKGDIPGVRFKVVKVSGVSLLALWKEKKEKPRS"
gene complement(<728046..>729671)
/gene="NUP57"
/locus_tag="YGR119C"
/db_xref="GeneID:853016"
mRNA complement(<728046..>729671)
/gene="NUP57"
/locus_tag="YGR119C"
/product="FG-nucleoporin NUP57"
/transcript_id="NM_001181248.1"
/db_xref="GeneID:853016"
CDS complement(728046..729671)
/gene="NUP57"
/locus_tag="YGR119C"
/experiment="EXISTENCE:direct assay:GO:0005643 nuclear
pore [PMID:10684247]"
/experiment="EXISTENCE:direct assay:GO:0017056 structural
constituent of nuclear pore [PMID:19680227]"
/experiment="EXISTENCE:direct assay:GO:0044613 nuclear
pore central transport channel
[PMID:24574455|PMID:9813081|PMID:18046406]"
/experiment="EXISTENCE:genetic interaction:GO:0006607
NLS-bearing protein import into nucleus [PMID:15039779]"
/experiment="EXISTENCE:genetic interaction:GO:0017056
structural constituent of nuclear pore
[PMID:11387327|PMID:17875746]"
/experiment="EXISTENCE:genetic interaction:GO:0036228
protein localization to nuclear inner membrane
[PMID:21659568]"
/experiment="EXISTENCE:mutant phenotype:GO:0006606 protein
import into nucleus [PMID:9725905]"
/experiment="EXISTENCE:mutant phenotype:GO:0006999 nuclear
pore organization [PMID:9725905|PMID:14960378]"
/experiment="EXISTENCE:physical interaction:GO:0017056
structural constituent of nuclear pore [PMID:17418788]"
/note="FG-nucleoporin component of central core of the
nuclear pore complex; contributes directly to
nucleocytoplasmic transport and maintenance of the nuclear
pore complex (NPC) permeability barrier; found in stable
complex with Nic96p and two other FG-nucleoproteins (Nsp1p
and Nup49p)"
/codon_start=1
/product="FG-nucleoporin NUP57"
/protein_id="NP_011634.1"
/db_xref="GeneID:853016"
/db_xref="SGD:S000003351"
/translation="MFGFSGSNNGFGNKPAGSTGFSFGQNNNNTNTQPSASGFGFGGS
QPNSGTATTGGFGANQATNTFGSNQQSSTGGGLFGNKPALGSLGSSSTTASGTTATGT
GLFGQQTAQPQQSTIGGGLFGNKPTTTTGGLFGNSAQNNSTTSGGLFGNKVGSTGSLM
GGNSTQNTSNMNAGGLFGAKPQNTTATTGGLFGSKPQGSTTNGGLFGSGTQNNNTLGG
GGLFGQSQQPQTNTAPGLGNTVSTQPSFAWSKPSTGSNLQQQQQQQIQVPLQQTQAIA
QQQQLSNYPQQIQEQVLKCKESWDPNTTKTKLRAFVYNKVNETEAILYTKPGHVLQEE
WDQAMEKKPSPQTIPIQIYGFEGLNQRNQVQTENVAQARIILNHILEKSTQLQQKHEL
DTASRILKAQSRNVEIEKRILKLGTQLATLKNRGLPLGIAEEKMWSQFQTLLQRSEDP
AGLGKTNELWARLAILKERAKNISSQLDSKLMVFNDDTKNQDSMSKGTGEESNDRINK
IVEILTNQQRGITYLNEVLEKDAAIVKKYKNKT"
gene complement(<730033..>730821)
/gene="COG2"
/locus_tag="YGR120C"
/gene_synonym="SEC35"
/db_xref="GeneID:853017"
mRNA complement(<730033..>730821)
/gene="COG2"
/locus_tag="YGR120C"
/gene_synonym="SEC35"
/product="Golgi transport complex subunit COG2"
/transcript_id="NM_001181249.1"
/db_xref="GeneID:853017"
CDS complement(730033..730821)
/gene="COG2"
/locus_tag="YGR120C"
/gene_synonym="SEC35"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:10512869]"
/experiment="EXISTENCE:mutant phenotype:GO:0000301
retrograde transport, vesicle recycling within Golgi
[PMID:11703943]"
/experiment="EXISTENCE:mutant phenotype:GO:0000425
pexophagy [PMID:20065092]"
/experiment="EXISTENCE:mutant phenotype:GO:0006891
intra-Golgi vesicle-mediated transport [PMID:11703943]"
/experiment="EXISTENCE:mutant phenotype:GO:0016236
macroautophagy [PMID:20065092]"
/experiment="EXISTENCE:mutant phenotype:GO:0017119 Golgi
transport complex [PMID:11703943]"
/experiment="EXISTENCE:mutant phenotype:GO:0032258
cytoplasm to vacuole targeting by the Cvt pathway
[PMID:20065092]"
/note="Essential component of the conserved oligomeric
Golgi complex; a cytosolic tethering complex that
functions in protein trafficking to mediate fusion of
transport vesicles to Golgi compartments; the components
of the Golgi complex are Gog1p through Cog8p"
/codon_start=1
/product="Golgi transport complex subunit COG2"
/protein_id="NP_011635.2"
/db_xref="GeneID:853017"
/db_xref="SGD:S000003352"
/translation="MDFLNDDELDLDLPVTAEISKELFATEIEKYRESETNGTDVDNF
DVDRFLVQKNFHYLPLDSLIRDLSGLSQKMVQTLLEQIRSNYDDYLTFSNTYTDEENE
TLINLEKTQSDLQKFMTQLDHLIKDDISNTQEIIKDVLEYLKKLDEIYGSLRNHSQLT
EALSLGKRLSKSLHEMCGIEPLEEEICSGLIEQLYKLITASRRILESCADSNSPYIHH
LRNDYQDLLQEFQISLKILTEKCLENPSSLQNLSLTLVSIIKTA"
gene 731137..731210
/locus_tag="YNCG0031W"
/db_xref="GeneID:853018"
tRNA 731137..731210
/locus_tag="YNCG0031W"
/product="tRNA-Asn"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Asparagine tRNA (tRNA-Asn), predicted by
tRNAscan-SE analysis"
/db_xref="GeneID:853018"
/db_xref="SGD:S000006670"
gene complement(<731449..>732927)
/gene="MEP1"
/locus_tag="YGR121C"
/gene_synonym="AMT1"
/db_xref="GeneID:853019"
mRNA complement(<731449..>732927)
/gene="MEP1"
/locus_tag="YGR121C"
/gene_synonym="AMT1"
/product="ammonium permease MEP1"
/transcript_id="NM_001181250.3"
/db_xref="GeneID:853019"
CDS complement(731449..732927)
/gene="MEP1"
/locus_tag="YGR121C"
/gene_synonym="AMT1"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:26172854]"
/experiment="EXISTENCE:direct assay:GO:0008519 ammonium
channel activity [PMID:9234685]"
/experiment="EXISTENCE:mutant phenotype:GO:0008519
ammonium channel activity [PMID:9234685]"
/experiment="EXISTENCE:mutant phenotype:GO:0019740
nitrogen utilization [PMID:9234685]"
/experiment="EXISTENCE:mutant phenotype:GO:0072488
ammonium transmembrane transport [PMID:9234685]"
/note="Ammonium permease; belongs to Mep-Amt-Rh family of
well-conserved ammonium (NH4+) transporters that includes
the human Rh factors; expression is under the nitrogen
catabolite repression regulation; activity regulated by
TORC1 effectors, Npr1p and Par32p; human homolog RHCG
complements yeast null mutant; mutations in human homolog
RHCG implicated in metabolic acidosis; MEP1 has a paralog,
MEP3, that arose from the whole genome duplication"
/codon_start=1
/product="ammonium permease MEP1"
/protein_id="NP_011636.3"
/db_xref="GeneID:853019"
/db_xref="SGD:S000003353"
/translation="MESRTTGPLTTETYDGPTVAFMILGAALVFFMVPGLGFLYSGLA
RRKSALALIWVVLMATLVGILQWYFWGYSLAFSKSAPNNKFIGNLDSFGFRNVYGKKF
DEDAYPELAYATFQMMFSCVNLSIIAGATAERGRLLPHMVFLFILATIGYCPVTYWIW
SPGGWAYQWGVLDWAGGGNIEILSAVSGFVYSWFLGKRNEKLLINFRPHNVSLVTLGT
SILWFGWLLFNSASSLSPNLRSVYAFMNTCLSAITGGMTWCLLDYRSEKKWSTVGLCS
GIISGLVAATPSSGCITLYGSLIQGIVAGVVCNFATKLKYYAKVDDAMDILAEHGVAG
VIGLIFNALFGADWVIGMDGTTEHEGGWVTHNYKQMYKQIAYIAASIGYTAAVTAIIC
FVLGYIPGMRLRISEEAEEAGMDEDQIGEFAYDYVEVRRDYYLWGVDEDSQRSDVNHR
VNNAHLAAERSSSGTNSSSDGNGEMIQSEKILPIHQEDPANR"
gene <733412..>733627
/locus_tag="YGR121W-A"
/db_xref="GeneID:1466458"
mRNA <733412..>733627
/locus_tag="YGR121W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184551.1"
/db_xref="GeneID:1466458"
CDS 733412..733627
/locus_tag="YGR121W-A"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878078.1"
/db_xref="GeneID:1466458"
/db_xref="SGD:S000028550"
/translation="MSRHKYKLWMCIAKGKRGVGERNTFVPKCSYAPFCASEVLDQLL
RGDNSIVYNFYLKNGSGSVYIGSVFLI"
gene <733935..>735143
/locus_tag="YGR122W"
/db_xref="GeneID:853020"
mRNA <733935..>735143
/locus_tag="YGR122W"
/product="uncharacterized protein"
/transcript_id="NM_001181251.3"
/db_xref="GeneID:853020"
CDS 733935..735143
/locus_tag="YGR122W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:22842922]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:16024810|PMID:15879523]"
/experiment="EXISTENCE:mutant phenotype:GO:0070481
nuclear-transcribed mRNA catabolic process, non-stop decay
[PMID:17660569]"
/note="Protein that may be involved in pH regulation;
probable ortholog of A. nidulans PalC, which is involved
in pH regulation and binds to the ESCRT-III complex; null
mutant does not properly process Rim101p and has decreased
resistance to rapamycin; GFP-fusion protein is
cytoplasmic; relative distribution to cytoplasm increases
upon DNA replication stress"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011637.3"
/db_xref="GeneID:853020"
/db_xref="SGD:S000003354"
/translation="MRENAMSTKKLPYKLSGSSKITTSIPNDLIILRNNCINSLNSSS
SKADSITCIDTWLKYTEGLLTHRYEANNDAALIEEEIAIALINVAVFYQDIGIETLYR
AYESSQASNNLWTTSGTYLKRGLGLICFLGKNFQINTANDCQKMQVLNVLNQLSLEFQ
LLQQLGIVVLALSKLRSKISKDAVADLEPQELEELGKSSVFYAKLCIGSYSTASQCQG
GRIVDALFMNYLQSLTYLFLSINQYNNDECGIAIGMLQESIKKLLNIVPNSQLKELDI
LSSTDITKKRDLIKMSFKRKIHGSTLKNQRIFEKKVPFSSKAYMMPLLKSSLDDFVIP
LTILLRYRYQTTNENYSFKTVETDVSKLKELFPRGKSSDIEGTVWSFQDGHLTFADSN
NATHNCGNYF"
repeat_region 735542..735872
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006969"
repeat_region 735982..736322
/note="Ty3 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006980"
gene 736340..736411
/locus_tag="YNCG0032W"
/db_xref="GeneID:853022"
tRNA 736340..736411
/locus_tag="YNCG0032W"
/product="tRNA-Arg"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE
analysis; one of 11 nuclear tRNA genes containing the
tDNA-anticodon UCU (converted to mcm5-UCU in the mature
tRNA), decodes AGA codons into arginine, one of 19 nuclear
tRNAs for arginine"
/db_xref="GeneID:853022"
/db_xref="SGD:S000006713"
gene complement(<736662..>738203)
/gene="PPT1"
/locus_tag="YGR123C"
/db_xref="GeneID:853023"
mRNA complement(<736662..>738203)
/gene="PPT1"
/locus_tag="YGR123C"
/product="protein serine/threonine phosphatase"
/transcript_id="NM_001181252.3"
/db_xref="GeneID:853023"
CDS complement(736662..738203)
/gene="PPT1"
/locus_tag="YGR123C"
/EC_number="3.1.3.16"
/experiment="EXISTENCE:direct assay:GO:0004722 protein
serine/threonine phosphatase activity [PMID:12694636]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12694636]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:12694636]"
/experiment="EXISTENCE:direct assay:GO:0006470 protein
dephosphorylation [PMID:7925273]"
/note="Protein serine/threonine phosphatase; regulates
Hsp90 chaperone by affecting its ATPase and cochaperone
binding activities; has similarity to human phosphatase
PP5; present in both the nucleus and cytoplasm; expressed
during logarithmic growth"
/codon_start=1
/product="protein serine/threonine phosphatase"
/protein_id="NP_011639.3"
/db_xref="GeneID:853023"
/db_xref="SGD:S000003355"
/translation="MSTPTAADRAKALERKNEGNVFVKEKHFLKAIEKYTEAIDLDST
QSIYFSNRAFAHFKVDNFQSALNDCDEAIKLDPKNIKAYHRRALSCMALLEFKKARKD
LNVLLKAKPNDPAATKALLTCDRFIREERFRKAIGGAENEAKISLCQTLNLSSFDANA
DLANYEGPKLEFEQLYDDKNAFKGAKIKNMSQEFISKMVNDLFLKGKYLPKKYVAAII
SHADTLFRQEPSMVELENNSTPDVKISVCGDTHGQFYDVLNLFRKFGKVGPKHTYLFN
GDFVDRGSWSCEVALLFYCLKILHPNNFFLNRGNHESDNMNKIYGFEDECKYKYSQRI
FNMFAQSFESLPLATLINNDYLVMHGGLPSDPSATLSDFKNIDRFAQPPRDGAFMELL
WADPQEANGMGPSQRGLGHAFGPDITDRFLRNNKLRKIFRSHELRMGGVQFEQKGKLM
TVFSAPNYCDSQGNLGGVIHVVPGHGILQAGRNDDQNLIIETFEAVEHPDIKPMAYSN
GGFGL"
gene 739122..739195
/locus_tag="YNCG0033W"
/db_xref="GeneID:853024"
tRNA 739122..739195
/locus_tag="YNCG0033W"
/product="tRNA-Ile"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Isoleucine tRNA (tRNA-Ile), predicted by
tRNAscan-SE analysis"
/db_xref="GeneID:853024"
/db_xref="SGD:S000006604"
gene <739944..>741662
/gene="ASN2"
/locus_tag="YGR124W"
/db_xref="GeneID:853025"
mRNA <739944..>741662
/gene="ASN2"
/locus_tag="YGR124W"
/product="asparagine synthase (glutamine-hydrolyzing) 2"
/transcript_id="NM_001181253.1"
/db_xref="GeneID:853025"
CDS 739944..741662
/gene="ASN2"
/locus_tag="YGR124W"
/EC_number="6.3.5.4"
/experiment="EXISTENCE:direct assay:GO:0004066 asparagine
synthase (glutamine-hydrolyzing) activity [PMID:6105958]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0070981
L-asparagine biosynthetic process [PMID:8951815]"
/note="Asparagine synthetase; catalyzes the synthesis of
L-asparagine from L-aspartate in the asparagine
biosynthetic pathway; ASN2 has a paralog, ASN1, that arose
from the whole genome duplication"
/codon_start=1
/product="asparagine synthase (glutamine-hydrolyzing) 2"
/protein_id="NP_011640.1"
/db_xref="GeneID:853025"
/db_xref="SGD:S000003356"
/translation="MCGIFAAFKHEDIHNFKPKALQLSKKIRHRGPDWSGNAVMNSTI
FVHERLAIVGLDSGAQPITSADGEYMLGVNGEIYNHIQLREMCSDYKFQTFSDCEPII
PLYLEHDIDAPKYLDGMFAFCLYDSKKDRIVAARDPIGVVTLYMGRSSQSPETVYFAS
ELKCLTDVCDSIISFPPGHVYDSETDKITRYFTPDWLDEKRIPSTPVDYHAIRHSLEK
AVRKRLMAEVPYGVLLSGGLDSSLIAAIAARETEKANADANEDNNVDEKQLAGIDDQG
HLHTSGWSRLHSFAIGLPNAPDLQAARKVAKFIGSIHHEHTFTLQEGLDALDDVIYHL
ETYDVTTIRASTPMFLLSRKIKAQGVKMVLSGEGSDEIFGGYLYFAQAPSAAEFHTES
VQRVKNLHLADCLRANKSTMAWGLEARVPFLDKDFLQLCMNIDPNEKMIKPKEGRIEK
YILRKAFDTTDEPDVKPYLPEEILWRQKEQFSDGVGYSWIDGLRDTAERAISDAMFAN
PKADWGDDIPTTKEAYWYRLKFDAWFPQKTAADTVMRWIPKADWGCAEDPSGRYAKIH
EKHVSA"
gene <742325..>745435
/gene="VSB1"
/locus_tag="YGR125W"
/db_xref="GeneID:853026"
mRNA <742325..>745435
/gene="VSB1"
/locus_tag="YGR125W"
/product="Vsb1p"
/transcript_id="NM_001181254.1"
/db_xref="GeneID:853026"
CDS 742325..745435
/gene="VSB1"
/locus_tag="YGR125W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:14562095|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:33704406]"
/experiment="EXISTENCE:mutant phenotype:GO:0000329
fungal-type vacuole membrane [PMID:32776922]"
/experiment="EXISTENCE:mutant phenotype:GO:0034490 basic
amino acid transmembrane import into vacuole
[PMID:32776922]"
/experiment="EXISTENCE:mutant phenotype:GO:0034618
arginine binding [PMID:33704406]"
/note="Vacuolar membrane protein required for uptake and
storage of arginine in nitrogen-replete cells; nitrogen
starvation appears to inhibit Vsb1-dependent activity,
resulting in mobilization of stored vacuolar arginine to
the cytosol; arginine uptake appears to be driven by a
V-ATPase mediated H+ gradient; member of the APC (amino
acid-polyamine-organocation) transporter superfamily"
/codon_start=1
/product="Vsb1p"
/protein_id="NP_011641.1"
/db_xref="GeneID:853026"
/db_xref="SGD:S000003357"
/translation="MGRTIRRRRSNSSLSEAISVSLGINQDSSVNKMHRASVSAMSPP
LCRSYMSGFFTGGNSPMINNLSDSKLPISNKQHPKVIHGSENLHRQTAQLSNEFCSSS
VEENSPTIKDYMDIIGNGDRKDDQSMRTIEENIDEEYSDEYSRLLLSPASSNVDDDRN
RGLQNSSLPELEDGYAGGYQSLRPSHNLRFRPRNLWHMCTSFPSKFAHYLPAAVLGLL
LNILDALSYGMIIFPITEPVFSHLGPTGISMFYISTIISQAVYSGGWSSFPSGIGSEM
IEITPFYHTMALAIKEALAGNDDEIITTTIFCYVISSMLTGVVFYALGKLRLGKIVGF
FPRHILIGCIGGVGYFLIITGIEVTTRVAKFEYSWPFFSGLFTDYDTLAKWLLPVLLT
VVLIGTQRYFKNSLVLPSFYILTLVLFHFIVAIIPTLSLDALRQAGWIFPIANSDSKW
YDHYRLFNVHKVHWSLVLQQIPTMMALTFFGILHVPINVPALAMSLQMDKYDVDRELI
AHGYSNFFSGLLGSVQNYLVYTNSVLFIRAGADSPFAGFLLIALTICIMIIGPVIISF
IPICIVGSLIFLLGYELLVEALVDTWNKLNRFEYLTVVIIVFTMGIFDFVLGIIVGIL
IACFSFLVDSTKLQTINGEYNGNVARSTVYRDYVQTKFLDGIGEQIYVLKLQNLLFFG
TIISIEEKIERLLQISNKDATKRRIKYLILDFKNINADNIDYSAAEGFNRIKRFTETK
RIKLIISSIKERDRIYNAFNNVGLLNDVELFADLNSALEWCENEFLFQYKQLRKKAKE
RLEEGKQNNVVSAVIAATKNKKIDTIGNGLNRGSNGDTARNLMSLPTNTPRNYQILSV
AQNVFVNDEQAVKNFKKEYKDDEPVLPILLFALKQYRPDIISEVQKVREKEIKFWAQL
CPYFTRRRLASQSHLLHADNIFFLVETGMLKATYELPQGTLYEIFSNGTCFGKIIAPG
NAMPREQKLTIETETDSVLWVIDSSSLNKLKEDNLALYVEVALMVMCIKDTRFKELLG
YTLVSA"
gene <745835..>746527
/locus_tag="YGR126W"
/db_xref="GeneID:853027"
mRNA <745835..>746527
/locus_tag="YGR126W"
/product="uncharacterized protein"
/transcript_id="NM_001181255.3"
/db_xref="GeneID:853027"
CDS 745835..746527
/locus_tag="YGR126W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to both the cytoplasm and
the nucleus and is induced in response to the DNA-damaging
agent MMS"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011642.3"
/db_xref="GeneID:853027"
/db_xref="SGD:S000003358"
/translation="MPVPSVTVTTDNEYEDISSFSSIDSYKPEPFTGFKDSEAPEQPL
LKNDTIVGKGQLEDDSNVDDQHRHSDVHSHHSSSTLKRPTSNSIEKMVTHNALEGNSE
TVDSLKEDGLNLNKKALPDITAPVTNSAHDAAFPEEYRLETETGLVKLKTLESLKRED
SRVSSTKKEHINDHTDMHSTRSKVTTNSQGSSLEPNKLNMAVEKNKKRIEKYQKHKSE
KGIKGFFHRIFD"
gene <746798..>747736
/locus_tag="YGR127W"
/db_xref="GeneID:853028"
mRNA <746798..>747736
/locus_tag="YGR127W"
/product="uncharacterized protein"
/transcript_id="NM_001181256.1"
/db_xref="GeneID:853028"
CDS 746798..747736
/locus_tag="YGR127W"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:36164978]"
/note="Protein involved in heme trafficking to the
nucleus; expression is regulated by Msn2p/Msn4p,
indicating possible role in stress response; localizes to
cytosol and peroxisomes"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011643.1"
/db_xref="GeneID:853028"
/db_xref="SGD:S000003359"
/translation="MCILMATRAHPDYELILISNRDEFLARKTHATCWHNNDFILSPY
DLAKTSAEKQIFGTWSGINKEGKLATILNLKLDNEQNNTKSRSRGLLPFIFLSIHKAD
FEDWDNYKKFEGHYDGLKSTGDFNFFYGDVIKKQYKVIDSLGRTFDVLSSTCRKDLDS
YMVVSNGKFYDSSSIPGQAWEKVKVARDSLENLVLENIESDEEKIISSCFQLASKSSL
PSTISNPDVLQMVDPNVTMNTIYVPPLRRPPRDDLGASIPDGDYYGTRSQIVLLVSKD
STRVTFIERVLYSSDEDVRKYSVTSPKEEKRFKFKL"
gene complement(<747950..>750091)
/gene="UTP8"
/locus_tag="YGR128C"
/db_xref="GeneID:853029"
mRNA complement(<747950..>750091)
/gene="UTP8"
/locus_tag="YGR128C"
/product="Utp8p"
/transcript_id="NM_001181257.3"
/db_xref="GeneID:853029"
CDS complement(747950..750091)
/gene="UTP8"
/locus_tag="YGR128C"
/experiment="EXISTENCE:direct assay:GO:0000049 tRNA
binding [PMID:19370060]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:12068309]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/experiment="EXISTENCE:direct assay:GO:0032040
small-subunit processome [PMID:12068309]"
/experiment="EXISTENCE:direct assay:GO:0033553 rDNA
heterochromatin [PMID:15489292]"
/experiment="EXISTENCE:direct assay:GO:0034455 t-UTP
complex [PMID:17515605]"
/experiment="EXISTENCE:direct assay:GO:0034511 U3 snoRNA
binding [PMID:12068309]"
/experiment="EXISTENCE:mutant phenotype:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12068309]"
/experiment="EXISTENCE:mutant phenotype:GO:0006409 tRNA
export from nucleus [PMID:12794079]"
/experiment="EXISTENCE:mutant phenotype:GO:0045943
positive regulation of transcription by RNA polymerase I
[PMID:15489292]"
/note="Nucleolar protein required for export of tRNAs from
the nucleus; also copurifies with the small subunit (SSU)
processome containing the U3 snoRNA that is involved in
processing of pre-18S rRNA"
/codon_start=1
/product="Utp8p"
/protein_id="NP_011644.3"
/db_xref="GeneID:853029"
/db_xref="SGD:S000003360"
/translation="MPSLSQPFRLATLPKIASLSNFSLQADYVQVADGTFNESTNNIT
LGISGSSISQYIINPTPKLTFDYPIPSTNIITACNAEKGQANIDGNIEASTDDEANNE
KTINTQKKRNVEIWAFGLMVNKGNYTLNVITKALEDTTDTSNDHLSESDIDNKAYTGS
DEFLSQYKIKAKAKVMSIKIDTKNSLVIAILQNGLIEIFDFKLTLLHSFDISYDNLKY
AKWFTENGTEYVFVLCPLQDDKVCYKLLELTDCGSGESSPIKELSSTIIEGFSFENSK
LCYQFGKLYKLNQGKIYIYSLPHCQLQQVIEFPMVDKLSPGDDLISFQPVSVNRVLLT
VNNVIYLLDLLHCSTLSQRELTHVKTFQLLKSAVINSEKSHNSKTIAIGISTKNGPNP
TSSLEIINIDVGTNTLKDSLGKSFQVGNNDSSVILKPLFDDKDINDKRVKCNDVSGDS
SVPVLHCNEVIEKLSALQDNDITSFDDIFFKELKIKEEHYTEKDRYISDPGFLNKVLD
LIFGKFSGNDYPKTLTFLLTHPLFPLSRTRNLLSLLRDQPRLFKQAIVTCPNLPLNEL
LEELFSIRNRELLLDISFRILQDFTRDSIKQEMKKLSKLDVQNFIEFITSGGEDSSPE
CFNPSQSTQLFQLLSLVLDSIGLFSLEGALLENLTLYIDKQVEIAERNTELWNLIDTK
GFQHGFASSTFDNGTSQKRALPTYTMEYLDI"
gene <750400..>751047
/gene="SYF2"
/locus_tag="YGR129W"
/gene_synonym="NTC31"
/db_xref="GeneID:853030"
mRNA <750400..>751047
/gene="SYF2"
/locus_tag="YGR129W"
/gene_synonym="NTC31"
/product="Syf2p"
/transcript_id="NM_001181258.3"
/db_xref="GeneID:853030"
CDS 750400..751047
/gene="SYF2"
/locus_tag="YGR129W"
/gene_synonym="NTC31"
/experiment="EXISTENCE:curator inference:GO:0000384 first
spliceosomal transesterification activity
[PMID:11842115|PMID:11105756]"
/experiment="EXISTENCE:curator inference:GO:0000386 second
spliceosomal transesterification activity [PMID:11105756]"
/experiment="EXISTENCE:direct assay:GO:0000974 Prp19
complex [PMID:11842115]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0071004 U2-type
prespliceosome [PMID:11105756]"
/experiment="EXISTENCE:direct assay:GO:0071006 U2-type
catalytic step 1 spliceosome
[PMID:11105756|PMID:11842115]"
/experiment="EXISTENCE:direct assay:GO:0071007 U2-type
catalytic step 2 spliceosome [PMID:11105756]"
/experiment="EXISTENCE:direct assay:GO:0071008 U2-type
post-mRNA release spliceosomal complex [PMID:11105756]"
/experiment="EXISTENCE:mutant phenotype:GO:0000398 mRNA
splicing, via spliceosome [PMID:11105756]"
/note="Member of the NineTeen Complex (NTC); NTC contains
Prp19p and stabilizes U6 snRNA in catalytic forms of the
spliceosome containing U2, U5, and U6 snRNAs; relocalizes
to the cytosol in response to hypoxia; isy1 syf2 cells
have defective spindles activiating cell cycle arrest"
/codon_start=1
/product="Syf2p"
/protein_id="NP_011645.3"
/db_xref="GeneID:853030"
/db_xref="SGD:S000003361"
/translation="MDFYKLDEKLKELKRKRVDVSIKSRKLADREIQEVSANRKPRVY
SMEDVNDADESVGDTESPEKEKAFHYTVQEYDAWERRHPQGKTGQSQRGGISYDQLAK
LSYEKTLRNLATQTQNSSKQDSSADEEDNKNVPKKGRIGKVQKDTKTGKITIADDDKL
VNKLAVSLQSESKKRYEARKRQMQNAKTLYGVESFINDKNKQFNEKLSRESKGSE"
gene complement(<751394..>753844)
/locus_tag="YGR130C"
/db_xref="GeneID:853031"
mRNA complement(<751394..>753844)
/locus_tag="YGR130C"
/product="uncharacterized protein"
/transcript_id="NM_001181259.1"
/db_xref="GeneID:853031"
CDS complement(751394..753844)
/locus_tag="YGR130C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0032126 eisosome
[PMID:19064668|PMID:19269952]"
/experiment="EXISTENCE:mutant phenotype:GO:0042149
cellular response to glucose starvation [PMID:33443082]"
/experiment="EXISTENCE:mutant phenotype:GO:0097446 protein
localization to eisosome filament [PMID:33443082]"
/note="Component of the eisosome with unknown function;
GFP-fusion protein localizes to the cytoplasm;
specifically phosphorylated in vitro by mammalian
diphosphoinositol pentakisphosphate (IP7)"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011646.1"
/db_xref="GeneID:853031"
/db_xref="SGD:S000003362"
/translation="MLFNINRQEDDPFTQLINQSSANTQNQQAHQQESPYQFLQKVVS
NEPKGKEEWVSPFRQDALANRQNNRAYGEDAKNRKFPTVSATSAYSKQQPKDLGYKNI
PKNAKRAKDIRFPTYLTQNEERQYQLLTELELKEKHLKYLKKCQKITDLTKDEKDDTD
TTTSSSTSTSSSSSSSSSSSSSSSSDEGDVTSTTTSEATEATADTATTTTTTTSTSTT
STSTTNAVENSADEATSVEEEHEDKVSESTSIGKGTADSAQINVAEPISSENGVLEPR
TTDQSGGSKSGVVPTDEQKEEKSDVKKVNPPSGEEKKEVEAEGDAEEETEQSSAEESA
ERTSTPETSEPESEEDESPIDPSKAPKVPFQEPSRKERTGIFALWKSPTSSSTQKSKT
AAPSNPVATPENPELIVKTKEHGYLSKAVYDKINYDEKIHQAWLADLRAKEKDKYDAK
NKEYKEKLQDLQNQIDEIENSMKAMREETSEKIEVSKNRLVKKIIDVNAEHNNKKLMI
LKDTENMKNQKLQEKNEVLDKQTNVKSEIDDLNNEKTNVQKEFNDWTTNLSNLSQQLD
AQIFKINQINLKQGKVQNEIDNLEKKKEDLVTQTEENKKLHEKNVQVLESVENKEYLP
QINDIDNQISSLLNEVTIIKQENANEKTQLSAITKRLEDERRAHEEQLKLEAEERKRK
EENLLEKQRQELEEQAHQAQLDHEQQITQVKQTYNDQLTELQDKLATEEKELEAVKRE
RTRLQAEKAIEEQTRQKNADEALKQEILSRQHKQAEGIHAAENHKIPNDRSQKNTSVL
PKDDSLYEYHTEEDVMYA"
gene <754726..>755250
/gene="FHN1"
/locus_tag="YGR131W"
/db_xref="GeneID:853032"
mRNA <754726..>755250
/gene="FHN1"
/locus_tag="YGR131W"
/product="Fhn1p"
/transcript_id="NM_001181260.1"
/db_xref="GeneID:853032"
CDS 754726..755250
/gene="FHN1"
/locus_tag="YGR131W"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0072659 protein
localization to plasma membrane [PMID:20581291]"
/note="hypothetical protein; induced by ketoconazole;
promoter region contains sterol regulatory element motif,
which has been identified as a Upc2p-binding site;
overexpression complements function of Nce102p in NCE102
deletion strain; FHN1 has a paralog, NCE102, that arose
from the whole genome duplication"
/codon_start=1
/product="Fhn1p"
/protein_id="NP_011647.1"
/db_xref="GeneID:853032"
/db_xref="SGD:S000003363"
/translation="MLSAADNLVRIINAVFLIISIGLISGLIGTQTKHSSRVNFCMFA
AVYGLVTDSLYGFLANFWTSLTYPAILLVLDFLNFIFTFVAATALAVGIRCHSCKNKT
YLEQNKIIQGSSSRCHQSQAAVAFFYFSCFLFLIKVTVATMGMMQNGGFGSNTGFSRR
RARRQMGIPTISQV"
gene complement(<755589..>756452)
/gene="PHB1"
/locus_tag="YGR132C"
/db_xref="GeneID:853033"
mRNA complement(<755589..>756452)
/gene="PHB1"
/locus_tag="YGR132C"
/product="prohibitin subunit PHB1"
/transcript_id="NM_001181261.3"
/db_xref="GeneID:853033"
CDS complement(755589..756452)
/gene="PHB1"
/locus_tag="YGR132C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:24769239|PMID:14576278|PMID:9259555|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:10835343|PMID:9632789]"
/experiment="EXISTENCE:direct assay:GO:0006457 protein
folding [PMID:10835343]"
/experiment="EXISTENCE:genetic interaction:GO:0007007
inner mitochondrial membrane organization [PMID:19221197]"
/experiment="EXISTENCE:mutant phenotype:GO:0000001
mitochondrion inheritance [PMID:12882345]"
/experiment="EXISTENCE:mutant phenotype:GO:0000423
mitophagy [PMID:38964378]"
/experiment="EXISTENCE:mutant phenotype:GO:0007005
mitochondrion organization [PMID:19221197]"
/experiment="EXISTENCE:mutant phenotype:GO:0007007 inner
mitochondrial membrane organization [PMID:19221197]"
/experiment="EXISTENCE:mutant phenotype:GO:0045861
negative regulation of proteolysis [PMID:10207067]"
/note="Subunit of the prohibitin complex (Phb1p-Phb2p);
prohibitin is a 1.2 MDa ring-shaped inner mitochondrial
membrane chaperone that stabilizes newly synthesized
proteins; determinant of replicative life span; involved
in mitochondrial segregation; prohibitin deficiency
induces a mitochondrial unfolded protein response (mtUPR)"
/codon_start=1
/product="prohibitin subunit PHB1"
/protein_id="NP_011648.3"
/db_xref="GeneID:853033"
/db_xref="SGD:S000003364"
/translation="MSNSAKLIDVITKVALPIGIIASGIQYSMYDVKGGSRGVIFDRI
NGVKQQVVGEGTHFLVPWLQKAIIYDVRTKPKSIATNTGTKDLQMVSLTLRVLHRPEV
LQLPAIYQNLGLDYDERVLPSIGNEVLKSIVAQFDAAELITQREIISQKIRKELSTRA
NEFGIKLEDVSITHMTFGPEFTKAVEQKQIAQQDAERAKFLVEKAEQERQASVIRAEG
EAESAEFISKALAKVGDGLLLIRRLEASKDIAQTLANSSNVVYLPSQHSGGGNSESSG
SPNSLLLNIGR"
gene <756896..>757447
/gene="PEX4"
/locus_tag="YGR133W"
/gene_synonym="PAS2; UBC10"
/db_xref="GeneID:853034"
mRNA <756896..>757447
/gene="PEX4"
/locus_tag="YGR133W"
/gene_synonym="PAS2; UBC10"
/product="E2 ubiquitin-protein ligase peroxin 4"
/transcript_id="NM_001181262.1"
/db_xref="GeneID:853034"
CDS 756896..757447
/gene="PEX4"
/locus_tag="YGR133W"
/gene_synonym="PAS2; UBC10"
/EC_number="2.3.2.23"
/experiment="EXISTENCE:direct assay:GO:0004842
ubiquitin-protein transferase activity
[PMID:17452527|PMID:22085930]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:1326082]"
/experiment="EXISTENCE:direct assay:GO:0006513 protein
monoubiquitination [PMID:17452527|PMID:24034674]"
/experiment="EXISTENCE:direct assay:GO:0051865 protein
autoubiquitination [PMID:17452527]"
/experiment="EXISTENCE:mutant phenotype:GO:0006513 protein
monoubiquitination [PMID:17452527]"
/experiment="EXISTENCE:mutant phenotype:GO:0007031
peroxisome organization [PMID:2568633]"
/experiment="EXISTENCE:mutant phenotype:GO:0016562 protein
import into peroxisome matrix, receptor recycling
[PMID:17452527]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:29519818]"
/note="Peroxisomal ubiquitin conjugating enzyme; required
for peroxisomal matrix protein import and peroxisome
biogenesis"
/codon_start=1
/product="E2 ubiquitin-protein ligase peroxin 4"
/protein_id="NP_011649.1"
/db_xref="GeneID:853034"
/db_xref="SGD:S000003365"
/translation="MPNFWILENRRSYTSDTCMSRIVKEYKVILKTLASDDPIANPYR
GIIESLNPIDETDLSKWEAIISGPSDTPYENHQFRILIEVPSSYPMNPPKISFMQNNI
LHCNVKSATGEICLNILKPEEWTPVWDLLHCVHAVWRLLREPVCDSPLDVDIGNIIRC
GDMSAYQGIVKYFLAERERINNH"
gene <757770..>761138
/gene="CAF130"
/locus_tag="YGR134W"
/db_xref="GeneID:853035"
mRNA <757770..>761138
/gene="CAF130"
/locus_tag="YGR134W"
/product="CCR4-NOT core subunit CAF130"
/transcript_id="NM_001181263.3"
/db_xref="GeneID:853035"
CDS 757770..761138
/gene="CAF130"
/locus_tag="YGR134W"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0032968 positive
regulation of transcription elongation by RNA polymerase
II [PMID:21406554]"
/experiment="EXISTENCE:physical interaction:GO:0030015
CCR4-NOT core complex [PMID:11733989]"
/note="Subunit of the CCR4-NOT transcriptional regulatory
complex; CCR4-NOT complex is evolutionarily-conserved and
involved in controlling mRNA initiation, elongation, and
degradation"
/codon_start=1
/product="CCR4-NOT core subunit CAF130"
/protein_id="NP_011650.3"
/db_xref="GeneID:853035"
/db_xref="SGD:S000003366"
/translation="MTKKKAATNYAERQNLASEDSSGDSVHFKDFIPLQELLKDKNYV
PSVENLEKILYNETMFNDQKICSNLLLEALIITLFTTISGKSALRLIQTSSLKERKSW
AQSFENNSSSYASIVLSWKDNDILLLKFLRFLLANKTAPLQINRYNLPEYKLPLSFLI
VSKITIPSILLNETYNLLKDYLYSITGRIESLISCSSTFDKPALVVRKILKDYNRMIE
CRNFYFWYSFNAENRVNLTFSDNISLLMENDEGNAGSGLDDSRFDHQKQPREAIMGRT
INDQEQIYSFELNQDGTLEIPNVMEHSLLRHELLFKILNLTTVLTPLLELQFSTLCGL
VDPLMQPTPNDKHIISIDFLFQLFLGLMSQSIKTSQEHNDHYDWKFYMCFNMQKIIDA
TMLRLNCFDFDILNSVNNTDNAVHWKTQLHRWLPHGLNTQDLELLYMIDILAVYTIYK
LYEKIPIQLNPFLFSLISLWKNLSCVILLALEIDRIEEENGTYETPLMVRATIRGAAA
LRSVIATVLNGLVKNNDHDFKHESLNTFMSPYGRKLCHGALYADLRSHTASLLALGAS
IEDVTDLFADLQSGDRFDEDIRYMFDYECEDYDESFSESDHGGLDESVVNPTEKIASG
SNNVFFRRRCNCIFNDDKLVAEDGANEAFGSTNSENVEGAMHNNRNAVHNATTATSDH
VVTSPNPLSVRSRSTFEFDYSGEDWRDVPRDFNMYYSPSYSFIHEPKLDVIFSLTLRG
ATEKLNKEESILLVRSVASCVRNEQDQMILADLESNFSASINGDVEGEGNTKMSKIDN
EDLRRTTPDDIYEIWSEESAFERMLNVNHDVAWRLMDEMLMCTGYRRILIWFLTHLEL
KHSLIYYVFELIMGLRGKPFSGEASDQDKKDDMIYEILKKKQKNEDASGLPFSRQGPI
VLSDIETKMLLQEFFMNAAIFLSSKNNEEENEDGEKISLYSLGLVRLICYMVQTLIAN
DKFFFTKSECTFELQTLLMTWIGILPEAKDLFFKIKTRLAMEEEDSADTMQHEGRKNS
DIEKKLNAKPASELNLKLLNLFPSKPANKDDSSPINTLRSFIADYSFDTQVNPPGRRV
VFYDGKILPLPKADKPIPLHEYITLAELDVGDSE"
gene <761392..>762168
/gene="PRE9"
/locus_tag="YGR135W"
/db_xref="GeneID:853036"
mRNA <761392..>762168
/gene="PRE9"
/locus_tag="YGR135W"
/product="proteasome core particle subunit alpha 3"
/transcript_id="NM_001181264.3"
/db_xref="GeneID:853036"
CDS 761392..762168
/gene="PRE9"
/locus_tag="YGR135W"
/experiment="EXISTENCE:direct assay:GO:0010499 proteasomal
ubiquitin-independent protein catabolic process
[PMID:19162040]"
/experiment="EXISTENCE:direct assay:GO:0019773 proteasome
core complex, alpha-subunit complex [PMID:9087403]"
/experiment="EXISTENCE:direct assay:GO:0034515 proteasome
storage granule [PMID:18504300]"
/experiment="EXISTENCE:direct assay:GO:0043161
proteasome-mediated ubiquitin-dependent protein catabolic
process [PMID:11545745|PMID:19029916]"
/experiment="EXISTENCE:mutant phenotype:GO:0080129
proteasome core complex assembly [PMID:14739934]"
/note="Alpha 3 subunit of the 20S proteasome; the only
nonessential 20S subunit; may be replaced by the alpha 4
subunit (Pre6p) under stress conditions to create a more
active proteasomal isoform"
/codon_start=1
/product="proteasome core particle subunit alpha 3"
/protein_id="NP_011651.3"
/db_xref="GeneID:853036"
/db_xref="SGD:S000003367"
/translation="MGSRRYDSRTTIFSPEGRLYQVEYALESISHAGTAIGIMASDGI
VLAAERKVTSTLLEQDTSTEKLYKLNDKIAVAVAGLTADAEILINTARIHAQNYLKTY
NEDIPVEILVRRLSDIKQGYTQHGGLRPFGVSFIYAGYDDRYGYQLYTSNPSGNYTGW
KAISVGANTSAAQTLLQMDYKDDMKVDDAIELALKTLSKTTDSSALTYDRLEFATIRK
GANDGEVYQKIFKPQEIKDILVKTGITKKDEDEEADEDMK"
gene <762428..>763153
/gene="LSB1"
/locus_tag="YGR136W"
/db_xref="GeneID:853037"
mRNA <762428..>763153
/gene="LSB1"
/locus_tag="YGR136W"
/product="Lsb1p"
/transcript_id="NM_001181265.1"
/db_xref="GeneID:853037"
CDS 762428..763153
/gene="LSB1"
/locus_tag="YGR136W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922|PMID:11914276|PMID:23577202|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0030479 actin
cortical patch [PMID:23577202]"
/experiment="EXISTENCE:direct assay:GO:0034316 negative
regulation of Arp2/3 complex-mediated actin nucleation
[PMID:23577202]"
/experiment="EXISTENCE:physical interaction:GO:0034316
negative regulation of Arp2/3 complex-mediated actin
nucleation [PMID:23577202]"
/note="Negative regulator of actin nucleation-promoting
factor activity; interacts with Las17p, a homolog of human
Wiskott-Aldrich Syndrome protein (WASP), via an N-terminal
SH3 domain, and along with PIN3 cooperatively inhibits the
nucleation of actin filaments; overexpression blocks
receptor-mediated endocytosis; protein increases in
abundance and forms nuclear foci in response to DNA
replication stress; LSB1 has a paralog, PIN3, that arose
from the whole genome duplication"
/codon_start=1
/product="Lsb1p"
/protein_id="NP_011652.1"
/db_xref="GeneID:853037"
/db_xref="SGD:S000003368"
/translation="MSASLVNRSLKNIRNELEFLKESNVISGDIFELINSKLPEKWDG
NQRSPQNADTEEYVEALYDFEAQQDGDLSLKTGDKIQVLEKISPDWYRGKSNNKIGIF
PANYVKPAFTRSASPKSAEAASSSTVSRPSVPPPSYEPAASQYPSQQVSAPYAPPAGY
MQAPPPQQQQAPLPYPPPFTNYYQQPQQQYAPPSQQAPVEAQPQQSSGASSAFKSFGS
KLGNAAIFGAGSAIGSDIVNSIF"
gene complement(<763762..>765606)
/gene="TPO2"
/locus_tag="YGR138C"
/db_xref="GeneID:853039"
mRNA complement(<763762..>765606)
/gene="TPO2"
/locus_tag="YGR138C"
/product="spermine transporter"
/transcript_id="NM_001181267.1"
/db_xref="GeneID:853039"
CDS complement(763762..765606)
/gene="TPO2"
/locus_tag="YGR138C"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:12562762]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0000296
spermine transport [PMID:11171066]"
/experiment="EXISTENCE:mutant phenotype:GO:0000297
spermine transmembrane transporter activity
[PMID:11171066]"
/experiment="EXISTENCE:mutant phenotype:GO:0000329
fungal-type vacuole membrane [PMID:11171066]"
/note="Polyamine transporter of the major facilitator
superfamily; member of the 12-spanner drug:H(+) antiporter
DHA1 family; specific for spermine; localizes to the
plasma membrane; transcription of TPO2 is regulated by
Haa1p; TPO2 has a paralog, TPO3, that arose from the whole
genome duplication"
/codon_start=1
/product="spermine transporter"
/protein_id="NP_011654.1"
/db_xref="GeneID:853039"
/db_xref="SGD:S000003370"
/translation="MSDQESVVSFNSQNTSMVDVEGQQPQQYVPSKTNSRANQLKLTK
TETVKSLQDLGVTSAAPVPDINAPQTAKNNIFPEEYTMETPSGLVPVATLQSMGRTAS
ALSRTRTKQLNRTATNSSSTGKEEMEEEETEEREDQSGENELDPEIEFVTFVTGDPEN
PHNWPSWVRWSYTVLLSILVICVAYGSACISGGLGTVEKKYHVGMEAAILSCSLMVIG
FSLGPLIWSPVSDLYGRRVAYFVSMGLYVIFNIPCALAPNLGCLLACRFLCGVWSSSG
LCLVGGSIADMFPSETRGKAIAFFAFAPYVGPVVGPLVNGFISVSTGRMDLIFWVNMA
FAGVMWIISSAIPETYAPVILKRKAARLRKETGNPKIMTEQEAQGVSMSEMMRACLLR
PLYFAVTEPVLVATCFYVCLIYSLLYAFFFAFPVIFGELYGYKDNLVGLMFIPIVIGA
LWALATTFYCENKYLQIVKQRKPTPEDRLLGAKIGAPFAAIALWILGATAYKHIIWVG
PASAGLAFGFGMVLIYYSLNNYIIDCYVQYASSALATKVFLRSAGGAAFPLFTIQMYH
KLNLHWGSWLLAFISTAMIALPFAFSYWGKGLRHKLSKKDYSIDSVEM"
gene <767429..>770299
/gene="CBF2"
/locus_tag="YGR140W"
/gene_synonym="CEP2; CSL5; CTF14; NDC10"
/db_xref="GeneID:853041"
mRNA <767429..>770299
/gene="CBF2"
/locus_tag="YGR140W"
/gene_synonym="CEP2; CSL5; CTF14; NDC10"
/product="Cbf2p"
/transcript_id="NM_001181269.3"
/db_xref="GeneID:853041"
CDS 767429..770299
/gene="CBF2"
/locus_tag="YGR140W"
/gene_synonym="CEP2; CSL5; CTF14; NDC10"
/experiment="EXISTENCE:direct assay:GO:0000779 condensed
chromosome, centromeric region [PMID:9407032]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005819 spindle
[PMID:16381814]"
/experiment="EXISTENCE:direct assay:GO:0008301 DNA
binding, bending [PMID:10097110]"
/experiment="EXISTENCE:direct assay:GO:0019237 centromeric
DNA binding [PMID:9407032]"
/experiment="EXISTENCE:direct assay:GO:0031518 CBF3
complex [PMID:8706132|PMID:8670864|PMID:1997204]"
/experiment="EXISTENCE:direct assay:GO:0051233 spindle
midzone [PMID:12566427]"
/experiment="EXISTENCE:mutant phenotype:GO:0000022 mitotic
spindle elongation [PMID:16381814]"
/experiment="EXISTENCE:mutant phenotype:GO:0000921 septin
ring assembly [PMID:16330709]"
/experiment="EXISTENCE:mutant phenotype:GO:0007059
chromosome segregation [PMID:8486732]"
/note="Essential kinetochore protein; component of the
CBF3 multisubunit complex that binds to the CDEIII region
of the centromere; Cbf2p also binds to the CDEII region
possibly forming a different multimeric complex,
ubiquitinated in vivo; sumoylated in an Mms21p-dependent
manner; relative distribution to the spindle pole body
decreases upon DNA replication stress"
/codon_start=1
/product="Cbf2p"
/protein_id="NP_011656.3"
/db_xref="GeneID:853041"
/db_xref="SGD:S000003372"
/translation="MRSSILFLLKLMKIMDVQQQQEAMSSEDRFQELVDSLKPRTAHQ
YKTYYTKYIQWCQLNQIIPTPEDNSVNSVPYKDLPISAELIHWFLLDTLITDDKPGEK
REETEDLDEEEENSFKIATLKKIIGSLNFLSKLCKVHENPNANIDTKYLESVTKLHTH
WIDSQKAITTNETNNTNTQVLCPPLLKVSLNLWNPETNHLSEKFFKTCSEKLRFLVDF
QLRSYLNLSFEERSKIRFGSLKLGKRDRDAIIYHKVTHSAEKKDTPGHHQLLALLPQD
CPFICPQTTLAAYLYLRFYGIPSVSKGDGFPNLNADENGSLLQDIPILRGKSLTTYPR
EETFSNYYTTVFRYCHLPYKRREYFNKCNLVYPTWDEDTFRTFFNEENHGNWLEQPEA
FAFPDKIPFDFKKIMNFKSPYTSYSTNAKKDPFPPPKDLLVQIFPEIDEYKRHDYEGL
SQNSRDFLDLMEVLRERFLSNLPWIYKFFPNHDIFQDPIFGNSDFQSYFNDKTIHSKG
SPILSFDILPGFNKIYKNKTNFYSLLIERPSQLTFASSHNPDTHPTQKQESEGPLQMS
QLDTTQLNELLKQQSFEYVQFQTLSNFQILLSVFNKIFEKLEMKKSSRGYILHQLNLF
KITLDERIKKSKIDDADKFIRDNQPIKKEENIVNEDGPNTSRRTKRPKQIRLLSIADS
SDESSTEDSNVFKKDGESIEDGAYGENEDENDSEMQEQLKSMINELINSKISTFLRDQ
MDQFELKINALLDKILEEKVTRIIEQKLGSHTGKFSTLKRPQLYMTEEHNVGFDMEVP
KKLRTSGKYAETVKDNDDHQAMSTTASPSPEQDQEAKSYTDEQEFMLDKSIDSIEGII
LEWFTPNAKYANQCVHSMNKSGNKSWRANCEALYKERKSIVEFYIYLVNHESLDRYKA
VDICEKLRDQNEGSFSRLAKFLRKWRHDHQNSFDGLLVYLSN"
gene <770569..>771972
/gene="VPS62"
/locus_tag="YGR141W"
/db_xref="GeneID:853042"
mRNA <770569..>771972
/gene="VPS62"
/locus_tag="YGR141W"
/product="Vps62p"
/transcript_id="NM_001181270.1"
/db_xref="GeneID:853042"
CDS 770569..771972
/gene="VPS62"
/locus_tag="YGR141W"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:19001347]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0006623 protein
targeting to vacuole [PMID:12134085]"
/note="Vacuolar protein sorting (VPS) protein; required
for cytoplasm to vacuole targeting of proteins; targeted
to vacuole via AP-3 pathway; VPS62 has a paralog, TDA6,
that arose from the whole genome duplication"
/codon_start=1
/product="Vps62p"
/protein_id="NP_011657.1"
/db_xref="GeneID:853042"
/db_xref="SGD:S000003373"
/translation="MRISKNSHKRQRTRLYFLVTFIIYSIIPCRAVLVPWLDDDPFEA
TLLEMGDEPWSKDILSSTPPLHPSEVTEDNKSLKQRGNVPQYVIDNSPLLHLYSEEKY
WPADVKDFVKRFQLRDHSGEKIINEHLRDLSDLQEYYSVELENGTWGRVSSEGTYMTS
LDDFDKGPDWLLGEQPEYGTGHIKKAPAVLFVVDKGNGWVDAFWFYFYPFNWGPYIMG
SGPWGNHVGDWEHSLVRFYKGEPQYLWMSAHGGGSAYKFEAIEKIKRLRRVDGKLTNE
VIKKPLIFSARGTHAHYASVGQHAHDVPFFFMPLSDFTDRGPLWDPSLNYYAYTVTVG
EKMTPCGAEETKMGLEWLSFKGAWGDKQLRPRDPRQKWCPFQWKYIDGPKGPLFKNME
RVSLCQRFKWWNFWKGCPARRYIKRGEGLDAEKNDLVGDNCGILLYNIRPKWLRSILR
FLTWRGSVCFIMDYFTG"
gene <772454..>773686
/gene="BTN2"
/locus_tag="YGR142W"
/db_xref="GeneID:853043"
mRNA <772454..>773686
/gene="BTN2"
/locus_tag="YGR142W"
/product="Btn2p"
/transcript_id="NM_001181271.3"
/db_xref="GeneID:853043"
CDS 772454..773686
/gene="BTN2"
/locus_tag="YGR142W"
/experiment="EXISTENCE:direct assay:GO:0000149 SNARE
binding [PMID:17101785]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22718905]"
/experiment="EXISTENCE:direct assay:GO:0005770 late
endosome [PMID:17101785]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22718905|PMID:11053386]"
/experiment="EXISTENCE:direct assay:GO:0034399 nuclear
periphery [PMID:25817432]"
/experiment="EXISTENCE:direct assay:GO:0048471 perinuclear
region of cytoplasm [PMID:18833194]"
/experiment="EXISTENCE:genetic interaction:GO:0006457
protein folding [PMID:22718905]"
/experiment="EXISTENCE:mutant phenotype:GO:0006457 protein
folding [PMID:22718905]"
/experiment="EXISTENCE:mutant phenotype:GO:0006865 amino
acid transport [PMID:15701790]"
/experiment="EXISTENCE:mutant phenotype:GO:0006885
regulation of pH [PMID:15701790|PMID:11053386]"
/experiment="EXISTENCE:mutant phenotype:GO:0006886
intracellular protein transport
[PMID:22718905|PMID:12615067]"
/experiment="EXISTENCE:mutant phenotype:GO:0034504 protein
localization to nucleus [PMID:22718905]"
/experiment="EXISTENCE:mutant phenotype:GO:0042147
retrograde transport, endosome to Golgi [PMID:17101785]"
/experiment="EXISTENCE:mutant phenotype:GO:0140311 protein
sequestering activity [PMID:31649258]"
/experiment="EXISTENCE:physical interaction:GO:0000149
SNARE binding [PMID:17101785]"
/experiment="EXISTENCE:physical interaction:GO:0006457
protein folding [PMID:22718905]"
/experiment="EXISTENCE:physical interaction:GO:0006886
intracellular protein transport [PMID:22718905]"
/experiment="EXISTENCE:physical interaction:GO:0034504
protein localization to nucleus [PMID:22718905]"
/experiment="EXISTENCE:physical interaction:GO:0051087
protein-folding chaperone binding [PMID:22718905]"
/note="Compartment-specific sequestrase chaperone; small
hsp that sequesters proteins at intranuclear quality
control sites (INQs); sumoylated form promotes protein
clearance from INQs during recovery from genotoxic stress;
v-SNARE binding protein that mediates late endosome-Golgi
protein sorting; modulates arginine uptake and pH
homeostasis between the vacuole and plasma membrane
H(+)-ATPase; role in prion curing; expression after severe
ethanol stress is involved in clearance of denatured
proteins"
/codon_start=1
/product="Btn2p"
/protein_id="NP_011658.3"
/db_xref="GeneID:853043"
/db_xref="SGD:S000003374"
/translation="MFSIFNSPCVFEQLPSFSQPLHSRYFDCSSPVSYYPECKRRKAI
KANLRAPKKSDANCSEPLRYALAETPNGYTLSLSKRIPYELFSKYVNEKLGELKENHY
RPTYHVVQDFFGNQYYVEDEADEDALLRSALKDLDFRAIGKKIAKDLFQDYEIELNHR
GDELSILSKKDKIFKEFSLDQVFEDVFVIGCGVENIDDGSREKYALLKIGLVKHEEEI
SEGGINEPKMPIIESKIDESHDDVNMSESLKEEEAEKAKEPLTKEDQIKKWIEEERLM
QEESRKSEQEKAAKEDEERQKKEKEARLKARKESLINKQKTKRSQQKKLQNSKSLPIS
EIEASNKNNNSNSGSAESDNESINSDSDTTLDFSVSGNTLKKHASPLLEDVEDEEVDR
YNESLSRSPKGNSIIEEI"
repeat_region 774040..774162
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006970"
gene 774349..774421
/locus_tag="YNCG0034W"
/db_xref="GeneID:853044"
tRNA 774349..774421
/locus_tag="YNCG0034W"
/product="tRNA-Ala"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE
analysis; one of 11 nuclear tRNA genes containing the
tDNA-anticodon AGC (converted to IGC in the mature tRNA),
decodes GCU and GCC codons into alanine, one of 16 nuclear
tRNAs for alanine"
/db_xref="GeneID:853044"
/db_xref="SGD:S000006512"
gene <775193..>777508
/gene="SKN1"
/locus_tag="YGR143W"
/db_xref="GeneID:853045"
mRNA <775193..>777508
/gene="SKN1"
/locus_tag="YGR143W"
/product="beta-glucan synthesis-associated protein SKN1"
/transcript_id="NM_001181272.3"
/db_xref="GeneID:853045"
CDS 775193..777508
/gene="SKN1"
/locus_tag="YGR143W"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:7929594]"
/experiment="EXISTENCE:genetic interaction:GO:0010507
negative regulation of autophagy [PMID:20030721]"
/experiment="EXISTENCE:genetic interaction:GO:0030148
sphingolipid biosynthetic process [PMID:15792805]"
/experiment="EXISTENCE:mutant phenotype:GO:0006078
(1->6)-beta-D-glucan biosynthetic process [PMID:7929594]"
/experiment="EXISTENCE:mutant phenotype:GO:0015926
glucosidase activity [PMID:10601196]"
/experiment="EXISTENCE:mutant phenotype:GO:0030148
sphingolipid biosynthetic process [PMID:15792805]"
/experiment="EXISTENCE:mutant phenotype:GO:0031505
fungal-type cell wall organization [PMID:7929594]"
/note="Protein involved in sphingolipid biosynthesis; type
II membrane protein; SKN1 has a paralog, KRE6, that arose
from the whole genome duplication"
/codon_start=1
/product="beta-glucan synthesis-associated protein SKN1"
/protein_id="NP_011659.3"
/db_xref="GeneID:853045"
/db_xref="SGD:S000003375"
/translation="MSVRNLTNNRHSNSENSVSGSENSFYSSNEQSRQSSSLEPADGQ
NVRVSGNPFLGSEEFDEDYNSPSGDDERRGANEYSSSSSINYNNDPNSDTSLLANEKN
SPERNGQRMSDYKGYYAKTNLTSANNLNNHNNNNYKNIISSSNDNSFASHLQPPDRNL
PSHPSSNNMSSFSNNSLIKSPPPFDRYPLVGTRHISAAQSQSQNLINEKKRANMTGSS
SSAHDSSLSSTNLYMGEQDFSPFGGYPASFFPLTLDEKEDDDYIHNPNVEEEAKLDRR
RFVDDFKHMDRRSFLGLLGILFLFMAGIFIFIVLPAITFSGVVYHHEHVHAANSAGSS
SSNTTSKSLTEYQYPQLAAIRTTLVDPDTPDSAKTRVAKDGSKWQLVFSDEFNAEGRT
FYDGDDQFWTAPDIHYDATKDLEWYSPDAVTTTNGTLTLRMDAFRNHDLYYRSGMVQS
WNKLCFTEGALEVSANLPNYGRVTGLWPGMWTMGNLGRPGYLASTQGVWPYSYEACDA
GITPNQSSPDGISYLPGQKLSVCTCDNEDHPNQGVGRGAPEIDILEGEADTILGVGVA
SQSLQIAPFDIWYMPDYDFIEVYNFTTTTMNTYAGGPFQQAVSAISTLNVTWYEFGEE
AGYFQKYAIEYLNDDDNGYIRWFVGENPTFTLYATSLHPSGNIDWRRISKEPMSAILN
LGISNNWAYIDWQYIFFPVTMSIDYVRLYQPKGSTSITCDPEDYPTYDYIQSHLNAYY
NANLTDWEQAGYTFPKNILTGGCSSSKFSLS"
rep_origin 777966..778214
/note="ARS729; Autonomously Replicating Sequence"
/db_xref="SGD:S000118465"
repeat_region 778784..779112
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006971"
repeat_region 779116..779224
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006972"
repeat_region complement(779225..779515)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006958"
gene 779616..779687
/gene="SUF4"
/locus_tag="YNCG0035W"
/db_xref="GeneID:853046"
tRNA 779616..779687
/gene="SUF4"
/locus_tag="YNCG0035W"
/product="tRNA-Gly"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE
analysis; can mutate to suppress +1 frameshift mutations
in glycine codons"
/db_xref="GeneID:853046"
/db_xref="SGD:S000006590"
gene <780399..>781379
/gene="THI4"
/locus_tag="YGR144W"
/gene_synonym="ESP35; MOL1"
/db_xref="GeneID:853047"
mRNA <780399..>781379
/gene="THI4"
/locus_tag="YGR144W"
/gene_synonym="ESP35; MOL1"
/product="thiamine thiazole synthase"
/transcript_id="NM_001181273.1"
/db_xref="GeneID:853047"
CDS 780399..781379
/gene="THI4"
/locus_tag="YGR144W"
/gene_synonym="ESP35; MOL1"
/EC_number="2.4.2.60"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:16850348|PMID:15544818]"
/experiment="EXISTENCE:direct assay:GO:0008198 ferrous
iron binding [PMID:22031445]"
/experiment="EXISTENCE:direct assay:GO:0052837 thiazole
biosynthetic process [PMID:22031445]"
/experiment="EXISTENCE:direct assay:GO:0160205
cysteine-dependent adenosine diphosphate thiazole synthase
activity [PMID:22031445]"
/experiment="EXISTENCE:mutant phenotype:GO:0009228
thiamine biosynthetic process [PMID:7941734]"
/note="Thiazole synthase; abundant protein involved in the
formation of the thiazole moiety of thiamine during
thiamine biosynthesis; acts more as a co-substrate rather
than an enzyme by providing the sulphur source for
thiazole formation; undergoes a single turnover only;
required for mitochondrial genome stability in response to
DNA damaging agents"
/codon_start=1
/product="thiamine thiazole synthase"
/protein_id="NP_011660.1"
/db_xref="GeneID:853047"
/db_xref="SGD:S000003376"
/translation="MSATSTATSTSASQLHLNSTPVTHCLSDIVKKEDWSDFKFAPIR
ESTVSRAMTSRYFKDLDKFAVSDVIIVGAGSSGLSAAYVIAKNRPDLKVCIIESSVAP
GGGSWLGGQLFSAMVMRKPAHLFLQELEIPYEDEGDYVVVKHAALFISTVLSKVLQLP
NVKLFNATCVEDLVTRPPTEKGEVTVAGVVTNWTLVTQAHGTQCCMDPNVIELAGYKN
DGTRDLSQKHGVILSTTGHDGPFGAFCAKRIVDIDQNQKLGGMKGLDMNHAEHDVVIH
SGAYAGVDNMYFAGMEVAELDGLNRMGPTFGAMALSGVHAAEQILKHFAA"
gene <781767..>783890
/gene="ENP2"
/locus_tag="YGR145W"
/db_xref="GeneID:853048"
mRNA <781767..>783890
/gene="ENP2"
/locus_tag="YGR145W"
/product="ribosome biosynthesis protein ENP2"
/transcript_id="NM_001181274.2"
/db_xref="GeneID:853048"
CDS 781767..783890
/gene="ENP2"
/locus_tag="YGR145W"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:14690591|PMID:15590835]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/experiment="EXISTENCE:mutant phenotype:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:24357410]"
/experiment="EXISTENCE:mutant phenotype:GO:0032040
small-subunit processome [PMID:24357410]"
/experiment="EXISTENCE:mutant phenotype:GO:0042274
ribosomal small subunit biogenesis [PMID:19806183]"
/note="Component of the SSU; required for pre-18S rRNA
processing, biogenesis of the small ribosomal subunit;
interacts with U3 snoRNA, Mpp10p and Bfr2p; contains WD
repeats, and has homology to Spb1p"
/codon_start=1
/product="ribosome biosynthesis protein ENP2"
/protein_id="NP_011661.2"
/db_xref="GeneID:853048"
/db_xref="SGD:S000003377"
/translation="MVLKSTSANDVSVYQVSGTNVSRSLPDWIAKKRKRQLKNDLEYQ
NRVELIQDFEFSEASNKIKVSRDGQYCMATGTYKPQIHVYDFANLSLKFDRHTDAENV
DFTILSDDWTKSVHLQNDRSIQFQNKGGLHYTTRIPKFGRSLVYNKVNCDLYVGASGN
ELYRLNLEKGRFLNPFKLDTEGVNHVSINEVNGLLAAGTETNVVEFWDPRSRSRVSKL
YLENNIDNRPFQVTTTSFRNDGLTFACGTSNGYSYIYDLRTSEPSIIKDQGYGFDIKK
IIWLDNVGTENKIVTCDKRIAKIWDRLDGKAYASMEPSVDINDIEHVPGTGMFFTANE
SIPMHTYYIPSLGPSPRWCSFLDSITEELEEKPSDTVYSNYRFITRDDVKKLNLTHLV
GSRVLRAYMHGFFINTELYDKVSLIANPDAYKDEREREIRRRIEKERESRIRSSGAVQ
KPKIKVNKTLVDKLSQKRGDKVAGKVLTDDRFKEMFEDEEFQVDEDDYDFKQLNPVKS
IKETEEGAAKRIRALTAAEESDEERIAMKDGRGHYDYEDEESDEEESDDETNQKSNKE
ELSEKDLRKMEKQKALIERRKKEKEQSERFMNEMKAGTSTSTQRDESAHVTFGEQVGE
LLEVENGKKSNESILRRNQRGEAELTFIPQRKSKKDGNYKSRRHDNSSDEEGIDENGN
KKDNGRSKPRFENRRRASKNAFRGM"
gene complement(<784223..>784858)
/gene="ECL1"
/locus_tag="YGR146C"
/db_xref="GeneID:853049"
mRNA complement(<784223..>784858)
/gene="ECL1"
/locus_tag="YGR146C"
/product="Ecl1p"
/transcript_id="NM_001181275.3"
/db_xref="GeneID:853049"
CDS complement(784223..784858)
/gene="ECL1"
/locus_tag="YGR146C"
/note="hypothetical protein; mitochondrial-dependent role
in the extension of chronological lifespan; overexpression
increases oxygen consumption and respiratory activity
while deletion results in reduced oxygen consumption under
conditions of caloric restriction; induced by iron
homeostasis transcription factor Aft2p; multicopy
suppressor of temperature sensitive hsf1 mutant; induced
by treatment with 8-methoxypsoralen and UVA irradiation"
/codon_start=1
/product="Ecl1p"
/protein_id="NP_011662.3"
/db_xref="GeneID:853049"
/db_xref="SGD:S000003378"
/translation="MSTAFNDYCTVCDRLIPTSPQKTNINTRKIQRDNETKSSLQSNK
LYCSEDCKLKDSNPLNEKLLSHLHKKSKTSHSHNLTPPLSYSKNLTASNLFEPTTSLS
SSPTSSTIPFDELEKLESLLISPLLLPQDGIVNPKQESNPSRVDEYDENEHYLNLADS
LRLDSSYQLHSKAHLGYENNLPRSNDLIDDHLISDQIIENNYNLWFRLSSS"
gene complement(<785276..>785437)
/locus_tag="YGR146C-A"
/db_xref="GeneID:1466459"
mRNA complement(<785276..>785437)
/locus_tag="YGR146C-A"
/product="uncharacterized protein"
/transcript_id="NM_001184594.1"
/db_xref="GeneID:1466459"
CDS complement(785276..785437)
/locus_tag="YGR146C-A"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878079.1"
/db_xref="GeneID:1466459"
/db_xref="SGD:S000028638"
/translation="MGFLPECNLTCAFLLHSFTFPIAHCPSFSWASFFFTIRPPFFPK
LALVCTIFS"
gene complement(<786054..>786920)
/gene="NAT2"
/locus_tag="YGR147C"
/db_xref="GeneID:853050"
mRNA complement(<786054..>786920)
/gene="NAT2"
/locus_tag="YGR147C"
/product="Nat2p"
/transcript_id="NM_001181276.3"
/db_xref="GeneID:853050"
CDS complement(786054..786920)
/gene="NAT2"
/locus_tag="YGR147C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:26928762|PMID:16823961|PMID:14576278|PMID:24769239]"
/experiment="EXISTENCE:mutant phenotype:GO:0017196
N-terminal peptidyl-methionine acetylation [PMID:8175741]"
/note="hypothetical protein; has an apparent role in
acetylation of N-terminal methionine residues"
/codon_start=1
/product="Nat2p"
/protein_id="NP_011663.3"
/db_xref="GeneID:853050"
/db_xref="SGD:S000003379"
/translation="MMVPRISASPVFKRIFLRWGFVTLPIQKTVSHTLRRDFSAPCRS
MVKCLLLRPGISVHSAQDRKFYSTEEKSSQFDENKSKSNNGKKNEPHGIKGLMAKYGY
SALIVYILLTCVDLPLCFLGVHSLGEEKIKIYLNRGKQLIGMGEPDESKVIQDVRRKQ
AHREAVQAENADKVEDASRKTFNERWQEMKDSTLLAELLIAYGIHKSLIIVRVPLTAV
LTPSFVKLLQRFGIDLMKKQKKVFQTMASGAKIRYKGNNPSDFIKNEGTALDITKRKP
RTKGQKWFDGLM"
gene complement(<787312..>787779)
/gene="RPL24B"
/locus_tag="YGR148C"
/gene_synonym="RPL30B"
/db_xref="GeneID:853051"
mRNA complement(<787312..>787779)
/gene="RPL24B"
/locus_tag="YGR148C"
/gene_synonym="RPL30B"
/product="60S ribosomal protein eL24 RPL24B"
/transcript_id="NM_001181277.3"
/db_xref="GeneID:853051"
CDS complement(787312..787779)
/gene="RPL24B"
/locus_tag="YGR148C"
/gene_synonym="RPL30B"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:6337137]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:11983894]"
/experiment="EXISTENCE:mutant phenotype:GO:0006413
translational initiation [PMID:30407570]"
/experiment="EXISTENCE:mutant phenotype:GO:0006414
translational elongation [PMID:30407570]"
/note="Ribosomal 60S subunit protein L24B; forms two
bridges within ribosome, stimulates translation initiation
and elongation; homologous to mammalian ribosomal protein
L24, no bacterial homolog; RPL24B has a paralog, RPL24A,
that arose from the whole genome duplication"
/codon_start=1
/product="60S ribosomal protein eL24 RPL24B"
/protein_id="NP_011664.3"
/db_xref="GeneID:853051"
/db_xref="SGD:S000003380"
/translation="MKVEVDSFSGAKIYPGRGTLFVRGDSKIFRFQNSKSASLFKQRK
NPRRIAWTVLFRKHHKKGITEEVAKKRSRKTVKAQRPITGASLDLIKERRSLKPEVRK
ANREEKLKANKEKKRAEKAARKAEKAKSAGVQGSKVSKQQAKGAFQKVAATSR"
gene <789031..>790329
/gene="GPC1"
/locus_tag="YGR149W"
/db_xref="GeneID:853052"
mRNA <789031..>790329
/gene="GPC1"
/locus_tag="YGR149W"
/product="glycerophosphocholine acyltransferase"
/transcript_id="NM_001181278.1"
/db_xref="GeneID:853052"
CDS 789031..790329
/gene="GPC1"
/locus_tag="YGR149W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0006656
phosphatidylcholine biosynthetic process [PMID:27758859]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0106158
glycero-3-phosphocholine acyltransferase activity
[PMID:27758859]"
/experiment="EXISTENCE:genetic interaction:GO:0090640
phosphatidylcholine biosynthesis from
sn-glycero-3-phosphocholine [PMID:30514764]"
/experiment="EXISTENCE:mutant phenotype:GO:0036151
phosphatidylcholine acyl-chain remodeling [PMID:30514764]"
/note="Glycerophosphocholine acyltransferase (GPCAT);
involved in in phosphatidylcholine (PC) synthesis; uses
acyl-CoA to acylate glycero-3-phosphocholine to yield
lyso-PC; also catalyzes acylation of
glycerophosphoethanolamine with acyl-CoA; predicted to be
an integral membrane protein"
/codon_start=1
/product="glycerophosphocholine acyltransferase"
/protein_id="NP_011665.1"
/db_xref="GeneID:853052"
/db_xref="SGD:S000003381"
/translation="MYKLDNNDIDDETNNSVSLTSLLEFLDPIASKVVSKYYHGSHLS
KAEQKLRNFEGFRRRKPHHEHDSHHPHHLNRSRSFLQLEDFKVRALQRIRNLDKPLDS
IFFKNSSRLEKAFYPFTLFNIFFIGFLMGRFPEWFHVYYTILFFVLMPIRFYTYYKTK
NHYFLADFCYFVNMLCLLFIWIFPYSYSLFQSCFAFTFGTLCFAVITWRNSLVIHSID
KTTSCFIHIIPPCVMYVIYHGLPLEYKIERFPGAIIQSELDIKKNILWTSLYYLVWQS
LYHYFITLKKSSKIKSGERMTSFEYLTTHQFKNFWAVKLRSPWPMIIYTLSQYFYQLF
TMLLCGIWIRYKLAAALFLTIVFLWASHNGATYYIDHYGKNFEKEVDRLRLEVENLQQ
KLQPDSDAVISDASVNDKDYLNVNRDEDFDDSSSVSSKSD"
gene complement(<790459..>793053)
/gene="CCM1"
/locus_tag="YGR150C"
/gene_synonym="DMR1; RRG2"
/db_xref="GeneID:853053"
mRNA complement(<790459..>793053)
/gene="CCM1"
/locus_tag="YGR150C"
/gene_synonym="DMR1; RRG2"
/product="Ccm1p"
/transcript_id="NM_001181279.1"
/db_xref="GeneID:853053"
CDS complement(790459..793053)
/gene="CCM1"
/locus_tag="YGR150C"
/gene_synonym="DMR1; RRG2"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:16823961|PMID:14562095|PMID:24769239|PMID:20124025]"
/experiment="EXISTENCE:direct assay:GO:0019843 rRNA
binding [PMID:20124025]"
/experiment="EXISTENCE:mutant phenotype:GO:0000963
mitochondrial RNA processing [PMID:19562342]"
/experiment="EXISTENCE:mutant phenotype:GO:0008380 RNA
splicing [PMID:19562342]"
/experiment="EXISTENCE:mutant phenotype:GO:0016072 rRNA
metabolic process [PMID:20124025]"
/experiment="EXISTENCE:mutant phenotype:GO:2000234
positive regulation of rRNA processing [PMID:32152734]"
/note="Mitochondrial 15S rRNA-binding protein; required
for intron removal of COB and COX1 pre-mRNAs; has
separable roles in stabilizing mitochondrial 15S rRNA and
in maturation of the COB and COX1 mRNAs; contains
pentatricopeptide repeat (PPR) motifs; mutant is
respiratory deficient and has defective plasma membrane
electron transport"
/codon_start=1
/product="Ccm1p"
/protein_id="NP_011666.1"
/db_xref="GeneID:853053"
/db_xref="SGD:S000003382"
/translation="MYMARCGPKNNVLCFPFQLSFLFSKRLINKRFKYTLQTEDEKNM
MGSLSKNKIITPEDVEFKLAQLREFSNTLKERIHNTKSVNSDGHQSNSIAPISEDSRN
VNVTKTSSVPNEEKSKNLSDLIHSSFLEKMDHLVPKVIRERVADDDILAKNLFDRSHS
NWAPVIDRLYVSEKRFMDIDSREFSVWLNGTVKYLPFHSILHLDEMLLEQINGDVVKF
NTHMYECIFNNLGNLKPTNFNQDGTNDKVILKMKELLERYDKALKITEERINKKEGFP
SKVPKMTQAILNNCLKYSTKCSSFHDMDYFITKFRDDYGITPNKQNLTTVIQFYSRKE
MTKQAWNTFDTMKFLSTKHFPDICTYNTMLRICEKERNFPKALDLFQEIQDHNIKPTT
NTYIMMARVLASSSSNAVVSEGKSDSLRLLGWKYLHELEDKNLYRHKKDDLNLFLAMM
ALAAFDGDIELSRALYYLFIAKKYKTLCANWKGNILVDQDTIWKSTLMPEMLNYLMLA
YARFDPRNLPVLSGYEKGIELRRKFLREFDSSMRLDDTDKLVKFKLPFLPISDLNSEA
QVLAESNAIWSFNMENGGTRNTLTSSNEAALEDIKKYRQLLDSFAQEAEDFNEFKFKV
MYEVTKMQRESINVNVFNKISLHTYLSIPINLKQQKEFLRRLTFFTFQQHEFEAVIKR
LYEGYRNIPSSHTRDQNSISTEAISVSKPETTEDLNLIMHDIWYITCLRHKIMMDTTL
YELVMKAAIEFQNEDLAKKVWNDRGKFRTTVPFLKMDQRIRIAKDQKFAHLMVEFFTK
QGKYSDAIAIILSSKNRFNWTYSMVRNLHKALEEIEDRNSVEILLDVVNKKSHAKALK
WEEQELNM"
repeat_region 793237..793559
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006973"
gene 794417..794489
/locus_tag="YNCG0036W"
/db_xref="GeneID:853054"
tRNA 794417..794489
/locus_tag="YNCG0036W"
/product="tRNA-Ala"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE
analysis; one of 5 nuclear tRNA genes containing the
tDNA-anticodon TGC (mature tRNA may be UGC or may contain
modified bases), decodes GCA and probably GCG codons into
alanine, one of 16 nuclear tRNAs for alanine"
/db_xref="GeneID:853054"
/db_xref="SGD:S000006523"
gene complement(<794674..>795492)
/gene="RSR1"
/locus_tag="YGR152C"
/gene_synonym="BUD1"
/db_xref="GeneID:853056"
mRNA complement(<794674..>795492)
/gene="RSR1"
/locus_tag="YGR152C"
/gene_synonym="BUD1"
/product="Ras family GTPase RSR1"
/transcript_id="NM_001181281.3"
/db_xref="GeneID:853056"
CDS complement(794674..795492)
/gene="RSR1"
/locus_tag="YGR152C"
/gene_synonym="BUD1"
/EC_number="3.6.5.2"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:12058023]"
/experiment="EXISTENCE:direct assay:GO:0003924 GTPase
activity [PMID:1910037]"
/experiment="EXISTENCE:direct assay:GO:0005774 vacuolar
membrane [PMID:12058023]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:8723349|PMID:12058023]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:15282802]"
/experiment="EXISTENCE:direct assay:GO:0030427 site of
polarized growth [PMID:12058023]"
/experiment="EXISTENCE:direct assay:GO:0032153 cell
division site [PMID:12058023]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0030427 site
of polarized growth [PMID:14872283]"
/experiment="EXISTENCE:genetic interaction:GO:0032507
maintenance of protein location in cell [PMID:14872283]"
/experiment="EXISTENCE:mutant phenotype:GO:0000080 mitotic
G1 phase [PMID:12058023]"
/experiment="EXISTENCE:mutant phenotype:GO:0000131
incipient cellular bud site [PMID:14988726|PMID:12058023]"
/experiment="EXISTENCE:mutant phenotype:GO:0000755
cytogamy [PMID:9744878]"
/experiment="EXISTENCE:mutant phenotype:GO:0007120 axial
cellular bud site selection [PMID:20587777]"
/experiment="EXISTENCE:mutant phenotype:GO:0007121 bipolar
cellular bud site selection [PMID:2065354]"
/experiment="EXISTENCE:mutant phenotype:GO:0007264 small
GTPase-mediated signal transduction [PMID:1910037]"
/experiment="EXISTENCE:mutant phenotype:GO:0035025
positive regulation of Rho protein signal transduction
[PMID:14988726]"
/experiment="EXISTENCE:mutant phenotype:GO:0045184
establishment of protein localization
[PMID:14988726|PMID:12058023]"
/experiment="EXISTENCE:mutant phenotype:GO:2000114
regulation of establishment of cell polarity
[PMID:31411940]"
/note="Ras-type GTPase; required for bud site selection
during both axial and bipolar budding, for morphological
changes in response to mating pheromone, and for efficient
cell fusion; recruits Cdc24p to the incipient bud site,
activating its guanyl-nucleotide exchange factor activity
and the Rho-like GTPase, Cdc42p; localizes to the plasma
membrane, the incipient bud site, polarized growth sites,
the cell division site and the vacuole membrane; Ras
superfamily member similar to mammalian Rap GTPases"
/codon_start=1
/product="Ras family GTPase RSR1"
/protein_id="NP_011668.3"
/db_xref="GeneID:853056"
/db_xref="SGD:S000003384"
/translation="MRDYKLVVLGAGGVGKSCLTVQFVQGVYLDTYDPTIEDSYRKTI
EIDNKVFDLEILDTAGIAQFTAMRELYIKSGMGFLLVYSVTDRQSLEELMELREQVLR
IKDSDRVPMVLIGNKADLINERVISVEEGIEVSSKWGRVPFYETSALLRSNVDEVFVD
LVRQIIRNEMESVAVKDARNQSQQFSKIESPSTRLPSSAKQDTKQSNNKQSSKGLYNK
SSQGQAKVKQSTPVNEKHKPSHAVPKSGSSNRTGISATSQQKKKKKNASTCTIL"
gene <796092..>796745
/locus_tag="YGR153W"
/db_xref="GeneID:853057"
mRNA <796092..>796745
/locus_tag="YGR153W"
/product="uncharacterized protein"
/transcript_id="NM_001181282.2"
/db_xref="GeneID:853057"
CDS 796092..796745
/locus_tag="YGR153W"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011669.2"
/db_xref="GeneID:853057"
/db_xref="SGD:S000003385"
/translation="MGKACLNKEVGTYECEGERDTYSFFTSLSDIQDSSSNEEQCGVG
SILSEDSFTFEGSNVSIRLFSLDLNALNENENGSKNPVKFTIPPKIEQRKEARQREKR
LRRVAVLPENSRNYLVESSMDSSREYSQPFFDWRHEMVEHGEESVKPCGCHKSRKAKC
FKELEMENIEKGDIKKSLFYRDIIEWCRDYEVNKTREVCVPSIHEFYLHGNGSDNLF"
gene complement(<796798..>797868)
/gene="GTO1"
/locus_tag="YGR154C"
/db_xref="GeneID:853058"
mRNA complement(<796798..>797868)
/gene="GTO1"
/locus_tag="YGR154C"
/product="omega-class glutathione transferase"
/transcript_id="NM_001181283.3"
/db_xref="GeneID:853058"
CDS complement(796798..797868)
/gene="GTO1"
/locus_tag="YGR154C"
/EC_number="1.8.5.1"
/EC_number="2.5.1.18"
/experiment="EXISTENCE:direct assay:GO:0004364 glutathione
transferase activity [PMID:16709151]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:16936141]"
/note="Omega-class glutathione transferase; induced under
oxidative stress; putative peroxisomal localization"
/codon_start=1
/product="omega-class glutathione transferase"
/protein_id="NP_011670.3"
/db_xref="GeneID:853058"
/db_xref="SGD:S000003386"
/translation="MSVSYKGTISKTHSVFKPEKGRYYIYGALGCPFTHRAILARSLK
KLEPVLGLVLSHWQLDSKGARFLPAPHRPEKYKERFFTATGGIASAKLDESEELGDVN
NDSARLFVDGAFDPVENISRLSELYYLNDPKYPGTKFTVPVLWDSKTRKIVNNESGDI
IRILNSGVFDEFIQSEETNVIDLVPHDLIDEIDKNIKWVHPKINLGVYKVGLAENGKI
YETEVKTLFENLQKMECVLKENYKRLEEQFSGNKQKILAKYFVLGQRLTEADIRLYPS
IIRFDVVYVQHFKCNLKTIRDGFPYLHLWLINLYWNYAEFRFTTDFNHIKLFYIRMEV
SRNKINQFGIVPLGPKPDISRL"
gene <798543..>800066
/gene="CYS4"
/locus_tag="YGR155W"
/gene_synonym="NHS5; STR4; VMA41"
/db_xref="GeneID:853059"
mRNA <798543..>800066
/gene="CYS4"
/locus_tag="YGR155W"
/gene_synonym="NHS5; STR4; VMA41"
/product="cystathionine beta-synthase CYS4"
/transcript_id="NM_001181284.3"
/db_xref="GeneID:853059"
CDS 798543..800066
/gene="CYS4"
/locus_tag="YGR155W"
/gene_synonym="NHS5; STR4; VMA41"
/EC_number="4.2.1.22"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0004122
cystathionine beta-synthase activity
[PMID:10766767|PMID:11948191|PMID:19264153]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:23222640|PMID:11914276|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0006535 L-cysteine
biosynthetic process from L-serine [PMID:11948191]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405]"
/experiment="EXISTENCE:direct assay:GO:0070814 hydrogen
sulfide biosynthetic process [PMID:19531479]"
/experiment="EXISTENCE:mutant phenotype:GO:0004122
cystathionine beta-synthase activity
[PMID:8366024|PMID:10509018]"
/experiment="EXISTENCE:mutant phenotype:GO:0007089
traversing start control point of mitotic cell cycle
[PMID:22438835]"
/experiment="EXISTENCE:mutant phenotype:GO:0019343
L-cysteine biosynthetic process via L-cystathionine
[PMID:10509018|PMID:8366024]"
/experiment="EXISTENCE:mutant phenotype:GO:0019346
transsulfuration [PMID:8366024]"
/note="Cystathionine beta-synthase; catalyzes synthesis of
cystathionine from serine and homocysteine, the first
committed step in cysteine biosynthesis; responsible for
hydrogen sulfide generation; advances passage through
START by promoting cell growth which requires catalytic
activity, and reducing critical cell size independent of
catalytic activity; mutations in human ortholog CBS cause
homocystinuria; human CBS can complement yeast null
mutant"
/codon_start=1
/product="cystathionine beta-synthase CYS4"
/protein_id="NP_011671.3"
/db_xref="GeneID:853059"
/db_xref="SGD:S000003387"
/translation="MTKSEQQADSRHNVIDLVGNTPLIALKKLPKALGIKPQIYAKLE
LYNPGGSIKDRIAKSMVEEAEASGRIHPSRSTLIEPTSGNTGIGLALIGAIKGYRTII
TLPEKMSNEKVSVLKALGAEIIRTPTAAAWDSPESHIGVAKKLEKEIPGAVILDQYNN
MMNPEAHYFGTGREIQRQLEDLNLFDNLRAVVAGAGTGGTISGISKYLKEQNDKIQIV
GADPFGSILAQPENLNKTDITDYKVEGIGYDFVPQVLDRKLIDVWYKTDDKPSFKYAR
QLISNEGVLVGGSSGSAFTAVVKYCEDHPELTEDDVIVAIFPDSIRSYLTKFVDDEWL
KKNNLWDDDVLARFDSSKLEASTTKYADVFGNATVKDLHLKPVVSVKETAKVTDVIKI
LKDNGFDQLPVLTEDGKLSGLVTLSELLRKLSINNSNNDNTIKGKYLDFKKLNNFNDV
SSYNENKSGKKKFIKFDENSKLSDLNRFFEKNSSAVITDGLKPIHIVTKMDLLSYLA"
gene <800546..>801823
/gene="PTI1"
/locus_tag="YGR156W"
/db_xref="GeneID:853060"
mRNA <800546..>801823
/gene="PTI1"
/locus_tag="YGR156W"
/product="cleavage polyadenylation factor subunit PTI1"
/transcript_id="NM_001181285.1"
/db_xref="GeneID:853060"
CDS 800546..801823
/gene="PTI1"
/locus_tag="YGR156W"
/experiment="EXISTENCE:curator inference:GO:0003723 RNA
binding [PMID:12819204]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:24390141|PMID:22932476|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005847 mRNA
cleavage and polyadenylation specificity factor complex
[PMID:12819204]"
/experiment="EXISTENCE:mutant phenotype:GO:0006397 mRNA
processing [PMID:12504017]"
/experiment="EXISTENCE:mutant phenotype:GO:0030847
termination of RNA polymerase II transcription,
exosome-dependent [PMID:12773397]"
/experiment="EXISTENCE:mutant phenotype:GO:0031126
sno(s)RNA 3'-end processing [PMID:12773397]"
/note="Essential component of CPF (cleavage and
polyadenylation factor); involved in 3' end formation of
snoRNA and mRNA; interacts directly with Pta1p;
relocalizes to the cytosol in response to hypoxia; similar
to mammalian Cleavage-Stimulation Factor CstF-64"
/codon_start=1
/product="cleavage polyadenylation factor subunit PTI1"
/protein_id="NP_011672.1"
/db_xref="GeneID:853060"
/db_xref="SGD:S000003388"
/translation="MTDPRRRTGRHFLTPENLSSTLQITNLPPEWNQDIITSVVAGSG
PVIDIKAKNDPRTGKLTGVLFDYLTSKDCKRAWEILNRIENFPVKIEQIIPPNYKDHL
RETANKNSQKQVLQLNRDSYPFEAGLELPFEMVTEVPIPRRPPPPQAANNTNSVSNNT
NIQFPDILSKASKHLPSFQDGSIIAPDKISQNLSKIPPLQLIEIISNLKILSNQENIQ
KSQLESFLDTNSDITISVTQALLEMGFIDYSVVTKVLKSQVGEAPSLLSSNNTSNSNT
PVSVIRNNTPLHVPSNEVSNNPNNMPLNVAMPMPMSTPPFIPLPLQQQPFGFAPPGPF
MPPAQGPSMGQPVLANQLGQVQQQNISSTEGPSNANKANDSGTINMAKLQLLPENQQD
MIKQVLTLTPAQIQSLPSDQQLMVENFRKEYII"
gene <802440..>805049
/gene="CHO2"
/locus_tag="YGR157W"
/gene_synonym="PEM1"
/db_xref="GeneID:853061"
mRNA <802440..>805049
/gene="CHO2"
/locus_tag="YGR157W"
/gene_synonym="PEM1"
/product="phosphatidylethanolamine N-methyltransferase"
/transcript_id="NM_001181286.1"
/db_xref="GeneID:853061"
CDS 802440..805049
/gene="CHO2"
/locus_tag="YGR157W"
/gene_synonym="PEM1"
/EC_number="2.1.1.17"
/experiment="EXISTENCE:direct assay:GO:0004608
phosphatidylethanolamine N-methyltransferase activity
[PMID:2445736|PMID:3066687]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006656
phosphatidylcholine biosynthetic process
[PMID:3066687|PMID:2445736]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0004608
phosphatidylethanolamine N-methyltransferase activity
[PMID:2445736]"
/experiment="EXISTENCE:genetic interaction:GO:0006656
phosphatidylcholine biosynthetic process [PMID:2445736]"
/experiment="EXISTENCE:mutant phenotype:GO:0004608
phosphatidylethanolamine N-methyltransferase activity
[PMID:3066687]"
/experiment="EXISTENCE:mutant phenotype:GO:0006656
phosphatidylcholine biosynthetic process
[PMID:3066687|PMID:23000174]"
/note="Phosphatidylethanolamine methyltransferase (PEMT);
catalyzes the first step in the conversion of
phosphatidylethanolamine to phosphatidylcholine during the
methylation pathway of phosphatidylcholine biosynthesis"
/codon_start=1
/product="phosphatidylethanolamine N-methyltransferase"
/protein_id="NP_011673.1"
/db_xref="GeneID:853061"
/db_xref="SGD:S000003389"
/translation="MSSCKTTLSEMVGSVTKDRGTINVEARTRSSNVTFKPPVTHDMV
RSLFDPTLKKSLLEKCIALAIISNFFICYWVFQRFGLQFTKYFFLVQYLFWRIAYNLG
IGLVLHYQSHYETLTNCAKTHAIFSKIPQNKDANSNFSTNSNSFSEKFWNFIRKFCQY
EIRSKMPKEYDLFAYPEEINVWLIFRQFVDLILMQDFVTYIIYVYLSIPYSWVQIFNW
RSLLGVILILFNIWVKLDAHRVVKDYAWYWGDFFFLEESELIFDGVFNISPHPMYSIG
YLGYYGLSLICNDYKVLLVSVFGHYSQFLFLKYVENPHIERTYGDGTDSDSQMNSRID
DLISKENYDYSRPLINMGLSFNNFNKLRFTDYFTIGTVAALMLGTIMNARFINLNYLF
ITVFVTKLVSWLFISTILYKQSQSKWFTRLFLENGYTQVYSYEQWQFIYNYYLVLTYT
LMIIHTGLQIWSNFSNINNSQLIFGLILVALQTWCDKETRLAISDFGWFYGDFFLSNY
ISTRKLTSQGIYRYLNHPEAVLGVVGVWGTVLMTNFAVTNIILAVLWTLTNFILVKFI
ETPHVNKIYGKTKRVSGVGKTLLGLKPLRQVSDIVNRIENIIIKSLVDESKNSNGGAE
LLPKNYQDNKEWNILIQEAMDSVATRLSPYCELKIENEQVETNFVLPTPVTLNWKMPI
ELYNGDDWIGLYKVIDTRADREKTRVGSGGHWSATSKDSYMNHGLRHKESVTEIKATE
KYVQGKVTFDTSLLYFENGIYEFRYHSGNSHKVLLISTPFEISLPVLNTTTPELFEKD
LTEFLTKVNVLKDGKFRPLGNKFFGMDSLKQLIKNSIGVELSSEYMRRVNGDAHVISH
RAWDIKQTLDSLA"
gene complement(<805269..>806021)
/gene="MTR3"
/locus_tag="YGR158C"
/db_xref="GeneID:853062"
mRNA complement(<805269..>806021)
/gene="MTR3"
/locus_tag="YGR158C"
/product="exosome non-catalytic core subunit MTR3"
/transcript_id="NM_001181287.3"
/db_xref="GeneID:853062"
CDS complement(805269..806021)
/gene="MTR3"
/locus_tag="YGR158C"
/experiment="EXISTENCE:direct assay:GO:0000176 nuclear
exosome (RNase complex) [PMID:19046973|PMID:10465791]"
/experiment="EXISTENCE:direct assay:GO:0000177 cytoplasmic
exosome (RNase complex) [PMID:10465791|PMID:19046973]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:8534909]"
/experiment="EXISTENCE:direct assay:GO:0071038
TRAMP-dependent tRNA surveillance pathway
[PMID:15828860|PMID:17643380|PMID:15935758]"
/experiment="EXISTENCE:mutant phenotype:GO:0000467
exonucleolytic trimming to generate mature 3'-end of 5.8S
rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S
rRNA, LSU-rRNA) [PMID:10508172|PMID:10465791]"
/experiment="EXISTENCE:mutant phenotype:GO:0034475 U4
snRNA 3'-end processing [PMID:10611222]"
/experiment="EXISTENCE:mutant phenotype:GO:0071028 nuclear
mRNA surveillance [PMID:11030620]"
/experiment="EXISTENCE:mutant phenotype:GO:0071035 nuclear
polyadenylation-dependent rRNA catabolic process
[PMID:18007593|PMID:10465791]"
/experiment="EXISTENCE:mutant phenotype:GO:0071039 nuclear
polyadenylation-dependent CUT catabolic process
[PMID:18591258]"
/experiment="EXISTENCE:mutant phenotype:GO:0071051
poly(A)-dependent snoRNA 3'-end processing
[PMID:10611222]"
/note="Exosome non-catalytic core component; involved in
3'-5' RNA processing and degradation in both the nucleus
and the cytoplasm; has similarity to E. coli RNase PH and
to human hMtr3p (EXOSC6)"
/codon_start=1
/product="exosome non-catalytic core subunit MTR3"
/protein_id="NP_011674.3"
/db_xref="GeneID:853062"
/db_xref="SGD:S000003390"
/translation="MNVQDRRRLLGPAAAKPMAFSNTTTHVPEKKSTDLTPKGNESEQ
ELSLHTGFIENCNGSALVEARSLGHQTSLITAVYGPRSIRGSFTSQGTISIQLKNGLL
EKYNTNELKEVSSFLMGIFNSVVNLSRYPKSGIDIFVYLTYDKDLTNNPQDDDSQSKM
MSSQISSLIPHCITSITLALADAGIELVDMAGAGEANGTVVSFIKNGEEIVGFWKDDG
DDEDLLECLDRCKEQYNRYRDLMISCLMNQET"
gene complement(<806412..>807656)
/gene="NSR1"
/locus_tag="YGR159C"
/gene_synonym="SHE5"
/db_xref="GeneID:853064"
mRNA complement(<806412..>807656)
/gene="NSR1"
/locus_tag="YGR159C"
/gene_synonym="SHE5"
/product="Nsr1p"
/transcript_id="NM_001181288.1"
/db_xref="GeneID:853064"
CDS complement(806412..807656)
/gene="NSR1"
/locus_tag="YGR159C"
/gene_synonym="SHE5"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:19861225]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:1706724|PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:1706724]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0008139 nuclear
localization sequence binding [PMID:1706724]"
/experiment="EXISTENCE:direct assay:GO:0043047
single-stranded telomeric DNA binding [PMID:7800479]"
/experiment="EXISTENCE:direct assay:GO:0051880
G-quadruplex DNA binding [PMID:33106247]"
/experiment="EXISTENCE:mutant phenotype:GO:0006310 DNA
recombination [PMID:33106247]"
/experiment="EXISTENCE:mutant phenotype:GO:0051276
chromosome organization [PMID:33106247]"
/experiment="EXISTENCE:mutant phenotype:GO:0051880
G-quadruplex DNA binding [PMID:33106247]"
/note="Nucleolin, nucleolar protein that binds nuclear
localization sequences; required for pre-rRNA processing
and ribosome biogenesis; binds to single stranded
telomeric DNA and mRNA; binds to G-quadruplex DNA;
methylated by Hmt1p; interaction with Top1p and nucleolar
localization are negatively regulated by lysine
polyphosphorylation"
/codon_start=1
/product="Nsr1p"
/protein_id="NP_011675.1"
/db_xref="GeneID:853064"
/db_xref="SGD:S000003391"
/translation="MAKTTKVKGNKKEVKASKQAKEEKAKAVSSSSSESSSSSSSSSE
SESESESESESSSSSSSSDSESSSSSSSDSESEAETKKEESKDSSSSSSDSSSDEEEE
EEKEETKKEESKESSSSDSSSSSSSDSESEKEESNDKKRKSEDAEEEEDEESSNKKQK
NEETEEPATIFVGRLSWSIDDEWLKKEFEHIGGVIGARVIYERGTDRSRGYGYVDFEN
KSYAEKAIQEMQGKEIDGRPINCDMSTSKPAGNNDRAKKFGDTPSEPSDTLFLGNLSF
NADRDAIFELFAKHGEVVSVRIPTHPETEQPKGFGYVQFSNMEDAKKALDALQGEYID
NRPVRLDFSSPRPNNDGGRGGSRGFGGRGGGRGGNRGFGGRGGARGGRGGFRPSGSGA
NTAPLGRSRNTASFAGSKKTFD"
gene complement(<808629..>809420)
/gene="RTS3"
/locus_tag="YGR161C"
/db_xref="GeneID:853065"
mRNA complement(<808629..>809420)
/gene="RTS3"
/locus_tag="YGR161C"
/product="Rts3p"
/transcript_id="NM_001181290.3"
/db_xref="GeneID:853065"
CDS complement(808629..809420)
/gene="RTS3"
/locus_tag="YGR161C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="Putative component of the protein phosphatase type
2A complex"
/codon_start=1
/product="Rts3p"
/protein_id="NP_011677.3"
/db_xref="GeneID:853065"
/db_xref="SGD:S000003393"
/translation="MIATSRAVNMNKESKHKKAVAKPCRERQTSVTRAMRPAVARDPR
RLSTSSSPSSSPMSAQRRLSREEIINEMEKEQDAIVVRLLREIETLKEENSRLKNQLH
HPVPARRSSPFFEGESAILDDDDCNYGYTLDTPKLKLTDGASRHTVLPLTPKDSMTHI
SHSARRSSRNASISNGTSISDTIFPIETKIHSAPTTNRNLPSADLPHHTLLPRSLSGI
SSSDLTESGALLHDRRRRSSNYSLDGSNSLKADLMAKRFQTGSLK"
gene <810227..>810505
/locus_tag="YGR161W-C"
/db_xref="GeneID:3077355"
mRNA <810227..>810505
/locus_tag="YGR161W-C"
/product="uncharacterized protein"
/transcript_id="NM_001184675.3"
/db_xref="GeneID:3077355"
CDS 810227..810505
/locus_tag="YGR161W-C"
/note="hypothetical protein; identified by sequence
comparison with hemiascomycetous yeast species"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001018033.3"
/db_xref="GeneID:3077355"
/db_xref="SGD:S000029726"
/translation="MSGYFNHLSSNAHFANIQADQGFIGDATGTSSDHGSSGMVDFAL
QLGELSLEEKILKEFTLFQSKNMDLLQETATACPSTNPSLRQSRIQGW"
mobile_element 811447..817397
/note="YGRWTy2-2; Ty2 element, LTR retrotransposon of the
Copia (Pseudoviridae) group; contains co-transcribed genes
TYA Gag and TYB Pol, encoding proteins involved in
structure and function of virus-like particles, flanked by
two direct repeats"
/mobile_element_type="retrotransposon:YGRWTy2-2"
/db_xref="SGD:S000006983"
repeat_region 811447..811778
/note="Ty2 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006974"
gene <811738..>817051
/locus_tag="YGR161W-B"
/db_xref="GeneID:853067"
mRNA <811738..>817051
/locus_tag="YGR161W-B"
/product="gag-pol fusion protein"
/transcript_id="NM_001184402.2"
/db_xref="GeneID:853067"
CDS join(811738..813030,813032..817051)
/locus_tag="YGR161W-B"
/EC_number="2.7.7.7"
/EC_number="2.7.7.49"
/EC_number="3.1.26.4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9448009]"
/ribosomal_slippage
/note="Retrotransposon TYA Gag and TYB Pol genes;
transcribed/translated as one unit; polyprotein is
processed to make a nucleocapsid-like protein (Gag),
reverse transcriptase (RT), protease (PR), and integrase
(IN); similar to retroviral genes"
/codon_start=1
/product="gag-pol fusion protein"
/protein_id="NP_058163.1"
/db_xref="GeneID:853067"
/db_xref="SGD:S000007370"
/translation="MESQQLHQNPHSLHGSAYASVTSKEVPSNQDPLAVSASNLPEFD
RDSTKVNSQQETTPGTSAVPENHHHVSPQPASVPPPQNGQYQQHGMMTPNKAMASNWA
HYQQPSMMTCSHYQTSPAYYQPDPHYPLPQYIPPLSTSSPDPIDSQNQHSEVPQAETK
VRNNVLPPHTLTSEENFSTWVKFYIRFLKNSNLGDIIPNDQGEIKSQMTYEEHAYIYN
TFQAFAPFHLLPTWVKQILEINYADILTVLCKSVSKMQTNNQELKDWIALANLEYDGS
TSADTFEITVSTIIQRLKENNINVSDRLACQLILKGLSGDFKYLRNQYRTKTNMKLSQ
LFAEIQLIYDENKIMNLNKPSQYKQHSEYKNVSRTSPNTTNTKVTTRNYHRTNSSKPR
AAKAHNIATSSKFSRVNNDHINESTVSSQYLSDDNELSLGQQQKESKPTHTIDSNDEL
PDHLLIDSGASQTLVRSAHYLHHATPNSEINIVDAQKQDIPINAIGNLHFNFQNGTKT
SIKALHTPNIAYDLLSLSELANQNITACFTRNTLERSDGTVLAPIVKHGDFYWLSKKY
LIPSHISKLTINNVNKSKSVNKYPYPLIHRMLGHANFRSIQKSLKKNAVTYLKESDIE
WSNASTYQCPDCLIGKSTKHRHVKGSRLKYQESYEPFQYLHTDIFGPVHHLPKSAPSY
FISFTDEKTRFQWVYPLHDRREESILNVFTSILAFIKNQFNARVLVIQMDRGSEYTNK
TLHKFFTNRGITACYTTTADSRAHGVAERLNRTLLNDCRTLLHCSGLPNHLWFSAVEF
STIIRNSLVSPKNDKSARQHAGLAGLDITTILPFGQPVIVNNHNPDSKIHPRGIPGYA
LHPSRNSYGYIIYLPSLKKTVDTTNYVILQDKQSKLDQFNYDTLTFDDDLNRLTAHNQ
SFIEQNETEQSYDQNTESDHDYQSEIEINSDPLVNDFSSQSINPLQLDKEPVQKVRAP
KEVDADISEYNILPSTIRSRTPHIINKESTEMGGTVESDTTSPRHSSTFTARNQNRPG
STNEMIDLTSQDRVNYGLENIKTTRLGGTEEPYIQRNSDTNIKYRTTNSTPSIDDRSS
NSESTTPIISIETKAVCDNTPSIDTDPPEYRSSDHATPNIMPDKSSKNVTADSILDDL
PLPDLTHKSPTDTSDVSKDIPHIHSRQTNSSLGGMDDSNVLTTTKSKKRSLEDNETEI
EVSRDTWNNKNMRSLEPPRSKKRINLIAAIKGVKSIKPVRTTLRYDEAITYNKDNKEK
DRYVEAYHKEISQLLKMNTWDTNKYYDRNDIDPKKVINSMFIFNKKRDGTHKARFVAR
GDIQHPDTYDSDMQSNTVHHYALMTSLSIALDNDYYITQLDISSAYLYADIKEELYIR
PPPHLGLNDKLLRLRKSLYGLKQSGANWYETIKSYLINCCDMQEVRGWSCVFKNSQVT
ICLFVDDMILFSKDLNANKKIITTLKKQYDTKIINLGESDNEIQYDILGLEIKYQRSK
YMKLGMEKSLTEKLPKLNVPLNPKGKKLRAPGQPGHYIDQDELEIDEDEYKEKVHEMQ
KLIGLASYVGYKFRFDLLYYINTLAQHILFPSRQVLDMTYELIQFMWDTRDKQLIWHK
NKPTKPDNKLVAISDASYGNQPYYKSQIGNIFLLNGKVIGGKSTKASLTCTSTTEAEI
HAVSEAIPLLNNLSHLVQELNKKPIIKGLLTDSRSTISIIKSTNEEKFRNRFFGTKAM
RLRDEVSGNNLYVYYIETKKNIADVMTKPLPIKTFKLLTNKWIH"
gene <811738..>813054
/locus_tag="YGR161W-A"
/db_xref="GeneID:853066"
mRNA <811738..>813054
/locus_tag="YGR161W-A"
/product="gag protein"
/transcript_id="NM_001184401.1"
/db_xref="GeneID:853066"
CDS 811738..813054
/locus_tag="YGR161W-A"
/note="Retrotransposon TYA Gag gene co-transcribed with
TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is
a nucleocapsid protein that is the structural constituent
of virus-like particles (VLPs); similar to retroviral Gag"
/codon_start=1
/product="gag protein"
/protein_id="NP_058164.1"
/db_xref="GeneID:853066"
/db_xref="SGD:S000007369"
/translation="MESQQLHQNPHSLHGSAYASVTSKEVPSNQDPLAVSASNLPEFD
RDSTKVNSQQETTPGTSAVPENHHHVSPQPASVPPPQNGQYQQHGMMTPNKAMASNWA
HYQQPSMMTCSHYQTSPAYYQPDPHYPLPQYIPPLSTSSPDPIDSQNQHSEVPQAETK
VRNNVLPPHTLTSEENFSTWVKFYIRFLKNSNLGDIIPNDQGEIKSQMTYEEHAYIYN
TFQAFAPFHLLPTWVKQILEINYADILTVLCKSVSKMQTNNQELKDWIALANLEYDGS
TSADTFEITVSTIIQRLKENNINVSDRLACQLILKGLSGDFKYLRNQYRTKTNMKLSQ
LFAEIQLIYDENKIMNLNKPSQYKQHSEYKNVSRTSPNTTNTKVTTRNYHRTNSSKPR
AAKAHNIATSSKFSRVNNDHINESTVSSQYLSDDNELSLRPATERI"
repeat_region 817074..817397
/note="Ty2 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006975"
mobile_element complement(817393..823306)
/note="YGRCTy1-3; Ty1 element, LTR retrotransposon of the
Copia (Pseudoviridae) group; contains co-transcribed genes
TYA Gag and TYB Pol, encoding proteins involved in
structure and function of virus-like particles, flanked by
two direct repeats"
/mobile_element_type="retrotransposon:YGRCTy1-3"
/db_xref="SGD:S000006963"
repeat_region complement(817393..817724)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006959"
gene complement(<817747..>823015)
/locus_tag="YGR161C-D"
/db_xref="GeneID:853068"
mRNA complement(<817747..>823015)
/locus_tag="YGR161C-D"
/product="gag-pol fusion protein"
/transcript_id="NM_001184400.4"
/db_xref="GeneID:853068"
CDS complement(join(817747..821709,821711..823015))
/locus_tag="YGR161C-D"
/EC_number="2.7.7.7"
/EC_number="2.7.7.49"
/EC_number="3.1.26.4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9448009]"
/ribosomal_slippage
/note="Retrotransposon TYA Gag and TYB Pol genes;
transcribed/translated as one unit; polyprotein is
processed to make a nucleocapsid-like protein (Gag),
reverse transcriptase (RT), protease (PR), and integrase
(IN); similar to retroviral genes"
/codon_start=1
/product="gag-pol fusion protein"
/protein_id="NP_058165.3"
/db_xref="GeneID:853068"
/db_xref="SGD:S000007368"
/translation="MESQQLSNYPHISHGSACASVTSKEVHTNQDPLDVSASKIQEYD
KASTKANSQQTTTPASSAVPENPHHASPQPASVPPPQNGPYPQQCMMTQNQANPSGWS
FYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVRQITDDELT
FLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQ
YNGSTPADAFETKVTNIIDRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNM
TVAELFLDIHAIYEEQQGSRNSKPNYRRNPSDEKNDSRSYTNTTKPKVIARNPQKTNN
SKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLGQKLTESTVNHTNHS
DDELPGHLLLDSGASRTLIRSAHHIHSASSNPDINVVDAQKRNIPINAIGDLQFHFQD
NTKTSIKVLHTPNIAYDLLSLNELAAVDITACFTKNVLERSDGTVLAPIVKYGDFYWV
SKKYLLPSNISVPTINNVHTSESTRKYPYPFIHRMLAHANAQTIRYSLKNNTITYFNE
SDVDWSSAIDYQCPDCLIGKSTKHRHIKGSRLKYQNSYEPFQYLHTDIFGPVHNLPKS
APSYFISFTDETTKFRWVYPLHDRREDSILDVFTTILAFIKNQFQASVLVIQMDRGSE
YTNRTLHKFLEKNGITPCYTTTADSRAHGVAERLNRTLLDDCRTQLQCSGLPNHLWFS
AIEFSTIVRNSLASPKSKKSARQHAGLAGLDISTLLPFGQPVIVNDHNPNSKIHPRGI
PGYALHPSRNSYGYIIYLPSLKKTVDTTNYVILQGKESRLDQFNYDALTFDEDLNRLT
ASYHSFIASNEIQESNDLNIESDHDFQSDIELHPEQPRNVLSKAVSPTDSTPPSTHTE
DSKRVSKTNIRAPREVDPNISESNILPSKKRSSTPQISNIESTGSGGMHKLNVPLLAP
MSQSNTHESSYASKSKDFRHSDSYSDNETNHTNVPISSTGGTNNKTVPQTSEQETEKR
IIHRSPSIDTSSSESNSLHHVVPIKTSDTCPKENTEESIIADLPLPDLPPEPPTELSD
SFKELPPINSRQTNSSLGGIGDSNAYTTINSKKRSLEDNETEIKVSRDTWNTKNMRSL
EPPRSKKRIHLIAAVKAVKSIKPIRTTLRYDEAITYNKDIKEKEKYIEAYHKEVNQLL
KMNTWDTDKYYDRKEIDPKRVINSMFIFNRKRDGTHKARFVARGDIQHPDTYDSGMQS
NTVHHYALMTSLSLALDNNYYITQLDISSAYLYADIKEELYIRPPPHLGMNDKLIRLK
KSLYGLKQSGANWYETIKSYLIKQCGMEEVRGWSCVFKNSQVTICLFVDDMILFSKDL
NSNKRIIAKLKMQYDTKIINLGESDDEIQYDILGLEIKYQRGKYMKLGMENSLTEKIP
KLNVPLNPNGRKLGAPGQPGLYINQQELELEEDDYKMKVHEMQKLIGLASYVGYKFRF
DLLYYINTLAQHILFPSKQVLDMTYELIQFIWNTRDKQLIWHKSKPVKPTNKLVVISD
ASYGNQPYYKSQIGNIYLLNGKVIGGKSTKASLTCTSTTEAEIHAISESVPLLNNLSY
LIQELDKKPITKGLLTDSKSTISIIISNNEEKFRNRFFGTKAMRLRDEVSGNHLHVCY
IETKKNIADVMTKPLPIKTFKLLTNKWIH"
gene complement(<821693..>823015)
/locus_tag="YGR161C-C"
/db_xref="GeneID:853069"
mRNA complement(<821693..>823015)
/locus_tag="YGR161C-C"
/product="gag protein"
/transcript_id="NM_001184399.1"
/db_xref="GeneID:853069"
CDS complement(821693..823015)
/locus_tag="YGR161C-C"
/note="Retrotransposon TYA Gag gene co-transcribed with
TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is
a nucleocapsid protein that is the structural constituent
of virus-like particles (VLPs); similar to retroviral Gag"
/codon_start=1
/product="gag protein"
/protein_id="NP_058166.1"
/db_xref="GeneID:853069"
/db_xref="SGD:S000007367"
/translation="MESQQLSNYPHISHGSACASVTSKEVHTNQDPLDVSASKIQEYD
KASTKANSQQTTTPASSAVPENPHHASPQPASVPPPQNGPYPQQCMMTQNQANPSGWS
FYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVRQITDDELT
FLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQ
YNGSTPADAFETKVTNIIDRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNM
TVAELFLDIHAIYEEQQGSRNSKPNYRRNPSDEKNDSRSYTNTTKPKVIARNPQKTNN
SKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLRPETY"
repeat_region complement(822975..823306)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006960"
gene 823482..823555
/locus_tag="YNCG0037W"
/db_xref="GeneID:853070"
tRNA 823482..823555
/locus_tag="YNCG0037W"
/product="tRNA-Val"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Valine tRNA (tRNA-Val), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:853070"
/db_xref="SGD:S000006756"
gene <824059..>826917
/gene="TIF4631"
/locus_tag="YGR162W"
/db_xref="GeneID:853071"
mRNA <824059..>826917
/gene="TIF4631"
/locus_tag="YGR162W"
/product="translation initiation factor eIF4G"
/transcript_id="NM_001181291.3"
/db_xref="GeneID:853071"
CDS 824059..826917
/gene="TIF4631"
/locus_tag="YGR162W"
/experiment="EXISTENCE:direct assay:GO:0000932 P-body
[PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0001671 ATPase
activator activity
[PMID:18606994|PMID:24080224|PMID:21062831]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:12810920]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:21925384]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule
[PMID:17908917|PMID:18981231|PMID:26777405]"
/experiment="EXISTENCE:direct assay:GO:1901195 positive
regulation of formation of translation preinitiation
complex [PMID:30281017]"
/experiment="EXISTENCE:mutant phenotype:GO:0006413
translational initiation [PMID:12810920]"
/experiment="EXISTENCE:mutant phenotype:GO:0016281
eukaryotic translation initiation factor 4F complex
[PMID:8336723]"
/experiment="EXISTENCE:mutant phenotype:GO:0034063 stress
granule assembly [PMID:18981231]"
/experiment="EXISTENCE:mutant phenotype:GO:0042149
cellular response to glucose starvation [PMID:18981231]"
/experiment="EXISTENCE:mutant phenotype:GO:0042273
ribosomal large subunit biogenesis [PMID:19806183]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:21925384]"
/note="Translation initiation factor eIF4G and scaffold
protein; subunit of the mRNA cap-binding protein complex
(eIF4F) with eIF4E (Cdc33p) and eIF4A (Tif1p/Tif2p);
stimulates the ATPase and helicase activities of eIF4A
synergistically with eIF4G; required with eIF4E for
Ded1p-mediated stimulation of mRNA recruitment during 48S
preinitiation complex (PIC) assembly; association with
Pab1p mediates poly(A) tail-stimulated translation; role
in assembly of PET region in biogenesis of 60S ribosomal
subunit"
/codon_start=1
/product="translation initiation factor eIF4G"
/protein_id="NP_011678.3"
/db_xref="GeneID:853071"
/db_xref="SGD:S000003394"
/translation="MTDETAHPTQSASKQESAALKQTGDDQQESQQQRGYTNYNNGSN
YTQKKPYNSNRPHQQRGGKFGPNRYNNRGNYNGGGSFRGGHMGANSSNVPWTGYYNNY
PVYYQPQQMAAAGSAPANPIPVEEKSPVPTKIEITTKSGEHLDLKEQHKAKLQSQERS
TVSPQPESKLKETSDSTSTSTPTPTPSTNDSKASSEENISEAEKTRRNFIEQVKLRKA
ALEKKRKEQLEGSSGNNNIPMKTTPENVEEKGSDKPEVTEKTKPAEEKSAEPEVKQET
PAEEGEQGEKGQIKEESTPKVLTFAERLKLKKQQKEREEKTEGKENKEVPVQEETKSA
IESAPVPPSEQVKEETEVAETEQSNIDESATTPAIPTKSDEAEAEVEAEAGDAGTKIG
LEAEIETTTDETDDGTNTVSHILNVLKDATPIEDVFSFNYPEGIEGPDIKYKKEHVKY
TYGPTFLLQFKDKLNVKADAEWVQSTASKIVIPPGMGRGNRSRDSGRFGNNSSRGHDF
RNTSVRNMDDRANSRTSSKRRSKRMNDDRRSNRSYTSRRDRERGSYRNEEKREDDKPK
EEVAPLVPSANRWVPKFKSKKTEKKLAPDGKTELLDKDEVERKMKSLLNKLTLEMFDA
ISSEILAIANISVWETNGETLKAVIEQIFLKACDEPHWSSMYAQLCGKVVKELNPDIT
DETNEGKTGPKLVLHYLVARCHAEFDKGWTDKLPTNEDGTPLEPEMMSEEYYAAASAK
RRGLGLVRFIGFLYRLNLLTGKMMFECFRRLMKDLTDSPSEETLESVVELLNTVGEQF
ETDSFRTGQATLEGSQLLDSLFGILDNIIQTAKISSRIKFKLIDIKELRHDKNWNSDK
KDNGPKTIQQIHEEEERQRQLKNNSRSNSRRTNNSSNRHSFRRDAPPASKDSFITTRT
YSQRNSQRAPPPKEEPAAPTSTATNMFSALMGESDDEE"
gene <827552..>828577
/gene="GTR2"
/locus_tag="YGR163W"
/db_xref="GeneID:853072"
mRNA <827552..>828577
/gene="GTR2"
/locus_tag="YGR163W"
/product="Gtr2p"
/transcript_id="NM_001181292.3"
/db_xref="GeneID:853072"
CDS 827552..828577
/gene="GTR2"
/locus_tag="YGR163W"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane
[PMID:23836928|PMID:15989961|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:18258182]"
/experiment="EXISTENCE:direct assay:GO:0003924 GTPase
activity [PMID:26387955]"
/experiment="EXISTENCE:direct assay:GO:0005525 GTP binding
[PMID:16143306]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10388807|PMID:11073942]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:10388807|PMID:11073942]"
/experiment="EXISTENCE:direct assay:GO:0031902 late
endosome membrane [PMID:16732272]"
/experiment="EXISTENCE:direct assay:GO:0045121 membrane
raft [PMID:23836928]"
/experiment="EXISTENCE:direct assay:GO:0045944 positive
regulation of transcription by RNA polymerase II
[PMID:18258182]"
/experiment="EXISTENCE:direct assay:GO:0071986 Ragulator
complex [PMID:16732272]"
/experiment="EXISTENCE:genetic interaction:GO:0006995
cellular response to nitrogen starvation [PMID:25046117]"
/experiment="EXISTENCE:genetic interaction:GO:0010507
negative regulation of autophagy [PMID:25046117]"
/experiment="EXISTENCE:genetic interaction:GO:1904263
positive regulation of TORC1 signaling [PMID:19748353]"
/experiment="EXISTENCE:mutant phenotype:GO:0016237
microautophagy [PMID:15989961]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress [PMID:35158208]"
/experiment="EXISTENCE:mutant phenotype:GO:0071230
cellular response to amino acid stimulus [PMID:26387955]"
/experiment="EXISTENCE:mutant phenotype:GO:1903778 protein
localization to vacuolar membrane [PMID:25046117]"
/experiment="EXISTENCE:mutant phenotype:GO:1904263
positive regulation of TORC1 signaling
[PMID:26387955|PMID:19748353]"
/experiment="EXISTENCE:physical interaction:GO:0071986
Ragulator complex [PMID:15989961]"
/experiment="EXISTENCE:physical interaction:GO:1990131
Gtr1-Gtr2 GTPase complex [PMID:10388807|PMID:16143306]"
/note="Subunit of a TORC1-stimulating GTPase and the
EGO/GSE complex; subunit of Gtr1-Gtr2, a GTPase that
activates TORC1 in response to amino acid stimulation;
negatively regulates the GTPase cycle of Gtr1p, a Ran/TC4
homolog; subunit of EGO, a vacuolar membrane complex that
regulates exit from rapamycin-induced growth arrest and
esorting of Gap1p; activates transcription when chromatin
bound; role in the oxidative stress response; activated by
the Lst4p-Lst7p GAP complex; homolog of human RagC"
/codon_start=1
/product="Gtr2p"
/protein_id="NP_011679.3"
/db_xref="GeneID:853072"
/db_xref="SGD:S000003395"
/translation="MSLEATDSKAMVLLMGVRRCGKSSICKVVFHNMQPLDTLYLEST
SNPSLEHFSTLIDLAVMELPGQLNYFEPSYDSERLFKSVGALVYVIDSQDEYINAITN
LAMIIEYAYKVNPSINIEVLIHKVDGLSEDFKVDAQRDIMQRTGEELLELGLDGVQVS
FYLTSIFDHSIYEAFSRIVQKLIPELSFLENMLDNLIQHSKIEKAFLFDVNSKIYVST
DSNPVDIQMYEVCSEFIDVTIDLFDLYKAPVLRNSQKSSDKDNVINPRNELQNVSQLA
NGVIIYLRQMIRGLALVAIIRPNGTDMESCLTVADYNIDIFKKGLEDIWANARASQAK
NSIEDDV"
gene <828625..>828960
/locus_tag="YGR164W"
/db_xref="GeneID:853073"
mRNA <828625..>828960
/locus_tag="YGR164W"
/product="uncharacterized protein"
/transcript_id="NM_001348843.1"
/db_xref="GeneID:853073"
CDS 828625..828960
/locus_tag="YGR164W"
/note="hypothetical protein; conserved across S.
cerevisiae strains"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335783.1"
/db_xref="GeneID:853073"
/db_xref="SGD:S000003396"
/translation="MIVEPMTKPRKPTVQRCQSFFTNGANHFYCKKSTHDGGRTHNLL
IRSQTRCHYATRATVCWKFSIINKYIPTLANITITTLRKLYKRFIDRESLFLIFFRKD
EHIVQNIIN"
gene complement(828723..828794)
/locus_tag="YNCG0038C"
/db_xref="GeneID:853074"
tRNA complement(828723..828794)
/locus_tag="YNCG0038C"
/product="tRNA-Arg"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE
analysis; one of 11 nuclear tRNA genes containing the
tDNA-anticodon UCU (converted to mcm5-UCU in the mature
tRNA), decodes AGA codons into arginine, one of 19 nuclear
tRNAs for arginine"
/db_xref="GeneID:853074"
/db_xref="SGD:S000006712"
gene <829116..>830153
/gene="MRPS35"
/locus_tag="YGR165W"
/gene_synonym="mS45"
/db_xref="GeneID:853075"
mRNA <829116..>830153
/gene="MRPS35"
/locus_tag="YGR165W"
/gene_synonym="mS45"
/product="mitochondrial 37S ribosomal protein mS45 MRPS35"
/transcript_id="NM_001181294.1"
/db_xref="GeneID:853075"
CDS 829116..830153
/gene="MRPS35"
/locus_tag="YGR165W"
/gene_synonym="mS45"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:12392552]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:12392552]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005763
mitochondrial small ribosomal subunit [PMID:12392552]"
/note="Mitochondrial ribosomal protein of the small
subunit; null mutant does not grow on glycerol, is
sensitive to 2,4-dichlorophenol, and accumulates large
lipid droplets"
/codon_start=1
/product="mitochondrial 37S ribosomal protein mS45 MRPS35"
/protein_id="NP_011681.1"
/db_xref="GeneID:853075"
/db_xref="SGD:S000003397"
/translation="MSYGLTGTSSKLRGTSSIFSWTQVRHFSRRRIAYPFYPFKKLGR
QHPKKHDTNLKTAMRQFLGPKNYKGEYVMNKYFTVPTNHVPNYIKPDLERGQSLEHPV
TKKPLQLRYDGTLGPPPVENKRLQNIFKDRLLQPFPSNPHCKTNYVLSPQLKQSIFEE
ITVEGLSAQQVSQKYGLKIPRVEAIVKLVSVENSWNRRNRVSSDLKTMDETLYRMFPV
FDSDASFKRENLSEIPVPQKTLASRFLTIAESEPFGPVDAAHVLELEPAVETLRNLST
VGEHSSGHQQSTNKNTKVIYGELVEGERSQYKFTNAKVGKVGYRYGSGNRDNKKDRRI
GFNKLGQMVYI"
gene <830515..>832197
/gene="TRS65"
/locus_tag="YGR166W"
/gene_synonym="KRE11"
/db_xref="GeneID:853076"
mRNA <830515..>832197
/gene="TRS65"
/locus_tag="YGR166W"
/gene_synonym="KRE11"
/product="Trs65p"
/transcript_id="NM_001181295.1"
/db_xref="GeneID:853076"
CDS 830515..832197
/gene="TRS65"
/locus_tag="YGR166W"
/gene_synonym="KRE11"
/experiment="EXISTENCE:direct assay:GO:0005802 trans-Golgi
network [PMID:17475775]"
/experiment="EXISTENCE:direct assay:GO:1990071 TRAPPII
protein complex [PMID:11239471]"
/experiment="EXISTENCE:genetic interaction:GO:0005085
guanyl-nucleotide exchange factor activity
[PMID:17475775]"
/experiment="EXISTENCE:genetic interaction:GO:0006891
intra-Golgi vesicle-mediated transport [PMID:17475775]"
/experiment="EXISTENCE:genetic interaction:GO:0065003
protein-containing complex assembly [PMID:17475775]"
/experiment="EXISTENCE:physical interaction:GO:0006891
intra-Golgi vesicle-mediated transport [PMID:17475775]"
/note="Component of transport protein particle (TRAPP)
complex II; TRAPPII is a multimeric guanine
nucleotide-exchange factor for the GTPase Ypt1p,
regulating intra-Golgi and endosome-Golgi traffic; role in
cell wall beta-glucan biosynthesis and the stress
response"
/codon_start=1
/product="Trs65p"
/protein_id="NP_011682.1"
/db_xref="GeneID:853076"
/db_xref="SGD:S000003398"
/translation="MECFVPLRCDLDGSNIEQLRQSHLSRKFIIFDEQLNLWLWFQGN
SQENKRFVLQNMIILINEAQVTRTSTIDDYFTQVENNENLWRLKNDCCSKILFKSNVV
MNNGYNNQIKFVFEYKSVDANFNNQDSLQDPQAKYTLDKYSSEEILPSFEPVYSWSSA
ATKSSKNTNNHLEKNNRATHRVSSKNSEVHEADVSRNPNTFTLKLQYPIFSLLNMRLR
NISLKSEHCILSSLDFQTSKASEQLTKKFIYPQEHNSFLKLNFQEISYKLIDGTSQIE
LDPICPLKVPLTAFSYDSISATFKLVLLPKSTQPHRVKITLAYELELHPNLKLPVRTS
WETEVTLKRSMPISSTSSQYSSNNNNTNHSASFNGAANNVNSGGLANLRLGGVSSSRF
SLGAASTTSLVNSKLSNVKFKFINSNIKVIKGEKFTMRLQIINSSSSPLDLVVYYNNT
INPIPSANNVRNSNGINNCGMNNGTIPNSPLTLENQYQLHNKYRKIAEGIILLSNDYK
IPVVPPRETYFADLRFIGIMSGYYGTLSGLKVLDLNTNELIEVGNGASVLIQ"
gene <832456..>833157
/gene="CLC1"
/locus_tag="YGR167W"
/gene_synonym="SCD4"
/db_xref="GeneID:853077"
mRNA <832456..>833157
/gene="CLC1"
/locus_tag="YGR167W"
/gene_synonym="SCD4"
/product="clathrin light chain CLC1"
/transcript_id="NM_001181296.3"
/db_xref="GeneID:853077"
CDS 832456..833157
/gene="CLC1"
/locus_tag="YGR167W"
/gene_synonym="SCD4"
/experiment="EXISTENCE:direct assay:GO:0005802 trans-Golgi
network [PMID:27062026]"
/experiment="EXISTENCE:direct assay:GO:0006897 endocytosis
[PMID:16643280]"
/experiment="EXISTENCE:mutant phenotype:GO:0006897
endocytosis [PMID:16643280]"
/experiment="EXISTENCE:mutant phenotype:GO:0045807
positive regulation of endocytosis [PMID:21849475]"
/experiment="EXISTENCE:mutant phenotype:GO:0065003
protein-containing complex assembly [PMID:27062026]"
/experiment="EXISTENCE:physical interaction:GO:0006897
endocytosis [PMID:16643280]"
/note="Clathrin light chain; subunit of the major coat
protein involved in intracellular protein transport and
endocytosis; regulates endocytic progression; thought to
regulate clathrin function; the clathrin triskelion is a
trimeric molecule composed of three heavy chains that
radiate from a vertex and three light chains which bind
noncovalently near the vertex of the triskelion"
/codon_start=1
/product="clathrin light chain CLC1"
/protein_id="NP_011683.3"
/db_xref="GeneID:853077"
/db_xref="SGD:S000003399"
/translation="MSEKFPPLEDQNIDFTPNDKKDDDTDFLKREAEILGDEFKTEQD
DILETEASPAKDDDEIRDFEEQFPDINSANGAVSSDQNGSATVSSGNDNGEADDDFST
FEGANQSTESVKEDRSEVVDQWKQRRAVEIHEKDLKDEELKKELQDEAIKHIDDFYDS
YNKKKEQQLEDAAKEAEAFLKKRDEFFGQDNTTWDRALQLINQDDADIIGGRDRSKLK
EILLRLKGNAKAPGA"
gene complement(<833352..>834482)
/gene="PEX35"
/locus_tag="YGR168C"
/db_xref="GeneID:853078"
mRNA complement(<833352..>834482)
/gene="PEX35"
/locus_tag="YGR168C"
/product="Pex35p"
/transcript_id="NM_001181297.1"
/db_xref="GeneID:853078"
CDS complement(833352..834482)
/gene="PEX35"
/locus_tag="YGR168C"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005778 peroxisomal
membrane [PMID:28049721]"
/experiment="EXISTENCE:mutant phenotype:GO:1900063
regulation of peroxisome organization [PMID:28049721]"
/note="Regulator of peroxisome abundance; peroxisomal
membrane protein, remote homolog to several
curvature-generating human proteins; functionally
interacts with vesicle-budding-inducing ADP-ribosylation
factor Arf1p"
/codon_start=1
/product="Pex35p"
/protein_id="NP_011684.1"
/db_xref="GeneID:853078"
/db_xref="SGD:S000003400"
/translation="MKHNRPNGTGKAVSGFKQIIRRLLLLLNKKRRKQLVIILKRITQ
VYGINLVFYVKKWKLKKLQGENIQINDIMPWLRESTILVLLNILYPTLMKFPFLKNHY
IHWSSIVGISLMLTKGEVPSWIIAHFLVEAIASKLKIAKLTQWLKKKNFSQGTLIKFQ
QILVCLAIIVLFAKLDRSSLPFRVLFDHRPFLIDFFTINAIFTVLAVYHRTLKFFFTS
GTKSNKNVGGHEVRNFSQYLGVKNHNDWPISSSNLKHVMDRLNEIHEVTIDDNYANIN
EKIINSYFTKGFFPSLKWTILRQCIEYLFVTKRRRLMGNKLRCIVMLLTFTFVDPTSK
MKISPFFAKFFAKSLVNVYLKKYWHCNFGKYILFFLFQLSIM"
rep_origin 834491..834734
/note="ARS731; Autonomously Replicating Sequence"
/db_xref="SGD:S000118466"
gene complement(<834689..>835903)
/gene="PUS6"
/locus_tag="YGR169C"
/db_xref="GeneID:853079"
mRNA complement(<834689..>835903)
/gene="PUS6"
/locus_tag="YGR169C"
/product="pseudouridine synthase PUS6"
/transcript_id="NM_001181298.3"
/db_xref="GeneID:853079"
CDS complement(834689..835903)
/gene="PUS6"
/locus_tag="YGR169C"
/EC_number="5.4.99.42"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11406626]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:11406626]"
/experiment="EXISTENCE:direct assay:GO:0009982
pseudouridine synthase activity [PMID:11406626]"
/experiment="EXISTENCE:direct assay:GO:0031119 tRNA
pseudouridine synthesis [PMID:11406626]"
/experiment="EXISTENCE:mutant phenotype:GO:0009982
pseudouridine synthase activity [PMID:11406626]"
/experiment="EXISTENCE:mutant phenotype:GO:0031119 tRNA
pseudouridine synthesis [PMID:11406626]"
/note="tRNA:pseudouridine synthase; catalyzes the
conversion of uridine to pseudouridine at position 31 in
cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to
Ala abolishes enzyme activity; not essential for
viability"
/codon_start=1
/product="pseudouridine synthase PUS6"
/protein_id="NP_011685.3"
/db_xref="GeneID:853079"
/db_xref="SGD:S000003401"
/translation="MSTIKVIEVYTQNGLRKVRPYYNRRSAFVKGRWLGKLLIDVLVS
EFKLRPRAYYLDQIRKGTYRLIRDGVPLVPDHLMTTIIKNHDVLETTTHKHEPPVKQW
CSQEVEAEDLPGRIAGFNIVFEDESILVIDKPSGIPVHPTGQFYQNTITELLKLHGVD
ALPCYRLDKITSGLLILAKNSQSAGEIQKSIRSRDMIKIYLARVKGRFPHSELILDNE
NAAETTFEDTSKVTVEMTPIYSIDPKRQFPVGLSTSKDAITKFYPIRYFSHADETVVA
CKPITGRTHQIRIHLARLGHPIVNDSVYCSHITKYPERLKFITQFPRWENQQDLDAEE
LKVRFQKFVDETKNNCRTMETFCPECHTVDLKDPVLSDLELWLHAWKYEEINGKFKFK
TDLPKWAQLDNS"
gene complement(<836387..>836665)
/gene="LSO2"
/locus_tag="YGR169C-A"
/gene_synonym="YGR169C-C"
/db_xref="GeneID:1466460"
mRNA complement(<836387..>836665)
/gene="LSO2"
/locus_tag="YGR169C-A"
/gene_synonym="YGR169C-C"
/product="Lso2p"
/transcript_id="NM_001184531.3"
/db_xref="GeneID:1466460"
CDS complement(836387..836665)
/gene="LSO2"
/locus_tag="YGR169C-A"
/gene_synonym="YGR169C-C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:26450372]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:26450372]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:30208026]"
/experiment="EXISTENCE:mutant phenotype:GO:0002182
cytoplasmic translational elongation [PMID:30208026]"
/experiment="EXISTENCE:mutant phenotype:GO:0006879
intracellular iron ion homeostasis [PMID:26450372]"
/experiment="EXISTENCE:mutant phenotype:GO:0031670
cellular response to nutrient [PMID:30208026]"
/note="Ribosome-associated protein; required for global
translational elongation after recovery from stationary
phase due to starvation; crosslinks to the 25S rRNA near
the A site that overlaps the GTPase activation center, and
to tRNAs; possible role in the iron deprivation response;
localizes to the nucleus and cytoplasm, with enhanced
nuclear localization under iron-replete conditions; null
mutant exhibits slow growth during iron deprivation;
orthologous to human CCDC124"
/codon_start=1
/product="Lso2p"
/protein_id="NP_878080.3"
/db_xref="GeneID:1466460"
/db_xref="SGD:S000028521"
/translation="MGKRFSESAAKKAAGLARKRDQAHAKQRAQMEQLEAEEASKWEQ
GSRKENAKKLEEEQKRQEKARAKKERDALLTAEEEQLGKGGKGKRKMK"
gene <837142..>840558
/gene="PSD2"
/locus_tag="YGR170W"
/db_xref="GeneID:853080"
mRNA <837142..>840558
/gene="PSD2"
/locus_tag="YGR170W"
/product="phosphatidylserine decarboxylase 2"
/transcript_id="NM_001181299.1"
/db_xref="GeneID:853080"
CDS 837142..840558
/gene="PSD2"
/locus_tag="YGR170W"
/EC_number="4.1.1.65"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:20016005]"
/experiment="EXISTENCE:direct assay:GO:0006656
phosphatidylcholine biosynthetic process [PMID:6427211]"
/experiment="EXISTENCE:genetic interaction:GO:0004609
phosphatidylserine decarboxylase activity [PMID:14660568]"
/note="Phosphatidylserine decarboxylase of the Golgi and
vacuolar membranes; converts phosphatidylserine to
phosphatidylethanolamine; controls vacuolar membrane
phospholipid content by regulating phospholipids in
compartments that will eventually give rise to the
vacuole; loss of Psd2p causes a specific reduction in
vacuolar membrane PE levels while total PE levels are not
significantly affected"
/codon_start=1
/product="phosphatidylserine decarboxylase 2"
/protein_id="NP_011686.1"
/db_xref="GeneID:853080"
/db_xref="SGD:S000003402"
/translation="MRIIKGRKRGKNKKPTLILKIHVIQAENIEALKTFNCNPVCFVT
TNTFYSQKTNKLKNSNTHWNQTLRIKLPRNPTSEWLRIIVYDALPTGAPPTTPSRPRT
TTANTSSSTLSNSGLSSHSHSSRNLNVTSKGNQTSTSINSVSSSATPAPSHSSSSLST
TGPGSTHKNRINSYLYLGEAKISLLDLFKRKDTTTSYKFSIEAQRYHLYDMKGGKDQD
SLNCNFLVGDILLGFKLECNVKRTPTFQAFNAWRNELNTYLGRIDRNKARMRSSSSLP
PPLEDMLSNSSAVSGNEIRREKPYSDTDLAHDEEVNAEDEIDAEESIEDMNSSGSICT
ERRYDIDNDTIFDSISEVVSLNDEELDILNDFEEADHPNVPDINVHDIDEDTRISLSS
MITALDEYDIVEPEDVAKLPAVSENDITSVDDEESENQQESDEEFDIYNEDEREDSDF
QSKEYIGSRLLHLQRGKHNKSYANYLYRRAKSNFFISKKEHAMGVVFMHIGAIKNLPA
LRNRLSKTNYEMDPFIVISFGRRVFKTSWRKHTLNPEFNEYAAFEVFPHETNFAFSIK
VVDKDSFSFNDDVAKCELAWFDMLQQQQHENEWIPYEIPLDLTVEPAHAPKQPVLYSS
FKYVSYPFLKKSFWKEAVDTSVNLERLDIIQVMLYLERLGSFTMADSFELFQHFNKSA
WAGQSITRSQLVEGLQSWRKSTNFKRIWTCPRCMRSCKPTRNARRSKLVLENDLITHF
AICTFSKEHKTLKPSYVSSAFASKRWFSKVLIKLTYGKYALGSNNANILVQDRDTGII
IEEKISAHVKLGMRIIYNGKSPESKKFRSLLKTLSIRQGKKFDSTASAKQIEPFIKFH
SLDLSQCRDKDFKTFNEFFYRKLKPGSRLPESNNKEILFSPADSRCTVFPTIQESKEI
WVKGRKFSIKKLANNYNPETFNDNNCSIGIFRLAPQDYHRFHSPCNGTIGKPVYVDGE
YYTVNPMAVRSELDVFGENIRVIIPIDSPQFGKLLYIPIGAMMVGSILLTCKENDVVE
SGQELGYFKFGGSTIIIIIPHNNFMFDSDLVKNSSERIETLVKVGMSIGHTSNVNELK
RIRIKVDDPKKIERIKRTISVSDENAKSTGNVTWEYHTLREMMNKDFAGL"
gene complement(<840824..>842551)
/gene="MSM1"
/locus_tag="YGR171C"
/db_xref="GeneID:853081"
mRNA complement(<840824..>842551)
/gene="MSM1"
/locus_tag="YGR171C"
/product="methionine--tRNA ligase MSM1"
/transcript_id="NM_001181300.1"
/db_xref="GeneID:853081"
CDS complement(840824..842551)
/gene="MSM1"
/locus_tag="YGR171C"
/EC_number="6.1.1.10"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:2645139|PMID:24769239|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:mutant phenotype:GO:0004825
methionine-tRNA ligase activity [PMID:2645139]"
/experiment="EXISTENCE:mutant phenotype:GO:0005739
mitochondrion [PMID:2645139]"
/experiment="EXISTENCE:mutant phenotype:GO:0006431
methionyl-tRNA aminoacylation [PMID:2645139]"
/note="Mitochondrial methionyl-tRNA synthetase (MetRS);
functions as a monomer in mitochondrial protein synthesis;
functions similarly to cytoplasmic MetRS although the
cytoplasmic form contains a zinc-binding domain not found
in Msm1p"
/codon_start=1
/product="methionine--tRNA ligase MSM1"
/protein_id="NP_011687.1"
/db_xref="GeneID:853081"
/db_xref="SGD:S000003403"
/translation="MQCRSIVHRLYSKVSHVTTPIFYPNAKPHLGHLYSSLLSDVYHR
WQLFKGNLSFFTTGTDEHGLKIQCASESNGFDQPKKFVDKLYPEFVQLDKIYGINYTR
FIRTTDPDHIENVMKLWELCLKNGYIYMGEHKGWYSISDETFYPESKVIKDPKNDGKY
LNTESKNEVVYQSETNYFFRLSLFNKKIVDHIRKNPDFIFPASKRDQILKELETGGTL
PDLSISRPSARLKWGIPTPNDPSQKVYVWFDALCNYLSSIGGIPSILSNATEVVSRHY
SDKSNVKGQLLIPYPKEVQRNTIHVIGKDIAKFHTVYWPSFLLAAGLPLPRQIVVHGH
WLCNGMKMSKSLGNVVDPIDMARYYGADIVRWFLLENSKLEEDGDFQEAKLYETRELL
VSKWGNLINRCCGSKFNIERAVMKFSDKANFQFQEIFQNEPIVSERIENLAKLLNKSQ
EVFDEKIAIFQYPQLLRHVWSIINDANTLVQNSKPWERELDQQDNIIFLAMETSRILS
ILCQSIIPSLSQSFLDRIDVSKEKRTINYARLGSDKTYGKQSNKKGREVPLKKIPFRL
QEEQTNMRS"
gene complement(<842845..>843591)
/gene="YIP1"
/locus_tag="YGR172C"
/db_xref="GeneID:853082"
mRNA complement(<842845..>843591)
/gene="YIP1"
/locus_tag="YGR172C"
/product="transporter YIP1"
/transcript_id="NM_001181301.3"
/db_xref="GeneID:853082"
CDS complement(842845..843591)
/gene="YIP1"
/locus_tag="YGR172C"
/experiment="EXISTENCE:direct assay:GO:0000139 Golgi
membrane [PMID:9724632]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:14557247|PMID:15659647]"
/experiment="EXISTENCE:direct assay:GO:0030134
COPII-coated ER to Golgi transport vesicle
[PMID:15659647]"
/experiment="EXISTENCE:mutant phenotype:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:15659647]"
/experiment="EXISTENCE:mutant phenotype:GO:0016192
vesicle-mediated transport [PMID:9724632]"
/experiment="EXISTENCE:mutant phenotype:GO:0048280 vesicle
fusion with Golgi apparatus [PMID:12657649]"
/experiment="EXISTENCE:physical interaction:GO:0030134
COPII-coated ER to Golgi transport vesicle
[PMID:15659647]"
/note="Integral membrane protein; required for the
biogenesis of ER-derived COPII transport vesicles;
interacts with Yif1p and Yos1p; localizes to the Golgi,
the ER, and COPII vesicles; human homolog YIPF5 can
complement yeast yip1 mutant"
/codon_start=1
/product="transporter YIP1"
/protein_id="NP_011688.3"
/db_xref="GeneID:853082"
/db_xref="SGD:S000003404"
/translation="MSFYNTSNNANNGGGFYQPSAQFAVPQGSMSFQNTVGSSNTGND
NNLGVAPDPLPVGILHALSTKGYPHEPPLLEEIGINFDHIITKTKMVLIPIRFGSGVP
QEILNDSDLAGPLIFFLLFGLFLLMAGKVHFGYIYGVALFGTISLHNLSKLMSNNDTS
TQTNLQFFNTASILGYCFLPLCFLSLLGIFHGLNNTTGYVVSVLFVIWSTWTSSGFLN
SLLQLQNARLLIAYPLLIFYSVFALMVIFV"
gene <843854..>844960
/gene="RBG2"
/locus_tag="YGR173W"
/gene_synonym="GIR1"
/db_xref="GeneID:853083"
mRNA <843854..>844960
/gene="RBG2"
/locus_tag="YGR173W"
/gene_synonym="GIR1"
/product="Rbg2p"
/transcript_id="NM_001181302.1"
/db_xref="GeneID:853083"
CDS 843854..844960
/gene="RBG2"
/locus_tag="YGR173W"
/gene_synonym="GIR1"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0002181
cytoplasmic translation [PMID:21076151]"
/experiment="EXISTENCE:genetic interaction:GO:1903833
positive regulation of cellular response to amino acid
starvation [PMID:23899355]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:23899355]"
/note="Protein with a role in translation; forms a complex
with Gir2p; has similarity to mammalian developmentally
regulated GTP-binding protein"
/codon_start=1
/product="Rbg2p"
/protein_id="NP_011689.1"
/db_xref="GeneID:853083"
/db_xref="SGD:S000003405"
/translation="MGIIDKIKAIEEEMARTQKNKATEHHLGLLKGKLARYRQQLLAD
EAGSGGGGGSGFEVAKSGDARVVLIGYPSVGKSSLLGKITTTKSEIAHYAFTTLTSVP
GVLKYQGAEIQIVDLPGIIYGASQGKGRGRQVVATARTADLVLMVLDATKSEHQRASL
EKELENVGIRLNKEKPNIYYKKKETGGVKVTFTSPPKTNLTEQAIKMILRDYRIHNAE
VLVRDDQCTIDDFIDVINEQHRNYVKCLYVYNKIDAVSLEEVDKLAREPNTVVMSCEM
DLGLQDVVEEIWYQLNLSRVYTKKRGVRPVFDDPLVVRNNSTIGDLCHGIHRDFKDKF
KYALVWGSSAKHSPQKCGLNHRIDDEDVVSLFAK"
repeat_region 845281..845602
/note="Ty3 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006981"
gene 845649..845719
/locus_tag="YNCG0039W"
/db_xref="GeneID:853084"
tRNA 845649..845719
/locus_tag="YNCG0039W"
/product="tRNA-Gly"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0030371 translation
repressor activity [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043555 regulation
of translation in response to stress [PMID:27609601]"
/note="Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:853084"
/db_xref="SGD:S000006581"
gene complement(<845893..>846405)
/gene="CBP4"
/locus_tag="YGR174C"
/db_xref="GeneID:853085"
mRNA complement(<845893..>846405)
/gene="CBP4"
/locus_tag="YGR174C"
/product="Cbp4p"
/transcript_id="NM_001181303.3"
/db_xref="GeneID:853085"
CDS complement(845893..846405)
/gene="CBP4"
/locus_tag="YGR174C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:8063753|PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0031966
mitochondrial membrane [PMID:8063753]"
/experiment="EXISTENCE:mutant phenotype:GO:0034551
mitochondrial respiratory chain complex III assembly
[PMID:15759116|PMID:8063753]"
/note="Mitochondrial protein required for assembly of
cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p
complex and newly synthesized cytochrome b (Cobp) to
promote assembly of Cobp into the cytochrome bc1 complex"
/codon_start=1
/product="Cbp4p"
/protein_id="NP_011690.3"
/db_xref="GeneID:853085"
/db_xref="SGD:S000003406"
/translation="MQCAITPREAVIAKQRQYKHYLGMERPLWVRWLKVYAIGGAIIG
SGFLLFKYTTPTDQQLISQLSPELRLQYEREKKLRQSEQQALMKIVKETSQSDDPIWK
TGPLQSPWERNGDNVQSRDHFAKVRAEEVQKEELARIRNELSQLRSETEEKTKEIVQD
KQVKSWWRFW"
gene <846655..>846741
/locus_tag="YGR174W-A"
/db_xref="GeneID:1466461"
mRNA <846655..>846741
/locus_tag="YGR174W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184595.3"
/db_xref="GeneID:1466461"
CDS 846655..846741
/locus_tag="YGR174W-A"
/note="hypothetical protein; predicted to have a role in
cell budding based on computational 'guilt by association'
analysis"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878081.3"
/db_xref="GeneID:1466461"
/db_xref="SGD:S000028639"
/translation="MNLNAYFEAYQAIFPFLLEAFLRKEQKV"
gene complement(<846933..>848423)
/gene="ERG1"
/locus_tag="YGR175C"
/db_xref="GeneID:853086"
mRNA complement(<846933..>848423)
/gene="ERG1"
/locus_tag="YGR175C"
/product="squalene monooxygenase"
/transcript_id="NM_001181304.1"
/db_xref="GeneID:853086"
CDS complement(846933..848423)
/gene="ERG1"
/locus_tag="YGR175C"
/EC_number="1.14.14.17"
/experiment="EXISTENCE:direct assay:GO:0004506 squalene
monooxygenase activity [PMID:8358382|PMID:9450962]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:9450962|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005811 lipid
droplet [PMID:9450962|PMID:24868093]"
/experiment="EXISTENCE:direct assay:GO:0006696 ergosterol
biosynthetic process [PMID:8358382]"
/experiment="EXISTENCE:mutant phenotype:GO:0004506
squalene monooxygenase activity [PMID:200835]"
/experiment="EXISTENCE:mutant phenotype:GO:0006696
ergosterol biosynthetic process [PMID:200835]"
/note="Squalene epoxidase; catalyzes epoxidation of
squalene to 2,3-oxidosqualene; plays essential role in
ergosterol-biosynthesis pathway and is specific target of
antifungal drug terbinafine; directed to ER via guided
entry of tail-anchored proteins (GET) pathway; degradation
by ERAD triggered by membrane lipid saturation; human SQLE
functionally complements the lethality of the erg1 null
mutation"
/codon_start=1
/product="squalene monooxygenase"
/protein_id="NP_011691.1"
/db_xref="GeneID:853086"
/db_xref="SGD:S000003407"
/translation="MSAVNVAPELINADNTITYDAIVIGAGVIGPCVATGLARKGKKV
LIVERDWAMPDRIVGELMQPGGVRALRSLGMIQSINNIEAYPVTGYTVFFNGEQVDIP
YPYKADIPKVEKLKDLVKDGNDKVLEDSTIHIKDYEDDERERGVAFVHGRFLNNLRNI
TAQEPNVTRVQGNCIEILKDEKNEVVGAKVDIDGRGKVEFKAHLTFICDGIFSRFRKE
LHPDHVPTVGSSFVGMSLFNAKNPAPMHGHVILGSDHMPILVYQISPEETRILCAYNS
PKVPADIKSWMIKDVQPFIPKSLRPSFDEAVSQGKFRAMPNSYLPARQNDVTGMCVIG
DALNMRHPLTGGGMTVGLHDVVLLIKKIGDLDFSDREKVLDELLDYHFERKSYDSVIN
VLSVALYSLFAADSDNLKALQKGCFKYFQRGGDCVNKPVEFLSGVLPKPLQLTRVFFA
VAFYTIYLNMEERGFLGLPMALLEGIMILITAIRVFTPFLFGELIG"
gene complement(<848829..>850436)
/gene="ATF2"
/locus_tag="YGR177C"
/db_xref="GeneID:853088"
mRNA complement(<848829..>850436)
/gene="ATF2"
/locus_tag="YGR177C"
/product="alcohol O-acetyltransferase"
/transcript_id="NM_001181306.1"
/db_xref="GeneID:853088"
CDS complement(848829..850436)
/gene="ATF2"
/locus_tag="YGR177C"
/EC_number="2.3.1.84"
/experiment="EXISTENCE:direct assay:GO:0004026 alcohol
O-acetyltransferase activity [PMID:9836419]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:18034159]"
/experiment="EXISTENCE:genetic interaction:GO:1901089
acetate ester metabolic process involved in fermentation
[PMID:12957907]"
/experiment="EXISTENCE:mutant phenotype:GO:0008202 steroid
metabolic process [PMID:10103065]"
/experiment="EXISTENCE:mutant phenotype:GO:0009636
response to toxic substance [PMID:18034159]"
/note="Alcohol acetyltransferase; may play a role in
steroid detoxification; forms volatile esters during
fermentation, which is important for brewing and
winemaking"
/codon_start=1
/product="alcohol O-acetyltransferase"
/protein_id="NP_011693.1"
/db_xref="GeneID:853088"
/db_xref="SGD:S000003409"
/translation="MEDIEGYEPHITQELIDRGHARRMGHLENYFAVLSRQKMYSNFT
VYAELNKGVNKRQLMLVLKVLLQKYSTLAHTIIPKHYPHHEAYYSSEEYLSKPFPQHD
FIKVISHLEFDDLIMNNQPEYREVMEKISEQFKKDDFKVTNRLIELISPVIIPLGNPK
RPNWRLICLPGKDTDGFETWKNFVYVTNHCGSDGVSGSNFFKDLALLFCKIEEKGFDY
DEEFIEDQVIIDYDRDYTEISKLPKPITDRIDYKPALTSLPKFFLTTFIYEHCNFKTS
SESTLTARYSPSSNANASYNYLLHFSTKQVEQIRAQIKKNVHDGCTLTPFIQACFLVA
LYRLDKLFTKSLLEYGFDVAIPSNARRFLPNDEELRDSYKYGSNVGGSHYAYLISSFD
IPEGDNDKFWSLVEYYYDRFLESYDNGDHLIGLGVLQLDFIVENKNIDSLLANSYLHQ
QRGGAIISNTGLVSQDTTKPYYVRDLIFSQSAGALRFAFGLNVCSTNVNGMNMDMSVV
QGTLRDRGEWESFCKLFYQTIGEFASL"
gene complement(<851047..>853215)
/gene="PBP1"
/locus_tag="YGR178C"
/gene_synonym="MRS16"
/db_xref="GeneID:853089"
mRNA complement(<851047..>853215)
/gene="PBP1"
/locus_tag="YGR178C"
/gene_synonym="MRS16"
/product="Pbp1p"
/transcript_id="NM_001181307.3"
/db_xref="GeneID:853089"
CDS complement(851047..853215)
/gene="PBP1"
/locus_tag="YGR178C"
/gene_synonym="MRS16"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11914276|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922|PMID:14562095|PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405|PMID:18981231]"
/experiment="EXISTENCE:direct assay:GO:0044877
protein-containing complex binding [PMID:30982600]"
/experiment="EXISTENCE:mutant phenotype:GO:0034063 stress
granule assembly [PMID:18981231]"
/experiment="EXISTENCE:mutant phenotype:GO:0042149
cellular response to glucose starvation [PMID:18981231]"
/experiment="EXISTENCE:mutant phenotype:GO:0043007
maintenance of rDNA [PMID:25073155]"
/experiment="EXISTENCE:mutant phenotype:GO:0045727
positive regulation of translation [PMID:15082763]"
/experiment="EXISTENCE:mutant phenotype:GO:1901524
regulation of mitophagy [PMID:30982600]"
/experiment="EXISTENCE:mutant phenotype:GO:1904262
negative regulation of TORC1 signaling [PMID:30982600]"
/experiment="EXISTENCE:physical interaction:GO:0045727
positive regulation of translation [PMID:15082763]"
/note="Component of glucose deprivation induced stress
granules; involved in P-body-dependent granule assembly;
regulates TORC1 signaling and autophagy; interacts with
Pab1p to regulate mRNA polyadenylation; interacts with
Mkt1p to regulate HO translation; regulates expression of
genes involved in gluconeogenesis and mitochondrial
function through multiple mechanisms"
/codon_start=1
/product="Pbp1p"
/protein_id="NP_011694.3"
/db_xref="GeneID:853089"
/db_xref="SGD:S000003410"
/translation="MKGNFRKRDSSTNSRKGGNSDSNYTNGGVPNQNNSSMFYENPEI
TRNFDDRQDYLLANSIGSDVTVTVTSGVKYTGLLVSCNLESTNGIDVVLRFPRVADSG
VSDSVDDLAKTLGETLLIHGEDVAELELKNIDLSLDEKWENSKAQETTPARTNIEKER
VNGESNEVTKFRTDVDISGSGREIKERKLEKWTPEEGAEHFDINKGKALEDDSASWDQ
FAVNEKKFGVKSTFDEHLYTTKINKDDPNYSKRLQEAERIAKEIESQGTSGNIHIAED
RGIIIDDSGLDEEDLYSGVDRRGDELLAALKSNSKPNSNKGNRYVPPTLRQQPHHMDP
AIISSSNSNKNENAVSTDTSTPAAAGAPEGKPPQKTSKNKKSLSSKEAQIEELKKFSE
KFKVPYDIPKDMLEVLKRSSSTLKSNSSLPPKPISKTPSAKTVSPTTQISAGKSESRR
SGSNISQGQSSTGHTTRSSTSLRRRNHGSFFGAKNPHTNDAKRVLFGKSFNMFIKSKE
AHDEKKKGDDASENMEPFFIEKPYFTAPTWLNTIEESYKTFFPDEDTAIQEAQTRFQQ
RQLNSMGNAVPGMNPAMGMNMGGMMGFPMGGPSASPNPMMNGFAAGSMGMYMPFQPQP
MFYHPSMPQMMPVMGSNGAEEGGGNISPHVPAGFMAAGPGAPMGAFGYPGGIPFQGMM
GSGPSGMPANGSAMHSHGHSRNYHQTSHHGHHNSSTSGHK"
gene complement(<853675..>854895)
/gene="OKP1"
/locus_tag="YGR179C"
/db_xref="GeneID:853090"
mRNA complement(<853675..>854895)
/gene="OKP1"
/locus_tag="YGR179C"
/product="Okp1p"
/transcript_id="NM_001181308.1"
/db_xref="GeneID:853090"
CDS complement(853675..854895)
/gene="OKP1"
/locus_tag="YGR179C"
/experiment="EXISTENCE:direct assay:GO:0000776 kinetochore
[PMID:10323865]"
/experiment="EXISTENCE:direct assay:GO:0000817 COMA
complex [PMID:14633972]"
/experiment="EXISTENCE:mutant phenotype:GO:0008608
attachment of spindle microtubules to kinetochore
[PMID:19597337]"
/note="Outer kinetochore protein required for accurate
chromosome segregation; component of COMA (Ctf19p, Okp1p,
Mcm21p, Ame1p) a kinetochore sub-complex which functions
as a platform for kinetochore assembly; orthologous to
human centromere constitutive-associated network (CCAN)
subunit CENP-Q and fission yeast fta7"
/codon_start=1
/product="Okp1p"
/protein_id="NP_011695.1"
/db_xref="GeneID:853090"
/db_xref="SGD:S000003411"
/translation="MAADRDNFLQNIENDSINNGQAMDLSPNRSSSESDSSILMNVND
IKTLRLDVAPEAKSTQSKKSLFYENSDDAEEGEIEERTNKEEGQYHHKGSKQLRFEVG
KESTGKLQSHLSDGSATSGEGNVRPWEFRKVIQAEYRERLPRNYELKHWKKPSKIMIG
SILRLLETNTVSALDSVFEKYEKEMNQMTHGDNNEVKRIYSKKERLLEIILTKIKKKL
RQAKFPSRISERDLDIEYIYSKRQFIQNRYSQELQNNERLEAILSREQNLLEETRKLC
MNLKTNNKKRLTEKLIQKDLHPVLNKAMEYTYGLESTNGFMHPDGPVTFRNDSHELNL
MLNDPIKSTADVRLDKEEVLSLLPSLKEYTKKSKELKETMGQMISDSHEEEIKEVFVP
HHESHQDKTEEDIH"
gene complement(<855264..>856301)
/gene="RNR4"
/locus_tag="YGR180C"
/gene_synonym="CRT3; PSO3"
/db_xref="GeneID:853091"
mRNA complement(<855264..>856301)
/gene="RNR4"
/locus_tag="YGR180C"
/gene_synonym="CRT3; PSO3"
/product="ribonucleotide-diphosphate reductase subunit
RNR4"
/transcript_id="NM_001181309.3"
/db_xref="GeneID:853091"
CDS complement(855264..856301)
/gene="RNR4"
/locus_tag="YGR180C"
/gene_synonym="CRT3; PSO3"
/EC_number="1.17.4.1"
/experiment="EXISTENCE:direct assay:GO:0004748
ribonucleoside-diphosphate reductase activity, thioredoxin
disulfide as acceptor [PMID:10716984|PMID:10535923]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12732713|PMID:9315670|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922|PMID:12732713]"
/experiment="EXISTENCE:direct assay:GO:0005971
ribonucleoside-diphosphate reductase complex
[PMID:10716984]"
/experiment="EXISTENCE:direct assay:GO:0009263
deoxyribonucleotide biosynthetic process
[PMID:10535923|PMID:5459124]"
/experiment="EXISTENCE:genetic interaction:GO:0004748
ribonucleoside-diphosphate reductase activity, thioredoxin
disulfide as acceptor [PMID:9315671|PMID:9315670]"
/experiment="EXISTENCE:mutant phenotype:GO:0004748
ribonucleoside-diphosphate reductase activity, thioredoxin
disulfide as acceptor [PMID:16285741|PMID:9315671]"
/experiment="EXISTENCE:physical interaction:GO:0005971
ribonucleoside-diphosphate reductase complex
[PMID:9315670]"
/note="Ribonucleotide-diphosphate reductase (RNR) small
subunit; the RNR complex catalyzes the rate-limiting step
in dNTP synthesis and is regulated by DNA replication and
DNA damage checkpoint pathways via localization of the
small subunits; relocalizes from nucleus to cytoplasm upon
DNA replication stress; RNR4 has a paralog, RNR2, that
arose from the whole genome duplication"
/codon_start=1
/product="ribonucleotide-diphosphate reductase subunit
RNR4"
/protein_id="NP_011696.3"
/db_xref="GeneID:853091"
/db_xref="SGD:S000003412"
/translation="MEAHNQFLKTFQKERHDMKEAEKDEILLMENSRRFVMFPIKYHE
IWAAYKKVEASFWTAEEIELAKDTEDFQKLTDDQKTYIGNLLALSISSDNLVNKYLIE
NFSAQLQNPEGKSFYGFQIMMENIYSEVYSMMVDAFFKDPKNIPLFKEIANLPEVKHK
AAFIERWISNDDSLYAERLVAFAAKEGIFQAGNYASMFWLTDKKIMPGLAMANRNICR
DRGAYTDFSCLLFAHLRTKPNPKIIEKIITEAVEIEKEYYSNSLPVEKFGMDLKSIHT
YIEFVADGLLQGFGNEKYYNAVNPFEFMEDVATAGKTTFFEKKVSDYQKASDMSKSAT
PSKEINFDDDF"
gene complement(857378..857491)
/locus_tag="YNCG0040C"
/db_xref="GeneID:853092"
tRNA complement(join(857378..857421,857454..857491))
/locus_tag="YNCG0040C"
/product="tRNA-Leu"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:853092"
/db_xref="SGD:S000006641"
gene <858287..>858604
/gene="TIM13"
/locus_tag="YGR181W"
/db_xref="GeneID:853093"
mRNA <858287..>858604
/gene="TIM13"
/locus_tag="YGR181W"
/product="protein translocase subunit TIM13"
/transcript_id="NM_001181310.3"
/db_xref="GeneID:853093"
CDS 858287..858604
/gene="TIM13"
/locus_tag="YGR181W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005758
mitochondrial intermembrane space
[PMID:10397776|PMID:10051608]"
/experiment="EXISTENCE:direct assay:GO:0042719
mitochondrial intermembrane space chaperone complex
[PMID:12221072]"
/experiment="EXISTENCE:direct assay:GO:0045039 protein
insertion into mitochondrial inner membrane
[PMID:10051608|PMID:10469659]"
/experiment="EXISTENCE:direct assay:GO:0140318 protein
transporter activity [PMID:10469659|PMID:10051608]"
/experiment="EXISTENCE:mutant phenotype:GO:0045039 protein
insertion into mitochondrial inner membrane
[PMID:10469659]"
/experiment="EXISTENCE:mutant phenotype:GO:0140318 protein
transporter activity [PMID:10469659]"
/experiment="EXISTENCE:physical interaction:GO:0042719
mitochondrial intermembrane space chaperone complex
[PMID:10051608]"
/note="Mitochondrial intermembrane space protein; forms a
complex with Tim8p that delivers a subset of hydrophobic
proteins to the TIM22 complex for insertion into the inner
membrane"
/codon_start=1
/product="protein translocase subunit TIM13"
/protein_id="NP_011697.3"
/db_xref="GeneID:853093"
/db_xref="SGD:S000003413"
/translation="MGLSSIFGGGAPSQQKEAATTAKTTPNPIAKELKNQIAQELAVA
NATELVNKISENCFEKCLTSPYATRNDACIDQCLAKYMRSWNVISKAYISRIQNASAS
GEI"
gene complement(<859063..>859476)
/gene="QCR9"
/locus_tag="YGR183C"
/gene_synonym="UCR9"
/db_xref="GeneID:853095"
mRNA complement(join(<859063..859260,859474..>859476))
/gene="QCR9"
/locus_tag="YGR183C"
/gene_synonym="UCR9"
/product="ubiquinol--cytochrome-c reductase subunit 9"
/transcript_id="NM_001181312.1"
/db_xref="GeneID:853095"
CDS complement(join(859063..859260,859474..859476))
/gene="QCR9"
/locus_tag="YGR183C"
/gene_synonym="UCR9"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:10873857]"
/experiment="EXISTENCE:direct assay:GO:0045275 respiratory
chain complex III [PMID:10873857]"
/experiment="EXISTENCE:mutant phenotype:GO:0006122
mitochondrial electron transport, ubiquinol to cytochrome
c [PMID:1332881]"
/experiment="EXISTENCE:mutant phenotype:GO:0008121
quinol-cytochrome-c reductase activity [PMID:1332881]"
/experiment="EXISTENCE:mutant phenotype:GO:0009060 aerobic
respiration [PMID:1332881]"
/experiment="EXISTENCE:mutant phenotype:GO:0034551
mitochondrial respiratory chain complex III assembly
[PMID:8389362]"
/note="Subunit 9 of ubiquinol cytochrome-c reductase
(Complex III); Complex III is a component of the
mitochondrial inner membrane electron transport chain;
required for electron transfer at the ubiquinol oxidase
site of the complex"
/codon_start=1
/product="ubiquinol--cytochrome-c reductase subunit 9"
/protein_id="NP_011699.1"
/db_xref="GeneID:853095"
/db_xref="SGD:S000003415"
/translation="MSFSSLYKTFFKRNAVFVGTIFAGAFVFQTVFDTAITSWYENHN
KGKLWKDVKARIAAGDGDDDDE"
gene complement(<859901..>865753)
/gene="UBR1"
/locus_tag="YGR184C"
/gene_synonym="PTR1"
/db_xref="GeneID:853096"
mRNA complement(<859901..>865753)
/gene="UBR1"
/locus_tag="YGR184C"
/gene_synonym="PTR1"
/product="E3 ubiquitin-protein ligase UBR1"
/transcript_id="NM_001181313.1"
/db_xref="GeneID:853096"
CDS complement(859901..865753)
/gene="UBR1"
/locus_tag="YGR184C"
/gene_synonym="PTR1"
/EC_number="2.3.2.27"
/experiment="EXISTENCE:direct assay:GO:0000209 protein
polyubiquitination [PMID:20462952]"
/experiment="EXISTENCE:direct assay:GO:0006513 protein
monoubiquitination [PMID:20462952]"
/experiment="EXISTENCE:genetic interaction:GO:0005737
cytoplasm [PMID:20080635]"
/experiment="EXISTENCE:genetic interaction:GO:0034620
cellular response to unfolded protein [PMID:20462952]"
/experiment="EXISTENCE:genetic interaction:GO:0036503 ERAD
pathway [PMID:23988329]"
/experiment="EXISTENCE:genetic interaction:GO:0071629
cytoplasm protein quality control by the
ubiquitin-proteasome system [PMID:20462952]"
/experiment="EXISTENCE:mutant phenotype:GO:0000209 protein
polyubiquitination [PMID:10581257|PMID:2209542]"
/experiment="EXISTENCE:mutant phenotype:GO:0004842
ubiquitin-protein transferase activity [PMID:10581257]"
/experiment="EXISTENCE:mutant phenotype:GO:0005737
cytoplasm [PMID:20080635]"
/experiment="EXISTENCE:mutant phenotype:GO:0034620
cellular response to unfolded protein [PMID:20462952]"
/experiment="EXISTENCE:mutant phenotype:GO:0036503 ERAD
pathway [PMID:23988329]"
/experiment="EXISTENCE:mutant phenotype:GO:0071596
ubiquitin-dependent protein catabolic process via the
N-end rule pathway [PMID:2209542]"
/experiment="EXISTENCE:mutant phenotype:GO:0071629
cytoplasm protein quality control by the
ubiquitin-proteasome system
[PMID:27448207|PMID:22670231|PMID:19041308|PMID:20080635|P
MID:20462952]"
/experiment="EXISTENCE:mutant phenotype:GO:0072671
mitochondria-associated ubiquitin-dependent protein
catabolic process [PMID:32118579]"
/experiment="EXISTENCE:mutant phenotype:GO:0090089
regulation of dipeptide transport [PMID:9427760]"
/experiment="EXISTENCE:mutant phenotype:GO:0120174
stress-induced homeostatically regulated protein
degradation pathway [PMID:29861160]"
/experiment="EXISTENCE:mutant phenotype:GO:1990116
ribosome-associated ubiquitin-dependent protein catabolic
process [PMID:23358411]"
/experiment="EXISTENCE:physical interaction:GO:0008540
proteasome regulatory particle, base subcomplex
[PMID:10688918]"
/experiment="EXISTENCE:physical interaction:GO:1904855
proteasome regulatory particle binding [PMID:10688918]"
/experiment="EXISTENCE:physical interaction:GO:1990303
UBR1-RAD6 ubiquitin ligase complex
[PMID:8505328|PMID:1651502]"
/note="E3 Ub-ligase (N-recognin); recognizes and
ubiquitinates N-end rule pathway substrates in complex
with Rad6p; Roq1p-bound form accelerates degradation of
misfolded and native ER membrane and cytosolic proteins as
part of the stress-induced homeostatically regulated
protein degradation (SHRED) pathway; role in ER-associated
protein degradation; modifies Cup9p to regulate peptide
transport; mutation in human UBR1 causes
Johansson-Blizzard Syndrome (JBS)"
/codon_start=1
/product="E3 ubiquitin-protein ligase UBR1"
/protein_id="NP_011700.1"
/db_xref="GeneID:853096"
/db_xref="SGD:S000003416"
/translation="MSVADDDLGSLQGHIRRTLRSIHNLPYFRYTRGPTERADMSRAL
KEFIYRYLYFVISNSGENLPTLFNAHPKQKLSNPELTVFPDSLEDAVDIDKITSQQTI
PFYKIDESRIGDVHKHTGRNCGRKFKIGEPLYRCHECGCDDTCVLCIHCFNPKDHVNH
HVCTDICTEFTSGICDCGDEEAWNSPLHCKAEEQENDISEDPATNADIKEEDVWNDSV
NIALVELVLAEVFDYFIDVFNQNIEPLPTIQKDITIKLREMTQQGKMYERAQFLNDLK
YENDYMFDGTTTAKTSPSNSPEASPSLAKIDPENYTVIIYNDEYHNYSQATTALRQGV
PDNVHIDLLTSRIDGEGRAMLKCSQDLSSVLGGFFAVQTNGLSATLTSWSEYLHQETC
KYIILWITHCLNIPNSSFQTTFRNMMGKTLCSEYLNATECRDMTPVVEKYFSNKFDKN
DPYRYIDLSILADGNQIPLGHHKILPESSTHSLSPLINDVETPTSRTYSNTRLQHILY
FDNRYWKRLRKDIQNVIIPTLASSNLYKPIFCQQVVEIFNHITRSVAYMDREPQLTAI
RECVVQLFTCPTNAKNIFENQSFLDIVWSIIDIFKEFCKVEGGVLIWQRVQKSNLTKS
YSISFKQGLYTVETLLSKVHDPNIPLRPKEIISLLTLCKLFNGAWKIKRKEGEHVLHE
DQNFISYLEYTTSIYSIIQTAEKVSEKSKDSIDSKLFLNAIRIISSFLGNRSLTYKLI
YDSHEVIKFSVSHERVAFMNPLQTMLSFLIEKVSLKDAYEALEDCSDFLKISDFSLRS
VVLCSQIDVGFWVRNGMSVLHQASYYKNNPELGSYSRDIHLNQLAILWERDDIPRIIY
NILDRWELLDWFTGEVDYQHTVYEDKISFIIQQFIAFIYQILTERQYFKTFSSLKDRR
MDQIKNSIIYNLYMKPLSYSKLLRSVPDYLTEDTTEFDEALEEVSVFVEPKGLADNGV
FKLKASLYAKVDPLKLLNLENEFESSATIIKSHLAKDKDEIAKVVLIPQVSIKQLDKD
ALNLGAFTRNTVFAKVVYKLLQVCLDMEDSTFLNELLHLVHGIFRDDELINGKDSIPE
AYLSKPICNLLLSIANAKSDVFSESIVRKADYLLEKMIMKKPNELFESLIASFGNQYV
NDYKDKKLRQGVNLQETEKERKRRLAKKHQARLLAKFNNQQTKFMKEHESEFDEQDND
VDMVGEKVYESEDFTCALCQDSSSTDFFVIPAYHDHSPIFRPGNIFNPNEFMPMWDGF
YNDDEKQAYIDDDVLEALKENGSCGSRKVFVSCNHHIHHNCFKRYVQKKRFSSNAFIC
PLCQTFSNCTLPLCQTSKANTGLSLDMFLESELSLDTLSRLFKPFTEENYRTINSIFS
LMISQCQGFDKAVRKRANFSHKDVSLILSVHWANTISMLEIASRLEKPYSISFFRSRE
QKYKTLKNILVCIMLFTFVIGKPSMEFEPYPQQPDTVWNQNQLFQYIVRSALFSPVSL
RQTVTEALTTFSRQFLRDFLQGLSDAEQVTKLYAKASKIGDVLKVSEQMLFALRTISD
VRMEGLDSESIIYDLAYTFLLKSLLPTIRRCLVFIKVLHELVKDSENETLVINGHEVE
EELEFEDTAEFVNKALKMITEKESLVDLLTTQESIVSHPYLENIPYEYCGIIKLIDLS
KYLNTYVTQSKEIKLREERSQHMKNADNRLDFKICLTCGVKVHLRADRHEMTKHLNKN
CFKPFGAFLMPNSSEVCLHLTQPPSNIFISAPYLNSHGEVGRNAMRRGDLTTLNLKRY
EHLNRLWINNEIPGYISRVMGDEFRVTILSNGFLFAFNREPRPRRIPPTDEDDEDMEE
GEDGFFTEGNDEMDVDDETGQAANLFGVGAEGIAGGGVRDFFQFFENFRNTLQPQGNG
DDDAPQNPPPILQFLGPQFDGATIIRNTNPRNLDEDDSDDNDDSDEREIW"
gene complement(<866336..>867520)
/gene="TYS1"
/locus_tag="YGR185C"
/gene_synonym="TTS1"
/db_xref="GeneID:853097"
mRNA complement(<866336..>867520)
/gene="TYS1"
/locus_tag="YGR185C"
/gene_synonym="TTS1"
/product="tyrosine--tRNA ligase TYS1"
/transcript_id="NM_001181314.3"
/db_xref="GeneID:853097"
CDS complement(866336..867520)
/gene="TYS1"
/locus_tag="YGR185C"
/gene_synonym="TTS1"
/EC_number="6.1.1.1"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0004831
tyrosine-tRNA ligase activity [PMID:8509419|PMID:782885]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11359929]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11359929]"
/experiment="EXISTENCE:direct assay:GO:0006437
tyrosyl-tRNA aminoacylation [PMID:8509419|PMID:782885]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405]"
/experiment="EXISTENCE:direct assay:GO:1990825
sequence-specific mRNA binding [PMID:34039240]"
/experiment="EXISTENCE:mutant phenotype:GO:0005634 nucleus
[PMID:11359929]"
/note="Cytoplasmic tyrosyl-tRNA synthetase; required for
cytoplasmic protein synthesis; interacts with positions 34
and 35 of the tRNATyr anticodon; mutations in human
ortholog YARS are associated with Charcot-Marie-Tooth
(CMT) neuropathies; human ortholog YARS functionally
complements the heat sensitivity of a ts allele; protein
abundance increases in response to DNA replication stress"
/codon_start=1
/product="tyrosine--tRNA ligase TYS1"
/protein_id="NP_011701.3"
/db_xref="GeneID:853097"
/db_xref="SGD:S000003417"
/translation="MSSAATVDPNEAFGLITKNLQEVLNPQIIKDVLEVQKRHLKLYW
GTAPTGRPHCGYFVPMTKLADFLKAGCEVTVLLADLHAFLDNMKAPLEVVNYRAKYYE
LTIKAILRSINVPIEKLKFVVGSSYQLTPDYTMDIFRLSNIVSQNDAKRAGADVVKQV
ANPLLSGLIYPLMQALDEQFLDVDCQFGGVDQRKIFVLAEENLPSLGYKKRAHLMNPM
VPGLAQGGKMSASDPNSKIDLLEEPKQVKKKINSAFCSPGNVEENGLLSFVQYVIAPI
QELKFGTNHFEFFIDRPEKFGGPITYKSFEEMKLAFKEEKLSPPDLKIGVADAINELL
EPIRQEFANNKEFQEASEKGYPVATPQKSKKAKKPKNKGTKYPGATKTNEIATKLEET
KL"
gene <867774..>869981
/gene="TFG1"
/locus_tag="YGR186W"
/gene_synonym="RAP74; SSU71"
/db_xref="GeneID:853098"
mRNA <867774..>869981
/gene="TFG1"
/locus_tag="YGR186W"
/gene_synonym="RAP74; SSU71"
/product="transcription factor IIF subunit TFG1"
/transcript_id="NM_001181315.1"
/db_xref="GeneID:853098"
CDS 867774..869981
/gene="TFG1"
/locus_tag="YGR186W"
/gene_synonym="RAP74; SSU71"
/experiment="EXISTENCE:direct assay:GO:0005674
transcription factor TFIIF complex
[PMID:12242279|PMID:7995524]"
/experiment="EXISTENCE:direct assay:GO:0006367
transcription initiation at RNA polymerase II promoter
[PMID:1331084|PMID:18362165]"
/experiment="EXISTENCE:direct assay:GO:0006368
transcription elongation by RNA polymerase II
[PMID:18362165]"
/experiment="EXISTENCE:direct assay:GO:0072542 protein
phosphatase activator activity [PMID:11904169]"
/experiment="EXISTENCE:genetic interaction:GO:0001174
transcriptional start site selection at RNA polymerase II
promoter
[PMID:15572698|PMID:16147988|PMID:16081422|PMID:7724527]"
/experiment="EXISTENCE:mutant phenotype:GO:0001174
transcriptional start site selection at RNA polymerase II
promoter [PMID:16081422|PMID:15572698|PMID:18362165]"
/experiment="EXISTENCE:mutant phenotype:GO:0006367
transcription initiation at RNA polymerase II promoter
[PMID:18362165]"
/experiment="EXISTENCE:mutant phenotype:GO:0006368
transcription elongation by RNA polymerase II
[PMID:18362165]"
/note="TFIIF (Transcription Factor II) largest subunit;
involved in both transcription initiation and elongation
of RNA polymerase II; homologous to human RAP74"
/codon_start=1
/product="transcription factor IIF subunit TFG1"
/protein_id="NP_011702.1"
/db_xref="GeneID:853098"
/db_xref="SGD:S000003418"
/translation="MSRRNPPGSRNGGGPTNASPFIKRDRMRRNFLRMRMGQNGSNSS
SPGVPNGDNSRGSLVKKDDPEYAEEREKMLLQIGVEADAGRSNVKVKDEDPNEYNEFP
LRAIPKEDLENMRTHLLKFQSKKKINPVTDFHLPVRLHRKDTRNLQFQLTRAEIVQRQ
KEISEYKKKAEQERSTPNSGGMNKSGTVSLNNTVKDGSQTPTVDSVTKDNTANGVNSS
IPTVTGSSVPPASPTTVSAVESNGLSNGSTSAANGLDGNASTANLANGRPLVTKLEDA
GPAEDPTKVGMVKYDGKEVTNEPEFEEGTMDPLADVAPDGGGRAKRGNLRRKTRQLKV
LDENAKKLRFEEFYPWVMEDFDGYNTWVGSYEAGNSDSYVLLSVEDDGSFTMIPADKV
YKFTARNKYATLTIDEAEKRMDKKSGEVPRWLMKHLDNIGTTTTRYDRTRRKLKAVAD
QQAMDEDDRDDNSEVELDYDEEFADDEEAPIIDGNEQENKESEQRIKKEMLQANAMGL
RDEEAPSENEEDELFGEKKIDEDGERIKKALQKTELAALYSSDENEINPYLSESDIEN
KENESPVKKEEDSDTLSKSKRSSPKKQQKKATNAHVHKEPTLRVKSIKNCVIILKGDK
KILKSFPEGEWNPQTTKAVDSSNNASNTVPSPIKQEEGLNSTVAEREETPAPTITEKD
IIEAIGDGKVNIKEFGKFIRRKYPGAENKKLMFAIVKKLCRKVGNDHMELKKE"
gene complement(<870232..>871416)
/gene="HGH1"
/locus_tag="YGR187C"
/db_xref="GeneID:853099"
mRNA complement(<870232..>871416)
/gene="HGH1"
/locus_tag="YGR187C"
/product="Hgh1p"
/transcript_id="NM_001181316.3"
/db_xref="GeneID:853099"
CDS complement(870232..871416)
/gene="HGH1"
/locus_tag="YGR187C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0061770 translation
elongation factor binding [PMID:30876804]"
/experiment="EXISTENCE:mutant phenotype:GO:0006457 protein
folding [PMID:30876804]"
/note="Chaperone protein for translation factor eEF2;
involved in biogenesis of eukaryotic Elongation Factor 2
(Eft1p, Eft2p); green fluorescent protein (GFP)-fusion
protein localizes to the cytoplasm; similar to mammalian
BRP16 (Brain protein 16); relative distribution to the
nucleus increases upon DNA replication stress"
/codon_start=1
/product="Hgh1p"
/protein_id="NP_011703.3"
/db_xref="GeneID:853099"
/db_xref="SGD:S000003419"
/translation="MTSQLNELVEFLHSPQPAVRQIAIDNLVGFSAGPTSKVFKNDSY
RPIKDIIKMIMDPEHGTRVIIQQGVTILVNLSEDKLVRNIILSDDKKFLKFLVWKIVD
LTNPNADIMCILLSNLAKDDGILAVLNIKRNSSGEEVDDGLKLAALNKEVFKSLRAMD
CLMDCFVKGYDKKLTKYASFNYLAFFFADISRFKLGRMYFIEEQEYDGVVPISKLLVF
TEKYDAKVRREGVASTIKNSLFDSETHERLLKDEKINLLPYILLPIASAKDSEIDEED
MFNLPDELQLLPEDKERDPIPAIICCHLESILLLCTTHAGREYLRDKSVYPLVRELHK
NVENEDIGELCYRIVNMLMRGEPGAGAVEEMPSKNAEEEEEEESEEEEDDDEEDEIVE
VA"
gene complement(<872044..>875109)
/gene="BUB1"
/locus_tag="YGR188C"
/db_xref="GeneID:853100"
mRNA complement(<872044..>875109)
/gene="BUB1"
/locus_tag="YGR188C"
/product="protein kinase BUB1"
/transcript_id="NM_001181317.3"
/db_xref="GeneID:853100"
CDS complement(872044..875109)
/gene="BUB1"
/locus_tag="YGR188C"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0000776 kinetochore
[PMID:12769845|PMID:27170178|PMID:24402315|PMID:12221113]"
/experiment="EXISTENCE:direct assay:GO:0000779 condensed
chromosome, centromeric region [PMID:16541024]"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:7969164]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:7969164]"
/experiment="EXISTENCE:mutant phenotype:GO:0007094 mitotic
spindle assembly checkpoint signaling [PMID:1651171]"
/experiment="EXISTENCE:mutant phenotype:GO:0016236
macroautophagy [PMID:19793921]"
/experiment="EXISTENCE:mutant phenotype:GO:0031134 sister
chromatid biorientation [PMID:18081426]"
/experiment="EXISTENCE:mutant phenotype:GO:0034501 protein
localization to kinetochore [PMID:16357219|PMID:24402315]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:12769845]"
/note="Protein kinase required for the spindle assembly
checkpoint; prevents anaphase onset in the presence of
spindle damage; Cdc28p-mediated phosphorylation (T566) is
essential for anaphase degradation and checkpoint
adaptation; associates with CEN DNA via Skp1p; relocalizes
Sgo1p in response to sister kinetochore tension; BUB1 has
a highly diverged paralog, MAD3, that arose from the whole
genome duplication; N-terminal (Nt) TPR domain-containing
region is homologous with the Nt region of Mad3p"
/codon_start=1
/product="protein kinase BUB1"
/protein_id="NP_011704.3"
/db_xref="GeneID:853100"
/db_xref="SGD:S000003420"
/translation="MNLDLGSTVRGYESDKDTFPQSKGVSSSQKEQHSQLNQTKIAYE
QRLLNDLEDMDDPLDLFLDYMIWISTSYIEVDSESGQEVLRSTMERCLIYIQDMETYR
NDPRFLKIWIWYINLFLSNNFHESENTFKYMFNKGIGTKLSLFYEEFSKLLENAQFFL
EAKVLLELGAENNCRPYNRLLRSLSNYEDRLREMNIVENQNSVPDSRERLKGRLIYRT
APFFIRKFLTSSLMTDDKENRANLNSNVGVGKSAPNVYQDSIVVADFKSETERLNLNS
SKQPSNQRLKNGNKKTSIYADQKQSNNPVYKLINTPGRKPERIVFNFNLIYPENDEEF
NTEEILAMIKGLYKVQRRGKKHTEDYTSDKNRKKRKLDVLVERRQDLPSSQPPVVPKS
TRIEVFKDDDNPSQSTHHKNTQVQVQTTTSILPLKPVVDGNLAHETPVKPSLTSNASR
SPTVTAFSKDAINEVFSMFNQHYSTPGALLDGDDTTTSKFNVFENFTQEFTAKNIEDL
TEVKDPKQETVSQQTTSTNETNDRYERLSNSSTRPEKADYMTPIKETTETDVVPIIQT
PKEQIRTEDKKSGDNTETQTQLTSTTIQSSPFLTQPEPQAEKLLQTAEHSEKSKEHYP
TIIPPFTKIKNQPPVIIENPLSNNLRAKFLSEISPPLFQYNTFYNYNQELKMSSLLKK
IHRVSRNENKNPIVDFKKTGDLYCIRGELGEGGYATVYLAESSQGHLRALKVEKPASV
WEYYIMSQVEFRLRKSTILKSIINASALHLFLDESYLVLNYASQGTVLDLINLQREKA
IDGNGIMDEYLCMFITVELMKVLEKIHEVGIIHGDLKPDNCMIRLEKPGEPLGAHYMR
NGEDGWENKGIYLIDFGRSFDMTLLPPGTKFKSNWKADQQDCWEMRAGKPWSYEADYY
GLAGVIHSMLFGKFIETIQLQNGRCKLKNPFKRYWKKEIWGVIFDLLLNSGQASNQAL
PMTEKIVEIRNLIESHLEQHAENHLRNVILSIEEELSHFQYKGKPSRRF"
repeat_region 875951..876279
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006976"
gene 876394..876466
/locus_tag="YNCG0041W"
/db_xref="GeneID:853101"
tRNA 876394..876466
/locus_tag="YNCG0041W"
/product="tRNA-Lys"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:8915539]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:8915539]"
/experiment="EXISTENCE:mutant phenotype:GO:0030533 triplet
codon-amino acid adaptor activity [PMID:17560369]"
/experiment="EXISTENCE:mutant phenotype:GO:0032543
mitochondrial translation [PMID:17560369]"
/note="Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE
analysis; a small portion is imported into mitochondria
via interaction with mt lysyl-tRNA synthetase Msk1p and is
necessary to decode AAG codons at high temperature, when
base modification of mt-encoded tRNA-Lys is reduced"
/db_xref="GeneID:853101"
/db_xref="SGD:S000006623"
gene complement(<876669..>878192)
/gene="CRH1"
/locus_tag="YGR189C"
/db_xref="GeneID:853102"
mRNA complement(<876669..>878192)
/gene="CRH1"
/locus_tag="YGR189C"
/product="transglycosylase"
/transcript_id="NM_001181318.1"
/db_xref="GeneID:853102"
CDS complement(876669..878192)
/gene="CRH1"
/locus_tag="YGR189C"
/EC_number="3.2.1.14"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:10757808]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:15781460|PMID:10757808]"
/experiment="EXISTENCE:direct assay:GO:0016757
glycosyltransferase activity [PMID:23919454]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0006030
chitin metabolic process [PMID:18694928]"
/experiment="EXISTENCE:genetic interaction:GO:0016757
glycosyltransferase activity [PMID:18694928]"
/experiment="EXISTENCE:genetic interaction:GO:0031505
fungal-type cell wall organization [PMID:18694928]"
/experiment="EXISTENCE:mutant phenotype:GO:0006030 chitin
metabolic process [PMID:18694928]"
/experiment="EXISTENCE:mutant phenotype:GO:0016757
glycosyltransferase activity [PMID:18694928]"
/experiment="EXISTENCE:mutant phenotype:GO:0031505
fungal-type cell wall organization [PMID:18694928]"
/note="Chitin transglycosylase; functions in the transfer
of chitin to beta(1-6) and beta(1-3) glucans in the cell
wall; similar and functionally redundant to Utr2;
localizes to sites of polarized growth; expression induced
by cell wall stress"
/codon_start=1
/product="transglycosylase"
/protein_id="NP_011705.1"
/db_xref="GeneID:853102"
/db_xref="SGD:S000003421"
/translation="MKVLDLLTVLSASSLLSTFAAAESTATADSTTAASSTASCNPLK
TTGCTPDTALATSFSEDFSSSSKWFTDLKHAGEIKYGSDGLSMTLAKRYDNPSLKSNF
YIMYGKLEVILKAANGTGIVSSFYLQSDDLDEIDIEWVGGDNTQFQSNFFSKGDTTTY
DRGEFHGVDTPTDKFHNYTLDWAMDKTTWYLDGESVRVLSNTSSEGYPQSPMYLMMGI
WAGGDPDNAAGTIEWAGGETNYNDAPFTMYIEKVIVTDYSTGKKYTYGDQSGSWESIE
ADGGSIYGRYDQAQEDFAVLANGGSISSSSTSSSTVSSSASSTVSSSVSSTVSSSASS
TVSSSVSSTVSSSSSVSSSSSTSPSSSTATSSKTLASSSVTTSSSISSFEKQSSSSSK
KTVASSSTSESIISSTKTPATVSSTTRSTVAPTTQQSSVSSDSPVQDKGGVATSSNDV
TSSTTQISSKYTSTIQSSSSEASSTNSVQISNGADLAQSLPREGKLFSVLVALLALL"
gene complement(878710..878815)
/locus_tag="YNCG0042C"
/db_xref="GeneID:853103"
tRNA complement(join(878710..878745,878780..878815))
/locus_tag="YNCG0042C"
/product="tRNA-Trp"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Tryptophan tRNA (tRNA-Trp), predicted by
tRNAscan-SE analysis"
/db_xref="GeneID:853103"
/db_xref="SGD:S000006772"
gene <880420..>882231
/gene="HIP1"
/locus_tag="YGR191W"
/db_xref="GeneID:853104"
mRNA <880420..>882231
/gene="HIP1"
/locus_tag="YGR191W"
/product="histidine permease"
/transcript_id="NM_001181320.1"
/db_xref="GeneID:853104"
CDS 880420..882231
/gene="HIP1"
/locus_tag="YGR191W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0005291
high-affinity L-histidine transmembrane transporter
activity [PMID:5447432]"
/experiment="EXISTENCE:mutant phenotype:GO:0005886 plasma
membrane [PMID:3905514]"
/experiment="EXISTENCE:mutant phenotype:GO:0006828
manganese ion transport [PMID:9790586]"
/experiment="EXISTENCE:mutant phenotype:GO:0015817
histidine transport [PMID:3905514|PMID:5447432]"
/note="High-affinity histidine permease; also involved in
the transport of manganese ions"
/codon_start=1
/product="histidine permease"
/protein_id="NP_011707.1"
/db_xref="GeneID:853104"
/db_xref="SGD:S000003423"
/translation="MPRNPLKKEYWADVVDGFKPATSPAFENEKESTTFVTELTSKTD
SAFPLSSKDSPGINQTTNDITSSDRFRRNEDTEQEDINNTNLSKDLSVRHLLTLAVGG
AIGTGLYVNTGAALSTGGPASLVIDWVIISTCLFTVINSLGELSAAFPVVGGFNVYSM
RFIEPSFAFAVNLNYLAQWLVLLPLELVAASITIKYWNDKINSDAWVAIFYATIALAN
MLDVKSFGETEFVLSMIKILSIIGFTILGIVLSCGGGPHGGYIGGKYWHDPGAFVGHS
SGTQFKGLCSVFVTAAFSYSGIEMTAVSAAESKNPRETIPKAAKRTFWLITASYVTIL
TLIGCLVPSNDPRLLNGSSSVDAASSPLVIAIENGGIKGLPSLMNAIILIAVVSVANS
AVYACSRCMVAMAHIGNLPKFLNRVDKRGRPMNAILLTLFFGLLSFVAASDKQAEVFT
WLSALSGLSTIFCWMAINLSHIRFRQAMKVQERSLDELPFISQTGVKGSWYGFIVLFL
VLIASFWTSLFPLGGSGASAESFFEGYLSFPILIVCYVGHKLYTRNWTLMVKLEDMDL
DTGRKQVDLTLRREEMRIERETLAKRSFVTRFLHFWC"
gene complement(<882812..>883810)
/gene="TDH3"
/locus_tag="YGR192C"
/gene_synonym="GLD1; HSP35; HSP36; SSS2"
/db_xref="GeneID:853106"
mRNA complement(<882812..>883810)
/gene="TDH3"
/locus_tag="YGR192C"
/gene_synonym="GLD1; HSP35; HSP36; SSS2"
/product="glyceraldehyde-3-phosphate dehydrogenase
(phosphorylating) TDH3"
/transcript_id="NM_001181321.3"
/db_xref="GeneID:853106"
CDS complement(882812..883810)
/gene="TDH3"
/locus_tag="YGR192C"
/gene_synonym="GLD1; HSP35; HSP36; SSS2"
/EC_number="1.2.1.12"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:25288528]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:18959479|PMID:37792491]"
/experiment="EXISTENCE:direct assay:GO:0004365
glyceraldehyde-3-phosphate dehydrogenase (NAD+)
(phosphorylating) activity [PMID:3905788]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16962558|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005811 lipid
droplet [PMID:10515935]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:11158358|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:11158358]"
/experiment="EXISTENCE:direct assay:GO:1904408 melatonin
binding [PMID:31708896]"
/experiment="EXISTENCE:expression pattern:GO:0006094
gluconeogenesis [PMID:3905788]"
/experiment="EXISTENCE:expression pattern:GO:0006096
glycolytic process [PMID:3905788]"
/experiment="EXISTENCE:mutant phenotype:GO:0006915
apoptotic process [PMID:17726063]"
/experiment="EXISTENCE:mutant phenotype:GO:0015886 heme
transport [PMID:27247412]"
/experiment="EXISTENCE:mutant phenotype:GO:0020037 heme
binding [PMID:30012884]"
/experiment="EXISTENCE:mutant phenotype:GO:0072593
reactive oxygen species metabolic process [PMID:17726063]"
/note="Glyceraldehyde-3-phosphate dehydrogenase (GAPDH),
isozyme 3; involved in glycolysis and gluconeogenesis;
tetramer that catalyzes the reaction of
glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate;
detected in the cytoplasm and cell wall; GAPDH-derived
antimicrobial peptides secreted by S. cerevisiae are
active against a wide variety of wine-related yeasts and
bacteria; moonlighting role as an AU-rich mRNA binding
protein that binds to the FAS1 mRNA and controls periodic
abundance of Fas1p"
/codon_start=1
/product="glyceraldehyde-3-phosphate dehydrogenase
(phosphorylating) TDH3"
/protein_id="NP_011708.3"
/db_xref="GeneID:853106"
/db_xref="SGD:S000003424"
/translation="MVRVAINGFGRIGRLVMRIALSRPNVEVVALNDPFITNDYAAYM
FKYDSTHGRYAGEVSHDDKHIIVDGKKIATYQERDPANLPWGSSNVDIAIDSTGVFKE
LDTAQKHIDAGAKKVVITAPSSTAPMFVMGVNEEKYTSDLKIVSNASCTTNCLAPLAK
VINDAFGIEEGLMTTVHSLTATQKTVDGPSHKDWRGGRTASGNIIPSSTGAAKAVGKV
LPELQGKLTGMAFRVPTVDVSVVDLTVKLNKETTYDEIKKVVKAAAEGKLKGVLGYTE
DAVVSSDFLGDSHSSIFDASAGIQLSPKFVKLVSWYDNEYGYSTRVVDLVEHVAKA"
gene complement(<884509..>885741)
/gene="PDX1"
/locus_tag="YGR193C"
/db_xref="GeneID:853107"
mRNA complement(<884509..>885741)
/gene="PDX1"
/locus_tag="YGR193C"
/product="Pdx1p"
/transcript_id="NM_001181322.1"
/db_xref="GeneID:853107"
CDS complement(884509..885741)
/gene="PDX1"
/locus_tag="YGR193C"
/experiment="EXISTENCE:direct assay:GO:0005198 structural
molecule activity [PMID:2007123]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0006086 pyruvate
decarboxylation to acetyl-CoA [PMID:7947791|PMID:2007123]"
/experiment="EXISTENCE:direct assay:GO:0045254 pyruvate
dehydrogenase complex [PMID:2007123|PMID:7947791]"
/experiment="EXISTENCE:mutant phenotype:GO:0005198
structural molecule activity [PMID:2007123]"
/experiment="EXISTENCE:mutant phenotype:GO:0006086
pyruvate decarboxylation to acetyl-CoA [PMID:2007123]"
/experiment="EXISTENCE:physical interaction:GO:0045254
pyruvate dehydrogenase complex [PMID:7947791]"
/note="E3-binding protein of the mitochondrial pyruvate
dehydrogenase complex; plays a structural role in the
complex by binding and positioning dihydrolipoamide
dehydrogenase (E3) to the dihydrolipoamide
acetyltransferase (E2) core"
/codon_start=1
/product="Pdx1p"
/protein_id="NP_011709.1"
/db_xref="GeneID:853107"
/db_xref="SGD:S000003425"
/translation="MLSAISKVSTLKSCTRYLTKCNYHASAKLLAVKTFSMPAMSPTM
EKGGIVSWKYKVGEPFSAGDVILEVETDKSQIDVEALDDGKLAKILKDEGSKDVDVGE
PIAYIADVDDDLATIKLPQEANTANAKSIEIKKPSADSTEATQQHLKKATVTPIKTVD
GSQANLEQTLLPSVSLLLAENNISKQKALKEIAPSGSNGRLLKGDVLAYLGKIPQDSV
NKVTEFIKKNERLDLSNIKPIQLKPKIAEQAQTKAADKPKITPVEFEEQLVFHAPASI
PFDKLSESLNSFMKEAYQFSHGTPLMDTNSKYFDPIFEDLVTLSPREPRFKFSYDLMQ
IPKANNMQDTYGQEDIFDLLTGSDATASSVRPVEKNLPEKNEYILALNVSVNNKKFND
AEAKAKRFLDYVRELESF"
gene complement(<886073..>887875)
/gene="XKS1"
/locus_tag="YGR194C"
/db_xref="GeneID:853108"
mRNA complement(<886073..>887875)
/gene="XKS1"
/locus_tag="YGR194C"
/product="xylulokinase"
/transcript_id="NM_001181323.3"
/db_xref="GeneID:853108"
CDS complement(886073..887875)
/gene="XKS1"
/locus_tag="YGR194C"
/EC_number="2.7.1.17"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005998 xylulose
catabolic process [PMID:10981687]"
/experiment="EXISTENCE:mutant phenotype:GO:0004856
D-xylulokinase activity [PMID:9595677]"
/note="Xylulokinase; converts D-xylulose and ATP to
xylulose 5-phosphate and ADP; rate limiting step in
fermentation of xylulose; required for xylose fermentation
by recombinant S. cerevisiae strains"
/codon_start=1
/product="xylulokinase"
/protein_id="NP_011710.3"
/db_xref="GeneID:853108"
/db_xref="SGD:S000003426"
/translation="MLCSVIQRQTREVSNTMSLDSYYLGFDLSTQQLKCLAINQDLKI
VHSETVEFEKDLPHYHTKKGVYIHGDTIECPVAMWLEALDLVLSKYREAKFPLNKVMA
VSGSCQQHGSVYWSSQAESLLEQLNKKPEKDLLHYVSSVAFARQTAPNWQDHSTAKQC
QEFEECIGGPEKMAQLTGSRAHFRFTGPQILKIAQLEPEAYEKTKTISLVSNFLTSIL
VGHLVELEEADACGMNLYDIRERKFSDELLHLIDSSSKDKTIRQKLMRAPMKNLIAGT
ICKYFIEKYGFNTNCKVSPMTGDNLATICSLPLRKNDVLVSLGTSTTVLLVTDKYHPS
PNYHLFIHPTLPNHYMGMICYCNGSLARERIRDELNKERENNYEKTNDWTLFNQAVLD
DSESSENELGVYFPLGEIVPSVKAINKRVIFNPKTGMIEREVAKFKDKRHDAKNIVES
QALSCRVRISPLLSDSNASSQQRLNEDTIVKFDYDESPLRDYLNKRPERTFFVGGASK
NDAIVKKFAQVIGATKGNFRLETPNSCALGGCYKAMWSLLYDSNKIAVPFDKFLNDNF
PWHVMESISDVDNENWDRYNSKIVPLSELEKTLI"
rep_origin 888413..888493
/note="ARS737; Autonomously Replicating Sequence"
/db_xref="SGD:S000119032"
gene <888882..>889622
/gene="SKI6"
/locus_tag="YGR195W"
/gene_synonym="ECM20; RRP41"
/db_xref="GeneID:853109"
mRNA <888882..>889622
/gene="SKI6"
/locus_tag="YGR195W"
/gene_synonym="ECM20; RRP41"
/product="exosome non-catalytic core subunit SKI6"
/transcript_id="NM_001181324.3"
/db_xref="GeneID:853109"
CDS 888882..889622
/gene="SKI6"
/locus_tag="YGR195W"
/gene_synonym="ECM20; RRP41"
/experiment="EXISTENCE:direct assay:GO:0000176 nuclear
exosome (RNase complex) [PMID:10465791|PMID:19046973]"
/experiment="EXISTENCE:direct assay:GO:0000177 cytoplasmic
exosome (RNase complex) [PMID:19046973|PMID:10465791]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:32554806]"
/experiment="EXISTENCE:direct assay:GO:0071038
TRAMP-dependent tRNA surveillance pathway
[PMID:17643380|PMID:15828860]"
/experiment="EXISTENCE:genetic interaction:GO:0034473 U1
snRNA 3'-end processing [PMID:10508172]"
/experiment="EXISTENCE:genetic interaction:GO:0034475 U4
snRNA 3'-end processing [PMID:10508172]"
/experiment="EXISTENCE:genetic interaction:GO:0034476 U5
snRNA 3'-end processing [PMID:10508172]"
/experiment="EXISTENCE:genetic interaction:GO:0070478
nuclear-transcribed mRNA catabolic process, 3'-5'
exonucleolytic nonsense-mediated decay
[PMID:12769863|PMID:12881429]"
/experiment="EXISTENCE:genetic interaction:GO:0071028
nuclear mRNA surveillance [PMID:11030620]"
/experiment="EXISTENCE:mutant phenotype:GO:0000467
exonucleolytic trimming to generate mature 3'-end of 5.8S
rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S
rRNA, LSU-rRNA) [PMID:10508172|PMID:9390555]"
/experiment="EXISTENCE:mutant phenotype:GO:0000956
nuclear-transcribed mRNA catabolic process [PMID:9482746]"
/experiment="EXISTENCE:mutant phenotype:GO:0016075 rRNA
catabolic process [PMID:10508172]"
/experiment="EXISTENCE:mutant phenotype:GO:0034473 U1
snRNA 3'-end processing [PMID:10508172]"
/experiment="EXISTENCE:mutant phenotype:GO:0034475 U4
snRNA 3'-end processing [PMID:10611222|PMID:10508172]"
/experiment="EXISTENCE:mutant phenotype:GO:0034476 U5
snRNA 3'-end processing [PMID:10508172]"
/experiment="EXISTENCE:mutant phenotype:GO:0070478
nuclear-transcribed mRNA catabolic process, 3'-5'
exonucleolytic nonsense-mediated decay [PMID:12881429]"
/experiment="EXISTENCE:mutant phenotype:GO:0071028 nuclear
mRNA surveillance [PMID:11030620]"
/experiment="EXISTENCE:mutant phenotype:GO:0071039 nuclear
polyadenylation-dependent CUT catabolic process
[PMID:18591258]"
/experiment="EXISTENCE:mutant phenotype:GO:0071051
poly(A)-dependent snoRNA 3'-end processing
[PMID:10611222]"
/note="Exosome non-catalytic core component; involved in
3'-5' RNA processing and degradation in both the nucleus
and the cytoplasm; has similarity to E. coli RNase PH and
to human hRrp41p (EXOSC4)"
/codon_start=1
/product="exosome non-catalytic core subunit SKI6"
/protein_id="NP_011711.3"
/db_xref="GeneID:853109"
/db_xref="SGD:S000003427"
/translation="MSRLEIYSPEGLRLDGRRWNELRRFESSINTHPHAADGSSYMEQ
GNNKIITLVKGPKEPRLKSQMDTSKALLNVSVNITKFSKFERSKSSHKNERRVLEIQT
SLVRMFEKNVMLNIYPRTVIDIEIHVLEQDGGIMGSLINGITLALIDAGISMFDYISG
ISVGLYDTTPLLDTNSLEENAMSTVTLGVVGKSEKLSLLLVEDKIPLDRLENVLAIGI
AGAHRVRDLMDEELRKHAQKRVSNASAR"
gene complement(<889733..>892186)
/gene="FYV8"
/locus_tag="YGR196C"
/db_xref="GeneID:853110"
mRNA complement(<889733..>892186)
/gene="FYV8"
/locus_tag="YGR196C"
/product="Fyv8p"
/transcript_id="NM_001181325.1"
/db_xref="GeneID:853110"
CDS complement(889733..892186)
/gene="FYV8"
/locus_tag="YGR196C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/note="hypothetical protein; required for survival upon
exposure to K1 killer toxin"
/codon_start=1
/product="Fyv8p"
/protein_id="NP_011712.1"
/db_xref="GeneID:853110"
/db_xref="SGD:S000003428"
/translation="MSTEQVGRKKSYRWVSASQASYDGAGWDSSDEYDYSSEDGTKGS
EIHKQKISNLPSLPKLNYTDVNGEHDENTGENKDSNDNNVSKSDISPSDKEVGYLSDG
VPKLMASRESVELQAKKSSEHSKSDYLSSTASLKSPSENKKSPHTNRAVNEDLDNLIE
QISREMTPEIRQTSDFRRDSDSCDEIQNEAPLGEAVPSSSSPVEEDEKSHSLGVSMDT
NEADTTFNTPTRNGNEHLSSDGDVSEQKDDEFKVSERGYLADILPAEKEENLQQEDDG
EVESSGALEKKEKSEEKTSIRNRNSTSSGQDKVAKPKPVANETKTSDNGYRNSFFNDY
QHSSDSEEDDNNEGNSGSSDDDNRSSVSDKHADINRQSKQLDTTDDDALSYTESIKYS
TNETEEEDNEDNESIEDKNEDNESIEDENEDTDSYKFSNREKGSILLTSDEEEEEKGM
SSDSDEGSLKAPKSGYFSKMIGNDDKGDSALQPNQIDTIENTNLSNSGSELENSDGSD
EEDHINEDKVLEESSVKDSTDVDSWKPDSEALRSGFVQDTANKKAPPGYVIDSNGKLV
DLTPASMKPRVVSTYSEMESTWDAFPSKGEDDDLETIRDTKTIYDNNTIYNVPGLIGN
QSNLPPLPMDAQEQLNAGNDNSTTDNDNSNNTANDLAARSASFKSENRTVSQGEMTSV
HEPSTEEMAKLGQQNNLPKLDMNKLLNSKTSHAGKIEQLRNYKRELDEYDTGIQTWIN
YTLKSSSNKDKDFIAEEYKQHSHVREAYANADDLSKKHTVINTVASVNQNVTHLRRKV
FQHSMKPKDLFASIGKKKL"
gene complement(<892497..>894140)
/gene="SNG1"
/locus_tag="YGR197C"
/db_xref="GeneID:853111"
mRNA complement(<892497..>894140)
/gene="SNG1"
/locus_tag="YGR197C"
/product="Sng1p"
/transcript_id="NM_001181326.1"
/db_xref="GeneID:853111"
CDS complement(892497..894140)
/gene="SNG1"
/locus_tag="YGR197C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:20424846]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0061091
regulation of phospholipid translocation [PMID:27033517]"
/experiment="EXISTENCE:mutant phenotype:GO:0015931
nucleobase-containing compound transport [PMID:20424846]"
/experiment="EXISTENCE:mutant phenotype:GO:0060237
regulation of fungal-type cell wall organization
[PMID:27033517]"
/note="Protein involved in resistance to nitrosoguanidine
and 6-azauracil; expression is regulated by transcription
factors involved in multidrug resistance; SNG1 has a
paralog, YJR015W, that arose from the whole genome
duplication"
/codon_start=1
/product="Sng1p"
/protein_id="NP_011713.1"
/db_xref="GeneID:853111"
/db_xref="SGD:S000003429"
/translation="MTKSVGDEESQYIEDPSFAAAAAFTGGRDGVSYSNQRFAEGSGH
SSDLAKSLEDYRPPDEKPSSLSSVGEGGANEEEKGGNDGGPLARIQTGLFSPRLRNHR
KKILSKFVLNNFFIACVCVSLISIYWGACYGTDRYFFKVKNIVVLQDAPSNTSVQSIS
AIIPSLLASVPGTWHIYNATSFHRKFGTTNSTEIDRKIVDLIYDERYWLALNVKPNAT
DTLYNSLISQDANSEFNSSIFFESVFESGRDPSSVKSTILPLMQQLEVRLQKYYVKEY
LPSLMSNITSNDRDLNINMENWAIAGQLLFTYNDYRPFADRILMAPLQVGLIYCILLT
VLQLSLYGKLHGEMARVLKPKHILIYRLLISWATYFLLSIGFCTVSAIFRIDFTPAFG
RGGFVVYWMSTWLVMMAVGGANENVLSLVIAYCPPYLSIWLMTWIILNISASFYPMVL
NNEFYRYGYIMPIHNAVDIYKVIFLNLTKRKMGRNYGILVAWVALNTSLMPFCMKFAG
KKMQKNAMQAAEAAVAAATQRASRPAEANTDKNNNPPGN"
gene <894693..>897146
/gene="YPP1"
/locus_tag="YGR198W"
/db_xref="GeneID:853112"
mRNA <894693..>897146
/gene="YPP1"
/locus_tag="YGR198W"
/product="Ypp1p"
/transcript_id="NM_001181327.3"
/db_xref="GeneID:853112"
CDS 894693..897146
/gene="YPP1"
/locus_tag="YGR198W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:17576801]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762|PMID:17576801]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:14690591|PMID:19075114]"
/experiment="EXISTENCE:direct assay:GO:0006623 protein
targeting to vacuole [PMID:17576801]"
/experiment="EXISTENCE:direct assay:GO:0006898
receptor-mediated endocytosis [PMID:17576801]"
/experiment="EXISTENCE:direct assay:GO:0030479 actin
cortical patch [PMID:17576801]"
/experiment="EXISTENCE:direct assay:GO:0072659 protein
localization to plasma membrane [PMID:19075114]"
/experiment="EXISTENCE:mutant phenotype:GO:0006623 protein
targeting to vacuole [PMID:17576801]"
/note="Cargo-transport protein involved in endocytosis;
interacts with phosphatidylinositol-4-kinase Stt4p; is
required, along with Efr3p, for the assembly and
recruitment of multiple copies of the kinase into
phosphoinositide kinase (PIK) patches at the plasma
membrane; positively regulates Stt4p; GFP-fusion protein
localizes to the cytoplasm; YGR198W is an essential gene"
/codon_start=1
/product="Ypp1p"
/protein_id="NP_011714.3"
/db_xref="GeneID:853112"
/db_xref="SGD:S000003430"
/translation="MPNSNVRIPPTVPSKIIDVVDQALRARLLGGSTFNSGFDSLDSV
LNLQFRLHYHVIGSNGPAKPVCDVLLKESQNLEKNMSMMEELNDYPEITKLVEKILFN
CLGILFFHRGQFQESQRCLLHSLKIHNNTASQKTALMEQYDRYLIVENLYYRGLVSQD
INIMQNVFYKELLAHVDTIPPESNGLLFEYISLIVAKLRFNQIQDLAENFKTTVENPF
ILFLYMIKKFQSPLKKHIDNDDLYLKFGQNVLLKAKFPTASETNDEALEHFNVFLQYY
FKFTHIKKIKVNPSWYNFIISSMEKTFQSIEVSKTAMFLFQNLSDNSNDEIKKKTFKR
ESILNFVNFVKYNDKYYQLHDNSHRDIISFIDAYSFILQNSSKTDSIENVFDYDNTVS
TFATSLNSFYKEYNLPLMSQSESLDWLENSTRCVYPGNISKVLTNAWSTLYEIRKYQL
DFLVSNNLTSYLCNAMMLSTKEKDNADVEEQEEGEEEKALRELQFKYSYTLAQQRHIE
TAIKTLESLILSKNPNYYKAWHLLALCRSVQEDKEMSYKIVCSVLEAMNESLQNNTLL
LNDRWQFIHLKLTQLALIEEIFGTLEALETLPEVFELYATLFPDSQPELNSMGPKYSQ
TKEYLLQMVWIFAANMYMRTKDNDEDAKAAIKEASNVESKFKNLNCNIANGYLSIIKD
EPGVALKEFETVLYYDENNLDALVGFAELIFPEELGVEETNLERYYTLSLDKKPGKRA
KLTFVNDTDRSAAYARLKFLLECAILESIEAYYSPEVWWYLSLIYEKYQDDEYKNSLL
KCIKYQELNPIRSLRYCNY"
gene <897502..>899781
/gene="PMT6"
/locus_tag="YGR199W"
/db_xref="GeneID:853113"
mRNA <897502..>899781
/gene="PMT6"
/locus_tag="YGR199W"
/product="dolichyl-phosphate-mannose-protein
mannosyltransferase PMT6"
/transcript_id="NM_001181328.1"
/db_xref="GeneID:853113"
CDS 897502..899781
/gene="PMT6"
/locus_tag="YGR199W"
/EC_number="2.4.1.109"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0006493 protein
O-linked glycosylation [PMID:9184828]"
/experiment="EXISTENCE:mutant phenotype:GO:0035269 protein
O-linked glycosylation via mannose [PMID:18182384]"
/note="Protein O-mannosyltransferase; transfers mannose
from dolichyl phosphate-D-mannose to protein
serine/threonine residues of secretory proteins; reaction
is essential for cell wall rigidity; member of a family of
mannosyltransferases"
/codon_start=1
/product="dolichyl-phosphate-mannose-protein
mannosyltransferase PMT6"
/protein_id="NP_011715.1"
/db_xref="GeneID:853113"
/db_xref="SGD:S000003431"
/translation="MSKAKGTGFSSIDTEDENLRERYVNQPKANASDIQDEQLDCFEQ
LEEKHRTKKNEEYTALKILRDVIGPLLLTITSFYLRFQHIDQNNYVVWDEAHFGKFGS
YYIKHEYYHDVHPPLGKMLIALSEWMAGFDGQFDFSSNNAYPENVNFKLMRQFNATFG
ALCTPVAFFTAKWMGFNYFTVYLIATMVTLEHSYIVLSKFILLDSMLLFFSMTTFACM
IKLYTLRKQQMTKKWSLWMLLTGLSIGCVCSVKWVGLFITVVVGLYTCIELFLLYCDK
ELPRIKYYKHWLIRIINLIVIPFLIYLYCFKIHFVLLYKSGTGDSTTNTLFQINLEGT
QIEAGPRDVAFGSELTIRSHGLSPNLLHSHIQVYPEGSGQRQITGYGFADSNNVWKFE
FSRSSGLELDQNGTLNGKIIPITDGVEVRLSHKNTGSNLHSHDVPSHVSRGNYEVSGY
GSQSVGDEKDDWIVEIVKQMDSPNPVYSNENSTILHPVSTFFRLRHKVLGCYLASTGL
TYPAWGFKQAEIVCKDSWSRRDKSTWWNVEDHWNHNLETAEDYVPPKSNFWTDFILTN
FAMASSNNALVPDEDKYDSLSSDAWEWPTLHKGLRMCSWAGYITRYYLMGSPFNTWIS
TVSLIIFPFIILFILYRWRRQTLYLSDDQIWQITIQGIFPFISWMTHYLPFAMMGRVT
YVHHYVPALYFAMLVFGFVLDFTLTRVHWMVKYPIYLSLFGGCIYIYNLFAPICQGMH
GDKAEYLPLQWLSTWDIAP"
gene complement(<899904..>902270)
/gene="ELP2"
/locus_tag="YGR200C"
/gene_synonym="KTI3; TOT2"
/db_xref="GeneID:853114"
mRNA complement(<899904..>902270)
/gene="ELP2"
/locus_tag="YGR200C"
/gene_synonym="KTI3; TOT2"
/product="Elongator subunit ELP2"
/transcript_id="NM_001181329.3"
/db_xref="GeneID:853114"
CDS complement(899904..902270)
/gene="ELP2"
/locus_tag="YGR200C"
/gene_synonym="KTI3; TOT2"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12139626]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:12139626]"
/experiment="EXISTENCE:direct assay:GO:0008017 microtubule
binding [PMID:25960406]"
/experiment="EXISTENCE:direct assay:GO:0033588 elongator
holoenzyme complex [PMID:11689709]"
/experiment="EXISTENCE:mutant phenotype:GO:0002098 tRNA
wobble uridine modification [PMID:18755837]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:11689709]"
/experiment="EXISTENCE:mutant phenotype:GO:0032447 protein
urmylation [PMID:14551258]"
/note="Subunit of Elongator complex; binds to microtubules
via conserved alkaline residues; has two seven-bladed WD40
beta propellers; Elongator complex is required for
modification of wobble nucleosides in tRNA; target of
Kluyveromyces lactis zymocin"
/codon_start=1
/product="Elongator subunit ELP2"
/protein_id="NP_011716.3"
/db_xref="GeneID:853114"
/db_xref="SGD:S000003432"
/translation="MVECITPEAIFIGANKQTQVSDIHKVKKIVAFGAGKTIALWDPI
EPNNKGVYATLKGHEAEVTCVRFVPDSDFMVSASEDHHVKIWKFTDYSHLQCIQTIQH
YSKTIVALSALPSLISVGCADGTISIWRQNIQNDEFGLAHEFTIKKGFFYPLCLSLSK
VEEKKYLLAIGGTNVNVFIASFILSDSGIEKCRVVAELEGHEDWVKSLAFRHQETPGD
YLLCSGSQDRYIRLWRIRINDLIDDSEEDSKKLTLLSNKQYKFQIDDELRVGINFEAL
IMGHDDWISSLQWHESRLQLLAATADTSLMVWEPDETSGIWVCSLRLGEMSSKGASTA
TGSSGGFWSCLWFTHERMDFFLTNGKTGSWRMWATKDNIICDQRLGISGATKDVTDIA
WSPSGEYLLATSLDQTTRLFAPWIYDASGRKREIATWHEFSRPQIHGYDMICVETVTD
TRFVSGGDEKILRSFDLPKGVAGMLQKFVGIQFEEKSEMPDSATVPVLGLSNKAGEDD
ANEDDEEEEGGNKETPDITDPLSLLECPPMEDQLQRHLLWPEVEKLYGHGFEITCLDI
SPDQKLIASACRSNNVQNAVIRIFSTENWLEIKPALPFHSLTITRLKFSKDGKFLLSV
CRDRKWALWERNMEDNTFELRFKNEKPHTRIIWDADWAPLEFGNVFVTASRDKTVKVW
RHQKEPADDYVLEASIKHTKAVTAISIHDSMIREKILISVGLENGEIYLYSYTLGKFE
LITQLNEDITPADKITRLRWSHLKRNGKLFLGVGSSDLSTRIYSLAYE"
gene complement(<902520..>903197)
/locus_tag="YGR201C"
/db_xref="GeneID:853115"
mRNA complement(<902520..>903197)
/locus_tag="YGR201C"
/product="uncharacterized protein"
/transcript_id="NM_001181330.3"
/db_xref="GeneID:853115"
CDS complement(902520..903197)
/locus_tag="YGR201C"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011717.4"
/db_xref="GeneID:853115"
/db_xref="SGD:S000003433"
/translation="MSDGTLFTDLKERKLIRTIVPRGLVRSLKLDVKLADPSDAQQLY
EREFPLRKYPTFVGPHDEWTLTEAMAIDYYLIHLSSDKEAVRQLLGPEGDFKTRADIL
RWESLSNSDFLNEVCEVFFPLIGVKPYNATEFKAARENVDTIVSLYEKRLKKQQYLVC
DDHETLADLISAAAFSLGFISFFDETWRSKHPEVTRWFNRVIKSRFFEGEFESFKMCE
TEMQPIK"
gene complement(<903474..>904748)
/gene="PCT1"
/locus_tag="YGR202C"
/gene_synonym="BSR2; CCT1"
/db_xref="GeneID:853116"
mRNA complement(<903474..>904748)
/gene="PCT1"
/locus_tag="YGR202C"
/gene_synonym="BSR2; CCT1"
/product="choline-phosphate cytidylyltransferase"
/transcript_id="NM_001181331.1"
/db_xref="GeneID:853116"
CDS complement(903474..904748)
/gene="PCT1"
/locus_tag="YGR202C"
/gene_synonym="BSR2; CCT1"
/EC_number="2.7.7.15"
/experiment="EXISTENCE:direct assay:GO:0004105
choline-phosphate cytidylyltransferase activity
[PMID:2826147]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12200438]"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:12200438]"
/experiment="EXISTENCE:direct assay:GO:0006656
phosphatidylcholine biosynthetic process [PMID:10397762]"
/experiment="EXISTENCE:direct assay:GO:0006657 CDP-choline
pathway [PMID:10397762]"
/experiment="EXISTENCE:direct assay:GO:0042564
NLS-dependent protein nuclear import complex
[PMID:19141610]"
/note="Cholinephosphate cytidylyltransferase; a
rate-determining enzyme of the CDP-choline pathway for
phosphatidylcholine synthesis, inhibited by Sec14p,
activated upon lipid-binding; controls phosphatidylcholine
homeostasis; contains an element within the regulatory
domain involved in both silencing and activation of
enzymatic activity"
/codon_start=1
/product="choline-phosphate cytidylyltransferase"
/protein_id="NP_011718.1"
/db_xref="GeneID:853116"
/db_xref="SGD:S000003434"
/translation="MANPTTGKSSIRAKLSNSSLSNLFKKNKNKRQREETEEQDNEDK
DESKNQDENKDTQLTPRKRRRLTKEFEEKEARYTNELPKELRKYRPKGFRFNLPPTDR
PIRIYADGVFDLFHLGHMKQLEQCKKAFPNVTLIVGVPSDKITHKLKGLTVLTDKQRC
ETLTHCRWVDEVVPNAPWCVTPEFLLEHKIDYVAHDDIPYVSADSDDIYKPIKEMGKF
LTTQRTNGVSTSDIITKIIRDYDKYLMRNFARGATRQELNVSWLKKNELEFKKHINEF
RSYFKKNQTNLNNASRDLYFEVREILLKKTLGKKLYSKLIGNELKKQNQRQRKQNFLD
DPFTRKLIREASPATEFANEFTGENSTAKSPDDNGNLFSQEDDEDTNSNNTNTNSDSD
SNTNSTPPSEDDDDNDRLTLENLTQKKKQSAN"
gene <905237..>905683
/gene="YCH1"
/locus_tag="YGR203W"
/db_xref="GeneID:853117"
mRNA <905237..>905683
/gene="YCH1"
/locus_tag="YGR203W"
/product="phosphatase YCH1"
/transcript_id="NM_001181332.1"
/db_xref="GeneID:853117"
CDS 905237..905683
/gene="YCH1"
/locus_tag="YGR203W"
/experiment="EXISTENCE:direct assay:GO:0004792
thiosulfate-cyanide sulfurtransferase activity
[PMID:19864628]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0016311
dephosphorylation [PMID:19382206]"
/experiment="EXISTENCE:direct assay:GO:0016791 phosphatase
activity [PMID:19382206]"
/note="Phosphatase with sequence similarity to Mih1p;
member of the Cdc25p subfamily of tyrosine phosphatases
and Arr2p, an arsenate reductase, based on the presence of
a rhodanese-homology domain; green fluorescent protein
(GFP)-fusion protein localizes to both the cytoplasm and
the nucleus"
/codon_start=1
/product="phosphatase YCH1"
/protein_id="NP_011719.1"
/db_xref="GeneID:853117"
/db_xref="SGD:S000003435"
/translation="MDSYSITNVKYLDPTELHRWMQEGHTTTLREPFQVVDVRGSDYM
GGHIKDGWHYAYSRLKQDPEYLRELKHRLLEKQADGRGALNVIFHCMLSQQRGPSAAM
LLLRSLDTAELSRCRLWVLRGGFSRWQSVYGDDESVTAGYLPDLWR"
gene <905934..>908774
/gene="ADE3"
/locus_tag="YGR204W"
/db_xref="GeneID:853118"
mRNA <905934..>908774
/gene="ADE3"
/locus_tag="YGR204W"
/product="trifunctional formate-tetrahydrofolate
ligase/methenyltetrahydrofolate
cyclohydrolase/methylenetetrahydrofolate dehydrogenase
ADE3"
/transcript_id="NM_001181333.3"
/db_xref="GeneID:853118"
CDS 905934..908774
/gene="ADE3"
/locus_tag="YGR204W"
/EC_number="1.5.1.5"
/EC_number="3.5.4.9"
/EC_number="6.3.4.3"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276|PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0004329
formate-tetrahydrofolate ligase activity [PMID:8464869]"
/experiment="EXISTENCE:mutant phenotype:GO:0004477
methenyltetrahydrofolate cyclohydrolase activity
[PMID:8464869]"
/experiment="EXISTENCE:mutant phenotype:GO:0004488
methylenetetrahydrofolate dehydrogenase (NADP+) activity
[PMID:8464869]"
/experiment="EXISTENCE:mutant phenotype:GO:0006760 folic
acid-containing compound metabolic process [PMID:5563870]"
/experiment="EXISTENCE:mutant phenotype:GO:0009113 purine
nucleobase biosynthetic process [PMID:8464869]"
/note="Cytoplasmic trifunctional enzyme; catalyzes
sequential reactions 10-formyl-THF synthetase (EC
6.3.4.3), 5,10-methenyl-THF cyclohydrolase (EC 3.5.4.9),
and 5,10-methylene-THF dehydrogenase (EC 1.5.1.5), to
supply forms of activated one-carbon units required for
biosynthesis of purines, histidine, methionine,
pantothenic acid, the formyl group of mitochondrial
fMet-tRNAfMet, and the methyl group of thymidylate; null
mutation causes auxotrophy for adenine and histidine in
rich medium"
/codon_start=1
/product="trifunctional formate-tetrahydrofolate
ligase/methenyltetrahydrofolate
cyclohydrolase/methylenetetrahydrofolate dehydrogenase
ADE3"
/protein_id="NP_011720.3"
/db_xref="GeneID:853118"
/db_xref="SGD:S000003436"
/translation="MAGQVLDGKACAQQFRSNIANEIKSIQGHVPGFAPNLAIIQVGN
RPDSATYVRMKRKAAEEAGIVANFIHLDESATEFEVLRYVDQLNEDPHTHGIIVQLPL
PAHLDEDRITSRVLAEKDVDGFGPTNIGELNKKNGHPFFLPCTPKGIIELLHKANVTI
EGSRSVVIGRSDIVGSPVAELLKSLNSTVTITHSKTRDIASYLHDADIVVVAIGQPEF
VKGEWFKPRDGTSSDKKTVVIDVGTNYVADPSKKSGFKCVGDVEFNEAIKYVHLITPV
PGGVGPMTVAMLMQNTLIAAKRQMEESSKPLQIPPLPLKLLTPVPSDIDISRAQQPKL
INQLAQELGIYSHELELYGHYKAKISPKVIERLQTRQNGKYILVSGITPTPLGEGKST
TTMGLVQALTAHLGKPAIANVRQPSLGPTLGVKGGAAGGGYSQVIPMDEFNLHLTGDI
HAIGAANNLLAAAIDTRMFHETTQKNDATFYNRLVPRKNGKRKFTPSMQRRLNRLGIQ
KTNPDDLTPEEINKFARLNIDPDTITIKRVVDINDRMLRQITIGQAPTEKNHTRVTGF
DITVASELMAILALSKDLRDMKERIGRVVVAADVNRSPVTVEDVGCTGALTALLRDAI
KPNLMQTLEGTPVLVHAGPFANISIGASSVIADRVALKLVGTEPEAKTEAGYVVTEAG
FDFTMGGERFFNIKCRSSGLTPNAVVLVATVRALKSHGGAPDVKPGQPLPSAYTEENI
EFVEKGAANMCKQIANIKQFGVPVVVAINKFETDTEGEIAAIRKAALEAGAFEAVTSN
HWAEGGKGAIDLAKAVIEASNQPVDFHFLYDVNSSVEDKLTTIVQKMYGGAAIDILPE
AQRKIDMYKEQGFGNLPICIAKTQYSLSHDATLKGVPTGFTFPIRDVRLSNGAGYLYA
LAAEIQTIPGLATYAGYMAVEVDDDGEIDGLF"
gene complement(<909061..>909174)
/locus_tag="YGR204C-A"
/db_xref="GeneID:1466462"
mRNA complement(<909061..>909174)
/locus_tag="YGR204C-A"
/product="uncharacterized protein"
/transcript_id="NM_001184596.1"
/db_xref="GeneID:1466462"
CDS complement(909061..909174)
/locus_tag="YGR204C-A"
/note="hypothetical protein; identified by gene-trapping,
microarray-based expression analysis, and genome-wide
homology searching"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878082.1"
/db_xref="GeneID:1466462"
/db_xref="SGD:S000028640"
/translation="MQWNAFSFVSYVYLRYFISFRPNIVLASVRLSWYSII"
gene <909213..>910085
/gene="TDA10"
/locus_tag="YGR205W"
/db_xref="GeneID:853119"
mRNA <909213..>910085
/gene="TDA10"
/locus_tag="YGR205W"
/product="putative ATP-dependent kinase"
/transcript_id="NM_001181334.3"
/db_xref="GeneID:853119"
CDS 909213..910085
/gene="TDA10"
/locus_tag="YGR205W"
/experiment="EXISTENCE:direct assay:GO:0005524 ATP binding
[PMID:14997573]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276|PMID:14562095]"
/note="ATP-binding hypothetical protein; crystal structure
resembles that of E.coli pantothenate kinase and other
small kinases; null mutant is sensitive to expression of
the top1-T722A allele"
/codon_start=1
/product="putative ATP-dependent kinase"
/protein_id="NP_011721.3"
/db_xref="GeneID:853119"
/db_xref="SGD:S000003437"
/translation="MCDKSKTVLDYTIEFLDKYIPEWFETGNKCPLFIFFSGPQGSGK
SFTSIQIYNHLMEKYGGEKSIGYASIDDFYLTHEDQLKLNEQFKNNKLLQGRGLPGTH
DMKLLQEVLNTIFNNNEHPDQDTVVLPKYDKSQFKGEGDRCPTGQKIKLPVDIFILEG
WFLGFNPILQGIENNDLLTGDMVDVNAKLFFYSDLLWRNPEIKSLGIVFTTDNINNVY
GWRLQQEHELISKVGKGMTDEQVHAFVDRYMPSYKLYLNDFVRSESLGSIATLTLGID
SNRNVYSTKTRCIE"
gene <910432..>910737
/gene="MVB12"
/locus_tag="YGR206W"
/db_xref="GeneID:853120"
mRNA <910432..>910737
/gene="MVB12"
/locus_tag="YGR206W"
/product="ubiquitin-binding ESCRT-I subunit protein MVB12"
/transcript_id="NM_001181335.3"
/db_xref="GeneID:853120"
CDS 910432..910737
/gene="MVB12"
/locus_tag="YGR206W"
/experiment="EXISTENCE:direct assay:GO:0000813 ESCRT I
complex [PMID:17145965|PMID:17135292]"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:17135292|PMID:14562095|PMID:17145965]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762|PMID:17145965]"
/experiment="EXISTENCE:direct assay:GO:0043130 ubiquitin
binding [PMID:19380877]"
/experiment="EXISTENCE:mutant phenotype:GO:0006623 protein
targeting to vacuole [PMID:17135292|PMID:17145965]"
/experiment="EXISTENCE:mutant phenotype:GO:0031333
negative regulation of protein-containing complex assembly
[PMID:17145965]"
/experiment="EXISTENCE:mutant phenotype:GO:1904669 ATP
export [PMID:26585826]"
/experiment="EXISTENCE:physical interaction:GO:0000813
ESCRT I complex [PMID:17135292|PMID:17145965]"
/note="ESCRT-I subunit required to stabilize ESCRT-I core
complex oligomers; the ESCRT-I core complex (Stp22p,
Vps28p, Srn2p) is involved in ubiquitin-dependent sorting
of proteins into the endosome; deletion mutant is
sensitive to rapamycin and nystatin"
/codon_start=1
/product="ubiquitin-binding ESCRT-I subunit protein MVB12"
/protein_id="NP_011722.3"
/db_xref="GeneID:853120"
/db_xref="SGD:S000003438"
/translation="MNNNVEELLRRIPLYNKYGKDFPQETVTRFQMPEFKLPALQPTR
DLLCPWYEECDNITKVCQLHDSSNKKFDQWYKEQYLSKKPPGIVGNTLLSPSRKDNS"
gene complement(<910843..>911628)
/gene="CIR1"
/locus_tag="YGR207C"
/db_xref="GeneID:853121"
mRNA complement(<910843..>911628)
/gene="CIR1"
/locus_tag="YGR207C"
/product="Cir1p"
/transcript_id="NM_001181336.1"
/db_xref="GeneID:853121"
CDS complement(910843..911628)
/gene="CIR1"
/locus_tag="YGR207C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:14576278|PMID:14562095|PMID:24769239|PMID:16823961]"
/note="Mitochondrial protein that interacts with frataxin
(Yfh1p); putative ortholog of mammalian electron transfer
flavoprotein complex subunit ETF-beta; may have a role in
oxidative stress response"
/codon_start=1
/product="Cir1p"
/protein_id="NP_011723.1"
/db_xref="GeneID:853121"
/db_xref="SGD:S000003439"
/translation="MSAKQQLRILVPVKRVVDFQIKPRVNKTLTGIETSGIKFSINPF
DDIAVEEAIRIKEKNKSLVESTHAVSIGSAKAQDILRNCLAKGIDTCSLIDSVGKENI
EPLAIAKILKAVVEKKGSNLVLMGKQAIDDDCNNTGQMLAGLLNWPQATNAAKVEFLD
NGRVQVTREIDDGEEVIEASLPMVITTDLRLNTPRYVGLPKLMKAKKKPIEKLDIAKD
FPEINIEPQLKIVSMEEPKTKSPGVKLNSVDELIEKLKEVKAI"
gene <911883..>912812
/gene="SER2"
/locus_tag="YGR208W"
/db_xref="GeneID:853122"
mRNA <911883..>912812
/gene="SER2"
/locus_tag="YGR208W"
/product="phosphoserine phosphatase"
/transcript_id="NM_001181337.1"
/db_xref="GeneID:853122"
CDS 911883..912812
/gene="SER2"
/locus_tag="YGR208W"
/EC_number="3.1.3.3"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0006564
L-serine biosynthetic process [PMID:1326413]"
/experiment="EXISTENCE:mutant phenotype:GO:0036424
L-phosphoserine phosphatase activity [PMID:1326413]"
/note="Phosphoserine phosphatase of the phosphoglycerate
pathway; involved in serine and glycine biosynthesis,
expression is regulated by the available nitrogen source"
/codon_start=1
/product="phosphoserine phosphatase"
/protein_id="NP_011724.1"
/db_xref="GeneID:853122"
/db_xref="SGD:S000003440"
/translation="MSKFVITCIAHGENLPKETIDQIAKEITESSAKDVSINGTKKLS
ARATDIFIEVAGSIVQKDLKNKLTNVIDSHNDVDVIVSVDNEYRQAKKLFVFDMDSTL
IYQEVIELIAAYAGVEEQVHEITERAMNNELDFKESLRERVKLLQGLQVDTLYDEIKQ
KLEVTKGVPELCKFLHKKNCKLAVLSGGFIQFAGFIKDQLGLDFCKANLLEVDTDGKL
TGKTLGPIVDGQCKSETLLQLCNDYNVPVEASCMVGDGGNDLPAMATAGFGIAWNAKP
KVQKAAPCKLNTKSMTDILYILGYTDDEIYNRQ"
gene complement(<912913..>913227)
/gene="TRX2"
/locus_tag="YGR209C"
/gene_synonym="LMA1"
/db_xref="GeneID:853123"
mRNA complement(<912913..>913227)
/gene="TRX2"
/locus_tag="YGR209C"
/gene_synonym="LMA1"
/product="thioredoxin TRX2"
/transcript_id="NM_001181338.3"
/db_xref="GeneID:853123"
CDS complement(912913..913227)
/gene="TRX2"
/locus_tag="YGR209C"
/gene_synonym="LMA1"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:8603912]"
/experiment="EXISTENCE:direct assay:GO:0006888 endoplasmic
reticulum to Golgi vesicle-mediated transport
[PMID:9813082]"
/experiment="EXISTENCE:direct assay:GO:0006890 retrograde
vesicle-mediated transport, Golgi to endoplasmic reticulum
[PMID:9813082]"
/experiment="EXISTENCE:direct assay:GO:0042144 vacuole
fusion, non-autophagic [PMID:9657146]"
/experiment="EXISTENCE:direct assay:GO:0080058 protein
deglutathionylation [PMID:20074363]"
/experiment="EXISTENCE:genetic interaction:GO:0000103
sulfate assimilation [PMID:2026619]"
/experiment="EXISTENCE:genetic interaction:GO:0006749
glutathione metabolic process [PMID:23242256]"
/experiment="EXISTENCE:genetic interaction:GO:0045454 cell
redox homeostasis [PMID:22561702]"
/experiment="EXISTENCE:genetic interaction:GO:0080058
protein deglutathionylation [PMID:20074363]"
/experiment="EXISTENCE:mutant phenotype:GO:0000011 vacuole
inheritance [PMID:8603912|PMID:9015301]"
/experiment="EXISTENCE:mutant phenotype:GO:0080058 protein
deglutathionylation [PMID:27708136]"
/note="Cytoplasmic thioredoxin isoenzyme; part of the
thioredoxin system that protects cells from oxidative and
reductive stress; subunit of the LMA1 complex, with Pbi2p,
that promotes vacuole inheritance; cofactor for Tsa1p;
required for ER-Golgi transport; facilitates mitochondrial
import of small Tims (Tim9p, Tim10p, Tim13p) by
maintaining them in reduced form along with Trx1p;
component of a signal transduction redox relay in a light
sensing pathway; abundance increases under DNA replication
stress"
/codon_start=1
/product="thioredoxin TRX2"
/protein_id="NP_011725.3"
/db_xref="GeneID:853123"
/db_xref="SGD:S000003441"
/translation="MVTQLKSASEYDSALASGDKLVVVDFFATWCGPCKMIAPMIEKF
AEQYSDAAFYKLDVDEVSDVAQKAEVSSMPTLIFYKGGKEVTRVVGANPAAIKQAIAS
NV"
gene complement(<913503..>914738)
/locus_tag="YGR210C"
/db_xref="GeneID:853124"
mRNA complement(<913503..>914738)
/locus_tag="YGR210C"
/product="uncharacterized protein"
/transcript_id="NM_001181339.1"
/db_xref="GeneID:853124"
CDS complement(913503..914738)
/locus_tag="YGR210C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to the cytoplasm"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011726.1"
/db_xref="GeneID:853124"
/db_xref="SGD:S000003442"
/translation="MPRDPLIGIVGKPSSGKSTTLNSLTDAGAAVGAFPFTTIEPNQA
TGYLQVECACSRFGKEDLCKPNYGWCSKGKRHIPIKLLDVAGLVPGAHSGRGLGNKFL
DDLRHADALIHVVDVSGTTDAEGKNTRGYDPLNDIEWLQDEIRLWVEGNLKKRWGSIV
RKHTATKSSIVDTLQAQFGGYGSHAPMIQKALDRLKGLPPLEKWDDEWITRVVKSFMM
EKFPTVLALNKIDHPDADKNVSKIMLKYPDTKAVLTSAVTEVFLRKLKKQGFILYEEG
TEFVDTFEDEPDKLKPLDDKILNRIENIRDLVLYRFGSTGVVQVLQAATDILGLIPVY
TVKNIQTFTGGNGTNVFRDCFLVKRGTPVGKVARYIMGGEVTIASIETVGGVRVSEES
LVEPGKNDILGFKIAPRSA"
gene <915241..>916701
/gene="ZPR1"
/locus_tag="YGR211W"
/db_xref="GeneID:853125"
mRNA <915241..>916701
/gene="ZPR1"
/locus_tag="YGR211W"
/product="zinc finger-containing protein ZPR1"
/transcript_id="NM_001181340.3"
/db_xref="GeneID:853125"
CDS 915241..916701
/gene="ZPR1"
/locus_tag="YGR211W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9852145|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:9852145|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0061770 translation
elongation factor binding [PMID:9852145]"
/experiment="EXISTENCE:mutant phenotype:GO:0000086 G2/M
transition of mitotic cell cycle [PMID:9852145]"
/experiment="EXISTENCE:mutant phenotype:GO:0006458 'de
novo' protein folding [PMID:36630955]"
/experiment="EXISTENCE:mutant phenotype:GO:0044183 protein
folding chaperone [PMID:36630955]"
/note="Chaperone with two zinc fingers essential for eEF1A
biogenesis; nuclear in growing cells, cytoplasmic in
starved cells; shuttled via process mediated by Cpr1p;
binds translation elongation factor eEF-1 (Tef1p);
relative distribution to nucleus increases upon DNA
replication stress; human ZPR1 gene can complement yeast
by allowing growth during down-regulation of yeast zpr1"
/codon_start=1
/product="zinc finger-containing protein ZPR1"
/protein_id="NP_011727.3"
/db_xref="GeneID:853125"
/db_xref="SGD:S000003443"
/translation="MSEQKEDLFKPVGEAAAEVEDESIAEQNKANDGVKLTGAQDAMG
HPVQEIESLCMNCGKNGTTRLLLTSIPYFREIIIMSFDCPHCGFKNCEIQPASQIQEK
GSRYVLKVECREDFNRQVIKSETATCKFVELDIEIPAKRGQLTTVEGLLSEMIDDLSQ
DQEMRKSIDEALYKKIDDFIQKVKSYINCEPNTIPITFILDDPAGNSWIEYKPGEPQH
KWSHTQYVRTDEQNVQVGIITRDQLEQRRQEQLKQLANRERNPSESVKVGSANPQFLS
DATDIENFNNEVQTFRASCPSCTQECETHMKPVNIPHFKEVIIMSTVCDHCGYKSNEV
KTGGAIPDKGRRITLYCDDAADLSRDILKSETCSMVIPELHLDIQEGTLGGRFTTLEG
LLRQVYEELESRIFTQTSDSMDEATKARWVEFFAKLKEAIAGKVKFTVIMEDPLAGSY
IQNVYAPDPDPNMTIEDYERTKEQNEDLGLSDIKVE"
gene <917040..>918446
/gene="SLI1"
/locus_tag="YGR212W"
/db_xref="GeneID:853126"
mRNA <917040..>918446
/gene="SLI1"
/locus_tag="YGR212W"
/product="N-acetyltransferase"
/transcript_id="NM_001181341.3"
/db_xref="GeneID:853126"
CDS 917040..918446
/gene="SLI1"
/locus_tag="YGR212W"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:15025559]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:15025559]"
/experiment="EXISTENCE:direct assay:GO:0008080
N-acetyltransferase activity [PMID:15025559]"
/experiment="EXISTENCE:direct assay:GO:0009410 response to
xenobiotic stimulus [PMID:15025559]"
/experiment="EXISTENCE:mutant phenotype:GO:0008080
N-acetyltransferase activity [PMID:15025559]"
/note="N-acetyltransferase; confers resistance to the
sphingolipid biosynthesis inhibitor myriocin (ISP-1) by
converting it into N-acetyl-myriocin, co-operates with
Ypk1p in mediating resistance to myriocin"
/codon_start=1
/product="N-acetyltransferase"
/protein_id="NP_011728.3"
/db_xref="GeneID:853126"
/db_xref="SGD:S000003444"
/translation="MNLKLSAIESYFFHRSRLNLHSCFYVGIKLNELPKKSQLIAALK
YTVIQHERLTCNVFYDELKKENFLQNILEPLKFCDLVEYRHDWDQLGETEINHIFQRY
NFSYNENKPLWKILILPNQNQMLLLTDHVLMDGMSAIHVWETFMEGLQMQQPVEIDET
IYSPSLNSSTEKIMSAPLYGDWPIPWNWHIVRQLVSRLHYWFPQTVVKNNRNLIQFAN
YSFPKDLLDDKPSDGTQKYKVKNTNHQWEFRLSPTHLNDILQECKANNTSLTSLLGAL
VCTSFEKIAAHEYTGSFLKIELPMNIRKPFERVLKLPSDDKLAVGNFIAVIEFNHKLH
QNRGIWDIASQIQRAIRSSSEDKIIDKVNEVKLLEVISSQQYIEDKISLNNGPSSTFE
VTNLGFQTFKDACNTSLPFYIVDATFNEPQGISSIFSLSVISTPGNGLHCCISYPNTL
TKVLEPHWQYMKDYLNLY"
gene complement(<918512..>919465)
/gene="RTA1"
/locus_tag="YGR213C"
/db_xref="GeneID:853127"
mRNA complement(<918512..>919465)
/gene="RTA1"
/locus_tag="YGR213C"
/product="Rta1p"
/transcript_id="NM_001181342.1"
/db_xref="GeneID:853127"
CDS complement(918512..919465)
/gene="RTA1"
/locus_tag="YGR213C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:22129104]"
/note="Protein involved in 7-aminocholesterol resistance;
has seven potential membrane-spanning regions; expression
is induced under both low-heme and low-oxygen conditions;
member of the fungal lipid-translocating exporter (LTE)
family of protein; RTA1 has a paralog, YLR046C, that arose
from the whole genome duplication"
/codon_start=1
/product="Rta1p"
/protein_id="NP_011729.1"
/db_xref="GeneID:853127"
/db_xref="SGD:S000003445"
/translation="MAKDGFELYRYTPELGASILFTVLFAVSGVAFVILLFHYSVKSK
RRVGSLMKSQPVLRYYGTVNLAGAYIPFIFGCFVECVGFAFRCKSSKDTTLLNPYIIQ
TVFLLVSPTLYAASIYMIFGRMATLLFAENLMIMPARFNTTIFVIGDVGSLLLQAIGG
AMMSKVTSASSGSHLVTAGLFIQIAFFGLFIINEVLFIFKMSKKPTNVSVRYGSWKYL
NIALLVNSFLILIRSIVRAVEFIQGYDGEIASHEWYLYIFDGLPMFLLVLIFIVAFPL
INIFRIHEESIQAQQSARFDGTDYPDVEVTSIEEDLASKSE"
gene <920575..>921788
/gene="RPS0A"
/locus_tag="YGR214W"
/gene_synonym="NAB1; NAB1A; YST1"
/db_xref="GeneID:853128"
mRNA join(<920575..920664,921120..>921788)
/gene="RPS0A"
/locus_tag="YGR214W"
/gene_synonym="NAB1; NAB1A; YST1"
/product="40S ribosomal protein uS2 RPS0A"
/transcript_id="NM_001181343.1"
/db_xref="GeneID:853128"
CDS join(920575..920664,921120..921788)
/gene="RPS0A"
/locus_tag="YGR214W"
/gene_synonym="NAB1; NAB1A; YST1"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:8626693]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405]"
/experiment="EXISTENCE:direct assay:GO:0022627 cytosolic
small ribosomal subunit [PMID:8626693]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/experiment="EXISTENCE:genetic interaction:GO:0000054
ribosomal subunit export from nucleus [PMID:16246728]"
/experiment="EXISTENCE:mutant phenotype:GO:0000028
ribosomal small subunit assembly [PMID:8626693]"
/experiment="EXISTENCE:mutant phenotype:GO:0000447
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from
5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:14627813]"
/experiment="EXISTENCE:mutant phenotype:GO:0000461
endonucleolytic cleavage to generate mature 3'-end of
SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:14627813]"
/experiment="EXISTENCE:mutant phenotype:GO:0002181
cytoplasmic translation [PMID:9973221|PMID:8626693]"
/note="Ribosomal 40S subunit protein S0A; required for
maturation of 18S rRNA along with Rps0Bp; deletion of
either RPS0 gene reduces growth rate, deletion of both
genes is lethal; homologous to human ribosomal protein SA
and bacterial S2; RPS0A has a paralog, RPS0B, that arose
from the whole genome duplication"
/codon_start=1
/product="40S ribosomal protein uS2 RPS0A"
/protein_id="NP_011730.1"
/db_xref="GeneID:853128"
/db_xref="SGD:S000003446"
/translation="MSLPATFDLTPEDAQLLLAANTHLGARNVQVHQEPYVFNARPDG
VHVINVGKTWEKLVLAARIIAAIPNPEDVVAISSRTFGQRAVLKFAAHTGATPIAGRF
TPGSFTNYITRSFKEPRLVIVTDPRSDAQAIKEASYVNIPVIALTDLDSPSEFVDVAI
PCNNRGKHSIGLIWYLLAREVLRLRGALVDRTQPWSIMPDLYFYRDPEEVEQQVAEEA
TTEEAGEEEAKEEVTEEQAEATEWAEENADNVEW"
gene <922175..>922507
/gene="RSM27"
/locus_tag="YGR215W"
/gene_synonym="mS33"
/db_xref="GeneID:853129"
mRNA <922175..>922507
/gene="RSM27"
/locus_tag="YGR215W"
/gene_synonym="mS33"
/product="mitochondrial 37S ribosomal protein mS33 RSM27"
/transcript_id="NM_001181344.3"
/db_xref="GeneID:853129"
CDS 922175..922507
/gene="RSM27"
/locus_tag="YGR215W"
/gene_synonym="mS33"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:11278769]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:11278769]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005763
mitochondrial small ribosomal subunit [PMID:11278769]"
/note="Mitochondrial ribosomal protein of the small
subunit"
/codon_start=1
/product="mitochondrial 37S ribosomal protein mS33 RSM27"
/protein_id="NP_011731.3"
/db_xref="GeneID:853129"
/db_xref="SGD:S000003447"
/translation="MNVPKARLLKVAELSAKIFDQNFNPSGIRTGSKILNERLKGPSV
ASYYGNPDILKFRHLKTLYPDIEFVDLEEQYRLSMVEAKKRRGKGAPKKMKKDAAATA
KGKGKKKK"
gene complement(<922633..>924462)
/gene="GPI1"
/locus_tag="YGR216C"
/db_xref="GeneID:853130"
mRNA complement(<922633..>924462)
/gene="GPI1"
/locus_tag="YGR216C"
/product="phosphatidylinositol
N-acetylglucosaminyltransferase"
/transcript_id="NM_001181345.1"
/db_xref="GeneID:853130"
CDS complement(922633..924462)
/gene="GPI1"
/locus_tag="YGR216C"
/EC_number="2.4.1.198"
/experiment="EXISTENCE:curator inference:GO:0017176
phosphatidylinositol N-acetylglucosaminyltransferase
activity [PMID:9729469]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0006506 GPI anchor
biosynthetic process [PMID:8910381]"
/experiment="EXISTENCE:genetic interaction:GO:0000506
glycosylphosphatidylinositol-N-
acetylglucosaminyltransferase (GPI-GnT) complex
[PMID:9729469]"
/experiment="EXISTENCE:mutant phenotype:GO:0006506 GPI
anchor biosynthetic process [PMID:8910381]"
/note="Membrane protein involved in the synthesis of
GlcNAc-PI; N-acetylglucosaminyl phosphatidylinositol
(GlcNAc-PI) is the first intermediate in the synthesis of
glycosylphosphatidylinositol (GPI) anchors; human and
mouse GPI1 are functional homologs"
/codon_start=1
/product="phosphatidylinositol
N-acetylglucosaminyltransferase"
/protein_id="NP_011732.1"
/db_xref="GeneID:853130"
/db_xref="SGD:S000003448"
/translation="MPNYIFWPYESLFENSAAQGPQVALAISFEKTHFVVLGVCEPQY
LEEVSIRPPYSVVATKNNGAEGWNYKVADPCNVHFRIPKLKFMQFYSSDPISLIIPEK
EVGLHSSVGETLNYSKLEQHPRYKRDNKKLSETLNIINLFPAYCKALNELYPFIQTSQ
ENLRGTMLNSVAAWCSSTCIYKMVAKIGFYLTFVICSIASLVSSLLNYSHFQLVNYSA
FVQQIDLRCQQICYFPVQYERINKKDNIQNVGSMVEKDNSNSQFSHSYMPSKFYPDYI
LLYNTIWLIINDISFGLILGAILIENRDFLVSASHRVLKFFLYDSLKTITETLANNPL
GIKLNAELANFLSELFLWVIEFSYTTFIKRLIDPKTLSSLLTLTIYMMFLVGFSFAVS
LAIDFFAILSFPIYVFYRISSKLYHCQLNIMASLFNLFCGKKRNVLRNRIDHNYFQLD
QLLLGTLLFIILVFLTPTVMAFYMSYTVLRMLTITIEIFSEAVIALINHFPLFALLLR
LKDPKRLPGGISIELKTTVSNKHTTLELQNNPIKFKSMFRPYNLLLSQMRTNYFSFAT
VRKIVRGESIMVNRNKLYYVLYSSLPSKPLSVKDLYKRLTIQA"
gene <924696..>930815
/gene="CCH1"
/locus_tag="YGR217W"
/db_xref="GeneID:853131"
mRNA <924696..>930815
/gene="CCH1"
/locus_tag="YGR217W"
/product="calcium channel protein CCH1"
/transcript_id="NM_001181346.3"
/db_xref="GeneID:853131"
CDS 924696..930815
/gene="CCH1"
/locus_tag="YGR217W"
/experiment="EXISTENCE:direct assay:GO:0005262 calcium
channel activity [PMID:10958666]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:10958666]"
/experiment="EXISTENCE:direct assay:GO:0006816 calcium ion
transport [PMID:10958666]"
/experiment="EXISTENCE:mutant phenotype:GO:0005262 calcium
channel activity [PMID:9343395]"
/experiment="EXISTENCE:mutant phenotype:GO:0006816 calcium
ion transport [PMID:9343395]"
/note="Voltage-gated high-affinity calcium channel;
involved in calcium influx in response to some
environmental stresses as well as exposure to mating
pheromones; interacts and partially co-localizes with
Mid1p; however, evidence suggests CCH1 is not required for
Mid1p function"
/codon_start=1
/product="calcium channel protein CCH1"
/protein_id="NP_011733.3"
/db_xref="GeneID:853131"
/db_xref="SGD:S000003449"
/translation="MQGRKRTLTEPFEPNTNPFGDNAAVMTENVEDNSETDGNRLESK
PQALVPPALNIVPPESSIHSTEEKKGDEYNGNDKDSSLISNIFRTRVGRSSHENLSRP
KLSLKTASFGAAESSRRNVSPSTKSAKSSSQYIDLNDERLRRRSFSSYSRSSSRRVSN
SPSSTDRPPRSAKVLSLIAADDMDDFEDLQKGFKSAIDEEGLTWLPQLKSEKSRPVSD
VGEDRGEGEQESIPDVHTPNVGASATPGSIHLTPEPAQNGSVSEGLEGSINNSRKKPS
PKFFHHLSPQKEDKDQTEVIEYAEDILDFETLQRKLESRPFVLYGHSLGVFSPTNPLR
IKIARFLLHRRYSLLYNTLLTFYAILLAIRTYNPHNVVFLYRFSNWTDYFIFILSACF
TGNDIAKIIAFGFWDDSEMFKAYGREYKSILQRSGIMKLYIYLREKYGRKLIDFIIPF
RIISPGEETKYQRSSLSTSLTKPYGAKENQRPFGTPRAFARSSWNRIDLVSSVSFWLG
MFLSIKSYDTKTGIRIFKPLAILRILRLVNVDTGMPSILRGLKYGIPQLVNVSSMLVY
FWIFFGILGVQIFQGSFRRQCVWFNPEDPTDTYQYDMQFCGGYLDPVTKRKQNYIYED
GSEGSVSKGFLCPQYSKCVSNANPYNGRISFDNIVNSMELVFVIMSANTFTDLMYYTM
DSDEMAACLFFIVCIFVLTIWLLNLLIAVLVSSFEIANEEYKKKKFIYGSRKTGYVAR
IVTGYWKYFKLKANQTKFPNWSQKGLAIYSHVEFIFVILIICDIGMRASVKVSTSANC
NNILLKTDRGISIVLFIESLARLVLYLPNMWKFLTKPSYVYDFIISIITLVISCLAVE
GVLGHMYAWLSIFHISRFYRVIISFNLTKKLWKQILSNGVMIWNLSSFYFFFTFLVAI
IMAVYFEGVIPPEEMADQPFGMYSLPNSFLSLFIIGSTENWTDILYALQKHSPNISST
FFCSVFFIIWFLLSNSVILNIFIALISESMEVKEEEKRPQQIKHYLKFVYPQKIQEYT
HASLVARIRKKFFGGHRNEDTRDFKQFLMRGTAIMNIAQNMGELADEFKEPPSENLFK
KGLSKLTIGVPSLKRLRMFANNPFYKNSDVVFTETNDINGRTYILELNEYEDEKLDYL
KKYPLFNYSYYFFSPQHRFRRFCQRLVPPSTGKRTDGSRFFEDSTDLYNKRSYFHHIE
RDVFVFIFALATILLIVCSCYVTPLYRMHHKMGTWNWSSALDCAFIGAFSIEFIVKTV
ADGFIYSPNAYLRNPWNFIDFCVLISMWINLIAYLKNNGNLSRIFKGLTALRALRCLT
ISNTARQTFNLVMFDGLNKIFEAGLISLSLLFPFTVWGLSIFKGRLGTCNDGSLGRAD
CYNEYSNSVFQWDIMSPRVYQQPYLHLDSFASAFSSLYQIISLEGWVDLLENMMNSSG
IGTPATVMGSAGNALFLVLFNFLSMVFILNLFVSFIVNNQARTTGSAYFTIEEKAWLE
SQKLLSQAKPKAIPNLIELSRVRQFFYQLAVEKKNFYYASFLQVVLYLHIIMLLSRSY
NPGNLIGYQGVYFMFSTSVFLIQEALHMCGEGPRLYFRQKWNSIRLSIIIIAFIMNAV
AFHVPASHYWFHNIKGFFLLVIFLFIIPQNDTLTELLETAMASLPPILSLTYTWGVLF
LVYAIALNQIFGLTRLGSNTTDNINFRTVIKSMIVLFRCSFGEGWNYIMADLTVSEPY
CSSDDNSTYTDCGSETYAYLLLMSWNIISMYIFVNMFVSLIIGNFSYVYRSGGSRSGI
NRSEIKKYIEAWSKFDTDGTGELELSYLPRIMHSFDGPLSFKIWEGRLTIKSLVENYM
EVNPDDPYDVKIDLIGLNKELNTIDKAKIIQRKLQYRRFVQSIHYTNAYNGCIRFSDL
LLQIPLYTAYSARECLGIDQYVHHLYILGKVDKYLENQRNFDVLEMVVTRWKFHCRMK
RTIEPEWDVKDPTVSSHISNINVNLEPAPGILEREPIATPRMDYGVNNFMWSPRMNQD
STMEPPEEPIDNNDDSANDLIDR"
gene complement(930953..931023)
/locus_tag="YNCG0043C"
/db_xref="GeneID:853132"
tRNA complement(930953..931023)
/locus_tag="YNCG0043C"
/product="tRNA-Gly"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0030371 translation
repressor activity [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043555 regulation
of translation in response to stress [PMID:27609601]"
/note="Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:853132"
/db_xref="SGD:S000006582"
repeat_region complement(931171..931540)
/note="Ty4 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006961"
repeat_region 931688..932019
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006977"
gene <932541..>935795
/gene="CRM1"
/locus_tag="YGR218W"
/gene_synonym="KAP124; XPO1"
/db_xref="GeneID:853133"
mRNA <932541..>935795
/gene="CRM1"
/locus_tag="YGR218W"
/gene_synonym="KAP124; XPO1"
/product="exportin CRM1"
/transcript_id="NM_001181347.3"
/db_xref="GeneID:853133"
CDS 932541..935795
/gene="CRM1"
/locus_tag="YGR218W"
/gene_synonym="KAP124; XPO1"
/experiment="EXISTENCE:direct assay:GO:0000776 kinetochore
[PMID:19139260]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9891088]"
/experiment="EXISTENCE:direct assay:GO:0005816 spindle
pole body [PMID:18573877]"
/experiment="EXISTENCE:direct assay:GO:0006409 tRNA export
from nucleus [PMID:36099418]"
/experiment="EXISTENCE:direct assay:GO:0017070 U6 snRNA
binding [PMID:31189105]"
/experiment="EXISTENCE:direct assay:GO:0030619 U1 snRNA
binding [PMID:31189105]"
/experiment="EXISTENCE:direct assay:GO:0030620 U2 snRNA
binding [PMID:31189105]"
/experiment="EXISTENCE:direct assay:GO:0030621 U4 snRNA
binding [PMID:31189105]"
/experiment="EXISTENCE:direct assay:GO:0030623 U5 snRNA
binding [PMID:31189105]"
/experiment="EXISTENCE:direct assay:GO:0061608 nuclear
import signal receptor activity [PMID:9857197]"
/experiment="EXISTENCE:genetic interaction:GO:0034501
protein localization to kinetochore [PMID:19139260]"
/experiment="EXISTENCE:mutant phenotype:GO:0000055
ribosomal large subunit export from nucleus
[PMID:11313466]"
/experiment="EXISTENCE:mutant phenotype:GO:0006406 mRNA
export from nucleus [PMID:9323132]"
/experiment="EXISTENCE:mutant phenotype:GO:0006611 protein
export from nucleus [PMID:33481703|PMID:9323132]"
/experiment="EXISTENCE:mutant phenotype:GO:0051168 nuclear
export [PMID:31189105]"
/experiment="EXISTENCE:mutant phenotype:GO:0061608 nuclear
import signal receptor activity [PMID:9323132]"
/experiment="EXISTENCE:mutant phenotype:GO:0071528 tRNA
re-export from nucleus [PMID:33074312]"
/note="Nuclear export factor, exportin; major karyopherin
involved in export of proteins, RNAs, and ribosomal
subunits from the nucleus"
/codon_start=1
/product="exportin CRM1"
/protein_id="NP_011734.3"
/db_xref="GeneID:853133"
/db_xref="SGD:S000003450"
/translation="MEGILDFSNDLDIALLDQVVSTFYQGSGVQQKQAQEILTKFQDN
PDAWQKADQILQFSTNPQSKFIALSILDKLITRKWKLLPNDHRIGIRNFVVGMIISMC
QDDEVFKTQKNLINKSDLTLVQILKQEWPQNWPEFIPELIGSSSSSVNVCENNMIVLK
LLSEEVFDFSAEQMTQAKALHLKNSMSKEFEQIFKLCFQVLEQGSSSSLIVATLESLL
RYLHWIPYRYIYETNILELLSTKFMTSPDTRAITLKCLTEVSNLKIPQDNDLIKRQTV
LFFQNTLQQIATSVMPVTADLKATYANANGNDQSFLQDLAMFLTTYLARNRALLESDE
SLRELLLNAHQYLIQLSKIEERELFKTTLDYWHNLVADLFYEVQRLPATEMSPLIQLS
VGSQAISTGSGALNPEYMKRFPLKKHIYEEICSQLRLVIIENMVRPEEVLVVENDEGE
IVREFVKESDTIQLYKSEREVLVYLTHLNVIDTEEIMISKLARQIDGSEWSWHNINTL
SWAIGSISGTMSEDTEKRFVVTVIKDLLDLTVKKRGKDNKAVVASDIMYVVGQYPRFL
KAHWNFLRTVILKLFEFMHETHEGVQDMACDTFIKIVQKCKYHFVIQQPRESEPFIQT
IIRDIQKTTADLQPQQVHTFYKACGIIISEERSVAERNRLLSDLMQLPNMAWDTIVEQ
STANPTLLLDSETVKIIANIIKTNVAVCTSMGADFYPQLGHIYYNMLQLYRAVSSMIS
AQVAAEGLIATKTPKVRGLRTIKKEILKLVETYISKARNLDDVVKVLVEPLLNAVLED
YMNNVPDARDAEVLNCMTTVVEKVGHMIPQGVILILQSVFECTLDMINKDFTEYPEHR
VEFYKLLKVINEKSFAAFLELPPAAFKLFVDAICWAFKHNNRDVEVNGLQIALDLVKN
IERMGNVPFANEFHKNYFFIFVSETFFVLTDSDHKSGFSKQALLLMKLISLVYDNKIS
VPLYQEAEVPQGTSNQVYLSQYLANMLSNAFPHLTSEQIASFLSALTKQYKDLVVFKG
TLRDFLVQIKEVGGDPTDYLFAEDKENALMEQNRLEREKAAKIGGLLKPSELDD"
gene complement(<936074..>936883)
/gene="MRPL9"
/locus_tag="YGR220C"
/gene_synonym="uL3m"
/db_xref="GeneID:853135"
mRNA complement(<936074..>936883)
/gene="MRPL9"
/locus_tag="YGR220C"
/gene_synonym="uL3m"
/product="mitochondrial 54S ribosomal protein uL3m MRPL9"
/transcript_id="NM_001181349.1"
/db_xref="GeneID:853135"
CDS complement(936074..936883)
/gene="MRPL9"
/locus_tag="YGR220C"
/gene_synonym="uL3m"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:1597181]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:2060626]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005762
mitochondrial large ribosomal subunit [PMID:2060626]"
/note="Mitochondrial ribosomal protein of the large
subunit"
/codon_start=1
/product="mitochondrial 54S ribosomal protein uL3m MRPL9"
/protein_id="NP_011736.1"
/db_xref="GeneID:853135"
/db_xref="SGD:S000003452"
/translation="MSKFLQGSIFSISKLHVRYSSTRPFLVAPSIANSITTEAPAINH
SPELANARKWLPKRCGLITRKKGMMPYFDKSTGERSAATILEVNNVEVIMHRTSEVNG
YFACQVGYGSRHLSKVSRQMLGHFASKVVNPKEHVAEFRVKDEKGLIPPGTLLKPSFF
KEGQYVDVRSVSKGKGFTGVMKRYGFKGLRASHGTSIMHRHGGSYGQNQDPGRVLPGR
KMPGHMGNEHVTIQNVKVLKVDDENNVIWVKGSVAGPKNSFVKIQDAIKKT"
gene complement(<937124..>938992)
/gene="TOS2"
/locus_tag="YGR221C"
/db_xref="GeneID:853136"
mRNA complement(<937124..>938992)
/gene="TOS2"
/locus_tag="YGR221C"
/product="Tos2p"
/transcript_id="NM_001181350.2"
/db_xref="GeneID:853136"
CDS complement(937124..938992)
/gene="TOS2"
/locus_tag="YGR221C"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:11489916]"
/experiment="EXISTENCE:direct assay:GO:0005933 cellular
bud [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:15282802|PMID:11489916]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:11489916|PMID:15282802]"
/experiment="EXISTENCE:genetic interaction:GO:0030427 site
of polarized growth [PMID:14872283]"
/experiment="EXISTENCE:genetic interaction:GO:0032507
maintenance of protein location in cell [PMID:14872283]"
/experiment="EXISTENCE:mutant phenotype:GO:1902413
negative regulation of mitotic cytokinesis
[PMID:16816427]"
/note="Protein involved in localization of Cdc24p to the
site of bud growth; may act as a membrane anchor;
localizes to the bud neck and bud tip; potentially
phosphorylated by Cdc28p; TOS2 has a paralog, SKG6, that
arose from the whole genome duplication"
/codon_start=1
/product="Tos2p"
/protein_id="NP_011737.2"
/db_xref="GeneID:853136"
/db_xref="SGD:S000003453"
/translation="MFSHYRYKENSCQKREAIPDKSRVSLTFLQKRTDSSNVTVAVAV
AVPIGAIIIVLSVVLIVVYRRCKKEPSMQDFDPNFEGDLYYLPKMDSSMNSANSDSNA
TEKRFIYGGYDDFLQPSIENSQSFKDYVRRINEHAPSAYNIASLASQNNSKLSVPSKH
IDLSNKISFESLENSELIVSPQHSNTGQDCDQRCDSTSNPDVNEKSSHNNDNRLKSNY
TSRSGLEPQCSREEEENIDRIRSIYNIYFEKSNSTIRSSVTSSIRRDSKLNIATRKSV
NMSSQDNPNDTTLIEQSHFGSTTVQEIDSSSSANEEYEDATDYLQVPAPQENKNIASS
VYSEVATREKVIPESSLSLTFPPPNGLSTRITSSIYSDTVAKDHIHSAKAPVRALSEG
SGQSNLTSAQQYSTYFIDHCNQSNDDNYYYNYPLPLEHPQNYENIGDLPTPTQFIYST
SSHSLTSFKGRPKPPKTLKHVPTARLNGTALNPMDHPEMFYSSPTKIPSTSLTKQFCT
PLPYQLRQSVVMTNPSELSMKPRYKPAGSLRNLIKAQYLPGNSSTTTSSSLSQPPSTL
SNAINFRVSGLLDDTDILQPPSVGEILPFKASTEDLRKQLGTSHNYEITPYENVHV"
gene complement(939459..939672)
/gene="SNR7-L"
/locus_tag="YNCG0044C"
/db_xref="GeneID:9164899"
ncRNA complement(939459..939672)
/ncRNA_class="snRNA"
/gene="SNR7-L"
/locus_tag="YNCG0044C"
/product="SNR7-L"
/experiment="EXISTENCE:direct assay:GO:0000245
spliceosomal complex assembly [PMID:24231520]"
/experiment="EXISTENCE:direct assay:GO:0000395 mRNA
5'-splice site recognition [PMID:18084028]"
/experiment="EXISTENCE:direct assay:GO:0005682 U5 snRNP
[PMID:11720284]"
/experiment="EXISTENCE:direct assay:GO:0030627 pre-mRNA
5'-splice site binding
[PMID:9430647|PMID:18084028|PMID:11453062]"
/experiment="EXISTENCE:direct assay:GO:0046540 U4/U6 x U5
tri-snRNP complex [PMID:10377396|PMID:10449419]"
/experiment="EXISTENCE:mutant phenotype:GO:0000350
generation of catalytic spliceosome for second
transesterification step [PMID:9430647]"
/experiment="EXISTENCE:mutant phenotype:GO:0000386 second
spliceosomal transesterification activity [PMID:9430647]"
/experiment="EXISTENCE:mutant phenotype:GO:0000398 mRNA
splicing, via spliceosome [PMID:3555841]"
/experiment="EXISTENCE:mutant phenotype:GO:0034403
alignment of 3' and 5' splice sites of mRNA
[PMID:9430647]"
/note="U5 spliceosomal RNA (U5 snRNA), long form;
associated with spliceosome"
/transcript_id="NR_132184.1"
/db_xref="GeneID:9164899"
/db_xref="SGD:S000006495"
gene complement(939494..939672)
/gene="SNR7-S"
/locus_tag="YNCG0045C"
/db_xref="GeneID:9164900"
ncRNA complement(939494..939672)
/ncRNA_class="snRNA"
/gene="SNR7-S"
/locus_tag="YNCG0045C"
/product="SNR7-S"
/experiment="EXISTENCE:direct assay:GO:0000245
spliceosomal complex assembly [PMID:24231520]"
/experiment="EXISTENCE:direct assay:GO:0000395 mRNA
5'-splice site recognition [PMID:18084028]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:2440583]"
/experiment="EXISTENCE:direct assay:GO:0005682 U5 snRNP
[PMID:11720284]"
/experiment="EXISTENCE:direct assay:GO:0030627 pre-mRNA
5'-splice site binding [PMID:18084028|PMID:9430647]"
/experiment="EXISTENCE:direct assay:GO:0046540 U4/U6 x U5
tri-snRNP complex [PMID:10377396|PMID:10449419]"
/experiment="EXISTENCE:mutant phenotype:GO:0000350
generation of catalytic spliceosome for second
transesterification step [PMID:9430647]"
/experiment="EXISTENCE:mutant phenotype:GO:0000386 second
spliceosomal transesterification activity [PMID:9430647]"
/experiment="EXISTENCE:mutant phenotype:GO:0000398 mRNA
splicing, via spliceosome [PMID:3555841]"
/experiment="EXISTENCE:mutant phenotype:GO:0034403
alignment of 3' and 5' splice sites of mRNA
[PMID:9430647]"
/note="U5 spliceosomal RNA (U5 snRNA), short form; derived
from SNR7-L by 3' end processing; associated with
spliceosome"
/transcript_id="NR_132185.1"
/db_xref="GeneID:9164900"
/db_xref="SGD:S000006496"
gene <939923..>940804
/gene="PET54"
/locus_tag="YGR222W"
/db_xref="GeneID:853137"
mRNA <939923..>940804
/gene="PET54"
/locus_tag="YGR222W"
/product="Pet54p"
/transcript_id="NM_001181351.3"
/db_xref="GeneID:853137"
CDS 939923..940804
/gene="PET54"
/locus_tag="YGR222W"
/experiment="EXISTENCE:direct assay:GO:0000372 Group I
intron splicing [PMID:18388132]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:8389363]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:8389363]"
/experiment="EXISTENCE:direct assay:GO:0048027 mRNA 5'-UTR
binding [PMID:18388132]"
/experiment="EXISTENCE:direct assay:GO:0097157 pre-mRNA
intronic binding [PMID:18388132]"
/experiment="EXISTENCE:mutant phenotype:GO:0000372 Group I
intron splicing [PMID:9341683]"
/experiment="EXISTENCE:mutant phenotype:GO:0045182
translation regulator activity [PMID:2548921]"
/experiment="EXISTENCE:mutant phenotype:GO:0070131
positive regulation of mitochondrial translation
[PMID:2548921]"
/experiment="EXISTENCE:mutant phenotype:GO:0090615
mitochondrial mRNA processing [PMID:9341683]"
/note="Mitochondrial inner membrane protein; binds to the
5' UTR of the COX3 mRNA to activate its translation
together with Pet122p and Pet494p; also binds to the COX1
Group I intron AI5 beta to facilitate exon ligation during
splicing; also stimulates efficient synthesis of Cox1p"
/codon_start=1
/product="Pet54p"
/protein_id="NP_011738.3"
/db_xref="GeneID:853137"
/db_xref="SGD:S000003454"
/translation="MKASSKAIKLVLDHLKSTGRVLGSVESGNSATISEKTASVNKQQ
QLQEKKPSVLQYRSYNPYLVKEDFLSILPENLYKKRGQFTNELDFQLMKVRDPKYFQF
KDQYYLFFNDYNSLTEYIKLTKHSRINKIRVKMTPLAQPLPTLLTKLQRYSKNLYNAF
RSSEQYFEGLNEKVDVSGEFTTNQLRSILDSVEEIENKSVLVWNIPTKLRSHDILNYF
WFYNIRSSFKIYWDDEMKRNLRFISFENSHDAYRFKRNYHGLLAKELLTLSEKGDAAD
YSLEMDDSKILIEHLSE"
gene complement(<940869..>942215)
/gene="HSV2"
/locus_tag="YGR223C"
/db_xref="GeneID:853138"
mRNA complement(<940869..>942215)
/gene="HSV2"
/locus_tag="YGR223C"
/product="phosphatidylinositol-3,5-bisphosphate binding
protein HSV2"
/transcript_id="NM_001181352.1"
/db_xref="GeneID:853138"
CDS complement(940869..942215)
/gene="HSV2"
/locus_tag="YGR223C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:15155809]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:15103325]"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:18769150]"
/experiment="EXISTENCE:direct assay:GO:0010314
phosphatidylinositol-5-phosphate binding [PMID:22704557]"
/experiment="EXISTENCE:direct assay:GO:0032266
phosphatidylinositol-3-phosphate binding
[PMID:22704557|PMID:15155809|PMID:22753491]"
/experiment="EXISTENCE:direct assay:GO:0070273
phosphatidylinositol-4-phosphate binding [PMID:15155809]"
/experiment="EXISTENCE:direct assay:GO:0080025
phosphatidylinositol-3,5-bisphosphate binding
[PMID:22753491|PMID:15103325|PMID:22704557]"
/experiment="EXISTENCE:mutant phenotype:GO:0032266
phosphatidylinositol-3-phosphate binding
[PMID:22704557|PMID:22753491]"
/experiment="EXISTENCE:mutant phenotype:GO:0034727
piecemeal microautophagy of the nucleus [PMID:18769150]"
/experiment="EXISTENCE:mutant phenotype:GO:0080025
phosphatidylinositol-3,5-bisphosphate binding
[PMID:22704557|PMID:22753491]"
/note="Phosphoinositide (PI) binding protein; involved in
micronucleophagy; belongs to the PROPPIN family of
proteins defined by a seven-bladed beta-propeller fold and
FRRG motif required for PI binding; displays punctate
cytoplasmic localization"
/codon_start=1
/product="phosphatidylinositol-3,5-bisphosphate binding
protein HSV2"
/protein_id="NP_011739.1"
/db_xref="GeneID:853138"
/db_xref="SGD:S000003455"
/translation="MDVRRPIREAVNNRRKPKFLSVSFNQDDSCFSVALENGFRIFNT
DPLTSKLSKTFKESATNQSRGTGIGYTRMLYRTNYIALVGGGKRPRHALNKLIIWDDL
LQKETITLKFMSSIKDVFLSRIHIVVVLENTIEIFQFQTNPQRICPILDIPPNGSVDY
VVCSSKHLQSQASQSQSKILEIIAFPSNKCVGQIQVADLSQIKYNSQNPKESALLPTS
IIKAHKNPIKLVRLNRQGTMVATCSVQGTLIRIFSTHNGTLIKEFRRGVDKADIYEMS
FSPNGSKLAVLSNKQTLHIFQIFETTNTETNTPDHSRANGSSHPLKNYIPKGLWRPKY
LDSVWSICNAHLKNPIFDAHRNDNSGDVTHDNEFYKDRCRIGWCQDSNNREQDDSLVL
VWQNSGIWEKFVILEKEQQDSSKTHYSLNESLRNEDTKSAGEPTRWELVRESWREL"
gene <942806..>944647
/gene="AZR1"
/locus_tag="YGR224W"
/db_xref="GeneID:853139"
mRNA <942806..>944647
/gene="AZR1"
/locus_tag="YGR224W"
/product="azole transporter"
/transcript_id="NM_001181353.3"
/db_xref="GeneID:853139"
CDS 942806..944647
/gene="AZR1"
/locus_tag="YGR224W"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:11113970]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0045117 azole
transmembrane transport [PMID:11113970]"
/experiment="EXISTENCE:mutant phenotype:GO:1901474 azole
transmembrane transporter activity [PMID:11113970]"
/note="Plasma membrane transporter of the major
facilitator superfamily; involved in resistance to azole
drugs such as ketoconazole and fluconazole"
/codon_start=1
/product="azole transporter"
/protein_id="NP_011740.3"
/db_xref="GeneID:853139"
/db_xref="SGD:S000003456"
/translation="MKGEPKTYSMSDLSYYGEKAQQQNEKQQKQYVVRRNSTQSTSKQ
NVSVVLEDNASESNELPKGFILYASLIALALSLFLAALDIMIVSTIIEEVAKQFGSYS
EIGWLFTGYSLPNALLALIWGRIATPIGFKETMLFAIVIFEIGSLISALANSMSMLIG
GRVIAGVGGCGIQSLSFVIGSTLVEESQRGILIAVLSCSFAIASVVGPFLGGVFTSSV
TWRWCFYVNLPIGGLAFFLFLFFYNPGLSTFQETMDNIRKFPSQFIEIVRNVAYHLLK
IKGFSKLNGWRKPFMELIFMYDIIEFVFCSAGFTCILLAFTFGGNRYAWNSASIIILF
IIGIVLVVLAGIYDFLVFPKFNIVKATPHYQPLMSWTNIKKPGIFTVNIALFLTCAGY
ISQFTYIVQYFQLIYNDSAWRAAVHLVACIISTVVTAILCGAITDKTRQIKPIIVISS
IFGVVGAGILTLLNNNANNSAHIGLLILPGVAFGGLAQSSMLASQIQLDKKSPTFRSD
FVSITTFNTFCKNLGQALGGVISNTVFSAAAIKKLTKANIQLPDGTTVDNLVIYRQTN
FDGSHSKLGNIISESLTDVFYMALGFYALSLIFAVFASNKKVTASLR"
gene <945145..>947019
/gene="AMA1"
/locus_tag="YGR225W"
/gene_synonym="SPO70"
/db_xref="GeneID:853140"
mRNA join(<945145..946327,946421..>947019)
/gene="AMA1"
/locus_tag="YGR225W"
/gene_synonym="SPO70"
/product="Ama1p"
/transcript_id="NM_001181354.3"
/db_xref="GeneID:853140"
CDS join(945145..946327,946421..947019)
/gene="AMA1"
/locus_tag="YGR225W"
/gene_synonym="SPO70"
/experiment="EXISTENCE:direct assay:GO:1990757 ubiquitin
ligase activator activity [PMID:15797379]"
/experiment="EXISTENCE:genetic interaction:GO:1903024
positive regulation of ascospore-type prospore membrane
formation [PMID:32788308]"
/experiment="EXISTENCE:mutant phenotype:GO:0007127 meiosis
I [PMID:11114178]"
/experiment="EXISTENCE:mutant phenotype:GO:0007130
synaptonemal complex assembly [PMID:23101628]"
/experiment="EXISTENCE:mutant phenotype:GO:0030437
ascospore formation [PMID:11114178]"
/experiment="EXISTENCE:mutant phenotype:GO:0030476
ascospore wall assembly [PMID:15590821]"
/experiment="EXISTENCE:mutant phenotype:GO:0044778 meiotic
DNA integrity checkpoint signaling [PMID:23101628]"
/experiment="EXISTENCE:mutant phenotype:GO:0045132 meiotic
chromosome segregation [PMID:23101628]"
/experiment="EXISTENCE:mutant phenotype:GO:0045732
positive regulation of protein catabolic process
[PMID:21118994]"
/experiment="EXISTENCE:mutant phenotype:GO:0051321 meiotic
cell cycle [PMID:11114178]"
/experiment="EXISTENCE:mutant phenotype:GO:1905786
positive regulation of anaphase-promoting
complex-dependent catabolic process [PMID:11114178]"
/experiment="EXISTENCE:physical interaction:GO:0005680
anaphase-promoting complex [PMID:11114178]"
/note="Activator of meiotic anaphase promoting complex
(APC/C); Cdc20p family member; required for initiation of
spore wall assembly; required for Clb1p degradation during
meiosis; prevents premature assembly of the meiosis I
spindle, required for DSB induced prophase I arrest"
/codon_start=1
/product="Ama1p"
/protein_id="NP_011741.5"
/db_xref="GeneID:853140"
/db_xref="SGD:S000003457"
/translation="MATPHLYHRYNSKSSNKNINSSGNSTEVDRFIPKSVSRNAYKSI
PMLNGFDISYSELCEKSPSPERLSSPEFFNELRNTGHYESISTTNEFSMSSISSSSES
QVTRSGSARASRNDYSKLTKEQKDHRKNIAHSLGFQLPDRVFTFETTSAEILEKNKAI
KNCFGPGSCAEIRSTFDFSTLSPDVARYYIANSNARSASPQRQIQRPAKRVKSHIPYR
VLDAPCLRNDFYSNLISWSRTTNNVLVGLGCSVYIWSEKEGAVSILDHQYLSEKRDLV
TCVSFCPYNTYFIVGTKFGRILLYDQKEFFHSSNTNEKEPVFVFQTESFKGICCLEWF
KPGEICKFYVGEENGNVSLFEIKSLHFPIKNWSKRQKLEDENLIGLKLHSTYQAQAQQ
VCGISLNEHANLLAVGGNDNSCSLWDISDLDKPIKKFVLPHKAAVKAIAFCPWSKSLL
ATGGGSKDRCIKFWHTSTGTLLDEICTSGQVTSLIWSLRHKQIVATFGFGDTKNPVLI
TLYSYPKLSKLLEVRSPNPLRVLSAVISPSSMAICVATNDETIRFYELWNDKEEIINE
IQESGIYGSNIIEYMEGIETTHNKRIR"
gene <947420..>948997
/gene="DIE2"
/locus_tag="YGR227W"
/gene_synonym="ALG10"
/db_xref="GeneID:853142"
mRNA <947420..>948997
/gene="DIE2"
/locus_tag="YGR227W"
/gene_synonym="ALG10"
/product="dolichyl-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-dolichol
alpha-1,2- glucosyltransferase"
/transcript_id="NM_001181356.2"
/db_xref="GeneID:853142"
CDS 947420..948997
/gene="DIE2"
/locus_tag="YGR227W"
/gene_synonym="ALG10"
/EC_number="2.4.1.256"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0106073 dolichyl
pyrophosphate Glc2Man9GlcNAc2
alpha-1,2-glucosyltransferase activity [PMID:9597543]"
/experiment="EXISTENCE:mutant phenotype:GO:0005789
endoplasmic reticulum membrane [PMID:9597543]"
/experiment="EXISTENCE:mutant phenotype:GO:0006487 protein
N-linked glycosylation [PMID:9597543]"
/note="Dolichyl-phosphoglucose-dependent
alpha-1,2-glucosyltransferase; located in the ER;
functions in pathway that synthesizes the dolichol-linked
oligosaccharide precursor for N-linked protein
glycosylation; has a role in regulation of ITR1 and INO1;
human homolog ALG10B can complement yeast die2 null
mutant"
/codon_start=1
/product="dolichyl-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-dolichol
alpha-1,2- glucosyltransferase"
/protein_id="NP_011743.2"
/db_xref="GeneID:853142"
/db_xref="SGD:S000003459"
/translation="MDAKKNTGEANNDVLEEEAAIQLIAPGIARNLTQEVITGIFCNV
VIYPLLLIYFVLTFRYMTTNIVPYEFIDEKFHVGQTLTYLKGKWTQWDPKITTPPGIY
ILGLINYYCIKPIFKSWSTLTILRLVNLLGGIIVFPILVLRPIFLFNALGFWPVSLMS
FPLMTTYYYLFYTDVWSTILILQSLSCVLTLPFGPVKSIWLSAFFAGVSCLFRQTNII
WTGFIMILAVERPAILQKQFNTHTFNNYLKLFIHAIDDFSNLVLPYMINFVLFFIYLI
WNRSITLGDKSSHSAGLHIVQIFYCFTFITVFSLPIWISRNFMKLYKLRIKRKPVQTF
FEFIGIMLIIRYFTKVHPFLLADNRHYTFYLFRRLIGNKSRLIKYFFMTPIYHFSTFA
YLEVMRPNQLTFHPITPLPIKEPVHLPIQLTHVSWTALITCTMVTIVPSPLFEPRYYI
LPYFFWRIFITCSCEPLIKDLKPAKEGENPITISSTKRLFMEFLWFMLFNVVTLVIFS
KVSFPWTTEPYLQRIIW"
gene complement(<949052..>949225)
/gene="OTO1"
/locus_tag="YGR227C-A"
/db_xref="GeneID:65924736"
mRNA complement(<949052..>949225)
/gene="OTO1"
/locus_tag="YGR227C-A"
/product="Oto1p"
/transcript_id="NM_001395731.1"
/db_xref="GeneID:65924736"
CDS complement(949052..949225)
/gene="OTO1"
/locus_tag="YGR227C-A"
/note="Small toxic protein; overexpression inhibits
ergosterol biosynthesis; not required for normal growth,
not conserved in related yeast species including S.
paradoxus"
/codon_start=1
/product="Oto1p"
/protein_id="NP_001382660.1"
/db_xref="GeneID:65924736"
/db_xref="SGD:S000303806"
/translation="MNVRGNQCIMSIRVFLKAGESSLSFAIKWLKRFEATTKKNQYIQ
NGWPLKDGNKKRK"
gene complement(<949382..>950899)
/gene="SMI1"
/locus_tag="YGR229C"
/gene_synonym="KNR4"
/db_xref="GeneID:853144"
mRNA complement(<949382..>950899)
/gene="SMI1"
/locus_tag="YGR229C"
/gene_synonym="KNR4"
/product="Smi1p"
/transcript_id="NM_001181358.1"
/db_xref="GeneID:853144"
CDS complement(949382..950899)
/gene="SMI1"
/locus_tag="YGR229C"
/gene_synonym="KNR4"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:10206705]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:10206705]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807]"
/experiment="EXISTENCE:genetic interaction:GO:0007346
regulation of mitotic cell cycle [PMID:11410349]"
/experiment="EXISTENCE:genetic interaction:GO:0032995
regulation of fungal-type cell wall biogenesis
[PMID:10206705]"
/experiment="EXISTENCE:mutant phenotype:GO:0007346
regulation of mitotic cell cycle [PMID:12823808]"
/experiment="EXISTENCE:mutant phenotype:GO:0032995
regulation of fungal-type cell wall biogenesis
[PMID:10206705]"
/note="Protein involved in the regulation of cell wall
synthesis; proposed to be involved in coordinating cell
cycle progression with cell wall integrity"
/codon_start=1
/product="Smi1p"
/protein_id="NP_011745.1"
/db_xref="GeneID:853144"
/db_xref="SGD:S000003461"
/translation="MDLFKRKVKEWVYSLSTDDHYAEYNPDETPTFNMGKRLNSNNGQ
VNPSQMHLNSVDEEMSMGFQNGVPSNEDINIDEFTSTESNDGVSETLLAWRHIDFWTS
EHNPDLNATLSDPCTQNDITHAEEDLEVSFPNPVKASFKIHDGQEDLESMTGTSGLFY
GFQLMTLDQVVAMTQAWRNVAKNLNKRSQQGLSHVTSTGSSSSMERLNGNKFKLPNIP
DQKSIPPNAVQPVYAHPAWIPLITDNAGNHIGVDLAPGPNGKYAQIITFGRDFDTKFV
IAENWGEFLLSFANDLEAGNWYLVDDNDDYFSGDGELVFRDKKSNGPIQDYFEVLKRR
TWIKYQENLRSQQQKSQPDTSLQEQKYVPASQKKVAAEQPSTLNAESIKGEDSGSADV
QSVQDHESVKIVKTEPSEAETTTVNTESLGQAEHEIKADNVDIKQESERKEDEKQPKV
EEKEHVENEHVTESAKKDDDVNKQTEEMNKKEENEIRSDDAKVEEAREEFENIAL"
gene <951894..>952307
/gene="BNS1"
/locus_tag="YGR230W"
/db_xref="GeneID:853145"
mRNA <951894..>952307
/gene="BNS1"
/locus_tag="YGR230W"
/product="Bns1p"
/transcript_id="NM_001181359.3"
/db_xref="GeneID:853145"
CDS 951894..952307
/gene="BNS1"
/locus_tag="YGR230W"
/experiment="EXISTENCE:genetic interaction:GO:0051321
meiotic cell cycle [PMID:10564265]"
/experiment="EXISTENCE:genetic interaction:GO:1904750
negative regulation of protein localization to nucleolus
[PMID:14551257]"
/note="hypothetical protein; FEAR network component that
partially overlaps the function of SPO12 in promoting
Cdc14p release from the nucleolus during early mitotic
anaphase and mitotic exit; overexpression partially
suppresses the meiotic defect of spo12; BNS1 has a
paralog, SPO12, that arose from the whole genome
duplication"
/codon_start=1
/product="Bns1p"
/protein_id="NP_011746.3"
/db_xref="GeneID:853145"
/db_xref="SGD:S000003462"
/translation="MSYGGSASQDIVTKLIRKIQNTSVSCTRRDENLDSFNQHLKMSL
KVAHNTKTFAKHCLHRQVFKNTYRKRKAVEDQRKNLNTQLRQKFASPSDNLLSPCSRK
LNDHKSKLFSAKSQPKTLEFVRGKQNIPRKPNVDI"
gene complement(<952548..>953480)
/gene="PHB2"
/locus_tag="YGR231C"
/db_xref="GeneID:853146"
mRNA complement(<952548..>953480)
/gene="PHB2"
/locus_tag="YGR231C"
/product="prohibitin subunit PHB2"
/transcript_id="NM_001181360.1"
/db_xref="GeneID:853146"
CDS complement(952548..953480)
/gene="PHB2"
/locus_tag="YGR231C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:9259555|PMID:24769239|PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:10835343|PMID:9632789]"
/experiment="EXISTENCE:direct assay:GO:0006457 protein
folding [PMID:10835343]"
/experiment="EXISTENCE:mutant phenotype:GO:0000001
mitochondrion inheritance [PMID:12882345]"
/experiment="EXISTENCE:mutant phenotype:GO:0000423
mitophagy [PMID:38964378]"
/experiment="EXISTENCE:mutant phenotype:GO:0045861
negative regulation of proteolysis [PMID:10207067]"
/note="Subunit of the prohibitin complex (Phb1p-Phb2p);
prohibitin is a 1.2 MDa ring-shaped inner mitochondrial
membrane chaperone that stabilizes newly synthesized
proteins; determinant of replicative life span; involved
in mitochondrial segregation; prohibitin deficiency
induces a mitochondrial unfolded protein response (mtUPR)"
/codon_start=1
/product="prohibitin subunit PHB2"
/protein_id="NP_011747.2"
/db_xref="GeneID:853146"
/db_xref="SGD:S000003463"
/translation="MNRSPGEFQRYAKAFQKQLSKVQQTGGRGQVPSPRGAFAGLGGL
LLLGGGALFINNALFNVDGGHRAIVYSRIHGVSSRIFNEGTHFIFPWLDTPIIYDVRA
KPRNVASLTGTKDLQMVNITCRVLSRPDVVQLPTIYRTLGQDYDERVLPSIVNEVLKA
VVAQFNASQLITQREKVSRLIRENLVRRASKFNILLDDVSITYMTFSPEFTNAVEAKQ
IAQQDAQRAAFVVDKARQEKQGMVVRAQGEAKSAELIGEAIKKSRDYVELKRLDTARD
IAKILASSPNRVILDNEALLLNTVVDARIDGRGK"
gene <953960..>954646
/gene="NAS6"
/locus_tag="YGR232W"
/db_xref="GeneID:853147"
mRNA <953960..>954646
/gene="NAS6"
/locus_tag="YGR232W"
/product="Nas6p"
/transcript_id="NM_001181361.3"
/db_xref="GeneID:853147"
CDS 953960..954646
/gene="NAS6"
/locus_tag="YGR232W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:19446323]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:19446323]"
/experiment="EXISTENCE:direct assay:GO:0044183 protein
folding chaperone [PMID:19412159]"
/experiment="EXISTENCE:direct assay:GO:1904855 proteasome
regulatory particle binding [PMID:11029046]"
/experiment="EXISTENCE:genetic interaction:GO:0070682
proteasome regulatory particle assembly
[PMID:19446323|PMID:19412159|PMID:19446322]"
/experiment="EXISTENCE:mutant phenotype:GO:0070682
proteasome regulatory particle assembly
[PMID:19446323|PMID:19446322]"
/note="Evolutionarily conserved 19S regulatory particle
assembly-chaperone; proteasome-interacting protein
involved in the assembly of the base subcomplex of the 19S
proteasomal regulatory particle (RP); ortholog of human
oncoprotein gankyrin, also known as p28, which interacts
with the Rb tumor suppressor and CDK4/6"
/codon_start=1
/product="Nas6p"
/protein_id="NP_011748.3"
/db_xref="GeneID:853147"
/db_xref="SGD:S000003464"
/translation="MSNYPLHQACMENEFFKVQELLHSKPSLLLQKDQDGRIPLHWSV
SFQAHEITSFLLSKMENVNLDDYPDDSGWTPFHIACSVGNLEVVKSLYDRPLKPDLNK
ITNQGVTCLHLAVGKKWFEVSQFLIENGASVRIKDKFNQIPLHRAASVGSLKLIELLC
GLGKSAVNWQDKQGWTPLFHALAEGHGDAAVLLVEKYGAEYDLVDNKGAKAEDVALNE
QVKKFFLNNV"
gene complement(<954674..>958210)
/gene="PHO81"
/locus_tag="YGR233C"
/gene_synonym="VAC6"
/db_xref="GeneID:853148"
mRNA complement(<954674..>958210)
/gene="PHO81"
/locus_tag="YGR233C"
/gene_synonym="VAC6"
/product="Pho81p"
/transcript_id="NM_001181362.3"
/db_xref="GeneID:853148"
CDS complement(954674..958210)
/gene="PHO81"
/locus_tag="YGR233C"
/gene_synonym="VAC6"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922|PMID:14562095|PMID:26928762|PMID:16611745]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0000425
pexophagy [PMID:38316984]"
/experiment="EXISTENCE:mutant phenotype:GO:0004861
cyclin-dependent protein serine/threonine kinase inhibitor
activity [PMID:11069666|PMID:7939631]"
/experiment="EXISTENCE:mutant phenotype:GO:0006796
phosphate-containing compound metabolic process
[PMID:4570606]"
/experiment="EXISTENCE:mutant phenotype:GO:0016036
cellular response to phosphate starvation [PMID:7939631]"
/note="Cyclin-dependent kinase (CDK) inhibitor; regulates
Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in
response to phosphate levels; inhibitory activity for
Pho80p-Pho85p requires myo-D-inositol heptakisphosphate
(IP7) generated by Vip1p; senses phosphate metabolites via
the SPX domain to regulate pexophagy in response to
phosphate starvation; relative distribution to the nucleus
increases upon DNA replication stress"
/codon_start=1
/product="Pho81p"
/protein_id="NP_011749.3"
/db_xref="GeneID:853148"
/db_xref="SGD:S000003465"
/translation="MKFGKYLEARQLELAEYNSHFIDYKALKKLIKQLAIPTLKASSD
LDLHLTLDDIDEKIIHQRLQENKAAFFFKLERELEKVNGYYLARESDLRIKFNILHSK
YKDYKINGKLNSNQATSFKNLYAAFKKFQKDLRNLEQYVELNKTGFSKALKKWDKRSQ
SHDKDFYLATVVSIQPIFTRDGPLKLNDETLHILLELNDIDNNNRRADIQSSTFTNDD
DDDNNTSNNNKHNNNNNNNNNNNNNNNNNNILHNNYELTTSKISENQLEHLFQASSSS
LDMEMEIENWYKEILNIATVKDVQRKHALLRNFRETKIFTYLLQNSSESFHKNVFSLL
KECLTTLFLLLVASPLDDNSLHIFYKSNQDHIDLSYCDEDDQVFSRKNVFHEAASCPE
KSRLFILDEALTTSKLSKETVQKLLNAQDIHSRVPLHYAAELGKLEFVHSLLITNLLE
DVDPIDSDSKTPLVLAITNNHIDVVRDLLTIGGANASPIEKPILDYSKNVISSTKVQF
DPLNVACKFNNHDAAKLLLEIRSKQNADNAKNKSSQHLCQPLFKKNSTGLCTLHIVAK
IGGDPQLIQLLIRYGADPNEIDGFNKWTPIFYAVRSGHSEVITELLKHNARLDIEDDN
GHSPLFYALWESHVDVLNALLQRPLNLPSAPLNEINSQSSTQRLNTIDLTPNDDKFDL
DIQDSIPDFALPPPIIPLRKYGHNFLEKKIFIKLKLRPGLESIKLTQDNGIIMSSSPG
RITLSSNLPEIIPRNVILPVRSGEINNFCKDISETNDEEDDDEISEDHDDGEIIFQVD
SIDDFSMDFEIFPSFGTRIIAKTTAMPFLFKKVAINSIATMNLPLFDTRLNNIGSLTL
DYQIIFPYPGNPLKIINYEPYWKSTGSDLMTSSKDGNFVTSSSLNGSFISVLVCALND
ETIVAAPKPYVEFKGTKILLNDLTKEQLEKVVDYDFGKIDGSFDEVTLKQYLSSRVVP
LRSLLEVIPGSAQLVIRVYFPTDKEIDTIPIKISPFININQFIDKLLLIIFEHERFLR
HSGSGSMRQIVFSSCNWEACSILNWKQPNFPVLLQMKNLLRDSTTGKFVGDTPNCLKE
LAVNPQKMSYLNTELINIHTMVQFAMNNNLLGVTLPYEVLKICPSLARIIKQNGLLLI
ASVGENDQIPADGGYSGIYYACELLFENNIDM"
gene <959904..>961103
/gene="YHB1"
/locus_tag="YGR234W"
/gene_synonym="YHB4"
/db_xref="GeneID:853149"
mRNA <959904..>961103
/gene="YHB1"
/locus_tag="YGR234W"
/gene_synonym="YHB4"
/product="flavohemoglobin"
/transcript_id="NM_001181363.1"
/db_xref="GeneID:853149"
CDS 959904..961103
/gene="YHB1"
/locus_tag="YGR234W"
/gene_synonym="YHB4"
/EC_number="1.14.12.17"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:15611069]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:15611069]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405]"
/experiment="EXISTENCE:direct assay:GO:0034599 cellular
response to oxidative stress [PMID:8810268|PMID:9545281]"
/experiment="EXISTENCE:mutant phenotype:GO:0016966 nitric
oxide reductase activity [PMID:10758168|PMID:15611069]"
/experiment="EXISTENCE:mutant phenotype:GO:0071218
cellular response to misfolded protein [PMID:27341336]"
/experiment="EXISTENCE:mutant phenotype:GO:1902170
cellular response to reactive nitrogen species
[PMID:27341336]"
/note="Nitric oxide oxidoreductase; flavohemoglobin that
plays role in oxidative and nitrosative stress responses;
protects against nitration of cellular targets and against
cell growth inhibition under aerobic or anaerobic
conditions; yeast flavohemoglobin Yhb1p and human homolog
neuroglobin NGB protect cells against alpha-synuclein
cytotoxicity and aggregate formation; protein increases in
abundance, relocalizes from nucleus to cytoplasmic foci
upon DNA replication stress"
/codon_start=1
/product="flavohemoglobin"
/protein_id="NP_011750.1"
/db_xref="GeneID:853149"
/db_xref="SGD:S000003466"
/translation="MLAEKTRSIIKATVPVLEQQGTVITRTFYKNMLTEHTELLNIFN
RTNQKVGAQPNALATTVLAAAKNIDDLSVLMDHVKQIGHKHRALQIKPEHYPIVGEYL
LKAIKEVLGDAATPEIINAWGEAYQAIADIFITVEKKMYEEALWPGWKPFDITAKEYV
ASDIVEFTVKPKFGSGIELESLPITPGQYITVNTHPIRQENQYDALRHYSLCSASTKN
GLRFAVKMEAARENFPAGLVSEYLHKDAKVGDEIKLSAPAGDFAINKELIHQNEVPLV
LLSSGVGVTPLLAMLEEQVKCNPNRPIYWIQSSYDEKTQAFKKHVDELLAECANVDKI
IVHTDTEPLINAAFLKEKSPAHADVYTCGSLAFMQAMIGHLKELEHRDDMIHYEPFGP
KMSTVQV"
gene complement(<961360..>962061)
/gene="MIC26"
/locus_tag="YGR235C"
/gene_synonym="MCS29; MIO27; MOS2"
/db_xref="GeneID:853150"
mRNA complement(<961360..>962061)
/gene="MIC26"
/locus_tag="YGR235C"
/gene_synonym="MCS29; MIO27; MOS2"
/product="Mic26p"
/transcript_id="NM_001181364.1"
/db_xref="GeneID:853150"
CDS complement(961360..962061)
/gene="MIC26"
/locus_tag="YGR235C"
/gene_synonym="MCS29; MIO27; MOS2"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:14562095|PMID:24769239|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0044284
mitochondrial crista junction [PMID:22009199]"
/experiment="EXISTENCE:direct assay:GO:0061617 MICOS
complex [PMID:22009199|PMID:21944719|PMID:21987634]"
/experiment="EXISTENCE:mutant phenotype:GO:0032460
negative regulation of protein oligomerization
[PMID:29733859]"
/experiment="EXISTENCE:mutant phenotype:GO:0042407 cristae
formation [PMID:22009199|PMID:21987634]"
/note="Component of the MICOS complex; MICOS (formerly
MINOS or MitOS) is a mitochondrial inner membrane complex
that extends into the intermembrane space and has a role
in the maintenance of crista junctions, inner membrane
architecture, and formation of contact sites to the outer
membrane; negative regulator of Mic10p oligomerization;
Mic26p is a non-essential component of the complex;
orthologous to human APOO and APOOL"
/codon_start=1
/product="Mic26p"
/protein_id="NP_011751.1"
/db_xref="GeneID:853150"
/db_xref="SGD:S000003467"
/translation="MTKDFYRQLDPVEEKIVPPENAIVISSEAKEATVNEKEAKQGVL
SQRVMKYIGENELVDGISVRDPDYLKRFFNERRKQFSAKWDKVTNKIDDIAGRYYARE
ESFTSTIASLHTDPNERLIPGLLSILVASMTGSVLARRRTWLLRATMPIILGSCCFAY
AMPTTFRNTMGLIHNLEMNTFPHFTERQDRVWKETKRLSTASVQYYYDAKKWLNKDVE
KTGNAIKNWTGVNVK"
gene complement(<962530..>962817)
/gene="SPG1"
/locus_tag="YGR236C"
/db_xref="GeneID:853151"
mRNA complement(<962530..>962817)
/gene="SPG1"
/locus_tag="YGR236C"
/product="Spg1p"
/transcript_id="NM_001181365.3"
/db_xref="GeneID:853151"
CDS complement(962530..962817)
/gene="SPG1"
/locus_tag="YGR236C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/note="Protein required for high temperature survival
during stationary phase; not required for growth on
nonfermentable carbon sources; the authentic, non-tagged
protein is detected in highly purified mitochondria in
high-throughput studies"
/codon_start=1
/product="Spg1p"
/protein_id="NP_011752.4"
/db_xref="GeneID:853151"
/db_xref="SGD:S000003468"
/translation="MKLDSGIYSEAQRVVRTPKFRYIMLGLVGAAVVPTAYMRRGYTV
PAHSLDNINGVDTTKASVMGTEQRAAMTKGKSLQEMMDDDEVTYLMFSSIM"
gene complement(<963298..>965655)
/locus_tag="YGR237C"
/db_xref="GeneID:853152"
mRNA complement(<963298..>965655)
/locus_tag="YGR237C"
/product="uncharacterized protein"
/transcript_id="NM_001181366.1"
/db_xref="GeneID:853152"
CDS complement(963298..965655)
/locus_tag="YGR237C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0032126 eisosome
[PMID:37387118]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to the cytoplasm"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011753.1"
/db_xref="GeneID:853152"
/db_xref="SGD:S000003469"
/translation="MSSAKTFTKHISFDDLAPSLIDDQATIIKNDSHHVGLNNHFLHI
PPQFNPVYKNTLTGSHGSNDLATDESLDSPEDEEASSPLQLGTPTSTTSGVPHFYTQV
FSPAAHDPSKSYLRSPSVERSRSESPMFRSRRRTSVRLPPPPKVSVLKKSRKAADEQG
PIDDIDIGDLDFELERKMTKMTERNTQKNSGSRKGYTQAAFANLNEVEDRIETKSMVD
LSESENMESSKKRSKSFAGMTDEELAKLEEFYISKGRSNKTKIDQFDFGEQVPVYLNT
TESKTDSSNVTDPLAAIYPSRPTIVHNAISMTIDHPDYENYISNTKEKLNCKDKDDDV
DLRVVSCYISGRRYTWSSVDWYVENLTRNGDHLVIITTIPEFEAKIDTLAYKEKRRHR
LERMTSNTSESMTTASHSLIGPDLSSPLSTGIRIEAIHNEAKQTCSDILNYYARRLAT
KIVRISIEMVKENSTRSAIISATSLYRPSLQVISTVSANIQIKFRNGKVKLPFFLMKH
FAMPAFVVPFEFIKPELLIKPRVDKDEQDNSDDLKTEVRKKERLQWLSALIRRTLENP
FTKHKVVDSDDEESDSDESVTSVNEYFPISPEKKEEMEFFDKMGYVRPKPSRQVLLDD
NTLMKYDSSGRKLTPIESRNSRRSSKRSSRIQFNNNGIYKVKSMVDDIYNHETASTPH
IKTALKWDNEDPKMKFTSHPMRKTKSAGLSPRTSSTSSSSGQRKAHHHHHHHNHVSRT
KTTESTKSGNSKKDSSSSSTNDHQFKRSEKKKKSKFGSIFKKVFG"
gene complement(<966039..>968687)
/gene="KEL2"
/locus_tag="YGR238C"
/db_xref="GeneID:853153"
mRNA complement(<966039..>968687)
/gene="KEL2"
/locus_tag="YGR238C"
/product="Kel2p"
/transcript_id="NM_001181367.3"
/db_xref="GeneID:853153"
CDS complement(966039..968687)
/gene="KEL2"
/locus_tag="YGR238C"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:9786949]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:9786949]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:9786949|PMID:19053807]"
/experiment="EXISTENCE:direct assay:GO:1990615
Kelch-containing formin regulatory complex
[PMID:24828508]"
/experiment="EXISTENCE:genetic interaction:GO:0032465
regulation of cytokinesis [PMID:24828508]"
/experiment="EXISTENCE:genetic interaction:GO:0090337
regulation of formin-nucleated actin cable assembly
[PMID:24828508]"
/experiment="EXISTENCE:mutant phenotype:GO:0000747
conjugation with cellular fusion [PMID:9786949]"
/experiment="EXISTENCE:mutant phenotype:GO:0001100
negative regulation of exit from mitosis [PMID:12234925]"
/experiment="EXISTENCE:mutant phenotype:GO:0060627
regulation of vesicle-mediated transport [PMID:24828508]"
/note="Protein that negatively regulates mitotic exit;
forms a complex with Kel1p and Bud14p that regulates Bnr1p
(formin) to affect actin cable assembly, cytokinesis, and
polarized growth; functions in a complex with Kel1p,
interacts with Tem1p and Lte1p; localizes to regions of
polarized growth; potential Cdc28p substrate"
/codon_start=1
/product="Kel2p"
/protein_id="NP_011754.3"
/db_xref="GeneID:853153"
/db_xref="SGD:S000003470"
/translation="MVPFKLTNKVPTDTGPSLISAQSVPRPIVFMDNRNNTRIVTPTL
PPNQHRGISGASTALPWSPESKNTGKYIWNRVKLKNSPFPRYRHSSSFIVTNDNRIFV
TGGLHDQSVYGDVWQIAANADGTSFTSKRIDIDQNTPPPRVGHASTICGNAYVVFGGD
THKLNKNGLLDDDLYLFNINSYKWTIPQPIGRRPLGRYGHKISIIASNPMQTKLYLFG
GQVDETYFNDLVVFDLSSFRRPNSHWEFLEPVGDLPPPLTNHTMVAYDNKLWVFGGET
PKTISNDTYRYDPAQSEWSKVKTTGEKPPPIQEHASVVYKHLMCVLGGKDTHNAYSND
VYFLNLLSLKWYKLPRMKEGIPQERSGHSLTLMKNEKLLIMGGDKTDYASPNIHDLQT
SETDQGEGTLLYTLDLSSLNELCPGIMCESLHAGESFSNSLSGGFTPSKSTESENQEI
INILTPRLPDSKVLSYNDIDEGAGSYSSALDDKAFERKSDREEKKPQSSKVDSSINKE
SPGTGIKVSKKNFPVLRGLTVDSEEYGSSSYKDTSCQKGIPKNLFDDLNLNLQTLRLE
AQQKELETARHISQLEKEVQRLMVIKEASKDSNFQTARLKNLEIQKTFLESRINDLKN
LLMVKLSQASKLCDQITIQNNGLKTCSEHVTIKRDIIDLENKCDVLKRQNEILVNNMQ
KITPELHTYLNESSCYLGKLLKSYPTSARPPSSEKDNQIYEKDSLNKIEKVINEMHET
VRAKEKLHLETQKLNDERDSLRANLLDNNNKLDALRKLSDGSSKSMDLTKKAIHLSQS
ELEKYRKNNDDLQKEIDRIKTEQAEQDDKQEQRGAITHGNFDAFHRMKINNLKAELYM
SKENRDSLKDELLALKKKLYTLEQKK"
gene complement(<969187..>970053)
/gene="PEX21"
/locus_tag="YGR239C"
/db_xref="GeneID:853154"
mRNA complement(<969187..>970053)
/gene="PEX21"
/locus_tag="YGR239C"
/product="Pex21p"
/transcript_id="NM_001181368.3"
/db_xref="GeneID:853154"
CDS complement(969187..970053)
/gene="PEX21"
/locus_tag="YGR239C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:9864360]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:9864360]"
/experiment="EXISTENCE:direct assay:GO:0051099 positive
regulation of binding [PMID:17451428]"
/experiment="EXISTENCE:genetic interaction:GO:0016558
protein import into peroxisome matrix [PMID:9864360]"
/experiment="EXISTENCE:mutant phenotype:GO:0016558 protein
import into peroxisome matrix [PMID:26276932]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:9864360]"
/note="Peroxin required for peroxisomal matrix protein
targeting; acts on proteins containing the PTS2 targeting
sequence; interacts with Pex7p; constitutively expressed;
partially redundant with Pex18p; required for import of
the Gpd1p-Pnc1p heterodimer in which only Gpd1p has a
peroxisomal targeting signal; relative distribution to
cytoplasmic foci increases upon DNA replication stress"
/codon_start=1
/product="Pex21p"
/protein_id="NP_011755.3"
/db_xref="GeneID:853154"
/db_xref="SGD:S000003471"
/translation="MPSVCHTSPIEKIIQQGHRIQNDSLIPSKRTKLAHTELTAHYAT
EDSHVEKHFLHNGSNFDGIDNVRYQNQPSPLTFITPNNTVDSSDWVPQFSSMKIDDSL
EFSSEYKRLYSNYESQQRLNSSRQHLPFKNCMIRKTSCTYPPQKTLRQQRQGNRDNPT
DAFQFDAEFQVLEREIQKERYEPITRRDEKWFDQDQSELQRIATDIVKCCTPPPSSAS
SSSTLSSSVESKLSESKFIQLMRNISSGDVTLKKNADGNSASELFSSNNGELVGNRHI
FVKDEIHKDILD"
gene complement(<970771..>973734)
/gene="PFK1"
/locus_tag="YGR240C"
/db_xref="GeneID:853155"
mRNA complement(<970771..>973734)
/gene="PFK1"
/locus_tag="YGR240C"
/product="6-phosphofructokinase subunit alpha"
/transcript_id="NM_001181369.1"
/db_xref="GeneID:853155"
CDS complement(970771..973734)
/gene="PFK1"
/locus_tag="YGR240C"
/EC_number="2.7.1.11"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922|PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16962558]"
/experiment="EXISTENCE:direct assay:GO:0005945
6-phosphofructokinase complex
[PMID:21241708|PMID:6231961|PMID:4271029]"
/experiment="EXISTENCE:direct assay:GO:0006096 glycolytic
process [PMID:3000145]"
/experiment="EXISTENCE:genetic interaction:GO:0003872
6-phosphofructokinase activity [PMID:3007939]"
/experiment="EXISTENCE:genetic interaction:GO:0006096
glycolytic process [PMID:2965996]"
/experiment="EXISTENCE:mutant phenotype:GO:0003872
6-phosphofructokinase activity [PMID:3007939]"
/experiment="EXISTENCE:mutant phenotype:GO:0006096
glycolytic process [PMID:2965996]"
/experiment="EXISTENCE:mutant phenotype:GO:0051453
regulation of intracellular pH [PMID:24860096]"
/note="Alpha subunit of heterooctameric
phosphofructokinase; involved in glycolysis, indispensable
for anaerobic growth, activated by
fructose-2,6-bisphosphate and AMP, mutation inhibits
glucose induction of cell cycle-related genes"
/codon_start=1
/product="6-phosphofructokinase subunit alpha"
/protein_id="NP_011756.1"
/db_xref="GeneID:853155"
/db_xref="SGD:S000003472"
/translation="MQSQDSCYGVAFRSIITNDEALFKKTIHFYHTLGFATVKDFNKF
KHGENSLLSSGTSQDSLREVWLESFKLSEVDASGFRIPQQEATNKAQSQGALLKIRLV
MSAPIDETFDTNETATITYFSTDLNKIVEKFPKQAEKLSDTLVFLKDPMGNNITFSGL
ANATDSAPTSKDAFLEATSEDEIISRASSDASDLLRQTLGSSQKKKKIAVMTSGGDSP
GMNAAVRAVVRTGIHFGCDVFAVYEGYEGLLRGGKYLKKMAWEDVRGWLSEGGTLIGT
ARSMEFRKREGRRQAAGNLISQGIDALVVCGGDGSLTGADLFRHEWPSLVDELVAEGR
FTKEEVAPYKNLSIVGLVGSIDNDMSGTDSTIGAYSALERICEMVDYIDATAKSHSRA
FVVEVMGRHCGWLALMAGIATGADYIFIPERAVPHGKWQDELKEVCQRHRSKGRRNNT
IIVAEGALDDQLNPVTANDVKDALIELGLDTKVTILGHVQRGGTAVAHDRWLATLQGV
DAVKAVLEFTPETPSPLIGILENKIIRMPLVESVKLTKSVATAIENKDFDKAISLRDT
EFIELYENFLSTTVKDDGSELLPVSDRLNIGIVHVGAPSAALNAATRAATLYCLSHGH
KPYAIMNGFSGLIQTGEVKELSWIDVENWHNLGGSEIGTNRSVASEDLGTIAYYFQKN
KLDGLIILGGFEGFRSLKQLRDGRTQHPIFNIPMCLIPATVSNNVPGTEYSLGVDTCL
NALVNYTDDIKQSASATRRRVFVCEVQGGHSGYIASFTGLITGAVSVYTPEKKIDLAS
IREDITLLKENFRHDKGENRNGKLLVRNEQASSVYSTQLLADIISEASKGKFGVRTAI
PGHVQQGGVPSSKDRVTASRFAVKCIKFIEQWNKKNEASPNTDAKVLRFKFDTHGEKV
PTVEHEDDSAAVICVNGSHVSFKPIANLWENETNVELRKGFEVHWAEYNKIGDILSGR
LKLRAEVAALAAENK"
gene complement(<974577..>974777)
/locus_tag="YGR240C-A"
/db_xref="GeneID:1466463"
mRNA complement(<974577..>974777)
/locus_tag="YGR240C-A"
/product="uncharacterized protein"
/transcript_id="NM_001184552.1"
/db_xref="GeneID:1466463"
CDS complement(974577..974777)
/locus_tag="YGR240C-A"
/note="hypothetical protein; identified by fungal homology
and RT-PCR"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878083.1"
/db_xref="GeneID:1466463"
/db_xref="SGD:S000028551"
/translation="MILSKHVNFTYHTYNLIYNFYPDLTNFGMPGSIYGLQSSLKTME
KHVEIPQSIHHYSPFYQLPLIF"
gene complement(<974875..>976581)
/gene="YAP1802"
/locus_tag="YGR241C"
/db_xref="GeneID:853157"
mRNA complement(<974875..>976581)
/gene="YAP1802"
/locus_tag="YGR241C"
/product="Yap1802p"
/transcript_id="NM_001181370.3"
/db_xref="GeneID:853157"
CDS complement(974875..976581)
/gene="YAP1802"
/locus_tag="YGR241C"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:33443082]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:26928762]"
/experiment="EXISTENCE:physical interaction:GO:0006897
endocytosis [PMID:9531549]"
/note="Protein of the AP180 family, involved in clathrin
cage assembly; binds Pan1p and clathrin; YAP1802 has a
paralog, YAP1801, that arose from the whole genome
duplication"
/codon_start=1
/product="Yap1802p"
/protein_id="NP_011757.3"
/db_xref="GeneID:853157"
/db_xref="SGD:S000003473"
/translation="MSSLYTKLVKGATKIKMAPPKQKYVDPILSGTSSARGLQEITHA
LDIRLSDTAWTIVYKALIVLHLMIQQGEKDVTLRHYSHNLDVFQLRKISHTTKWSSND
MRALQRYDEYLKTRCEEYGRLGMDHLRDNYSSLKLGSKNQLSMDEELDHVESLEIQIN
ALIRNKYSVSDLENHLLLYAFQLLVQDLLGLYNALNEGVITLLESFFELSIEHAKRTL
DLYKDFVDMTEYVVRYLKIGKAVGLKIPVIKHITTKLINSLEEHLREETKRQRGEPSE
PQQDRKPSTAISSTSSHNNNSNDKNKSIAQKKLEQIREQKRLLEQQLQNQQLLISPTV
PQDAYNPFGSQQQDLNNDTFSFEPTQPQMTAQVPQPTANPFLIPQQQQQALQLTSAST
MPQPSEIQITPNLNNQQTGMYASNLQYTPNFTGSGFGGYTTTENNAIMTGTLDPTKTG
SNNPFSLENIAREQQQQNFQNSPNPFTLQQAQTTPILAHSQTGNPFQAQNVVTSPMGT
YMTNPVAGQLQYASTGAQQQPQMMQGQQTGYVMVPTAFVPINQQQQQQQHQQENPNLI
DI"
gene <977336..>977776
/gene="MPC3"
/locus_tag="YGR243W"
/gene_synonym="FMP43"
/db_xref="GeneID:853158"
mRNA <977336..>977776
/gene="MPC3"
/locus_tag="YGR243W"
/gene_synonym="FMP43"
/product="mitochondrial pyruvate carrier"
/transcript_id="NM_001181372.1"
/db_xref="GeneID:853158"
CDS 977336..977776
/gene="MPC3"
/locus_tag="YGR243W"
/gene_synonym="FMP43"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14562095|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:40012703]"
/experiment="EXISTENCE:direct assay:GO:0006850 pyruvate
import into mitochondria [PMID:25672363]"
/experiment="EXISTENCE:direct assay:GO:0031966
mitochondrial membrane [PMID:22628558]"
/experiment="EXISTENCE:direct assay:GO:0050833 pyruvate
transmembrane transporter activity [PMID:25672363]"
/experiment="EXISTENCE:mutant phenotype:GO:0006850
pyruvate import into mitochondria [PMID:22628554]"
/experiment="EXISTENCE:mutant phenotype:GO:0050833
pyruvate transmembrane transporter activity
[PMID:22628554]"
/note="Highly conserved subunit of the mitochondrial
pyruvate carrier (MPC); expressed during growth on
nonfermentable carbon sources, and heterodimerizes with
Mpc1p to form the respiratory isoform of MPC; MPC
localizes to the mitochondrial inner membrane and mediates
pyruvate uptake; MPC3 paralog, MPC2, heterodimerizes with
Mpc1p to form the fermentative MPC isoform; protein
abundance increases in response to DNA replication stress"
/codon_start=1
/product="mitochondrial pyruvate carrier"
/protein_id="NP_011759.1"
/db_xref="GeneID:853158"
/db_xref="SGD:S000003475"
/translation="MSASAFNFAFRRFWNSETGPKTVHFWAPTLKWGLVFAGLNDIKR
PVEKVSGAQNLSLLATALIWTRWSFVIKPKNYLLASVNFFLGCTAGYHLTRIANFRIR
NGDSFKQVIHYIIKGETPAAVAAKQTASTSMNKGVIGTNPPITH"
rep_origin 977728..977976
/note="ARS733; Autonomously Replicating Sequence"
/db_xref="SGD:S000118467"
gene complement(<978036..>979319)
/gene="LSC2"
/locus_tag="YGR244C"
/db_xref="GeneID:853159"
mRNA complement(<978036..>979319)
/gene="LSC2"
/locus_tag="YGR244C"
/product="succinate--CoA ligase (GDP-forming) subunit
beta"
/transcript_id="NM_001181373.3"
/db_xref="GeneID:853159"
CDS complement(978036..979319)
/gene="LSC2"
/locus_tag="YGR244C"
/EC_number="6.2.1.5"
/experiment="EXISTENCE:direct assay:GO:0004775
succinate-CoA ligase (ADP-forming) activity
[PMID:9874242]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:24769239|PMID:14576278|PMID:9874242|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0006104
succinyl-CoA metabolic process [PMID:9874242]"
/experiment="EXISTENCE:mutant phenotype:GO:0004775
succinate-CoA ligase (ADP-forming) activity
[PMID:9874242]"
/note="Beta subunit of succinyl-CoA ligase; succinyl-CoA
ligase is a mitochondrial enzyme of the TCA cycle that
catalyzes the nucleotide-dependent conversion of
succinyl-CoA to succinate"
/codon_start=1
/product="succinate--CoA ligase (GDP-forming) subunit
beta"
/protein_id="NP_011760.3"
/db_xref="GeneID:853159"
/db_xref="SGD:S000003476"
/translation="MYSRKSLSLISKCGQLSRLNAQAALQARRHLSIHEYRSAQLLRE
YGIGTPEGFPAFTPEEAFEAAKKLNTNKLVIKAQALTGGRGKGHFDTGYKSGVHMIES
PQQAEDVAKEMLNHNLITKQTGIAGKPVSAVYIVKRVDTKHEAYLSILMDRQTKKPMI
IASSQGGMNIEEVAERTPDAIKKFSIETSKGLSPQMAKDVAKSLGFSPDAQDEAAKAV
SNLYKIFMERDATQVEINPLSEIEHDPTHKIMCTDAKFGFDDNASFRQEKIYSWRDLS
QEDPDEVKAKKYDLNFVKLKGNIGCLVNGAGLAMATMDVIKLNGGDPANFLDCGGGAT
PETIKQGFELILSNKNVDAIFVNIFGGIVRCDYVALGLVEAARELEVRVPIVARLQGT
KVEEGRDIINKSGVKIYSFDELDPAAKKVVELTQN"
gene complement(<979765..>982068)
/gene="SDA1"
/locus_tag="YGR245C"
/db_xref="GeneID:853160"
mRNA complement(<979765..>982068)
/gene="SDA1"
/locus_tag="YGR245C"
/product="Sda1p"
/transcript_id="NM_001181374.3"
/db_xref="GeneID:853160"
CDS complement(979765..982068)
/gene="SDA1"
/locus_tag="YGR245C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10704371]"
/experiment="EXISTENCE:mutant phenotype:GO:0000055
ribosomal large subunit export from nucleus
[PMID:15107621]"
/experiment="EXISTENCE:mutant phenotype:GO:0007089
traversing start control point of mitotic cell cycle
[PMID:11160833]"
/experiment="EXISTENCE:mutant phenotype:GO:0042273
ribosomal large subunit biogenesis [PMID:15107621]"
/note="Protein required for actin organization and passage
through Start; highly conserved nuclear protein; required
for actin cytoskeleton organization; plays a critical role
in G1 events; binds Nap1p; involved in 60S ribosome
biogenesis"
/codon_start=1
/product="Sda1p"
/protein_id="NP_011761.3"
/db_xref="GeneID:853160"
/db_xref="SGD:S000003477"
/translation="MGRRSRAAMLPTNIILLQNLVKRDPESYQEEFLQQYAHYESLRD
IFMLNGLAGGDSAAATNGLDVGNGSSTMAGTNGTTMSTSTSQLIELVGFVSQVCSCFP
RETANFPSELKQLLLEHHKSLPFELKEKILSCLTMLRNKDVITAEELIQSLFPLLVAY
SSHGNSLGVNSHAKELRKIIYTNLISLLKSCNTNGKNQKLNKSTQAVCFNLLDQPDSQ
GIWATKLTRELWRRGIWDDSRTVEIMTQAALHQDVKIVMSGVMFFLDADREREENFEE
NSEDEDGFDLDALRHKMQVNKKTGRRGKKLENAIKTVKKKKKNGPGAPQGYLNFSAIH
LLRDPQGFAEKLFKEHLSGKTKNKFDMEQKISLMQLLSRLIGTHKLIVLGIYTFFLKY
LTPKQRDVTRIMSACAQACHDLVPPEVINVMVRKIADEFVSDGVANEVAAAGINTIRE
ICSRAPLAIDEILLQDLVEYKGSKAKGVNMAAKSLIALYRDVAPEMLKKKDRGKNAAM
EVQEAKKGGKDSKRPQFGADNSVQGIAGIELLAKWKKEHGEESENEDADANWEVDVDS
EEDDVDGEWVTMDSDKEYDVDMEDSDDEKDNAKGKESDSDLELSDDDDEKEVKDEQED
ADIDPEAAFREIASTRILTPADFAKLQELRNEESVAKIMGIHKQDKREELVDASTLTG
PIKYKQSREERLQKVLEGREGRDKFGSRRGKRDNMRSTTNREKERRKNFVMSIHKRSV
RGKQKMSLRDKQKVLRAHITKQKKKGY"
gene complement(<982482..>984272)
/gene="BRF1"
/locus_tag="YGR246C"
/gene_synonym="PCF4; TDS4"
/db_xref="GeneID:853161"
mRNA complement(<982482..>984272)
/gene="BRF1"
/locus_tag="YGR246C"
/gene_synonym="PCF4; TDS4"
/product="transcription factor TFIIIB subunit BRF1"
/transcript_id="NM_001181375.1"
/db_xref="GeneID:853161"
CDS complement(982482..984272)
/gene="BRF1"
/locus_tag="YGR246C"
/gene_synonym="PCF4; TDS4"
/experiment="EXISTENCE:direct assay:GO:0000126
transcription factor TFIIIB complex
[PMID:1458536|PMID:1423590]"
/experiment="EXISTENCE:direct assay:GO:0000994 RNA
polymerase III core binding [PMID:9689057|PMID:7995525]"
/experiment="EXISTENCE:direct assay:GO:0001156
TFIIIC-class transcription factor complex binding
[PMID:12930823]"
/experiment="EXISTENCE:direct assay:GO:0003677 DNA binding
[PMID:9271407]"
/experiment="EXISTENCE:direct assay:GO:0017025 TBP-class
protein binding [PMID:15190063]"
/experiment="EXISTENCE:direct assay:GO:0070898 RNA
polymerase III preinitiation complex assembly
[PMID:11387215]"
/experiment="EXISTENCE:mutant phenotype:GO:0001112
DNA-templated transcription open complex formation
[PMID:11387215]"
/experiment="EXISTENCE:mutant phenotype:GO:0070898 RNA
polymerase III preinitiation complex assembly
[PMID:11387215|PMID:9218475|PMID:9710642]"
/experiment="EXISTENCE:physical interaction:GO:0000994 RNA
polymerase III core binding [PMID:7995525]"
/experiment="EXISTENCE:physical interaction:GO:0001156
TFIIIC-class transcription factor complex binding
[PMID:12930823]"
/note="TFIIIB B-related factor; one of three subunits of
RNA polymerase III transcription initiation factor TFIIIB,
binds TFIIIC and TBP and recruits RNA pol III to
promoters, amino-terminal half is homologous to TFIIB;
mutations in human homolog are associated with autosomal
recessive cerebellar-facial-dental syndrome"
/codon_start=1
/product="transcription factor TFIIIB subunit BRF1"
/protein_id="NP_011762.1"
/db_xref="GeneID:853161"
/db_xref="SGD:S000003478"
/translation="MPVCKNCHGTEFERDLSNANNDLVCKACGVVSEDNPIVSEVTFG
ETSAGAAVVQGSFIGAGQSHAAFGGSSALESREATLNNARRKLRAVSYALHIPEYITD
AAFQWYKLALANNFVQGRRSQNVIASCLYVACRKEKTHHMLIDFSSRLQVSVYSIGAT
FLKMVKKLHITELPLADPSLFIQHFAEKLDLADKKIKVVKDAVKLAQRMSKDWMFEGR
RPAGIAGACILLACRMNNLRRTHTEIVAVSHVAEETLQQRLNEFKNTKAAKLSVQKFR
ENDVEDGEARPPSFVKNRKKERKIKDSLDKEEMFQTSEEALNKNPILTQVLGEQELSS
KEVLFYLKQFSERRARVVERIKATNGIDGENIYHEGSENETRKRKLSEVSIQNEHVEG
EDKETEGTEEKVKKVKTKTSEEKKENESGHFQDAIDGYSLETDPYCPRNLHLLPTTDT
YLSKVSDDPDNLEDVDDEELNAHLLNEEASKLKERIWIGLNADFLLEQESKRLKQEAD
IATGNTSVKKKRTRRRNNTRSDEPTKTVDAAAAIGLMSDLQDKSGLHAALKAAEESGD
FTTADSVKNMLQKASFSKKINYDAIDGLFR"
gene <984966..>985685
/gene="CPD1"
/locus_tag="YGR247W"
/db_xref="GeneID:853162"
mRNA <984966..>985685
/gene="CPD1"
/locus_tag="YGR247W"
/product="2',3'-cyclic-nucleotide 3'-phosphodiesterase"
/transcript_id="NM_001181376.1"
/db_xref="GeneID:853162"
CDS 984966..985685
/gene="CPD1"
/locus_tag="YGR247W"
/EC_number="3.1.4.37"
/experiment="EXISTENCE:direct assay:GO:0004113
2',3'-cyclic-nucleotide 3'-phosphodiesterase activity
[PMID:10734185]"
/experiment="EXISTENCE:direct assay:GO:0009187 cyclic
nucleotide metabolic process [PMID:10734185]"
/experiment="EXISTENCE:direct assay:GO:0030136
clathrin-coated vesicle [PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0004113
2',3'-cyclic-nucleotide 3'-phosphodiesterase activity
[PMID:10734185]"
/note="Cyclic nucleotide phosphodiesterase; hydrolyzes
ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose
1''-phosphate; may have a role in tRNA splicing; no
detectable phenotype is conferred by null mutation or by
overexpression; protein abundance increases in response to
DNA replication stress"
/codon_start=1
/product="2',3'-cyclic-nucleotide 3'-phosphodiesterase"
/protein_id="NP_011763.1"
/db_xref="GeneID:853162"
/db_xref="SGD:S000003479"
/translation="MAIALWYCPPQGSVAYETLQMLIFSFQTLFPDSPVFEPHVTVTS
HLVCNSKDDVNKILTSCVAAIQSIRSHQTAKKGRKGQVSHAVAAPLVSFNGCSVGKQY
FKKIVLECNKNKILYGVAQVMREMYVEIDPETRSSRAATWVHEEFHPHVSLLYSDIHP
VSQASLRVVQQRIEDALDVQLVPREKRKGSGNADGSNEVQMRWDFDVSSSLSWNIPGT
FKVVNCVGPVQEWEVLGRVDV"
gene <985972..>986739
/gene="SOL4"
/locus_tag="YGR248W"
/db_xref="GeneID:853163"
mRNA <985972..>986739
/gene="SOL4"
/locus_tag="YGR248W"
/product="6-phosphogluconolactonase SOL4"
/transcript_id="NM_001181377.3"
/db_xref="GeneID:853163"
CDS 985972..986739
/gene="SOL4"
/locus_tag="YGR248W"
/EC_number="3.1.1.31"
/experiment="EXISTENCE:curator inference:GO:0009051
pentose-phosphate shunt, oxidative branch [PMID:15454531]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:15454531]"
/experiment="EXISTENCE:genetic interaction:GO:0017057
6-phosphogluconolactonase activity [PMID:15454531]"
/note="6-phosphogluconolactonase; protein abundance
increases in response to DNA replication stress; SOL4 has
a paralog, SOL3, that arose from the whole genome
duplication"
/codon_start=1
/product="6-phosphogluconolactonase SOL4"
/protein_id="NP_011764.3"
/db_xref="GeneID:853163"
/db_xref="SGD:S000003480"
/translation="MVKLQRFSEKKSLIHEFGKFILEKQESALTGDADAVFNIAISGG
SMNQALYESLVNDKNIFPHIKWPQWRIFFCDERLVPFEDPQSNYGQFKKTVLDPLVHQ
GNQLNLGPTVYTINESLIGGGETANRKIAEEYASMLPASFDLILLGCGEDGHTCSLFP
GVEFNYLVEEMDRKVLWCNNSPKAPKDRITFTLAVVAEAKSVCFLVRGAAKKAIMHDV
LIVKNSELPSVLVNEMVGTKVTWFLDDEAGALIPENC"
gene <988049..>989419
/gene="MGA1"
/locus_tag="YGR249W"
/db_xref="GeneID:853164"
mRNA <988049..>989419
/gene="MGA1"
/locus_tag="YGR249W"
/product="Mga1p"
/transcript_id="NM_001181378.1"
/db_xref="GeneID:853164"
CDS 988049..989419
/gene="MGA1"
/locus_tag="YGR249W"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:16449570]"
/experiment="EXISTENCE:genetic interaction:GO:0007124
pseudohyphal growth [PMID:9832522]"
/experiment="EXISTENCE:mutant phenotype:GO:0007124
pseudohyphal growth [PMID:9832522]"
/note="Protein similar to heat shock transcription factor;
multicopy suppressor of pseudohyphal growth defects of
ammonium permease mutants"
/codon_start=1
/product="Mga1p"
/protein_id="NP_011765.1"
/db_xref="GeneID:853164"
/db_xref="SGD:S000003481"
/translation="MQPKTFVHQLHAILLEPEVNKWIYWSPTDNTVFFLKPYDPNFST
HVLKRYFKHGNVNSFVRQLHMYGFHKLSHPSPDQSSANNGNVKELVEWKFTHPSGFFF
KEANAGILNKIQRKSTGVGKDGKRKNILSPISVSYVDASRLNVLSQQSGPVSAREPSN
MFMGSPVHYSTSQSPPHISIPQQQQSSGPYLISSLPPQQPTVNMMRRQSISARMMNSY
DYPNQFSTQDSIVQPQQPQQVLSPQALSGPPMKKSGTLSSTDDLKTTSLPIVNYPMPY
HPGAFAQQQQQQQQPLPTVPPYSSYSTPFPSMMNSLSNSASNSPALGVCNNNVTLPKK
SNISERQALDNHIQTLKNSLSTITDLIEKHINSASQDENKTLTNDAMNKDLRTSLSLL
QNSKEEIIQLESKWMSMQSVKTTALPLQETTNTSSTLTSLTSSIIPKSIPIITKGEVA
TKPASY"
gene complement(<991176..>993521)
/gene="RIE1"
/locus_tag="YGR250C"
/gene_synonym="WHI8"
/db_xref="GeneID:853165"
mRNA complement(<991176..>993521)
/gene="RIE1"
/locus_tag="YGR250C"
/gene_synonym="WHI8"
/product="Rie1p"
/transcript_id="NM_001181379.3"
/db_xref="GeneID:853165"
CDS complement(991176..993521)
/gene="RIE1"
/locus_tag="YGR250C"
/gene_synonym="WHI8"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405|PMID:18981231]"
/experiment="EXISTENCE:physical interaction:GO:2000134
negative regulation of G1/S transition of mitotic cell
cycle [PMID:33480968]"
/note="RNA binding protein and negative regulator of
START; binds mRNA of the G1 cyclin CLN3 to stress granules
for translational repression under conditions of stress;
recruits the cyclin-dependent protein kinase Cdc28p to
stress granules when stressful conditions are relieved,
resulting in the dissolution of the granules, and
resumption of translation; localizes to stress granules
induced by glucose deprivation; protein abundance
increases in response to DNA replication stress"
/codon_start=1
/product="Rie1p"
/protein_id="NP_011766.3"
/db_xref="GeneID:853165"
/db_xref="SGD:S000003482"
/translation="MNIAEEPSDEVISSGPEDTDICSQQTSASAEAGDQSIKIERKTS
TGLQLEQLANTNLLTIRIKWQLQEEEDDHCNSRITDQIMDTIQHYKGISVNNSDTETY
EFLPDTRRLQVLEQNKDIYLYEHGSQEYEKSYKDNEEEDDWRYDTVLQAQFKYPKSLE
NACTDISELLKSEPIGQHIDKWSIGVNKHALTYPGNIFVGGIAKSLSIGELSFLFSKY
GPILSMKLIYDKTKGEPNGYGFISYPLGSQASLCIKELNGRTVNGSTLFINYHVERKE
RERIHWDHVKENNNDDNFRCLFIGNLPYHNPEKVETLITPKEVIEVIKKELSKKFPDF
DIISYYFPKRSNTRSSSSVSFNEEGSVESNKSSNNTNGNAQDEDMLKGYGFIKLINHE
QALAAIETFNGFMWHGNRLVVNKAVQHKVYNNHNSHDRHPSISNHNDMEVLEFANNPM
YDYNNYTYDRYYFNNNKNGNSNDTSNVRYFDSVRSTPVAEKMDLFYPQRESFSEGRGQ
RVPRFMGNKFDMYQYPSTSYSLPIPMSNQQESNLYVKHIPLSWTDEDLYDFYKSFGEI
ISVKVITVGGSKNKYRQQSNDSSSDNDLPVGSSRGYGFVSFESPLDAAKAILNTDGYQ
VSKDQVLSVSFAQKRGNLSSSDDDDQSQTDNSSKFQNFQPHNDYHKAYPTKYNKKFIN
ALMTQNQSQQQVSRENYFIPLQYPNTNTKPVNSYNLISANQNNANWMMPMFPSFGFIP
QVPPVPYIIPPQNPAANHIPIMANGSNEEEEFSSGDYSMDY"
gene <995639..>996229
/gene="NOP19"
/locus_tag="YGR251W"
/db_xref="GeneID:853166"
mRNA <995639..>996229
/gene="NOP19"
/locus_tag="YGR251W"
/product="Nop19p"
/transcript_id="NM_001181380.3"
/db_xref="GeneID:853166"
CDS 995639..996229
/gene="NOP19"
/locus_tag="YGR251W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095|PMID:19159085|PMID:14690591]"
/experiment="EXISTENCE:direct assay:GO:0005654 nucleoplasm
[PMID:21941128]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:14690591|PMID:21941128|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:21941128]"
/experiment="EXISTENCE:mutant phenotype:GO:0000447
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from
5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:21941128]"
/experiment="EXISTENCE:mutant phenotype:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12837249]"
/experiment="EXISTENCE:mutant phenotype:GO:0000472
endonucleolytic cleavage to generate mature 5'-end of
SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:21941128]"
/experiment="EXISTENCE:mutant phenotype:GO:0000480
endonucleolytic cleavage in 5'-ETS of tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:21941128]"
/note="Ribosome biogenesis factor; nucleolar protein
associated with pre-rRNA components of the 90S
preribosome, required for cleavage of pre-rRNA at A0, A1
and A2 sites; interacts with RNA helicase Dhr2p and RNA
helicase-like protein Utp25p; required for incorporation
of Utp25p into preribosomes"
/codon_start=1
/product="Nop19p"
/protein_id="NP_011767.3"
/db_xref="GeneID:853166"
/db_xref="SGD:S000003483"
/translation="MSRAKELQEKLNLQAKLQSTFSNNTAAVLDWLKESDETGISNDT
ERNKQLKDHKELEDGKKAFFKLPVLQIGSGLHFRTQDDASAKEDIHTIGEFIEGDKKV
SSLAKKKKRSDPGLQRNNMYRITKDDTKAMIALKRKMRKGEKEGLRKKQEHSKSSVSN
SYSASDEEDEDAGTMPQKSTKKKFGLLFDKKKKARK"
gene <996869..>998188
/gene="GCN5"
/locus_tag="YGR252W"
/gene_synonym="AAS104; ADA4; KAT2; SWI9"
/db_xref="GeneID:853167"
mRNA <996869..>998188
/gene="GCN5"
/locus_tag="YGR252W"
/gene_synonym="AAS104; ADA4; KAT2; SWI9"
/product="histone acetyltransferase GCN5"
/transcript_id="NM_001181381.1"
/db_xref="GeneID:853167"
CDS 996869..998188
/gene="GCN5"
/locus_tag="YGR252W"
/gene_synonym="AAS104; ADA4; KAT2; SWI9"
/EC_number="2.3.1.48"
/experiment="EXISTENCE:direct assay:GO:0000124 SAGA
complex [PMID:9674426|PMID:9224714]"
/experiment="EXISTENCE:direct assay:GO:0000775 chromosome,
centromeric region [PMID:18039853]"
/experiment="EXISTENCE:direct assay:GO:0003712
transcription coregulator activity [PMID:31699900]"
/experiment="EXISTENCE:direct assay:GO:0004402 histone
acetyltransferase activity [PMID:8601308]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0006325 chromatin
organization [PMID:9674426]"
/experiment="EXISTENCE:direct assay:GO:0010484 histone H3
acetyltransferase activity [PMID:18458063]"
/experiment="EXISTENCE:direct assay:GO:0046695 SLIK
(SAGA-like) complex [PMID:12446794]"
/experiment="EXISTENCE:direct assay:GO:0140011 histone
H4K12ac reader activity [PMID:20126658]"
/experiment="EXISTENCE:direct assay:GO:0140046 histone
H4K16ac reader activity [PMID:20126658]"
/experiment="EXISTENCE:direct assay:GO:0140068 histone
crotonyltransferase activity [PMID:31699900]"
/experiment="EXISTENCE:direct assay:GO:0140129 histone
H3K56ac reader activity [PMID:20126658]"
/experiment="EXISTENCE:direct assay:GO:0140671 ADA complex
[PMID:10490601|PMID:7862114]"
/experiment="EXISTENCE:genetic interaction:GO:0010484
histone H3 acetyltransferase activity [PMID:18458063]"
/experiment="EXISTENCE:genetic interaction:GO:0032968
positive regulation of transcription elongation by RNA
polymerase II [PMID:19822662]"
/experiment="EXISTENCE:mutant phenotype:GO:0006338
chromatin remodeling [PMID:11867538]"
/experiment="EXISTENCE:mutant phenotype:GO:0010484 histone
H3 acetyltransferase activity [PMID:18458063]"
/experiment="EXISTENCE:mutant phenotype:GO:0032968
positive regulation of transcription elongation by RNA
polymerase II [PMID:19822662]"
/note="Catalytic subunit of ADA and SAGA histone
acetyltransferase complexes; modifies N-terminal lysines
on histones H2B and H3; acetylates Rsc4p, a subunit of the
RSC chromatin-remodeling complex, altering replication
stress tolerance; relocalizes to the cytosol in response
to hypoxia; mutant displays reduced transcription
elongation in the G-less-based run-on (GLRO) assay;
greater involvement in repression of RNAPII-dependent
transcription than in activation"
/codon_start=1
/product="histone acetyltransferase GCN5"
/protein_id="NP_011768.1"
/db_xref="GeneID:853167"
/db_xref="SGD:S000003484"
/translation="MVTKHQIEEDHLDGATTDPEVKRVKLENNVEEIQPEQAETNKQE
GTDKENKGKFEKETERIGGSEVVTDVEKGIVKFEFDGVEYTFKERPSVVEENEGKIEF
RVVNNDNTKENMMVLTGLKNIFQKQLPKMPKEYIARLVYDRSHLSMAVIRKPLTVVGG
ITYRPFDKREFAEIVFCAISSTEQVRGYGAHLMNHLKDYVRNTSNIKYFLTYADNYAI
GYFKKQGFTKEITLDKSIWMGYIKDYEGGTLMQCSMLPRIRYLDAGKILLLQEAALRR
KIRTISKSHIVRPGLEQFKDLNNIKPIDPMTIPGLKEAGWTPEMDALAQRPKRGPHDA
AIQNILTELQNHAAAWPFLQPVNKEEVPDYYDFIKEPMDLSTMEIKLESNKYQKMEDF
IYDARLVFNNCRMYNGENTSYYKYANRLEKFFNNKVKEIPEYSHLID"
gene complement(<998358..>999140)
/gene="PUP2"
/locus_tag="YGR253C"
/gene_synonym="DOA5"
/db_xref="GeneID:853168"
mRNA complement(<998358..>999140)
/gene="PUP2"
/locus_tag="YGR253C"
/gene_synonym="DOA5"
/product="proteasome core particle subunit alpha 5"
/transcript_id="NM_001181382.1"
/db_xref="GeneID:853168"
CDS complement(998358..999140)
/gene="PUP2"
/locus_tag="YGR253C"
/gene_synonym="DOA5"
/experiment="EXISTENCE:direct assay:GO:0010499 proteasomal
ubiquitin-independent protein catabolic process
[PMID:19162040]"
/experiment="EXISTENCE:direct assay:GO:0019773 proteasome
core complex, alpha-subunit complex [PMID:9087403]"
/experiment="EXISTENCE:direct assay:GO:0034515 proteasome
storage granule [PMID:18504300]"
/experiment="EXISTENCE:direct assay:GO:0043161
proteasome-mediated ubiquitin-dependent protein catabolic
process [PMID:19029916|PMID:11545745]"
/note="Alpha 5 subunit of the 20S proteasome; involved in
ubiquitin-dependent catabolism; human homolog is subunit
zeta"
/codon_start=1
/product="proteasome core particle subunit alpha 5"
/protein_id="NP_011769.1"
/db_xref="GeneID:853168"
/db_xref="SGD:S000003485"
/translation="MFLTRSEYDRGVSTFSPEGRLFQVEYSLEAIKLGSTAIGIATKE
GVVLGVEKRATSPLLESDSIEKIVEIDRHIGCAMSGLTADARSMIEHARTAAVTHNLY
YDEDINVESLTQSVCDLALRFGEGASGEERLMSRPFGVALLIAGHDADDGYQLFHAEP
SGTFYRYNAKAIGSGSEGAQAELLNEWHSSLTLKEAELLVLKILKQVMEEKLDENNAQ
LSCITKQDGFKIYDNEKTAELIKELKEKEAAESPEEADVEMS"
rep_origin 999456..999576
/note="ARS734; Autonomously Replicating Sequence"
/db_xref="SGD:S000118468"
gene <1000927..>1002240
/gene="ENO1"
/locus_tag="YGR254W"
/gene_synonym="HSP48"
/db_xref="GeneID:853169"
mRNA <1000927..>1002240
/gene="ENO1"
/locus_tag="YGR254W"
/gene_synonym="HSP48"
/product="phosphopyruvate hydratase ENO1"
/transcript_id="NM_001181383.3"
/db_xref="GeneID:853169"
CDS 1000927..1002240
/gene="ENO1"
/locus_tag="YGR254W"
/gene_synonym="HSP48"
/EC_number="4.2.1.11"
/experiment="EXISTENCE:direct assay:GO:0000015
phosphopyruvate hydratase complex [PMID:6282834]"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:16565073]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16962558]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836]"
/experiment="EXISTENCE:direct assay:GO:0032889 regulation
of vacuole fusion, non-autophagic [PMID:16565073]"
/experiment="EXISTENCE:direct assay:GO:1904408 melatonin
binding [PMID:31708896]"
/experiment="EXISTENCE:mutant phenotype:GO:0004634
phosphopyruvate hydratase activity [PMID:6282834]"
/experiment="EXISTENCE:mutant phenotype:GO:0006096
glycolytic process [PMID:6282834]"
/experiment="EXISTENCE:mutant phenotype:GO:0032889
regulation of vacuole fusion, non-autophagic
[PMID:16565073]"
/note="Enolase I, a phosphopyruvate hydratase; catalyzes
conversion of 2-phosphoglycerate to phosphoenolpyruvate
during glycolysis and the reverse reaction during
gluconeogenesis; expression repressed in response to
glucose; protein abundance increases in response to DNA
replication stress; N-terminally propionylated in vivo;
ENO1 has a paralog, ENO2, that arose from the whole genome
duplication"
/codon_start=1
/product="phosphopyruvate hydratase ENO1"
/protein_id="NP_011770.3"
/db_xref="GeneID:853169"
/db_xref="SGD:S000003486"
/translation="MAVSKVYARSVYDSRGNPTVEVELTTEKGVFRSIVPSGASTGVH
EALEMRDGDKSKWMGKGVLHAVKNVNDVIAPAFVKANIDVKDQKAVDDFLISLDGTAN
KSKLGANAILGVSLAASRAAAAEKNVPLYKHLADLSKSKTSPYVLPVPFLNVLNGGSH
AGGALALQEFMIAPTGAKTFAEALRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPN
IQTAEEALDLIVDAIKAAGHDGKIKIGLDCASSEFFKDGKYDLDFKNPNSDKSKWLTG
PQLADLYHSLMKRYPIVSIEDPFAEDDWEAWSHFFKTAGIQIVADDLTVTNPKRIATA
IEKKAADALLLKVNQIGTLSESIKAAQDSFAAGWGVMVSHRSGETEDTFIADLVVGLR
TGQIKTGAPARSERLAKLNQLLRIEEELGDNAVFAGENFHHGDKL"
rep_origin 1002239..1002522
/note="ARS735; Replication origin; identified in multiple
array studies, confirmed by plasmid-based recombinational
ARS assay"
/db_xref="SGD:S000130168"
gene complement(<1002523..>1003962)
/gene="COQ6"
/locus_tag="YGR255C"
/db_xref="GeneID:853170"
mRNA complement(<1002523..>1003962)
/gene="COQ6"
/locus_tag="YGR255C"
/product="putative N,N-dimethylaniline monooxygenase COQ6"
/transcript_id="NM_001181384.1"
/db_xref="GeneID:853170"
CDS complement(1002523..1003962)
/gene="COQ6"
/locus_tag="YGR255C"
/EC_number="1.14.15.45"
/EC_number="1.14.15.46"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:12721307]"
/experiment="EXISTENCE:mutant phenotype:GO:0006744
ubiquinone biosynthetic process
[PMID:21944752|PMID:9266513]"
/experiment="EXISTENCE:mutant phenotype:GO:0016712
oxidoreductase activity, acting on paired donors, with
incorporation or reduction of molecular oxygen, reduced
flavin or flavoprotein as one donor, and incorporation of
one atom of oxygen [PMID:21944752]"
/note="Flavin-dependent monooxygenase involved in
ubiquinone biosynthesis; responsible for hydroxylation at
position C5 and deamination at C4 during ubiquinone
(Coenzyme Q) biosynthesis; localizes to matrix face of
mitochondrial inner membrane in a large complex with other
ubiquinone biosynthetic enzymes; human homolog COQ6 can
complement yeast null mutant and is implicated in
steroid-resistant nephrotic syndrome (SRNS)"
/codon_start=1
/product="putative N,N-dimethylaniline monooxygenase COQ6"
/protein_id="NP_011771.1"
/db_xref="GeneID:853170"
/db_xref="SGD:S000003487"
/translation="MFFSKVMLTRRILVRGLATAKSSAPKLTDVLIVGGGPAGLTLAA
SIKNSPQLKDLKTTLVDMVDLKDKLSDFYNSPPDYFTNRIVSVTPRSIHFLENNAGAT
LMHDRIQSYDGLYVTDGCSKATLDLARDSMLCMIEIINIQASLYNRISQYDSKKDSID
IIDNTKVVNIKHSDPNDPLSWPLVTLSNGEVYKTRLLVGADGFNSPTRRFSQIPSRGW
MYNAYGVVASMKLEYPPFKLRGWQRFLPTGPIAHLPMPENNATLVWSSSERLSRLLLS
LPPESFTALINAAFVLEDADMNYYYRTLEDGSMDTDKLIEDIKFRTEEIYATLKDESD
IDEIYPPRVVSIIDKTRARFPLKLTHADRYCTDRVALVGDAAHTTHPLAGQGLNMGQT
DVHGLVYALEKAMERGLDIGSSLSLEPFWAERYPSNNVLLGMADKLFKLYHTNFPPVV
ALRTFGLNLTNKIGPVKNMIIDTLGGNEK"
gene 1004216..1004287
/locus_tag="YNCG0046W"
/db_xref="GeneID:853171"
tRNA 1004216..1004287
/locus_tag="YNCG0046W"
/product="tRNA-Thr"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0030371 translation
repressor activity [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043555 regulation
of translation in response to stress [PMID:27609601]"
/note="Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:853171"
/db_xref="SGD:S000006750"
gene <1004624..>1006102
/gene="GND2"
/locus_tag="YGR256W"
/db_xref="GeneID:853172"
mRNA <1004624..>1006102
/gene="GND2"
/locus_tag="YGR256W"
/product="phosphogluconate dehydrogenase (decarboxylating)
GND2"
/transcript_id="NM_001181385.3"
/db_xref="GeneID:853172"
CDS 1004624..1006102
/gene="GND2"
/locus_tag="YGR256W"
/EC_number="1.1.1.44"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836]"
/experiment="EXISTENCE:mutant phenotype:GO:0004616
phosphogluconate dehydrogenase (decarboxylating) activity
[PMID:1328471]"
/experiment="EXISTENCE:mutant phenotype:GO:0009051
pentose-phosphate shunt, oxidative branch [PMID:1328471]"
/note="6-phosphogluconate dehydrogenase (decarboxylating);
catalyzes an NADPH regenerating reaction in the pentose
phosphate pathway; required for growth on
D-glucono-delta-lactone; GND2 has a paralog, GND1, that
arose from the whole genome duplication"
/codon_start=1
/product="phosphogluconate dehydrogenase (decarboxylating)
GND2"
/protein_id="NP_011772.3"
/db_xref="GeneID:853172"
/db_xref="SGD:S000003488"
/translation="MSKAVGDLGLVGLAVMGQNLILNAADHGFTVVAYNRTQSKVDRF
LANEAKGKSIIGATSIEDLVAKLKKPRKIMLLIKAGAPVDTLIKELVPHLDKGDIIID
GGNSHFPDTNRRYEELTKQGILFVGSGVSGGEDGARFGPSLMPGGSAEAWPHIKNIFQ
SIAAKSNGEPCCEWVGPAGSGHYVKMVHNGIEYGDMQLICEAYDIMKRIGRFTDKEIS
EVFDKWNTGVLDSFLIEITRDILKFDDVDGKPLVEKIMDTAGQKGTGKWTAINALDLG
MPVTLIGEAVFARCLSAIKDERKRASKLLAGPTVPKDAIHDREQFVYDLEQALYASKI
ISYAQGFMLIREAARSYGWKLNNPAIALMWRGGCIIRSVFLAEITKAYRDDPDLENLL
FNEFFASAVTKAQSGWRRTIALAATYGIPTPAFSTALAFYDGYRSERLPANLLQAQRD
YFGAHTFRILPECASAHLPVDKDIHINWTGHGGNISSSTYQA"
gene complement(<1006205..>1007305)
/gene="MTM1"
/locus_tag="YGR257C"
/db_xref="GeneID:853173"
mRNA complement(<1006205..>1007305)
/gene="MTM1"
/locus_tag="YGR257C"
/product="Mtm1p"
/transcript_id="NM_001181386.3"
/db_xref="GeneID:853173"
CDS complement(1006205..1007305)
/gene="MTM1"
/locus_tag="YGR257C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:12890866]"
/experiment="EXISTENCE:direct assay:GO:0030170 pyridoxal
phosphate binding [PMID:24184947]"
/experiment="EXISTENCE:mutant phenotype:GO:0006879
intracellular iron ion homeostasis [PMID:16601688]"
/experiment="EXISTENCE:mutant phenotype:GO:0031921
pyridoxal phosphate transport [PMID:25637770]"
/note="Mitochondrial protein of the mitochondrial carrier
family; high affinity pyridoxal 5'-phosphate (PLP)
transporter, important for delivery of the PLP cofactor to
mitochondrial enzymes; involved in mitochondrial iron
homeostasis"
/codon_start=1
/product="Mtm1p"
/protein_id="NP_011773.3"
/db_xref="GeneID:853173"
/db_xref="SGD:S000003489"
/translation="MSDRNTSNSLTLKERMLSAGAGSVLTSLILTPMDVVRIRLQQQQ
MIPDCSCDGAAEVPNAVSSGSKMKTFTNVGGQNLNNAKIFWESACFQELHCKNSSLKF
NGTLEAFTKIASVEGITSLWRGISLTLLMAIPANMVYFSGYEYIRDVSPIASTYPTLN
PLFCGAIARVFAATSIAPLELVKTKLQSIPRSSKSTKTWMMVKDLLNETRQEMKMVGP
SRALFKGLEITLWRDVPFSAIYWSSYELCKERLWLDSTRFASKDANWVHFINSFASGC
ISGMIAAICTHPFDVGKTRWQISMMNNSDPKGGNRSRNMFKFLETIWRTEGLAALYTG
LAARVIKIRPSCAIMISSYEISKKVFGNKLHQ"
gene complement(<1007671..>1010766)
/gene="RAD2"
/locus_tag="YGR258C"
/db_xref="GeneID:853174"
mRNA complement(<1007671..>1010766)
/gene="RAD2"
/locus_tag="YGR258C"
/product="ssDNA endodeoxyribonuclease RAD2"
/transcript_id="NM_001181387.1"
/db_xref="GeneID:853174"
CDS complement(1007671..1010766)
/gene="RAD2"
/locus_tag="YGR258C"
/experiment="EXISTENCE:direct assay:GO:0000014
single-stranded DNA endodeoxyribonuclease activity
[PMID:8247134]"
/experiment="EXISTENCE:direct assay:GO:0000112
nucleotide-excision repair factor 3 complex
[PMID:8855246]"
/experiment="EXISTENCE:direct assay:GO:0006289
nucleotide-excision repair [PMID:7926735]"
/experiment="EXISTENCE:genetic interaction:GO:0006366
transcription by RNA polymerase II [PMID:12110180]"
/experiment="EXISTENCE:mutant phenotype:GO:0006289
nucleotide-excision repair [PMID:7926735]"
/note="Single-stranded DNA endonuclease; cleaves
single-stranded DNA during nucleotide excision repair to
excise damaged DNA; subunit of Nucleotide Excision Repair
Factor 3 (NEF3); homolog of human XPG protein"
/codon_start=1
/product="ssDNA endodeoxyribonuclease RAD2"
/protein_id="NP_011774.1"
/db_xref="GeneID:853174"
/db_xref="SGD:S000003490"
/translation="MGVHSFWDIAGPTARPVRLESLEDKRMAVDASIWIYQFLKAVRD
QEGNAVKNSHITGFFRRICKLLYFGIRPVFVFDGGVPVLKRETIRQRKERRQGKRESA
KSTARKLLALQLQNGSNDNVKNSTPSSGSSVQIFKPQDEWDLPDIPGFKYDKEDARVN
SNKTFEKLMNSINGDGLEDIDLDTINPASAEFEELPKATQYLILSSLRLKSRLRMGYS
KEQLETIFPNSMDFSRFQIDMVKRRNFFTQKLINTTGFQDGGASKLNEEVINRISGQK
SKEYKLTKTNNGWILGLGANDGSDAQKAIVIDDKDAGALVKQLDSNAEDGDVLRWDDL
EDNSLKIVRHESSNATTAPQKRSNRSEDEGCDSDECEWEEVELKPKNVKFVEDFSLKA
ARLPYMGQSLNNAGSKSFLDKRHDQASPSKTTPTMRISRISVEDDDEDYLKQIEEIEM
MEAVQLSKMEKKPEADDKSKIAKPVTSKGTEARPPIVQYGLLGAQPDSKQPYHVTNLN
SKSESVIKRTSKTVLSEFRPPSQQEDKGAILTEGEQNLNFISHKIPQFDFNNENSLLF
QKNTESNVSQEATKEKSPIPEMPSWFSSTASQQLYNPYNTTNFVEDKNVRNEQESGAE
TTNKGSSYELLTGLNATEILERESEKESSNDENKDDDLEVLSEELFEDVPTKSQISKE
AEDNDSRKVESINKEHRKPLIFDYDFSEDEEDNIVENMIKEQEEFDTFKNTTLSTSAE
RNVAENAFVEDELFEQQMKDKRDSDEVTMDMIKEVQELLSRFGIPYITAPMEAEAQCA
ELLQLNLVDGIITDDSDVFLFGGTKIYKNMFHEKNYVEFYDAESILKLLGLDRKNMIE
LAQLLGSDYTNGLKGMGPVSSIEVIAEFGNLKNFKDWYNNGQFDKRKQETENKFEKDL
RKKLVNNEIILDDDFPSVMVYDAYMRPEVDHDTTPFVWGVPDLDMLRSFMKTQLGWPH
EKSDEILIPLIRDVNKRKKKGKQKRINEFFPREYISGDKKLNTSKRISTATGKLKKRK
M"
misc_feature 1010927..1010949
/note="ETC4; Chromosome-organizing-clamp; tethers
chromosomal regions to the nuclear periphery; binds TFIIIC
transcription factor but does not recruit RNA Polymerase
III; can act as a transcription-blocking insulator or as a
heterochromatin barrier element; remains predominantly
localized to the nuclear periphery throughout cell cycle;
located between divergently transcribed genes RAD2 and
TNA1"
/db_xref="SGD:S000077075"
gene <1012485..>1014089
/gene="TNA1"
/locus_tag="YGR260W"
/db_xref="GeneID:853175"
mRNA <1012485..>1014089
/gene="TNA1"
/locus_tag="YGR260W"
/product="Tna1p"
/transcript_id="NM_001181389.1"
/db_xref="GeneID:853175"
CDS 1012485..1014089
/gene="TNA1"
/locus_tag="YGR260W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:34498791]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:34498791]"
/experiment="EXISTENCE:direct assay:GO:0032541 cortical
endoplasmic reticulum [PMID:34498791]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0046942
carboxylic acid transport [PMID:10869563]"
/experiment="EXISTENCE:mutant phenotype:GO:0046943
carboxylic acid transmembrane transporter activity
[PMID:10869563]"
/experiment="EXISTENCE:mutant phenotype:GO:1903222
quinolinic acid transmembrane transport [PMID:23457190]"
/note="High affinity nicotinic acid plasma membrane
permease; responsible for uptake of low levels of
nicotinic acid; expression of the gene increases in the
absence of extracellular nicotinic acid or
para-aminobenzoate (PABA)"
/codon_start=1
/product="Tna1p"
/protein_id="NP_011776.1"
/db_xref="GeneID:853175"
/db_xref="SGD:S000003492"
/translation="MSNKFTMESPKHLVDDVLFISPTNDGSEEKPTEVTFQEDEGHDA
SLHNRSHDKKSELATEREIMATTTDDDGIPSPSHPMEKRVLRKMDIYLIPLMGMLYFL
SNLDKSNIGNAEVAGLSKDIHLVGTQYNTCVTVFFATYVLFDPIGTNLLKIMGPPLMM
SICLTCFGAISLGTAWVKNYAQLIVVRLLLGAFEGMIYPAINMYLSVCYRREQYALRF
AFVFSAACLSSSFGGLIAYGCSKISGSLKDWQYIYIVEGCISLGFVPFYAFGLSKNLE
DSWFFNKEEKEYISERYKTMNTFDPDEKFEWFQVWQAVKDVKTWASAVALFGIDLTTF
GLTVFLPIIITSMGFTNVRAQLMTVPIYFLTAIVFFICAVWSDRIKLRSPFILGACLT
TSIGIAIVLGSQVHGVRYFGVYILCMGIYVNAACNCLWLSGNTGNYFKRATALGINLF
FGSGSGLVSGQIFVAKDKPRYIKGLSISLAFQVFSIFMTVVQIFLYKRENDKKKAIID
RCNELGEPIPYDERLSDKNPEFKYMY"
gene complement(<1014321..>1016750)
/gene="APL6"
/locus_tag="YGR261C"
/gene_synonym="YKS5"
/db_xref="GeneID:853177"
mRNA complement(<1014321..>1016750)
/gene="APL6"
/locus_tag="YGR261C"
/gene_synonym="YKS5"
/product="AP-3 complex subunit beta"
/transcript_id="NM_001181390.3"
/db_xref="GeneID:853177"
CDS complement(1014321..1016750)
/gene="APL6"
/locus_tag="YGR261C"
/gene_synonym="YKS5"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0006623 protein
targeting to vacuole [PMID:17895371]"
/experiment="EXISTENCE:mutant phenotype:GO:0006896 Golgi
to vacuole transport [PMID:9335339]"
/experiment="EXISTENCE:mutant phenotype:GO:0030123 AP-3
adaptor complex [PMID:9250663]"
/note="Beta3-like subunit of the yeast AP-3 complex;
functions in transport of alkaline phosphatase to the
vacuole via the alternate pathway; exists in both
cytosolic and peripherally associated membrane-bound
pools"
/codon_start=1
/product="AP-3 complex subunit beta"
/protein_id="NP_011777.3"
/db_xref="GeneID:853177"
/db_xref="SGD:S000003493"
/translation="MVDSIHRIASALDTAKVITREAAAVATSKLGESSYTYYSQNINP
QQLVTLLNSRNSREVRDAMKRIISIMASDDDSIDVQLYFADVVKNITTNDTKVKRLIH
LYLLRFAENDPNLTLLSINSLQKSLSDSNSELRCFALSALSDMKMSSLAPIILHTVKK
LVTDPSAMVRGEVALAIIKLYRAGKNDYHEELLDILKELMADTDPKVISCAVLAYKEC
YADHLELLHGHFRRYCRIIKQLDSWSQSYLIELLIKYCKQYLPKPTVVDKSSEGSPRS
CPLPDKYNEIEYPSYEVVNDPDLDLFLQSLNCLIYSSNPTVILSCCNALYQLASPLQM
KNTKFIEALVRTVTMTENQGNKEMLLQAIHFLSILDQTLFLPYTKKFYVFPKDPIVAS
IWKIQILSTLINESNVKEIFKELKYYVASAHFPENVVIMAVKSLSRCGQLSTSWESHV
MKWLIDHMESHNLSASVLDAYVNVIRMLVQKNPTKHLRIIFKLADLLTVQTSLADNAR
AGIVWLFGEIASIEFKICPDVLRRLIQNFSNEGPETRCQILVLSAKLLSYDIDNFKQA
QVTGSEENNQNPPYYDFSGSRISQMYNAVLYLAKYDDEFDIRDRARMISSLFDSGKYE
IVSLLLQAPKPTARSDDFIVSARLETHTPEIKEFFRMLPWNTEITEVGETGNDIREGA
ELKDYNKYKKSFSSQSFITNNSARSFTSSSNAKLTGINDGDSNSISGKGNVNTFTSQN
GKKYRLQSLDEFFSDIPERKSKPRKIIKVVEESSDEDEDESEESSDDDEYSDSSLGTS
SSGTSSSHLEL"
gene complement(<1016974..>1017759)
/gene="BUD32"
/locus_tag="YGR262C"
/gene_synonym="LDB14"
/db_xref="GeneID:853178"
mRNA complement(<1016974..>1017759)
/gene="BUD32"
/locus_tag="YGR262C"
/gene_synonym="LDB14"
/product="serine/threonine protein kinase BUD32"
/transcript_id="NM_001181391.1"
/db_xref="GeneID:853178"
CDS complement(1016974..1017759)
/gene="BUD32"
/locus_tag="YGR262C"
/gene_synonym="LDB14"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0000408 EKC/KEOPS
complex [PMID:16874308|PMID:16564010]"
/experiment="EXISTENCE:direct assay:GO:0004674 protein
serine/threonine kinase activity
[PMID:14519092|PMID:9305753]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14519092|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:36416748]"
/experiment="EXISTENCE:genetic interaction:GO:0000722
telomere maintenance via recombination [PMID:23390378]"
/experiment="EXISTENCE:genetic interaction:GO:0000723
telomere maintenance [PMID:16564010]"
/experiment="EXISTENCE:mutant phenotype:GO:0000282
cellular bud site selection [PMID:11452010]"
/experiment="EXISTENCE:mutant phenotype:GO:0000722
telomere maintenance via recombination [PMID:23390378]"
/experiment="EXISTENCE:mutant phenotype:GO:0000723
telomere maintenance [PMID:36416748|PMID:16564010]"
/experiment="EXISTENCE:mutant phenotype:GO:0004674 protein
serine/threonine kinase activity [PMID:14519092]"
/experiment="EXISTENCE:mutant phenotype:GO:0016887 ATP
hydrolysis activity [PMID:36416748]"
/experiment="EXISTENCE:mutant phenotype:GO:0070525 tRNA
threonylcarbamoyladenosine metabolic process
[PMID:21183954]"
/experiment="EXISTENCE:physical interaction:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:16874308]"
/note="Component of KEOPS complex with Kae1p, Cgi121p,
Pcc1p, and Gon7p; provides the ATPase and GTPase
activities of the complex; EKC/KEOPS complex is required
for t6A tRNA modification and telomeric TG1-3
recombination; may have role in transcription; mutation is
functionally complemented by human ortholog TP53RK"
/codon_start=1
/product="serine/threonine protein kinase BUD32"
/protein_id="NP_011778.1"
/db_xref="GeneID:853178"
/db_xref="SGD:S000003494"
/translation="MTQEFIDKVSSYLTPDVDIAPISQGAEAIVFTTTTHPYLPRAKD
SHQKYIIKYRPPKRYRHPQIDQALTKHRTLNESRLLAKLYLIPGLCVPQLIACDPYNG
FIWLEFLGEDLPGGHGFSNLKNFLWMHDQDPYSDLVATTLRKVGRQIGLLHWNDYCHG
DLTSSNIVLVRDGARWTPHLIDFGLGSVSNLVEDKGVDLYVLERAILSTHSKHAEKYN
AWIMEGFEEVYREQGAKGAKKLKEVTKRFEEVRLRGRKRSMLG"
gene complement(<1017967..>1019241)
/gene="SAY1"
/locus_tag="YGR263C"
/db_xref="GeneID:853179"
mRNA complement(<1017967..>1019241)
/gene="SAY1"
/locus_tag="YGR263C"
/product="steryl deacetylase"
/transcript_id="NM_001181392.3"
/db_xref="GeneID:853179"
CDS complement(1017967..1019241)
/gene="SAY1"
/locus_tag="YGR263C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:24868093|PMID:26928762|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005788 endoplasmic
reticulum lumen [PMID:18034159]"
/experiment="EXISTENCE:direct assay:GO:0005811 lipid
droplet [PMID:24868093]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:18034159]"
/experiment="EXISTENCE:direct assay:GO:0034084 steryl
deacetylase activity [PMID:18034159]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0009636
response to toxic substance [PMID:18034159]"
/experiment="EXISTENCE:mutant phenotype:GO:0016125 sterol
metabolic process [PMID:18034159]"
/experiment="EXISTENCE:mutant phenotype:GO:0034084 steryl
deacetylase activity [PMID:18034159]"
/note="Sterol deacetylase; component of the sterol
acetylation/deacetylation cycle along with Atf2p; active
both in the endoplasmic reticulum (ER) and in lipid
droplets; integral membrane protein with active site in
the ER lumen; green fluorescent protein (GFP)-fusion
protein localizes to the ER"
/codon_start=1
/product="steryl deacetylase"
/protein_id="NP_011779.3"
/db_xref="GeneID:853179"
/db_xref="SGD:S000003495"
/translation="MAANSGLDSKVEYYRLQENEIISAVSSEDADQNDAGFRLSTIHL
HLFHGLKFAALLFTVVPVFIILDSMKIIFQRKRRFCLDHVNRSFLRQSSWILDERICQ
YVLNPLFVCLYPSTFSSPTYVKCNIPIEDQKSPENNIFQDHQLNAPKIVSTKFYQYVM
PEGFDPTTDPVLVFYHGGGYALKLTPTSFSFLNNMRNAFPKMAILVPDYTVTATDDQS
KKYPLQILQNVAIFDYVVKTMGCKNVVIMGDSAGGNAVLNIVLYLRKCHREIYPKKVI
AISPWANATFFHEGEKEYMQGTQEWDGLCLKSHSMFGRMFVGNNPNVDFTSDPFVNIE
KNFETKMWQDILKKCSVMITYGSDELLSFQNKILAKKMSDASEGCNHFTAKNVLVEHQ
GYHTGPILNYSRNMDRWTNIPSIARILEFMQS"
gene complement(<1019598..>1021853)
/gene="MES1"
/locus_tag="YGR264C"
/db_xref="GeneID:853181"
mRNA complement(<1019598..>1021853)
/gene="MES1"
/locus_tag="YGR264C"
/product="methionine--tRNA ligase MES1"
/transcript_id="NM_001181393.3"
/db_xref="GeneID:853181"
CDS complement(1019598..1021853)
/gene="MES1"
/locus_tag="YGR264C"
/EC_number="6.1.1.10"
/experiment="EXISTENCE:direct assay:GO:0004825
methionine-tRNA ligase activity [PMID:3312199]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:3905796]"
/experiment="EXISTENCE:direct assay:GO:0006431
methionyl-tRNA aminoacylation [PMID:3312199]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26359986]"
/experiment="EXISTENCE:direct assay:GO:0017102 methionyl
glutamyl tRNA synthetase complex [PMID:11069915]"
/experiment="EXISTENCE:direct assay:GO:1990825
sequence-specific mRNA binding [PMID:34039240]"
/note="Methionyl-tRNA synthetase; forms a complex with
Gus1p, a glutamyl-tRNA synthetase, and Arc1p, which
increases the catalytic efficiency of both synthetases;
involved in the nuclear export of tRNAs; mutations in
human ortholog MARS is associated with pediatric pulmonary
alveolar proteinosis, interstitial lung and liver disease,
and Charcot-Marie-Tooth disease; human MARS gene can
complement the yeast null mutant"
/codon_start=1
/product="methionine--tRNA ligase MES1"
/protein_id="NP_011780.3"
/db_xref="GeneID:853181"
/db_xref="SGD:S000003496"
/translation="MSFLISFDKSKKHPAHLQLANNLKIALALEYASKNLKPEVDNDN
AAMELRNTKEPFLLFDANAILRYVMDDFEGQTSDKYQFALASLQNLLYHKELPQQHVE
VLTNKAIENYLVELKEPLTTTDLILFANVYALNSSLVHSKFPELPSKVHNAVALAKKH
VPRDSSSFKNIGAVKIQADLTVKPKDSEILPKPNERNILITSALPYVNNVPHLGNIIG
SVLSADIFARYCKGRNYNALFICGTDEYGTATETKALEEGVTPRQLCDKYHKIHSDVY
KWFQIGFDYFGRTTTDKQTEIAQHIFTKLNSNGYLEEQSMKQLYCPVHNSYLADRYVE
GECPKCHYDDARGDQCDKCGALLDPFELINPRCKLDDASPEPKYSDHIFLSLDKLESQ
ISEWVEKASEEGNWSKNSKTITQSWLKDGLKPRCITRDLVWGTPVPLEKYKDKVLYVW
FDATIGYVSITSNYTKEWKQWWNNPEHVSLYQFMGKDNVPFHTVVFPGSQLGTEENWT
MLHHLNTTEYLQYENGKFSKSRGVGVFGNNAQDSGISPSVWRYYLASVRPESSDSHFS
WDDFVARNNSELLANLGNFVNRLIKFVNAKYNGVVPKFDPKKVSNYDGLVKDINEILS
NYVKEMELGHERRGLEIAMSLSARGNQFLQENKLDNTLFSQSPEKSDAVVAVGLNIIY
AVSSIITPYMPEIGEKINKMLNAPALKIDDRFHLAILEGHNINKAEYLFQRIDEKKID
EWRAKYGGQQV"
gene <1022656..>1024761
/locus_tag="YGR266W"
/db_xref="GeneID:853182"
mRNA <1022656..>1024761
/locus_tag="YGR266W"
/product="uncharacterized protein"
/transcript_id="NM_001181395.1"
/db_xref="GeneID:853182"
CDS 1022656..1024761
/locus_tag="YGR266W"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:16407407]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:12469340]"
/note="hypothetical protein; predicted to contain a single
transmembrane domain; mutant has increased aneuploidy
tolerance; localized to both the mitochondrial outer
membrane and the plasma membrane; protein abundance
increases in response to DNA replication stress"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011782.1"
/db_xref="GeneID:853182"
/db_xref="SGD:S000003498"
/translation="MHATNWFDDWNPEALYRDDVTGCDDCSETSPIPKSGIICGPILR
LINMDFKEKTYEGSIMVVVRGEENFPKITYQLGPSLPSEDEDIEVNEAFFEGKLFHKD
ILKDDNIWFYRYEIKLPMSNYEQMVKYAVDGTMEPHYRFFVPSFTQNSNVISYSCNGF
SLSVDTSKFKGSLWYDVLKKHRYVHYHAILGGGDQIYSDNIKLHAPNLKAWLETKDPI
KKYNTQTTEETKEQIRQFYLEHYLNWYGYGHWYGSTPKSKTTQKCFVKSLACIPAINV
WDDHDIIDGYGSYNDSFMKTENFLTVGRMAYRYYMLFQQHVSASKQDGDEYAYLKSKQ
WILGNEKGSSYIGERSHSIFSWLGPKMAMLGLDCRTERKLHEIFSERSYSLIWERVER
EIKNLKGGHLLLMLGIPIAYPRLVWLEWLFTSKLLAPIKYLSKKGIFASGFVNEFNGD
VELLDDLNDHWCARHHKKERNYLIMKLQDIGAKYGVRITILSGDVHLASVGRFRAKIH
RHHLIMSEEKEKENTRIIEEPTKDVRLIFNIIASAIVNTPPPDAMATLLQKRCRLHHF
DLETDEDAVPIFAKEVDGVHKRKESCFMNKRNWSDIIPIENLLNNPQLSKELGVKVGD
IVIPGIITEQQKLQKLENDDQINSYPVTSGGLFTTIHVERDANQTNSQTVSYCLPIPE
LTVTCERLSHKGIKHLNIT"
gene complement(<1025004..>1025735)
/gene="FOL2"
/locus_tag="YGR267C"
/db_xref="GeneID:853183"
mRNA complement(<1025004..>1025735)
/gene="FOL2"
/locus_tag="YGR267C"
/product="GTP cyclohydrolase I"
/transcript_id="NM_001181396.1"
/db_xref="GeneID:853183"
CDS complement(1025004..1025735)
/gene="FOL2"
/locus_tag="YGR267C"
/EC_number="3.5.4.16"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095|PMID:31323700]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276|PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0003934 GTP
cyclohydrolase I activity [PMID:8573145]"
/experiment="EXISTENCE:mutant phenotype:GO:0009396 folic
acid-containing compound biosynthetic process
[PMID:8573145]"
/note="GTP-cyclohydrolase I, catalyzes first step in folic
acid biosynthesis; human homolog GCH1 is implicated in
dopa-responsive dystonia (DRD), and can complement yeast
null mutant"
/codon_start=1
/product="GTP cyclohydrolase I"
/protein_id="NP_011783.1"
/db_xref="GeneID:853183"
/db_xref="SGD:S000003499"
/translation="MHNIQLVQEIERHETPLNIRPTSPYTLNPPVERDGFSWPSVGTR
QRAEETEEEEKERIQRISGAIKTILTELGEDVNREGLLDTPQRYAKAMLYFTKGYQTN
IMDDVIKNAVFEEDHDEMVIVRDIEIYSLCEHHLVPFFGKVHIGYIPNKKVIGLSKLA
RLAEMYARRLQVQERLTKQIAMALSDILKPLGVAVVMEASHMCMVSRGIQKTGSSTVT
SCMLGGFRAHKTREEFLTLLGRRSI"
gene complement(<1026057..>1026653)
/gene="HUA1"
/locus_tag="YGR268C"
/db_xref="GeneID:853185"
mRNA complement(<1026057..>1026653)
/gene="HUA1"
/locus_tag="YGR268C"
/product="Hua1p"
/transcript_id="NM_001181397.3"
/db_xref="GeneID:853185"
CDS complement(1026057..1026653)
/gene="HUA1"
/locus_tag="YGR268C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11489916|PMID:11914276]"
/note="Cytoplasmic protein containing a zinc finger
domain; sequence similarity to that of Type I J-proteins;
computational analysis of large-scale protein-protein
interaction data suggests a possible role in actin patch
assembly"
/codon_start=1
/product="Hua1p"
/protein_id="NP_011784.3"
/db_xref="GeneID:853185"
/db_xref="SGD:S000003500"
/translation="MSKDTHDDELPSYEDVIKEEERLQSQPPRPPRPAANLAQGHQSR
PHQRPSTMPATSSSQTYAHSHSYTPTSSQPRPPPRPQQNPSLPWTYPPRFYCSKCGNT
GYKLKNGRSCKSCWRRFAPQNNVVSAPTYYTNYTMPVYTNAWQGNRPLYVQPGDPRLG
GVLCGECRGSGRTRFLLDEDICPLCHGVGRIITQPQRY"
gene <1027370..>1031509
/gene="YTA7"
/locus_tag="YGR270W"
/db_xref="GeneID:853186"
mRNA <1027370..>1031509
/gene="YTA7"
/locus_tag="YGR270W"
/product="chromatin segregase YTA7"
/transcript_id="NM_001181399.1"
/db_xref="GeneID:853186"
CDS 1027370..1031509
/gene="YTA7"
/locus_tag="YGR270W"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:34471130]"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:18493054|PMID:19581291]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922|PMID:14562095|PMID:26928762|PMID:22932476|P
MID:19416104]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0006261
DNA-templated DNA replication [PMID:34471130]"
/experiment="EXISTENCE:direct assay:GO:0006337 nucleosome
disassembly [PMID:34471130]"
/experiment="EXISTENCE:direct assay:GO:0042393 histone
binding [PMID:19581291|PMID:16079223]"
/experiment="EXISTENCE:direct assay:GO:0140658
ATP-dependent chromatin remodeler activity
[PMID:34471130]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:18493054]"
/experiment="EXISTENCE:mutant phenotype:GO:0003682
chromatin binding [PMID:19581291]"
/experiment="EXISTENCE:mutant phenotype:GO:0006261
DNA-templated DNA replication [PMID:34471130]"
/experiment="EXISTENCE:mutant phenotype:GO:0006337
nucleosome disassembly [PMID:34471130]"
/experiment="EXISTENCE:mutant phenotype:GO:0016887 ATP
hydrolysis activity [PMID:32079723]"
/experiment="EXISTENCE:mutant phenotype:GO:0042393 histone
binding [PMID:19581291]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:19683497]"
/experiment="EXISTENCE:mutant phenotype:GO:0140658
ATP-dependent chromatin remodeler activity
[PMID:34471130]"
/experiment="EXISTENCE:mutant phenotype:GO:2000219
positive regulation of invasive growth in response to
glucose limitation [PMID:18202364]"
/note="Chromatin segregase involved in DNA replication;
ATPase activity, stimulated by S phase CDK-dependent
phosphorylation, is coupled to nucleosome disassembly,
promoting DNA replication; regulates cell-cycle-dependent
histone gene expression; contains AAA+-ATPase domains and
a non-canonical bromodomain; hexameric; relocalizes to the
cytosol in response to hypoxia; human ortholog ATAD2 is a
coregulator of oncogenic transcription factors and an
oncogene overexpressed in cancers with poor prognosis"
/codon_start=1
/product="chromatin segregase YTA7"
/protein_id="NP_011786.1"
/db_xref="GeneID:853186"
/db_xref="SGD:S000003502"
/translation="MARNLRNRRGSDVEDASNAKVGYETQIKDENGIIHTTTRSLRKI
NYAEIEKVFDFLEDDQVMDKDETPVDVTSDEHHNNNQKGDDEDDDVDLVSPHENARTN
EELTNERNLRKRKAHDPEEDDESFHEEDVDDDEEEEEADEFEDEYLDEDSKDNNRRRR
AADRKFVVPDPDDDEEYDEDDEEGDRISHSASSKRLKRANSRRTRSSRHPETPPPVRR
ALRSRTRHSRTSNEENDDENDNSRNEALTLADEIRELQEDSPIREKRFLRERTKPVNY
KLPPPLTASNAEEFIDKNNNALSFHNPSPARRGRGGWNASQNSGPTRRLFPTGGPFGG
NDVTTIFGKNTNFYNQVPSAFSDNNNNKLILDSDSSDDEILPLGVTPKTKKENTQKKK
KKKPEIADLDPLGVDMNVNFDDIGGLDNYIDQLKEMVALPLLYPELYQNFNITPPRGV
LFHGPPGTGKTLMARALAASCSSDERKITFFMRKGADILSKWVGEAERQLRLLFEEAK
KHQPSIIFFDEIDGLAPVRSSKQEQIHASIVSTLLALMDGMDNRGQVIVIGATNRPDA
VDPALRRPGRFDREFYFPLPDVKARFKILQIQTRKWSSPLSTNFIDKLAFLTKGYGGA
DLRSLCTEAALISIQRSFPQIYRSNDKLLVDPSKIKVKVSDFMLALKKIVPSSARSTG
SSPQPLPELIKPLLADQLNNLKNKLDYMLNIKDTTFQRNTSLLQNFIDYEEYSGEEEE
HDKYGGNEDTSSFRSYEFFESMAESQICKPRLLINGPKGNGQQYVGAAILNYLEEFNV
QNLDLASLVSESSRTIEAAVVQSFMEAKKRQPSVVFIPNLDIWINTIPENVILVLSGL
FRSLQSNEKILLLCLAENLDISEVKNGILSDFAFDKNIFQLHKPSKENITRYFSNLIE
LLKTKPSDIPMKKRRVKPLPELQKVTSNAAPTNFDENGEPLSEKVVLRRKLKSFQHQD
MRLKNVLKIKLSGLMDLFKNRYKRFRKPPIDDAFLVHLFEPETSNDPNWQPAYIKDEN
MILEVSTGRKFFNMDLDIVEERLWNGYYSEPKQFLKDIELIYRDANTIGDRERVIKAS
EMFANAQMGIEEISTPDFIQECKATRQRDLERQELFLEDEEKRAAMELEAKEQSQENI
LQEPDLKDNKANEFGVAAGNQLQAQLQTTINTASIVNNSEVPQPIDTNLYKKEIPAAI
PSAVDKEKAVIPEDSGANEEYTTELIQATCTSEITTDDDERARKEPKENEDSLQTQVT
EENFSKIDANTNNINHVKEIQSVNKPNSLHETVEKRERSPIPKEVVEPEQGKKSDKEL
ILTPEQIKKVSACLIEHCQNFTVSQLEDVHSSVAKIIWKSKSAWDKTGTVDEIIKFLS
E"
gene <1031791..>1037694
/gene="SLH1"
/locus_tag="YGR271W"
/gene_synonym="RQT2"
/db_xref="GeneID:853187"
mRNA <1031791..>1037694
/gene="SLH1"
/locus_tag="YGR271W"
/gene_synonym="RQT2"
/product="RNA helicase"
/transcript_id="NM_001181400.4"
/db_xref="GeneID:853187"
CDS 1031791..1037694
/gene="SLH1"
/locus_tag="YGR271W"
/gene_synonym="RQT2"
/EC_number="3.6.4.13"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:30893611]"
/experiment="EXISTENCE:direct assay:GO:0022626 cytosolic
ribosome [PMID:30893611]"
/experiment="EXISTENCE:direct assay:GO:0032790 ribosome
disassembly [PMID:30893611]"
/experiment="EXISTENCE:direct assay:GO:0072344 rescue of
stalled cytosolic ribosome [PMID:30893611]"
/experiment="EXISTENCE:genetic interaction:GO:0002181
cytoplasmic translation [PMID:21076151]"
/experiment="EXISTENCE:mutant phenotype:GO:0006417
regulation of translation [PMID:10922069|PMID:11438647]"
/note="Subunit of ribosome-associated quality control
trigger complex (RQT); contains ATPase activity required
for subunit dissociation of non-functional 80S ribosomes
containing polyubiquitinated Rps3p, leading to
Xrn1-dependent degradation of the non-functional 18S rRNA;
blocks translation of non-poly(A) mRNAs; required to
repress the propagation of dsRNA viruses; RNA
helicase-family protein related to Ski2p; human homolog is
ASCC3"
/codon_start=1
/product="RNA helicase"
/protein_id="NP_011787.4"
/db_xref="GeneID:853187"
/db_xref="SGD:S000003503"
/translation="MSTEYSADSSKSFMIAMQSMIDTSQTFNLDRSKISLPDFDDELK
KVQKDEQNQRTELTVLSQDRNDWDDIFEEFKDISFAQLQSIIDSYKTKNAVAVYKKIG
KLINEAETTLSSNVLLETVLQMVYKHQKQELEKELLDFLGTGNIDLVSLLLQHRRMIV
ATPIETTILLIKNAVNSTPEFLTQQDIRNQVLKSAEDAKNRKLNPATKIIKYPHVFRK
YEAGSTTAMAFAGQKFTLPVGTTRMSYNTHEEIIIPAADQASNKNYLYTKLLKISDLD
HFCKTVFPYETLNQIQSLVYPVAYKTNENMLICAPTGAGKTDIALLTIINTIKQFSVV
NGENEIDIQYDDFKVIYVAPLKALAAEIVDKFSKKLAPFNIQVRELTGDMQLTKAEIL
ATQVIVTTPEKWDVVTRKANGDNDLVSKVKLLIIDEVHLLHEDRGSVIETLVARTLRQ
VESSQSMIRIIGLSATLPNFMDVADFLGVNRQIGMFYFDQSFRPKPLEQQLLGCRGKA
GSRQSKENIDKVAYDKLSEMIQRGYQVMVFVHSRKETVKSARNFIKLAESNHEVDLFA
PDPIEKDKYSRSLVKNRDKDMKEIFQFGFGIHHAGMARSDRNLTEKMFKDGAIKVLCC
TATLAWGVNLPADCVIIKGTQVYDSKKGGFIDLGISDVIQIFGRGGRPGFGSANGTGI
LCTSNDRLDHYVSLITQQHPIESRFGSKLVDNLNAEISLGSVTNVDEAIEWLGYTYMF
VRMRKNPFTYGIDWEEIANDPQLYERRRKMIVVAARRLHALQMIVFDEVSMHFIAKDL
GRVSSDFYLLNESVEIFNQMCDPRATEADVLSMISMSSEFDGIKFREEESKELKRLSD
ESVECQIGSQLDTPQGKANVLLQAYISQTRIFDSALSSDSNYVAQNSVRICRALFLIG
VNRRWGKFSNVMLNICKSIEKRLWAFDHPLCQFDLPENIIRRIRDTKPSMEHLLELEA
DELGELVHNKKAGSRLYKILSRFPKINIEAEIFPITTNVMRIHIALGPDFVWDSRIHG
DAQFFWVFVEESDKSQILHFEKFILNRRQLNNQHEMDFMIPLSDPLPPQVVVKVVSDT
WIGCESTHAISFQHLIRPFNETLQTKLLKLRPLPTSALQNPLIESIYPFKYFNPMQTM
TFYTLYNTNENAFVGSPTGSGKTIVAELAIWHAFKTFPGKKIVYIAPMKALVRERVDD
WRKKITPVTGDKVVELTGDSLPDPKDVHDATIVITTPEKFDGISRNWQTRKFVQDVSL
IIMDEIHLLASDRGPILEMIVSRMNYISSQTKQPVRLLGMSTAVSNAYDMAGWLGVKD
HGLYNFPSSVRPVPLKMYIDGFPDNLAFCPLMKTMNKPVFMAIKQHSPDKPALIFVAS
RRQTRLTALDLIHLCGMEDNPRRFLNIDDEEELQYYLSQVTDDTLKLSLQFGIGLHHA
GLVQKDRSISHQLFQKNKIQILIATSTLAWGVNLPAHLVIIKGTQFFDAKIEGYRDMD
LTDILQMMGRAGRPAYDTTGTAIVYTKESKKMFYKHFLNVGFPVESSLHKVLDDHLGA
EITSGSITNKQEALDFLSWTFLFRRAHHNPTYYGIEDDTSTAGVSEHLSSLIDSTLEN
LRESQCVLLHGDDIVATPFLSISSYYYISHLTIRQLLKQIHDHATFQEVLRWLSLAVE
YNELPVRGGEIIMNEEMSQQSRYSVESTFTDEFELPMWDPHVKTFLLLQAHLSRVDLP
IADYIQDTVSVLDQSLRILQAYIDVASELGYFHTVLTMIKMMQCIKQGYWYEDDPVSV
LPGLQLRRIKDYTFSEQGFIEMTPQQKKKKLLTLEEIGRFGYKKLLNVFDQLTFGMTE
SEDTKKRFVSVCQRLPVLEGMKFEEQENNEVLTFYSKHLSSKHNNGFEVYCDKFPKIQ
KELWFLIGHKGDELLMIKRCQPKQMNKEVIIHCDLFIPEEIRGEELQFSLINDALGLR
YDMVHKLIS"
gene complement(<1037800..>1038501)
/gene="EFG1"
/locus_tag="YGR271C-A"
/gene_synonym="YGR272C"
/db_xref="GeneID:853188"
mRNA complement(<1037800..>1038501)
/gene="EFG1"
/locus_tag="YGR271C-A"
/gene_synonym="YGR272C"
/product="Efg1p"
/transcript_id="NM_001184474.3"
/db_xref="GeneID:853188"
CDS complement(1037800..1038501)
/gene="EFG1"
/locus_tag="YGR271C-A"
/gene_synonym="YGR272C"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0000321
re-entry into mitotic cell cycle after pheromone arrest
[PMID:16510898]"
/experiment="EXISTENCE:mutant phenotype:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12837249]"
/experiment="EXISTENCE:mutant phenotype:GO:0071027 nuclear
RNA surveillance [PMID:29481617]"
/note="Ribosome biogenesis factor required for maturation
of 18S rRNA; null mutant is sensitive to hydroxyurea and
is delayed in recovering from alpha-factor arrest; green
fluorescent protein (GFP)-fusion protein localizes to the
nucleolus"
/codon_start=1
/product="Efg1p"
/protein_id="NP_076891.4"
/db_xref="GeneID:853188"
/db_xref="SGD:S000007608"
/translation="MAKLQRKRSKALGSSLEMSQIMDAGTNKIKRRIRDLERLLKKKK
DILPSTVIIEKERNLQALRLELQNNELKNKIKANAKKYHMVRFFEKKKALRKYNRLLK
KIKESGADDKDLQQKLRATKIELCYVINFPKTEKYIALYPNDTPSTDPKGVELTNLRR
EQFLKLVAERMDANTLNVSFEEILKGKKLDEDSIGLTLSPDKDHEDGSQVSPTQDRKE
LDQVVGEDEKDDFFE"
gene complement(<1038715..>1039239)
/locus_tag="YGR273C"
/db_xref="GeneID:853190"
mRNA complement(<1038715..>1039239)
/locus_tag="YGR273C"
/product="uncharacterized protein"
/transcript_id="NM_001181402.1"
/db_xref="GeneID:853190"
CDS complement(1038715..1039239)
/locus_tag="YGR273C"
/note="hypothetical protein; expression downregulated by
treatment with 8-methoxypsoralen plus UVA irradiation; not
an essential gene; YGR273C has a paralog, YMR295C, that
arose from the whole genome duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011789.1"
/db_xref="GeneID:853190"
/db_xref="SGD:S000003505"
/translation="MQHFESSDKIEKDDDTSRIKLRSSSLAAPILEAVQEAQPFEEAT
FSNLQKIHPLTENSTCNGYVIYDKDGNLKSMKDTFGRNIKTPDISNPTRARNERPLDT
IRGFEYSITKDPRWLQELETSKLGFKPRPGFAVINQDSQASINLSQLEEKVMESQKKK
EKRHISRLSRLLCR"
gene complement(<1039895..>1043095)
/gene="TAF1"
/locus_tag="YGR274C"
/gene_synonym="KAT4; TAF130; TAF145"
/db_xref="GeneID:853191"
mRNA complement(<1039895..>1043095)
/gene="TAF1"
/locus_tag="YGR274C"
/gene_synonym="KAT4; TAF130; TAF145"
/product="histone acetyltransferase"
/transcript_id="NM_001181403.2"
/db_xref="GeneID:853191"
CDS complement(1039895..1043095)
/gene="TAF1"
/locus_tag="YGR274C"
/gene_synonym="KAT4; TAF130; TAF145"
/EC_number="2.3.1.48"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:10818000|PMID:12600943|PMID:10817999]"
/experiment="EXISTENCE:direct assay:GO:0004402 histone
acetyltransferase activity [PMID:8980232]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005669
transcription factor TFIID complex
[PMID:10788514|PMID:15448131]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0006366
transcription by RNA polymerase II
[PMID:12138208|PMID:15448131]"
/experiment="EXISTENCE:direct assay:GO:0017025 TBP-class
protein binding [PMID:11677244]"
/experiment="EXISTENCE:direct assay:GO:0060090 molecular
adaptor activity [PMID:15143185]"
/experiment="EXISTENCE:mutant phenotype:GO:0006366
transcription by RNA polymerase II
[PMID:9288742|PMID:9288741]"
/experiment="EXISTENCE:mutant phenotype:GO:0051123 RNA
polymerase II preinitiation complex assembly
[PMID:10713163|PMID:12600943|PMID:12840001]"
/experiment="EXISTENCE:mutant phenotype:GO:0060090
molecular adaptor activity [PMID:9844639|PMID:15143185]"
/note="TFIID subunit, involved in RNA pol II transcription
initiation; possesses in vitro histone acetyltransferase
activity but its role in vivo appears to be minor;
involved in promoter binding and G1/S progression;
relocalizes to the cytosol in response to hypoxia"
/codon_start=1
/product="histone acetyltransferase"
/protein_id="NP_011790.1"
/db_xref="GeneID:853191"
/db_xref="SGD:S000003506"
/translation="MVKQQGSGKTNLANEDEAYEAIFGGEFGSLEIGSYIGGDEGANS
KDYTEHLPDAVDFEDEDELADDDDDLPEESDANLHPAMMTMGAYDDVNENGAVLGIDS
NSLNMQLPEINGDLSQQFILEDDGGTPATSNALFMGMDANEIHLATETGVLDGSGANE
IGHSQLSIGGVNGNDMSINGGFIMEPDMSDGKHKKATKLDLINHEKYLLKKYFPDFEK
GKILKWNKLIYRRSVPYHWHSEISRVKKPFMPLNLKFKVQQDDKRLFNSRTISYVAPI
YQGKNNLLQSNSSASRRGLIHVSIDELFPIKEQQKKRKIIHDEKTISEDLLIATDDWD
QEKIINQGTSSTATLADSSMTPNLKFSGGYKLKSLIEDVAEDWQWDEDMIIDAKLKES
KHAELNMNDEKLLLMIEKTNNLAQQKQQLDSSNLILPLNETILQQKFNLSNDDKYQIL
KKTHQTKVRSTISNLNIQHSQPAINLQSPFYKVAVPRYQLRHFHRENFGSHIRPGTKI
VFSKLKARKRKRDKGKDVKESFSTSQDLTIGDTAPVYLMEYSEQTPVALSKFGMANKL
INYYRKANEQDTLRPKLPVGETHVLGVQDKSPFWNFGFVEPGHIVPTLYNNMIRAPVF
KHDISGTDFLLTKSSGFGISNRFYLRNINHLFTVGQTFPVEEIPGPNSRKVTSMKATR
LKMIIYRILNHNHSKAISIDPIAKHFPDQDYGQNRQKVKEFMKYQRDGPEKGLWRLKD
DEKLLDNEAVKSLITPEQISQVESMSQGLQFQEDNEAYNFDSKLKSLEENLLPWNITK
NFINSTQMRAMIQIHGVGDPTGCGEGFSFLKTSMKGGFVKSGSPSSNNNSSNKKGTNT
HSYNVAQQQKAYDEEIAKTWYTHTKSLSISNPFEEMTNPDEINQTNKHVKTDRDDKKI
LKIVRKKRDENGIIQRQTIFIRDPRVIQGYIKIKEQDKEDVNKLLEEDTSKINNLEEL
EKQKKLLQLELANLEKSQQRRAARQNSKRNGGATRTENSVDNGSDLAGVTDGKAARNK
GKNTTRRCATCGQIGHIRTNKSCPMYSSKDNPASPK"
gene <1043276..>1043749
/gene="RTT102"
/locus_tag="YGR275W"
/db_xref="GeneID:853192"
mRNA <1043276..>1043749
/gene="RTT102"
/locus_tag="YGR275W"
/product="Rtt102p"
/transcript_id="NM_001181404.3"
/db_xref="GeneID:853192"
CDS 1043276..1043749
/gene="RTT102"
/locus_tag="YGR275W"
/experiment="EXISTENCE:curator inference:GO:0006338
chromatin remodeling [PMID:14729968]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006337 nucleosome
disassembly [PMID:16492771]"
/experiment="EXISTENCE:direct assay:GO:0006338 chromatin
remodeling [PMID:17188033]"
/experiment="EXISTENCE:direct assay:GO:0006368
transcription elongation by RNA polymerase II
[PMID:17081996]"
/experiment="EXISTENCE:direct assay:GO:0015616 DNA
translocase activity [PMID:17188033]"
/experiment="EXISTENCE:direct assay:GO:0016514 SWI/SNF
complex [PMID:18644858|PMID:15506919]"
/experiment="EXISTENCE:direct assay:GO:0016586 RSC-type
complex [PMID:14729968]"
/experiment="EXISTENCE:mutant phenotype:GO:0007059
chromosome segregation [PMID:14729968]"
/note="Component of both the SWI/SNF and RSC chromatin
remodeling complexes; suggested role in chromosome
maintenance; possible weak regulator of Ty1 transposition;
protein abundance increases in response to DNA replication
stress"
/codon_start=1
/product="Rtt102p"
/protein_id="NP_011791.4"
/db_xref="GeneID:853192"
/db_xref="SGD:S000003507"
/translation="MDPQTLITKANKVSYYGNPTSKESWRYDWYQPSKVSSNVQQPQQ
QLGDMENNLEKYPFRYKTWLRNQEDEKNLQRESCEDILDLKEFDRRILKKSLMTSHTK
GDTSKATGAPSANQGDEALSVDDIRGAVGNSEAIPGLSAGVNNDNTKESKDVKMN"
gene complement(<1043819..>1045480)
/gene="RNH70"
/locus_tag="YGR276C"
/gene_synonym="REX1; RNA82"
/db_xref="GeneID:853193"
mRNA complement(<1043819..>1045480)
/gene="RNH70"
/locus_tag="YGR276C"
/gene_synonym="REX1; RNA82"
/product="Rnh70p"
/transcript_id="NM_001181405.1"
/db_xref="GeneID:853193"
CDS complement(1043819..1045480)
/gene="RNH70"
/locus_tag="YGR276C"
/gene_synonym="REX1; RNA82"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10359084]"
/experiment="EXISTENCE:genetic interaction:GO:0000467
exonucleolytic trimming to generate mature 3'-end of 5.8S
rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S
rRNA, LSU-rRNA) [PMID:10716935]"
/experiment="EXISTENCE:genetic interaction:GO:0034476 U5
snRNA 3'-end processing [PMID:10716935]"
/experiment="EXISTENCE:genetic interaction:GO:0042780 tRNA
3'-end processing [PMID:18456844]"
/experiment="EXISTENCE:mutant phenotype:GO:0000175
3'-5'-RNA exonuclease activity [PMID:10716935]"
/experiment="EXISTENCE:mutant phenotype:GO:0002107
generation of mature 3'-end of 5S rRNA generated by RNA
polymerase III [PMID:10716935]"
/experiment="EXISTENCE:mutant phenotype:GO:0042780 tRNA
3'-end processing [PMID:10716935|PMID:18456844]"
/note="3'-5' exoribonuclease; required for maturation of
3' ends of 5S rRNA and tRNA-Arg3 from dicistronic
transcripts"
/codon_start=1
/product="Rnh70p"
/protein_id="NP_011792.1"
/db_xref="GeneID:853193"
/db_xref="SGD:S000003508"
/translation="MQVEGPDTNFVSDLALGSKKRRLSKTSVQEDDHTNVVSEVNKNK
KKKKAKPMTCTLLKSVVEKGIGIKDVRDMTQYLLQAENNSPKWIDICNRSSLQKMIVL
FIPGLQPDDFENGKNTFNEISDDNFKYIPGEIASTFHTFPVMAPGSKMTLFSPYNSFI
NVGLSKMEKINKLKELQKKKKITINDLVLSEQQLVANDYPLDSGDTNFDTDWVQTVDF
THGGSHIFALDCEMCLSEQGLVLTRISLVNFDNEVIYEELVKPDVPIVDYLTRYSGIT
EEKLTVGAKKTLREVQKDLLKIISRSDILIGHSLQNDLKVMKLKHPLVVDTAIIYHHK
AGDPFKPSLKYLSETFLNKSIQNGEHDSVEDARACLELTKLKILNGLAFGIGINTENL
FTKLHRFEVKTVLLNDMIIKNHTEDDSKGQLIRCVEDDETWTHIHENLNKDVKLIVGR
IKNLERSRNYNKKPRKETPSFDASMVLHDIGQHLTQLYENATPGTMILIMSGTGDTRP
WNNLSTELEFIQDKKERLDKRREREPEIVEAIKLARGGVASFTVK"
gene complement(<1045644..>1046561)
/gene="CAB4"
/locus_tag="YGR277C"
/db_xref="GeneID:853194"
mRNA complement(<1045644..>1046561)
/gene="CAB4"
/locus_tag="YGR277C"
/product="putative pantetheine-phosphate
adenylyltransferase"
/transcript_id="NM_001181406.3"
/db_xref="GeneID:853194"
CDS complement(1045644..1046561)
/gene="CAB4"
/locus_tag="YGR277C"
/EC_number="2.7.7.3"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14690591]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14690591]"
/experiment="EXISTENCE:direct assay:GO:1990143
CoA-synthesizing protein complex [PMID:23789928]"
/experiment="EXISTENCE:genetic interaction:GO:0015937
coenzyme A biosynthetic process [PMID:19266201]"
/note="Subunit of the CoA-Synthesizing Protein Complex
(CoA-SPC); subunits of this complex are: Cab2p, Cab3p,
Cab4p, Cab5p, Sis2p and Vhs3p; involved in histone
acylation; probable pantetheine-phosphate
adenylyltransferase (PPAT); PPAT catalyzes the fourth step
in the biosynthesis of coenzyme A from pantothenate; null
mutant lethality is complemented by E. coli coaD (encoding
PPAT) and by human COASY"
/codon_start=1
/product="putative pantetheine-phosphate
adenylyltransferase"
/protein_id="NP_011793.3"
/db_xref="GeneID:853194"
/db_xref="SGD:S000003509"
/translation="MVEENSRVLIVLPYTPPSATLQRIIGQTIPFLRECQSQLDIVIV
PEFKTSFQLDSALGKMYSITRDVLLGYGMINSGINIIFNNIHFVESNLQWKVVLLPQE
STFETWKLELGQGQYHSIEHYALHDNIMEEIEGPKDANKFHVTALGGTFDHIHDGHKI
LLSVSTFITSQRLICGITCDELLQNKKYKELIEPYDTRCRHVHQFIKLLKPDLSVELV
PLRDVCGPTGKVPEIECLVVSRETVSGAETVNKTRIEKGMSPLAVHVVNVLGGREEDG
WSEKLSSTEIRRLLKSSASPTCTPQNPCV"
gene <1046731..>1048464
/gene="CWC22"
/locus_tag="YGR278W"
/db_xref="GeneID:853195"
mRNA <1046731..>1048464
/gene="CWC22"
/locus_tag="YGR278W"
/product="U2-type spliceosomal complex subunit CWC22"
/transcript_id="NM_001181407.3"
/db_xref="GeneID:853195"
CDS 1046731..1048464
/gene="CWC22"
/locus_tag="YGR278W"
/experiment="EXISTENCE:direct assay:GO:0000398 mRNA
splicing, via spliceosome [PMID:20956557]"
/experiment="EXISTENCE:direct assay:GO:0005684 U2-type
spliceosomal complex [PMID:11884590]"
/note="Spliceosome-associated protein that is required for
pre-mRNA splicing; necessary for Prp2p function at the
first catalytic step of splicing; has similarity to S.
pombe Cwf22p; CWC22 is an essential protein"
/codon_start=1
/product="U2-type spliceosomal complex subunit CWC22"
/protein_id="NP_011794.3"
/db_xref="GeneID:853195"
/db_xref="SGD:S000003510"
/translation="MSTATIQDEDIKFQRENWEMIRSHVSPIISNLTMDNLQESHRDL
FQVNILIGRNIICKNVVDFTLNKQNGRLIPALSALIALLNSDIPDIGETLAKELMLMF
VQQFNRKDYVSCGNILQCLSILFLYDVIHEIVILQILLLLLEKNSLRLVIAVMKICGW
KLALVSKKTHDMIWEKLRYILQTQELSSTLRESLETLFEIRQKDYKSGSQGLFILDPT
SYTVHTHSYIVSDEDEANKELGNFEKCENFNELTMAFDTLRQKLLINNTSDTNEGSNS
QLQIYDMTSTNDVEFKKKIYLVLKSSLSGDEAAHKLLKLKIANNLKKSVVDIIIKSSL
QESTFSKFYSILSERMITFHRSWQTAYNETFEQNYTQDIEDYETDQLRILGKFWGHLI
SYEFLPMDCLKIIKLTEEESCPQGRIFIKFLFQELVNELGLDELQLRLNSSKLDGMFP
LEGDAEHIRYSINFFTAIGLGLLTEDMRSRLTIIQEVEDAEEEEKKLREEEELEKLRK
KARESQPTQGPKIHESRLFLQKDTRENSRSRSPFTVETRKRARSRTPPRGSRNHRNRS
RTPPARRQRHR"
gene complement(<1048798..>1049958)
/gene="SCW4"
/locus_tag="YGR279C"
/db_xref="GeneID:853196"
mRNA complement(<1048798..>1049958)
/gene="SCW4"
/locus_tag="YGR279C"
/product="putative family 17 glucosidase"
/transcript_id="NM_001181408.1"
/db_xref="GeneID:853196"
CDS complement(1048798..1049958)
/gene="SCW4"
/locus_tag="YGR279C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005576
extracellular region [PMID:19129178]"
/experiment="EXISTENCE:direct assay:GO:0005773 vacuole
[PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:9748433|PMID:15781460]"
/experiment="EXISTENCE:genetic interaction:GO:0000747
conjugation with cellular fusion [PMID:9748433]"
/note="Cell wall protein with similarity to glucanases;
targeted to vacuole via AP-3 pathway; scw4 scw10 double
mutants exhibit defects in mating; SCW4 has a paralog,
SCW10, that arose from the whole genome duplication"
/codon_start=1
/product="putative family 17 glucosidase"
/protein_id="NP_011795.1"
/db_xref="GeneID:853196"
/db_xref="SGD:S000003511"
/translation="MRLSNLIASASLLSAATLAAPANHEHKDKRAVVTTTVQKQTTII
VNGAASTPVAALEENAVVNSAPAAATSTTSSAASVATAAASSSENNSQVSAAASPASS
SAATSTQSSSSSQASSSSSSGEDVSSFASGVRGITYTPYESSGACKSASEVASDLAQL
TDFPVIRLYGTDCNQVENVFKAKASNQKVFLGIYYVDQIQDGVNTIKSAVESYGSWDD
VTTVSIGNELVNGNQATPSQVGQYIDSGRSALKAAGYTGPVVSVDTFIAVINNPELCD
YSDYMAVNAHAYFDKNTVAQDSGKWLLEQIQRVWTACDGKKNVVITESGWPSKGETYG
VAVPSKENQKDAVSAITSSCGADTFLFTAFNDYWKADGAYGVEKYWGILSNE"
gene complement(<1050910..>1051725)
/gene="PXR1"
/locus_tag="YGR280C"
/gene_synonym="GNO1"
/db_xref="GeneID:853197"
mRNA complement(<1050910..>1051725)
/gene="PXR1"
/locus_tag="YGR280C"
/gene_synonym="GNO1"
/product="telomerase inhibitor"
/transcript_id="NM_001181409.1"
/db_xref="GeneID:853197"
CDS complement(1050910..1051725)
/gene="PXR1"
/locus_tag="YGR280C"
/gene_synonym="GNO1"
/experiment="EXISTENCE:direct assay:GO:0005654 nucleoplasm
[PMID:12107183]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:12107183]"
/experiment="EXISTENCE:genetic interaction:GO:0010521
telomerase inhibitor activity [PMID:14977919]"
/experiment="EXISTENCE:genetic interaction:GO:0032211
negative regulation of telomere maintenance via telomerase
[PMID:14977919]"
/experiment="EXISTENCE:mutant phenotype:GO:0000494 box C/D
sno(s)RNA 3'-end processing [PMID:12107183]"
/experiment="EXISTENCE:mutant phenotype:GO:0008047 enzyme
activator activity [PMID:24823796]"
/experiment="EXISTENCE:mutant phenotype:GO:0010521
telomerase inhibitor activity [PMID:14977919]"
/experiment="EXISTENCE:mutant phenotype:GO:0032211
negative regulation of telomere maintenance via telomerase
[PMID:14977919]"
/experiment="EXISTENCE:physical interaction:GO:0032211
negative regulation of telomere maintenance via telomerase
[PMID:14977919]"
/note="Essential protein involved in rRNA and snoRNA
maturation; competes with TLC1 RNA for binding to Est2p,
suggesting a role in negative regulation of telomerase;
human homolog inhibits telomerase; contains a G-patch RNA
interacting domain"
/codon_start=1
/product="telomerase inhibitor"
/protein_id="NP_011796.1"
/db_xref="GeneID:853197"
/db_xref="SGD:S000003512"
/translation="MGLAATRTKQRFGLDPRNTAWSNDTSRFGHQFLEKFGWKPGMGL
GLSPMNSNTSHIKVSIKDDNVGLGAKLKRKDKKDEFDNGECAGLDVFQRILGRLNGKE
SKISEELDTQRKQKIIDGKWGIHFVKGEVLASTWDPKTHKLRNYSNAKKRKREGDDSE
DEDDDDKEDKDSDKKKHKKHKKHKKDKKKDKKDKKEHKKHKKEEKRLKKEKRAEKTKE
TKKTSKLKSSESASNIPDAVNTRLSVRSKWIKQKRAALMDSKALNEIFMITND"
gene <1052824..>1057257
/gene="YOR1"
/locus_tag="YGR281W"
/gene_synonym="YRS1"
/db_xref="GeneID:853198"
mRNA <1052824..>1057257
/gene="YOR1"
/locus_tag="YGR281W"
/gene_synonym="YRS1"
/product="ATP-binding cassette transporter YOR1"
/transcript_id="NM_001181410.3"
/db_xref="GeneID:853198"
CDS 1052824..1057257
/gene="YOR1"
/locus_tag="YGR281W"
/gene_synonym="YRS1"
/EC_number="7.2.2.2"
/EC_number="7.6.2.3"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:9575223]"
/experiment="EXISTENCE:direct assay:GO:0008559 ABC-type
xenobiotic transporter activity [PMID:23270647]"
/experiment="EXISTENCE:direct assay:GO:0042908 xenobiotic
transport [PMID:23270647]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0008559
ABC-type xenobiotic transporter activity [PMID:23270647]"
/experiment="EXISTENCE:mutant phenotype:GO:0042908
xenobiotic transport [PMID:23270647]"
/note="Plasma membrane ATP-binding cassette (ABC)
transporter; multidrug transporter mediates export of many
different organic anions including oligomycin; homolog of
human cystic fibrosis transmembrane receptor (CFTR)"
/codon_start=1
/product="ATP-binding cassette transporter YOR1"
/protein_id="NP_011797.3"
/db_xref="GeneID:853198"
/db_xref="SGD:S000003513"
/translation="MTITVGDAVSETELENKSQNVVLSPKASASSDISTDVDKDTSSS
WDDKSLLPTGEYIVDRNKPQTYLNSDDIEKVTESDIFPQKRLFSFLHSKKIPEVPQTD
DERKIYPLFHTNIISNMFFWWVLPILRVGYKRTIQPNDLFKMDPRMSIETLYDDFEKN
MIYYFEKTRKKYRKRHPEATEEEVMENAKLPKHTVLRALLFTFKKQYFMSIVFAILAN
CTSGFNPMITKRLIEFVEEKAIFHSMHVNKGIGYAIGACLMMFVNGLTFNHFFHTSQL
TGVQAKSILTKAAMKKMFNASNYARHCFPNGKVTSFVTTDLARIEFALSFQPFLAGFP
AILAICIVLLIVNLGPIALVGIGIFFGGFFISLFAFKLILGFRIAANIFTDARVTMMR
EVLNNIKMIKYYTWEDAYEKNIQDIRTKEISKVRKMQLSRNFLIAMAMSLPSIASLVT
FLAMYKVNKGGRQPGNIFASLSLFQVLSLQMFFLPIAIGTGIDMIIGLGRLQSLLEAP
EDDPNQMIEMKPSPGFDPKLALKMTHCSFEWEDYELNDAIEEAKGEAKDEGKKNKKKR
KDTWGKPSASTNKAKRLDNMLKDRDGPEDLEKTSFRGFKDLNFDIKKGEFIMITGPIG
TGKSSLLNAMAGSMRKTDGKVEVNGDLLMCGYPWIQNASVRDNIIFGSPFNKEKYDEV
VRVCSLKADLDILPAGDMTEIGERGITLSGGQKARINLARSVYKKKDIYLFDDVLSAV
DSRVGKHIMDECLTGMLANKTRILATHQLSLIERASRVIVLGTDGQVDIGTVDELKAR
NQTLINLLQFSSQNSEKEDEEQEAVVAGELGQLKYESEVKELTELKKKATEMSQTANS
GKIVADGHTSSKEERAVNSISLKIYREYIKAAVGKWGFIALPLYAILVVGTTFCSLFS
SVWLSYWTENKFKNRPPSFYMGLYSFFVFAAFIFMNGQFTILCAMGIMASKWLNLRAV
KRILHTPMSYIDTTPLGRILNRFTKDTDSLDNELTESLRLMTSQFANIVGVCVMCIVY
LPWFAIAIPFLLVIFVLIADHYQSSGREIKRLEAVQRSFVYNNLNEVLGGMDTIKAYR
SQERFLAKSDFLINKMNEAGYLVVVLQRWVGIFLDMVAIAFALIITLLCVTRAFPISA
ASVGVLLTYVLQLPGLLNTILRAMTQTENDMNSAERLVTYATELPLEASYRKPEMTPP
ESWPSMGEIIFENVDFAYRPGLPIVLKNLNLNIKSGEKIGICGRTGAGKSTIMSALYR
LNELTAGKILIDNVDISQLGLFDLRRKLAIIPQDPVLFRGTIRKNLDPFNERTDDELW
DALVRGGAIAKDDLPEVKLQKPDENGTHGKMHKFHLDQAVEEEGSNFSLGERQLLALT
RALVRQSKILILDEATSSVDYETDGKIQTRIVEEFGDCTILCIAHRLKTIVNYDRILV
LEKGEVAEFDTPWTLFSQEDSIFRSMCSRSGIVENDFENRS"
gene complement(<1057783..>1058724)
/gene="BGL2"
/locus_tag="YGR282C"
/gene_synonym="SCW9"
/db_xref="GeneID:853199"
mRNA complement(<1057783..>1058724)
/gene="BGL2"
/locus_tag="YGR282C"
/gene_synonym="SCW9"
/product="glucan 1,3-beta-glucosidase"
/transcript_id="NM_001181411.1"
/db_xref="GeneID:853199"
CDS complement(1057783..1058724)
/gene="BGL2"
/locus_tag="YGR282C"
/gene_synonym="SCW9"
/EC_number="3.2.1.58"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:9748433|PMID:2509432]"
/experiment="EXISTENCE:direct assay:GO:0042973 glucan
endo-1,3-beta-D-glucosidase activity [PMID:8458852]"
/experiment="EXISTENCE:genetic interaction:GO:0031505
fungal-type cell wall organization [PMID:16986442]"
/experiment="EXISTENCE:mutant phenotype:GO:0042973 glucan
endo-1,3-beta-D-glucosidase activity [PMID:7851411]"
/note="Endo-beta-1,3-glucanase; major component of the
cell wall involved in cell wall maintenance; involved in
incorporation of newly synthesized mannoprotein molecules
into the cell wall; C-terminal region can form fibrillar
structures and has amyloidogenic potential"
/codon_start=1
/product="glucan 1,3-beta-glucosidase"
/protein_id="NP_011798.1"
/db_xref="GeneID:853199"
/db_xref="SGD:S000003514"
/translation="MRFSTTLATAATALFFTASQVSAIGELAFNLGVKNNDGTCKSTS
DYETELQALKSYTSTVKVYAASDCNTLQNLGPAAEAEGFTIFVGVWPTDDSHYAAEKA
ALQTYLPKIKESTVAGFLVGSEALYRNDLTASQLSDKINDVRSVVADISDSDGKSYSG
KQVGTVDSWNVLVAGYNSAVIEASDFVMANAFSYWQGQTMQNASYSFFDDIMQALQVI
QSTKGSTDITFWVGETGWPTDGTNFESSYPSVDNAKQFWKEGICSMRAWGVNVIVFEA
FDEDWKPNTSGTSDVEKHWGVFTSSDNLKYSLDCDFS"
gene complement(<1059015..>1060040)
/gene="UPA1"
/locus_tag="YGR283C"
/db_xref="GeneID:853200"
mRNA complement(<1059015..>1060040)
/gene="UPA1"
/locus_tag="YGR283C"
/product="putative methyltransferase"
/transcript_id="NM_001181412.3"
/db_xref="GeneID:853200"
CDS complement(1059015..1060040)
/gene="UPA1"
/locus_tag="YGR283C"
/experiment="EXISTENCE:curator inference:GO:0032259
methylation [PMID:21858014|PMID:22650761]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:14562095]"
/note="Putative SPOUT methyltransferase; may interact with
ribosomes, based on co-purification experiments; predicted
to be involved in ribosome biogenesis; contributes to the
methylation of Cse4-R37 in G2/M phase; overexpression
leads to chromosomal instability; null mutant is resistant
to fluconazole; GFP-fusion protein localizes to the
nucleolus; YGR283C has a paralog, YMR310C, that arose from
the whole genome duplication"
/codon_start=1
/product="putative methyltransferase"
/protein_id="NP_011799.3"
/db_xref="GeneID:853200"
/db_xref="SGD:S000003515"
/translation="MAVKHKSESLKHEEGAAKKAKTGLLKLKKIMDIESNVVKYSICI
PTTVIDNCNNLEQVTFTAYQIARTAVLFNVQEIIVLDQSKDKKHEKKSRSKETISDCL
LLATLLQYFVTPPNLLDTTFKKKNKLYLKCASTFPPLNQLPFMNASAEQHYKEGLSIA
RDSSKGKSDDALTNLVYIGKNQIITLSNQNIPNTARVTVDTERKEVVSPIDAYKGKPL
GYHVRMASTLNEVSEGYTKIVWVNSGDFHYDEELSKYHKVETKLPYIAKLKKSSTSEK
PCNILLIFGKWGHLKRCFRRSDLESSSLHHYFSGQLQFPASVPQGNIPIQDSLPIALT
MFQRWAS"
gene complement(<1060658..>1061590)
/gene="ERV29"
/locus_tag="YGR284C"
/db_xref="GeneID:853201"
mRNA complement(<1060658..>1061590)
/gene="ERV29"
/locus_tag="YGR284C"
/product="protein ERV29"
/transcript_id="NM_001181413.3"
/db_xref="GeneID:853201"
CDS complement(1060658..1061590)
/gene="ERV29"
/locus_tag="YGR284C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0030134
COPII-coated ER to Golgi transport vesicle
[PMID:11157978]"
/experiment="EXISTENCE:mutant phenotype:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:11157978]"
/experiment="EXISTENCE:mutant phenotype:GO:0097020 COPII
receptor activity [PMID:11711675]"
/note="Protein localized to COPII-coated vesicles;
involved in vesicle formation and incorporation of
specific secretory cargo; protein abundance increases in
response to DNA replication stress"
/codon_start=1
/product="protein ERV29"
/protein_id="NP_011800.3"
/db_xref="GeneID:853201"
/db_xref="SGD:S000003516"
/translation="MSYRGPIGNFGGMPMSSSQGPYSGGAQFRSNQNQSTSGILKQWK
HSFEKFASRIEGLTDNAVVYKLKPYIPSLSRFFIVATFYEDSFRILSQWSDQIFYLNK
WKHYPYFFVVVFLVVVTVSMLIGASLLVLRKQTNYATGVLCACVISQALVYGLFTGSS
FVLRNFSVIGGLLIAFSDSIVQNKTTFGMLPELNSKNDKAKGYLLFAGRILIVLMFIA
FTFSKSWFTVVLTIIGTICFAIGYKTKFASIMLGLILTFYNITLNNYWFYNNTKRDFL
KYEFYQNLSIIGGLLLVTNTGAGELSVDEKKKIY"
gene complement(<1061852..>1063153)
/gene="ZUO1"
/locus_tag="YGR285C"
/db_xref="GeneID:853202"
mRNA complement(<1061852..>1063153)
/gene="ZUO1"
/locus_tag="YGR285C"
/product="zuotin"
/transcript_id="NM_001181414.1"
/db_xref="GeneID:853202"
CDS complement(1061852..1063153)
/gene="ZUO1"
/locus_tag="YGR285C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276|PMID:20368619]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005840 ribosome
[PMID:17242366]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:25380751]"
/experiment="EXISTENCE:direct assay:GO:0051880
G-quadruplex DNA binding [PMID:32764578]"
/experiment="EXISTENCE:direct assay:GO:0071919
G-quadruplex DNA formation [PMID:41182900]"
/experiment="EXISTENCE:mutant phenotype:GO:0000054
ribosomal subunit export from nucleus [PMID:20368619]"
/experiment="EXISTENCE:mutant phenotype:GO:0005730
nucleolus [PMID:20368619]"
/experiment="EXISTENCE:mutant phenotype:GO:0006364 rRNA
processing [PMID:20368619]"
/experiment="EXISTENCE:mutant phenotype:GO:0006450
regulation of translational fidelity [PMID:15456889]"
/experiment="EXISTENCE:mutant phenotype:GO:0006452
translational frameshifting [PMID:16607023]"
/experiment="EXISTENCE:mutant phenotype:GO:0051083 'de
novo' cotranslational protein folding [PMID:11274393]"
/experiment="EXISTENCE:mutant phenotype:GO:0071919
G-quadruplex DNA formation [PMID:32764578]"
/note="Ribosome-associated co-chaperone and G-quadruplex
(G4) DNA binding protein; role in ribosome biogenesis;
nascent polypeptide chain chaperone with Ssz1p and
Ssb1/2p; ribosome-associated complex subunit that
stimulates Ssb1/2p ATPase activity to facilitate
interaction with nascent chains emerging from the exit
tunnel; promotes folding and stabilization of G4
structures; controls translation upon TORC1 inhibition;
contains a DnaJ domain; human gene DNAJC2 partially
complements the null mutant"
/codon_start=1
/product="zuotin"
/protein_id="NP_011801.1"
/db_xref="GeneID:853202"
/db_xref="SGD:S000003517"
/translation="MFSLPTLTSDITVEVNSSATKTPFVRRPVEPVGKFFLQHAQRTL
RNHTWSEFERIEAEKNVKTVDESNVDPDELLFDTELADEDLLTHDARDWKTADLYAAM
GLSKLRFRATESQIIKAHRKQVVKYHPDKQSAAGGSLDQDGFFKIIQKAFETLTDSNK
RAQYDSCDFVADVPPPKKGTDYDFYEAWGPVFEAEARFSKKTPIPSLGNKDSSKKEVE
QFYAFWHRFDSWRTFEFLDEDVPDDSSNRDHKRYIERKNKAARDKKKTADNARLVKLV
ERAVSEDPRIKMFKEEEKKEKERRKWEREAGARAEAEAKAKAEAEAKAKAESEAKANA
SAKADKKKAKEAAKAAKKKNKRAIRNSAKEADYFGDADKATTIDEQVGLIVDSLNDEE
LVSTADKIKANAAGAKEVLKESAKTIVDSGKLPSSLLSYFV"
gene complement(<1063813..>1064940)
/gene="BIO2"
/locus_tag="YGR286C"
/db_xref="GeneID:853203"
mRNA complement(<1063813..>1064940)
/gene="BIO2"
/locus_tag="YGR286C"
/product="biotin synthase"
/transcript_id="NM_001181415.1"
/db_xref="GeneID:853203"
CDS complement(1063813..1064940)
/gene="BIO2"
/locus_tag="YGR286C"
/EC_number="2.8.1.6"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095|PMID:24769239]"
/experiment="EXISTENCE:mutant phenotype:GO:0004076 biotin
synthase activity [PMID:10525840]"
/experiment="EXISTENCE:mutant phenotype:GO:0009102 biotin
biosynthetic process [PMID:10525840]"
/note="Biotin synthase; catalyzes the conversion of
dethiobiotin to biotin, which is the last step of the
biotin biosynthesis pathway; complements E. coli bioB
mutant"
/codon_start=1
/product="biotin synthase"
/protein_id="NP_011802.1"
/db_xref="GeneID:853203"
/db_xref="SGD:S000003518"
/translation="MMSTIYRHLSTARPALTKYATNAAVKSTTASSEASTLGALQYAL
SLDEPSHSWTKSQLKEIYHTPLLELTHAAQLQHRKWHDPTKVQLCTLMNIKSGGCSED
CKYCAQSSRNDTGLKAEKMVKVDEVIKEAEEAKRNGSTRFCLGAAWRDMKGRKSAMKR
IQEMVTKVNDMGLETCVTLGMVDQDQAKQLKDAGLTAYNHNIDTSREHYSKVITTRTY
DDRLQTIKNVQESGIKACTGGILGLGESEDDHIGFIYTLSNMSPHPESLPINRLVAIK
GTPMAEELADPKSKKLQFDEILRTIATARIVMPKAIIRLAAGRYTMKETEQFVCFMAG
CNSIFTGKKMLTTMCNGWDEDKAMLAKWGLQPMEAFKYDRS"
gene complement(<1067222..>1068991)
/gene="IMA1"
/locus_tag="YGR287C"
/db_xref="GeneID:853204"
mRNA complement(<1067222..>1068991)
/gene="IMA1"
/locus_tag="YGR287C"
/product="oligo-1,6-glucosidase IMA1"
/transcript_id="NM_001181416.3"
/db_xref="GeneID:853204"
CDS complement(1067222..1068991)
/gene="IMA1"
/locus_tag="YGR287C"
/EC_number="3.2.1.10"
/experiment="EXISTENCE:direct assay:GO:0004574
oligo-1,6-glucosidase activity
[PMID:15291818|PMID:20471265]"
/experiment="EXISTENCE:direct assay:GO:0004575 sucrose
alpha-glucosidase activity [PMID:24649402]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:genetic interaction:GO:0046352
disaccharide catabolic process [PMID:20471265]"
/experiment="EXISTENCE:mutant phenotype:GO:0046352
disaccharide catabolic process [PMID:20562106]"
/note="Major isomaltase
(alpha-1,6-glucosidase/alpha-methylglucosidase); required
for isomaltose utilization; preferred specificity for
isomaltose, alpha-methylglucoside, and palatinose, but
also exhibits alpha-1,2 glucosidase activity on sucrose
and kojibiose, and can cleave the 1,3-alpha linkage of
nigerose and turanose and the alpha-1,5 linkage of
leucrose in vitro; member of the IMA isomaltase family"
/codon_start=1
/product="oligo-1,6-glucosidase IMA1"
/protein_id="NP_011803.3"
/db_xref="GeneID:853204"
/db_xref="SGD:S000003519"
/translation="MTISSAHPETEPKWWKEATFYQIYPASFKDSNDDGWGDMKGIAS
KLEYIKELGADAIWISPFYDSPQDDMGYDIANYEKVWPTYGTNEDCFALIEKTHKLGM
KFITDLVINHCSSEHEWFKESRSSKTNPKRDWFFWRPPKGYDAEGKPIPPNNWKSYFG
GSAWTFDEKTQEFYLRLFCSTQPDLNWENEDCRKAIYESAVGYWLDHGVDGFRIDVGS
LYSKVVGLPDAPVVDKNSTWQSSDPYTLNGPRIHEFHQEMNQFIRNRVKDGREIMTVG
EMQHASDETKRLYTSASRHELSELFNFSHTDVGTSPLFRYNLVPFELKDWKIALAELF
RYINGTDCWSTIYLENHDQPRSITRFGDDSPKNRVISGKLLSVLLSALTGTLYVYQGQ
ELGQINFKNWPVEKYEDVEIRNNYNAIKEEHGENSEEMKKFLEAIALISRDHARTPMQ
WSREEPNAGFSGPSAKPWFYLNDSFREGINVEDEIKDPNSVLNFWKEALKFRKAHKDI
TVYGYDFEFIDLDNKKLFSFTKKYNNKTLFAALNFSSDATDFKIPNDDSSFKLEFGNY
PKKEVDASSRTLKPWEGRIYISE"
gene <1070293..>1071714
/gene="MAL13"
/locus_tag="YGR288W"
/db_xref="GeneID:853205"
mRNA <1070293..>1071714
/gene="MAL13"
/locus_tag="YGR288W"
/product="transcription factor MAL13"
/transcript_id="NM_001181417.1"
/db_xref="GeneID:853205"
CDS 1070293..1071714
/gene="MAL13"
/locus_tag="YGR288W"
/experiment="EXISTENCE:mutant phenotype:GO:0005975
carbohydrate metabolic process [PMID:3025617]"
/note="MAL-activator protein; part of complex locus MAL1;
nonfunctional in genomic reference strain S288C"
/codon_start=1
/product="transcription factor MAL13"
/protein_id="NP_011804.1"
/db_xref="GeneID:853205"
/db_xref="SGD:S000003520"
/translation="MTLTKQTCAKQACDCCRIRRVKCDGKRPCSSCLQNSLDCTYLQP
SRKRGPKSIRLRSLKRIAEVQRESGPNTIATAPVIYKRVPKKLIDQCLRLYHDNLYVI
WPLLSYDDLHKLLEEKYNDNYVYWFLTALSAATLSDLQTEIKSEEEVTFTGKQLSNLC
ISSCQQFDDLDNSNIFNIMTYYCLHRSFAQISNARTSYRLCCEAVGLITVAGLHREET
YGSLTFEEQQLRRKLYYLLLMTERYYAIYLHCATSLDATIAPPQLELVTDPQLSMDSF
LEMIRVFTVPGKCFFDALAADSTDASCTEESLKKIWNELHTTSSEIEPWSNGYIDISF
SRHWIRILAWKLAYQMRGSNFSLNANNGQIPIEIARDMLIDTYLTPENLYDVHGPGVP
VKTLEIATALVDIVGQYDHNMKLEAWNVLHDVCKFAFSLNHYNNDMLKRFSTKCQNAL
ITLPISKPLQLDGYPKDNEDIDP"
gene complement(<1073963..>1075813)
/gene="MAL11"
/locus_tag="YGR289C"
/gene_synonym="AGT1"
/db_xref="GeneID:853207"
mRNA complement(<1073963..>1075813)
/gene="MAL11"
/locus_tag="YGR289C"
/gene_synonym="AGT1"
/product="alpha-glucoside permease"
/transcript_id="NM_001181418.3"
/db_xref="GeneID:853207"
CDS complement(1073963..1075813)
/gene="MAL11"
/locus_tag="YGR289C"
/gene_synonym="AGT1"
/experiment="EXISTENCE:direct assay:GO:0005352
alpha-glucoside:proton symporter activity [PMID:12702465]"
/experiment="EXISTENCE:direct assay:GO:0015574 trehalose
transmembrane transporter activity [PMID:12702465]"
/experiment="EXISTENCE:direct assay:GO:0015771 trehalose
transport [PMID:12702465]"
/experiment="EXISTENCE:genetic interaction:GO:0015768
maltose transport [PMID:6371820]"
/experiment="EXISTENCE:mutant phenotype:GO:0005364
maltose:proton symporter activity
[PMID:8594329|PMID:1999393]"
/experiment="EXISTENCE:mutant phenotype:GO:0015768 maltose
transport [PMID:8594329]"
/experiment="EXISTENCE:mutant phenotype:GO:0046352
disaccharide catabolic process [PMID:20562106]"
/note="High-affinity maltose transporter (alpha-glucoside
transporter); inducible; encoded in the MAL1 complex
locus; broad substrate specificity that includes
maltotriose; required for isomaltose utilization"
/codon_start=1
/product="alpha-glucoside permease"
/protein_id="NP_011805.3"
/db_xref="GeneID:853207"
/db_xref="SGD:S000003521"
/translation="MKNIISLVSKKKAASKNEDKNISESSRDIVNQQEVFNTEDFEEG
KKDSAFELDHLEFTTNSAQLGDSDEDNENVINEMNATDDANEANSEEKSMTLKQALLK
YPKAALWSILVSTTLVMEGYDTALLSALYALPVFQRKFGTLNGEGSYEITSQWQIGLN
MCVLCGEMIGLQITTYMVEFMGNRYTMITALGLLTAYIFILYYCKSLAMIAVGQILSA
IPWGCFQSLAVTYASEVCPLALRYYMTSYSNICWLFGQIFASGIMKNSQENLGNSDLG
YKLPFALQWIWPAPLMIGIFFAPESPWWLVRKDRVAEARKSLSRILSGKGAEKDIQVD
LTLKQIELTIEKERLLASKSGSFFNCFKGVNGRRTRLACLTWVAQNSSGAVLLGYSTY
FFERAGMATDKAFTFSLIQYCLGLAGTLCSWVISGRVGRWTILTYGLAFQMVCLFIIG
GMGFGSGSSASNGAGGLLLALSFFYNAGIGAVVYCIVAEIPSAELRTKTIVLARICYN
LMAVINAILTPYMLNVSDWNWGAKTGLYWGGFTAVTLAWVIIDLPETTGRTFSEINEL
FNQGVPARKFASTVVDPFGKGKTQHDSLADESISQSSSIKQRELNAADKC"
gene <1076599..>1078353
/gene="MAL12"
/locus_tag="YGR292W"
/db_xref="GeneID:853209"
mRNA <1076599..>1078353
/gene="MAL12"
/locus_tag="YGR292W"
/product="alpha-glucosidase MAL12"
/transcript_id="NM_001181421.3"
/db_xref="GeneID:853209"
CDS 1076599..1078353
/gene="MAL12"
/locus_tag="YGR292W"
/EC_number="3.2.1.20"
/experiment="EXISTENCE:direct assay:GO:0004558
alpha-1,4-glucosidase activity [PMID:20471265]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836]"
/experiment="EXISTENCE:direct assay:GO:0033934 glucan
1,4-alpha-maltotriohydrolase activity [PMID:20471265]"
/experiment="EXISTENCE:genetic interaction:GO:0000025
maltose catabolic process [PMID:20471265]"
/experiment="EXISTENCE:genetic interaction:GO:0005987
sucrose catabolic process [PMID:20471265]"
/note="Maltase (alpha-D-glucosidase); inducible protein
involved in maltose catabolism; encoded in the MAL1
complex locus; hydrolyzes the disaccharides maltose,
turanose, maltotriose, and sucrose"
/codon_start=1
/product="alpha-glucosidase MAL12"
/protein_id="NP_011808.3"
/db_xref="GeneID:853209"
/db_xref="SGD:S000003524"
/translation="MTISDHPETEPKWWKEATIYQIYPASFKDSNNDGWGDLKGITSK
LQYIKDLGVDAIWVCPFYDSPQQDMGYDISNYEKVWPTYGTNEDCFELIDKTHKLGMK
FITDLVINHCSTEHEWFKESRSSKTNPKRDWFFWRPPKGYDAEGKPIPPNNWKSFFGG
SAWTFDETTNEFYLRLFASRQVDLNWENEDCRRAIFESAVGFWLDHGVDGFRIDTAGL
YSKRPGLPDSPIFDKTSKLQHPNWGSHNGPRIHEYHQELHRFMKNRVKDGREIMTVGE
VAHGSDNALYTSAARYEVSEVFSFTHVEVGTSPFFRYNIVPFTLKQWKEAIASNFLFI
NGTDSWATTYIENHDQARSITRFADDSPKYRKISGKLLTLLECSLTGTLYVYQGQEIG
QINFKEWPIEKYEDVDVKNNYEIIKKSFGKNSKEMKDFFKGIALLSRDHSRTPMPWTK
DKPNAGFTGPDVKPWFLLNESFEQGINVEQESRDDDSVLNFWKRALQARKKYKELMIY
GYDFQFIDLDSDQIFSFTKEYEDKTLFAALNFSGEEIEFSLPREGASLSFILGNYDDT
DVSSRVLKPWEGRIYLVK"
gene <1080306..>1080668
/gene="PAU12"
/locus_tag="YGR294W"
/db_xref="GeneID:853210"
mRNA <1080306..>1080668
/gene="PAU12"
/locus_tag="YGR294W"
/product="seripauperin PAU12"
/transcript_id="NM_001181423.1"
/db_xref="GeneID:853210"
CDS 1080306..1080668
/gene="PAU12"
/locus_tag="YGR294W"
/note="hypothetical protein; member of the seripauperin
multigene family encoded mainly in subtelomeric regions;
SWAT-GFP fusion protein localizes to both the endoplasmic
reticulum and vacuole"
/codon_start=1
/product="seripauperin PAU12"
/protein_id="NP_011810.1"
/db_xref="GeneID:853210"
/db_xref="SGD:S000003526"
/translation="MVKLTSIAAGVAAIAATASATTTLAQSDERVNLVELGVYVSDIR
AHLAQYYSFQAAHPTETYPVEIAEAVFNYGDFTTMLTGIAPDQVTRMITGVPWYSSRL
KPAISSALSKDGIYTIAN"
gene complement(<1081584..>1082729)
/gene="COS6"
/locus_tag="YGR295C"
/db_xref="GeneID:853212"
mRNA complement(<1081584..>1082729)
/gene="COS6"
/locus_tag="YGR295C"
/product="Cos6p"
/transcript_id="NM_001181424.1"
/db_xref="GeneID:853212"
CDS complement(1081584..1082729)
/gene="COS6"
/locus_tag="YGR295C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:25942624]"
/note="Endosomal protein involved in turnover of plasma
membrane proteins; member of the DUP380 subfamily of
conserved, often subtelomeric COS genes; required for the
multivesicular vesicle body sorting pathway that
internalizes plasma membrane proteins for degradation; Cos
proteins provide ubiquitin in trans for nonubiquitinated
cargo proteins"
/codon_start=1
/product="Cos6p"
/protein_id="NP_011811.1"
/db_xref="GeneID:853212"
/db_xref="SGD:S000003527"
/translation="MKENELKNEKSVDVLSVKQLESQKTVLPQDLFRSSFTWFCYEIY
KSLVFRIWMLLWLPLSVWWKLSNNWIYPLMVSLLVLFWGPVFVLVIFRLSRKRSLSKQ
LTQFCKEITKNTPSSDPHDWEVVAANLNSYFYENKAWNTKYFFFSAMSCQEAFRTTLL
EPFSLKKDEAAKVKSFKDSVPYIEEALEVYFTEVEKQWKLFNSEKSWSPVGLEDAKLP
KEAYRFKLTWVLKRIFNRRCLPLFLFYLHNVFISRNDGTIARPLFLVVLFFIMTRDFR
NMRMIVLSVKMEHKMQFLSTIINEQESGANGWDEIAKKMNRYLFEKKVWKNEEFFFDG
IDCEWFFSHFFYRVLSAKKSMRALSLNVELWPYIKEAQLSCSEESLA"
telomere 1083635..1090940
/note="TEL07R; Telomeric region on the right arm of
Chromosome VII; annotated components include an X element
core sequence, X element combinatorial repeats, and a long
Y' element; TEL07R does have telomeric repeats
(TEL07R-TR), but they are missing from the genome
annotation due to difficulties encountered during
sequencing and/or assembly"
/db_xref="SGD:S000028960"
rep_origin 1083795..1084863
/note="ARS736; Putative replication origin; identified in
multiple array studies, not yet confirmed by plasmid-based
assay"
/db_xref="SGD:S000130170"
gene <1084864..>1090591
/gene="YRF1-3"
/locus_tag="YGR296W"
/gene_synonym="YRF1"
/db_xref="GeneID:853213"
mRNA join(<1084864..1084882,1085031..>1090591)
/gene="YRF1-3"
/locus_tag="YGR296W"
/gene_synonym="YRF1"
/product="Y' element ATP-dependent helicase protein 1 copy
3"
/transcript_id="NM_001181425.3"
/db_xref="GeneID:853213"
CDS join(1084864..1084882,1085031..1090591)
/gene="YRF1-3"
/locus_tag="YGR296W"
/gene_synonym="YRF1"
/experiment="EXISTENCE:curator inference:GO:0005634
nucleus [PMID:9837911]"
/experiment="EXISTENCE:direct assay:GO:0003678 DNA
helicase activity [PMID:9837911]"
/experiment="EXISTENCE:genetic interaction:GO:0000722
telomere maintenance via recombination [PMID:9837911]"
/note="Helicase encoded by the Y' element of subtelomeric
regions; highly expressed in the mutants lacking the
telomerase component TLC1; potentially phosphorylated by
Cdc28p"
/codon_start=1
/product="Y' element ATP-dependent helicase protein 1 copy
3"
/protein_id="NP_011812.3"
/db_xref="GeneID:853213"
/db_xref="SGD:S000003528"
/translation="MEIENEQICTCIAQILHLLNSLIITFLDDDKTETGQSFVYIDGF
LVKKHNNQHTIVNFETYKNKMKVSDRRKFEKANFDEFESALNNKNDLVHCPSITLFES
IPTEVRSFYEDEKSGLIKVVKFRTGAMDRKRSFEKIVVSVMVGKNVQKFLTFVEDEPD
FQGGPIPSKYLIPKKINLMVYTLFQVHTLKFNRKDYDTLSLFYLNRGYYNELSFRVLE
RCYEIASARPNDSSTMRTFTDFVSGTPIVRGLQKSTIRKYGYNLAPYMFLLLHVDELS
IFSAYQASLPGEKKVDTERLKRDLCPRKPTEIKYFSQICNDMMNKKDRLGDILHIILR
ACALNFGAGPRGGAGDEEDRSITNEEPIIPSVDEHGLKVCKLRSPNTPRRLRKTLDAV
KALLVSSCACTARDLDIFDDNNGVAMWKWIKILYHEVAQETALKDSYRITLVPSSDGV
SVCGKLFNREYVRGFYFACKAQFDNLWEELNDCFYMPTVVDIASLILRNREVLFREPK
RGIDEYLENDSFLQMIPVKYREIVLPKLRRDTNKMTAALKNKVTVAIDELTVPLMWMI
HFAVGYPYRYPELQLLAFAGPQRNVYVDDTTRRIQLYTDYNKNGSSEPRLKTLDGLTS
DYVFYFVTVLRQMQICALGNSYDAFNHDPWMDVVGFEDPDQVTNRDISRIVLYSYMFL
NTAKGCLVEYATFRQYMRELPKNAPQKLNFREMRQGLIALGRHCVGSRFETDLYESAT
SELMANHSVQTGRNIYGVDSFSLTSVSGTTATLLQERASERWIQWLGLESDYHCSFSS
TRNAEDVVAGEAASSDHHQKISRVTRKRPREPKSTNDILVAGQKLFGSSFEFRDLHQL
RLCHEIYMADTPSVAVQAPPGYGKTELFHLPLIALASKGDVKYVSFLFVPYTVLLANC
MIRLSRCGCLNVAPVRNFIEEGCDGVTDLYVGIYDDLASTNFTDRIAAWENIVECTFR
TNNVKLGYLIVDEFHNFETEVYRQSQFGGITNLDFDAFEKAIFLSGTAPEAVADAALQ
RIGLTGLAKKSMDINELKRSEDLSRGLSSYPTRMFNLIKEKSEVPLGHVHKIWKKVES
QPEEALKLLLALFEIEPESKAIVVASTTNEVEELACSWRKYFRVVWIHGKLGAAEKVS
RTKEFVTDGSMRVLIGTKLVTEGIDIKQLMMVIMLDNRLNIIELIQGVGRLRDGGLCY
LLSRKNSWAARNRKGELPPIKEGCITEQVREFYGLESKKGKKGQHVGCCGSRTDLSAD
TVELIERMDRLAEKQATASMSIVALPSSFQESNSSDRCRKYCSSDEDSDTCIHGSANA
STNATTNSSTNATTTASTNVRTSATTTASINVRTSATTTESTNSSTNATTTASTNVRT
SATTTASINVRTSATTTESTNSNTSATTTESTDSNTSATTTESTDSNTSATTTASTNS
STNATTTASTNSSTNATTTESTNASAKEDANKDGNAEDNRFHPVTDINKESYKRKGSQ
MVLLERKKLKAQFPNTSENMNVLQFLGFRSDEIKHLFLYGIDVYFCPEGVFTQYGLCK
GCQKMFELCVCWAGQKVSYRRMAWEALAVERMLRNDEEYKEYLEDIEPYHGDPVGYLK
YFSVKRGEIYSQIQRNYAWYLAITRRRETISVLDSTRGKQGSQVFRMSGRQIKELYYK
VWSNLRESKTEVLQYFLNWDEKKCREEWEAKDDTVFVEALEKVGVFQRLRSMTSAGLQ
GPQYVKLQFSRHHRQLRSRYELSLGMHLRDQLALGVTPSKVPHWTAFLSMLIGLFCNK
TFRQKLEYLLEQISEVWLLPHWLDLANVEVLAADNTRVPLYMLMVAVHKELDSDDVPD
GRFDILLCRDSSREVGE"
CONTIG join(BK006941.2:1..1090940)
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