LOCUS NC_001147 1091291 bp DNA linear CON 23-JAN-2026
DEFINITION Saccharomyces cerevisiae S288C chromosome XV, complete sequence.
ACCESSION NC_001147
VERSION NC_001147.6
DBLINK BioProject: PRJNA128
Assembly: GCF_000146045.2
KEYWORDS RefSeq.
SOURCE Saccharomyces cerevisiae S288C
ORGANISM Saccharomyces cerevisiae S288C
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
Saccharomyces.
REFERENCE 1 (bases 1 to 1091291)
AUTHORS Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M.,
Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S.,
Weng,S. and Cherry,J.M.
TITLE New data and collaborations at the Saccharomyces Genome Database:
updated reference genome, alleles, and the Alliance of Genome
Resources
JOURNAL Genetics 220 (4) (2022)
PUBMED 34897464
REFERENCE 2 (bases 1 to 1091291)
AUTHORS Dujon,B., Albermann,K., Aldea,M., Alexandraki,D., Ansorge,W.,
Arino,J., Benes,V., Bohn,C., Bolotin-Fukuhara,M., Bordonne,R.,
Boyer,J., Camasses,A., Casamayor,A., Casas,C., Cheret,G.,
Cziepluch,C., Daignan-Fornier,B., Dang,D.V., de Haan,M., Delius,H.,
Durand,P., Fairhead,C., Feldmann,H., Gaillon,L., Kleine,K. et al.
TITLE The nucleotide sequence of Saccharomyces cerevisiae chromosome XV
JOURNAL Nature 387 (6632 SUPPL), 98-102 (1997)
PUBMED 9169874
REFERENCE 3 (bases 1 to 1091291)
AUTHORS Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
Oliver,S.G.
TITLE Life with 6000 genes
JOURNAL Science 274 (5287), 546 (1996)
PUBMED 8849441
REFERENCE 4 (bases 1 to 1091291)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (23-JAN-2026) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 5 (bases 1 to 1091291)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (16-JAN-2015) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Protein update by submitter
REFERENCE 6 (bases 1 to 1091291)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (04-MAY-2012) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Protein update by submitter
REFERENCE 7 (bases 1 to 1091291)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (31-MAR-2011) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Sequence update by submitter
REFERENCE 8 (bases 1 to 1091291)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (27-MAY-2010) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Protein update by submitter
REFERENCE 9 (bases 1 to 1091291)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (14-DEC-2009) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
COMMENT REVIEWED REFSEQ: This record has been curated by SGD. The reference
sequence is identical to BK006948.
On Apr 26, 2011 this sequence version replaced NC_001147.5.
##Genome-Annotation-Data-START##
Annotation Provider :: SGD
Annotation Status :: Full Annotation
Annotation Version :: R64-4-1
URL :: http://www.yeastgenome.org/
##Genome-Annotation-Data-END##
FEATURES Location/Qualifiers
source 1..1091291
/organism="Saccharomyces cerevisiae S288C"
/mol_type="genomic DNA"
/strain="S288C"
/db_xref="taxon:559292"
/chromosome="XV"
telomere complement(1..847)
/note="TEL15L; Telomeric region on the left arm of
Chromosome XV; composed of an X element core sequence, X
element combinatorial repeats, and a terminal stretch of
telomeric repeats"
/db_xref="SGD:S000028929"
gene <585..>740
/locus_tag="YOL166W-A"
/db_xref="GeneID:1466472"
mRNA <585..>740
/locus_tag="YOL166W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184623.1"
/db_xref="GeneID:1466472"
CDS 585..740
/locus_tag="YOL166W-A"
/note="hypothetical protein; identified by gene-trapping,
microarray-based expression analysis, and genome-wide
homology searching"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878160.1"
/db_xref="GeneID:1466472"
/db_xref="SGD:S000028709"
/translation="MHGACLSGLYPLPFTHKFHDYLHFNIYISFGGPKYCITALNTYV
ILFYTVY"
gene complement(<1647..>2078)
/gene="AAD15"
/locus_tag="YOL165C"
/db_xref="GeneID:853999"
mRNA complement(<1647..>2078)
/gene="AAD15"
/locus_tag="YOL165C"
/product="putative aryl-alcohol dehydrogenase"
/transcript_id="NM_001183418.1"
/db_xref="GeneID:853999"
CDS complement(1647..2078)
/gene="AAD15"
/locus_tag="YOL165C"
/note="Putative aryl-alcohol dehydrogenase; similar to P.
chrysosporium aryl-alcohol dehydrogenase; mutational
analysis has not yet revealed a physiological role; AAD15
has a paralog, AAD3, that arose from a segmental
duplication; members of the AAD gene family comprise three
pairs (AAD3 + AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14)
whose two genes are more related to one another than to
other members of the family"
/codon_start=1
/product="putative aryl-alcohol dehydrogenase"
/protein_id="NP_014477.1"
/db_xref="GeneID:853999"
/db_xref="SGD:S000005525"
/translation="MARHFGMALAPWDVMGGGRFQSKKAMEERRKNGECIRSFVGASE
QTDAEIKISEALAKVAEEHGTESVTAIAIAYVRSKAKNVFPSVEGGKIEDLKENIKAL
SIDLTPDNIKYLENVVPFDIGFPNTFIVLNSLTQKYGTNNV"
repeat_region complement(2894..3223)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007148"
repeat_region 3809..3986
/note="Ty4 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007160"
gene <4130..>4312
/locus_tag="YOL164W-A"
/db_xref="GeneID:1466473"
mRNA <4130..>4312
/locus_tag="YOL164W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184575.1"
/db_xref="GeneID:1466473"
CDS 4130..4312
/locus_tag="YOL164W-A"
/note="hypothetical protein; identified by fungal homology
and RT-PCR"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878161.1"
/db_xref="GeneID:1466473"
/db_xref="SGD:S000028580"
/translation="MFAKLYRGLTYVTNKILSTLLSHHSIHYCINSFKPTTLAIIILL
HPCCRCNVYIIRCIHH"
gene <6175..>8115
/gene="BDS1"
/locus_tag="YOL164W"
/db_xref="GeneID:854000"
mRNA <6175..>8115
/gene="BDS1"
/locus_tag="YOL164W"
/product="sulfuric ester hydrolase"
/transcript_id="NM_001183417.1"
/db_xref="GeneID:854000"
CDS 6175..8115
/gene="BDS1"
/locus_tag="YOL164W"
/experiment="EXISTENCE:mutant phenotype:GO:0018741 linear
primary-alkylsulfatase activity [PMID:15947202]"
/experiment="EXISTENCE:mutant phenotype:GO:0018909 dodecyl
sulfate metabolic process [PMID:15947202]"
/note="Bacterially-derived sulfatase; required for use of
alkyl sulfates as sulfur source"
/codon_start=1
/product="sulfuric ester hydrolase"
/protein_id="NP_014478.1"
/db_xref="GeneID:854000"
/db_xref="SGD:S000005524"
/translation="MIGAFKRNRGSSQSFAKECQPSTLKANLEVAKELPFSDRRDFED
ATQGYIGSLSDEQIIGPDGGVVWCMKSYGFLEPETPANTVNPSLWRQAQLNAIHGLFK
ITDNVYQVRGLDISNMTIIEGNTSLIIIDTLFTTETAQESLKLYYRHRPQKPVRTVIY
THSHSDHYGGVKGIVKEADVKSGEVQIIAPVGFMESVVAENILAGNAMHRRSQYQFGM
LLSPSVKGHVDCGIGKAASHGTVTLIAPTIIIEEPVEERTIDGVDFVFQLAPGSEAPS
EMLIYMPQQRVLNMAEDVTHHMHNLYALRGVEVRDGNQWAKYIDAARVAFGSKTDVLI
AQHHWPTTGQMRINELLKKQRDMYKFIHDQTLRLLNQGYTSRDIAETLRMPSSLEQEW
STRGYYGTLSHNVKAVYQKYLGWYDANPANLNPLPPVAYAKKAVEYMGGADAVLARAY
KDFQKGEFRWVASVVNQLVFADPNNHQARELCADALEQLGYQAEASTWRNAYLVGAME
LRQGVPKRRSTGKRNNIAVLNNEMFFDFLAVRLNATKAEGKIIVSNWCFINSNERFVI
TLENCALTYIQGWQTDADATITLKRTTFEALLANEITMVDFLRSKEVEIEGNRLRIEE
LLKLFDDFDQSFPVVEPMGGST"
gene <9597..>10106
/locus_tag="YOL163W"
/db_xref="GeneID:854001"
mRNA <9597..>10106
/locus_tag="YOL163W"
/product="uncharacterized protein"
/transcript_id="NM_001183416.1"
/db_xref="GeneID:854001"
CDS 9597..10106
/locus_tag="YOL163W"
/note="hypothetical protein; proposed to be an inactive
evolutionary remnant that along with neighboring gene
YOL162W may have constituted a second sulfonate
transporter and SOA1 paralog that is intact in S. uvarum,
S. eubayanus and S. arboricola and named SOA2; member of
the Dal5p subfamily of the major facilitator family"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014479.1"
/db_xref="GeneID:854001"
/db_xref="SGD:S000005523"
/translation="MIAWSLVATLQCKMTGKSSFYTCRALMGLFEGGFVADLVLWMSY
FYSSSELSIRLSFFWVTLSLTQIITSIVAFGVFHMRGIGGMAGWQWLFLIERIFTLVI
GISAYFLMVPSVVQTKKPWSKKGWFTEREEKIIVNKILRDDPTKGDMNNRQGMSLKML
WQGITDYYI"
gene <10119..>10766
/locus_tag="YOL162W"
/db_xref="GeneID:854002"
mRNA <10119..>10766
/locus_tag="YOL162W"
/product="uncharacterized protein"
/transcript_id="NM_001183415.1"
/db_xref="GeneID:854002"
CDS 10119..10766
/locus_tag="YOL162W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/note="hypothetical protein; proposed to be an inactive
evolutionary remnant that along with neighboring gene
YOL163W may have constituted a second sulfonate
transporter and SOA1 paralog that is intact in S. uvarum,
S. eubayanus and S. arboricola and named SOA2; member of
the Dal5p subfamily of the major facilitator family"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014480.1"
/db_xref="GeneID:854002"
/db_xref="SGD:S000005522"
/translation="MGLLAYIPTNVLATYLTLVLRSIGFTTFQANLLAIPNFVLHILL
LFGLTWSTEKCNNRLGLSLLQPLYTVPLLAVLRFWKGTMFNKWGTYAIITLILDNPYI
HAICVSLCSRNSQSVKTRTVSTCLYNMFVQAGLIISSNIYAKSDAPLYRKGNGVLFGL
ALFMFPILIGSKLIYVYINKQRDKRWNAMSEEEKDHYLSTTSDAGSRRLDFRFYH"
gene complement(<11549..>11911)
/gene="PAU20"
/locus_tag="YOL161C"
/db_xref="GeneID:854003"
mRNA complement(<11549..>11911)
/gene="PAU20"
/locus_tag="YOL161C"
/product="seripauperin PAU20"
/transcript_id="NM_001183414.1"
/db_xref="GeneID:854003"
CDS complement(11549..11911)
/gene="PAU20"
/locus_tag="YOL161C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/note="hypothetical protein; member of the seripauperin
multigene family encoded mainly in subtelomeric regions;
SWAT-GFP and mCherry fusion proteins localize to the
vacuole; expression induced by low temperature and also by
anaerobic conditions; induced during alcoholic
fermentation"
/codon_start=1
/product="seripauperin PAU20"
/protein_id="NP_014481.1"
/db_xref="GeneID:854003"
/db_xref="SGD:S000005521"
/translation="MVKLTSIAAGVAAIAAGASATTTLAQSDERVNLVELGVYVSDIR
AHLAQYYMFQAAHPTETYPVEVAEAVFNYGDFTTMLTGISPDQVTRMITGVPWYSTRL
KPAISKALSKDGIYTIAN"
gene <14313..>14654
/locus_tag="YOL160W"
/db_xref="GeneID:854004"
mRNA <14313..>14654
/locus_tag="YOL160W"
/product="uncharacterized protein"
/transcript_id="NM_001348873.1"
/db_xref="GeneID:854004"
CDS 14313..14654
/locus_tag="YOL160W"
/note="hypothetical protein; conserved across S.
cerevisiae strains"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335811.1"
/db_xref="GeneID:854004"
/db_xref="SGD:S000005520"
/translation="MENIAFICLQSCTRGIYGCQFYSATLENYHNISFPIFLLQTTLF
NHCISLNWSKAVFNRIKRRKYMMELKKHCYNYQVSRIGNRKKNGCFFLKMANNRVFSV
KNSPRLLLFFI"
gene complement(<15233..>15505)
/locus_tag="YOL159C-A"
/db_xref="GeneID:854005"
mRNA complement(<15233..>15505)
/locus_tag="YOL159C-A"
/product="uncharacterized protein"
/transcript_id="NM_001184493.1"
/db_xref="GeneID:854005"
CDS complement(15233..15505)
/locus_tag="YOL159C-A"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:21777356]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:21777356|PMID:14562095]"
/note="hypothetical protein; overexpression affects
endocytic protein trafficking; identified by sequence
comparison with hemiascomycetous yeast species"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_076909.1"
/db_xref="GeneID:854005"
/db_xref="SGD:S000007627"
/translation="MQYCELDLSGQWLDTVYCEENFSDFVFIKFLNPSQFEEKIYCYT
LHITKRTLENKRLLLYYEDEFKKHGHDINELVGDGIILRSCWNPRQ"
gene complement(<17281..>17796)
/gene="CSS3"
/locus_tag="YOL159C"
/db_xref="GeneID:854006"
mRNA complement(<17281..>17796)
/gene="CSS3"
/locus_tag="YOL159C"
/product="Css3p"
/transcript_id="NM_001183412.1"
/db_xref="GeneID:854006"
CDS complement(17281..17796)
/gene="CSS3"
/locus_tag="YOL159C"
/experiment="EXISTENCE:direct assay:GO:0005576
extracellular region [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="hypothetical protein, secreted when constitutively
expressed; SWAT-GFP, seamless-GFP and mCherry fusion
proteins localize to the cell periphery, SWAT-GFP fusion
also localizes to the extracellular region, and mCherry
fusion also localizes to the vacuole; deletion mutants are
viable and have elevated levels of Ty1 retrotransposition
and Ty1 cDNA"
/codon_start=1
/product="Css3p"
/protein_id="NP_014483.1"
/db_xref="GeneID:854006"
/db_xref="SGD:S000005519"
/translation="MVPLFGLFCIFSQLYSLCSAYVDITSGYQVFFNLPTNMTNNQIC
WLFQASYYDIYSDKSGRTLRTGRFEPGDQQSLIYRDTLVELEAITDSYEYSNLDLSTY
NGPEPYNSETDYCTDIMDLVMRVYDEEGHYVHPVANNSTNACAHPTPPTLNNLLISNY
SDGRNYKESSI"
repeat_region complement(18302..18561)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007149"
gene complement(<19491..>21311)
/gene="ENB1"
/locus_tag="YOL158C"
/gene_synonym="ARN4"
/db_xref="GeneID:854007"
mRNA complement(<19491..>21311)
/gene="ENB1"
/locus_tag="YOL158C"
/gene_synonym="ARN4"
/product="Enb1p"
/transcript_id="NM_001183411.1"
/db_xref="GeneID:854007"
CDS complement(19491..21311)
/gene="ENB1"
/locus_tag="YOL158C"
/gene_synonym="ARN4"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:17714436]"
/experiment="EXISTENCE:direct assay:GO:0033101 cellular
bud membrane [PMID:17714436]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0015620
ferric-enterobactin transmembrane transporter activity
[PMID:10831226]"
/experiment="EXISTENCE:mutant phenotype:GO:0015685
ferric-enterobactin import into cell [PMID:10831226]"
/note="Ferric enterobactin transmembrane transporter;
expressed under conditions of iron deprivation; member of
the major facilitator superfamily; expression is regulated
by Rcs1p and affected by chloroquine treatment"
/codon_start=1
/product="Enb1p"
/protein_id="NP_014484.1"
/db_xref="GeneID:854007"
/db_xref="SGD:S000005518"
/translation="MLETDHSRNDNLDDKSTVCYSEKTDSNVEKSTTSGLRRIDAVNK
VLSDYSSFTAFGVTFSSLKTALLVALFLQGYCTGLGGQISQSIQTYAANSFGKHSQVG
SINTVKSIVASVVAVPYARISDRFGRIECWIFALVLYTIGEIISAATPTFSGLFAGIV
IQQFGYSGFRLLATALTGDLSGLRDRTFAMNIFLIPVIINTWVSGNIVSSVAGNVAPY
KWRWGYGIFCIIVPISTLILVLPYVYAQYISWRSGKLPPLKLKEKGQTLRQTLWKFAD
DINLIGVILFTAFLVLVLLPLTIAGGATSKWREGHIIAMIVVGGCLGFIFLIWELKFA
KNPFIPRVYLGDPTIYVALLMEFVWRLGLQIELEYLVTVLMVAFGESTLSAQRIAQLY
NFLQSCTNIVVGIMLHFYPHPKVFVVAGSLLGVIGMGLLYKYRVVYDGISGLIGAEIV
VGIAGGMIRFPMWTLVHASTTHNEMATVTGLLMSVYQIGDAVGASIAGAIWTQRLAKE
LIQRLGSSLGMAIYKSPLNYLKKYPIGSEVRVQMIESYSKIQRLLIIVSISFAAFNAV
LCFFLRGFTVNKKQSLSAEEREKEKLKIKQQSWLRRVIGY"
gene complement(<22525..>24294)
/gene="IMA2"
/locus_tag="YOL157C"
/db_xref="GeneID:854008"
mRNA complement(<22525..>24294)
/gene="IMA2"
/locus_tag="YOL157C"
/product="oligo-1,6-glucosidase IMA2"
/transcript_id="NM_001183410.1"
/db_xref="GeneID:854008"
CDS complement(22525..24294)
/gene="IMA2"
/locus_tag="YOL157C"
/EC_number="3.2.1.10"
/experiment="EXISTENCE:direct assay:GO:0004574
oligo-1,6-glucosidase activity [PMID:20471265]"
/experiment="EXISTENCE:direct assay:GO:0004575 sucrose
alpha-glucosidase activity [PMID:24649402]"
/experiment="EXISTENCE:genetic interaction:GO:0046352
disaccharide catabolic process
[PMID:20562106|PMID:20471265]"
/experiment="EXISTENCE:mutant phenotype:GO:0004574
oligo-1,6-glucosidase activity [PMID:20562106]"
/note="Isomaltase
(alpha-1,6-glucosidase/alpha-methylglucosidase); preferred
specificity for isomaltose, alpha-methylglucoside, and
palatinose, but also exhibits alpha-1,2 glucosidase
activity on sucrose and kojibiose, and can cleave the
1,3-alpha linkage of nigerose and turanose and the
alpha-1,5 linkage of leucrose in vitro; not required for
isomaltose utilization, but Ima2p overexpression allows
the ima1 null mutant to grow on isomaltose"
/codon_start=1
/product="oligo-1,6-glucosidase IMA2"
/protein_id="NP_014485.1"
/db_xref="GeneID:854008"
/db_xref="SGD:S000005517"
/translation="MTISSAHPETEPKWWKEATIYQIYPASFKDSNNDGWGDMKGIAS
KLEYIKELGADAIWISPFYDSPQDDMGYDIANYEKVWPTYGTNEDCFALIEKTHKLGM
KFITDLVINHCSSEHEWFKESRSSKTNPKRDWFFWRPPKGYDAEGKPIPPNNWRSYFG
GSAWTFDEKTQEFYLRLFCSTQPDLNWENEDCRKAIYESAVGYWLDHGVDGFRIDVGS
LYSKVAGLPDAPVIDENSKWQPSDPFTMNGPRIHEFHQEMNKFIRNRVKDGREIMTVG
EMQHATDETKRLYTSASRHELSELFNFSHTDVGTSPKFRQNLIPYELKDWKVALAELF
RYVNGTDCWSTIYLENHDQPRSITRFGDDSPKNRVISGKLLSVLLVSLSGTLYVYQGQ
ELGEINFKNWPIEKYEDVEVRNNYDAIKEEHGENSKEMKRFLEAIALISRDHARTPMQ
WSREEPNAGFSGPNAKPWFYLNESFREGINAEDESKDPNSVLNFWKEALRFRKAHKDI
TVYGYDFEFIDLDNKKLFSFTKKYDNKTLFAALNFSSDSIDFTIPNNSSSFKLEFGNY
PRSEVDASSRTLKPWEGRIYISE"
gene <25273..>26976
/gene="HXT11"
/locus_tag="YOL156W"
/gene_synonym="LGT3"
/db_xref="GeneID:854009"
mRNA <25273..>26976
/gene="HXT11"
/locus_tag="YOL156W"
/gene_synonym="LGT3"
/product="hexose transporter HXT11"
/transcript_id="NM_001183409.1"
/db_xref="GeneID:854009"
CDS 25273..26976
/gene="HXT11"
/locus_tag="YOL156W"
/gene_synonym="LGT3"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:1902341
xylitol transmembrane transport [PMID:26996892]"
/experiment="EXISTENCE:mutant phenotype:GO:0005353
fructose transmembrane transporter activity
[PMID:10618490]"
/experiment="EXISTENCE:mutant phenotype:GO:0005354
galactose transmembrane transporter activity
[PMID:10618490]"
/experiment="EXISTENCE:mutant phenotype:GO:0008645 hexose
transmembrane transport [PMID:10618490]"
/experiment="EXISTENCE:mutant phenotype:GO:0015578 mannose
transmembrane transporter activity [PMID:10618490]"
/experiment="EXISTENCE:mutant phenotype:GO:0055056
D-glucose transmembrane transporter activity
[PMID:10618490]"
/note="Hexose transporter; capable of transporting a broad
range of substrates including: glucose, fructose, mannose
and galactose; polyol transporter that supports the growth
on and uptake of xylitol with low affinity when
overexpressed in a strain deleted for hexose family
members; nearly identical in sequence to Hxt9p; has
similarity to major facilitator superfamily (MFS)
transporters; involved in pleiotropic drug resistance"
/codon_start=1
/product="hexose transporter HXT11"
/protein_id="NP_014486.1"
/db_xref="GeneID:854009"
/db_xref="SGD:S000005516"
/translation="MSGVNNTSANELSTTMSNSNSAVGAPSVKTEHGDSKNSLNLDAN
EPPIDLPQKPLSAYTTVAILCLMIAFGGFIFGWDTGTISGFVNLSDFIRRFGQKNDKG
TYYLSKVRMGLIVSIFNIGCAIGGIVLSKVGDIYGRRIGLITVTAIYVVGILIQITSI
NKWYQYFIGRIISGLGVGGIAVLSPMLISEVAPKHIRGTLVQLYQLMGTMGIFLGYCT
NYGTKNYHNATQWRVGLGLCFAWATFMVSGMMFVPESPRYLIEVGKDEEAKRSLSKSN
KVSVDDPALLVEYDTIKAGIELEKLAGNASWSELLSTKTKVFQRVLMGVMIQSLQQLT
GDNYFFYYGTTIFKSVGLKDSFQTSIIIGVVNFFSSFIAVYTIERFGRRTCLLWGAAS
MLCCFAVFASVGVTKLWPQGSSHQDITSQGAGNCMIVFTMFFIFSFATTWAGGCYVIV
SETFPLRVKSRGMAIATAANWMWGFLISFFTPFITGAINFYYGYVFLGCLVFAYFYVF
FFVPETKGLTLEEVNTMWLEGVPAWKSASWVPPERRTADYDADAIDHDNRPIYKRFFS
S"
gene <27084..>27218
/locus_tag="YOL155W-A"
/db_xref="GeneID:1466474"
mRNA <27084..>27218
/locus_tag="YOL155W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184666.1"
/db_xref="GeneID:1466474"
CDS 27084..27218
/locus_tag="YOL155W-A"
/note="hypothetical protein; identified by expression
profiling and mass spectrometry"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878162.1"
/db_xref="GeneID:1466474"
/db_xref="SGD:S000028855"
/translation="MFYGSFNKCVTGYSCRMAIHYYVYRIIKSATRPDYKSNTQILVL
"
gene complement(<28703..>31606)
/gene="HPF1"
/locus_tag="YOL155C"
/db_xref="GeneID:854010"
mRNA complement(<28703..>31606)
/gene="HPF1"
/locus_tag="YOL155C"
/product="mannoprotein"
/transcript_id="NM_001183408.1"
/db_xref="GeneID:854010"
CDS complement(28703..31606)
/gene="HPF1"
/locus_tag="YOL155C"
/experiment="EXISTENCE:direct assay:GO:0005576
extracellular region [PMID:11935221]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:11748726]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0031505
fungal-type cell wall organization [PMID:11748726]"
/note="Haze-protective mannoprotein; reduces particle size
of aggregated proteins in white wines, thereby decreasing
turbidity; intragenic repeat expansion controls
chronological aging; expansion of intragenic tandem
repeats within N-terminus sufficient to cause pronounced
life span shortening; repeat expansion shifts cells from
sedentary to buoyant, thereby increasing exposure to
oxygen, altering methionine, lipid, and purine metabolism"
/codon_start=1
/product="mannoprotein"
/protein_id="NP_014487.1"
/db_xref="GeneID:854010"
/db_xref="SGD:S000005515"
/translation="MFNRFNKLQAALALVLYSQSALGQYYTNSSSIASNSSTAVSSTS
SGSVSISSSIELTSSTSDVSSSLTELTSSSTEVSSSIAPSTSSSEVSSSITSSGSSVS
GSSSITSSGSSVSSSSSATESGSSASGSSSATESGSSVSGSSTSITSGSSSATESGSS
VSGSTSATESGSSASGSSSATESGSSASGSSSATESGSSVSGSSSATESGSSVSGSSS
ATESGSASSVPSSSGSVTESGSSSSASESSITQSGTASGSSASSTSGSVTQSGSSVSG
SSASSAPGISSSIPQSTSSASTASGSITSGTLSSITSSASSATATASNSLSSSDGTIY
LPSTTISGDITLTGSVIATEAVEVAAGGKLTLLDGDKYVFSADFIIHGGVFVEKSKPT
YPGTEFDISGENFDVSGTFNAEEPAASSASAYSFTPGSFDNSGDISLSLSESTKGEVT
FSPYSNSGAFSFSNAILNGGSVSGLQRRAESGSVNNGEINLENGSTYVVVEPVSGSGT
INIISGNLYLHYPDTFTGQTVVFKGEGVLAVDPTETNTTPIPVVGYTGENQIAITADV
TALSYDSATGVLTATQGNSQFSFSIGTGFSSSGFNVSEGTFAGAYAYYLNYGGVVASS
ATPSSTSTTSGATNSTSGSTSFGASVTGSTASTSFGASVTGSTASTLISGSPSVYTTT
LTYATTTSTVVVSCSETTDSNGNVYTITTTVPCSSTTATITSCDETGCHVTTSTGTVA
TETVSSKSYTTVTVTHCDNNGCNTKTVTSECPEETSATTTSPKSYTTVTVTHCDDNGC
NTKTVTSEAPEATTTTVSPKTYTTATVTQCDDNGCSTKTVTSEAPKETSETSETSAAP
KTYTTATVTQCDDNGCNVKIITSQIPEATSTVTATSASPKSYTTVTSEGSKATSLTTA
ISKASSAISTYSKSAAPIKTSTGIIVQSEGIAAGLNANTLNALVGIFVLAFFN"
gene <34658..>35407
/gene="ZPS1"
/locus_tag="YOL154W"
/db_xref="GeneID:854011"
mRNA <34658..>35407
/gene="ZPS1"
/locus_tag="YOL154W"
/product="Zps1p"
/transcript_id="NM_001183407.1"
/db_xref="GeneID:854011"
CDS 34658..35407
/gene="ZPS1"
/locus_tag="YOL154W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:11935221]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="Putative GPI-anchored protein; transcription is
induced under low-zinc conditions, as mediated by the
Zap1p transcription factor, and at alkaline pH"
/codon_start=1
/product="Zps1p"
/protein_id="NP_014488.1"
/db_xref="GeneID:854011"
/db_xref="SGD:S000005514"
/translation="MKFSSGKSIIFATIASLALSAPVTYDTNSTAELQSPSSQEILGW
SHATFPTIYQTCNETNARMLNAAFKDTAEITAYGKDRLLNYGVDDVYYKRWFGNGSIF
TVMGVFEQLMEASKGAMLMRCDDIDGLCAANPNYYAGHHRQSAPAETVICDYFYTSKK
PLSTICFEGTIVDVGPKHYAGIDMLHRYLHVPTMSMDGYVGEYAETLEEVVDYTQNNA
TYAVRNTDNYLYYLADVYSASVIPGGCLGNL"
rep_origin 35669..35904
/note="ARS1531; Autonomously Replicating Sequence"
/db_xref="SGD:S000118995"
gene complement(36822..38567)
/locus_tag="YOL153C"
/pseudo
/db_xref="GeneID:854012"
CDS complement(36822..38567)
/locus_tag="YOL153C"
/note="Blocked reading frame, contains two in-frame stops
in translation; this ORF is conserved, without the stops,
in Saccharomyces paradoxus, S. mikatae, and S. bayanus;
RNA sequencing data indicate the presence of a
transcription unit at this reading frame"
/pseudo
/codon_start=1
/db_xref="GeneID:854012"
/db_xref="SGD:S000005513"
gene <40748..>42610
/gene="FRE7"
/locus_tag="YOL152W"
/db_xref="GeneID:854013"
mRNA <40748..>42610
/gene="FRE7"
/locus_tag="YOL152W"
/product="putative ferric-chelate reductase"
/transcript_id="NM_001183406.1"
/db_xref="GeneID:854013"
CDS 40748..42610
/gene="FRE7"
/locus_tag="YOL152W"
/EC_number="1.16.1.9"
/experiment="EXISTENCE:direct assay:GO:0000293
ferric-chelate reductase activity [PMID:17681937]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:17553781]"
/experiment="EXISTENCE:genetic interaction:GO:0000293
ferric-chelate reductase activity [PMID:17553781]"
/experiment="EXISTENCE:genetic interaction:GO:0006826 iron
ion transport [PMID:17553781]"
/experiment="EXISTENCE:genetic interaction:GO:0015677
copper ion import [PMID:17553781]"
/note="Putative ferric reductase with similarity to Fre2p;
expression induced by low copper levels"
/codon_start=1
/product="putative ferric-chelate reductase"
/protein_id="NP_014489.2"
/db_xref="GeneID:854013"
/db_xref="SGD:S000005512"
/translation="MIEERDLVLSNGIHCIADIHSELYARLKKESQAATPWVYQKQYG
KFVTYFVAVIIFLSLIKKLAFMYYDSSEEFLPEKKNSPTTPSVFLARIMTKLVAFNRY
ICYRKFPTLIFSYLGIPTSVGTFLVVMATTLYTLLYCFVPHPFYRPCAGFGSPPLSVR
AGIMAISLVPFVFSLSGKINVIGWLVGLSYEKINIYHQWASILCLFFSWVHVIPFLRQ
ARHEGGYERMHQRWKASDMWRSGVPPILFLNLLWLSSLPIARRHFYEIFLQLHWILAV
GFYISLFYHVYPELNSHMYLVATIVVWFAQLFYRLAVKGYLRPGRSFMASTIANVSIV
GEGCVELIVKDVEMAYSPGQHIFVRTIDKGIISNHPFSIFPSAKYPGGIKMLIRAQKG
FSKRLYESNDDMKKILIDGPYGGIERDIRSFTNVYLICSGSGISTCLPFLQKYGPILH
KTNLEVITLDWVVRHREDISWIRDEMCTLSNNLRQLFLDGKIVVRIYVCSDSTVPGII
KTFPQTIDTASDQSDLAKREKDTEFGQDDTESNSTFDKSNNEYKGLITIIPSKPDLNQ
VINDYQIGFRNCFICSGSDSLRYTVGNSVAGLQAKVFSNKNVEECYLHSESFGY"
gene <43694..>44722
/gene="GRE2"
/locus_tag="YOL151W"
/db_xref="GeneID:854014"
mRNA <43694..>44722
/gene="GRE2"
/locus_tag="YOL151W"
/product="methylglyoxal reductase (NADPH-dependent) GRE2"
/transcript_id="NM_001183405.1"
/db_xref="GeneID:854014"
CDS 43694..44722
/gene="GRE2"
/locus_tag="YOL151W"
/EC_number="1.1.1.265"
/EC_number="1.1.1.283"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12206772]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:12206772]"
/experiment="EXISTENCE:direct assay:GO:0043892
methylglyoxal reductase (NADPH) activity [PMID:12722185]"
/experiment="EXISTENCE:genetic interaction:GO:0008204
ergosterol metabolic process [PMID:16598690]"
/experiment="EXISTENCE:mutant phenotype:GO:0008204
ergosterol metabolic process [PMID:16598690]"
/experiment="EXISTENCE:mutant phenotype:GO:0030447
filamentous growth [PMID:16999827]"
/experiment="EXISTENCE:mutant phenotype:GO:0046568
3-methylbutanal reductase [NAD(P)H] activity
[PMID:16999827]"
/note="3-methylbutanal reductase and NADPH-dependent
methylglyoxal reductase; stress induced (osmotic, ionic,
oxidative, heat shock and heavy metals); regulated by the
HOG pathway; restores resistance to glycolaldehyde by
coupling reduction of glycolaldehyde to ethylene glycol
and oxidation of NADPH to NADP+; protein abundance
increases in response to DNA replication stress;
methylglyoxal reductase (NADPH-dependent) is also known as
D-lactaldehyde dehydrogenase"
/codon_start=1
/product="methylglyoxal reductase (NADPH-dependent) GRE2"
/protein_id="NP_014490.1"
/db_xref="GeneID:854014"
/db_xref="SGD:S000005511"
/translation="MSVFVSGANGFIAQHIVDLLLKEDYKVIGSARSQEKAENLTEAF
GNNPKFSMEVVPDISKLDAFDHVFQKHGKDIKIVLHTASPFCFDITDSERDLLIPAVN
GVKGILHSIKKYAADSVERVVLTSSYAAVFDMAKENDKSLTFNEESWNPATWESCQSD
PVNAYCGSKKFAEKAAWEFLEENRDSVKFELTAVNPVYVFGPQMFDKDVKKHLNTSCE
LVNSLMHLSPEDKIPELFGGYIDVRDVAKAHLVAFQKRETIGQRLIVSEARFTMQDVL
DILNEDFPVLKGNIPVGKPGSGATHNTLGATLDNKKSKKLLGFKFRNLKETIDDTASQ
ILKFEGRI"
gene <44938..>45633
/gene="DCP1"
/locus_tag="YOL149W"
/gene_synonym="MRT2"
/db_xref="GeneID:854016"
mRNA <44938..>45633
/gene="DCP1"
/locus_tag="YOL149W"
/gene_synonym="MRT2"
/product="Dcp1p"
/transcript_id="NM_001183403.1"
/db_xref="GeneID:854016"
CDS 44938..45633
/gene="DCP1"
/locus_tag="YOL149W"
/gene_synonym="MRT2"
/experiment="EXISTENCE:direct assay:GO:0000290
deadenylation-dependent decapping of nuclear-transcribed
mRNA [PMID:11139489]"
/experiment="EXISTENCE:direct assay:GO:0000932 P-body
[PMID:23509072|PMID:12730603]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:11139489]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:23706738]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:23706738|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0008047 enzyme
activator activity [PMID:16341225]"
/experiment="EXISTENCE:direct assay:GO:0098562 cytoplasmic
side of membrane [PMID:27146487]"
/experiment="EXISTENCE:direct assay:GO:0098745 RNA
decapping complex [PMID:12554866]"
/experiment="EXISTENCE:mutant phenotype:GO:0000932 P-body
[PMID:12730603]"
/experiment="EXISTENCE:physical interaction:GO:0098745 RNA
decapping complex [PMID:10508173]"
/note="Subunit of the Dcp1p-Dcp2p decapping enzyme
complex; decapping complex removes the 5' cap structure
from mRNAs prior to their degradation; enhances the
activity of catalytic subunit Dcp2p; regulated by DEAD box
protein Dhh1p; forms cytoplasmic foci upon DNA replication
stress"
/codon_start=1
/product="Dcp1p"
/protein_id="NP_014492.1"
/db_xref="GeneID:854016"
/db_xref="SGD:S000005509"
/translation="MTGAATAAENSATQLEFYRKALNFNVIGRYDPKIKQLLFHTPHA
SLYKWDFKKDEWNKLEYQGVLAIYLRDVSQNTNLLPVSPQEVDIFDSQNGSNNIQVNN
GSDNSNRNSSGNGNSYKSNDSLTYNCGKTLSGKDIYNYGLIILNRINPDNFSMGIVPN
SVVNKRKVFNAEEDTLNPLECMGVEVKDELVIIKNLKHEVYGIWIHTVSDRQNIYELI
KYLLENEPKDSFA"
gene complement(<45760..>47574)
/gene="SPT20"
/locus_tag="YOL148C"
/gene_synonym="ADA5"
/db_xref="GeneID:854017"
mRNA complement(<45760..>47574)
/gene="SPT20"
/locus_tag="YOL148C"
/gene_synonym="ADA5"
/product="Spt20p"
/transcript_id="NM_001183402.1"
/db_xref="GeneID:854017"
CDS complement(45760..47574)
/gene="SPT20"
/locus_tag="YOL148C"
/gene_synonym="ADA5"
/experiment="EXISTENCE:direct assay:GO:0000124 SAGA
complex [PMID:9674426|PMID:9224714]"
/experiment="EXISTENCE:direct assay:GO:0006325 chromatin
organization [PMID:9674426]"
/experiment="EXISTENCE:direct assay:GO:0046695 SLIK
(SAGA-like) complex [PMID:12446794]"
/experiment="EXISTENCE:mutant phenotype:GO:0003712
transcription coregulator activity [PMID:8649430]"
/experiment="EXISTENCE:mutant phenotype:GO:0036498
IRE1-mediated unfolded protein response [PMID:10652329]"
/note="Subunit of the SAGA transcriptional regulatory
complex; involved in maintaining the integrity of the
complex; mutant displays reduced transcription elongation
in the G-less-based run-on (GLRO) assay"
/codon_start=1
/product="Spt20p"
/protein_id="NP_014493.1"
/db_xref="GeneID:854017"
/db_xref="SGD:S000005508"
/translation="MSANSPTGNDPHVFGIPVNATPSNMGSPGSPVNVPPPMNPAVAN
VNHPVMRTNSNSNANEGTRTLTREQIQQLQQRQRLLLQQRLLEQQRKQQALQNYEAQF
YQMLMTLNKRPKRLYNFVEDADSILKKYEQYLHSFEFHIYENNYKICAPANSRLQQQQ
KQPELTSDGLILTKNNETLKEFLEYVARGRIPDAIMEVLRDCNIQFYEGNLILQVYDH
TNTVDVTPKENKPNLNSSSSPSNNNSTQDNSKIQQPSEPNSGVANTGANTANKKASFK
RPRVYRTLLKPNDLTTYYDMMSYADNARFSDSIYQQFESEILTLTKRNLSLSVPLNPY
EHRDMLEETAFSEPHWDSEKKSFIHEHRAESTREGTKGVVGHIEERDEFPQHSSNYEQ
LMLIMNERTTTITNSTFAVSLTKNAMEIASSSSNGVRGASSSTSNSASNTRNNSLANG
NQVALAAAAAAAAVGSTMGNDNNQFSRLKFIEQWRINKEKRKQQALSANINPTPFNAR
ISMTAPLTPQQQLLQRQQQALEQQQNGGAMKNANKRSGNNATSNNNNNNNNLDKPKVK
RPRKNAKKSESGTPAPKKKRMTKKKQSASSTPSSTTMS"
gene complement(<47933..>48643)
/gene="PEX11"
/locus_tag="YOL147C"
/gene_synonym="PMP24; PMP27"
/db_xref="GeneID:854018"
mRNA complement(<47933..>48643)
/gene="PEX11"
/locus_tag="YOL147C"
/gene_synonym="PMP24; PMP27"
/product="Pex11p"
/transcript_id="NM_001183401.1"
/db_xref="GeneID:854018"
CDS complement(47933..48643)
/gene="PEX11"
/locus_tag="YOL147C"
/gene_synonym="PMP24; PMP27"
/experiment="EXISTENCE:direct assay:GO:0005778 peroxisomal
membrane [PMID:7721939|PMID:7860627]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:20028986]"
/experiment="EXISTENCE:direct assay:GO:1990429 peroxisomal
importomer complex [PMID:22375831]"
/experiment="EXISTENCE:mutant phenotype:GO:0001579
medium-chain fatty acid transport [PMID:10931862]"
/experiment="EXISTENCE:mutant phenotype:GO:0016559
peroxisome fission [PMID:7721939|PMID:7860627]"
/note="Peroxisomal protein required for medium-chain fatty
acid oxidation; also required for peroxisome
proliferation, possibly by inducing membrane curvature;
localization regulated by phosphorylation; transcription
regulated by Adr1p and Pip2p-Oaf1p"
/codon_start=1
/product="Pex11p"
/protein_id="NP_014494.1"
/db_xref="GeneID:854018"
/db_xref="SGD:S000005507"
/translation="MVCDTLVYHPSVTRFVKFLDGSAGREKVLRLLQYLARFLAVQNS
SLLARQLQAQFTTVRKFLRFLKPLNHLQAAAKFYDNKLASDNVVRVCNVLKNIFFAAY
LSLDQVNLLRILKVIPVTVLTGKKIPRWSNWCWLFGLLSGLAMDLRKIQTSHAQIAAF
VKAKSQSQGDEHEDHKKVLGKAYQDRYTALRRLFWDAADSFIVLNNLGYLSSNEEYVA
LSGVVTSILGMQDMWKAT"
gene <48864..>49448
/gene="PSF3"
/locus_tag="YOL146W"
/db_xref="GeneID:854019"
mRNA <48864..>49448
/gene="PSF3"
/locus_tag="YOL146W"
/product="DNA replication protein PSF3"
/transcript_id="NM_001183400.1"
/db_xref="GeneID:854019"
CDS 48864..49448
/gene="PSF3"
/locus_tag="YOL146W"
/experiment="EXISTENCE:direct assay:GO:0071162 CMG complex
[PMID:26524492]"
/experiment="EXISTENCE:genetic interaction:GO:0006261
DNA-templated DNA replication [PMID:12730134]"
/experiment="EXISTENCE:mutant phenotype:GO:0000727
double-strand break repair via break-induced replication
[PMID:20516198]"
/experiment="EXISTENCE:physical interaction:GO:0000811
GINS complex [PMID:12730134|PMID:12768207]"
/experiment="EXISTENCE:physical interaction:GO:0031261 DNA
replication preinitiation complex [PMID:12730134]"
/note="Subunit of the GINS complex (Sld5p, Psf1p, Psf2p,
Psf3p); complex is localized to DNA replication origins
and implicated in assembly of the DNA replication
machinery"
/codon_start=1
/product="DNA replication protein PSF3"
/protein_id="NP_014495.2"
/db_xref="GeneID:854019"
/db_xref="SGD:S000005506"
/translation="MGYYDIDDVLADGTEFPCKFQYDIPGLGYLENNPGRPITKNTKL
SLPLWLARILAIVGGDEALVDEEPVPFVELLPPDMFSTKVMNAIKTDPVALDLHSINS
HFFSLAIKWIMLFSEKELANVVSELLLQRAQELNHHASSLSIDLNADSTGKNSANTNI
ATSTFLLKLEEMEKEIYKKSHESYKDTKRWMFKK"
gene complement(<49555..>52788)
/gene="CTR9"
/locus_tag="YOL145C"
/gene_synonym="CDP1"
/db_xref="GeneID:854020"
mRNA complement(<49555..>52788)
/gene="CTR9"
/locus_tag="YOL145C"
/gene_synonym="CDP1"
/product="Ctr9p"
/transcript_id="NM_001183399.2"
/db_xref="GeneID:854020"
CDS complement(49555..52788)
/gene="CTR9"
/locus_tag="YOL145C"
/gene_synonym="CDP1"
/experiment="EXISTENCE:direct assay:GO:0000791 euchromatin
[PMID:15531585]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:15643076|PMID:10684247|PMID:10219085]"
/experiment="EXISTENCE:direct assay:GO:0045142 triplex DNA
binding [PMID:11058104]"
/experiment="EXISTENCE:direct assay:GO:1990269 RNA
polymerase II C-terminal domain phosphoserine binding
[PMID:22796944]"
/experiment="EXISTENCE:direct assay:GO:2001209 positive
regulation of transcription elongation by RNA polymerase I
[PMID:20299458]"
/experiment="EXISTENCE:genetic interaction:GO:0006360
transcription by RNA polymerase I [PMID:20299458]"
/experiment="EXISTENCE:genetic interaction:GO:0006368
transcription elongation by RNA polymerase II
[PMID:11927560]"
/experiment="EXISTENCE:genetic interaction:GO:0090262
regulation of transcription-coupled nucleotide-excision
repair [PMID:21737840]"
/experiment="EXISTENCE:mutant phenotype:GO:0000082 G1/S
transition of mitotic cell cycle [PMID:10219085]"
/experiment="EXISTENCE:mutant phenotype:GO:0001015 snoRNA
transcription by RNA polymerase II [PMID:16246725]"
/experiment="EXISTENCE:mutant phenotype:GO:0006353
DNA-templated transcription termination [PMID:23109428]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:10219085]"
/experiment="EXISTENCE:mutant phenotype:GO:0006362
transcription elongation by RNA polymerase I
[PMID:19164765]"
/experiment="EXISTENCE:mutant phenotype:GO:0009302
sno(s)RNA transcription [PMID:23109428]"
/experiment="EXISTENCE:mutant phenotype:GO:0031124 mRNA
3'-end processing [PMID:15149594]"
/experiment="EXISTENCE:mutant phenotype:GO:0031126
sno(s)RNA 3'-end processing [PMID:16246725]"
/experiment="EXISTENCE:mutant phenotype:GO:0060260
regulation of transcription initiation by RNA polymerase
II [PMID:18194564]"
/experiment="EXISTENCE:mutant phenotype:GO:1901525
negative regulation of mitophagy [PMID:31525119]"
/experiment="EXISTENCE:physical interaction:GO:0000993 RNA
polymerase II complex binding [PMID:11884586]"
/experiment="EXISTENCE:physical interaction:GO:0003712
transcription coregulator activity [PMID:11927560]"
/experiment="EXISTENCE:physical interaction:GO:0016593
Cdc73/Paf1 complex
[PMID:11884586|PMID:11927560|PMID:10219085]"
/experiment="EXISTENCE:physical interaction:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:11927560]"
/note="Component of the Paf1p complex involved in
transcription elongation; binds to and modulates the
activity of RNA polymerases I and II; required for
expression of a subset of genes, including cyclin genes;
involved in SER3 repression by helping to maintain SRG1
transcription-dependent nucleosome occupancy; contains TPR
repeats"
/codon_start=1
/product="Ctr9p"
/protein_id="NP_014496.2"
/db_xref="GeneID:854020"
/db_xref="SGD:S000005505"
/translation="MTNAMKVEGYPSMEWPTSLDIPLKASEELVGIDLETDLPDDPTD
LKTLLVEENSEKEHWLTIALAYCNHGKTNEGIKLIEMALDVFQNSERASLHTFLTWAH
LNLAKGQSLSVETKEHELTQAELNLKDAIGFDPTWIGNMLATVELYYQRGHYDKALET
SDLFVKSIHAEDHRSGRQSKPNCLFLLLRAKLLYQKKNYMASLKIFQELLVINPVLQP
DPRIGIGLCFWQLKDSKMAIKSWQRALQLNPKNTSASILVLLGEFRESFTNSTNDKTF
KEAFTKALSDLNNIFSENQHNPVLLTLLQTYYYFKGDYQTVLDIYHHRILKMSPMIAK
IVLSESSFWCGRAHYALGDYRKSFIMFQESLKKNEDNLLAKLGLGQTQIKNNLLEESI
ITFENLYKTNESLQELNYILGMLYAGKAFDAKTAKNTSAKEQSNLNEKALKYLERYLK
LTLATKNQLVISRAYLVISQLYELQNQYKTSLDYLSKALEEMEFIKKEIPLEVLNNLA
CYHFINGDFIKADDLFKQAKAKVSDKDESVNITLEYNIARTNEKNDCEKSESIYSQVT
SLHPAYIAARIRNLYLKFAQSKIEDSDMSTEMNKLLDLNKSDLEIRSFYGWYLKNSKE
RKNNEKSTTHNKETLVKYNSHDAYALISLANLYVTIARDGKKSRNPKEQEKSKHSYLK
AIQLYQKVLQVDPFNIFAAQGLAIIFAESKRLGPALEILRKVRDSLDNEDVQLNLAHC
YLEMREYGKAIENYELVLKKFDNEKTRPHILNLLGRAWYARAIKERSVNFYQKALENA
KTALDLFVKESSKSKFIHSVKFNIALLHFQIAETLRRSNPKFRTVQQIKDSLEGLKEG
LELFRELNDLKEFNMIPKEELEQRIQLGETTMKSALERSLNEQEEFEKEQSAKIDEAR
KILEENELKEQGWMKQEEEARRLKLEKQAEEYRKLQDEAQKLIQEREAMAISEHNVKD
DSDLSDKDNEYDEEKPRQKRKRSTKTKNSGESKRRKAAKKTLSDSDEDDDDVVKKPSH
NKGKKSQLSNEFIEDSDEEEAQMSGSEQNKNDDNDENNDNDDNDGLF"
gene <53098..>54552
/gene="NOP8"
/locus_tag="YOL144W"
/db_xref="GeneID:854021"
mRNA <53098..>54552
/gene="NOP8"
/locus_tag="YOL144W"
/product="Nop8p"
/transcript_id="NM_001183398.1"
/db_xref="GeneID:854021"
CDS 53098..54552
/gene="NOP8"
/locus_tag="YOL144W"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:9891085]"
/experiment="EXISTENCE:direct assay:GO:0008428
ribonuclease inhibitor activity [PMID:21747919]"
/experiment="EXISTENCE:mutant phenotype:GO:0000463
maturation of LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:9891085]"
/experiment="EXISTENCE:mutant phenotype:GO:0042273
ribosomal large subunit biogenesis [PMID:9891085]"
/note="Nucleolar protein required for 60S ribosomal
subunit biogenesis"
/codon_start=1
/product="Nop8p"
/protein_id="NP_014497.1"
/db_xref="GeneID:854021"
/db_xref="SGD:S000005504"
/translation="MDSVIQKRIFVGNIFHNADDCYSELLDRFGKFGDCQDFQFEKHN
HFAFIDIRFNDEADFNKLRKSFNNVKFKGNILKVDEAKPNWESTWAVQHAKDLKEDII
LNAKMKKKNWQHYKKMENVAKSWKDHKEVIAGRMREAPRKRSQLRNITFRINVNGSLK
VYKCYKTKLWGYERNKELNDLVYKFTNNFWKNGYNHIVDRLDYSRAVKTVRFKNGLKQ
LTVSKDENVCSGEMDSDENMSEEEKEKNNVILNDLLKDFDFDKPMTLNDSDEELLTEQ
RKGEEEEEEEEEKEVNAPEYENVNKTKDQSTLPQEKPEERKEQDEGDGQEDNEFIPTF
TKEIGQGTISNTETLRNLFNPNEAEPVSQFKLIEDSDNDIDHAKDVDVNQLEEEVSKS
SDTLGLTSAPVPHVSRDKDNKNFLFFPHLQSPFLVGQTQLSKVRAPGRETMLSNWDEE
FWANRGNWTRDMRRKMKDALKHRKRKQSKSGLLL"
gene complement(<54595..>55104)
/gene="RIB4"
/locus_tag="YOL143C"
/db_xref="GeneID:854022"
mRNA complement(<54595..>55104)
/gene="RIB4"
/locus_tag="YOL143C"
/product="lumazine synthase RIB4"
/transcript_id="NM_001183397.1"
/db_xref="GeneID:854022"
CDS complement(54595..55104)
/gene="RIB4"
/locus_tag="YOL143C"
/EC_number="2.5.1.78"
/experiment="EXISTENCE:direct assay:GO:0000906
6,7-dimethyl-8-ribityllumazine synthase activity
[PMID:7559556]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005758
mitochondrial intermembrane space [PMID:22984289]"
/experiment="EXISTENCE:mutant phenotype:GO:0009231
riboflavin biosynthetic process
[PMID:5366000|PMID:7559556]"
/note="Lumazine synthase (DMRL synthase); catalyzes
synthesis of immediate precursor to riboflavin; DMRL
synthase stands for 6,7-dimethyl-8-ribityllumazine
synthase"
/codon_start=1
/product="lumazine synthase RIB4"
/protein_id="NP_014498.1"
/db_xref="GeneID:854022"
/db_xref="SGD:S000005503"
/translation="MAVKGLGKPDQVYDGSKIRVGIIHARWNRVIIDALVKGAIERMA
SLGVEENNIIIETVPGSYELPWGTKRFVDRQAKLGKPLDVVIPIGVLIKGSTMHFEYI
SDSTTHALMNLQEKVDMPVIFGLLTCMTEEQALARAGIDEAHSMHNHGEDWGAAAVEM
AVKFGKNAF"
gene <55558..>56280
/gene="RRP40"
/locus_tag="YOL142W"
/gene_synonym="MTR14"
/db_xref="GeneID:854023"
mRNA <55558..>56280
/gene="RRP40"
/locus_tag="YOL142W"
/gene_synonym="MTR14"
/product="exosome non-catalytic core subunit RRP40"
/transcript_id="NM_001183396.2"
/db_xref="GeneID:854023"
CDS 55558..56280
/gene="RRP40"
/locus_tag="YOL142W"
/gene_synonym="MTR14"
/experiment="EXISTENCE:direct assay:GO:0000176 nuclear
exosome (RNase complex) [PMID:10465791|PMID:19046973]"
/experiment="EXISTENCE:direct assay:GO:0000177 cytoplasmic
exosome (RNase complex) [PMID:10465791|PMID:19046973]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:17391830]"
/experiment="EXISTENCE:direct assay:GO:0030145 manganese
ion binding [PMID:17159918]"
/experiment="EXISTENCE:direct assay:GO:0071038
TRAMP-dependent tRNA surveillance pathway
[PMID:15828860|PMID:17643380]"
/experiment="EXISTENCE:mutant phenotype:GO:0000467
exonucleolytic trimming to generate mature 3'-end of 5.8S
rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S
rRNA, LSU-rRNA) [PMID:10508172|PMID:10465791]"
/experiment="EXISTENCE:mutant phenotype:GO:0071035 nuclear
polyadenylation-dependent rRNA catabolic process
[PMID:10465791]"
/note="Exosome non-catalytic core component; involved in
3'-5' RNA processing and degradation in both the nucleus
and the cytoplasm; predicted to contain both S1 and KH RNA
binding domains; mutations in the human homolog, EXOSC3,
cause pontocerebellar hypoplasia with motor neuron
degeneration"
/codon_start=1
/product="exosome non-catalytic core subunit RRP40"
/protein_id="NP_014499.2"
/db_xref="GeneID:854023"
/db_xref="SGD:S000005502"
/translation="MSTFIFPGDSFPVDPTTPVKLGPGIYCDPNTQEIRPVNTGVLHV
SAKGKSGVQTAYIDYSSKRYIPSVNDFVIGVIIGTFSDSYKVSLQNFSSSVSLSYMAF
PNASKKNRPTLQVGDLVYARVCTAEKELEAEIECFDSTTGRDAGFGILEDGMIIDVNL
NFARQLLFNNDFPLLKVLAAHTKFEVAIGLNGKIWVKCEELSNTLACYRTIMECCQKN
DTAAFKDIAKRQFKEILTVKEE"
gene <56452..>58539
/gene="PPM2"
/locus_tag="YOL141W"
/gene_synonym="TYW4"
/db_xref="GeneID:854024"
mRNA <56452..>58539
/gene="PPM2"
/locus_tag="YOL141W"
/gene_synonym="TYW4"
/product="tRNA methyltransferase PPM2"
/transcript_id="NM_001183395.2"
/db_xref="GeneID:854024"
CDS 56452..58539
/gene="PPM2"
/locus_tag="YOL141W"
/gene_synonym="TYW4"
/EC_number="2.1.1.290"
/EC_number="2.3.1.231"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0008175 tRNA
methyltransferase activity [PMID:16642040]"
/experiment="EXISTENCE:direct assay:GO:0030488 tRNA
methylation [PMID:16642040]"
/experiment="EXISTENCE:mutant phenotype:GO:0008175 tRNA
methyltransferase activity [PMID:16642040]"
/experiment="EXISTENCE:mutant phenotype:GO:0030488 tRNA
methylation [PMID:16642040]"
/experiment="EXISTENCE:mutant phenotype:GO:0031591
wybutosine biosynthetic process
[PMID:16642040|PMID:17150819]"
/note="AdoMet-dependent tRNA methyltransferase; also
involved in methoxycarbonylation; required for the
synthesis of wybutosine (yW), a modified guanosine found
at the 3'-position adjacent to the anticodon of phe-tRNA;
similarity to Ppm1p"
/codon_start=1
/product="tRNA methyltransferase PPM2"
/protein_id="NP_014500.2"
/db_xref="GeneID:854024"
/db_xref="SGD:S000005501"
/translation="MKNLTTIKQTNKNVKQERRKKYADLAIQGTNNSSIASKRSVELL
YLPKLSSANNFQMDKNNKLLEYFKFFVPKKIKRSPCINRGYWLRLFAIRSRLNSIIEQ
TPQDKKIVVVNLGCGYDPLPFQLLDTNNIQSQQYHDRVSFIDIDYSDLLKIKIELIKT
IPELSKIIGLSEDKDYVDDSNVDFLTTPKYLARPCDLNDSKMFSTLLNECQLYDPNVV
KVFVAEVSLAYMKPERSDSIIEATSKMENSHFIILEQLIPKGPFEPFSKQMLAHFKRN
DSPLQSVLKYNTIESQVQRFNKLGFAYVNVGDMFQLWESADEATKKELLKVEPFDELE
EFHLFCHHYVLCHATNYKEFAFTQGFLFDRSISEINLTVDEDYQLLECECPINRKFGD
VDVAGNDVFYMGGSNPYRVNEILQLSIHYDKIDMKNIEVSSSEVPVARMCHTFTTISR
NNQLLLIGGRKAPHQGLSDNWIFDMKTREWSMIKSLSHTRFRHSACSLPDGNVLILGG
VTEGPAMLLYNVTEEIFKDVTPKDEFFQNSLVSAGLEFDPVSKQGIILGGGFMDQTTV
SDKAIIFKYDAENATEPITVIKKLQHPLFQRYGSQIKYITPRKLLIVGGTSPSGLFDR
TNSIISLDPLSETLTSIPISRRIWEDHSLMLAGFSLVSTSMGTIHIIGGGATCYGFGS
VTNVGLKLIAIAK"
misc_feature 58541..58563
/note="ETC2; Chromosome-organizing-clamp; tethers
chromosomal regions to the nuclear periphery; binds TFIIIC
transcription factor but does not recruit RNA Polymerase
III; can act as a transcription-blocking insulator or as a
heterochromatin barrier element; remains predominantly
localized to the nuclear periphery throughout cell cycle;
located between PPM2 and ARG8"
/db_xref="SGD:S000077073"
gene <58759..>60030
/gene="ARG8"
/locus_tag="YOL140W"
/db_xref="GeneID:854025"
mRNA <58759..>60030
/gene="ARG8"
/locus_tag="YOL140W"
/product="acetylornithine transaminase"
/transcript_id="NM_001183394.1"
/db_xref="GeneID:854025"
CDS 58759..60030
/gene="ARG8"
/locus_tag="YOL140W"
/EC_number="2.6.1.11"
/experiment="EXISTENCE:direct assay:GO:0003992
N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase
activity [PMID:2199330]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:205532]"
/note="Acetylornithine aminotransferase; catalyzes the
fourth step in the biosynthesis of the arginine precursor
ornithine"
/codon_start=1
/product="acetylornithine transaminase"
/protein_id="NP_014501.1"
/db_xref="GeneID:854025"
/db_xref="SGD:S000005500"
/translation="MFKRYLSSTSSRRFTSILEEKAFQVTTYSRPEDLCITRGKNAKL
YDDVNGKEYIDFTAGIAVTALGHANPKVAEILHHQANKLVHSSNLYFTKECLDLSEKI
VEKTKQFGGQHDASRVFLCNSGTEANEAALKFAKKHGIMKNPSKQGIVAFENSFHGRT
MGALSVTWNSKYRTPFGDLVPHVSFLNLNDEMTKLQSYIETKKDEIAGLIVEPIQGEG
GVFPVEVEKLTGLKKICQDNDVIVIHDEIQCGLGRSGKLWAHAYLPSEAHPDIFTSAK
ALGNGFPIAATIVNEKVNNALRVGDHGTTYGGNPLACSVSNYVLDTIADEAFLKQVSK
KSDILQKRLREIQAKYPNQIKTIRGKGLMLGAEFVEPPTEVIKKARELGLLIITAGKS
TVRFVPALTIEDELIEEGMDAFEKAIEAVYA"
gene complement(<60383..>61024)
/gene="CDC33"
/locus_tag="YOL139C"
/gene_synonym="TIF45"
/db_xref="GeneID:854026"
mRNA complement(<60383..>61024)
/gene="CDC33"
/locus_tag="YOL139C"
/gene_synonym="TIF45"
/product="translation initiation factor eIF4E"
/transcript_id="NM_001183393.1"
/db_xref="GeneID:854026"
CDS complement(60383..61024)
/gene="CDC33"
/locus_tag="YOL139C"
/gene_synonym="TIF45"
/experiment="EXISTENCE:direct assay:GO:0000184
nuclear-transcribed mRNA catabolic process,
nonsense-mediated decay [PMID:15753296]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:8119957]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:8119957]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule
[PMID:17908917|PMID:18981231|PMID:26777405]"
/experiment="EXISTENCE:direct assay:GO:0016281 eukaryotic
translation initiation factor 4F complex [PMID:15753296]"
/experiment="EXISTENCE:direct assay:GO:0032266
phosphatidylinositol-3-phosphate binding [PMID:16343487]"
/experiment="EXISTENCE:direct assay:GO:0098808 mRNA cap
binding [PMID:24183571]"
/experiment="EXISTENCE:direct assay:GO:1901195 positive
regulation of formation of translation preinitiation
complex [PMID:30281017]"
/experiment="EXISTENCE:mutant phenotype:GO:0051726
regulation of cell cycle [PMID:2540596]"
/note="Translation initiation factor eIF4E; mRNA cap
binding protein and subunit of the mRNA cap-binding
complex (eIF4F) along with eIF4A and eIF4G, that mediates
cap-dependent translation; required with eIF4G for
Ded1p-mediated stimulation of mRNA recruitment during 48S
preinitiation complex (PIC) assembly; connects protein
synthesis to metabolic gene regulation; protein abundance
increases in response to DNA replication stress; human
homolog EIF4E can complement cdc33 mutants"
/codon_start=1
/product="translation initiation factor eIF4E"
/protein_id="NP_014502.1"
/db_xref="GeneID:854026"
/db_xref="SGD:S000005499"
/translation="MSVEEVSKKFEENVSVDDTTATPKTVLSDSAHFDVKHPLNTKWT
LWYTKPAVDKSESWSDLLRPVTSFQTVEEFWAIIQNIPEPHELPLKSDYHVFRNDVRP
EWEDEANAKGGKWSFQLRGKGADIDELWLRTLLAVIGETIDEDDSQINGVVLSIRKGG
NKFALWTKSEDKEPLLRIGGKFKQVLKLTDDGHLEFFPHSSANGRHPQPSITL"
gene complement(<61325..>65350)
/gene="RTC1"
/locus_tag="YOL138C"
/gene_synonym="SEA2"
/db_xref="GeneID:853982"
mRNA complement(<61325..>65350)
/gene="RTC1"
/locus_tag="YOL138C"
/gene_synonym="SEA2"
/product="Rtc1p"
/transcript_id="NM_001183392.2"
/db_xref="GeneID:853982"
CDS complement(61325..65350)
/gene="RTC1"
/locus_tag="YOL138C"
/gene_synonym="SEA2"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0035859
Seh1-associated complex [PMID:21454883]"
/experiment="EXISTENCE:genetic interaction:GO:1904263
positive regulation of TORC1 signaling [PMID:23716719]"
/note="Subunit of SEACAT, a subcomplex of the SEA complex;
Rtc1p, along with Mtc5p and Sea4p, redundantly inhibit the
TORC1 inhibitory role of the Iml1p/SEACIT
(Iml1p-Npr2p-Npr3p) subcomplex, a GAP for GTPase Gtr1p
(EGOC subunit) in response to amino acid limitation,
thereby resulting in activation of TORC1 signaling; SEA is
a coatomer-related complex that associates dynamically
with the vacuole; has N-terminal WD-40 repeats and a
C-terminal RING motif; null suppresses cdc13-1"
/codon_start=1
/product="Rtc1p"
/protein_id="NP_014503.2"
/db_xref="GeneID:853982"
/db_xref="SGD:S000005498"
/translation="MSLSPHVENASIPKGSTPIPKNRNVSSIGKGEFLGSSSSNNSSF
RMNHYSNSGQPSVLDSIRRPNLTPTFSYSNGVYMPESHRTSSFNDSYLPYDKNPYAKT
TGSMSNKSNMKIKTKKNAINTNTRKSSGLIYTTKVDKELSSIDKVNDPNINGLVCAGK
THLGLYKFSPSDRSIKCVHDFITPNSNTSTRGTTSLLPKLSKRTRQNKFSTIADVKTG
FNNYKNCIAVCNNSTAISIYDLNKSSSIDNPLITSLCEHTRSINSFDFNMVESNLIIS
GGQDSCVKIWDLRSNKSKSSNRSDISINTASDSIRDVKWMPGYNFASKNDQGSSTYGN
LKSGYKFASIHDSGYLLKFDLRQPAQYEKKLNAHTGPGLCLNWHPNQEYIATGGRDGK
CCLWFVGDNANAAENTVLNYGNSPSLHAPNTSLNNSGSLAFPKLTINTGYPVTKLKFK
PAYSSNIYNSLLGISSMGDEAEVRIYSLARKYIPKHVLLSETPSLGLVWWDENLIFNI
DKGTRINGWDINKEPTVLENLSKNTTTWRDLDGNGLLSVDQEIGSYEVVEPELQPTSS
TTCKKHPGTIKNPKNGNPENQGIIGGIKKGFSHTGLTSFTPERPPTLKAGPTFSTKSL
TLASGASSFNSSSASLTSLTPQTENREEIAIEPPCIITLDIPQIFNNIRLTKIAHSRK
KNVISESSSMKNSPVEKFKYLARQLKFSYIREHNVSDSADTAYKNDIENIDVVKNATE
THGDNTTTTNNNDDGDDDDDDDDDDKIIESHLLKKYNFPENNTWATLMNEKVNNKKSK
RNSSSSREFDEKDVRSSISSISASRQSHDRARKIDKNVEAELQEKIQTLVDLISIATH
NASVYLSIDDLTNFKIWILIRDSLLWDLKWMTSSQISSDNASNMDANESSDFEAGENL
KTGKEFPEEDGAGTSGAESLVEERPQAFRANSDEPSDAEKKPVSKLKEQLKNTEIIPY
AQPNEDSDEVLTKLKELQNQRLESRTKMGETVSDDVIIEEDEHEHQEEEQPHDSPTKS
AQFHASPIAKSIPILQKREHRKSFIDTFMLHSPNGYNGDTDIGNEDDNISPRFTYNSV
SPRSKVSSLQSYATTTSQLETFKKLSSHTAPIIGSPRHAPSRPDSIGREQLSSSLTKK
LAKCKKIIADPPWDTKKLIKQLYNQATETGNVVLTVNILFLFQTIYQITEIDIAKDAI
AHFLLLLHRYELFGIAADVLKYCPFEDIMGSEGDQSSIRLFCERCGELITNESSKEKL
RAEAQQTGNKKIMDKFGYWYCDSCKKKNTSCVLCERPLKKLTMVILPCGHEGHFQCIQ
EWFLDENEQECPGGCPGVAFI"
gene <65621..>67114
/gene="BSC6"
/locus_tag="YOL137W"
/db_xref="GeneID:853983"
mRNA <65621..>67114
/gene="BSC6"
/locus_tag="YOL137W"
/product="Bsc6p"
/transcript_id="NM_001183391.1"
/db_xref="GeneID:853983"
CDS 65621..67114
/gene="BSC6"
/locus_tag="YOL137W"
/experiment="EXISTENCE:direct assay:GO:0030136
clathrin-coated vesicle [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0030137 COPI-coated
vesicle [PMID:14562095]"
/note="hypothetical protein with 8 putative transmembrane
segments; ORF exhibits genomic organization compatible
with a translational readthrough-dependent mode of
expression"
/codon_start=1
/product="Bsc6p"
/protein_id="NP_014504.1"
/db_xref="GeneID:853983"
/db_xref="SGD:S000005497"
/translation="MDASSVPPKVDDYGMYTTEISHHNPIELKNLLSSSDSRRNSQDE
DSLPNNTNLIKEIDWQGEKVKTYPLNYQTVPLVKLQVIACLIMFVVFGMNDQTVGALL
PTLIEYYHISRVDVSNVFIVQLCGYVMASLSKERLNKHFGMRGGMLLAAGLCIVFLII
LATAPSSFYVCMFCGLPLGLGIGILDSTGNVLMGSLLVHKNELMGIMHGLYGAAAMVT
PPLVSYFVEWGHWSLFFLIPLFFSIIGMIVIFPAFKFETASKYDYLCSVENKESNNDV
EEAGDNSLMESTKASPGFFELLRNPAIFLYSLYLFLYLGAEITTGSWFFSYLLETKSS
NKVAMSYIAASFWTGLTVGRLCLGFVTERFFENEYKASKAYAFLTLSSYTLFVLVGLI
NSSSVFYFVVLFFVVFCCGTFIGPLFPNASIVALQVLPKRLHVSGVGVAVAVGGCGGA
AIPYLAGVIAHTVGIQYIPLLCWIMVALFTLEWTLYPKFIKGHEEYF"
gene complement(<67561..>68754)
/gene="PFK27"
/locus_tag="YOL136C"
/db_xref="GeneID:853984"
mRNA complement(<67561..>68754)
/gene="PFK27"
/locus_tag="YOL136C"
/product="6-phosphofructo-2-kinase"
/transcript_id="NM_001183390.1"
/db_xref="GeneID:853984"
CDS complement(67561..68754)
/gene="PFK27"
/locus_tag="YOL136C"
/EC_number="2.7.1.105"
/experiment="EXISTENCE:curator inference:GO:0005737
cytoplasm [PMID:8861205]"
/experiment="EXISTENCE:mutant phenotype:GO:0006003
fructose 2,6-bisphosphate metabolic process
[PMID:8861205]"
/experiment="EXISTENCE:mutant phenotype:GO:0006110
regulation of glycolytic process [PMID:8861205]"
/note="6-phosphofructo-2-kinase; catalyzes synthesis of
fructose-2,6-bisphosphate; inhibited by
phosphoenolpyruvate and sn-glycerol 3-phosphate,
expression induced by glucose and sucrose, transcriptional
regulation involves protein kinase A"
/codon_start=1
/product="6-phosphofructo-2-kinase"
/protein_id="NP_014505.1"
/db_xref="GeneID:853984"
/db_xref="SGD:S000005496"
/translation="MGGSSDSDSHDGYLTSEYNSSNSLFSLNTGNSYSSASLDRATLD
CQDSVFFDNHKSSLLSTEVPRFISNDPLHLPITLNYKRDNADPTYTNGKVNKFMIVLI
GLPATGKSTISSHLIQCLKNNPLTNSLRCKVFNAGKIRRQISCATISKPLLLSNTSSE
DLFNPKNNDKKETYARITLQKLFHEINNDECDVGIFDATNSTIERRRFIFEEVCSFNT
DELSSFNLVPIILQVSCFNRSFIKYNIHNKSFNEDYLDKPYELAIKDFAKRLKHYYSQ
FTPFSLDEFNQIHRYISQHEEIDTSLFFFNVINAGVVEPHSLNQSHYPSTCGKQIRDT
IMVIENFINHYSQMFGFEYIEAVKLFFESFGNSSEETLTTLDSVVNDKFFDDLQSLIE
SNGFA"
gene complement(<69376..>70044)
/gene="MED7"
/locus_tag="YOL135C"
/db_xref="GeneID:853985"
mRNA complement(<69376..>70044)
/gene="MED7"
/locus_tag="YOL135C"
/product="mediator complex subunit MED7"
/transcript_id="NM_001183389.1"
/db_xref="GeneID:853985"
CDS complement(69376..70044)
/gene="MED7"
/locus_tag="YOL135C"
/experiment="EXISTENCE:direct assay:GO:0003713
transcription coactivator activity [PMID:19057509]"
/experiment="EXISTENCE:direct assay:GO:0070847 core
mediator complex [PMID:9891034]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:16452140]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:16452140]"
/note="Subunit of the RNA polymerase II mediator complex;
associates with core polymerase subunits to form the RNA
polymerase II holoenzyme; essential for transcriptional
regulation"
/codon_start=1
/product="mediator complex subunit MED7"
/protein_id="NP_014506.1"
/db_xref="GeneID:853985"
/db_xref="SGD:S000005495"
/translation="MSNDPGNEVSSLYPPPPPYVKFFTQSNLEKLPKYKEKKAASAKQ
TAPNNSNGGSEEEITCALDYLIPPPMPKNQQYRAFGSIWQVKDQLPDLESMGLTQLYK
KSTENESTNYQYKIQELRKLLKSLLLNYLELIGVLSINPDMYERKVENIRTILVNIHH
LLNEYRPHQSRESLIMLLEEQLEYKRGEIREIEQVCKQVHDKLTSIQDTLRTGSQSPP
SSSQ"
gene <70325..>70690
/gene="HRT1"
/locus_tag="YOL133W"
/gene_synonym="HRT2; RBX1; ROC1"
/db_xref="GeneID:853986"
mRNA <70325..>70690
/gene="HRT1"
/locus_tag="YOL133W"
/gene_synonym="HRT2; RBX1; ROC1"
/product="SCF ubiquitin ligase complex subunit HRT1"
/transcript_id="NM_001183387.1"
/db_xref="GeneID:853986"
CDS 70325..70690
/gene="HRT1"
/locus_tag="YOL133W"
/gene_synonym="HRT2; RBX1; ROC1"
/experiment="EXISTENCE:direct assay:GO:0004842
ubiquitin-protein transferase activity [PMID:10385629]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11080155]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11080155]"
/experiment="EXISTENCE:direct assay:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:10385629]"
/experiment="EXISTENCE:direct assay:GO:0019005 SCF
ubiquitin ligase complex [PMID:10385629|PMID:19882662]"
/experiment="EXISTENCE:direct assay:GO:0030674
protein-macromolecule adaptor activity [PMID:10213692]"
/experiment="EXISTENCE:direct assay:GO:0031146
SCF-dependent proteasomal ubiquitin-dependent protein
catabolic process [PMID:10385629]"
/experiment="EXISTENCE:direct assay:GO:0031463 Cul3-RING
ubiquitin ligase complex [PMID:12676951]"
/experiment="EXISTENCE:direct assay:GO:0035361 Cul8-RING
ubiquitin ligase complex [PMID:12676951]"
/experiment="EXISTENCE:mutant phenotype:GO:0000082 G1/S
transition of mitotic cell cycle [PMID:10230407]"
/experiment="EXISTENCE:mutant phenotype:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:10385629]"
/experiment="EXISTENCE:physical interaction:GO:0019005 SCF
ubiquitin ligase complex [PMID:10385629]"
/note="RING-H2 domain core subunit of multiple ubiquitin
ligase complexes; subunit of Skp1-Cullin-F-box (SCF) that
tethers the Cdc34p (E2) and Cdc53p (cullin) SCF subunits,
and is required for degradation of Gic2p, Far1p, Sic1p and
Cln2p; subunit of the Rtt101p-Mms1p-Mms22p ubiquitin
ligase that stabilizes replication forks after DNA
lesions; subunit of the Cul3p-Elc1p-Ela1p ubiquitin ligase
involved in Rpb1p degradation as part of
transcription-coupled repair"
/codon_start=1
/product="SCF ubiquitin ligase complex subunit HRT1"
/protein_id="NP_014508.1"
/db_xref="GeneID:853986"
/db_xref="SGD:S000005493"
/translation="MSNEVDRMDVDEDESQNIAQSSNQSAPVETKKKRFEIKKWTAVA
FWSWDIAVDNCAICRNHIMEPCIECQPKAMTDTDNECVAAWGVCNHAFHLHCINKWIK
TRDACPLDNQPWQLARCGR"
gene <71300..>72715
/gene="GAS4"
/locus_tag="YOL132W"
/db_xref="GeneID:853988"
mRNA <71300..>72715
/gene="GAS4"
/locus_tag="YOL132W"
/product="1,3-beta-glucanosyltransferase"
/transcript_id="NM_001183386.1"
/db_xref="GeneID:853988"
CDS 71300..72715
/gene="GAS4"
/locus_tag="YOL132W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:10383953]"
/experiment="EXISTENCE:direct assay:GO:0042124
1,3-beta-glucanosyltransferase activity [PMID:17397106]"
/experiment="EXISTENCE:genetic interaction:GO:0030476
ascospore wall assembly [PMID:17189486]"
/experiment="EXISTENCE:mutant phenotype:GO:0030476
ascospore wall assembly [PMID:17189486]"
/note="1,3-beta-glucanosyltransferase; involved with Gas2p
in spore wall assembly; has similarity to Gas1p; localizes
to the cell wall"
/codon_start=1
/product="1,3-beta-glucanosyltransferase"
/protein_id="NP_014509.1"
/db_xref="GeneID:853988"
/db_xref="SGD:S000005492"
/translation="MMVFSSTFIFLILELVVLCEASVHTIQIKDKHFVDTVTGKPFFI
KGVDYQPGGSSDVSEKQDPLSNPDACARDILLFQELGINTVRIYSINPDLNHDACMTM
LAMAGIYLILDVNSPLQNQHLNRYEPWTTYNEVYLEHVFKVVEQFSHYNNTLGFFAGN
EIVNDKRSAQYSPAYVKELIGTMKNYISAHSPRTIPVGYSAADDLNYRVSLSEYLECK
DDDKPENSVDFYGVNSYQWCGQQTMQTSGYDTLVDAYRSYSKPVFFSEFGCNKVLPRQ
FQEIGYLFSEEMYSVFCGGLVYEFSQEDNNYGLVEYQEDDSVQLLADFEKLKSHYQNI
EFPSMKTLKETVQMEETPSCAEDYENLKIESKIAKNLGSSLIKKGVKVEKGKYIDIHE
DQLSTNVTILDKHGDRWNGPKKIEIRQSLTLADLEGEEQEDADEDKDDLKRKHRNSAS
ISGPLLPLGLCLLFFTFSLFF"
rep_origin 72683..72753
/note="ARS1507; Autonomously Replicating Sequence"
/db_xref="SGD:S000118484"
gene <73031..>73357
/locus_tag="YOL131W"
/db_xref="GeneID:853989"
mRNA <73031..>73357
/locus_tag="YOL131W"
/product="uncharacterized protein"
/transcript_id="NM_001183385.1"
/db_xref="GeneID:853989"
CDS 73031..73357
/locus_tag="YOL131W"
/note="hypothetical protein; YOL131W has a paralog, STB1,
that arose from the whole genome duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014510.1"
/db_xref="GeneID:853989"
/db_xref="SGD:S000005491"
/translation="MNTNKIAQDEVQDKVLQRAELAHSVWNLRFNLSKVAKRIRMETK
VFPEIKINDAQSQLERSRCRIFSPDLEEEHVPLIQGFKCLDSPPPVPPSSSQGEDEEN
TVDSQY"
gene <74400..>76979
/gene="ALR1"
/locus_tag="YOL130W"
/gene_synonym="SWC3"
/db_xref="GeneID:853990"
mRNA <74400..>76979
/gene="ALR1"
/locus_tag="YOL130W"
/gene_synonym="SWC3"
/product="Mg(2+) transporter ALR1"
/transcript_id="NM_001183384.1"
/db_xref="GeneID:853990"
CDS 74400..76979
/gene="ALR1"
/locus_tag="YOL130W"
/gene_synonym="SWC3"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:21738593]"
/experiment="EXISTENCE:direct assay:GO:0015693 magnesium
ion transport [PMID:12167543]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0005886 plasma
membrane [PMID:9430719]"
/experiment="EXISTENCE:mutant phenotype:GO:0006812
monoatomic cation transport [PMID:9430719]"
/experiment="EXISTENCE:mutant phenotype:GO:0022857
transmembrane transporter activity [PMID:9430719]"
/note="Plasma membrane Mg(2+) transporter; expression and
turnover are regulated by Mg(2+) concentration;
overexpression confers increased tolerance to Al(3+) and
Ga(3+) ions; magnesium transport defect of the null mutant
is functionally complemented by either of the human genes
MAGT1 and TUSC3 that are not orthologous to ALR1"
/codon_start=1
/product="Mg(2+) transporter ALR1"
/protein_id="NP_014511.1"
/db_xref="GeneID:853990"
/db_xref="SGD:S000005490"
/translation="MSSSSSSSESSPNLSRSNSLANTMVSMKTEDHTGLYDHRQHPDS
LPVRHQPPTLKNKEIAKSTKPSIPKEQKSATRYNSHVDVGSVPSRGRMDFEDEGQGMD
ETVAHHQLRASAILTSNARPSRLAHSMPHQRQLYVESNIHTPPKDVGVKRDYTMSSST
ASSGNKSKLSASSSASPITKVRKSSLVSPVLEIPHESKSDTHSKLAKPKKRTYSTTSA
HSSINPAVLLTKSTSQKSDADDDTLERKPVRMNTRASFDSDVSQASRDSQETEEDVCF
PMPPQLHTRVNGIDFDELEEYAQFANAEKSQFLASLQVPNEQKYSNVSQDIGFTSSTS
TSGSSAALKYTPRVSQTGEKSESTNETEIHEKKEDEHEKIKPSLHPGISFGKNKVEGE
ENENIPSNDPAYCSYQGTDFQIPNRFSFFCSESDETVHASDIPSLVSEGQTFYELFRG
GEPTWWLDCSCPTDDEMRCIAKAFGIHPLTAEDIRMQETREKVELFKSYYFVCFHTFE
NDKESEDFLEPINVYIVVCRSGVLTFHFGPISHCANVRRRVRQLRDYVNVNSDWLCYA
LIDDITDSFAPVIQSIEYEADAIEDSVFMARDMDFAAMLQRIGESRRKTMTLMRLLSG
KADVIKMFAKRCQDEANGIGPALTSQINIANLQARQDNASHIKNNSSTTVPNNYAPTT
SQPRGDIALYLGDIQDHLLTMFQNLLAYEKIFSRSHTNYLAQLQVESFNSNNKVTEML
GKVTMIGTMLVPLNVITGLFGMNVKVPGENSSIAWWFGILGVLLLLAVLGWFLASYWI
KRIDPPATLNEAAESGAKSVISSFLPKRNKRFNDRSKNINVRAGPSNKSVASLPSRYS
RYD"
gene <77560..>78114
/gene="VPS68"
/locus_tag="YOL129W"
/db_xref="GeneID:853991"
mRNA <77560..>78114
/gene="VPS68"
/locus_tag="YOL129W"
/product="Vps68p"
/transcript_id="NM_001183383.1"
/db_xref="GeneID:853991"
CDS 77560..78114
/gene="VPS68"
/locus_tag="YOL129W"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:23708375|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:39271159]"
/experiment="EXISTENCE:direct assay:GO:0034424 Vps55/Vps68
complex [PMID:18216282]"
/experiment="EXISTENCE:mutant phenotype:GO:0006623 protein
targeting to vacuole [PMID:12134085]"
/experiment="EXISTENCE:mutant phenotype:GO:0032511 late
endosome to vacuole transport via multivesicular body
sorting pathway [PMID:18216282]"
/experiment="EXISTENCE:physical interaction:GO:0034424
Vps55/Vps68 complex [PMID:18216282]"
/note="Vacuolar membrane hypothetical protein; involved in
vacuolar protein sorting; also detected in the
mitochondria"
/codon_start=1
/product="Vps68p"
/protein_id="NP_014512.1"
/db_xref="GeneID:853991"
/db_xref="SGD:S000005489"
/translation="MEADDHVSLFRFPFKIPTFRGIRKGGVYLSGALYALGFWIFLDA
VLYSRYSNASDVHVTFIDWIPFLCSTLGTLIVNSIEKNRLLQGALSSDGGAFGSGVGD
LDSSMAWQARTVLFFGFALLAGGLSGSIVVLIIKFLVKDYNTYPTLGMGVNNVLGNVC
ILLSCVVLWIAQNVEDEYSYSLTL"
gene complement(<78352..>79479)
/gene="YGK3"
/locus_tag="YOL128C"
/db_xref="GeneID:853992"
mRNA complement(<78352..>79479)
/gene="YGK3"
/locus_tag="YOL128C"
/product="serine/threonine protein kinase YGK3"
/transcript_id="NM_001183382.1"
/db_xref="GeneID:853992"
CDS complement(78352..79479)
/gene="YGK3"
/locus_tag="YOL128C"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:mutant phenotype:GO:0004674 protein
serine/threonine kinase activity [PMID:10958669]"
/experiment="EXISTENCE:mutant phenotype:GO:0006508
proteolysis [PMID:10958669]"
/note="Protein kinase related to mammalian GSK-3 glycogen
synthase kinases; GSK-3 homologs (Mck1p, Rim11p, Mrk1p,
Ygk3p) are involved in control of Msn2p-dependent
transcription of stress responsive genes and in protein
degradation; YGK3 has a paralog, MCK1, that arose from the
whole genome duplication"
/codon_start=1
/product="serine/threonine protein kinase YGK3"
/protein_id="NP_014513.1"
/db_xref="GeneID:853992"
/db_xref="SGD:S000005488"
/translation="MLKVNNVFGSNPNRMTKLEDEHYFIDDIVSIKNRQKSKMYVREG
KRIGHGSFGTVTQSILSSNSIEWLGPYAIKRVVKSPKVQSLELEILQNIRHPNLVTLE
FFFESHCTTKDGGHLYQKNFVMEYIPQTLSSEIHEYFDNGSKMPTKHIKLYTFQILRA
LLTLHSMSICHGDLKPSNILIIPSSGIAKVCDFGSAQRLDDNTELKTYFCSRFYRAPE
LLLNSKDYTTQIDIWSLGCIIGEMIKGQPLFKGDSANSQLEEIAKLLGRFPKSSIKNS
QELQDSLNDQKFKKFMHWFPSIEFFDVEFLLKVLTYDATERCDARQLMAHEFFDALRN
ETYFLPRGSSMPVHLPDLFNFSASEKRALGEYYNLIVPSLD"
gene <80348..>81190
/gene="RPL25"
/locus_tag="YOL127W"
/db_xref="GeneID:853993"
mRNA join(<80348..80360,80775..>81190)
/gene="RPL25"
/locus_tag="YOL127W"
/product="60S ribosomal protein uL23 RPL25"
/transcript_id="NM_001183381.1"
/db_xref="GeneID:853993"
CDS join(80348..80360,80775..81190)
/gene="RPL25"
/locus_tag="YOL127W"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:11983894]"
/experiment="EXISTENCE:curator inference:GO:0003735
structural constituent of ribosome [PMID:11983894]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:8028007]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:11983894]"
/experiment="EXISTENCE:direct assay:GO:0030687
preribosome, large subunit precursor [PMID:23268442]"
/experiment="EXISTENCE:mutant phenotype:GO:0000027
ribosomal large subunit assembly [PMID:8028007]"
/note="Ribosomal 60S subunit protein L25; primary
rRNA-binding ribosomal protein component of large
ribosomal subunit; binds to 25S rRNA via a conserved
C-terminal motif; homologous to mammalian ribosomal
protein L23A and bacterial L23"
/codon_start=1
/product="60S ribosomal protein uL23 RPL25"
/protein_id="NP_014514.1"
/db_xref="GeneID:853993"
/db_xref="SGD:S000005487"
/translation="MAPSAKATAAKKAVVKGTNGKKALKVRTSATFRLPKTLKLARAP
KYASKAVPHYNRLDSYKVIEQPITSETAMKKVEDGNILVFQVSMKANKYQIKKAVKEL
YEVDVLKVNTLVRPNGTKKAYVRLTADYDALDIANRIGYI"
gene complement(<81787..>82920)
/gene="MDH2"
/locus_tag="YOL126C"
/db_xref="GeneID:853994"
mRNA complement(<81787..>82920)
/gene="MDH2"
/locus_tag="YOL126C"
/product="malate dehydrogenase MDH2"
/transcript_id="NM_001183380.1"
/db_xref="GeneID:853994"
CDS complement(81787..82920)
/gene="MDH2"
/locus_tag="YOL126C"
/EC_number="1.1.1.37"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:1986231]"
/experiment="EXISTENCE:direct assay:GO:0030060 L-malate
dehydrogenase (NAD+) activity [PMID:1986231]"
/experiment="EXISTENCE:direct assay:GO:0034399 nuclear
periphery [PMID:25817432]"
/experiment="EXISTENCE:mutant phenotype:GO:0006094
gluconeogenesis [PMID:12730240]"
/experiment="EXISTENCE:mutant phenotype:GO:0016558 protein
import into peroxisome matrix [PMID:19118449]"
/experiment="EXISTENCE:mutant phenotype:GO:0030060
L-malate dehydrogenase (NAD+) activity [PMID:1986231]"
/note="Cytoplasmic malate dehydrogenase; one of three
isozymes that catalyze interconversion of malate and
oxaloacetate; involved in the glyoxylate cycle and
gluconeogenesis during growth on two-carbon compounds;
interacts with Pck1p and Fbp1; mutation in human homolog
MDH2 causes early-onset severe encephalopathy"
/codon_start=1
/product="malate dehydrogenase MDH2"
/protein_id="NP_014515.2"
/db_xref="GeneID:853994"
/db_xref="SGD:S000005486"
/translation="MPHSVTPSIEQDSLKIAILGAAGGIGQSLSLLLKAQLQYQLKES
NRSVTHIHLALYDVNQEAINGVTADLSHIDTPISVSSHSPAGGIENCLHNASIVVIPA
GVPRKPGMTRDDLFNVNAGIISQLGDSIAECCDLSKVFVLVISNPVNSLVPVMVSNIL
KNHPQSRNSGIERRIMGVTKLDIVRASTFLREINIESGLTPRVNSMPDVPVIGGHSGE
TIIPLFSQSNFLSRLNEDQLKYLIHRVQYGGDEVVKAKNGKGSATLSMAHAGYKCVVQ
FVSLLLGNIEQIHGTYYVPLKDANNFPIAPGADQLLPLVDGADYFAIPLTITTKGVSY
VDYDIVNRMNDMERNQMLPICVSQLKKNIDKGLEFVASRSASS"
gene <83834..>85264
/gene="TRM13"
/locus_tag="YOL125W"
/db_xref="GeneID:853995"
mRNA <83834..>85264
/gene="TRM13"
/locus_tag="YOL125W"
/product="tRNA:m4X modification enzyme"
/transcript_id="NM_001183379.2"
/db_xref="GeneID:853995"
CDS 83834..85264
/gene="TRM13"
/locus_tag="YOL125W"
/EC_number="2.1.1.225"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0030488 tRNA
methylation [PMID:17242307]"
/experiment="EXISTENCE:direct assay:GO:0106050 tRNA
2'-O-methyltransferase activity [PMID:17242307]"
/experiment="EXISTENCE:mutant phenotype:GO:0002128 tRNA
nucleoside ribose methylation [PMID:32053677]"
/experiment="EXISTENCE:mutant phenotype:GO:0030488 tRNA
methylation [PMID:17242307]"
/experiment="EXISTENCE:mutant phenotype:GO:0106050 tRNA
2'-O-methyltransferase activity [PMID:17242307]"
/note="2'-O-methyltransferase; responsible for
modification of tRNA at position 4; C-terminal domain has
similarity to Rossmann-fold (RFM) superfamily of RNA
methyltransferases"
/codon_start=1
/product="tRNA:m4X modification enzyme"
/protein_id="NP_014516.1"
/db_xref="GeneID:853995"
/db_xref="SGD:S000005485"
/translation="MLQDNNGPAVKRAKPSERLQCEYFMEKKKRRCGMTRSSQNLYCS
EHLNLMKKAANSQVHNKNGSEAEKERERVPCPLDPNHTVWADQLKKHLKKCNKTKLSH
LNDDKPYYEPGYNGENGLLSSSVKIDITAEHLVQSIELLYKVFEGESMDELPLRQLNN
KLMSLKRFPQLPSNTKHAVQQSSLIENLVDAGAFERPESLNFIEFGCGRAEFSRYVSL
YLLTQLTSLPAEHSGSNSNEFVLIDRATNRMKFDKKIKDDFSEIKSNSPSKPISCPSI
KRIKIDIRDLKMDPILKSTPGDDIQYVCISKHLCGVATDLTLRCIGNSSILHGDDNNG
CNPKLKAICIAMCCRHVCDYGDYVNRSYVTSLVEKYRAHGSILTYETFFRVLTKLCSW
GTCGRKPGTAITDIVNVVESFEGAEPYTITIKERENIGLMARRVIDEGRLVYVKEKFT
EFNAELIRYVESDVSLENVAMLVYKK"
rep_origin 85270..85361
/note="ARS1508; Autonomously Replicating Sequence"
/db_xref="SGD:S000118485"
gene complement(<85456..>86757)
/gene="TRM11"
/locus_tag="YOL124C"
/db_xref="GeneID:853996"
mRNA complement(<85456..>86757)
/gene="TRM11"
/locus_tag="YOL124C"
/product="tRNA (guanine-N2-)-methyltransferase"
/transcript_id="NM_001183378.1"
/db_xref="GeneID:853996"
CDS complement(85456..86757)
/gene="TRM11"
/locus_tag="YOL124C"
/EC_number="2.1.1.214"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:15899842]"
/experiment="EXISTENCE:direct assay:GO:0030488 tRNA
methylation [PMID:15899842]"
/experiment="EXISTENCE:direct assay:GO:0160102 tRNA
(guanine(10)-N2)-methyltransferase activity
[PMID:15899842]"
/experiment="EXISTENCE:mutant phenotype:GO:0030488 tRNA
methylation [PMID:15899842]"
/experiment="EXISTENCE:mutant phenotype:GO:0160102 tRNA
(guanine(10)-N2)-methyltransferase activity
[PMID:15899842]"
/experiment="EXISTENCE:physical interaction:GO:0043528
tRNA (m2G10) methyltransferase complex [PMID:15899842]"
/note="Catalytic subunit of adoMet-dependent tRNA
methyltransferase complex; required for the methylation of
the guanosine nucleotide at position 10 (m2G10) in tRNAs;
involved in the methylation of guanosine and uridine
ribonucleosides in mRNA; contains a THUMP domain and a
methyltransferase domain; methyltransferase complex also
contains Trm112p"
/codon_start=1
/product="tRNA (guanine-N2-)-methyltransferase"
/protein_id="NP_014517.1"
/db_xref="GeneID:853996"
/db_xref="SGD:S000005484"
/translation="MKKYLLYMVQVHLNFRRAELESLADLYNLSIDFSQYDANSPFFI
VELENDQQAKDWIKRSILTRGIYEYWGQGTTLDELHKDIQRQSNFEQDLQLKFKHSTF
KFEFECYKGNSKAKRVEQIETFRYLGFEGKIDMKHPQEVFTVIEEYTPISENVGGKTP
TRIYFGRQVQMSNRSAMEKYDLKKRPYKGTTSFEAELSLVSANIAQVKPGTIMYDPFA
GTGSFLVAGGHFGSLVIGSDIDGRMIRGKGAQVNISANFKKYGESSQFLDVLTMDFTN
NALRNNLVIDTILCDPPYGIRESIKVLGAKDPERFLGKEDMEIDGEKAYLRRDYIPTK
KPYALDSLLDDLLQYSSERLPIGGRLAFWMPTANDANIETIVPMHENLELKYNCVQEF
NKWSRRLLVYINRGSTFNGSSNHGIKRSKDNFRERYFNNFN"
gene <87844..>89448
/gene="HRP1"
/locus_tag="YOL123W"
/gene_synonym="NAB4; NAB5"
/db_xref="GeneID:853997"
mRNA <87844..>89448
/gene="HRP1"
/locus_tag="YOL123W"
/gene_synonym="NAB4; NAB5"
/product="Hrp1p"
/transcript_id="NM_001183377.1"
/db_xref="GeneID:853997"
CDS 87844..89448
/gene="HRP1"
/locus_tag="YOL123W"
/gene_synonym="NAB4; NAB5"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:9334319]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640|PMID:17684230|PMID:15703440]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11914276|PMID:15572678|PMID:9334319]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:9334319]"
/experiment="EXISTENCE:direct assay:GO:0006397 mRNA
processing [PMID:11344258]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405|PMID:18981231]"
/experiment="EXISTENCE:mutant phenotype:GO:0072423
response to DNA damage checkpoint signaling
[PMID:24603480]"
/experiment="EXISTENCE:physical interaction:GO:0005849
mRNA cleavage factor complex [PMID:11344258]"
/note="Subunit of cleavage factor I and mRNA guard
protein; cleavage factor I is a five-subunit complex
required for the cleavage and polyadenylation of pre-mRNA
3' ends; RRM-containing heteronuclear RNA binding protein
and hnRNPA/B family member that binds to poly (A) signal
sequences; required for genome stability; plays a role in
copper homeostasis"
/codon_start=1
/product="Hrp1p"
/protein_id="NP_014518.1"
/db_xref="GeneID:853997"
/db_xref="SGD:S000005483"
/translation="MSSDEEDFNDIYGDDKPTTTEEVKKEEEQNKAGSGTSQLDQLAA
LQALSSSLNKLNNPNSNNSSSNNSNQDTSSSKQDGTANDKEGSNEDTKNEKKQESATS
ANANANASSAGPSGLPWEQLQQTMSQFQQPSSQSPPQQQVTQTKEERSKADLSKESCK
MFIGGLNWDTTEDNLREYFGKYGTVTDLKIMKDPATGRSRGFGFLSFEKPSSVDEVVK
TQHILDGKVIDPKRAIPRDEQDKTGKIFVGGIGPDVRPKEFEEFFSQWGTIIDAQLML
DKDTGQSRGFGFVTYDSADAVDRVCQNKFIDFKDRKIEIKRAEPRHMQQKSSNNGGNN
GGNNMNRRGGNFGNQGDFNQMYQNPMMGGYNPMMNPQAMTDYYQKMQEYYQQMQKQTG
MDYTQMYQQQMQQMAMMMPGFAMPPNAMTLNQPQQDSNATQGSPAPSDSDNNKSNDVQ
TIGNTSNTDSGSPPLNLPNGPKGPSQYNDDHNSGYGYNRDRGDRDRNDRDRDYNHRSG
GNHRRNGRGGRGGYNRRNNGYHPYNR"
gene complement(<89692..>91419)
/gene="SMF1"
/locus_tag="YOL122C"
/gene_synonym="SBS1"
/db_xref="GeneID:854027"
mRNA complement(<89692..>91419)
/gene="SMF1"
/locus_tag="YOL122C"
/gene_synonym="SBS1"
/product="divalent metal ion transporter SMF1"
/transcript_id="NM_001183376.1"
/db_xref="GeneID:854027"
CDS complement(89692..91419)
/gene="SMF1"
/locus_tag="YOL122C"
/gene_synonym="SBS1"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:8643535]"
/experiment="EXISTENCE:direct assay:GO:0015295
solute:proton symporter activity [PMID:10574989]"
/experiment="EXISTENCE:direct assay:GO:0022857
transmembrane transporter activity [PMID:10574989]"
/experiment="EXISTENCE:genetic interaction:GO:0006825
copper ion transport [PMID:9115231]"
/experiment="EXISTENCE:genetic interaction:GO:0006826 iron
ion transport [PMID:10930410]"
/experiment="EXISTENCE:genetic interaction:GO:0006878
intracellular copper ion homeostasis [PMID:9115231]"
/experiment="EXISTENCE:genetic interaction:GO:0015691
cadmium ion transport [PMID:9115231]"
/experiment="EXISTENCE:mutant phenotype:GO:0006828
manganese ion transport [PMID:8643535]"
/experiment="EXISTENCE:mutant phenotype:GO:0030026
intracellular manganese ion homeostasis [PMID:8643535]"
/note="Divalent metal ion transporter; broad specificity
for di-valent and tri-valent metals; post-translationally
regulated by levels of metal ions; member of the Nramp
family of metal transport proteins"
/codon_start=1
/product="divalent metal ion transporter SMF1"
/protein_id="NP_014519.1"
/db_xref="GeneID:854027"
/db_xref="SGD:S000005482"
/translation="MVNVGPSHAAVAVDASEARKRNISEEVFELRDKKDSTVVIEGEA
PVRTFTSSSSNHEREDTYVSKRQVMRDIFAKYLKFIGPGLMVSVAYIDPGNYSTAVDA
GASNQFSLLCIILLSNFIAIFLQCLCIKLGSVTGLDLSRACREYLPRWLNWTLYFFAE
CAVIATDIAEVIGTAIALNILIKVPLPAGVAITVVDVFLIMFTYKPGASSIRFIRIFE
CFVAVLVVGVCICFAIELAYIPKSTSVKQVFRGFVPSAQMFDHNGIYTAISILGATVM
PHSLFLGSALVQPRLLDYDVKHGNYTVSEEQDKVKKSKSTEEIMEEKYFNYRPTNAAI
KYCMKYSMVELSITLFTLALFVNCAILVVAGSTLYNSPEADGADLFTIHELLSRNLAP
AAGTIFMLALLLSGQSAGVVCTMSGQIVSEGHINWKLQPWQRRLATRCISIIPCLVIS
ICIGREALSKALNASQVVLSIVLPFLVAPLIFFTCKKSIMKTEITVDHTEEDSHNHQN
NNDRSAGSVIEQDGSSGMEIENGKDVKIVYMANNWIITVIAIIVWLFLSLLNVYAIVQ
LGMSHGDIS"
gene complement(<92026..>92850)
/gene="RPS19A"
/locus_tag="YOL121C"
/db_xref="GeneID:854028"
mRNA complement(join(<92026..92440,92831..>92850))
/gene="RPS19A"
/locus_tag="YOL121C"
/product="40S ribosomal protein eS19 RPS19A"
/transcript_id="NM_001183375.1"
/db_xref="GeneID:854028"
CDS complement(join(92026..92440,92831..92850))
/gene="RPS19A"
/locus_tag="YOL121C"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:6814480]"
/experiment="EXISTENCE:direct assay:GO:0022627 cytosolic
small ribosomal subunit [PMID:6814480]"
/experiment="EXISTENCE:genetic interaction:GO:0000054
ribosomal subunit export from nucleus
[PMID:16246728|PMID:16159874]"
/experiment="EXISTENCE:genetic interaction:GO:0042274
ribosomal small subunit biogenesis [PMID:16159874]"
/experiment="EXISTENCE:mutant phenotype:GO:0000054
ribosomal subunit export from nucleus [PMID:16159874]"
/experiment="EXISTENCE:mutant phenotype:GO:0042274
ribosomal small subunit biogenesis [PMID:16159874]"
/note="Protein component of the small (40S) ribosomal
subunit; required for assembly and maturation of pre-40 S
particles; homologous to mammalian ribosomal protein S19,
no bacterial homolog; mutations in human RPS19 are
associated with Diamond Blackfan anemia; RPS19A has a
paralog, RPS19B, that arose from the whole genome
duplication"
/codon_start=1
/product="40S ribosomal protein eS19 RPS19A"
/protein_id="NP_014520.1"
/db_xref="GeneID:854028"
/db_xref="SGD:S000005481"
/translation="MPGVSVRDVAAQDFINAYASFLQRQGKLEVPGYVDIVKTSSGNE
MPPQDAEGWFYKRAASVARHIYMRKQVGVGKLNKLYGGAKSRGVRPYKHIDASGSINR
KVLQALEKIGIVEISPKGGRRISENGQRDLDRIAAQTLEEDE"
gene complement(<93395..>94402)
/gene="RPL18A"
/locus_tag="YOL120C"
/gene_synonym="RP28A"
/db_xref="GeneID:854029"
mRNA complement(join(<93395..93843,94291..>94402))
/gene="RPL18A"
/locus_tag="YOL120C"
/gene_synonym="RP28A"
/product="60S ribosomal protein eL18 RPL18A"
/transcript_id="NM_001183374.1"
/db_xref="GeneID:854029"
CDS complement(join(93395..93843,94291..94402))
/gene="RPL18A"
/locus_tag="YOL120C"
/gene_synonym="RP28A"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:11983894]"
/experiment="EXISTENCE:curator inference:GO:0003735
structural constituent of ribosome [PMID:11983894]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:11983894]"
/note="Ribosomal 60S subunit protein L18A; intron of
RPL18A pre-mRNA forms stem-loop structures that are a
target for Rnt1p cleavage leading to degradation;
homologous to mammalian ribosomal protein L18, no
bacterial homolog; RPL18A has a paralog, RPL18B, that
arose from the whole genome duplication"
/codon_start=1
/product="60S ribosomal protein eL18 RPL18A"
/protein_id="NP_014521.1"
/db_xref="GeneID:854029"
/db_xref="SGD:S000005480"
/translation="MGIDHTSKQHKRSGHRTAPKSDNVYLKLLVKLYTFLARRTDAPF
NKVVLKALFLSKINRPPVSVSRIARALKQEGAANKTVVVVGTVTDDARIFEFPKTTVA
ALRFTAGARAKIVKAGGECITLDQLAVRAPKGQNTLILRGPRNSREAVRHFGMGPHKG
KAPRILSTGRKFERARGRRRSKGFKV"
gene complement(<94856..>96361)
/gene="MCH4"
/locus_tag="YOL119C"
/db_xref="GeneID:854030"
mRNA complement(<94856..>96361)
/gene="MCH4"
/locus_tag="YOL119C"
/product="Mch4p"
/transcript_id="NM_001183373.1"
/db_xref="GeneID:854030"
CDS complement(94856..96361)
/gene="MCH4"
/locus_tag="YOL119C"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:11536335]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="Protein with similarity to mammalian
monocarboxylate permeases; monocarboxylate permeases are
involved in transport of monocarboxylic acids across the
plasma membrane but mutant is not deficient in
monocarboxylate transport"
/codon_start=1
/product="Mch4p"
/protein_id="NP_014522.1"
/db_xref="GeneID:854030"
/db_xref="SGD:S000005479"
/translation="MLNIPIIANSKRFLFSKDHEAQSTRDHDVELETREGPSSGYNPN
FNAADAILKKNSDQVDLDVNKLTNVTSRVLNTPEASLIYDDDREFPDGGLKAWLVVFG
AFMGLVPVFGLINSLGAIESYISKHQLANISSSTISWIFSLYLAISFLSCILSGGYFD
RNGSIGLMCTGTVIYAGGLFALANCKSVWQFILAFSVCSGLGTGILMTPLIGTVATWF
LKRRGIATSISTMGGSIGGIVFPIMLRKLYKEVGFQWAIRILSFICLTCLICASVLAR
ERTKPVVQPFKSKAEVAKWYISSVFNWRYFLEGKFLFVAIGASFAESSLTSCATYLAS
YSMTRGNTENVAYTMITASNAVGILGRYIPGYFADKFIGRFNVEIITISMAALFNFVM
WLPFGGNTKVLWAYVCLWGFSTGSILSLTPVCIGQISKTTDFGKRYATVYLLQALVTI
PVLPIGGTLIGKGTVANYNHFIIFNSALMAAGAACYIISRHICVGAKLCKF"
gene complement(<96608..>96916)
/locus_tag="YOL118C"
/db_xref="GeneID:854031"
mRNA complement(<96608..>96916)
/locus_tag="YOL118C"
/product="uncharacterized protein"
/transcript_id="NM_001348874.1"
/db_xref="GeneID:854031"
CDS complement(96608..96916)
/locus_tag="YOL118C"
/note="hypothetical protein; conserved across S.
cerevisiae strains"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335812.1"
/db_xref="GeneID:854031"
/db_xref="SGD:S000005478"
/translation="MSFRKKKLKPPAGSQFIINDSIMSYIDRTKTLIRMIGCKNQYIK
ARMKDKTFFYTKQFRTAKNKFFFHLYHWEATHINVDHYICTCHPIFWGSIGQKLRRSA
"
gene <97551..>99488
/gene="RRI2"
/locus_tag="YOL117W"
/gene_synonym="CSN10"
/db_xref="GeneID:854032"
mRNA <97551..>99488
/gene="RRI2"
/locus_tag="YOL117W"
/gene_synonym="CSN10"
/product="Rri2p"
/transcript_id="NM_001183371.1"
/db_xref="GeneID:854032"
CDS 97551..99488
/gene="RRI2"
/locus_tag="YOL117W"
/gene_synonym="CSN10"
/experiment="EXISTENCE:direct assay:GO:0008180 COP9
signalosome [PMID:12446563]"
/experiment="EXISTENCE:genetic interaction:GO:0000747
conjugation with cellular fusion [PMID:12446563]"
/experiment="EXISTENCE:genetic interaction:GO:0071444
cellular response to pheromone [PMID:12446563]"
/experiment="EXISTENCE:mutant phenotype:GO:0000338 protein
deneddylation [PMID:12446563|PMID:12186635]"
/note="Subunit of the COP9 signalosome (CSN) complex; this
complex cleaves the ubiquitin-like protein Nedd8 from SCF
ubiquitin ligases; plays a role in the mating pheromone
response"
/codon_start=1
/product="Rri2p"
/protein_id="NP_014524.1"
/db_xref="GeneID:854032"
/db_xref="SGD:S000005477"
/translation="MSDEDNNYDDFMLSDDEGMESIEMEEETDDEDKQNIEINEDNSQ
DDQDRGAARHKQHEQGTFEKHDRVEDICERIFEQGQALKEDERYKEARDLFLKIYYKE
EFSSDESIERLMTWKFKSLIEILRLRALQLYFQKNGAQDLVLQILEDTATMSVFLQRI
DFQIDGNIFELLSDTFEVLAPKWERVFLFDIEKVDRENMICKIDFQKNFMDQFQWILR
KPGKDCKLQNLQRIIRKKIFIAVVWYQRLTMGNVFTPEISSQIEILVKDNECSSFEEN
NDLESVSMLLQYYILEYMNTARINNRRLFKKCIDFFEMLISKSLTFSQESGLMVILYT
SKIVFILDSDSENDLSFALMRYYDRKEELKNMFLYILKHLEEMGKLRERDITSLFHKF
ILSGFIFTSMILEAISTDKINPFGFEQVKIALGSPIVNVLEDVYRCFAQLELRQLNAS
ISLIPELSVVLSGIIQDIYYLAQTLKLWRKIARLYSCISISDIISMLQISDDNEMTRD
DLLTILMRSIMKNRSVVYFKLDLTSDLVYFGDENKVMLPRCSKEEFRLMISPKDEETT
EKARLIDFEYVNDVAIYNNPTRIRTKSSKEFFNTLRKSRETVKLPRVSNQSNEDTFLP
SYMKFSNKYLELCKLASNNLE"
gene <99809..>100957
/gene="MSN1"
/locus_tag="YOL116W"
/gene_synonym="FUP1; HRB382; MSS10; PHD2"
/db_xref="GeneID:854033"
mRNA <99809..>100957
/gene="MSN1"
/locus_tag="YOL116W"
/gene_synonym="FUP1; HRB382; MSS10; PHD2"
/product="Msn1p"
/transcript_id="NM_001183370.1"
/db_xref="GeneID:854033"
CDS 99809..100957
/gene="MSN1"
/locus_tag="YOL116W"
/gene_synonym="FUP1; HRB382; MSS10; PHD2"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:2263457|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:20802496]"
/experiment="EXISTENCE:genetic interaction:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:10409737]"
/experiment="EXISTENCE:genetic interaction:GO:0071475
cellular hyperosmotic salinity response [PMID:10409737]"
/experiment="EXISTENCE:mutant phenotype:GO:0034605
cellular response to heat [PMID:10409737]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:8662191|PMID:8710886|PMID:10409737|PMID:9987114|PMID
:15773992]"
/experiment="EXISTENCE:mutant phenotype:GO:0071475
cellular hyperosmotic salinity response [PMID:10409737]"
/experiment="EXISTENCE:mutant phenotype:GO:0097238
cellular response to methylglyoxal [PMID:15773992]"
/experiment="EXISTENCE:mutant phenotype:GO:2000219
positive regulation of invasive growth in response to
glucose limitation [PMID:8710886]"
/experiment="EXISTENCE:mutant phenotype:GO:2000222
positive regulation of pseudohyphal growth [PMID:8710886]"
/experiment="EXISTENCE:mutant phenotype:GO:2000883
positive regulation of starch catabolic process
[PMID:9987114|PMID:8662191]"
/note="Transcriptional activator; involved in regulation
of invertase and glucoamylase expression, invasive growth
and pseudohyphal differentiation, iron uptake, chromium
accumulation, and response to osmotic stress; localizes to
the nucleus; relative distribution to the nucleus
increases upon DNA replication stress"
/codon_start=1
/product="Msn1p"
/protein_id="NP_014525.1"
/db_xref="GeneID:854033"
/db_xref="SGD:S000005476"
/translation="MASNQHIGASNLNENEAILTNRVAELERRMSMFEGIFHALSNRL
DLHFKKYDVVVNSQQQQINELTAFLSTLLNDQQRHAEILSEKLSGTLHGVSATSISLS
QTLDPQGFTDGTTAPGAPRNYTSVPMNNDQTAHPQNEGAVSNETLFEDILNGNSQEND
KSQQQTNSSNSISQENNSTNPSVDTRFNKPQNYNSNLVPSLEEYSANPPNNDGGQSQG
LYISSNSSQSRQSPNLQKVSPNHENAVESNAQESVPTFEEEQYETKTGLKRKRIVCTR
PFEFIKSPHSVMEVWKEYTEGVNGQPSIRKMEALYQTAWRRDPAVNKRYSRRKVLWKA
IQTGLNRGYSLNYVVEILENSRYVNDKQKVKQPIGWLCHSSHIPETLK"
gene <101475..>103229
/gene="PAP2"
/locus_tag="YOL115W"
/gene_synonym="TRF4"
/db_xref="GeneID:854034"
mRNA <101475..>103229
/gene="PAP2"
/locus_tag="YOL115W"
/gene_synonym="TRF4"
/product="non-canonical poly(A) polymerase PAP2"
/transcript_id="NM_001183369.1"
/db_xref="GeneID:854034"
CDS 101475..103229
/gene="PAP2"
/locus_tag="YOL115W"
/gene_synonym="TRF4"
/EC_number="2.7.7.19"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10066793|PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:16541108]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0031499 TRAMP
complex [PMID:15935759|PMID:15935758|PMID:15828860]"
/experiment="EXISTENCE:direct assay:GO:0034458 3'-5' RNA
helicase activity [PMID:22532666]"
/experiment="EXISTENCE:direct assay:GO:0051575
5'-deoxyribose-5-phosphate lyase activity [PMID:17983848]"
/experiment="EXISTENCE:direct assay:GO:0071038
TRAMP-dependent tRNA surveillance pathway
[PMID:17643380|PMID:15935758|PMID:15828860]"
/experiment="EXISTENCE:direct assay:GO:1990817 poly(A) RNA
polymerase activity
[PMID:16374505|PMID:15935758|PMID:16260630|PMID:15935759|P
MID:12062100]"
/experiment="EXISTENCE:genetic interaction:GO:0006284
base-excision repair [PMID:17983848]"
/experiment="EXISTENCE:genetic interaction:GO:0034475 U4
snRNA 3'-end processing [PMID:16373491]"
/experiment="EXISTENCE:genetic interaction:GO:0071031
nuclear mRNA surveillance of mRNA 3'-end processing
[PMID:17410208]"
/experiment="EXISTENCE:genetic interaction:GO:0071035
nuclear polyadenylation-dependent rRNA catabolic process
[PMID:15935758|PMID:16541108]"
/experiment="EXISTENCE:genetic interaction:GO:0071036
nuclear polyadenylation-dependent snoRNA catabolic process
[PMID:16373491|PMID:15935758]"
/experiment="EXISTENCE:genetic interaction:GO:0071038
TRAMP-dependent tRNA surveillance pathway [PMID:15145828]"
/experiment="EXISTENCE:genetic interaction:GO:0071039
nuclear polyadenylation-dependent CUT catabolic process
[PMID:15935759]"
/experiment="EXISTENCE:genetic interaction:GO:0071042
nuclear polyadenylation-dependent mRNA catabolic process
[PMID:16373491]"
/experiment="EXISTENCE:genetic interaction:GO:0071044
histone mRNA catabolic process [PMID:17179095]"
/experiment="EXISTENCE:genetic interaction:GO:0071051
poly(A)-dependent snoRNA 3'-end processing
[PMID:16373491|PMID:18951092]"
/experiment="EXISTENCE:genetic interaction:GO:1990817
poly(A) RNA polymerase activity [PMID:16374505]"
/experiment="EXISTENCE:mutant phenotype:GO:0006284
base-excision repair [PMID:17983848]"
/experiment="EXISTENCE:mutant phenotype:GO:0006400 tRNA
modification [PMID:22319136]"
/experiment="EXISTENCE:mutant phenotype:GO:0034475 U4
snRNA 3'-end processing [PMID:16373491]"
/experiment="EXISTENCE:mutant phenotype:GO:0042138 meiotic
DNA double-strand break formation [PMID:25210768]"
/experiment="EXISTENCE:mutant phenotype:GO:0045910
negative regulation of DNA recombination [PMID:23762389]"
/experiment="EXISTENCE:mutant phenotype:GO:0051575
5'-deoxyribose-5-phosphate lyase activity [PMID:17983848]"
/experiment="EXISTENCE:mutant phenotype:GO:0071035 nuclear
polyadenylation-dependent rRNA catabolic process
[PMID:16431988|PMID:18007593|PMID:15935758]"
/experiment="EXISTENCE:mutant phenotype:GO:0071036 nuclear
polyadenylation-dependent snoRNA catabolic process
[PMID:15935758]"
/experiment="EXISTENCE:mutant phenotype:GO:0071037 nuclear
polyadenylation-dependent snRNA catabolic process
[PMID:16431988|PMID:15935758]"
/experiment="EXISTENCE:mutant phenotype:GO:0071038
TRAMP-dependent tRNA surveillance pathway
[PMID:16431988|PMID:18456844]"
/experiment="EXISTENCE:mutant phenotype:GO:0071039 nuclear
polyadenylation-dependent CUT catabolic process
[PMID:16973436|PMID:15935759|PMID:18591258|PMID:18007593]"
/experiment="EXISTENCE:mutant phenotype:GO:0071040 nuclear
polyadenylation-dependent antisense transcript catabolic
process [PMID:18022365]"
/experiment="EXISTENCE:mutant phenotype:GO:0071047
polyadenylation-dependent mRNA catabolic process
[PMID:19369424]"
/experiment="EXISTENCE:mutant phenotype:GO:1990817 poly(A)
RNA polymerase activity
[PMID:15935759|PMID:15828860|PMID:16260630]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:27076633]"
/note="Non-canonical poly(A) polymerase; involved in
nuclear RNA degradation as a component of TRAMP; catalyzes
polyadenylation of hypomodified tRNAs, and snoRNA and rRNA
precursors; required for mRNA surveillance and maintenance
of genome integrity, serving as a link between RNA and DNA
metabolism; overlapping but non-redundant functions with
Trf5p; relocalizes to cytosol in response to hypoxia"
/codon_start=1
/product="non-canonical poly(A) polymerase PAP2"
/protein_id="NP_014526.1"
/db_xref="GeneID:854034"
/db_xref="SGD:S000005475"
/translation="MGAKSVTASSSKKIKNRHNGKVKKSKKIKKVRKPQKSISLNDEN
EVEILPSRNEQETNKLPKDHVTADGILVLEHKSDDDEGFDVYDGHFDNPTDIPSTTEE
SKTPSLAVHGDEKDLANNDDFISLSASSEDEQAEQEEEREKQELEIKKEKQKEILNTD
YPWILNHDHSKQKEISDWLTFEIKDFVAYISPSREEIEIRNQTISTIREAVKQLWPDA
DLHVFGSYSTDLYLPGSDIDCVVTSELGGKESRNNLYSLASHLKKKNLATEVEVVAKA
RVPIIKFVEPHSGIHIDVSFERTNGIEAAKLIREWLDDTPGLRELVLIVKQFLHARRL
NNVHTGGLGGFSIICLVFSFLHMHPRIITNEIDPKDNLGVLLIEFFELYGKNFGYDDV
ALGSSDGYPVYFPKSTWSAIQPIKNPFSLAIQDPGDESNNISRGSFNIRDIKKAFAGA
FDLLTNRCFELHSATFKDRLGKSILGNVIKYRGKARDFKDERGLVLNKAIIENENYHK
KRSRIIHDEDFAEDTVTSTATATTTDDDYEITNPPAKKAKIEEKPESEPAKRNSGETY
ITVSSEDDDEDGYNPYTL"
gene complement(<103317..>103925)
/gene="PTH4"
/locus_tag="YOL114C"
/db_xref="GeneID:854035"
mRNA complement(<103317..>103925)
/gene="PTH4"
/locus_tag="YOL114C"
/product="Pth4p"
/transcript_id="NM_001183368.1"
/db_xref="GeneID:854035"
CDS complement(103317..103925)
/gene="PTH4"
/locus_tag="YOL114C"
/experiment="EXISTENCE:direct assay:GO:0004045
peptidyl-tRNA hydrolase activity [PMID:33686140]"
/experiment="EXISTENCE:direct assay:GO:0005762
mitochondrial large ribosomal subunit [PMID:25683707]"
/experiment="EXISTENCE:mutant phenotype:GO:0032543
mitochondrial translation [PMID:33686140]"
/note="Stalled-ribosome rescue factor srRF1; similar to
human peptidyl-tRNA hydrolase ICT1; associates with
mitochondrial large subunit; not an essential gene"
/codon_start=1
/product="Pth4p"
/protein_id="NP_014527.1"
/db_xref="GeneID:854035"
/db_xref="SGD:S000005474"
/translation="MTTLMGKFKLTGRSPLFVLQPMLHCKKQQFVEEAVRLISNKKIG
KKSDFVQARNWVGALNVTGLPLNQFILRYDRASGPGGQNVNKVNSKCTLTLSGLSNCA
WIPQEVRNILSSGRFRYYAKGSDSIVIQSDETRSRETNKLKCFEKLVQEIRQTCQFPN
DTTAETSKKWNKIKEKANKERLLDKKVHSDKKKNRSKIKFNY"
gene <104326..>106293
/gene="SKM1"
/locus_tag="YOL113W"
/db_xref="GeneID:854036"
mRNA <104326..>106293
/gene="SKM1"
/locus_tag="YOL113W"
/product="putative serine/threonine protein kinase SKM1"
/transcript_id="NM_001183367.1"
/db_xref="GeneID:854036"
CDS 104326..106293
/gene="SKM1"
/locus_tag="YOL113W"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:19793923]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:15023338]"
/experiment="EXISTENCE:genetic interaction:GO:0010629
negative regulation of gene expression [PMID:19793923]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:19793923]"
/experiment="EXISTENCE:mutant phenotype:GO:0000920 septum
digestion after cytokinesis [PMID:9044278]"
/experiment="EXISTENCE:mutant phenotype:GO:0005634 nucleus
[PMID:19793923]"
/experiment="EXISTENCE:mutant phenotype:GO:0010629
negative regulation of gene expression [PMID:19793923]"
/experiment="EXISTENCE:mutant phenotype:GO:0035376 sterol
import [PMID:19793923]"
/experiment="EXISTENCE:mutant phenotype:GO:2000910
negative regulation of sterol import [PMID:19793923]"
/note="Member of the PAK family of serine/threonine
protein kinases; similar to Ste20p; involved in
down-regulation of sterol uptake; proposed to be a
downstream effector of Cdc42p during polarized growth;
SKM1 has a paralog, CLA4, that arose from the whole genome
duplication"
/codon_start=1
/product="putative serine/threonine protein kinase SKM1"
/protein_id="NP_014528.1"
/db_xref="GeneID:854036"
/db_xref="SGD:S000005473"
/translation="MKGVKKEGWISYKVDGLFSFLWQKRYLVLNDSYLAFYKSDKCNE
EPVLSVPLTSITNVSRIQLKQNCFEILRATDQKENISPINSYFYESNSKRSIFISTRT
ERDLHGWLDAIFAKCPLLSGVSSPTNFTHKVHVGFDPKVGNFVGVPDSWAKLLQTSEI
TYDDWNRNSKAVIKALQFYEDYNGLDTMQFNDHLNTSLDLKPLKSPTRYIINKRTNSI
KRSVSRTLRKGKTDSILPVYQSELKPFPRPSDDDYKFTNIEDNKVREEGRVHVSKEST
ADSQTKQLGKKEQKVIQSHLRRHDNNSTFRPHRLAPSAPATKNHDSKTKWHKEDLLEL
KNNDDSNEIIMKMKTVAIDVNPRPYFQLVEKAGQGASGAVYLSKRIKLPQENDPRFLK
SHCHRVVGERVAIKQIRLSEQPKKQLIMNELLVMNDSRQENIVNFLEAYIIDDEELWV
IMEYMEGGCLTDILDAVARSNTGEHSSPLNENQMAYIVKETCQGLKFLHNKKIIHRDI
KSDNILLNSQGLVKITDFGFCVELTEKRSKRATMVGTPYWMAPEIVNQKGYDEKVDVW
SLGIMLIEMIEGEPPYLNEDPLKALYLIANNGSPKLRHPESVSKQTKQFLDACLQVNV
ESRASVRKLLTFEFLSMACSPEQLKVSLKWH"
gene <106710..>108188
/gene="MSB4"
/locus_tag="YOL112W"
/db_xref="GeneID:854037"
mRNA <106710..>108188
/gene="MSB4"
/locus_tag="YOL112W"
/product="Rab GTPase-activating protein MSB4"
/transcript_id="NM_001183366.1"
/db_xref="GeneID:854037"
CDS 106710..108188
/gene="MSB4"
/locus_tag="YOL112W"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:10679030]"
/experiment="EXISTENCE:direct assay:GO:0000133 polarisome
[PMID:16166638]"
/experiment="EXISTENCE:direct assay:GO:0005096 GTPase
activator activity [PMID:10937877]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:10679030]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:35134079]"
/experiment="EXISTENCE:genetic interaction:GO:0006887
exocytosis [PMID:12913108]"
/experiment="EXISTENCE:genetic interaction:GO:0070649
formin-nucleated actin cable assembly [PMID:16166638]"
/experiment="EXISTENCE:physical interaction:GO:0070649
formin-nucleated actin cable assembly
[PMID:12913108|PMID:16166638]"
/note="GTPase-activating protein of the Ras superfamily;
acts primarily on Sec4p, localizes to the bud site and bud
tip; msb3 msb4 double mutation causes defects in secretion
and actin organization; similar to the TBC-domain Tre2
oncogene; MSB4 has a paralog, MSB3, that arose from the
whole genome duplication; human homolog USP6NL can
complement yeast msb3 msb4 double null mutant"
/codon_start=1
/product="Rab GTPase-activating protein MSB4"
/protein_id="NP_014529.1"
/db_xref="GeneID:854037"
/db_xref="SGD:S000005472"
/translation="MIMSSTMSTEAALVPNESVFDTVSSFNEDDANYSVLDLYDDDDE
GDDSSTVERKEILTTRELEKAKAFTSLIMADPENFDRYGFSKKGYFISQEEYDKWWTE
YNRYTERRKKKWENFLLKNKIELHNDNPLVYPARTDELSKFVRKGIPAEWRGNAWWYF
AGGQRQLDANVGVYDRLKSDCREGAVSGKDMEAIERDLYRTFPDNIHFHKESFQNGEP
AIIRSLRRVLMAFSVYDKTIGYCQSMNFLVGLLLLFMEEEKAFWMLVIITGKYLPGVY
ESDLEGANVDQGVLVLCIKEYLPEIWSHIESSYMNGNGSTDQISGPASGEEYLCRLPT
LTLCTASWFMSCFVGVVPIETTLRIWDCLFYEESHFLFKVALGILKLSESEFLESKSQ
KLFRQYSSYTFGGSNDSDSTFKRLKNKIKTQEEADMEILQVIQNFPKRLLNPNDIFEK
VLMKKKVALNGITQEKIDRGREYVAMARNRQRASSRPKERRK"
gene complement(<108258..>108896)
/gene="MDY2"
/locus_tag="YOL111C"
/gene_synonym="GET5; TMA24"
/db_xref="GeneID:854038"
mRNA complement(<108258..>108896)
/gene="MDY2"
/locus_tag="YOL111C"
/gene_synonym="GET5; TMA24"
/product="Mdy2p"
/transcript_id="NM_001183365.1"
/db_xref="GeneID:854038"
CDS complement(108258..108896)
/gene="MDY2"
/locus_tag="YOL111C"
/gene_synonym="GET5; TMA24"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:23285234|PMID:16390866]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006620
post-translational protein targeting to endoplasmic
reticulum membrane [PMID:20850366]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:23285234|PMID:26777405]"
/experiment="EXISTENCE:direct assay:GO:0030674
protein-macromolecule adaptor activity [PMID:17441508]"
/experiment="EXISTENCE:direct assay:GO:0072380 TRC complex
[PMID:20850366]"
/experiment="EXISTENCE:genetic interaction:GO:0045048
protein insertion into ER membrane [PMID:19325107]"
/experiment="EXISTENCE:mutant phenotype:GO:0000753 cell
morphogenesis involved in conjugation with cellular fusion
[PMID:16390866]"
/note="Protein involved in inserting tail-anchored
proteins into ER membranes; forms a complex with Get4p;
required for efficient mating; involved in shmoo formation
and nuclear migration in the pre-zygote; associates with
ribosomes"
/codon_start=1
/product="Mdy2p"
/protein_id="NP_014530.1"
/db_xref="GeneID:854038"
/db_xref="SGD:S000005471"
/translation="MSTSASGPEHEFVSKFLTLATLTEPKLPKSYTKPLKDVTNLGVP
LPTLKYKYKQNRAKKLKLHQDQQGQDNAAVHLTLKKIQAPKFSIEHDFSPSDTILQIK
QHLISEEKASHISEIKLLLKGKVLHDNLFLSDLKVTPANSTITVMIKPNPTISKEPEA
EKSTNSPAPAPPQELTVPWDDIEALLKNNFENDQAAVRQVMERLQKGWSLAK"
gene <109176..>109889
/gene="SHR5"
/locus_tag="YOL110W"
/gene_synonym="ERF4"
/db_xref="GeneID:854039"
mRNA <109176..>109889
/gene="SHR5"
/locus_tag="YOL110W"
/gene_synonym="ERF4"
/product="Shr5p"
/transcript_id="NM_001183364.1"
/db_xref="GeneID:854039"
CDS 109176..109889
/gene="SHR5"
/locus_tag="YOL110W"
/gene_synonym="ERF4"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0016409
palmitoyltransferase activity [PMID:12193598]"
/experiment="EXISTENCE:direct assay:GO:0018345 protein
palmitoylation [PMID:12193598]"
/experiment="EXISTENCE:direct assay:GO:0071561
nucleus-vacuole junction [PMID:41132095]"
/experiment="EXISTENCE:mutant phenotype:GO:0006612 protein
targeting to membrane [PMID:7532279]"
/experiment="EXISTENCE:mutant phenotype:GO:0018345 protein
palmitoylation [PMID:16751107]"
/experiment="EXISTENCE:physical interaction:GO:0031211
endoplasmic reticulum palmitoyltransferase complex
[PMID:12193598]"
/note="Subunit of a palmitoyltransferase (PAT) complex
with Erf2p; the PAT complex adds a palmitoyl lipid moiety
to heterolipidated substrates such as Ras1p and Ras2p
through a thioester linkage; required for palmitoylation,
membrane attachment and function of Ras2p; localizes to
the ER and the nucleus-vacuole junction"
/codon_start=1
/product="Shr5p"
/protein_id="NP_014531.1"
/db_xref="GeneID:854039"
/db_xref="SGD:S000005470"
/translation="MCDSHQKEEDNANTSERALFFNYHEFSYSFYEDLGSEDAKPTEH
DEDHKLCITHFPNVYAARGSAEFQVTRVVRVPRRFDESRSSLETPQFSTQLPGSEPAA
IVGDDGTSFVRCGRYDIGDHVFGCSSVSPLSEYLSAAELAEVVHRVNGFLLREEGEVF
GWRNLSGLLLDMLTGGLWSWVLGPLLSRPVFQESLALEQYVAQLNSPGGLLHERGVRL
VLPRRSGCLSLDFVVPRPK"
gene <110297..>110638
/gene="ZEO1"
/locus_tag="YOL109W"
/db_xref="GeneID:854040"
mRNA <110297..>110638
/gene="ZEO1"
/locus_tag="YOL109W"
/product="Zeo1p"
/transcript_id="NM_001183363.1"
/db_xref="GeneID:854040"
CDS 110297..110638
/gene="ZEO1"
/locus_tag="YOL109W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:16407407]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836|PMID:12949174]"
/experiment="EXISTENCE:mutant phenotype:GO:0031505
fungal-type cell wall organization [PMID:12949174]"
/experiment="EXISTENCE:mutant phenotype:GO:1903138
negative regulation of cell integrity MAPK cascade
[PMID:12949174]"
/note="Peripheral membrane protein of the plasma membrane;
interacts with Mid2p; regulates the cell integrity pathway
mediated by Pkc1p and Slt2p; the authentic protein is
detected in a phosphorylated state in highly purified
mitochondria"
/codon_start=1
/product="Zeo1p"
/protein_id="NP_014532.1"
/db_xref="GeneID:854040"
/db_xref="SGD:S000005469"
/translation="MSEIQNKAETAAQDVQQKLEETKESLQNKGQEVKEQAEASIDNL
KNEATPEAEQVKKEEQNIADGVEQKKTEAANKVEETKKQASAAVSEKKETKKEGGFLK
KLNRKIASIFN"
gene complement(110962..111033)
/gene="SUF1"
/locus_tag="YNCO0001C"
/db_xref="GeneID:854041"
tRNA complement(110962..111033)
/gene="SUF1"
/locus_tag="YNCO0001C"
/product="tRNA-Gly"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE
analysis; can mutate to suppress +1 frameshift mutations
in glycine codons"
/db_xref="GeneID:854041"
/db_xref="SGD:S000006592"
gene complement(<111431..>111886)
/gene="INO4"
/locus_tag="YOL108C"
/db_xref="GeneID:854042"
mRNA complement(<111431..>111886)
/gene="INO4"
/locus_tag="YOL108C"
/product="Ino4p"
/transcript_id="NM_001183362.1"
/db_xref="GeneID:854042"
CDS complement(111431..111886)
/gene="INO4"
/locus_tag="YOL108C"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:8195172]"
/experiment="EXISTENCE:direct assay:GO:0001228 DNA-binding
transcription activator activity, RNA polymerase
II-specific [PMID:8195172]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:15455074]"
/experiment="EXISTENCE:direct assay:GO:0045944 positive
regulation of transcription by RNA polymerase II
[PMID:8195172]"
/experiment="EXISTENCE:direct assay:GO:0090575 RNA
polymerase II transcription regulator complex
[PMID:8195172]"
/experiment="EXISTENCE:mutant phenotype:GO:0001228
DNA-binding transcription activator activity, RNA
polymerase II-specific [PMID:8195172]"
/experiment="EXISTENCE:mutant phenotype:GO:0008654
phospholipid biosynthetic process [PMID:2834106]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:1461729|PMID:8195172]"
/experiment="EXISTENCE:physical interaction:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:8195172]"
/experiment="EXISTENCE:physical interaction:GO:0090575 RNA
polymerase II transcription regulator complex
[PMID:8195172]"
/note="Transcription factor involved in phospholipid
synthesis; required for derepression of
inositol-choline-regulated genes involved in phospholipid
synthesis; forms a complex, with Ino2p, that binds the
inositol-choline-responsive element through a basic
helix-loop-helix domain"
/codon_start=1
/product="Ino4p"
/protein_id="NP_014533.1"
/db_xref="GeneID:854042"
/db_xref="SGD:S000005468"
/translation="MTNDIKEIQTIQPGLSEIKEIKGELANVKKRKRRSKKINKLTDG
QIRINHVSSEKKRRELERAIFDELVAVVPDLQPQESRSELIIYLKSLSYLSWLYERNE
KLRKQIIAKHEAKTGSSSSSDPVQEQNGNIRDLVPKELIWELGDGQSGQ"
gene <112102..>113130
/locus_tag="YOL107W"
/db_xref="GeneID:854043"
mRNA <112102..>113130
/locus_tag="YOL107W"
/product="uncharacterized protein"
/transcript_id="NM_001183361.1"
/db_xref="GeneID:854043"
CDS 112102..113130
/locus_tag="YOL107W"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0030137 COPI-coated
vesicle [PMID:14562095]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to the cytoplasm and
colocalizes in a punctate pattern with the early
golgi/COPI vesicles; YOL107W is not an essential protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014534.1"
/db_xref="GeneID:854043"
/db_xref="SGD:S000005467"
/translation="MQYSSRFLELNIPDSFLNINKIPDATKFITVTYICLTATLFCIR
RSLYNKLVLEDPNLDYNLITSPLLQMVPSQIWRYPTSLVLSNFIDTKAWKVVVNLLNL
IIGGSFIERNWNSSKEMFKFIIVLGSLTNVLIIMLTLLVSFFSNKVRLDIPLDGNYTI
LIGFPIIYRQLLPETTIIHLKTPQFLAKNFRFKLLPIFVMFTMTVTQIIWFHHFAQLF
SIWVTFFASWSYLRFFQKLAPLNCPSLPTTNSQGGQEILVGDASDTFQLIYFFPDLIK
PILRPIFNFIYNVVVVKFKVIKPFHDIDIDIGNTIAESRGAKKIMTVEERRRQLALQV
LEERMVNP"
repeat_region complement(113294..113626)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007150"
gene 113802..113874
/locus_tag="YNCO0002W"
/db_xref="GeneID:854045"
tRNA 113802..113874
/locus_tag="YNCO0002W"
/product="tRNA-Thr"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:854045"
/db_xref="SGD:S000006746"
rep_origin 113885..113983
/note="ARS1509; Autonomously Replicating Sequence"
/db_xref="SGD:S000118486"
gene complement(<114138..>115808)
/gene="WSC3"
/locus_tag="YOL105C"
/db_xref="GeneID:854046"
mRNA complement(<114138..>115808)
/gene="WSC3"
/locus_tag="YOL105C"
/product="Wsc3p"
/transcript_id="NM_001183359.1"
/db_xref="GeneID:854046"
CDS complement(114138..115808)
/gene="WSC3"
/locus_tag="YOL105C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:27337501]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807]"
/experiment="EXISTENCE:mutant phenotype:GO:0000425
pexophagy [PMID:20385774]"
/experiment="EXISTENCE:mutant phenotype:GO:0004888
transmembrane signaling receptor activity [PMID:10430578]"
/experiment="EXISTENCE:mutant phenotype:GO:0007266 Rho
protein signal transduction [PMID:10348843]"
/experiment="EXISTENCE:mutant phenotype:GO:0009408
response to heat [PMID:10348843]"
/note="Sensor-transducer of the stress-activated PKC1-MPK1
signaling pathway; involved in maintenance of cell wall
integrity; involved in response to heat shock and other
stressors; regulates 1,3-beta-glucan synthesis; WSC3 has a
paralog, WSC2, that arose from the whole genome
duplication"
/codon_start=1
/product="Wsc3p"
/protein_id="NP_014536.1"
/db_xref="GeneID:854046"
/db_xref="SGD:S000005465"
/translation="MERVWFAKLTNKGTIKIGYISFILLSLLCQSLIGLVNADFNYEG
CYSAADIQSAGLSLKNSYIYQSVSYCQNQCPESAVVALFNGSDCYCGNSVSFLTSLTK
STDSNCGTKCSGWPYQMCGGSSYMNVYVNAETFVSSVESSSSKEGSSTSYMPSTTSSL
SSAQISSTTRRTSTDMKSSEMIATTVSTTSTTSSSTSSTTSSTTSSTTSSTTSSTTSS
STSSTTSSTTSSTTSSTTSSTTSSTTSSTTSSTTSSTTSIFSVTSSSSSITLSSSEHT
TVDSRTSSPSSTLVPVSSSSSTLSTPKVTSMTPSTSSTIPIVTSVELVTSVVTKAIVS
TSDQHQETIFVTRTSVVERSSEVATATAAASNNRSNSTSKQRLSGGAIAGIVIGVVFG
VIFIILILLFLIWRRRKSHDQLDLEETKHYQPYSFGDEDANPIGPPPSSGTTNWMRHS
RGNTAGSIGTSNMYGFSMSNGANYSSPSSNTSGSIINNLAGLQDATVQKQNLPSTVFE
EANTLNSANERFSANSLPDMMMSGPLQVVNPDNPDNPELSSTVSHNRA"
gene complement(<116396..>117454)
/gene="NDJ1"
/locus_tag="YOL104C"
/gene_synonym="TAM1"
/db_xref="GeneID:854047"
mRNA complement(<116396..>117454)
/gene="NDJ1"
/locus_tag="YOL104C"
/gene_synonym="TAM1"
/product="Ndj1p"
/transcript_id="NM_001183358.1"
/db_xref="GeneID:854047"
CDS complement(116396..117454)
/gene="NDJ1"
/locus_tag="YOL104C"
/gene_synonym="TAM1"
/experiment="EXISTENCE:direct assay:GO:0000781 chromosome,
telomeric region
[PMID:25897084|PMID:9242487|PMID:9157883]"
/experiment="EXISTENCE:direct assay:GO:0007129 homologous
chromosome pairing at meiosis [PMID:9157883]"
/experiment="EXISTENCE:direct assay:GO:0035974 meiotic
spindle pole body [PMID:25897084]"
/experiment="EXISTENCE:direct assay:GO:0045141 meiotic
telomere clustering [PMID:12734403]"
/experiment="EXISTENCE:genetic interaction:GO:0007129
homologous chromosome pairing at meiosis [PMID:17495028]"
/experiment="EXISTENCE:mutant phenotype:GO:0007129
homologous chromosome pairing at meiosis
[PMID:9157883|PMID:17495028]"
/experiment="EXISTENCE:mutant phenotype:GO:0007131
reciprocal meiotic recombination [PMID:16648465]"
/experiment="EXISTENCE:mutant phenotype:GO:0010520
regulation of reciprocal meiotic recombination
[PMID:18818742|PMID:18818741]"
/experiment="EXISTENCE:mutant phenotype:GO:0045141 meiotic
telomere clustering [PMID:11018056]"
/experiment="EXISTENCE:mutant phenotype:GO:0070197 meiotic
attachment of telomere to nuclear envelope
[PMID:18818742|PMID:18818741]"
/experiment="EXISTENCE:mutant phenotype:GO:2000711
positive regulation of maintenance of meiotic sister
chromatid cohesion, centromeric [PMID:25897084]"
/note="Protein that regulates meiotic SPB cohesion and
telomere clustering; localizes to both spindle pole bodies
(SPBs) and telomeres; required for bouquet formation,
effective homolog pairing, ordered cross-over
distribution, sister chromatid cohesion at meiotic
telomeres, chromosomal segregation and telomere-led rapid
prophase movement"
/codon_start=1
/product="Ndj1p"
/protein_id="NP_014537.1"
/db_xref="GeneID:854047"
/db_xref="SGD:S000005464"
/translation="MSKDNRLASILLQPVASSSGNCTEFHDSKLHTLQEELNFLPLEG
VASNVCPPMFRGHKNYVFVLYCLNQVDLVTNLQDSTKRYYPLQIFKDCQLSSLVQKDF
SHYFQLSRQKEGEDRNDSDTTLVNVVNSGVSRHRSQLLKMCIIPRICSFDKSNSKTYK
LIQEYVNRFETVLTKFGPEKDFTKVYANWSKLIESFNELILHDLLVKWQQWSELTQPN
ATVHQNIPNVLRELVIKLTQRYFTFQPSYSCSIDEFTTILLNKNALSLLDVFRKPRKY
KLNFGLWLDCQNGILIFTNGIVQMADEITSERVKSFVRPAHLLVLEDHSNDEAVKKLM
FFTFSAILQCFTDEILNC"
mobile_element 117703..123628
/note="YOLWTy1-1; Ty1 element, LTR retrotransposon of the
Copia (Pseudoviridae) group; contains co-transcribed genes
TYA Gag and TYB Pol, encoding proteins involved in
structure and function of virus-like particles, flanked by
two direct repeats"
/mobile_element_type="retrotransposon:YOLWTy1-1"
/db_xref="SGD:S000007161"
repeat_region 117703..118040
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007154"
gene <118000..>123268
/locus_tag="YOL103W-B"
/db_xref="GeneID:854049"
mRNA <118000..>123268
/locus_tag="YOL103W-B"
/product="gag-pol fusion protein"
/transcript_id="NM_001184384.2"
/db_xref="GeneID:854049"
CDS join(118000..119304,119306..123268)
/locus_tag="YOL103W-B"
/EC_number="2.7.7.7"
/EC_number="2.7.7.49"
/EC_number="3.1.26.4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9448009]"
/ribosomal_slippage
/note="Retrotransposon TYA Gag and TYB Pol genes;
transcribed/translated as one unit; polyprotein is
processed to make a nucleocapsid-like protein (Gag),
reverse transcriptase (RT), protease (PR), and integrase
(IN); similar to retroviral genes"
/codon_start=1
/product="gag-pol fusion protein"
/protein_id="NP_058181.1"
/db_xref="GeneID:854049"
/db_xref="SGD:S000007350"
/translation="MESQQLSNYPQISHGSACASVTSKEVHTNQDPLDVSASKTEECE
KASTKANSQQTTTPASSAVPENPHHASPQPASVPPPQNGPYPQQCMMTQNQANPSGWS
FYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVRQITDDELT
FLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQ
YNGSTPADAFETKVTNIINRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNM
TVAELFLDIHAIYEEQQGSRNSKPNYRRNLSDEKNDSRSYTNTTKPKVIARNPQKTNN
SKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLGQELTESTVNHTNHS
DDELPGHLLLDSGASRTLIRSAHHIHSASSNPDINVVDAQKRNIPINAIGDLQFHFQD
NTKTSIKVLHTPNIAYDLLSLNELAAVDITACFTKNVLERSDGTVLAPIVKYGDFYWV
SKKYLLPSNISVPTINNVHTSESTRKYPYPFIHRMLAHANAQTIRYSLKNNTITYFNE
SDVDWSSAIDYQCPDCLIGKSTKHRHIKGSRLKYQNSYEPFQYLHTDIFGPVHNLPKS
APSYFISFTDETTKFRWVYPLHDRREDSILDVFTTILAFIKNQFQASVLVIQMDRGSE
YTNRTLHKFLEKNGITPCYTTTADSRAHGVAERLNRTLLDDCRTQLQCSGLPNHLWFS
AIEFSTIVRNSLASPKSKKSARQHAGLAGLDISTLLPFGQPVIVNDHNPNSKIHPRGI
PGYALHPSRNSYGYIIYLPSLKKTVDTTNYVILQGKESRLDQFNYDALTFDEDLNRLT
ASYQSFIASNEIQQSDDLNIESDHDFQSDIELHPEQPRNVLSKAVSPTDSTPPSTHTE
DSKRVSKTNIRAPREVDPNISESNILPSKKRSSTPQISNIESTGSGGMHKLNVPLLAP
MSQSNTHESSHASKSKDFRHSDSYSENETNHTNVPISSTGGTNNKTVPQISDQETEKR
IIHRSPSIDASPPENNSSHNIVPIKTPTTVSEQNTEESIIADLPLPDLPPESPTEFPD
PFKELPPINSHQTNSSLGGIGDSNAYTTINSKKRSLEDNETEIKVSRDTWNTKNMRSL
EPPRSKKRIHLIAAVKAVKSIKPIRTTLRYDEAITYNKDIKEKEKYIEAYHKEVNQLL
KMKTWDTDEYYDRKEIDPKRVINSMFIFNKKRDGTHKARFVARGDIQHPDTYDSGMQS
NTVHHYALMTSLSLALDNNYYITQLDISSAYLYADIKEELYIRPPPHLGMNDKLIRLK
KSLYGLKQSGANWYETIKSYLIKQCGMEEVRGWSCVFKNSQVTICLFVDDMILFSKDL
NANKKIITTLKKQYDTKIINLGESDNEIQYDILGLEIKYQRGKYMKLGMENSLTEKIP
KLNVPLNPKGRKLSAPGQPGLYIDQDELEIDEDEYKEKVHEMQKLIGLASYVGYKFRF
DLLYYINTLAQHILFPSRQVLDMTYELIQFMWDTRDKQLIWHKNKPTEPDNKLVAISD
ASYGNQPYYKSQIGNIYLLNGKVIGGKSTKASLTCTSTTEAEIHAISESVPLLNNLSY
LIQELNKKPIIKGLLTDSRSTISIIKSTNEEKFRNRFFGTKAMRLRDEVSGNNLYVYY
IETKKNIADVMTKPLPIKTFKLLTNKWIH"
gene <118000..>119322
/locus_tag="YOL103W-A"
/db_xref="GeneID:854048"
mRNA <118000..>119322
/locus_tag="YOL103W-A"
/product="gag protein"
/transcript_id="NM_001184383.1"
/db_xref="GeneID:854048"
CDS 118000..119322
/locus_tag="YOL103W-A"
/note="Retrotransposon TYA Gag gene co-transcribed with
TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is
a nucleocapsid protein that is the structural constituent
of virus-like particles (VLPs); similar to retroviral Gag"
/codon_start=1
/product="gag protein"
/protein_id="NP_058182.1"
/db_xref="GeneID:854048"
/db_xref="SGD:S000007349"
/translation="MESQQLSNYPQISHGSACASVTSKEVHTNQDPLDVSASKTEECE
KASTKANSQQTTTPASSAVPENPHHASPQPASVPPPQNGPYPQQCMMTQNQANPSGWS
FYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVRQITDDELT
FLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQ
YNGSTPADAFETKVTNIINRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNM
TVAELFLDIHAIYEEQQGSRNSKPNYRRNLSDEKNDSRSYTNTTKPKVIARNPQKTNN
SKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLRPGTY"
repeat_region 123291..123628
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007155"
gene <124001..>125830
/gene="ITR2"
/locus_tag="YOL103W"
/gene_synonym="HRB612"
/db_xref="GeneID:854050"
mRNA <124001..>125830
/gene="ITR2"
/locus_tag="YOL103W"
/gene_synonym="HRB612"
/product="myo-inositol transporter ITR2"
/transcript_id="NM_001183357.1"
/db_xref="GeneID:854050"
CDS 124001..125830
/gene="ITR2"
/locus_tag="YOL103W"
/gene_synonym="HRB612"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16233524]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0005365
myo-inositol transmembrane transporter activity
[PMID:2040626]"
/experiment="EXISTENCE:mutant phenotype:GO:0015798
myo-inositol transport [PMID:2040626]"
/note="Myo-inositol transporter; member of the sugar
transporter superfamily; expressed constitutively; ITR2
has a paralog, ITR1, that arose from the whole genome
duplication"
/codon_start=1
/product="myo-inositol transporter ITR2"
/protein_id="NP_014538.2"
/db_xref="GeneID:854050"
/db_xref="SGD:S000005463"
/translation="MKNSTAASSRWTKSRLSHFFPSYTNSSGMGAASTDQSSTQGEEL
HHRKHCEEDNDGQKPKKSPVSTSTMQIKSRQDEDEDDGRIVIKPVNDEDDTSVIITFN
QSISPFIITLTFVASISGFMFGYDTGYISSALISINRDLDNKVLTYGEKELITAATSL
GALITSVGAGTAADVFGRRPCLMFSNLMFLIGAILQITAHKFWQMAAGRLIMGFGVGI
GSLISPLFISEIAPKMIRGRLTVINSLWLTGGQLIAYGCGAGLNHVKNGWRILVGLSL
IPTVLQFSFFCFLPDTPRYYVMKGDLKRAKMVLKRSYVNTEDEIIDQKVEELSSLNQS
IPGKNPITKFWNMVKELHTVPSNFRALIIGCGLQAIQQFTGWNSLMYFSGTIFETVGF
KNSSAVSIIVSGTNFVFTLIAFFCIDKIGRRYILLIGLPGMTVALVICAIAFHFLGIK
FNGADAVVASDGFSSWGIVIIVFIIVYAAFYALGIGTVPWQQSELFPQNVRGVGTSYA
TATNWAGSLVIASTFLTMLQNITPTGTFSFFAGVACLSTIFCYFCYPELSGLELEEVQ
TILKDGFNIKASKALAKKRKQQVAEGAAHHKLKFEPTQEIVES"
gene complement(<125997..>126689)
/gene="TPT1"
/locus_tag="YOL102C"
/db_xref="GeneID:854051"
mRNA complement(<125997..>126689)
/gene="TPT1"
/locus_tag="YOL102C"
/product="tRNA 2'-phosphotransferase"
/transcript_id="NM_001183356.1"
/db_xref="GeneID:854051"
CDS complement(125997..126689)
/gene="TPT1"
/locus_tag="YOL102C"
/EC_number="2.7.1.160"
/experiment="EXISTENCE:direct assay:GO:0000215 tRNA
2'-phosphotransferase activity [PMID:9148937]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22391451]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22391451]"
/experiment="EXISTENCE:direct assay:GO:0006388 tRNA
splicing, via endonucleolytic cleavage and ligation
[PMID:9148937]"
/note="tRNA 2'-phosphotransferase; catalyzes the final
step in tRNA splicing, transferring the 2'-PO(4) from the
splice junction to NAD(+), forming ADP-ribose
1''-2''cyclic phosphate and nicotinamide;
ADP-ribosyltransferase that ADP-ribosylates
5'-phosphorylated ssDNA and ssRNAs; human and mouse
orthologs complement the inviability of the yeast null
mutant"
/codon_start=1
/product="tRNA 2'-phosphotransferase"
/protein_id="NP_014539.1"
/db_xref="GeneID:854051"
/db_xref="SGD:S000005462"
/translation="MRQVLQKDKRDVQLSKALSYLLRHTAVKEKLTIDSNGYTPLKEL
LSHNRLKTHKCTVDDIHRIVKENDKQRFHIKTLGADEEWICATQGHSIKSIQPSDEVL
VPITEASQLPQELIHGTNLQSVIKIIESGAISPMSRNHVHLSPGMLHAKGVISGMRSS
SNVYIFIDCHSPLFFQTLKMFRSLNNVYLSSSIPVELIQKVVVKGNLKDEEKLDTLRR
ILHERNIPLEKI"
gene complement(<126982..>127920)
/gene="IZH4"
/locus_tag="YOL101C"
/db_xref="GeneID:854052"
mRNA complement(<126982..>127920)
/gene="IZH4"
/locus_tag="YOL101C"
/product="Izh4p"
/transcript_id="NM_001183355.1"
/db_xref="GeneID:854052"
CDS complement(126982..127920)
/gene="IZH4"
/locus_tag="YOL101C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:mutant phenotype:GO:0006882
intracellular zinc ion homeostasis [PMID:15060275]"
/note="Membrane protein involved in zinc ion homeostasis;
member of the four-protein IZH family; expression induced
by fatty acids and altered zinc levels; deletion reduces
sensitivity to excess zinc; possible role in sterol
metabolism; protein increases in abundance and relocalizes
from nucleus to ER upon DNA replication stress; IZH4 has a
paralog, IZH1, that arose from the whole genome
duplication"
/codon_start=1
/product="Izh4p"
/protein_id="NP_014540.1"
/db_xref="GeneID:854052"
/db_xref="SGD:S000005461"
/translation="MVSLTTIEQSPVKCETTTEKESNDTRGTDSNENAETKETKKGFP
FHDLAKLQKQYKNKSSRNESLVALIYLLGSMLSFCLLIFFTDFYLIPLFPTTTTMTDY
IVFNFYLLNVFVFCMVHFIYHFVKNISLQQHLEHWQKFSYLSNINLLISSQITILYYL
FYDYVFFFKIFTLLMNFIGLVAYFFILTDKLISSKRFNKTVFFISVSVVCCSLPLLTA
IITFDGLENLKERIKVNAITWELVALVAASIIYVTRFPESLFRRNKKEEGWNHSEYLF
HLLISGTAFYHFFILIQSYILMHSSLNQPELINFKS"
gene <129237..>132482
/gene="PKH2"
/locus_tag="YOL100W"
/db_xref="GeneID:854053"
mRNA <129237..>132482
/gene="PKH2"
/locus_tag="YOL100W"
/product="serine/threonine protein kinase PKH2"
/transcript_id="NM_001183354.1"
/db_xref="GeneID:854053"
CDS 129237..132482
/gene="PKH2"
/locus_tag="YOL100W"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894|PMID:10567559]"
/experiment="EXISTENCE:direct assay:GO:0004674 protein
serine/threonine kinase activity [PMID:22918958]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12221112]"
/experiment="EXISTENCE:direct assay:GO:0005938 cell cortex
[PMID:12221112]"
/experiment="EXISTENCE:direct assay:GO:0032126 eisosome
[PMID:37387118]"
/experiment="EXISTENCE:genetic interaction:GO:0000196 cell
integrity MAPK cascade [PMID:10567559]"
/experiment="EXISTENCE:genetic interaction:GO:0010606
positive regulation of cytoplasmic mRNA processing body
assembly [PMID:21163942]"
/experiment="EXISTENCE:genetic interaction:GO:0032511 late
endosome to vacuole transport via multivesicular body
sorting pathway [PMID:22918958]"
/experiment="EXISTENCE:genetic interaction:GO:0060211
regulation of nuclear-transcribed mRNA poly(A) tail
shortening [PMID:21163942]"
/experiment="EXISTENCE:mutant phenotype:GO:0000196 cell
integrity MAPK cascade [PMID:10567559]"
/experiment="EXISTENCE:mutant phenotype:GO:0032511 late
endosome to vacuole transport via multivesicular body
sorting pathway [PMID:22918958]"
/note="Serine/threonine protein kinase; involved in
sphingolipid-mediated signaling pathway that controls
endocytosis; activates Ypk1p and Ykr2p, components of
signaling cascade required for maintenance of cell wall
integrity; contains a PH-like domain; redundant with
Pkh1p; PKH2 has a paralog, PKH1, that arose from the whole
genome duplication"
/codon_start=1
/product="serine/threonine protein kinase PKH2"
/protein_id="NP_014541.1"
/db_xref="GeneID:854053"
/db_xref="SGD:S000005460"
/translation="MYFDKDNSMSPRPLLPSDEQKLNINLLTKKEKFSHLDPHYDAKA
TPQRSTSNRNVGDLLLEKRTAKPMIQKALTNTDNFIEMYHNQQRKNLDDDTIKEVMIN
DENGKTVASTNDGRYDNDYDNNDINDQKTLDNIAGSPHMEKNRNKVKIEHDSSSQKPI
AKESSKAQKNIIKKGIKDFKFGSVIGDGAYSTVMLATSIDTKKRYAAKVLNKEYLIRQ
KKVKYVSIEKTALQKLNNSPSVVRLFSTFQDESSLYFLLEYAPNGDFLSLMKKYGSLD
ETCARYYAAQIIDAIDYLHSNGIIHRDIKPENILLDGEMKIKLTDFGTAKLLNPTNNS
VSKPEYDLSTRSKSFVGTAEYVSPELLNDSFTDYRCDIWAFGCILFQMIAGKPPFKAT
NEYLTFQKVMKVQYAFTPGFPLIIRDLVKKILVKNLDRRLTISQIKEHHFFKDLNFKD
GSVWSKTPPEIKPYKINAKSMQAMPSGSDRKLVKKSVNTLGKSHLVTQRSASSPSVEE
TTHSTLYNNNTHASTESEISIKKRPTDERTAQILENARKGINNRKNQPGKRTPSGAAS
AALAASAALTKKTMQSYPTSSSKSSRSSSPATTSRPGTYKRTSSTESKPFAKSPPLSA
SVLSSKVPMPPYTPPMSPPMTPYDTYQMTPPYTTKQQDYSDTAIAAPKPCISKQNVKN
STDSPLMNKQDIQWSFYLKNINEHVLRTEKLDFVTTNYDILEKKMLKLNGSLLDPQLF
GKPRHTFLSQVARSGGEVTGFRNDPTMTAYSKTEDTYYSKNIIDLQLLEDDYRIEGGD
LSELLTNRSGEGYKCNQNSSPMKDDDKSESNNKGSSVFSGKIKKLFHPTSAAETLSSS
DEKTKYYKRTIVMTSFGRFLVFAKRRQPNPVTNLKYELEYDINLRQQGTKIKELIIPL
EMGTNHIVVIQTPYKSFLLSTDKKTTSKLFTVLKKILNSNTNKIEKELLQRNQKVIER
RTSSSGRAIPKDLPTSKSPSPKPRTHSQSPSISKHNSFSESINSAKSNRSSRIFETFI
NAKEQNSKKHAAPVPLTSKLVNGLPKRQVTVGLGLNTGTNFKNSSAKSKRS"
gene complement(<132725..>135838)
/gene="SDD3"
/locus_tag="YOL098C"
/db_xref="GeneID:854055"
mRNA complement(<132725..>135838)
/gene="SDD3"
/locus_tag="YOL098C"
/product="Sdd3p"
/transcript_id="NM_001183352.1"
/db_xref="GeneID:854055"
CDS complement(132725..135838)
/gene="SDD3"
/locus_tag="YOL098C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="Putative metalloprotease; overproduction suppresses
lethality due to expression of the dominant PET9 allele
AAC2-A128P"
/codon_start=1
/product="Sdd3p"
/protein_id="NP_014543.1"
/db_xref="GeneID:854055"
/db_xref="SGD:S000005458"
/translation="MGFKKLVSFQPDYVPQYHITKYISERTKLQLVHINHKTSPLVHG
YFAVPTECLNDSGAPHTLEHLIFMGSKSYPYKGLLDTAGNLSLSNTNAWTDTDQTVYT
LSSAGWKGFSKLLPAYLDHILHPTLTDEACLTEVYHIDPENLGDKGVVFSEMEAIETQ
GWYISGLEKQRLMFPEGSGYRSETGGLTKNLRTLTNDEIRQFHKSLYSSDNLCVIVCG
NVPTDELLTVMEEWDNKLPEIPSNIPKKRPFLDNKLSHIPQSRDKVTESTVEFPELDE
SQGELLFSWIGVPYSDFRNDLAVDVLLDYFTDSALAVFTRELVEIDDPMANSTDCCTD
YFMRTIIDLRIQGVPTEKIAATKTKVLEILKTHTIDLSRVRQVVENTKWEYLLNYEKN
GESRFSSAVITDYIYGNEDGSSLVSSLKDLSDFDALLQWSQKDWQSLLNRIFVDNKPI
IVTAKPSALMYEQLEKEKSDLIKQREAEFDDEKKLVLLKRLNNAKNINDRPIPKSLLQ
KFEIDNPSKSVEFVNTKSIATVDSYKYNNVSDPLTKKILETRPDNFPLFIHLNHFPSQ
FIELHFLVNSASIKDTSLLPYFNMFDELFSMPMKILDEESNVETMLSFEEVVAKLKSE
TIDAQINQGLKGSCPDLINFKIQCRAGGYSNSVQWIKHCLFDMVFDENRVRILLENYL
NSIVEWKRNGNVMLSSLTNRNLYSARSLKKSTDPLFVEAKLQEIFAEIENGNFEKEIL
PRIETMRKQLRANFNKFHILVLGDISKIDDVYEPWNPLIKCLNIAHPVEKLKIPPVPR
ALDTISSICRTPGEKAFIITTPASESAYMNLITSIPFNLDYHDPEYAIVSLASEYLEC
VEGPFWKGIRGAGLAYGASMLKLCEINSWGFNIYRGADIIKCYEVGKQIVQDYASGAL
EFDEQLIQGAISSIINRLATIECGYFETALSKYVDEFCLQRGNNFNELYLERLQNVTK
TDLKNAMQKYFVNMFDSNKSVAFVSCHPAKLESVQEFFETQGFTVEIEELEDDDDEID
SEEDENA"
gene complement(136088..136183)
/gene="SNR58"
/locus_tag="YNCO0003C"
/db_xref="GeneID:9164976"
ncRNA complement(136088..136183)
/ncRNA_class="snoRNA"
/gene="SNR58"
/locus_tag="YNCO0003C"
/product="SNR58"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:10024243]"
/experiment="EXISTENCE:curator inference:GO:0031428 box
C/D methylation guide snoRNP complex [PMID:10024243]"
/experiment="EXISTENCE:mutant phenotype:GO:0030562 rRNA
2'-O-ribose methylation guide activity [PMID:10024243]"
/experiment="EXISTENCE:mutant phenotype:GO:0031167 rRNA
methylation [PMID:10024243]"
/note="C/D box small nucleolar RNA (snoRNA); guides
2'-O-methylation of large subunit (LSU) rRNA at position
C663"
/transcript_id="NR_132255.1"
/db_xref="GeneID:9164976"
/db_xref="SGD:S000006449"
gene <136220..>136405
/locus_tag="YOL097W-A"
/db_xref="GeneID:1466475"
mRNA <136220..>136405
/locus_tag="YOL097W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184665.1"
/db_xref="GeneID:1466475"
CDS 136220..136405
/locus_tag="YOL097W-A"
/note="hypothetical protein; identified by expression
profiling and mass spectrometry"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878163.1"
/db_xref="GeneID:1466475"
/db_xref="SGD:S000028854"
/translation="MQSMICSSEHENLTCKYWPVSFLASWCENGSGTLMQKDGSLLYA
VKNFSHIFEKKIFHTNL"
gene complement(<136527..>137825)
/gene="WRS1"
/locus_tag="YOL097C"
/gene_synonym="HRE342"
/db_xref="GeneID:854056"
mRNA complement(<136527..>137825)
/gene="WRS1"
/locus_tag="YOL097C"
/gene_synonym="HRE342"
/product="tryptophan--tRNA ligase WRS1"
/transcript_id="NM_001183351.1"
/db_xref="GeneID:854056"
CDS complement(136527..137825)
/gene="WRS1"
/locus_tag="YOL097C"
/gene_synonym="HRE342"
/EC_number="6.1.1.2"
/experiment="EXISTENCE:direct assay:GO:0004830
tryptophan-tRNA ligase activity [PMID:9046085]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:1990825
sequence-specific mRNA binding [PMID:34039240]"
/note="Cytoplasmic tryptophanyl-tRNA synthetase;
aminoacylates tryptophanyl-tRNA; human homolog WARS can
complement yeast null mutant"
/codon_start=1
/product="tryptophan--tRNA ligase WRS1"
/protein_id="NP_014544.1"
/db_xref="GeneID:854056"
/db_xref="SGD:S000005457"
/translation="MSNDETVEKVTQQVSELKSTDVKEQVVTPWDVEGGVDEQGRAQN
IDYDKLIKQFGTKPVNEETLKRFKQVTGREPHHFLRKGLFFSERDFTKILDLYEQGKP
FFLYTGRGPSSDSMHLGHMIPFVFTKWLQEVFDVPLVIELTDDEKFLFKHKLTINDVK
NFARENAKDIIAVGFDPKNTFIFSDLQYMGGAFYETVVRVSRQITGSTAKAVFGFNDS
DCIGKFHFASIQIATAFPSSFPNVLGLPDKTPCLIPCAIDQDPYFRVCRDVADKLKYS
KPALLHSRFFPALQGSTTKMSASDDTTAIFMTDTPKQIQKKINKYAFSGGQVSADLHR
ELGGNPDVDVAYQYLSFFKDDDVFLKECYDKYKSGELLSGEMKKLCIETLQEFVKAFQ
ERRAQVDEETLDKFMVPHKLVWGEKERLVAPKPKTKQEKK"
gene complement(<138107..>139045)
/gene="COQ3"
/locus_tag="YOL096C"
/db_xref="GeneID:854057"
mRNA complement(<138107..>139045)
/gene="COQ3"
/locus_tag="YOL096C"
/product="hexaprenyldihydroxybenzoate methyltransferase"
/transcript_id="NM_001183350.1"
/db_xref="GeneID:854057"
CDS complement(138107..139045)
/gene="COQ3"
/locus_tag="YOL096C"
/EC_number="2.1.1.64"
/EC_number="2.1.1.114"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:10760477|PMID:16823961|PMID:14576278|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:10419476]"
/experiment="EXISTENCE:direct assay:GO:0006744 ubiquinone
biosynthetic process [PMID:10419476]"
/experiment="EXISTENCE:mutant phenotype:GO:0006744
ubiquinone biosynthetic process [PMID:1885593]"
/experiment="EXISTENCE:mutant phenotype:GO:0010420
polyprenyldihydroxybenzoate methyltransferase activity
[PMID:1885593]"
/experiment="EXISTENCE:mutant phenotype:GO:0061542
3-demethylubiquinol 3-O-methyltransferase activity
[PMID:10419476]"
/note="O-methyltransferase; catalyzes two different
O-methylation steps in ubiquinone (Coenzyme Q)
biosynthesis; component of a mitochondrial
ubiquinone-synthesizing complex; phosphoprotein"
/codon_start=1
/product="hexaprenyldihydroxybenzoate methyltransferase"
/protein_id="NP_014545.2"
/db_xref="GeneID:854057"
/db_xref="SGD:S000005456"
/translation="MLLRSRFLKVIHVRKQLSACSRFAIQTQTRCKSTDASEDEVKHF
QELAPTWWDTDGSQRILHKMNLTRLDFVQRTVRNQVKIQNPEIFVPGFNYKEFLPEYV
CDNIQREMQESIETNLDKRPEVSVLDVGCGGGILSESLARLKWVKNVQGIDLTRDCIM
VAKEHAKKDPMLEGKINYECKALEDVTGQFDIITCMEMLEHVDMPSEILRHCWSRLNP
EKGILFLSTINRDLISWFTTIFMGENVLKIVPKGTHHLSKYINSKEILAWFNDNYSGQ
FRLLDLKGTMYLPYQGWVEHDCSDVGNYFMAIQRLN"
gene complement(<139227..>141347)
/gene="HMI1"
/locus_tag="YOL095C"
/db_xref="GeneID:854058"
mRNA complement(<139227..>141347)
/gene="HMI1"
/locus_tag="YOL095C"
/product="ATP-dependent 3'-5' DNA helicase"
/transcript_id="NM_001183349.1"
/db_xref="GeneID:854058"
CDS complement(139227..141347)
/gene="HMI1"
/locus_tag="YOL095C"
/EC_number="5.6.2.4"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:10409639]"
/experiment="EXISTENCE:direct assay:GO:0043138 3'-5' DNA
helicase activity [PMID:16358299]"
/experiment="EXISTENCE:mutant phenotype:GO:0032042
mitochondrial DNA metabolic process [PMID:16358299]"
/note="Mitochondrial inner membrane localized
ATP-dependent DNA helicase; required for the maintenance
of the mitochondrial genome; not required for
mitochondrial transcription; has homology to E. coli
helicase uvrD"
/codon_start=1
/product="ATP-dependent 3'-5' DNA helicase"
/protein_id="NP_014546.1"
/db_xref="GeneID:854058"
/db_xref="SGD:S000005455"
/translation="MDKLTPSQWKVINKSYEPASTIKVIAGPGSGKTLTLLYKVLHLI
TVENIKPEEILIFSLTNKAVDSIIENLLSIFENSHTNKEIVHQIGCYTVHGLANRIVV
ENEGMINIIEEIGWRGLMKLLPPSKRTPHHFRSYKELEKVVKDYKLNNAKNNNPVIEK
LVELMDNCKVMTNDDLIIRAKKYLELDSSDSDASSFTQDLRNKYKVVLIDEFQDLYPS
LAPLITMICKGKQLIMFGDTNQSIYGFLGSNNEIMSQLDNLHPKNSTTVLKLFDNFRS
TPEIISLASKIINRPLAEKQIIDDTDETPSELVRKLPSGVSPQIMTFDDLAAESEFII
DKITQLICSSAKFSDIAILSRTNSHLTAIASILKKYGIPYQKLKSQPDWMDDLRIQFL
LDILKVCSLASDEKHNREFNTGDKWQSNFSILVTMSALKGIGDASIQALYKACSLKNL
SIWKYLTMVPNFEWPLGLSIKKKMENYTSNLYEMIENDQVHQLDDPMELLEKVASITN
NLNLNPTYFQSLSDAQSSLEFKTHLQEMAQVMKVSKSNKPPGISFVKWFLETYFDQTM
VFHQSQQALQTTGPGTVKLSTIHSAKGLEFPIVFLTNGSMSNFPMDTNALYVGITRAR
NLLYMCNMKHERLVSKSSPYSRNIMSNNLFWTYYNKDLKRSVCDVKVTHGYNVQRYNQ
LRKNFGFYRAYSSLRGCKSVFRRI"
gene complement(<141584..>142555)
/gene="RFC4"
/locus_tag="YOL094C"
/db_xref="GeneID:854059"
mRNA complement(<141584..>142555)
/gene="RFC4"
/locus_tag="YOL094C"
/product="replication factor C subunit 4"
/transcript_id="NM_001183348.1"
/db_xref="GeneID:854059"
CDS complement(141584..142555)
/gene="RFC4"
/locus_tag="YOL094C"
/experiment="EXISTENCE:direct assay:GO:0003689 DNA clamp
loader activity [PMID:12604797|PMID:8995429]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005663 DNA
replication factor C complex [PMID:8063832]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0006272 leading
strand elongation [PMID:1346062]"
/experiment="EXISTENCE:direct assay:GO:0007062 sister
chromatid cohesion [PMID:11389843]"
/experiment="EXISTENCE:direct assay:GO:0031389 Rad17
RFC-like complex [PMID:12604797]"
/experiment="EXISTENCE:physical interaction:GO:0031389
Rad17 RFC-like complex [PMID:10660302]"
/experiment="EXISTENCE:physical interaction:GO:0031390
Ctf18 RFC-like complex [PMID:11389843]"
/experiment="EXISTENCE:physical interaction:GO:0031391
Elg1 RFC-like complex [PMID:12909721|PMID:12912927]"
/note="Subunit of heteropentameric Replication factor C
(RF-C); which is a DNA binding protein and ATPase that
acts as a clamp loader of the proliferating cell nuclear
antigen (PCNA) processivity factor for DNA polymerases
delta and epsilon; relocalizes to the cytosol in response
to hypoxia"
/codon_start=1
/product="replication factor C subunit 4"
/protein_id="NP_014547.1"
/db_xref="GeneID:854059"
/db_xref="SGD:S000005454"
/translation="MSKTLSLQLPWVEKYRPQVLSDIVGNKETIDRLQQIAKDGNMPH
MIISGMPGIGKTTSVHCLAHELLGRSYADGVLELNASDDRGIDVVRNQIKHFAQKKLH
LPPGKHKIVILDEADSMTAGAQQALRRTMELYSNSTRFAFACNQSNKIIEPLQSRCAI
LRYSKLSDEDVLKRLLQIIKLEDVKYTNDGLEAIIFTAEGDMRQAINNLQSTVAGHGL
VNADNVFKIVDSPHPLIVKKMLLASNLEDSIQILRTDLWKKGYSSIDIVTTSFRVTKN
LAQVKESVRLEMIKEIGLTHMRILEGVGTYLQLASMLAKIHKLNNKA"
gene <142815..>143696
/gene="TRM10"
/locus_tag="YOL093W"
/db_xref="GeneID:854060"
mRNA <142815..>143696
/gene="TRM10"
/locus_tag="YOL093W"
/product="tRNA (guanine(9)-N(1))-methyltransferase"
/transcript_id="NM_001183347.1"
/db_xref="GeneID:854060"
CDS 142815..143696
/gene="TRM10"
/locus_tag="YOL093W"
/EC_number="2.1.1.221"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0016423 tRNA
(guanine) methyltransferase activity [PMID:12702816]"
/experiment="EXISTENCE:direct assay:GO:0030488 tRNA
methylation [PMID:12702816]"
/note="tRNA methyltransferase; methylates N-1 of guanine
at position 9 in tRNAs; involved in methylation of
guanosine and uridine ribonucleosides in mRNA; involved in
distinct tRNA quality control pathway for tRNA-Trp; member
of SPOUT (SpoU-TrmD) methyltransferase family; human
ortholog TRMT10A plays role in pathogenesis of
microcephaly and early onset diabetes; an 18-mer ncRNA
translational regulator originates from TRM10 locus;
protein abundance increases in response to DNA replication
stress"
/codon_start=1
/product="tRNA (guanine(9)-N(1))-methyltransferase"
/protein_id="NP_014548.1"
/db_xref="GeneID:854060"
/db_xref="SGD:S000005453"
/translation="MSNDEINQNEEKVKRTPPLPPVPEGMSKKQWKKMCKRQRWEENK
AKYNAERRVKKKRLRHERSAKIQEYIDRGEEVPQELIREPRINVNQTDSGIEIILDCS
FDELMNDKEIVSLSNQVTRAYSANRRANHFAEIKVAPFDKRLKQRFETTLKNTNYENW
NHFKFLPDDKIMFGDEHISKDKIVYLTADTEEKLEKLEPGMRYIVGGIVDKNRYKELC
LKKAQKMGIPTRRLPIDEYINLEGRRVLTTTHVVQLMLKYFDDHNWKNAFESVLPPRK
LDAEAKSASSSPAPKDT"
gene <144204..>145130
/gene="YPQ1"
/locus_tag="YOL092W"
/db_xref="GeneID:854061"
mRNA <144204..>145130
/gene="YPQ1"
/locus_tag="YOL092W"
/product="cationic amino acid transporter"
/transcript_id="NM_001183346.1"
/db_xref="GeneID:854061"
CDS 144204..145130
/gene="YPQ1"
/locus_tag="YOL092W"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane
[PMID:14562095|PMID:22842922|PMID:23169667]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0015174 basic
amino acid transmembrane transporter activity
[PMID:23169667]"
/experiment="EXISTENCE:mutant phenotype:GO:0034488 basic
amino acid transmembrane export from vacuole
[PMID:23169667]"
/note="Putative vacuolar membrane transporter for cationic
amino acids; likely contributes to amino acid homeostasis
by exporting cationic amino acids from the vacuole; member
of the PQ-loop family, with seven transmembrane domains;
similar to mammalian PQLC2 vacuolar transporter; YPQ1 has
a paralog, RTC2, that arose from the whole genome
duplication"
/codon_start=1
/product="cationic amino acid transporter"
/protein_id="NP_014549.1"
/db_xref="GeneID:854061"
/db_xref="SGD:S000005452"
/translation="MQLVPLELNRSTLSGISGSISISCWIIVFVPQIYENFYRKSSDG
LSLLFVVLWLAGDVFNLMGAVMQHLLSTMIILAAYYTVADIILLGQCLWYDNEEKPAV
DPIHLSPANPINENVLHDVFNEQQPLLNSQGQPNRIDEEMAAPSSDGNAGDDNLREVN
SRNLIKDIFIVSGVVFVGFISWYVTYCVNYTQPPPVEDPSLPVPELQINWMAQIFGYL
SALLYLGSRIPQILLNFKRKSCEGISFLFFLFACLGNTTFIFSVIVISLDWKYLIMNA
SWLVGSIGTLFMDFVIFSQFFIYKRNKKFILN"
gene <145334..>147163
/gene="SPO21"
/locus_tag="YOL091W"
/gene_synonym="MPC70"
/db_xref="GeneID:854062"
mRNA <145334..>147163
/gene="SPO21"
/locus_tag="YOL091W"
/gene_synonym="MPC70"
/product="Spo21p"
/transcript_id="NM_001183345.1"
/db_xref="GeneID:854062"
CDS 145334..147163
/gene="SPO21"
/locus_tag="YOL091W"
/gene_synonym="MPC70"
/experiment="EXISTENCE:direct assay:GO:0005198 structural
molecule activity [PMID:12796288]"
/experiment="EXISTENCE:direct assay:GO:0005816 spindle
pole body [PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0035974 meiotic
spindle pole body [PMID:10899120]"
/experiment="EXISTENCE:genetic interaction:GO:0032120
ascospore-type prospore membrane formation
[PMID:10899120]"
/experiment="EXISTENCE:mutant phenotype:GO:0005198
structural molecule activity [PMID:12796288]"
/experiment="EXISTENCE:mutant phenotype:GO:0032120
ascospore-type prospore membrane formation
[PMID:10899120]"
/note="Component of the meiotic outer plaque of the
spindle pole body; involved in modifying the meiotic outer
plaque that is required prior to prospore membrane
formation; SPO21 has a paralog, YSW1, that arose from the
whole genome duplication"
/codon_start=1
/product="Spo21p"
/protein_id="NP_014550.1"
/db_xref="GeneID:854062"
/db_xref="SGD:S000005451"
/translation="MDNILKASNMEGTSTMTVTSRSSEDSSCISNHEQDTDTHKDGDT
SGLENSKISKRKWMKEFFKLSKSPASKSSRSIGSMKSNQSLVSMKSSDDGNSYKNDYS
SICGNSLPSAGLSRSNSVKELKLDSTGSQRSKNNVAMLARSSTTSQTTCSSSSSSSSY
NSIKGNENDILLQNNNHFRHNKEIPQSKGSSNINTASIMSQYNVDTQATAIMSDMQKQ
YDSQQMTSPFVNEDLHFDPNGEVSHVIKAIFKEIGYKYDDFSDIPVFQLMQEMYQLVK
KNSSARRTKITDYASKLKEKEAQLKSQNDKILKLETTNKAYKTKYKEVSLENKKIKEA
FKELDNESYNHDEELLKKYKYTRETLDRVNREQQLIIDQNEFLKKSVNELQNEVNATN
FKFSLFKEKYAKLADSITELNTSTKKREALGENLTFECNELKEICLKYKKNIENISNT
NKNLQNSFKNERKKVLDLRNERNLLKKEILLIECHGSYSLLLVSNILTCYRFLLPSDT
IIETESLIKELLNMNNSLSNHVSSSDEPPAEYSKRLELKCVEFEEKLLYFYQELVTKK
IIDVIYKCFINYYKKSRQTDQKSNQNSSTPYKQSQRQVPHSIK"
gene <147382..>150276
/gene="MSH2"
/locus_tag="YOL090W"
/gene_synonym="PMS5"
/db_xref="GeneID:854063"
mRNA <147382..>150276
/gene="MSH2"
/locus_tag="YOL090W"
/gene_synonym="PMS5"
/product="mismatch repair ATPase MSH2"
/transcript_id="NM_001183344.1"
/db_xref="GeneID:854063"
CDS 147382..150276
/gene="MSH2"
/locus_tag="YOL090W"
/gene_synonym="PMS5"
/experiment="EXISTENCE:direct assay:GO:0000228 nuclear
chromosome [PMID:10882115]"
/experiment="EXISTENCE:direct assay:GO:0000400 four-way
junction DNA binding [PMID:9018043|PMID:10066781]"
/experiment="EXISTENCE:direct assay:GO:0000403 Y-form DNA
binding [PMID:16781730]"
/experiment="EXISTENCE:direct assay:GO:0000404
heteroduplex DNA loop binding [PMID:16781730]"
/experiment="EXISTENCE:direct assay:GO:0000406
double-strand/single-strand DNA junction binding
[PMID:16781730]"
/experiment="EXISTENCE:direct assay:GO:0005524 ATP binding
[PMID:9545323]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:9819445]"
/experiment="EXISTENCE:direct assay:GO:0032135 DNA
insertion or deletion binding [PMID:8805366]"
/experiment="EXISTENCE:direct assay:GO:0032137
guanine/thymine mispair binding [PMID:8816473]"
/experiment="EXISTENCE:direct assay:GO:0032138 single base
insertion or deletion binding [PMID:8816473]"
/experiment="EXISTENCE:genetic interaction:GO:0000735
removal of nonhomologous ends [PMID:9256462]"
/experiment="EXISTENCE:genetic interaction:GO:0030466
silent mating-type cassette heterochromatin formation
[PMID:16157874]"
/experiment="EXISTENCE:genetic interaction:GO:0036297
interstrand cross-link repair [PMID:22912599]"
/experiment="EXISTENCE:mutant phenotype:GO:0000710 meiotic
mismatch repair [PMID:8056309|PMID:16702432|PMID:1334021]"
/experiment="EXISTENCE:mutant phenotype:GO:0000735 removal
of nonhomologous ends [PMID:9256462|PMID:10523644]"
/experiment="EXISTENCE:mutant phenotype:GO:0006298
mismatch repair [PMID:9520271|PMID:10523644]"
/experiment="EXISTENCE:mutant phenotype:GO:0006310 DNA
recombination [PMID:15920474]"
/experiment="EXISTENCE:mutant phenotype:GO:0006311 meiotic
gene conversion [PMID:1334021]"
/experiment="EXISTENCE:mutant phenotype:GO:0006312 mitotic
recombination [PMID:8849883]"
/experiment="EXISTENCE:mutant phenotype:GO:0032138 single
base insertion or deletion binding [PMID:9111312]"
/experiment="EXISTENCE:mutant phenotype:GO:0043111
replication fork arrest [PMID:27045900]"
/experiment="EXISTENCE:physical interaction:GO:0032301
MutSalpha complex [PMID:8816473]"
/experiment="EXISTENCE:physical interaction:GO:0032302
MutSbeta complex [PMID:8805366]"
/note="Protein that binds to DNA mismatches; forms
heterodimers with Msh3p and Msh6p that bind to DNA
mismatches to initiate the mismatch repair process;
contains a Walker ATP-binding motif required for repair
activity and involved in interstrand cross-link repair;
Msh2p-Msh6p binds to and hydrolyzes ATP; required for
silencing at the silent mating-type loci and telomeres"
/codon_start=1
/product="mismatch repair ATPase MSH2"
/protein_id="NP_014551.1"
/db_xref="GeneID:854063"
/db_xref="SGD:S000005450"
/translation="MSSTRPELKFSDVSEERNFYKKYTGLPKKPLKTIRLVDKGDYYT
VIGSDAIFVADSVYHTQSVLKNCQLDPVTAKNFHEPTKYVTVSLQVLATLLKLCLLDL
GYKVEIYDKGWKLIKSASPGNIEQVNELMNMNIDSSIIIASLKVQWNSQDGNCIIGVA
FIDTTAYKVGMLDIVDNEVYSNLESFLIQLGVKECLVQDLTSNSNSNAEMQKVINVID
RCGCVVTLLKNSEFSEKDVELDLTKLLGDDLALSLPQKYSKLSMGACNALIGYLQLLS
EQDQVGKYELVEHKLKEFMKLDASAIKALNLFPQGPQNPFGSNNLAVSGFTSAGNSGK
VTSLFQLLNHCKTNAGVRLLNEWLKQPLTNIDEINKRHDLVDYLIDQIELRQMLTSEY
LPMIPDIRRLTKKLNKRGNLEDVLKIYQFSKRIPEIVQVFTSFLEDDSPTEPVNELVR
SVWLAPLSHHVEPLSKFEEMVETTVDLDAYEENNEFMIKVEFNEELGKIRSKLDTLRD
EIHSIHLDSAEDLGFDPDKKLKLENHHLHGWCMRLTRNDAKELRKHKKYIELSTVKAG
IFFSTKQLKSIANETNILQKEYDKQQSALVREIINITLTYTPVFEKLSLVLAHLDVIA
SFAHTSSYAPIPYIRPKLHPMDSERRTHLISSRHPVLEMQDDISFISNDVTLESGKGD
FLIITGPNMGGKSTYIRQVGVISLMAQIGCFVPCEEAEIAIVDAILCRVGAGDSQLKG
VSTFMVEILETASILKNASKNSLIIVDELGRGTSTYDGFGLAWAIAEHIASKIGCFAL
FATHFHELTELSEKLPNVKNMHVVAHIEKNLKEQKHDDEDITLLYKVEPGISDQSFGI
HVAEVVQFPEKIVKMAKRKANELDDLKTNNEDLKKAKLSLQEVNEGNIRLKALLKEWI
RKVKEEGLHDPSKITEEASQHKIQELLRAIANEPEKENDNYLKYIKALLL"
gene complement(<150398..>153490)
/gene="HAL9"
/locus_tag="YOL089C"
/db_xref="GeneID:854064"
mRNA complement(<150398..>153490)
/gene="HAL9"
/locus_tag="YOL089C"
/product="Hal9p"
/transcript_id="NM_001183343.1"
/db_xref="GeneID:854064"
CDS complement(150398..153490)
/gene="HAL9"
/locus_tag="YOL089C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:19158363]"
/experiment="EXISTENCE:mutant phenotype:GO:0009651
response to salt stress [PMID:9559673]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:11943786|PMID:9559673]"
/note="Transcription factor containing a zinc finger;
overexpression increases salt tolerance through increased
expression of the ENA1 (Na+/Li+ extrusion pump) gene while
gene disruption decreases both salt tolerance and ENA1
expression; HAL9 has a paralog, TBS1, that arose from the
whole genome duplication"
/codon_start=1
/product="Hal9p"
/protein_id="NP_014552.1"
/db_xref="GeneID:854064"
/db_xref="SGD:S000005449"
/translation="MENQGGDYSPNGFSNSASNMNAVFNNEITGRSDISNVNHQTGTP
RLVPETQIWSMPVPDQLMTMPNRENTLMTGSTIGPNIPMNVAYPNTIYSPTEHQSQFQ
TQQNRDISTMMEHTNSNDMSGSGKNLKKRVSKACDHCRKRKIRCDEVDQQTKKCSNCI
KFQLPCTFKHRDEILKKKRKLEIKHHATPGESLQTSNSISNPVASSSVPNSGRFELLN
GNSPLESNIIDKVSNIQNNLNKKMNSKIEKLDRKMSYIIDSVARLEWLLDKAVKKQEG
KYKEKNNLPKPARKIYSTALLTAQKLYWFKQSLGVKASNEEFLSPISEILSISLKWYA
TQMKKFMDLSSPAFFSSEIILYSLPPKKQAKRLLENFHATLLSSVTGIISLKECLDLA
EKYYSESGEKLTYPEHLLLNVCLCSGASATQSIIRGDSKFLRKDRYDPTSQELKKIEN
VALLNAMYYYHKLSTICSGTRTLQALLLLNRYFQLTYDTELANCILGTAIRLAVDMEL
NRKSSYKSLDFEEAIRRRRMWWHCFCTDKLYSLMLSRPPIVGERDMDMLTDQNYYEVI
KTNILPDLIDKKEDLDKITDVNSALNVVVNFCQHISLFISYYVSKLVSIESKIYSTCF
AVRSTLDLSFDAMLDKIKDLNDSLNNWRDNLHVSMKLKSYKQYLSVLYAQKSQENPAL
SFEIACSRVLNCHFRALYSKVILSMMTTSLLIDNERLYKGSRHDIPQLFILFSSQYLN
ASKEMLQLFQGINYQAHMYNEVMYQFSTAMFVLFFYVVDNMNDLKKKGEVKEIIDILK
KSYDRLVGENDEQLLFDNVKWNTLIVFYSHFLKYVLQRYHALNDSTSIFDSKPYDETI
TKVIMHSRKIKDETVDQLIMSLKSYGSLHSLQKGNEADLADDGLNTNDISSEDFAEEA
PINLFGELSVEILKLLKSHSPISNFGDLSPSSNRKGISDDSSLYPIRSDLTSLVYPIH
SSDTGDTLSSGLETPENSNFNSDSGIKEDFEAFRALLPLGKLIYDRDYSFVNTFRDYE
"
gene complement(<153912..>154745)
/gene="MPD2"
/locus_tag="YOL088C"
/db_xref="GeneID:854065"
mRNA complement(<153912..>154745)
/gene="MPD2"
/locus_tag="YOL088C"
/product="protein disulfide isomerase MPD2"
/transcript_id="NM_001183342.1"
/db_xref="GeneID:854065"
CDS complement(153912..154745)
/gene="MPD2"
/locus_tag="YOL088C"
/EC_number="5.3.4.1"
/experiment="EXISTENCE:direct assay:GO:0003756 protein
disulfide isomerase activity [PMID:16002399]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0015035
protein-disulfide reductase activity [PMID:16002399]"
/experiment="EXISTENCE:genetic interaction:GO:0003756
protein disulfide isomerase activity [PMID:11157982]"
/experiment="EXISTENCE:genetic interaction:GO:0005783
endoplasmic reticulum [PMID:15377672]"
/experiment="EXISTENCE:genetic interaction:GO:0006457
protein folding [PMID:11157982]"
/experiment="EXISTENCE:genetic interaction:GO:0015035
protein-disulfide reductase activity [PMID:11157982]"
/experiment="EXISTENCE:mutant phenotype:GO:0003756 protein
disulfide isomerase activity [PMID:11157982]"
/note="Member of the protein disulfide isomerase (PDI)
family; exhibits chaperone activity; overexpression
suppresses the lethality of a pdi1 deletion but does not
complement all Pdi1p functions; undergoes oxidation by
Ero1p"
/codon_start=1
/product="protein disulfide isomerase MPD2"
/protein_id="NP_014553.1"
/db_xref="GeneID:854065"
/db_xref="SGD:S000005448"
/translation="MKLHGFLFSVLSTCVVILPALAYSEAVTMVKSIEQYFDICNRND
SYTMIKYYTSWCQHCKTLAPVYEELGELYAKKANKDDTPINFLEVNCEFFGPTLCTDL
PGFPIIELVKPRTKPLVLPKLDWSSMKFHERLWQRIKTWFNNPKYQLDTSRVVRFEGS
RNLKSLSNFIDTVRSKDTEERFIEHIFDDSRNCNEELRSQQLLCKAGKEYYSDTLSKL
YGDVNGLEKERRRLEALIKQNGDDLSKEVKEKLKIIRLQLSLLSHIEDQLEDTSSHDE
L"
gene complement(<155287..>158637)
/gene="DUF1"
/locus_tag="YOL087C"
/db_xref="GeneID:854066"
mRNA complement(<155287..>158637)
/gene="DUF1"
/locus_tag="YOL087C"
/product="Duf1p"
/transcript_id="NM_001183341.1"
/db_xref="GeneID:854066"
CDS complement(155287..158637)
/gene="DUF1"
/locus_tag="YOL087C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0043130 ubiquitin
binding [PMID:21070969]"
/note="Ubiquitin-binding hypothetical protein; contains
one WD40 repeat in a beta-propeller fold; green
fluorescent protein (GFP)-fusion protein localizes to the
cytoplasm; homolog of human WDR48/UAF1, which is involved
in regulating the Fanconi anemia pathway; deletion mutant
is sensitive to various chemicals including
phenanthroline, sanguinarine, and nordihydroguaiaretic
acid"
/codon_start=1
/product="Duf1p"
/protein_id="NP_014554.1"
/db_xref="GeneID:854066"
/db_xref="SGD:S000005447"
/translation="MNQLTVSYGLISPDYCTSQDAHILPITKILYPDIPGKNYFLTSG
RDGSIILHKNTQLSNEPETAATTIKNDAIRMQVHSDWASDLIHVNMKNSDPSAGDTFI
SVSHDFSIVLISVNAQLTTWDKKIIGDHDDYIKCIVPIHYEMSNDYELEEQEGGPDNV
HDGINNGIVVDEQNNFLFVTGGLDRKIKLWCLSSGPEKMATLLHTFDNAQSNDTGSIY
SMSPIIPKYSFDDNQTSRPFDFVAGDCNGDLIFYSCKYRKEVIRIQNAHRTNIKVVRT
LDDSTRLISTSSDGVINVWDLNCRHDQTTGALQLPKKIGSWSWDSSIWCVQGTSLDKL
YFGDSQGNVMRANLSSYEDAKLTRIFKPDHHHHHHHHHEHEEQNISTTDAKVKKYGGI
LDIALLPNEKLLFSFCTDSNLNVLDLTNNHFSVNEGGFALTRSSLLTNRRHVITENTK
GQMQRWDIVSCELLNTFDSSEGSFDDIVMKYTSKEILSHWCTVSVKVGMLFVKINPKF
LKTEVYGSALKDYQVVNNIEINSDERYNLGKIVINSLFNEFISYEVQKDKLLRKKIFS
LKKKDLTNSLTLDTGYNSESKKNNKDKKRKSTFKISSTLSIGNTNSSGTPPNSAPATP
VMAETIVLEEQPLLQSASDKAIDDSLELVQPLPASKKPYFRTQSSGSLLSRKFKSFRS
TSGRATTGLNTPEEPKGILPDTPHVINDDSAFPQAINTTQQSKDATPESMLWNHPFKL
EQKLSAISSQDLPSNNTHNKLRSSENSRANSTSTLEGNEKKKPEFMPDLLEQIQESYK
QQYMNTSSLKYLTKRLPVTKIIKASSCPIIRVKSATLVLVHLWKEGSCGGRVLFSTLL
PPSHVDNETVSGGKENSKPPDDEEVDLQAVDDDKLGKYDLIDGELGSRLNRRQIFEQL
EENLPYWFAKALFRDIKTVEEQPKLNFLIMPWSSVGGSEAAGNENKKKFISASDTTES
SGNDSSDSSLGNGNEAVSPSTQQQFHNMLKFGRPKTSEQELNPTDLPRISEANVKLVA
PGMIRVKKIKLYVADRFETKTPEMKAKMEPSLWLDLLCRGQVLDNDMTLNTVRTLYWK
SQGDIVLEYRRKVHNSPLVHEVNGNEGK"
gene <159173..>159445
/gene="MHF1"
/locus_tag="YOL086W-A"
/db_xref="GeneID:854067"
mRNA <159173..>159445
/gene="MHF1"
/locus_tag="YOL086W-A"
/product="Mhf1p"
/transcript_id="NM_001184492.1"
/db_xref="GeneID:854067"
CDS 159173..159445
/gene="MHF1"
/locus_tag="YOL086W-A"
/experiment="EXISTENCE:direct assay:GO:0071821 FANCM-MHF
complex [PMID:22325783]"
/experiment="EXISTENCE:genetic interaction:GO:0006974 DNA
damage response [PMID:20347428]"
/note="Component of the heterotetrameric MHF histone-fold
complex; in humans the MHF complex interacts with both DNA
and Mph1p ortholog FANCM, a Fanconi anemia complementation
group protein, to stabilize and remodel blocked
replication forks and repair damaged DNA; mhf1 srs2 double
mutants are MMS hypersensitive; ortholog of human
centromere constitutive-associated network (CCAN) subunit
CENP-S, also known as MHF1"
/codon_start=1
/product="Mhf1p"
/protein_id="NP_076910.1"
/db_xref="GeneID:854067"
/db_xref="SGD:S000007626"
/translation="MNDDEDRAQLKARLWIRVEERLQQVLSSEDIKYTPRFINSLLEL
AYLQLGEMGSDLQAFARHAGRGVVNKSDLMLYLRKQPDLQERVTQE"
gene complement(<159548..>160594)
/gene="ADH1"
/locus_tag="YOL086C"
/gene_synonym="ADC1"
/db_xref="GeneID:854068"
mRNA complement(<159548..>160594)
/gene="ADH1"
/locus_tag="YOL086C"
/gene_synonym="ADC1"
/product="alcohol dehydrogenase ADH1"
/transcript_id="NM_001183340.1"
/db_xref="GeneID:854068"
CDS complement(159548..160594)
/gene="ADH1"
/locus_tag="YOL086C"
/gene_synonym="ADC1"
/EC_number="1.1.1.1"
/EC_number="1.1.1.54"
/EC_number="1.1.1.78"
/experiment="EXISTENCE:direct assay:GO:0004022 alcohol
dehydrogenase (NAD+) activity [PMID:6985717]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:2937632]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836]"
/experiment="EXISTENCE:direct assay:GO:0019170
methylglyoxal reductase (NADH) activity [PMID:12722185]"
/experiment="EXISTENCE:direct assay:GO:1904408 melatonin
binding [PMID:31708896]"
/experiment="EXISTENCE:genetic interaction:GO:0000947
amino acid catabolic process to alcohol via Ehrlich
pathway [PMID:12499363]"
/experiment="EXISTENCE:mutant phenotype:GO:0019655
pyruvate fermentation to ethanol [PMID:15082781]"
/note="Alcohol dehydrogenase; fermentative isozyme active
as homo- or heterotetramers; required for the reduction of
acetaldehyde to ethanol, the last step in the glycolytic
pathway; ADH1 has a paralog, ADH5, that arose from the
whole genome duplication"
/codon_start=1
/product="alcohol dehydrogenase ADH1"
/protein_id="NP_014555.1"
/db_xref="GeneID:854068"
/db_xref="SGD:S000005446"
/translation="MSIPETQKGVIFYESHGKLEYKDIPVPKPKANELLINVKYSGVC
HTDLHAWHGDWPLPVKLPLVGGHEGAGVVVGMGENVKGWKIGDYAGIKWLNGSCMACE
YCELGNESNCPHADLSGYTHDGSFQQYATADAVQAAHIPQGTDLAQVAPILCAGITVY
KALKSANLMAGHWVAISGAAGGLGSLAVQYAKAMGYRVLGIDGGEGKEELFRSIGGEV
FIDFTKEKDIVGAVLKATDGGAHGVINVSVSEAAIEASTRYVRANGTTVLVGMPAGAK
CCSDVFNQVVKSISIVGSYVGNRADTREALDFFARGLVKSPIKVVGLSTLPEIYEKME
KGQIVGRYVVDTSK"
gene complement(<161673..>162014)
/locus_tag="YOL085C"
/db_xref="GeneID:854069"
mRNA complement(<161673..>162014)
/locus_tag="YOL085C"
/product="uncharacterized protein"
/transcript_id="NM_001348875.1"
/db_xref="GeneID:854069"
CDS complement(161673..162014)
/locus_tag="YOL085C"
/note="hypothetical protein; conserved among S. cerevisiae
strains; YOL085C is not an essential gene; partially
overlaps dubious ORF YOL085W-A"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335813.1"
/db_xref="GeneID:854069"
/db_xref="SGD:S000005445"
/translation="MKAKDKYKGRTKNKVKSVDMMYLALRRRKNEFTQLHNHADSVAD
PRSCRPGDNAGREAVPGGVFCACIFQGLPCAKGRDWRSNKNAGWGCDDDDHDNWCHYL
SCRKNLSAFAT"
gene <162356..>165331
/gene="PHM7"
/locus_tag="YOL084W"
/db_xref="GeneID:854070"
mRNA <162356..>165331
/gene="PHM7"
/locus_tag="YOL084W"
/product="Phm7p"
/transcript_id="NM_001183338.1"
/db_xref="GeneID:854070"
CDS 162356..165331
/gene="PHM7"
/locus_tag="YOL084W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0006817
phosphate ion transport [PMID:36592238]"
/note="hypothetical protein; expression is regulated by
phosphate levels; green fluorescent protein (GFP)-fusion
protein localizes to the cell periphery and vacuole;
protein abundance increases in response to DNA replication
stress"
/codon_start=1
/product="Phm7p"
/protein_id="NP_014557.1"
/db_xref="GeneID:854070"
/db_xref="SGD:S000005444"
/translation="MADSSSTSAFISTLIIYGLTAVVFVWLFLLLRPKNRRVYEPRSL
KDIQTIPEEERTEPVPEGYFGWVEYLLSKPHSFLIQHTSVDGYFLLRYIGIVGSLSFV
GCLLLLPILLPVNATNGNNLQGFELLSFSNVTNKNRFYAHVFLSWIFFGLFTYVIYKE
LYYYVVFRHAMQTTPLYDGLLSSRTVIVTELHKSIAQEGEMQMRFPKASNVAFAYDLS
DLQELCKERAKNAAKYEAALNKVLNKCVKMTRNKTQKQLDKLYNNGTKPKDDLETYVP
HKKRPKHRLGKLPLCLGGKKVNTLSYSSKRIGELNEEIHEKQADWASNDRQPACFIQF
ETQLEAQRCYQSVEAILGKKNFGKRLIGYSPEDVNWGSMRLSSKERHSRRAVANTIMV
LLIIFWAFPVAVVGIISNVNFLTDKVPFLRFINNMPTFLMGVITGLLPTIALVVLMSL
VPPFIVMLGKLSGCVTRQETDLYSQAWYYAFAVIQIFLVVTATSSASSTVDSIIDRPR
SAMTLLANNLPKASNFYIMYFILKGLTGPTWTILQAVNLLLSKVLGRVLDSTPRQKWN
RYNTLATPRMGIVYPGIEILVCIYICYSIIAPILLFFSTVMLTLLYVAYLYNLNYVFG
FSFDLKGRNYPRALFQIFVGIYLSEVCLLGLFIMAKTWGPLVLEVFWIVVTALAHIYM
KRKFIPLFDAVPLSAIRHARGEPGYSYPTSDLGLQEIKDIADEMKGKYEQDNTHGILT
PVTKDDLKKANLIPDNDGSSENGTPSNPFESGSERASLSGSNAESDSIKKLNDTVIKK
SSTLSSSTKDNNESTFVPEGEKFRKFHYSDVEALRNKRPYDEDDHSKHGPEGAVPVNA
DAGVIYSDPAAVMKEPQAFPPDVLETNTWTRRILQFFNPRRSYPFDSVRMRFPLVFNT
SIEYDEEYLSSAYTDPCVREKDPIVWCCKDPLGVSKQQIQEARSNGLDVRDDFTRYDE
KGKVIFTYNPPDYEPEAKK"
gene <165714..>166952
/gene="ATG34"
/locus_tag="YOL083W"
/db_xref="GeneID:854071"
mRNA <165714..>166952
/gene="ATG34"
/locus_tag="YOL083W"
/product="Atg34p"
/transcript_id="NM_001183337.1"
/db_xref="GeneID:854071"
CDS 165714..166952
/gene="ATG34"
/locus_tag="YOL083W"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0034270 Cvt complex
[PMID:20659891|PMID:20639194]"
/experiment="EXISTENCE:genetic interaction:GO:0016236
macroautophagy [PMID:20639194]"
/experiment="EXISTENCE:genetic interaction:GO:0030674
protein-macromolecule adaptor activity [PMID:20639194]"
/experiment="EXISTENCE:genetic interaction:GO:0031503
protein-containing complex localization
[PMID:20659891|PMID:20639194]"
/experiment="EXISTENCE:genetic interaction:GO:0071211
protein targeting to vacuole involved in autophagy
[PMID:20639194]"
/experiment="EXISTENCE:mutant phenotype:GO:0065003
protein-containing complex assembly [PMID:20639194]"
/note="Receptor protein involved in selective autophagy
during starvation; specifically involved in the transport
of cargo protein alpha-mannosidase (Ams1p); Atg19p
paralog"
/codon_start=1
/product="Atg34p"
/protein_id="NP_014558.1"
/db_xref="GeneID:854071"
/db_xref="SGD:S000005443"
/translation="MKIAVETTLFDFFVIDQFKKSTFSAPNTKVDTIKGCINKFIEQF
NVYDEQHIFWQPPGKSNVRLLSNANDFGQLGNFLHKKIKCNIFIGEEALRKYDLNICG
PYDKFVENSDPSVKKVVNRDDVMLSRKCLNIISEQLSILEKSISKAQNQVLQSSEVEG
KKCIILPEDKPELIKFFSKFETSVQLQEVYEGYKVYEKLLQKFGGQKKRMESFLNENT
PMSGAEAIKQINISEELKEKGERLTTPNDPLLHVEVSNEDNSLHFILYNKTNIIIPGN
CTFEFSSQISEVFSIKMGPHEIGIKGQKELWFFPSLPTPLSNYTMKVVNQDGETILVG
KCADSNEITLKSPLASFSTGSFQTGSFHTLQDPTNVFRADALSSPDESSIMSTPFLGE
TDEVYNSGSTLSRPFTWEEI"
rep_origin 166976..167221
/note="ARS1510; Autonomously Replicating Sequence"
/db_xref="SGD:S000118487"
regulatory 168632..168679
/regulatory_class="other"
/note="Upstream open reading frame (uORF) in 5'
untranslated region of ATG19 gene, regulate translation"
gene <168727..>169974
/gene="ATG19"
/locus_tag="YOL082W"
/gene_synonym="CVT19"
/db_xref="GeneID:854072"
mRNA <168727..>169974
/gene="ATG19"
/locus_tag="YOL082W"
/gene_synonym="CVT19"
/product="Atg19p"
/transcript_id="NM_001183336.1"
/db_xref="GeneID:854072"
CDS 168727..169974
/gene="ATG19"
/locus_tag="YOL082W"
/gene_synonym="CVT19"
/experiment="EXISTENCE:direct assay:GO:0000407 phagophore
assembly site [PMID:18497569|PMID:12479808]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11382752]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0034270 Cvt complex
[PMID:11430817|PMID:12479808|PMID:20659891]"
/experiment="EXISTENCE:genetic interaction:GO:0031503
protein-containing complex localization [PMID:20659891]"
/experiment="EXISTENCE:mutant phenotype:GO:0016050 vesicle
organization [PMID:15138258]"
/experiment="EXISTENCE:mutant phenotype:GO:0030674
protein-macromolecule adaptor activity [PMID:12479808]"
/experiment="EXISTENCE:mutant phenotype:GO:0031503
protein-containing complex localization
[PMID:12479808|PMID:12479807|PMID:20659891|PMID:11430817]"
/experiment="EXISTENCE:mutant phenotype:GO:0036503 ERAD
pathway [PMID:17238920]"
/note="Receptor protein for the cytoplasm-to-vacuole
targeting (Cvt) pathway; delivers cargo proteins
aminopeptidase I (Ape1p) and alpha-mannosidase (Ams1p) to
the phagophore assembly site for packaging into Cvt
vesicles; interaction with Atg19p during the Cvt pathway
requires phosphorylation by Hrr25p"
/codon_start=1
/product="Atg19p"
/protein_id="NP_014559.1"
/db_xref="GeneID:854072"
/db_xref="SGD:S000005442"
/translation="MNNSKTNQQMNTSMGYPLTVYDECNKFQLIVPTLDANIMLWCIG
QLSLLNDSNGCKHLFWQPNDKSNVRILLNNYDYGHLFKYLQCQRKCSVYIGEGTLKKY
NLTISTSFDNFLDLTPSEEKESLCREDAHEDPVSPKAGSEEEISPNSTSNVVVSRECL
DNFMKQLLKLEESLNKLELEQKVTNKEPNHRISGTIDIPEDRSELVNFFTELKTVKQL
EDVFQRYHDYERLSQECDSKTEIASDHSKKETKIEVEPPNERSLQITMNQRDNSLYFQ
LFNNTNSVLAGNCKLKFTDAGDKPTTQIIDMGPHEIGIKEYKEYRYFPYALDLEAGST
IEIENQYGEVIFLGKYGSSPMINLRPPSRLSAESLQASQEPFYSFQIDTLPELDDSSI
ISTSISLSYDGDDNEKALTWEEL"
gene <171070..>180309
/gene="IRA2"
/locus_tag="YOL081W"
/gene_synonym="CCS1; GLC4"
/db_xref="GeneID:854073"
mRNA <171070..>180309
/gene="IRA2"
/locus_tag="YOL081W"
/gene_synonym="CCS1; GLC4"
/product="Ras GTPase activating protein IRA2"
/transcript_id="NM_001183335.1"
/db_xref="GeneID:854073"
CDS 171070..180309
/gene="IRA2"
/locus_tag="YOL081W"
/gene_synonym="CCS1; GLC4"
/experiment="EXISTENCE:direct assay:GO:0005096 GTPase
activator activity [PMID:7577970]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:22575457|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:22575457]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0005096 GTPase
activator activity [PMID:2178777]"
/experiment="EXISTENCE:mutant phenotype:GO:0007193
adenylate cyclase-inhibiting G protein-coupled receptor
signaling pathway [PMID:2164637|PMID:15629464]"
/experiment="EXISTENCE:mutant phenotype:GO:0046580
negative regulation of Ras protein signal transduction
[PMID:15339905]"
/note="GTPase-activating protein; negatively regulates RAS
by converting it from the GTP- to the GDP-bound inactive
form, required for reducing cAMP levels under nutrient
limiting conditions; IRA2 has a paralog, IRA1, that arose
from the whole genome duplication; defects in human
homolog NF1 are associated with neurofibromatosis"
/codon_start=1
/product="Ras GTPase activating protein IRA2"
/protein_id="NP_014560.1"
/db_xref="GeneID:854073"
/db_xref="SGD:S000005441"
/translation="MSQPTKNKKKEHGTDSKSSRMTRTLVNHILFERILPILPVESNL
STYSEVEEYSSFISCRSVLINVTVSRDANAMVEGTLELIESLLQGHEIISDKGSSDVI
ESILIILRLLSDALEYNWQNQESLHYNDISTHVEHDQEQKYRPKLNSILPDYSSTHSN
GNKHFFHQSKPQALIPELASKLLESCAKLKFNTRTLQILQNMISHVHGNILTTLSSSI
LPRHKSYLTRHNHPSHCKMIDSTLGHILRFVAASNPSEYFEFIRKSVQVPVTQTHTHS
HSHSHSLPSSVYNSIVPHFDLFSFIYLSKHNFKKYLELIKNLSVTLRKTIYHCLLLHY
SAKAIMFWIMARPAEYYELFNLLKDNNNEHSKSLNTLNHTLFEEIHSTFNVNSMITTN
QNAHQGSSSPSSSSPSSPPSSSSSDNNNQNIIAKSLSRQLSHHQSYIQQQSERKLHSS
WTTNSQSSTSLSSSTSNSTTTDFSTHTQPGEYDPSLPDTPTMSNITISASSLLSQTPT
PTTQLQQRLNSAAAAAAAAASPSNSTPTGYTAEQQSRASYDAHKTGHTGKDYDEHFLS
VTRLDNVLELYTHFDDTEVLPHTSVLKFLTTLTMFDIDLFNELNATSFKYIPDCTMHR
PKERTSSFNNTAHETGSEKTSGIKHITQGLKKLTSLPSSTKKTVKFVKMLLRNLNGNQ
AVSDVALLDTMRALLSFFTMTSAVFLVDRNLPSVLFAKRLIPIMGTNLSVGQDWNSKI
NNSLMVCLKKNSTTFVQLQLIFFSSAIQFDHELLLARLSIDTMANNLNMQKLCLYTEG
FRIFFDIPSKKELRKAIAVKISKFFKTLFSIIADILLQEFPYFDEQITDIVASILDGT
IINEYGTKKHFKGSSPSLCSTTRSRSGSTSQSSMTPVSPLGLDTDICPMNTLSLVGSS
TSRNSDNVNSLNSSPKNLSSDPYLSHLVAPRARHALGGPSSIIRNKIPTTLTSPPGTE
KSSPVQRPQTESISATPMAITNSTPLSSAAFGIRSPLQKIRTRRYSDESLGKFMKSTN
NYIQEHLIPKDLNEATLQDARRIMINIFSIFKRPNSYFIIPHNINSNLQWVSQDFRNI
MKPIFVAIVSPDVDLQNTAQSFMDTLLSNVITYGESDENISIEGYHLLCSYTVTLFAM
GLFDLKINNEKRQILLDITVKFMKVRSHLAGIAEASHHMEYISDSEKLTFPLIMGTVG
RALFVSLYSSQQKIEKTLKIAYTEYLSAINFHERNIDDADKTWVHNIEFVEAMCHDNY
TTSGSIAFQRRTRNNILRFATIPNAILLDSMRMIYKKWHTYTHSKSLEKQERNDFRNF
AGILASLSGILFINKKILQEMYPYLLDTVSELKKNIDSFISKQCQWLNYPDLLTRENS
RDILSVELHPLSFNLLFNNLRLKLKELACSDLSIPENESSYVLLEQIIKMLRTILGRD
DDNYVMMLFSTEIVDLIDLLTDEIKKIPAYCPKYLKAIIQMTKMFSALQHSEVNLGVK
NHFHVKNKWLRQITDWFQVSIAREYDFENLSKPLKEMDLVKRDMDILYIDTAIEASTA
IAYLTRHTFLEIPPAASDPELSRSRSVIFGFYFNILMKGLEKSSDRDNYPVFLRHKMS
VLNDNVILSLTNLSNTNVDASLQFTLPMGYSGNRNIRNAFLEVFINIVTNYRTYTAKT
DLGKLEAADKFLRYTIEHPQLSSFGAAVCPASDIDAYAAGLINAFETRNATHIVVAQL
IKNEIEKSSRPTDILRRNSCATRSLSMLARSKGNEYLIRTLQPLLKKIIQNRDFFEIE
KLKPEDSDAERQIELFVKYMNELLESISNSVSYFPPPLFYICQNIYKVACEKFPDHAI
IAAGSFVFLRFFCPALVSPDSENIIDISHLSEKRTFISLAKVIQNIANGSENFSRWPA
LCSQKDFLKECSDRIFRFLAELCRTDRTIDIQVRTDPTPIAFDYQFLHSFVYLYGLEV
RRNVLNEAKHDDGDIDGDDFYKTTFLLIDDVLGQLGQPKMEFSNEIPIYIREHMDDYP
ELYEFMNRHAFRNIETSTAYSPSVHESTSSEGIPIITLTMSNFSDRHVDIDTVAYKFL
QIYARIWTTKHCLIIDCTEFDEGGLDMRKFISLVMGLLPEVAPKNCIGCYYFNVNETF
MDNYGKCLDKDNVYVSSKIPHYFINSNSDEGLMKSVGITGQGLKVLQDIRVSLHDITL
YDEKRNRFTPVSLKIGDIYFQVLHETPRQYKIRDMGTLFDVKFNDVYEISRIFEVHVS
SITGVAAEFTVTFQDERRLIFSSPKYLEIVKMFYYAQIRLESEYEMDNNSSTSSPNSN
NKDKQQKERTKLLCHLLLVSLIGLFDESKKMKNSSYNLIAATEASFGLNFGSHFHRSP
EVYVPEDTTTFLGVIGKSLAESNPELTAYMFIYVLEALKNNVIPHVYIPHTICGLSYW
IPNLYQHVYLADDEEGPENISHIFRILIRLSVRETDFKAVYMQYVWLLLLDDGRLTDI
IVDEVINHALERDSENRDWKKTISLLTVLPTTEVANNIIQKILAKIRSFLPSLKLEAM
TQSWSELTILVKISIHVFFETSLLVQMYLPEILFIVSLLIDVGPRELRSSLHQLLMNV
CHSLAINSALPQDHRNNLDEISDIFAHQKVKFMFGFSEDKGRILQIFSASSFASKFNI
LDFFINNILLLMEYSSTYEANVWKTRYKKYVLESVFTSNSFLSARSIMIVGIMGKSYI
TEGLCKAMLIETMKVIAEPKITDEHLFLAISHIFTYSKIVEGLDPNLDLMKHLFWFST
LFLESRHPIIFEGALLFVSNCIRRLYMAQFENESETSLISTLLKGRKFAHTFLSKIEN
LSGIVWNEDNFTHILIFIINKGLSNPFIKSTAFDFLKMMFRNSYFEHQINQKSDHYLC
YMFLLYFVLNCNQFEELLGDVDFEGEMVNIENKNTIPKILLEWLSSDNENANITLYQG
AILFKCSVTDEPSRFRFALIIRHLLTKKPICALRFYSVIRNEIRKISAFEQNSDCVPL
AFDILNLLVTHSESNSLEKLHEESIERLTKRGLSIVTSSGIFAKNSDMMIPLDVKPED
IYERKRIMTMILSRMSCSA"
gene complement(<180558..>181427)
/gene="REX4"
/locus_tag="YOL080C"
/db_xref="GeneID:854075"
mRNA complement(<180558..>181427)
/gene="REX4"
/locus_tag="YOL080C"
/product="putative 3'-5' exonuclease"
/transcript_id="NM_001183334.1"
/db_xref="GeneID:854075"
CDS complement(180558..181427)
/gene="REX4"
/locus_tag="YOL080C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0000027
ribosomal large subunit assembly [PMID:12364601]"
/experiment="EXISTENCE:genetic interaction:GO:0006364 rRNA
processing [PMID:12364601]"
/note="Putative RNA exonuclease; possibly involved in
pre-rRNA processing and ribosome assembly"
/codon_start=1
/product="putative 3'-5' exonuclease"
/protein_id="NP_014561.1"
/db_xref="GeneID:854075"
/db_xref="SGD:S000005440"
/translation="MALSSNWQALLASESNPTSNGKNKQSNRKIRNVKKVSKTVNVSS
TTQYAPRKRKNGSKIMDMVYNMNKEISKHEKDKLEGKVFEFNPNKANTSTTIKEPVKV
GISEDTRINSNKSKEIGKYIAMDCEFVGVGPEGKESALARISIVNYFGHVVLDEFVKP
REKVVEWRTWVSGIKPEHMKNAITFKEAQKKTADILEGRILVGHALKHDLEALMLSHP
KSLLRDTSRHLPFRKLYAKGKTPSLKKLTREVLKISIQEGEHSSVEDARATMLLYKKE
KTEFEKIHRNTFN"
gene <181682..>185212
/gene="AVO1"
/locus_tag="YOL078W"
/db_xref="GeneID:854076"
mRNA <181682..>185212
/gene="AVO1"
/locus_tag="YOL078W"
/product="Avo1p"
/transcript_id="NM_001183332.1"
/db_xref="GeneID:854076"
CDS 181682..185212
/gene="AVO1"
/locus_tag="YOL078W"
/experiment="EXISTENCE:direct assay:GO:0005546
phosphatidylinositol-4,5-bisphosphate binding
[PMID:19144819]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:19144819]"
/experiment="EXISTENCE:mutant phenotype:GO:0030950
establishment or maintenance of actin cytoskeleton
polarity [PMID:12408816]"
/experiment="EXISTENCE:mutant phenotype:GO:0060090
molecular adaptor activity [PMID:16002396]"
/experiment="EXISTENCE:physical interaction:GO:0001558
regulation of cell growth [PMID:12408816]"
/experiment="EXISTENCE:physical interaction:GO:0005737
cytoplasm [PMID:12408816]"
/experiment="EXISTENCE:physical interaction:GO:0030950
establishment or maintenance of actin cytoskeleton
polarity [PMID:12408816]"
/experiment="EXISTENCE:physical interaction:GO:0031932
TORC2 complex [PMID:12408816]"
/note="Subunit of TORC2, a regulator of plasma membrane
(PM) homeostasis; C-terminal PH domain binds to
phosphoinositide-(4,5)-bisphosphate (PI(4,5)P2); binds
directly to Ypk2p a Tor2p substrate and downstream TORC2
effector through a conserved central CRIM domain; acts
with Tsc11p as a scaffold important for the integrity of
the TORC2 complex; dispensable for PM localization of
TORC2; role in regulating actin organization and growth
control; orthologous to human MAPKAP1/SIN1"
/codon_start=1
/product="Avo1p"
/protein_id="NP_014563.1"
/db_xref="GeneID:854076"
/db_xref="SGD:S000005438"
/translation="MDTVTVLNELRAQFLRVCPEKDQMKRIIKPYIPVDEFNTEQCLD
SSIRELYMNSDGVSLLPELESPPVSKDFMENYASLGKMRIMRENEGQKGKANQNLIGA
EKTERDEEETRNLQDKSAKNTLIVEENGTLRYNPLNSSASNSLLNDDDHTSGKHHKTS
SKEDSYLNSSMEMQKKSSKRSSLPFVRIFKSRRDHSNTSGNKNVMNTTNTRAKSSTLH
PPGARHNKKGSKFDMNFDFDENLEEEDDDDDDDEEGDDIHSQFFQLDDDFDAKGSGAS
PAHKGINGMSNNKNNTYTNNRNSISILDDRESSNGNIGSASRLKSHFPTSQKGKIFLT
DNKNDGQKSDSLNANKGIHGDGSSASGNGSVSRDGLTETESNNISDMESYINEKDLDD
LNFDTVTSNINKTVSDLGGHESTNDGTAVMNRDSKDSRSNSNEFNAQNRDRITPGSSY
GKSLLGSEYSEERYSNNDSSTMESGEMSLDSDMQTNTIPSHSIPMSMQKYGIYHGDDD
STLNNVFDKAVLTMNSSRHPKERRDTVISGKEPTSLTSSNRKFSVSSNLTSTRSPLLR
GHGRTSSTASSEHMKAPKVSDSVLHRARKSTLTLKQDHSQPSVPSSVHKSSKEGNILI
EKTTDYLVSKPKASQLSNMFNKKKKRTNTNSVDVLEYFSFVCGDKVPNYESMGLEIYI
QASKKYKRNSFTTKVRKSSTIFEVIGFALFLYSTEKKPDNFEEDGLTVEDISNPNNFS
LKIVDEDGEPFEDNFGKLDRKSTIQSISDSEVVLCKVDDAEKSQNEIETPLPFETGGG
LMDASTLDANSSHDTTDGTINQLSFYKPIIGNEDDIDKTNGSKIIDVTVYLYPNVNPK
FNYTTISVLVTSHINDILVKYCKMKNMDPNEYALKVLGKNYILDLNDTVLRLDGINKV
ELISKKDARELHLEKMKPDLKKPVLPTIQSNDLTPLTLEPLNSYLKADAGGAVAAIPE
NTKVTSKAKKISTKYKLGLAKQHSSSSVASGSVSTAGGLANGNGFFKNKNSSKSSLHG
TLQFHNINRSQSTMEHTPDTPNGVGDNFQDLFTGAYHKYKVWRRQQMSFINKHERTLA
IDGDYIYIVPPEGRIHWHDNVKTKSLHISQVVLVKKSKRVPEHFKIFVRREGQDDIKR
YYFEAVSGQECTEIVTRLQNLLSAYRMNHK"
gene <185438..>185644
/gene="ATP19"
/locus_tag="YOL077W-A"
/db_xref="GeneID:854077"
mRNA <185438..>185644
/gene="ATP19"
/locus_tag="YOL077W-A"
/product="F1F0 ATP synthase subunit k"
/transcript_id="NM_001184377.1"
/db_xref="GeneID:854077"
CDS 185438..185644
/gene="ATP19"
/locus_tag="YOL077W-A"
/experiment="EXISTENCE:curator inference:GO:0046933
proton-transporting ATP synthase activity, rotational
mechanism [PMID:9857174]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:mutant phenotype:GO:0005739
mitochondrion [PMID:20219971]"
/experiment="EXISTENCE:mutant phenotype:GO:0015986 proton
motive force-driven ATP synthesis [PMID:9857174]"
/experiment="EXISTENCE:mutant phenotype:GO:0045259
proton-transporting ATP synthase complex [PMID:9857174]"
/experiment="EXISTENCE:mutant phenotype:GO:0065003
protein-containing complex assembly [PMID:20219971]"
/note="Subunit k of the mitochondrial F1F0 ATP synthase;
F1F0 ATP synthase is a large, evolutionarily conserved
enzyme complex required for ATP synthesis; associated only
with the dimeric form of ATP synthase"
/codon_start=1
/product="F1F0 ATP synthase subunit k"
/protein_id="NP_014564.1"
/db_xref="GeneID:854077"
/db_xref="SGD:S000007339"
/translation="MGAAYHFMGKAIPPHQLAIGTLGLLGLLVVPNPFKSAKPKTVDI
KTDNKDEEKFIENYLKKHSEKQDA"
gene complement(<185848..>186723)
/gene="BRX1"
/locus_tag="YOL077C"
/db_xref="GeneID:854078"
mRNA complement(<185848..>186723)
/gene="BRX1"
/locus_tag="YOL077C"
/product="ribosome biogenesis protein BRX1"
/transcript_id="NM_001183331.2"
/db_xref="GeneID:854078"
CDS complement(185848..186723)
/gene="BRX1"
/locus_tag="YOL077C"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:11843177]"
/experiment="EXISTENCE:direct assay:GO:0008097 5S rRNA
binding [PMID:11864606]"
/experiment="EXISTENCE:direct assay:GO:0030687
preribosome, large subunit precursor
[PMID:17443350|PMID:11583614]"
/experiment="EXISTENCE:direct assay:GO:0042134 rRNA
primary transcript binding [PMID:11864606]"
/experiment="EXISTENCE:mutant phenotype:GO:0000027
ribosomal large subunit assembly [PMID:11843177]"
/experiment="EXISTENCE:mutant phenotype:GO:0000464
endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA
from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA,
LSU-rRNA) [PMID:11843177]"
/experiment="EXISTENCE:mutant phenotype:GO:0000465
exonucleolytic trimming to generate mature 5'-end of 5.8S
rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S
rRNA, LSU-rRNA) [PMID:11843177]"
/note="Nucleolar protein; constituent of 66S pre-ribosomal
particles; depletion leads to defects in rRNA processing
and a block in the assembly of large ribosomal subunits;
possesses a sigma(70)-like RNA-binding motif"
/codon_start=1
/product="ribosome biogenesis protein BRX1"
/protein_id="NP_014565.2"
/db_xref="GeneID:854078"
/db_xref="SGD:S000005437"
/translation="MSSIYKALAGKSKDNKSEKKQGNVKQFMNKQRTLLISSRGVNYR
HRHLIQDLSGLLPHSRKEPKLDTKKDLQQLNEIAELYNCNNVLFFEARKHQDLYLWLS
KPPNGPTIKFYIQNLHTMDELNFTGNCLKGSRPVLSFDQRFESSPHYQLIKELLVHNF
GVPPNARKSKPFIDHVMSFSIVDDKIWVRTYEISHSTKNKEEYEDGEEDISLVEIGPR
FVMTVILILEGSFGGPKIYENKQYVSPNVVRAQIKQQAAEEAKSRAEAAVERKIKRRE
NVLAADPLSNDALFK"
gene <187024..>189414
/gene="MDM20"
/locus_tag="YOL076W"
/gene_synonym="DEC1; NAA25"
/db_xref="GeneID:854079"
mRNA <187024..>189414
/gene="MDM20"
/locus_tag="YOL076W"
/gene_synonym="DEC1; NAA25"
/product="Mdm20p"
/transcript_id="NM_001183330.1"
/db_xref="GeneID:854079"
CDS 187024..189414
/gene="MDM20"
/locus_tag="YOL076W"
/gene_synonym="DEC1; NAA25"
/experiment="EXISTENCE:direct assay:GO:0031416 NatB
complex [PMID:12783868]"
/experiment="EXISTENCE:genetic interaction:GO:0000001
mitochondrion inheritance [PMID:9105043]"
/experiment="EXISTENCE:genetic interaction:GO:0017196
N-terminal peptidyl-methionine acetylation
[PMID:12783868]"
/experiment="EXISTENCE:genetic interaction:GO:0032956
regulation of actin cytoskeleton organization
[PMID:12808144]"
/experiment="EXISTENCE:mutant phenotype:GO:0000001
mitochondrion inheritance [PMID:9105043]"
/experiment="EXISTENCE:mutant phenotype:GO:0004596
protein-N-terminal amino-acid acetyltransferase activity
[PMID:12783868]"
/experiment="EXISTENCE:mutant phenotype:GO:0007010
cytoskeleton organization [PMID:9105043]"
/experiment="EXISTENCE:mutant phenotype:GO:0017196
N-terminal peptidyl-methionine acetylation
[PMID:12783868]"
/experiment="EXISTENCE:mutant phenotype:GO:0032956
regulation of actin cytoskeleton organization
[PMID:12808144]"
/note="Non-catalytic subunit of the NatB N-terminal
acetyltransferase; NatB catalyzes N-acetylation of
proteins with specific N-terminal sequences; involved in
mitochondrial inheritance and actin assembly"
/codon_start=1
/product="Mdm20p"
/protein_id="NP_014566.1"
/db_xref="GeneID:854079"
/db_xref="SGD:S000005436"
/translation="MSDKIQEEILGLVSRSNFKQCYAKLGQLQKQFPNALYFKILETY
VKFKQSPGKFDYNKLLEEPYGLKGTTITGDTRSLEFLHNFFVELGKYDEALHVYERGN
FKFPSYELSYHWFMKALEDSNYNQMSKASLQLAKYSDSGNLPKRAYYFWNAISILAVS
RFQENTLSDPKKILLSRLARQSLLDLKPFQNVQEIIVYCLVLDELFPQSREISEEIVA
ITFANFDTSVNLYLKNFILKHTKLLNSPQKLFEVCSKLIEKGLDDYELITNLIDAAYK
LSKSKDEVKQWIDENLGDSRNTRLARLKLDIMYTDSVSESSLSYYLSKYHNKPCCSID
LNHYSGHINIDMLKSIMSKYDPEDKDLIHHCNILELGLIGSDSINNYNKFKGTLEKKS
VTDYSSCSTFLLEIVKDKCKKTNPELKDVLLCITILENYQAKDPHNFDTMCWLIVLYM
YLGLVPDAYFHFINLKIKNVQTDSLDYMIFSRFSTLFPNKQSDFYSKTFHEHNNLYDT
SLANLPRYIQVAFERNSYSKILGMLEMRDKLMKSYTRWTKTLENLQFSRLCNDKRGHL
LQKLHEDWRSLEMTQSVSFSDNRDFSILDENFAQFLNRGKILEYANLNEESIFLTLIR
ELIIEALPNGEKTEQISALLKKLPSINLEELLNNNLTEVESASFLIFFEIYENNGKNL
HDLISRLMKVPINAKQNWMVSHTYLTKMATLKTLDSLKRIKDKEIQKLIKNSLKELRS
CCDDVFKGYSKALVQAYEELKKDECGNLLKELDVKAENVKNIKNSLLGIQKSVRNL"
gene complement(<189658..>193542)
/locus_tag="YOL075C"
/db_xref="GeneID:854080"
mRNA complement(<189658..>193542)
/locus_tag="YOL075C"
/product="uncharacterized protein"
/transcript_id="NM_001183329.2"
/db_xref="GeneID:854080"
CDS complement(189658..193542)
/locus_tag="YOL075C"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:23658021]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="Putative ABC transporter"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014567.2"
/db_xref="GeneID:854080"
/db_xref="SGD:S000005435"
/translation="MSQQENGDVATELIENRLSFSRIPRISLHVRDLSIVASKTNTTL
VNTFSMDLPSGSVMAVMGGSGSGKTTLLNVLASKISGGLTHNGSIRYVLEDTGSEPNE
TEPKRAHLDGQDHPIQKHVIMAYLPQQDVLSPRLTCRETLKFAADLKLNSSERTKKLM
VEQLIEELGLKDCADTLVGDNSHRGLSGGEKRRLSIGTQMISNPSIMFLDEPTTGLDA
YSAFLVIKTLKKLAKEDGRTFIMSIHQPRSDILFLLDQVCILSKGNVVYCDKMDNTIP
YFESIGYHVPQLVNPADYFIDLSSVDSRSDKEEAATQSRLNSLIDHWHDYERTHLQLQ
AESYISNATEIQIQNMTTRLPFWKQVTVLTRRNFKLNFSDYVTLISTFAEPLIIGTVC
GWIYYKPDKSSIGGLRTTTACLYASTILQCYLYLLFDTYRLCEQDIALYDRERAEGSV
TPLAFIVARKISLFLSDDFAMTMIFVSITYFMFGLEADARKFFYQFAVVFLCQLSCSG
LSMLSVAVSRDFSKASLVGNMTFTVLSMGCGFFVNAKVMPVYVRWIKYIAFTWYSFGT
LMSSTFTNSYCTTDNLDECLGNQILEVYGFPRNWITVPAVVLLCWSVGYFVVGAIILY
LHKIDITLQNEVKSKQKKIKKKSPTGMKPEIQLLDDVYHQKDLEAEKGKNIHITIKLE
DIDLRVIFSAPFSNWKEGNFHHETKEILQSVNAIFKPGMINAIMGPSGSGKSSLLNLI
SGRLKSSVFAKFDTSGSIMFNDIQVSELMFKNVCSYVSQDDDHLLAALTVKETLKYAA
ALRLHHLTEAERMERTDNLIRSLGLKHCENNIIGNEFVKGISGGEKRRVTMGVQLLND
PPILLLDEPTSGLDSFTSATILEILEKLCREQGKTIIITIHQPRSELFKRFGNVLLLA
KSGRTAFNGSPDEMIAYFTELGYNCPSFTNVADFFLDLISVNTQNEQNEISSRARVEK
ILSAWKANMDNESLSPTPISEKQQYSQESFFTEYSEFVRKPANLVLAYIVNVKRQFTT
TRRSFDSLMARIAQIPGLGVIFALFFAPVKHNYTSISNRLGLAQESTALYFVGMLGNL
ACYPTERDYFYEEYNDNVYGIAPFFLAYMTLELPLSALASVLYAVFTVLACGLPRTAG
NFFATVYCSFIVTCCGEALGIMTNTFFERPGFVVNCISIILSIGTQMSGLMSLGMSRV
LKGFNYLNPVGYTSMIIINFAFPGNLKLTCEDGGKNSDGTCEFANGHDVLVSYGLVRN
TQKYLGIIVCVAIIYRLIAFFILKAKLEWIKW"
gene complement(<193832..>194800)
/gene="DSC2"
/locus_tag="YOL073C"
/db_xref="GeneID:854081"
mRNA complement(<193832..>194800)
/gene="DSC2"
/locus_tag="YOL073C"
/product="Dsc2p"
/transcript_id="NM_001183328.1"
/db_xref="GeneID:854081"
CDS complement(193832..194800)
/gene="DSC2"
/locus_tag="YOL073C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0044695 Dsc E3
ubiquitin ligase complex [PMID:22681890]"
/experiment="EXISTENCE:genetic interaction:GO:0140624 EGAD
pathway [PMID:31368600]"
/experiment="EXISTENCE:mutant phenotype:GO:0140624 EGAD
pathway [PMID:31368600]"
/note="Multi-transmembrane subunit of the DSC ubiquitin
ligase complex; involved in the endosome and
Golgi-associated degradation pathway (EGAD), contributing
to proteostasis and lipid homeostasis; similar in sequence
to rhomboid pseudoproteases Der1p and UBAC2 that function
in ERAD; ortholog of fission yeast dsc2"
/codon_start=1
/product="Dsc2p"
/protein_id="NP_014568.1"
/db_xref="GeneID:854081"
/db_xref="SGD:S000005434"
/translation="MSMEPPVGLTAMPVTKLAMITTLVVPLVASIASYKHIFLLQYDP
FLQTYHQYYRLLIFQFCAINESDTVILALIWYLFRHLERLLGSHKYLTLIVLSWAYTT
LGIWGLNLIWNAFIGQYKWLQWNNFSTGSLPIVLSLVHFYKEYTPQIYEWNIRLLGPR
GGASSHNDNKREDKSAVEWKINDQFLLNGLILLLILNQGFAGILCGFISWMCGIFIDK
GLLPGLDHWRIPFVSYFISQGPPTRANVAIAANAATNTAAARATVEAATAATGNGNTG
NSGPTSLPLRGSSTTPTNTSSAGDDEPGADEPARPLGVQFLDTFRR"
gene <194970..>196337
/gene="THP1"
/locus_tag="YOL072W"
/gene_synonym="BUD29"
/db_xref="GeneID:854082"
mRNA <194970..>196337
/gene="THP1"
/locus_tag="YOL072W"
/gene_synonym="BUD29"
/product="Thp1p"
/transcript_id="NM_001183327.1"
/db_xref="GeneID:854082"
CDS 194970..196337
/gene="THP1"
/locus_tag="YOL072W"
/gene_synonym="BUD29"
/experiment="EXISTENCE:direct assay:GO:0003690
double-stranded DNA binding [PMID:12702719]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:12702719]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11139493]"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:12206772]"
/experiment="EXISTENCE:direct assay:GO:0070390
transcription export complex 2 [PMID:15311284]"
/experiment="EXISTENCE:mutant phenotype:GO:0000282
cellular bud site selection [PMID:11452010]"
/experiment="EXISTENCE:mutant phenotype:GO:0000973
post-transcriptional tethering of RNA polymerase II gene
DNA at nuclear periphery [PMID:18003937]"
/experiment="EXISTENCE:mutant phenotype:GO:0006283
transcription-coupled nucleotide-excision repair
[PMID:17537816]"
/experiment="EXISTENCE:mutant phenotype:GO:0006368
transcription elongation by RNA polymerase II
[PMID:11139493]"
/experiment="EXISTENCE:mutant phenotype:GO:0006406 mRNA
export from nucleus [PMID:12411502]"
/experiment="EXISTENCE:mutant phenotype:GO:0031124 mRNA
3'-end processing [PMID:18614048]"
/experiment="EXISTENCE:mutant phenotype:GO:0071028 nuclear
mRNA surveillance [PMID:18003937]"
/note="Nuclear pore-associated protein; component of
TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in
transcription elongation and mRNA export from the nucleus;
involved in post-transcriptional tethering of active genes
to the nuclear periphery and to non-nascent mRNP; contains
a PAM domain implicated in protein-protein binding"
/codon_start=1
/product="Thp1p"
/protein_id="NP_014569.1"
/db_xref="GeneID:854082"
/db_xref="SGD:S000005433"
/translation="MDMANQLLDELAHGNFSHLTLNLSQNGREIAILQKQLTGFDDKQ
LETFVEQHPAMPNDTRFKIMCTSFLNYARDVDPWSAWSSSDLIFEFYQCLINCLINDN
APHIEMLIPVATRETEFIINLAGKLDSFHLQLHTRSHQFLSHISSILSRLFNSIKPPR
GNASSTNIPGKQRILLYLVNKLNNIYFRIESPQLCSNIFKNFQPKSMLAHFNEYQLDQ
QIEYRYLLGRYYLLNSQVHNAFVQFNEAFQSLLNLPLTNQAITRNGTRILNYMIPTGL
ILGKMVKWGPLRPFLSQETIDNWSVLYKHVRYGNIQGVSLWLRQNERHLCARQLLIVL
LEKLPMVTYRNLIKTVIKSWTTEWGQNKLPYSLIERVLQLSIGPTFEDPGAQEITIYN
GIHSPKNVENVLVTLINLGLLRANCFPQLQLCVVKKTTMIQEIVPPVNERITKMFPAH
SHVLW"
gene <196507..>196995
/gene="SDH5"
/locus_tag="YOL071W"
/gene_synonym="EMI5"
/db_xref="GeneID:854083"
mRNA <196507..>196995
/gene="SDH5"
/locus_tag="YOL071W"
/gene_synonym="EMI5"
/product="succinate dehydrogenase assembly factor SDH5"
/transcript_id="NM_001183326.1"
/db_xref="GeneID:854083"
CDS 196507..196995
/gene="SDH5"
/locus_tag="YOL071W"
/gene_synonym="EMI5"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:14562095|PMID:24769239|PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:19628817]"
/experiment="EXISTENCE:direct assay:GO:0017013 protein
flavinylation [PMID:19628817]"
/experiment="EXISTENCE:mutant phenotype:GO:0030437
ascospore formation [PMID:12586695]"
/experiment="EXISTENCE:mutant phenotype:GO:0034553
mitochondrial respiratory chain complex II assembly
[PMID:23043141]"
/experiment="EXISTENCE:physical interaction:GO:0006099
tricarboxylic acid cycle [PMID:19628817]"
/note="Protein required for flavinylation of Sdh1p; binds
to Sdh1p and promotes FAD cofactor attachment, which is
necessary for succinate dehydrogenase (SDH) complex
assembly and activity; mutations in human ortholog PGL2
are associated with neuroendocrine tumors (paraganglioma)"
/codon_start=1
/product="succinate dehydrogenase assembly factor SDH5"
/protein_id="NP_014570.1"
/db_xref="GeneID:854083"
/db_xref="SGD:S000005432"
/translation="MHNMFPALTKTLSLQGYKIINSQTGSAAWSCGRRWFSSDKDDHD
DVVTRIKIAPIKRTNEPLDKKRARLIYQSRKRGILETDLLLSGFAAKYLKKMNEEELE
EYDSLLNELDWDIYYWATKNFKTSPLPDKWANSKLLKQLQEFSENKEKEILSMPDLSK
YQ"
gene complement(<197220..>198725)
/gene="NBA1"
/locus_tag="YOL070C"
/db_xref="GeneID:854084"
mRNA complement(<197220..>198725)
/gene="NBA1"
/locus_tag="YOL070C"
/product="Nba1p"
/transcript_id="NM_001183325.1"
/db_xref="GeneID:854084"
CDS complement(197220..198725)
/gene="NBA1"
/locus_tag="YOL070C"
/experiment="EXISTENCE:direct assay:GO:0005621 cellular
bud scar [PMID:25416945|PMID:30341817]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:18086883|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:18086883|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0032153 cell
division site [PMID:25416945]"
/experiment="EXISTENCE:direct assay:GO:0032174 cellular
bud neck septin collar [PMID:30341817]"
/experiment="EXISTENCE:direct assay:GO:0032177 cellular
bud neck split septin rings [PMID:30341817]"
/experiment="EXISTENCE:mutant phenotype:GO:0005621
cellular bud scar [PMID:29074565]"
/experiment="EXISTENCE:mutant phenotype:GO:0007120 axial
cellular bud site selection [PMID:29074565]"
/experiment="EXISTENCE:mutant phenotype:GO:0045184
establishment of protein localization [PMID:29074565]"
/experiment="EXISTENCE:mutant phenotype:GO:1901900
regulation of protein localization to cell division site
[PMID:28148650]"
/note="Protein involved in axial bud site selection;
prevents repolarization of cells at previous division
sites; recruited by Aim44p and Nap1p to the cell cortex
and to cytokinesis remnants (bud scars) where it prevents
Rsr1p-mediated activation of Cdc24p, the Cdc42p GEF;
localizes to the cytoplasm, and the cell division site
prior to being stably recruited to cytokinesis remnants;
establishes the transient localization of Rga1p, a Cdc42p
GAP, at bud scars along with Nis1p; potential Cdc28p
substrate"
/codon_start=1
/product="Nba1p"
/protein_id="NP_014571.1"
/db_xref="GeneID:854084"
/db_xref="SGD:S000005431"
/translation="MSEEREENGISRATLNTQRLSAMIDSLNNEKDDRLFPSPTTTRT
MITEEKADQSDVFKPPSRLLRSPAGDVSLPPGDNRSSMISNYSGIIQEGVEVSYVVKN
RQQTQERRTSKDSNSLYSLKEPVSKNELPSLPMLPSEATLTKHLSDNQSTKSNTNADE
IVIKPVTNAKPVGRFNSNTSKKVEGRGSLKLLSSPLRQEKVMRSSIGSGNLASESGSS
TYNTKFHQSIQEQLEEEEEGNVSDKLSIVSSVIPELYTTTNEAPKAINPIRSETNDYN
PTIPPRSKDRPRSRLFIEEGDGEGDLLTEEILPTPVQPGGHYKNSSQISTVSEQKSES
YYSAATSMPPEEETYLTRPLPSTPNEDSRVTSNLKRDDTLKAIHDRANHTSTSTNKQD
DDMYEDIIEETPKKTKLKKDTKKKLNKKKSVKELRSFDIDTLNQLLSVTKGTLIGSEF
AQLGMKIEEKRALERLVDSLSRLTADMVLDPDRYEEGLKRLDKATKALEGF"
gene <198942..>200297
/gene="NUF2"
/locus_tag="YOL069W"
/db_xref="GeneID:854085"
mRNA <198942..>200297
/gene="NUF2"
/locus_tag="YOL069W"
/product="kinetochore-associated Ndc80 complex subunit
NUF2"
/transcript_id="NM_001183324.1"
/db_xref="GeneID:854085"
CDS 198942..200297
/gene="NUF2"
/locus_tag="YOL069W"
/experiment="EXISTENCE:direct assay:GO:0000776 kinetochore
[PMID:11179222|PMID:11511347]"
/experiment="EXISTENCE:direct assay:GO:0000779 condensed
chromosome, centromeric region [PMID:11266451]"
/experiment="EXISTENCE:direct assay:GO:0005816 spindle
pole body [PMID:8188751]"
/experiment="EXISTENCE:direct assay:GO:0005876 spindle
microtubule [PMID:11266451]"
/experiment="EXISTENCE:direct assay:GO:0008017 microtubule
binding [PMID:17195848]"
/experiment="EXISTENCE:direct assay:GO:0031262 Ndc80
complex [PMID:11266451]"
/experiment="EXISTENCE:genetic interaction:GO:0007059
chromosome segregation [PMID:11266451]"
/experiment="EXISTENCE:mutant phenotype:GO:0000776
kinetochore [PMID:11179222]"
/experiment="EXISTENCE:physical interaction:GO:0031262
Ndc80 complex [PMID:11179222]"
/note="Component of the kinetochore-associated Ndc80
complex; involved in chromosome segregation, spindle
checkpoint activity, and kinetochore clustering;
evolutionarily conserved; other members include Ndc80p,
Nuf2p, Spc24p, and Spc25p"
/codon_start=1
/product="kinetochore-associated Ndc80 complex subunit
NUF2"
/protein_id="NP_014572.1"
/db_xref="GeneID:854085"
/db_xref="SGD:S000005430"
/translation="MSRNQDVFPILDLQELVICLQSCDFALATQENISRPTSDYMVTL
YKQIIENFMGISVESLLNSSNQETGDGHLQEENENIYLDTLNVLVLNKICFKFFENIG
VQDFNMTDLYKPEAQRTQRLLSAVVNYARFREERMFDCNSFILQMESLLGQLRSKFDD
YNLIQQQLKQYEDVDGDNIPDEQELQKLEEQNKELEIQLKKLTKIQETLSIDYNDYKI
SKQSIFKDLEALSFQIVELESNRDKLIKISNTDMEELSEGIKELNDLLIQRKKTLDDL
TAQQKNLQDTVTTFETIISELYDVLRIISSEVQESNRTETELVGLKQNLINNKLKLMN
VLETGIMYKLEILQEQLDLQLKNLEKLSQDTKEESRLNDTKLMDLQIKYENEIKPKID
KTDIFIQEELISGKINKLNDEIKQLQKDFEVEVKEIEIEYSLLSGHINKYMNEMLEYM
Q"
gene complement(<200368..>201879)
/gene="HST1"
/locus_tag="YOL068C"
/db_xref="GeneID:854086"
mRNA complement(<200368..>201879)
/gene="HST1"
/locus_tag="YOL068C"
/product="histone deacetylase HST1"
/transcript_id="NM_001183323.1"
/db_xref="GeneID:854086"
CDS complement(200368..201879)
/gene="HST1"
/locus_tag="YOL068C"
/EC_number="2.3.1.286"
/experiment="EXISTENCE:direct assay:GO:0017136 histone
deacetylase activity, NAD-dependent
[PMID:11313477|PMID:11711434]"
/experiment="EXISTENCE:direct assay:GO:0034967 Set3
complex [PMID:11711434]"
/experiment="EXISTENCE:genetic interaction:GO:0030466
silent mating-type cassette heterochromatin formation
[PMID:27185881|PMID:11313477]"
/experiment="EXISTENCE:mutant phenotype:GO:0030466 silent
mating-type cassette heterochromatin formation
[PMID:11313477]"
/experiment="EXISTENCE:mutant phenotype:GO:0045950
negative regulation of mitotic recombination
[PMID:16051752]"
/note="NAD(+)-dependent histone deacetylase; essential
subunit of the Sum1p/Rfm1p/Hst1p complex required for
ORC-dependent silencing and meiotic repression;
non-essential subunit of the Set3C deacetylase complex;
involved in telomere maintenance; HST1 has a paralog,
SIR2, that arose from the whole genome duplication"
/codon_start=1
/product="histone deacetylase HST1"
/protein_id="NP_014573.1"
/db_xref="GeneID:854086"
/db_xref="SGD:S000005429"
/translation="MNILLMQRIVSFILVVSQGRYFHVGELTMTMLKRPQEEESDNNA
TKKLKTRLTYPCILGKDKVTGKFIFPAITKDDVMNARLFLKDNDLKTFLEYFLPVEVN
SIYIYFMIKLLGFDVKDKELFMALNSNITSNKERSSAELSSIHAKAEDEDELTDPLEK
KHAVKLIKDLQKAINKVLSTRLRLPNFNTIDHFTATLRNAKKILVLTGAGVSTSLGIP
DFRSSEGFYSKIRHLGLEDPQDVFNLDIFLQDPSVFYNIAHMVLPPENMYSPLHSFIK
MLQDKGKLLRNYTQNIDNLESYAGIDPDKLVQCHGSFATASCVTCHWQIPGEKIFENI
RNLELPLCPYCYQKRKQYFPMSNGNNTVQTNINFNSPILKSYGVLKPDMTFFGEALPS
RFHKTIRKDILECDLLICIGTSLKVAPVSEIVNMVPSHVPQILINRDMVTHAEFDLNL
LGFCDDVASLVAKKCHWDIPHKKWQDLKKIDYNCTEIDKGTYKIKKQPRKKQQ"
gene complement(<201985..>202518)
/gene="RTG1"
/locus_tag="YOL067C"
/db_xref="GeneID:854087"
mRNA complement(<201985..>202518)
/gene="RTG1"
/locus_tag="YOL067C"
/product="Rtg1p"
/transcript_id="NM_001183322.1"
/db_xref="GeneID:854087"
CDS complement(201985..202518)
/gene="RTG1"
/locus_tag="YOL067C"
/experiment="EXISTENCE:direct assay:GO:0000981 DNA-binding
transcription factor activity, RNA polymerase II-specific
[PMID:7493987|PMID:8422683]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10848632]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:10848632]"
/experiment="EXISTENCE:direct assay:GO:0031930
mitochondria-nucleus signaling pathway [PMID:7493987]"
/experiment="EXISTENCE:direct assay:GO:0045944 positive
regulation of transcription by RNA polymerase II
[PMID:7493987]"
/experiment="EXISTENCE:mutant phenotype:GO:0000981
DNA-binding transcription factor activity, RNA polymerase
II-specific [PMID:7493987|PMID:8422683]"
/experiment="EXISTENCE:mutant phenotype:GO:0031930
mitochondria-nucleus signaling pathway [PMID:8422683]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:8422683|PMID:12393187]"
/experiment="EXISTENCE:mutant phenotype:GO:0071400
cellular response to oleic acid [PMID:12393187]"
/note="Transcription factor (bHLH) involved in
interorganelle communication; contributes to communication
between mitochondria, peroxisomes, and nucleus; target of
Hog1p; activated in stochastic pulses of nuclear
localization"
/codon_start=1
/product="Rtg1p"
/protein_id="NP_014574.1"
/db_xref="GeneID:854087"
/db_xref="SGD:S000005428"
/translation="MSSIPAGTDPGSCGANFKNDRKRRDKINDRIQELLSIIPKDFFR
DYYGNSGSNDTLSESTPGALGLSSKAKGTGTKDGKPNKGQILTQAVEYISHLQNQVDT
QNREEVELMVKATQLAKQTGTIVNDINLENTSAEVALSRIGVGPLAATNDDSVRPPAK
RLSSFEYGGYGEYGNGS"
gene complement(<202696..>204471)
/gene="RIB2"
/locus_tag="YOL066C"
/gene_synonym="PUS8"
/db_xref="GeneID:854088"
mRNA complement(<202696..>204471)
/gene="RIB2"
/locus_tag="YOL066C"
/gene_synonym="PUS8"
/product="bifunctional DRAP deaminase/tRNA pseudouridine
synthase RIB2"
/transcript_id="NM_001183321.1"
/db_xref="GeneID:854088"
CDS complement(202696..204471)
/gene="RIB2"
/locus_tag="YOL066C"
/gene_synonym="PUS8"
/EC_number="3.5.4.26"
/EC_number="5.4.99.28"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0009982
pseudouridine synthase activity [PMID:15466869]"
/experiment="EXISTENCE:direct assay:GO:0031119 tRNA
pseudouridine synthesis [PMID:15466869]"
/experiment="EXISTENCE:mutant phenotype:GO:0008835
diaminohydroxyphosphoribosylaminopyrimidine deaminase
activity [PMID:4555411]"
/experiment="EXISTENCE:mutant phenotype:GO:0009231
riboflavin biosynthetic process
[PMID:5366000|PMID:4555411]"
/experiment="EXISTENCE:mutant phenotype:GO:0009982
pseudouridine synthase activity [PMID:15466869]"
/experiment="EXISTENCE:mutant phenotype:GO:0031119 tRNA
pseudouridine synthesis [PMID:15466869]"
/note="Bifunctional DRAP deaminase tRNA:pseudouridine
synthase; the deaminase catalyzes the third step in
riboflavin biosynthesis and the synthase catalyzes
formation of pseudouridine at position 32 in cytoplasmic
tRNAs; RIB2 has a paralog, PUS9, that arose from the whole
genome duplication"
/codon_start=1
/product="bifunctional DRAP deaminase/tRNA pseudouridine
synthase RIB2"
/protein_id="NP_014575.1"
/db_xref="GeneID:854088"
/db_xref="SGD:S000005427"
/translation="MEDSNNEASDDFNNLLNKEIESAKEVKLRKFANRNNNRNENSSK
VKDASGFRLRVIQTDGHKTKKTDPDYEVTIDGPLRKIEPYFFTYKTFCKERWRDRKLV
DVFVSEFRDREPSYYSKTIAEGKVYLNDEPANLDTIIRDGDLITHKVHRHEPPVTSKP
IDIVFEDEDILVIDKPSSIPVHPTGRYRFNTITKMLERQLGYSVHPCNRLDKPTSGLM
FLAKTPLGADRMGDQMKAREVTKEYVARVKGEFPIGIVEVDKPVRSVNPKVALNAVCE
MSDENAKHAKTVFQRVSYDGQTSIVKCKPLTGRTHQIRVHLQYLGFPIANDPIYSNPD
IWGPDLGRGGLQNYDDIVLKLDAIGKTNPAESWIHPHSEGEYLLGRQCEECEAEMYTD
PGTNDLDLWLHAFRYESLERNSDTQKPLWSYRTKYPEWALEPHRRYMEMAVKEAGKCG
PTKTAFSVGAVLVHGTQVLATGYSRELPGNTHAEQCALIKYSQLHPNCPTIVPMGTVL
YTTMEPCSFRLSGNEPCCDRILATQGAIGTVFVGVMEPDTFVKNNTSLNKLESHGVNY
IQIPGYEEECTIIAFKGHDNSDDKA"
gene complement(<204731..>205885)
/gene="INP54"
/locus_tag="YOL065C"
/db_xref="GeneID:854089"
mRNA complement(<204731..>205885)
/gene="INP54"
/locus_tag="YOL065C"
/product="phosphoinositide 5-phosphatase INP54"
/transcript_id="NM_001183320.1"
/db_xref="GeneID:854089"
CDS complement(204731..205885)
/gene="INP54"
/locus_tag="YOL065C"
/EC_number="3.1.3.36"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0004439
phosphatidylinositol-4,5-bisphosphate 5-phosphatase
activity [PMID:10660045]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:11116155]"
/experiment="EXISTENCE:direct assay:GO:0046856
phosphatidylinositol dephosphorylation [PMID:10660045]"
/note="Phosphatidylinositol 4,5-bisphosphate
5-phosphatase; role in secretion; localizes to the
endoplasmic reticulum via the C-terminal tail; lacks the
Sac1 domain and proline-rich region found in the other 3
INP proteins"
/codon_start=1
/product="phosphoinositide 5-phosphatase INP54"
/protein_id="NP_014576.1"
/db_xref="GeneID:854089"
/db_xref="SGD:S000005426"
/translation="MNKTNWKVSVTTFNCGKEFPVENSKAIVKQLLFPYDDGISQLEL
QDLYVLGFQEVVPIWQGSFPAVNRDLIDRITTTAVNCLNEKVSATQGDEQYSCLGVNS
LGAITIIVLYNNNALKVKDDILKRNGKCGWFGTHLKGGTLISFQMTRNGEENWERFSY
ICAHLNANEGVNNRNQRIDDYKRIMSEVCDSEVAKSDHFFFLGDLNFRVTSTYDPTTN
YSSTTTLRRLLENHEELNLLRKGEDEPLCKGFQELKITFPPTYKFKLFEKETYNTKRI
PSWCDRILYKSYAVPTFAQEGTYHSVPRSNALLFSDHQPVNLTVRLPRSTGTPVPLSL
HIEKYPLSWSSGLIGQIGDAVIGYCGWLVTKNVHYWILGSLLLYLLLKIL"
gene complement(<206103..>207176)
/gene="MET22"
/locus_tag="YOL064C"
/gene_synonym="HAL2"
/db_xref="GeneID:854090"
mRNA complement(<206103..>207176)
/gene="MET22"
/locus_tag="YOL064C"
/gene_synonym="HAL2"
/product="3'(2'),5'-bisphosphate nucleotidase"
/transcript_id="NM_001183319.1"
/db_xref="GeneID:854090"
CDS complement(206103..207176)
/gene="MET22"
/locus_tag="YOL064C"
/gene_synonym="HAL2"
/EC_number="3.1.3.7"
/experiment="EXISTENCE:direct assay:GO:0008441
3'(2'),5'-bisphosphate nucleotidase activity
[PMID:7809627]"
/experiment="EXISTENCE:direct assay:GO:0016078 tRNA decay
[PMID:31619505]"
/experiment="EXISTENCE:mutant phenotype:GO:0000103 sulfate
assimilation [PMID:8910555]"
/experiment="EXISTENCE:mutant phenotype:GO:0009086
methionine biosynthetic process [PMID:8393782]"
/experiment="EXISTENCE:mutant phenotype:GO:0042538
hyperosmotic salinity response [PMID:8393782]"
/note="Bisphosphate-3'-nucleotidase; involved in salt
tolerance and methionine biogenesis; dephosphorylates
3'-phosphoadenosine-5'-phosphate and
3'-phosphoadenosine-5'-phosphosulfate, intermediates of
the sulfate assimilation pathway; human homolog BPNT1
complements yeast null mutant"
/codon_start=1
/product="3'(2'),5'-bisphosphate nucleotidase"
/protein_id="NP_014577.1"
/db_xref="GeneID:854090"
/db_xref="SGD:S000005425"
/translation="MALERELLVATQAVRKASLLTKRIQSEVISHKDSTTITKNDNSP
VTTGDYAAQTIIINAIKSNFPDDKVVGEESSSGLSDAFVSGILNEIKANDEVYNKNYK
KDDFLFTNDQFPLKSLEDVRQIIDFGNYEGGRKGRFWCLDPIDGTKGFLRGEQFAVCL
ALIVDGVVQLGCIGCPNLVLSSYGAQDLKGHESFGYIFRAVRGLGAFYSPSSDAESWT
KIHVRHLKDTKDMITLEGVEKGHSSHDEQTAIKNKLNISKSLHLDSQAKYCLLALGLA
DVYLRLPIKLSYQEKIWDHAAGNVIVHEAGGIHTDAMEDVPLDFGNGRTLATKGVIAS
SGPRELHDLVVSTSCDVIQSRNA"
gene complement(<207392..>210265)
/gene="CRT10"
/locus_tag="YOL063C"
/db_xref="GeneID:854091"
mRNA complement(<207392..>210265)
/gene="CRT10"
/locus_tag="YOL063C"
/product="Crt10p"
/transcript_id="NM_001183318.1"
/db_xref="GeneID:854091"
CDS complement(207392..210265)
/gene="CRT10"
/locus_tag="YOL063C"
/experiment="EXISTENCE:genetic interaction:GO:0070651
nonfunctional rRNA decay [PMID:25534857]"
/experiment="EXISTENCE:mutant phenotype:GO:0070651
nonfunctional rRNA decay [PMID:25534857]"
/note="Protein required for the degradation of mutant 25S
rRNAs; functions in Mms1 pathway of nonfunctional 25S rRNA
decay; expression induced by DNA damage and null mutations
confer increased resistance to hydroxyurea; N-terminal
region has a leucine repeat and a WD40 repeat"
/codon_start=1
/product="Crt10p"
/protein_id="NP_014578.1"
/db_xref="GeneID:854091"
/db_xref="SGD:S000005424"
/translation="MPPQIPNENDDLFTRWLKSRAIIQRAVSTRECFDSEVFLASGGW
NITNEIITLKKYYQLKWPNSSCNSFHPKTVEFIKERLHNLEEHDSSWKIPNPAYSFKK
AFLEDTKSAFSNLEPVWGPSRLLNPAELLLPQDEKLLVQEIPLEFAPFQYTNRFAYGG
LQFKNNLFVTYGSYSFLAAGQCVEVHNFDILLNVSSLEICHALLPVIIPDDGDVRNFR
NSSYVKFKDTQFNSIPELCSINFMKICNFMHQDFLLACGDNGIVYIWEINKVIKIFNK
FTSDILGGKDNSRERYINVDPYMVLRVEESCWSVDVIDINGIIYIAVGHNKPGVTVFA
FDKDVKKERRYIRPLDLPSSHNVPCVNFVPNSKDSVGYITLSYCSIFGNVVTVKLKEH
DCTILTSFLDTQFFGDDLWTITPLTKKDFAKVDNFELLNLNYQDGFKESMLYSICRDD
FLLGYYCDNAYLSGNFGIGTLLNQFQVPVTDLRLTSSAGIPDEVIPLRFTSFDRNYTT
TGSIKYEYSREDFALILHAGDLDDMNDAVTKNTSCEQHLHQWTFWEDSGYKHYRATER
GFSKYKDIINTFPQLITPSGRNKTSQYQNTSGRKICEPSTYKLTDLENDIEDISREFN
RSIRNLKMDKQRQLRTSKEFKSLSSVNHIPNIESGNFLWYNTDAAADWRTLFGKDLNT
VLKDPEICSLQLNSTEEDDVNSDPENEESGSSLTSFQRRYRDTEQRAHLKSESQKSWG
FHNYVRNVKRLLESAVPGSEDSPLGYQLSEMHDEFFFLTTAHRLVLMKANPLIIISAT
HHEIFPLDGVVTCASKSLLQALNRINFVCHIKELNCIAVASQLGLISLLRLTEYRGIY
SFRQEYILGWEVQDPVNPSPECRCNRNLFDAPMYGADGESSDTYCGVCDVYFPMGDIC
GLDYTYASDSEELKRKGYATLYVASRGSLRAFKITTEHGTTQQ"
gene complement(<210520..>211995)
/gene="APM4"
/locus_tag="YOL062C"
/gene_synonym="AMP1"
/db_xref="GeneID:854092"
mRNA complement(<210520..>211995)
/gene="APM4"
/locus_tag="YOL062C"
/gene_synonym="AMP1"
/product="Apm4p"
/transcript_id="NM_001183317.1"
/db_xref="GeneID:854092"
CDS complement(210520..211995)
/gene="APM4"
/locus_tag="YOL062C"
/gene_synonym="AMP1"
/experiment="EXISTENCE:curator inference:GO:0006886
intracellular protein transport [PMID:10564262]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:24460703]"
/experiment="EXISTENCE:physical interaction:GO:0030122
AP-2 adaptor complex [PMID:10564262]"
/note="Cargo-binding mu subunit of AP-2; AP-2 is a
heterotetrameric endocytic cargo-binding adaptor that
facilitates uptake of membrane proteins during
clathrin-mediated endocytosis; Apm4p is required for AP-2
function and localization, and binds cell wall stress
receptor Mid2p; AP-2 is required for cell polarity
responses to pheromone, nutritional status and cell wall
damage in S. cerevisiae, and for hyphal growth in C.
albicans; AP-2 complex is conserved in mammals"
/codon_start=1
/product="Apm4p"
/protein_id="NP_014579.1"
/db_xref="GeneID:854092"
/db_xref="SGD:S000005423"
/translation="MISGVLVYSSRGELVLNKFFKNSLKRSISDIFRVQVINNLDVRS
PVLTLGSTTFHHIRSRHGDNLWLVTITRSNANSAAIWEFLYKLDAVMNAYRLDREEAL
KEEFMIVHEMLDIMLGGNGIPIDTELNSVIAQMSVKPVRNMGGLLDSPDGNDVLSSSS
SPTSSAGELHFPKFLTKRSSSFLGQGDSTSDFYDNNKITWRPKGIIHKKDEVFLYVNE
RINILVSRDGSILKSYVDGTIDITTHLSGTPICRFGLNDSLGMQSEDEKKWLAQQQRH
SGSDFGNKNFIPKAAAGSVLLEDCKFHECVSLDKFNRNHIIEFVPPDGSMELMKYHVR
DNINLPFKVTPIVTHSTRDNEIDYRITLKSLFPGKLSAKDVVLHIPVPPSTVDCKISV
SNGHCKFVPEENAMIWRFNKYNGLTENTLSAVTVSTSDTTQLNLQQWTRPPISLEFEV
MMFSNSGLVVRYFTISGKDSKHRAVKWIKYISKAGSYEVRY"
gene <212244..>213734
/gene="PRS5"
/locus_tag="YOL061W"
/db_xref="GeneID:854093"
mRNA <212244..>213734
/gene="PRS5"
/locus_tag="YOL061W"
/product="ribose phosphate diphosphokinase subunit PRS5"
/transcript_id="NM_001183316.1"
/db_xref="GeneID:854093"
CDS 212244..213734
/gene="PRS5"
/locus_tag="YOL061W"
/EC_number="2.7.6.1"
/experiment="EXISTENCE:direct assay:GO:0002189 ribose
phosphate diphosphokinase complex [PMID:15280369]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0097268
cytoophidium [PMID:39836171]"
/experiment="EXISTENCE:genetic interaction:GO:0004749
ribose phosphate diphosphokinase activity [PMID:15280369]"
/experiment="EXISTENCE:genetic interaction:GO:0006015
5-phosphoribose 1-diphosphate biosynthetic process
[PMID:15280369]"
/experiment="EXISTENCE:mutant phenotype:GO:0006015
5-phosphoribose 1-diphosphate biosynthetic process
[PMID:10212224]"
/note="5-phospho-ribosyl-1(alpha)-pyrophosphate
synthetase; synthesizes PRPP, which is required for
nucleotide, histidine, and tryptophan biosynthesis; one of
five related enzymes, which are active as heteromultimeric
complexes; forms cytoplasmic foci upon DNA replication
stress"
/codon_start=1
/product="ribose phosphate diphosphokinase subunit PRS5"
/protein_id="NP_014580.1"
/db_xref="GeneID:854093"
/db_xref="SGD:S000005422"
/translation="MSMSNIVVFGGDSHPELVTKICENLDIHPSKVELGKFSNGETNI
ALRESVREKDVYIIQSGCGQVNDTFMQLLILISACKSASASRVTAVMPYLCYSRQPDI
PYTAKGAPIISKPKENYTFESHPGTPVSSSLMTQRPGAESSLKSLDSAIRSTINLENP
QPIRTPNSSATANNNFDIKKTLSFSRIPMIPGGKLQNTSNSTDAGELFNAQNAGYKLW
VVQAGTLIAHLLSAAGADHVITMDLHDPQFPGFFDIPVDNLYCKPIAQNYIQHRIPDY
QDAVIVSPDAGGAKRATAIADALELSFALIHKERRSQLLKGPPDATLTSGGSLPVSPR
PLVTTLVSSQNTTSSGATGVAALEMKKTTSTSSTSSQSSNSSKFVQTTMLVGDVRNKV
CIIVDDLVDTSYTITRAAKLLKDQGSTKVYALITHGVFSGDALERIGQSSIDKLIISN
TVPQDRTLQYLGKDRVDVIDVSCIIGEAIRRIHNGESISMLFEHGW"
gene complement(<214017..>216137)
/gene="MAM3"
/locus_tag="YOL060C"
/db_xref="GeneID:854094"
mRNA complement(<214017..>216137)
/gene="MAM3"
/locus_tag="YOL060C"
/product="Mam3p"
/transcript_id="NM_001183315.1"
/db_xref="GeneID:854094"
CDS complement(214017..216137)
/gene="MAM3"
/locus_tag="YOL060C"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0007005
mitochondrion organization [PMID:10628851]"
/experiment="EXISTENCE:mutant phenotype:GO:0010961
intracellular magnesium ion homeostasis [PMID:35087208]"
/experiment="EXISTENCE:mutant phenotype:GO:0030026
intracellular manganese ion homeostasis [PMID:15498024]"
/note="Protein involved in magnesium homeostasis; required
for Mg2+ sequestration in vacuole; required for normal
mitochondrial morphology; has similarity to hemolysins"
/codon_start=1
/product="Mam3p"
/protein_id="NP_014581.1"
/db_xref="GeneID:854094"
/db_xref="SGD:S000005421"
/translation="MSFLPLRSRSRSGAPHWVYIILYHIFTIPKIYSLPLLSGSHVLN
SRDVADSGHSVGDEASVTTYYIISIILVLLGGVFAGLTLGLMGQDEVYLKVISTSGSN
SEKKLAKRVLDLISRGKHWVLVTLLLSNVITNETLPIVLDRCLGGGWQAVVSSTILIV
IFGEIIPQSVCVKYGLQVGAFFCPFVLVLMYLMYPVAYPIATLLDYMLGEDHGTMYKK
SGLKTLVTLHRTMGVERLTKDEVTIISAVLDLKAKRVEEIMTPIENVFTMSADTILDD
KTVEKIFNSGFSRIPIFLPNEPNNFIGMLLVRVLISYDPDDCLPISHFPLATLPETSP
NTSCLNILNYFQEGKAHMCVVSKEPGSSHGAIGVLTLEDVIEELIGEEIVDESDVFVD
MHQHIMRQQPGPLSKRHITSYLHHLYTSSHKEHKAADQADESSPLLSPSNSNHPSEHP
QQDLNNKSWKQKSNDGYDRSNAVLSPTPQVTEHGTIIPSNLASNPLNVNKSFVTIKKP
ANVPKIITTHTPHSSKEPSPAPHSNDKSLSAEEQQLLSDHAELSRQAVLHTQRSGQPT
QVTTSTKTTRNSPDSISIPNSGANHGNENQNVTISTSYQNTKNGIVESVITVKGVPKT
IIGPAKDWDESKSEYGNENINQENSNRSDDRESSSSNASLFSSIKNKFKNENANNNDR
SNFTDSLSRTSNYDANGSSSTIKR"
gene <217126..>218448
/gene="GPD2"
/locus_tag="YOL059W"
/gene_synonym="GPD3"
/db_xref="GeneID:854095"
mRNA <217126..>218448
/gene="GPD2"
/locus_tag="YOL059W"
/gene_synonym="GPD3"
/product="glycerol-3-phosphate dehydrogenase (NAD(+))
GPD2"
/transcript_id="NM_001183314.1"
/db_xref="GeneID:854095"
CDS 217126..218448
/gene="GPD2"
/locus_tag="YOL059W"
/gene_synonym="GPD3"
/EC_number="1.1.1.8"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:17054397|PMID:24769239|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:mutant phenotype:GO:0006071
glycerol metabolic process [PMID:9171333]"
/experiment="EXISTENCE:mutant phenotype:GO:0047952
glycerol-3-phosphate dehydrogenase [NAD(P)+] activity
[PMID:9171333]"
/note="NAD-dependent glycerol 3-phosphate dehydrogenase;
expression is controlled by an oxygen-independent
signaling pathway required to regulate metabolism under
anoxic conditions; located in cytosol and mitochondria;
constitutively active but is inactivated via
phosphorylation by energy-stress responsive kinase SNF1;
GPD2 has a paralog, GPD1, that arose from the whole genome
duplication"
/codon_start=1
/product="glycerol-3-phosphate dehydrogenase (NAD(+))
GPD2"
/protein_id="NP_014582.1"
/db_xref="GeneID:854095"
/db_xref="SGD:S000005420"
/translation="MLAVRRLTRYTFLKRTHPVLYTRRAYKILPSRSTFLRRSLLQTQ
LHSKMTAHTNIKQHKHCHEDHPIRRSDSAVSIVHLKRAPFKVTVIGSGNWGTTIAKVI
AENTELHSHIFEPEVRMWVFDEKIGDENLTDIINTRHQNVKYLPNIDLPHNLVADPDL
LHSIKGADILVFNIPHQFLPNIVKQLQGHVAPHVRAISCLKGFELGSKGVQLLSSYVT
DELGIQCGALSGANLAPEVAKEHWSETTVAYQLPKDYQGDGKDVDHKILKLLFHRPYF
HVNVIDDVAGISIAGALKNVVALACGFVEGMGWGNNASAAIQRLGLGEIIKFGRMFFP
ESKVETYYQESAGVADLITTCSGGRNVKVATYMAKTGKSALEAEKELLNGQSAQGIIT
CREVHEWLQTCELTQEFPLFEAVYQIVYNNVRMEDLPEMIEELDIDDE"
gene <219211..>220473
/gene="ARG1"
/locus_tag="YOL058W"
/gene_synonym="ARG10"
/db_xref="GeneID:854096"
mRNA <219211..>220473
/gene="ARG1"
/locus_tag="YOL058W"
/gene_synonym="ARG10"
/product="argininosuccinate synthase"
/transcript_id="NM_001183313.2"
/db_xref="GeneID:854096"
CDS 219211..220473
/gene="ARG1"
/locus_tag="YOL058W"
/gene_synonym="ARG10"
/EC_number="6.3.4.5"
/experiment="EXISTENCE:direct assay:GO:0004055
argininosuccinate synthase activity [PMID:2897249]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:205532]"
/experiment="EXISTENCE:mutant phenotype:GO:0006526
L-arginine biosynthetic process [PMID:2897249]"
/note="Argininosuccinate synthetase; catalyzes the
formation of L-argininosuccinate from citrulline and
L-aspartate in the arginine biosynthesis pathway;
potential Cdc28p substrate"
/codon_start=1
/product="argininosuccinate synthase"
/protein_id="NP_014583.2"
/db_xref="GeneID:854096"
/db_xref="SGD:S000005419"
/translation="MSKGKVCLAYSGGLDTSVILAWLLDQGYEVVAFMANVGQEEDFD
AAKEKALKIGACKFVCVDCREDFVKDILFPAVQVNAVYEDVYLLGTSLARPVIAKAQI
DVAKQEGCFAVSHGCTGKGNDQIRFELSFYALKPDVKCITPWRMPEFFERFAGRKDLL
DYAAQKGIPVAQTKAKPWSTDENQAHISYEAGILEDPDTTPPKDMWKLIVDPMDAPDQ
PQDLTIDFERGLPVKLTYTDNKTSKEVSVTKPLDVFLAASNLARANGVGRIDIVEDRY
INLKSRGCYEQAPLTVLRKAHVDLEGLTLDKEVRQLRDSFVTPNYSRLIYNGSYFTPE
CEYIRSMIQPSQNSVNGTVRVRLYKGNVIILGRSTKTEKLYDPTESSMDELTGFLPTD
TTGFIAIQAIRIKKYGESKKTKGEELTL"
gene <220767..>222902
/locus_tag="YOL057W"
/db_xref="GeneID:854097"
mRNA <220767..>222902
/locus_tag="YOL057W"
/product="dipeptidyl-peptidase III"
/transcript_id="NM_001183312.1"
/db_xref="GeneID:854097"
CDS 220767..222902
/locus_tag="YOL057W"
/EC_number="3.4.14.4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008239
dipeptidyl-peptidase activity [PMID:19825391]"
/note="Dipeptidyl-peptidase III; cleaves dipeptides from
the amino terminus of target proteins; highly active on
synthetic substrate Arg-Arg-2-naphthylamide; mammalian
ortholog may be a biomarker for some cancers"
/codon_start=1
/product="dipeptidyl-peptidase III"
/protein_id="NP_014584.1"
/db_xref="GeneID:854097"
/db_xref="SGD:S000005418"
/translation="MSHFFADHDAPLSMLSVKTEYFPQLTDKEQKYAHFMSKASHAGS
RVVMRQVSHESEPIFDLILAIHSKLNGKYPEDDITQKQQTGLYLEYVSQFLSNLGNFK
SFGDTKFIPRCEVKFFKQLLELAKINPCSSPLTLSPVDVNHEFTSHHLFSTINELIDI
GIYHVEEKAALLGFPSQGYTSAYYLGLPVTPEDMALLKEQLFAELAILPENTRINKVG
ENSFQIWVASENVKNQITETYPSGQITLSNAVTKVEFIFGDHSREMRLVASYLKEAQK
FAANDTQKAMLQEYINHFVTGSSQAHKEAQKLWVKDISPVIETNIGFIETYREPSGII
GEFESLVAIQNKERTAKFSSLVNNAEEFISLLPWSKDYEKPIFNPPDFTSLEVLTFTG
SGIPAGINIPNYDDVRLKIGFKNVSLGNILSAAAKSSSKHPPSFISQEDRPIFEKYQS
DSFEVQVGIHELLGHGSGKLLTEFTDGFNFDKENPPLGLDGKPVSTYYKVGETWGSKF
GQLAGPFEECRAEVIAMFLLTNKKILDIFGFHDVESQDKVIYAGYLQMARAGLLALEY
WNPKTGKWGQPHMQARFSIMKTFMKHSTDKNFLKLEMNSTNDDFAIKLDKSLIKTAGH
ECVKDYLKHLHVYKCSGDVEQGSKYFIDRSTVTPDLASLRDIVLSKRLPRRQFIQSNS
YIDDNNKVTLKEYDETPQGMLQSFLDREL"
gene <223268..>224179
/gene="GPM3"
/locus_tag="YOL056W"
/db_xref="GeneID:854098"
mRNA <223268..>224179
/gene="GPM3"
/locus_tag="YOL056W"
/product="phosphoglycerate mutase family protein GPM3"
/transcript_id="NM_001183311.1"
/db_xref="GeneID:854098"
CDS 223268..224179
/gene="GPM3"
/locus_tag="YOL056W"
/EC_number="5.4.2.11"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="Nonfunctional homolog of Gpm1p phosphoglycerate
mutase; if functional, would convert 3-phosphoglycerate to
2-phosphoglycerate in glycolysis; GPM3 has a paralog,
GPM2, that arose from the whole genome duplication"
/codon_start=1
/product="phosphoglycerate mutase family protein GPM3"
/protein_id="NP_014585.1"
/db_xref="GeneID:854098"
/db_xref="SGD:S000005417"
/translation="MTVTDTFKLFILRHGQSELNSENIFCGWIDAQLTEKGKSQARHS
AKLIKQFCDSNNISLPQIGYTSRLIRTQQTMDVILEELGLKHTNYVITTNTNIKEELQ
DTRFEGSMPVLQTWRLNERHYGAWQGQRKPDILKEYGKEKYMYIRRDYNGKPPKVNLN
LEMVQEENDQGSSTGYDFKEPNRHLKYGPEEKANERLPESESLCEVVVRLKPFLNNVV
LSTANKISQESCVIVGHGSSVRSLLKVLEGISDEDIKDVDIPNGIPLVIELDRDNYSF
VRKFYLDPESAKVNAQMVRDEGFEKNP"
gene complement(<224420..>226075)
/gene="THI20"
/locus_tag="YOL055C"
/db_xref="GeneID:854099"
mRNA complement(<224420..>226075)
/gene="THI20"
/locus_tag="YOL055C"
/product="trifunctional hydroxymethylpyrimidine
kinase/phosphomethylpyrimidine kinase/thiaminase"
/transcript_id="NM_001183310.1"
/db_xref="GeneID:854099"
CDS complement(224420..226075)
/gene="THI20"
/locus_tag="YOL055C"
/EC_number="2.7.1.49"
/EC_number="2.7.4.7"
/EC_number="3.5.99.2"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:16850348]"
/experiment="EXISTENCE:direct assay:GO:0008902
hydroxymethylpyrimidine kinase activity
[PMID:15967475|PMID:15614489]"
/experiment="EXISTENCE:direct assay:GO:0008972
phosphomethylpyrimidine kinase activity [PMID:15614489]"
/experiment="EXISTENCE:direct assay:GO:0009230 thiamine
catabolic process [PMID:15967475]"
/experiment="EXISTENCE:direct assay:GO:0050334 thiaminase
activity [PMID:15967475]"
/experiment="EXISTENCE:genetic interaction:GO:0008902
hydroxymethylpyrimidine kinase activity [PMID:15614489]"
/experiment="EXISTENCE:genetic interaction:GO:0008972
phosphomethylpyrimidine kinase activity
[PMID:10383756|PMID:15614489]"
/experiment="EXISTENCE:mutant phenotype:GO:0009228
thiamine biosynthetic process [PMID:10383756]"
/note="Trifunctional enzyme of thiamine biosynthesis,
degradation and salvage; has hydroxymethylpyrimidine (HMP)
kinase, HMP-phosphate (HMP-P) kinase and thiaminase
activities; member of a gene family with THI21 and THI22;
HMP and HMP-P kinase activity redundant with Thi21p"
/codon_start=1
/product="trifunctional hydroxymethylpyrimidine
kinase/phosphomethylpyrimidine kinase/thiaminase"
/protein_id="NP_014586.1"
/db_xref="GeneID:854099"
/db_xref="SGD:S000005416"
/translation="MTYSTVSINTPPPYLTLACNEKLPTVLSIAGTDPSGGAGIEADV
KTITAHRCYAMTCITALNAQTPVKVYSINNTPKEVVFQTLESNLKDMKCNVIKTGMLT
AAAIEVLHEKLLQLGENRPKLVVDPVLVATSGSSLAGKDIVSLITEKVAPFADILTPN
IPECYKLLGEERKVNGLQDIFQIAKDLAKITKCSNILVKGGHIPWNDEKEKYITDVLF
LGAEQKFIIFKGNFVNTTHTHGTGCTLASAIASNLARGYSLPQSVYGGIEYVQNAVAI
GCDVTKETVKDNGPINHVYAVEIPLEKMLSDECFTASDVIPKKPLKSAADKIPGGNFY
EYLINHPKVKPHWDSYINHEFVKKVADGTLERKKFQFFIEQDYAYLVDYARVHCIAGS
KAPCLEDMEKELVIVGGVRTEMGQHEKRLKEVFGVKDPDYFQKIKRGPALRAYSRYFN
DVSRRGNWQELVASLTPCLMGYGEALTKMKGKVTAPEGSVYHEWCETYASSWYREAMD
EGEKLLNHILETYPPEQLDTLVTIYAEVCELETNFWTAALEYE"
gene complement(226611..226681)
/locus_tag="YNCO0004C"
/db_xref="GeneID:854100"
tRNA complement(226611..226681)
/locus_tag="YNCO0004C"
/product="tRNA-Gly"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0030371 translation
repressor activity [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043555 regulation
of translation in response to stress [PMID:27609601]"
/note="Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:854100"
/db_xref="SGD:S000006586"
repeat_region 226696..227037
/note="Ty3 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007158"
repeat_region 227096..227293
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007156"
repeat_region complement(227851..228178)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007151"
gene 228331..228404
/locus_tag="YNCO0005W"
/db_xref="GeneID:854101"
tRNA 228331..228404
/locus_tag="YNCO0005W"
/product="tRNA-Asn"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Asparagine tRNA (tRNA-Asn), predicted by
tRNAscan-SE analysis"
/db_xref="GeneID:854101"
/db_xref="SGD:S000006675"
gene <228614..>229834
/gene="PSH1"
/locus_tag="YOL054W"
/db_xref="GeneID:854102"
mRNA <228614..>229834
/gene="PSH1"
/locus_tag="YOL054W"
/product="ubiquitin-protein ligase PSH1"
/transcript_id="NM_001183309.1"
/db_xref="GeneID:854102"
CDS 228614..229834
/gene="PSH1"
/locus_tag="YOL054W"
/experiment="EXISTENCE:direct assay:GO:0000775 chromosome,
centromeric region [PMID:21070970]"
/experiment="EXISTENCE:direct assay:GO:0004842
ubiquitin-protein transferase activity
[PMID:21070971|PMID:21070970]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:21070971|PMID:21070970]"
/experiment="EXISTENCE:mutant phenotype:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:21070970|PMID:21070971]"
/note="E3 ubiquitin ligase targeting centromere-binding
protein Cse4p; mediates polyubiquitination and degradation
of histone H3 variant Cse4p; ubiquitination of Cse4p may
be antagonized by Scm3p; interacts with the FACT complex
preventing mislocalization of Cse4p to euchromatin
independent of Slx5p; formyl-methionine/N-recognin
component of N-end rule pathway mediating destruction of
formylated cytosolic proteins elevated in response to
starvation or stationary phase entry"
/codon_start=1
/product="ubiquitin-protein ligase PSH1"
/protein_id="NP_014587.1"
/db_xref="GeneID:854102"
/db_xref="SGD:S000005415"
/translation="MGDELHNRLLHQNDGTKDAILYKIIESLVCSICHDYMFVPMMTP
CGHNYCYGCLNTWFASNTQKELACPQCRSDITTIPALNTTLQQYLSFILEKLRDQNDE
SFKKLLTTKTKEENDYKNDKEKDTLFDKVFKNSALAVADDSDDGITRCSNCHWELDPD
EVEDGNVCPHCNARIRNYAGGRDEFDEEEYSEGELDEIRESMRRRRENRFASTNPFAN
RDDVSSEDDDSSEEEPMREHIPLGRWARSHNRSIAVDAVDDEDDEEEDEEEEEEMDSD
LKDFIEDDEDDEDEDGSRRNLVLSALKNRHVIITDDEEEEQRRHATEEEDRDSDFYEH
NDDGFVSGDSLDEDQKEVTRIQSSSDSEDRSLSYSGSSDVKDNNDDNTEELDDPQPKR
QKRFRVVLGDSDDE"
gene <230085..>231272
/gene="AIM39"
/locus_tag="YOL053W"
/db_xref="GeneID:854103"
mRNA <230085..>231272
/gene="AIM39"
/locus_tag="YOL053W"
/product="Aim39p"
/transcript_id="NM_001183308.1"
/db_xref="GeneID:854103"
CDS 230085..231272
/gene="AIM39"
/locus_tag="YOL053W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:35563734]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/note="hypothetical protein; null mutant displays elevated
frequency of mitochondrial genome loss; localizes to
mitochondria, cytosol and to the peroxisome in a
Pex5p-dependent manner in glucose"
/codon_start=1
/product="Aim39p"
/protein_id="NP_014588.1"
/db_xref="GeneID:854103"
/db_xref="SGD:S000005414"
/translation="MWGLCKKHFPSNKIQVQERNKALKPKKSGSEHKTKQLFPVFNCK
KKEKGVMIRFAILRNANTSLLSARSICLFTQAPTYCHVRLNTLNKSITTKRNSLTESK
RHVHDGKHFFTTPHQQQQTKLGEIEEGHSPNIKGEDLRSIGQAITHQRNKRRKQIWSA
IFGGIFGVILGYSLIYRVIYLKEQSFLPLFPSSKIRKLSTRDLKKVDVNQVQKLSKLR
VLEILSGHDMIKEQYGVPLLDKDGNSPTLNEFSMWCEDQDPCVTGIVMEPDDKRDSSH
TWYRIPFVCKWRITHRPISIRGTIDDLLNRIGLETADLFEIISPERVYGSFKYEYPLQ
GDSHALHLWFHGEIELDDDSLIVYNGKYHVDVKLQEIDLFRREKNGQLIQYVLYKNEA
GDK"
gene complement(<231570..>231755)
/gene="DDR2"
/locus_tag="YOL052C-A"
/gene_synonym="YOL053C-A"
/db_xref="GeneID:854104"
mRNA complement(<231570..>231755)
/gene="DDR2"
/locus_tag="YOL052C-A"
/gene_synonym="YOL053C-A"
/product="Ddr2p"
/transcript_id="NM_001183307.1"
/db_xref="GeneID:854104"
CDS complement(231570..231755)
/gene="DDR2"
/locus_tag="YOL052C-A"
/gene_synonym="YOL053C-A"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:expression pattern:GO:0006974 DNA
damage response [PMID:9473471]"
/experiment="EXISTENCE:expression pattern:GO:0009267
cellular response to starvation [PMID:9473471]"
/experiment="EXISTENCE:expression pattern:GO:0034605
cellular response to heat [PMID:9473471]"
/experiment="EXISTENCE:expression pattern:GO:0070301
cellular response to hydrogen peroxide [PMID:9473471]"
/note="Multi-stress response protein; expression is
activated by a variety of xenobiotic agents and
environmental or physiological stresses; DDR2 has a
paralog, HOR7, that arose from the whole genome
duplication"
/codon_start=1
/product="Ddr2p"
/protein_id="NP_014589.1"
/db_xref="GeneID:854104"
/db_xref="SGD:S000005413"
/translation="MKVSQVFISAISVFGLATSVNAQNASNTTSNAAPALHAQNGQLL
NAGVVGAAVGGALAFLI"
gene complement(<232446..>233636)
/gene="SPE2"
/locus_tag="YOL052C"
/db_xref="GeneID:854105"
mRNA complement(<232446..>233636)
/gene="SPE2"
/locus_tag="YOL052C"
/product="adenosylmethionine decarboxylase SPE2"
/transcript_id="NM_001183306.1"
/db_xref="GeneID:854105"
CDS complement(232446..233636)
/gene="SPE2"
/locus_tag="YOL052C"
/EC_number="4.1.1.50"
/experiment="EXISTENCE:direct assay:GO:0004014
adenosylmethionine decarboxylase activity [PMID:2266128]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0006597
spermine biosynthetic process [PMID:7929015]"
/experiment="EXISTENCE:mutant phenotype:GO:0008295
spermidine biosynthetic process [PMID:7929015]"
/experiment="EXISTENCE:mutant phenotype:GO:0015940
pantothenate biosynthetic process [PMID:11154694]"
/note="S-adenosylmethionine decarboxylase; required for
the biosynthesis of spermidine and spermine; cells lacking
Spe2p require spermine or spermidine for growth in the
presence of oxygen but not when grown anaerobically"
/codon_start=1
/product="adenosylmethionine decarboxylase SPE2"
/protein_id="NP_014590.1"
/db_xref="GeneID:854105"
/db_xref="SGD:S000005412"
/translation="MTVTIKELTNHNYIDHELSATLDSTDAFEGPEKLLEIWFFPHKK
SITTEKTLRNIGMDRWIEILKLVKCEVLSMKKTKELDAFLLSESSLFVFDHKLTMKTC
GTTTTLFCLEKLFQIVEQELSWAFRTTQGGKYKPFKVFYSRRCFLFPCKQAAIHQNWA
DEVDYLNKFFDNGKSYSVGRNDKSNHWNLYVTETDRSTPKGKEYIEDDDETFEVLMTE
LDPECASKFVCGPEASTTALVEPNEDKGHNLGYQMTKNTRLDEIYVNSAQDSDLSFHH
DAFAFTPCGYSSNMILAEKYYYTLHVTPEKGWSYASFESNIPVFDISQGKQDNLDVLL
HILNVFQPREFSMTFFTKNYQNQSFQKLLSINESLPDYIKLDKIVYDLDDYHLFYMKL
QKKI"
gene 234346..234546
/gene="SNR81"
/locus_tag="YNCO0006W"
/db_xref="GeneID:9164973"
ncRNA 234346..234546
/ncRNA_class="snoRNA"
/gene="SNR81"
/locus_tag="YNCO0006W"
/product="SNR81"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:15306656]"
/experiment="EXISTENCE:curator inference:GO:0031429 box
H/ACA snoRNP complex [PMID:15306656]"
/experiment="EXISTENCE:direct assay:GO:0030565 snRNA
pseudouridylation guide activity [PMID:15962000]"
/experiment="EXISTENCE:direct assay:GO:0031120 snRNA
pseudouridine synthesis [PMID:15962000]"
/experiment="EXISTENCE:mutant phenotype:GO:0030559 rRNA
pseudouridylation guide activity [PMID:15306656]"
/experiment="EXISTENCE:mutant phenotype:GO:0031118 rRNA
pseudouridine synthesis [PMID:15306656]"
/experiment="EXISTENCE:mutant phenotype:GO:0031120 snRNA
pseudouridine synthesis [PMID:15962000]"
/note="H/ACA box small nucleolar RNA (snoRNA); guides
pseudouridylation of large subunit (LSU) rRNA at position
U1052, constitutive pseudouridylation of U2 snRNA (LSR1)
at position 42, and stress-induced U2 pseudouridylation at
position 93"
/transcript_id="NR_132256.1"
/db_xref="GeneID:9164973"
/db_xref="SGD:S000081375"
gene <234940..>238185
/gene="GAL11"
/locus_tag="YOL051W"
/gene_synonym="ABE1; MED15; RAR3; SDS4; SPT13"
/db_xref="GeneID:854106"
mRNA <234940..>238185
/gene="GAL11"
/locus_tag="YOL051W"
/gene_synonym="ABE1; MED15; RAR3; SDS4; SPT13"
/product="Gal11p"
/transcript_id="NM_001183305.1"
/db_xref="GeneID:854106"
CDS 234940..238185
/gene="GAL11"
/locus_tag="YOL051W"
/gene_synonym="ABE1; MED15; RAR3; SDS4; SPT13"
/experiment="EXISTENCE:direct assay:GO:0001095 TFIIE-class
transcription factor complex binding [PMID:8790357]"
/experiment="EXISTENCE:direct assay:GO:0001097 TFIIH-class
transcription factor complex binding [PMID:10973956]"
/experiment="EXISTENCE:direct assay:GO:0003713
transcription coactivator activity [PMID:8378310]"
/experiment="EXISTENCE:direct assay:GO:0045944 positive
regulation of transcription by RNA polymerase II
[PMID:8187178]"
/experiment="EXISTENCE:direct assay:GO:0051123 RNA
polymerase II preinitiation complex assembly
[PMID:8378310|PMID:12482986]"
/experiment="EXISTENCE:direct assay:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:20308326]"
/experiment="EXISTENCE:direct assay:GO:0070847 core
mediator complex [PMID:9891034]"
/experiment="EXISTENCE:genetic interaction:GO:0070202
regulation of establishment of protein localization to
chromosome [PMID:23447536]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:11470794]"
/experiment="EXISTENCE:mutant phenotype:GO:0003713
transcription coactivator activity [PMID:15254252]"
/experiment="EXISTENCE:mutant phenotype:GO:0034605
cellular response to heat [PMID:23447536]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:9891034]"
/experiment="EXISTENCE:mutant phenotype:GO:0070202
regulation of establishment of protein localization to
chromosome [PMID:23447536]"
/experiment="EXISTENCE:mutant phenotype:GO:2000219
positive regulation of invasive growth in response to
glucose limitation [PMID:18202364]"
/note="Subunit of the RNA polymerase II mediator complex;
associates with core polymerase subunits to form the RNA
polymerase II holoenzyme; affects transcription by acting
as target of activators and repressors; forms part of the
tail domain of mediator"
/codon_start=1
/product="Gal11p"
/protein_id="NP_014591.1"
/db_xref="GeneID:854106"
/db_xref="SGD:S000005411"
/translation="MSAAPVQDKDTLSNAERAKNVNGLLQVLMDINTLNGGSSDTADK
IRIHAKNFEAALFAKSSSKKEYMDSMNEKVAVMRNTYNTRKNAVTAAAANNNIKPVEQ
HHINNLKNSGNSANNMNVNMNLNPQMFLNQQAQARQQVAQQLRNQQQQQQQQQQQQRR
QLTPQQQQLVNQMKVAPIPKQLLQRIPNIPPNINTWQQVTALAQQKLLTPQDMEAAKE
VYKIHQQLLFKARLQQQQAQAQAQANNNNNGLPQNGNINNNINIPQQQQMQPPNSSAN
NNPLQQQSSQNTVPNVLNQINQIFSPEEQRSLLQEAIETCKNFEKTQLGSTMTEPVKQ
SFIRKYINQKALRKIQALRDVKNNNNANNNGSNLQRAQNVPMNIIQQQQQQNTNNNDT
IATSATPNAAAFSQQQNASSKLYQMQQQQQAQAQAQAQAQAQAQAQAQAQAAQAAQAQ
AQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAHAQHQPSQQPQQAQQQPNPLHGLTPTA
KDVEVIKQLSLDASKTNLRLTDVTNSLSNEEKEKIKMKLKQGQKLFVQVSNFAPQVYI
ITKNENFLKEVFQLRIFVKEILEKCAEGIFVVKLDTVDRLIIKYQKYWESMRIQILRR
QAILRQQQQMANNNGNPGTTSTGNNNNIATQQNMQQSLQQMQHLQQLKMQQQQQQQQQ
QQQQQQQQQQQQQQHIYPSSTPGVANYSAMANAPGNNIPYMNHKNTSSMDFLNSMENT
PKVPVSAAATPSLNKTINGKVNGRTKSNTIPVTSIPSTNKKLSISNAASQQPTPRSAS
NTAKSTPNTNPSPLKTQTKNGTPNPNNMKTVQSPMGAQPSYNSAIIENAFRKEELLLK
DLEIRKLEISSRFKHRQEIFKDSPMDLFMSTLGDCLGIKDEEMLTSCTIPKAVVDHIN
GSGKRKPTKAAQRARDQDSIDISIKDNKLVMKSKFNKSNRSYSIALSNVAAIFKGIGG
NFKDLSTLVHSSSPSTSSNMDVGNPRKRKASVLEISPQDSIASVLSPDSNIMSDSKKI
KVDSPDDPFMTKSGATTSEKQEVTNEAPFLTSGTSSEQFNVWDWNNWTSAT"
gene <238619..>240094
/gene="GSH2"
/locus_tag="YOL049W"
/db_xref="GeneID:854108"
mRNA <238619..>240094
/gene="GSH2"
/locus_tag="YOL049W"
/product="glutathione synthase"
/transcript_id="NM_001183303.1"
/db_xref="GeneID:854108"
CDS 238619..240094
/gene="GSH2"
/locus_tag="YOL049W"
/EC_number="6.3.2.3"
/experiment="EXISTENCE:direct assay:GO:0004363 glutathione
synthase activity [PMID:9512666]"
/experiment="EXISTENCE:mutant phenotype:GO:0006750
glutathione biosynthetic process [PMID:9512666]"
/note="Glutathione synthetase; catalyzes the ATP-dependent
synthesis of glutathione (GSH) from gamma-glutamylcysteine
and glycine; induced by oxidative stress and heat shock"
/codon_start=1
/product="glutathione synthase"
/protein_id="NP_014593.1"
/db_xref="GeneID:854108"
/db_xref="SGD:S000005409"
/translation="MAHYPPSKDQLNELIQEVNQWAITNGLSMYPPKFEENPSNASVS
PVTIYPTPIPRKCFDEAVQIQPVFNELYARITQDMAQPDSYLHKTTEALALSDSEFTG
KLWSLYLATLKSAQYKKQNFRLGIFRSDYLIDKKKGTEQIKQVEFNTVSVSFAGLSEK
VDRLHSYLNRANKYDPKGPIYNDQNMVISDSGYLLSKALAKAVESYKSQQSSSTTSDP
IVAFIVQRNERNVFDQKVLELNLLEKFGTKSVRLTFDDVNDKLFIDDKTGKLFIRDTE
QEIAVVYYRTGYTTTDYTSEKDWEARLFLEKSFAIKAPDLLTQLSGSKKIQQLLTDEG
VLGKYISDAEKKSSLLKTFVKIYPLDDTKLGREGKRLALSEPSKYVLKPQREGGGNNV
YKENIPNFLKGIEERHWDAYILMELIEPELNENNIILRDNKSYNEPIISELGIYGCVL
FNDEQVLSNEFSGSLLRSKFNTSNEGGVAAGFGCLDSIILY"
gene complement(<240204..>241310)
/gene="RRT8"
/locus_tag="YOL048C"
/db_xref="GeneID:854109"
mRNA complement(join(<240204..240947,241026..>241310))
/gene="RRT8"
/locus_tag="YOL048C"
/product="Rrt8p"
/transcript_id="NM_001183302.1"
/db_xref="GeneID:854109"
CDS complement(join(240204..240947,241026..241310))
/gene="RRT8"
/locus_tag="YOL048C"
/experiment="EXISTENCE:direct assay:GO:0005619 ascospore
wall [PMID:23966878]"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:23966878]"
/experiment="EXISTENCE:direct assay:GO:0005811 lipid
droplet [PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0030476
ascospore wall assembly [PMID:23966878]"
/note="Protein involved in spore wall assembly; shares
similarity with Lds1p and Lds2p and a strain mutant for
all 3 genes exhibits reduced dityrosine fluorescence
relative to the single mutants; identified in a screen for
mutants with increased levels of rDNA transcription; green
fluorescent protein (GFP)-fusion protein localizes to
lipid particles; protein abundance increases in response
to DNA replication stress"
/codon_start=1
/product="Rrt8p"
/protein_id="NP_014594.2"
/db_xref="GeneID:854109"
/db_xref="SGD:S000005408"
/translation="MKAGIELISHSQASHATYANSMTLAEKGPQRLKRQFKEHSSSKE
SNVSRWLKIFIRQFDIWFPETIPTMKVRYELLRKNFIKEIFNSRAFIYPFLGFYEVLT
NPVYWKHILLFAVCYALIFVTIAGLFYVTLVPLLVTWAILLLGPLGVILVHIQWILQT
NVLTAFVCRTLVLTHITNQIFDISLVLQDQDEFLNEVKVLPKPQKPHRKIDEPDAVRN
FNTIKGSRIFKIPRLLFRMFFKVSNFTSLTLLSLIPIVGPILANQLMAPKRTFTYLQR
YFLLKGFSKKQAKDFQYEHYASFICFGMSAGLLELIPFFTIVTISSNTVGAAKWCTSL
LKGERKKE"
gene complement(<241614..>242747)
/gene="LDS2"
/locus_tag="YOL047C"
/db_xref="GeneID:854110"
mRNA complement(join(<241614..242441,242505..>242747))
/gene="LDS2"
/locus_tag="YOL047C"
/product="Lds2p"
/transcript_id="NM_001183301.1"
/db_xref="GeneID:854110"
CDS complement(join(241614..242441,242505..242747))
/gene="LDS2"
/locus_tag="YOL047C"
/experiment="EXISTENCE:direct assay:GO:0005619 ascospore
wall [PMID:23966878]"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:23966878]"
/experiment="EXISTENCE:direct assay:GO:0005633 ascus lipid
droplet [PMID:23966878]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005811 lipid
droplet [PMID:24390141]"
/experiment="EXISTENCE:genetic interaction:GO:0030476
ascospore wall assembly [PMID:23966878]"
/note="Protein Involved in spore wall assembly; localizes
to lipid droplets found on or outside of the prospore
membrane; shares similarity with Lds1p and Rrt8p, and a
strain mutant for all 3 genes exhibits reduced dityrosine
fluorescence relative to the single mutants; green
fluorescent protein (GFP)-fusion protein localizes to the
cytoplasm in a punctate pattern"
/codon_start=1
/product="Lds2p"
/protein_id="NP_014595.2"
/db_xref="GeneID:854110"
/db_xref="SGD:S000005407"
/translation="MSTRPQPDWYYHRHPYASTPLAEGEEPQLLPIQDQGNHKKSKIW
MAYKAPIVRWYKNAMLVKDNFWKDLESSHQIIWYPYKGISESVGNSDYLHLFFLIFGY
YLLNLLLIVAFTSILAWSLLVCIYLPFLGLFALPLAYMQTILISTTLCNSMVKGTDFV
LFTRIYGVTFARKGLTELSEACETISFTPFVYRRSHRLGGLFSKRFYLVSLPQFFIFF
FWYIFIAFMFLLLLLVPIVGPITINMLPFSPGMGFYYFEPYFVDVLHLDSRKLSKVYY
KGFAKWLLYSISSGLLESIPILGGLFIGTNAVGASLWIVKEIKDRDQPAVPPSPPAEP
EEPTVGSYAPPIQQSIAHINPP"
gene <243497..>246802
/gene="PSK2"
/locus_tag="YOL045W"
/db_xref="GeneID:854111"
mRNA <243497..>246802
/gene="PSK2"
/locus_tag="YOL045W"
/product="serine/threonine protein kinase PSK2"
/transcript_id="NM_001183299.1"
/db_xref="GeneID:854111"
CDS 243497..246802
/gene="PSK2"
/locus_tag="YOL045W"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004674 protein
serine/threonine kinase activity [PMID:12372297]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:genetic interaction:GO:0045719
negative regulation of glycogen biosynthetic process
[PMID:12372297]"
/experiment="EXISTENCE:genetic interaction:GO:0060917
regulation of (1->6)-beta-D-glucan biosynthetic process
[PMID:17531808]"
/experiment="EXISTENCE:mutant phenotype:GO:0004674 protein
serine/threonine kinase activity [PMID:12372297]"
/experiment="EXISTENCE:mutant phenotype:GO:0045719
negative regulation of glycogen biosynthetic process
[PMID:12372297]"
/experiment="EXISTENCE:mutant phenotype:GO:2000766
negative regulation of cytoplasmic translation
[PMID:26310304]"
/note="PAS-domain containing serine/threonine protein
kinase; regulates sugar flux and translation in response
to an unknown metabolite by phosphorylating Ugp1p and
Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p
(translation); PSK2 has a paralog, PSK1, that arose from
the whole genome duplication"
/codon_start=1
/product="serine/threonine protein kinase PSK2"
/protein_id="NP_014597.1"
/db_xref="GeneID:854111"
/db_xref="SGD:S000005405"
/translation="MTYPVSAAAPADISYSKNTPLVGLSKPPCLYQHASSSVDSFSST
FSDDDRSDLVAVPNESPHAFSYNPISPNSLGVRLTILRRSLEIMVNSPDILHELKKKA
PVIAYPPSLRHTRNLTETATLSASRDPLNGSLISPLVSNMPSPASRPVIQRATSLMVL
PDNDTASKLNPAKSELENLLFLLNLALENNSFERASDLHMLSLLNIKKINFDSDIQKS
ETLKKVLLDSLAEPFFENYKKFPHKDLGSKSQYNEYEEKHDDIVSLADIKPQQDYSRI
LHPFTSAKNSGPEAIFTCSQQYPWNFKAANDLACLTFGISKNVIKALTLLDLIHTDSR
NFVLEKIMNAEDDNQEIVFTGETIPIVQPNSTSNNNVPNLIWASLWAKRKNGLLVCVF
EKTPCDYIDVMLNLRDFSVDSIIDTTHFLENFDKKKQQESTSPMTEKKTVKFANEIHD
IGSVSHSLSKLIDDVRFGKVFSADDDLLPLSIRVANHVNEERYFTLNCLSENIPCAVT
TSVLENEIKLKIHSLPYQAGLFIVDSHTLSLLSFNKSVAKNMFGLRLHELAGSSVTKL
VPSLADMISYINKTYPMLNITLPENKGLVLTEHFFRKIEAEMHHDKDSFYTSIGLDGC
HKDGNLIKVDVQLRVLNTNAVLLWITHSRDVVIENYTTVPSQLPMLKENEIDVVGSRG
SSSASSKKSSEKIPVNTLKAMADLSISSAETISNSDDEVDLNQVNEKLRETSCGKVRG
IESNDNNNYDDDMTMVDDPELKHKIELTKMYTQDKSKFVKDDNFKVDEKFIMRIIEPI
NGEEIKKETNELDKRNSTLKATYLTTPEANIGSQKRIKKFSDFTILQVMGEGAYGKVN
LCIHNREHYIVVIKMIFKERILVDTWVRDRKLGTIPSEIQIMATLNKNSQENILKLLD
FFEDDDYYYIETPVHGETGSIDLFDVIEFKKDMVEHEAKLVFKQVVASIKHLHDQGIV
HRDIKDENVIVDSHGFVKLIDFGSAAYIKSGPFDVFVGTMDYAAPEVLGGSSYKGKPQ
DIWALGVLLYTIIYKENPYYNIDEILEGELRFDKSEHVSEECISLIKRILTREVDKRP
TIDEIYEDKWLKI"
gene <247150..>248301
/gene="PEX15"
/locus_tag="YOL044W"
/gene_synonym="PAS21"
/db_xref="GeneID:854113"
mRNA <247150..>248301
/gene="PEX15"
/locus_tag="YOL044W"
/gene_synonym="PAS21"
/product="Pex15p"
/transcript_id="NM_001183298.1"
/db_xref="GeneID:854113"
CDS 247150..248301
/gene="PEX15"
/locus_tag="YOL044W"
/gene_synonym="PAS21"
/experiment="EXISTENCE:direct assay:GO:0005778 peroxisomal
membrane [PMID:16911527|PMID:9405362]"
/experiment="EXISTENCE:direct assay:GO:0016562 protein
import into peroxisome matrix, receptor recycling
[PMID:16007078]"
/experiment="EXISTENCE:direct assay:GO:0043335 protein
unfolding [PMID:16007078]"
/experiment="EXISTENCE:direct assay:GO:0140318 protein
transporter activity [PMID:16007078]"
/experiment="EXISTENCE:direct assay:GO:1904949 ATPase
complex [PMID:16007078]"
/experiment="EXISTENCE:genetic interaction:GO:0043495
protein-membrane adaptor activity [PMID:12808025]"
/experiment="EXISTENCE:mutant phenotype:GO:0016562 protein
import into peroxisome matrix, receptor recycling
[PMID:16007078]"
/experiment="EXISTENCE:mutant phenotype:GO:0043495
protein-membrane adaptor activity [PMID:12808025]"
/experiment="EXISTENCE:physical interaction:GO:0043495
protein-membrane adaptor activity
[PMID:16911527|PMID:12808025|PMID:16007078]"
/note="Tail-anchored type II integral peroxisomal membrane
protein; required for peroxisome biogenesis; cells lacking
Pex15p mislocalize peroxisomal matrix proteins to cytosol;
overexpression results in impaired peroxisome assembly"
/codon_start=1
/product="Pex15p"
/protein_id="NP_014598.1"
/db_xref="GeneID:854113"
/db_xref="SGD:S000005404"
/translation="MAASEIMNNLPMHSLDSSLRDLLNDDLFIESDESTKSVNDQRSE
VFQECVNLFIKRDIKDCLEKMSEVGFIDITVFKSNPMILDLFVSACDIMPSFTKLGLT
LQSEILNIFTLDTPQCIETRKIILGDLSKLLVINKFFRCCIKVIQFNLTDHTEQEEKT
LELESIMSDFIFVYITKMRTTIDVVGLQELIEIFIFQVKVKLHHKKPSPNMYWALCKT
LPKLSPTLKGLYLSKDVSIEDAILNSIDNKIQKDKAKSKGKQRGVKQKIHHFHEPMLH
NSSEEQVKVEDAFNQRTSTDSRLQSTGTAPRKKNNDITVLAGSFWAVLKHHFTRSVLN
KNGLLLTGLLLLLCLKKYKSLMAIFKHVPAAFHTVYPQIVGLLKLLASI"
gene complement(<248392..>249534)
/gene="NTG2"
/locus_tag="YOL043C"
/gene_synonym="SCR2"
/db_xref="GeneID:854114"
mRNA complement(<248392..>249534)
/gene="NTG2"
/locus_tag="YOL043C"
/gene_synonym="SCR2"
/product="bifunctional N-glycosylase/AP lyase NTG2"
/transcript_id="NM_001183297.1"
/db_xref="GeneID:854114"
CDS complement(248392..249534)
/gene="NTG2"
/locus_tag="YOL043C"
/gene_synonym="SCR2"
/EC_number="4.2.99.18"
/experiment="EXISTENCE:direct assay:GO:0000703 oxidized
pyrimidine nucleobase lesion DNA N-glycosylase activity
[PMID:9020769]"
/experiment="EXISTENCE:direct assay:GO:0003906
DNA-(apurinic or apyrimidinic site) endonuclease activity
[PMID:14500818]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10471279|PMID:19029246]"
/experiment="EXISTENCE:direct assay:GO:0006284
base-excision repair [PMID:14500818]"
/experiment="EXISTENCE:direct assay:GO:0006285
base-excision repair, AP site formation [PMID:9826748]"
/note="DNA N-glycosylase and apurinic/apyrimidinic (AP)
lyase; involved in base excision repair, localizes to the
nucleus; sumoylated; NTG2 has a paralog, NTG1, that arose
from the whole genome duplication"
/codon_start=1
/product="bifunctional N-glycosylase/AP lyase NTG2"
/protein_id="NP_014599.1"
/db_xref="GeneID:854114"
/db_xref="SGD:S000005403"
/translation="MREESRSRKRKHIPVDIEEVEVRSKYFKKNERTVELVKENKINK
DLQNYGGVNIDWIKALKPIEYFEWIESRTCDDPRTWGRPITKEEMINDSGAKVPESFL
PIYNRVRLMRSKVKTPVDAMGCSMIPVLVSNKCGIPSEKVDPKNFRLQFLIGTMLSAQ
TRDERMAQAALNITEYCLNTLKIAEGITLDGLLKIDEPVLANLIRCVSFYTRKANFIK
RTAQLLVDNFDSDIPYDIEGILSLPGVGPKMGYLTLQKGWGLIAGICVDVHVHRLCKM
WNWVDPIKCKTAEHTRKELQVWLPHSLWYEINTVLVGFGQLICMARGKRCDLCLANDV
CNARNEKLIESSKFHQLEDKEDIEKVYSHWLDTVTNGITTERHKKK"
gene <249825..>250916
/gene="NGL1"
/locus_tag="YOL042W"
/db_xref="GeneID:854115"
mRNA <249825..>250916
/gene="NGL1"
/locus_tag="YOL042W"
/product="RNA exonuclease"
/transcript_id="NM_001183296.1"
/db_xref="GeneID:854115"
CDS 249825..250916
/gene="NGL1"
/locus_tag="YOL042W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239|PMID:14562095]"
/note="Putative endonuclease; has a domain similar to a
magnesium-dependent endonuclease motif in mRNA deadenylase
Ccr4p; the authentic, non-tagged protein is detected in
highly purified mitochondria in high-throughput studies"
/codon_start=1
/product="RNA exonuclease"
/protein_id="NP_014600.1"
/db_xref="GeneID:854115"
/db_xref="SGD:S000005402"
/translation="MFTRRFIPVVQSTKQNIGKYVRKDARFTLLTYNMLSPSYMWPQV
YTYVAEPYKNWSYRHRLLEKELLNTFKADIMCLQEMTARDYEDYWHDSIGVDVNYGSK
FISKTPPKYWKKPVKDMDGVSIFYNLAKFDFISSSGIYLNQLLNVFNQRELKYLYNKK
VTLTDGASNVIGEDSLLDVLKGKNQVCLFVSLRHKETGTIFVVLNTHLYWKYDEVKLT
QCMIIMRELSKIIKQLLPGDVKGQERVKILFTGDLNSTRDSLVVNFLQGQIVSHGDLN
LINPMRPYLDRCVYDDIPKDYFVHTCYSGKLKGIFDYVWYHDSDFLLTKILTGNEVSD
ELLASNQLGLPNENHPSDHIPLLTEFKIL"
gene complement(<251267..>252646)
/gene="NOP12"
/locus_tag="YOL041C"
/db_xref="GeneID:854116"
mRNA complement(<251267..>252646)
/gene="NOP12"
/locus_tag="YOL041C"
/product="rRNA-processing protein NOP12"
/transcript_id="NM_001183295.1"
/db_xref="GeneID:854116"
CDS complement(251267..252646)
/gene="NOP12"
/locus_tag="YOL041C"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:11452019]"
/experiment="EXISTENCE:direct assay:GO:0030684 preribosome
[PMID:17922018]"
/experiment="EXISTENCE:mutant phenotype:GO:0000463
maturation of LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11452019]"
/note="Nucleolar protein involved in pre-25S rRNA
processing; also involved in biogenesis of large 60S
ribosomal subunit; contains an RNA recognition motif
(RRM); binds to Ebp2; similar to Nop13p and Nsr1p"
/codon_start=1
/product="rRNA-processing protein NOP12"
/protein_id="NP_014601.1"
/db_xref="GeneID:854116"
/db_xref="SGD:S000005401"
/translation="MSSAIDNLFGNIDEKKIESSVDKLFSSSCGPINKLEVKSKTRTV
LPDSKKRERAAEADQEEKEASKPDVSDEQTEEVALPKVKKAKKSKRNDEDEDLEARYY
AKLLNEEAEAEDDKPTVTKTDETSVPLTSAAKKVDFKEDELEKAERTVFIGNILSTVI
TSKKVYKEFKKLFGTNPIAETEESGNEKEEESSKKSDNNEFAIESIRFRSISFDEALP
RKVAFVQQKFHKSRDTINAYIVYKNKSAVRKICSNLNAVVFQDHHLRVDSVAHPAPHD
KKRSIFVGNLDFEEIEESLWKHFEPCGDIEYVRIIRDSKTNMGKGFAYVQFKDLQSVN
KALLLNEKPMKSQKQEDENTKKPTKKARKLRVSRCKNMKKGTTIGTGLDRNGLTDSQR
TRAGRAKKILGKADRATLGQEITIEGLRAKKGEGSTHLKKKKQRSATGRVTKRSIAFK
KAQAEKSKK"
gene complement(<253149..>253577)
/gene="RPS15"
/locus_tag="YOL040C"
/gene_synonym="RPS21"
/db_xref="GeneID:854117"
mRNA complement(<253149..>253577)
/gene="RPS15"
/locus_tag="YOL040C"
/gene_synonym="RPS21"
/product="40S ribosomal protein uS19 RPS15"
/transcript_id="NM_001183294.1"
/db_xref="GeneID:854117"
CDS complement(253149..253577)
/gene="RPS15"
/locus_tag="YOL040C"
/gene_synonym="RPS21"
/experiment="EXISTENCE:mutant phenotype:GO:0000054
ribosomal subunit export from nucleus
[PMID:16246728|PMID:15167894]"
/note="Protein component of the small (40S) ribosomal
subunit; homologous to mammalian ribosomal protein S15 and
bacterial S19"
/codon_start=1
/product="40S ribosomal protein uS19 RPS15"
/protein_id="NP_014602.1"
/db_xref="GeneID:854117"
/db_xref="SGD:S000005400"
/translation="MSQAVNAKKRVFKTHSYRGVDLEKLLEMSTEDFVKLAPARVRRR
FARGMTSKPAGFMKKLRAAKLAAPENEKPAPVRTHMRNMIIVPEMIGSVVGIYNGKAF
NQVEIRPEMLGHYLGEFSITYTPVRHGRAGATTSRFIPLK"
gene <254297..>254617
/gene="RPP2A"
/locus_tag="YOL039W"
/gene_synonym="RPL44; RPLA2"
/db_xref="GeneID:854118"
mRNA <254297..>254617
/gene="RPP2A"
/locus_tag="YOL039W"
/gene_synonym="RPL44; RPLA2"
/product="ribosomal protein P2 RPP2A"
/transcript_id="NM_001183293.1"
/db_xref="GeneID:854118"
CDS 254297..254617
/gene="RPP2A"
/locus_tag="YOL039W"
/gene_synonym="RPL44; RPLA2"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:22096102]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:22096102]"
/experiment="EXISTENCE:genetic interaction:GO:0002181
cytoplasmic translation [PMID:1601875]"
/experiment="EXISTENCE:mutant phenotype:GO:0002181
cytoplasmic translation [PMID:9559554]"
/note="Ribosomal protein P2 alpha; a component of the
ribosomal stalk, which is involved in the interaction
between translational elongation factors and the ribosome;
free (non-ribosomal) P2 stimulates the phosphorylation of
the eIF2 alpha subunit (Sui2p) by Gcn2p; regulates the
accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm"
/codon_start=1
/product="ribosomal protein P2 RPP2A"
/protein_id="NP_014603.1"
/db_xref="GeneID:854118"
/db_xref="SGD:S000005399"
/translation="MKYLAAYLLLNAAGNTPDATKIKAILESVGIEIEDEKVSSVLSA
LEGKSVDELITEGNEKLAAVPAAGPASAGGAAAASGDAAAEEEKEEEAAEESDDDMGF
GLFD"
gene complement(<254926..>255021)
/locus_tag="YOL038C-A"
/db_xref="GeneID:1466476"
mRNA complement(<254926..>255021)
/locus_tag="YOL038C-A"
/product="uncharacterized protein"
/transcript_id="NM_001184641.1"
/db_xref="GeneID:1466476"
CDS complement(254926..255021)
/locus_tag="YOL038C-A"
/note="hypothetical protein; identified by SAGE analysis"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878164.1"
/db_xref="GeneID:1466476"
/db_xref="SGD:S000028812"
/translation="MKYMGSFLRKAATTNLFNSIKKRKVQNRAMS"
gene <255337..>256101
/gene="PRE6"
/locus_tag="YOL038W"
/db_xref="GeneID:854119"
mRNA <255337..>256101
/gene="PRE6"
/locus_tag="YOL038W"
/product="proteasome core particle subunit alpha 4"
/transcript_id="NM_001183292.1"
/db_xref="GeneID:854119"
CDS 255337..256101
/gene="PRE6"
/locus_tag="YOL038W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:18504300]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16806052]"
/experiment="EXISTENCE:direct assay:GO:0010499 proteasomal
ubiquitin-independent protein catabolic process
[PMID:19162040]"
/experiment="EXISTENCE:direct assay:GO:0019773 proteasome
core complex, alpha-subunit complex [PMID:9087403]"
/experiment="EXISTENCE:direct assay:GO:0034515 proteasome
storage granule [PMID:18504300]"
/experiment="EXISTENCE:direct assay:GO:0042175 nuclear
outer membrane-endoplasmic reticulum membrane network
[PMID:9799224]"
/experiment="EXISTENCE:direct assay:GO:0043161
proteasome-mediated ubiquitin-dependent protein catabolic
process [PMID:19029916|PMID:11545745]"
/note="Alpha 4 subunit of the 20S proteasome; may replace
alpha 3 subunit (Pre9p) under stress conditions to create
a more active proteasomal isoform; GFP-fusion protein
relocates from cytosol to the mitochondrial surface upon
oxidative stress"
/codon_start=1
/product="proteasome core particle subunit alpha 4"
/protein_id="NP_014604.1"
/db_xref="GeneID:854119"
/db_xref="SGD:S000005398"
/translation="MSGYDRALSIFSPDGHIFQVEYALEAVKRGTCAVGVKGKNCVVL
GCERRSTLKLQDTRITPSKVSKIDSHVVLSFSGLNADSRILIEKARVEAQSHRLTLED
PVTVEYLTRYVAGVQQRYTQSGGVRPFGVSTLIAGFDPRDDEPKLYQTEPSGIYSSWS
AQTIGRNSKTVREFLEKNYDRKEPPATVEECVKLTVRSLLEVVQTGAKNIEITVVKPD
SDIVALSSEEINQYVTQIEQEKQEQQEQDKKKKSNH"
gene <256745..>259030
/locus_tag="YOL036W"
/db_xref="GeneID:854120"
mRNA <256745..>259030
/locus_tag="YOL036W"
/product="uncharacterized protein"
/transcript_id="NM_001183290.1"
/db_xref="GeneID:854120"
CDS 256745..259030
/locus_tag="YOL036W"
/note="hypothetical protein; potential Cdc28p substrate;
YOL036W has a paralog, YIR016W, that arose from the whole
genome duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014606.1"
/db_xref="GeneID:854120"
/db_xref="SGD:S000005396"
/translation="MEHQDSSPPRFRNSGSNRVTVYNGTTLPTMPKSATPTSSSTTVT
THLQNIKEEETNDDELTQVDRSSPRVLGRISSTSSSSSNIDLRDNLDMLHEIEKSNTN
ISLSAPNLHEELGVLSDKGNSKEELALLPPLPHTGEMEITPQFDINEAIFERDDISHS
SRLEPDDVLTKLANSTRDATGEDQGFVVMTHGHDASTNDDSQLSATILDNQTSFDLSK
ALEMTSHSNISNIINSSGSEGRRSRTPVSNSTLKPNLSSPESAEREANTTSSSSTSDH
GATMQYDPKKIITPIPVLPSSVREQQQNNAPLRERSRSNSSALASTLRDTIISGLPQN
INSVERKLSRKSNRSRKNTVTFEDRLQKLPPLSTQISNQYAKVAPAENNIALHFHNLP
TPVSNTQTPVTFQSESGLTGGEKKMPFLRRASSALLRKTSAKNCSNLTRTNTPTLSTS
STFESDLNARQPMLIRRSSTIDNKLPRRQLSCSKLYSRLNSDSKFANSSRASEEVLVS
TPNDTEHVYRKTSLGSKIKRGFTRILSDSNNSKEILTLSPKSMVTTGPTELSFSSLST
VGGHPTTPVSKENDRVSIDGVSTFNRASTSLPESSTDDISPLREEGKINVPKRTSSRK
ILSKNSSKKNVLPEQQTKPSEIYLDKEALQSFVPVLSVTEGTHRINRSSLQTQSTIGL
CITNLRNKEGMKLNAKEYVEILAQQQRKEDERYAVLERKFASCRWCSDKDLQYLKKKR
ISMNKIWSDYVRFYRGKLNNP"
gene 259489..259578
/gene="SNR50"
/locus_tag="YNCO0007W"
/db_xref="GeneID:9164975"
ncRNA 259489..259578
/ncRNA_class="snoRNA"
/gene="SNR50"
/locus_tag="YNCO0007W"
/product="SNR50"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:10024243]"
/experiment="EXISTENCE:curator inference:GO:0031428 box
C/D methylation guide snoRNP complex [PMID:10024243]"
/experiment="EXISTENCE:mutant phenotype:GO:0030562 rRNA
2'-O-ribose methylation guide activity [PMID:10024243]"
/experiment="EXISTENCE:mutant phenotype:GO:0031167 rRNA
methylation [PMID:10024243]"
/note="C/D box small nucleolar RNA (snoRNA); guides
2'-O-methylation of large subunit (LSU) rRNA at position
G867"
/transcript_id="NR_132257.1"
/db_xref="GeneID:9164975"
/db_xref="SGD:S000006441"
gene <259923..>263204
/gene="SMC5"
/locus_tag="YOL034W"
/db_xref="GeneID:854123"
mRNA <259923..>263204
/gene="SMC5"
/locus_tag="YOL034W"
/product="DNA repair ATPase SMC5"
/transcript_id="NM_001183288.1"
/db_xref="GeneID:854123"
CDS 259923..263204
/gene="SMC5"
/locus_tag="YOL034W"
/experiment="EXISTENCE:direct assay:GO:0003684 damaged DNA
binding [PMID:16793545]"
/experiment="EXISTENCE:direct assay:GO:0003697
single-stranded DNA binding [PMID:21293191]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11927594]"
/experiment="EXISTENCE:direct assay:GO:0006281 DNA repair
[PMID:11927594]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:21293191]"
/experiment="EXISTENCE:direct assay:GO:0019789 SUMO
transferase activity [PMID:15738391]"
/experiment="EXISTENCE:direct assay:GO:0030915 Smc5-Smc6
complex [PMID:15738391]"
/experiment="EXISTENCE:mutant phenotype:GO:0006281 DNA
repair [PMID:15738391]"
/experiment="EXISTENCE:mutant phenotype:GO:0051304
chromosome separation [PMID:15793567]"
/experiment="EXISTENCE:mutant phenotype:GO:0071139
resolution of DNA recombination intermediates
[PMID:20159973]"
/experiment="EXISTENCE:physical interaction:GO:0000725
recombinational repair [PMID:19995966]"
/note="Subunit of the SMC5-SMC6 complex; the SMC5-SMC6
complex plays a key role in the removal of X-shaped DNA
structures that arise between sister chromatids during DNA
replication and repair; binds single-stranded DNA and has
ATPase activity; supports nucleolar function; S. pombe
homolog forms a heterodimer with S. pombe Rad18p that is
involved in DNA repair"
/codon_start=1
/product="DNA repair ATPase SMC5"
/protein_id="NP_014608.1"
/db_xref="GeneID:854123"
/db_xref="SGD:S000005394"
/translation="MTSLIDLGRYVERTHHGEDTEPRSKRVKIAKPDLSSFQPGSIIK
IRLQDFVTYTLTEFNLSPSLNMIIGPNGSGKSTFVCAVCLGLAGKPEYIGRSKKVEDF
IKNGQDVSKIEITLKNSPNVTDIEYIDARDETIKITRIITRSKRRSDYLINDYQVSES
VVKTLVAQLNIQLDNLCQFLSQERVEEFARLKSVKLLVETIRSIDASLLDVLDELREL
QGNEQSLQKDLDFKKAKIVHLRQESDKLRKSVESLRDFQNKKGEIELHSQLLPYVKVK
DHKEKLNIYKEEYERAKANLRAILKDKKPFANTKKTLENQVEELTEKCSLKTDEFLKA
KEKINEIFEKLNTIRDEVIKKKNQNEYYRGRTKKLQATIISTKEDFLRSQEILAQTHL
PEKSVFEDIDIKRKEIINKEGEIRDLISEIDAKANAINHEMRSIQRQAESKTKSLTTT
DKIGILNQDQDLKEVRDAVLMVREHPEMKDKILEPPIMTVSAINAQFAAYLAQCVDYN
TSKALTVVDSDSYKLFANPILDKFKVNLRELSSADTTPPVPAETVRDLGFEGYLSDFI
TGDKRVMKMLCQTSKIHTIPVSRRELTPAQIKKLITPRPNGKILFKRIIHGNRLVDIK
QSAYGSKQVFPTDVSIKQTNFYQGSIMSNEQKIRIENEIINLKNEYNDRKSTLDALSN
QKSGYRHELSELASKNDDINREAHQLNEIRKKYTMRKSTIETLREKLDQLKREARKDV
SQKIKDIDDQIQQLLLKQRHLLSKMASSMKSLKNCQKELISTQILQFEAQNMDVSMND
VIGFFNEREADLKSQYEDKKKFVKEMRDTPEFQSWMREIRSYDQDTKEKLNKVAEKYE
EEGNFNLSFVQDVLDKLESEIAMVNHDESAVTILDQVTAELRELEHTVPQQSKDLETI
KAKLKEDHAVLEPKLDDIVSKISARFARLFNNVGSAGAVRLEKPKDYAEWKIEIMVKF
RDNAPLKKLDSHTQSGGERAVSTVLYMIALQEFTSAPFRVVDEINQGMDSRNERIVHK
AMVENACAENTSQYFLITPKLLTGLHYHEKMRIHCVMAGSWIPNPSEDPKMIHFGETS
NYSFD"
gene <263475..>265085
/gene="MSE1"
/locus_tag="YOL033W"
/db_xref="GeneID:854124"
mRNA <263475..>265085
/gene="MSE1"
/locus_tag="YOL033W"
/product="glutamate--tRNA ligase MSE1"
/transcript_id="NM_001183287.1"
/db_xref="GeneID:854124"
CDS 263475..265085
/gene="MSE1"
/locus_tag="YOL033W"
/EC_number="6.1.1.17"
/experiment="EXISTENCE:curator inference:GO:0070149
mitochondrial glutamyl-tRNA aminoacylation [PMID:7607232]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:mutant phenotype:GO:0005739
mitochondrion [PMID:7607232]"
/experiment="EXISTENCE:mutant phenotype:GO:0032543
mitochondrial translation [PMID:7607232]"
/note="Mitochondrial glutamyl-tRNA synthetase; predicted
to be palmitoylated"
/codon_start=1
/product="glutamate--tRNA ligase MSE1"
/protein_id="NP_014609.1"
/db_xref="GeneID:854124"
/db_xref="SGD:S000005393"
/translation="MIMLRIPTRSYCSPSKLIKGVGLSPLKKSLLSKKIKEDIHPSLP
VRTRFAPSPTGFLHLGSLRTALYNYLLARNTNGQFLLRLEDTDQKRLIEGAEENIYEI
LKWCNINYDETPIKQSERKLIYDKYVKILLSSGKAYRCFCSKERLNDLRHSAMELKPP
SMASYDRCCAHLGEEEIKSKLAQGIPFTVRFKSPERYPTFTDLLHGQINLQPQVNFND
KRYDDLILVKSDKLPTYHLANVVDDHLMGITHVIRGEEWLPSTPKHIALYNAFGWACP
KFIHIPLLTTVGDKKLSKRKGDMSISDLKRQGVLPEALINFCALFGWSPPRDLASKKH
ECFSMEELETIFNLNGLTKGNAKVDDKKLWFFNKHFLQKRILNPSTLRELVDDIMPSL
ESIYNTSTISREKVAKILLNCGGSLSRINDFHDEFYYFFEKPKYNDNDAVTKFLSKNE
SRHIAHLLKKLGQFQEGTDAQEVESMVETMYYENGFSRKVTYQAMRFALAGCHPGAKI
AAMIDILGIKESNKRLSEGLQFLQREKK"
gene <265429..>266169
/gene="OPI10"
/locus_tag="YOL032W"
/db_xref="GeneID:854125"
mRNA <265429..>266169
/gene="OPI10"
/locus_tag="YOL032W"
/product="Opi10p"
/transcript_id="NM_001183286.1"
/db_xref="GeneID:854125"
CDS 265429..266169
/gene="OPI10"
/locus_tag="YOL032W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0006020
inositol metabolic process [PMID:16582425]"
/note="Protein with a possible role in phospholipid
biosynthesis; null mutant displays an inositol-excreting
phenotype that is suppressed by exogenous choline; protein
abundance increases in response to DNA replication stress"
/codon_start=1
/product="Opi10p"
/protein_id="NP_014610.1"
/db_xref="GeneID:854125"
/db_xref="SGD:S000005392"
/translation="MFAAIASGNPLQLSVEVPNSNGLQHTIVLSRTKPKLYSHITLFI
LPNVTFPQDYIATVYFKLSPQEEFKLFGYLSSEKPSAIFKVQIPSSKKDAGDTSDGLG
EIDMDVDDGSGAADPFTDTNGSSSNNISELIIGISIEPREQGMMKLEEWKASMNAEAQ
KNNSLILSRPNLGIIRNITTAGQLAQVYPSLTQELAAKIVQHAYNYLSGFLDAQGNVP
IKRFDTWWDKFRNRLANDGTFLDEVTKN"
gene complement(<266264..>267529)
/gene="SIL1"
/locus_tag="YOL031C"
/gene_synonym="SLS1"
/db_xref="GeneID:854126"
mRNA complement(<266264..>267529)
/gene="SIL1"
/locus_tag="YOL031C"
/gene_synonym="SLS1"
/product="Sil1p"
/transcript_id="NM_001183285.1"
/db_xref="GeneID:854126"
CDS complement(266264..267529)
/gene="SIL1"
/locus_tag="YOL031C"
/gene_synonym="SLS1"
/experiment="EXISTENCE:direct assay:GO:0000774
adenyl-nucleotide exchange factor activity
[PMID:10958688]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0006616
SRP-dependent cotranslational protein targeting to
membrane, translocation [PMID:10958688]"
/note="Nucleotide exchange factor for the ER lumenal Hsp70
chaperone Kar2p; required for protein translocation into
the endoplasmic reticulum (ER); homolog of Yarrowia
lipolytica SLS1; GrpE-like protein"
/codon_start=1
/product="Sil1p"
/protein_id="NP_014611.1"
/db_xref="GeneID:854126"
/db_xref="SGD:S000005391"
/translation="MVRILPIILSALSSKLVASTILHSSIHSVPSGGEIISAEDLKEL
EISGNSICVDNRCYPKIFEPRHDWQPILPGQELPGGLDIRINMDTGLKEAKLNDEKNV
GDNGSHELIVSSEDMKASPGDYEFSSDFKEMRNIIDSNPTLSSQDIARLEDSFDRIME
FAHDYKHGYKIITHEFALLANLSLNENLPLTLRELSTRVITSCLRNNPPVVEFINESF
PNFKSKIMAALSNLNDSNHRSSNILIKRYLSILNELPVTSEDLPIYSTVVLQNVYERN
NKDKQLQIKVLELISKILKADMYENDDTNLILFKRNAENWSSNLQEWANEFQEMVQNK
SIDELHTRTFFDTLYNLKKIFKSDITINKGFLNWLAQQCKARQSNLDNGLQERDTEQD
SFDKKLIDSRHLIFGNPMAHRIKNFRDEL"
gene <268187..>269641
/gene="GAS5"
/locus_tag="YOL030W"
/db_xref="GeneID:854127"
mRNA <268187..>269641
/gene="GAS5"
/locus_tag="YOL030W"
/product="1,3-beta-glucanosyltransferase"
/transcript_id="NM_001183284.1"
/db_xref="GeneID:854127"
CDS 268187..269641
/gene="GAS5"
/locus_tag="YOL030W"
/experiment="EXISTENCE:curator inference:GO:0006074
(1->3)-beta-D-glucan metabolic process
[PMID:17397106|PMID:10769178]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:15781460|PMID:10383953]"
/experiment="EXISTENCE:direct assay:GO:0042124
1,3-beta-glucanosyltransferase activity [PMID:17397106]"
/note="1,3-beta-glucanosyltransferase; has similarity to
Gas1p; localizes to the cell wall"
/codon_start=1
/product="1,3-beta-glucanosyltransferase"
/protein_id="NP_014612.1"
/db_xref="GeneID:854127"
/db_xref="SGD:S000005390"
/translation="MLLRSLTSAFVLSAGLAQAASSSNSSTPSIEIKGNAFFNSESGE
RFYIRGVDYQPGGSSNLTDPLADASVCDRDVPVLKDLGINTVRVYTVDNSQDHSHCMK
LLQENGIYLILDVNTPTSAISRYDPACSYNADYLQNVFATIDTFADYDNVLGFFAGNE
VINSVNTTNTATYVKAVVRDMKKYIKARKYRQIPVGYSAADIVANRQLAAEYFNCGDE
ADARIDMFGVNDYSWCGESSFVVSGYSTKMKLYQDYSVPVFLSEFGCNQVKSSRPFTE
IEAIYSTQMSSVFSGGLVYEYSNETNNYGLVQIDGDKVTKLTDFENLKNEYSKVSNPE
GNGGYSTSNNYSTCPDYEKGVWEANNTLPAMPSAASAYFTSGAGSPMGTGIATQQSCD
AKDDDDEEDDDTSSSSSSSSSSSSSASSSSESSSSTSKASSSSPSASETSLLKSAASA
TSSSQSSSKSKGAAGIIEIPLIFRALAELYNLVL"
gene complement(<269815..>270420)
/locus_tag="YOL029C"
/db_xref="GeneID:854128"
mRNA complement(<269815..>270420)
/locus_tag="YOL029C"
/product="uncharacterized protein"
/transcript_id="NM_001183283.1"
/db_xref="GeneID:854128"
CDS complement(269815..270420)
/locus_tag="YOL029C"
/note="hypothetical protein; separated from neighboring
gene, YAP7, by frame shift and corresponds to C-terminal
part of YAP7 orthologs in other species; identified as
interacting with Hsc82p and Hsp82p in high-throughput
two-hybrid screens"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014613.1"
/db_xref="GeneID:854128"
/db_xref="SGD:S000005389"
/translation="MKPVTCCNQKNNIMPSLVPVCCSEKKIESDAKKSISKCCGDKEI
YDSENRPITKEDGSWIPGSCKQCRSDPHSRNFCQSLSNKCSSSSFSSNSALSPDLNEQ
QTDVNYNSIKLPEICSCKNAQMNAASDAKRYLPISYTYQKIRQHMQKNKSIQEQLNPE
DSTSISSALENIASGLHVRGQKVELQSIKDALHKMDKNVLE"
gene complement(<270633..>271370)
/gene="YAP7"
/locus_tag="YOL028C"
/db_xref="GeneID:854129"
mRNA complement(<270633..>271370)
/gene="YAP7"
/locus_tag="YOL028C"
/product="Yap7p"
/transcript_id="NM_001183282.1"
/db_xref="GeneID:854129"
CDS complement(270633..271370)
/gene="YAP7"
/locus_tag="YOL028C"
/experiment="EXISTENCE:curator inference:GO:0005634
nucleus [PMID:20802496]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:20802496]"
/note="Transcriptional repressor of nitric oxide oxidase;
basic leucine zipper (bZIP) transcription factor;
neighboring gene, YOL029C, created by frame-shift at
position 708, corresponds to C-terminal part of YAP7
orthologs in other species; YAP7 has a paralog, YAP5, that
arose from the whole genome duplication"
/codon_start=1
/product="Yap7p"
/protein_id="NP_014614.1"
/db_xref="GeneID:854129"
/db_xref="SGD:S000005388"
/translation="MRQRRSVVAVSVKPKGFKLGHKQGSMSTTSPPPSSPDGNVSTSG
PSAIKLSKNWELPQRLKPGRKPKSKRGDASANNDGSSKIKKVQTSNQKDQMTTKDHEN
EGAKGHEGKSDDEGNGSGDENGVDSVEKRRRQNRDAQRAYRERRTTRIQVLEEKVEML
HNLVDDWQRKYKLLESEFSDTKENLQKSIALNNELQKALPLIVNTPFQQQPENPPDNP
ISILEMVENFKPIGAVSLKKGKLKAHC"
gene complement(<272003..>273724)
/gene="MDM38"
/locus_tag="YOL027C"
/gene_synonym="MKH1"
/db_xref="GeneID:854130"
mRNA complement(<272003..>273724)
/gene="MDM38"
/locus_tag="YOL027C"
/gene_synonym="MKH1"
/product="ribosome-binding protein MDM38"
/transcript_id="NM_001183281.1"
/db_xref="GeneID:854130"
CDS complement(272003..273724)
/gene="MDM38"
/locus_tag="YOL027C"
/gene_synonym="MKH1"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:14562095|PMID:16823961|PMID:14576278|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane
[PMID:15138253|PMID:16476776]"
/experiment="EXISTENCE:direct assay:GO:0097177
mitochondrial ribosome binding [PMID:20427570]"
/experiment="EXISTENCE:genetic interaction:GO:0006813
potassium ion transport [PMID:20197279]"
/experiment="EXISTENCE:genetic interaction:GO:0070131
positive regulation of mitochondrial translation
[PMID:20427570]"
/experiment="EXISTENCE:genetic interaction:GO:1902600
proton transmembrane transport [PMID:20197279]"
/experiment="EXISTENCE:mutant phenotype:GO:0006813
potassium ion transport [PMID:15904662]"
/experiment="EXISTENCE:mutant phenotype:GO:0030007
intracellular potassium ion homeostasis [PMID:15138253]"
/experiment="EXISTENCE:mutant phenotype:GO:0032979 protein
insertion into mitochondrial inner membrane from matrix
[PMID:16476776]"
/note="Membrane-associated mitochondrial ribosome
receptor; forms a complex with Mba1p to facilitate
recruitment of mRNA-specific translational activators to
ribosomes; ribosome-associated protein involved in the
insertion of newly synthesized proteins into the
mitochondrial inner membrane; role in protein export and
K+/H+ exchange; localizes to the inner mitochondrial
membrane; human ortholog Letm1 implicated in
Wolf-Hirschhorn syndrome"
/codon_start=1
/product="ribosome-binding protein MDM38"
/protein_id="NP_014615.1"
/db_xref="GeneID:854130"
/db_xref="SGD:S000005387"
/translation="MLNFASRASCVTRRQASLYFVKNQGPRLIASTIPSCHWPLRAQG
VQPNYPLSLRFYSTDKSKSVTKPVAPTSTDAPAKPKETLMVKVKHALKHYANGTKLLG
YEIKVSTKLLIKFAQGYELSRRERNQLRRTMGDVFRLIPFSAFLIIPFAELFLPFALK
LFPNLLPSTYESGKDKQAKRNKLIEIRKKTSEFLHETLEESNLITYNTIENAEKKQKF
LNFFRKLYSAKEGKIMTFQHDEISAIAQMFKNDSVLDNLSRPQLAAMSKFMSLRPFGN
DNMLRYQIRSKLKDIMNDDKTIDYEGVESLSQEELYQACVSRGMKAYGVSKEDLVDNL
KVWLELRLRQKIPSVLMVLSSTFTFGGLPKENYSKAFSPLAEKKETKSKYDDLLDLYY
DGILQVLSSIPDPVYNVAKLDVSESKSSAAETEAEKQVAEKKIKTEEKPEETAIPKEE
ATAKESVIATTASAVTPKLVVVNEKAETAKTEEISQEKENAEPTDSAEATEAEEKKTS
DDNEFKLNVLKEQEELIKKEEEEAKQRASREHVPDDINLDEEEEAKSVPPIPADQAAK
TFVIKKD"
gene complement(<274012..>274353)
/gene="MIM1"
/locus_tag="YOL026C"
/gene_synonym="TOM13"
/db_xref="GeneID:854131"
mRNA complement(<274012..>274353)
/gene="MIM1"
/locus_tag="YOL026C"
/gene_synonym="TOM13"
/product="Mim1p"
/transcript_id="NM_001183280.1"
/db_xref="GeneID:854131"
CDS complement(274012..274353)
/gene="MIM1"
/locus_tag="YOL026C"
/gene_synonym="TOM13"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane
[PMID:15326197|PMID:15608614]"
/experiment="EXISTENCE:mutant phenotype:GO:0045040 protein
insertion into mitochondrial outer membrane
[PMID:21825073|PMID:21825074]"
/experiment="EXISTENCE:mutant phenotype:GO:0070096
mitochondrial outer membrane translocase complex assembly
[PMID:17974559|PMID:15608614]"
/experiment="EXISTENCE:physical interaction:GO:0140595 MIM
complex [PMID:22467864]"
/note="Mitochondrial protein required for outer membrane
protein import; cooperates with Tom70p to import the
subset of proteins with multiple alpha-helical
transmembrane segments, including Ugo1p, Tom20p, and
others; forms a complex with Mim2p in the outer membrane
that functions as cation-selective channel for
translocation of positively charged precursor segments;
also has a role in assembly of Tom20p into the TOM
complex"
/codon_start=1
/product="Mim1p"
/protein_id="NP_014616.1"
/db_xref="GeneID:854131"
/db_xref="SGD:S000005386"
/translation="MTEVVGFWESVSDDESEDKDCMEVQNTVSADESPLVQSLVSFVG
SCSINLLLPFLNGMMLGFGELFAHELCWRFNWFNHRNKGYKVYPESRKIAALKEISSP
GTRGRVASKFL"
gene 274673..274773
/locus_tag="YNCO0008W"
/db_xref="GeneID:854132"
tRNA join(274673..274709,274729..274773)
/locus_tag="YNCO0008W"
/product="tRNA-Ser"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:854132"
/db_xref="SGD:S000006733"
gene <274957..>276939
/gene="LAG2"
/locus_tag="YOL025W"
/gene_synonym="ECM36"
/db_xref="GeneID:854133"
mRNA <274957..>276939
/gene="LAG2"
/locus_tag="YOL025W"
/gene_synonym="ECM36"
/product="Lag2p"
/transcript_id="NM_001183279.1"
/db_xref="GeneID:854133"
CDS 274957..276939
/gene="LAG2"
/locus_tag="YOL025W"
/gene_synonym="ECM36"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:genetic interaction:GO:0031397
negative regulation of protein ubiquitination
[PMID:19942853]"
/experiment="EXISTENCE:mutant phenotype:GO:0031397
negative regulation of protein ubiquitination
[PMID:19763088]"
/note="Protein that negatively regulates the SCF
E3-ubiquitin ligase; regulates by interacting with and
preventing neddyation of the cullin subunit, Cdc53p;
longevity determinant that is preferentially expressed in
young cells; similar to mammalian Cand1"
/codon_start=1
/product="Lag2p"
/protein_id="NP_014617.1"
/db_xref="GeneID:854133"
/db_xref="SGD:S000005385"
/translation="MSLHISKLIEQYRSTKDNDLKYMLLRQNFKINDIEDELAPLVNE
LLLPVLVEEQDMEILNLVSFQVLPDLVLSMISDPAAAQLGWVISLICDPLLNQSMIHA
NRSFVLIETLRNVLQKIENSPHLDYHQPVNSSLEFISKFIVEMKRHMCDVDAAQLSHS
LSESNMLIYIESLNLLLKFSFFSDAASPSVMVTLPFDILNDVFTIAQDYSATNTNESI
DRITEKLLLTSTQLTHPVDLENLCPKMKYNTLAAVSRIWYKFGPIVDKLFTNRLLPVL
FPPQMGEECNVEDVLEIVHNFHPYFSIRRLKDNRPLLSDSTISQLREGLFGMLSILND
SLTRTQNENDHGSDNLIDSDDGFGSDNDPEQQAYLDELVSEGYDENMYDGDTDDEDAD
DINVEKNDEATKDITETNKILLIFSELHYPQEERFSELLVELQTKIAINTSLIDKILS
KETTELPTHNGEIADLNEILNEVKGNKPIRKNVIFCTLAHTLSLQSGSELSVLQLSIE
VIDHLLVKNHSNNITRGEQFQLIKLILPHLKTNKSFIDTLKAGNFTQKIDEGVTLRTM
ILSLLLQLFPLDYSMLGEILPTIARYSVRDKDLGVRDLSFQLLDQILRTYYNYLIGID
WEWYKDDFYQVLQETCIKKDINTNLLLQFPPYLPHD"
gene <277086..>277604
/locus_tag="YOL024W"
/db_xref="GeneID:854134"
mRNA <277086..>277604
/locus_tag="YOL024W"
/product="uncharacterized protein"
/transcript_id="NM_001183278.1"
/db_xref="GeneID:854134"
CDS 277086..277604
/locus_tag="YOL024W"
/note="hypothetical protein; predicted to have
thiol-disulfide oxidoreductase active site; YOL024W has a
paralog, IGD1, that arose from the whole genome
duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014618.1"
/db_xref="GeneID:854134"
/db_xref="SGD:S000005384"
/translation="MSKLSSYPHAADFINMEEPPKSKEFFDDLCAVPNLLKRRFPNSR
RSTHYCEALNYSRKKLPVVLSKMTLQELRHNMSTFFLQEKDQINIYDTCKVIDMGDRV
LLETMPPQPRDLFEKLHASKTNLVVQTAALDEPLLTVKAELQSSSFPQKSSLFLYEDY
KKFIYQQLDMFS"
rep_origin 277530..277778
/note="ARS1511; Autonomously Replicating Sequence;
replication capacity dependent upon Sum1p"
/db_xref="SGD:S000118488"
gene <278056..>280086
/gene="IFM1"
/locus_tag="YOL023W"
/db_xref="GeneID:854135"
mRNA <278056..>280086
/gene="IFM1"
/locus_tag="YOL023W"
/product="translation initiation factor 2"
/transcript_id="NM_001183277.1"
/db_xref="GeneID:854135"
CDS 278056..280086
/gene="IFM1"
/locus_tag="YOL023W"
/experiment="EXISTENCE:direct assay:GO:0000049 tRNA
binding [PMID:12729623]"
/experiment="EXISTENCE:direct assay:GO:0003743 translation
initiation factor activity [PMID:15935987]"
/experiment="EXISTENCE:direct assay:GO:0003924 GTPase
activity [PMID:12729623]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:24769239|PMID:16823961|PMID:11914276|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0005739
mitochondrion [PMID:1935960]"
/experiment="EXISTENCE:mutant phenotype:GO:0032543
mitochondrial translation [PMID:1935960]"
/note="Mitochondrial translation initiation factor 2"
/codon_start=1
/product="translation initiation factor 2"
/protein_id="NP_014619.1"
/db_xref="GeneID:854135"
/db_xref="SGD:S000005383"
/translation="MLRRHGLFWLKTCPRLNVLLNQSIPIPHLLHSRDICQQRWYAKG
KRRNQISKKELKPLNFSIPNYISVNKLANLLNCRVERLIKDLTALGFENITTTYILSK
EYVELILQEYNFALPNLSTSTNLDNVYDELKSPVNPKLLTKRAPVVTIMGHVDHGKTT
IIDYLRKSSVVAQEHGGITQHIGAFQITAPKSGKKITFLDTPGHAAFLKMRERGANIT
DIIVLVVSVEDSLMPQTLEAIKHAKNSGNEMIIAITKIDRIPQPKEREKKIEKVINDL
IVQGIPVEKIGGDVQVIPISAKTGENMDLLEESIVLLSEVMDIRAENSPKTIAEGWII
ESQVKKQVGNVATVLVKKGTLQKGKILICGNTFCKIKNLIDDKGIPILKATPSYATEV
LGWKDVPHVGDEVIQVKSEAIAKKFISKRQDLIEVQKNSSIVEKLNEERALAKEQHLN
KELEHENTVQEHEQNTGPKLINYIIKCDVSGSAEAVSESISSLGNDEVRCNVISSSVG
IPTESDLKMAQITESTILCFNLGNLPSEVINNRAGIKIKQYNVIYKLIEDVTETLTEN
LKPIFEKKIVSTVDVRETFDFRLKKKIIRIAGCKVNNGVIKKNSLVQVVRGPNEDVIF
DGKISTLKHNKDDVAEVSKGHECGITFESGFEGFKPGDKILVYENVRVPRYL"
gene complement(<280272..>281498)
/gene="TSR4"
/locus_tag="YOL022C"
/db_xref="GeneID:854136"
mRNA complement(<280272..>281498)
/gene="TSR4"
/locus_tag="YOL022C"
/product="small subunit rRNA maturation protein TSR4"
/transcript_id="NM_001183276.2"
/db_xref="GeneID:854136"
CDS complement(280272..281498)
/gene="TSR4"
/locus_tag="YOL022C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:31182640|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0051082 unfolded
protein binding [PMID:31182640|PMID:31062022]"
/experiment="EXISTENCE:genetic interaction:GO:0042274
ribosomal small subunit biogenesis [PMID:31062022]"
/experiment="EXISTENCE:mutant phenotype:GO:0030490
maturation of SSU-rRNA [PMID:19806183]"
/experiment="EXISTENCE:mutant phenotype:GO:0042274
ribosomal small subunit biogenesis
[PMID:31182640|PMID:31062022]"
/experiment="EXISTENCE:physical interaction:GO:0051082
unfolded protein binding [PMID:31182640]"
/note="Cytoplasmic assembly chaperone for ribosomal
protein Rps2p; co-translationally associates with the
N-terminal extension of Rps2p to facilitate expression,
solubility and ribosome assembly; released prior to the
nuclear import of Rps2p; required for correct processing
of 20S pre-rRNA at site D to generate mature 18S rRNA;
essential gene in the S288C background but not in CEN.PK2;
homologous to human PDCD2L and PDCD2"
/codon_start=1
/product="small subunit rRNA maturation protein TSR4"
/protein_id="NP_014620.1"
/db_xref="GeneID:854136"
/db_xref="SGD:S000005382"
/translation="MSKIEELPPSDTDDHSYSSKPGDVFLAFVDAPVKETDDILVEDS
FIGGEPKWLHPDSEPPAELLKCGACKSADNMKLLLQAFSPLDDEQMSAIQQRLGINNM
SYINPQDDRVLYVFLCTECQRKGNSVRCIRGVKKNKNVDSLSEKMASTSLEKDFQINP
FDLSNNSDSKCNAFSSNPFGGANANPFGADSINSNISQSKDEGKKKESATVSAKTARK
LHDLQKDKEYDGNKCFKSCLLYVEEETFKNKKPAHLQLPKNLKIDKEALDLTGDEDLE
KDPIKLDPRTEKLSKFLDDDTFQKFQEVVGYNPLQVLRYDLGGKPLLYAETKVDILST
VPRPGYNPSSQRIFEMQLMPKMIFDLEEVVSVDNGMEWGTILVFTDVENYMPEFDEHG
VGYVEECVKVQWESRT"
gene 282164..282234
/gene="SUF17"
/locus_tag="YNCO0009W"
/db_xref="GeneID:854137"
tRNA 282164..282234
/gene="SUF17"
/locus_tag="YNCO0009W"
/product="tRNA-Gly"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0030371 translation
repressor activity [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043555 regulation
of translation in response to stress [PMID:27609601]"
/note="Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE
analysis; can mutate to suppress +1 frameshift mutations
in glycine codons"
/db_xref="GeneID:854137"
/db_xref="SGD:S000006587"
gene complement(<282421..>285426)
/gene="DIS3"
/locus_tag="YOL021C"
/gene_synonym="MTR17; RRP44"
/db_xref="GeneID:854138"
mRNA complement(<282421..>285426)
/gene="DIS3"
/locus_tag="YOL021C"
/gene_synonym="MTR17; RRP44"
/product="exosome catalytic subunit DIS3"
/transcript_id="NM_001183275.1"
/db_xref="GeneID:854138"
CDS complement(282421..285426)
/gene="DIS3"
/locus_tag="YOL021C"
/gene_synonym="MTR17; RRP44"
/experiment="EXISTENCE:direct assay:GO:0000049 tRNA
binding [PMID:17643380]"
/experiment="EXISTENCE:direct assay:GO:0000175 3'-5'-RNA
exonuclease activity [PMID:17173052|PMID:17643380]"
/experiment="EXISTENCE:direct assay:GO:0000176 nuclear
exosome (RNase complex) [PMID:19046973|PMID:10465791]"
/experiment="EXISTENCE:direct assay:GO:0000177 cytoplasmic
exosome (RNase complex) [PMID:19046973|PMID:10465791]"
/experiment="EXISTENCE:direct assay:GO:0004521 RNA
endonuclease activity
[PMID:19129231|PMID:19060886|PMID:19060898]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:9562621|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0071038
TRAMP-dependent tRNA surveillance pathway
[PMID:15828860|PMID:17643380|PMID:15935758]"
/experiment="EXISTENCE:genetic interaction:GO:0071038
TRAMP-dependent tRNA surveillance pathway [PMID:15145828]"
/experiment="EXISTENCE:mutant phenotype:GO:0000049 tRNA
binding [PMID:17643380]"
/experiment="EXISTENCE:mutant phenotype:GO:0000175
3'-5'-RNA exonuclease activity
[PMID:17173052|PMID:17643380]"
/experiment="EXISTENCE:mutant phenotype:GO:0000467
exonucleolytic trimming to generate mature 3'-end of 5.8S
rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S
rRNA, LSU-rRNA) [PMID:9390555|PMID:10508172]"
/experiment="EXISTENCE:mutant phenotype:GO:0004521 RNA
endonuclease activity [PMID:19129231|PMID:19060886]"
/experiment="EXISTENCE:mutant phenotype:GO:0006397 mRNA
processing [PMID:27385342]"
/experiment="EXISTENCE:mutant phenotype:GO:0016075 rRNA
catabolic process [PMID:10508172]"
/experiment="EXISTENCE:mutant phenotype:GO:0043628
regulatory ncRNA 3'-end processing [PMID:11352936]"
/experiment="EXISTENCE:mutant phenotype:GO:0070651
nonfunctional rRNA decay [PMID:19481524]"
/experiment="EXISTENCE:mutant phenotype:GO:0071028 nuclear
mRNA surveillance [PMID:11030620]"
/experiment="EXISTENCE:mutant phenotype:GO:0071035 nuclear
polyadenylation-dependent rRNA catabolic process
[PMID:18940861|PMID:16431988]"
/experiment="EXISTENCE:mutant phenotype:GO:0071038
TRAMP-dependent tRNA surveillance pathway [PMID:17643380]"
/experiment="EXISTENCE:mutant phenotype:GO:0071039 nuclear
polyadenylation-dependent CUT catabolic process
[PMID:15935759]"
/note="Exosome core complex catalytic subunit; has both
endonuclease and 3'-5' exonuclease activity; involved in
3'-5' RNA processing and degradation in both the nucleus
and the cytoplasm; role in degradation of tRNAs; similar
to E. coli RNase R and to human DIS3, which partially
complements dis3-81 heat sensitivity; mutations in Dis3p
analogous to human mutations implicated in multiple
myeloma impair exosome function; protein abundance
increases under to DNA replication stress"
/codon_start=1
/product="exosome catalytic subunit DIS3"
/protein_id="NP_014621.1"
/db_xref="GeneID:854138"
/db_xref="SGD:S000005381"
/translation="MSVPAIAPRRKRLADGLSVTQKVFVRSRNGGATKIVREHYLRSD
IPCLSRSCTKCPQIVVPDAQNELPKFILSDSPLELSAPIGKHYVVLDTNVVLQAIDLL
ENPNCFFDVIVPQIVLDEVRNKSYPVYTRLRTLCRDSDDHKRFIVFHNEFSEHTFVER
LPNETINDRNDRAIRKTCQWYSEHLKPYDINVVLVTNDRLNREAATKEVESNIITKSL
VQYIELLPNADDIRDSIPQMDSFDKDLERDTFSDFTFPEYYSTARVMGGLKNGVLYQG
NIQISEYNFLEGSVSLPRFSKPVLIVGQKNLNRAFNGDQVIVELLPQSEWKAPSSIVL
DSEHFDVNDNPDIEAGDDDDNNESSSNTTVISDKQRRLLAKDAMIAQRSKKIQPTAKV
VYIQRRSWRQYVGQLAPSSVDPQSSSTQNVFVILMDKCLPKVRIRTRRAAELLDKRIV
ISIDSWPTTHKYPLGHFVRDLGTIESAQAETEALLLEHDVEYRPFSKKVLECLPAEGH
DWKAPTKLDDPEAVSKDPLLTKRKDLRDKLICSIDPPGCVDIDDALHAKKLPNGNWEV
GVHIADVTHFVKPGTALDAEGAARGTSVYLVDKRIDMLPMLLGTDLCSLKPYVDRFAF
SVIWELDDSANIVNVNFMKSVIRSREAFSYEQAQLRIDDKTQNDELTMGMRALLKLSV
KLKQKRLEAGALNLASPEVKVHMDSETSDPNEVEIKKLLATNSLVEEFMLLANISVAR
KIYDAFPQTAMLRRHAAPPSTNFEILNEMLNTRKNMSISLESSKALADSLDRCVDPED
PYFNTLVRIMSTRCMMAAQYFYSGAYSYPDFRHYGLAVDIYTHFTSPIRRYCDVVAHR
QLAGAIGYEPLSLTHRDKNKMDMICRNINRKHRNAQFAGRASIEYYVGQVMRNNESTE
TGYVIKVFNNGIVVLVPKFGVEGLIRLDNLTEDPNSAAFDEVEYKLTFVPTNSDKPRD
VYVFDKVEVQVRSVMDPITSKRKAELLLK"
gene <286172..>287950
/gene="TAT2"
/locus_tag="YOL020W"
/gene_synonym="HPG2; LTG3; SAB2; SCM2; TAP2"
/db_xref="GeneID:854139"
mRNA <286172..>287950
/gene="TAT2"
/locus_tag="YOL020W"
/gene_synonym="HPG2; LTG3; SAB2; SCM2; TAP2"
/product="aromatic amino acid transmembrane transporter
TAT2"
/transcript_id="NM_001183274.1"
/db_xref="GeneID:854139"
CDS 286172..287950
/gene="TAT2"
/locus_tag="YOL020W"
/gene_synonym="HPG2; LTG3; SAB2; SCM2; TAP2"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:12810702|PMID:30660361]"
/experiment="EXISTENCE:direct assay:GO:0015173 aromatic
amino acid transmembrane transporter activity
[PMID:10654085]"
/experiment="EXISTENCE:direct assay:GO:0015801 aromatic
amino acid transport [PMID:10654085]"
/experiment="EXISTENCE:direct assay:GO:0032126 eisosome
[PMID:35031272]"
/experiment="EXISTENCE:direct assay:GO:0044853 plasma
membrane raft [PMID:40601413]"
/experiment="EXISTENCE:mutant phenotype:GO:0005300
high-affinity tryptophan transmembrane transporter
activity [PMID:35031272|PMID:7523855]"
/experiment="EXISTENCE:mutant phenotype:GO:0015827
tryptophan transport [PMID:35031272|PMID:7523855]"
/note="High affinity tryptophan and tyrosine permease;
overexpression confers FK506 and FTY720 resistance"
/codon_start=1
/product="aromatic amino acid transmembrane transporter
TAT2"
/protein_id="NP_014622.1"
/db_xref="GeneID:854139"
/db_xref="SGD:S000005380"
/translation="MTEDFISSVKRSNEELKERKSNFGFVEYKSKQLTSSSSHNSNSS
HHDDDNQHGKRNIFQRCVDSFKSPLDGSFDTSNLKRTLKPRHLIMIAIGGSIGTGLFV
GSGKAIAEGGPLGVVIGWAIAGSQIIGTIHGLGEITVRFPVVGAFANYGTRFLDPSIS
FVVSTIYVLQWFFVLPLEIIAAAMTVQYWNSSIDPVIWVAIFYAVIVSINLFGVRGFG
EAEFAFSTIKAITVCGFIILCVVLICGGGPDHEFIGAKYWHDPGCLANGFPGVLSVLV
VASYSLGGIEMTCLASGETDPKGLPSAIKQVFWRILFFFLISLTLVGFLVPYTNQNLL
GGSSVDNSPFVIAIKLHHIKALPSIVNAVILISVLSVGNSCIFASSRTLCSMAHQGLI
PWWFGYIDRAGRPLVGIMANSLFGLLAFLVKSGSMSEVFNWLMAIAGLATCIVWLSIN
LSHIRFRLAMKAQGKSLDELEFVSAVGIWGSAYSALINCLILIAQFYCSLWPIGGWTS
GKERAKIFFQNYLCALIMLFIFIVHKIYYKCQTGKWWGVKALKDIDLETDRKDIDIEI
VKQEIAEKKMYLDSRPWYVRQFHFWC"
gene 288192..288280
/gene="SUP3"
/locus_tag="YNCO0010W"
/db_xref="GeneID:854140"
tRNA join(288192..288230,288245..288280)
/gene="SUP3"
/locus_tag="YNCO0010W"
/product="tRNA-Tyr"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Tyrosine tRNA (tRNA-Tyr), predicted by tRNAscan-SE
analysis; can mutate to suppress ochre nonsense mutations"
/db_xref="GeneID:854140"
/db_xref="SGD:S000006785"
gene <288420..>288572
/locus_tag="YOL019W-A"
/db_xref="GeneID:1466477"
mRNA <288420..>288572
/locus_tag="YOL019W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184622.1"
/db_xref="GeneID:1466477"
CDS 288420..288572
/locus_tag="YOL019W-A"
/note="hypothetical protein; identified by gene-trapping,
microarray-based expression analysis, and genome-wide
homology searching"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878165.1"
/db_xref="GeneID:1466477"
/db_xref="SGD:S000028707"
/translation="MSNTFVAVEFSWLYAISLILPCETIRVAWAPKRAYHGTSEEKRR
LAPADI"
gene <288898..>290553
/gene="TOS7"
/locus_tag="YOL019W"
/db_xref="GeneID:854141"
mRNA <288898..>290553
/gene="TOS7"
/locus_tag="YOL019W"
/product="Tos7p"
/transcript_id="NM_001183273.1"
/db_xref="GeneID:854141"
CDS 288898..290553
/gene="TOS7"
/locus_tag="YOL019W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0000328 fungal-type
vacuole lumen [PMID:33002606]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:33002606|PMID:15282802]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="Protein involved in secretion and cell wall
organization; localizes to plasma membrane microdomains
called MCCs (membrane compartment occupied by Can1);
GFP-fusion protein localizes to the cell periphery and
vacuole; Sur7/PalI family transmembrane domain (TMD)
containing protein; TOS7 has a paralog, DCV1, that arose
from the whole genome duplication"
/codon_start=1
/product="Tos7p"
/protein_id="NP_014623.1"
/db_xref="GeneID:854141"
/db_xref="SGD:S000005379"
/translation="MKKNSSVVFFLVGLSQFVTMAFLIIGSITAPIFKQIGYSKYDEI
TYGTFGYCKEGSCSKASYNYHPDELSDSDSNWKLNSNARSILGKIIFITPIAAGLNFL
GFLCTIMSVLLINVLSSDRVGSASAIMFFVNLTFSTLGFLSASLICIVVFLLFYPHVT
WCSWVLIPGAALSLLVIPLIFSAYSRSSGSRDDDETEELEEKGMLLNDPYLSSKSGRF
DIDADSEANLRGDSRTNLLGDNFKNGTNITVVPDIISHNQDPKLSNITTSTTSDISTF
DKEAKDMENSNGSGLNEEEDDGMAYDKRRSTSTYSVIESESGLKNGSVSNNYVRNNGS
NTSNNINYKVPLGKTEISSSASLASSDYSQREVIPHRNPSRLLNDIMETSFNEPNDSH
INSMSSYNDKDSTLTSISQRGVNPEVYNQMPRETAAGPANIRPYAGQPHPAPLVYPQQ
RLQPQQQQPQQQYHQYNLYQRTTPAGPDPSNVILQSNPYFNVAPNQVPQHRNPVPGVG
FAPNPLPNQSPITQGYKPAYKRRMQNKNLPRATTSLNNPYGFR"
gene complement(<290881..>292074)
/gene="TLG2"
/locus_tag="YOL018C"
/db_xref="GeneID:854142"
mRNA complement(<290881..>292074)
/gene="TLG2"
/locus_tag="YOL018C"
/product="t-SNARE syntaxin TLG2"
/transcript_id="NM_001183272.1"
/db_xref="GeneID:854142"
CDS complement(290881..292074)
/gene="TLG2"
/locus_tag="YOL018C"
/experiment="EXISTENCE:direct assay:GO:0005484 SNAP
receptor activity [PMID:11739407]"
/experiment="EXISTENCE:direct assay:GO:0005802 trans-Golgi
network [PMID:9565594|PMID:9427746]"
/experiment="EXISTENCE:direct assay:GO:0006906 vesicle
fusion [PMID:11739407]"
/experiment="EXISTENCE:direct assay:GO:0010008 endosome
membrane [PMID:9565594]"
/experiment="EXISTENCE:genetic interaction:GO:0006673
inositol phosphoceramide metabolic process
[PMID:18753406]"
/experiment="EXISTENCE:genetic interaction:GO:0006675
mannosyl-inositol phosphorylceramide metabolic process
[PMID:18753406]"
/experiment="EXISTENCE:genetic interaction:GO:0006896
Golgi to vacuole transport [PMID:18753406]"
/experiment="EXISTENCE:genetic interaction:GO:0032527
protein exit from endoplasmic reticulum [PMID:18753406]"
/experiment="EXISTENCE:mutant phenotype:GO:0006673
inositol phosphoceramide metabolic process
[PMID:18753406]"
/experiment="EXISTENCE:mutant phenotype:GO:0006675
mannosyl-inositol phosphorylceramide metabolic process
[PMID:18753406]"
/experiment="EXISTENCE:mutant phenotype:GO:0006897
endocytosis [PMID:9427746]"
/experiment="EXISTENCE:mutant phenotype:GO:0009306 protein
secretion [PMID:22753847]"
/experiment="EXISTENCE:mutant phenotype:GO:0032258
cytoplasm to vacuole targeting by the Cvt pathway
[PMID:10545112]"
/experiment="EXISTENCE:physical interaction:GO:0031201
SNARE complex [PMID:10397773]"
/note="Syntaxin-like t-SNARE; forms a complex with Tlg1p
and Vti1p and mediates fusion of endosome-derived vesicles
with the late Golgi; required along with VPS45 for an
early step of the constitutive CVT pathway; interactions
with Vps45 prevents Tlg2p degradation, and facilitates
t-SNARE complex formation; homologous to mammalian SNARE
protein syntaxin 16 (Sx16)"
/codon_start=1
/product="t-SNARE syntaxin TLG2"
/protein_id="NP_014624.1"
/db_xref="GeneID:854142"
/db_xref="SGD:S000005378"
/translation="MFRDRTNLFLSYRRTFPHNITFSSGKAPLGDDQDIEMGTYPMMN
MSHDISARLTDERKNKHENHSDALPPIFIDIAQDVDDYLLEVRRLSEQLAKVYRKNSL
PGFEDKSHDEALIEDLSFKVIQMLQKCYAVMKRLKTIYNSQFVDGKQLSREELIILDN
LQKIYAEKIQTESNKFRVLQNNYLKFLNKDDLKPIRNKASAENTLLLDDEEEEAAREK
REGLDIEDYSKRTLQRQQQLHDTSAEAYLRERDEEITQLARGVLEVSTIFREMQDLVV
DQGTIVDRIDYNLENTVVELKSADKELNKATHYQKRTQKCKVILLLTLCVIALFFFVM
LKPHGGGSGGRNNGSNKYNNDDNKTVNNSHDDGSNTHINDEESNLPSIVEVTESENDA
LDDLL"
gene <292529..>294673
/gene="ESC8"
/locus_tag="YOL017W"
/db_xref="GeneID:854143"
mRNA <292529..>294673
/gene="ESC8"
/locus_tag="YOL017W"
/product="Esc8p"
/transcript_id="NM_001183271.1"
/db_xref="GeneID:854143"
CDS 292529..294673
/gene="ESC8"
/locus_tag="YOL017W"
/experiment="EXISTENCE:mutant phenotype:GO:0007062 sister
chromatid cohesion [PMID:27185881]"
/experiment="EXISTENCE:mutant phenotype:GO:0031507
heterochromatin formation [PMID:12399377]"
/experiment="EXISTENCE:physical interaction:GO:0005634
nucleus [PMID:12399377]"
/note="Protein involved in telomeric and mating-type locus
silencing; interacts with Sir2p and also interacts with
Gal11p, which is a component of the RNA pol II mediator
complex; ESC8 has a paralog, IOC3, that arose from the
whole genome duplication"
/codon_start=1
/product="Esc8p"
/protein_id="NP_014625.1"
/db_xref="GeneID:854143"
/db_xref="SGD:S000005377"
/translation="MTEIIDLDLVDDFIKKPMVKQQKNQSSKPRVKRRGQLTFDDFRN
IKIVEEPVVLSHNSSIDESLDAATQNTKKREKYEGTCDEEMKTKEMEANMASKYSNIK
THSNDTNKVESISEHTTSNNRPLNTLNWSPNIPLRYSDFAKFMSDETVTESNWAPPLC
TPLPYAGDVMKILSFIVKFKWVFSDDLLNLSFQDVEIGLELKVAGHSAKNIRICQDKM
NLLFCSLLRLLFCSEKRADNQTHRNFTLKRFLSLKNPYGKLVGKLRSLIQEWGLPKEW
RGNSDILSTLNFNGGGLLTMEPLDRIILLRCMIDWNCSYSALFHNEIQRLTHLKGDTG
FNHQTFHASRFAMCGANNILDSCEVLCSLMSQKLENRKKRKPSDKGKLSKINSQMKFL
KGVRKSLSEKVTTDRLRAAVKINEEWGEYFANEFTHTPIDDPTVDEIYKLRTSEFMIA
RIPRVGDFYLPPFWIGNECSSVNTSYSFNDMSTYLNYFVKFKEEGTKILPAKTAQNEN
KCQLKLIYRNTPACIRNLQFNDVHFAEVPHWFEVAGDSNSLSNFIEYLESLSSLTEND
TDDTKKGIDNLIEFLKIFSIFINETIQRITAAPTGSTEGRHLRTSSQRRTTVHYSSDV
NGDVSEESENEVDIDVSDDYDSEYLSEENTLTRKGEDRTDKSFGKRELHNGAKDCDRN
CDDIEIFSEPVRQLQDNSREKRSLRRNARKGL"
gene complement(<294777..>296120)
/gene="CMK2"
/locus_tag="YOL016C"
/db_xref="GeneID:854144"
mRNA complement(<294777..>296120)
/gene="CMK2"
/locus_tag="YOL016C"
/product="calmodulin-dependent protein kinase CMK2"
/transcript_id="NM_001183270.1"
/db_xref="GeneID:854144"
CDS complement(294777..296120)
/gene="CMK2"
/locus_tag="YOL016C"
/EC_number="2.7.11.17"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0004683
calcium/calmodulin-dependent protein kinase activity
[PMID:2061341]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006468 protein
phosphorylation [PMID:2061341|PMID:16319894]"
/note="Calmodulin-dependent protein kinase; negative
feedback controller of calcium/calcineurin signalling
pathway, also has additional Crz1-independent role in
promoting calcium tolerance; amino acid sequence similar
to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that
arose from the whole genome duplication"
/codon_start=1
/product="calmodulin-dependent protein kinase CMK2"
/protein_id="NP_014626.1"
/db_xref="GeneID:854144"
/db_xref="SGD:S000005376"
/translation="MPKESEVINSEFHVDVQDPERLNGHPVAKFINKLSGQPESYVNR
TNYIFGRTLGAGSFGVVRQARKLSTNEDVAIKILLKKALQGNNVQLQMLYEELSILQK
LSHPNIVSFKDWFESKDKFYIVTQLATGGELFDRILSRGKFTEVDAVEIIVQILGAVE
YMHSKNVVHRDLKPENVLYVDKSENSPLVIADFGIAKQLKGEEDLIYKAAGSLGYVAP
EVLTQDGHGKPCDIWSIGVITYTLLCGYSPFIAESVEGFMEECTASRYPVTFHMPYWD
NISIDVKRFILKALRLNPADRPTATELLDDPWITSKRVETSNILPDVKKGFSLRKKLR
DAIEIVKLNNRIKRLRNMYSLGDDGDNDIEENSLNESLLDGVTHSLDDLRLQSQKKGG
ELTEEQMKLKSALTKDAFVQIVKAATKNKHKVLAGEEEDDSKKTLHDDRESKSED"
gene <297078..>298838
/gene="IRC10"
/locus_tag="YOL015W"
/db_xref="GeneID:854145"
mRNA <297078..>298838
/gene="IRC10"
/locus_tag="YOL015W"
/product="Irc10p"
/transcript_id="NM_001183269.1"
/db_xref="GeneID:854145"
CDS 297078..298838
/gene="IRC10"
/locus_tag="YOL015W"
/experiment="EXISTENCE:direct assay:GO:0070056 prospore
membrane leading edge [PMID:24390141]"
/note="hypothetical protein; subunit of the leading edge
protein (LEP) complex (Ssp1-Ady3-Don1-Irc10) that forms a
ring-like structure at the leading edge of the prospore
membrane during meiosis II; null mutant displays increased
levels of spontaneous Rad52p foci"
/codon_start=1
/product="Irc10p"
/protein_id="NP_014627.1"
/db_xref="GeneID:854145"
/db_xref="SGD:S000005375"
/translation="MFIEYSRLPGFESINISFSRGMLRLAKFTNFATYKQKLEYFRLL
AGSNKYIQRISVADFERHPDEINYIYIILISILQMEECMPVLVLCPTVYWVRFHWPGK
CSVNSLNFTNETLKSAFHAVFTPYFALMKKVLGRIKNNMLLFAEPHANLNNLFVKHFH
DLIYKSVKDEKTGEAILYLRTNVNVPNVFIDDKRAVFHGDGMKIGKFTGKFLCFSFKR
TIRWSKLDSVDSFAVTTVNYRVSVNWEKTPRKTFLSLDSDTKNLHYISKKILNKKGKN
ATTSKTTKSSCTSENVCDDKTFSVEFPLTTSAKTEYLLRSNFSLEKINESNNPTLQEL
TLNRTHRLYRSNFRNEQSTTQRKFEKIGRTVSTDSGNKLLTFPEQKATRDSNPFSIEL
THATVISSDESALKDTTNQAIAEMQRITPAIAKTISRRTANWVCSNPAPDPYGEPSTW
SRILTPNLKIISESSPYYPVHLASPNSTFSRDQSVRSVVMRRSSVCVEKQNSFFRNYE
HFKNILSRRTIKVKTSCPRLSVDVSDNKRENLSQEHLILPNKSREKVNRFKNCLHRVA
EALRAAKENWDQHNPRNSIH"
gene <299693..>300067
/locus_tag="YOL014W"
/db_xref="GeneID:854146"
mRNA <299693..>300067
/locus_tag="YOL014W"
/product="uncharacterized protein"
/transcript_id="NM_001183268.1"
/db_xref="GeneID:854146"
CDS 299693..300067
/locus_tag="YOL014W"
/note="hypothetical protein; mCherry fusion protein
localizes to the cytosol and nucleus"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014628.1"
/db_xref="GeneID:854146"
/db_xref="SGD:S000005374"
/translation="MRPHHFFCGNMGVMYTAMSGYETEDAQAYWACGRAYESAFATLT
KKVPGTTFSADMPTSTWHGVLDCGYSSSINVAENKSSPIDYWNCGRTYARNYALSDAL
SLKPTNMLQYFLLVLFFICIIL"
repeat_region complement(300683..301021)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007152"
gene 301097..301198
/locus_tag="YNCO0011W"
/db_xref="GeneID:854148"
tRNA join(301097..301132,301163..301198)
/locus_tag="YNCO0011W"
/product="tRNA-Pro"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE
analysis; target of K. lactis zymocin"
/db_xref="GeneID:854148"
/db_xref="SGD:S000006687"
gene complement(<301380..>303035)
/gene="HRD1"
/locus_tag="YOL013C"
/gene_synonym="DER3"
/db_xref="GeneID:854149"
mRNA complement(<301380..>303035)
/gene="HRD1"
/locus_tag="YOL013C"
/gene_synonym="DER3"
/product="E3 ubiquitin-protein ligase HRD1"
/transcript_id="NM_001183267.1"
/db_xref="GeneID:854149"
CDS complement(301380..303035)
/gene="HRD1"
/locus_tag="YOL013C"
/gene_synonym="DER3"
/EC_number="2.3.2.27"
/experiment="EXISTENCE:direct assay:GO:0000838 Hrd1p
ubiquitin ligase ERAD-M complex [PMID:16873066]"
/experiment="EXISTENCE:direct assay:GO:0000839 Hrd1p
ubiquitin ligase ERAD-L complex [PMID:16873066]"
/experiment="EXISTENCE:direct assay:GO:0004842
ubiquitin-protein transferase activity
[PMID:11146622|PMID:11139575]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:27831485]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:9437001|PMID:11139575]"
/experiment="EXISTENCE:direct assay:GO:0030970 retrograde
protein transport, ER to cytosol [PMID:27321670]"
/experiment="EXISTENCE:direct assay:GO:0051865 protein
autoubiquitination [PMID:31713515|PMID:27321670]"
/experiment="EXISTENCE:genetic interaction:GO:0004842
ubiquitin-protein transferase activity [PMID:11146622]"
/experiment="EXISTENCE:genetic interaction:GO:0031505
fungal-type cell wall organization [PMID:18971375]"
/experiment="EXISTENCE:mutant phenotype:GO:0004842
ubiquitin-protein transferase activity [PMID:11146622]"
/experiment="EXISTENCE:mutant phenotype:GO:0030968
endoplasmic reticulum unfolded protein response
[PMID:22298424]"
/experiment="EXISTENCE:mutant phenotype:GO:0030970
retrograde protein transport, ER to cytosol
[PMID:24292014]"
/experiment="EXISTENCE:mutant phenotype:GO:0036503 ERAD
pathway [PMID:11146622|PMID:22298424|PMID:17035235]"
/note="Ubiquitin-protein ligase involved in ER-associated
degradation (ERAD) of misfolded proteins; upon
autoubiquitination triggers retrotranslocation of
misfolded proteins to cytosol for degradation; genetically
linked to the unfolded protein response (UPR); regulated
through association with Hrd3p; contains an H2 ring
finger; likely plays a general role in targeting proteins
that persistently associate with and potentially obstruct
the ER-localized translocon"
/codon_start=1
/product="E3 ubiquitin-protein ligase HRD1"
/protein_id="NP_014630.1"
/db_xref="GeneID:854149"
/db_xref="SGD:S000005373"
/translation="MVPENRRKQLAIFVVVTYLLTFYCVYSATKTSVSFLQVTLKLNE
GFNLMVLSIFILLNSTLLWQLLTKLLFGELRLIEHEHIFERLPFTIINTLFMSSLFHE
RYFFTVAFFGLLLLYLKVFHWILKDRLEALLQSINDSTTMKTLIFSRFSFNLVLLAVV
DYQIITRCISSIYTNQKSDIESTSLYLIQVMEFTMLLIDLLNLFLQTCLNFWEFYRSQ
QSLSNENNHIVHGDPTDENTVESDQSQPVLNDDDDDDDDDRQFTGLEGKFMYEKAIDV
FTRFLKTALHLSMLIPFRMPMMLLKDVVWDILALYQSGTSLWKIWRNNKQLDDTLVTV
TVEQLQNSANDDNICIICMDELIHSPNQQTWKNKNKKPKRLPCGHILHLSCLKNWMER
SQTCPICRLPVFDEKGNVVQTTFTSNSDITTQTTVTDSTGIATDQQGFANEVDLLPTR
TTSPDIRIVPTQNIDTLAMRTRSTSTPSPTWYTFPLHKTGDNSVGSSRSAYEFLITNS
DEKENGIPVKLTIENHEVNSLHGDGGEQIAKKIVIPDKFIQHI"
gene complement(<303579..>303983)
/gene="HTZ1"
/locus_tag="YOL012C"
/gene_synonym="HTA3"
/db_xref="GeneID:854150"
mRNA complement(<303579..>303983)
/gene="HTZ1"
/locus_tag="YOL012C"
/gene_synonym="HTA3"
/product="histone H2AZ"
/transcript_id="NM_001183266.1"
/db_xref="GeneID:854150"
CDS complement(303579..303983)
/gene="HTZ1"
/locus_tag="YOL012C"
/gene_synonym="HTA3"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:11090616|PMID:11081628]"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:11081628]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:24390141]"
/experiment="EXISTENCE:genetic interaction:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:11081628]"
/experiment="EXISTENCE:genetic interaction:GO:0030466
silent mating-type cassette heterochromatin formation
[PMID:11090616]"
/experiment="EXISTENCE:mutant phenotype:GO:0006338
chromatin remodeling [PMID:21357739]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:11081628]"
/experiment="EXISTENCE:mutant phenotype:GO:0006368
transcription elongation by RNA polymerase II
[PMID:21357739]"
/experiment="EXISTENCE:mutant phenotype:GO:0070481
nuclear-transcribed mRNA catabolic process, non-stop decay
[PMID:17660569]"
/note="Histone variant H2A.Z; exchanged for histone H2A in
nucleosomes by the SWR1 complex; involved in
transcriptional regulation through prevention of the
spread of silent heterochromatin; Htz1p-containing
nucleosomes facilitate RNA Pol II passage by affecting
correct assembly and modification status of RNA Pol II
elongation complexes and by favoring efficient nucleosome
remodeling"
/codon_start=1
/product="histone H2AZ"
/protein_id="NP_014631.1"
/db_xref="GeneID:854150"
/db_xref="SGD:S000005372"
/translation="MSGKAHGGKGKSGAKDSGSLRSQSSSARAGLQFPVGRIKRYLKR
HATGRTRVGSKAAIYLTAVLEYLTAEVLELAGNAAKDLKVKRITPRHLQLAIRGDDEL
DSLIRATIASGGVLPHINKALLLKVEKKGSKK"
gene <305349..>307409
/gene="PLB3"
/locus_tag="YOL011W"
/db_xref="GeneID:854151"
mRNA <305349..>307409
/gene="PLB3"
/locus_tag="YOL011W"
/product="lysophospholipase"
/transcript_id="NM_001183265.1"
/db_xref="GeneID:854151"
CDS 305349..307409
/gene="PLB3"
/locus_tag="YOL011W"
/EC_number="3.1.1.5"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005576
extracellular region [PMID:10497163]"
/experiment="EXISTENCE:direct assay:GO:0006660
phosphatidylserine catabolic process [PMID:10497163]"
/experiment="EXISTENCE:direct assay:GO:0046488
phosphatidylinositol metabolic process [PMID:10497163]"
/experiment="EXISTENCE:mutant phenotype:GO:0004622
phosphatidylcholine lysophospholipase A1 activity
[PMID:10497163]"
/experiment="EXISTENCE:mutant phenotype:GO:0006660
phosphatidylserine catabolic process [PMID:10497163]"
/experiment="EXISTENCE:mutant phenotype:GO:0046488
phosphatidylinositol metabolic process [PMID:10497163]"
/note="Phospholipase B (lysophospholipase) involved in
lipid metabolism; hydrolyzes phosphatidylinositol and
phosphatidylserine and displays transacylase activity in
vitro; PLB3 has a paralog, PLB1, that arose from the whole
genome duplication"
/codon_start=1
/product="lysophospholipase"
/protein_id="NP_014632.1"
/db_xref="GeneID:854151"
/db_xref="SGD:S000005371"
/translation="MIRPLCSKIIISYIFAISQFLLAANAWSPTDSYVPGTVSCPDDI
NLVREATSISQNESAWLEKRNKVTSVALKDFLTRATANFSDSSEVLSKLFNDGNSENL
PKIAVAVSGGGYRSMLTGAGVLAAMDNRTEGAYEHGLGGLLQSTTYLSGASGGNWLVG
TLALNNWTSVQDILNNMQNDDSIWDLSDSIVTPGGINIFKTAKRWDHISNAVESKQNA
DYNTSLADIWGRALAYNFFPSLNRGGIGLTWSSIRDFPVFQNAEMPFPISVADGRYPG
TKVINLNATVFEFNPFEMGSWDPSLNSFANVKYLGTNVSNGVPLERGKCTAGFDNAGF
IMGTSSTLFNQFLLRINSTHLPSFITRLARHFLKDLSQDFNDIAVYSPNPFKDTKFLD
SDYTTSIVDSDSLFLVDGGEDDENVPVLPLIQKERDVDIIFAVDNSADMRLAWPDGSS
LVHTYERQFVKQGQGMSFPYVPDTNTFVNLGLNKKPTFFGCDANNLTDLQYIPPLVVY
LPNAEYSFNSNQSAFKLSYSESQRRSMIQNGFEIATRNNFTDDPEFMGCVGCAIIRRK
QQALNITLPPECETCFKNYCWNGTLDTTPLPDVEKDVHHSFINVNSFNSSIGQEESLY
AGSSASQSSSSSSSSSSSSEIPSATATLEKKAATNSGSHLSGISVKFSAMIMLTLLMF
TGAV"
gene <307938..>309041
/gene="RCL1"
/locus_tag="YOL010W"
/db_xref="GeneID:854152"
mRNA <307938..>309041
/gene="RCL1"
/locus_tag="YOL010W"
/product="rRNA-processing endoribonuclease"
/transcript_id="NM_001183264.1"
/db_xref="GeneID:854152"
CDS 307938..309041
/gene="RCL1"
/locus_tag="YOL010W"
/experiment="EXISTENCE:direct assay:GO:0004521 RNA
endonuclease activity [PMID:21849504]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:10790377]"
/experiment="EXISTENCE:direct assay:GO:0008047 enzyme
activator activity [PMID:16307926]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/experiment="EXISTENCE:mutant phenotype:GO:0000447
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from
5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10790377]"
/experiment="EXISTENCE:mutant phenotype:GO:0000472
endonucleolytic cleavage to generate mature 5'-end of
SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:10790377]"
/experiment="EXISTENCE:mutant phenotype:GO:0000480
endonucleolytic cleavage in 5'-ETS of tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:10790377]"
/note="Endonuclease that cleaves pre-rRNA at site A2 for
18S rRNA biogenesis; subunit of U3-containing 90S
preribosome processome complex involved in small ribosomal
subunit assembly; stimulates Bms1p GTPase and U3 binding
activity; similar to RNA cyclase-like proteins but no
cyclase activity detected"
/codon_start=1
/product="rRNA-processing endoribonuclease"
/protein_id="NP_014633.1"
/db_xref="GeneID:854152"
/db_xref="SGD:S000005370"
/translation="MSSSAPKYTTFQGSQNFRLRIVLATLSGKPIKIEKIRSGDLNPG
LKDYEVSFLRLIESVTNGSVIEISYTGTTVIYRPGIIVGGASTHICPSSKPVGYFVEP
MLYLAPFSKKKFSILFKGITASHNDAGIEAIKWGLMPVMEKFGVRECALHTLKRGSPP
LGGGEVHLVVDSLIAQPITMHEIDRPIISSITGVAYSTRVSPSLVNRMIDGAKKVLKN
LQCEVNITADVWRGENSGKSPGWGITLVAQSKQKGWSYFAEDIGDAGSIPEELGEKVA
CQLLEEISKSAAVGRNQLPLAIVYMVIGKEDIGRLRINKEQIDERFIILLRDIKKIFN
TEVFLKPVDEADNEDMIATIKGIGFTNTSKKIA"
gene complement(<309324..>310139)
/gene="MDM12"
/locus_tag="YOL009C"
/db_xref="GeneID:854153"
mRNA complement(<309324..>310139)
/gene="MDM12"
/locus_tag="YOL009C"
/product="ERMES complex subunit MDM12"
/transcript_id="NM_001183263.1"
/db_xref="GeneID:854153"
CDS complement(309324..310139)
/gene="MDM12"
/locus_tag="YOL009C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:9024686]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0044233
mitochondria-associated endoplasmic reticulum membrane
contact site [PMID:37366411]"
/experiment="EXISTENCE:direct assay:GO:0120013 lipid
transfer activity [PMID:29279306]"
/experiment="EXISTENCE:genetic interaction:GO:0015914
phospholipid transport [PMID:19556461]"
/experiment="EXISTENCE:mutant phenotype:GO:0000001
mitochondrion inheritance [PMID:9024686]"
/experiment="EXISTENCE:mutant phenotype:GO:0007031
peroxisome organization [PMID:30908556]"
/experiment="EXISTENCE:mutant phenotype:GO:0015917
aminophospholipid transport [PMID:27469264]"
/experiment="EXISTENCE:mutant phenotype:GO:0070096
mitochondrial outer membrane translocase complex assembly
[PMID:17410204]"
/experiment="EXISTENCE:mutant phenotype:GO:1990456
mitochondrion-endoplasmic reticulum membrane tethering
[PMID:19556461]"
/experiment="EXISTENCE:physical interaction:GO:0032865
ERMES complex [PMID:17410204|PMID:13679517]"
/note="Mitochondrial outer membrane protein, ERMES complex
subunit; required for transmission of mitochondria to
daughter cells; required for mitophagy; may influence
import and assembly of outer membrane beta-barrel
proteins; localizes to mitochondria-ER contact sites;
ERMES complex is often co-localized with peroxisomes and
with concentrated areas of pyruvate dehydrogenase"
/codon_start=1
/product="ERMES complex subunit MDM12"
/protein_id="NP_014634.1"
/db_xref="GeneID:854153"
/db_xref="SGD:S000005369"
/translation="MSFDINWSTLESDNRLNDLIRKHLNSYLQNTQLPSYVSNLRVLD
FDLGKVGPAITLKEITDPLDEFYDSIREEADQETEENNDNKEDSEHICPDRTIANHEG
PKDDFEAPVVMPSPNDIQFLLEVEYKGDLLVTIGADLVLNYPVEKFMTLPVKLSISDI
GLHSLCIVACLSKQLFLSFLCDVSDPALDDNQTVLDPKGPILAATKPLERISIVRSMK
IETEIGEQYQGQGSVLRSVGELEQFLFTIFKDFLRKELAWPSWINLDFNDGDE"
rep_origin 309359..309925
/note="ARS1512; Replication origin; identified in multiple
array studies, confirmed by plasmid-based recombinational
ARS assay"
/db_xref="SGD:S000130135"
gene <310312..>310935
/gene="COQ10"
/locus_tag="YOL008W"
/db_xref="GeneID:854154"
mRNA <310312..>310935
/gene="COQ10"
/locus_tag="YOL008W"
/product="ubiquinone-binding protein COQ10"
/transcript_id="NM_001183262.1"
/db_xref="GeneID:854154"
CDS 310312..310935
/gene="COQ10"
/locus_tag="YOL008W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:16230336]"
/experiment="EXISTENCE:direct assay:GO:0048039 ubiquinone
binding [PMID:16230336]"
/experiment="EXISTENCE:direct assay:GO:0140104 molecular
carrier activity [PMID:16230336]"
/experiment="EXISTENCE:mutant phenotype:GO:0006744
ubiquinone biosynthetic process [PMID:23270816]"
/note="Coenzyme Q (ubiquinone) binding protein; functions
in the delivery of Q<sub>6</sub> to its proper location
for electron transport during respiration; START domain
protein with homologs in bacteria and eukaryotes;
respiratory growth defect of the null mutant is
functionally complemented by human COQ10A"
/codon_start=1
/product="ubiquinone-binding protein COQ10"
/protein_id="NP_014635.1"
/db_xref="GeneID:854154"
/db_xref="SGD:S000005368"
/translation="MVLIIRPSQTLILFRKAMLKPIGRYPLKRNFFGLSGTNHTIREQ
RYVLRKAINAPPSTVYAAVSEVAQYKEFIPYCVDSFVDKRNPVDNKPLIAGLRVGFKQ
YDEEFICNVTCKDTDHTYTVVAETISHNLFHLLISKWTIMPHPNRPNAAMVELLLRFK
FKSRIYNSVSLIFAKTVTELVMNAFAKRAYHLVRLAMLKPSSKEGSP"
gene complement(<311342..>312367)
/gene="CSI2"
/locus_tag="YOL007C"
/db_xref="GeneID:854155"
mRNA complement(<311342..>312367)
/gene="CSI2"
/locus_tag="YOL007C"
/product="Csi2p"
/transcript_id="NM_001183261.1"
/db_xref="GeneID:854155"
CDS complement(311342..312367)
/gene="CSI2"
/locus_tag="YOL007C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762|PMID:15282802|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:15282802]"
/note="hypothetical protein; green fluorescent protein
(GFP)- fusion protein localizes to the mother side of the
bud neck and the vacuole; YOL007C is not an essential
gene"
/codon_start=1
/product="Csi2p"
/protein_id="NP_014636.1"
/db_xref="GeneID:854155"
/db_xref="SGD:S000005367"
/translation="MRLPEISIWKVILLLHLFALQEFQLVSAANLPSLSSSTKAADSS
SKGSSSAKTTTSLGKSSVTSKDVSSSHNVTSSTKMPKITTSASTSLYTNSSLWSNNSV
ISTSSITPSSVYIPVTDGNKFLYQAHHPNGTVFIAFAGCLGAILLSLTGAWIALNIKS
WRSARKENKLRNLENQYQHDPFYFQTNINDDESETSSHSDDSDISEKVLKNNSSRMSL
YTLGSTSVLNLLNNKTDANDNFRSSMFISPTEILQSDANNSNTWSQSNESAIYDSLSS
TPREPGATQILGKFTDSTNPFNYTSYNLSPDSEDRSTPKSNVSQGKVKKYRPPSVHLD
QLLDGKE"
gene complement(<313078..>315387)
/gene="TOP1"
/locus_tag="YOL006C"
/gene_synonym="MAK1; MAK17"
/db_xref="GeneID:854156"
mRNA complement(<313078..>315387)
/gene="TOP1"
/locus_tag="YOL006C"
/gene_synonym="MAK1; MAK17"
/product="DNA topoisomerase 1"
/transcript_id="NM_001183260.1"
/db_xref="GeneID:854156"
CDS complement(313078..315387)
/gene="TOP1"
/locus_tag="YOL006C"
/gene_synonym="MAK1; MAK17"
/EC_number="5.6.2.1"
/experiment="EXISTENCE:direct assay:GO:0003917 DNA
topoisomerase type I (single strand cut, ATP-independent)
activity [PMID:6319395|PMID:6088500]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:6088500]"
/experiment="EXISTENCE:direct assay:GO:0006265 DNA
topological change [PMID:6088500]"
/experiment="EXISTENCE:genetic interaction:GO:0009303 rRNA
transcription [PMID:2436053]"
/experiment="EXISTENCE:mutant phenotype:GO:0000019
regulation of mitotic recombination [PMID:2902925]"
/experiment="EXISTENCE:mutant phenotype:GO:0000183 rDNA
heterochromatin formation [PMID:10082585]"
/experiment="EXISTENCE:mutant phenotype:GO:0003917 DNA
topoisomerase type I (single strand cut, ATP-independent)
activity [PMID:6319395|PMID:2989818]"
/experiment="EXISTENCE:mutant phenotype:GO:0005739
mitochondrion [PMID:7677787]"
/experiment="EXISTENCE:mutant phenotype:GO:0006265 DNA
topological change [PMID:2989818]"
/experiment="EXISTENCE:mutant phenotype:GO:0006271 DNA
strand elongation involved in DNA replication
[PMID:2549254]"
/experiment="EXISTENCE:mutant phenotype:GO:0006325
chromatin organization [PMID:9199287]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:1660829]"
/experiment="EXISTENCE:mutant phenotype:GO:0006368
transcription elongation by RNA polymerase II
[PMID:2840207]"
/experiment="EXISTENCE:mutant phenotype:GO:0007076 mitotic
chromosome condensation [PMID:8895658]"
/experiment="EXISTENCE:mutant phenotype:GO:0007097 nuclear
migration [PMID:8895658]"
/experiment="EXISTENCE:physical interaction:GO:0005730
nucleolus [PMID:10967121]"
/note="Topoisomerase I; nuclear and mitochondrial enzyme
that relieves torsional strain in DNA by cleaving and
re-sealing the phosphodiester backbone; relaxes both
positively and negatively supercoiled DNA; functions in
replication, transcription, and recombination; role in
processing ribonucleoside monophosphates in genomic DNA
into irreversible single-strand breaks; enzymatic activity
and interaction with Nsr1p are negatively regulated by
polyphosphorylation"
/codon_start=1
/product="DNA topoisomerase 1"
/protein_id="NP_014637.1"
/db_xref="GeneID:854156"
/db_xref="SGD:S000005366"
/translation="MTIADASKVNHELSSDDDDDVPLSQTLKKRKVASMNSASLQDEA
EPYDSDEAISKISKKKTKKIKTEPVQSSSLPSPPAKKSATSKPKKIKKEDGDVKVKTT
KKEEQENEKKKREEEEEEDKKAKEEEEEYKWWEKENEDDTIKWVTLKHNGVIFPPPYQ
PLPSHIKLYYDGKPVDLPPQAEEVAGFFAALLESDHAKNPVFQKNFFNDFLQVLKESG
GPLNGIEIKEFSRCDFTKMFDYFQLQKEQKKQLTSQEKKQIRLEREKFEEDYKFCELD
GRREQVGNFKVEPPDLFRGRGAHPKTGKLKRRVNPEDIVLNLSKDAPVPPAPEGHKWG
EIRHDNTVQWLAMWRENIFNSFKYVRLAANSSLKGQSDYKKFEKARQLKSYIDAIRRD
YTRNLKSKVMLERQKAVAIYLIDVFALRAGGEKSEDEADTVGCCSLRYEHVTLKPPNT
VIFDFLGKDSIRFYQEVEVDKQVFKNLTIFKRPPKQPGHQLFDRLDPSILNKYLQNYM
PGLTAKVFRTYNASKTMQDQLDLIPNKGSVAEKILKYNAANRTVAILCNHQRTVTKGH
AQTVEKANNRIQELEWQKIRCKRAILQLDKDLLKKEPKYFEEIDDLTKEDEATIHKRI
IDREIEKYQRKFVRENDKRKFEKEELLPESQLKEWLEKVDEKKQEFEKELKTGEVELK
SSWNSVEKIKAQVEKLEQRIQTSSIQLKDKEENSQVSLGTSKINYIDPRLSVVFCKKY
DVPIEKIFTKTLREKFKWAIESVDENWRF"
gene complement(<315813..>316175)
/gene="RPB11"
/locus_tag="YOL005C"
/db_xref="GeneID:854157"
mRNA complement(<315813..>316175)
/gene="RPB11"
/locus_tag="YOL005C"
/product="DNA-directed RNA polymerase II core subunit
RPB11"
/transcript_id="NM_001183259.1"
/db_xref="GeneID:854157"
CDS complement(315813..316175)
/gene="RPB11"
/locus_tag="YOL005C"
/experiment="EXISTENCE:direct assay:GO:0003899
DNA-directed RNA polymerase activity [PMID:8288647]"
/experiment="EXISTENCE:direct assay:GO:0003968
RNA-directed RNA polymerase activity [PMID:18004386]"
/experiment="EXISTENCE:direct assay:GO:0005665 RNA
polymerase II, core complex [PMID:1331084]"
/experiment="EXISTENCE:mutant phenotype:GO:0006366
transcription by RNA polymerase II [PMID:17179178]"
/experiment="EXISTENCE:mutant phenotype:GO:0006369
termination of RNA polymerase II transcription
[PMID:16537912]"
/note="RNA polymerase II subunit B12.5; part of central
core; similar to Rpc19p and bacterial alpha subunit"
/codon_start=1
/product="DNA-directed RNA polymerase II core subunit
RPB11"
/protein_id="NP_014638.1"
/db_xref="GeneID:854157"
/db_xref="SGD:S000005365"
/translation="MNAPDRFELFLLGEGESKLKIDPDTKAPNAVVITFEKEDHTLGN
LIRAELLNDRKVLFAAYKVEHPFFARFKLRIQTTEGYDPKDALKNACNSIINKLGALK
TNFETEWNLQTLAADDAF"
gene <316938..>321548
/gene="SIN3"
/locus_tag="YOL004W"
/gene_synonym="CPE1; GAM2; RPD1; SDI1; SDS16; UME4"
/db_xref="GeneID:854158"
mRNA <316938..>321548
/gene="SIN3"
/locus_tag="YOL004W"
/gene_synonym="CPE1; GAM2; RPD1; SDI1; SDS16; UME4"
/product="transcriptional regulator SIN3"
/transcript_id="NM_001183258.1"
/db_xref="GeneID:854158"
CDS 316938..321548
/gene="SIN3"
/locus_tag="YOL004W"
/gene_synonym="CPE1; GAM2; RPD1; SDI1; SDS16; UME4"
/experiment="EXISTENCE:direct assay:GO:0032221 Rpd3S
complex [PMID:16286008|PMID:16286007]"
/experiment="EXISTENCE:direct assay:GO:0033698 Rpd3L
complex
[PMID:19040720|PMID:16286007|PMID:16286008|PMID:16314178]"
/experiment="EXISTENCE:direct assay:GO:0070210
Rpd3L-Expanded complex [PMID:19040720]"
/experiment="EXISTENCE:direct assay:GO:0070822 Sin3-type
complex [PMID:9234741]"
/experiment="EXISTENCE:genetic interaction:GO:0000086 G2/M
transition of mitotic cell cycle [PMID:17908798]"
/experiment="EXISTENCE:genetic interaction:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:17908798]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:17158929|PMID:11095743|PMID:2690066]"
/experiment="EXISTENCE:mutant phenotype:GO:0003713
transcription coactivator activity [PMID:14737171]"
/experiment="EXISTENCE:mutant phenotype:GO:0003714
transcription corepressor activity [PMID:9150136]"
/experiment="EXISTENCE:mutant phenotype:GO:0006303
double-strand break repair via nonhomologous end joining
[PMID:14711989]"
/experiment="EXISTENCE:mutant phenotype:GO:0016479
negative regulation of transcription by RNA polymerase I
[PMID:19270272]"
/experiment="EXISTENCE:mutant phenotype:GO:0030174
regulation of DNA-templated DNA replication initiation
[PMID:15143171]"
/experiment="EXISTENCE:mutant phenotype:GO:0034605
cellular response to heat [PMID:20398213]"
/experiment="EXISTENCE:mutant phenotype:GO:0044804
nucleophagy [PMID:31553911]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:14737171|PMID:11095743|PMID:20398213]"
/experiment="EXISTENCE:mutant phenotype:GO:0051321 meiotic
cell cycle [PMID:17158929]"
/experiment="EXISTENCE:mutant phenotype:GO:0061186
negative regulation of silent mating-type cassette
heterochromatin formation [PMID:10388812]"
/experiment="EXISTENCE:mutant phenotype:GO:0061188
negative regulation of rDNA heterochromatin formation
[PMID:10082585|PMID:10388812]"
/experiment="EXISTENCE:mutant phenotype:GO:0070550 rDNA
chromatin condensation [PMID:31553911]"
/experiment="EXISTENCE:physical interaction:GO:0003714
transcription corepressor activity [PMID:9150136]"
/note="Component of both the Rpd3S and Rpd3L histone
deacetylase complexes; involved in transcriptional
repression and activation of diverse processes, including
mating-type switching and meiosis; involved in the
maintenance of chromosomal integrity"
/codon_start=1
/product="transcriptional regulator SIN3"
/protein_id="NP_014639.1"
/db_xref="GeneID:854158"
/db_xref="SGD:S000005364"
/translation="MSQVWHNSNSQSNDVATSNDATGSNERNEKEPSLQGNKPGFVQQ
QQRITLPSLSALSTKEEDRRDSNGQQALTSHAAHILGYPPPHSNAMPSIATDSALKQP
HEYHPRPKSSSSSPSINASLMNAGPAPLPTVGAASFSLSRFDNPLPIKAPVHTEEPKS
YNGLQEEEKATQRPQDCKEVPAGVQPADAPDPSSNHADANDDNNNNENSHDEDADYRP
LNVKDALSYLEQVKFQFSSRPDIYNLFLDIMKDFKSQAIDTPGVIERVSTLFRGYPIL
IQGFNTFLPQGYRIECSSNPDDPIRVTTPMGTTTVNNNISPSGRGTTDAQELGSFPES
DGNGVQQPSNVPMVPSSVYQSEQNQDQQQSLPLLATSSGLPSIQQPEMPAHRQIPQSQ
SLVPQEDAKKNVDVEFSQAISYVNKIKTRFADQPDIYKHFLEILQTYQREQKPINEVY
AQVTHLFQNAPDLLEDFKKFLPDSSASANQQVQHAQQHAQQQHEAQMHAQAQAQAQAQ
AQVEQQKQQQQFLYPASGYYGHPSNRGIPQQNLPPIGSFSPPTNGSTVHEAYQDQQHM
QPPHFMPLPSIVQHGPNMVHQGIANENPPLSDLRTSLTEQYAPSSIQHQQQHPQSISP
IANTQYGDIPVRPEIDLDPSIVPVVPEPTEPIENNISLNEEVTFFEKAKRYIGNKHLY
TEFLKILNLYSQDILDLDDLVEKVDFYLGSNKELFTWFKNFVGYQEKTKCIENIVHEK
HRLDLDLCEAFGPSYKRLPKSDTFMPCSGRDDMCWEVLNDEWVGHPVWASEDSGFIAH
RKNQYEETLFKIEEERHEYDFYIESNLRTIQCLETIVNKIENMTENEKANFKLPPGLG
HTSMTIYKKVIRKVYDKERGFEIIDALHEHPAVTAPVVLKRLKQKDEEWRRAQREWNK
VWRELEQKVFFKSLDHLGLTFKQADKKLLTTKQLISEISSIKVDQTNKKIHWLTPKPK
SQLDFDFPDKNIFYDILCLADTFITHTTAYSNPDKERLKDLLKYFISLFFSISFEKIE
ESLYSHKQNVSESSGSDDGSSIASRKRPYQQEMSLLDILHRSRYQKLKRSNDEDGKVP
QLSEPPEEEPNTIEEEELIDEEAKNPWLTGNLVEEANSQGIIQNRSIFNLFANTNIYI
FFRHWTTIYERLLEIKQMNERVTKEINTRSTVTFAKDLDLLSSQLSEMGLDFVGEDAY
KQVLRLSRRLINGDLEHQWFEESLRQAYNNKAFKLYTIDKVTQSLVKHAHTLMTDAKT
AEIMALFVKDRNASTTSAKDQIIYRLQVRSHMSNTENMFRIEFDKRTLHVSIQYIALD
DLTLKEPKADEDKWKYYVTSYALPHPTEGIPHEKLKIPFLERLIEFGQDIDGTEVDEE
FSPEGISVSTLKIKIQPITYQLHIENGSYDVFTRKATNKYPTIANDNTQKGMVSQKKE
LISKFLDCAVGLRNNLDEAQKLSMQKKWENLKDSIAKTSAGNQGIESETEKGKITKQE
QSDNLDSSTASVLPASITTVPQDDNIETTGNTESSDKGAKIQ"
gene complement(<321858..>322994)
/gene="PFA4"
/locus_tag="YOL003C"
/db_xref="GeneID:854159"
mRNA complement(<321858..>322994)
/gene="PFA4"
/locus_tag="YOL003C"
/product="palmitoyltransferase PFA4"
/transcript_id="NM_001183257.1"
/db_xref="GeneID:854159"
CDS complement(321858..322994)
/gene="PFA4"
/locus_tag="YOL003C"
/EC_number="2.3.1.225"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:16647879|PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0016409
palmitoyltransferase activity
[PMID:16818716|PMID:26224664]"
/experiment="EXISTENCE:mutant phenotype:GO:0018345 protein
palmitoylation [PMID:16818716|PMID:16751107]"
/note="Palmitoyltransferase with autoacylation activity;
required for palmitoylation of amino acid permeases
containing a C-terminal Phe-Trp-Cys site; required for
modification of Chs3p; member of the DHHC family of
putative palmitoyltransferases"
/codon_start=1
/product="palmitoyltransferase PFA4"
/protein_id="NP_014640.1"
/db_xref="GeneID:854159"
/db_xref="SGD:S000005363"
/translation="MPVKLRWPWLGIAIPTFLISFIGYGAHYFILSNFLSVPKQITFE
FCLSMIWLSYYLAICTNPGRPLPNYKPPPDIWRNFCKKCQSYKPERSHHCKTCNQCVL
MMDHHCPWTMNCVGFANYPHFLRFLFWIIVTTSVLFCIQAKRIYFIWQQRHLPGYFFK
KSELIFLTISSPLNSFVLLTITILFLRCLFNQILNGRSQIESWDMDRLESLFNSGRLT
QKLIDNTWRIYPESRSFQNKKDAEEHLTKKRPRFDELVNFPYDFDLYTNALLYLGPIH
LWLWPYGVPTGDGNNFPKNGISKYEANSSLEDHILSLPWPPDGGKTNTVFNHGSSTIE
MRNESGEQLIRTRLPQNGRHASREKWYNDWGESLDDFGVDVDME"
gene complement(<323411..>324364)
/gene="IZH2"
/locus_tag="YOL002C"
/gene_synonym="PHO36"
/db_xref="GeneID:854160"
mRNA complement(<323411..>324364)
/gene="IZH2"
/locus_tag="YOL002C"
/gene_synonym="PHO36"
/product="PAQR-type receptor"
/transcript_id="NM_001183256.1"
/db_xref="GeneID:854160"
CDS complement(323411..324364)
/gene="IZH2"
/locus_tag="YOL002C"
/gene_synonym="PHO36"
/experiment="EXISTENCE:direct assay:GO:0000122 negative
regulation of transcription by RNA polymerase II
[PMID:18625204]"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:15664187]"
/experiment="EXISTENCE:mutant phenotype:GO:0006882
intracellular zinc ion homeostasis [PMID:15060275]"
/experiment="EXISTENCE:mutant phenotype:GO:0009636
response to toxic substance [PMID:15664187]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:15664187]"
/note="Plasma membrane receptor for plant antifungal
osmotin; involved in zinc ion homeostasis, apoptosis;
negatively regulates ZRT1 and other functionally divergent
genes through CCCTC promoter motif (IzRE); modulates FET3
activity in iron-independent manner; affects gene
expression by influencing balance of competition between
Msn2p/Msn4p and Nrg1p/Nrg2p for binding to IzRE;
transcription regulated by Zap1p, zinc, fatty acid levels;
homolog of mammalian adiponectin receptor"
/codon_start=1
/product="PAQR-type receptor"
/protein_id="NP_014641.2"
/db_xref="GeneID:854160"
/db_xref="SGD:S000005362"
/translation="MSTLLERTKSVQELKKRAAGKTSANPAEVAKAKKVLRRLYSWDE
IPEWQRDNDFILHGYVKETSSFIETFKSLFYLHNESVNIYSHLIPALGFFTVLLLDKS
TIKVFATTTWLDHMVIDLFYSGAFACLILSSSFHCLKSHSLRIATLGNKLDYLGICIL
IVTSMVSILYYGYFEKFSLFCLFALITVSFGIACSIVSLKDKFRKREWRPYRAGLFVC
FGLSSIIPIFSGLYCYSFSEIWTQIQLFWVLLGGVLYIIGAVLYGMRFPEKICPGKFD
IWGHSHQLFHFLVVIAALCHLRGLLNSYELVHIKMENGIVS"
gene <325249..>326130
/gene="PHO80"
/locus_tag="YOL001W"
/gene_synonym="AGS3; TUP7; VAC5"
/db_xref="GeneID:854161"
mRNA <325249..>326130
/gene="PHO80"
/locus_tag="YOL001W"
/gene_synonym="AGS3; TUP7; VAC5"
/product="Pho80p"
/transcript_id="NM_001183255.1"
/db_xref="GeneID:854161"
CDS 325249..326130
/gene="PHO80"
/locus_tag="YOL001W"
/gene_synonym="AGS3; TUP7; VAC5"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9853758]"
/experiment="EXISTENCE:direct assay:GO:0016538
cyclin-dependent protein serine/threonine kinase regulator
activity [PMID:8108735]"
/experiment="EXISTENCE:direct assay:GO:1990860 Pho85-Pho80
CDK-cyclin complex [PMID:8108735]"
/experiment="EXISTENCE:genetic interaction:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:3915785]"
/experiment="EXISTENCE:genetic interaction:GO:0045936
negative regulation of phosphate metabolic process
[PMID:3915785]"
/experiment="EXISTENCE:genetic interaction:GO:0050849
negative regulation of calcium-mediated signaling
[PMID:16455487]"
/experiment="EXISTENCE:mutant phenotype:GO:0016242
negative regulation of macroautophagy [PMID:20417603]"
/experiment="EXISTENCE:mutant phenotype:GO:0030003
intracellular monoatomic cation homeostasis
[PMID:20429018]"
/experiment="EXISTENCE:mutant phenotype:GO:0032880
regulation of protein localization [PMID:16455487]"
/experiment="EXISTENCE:mutant phenotype:GO:0042144 vacuole
fusion, non-autophagic [PMID:27252384]"
/experiment="EXISTENCE:physical interaction:GO:0000307
cyclin-dependent protein kinase holoenzyme complex
[PMID:8108735]"
/note="Cyclin; interacts with cyclin-dependent kinase
Pho85p; regulates the response to nutrient levels and
environmental conditions, including the response to
phosphate limitation and stress-dependent calcium
signaling"
/codon_start=1
/product="Pho80p"
/protein_id="NP_014642.1"
/db_xref="GeneID:854161"
/db_xref="SGD:S000005361"
/translation="MESTSGERSENIHEDQGIPKVILPADFNKCSRTDLVVLISRMLV
SLIAINENSATKKSDDQITLTRYHSKIPPNISIFNYFIRLTKFSSLEHCVLMTSLYYI
DLLQTVYPDFTLNSLTAHRFLLTATTVATKGLCDSFSTNAHYAKVGGVRCHELNILEN
DFLKRVNYRIIPRDHNITLCSIEQKQKKFVIDKNALGSLDLDSYSYVNRPKSGYNVLD
KYYRRIVQLVGSFNASPDKSRKVDYVLPPNIDIVSESGSQTTQLKGSSSPNSHSSQKR
YSEAKDAHIYNKRSKPD"
centromere complement(326584..326702)
/note="CEN15; Chromosome XV centromere"
/db_xref="SGD:S000006476"
centromere complement(326584..326608)
/note="CEN15_CDEIII of CEN15"
centromere complement(326609..326692)
/note="CEN15_CDEII of CEN15"
centromere complement(326693..326702)
/note="CEN15_CDEI of CEN15"
gene <326832..>329033
/gene="RRP6"
/locus_tag="YOR001W"
/db_xref="GeneID:854162"
mRNA <326832..>329033
/gene="RRP6"
/locus_tag="YOR001W"
/product="exosome nuclease subunit RRP6"
/transcript_id="NM_001183420.1"
/db_xref="GeneID:854162"
CDS 326832..329033
/gene="RRP6"
/locus_tag="YOR001W"
/experiment="EXISTENCE:direct assay:GO:0000175 3'-5'-RNA
exonuclease activity [PMID:10611239]"
/experiment="EXISTENCE:direct assay:GO:0000176 nuclear
exosome (RNase complex) [PMID:19046973|PMID:10465791]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10611239|PMID:23580640]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:16541108|PMID:31662437]"
/experiment="EXISTENCE:direct assay:GO:0042134 rRNA
primary transcript binding [PMID:31662437]"
/experiment="EXISTENCE:direct assay:GO:0071038
TRAMP-dependent tRNA surveillance pathway [PMID:15828860]"
/experiment="EXISTENCE:genetic interaction:GO:0034473 U1
snRNA 3'-end processing [PMID:10508172]"
/experiment="EXISTENCE:genetic interaction:GO:0034475 U4
snRNA 3'-end processing [PMID:10716935|PMID:10508172]"
/experiment="EXISTENCE:genetic interaction:GO:0034476 U5
snRNA 3'-end processing [PMID:10508172]"
/experiment="EXISTENCE:genetic interaction:GO:0071028
nuclear mRNA surveillance
[PMID:11586364|PMID:17410208|PMID:12417728]"
/experiment="EXISTENCE:genetic interaction:GO:0071035
nuclear polyadenylation-dependent rRNA catabolic process
[PMID:16541108]"
/experiment="EXISTENCE:genetic interaction:GO:0071038
TRAMP-dependent tRNA surveillance pathway [PMID:18456844]"
/experiment="EXISTENCE:genetic interaction:GO:0071039
nuclear polyadenylation-dependent CUT catabolic process
[PMID:15935759]"
/experiment="EXISTENCE:mutant phenotype:GO:0000467
exonucleolytic trimming to generate mature 3'-end of 5.8S
rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S
rRNA, LSU-rRNA)
[PMID:9582370|PMID:10508172|PMID:10465791|PMID:18940861]"
/experiment="EXISTENCE:mutant phenotype:GO:0000973
post-transcriptional tethering of RNA polymerase II gene
DNA at nuclear periphery [PMID:18614049]"
/experiment="EXISTENCE:mutant phenotype:GO:0032204
regulation of telomere maintenance [PMID:28160602]"
/experiment="EXISTENCE:mutant phenotype:GO:0034473 U1
snRNA 3'-end processing [PMID:10508172]"
/experiment="EXISTENCE:mutant phenotype:GO:0034475 U4
snRNA 3'-end processing
[PMID:10611222|PMID:10508172|PMID:16373491]"
/experiment="EXISTENCE:mutant phenotype:GO:0034476 U5
snRNA 3'-end processing [PMID:10508172]"
/experiment="EXISTENCE:mutant phenotype:GO:0071028 nuclear
mRNA surveillance [PMID:27124216]"
/experiment="EXISTENCE:mutant phenotype:GO:0071035 nuclear
polyadenylation-dependent rRNA catabolic process
[PMID:10465791|PMID:15572680|PMID:16431988|PMID:18007593|P
MID:18940861|PMID:15173578]"
/experiment="EXISTENCE:mutant phenotype:GO:0071036 nuclear
polyadenylation-dependent snoRNA catabolic process
[PMID:16373491]"
/experiment="EXISTENCE:mutant phenotype:GO:0071037 nuclear
polyadenylation-dependent snRNA catabolic process
[PMID:16431988]"
/experiment="EXISTENCE:mutant phenotype:GO:0071039 nuclear
polyadenylation-dependent CUT catabolic process
[PMID:18007593|PMID:16973436|PMID:15935759]"
/experiment="EXISTENCE:mutant phenotype:GO:0071040 nuclear
polyadenylation-dependent antisense transcript catabolic
process [PMID:18022365]"
/experiment="EXISTENCE:mutant phenotype:GO:0071042 nuclear
polyadenylation-dependent mRNA catabolic process
[PMID:19369424|PMID:16373491]"
/experiment="EXISTENCE:mutant phenotype:GO:0071044 histone
mRNA catabolic process [PMID:17179095]"
/experiment="EXISTENCE:mutant phenotype:GO:0071051
poly(A)-dependent snoRNA 3'-end processing
[PMID:10611222|PMID:16373491|PMID:18951092]"
/note="Nuclear exosome exonuclease component; has 3'-5'
exonuclease activity that is regulated by Lrp1p; involved
in RNA processing, maturation, surveillance, degradation,
tethering, and export; role in sn/snoRNAs precursor
degradation; forms a stable heterodimer with Lrp1p; has
similarity to E. coli RNase D and to human PM-Sc1 100
(EXOSC10); mutant displays reduced transcription
elongation in the G-less-based"
/codon_start=1
/product="exosome nuclease subunit RRP6"
/protein_id="NP_014643.1"
/db_xref="GeneID:854162"
/db_xref="SGD:S000005527"
/translation="MTSENPDVLLSRVINVVRAASSLASQDVDFYKNLDRGFSKDLKS
KADKLADMANEIILSIDEHHESFELKEEDISDLWNNFGNIMDNLLEMSDHSLDKLNCA
INSKSRGSDLQYLGEFSGKNFSPTKRVEKPQLKFKSPIDNSESHPFIPLLKEKPNALK
PLSESLRLVDDDENNPSHYPHPYEYEIDHQEYSPEILQIREEIPSKSWDDSVPIWVDT
STELESMLEDLKNTKEIAVDLEHHDYRSYYGIVCLMQISTRERDYLVDTLKLRENLHI
LNEVFTNPSIVKVFHGAFMDIIWLQRDLGLYVVGLFDTYHASKAIGLPRHSLAYLLEN
FANFKTSKKYQLADWRIRPLSKPMTAYARADTHFLLNIYDQLRNKLIESNKLAGVLYE
SRNVAKRRFEYSKYRPLTPSSEVYSPIEKESPWKILMYQYNIPPEREVLVRELYQWRD
LIARRDDESPRFVMPNQLLAALVAYTPTDVIGVVSLTNGVTEHVRQNAKLLANLIRDA
LRNIKNTNEEATPIPSSETKADGILLETISVPQIRDVMERFSVLCNSNISKSRAKPVT
NSSILLGKILPREEHDIAYSKDGLPNKVKTEDIRIRAQNFKSALANLEDIIFEIEKPL
VVPVKLEEIKTVDPASAPNHSPEIDNLDDLVVLKKKNIQKKQPAKEKGVTEKDAVDYS
KIPNILSNKPGQNNRQQKKRRFDPSSSDSNGPRAAKKRRPAAKGKNLSFKR"
gene <329417..>331051
/gene="ALG6"
/locus_tag="YOR002W"
/db_xref="GeneID:854163"
mRNA <329417..>331051
/gene="ALG6"
/locus_tag="YOR002W"
/product="dolichyl-P-Glc:Man(9)GlcNAc(2)-PP-dolichol
alpha-1,3-glucosyltransferase"
/transcript_id="NM_001183421.1"
/db_xref="GeneID:854163"
CDS 329417..331051
/gene="ALG6"
/locus_tag="YOR002W"
/EC_number="2.4.1.267"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0005783
endoplasmic reticulum [PMID:8877369]"
/experiment="EXISTENCE:mutant phenotype:GO:0006488
dolichol-linked oligosaccharide biosynthetic process
[PMID:10336995]"
/experiment="EXISTENCE:mutant phenotype:GO:0009060 aerobic
respiration [PMID:15794922]"
/experiment="EXISTENCE:mutant phenotype:GO:0009101
glycoprotein biosynthetic process [PMID:8877369]"
/experiment="EXISTENCE:mutant phenotype:GO:0016758
hexosyltransferase activity [PMID:8877369]"
/experiment="EXISTENCE:mutant phenotype:GO:0042281
dolichyl pyrophosphate Man9GlcNAc2
alpha-1,3-glucosyltransferase activity [PMID:10336995]"
/note="ER-luminal alpha 1,3 glucosyltransferase; involved
in transfer of oligosaccharides from dolichyl
pyrophosphate to asparagine residues of proteins during
N-linked protein glycosylation; C998T transition in human
ortholog ALG6 causes carbohydrate-deficient glycoprotein
syndrome type-Ic; wild-type human ortholog ALG6 can
partially complement yeast alg6 mutant"
/codon_start=1
/product="dolichyl-P-Glc:Man(9)GlcNAc(2)-PP-dolichol
alpha-1,3-glucosyltransferase"
/protein_id="NP_014644.1"
/db_xref="GeneID:854163"
/db_xref="SGD:S000005528"
/translation="MAIGKRLLVNKPAEESFYASPMYDFLYPFRPVGNQWLPEYIIFV
CAVILRCTIGLGPYSGKGSPPLYGDFEAQRHWMEITQHLPLSKWYWYDLQYWGLDYPP
LTAFHSYLLGLIGSFFNPSWFALEKSRGFESPDNGLKTYMRSTVIISDILFYFPAVIY
FTKWLGRYRNQSPIGQSIAASAILFQPSLMLIDHGHFQYNSVMLGLTAYAINNLLDEY
YAMAAVCFVLSICFKQMALYYAPIFFAYLLSRSLLFPKFNIARLTVIAFATLATFAII
FAPLYFLGGGLKNIHQCIHRIFPFARGIFEDKVANFWCVTNVFVKYKERFTIQQLQLY
SLIATVIGFLPAMIMTLLHPKKHLLPYVLIACSMSFFLFSFQVHEKTILIPLLPITLL
YSSTDWNVLSLVSWINNVALFTLWPLLKKDGLHLQYAVSFLLSNWLIGNFSFITPRFL
PKSLTPGPSISSINSDYRRRSLLPYNVVWKSFIIGTYIAMGFYHFLDQFVAPPSKYPD
LWVLLNCAVGFICFSIFWLWSYYKIFTSGSKSMKDL"
gene <331455..>332891
/gene="YSP3"
/locus_tag="YOR003W"
/db_xref="GeneID:854164"
mRNA <331455..>332891
/gene="YSP3"
/locus_tag="YOR003W"
/product="putative subtilisin-like protease YSP3"
/transcript_id="NM_001183422.1"
/db_xref="GeneID:854164"
CDS 331455..332891
/gene="YSP3"
/locus_tag="YOR003W"
/experiment="EXISTENCE:direct assay:GO:0000328 fungal-type
vacuole lumen [PMID:17651441]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/note="Putative precursor of the subtilisin-like protease
III; SWAT-GFP and mCherry fusion proteins localize to the
endoplasmic reticulum; YSP3 has a paralog, PRB1, that
arose from the whole genome duplication"
/codon_start=1
/product="putative subtilisin-like protease YSP3"
/protein_id="NP_014645.1"
/db_xref="GeneID:854164"
/db_xref="SGD:S000005529"
/translation="MKFSTILPILWANCCLCMIIPDFDGIVRFIENIDGTRSVRAGEG
LGQHDPGNFHTEHQHVAHKTEFLPYRYVIVFNEDISLQQIQSHMQVVQKDHSTSVGKL
TENDAFWRVISSSVSSKSQFGGIDNFFDINGLFRGYTGYFTDEIIKIISQDPIIKFVE
QETTVKISNSSLQEEAPWGLHRVSHREKPKYGQDLEYLYEDAAGKGVTSYVLDTGIDT
EHEDFEGRAEWGAVIPANDEASDLNGHGTHCAGIIGSKHFGVAKNTKIVAVKVLRSNG
EGTVSDVIKGIEYVTKEHIESSKKKNKEFKGSTANLSLGSSKSLAMEMAVNAAVDSGV
HFAIAAGNEDEDACLSSPAGAEKSITVGASTFSDDRAFFSNWGTCVDVFAPGINIMST
YIGSRNATLSLSGTSMASPHVAGILSYFLSLQPAPDSEFFNDAPSPQELKEKVLKFST
QGVLGDIGDDTPNKLIYNGGGKKLDGFW"
gene <333592..>334356
/gene="UTP23"
/locus_tag="YOR004W"
/db_xref="GeneID:854165"
mRNA <333592..>334356
/gene="UTP23"
/locus_tag="YOR004W"
/product="rRNA-binding ribosome biosynthesis protein
UTP23"
/transcript_id="NM_001183423.1"
/db_xref="GeneID:854165"
CDS 333592..334356
/gene="UTP23"
/locus_tag="YOR004W"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:16769905|PMID:14690591]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0032040
small-subunit processome [PMID:16769905]"
/experiment="EXISTENCE:direct assay:GO:0070181 small
ribosomal subunit rRNA binding [PMID:24152547]"
/experiment="EXISTENCE:mutant phenotype:GO:0000447
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from
5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16769905]"
/experiment="EXISTENCE:mutant phenotype:GO:0000472
endonucleolytic cleavage to generate mature 5'-end of
SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:16769905]"
/experiment="EXISTENCE:mutant phenotype:GO:0000480
endonucleolytic cleavage in 5'-ETS of tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:16769905]"
/experiment="EXISTENCE:mutant phenotype:GO:0070181 small
ribosomal subunit rRNA binding [PMID:24152547]"
/experiment="EXISTENCE:physical interaction:GO:0032040
small-subunit processome [PMID:16769905]"
/note="Component of the small subunit processome; involved
in 40S ribosomal subunit biogenesis; interacts with snR30
and is required for dissociation of snR30 from large
pre-ribosomal particles; has homology to PINc domain
protein Fcf1p, although the PINc domain of Utp23p is not
required for function; essential protein"
/codon_start=1
/product="rRNA-binding ribosome biosynthesis protein
UTP23"
/protein_id="NP_014646.1"
/db_xref="GeneID:854165"
/db_xref="SGD:S000005530"
/translation="MRQKRAKSYRKQLLVYSHTFKFREPYQVLVDNQLVLECNNSNFN
LPSGLKRTLQADVKVMITQCCIQALYETRNDGAINLAKQFERRRCNHSFKDPKSPAEC
IESVVNISGANKHRYVVASQDIDLRRKLRTVPGVPLIHLTRSVMVMEPLSTASAKASK
ITEEQKLYKGLNDPNIEKLQESGDGSGKESITKKRKLGPKAPNPLSVKKKKKVNSPSD
EVKDKEDTSKEKKKRRRRKHKSNTNVPVSNGTTAAQ"
gene complement(<334509..>337343)
/gene="DNL4"
/locus_tag="YOR005C"
/gene_synonym="LIG4"
/db_xref="GeneID:854166"
mRNA complement(<334509..>337343)
/gene="DNL4"
/locus_tag="YOR005C"
/gene_synonym="LIG4"
/product="DNA ligase (ATP) DNL4"
/transcript_id="NM_001183424.1"
/db_xref="GeneID:854166"
CDS complement(334509..337343)
/gene="DNL4"
/locus_tag="YOR005C"
/gene_synonym="LIG4"
/EC_number="6.5.1.1"
/experiment="EXISTENCE:direct assay:GO:0003910 DNA ligase
(ATP) activity [PMID:9271115|PMID:9242411]"
/experiment="EXISTENCE:direct assay:GO:0006303
double-strand break repair via nonhomologous end joining
[PMID:15342630]"
/experiment="EXISTENCE:mutant phenotype:GO:0003910 DNA
ligase (ATP) activity [PMID:9271115]"
/experiment="EXISTENCE:mutant phenotype:GO:0006303
double-strand break repair via nonhomologous end joining
[PMID:9242411]"
/experiment="EXISTENCE:mutant phenotype:GO:0043007
maintenance of rDNA [PMID:20541983]"
/experiment="EXISTENCE:physical interaction:GO:0032807 DNA
ligase IV complex [PMID:17567543]"
/note="DNA ligase required for nonhomologous end-joining
(NHEJ); forms stable heterodimer with required cofactor
Lif1p, interacts with Nej1p; involved in meiosis, not
essential for vegetative growth; mutations in human
ortholog lead to ligase IV syndrome and Dubowitz syndrome"
/codon_start=1
/product="DNA ligase (ATP) DNL4"
/protein_id="NP_014647.1"
/db_xref="GeneID:854166"
/db_xref="SGD:S000005531"
/translation="MISALDSIPEPQNFAPSPDFKWLCEELFVKIHEVQINGTAGTGK
SRSFKYYEIISNFVEMWRKTVGNNIYPALVLALPYRDRRIYNIKDYVLIRTICSYLKL
PKNSATEQRLKDWKQRVGKGGNLSSLLVEEIAKRRAEPSSKAITIDNVNHYLDSLSGD
RFASGRGFKSLVKSKPFLHCVENMSFVELKYFFDIVLKNRVIGGQEHKLLNCWHPDAQ
DYLSVISDLKVVTSKLYDPKVRLKDDDLSIKVGFAFAPQLAKKVNLSYEKICRTLHDD
FLVEEKMDGERIQVHYMNYGESIKFFSRRGIDYTYLYGASLSSGTISQHLRFTDSVKE
CVLDGEMVTFDAKRRVILPFGLVKGSAKEALSFNSINNVDFHPLYMVFDLLYLNGTSL
TPLPLHQRKQYLNSILSPLKNIVEIVRSSRCYGVESIKKSLEVAISLGSEGVVLKYYN
SSYNVASRNNNWIKVKPEYLEEFGENLDLIVIGRDSGKKDSFMLGLLVLDEEEYKKHQ
GDSSEIVDHSSQEKHIQNSRRRVKKILSFCSIANGISQEEFKEIDRKTRGHWKRTSEV
APPASILEFGSKIPAEWIDPSESIVLEIKSRSLDNTETNMQKYATNCTLYGGYCKRIR
YDKEWTDCYTLNDLYESRTVKSNPSYQAERSQLGLIRKKRKRVLISDSFHQNRKQLPI
SNIFAGLLFYVLSDYVTEDTGIRITRAELEKTIVEHGGKLIYNVILKRHSIGDVRLIS
CKTTTECKALIDRGYDILHPNWVLDCIAYKRLILIEPNYCFNVSQKMRAVAEKRVDCL
GDSFENDISETKLSSLYKSQLSLPPMGELEIDSEVRRFPLFLFSNRIAYVPRRKISTE
DDIIEMKIKLFGGKITDQQSLCNLIIIPYTDPILRKDCMNEVHEKIKEQIKASDTIPK
IARVVAPEWVDHSINENCQVPEEDFPVVNY"
rep_origin 337424..337491
/note="ARS1513; Autonomously Replicating Sequence"
/db_xref="SGD:S000118489"
gene complement(<337680..>338621)
/gene="TSR3"
/locus_tag="YOR006C"
/db_xref="GeneID:854167"
mRNA complement(<337680..>338621)
/gene="TSR3"
/locus_tag="YOR006C"
/product="ribosome biogenesis protein TSR3"
/transcript_id="NM_001183425.1"
/db_xref="GeneID:854167"
CDS complement(337680..338621)
/gene="TSR3"
/locus_tag="YOR006C"
/EC_number="2.5.1.157"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276|PMID:14562095|PMID:22842922|PMID:27084949]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:36164978]"
/experiment="EXISTENCE:mutant phenotype:GO:0030490
maturation of SSU-rRNA [PMID:19806183]"
/experiment="EXISTENCE:mutant phenotype:GO:0106388 rRNA
small subunit aminocarboxypropyltransferase activity
[PMID:27084949]"
/note="Protein required for 20S pre-rRNA processing;
involved in processing of the 20S pre-rRNA at site D to
generate mature 18S rRNA; green fluorescent protein
(GFP)-fusion protein localizes to both the cytoplasm and
the nucleus; relative distribution to the nucleus
increases upon DNA replication stress; also detected in
peroxisomes"
/codon_start=1
/product="ribosome biogenesis protein TSR3"
/protein_id="NP_014648.1"
/db_xref="GeneID:854167"
/db_xref="SGD:S000005532"
/translation="MGKGKNKMHEPKNGRPQRGANGHSSRQNHRRMEMKYDNSEKMKF
PVKLAMWDFDHCDPKRCSGKKLERLGLIKSLRVGQKFQGIVVSPNGKGVVCPDDLEIV
EQHGASVVECSWARLEEVPFNKIGGKHERLLPYLVAANQVNYGRPWRLNCVEALAACF
AIVGRMDWASELLSHFSWGMGFLELNKELLEIYQQCTDCDSVKRAEEEWLQKLEKETQ
ERKSRAKEEDIWMMGNINRRGNGSQSDTSESEENSEQSDLEGNNQCIEYDSLGNAIRI
DNMKSREAQSEESEDEESGSKENGEPLSYDPLGNLIR"
gene complement(<338938..>339978)
/gene="SGT2"
/locus_tag="YOR007C"
/db_xref="GeneID:854168"
mRNA complement(<338938..>339978)
/gene="SGT2"
/locus_tag="YOR007C"
/product="Sgt2p"
/transcript_id="NM_001183426.1"
/db_xref="GeneID:854168"
CDS complement(338938..339978)
/gene="SGT2"
/locus_tag="YOR007C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0006620
post-translational protein targeting to endoplasmic
reticulum membrane [PMID:20850366]"
/experiment="EXISTENCE:direct assay:GO:0072380 TRC complex
[PMID:20850366]"
/experiment="EXISTENCE:mutant phenotype:GO:0009408
response to heat [PMID:12482202]"
/experiment="EXISTENCE:mutant phenotype:GO:0060090
molecular adaptor activity [PMID:20850366]"
/experiment="EXISTENCE:physical interaction:GO:0030544
Hsp70 protein binding [PMID:21832041]"
/experiment="EXISTENCE:physical interaction:GO:0031072
heat shock protein binding [PMID:21832041|PMID:17441508]"
/experiment="EXISTENCE:physical interaction:GO:0051879
Hsp90 protein binding [PMID:21832041|PMID:17441508]"
/note="Glutamine-rich cytoplasmic entry cochaperone;
serves as a scaffold bringing together Get4, Get5p, and
other TRC complex members that are required to mediate
posttranslational insertion of tail-anchored proteins into
the ER membrane; interacts with the prion domain of
Sup35p; amyloid sensor; plays a role in targeting
chaperones to prion aggregates; similar to human
cochaperone SGT; forms cytoplasmic foci upon DNA
replication stress"
/codon_start=1
/product="Sgt2p"
/protein_id="NP_014649.1"
/db_xref="GeneID:854168"
/db_xref="SGD:S000005533"
/translation="MSASKEEIAALIVNYFSSIVEKKEISEDGADSLNVAMDCISEAF
GFEREAVSGILGKSEFKGQHLADILNSASRVPESNKKDDAENVEINIPEDDAETKAKA
EDLKMQGNKAMANKDYELAINKYTEAIKVLPTNAIYYANRAAAHSSLKEYDQAVKDAE
SAISIDPSYFRGYSRLGFAKYAQGKPEEALEAYKKVLDIEGDNATEAMKRDYESAKKK
VEQSLNLEKTVPEQSRDADVDASQGASAGGLPDLGSLLGGGLGGLMNNPQLMQAAQKM
MSNPGAMQNIQKMMQDPSIRQMAEGFASGGGTPNLSDLMNNPALRNMAGNLFGGAGAQ
STDETPDNENKQ"
gene complement(340299..340371)
/locus_tag="YNCO0012C"
/db_xref="GeneID:854169"
tRNA complement(340299..340371)
/locus_tag="YNCO0012C"
/product="tRNA-Arg"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE
analysis; one of 6 nuclear tRNA genes containing the
tDNA-anticodon ACG (converted to ICG in the mature tRNA),
decodes CGU, CGC, and probably CGA codons into arginine,
one of 19 nuclear tRNAs for arginine"
/db_xref="GeneID:854169"
/db_xref="SGD:S000006705"
repeat_region complement(340598..340898)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007153"
gene complement(<341278..>342414)
/gene="SLG1"
/locus_tag="YOR008C"
/gene_synonym="HCS77; WSC1"
/db_xref="GeneID:854170"
mRNA complement(<341278..>342414)
/gene="SLG1"
/locus_tag="YOR008C"
/gene_synonym="HCS77; WSC1"
/product="Slg1p"
/transcript_id="NM_001183427.1"
/db_xref="GeneID:854170"
CDS complement(341278..342414)
/gene="SLG1"
/locus_tag="YOR008C"
/gene_synonym="HCS77; WSC1"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:20652590|PMID:9391108]"
/experiment="EXISTENCE:direct assay:GO:0005933 cellular
bud [PMID:20652590]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:17761172|PMID:20652590]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807]"
/experiment="EXISTENCE:genetic interaction:GO:0004888
transmembrane signaling receptor activity [PMID:10430578]"
/experiment="EXISTENCE:genetic interaction:GO:0006970
response to osmotic stress [PMID:14871941]"
/experiment="EXISTENCE:mutant phenotype:GO:0000425
pexophagy [PMID:20385774]"
/experiment="EXISTENCE:mutant phenotype:GO:0006897
endocytosis [PMID:30361468]"
/experiment="EXISTENCE:mutant phenotype:GO:0007266 Rho
protein signal transduction [PMID:10508863|PMID:10348843]"
/experiment="EXISTENCE:mutant phenotype:GO:0009408
response to heat [PMID:10348843|PMID:10508863]"
/experiment="EXISTENCE:mutant phenotype:GO:0030010
establishment of cell polarity
[PMID:10508863|PMID:24326069]"
/experiment="EXISTENCE:mutant phenotype:GO:0030036 actin
cytoskeleton organization [PMID:10508863]"
/experiment="EXISTENCE:mutant phenotype:GO:0031505
fungal-type cell wall organization [PMID:10348843]"
/experiment="EXISTENCE:mutant phenotype:GO:0045807
positive regulation of endocytosis
[PMID:24326069|PMID:14593073]"
/note="Sensor-transducer of the stress-activated PKC1-MPK1
kinase pathway; involved in maintenance of cell wall
integrity; required for mitophagy; involved in
organization of the actin cytoskeleton; secretory pathway
Wsc1p is required for the arrest of secretion response"
/codon_start=1
/product="Slg1p"
/protein_id="NP_014650.1"
/db_xref="GeneID:854170"
/db_xref="SGD:S000005534"
/translation="MRPNKTSLLLALLSILSQANAYEYVNCFSSLPSDFSKADSYNWQ
SSSHCNSECSAKGASYFALYNHSECYCGDTNPSGSESTSSSCNTYCFGYSSEMCGGED
AYSVYQLDSDTNSNSISSSDSSTESTSASSSTTSSTTSSTTSTTSSTTSSTTSSMASS
STVQNSPESTQAAASISTSQSSSTVTSESSLTSDTLATSSTSSQSQDATSIIYSTTFH
TEGGSTIFVTNTITASAQNSGSATGTAGSDSTSGSKTHKKKANVGAIVGGVVGGVVGA
VAIALCILLIVRHINMKREQDRMEKEYQEAIKPVEYPDKLYASSFSSNHGPSSGSFEE
EHTKGQTDINPFDDSRRISNGTFINGGPGGKNNVLTVVNPDEAD"
gene complement(<342857..>343081)
/locus_tag="YOR008C-A"
/db_xref="GeneID:854171"
mRNA complement(<342857..>343081)
/locus_tag="YOR008C-A"
/product="uncharacterized protein"
/transcript_id="NM_001184305.1"
/db_xref="GeneID:854171"
CDS complement(342857..343081)
/locus_tag="YOR008C-A"
/note="hypothetical protein; includes a potential
transmembrane domain; deletion results in slightly
lengthened telomeres; SWAT-GFP and mCherry fusion proteins
localize to the endoplasmic reticulum and vacuole
respectively"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014651.1"
/db_xref="GeneID:854171"
/db_xref="SGD:S000006431"
/translation="MWRSYLVFLFFMTPRIQTYCPVPVLRSMAVLNIISPLIIFVSPI
KKQDSLHSSACYANLTLVEKLQLWHSMSND"
gene <344335..>345798
/gene="TIR4"
/locus_tag="YOR009W"
/db_xref="GeneID:854173"
mRNA <344335..>345798
/gene="TIR4"
/locus_tag="YOR009W"
/product="Tir4p"
/transcript_id="NM_001183428.1"
/db_xref="GeneID:854173"
CDS 344335..345798
/gene="TIR4"
/locus_tag="YOR009W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:10383953]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="Cell wall mannoprotein; expressed under anaerobic
conditions and required for anaerobic growth;
transcription is also induced by cold shock; member of the
Srp1p/Tip1p family of serine-alanine-rich proteins"
/codon_start=1
/product="Tir4p"
/protein_id="NP_014652.1"
/db_xref="GeneID:854173"
/db_xref="SGD:S000005535"
/translation="MAYSKITLLAALAAIAYAQTQAQINELNVVLDDVKTNIADYITL
SYTPNSGFSLDQMPAGIMDIAAQLVANPSDDSYTTLYSEVDFSAVEHMLTMVPWYSSR
LLPELEAMDASLTTSSSAATSSSEVASSSIASSTSSSVAPSSSEVVSSSVAPSSSEVV
SSSVAPSSSEVVSSSVASSSSEVASSSVAPSSSEVVSSSVASSSSEVASSSVAPSSSE
VVSSSVAPSSSEVVSSSVASSSSEVASSSVAPSSSEVVSSSVASSTSEATSSSAVTSS
SAVSSSTESVSSSSVSSSSAVSSSEAVSSSPVSSVVSSSAGPASSSVAPYNSTIASSS
STAQTSISTIAPYNSTTTTTPASSASSVIISTRNGTTVTETDNTLVTKETTVCDYSST
SAVPASTTGYNNSTKVSTATICSTCKEGTSTATDFSTLKTTVTVCDSACQAKKSATVV
SVQSKTTGIVEQTENGAAKAVIGMGAGALAAVAAMLL"
gene complement(<346195..>346950)
/gene="TIR2"
/locus_tag="YOR010C"
/gene_synonym="SRP2"
/db_xref="GeneID:854174"
mRNA complement(<346195..>346950)
/gene="TIR2"
/locus_tag="YOR010C"
/gene_synonym="SRP2"
/product="putative GPI-anchored mannoprotein"
/transcript_id="NM_001183429.1"
/db_xref="GeneID:854174"
CDS complement(346195..346950)
/gene="TIR2"
/locus_tag="YOR010C"
/gene_synonym="SRP2"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0071497 cellular
response to freezing [PMID:7746155]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="Putative cell wall mannoprotein; member of the
Srp1p/Tip1p family of serine-alanine-rich proteins;
transcription is induced by cold shock and anaerobiosis;
TIR2 has a paralog, TIR3, that arose from the whole genome
duplication"
/codon_start=1
/product="putative GPI-anchored mannoprotein"
/protein_id="NP_014653.1"
/db_xref="GeneID:854174"
/db_xref="SGD:S000005536"
/translation="MAYIKIALLAAIAALASAQTQEEIDELNVILNDVKSNLQEYISL
AEDSSSGFSLSSLPSGVLDIGLALASATDDSYTTLYSEVDFAAVSKMLTMVPWYSSRL
LPELESLLGTSTTAASSTEASSAATSSAVASSSETTSSAVASSSEATSSAVASSSEAS
SSAATSSAVASSSEATSSTVASSTKAASSTKASSSAVSSAVASSTKASAISQISDGQV
QATSTVSEQTENGAAKAVIGMGAGVMAAAAMLL"
gene <349679..>353863
/gene="AUS1"
/locus_tag="YOR011W"
/db_xref="GeneID:854175"
mRNA <349679..>353863
/gene="AUS1"
/locus_tag="YOR011W"
/product="ATP-binding cassette sterol transporter AUS1"
/transcript_id="NM_001183430.1"
/db_xref="GeneID:854175"
CDS 349679..353863
/gene="AUS1"
/locus_tag="YOR011W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:21521689]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0035376
sterol import [PMID:21110944|PMID:12077145]"
/experiment="EXISTENCE:mutant phenotype:GO:0034041
ABC-type sterol transporter activity [PMID:21521689]"
/experiment="EXISTENCE:mutant phenotype:GO:0035376 sterol
import [PMID:12077145|PMID:21521689]"
/note="Plasma membrane sterol transporter of the
ATP-binding cassette family; required, along with Pdr11p,
for uptake of exogenous sterols and their incorporation
into the plasma membrane; activity is stimulated by
phosphatidylserine; sterol uptake is required for
anaerobic growth because sterol biosynthesis requires
oxygen; AUS1 has a paralog, PDR11, that arose from the
whole genome duplication"
/codon_start=1
/product="ATP-binding cassette sterol transporter AUS1"
/protein_id="NP_014654.1"
/db_xref="GeneID:854175"
/db_xref="SGD:S000005537"
/translation="MSISKYFTPVADGSLTFNGANIQFGADAQGESKKSYDAEDSMPN
PANQLNDITFQAEAGEMVLVLGYPTSTLFKTLFHGKTSLSYSPPGSIKFKNNEFKSFS
EKCPHQIIYNNEQDVHFPFLTVEQTIDFALSCKFDIPKGERDQIRNELLREFGLSHVL
KTIVGNDFFRGVSGGERKRISIIETFIANGSVYLWDNSTKGLDSATALDFLEILRKMA
KATRSVNLVRISQASDKIVDKFDKILMLSDSYQLFYGTVDECLTYFRDTLGIEKDPND
CIIEYLTSILNFQFKNKNLGNLSNSSSASVLKTATGEVTKYTYNSDFDLYDQWKHSSY
YRNIKQQIQGSSIDDSIKEVDPSDVSPIFNIPLKKQLLFCTKRAFQRSLGDKAYMTAQ
FISVVIQSLVIGSLFYEIPLTTIGSYSRGSLTFFSILFFTFLSLADMPIAFQRQPVVK
KQSQLHFYTNWVETLSTTVFDYCFKLCLVIVFSIILYFLAHLQYKAARFFIFLLFLSF
YNFCMVSLFALTTLVAPTISVANLFAGILLLAIAMYASYVIYLKNMHPWFVWIAYLNP
AMYAMEAILSNELYNLKLDCSETIVPRGPTYNDVPFSHKACAWQGATLGNDYVRGRDY
LKQGLSYTYHHVWRNFGIIIGFLVFFIACTLFASQYIKPYFNKDEIERNNSRLTRWLP
FLNKKRGTRSSARNDSKYVGIPKSHSVSSSSSSLSAVPYQISPSNKEMALNDYNEQPI
TETVETQKHIISWKNINYTVGTKKLINNASGFISSGLTALMGESGAGKTTLLNVLSQR
VETGVVSGEILIDGHPLTDEDAFKRSIGFVQQQDLHLDLLSVKESLEISCLLRGDGDR
AYLDTVSNLLKLPSDILVADLNPTQRKLLSIGVELVTKPSLLLFLDEPTSGLDAEAAL
TIVKFLKQLSLQGQAIFCTIHQPSKSVISHFDNIFLLKRGGECVFFGPMDDACGYFMS
HDNTLVYDKEHDNPADFVIDAVGNSNSSAGKDTAEEALTLNKEAIDWSALWESSVEKK
LVKKETARLEDDARASGVDYTTSLWKQPSYLQQLALITRRQYICTKRDMTYVMAKYCL
NGGAGLFIGFSFWHIKHNIIGLQDSIFFCFMALCVSSPLINQIQDKALKTKEVYVARE
ARSNTYHWTVLLLSQSIIELPLALTSSTLFFVCAFFSCGFNNAGWSAGVFFLNYMLFA
AYYSTLGLWLIYTAPNLQTAAVFVAFIYSFTASFCGVMQPYSLFPTFWKFMYRVSPYT
YFVETFVSILLHNWEIKCDMSEMVPGQPLTGQSCGQFMEAFIEEYGGYLHNKNTFTVC
AYCTYTVGDDFLKNENMSYDHVWRNFGIEWAFVGFNFFAMFAGYYLTYVARIWPKVFK
IITKVIPHRGKKPVQN"
gene complement(354041..354113)
/locus_tag="YNCO0013C"
/db_xref="GeneID:854176"
tRNA complement(354041..354113)
/locus_tag="YNCO0013C"
/product="tRNA-Thr"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:854176"
/db_xref="SGD:S000006747"
repeat_region 354128..354466
/note="Ty3 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007159"
repeat_region 354681..354958
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007157"
gene <355652..>355858
/locus_tag="YOR011W-A"
/db_xref="GeneID:1466479"
mRNA <355652..>355858
/locus_tag="YOR011W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184576.1"
/db_xref="GeneID:1466479"
CDS 355652..355858
/locus_tag="YOR011W-A"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878167.1"
/db_xref="GeneID:1466479"
/db_xref="SGD:S000028581"
/translation="MAKSVFNFFHFEILEYLNRFVYHSQYFLPYYCSLEVLGKSRKNW
TFQYWCLYITTDKKIIKKKDFYHR"
gene <356543..>356956
/locus_tag="YOR012W"
/db_xref="GeneID:854177"
mRNA <356543..>356956
/locus_tag="YOR012W"
/product="uncharacterized protein"
/transcript_id="NM_001183431.1"
/db_xref="GeneID:854177"
CDS 356543..356956
/locus_tag="YOR012W"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014655.1"
/db_xref="GeneID:854177"
/db_xref="SGD:S000005538"
/translation="MVASSINEESSLAVNLTSDVEKASKTLFKAFEKSYANDYLMKKF
FHIPITEKVSRARINAMIHYYTTCYHDLDGEIAEANDFDAVAIWSRPGCHLPATLSDD
ESFNKIFFSRLDCEEARSHASGNGLLLPLCHRKRS"
gene <357674..>359947
/gene="RTS1"
/locus_tag="YOR014W"
/gene_synonym="SCS1"
/db_xref="GeneID:854179"
mRNA <357674..>359947
/gene="RTS1"
/locus_tag="YOR014W"
/gene_synonym="SCS1"
/product="protein phosphatase 2A regulatory subunit RTS1"
/transcript_id="NM_001183433.1"
/db_xref="GeneID:854179"
CDS 357674..359947
/gene="RTS1"
/locus_tag="YOR014W"
/gene_synonym="SCS1"
/experiment="EXISTENCE:direct assay:GO:0000776 kinetochore
[PMID:23785440|PMID:12636916]"
/experiment="EXISTENCE:direct assay:GO:0003677 DNA binding
[PMID:31781172]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922|PMID:12388751|PMID:12636916]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922|PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005816 spindle
pole body [PMID:12388751]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:12388751|PMID:12636916]"
/experiment="EXISTENCE:direct assay:GO:0019888 protein
phosphatase regulator activity [PMID:9079645]"
/experiment="EXISTENCE:genetic interaction:GO:0031134
sister chromatid biorientation [PMID:25236599]"
/experiment="EXISTENCE:genetic interaction:GO:0031578
mitotic spindle orientation checkpoint signaling
[PMID:19605686]"
/experiment="EXISTENCE:genetic interaction:GO:0032186
cellular bud neck septin ring organization
[PMID:12636916]"
/experiment="EXISTENCE:genetic interaction:GO:2000786
positive regulation of autophagosome assembly
[PMID:27973551]"
/experiment="EXISTENCE:mutant phenotype:GO:0006281 DNA
repair [PMID:31781172]"
/experiment="EXISTENCE:mutant phenotype:GO:0008104
intracellular protein localization [PMID:19605686]"
/experiment="EXISTENCE:mutant phenotype:GO:0031107 septin
ring disassembly [PMID:12636916]"
/experiment="EXISTENCE:mutant phenotype:GO:0032186
cellular bud neck septin ring organization
[PMID:12636916]"
/experiment="EXISTENCE:mutant phenotype:GO:0051754 meiotic
sister chromatid cohesion, centromeric [PMID:16541024]"
/experiment="EXISTENCE:mutant phenotype:GO:0070199
establishment of protein localization to chromosome
[PMID:24945276]"
/note="B-type regulatory subunit of protein phosphatase 2A
(PP2A); Rts1p and Cdc55p are alternative regulatory
subunits for PP2A catalytic subunits, Pph21p and Pph22p;
PP2A-Rts1p protects cohesin when recruited by Sgo1p to the
pericentromere; highly enriched at centromeres in the
absence of Cdc55p; required for maintenance of septin ring
organization during cytokinesis, for ring disassembly in
G1 and for dephosphorylation of septin, Shs1p; homolog of
the mammalian B' subunit of PP2A"
/codon_start=1
/product="protein phosphatase 2A regulatory subunit RTS1"
/protein_id="NP_014657.1"
/db_xref="GeneID:854179"
/db_xref="SGD:S000005540"
/translation="MMRGFKQRLIKKTTGSSSSSSSKKKDKEKEKEKSSTTSSTSKKP
ASASSSSHGTTHSSASSTGSKSTTEKGKQSGSVPSQGKHHSSSTSKTKTATTPSSSSS
SSRSSSVSRSGSSSTKKTSSRKGQEQSKQSQQPSQSQKQGSSSSSAAIMNPTPVLTVT
KDDKSTSGEDHAHPTLLGAVSAVPSSPISNASGTAVSSDVENGNSNNNNMNINTSNTQ
DANHASSQSIDIPRSSHSFERLPTPTKLNPDTDLELIKTPQRHSSSRFEPSRYTPLTK
LPNFNEVSPEERIPLFIAKVDQCNTMFDFNDPSFDIQGKEIKRSTLDELIEFLVTNRF
TYTNEMYAHVVNMFKINLFRPIPPPVNPVGDIYDPDEDEPVNELAWPHMQAVYEFFLR
FVESPDFNHQIAKQYIDQDFILKLLELFDSEDIRERDCLKTTLHRIYGKFLSLRSFIR
RSMNNIFLQFIYETEKFNGVAELLEILGSIINGFALPLKEEHKVFLVRILIPLHKVRC
LSLYHPQLAYCIVQFLEKDPLLTEEVVMGLLRYWPKINSTKEIMFLNEIEDIFEVIEP
LEFIKVEVPLFVQLAKCISSPHFQVAEKVLSYWNNEYFLNLCIENAEVILPIIFPALY
ELTSQLELDTANGEDSISDPYMLVEQAINSGSWNRAIHAMAFKALKIFLETNPVLYEN
CNALYLSSVKETQQRKVQREENWSKLEEYVKNLRINNDKDQYTIKNPELRNSFNTASE
NNTLNEENENDCDSEIQ"
gene <359993..>360352
/locus_tag="YOR015W"
/db_xref="GeneID:854180"
mRNA <359993..>360352
/locus_tag="YOR015W"
/product="uncharacterized protein"
/transcript_id="NM_001348876.1"
/db_xref="GeneID:854180"
CDS 359993..360352
/locus_tag="YOR015W"
/note="hypothetical protein; conserved among S. cerevisiae
strains; YOR015W is not an essential gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335814.1"
/db_xref="GeneID:854180"
/db_xref="SGD:S000005541"
/translation="MPHFKRAAVYEEQKRTGKWGQLVEETKDRIPEYSNKTIAKISHL
DNGCLWPEIKVSFSHHLSILQSMCLHFIISILFSKYIFVFLFAFLLPSAFPLFILHST
LFRKPCLSIIGFLKTKV"
gene complement(<360461..>361084)
/gene="ERP4"
/locus_tag="YOR016C"
/db_xref="GeneID:854181"
mRNA complement(<360461..>361084)
/gene="ERP4"
/locus_tag="YOR016C"
/product="Erp4p"
/transcript_id="NM_001183435.1"
/db_xref="GeneID:854181"
CDS complement(360461..361084)
/gene="ERP4"
/locus_tag="YOR016C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:10359606]"
/note="Member of the p24 family involved in ER to Golgi
transport; similar to Emp24p and Erv25p; ERP4 has a
paralog, ERP2, that arose from the whole genome
duplication"
/codon_start=1
/product="Erp4p"
/protein_id="NP_014659.1"
/db_xref="GeneID:854181"
/db_xref="SGD:S000005542"
/translation="MRVFTLIAILFSSSLLTHAFSSNYAPVGISLPAFTKECLYYDLS
SDKDVLVVSYQVLTGGNFEIDFDITAPDGSVIVTERQKKHSDFLLKSFGIGKYTFCLS
NNYGTSPKKVEITLEKEKEIVSSHESKEDIIANNAIEEIDRNLNKITKTMDYLRAREW
RNMYTVSSTESRLTWLSLLIMGVMVGISIVQALIIQFFFTSRQKNYV"
gene <361412..>363814
/gene="PET127"
/locus_tag="YOR017W"
/db_xref="GeneID:854182"
mRNA <361412..>363814
/gene="PET127"
/locus_tag="YOR017W"
/product="Pet127p"
/transcript_id="NM_001183436.1"
/db_xref="GeneID:854182"
CDS 361412..363814
/gene="PET127"
/locus_tag="YOR017W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005740
mitochondrial envelope [PMID:9111353]"
/experiment="EXISTENCE:mutant phenotype:GO:0000964
mitochondrial RNA 5'-end processing
[PMID:18086665|PMID:9111353]"
/note="Protein with a role in 5'-end processing of
mitochondrial RNAs; located in the mitochondrial membrane"
/codon_start=1
/product="Pet127p"
/protein_id="NP_014660.1"
/db_xref="GeneID:854182"
/db_xref="SGD:S000005543"
/translation="MGFYNCRFLSRRLSVEPCRIGSAAKSYQQRSIYHFGAALTNAPS
GRETPDKLRSDLHSALEMVDEIYDTNSTVEDIGNKEKGGRQKYTEEMDKAINLLKTNI
KKEYRHDKYLERTKVGTYPGRRTYPGRRTYPARRTYPASRTYSDSNSYTFRINVQKIR
HALVRYNQDGVQKHNQKPPRIGHGLTRVLYQPLSLQKLRDNRSRMYNFDPAVENINPE
YLEKKSEKDVNTDSSGEGQSKPIFITPHKDESLLKVAKEHRKKYISSSSSMTSVLSQL
HYLLSNFRRLNIIDSSISKNFPQKNCNYSESAYFPSAVILRKKRNGICSIDSDRSLDR
EIVLSVLGHYLEDFLTEKSLKNSSKSENYHYSSIDEFIVRSQLDAYDPNLPGTGVFDL
KTRAVSAIRYDLSHVESNNNQTGYEIDKVYGEFESLEREYFELIRSALLKYSLQARIG
KMDGIFVAYHNISKMFGFQYLPLDELDYIIHSSYNSKFDSLLKEKNDITKGIYGEEDY
ILHYDRDDRKIACLVANREFKMSMNLFSNILKHVEQLLNSSNTKWEKCKIMLKTEVEE
KRSKSGRFFNEPVLNIVALPLSPEYEDKSLLVKDTSNEQLTEELLNLRSYNENLLEEH
LNSLVGFKVNVKHFYHHHPNTTHLPDFALKKNDILDTESRKYISDMMKRDWYKDIPST
QTPNFFHASDVSTWEVNSTFTDINDKQILRKLYFKYLDVKLNALKNQVITRQEPDMSK
KDEIMNRIKSLQARNDHRDNGSNKRYSNFGPTRLQTKLRAYAKKGALRRKLLERSNKF
HI"
gene <364369..>366882
/gene="ROD1"
/locus_tag="YOR018W"
/gene_synonym="ART4"
/db_xref="GeneID:854183"
mRNA <364369..>366882
/gene="ROD1"
/locus_tag="YOR018W"
/gene_synonym="ART4"
/product="Rod1p"
/transcript_id="NM_001183437.1"
/db_xref="GeneID:854183"
CDS 364369..366882
/gene="ROD1"
/locus_tag="YOR018W"
/gene_synonym="ART4"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:8621680]"
/experiment="EXISTENCE:direct assay:GO:0031625 ubiquitin
protein ligase binding [PMID:24820415]"
/experiment="EXISTENCE:direct assay:GO:0071333 cellular
response to glucose stimulus [PMID:22249293]"
/experiment="EXISTENCE:genetic interaction:GO:0002092
positive regulation of receptor internalization
[PMID:24820415]"
/experiment="EXISTENCE:mutant phenotype:GO:0009410
response to xenobiotic stimulus [PMID:12163175]"
/experiment="EXISTENCE:mutant phenotype:GO:0031625
ubiquitin protein ligase binding [PMID:24820415]"
/experiment="EXISTENCE:mutant phenotype:GO:0070086
ubiquitin-dependent endocytosis [PMID:22249293]"
/experiment="EXISTENCE:physical interaction:GO:0031625
ubiquitin protein ligase binding [PMID:18976803]"
/note="Alpha-arrestin involved in ubiquitin-dependent
endocytosis; activating dephosphorylation relays glucose
signaling to transporter endocytosis; calcineurin
dephosphorylation is required for Rsp5p-dependent
internalization of agonist-occupied Ste2p, as part of
signal desensitization; recruits Rsp5p to Ste2p via its 2
PPXY motifs; protein abundance increases in response to
DNA replication stress; ROD1 has a paralog, ROG3, that
arose from the whole genome duplication"
/codon_start=1
/product="Rod1p"
/protein_id="NP_014661.1"
/db_xref="GeneID:854183"
/db_xref="SGD:S000005544"
/translation="MFSSSSRPSKEPLLFDIRLRNLDNDVLLIKGPPDEASSVLLSGT
IVLSITEPIQIKSLALRLFGRLRLNIPTVLQTVHGPHKRYSKFERNIYSHFWDDFNIK
SYFQNLYDNHNNGKITISSKSSTNLAALPKRKRALSTASLISSNGQTSASKNYHTLVK
GNYEFPFSAIIPGSLVESVEGLPNAAVTYALEATIERPKQPDLICKKHLRVIRTLAID
AVELSETVSVDNSWPEKVDYTISIPTKAIAIGSSTMINILIVPILKGLKLGPVRISLV
ENSQYCGSYGGVINQERMVAKLKLKDPLKHVAQIKKKRSLNEAADEGVDTDTGEFQDK
WEVRALLNIPASLTKCSQDCRILSNIKVRHKIKFTISLLNPDGHISELRAALPVQLFI
SPFVPVNVKTSDVIERTLKTFGPSYQVTSQHDNSFSSKNFVDDSEEDVIFQRSASALQ
LSSMPTIVSGSTLNINSTDAEATAVADTTMVTSLMVPPNYGNHVYDRVYGEVTNEDET
SASASSSAVESQAIHNIQNLYISDSNNSNNPILAPNPQIKIEDDSLNNCDSRGDSVNN
SNLNLVNSNLTISENWNNNSPSANRYNNIINAGLNSPSLTPSFAHLSRRNSYSRQTSS
TSLKNDLELTDLSRVPSYDKAMKSDMIGEDLPPAYPEEELGVQENKKIELERPQILHH
KSTSSLLPLPGSSKSSNNLKRSSSRTHLSHSPLPRNNSGSSVSLQQLARNNTDSSFNL
NLSFTSAKSSTGSRHFPFNMTTSFTSNSSSKNNSHFDKTDSTSDANKPREEENYTSAT
HNRRSRSSSVRSNNSNSPLRQGTGSFANLMEMFTKRDRS"
gene <368127..>370319
/locus_tag="YOR019W"
/db_xref="GeneID:854184"
mRNA <368127..>370319
/locus_tag="YOR019W"
/product="uncharacterized protein"
/transcript_id="NM_001183438.1"
/db_xref="GeneID:854184"
CDS 368127..370319
/locus_tag="YOR019W"
/experiment="EXISTENCE:mutant phenotype:GO:0000422
autophagy of mitochondrion [PMID:19793921]"
/note="hypothetical protein; may interact with ribosomes,
based on co-purification experiments; YOR019W has a
paralog, JIP4, that arose from the whole genome
duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014662.1"
/db_xref="GeneID:854184"
/db_xref="SGD:S000005545"
/translation="MISVCPQNDLQKCYRSLTFDVPGQQFEERNEQNLKKRAKKKGSF
QPSVAFDTVPSTAGYSSIDDSREGFKGVPVPNYYTMEECYDDETDSFSPNLQYYLRDT
FQSSPFLNTRKENKSESSSFPMRSSKLLEKNSDIKKYFLVSKNGKIVRRDYPSTPVIV
NETLMINRFEKNWIKLWRQRKLQINERLNDKKKWFTYPELIFSEERIKPLYRGDDSAP
CTKEQKRKHKILQQKVGYPNNPKTIVCHINGKKHTWVALDWTVYKFARNLDHIVVITT
LPKMISNRKKTAKDDTEWAPGYQKEVIDQKLNDIFDYILQLVKVVKISVKITLEIIVG
KIKKSLVDVINVHTPDFLVLATLKHERNENLITYKSKKLTDVFPVSYPIPTFVVPSKR
MYSFELNLQREVNEHYVSKNHMKHEHTDVESMSSSMFKKNTISDISSHISVDSYAEDF
KRQGYIKKQFNTSNDSIPRKLTGLAQHSRRKITGDIEKLQDDEKDRECTKEKLLLKKI
DIIIRESLKSSLAIETLPGKNVSQSSHGDQISSFKNALIGNGSKNTKFRKSLIPYSSS
EEQNTTTTIKLSSSPTSQIKFATSVKHKDGRAALGKARNLPDIRHSISFDKENSFDPS
DKSSSVDNSIPLRKVKSAGALRKVKTNDSSSSAGSKKSSSSFSTVNTFTGGGVGIFKV
FKSGSSSGNKSSSRRNSSSGDVFESDDRNDKKKKKKKKKKSLFLFGKI"
gene complement(<370524..>370844)
/gene="HSP10"
/locus_tag="YOR020C"
/gene_synonym="CPN10"
/db_xref="GeneID:854185"
mRNA complement(<370524..>370844)
/gene="HSP10"
/locus_tag="YOR020C"
/gene_synonym="CPN10"
/product="Hsp10p"
/transcript_id="NM_001183439.1"
/db_xref="GeneID:854185"
CDS complement(370524..370844)
/gene="HSP10"
/locus_tag="YOR020C"
/gene_synonym="CPN10"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:7903252]"
/experiment="EXISTENCE:direct assay:GO:0006457 protein
folding [PMID:7902576]"
/experiment="EXISTENCE:direct assay:GO:0042026 protein
refolding [PMID:9256426]"
/experiment="EXISTENCE:direct assay:GO:0051082 unfolded
protein binding [PMID:7902576]"
/experiment="EXISTENCE:mutant phenotype:GO:0042026 protein
refolding [PMID:9256426]"
/experiment="EXISTENCE:mutant phenotype:GO:0045041 protein
import into mitochondrial intermembrane space
[PMID:7913473]"
/experiment="EXISTENCE:mutant phenotype:GO:0051131
chaperone-mediated protein complex assembly
[PMID:7913473]"
/experiment="EXISTENCE:physical interaction:GO:0051087
protein-folding chaperone binding [PMID:9256426]"
/note="Mitochondrial matrix co-chaperonin; inhibits the
ATPase activity of Hsp60p, a mitochondrial chaperonin;
involved in protein folding and sorting in the
mitochondria; 10 kD heat shock protein with similarity to
E. coli groES"
/codon_start=1
/product="Hsp10p"
/protein_id="NP_014663.1"
/db_xref="GeneID:854185"
/db_xref="SGD:S000005546"
/translation="MSTLLKSAKSIVPLMDRVLVQRIKAQAKTASGLYLPEKNVEKLN
QAEVVAVGPGFTDANGNKVVPQVKVGDQVLIPQFGGSTIKLGNDDEVILFRDAEILAK
IAKD"
gene <371685..>371957
/gene="MCO10"
/locus_tag="YOR020W-A"
/db_xref="GeneID:1466480"
mRNA <371685..>371957
/gene="MCO10"
/locus_tag="YOR020W-A"
/product="Mco10p"
/transcript_id="NM_001184536.1"
/db_xref="GeneID:1466480"
CDS 371685..371957
/gene="MCO10"
/locus_tag="YOR020W-A"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0045259
proton-transporting ATP synthase complex [PMID:36882574]"
/experiment="EXISTENCE:genetic interaction:GO:0055074
calcium ion homeostasis [PMID:36882574]"
/experiment="EXISTENCE:mutant phenotype:GO:0055074 calcium
ion homeostasis [PMID:36882574]"
/note="Subunit l of the mitochondrial F1F0 ATP synthase;
F1F0 ATP synthase is a large, evolutionarily conserved
enzyme complex required for ATP synthesis; more abundant
in the ATP synthase monomer; involved in the
calcium-induced permeability transition and calcium
homeostasis; does not impact ATP synthase activity and
stability; N-terminal region has sequence similarity to
Atp19p; conserved in A. gossypii; detected in highly
purified mitochondria in high-throughput studies"
/codon_start=1
/product="Mco10p"
/protein_id="NP_878168.1"
/db_xref="GeneID:1466480"
/db_xref="SGD:S000028526"
/translation="MGAAYKVFGKTVQPHVLAISTFIATAAVASYFTTKPKTKNEGKN
SSALSQQKSGESSNSDAMGKDDDVVKSIEGFLNDLEKDTRQDTKAN"
gene complement(<372798..>373439)
/gene="SFM1"
/locus_tag="YOR021C"
/db_xref="GeneID:854186"
mRNA complement(<372798..>373439)
/gene="SFM1"
/locus_tag="YOR021C"
/product="protein-arginine N-methyltransferase SFM1"
/transcript_id="NM_001183440.1"
/db_xref="GeneID:854186"
CDS complement(372798..373439)
/gene="SFM1"
/locus_tag="YOR021C"
/experiment="EXISTENCE:direct assay:GO:0035241
protein-arginine omega-N monomethyltransferase activity
[PMID:22650761]"
/experiment="EXISTENCE:mutant phenotype:GO:0018216
peptidyl-arginine methylation [PMID:22650761]"
/note="SPOUT methyltransferase; catalyzes
omega-monomethylation of Rps3p on Arg-146; not an
essential gene; predicted to be involved in rRNA
processing and ribosome biogenesis and in biopolymer
catabolism"
/codon_start=1
/product="protein-arginine N-methyltransferase SFM1"
/protein_id="NP_014664.1"
/db_xref="GeneID:854186"
/db_xref="SGD:S000005547"
/translation="MKYIIEHMEEGFSEWVILEYSQILREVGAENLILSSLPESTTEK
DIPQRLLKLGLRWTTKDLKGINEDFKDLELLKDGRVCLLDPRATIDLQPEDATKFDYF
VFGGILGDHPPRDRTKELKTAYPNLLISRRLGDKQMTTDTAIRTTQLIIKDRIAFEDI
KFIDYPEFRFNKNEATEMPFRYVLDKEGKPILPEGMLDLIKKDSAQSLDDLLM"
gene complement(<373710..>375857)
/gene="DDL1"
/locus_tag="YOR022C"
/db_xref="GeneID:854187"
mRNA complement(<373710..>375857)
/gene="DDL1"
/locus_tag="YOR022C"
/product="putative carboxylic ester hydrolase"
/transcript_id="NM_001183441.1"
/db_xref="GeneID:854187"
CDS complement(373710..375857)
/gene="DDL1"
/locus_tag="YOR022C"
/experiment="EXISTENCE:direct assay:GO:0004620
glycerophospholipase activity
[PMID:27402848|PMID:27746179]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:27746179]"
/experiment="EXISTENCE:mutant phenotype:GO:0004620
glycerophospholipase activity [PMID:27402848]"
/experiment="EXISTENCE:mutant phenotype:GO:0006644
phospholipid metabolic process [PMID:27402848]"
/experiment="EXISTENCE:mutant phenotype:GO:0032048
cardiolipin metabolic process [PMID:27402848]"
/experiment="EXISTENCE:mutant phenotype:GO:0046337
phosphatidylethanolamine metabolic process
[PMID:27402848]"
/note="DDHD domain-containing phospholipase A1;
mitochondrial matrix enzyme with sn-1-specific activity,
hydrolyzing cardiolipin, PE, PC, PG and PA; implicated in
remodeling of mitochondrial phospholipids;
antagonistically regulated by Aft1p and Aft2p; in humans,
mutations in DDHD1 and DDHD2 genes cause specific types of
hereditary spastic paraplegia, while DDL1-defective yeast
share similar phenotypes such as mitochondrial dysfunction
and defects in lipid metabolism"
/codon_start=1
/product="putative carboxylic ester hydrolase"
/protein_id="NP_014665.1"
/db_xref="GeneID:854187"
/db_xref="SGD:S000005548"
/translation="MLRFTHRGLPSSTRFRNIFVRLNHIYVPWFYAIDVPNSKPYLPT
YQTLHSPKKFKPFSVDDSNRLEKASKRQERRPVLVNEDYLFKVDLSHMELSPTYWEGP
TYQVRRGVWFDSSNQPLSSDLTSEIEGLYKQLKFDDSNDDPTTTPPAESQDIFRLKGK
YPVDKENEGEQKNGSSNKDENESTFKFILFANKQTAFLLSDLDGGKLQLAFLRSNLAQ
SLPINATMITRSYKYSSSATTKQTSTSFKAAKTPQTEVADGSNSSKSRSIETKLEKKV
SNLFNLSDFLQLFNGNASKDQDDAQSLEKQMETDYNNADNSQGANASSKIEDGKNSGA
SDRQIRSNRRDVDNLILCVHGIGQTLGKKYEYVNFAHTVNLLRSNMKKIYNNSEKLQS
LNTAPDYKSNCNVQVLPITWRHSISFQTDAKEENIENPDLPTLSQVTVNGVLPLRKLL
ADGLLDILLYVEPYYQDMILQQVTSQLNKTYRIFKEFNPEFDGKVHLVGHSLGSMILF
DILSKQKKYELEFQVDNLFFIGSPIGLLKLIQRTKIGDRPEFPNDLERKLTVQRPQCK
DIYNVYHVCDPISYRMEPLVSKEMAHYEQTYLPHCSEAYGLTSKVLEFGENIWKDLPG
TDENNLQSKKTSPEKKEVKLSENLTRMLTGLNYTGRLDYAMSPSLLEVDFISAIKSHV
SYFEEPDIAAFILKEILSKHENASEIYVKRKTG"
gene complement(<376012..>377712)
/gene="AHC1"
/locus_tag="YOR023C"
/db_xref="GeneID:854188"
mRNA complement(<376012..>377712)
/gene="AHC1"
/locus_tag="YOR023C"
/product="Ahc1p"
/transcript_id="NM_001183442.1"
/db_xref="GeneID:854188"
CDS complement(376012..377712)
/gene="AHC1"
/locus_tag="YOR023C"
/experiment="EXISTENCE:direct assay:GO:0004402 histone
acetyltransferase activity [PMID:10490601]"
/experiment="EXISTENCE:direct assay:GO:0140671 ADA complex
[PMID:10490601]"
/experiment="EXISTENCE:mutant phenotype:GO:1990414
replication-born double-strand break repair via sister
chromatid exchange [PMID:23357952]"
/note="Subunit of the Ada histone acetyltransferase
complex; required for structural integrity of the complex;
Ahc2p and Ahc1p are unique to the ADA complex and not
shared with the related SAGA and SLIK complexes; Ahc2p may
tether Ahc1p to the complex"
/codon_start=1
/product="Ahc1p"
/protein_id="NP_014666.1"
/db_xref="GeneID:854188"
/db_xref="SGD:S000005549"
/translation="MMSPAQDKLQHQHHNPNSSSSSSSKMTNVYQVTTPKSPQDLENN
MDEPFKMDTATSNPDKDSENTQRLKYECAKGEIQNVLNLHIMLNHKHVRHLRRNVQKV
NAKLALLETLHKDTGLLNKIERTYQLKIKQHQQHSVLGGHFHDSTATENTNASNYNLS
YPVLSDYNINCQPLSSSSNRNLSTTRIPHHHYHTRSKSNGLLLEPSALRPANSNIIDY
RLTGSKSLSEAITKPTPVSLPHSNSDGISSPRSSSISPLDEQPGFQILPFKPSQMHLN
HRRNYSSTCLTSNSGIIGKTENNEPIFRRYDGILVIITCSKCDRSGFTSAQGIVNHTR
LKHSKLYSSQPLAVLNNQKLLPNDKQDPEILSKFKKLNLDPNKDYLPSDIAIPKPQSP
INHSENHTRAPKTVKNTPHLEKLYQNKEDFKKLIDMVNETPDDLNEYLKQREIQLRYQ
KEQEEESSKSDDEASYVPSPSLSATATTTTTTDPPSPPVLSSSLQRKLLRKRKLSLNS
STPMEDLPLRERLRANPTDKKPRKAALLTNELEGPDPAAKSSSYYNLRSKSRLRGSHT
"
gene <378219..>379562
/gene="HST3"
/locus_tag="YOR025W"
/db_xref="GeneID:854190"
mRNA <378219..>379562
/gene="HST3"
/locus_tag="YOR025W"
/product="NAD-dependent histone deacetylase HST3"
/transcript_id="NM_001183444.1"
/db_xref="GeneID:854190"
CDS 378219..379562
/gene="HST3"
/locus_tag="YOR025W"
/EC_number="2.3.1.286"
/experiment="EXISTENCE:curator inference:GO:0005634
nucleus [PMID:12242223]"
/experiment="EXISTENCE:direct assay:GO:0006351
DNA-templated transcription [PMID:31167142]"
/experiment="EXISTENCE:direct assay:GO:0009299 mRNA
transcription [PMID:31167142]"
/experiment="EXISTENCE:direct assay:GO:0017136 histone
deacetylase activity, NAD-dependent [PMID:17977840]"
/experiment="EXISTENCE:genetic interaction:GO:0031509
subtelomeric heterochromatin formation [PMID:7498786]"
/experiment="EXISTENCE:mutant phenotype:GO:0017136 histone
deacetylase activity, NAD-dependent [PMID:17977840]"
/experiment="EXISTENCE:mutant phenotype:GO:0046459
short-chain fatty acid metabolic process [PMID:12618394]"
/experiment="EXISTENCE:mutant phenotype:GO:1990414
replication-born double-strand break repair via sister
chromatid exchange [PMID:23357952]"
/note="Sirtuin, histone H3 deacetylase; involved along
with Hst4p in telomeric silencing, cell cycle progression,
radiation resistance, genomic stability and short-chain
fatty acid metabolism; member of the Sir2 family of
NAD(+)-dependent protein deacetylases"
/codon_start=1
/product="NAD-dependent histone deacetylase HST3"
/protein_id="NP_014668.1"
/db_xref="GeneID:854190"
/db_xref="SGD:S000005551"
/translation="MTSVSPSPPASRSGSMCSDLPSSLQTEKLAHIIGLDADDEVLRR
VTKQLSRSRRIACLTGAGISCNAGIPDFRSSDGLYDLVKKDCSQYWSIKSGREMFDIS
LFRDDFKISIFAKFMERLYSNVQLAKPTKTHKFIAHLKDRNKLLRCYTQNIDGLEESI
GLTLSNRKLPLTSFSSHWKNLDVVQLHGDLKTLSCTKCFQTFPWSRYWSRCLRRGELP
LCPDCEALINKRLNEGKRTLGSNVGILRPNIVLYGENHPSCEIITQGLNLDIIKGNPD
FLIIMGTSLKVDGVKQLVKKLSKKIHDRGGLIILVNKTPIGESSWHGIIDYQIHSDCD
NWVTFLESQIPDFFKTQDQIKKLRQLKREASDLRKQMKAQKDSIGTPPTTPLRTAQGI
DIQGNNELNTKIKSLNTVKRKILSPENSSEEDEEENLDTRKRAKIRPTFGDNQAS"
gene <379781..>380806
/gene="BUB3"
/locus_tag="YOR026W"
/gene_synonym="PAC9"
/db_xref="GeneID:854191"
mRNA <379781..>380806
/gene="BUB3"
/locus_tag="YOR026W"
/gene_synonym="PAC9"
/product="Bub3p"
/transcript_id="NM_001183445.1"
/db_xref="GeneID:854191"
CDS 379781..380806
/gene="BUB3"
/locus_tag="YOR026W"
/gene_synonym="PAC9"
/experiment="EXISTENCE:direct assay:GO:0000776 kinetochore
[PMID:27170178]"
/experiment="EXISTENCE:direct assay:GO:0033597 mitotic
checkpoint complex [PMID:11726501]"
/experiment="EXISTENCE:direct assay:GO:0043130 ubiquitin
binding [PMID:21070969]"
/experiment="EXISTENCE:direct assay:GO:1902499 positive
regulation of protein autoubiquitination [PMID:22940250]"
/experiment="EXISTENCE:genetic interaction:GO:0044774
mitotic DNA integrity checkpoint signaling
[PMID:16651657]"
/experiment="EXISTENCE:physical interaction:GO:0033597
mitotic checkpoint complex [PMID:11726501|PMID:10704439]"
/note="Kinetochore checkpoint WD40 repeat protein;
localizes to kinetochores during prophase and metaphase,
delays anaphase in the presence of unattached
kinetochores; forms complexes with Mad1p-Bub1p and with
Cdc20p, binds Mad2p and Mad3p; functions at kinetochore to
activate APC/C-Cdc20p for normal mitotic progression"
/codon_start=1
/product="Bub3p"
/protein_id="NP_014669.1"
/db_xref="GeneID:854191"
/db_xref="SGD:S000005552"
/translation="MQIVQIEQAPKDYISDIKIIPSKSLLLITSWDGSLTVYKFDIQA
KNVDLLQSLRYKHPLLCCNFIDNTDLQIYVGTVQGEILKVDLIGSPSFQALTNNEANL
GICRICKYGDDKLIAASWDGLIEVIDPRNYGDGVIAVKNLNSNNTKVKNKIFTMDTNS
SRLIVGMNNSQVQWFRLPLCEDDNGTIEESGLKYQIRDVALLPKEQEGYACSSIDGRV
AVEFFDDQGDDYNSSKRFAFRCHRLNLKDTNLAYPVNSIEFSPRHKFLYTAGSDGIIS
CWNLQTRKKIKNFAKFNEDSVVKIACSDNILCLATSDDTFKTNAAIDQTIELNASSIY
IIFDYEN"
gene <381053..>382822
/gene="STI1"
/locus_tag="YOR027W"
/db_xref="GeneID:854192"
mRNA <381053..>382822
/gene="STI1"
/locus_tag="YOR027W"
/product="Hsp90 cochaperone STI1"
/transcript_id="NM_001183446.1"
/db_xref="GeneID:854192"
CDS 381053..382822
/gene="STI1"
/locus_tag="YOR027W"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:21124907]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:39739753]"
/experiment="EXISTENCE:direct assay:GO:0030544 Hsp70
protein binding [PMID:12716905]"
/experiment="EXISTENCE:direct assay:GO:0042030 ATPase
inhibitor activity [PMID:21170051]"
/experiment="EXISTENCE:direct assay:GO:0051879 Hsp90
protein binding [PMID:9927435|PMID:12525481]"
/experiment="EXISTENCE:mutant phenotype:GO:0006457 protein
folding [PMID:8972212]"
/experiment="EXISTENCE:mutant phenotype:GO:0006626 protein
targeting to mitochondrion [PMID:27412066]"
/experiment="EXISTENCE:mutant phenotype:GO:0008104
intracellular protein localization [PMID:24109600]"
/experiment="EXISTENCE:physical interaction:GO:0005737
cytoplasm [PMID:12525481]"
/note="Evolutionarily-conserved Hsp90 cochaperone;
regulates spatial organization of amyloid-like proteins in
the cytosol, thereby buffering the proteotoxicity caused
by amyloid-like proteins; interacts with the Ssa group of
the cytosolic Hsp70 chaperones and activates Ssa1p ATPase
activity; interacts with Hsp90 chaperones and inhibits
their ATPase activity; homolog of mammalian Hop"
/codon_start=1
/product="Hsp90 cochaperone STI1"
/protein_id="NP_014670.1"
/db_xref="GeneID:854192"
/db_xref="SGD:S000005553"
/translation="MSLTADEYKQQGNAAFTAKDYDKAIELFTKAIEVSETPNHVLYS
NRSACYTSLKKFSDALNDANECVKINPSWSKGYNRLGAAHLGLGDLDEAESNYKKALE
LDASNKAAKEGLDQVHRTQQARQAQPDLGLTQLFADPNLIENLKKNPKTSEMMKDPQL
VAKLIGYKQNPQAIGQDLFTDPRLMTIMATLMGVDLNMDDINQSNSMPKEPETSKSTE
QKKDAEPQSDSTTSKENSSKAPQKEESKESEPMEVDEDDSKIEADKEKAEGNKFYKAR
QFDEAIEHYNKAWELHKDITYLNNRAAAEYEKGEYETAISTLNDAVEQGREMRADYKV
ISKSFARIGNAYHKLGDLKKTIEYYQKSLTEHRTADILTKLRNAEKELKKAEAEAYVN
PEKAEEARLEGKEYFTKSDWPNAVKAYTEMIKRAPEDARGYSNRAAALAKLMSFPEAI
ADCNKAIEKDPNFVRAYIRKATAQIAVKEYASALETLDAARTKDAEVNNGSSAREIDQ
LYYKASQQRFQPGTSNETPEETYQRAMKDPEVAAIMQDPVMQSILQQAQQNPAALQEH
MKNPEVFKKIQTLIAAGIIRTGR"
gene complement(<383533..>384420)
/gene="CIN5"
/locus_tag="YOR028C"
/gene_synonym="HAL6; YAP4"
/db_xref="GeneID:854193"
mRNA complement(<383533..>384420)
/gene="CIN5"
/locus_tag="YOR028C"
/gene_synonym="HAL6; YAP4"
/product="Cin5p"
/transcript_id="NM_001183447.1"
/db_xref="GeneID:854193"
CDS complement(383533..384420)
/gene="CIN5"
/locus_tag="YOR028C"
/gene_synonym="HAL6; YAP4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11179441|PMID:19029946]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:19029946]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding
[PMID:19111667|PMID:9372930|PMID:20802496]"
/experiment="EXISTENCE:direct assay:GO:0140297 DNA-binding
transcription factor binding [PMID:21552514]"
/experiment="EXISTENCE:genetic interaction:GO:0006972
hyperosmotic response [PMID:19774548]"
/experiment="EXISTENCE:mutant phenotype:GO:0009410
response to xenobiotic stimulus
[PMID:9593122|PMID:11179441|PMID:15821130]"
/experiment="EXISTENCE:mutant phenotype:GO:0042538
hyperosmotic salinity response [PMID:9559673]"
/note="Basic leucine zipper (bZIP) transcription factor of
the yAP-1 family; physically interacts with the Tup1-Cyc8
complex and recruits Tup1p to its targets; mediates
pleiotropic drug resistance and salt tolerance; nuclearly
localized under oxidative stress and sequestered in the
cytoplasm by Lot6p under reducing conditions; CIN5 has a
paralog, YAP6, that arose from the whole genome
duplication"
/codon_start=1
/product="Cin5p"
/protein_id="NP_014671.1"
/db_xref="GeneID:854193"
/db_xref="SGD:S000005554"
/translation="MLMQIKMDNHPFNFQPILASHSMTRDSTKPKKMTDTAFVPSPPV
GFIKEENKADLHTISVVASNVTLPQIQLPKIATLEEPGYESRTGSLTDLSGRRNSVNI
GALCEDVPNTAGPHIARPVTINNLIPPSLPRLNTYQLRPQLSDTHLNCHFNSNPYTTA
SHAPFESSYTTASTFTSQPAASYFPSNSTPATRKNSATTNLPSEERRRVSVSLSEQVF
NEGERYNNDGQLIGKTGKPLRNTKRAAQNRSAQKAFRQRREKYIKNLEEKSKLFDGLM
KENSELKKMIESLKSKLKE"
gene <384600..>384935
/locus_tag="YOR029W"
/db_xref="GeneID:854194"
mRNA <384600..>384935
/locus_tag="YOR029W"
/product="uncharacterized protein"
/transcript_id="NM_001348877.1"
/db_xref="GeneID:854194"
CDS 384600..384935
/locus_tag="YOR029W"
/note="hypothetical protein; conserved among S. cerevisiae
strains; YOR029W is not an essential gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335815.1"
/db_xref="GeneID:854194"
/db_xref="SGD:S000005555"
/translation="MMQTSTSSRVRRYPYQITLSLVLKGFYSPSAPSYDFCLVLLPTL
FLIDLMPIKFSLHVTIGIGEATPVPIFFFSAPWYFRSGNPLPHCVRAYRCKVNFPFFR
LGWSTWLHY"
gene <386825..>388684
/gene="DFG16"
/locus_tag="YOR030W"
/gene_synonym="ECM41; ZRG12"
/db_xref="GeneID:854195"
mRNA <386825..>388684
/gene="DFG16"
/locus_tag="YOR030W"
/gene_synonym="ECM41; ZRG12"
/product="Dfg16p"
/transcript_id="NM_001183449.1"
/db_xref="GeneID:854195"
CDS 386825..388684
/gene="DFG16"
/locus_tag="YOR030W"
/gene_synonym="ECM41; ZRG12"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:23019326]"
/experiment="EXISTENCE:mutant phenotype:GO:0007124
pseudohyphal growth [PMID:9055077]"
/experiment="EXISTENCE:mutant phenotype:GO:0016485 protein
processing [PMID:15879523]"
/note="Probable multiple transmembrane protein; involved
in diploid invasive and pseudohyphal growth upon nitrogen
starvation; is glycosylated and phosphorylated; interacts
with Rim21p and Rim9p in the plasma membrane to form a
pH-sensing complex in the Rim101 pathway and is required
to maintain Rim21p levels; required for accumulation of
processed Rim101p"
/codon_start=1
/product="Dfg16p"
/protein_id="NP_014673.1"
/db_xref="GeneID:854195"
/db_xref="SGD:S000005556"
/translation="MIIRLHFYYLLTLVYHLGLVGAYEKAARKRIQPPDLIPGPPGHK
LGDERPPHYDHRPPYKKHIDNIPAYNLTDLIDDKLLNKYENSCTVNVLTGGFISLASN
SWHLRAYNYTLNYPSFLIRCDNGSANPNFSHVLQDFVYDINNKFNVQDDSSKYIGKDP
FPLGMIMITFASGCICVATWMLFLVVLLLPSDNHNRRNKVVHVYVLFSAIIRTVFLNE
TIAVIFDSQYHDDYQDASQFESFIVETAPYKICELVANILSDINWIYIVHYLQSNYGK
PTWNWIPFKMKKGTHIIITVGCFLSLADNILFANLLWRKNLVVLKVFYKLIELLIYTI
FISIICYFTWHNFAYILLPKTAEINTDGKCKTKLRILWENYHETIPLLAYNILIFILF
YFTTIFFAAFTKHVRGWTFNFVHLLKVLITVNVWGLIGVLEKRELHISKKTVLGRKIN
NRDKFFANPTVNYYGEDLGKHLSAITLNRDLNTTKSNTTSHDSSSLVGSPSPTWKSPI
ERIRDRRRRHKIMKSENKFGQNPSFGSKSNGKPNTKTTLSKYRQLLRKPRRKTNSYEP
KNGIGQNKEGSTVRPGADKHIRDSNYLATDISDNESMETELRTNHIYNYENSD"
gene 389213..389422
/gene="CRS5"
/locus_tag="YOR031W"
/pseudo
/db_xref="GeneID:854196"
CDS 389213..389422
/gene="CRS5"
/locus_tag="YOR031W"
/experiment="EXISTENCE:direct assay:GO:0005507 copper ion
binding [PMID:17163970]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:7929222]"
/experiment="EXISTENCE:mutant phenotype:GO:0005507 copper
ion binding [PMID:7929222]"
/experiment="EXISTENCE:mutant phenotype:GO:0010038
response to metal ion [PMID:17163970|PMID:7929222]"
/note="Copper-binding metallothionein; required for
wild-type copper resistance"
/pseudo
/codon_start=1
/db_xref="GeneID:854196"
/db_xref="SGD:S000005557"
gene complement(<389771..>391075)
/gene="HMS1"
/locus_tag="YOR032C"
/db_xref="GeneID:854197"
mRNA complement(<389771..>391075)
/gene="HMS1"
/locus_tag="YOR032C"
/product="Hms1p"
/transcript_id="NM_001183451.1"
/db_xref="GeneID:854197"
CDS complement(389771..391075)
/gene="HMS1"
/locus_tag="YOR032C"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:39756762]"
/experiment="EXISTENCE:genetic interaction:GO:0007124
pseudohyphal growth [PMID:9832522]"
/note="Core promoter binding basic helix-loop-helix bHLH
protein; overexpression confers hyperfilamentous growth
and suppresses the pseudohyphal filamentation defect of a
diploid mep1 mep2 homozygous null mutant; similarity to
myc-family transcription factors"
/codon_start=1
/product="Hms1p"
/protein_id="NP_014675.1"
/db_xref="GeneID:854197"
/db_xref="SGD:S000005558"
/translation="MPNFQKPFSGSSDGNSVMNDLGNKVAIKVFDCRSAQDGSEEQNV
NVTTNQMYLMFQSNNYNVPPPNYNTEDLGSQGPPTHAYYAPFQHPIHLQPPVPPVYKN
NTYSATDQYSDSSFPNTSGHTPVIDSNYYNDALASIPTTTTGSTTMTTDNGNTIDSEE
YIDNMEVFSSEENENIDNVKQTDLKSEKDSSLLSAASIVKKEQLSGFENFLPLSKTES
PLVTADEIKSSLNLENIDNADSMSFKLKTSPIRKHFHVKPKRITRVRTGRVSHNIIEK
KYRSNINDKIEQLRRTVPTLRVAYKKCNDLPITSRDLADLDGLEPATKLNKASILTKS
IEYICHLERKCLQLSLANQHLSNDTRDSFVHLTEPSQPLSDNSSSEQVQKQTRSCQRQ
RQRQPRQQQPLHNIQYNIPHQNGLMSGTNNSHDMDFNNAGDF"
gene <392176..>392376
/locus_tag="YOR032W-A"
/db_xref="GeneID:1466481"
mRNA <392176..>392376
/locus_tag="YOR032W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184624.1"
/db_xref="GeneID:1466481"
CDS 392176..392376
/locus_tag="YOR032W-A"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/note="hypothetical protein; SWAT-GFP and seamless-GFP
fusion proteins localize to the endoplasmic reticulum;
identified by gene-trapping, microarray-based expression
analysis, and genome-wide homology searching"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878169.1"
/db_xref="GeneID:1466481"
/db_xref="SGD:S000028710"
/translation="MRRALFIAGQTYLWLNLTHLLLIFSWSSTMAFSQSRRLLTPTVP
CPTLLGIDFLILVLRHFDEIFI"
gene complement(<392416..>394524)
/gene="EXO1"
/locus_tag="YOR033C"
/gene_synonym="DHS1"
/db_xref="GeneID:854198"
mRNA complement(<392416..>394524)
/gene="EXO1"
/locus_tag="YOR033C"
/gene_synonym="DHS1"
/product="Rad2 family nuclease EXO1"
/transcript_id="NM_001183452.1"
/db_xref="GeneID:854198"
CDS complement(392416..394524)
/gene="EXO1"
/locus_tag="YOR033C"
/gene_synonym="DHS1"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0008409 5'-3'
exonuclease activity [PMID:12531018]"
/experiment="EXISTENCE:direct assay:GO:0017108 5'-flap
endonuclease activity [PMID:12531018]"
/experiment="EXISTENCE:genetic interaction:GO:0000706
meiotic DNA double-strand break processing
[PMID:20150422]"
/experiment="EXISTENCE:genetic interaction:GO:0000723
telomere maintenance [PMID:15126387|PMID:19595717]"
/experiment="EXISTENCE:genetic interaction:GO:0000729 DNA
double-strand break processing
[PMID:18805091|PMID:17347674|PMID:21841787]"
/experiment="EXISTENCE:genetic interaction:GO:0031860
telomeric 3' overhang formation [PMID:20808892]"
/experiment="EXISTENCE:genetic interaction:GO:0044818
mitotic G2/M transition checkpoint [PMID:18805091]"
/experiment="EXISTENCE:mutant phenotype:GO:0000729 DNA
double-strand break processing [PMID:17347674]"
/experiment="EXISTENCE:mutant phenotype:GO:0006298
mismatch repair [PMID:11438669]"
/experiment="EXISTENCE:mutant phenotype:GO:0007534 gene
conversion at mating-type locus [PMID:18806779]"
/note="5'-3' exonuclease and flap-endonuclease; involved
in recombination, double-strand break repair, MMS2
error-free branch of the post replication (PRR) pathway
and DNA mismatch repair; role in telomere maintenance;
member of the Rad2p nuclease family, with conserved N and
I nuclease domains; relative distribution to the nucleus
increases upon DNA replication stress; EXO1 has a paralog,
DIN7, that arose from the whole genome duplication"
/codon_start=1
/product="Rad2 family nuclease EXO1"
/protein_id="NP_014676.1"
/db_xref="GeneID:854198"
/db_xref="SGD:S000005559"
/translation="MGIQGLLPQLKPIQNPVSLRRYEGEVLAIDGYAWLHRAACSCAY
ELAMGKPTDKYLQFFIKRFSLLKTFKVEPYLVFDGDAIPVKKSTESKRRDKRKENKAI
AERLWACGEKKNAMDYFQKCVDITPEMAKCIICYCKLNGIRYIVAPFEADSQMVYLEQ
KNIVQGIISEDSDLLVFGCRRLITKLNDYGECLEICRDNFIKLPKKFPLGSLTNEEII
TMVCLSGCDYTNGIPKVGLITAMKLVRRFNTIERIILSIQREGKLMIPDTYINEYEAA
VLAFQFQRVFCPIRKKIVSLNEIPLYLKDTESKRKRLYACIGFVIHRETQKKQIVHFD
DDIDHHLHLKIAQGDLNPYDFHQPLANREHKLQLASKSNIEFGKTNTTNSEAKVKPIE
SFFQKMTKLDHNPKVANNIHSLRQAEDKLTMAIKRRKLSNANVVQETLKDTRSKFFNK
PSMTVVENFKEKGDSIQDFKEDTNSQSLEEPVSESQLSTQIPSSFITTNLEDDDNLSE
EVSEVVSDIEEDRKNSEGKTIGNEIYNTDDDGDGDTSEDYSETAESRVPTSSTTSFPG
SSQRSISGCTKVLQKFRYSSSFSGVNANRQPLFPRHVNQKSRGMVYVNQNRDDDCDDN
DGKNQITQRPSLRKSLIGARSQRIVIDMKSVDERKSFNSSPILHEESKKRDIETTKSS
QARPAVRSISLLSQFVYKGK"
gene complement(<394837..>397086)
/gene="AKR2"
/locus_tag="YOR034C"
/db_xref="GeneID:854199"
mRNA complement(<394837..>397086)
/gene="AKR2"
/locus_tag="YOR034C"
/product="putative palmitoyltransferase AKR2"
/transcript_id="NM_001183453.1"
/db_xref="GeneID:854199"
CDS complement(394837..397086)
/gene="AKR2"
/locus_tag="YOR034C"
/EC_number="2.3.1.225"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:19001347]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:16647879]"
/note="Ankyrin repeat-containing protein; member of a
family of putative palmitoyltransferases containing an
Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly
involved in constitutive endocytosis of Ste3p; AKR2 has a
paralog, AKR1, that arose from the whole genome
duplication"
/codon_start=1
/product="putative palmitoyltransferase AKR2"
/protein_id="NP_014677.1"
/db_xref="GeneID:854199"
/db_xref="SGD:S000005560"
/translation="MTSMSIIDDENVKKTSNGAAVVTDVAQHAVSDSDNNKAQLLGDG
SNTEYVVDIFIEAAKDGDLKVVKDVVESGAVDINNDRIDELSGLHWACINNRFSVAKF
LLLRGANPNQAAGPGGATALHWAARYGNIYIVDLLLKHGADPTLKDEQGLNIMHFSVY
SSNILLVVYVLYFVVNNNDNVDIDSKDNNNRTPLLWAAYQGDFLTVELLLKFGSTVAW
TDNRGFNALHCALVGGDQRVICDLILSGANFYERNNQKQDCFDLAEGMGTKSLFEQAL
QHHGYDRLGNQKDKLFKKSSHAQFTIFLSPFLLMVYIYLISLVLSPVLAIMLSLLVTV
VMVNTLKKFVLPCLPRKNTYKVSLTRTPFFSGLFLSTFCFLIYIWTKKLYPYSVSDYT
MKNVQFLVTSFLTVVLFLRLVRSDPGCLKTDDSLTSIQETIKQLIDLGKFDRENFCVE
TLERKPLRSKYSFFSGALVARYDHYCPWIYNDVGLKNHKLFVFFAVTVQYHMFLFMWL
CLAYFKKTNYIYEQVEEYARCALLKNETLCKGSNYDPSTFFLFIWISVNFIWLGAMLI
VQFFQILKGITTPELFILIKEEHKAKFINLIPFENSIYTSESKGVEDSDMIPEGPSAT
TITHTISIDGLEPRNRRRAILSACFSMMGINQWLVTIKEIVGITHILHGQVPQQHHSS
LLRSFLVTNHWKTNLTDFWLNSDVTAPLWQRFFYSSDTSKAMLGGTEVDYYELYEYPA
REGEVLRPN"
gene complement(<397426..>397668)
/locus_tag="YOR034C-A"
/db_xref="GeneID:1466482"
mRNA complement(<397426..>397668)
/locus_tag="YOR034C-A"
/product="uncharacterized protein"
/transcript_id="NM_001184667.1"
/db_xref="GeneID:1466482"
CDS complement(397426..397668)
/locus_tag="YOR034C-A"
/note="hypothetical protein; identified by expression
profiling and mass spectrometry"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878170.1"
/db_xref="GeneID:1466482"
/db_xref="SGD:S000028856"
/translation="MNTQELCKIFVAREYPLVVVPFIYFVLFLHQKYHTTLNYVWYPT
CSKRIWVREKGRKCSFFFFSKVPRSDGFANNRCQRK"
gene complement(<397735..>400104)
/gene="SHE4"
/locus_tag="YOR035C"
/gene_synonym="DIM1"
/db_xref="GeneID:854200"
mRNA complement(<397735..>400104)
/gene="SHE4"
/locus_tag="YOR035C"
/gene_synonym="DIM1"
/product="She4p"
/transcript_id="NM_001183454.1"
/db_xref="GeneID:854200"
CDS complement(397735..400104)
/gene="SHE4"
/locus_tag="YOR035C"
/gene_synonym="DIM1"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:8625407]"
/experiment="EXISTENCE:direct assay:GO:0017022 myosin
binding [PMID:12725728]"
/experiment="EXISTENCE:mutant phenotype:GO:0007533 mating
type switching [PMID:8625407]"
/experiment="EXISTENCE:mutant phenotype:GO:0008298
intracellular mRNA localization [PMID:11553699]"
/note="Protein containing a UCS (UNC-45/CRO1/SHE4) domain;
binds to myosin motor domains to regulate myosin function;
involved in endocytosis, polarization of the actin
cytoskeleton, and asymmetric mRNA localization"
/codon_start=1
/product="She4p"
/protein_id="NP_014678.1"
/db_xref="GeneID:854200"
/db_xref="SGD:S000005561"
/translation="MPLCEKGNDPIDSSTIDSLCAAFDKTLKSTPDVQKYNDAINTIF
QLRQKSESGKMPADLTNSEALKDRQKIEEILTRSYQDHSESRVHLSKLIQNDIPFALN
LFEILSRSSIHVFVGCFSNKDATIALLNELQIRIHYGEDTHVTYLLSIILQLLNKFKY
NFKEVRFLVKELILRISEDEVKSMMLIIFAELQSSFQKDFDKAVVDFMSSLIVEAEID
VGNDPLSIIVKTLSELYPSLTTLCSEIFLTKGLSKLFKKRVFEEQDLQFTKELLRLLS
SACIDETMRTYITENYLQLLERSLNVEDVQIYSALVLVKTWSFTKLTCINLKQLSEIF
INAISRRIMPKIENVNESAVKLEEVPKVEMSVEALAYLSLKASVKIMIRSNESFTEIL
LTMIKSQKMTHCLYGLLVIMANLSTLPEESNGSSQSINDLKNYADLKGPGADKVGAEK
ESKEDILLFNEKYILRTELISFLKREMHNLSPNCKQQVVRVIYNITRSKNFIPQCISQ
GGTTIILEYLANKQDIGEPIRILGCRALTRMLIFTNPGLIFKKYSALNAIPFLFELLP
RSTPVDDNPLHNDEQIKLTDNYEALLALTNLASSETSDGEEVCKHIVSTKVYWSTIEN
LMLDENVPLQRSTLELISNMMSHPLTIAAKFFNLENPQSLRNFNILVKLLQLSDVESQ
RAVAAIFANIATTIPLIAKELLTKKELIENAIQVFADQIDDIELRQRLLMLFFGLFEV
IPDNGTNEVYPLLQENQKLKDALNMSLKRGDSGPEFSAAIPVILAKIKV"
gene <400348..>401214
/gene="PEP12"
/locus_tag="YOR036W"
/gene_synonym="VPL6; VPS6; VPT13"
/db_xref="GeneID:854201"
mRNA <400348..>401214
/gene="PEP12"
/locus_tag="YOR036W"
/gene_synonym="VPL6; VPS6; VPT13"
/product="SNAP receptor PEP12"
/transcript_id="NM_001183455.1"
/db_xref="GeneID:854201"
CDS 400348..401214
/gene="PEP12"
/locus_tag="YOR036W"
/gene_synonym="VPL6; VPS6; VPT13"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005484 SNAP
receptor activity [PMID:14981247]"
/experiment="EXISTENCE:mutant phenotype:GO:0000011 vacuole
inheritance [PMID:1493335]"
/experiment="EXISTENCE:mutant phenotype:GO:0005484 SNAP
receptor activity [PMID:8730101]"
/experiment="EXISTENCE:mutant phenotype:GO:0005768
endosome [PMID:8730101]"
/experiment="EXISTENCE:mutant phenotype:GO:0005794 Golgi
apparatus [PMID:8730101]"
/experiment="EXISTENCE:mutant phenotype:GO:0006896 Golgi
to vacuole transport [PMID:8730101]"
/experiment="EXISTENCE:mutant phenotype:GO:0016236
macroautophagy [PMID:19793921]"
/experiment="EXISTENCE:mutant phenotype:GO:0032258
cytoplasm to vacuole targeting by the Cvt pathway
[PMID:19793921]"
/note="Target membrane receptor (t-SNARE); for vesicular
intermediates traveling between the Golgi apparatus and
the vacuole; controls entry of biosynthetic, endocytic,
and retrograde traffic into the prevacuolar compartment;
syntaxin"
/codon_start=1
/product="SNAP receptor PEP12"
/protein_id="NP_014679.1"
/db_xref="GeneID:854201"
/db_xref="SGD:S000005562"
/translation="MSEDEFFGGDNEAVWNGSRFSDSPEFQTLKEEVAAELFEINGQI
STLQQFTATLKSFIDRGDVSAKVVERINKRSVAKIEEIGGLIKKVNTSVKKMDAIEEA
SLDKTQIIAREKLVRDVSYSFQEFQGIQRQFTQVMKQVNERAKESLEASEMANDAALL
DEEQRQNSSKSTRIPGSQIVIERDPINNEEFAYQQNLIEQRDQEISNIERGITELNEV
FKDLGSVVQQQGVLVDNIEANIYTTSDNTQLASDELRKAMRYQKRTSRWRVYLLIVLL
VMLLFIFLIMKL"
gene <401555..>402655
/gene="CYC2"
/locus_tag="YOR037W"
/db_xref="GeneID:854202"
mRNA <401555..>402655
/gene="CYC2"
/locus_tag="YOR037W"
/product="oxidoreductase"
/transcript_id="NM_001183456.1"
/db_xref="GeneID:854202"
CDS 401555..402655
/gene="CYC2"
/locus_tag="YOR037W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:11914276|PMID:16823961|PMID:14576278|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:16207709]"
/experiment="EXISTENCE:direct assay:GO:0016491
oxidoreductase activity [PMID:16207709]"
/experiment="EXISTENCE:genetic interaction:GO:0018063
cytochrome c-heme linkage [PMID:16207709|PMID:14514677]"
/experiment="EXISTENCE:mutant phenotype:GO:0007006
mitochondrial membrane organization [PMID:11488609]"
/note="Mitochondrial peripheral inner membrane protein;
contains a FAD cofactor in a domain exposed in the
intermembrane space; exhibits redox activity in vitro;
likely participates in ligation of heme to acytochromes c
and c1 (Cyc1p and Cyt1p)"
/codon_start=1
/product="oxidoreductase"
/protein_id="NP_014680.2"
/db_xref="GeneID:854202"
/db_xref="SGD:S000005563"
/translation="MLWKNYVLSSSRITRRLHKSPRKSSFSKNFFITGCLLTVGAVSS
YLTYRYTSERENKHELSPSYFVKYKISHKRDIDSSHFLLEVTPLFKQKVNIWSLMTAE
NLWSVEIKQPEVMVVRNYTPLPLKFNPASKEIEILKDGDNADGKLSFYIKKYENGEVA
RWLHHLPKGHIIEIRGPFIDYEFPHLPNELKRSRDCLYMDNRNERGNNVRENSQFIYQ
PYDIMMFTAGTGIVTALQLLLTESPFRGTIKLFHTDKNIKQLGPLYPILLRLQASNRV
QLKIFETDRQTKQDVLKSIQKSITKPYPYKGLLPFSNVNNKNIMPVLALVCGPESYIS
SISGRKYDLNQGPVGGLLSKEGWNSDNVYKLS"
gene complement(<402761..>405388)
/gene="HIR2"
/locus_tag="YOR038C"
/gene_synonym="SPT1"
/db_xref="GeneID:854203"
mRNA complement(<402761..>405388)
/gene="HIR2"
/locus_tag="YOR038C"
/gene_synonym="SPT1"
/product="Hir2p"
/transcript_id="NM_001183457.1"
/db_xref="GeneID:854203"
CDS complement(402761..405388)
/gene="HIR2"
/locus_tag="YOR038C"
/gene_synonym="SPT1"
/experiment="EXISTENCE:direct assay:GO:0000122 negative
regulation of transcription by RNA polymerase II
[PMID:9001207]"
/experiment="EXISTENCE:direct assay:GO:0000417 HIR complex
[PMID:16264190|PMID:16303565]"
/experiment="EXISTENCE:direct assay:GO:0003677 DNA binding
[PMID:16264190]"
/experiment="EXISTENCE:direct assay:GO:0003714
transcription corepressor activity [PMID:9001207]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476|PMID:8417331]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0006325 chromatin
organization [PMID:16264190|PMID:16303565]"
/experiment="EXISTENCE:genetic interaction:GO:0016480
negative regulation of transcription by RNA polymerase III
[PMID:23962978]"
/experiment="EXISTENCE:genetic interaction:GO:0140673
transcription elongation-coupled chromatin remodeling
[PMID:16449659]"
/experiment="EXISTENCE:mutant phenotype:GO:0016480
negative regulation of transcription by RNA polymerase III
[PMID:23962978]"
/experiment="EXISTENCE:mutant phenotype:GO:0031491
nucleosome binding [PMID:16264190]"
/experiment="EXISTENCE:physical interaction:GO:0000417 HIR
complex [PMID:16264190]"
/note="Subunit of HIR nucleosome assembly complex;
involved in regulation of histone gene transcription;
recruits Swi-Snf complexes to histone gene promoters;
promotes heterochromatic gene silencing with Asf1p;
relocalizes to the cytosol in response to hypoxia"
/codon_start=1
/product="Hir2p"
/protein_id="NP_014681.1"
/db_xref="GeneID:854203"
/db_xref="SGD:S000005564"
/translation="MRLLKYPLDIHNEQVNALAALGPYIILAGSGGHVMAWRQQQLVD
TAFDRVMIKDLKPEVSFQVDQDTTGDIFFITGDLETLYIGSEHRLWGYSGWLCRDTNN
INSVEKMNSKLLFECKSPSTITDVKYDINLGILFVLLSNENKILLFRHKTFDKLSEIT
IDKASKPITGIIDPTGQTFTVMTSDRSILVYQINKTGTHKLINKLTQHVQMYPLHYRI
SMSPQADILPVINSVKGVPNNATSCTALLDRNNNYKVTKTLVTPSSNGCRVLVYSPAF
YEKPNLKKGTSTRYNLIATSGSTDGTILVWNTKRMKPLFNALQVSSTAINDMSWSQDG
FTLFAISNDATLYTFAFQEKDLGVALPQTEIKSLQEVNKKLPKLEEPLAEQIPKSFPE
NIKLEESASAAPIPNDIGRSAVGKKPTKKKTANNQTNGIKTIQSTSMEFNTPSYTVPR
DLKRKPKEATPSNIAPGSKKQKKELQPIDFLDTGLLLPNTSFSRIRLATPKIRSTFKY
SPINNPNLILDVKNGSGNEQRPTIVKLTSKVLDQDQVLFQDFIPKLITICTAGDTFWS
FCSEDGSIYIYSDSGRKLMAPLVLGVSISFLEACGTYLLCLTSIGELYCWNIEQKKLA
FPTNTIYPLLNPSLRYSDDILTRAENITLCSITKKGVPLVTLSNGDGYLFDKNMETWL
LVSDGWWAYGSQYWDTTNTTGLSSSKANTDSFNGSESNINEIVSDIKNDNQSIINFLE
CKTNDELNRKGRIKNLQRFARTILMKEGFENMEEIVTLSHLENKILISIRLEEPEEFS
KLMMVYCIRLSELGYMDRLNDVFQWLYDDLPISGTGSAFADKDFKRNLLKKILIACGD
IRQVQRVTTRYAKEMNIIS"
gene <405768..>406544
/gene="CKB2"
/locus_tag="YOR039W"
/db_xref="GeneID:854204"
mRNA <405768..>406544
/gene="CKB2"
/locus_tag="YOR039W"
/product="casein kinase 2 regulatory subunit CKB2"
/transcript_id="NM_001183458.1"
/db_xref="GeneID:854204"
CDS 405768..406544
/gene="CKB2"
/locus_tag="YOR039W"
/experiment="EXISTENCE:direct assay:GO:0005956 protein
kinase CK2 complex [PMID:8135547]"
/experiment="EXISTENCE:direct assay:GO:0006356 regulation
of transcription by RNA polymerase I [PMID:11551505]"
/experiment="EXISTENCE:direct assay:GO:0006359 regulation
of transcription by RNA polymerase III [PMID:11551505]"
/experiment="EXISTENCE:direct assay:GO:0006974 DNA damage
response [PMID:11551505]"
/experiment="EXISTENCE:direct assay:GO:0030291 protein
serine/threonine kinase inhibitor activity
[PMID:18265947]"
/experiment="EXISTENCE:direct assay:GO:0032545 CURI
complex [PMID:17452446]"
/experiment="EXISTENCE:direct assay:GO:0034456 UTP-C
complex [PMID:17515605]"
/experiment="EXISTENCE:mutant phenotype:GO:0019887 protein
kinase regulator activity [PMID:11827175]"
/note="Beta' regulatory subunit of casein kinase 2 (CK2);
a Ser/Thr protein kinase with roles in cell growth and
proliferation; CK2, comprised of CKA1, CKA2, CKB1 and
CKB2, has many substrates including transcription factors
and all RNA polymerase"
/codon_start=1
/product="casein kinase 2 regulatory subunit CKB2"
/protein_id="NP_014682.1"
/db_xref="GeneID:854204"
/db_xref="SGD:S000005565"
/translation="MGSRSENVGTVTREGSRVEQDDVLMDDDSDSSEYVDMWIDLFLG
RKGHEYFCDVDPEYITDRFNLMNLQKTVSKFSYVVQYIVDDLDDSILENMTHARLEQL
ESDSRKLYGLIHARYIITIKGLQKMYAKYKEADFGRCPRVYCNLQQLLPVGLHDIPGI
DCVKLYCPSCEDLYIPKSSRHSSIDGAYFGTSFPGMFLQAFPDMVPKHPTKRYVPKIF
GFELHKQAQLTRWQELQRLKLVEKLESKDVDLTKSGGFKT"
gene <407064..>407921
/gene="GLO4"
/locus_tag="YOR040W"
/db_xref="GeneID:854205"
mRNA <407064..>407921
/gene="GLO4"
/locus_tag="YOR040W"
/product="hydroxyacylglutathione hydrolase GLO4"
/transcript_id="NM_001183459.1"
/db_xref="GeneID:854205"
CDS 407064..407921
/gene="GLO4"
/locus_tag="YOR040W"
/EC_number="3.1.2.6"
/experiment="EXISTENCE:direct assay:GO:0004416
hydroxyacylglutathione hydrolase activity [PMID:9261170]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:9261170]"
/experiment="EXISTENCE:direct assay:GO:0019243
methylglyoxal catabolic process to D-lactate via
S-lactoyl-glutathione [PMID:9261170]"
/experiment="EXISTENCE:genetic interaction:GO:0004416
hydroxyacylglutathione hydrolase activity [PMID:9261170]"
/experiment="EXISTENCE:genetic interaction:GO:0019243
methylglyoxal catabolic process to D-lactate via
S-lactoyl-glutathione [PMID:9261170]"
/experiment="EXISTENCE:mutant phenotype:GO:0004416
hydroxyacylglutathione hydrolase activity [PMID:9261170]"
/experiment="EXISTENCE:mutant phenotype:GO:0019243
methylglyoxal catabolic process to D-lactate via
S-lactoyl-glutathione [PMID:9261170]"
/note="Mitochondrial glyoxalase II; catalyzes the
hydrolysis of S-D-lactoylglutathione into glutathione and
D-lactate; GLO4 has a paralog, GLO2, that arose from the
whole genome duplication"
/codon_start=1
/product="hydroxyacylglutathione hydrolase GLO4"
/protein_id="NP_014683.1"
/db_xref="GeneID:854205"
/db_xref="SGD:S000005566"
/translation="MKFLLQQIRNMHVKPIKMRWLTGGVNYSYLLSTEDRRNSWLIDP
AEPLEVSPKLSAEEKKSIDAIVNTHHHYDHSGGNLALYSILCQENSGHDIKIIGGSKS
SPGVTEVPDNLQQYHLGNLRVTCIRTPCHTKDSICYYIKDLETGEQCIFTGDTLFIAG
CGRFFEGTGRDMDMALNQIMLRAVGETNWNKVKIYPGHEYTKGNVSFIRAKIYSDIGQ
NKEFDALEQYCKSNECTTGHFTLRDELGYNPFMRLDDRAVRLAVGDTAGTYPRSVVMQ
ELRKLKNAM"
gene complement(407948..408134)
/gene="SNR9"
/locus_tag="YNCO0014C"
/db_xref="GeneID:9164974"
ncRNA complement(407948..408134)
/ncRNA_class="snoRNA"
/gene="SNR9"
/locus_tag="YNCO0014C"
/product="SNR9"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:3327689]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:3327689]"
/experiment="EXISTENCE:physical interaction:GO:0003723 RNA
binding [PMID:3327689]"
/experiment="EXISTENCE:physical interaction:GO:0031429 box
H/ACA snoRNP complex [PMID:15923376]"
/note="H/ACA box small nucleolar RNA (snoRNA); guides
pseudouridylation of large subunit (LSU) rRNA at position
U2340 and U2345"
/transcript_id="NR_132258.1"
/db_xref="GeneID:9164974"
/db_xref="SGD:S000007292"
gene <408425..>409660
/gene="CUE5"
/locus_tag="YOR042W"
/db_xref="GeneID:854206"
mRNA <408425..>409660
/gene="CUE5"
/locus_tag="YOR042W"
/product="ubiquitin-binding protein CUE5"
/transcript_id="NM_001183461.1"
/db_xref="GeneID:854206"
CDS 408425..409660
/gene="CUE5"
/locus_tag="YOR042W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0036435 K48-linked
polyubiquitin modification-dependent protein binding
[PMID:28525741]"
/experiment="EXISTENCE:direct assay:GO:0043130 ubiquitin
binding [PMID:12628920]"
/experiment="EXISTENCE:direct assay:GO:0070530 K63-linked
polyubiquitin modification-dependent protein binding
[PMID:28525741]"
/experiment="EXISTENCE:mutant phenotype:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:25042851]"
/experiment="EXISTENCE:mutant phenotype:GO:0006995
cellular response to nitrogen starvation [PMID:25042851]"
/experiment="EXISTENCE:mutant phenotype:GO:0030674
protein-macromolecule adaptor activity [PMID:25042851]"
/note="Selective autophagy receptor involved in aggrephagy
and proteaphagy; ubiquitin (Ub)-binding protein that
functions as a Ub-Atg8p adaptor in Ub-dependent autophagy;
serves as a proteaphagy receptor for inactivate 26S
proteasomes; contains a CUE domain that binds K48-and
K63-linked Ub chains and monoubiquitin, facilitating
intramolecular monoubiquitination; human TOLLIP is a
functional CUE-domain homolog, that complements a null
mutant, rescuing the hypersensitivity of the null mutant
to Htt-96Q"
/codon_start=1
/product="ubiquitin-binding protein CUE5"
/protein_id="NP_014685.1"
/db_xref="GeneID:854206"
/db_xref="SGD:S000005568"
/translation="MEEKEGIKDSSLLEKSNVPESINEDISKTTDVDLNSDGKKDNDT
SAKDGTPKVEEKVNKSSGIDEDEVVTPAEDAKEEEEEHPPLPARRKSEEEPSKENPIL
QELKDAFPNLEEKYIKAVIIASQGVLSPAFNALLFLSDPESGKDIELPTQPVRKNPEA
PARRRQTQLEQDELLARQLDEQFNSSHSRRRNRDRATRSMHEQRRRRHNPNEREQHHE
DSEEEDSWSQFVEKDLPELTDRAGRSLQDTANKVSNWISDAYRRNFASGNEQNDNQHG
HQDQQEWEPEIVDLSQGGKNSRPQQPERRRFNSFGVQVGDDSLESHGITLHNEDGFED
DEDVPPQLPTRTKSGESTGKVVAETTYIDTPDTETKKKWQPLPPEPLDTTPTKVNAVS
RNKKNPDEDEFLINSDDEM"
gene complement(409765..409864)
/gene="SNR62"
/locus_tag="YNCO0015C"
/db_xref="GeneID:9164978"
ncRNA complement(409765..409864)
/ncRNA_class="snoRNA"
/gene="SNR62"
/locus_tag="YNCO0015C"
/product="SNR62"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:10024243]"
/experiment="EXISTENCE:curator inference:GO:0031428 box
C/D methylation guide snoRNP complex [PMID:10024243]"
/experiment="EXISTENCE:mutant phenotype:GO:0030562 rRNA
2'-O-ribose methylation guide activity [PMID:10024243]"
/experiment="EXISTENCE:mutant phenotype:GO:0031167 rRNA
methylation [PMID:10024243]"
/note="C/D box small nucleolar RNA (snoRNA); guides
2'-O-methylation of large subunit (LSU) rRNA at position
U1888"
/transcript_id="NR_132259.1"
/db_xref="GeneID:9164978"
/db_xref="SGD:S000006453"
gene <410870..>412330
/gene="WHI2"
/locus_tag="YOR043W"
/db_xref="GeneID:854208"
mRNA <410870..>412330
/gene="WHI2"
/locus_tag="YOR043W"
/product="Whi2p"
/transcript_id="NM_001183462.1"
/db_xref="GeneID:854208"
CDS 410870..412330
/gene="WHI2"
/locus_tag="YOR043W"
/experiment="EXISTENCE:direct assay:GO:0019903 protein
phosphatase binding [PMID:12090248]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0034198
cellular response to amino acid starvation
[PMID:30142151]"
/experiment="EXISTENCE:genetic interaction:GO:1904262
negative regulation of TORC1 signaling [PMID:30142151]"
/experiment="EXISTENCE:mutant phenotype:GO:0000423
mitophagy [PMID:21429936]"
/experiment="EXISTENCE:mutant phenotype:GO:0006897
endocytosis [PMID:15020461]"
/experiment="EXISTENCE:mutant phenotype:GO:0007015 actin
filament organization [PMID:15020461]"
/experiment="EXISTENCE:mutant phenotype:GO:0034198
cellular response to amino acid starvation
[PMID:30142151]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress [PMID:12090248]"
/experiment="EXISTENCE:mutant phenotype:GO:0034605
cellular response to heat [PMID:12090248]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:12090248]"
/experiment="EXISTENCE:mutant phenotype:GO:0071474
cellular hyperosmotic response [PMID:12090248]"
/experiment="EXISTENCE:mutant phenotype:GO:1903452
positive regulation of G1 to G0 transition [PMID:7001255]"
/experiment="EXISTENCE:mutant phenotype:GO:1904262
negative regulation of TORC1 signaling [PMID:30142151]"
/note="Negative regulator of TORC1 in response to limiting
leucine; suppresses TORC1 activity with binding partners
Psr1p/Psr2p, acting in parallel with SEACIT; regulates
cell cycle arrest in stationary phase; inhibits
Ras-cAMP-PKA regulation of apoptosis during nutrient
depletion; required with Psr1p for activation of the
general stress response; role in rapamycin-induced
mitophagy; localizes to the cell periphery; human tumor
suppressor and Whi2-like protein KCTD11 functionally
complements the null"
/codon_start=1
/product="Whi2p"
/protein_id="NP_014686.1"
/db_xref="GeneID:854208"
/db_xref="SGD:S000005569"
/translation="MDDIITQVSPDNAESAPILQEQQQQQNSQYEGNEEDYGDSLIHL
NIQENHYFITRDQLMSLPESLLLCLFPSGVFLDRCGQVITNLTRDDEVYIVNFPPDCF
EYIMEIYTKAHDDLYNHPVEKFFDRPSSSFVSNAKGFFGLSSNNSISSNNEQDILHQK
PAIIVLREDLDYYCVPQEEFQFDSTNEENNEDLLRHFMAQVKMAAGSYLTSKTSIFQG
LYSSNRLKQQQQQQKIEKGSNSSSNTKSTSKKLGPAEQHLMDMLCSSGFTKETCWGNR
TQETGKTVISSLSLCRLANETTEGFRQKFNEAKAKWEAEHKPSQDNFITPMQSNISIN
SLSASKSNSTISTARNLTSGSTAPATARDKRKSRLSKLADNVRSHSSSRHSSQTRSKP
PELPKLYDLVPKPNINAKLLLFWRKPARKCWWGEEDIELEVEVFGSWKDESKKIIELI
LPTNVDPEAELHKIIVPVRLHIRRVWTLELSVIGVQ"
gene <413007..>413480
/gene="IRC23"
/locus_tag="YOR044W"
/db_xref="GeneID:854209"
mRNA <413007..>413480
/gene="IRC23"
/locus_tag="YOR044W"
/product="Irc23p"
/transcript_id="NM_001183463.1"
/db_xref="GeneID:854209"
CDS 413007..413480
/gene="IRC23"
/locus_tag="YOR044W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095]"
/note="hypothetical protein involved in multidrug
resistance; green fluorescent protein (GFP)-fusion
localizes to the ER; null mutant displays increased levels
of spontaneous Rad52p foci; IRC23 has a paralog, BSC2,
that arose from the whole genome duplication"
/codon_start=1
/product="Irc23p"
/protein_id="NP_014687.1"
/db_xref="GeneID:854209"
/db_xref="SGD:S000005570"
/translation="MIEALEIVLLLVIQSLQYICRTCIAFLLIPFLGLYAFDLFLYVY
RMILYLSQMFNYKRKLGRSKTNNRPHSPRLHKIYSSGDCMDTLIGQVRDLRVFLLSTI
HSHSKRFFSTRFQTKSGINSAIDANDVETTSDVSSFTNLHLTRSSEEGYYIAGSI"
gene <413852..>414037
/gene="TOM6"
/locus_tag="YOR045W"
/gene_synonym="ISP6; MOM8B"
/db_xref="GeneID:854210"
mRNA <413852..>414037
/gene="TOM6"
/locus_tag="YOR045W"
/gene_synonym="ISP6; MOM8B"
/product="Tom6p"
/transcript_id="NM_001183464.1"
/db_xref="GeneID:854210"
CDS 413852..414037
/gene="TOM6"
/locus_tag="YOR045W"
/gene_synonym="ISP6; MOM8B"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0008320 protein
transmembrane transporter activity [PMID:7565772]"
/experiment="EXISTENCE:direct assay:GO:0070096
mitochondrial outer membrane translocase complex assembly
[PMID:20668160]"
/experiment="EXISTENCE:mutant phenotype:GO:0005742
mitochondrial outer membrane translocase complex
[PMID:7565772|PMID:9774667]"
/experiment="EXISTENCE:mutant phenotype:GO:0030150 protein
import into mitochondrial matrix [PMID:9774667]"
/note="Component of the TOM (translocase of outer
membrane) complex; responsible for recognition and initial
import steps for all mitochondrially directed proteins;
promotes assembly and stability of the TOM complex"
/codon_start=1
/product="Tom6p"
/protein_id="NP_014688.1"
/db_xref="GeneID:854210"
/db_xref="SGD:S000005571"
/translation="MDGMFAMPGAAAGAASPQQPKSRFQAFKESPLYTIALNGAFFVA
GVAFIQSPLMDMLAPQL"
gene complement(<414459..>415907)
/gene="DBP5"
/locus_tag="YOR046C"
/gene_synonym="RAT8"
/db_xref="GeneID:854211"
mRNA complement(<414459..>415907)
/gene="DBP5"
/locus_tag="YOR046C"
/gene_synonym="RAT8"
/product="ATP-dependent RNA helicase DBP5"
/transcript_id="NM_001183465.1"
/db_xref="GeneID:854211"
CDS complement(414459..415907)
/gene="DBP5"
/locus_tag="YOR046C"
/gene_synonym="RAT8"
/EC_number="3.6.4.13"
/experiment="EXISTENCE:direct assay:GO:0000822 inositol
hexakisphosphate binding [PMID:16783363]"
/experiment="EXISTENCE:direct assay:GO:0003724 RNA
helicase activity [PMID:9564047]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:15280434]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:9564048|PMID:15280434|PMID:10610322]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:19198597]"
/experiment="EXISTENCE:direct assay:GO:0006409 tRNA export
from nucleus [PMID:31453808]"
/experiment="EXISTENCE:direct assay:GO:0008186
ATP-dependent activity, acting on RNA [PMID:19805289]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:27251550]"
/experiment="EXISTENCE:direct assay:GO:0044614 nuclear
pore cytoplasmic filaments [PMID:10610322]"
/experiment="EXISTENCE:genetic interaction:GO:0006415
translational termination [PMID:17272721]"
/experiment="EXISTENCE:mutant phenotype:GO:0006406 mRNA
export from nucleus [PMID:9564048]"
/experiment="EXISTENCE:mutant phenotype:GO:0016973
poly(A)+ mRNA export from nucleus [PMID:27385342]"
/experiment="EXISTENCE:physical interaction:GO:0006415
translational termination [PMID:17272721]"
/note="Cytoplasmic ATP-dependent RNA helicase of the
DEAD-box family; involved in mRNA export from the nucleus,
remodeling messenger ribonucleoprotein particles (mRNPs),
with ATPase activity stimulated by Gle1p, IP6 and Nup159p;
involved in translation termination along with Sup45p
(eRF1); role in the cellular response to heat stress"
/codon_start=1
/product="ATP-dependent RNA helicase DBP5"
/protein_id="NP_014689.1"
/db_xref="GeneID:854211"
/db_xref="SGD:S000005572"
/translation="MSDTKRDPADLLASLKIDNEKEDTSEVSTKETVKSQPEKTADSI
KPAEKLVPKVEEKKTKQEDSNLISSEYEVKVKLADIQADPNSPLYSAKSFDELGLAPE
LLKGIYAMKFQKPSKIQERALPLLLHNPPRNMIAQSQSGTGKTAAFSLTMLTRVNPED
ASPQAICLAPSRELARQTLEVVQEMGKFTKITSQLIVPDSFEKNKQINAQVIVGTPGT
VLDLMRRKLMQLQKIKIFVLDEADNMLDQQGLGDQCIRVKRFLPKDTQLVLFSATFAD
AVRQYAKKIVPNANTLELQTNEVNVDAIKQLYMDCKNEADKFDVLTELYGLMTIGSSI
IFVATKKTANVLYGKLKSEGHEVSILHGDLQTQERDRLIDDFREGRSKVLITTNVLAR
GIDIPTVSMVVNYDLPTLANGQADPATYIHRIGRTGRFGRKGVAISFVHDKNSFNILS
AIQKYFGDIEMTRVPTDDWDEVEKIVKKVLKD"
gene complement(<416347..>417681)
/gene="STD1"
/locus_tag="YOR047C"
/gene_synonym="MSN3; SFS3"
/db_xref="GeneID:854212"
mRNA complement(<416347..>417681)
/gene="STD1"
/locus_tag="YOR047C"
/gene_synonym="MSN3; SFS3"
/product="Std1p"
/transcript_id="NM_001183466.1"
/db_xref="GeneID:854212"
CDS complement(416347..417681)
/gene="STD1"
/locus_tag="YOR047C"
/gene_synonym="MSN3; SFS3"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10373505]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:10373505]"
/experiment="EXISTENCE:direct assay:GO:0030295 protein
kinase activator activity [PMID:12618390]"
/experiment="EXISTENCE:genetic interaction:GO:0071590
nicotinamide riboside biosynthetic process
[PMID:19846558]"
/experiment="EXISTENCE:genetic interaction:GO:0071592
nicotinic acid riboside biosynthetic process
[PMID:19846558]"
/experiment="EXISTENCE:mutant phenotype:GO:0006006 glucose
metabolic process [PMID:10373505]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:7667094]"
/experiment="EXISTENCE:mutant phenotype:GO:0007165 signal
transduction [PMID:10373505]"
/experiment="EXISTENCE:mutant phenotype:GO:0009651
response to salt stress [PMID:9725828]"
/experiment="EXISTENCE:mutant phenotype:GO:0071590
nicotinamide riboside biosynthetic process
[PMID:19846558]"
/experiment="EXISTENCE:mutant phenotype:GO:0071592
nicotinic acid riboside biosynthetic process
[PMID:19846558]"
/note="Protein involved in control of glucose-regulated
gene expression; interacts with kinase Snf1p, glucose
sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of
transcription factor Rgt1p; interactions with Pma1p appear
to propagate [GAR+]; STD1 has a paralog, MTH1, that arose
from the whole genome duplication"
/codon_start=1
/product="Std1p"
/protein_id="NP_014690.1"
/db_xref="GeneID:854212"
/db_xref="SGD:S000005573"
/translation="MFVSPPPATARNQVLGKRKSKRHDENPKNVQPNADTEMTNSVPS
IGFNSNLPHNNQEINTPNHYNLSSNSGNVRSNNNFVTTPPEYADRARIEIIKRLLPTA
GTKPMEVNSNTAENANIQHINTPDSQSFVSDHSSSYESSIFSQPSTALTDITTGSSLI
DTKTPKFVTEVTLEDALPKTFYDMYSPEVLMSDPANILYNGRPKFTKRELLDWDLNDI
RSLLIVEQLRPEWGSQLPTVVTSGINLPQFRLQLLPLSSSDEFIIATLVNSDLYIEAN
LDRNFKLTSAKYTVASARKRHEEMTGSKEPIMRLSKPEWRNIIENYLLNVAVEAQCRY
DFKQKRSEYKRWKLLNSNLKRPDMPPPSLIPHGFKIHDCTNSGSLLKKALMKNLQLKN
YKNDAKTLGAGTQKNVVNKVSLTSEERAAIWFQCQTQVYQRLGLDWKPDGMS"
gene complement(<418630..>421650)
/gene="RAT1"
/locus_tag="YOR048C"
/gene_synonym="HKE1; TAP1; XRN2"
/db_xref="GeneID:854213"
mRNA complement(<418630..>421650)
/gene="RAT1"
/locus_tag="YOR048C"
/gene_synonym="HKE1; TAP1; XRN2"
/product="ssRNA exonuclease RAT1"
/transcript_id="NM_001183467.1"
/db_xref="GeneID:854213"
CDS complement(418630..421650)
/gene="RAT1"
/locus_tag="YOR048C"
/gene_synonym="HKE1; TAP1; XRN2"
/experiment="EXISTENCE:direct assay:GO:0000448 cleavage in
ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:26638174]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0004534 5'-3' RNA
exonuclease activity [PMID:8417335]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922|PMID:9315672]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0110155 NAD-cap
decapping [PMID:35173156]"
/experiment="EXISTENCE:genetic interaction:GO:0006364 rRNA
processing [PMID:9488433]"
/experiment="EXISTENCE:genetic interaction:GO:0030847
termination of RNA polymerase II transcription,
exosome-dependent [PMID:19818713]"
/experiment="EXISTENCE:genetic interaction:GO:0034244
negative regulation of transcription elongation by RNA
polymerase II [PMID:24501251]"
/experiment="EXISTENCE:genetic interaction:GO:0043144
sno(s)RNA processing [PMID:9488433]"
/experiment="EXISTENCE:genetic interaction:GO:0071028
nuclear mRNA surveillance [PMID:11030620]"
/experiment="EXISTENCE:mutant phenotype:GO:0000398 mRNA
splicing, via spliceosome [PMID:33978753]"
/experiment="EXISTENCE:mutant phenotype:GO:0006364 rRNA
processing [PMID:9488433|PMID:11142370]"
/experiment="EXISTENCE:mutant phenotype:GO:0030846
termination of RNA polymerase II transcription,
poly(A)-coupled [PMID:15565157]"
/experiment="EXISTENCE:mutant phenotype:GO:0030847
termination of RNA polymerase II transcription,
exosome-dependent [PMID:19818713]"
/experiment="EXISTENCE:mutant phenotype:GO:0034244
negative regulation of transcription elongation by RNA
polymerase II [PMID:24501251]"
/experiment="EXISTENCE:mutant phenotype:GO:0043144
sno(s)RNA processing [PMID:9488433]"
/experiment="EXISTENCE:mutant phenotype:GO:0071028 nuclear
mRNA surveillance [PMID:11030620|PMID:27124216]"
/experiment="EXISTENCE:mutant phenotype:GO:0071035 nuclear
polyadenylation-dependent rRNA catabolic process
[PMID:16131592]"
/note="Nuclear 5' to 3' single-stranded RNA exonuclease;
involved in RNA metabolism, including rRNA and snoRNA
processing, as well as poly (A+) dependent and independent
mRNA transcription termination; required for
cotranscriptional pre-rRNA cleavage; displaces Cdk1p from
elongating transcripts, especially as RNAPII reaches the
poly(A) site, negatively regulates phosphorylation of the
CTD of RNAPII, and inhibits RNAPII transcriptional
elongation"
/codon_start=1
/product="ssRNA exonuclease RAT1"
/protein_id="NP_014691.1"
/db_xref="GeneID:854213"
/db_xref="SGD:S000005574"
/translation="MGVPSFFRWLSRKYPKIISPVLEEQPQIVDGVILPLDYSASNPN
GELDNLYLDMNGIVHPCSHPENKPPPETEDEMLLAVFEYTNRVLNMARPRKVLVMAVD
GVAPRAKMNQQRARRFRSARDAQIENEAREEIMRQREEVGEIIDDAVRNKKTWDSNAI
TPGTPFMDKLAAALRYWTAFKLATDPGWKNLQVIISDATVPGEGEHKIMNFIRSQRAD
PEYNPNTTHCIYGLDADLIFLGLATHEPHFKILREDVFAQDNRKRNNLKDTINMTEEE
KQFLQKQNSEQPFLWLHINVLREYLSAELWVPGLPFTFDLERAIDDWVFMCFFCGNDF
LPHLPCLDVRENSIDILLDIWKVVLPKLKTYMTCDGVLNLPSVETLLQHLGSREGDIF
KTRHIQEARKKEAFERRKAQKNMSKGQDRHPTVATEQLQMYDTQGNLAKGSWNLTTSD
MVRLKKELMLANEGNEEAIAKVKQQSDKNNELMKDISKEEIDDAVSKANKTNFNLAEV
MKQKIINKKHRLEKDNEEEEIAKDSKKVKTEKAESECDLDAEIKDEIVADVNDRENSE
TTEVSRDSPVHSTVNVSEGPKNGVFDTDEFVKLFEPGYHERYYTAKFHVTPQDIEQLR
KDMVKCYIEGVAWVLMYYYQGCASWNWFYPYHYAPLATDFHGFSHLEIKFEEGTPFLP
YEQLMSVLPAASGHALPKIFRSLMSEPDSEIIDFYPEEFPIDMNGKKMSWQGIALLPF
IDQDRLLTAVRAQYPLLSDAERARNIRGEPVLLISNKNANYERFSKKLYSKENNNNNV
VVKFQHFKSGLSGIVSKDVEGFELNGKIVCPIQGGSLPNLSTTLILKMSYRLIPLPSR
NKSIILNGFIPSEPVLTAYDLDSIMYKYNNQNYSRRWNFGNDLKQNIVPVGPKGITQY
KPRTGGYRAFFYFAELSRNNVQPAHNYGRNSYNSQPGFNNSRYDGGNNNYRQNSNYRN
NNYSGNRNSGQYSGNSYSRNNKQSRYDNSRANRR"
gene complement(<422668..>423732)
/gene="RSB1"
/locus_tag="YOR049C"
/db_xref="GeneID:854214"
mRNA complement(<422668..>423732)
/gene="RSB1"
/locus_tag="YOR049C"
/product="phospholipid-translocating ATPase RSB1"
/transcript_id="NM_001183468.1"
/db_xref="GeneID:854214"
CDS complement(422668..423732)
/gene="RSB1"
/locus_tag="YOR049C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005324 long-chain
fatty acid transmembrane transporter activity
[PMID:28175317]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:12034738]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16407254|PMID:12034738]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:12034738]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:24146988]"
/experiment="EXISTENCE:direct assay:GO:1905329 sphingoid
long-chain base transport [PMID:12034738]"
/experiment="EXISTENCE:mutant phenotype:GO:0045332
phospholipid translocation [PMID:15342785]"
/experiment="EXISTENCE:mutant phenotype:GO:1905329
sphingoid long-chain base transport [PMID:12034738]"
/note="Sphingoid long-chain base (LCB) efflux transporter;
integral membrane transporter that localizes to the plasma
membrane and may transport long chain bases (LCBs) from
the cytoplasmic side toward the extracytoplasmic side of
the membrane; role in glycerophospholipid translocation;
suppressor of the sphingoid LCB sensitivity of an
LCB-lyase mutation"
/codon_start=1
/product="phospholipid-translocating ATPase RSB1"
/protein_id="NP_014692.1"
/db_xref="GeneID:854214"
/db_xref="SGD:S000005575"
/translation="MVPNLRFNITMIVIWGILLTIHVVQLLMRQYWFSIAFICTGILE
VLGFIGRTWSHSNVADMDAFLLNMICLTIAPVFTMGGIYYQLAKLIEVYGHRFSLLPS
PMAYSFIFICSDIVSLVVQAVGGGLCGVAVTDGTSTTTGNHVFIAGLAIQVASMAIFL
MLWFHFLFRIYISVRWEHINSRPISLSLLKISQTEVDYLYREKFHFLRLEPKRWVFHY
FNLAITVAVLTIFTRCCYRLAELVVGWDGYLITHEWYFIILDALMMAIATVTLTIFHP
GFAFKGRSTSIPITPGHVDPETLPHTDDVEDILDTSDSKQFDIEKEEFQASMKYPIST
FKQFMSKIANLFSSKKKAKL"
gene complement(<424846..>426084)
/gene="ETT1"
/locus_tag="YOR051C"
/gene_synonym="NRO1; YOR29-02"
/db_xref="GeneID:854216"
mRNA complement(<424846..>426084)
/gene="ETT1"
/locus_tag="YOR051C"
/gene_synonym="NRO1; YOR29-02"
/product="Ett1p"
/transcript_id="NM_001183470.1"
/db_xref="GeneID:854216"
CDS complement(424846..426084)
/gene="ETT1"
/locus_tag="YOR051C"
/gene_synonym="NRO1; YOR29-02"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:26359986|PMID:14562095|PMID:10684247]"
/experiment="EXISTENCE:genetic interaction:GO:0006415
translational termination [PMID:20630870]"
/experiment="EXISTENCE:mutant phenotype:GO:0006415
translational termination [PMID:20630870]"
/note="Nuclear protein that inhibits replication of Brome
mosaic virus; S. cerevisiae is a model system for studying
replication of positive-strand RNA viruses in their
natural hosts; deletion increases stop codon readthrough"
/codon_start=1
/product="Ett1p"
/protein_id="NP_014694.1"
/db_xref="GeneID:854216"
/db_xref="SGD:S000005577"
/translation="MAKRPLGLGKQSREKKRKVESVEKKSDEPSRESTPVRSQMSVEL
DDDADLDDELAQLKGLWSKYFHSDRDDEYVLNGIVHECDRLLRLSEEDKEIKKTLNDI
FHGIYALALSELTIFKAGDEEATEEKRKKDVSSFFESAIERVELGLSHFPESQFLKLV
LAKIIFQRIPLEYISNLHLKSKDKKLDLVGQLEHGKKHFSIYENDTEFTFEILQMVND
LLDIVENFGREQSIQEGIDSDNEEEEELIDIELEPEHPVYPLQQSLEANYEWLRNHFD
KLLDNTNTDVKIYASIANTLGELYLKKAEEPSKVFLSLQYDDGGSEKVSDKEAKNAQE
TALKHTKKALEYLEKAKLEDDPDTWVQVAEAYIDLGNLLDNESAEQEEAYKTAEEILG
KANKASHGKFQDVLDNFLQG"
gene complement(<426772..>427224)
/gene="TMC1"
/locus_tag="YOR052C"
/gene_synonym="YOR29-03"
/db_xref="GeneID:854217"
mRNA complement(<426772..>427224)
/gene="TMC1"
/locus_tag="YOR052C"
/gene_synonym="YOR29-03"
/product="Tmc1p"
/transcript_id="NM_001183471.1"
/db_xref="GeneID:854217"
CDS complement(426772..427224)
/gene="TMC1"
/locus_tag="YOR052C"
/gene_synonym="YOR29-03"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0071218 cellular
response to misfolded protein [PMID:27226598]"
/experiment="EXISTENCE:direct assay:GO:0071243 cellular
response to arsenic-containing substance [PMID:27226598]"
/experiment="EXISTENCE:mutant phenotype:GO:0071243
cellular response to arsenic-containing substance
[PMID:24297164]"
/note="AN1-type zinc finger protein, effector of
proteotoxic stress response; stress-inducible
transcriptional target of Rpn4p; induced by nitrogen
limitation, weak acid, misfolded proteins; short-lived
protein, degraded by proteasome; may protect cells from
trivalent metalloid induced proteotoxicity; contains PACE
promoter element; ortholog of human AIRAP, which
stimulates proteasome activity in response to arsenic;
protein abundance increases under DNA replication stress"
/codon_start=1
/product="Tmc1p"
/protein_id="NP_014695.1"
/db_xref="GeneID:854217"
/db_xref="SGD:S000005578"
/translation="MSDINEIEIPSRKDEIRQVTPKDPMHEIEDKSTYHAKIKKSDSG
TVLGAIPLNSRSSSNSSVTSTGQSSRRVTKKTTKKKKKNACYFDTCSSAASKFIGDCN
FCKGHFCSKHRLMENHACNGLTSCKEQLHQRNADKLEAEQTKAPKIQI"
gene complement(<427833..>429857)
/gene="VHS3"
/locus_tag="YOR054C"
/gene_synonym="YOR29-05"
/db_xref="GeneID:854220"
mRNA complement(<427833..>429857)
/gene="VHS3"
/locus_tag="YOR054C"
/gene_synonym="YOR29-05"
/product="phosphopantothenoylcysteine decarboxylase
complex subunit VHS3"
/transcript_id="NM_001183473.1"
/db_xref="GeneID:854220"
CDS complement(427833..429857)
/gene="VHS3"
/locus_tag="YOR054C"
/gene_synonym="YOR29-05"
/experiment="EXISTENCE:direct assay:GO:0004633
phosphopantothenoylcysteine decarboxylase activity
[PMID:19915539]"
/experiment="EXISTENCE:direct assay:GO:0004864 protein
phosphatase inhibitor activity [PMID:15192104]"
/experiment="EXISTENCE:direct assay:GO:0071513
phosphopantothenoylcysteine decarboxylase complex
[PMID:19915539]"
/experiment="EXISTENCE:direct assay:GO:1990143
CoA-synthesizing protein complex [PMID:23789928]"
/experiment="EXISTENCE:genetic interaction:GO:0015937
coenzyme A biosynthetic process [PMID:19915539]"
/experiment="EXISTENCE:genetic interaction:GO:0030003
intracellular monoatomic cation homeostasis
[PMID:15192104]"
/experiment="EXISTENCE:mutant phenotype:GO:0004633
phosphopantothenoylcysteine decarboxylase activity
[PMID:19915539]"
/experiment="EXISTENCE:physical interaction:GO:0030003
intracellular monoatomic cation homeostasis
[PMID:15192104]"
/note="Negative regulatory subunit of protein phosphatase
1 Ppz1p; involved in coenzyme A biosynthesis; subunit of
the phosphopantothenoylcysteine decarboxylase (PPCDC;
Cab3p, Sis2p, Vhs3p) complex and the CoA-Synthesizing
Protein Complex (CoA-SPC: Cab2p, Cab3p, Cab4p, Cab5p,
Sis2p and Vhs3p)"
/codon_start=1
/product="phosphopantothenoylcysteine decarboxylase
complex subunit VHS3"
/protein_id="NP_014697.1"
/db_xref="GeneID:854220"
/db_xref="SGD:S000005580"
/translation="MTNKSSLKNNRKGVASNTLSGAEQANIGSSAMPDTNSTGPFSSV
SSLDTPVVRKSTSPTGSQTKSIMNASGTSGAVVSNTPEPGLKRIPTVTFSDPKLGSLR
SDVEQTPPNQVARQSSEKKATSVHIAAEGANQGRNLKDINTKVPKDGEASASSFSTPT
SILSNADMGNNISSLLAKKLSFTGGTDSILNSDNSSDSPRKEHPHFYVEDPLHTPSVR
SRSNSTSPRPSVVVNTFNPINIEREGSISKTGEPTLLESVLEEAMSPNAVSNPLKREN
IMTNMDPRLPQDDGKLHVLFGATGSLSVFKLKHMIRKLEEIYGRDKICIQVILTNSAT
KFFAMKYMRKNKKQHNSIDTSFNSTNSNAGNITGNKKKVASLEKFSIQKTSSNSAASQ
TNNKQEEEKQMASTTGFPSTLGGSRTYSNSSNVVSQHPQIELPAHIQFWTDQDEWDVW
RQRTDPVLHIELRRWADILVVAPLTANTLAKIALGLCDNLLTSVIRAWNPTFPIFLAP
SMGSGTFNSIMTKKHFRIIQEEMPWVTVFKPSEKVMGINGDIGLSGMMDANEIVGKIV
VKLGGYPDVSAGKEEEEDEDNDEEDDNKKNDTGGKDEDNDDDDDDDDDDDDDDDDDDD
DDDDDDDDDDDDDDDDDDDDDDDDDDEDDEDEDEDDEGKKKEDKGGLQRS"
gene complement(<430247..>431626)
/gene="NOB1"
/locus_tag="YOR056C"
/gene_synonym="YOR29-07"
/db_xref="GeneID:854221"
mRNA complement(<430247..>431626)
/gene="NOB1"
/locus_tag="YOR056C"
/gene_synonym="YOR29-07"
/product="rRNA-binding endoribonuclease"
/transcript_id="NM_001183475.1"
/db_xref="GeneID:854221"
CDS complement(430247..431626)
/gene="NOB1"
/locus_tag="YOR056C"
/gene_synonym="YOR29-07"
/experiment="EXISTENCE:direct assay:GO:0004521 RNA
endonuclease activity [PMID:19801658]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12502737|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0030490 maturation
of SSU-rRNA [PMID:19801658]"
/experiment="EXISTENCE:direct assay:GO:0030688
preribosome, small subunit precursor [PMID:12588997]"
/experiment="EXISTENCE:direct assay:GO:0070181 small
ribosomal subunit rRNA binding [PMID:19706509]"
/experiment="EXISTENCE:mutant phenotype:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:12588997|PMID:15388878]"
/experiment="EXISTENCE:mutant phenotype:GO:0043248
proteasome assembly [PMID:12502737]"
/experiment="EXISTENCE:physical interaction:GO:0043248
proteasome assembly [PMID:12502737]"
/note="Protein involved in proteasomal and 40S ribosomal
subunit biogenesis; required for cleavage of the 20S
pre-rRNA to generate the mature 18S rRNA; cleavage is
activated by Fun12p, a GTPase and translation initiation
factor; relocalizes from nucleus to nucleolus upon DNA
replication stress"
/codon_start=1
/product="rRNA-binding endoribonuclease"
/protein_id="NP_014699.1"
/db_xref="GeneID:854221"
/db_xref="SGD:S000005582"
/translation="MTENQTAHVRALILDATPLITQSYTHYQNYAQSFYTTPTVFQEI
KDAQARKNLEIWQSLGTLKLVHPSENSIAKVSTFAKLTGDYSVLSANDLHILALTYEL
EIKLNNGDWRLRKKPGDALDASKADVGTDGKQKLTEDNKKEEDSESVPKKKNKRRGGK
KQKAKREAREAREAENANLELESKAEEHVEEAGSKEQICNDENIKESSDLNEVFEDAD
DDGDWITPENLTEAIIKDSGEDTTGSLGVEASEEDRHVALNRPENQVALATGDFAVQN
VALQMNLNLMNFMSGLKIKRIRNYMLRCHACFKIFPLPKDGKPKHFCASCGGQGTLLR
CAVSVDSRTGNVTPHLKSNFQWNNRGNRYSVASPLSKNSQKRYGKKGHVHSKPQENVI
LREDQKEYEKVIKQEEWTRRHNEKILNNWIGGGSADNYISPFAITGLKQHNVRIGKGR
YVNSSKRRS"
gene <432186..>433373
/gene="SGT1"
/locus_tag="YOR057W"
/db_xref="GeneID:854222"
mRNA <432186..>433373
/gene="SGT1"
/locus_tag="YOR057W"
/product="co-chaperone SGT1"
/transcript_id="NM_001183476.1"
/db_xref="GeneID:854222"
CDS 432186..433373
/gene="SGT1"
/locus_tag="YOR057W"
/experiment="EXISTENCE:direct assay:GO:0000151 ubiquitin
ligase complex [PMID:10445024]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:34192536]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:34192536]"
/experiment="EXISTENCE:direct assay:GO:0006515 protein
quality control for misfolded or incompletely synthesized
proteins [PMID:34192536]"
/experiment="EXISTENCE:direct assay:GO:0030674
protein-macromolecule adaptor activity [PMID:16945921]"
/experiment="EXISTENCE:direct assay:GO:0051087
protein-folding chaperone binding
[PMID:34192536|PMID:16945921]"
/experiment="EXISTENCE:direct assay:GO:0051382 kinetochore
assembly [PMID:10445024]"
/experiment="EXISTENCE:direct assay:GO:0065003
protein-containing complex assembly [PMID:15090617]"
/note="Cochaperone protein; regulates activity of adenylyl
cyclase Cyr1p; involved in kinetochore complex assembly;
associates with the SCF (Skp1p/Cdc53p/F box protein)
ubiquitin ligase complex; acts as a linker between Skp1p
and HSP90 complexes; protein abundance increases in
response to DNA replication stress"
/codon_start=1
/product="co-chaperone SGT1"
/protein_id="NP_014700.1"
/db_xref="GeneID:854222"
/db_xref="SGD:S000005583"
/translation="MPVEKDLKTAYKALYDEKEPLKALHLYDEILKGSPTNLTALIFK
AACLEKLYFGFSDWHSDATMENAKELLDKALMTAEGRGDRSKIGLVNFRYFVHFFNIK
DYELAQSYFKKAKNLGYVDDTLPLWEDRLETKLNKKNKKQKDSTNKHTIKPVESIENR
GDNNSSHSPISPLKIETAPQESPKFKIDWYQSSTSVTISLFTVNLPESKEQVNIYISP
NDRRTLSISYQVPKSGSEFQYNAKLSHEVDPKAVSLKIFPKKLEITLSKIDSTQWKKL
EEDILTESSRLSDEGKNSDSATRLLSAETASKERLSYPSSSKKKIDWSKLDIDEEADE
EAGSADSFFQKLYAGADPDTKRAMMKSFIESNGTALSTDWEDVSKGTVKTSPPEGMEP
KHW"
gene complement(<433688..>436345)
/gene="ASE1"
/locus_tag="YOR058C"
/gene_synonym="YOR29-09"
/db_xref="GeneID:854223"
mRNA complement(<433688..>436345)
/gene="ASE1"
/locus_tag="YOR058C"
/gene_synonym="YOR29-09"
/product="Ase1p"
/transcript_id="NM_001183477.1"
/db_xref="GeneID:854223"
CDS complement(433688..436345)
/gene="ASE1"
/locus_tag="YOR058C"
/gene_synonym="YOR29-09"
/experiment="EXISTENCE:direct assay:GO:0001578 microtubule
bundle formation [PMID:12591913]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005874 microtubule
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005880 nuclear
microtubule [PMID:7559759]"
/experiment="EXISTENCE:direct assay:GO:0008017 microtubule
binding [PMID:12591913]"
/experiment="EXISTENCE:direct assay:GO:0072686 mitotic
spindle [PMID:7559759]"
/experiment="EXISTENCE:direct assay:GO:1990023 mitotic
spindle midzone [PMID:7559759]"
/experiment="EXISTENCE:genetic interaction:GO:0000022
mitotic spindle elongation [PMID:7559759]"
/experiment="EXISTENCE:genetic interaction:GO:0000073
initial mitotic spindle pole body separation
[PMID:16688214]"
/experiment="EXISTENCE:mutant phenotype:GO:0000022 mitotic
spindle elongation [PMID:7559759|PMID:12591913]"
/experiment="EXISTENCE:mutant phenotype:GO:0000920 septum
digestion after cytokinesis [PMID:16615892]"
/experiment="EXISTENCE:mutant phenotype:GO:0007052 mitotic
spindle organization [PMID:9036857]"
/experiment="EXISTENCE:mutant phenotype:GO:0051255 spindle
midzone assembly [PMID:17562791]"
/note="Mitotic spindle midzone-localized microtubule
bundling protein; microtubule-associated protein (MAP)
family member; required for anaphase spindle elongation
and stabilization; selectively increases the lifetime of
antiparallel microtubule overlaps; undergoes cell
cycle-regulated degradation by anaphase promoting complex;
relative distribution to microtubules decreases upon DNA
replication stress"
/codon_start=1
/product="Ase1p"
/protein_id="NP_116582.1"
/db_xref="GeneID:854223"
/db_xref="SGD:S000005584"
/translation="METATSSPLPIKSRRNSENSGSTTVIPHMNPSLATPLTVSTMVN
QSNSKEFMKLTPVRIRDFGSPLKNVSTNYHFLDSENGKGNTMDNMYRENFILISKDLE
KLLENLNVIYQNIGYSNTEIITKEKIIFTTISNSIKQFFEQADEELKRLSAENGIEQD
ILNNILERINDPSGIKTIPDLYIRNAILLQESKTVPQSPKKPLSLLSKKAALDTAKKF
VLGSFLPRLRDYLKSLITLKHLIQSVKENLPGLTEADNEAIAEFPELSTLTAYLLQIE
NGKGDIGLSMKFIIDNRKDILKGSAFKTINEESVKHMNEVIKIYEEEYERRFKSVLTK
KVSISSICEQLGTPLATLIGEDFEQDLRSYGEEENSTSEIPNFHPVDRERMSKIDITL
EKLQAIHKERADKKRLLMEQCQKLWTRLKISQEYIKTFMRNNSSLSTESLGRISKEVM
RLEAMKKKLIKKLISDSWDKIQELWRTLQYSEESRSKFIIVFEELRNSATTLQEDELL
LETCENELKRLEEKLTLYKPILKLISDFESLQEDQEFLERSSKDSSRLLSRNSHKILL
TEEKMRKRITRHFPRVINDLRIKLEEADGLFDQPFLFKGKPLSEAIDIQQQEIEAKYP
RCRVRMQRSKKGKCGANKENKVIKNTFKATESSIRVPIGLNLNDANITYKTPSKKTIQ
GLTKNDLSQENSLARHMQGTTKLSSPNRRATRLLAPTVISRNSKGNIERPTLNRNRSS
DLSSSPRINHTHGEHAVKPRQLFPIPLNKVDTKGSHIPQLTKEKALELLKRSTGTTGK
ENVRSPERKSSLEDYAQKLSSPYKEPEHSIYKLSMSPEGKFQLNIQQKDIESGFDDTS
MMEDENDKDFITWKNEQVSKLNGFSFTDI"
repeat_region complement(438175..438483)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007162"
gene 438643..438738
/locus_tag="YNCO0016W"
/db_xref="GeneID:854224"
tRNA join(438643..438679,438703..438738)
/locus_tag="YNCO0016W"
/product="tRNA-Lys"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE
analysis; thiolation of uridine at wobble position (34)
requires Ncs6p"
/db_xref="GeneID:854224"
/db_xref="SGD:S000006634"
gene complement(<438906..>440258)
/gene="LPL1"
/locus_tag="YOR059C"
/gene_synonym="YOR29-10"
/db_xref="GeneID:854225"
mRNA complement(<438906..>440258)
/gene="LPL1"
/locus_tag="YOR059C"
/gene_synonym="YOR29-10"
/product="putative hydrolase"
/transcript_id="NM_001183478.1"
/db_xref="GeneID:854225"
CDS complement(438906..440258)
/gene="LPL1"
/locus_tag="YOR059C"
/gene_synonym="YOR29-10"
/EC_number="3.1.1.4"
/experiment="EXISTENCE:direct assay:GO:0004622
phosphatidylcholine lysophospholipase A1 activity
[PMID:25014274]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005811 lipid
droplet [PMID:24868093|PMID:10515935|PMID:25014274]"
/experiment="EXISTENCE:mutant phenotype:GO:0004622
phosphatidylcholine lysophospholipase A1 activity
[PMID:25014274]"
/experiment="EXISTENCE:mutant phenotype:GO:0055088 lipid
homeostasis [PMID:25014274]"
/note="Phospholipase; contains lipase specific GXSXG
motif; maintains lipid droplet (LD) morphology; induced by
transcription factor Rpn4p; protein abundance increases in
response to DNA replication stress"
/codon_start=1
/product="putative hydrolase"
/protein_id="NP_014702.1"
/db_xref="GeneID:854225"
/db_xref="SGD:S000005585"
/translation="MTSDKHLFVLIHGLWGNYTHMESMRTILSTTLKKEDVNDDMIYF
LPKQNAMFKTFDGIEIIGYRTLIEVCEFIRDYKDGKITKLSVMGYSQGGLVARFMIGK
MLTEFKELFEDIEPQLFITMATPHLGVEFYNPTGIAYKSALYSALRTLGSTILGKSGR
EMFIANSSNNILVKLSQGEYLEALSLFKWRIAFANVKNDRTVAFYTAFITDCDPFIDF
DNKLKYTFEEKIPGSGYKGILPKIVDLNALNVNSHAPTKPTKTYKKWGRTILIILVAT
FLILPIALVMNGLGTAYSYIVTCKYRKMLSNGILHNEVRGKLGLTEQLKGYVTDAYGS
IINSALDMDANYEASNSNLVNEEELPWKEFIQKYTTINDGVWKSKFKKLPFDENRKVI
LRNLNKLKWIRVPIYIKAVNAHGVIVARRGMDENTAATGIACIEFTAQLLAYLMHKSN
"
gene complement(<440390..>441163)
/gene="SLD7"
/locus_tag="YOR060C"
/gene_synonym="YOR29-11"
/db_xref="GeneID:854226"
mRNA complement(<440390..>441163)
/gene="SLD7"
/locus_tag="YOR060C"
/gene_synonym="YOR29-11"
/product="Sld7p"
/transcript_id="NM_001183479.1"
/db_xref="GeneID:854226"
CDS complement(440390..441163)
/gene="SLD7"
/locus_tag="YOR060C"
/gene_synonym="YOR29-11"
/experiment="EXISTENCE:direct assay:GO:0000775 chromosome,
centromeric region [PMID:23746350]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095|PMID:26928762|PMID:14690591]"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:14690591]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14690591]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14690591]"
/experiment="EXISTENCE:direct assay:GO:0031261 DNA
replication preinitiation complex [PMID:21487389]"
/experiment="EXISTENCE:genetic interaction:GO:0030174
regulation of DNA-templated DNA replication initiation
[PMID:21487389]"
/experiment="EXISTENCE:physical interaction:GO:0030174
regulation of DNA-templated DNA replication initiation
[PMID:21487389]"
/note="Protein with a role in chromosomal DNA replication;
interacts with Sld3p and reduces its affinity for Cdc45p;
deletion mutant has aberrant mitochondria; ortholog of
human MTBP, which is a DNA replication origin firing
factor"
/codon_start=1
/product="Sld7p"
/protein_id="NP_014703.1"
/db_xref="GeneID:854226"
/db_xref="SGD:S000005586"
/translation="MSRKLCTLNFTLSGKQGSLVIRDIQLWSNRPTASKSTSELRGQF
IQYVDLAKLPLWVRSTNMNTYRCYSTSATAQAYFKSKLRNANRGIVIELFDKVDQRSQ
EPAYLIIFRENTELNCFQVDLTMKHEFDGQVTKLKQDIGKTRASVSKEGSIDIIIQQS
QQRKIGTKTEVYRNVHINDKRLQFNETLSKLILGGLRLRGISNSITDYQKLYKITFDA
AEFTHRDELKRISMGSGEEVSFESLQETVETLLKLFTKS"
gene <441534..>442553
/gene="CKA2"
/locus_tag="YOR061W"
/gene_synonym="YOR29-12"
/db_xref="GeneID:854227"
mRNA <441534..>442553
/gene="CKA2"
/locus_tag="YOR061W"
/gene_synonym="YOR29-12"
/product="casein kinase 2 catalytic subunit CKA2"
/transcript_id="NM_001183480.1"
/db_xref="GeneID:854227"
CDS 441534..442553
/gene="CKA2"
/locus_tag="YOR061W"
/gene_synonym="YOR29-12"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004674 protein
serine/threonine kinase activity [PMID:8226802]"
/experiment="EXISTENCE:direct assay:GO:0005956 protein
kinase CK2 complex [PMID:8135547]"
/experiment="EXISTENCE:direct assay:GO:0006356 regulation
of transcription by RNA polymerase I [PMID:11551505]"
/experiment="EXISTENCE:direct assay:GO:0006359 regulation
of transcription by RNA polymerase III [PMID:11551505]"
/experiment="EXISTENCE:direct assay:GO:0006974 DNA damage
response [PMID:11551505]"
/experiment="EXISTENCE:direct assay:GO:0034456 UTP-C
complex [PMID:17515605]"
/experiment="EXISTENCE:genetic interaction:GO:0007535
donor selection [PMID:22496671]"
/note="Alpha' catalytic subunit of casein kinase 2 (CK2);
CK2 is a Ser/Thr protein kinase with roles in cell growth
and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and
CKB2, has many substrates including transcription factors
and all RNA polymerases; protein abundance increases in
response to DNA replication stress; regulates
Fkh1p-mediated donor preference during mating-type
switching"
/codon_start=1
/product="casein kinase 2 catalytic subunit CKA2"
/protein_id="NP_014704.1"
/db_xref="GeneID:854227"
/db_xref="SGD:S000005587"
/translation="MPLPPSTLNQKSNRVYSVARVYKNACEERPQEYWDYEQGVTIDW
GKISNYEIINKIGRGKYSEVFSGRCIVNNQKCVIKVLKPVKMKKIYRELKILTNLTGG
PNVVGLYDIVQDADSKIPALIFEEIKNVDFRTLYPTFKLPDIQYYFTQLLIALDYCHS
MGIMHRDVKPQNVMIDPTERKLRLIDWGLAEFYHPGVDYNVRVASRYHKGPELLVNLN
QYDYSLDLWSVGCMLAAIVFKKEPFFKGSSNPDQLVKIATVLGTKELLGYLGKYGLHL
PSEYDNIMRDFTKKSWTHFITSETKLAVPEVVDLIDNLLRYDHQERLTAKEAMDHKFF
KTKFE"
gene complement(<442725..>443531)
/locus_tag="YOR062C"
/gene_synonym="YOR29-13"
/db_xref="GeneID:854228"
mRNA complement(<442725..>443531)
/locus_tag="YOR062C"
/gene_synonym="YOR29-13"
/product="uncharacterized protein"
/transcript_id="NM_001183481.1"
/db_xref="GeneID:854228"
CDS complement(442725..443531)
/locus_tag="YOR062C"
/gene_synonym="YOR29-13"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="hypothetical protein; similar to Reg1p; expression
regulated by glucose and Rgt1p; GFP-fusion protein is
induced in response to the DNA-damaging agent MMS; YOR062C
has a paralog, YKR075C, that arose from the whole genome
duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014705.1"
/db_xref="GeneID:854228"
/db_xref="SGD:S000005588"
/translation="MTSLDDSVLTKKNIALLDNATNYIRPAIDYFHFKFNYDSLDVST
TWRLLLKMRKHKLLRLPSCSSENEFDYSIYMARLYHCIWRRWSIKHFNLDEYKIDPLS
INWNKEIDVTVLYGPDLVGIHEREQPTPTDFPMGNIKEQGKQLLDVRKEGSASSLLKK
GSVFYSKGKWLSQRSISFDDTVRRRDIDKRGRFRESCVLINDVEQFQNYSIVWDESRH
RYRRQALPDTYDYEHLYPNGDETPRNTPHDNIIIHQNLHSITEGSYIYIK"
gene <444686..>445849
/gene="RPL3"
/locus_tag="YOR063W"
/gene_synonym="MAK8; TCM1; YOR29-14"
/db_xref="GeneID:854229"
mRNA <444686..>445849
/gene="RPL3"
/locus_tag="YOR063W"
/gene_synonym="MAK8; TCM1; YOR29-14"
/product="60S ribosomal protein uL3 RPL3"
/transcript_id="NM_001183482.1"
/db_xref="GeneID:854229"
CDS 444686..445849
/gene="RPL3"
/locus_tag="YOR063W"
/gene_synonym="MAK8; TCM1; YOR29-14"
/experiment="EXISTENCE:curator inference:GO:0003735
structural constituent of ribosome [PMID:11983894]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:11983894]"
/experiment="EXISTENCE:mutant phenotype:GO:0000027
ribosomal large subunit assembly [PMID:3537704]"
/experiment="EXISTENCE:mutant phenotype:GO:0006364 rRNA
processing [PMID:26826131]"
/experiment="EXISTENCE:mutant phenotype:GO:0006414
translational elongation [PMID:26826131]"
/experiment="EXISTENCE:mutant phenotype:GO:1990145
maintenance of translational fidelity
[PMID:9858562|PMID:26826131]"
/note="Ribosomal 60S subunit protein L3; homologous to
mammalian ribosomal protein L3 and bacterial L3; plays an
important role in function of eIF5B in stimulating 3' end
processing of 18S rRNA in context of 80S ribosomes that
have not yet engaged in translation; involved in
replication and maintenance of killer double stranded RNA
virus"
/codon_start=1
/product="60S ribosomal protein uL3 RPL3"
/protein_id="NP_014706.1"
/db_xref="GeneID:854229"
/db_xref="SGD:S000005589"
/translation="MSHRKYEAPRHGHLGFLPRKRAASIRARVKAFPKDDRSKPVALT
SFLGYKAGMTTIVRDLDRPGSKFHKREVVEAVTVVDTPPVVVVGVVGYVETPRGLRSL
TTVWAEHLSDEVKRRFYKNWYKSKKKAFTKYSAKYAQDGAGIERELARIKKYASVVRV
LVHTQIRKTPLAQKKAHLAEIQLNGGSISEKVDWAREHFEKTVAVDSVFEQNEMIDAI
AVTKGHGFEGVTHRWGTKKLPRKTHRGLRKVACIGAWHPAHVMWSVARAGQRGYHSRT
SINHKIYRVGKGDDEANGATSFDRTKKTITPMGGFVHYGEIKNDFIMVKGCIPGNRKR
IVTLRKSLYTNTSRKALEEVSLKWIDTASKFGKGRFQTPAEKHAFMGTLKKDL"
gene complement(<446079..>446738)
/gene="YNG1"
/locus_tag="YOR064C"
/gene_synonym="YOR29-15"
/db_xref="GeneID:854230"
mRNA complement(<446079..>446738)
/gene="YNG1"
/locus_tag="YOR064C"
/gene_synonym="YOR29-15"
/product="Yng1p"
/transcript_id="NM_001183483.1"
/db_xref="GeneID:854230"
CDS complement(446079..446738)
/gene="YNG1"
/locus_tag="YOR064C"
/gene_synonym="YOR29-15"
/experiment="EXISTENCE:direct assay:GO:0033100 NuA3
histone acetyltransferase complex [PMID:17157260]"
/experiment="EXISTENCE:direct assay:GO:0140002 histone
H3K4me3 reader activity [PMID:17157260|PMID:16923967]"
/experiment="EXISTENCE:direct assay:GO:1990467 NuA3a
histone acetyltransferase complex [PMID:25104842]"
/experiment="EXISTENCE:genetic interaction:GO:0032968
positive regulation of transcription elongation by RNA
polymerase II [PMID:25104842]"
/experiment="EXISTENCE:mutant phenotype:GO:0004402 histone
acetyltransferase activity [PMID:12077334]"
/experiment="EXISTENCE:mutant phenotype:GO:0006325
chromatin organization [PMID:10805724]"
/experiment="EXISTENCE:mutant phenotype:GO:0140002 histone
H3K4me3 reader activity [PMID:17157260]"
/experiment="EXISTENCE:physical interaction:GO:0033100
NuA3 histone acetyltransferase complex [PMID:12077334]"
/note="Subunit of the NuA3 histone acetyltransferase
complex; this complex acetylates histone H3; contains PHD
finger domain that interacts with methylated histone H3;
shares significant sequence identity with the human
candidate tumor suppressor p33-ING1 in C-terminal region"
/codon_start=1
/product="Yng1p"
/protein_id="NP_014707.1"
/db_xref="GeneID:854230"
/db_xref="SGD:S000005590"
/translation="MEHLANENSDSDIRYSFLSTLDHLPCELIRSLRLMQTIDLFKNE
EDEPGMERACRDLLLVATYINDLVDDQIHFLKQHKKELEIQKSVTKNFNSSLENIKSK
LTLEEPGAYKEPKLLLKINLKKAKSRERKESITSPTIGINQGDVTEGNNNQEEVYCFC
RNVSYGPMVACDNPACPFEWFHYGCVGLKQAPKGKWYCSKDCKEIANQRSKSKRQKRR
K"
gene <447439..>448368
/gene="CYT1"
/locus_tag="YOR065W"
/gene_synonym="CTC1; YOR29-16"
/db_xref="GeneID:854231"
mRNA <447439..>448368
/gene="CYT1"
/locus_tag="YOR065W"
/gene_synonym="CTC1; YOR29-16"
/product="ubiquinol--cytochrome-c reductase catalytic
subunit CYT1"
/transcript_id="NM_001183484.1"
/db_xref="GeneID:854231"
CDS 447439..448368
/gene="CYT1"
/locus_tag="YOR065W"
/gene_synonym="CTC1; YOR29-16"
/EC_number="7.1.1.8"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0045275 respiratory
chain complex III [PMID:10873857]"
/experiment="EXISTENCE:mutant phenotype:GO:0006122
mitochondrial electron transport, ubiquinol to cytochrome
c [PMID:11279090]"
/experiment="EXISTENCE:mutant phenotype:GO:0008121
quinol-cytochrome-c reductase activity [PMID:11279090]"
/note="Cytochrome c1; component of the mitochondrial
respiratory chain; expression is regulated by the
heme-activated, glucose-repressed Hap2p/3p/4p/5p
CCAAT-binding complex"
/codon_start=1
/product="ubiquinol--cytochrome-c reductase catalytic
subunit CYT1"
/protein_id="NP_014708.1"
/db_xref="GeneID:854231"
/db_xref="SGD:S000005591"
/translation="MFSNLSKRWAQRTLSKSFYSTATGAASKSGKLTQKLVTAGVAAA
GITASTLLYADSLTAEAMTAAEHGLHAPAYAWSHNGPFETFDHASIRRGYQVYREVCA
ACHSLDRVAWRTLVGVSHTNEEVRNMAEEFEYDDEPDEQGNPKKRPGKLSDYIPGPYP
NEQAARAANQGALPPDLSLIVKARHGGCDYIFSLLTGYPDEPPAGVALPPGSNYNPYF
PGGSIAMARVLFDDMVEYEDGTPATTSQMAKDVTTFLNWCAEPEHDERKRLGLKTVII
LSSLYLLSIWVKKFKWAGIKTRKFVFNPPKPRK"
gene <449436..>451325
/gene="MSA1"
/locus_tag="YOR066W"
/gene_synonym="YOR29-17"
/db_xref="GeneID:854232"
mRNA <449436..>451325
/gene="MSA1"
/locus_tag="YOR066W"
/gene_synonym="YOR29-17"
/product="Msa1p"
/transcript_id="NM_001183485.1"
/db_xref="GeneID:854232"
CDS 449436..451325
/gene="MSA1"
/locus_tag="YOR066W"
/gene_synonym="YOR29-17"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:19520826]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:19520826]"
/experiment="EXISTENCE:genetic interaction:GO:0007089
traversing start control point of mitotic cell cycle
[PMID:18160399]"
/experiment="EXISTENCE:mutant phenotype:GO:0000082 G1/S
transition of mitotic cell cycle [PMID:18160399]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:18160399]"
/experiment="EXISTENCE:mutant phenotype:GO:0007089
traversing start control point of mitotic cell cycle
[PMID:18160399]"
/experiment="EXISTENCE:physical interaction:GO:0000082
G1/S transition of mitotic cell cycle [PMID:18160399]"
/experiment="EXISTENCE:physical interaction:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:18160399]"
/note="Activator of G1-specific transcription factors MBF
and SBF; involved in regulation of the timing of
G1-specific gene transcription and cell cycle initiation;
localization is cell-cycle dependent and regulated by
Cdc28p phosphorylation; MSA1 has a paralog, MSA2, that
arose from the whole genome duplication"
/codon_start=1
/product="Msa1p"
/protein_id="NP_014709.1"
/db_xref="GeneID:854232"
/db_xref="SGD:S000005592"
/translation="MDKSMIKKRGRPPITKDYPNPLQSPMAHSSMQVQKQGPHSFAKP
LMKVGQSSPSPNKRRLSIDHHHNLAATTRKGRYRGVLLSTPTKKSSTNGSTPISTPSS
NDSYNNTVFSETRKTFLQSSPPIMTSSPAFQKKNDYMFPSQEQFKLSLTITESGKAVI
AGSLPFSPSSKSSHLMNNNNKKIMQNEKIHKGSKKNAPKFEKRRILSLLKQMKNEKYC
DTDTLPEAPPAKPSRSDIIDTELPTIIETSASPIGSARNNNILLSQPPQSPPSSAQLK
PPSTPKSSLQFRMGFTPNVALNSVSLSDTISKSTNAVGASNNNNQNGNSISNIADANT
LLTLTNSPGVFLSPRNKMLPKSTTASNEQQQEFVFKFSSGDPLLLTDDADGNWPEMLF
NVSNTPRRQKCFNTPPSWINFGSPGLFSPPRSSNVMVNGTTVATASDSGNVHRQLQAQ
LEAQVQVQSQSNSPTQRQQQQRQFQIPPPHINMNSSPPQINIASPPHQSMSRVSSIYF
NKEKTTTGVANMLGNTKSENLQPPANLFTAAHGPSTPRNQEFQLPTLIECTPLIQQTM
NGSLGTKYIPGTSISNSATPNLHGFPVGTGKAPSSFDDSLKQNPYSNKQDDARTALKR
LIDDQ"
gene complement(<451729..>453462)
/gene="ALG8"
/locus_tag="YOR067C"
/gene_synonym="YOR29-18"
/db_xref="GeneID:854233"
mRNA complement(<451729..>453462)
/gene="ALG8"
/locus_tag="YOR067C"
/gene_synonym="YOR29-18"
/product="dolichyl-P-Glc:Glc1Man(9)GlcNAc(2)-PP-dolichol
alpha-1,3-glucosyltransferase"
/transcript_id="NM_001183486.1"
/db_xref="GeneID:854233"
CDS complement(451729..453462)
/gene="ALG8"
/locus_tag="YOR067C"
/gene_synonym="YOR29-18"
/EC_number="2.4.1.265"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0006487
protein N-linked glycosylation [PMID:8016100]"
/experiment="EXISTENCE:mutant phenotype:GO:0005789
endoplasmic reticulum membrane [PMID:8016100]"
/experiment="EXISTENCE:mutant phenotype:GO:0006488
dolichol-linked oligosaccharide biosynthetic process
[PMID:3536907]"
/experiment="EXISTENCE:mutant phenotype:GO:0042283
dolichyl pyrophosphate Glc1Man9GlcNAc2
alpha-1,3-glucosyltransferase activity [PMID:3536907]"
/note="Glucosyl transferase; involved in N-linked
glycosylation; adds glucose to the dolichol-linked
oligosaccharide precursor prior to transfer to protein
during lipid-linked oligosaccharide biosynthesis; similar
to Alg6p; human homolog ALG8 can complement yeast null
mutant"
/codon_start=1
/product="dolichyl-P-Glc:Glc1Man(9)GlcNAc(2)-PP-dolichol
alpha-1,3-glucosyltransferase"
/protein_id="NP_014710.1"
/db_xref="GeneID:854233"
/db_xref="SGD:S000005593"
/translation="MKGDRSRQNMAVTKKAKLKKNDEPKKVLKTAATEKGEGSKRYSL
WNFWISTLFLKLLLIPDYFSTDFDVHRNWLAITNKLPISEWYYEHTSQWTLDYPPFFA
YFEWFLSQFVPKSVRDDGCLDIVEIGKFGLPTIVFQRLTVIFSEILLFVILQIYINTT
KLSERSQSFVVASSIVLSPGFLIIDHIHFQYNGFLFAILIGSIVAAKNKRYILCAVLY
TTAICFKHIFLYLAPCYFVFLLRAYVLNVNNFKFKSYKDFLFLIRWANLLKLATVVVG
IFTICFLPFAHQMPQVLSRLFPFSRGLTHAYWAPNFWALYSFMDKILTTVMLKLPYVH
TFATKFIKPPLIPQNIKEINERLAANNNGSKGLVQDVFFVILPQIPPKLTFILTIFYQ
VLAVLPLLFDPSFKRFVGSLTLCGLASFLFGWHVHEKAIMLVIIPFTFLVGFDRRLLV
PFMLVASAGYVSLYPLLYKGQDFFIKTLYTYVWCIIYFAAFRKTTKISSSVERRIFFL
DRLALTYIFSLLPIVTVLQILDEVKWRYSFLQKFEFLGLMIYSVYCSLGIISSWFALS
WLYNFDELLWQ"
gene <453768..>455795
/gene="VPS5"
/locus_tag="YOR069W"
/gene_synonym="GRD2; PEP10; VPT5"
/db_xref="GeneID:854235"
mRNA <453768..>455795
/gene="VPS5"
/locus_tag="YOR069W"
/gene_synonym="GRD2; PEP10; VPT5"
/product="sorting nexin 1"
/transcript_id="NM_001183488.1"
/db_xref="GeneID:854235"
CDS 453768..455795
/gene="VPS5"
/locus_tag="YOR069W"
/gene_synonym="GRD2; PEP10; VPT5"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:9175702|PMID:9700157]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:9175702]"
/experiment="EXISTENCE:direct assay:GO:0032266
phosphatidylinositol-3-phosphate binding [PMID:11557775]"
/experiment="EXISTENCE:mutant phenotype:GO:0030904
retromer complex [PMID:9700157]"
/experiment="EXISTENCE:mutant phenotype:GO:0045053 protein
retention in Golgi apparatus [PMID:8649377]"
/experiment="EXISTENCE:mutant phenotype:GO:0140312 cargo
adaptor activity [PMID:9700157]"
/experiment="EXISTENCE:physical interaction:GO:0030904
retromer complex [PMID:9700157]"
/experiment="EXISTENCE:physical interaction:GO:0030905
retromer, tubulation complex [PMID:9700157]"
/experiment="EXISTENCE:physical interaction:GO:0042147
retrograde transport, endosome to Golgi [PMID:9700157]"
/experiment="EXISTENCE:physical interaction:GO:0140312
cargo adaptor activity [PMID:9700157]"
/note="Nexin-1 homolog; required for localizing membrane
proteins from a prevacuolar/late endosomal compartment
back to late Golgi; structural component of retromer
membrane coat complex; forms a retromer subcomplex with
Vps17p; required for recruiting the retromer complex to
the endosome membranes; VPS5 has a paralog, YKR078W, that
arose from the whole genome duplication"
/codon_start=1
/product="sorting nexin 1"
/protein_id="NP_014712.2"
/db_xref="GeneID:854235"
/db_xref="SGD:S000005595"
/translation="MDYEDNLEAPVWDELNHEGDKTQSLVSNSIESIGQISTNEERKD
NELLETTASFADKIDLDSAPEWKDPGLSVAGNPQLEEHDNSKADDLINSLAPEQDPIA
DLKNSTTQFIATRESGGALFTGNANSPLVFDDTIYDANTSPNTSKSISGRRSGKPRIL
FDSARAQRNSKRNHSLKAKRTTASDDTIKTPFTDPLKKAEKENEFVEEPLDDRNERRE
NNEGKFTASVEKNILEQVDRPLYNLPQTGANISSPAEVEENSEKFGKTKIGSKVPPTE
KAVAFKVEVKDPVKVGELTSIHVEYTVISESSLLELKYAQVSRRYRDFRWLYRQLQNN
HWGKVIPPPPEKQSVGSFKENFIENRRFQMESMLKKICQDPVLQKDKDFLLFLTSDDF
SSESKKRAFLTGSGAINDSNDLSEVRISEIQLLGAEDAAEVLKNGGIDAESHKGFMSI
SFSSLPKYNEADEFFIEKKQKIDELEDNLKKLSKSLEMVDTSRNTLAASTEEFSSMVE
TLASLNVSEPNSELLNNFADVHKSIKSSLERSSLQETLTMGVMLDDYIRSLASVKAIF
NQRSKLGYFLVVIENDMNKKHSQLGKLGQNIHSEKFREMRKEFQTLERRYNLTKKQWQ
AVGDKIKDEFQGFSTDKIREFRNGMEISLEAAIESQKECIELWETFYQTNL"
gene complement(<453869..>454213)
/gene="VAM10"
/locus_tag="YOR068C"
/db_xref="GeneID:854234"
mRNA complement(<453869..>454213)
/gene="VAM10"
/locus_tag="YOR068C"
/product="Vam10p"
/transcript_id="NM_001183487.1"
/db_xref="GeneID:854234"
CDS complement(453869..454213)
/gene="VAM10"
/locus_tag="YOR068C"
/experiment="EXISTENCE:curator inference:GO:0000329
fungal-type vacuole membrane [PMID:12748377]"
/experiment="EXISTENCE:mutant phenotype:GO:0042144 vacuole
fusion, non-autophagic [PMID:12748377]"
/note="Protein involved in vacuole morphogenesis; acts at
an early step of homotypic vacuole fusion that is required
for vacuole tethering"
/codon_start=1
/product="Vam10p"
/protein_id="NP_014711.1"
/db_xref="GeneID:854234"
/db_xref="SGD:S000005594"
/translation="MLFEVFGEVLASYIVSSKTKGELAFPVNNAPPDSLVAINCVVLF
LRSAIGSCSGAKELIRSSALELSCSSSCGLPATDKPGSFHSGALSKSILSANEAVVSK
SSLSFLSSFVDI"
gene complement(<455907..>457820)
/gene="GYP1"
/locus_tag="YOR070C"
/gene_synonym="YOR29-21"
/db_xref="GeneID:854236"
mRNA complement(<455907..>457820)
/gene="GYP1"
/locus_tag="YOR070C"
/gene_synonym="YOR29-21"
/product="GTPase-activating protein GYP1"
/transcript_id="NM_001183489.1"
/db_xref="GeneID:854236"
CDS complement(455907..457820)
/gene="GYP1"
/locus_tag="YOR070C"
/gene_synonym="YOR29-21"
/experiment="EXISTENCE:direct assay:GO:0005096 GTPase
activator activity [PMID:10508155]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:35134079]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:11359917]"
/experiment="EXISTENCE:direct assay:GO:0016192
vesicle-mediated transport [PMID:11118206]"
/experiment="EXISTENCE:mutant phenotype:GO:0005096 GTPase
activator activity [PMID:10508155]"
/note="Cis-golgi GTPase-activating protein (GAP) for yeast
Rabs; the Rab family members are Ypt1p (in vivo) and for
Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in
vesicle docking and fusion; interacts with autophagosome
component Atg8p"
/codon_start=1
/product="GTPase-activating protein GYP1"
/protein_id="NP_014713.1"
/db_xref="GeneID:854236"
/db_xref="SGD:S000005596"
/translation="MGVRSAAKEMHERDHNSDSSSLVTSLMKSWRISSASSSKKPSLY
KMNTTESTSLPSGYASSADRDRRTSDGNFEAMAKQQASTRRTSNSYSPLRYVNPTLST
ASNESPRPALLLRQHHQRHHHHQQPRHSSSGSVGNNCSNSTEPNKKGDRYFKDLDEDW
SAVIDDYNMPIPILTNGGFGTPVAPTRTLSRKSTSSSINSISNMGTSAVRNSSSSFTY
PQLPQLQKEKTNDSKKTQLEIENERDVQELNSIIQRISKFDNILKDKTIINQQDLRQI
SWNGIPKIHRPVVWKLLIGYLPVNTKRQEGFLQRKRKEYRDSLKHTFSDQHSRDIPTW
HQIEIDIPRTNPHIPLYQFKSVQNSLQRILYLWAIRHPASGYVQGINDLVTPFFETFL
TEYLPPSQIDDVEIKDPSTYMVDEQITDLEADTFWCLTKLLEQITDNYIHGQPGILRQ
VKNLSQLVKRIDADLYNHFQNEHVEFIQFAFRWMNCLLMREFQMGTVIRMWDTYLSET
SQEVTSSYSMSSNDIKPPVTPTEPRVASFVTPTKDFQSPTTALSNMTPNNAVEDSGKM
RQSSLNEFHVFVCAAFLIKWSDQLMEMDFQETITFLQNPPTKDWTETDIEMLLSEAFI
WQSLYKDATSHWL"
gene complement(<459480..>461276)
/gene="NRT1"
/locus_tag="YOR071C"
/gene_synonym="THI71; YOR29-22"
/db_xref="GeneID:854237"
mRNA complement(<459480..>461276)
/gene="NRT1"
/locus_tag="YOR071C"
/gene_synonym="THI71; YOR29-22"
/product="nicotinamide riboside transporter"
/transcript_id="NM_001183490.1"
/db_xref="GeneID:854237"
CDS complement(459480..461276)
/gene="NRT1"
/locus_tag="YOR071C"
/gene_synonym="THI71; YOR29-22"
/experiment="EXISTENCE:genetic interaction:GO:0015888
thiamine transport [PMID:16850348|PMID:9235906]"
/experiment="EXISTENCE:genetic interaction:GO:0034257
nicotinamide riboside transmembrane transporter activity
[PMID:18258590]"
/experiment="EXISTENCE:genetic interaction:GO:0034258
nicotinamide riboside transport [PMID:18258590]"
/experiment="EXISTENCE:mutant phenotype:GO:0034257
nicotinamide riboside transmembrane transporter activity
[PMID:18258590]"
/experiment="EXISTENCE:mutant phenotype:GO:0034258
nicotinamide riboside transport [PMID:18258590]"
/experiment="EXISTENCE:mutant phenotype:GO:1903088
5-amino-1-ribofuranosylimidazole-4-carboxamide
transmembrane transport [PMID:24778186]"
/experiment="EXISTENCE:mutant phenotype:GO:1903089
5-amino-1-ribofuranosylimidazole-4-carboxamide
transmembrane transporter activity [PMID:24778186]"
/note="High-affinity nicotinamide riboside transporter;
also transports thiamine with low affinity; major
transporter for
5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside
(acadesine) uptake; shares sequence similarity with Thi7p
and Thi72p; proposed to be involved in 5-fluorocytosine
sensitivity"
/codon_start=1
/product="nicotinamide riboside transporter"
/protein_id="NP_014714.1"
/db_xref="GeneID:854237"
/db_xref="SGD:S000005597"
/translation="MSFSSIVSKFLRYLEIPAKNRTAVNFLRNPDLQPIKSANQTWGF
WSNLAYWGAVSFTAGTWMSGSAALSVGLSYPETIVSFLLGNVLTIIFTMANSYPGYDW
KIGFTLAQRFVFGIYGSAFGIIIRILMSIVNYGSNAWLGGLSINMILDSWSHHYLHLP
NTLSPSVAMTTKQLVGFIIFHVLTALCYFMKPYHMNYLLIWSCVATCFAMLGIVIYLT
KNAHGVGELFTSTKSTVTGSKRAWAWVYMISYWFGSISPGSTNQSDYSRFGSSNLAIW
TGSVCALLIPATLVPIFGVISASTCDKLYGKQFWMPMDIFDYWLTNNYSAGARAGAFF
CGLCFTMSQMSSTISNCGFATGMDMAGLLPKYVDIKRGALFCACISWACLPWNFYNSS
STFLTVMSSFGVVMTPIIAVMICDNFLIRKRQYSITNAFILKGEYYFTKGVNWRAIVA
WVCGMAPGLPGIAWEVNNNYFHDSGIVKFFYGDSFFSFLISFFVYWGLCVFFPFKITV
RHDDKDYYGAFTDEEARKKGMIPYSEISEEEIRAYTLGECYTTGHEYKPESSDNESPE
LIKTSSENTNVFEIVHQKDDEKHSFSTTQQVV"
gene <461502..>461816
/locus_tag="YOR072W"
/gene_synonym="YOR29-23"
/db_xref="GeneID:854238"
mRNA <461502..>461816
/locus_tag="YOR072W"
/gene_synonym="YOR29-23"
/product="uncharacterized protein"
/transcript_id="NM_001348878.1"
/db_xref="GeneID:854238"
CDS 461502..461816
/locus_tag="YOR072W"
/gene_synonym="YOR29-23"
/note="hypothetical protein; conserved across S.
cerevisiae strains; partially overlaps the dubious gene
YOR072W-A; diploid deletion strains are methotrexate,
paraquat and wortmannin sensitive"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335816.1"
/db_xref="GeneID:854238"
/db_xref="SGD:S000005598"
/translation="MLTKVVFLFFWSRSDSTKKLAACNHATLAHYILTPALYSDACAI
YSDVYSITIIVVATVVRNPARCSLRARKFCRLFSSFFQFHYLKELFYFIRKPDDKFSS
FI"
gene 464450..464551
/gene="SUF11"
/locus_tag="YNCO0017W"
/db_xref="GeneID:854239"
tRNA join(464450..464485,464516..464551)
/gene="SUF11"
/locus_tag="YNCO0017W"
/product="tRNA-Pro"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE
analysis; target of K. lactis zymocin; can mutate to
suppress +1 frameshift mutations in proline codons"
/db_xref="GeneID:854239"
/db_xref="SGD:S000006688"
gene <464469..>464630
/locus_tag="YOR072W-B"
/db_xref="GeneID:1466483"
mRNA <464469..>464630
/locus_tag="YOR072W-B"
/product="uncharacterized protein"
/transcript_id="NM_001184526.1"
/db_xref="GeneID:1466483"
CDS 464469..464630
/locus_tag="YOR072W-B"
/note="hypothetical protein; identified by expression
profiling and mass spectrometry"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878171.1"
/db_xref="GeneID:1466483"
/db_xref="SGD:S000028516"
/translation="MILALGDFLTNRKTKHARGPGFNSQLAPFIFDYLFPIGRVTDFF
YFFQGPFVL"
gene <464771..>466543
/gene="SGO1"
/locus_tag="YOR073W"
/gene_synonym="YOR29-24"
/db_xref="GeneID:854240"
mRNA <464771..>466543
/gene="SGO1"
/locus_tag="YOR073W"
/gene_synonym="YOR29-24"
/product="Sgo1p"
/transcript_id="NM_001183492.1"
/db_xref="GeneID:854240"
CDS 464771..466543
/gene="SGO1"
/locus_tag="YOR073W"
/gene_synonym="YOR29-24"
/experiment="EXISTENCE:direct assay:GO:0000775 chromosome,
centromeric region [PMID:25236599]"
/experiment="EXISTENCE:direct assay:GO:0000776 kinetochore
[PMID:14730319|PMID:15062096]"
/experiment="EXISTENCE:direct assay:GO:0000779 condensed
chromosome, centromeric region
[PMID:28980861|PMID:16357219|PMID:14730319]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0045184
establishment of protein localization [PMID:25236599]"
/experiment="EXISTENCE:direct assay:GO:0051383 kinetochore
organization [PMID:22365852]"
/experiment="EXISTENCE:genetic interaction:GO:0000070
mitotic sister chromatid segregation [PMID:15637284]"
/experiment="EXISTENCE:genetic interaction:GO:0007094
mitotic spindle assembly checkpoint signaling
[PMID:15637284]"
/experiment="EXISTENCE:genetic interaction:GO:0034096
positive regulation of maintenance of meiotic sister
chromatid cohesion [PMID:15062096]"
/experiment="EXISTENCE:mutant phenotype:GO:0000070 mitotic
sister chromatid segregation
[PMID:15637284|PMID:14730319|PMID:15062096]"
/experiment="EXISTENCE:mutant phenotype:GO:0000278 mitotic
cell cycle [PMID:24945276]"
/experiment="EXISTENCE:mutant phenotype:GO:0000779
condensed chromosome, centromeric region [PMID:28980861]"
/experiment="EXISTENCE:mutant phenotype:GO:0007094 mitotic
spindle assembly checkpoint signaling [PMID:15637284]"
/experiment="EXISTENCE:mutant phenotype:GO:0031134 sister
chromatid biorientation
[PMID:25236599|PMID:18081426|PMID:17980598]"
/experiment="EXISTENCE:mutant phenotype:GO:0034090
maintenance of meiotic sister chromatid cohesion
[PMID:14752166]"
/experiment="EXISTENCE:mutant phenotype:GO:0034096
positive regulation of maintenance of meiotic sister
chromatid cohesion [PMID:15062096]"
/experiment="EXISTENCE:mutant phenotype:GO:0045144 meiotic
sister chromatid segregation
[PMID:15062096|PMID:14730319]"
/experiment="EXISTENCE:mutant phenotype:GO:0045184
establishment of protein localization [PMID:25236599]"
/experiment="EXISTENCE:mutant phenotype:GO:0051383
kinetochore organization [PMID:22365852]"
/experiment="EXISTENCE:mutant phenotype:GO:0051757 meiotic
sister chromatid separation [PMID:15062096]"
/experiment="EXISTENCE:mutant phenotype:GO:0070199
establishment of protein localization to chromosome
[PMID:24945276]"
/note="Component of the spindle checkpoint; involved in
sensing lack of tension on mitotic chromosomes; protects
centromeric Rec8p at meiosis I; required for accurate
chromosomal segregation at meiosis II and for mitotic
chromosome stability; recruits condensin to the
pericentric region of chromosomes during meiosis;
dissociates from pericentromeres when sister kinetochores
are under tension"
/codon_start=1
/product="Sgo1p"
/protein_id="NP_014716.1"
/db_xref="GeneID:854240"
/db_xref="SGD:S000005599"
/translation="MPKRKIAPNKESSRRTVSHDDLTPQIQEFQNLMDLESQKVENIR
QSYSRQNSLLAKDNSILKIKVNSLEKKISQLVQENVTLRSKTSISEAIYRERLSNQLQ
VIENGIIQRFDEIFYMFENVRKNENLPSSSLRTMLKRTSSRSRSCSLSSPTYSKSYTR
LSNHENNLSHESSFNKDDGPDLEPKAKKRKSSRRQSMFVSTSLEPEDETGENEPMMEN
SSVEVPAESHESAQVEETIDALNPEEENSDSVSNFTNSIIEYSIPEENPTEPEHSSSK
LEIFNDSTNMLSTVPSNPLPLPLPGPSATLPTTTSDASTVYPSSSSSTNSHPKTKIKH
SMKPPRIELKKKVIDEVMPVSNMSSNSEISFTRTRRTRGKAVDYTLPSLRAKMRRPSE
KLVDATTVIDIHDLQVSKRNRETSHKRKSLSQDSIPDEPQLREVVVSKDYGTPKGKKT
EDEIHEDTAHLMTTSNNNSNNKNEKKLTSNNSPKKSSPLLDITNKSENKKKSTRTKKL
FKNAIVNNLSDENSTTRPSKSSKGTSNNNNNYNNFDNNNSNINNVNNKSVSFRLNEDD
LAVFDLFGNGKAVKHQPKTYRTKK"
gene complement(<466675..>467589)
/gene="CDC21"
/locus_tag="YOR074C"
/gene_synonym="CRT9; TMP1"
/db_xref="GeneID:854241"
mRNA complement(<466675..>467589)
/gene="CDC21"
/locus_tag="YOR074C"
/gene_synonym="CRT9; TMP1"
/product="thymidylate synthase"
/transcript_id="NM_001183493.1"
/db_xref="GeneID:854241"
CDS complement(466675..467589)
/gene="CDC21"
/locus_tag="YOR074C"
/gene_synonym="CRT9; TMP1"
/EC_number="2.1.1.45"
/experiment="EXISTENCE:direct assay:GO:0004799 thymidylate
synthase activity [PMID:6287238]"
/experiment="EXISTENCE:direct assay:GO:0034399 nuclear
periphery [PMID:8132557]"
/experiment="EXISTENCE:mutant phenotype:GO:0004799
thymidylate synthase activity [PMID:334734]"
/experiment="EXISTENCE:mutant phenotype:GO:0006231 dTMP
biosynthetic process [PMID:794696|PMID:334734]"
/note="Thymidylate synthase; required for de novo
biosynthesis of pyrimidine deoxyribonucleotides;
expression is induced at G1/S; human homolog TYMSOS can
complement yeast cdc21 temperature-sensitive mutant at
restrictive temperature"
/codon_start=1
/product="thymidylate synthase"
/protein_id="NP_014717.2"
/db_xref="GeneID:854241"
/db_xref="SGD:S000005600"
/translation="MTMDGKNKEEEQYLDLCKRIIDEGEFRPDRTGTGTLSLFAPPQL
RFSLRDDTFPLLTTKKVFTRGIILELLWFLAGDTDANLLSEQGVKIWDGNGSREYLDK
MGFKDRKVGDLGPVYGFQWRHFGAKYKTCDDDYTGQGIDQLKQVIHKLKTNPYDRRII
MSAWNPADFDKMALPPCHIFSQFYVSFPKEGEGSGKPRLSCLLYQRSCDMGLGVPFNI
ASYALLTRMIAKVVDMEPGEFIHTLGDAHVYKDHIDALKEQITRNPRPFPKLKIKRDV
KDIDDFKLTDFEIEDYNPHPRIQMKMSV"
gene <468212..>469252
/gene="UFE1"
/locus_tag="YOR075W"
/db_xref="GeneID:854242"
mRNA <468212..>469252
/gene="UFE1"
/locus_tag="YOR075W"
/product="Ufe1p"
/transcript_id="NM_001183494.1"
/db_xref="GeneID:854242"
CDS 468212..469252
/gene="UFE1"
/locus_tag="YOR075W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:8612273|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0031201 SNARE
complex [PMID:9214619]"
/experiment="EXISTENCE:mutant phenotype:GO:0006890
retrograde vesicle-mediated transport, Golgi to
endoplasmic reticulum [PMID:8612273]"
/experiment="EXISTENCE:mutant phenotype:GO:0016320
endoplasmic reticulum membrane fusion [PMID:9506516]"
/note="t-SNARE protein required for retrograde vesicular
traffic; involved in Sey1p-independent homotypic ER
fusion; required for efficient nuclear fusion during
mating; forms a complex with the SNAREs Sec22p, Sec20p and
Use1p to mediate fusion of Golgi-derived vesicles at the
ER"
/codon_start=1
/product="Ufe1p"
/protein_id="NP_014718.1"
/db_xref="GeneID:854242"
/db_xref="SGD:S000005601"
/translation="MMSDLTPIFRKYVAVIDDARNEQNGIDDHVERKQEDFGNSNETC
EMFRDSFIKECARLLKFLVELNKVIKQIEKNYLDDFNMSDAEKDEFDMECRLQIQQYF
KKFEFLENYEMERHNLSLKRFQSKSHRWSKILSNKNDNTKHVIHPQDIENGVYEFRLG
VLRCLNLWIKYVSSKFTTIQQERLILENKMNFNSTPMPTLSNNADDFSADAIDISVSQ
SAPVETVQDEVKHYEETISKLTQEQLQVLETEHSELLNQKNEQLKKVETINKTILDIV
NIQNELSNHLTVQSQNINLMLNNQDDIELNIKKGNKELRKAKRAAGRTAKMTTYGAII
MGVFILFLDYVG"
gene complement(<469377..>471620)
/gene="SKI7"
/locus_tag="YOR076C"
/db_xref="GeneID:854243"
mRNA complement(<469377..>471620)
/gene="SKI7"
/locus_tag="YOR076C"
/product="Ski7p"
/transcript_id="NM_001183495.1"
/db_xref="GeneID:854243"
CDS complement(469377..471620)
/gene="SKI7"
/locus_tag="YOR076C"
/experiment="EXISTENCE:direct assay:GO:0005525 GTP binding
[PMID:26051716]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11532933]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0044877
protein-containing complex binding [PMID:11532933]"
/experiment="EXISTENCE:genetic interaction:GO:0000956
nuclear-transcribed mRNA catabolic process
[PMID:11027292]"
/experiment="EXISTENCE:genetic interaction:GO:0030163
protein catabolic process [PMID:27481473]"
/experiment="EXISTENCE:genetic interaction:GO:0070478
nuclear-transcribed mRNA catabolic process, 3'-5'
exonucleolytic nonsense-mediated decay
[PMID:12881429|PMID:12769863]"
/experiment="EXISTENCE:mutant phenotype:GO:0000956
nuclear-transcribed mRNA catabolic process
[PMID:11027292|PMID:11532933]"
/experiment="EXISTENCE:mutant phenotype:GO:0030163 protein
catabolic process [PMID:27481473]"
/experiment="EXISTENCE:mutant phenotype:GO:0030674
protein-macromolecule adaptor activity [PMID:11532933]"
/experiment="EXISTENCE:mutant phenotype:GO:0070478
nuclear-transcribed mRNA catabolic process, 3'-5'
exonucleolytic nonsense-mediated decay [PMID:12881429]"
/experiment="EXISTENCE:mutant phenotype:GO:0070481
nuclear-transcribed mRNA catabolic process, non-stop decay
[PMID:11910110|PMID:17660569]"
/experiment="EXISTENCE:mutant phenotype:GO:0070651
nonfunctional rRNA decay [PMID:19481524]"
/note="GTP-binding protein that couples the Ski complex
and exosome; putative pseudo-translational GTPase involved
in 3'-to-5' mRNA decay pathway; interacts with both the
cytoplasmic exosome and the Ski complex; eRF3-like domain
targets nonstop mRNA for degradation; null mutants have a
superkiller phenotype; SKI7 has a paralog, HBS1, that
arose from the whole genome duplication"
/codon_start=1
/product="Ski7p"
/protein_id="NP_014719.1"
/db_xref="GeneID:854243"
/db_xref="SGD:S000005602"
/translation="MSLLEQLARKRIEKSKGLLSADQSHSTSKSASLLERLHKNRETK
DNNAETKRKDLKTLLAKDKVKRSDFTPNQHSVSLSLKLSALKKSNSDLEKQGKSVTLD
SKENELPTKRKSPDDKLNLEESWKAIKEMNHYCFLKNDPCINQTDDFAFTNFIIKDKK
NSLSTSIPLSSQNSSFLSLKKHNNELLGIFVPCNLPKTTRKVAIENFNRPSPDDIIQS
AQLNAFNEKLENLNIKSVPKAEKKEPINLQTPPTESIDIHSFIATHPLNLTCLFLGDT
NAGKSTLLGHLLYDLNEISMSSMRELQKKSSNLDPSSSNSFKVILDNTKTERENGFSM
FKKVIQVENDLLPPSSTLTLIDTPGSIKYFNKETLNSILTFDPEVYVLVIDCNYDSWE
KSLDGPNNQIYEILKVISYLNKNSACKKHLIILLNKADLISWDKHRLEMIQSELNYVL
KENFQWTDAEFQFIPCSGLLGSNLNKTENITKSKYKSEFDSINYVPEWYEGPTFFSQL
YLLVEHNMNKIETTLEEPFVGTILQSSVLQPIAEINYVSLKVLINSGYIQSGQTIEIH
TQYEDFHYYGIVSRMKNSKQILETNTKNNISVGLNPDILEVLVKIHNTEDFTKKQFHI
RKGDIIIHSRKTNTLSPNLPNTLKLLALRLIKLSIQTHALSDPVDLGSELLLYHNLTH
NAVKLVKILGTNDISINPNQSLIVEVEIIEPDFALNVIDSKYITNNIVLTSIDHKVIA
VGRIACQ"
gene <471899..>472597
/gene="RTS2"
/locus_tag="YOR077W"
/db_xref="GeneID:854244"
mRNA <471899..>472597
/gene="RTS2"
/locus_tag="YOR077W"
/product="Rts2p"
/transcript_id="NM_001183496.1"
/db_xref="GeneID:854244"
CDS 471899..472597
/gene="RTS2"
/locus_tag="YOR077W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="Basic zinc-finger protein; similar to human and
mouse Kin17 proteins which are chromatin-associated
proteins involved in UV response and DNA replication"
/codon_start=1
/product="Rts2p"
/protein_id="NP_014720.1"
/db_xref="GeneID:854244"
/db_xref="SGD:S000005603"
/translation="MADYDSAKYWSKQGARRGLQKTRYYCQICQRQCKDANGFQSHNK
SPSHLRKISQVTAEDARRYNIQFEKGFLQLLKQRHGEKWIDANKVYNEYVQDRDHVHM
NATMHRSLTQFVRYLGRAGKVDVDMDIDDTSENVEGPLLIRIHPSSLSSPSEDGMLRS
QQEEQEVIAAELLKRQLNRAKRQTEKVYQPEMKSEISGDSTLKRVQVTFHGNGRVNKK
KKKVPPRKDGIKFR"
gene <472725..>473369
/gene="BUD21"
/locus_tag="YOR078W"
/gene_synonym="UTP16; YOR29-29"
/db_xref="GeneID:854245"
mRNA <472725..>473369
/gene="BUD21"
/locus_tag="YOR078W"
/gene_synonym="UTP16; YOR29-29"
/product="Bud21p"
/transcript_id="NM_001183497.1"
/db_xref="GeneID:854245"
CDS 472725..473369
/gene="BUD21"
/locus_tag="YOR078W"
/gene_synonym="UTP16; YOR29-29"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:12150911|PMID:12068309]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/experiment="EXISTENCE:direct assay:GO:0032040
small-subunit processome [PMID:12068309]"
/experiment="EXISTENCE:direct assay:GO:0034511 U3 snoRNA
binding [PMID:12068309]"
/experiment="EXISTENCE:mutant phenotype:GO:0000447
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from
5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15489292]"
/experiment="EXISTENCE:mutant phenotype:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12068309]"
/experiment="EXISTENCE:mutant phenotype:GO:0000472
endonucleolytic cleavage to generate mature 5'-end of
SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:15489292]"
/note="Component of small ribosomal subunit (SSU)
processome; interacts with the U3 snoRNA (Snr17a/b);
involved in rRNA processing and maturation; required
post-transcriptionally for efficient retrotransposition;
absence results in decreased Ty1 Gag:GFP protein levels;
originally isolated as bud-site selection mutant that
displays a random budding pattern; ortholog of human NOL7"
/codon_start=1
/product="Bud21p"
/protein_id="NP_014721.1"
/db_xref="GeneID:854245"
/db_xref="SGD:S000005604"
/translation="MSNGHVKFDADESQASASAVTDRQDDVLVISKKDKEVHSSSDEE
SDDDDAPQEEGLHSGKSEVESQITQREEAIRLEQSQLRSKRRKQNELYAKQKKSVNET
EVTDEVIAELPEELLKNIDQKDEGSTQYSSSRHVTFDKLDESDENEEALAKAIKTKKR
KTLKNLRKDSVKRGKFRVQLLSTTQDSKTLPPKKESSIIRSKDRWLNRKALNKG"
gene complement(<473475..>474416)
/gene="ATX2"
/locus_tag="YOR079C"
/gene_synonym="YOR29-30"
/db_xref="GeneID:854246"
mRNA complement(<473475..>474416)
/gene="ATX2"
/locus_tag="YOR079C"
/gene_synonym="YOR29-30"
/product="Mn(2+) transporter ATX2"
/transcript_id="NM_001183498.1"
/db_xref="GeneID:854246"
CDS complement(473475..474416)
/gene="ATX2"
/locus_tag="YOR079C"
/gene_synonym="YOR29-30"
/experiment="EXISTENCE:direct assay:GO:0000139 Golgi
membrane [PMID:8887660]"
/experiment="EXISTENCE:direct assay:GO:0005770 late
endosome [PMID:25713143]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005802 trans-Golgi
network [PMID:25713143]"
/experiment="EXISTENCE:mutant phenotype:GO:0005384
manganese ion transmembrane transporter activity
[PMID:8887660]"
/experiment="EXISTENCE:mutant phenotype:GO:0030026
intracellular manganese ion homeostasis [PMID:8887660]"
/note="Golgi membrane protein involved in manganese
homeostasis; overproduction suppresses the sod1 (copper,
zinc superoxide dismutase) null mutation"
/codon_start=1
/product="Mn(2+) transporter ATX2"
/protein_id="NP_014722.1"
/db_xref="GeneID:854246"
/db_xref="SGD:S000005605"
/translation="MKFLGVILLASFLLIATFLIGLIPLYYIDKQKSSIVTNQEGADS
ISDFTTNADTQTINDDVSSYRVKIAVLSQFGIGMLLGTSFMLVIPEGIKACVEHDGNV
GVNLLIGFLGVYVLDRLVTLWVSRKQTVYTHDAVKFQSWKDIINHPRQIWMNLIQNNV
VFALFIHGLSDGIALGTTTNNDSLLIVVLIAIVIHKIPAVLSLTSLMVSRQNLMKWEV
ICNVFLFASSTPIGYIVLSLLNLSHSPTMDWISGNLLLMSGGSLLYASFTAFVGGDSH
DHDLSVEQEVVLPHDESVYVLIGVCIPLVISYCISEE"
gene <474594..>476792
/gene="DIA2"
/locus_tag="YOR080W"
/gene_synonym="YOR29-31"
/db_xref="GeneID:854247"
mRNA <474594..>476792
/gene="DIA2"
/locus_tag="YOR080W"
/gene_synonym="YOR29-31"
/product="DNA-binding SCF ubiquitin ligase subunit DIA2"
/transcript_id="NM_001183499.1"
/db_xref="GeneID:854247"
CDS 474594..476792
/gene="DIA2"
/locus_tag="YOR080W"
/gene_synonym="YOR29-31"
/experiment="EXISTENCE:direct assay:GO:0003688 DNA
replication origin binding [PMID:16421250]"
/experiment="EXISTENCE:direct assay:GO:0004842
ubiquitin-protein transferase activity [PMID:19910927]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:19858292]"
/experiment="EXISTENCE:direct assay:GO:0019005 SCF
ubiquitin ligase complex [PMID:16421250|PMID:14747994]"
/experiment="EXISTENCE:direct assay:GO:0031146
SCF-dependent proteasomal ubiquitin-dependent protein
catabolic process [PMID:19910927]"
/experiment="EXISTENCE:direct assay:GO:0043596 nuclear
replication fork [PMID:19910927]"
/experiment="EXISTENCE:genetic interaction:GO:0001403
invasive growth in response to glucose limitation
[PMID:11063681]"
/experiment="EXISTENCE:genetic interaction:GO:0032984
protein-containing complex disassembly [PMID:26255844]"
/experiment="EXISTENCE:mutant phenotype:GO:0006275
regulation of DNA replication [PMID:16421250]"
/experiment="EXISTENCE:mutant phenotype:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:23129771]"
/experiment="EXISTENCE:mutant phenotype:GO:0016567 protein
ubiquitination [PMID:25342810|PMID:15620357]"
/experiment="EXISTENCE:mutant phenotype:GO:0030466 silent
mating-type cassette heterochromatin formation
[PMID:22844255]"
/experiment="EXISTENCE:mutant phenotype:GO:0031146
SCF-dependent proteasomal ubiquitin-dependent protein
catabolic process [PMID:19910927]"
/experiment="EXISTENCE:mutant phenotype:GO:0031509
subtelomeric heterochromatin formation [PMID:22844255]"
/experiment="EXISTENCE:mutant phenotype:GO:0032984
protein-containing complex disassembly
[PMID:25342810|PMID:26255844]"
/experiment="EXISTENCE:mutant phenotype:GO:1902979 mitotic
DNA replication termination [PMID:25342810]"
/note="Origin-binding F-box protein; forms SCF ubiquitin
ligase complex with Skp1p and Cdc53p; functions in
ubiquitination of silent chromatin structural protein
Sir4p; required to target Cdc6p for destruction during G1
phase; required for deactivation of Rad53 checkpoint
kinase, completion of DNA replication during recovery from
DNA damage, assembly of RSC complex, RSC-mediated
transcription regulation, and nucleosome positioning;
involved in invasive and pseudohyphal growth"
/codon_start=1
/product="DNA-binding SCF ubiquitin ligase subunit DIA2"
/protein_id="NP_014723.2"
/db_xref="GeneID:854247"
/db_xref="SGD:S000005606"
/translation="MSSPGNSGVAIDSTVLKAIELGTRLFKSGEYLQAKRIFTNALRV
CDSYSQEQIMRIRNAYQLDTARPDNKRLYHPRYIKILDNICACYEKLNDLKSCLDVSQ
RLLKLEPGNIKCYIRCTRTLIKLKDWKRAYKTCSRGLQLCNNDSNHLRQQKQFIKNNM
VQKQDGKRSYIDPLEETKIAKKKKNNNVLESLPKKKIKGSTKKTDLVGNLPIEILPII
FQRFTTKELVTLSLVCNKWRDKILYHLDCFQEFNLAPINFKNFVKFMDFLQQNFTRTY
RKYILSQVKVSSRITSEELRITQLLFSKMPKCINIERLILSMPTLTTTQIFKLMVRGG
TDFFTRLLELSLMITYRPDKQHELEILQTCPLLKKIELIFVNSLVPIFDGNNSVGRDG
SFNVMARHTNMQISTADNDEQGIVEEKVIYSELEKITLICDKKKIKNFPLCRALLRGQ
FPLLQKLTITGVTFPMNNQDIMNFQWLLNFPDLKELWIEDNDNCELSKFLQLLKFSNV
WKNLEKLTFRENKLYPIVNLDEDQPVTNDDEVPSMLFYKENLQNLEKLDLMGTSISGS
ALTRLCEQEYLDGRKLRSLNIGNCPNIQFPNNHAHTARMILDVNAVLKRLSKLEEINL
SHLSSLNDSTMKSFIINVPFLENLKRLDISHNFEITGISIYEFLKKFQMDHDNEAGGQ
PLAYLNIDGCSQVSHITVNMIRAQNLVTQVDCVYERDVWRKFGINSYSYS"
gene complement(<476939..>479188)
/gene="TGL5"
/locus_tag="YOR081C"
/gene_synonym="STC2"
/db_xref="GeneID:854248"
mRNA complement(<476939..>479188)
/gene="TGL5"
/locus_tag="YOR081C"
/gene_synonym="STC2"
/product="triacylglycerol lipase"
/transcript_id="NM_001183500.1"
/db_xref="GeneID:854248"
CDS complement(476939..479188)
/gene="TGL5"
/locus_tag="YOR081C"
/gene_synonym="STC2"
/EC_number="3.1.1.3"
/experiment="EXISTENCE:direct assay:GO:0004806
triacylglycerol lipase activity [PMID:16135509]"
/experiment="EXISTENCE:direct assay:GO:0005811 lipid
droplet [PMID:24868093|PMID:16135509|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006642
triglyceride mobilization [PMID:16135509]"
/experiment="EXISTENCE:direct assay:GO:0042171
lysophosphatidic acid acyltransferase activity
[PMID:20016004]"
/experiment="EXISTENCE:mutant phenotype:GO:0004806
triacylglycerol lipase activity [PMID:16135509]"
/experiment="EXISTENCE:mutant phenotype:GO:0006642
triglyceride mobilization [PMID:16135509]"
/note="Bifunctional triacylglycerol lipase and LPA
acyltransferase; lipid particle-localized triacylglycerol
(TAG) lipase involved in triacylglycerol mobilization;
catalyzes acylation of lysophosphatidic acid (LPA);
potential Cdc28p substrate; TGL5 has a paralog, TGL4, that
arose from the whole genome duplication"
/codon_start=1
/product="triacylglycerol lipase"
/protein_id="NP_014724.1"
/db_xref="GeneID:854248"
/db_xref="SGD:S000005607"
/translation="MSNTLPVTEFLLSKYYELSNTPATDSSSLFKWLYHKTLSRKQLL
ISDLSSQKKHAISYDQWNDIASRLDDLTGLSEWKTIDESSLYNYKLLQDLTIRMRHLR
TTHDYHRLLYLIRTKWVRNLGNMNNVNLYRHSHTGTKQIIHDYLEESQAVLTALIHQS
NMNDHYLLGILQQTRRNIGRTALVLSGGSTFGLFHIGVLAALFESDLMPKVISGSSAG
AIVASIFCVHTTQEIPSLLTNVLNMEFNIFNDDNSKSPNENLLIKISRFCQNGTWFNN
QPLINTMLSFLGNLTFREAYNKTGKILNITVSPASIYEQPKLLNNLTAPNVLIWSAVC
ASCSLPGVFPSTPLFEKDPHTGKIKEWGATNLHLSNMKFMDGSVDNDMPISRLSEMFN
VDHIIACQVNIHVFPLLKFSNTCVGGEIEKEITARFRNQVTKIFKFFSDETIHFLDIL
KELEFHPYLMTKLKHLFLQQYSGNVTILPDLSMVGQFHEVLKNPSQLFLLHQTTLGAR
ATWPKISMIQNNCGQEFALDKAITFLKEKIIISSSIKNPLQFYQPRFSEQIKSLSIMD
ADLPGVDLEESSSNSLSIIKSPNKTAAPGRFPLQPLPSPSSTFNKRKMDMLSPSPSPS
TSPQRSKSSFTQQGTRQKANSLSFAIGASSLRLKKSPLKVPSRPQFKKRSSYYNQNMS
AEMRKNRKKSGTISSYDVQTNSEDFPIPAIENGSFDNTLFNPSRFPMDAMSAATNDNF
MNNSDIFQN"
gene <479533..>480420
/gene="WHI5"
/locus_tag="YOR083W"
/db_xref="GeneID:854249"
mRNA <479533..>480420
/gene="WHI5"
/locus_tag="YOR083W"
/product="transcriptional repressor WHI5"
/transcript_id="NM_001183502.1"
/db_xref="GeneID:854249"
CDS 479533..480420
/gene="WHI5"
/locus_tag="YOR083W"
/experiment="EXISTENCE:direct assay:GO:0003714
transcription corepressor activity [PMID:15210111]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:24470217|PMID:14562095|PMID:15210111]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:15210111]"
/experiment="EXISTENCE:direct assay:GO:0033309 SBF
transcription complex [PMID:15210110]"
/experiment="EXISTENCE:direct assay:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:15210111]"
/experiment="EXISTENCE:genetic interaction:GO:0000082 G1/S
transition of mitotic cell cycle
[PMID:19823668|PMID:15210110|PMID:15210111]"
/experiment="EXISTENCE:genetic interaction:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:15210110|PMID:15210111]"
/experiment="EXISTENCE:genetic interaction:GO:0003714
transcription corepressor activity [PMID:19745812]"
/experiment="EXISTENCE:genetic interaction:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:19823668]"
/experiment="EXISTENCE:mutant phenotype:GO:0000082 G1/S
transition of mitotic cell cycle
[PMID:15210110|PMID:15210111]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:15210111|PMID:15210110]"
/experiment="EXISTENCE:mutant phenotype:GO:0003714
transcription corepressor activity [PMID:19745812]"
/experiment="EXISTENCE:mutant phenotype:GO:0007089
traversing start control point of mitotic cell cycle
[PMID:26390151]"
/experiment="EXISTENCE:mutant phenotype:GO:0008361
regulation of cell size [PMID:12089449]"
/experiment="EXISTENCE:mutant phenotype:GO:0010508
positive regulation of autophagy [PMID:40365021]"
/note="Repressor of G1 transcription; binds SCB binding
factor (SBF) at SCB target promoters in early G1;
phosphorylation by G1 cyclin/Cdc28p relieves repression
and promoter binding by Whi5p, drives nuclear export, and
contributes to the determination of critical cell size at
START and cell fate; dephosphorylated and re-imported into
the nucleus post-START during the starvation response,
reversing commitment; repressed in M/early G phase;
autophagy regulator and Atg1p substrate; functional Rb
homolog"
/codon_start=1
/product="transcriptional repressor WHI5"
/protein_id="NP_014726.1"
/db_xref="GeneID:854249"
/db_xref="SGD:S000005609"
/translation="MSLRTPKRSRTSDEQEQEQEQEQVQNPDTHVNNEHQQRPGPTTL
LSTPVRLKNGFGTPSPPSPPGITKSITKSRRRPSTTSLQGIFMSPVNKRRVGITAHGR
VYDHNDDGHESESEDDENEEENENQKKYDGHVSMPLLPPTTPKSRRSEVFLSPSPRLR
SPPTAARRSTGERPIREISHTLRTRLNYALVKLQNGWTDKTLPELETELAPAVQTPPR
RYHNRFPDSADAGTSAHTAFLQALGGHPPREEATAVETLMLLSSPTKKQQHRPVPATS
AGEPTDETEPESDTEVETS"
gene <480587..>481750
/gene="LPX1"
/locus_tag="YOR084W"
/db_xref="GeneID:854251"
mRNA <480587..>481750
/gene="LPX1"
/locus_tag="YOR084W"
/product="triglyceride lipase"
/transcript_id="NM_001183503.1"
/db_xref="GeneID:854251"
CDS 480587..481750
/gene="LPX1"
/locus_tag="YOR084W"
/experiment="EXISTENCE:direct assay:GO:0004806
triacylglycerol lipase activity [PMID:24187129]"
/experiment="EXISTENCE:direct assay:GO:0005782 peroxisomal
matrix [PMID:12135984]"
/experiment="EXISTENCE:genetic interaction:GO:0019433
triglyceride catabolic process [PMID:24187129]"
/note="Peroxisomal matrix-localized lipase; required for
normal peroxisome morphology; contains a peroxisomal
targeting signal type 1 (PTS1) and a lipase motif;
peroxisomal import requires the PTS1 receptor, Pex5p and
self-interaction; transcriptionally activated by Yrm1p
along with genes involved in multidrug resistance; oleic
acid inducible"
/codon_start=1
/product="triglyceride lipase"
/protein_id="NP_014727.1"
/db_xref="GeneID:854251"
/db_xref="SGD:S000005610"
/translation="MEQNRFKKETKTCSASWPRAPQSTLCATDRLELTYDVYTSAERQ
RRSRTATRLNLVFLHGSGMSKVVWEYYLPRLVAADAEGNYAIDKVLLIDQVNHGDSAV
RNRGRLGTNFNWIDGARDVLKIATCELGSIDSHPALNVVIGHSMGGFQALACDVLQPN
LFHLLILIEPVVITRKAIGAGRPGLPPDSPQIPENLYNSLRLKTCDHFANESEYVKYM
RNGSFFTNAHSQILQNIIDFERTKASGDDEDGGPVRTKMEQAQNLLCYMNMQTFAPFL
ISNVKFVRKRTIHIVGARSNWCPPQNQLFLQKTLQNYHLDVIPGGSHLVNVEAPDLVI
ERINHHIHEFVLTSPLQSSHIPQLTLEERAVMFDRAFDSFKNEALVKTTKQKL"
gene <482033..>483085
/gene="OST3"
/locus_tag="YOR085W"
/db_xref="GeneID:854252"
mRNA <482033..>483085
/gene="OST3"
/locus_tag="YOR085W"
/product="dolichyl-diphosphooligosaccharide--protein
glycotransferase OST3"
/transcript_id="NM_001183504.1"
/db_xref="GeneID:854252"
CDS 482033..483085
/gene="OST3"
/locus_tag="YOR085W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0008250
oligosaccharyltransferase complex [PMID:10358084]"
/experiment="EXISTENCE:direct assay:GO:0015035
protein-disulfide reductase activity [PMID:23416356]"
/experiment="EXISTENCE:genetic interaction:GO:0006487
protein N-linked glycosylation [PMID:10358084]"
/experiment="EXISTENCE:genetic interaction:GO:0035269
protein O-linked glycosylation via mannose
[PMID:22492205]"
/experiment="EXISTENCE:mutant phenotype:GO:0004579
dolichyl-diphosphooligosaccharide-protein glycotransferase
activity [PMID:7622558]"
/experiment="EXISTENCE:mutant phenotype:GO:0006487 protein
N-linked glycosylation [PMID:28598353]"
/experiment="EXISTENCE:mutant phenotype:GO:0009101
glycoprotein biosynthetic process [PMID:7622558]"
/experiment="EXISTENCE:mutant phenotype:GO:0016020
membrane [PMID:7622558]"
/experiment="EXISTENCE:physical interaction:GO:0006487
protein N-linked glycosylation [PMID:9405463]"
/experiment="EXISTENCE:physical interaction:GO:0008250
oligosaccharyltransferase complex [PMID:9405463]"
/note="Gamma subunit of the oligosaccharyltransferase
complex of the ER lumen; complex catalyzes
asparagine-linked glycosylation of newly synthesized
proteins; Ost3p is important for N-glycosylation of a
subset of proteins including modification of Pmt2p at N131
and N403"
/codon_start=1
/product="dolichyl-diphosphooligosaccharide--protein
glycotransferase OST3"
/protein_id="NP_014728.1"
/db_xref="GeneID:854252"
/db_xref="SGD:S000005611"
/translation="MNWLFLVSLVFFCGVSTHPALAMSSNRLLKLANKSPKKIIPLKD
SSFENILAPPHENAYIVALFTATAPEIGCSLCLELESEYDTIVASWFDDHPDAKSSNS
DTSIFFTKVNLEDPSKTIPKAFQFFQLNNVPRLFIFKPNSPSILDHSVISISTDTGSE
RMKQIIQAIKQFSQVNDFSLHLPMDWTPIITSTIITFITVLLFKKQSKLMFSIISSRI
IWATLSTFFIICMISAYMFNQIRNTQLAGVGPKGEVMYFLPNEFQHQFAIETQVMVLI
YGTLAALVVVLVKGIQFLRSHLYPETKKAYFIDAILASFCALFIYVFFAALTTVFTIK
SPAYPFPLLRLSAPFK"
gene complement(<483219..>486779)
/gene="TCB1"
/locus_tag="YOR086C"
/db_xref="GeneID:854253"
mRNA complement(<483219..>486779)
/gene="TCB1"
/locus_tag="YOR086C"
/product="tricalbin"
/transcript_id="NM_001183505.1"
/db_xref="GeneID:854253"
CDS complement(483219..486779)
/gene="TCB1"
/locus_tag="YOR086C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008289 lipid
binding [PMID:15049706]"
/experiment="EXISTENCE:direct assay:GO:0032541 cortical
endoplasmic reticulum [PMID:22250200]"
/experiment="EXISTENCE:direct assay:GO:0071561
nucleus-vacuole junction [PMID:41132095]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0055091
phospholipid homeostasis [PMID:35440494]"
/experiment="EXISTENCE:genetic interaction:GO:0060304
regulation of phosphatidylinositol dephosphorylation
[PMID:23237950]"
/experiment="EXISTENCE:genetic interaction:GO:0090158
endoplasmic reticulum membrane organization
[PMID:23237950]"
/experiment="EXISTENCE:mutant phenotype:GO:0035621 ER to
Golgi ceramide transport [PMID:33205016]"
/note="Lipid-binding tricalbin; one of at least 7 proteins
(Ice2p, Ist2p, Scs2/Scs22p, Tcb1-Tcb3p) that affect cER
tethering and contact with the plasma membrane (PM);
regulates PI4P levels by controlling access of the
phosphatase Sac1p to its PM substrate PI4P; regulates PM
phospholipid homeostasis; contains 3 calcium and
lipid-binding domains; localizes to the cortical ER and
mitochondria; localizes to the nucleus-vacuole junction
during glucose restriction; C-termini of Tcb2p and Tcb3p
interact"
/codon_start=1
/product="tricalbin"
/protein_id="NP_014729.1"
/db_xref="GeneID:854253"
/db_xref="SGD:S000005612"
/translation="MAKEDTGVTAPKKPETAQVANINGIDKLEPPKTKEETESSKSVS
SEKAAHASDESFKRSIHEASYVGWKQIGGWEDKDELTLDDELMDMTRETFLDNIIPDS
LYGDWYHSVAIFFIGGVASFALGHYKFSMGSAFFVIVITSLLYRTSAKKYRGSIRELV
QKEFTVQKVENDYESLEWLNAFLDKYWPILEPSVSQLIVQQANEQMATNEAIPKFITQ
LWIDELTLGVKPPRVDLVKTFQNTASDVVVMDWGISFTPHDLCDMSAKQVRNYVNELA
VVKAKIFGITIPVSVSDIAFKAHARVKFKLMTPFPHVETVNIQLLKVPDFDFVATLFG
RSIFNWEILAIPGLMTLIQKMAKKYMGPILLPPFSLQLNIPQLLSGSNLSIGILEITV
KNAKGLKRTSSILNESIDPYLSFEFNDISIAKTRTVRDTLNPVWDETLYVLLNSFTDP
LTISVYDKRAKLKDKVLGRIQYNLNTLHDKTTQRNLKAQFLRNSKPVGELTFDLRFFP
TLEEKKLPDGSVEELPDLNTGIAKVVVEEGSRFAEEEQKVTAYVEVYLNAKLVLTTGK
ATDTGTLKWNSDYEAVIADRRKTRYKFVVKDGKGEEIGSTIQTLNDLIDRSQVNKNLI
PLKNQKGDIKITTYWRPVRLEIGSNSVAYTPPIGAIRVFIEKANDLRNLEKFGTIDPY
CKVLVNGLSKGRTDFKSQTLNPVWNQVIYVAVTSPNQRITLQCMDVETVNKDRSLGEF
NVNVQDLFKKDENDKYEETIDEKAKVGRLVMPKKKPKGTITYYTSFYPALPVLTLEEI
QDLDKVNKKKKALELRKSAIDEKKISKEDKAKFDQEWNEVKELEDMYSNRQKLDLPEL
LQYNQGVLAVTVLNGELPDSGLYVQAFFDDNGHPRFVSPRIPSRIVKNGWSGDVIIKE
LDKSITTFRVAKNKNYNRVEKCVCEVELPTQELVKNCYYKPSILHLSGEGSAKLMLQI
SWFPIDTKQLPANDLITNSGDLTIMSRSAENLIASDLNGYSDPYLKYYINNEEDCAYK
TKVVKKTLNPKWNDEGTIQINNRLNDVLRIKVMDWDSTSADDTIGTAEIPLNKVKVEG
TTELDVPVEGLENAGQDGGMLHLAFSFKPRYTISVSKREKKVGDIASKGLGTGLKAGT
TVIGGGVGAIGKIKKGVFGGLGSLTNHKKNHEMGEEETKF"
gene 487439..487512
/locus_tag="YNCO0018W"
/db_xref="GeneID:854254"
tRNA 487439..487512
/locus_tag="YNCO0018W"
/product="tRNA-Asn"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Asparagine tRNA (tRNA-Asn), predicted by
tRNAscan-SE analysis"
/db_xref="GeneID:854254"
/db_xref="SGD:S000006676"
gene <487707..>489734
/gene="YVC1"
/locus_tag="YOR087W"
/gene_synonym="YOR088W"
/db_xref="GeneID:854255"
mRNA <487707..>489734
/gene="YVC1"
/locus_tag="YOR087W"
/gene_synonym="YOR088W"
/product="Yvc1p"
/transcript_id="NM_001183506.1"
/db_xref="GeneID:854255"
CDS 487707..489734
/gene="YVC1"
/locus_tag="YOR087W"
/gene_synonym="YOR088W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:11427713|PMID:11781332]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:20035756|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005227
calcium-activated cation channel activity [PMID:1700419]"
/experiment="EXISTENCE:direct assay:GO:0005244
voltage-gated monoatomic ion channel activity
[PMID:1700419]"
/experiment="EXISTENCE:direct assay:GO:0005262 calcium
channel activity [PMID:11781332]"
/experiment="EXISTENCE:direct assay:GO:0005267 potassium
channel activity [PMID:1700419]"
/experiment="EXISTENCE:direct assay:GO:0005272 sodium
channel activity [PMID:1700419]"
/experiment="EXISTENCE:direct assay:GO:0030003
intracellular monoatomic cation homeostasis
[PMID:11781332|PMID:11427713]"
/experiment="EXISTENCE:direct assay:GO:1990816
vacuole-mitochondrion membrane contact site
[PMID:25026036]"
/experiment="EXISTENCE:mutant phenotype:GO:0030003
intracellular monoatomic cation homeostasis
[PMID:11781332|PMID:11427713]"
/experiment="EXISTENCE:mutant phenotype:GO:0097553 calcium
ion transmembrane import into cytosol [PMID:27708136]"
/note="Vacuolar cation channel; mediates release of Ca(2+)
from the vacuole in response to hyperosmotic shock"
/codon_start=1
/product="Yvc1p"
/protein_id="NP_014730.2"
/db_xref="GeneID:854255"
/db_xref="SGD:S000005613"
/translation="MVSANGDLHLPISNEQCMPENNGSLGFEAPTPRQILRVTLNLKY
LIDKVVPIVYDPNDIVCDHSEILSPKVVKLAYEACGGNPKDKANKRKYQSVIIFSLLK
VCEWYSILATMEVHNAKLYETRNLASQQLCKLLIEREETRDLQFLFMQLLLRRYVINE
NDEDQEPLNALELATDMHCTTVIGSSGFQRCLKWIWRGWIVQNGLDPTTFIKDDSLAE
VSLISHFNPVRLKAPVYQNYLQMIFSFLFLGLYTLVVNGKDSERVQSFDLLESIFYVF
NTGFILDELTKLYYIGYAHLSFWNLFNDTTYLIITFAMGFRAMSVTPLNAKYSSEDWD
KISYRVLSCAAPFVWSRLLLYLESQRFIGIMLVILKHMMKESIVFFFLLFLIMIGFTQ
GFLGLDSADGKRDITGPILGNLTITVLGLGSFDVFEEFAPPYAAILYYGYYFIVSVIL
LNILIALYSTAYQKVIDNADDEYMALMSQKTLRYIRAPDEDVYVSPLNLIEVFMTPIF
RILPPKRAKDLSYTVMTIVYSPFLLLISVKETREARRIKYNRMKRLNDDANEYDTPWD
LTDGYLDDDDGLFSDNRNSGMRATQLKNSRSLKLQRTAEQEDVHFKVPKKWYKNVKKC
SPSFEQYDNDDTEDDAGEDKDEVKELTKKVENLTAVITDLLEKLDIKDKKE"
rep_origin 489683..489942
/note="ARS1501; Autonomously Replicating Sequence"
/db_xref="SGD:S000007554"
gene complement(<490196..>490828)
/gene="VPS21"
/locus_tag="YOR089C"
/gene_synonym="VPS12; VPT12; YPT21; YPT51"
/db_xref="GeneID:854256"
mRNA complement(<490196..>490828)
/gene="VPS21"
/locus_tag="YOR089C"
/gene_synonym="VPS12; VPT12; YPT21; YPT51"
/product="Rab family GTPase VPS21"
/transcript_id="NM_001183508.1"
/db_xref="GeneID:854256"
CDS complement(490196..490828)
/gene="VPS21"
/locus_tag="YOR089C"
/gene_synonym="VPS12; VPT12; YPT21; YPT51"
/experiment="EXISTENCE:direct assay:GO:0003924 GTPase
activity [PMID:11210571]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:16407407]"
/experiment="EXISTENCE:direct assay:GO:0005769 early
endosome [PMID:31754649]"
/experiment="EXISTENCE:genetic interaction:GO:0006895
Golgi to endosome transport [PMID:22748138|PMID:23612966]"
/experiment="EXISTENCE:genetic interaction:GO:0032511 late
endosome to vacuole transport via multivesicular body
sorting pathway [PMID:22748138]"
/experiment="EXISTENCE:genetic interaction:GO:0036010
protein localization to endosome [PMID:23264632]"
/experiment="EXISTENCE:genetic interaction:GO:0036258
multivesicular body assembly [PMID:22748138]"
/experiment="EXISTENCE:mutant phenotype:GO:0000011 vacuole
inheritance [PMID:1493335]"
/experiment="EXISTENCE:mutant phenotype:GO:0003924 GTPase
activity [PMID:8163546]"
/experiment="EXISTENCE:mutant phenotype:GO:0005770 late
endosome [PMID:11872141]"
/experiment="EXISTENCE:mutant phenotype:GO:0006623 protein
targeting to vacuole [PMID:8163546]"
/experiment="EXISTENCE:mutant phenotype:GO:0006895 Golgi
to endosome transport [PMID:23612966|PMID:22748138]"
/experiment="EXISTENCE:mutant phenotype:GO:0006897
endocytosis [PMID:8163546]"
/experiment="EXISTENCE:mutant phenotype:GO:0061709
reticulophagy [PMID:26181331]"
/note="Endosomal Rab family GTPase; required for endocytic
transport and sorting of vacuolar hydrolases; required for
endosomal localization of the CORVET complex; required
with YPT52 for MVB biogenesis and sorting; involved in
autophagy and ionic stress tolerance; geranylgeranylation
required for membrane association; protein abundance
increases in response to DNA replication stress; mammalian
Rab5 homolog; VPS21 has a paralog, YPT53, that arose from
the whole genome duplication"
/codon_start=1
/product="Rab family GTPase VPS21"
/protein_id="NP_014732.1"
/db_xref="GeneID:854256"
/db_xref="SGD:S000005615"
/translation="MNTSVTSIKLVLLGEAAVGKSSIVLRFVSNDFAENKEPTIGAAF
LTQRVTINEHTVKFEIWDTAGQERFASLAPMYYRNAQAALVVYDVTKPQSFIKARHWV
KELHEQASKDIIIALVGNKIDMLQEGGERKVAREEGEKLAEEKGLLFFETSAKTGENV
NDVFLGIGEKIPLKTAEEQNSASNERESNNQRVDLNAANDGTSANSACSC"
gene complement(<491123..>492841)
/gene="PTC5"
/locus_tag="YOR090C"
/gene_synonym="PPP1"
/db_xref="GeneID:854257"
mRNA complement(<491123..>492841)
/gene="PTC5"
/locus_tag="YOR090C"
/gene_synonym="PPP1"
/product="type 2C protein phosphatase PTC5"
/transcript_id="NM_001183509.1"
/db_xref="GeneID:854257"
CDS complement(491123..492841)
/gene="PTC5"
/locus_tag="YOR090C"
/gene_synonym="PPP1"
/EC_number="3.1.3.43"
/experiment="EXISTENCE:direct assay:GO:0004722 protein
serine/threonine phosphatase activity [PMID:10580002]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:16643908|PMID:24769239|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005758
mitochondrial intermembrane space [PMID:22984289]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:32398688]"
/experiment="EXISTENCE:mutant phenotype:GO:0004741
[pyruvate dehydrogenase (acetyl-transferring)]-phosphatase
activity [PMID:18180296]"
/experiment="EXISTENCE:mutant phenotype:GO:0070262
peptidyl-serine dephosphorylation [PMID:18180296]"
/note="Mitochondrial type 2C protein phosphatase (PP2C);
involved in regulation of pyruvate dehydrogenase activity
by dephosphorylating the serine 133 of the Pda1p subunit;
localizes to the intermembrane space and is imported via
the presequence pathway and processed by the inner
membrane protease (Imp1p-Imp2p); acts in concert with
kinases Pkp1p and Pkp2p and phosphatase Ptc6p"
/codon_start=1
/product="type 2C protein phosphatase PTC5"
/protein_id="NP_014733.1"
/db_xref="GeneID:854257"
/db_xref="SGD:S000005616"
/translation="MSPLTRTVAIKKTVKVLSKCQSGREYTQKFLQRAYSTSHANSTY
YSRTKLFISSHSKALNIALLSGSLLLTYSYYSPKKILSLDTINGIKDYSTNTSGNINM
PSPNPKGTETQKSQRSQNDQSVLILNDSKIEAKLHDREESHFVNRGTGIFRYDVAQLP
SNHPIEDDHVEQIITIPIESEDGKSIEKDLYFFGIFDGHGGPFTSEKLSKDLVRYVAY
QLGQVYDQNKTVFHSDPNQLIDSAISKGFLKLDNDLVIESFRKLFQDPNNTNIANTLP
AISGSCALLSLYNSTNSILKVAVTGDSRALICGLDNEGNWTVKSLSTDQTGDNLDEVR
RIRKEHPGEPNVIRNGRILGSLQPSRAFGDYRYKIKEVDGKPLSDLPEVAKLYFRREP
RDFKTPPYVTAEPVITSAKIGENTKFMVMGSDGLFELLTNEEIASLVIRWMDKNMNLA
PVKAEPGKLPKVIDVSEDKEAQRPAFRYKDNNSSSPSGSNPEYLIEDKNVATHLIRNA
LSAGGRKEYVSALVSIPSPMSRRYRDDLTVTVAFFGDSGTPSIVSNATSIVMNPEATT
KPKPRL"
gene <493433..>494470
/gene="TMA46"
/locus_tag="YOR091W"
/db_xref="GeneID:854258"
mRNA <493433..>494470
/gene="TMA46"
/locus_tag="YOR091W"
/product="translation machinery-associated protein TMA46"
/transcript_id="NM_001183510.1"
/db_xref="GeneID:854258"
CDS 493433..494470
/gene="TMA46"
/locus_tag="YOR091W"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:10684247]"
/experiment="EXISTENCE:genetic interaction:GO:0002181
cytoplasmic translation [PMID:21076151]"
/note="hypothetical protein that associates with
translating ribosomes; interacts with GTPase Rbg1p"
/codon_start=1
/product="translation machinery-associated protein TMA46"
/protein_id="NP_014734.2"
/db_xref="GeneID:854258"
/db_xref="SGD:S000005617"
/translation="MPPKKGKQAQAAGKKKDNVDKTFGMKNKNRSTKVQKYIKQVQSQ
SDPKKEEMRLKKLEEKKRREAEEAERRALFNPVADQRVRAGVDPKSMVCALFKLGNCN
KGAKCKFSHDLNVGRRMEKKDLYQDTRSEKENDTMDNWDEEKLRKVILSKHGNPKTTT
DKVCKYFIEAVENGKYGWFWICPNGGDKCMYRHSLPEGFVLKTNEQKRLERESLEKQP
KITLEEFIETERGKLDKSKLTPITIANFAQWKKDHVIAKINAEKKLSSKRKPTGREII
LKMSAENKSFETDNADMPDDVTQGSAWDLTEFTDALKKADHQDDGGIKDYGDGSNPTF
DIKKANSATLA"
gene <495127..>496968
/gene="ECM3"
/locus_tag="YOR092W"
/gene_synonym="YOR3165W"
/db_xref="GeneID:854259"
mRNA <495127..>496968
/gene="ECM3"
/locus_tag="YOR092W"
/gene_synonym="YOR3165W"
/product="putative ATPase ECM3"
/transcript_id="NM_001183511.1"
/db_xref="GeneID:854259"
CDS 495127..496968
/gene="ECM3"
/locus_tag="YOR092W"
/gene_synonym="YOR3165W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:22842922|PMID:14562095|PMID:26928762]"
/note="Non-essential hypothetical protein; involved in
signal transduction and the genotoxic response; induced
rapidly in response to treatment with 8-methoxypsoralen
and UVA irradiation; relocalizes from ER to cytoplasm upon
DNA replication stress; ECM3 has a paralog, YNL095C, that
arose from the whole genome duplication"
/codon_start=1
/product="putative ATPase ECM3"
/protein_id="NP_014735.1"
/db_xref="GeneID:854259"
/db_xref="SGD:S000005618"
/translation="MTHITLGQAIWASVRPIIKIYLIIGVGFGLCKMNILTVQATRSI
SDIVLTILLPCLSFNKIVANIEDNDIKDVGIICLTSVILFATGLGFAFIVRSVLPVPK
RWRGGILAGGMFPNISDLPIAYLQSMDQGFIFTEAEGEKGVANVIIFLAMFLICVFNL
GGFRLIENDFHYKGDDDEENTLTNDDSAQQPTQPIEGNSSSSSNQDILKEPNESTVPN
SSQASYISEKNKKEKTELSVPKPTHTAPPAIDDRSSNSSAVVSIDSITHSLRTNHVDA
QSVSELNDPTYRTRSQPIAYTTESRTSHVHNNRRNSITGSLRSIDMRELPAEGMSDLI
REYSNVDQYGRRRKSSISSQGAPSVLQADGTISPNLTRTSTLQRVKTSNLTRIITSDA
TVSKKDIETSGSSLPKWLQKFPLTKFFVFFLKNCLRPCSMAVILALIIAFIPWVKALF
VTTSNTPKIKQAPDNAPALTFIMDFTSYVGAASVPFGLILLGATLGRLKIGKLYPGFW
KSAVVLVFLRQCIMPIFGVLWCDRLVKAGWLNWENDKMLLFVTAITWNLPTMTTLIYF
TASYTPEDETEPVQMECTSFFLMLQYPLMVVSLPFLVSYFIKVQMKL"
gene complement(<497506..>502452)
/gene="CMR2"
/locus_tag="YOR093C"
/gene_synonym="DIP2"
/db_xref="GeneID:854260"
mRNA complement(<497506..>502452)
/gene="CMR2"
/locus_tag="YOR093C"
/gene_synonym="DIP2"
/product="Cmr2p"
/transcript_id="NM_001183512.1"
/db_xref="GeneID:854260"
CDS complement(497506..502452)
/gene="CMR2"
/locus_tag="YOR093C"
/gene_synonym="DIP2"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:1990816
vacuole-mitochondrion membrane contact site
[PMID:35766356]"
/experiment="EXISTENCE:mutant phenotype:GO:0006970
response to osmotic stress [PMID:35766356]"
/experiment="EXISTENCE:mutant phenotype:GO:0046339
diacylglycerol metabolic process [PMID:35766356]"
/experiment="EXISTENCE:mutant phenotype:GO:0097576 vacuole
fusion [PMID:35766356]"
/note="Protein involved in regulation of diacylglycerol
lipids levels; deletion causes sensitivity to unfolded
protein response-inducing agents"
/codon_start=1
/product="Cmr2p"
/protein_id="NP_014736.1"
/db_xref="GeneID:854260"
/db_xref="SGD:S000005619"
/translation="MDFSIPPTLPLDLQSRLNELIQDYKDENLTRKGYETKRKQLLDK
FEISQMRPYTPLRSPNSRKSKHLHRRNTSLASSITSLPNSIDRRHSIYRVTTINSTSA
NNTPRRRSKRYTASLQSSLPGSSDENGSVKDAVYNPMIPLLPRHTGAENTSSGDSAMT
DSLPLILRGRFEHYDGQTAMISINSKGKETFITWDKLYLKAERVAHELNKSHLYKMDK
ILLWYNKNDVIEFTIALLGCFISGMAAVPVSFETYSLREILEIIKVTNSKFVLISNAC
HRQLDNLYSSSNHSKVKLVKNDVFQQIKFVKTDDLGTYTKAKKTSPTFDIPNISYIEF
TRTPLGRLSGVVMKHNILINQFETMTKILNSRSMPHWKQKSQSIRKPFHKKIMATNSR
FVILNSLDPTRSTGLIMGVLFNLFTGNLMISIDSSILQRPGGYENIIDKFRADILLND
QLQLKQVVINYLENPESAFSKKHKIDFSCIKSCLTSCTTIDTDVSEMVVHKWLKNLGC
IDAPFCYSPMLTLLDFGGIFISIRDQLGNLENFPIHNSKLRLQNELFINREKLKLNEV
ECSITAMINSSSSFKDYLKLETFGFPIPDITLCVVNPDTNTLVQDLTVGEIWISSNHI
TDEFYQMDKVNEFVFKAKLNYSEMFSWAKYEMPTNEKSQAVTEQLDTILNICPANTYF
MRTKLMGFVHNGKIYVLSLIEDMFLQNRLIRLPNWAHTSNLLYAKKGNQSAQPKGNTG
AESTKAIDISSLSGETSSGYKRVVESHYLQQITETVVRTVNTVFEVAAFELQHHKEEH
FLVMVVESSLAKTEEESKNGETTDTTLMKFAETQRNKLETKMNDLTDQIFRILWIFHK
IQPMCILVVPRDTLPRRYCSLELANSTVEKKFLNNDLSAQFVKFQFDNVILDFLPHSA
YYNESILSEHLSKLRKMALQEEYAMIEPAYRNGGPVKPKLALQCSGVDYRDESVDTRS
HTKLTDFKSILEILEWRISNYGNETAFSDGTNTNLVNSSASNDNNVHKKVSWASFGKI
VAGFLKKIVGSKIPLKHGDPIIIMCENSVEYVAMIMACLYCNLLVIPLPSVKESVIEE
DLKGLVNIIQSYKVKRVFVDAKLHSLLNDNNVVNKCFKKYKSLIPKITVFSKVKTKNA
LTVSMFKNVLKQKFGAKPGTRIGMTPCVVWVNTEYDVTSNIHVTMTHSSLLNASKIVK
ETLQLRNNSPLFSICSHTSGLGFMFSCLLGIYTGASTCLFSLTDVLTDPKEFLIGLQN
LNVKDLYLKLETLYALLDRASSLIEGFKNKKENINSAKNNTSGSLREDVFKGVRNIMI
PFPNRPRIYTIENILKRYSTISLSCTQISYVYQHHFNPLISLRSYLDIPPVDLYLDPF
SLREGIIREVNPNDVSAGNYIKIQDSGVVPVCTDVSVVNPETLLPCVDGEFGEIWCCS
EANAFDYFVCNSSKNKLYKDPFITEQFKSKMKSEVNNTLSYLRTGDLGFIKNVSCTNS
QGEVVNLNLLFVLGSIHESIEILGLTHFVSDLERTVKDVHSDIGSCLIAKAGGLLVCL
IRCKERHNPILGNLTTLIVSELLNKHGVILDLCTFVRTKGISPKNSSMIMEVWAKNRA
SIMQAWFDQKIQIEAQFGINYGENISIYLLSDYEKDNI"
gene <502795..>503346
/gene="ARF3"
/locus_tag="YOR094W"
/gene_synonym="ARL2"
/db_xref="GeneID:854261"
mRNA <502795..>503346
/gene="ARF3"
/locus_tag="YOR094W"
/gene_synonym="ARL2"
/product="Arf family GTPase ARF3"
/transcript_id="NM_001183513.1"
/db_xref="GeneID:854261"
CDS 502795..503346
/gene="ARF3"
/locus_tag="YOR094W"
/gene_synonym="ARL2"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:20844764]"
/experiment="EXISTENCE:direct assay:GO:0003924 GTPase
activity [PMID:8063710]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:17786213]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:17786213]"
/experiment="EXISTENCE:genetic interaction:GO:0006897
endocytosis [PMID:18208507]"
/experiment="EXISTENCE:genetic interaction:GO:0051017
actin filament bundle assembly [PMID:17425670]"
/experiment="EXISTENCE:genetic interaction:GO:0051666
actin cortical patch localization
[PMID:17425670|PMID:14668359]"
/experiment="EXISTENCE:mutant phenotype:GO:0000282
cellular bud site selection [PMID:12972567]"
/experiment="EXISTENCE:mutant phenotype:GO:0001403
invasive growth in response to glucose limitation
[PMID:23783029]"
/experiment="EXISTENCE:mutant phenotype:GO:0006886
intracellular protein transport [PMID:8063710]"
/experiment="EXISTENCE:mutant phenotype:GO:0006897
endocytosis [PMID:18208507]"
/experiment="EXISTENCE:mutant phenotype:GO:0008047 enzyme
activator activity [PMID:23783029]"
/experiment="EXISTENCE:mutant phenotype:GO:0010513
positive regulation of phosphatidylinositol biosynthetic
process [PMID:18208507]"
/experiment="EXISTENCE:mutant phenotype:GO:0036267
invasive filamentous growth [PMID:23783029]"
/experiment="EXISTENCE:mutant phenotype:GO:0051666 actin
cortical patch localization [PMID:18397879]"
/note="Glucose-repressible ADP-ribosylation factor; GTPase
of Ras superfamily involved in regulating cell polarity
and invasive growth; localizes to dynamic spots at plasma
membrane and modulates PtdIns(4,5)P2 levels to facilitate
endocytosis; required for localization of endocytic
protein Lsb5p to correct cortical site in cells; also has
mRNA binding activity; homolog of mammalian Arf6"
/codon_start=1
/product="Arf family GTPase ARF3"
/protein_id="NP_014737.1"
/db_xref="GeneID:854261"
/db_xref="SGD:S000005620"
/translation="MGNSISKVLGKLFGSKEMKILMLGLDKAGKTTILYKLKLNKIKT
STPTVGFNVETVTYKNVKFNMWDVGGQQRLRPLWRHYFPATTALIFVIDSSARNRMEE
AKEELYSIIGEKEMENVVLLVWANKQDLKDAMKPQEVSDFLELEKNLKNQPWCVIGSN
ALSGQGLVEGLSWISNNTNVPKK"
gene complement(<503552..>504328)
/gene="RKI1"
/locus_tag="YOR095C"
/db_xref="GeneID:854262"
mRNA complement(<503552..>504328)
/gene="RKI1"
/locus_tag="YOR095C"
/product="ribose-5-phosphate isomerase RKI1"
/transcript_id="NM_001183514.1"
/db_xref="GeneID:854262"
CDS complement(503552..504328)
/gene="RKI1"
/locus_tag="YOR095C"
/EC_number="5.3.1.6"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0004751
ribose-5-phosphate isomerase activity [PMID:8929392]"
/experiment="EXISTENCE:mutant phenotype:GO:0006098
pentose-phosphate shunt [PMID:8929392]"
/experiment="EXISTENCE:mutant phenotype:GO:0008615
pyridoxine biosynthetic process [PMID:14690456]"
/note="Ribose-5-phosphate ketol-isomerase; catalyzes the
interconversion of ribose 5-phosphate and ribulose
5-phosphate in the pentose phosphate pathway; participates
in pyridoxine biosynthesis"
/codon_start=1
/product="ribose-5-phosphate isomerase RKI1"
/protein_id="NP_014738.1"
/db_xref="GeneID:854262"
/db_xref="SGD:S000005621"
/translation="MAAGVPKIDALESLGNPLEDAKRAAAYRAVDENLKFDDHKIIGI
GSGSTVVYVAERIGQYLHDPKFYEVASKFICIPTGFQSRNLILDNKLQLGSIEQYPRI
DIAFDGADEVDENLQLIKGGGACLFQEKLVSTSAKTFIVVADSRKKSPKHLGKNWRQG
VPIEIVPSSYVRVKNDLLEQLHAEKVDIRQGGSAKAGPVVTDNNNFIIDADFGEISDP
RKLHREIKLLVGVVETGLFIDNASKAYFGNSDGSVEVTEK"
gene <505794..>506767
/gene="RPS7A"
/locus_tag="YOR096W"
/gene_synonym="RPS30"
/db_xref="GeneID:854263"
mRNA join(<505794..505937,506339..>506767)
/gene="RPS7A"
/locus_tag="YOR096W"
/gene_synonym="RPS30"
/product="40S ribosomal protein eS7 RPS7A"
/transcript_id="NM_001183515.1"
/db_xref="GeneID:854263"
CDS join(505794..505937,506339..506767)
/gene="RPS7A"
/locus_tag="YOR096W"
/gene_synonym="RPS30"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/experiment="EXISTENCE:direct assay:GO:0032040
small-subunit processome [PMID:15590835]"
/experiment="EXISTENCE:genetic interaction:GO:0042254
ribosome biogenesis [PMID:16246728]"
/note="Protein component of the small (40S) ribosomal
subunit; interacts with Kti11p; deletion causes
hypersensitivity to zymocin; homologous to mammalian
ribosomal protein S7, no bacterial homolog; RPS7A has a
paralog, RPS7B, that arose from the whole genome
duplication"
/codon_start=1
/product="40S ribosomal protein eS7 RPS7A"
/protein_id="NP_014739.1"
/db_xref="GeneID:854263"
/db_xref="SGD:S000005622"
/translation="MSAPQAKILSQAPTELELQVAQAFVELENSSPELKAELRPLQFK
SIREIDVAGGKKALAIFVPVPSLAGFHKVQTKLTRELEKKFQDRHVIFLAERRILPKP
SRTSRQVQKRPRSRTLTAVHDKILEDLVFPTEIVGKRVRYLVGGNKIQKVLLDSKDVQ
QIDYKLESFQAVYNKLTGKQIVFEIPSETH"
gene complement(<506978..>507505)
/locus_tag="YOR097C"
/db_xref="GeneID:854264"
mRNA complement(<506978..>507505)
/locus_tag="YOR097C"
/product="uncharacterized protein"
/transcript_id="NM_001183516.1"
/db_xref="GeneID:854264"
CDS complement(506978..507505)
/locus_tag="YOR097C"
/note="hypothetical protein; identified as interacting
with Hsp82p in a high-throughput two-hybrid screen;
YOR097C is not an essential gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014740.1"
/db_xref="GeneID:854264"
/db_xref="SGD:S000005623"
/translation="MDLKRDWLRWKITIGSGPGSIVLDFPSFLVGCVFTTMMGPILQK
LIGKLLVGLITVCKFLVIIGSIVFVIGVASKKYTYDDFKVSIKRSGEPGESHDMRTEP
KRTAKTATVPMEKDEGVGSYNYFEIPITKETSTIPYINCDGTSSLRKPPNGPSSVGLS
NSNRYENFINMARHK"
gene complement(<507948..>511178)
/gene="NUP1"
/locus_tag="YOR098C"
/db_xref="GeneID:854265"
mRNA complement(<507948..>511178)
/gene="NUP1"
/locus_tag="YOR098C"
/product="FG-nucleoporin NUP1"
/transcript_id="NM_001183517.1"
/db_xref="GeneID:854265"
CDS complement(507948..511178)
/gene="NUP1"
/locus_tag="YOR098C"
/experiment="EXISTENCE:direct assay:GO:0005643 nuclear
pore [PMID:2190694|PMID:11046143|PMID:10684247]"
/experiment="EXISTENCE:direct assay:GO:0044613 nuclear
pore central transport channel [PMID:18046406]"
/experiment="EXISTENCE:direct assay:GO:0044615 nuclear
pore nuclear basket [PMID:10684247]"
/experiment="EXISTENCE:genetic interaction:GO:0006607
NLS-bearing protein import into nucleus [PMID:15039779]"
/experiment="EXISTENCE:mutant phenotype:GO:0000055
ribosomal large subunit export from nucleus
[PMID:11071906]"
/experiment="EXISTENCE:mutant phenotype:GO:0000972
transcription-dependent tethering of RNA polymerase II
gene DNA at nuclear periphery
[PMID:20098417|PMID:16760982]"
/experiment="EXISTENCE:mutant phenotype:GO:0006606 protein
import into nucleus [PMID:7929578]"
/experiment="EXISTENCE:mutant phenotype:GO:0006607
NLS-bearing protein import into nucleus
[PMID:16118201|PMID:11046143]"
/experiment="EXISTENCE:mutant phenotype:GO:0016973
poly(A)+ mRNA export from nucleus
[PMID:12411502|PMID:16118201|PMID:7929578]"
/experiment="EXISTENCE:mutant phenotype:GO:0017056
structural constituent of nuclear pore [PMID:17418788]"
/experiment="EXISTENCE:physical interaction:GO:0017056
structural constituent of nuclear pore
[PMID:12917401|PMID:17418788|PMID:10525531]"
/note="FG-nucleoporin component of central core of the
nuclear pore complex; contributes directly to
nucleocytoplasmic transport and maintenance of thenuclear
pore complex (NPC) permeability barrier; possible
karyopherin release factor that accelerates release of
karyopherin-cargo complexes after transport across NPC;
both NUP1 and NUP60 are homologous to human NUP153"
/codon_start=1
/product="FG-nucleoporin NUP1"
/protein_id="NP_014741.1"
/db_xref="GeneID:854265"
/db_xref="SGD:S000005624"
/translation="MSSNTSSVMSSPRVEKRSFSSTLKSFFTNPNKKRPSSKKVFSSN
LSYANHLEESDVEDTLHVNKRKRVSGTSQHSDSLTQNNNNAPIIIYGTENTERPPLLP
ILPIQRLRLLREKQRVRNMRELGLIQSTEFPSITSSVILGSQSKSDEGGSYLCTSSTP
SPIKNGSCTRQLAGKSGEDTNVGLPILKSLKNRSNRKRFHSQSKGTVWSANFEYDLSE
YDAIQKKDNKDKEGNAGGDQKTSENRNNIKSSISNGNLATGPNLTSEIEDLRADINSN
RLSNPQKNLLLKGPASTVAKTAPIQESFVPNSERSGTPTLKKNIEPKKDKESIVLPTV
GFDFIKDNETPSKKTSPKATSSAGAVFKSSVEMGKTDKSTKTAEAPTLSFNFSQKANK
TKAVDNTVPSTTLFNFGGKSDTVTSASQPFKFGKTSEKSENHTESDAPPKSTAPIFSF
GKQEENGDEGDDENEPKRKRRLPVSEDTNTKPLFDFGKTGDQKETKKGESEKDASGKP
SFVFGASDKQAEGTPLFTFGKKADVTSNIDSSAQFTFGKAATAKETHTKPSETPATIV
KKPTFTFGQSTSENKISEGSAKPTFSFSKSEEERKSSPISNEAAKPSFSFPGKPVDVQ
APTDDKTLKPTFSFTEPAQKDSSVVSEPKKPSFTFASSKTSQPKPLFSFGKSDAAKEP
PGSNTSFSFTKPPANETDKRPTPPSFTFGGSTTNNTTTTSTKPSFSFGAPESMKSTAS
TAAANTEKLSNGFSFTKFNHNKEKSNSPTSFFDGSASSTPIPVLGKPTDATGNTTSKS
AFSFGTANTNGTNASANSTSFSFNAPATGNGTTTTSNTSGTNIAGTFNVGKPDQSIAS
GNTNGAGSAFGFSSSGTAATGAASNQSSFNFGNNGAGGLNPFTSATSSTNANAGLFNK
PPSTNAQNVNVPSAFNFTGNNSTPGGGSVFNMNGNTNANTVFAGSNNQPHQSQTPSFN
TNSSFTPSTVPNINFSGLNGGITNTATNALRPSDIFGANAASGSNSNVTNPSSIFGGA
GGVPTTSFGQPQSAPNQMGMGTNNGMSMGGGVMANRKIARMRHSKR"
gene <511825..>513006
/gene="KTR1"
/locus_tag="YOR099W"
/db_xref="GeneID:854266"
mRNA <511825..>513006
/gene="KTR1"
/locus_tag="YOR099W"
/product="alpha-1,2-mannosyltransferase KTR1"
/transcript_id="NM_001183518.1"
/db_xref="GeneID:854266"
CDS 511825..513006
/gene="KTR1"
/locus_tag="YOR099W"
/experiment="EXISTENCE:direct assay:GO:0000026
alpha-1,2-mannosyltransferase activity [PMID:9020857]"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:22842922|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:8631921]"
/experiment="EXISTENCE:genetic interaction:GO:0006487
protein N-linked glycosylation [PMID:9182588]"
/experiment="EXISTENCE:genetic interaction:GO:0006493
protein O-linked glycosylation [PMID:9182588]"
/experiment="EXISTENCE:mutant phenotype:GO:0000026
alpha-1,2-mannosyltransferase activity [PMID:9182588]"
/note="Alpha-1,2-mannosyltransferase; involved in O- and
N-linked protein glycosylation; type II membrane protein;
member of the KRE2/MNT1 mannosyltransferase family;
relocalizes from vacuole to cytoplasm upon DNA replication
stress"
/codon_start=1
/product="alpha-1,2-mannosyltransferase KTR1"
/protein_id="NP_014742.1"
/db_xref="GeneID:854266"
/db_xref="SGD:S000005625"
/translation="MAKIMIPASKQPVYKKLGLLLVAVFTVYVFFHGAQYARGSAPSP
KYSTVLSSGSGYKYSKVELPKYTGPREKATFVTLVRNRDLYSLAESIKSVEDRFNSKF
NYDWVFLNDEEFTDEFKNVTSALVSGTTKYGVIPKEHWSFPEWIDEEKAAQVRKEMGE
KRIIYGDSISYRHMCRFESGFFYRHPLMDDYDWYWRVEPDIKLHCDIDYDVFKFMKDN
KKKYAFAISIKEYEATIPTLWETTRKFMEAHPELIHENNMLDFVSDDQGLSYNLCHFW
SNFEIAALDLWRSPAYSAYFDYLDREGGFFYERWGDAPVHSIGAALFLDRSEIHHFGD
IGYYHVPFHSCPIDTSIRLANKCDCDPSKDFTWHSYSCTTKFYNINKLPKPAGWQNHI
G"
gene complement(<513295..>514278)
/gene="CRC1"
/locus_tag="YOR100C"
/db_xref="GeneID:854267"
mRNA complement(<513295..>514278)
/gene="CRC1"
/locus_tag="YOR100C"
/product="carnitine:acyl carnitine antiporter"
/transcript_id="NM_001183519.1"
/db_xref="GeneID:854267"
CDS complement(513295..514278)
/gene="CRC1"
/locus_tag="YOR100C"
/experiment="EXISTENCE:direct assay:GO:0005476
carnitine:O-acyl-L-carnitine antiporter activity
[PMID:10545096]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:11914276|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:10545096]"
/experiment="EXISTENCE:direct assay:GO:0006631 fatty acid
metabolic process [PMID:10545096]"
/experiment="EXISTENCE:direct assay:GO:1902603 carnitine
transmembrane transport [PMID:10545096]"
/note="Mitochondrial inner membrane carnitine transporter;
required for carnitine-dependent transport of acetyl-CoA
from peroxisomes to mitochondria during fatty acid
beta-oxidation; human homolog SLC25A20 complements yeast
null mutant"
/codon_start=1
/product="carnitine:acyl carnitine antiporter"
/protein_id="NP_014743.1"
/db_xref="GeneID:854267"
/db_xref="SGD:S000005626"
/translation="MSSDTSLSESSLLKEESGSLTKSRPPIKSNPVRENIKSFVAGGV
GGVCAVFTGHPFDLIKVRCQNGQANSTVHAITNIIKEAKTQVKGTLFTNSVKGFYKGV
IPPLLGVTPIFAVSFWGYDVGKKLVTFNNKQGGSNELTMGQMAAAGFISAIPTTLVTA
PTERVKVVLQTSSKGSFIQAAKTIVKEGGIASLFKGSLATLARDGPGSALYFASYEIS
KNYLNSRQPRQDAGKDEPVNILNVCLAGGIAGMSMWLAVFPIDTIKTKLQASSTRQNM
LSATKEIYLQRGGIKGFFPGLGPALLRSFPANAATFLGVEMTHSLFKKYGI"
gene <515244..>516173
/gene="RAS1"
/locus_tag="YOR101W"
/db_xref="GeneID:854268"
mRNA <515244..>516173
/gene="RAS1"
/locus_tag="YOR101W"
/product="Ras family GTPase RAS1"
/transcript_id="NM_001183520.1"
/db_xref="GeneID:854268"
CDS 515244..516173
/gene="RAS1"
/locus_tag="YOR101W"
/EC_number="3.6.5.2"
/experiment="EXISTENCE:direct assay:GO:0003924 GTPase
activity [PMID:14674766]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:20162532|PMID:3513173]"
/experiment="EXISTENCE:direct assay:GO:0007189 adenylate
cyclase-activating G protein-coupled receptor signaling
pathway [PMID:3891097]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0045762
positive regulation of adenylate cyclase activity
[PMID:3891097]"
/experiment="EXISTENCE:genetic interaction:GO:0097271
protein localization to bud neck [PMID:12782684]"
/experiment="EXISTENCE:mutant phenotype:GO:0003924 GTPase
activity [PMID:6327067]"
/note="GTPase involved in G-protein signaling in adenylate
cyclase activation; plays a role in regulation of cell
proliferation; localized to the plasma membrane; activated
by increased levels of glycolysis intermediate
fructose-1,6-bisphosphate; homolog of mammalian RAS
proto-oncogenes; relative distribution to the nucleus
increases upon DNA replication stress; RAS1 has a paralog,
RAS2, that arose from the whole genome duplication"
/codon_start=1
/product="Ras family GTPase RAS1"
/protein_id="NP_014744.1"
/db_xref="GeneID:854268"
/db_xref="SGD:S000005627"
/translation="MQGNKSTIREYKIVVVGGGGVGKSALTIQFIQSYFVDEYDPTIE
DSYRKQVVIDDKVSILDILDTAGQEEYSAMREQYMRTGEGFLLVYSVTSRNSFDELLS
YYQQIQRVKDSDYIPVVVVGNKLDLENERQVSYEDGLRLAKQLNAPFLETSAKQAINV
DEAFYSLIRLVRDDGGKYNSMNRQLDNTNEIRDSELTSSATADREKKNNGSYVLDNSL
TNAGTGSSSKSAVNHNGETTKRTDEKNYVNQNNNNEGNTKYSSNGNGNRSDISRGNQN
NALNSRSKQSAEPQKNSSANARKESSGGCCIIC"
gene complement(<516449..>516841)
/gene="OST2"
/locus_tag="YOR103C"
/db_xref="GeneID:854270"
mRNA complement(<516449..>516841)
/gene="OST2"
/locus_tag="YOR103C"
/product="dolichyl-diphosphooligosaccharide-protein
glycotransferase"
/transcript_id="NM_001183522.1"
/db_xref="GeneID:854270"
CDS complement(516449..516841)
/gene="OST2"
/locus_tag="YOR103C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0008250
oligosaccharyltransferase complex [PMID:10358084]"
/experiment="EXISTENCE:mutant phenotype:GO:0006487 protein
N-linked glycosylation [PMID:7593165]"
/experiment="EXISTENCE:physical interaction:GO:0006487
protein N-linked glycosylation [PMID:9405463]"
/experiment="EXISTENCE:physical interaction:GO:0008250
oligosaccharyltransferase complex [PMID:9405463]"
/note="Non-catalytic epsilon subunit of the
oligosaccharyltransferase (OST) complex; the OST complex
catalyzes asparagine-linked glycosylation of newly
synthesized proteins in the ER; forms a pocket with Wbp1p
involved in binding terminal glucose units of the donor
glycan; located in the ER lumen"
/codon_start=1
/product="dolichyl-diphosphooligosaccharide-protein
glycotransferase"
/protein_id="NP_014746.2"
/db_xref="GeneID:854270"
/db_xref="SGD:S000005629"
/translation="MAKAPKANTPKVTSTSSAVLTDFQETFKTSKRAYFAQIEKYPKL
KLIDTFCFFLVLLGVIQCTFIILIRDNFPFNAFLAGFIICVGQFVLLMSLRLQLCNSF
PGISKNRAFAEFIVASLILHFVCLHFIN"
gene <517642..>518490
/gene="PIN2"
/locus_tag="YOR104W"
/db_xref="GeneID:854271"
mRNA <517642..>518490
/gene="PIN2"
/locus_tag="YOR104W"
/product="Pin2p"
/transcript_id="NM_001183523.1"
/db_xref="GeneID:854271"
CDS 517642..518490
/gene="PIN2"
/locus_tag="YOR104W"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:24656818]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:24656818]"
/note="Exomer-dependent cargo protein; induces appearance
of [PIN+] prion when overproduced; prion-like domain
serves as a retention signal in the trans-Golgi network;
predicted to be palmitoylated"
/codon_start=1
/product="Pin2p"
/protein_id="NP_014747.1"
/db_xref="GeneID:854271"
/db_xref="SGD:S000005630"
/translation="MNVCKLKEIVPLFPRSSFTDGVVSTGKSFRSWDTCMDNKACKII
AIVGIVLACILVIWLIGGLLTCFRQGVTGIGQFICWCCRCSNDRNGNNTMPVNEGFSR
VNMGVAPPSTVIYQPIQQPESAYYRNDAKNDTFYDEVKTPSNEVYELEEDFDLEKQKE
KTRKKQQKERNKEGRSPSRVAPLVYEEENFEGSSPQPQYDARNSFIQNAANTGSNNAH
VASQSPIFDISDYGENYYYDNNNINNNLQGNSYNTPSSNHRSPYPTENYQSYQGYKPN
QSDRYY"
gene <518195..>518521
/locus_tag="YOR105W"
/db_xref="GeneID:854272"
mRNA <518195..>518521
/locus_tag="YOR105W"
/product="uncharacterized protein"
/transcript_id="NM_001270761.1"
/db_xref="GeneID:854272"
CDS 518195..518521
/locus_tag="YOR105W"
/note="hypothetical protein; expressed at both mRNA and
protein levels"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001257690.1"
/db_xref="GeneID:854272"
/db_xref="SGD:S000005631"
/translation="MKKKISKVAALSRNMMLEIRLSKMRPILVATTLMLLHNLQFLTS
AIMARTIITIIITSIITSRETVTILPPLIIEVHIQPKIINHIRDINQTKAIDITNEVN
IYSPFF"
gene <519121..>519972
/gene="VAM3"
/locus_tag="YOR106W"
/gene_synonym="PTH1"
/db_xref="GeneID:854273"
mRNA <519121..>519972
/gene="VAM3"
/locus_tag="YOR106W"
/gene_synonym="PTH1"
/product="SNAP receptor VAM3"
/transcript_id="NM_001183525.1"
/db_xref="GeneID:854273"
CDS 519121..519972
/gene="VAM3"
/locus_tag="YOR106W"
/gene_synonym="PTH1"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:9202390|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005484 SNAP
receptor activity [PMID:17054985]"
/experiment="EXISTENCE:direct assay:GO:0006906 vesicle
fusion [PMID:11001046]"
/experiment="EXISTENCE:direct assay:GO:0031201 SNARE
complex [PMID:9425154]"
/experiment="EXISTENCE:direct assay:GO:0042144 vacuole
fusion, non-autophagic [PMID:9685264]"
/experiment="EXISTENCE:genetic interaction:GO:0048278
vesicle docking [PMID:9245783]"
/experiment="EXISTENCE:genetic interaction:GO:0051469
vesicle fusion with vacuole [PMID:9245783]"
/experiment="EXISTENCE:mutant phenotype:GO:0034727
piecemeal microautophagy of the nucleus [PMID:18701704]"
/experiment="EXISTENCE:mutant phenotype:GO:0097352
autophagosome maturation [PMID:9202390]"
/note="Vacuolar membrane protein and Qa class t-SNARE;
syntaxin-like vacuolar t-SNARE that functions with Vam7p
in vacuolar protein trafficking; mediates docking/fusion
of late transport intermediates with the vacuole; has an
acidic di-leucine sorting signal and C-terminal
transmembrane region"
/codon_start=1
/product="SNAP receptor VAM3"
/protein_id="NP_014749.1"
/db_xref="GeneID:854273"
/db_xref="SGD:S000005632"
/translation="MSFFDIEAQSSKGNSQQEPQFSTNQKTKELSNLIETFAEQSRVL
EKECTKIGSKRDSKELRYKIETELIPNCTSVRDKIESNILIHQNGKLSADFKNLKTKY
QSLQQSYNQRKSLFPLKTPISPGTSKERKDIHPRTEAVRQDPESSYISIKVNEQSPLL
HNEGQHQLQLQEEQEQQQQGLSQEELDFQTIIHQERSQQIGRIHTAVQEVNAIFHQLG
SLVKEQGEQVTTIDENISHLHDNMQNANKQLTRADQHQRDRNKCGKVTLIIIIVVCMV
VLLAVLS"
gene <521353..>522282
/gene="RGS2"
/locus_tag="YOR107W"
/db_xref="GeneID:854274"
mRNA <521353..>522282
/gene="RGS2"
/locus_tag="YOR107W"
/product="GTPase-activating protein RGS2"
/transcript_id="NM_001183526.3"
/db_xref="GeneID:854274"
CDS 521353..522282
/gene="RGS2"
/locus_tag="YOR107W"
/experiment="EXISTENCE:direct assay:GO:0005096 GTPase
activator activity [PMID:10523302]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0007188
adenylate cyclase-modulating G protein-coupled receptor
signaling pathway [PMID:10523302]"
/note="Negative regulator of glucose-induced cAMP
signaling; directly activates the GTPase activity of the
heterotrimeric G protein alpha subunit Gpa2p"
/codon_start=1
/product="GTPase-activating protein RGS2"
/protein_id="NP_014750.3"
/db_xref="GeneID:854274"
/db_xref="SGD:S000005633"
/translation="MASVPSLCDILIPLEKNSRSDGDAESSNTVLIQLRKGHHERMRS
PYTIQKFYKFLKRAHCEENLEFFEKAHQFLQLKQNRSISEEKLLEVWNKSLYIKYIAV
DSPKECNFSQDTREIFEKCFANNEVPADVDVLCAISHVMGLLMDGYHRFVSSVNEKKY
SATYAHNDSATEQDLKNESTTSFSSLGVEDISEDRNSYLKKPDINGLSTIIQETSANT
TNESQCSDRTSRPSESSSSLNTTSSTYKNTSTRNLQKPQNTGILNSGKGLLQKLNFVK
KRKSFKQPSGVICSHYNSNVQNRLKGQNSHQQR"
gene <523027..>524841
/gene="LEU9"
/locus_tag="YOR108W"
/db_xref="GeneID:854275"
mRNA <523027..>524841
/gene="LEU9"
/locus_tag="YOR108W"
/product="2-isopropylmalate synthase LEU9"
/transcript_id="NM_001183527.1"
/db_xref="GeneID:854275"
CDS 523027..524841
/gene="LEU9"
/locus_tag="YOR108W"
/EC_number="2.3.3.13"
/experiment="EXISTENCE:direct assay:GO:0003852
2-isopropylmalate synthase activity
[PMID:11158296|PMID:25841022]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:11158296|PMID:16823961]"
/experiment="EXISTENCE:genetic interaction:GO:0003852
2-isopropylmalate synthase activity [PMID:10790691]"
/experiment="EXISTENCE:genetic interaction:GO:0009098
L-leucine biosynthetic process [PMID:10790691]"
/experiment="EXISTENCE:mutant phenotype:GO:0009098
L-leucine biosynthetic process [PMID:25841022]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:25841022]"
/note="Alpha-isopropylmalate synthase II
(2-isopropylmalate synthase); catalyzes the first step in
the leucine biosynthesis pathway; the minor isozyme,
responsible for the residual alpha-IPMS activity detected
in a leu4 null mutant; LEU9 has a paralog, LEU4, that
arose from the whole genome duplication"
/codon_start=1
/product="2-isopropylmalate synthase LEU9"
/protein_id="NP_014751.1"
/db_xref="GeneID:854275"
/db_xref="SGD:S000005634"
/translation="MVKHSFIALAEHASKLRRSIPPVKLTYKNMLRDPSVKYRAFAPP
KMVKRIWPDKTIQKAPRWLSTDLRDGNQSLPDPMSVAQKKEYFHKLINIGFKEIEVSF
PSASQTDFDFTRYAVENAPDDVGIQCLVQSREHLIKRTVEALTGAKRATIHTYLATSD
MFREIVFNMSREEAISKAVEATKLVRKLTKDDPSQQATRWSYEFSPECFSDTPGEFAV
EICEAVKKAWEPTEENPIIFNLPATVEVASPNVYADQIEYFSTHITEREKVCISTHCH
NDRGCGVAATELGMLAGADRVEGCLFGNGERTGNVDLVTVAMNMYTQGVSPNLDFSDL
TSISEIVHRCNKIPIPPRAPYGGELVVSAFSGSHQDAIKKGFAIQNKKQAQGETRWRI
PYLPLDPKDIGRDYEAVIRVNSQSGKGGAAWVIMRSLGLDVPRPMQVDFSNTLQKNAD
ALGRELKSEEITKLFKETYNYNNNEHIYVTLLNYEVKKLNPERRALVGQVEINDKVVN
IEGYGNGPISSLVDALSNLLNVKLSVQNYSEHSLGSGSATQAASFINLSYIKDINNHA
TSNMWGVGVSEDTGDASIKAVFATVNNIIHSGDVLLAE"
gene <525278..>528601
/gene="INP53"
/locus_tag="YOR109W"
/gene_synonym="SJL3; SOP2"
/db_xref="GeneID:854276"
mRNA <525278..>528601
/gene="INP53"
/locus_tag="YOR109W"
/gene_synonym="SJL3; SOP2"
/product="phosphatidylinositol-3-/phosphoinositide
5-phosphatase INP53"
/transcript_id="NM_001183528.3"
/db_xref="GeneID:854276"
CDS 525278..528601
/gene="INP53"
/locus_tag="YOR109W"
/gene_synonym="SJL3; SOP2"
/EC_number="3.1.3.36"
/experiment="EXISTENCE:direct assay:GO:0004438
phosphatidylinositol-3-phosphate phosphatase activity
[PMID:10224048]"
/experiment="EXISTENCE:direct assay:GO:0004439
phosphatidylinositol-4,5-bisphosphate 5-phosphatase
activity [PMID:10224048]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11094088]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:9560389]"
/experiment="EXISTENCE:direct assay:GO:0030479 actin
cortical patch [PMID:11094088]"
/experiment="EXISTENCE:direct assay:GO:0043812
phosphatidylinositol-4-phosphate phosphatase activity
[PMID:10224048]"
/experiment="EXISTENCE:direct assay:GO:0043813
phosphatidylinositol-3,5-bisphosphate 5-phosphatase
activity [PMID:10224048]"
/experiment="EXISTENCE:direct assay:GO:0046856
phosphatidylinositol dephosphorylation [PMID:10224048]"
/experiment="EXISTENCE:mutant phenotype:GO:0004439
phosphatidylinositol-4,5-bisphosphate 5-phosphatase
activity [PMID:9438131]"
/experiment="EXISTENCE:mutant phenotype:GO:0046856
phosphatidylinositol dephosphorylation [PMID:9438131]"
/note="Polyphosphatidylinositol phosphatase;
dephosphorylates multiple phosphatidylinositol phosphates;
involved in trans Golgi network-to-early endosome pathway;
hyperosmotic stress causes translocation to actin patches;
contains Sac1 and 5-ptase domains; INP53 has a paralog,
INP52, that arose from the whole genome duplication"
/codon_start=1
/product="phosphatidylinositol-3-/phosphoinositide
5-phosphatase INP53"
/protein_id="NP_014752.3"
/db_xref="GeneID:854276"
/db_xref="SGD:S000005635"
/translation="MIIFVSEEPERRLAIVSNLYALVLKPVGKKPSDKPLCAIELLQK
NDLKKYGFKRLTSHEIFGVIGLIEVNGLLFVGAITGKSKVAQPCPGETVNKIFAVDFF
CLNDNSWDFIEIDSSGYPVLPETASTEYQDALPKHPCYELKKLLSNGSFYYSSDFDLT
STLQHRGYGQHSLSTDTYEEEYMWNSFLMQEMITYRDHLDTNLKQILDDEGFLTTVIR
GFAETFVSYVKKLKVALTIISKQSWKRAGTRFNARGVDDEANVANFVETEFIMYSSQY
CYAFTQIRGSIPVFWEQGTSLINPRVQITRSFEATQPVFDKHIMKSVEKYGPVHVVNL
LSTKSSEIELSKRYKEHLTHSKKLNFNKDIFLTEFDFHKETSQEGFSGVRKLIPLILD
SLLSSGYYSYDVREKKNISEQHGIFRTNCLDCLDRTNLAQQIISLAAFRTFLEDFRLI
SSNSFIDDDDFVSKHNTLWADHGDQISQIYTGTNALKSSFSRKGKMSLAGALSDATKS
VSRIYINNFMDKEKQQNIDTLLGRLPYQKAVQLYDPVNEYVSTKLQSMSDKFTSTSNI
NLLIGSFNVNGATKKVDLSKWLFPIGEKFKPDIVVLGLQEVIELSAGSILNADYSKSS
FWENLVGDCLNQYDDKYLLLRVEQMTSLLILFFVKADKAKYVKQVEGATKKTGFRGMA
GNKGAVSIRFEYGATSFCFVNSHLAAGATNVEERRSDYESIVRGITFTRTKMIPHHDS
IFWLGDMNYRINLPNEDVRRELLNQEEGYIDKLLHFDQLTLGINSGSVFEGFKEPTLK
FRPTYKYDPGTGTYDSSEKERTPSWTDRIIYKGENLLPLSYSDAPIMISDHRPVYAAY
RAKITFVDDKERLSLKKRLFTEYKQEHPEEPGSLISDLLSLDLDNKSTDGFKSSSESS
LLDIDPIMAQPTASSVASSSPVSSASASLQPVRTQNSSQSRTPIKKPVLRPPPPPAHK
SVSAPAPSTSKEKSPTPQTSTASLSSVTKNIQENKPLAQNRRIPPPGFSQNILTPKST
SNLASPMSSKVDLYNSASESTRSAQDARQQTPTAFAASRDVNGQPEALLGDENPIEPE
EKAKLNHMTLDSWQPLTPK"
gene <528941..>530248
/gene="TFC7"
/locus_tag="YOR110W"
/db_xref="GeneID:854277"
mRNA <528941..>530248
/gene="TFC7"
/locus_tag="YOR110W"
/product="transcription factor TFIIIC subunit TFC7"
/transcript_id="NM_001183529.1"
/db_xref="GeneID:854277"
CDS 528941..530248
/gene="TFC7"
/locus_tag="YOR110W"
/experiment="EXISTENCE:direct assay:GO:0000127
transcription factor TFIIIC complex
[PMID:9584160|PMID:1474578|PMID:2002052]"
/experiment="EXISTENCE:direct assay:GO:0000995 RNA
polymerase III general transcription initiation factor
activity [PMID:9584160]"
/experiment="EXISTENCE:direct assay:GO:0001002 RNA
polymerase III type 1 promoter sequence-specific DNA
binding [PMID:2649882|PMID:2404611]"
/experiment="EXISTENCE:direct assay:GO:0001003 RNA
polymerase III type 2 promoter sequence-specific DNA
binding [PMID:2404611]"
/experiment="EXISTENCE:direct assay:GO:0006383
transcription by RNA polymerase III
[PMID:2183033|PMID:8464480|PMID:9584160|PMID:3633923]"
/experiment="EXISTENCE:direct assay:GO:0008301 DNA
binding, bending [PMID:2002052]"
/experiment="EXISTENCE:direct assay:GO:0016791 phosphatase
activity [PMID:23569204]"
/experiment="EXISTENCE:direct assay:GO:0042791 5S class
rRNA transcription by RNA polymerase III [PMID:2183033]"
/experiment="EXISTENCE:mutant phenotype:GO:0000995 RNA
polymerase III general transcription initiation factor
activity [PMID:9584160]"
/experiment="EXISTENCE:mutant phenotype:GO:0006383
transcription by RNA polymerase III [PMID:9584160]"
/note="RNA pol III transcription initiation factor complex
(TFIIIC) subunit; part of the TauA globular domain of
TFIIIC that binds DNA at the BoxA promoter sites of tRNA
and similar genes; TFC7 has a paralog, YNL108C, that arose
from the whole genome duplication"
/codon_start=1
/product="transcription factor TFIIIC subunit TFC7"
/protein_id="NP_014753.1"
/db_xref="GeneID:854277"
/db_xref="SGD:S000005636"
/translation="MVVNTIYIARHGYRSNWLPEGPYPDPLTGIDSDVPLAEHGVQQA
KELAHYLLSLDNQPEAAFASPFYRCLETVQPIAKLLEIPVYLERGIGEWYRPDRKPVI
PVPAGYEILSKFFPGVISQEWDSTLTPNEKGETEQEMYMRFKKFWPLFIERVEKEYPN
VECILLVTHAASKIALGMSLLGYDNPRMSLNENGDKIRSGSCSLDKYEILKKSYDTID
ETDDQTSFTYIPFSDRKWVLTMNGNTEFLSSGEEMNWNFDCVAEAGSDADIKKRQMTK
KTSSPIPEADDQTEVETVYISVDIPSGNYKERTEIAKSAILQYSGLETDAPLFRIGNR
LYEGSWERLVGTELAFPNAAHVHKKTAGLLSPTEENETTNAGQSKGSSTANDPNIQIQ
EEDVGLPDSTNTSRDHTGDKEEVQSEKIYRIKERIVLSNVRPM"
gene <530429..>531127
/locus_tag="YOR111W"
/db_xref="GeneID:854278"
mRNA <530429..>531127
/locus_tag="YOR111W"
/product="nucleotide diphosphatase"
/transcript_id="NM_001183530.3"
/db_xref="GeneID:854278"
CDS 530429..531127
/locus_tag="YOR111W"
/EC_number="3.6.1.9"
/note="hypothetical protein"
/codon_start=1
/product="nucleotide diphosphatase"
/protein_id="NP_014754.3"
/db_xref="GeneID:854278"
/db_xref="SGD:S000005637"
/translation="MSGNSQLPPDVIGFICSKYDIILASTSPRRYEILHDIMGITDLK
TMVSTFEENLDKMNYSTDPIGYVCDTSWHKAQNIIEILTDYEDENPNEIDKPKLIICA
DTIIIDKSGRIYEKPKTKEVQKKFLMKFCYEDDEPVNVVTAVTLIKWYGRENFELVPF
RDETKVYFDNKIPLRILEEYVESGDGLEVGGGFKIQGQGAILIEKIEGDYYNVVGLPL
NKTFKGLYAEANSI"
gene <531508..>533793
/gene="CEX1"
/locus_tag="YOR112W"
/db_xref="GeneID:854279"
mRNA <531508..>533793
/gene="CEX1"
/locus_tag="YOR112W"
/product="COPI-interacting protein CEX1"
/transcript_id="NM_001183531.1"
/db_xref="GeneID:854279"
CDS 531508..533793
/gene="CEX1"
/locus_tag="YOR112W"
/experiment="EXISTENCE:direct assay:GO:0000049 tRNA
binding [PMID:17203074]"
/experiment="EXISTENCE:direct assay:GO:0005643 nuclear
pore [PMID:17203074]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:17203074]"
/experiment="EXISTENCE:genetic interaction:GO:0006409 tRNA
export from nucleus [PMID:22008473|PMID:17203074]"
/experiment="EXISTENCE:mutant phenotype:GO:0006409 tRNA
export from nucleus [PMID:17203074]"
/experiment="EXISTENCE:mutant phenotype:GO:0006890
retrograde vesicle-mediated transport, Golgi to
endoplasmic reticulum [PMID:33753324]"
/experiment="EXISTENCE:physical interaction:GO:0005643
nuclear pore [PMID:17203074]"
/experiment="EXISTENCE:physical interaction:GO:0006409
tRNA export from nucleus [PMID:17203074]"
/note="Component of COPI-mediated trafficking and tRNA
nuclear export; enables Rna1p to access and activate
Gsp1p-GTP bound to the export receptor tRNA complex during
aminoacylation-dependent tRNA export; copurifies with tRNA
export receptors; interacts with COPI coat proteins and
regulates Golgi-to-ER trafficking; membrane fraction
associated; mutations in members of the homologous human
SCY1-like (SCYL) family of pseudokinases are linked to
peripheral neuropathy, cerebellar atrophy, ataxia and ALS"
/codon_start=1
/product="COPI-interacting protein CEX1"
/protein_id="NP_014755.1"
/db_xref="GeneID:854279"
/db_xref="SGD:S000005638"
/translation="MNFSSIFKSISNFQFPYTIEETAITETALWQCFDGTRKADSLPV
TVFKAKRSPENESLILNAVHKSKILKIPGLCTVLETFDSDPQSTFIVTERVVPFPWDN
LGSLSQNKFGVELGISQLLATLGFLKNFVLGTLSKDSVFINIKGEWVLFGLELCSSKE
GLSAFEFASRARSYYNIIGSQLPCEDPNTIDSMGLGLLIKSLMAPSCLPKDWIVNVNM
ISDGKITIENFRKRLENTETWRSNPLINFYQELRELHIKDPQGKLVVMSNLENLYLES
REIFRNLTPGMIENFIIPELCEIIKLLMTQSISSAASPIGMNFNASHKLVPFLAIVLD
LTSETNTFPVGFNDLITQSFKLPDRQVRFLLLIYLPKLIGPLSKSEISSRIYPHFIQG
LTDSDATLRLQTLKTIPCIVSCLTERQLNNELLRFLAKTQVDSDVEIRTWTVIIISKI
STILSTSVGNRSNILATAFTKSLKDPQVKPRLAALYGLEKSIELFDVNTIANKILTVI
APGLLDKSPIVRGRAKILFEEYLEKLEKEAQLIQTNDSTADSEDVKDIDFENYGCDEE
DMNKEDNLLAAQFLNNLRLNSPSATTPSNITESEIDSAQDGSGWDDLSDTDGFITNGT
TESFDETTNPVTTASTPKLFGKPIKINKSWNDELNDDGWIQDESGPSKVPQKHTRPQN
STLAKSIAPSSRLSIKKKKTTILAPRNIASNSTVTTKSSLSNKTARSKPISSIRGSVT
KKGNVDGWDDDGDSDSWDTNW"
gene <534075..>536819
/gene="AZF1"
/locus_tag="YOR113W"
/db_xref="GeneID:854280"
mRNA <534075..>536819
/gene="AZF1"
/locus_tag="YOR113W"
/product="Azf1p"
/transcript_id="NM_001183532.3"
/db_xref="GeneID:854280"
CDS 534075..536819
/gene="AZF1"
/locus_tag="YOR113W"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:16467472|PMID:11839825]"
/experiment="EXISTENCE:direct assay:GO:0001228 DNA-binding
transcription activator activity, RNA polymerase
II-specific [PMID:11839825]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9799362|PMID:16467472|PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0006357 regulation
of transcription by RNA polymerase II [PMID:11839825]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:19111667]"
/experiment="EXISTENCE:mutant phenotype:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:16467472]"
/experiment="EXISTENCE:mutant phenotype:GO:0001228
DNA-binding transcription activator activity, RNA
polymerase II-specific [PMID:11839825]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:11839825]"
/experiment="EXISTENCE:mutant phenotype:GO:0060237
regulation of fungal-type cell wall organization
[PMID:16467472]"
/experiment="EXISTENCE:mutant phenotype:GO:0071322
cellular response to carbohydrate stimulus
[PMID:16467472|PMID:11839825]"
/note="Zinc-finger transcription factor; involved in the
diauxic shift; activates transcription of genes involved
in carbon metabolism and energy production on glucose,
while it activates transcription of genes involved in cell
wall organization and biogenesis on glycerol-lactate; may
also function as a co-repressor; capable of forming the
prion [AZF1+]; relocalizes to the cytosol in response to
hypoxia; contains polyglutamine and polyasparagine
domains"
/codon_start=1
/product="Azf1p"
/protein_id="NP_014756.3"
/db_xref="GeneID:854280"
/db_xref="SGD:S000005639"
/translation="MPPPTAQFMGPTQAGQNESQNQSSGEAGEQNQEHGQGPTPILNQ
SQPASSQPQHQQQRNESISYYTNFNQPRYSTDASINSFLNISDNVPVTSTGGPSSGGA
YSNLPRLSTSSTHQPPDLSQIGRGFSIVNNLFPQQQQLQNQHRQQQQQQQQQSHQQPP
FKTPSFSTGLTGSSSQYQFLPRNDNTSQPPSKRNSVYLGPNDGPDFEFFSMQQSQQPQ
FQPSSRRESNSMRPPLLIPAATTKSQSNGTNNSGNMNTNADYESFFNTGTNNSNSNQN
PYFLSSRNNSLKFNPEDFDFQFKRRNSFVRGTLDHSSQNAFIPESRLNSLSVNNKANG
DPVADNVTNNMKGKSNEVDNDDGNDSSNNNNNNNNNNNNENNNDNNNDNNDNSINSAT
STNIPNQEDHSLASTDTTSNSRKDLKEIEQRLRKHLNDEDNYSSAISRPLDKNDVIEG
SEGLNKHIDESGMQPNIIKKRKKDDSTVYVKNEMPRTDPPMSKDNSTSAEGAAMANFS
GKEPPIPDISSVSDDATNLIGATKVDQLMLIIQARKKGFTEKVNTTQDGDLLFNQTMD
ILPPKSELVGGVEKPKGTQNTRAVKKHECPYCHRLFSQATHLEVHVRSHIGYKPFVCD
YCGKRFTQGGNLRTHERLHTGEKPYSCDICDKKFSRKGNLAAHLVTHQKLKPFVCKLE
NCNKTFTQLGNMKAHQNRFHKETLNALTAKLAEMNPSENIPLEERQLLEYFASIYKNS
NRGIKGRGKGVGTKKSTISSPENHPASTILNPNTNANNAIANDSENNGNPEGNIDSSS
NSNPGSHSMISPTQKDMGTLQSQFIQNNFNNSVNSSNPSNQPIINYNYTTLPHSRLGS
SSSSNTNNNNSNFSVGAAPGVLMAPTTNNDFSFNLDQSNDNERSQQEQVRFKNINYKS
"
gene <537570..>538454
/gene="CIM1"
/locus_tag="YOR114W"
/gene_synonym="DPI34"
/db_xref="GeneID:854281"
mRNA <537570..>538454
/gene="CIM1"
/locus_tag="YOR114W"
/gene_synonym="DPI34"
/product="mitochondrial HMG-box protein CIM1"
/transcript_id="NM_001183533.1"
/db_xref="GeneID:854281"
CDS 537570..538454
/gene="CIM1"
/locus_tag="YOR114W"
/gene_synonym="DPI34"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:37850632]"
/note="Mitochondrial HMG-box protein; limits the copy
number of mitochondrial DNA (mtDNA), antagonizing HMG-box
containing protein Abf2p, a mtDNA packaging factor;
localizes to the mitochondrial matrix; contains two
HMG-boxes similar to Abf2p; null mutant is viable"
/codon_start=1
/product="mitochondrial HMG-box protein CIM1"
/protein_id="NP_014757.1"
/db_xref="GeneID:854281"
/db_xref="SGD:S000005640"
/translation="MKATLLLKAQLSPVSYTTKKSFQRQLNRTPYTAFQYFFQLEVQK
LHNVSKYEDIINHVRGNSNFKRFARNEWDSMSLTKKRLYYASFCQSMDIDILNVSKIE
LAKRLEIPIPAMSEYLLFRNKFKVKFDSHCSSLERKDRKSVPRPSITRKVATTEICSK
SRSNTPVGKINPRKRLVALKRISRSENTAKNHSHEAQNYLYDYMKRFQQMCKECRYAW
NEEVDYDQKLEIRKKLQVWRAKFEEMMDNEIQILQKNMDIMSKFGLRSESYLTAANHD
TNTQPNNILPMTYLLKKK"
gene complement(<538659..>539465)
/gene="TRS33"
/locus_tag="YOR115C"
/db_xref="GeneID:854282"
mRNA complement(<538659..>539465)
/gene="TRS33"
/locus_tag="YOR115C"
/product="Trs33p"
/transcript_id="NM_001183534.3"
/db_xref="GeneID:854282"
CDS complement(538659..539465)
/gene="TRS33"
/locus_tag="YOR115C"
/experiment="EXISTENCE:direct assay:GO:0000407 phagophore
assembly site [PMID:27672095]"
/experiment="EXISTENCE:direct assay:GO:0005801 cis-Golgi
network [PMID:19843283]"
/experiment="EXISTENCE:direct assay:GO:0005802 trans-Golgi
network [PMID:19843283]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:1990070 TRAPPI
protein complex [PMID:11239471]"
/experiment="EXISTENCE:direct assay:GO:1990071 TRAPPII
protein complex [PMID:11239471]"
/experiment="EXISTENCE:direct assay:GO:1990072 TRAPPIII
protein complex [PMID:20375281]"
/experiment="EXISTENCE:genetic interaction:GO:0000407
phagophore assembly site [PMID:27672095]"
/experiment="EXISTENCE:genetic interaction:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:9564032]"
/experiment="EXISTENCE:genetic interaction:GO:0016239
positive regulation of macroautophagy [PMID:27672095]"
/experiment="EXISTENCE:genetic interaction:GO:0031503
protein-containing complex localization [PMID:27672095]"
/experiment="EXISTENCE:genetic interaction:GO:0034497
protein localization to phagophore assembly site
[PMID:27672095]"
/experiment="EXISTENCE:genetic interaction:GO:0065003
protein-containing complex assembly [PMID:19843283]"
/experiment="EXISTENCE:mutant phenotype:GO:0016239
positive regulation of macroautophagy [PMID:27672095]"
/note="Core component of TRAPP complexes I, II and IV;
transport protein particle (TRAPP) complexes are related
multimeric guanine nucleotide-exchange factor for the
GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI),
intra-Golgi traffic (TRAPPII), endosome-Golgi traffic
(TRAPPII and III) and autophagy (TRAPPIII, and IV);
proposed subunit of a novel complex, TRAPPIV, that may
function redundantly with TRAPPIII as a GEF that activates
Ypt1 during autophagy"
/codon_start=1
/product="Trs33p"
/protein_id="NP_014758.3"
/db_xref="GeneID:854282"
/db_xref="SGD:S000005641"
/translation="MSSTHSNNVGHPQSSPQGPLTEQQRAQQQYQIFENSLPKVSQSV
YQMLLNEMVPLAMGIERQISGDVISSDSNVTSENGNINNMIKRLKIEEHHTVDIIRSH
NLIHELYKADEEEKEKVLARLRNIGFQIGLKLSELLIFSNNPNLKFKEMDLLLIMKFI
CRDVWKQIFGKQIDNLKTNHRGTFYLLDYDYRPIQSFSLEEDAKNEELKMIEPFLEIP
VGIIRGVLSSLGYSSEEVICLASFIDRPTDRPKTAFPKGVSFHVQVTMPQ"
gene complement(<539763..>544145)
/gene="RPO31"
/locus_tag="YOR116C"
/gene_synonym="RPC1; RPC160"
/db_xref="GeneID:854283"
mRNA complement(<539763..>544145)
/gene="RPO31"
/locus_tag="YOR116C"
/gene_synonym="RPC1; RPC160"
/product="DNA-directed RNA polymerase III core subunit
RPO31"
/transcript_id="NM_001183535.1"
/db_xref="GeneID:854283"
CDS complement(539763..544145)
/gene="RPO31"
/locus_tag="YOR116C"
/gene_synonym="RPC1; RPC160"
/EC_number="2.7.7.6"
/experiment="EXISTENCE:direct assay:GO:0003899
DNA-directed RNA polymerase activity [PMID:3905793]"
/experiment="EXISTENCE:direct assay:GO:0005666 RNA
polymerase III complex [PMID:10611227|PMID:3905793]"
/experiment="EXISTENCE:direct assay:GO:0042797 tRNA
transcription by RNA polymerase III [PMID:3905793]"
/note="RNA polymerase III largest subunit C160; part of
core enzyme; similar to bacterial beta-prime subunit and
to RPA190 and RPO21"
/codon_start=1
/product="DNA-directed RNA polymerase III core subunit
RPO31"
/protein_id="NP_014759.1"
/db_xref="GeneID:854283"
/db_xref="SGD:S000005642"
/translation="MKEVVVSETPKRIKGLEFSALSAADIVAQSEVEVSTRDLFDLEK
DRAPKANGALDPKMGVSSSSLECATCHGNLASCHGHFGHLKLALPVFHIGYFKATIQI
LQGICKNCSAILLSETDKRQFLHELRRPGVDNLRRMGILKKILDQCKKQRRCLHCGAL
NGVVKKAAAGAGSAALKIIHDTFRWVGKKSAPEKDIWVGEWKEVLAHNPELERYVKRC
MDDLNPLKTLNLFKQIKSADCELLGIDATVPSGRPETYIWRYLPAPPVCIRPSVMMQD
SPASNEDDLTVKLTEIVWTSSLIKAGLDKGISINNMMEHWDYLQLTVAMYINSDSVNP
AMLPGSSNGGGKVKPIRGFCQRLKGKQGRFRGNLSGKRVDFSGRTVISPDPNLSIDEV
AVPDRVAKVLTYPEKVTRYNRHKLQELIVNGPNVHPGANYLLKRNEDARRNLRYGDRM
KLAKNLQIGDVVERHLEDGDVVLFNRQPSLHRLSILSHYAKIRPWRTFRLNECVCTPY
NADFDGDEMNLHVPQTEEARAEAINLMGVKNNLLTPKSGEPIIAATQDFITGSYLISH
KDSFYDRATLTQLLSMMSDGIEHFDIPPPAIMKPYYLWTGKQVFSLLIKPNHNSPVVI
NLDAKNKVFVPPKSKSLPNEMSQNDGFVIIRGSQILSGVMDKSVLGDGKKHSVFYTIL
RDYGPQEAANAMNRMAKLCARFLGNRGFSIGINDVTPADDLKQKKEELVEIAYHKCDE
LITLFNKGELETQPGCNEEQTLEAKIGGLLSKVREEVGDVCINELDNWNAPLIMATCG
SKGSTLNVSQMVAVVGQQIISGNRVPDGFQDRSLPHFPKNSKTPQSKGFVRNSFFSGL
SPPEFLFHAISGREGLVDTAVKTAETGYMSRRLMKSLEDLSCQYDNTVRTSANGIVQF
TYGGDGLDPLEMEGNAQPVNFNRSWDHAYNITFNNQDKGLLPYAIMETANEILGPLEE
RLVRYDNSGCLVKREDLNKAEYVDQYDAERDFYHSLREYINGKATALANLRKSRGMLG
LLEPPAKELQGIDPDETVPDNVKTSVSQLYRISEKSVRKFLEIALFKYRKARLEPGTA
IGAIGAQSIGEPGTQMTLKTFHFAGVASMNVTLGVPRIKEIINASKVISTPIINAVLV
NDNDERAARVVKGRVEKTLLSDVAFYVQDVYKDNLSFIQVRIDLGTIDKLQLELTIED
IAVAITRASKLKIQASDVNIIGKDRIAINVFPEGYKAKSISTSAKEPSENDVFYRMQQ
LRRALPDVVVKGLPDISRAVINIRDDGKRELLVEGYGLRDVMCTDGVIGSRTTTNHVL
EVFSVLGIEAARYSIIREINYTMSNHGMSVDPRHIQLLGDVMTYKGEVLGITRFGLSK
MRDSVLQLASFEKTTDHLFDAAFYMKKDAVEGVSECIILGQTMSIGTGSFKVVKGTNI
SEKDLVPKRCLFESLSNEAALKAN"
gene <545029..>546333
/gene="RPT5"
/locus_tag="YOR117W"
/gene_synonym="YTA1"
/db_xref="GeneID:854284"
mRNA <545029..>546333
/gene="RPT5"
/locus_tag="YOR117W"
/gene_synonym="YTA1"
/product="proteasome regulatory particle base subunit
RPT5"
/transcript_id="NM_001183536.3"
/db_xref="GeneID:854284"
CDS 545029..546333
/gene="RPT5"
/locus_tag="YOR117W"
/gene_synonym="YTA1"
/experiment="EXISTENCE:direct assay:GO:0008540 proteasome
regulatory particle, base subcomplex
[PMID:11742986|PMID:9741626]"
/experiment="EXISTENCE:mutant phenotype:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:9724628]"
/experiment="EXISTENCE:mutant phenotype:GO:0045899
positive regulation of RNA polymerase II transcription
preinitiation complex assembly [PMID:19843524]"
/experiment="EXISTENCE:mutant phenotype:GO:0070682
proteasome regulatory particle assembly [PMID:19412160]"
/note="ATPase of the 19S regulatory particle of the 26S
proteasome; one of six ATPases of the regulatory particle;
involved in the degradation of ubiquitinated substrates;
recruited to the GAL1-10 promoter region upon induction of
transcription; similar to human TBP1"
/codon_start=1
/product="proteasome regulatory particle base subunit
RPT5"
/protein_id="NP_014760.3"
/db_xref="GeneID:854284"
/db_xref="SGD:S000005643"
/translation="MATLEELDAQTLPGDDELDQEILNLSTQELQTRAKLLDNEIRIF
RSELQRLSHENNVMLEKIKDNKEKIKNNRQLPYLVANVVEVMDMNEIEDKENSESTTQ
GGNVNLDNTAVGKAAVVKTSSRQTVFLPMVGLVDPDKLKPNDLVGVNKDSYLILDTLP
SEFDSRVKAMEVDEKPTETYSDVGGLDKQIEELVEAIVLPMKRADKFKDMGIRAPKGA
LMYGPPGTGKTLLARACAAQTNATFLKLAAPQLVQMYIGEGAKLVRDAFALAKEKAPT
IIFIDELDAIGTKRFDSEKSGDREVQRTMLELLNQLDGFSSDDRVKVLAATNRVDVLD
PALLRSGRLDRKIEFPLPSEDSRAQILQIHSRKMTTDDDINWQELARSTDEFNGAQLK
AVTVEAGMIALRNGQSSVKHEDFVEGISEVQARKSKSVSFYA"
gene <546857..>548560
/gene="RTC5"
/locus_tag="YOR118W"
/db_xref="GeneID:854285"
mRNA <546857..>548560
/gene="RTC5"
/locus_tag="YOR118W"
/product="Rtc5p"
/transcript_id="NM_001183537.1"
/db_xref="GeneID:854285"
CDS 546857..548560
/gene="RTC5"
/locus_tag="YOR118W"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:38589611]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0032984
protein-containing complex disassembly [PMID:38589611]"
/note="Protein that regulates the vacuolar V-ATPase;
regulates the assembly state of the V-ATPase holoenzyme,
promoting complex disassembly, and counteracting the
function of the RAVE complex; interacts with V-ATPase
subunits; vacuolar membrane localization requires
N-myristoylation and an assembled V-ATPase; GFP-fusion
protein localizes to the cytoplasm; null mutation
suppresses cdc13-1 temperature sensitivity; member of a
conserved family of TLDc domain proteins found in
eukaryotes"
/codon_start=1
/product="Rtc5p"
/protein_id="NP_014761.1"
/db_xref="GeneID:854285"
/db_xref="SGD:S000005644"
/translation="MGQSSSISSSNEEGSSHSKKFTNSKDILAYFNNKAQQQVTIPEL
VSFKGNLQIEDLNTPISHKALCNSLYFPQNHAMIVGIVTNMLRVLSNFPLMKSSYEPI
TGYGLLKCILLLNRARCAKFLKTKSYDQLKLLFISLSLQKTDKEELSEESENDGNKEL
TIKQIITGFDDVDTEMLCIPADFMLQFLTWLLILTVDCPTTNSKLDNTETHDQWGNFK
VSALNLLRTMNPDVVGDIESHSITFQQFSTAIRTVMPNLLKPLENLMEHFFYLQHDLV
DHDTNLSSIQDSKVMTPALLAQLSTGLPKELFIHKLQSLYIGRKSGFSMRSLQAKVFK
WMAPSILVVSGMRITNSEEYAAEKNPRYRHFLEEFPKLKESDQMMDASHLNKRKTTFA
VYIDDPWKVTNKDYFGDLNTRIIEISPRQDIYKVNQKGTIYFNTIGGGIGIGDKQPLI
KPASKRYIPGNVSLTFDSTLEFAVFRNTGYGGSLDPGLLSMERKEENSPYELHFLIQD
VEVWGCGGEKELEEQIKQLEWEEAESKRRQQINLRSLGEDRALLEMAGLVGQHQGGGS
M"
gene complement(<548792..>550246)
/gene="RIO1"
/locus_tag="YOR119C"
/gene_synonym="RRP10"
/db_xref="GeneID:854286"
mRNA complement(<548792..>550246)
/gene="RIO1"
/locus_tag="YOR119C"
/gene_synonym="RRP10"
/product="protein kinase RIO1"
/transcript_id="NM_001183538.3"
/db_xref="GeneID:854286"
CDS complement(548792..550246)
/gene="RIO1"
/locus_tag="YOR119C"
/gene_synonym="RRP10"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:30011030]"
/experiment="EXISTENCE:direct assay:GO:0000462 maturation
of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA,
5.8S rRNA, LSU-rRNA) [PMID:11483523|PMID:12612080]"
/experiment="EXISTENCE:direct assay:GO:0000776 kinetochore
[PMID:30011030]"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:30011030]"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:11972772|PMID:25851096]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:30011030|PMID:12612080]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11483523]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:30011030]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:30011030]"
/experiment="EXISTENCE:direct assay:GO:0007096 regulation
of exit from mitosis [PMID:11972772]"
/experiment="EXISTENCE:direct assay:GO:0030874 nucleolar
chromatin [PMID:25851096]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:37263996]"
/experiment="EXISTENCE:mutant phenotype:GO:0016479
negative regulation of transcription by RNA polymerase I
[PMID:25851096]"
/experiment="EXISTENCE:mutant phenotype:GO:0090234
regulation of kinetochore assembly [PMID:37263996]"
/experiment="EXISTENCE:mutant phenotype:GO:2000234
positive regulation of rRNA processing [PMID:25851096]"
/note="Serine kinase that controls cell cycle progression;
represses rDNA transcription and promotes 20S pre-rRNA
maturation; catalytic activity regulates its association
with pre-40S particles where it prevents premature entry
into translation; downregulates centromeric RNA levels,
limits RNAPII accessibility, stimulates cenRNA degradation
and promotes timely kinetochore assembly;
autophosphorylates and phosphorylates Rpa43p in anaphase
to remove Pol I from rDNA"
/codon_start=1
/product="protein kinase RIO1"
/protein_id="NP_014762.3"
/db_xref="GeneID:854286"
/db_xref="SGD:S000005645"
/translation="MSLEDKFDSLSVSQGASDHINNQLLEKYSHKIKTDELSFSRAKT
SKDKANRATVENVLDPRTMRFLKSMVTRGVIADLNGCLSTGKEANVYHAFAGTGKAPV
IDEETGQYEVLETDGSRAEYAIKIYKTSILVFKDRERYVDGEFRFRNSRSQHNPRKMI
KIWAEKEFRNLKRIYQSGVIPAPKPIEVKNNVLVMEFLSRGNGFASPKLKDYPYKNRD
EIFHYYHTMVAYMRLLYQVCRLVHADLSEYNTIVHDDKLYMIDVSQSVEPEHPMSLDF
LRMDIKNVNLYFEKMGISIFPERVIFQFVISETLEKFKGDYNNISALVAYIASNLPIK
STEQDEAEDEIFRSLHLVRSLGGLEERDFDRYTDGKFDLLKSLIAHDNERNFAASEQF
EFDNADHECSSGTEEFSDDEEDGSSGSEEDDEEEGEYYDDDEPKVLKGKKHEDKDLKK
LRKQEAKDAKREKRKTKVKKHIKKKLVKKTKSKK"
gene <551114..>552052
/gene="GCY1"
/locus_tag="YOR120W"
/db_xref="GeneID:854287"
mRNA <551114..>552052
/gene="GCY1"
/locus_tag="YOR120W"
/product="glycerol 2-dehydrogenase (NADP(+)) GCY1"
/transcript_id="NM_001183539.1"
/db_xref="GeneID:854287"
CDS 551114..552052
/gene="GCY1"
/locus_tag="YOR120W"
/EC_number="1.1.1.156"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:20844764]"
/experiment="EXISTENCE:direct assay:GO:0004032 aldose
reductase (NADPH) activity [PMID:11306085]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008106 alcohol
dehydrogenase (NADP+) activity
[PMID:17140678|PMID:10818358]"
/experiment="EXISTENCE:direct assay:GO:0016491
oxidoreductase activity [PMID:17962934]"
/experiment="EXISTENCE:direct assay:GO:0019568 arabinose
catabolic process [PMID:12271459]"
/experiment="EXISTENCE:direct assay:GO:0042843 D-xylose
catabolic process [PMID:12271459]"
/experiment="EXISTENCE:genetic interaction:GO:0034599
cellular response to oxidative stress [PMID:17919749]"
/experiment="EXISTENCE:genetic interaction:GO:0061610
glycerol to glycerone phosphate metabolic process
[PMID:22979944]"
/experiment="EXISTENCE:mutant phenotype:GO:0047953
glycerol 2-dehydrogenase (NADP+) activity [PMID:22979944]"
/experiment="EXISTENCE:mutant phenotype:GO:0061610
glycerol to glycerone phosphate metabolic process
[PMID:22979944]"
/note="Glycerol dehydrogenase; involved in an alternative
pathway for glycerol catabolism used under microaerobic
conditions; also has mRNA binding activity; member of the
aldo-keto reductase (AKR) family; human homolog AKR1B1 can
complement yeast null mutant; protein abundance increases
in response to DNA replication stress; GCY1 has a paralog,
YPR1, that arose from the whole genome duplication"
/codon_start=1
/product="glycerol 2-dehydrogenase (NADP(+)) GCY1"
/protein_id="NP_014763.1"
/db_xref="GeneID:854287"
/db_xref="SGD:S000005646"
/translation="MPATLHDSTKILSLNTGAQIPQIGLGTWQSKENDAYKAVLTALK
DGYRHIDTAAIYRNEDQVGQAIKDSGVPREEIFVTTKLWCTQHHEPEVALDQSLKRLG
LDYVDLYLMHWPARLDPAYIKNEDILSVPTKKDGSRAVDITNWNFIKTWELMQELPKT
GKTKAVGVSNFSINNLKDLLASQGNKLTPAANQVEIHPLLPQDELINFCKSKGIVVEA
YSPLGSTDAPLLKEPVILEIAKKNNVQPGHVVISWHVQRGYVVLPKSVNPDRIKTNRK
IFTLSTEDFEAINNISKEKGEKRVVHPNWSPFEVFK"
gene complement(<552298..>552887)
/gene="PFY1"
/locus_tag="YOR122C"
/gene_synonym="CLS5; PRF1"
/db_xref="GeneID:854289"
mRNA complement(join(<552298..552665,552875..>552887))
/gene="PFY1"
/locus_tag="YOR122C"
/gene_synonym="CLS5; PRF1"
/product="profilin"
/transcript_id="NM_001183541.3"
/db_xref="GeneID:854289"
CDS complement(join(552298..552665,552875..552887))
/gene="PFY1"
/locus_tag="YOR122C"
/gene_synonym="CLS5; PRF1"
/experiment="EXISTENCE:direct assay:GO:0003785 actin
monomer binding [PMID:10953013]"
/experiment="EXISTENCE:direct assay:GO:0005546
phosphatidylinositol-4,5-bisphosphate binding
[PMID:8247001]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:7592954]"
/experiment="EXISTENCE:direct assay:GO:0070064
proline-rich region binding [PMID:8247001|PMID:9698363]"
/experiment="EXISTENCE:direct assay:GO:0090338 positive
regulation of formin-nucleated actin cable assembly
[PMID:12134165]"
/experiment="EXISTENCE:direct assay:GO:0140311 protein
sequestering activity [PMID:10953013]"
/experiment="EXISTENCE:genetic interaction:GO:0046907
intracellular transport [PMID:9720869]"
/experiment="EXISTENCE:mutant phenotype:GO:0003785 actin
monomer binding [PMID:10953013]"
/experiment="EXISTENCE:mutant phenotype:GO:0005546
phosphatidylinositol-4,5-bisphosphate binding
[PMID:8247001]"
/experiment="EXISTENCE:mutant phenotype:GO:0070064
proline-rich region binding [PMID:8247001]"
/experiment="EXISTENCE:mutant phenotype:GO:0090338
positive regulation of formin-nucleated actin cable
assembly [PMID:12134165]"
/experiment="EXISTENCE:mutant phenotype:GO:1903475 mitotic
actomyosin contractile ring assembly [PMID:12419188]"
/note="Profilin; binds actin, phosphatidylinositol
4,5-bisphosphate, and polyproline regions; involved in
cytoskeleton organization; required for normal timing of
actin polymerization in response to thermal stress;
protein abundance increases in response to DNA replication
stress; highly conserved protein; human PFN1 (profilin 1)
complements temperature sensitive pfy1 mutants, PFN1
mutations are a rare cause of ALS"
/codon_start=1
/product="profilin"
/protein_id="NP_014765.3"
/db_xref="GeneID:854289"
/db_xref="SGD:S000005648"
/translation="MSWQAYTDNLIGTGKVDKAVIYSRAGDAVWATSGGLSLQPNEIG
EIVQGFDNPAGLQSNGLHIQGQKFMLLRADDRSIYGRHDAEGVVCVRTKQTVIIAHYP
PTVQAGEATKIVEQLADYLIGVQY"
gene complement(<553176..>554570)
/gene="LEO1"
/locus_tag="YOR123C"
/db_xref="GeneID:854290"
mRNA complement(<553176..>554570)
/gene="LEO1"
/locus_tag="YOR123C"
/product="Paf1-complex subunit LEO1"
/transcript_id="NM_001183542.1"
/db_xref="GeneID:854290"
CDS complement(553176..554570)
/gene="LEO1"
/locus_tag="YOR123C"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:20732871]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:15643076]"
/experiment="EXISTENCE:direct assay:GO:1990269 RNA
polymerase II C-terminal domain phosphoserine binding
[PMID:22796944]"
/experiment="EXISTENCE:direct assay:GO:2001209 positive
regulation of transcription elongation by RNA polymerase I
[PMID:20299458]"
/experiment="EXISTENCE:genetic interaction:GO:0006368
transcription elongation by RNA polymerase II
[PMID:11927560]"
/experiment="EXISTENCE:genetic interaction:GO:0090262
regulation of transcription-coupled nucleotide-excision
repair [PMID:21737840]"
/experiment="EXISTENCE:mutant phenotype:GO:0006325
chromatin organization [PMID:20732871]"
/experiment="EXISTENCE:mutant phenotype:GO:0006353
DNA-templated transcription termination [PMID:23109428]"
/experiment="EXISTENCE:mutant phenotype:GO:0009302
sno(s)RNA transcription [PMID:23109428]"
/experiment="EXISTENCE:mutant phenotype:GO:0032968
positive regulation of transcription elongation by RNA
polymerase II [PMID:14710186]"
/experiment="EXISTENCE:physical interaction:GO:0003712
transcription coregulator activity [PMID:11927560]"
/experiment="EXISTENCE:physical interaction:GO:0016593
Cdc73/Paf1 complex [PMID:11927560|PMID:11884586]"
/experiment="EXISTENCE:physical interaction:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:11927560]"
/note="Component of the Paf1 complex; which associates
with RNA polymerase II and is involved in histone
methylation; plays a role in regulating Ty1 transposition;
involved in transcription elongation as demonstrated by
the G-less-based run-on (GLRO) assay"
/codon_start=1
/product="Paf1-complex subunit LEO1"
/protein_id="NP_014766.1"
/db_xref="GeneID:854290"
/db_xref="SGD:S000005649"
/translation="MSSESPQDQPQKEQISNNVGVTTNSTSNEETSRSQDDNVKEVNG
NDDTKEEEQEEDAELDDLFGDDNDDDDDDDVKKSETEKSDSDSDEDDEGENINHRSRH
RESLGLDDDEAEEQAMYTRKFYGEDANNFSDQDETTHTFKEENVELVRHIIPSKANVN
ETASHNEIFYARIPNFLTIDPIPFDPPSFEAKVNERASNSASREDQLDDRLIDENTVR
WRYSRDKDQHVFKESNTQIVQWSDGTYSLKVGEECTDILVNDTSNTFLTVSHDQQELI
QCYEGGEIKKTLMFIPTSTNSKIHQKLSKAVIRRNQRQSKGPGTYIVSMDPEVEKKEL
ERKQSQILRDRRRRQLKEKEKQESPDAAFETGFRKQNSPTTYGASRRNEYEEDDFLVD
DDEEEEAAFDDEEDDNEEEEEEEDADEENASRLRNLKREGAAMYREEEEEEKDRSETK
RRRVAVIEDDEDED"
gene complement(<554824..>558642)
/gene="UBP2"
/locus_tag="YOR124C"
/db_xref="GeneID:854291"
mRNA complement(<554824..>558642)
/gene="UBP2"
/locus_tag="YOR124C"
/product="ubiquitin-specific protease UBP2"
/transcript_id="NM_001183543.3"
/db_xref="GeneID:854291"
CDS complement(554824..558642)
/gene="UBP2"
/locus_tag="YOR124C"
/EC_number="3.4.19.12"
/experiment="EXISTENCE:curator inference:GO:0005737
cytoplasm [PMID:1429680]"
/experiment="EXISTENCE:direct assay:GO:0004843
cysteine-type deubiquitinase activity
[PMID:1429680|PMID:24069405]"
/experiment="EXISTENCE:direct assay:GO:0016579 protein
deubiquitination [PMID:24069405|PMID:15933713]"
/experiment="EXISTENCE:mutant phenotype:GO:0004843
cysteine-type deubiquitinase activity [PMID:24069405]"
/experiment="EXISTENCE:mutant phenotype:GO:0010636
positive regulation of mitochondrial fusion
[PMID:23317502]"
/experiment="EXISTENCE:mutant phenotype:GO:0010992
ubiquitin recycling [PMID:25622294]"
/experiment="EXISTENCE:mutant phenotype:GO:0016579 protein
deubiquitination [PMID:24069405|PMID:23317502]"
/experiment="EXISTENCE:mutant phenotype:GO:0043162
ubiquitin-dependent protein catabolic process via the
multivesicular body sorting pathway [PMID:19165343]"
/experiment="EXISTENCE:mutant phenotype:GO:0070301
cellular response to hydrogen peroxide [PMID:25622294]"
/note="Ubiquitin-specific protease; removes ubiquitin and
polyubiquitin from modified target proteins; controls K63
homeostasis during oxidative stress; deubiquitinates Rsp5p
and is required for MVB sorting of membrane proteins;
removes K63-linked polyubiquitin chains from Rps20p on
free 40S ribosomal subunits for recycling to enhance
ribosome-associated quality control (RQC) functionality"
/codon_start=1
/product="ubiquitin-specific protease UBP2"
/protein_id="NP_014767.3"
/db_xref="GeneID:854291"
/db_xref="SGD:S000005650"
/translation="MPNEDNELQKAIENHHNQLLNQDKENADRNGSVIEDLPLYGTSI
NQQSTPGDVDDGKHLLYPDIATNLPLKTSDRLLDDILCDTIFLNSTDPKVMQKGLQSR
GILKESMLSYSTFRSSIRPNCLGSLTDQVVFQTKSEYDSISCPKYNKIHVFQAVIFNP
SLAEQQISTFDDIVKIPIYHLKVSVKVRQELERLKKHVGVTQFHSLDHLHEYDRVDLS
TFDSSDPNLLDYGIYVSDDTNKLILIEIFKPEFNSPEEHESFTADAIKKRYNAMCVKN
ESLDKSETPSQVDCFYTLFKIFKGPLTRKSKAEPTKTIDSGNLALNTHLNPEWLTSKY
GFQASSEIDEETNEIFTEYVPPDMVDYVNDLETRKIRESFVRKCLQLIFWGQLSTSLL
APNSPLKNTKSVKGMSSLQTSFSTLPWFHLLGESRARILLNSNEQTHSPLDAEPHFIN
LSVSHYYTDRDIIRNYESLSSLDPENIGLYFDALTYIANRKGAYQLIAYCGKQDIIGQ
EALENALLMFKINPKECNISELNEATLLSIYKYETSNKSQVTSNHLTNLKNALRLLAK
YTKSDKLKFYVDHEPYRALSQAYDTLSIDESVDEDIIKTAYSVKINDSPGLKLDCDRA
LYTIAISKRSLDLFNFLTEECPQFSNYYGPEKLDYQEALKLLQVNENASDETILKIFK
QKWFDENVYEPDQFLILRAALTKISIERNSTLITNFLLTGTIDPNSLPPENWPTGINN
IGNTCYLNSLLQYYFSIAPLRRYVLEYQKTVENFNDHLSNSGHIRRIGGREISRGEVE
RSIQFIYQLRNLFYAMVHTRERCVTPSKELAYLAFAPSNVEVEFEVEGNKVVDQTGVL
SDSKKETTDDAFTTKIKDTSLIDLEMEDGLNGDVGTDANRKKNESNDAEVSENEDTTG
LTSPTRVAKISSDQLENALEMGRQQDVTECIGNVLFQIESGSEPIRYDEDNEQYDLVK
QLFYGTTKQSIVPLSATNKVRTKVERFLSLLINIGDHPKDIYDAFDSYFKDEYLTMEE
YGDVIRTVAVTTFPTILQVQIQRVYYDRERLMPFKSIEPLPFKEVIYMDRYADTENPL
LLAKKKETEEMKQKLKVMKNRQRELLSRDDSGLTRKDAFLESIKLLESDTIKKTPLKI
EAANDVIKTLRNNVQNIDNELMKLYNDINSLEEKISHQFDDFKEYGYSLFSVFIHRGE
ASYGHYWIYIKDRNRNGIWRKYNDETISEVQEEEVFNFNEGNTATPYFLVYVKQGQEG
DIEPLKRILK"
gene complement(<559030..>559731)
/gene="CAT5"
/locus_tag="YOR125C"
/gene_synonym="COQ7"
/db_xref="GeneID:854292"
mRNA complement(<559030..>559731)
/gene="CAT5"
/locus_tag="YOR125C"
/gene_synonym="COQ7"
/product="putative monooxygenase CAT5"
/transcript_id="NM_001183544.1"
/db_xref="GeneID:854292"
CDS complement(559030..559731)
/gene="CAT5"
/locus_tag="YOR125C"
/gene_synonym="COQ7"
/EC_number="1.14.99.60"
/EC_number="1.14.13.253"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:9452453]"
/experiment="EXISTENCE:mutant phenotype:GO:0006744
ubiquinone biosynthetic process [PMID:9452453]"
/note="Protein required for ubiquinone (Coenzyme Q)
biosynthesis; localizes to the matrix face of the
mitochondrial inner membrane in a large complex with
ubiquinone biosynthetic enzymes; required for
gluconeogenic gene activation"
/codon_start=1
/product="putative monooxygenase CAT5"
/protein_id="NP_014768.2"
/db_xref="GeneID:854292"
/db_xref="SGD:S000005651"
/translation="MLSRVSVFKPASRGFSVLSSLKITEHTSAKHTEKPEHAPKCQNL
SDAQAAFLDRVIRVDQAGELGADYIYAGQYFVLAHRYPHLKPVLKHIWDQEIHHHNTF
NNLQLKRRVRPSLLTPLWKAGAFAMGAGTALISPEAAMACTEAVETVIGGHYNGQLRN
LANQFNLERTDGTKGPSEEIKSLTSTIQQFRDDELEHLDTAIKHDSYMAVPYTVITEG
IKTICRVAIWSAERI"
gene complement(<559961..>560677)
/gene="IAH1"
/locus_tag="YOR126C"
/db_xref="GeneID:854293"
mRNA complement(<559961..>560677)
/gene="IAH1"
/locus_tag="YOR126C"
/product="isoamyl acetate-hydrolyzing esterase"
/transcript_id="NM_001183545.3"
/db_xref="GeneID:854293"
CDS complement(559961..560677)
/gene="IAH1"
/locus_tag="YOR126C"
/EC_number="3.1.1.112"
/experiment="EXISTENCE:direct assay:GO:0006083 acetate
metabolic process [PMID:10855721]"
/experiment="EXISTENCE:direct assay:GO:0016788 hydrolase
activity, acting on ester bonds [PMID:10855721]"
/note="Isoamyl acetate-hydrolyzing esterase; required in
balance with alcohol acetyltransferase to maintain optimal
amounts of isoamyl acetate, which is particularly
important in sake brewing"
/codon_start=1
/product="isoamyl acetate-hydrolyzing esterase"
/protein_id="NP_014769.3"
/db_xref="GeneID:854293"
/db_xref="SGD:S000005652"
/translation="MDYEKFLLFGDSITEFAFNTRPIEDGKDQYALGAALVNEYTRKM
DILQRGFKGYTSRWALKILPEILKHESNIVMATIFLGANDACSAGPQSVPLPEFIDNI
RQMVSLMKSYHIRPIIIGPGLVDREKWEKEKSEEIALGYFRTNENFAIYSDALAKLAN
EEKVPFVALNKAFQQEGGDAWQQLLTDGLHFSGKGYKIFHDELLKVIETFYPQYHPKN
MQYKLKDWRDVLDDGSNIMS"
gene <561170..>564193
/gene="RGA1"
/locus_tag="YOR127W"
/gene_synonym="DBM1; THE1"
/db_xref="GeneID:854294"
mRNA <561170..>564193
/gene="RGA1"
/locus_tag="YOR127W"
/gene_synonym="DBM1; THE1"
/product="GTPase-activating protein RGA1"
/transcript_id="NM_001183546.1"
/db_xref="GeneID:854294"
CDS 561170..564193
/gene="RGA1"
/locus_tag="YOR127W"
/gene_synonym="DBM1; THE1"
/experiment="EXISTENCE:direct assay:GO:0005096 GTPase
activator activity
[PMID:17981141|PMID:18166650|PMID:7498791|PMID:12455995]"
/experiment="EXISTENCE:direct assay:GO:0005621 cellular
bud scar [PMID:29074565]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0032177 cellular
bud neck split septin rings [PMID:18166650]"
/experiment="EXISTENCE:direct assay:GO:0071597 cellular
birth scar [PMID:29074565]"
/experiment="EXISTENCE:genetic interaction:GO:0000750
pheromone-dependent signal transduction involved in
conjugation with cellular fusion [PMID:7498791]"
/experiment="EXISTENCE:genetic interaction:GO:0005096
GTPase activator activity [PMID:7498791]"
/experiment="EXISTENCE:genetic interaction:GO:0031106
septin ring organization [PMID:14517318]"
/experiment="EXISTENCE:mutant phenotype:GO:0007015 actin
filament organization [PMID:8657111]"
/experiment="EXISTENCE:mutant phenotype:GO:0007120 axial
cellular bud site selection [PMID:29074565]"
/experiment="EXISTENCE:mutant phenotype:GO:0030010
establishment of cell polarity [PMID:18166650]"
/experiment="EXISTENCE:physical interaction:GO:0001403
invasive growth in response to glucose limitation
[PMID:7498791]"
/experiment="EXISTENCE:physical interaction:GO:0007118
budding cell apical bud growth [PMID:7498791]"
/experiment="EXISTENCE:physical interaction:GO:0007119
budding cell isotropic bud growth [PMID:7498791]"
/experiment="EXISTENCE:physical interaction:GO:2000222
positive regulation of pseudohyphal growth [PMID:7498791]"
/note="GTPase-activating protein for polarity-regulator
Cdc42p (RhoGAP); required for proper bud site selection;
transiently localizes to previous cell division sites (bud
scars; cytokinesis remnants), interacting with Nis1p and
Nba1p, to prevent Cdc42p repolarization; implicated in
control of septin organization, pheromone response, and
haploid invasive growth; relocalizes from the bud neck to
cytoplasm upon DNA replication stress; contains N-terminal
LIM domains and a C-terminal GAP domain"
/codon_start=1
/product="GTPase-activating protein RGA1"
/protein_id="NP_014770.1"
/db_xref="GeneID:854294"
/db_xref="SGD:S000005653"
/translation="MASTAPNEQFPSCVRCKEFITTGHAYELGCDRWHTHCFACYKCE
KPLSCESDFLVLGTGALICFDCSDSCKNCGKKIDDLAIILSSSNEAYCSDCFKCCKCG
ENIADLRYAKTKRGLFCLSCHEKLLAKRKYYEEKKRRLKKNLPSLPTPVIDNGHTDEV
SASAVLPEKTFSRPASLVNEIPSGSEPSKDIETNSSDIVPHFITGYNDSDDNSGSSKF
GSNVSIDVIGPEENSTEHVNDDVKEEAEAPSANMSLNVATDPTLSCKEPPSHSRNLLN
KTPLRNSSGQYLAKSPSSYRQGIIVNDSLEESDQIDPPNNSSRNASELLTSVLHSPVS
VNMKNPKGSNTDIFNTGEISQMDPSLSRKVLNNIVEETNALQRPVVEVVKEDRSVPDL
AGVQQEQAEKYSYSNNSGKGRKISRSLSRRSKDLMINLKSRATGKQDSNVKLSPASKV
TSRRSQDLMRDNDSHTGLDTPNSNSTSLDILVNNQKSLNYKRFTDNGTLRVTSGKETA
LEEQKNHSFKSPSPIDHLLQSPATPSNVSMYRTPPLDSSLTFDRRNGSSYSNQNYSIP
SWQKTPKTQLENSDNFEEQKETLYENSESRNDPSLDKEIVTAEHYLKQLKINLKELES
QREELMKEITEMKSMKEALRRHIESYNSEKNKLYLDSNELSNNPPMINEISLGESPPV
KHVATASSVARSSVKPKFWKFFSSAKPQTEQSIQGVSTNNTNSIVKSAPVLLSAPSSG
SNSGRLEISPPVLQNPNEFSDVRLVPIENDANMGQSKDGEEYLDGSNLYGSSLVARCN
YENNEIPMILSVCIDFIESDEENMRSEGIYRKSGSQLVIEEIEKQFSAWKVQQNTETP
NILTEQDLNVVTGVLKRYLRKLPNPIFTFQIYEPLMRLVKSKKMMENLPFVGGKLSLE
AKNSDTYMSSKSALKNILEDLPREHYRVLRVLSEHIEKVTRYSHWNRMTLYNLALVFA
PGLIRDFSGEKDIIDMKERNYIVAFIFGNYKDILT"
gene complement(<564476..>566191)
/gene="ADE2"
/locus_tag="YOR128C"
/db_xref="GeneID:854295"
mRNA complement(<564476..>566191)
/gene="ADE2"
/locus_tag="YOR128C"
/product="phosphoribosylaminoimidazole carboxylase ADE2"
/transcript_id="NM_001183547.3"
/db_xref="GeneID:854295"
CDS complement(564476..566191)
/gene="ADE2"
/locus_tag="YOR128C"
/EC_number="4.1.1.21"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0004638
phosphoribosylaminoimidazole carboxylase activity
[PMID:5767024]"
/experiment="EXISTENCE:mutant phenotype:GO:0006144 purine
nucleobase metabolic process [PMID:8939809]"
/note="Phosphoribosylaminoimidazole carboxylase; catalyzes
a step in the 'de novo' purine nucleotide biosynthetic
pathway; red pigment accumulates in mutant cells deprived
of adenine"
/codon_start=1
/product="phosphoribosylaminoimidazole carboxylase ADE2"
/protein_id="NP_014771.3"
/db_xref="GeneID:854295"
/db_xref="SGD:S000005654"
/translation="MDSRTVGILGGGQLGRMIVEAANRLNIKTVILDAENSPAKQISN
SNDHVNGSFSNPLDIEKLAEKCDVLTIEIEHVDVPTLKNLQVKHPKLKIYPSPETIRL
IQDKYIQKEHLIKNGIAVTQSVPVEQASETSLLNVGRDLGFPFVLKSRTLAYDGRGNF
VVKNKEMIPEALEVLKDRPLYAEKWAPFTKELAVMIVRSVNGLVFSYPIVETIHKDNI
CDLCYAPARVPDSVQLKAKLLAENAIKSFPGCGIFGVEMFYLETGELLINEIAPRPHN
SGHYTIDACVTSQFEAHLRSILDLPMPKNFTSFSTITTNAIMLNVLGDKHTKDKELET
CERALATPGSSVYLYGKESRPNRKVGHINIIASSMAECEQRLNYITGRTDIPIKISVA
QKLDLEAMVKPLVGIIMGSDSDLPVMSAACAVLKDFGVPFEVTIVSAHRTPHRMSAYA
ISASKRGIKTIIAGAGGAAHLPGMVAAMTPLPVIGVPVKGSCLDGVDSLHSIVQMPRG
VPVATVAINNSTNAALLAVRLLGAYDSSYTTKMEQFLLKQEEEVLVKAQKLETVGYEA
YLENK"
rep_origin 566410..566643
/note="ARS1516; Autonomously Replicating Sequence"
/db_xref="SGD:S000114843"
gene complement(<566877..>569558)
/gene="AFI1"
/locus_tag="YOR129C"
/db_xref="GeneID:854296"
mRNA complement(<566877..>569558)
/gene="AFI1"
/locus_tag="YOR129C"
/product="Afi1p"
/transcript_id="NM_001183548.1"
/db_xref="GeneID:854296"
CDS complement(566877..569558)
/gene="AFI1"
/locus_tag="YOR129C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:18397879]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:18397879]"
/experiment="EXISTENCE:genetic interaction:GO:0051666
actin cortical patch localization [PMID:18397879]"
/experiment="EXISTENCE:mutant phenotype:GO:0000282
cellular bud site selection [PMID:18397879]"
/experiment="EXISTENCE:mutant phenotype:GO:0005634 nucleus
[PMID:14515169]"
/experiment="EXISTENCE:mutant phenotype:GO:0005886 plasma
membrane [PMID:18397879|PMID:14515169]"
/experiment="EXISTENCE:mutant phenotype:GO:0051666 actin
cortical patch localization [PMID:18397879]"
/note="Arf3p polarization-specific docking factor;
required for the polarized distribution of the
ADP-ribosylation factor, Arf3p; participates in polarity
development and maintenance of a normal haploid budding
pattern; interacts with Cnm7p"
/codon_start=1
/product="Afi1p"
/protein_id="NP_014772.1"
/db_xref="GeneID:854296"
/db_xref="SGD:S000005655"
/translation="MLRRELNNSISNRSIENESFPFERPNVSYIISAEFDNKLGPILK
HQYPKDIPGFNQFSHEQRNGNTSVSMNLASLMIPSSIERNPGKQDITVFTLYYNKFTQ
NYQLFPVPKDPRFSFNLHHREQSDGSVTNSIYYDAENHQDAKNNRYTIVLEDDELECQ
EVQNNQKAIDNEPLFFINVANTVLDTTNDRGAVIKSIAIGTPLKTFFAFKNIIVLVLD
LYMKAPTQAAATDILLDCFNMLNSIDLTLINDIHSKSSIQEVLHSIHDESIITKVFLD
PDSTLKKLFCINGFDTKDKYGNIVTFHDQLIQYHFTRFQPKTLPPFLLKIPLQFNMIR
REPIYIENDYNELVLKFLDKFVPYLLKAGQKVNAWKLVINSTKLSKEDLCAFILSLAN
ITATYASDPQSYFKGNAALIFPYMDISLVDGLRAYVASNSDFVGCFAIIGTANPIFRY
QLDIWDYYYDVDEGVFYENNSPEKEKPDTVAEVKIGPNPLRKIFNRPHFSTNAVNESQ
VNLGQKLFSLLIDEYHDSDTIMSVLRRLNVLQLENLLDALKRREIPPNIALKDEYIMF
YKDFFIFPEFFDYFTLHSIELLSNLDNCLFSLGNTCQLFSTEQIYSQLSQILDIVKEL
FRMVSVSRTNIEKFLNACLNYSPFKILPTAQLHGDNISRWSFESEVRQGFDNFNSYMG
IEKDPHGVIVSAIDLFTQIYSFDILAFFLTFITKESGQDLPFTKSLSRRRTYLTRIAQ
SSSLRQFLQLSTRPNIRILGGNGQGTGNSNYPEFTNASSVISPKLRASPLLERRASKI
CYAITKLLYRLECHPIGMALLKKYLHNQLREAYLESKRHFISKKGDSTNTSSTIASSS
FAGASVPLSSNESGMLNGLKQINEQQESTLETTQKED"
gene complement(<569929..>570807)
/gene="ORT1"
/locus_tag="YOR130C"
/gene_synonym="ARG11"
/db_xref="GeneID:854297"
mRNA complement(<569929..>570807)
/gene="ORT1"
/locus_tag="YOR130C"
/gene_synonym="ARG11"
/product="Ort1p"
/transcript_id="NM_001183549.4"
/db_xref="GeneID:854297"
CDS complement(569929..570807)
/gene="ORT1"
/locus_tag="YOR130C"
/gene_synonym="ARG11"
/experiment="EXISTENCE:direct assay:GO:0000064 L-ornithine
transmembrane transporter activity [PMID:9237680]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005740
mitochondrial envelope [PMID:8798783]"
/experiment="EXISTENCE:direct assay:GO:1990575
mitochondrial L-ornithine transmembrane transport
[PMID:9237680]"
/experiment="EXISTENCE:mutant phenotype:GO:0006526
L-arginine biosynthetic process [PMID:8798783]"
/note="Ornithine transporter of the mitochondrial inner
membrane; exports ornithine from mitochondria as part of
arginine biosynthesis; functionally complemented by human
ortholog, SLC25A15, which is associated with
hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH)
syndrome, but HHH-associated variants fail to complement"
/codon_start=1
/product="Ort1p"
/protein_id="NP_014773.4"
/db_xref="GeneID:854297"
/db_xref="SGD:S000005656"
/translation="MEDSKKKGLIEGAILDIINGSIAGACGKVIEFPFDTVKVRLQTQ
ASNVFPTTWSCIKFTYQNEGIARGFFQGIASPLVGACLENATLFVSYNQCSKFLEKHT
NVSPLGQILISGGVAGSCASLVLTPVELVKCKLQVANLQVASAKTKHTKVLPTIKAII
TERGLAGLWQGQSGTFIRESFGGVAWFATYEIVKKSLKDRHSLDDPKRDESKIWELLI
SGGSAGLAFNASIFPADTVKSVMQTEHISLTNAVKKIFGKFGLKGFYRGLGITLFRAV
PANAAVFYIFETLSAL"
gene 571958..572029
/locus_tag="YNCO0019W"
/db_xref="GeneID:854298"
tRNA 571958..572029
/locus_tag="YNCO0019W"
/product="tRNA-Asp"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:854298"
/db_xref="SGD:S000006545"
gene complement(<572182..>572838)
/locus_tag="YOR131C"
/db_xref="GeneID:854299"
mRNA complement(<572182..>572838)
/locus_tag="YOR131C"
/product="putative haloacid dehalogenase-like hydrolase"
/transcript_id="NM_001183550.1"
/db_xref="GeneID:854299"
CDS complement(572182..572838)
/locus_tag="YOR131C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0033883 pyridoxal
phosphatase activity [PMID:26071590]"
/note="Putative haloacid dehalogenase-like hydrolase;
non-essential gene; overexpression causes a cell cycle
delay or arrest; protein abundance increases in response
to DNA replication stress"
/codon_start=1
/product="putative haloacid dehalogenase-like hydrolase"
/protein_id="NP_014774.1"
/db_xref="GeneID:854299"
/db_xref="SGD:S000005657"
/translation="MTKLQGLQGLKHIKAVVFDMDGTLCLPQPWMFPAMRNAIGLEDK
SIDILHFIDTLPTEKEKKEAHDRIELVEAKAMKEMQPQPGLVDIMRYLTKNGISKNIC
TRNVGAPVETFVKRFIPSELSRFDYIVTREFRPTKPQPDPLLHIASKLNIRPLEMIMV
GDSFDDMKSGRSAGCFTVLLKNHVNGHLLLEHKELVDVSVEDLSEIIELIQNMNKESF
"
gene <573175..>574830
/gene="VPS17"
/locus_tag="YOR132W"
/gene_synonym="PEP21; VPT3"
/db_xref="GeneID:854300"
mRNA <573175..>574830
/gene="VPS17"
/locus_tag="YOR132W"
/gene_synonym="PEP21; VPT3"
/product="retromer subunit VPS17"
/transcript_id="NM_001183551.3"
/db_xref="GeneID:854300"
CDS 573175..574830
/gene="VPS17"
/locus_tag="YOR132W"
/gene_synonym="PEP21; VPT3"
/experiment="EXISTENCE:direct assay:GO:0032266
phosphatidylinositol-3-phosphate binding [PMID:11557775]"
/experiment="EXISTENCE:mutant phenotype:GO:0030904
retromer complex [PMID:9700157]"
/experiment="EXISTENCE:mutant phenotype:GO:0140318 protein
transporter activity [PMID:9700157]"
/experiment="EXISTENCE:physical interaction:GO:0005768
endosome [PMID:9700157]"
/experiment="EXISTENCE:physical interaction:GO:0030904
retromer complex [PMID:9700157]"
/experiment="EXISTENCE:physical interaction:GO:0030905
retromer, tubulation complex [PMID:9700157]"
/experiment="EXISTENCE:physical interaction:GO:0042147
retrograde transport, endosome to Golgi [PMID:9700157]"
/experiment="EXISTENCE:physical interaction:GO:0140318
protein transporter activity [PMID:9700157]"
/note="Subunit of the membrane-associated retromer
complex; essential for endosome-to-Golgi retrograde
protein transport; peripheral membrane protein that
assembles onto the membrane with Vps5p to promote vesicle
formation; required for recruiting the retromer complex to
the endosome membranes"
/codon_start=1
/product="retromer subunit VPS17"
/protein_id="NP_014775.3"
/db_xref="GeneID:854300"
/db_xref="SGD:S000005658"
/translation="MTSAVPYDPYDDLDNNPFAEPQEEDSEPAATTTDGSSSMSEERV
GTEQTAASVQDNGTANNIQNGLGEEGNATRSKTSNEHNENQQPSQPSERVILPERSDE
KKKYTLLAKVTGLERFGSATGKKENPTIIFDCSTNLPTFRKQQYKNVKKSYEEFHQLF
KYLNVAIQESFVPTLPSAYTTFGINSEEDRMKVTRNFQLWFNRLSQDPLIIRNEEVAF
FIESDFNTYTPINKSKSLASGLKRKTLKQLAPPYDEITELAEFRPLVKSIYVVSQSLQ
EKLLRVSRNRKMMVQEENAFGQDFVNLDEHNKLYRRYGKILTAVGDIDSIIATMDMAT
LYDGLEWIVRDAYAVKEALTNRHFIMRNLVQAQQNSKAKQEQARRFRSRRDINPMKID
EALRQLKAAAKNEQVLTLKLQRITSNMIIERKQWISWYEEWIRSSIKEFTLRKIEYER
KKLTLLERVRSDIRKADENGGLSRLGRHAVSNNNSDTSQTLKGDSWTGESNRKSQIPI
NKIAHTEFDDELFTEDDGYNSQDSDTTSLNARHAASLLGMSTK"
gene <575098..>577626
/gene="EFT1"
/locus_tag="YOR133W"
/db_xref="GeneID:854301"
mRNA <575098..>577626
/gene="EFT1"
/locus_tag="YOR133W"
/product="elongation factor 2"
/transcript_id="NM_001183552.1"
/db_xref="GeneID:854301"
CDS 575098..577626
/gene="EFT1"
/locus_tag="YOR133W"
/experiment="EXISTENCE:direct assay:GO:0003924 GTPase
activity [PMID:16950777]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:16950777|PMID:10835368]"
/experiment="EXISTENCE:direct assay:GO:0051087
protein-folding chaperone binding [PMID:30876804]"
/experiment="EXISTENCE:mutant phenotype:GO:0006414
translational elongation [PMID:16950777]"
/experiment="EXISTENCE:mutant phenotype:GO:0045901
positive regulation of translational elongation
[PMID:19338753]"
/experiment="EXISTENCE:mutant phenotype:GO:1990145
maintenance of translational fidelity [PMID:16950777]"
/note="Elongation factor 2 (eEF2, EF-2), also encoded by
EFT2; catalyzes ribosomal translocation during protein
synthesis; contains diphthamide, the unique
posttranslationally modified histidine residue
specifically ADP-ribosylated by diphtheria toxin; EFT1 has
a paralog, EFT2, that arose from the whole genome
duplication"
/codon_start=1
/product="elongation factor 2"
/protein_id="NP_014776.1"
/db_xref="GeneID:854301"
/db_xref="SGD:S000005659"
/translation="MVAFTVDQMRSLMDKVTNVRNMSVIAHVDHGKSTLTDSLVQRAG
IISAAKAGEARFTDTRKDEQERGITIKSTAISLYSEMSDEDVKEIKQKTDGNSFLINL
IDSPGHVDFSSEVTAALRVTDGALVVVDTIEGVCVQTETVLRQALGERIKPVVVINKV
DRALLELQVSKEDLYQTFARTVESVNVIVSTYADEVLGDVQVYPARGTVAFGSGLHGW
AFTIRQFATRYAKKFGVDKAKMMDRLWGDSFFNPKTKKWTNKDTDAEGKPLERAFNMF
ILDPIFRLFTAIMNFKKDEIPVLLEKLEIVLKGDEKDLEGKALLKVVMRKFLPAADAL
LEMIVLHLPSPVTAQAYRAEQLYEGPADDANCIAIKNCDPKADLMLYVSKMVPTSDKG
RFYAFGRVFAGTVKSGQKVRIQGPNYVPGKKDDLFIKAIQRVVLMMGRFVEPIDDCPA
GNIIGLVGIDQFLLKTGTLTTSETAHNMKVMKFSVSPVVQVAVEVKNANDLPKLVEGL
KRLSKSDPCVLTYMSESGEHIVAGTGELHLEICLQDLEHDHAGVPLKISPPVVAYRET
VESESSQTALSKSPNKHNRIYLKAEPIDEEVSLAIENGIINPRDDFKARARIMADDYG
WDVTDARKIWCFGPDGNGPNLVIDQTKAVQYLHEIKDSVVAAFQWATKEGPIFGEEMR
SVRVNILDVTLHADAIHRGGGQIIPTMRRATYAGFLLADPKIQEPVFLVEIQCPEQAV
GGIYSVLNKKRGQVVSEEQRPGTPLFTVKAYLPVNESFGFTGELRQATGGQAFPQMVF
DHWSTLGSDPLDPTSKAGEIVLAARKRHGMKEEVPGWQEYYDKL"
gene <578564..>579793
/gene="BAG7"
/locus_tag="YOR134W"
/db_xref="GeneID:854302"
mRNA <578564..>579793
/gene="BAG7"
/locus_tag="YOR134W"
/product="GTPase-activating protein BAG7"
/transcript_id="NM_001183553.3"
/db_xref="GeneID:854302"
CDS 578564..579793
/gene="BAG7"
/locus_tag="YOR134W"
/experiment="EXISTENCE:direct assay:GO:0005096 GTPase
activator activity [PMID:12207708|PMID:11591390]"
/experiment="EXISTENCE:genetic interaction:GO:0035024
negative regulation of Rho protein signal transduction
[PMID:12207708]"
/note="Rho GTPase activating protein (RhoGAP); stimulates
the intrinsic GTPase activity of Rho1p, which plays a bud
growth by regulating actin cytoskeleton organization and
cell wall biosynthesis, resulting in the downregulation of
Rho1p; structurally and functionally related to Sac7p;
BAG7 has a paralog, SAC7, that arose from the whole genome
duplication"
/codon_start=1
/product="GTPase-activating protein BAG7"
/protein_id="NP_014777.3"
/db_xref="GeneID:854302"
/db_xref="SGD:S000005660"
/translation="MFNMNLLSTPSSEEGSPQNRSSSMSSVEGKKDRDTFTNLQNEFD
GKVFGVSLEESLKVAQEEVIIQKSTNEIGSIPVVIAKSGKYLKENALDTTGIFRIAGS
NKRVRELQAVFSKPPDYGRKFEGWCDFNVHDIATLLKRYLNSLSEPLVPLALYDIFRN
PILENPKINEHKEQIIKDYEDIYMLLPQQNRHLILYLAALLNLFARNEKKNLMSASNL
AAIVQPSLLSHPKDEMCPKEYEASRTVIEFLILHASDIIPNTEKANKDTMPHAGTVAK
FNNITVPEMAIDSDEEDFVHPSIDDHMLPRSRALSDSNNFTIHHHHHHHHALFPSPID
FDNNGLSVPRSFKGRTLSAESLSPRLSKLLGNVGNSSNTGIKDPTERVPRGEHKTKHK
QRQSWLRRLTSPSRTQP"
gene <580250..>581359
/gene="IDH2"
/locus_tag="YOR136W"
/db_xref="GeneID:854303"
mRNA <580250..>581359
/gene="IDH2"
/locus_tag="YOR136W"
/product="isocitrate dehydrogenase (NAD(+)) IDH2"
/transcript_id="NM_001183555.1"
/db_xref="GeneID:854303"
CDS 580250..581359
/gene="IDH2"
/locus_tag="YOR136W"
/EC_number="1.1.1.41"
/experiment="EXISTENCE:direct assay:GO:0004449 isocitrate
dehydrogenase (NAD+) activity [PMID:16884682]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:11914276|PMID:16823961|PMID:14576278|PMID:24769239|P
MID:11502169]"
/experiment="EXISTENCE:direct assay:GO:0006102 isocitrate
metabolic process [PMID:16884682]"
/experiment="EXISTENCE:direct assay:GO:0045242 isocitrate
dehydrogenase complex (NAD+) [PMID:16884682]"
/note="Subunit of mitochondrial NAD(+)-dependent
isocitrate dehydrogenase; complex catalyzes oxidation of
isocitrate to alpha-ketoglutarate in TCA cycle; targeted
to vacuole via AP-3 pathway"
/codon_start=1
/product="isocitrate dehydrogenase (NAD(+)) IDH2"
/protein_id="NP_014779.1"
/db_xref="GeneID:854303"
/db_xref="SGD:S000005662"
/translation="MLRNTFFRNTSRRFLATVKQPSIGRYTGKPNPSTGKYTVSFIEG
DGIGPEISKSVKKIFSAANVPIEWESCDVSPIFVNGLTTIPDPAVQSITKNLVALKGP
LATPIGKGHRSLNLTLRKTFGLFANVRPAKSIEGFKTTYENVDLVLIRENTEGEYSGI
EHIVCPGVVQSIKLITRDASERVIRYAFEYARAIGRPRVIVVHKSTIQRLADGLFVNV
AKELSKEYPDLTLETELIDNSVLKVVTNPSAYTDAVSVCPNLYGDILSDLNSGLSAGS
LGLTPSANIGHKISIFEAVHGSAPDIAGQDKANPTALLLSSVMMLNHMGLTNHADQIQ
NAVLSTIASGPENRTGDLAGTATTSSFTEAVIKRL"
gene complement(<581813..>583681)
/gene="SIA1"
/locus_tag="YOR137C"
/db_xref="GeneID:854305"
mRNA complement(<581813..>583681)
/gene="SIA1"
/locus_tag="YOR137C"
/product="Sia1p"
/transcript_id="NM_001183556.3"
/db_xref="GeneID:854305"
CDS complement(581813..583681)
/gene="SIA1"
/locus_tag="YOR137C"
/experiment="EXISTENCE:mutant phenotype:GO:1902600 proton
transmembrane transport [PMID:9450541]"
/note="Protein of unassigned function; involved in
activation of the Pma1p plasma membrane H+-ATPase by
glucose; contains peptide signal for membrane
localization"
/codon_start=1
/product="Sia1p"
/protein_id="NP_014780.3"
/db_xref="GeneID:854305"
/db_xref="SGD:S000005663"
/translation="MRLHYRRRFNFLRRILFILCITSLYLSRDSLKLHAKNVLMDHNV
AEYHGGMIDDIQILRCYHWYRQCSSLYAPKLHPSNTAKKIKDKNSILWTRVSKNITVE
TLYSLQSGPFYNSYLYVHLKDFQSNPKNTIKELAIARDSALIPLQVLRDINKLVKSSD
SSVFHNHVYLREKPTSSWWKLLFGISVDTDNIAVFGEEWVYKGSGIWCKYILNDDDND
APITNLEIYLGSSFIESRPSWKEVIHEFHRNNIPSLPISITRKLETKNHHHKFSNGLL
GSLRTPSKDINIQVDADYKITSPHIQFSRGQRSFKILQITDFHFKCTDNSMTVINEIK
TVNFIDRVLASENPDLVVITGDLLDSHNTIDYQTCIMKVVQPMISNKIPYAISLGVSD
ESNLATSAQIRDFIRNLPYTFNNVASEEGHMAIEVSFKKKLTKNTLLERDIDTEDETN
PSEALFFVFDSFAPVNNFLQDYNDLIGKIDFGLAFQYFPLSEYRPHGLFPIIGQYNER
STLTVDTPRSRGQVSMTINGKHYKSFLDILSLWNIKGVSCGHEHNNDCCLQSKNEMWL
CYGGSAGIGLPRIQGIYPTVRLFNLDDILDEITSWKRNSNLVDEVYDYQYIYKGKQ"
gene complement(<584309..>586324)
/gene="RUP1"
/locus_tag="YOR138C"
/db_xref="GeneID:854306"
mRNA complement(<584309..>586324)
/gene="RUP1"
/locus_tag="YOR138C"
/product="Rup1p"
/transcript_id="NM_001183557.1"
/db_xref="GeneID:854306"
CDS complement(584309..586324)
/gene="RUP1"
/locus_tag="YOR138C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:1902499 positive
regulation of protein autoubiquitination [PMID:24069405]"
/experiment="EXISTENCE:mutant phenotype:GO:0016579 protein
deubiquitination [PMID:15933713]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:15933713]"
/note="Protein that regulates ubiquitination of Rsp5p; has
a WW domain consensus motif of PPPSY (residues 131-135)
that mediates binding of Rsp5p to Ubp2p; contains an UBA
domain; relative distribution to the nucleus increases
upon DNA replication stress"
/codon_start=1
/product="Rup1p"
/protein_id="NP_014781.1"
/db_xref="GeneID:854306"
/db_xref="SGD:S000005664"
/translation="MMDNQAVKSLLEMGIPHEVAVDALQRTGGNLEAAVNFIFSNELP
EQAEMGEENDGSQPRISENKIVAGTKPCDVPNNGDQDIDMPDVSGVDVDYDDDEDITD
ERSGSNSTSGCRVTAQNYDRYSISETSIPPPSYSIVQHNEFKSNVGDPTVVLPLPLNS
LIESYFGLFALLTAVYFPHVFLKPDFKDLNYRADWFKGSSFTEPKYRLAYCEAEDGST
TSEIVLASGPNEGLQPHLLWQLQKLISVVNTRKCERAFVSAKVFTSSLEPQLRSKLAD
SEHLYEVLPAFIKSLAVDLEMCPGIRDRETRSLFISSALHTPNKNEPPMETFLSLFHF
LPEEYDSNLYKMFNVLLYPEEEEEEEDVIRGGEQEEARYVEPENTLKEVAPVLTILFN
ELETNTESVSLPNGVDIPLEFYPQLYTKQCKDQLIRHIISKRKQARTRSRCLLQEINE
LKSYQGKNISTILESTLAYLQTIPDDANNEAAKQIASLKDTLNSARAAKMEEYKDLAS
KLHGEWNLSHPETHIINTAKQLGLIENPYILTMAALSPYSYFIRSRNGAWSWIQSNTL
GTEFKVKKCSSPSVVQEAIKHGTKYASETPLMFIYCEEGKIPTEEVVAEALKSNSGCL
KFAEDDQNSLKTLRSQFFDGMGDPEQATNNINNGNDNDNDDDIDSDN"
gene <586981..>589281
/gene="SFL1"
/locus_tag="YOR140W"
/db_xref="GeneID:854307"
mRNA <586981..>589281
/gene="SFL1"
/locus_tag="YOR140W"
/product="Sfl1p"
/transcript_id="NM_001183559.4"
/db_xref="GeneID:854307"
CDS 586981..589281
/gene="SFL1"
/locus_tag="YOR140W"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:11399075|PMID:12024012|PMID:9755175]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12024012]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:27336723]"
/experiment="EXISTENCE:genetic interaction:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:9811878]"
/experiment="EXISTENCE:genetic interaction:GO:2000218
negative regulation of invasive growth in response to
glucose limitation [PMID:9811878]"
/experiment="EXISTENCE:genetic interaction:GO:2000221
negative regulation of pseudohyphal growth [PMID:9811878]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:9811878|PMID:12024012|PMID:15466424]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:17594096|PMID:27336723]"
/experiment="EXISTENCE:mutant phenotype:GO:2000218
negative regulation of invasive growth in response to
glucose limitation [PMID:15466424|PMID:9811878]"
/experiment="EXISTENCE:mutant phenotype:GO:2000221
negative regulation of pseudohyphal growth
[PMID:12024012|PMID:9811878]"
/experiment="EXISTENCE:physical interaction:GO:0000981
DNA-binding transcription factor activity, RNA polymerase
II-specific [PMID:9755175|PMID:11399075]"
/experiment="EXISTENCE:physical interaction:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:11399075]"
/note="Transcriptional repressor and activator; involved
in repression of flocculation-related genes, and
activation of stress responsive genes; has direct role in
INO1 transcriptional memory; negatively regulated by
cAMP-dependent protein kinase A subunit Tpk2p; premature
stop codon (C1430T, Q477-stop) in SK1 background is linked
to the aggressively invasive phenotype of SK1 relative to
BY4741 (S288C)"
/codon_start=1
/product="Sfl1p"
/protein_id="NP_014783.4"
/db_xref="GeneID:854307"
/db_xref="SGD:S000005666"
/translation="MSEEETVSAPAPASTPAPAGTDVGSGGAAAGIANAGAEGGDGAE
DVKKHGSKMLVGPRPPQNAIFIHKLYQILEDESLHDLIWWTPSGLSFMIKPVERFSKA
LATYFKHTNITSFVRQLNIYGFHKVSHDHSSNDANSGDDANTNDDSNTHDDNSGNKNS
SGDENTGGGVQEKEKSNPTKIWEFKHSSGIFKKGDIEGLKHIKRRASSRNNSSINSRK
NSSNQNYDIDSGARVRPSSIQDPSTSSNSFGNFVPQIPGANNSIPEYFNNSHVTYENA
NHAPLESNNPEMQEQNRPPNFQDETLKHLKEINFDMVKIIESMQHFISLQHSFCSQSF
TFKNVSKKKSENIVKDHQKQLQAFESDMLTFKQHVMSRAHRTIDSLCAVNAAATAASV
APAPAPTSTSAYAPKSQYEMMVPPGNQYVPQKSSSTTNIPSRFNTASVPPSQLFVQYQ
PQSQQHVTYAKQPAHVPNFINQPIPIQQLPPQYADTFSTPQMMHNPFASKNNNKPGNT
KRTNSVLMDPLTPAASVGVQGPLNYPIMNINPSVRDYNKPVPQNMAPSPIYPINEPTT
RLYSQPKMRSLGSTSSLPNDRRNSPLKLTPRSSLNEDSLYPKPRNSLKSSISGTSLSS
SFTLVANNPAPIRYSQQGLLRSLNKAANCAPDSVTPLDSSVLTGPPPKNMDNLPAVSS
NLINSPMNVEHSSSLSQAEPAPQIELPQPSLPTTSTTKNTGEADNSKRKGSGVYSLLN
QEDSSTSSADPKTEDKAAPALKKVKM"
gene complement(<589942..>592587)
/gene="ARP8"
/locus_tag="YOR141C"
/db_xref="GeneID:854309"
mRNA complement(<589942..>592587)
/gene="ARP8"
/locus_tag="YOR141C"
/product="Arp8p"
/transcript_id="NM_001183560.1"
/db_xref="GeneID:854309"
CDS complement(589942..592587)
/gene="ARP8"
/locus_tag="YOR141C"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:20844764]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11011149]"
/experiment="EXISTENCE:mutant phenotype:GO:0006302
double-strand break repair [PMID:23644470]"
/experiment="EXISTENCE:mutant phenotype:GO:0006312 mitotic
recombination [PMID:17320816]"
/experiment="EXISTENCE:mutant phenotype:GO:0006338
chromatin remodeling [PMID:12887900]"
/experiment="EXISTENCE:mutant phenotype:GO:0006974 DNA
damage response [PMID:17320816]"
/experiment="EXISTENCE:physical interaction:GO:0031011
Ino80 complex [PMID:24034245|PMID:10952318]"
/note="Nuclear actin-related protein involved in chromatin
remodeling; component of chromatin-remodeling enzyme
complexes; has mRNA binding activity"
/codon_start=1
/product="Arp8p"
/protein_id="NP_014784.1"
/db_xref="GeneID:854309"
/db_xref="SGD:S000005667"
/translation="MSQEEAESSIIYEEPIDIPLEDDDDEDELEEENSVPLSSQADQE
NAENESDDSVDNVVGSETPRSVTGLSVDPRDVADEEDEDEEGEDEDEDEDDNDVDNED
ENDNDNANENENELGSSRDKRAPPAVQTSKRYKKYPKLDPAKAPPGKKVPLHLLEKRR
LGRIKAAEEFAKTLKKIGIEKVETTTLPATGLFQPLMLINQKNYSSDYLKKDDQIFAL
RDRKFLRNNNTSQISSTNTPDVIDLKSLPHSEASAAPLNDEIDLNDPTATIVIHPGSN
SIKIGFPKDDHPVVVPNCVAVPKKWLDLENSEHVENVCLQREQSEEFNNIKSEMEKNF
RERMRYYKRKVPGNAHEQVVSFNENSKPEIISEKNDPSPIEWIFDDSKLYYGSDALRC
VDEKFVIRKPFRGGSFNVKSPYYKSLAELISDVTKLLEHALNSETLNVKPTKFNQYKV
VLVIPDIFKKSHVETFIRVLLTELQFQAVAIIQESLATCYGAGISTSTCVVNIGAAET
RIACVDEGTVLEHSAITLDYGGDDITRLFALFLLQSDFPLQDWKIDSKHGWLLAERLK
KNFTTFQDADVAVQLYNFMNRSPNQPTEKYEFKLFDEVMLAPLALFFPQIFKLIRTSS
HKNSSLEFQLPESRDLFTNELNDWNSLSQFESKEGNLYCDLNDDLKILNRILDAHNII
DQLQDKPENYGNTLKENFAPLEKAIVQSIANASITADVTRMNSFYSNILIVGGSSKIP
ALDFILTDRINIWRPSLLSSASFPQFYKKLTKEIKDLEGHYVNAPDKTEDENKQILQA
QIKEKIVEELEEQHQNIEHQNGNEHIFPVSIIPPPRDMNPALIIWKGASVLAQIKLVE
ELFITNSDWDVHGSRILQYKCIFTY"
gene <593057..>594046
/gene="LSC1"
/locus_tag="YOR142W"
/db_xref="GeneID:854310"
mRNA <593057..>594046
/gene="LSC1"
/locus_tag="YOR142W"
/product="succinate--CoA ligase (GDP-forming) subunit
alpha"
/transcript_id="NM_001183561.3"
/db_xref="GeneID:854310"
CDS 593057..594046
/gene="LSC1"
/locus_tag="YOR142W"
/EC_number="6.2.1.5"
/experiment="EXISTENCE:direct assay:GO:0004775
succinate-CoA ligase (ADP-forming) activity
[PMID:9874242]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:24769239|PMID:9874242|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0006104
succinyl-CoA metabolic process [PMID:9874242]"
/experiment="EXISTENCE:direct assay:GO:0042645
mitochondrial nucleoid [PMID:15692048]"
/experiment="EXISTENCE:mutant phenotype:GO:0004775
succinate-CoA ligase (ADP-forming) activity
[PMID:9874242]"
/note="Alpha subunit of succinyl-CoA ligase; succinyl-CoA
ligase is a mitochondrial enzyme of the TCA cycle that
catalyzes the nucleotide-dependent conversion of
succinyl-CoA to succinate; phosphorylated"
/codon_start=1
/product="succinate--CoA ligase (GDP-forming) subunit
alpha"
/protein_id="NP_014785.3"
/db_xref="GeneID:854310"
/db_xref="SGD:S000005668"
/translation="MLRSTVSKASLKICRHFHRESIPYDKTIKNLLLPKDTKVIFQGF
TGKQGTFHASISQEYGTNVVGGTNPKKAGQTHLGQPVFASVKDAIKETGATASAIFVP
PPIAAAAIKESIEAEIPLAVCITEGIPQHDMLYIAEMLQTQDKTRLVGPNCPGIINPA
TKVRIGIQPPKIFQAGKIGIISRSGTLTYEAVQQTTKTDLGQSLVIGMGGDAFPGTDF
IDALKLFLEDETTEGIIMLGEIGGKAEIEAAQFLKEYNFSRSKPMPVASFIAGTVAGQ
MKGVRMGHSGAIVEGSGTDAESKKQALRDVGVAVVESPGYLGQALLDQFAKFK"
gene complement(594354..594425)
/gene="SUF5"
/locus_tag="YNCO0020C"
/db_xref="GeneID:854311"
tRNA complement(594354..594425)
/gene="SUF5"
/locus_tag="YNCO0020C"
/product="tRNA-Gly"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE
analysis; can mutate to suppress +1 frameshift mutations
in glycine codons"
/db_xref="GeneID:854311"
/db_xref="SGD:S000006573"
repeat_region 594512..594819
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007169"
mobile_element 594820..600733
/note="YORWTy1-2; Ty1 element, LTR retrotransposon of the
Copia (Pseudoviridae) group; contains co-transcribed genes
TYA Gag and TYB Pol, encoding proteins involved in
structure and function of virus-like particles, flanked by
two direct repeats"
/mobile_element_type="retrotransposon:YORWTy1-2"
/db_xref="SGD:S000007182"
repeat_region 594820..595151
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007170"
gene <595112..>600380
/locus_tag="YOR142W-B"
/db_xref="GeneID:854313"
mRNA <595112..>600380
/locus_tag="YOR142W-B"
/product="gag-pol fusion protein"
/transcript_id="NM_001184386.2"
/db_xref="GeneID:854313"
CDS join(595112..596416,596418..600380)
/locus_tag="YOR142W-B"
/EC_number="2.7.7.7"
/EC_number="2.7.7.49"
/EC_number="3.1.26.4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9448009]"
/ribosomal_slippage
/note="Retrotransposon TYA Gag and TYB Pol genes;
transcribed/translated as one unit; polyprotein is
processed to make a nucleocapsid-like protein (Gag),
reverse transcriptase (RT), protease (PR), and integrase
(IN); similar to retroviral genes"
/codon_start=1
/product="gag-pol fusion protein"
/protein_id="NP_058183.1"
/db_xref="GeneID:854313"
/db_xref="SGD:S000007352"
/translation="MESQQLSNYPHISHGSACASVTSKEVHTNQDPLDVSASKIQEYD
KASTKANSQQTTTPASSAVPENPHHASPQPASVPPPQNGPYPQQCMMTQNQANPSGWS
FYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVRPITDDELT
FLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQ
YNGSTPADAFETKVTNIIDRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNM
TVAELFLDIHAIYEEQQGSRNSKPNYRRNPSDEKNDSRSYTNTTKPKVIARNPQKTNN
SKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLGQKLTESTVNHTNHS
DDELPGHLLLDSGASRTLIRSAHHIHSASSNPDINVVDAQKRNIPINAIGDLQFHFQD
NTKTSIKVLHTPNIAYDLLSLNELAAVDITACFTKNVLERSDGTVLAPIVKYGDFYWV
SKKYLLPSNISVPTINNVHTSESTRKYPYPFIHRMLAHANAQTIRYSLKNNTITYFNE
SDVDWSSAIDYQCPDCLIGKSTKHRHIKGSRLKYQNSYEPFQYLHTDIFGPVHNLPKS
APSYFISFTDETTKFRWVYPLHDRREDSILDVFTTILAFIKNQFQASVLVIQMDRGSE
YTNRTLHKFLEKNGITPCYTTTADSRAHGVAERLNRTLLDDCRTQLQCSGLPNHLWFS
AIEFSTIVRNSLASPKSKKSARQHAGLAGLDISTLLPFGQPVIVNDHNPNSKIHPRGI
PGYALHPSRNSYGYIIYLPSLKKTVDTTNYVILQGKESRLDQFNYDALTFDEDLNRLT
ASYHSFIASNEIQESNDLNIESDHDFQSDIELHPEQPRNVLSKAVSPTDSTPPSTHTE
DSKRVSKTNIRAPREVDPNISESNILPSKKRSSTPQISNIESTGSGGMHKLNVPLLAP
MSQSNTHESSHASKSKDFRHSDSYSENETNHTNVPISSTGGTNNKTVPQISDQETEKR
IIHRSPSIDASPPENNSSHNIVPIKTPTTVSEQNTEESIIADLPLPDLPPESPTEFPD
PFKELPPINSRQTNSSLGGIGDSNAYTTINSKKRSLEDNETEIKVSRDTWNTKNMRSL
EPPRSKKRIHLIAAVKAVKSIKPIRTTLRYDEAITYNKDIKEKEKYIEAYHKEVNQLL
KMKTWDTDKYYDRKEIDPKRVINSMFIFNRKRDGTHKARFVARGDIQHPDTYDSGMQS
NTVHHYALMTSLSLALDNNYYITQLDISSAYLYADIKEELYIRPPPHLGMNDKLIRLK
KSLYGLKQSGANWYETIKSYLIKQCGMEEVRGWSCVFKNSQVTICLFVDDMILFSKDL
NSNKRIIAKLKMQYDTKIINLGESDDEIQYDILGLEIKYQRGKYMKLGMENSLTEKIP
KLNVPLNPNGRKLGAPGQPGLYINQQELELEEDDYKMKVHEMQKLIGLASYVGYKFRF
DLLYYINTLAQHILFPSKQVLDMTYELIQFIWNTRDKQLIWHKSKPVKPTNKLVVISD
ASYGNQPYYKSQIGNIYLLNGKVIGGKSTKASLTCTSTTEAEIHAISESVPLLNNLSY
LIQELDKKPITKGLLTDSKSTISIIISNNEEKFRNRFFGTKAMRLRDEVSGNHLHVCY
IETKKNIADVMTKPLPIKTFKLLTNKWIH"
gene <595112..>596434
/locus_tag="YOR142W-A"
/db_xref="GeneID:854312"
mRNA <595112..>596434
/locus_tag="YOR142W-A"
/product="gag protein"
/transcript_id="NM_001184385.3"
/db_xref="GeneID:854312"
CDS 595112..596434
/locus_tag="YOR142W-A"
/note="Retrotransposon TYA Gag gene co-transcribed with
TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is
a nucleocapsid protein that is the structural constituent
of virus-like particles (VLPs); similar to retroviral Gag"
/codon_start=1
/product="gag protein"
/protein_id="NP_058184.3"
/db_xref="GeneID:854312"
/db_xref="SGD:S000007351"
/translation="MESQQLSNYPHISHGSACASVTSKEVHTNQDPLDVSASKIQEYD
KASTKANSQQTTTPASSAVPENPHHASPQPASVPPPQNGPYPQQCMMTQNQANPSGWS
FYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVRPITDDELT
FLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQ
YNGSTPADAFETKVTNIIDRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNM
TVAELFLDIHAIYEEQQGSRNSKPNYRRNPSDEKNDSRSYTNTTKPKVIARNPQKTNN
SKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLRPETY"
repeat_region 600402..600733
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007171"
rep_origin 600886..600960
/note="ARS1502; Autonomously Replicating Sequence"
/db_xref="SGD:S000007643"
gene complement(<601383..>602342)
/gene="THI80"
/locus_tag="YOR143C"
/db_xref="GeneID:854314"
mRNA complement(<601383..>602342)
/gene="THI80"
/locus_tag="YOR143C"
/product="thiamine diphosphokinase"
/transcript_id="NM_001183562.1"
/db_xref="GeneID:854314"
CDS complement(601383..602342)
/gene="THI80"
/locus_tag="YOR143C"
/EC_number="2.7.6.2"
/experiment="EXISTENCE:direct assay:GO:0004788 thiamine
diphosphokinase activity [PMID:18652651|PMID:8394343]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0004788
thiamine diphosphokinase activity [PMID:8394343]"
/experiment="EXISTENCE:mutant phenotype:GO:0009229
thiamine diphosphate biosynthetic process [PMID:8394343]"
/note="Thiamine pyrophosphokinase; phosphorylates thiamine
to produce the coenzyme thiamine pyrophosphate (thiamine
diphosphate)"
/codon_start=1
/product="thiamine diphosphokinase"
/protein_id="NP_014786.1"
/db_xref="GeneID:854314"
/db_xref="SGD:S000005669"
/translation="MSEECIENPERIKIGTDLINIRNKMNLKELIHPNEDENSTLLIL
NQKIDIPRPLFYKIWKLHDLKVCADGAANRLYDYLDDDETLRIKYLPNYIIGDLDSLS
EKVYKYYRKNKVTIIKQTTQYSTDFTKCVNLISLHFNSPEFRSLISNKDNLQSNHGIE
LEKGIHTLYNTMTESLVFSKVTPISLLALGGIGGRFDQTVHSITQLYTLSENASYFKL
CYMTPTDLIFLIKKNGTLIEYDPQFRNTCIGNCGLLPIGEATLVKETRGLKWDVKNWP
TSVVTGRVSSSNRFVGDNCCFIDTKDDIILNVEIFVDKLIDFL"
gene complement(<602717..>605092)
/gene="ELG1"
/locus_tag="YOR144C"
/gene_synonym="RTT110"
/db_xref="GeneID:854315"
mRNA complement(<602717..>605092)
/gene="ELG1"
/locus_tag="YOR144C"
/gene_synonym="RTT110"
/product="Elg1p"
/transcript_id="NM_001183563.3"
/db_xref="GeneID:854315"
CDS complement(602717..605092)
/gene="ELG1"
/locus_tag="YOR144C"
/gene_synonym="RTT110"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:18482875]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:genetic interaction:GO:0007064
mitotic sister chromatid cohesion [PMID:19430531]"
/experiment="EXISTENCE:mutant phenotype:GO:0000724
double-strand break repair via homologous recombination
[PMID:17170004]"
/experiment="EXISTENCE:mutant phenotype:GO:0006260 DNA
replication [PMID:23499004]"
/experiment="EXISTENCE:mutant phenotype:GO:0006261
DNA-templated DNA replication [PMID:12912927]"
/experiment="EXISTENCE:mutant phenotype:GO:0007064 mitotic
sister chromatid cohesion [PMID:19430531]"
/experiment="EXISTENCE:mutant phenotype:GO:0010526
transposable element silencing [PMID:11779788]"
/experiment="EXISTENCE:mutant phenotype:GO:0031507
heterochromatin formation [PMID:29440488]"
/experiment="EXISTENCE:physical interaction:GO:0031391
Elg1 RFC-like complex [PMID:12909721]"
/note="Subunit of an alternative replication factor C
complex; important for DNA replication and genome
integrity; suppresses spontaneous DNA damage; involved in
homologous recombination-mediated repair and telomere
homeostasis; required for PCNA (Pol30p) unloading during
DNA replication"
/codon_start=1
/product="Elg1p"
/protein_id="NP_014787.3"
/db_xref="GeneID:854315"
/db_xref="SGD:S000005670"
/translation="MKRHVSLSDILTGNKRKVRRQDALQITIDDENDTESGTFDARTA
KHDDSSVIFLNHSVVKPIEAVSTNHKSAKEFLMTKRTKEKCDDDDDDLIVISDKSPKS
ETNCSKIALSQEHEDDISIISTSRIKSSLLNERASKIKNFLKHETTDTFKRLNSISKL
NEIEPPLPLHQSIFPVGDKELSDRSVDIPLPFRTIPPLNHNFLPSDYESLKDKNSASC
IPVRYQAPVLLGTNIKRNTTLTWPQLFKPVTLKQVLIEPKLKLRIKNWIETSFHTLEK
PTLRNRLLNRINPNKQQGSGDELANFIVPDLEEDENLRPDFYRNGEANSSLSEFVPLM
ILHGNSIGKKTLIQTIMREIAGDDNSYQIYEVNSNMNRSKKDLLDILLDFTTTHYVKD
SSKRKSDYGLVLFNDVDVLFKEHDRGYWAMISKLCEFSRRPLVLTCKDLSLVPSELIA
LASEQNSLFHTKKISTSTVYAFLTKYLKSLEIEVCDDWLRDVVKQNNADIRKCLMHLQ
FWCVDTEADLISSKNRLPVLTSTLGSSVKDISQLTDLLSINDVIGQATLNRSMVRQEI
DSTTMTPEKVNTFQDQNLDDEMKLKFDYVIDYKLHLNDPNRQPLLPFELNIYQHIQEQ
LEARYSYVREANHRLDNEYLVNRFKKMTESTLNFLASRIPKYDHLQSARRTRNSKKIS
DILNQFKGIYNDETLNENAEIDLLSATTQQIKAEINPFVFEIAKSDANVKNENKQIFE
LHSENVSERRYKDLVYQLSQEGVLKNVWFNADPSIVVRKWEHLHSGFSKNK"
gene complement(<605347..>606171)
/gene="PNO1"
/locus_tag="YOR145C"
/gene_synonym="DIM2; RRP20"
/db_xref="GeneID:854317"
mRNA complement(<605347..>606171)
/gene="PNO1"
/locus_tag="YOR145C"
/gene_synonym="DIM2; RRP20"
/product="Pno1p"
/transcript_id="NM_001183564.1"
/db_xref="GeneID:854317"
CDS complement(605347..606171)
/gene="PNO1"
/locus_tag="YOR145C"
/gene_synonym="DIM2; RRP20"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12502737]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:10923024]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/experiment="EXISTENCE:direct assay:GO:0042134 rRNA
primary transcript binding [PMID:18755838]"
/experiment="EXISTENCE:direct assay:GO:0051082 unfolded
protein binding [PMID:12502737]"
/experiment="EXISTENCE:mutant phenotype:GO:0000056
ribosomal small subunit export from nucleus
[PMID:18755838]"
/experiment="EXISTENCE:mutant phenotype:GO:0000447
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from
5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12736301]"
/experiment="EXISTENCE:mutant phenotype:GO:0000472
endonucleolytic cleavage to generate mature 5'-end of
SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:12736301]"
/experiment="EXISTENCE:mutant phenotype:GO:0042255
ribosome assembly [PMID:18755838]"
/experiment="EXISTENCE:mutant phenotype:GO:0043248
proteasome assembly [PMID:12502737]"
/experiment="EXISTENCE:physical interaction:GO:0043248
proteasome assembly [PMID:12502737]"
/note="Essential nucleolar protein required for pre-18S
rRNA processing; interacts with Dim1p, an 18S rRNA
dimethyltransferase, and also with Nob1p, which is
involved in proteasome biogenesis; contains a KH domain"
/codon_start=1
/product="Pno1p"
/protein_id="NP_014788.1"
/db_xref="GeneID:854317"
/db_xref="SGD:S000005671"
/translation="MVAPTALKKATVTPVSGQDGGSSRIIGINNTESIDEDDDDDVLL
DDSDNNTAKEEVEGEEGSRKTHESKTVVVDDQGKPRFTSASKTQGNKIKFESRKIMVP
PHRMTPLRNSWTKIYPPLVEHLKLQVRMNLKTKSVELRTNPKFTTDPGALQKGADFIK
AFTLGFDLDDSIALLRLDDLYIETFEVKDVKTLTGDHLSRAIGRIAGKDGKTKFAIEN
ATRTRIVLADSKIHILGGFTHIRMARESVVSLILGSPPGKVYGNLRTVASRLKERY"
gene <606607..>608475
/gene="MDM32"
/locus_tag="YOR147W"
/db_xref="GeneID:854318"
mRNA <606607..>608475
/gene="MDM32"
/locus_tag="YOR147W"
/product="Mdm32p"
/transcript_id="NM_001183566.3"
/db_xref="GeneID:854318"
CDS 606607..608475
/gene="MDM32"
/locus_tag="YOR147W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:15631992]"
/experiment="EXISTENCE:mutant phenotype:GO:0000001
mitochondrion inheritance [PMID:15631992]"
/experiment="EXISTENCE:mutant phenotype:GO:0006873
intracellular monoatomic ion homeostasis [PMID:16020778]"
/experiment="EXISTENCE:mutant phenotype:GO:0007005
mitochondrion organization [PMID:11907266|PMID:16020778]"
/note="Mitochondrial inner membrane protein with
similarity to Mdm31p; required for normal mitochondrial
morphology and inheritance; interacts genetically with
MMM1, MDM10, MDM12, and MDM34; variation between SK1 and
S288C at residues 182 and 262 impacts invasive growth and
mitochondrial network structure"
/codon_start=1
/product="Mdm32p"
/protein_id="NP_014790.4"
/db_xref="GeneID:854318"
/db_xref="SGD:S000005673"
/translation="MLITRLRVPTIKRPLLPITSHLVRHCIRTYVATNHGNVRPFITP
YKSSLPVRCLIAQRHIRTFPSNDKFTTKASNIETILLRKNNEREFKQSLLADAKNFQE
RFKINLKWILIKNNRPFSLNEISIIASWLILSQILWLILSTTTFISFYLFVINSVFSQ
EYIHEKKIYERLLKWLLKDHKCSNQDLEITFSPEDKASMLVLSPDWESNSILIKRLNV
RDEILDLDLKFHHINLNVSLKNWLLGRGLITNVSIYGIRGCLNLSNFINLVNSFQGDQ
KTENFLKTLNNVEITDSEILLKQSLSAQETPSLKFSIYNLSLPRLRLNHFISDILSAK
TFSGSINNSLFNLFKRQQKLTAVIENNNKNRMASSKFDFTDNNQENYRTVTHQDDPNY
VTTLRLNFININDLKFNGDGKFNWLKDGQVEILADIMLTNSTSHLSSESKYAVVDLKV
TCRDLKTTFPQEPPVLSTGDSIVSLDELKPIITFINSYEGMANPILKDFSENERLTNS
IIWNSPNVSINRQRKSYPLTTKVTSNSTKEIIKFHNQPNTNANEIVLRCKMVKNLSDL
QLININQILDQITMELYVDLTKIVEDWEFKNKNDWMKQWGTTFASQLLLFGFGAMV"
gene complement(<608640..>609197)
/gene="SPP2"
/locus_tag="YOR148C"
/db_xref="GeneID:854319"
mRNA complement(<608640..>609197)
/gene="SPP2"
/locus_tag="YOR148C"
/product="spliceosome ATPase-activating subunit SPP2"
/transcript_id="NM_001183567.1"
/db_xref="GeneID:854319"
CDS complement(608640..609197)
/gene="SPP2"
/locus_tag="YOR148C"
/experiment="EXISTENCE:direct assay:GO:0001671 ATPase
activator activity [PMID:25561498]"
/experiment="EXISTENCE:direct assay:GO:0005681
spliceosomal complex [PMID:7493316]"
/experiment="EXISTENCE:mutant phenotype:GO:0000398 mRNA
splicing, via spliceosome [PMID:7493316]"
/note="Essential protein that promotes the first step of
splicing; required for the final stages of spliceosome
maturation and activation; interacts with Prp2p, which may
release Spp2p from the spliceosome following the first
cleavage reaction; stimulates Prp2p ATPase activity"
/codon_start=1
/product="spliceosome ATPase-activating subunit SPP2"
/protein_id="NP_014791.1"
/db_xref="GeneID:854319"
/db_xref="SGD:S000005674"
/translation="MSKFSLKLGSKTLKKNISKKTKKKNSLQKANLFDWDDAETASLS
HKPQSKIKIQSIDKFDLDEESSSKKKLVIKLSENADTKKNDAPLVEYVTEKEYNEVPV
EEFGDALLRGMGWESDSEQDSKGDKTQSRNKDVSNVSQIHPDGLGIGAKLNKAINVEE
ASFMPVVKIDKITGTKVDDDKKNKS"
gene complement(<609838..>611388)
/gene="SMP3"
/locus_tag="YOR149C"
/gene_synonym="LAS2; SAP2"
/db_xref="GeneID:854320"
mRNA complement(<609838..>611388)
/gene="SMP3"
/locus_tag="YOR149C"
/gene_synonym="LAS2; SAP2"
/product="glycosylphosphatidylinositol-alpha 1,2
mannosyltransferase"
/transcript_id="NM_001183568.4"
/db_xref="GeneID:854320"
CDS complement(609838..611388)
/gene="SMP3"
/locus_tag="YOR149C"
/gene_synonym="LAS2; SAP2"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0000026
alpha-1,2-mannosyltransferase activity [PMID:11356840]"
/experiment="EXISTENCE:mutant phenotype:GO:0006276 plasmid
maintenance [PMID:2005867]"
/experiment="EXISTENCE:mutant phenotype:GO:0006506 GPI
anchor biosynthetic process [PMID:11356840]"
/note="Alpha 1,2-mannosyltransferase; involved in glycosyl
phosphatidyl inositol (GPI) biosynthesis; required for
addition of the fourth, side branching mannose to the GPI
core structure"
/codon_start=1
/product="glycosylphosphatidylinositol-alpha 1,2
mannosyltransferase"
/protein_id="NP_014792.4"
/db_xref="GeneID:854320"
/db_xref="SGD:S000005675"
/translation="MMRYQWWLYLVYAIGLMLCLGPSYIHPDEHFQCIEILAMQFMKV
KGTIPWEFKSKFAARSYGPLLLVYGPLFTILESFPEIQDNPALILYSMRLQNYVMYLL
CYHFLIPKLIRDERKAVQFIKKSLLLTSYVTWTYQTHTFSNSIETLALISTLTVMEDM
VNEKNIQRSNFKNSVILGLIFSFGVFNRVTFPAFIFLPCLILFWKFYRVHWKSFSLLL
LSFSFSSCLFVLIDTNIYNNGKGFVITPLNNLKYNLNVQNLQVHGLHPRYTHLLVNLP
QIVGPVLLLAIFSGYKLDKLSTYAIISGLLFLSFFQHQELRFLVPLVPLLVTNLNWTP
LSSTLVNKKIFKGTWLLFNIIMAFIMGISHQAGIIQFLGDYFHFRTEQMGVHIWWKTY
SPPTWMYMSNNLTVSSLINTQDGIESIDEVAFSVGNHHVIDLKGCDLPLLTETIRRLR
LNGSITPLTLVTPNSMTSELKKLKRDGTINLIPKRNYLFHLDLDHLDFNDFTTFKPGL
TVYSIELL"
gene <611999..>612490
/gene="MRPL23"
/locus_tag="YOR150W"
/gene_synonym="uL13m"
/db_xref="GeneID:854321"
mRNA <611999..>612490
/gene="MRPL23"
/locus_tag="YOR150W"
/gene_synonym="uL13m"
/product="mitochondrial 54S ribosomal protein uL13m
MRPL23"
/transcript_id="NM_001183569.1"
/db_xref="GeneID:854321"
CDS 611999..612490
/gene="MRPL23"
/locus_tag="YOR150W"
/gene_synonym="uL13m"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:9151978|PMID:12392552]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:12392552|PMID:9151978]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005762
mitochondrial large ribosomal subunit
[PMID:9151978|PMID:12392552]"
/note="Mitochondrial ribosomal protein of the large
subunit; localizes to vacuole in response to H2O2"
/codon_start=1
/product="mitochondrial 54S ribosomal protein uL13m
MRPL23"
/protein_id="NP_014793.1"
/db_xref="GeneID:854321"
/db_xref="SGD:S000005676"
/translation="MSQKIGHSGLAFARLWHHVDVARDKRTLGRLASAIAITLIGRHK
PVYHPSQDCGDYVVVTNCQKIRVTGKKFEQKTYWSHSGRPGQLKLQTMNKVVADKGFG
EILKKAVSGMLPKNKLRKQRLDRLKVFDGSENPYKQNITAFAHEQSSIPEPLKESIFN
QLK"
gene complement(<612997..>616671)
/gene="RPB2"
/locus_tag="YOR151C"
/gene_synonym="RPB150; RPO22; SIT2; SOH2"
/db_xref="GeneID:854322"
mRNA complement(<612997..>616671)
/gene="RPB2"
/locus_tag="YOR151C"
/gene_synonym="RPB150; RPO22; SIT2; SOH2"
/product="DNA-directed RNA polymerase II core subunit
RPB2"
/transcript_id="NM_001183570.3"
/db_xref="GeneID:854322"
CDS complement(612997..616671)
/gene="RPB2"
/locus_tag="YOR151C"
/gene_synonym="RPB150; RPO22; SIT2; SOH2"
/EC_number="2.7.7.6"
/experiment="EXISTENCE:direct assay:GO:0003677 DNA binding
[PMID:26945063]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0003899
DNA-directed RNA polymerase activity [PMID:8288647]"
/experiment="EXISTENCE:direct assay:GO:0003968
RNA-directed RNA polymerase activity [PMID:18004386]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005665 RNA
polymerase II, core complex
[PMID:2186966|PMID:2183013|PMID:1331084]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405]"
/experiment="EXISTENCE:mutant phenotype:GO:0006366
transcription by RNA polymerase II [PMID:2406558]"
/note="RNA polymerase II second largest subunit B150; part
of central core; similar to bacterial beta subunit"
/codon_start=1
/product="DNA-directed RNA polymerase II core subunit
RPB2"
/protein_id="NP_014794.3"
/db_xref="GeneID:854322"
/db_xref="SGD:S000005677"
/translation="MSDLANSEKYYDEDPYGFEDESAPITAEDSWAVISAFFREKGLV
SQQLDSFNQFVDYTLQDIICEDSTLILEQLAQHTTESDNISRKYEISFGKIYVTKPMV
NESDGVTHALYPQEARLRNLTYSSGLFVDVKKRTYEAIDVPGRELKYELIAEESEDDS
ESGKVFIGRLPIMLRSKNCYLSEATESDLYKLKECPFDMGGYFIINGSEKVLIAQERS
AGNIVQVFKKAAPSPISHVAEIRSALEKGSRFISTLQVKLYGREGSSARTIKATLPYI
KQDIPIVIIFRALGIIPDGEILEHICYDVNDWQMLEMLKPCVEDGFVIQDRETALDFI
GRRGTALGIKKEKRIQYAKDILQKEFLPHITQLEGFESRKAFFLGYMINRLLLCALDR
KDQDDRDHFGKKRLDLAGPLLAQLFKTLFKKLTKDIFRYMQRTVEEAHDFNMKLAINA
KTITSGLKYALATGNWGEQKKAMSSRAGVSQVLNRYTYSSTLSHLRRTNTPIGRDGKL
AKPRQLHNTHWGLVCPAETPEGQACGLVKNLSLMSCISVGTDPMPIITFLSEWGMEPL
EDYVPHQSPDATRVFVNGVWHGVHRNPARLMETLRTLRRKGDINPEVSMIRDIREKEL
KIFTDAGRVYRPLFIVEDDESLGHKELKVRKGHIAKLMATEYQDIEGGFEDVEEYTWS
SLLNEGLVEYIDAEEEESILIAMQPEDLEPAEANEENDLDVDPAKRIRVSHHATTFTH
CEIHPSMILGVAASIIPFPDHNQSPRNTYQSAMGKQAMGVFLTNYNVRMDTMANILYY
PQKPLGTTRAMEYLKFRELPAGQNAIVAIACYSGYNQEDSMIMNQSSIDRGLFRSLFF
RSYMDQEKKYGMSITETFEKPQRTNTLRMKHGTYDKLDDDGLIAPGVRVSGEDVIIGK
TTPISPDEEELGQRTAYHSKRDASTPLRSTENGIVDQVLVTTNQDGLKFVKVRVRTTK
IPQIGDKFASRHGQKGTIGITYRREDMPFTAEGIVPDLIINPHAIPSRMTVAHLIECL
LSKVAALSGNEGDASPFTDITVEGISKLLREHGYQSRGFEVMYNGHTGKKLMAQIFFG
PTYYQRLRHMVDDKIHARARGPMQVLTRQPVEGRSRDGGLRFGEMERDCMIAHGAASF
LKERLMEASDAFRVHICGICGLMTVIAKLNHNQFECKGCDNKIDIYQIHIPYAAKLLF
QELMAMNITPRLYTDRSRDF"
rep_origin 616670..617517
/note="ARS1518; Putative replication origin; identified in
multiple array studies, not yet confirmed by plasmid-based
assay"
/db_xref="SGD:S000130136"
gene complement(<617518..>618288)
/gene="ATG40"
/locus_tag="YOR152C"
/db_xref="GeneID:854323"
mRNA complement(<617518..>618288)
/gene="ATG40"
/locus_tag="YOR152C"
/product="Atg40p"
/transcript_id="NM_001183571.1"
/db_xref="GeneID:854323"
CDS complement(617518..618288)
/gene="ATG40"
/locus_tag="YOR152C"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:11935221]"
/experiment="EXISTENCE:direct assay:GO:0032541 cortical
endoplasmic reticulum [PMID:26040717]"
/experiment="EXISTENCE:genetic interaction:GO:0006995
cellular response to nitrogen starvation [PMID:26040717]"
/experiment="EXISTENCE:genetic interaction:GO:0030674
protein-macromolecule adaptor activity [PMID:26040717]"
/experiment="EXISTENCE:genetic interaction:GO:0061709
reticulophagy [PMID:26040717]"
/experiment="EXISTENCE:mutant phenotype:GO:0006995
cellular response to nitrogen starvation [PMID:26040717]"
/experiment="EXISTENCE:mutant phenotype:GO:0030674
protein-macromolecule adaptor activity [PMID:26040717]"
/experiment="EXISTENCE:mutant phenotype:GO:0061709
reticulophagy [PMID:26040717]"
/note="Autophagy receptor with a role in endoplasmic
reticulum degradation; involved specifically in autophagy
of cortical and cytoplasmic ER in response to nitrogen
starvation or rapamycin treatment; acts with COPII subunit
Sfb3p; localizes to the cortical and cytoplasmic ER;
similar to human FAM134B, which is also involved in ER
autophagy and is associated with sensory neuropathy"
/codon_start=1
/product="Atg40p"
/protein_id="NP_014795.1"
/db_xref="GeneID:854323"
/db_xref="SGD:S000005678"
/translation="MFNLILWPLFLLTSVAIPLQLTLEVVYLTSSVDFSKASAAKTAT
SLGQSPVVITIYKSLLKYWSLYEFIHFIYLYTPIDAFLNFLPFTSLLMSFGSICLTRE
LVYDFIAFMESQNKLTGFLNKITEPNFNSYLLFSSIYNIWFADDTNDKFLFGKLTQIL
ISVTKRYEFPRTFYLAKVSDFLQNLILTRLRPFVTEQPQGDKNRYQNGDRESTKNGAA
YQKSSQQSSSFEQNFTSTEFPNDYDFMEDILDETTELD"
gene <619840..>624375
/gene="PDR5"
/locus_tag="YOR153W"
/gene_synonym="LEM1; STS1; YDR1"
/db_xref="GeneID:854324"
mRNA <619840..>624375
/gene="PDR5"
/locus_tag="YOR153W"
/gene_synonym="LEM1; STS1; YDR1"
/product="ATP-binding cassette multidrug transporter PDR5"
/transcript_id="NM_001183572.3"
/db_xref="GeneID:854324"
CDS 619840..624375
/gene="PDR5"
/locus_tag="YOR153W"
/gene_synonym="LEM1; STS1; YDR1"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:12469340|PMID:8810273]"
/experiment="EXISTENCE:direct assay:GO:0008559 ABC-type
xenobiotic transporter activity [PMID:21207971]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0030003
intracellular monoatomic cation homeostasis
[PMID:8985171]"
/experiment="EXISTENCE:mutant phenotype:GO:0008559
ABC-type xenobiotic transporter activity [PMID:15707993]"
/experiment="EXISTENCE:mutant phenotype:GO:0009410
response to xenobiotic stimulus [PMID:17881724]"
/experiment="EXISTENCE:mutant phenotype:GO:0030003
intracellular monoatomic cation homeostasis
[PMID:8985171]"
/experiment="EXISTENCE:mutant phenotype:GO:1990961
xenobiotic detoxification by transmembrane export across
the plasma membrane [PMID:9450972]"
/note="Plasma membrane ATP-binding cassette (ABC)
transporter; multidrug transporter actively regulated by
Pdr1p; also involved in steroid transport, cation
resistance, and cellular detoxification during exponential
growth; PDR5 has a paralog, PDR15, that arose from the
whole genome duplication"
/codon_start=1
/product="ATP-binding cassette multidrug transporter PDR5"
/protein_id="NP_014796.3"
/db_xref="GeneID:854324"
/db_xref="SGD:S000005679"
/translation="MPEAKLNNNVNDVTSYSSASSSTENAADLHNYNGFDEHTEARIQ
KLARTLTAQSMQNSTQSAPNKSDAQSIFSSGVEGVNPIFSDPEAPGYDPKLDPNSENF
SSAAWVKNMAHLSAADPDFYKPYSLGCAWKNLSASGASADVAYQSTVVNIPYKILKSG
LRKFQRSKETNTFQILKPMDGCLNPGELLVVLGRPGSGCTTLLKSISSNTHGFDLGAD
TKISYSGYSGDDIKKHFRGEVVYNAEADVHLPHLTVFETLVTVARLKTPQNRIKGVDR
ESYANHLAEVAMATYGLSHTRNTKVGNDIVRGVSGGERKRVSIAEVSICGSKFQCWDN
ATRGLDSATALEFIRALKTQADISNTSATVAIYQCSQDAYDLFNKVCVLDDGYQIYYG
PADKAKKYFEDMGYVCPSRQTTADFLTSVTSPSERTLNKDMLKKGIHIPQTPKEMNDY
WVKSPNYKELMKEVDQRLLNDDEASREAIKEAHIAKQSKRARPSSPYTVSYMMQVKYL
LIRNMWRLRNNIGFTLFMILGNCSMALILGSMFFKIMKKGDTSTFYFRGSAMFFAILF
NAFSSLLEIFSLYEARPITEKHRTYSLYHPSADAFASVLSEIPSKLIIAVCFNIIFYF
LVDFRRNGGVFFFYLLINIVAVFSMSHLFRCVGSLTKTLSEAMVPASMLLLALSMYTG
FAIPKKKILRWSKWIWYINPLAYLFESLLINEFHGIKFPCAEYVPRGPAYANISSTES
VCTVVGAVPGQDYVLGDDFIRGTYQYYHKDKWRGFGIGMAYVVFFFFVYLFLCEYNEG
AKQKGEILVFPRSIVKRMKKRGVLTEKNANDPENVGERSDLSSDRKMLQESSEEESDT
YGEIGLSKSEAIFHWRNLCYEVQIKAETRRILNNVDGWVKPGTLTALMGASGAGKTTL
LDCLAERVTMGVITGDILVNGIPRDKSFPRSIGYCQQQDLHLKTATVRESLRFSAYLR
QPAEVSIEEKNRYVEEVIKILEMEKYADAVVGVAGEGLNVEQRKRLTIGVELTAKPKL
LVFLDEPTSGLDSQTAWSICQLMKKLANHGQAILCTIHQPSAILMQEFDRLLFMQRGG
KTVYFGDLGEGCKTMIDYFESHGAHKCPADANPAEWMLEVVGAAPGSHANQDYYEVWR
NSEEYRAVQSELDWMERELPKKGSITAAEDKHEFSQSIIYQTKLVSIRLFQQYWRSPD
YLWSKFILTIFNQLFIGFTFFKAGTSLQGLQNQMLAVFMFTVIFNPILQQYLPSFVQQ
RDLYEARERPSRTFSWISFIFAQIFVEVPWNILAGTIAYFIYYYPIGFYSNASAAGQL
HERGALFWLFSCAFYVYVGSMGLLVISFNQVAESAANLASLLFTMSLSFCGVMTTPSA
MPRFWIFMYRVSPLTYFIQALLAVGVANVDVKCADYELLEFTPPSGMTCGQYMEPYLQ
LAKTGYLTDENATDTCSFCQISTTNDYLANVNSFYSERWRNYGIFICYIAFNYIAGVF
FYWLARVPKKNGKLSKK"
gene <624729..>626492
/gene="SLP1"
/locus_tag="YOR154W"
/db_xref="GeneID:854325"
mRNA <624729..>626492
/gene="SLP1"
/locus_tag="YOR154W"
/product="Slp1p"
/transcript_id="NM_001183573.1"
/db_xref="GeneID:854325"
CDS 624729..626492
/gene="SLP1"
/locus_tag="YOR154W"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:23275891]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0034975
protein folding in endoplasmic reticulum [PMID:19325107]"
/note="Glycosylated integral ER membrane hypothetical
protein; forms an ER-membrane associated protein complex
with Emp65p; member of the SUN-like family of proteins;
genetic interactions suggest a role in folding of ER
membrane proteins; required for nuclear envelope
localization of Mps3p"
/codon_start=1
/product="Slp1p"
/protein_id="NP_014797.1"
/db_xref="GeneID:854325"
/db_xref="SGD:S000005680"
/translation="MANRLLIYGLILWVSIIGSFALDRNKTAQNAKIGLHDTTVITTG
STTNVQKEHSSPLSTGSLRTHDFRQASKVDIRQADIRENGERKEQDALTQPATPRNPG
DSSNSFLSFDEWKKVKSKEHSSGPERHLSRVREPVDPSCYKEKECIGEELEIDLGFLT
NKNEWSEREENQKGFNEEKDIEKVYKKKFNYASLDCAATIVKSNPEAIGATSTLIESK
DKYLLNPCSAPQQFIVIELCEDILVEEIEIANYEFFSSTFKRFRVSVSDRIPMVKNEW
TILGEFEARNSRELQKFQIHNPQIWASYLKIEILSHYEDEFYCPISLIKVYGKSMMDE
FKIDQLKAQEDKEQSIGTNNINNLNEQNIQDRCNNIETRLETPNTSNLSDLAGALSCT
SKLIPLKFDEFFKVLNASFCPSKQMISSSSSSAVPVIPEESIFKNIMKRLSQLETNSS
LTVSYIEEQSKLLSKSFEQLEMAHEAKFSHLVTIFNETMMSNLDLLNNFANQLKDQSL
RILEEQKLENDKFTNRHLLHLERLEKEVSFQRRIVYASFFAFVGLISYLLITRELYFE
DFEESKNGAIEKADIVQQAIR"
gene complement(<626628..>627980)
/gene="ISN1"
/locus_tag="YOR155C"
/db_xref="GeneID:854326"
mRNA complement(<626628..>627980)
/gene="ISN1"
/locus_tag="YOR155C"
/product="IMP 5'-nucleotidase"
/transcript_id="NM_001183574.3"
/db_xref="GeneID:854326"
CDS complement(626628..627980)
/gene="ISN1"
/locus_tag="YOR155C"
/EC_number="3.1.3.99"
/experiment="EXISTENCE:direct assay:GO:0008253
5'-nucleotidase activity [PMID:12735798]"
/experiment="EXISTENCE:genetic interaction:GO:0071590
nicotinamide riboside biosynthetic process
[PMID:19846558]"
/experiment="EXISTENCE:genetic interaction:GO:0071592
nicotinic acid riboside biosynthetic process
[PMID:19846558]"
/experiment="EXISTENCE:mutant phenotype:GO:0006190 inosine
salvage [PMID:12735798]"
/experiment="EXISTENCE:mutant phenotype:GO:0071590
nicotinamide riboside biosynthetic process
[PMID:19846558]"
/experiment="EXISTENCE:mutant phenotype:GO:0071592
nicotinic acid riboside biosynthetic process
[PMID:19846558]"
/note="Inosine 5'-monophosphate (IMP)-specific
5'-nucleotidase; catalyzes the breakdown of IMP to
inosine; responsible for production of nicotinamide
riboside and nicotinic acid riboside; tetrameric form
negatively regulated by inosine and ATP; expression
positively regulated by nicotinic acid and glucose
availability; does not show similarity to known
5'-nucleotidases from other organisms"
/codon_start=1
/product="IMP 5'-nucleotidase"
/protein_id="NP_014798.3"
/db_xref="GeneID:854326"
/db_xref="SGD:S000005681"
/translation="MSSRYRVEYHLKSHRKDEFIDWVKGLLASPFVLHAVSHEGDYND
DLATTQRVRSQYADIFKDIEGLIKDKIEFDSRNMSQDEIEDGASSQSLNILGQSRLNL
LVPSIGTFFTELPLEQAFLWEDSQRAISARRMVAPSFNDIRHILNTAQIFHFKKQENL
HNGKVLRLVTFDGDVTLYEDGGSLVYTNPVIPYILKLLRCGINVGIVTAAGYDEAGTY
ENRLKGLIVALHDSTDIPVSQKQNLTIMGGESSYLFRYYEDPEEDNFGFRQIDKEEWL
LPRMKAWSLEDVEKTLDFAERTLNRLRKRLNLPSEISIIRKVRAVGIVPGERYDEASK
RQVPVKLDREQLEEIVLTLQNTLESFAPSRRIQFSCFDGGSDVWCDIGGKDLGVRSLQ
QFYNPESPIQPSETLHVGDQFAPVGSANDFKARLAGCTLWIASPQETVNYLHRLLETD
"
gene complement(<628360..>630540)
/gene="NFI1"
/locus_tag="YOR156C"
/gene_synonym="SIZ2"
/db_xref="GeneID:854327"
mRNA complement(<628360..>630540)
/gene="NFI1"
/locus_tag="YOR156C"
/gene_synonym="SIZ2"
/product="SUMO ligase NFI1"
/transcript_id="NM_001183575.1"
/db_xref="GeneID:854327"
CDS complement(628360..630540)
/gene="NFI1"
/locus_tag="YOR156C"
/gene_synonym="SIZ2"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:16204216]"
/experiment="EXISTENCE:direct assay:GO:0003690
double-stranded DNA binding [PMID:23122649]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0016925 protein
sumoylation [PMID:23443663|PMID:12761287]"
/experiment="EXISTENCE:direct assay:GO:0019789 SUMO
transferase activity [PMID:12761287]"
/experiment="EXISTENCE:genetic interaction:GO:0016925
protein sumoylation [PMID:26960795]"
/experiment="EXISTENCE:genetic interaction:GO:1990683 DNA
double-strand break attachment to nuclear envelope
[PMID:27056668]"
/experiment="EXISTENCE:mutant phenotype:GO:0003690
double-stranded DNA binding [PMID:23122649]"
/experiment="EXISTENCE:mutant phenotype:GO:0007059
chromosome segregation [PMID:16204216]"
/experiment="EXISTENCE:mutant phenotype:GO:0016925 protein
sumoylation [PMID:22345352|PMID:23122649]"
/experiment="EXISTENCE:mutant phenotype:GO:0019789 SUMO
transferase activity [PMID:23122649]"
/note="SUMO E3 ligase; catalyzes sumoylation of Yku70p/80p
and Sir4p promoting telomere anchoring to the nuclear
envelope and regulating telomerase activity; DNA-bound
form catalyzes a DNA-damaged triggered sumoylation wave
resulting in multisite modification of several DNA repair
proteins, enhancing interactions between these proteins
and accelerating repair; sumoylates Cse4p, a prerequisite
for STUbL-mediated Ub-dependent degradation; role in
telomere length maintenance"
/codon_start=1
/product="SUMO ligase NFI1"
/protein_id="NP_014799.1"
/db_xref="GeneID:854327"
/db_xref="SGD:S000005682"
/translation="MASVMSNNNNNNNNNNASYMFTNPLSNTGGGLINEIKDAINEME
QLKVLELKQICKSLDLSITGKKAVLQDRIKQFLRKSCDIGHIDPWRPKAIKILIAKVR
INSSLPKYSTLWETLKTGAFKHPVASGQLPVTALQSTALPPYSQQQALAYSFTSPFYK
PIVQIPDANKKLKQSAGRGCTKMKFKVSKSNHDLLKSNKSYKLYLFSGFSIPFIYETV
GHEAIDFPYPCELVFNGTKLEDNVKGLKKQNGTGNPANLTPYLKVPTEMNHLDLHYLN
IDKEYSISCFIVEVFSPEALLGKILKRPKIIKQATTAYIKRTLNEQDDDDIITTSTVL
SLQCPISCTRMKYPAKTDQCKHIQCFDALWFLHSQSQVPTWQCPICQHPIKFDQLKIS
EFVDNIIQNCNEDVEQVEISVDGSWKPIHNSSAVITDTVNQNHSVKNENQGTVKQEQD
YDSRNAFDTNLRNGSNHNEPEIISLDSSDDEAFIPASKSFPTHVNPRNDQLRADIFPS
ESEGSSDYNPNHTSTPKGSPTMDQDNYQDAFQMRSFLNQGATTNINDTPTNNSSINSF
VTATNGDSRIFYNRGPSTPLLPAVLQNLTNQTEAQRNPYGPNYNTTAQDRNLLGIEGD
LPPIPPVDPNSEAETELPTRTTSAAHLPPYIHVSTSGHGDDGKIRKRRHSNVSIYIPK
NPYATLMKRRPQANHAIMNKTLAQTNDFNTSAQDNSEVVDLTSD"
gene complement(<630966..>631751)
/gene="PUP1"
/locus_tag="YOR157C"
/db_xref="GeneID:854328"
mRNA complement(<630966..>631751)
/gene="PUP1"
/locus_tag="YOR157C"
/product="proteasome core particle subunit beta 2"
/transcript_id="NM_001183576.3"
/db_xref="GeneID:854328"
CDS complement(630966..631751)
/gene="PUP1"
/locus_tag="YOR157C"
/EC_number="3.4.25.1"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:26928762|PMID:18504300]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0010499 proteasomal
ubiquitin-independent protein catabolic process
[PMID:19162040]"
/experiment="EXISTENCE:direct assay:GO:0019774 proteasome
core complex, beta-subunit complex [PMID:9087403]"
/experiment="EXISTENCE:direct assay:GO:0034515 proteasome
storage granule [PMID:18504300]"
/experiment="EXISTENCE:direct assay:GO:0043161
proteasome-mediated ubiquitin-dependent protein catabolic
process [PMID:11545745|PMID:19029916]"
/experiment="EXISTENCE:mutant phenotype:GO:0004175
endopeptidase activity [PMID:9207060|PMID:9312134]"
/note="Beta 2 subunit of the 20S proteasome; endopeptidase
with trypsin-like activity that cleaves after basic
residues; synthesized as a proprotein before being
proteolytically processed for assembly into 20S particle;
human homolog is subunit Z"
/codon_start=1
/product="proteasome core particle subunit beta 2"
/protein_id="NP_014800.3"
/db_xref="GeneID:854328"
/db_xref="SGD:S000005683"
/translation="MAGLSFDNYQRNNFLAENSHTQPKATSTGTTIVGVKFNNGVVIA
ADTRSTQGPIVADKNCAKLHRISPKIWCAGAGTAADTEAVTQLIGSNIELHSLYTSRE
PRVVSALQMLKQHLFKYQGHIGAYLIVAGVDPTGSHLFSIHAHGSTDVGYYLSLGSGS
LAAMAVLESHWKQDLTKEEAIKLASDAIQAGIWNDLGSGSNVDVCVMEIGKDAEYLRN
YLTPNVREEKQKSYKFPRGTTAVLKESIVNICDIQEEQVDITA"
gene <632164..>633120
/gene="PET123"
/locus_tag="YOR158W"
/gene_synonym="mS26"
/db_xref="GeneID:854329"
mRNA <632164..>633120
/gene="PET123"
/locus_tag="YOR158W"
/gene_synonym="mS26"
/product="mitochondrial 37S ribosomal protein mS26 PET123"
/transcript_id="NM_001183577.1"
/db_xref="GeneID:854329"
CDS 632164..633120
/gene="PET123"
/locus_tag="YOR158W"
/gene_synonym="mS26"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:11278769]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:11278769]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005763
mitochondrial small ribosomal subunit [PMID:11278769]"
/note="Mitochondrial ribosomal protein of the small
subunit; PET123 exhibits genetic interactions with PET122,
which encodes a COX3 mRNA-specific translational
activator"
/codon_start=1
/product="mitochondrial 37S ribosomal protein mS26 PET123"
/protein_id="NP_014801.1"
/db_xref="GeneID:854329"
/db_xref="SGD:S000005684"
/translation="MGKGAAKYGFKSGVFPTTRSILKSPTTKQTDIINKVKSPKPKGV
LGIGYAKGVKHPKGSHRLSPKVNFIDVDNLIAKTVAEPQSIKSSNGSAQKVRLQKAEL
RRKFLIEAFRKEEARLLHKHEYLQKRTKELEKAKELELEKLNKEKSSDLTIMTLDKMM
SQPLLRNRSPEESELLKLKRNYNRSLLNFQAHKKKLNELLNLYHVANEFIVTESQLLK
KIDKVFNDETEEFTDAYDVTSNFTQFGNRKLLLSGNTTLQTQINNAIMGSLSNEKFFD
ISLVDSYLNKDLKNISNKIDSKLNPTSNGAGNNGNNNNTTNL"
gene complement(<633282..>633566)
/gene="SME1"
/locus_tag="YOR159C"
/db_xref="GeneID:854330"
mRNA complement(<633282..>633566)
/gene="SME1"
/locus_tag="YOR159C"
/product="mRNA splicing protein SME1"
/transcript_id="NM_001183578.3"
/db_xref="GeneID:854330"
CDS complement(633282..633566)
/gene="SME1"
/locus_tag="YOR159C"
/experiment="EXISTENCE:direct assay:GO:0005682 U5 snRNP
[PMID:11720285|PMID:11720284]"
/experiment="EXISTENCE:direct assay:GO:0005685 U1 snRNP
[PMID:9630245|PMID:9012791]"
/experiment="EXISTENCE:direct assay:GO:0046540 U4/U6 x U5
tri-snRNP complex [PMID:10449419|PMID:10377396]"
/experiment="EXISTENCE:direct assay:GO:0071004 U2-type
prespliceosome [PMID:16618970]"
/experiment="EXISTENCE:mutant phenotype:GO:0000398 mRNA
splicing, via spliceosome [PMID:8918241]"
/experiment="EXISTENCE:physical interaction:GO:1990935
splicing factor binding [PMID:9528767]"
/note="Core Sm protein Sm E; part of heteroheptameric
complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and
Smx2p) that is part of the spliceosomal U1, U2, U4, and U5
snRNPs; homolog of human Sm E"
/codon_start=1
/product="mRNA splicing protein SME1"
/protein_id="NP_014802.3"
/db_xref="GeneID:854330"
/db_xref="SGD:S000005685"
/translation="MSNKVKTKAMVPPINCIFNFLQQQTPVTIWLFEQIGIRIKGKIV
GFDEFMNVVIDEAVEIPVNSADGKEDVEKGTPLGKILLKGDNITLITSAD"
gene <633839..>636757
/gene="MTR10"
/locus_tag="YOR160W"
/gene_synonym="KAP111"
/db_xref="GeneID:854331"
mRNA <633839..>636757
/gene="MTR10"
/locus_tag="YOR160W"
/gene_synonym="KAP111"
/product="mRNA transport regulator MTR10"
/transcript_id="NM_001183579.1"
/db_xref="GeneID:854331"
CDS 633839..636757
/gene="MTR10"
/locus_tag="YOR160W"
/gene_synonym="KAP111"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9545233]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:9412460|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0006606 protein
import into nucleus [PMID:9545233|PMID:9412460]"
/experiment="EXISTENCE:direct assay:GO:0008139 nuclear
localization sequence binding [PMID:9545233]"
/experiment="EXISTENCE:direct assay:GO:0034399 nuclear
periphery [PMID:22842922]"
/experiment="EXISTENCE:mutant phenotype:GO:0006403 RNA
localization [PMID:12167699]"
/experiment="EXISTENCE:mutant phenotype:GO:0006404 RNA
import into nucleus [PMID:17409072|PMID:16040803]"
/experiment="EXISTENCE:mutant phenotype:GO:0006606 protein
import into nucleus [PMID:9545233|PMID:9412460]"
/experiment="EXISTENCE:mutant phenotype:GO:0035719 tRNA
import into nucleus [PMID:33074312]"
/experiment="EXISTENCE:mutant phenotype:GO:0051031 tRNA
transport [PMID:20032305|PMID:17409072]"
/experiment="EXISTENCE:mutant phenotype:GO:0061015 snRNA
import into nucleus [PMID:31189105]"
/note="Nuclear import receptor; mediates the nuclear
localization of proteins involved in mRNA-nucleus export;
promotes dissociation of mRNAs from the nucleus-cytoplasm
mRNA shuttling protein Npl3p; required for retrograde
import of mature tRNAs; relocalizes from cytoplasm to the
nuclear periphery upon DNA replication stress"
/codon_start=1
/product="mRNA transport regulator MTR10"
/protein_id="NP_014803.1"
/db_xref="GeneID:854331"
/db_xref="SGD:S000005686"
/translation="MDNLQVSDIETALQCISSTASQDDKNKALQFLEQFQRSTVAWSI
CNEILSKEDPTNALLELNIFAAQTLRNKVTYDLSQLENNLPQFKDSLLTLLLSHNQKL
IITQLNVALARLAIQFLEWQNPIFEIISLLNSSPSILLNFLRILPEETLDIASTSLTE
VEFNSRIHELIDPIAEDVLKFLVSCIDLLQNTDGNSSSSISLEQILRCLNSWSYEFPV
EQLLTVQPLINLVFETISNGNESDMEAFDSAIDCLCVILRESRDTTNEQLISALFHQL
MLLQEKLLPTLFTDHPLNDEYDDDLLEGMTRLFVEAGEAWSVVISKNPDFFKPMVLVL
LMLTCKNEDLDVVSYTFPFWFNFKQSLVLPRYQESRKAYSDIFVKLINGIITHLQYPS
GQFSSKEEEDKFKDFRYHMGDVLKDCTAVVGTSEALSQPLIRIKSAIENNNSWQIMEA
PLFSLRTMAKEISLTENTILPEIIKIICNLPEQAKIRYASTLVLGRYTEWTAKHPELL
EVQLQYIFNGFQLHEGSSDMQSIITASSHALMFFCSDCSKLLVGYIDQLINFFLNVQS
SIDIESQFELCQGLSAVINNQPEAKVSVIFQKLVDDNLRQIEALIPQWKANPTLLAPQ
IADKIDLLYALFEELKPRYNYPQQGSEPLLPRIEFIWKALRTLLVDAGAMTDSIIVER
VAKLLRRIFERFHVFCEPILPSVAEFLIQGYLTTGFGSYLWCSGSLIVIFGDDESFPI
SPSLKDAVWKFALSQCETFILNFNKFDKLQLNDYHEAIIDFFSLISDLIMFYPGAFLN
STELLGPVLNVALECVNKLDNYDAYICILRCLDDIISWGFKTPPISTVSIEIVPDEWR
KQVINEVVIAHGNQLILVLFIGLVTTFENTAHSDAISCIVKCLRILTEANNNDATICI
DWIYKVVEQLGQVTLNERDNLAKAVVEGLNSKDYRKVREGIRAFVGWYSRKNINSRFE
"
gene complement(<636939..>638558)
/gene="PNS1"
/locus_tag="YOR161C"
/db_xref="GeneID:854332"
mRNA complement(<636939..>638558)
/gene="PNS1"
/locus_tag="YOR161C"
/product="Pns1p"
/transcript_id="NM_001183580.3"
/db_xref="GeneID:854332"
CDS complement(636939..638558)
/gene="PNS1"
/locus_tag="YOR161C"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="hypothetical protein; has similarity to Torpedo
californica tCTL1p, which is postulated to be a choline
transporter, neither null mutation nor overexpression
affects choline transport"
/codon_start=1
/product="Pns1p"
/protein_id="NP_014804.3"
/db_xref="GeneID:854332"
/db_xref="SGD:S000005687"
/translation="MPLNEKYERPPQPPPAYDPNHRPPSSSENSAAANVNDGQTPYHF
RQDQYYNLNSKTSGAPIGSFDEAFPTENDNKPRWNDWPFTIFFLCTVGGFIAIAAITL
RAWSQTYSSTGSGIYDGVNTGTLNTNAAILLVFVCIIALVFSVLGLTLCRIFPKQFIY
CGMVINLVASLGTAIMYMSLRYWSAGIVFLVFTFMTAWCYWGMRSRIPLSVAVLKVVV
DAMKKCPQIFFVSFVGALVASAFGFLFSAVIVATYIKYDPNSSNGGCDVSGGSCSHSK
LIGVLVVVFFCGYYISEVIRNVIHCVISGVFGSWYYMSKSDQGMPRWPAFGALKRAMT
YSFGSICFGSLLVALIDLLRQILQMIRHDVTSSGGGQIAIQILFMVFDWIIGFLKWLA
EYFNHYAYSFIALYGKPYLRAAKETWYMLREKGMDALINDNLINIALGLFSMFASYMT
ALFTFLYLRFTSPQYNSNGAYNGALMAFSFVIALQICNIATEAIRSGTATFFVALGND
PEVFHHSYPHRFDEIFRAYPDVLRKLSHQNV"
gene complement(<639121..>639267)
/locus_tag="YOR161C-C"
/db_xref="GeneID:1466484"
mRNA complement(<639121..>639267)
/locus_tag="YOR161C-C"
/product="uncharacterized protein"
/transcript_id="NM_001184625.1"
/db_xref="GeneID:1466484"
CDS complement(639121..639267)
/locus_tag="YOR161C-C"
/note="hypothetical protein; identified by gene-trapping,
microarray-based expression analysis, and genome-wide
homology searching"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878172.1"
/db_xref="GeneID:1466484"
/db_xref="SGD:S000028712"
/translation="MSYTRVDHPTGKMACHLRQILASPLFFANYVLHAAIHYPSSDIR
GDIL"
gene complement(<639560..>641992)
/gene="YRR1"
/locus_tag="YOR162C"
/gene_synonym="PDR2"
/db_xref="GeneID:854333"
mRNA complement(<639560..>641992)
/gene="YRR1"
/locus_tag="YOR162C"
/gene_synonym="PDR2"
/product="Yrr1p"
/transcript_id="NM_001183581.3"
/db_xref="GeneID:854333"
CDS complement(639560..641992)
/gene="YRR1"
/locus_tag="YOR162C"
/gene_synonym="PDR2"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:11909958]"
/experiment="EXISTENCE:direct assay:GO:0000981 DNA-binding
transcription factor activity, RNA polymerase II-specific
[PMID:10052137|PMID:11134057]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006357 regulation
of transcription by RNA polymerase II [PMID:9767597]"
/experiment="EXISTENCE:direct assay:GO:0034599 cellular
response to oxidative stress [PMID:9767597]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:19111667]"
/experiment="EXISTENCE:direct assay:GO:0045944 positive
regulation of transcription by RNA polymerase II
[PMID:11134057]"
/experiment="EXISTENCE:genetic interaction:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:9767597]"
/experiment="EXISTENCE:genetic interaction:GO:0034599
cellular response to oxidative stress [PMID:9767597]"
/experiment="EXISTENCE:mutant phenotype:GO:0000981
DNA-binding transcription factor activity, RNA polymerase
II-specific [PMID:10052137]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:9767597]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress [PMID:9767597]"
/note="Zn2-Cys6 zinc-finger transcription factor;
activates genes involved in multidrug resistance; paralog
of Yrm1p, acting on an overlapping set of target genes;
YRR1 has a paralog, PDR8, that arose from the whole genome
duplication"
/codon_start=1
/product="Yrr1p"
/protein_id="NP_014805.3"
/db_xref="GeneID:854333"
/db_xref="SGD:S000005688"
/translation="MKRRSDALLGSFQATNVTPPSDNSNSTAGGANGSNSGTPTSTSG
KKRNKLIKSCGFCRRRKLRCDQQKPMCSTCISRNLTTCQYAEEFNKNIEKKATYGPYP
NADLLKKVEELENKIRILEAEKNTNSSASSMYTSPNFPPLGTSVGRGSTETSSPLPDG
VINPYADRYYLQSKHSGRSTLYGPTSMRTQIANSNWGFIEKYKQLWAKVKVERNKWKQ
NNQKTMCRELGLLDESDWQPDPLIKQICRFLPSYNKALSILDDFFNDGACNEINVILD
KAKVRRDFLDYFMPEKEVKAEGDRSIVYILSNPKKNYYKAAVILLILCLKYFHTDVPT
PIEKFFTLLKGASTAKVFYIERAQMLILFYYHRETYSFGGDGSDLVNINECLVTTVTT
IGLHLNIRETFKEHEVFMGSIESLENVWLMAIFIDYNISCNVGRPLLINKFYLDENQD
HCILNSKSKTYEGKLKRYLKLTRPMLLTLYDRDKFPDLKAYSKRIINFVEEELGPLGH
YTGENISEEVPLRESRILSMAVGLLLSFYALIHSVLKVRNIESKNNTFQLVLINFSII
VNTTIRCYRIDKALYPEKFEASNPHLPPHMALSMSLTAGLFSKTLVFFCSLIYFKLTL
FENGLCLSNDMEVGWSDLTKLTVPLDKDLSLGTAMSLYSSIFDRLFTVGNKELIRTMH
RSSQFVIELAIERTYRTILGNVIEFRKLTEETWLAQIKQELDPQSDNPSSEAKIVSDR
QRDLSLAVPTPTPSIIPMLPSPGETKNHAKSQSEIIQMLTDEFWANYNSGWEELINQS
EFSTLFDDYKDN"
gene <642741..>643307
/gene="DDP1"
/locus_tag="YOR163W"
/db_xref="GeneID:854334"
mRNA <642741..>643307
/gene="DDP1"
/locus_tag="YOR163W"
/product="polyphosphatase DDP1"
/transcript_id="NM_001183582.1"
/db_xref="GeneID:854334"
CDS 642741..643307
/gene="DDP1"
/locus_tag="YOR163W"
/EC_number="3.6.1.52"
/EC_number="3.6.1.60"
/EC_number="3.6.1.10"
/experiment="EXISTENCE:direct assay:GO:0000298
endopolyphosphatase activity
[PMID:21775424|PMID:31175919]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006798
polyphosphate catabolic process [PMID:21775424]"
/experiment="EXISTENCE:direct assay:GO:0008486
diphosphoinositol-polyphosphate diphosphatase activity
[PMID:10419486]"
/experiment="EXISTENCE:direct assay:GO:0015961 diadenosine
polyphosphate catabolic process
[PMID:9450008|PMID:10085096]"
/experiment="EXISTENCE:direct assay:GO:0034431
bis(5'-adenosyl)-hexaphosphatase activity
[PMID:9450008|PMID:10085096]"
/experiment="EXISTENCE:direct assay:GO:0034432
bis(5'-adenosyl)-pentaphosphatase activity
[PMID:9450008|PMID:10085096]"
/experiment="EXISTENCE:direct assay:GO:1990174
phosphodiesterase decapping endonuclease activity
[PMID:23353937]"
/note="Diadenosine and diphosphoinositol polyphosphate
phosphatase; hydrolyzes diphosphorylated inositol
polyphosphates and diadenosine polyphosphates; high
specificity for diadenosine hexa- and pentaphosphates;
contains endopolyphosphatase activity with a high affinity
for polyphosphates, an activity also observed for its
human DIPP homologs; possesses mRNA decapping activity;
nudix hydrolase family member; protein abundance increases
in response to DNA replication stress"
/codon_start=1
/product="polyphosphatase DDP1"
/protein_id="NP_014806.1"
/db_xref="GeneID:854334"
/db_xref="SGD:S000005689"
/translation="MGKTADNHGPVRSETAREGRENQVYSPVTGARLVAGCICLTPDK
KQVLMITSSAHKKRWIVPKGGVEKDEPNYETTAQRETWEEAGCIGKIVANLGTVEDMR
PPKDWNKDIKQFENSRKDSEVAKHPPRTEFHFYELEIENLLDKFPECHKRHRKLYSYT
EAKQNLIDAKRPELLEALNRSAIIKDDK"
gene complement(<643394..>644332)
/gene="GET4"
/locus_tag="YOR164C"
/gene_synonym="ENV8"
/db_xref="GeneID:854335"
mRNA complement(<643394..>644332)
/gene="GET4"
/locus_tag="YOR164C"
/gene_synonym="ENV8"
/product="protein GET4"
/transcript_id="NM_001183583.3"
/db_xref="GeneID:854335"
CDS complement(643394..644332)
/gene="GET4"
/locus_tag="YOR164C"
/gene_synonym="ENV8"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006620
post-translational protein targeting to endoplasmic
reticulum membrane [PMID:20850366]"
/experiment="EXISTENCE:direct assay:GO:0072380 TRC complex
[PMID:20850366]"
/experiment="EXISTENCE:genetic interaction:GO:0045048
protein insertion into ER membrane [PMID:19325107]"
/note="Protein involved in inserting tail-anchored
proteins into ER membranes; forms a complex with Mdy2p;
highly conserved across species and homologous to human
gene C7orf20"
/codon_start=1
/product="protein GET4"
/protein_id="NP_014807.3"
/db_xref="GeneID:854335"
/db_xref="SGD:S000005690"
/translation="MVPAESNAVQAKLAKTLQRFENKIKAGDYYEAHQTLRTIANRYV
RSKSYEHAIELISQGALSFLKAKQGGSGTDLIFYLLEVYDLAEVKVDDISVARLVRLI
AELDPSEPNLKDVITGMNNWSIKFSEYKFGDPYLHNTIGSKLLEGDFVYEAERYFMLG
THDSMIKYVDLLWDWLCQVDDIEDSTVAEFFSRLVFNYLFISNISFAHESKDIFLERF
IEKFHPKYEKIDKNGYEIVFFEDYSDLNFLQLLLITCQTKDKSYFLNLKNHYLDFSQA
YKSELEFLGQEYFNIVAPKQTNFLQDMMSGFLGGSK"
gene <644566..>646896
/gene="SEY1"
/locus_tag="YOR165W"
/db_xref="GeneID:854336"
mRNA <644566..>646896
/gene="SEY1"
/locus_tag="YOR165W"
/product="dynamin-like GTPase SEY1"
/transcript_id="NM_001183584.1"
/db_xref="GeneID:854336"
CDS 644566..646896
/gene="SEY1"
/locus_tag="YOR165W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762|PMID:19665976]"
/experiment="EXISTENCE:direct assay:GO:0005933 cellular
bud [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0016320 endoplasmic
reticulum membrane fusion [PMID:22508509]"
/experiment="EXISTENCE:direct assay:GO:0032541 cortical
endoplasmic reticulum [PMID:19665976]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0007029
endoplasmic reticulum organization
[PMID:22508509|PMID:19665976]"
/experiment="EXISTENCE:genetic interaction:GO:0016320
endoplasmic reticulum membrane fusion [PMID:22508509]"
/experiment="EXISTENCE:genetic interaction:GO:0061024
membrane organization [PMID:12427979]"
/experiment="EXISTENCE:mutant phenotype:GO:0003924 GTPase
activity [PMID:19665976]"
/experiment="EXISTENCE:mutant phenotype:GO:0016320
endoplasmic reticulum membrane fusion [PMID:22508509]"
/note="Dynamin-like GTPase that mediates homotypic ER
fusion; has a role in ER morphology; interacts physically
and genetically with Yop1p and Rtn1p; functional ortholog
of the human atlastin ATL1, defects in which cause a form
of the human disease hereditary spastic paraplegia;
homolog of Arabidopsis RHD3"
/codon_start=1
/product="dynamin-like GTPase SEY1"
/protein_id="NP_014808.1"
/db_xref="GeneID:854336"
/db_xref="SGD:S000005691"
/translation="MADRPAIQLIDEEKEFHQSALQYFQQCIGNRDVGLDYHVISVFG
SQSSGKSTLLNVLFNTNFDTMDAQVKRQQTTKGIWLAHTKQVNTTIEIDNDRPDIFVL
DVEGSDGSERGEDQDFERKAALFAIAVSEVLIVNMWEQQIGLYQGNNMALLKTVFEVN
LSLFGKNDNDHKVLLLFVIRDHVGVTPLSSLSDSVTRELEKIWTELSKPAGCEGSSLY
DYFDLKFVGLAHKLLQEDKFTQDVKKLGDSFVMKGTENYYFKPQYHHRLPLDGWTMYA
ENCWDQIERNKDLDLPTQQILVARFKTEEISNEALEEFISKYDESIAPLKGNLGSLTS
QLVKLKEECLTKYDEQASRYARNVYMEKREALNTKLNSHISGTINEFLESLMEKLWDD
LKLEVSSRDKATTSFVESVAAGKSKIEKEFNESMETFKKLGLLISNEEITCKFSDDIE
ERIKQLRDAELKAKIGRIKKNLVPELKDHVIHLLSHPSKKVWDDIMNDFESTIKDNIS
AYQVEKDKYDFKIGLSESENAKIYKNIRILAWRTLDTTVHDYLKIDTIVSILRDRFED
VFRYDAEGSPRLWKTEEEIDGAFRVAKEHALEVFEVLSLAVTSDNVEIIPDVPMAEEE
SGEDNEIYRDNEGVFHSRRFAHILTELQKENVLDQFRRQINITVLDSKRSIITTRTHI
PPWIYVLLAVLGWNEFVAVIRNPLFVTLTLILGATFFVIHKFGLWGPVVNVVQSAVGE
TRTAIKDKLRQFVVEDHEVKESFEMKDFSKNEQKEK"
gene complement(<647126..>648502)
/gene="SWT1"
/locus_tag="YOR166C"
/db_xref="GeneID:854337"
mRNA complement(<647126..>648502)
/gene="SWT1"
/locus_tag="YOR166C"
/product="mRNA-processing endoribonuclease"
/transcript_id="NM_001183585.3"
/db_xref="GeneID:854337"
CDS complement(647126..648502)
/gene="SWT1"
/locus_tag="YOR166C"
/experiment="EXISTENCE:direct assay:GO:0004521 RNA
endonuclease activity [PMID:19127978]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:19127978|PMID:17030511]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:19127978]"
/experiment="EXISTENCE:mutant phenotype:GO:0004521 RNA
endonuclease activity [PMID:19127978]"
/experiment="EXISTENCE:mutant phenotype:GO:0071032 nuclear
mRNA surveillance of mRNP export [PMID:19127978]"
/note="RNA endoribonuclease involved in perinuclear mRNP
quality control; involved in perinuclear mRNP quality
control via the turnover of aberrant, unprocessed
pre-mRNAs; interacts with subunits of THO/TREX, TREX-2,
and RNA polymerase II; contains a PIN (PilT N terminus)
domain"
/codon_start=1
/product="mRNA-processing endoribonuclease"
/protein_id="NP_014809.3"
/db_xref="GeneID:854337"
/db_xref="SGD:S000005692"
/translation="MTDEKRAFPKGNNHIRSETFNGSVSHKISESIKDIASLRPHGKY
TVQDIDNIIASTSSHENRGQSGDSNGCINHDEEGDIPMCDLNDESDVEMISEYLSSQR
EMEAQSVANYMPKINDDLPLLNPPTLKTAFVVDTNFIISHLNTLEKLRSLSSTYHHLI
IVPTTVIQELDGLKKSPDIARDNDDTTNQEHDRTIGTLARWGNDWIYKNLANLDSGLI
GQKLKQSLNPGSLKDDSILDCCLYFKEILNCFVILLSNDKNLCTKALTEDILTVSFRK
NMDAKIIAMRAYEENQLRFANLRDSTVNNFDQNVTSYAHIPGIETPPLQFDKVSQNVF
EQVKETIFFAIDHTLRKEYGEDIGFIDYNPDKLTTIENASNYIYLFWVSVFSELFTCS
KIKKNEWKSLPTVLKSKPTNLNDLRTFEQFWETVLHFLFSKFTNEEKQSLEKQIHEWK
TSINAIST"
gene complement(<648804..>649007)
/gene="RPS28A"
/locus_tag="YOR167C"
/gene_synonym="RPS33A"
/db_xref="GeneID:854338"
mRNA complement(<648804..>649007)
/gene="RPS28A"
/locus_tag="YOR167C"
/gene_synonym="RPS33A"
/product="40S ribosomal protein eS28 RPS28A"
/transcript_id="NM_001183586.1"
/db_xref="GeneID:854338"
CDS complement(648804..649007)
/gene="RPS28A"
/locus_tag="YOR167C"
/gene_synonym="RPS33A"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:3533916]"
/experiment="EXISTENCE:direct assay:GO:0022627 cytosolic
small ribosomal subunit [PMID:3533916]"
/experiment="EXISTENCE:genetic interaction:GO:0000054
ribosomal subunit export from nucleus [PMID:16246728]"
/experiment="EXISTENCE:mutant phenotype:GO:1900153
positive regulation of nuclear-transcribed mRNA catabolic
process, deadenylation-dependent decay [PMID:24492965]"
/note="Protein component of the small (40S) ribosomal
subunit; has an extraribosomal function in regulation of
RPS28B, in which Rps28Ap binds to a decapping complex via
Edc3p, which then binds to RPS28B mRNA leading to its
decapping and degradation; homologous to mammalian
ribosomal protein S28, no bacterial homolog; RPS28A has a
paralog, RPS28B, that arose from the whole genome
duplication"
/codon_start=1
/product="40S ribosomal protein eS28 RPS28A"
/protein_id="NP_014810.1"
/db_xref="GeneID:854338"
/db_xref="SGD:S000005693"
/translation="MDNKTPVTLAKVIKVLGRTGSRGGVTQVRVEFLEDTSRTIVRNV
KGPVRENDILVLMESEREARRLR"
gene <649303..>651732
/gene="GLN4"
/locus_tag="YOR168W"
/db_xref="GeneID:854339"
mRNA <649303..>651732
/gene="GLN4"
/locus_tag="YOR168W"
/product="glutamine--tRNA ligase"
/transcript_id="NM_001183587.3"
/db_xref="GeneID:854339"
CDS 649303..651732
/gene="GLN4"
/locus_tag="YOR168W"
/EC_number="6.1.1.18"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0004819
glutamine-tRNA ligase activity [PMID:2991203]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:15706032]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:15706032]"
/experiment="EXISTENCE:direct assay:GO:1990825
sequence-specific mRNA binding [PMID:34039240]"
/experiment="EXISTENCE:mutant phenotype:GO:0006425
glutaminyl-tRNA aminoacylation [PMID:2991203]"
/note="Glutamine tRNA synthetase; monomeric class I tRNA
synthetase that catalyzes the specific glutaminylation of
tRNA(Gln); N-terminal domain proposed to be involved in
enzyme-tRNA interactions"
/codon_start=1
/product="glutamine--tRNA ligase"
/protein_id="NP_014811.3"
/db_xref="GeneID:854339"
/db_xref="SGD:S000005694"
/translation="MSSVEELTQLFSQVGFEDKKVKEIVKNKKVSDSLYKLIKETPSD
YQWNKSTRALVHNLASFVKGTDLPKSELIVNGIINGDLKTSLQVDAAFKYVKANGEAS
TKMGMNENSGVGIEITEDQVRNYVMQYIQENKERILTERYKLVPGIFADVKNLKELKW
ADPRSFKPIIDQEVLKLLGPKDERDLIKKKTKNNEKKKTNSAKKSSDNSASSGPKRTM
FNEGFLGDLHKVGENPQAYPELMKEHLEVTGGKVRTRFPPEPNGYLHIGHSKAIMVNF
GYAKYHNGTCYLRFDDTNPEKEAPEYFESIKRMVSWLGFKPWKITYSSDYFDELYRLA
EVLIKNGKAYVCHCTAEEIKRGRGIKEDGTPGGERYACKHRDQSIEQNLQEFRDMRDG
KYKPGEAILRMKQDLNSPSPQMWDLIAYRVLNAPHPRTGTKWRIYPTYDFTHCLVDSM
ENITHSLCTTEFYLSRESYEWLCDQVHVFRPAQREYGRLNITGTVLSKRKIAQLVDEK
FVRGWDDPRLFTLEAIRRRGVPPGAILSFINTLGVTTSTTNIQVVRFESAVRKYLEDT
TPRLMFVLDPVEVVVDNLSDDYEELATIPYRPGTPEFGERTVPFTNKFYIERSDFSEN
VDDKEFFRLTPNQPVGLIKVSHTVSFKSLEKDEAGKIIRIHVNYDNKVEEGSKPKKPK
TYIQWVPISSKYNSPLRVTETRVYNQLFKSENPSSHPEGFLKDINPESEVVYKESVME
HNFGDVVKNSPWVVDSVKNSEFYVEEDKDSKEVCRFQAMRVGYFTLDKESTTSKVILN
RIVSLKDATSK"
gene complement(<652010..>653884)
/gene="LCB4"
/locus_tag="YOR171C"
/db_xref="GeneID:854342"
mRNA complement(<652010..>653884)
/gene="LCB4"
/locus_tag="YOR171C"
/product="sphinganine kinase LCB4"
/transcript_id="NM_001183590.1"
/db_xref="GeneID:854342"
CDS complement(652010..653884)
/gene="LCB4"
/locus_tag="YOR171C"
/EC_number="2.7.1.91"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:16141212|PMID:12493772]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:12459470]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16141212]"
/experiment="EXISTENCE:direct assay:GO:0006665
sphingolipid metabolic process [PMID:9677363]"
/experiment="EXISTENCE:direct assay:GO:0008481 sphingosine
kinase activity [PMID:9677363]"
/experiment="EXISTENCE:direct assay:GO:0032541 cortical
endoplasmic reticulum [PMID:17686782]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0019722
calcium-mediated signaling [PMID:11278643]"
/note="Sphingoid long-chain base kinase; responsible for
synthesis of long-chain base phosphates, which function as
signaling molecules, regulates synthesis of ceramide from
exogenous long-chain bases, localizes to the Golgi and
late endosomes; LCB4 has a paralog, LCB5, that arose from
the whole genome duplication"
/codon_start=1
/product="sphinganine kinase LCB4"
/protein_id="NP_014814.1"
/db_xref="GeneID:854342"
/db_xref="SGD:S000005697"
/translation="MVVQKKLRAILTDEGVLIKSQSHHMFNKHGQLRSGDSLSLLSCL
SCLDDGTLSSDGGSFDEDDSLELLPLNTTIPFNRILNAKYVNVGQKGFNNGKISSNPF
QTENLSSSSENDDVENHSLSNDKAPVSESQSFPKKDKWDTKTNTVKVSPDDSQDNSPS
LGIKDNQQLIELTFAVPKGHDVIPQKLTLLIDHVSRKSRANTGEENISSGTVEEILEK
SYENSKRNRSILVIINPHGGKGTAKNLFLTKARPILVESGCKIEIAYTKYARHAIDIA
KDLDISKYDTIACASGDGIPYEVINGLYRRPDRVDAFNKLAVTQLPCGSGNAMSISCH
WTNNPSYAALCLVKSIETRIDLMCCSQPSYMNEWPRLSFLSQTYGVIAESDINTEFIR
WMGPVRFNLGVAFNIIQGKKYPCEVFVKYAAKSKKELKVHFLENKDKNKGCLTFEPNP
SPNSSPDLLSKNNINNSTKDELSPNFLNEDNFKLKYPMTEPVPRDWEKMDSELTDNLT
IFYTGKMPYIAKDTKFFPAALPADGTIDLVITDARIPVTRMTPILLSLDKGSHVLEPE
VIHSKILAYKIIPKVESGLFSVDGEKFPLEPLQVEIMPMLCKTLLRNGRYIDTEFESM
"
gene <654210..>656570
/gene="YRM1"
/locus_tag="YOR172W"
/db_xref="GeneID:854343"
mRNA <654210..>656570
/gene="YRM1"
/locus_tag="YOR172W"
/product="Yrm1p"
/transcript_id="NM_001183591.3"
/db_xref="GeneID:854343"
CDS 654210..656570
/gene="YRM1"
/locus_tag="YOR172W"
/experiment="EXISTENCE:direct assay:GO:0000981 DNA-binding
transcription factor activity, RNA polymerase II-specific
[PMID:14512416]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006357 regulation
of transcription by RNA polymerase II [PMID:14512416]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:14512416]"
/note="Zinc finger transcription factor involved in
multidrug resistance; Zn(2)-Cys(6) zinc finger
transcription factor; activates genes involved in
multidrug resistance; paralog of Yrr1p, acting on an
overlapping set of target genes"
/codon_start=1
/product="Yrm1p"
/protein_id="NP_014815.3"
/db_xref="GeneID:854343"
/db_xref="SGD:S000005698"
/translation="MSKRGSLQDRASPSEETVKKAQKRRKPIKSCAFCRKRKLRCDQQ
KPMCSTCKTRGRSGCLYTEKFTHKIETKELFGSTPNIELLKRIEDLEKRLDDKELTEK
DVALSTSPFRNPYANFYYLQCKGSGRRIVYGPTSLRTHLSNDDNRFVNTYNQLWSKVK
IERNRWKARHKWTMKPETQLLEGPPLEKTGSDILQQVCNVLPSFEQSSKIITDFFNTE
LETNEVSEVLDKTKIINDFTSSFLPSDELLPNGERRIEKLLPSTKKNYYKIGVILMIL
CIRHFYKNTPEEIEKFLIMLTGLSTAKVYFIERAQFLLLKYYHRELIWACGDDSHMIS
LVDLLCSTAIMLGLHLNIREIYKNQENIVGSMESLENLWVWIILSDFNVSLNIGRCLA
INSSYFQVDECENGERLPKNTNNYSSTVFFDQSNSCMGKLKRFLRLARPMLDQIYDRS
AFPDLAENCKKLRNFVETEFHPISYYTDSELISKVPLCEIKVLAQVLNLLLTFYSLRY
LIYKEKSVVLENNILQTILVSFSLVINTTILCFNLDEKHFPEFFDHNCVHLPPFMALS
LVYTNFLFPRASTGFCAFLYHKLTLFEKGYYLSSNIKDQEVTDWDLSTLNIPLDKAMN
LLTAFKIHSDIFAKWSNDNNKQLRIIMARSYTFVINIALESIYRAVLEKVIKYRTEVE
NTWLQQLQDELNGSYSLTDNVNTPIDPSLSDLGVTSASPLAGNSPGLPPEEVRNNSEN
ASHNNETGPIETELAQTISNEFWTAYNLGWEELMSQPDYKYLFDTQ"
rep_origin 656701..656772
/note="ARS1519; Autonomously Replicating Sequence"
/db_xref="SGD:S000118490"
gene <657264..>658325
/gene="DCS2"
/locus_tag="YOR173W"
/db_xref="GeneID:854344"
mRNA <657264..>658325
/gene="DCS2"
/locus_tag="YOR173W"
/product="5'-(N(7)-methyl 5'-triphosphoguanosine)-(mRNA)
diphosphatase"
/transcript_id="NM_001183592.1"
/db_xref="GeneID:854344"
CDS 657264..658325
/gene="DCS2"
/locus_tag="YOR173W"
/experiment="EXISTENCE:direct assay:GO:0000290
deadenylation-dependent decapping of nuclear-transcribed
mRNA [PMID:16963086]"
/experiment="EXISTENCE:direct assay:GO:0000932 P-body
[PMID:16963086]"
/experiment="EXISTENCE:direct assay:GO:0004857 enzyme
inhibitor activity [PMID:16963086]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0009267
cellular response to starvation [PMID:15240832]"
/note="m(7)GpppX pyrophosphatase regulator; non-essential,
stress induced regulatory protein; modulates
m7G-oligoribonucleotide metabolism; inhibits Dcs1p;
regulated by Msn2p, Msn4p, and the Ras-cAMP-cAPK signaling
pathway; mutant has increased aneuploidy tolerance; DCS2
has a paralog, DCS1, that arose from the whole genome
duplication"
/codon_start=1
/product="5'-(N(7)-methyl 5'-triphosphoguanosine)-(mRNA)
diphosphatase"
/protein_id="NP_014816.2"
/db_xref="GeneID:854344"
/db_xref="SGD:S000005699"
/translation="MGSQDLASLIGRFKYVRVLDSNPHTKVISLLGSIDGKDAVLTAE
KTHFIFDETVRRPSQSGRSTPIFFHREIDEYSFLNGITDLKELTSNDIYYWGLSVLKQ
HILHNPTAKVNLIWPASQFHIKGYDQQDLHVVRETPDMYRNIVVPFIQEMCTSERMKW
VNNILYEGAEDDRVVYKEYSSRNKEDGFVILPDMKWDGINIDSLYLVAIVYRDDIKSL
RDLNPNHRDWLIRLNKKIKTIIPQHYDYNVNPDELRVFIHYQPSYYHFHVHIVNIRHP
GVGEERGSGMTILLEDVIEALGFLGPEGYMKKTLTYVIGENHDLWKKGFKEEVEKQLK
HDGIATSPEKGSGFNTNLG"
gene <658747..>659601
/gene="MED4"
/locus_tag="YOR174W"
/db_xref="GeneID:854345"
mRNA <658747..>659601
/gene="MED4"
/locus_tag="YOR174W"
/product="Med4p"
/transcript_id="NM_001183593.3"
/db_xref="GeneID:854345"
CDS 658747..659601
/gene="MED4"
/locus_tag="YOR174W"
/experiment="EXISTENCE:direct assay:GO:0000979 RNA
polymerase II core promoter sequence-specific DNA binding
[PMID:23447536]"
/experiment="EXISTENCE:direct assay:GO:0006366
transcription by RNA polymerase II [PMID:9420330]"
/experiment="EXISTENCE:direct assay:GO:0034605 cellular
response to heat [PMID:23447536]"
/experiment="EXISTENCE:direct assay:GO:0070847 core
mediator complex [PMID:9891034]"
/note="Subunit of the RNA polymerase II mediator complex;
associates with core polymerase subunits to form the RNA
polymerase II holoenzyme; essential for transcriptional
regulation"
/codon_start=1
/product="Med4p"
/protein_id="NP_014817.3"
/db_xref="GeneID:854345"
/db_xref="SGD:S000005700"
/translation="MSVQDTKAVEFSMGHIRSSSVSLVAEATSNTNSEDKLSKVQLYE
DLCRYEDTLSKLVESVDRFKPNLDIAKDLIRTDEALFENVKLLAEYDNIYRNLQKIDK
DSEELDSKTRKILEILNECHDELKALPMLEQVEFEKNTILQQRSKINSTELLDYATKL
SKFTKIPPTFDKGAVGPNNFIWPAEDALRRGMLAMASLHSKELTRIPGEEVEETEVPT
VPPSQSEEQKGQMAKKEGTPKTDSFIFDGTAKEVGDEADNTKDKEKEENNDDALDLDL
DLFDPDDF"
gene complement(<659815..>661674)
/gene="ALE1"
/locus_tag="YOR175C"
/gene_synonym="LCA1; LPT1; SLC4"
/db_xref="GeneID:854346"
mRNA complement(<659815..>661674)
/gene="ALE1"
/locus_tag="YOR175C"
/gene_synonym="LCA1; LPT1; SLC4"
/product="lysophospholipid acyltransferase"
/transcript_id="NM_001183594.1"
/db_xref="GeneID:854346"
CDS complement(659815..661674)
/gene="ALE1"
/locus_tag="YOR175C"
/gene_synonym="LCA1; LPT1; SLC4"
/EC_number="2.3.1.23"
/EC_number="2.3.1.51"
/EC_number="2.3.1.n6"
/EC_number="2.3.1.n7"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762|PMID:17890783|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008374
O-acyltransferase activity [PMID:17890783]"
/experiment="EXISTENCE:direct assay:GO:0044233
mitochondria-associated endoplasmic reticulum membrane
contact site [PMID:17652094|PMID:38297966]"
/experiment="EXISTENCE:direct assay:GO:0071618
lysophosphatidylethanolamine acyltransferase activity
[PMID:17652094]"
/experiment="EXISTENCE:genetic interaction:GO:0003841
1-acylglycerol-3-phosphate O-acyltransferase activity
[PMID:17675291]"
/experiment="EXISTENCE:genetic interaction:GO:0046474
glycerophospholipid biosynthetic process [PMID:17675291]"
/experiment="EXISTENCE:genetic interaction:GO:0090640
phosphatidylcholine biosynthesis from
sn-glycero-3-phosphocholine [PMID:30514764]"
/experiment="EXISTENCE:mutant phenotype:GO:0003841
1-acylglycerol-3-phosphate O-acyltransferase activity
[PMID:17726007|PMID:17675291]"
/experiment="EXISTENCE:mutant phenotype:GO:0006646
phosphatidylethanolamine biosynthetic process
[PMID:17652094]"
/experiment="EXISTENCE:mutant phenotype:GO:0008374
O-acyltransferase activity [PMID:17890783]"
/experiment="EXISTENCE:mutant phenotype:GO:0036151
phosphatidylcholine acyl-chain remodeling [PMID:39730635]"
/experiment="EXISTENCE:mutant phenotype:GO:0046474
glycerophospholipid biosynthetic process
[PMID:17726007|PMID:17675291|PMID:17890783]"
/experiment="EXISTENCE:mutant phenotype:GO:0047184
1-acylglycerophosphocholine O-acyltransferase activity
[PMID:17726007|PMID:17951629]"
/experiment="EXISTENCE:mutant phenotype:GO:0071618
lysophosphatidylethanolamine acyltransferase activity
[PMID:17652094]"
/note="Broad-specificity lysophospholipid acyltransferase;
major lysophosphatidylethanolamine acyltransferase that
localizes to the mitochondria-associated ER membrane;
member of the MBOAT family of membrane-bound
O-acyltransferases; key component of Lands cycle; capable
of remodeling phosphatidylcholine containing VLC
polyunsaturated fatty acids and involved in fatty acid
exchange at the sn-2 position of mature
glycerophospholipids"
/codon_start=1
/product="lysophospholipid acyltransferase"
/protein_id="NP_014818.1"
/db_xref="GeneID:854346"
/db_xref="SGD:S000005701"
/translation="MYNPVDAVLTKIITNYGIDSFTLRYAICLLGSFPLNAILKRIPE
KRIGLKCCFIISMSMFYLFGVLNLVSGFRTLFISTMFTYLISRFYRSKFMPHLNFMFV
MGHLAINHIHAQFLNEQTQTTVDITSSQMVLAMKLTSFAWSYYDGSCTSESDFKDLTE
HQKSRAVRGHPPLLKFLAYAFFYSTLLTGPSFDYADFDSWLNCEMFRDLPESKKPMRR
HHPGERRQIPKNGKLALWKVVQGLAWMILSTLGMKHFPVKYVLDKDGFPTRSFIFRIH
YLFLLGFIHRFKYYAAWTISEGSCILCGLGYNGYDSKTQKIRWDRVRNIDIWTVETAQ
NTREMLEAWNMNTNKWLKYSVYLRVTKKGKKPGFRSTLFTFLTSAFWHGTRPGYYLTF
ATGALYQTCGKIYRRNFRPIFLREDGVTPLPSKKIYDLVGIYAIKLAFGYMVQPFIIL
DLKPSLMVWGSVYFYVHIIVAFSFFLFRGPYAKQVTEFFKSKQPKEIFIRKQKKLEKD
ISASSPNLGGILKAKIEHEKGKTAEEEEMNLGIPPIELEKWDNAKEDWEDFCKDYKEW
RNKNGLEIEEENLSKAFERFKQEFSNAASGSGERVRKMSFSGYSPKPISKKEE"
gene <662401..>663582
/gene="HEM15"
/locus_tag="YOR176W"
/db_xref="GeneID:854347"
mRNA <662401..>663582
/gene="HEM15"
/locus_tag="YOR176W"
/product="ferrochelatase HEM15"
/transcript_id="NM_001183595.3"
/db_xref="GeneID:854347"
CDS 662401..663582
/gene="HEM15"
/locus_tag="YOR176W"
/EC_number="4.98.1.1"
/experiment="EXISTENCE:direct assay:GO:0004325
ferrochelatase activity [PMID:1304908]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:7629135]"
/experiment="EXISTENCE:mutant phenotype:GO:0004325
ferrochelatase activity [PMID:2185242]"
/experiment="EXISTENCE:mutant phenotype:GO:0006783 heme
biosynthetic process [PMID:7035824]"
/note="Ferrochelatase; a mitochondrial inner membrane
protein, catalyzes insertion of ferrous iron into
protoporphyrin IX, the eighth and final step in the heme
biosynthetic pathway; human homolog FECH can complement
yeast mutant and allow growth of haploid null after
sporulation of a heterozygous diploid"
/codon_start=1
/product="ferrochelatase HEM15"
/protein_id="NP_014819.3"
/db_xref="GeneID:854347"
/db_xref="SGD:S000005702"
/translation="MLSRTIRTQGSFLRRSQLTITRSFSVTFNMQNAQKRSPTGIVLM
NMGGPSKVEETYDFLYQLFADNDLIPISAKYQKTIAKYIAKFRTPKIEKQYREIGGGS
PIRKWSEYQATEVCKILDKTCPETAPHKPYVAFRYAKPLTAETYKQMLKDGVKKAVAF
SQYPHFSYSTTGSSINELWRQIKALDSERSISWSVIDRWPTNEGLIKAFSENITKKLQ
EFPQPVRDKVVLLFSAHSLPMDVVNTGDAYPAEVAATVYNIMQKLKFKNPYRLVWQSQ
VGPKPWLGAQTAEIAEFLGPKVDGLMFIPIAFTSDHIETLHEIDLGVIGESEYKDKFK
RCESLNGNQTFIEGMADLVKSHLQSNQLYSNQLPLDFALGKSNDPVKDLSLVFGNHES
T"
gene complement(663812..663885)
/locus_tag="YNCO0021C"
/db_xref="GeneID:854348"
tRNA complement(663812..663885)
/locus_tag="YNCO0021C"
/product="tRNA-Val"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Valine tRNA (tRNA-Val), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:854348"
/db_xref="SGD:S000006766"
repeat_region complement(664003..664324)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007163"
repeat_region 664325..664469
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007172"
repeat_region 664813..665145
/note="Ty2 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007173"
gene complement(<665785..>667179)
/gene="MPC54"
/locus_tag="YOR177C"
/db_xref="GeneID:854349"
mRNA complement(<665785..>667179)
/gene="MPC54"
/locus_tag="YOR177C"
/product="Mpc54p"
/transcript_id="NM_001183596.1"
/db_xref="GeneID:854349"
CDS complement(665785..667179)
/gene="MPC54"
/locus_tag="YOR177C"
/experiment="EXISTENCE:direct assay:GO:0005198 structural
molecule activity [PMID:12796288]"
/experiment="EXISTENCE:direct assay:GO:0005816 spindle
pole body [PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0035974 meiotic
spindle pole body [PMID:10899120]"
/experiment="EXISTENCE:genetic interaction:GO:0032120
ascospore-type prospore membrane formation
[PMID:10899120]"
/experiment="EXISTENCE:mutant phenotype:GO:0005198
structural molecule activity [PMID:12796288]"
/experiment="EXISTENCE:mutant phenotype:GO:0032120
ascospore-type prospore membrane formation
[PMID:10899120]"
/experiment="EXISTENCE:mutant phenotype:GO:0048278 vesicle
docking [PMID:20826607]"
/note="Component of the meiotic outer plaque; a
membrane-organizing center which is assembled on the
cytoplasmic face of the spindle pole body during meiosis
II and triggers the formation of the prospore membrane;
potential Cdc28p substrate"
/codon_start=1
/product="Mpc54p"
/protein_id="NP_014820.1"
/db_xref="GeneID:854349"
/db_xref="SGD:S000005703"
/translation="MPEDTSYSNSFEDYYNNSHAISPYKDSFYKEMTPSKPNVRFGDD
DVNIFDQRKKVNEINKNNTVKRSIPSSISTTITPNKSSLKSPRGKRASKNSFDNETKL
ESKNETLKEVNDAVNRCYALCNIPTKHVSINSISDLAQTFETLAVGITHETNRKAECE
RSKNAIDSLYYHEQLEKKELNEKSLQMAIDHLLKVTKQNLRQADDGNKLKETEALKSF
IEEIEEVDDNKISINSLQQQLLEEKTANNILRRDYYKLQERGRRLCHEFQELQDDYSK
QMKQKEYEVQKLKNEIKVLLNMNDNLKAEKAHYSQKEKQYFQKYTYIEKYMNHVKEEY
NRKEDECKKLNFIIDKSMKKIEHLERSLQTQFTAQNSFSTAMIQEEGPKDAHLKDRYH
KVKEFMEQKLQTSKINDPSCSEAEALDNVLCLIESSMKTLDKNSKCYPIATKKCIKYV
TDSPRLKENEHVTN"
gene complement(<667860..>670241)
/gene="GAC1"
/locus_tag="YOR178C"
/db_xref="GeneID:854350"
mRNA complement(<667860..>670241)
/gene="GAC1"
/locus_tag="YOR178C"
/product="protein phosphatase regulator GAC1"
/transcript_id="NM_001183597.3"
/db_xref="GeneID:854350"
CDS complement(667860..670241)
/gene="GAC1"
/locus_tag="YOR178C"
/experiment="EXISTENCE:direct assay:GO:0000164 protein
phosphatase type 1 complex [PMID:8289829]"
/experiment="EXISTENCE:direct assay:GO:0031072 heat shock
protein binding [PMID:10207049]"
/experiment="EXISTENCE:genetic interaction:GO:0019888
protein phosphatase regulator activity [PMID:1310938]"
/experiment="EXISTENCE:mutant phenotype:GO:0007094 mitotic
spindle assembly checkpoint signaling [PMID:10072380]"
/experiment="EXISTENCE:mutant phenotype:GO:0009408
response to heat [PMID:10207049]"
/experiment="EXISTENCE:mutant phenotype:GO:0019888 protein
phosphatase regulator activity [PMID:11459182]"
/experiment="EXISTENCE:physical interaction:GO:0005977
glycogen metabolic process [PMID:8289829]"
/experiment="EXISTENCE:physical interaction:GO:0051321
meiotic cell cycle [PMID:8754819]"
/note="Regulatory subunit for Glc7p type-1 protein
phosphatase (PP1); tethers Glc7p to Gsy2p glycogen
synthase, binds Hsf1p heat shock transcription factor,
required for induction of some HSF-regulated genes under
heat shock; GAC1 has a paralog, PIG1, that arose from the
whole genome duplication"
/codon_start=1
/product="protein phosphatase regulator GAC1"
/protein_id="NP_014821.3"
/db_xref="GeneID:854350"
/db_xref="SGD:S000005704"
/translation="MVIQTATTLSPAKARPSFPHNDLIKSMSDSLISRPTHPPIRKLK
SSLKISHPEPISRSKSEIFCTSPEKNVRFAIELTTVKRFDKNAEPSSISNENSPTLSP
VDSNTAADDVQLFNNEDCWFNDSSLVTNLLKNEKKFRYMNSLNNMFKLDLYDSEDEDD
IDEHINSQAEYGYTYNSLSTRGKTSENKSATSSLATQATNICDWKLHCTDLVPFKIAP
PLFTKTLSASDLQGQLTKYLNGQNVKLHSLTQLGDDSSKITGLVYVKNLSFEKYLEIK
FTFNSWRDIHYVTANFNRTINSNVDEFKFTIDLNSLKYILLIKRIITMEKNTSSCPLN
IELCCRYDVNNETYYDNNNGKNYHLFMTTFKKGGETKEKIPVVVEPASQTDAAMSPKE
MKARFVSSNPTLSRFLPQSRKFSEDTDYYNTSPLKHLYHNDTTSWVKPKRLNVVLDKL
ENATPPPPSSALANDTARTGKITKDKNNVLAPPTASNSIDLPILGSQHQSLYSGSSSY
SSSSSSISSSLSFASSNNSSTNSSSASCSFPLTELDNFDYANLYEPNDTFTTANLFNH
SLNSLMPEISTPSFFGGFRNENTINNNDSKNLVTSLEDSYEDKQSVITDTTMDENNKT
STINNSTDTLIKPSKENGTVKENKSSANSTSAPSSSQNRASTILNDHSNGKSDLKYVN
YQSLLDSHCFYNHPSSPNLQSTSFSSAAPFSGISQASDIFDYENEDSDSNQIAGEIDN
NSFPPHFYLDEDDKSACLSDDALIDHHRNTNPFINTFSSSPPILSQEVDRWRL"
gene complement(<671845..>672411)
/gene="SYC1"
/locus_tag="YOR179C"
/db_xref="GeneID:854351"
mRNA complement(<671845..>672411)
/gene="SYC1"
/locus_tag="YOR179C"
/product="cleavage polyadenylation factor subunit SYC1"
/transcript_id="NM_001183598.1"
/db_xref="GeneID:854351"
CDS complement(671845..672411)
/gene="SYC1"
/locus_tag="YOR179C"
/experiment="EXISTENCE:curator inference:GO:0003723 RNA
binding [PMID:12819204]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005847 mRNA
cleavage and polyadenylation specificity factor complex
[PMID:12819204]"
/experiment="EXISTENCE:mutant phenotype:GO:0031441
negative regulation of mRNA 3'-end processing
[PMID:16431986]"
/note="Subunit of the APT subcomplex of cleavage and
polyadenylation factor; may have a role in 3' end
formation of both polyadenylated and non-polyadenylated
RNAs; SYC1 has a paralog, YSH1, that arose from the whole
genome duplication"
/codon_start=1
/product="cleavage polyadenylation factor subunit SYC1"
/protein_id="NP_014822.1"
/db_xref="GeneID:854351"
/db_xref="SGD:S000005705"
/translation="MDLPKDKSDRTHQRINLNNSGTDRTNDLYLHIVQTFGCIETTAT
ENATKLLMLGDVEVEISASSVSIEWTQKSMISQTIADSIVIMIIGLCASDKNVLSESE
LKERNHNVWKIQELQNLFREQFGDSFSIDEGIGKKENVKNGSVTIGKSKATIDFSTMK
LIDCNSNPLKGRVESILSIGQKLTTPLC"
gene complement(<674352..>675167)
/gene="DCI1"
/locus_tag="YOR180C"
/gene_synonym="ECI2"
/db_xref="GeneID:854352"
mRNA complement(<674352..>675167)
/gene="DCI1"
/locus_tag="YOR180C"
/gene_synonym="ECI2"
/product="putative dodecenoyl-CoA isomerase DCI1"
/transcript_id="NM_001183599.3"
/db_xref="GeneID:854352"
CDS complement(674352..675167)
/gene="DCI1"
/locus_tag="YOR180C"
/gene_synonym="ECI2"
/EC_number="5.3.3.21"
/experiment="EXISTENCE:direct assay:GO:0004165
delta(3)-delta(2)-enoyl-CoA isomerase activity
[PMID:10455114]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0005782 peroxisomal
matrix [PMID:10381339]"
/experiment="EXISTENCE:mutant phenotype:GO:0006635 fatty
acid beta-oxidation [PMID:10381339]"
/note="Peroxisomal protein; identification as a
delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in
fatty acid metabolism is disputed; DCI1 has a paralog,
ECI1, that arose from the whole genome duplication"
/codon_start=1
/product="putative dodecenoyl-CoA isomerase DCI1"
/protein_id="NP_014823.3"
/db_xref="GeneID:854352"
/db_xref="SGD:S000005706"
/translation="MSSRVCYHINGPFFIIKLIDPKHLNSLTFEDFVYIALLLHKAND
IDSVLFTVLQSSGKYFSSGGKFSAVNKLNDGDVTSEVEKVSKLVSAISSPNIFVANAF
AIHKKVLVCCLNGPAIGLSASLVALCDIVYSQNDSVFLLFPFSNLGFVAEVGTSVTLT
QKLGINSANEHMIFSTPVLFKELIGTIITKNYQLTNTETFNEKVLQDIKQNLEGLYPK
SVLGMKELLHSEMKQKLIKAQAMETNGTLPFWASGEPFKRFKQLQEGNRRHKL"
gene <675939..>677840
/gene="LAS17"
/locus_tag="YOR181W"
/gene_synonym="BEE1"
/db_xref="GeneID:854353"
mRNA <675939..>677840
/gene="LAS17"
/locus_tag="YOR181W"
/gene_synonym="BEE1"
/product="actin-binding protein LAS17"
/transcript_id="NM_001183600.1"
/db_xref="GeneID:854353"
CDS 675939..677840
/gene="LAS17"
/locus_tag="YOR181W"
/gene_synonym="BEE1"
/experiment="EXISTENCE:direct assay:GO:0003779 actin
binding [PMID:10512884|PMID:23290554]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0007015 actin
filament organization [PMID:9214384]"
/experiment="EXISTENCE:direct assay:GO:0030479 actin
cortical patch [PMID:10512884|PMID:16824951|PMID:9024694]"
/experiment="EXISTENCE:direct assay:GO:0032233 positive
regulation of actin filament bundle assembly
[PMID:19158382]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807]"
/experiment="EXISTENCE:direct assay:GO:0045010 actin
nucleation [PMID:23290554]"
/experiment="EXISTENCE:direct assay:GO:0071933 Arp2/3
complex binding [PMID:10322115]"
/experiment="EXISTENCE:direct assay:GO:0140224 SLAC
complex [PMID:22973053]"
/experiment="EXISTENCE:direct assay:GO:2000601 positive
regulation of Arp2/3 complex-mediated actin nucleation
[PMID:12499356|PMID:11604421|PMID:10322115|PMID:22973053|P
MID:16824951|PMID:12814545]"
/experiment="EXISTENCE:genetic interaction:GO:0051666
actin cortical patch localization [PMID:18177206]"
/experiment="EXISTENCE:mutant phenotype:GO:0003779 actin
binding [PMID:40374776|PMID:27637067]"
/experiment="EXISTENCE:mutant phenotype:GO:0030041 actin
filament polymerization [PMID:27637067]"
/experiment="EXISTENCE:mutant phenotype:GO:0045010 actin
nucleation [PMID:40374776]"
/experiment="EXISTENCE:mutant phenotype:GO:0051666 actin
cortical patch localization [PMID:18177206]"
/experiment="EXISTENCE:physical interaction:GO:0003779
actin binding [PMID:23290554]"
/experiment="EXISTENCE:physical interaction:GO:0140224
SLAC complex [PMID:22973053]"
/note="Actin assembly factor; C-terminal WCA domain
activates Arp2/3 complex-mediated nucleation of branched
actin filaments, polyproline domain able to nucleate actin
filaments in absence of Arp2/3; contains 3 binding sites
for monomeric actin which overlap with SH3 binding sites;
binding between SH3 and actin is competitive; mutants are
defective in endocytosis, bud site selection, cytokinesis;
human homolog WAS (Wiskott-Aldrich Syndrome) implicated in
severe immunodeficiency"
/codon_start=1
/product="actin-binding protein LAS17"
/protein_id="NP_014824.1"
/db_xref="GeneID:854353"
/db_xref="SGD:S000005707"
/translation="MGLLNSSDKEIIKRALPKASNKIIDVTVARLYIAYPDKNEWQYT
GLSGALALVDDLVGNTFFLKLVDINGHRGVIWDQELYVNFEYYQDRTFFHTFEMEECF
AGLLFVDINEASHFLKRVQKRERYANRKTLLNKNAVALTKKVREEQKSQVVHGPRGES
LIDNQRKRYNYEDVDTIPTTKHKAPPPPPPTAETFDSDQTSSFSDINSTTASAPTTPA
PALPPASPEVRKEETHPKHSLPPLPNQFAPLPDPPQHNSPPQNNAPSQPQSNPFPFPI
PEIPSTQSATNPFPFPVPQQQFNQAPSMGIPQQNRPLPQLPNRNNRPVPPPPPMRTTT
EGSGVRLPAPPPPPRRGPAPPPPPHRHVTSNTLNSAGGNSLLPQATGRRGPAPPPPPR
ASRPTPNVTMQQNPQQYNNSNRPFGYQTNSNMSSPPPPPVTTFNTLTPQMTAATGQPA
VPLPQNTQAPSQATNVPVAPPPPPASLGQSQIPQSAPSAPIPPTLPSTTSAAPPPPPA
FLTQQPQSGGAPAPPPPPQMPATSTSGGGSFAETTGDAGRDALLASIRGAGGIGALRK
VDKSQLDKPSVLLQEARGESASPPAAAGNGGTPGGPPASLADALAAALNKRKTKVGAH
DDMDNGDDW"
gene complement(<678191..>678793)
/gene="RPS30B"
/locus_tag="YOR182C"
/db_xref="GeneID:854354"
mRNA complement(join(<678191..678379,678791..>678793))
/gene="RPS30B"
/locus_tag="YOR182C"
/product="40S ribosomal protein eS30 RPS30B"
/transcript_id="NM_001183601.3"
/db_xref="GeneID:854354"
CDS complement(join(678191..678379,678791..678793))
/gene="RPS30B"
/locus_tag="YOR182C"
/experiment="EXISTENCE:mutant phenotype:GO:0006451
translational readthrough [PMID:39806023]"
/note="Protein component of the small (40S) ribosomal
subunit; homologous to mammalian ribosomal protein S30, no
bacterial homolog; RPS30B has a paralog, RPS30A, that
arose from the whole genome duplication; protein abundance
increases in response to DNA replication stress"
/codon_start=1
/product="40S ribosomal protein eS30 RPS30B"
/protein_id="NP_014825.3"
/db_xref="GeneID:854354"
/db_xref="SGD:S000005708"
/translation="MAKVHGSLARAGKVKSQTPKVEKTEKPKKPKGRAYKRLLYTRRF
VNVTLVNGKRRMNPGPSVQ"
gene <678872..>679261
/gene="FYV12"
/locus_tag="YOR183W"
/db_xref="GeneID:854355"
mRNA <678872..>679261
/gene="FYV12"
/locus_tag="YOR183W"
/product="Fyv12p"
/transcript_id="NM_001183602.1"
/db_xref="GeneID:854355"
CDS 678872..679261
/gene="FYV12"
/locus_tag="YOR183W"
/note="hypothetical protein; required for survival upon
exposure to K1 killer toxin"
/codon_start=1
/product="Fyv12p"
/protein_id="NP_014826.1"
/db_xref="GeneID:854355"
/db_xref="SGD:S000005709"
/translation="MRLLHHGEYGTKLIGGKCSIDGKLGHPCPLSRRRKKHLREKEMG
PQYVRMYGPKRKAIIRTGNPDDGINLPDTGRGTLTAATIWSRAYHSNYSYLVRPKVVT
LSKHRELMTTFLLYVLYVCIYISAFIK"
gene <679357..>680544
/gene="SER1"
/locus_tag="YOR184W"
/gene_synonym="ADE9"
/db_xref="GeneID:854356"
mRNA <679357..>680544
/gene="SER1"
/locus_tag="YOR184W"
/gene_synonym="ADE9"
/product="O-phospho-L-serine:2-oxoglutarate transaminase"
/transcript_id="NM_001183603.3"
/db_xref="GeneID:854356"
CDS 679357..680544
/gene="SER1"
/locus_tag="YOR184W"
/gene_synonym="ADE9"
/EC_number="2.6.1.52"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0004648
O-phospho-L-serine:2-oxoglutarate aminotransferase
activity [PMID:8017107]"
/experiment="EXISTENCE:mutant phenotype:GO:0006564
L-serine biosynthetic process [PMID:8017107|PMID:1326413]"
/experiment="EXISTENCE:mutant phenotype:GO:0009113 purine
nucleobase biosynthetic process [PMID:10509016]"
/note="3-phosphoserine aminotransferase; catalyzes the
formation of phosphoserine from 3-phosphohydroxypyruvate,
required for serine and glycine biosynthesis; regulated by
the general control of amino acid biosynthesis mediated by
Gcn4p; protein abundance increases in response to DNA
replication stress"
/codon_start=1
/product="O-phospho-L-serine:2-oxoglutarate transaminase"
/protein_id="NP_014827.3"
/db_xref="GeneID:854356"
/db_xref="SGD:S000005710"
/translation="MSLEREEPQHFGAGPAQMPTPVLQQAAKDLINFNDIGLGIGEIS
HRSKDATKVIEDSKKHLIELLNIPDTHEVFYLQGGGTTGFSSVATNLAAAYVGKHGKI
APAGYLVTGSWSQKSFEEAKRLHVPAEVIFNAKDYNNGKFGKIPDESLWEDKIKGKAF
SYVYLCENETVHGVEWPELPKCLVNDPNIEIVADLSSDILSRKIDVSQYGVIMAGAQK
NIGLAGLTLYIIKKSILKNISGASDETLHELGVPITPIAFDYPTVVKNNSAYNTIPIF
TLHVMDLVFQHILKKGGVEAQQAENEEKAKILYEALDANSDFYNVPVDPKCRSKMNVV
FTLKKDGLDDQFLKEAAARHLTGLKGHRSVGGFRASIYNALSVKAVQNLVDFIKEFAE
KNA"
gene complement(680685..680866)
/gene="SNR36"
/locus_tag="YNCO0022C"
/db_xref="GeneID:9164972"
ncRNA complement(680685..680866)
/ncRNA_class="snoRNA"
/gene="SNR36"
/locus_tag="YNCO0022C"
/product="SNR36"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:9182768]"
/experiment="EXISTENCE:mutant phenotype:GO:0030559 rRNA
pseudouridylation guide activity [PMID:9182768]"
/experiment="EXISTENCE:mutant phenotype:GO:0031118 rRNA
pseudouridine synthesis [PMID:9182768]"
/experiment="EXISTENCE:physical interaction:GO:0031429 box
H/ACA snoRNP complex [PMID:15923376]"
/note="H/ACA box small nucleolar RNA (snoRNA); guides
pseudouridylation of small subunit (SSU) rRNA at position
U1187"
/transcript_id="NR_132260.1"
/db_xref="GeneID:9164972"
/db_xref="SGD:S000007300"
gene complement(<681444..>682106)
/gene="GSP2"
/locus_tag="YOR185C"
/gene_synonym="CNR2"
/db_xref="GeneID:854357"
mRNA complement(<681444..>682106)
/gene="GSP2"
/locus_tag="YOR185C"
/gene_synonym="CNR2"
/product="Ran GTPase GSP2"
/transcript_id="NM_001183604.1"
/db_xref="GeneID:854357"
CDS complement(681444..682106)
/gene="GSP2"
/locus_tag="YOR185C"
/gene_synonym="CNR2"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0006997
nucleus organization [PMID:8455603]"
/note="GTP binding protein (mammalian Ranp homolog);
involved in the maintenance of nuclear organization, RNA
processing and transport; interacts with Kap121p, Kap123p
and Pdr6p (karyophilin betas); not required for viability;
protein abundance increases in response to DNA replication
stress; GSP2 has a paralog, GSP1, that arose from the
whole genome duplication"
/codon_start=1
/product="Ran GTPase GSP2"
/protein_id="NP_014828.1"
/db_xref="GeneID:854357"
/db_xref="SGD:S000005711"
/translation="MSAPAQNNAEVPTFKLVLVGDGGTGKTTFVKRHLTGEFEKKYIA
TIGVEVHPLSFYTNFGEIKFDVWDTAGQEKFGGLRDGYYINAQCAIIMFDVTSRITYK
NVPNWHRDLVRVCENIPIVLCGNKVDVKERKVKAKTITFHRKKNLQYYDISAKSNYNF
EKPFLWLARKLAGNPQLEFVASPALAPPEVQVDEQLMHQYQQEMDQATALPLPDEDDA
DL"
gene <683111..>683545
/locus_tag="YOR186W"
/db_xref="GeneID:854358"
mRNA <683111..>683545
/locus_tag="YOR186W"
/product="uncharacterized protein"
/transcript_id="NM_001183605.3"
/db_xref="GeneID:854358"
CDS 683111..683545
/locus_tag="YOR186W"
/note="hypothetical protein; proper regulation of
expression during heat stress is sphingolipid-dependent;
mCherry fusion protein localizes to the vacuole; YOR186W
has a paralog, YLR297W, that arose from the whole genome
duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014829.3"
/db_xref="GeneID:854358"
/db_xref="SGD:S000005712"
/translation="MTLAFTTFAISKINNSSTNEDSKVMILCDEHHPFEKGYFKSAIR
AFGNSIKLGLMGNSRPEDAASIFQDKNIPHDLTTEEFRLQLVCMAFSWFIFGLFIACL
LLCITLVLTSRYPGENENKATEVVPSSNIDDEEKQLSLSDMI"
gene <684030..>685343
/gene="TUF1"
/locus_tag="YOR187W"
/db_xref="GeneID:854359"
mRNA <684030..>685343
/gene="TUF1"
/locus_tag="YOR187W"
/product="translation elongation factor Tu"
/transcript_id="NM_001183606.1"
/db_xref="GeneID:854359"
CDS 684030..685343
/gene="TUF1"
/locus_tag="YOR187W"
/experiment="EXISTENCE:direct assay:GO:0003746 translation
elongation factor activity [PMID:3301847]"
/experiment="EXISTENCE:direct assay:GO:0003924 GTPase
activity [PMID:3301847]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:16823961|PMID:24769239|PMID:14562095|PMID:23727608]"
/experiment="EXISTENCE:direct assay:GO:0032543
mitochondrial translation [PMID:6353412]"
/experiment="EXISTENCE:genetic interaction:GO:0032543
mitochondrial translation [PMID:15695360|PMID:16777356]"
/experiment="EXISTENCE:mutant phenotype:GO:0070125
mitochondrial translational elongation [PMID:3905388]"
/note="Mitochondrial translation elongation factor Tu
(EF-Tu); involved in fundamental pathway of mtDNA
homeostasis; comprises both GTPase and guanine nucleotide
exchange factor activities, while these activities are
found in separate proteins in S. pombe and humans; rare
mutations in human mitochondrial elongation factor Tu
(EFTu) associated with severe lactic acidosis, rapidly
progressive fatal encephalopathy, severe infantile
macrocystic leukodystrophy with micropolygyria"
/codon_start=1
/product="translation elongation factor Tu"
/protein_id="NP_014830.1"
/db_xref="GeneID:854359"
/db_xref="SGD:S000005713"
/translation="MSALLPRLLTRTAFKASGKLLRLSSVISRTFSQTTTSYAAAFDR
SKPHVNIGTIGHVDHGKTTLTAAITKTLAAKGGANFLDYAAIDKAPEERARGITISTA
HVEYETAKRHYSHVDCPGHADYIKNMITGAAQMDGAIIVVAATDGQMPQTREHLLLAR
QVGVQHIVVFVNKVDTIDDPEMLELVEMEMRELLNEYGFDGDNAPIIMGSALCALEGR
QPEIGEQAIMKLLDAVDEYIPTPERDLNKPFLMPVEDIFSISGRGTVVTGRVERGNLK
KGEELEIVGHNSTPLKTTVTGIEMFRKELDSAMAGDNAGVLLRGIRRDQLKRGMVLAK
PGTVKAHTKILASLYILSKEEGGRHSGFGENYRPQMFIRTADVTVVMRFPKEVEDHSM
QVMPGDNVEMECDLIHPTPLEVGQRFNIREGGRTVGTGLITRIIE"
gene <685767..>689180
/gene="MSB1"
/locus_tag="YOR188W"
/db_xref="GeneID:854360"
mRNA <685767..>689180
/gene="MSB1"
/locus_tag="YOR188W"
/product="Msb1p"
/transcript_id="NM_001183607.3"
/db_xref="GeneID:854360"
CDS 685767..689180
/gene="MSB1"
/locus_tag="YOR188W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0030427 site of
polarized growth [PMID:23785492]"
/experiment="EXISTENCE:genetic interaction:GO:0030010
establishment of cell polarity [PMID:10679030]"
/note="RhoGAP-domain containing protein; identified as a
multicopy suppressor of ts alleles of CDC24, CDC42 and the
BEM4 null allele; involved in polarity establishment;
upstream regulator of 1,3-beta-glucan synthase and the
Pkc1p-MAPK pathway; proposed role promoting Cdc42p
function and inhibiting Rho1p function during early bud
development; localizes to sites of polarized growth;
potential Cdc28p substrate; relocalizes from the bud neck
to the cytoplasm upon DNA replication stress"
/codon_start=1
/product="Msb1p"
/protein_id="NP_014831.3"
/db_xref="GeneID:854360"
/db_xref="SGD:S000005714"
/translation="MNDMAKPLPTPPTAEIRKSRSNSPKKAQKTNLSPNKNQNNEKNV
PRSNGRTKNEHNSMDDEEFEFFHQFSREKVKGVIHVITAELKEKGPDVEFLMIPFRPE
QTNDKLLTLLNQLFPLGNGQPVNEKKQLRIVSKADVWTLFQCLKYIWCRLPNSEIIGW
KSYLEFKFREEDKKFPRKSFLEIMPQCLASPNHASIVYDFFDLIISISSNSRVNKMSA
RKISKMCAIWAFSKQIPNSDIQDYDFESAAMKSFAPNNSIQDGLDQWIPASDAMFHLL
LAFLRSFVPQDLESAKLPRTLKSLLFNNQYPPRKSTAYTSETILTIPLVTLKTDVFSR
KPWQLLERCNDLLDFSDHDAFEAREDYALLKSLFRKKNTVEGISRKMSQESRRLMKAM
STKHSTFQPGWAPRECIENISHLKECIEVKRLDIDDYFIWTWLSSLSFEQTSEKKKIF
GRSIILEFEFDGFKKWVVFQECDITLDYNKKGQLKKKTSAQSPTTEKELPPDDFELED
PPLSKSPTLSQTYKKFQAEVPQQSTVRRDSAPDNQGIYHTVISKNALTKNKHNVNLHS
FEHKISKWNPLNNLRKKSGSNSSSSSFEEKSKDAPIREEYHTNKNHKSKKEERVLSQF
STLNPDEYQLPVIETGSSNFKIEIPELMYEHDDDDSDKLKNSQKRATDSAIEELNGMV
EEMMINEPDDVKISITEAETFESLTKFDQYKPSNITDDDLQSSHSSAVHSLKLSTNTN
DSCADSSKYTADRKLAEPRKISEESKVNDDSSSYYSPNINNLPASRMPSQPTYSNSDS
KKAFTNESRLNVLQGAVSPSQQVTPKPYKNAPGDCVSPVQQKYYQNDRRNEMSPASAP
VPPSAYSPARSPQFSTNSAGFKQNTINVPVGYNDPAHVLANQPHMTYRDQHNYPSHQQ
KQRPFQNNIVPPELKSRNQRADASPIPQHMVPVKQGVPNLPSNVPLYQQMERMNPNHQ
HPVNTYKVTQPPYHNNTTNAYGNSRAGNAHMLDGKWSNNPPQMVPKGVRPNQYPQQHV
NRYSPQAQPVVPAEYYNGPPPMRAPPMMSHMVPAQEPIRYTAGANRRSFPQGMQQNAY
SVPAQPMGAVNSEFYLPEAPQGNKLHGNINKRQERKKLYDNIRSGNFGI"
gene <689624..>689974
/gene="IES4"
/locus_tag="YOR189W"
/db_xref="GeneID:854361"
mRNA <689624..>689974
/gene="IES4"
/locus_tag="YOR189W"
/product="Ies4p"
/transcript_id="NM_001183608.1"
/db_xref="GeneID:854361"
CDS 689624..689974
/gene="IES4"
/locus_tag="YOR189W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0031011 Ino80
complex [PMID:17693258]"
/experiment="EXISTENCE:genetic interaction:GO:0000722
telomere maintenance via recombination [PMID:23390378]"
/experiment="EXISTENCE:mutant phenotype:GO:0006974 DNA
damage response [PMID:17693258]"
/experiment="EXISTENCE:physical interaction:GO:0031011
Ino80 complex [PMID:24034245]"
/note="Component of the INO80 chromatin remodeling
complex; target of the Mec1p/Tel1p DNA damage signaling
pathway; proposed to link chromatin remodeling to
replication checkpoint responses"
/codon_start=1
/product="Ies4p"
/protein_id="NP_014832.1"
/db_xref="GeneID:854361"
/db_xref="SGD:S000005715"
/translation="MSQESSVLSESQEQLANNPKIEDTSPPSANSRDNSKPVLPWDYK
NKAIEIKSFSGYKVNFTGWIRRDVREERQRGSEFTASDVKGSDDKATRKKEPADEDPE
VKQLEKEGEDGLDS"
gene <690695..>692032
/gene="SPR1"
/locus_tag="YOR190W"
/gene_synonym="SSG1"
/db_xref="GeneID:854362"
mRNA <690695..>692032
/gene="SPR1"
/locus_tag="YOR190W"
/gene_synonym="SSG1"
/product="glucan 1,3-beta-glucosidase"
/transcript_id="NM_001183609.3"
/db_xref="GeneID:854362"
CDS 690695..692032
/gene="SPR1"
/locus_tag="YOR190W"
/gene_synonym="SSG1"
/EC_number="3.2.1.58"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0004338 glucan
exo-1,3-beta-glucosidase activity [PMID:7875558]"
/experiment="EXISTENCE:direct assay:GO:0005619 ascospore
wall [PMID:19779569]"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:24390141]"
/experiment="EXISTENCE:mutant phenotype:GO:0009277
fungal-type cell wall [PMID:8419289]"
/experiment="EXISTENCE:mutant phenotype:GO:0030437
ascospore formation [PMID:8419289]"
/note="Sporulation-specific exo-1,3-beta-glucanase;
contributes to ascospore thermoresistance; SPR1 has a
paralog, EXG1, that arose from the whole genome
duplication"
/codon_start=1
/product="glucan 1,3-beta-glucosidase"
/protein_id="NP_014833.3"
/db_xref="GeneID:854362"
/db_xref="SGD:S000005716"
/translation="MVSFRGLTTLTLLFTKLVNCNPVSTKNRDSIQFIYKEKDSIYSA
INNQAINEKIHGVNLGGWLVLEPYITPSLFETFRTNPYNDDGIPVDEYHFCEKLGYEK
AKERLYSHWSTFYKEEDFAKIASQGFNLVRIPIGYWAFTTLSHDPYVTAEQEYFLDRA
IDWARKYGLKVWIDLHGAAGSQNGFDNSGLRDSYKFLEDENLSATMKALTYILSKYST
DVYLDTVIGIELLNEPLGPVIDMERLKNLLLKPAYDYLRNKINSNQIIVIHDAFQPYH
YWDGFLNDEKNEYGVIIDHHHYQVFSQVELTRKMNERIKIACQWGKDAVSEKHWSVAG
EFSAALTDCTKWLNGVGLGARYDGSWTKDNEKSHYINTCANNENIALWPEERKQNTRK
FIEAQLDAFEMTGGWIMWCYKTENSIEWDVEKLIQLNIFPQPINDRKYPNQCH"
gene <692475..>697334
/gene="ULS1"
/locus_tag="YOR191W"
/gene_synonym="DIS1; RIS1; TID4"
/db_xref="GeneID:854363"
mRNA <692475..>697334
/gene="ULS1"
/locus_tag="YOR191W"
/gene_synonym="DIS1; RIS1; TID4"
/product="translocase ULS1"
/transcript_id="NM_001183610.1"
/db_xref="GeneID:854363"
CDS 692475..697334
/gene="ULS1"
/locus_tag="YOR191W"
/gene_synonym="DIS1; RIS1; TID4"
/experiment="EXISTENCE:direct assay:GO:0000776 kinetochore
[PMID:26906737]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:18603780|PMID:22842922|PMID:26906737]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:18603780]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:mutant phenotype:GO:0006325
chromatin organization [PMID:9271422]"
/experiment="EXISTENCE:mutant phenotype:GO:0007533 mating
type switching [PMID:9271422]"
/experiment="EXISTENCE:physical interaction:GO:0032183
SUMO binding [PMID:17728242]"
/note="Swi2/Snf2-related translocase, SUMO-Targeted
Ubiquitin Ligase (STUbL); required for maintenance of NHEJ
inhibition at telomeres; functions at telomeres to
translocate and ubiquitinylate poly-sumoylated Rap1p for
proteosomal degradation; plays role in antagonizing
silencing during mating-type switching; only known STUbL
with a translocase activity; contains RING finger domain;
relocalizes from nucleus to cytoplasm upon DNA replication
stress"
/codon_start=1
/product="translocase ULS1"
/protein_id="NP_014834.1"
/db_xref="GeneID:854363"
/db_xref="SGD:S000005717"
/translation="MAAVPTIDLTLADSDNEDIFHSFSSSTSVDKIDIRKENGKLRMA
GLEVAQSNDDAARQAFHVFKTNISNNETFDTILSKSKTITDSTFNNEKSSNEVKQQQV
LKEETMGSSNDEKKTQESSPSAEMIKLFYENDDVPLSDSFKQKEEGKRINQDEQVKEN
ICGISSSYVSKDYDGVEDDFEPNTCQDSNLDFQEEKLNLNNKPSQQQFSDPETKDNSL
KSENKDQIKGVTTTSYRDLPIESSAFQDSETQNNSKNTIPNIVNEKRTPALPSNLSSV
ESSLKNETAKVEGKTTVRLPGLQNNVALLEQEQSELFKHFSEQPVDISDFGRKIKRKH
SGDFADNKILKRPILPSKNMDHTTHNSHDSEQKNSSIIILSDEDESGAGINDIESPLK
VSEPNTADALRSSVPEVISLLDLPNIDLNNSVIKEASGSNSIPTSETDAQSSSSSVLQ
GTIMTEQATQSSQHECNSSLDTLKKNHQKLLKDLNSRESELRNALSCCKTNSEILRRK
LSRREKEVSDAEKHWQLLLTSMARGGRTISSTQQILVDEAENQLNKLKEKRQLTKSKL
DSINLKMYNYNEQWKSFVHSKNINLQKSLAALERSARDSKASATVNKRNECLAEKEKL
DQMLKEGTLSFSTYKQLTGEIQQKLNDLKLGDQRTTDINSVLPIVRQPLAKRDLFIKS
IDTAKDLLAKNTSRTEMTKRILYRHLDNLVSYKNFFEDGRSLIDINRRHVAHESAQIL
FTNGVKMPIVFETLQDYGIKFSNPAIVNPDRRAQYFKSIEVARDLISKSTRSEDAKRK
ITRFLNIIEEFRKDIDTGFPPTPLKREGVGKAVVGLRQQGLKMDRLYENLRRYKIPIT
SEELLQQSYLFPVNADQRPPSNWNIVENTEDTSSTANDLSMQDEFHISNMHAAEDQEQ
IRALLENVKQSESIIDGEALTPEDMTVNLLKHQRLGLHWLLQVENSAKKGGLLADDMG
LGKTIQAIALMLANRSEESKCKTNLIVAPVSVLRVWKGELETKVKKRAKFTTFIFGGS
GNGKVKHWRDLARYDAVLVSYQTLANEFKKHWPKKLDGEQNQLPAVPHIQALNRLKTS
NEYYSPFFCNDSTFYRILLDEGQNIKNKNTRASKACCTINGMYRWVLSGTPIQNSMDE
LYSLIRFLRIPPYHKEQRFKLDIGRFFQRNKQYQYDNEDRKNALRKVRVLLNAIMLRR
SKADKIDGKPLLELPPKIVEVDESRLKGEELKFYTALESKNQALAKKLLNNSTRGSYS
SVLTLLLRLRQACCHSELVVMGEKKAEGTKVANGKSFEDDWLRLYYKITHMSGEAQAQ
VITSMNSMTCFWCMEQLEPEAMSVLTGCGHLICDTCIEPFIEESSMLPQAKKTKGGAF
AIPCKDCQRLTNEKDIVSHKLYDQVINQGFTEEDLHAEYLSEMEKQKIQQKNVYVPNF
ESLEPSTKIEQCIQVIQRVFDESATEKIIIFSQFTTFFEILEHFLKNKLNFPYLKYIG
SMNAQRRSDVINEFYRDPEKRILLISMKAGNSGLTLTCANHVVIVDPFWNPYVEEQAQ
DRCYRISQTKKVQVHKLFIKDSVEDRISELQKRKKEMVDSAMDPGKIKEVNSLGRREL
GFLFGLNAL"
gene complement(<698768..>700567)
/gene="THI72"
/locus_tag="YOR192C"
/db_xref="GeneID:854364"
mRNA complement(<698768..>700567)
/gene="THI72"
/locus_tag="YOR192C"
/product="thiamine transporter"
/transcript_id="NM_001183611.3"
/db_xref="GeneID:854364"
CDS complement(698768..700567)
/gene="THI72"
/locus_tag="YOR192C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0015888
thiamine transport [PMID:16850348|PMID:9235906]"
/experiment="EXISTENCE:genetic interaction:GO:1903088
5-amino-1-ribofuranosylimidazole-4-carboxamide
transmembrane transport [PMID:24778186]"
/experiment="EXISTENCE:genetic interaction:GO:1903089
5-amino-1-ribofuranosylimidazole-4-carboxamide
transmembrane transporter activity [PMID:24778186]"
/note="Transporter of thiamine or related compound;
contributes to uptake of
5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside
(acadesine); shares sequence similarity with Thi7p"
/codon_start=1
/product="thiamine transporter"
/protein_id="NP_014835.3"
/db_xref="GeneID:854364"
/db_xref="SGD:S000005718"
/translation="MSFGTRISRALRFLEIPVKNRASVNFLRNPDLQPIKSVNQTWGF
WSNFAYWGVLSFNVGMWIGGSSALTVGLSYSETIGAFIIADLLTILFALANSCPGYDW
KVGFTLAQRFVFGIYGSALGIIIRILMSIVYYGSNAWLGGLCVNMILDSWSHHYLHLP
NTLSSKVAMTTKELIGFIIFHILTAFCYFMKPYHMNYILIWSCVGTFFAMLGMVIYLT
KSAHGVGDLFTSTHSTVTGSKKAWAWVYTISYWYGSVSPGCTNQSDFSRFGSSNCAIW
TGTIVALLIPATLIPVFGIIGASACEKLYGQTFWMPMDIFDNWLTTNYSAGARAATFF
CGFCFVMSQISYTISNCGFASGMDLAGLLPKYVDIKRGAIFAACVSWACLPWNFYNSS
STFLTVMSSFGVVMTPIITVMICDNFLIRKRQYSVTNAFVLKGEYYFTKGVNWRAIVA
WVCGMAPGLPGIAWEVNNDYFHNTGIINFFYGDSFFSFLISFFVYWGLCLLFPFKITV
KHDDKDYYGAFTDEEARKKGMVPYSEISEEEIRAYTLGECFTSGHEYKPESSDDELPE
LTKTSSENTKVFEIVHQKDNEKESSTSSEKQIA"
repeat_region complement(703429..703582)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007164"
repeat_region 703612..703762
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007174"
repeat_region 703772..704062
/note="Ty4 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007180"
gene complement(<703988..>704224)
/locus_tag="YOR192C-C"
/db_xref="GeneID:1466485"
mRNA complement(<703988..>704224)
/locus_tag="YOR192C-C"
/product="uncharacterized protein"
/transcript_id="NM_001184668.1"
/db_xref="GeneID:1466485"
CDS complement(703988..704224)
/locus_tag="YOR192C-C"
/note="hypothetical protein; identified by expression
profiling and mass spectrometry"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878173.1"
/db_xref="GeneID:1466485"
/db_xref="SGD:S000028857"
/translation="MMIIIFIELCRIADSLSWIPKSLRRTSSTFYIPNIIALLKMESQ
QLSQNSPTFQKHTPIGHINHDQYNSDSGSYYTLM"
mobile_element complement(704063..710023)
/note="YORCTy2-1; Ty2 element, LTR retrotransposon of the
Copia (Pseudoviridae) group; contains co-transcribed genes
TYA Gag and TYB Pol, encoding proteins involved in
structure and function of virus-like particles, flanked by
two direct repeats"
/mobile_element_type="retrotransposon:YORCTy2-1"
/db_xref="SGD:S000007168"
repeat_region complement(704063..704395)
/note="Ty2 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007165"
gene complement(<704419..>709732)
/locus_tag="YOR192C-B"
/db_xref="GeneID:854365"
mRNA complement(<704419..>709732)
/locus_tag="YOR192C-B"
/product="gag-pol fusion protein"
/transcript_id="NM_001184388.4"
/db_xref="GeneID:854365"
CDS complement(join(704419..708438,708440..709732))
/locus_tag="YOR192C-B"
/EC_number="2.7.7.7"
/EC_number="2.7.7.49"
/EC_number="3.1.26.4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9448009]"
/ribosomal_slippage
/note="Retrotransposon TYA Gag and TYB Pol genes;
transcribed/translated as one unit; polyprotein is
processed to make a nucleocapsid-like protein (Gag),
reverse transcriptase (RT), protease (PR), and integrase
(IN); similar to retroviral genes"
/codon_start=1
/product="gag-pol fusion protein"
/protein_id="NP_058185.3"
/db_xref="GeneID:854365"
/db_xref="SGD:S000007354"
/translation="MESQQLSQNSPTFHGSAYASVTSKEVPSNQDPLAVSASNLPEFD
RDSTKVNSQQETTPGTSAVPENHHHVSPQPASVPPPQNGQYQQHGMMTPNKAMASNWA
HYQQPSMMTCSHYQTSPAYYQPDPHYPLPQYIPPLSTSSPDPIGSQDQHSEVPQAKTK
VRNNVLPPHTLTSEENFSTWVKFYIRFLKNSNLGDIIPNDQGEIKRQMTYEEHAYIYN
TFQAFAPFHLLPTWVKQILEINYSDILTVLCKSVSKMQTNNQELKDWIALANLEYNGS
TSADTFEITVSTIIQRLKENNINVSDRLACQLILKGLSGDFKYLRNQYRTKTNMKLSQ
LFAEIQLIYDENKIMNLNKPSQYKQHSEYKNVSRTSPNTTNTKVTTRNYHRTNSSKPR
AAKAHNIATSSKFSRVNNDHINESTVSSQYLSDDNELSLGQQQKESKPTRTIDSNDEL
PDHLLIDSGASQTLVRSAHYLHHATPNSEINIVDAQKQDIPINAIGNLHFNFQNGTKT
SIKALHTPNIAYDLLSLSELANQNITACFTRNTLERSDGTVLAPIVKHGDFYWLSKKY
LIPSHISKLTINNVNKSKSVNKYPYPLIHRMLGHANFRSIQKSLKKNAVTYLKESDIE
WSNASTYQCPDCLIGKSTKHRHVKGSRLKYQESYEPFQYLHTDIFGPVHHLPKSAPSY
FISFTDEKTRFQWVYPLHDRREESILNVFTSILAFIKNQFNARVLVIQMDRGSEYTNK
TLHKFFTNRGITACYTTTADSRAHGVAERLNRTLLNDCRTLLHCSGLPNHLWFSAVEF
STIIRNSLVSPKNDKSARQHAGLAGLDITTILPFGQPVIVNNHNPDSKIHPRGIPGYA
LHPSRNSYGYIIYLPSLKKTVDTTNYVILQNKQTKLDQFDYDTLTFDDDLNRLTAHNQ
SFIEQNETEQSYDQNTESDHDYQSEIEINSDPLVNDFSSQSLNPLQLDKEPVQKVRAP
KEVDADISEYNILPSTIRSRTPHIINKESTEMGGTIESDTTSPRHSSTFTARNQKRPG
SPNDMIDLTSQDRVNYGLENIKTTRLGGTEEPYIQRNSDTNIKYRTTNSTPSIDDRSS
NSESTTPIISIETKAACDNTPSIDTDPPEYRSSDHATPNIMPDKSSKNVTADSILDDL
PLPDLTHQSPTDTSDVSKDIPHIHSRQTNSSLGGMDDSNVLTTTKSKKRSLEDNETEI
EVSRDTWNNKNMRSLEPPRSKKRINLIAAIKGVKSIKPVRTTLRYDEAITYNKDNKEK
DRYVEAYHKEISQLLKMNTWDTNKYYDRNDIDPKKVINSMFIFNKKRDGTHKARFVAR
GDIQHPDTYDSDMQSNTVHHYALMTSLSIALDNDYYITQLDISSAYLYADIKEELYIR
PPPHLGLNDKLLRLRKSLYGLKQSGANWYETIKSYLINCCDMQEVRGWSCVFKNSQVT
ICLFVDDMILFSKDLNANKKIITTLKKQYDTKIINLGEGDNEIQYDILGLEIKYQRSK
YMKLGMEKSLTEKLPKLNVPLNPKGKKLRAPGQPGHYIDQDELEIDEDEYKEKVHEMQ
KLIGLASYVGYKFRFDLLYYINTLAQHILFPSRQVLDMTYELIQFMWDTRDKQLIWHK
NKPTKPDNKLVAISDASYGNQPYYKSQIGNIFLLNGKVIGGKSTKASLTCTSTTEAEI
HAVSEAIPLLNNLSHLVQELNKKPIIKGLLTDSRSTISIIKSTNEEKFRNRFFGTKAM
RLRDEVSGNNLYVYYIETNKNIADVMTKPLPIKTFKLLTNKWIH"
gene complement(<708416..>709732)
/locus_tag="YOR192C-A"
/db_xref="GeneID:854366"
mRNA complement(<708416..>709732)
/locus_tag="YOR192C-A"
/product="gag protein"
/transcript_id="NM_001184387.1"
/db_xref="GeneID:854366"
CDS complement(708416..709732)
/locus_tag="YOR192C-A"
/note="Retrotransposon TYA Gag gene co-transcribed with
TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is
a nucleocapsid protein that is the structural constituent
of virus-like particles (VLPs); similar to retroviral Gag"
/codon_start=1
/product="gag protein"
/protein_id="NP_058186.1"
/db_xref="GeneID:854366"
/db_xref="SGD:S000007353"
/translation="MESQQLSQNSPTFHGSAYASVTSKEVPSNQDPLAVSASNLPEFD
RDSTKVNSQQETTPGTSAVPENHHHVSPQPASVPPPQNGQYQQHGMMTPNKAMASNWA
HYQQPSMMTCSHYQTSPAYYQPDPHYPLPQYIPPLSTSSPDPIGSQDQHSEVPQAKTK
VRNNVLPPHTLTSEENFSTWVKFYIRFLKNSNLGDIIPNDQGEIKRQMTYEEHAYIYN
TFQAFAPFHLLPTWVKQILEINYSDILTVLCKSVSKMQTNNQELKDWIALANLEYNGS
TSADTFEITVSTIIQRLKENNINVSDRLACQLILKGLSGDFKYLRNQYRTKTNMKLSQ
LFAEIQLIYDENKIMNLNKPSQYKQHSEYKNVSRTSPNTTNTKVTTRNYHRTNSSKPR
AAKAHNIATSSKFSRVNNDHINESTVSSQYLSDDNELSLRPATERI"
repeat_region complement(709691..710023)
/note="Ty2 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007166"
repeat_region 710024..710107
/note="Ty4 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007181"
gene 710201..710272
/gene="IMT1"
/locus_tag="YNCO0023W"
/db_xref="GeneID:854367"
tRNA 710201..710272
/gene="IMT1"
/locus_tag="YNCO0023W"
/product="tRNA-Met"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006413
translational initiation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0016282 eukaryotic
43S preinitiation complex [PMID:12008673]"
/experiment="EXISTENCE:direct assay:GO:0033290 eukaryotic
48S preinitiation complex [PMID:17242201]"
/experiment="EXISTENCE:direct assay:GO:0071074 eukaryotic
initiation factor eIF2 binding [PMID:14698289]"
/note="Methionine initiator tRNA (tRNA-Met); predicted by
tRNAscan-SE analysis; one of four initiator methionine
tRNAs in yeast that are functional for translation"
/db_xref="GeneID:854367"
/db_xref="SGD:S000006665"
gene <710446..>711576
/gene="PEX27"
/locus_tag="YOR193W"
/db_xref="GeneID:854368"
mRNA <710446..>711576
/gene="PEX27"
/locus_tag="YOR193W"
/product="Pex27p"
/transcript_id="NM_001183612.3"
/db_xref="GeneID:854368"
CDS 710446..711576
/gene="PEX27"
/locus_tag="YOR193W"
/experiment="EXISTENCE:direct assay:GO:0005778 peroxisomal
membrane [PMID:14517321]"
/experiment="EXISTENCE:genetic interaction:GO:0016559
peroxisome fission [PMID:36825558]"
/experiment="EXISTENCE:mutant phenotype:GO:0007031
peroxisome organization [PMID:14517321]"
/experiment="EXISTENCE:mutant phenotype:GO:0016559
peroxisome fission [PMID:36825558]"
/experiment="EXISTENCE:physical interaction:GO:0007031
peroxisome organization [PMID:14517321]"
/note="Peripheral peroxisomal membrane protein; required
for Vps1-dependent peroxisome fission but not
Dnm1-dependent fission; functions to control peroxisome
size and number; interacts with Vps1p and Pex25p;
accumulates at peroxisomal membrane constrictions; PEX27
has a paralog, PEX25, that arose from the whole genome
duplication"
/codon_start=1
/product="Pex27p"
/protein_id="NP_014836.3"
/db_xref="GeneID:854368"
/db_xref="SGD:S000005719"
/translation="MTSDPVNTNISSPTLTDRNADESWELLKREFNTLFSNLKTDSKE
EGNFTDNKGVIAKKPIVLQDNDDSDFTQNQGKVATATSTTSDRSFKRTLGSIEMKKRY
VKKNCQAKFVFNTLEGKEVCSKILQHTLGLLSLLLLTRKIRLLNFSSKLRLVIQQLSL
FRYYLRFGNFAINLYKIIKRFRWLREMKKLHYKDQSILFYFKNFRFFDIIEAFYNLTD
ELILFHKLQSMFGKKNTSHANTNRLMTFVKEQHYILWEVLNILAINKNIEQWRQLIRD
EIYLSIYNTSGNAIKEYELKYKLPTNDKVNLELRKNNITLDFYKIILNLLSNLINIKG
KRDKYNSELAYEIISVGSGVTELLKLWNRAKVTSANEHTSAV"
gene complement(<711683..>712543)
/gene="TOA1"
/locus_tag="YOR194C"
/db_xref="GeneID:854369"
mRNA complement(<711683..>712543)
/gene="TOA1"
/locus_tag="YOR194C"
/product="transcription initiation factor IIA large
subunit"
/transcript_id="NM_001183613.1"
/db_xref="GeneID:854369"
CDS complement(711683..712543)
/gene="TOA1"
/locus_tag="YOR194C"
/experiment="EXISTENCE:direct assay:GO:0003712
transcription coregulator activity [PMID:10567590]"
/experiment="EXISTENCE:direct assay:GO:0005672
transcription factor TFIIA complex [PMID:7862117]"
/experiment="EXISTENCE:direct assay:GO:0006367
transcription initiation at RNA polymerase II promoter
[PMID:7862117]"
/experiment="EXISTENCE:direct assay:GO:0017025 TBP-class
protein binding [PMID:7604282|PMID:10567590]"
/experiment="EXISTENCE:direct assay:GO:0051123 RNA
polymerase II preinitiation complex assembly
[PMID:10567590]"
/experiment="EXISTENCE:genetic interaction:GO:0003712
transcription coregulator activity [PMID:10567590]"
/experiment="EXISTENCE:genetic interaction:GO:0017025
TBP-class protein binding [PMID:10567590]"
/experiment="EXISTENCE:genetic interaction:GO:0051123 RNA
polymerase II preinitiation complex assembly
[PMID:10567590]"
/note="TFIIA large subunit; involved in transcriptional
activation, acts as antirepressor or as coactivator;
required, along with Toa2p, for ribosomal protein gene
transcription in vivo; homologous to largest and second
largest subunits of human and Drosophila TFIIA"
/codon_start=1
/product="transcription initiation factor IIA large
subunit"
/protein_id="NP_014837.1"
/db_xref="GeneID:854369"
/db_xref="SGD:S000005720"
/translation="MSNAEASRVYEIIVESVVNEVREDFENAGIDEQTLQDLKNIWQK
KLTETKVTTFSWDNQFNEGNINGVQNDLNFNLATPGVNSSEFNIKEENTGNEGLILPN
INSNNNIPHSGETNINTNTVEATNNSGATLNTNTSGNTNADVTSQPKIEVKPEIELTI
NNANITTVENIDDESEKKDDEEKEEDVEKTRKEKEQIEQVKLQAKKEKRSALLDTDEV
GSELDDSDDDYLISEGEEDGPDENLMLCLYDKVTRTKARWKCSLKDGVVTINRNDYTF
QKAQVEAEWV"
gene <712866..>715331
/gene="SLK19"
/locus_tag="YOR195W"
/db_xref="GeneID:854370"
mRNA <712866..>715331
/gene="SLK19"
/locus_tag="YOR195W"
/product="Slk19p"
/transcript_id="NM_001183614.3"
/db_xref="GeneID:854370"
CDS 712866..715331
/gene="SLK19"
/locus_tag="YOR195W"
/experiment="EXISTENCE:direct assay:GO:0000776 kinetochore
[PMID:15282802|PMID:10427094]"
/experiment="EXISTENCE:direct assay:GO:0005819 spindle
[PMID:11533655]"
/experiment="EXISTENCE:direct assay:GO:0051233 spindle
midzone [PMID:10427094]"
/experiment="EXISTENCE:direct assay:GO:0071459 protein
localization to chromosome, centromeric region
[PMID:30903360]"
/experiment="EXISTENCE:direct assay:GO:0098653 centromere
clustering [PMID:30903360]"
/experiment="EXISTENCE:genetic interaction:GO:0031536
positive regulation of exit from mitosis [PMID:14551257]"
/experiment="EXISTENCE:mutant phenotype:GO:0007052 mitotic
spindle organization [PMID:10427094|PMID:11533655]"
/experiment="EXISTENCE:mutant phenotype:GO:0007059
chromosome segregation [PMID:16443750]"
/experiment="EXISTENCE:mutant phenotype:GO:0007097 nuclear
migration [PMID:16443750]"
/experiment="EXISTENCE:mutant phenotype:GO:0031536
positive regulation of exit from mitosis
[PMID:27418100|PMID:14551257]"
/experiment="EXISTENCE:mutant phenotype:GO:0051321 meiotic
cell cycle [PMID:10835382]"
/experiment="EXISTENCE:mutant phenotype:GO:0098653
centromere clustering [PMID:23283988]"
/note="Kinetochore-associated protein; required for
chromosome segregation and kinetochore clustering;
required for normal segregation of chromosomes in meiosis
and mitosis; component of the FEAR regulatory network,
which promotes Cdc14p release from the nucleolus during
anaphase; potential Cdc28p substrate"
/codon_start=1
/product="Slk19p"
/protein_id="NP_014838.3"
/db_xref="GeneID:854370"
/db_xref="SGD:S000005721"
/translation="MNEVPTTPVRLILGQAQQREQNSENCSQERNPRTFNSEPDSSFN
SPGSSQFVIHPHEPLEKEKDEKQDLDRSIDYGRSSALNNKNNANPLENIDINKMFDDK
KSDSGTNDDKGGASTSDKHVLALNYSPIRVEMNSSEKRSDKNVDVDENDKEGSHINKK
LKLQLESVPDLKQSSTKDIINDKEEIMSSPMAIDMIETNISPNKFIINDGVERNDSFN
INTDTLKLENDINEKQQEEDFIKSNSNNVVNIDNAYKEKEDEENDITNSHINRLTPLY
ETSARESNSNEEGRNDYDDDNQLDIRHDNFQIVAKRNEELTDQIYHLNQMLNSLISKN
ESLSFQYEKLNKNHQLLIDLTNEKLDKLNTERESDIAKVEKFKKRIKELNTEIKVLNS
NQKILQEKFDASITEVNHIKGEHENTVNTLQQNEKILNDKNVELENMKAELKGNNDKL
SEYETTLNDLNSRIVQLNDKIESTDIVLKSKENELDNLKLSLKETLSISKDFNDSDLI
GQINELISTKNNLQQKMDDLNNLNDDNLKVVQDKLIKNEETLKLKEAEIDSLNSEMDE
LKKQITSKDDEFKMWQSKYETVEDEAKIRNAEVTELNGDIEDLKESKLHLEETITELE
NKVHKLENECELEKQKFEKTSLELESLQLKNSNIQAEHIKELENLHENLISLQNELKI
SSDRITTLTKENEVLMEQNNNNNNSVTLSNDQKDRDDEKIKSLGKQVQDWKEKYEAKE
KDTNKRLKLLAEDLYIQYSSKHEQKVKLLKKGYENKYQNKFDQLNLENKTLSEEIEQL
NKQLSSEREEKQELLKLLENEKK"
gene complement(<715593..>716837)
/gene="LIP5"
/locus_tag="YOR196C"
/db_xref="GeneID:854371"
mRNA complement(<715593..>716837)
/gene="LIP5"
/locus_tag="YOR196C"
/product="lipoate synthase"
/transcript_id="NM_001183615.1"
/db_xref="GeneID:854371"
CDS complement(715593..716837)
/gene="LIP5"
/locus_tag="YOR196C"
/EC_number="2.8.1.8"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:11914276|PMID:14576278|PMID:16823961|PMID:14562095|P
MID:24769239]"
/experiment="EXISTENCE:mutant phenotype:GO:0009249 protein
lipoylation [PMID:19570983]"
/note="Protein involved in biosynthesis of the coenzyme
lipoic acid; has similarity to E. coli lipoic acid
synthase"
/codon_start=1
/product="lipoate synthase"
/protein_id="NP_014839.1"
/db_xref="GeneID:854371"
/db_xref="SGD:S000005722"
/translation="MYRRSVGVLFVGRNTRWISSTIRCGTSATRPIRSNALNTDSDNA
SVRVPVGNSTEVENATSQLTGTSGKRRKGNRKRITEFKDALNLGPSFADFVSGKASKM
ILDPLEKARQNTEEAKKLPRWLKVPIPKGTNYHKLKGDVKELGLSTVCEEARCPNIGE
CWGGKDKSKATATIMLLGDTCTRGCRFCSVKTNRTPSKPDPMEPENTAEAIKRWGLGY
VVLTTVDRDDLVDGGANHLAETVRKIKQKAPNTLVETLSGDFRGDLKMVDIMAQCGLD
VYAHNLETVESLTPHVRDRRATYRQSLSVLERAKATVPSLITKTSIMLGLGETDEQIT
QTLKDLRNIQCDVVTFGQYMRPTKRHMKVVEYVKPEKFDYWKERALEMGFLYCASGPL
VRSSYKAGEAFIENVLKKRNMK"
gene <717086..>718384
/gene="MCA1"
/locus_tag="YOR197W"
/gene_synonym="YCA1"
/db_xref="GeneID:854372"
mRNA <717086..>718384
/gene="MCA1"
/locus_tag="YOR197W"
/gene_synonym="YCA1"
/product="Ca(2+)-dependent cysteine protease MCA1"
/transcript_id="NM_001183616.3"
/db_xref="GeneID:854372"
CDS 717086..718384
/gene="MCA1"
/locus_tag="YOR197W"
/gene_synonym="YCA1"
/experiment="EXISTENCE:direct assay:GO:0004198
calcium-dependent cysteine-type endopeptidase activity
[PMID:29363267|PMID:22761449]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12062425]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:24855027]"
/experiment="EXISTENCE:direct assay:GO:0006915 apoptotic
process [PMID:11983181]"
/experiment="EXISTENCE:mutant phenotype:GO:0004198
calcium-dependent cysteine-type endopeptidase activity
[PMID:22761449]"
/experiment="EXISTENCE:mutant phenotype:GO:0006515 protein
quality control for misfolded or incompletely synthesized
proteins [PMID:20624963]"
/experiment="EXISTENCE:mutant phenotype:GO:0006915
apoptotic process [PMID:16170310|PMID:16301538]"
/note="Ca2+-dependent cysteine protease; may cleave
specific substrates during the stress response; regulates
apoptosis upon H2O2 treatment; required for clearance of
insoluble protein aggregates during normal growth;
implicated in cell cycle dynamics and lifespan extension;
undergoes autocatalytic processing; similar to mammalian
metacaspases, but exists as a monomer due to an extra pair
of anti-parallel beta-strands that block potential
dimerization"
/codon_start=1
/product="Ca(2+)-dependent cysteine protease MCA1"
/protein_id="NP_014840.4"
/db_xref="GeneID:854372"
/db_xref="SGD:S000005723"
/translation="MYPGSGRYTYNNAGGNNGYQRPMAPPPNQQYGQQYGQQYEQQYG
QQYGQQNDQQFSQQYAPPPGPPPMAYNRPVYPPPQFQQEQAKAQLSNGYNNPNVNASN
MYGPPQNMSLPPPQTQTIQGTDQPYQYSQCTGRRKALIIGINYIGSKNQLRGCINDAH
NIFNFLTNGYGYSSDDIVILTDDQNDLVRVPTRANMIRAMQWLVKDAQPNDSLFLHYS
GHGGQTEDLDGDEEDGMDDVIYPVDFETQGPIIDDEMHDIMVKPLQQGVRLTALFDSC
HSGTVLDLPYTYSTKGIIKEPNIWKDVGQDGLQAAISYATGNRAALIGSLGSIFKTVK
GGMGNNVDRERVRQIKFSAADVVMLSGSKDNQTSADAVEDGQNTGAMSHAFIKVMTLQ
PQQSYLSLLQNMRKELAGKYSQKPQLSSSHPIDVNLQFIM"
gene complement(<718653..>720065)
/gene="BFR1"
/locus_tag="YOR198C"
/db_xref="GeneID:854373"
mRNA complement(<718653..>720065)
/gene="BFR1"
/locus_tag="YOR198C"
/product="Bfr1p"
/transcript_id="NM_001183617.1"
/db_xref="GeneID:854373"
CDS complement(718653..720065)
/gene="BFR1"
/locus_tag="YOR198C"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:11410665]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0042175 nuclear
outer membrane-endoplasmic reticulum membrane network
[PMID:11410665]"
/experiment="EXISTENCE:direct assay:GO:1990904
ribonucleoprotein complex [PMID:11410665]"
/experiment="EXISTENCE:mutant phenotype:GO:0003729 mRNA
binding [PMID:31455610]"
/experiment="EXISTENCE:mutant phenotype:GO:0005783
endoplasmic reticulum [PMID:31455610]"
/experiment="EXISTENCE:mutant phenotype:GO:0007088
regulation of mitotic nuclear division [PMID:8913743]"
/experiment="EXISTENCE:mutant phenotype:GO:0008298
intracellular mRNA localization [PMID:24424022]"
/experiment="EXISTENCE:mutant phenotype:GO:0035269 protein
O-linked glycosylation via mannose [PMID:31835530]"
/experiment="EXISTENCE:mutant phenotype:GO:0042149
cellular response to glucose starvation [PMID:24424022]"
/experiment="EXISTENCE:mutant phenotype:GO:0045048 protein
insertion into ER membrane [PMID:31455610]"
/experiment="EXISTENCE:mutant phenotype:GO:0051321 meiotic
cell cycle [PMID:8913743]"
/note="Component of mRNP complexes associated with
polyribosomes; involved in localization of mRNAs to P
bodies; implicated in secretion and nuclear segregation;
multicopy suppressor of BFA (Brefeldin A) sensitivity"
/codon_start=1
/product="Bfr1p"
/protein_id="NP_014841.1"
/db_xref="GeneID:854373"
/db_xref="SGD:S000005724"
/translation="MSSQQHKFKRPDVSVRDKKLDTLNVQLKKIDTEIGLIRKQIDQH
QVNDTTQQERKKLQDKNKEIIKIQADLKTRRSNIHDSIKQLDAQIKRKNNQIEEKLGK
KAKFSSTAEAKQRINEIEESIASGDLSLVQEKLLVKEMQSLNKLIKDLVNIEPIRKSV
DADKAKINQLKEELNGLNPKDVSNQFEENQQKLNDIHSKTQGVYDKRQTLFNKRAALY
KKRDELYSQIRQIRADFDNEFKSFRAKLDKERLKREEEQRLSKLLEQKDVDMGKLQEK
LTHAKIPAFTYEIGAIENSLLVLDPTYVKPKKNILPDLSSNALETKPARKVVADDLVL
VTPKKDDFVNVAPSKSKKYKKKNQQKNTENEQPASIFNKVDGKFTLEPTLIATLAELD
VTVPINSDDVKITVEQLKKKHEELLSKQEEQTKQNIESVEKEIEKLNLDYSNKEQQVK
KELEEKRLKEQEESEKDKEN"
gene complement(<720470..>721708)
/gene="MRM1"
/locus_tag="YOR201C"
/gene_synonym="PET56"
/db_xref="GeneID:854376"
mRNA complement(<720470..>721708)
/gene="MRM1"
/locus_tag="YOR201C"
/gene_synonym="PET56"
/product="Mrm1p"
/transcript_id="NM_001183620.3"
/db_xref="GeneID:854376"
CDS complement(720470..721708)
/gene="MRM1"
/locus_tag="YOR201C"
/gene_synonym="PET56"
/experiment="EXISTENCE:direct assay:GO:0000154 rRNA
modification [PMID:8643404]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:8643404]"
/experiment="EXISTENCE:direct assay:GO:0008989 rRNA
(guanine-N1-)-methyltransferase activity [PMID:8643404]"
/note="Ribose methyltransferase; modifies a functionally
critical, conserved nucleotide in mitochondrial 21S rRNA"
/codon_start=1
/product="Mrm1p"
/protein_id="NP_014844.3"
/db_xref="GeneID:854376"
/db_xref="SGD:S000005727"
/translation="MTSLTNAVFKRYLAVTPSAHQALKTRIKKKSSSFDKFFPQQSNS
RKKQWETLNEDKASWFKRKYAHVHAREQDRAADPYGKKKAHVEKLKEIKNQAKLNQKS
HKSKFQNKDIALKLMNDNPIFEYVYGTNSVYAALLNPSRNCHSRLLYHGTIPSKFLQI
VDELKVTTELVDKHRLNLLTNYGVHNNIALETKPLQPVEIAYLGDMDTSSAALSIHEL
GFNNENIPHELPYGTKTDAKKFPLGLYLDEITDPHNIGAIIRSAYFLGVDFIVMSRRN
CSPLTPVVSKTSSGALELLPIFYVDKPLEFFTKSQEMGGWTFITSHLANATSEKYTVG
KTISMHDLNGLCNELPVVLVVGNESQGVRTNLKMRSDFFVEIPFGGIEKGNRAPEPIV
DSLNVSVATALLIDNILTCK"
gene <721946..>722608
/gene="HIS3"
/locus_tag="YOR202W"
/gene_synonym="HIS10; HIS8"
/db_xref="GeneID:854377"
mRNA <721946..>722608
/gene="HIS3"
/locus_tag="YOR202W"
/gene_synonym="HIS10; HIS8"
/product="imidazoleglycerol-phosphate dehydratase HIS3"
/transcript_id="NM_001183621.1"
/db_xref="GeneID:854377"
CDS 721946..722608
/gene="HIS3"
/locus_tag="YOR202W"
/gene_synonym="HIS10; HIS8"
/EC_number="4.2.1.19"
/experiment="EXISTENCE:direct assay:GO:0000105 L-histidine
biosynthetic process [PMID:341150]"
/experiment="EXISTENCE:direct assay:GO:0004424
imidazoleglycerol-phosphate dehydratase activity
[PMID:341150]"
/experiment="EXISTENCE:mutant phenotype:GO:0000105
L-histidine biosynthetic process [PMID:14190241]"
/experiment="EXISTENCE:mutant phenotype:GO:0004424
imidazoleglycerol-phosphate dehydratase activity
[PMID:14190241]"
/note="Imidazoleglycerol-phosphate dehydratase; catalyzes
the sixth step in histidine biosynthesis; mutations cause
histidine auxotrophy and sensitivity to Cu, Co, and Ni
salts; transcription is regulated by general amino acid
control via Gcn4p"
/codon_start=1
/product="imidazoleglycerol-phosphate dehydratase HIS3"
/protein_id="NP_014845.1"
/db_xref="GeneID:854377"
/db_xref="SGD:S000005728"
/translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVA
EQATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQ
AFKEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMI
PHFLESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGV
LM"
gene <722911..>724725
/gene="DED1"
/locus_tag="YOR204W"
/gene_synonym="SPP81"
/db_xref="GeneID:854379"
mRNA <722911..>724725
/gene="DED1"
/locus_tag="YOR204W"
/gene_synonym="SPP81"
/product="DEAD-box ATP-dependent RNA helicase DED1"
/transcript_id="NM_001183623.3"
/db_xref="GeneID:854379"
CDS 722911..724725
/gene="DED1"
/locus_tag="YOR204W"
/gene_synonym="SPP81"
/EC_number="3.6.4.13"
/experiment="EXISTENCE:direct assay:GO:0000390
spliceosomal complex disassembly [PMID:16556937]"
/experiment="EXISTENCE:direct assay:GO:0002151
G-quadruplex RNA binding [PMID:30855040]"
/experiment="EXISTENCE:direct assay:GO:0003724 RNA
helicase activity [PMID:10364207|PMID:16216083]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:30281017|PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:25013175]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:25013175]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405|PMID:21925384]"
/experiment="EXISTENCE:direct assay:GO:0033592 RNA strand
annealing activity [PMID:16216083]"
/experiment="EXISTENCE:direct assay:GO:0051880
G-quadruplex DNA binding [PMID:30855040]"
/experiment="EXISTENCE:direct assay:GO:1901195 positive
regulation of formation of translation preinitiation
complex [PMID:30281017]"
/experiment="EXISTENCE:mutant phenotype:GO:0003724 RNA
helicase activity [PMID:10364207]"
/experiment="EXISTENCE:mutant phenotype:GO:0006413
translational initiation [PMID:14763975|PMID:9045610]"
/experiment="EXISTENCE:mutant phenotype:GO:1901195
positive regulation of formation of translation
preinitiation complex [PMID:30281017]"
/experiment="EXISTENCE:physical interaction:GO:0031370
eukaryotic initiation factor 4G binding [PMID:21925384]"
/note="ATP-dependent DEAD-box RNA helicase with
strand-annealing activity; promotes eIF4F-dependent 48S
translation preinitiation complex (PIC) assembly,
stimulating recruitment of mRNAs with long, structured
5'-UTRs; cooperates with Dbp1p in PIC attachment and
scanning; ATPase activity stimulated by mRNA
cap-associated factor binding; directly binds eIF4G; role
in spliceosomal complex disassembly; mutation in human
homolog DBY associated with male infertility; human
homolog DDX3X complements the null"
/codon_start=1
/product="DEAD-box ATP-dependent RNA helicase DED1"
/protein_id="NP_014847.3"
/db_xref="GeneID:854379"
/db_xref="SGD:S000005730"
/translation="MAELSEQVQNLSINDNNENGYVPPHLRGKPRSARNNSSNYNNNN
GGYNGGRGGGSFFSNNRRGGYGNGGFFGGNNGGSRSNGRSGGRWIDGKHVPAPRNEKA
EIAIFGVPEDPNFQSSGINFDNYDDIPVDASGKDVPEPITEFTSPPLDGLLLENIKLA
RFTKPTPVQKYSVPIVANGRDLMACAQTGSGKTGGFLFPVLSESFKTGPSPQPESQGS
FYQRKAYPTAVIMAPTRELATQIFDEAKKFTYRSWVKACVVYGGSPIGNQLREIERGC
DLLVATPGRLNDLLERGKISLANVKYLVLDEADRMLDMGFEPQIRHIVEDCDMTPVGE
RQTLMFSATFPADIQHLARDFLSDYIFLSVGRVGSTSENITQKVLYVENQDKKSALLD
LLSASTDGLTLIFVETKRMADQLTDFLIMQNFRATAIHGDRTQSERERALAAFRSGAA
TLLVATAVAARGLDIPNVTHVINYDLPSDVDDYVHRIGRTGRAGNTGLATAFFNSENS
NIVKGLHEILTEANQEVPSFLKDAMMSAPGSRSNSRRGGFGRNNNRDYRKAGGASAGG
WGSSRSRDNSFRGGSGWGSDSKSSGWGNSGGSNNSSWW"
gene complement(<725564..>727234)
/gene="GEP3"
/locus_tag="YOR205C"
/gene_synonym="AIM40; FMP38; MTG3"
/db_xref="GeneID:854380"
mRNA complement(<725564..>727234)
/gene="GEP3"
/locus_tag="YOR205C"
/gene_synonym="AIM40; FMP38; MTG3"
/product="Gep3p"
/transcript_id="NM_001183624.1"
/db_xref="GeneID:854380"
CDS complement(725564..727234)
/gene="GEP3"
/locus_tag="YOR205C"
/gene_synonym="AIM40; FMP38; MTG3"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:22621929]"
/experiment="EXISTENCE:direct assay:GO:0097177
mitochondrial ribosome binding [PMID:39180962]"
/experiment="EXISTENCE:mutant phenotype:GO:0030490
maturation of SSU-rRNA [PMID:22621929]"
/note="Protein required for mitochondrial ribosome small
subunit biogenesis; null mutant is defective in
respiration and in maturation of 15S rRNA; protein is
localized to the mitochondrial inner membrane; null mutant
interacts synthetically with prohibitin (Phb1p)"
/codon_start=1
/product="Gep3p"
/protein_id="NP_014848.1"
/db_xref="GeneID:854380"
/db_xref="SGD:S000005731"
/translation="MLNLCHALRGVRQFSCSVIVKVKCASCSIKLQDQDPSKPGYYTK
PKSLPDSKLNPDLQDLKYLLFSQDIQLSKQATQNDPDLKTKRDLLLRVICKRCSNALH
HNNYNPEEFPESTLNDILNYVPRGSNVMHIVPFVEFPLHLDPNVLKRNDLDTTLVLTK
SDQVFKDKNAVSKKVPIFMKQFLKNTLRIDSNKTFAISALKNWNISMFYNYFKNYTYL
LGNPNVGKSTLINTLLQKYLGYKVKIDSTGKINSPSEEVMQEAFTNPKNFFKIQAAGV
SHIPNLTRSVQAYQVGGKILFDLPGYSTSTSRLRLEELIDERWLQRLRKTDLFNRKHI
KQKTYESMKGTSQGGCYTVGGIFYLVPPKGSINQIVKYIPGPSKTFKNIEKGIDVFNS
CNSSSGTHPLSRYCGIKSVICEKSQYKRYAIPPFIGSIEIVLKDIGYILLRTTGRYEF
KGLHEIWIPRGIQVGIREPLENLIESGYQRYIETNGKESSCPRDRPIISSLYEMAPDE
ADTLNAVKKSYLEKTEKDLSARRFVDDDPYDLVQHLEKKKNPYWYYQW"
gene <727512..>729644
/gene="NOC2"
/locus_tag="YOR206W"
/db_xref="GeneID:854381"
mRNA <727512..>729644
/gene="NOC2"
/locus_tag="YOR206W"
/product="mRNA-binding ribosome synthesis protein NOC2"
/transcript_id="NM_001183625.3"
/db_xref="GeneID:854381"
CDS 727512..729644
/gene="NOC2"
/locus_tag="YOR206W"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10684247|PMID:11371346]"
/experiment="EXISTENCE:direct assay:GO:0005654 nucleoplasm
[PMID:11371346]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0030687
preribosome, large subunit precursor [PMID:23209026]"
/experiment="EXISTENCE:direct assay:GO:0030690 Noc1p-Noc2p
complex [PMID:11371346]"
/experiment="EXISTENCE:direct assay:GO:0030691 Noc2p-Noc3p
complex [PMID:11371346]"
/experiment="EXISTENCE:mutant phenotype:GO:0042273
ribosomal large subunit biogenesis
[PMID:11371346|PMID:23209026]"
/note="Protein involved in ribosome biogenesis; forms a
nucleolar complex with Mak21p that binds to 90S and 66S
pre-ribosomes; forms a nuclear complex with Noc3p that
binds to 66S pre-ribosomes; both complexes mediate
intranuclear transport of ribosomal precursors; acts as
part of a Mak21p-Noc2p-Rrp5p module that associates with
nascent pre-rRNA during transcription and has a role in
bigenesis of the large ribosomal subunit"
/codon_start=1
/product="mRNA-binding ribosome synthesis protein NOC2"
/protein_id="NP_014849.3"
/db_xref="GeneID:854381"
/db_xref="SGD:S000005732"
/translation="MGKVSKSTKKFQSKHLKHTLDQRRKEKIQKKRIQGRRGNKTDQE
KADAAGTREQQQLKKSAKEEVFKDMSVETFFEKGIEIPKENKKLKKKTTKEQSDEDSS
SSEEEEDMGQSMAKLAEKDPEFYKYLEENDKDLLDFAGTNPLDGIDSQDEGEDAERNS
NIEEKSEQMELEKEKIELSLKLVRKWKKQLHDSPSLKLLRNIISAFKVAVNLNKEENI
EDYKYAITDEKAFHELMFMVLKDVPQAIQKMAPYKIVKGARTLPNGGNVSRVSSIVKS
HAGSLLILLNDITNTETAALVLHSVNELMPYLLSYRRILKELIKSIVGVWSTTRELET
QIASFAFLINTTKEFKKSMLETTLKTTYSTFIKSCRKTNMRSMPLINFQKNSAAELFG
IDEVLGYQVGFEYIRQLAIHLRNTMNATTKKSSKINSAEAYKIVYNWQFCHSLDFWSR
VLSFACQPEKENGSESPLRQLIYPLVQVTLGVIRLIPTPQFFPLRFYLIKSLIRLSQN
SGVFIPIYPLLSEILTSTAFTKAPKKSPNLAAFDFEHNIKCTQAYLNTKIYQEGLSEQ
FVDLLGDYFALYCKNIAFPELVTPVIISLRRYIKTSTNVKLNKRLSTVVEKLNQNSTF
IQEKRSDVEFGPTNKSEVSRFLNDVAWNKTPLGSYVAVQREVKEEKARLMRESMEEQD
KERETEEAKLLNSLESDDDNEDVEMSDA"
rep_origin 729740..729969
/note="ARS1521; Autonomously Replicating Sequence"
/db_xref="SGD:S000118491"
gene complement(<730008..>733457)
/gene="RET1"
/locus_tag="YOR207C"
/gene_synonym="PDS2; RPC128; RPC2"
/db_xref="GeneID:854382"
mRNA complement(<730008..>733457)
/gene="RET1"
/locus_tag="YOR207C"
/gene_synonym="PDS2; RPC128; RPC2"
/product="DNA-directed RNA polymerase III core subunit
RET1"
/transcript_id="NM_001183626.1"
/db_xref="GeneID:854382"
CDS complement(730008..733457)
/gene="RET1"
/locus_tag="YOR207C"
/gene_synonym="PDS2; RPC128; RPC2"
/EC_number="2.7.7.6"
/experiment="EXISTENCE:direct assay:GO:0003899
DNA-directed RNA polymerase activity [PMID:3905793]"
/experiment="EXISTENCE:direct assay:GO:0005666 RNA
polymerase III complex [PMID:3905793|PMID:10611227]"
/experiment="EXISTENCE:direct assay:GO:0042797 tRNA
transcription by RNA polymerase III [PMID:3905793]"
/experiment="EXISTENCE:genetic interaction:GO:0042797 tRNA
transcription by RNA polymerase III [PMID:2199311]"
/note="Second-largest subunit of RNA polymerase III; RNA
polymerase III is responsible for the transcription of
tRNA and 5S RNA genes, and other low molecular weight
RNAs"
/codon_start=1
/product="DNA-directed RNA polymerase III core subunit
RET1"
/protein_id="NP_014850.1"
/db_xref="GeneID:854382"
/db_xref="SGD:S000005733"
/translation="MVAATKRRKTHIHKHVKDEAFDDLLKPVYKGKKLTDEINTAQDK
WHLLPAFLKVKGLVKQHLDSFNYFVDTDLKKIIKANQLILSDVDPEFYLKYVDIRVGK
KSSSSTKDYLTPPHECRLRDMTYSAPIYVDIEYTRGRNIIMHKDVEIGRMPIMLRSNK
CILYDADESKMAKLNECPLDPGGYFIVNGTEKVILVQEQLSKNRIIVEADEKKGIVQA
SVTSSTHERKSKTYVITKNGKIYLKHNSIAEEIPIAIVLKACGILSDLEIMQLVCGND
SSYQDIFAVNLEESSKLDIYTQQQALEYIGAKVKTMRRQKLTILQEGIEAIATTVIAH
LTVEALDFREKALYIAMMTRRVVMAMYNPKMIDDRDYVGNKRLELAGQLISLLFEDLF
KKFNNDFKLSIDKVLKKPNRAMEYDALLSINVHSNNITSGLNRAISTGNWSLKRFKME
RAGVTHVLSRLSYISALGMMTRISSQFEKSRKVSGPRALQPSQFGMLCTADTPEGEAC
GLVKNLALMTHITTDDEEEPIKKLCYVLGVEDITLIDSASLHLNYGVYLNGTLIGSIR
FPTKFVTQFRHLRRTGKVSEFISIYSNSHQMAVHIATDGGRICRPLIIVSDGQSRVKD
IHLRKLLDGELDFDDFLKLGLVEYLDVNEENDSYIALYEKDIVPSMTHLEIEPFTILG
AVAGLIPYPHHNQSPRNTYQCAMGKQAIGAIAYNQFKRIDTLLYLMTYPQQPMVKTKT
IELIDYDKLPAGQNATVAVMSYSGYDIEDALVLNKSSIDRGFGRCETRRKTTTVLKRY
ANHTQDIIGGMRVDENGDPIWQHQSLGPDGLGEVGMKVQSGQIYINKSVPTNSADAPN
PNNVNVQTQYREAPVIYRGPEPSHIDQVMMSVSDNDQALIKVLLRQNRRPELGDKFSS
RHGQKGVCGIIVKQEDMPFNDQGIVPDIIMNPHGFPSRMTVGKMIELISGKAGVLNGT
LEYGTCFGGSKLEDMSKILVDQGFNYSGKDMLYSGITGECLQAYIFFGPIYYQKLKHM
VLDKMHARARGPRAVLTRQPTEGRSRDGGLRLGEMERDCVIAYGASQLLLERLMISSD
AFEVDVCDKCGLMGYSGWCTTCKSAENIIKMTIPYAAKLLFQELLSMNIAPRLRLEDI
FQQ"
gene <733925..>736177
/gene="PTP2"
/locus_tag="YOR208W"
/db_xref="GeneID:854383"
mRNA <733925..>736177
/gene="PTP2"
/locus_tag="YOR208W"
/product="tyrosine protein phosphatase PTP2"
/transcript_id="NM_001183627.3"
/db_xref="GeneID:854383"
CDS 733925..736177
/gene="PTP2"
/locus_tag="YOR208W"
/EC_number="3.1.3.48"
/experiment="EXISTENCE:direct assay:GO:0004725 protein
tyrosine phosphatase activity [PMID:10523653]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10523653]"
/experiment="EXISTENCE:direct assay:GO:1903138 negative
regulation of cell integrity MAPK cascade [PMID:9224718]"
/experiment="EXISTENCE:genetic interaction:GO:0043937
regulation of sporulation [PMID:10679022]"
/experiment="EXISTENCE:genetic interaction:GO:0071852
fungal-type cell wall organization or biogenesis
[PMID:10523653]"
/experiment="EXISTENCE:mutant phenotype:GO:0051457
maintenance of protein location in nucleus
[PMID:10817757]"
/experiment="EXISTENCE:mutant phenotype:GO:0071474
cellular hyperosmotic response [PMID:10817757]"
/experiment="EXISTENCE:mutant phenotype:GO:0071507
pheromone response MAPK cascade [PMID:9224718]"
/experiment="EXISTENCE:mutant phenotype:GO:0071852
fungal-type cell wall organization or biogenesis
[PMID:10523653]"
/note="Phosphotyrosine-specific phosphatase; major role in
osmolarity sensing through dephosphorylation of the Hog1p
MAPK with a minor role by Ptp3p; inactivates and regulates
Hog1p localization; major role in the cell wall integrity
pathway through dephosphorylation of MAPK Slt2p with a
minor role by Ptp3p; minor role with Msg5p in the
pheromone adaptive response through dephosphorylation of
MAPK Fus3p with major role by Ptp3p; co-regulates the
calcium signaling pathway with Msg5p; nuclear localized"
/codon_start=1
/product="tyrosine protein phosphatase PTP2"
/protein_id="NP_014851.3"
/db_xref="GeneID:854383"
/db_xref="SGD:S000005734"
/translation="MDRIAQQYRNGKRDNNGNRMASSAISEKGHIQVNQTRTPGQMPV
YRGETINLSNLPQNQIKPCKDLDDVNIRRNNSNRHSKILLLDLCAGPNTNSFLGNTNA
KDITVLSLPLPSTLVKRSNYPFENLLKNYLGSDEKYIEFTKIIKDYDIFIFSDSFSRI
SSCLKTTFCLIEKFKKFICHFFPSPYLKFFLLEGSLNDSKAPSLGKNKKNCILPKLDL
NLNVNLTSRSTLNLRINIPPPNDSNKIFLQSLKKDLIHYSPNSLQKFFQFNMPADLAP
NDTILPNWLKFCSVKENEKVILKKLFNNFETLENFEMQRLEKCLKFKKKPLHQKQLSQ
KQRGPQSTDDSKLYSLTSLQRQYKSSLKSNIQKNQKLKLIIPKNNTSSSPSPLSSDDT
IMSPINDYELTEGIQSFTKNRYSNILPYEHSRVKLPHSPKPPAVSEASTTETKTDKSY
PMCPVDAKNHSCKPNDYINANYLKLTQINPDFKYIATQAPLPSTMDDFWKVITLNKVK
VIISLNSDDELNLRKWDIYWNNLSYSNHTIKLQNTWENICNINGCVLRVFQVKKTAPQ
NDNISQDCDLPHNGDLTSITMAVSEPFIVYQLQYKNWLDSCGVDMNDIIKLHKVKNSL
LFNPQSFITSLEKDVCKPDLIDDNNSELHLDTANSSPLLVHCSAGCGRTGVFVTLDFL
LSILSPTTNHSNKIDVWNMTQDLIFIIVNELRKQRISMVQNLTQYIACYEALLNYFAL
QKQIKNALPC"
gene complement(<736437..>737726)
/gene="NPT1"
/locus_tag="YOR209C"
/db_xref="GeneID:854384"
mRNA complement(<736437..>737726)
/gene="NPT1"
/locus_tag="YOR209C"
/product="nicotinate phosphoribosyltransferase"
/transcript_id="NM_001183628.1"
/db_xref="GeneID:854384"
CDS complement(736437..737726)
/gene="NPT1"
/locus_tag="YOR209C"
/EC_number="6.3.4.21"
/experiment="EXISTENCE:direct assay:GO:0004516 nicotinate
phosphoribosyltransferase activity [PMID:9521740]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11884393]"
/experiment="EXISTENCE:direct assay:GO:0019358 nicotinate
nucleotide salvage [PMID:11884393]"
/experiment="EXISTENCE:mutant phenotype:GO:0000183 rDNA
heterochromatin formation [PMID:11901108]"
/experiment="EXISTENCE:mutant phenotype:GO:0031509
subtelomeric heterochromatin formation [PMID:11901108]"
/note="Nicotinate phosphoribosyltransferase; acts in the
salvage pathway of NAD+ biosynthesis; required for
silencing at rDNA and telomeres and has a role in
silencing at mating-type loci; localized to the nucleus"
/codon_start=1
/product="nicotinate phosphoribosyltransferase"
/protein_id="NP_014852.1"
/db_xref="GeneID:854384"
/db_xref="SGD:S000005735"
/translation="MSEPVIKSLLDTDMYKITMHAAVFTNFPDVTVTYKYTNRSSQLT
FNKEAINWLKEQFSYLGNLRFTEEEIEYLKQEIPYLPSAYIKYISSSNYKLHPEEQIS
FTSEEIEGKPTHYKLKILVSGSWKDTILYEIPLLSLISEAYFKFVDIDWDYENQLEQA
EKKAETLFDNGIRFSEFGTRRRRSLKAQDLIMQGIMKAVNGNPDRNKSLLLGTSNILF
AKKYGVKPIGTVAHEWVMGVASISEDYLHANKNAMDCWINTFGAKNAGLALTDTFGTD
DFLKSFRPPYSDAYVGVRQDSGDPVEYTKKISHHYHDVLKLPKFSKIICYSDSLNVEK
AITYSHAAKENGMLATFGIGTNFTNDFRKKSEPQVKSEPLNIVIKLLEVNGNHAIKIS
DNLGKNMGDPATVKRVKEELGYTERSWSGDNEAHRWT"
gene <738320..>738532
/gene="RPB10"
/locus_tag="YOR210W"
/db_xref="GeneID:854385"
mRNA <738320..>738532
/gene="RPB10"
/locus_tag="YOR210W"
/product="DNA-directed RNA polymerase core subunit RPB10"
/transcript_id="NM_001183629.3"
/db_xref="GeneID:854385"
CDS 738320..738532
/gene="RPB10"
/locus_tag="YOR210W"
/experiment="EXISTENCE:direct assay:GO:0003899
DNA-directed RNA polymerase activity
[PMID:3905793|PMID:8288647|PMID:9837969]"
/experiment="EXISTENCE:direct assay:GO:0003968
RNA-directed RNA polymerase activity [PMID:18004386]"
/experiment="EXISTENCE:direct assay:GO:0005665 RNA
polymerase II, core complex [PMID:1748681|PMID:1331084]"
/experiment="EXISTENCE:direct assay:GO:0005666 RNA
polymerase III complex
[PMID:3905793|PMID:10611227|PMID:1748681]"
/experiment="EXISTENCE:direct assay:GO:0005736 RNA
polymerase I complex [PMID:9837969|PMID:1748681]"
/experiment="EXISTENCE:direct assay:GO:0006360
transcription by RNA polymerase I [PMID:9837969]"
/experiment="EXISTENCE:direct assay:GO:0008270 zinc ion
binding [PMID:1748681]"
/experiment="EXISTENCE:direct assay:GO:0042797 tRNA
transcription by RNA polymerase III [PMID:3905793]"
/experiment="EXISTENCE:genetic interaction:GO:0006360
transcription by RNA polymerase I [PMID:10085073]"
/experiment="EXISTENCE:mutant phenotype:GO:0006360
transcription by RNA polymerase I [PMID:10085073]"
/experiment="EXISTENCE:mutant phenotype:GO:0006366
transcription by RNA polymerase II [PMID:17179178]"
/note="RNA polymerase subunit ABC10-beta; common to RNA
polymerases I, II, and III"
/codon_start=1
/product="DNA-directed RNA polymerase core subunit RPB10"
/protein_id="NP_014853.3"
/db_xref="GeneID:854385"
/db_xref="SGD:S000005736"
/translation="MIVPVRCFSCGKVVGDKWESYLNLLQEDELDEGTALSRLGLKRY
CCRRMILTHVDLIEKFLRYNPLEKRD"
gene complement(<738924..>741569)
/gene="MGM1"
/locus_tag="YOR211C"
/gene_synonym="MNA1"
/db_xref="GeneID:854386"
mRNA complement(<738924..>741569)
/gene="MGM1"
/locus_tag="YOR211C"
/gene_synonym="MNA1"
/product="dynamin-related GTPase MGM1"
/transcript_id="NM_001183630.1"
/db_xref="GeneID:854386"
CDS complement(738924..741569)
/gene="MGM1"
/locus_tag="YOR211C"
/gene_synonym="MNA1"
/EC_number="3.6.5.5"
/experiment="EXISTENCE:direct assay:GO:0001786
phosphatidylserine binding [PMID:19236101]"
/experiment="EXISTENCE:direct assay:GO:0003924 GTPase
activity [PMID:19236101]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:27849155]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:19505460]"
/experiment="EXISTENCE:direct assay:GO:0005758
mitochondrial intermembrane space [PMID:11038181]"
/experiment="EXISTENCE:direct assay:GO:0030061
mitochondrial crista [PMID:19505460]"
/experiment="EXISTENCE:direct assay:GO:0070300
phosphatidic acid binding [PMID:19236101]"
/experiment="EXISTENCE:direct assay:GO:0080025
phosphatidylinositol-3,5-bisphosphate binding
[PMID:19236101]"
/experiment="EXISTENCE:direct assay:GO:0097002
mitochondrial inner boundary membrane
[PMID:27849155|PMID:19505460]"
/experiment="EXISTENCE:direct assay:GO:1901612 cardiolipin
binding [PMID:19236101]"
/experiment="EXISTENCE:mutant phenotype:GO:0007005
mitochondrion organization [PMID:15125685]"
/experiment="EXISTENCE:mutant phenotype:GO:0008053
mitochondrial fusion [PMID:12808034|PMID:17055438]"
/experiment="EXISTENCE:mutant phenotype:GO:0015886 heme
transport [PMID:32265272]"
/experiment="EXISTENCE:mutant phenotype:GO:0042407 cristae
formation [PMID:27849155]"
/note="Mitochondrial GTPase, present in complex with Ugo1p
and Fzo1p; required for mitochondrial morphology, fusion,
and genome maintenance; promotes membrane bending; plays a
direct role in formation and maintenance of lamellar, but
not of tubular, cristae; exists as long and short form
with different distributions; ratio of long to short forms
is regulated by Psd1p; homolog of human OPA1 involved in
autosomal dominant optic atrophy"
/codon_start=1
/product="dynamin-related GTPase MGM1"
/protein_id="NP_014854.2"
/db_xref="GeneID:854386"
/db_xref="SGD:S000005737"
/translation="MNASPVRLLILRRQLATHPAILYSSPYIKSPLVHLHSRMSNVHR
SAHANALSFVITRRSISHFPKIISKIIRLPIYVGGGMAAAGSYIAYKMEEASSFTKDK
LDRIKDLGESMKEKFNKMFSGDKSQDGGHGNDGTVPTATLIAATSLDDDESKRQGDPK
DDDDEDDDDEDDENDSVDTTQDEMLNLTKQMIEIRTILNKVDSSSAHLTLPSIVVIGS
QSSGKSSVLESIVGREFLPKGSNMVTRRPIELTLVNTPNSNNVTADFPSMRLYNIKDF
KEVKRMLMELNMAVPTSEAVSEEPIQLTIKSSRVPDLSLVDLPGYIQVEAADQPIELK
TKIRDLCEKYLTAPNIILAISAADVDLANSSALKASKAADPKGLRTIGVITKLDLVDP
EKARSILNNKKYPLSMGYVGVITKTPSSINRKHLGLFGEAPSSSLSGIFSKGQHGQSS
GEENTNGLKQIVSHQFEKAYFKENKKYFTNCQVSTKKLREKLIKILEISMSNALEPTS
TLIQQELDDTSYLFKVEFNDRHLTPKSYLLNNIDVLKLGIKEFQEKFHRNELKSILRA
ELDQKVLDVLATRYWKDDNLQDLSSSKLESDTDMLYWHKKLELASSGLTKMGIGRLST
MLTTNAILKELDNILESTQLKNHELIKDLVSNTAINVLNSKYYSTADQVENCIKPFKY
EIDLEERDWSLARQHSINLIKEELRQCNSRYQAIKNAVGSKKLANVMGYLENESNLQK
ETLGMSKLLLERGSEAIFLDKRCKVLSFRLKMLKNKCHSTIEKDRCPEVFLSAVSDKL
TSTAVLFLNVELLSDFFYNFPIELDRRLTLLGDEQVEMFAKEDPKISRHIELQKRKEL
LELALEKIDSILVFKKSYKGVSKNL"
gene <742910..>744181
/gene="STE4"
/locus_tag="YOR212W"
/gene_synonym="HMD2"
/db_xref="GeneID:854387"
mRNA <742910..>744181
/gene="STE4"
/locus_tag="YOR212W"
/gene_synonym="HMD2"
/product="G protein subunit beta"
/transcript_id="NM_001183631.3"
/db_xref="GeneID:854387"
CDS 742910..744181
/gene="STE4"
/locus_tag="YOR212W"
/gene_synonym="HMD2"
/experiment="EXISTENCE:direct assay:GO:0000749 response to
pheromone triggering conjugation with cellular fusion
[PMID:9428767]"
/experiment="EXISTENCE:direct assay:GO:0005834
heterotrimeric G-protein complex [PMID:8995254]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:8995254]"
/experiment="EXISTENCE:direct assay:GO:0005937 mating
projection [PMID:11073983]"
/experiment="EXISTENCE:direct assay:GO:0019901 protein
kinase binding [PMID:9428767]"
/experiment="EXISTENCE:direct assay:GO:0031680 G-protein
beta/gamma-subunit complex [PMID:8417317|PMID:8995254]"
/experiment="EXISTENCE:direct assay:GO:0120171
Cdc24p-Far1p-Gbetagamma complex [PMID:10087263]"
/experiment="EXISTENCE:genetic interaction:GO:0000750
pheromone-dependent signal transduction involved in
conjugation with cellular fusion [PMID:2107073]"
/experiment="EXISTENCE:genetic interaction:GO:0031680
G-protein beta/gamma-subunit complex [PMID:8417317]"
/experiment="EXISTENCE:mutant phenotype:GO:0000749
response to pheromone triggering conjugation with cellular
fusion [PMID:12660244|PMID:9732267|PMID:10087263]"
/experiment="EXISTENCE:mutant phenotype:GO:0000750
pheromone-dependent signal transduction involved in
conjugation with cellular fusion [PMID:2107073]"
/experiment="EXISTENCE:mutant phenotype:GO:0001403
invasive growth in response to glucose limitation
[PMID:15192700]"
/experiment="EXISTENCE:mutant phenotype:GO:0005834
heterotrimeric G-protein complex [PMID:2105453]"
/experiment="EXISTENCE:mutant phenotype:GO:0031267 small
GTPase binding [PMID:12660244]"
/experiment="EXISTENCE:mutant phenotype:GO:0043577
chemotropism [PMID:23613469|PMID:10087263]"
/experiment="EXISTENCE:mutant phenotype:GO:0061951
establishment of protein localization to plasma membrane
[PMID:9732267]"
/experiment="EXISTENCE:mutant phenotype:GO:1903260 protein
localization to mating projection tip [PMID:12660244]"
/experiment="EXISTENCE:physical interaction:GO:0000749
response to pheromone triggering conjugation with cellular
fusion [PMID:9428767]"
/experiment="EXISTENCE:physical interaction:GO:0001965
G-protein alpha-subunit binding [PMID:8417317]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:8649369]"
/experiment="EXISTENCE:physical interaction:GO:0019901
protein kinase binding [PMID:9428767]"
/experiment="EXISTENCE:physical interaction:GO:0031267
small GTPase binding [PMID:12660244]"
/experiment="EXISTENCE:physical interaction:GO:0031680
G-protein beta/gamma-subunit complex [PMID:8649369]"
/experiment="EXISTENCE:physical interaction:GO:0031682
G-protein gamma-subunit binding
[PMID:8417317|PMID:8649369]"
/experiment="EXISTENCE:physical interaction:GO:0097110
scaffold protein binding [PMID:7667635]"
/experiment="EXISTENCE:physical interaction:GO:0120171
Cdc24p-Far1p-Gbetagamma complex [PMID:10087263]"
/note="G protein beta subunit; forms a dimer with Ste18p
to activate mating signaling pathway, forms heterotrimer
with Gpa1p and Ste18p to dampen signaling;
pheromone-induced phosphorylation plays critical role in
chemotropism; may recruit Rho1p to polarized growth site
during mating; contains WD40 repeats"
/codon_start=1
/product="G protein subunit beta"
/protein_id="NP_014855.3"
/db_xref="GeneID:854387"
/db_xref="SGD:S000005738"
/translation="MAAHQMDSITYSNNVTQQYIQPQSLQDISAVEDEIQNKIEAARQ
ESKQLHAQINKAKHKIQDASLFQMANKVTSLTKNKINLKPNIVLKGHNNKISDFRWSR
DSKRILSASQDGFMLIWDSASGLKQNAIPLDSQWVLSCAISPSSTLVASAGLNNNCTI
YRVSKENRVAQNVASIFKGHTCYISDIEFTDNAHILTASGDMTCALWDIPKAKRVREY
SDHLGDVLALAIPEEPNSENSSNTFASCGSDGYTYIWDSRSPSAVQSFYVNDSDINAL
RFFKDGMSIVAGSDNGAINMYDLRSDCSIATFSLFRGYEERTPTPTYMAANMEYNTAQ
SPQTLKSTSSSYLDNQGVVSLDFSASGRLMYSCYTDIGCVVWDVLKGEIVGKLEGHGG
RVTGVRSSPDGLAVCTGSWDSTMKIWSPGYQ"
gene complement(<744535..>745281)
/gene="SAS5"
/locus_tag="YOR213C"
/db_xref="GeneID:854388"
mRNA complement(<744535..>745281)
/gene="SAS5"
/locus_tag="YOR213C"
/product="Sas5p"
/transcript_id="NM_001183632.1"
/db_xref="GeneID:854388"
CDS complement(744535..745281)
/gene="SAS5"
/locus_tag="YOR213C"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:11731479]"
/experiment="EXISTENCE:direct assay:GO:0004402 histone
acetyltransferase activity [PMID:12626510]"
/experiment="EXISTENCE:direct assay:GO:0016407
acetyltransferase activity [PMID:11731479]"
/experiment="EXISTENCE:direct assay:GO:0031509
subtelomeric heterochromatin formation [PMID:11731479]"
/experiment="EXISTENCE:direct assay:GO:0033255 SAS
acetyltransferase complex [PMID:12626510]"
/experiment="EXISTENCE:physical interaction:GO:0033255 SAS
acetyltransferase complex [PMID:15788653]"
/note="Subunit of the SAS complex (Sas2p, Sas4p, Sas5p);
acetylates free histones and nucleosomes and regulates
transcriptional silencing; stimulates Sas2p HAT activity"
/codon_start=1
/product="Sas5p"
/protein_id="NP_014856.1"
/db_xref="GeneID:854388"
/db_xref="SGD:S000005739"
/translation="MDHSIEVTFRVKTQQVIIPEQNIRGNELPLRRWQMELLMLDATG
KEVEPTILSKCIYHLHSSFKQPKRRLNSLPFFIKETGWGEFNLKIECFFIGNAGKFSI
EHDLTFEDDAYAVDYTVDVPHEFSHLNSELSKYFDLPWKVVSPEEEMSLRIADLPWIK
SLALIDEDMMTDVVQMILNDPAVQRAIENHPRREQFFMFITQLPDDLLMKIQAFLKLP
NKNSTKQERTNFGSDAIHKDEPVKAHNKLK"
gene complement(<745593..>746303)
/gene="SPR2"
/locus_tag="YOR214C"
/db_xref="GeneID:854389"
mRNA complement(<745593..>746303)
/gene="SPR2"
/locus_tag="YOR214C"
/product="Spr2p"
/transcript_id="NM_001183633.3"
/db_xref="GeneID:854389"
CDS complement(745593..746303)
/gene="SPR2"
/locus_tag="YOR214C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:10383953]"
/note="Putative spore wall protein; expression increases
during sporulation; not an essential gene; YOR214C has a
paralog, SPO19, that arose from the whole genome
duplication"
/codon_start=1
/product="Spr2p"
/protein_id="NP_014857.3"
/db_xref="GeneID:854389"
/db_xref="SGD:S000005740"
/translation="MLGLYLSSLFFAFFMAQVFATKYSITFTSDEYEEDETGQNEPGP
LVFHLDKNSLPPALLNQMEFNPYLVLADLPEEPRAVDSQEHTDTVLASKSVIDFLLED
PLTIVEHKKFSQIESILHEIMEDSIQKKVGADEVFEEIPKPKIYAYEDILVTNMSIIN
NSEMPTSTATLTSTISYLSSTTSLALSTGVTSVEIFPTITPGNITTIGGYENSSSSLM
PSMGILSFLFGLYLLLHP"
gene complement(<746725..>747282)
/gene="AIM41"
/locus_tag="YOR215C"
/db_xref="GeneID:854390"
mRNA complement(<746725..>747282)
/gene="AIM41"
/locus_tag="YOR215C"
/product="Aim41p"
/transcript_id="NM_001183634.1"
/db_xref="GeneID:854390"
CDS complement(746725..747282)
/gene="AIM41"
/locus_tag="YOR215C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:24769239|PMID:14562095|PMID:16823961|PMID:14576278]"
/note="hypothetical protein; the authentic protein is
detected in highly purified mitochondria in
high-throughput studies; null mutant displays reduced
frequency of mitochondrial genome loss"
/codon_start=1
/product="Aim41p"
/protein_id="NP_014858.1"
/db_xref="GeneID:854390"
/db_xref="SGD:S000005741"
/translation="MFRQSIRPLVSNRLTFIRYNSSPAYTAAVSLLKGDLKKAMIAKD
EMKKTAIRNMLSAIKNKEIALKGKSADEYSLYDMYSKLISQRKDSINEFLANKRDDLV
AKEQGEMDIIKKYMDQLPVSSELDIDQNVKKLLDALKTKAGEKKVQIKEIMGEIDWKS
LPTEWKTSPTAIKNSIVKQFKEIFK"
gene complement(<747525..>748979)
/gene="RUD3"
/locus_tag="YOR216C"
/gene_synonym="GRP1"
/db_xref="GeneID:854391"
mRNA complement(<747525..>748979)
/gene="RUD3"
/locus_tag="YOR216C"
/gene_synonym="GRP1"
/product="Rud3p"
/transcript_id="NM_001183635.3"
/db_xref="GeneID:854391"
CDS complement(747525..748979)
/gene="RUD3"
/locus_tag="YOR216C"
/gene_synonym="GRP1"
/experiment="EXISTENCE:direct assay:GO:0000139 Golgi
membrane [PMID:15504911]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0051020 GTPase
binding [PMID:15504911]"
/experiment="EXISTENCE:genetic interaction:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:10512869|PMID:10562277]"
/note="Golgi matrix protein; involved in the structural
organization of the cis-Golgi; interacts genetically with
COG3 and USO1"
/codon_start=1
/product="Rud3p"
/protein_id="NP_014859.3"
/db_xref="GeneID:854391"
/db_xref="SGD:S000005742"
/translation="MGKNKKKTGKKAKSHPHVEDVDETVNKPEEIINSVNVTVPPKMS
TDPEADGIVASPDDEGKDLSEGVDKQKVNDGLTVDTINPLEDKKAGDEMKELREEIER
LKLELSHKKDQETPNEDFKNELANVIKERDEFKTQYDTLLSKISSMKSIFNKMKEAQK
QLEEVQEQLTEYESQNLKLKKKLEATKTENSELQSTIVTLNTELENLEKEQESTEEVF
LEYESRIEALEDEKHDIIEKHSKELNTYRKEKDQLNLQVQELMIILENNKQDISDLRT
ERDELRQALESHEKEKAVLKNSLNDLELKIEEVDNKREEEARERDQEVKSLRSQLDTE
IETHNNDTEALESMKKQLEAMKEDASMKEKYEEESKQHILQIGKLRHEAIILNEHLTK
ALAMLKKSSDSESVDKELISNLLISFVSIPRADPRKFEVLELLSNFLNWDEDKKQQAG
LISNNESKNSSAVSRTESFVSLWTNYLEKESEKD"
gene <749301..>751886
/gene="RFC1"
/locus_tag="YOR217W"
/gene_synonym="CDC44"
/db_xref="GeneID:854392"
mRNA <749301..>751886
/gene="RFC1"
/locus_tag="YOR217W"
/gene_synonym="CDC44"
/product="replication factor C subunit 1"
/transcript_id="NM_001183636.1"
/db_xref="GeneID:854392"
CDS 749301..751886
/gene="RFC1"
/locus_tag="YOR217W"
/gene_synonym="CDC44"
/experiment="EXISTENCE:direct assay:GO:0003689 DNA clamp
loader activity [PMID:8995429]"
/experiment="EXISTENCE:direct assay:GO:0005663 DNA
replication factor C complex [PMID:7651383]"
/experiment="EXISTENCE:direct assay:GO:0006272 leading
strand elongation [PMID:1346062]"
/experiment="EXISTENCE:genetic interaction:GO:0000278
mitotic cell cycle [PMID:7516465]"
/experiment="EXISTENCE:genetic interaction:GO:0006298
mismatch repair [PMID:9504910]"
/experiment="EXISTENCE:mutant phenotype:GO:0000278 mitotic
cell cycle [PMID:6749598|PMID:6749599]"
/experiment="EXISTENCE:mutant phenotype:GO:0006281 DNA
repair [PMID:8770585]"
/note="Subunit of heteropentameric Replication factor C
(RF-C); RF-C is a DNA binding protein and ATPase that acts
as a clamp loader of the proliferating cell nuclear
antigen (PCNA) processivity factor for DNA polymerases
delta and epsilon"
/codon_start=1
/product="replication factor C subunit 1"
/protein_id="NP_014860.1"
/db_xref="GeneID:854392"
/db_xref="SGD:S000005743"
/translation="MVNISDFFGKNKKSVRSSTSRPTRQVGSSKPEVIDLDTESDQES
TNKTPKKMPVSNVIDVSETPEGEKKLPLPAKRKASSPTVKPASSKKTKPSSKSSDSAS
NITAQDVLDKIPSLDLSNVHVKENAKFDFKSANSNADPDEIVSEIGSFPEGKPNCLLG
LTIVFTGVLPTLERGASEALAKRYGARVTKSISSKTSVVVLGDEAGPKKLEKIKQLKI
KAIDEEGFKQLIAGMPAEGGDGEAAEKARRKLEEQHNIATKEAELLVKKEEERSKKLA
ATRVSGGHLERDNVVREEDKLWTVKYAPTNLQQVCGNKGSVMKLKNWLANWENSKKNS
FKHAGKDGSGVFRAAMLYGPPGIGKTTAAHLVAQELGYDILEQNASDVRSKTLLNAGV
KNALDNMSVVGYFKHNEEAQNLNGKHFVIIMDEVDGMSGGDRGGVGQLAQFCRKTSTP
LILICNERNLPKMRPFDRVCLDIQFRRPDANSIKSRLMTIAIREKFKLDPNVIDRLIQ
TTRGDIRQVINLLSTISTTTKTINHENINEISKAWEKNIALKPFDIAHKMLDGQIYSD
IGSRNFTLNDKIALYFDDFDFTPLMIQENYLSTRPSVLKPGQSHLEAVAEAANCISLG
DIVEKKIRSSEQLWSLLPLHAVLSSVYPASKVAGHMAGRINFTAWLGQNSKSAKYYRL
LQEIHYHTRLGTSTDKIGLRLDYLPTFRKRLLDPFLKQGADAISSVIEVMDDYYLTKE
DWDSIMEFFVGPDVTTAIIKKIPATVKSGFTRKYNSMTHPVAIYRTGSTIGGGGVGTS
TSTPDFEDVVDADDNPVPADDEETQDSSTDLKKDKLIKQKAKPTKRKTATSKPGGSKK
RKTKA"
gene complement(<752214..>755009)
/gene="STE13"
/locus_tag="YOR219C"
/gene_synonym="YCI1"
/db_xref="GeneID:854394"
mRNA complement(<752214..>755009)
/gene="STE13"
/locus_tag="YOR219C"
/gene_synonym="YCI1"
/product="Ste13p"
/transcript_id="NM_001183638.3"
/db_xref="GeneID:854394"
CDS complement(752214..755009)
/gene="STE13"
/locus_tag="YOR219C"
/gene_synonym="YCI1"
/experiment="EXISTENCE:direct assay:GO:0004177
aminopeptidase activity [PMID:6339075]"
/experiment="EXISTENCE:direct assay:GO:0005802 trans-Golgi
network [PMID:8308064]"
/experiment="EXISTENCE:direct assay:GO:0007323 peptide
pheromone maturation [PMID:6339075]"
/experiment="EXISTENCE:mutant phenotype:GO:0007323 peptide
pheromone maturation [PMID:6339075]"
/note="Dipeptidyl aminopeptidase; Golgi integral membrane
protein that cleaves on the carboxyl side of repeating
-X-Ala- sequences, required for maturation of alpha
factor, transcription is induced by a-factor"
/codon_start=1
/product="Ste13p"
/protein_id="NP_014862.3"
/db_xref="GeneID:854394"
/db_xref="SGD:S000005745"
/translation="MSASTHSHKRKNSHLFPQRKSSNSSMDKPFFPNNDSVANTDPQS
NENGHTINEIRPTEATIDVTDVPQTPFLQEQYSMRPRRESFQFNDIENQHHTHSFFSV
NKFNRRWGEWSLPEKRSYVLVFTLIALSVLVLLVILIPSKLLPTKITRPKTSAGDSSL
GKRSFSIENVLNGDFAIPEDTFHFIDPPQRLLGQDSDPGLYFTTKEIDGHTNFIAKQL
FDETFEVNLGGNRFLYEGVEFTVSTVQINYKLDKLIFGTNLESEFRHSSKGFYWIKDL
NTGNIEPILPPEKSDDNYELGLSKLSYAHFSPAYNYIYFVYENNLFLQQVNSGVAKKV
TEDGSKDIFNAKPDWIYEEEVLASDQAIWWAPDDSKAVFARFNDTSVDDIRLNRYTNM
NEAYLSDTKIKYPKPGFQNPQFDLFLVNLQNGIIYSINTGGQKDSILYNGKWISPDTF
RFEITDRNSKILDVKVYDIPSSQMLTVRNTNSNLFNGWIEKTKDILSIPPKPELKRMD
YGYIDIHADSRGFSHLFYYPTVFAKEPIQLTKGNWEVTGNGIVGYEYETDTIFFTANE
IGVMSQHLYSISLTDSTTQNTFQSLQNPSDKYDFYDFELSSSARYAISKKLGPDTPIK
VAGPLTRVLNVAEIHDDSILQLTKDEKFKEKIKNYDLPITSYKTMVLDDGVEINYIEI
KPANLNPKKKYPILVNIYGGPGSQTFTTKSSLAFEQAVVSGLDVIVLQIEPRGTGGKG
WSFRSWAREKLGYWEPRDITEVTKKFIQRNSQHIDESKIAIWGWSYGGFTSLKTVELD
NGDTFKYAMAVAPVTNWTLYDSVYTERYMNQPSENHEGYFEVSTIQNFKSFESLKRLF
IVHGTFDDNVHIQNTFRLVDQLNLLGLTNYDMHIFPDSDHSIRYHNAQRIVFQKLYYW
LRDAFAERFDNTEVLHL"
gene <755328..>756125
/gene="RCN2"
/locus_tag="YOR220W"
/db_xref="GeneID:854395"
mRNA <755328..>756125
/gene="RCN2"
/locus_tag="YOR220W"
/product="Rcn2p"
/transcript_id="NM_001183639.1"
/db_xref="GeneID:854395"
CDS 755328..756125
/gene="RCN2"
/locus_tag="YOR220W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to the cytoplasm;
phosphorylated in response to alpha factor; protein
abundance increases in response to DNA replication stress"
/codon_start=1
/product="Rcn2p"
/protein_id="NP_014863.1"
/db_xref="GeneID:854395"
/db_xref="SGD:S000005746"
/translation="MANQKQMRTQILITDIPSGKFTSKWPTQLEKTLFKEQFPNLQSH
LQYYTPLPFLNRIIIIFDNEDDTLQVFKFLQELLAKENSGPMKLFVTESLLNNQHPRS
RSTDDAVSLQDNNLALLEDHRNKPLLSINTDPGVTGVDSSSLNKGGSSLSPDKSSLES
PTMLKLSTDSKPFSYQEPLPKLSRSSSSTSNLSLNRSSQTSLPSQLENKDKSASGTKC
LFASKPLGLTIDTSTRSNAASCTENDVNATASNPPKSPSITVNEFFH"
gene complement(<756476..>757558)
/gene="MCT1"
/locus_tag="YOR221C"
/db_xref="GeneID:854396"
mRNA complement(<756476..>757558)
/gene="MCT1"
/locus_tag="YOR221C"
/product="[acyl-carrier-protein] S-malonyltransferase"
/transcript_id="NM_001183640.3"
/db_xref="GeneID:854396"
CDS complement(756476..757558)
/gene="MCT1"
/locus_tag="YOR221C"
/EC_number="2.3.1.39"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:mutant phenotype:GO:0004314
[acyl-carrier-protein] S-malonyltransferase activity
[PMID:9388293]"
/experiment="EXISTENCE:mutant phenotype:GO:0005739
mitochondrion [PMID:9388293]"
/experiment="EXISTENCE:mutant phenotype:GO:0006631 fatty
acid metabolic process [PMID:9388293]"
/note="Predicted malonyl-CoA:ACP transferase; putative
component of a type-II mitochondrial fatty acid synthase
that produces intermediates for phospholipid remodeling"
/codon_start=1
/product="[acyl-carrier-protein] S-malonyltransferase"
/protein_id="NP_014864.4"
/db_xref="GeneID:854396"
/db_xref="SGD:S000005747"
/translation="MKLLTFPGQGTSISISILKAIIRNKSREFQTILSQNGKESNDLL
QYIFQNPSSPGSIAVCSNLFYQLYQILSNPSDPQDQAPKNMTKIDSPDKKDNEQCYLL
GHSLGELTCLSVNSLFSLKDLFDIANFRNKLMVTSTEKYLVAHNINRSNKFEMWALSS
PRATDLPQEVQKLLNSPNLLSSSQNTISVANANSVKQCVVTGLVDDLESLRTELNLRF
PRLRITELTNPYNIPFHNSTVLRPVQEPLYDYIWDILKKNGTHTLMELNHPIIANLDG
NISYYIHHALDRFVKCSSRTVQFTMCYDTINSGTPVEIDKSICFGPGNVIYNLIRRNC
PQVDTIEYTSLATIDAYHKAAEENKD"
gene <758330..>759253
/gene="ODC2"
/locus_tag="YOR222W"
/db_xref="GeneID:854397"
mRNA <758330..>759253
/gene="ODC2"
/locus_tag="YOR222W"
/product="mitochondrial 2-oxodicarboxylate carrier"
/transcript_id="NM_001183641.1"
/db_xref="GeneID:854397"
CDS 758330..759253
/gene="ODC2"
/locus_tag="YOR222W"
/experiment="EXISTENCE:direct assay:GO:0005310
dicarboxylic acid transmembrane transporter activity
[PMID:11013234]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:11013234]"
/experiment="EXISTENCE:direct assay:GO:0006839
mitochondrial transport [PMID:11013234]"
/note="Mitochondrial inner membrane transporter;
2-oxodicarboxylate transporter, exports 2-oxoadipate and
2-oxoglutarate from the mitochondrial matrix to the
cytosol for use in lysine and glutamate biosynthesis and
in lysine catabolism; ODC2 has a paralog, ODC1, that arose
from the whole genome duplication"
/codon_start=1
/product="mitochondrial 2-oxodicarboxylate carrier"
/protein_id="NP_014865.1"
/db_xref="GeneID:854397"
/db_xref="SGD:S000005748"
/translation="MSSDSNAKPLPFIYQFISGAVAGISELTVMYPLDVVKTRFQLEV
TTPTAAAVGKQVERYNGVIDCLKKIVKKEGFSRLYRGISSPMLMEAPKRATKFACNDQ
YQKIFKNLFNTNETTQKISIAAGASAGMTEAAVIVPFELIKIRMQDVKSSYLGPMDCL
KKTIKNEGIMGLYKGIESTMWRNALWNGGYFGVIYQVRNSMPVAKTKGQKTRNDLIAG
AIGGTVGTMLNTPFDVVKSRIQSVDAVSSAVKKYNWCLPSLLVIYREEGFRALYKGFV
PKVCRLAPGGSLMLVVFTGMMNFFRDLKYGH"
gene complement(759326..759529)
/gene="SNR35"
/locus_tag="YNCO0024C"
/db_xref="GeneID:9164977"
ncRNA complement(759326..759529)
/ncRNA_class="snoRNA"
/gene="SNR35"
/locus_tag="YNCO0024C"
/product="SNR35"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:7630735]"
/experiment="EXISTENCE:physical interaction:GO:0031429 box
H/ACA snoRNP complex [PMID:15923376]"
/note="H/ACA box small nucleolar RNA (snoRNA); predicted
to guide pseudouridylation of small subunit (SSU) rRNA at
position 1191"
/transcript_id="NR_132261.1"
/db_xref="GeneID:9164977"
/db_xref="SGD:S000007299"
gene <759782..>760660
/gene="DSC3"
/locus_tag="YOR223W"
/db_xref="GeneID:854398"
mRNA <759782..>760660
/gene="DSC3"
/locus_tag="YOR223W"
/product="Dsc3p"
/transcript_id="NM_001183642.3"
/db_xref="GeneID:854398"
CDS 759782..760660
/gene="DSC3"
/locus_tag="YOR223W"
/experiment="EXISTENCE:direct assay:GO:0000328 fungal-type
vacuole lumen [PMID:21777356]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:21777356]"
/experiment="EXISTENCE:direct assay:GO:0044695 Dsc E3
ubiquitin ligase complex [PMID:22681890]"
/experiment="EXISTENCE:genetic interaction:GO:0140624 EGAD
pathway [PMID:31368600]"
/experiment="EXISTENCE:mutant phenotype:GO:0140624 EGAD
pathway [PMID:31368600]"
/note="Subunit of the DSC ubiquitin ligase complex;
involved in the endosome and Golgi-associated degradation
pathway (EGAD), contributing to proteostasis and lipid
homeostasis; localizes to the ER and vacuole lumen;
overexpression affects endocytic protein trafficking;
ortholog of fission yeast dsc3"
/codon_start=1
/product="Dsc3p"
/protein_id="NP_014866.3"
/db_xref="GeneID:854398"
/db_xref="SGD:S000005749"
/translation="MSAEPLLPTHNGSQGGEVRSPDQKFIVIRFSDVSVRDLQLNISN
VPFSNINTHWLRRMCRELRPQQTQKRRLKFIRNGSILNTHSKIAEELTHYFDTANNSN
VATGTSVAPEQNNYYIHCIIGTEELTQAELANEDLKDDATPSNDSMTTQAIGFDRLRS
VGFTEQEIELLRQQFRATYGDLEEEEERLAQNGNRDDEGHDIRQLEEQWMESGSGTAQ
GNGAGGGNEDRFNSVPIANIKHNKDLLLGICVGFFFGVFGILLMKFDGLFNRRQKMAI
FAGVIVNVMFCLVRGF"
gene complement(<760825..>761265)
/gene="RPB8"
/locus_tag="YOR224C"
/db_xref="GeneID:854399"
mRNA complement(<760825..>761265)
/gene="RPB8"
/locus_tag="YOR224C"
/product="DNA-directed RNA polymerase core subunit RPB8"
/transcript_id="NM_001183643.1"
/db_xref="GeneID:854399"
CDS complement(760825..761265)
/gene="RPB8"
/locus_tag="YOR224C"
/experiment="EXISTENCE:direct assay:GO:0003899
DNA-directed RNA polymerase activity
[PMID:3905793|PMID:9837969|PMID:8288647]"
/experiment="EXISTENCE:direct assay:GO:0003968
RNA-directed RNA polymerase activity [PMID:18004386]"
/experiment="EXISTENCE:direct assay:GO:0005665 RNA
polymerase II, core complex
[PMID:2183013|PMID:2186966|PMID:1331084]"
/experiment="EXISTENCE:direct assay:GO:0005666 RNA
polymerase III complex [PMID:10611227|PMID:3905793]"
/experiment="EXISTENCE:direct assay:GO:0005736 RNA
polymerase I complex [PMID:11486042|PMID:9837969]"
/experiment="EXISTENCE:direct assay:GO:0006360
transcription by RNA polymerase I [PMID:9837969]"
/experiment="EXISTENCE:direct assay:GO:0042797 tRNA
transcription by RNA polymerase III [PMID:3905793]"
/experiment="EXISTENCE:genetic interaction:GO:0006383
transcription by RNA polymerase III [PMID:9927738]"
/experiment="EXISTENCE:physical interaction:GO:0006360
transcription by RNA polymerase I [PMID:11486042]"
/experiment="EXISTENCE:physical interaction:GO:0006366
transcription by RNA polymerase II [PMID:11486042]"
/experiment="EXISTENCE:physical interaction:GO:0006383
transcription by RNA polymerase III [PMID:11486042]"
/note="RNA polymerase subunit ABC14.5; common to RNA
polymerases I, II, and III"
/codon_start=1
/product="DNA-directed RNA polymerase core subunit RPB8"
/protein_id="NP_014867.1"
/db_xref="GeneID:854399"
/db_xref="SGD:S000005750"
/translation="MSNTLFDDIFQVSEVDPGRYNKVCRIEAASTTQDQCKLTLDINV
ELFPVAAQDSLTVTIASSLNLEDTPANDSSATRSWRPPQAGDRSLADDYDYVMYGTAY
KFEEVSKDLIAVYYSFGGLLMRLEGNYRNLNNLKQENAYLLIRR"
gene complement(<761614..>762084)
/gene="ISU2"
/locus_tag="YOR226C"
/gene_synonym="NUA2"
/db_xref="GeneID:854401"
mRNA complement(<761614..>762084)
/gene="ISU2"
/locus_tag="YOR226C"
/gene_synonym="NUA2"
/product="putative iron-binding protein ISU2"
/transcript_id="NM_001183645.3"
/db_xref="GeneID:854401"
CDS complement(761614..762084)
/gene="ISU2"
/locus_tag="YOR226C"
/gene_synonym="NUA2"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:15143178|PMID:10468587]"
/experiment="EXISTENCE:genetic interaction:GO:0002098 tRNA
wobble uridine modification [PMID:17283054]"
/experiment="EXISTENCE:mutant phenotype:GO:0006879
intracellular iron ion homeostasis [PMID:10468587]"
/experiment="EXISTENCE:mutant phenotype:GO:0016226
iron-sulfur cluster assembly
[PMID:15143178|PMID:10588895]"
/note="Mitochondrial protein required for iron-sulfur
protein synthesis; performs scaffolding function during
Fe/S cluster assembly; involved in Fe-S cluster assembly
for both mitochondrial and cytosolic proteins; protein
abundance increases under DNA replication stress; ISU2 has
a paralog, ISU1, that arose from the whole genome
duplication; isu1 isu2 double mutant is inviable; human
homolog ISCU implicated in mitochondrial myopathy, can
complement isu1 isu2 double mutant"
/codon_start=1
/product="putative iron-binding protein ISU2"
/protein_id="NP_014869.3"
/db_xref="GeneID:854401"
/db_xref="SGD:S000005752"
/translation="MFARLANPAHFKPLTGSHITRAAKRLYHPKVIDHYTNPRNVGSM
DKSLANVGTGIVGAPACGDVIKLQIQVNDKSGIIENVKFKTFGCGSAIASSSYMTELV
RGMSLDEAVKIKNTEIAKELSLPPVKLHCSMLAEDAIKAAIKDYKTKRNPSVLH"
gene <762825..>766565
/gene="HER1"
/locus_tag="YOR227W"
/db_xref="GeneID:854402"
mRNA <762825..>766565
/gene="HER1"
/locus_tag="YOR227W"
/product="Her1p"
/transcript_id="NM_001183646.1"
/db_xref="GeneID:854402"
CDS 762825..766565
/gene="HER1"
/locus_tag="YOR227W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:genetic interaction:GO:0007029
endoplasmic reticulum organization [PMID:18667535]"
/note="hypothetical protein; required for proliferation or
remodeling of the ER that is caused by overexpression of
Hmg2p; may interact with ribosomes, based on
co-purification experiments; HER1 has a paralog, GIP3,
that arose from the whole genome duplication"
/codon_start=1
/product="Her1p"
/protein_id="NP_014870.1"
/db_xref="GeneID:854402"
/db_xref="SGD:S000005753"
/translation="MSSKLKYTDIDVPLDWLYKGKRRNRTKSAASTRTSEATTTSVKK
TATLPSTAAVPTKTIASPQRPLSGQNVNNELSNSKPAVSAEKVSQQGQVPTRRTRSHS
VSYGLLQKKNNNDDTTDSPKISRIRTAQDQPVKETKSSTLAEPIVSKKGRSRSSSIST
SLNERSKKSLFGSLFGRRPSTTPSHVVERPLSSQNDHKKSTELPPIDTRQSKISTPTS
TPTTASSKPSSSGGNRHSDGSLTSKLLSIPHNILETSSTNFNAHHHIQSHHSSGREQD
SPHSESSDLPPILEKETTQKQLQKVSKVNLKRVTIAVQEFNSDPPQQLPSRKPKRGNV
LIPEDMISAPPLISLGITNSSDQSSFQSNISPSYSKDSKEYKLALENFKKAAKEAEKH
QKDAYYVAERMAQEVANYKARQLKTSPLTGATNSAADSATDQESSSLDARASKLHIDK
PINVGAHPFETHQDDNIKYSSHLEQTLDVAYTRCCHLREILPIPSTLRQVKGKTAPLQ
TLKFLNPKPTLVDILSFCDFIAITPIHNIIFDNVSLTHDMFKIVICSLVTSPVVEKLG
LRNVVINEQSWKLLCKFLLQNKTLIKLDISQTKARTDLNDSNYRDQMDWELFCEVLRN
REGRPLEELLLNGLRFDKMSFSHFKNILLTFAQMNPKNPIRLGMANVEFSTECFDFLF
NWMSEYNVQGVDLAYNNLESLAKRMIKKLARLPYKHLEYFTLNSTNITSVDDMSYILK
YLSRLPSIKFLDLSNLPQLFPGILTSGYKYFPQFPQLKRIHFDFDDLSIKETTMLVSI
LAKCETLSHVSLIGQSPMPDASKISDSTDEPDKSKDEKKEQIVFMRNTLWASLYAFVR
DSHNLVSLDVDYDQVPDEIQSRIALCLMHNMKRIMDSSFKLDELTVQDDLIFDGSLIT
ETAEEVLKRLNDKSLLQNDVGKKYLLKKYFEKMEKVHHNVQNTIDSMFEKRKSGELPL
QEKENLLRLLLLEKNLSNILDIFASMPNIADVVPFSKADNSFPNIGDSTVSANYNDGI
RPSLKHLDSDRLINDVSIPENDSSIRPHLMATDSGRIIDVTTGKALLFKSSSNTSLAG
KRQEEEEGELHKWGVFVQHQSSRHNSGLPSSANSSRISGSLTPDSSVAGGKKGESSRT
SGTRPKILPKIPTGAELRDAIIKAKGIDSVDDLIKNVTSEKVGLESLYGDELNSRSPS
NDSLQESQQKAPLQRPLVEDETVTKKYDKLLNDLSNVRHSKT"
rep_origin 766605..766788
/note="ARS1523; Autonomously Replicating Sequence"
/db_xref="SGD:S000118492"
gene complement(<766869..>767777)
/gene="MCP1"
/locus_tag="YOR228C"
/db_xref="GeneID:854403"
mRNA complement(<766869..>767777)
/gene="MCP1"
/locus_tag="YOR228C"
/product="Mcp1p"
/transcript_id="NM_001183647.3"
/db_xref="GeneID:854403"
CDS complement(766869..767777)
/gene="MCP1"
/locus_tag="YOR228C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane
[PMID:23781023|PMID:16407407]"
/experiment="EXISTENCE:direct assay:GO:0031966
mitochondrial membrane [PMID:23781023]"
/experiment="EXISTENCE:genetic interaction:GO:0007005
mitochondrion organization [PMID:23781023]"
/experiment="EXISTENCE:genetic interaction:GO:0055088
lipid homeostasis [PMID:23781023]"
/note="Mitochondrial hypothetical protein involved in
lipid homeostasis; integral membrane protein that
localizes to the mitochondrial outer membrane; involved in
mitochondrial morphology; interacts genetically with
MDM10, and other members of the ERMES complex; contains
five predicted transmembrane domains"
/codon_start=1
/product="Mcp1p"
/protein_id="NP_014871.3"
/db_xref="GeneID:854403"
/db_xref="SGD:S000005754"
/translation="MIKLHEVPPEPVDPASLPHDVNAHSPEGDGNPDKRKKIFGIPYP
FSRSSCRRFLWNCQKISVLPMALYFPLHAANTLITPAVSPDSAPDDVLMMVREILPSI
TTKLLVAGITLHVSAGVLLRIVNNWNKPRRNRHRHLKISAEQDLSQDSIGLTGGISGY
LFGLYKTFRIPPQVISGYILVPVLIYHLLIMKWVPNSISTEVDFASIKQLLSSKNRWW
KWLGGLVPLAILLESGVYHIGSGLCRYFGVRKMTSRKKWSTAINLLTLVGFVSLIRLM
KEDSTKLGPNQFESIFKKIRLLLHVN"
misc_feature 768106..768128
/note="ETC7; Chromosome-organizing-clamp; tethers
chromosomal regions to the nuclear periphery; binds TFIIIC
transcription factor but does not recruit RNA Polymerase
III; can act as a transcription-blocking insulator or as a
heterochromatin barrier element; located between
divergently transcribed genes MCP1 and WTM2"
/db_xref="SGD:S000077078"
gene <768409..>769812
/gene="WTM2"
/locus_tag="YOR229W"
/db_xref="GeneID:854404"
mRNA <768409..>769812
/gene="WTM2"
/locus_tag="YOR229W"
/product="transcriptional modulator"
/transcript_id="NM_001183648.1"
/db_xref="GeneID:854404"
CDS 768409..769812
/gene="WTM2"
/locus_tag="YOR229W"
/experiment="EXISTENCE:direct assay:GO:0000981 DNA-binding
transcription factor activity, RNA polymerase II-specific
[PMID:16980392]"
/experiment="EXISTENCE:direct assay:GO:0003714
transcription corepressor activity [PMID:9234739]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476|PMID:9234739]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0045944 positive
regulation of transcription by RNA polymerase II
[PMID:16980392]"
/experiment="EXISTENCE:genetic interaction:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:9234739]"
/experiment="EXISTENCE:genetic interaction:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:16980392]"
/experiment="EXISTENCE:genetic interaction:GO:0051321
meiotic cell cycle [PMID:9234739]"
/experiment="EXISTENCE:genetic interaction:GO:0061186
negative regulation of silent mating-type cassette
heterochromatin formation [PMID:9234739]"
/experiment="EXISTENCE:mutant phenotype:GO:0000981
DNA-binding transcription factor activity, RNA polymerase
II-specific [PMID:16980392]"
/experiment="EXISTENCE:mutant phenotype:GO:0006974 DNA
damage response [PMID:16980392]"
/experiment="EXISTENCE:mutant phenotype:GO:0031503
protein-containing complex localization [PMID:16452505]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:16980392]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:16452505]"
/note="Transcriptional modulator; involved in regulation
of meiosis, silencing, and expression of RNR genes;
involved in response to replication stress; contains WD
repeats; relocalizes to the cytosol in response to
hypoxia; WTM2 has a paralog, UME1, that arose from the
whole genome duplication"
/codon_start=1
/product="transcriptional modulator"
/protein_id="NP_014872.1"
/db_xref="GeneID:854404"
/db_xref="SGD:S000005755"
/translation="MAKSKSSQGASGARRKPAPSLYQHISSFKPQFSTRVDDVLHFSK
TLTWRSEIIPDKSKGTLTTSLLYSQGSDIYEIDTTLPLKTFYDDDDDDDNDDDDEEGN
GKTKSAATPNPEYGDAFQDVEGKPLRPKWIYQGETVAKMQYLESSDDSTAIAMSKNGS
LAWFRDEIKVPVHIVQEMMGPATRYSSIHSLTRPGSLAVSDFDVSTNMDTVVKSQSNG
YEEDSILKIIDNSDRPGDILRTVHVPGTNVAHSVRFFNNHLFASCSDDNILRFWDTRT
ADKPLWTLSEPKNGRLTSFDSSQVTENLFVTGFSTGVIKLWDARAVQLATTDLTHRQN
GEEPIQNEIAKLFHSGGDSVVDILFSQTSATEFVTVGGTGNVYHWDMEYSFSRNDDDN
EDEVRVAAPEELQGQCLKFFHTGGTRRSSNQFGKRNTVALHPVINDFVGTVDSDSLVT
AYKPFLASDFIGRGYDD"
gene <770800..>772113
/gene="WTM1"
/locus_tag="YOR230W"
/db_xref="GeneID:854405"
mRNA <770800..>772113
/gene="WTM1"
/locus_tag="YOR230W"
/product="transcriptional modulator"
/transcript_id="NM_001183649.3"
/db_xref="GeneID:854405"
CDS 770800..772113
/gene="WTM1"
/locus_tag="YOR230W"
/experiment="EXISTENCE:direct assay:GO:0003714
transcription corepressor activity [PMID:9234739]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11914276|PMID:9234739]"
/experiment="EXISTENCE:direct assay:GO:0045944 positive
regulation of transcription by RNA polymerase II
[PMID:16980392]"
/experiment="EXISTENCE:genetic interaction:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:9234739]"
/experiment="EXISTENCE:genetic interaction:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:16980392]"
/experiment="EXISTENCE:genetic interaction:GO:0051321
meiotic cell cycle [PMID:9234739]"
/experiment="EXISTENCE:genetic interaction:GO:0061186
negative regulation of silent mating-type cassette
heterochromatin formation [PMID:9234739]"
/experiment="EXISTENCE:mutant phenotype:GO:0006606 protein
import into nucleus [PMID:16432237]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:16980392]"
/experiment="EXISTENCE:mutant phenotype:GO:0051457
maintenance of protein location in nucleus
[PMID:16452505]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:16452505]"
/experiment="EXISTENCE:physical interaction:GO:0006606
protein import into nucleus [PMID:16432237]"
/note="Transcriptional modulator; involved in regulation
of meiosis, silencing, and expression of RNR genes; acts
as a nuclear anchor to retain the ribonucleotide reductase
small subunit heterodimer, Rnr2p-Rnr4p, in the nucleus
when cells are not in S phase; contains WD repeats"
/codon_start=1
/product="transcriptional modulator"
/protein_id="NP_014873.3"
/db_xref="GeneID:854405"
/db_xref="SGD:S000005756"
/translation="MPKKVWKSSTPSTYEHISSLRPKFVSRVDNVLHQRKSLTFSNVV
VPDKKNNTLTSSVIYSQGSDIYEIDFAVPLQEAASEPVKDYGDAFEGIENTSLSPKFV
YQGETVSKMAYLDKTGETTLLSMSKNGSLAWFKEGIKVPIHIVQELMGPATSYASIHS
LTRPGDLPEKDFSLAISDFGISNDTETIVKSQSNGDEEDSILKIIDNAGKPGEILRTV
HVPGTTVTHTVRFFDNHIFASCSDDNILRFWDTRTSDKPIWVLGEPKNGKLTSFDCSQ
VSNNLFVTGFSTGIIKLWDARAAEAATTDLTYRQNGEDPIQNEIANFYHAGGDSVVDV
QFSATSSSEFFTVGGTGNIYHWNTDYSLSKYNPDDTIAPPQDATEESQTKSLRFLHKG
GSRRSPKQIGRRNTAAWHPVIENLVGTVDDDSLVSIYKPYTEESE"
gene <772601..>774127
/gene="MKK1"
/locus_tag="YOR231W"
/gene_synonym="SSP32"
/db_xref="GeneID:854406"
mRNA <772601..>774127
/gene="MKK1"
/locus_tag="YOR231W"
/gene_synonym="SSP32"
/product="mitogen-activated protein kinase kinase MKK1"
/transcript_id="NM_001183650.1"
/db_xref="GeneID:854406"
CDS 772601..774127
/gene="MKK1"
/locus_tag="YOR231W"
/gene_synonym="SSP32"
/EC_number="2.7.12.2"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:12361575]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:23924898|PMID:12361575]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:12361575|PMID:23924898]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:12361575]"
/experiment="EXISTENCE:genetic interaction:GO:0000196 cell
integrity MAPK cascade [PMID:8386320]"
/experiment="EXISTENCE:genetic interaction:GO:0000425
pexophagy [PMID:20385774]"
/experiment="EXISTENCE:genetic interaction:GO:0060237
regulation of fungal-type cell wall organization
[PMID:8386320]"
/experiment="EXISTENCE:mutant phenotype:GO:0007165 signal
transduction [PMID:8386320]"
/note="MAPKK involved in the protein kinase C signaling
pathway; involved in control of cell integrity; upon
activation by Bck1p phosphorylates downstream target,
Slt2p; functionally redundant with Mkk2p; MKK1 has a
paralog, MKK2, that arose from the whole genome
duplication"
/codon_start=1
/product="mitogen-activated protein kinase kinase MKK1"
/protein_id="NP_014874.1"
/db_xref="GeneID:854406"
/db_xref="SGD:S000005757"
/translation="MASLFRPPESAKCNPNSPRLKLPLLRNNQVDENNIYLTSNGSST
TAYSSHTPEPLTSSTSTLFSQTRLHPSDSSMTLNTMKKRPAPPSLPSLSINSQSKCKT
LPELVPIADVSDGKHDLGLKQRVIAENELSGNSDLTPSSMASPFSHTNTSSPYLRNDL
SNSVGSDFSNLISAYEQSSSPIKSSSQPKSSSESYIDLNSVRDVDQLDENGWKYANLK
DRIETLGILGEGAGGSVSKCKLKNGSKIFALKVINTLNTDPEYQKQIFRELQFNRSFQ
SEYIVRYYGMFTDDENSSIYIAMEYMGGRSLDAIYKNLLERGGRISEKVLGKIAEAVL
RGLSYLHEKKVIHRDIKPQNILLNENGQVKLCDFGVSGEAVNSLATTFTGTSFYMAPE
RIQGQPYSVTSDVWSLGLTILEVANGKFPCSSEKMAANIAPFELLMWILTFTPELKDE
PESNIIWSPSFKSFIDYCLKKDSRERPSPRQMINHPWIKGQMKKNVNMEKFVRKCWKD
"
gene <774573..>775259
/gene="MGE1"
/locus_tag="YOR232W"
/gene_synonym="YGE1"
/db_xref="GeneID:854407"
mRNA <774573..>775259
/gene="MGE1"
/locus_tag="YOR232W"
/gene_synonym="YGE1"
/product="mitochondrial nucleotide exchange factor MGE1"
/transcript_id="NM_001183651.3"
/db_xref="GeneID:854407"
CDS 774573..775259
/gene="MGE1"
/locus_tag="YOR232W"
/gene_synonym="YGE1"
/experiment="EXISTENCE:direct assay:GO:0000774
adenyl-nucleotide exchange factor activity
[PMID:12756240|PMID:9048947]"
/experiment="EXISTENCE:direct assay:GO:0001405 PAM
complex, Tim23 associated import motor
[PMID:14638855|PMID:14517234]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:11914276|PMID:16823961|PMID:14576278|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:8022808]"
/experiment="EXISTENCE:direct assay:GO:0042026 protein
refolding [PMID:9973563]"
/experiment="EXISTENCE:mutant phenotype:GO:0000774
adenyl-nucleotide exchange factor activity [PMID:8918457]"
/experiment="EXISTENCE:mutant phenotype:GO:0030150 protein
import into mitochondrial matrix [PMID:8022808]"
/note="Mitochondrial matrix cochaperone; nucleotide
release factor for Ssc1p in protein translocation and
folding; also acts as cochaperone for Ssq1p in folding of
Fe-S cluster proteins; acts as oxidative sensor to
regulate mitochondrial Ssc1p; in presence of oxidative
stress, dimeric Mge1p becomes a monomer and unable to
regulate Ssc1p function; homolog of E. coli GrpE and human
Mge1 (GRPEL1), which also responds to oxidative stress"
/codon_start=1
/product="mitochondrial nucleotide exchange factor MGE1"
/protein_id="NP_014875.3"
/db_xref="GeneID:854407"
/db_xref="SGD:S000005758"
/translation="MRAFSAATVRATTRKSFIPMAPRTPFVTPSFTKNVGSMRRMRFY
SDEAKSEESKENNEDLTEEQSEIKKLESQLSAKTKEASELKDRLLRSVADFRNLQQVT
KKDIQKAKDFALQKFAKDLLESVDNFGHALNAFKEEDLQKSKEISDLYTGVRMTRDVF
ENTLRKHGIEKLDPLGEPFDPNKHEATFELPQPDKEPGTVFHVQQLGFTLNDRVIRPA
KVGIVKGEEN"
gene <775846..>778248
/gene="KIN4"
/locus_tag="YOR233W"
/gene_synonym="KIN3; KIN31"
/db_xref="GeneID:854408"
mRNA <775846..>778248
/gene="KIN4"
/locus_tag="YOR233W"
/gene_synonym="KIN3; KIN31"
/product="putative serine/threonine protein kinase KIN4"
/transcript_id="NM_001183652.1"
/db_xref="GeneID:854408"
CDS 775846..778248
/gene="KIN4"
/locus_tag="YOR233W"
/gene_synonym="KIN3; KIN31"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0004674 protein
serine/threonine kinase activity [PMID:17967947]"
/experiment="EXISTENCE:direct assay:GO:0005816 spindle
pole body [PMID:16039590]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:16039591]"
/experiment="EXISTENCE:direct assay:GO:0005938 cell cortex
[PMID:16039591]"
/experiment="EXISTENCE:genetic interaction:GO:0000011
vacuole inheritance [PMID:37509134]"
/experiment="EXISTENCE:genetic interaction:GO:0045033
peroxisome inheritance [PMID:37509134]"
/experiment="EXISTENCE:mutant phenotype:GO:0004674 protein
serine/threonine kinase activity [PMID:24850890]"
/experiment="EXISTENCE:mutant phenotype:GO:0031578 mitotic
spindle orientation checkpoint signaling
[PMID:16039590|PMID:16039591]"
/experiment="EXISTENCE:mutant phenotype:GO:0045033
peroxisome inheritance [PMID:37509134]"
/experiment="EXISTENCE:mutant phenotype:GO:0071988 protein
localization to spindle pole body [PMID:24850890]"
/note="Serine/threonine protein kinase; inhibits the
mitotic exit network (MEN) when the spindle position
checkpoint (SPOC) is activated; regulates peroxisomal
transport and vacuolar inheritance along with Frk1p;
protects the peroxisomal Myo2p receptor Inp2p from
degradation in mother cells along with Frk1p; localizes
asymmetrically to mother cell cortex, spindle pole body
and bud neck; KIN4 has a paralog, FRK1, that arose from
the whole genome duplication"
/codon_start=1
/product="putative serine/threonine protein kinase KIN4"
/protein_id="NP_014876.1"
/db_xref="GeneID:854408"
/db_xref="SGD:S000005759"
/translation="MASVPKRHTYGGNVVTDRDRHSLQRNNEILHPIHKNQRKHATFG
PYIIGSTLGEGEFGKVKLGWTKASSSNEVPKQVAIKLIRRDTIKKDADKEIKIYREIN
ALKHLTHPNIIYLEEVLQNSKYIGIVLEFVSGGEFYKYIQRKRRLKESSACRLFAQLI
SGVNYMHYKGLVHRDLKLENLLLDKHENLVITDFGFVNEFFEDNELMKTSCGSPCYAA
PELVVSTKAYEARKADVWSCGVILYAMLAGYLPWDDDHENPTGDDIARLYKYITQTPL
KFPEYITPIPRDLLRRILVPNPRRRINLQTIKRHVWLKPHEAFLSIQPNYWDEHLQKE
RPKPPNKGDVGRHSTYSSSASSYSKSRDRNSLIIESTLEQHRMSPQLATSRPASPTFS
TGSKVVLNDTKNDMKESNINGERTSASCRYTRDSKGNGQTQIEQVSARHSSRGNKHTS
VAGLVTIPGSPTTARTRNAPSSKLTEHVKDSSQTSFTQEEFHRIGNYHVPRSRPRPTS
YYPGLSRNTADNSLADIPVNKLGSNGRLTDAKDPVPLNAIHDTNKATISNNSIMLLSE
GPAAKTSPVDYHYAIGDLNHGDKPITEVIDKINKDLTHKAAENGFPRESIDPESTSTI
LVTKEPTNSTDEDHVESQLENVGHSSNKSDASSDKDSKKIYEKKRFSFMSLYSSLNGS
RSTVESRTSKGNAPPVSSRNPSGQSNRSNIKITQQQPRNLSDRVPNPDKKINDNRIRD
NAPSYAESENPGRSVRASVMVSTLREENRSELSNEGNNVEAQTSTARKVLNFFKRRSM
RV"
gene complement(<778555..>779405)
/gene="RPL33B"
/locus_tag="YOR234C"
/gene_synonym="RPL37B"
/db_xref="GeneID:854409"
mRNA complement(join(<778555..778859,779387..>779405))
/gene="RPL33B"
/locus_tag="YOR234C"
/gene_synonym="RPL37B"
/product="60S ribosomal protein eL33 RPL33B"
/transcript_id="NM_001183653.3"
/db_xref="GeneID:854409"
CDS complement(join(778555..778859,779387..779405))
/gene="RPL33B"
/locus_tag="YOR234C"
/gene_synonym="RPL37B"
/experiment="EXISTENCE:direct assay:GO:0002181 cytoplasmic
translation [PMID:18782943]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:18782943]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:18782943]"
/note="Ribosomal 60S subunit protein L33B; rpl33b null
mutant exhibits normal growth while rpl33a rpl33b double
null mutant is inviable; homologous to mammalian ribosomal
protein L35A, no bacterial homolog; RPL33B has a paralog,
RPL33A, that arose from the whole genome duplication"
/codon_start=1
/product="60S ribosomal protein eL33 RPL33B"
/protein_id="NP_014877.3"
/db_xref="GeneID:854409"
/db_xref="SGD:S000005760"
/translation="MAESHRLYVKGKHLSYQRSKRVNNPNVSLIKIEGVATPQEAQFY
LGKRIAYVYRASKEVRGSKIRVMWGKVTRTHGNSGVVRATFRNNLPAKTFGASVRIFL
YPSNI"
gene <779870..>780184
/gene="IRC13"
/locus_tag="YOR235W"
/db_xref="GeneID:854410"
mRNA <779870..>780184
/gene="IRC13"
/locus_tag="YOR235W"
/product="Irc13p"
/transcript_id="NM_001348879.1"
/db_xref="GeneID:854410"
CDS 779870..780184
/gene="IRC13"
/locus_tag="YOR235W"
/note="hypothetical protein; conserved across S.
cerevisiae strains; null mutant displays increased levels
of spontaneous Rad52 foci"
/codon_start=1
/product="Irc13p"
/protein_id="NP_001335817.1"
/db_xref="GeneID:854410"
/db_xref="SGD:S000005761"
/translation="MGLYRPSKFFHPPIPHIPFTINPDFFSFHIQRLKAKANPENFLI
CFPPPDIYKGFVFCCQLDLVHLFSYVFFLFLLKICVDVLQYVIYPKHFTHKKPGFENY
SI"
gene 780107..780596
/gene="SNR17A"
/locus_tag="YNCO0025W"
/db_xref="GeneID:9164981"
ncRNA 780107..780596
/ncRNA_class="snoRNA"
/gene="SNR17A"
/locus_tag="YNCO0025W"
/product="SNR17A"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:3327689]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:3327689|PMID:8455623]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911|PMID:15231838]"
/experiment="EXISTENCE:direct assay:GO:0031428 box C/D
methylation guide snoRNP complex
[PMID:10094313|PMID:11081632]"
/experiment="EXISTENCE:direct assay:GO:0032040
small-subunit processome [PMID:12068309]"
/experiment="EXISTENCE:genetic interaction:GO:0000447
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from
5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:7556076]"
/experiment="EXISTENCE:genetic interaction:GO:0000472
endonucleolytic cleavage to generate mature 5'-end of
SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:7556076]"
/experiment="EXISTENCE:genetic interaction:GO:0000480
endonucleolytic cleavage in 5'-ETS of tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:7556076]"
/experiment="EXISTENCE:genetic interaction:GO:0019843 rRNA
binding [PMID:7556076]"
/experiment="EXISTENCE:mutant phenotype:GO:0019843 rRNA
binding [PMID:7556076]"
/experiment="EXISTENCE:physical interaction:GO:0003723 RNA
binding [PMID:3327689]"
/experiment="EXISTENCE:physical interaction:GO:0031428 box
C/D methylation guide snoRNP complex [PMID:10733567]"
/note="Small nucleolar RNA (snoRNA) U3; part of Small
ribosomal SubUnit (SSU) processome; SNR17B also encodes
snoRNA U3"
/transcript_id="NR_132262.1"
/db_xref="GeneID:9164981"
/db_xref="SGD:S000007294"
gene <780906..>781541
/gene="DFR1"
/locus_tag="YOR236W"
/db_xref="GeneID:854411"
mRNA <780906..>781541
/gene="DFR1"
/locus_tag="YOR236W"
/product="dihydrofolate reductase"
/transcript_id="NM_001183655.1"
/db_xref="GeneID:854411"
CDS 780906..781541
/gene="DFR1"
/locus_tag="YOR236W"
/EC_number="1.5.1.3"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:21124907]"
/experiment="EXISTENCE:direct assay:GO:0004146
dihydrofolate reductase activity [PMID:2838385]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0004146
dihydrofolate reductase activity
[PMID:2827121|PMID:1427091]"
/experiment="EXISTENCE:mutant phenotype:GO:0046452
dihydrofolate metabolic process [PMID:1427091]"
/experiment="EXISTENCE:mutant phenotype:GO:0046654
tetrahydrofolate biosynthetic process [PMID:1427091]"
/note="Dihydrofolate reductase involved in
tetrahydrofolate biosynthesis; required for respiratory
metabolism; mutation is functionally complemented by human
DHFR"
/codon_start=1
/product="dihydrofolate reductase"
/protein_id="NP_014879.1"
/db_xref="GeneID:854411"
/db_xref="SGD:S000005762"
/translation="MAGGKIPIVGIVACLQPEMGIGFRGGLPWRLPSEMKYFRQVTSL
TKDPNKKNALIMGRKTWESIPPKFRPLPNRMNVIISRSFKDDFVHDKERSIVQSNSLA
NAIMNLESNFKEHLERIYVIGGGEVYSQIFSITDHWLITKINPLDKNATPAMDTFLDA
KKLEEVFSEQDPAQLKEFLPPKVELPETDCDQRYSLEEKGYCFEFTLYNRK"
gene <781994..>783298
/gene="HES1"
/locus_tag="YOR237W"
/gene_synonym="OSH5"
/db_xref="GeneID:854412"
mRNA <781994..>783298
/gene="HES1"
/locus_tag="YOR237W"
/gene_synonym="OSH5"
/product="oxysterol-binding protein related protein HES1"
/transcript_id="NM_001183656.3"
/db_xref="GeneID:854412"
CDS 781994..783298
/gene="HES1"
/locus_tag="YOR237W"
/gene_synonym="OSH5"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0008142 oxysterol
binding [PMID:16408938]"
/experiment="EXISTENCE:direct assay:GO:0008289 lipid
binding [PMID:20008566]"
/experiment="EXISTENCE:direct assay:GO:0015918 sterol
transport [PMID:20008566]"
/experiment="EXISTENCE:direct assay:GO:0120015 sterol
transfer activity [PMID:20008566]"
/experiment="EXISTENCE:genetic interaction:GO:0006887
exocytosis [PMID:17004323]"
/experiment="EXISTENCE:genetic interaction:GO:0006897
endocytosis [PMID:15173322]"
/experiment="EXISTENCE:genetic interaction:GO:0015918
sterol transport [PMID:16585271]"
/experiment="EXISTENCE:genetic interaction:GO:0030011
maintenance of cell polarity [PMID:17004323]"
/experiment="EXISTENCE:genetic interaction:GO:0034727
piecemeal microautophagy of the nucleus [PMID:15367582]"
/experiment="EXISTENCE:mutant phenotype:GO:0015918 sterol
transport [PMID:16585271]"
/note="Protein implicated in the regulation of ergosterol
biosynthesis; one of a seven member gene family with a
common essential function and non-essential unique
functions; similar to human oxysterol binding protein
(OSBP); SWAT-GFP and mCherry fusion proteins localize to
the bud neck and vacuolar membrane; HES1 has a paralog,
KES1, that arose from the whole genome duplication"
/codon_start=1
/product="oxysterol-binding protein related protein HES1"
/protein_id="NP_014880.3"
/db_xref="GeneID:854412"
/db_xref="SGD:S000005763"
/translation="MSQHASSSSWTSFLKSISSFNGDLSSLSAPPFILSPTSLTEFSQ
YWAEHPALFLEPSLIDGENYKDHCPFDPNVESKEVAQMLAVVRWFISTLRSQYCSRSE
SMGSEKKPLNPFLGEVFVGKWKNDEHPEFGETVLLSEQVSHHPPMTAFSIFNEKNDVS
VQGYNQIKTGFTKTLTLTVKPYGHVILKIKDETYLITTPPLHIEGILVASPFVELGGR
SFIQSSNGMLCVIEFSGRGYFTGKKNSFKARIYRSPQEHSHKENALYLISGQWSGVST
IIKKDSQVSHQFYDSSETPTEHLLVKPIEEQHPLESRRAWKDVAEAIRQGNISMIKKT
KEELENKQRALREQERVKGVEWQRRWFKQVDYMNENTSNDVEKASEDDAFRKLASKLQ
LSVKNVPSGTLIGGKDDKKDVSTALHWRFDKNLWMRENEITI"
rep_origin 783345..783563
/note="ARS1524; Autonomously Replicating Sequence"
/db_xref="SGD:S000118493"
gene <783677..>784588
/locus_tag="YOR238W"
/db_xref="GeneID:854413"
mRNA <783677..>784588
/locus_tag="YOR238W"
/product="uncharacterized protein"
/transcript_id="NM_001183657.1"
/db_xref="GeneID:854413"
CDS 783677..784588
/locus_tag="YOR238W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to the cytoplasm"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014881.2"
/db_xref="GeneID:854413"
/db_xref="SGD:S000005764"
/translation="MDEKVELILVPCHSIWKSSSHPSDNSVNLGQLPEYWHLAPFQYE
GNDHLAFIKHGLTAIKLLLQRFDTATVIFSGSQTKKEAGAISEAQSYYFLFEKLIRYV
MSNDNIDVPNFDNELRLLLKEVKNLLSSQNVNVDELFYGGSITTEEFSLDSFDNLIYS
IYRFEEVNKKFPQKITIIGFAFKMPRFISCHAKAIDYPQSNITYIGIDPKPANYNQTQ
LSKYYDDLVQMEDKNALSLFSSDWYATKDRLLTKKRSRNPFNRTAPYAQNIFCKENGK
RIEGIEDDEEYFETKIKCKMPWSSPRQ"
gene <784857..>786744
/gene="ABP140"
/locus_tag="YOR239W"
/gene_synonym="TRM140; YOR240W"
/db_xref="GeneID:854414"
mRNA <784857..>786744
/gene="ABP140"
/locus_tag="YOR239W"
/gene_synonym="TRM140; YOR240W"
/product="tRNA(Thr) (cytosine(32)-N(3))-methyltransferase"
/transcript_id="NM_001183658.6"
/db_xref="GeneID:854414"
CDS join(784857..785687,785689..786744)
/gene="ABP140"
/locus_tag="YOR239W"
/gene_synonym="TRM140; YOR240W"
/EC_number="2.1.1.268"
/experiment="EXISTENCE:direct assay:GO:0005884 actin
filament [PMID:9467951]"
/experiment="EXISTENCE:direct assay:GO:0030674
protein-macromolecule adaptor activity [PMID:9467951]"
/experiment="EXISTENCE:direct assay:GO:0032432 actin
filament bundle [PMID:11805329]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807]"
/experiment="EXISTENCE:direct assay:GO:0051015 actin
filament binding [PMID:9467951]"
/experiment="EXISTENCE:direct assay:GO:0052735 tRNA
(cytidine-3-)-methyltransferase activity
[PMID:21518805|PMID:21518804]"
/experiment="EXISTENCE:mutant phenotype:GO:0030488 tRNA
methylation [PMID:21518804|PMID:21518805]"
/experiment="EXISTENCE:mutant phenotype:GO:0052735 tRNA
(cytidine-3-)-methyltransferase activity [PMID:21518804]"
/ribosomal_slippage
/note="AdoMet-dependent tRNA methyltransferase and actin
binding protein; C-terminal domain is responsible for
3-methylcytidine modification of residue 32 of the tRNA
anticodon loop of tRNA-Thr and tRNA-Ser and contains an
S-adenosylmethionine (AdoMet) binding motif; N-terminal
actin binding sequence interacts with actin filaments and
localizes to actin patches and cables; N- and C-terminal
domains are encoded in separate ORFs that are translated
into one protein via a +1 frameshift"
/codon_start=1
/product="tRNA(Thr) (cytosine(32)-N(3))-methyltransferase"
/protein_id="NP_014882.4"
/db_xref="GeneID:854414"
/db_xref="SGD:S000005765"
/translation="MGVADLIKKFESISKEEGDATVDTNSSSKPLKSNDETKELHQQE
STAVPQEVDVNEEFENEPETINSSRTAEKPLETNLPKPETNEEDEEEGSMSENKIYSK
GENADINVNDFQEYKEMENTGAEVLASSVEESDAIQEGVAEETEGIATPKQKENEKND
ESEEESANNASEPAEEYSQSEEDADIEQSNGKETENAENASQQANDGSTSTTTSKNKK
KKNKKKNKKKRNGNVNTNANVDDSTKTGENDDTTGDTTSSTTSAIQEVNDLEVVDDSC
LGIDQQHNREHLKALTQDVKEETLENIAHEGRGDNTGDQNAVEKSDFEKSDTEGSRIG
RDLPFEFGKRNLTEESDVWDHNAWDNVEWGEEQVQQAEEKIKEQFKHPVPEFDKKLYN
ENPARYWDIFYKNNKENFFKDRKWLQIEFPILYASTRKDAEPVTIFEIGCGAGNTFFP
ILKDNENENLRIIAADFAPRAVELVKNSEQFNPKYGHATVWDLANPDGNLPDGVEPHS
VDIAVMIFVFSALAPNQWDQAMDNLHKILKPGGKIIFRDYGAYDLTQVRFKKNRILEE
NFYVRGDGTRVYFFSEEKLREIFTKKYFLENKIGTDRRLLVNRKRQLKMYRCWVQAVF
DVPQ"
gene <786995..>788641
/gene="MET7"
/locus_tag="YOR241W"
/gene_synonym="MET23"
/db_xref="GeneID:854415"
mRNA <786995..>788641
/gene="MET7"
/locus_tag="YOR241W"
/gene_synonym="MET23"
/product="tetrahydrofolate synthase"
/transcript_id="NM_001183660.1"
/db_xref="GeneID:854415"
CDS 786995..788641
/gene="MET7"
/locus_tag="YOR241W"
/gene_synonym="MET23"
/EC_number="6.3.2.17"
/experiment="EXISTENCE:direct assay:GO:0004326
tetrahydrofolylpolyglutamate synthase activity
[PMID:10775416]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:10775416]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:10775416]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0006730
one-carbon metabolic process [PMID:10775416]"
/note="Folylpolyglutamate synthetase; catalyzes extension
of the glutamate chains of the folate coenzymes, required
for methionine synthesis and for maintenance of
mitochondrial DNA; protein abundance increases in response
to DNA replication stress"
/codon_start=1
/product="tetrahydrofolate synthase"
/protein_id="NP_014884.1"
/db_xref="GeneID:854415"
/db_xref="SGD:S000005767"
/translation="MHKGKKNYPNLITSFRMNLKKIILNHDRFSHPERWKTNALLRFT
FVYIKFLFDLMIIKNPLRMVGKTYRDAVTALNSLQSNYANIMAIRQTGDRKNTMTLLE
MHEWSRRIGYSASDFNKLNIVHITGTKGKGSTAAFTSSILGQYKEQLPRIGLYTSPHL
KSVRERIRINGEPISEEKFAKYFFEVWDRLDSTTSSLDKFPHMIPGSKPGYFKFLTLL
SFHTFIQEDCKSCVYEVGVGGELDSTNIIEKPIVCGVTLLGIDHTFMLGDTIEEIAWN
KGGIFKSGAPAFTVEKQPPQGLTILKERAEERKTTLTEVPPFKQLENVKLGIAGEFQK
SNASLAVMLASEILHTSNILEEKIKCSSNASIPEKFIIGLQNTKWEGRCQVLEKGKNV
WYIDGAHTKDSMVAASTWFRDMVRLSKRKKILLFNQQSRDANALVNNLYSSVSPEITF
DDVIFTTNVTWKSGSYSADLVSMNTSQEDVEKLKVQESLVKNWNKIDDNRAKTHVTAS
IEEANELIETLYDEPADIFVTGSLHLVGGLLVVFDRIDVK"
gene complement(<788742..>789857)
/gene="SSP2"
/locus_tag="YOR242C"
/db_xref="GeneID:854416"
mRNA complement(<788742..>789857)
/gene="SSP2"
/locus_tag="YOR242C"
/product="Ssp2p"
/transcript_id="NM_001183661.3"
/db_xref="GeneID:854416"
CDS complement(788742..789857)
/gene="SSP2"
/locus_tag="YOR242C"
/experiment="EXISTENCE:direct assay:GO:0005619 ascospore
wall [PMID:12073037]"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0030295 protein
kinase activator activity [PMID:28223369]"
/experiment="EXISTENCE:direct assay:GO:0031954 positive
regulation of protein autophosphorylation [PMID:23207907]"
/experiment="EXISTENCE:mutant phenotype:GO:0030437
ascospore formation [PMID:17110477]"
/experiment="EXISTENCE:mutant phenotype:GO:0030476
ascospore wall assembly [PMID:12073037]"
/experiment="EXISTENCE:mutant phenotype:GO:0031954
positive regulation of protein autophosphorylation
[PMID:23207907]"
/note="Sporulation specific protein that localizes to the
spore wall; required for sporulation at a point after
meiosis II and during spore wall formation; expression
controlled by a tightly regulated middle-meiotic promoter
that is activated by Ndt80p; translation of SSP2 mRNA is
delayed, such that the mRNA is present as nuclear
divisions are taking place but is not engaged by ribosomes
until relatively late in meiotic development"
/codon_start=1
/product="Ssp2p"
/protein_id="NP_014885.3"
/db_xref="GeneID:854416"
/db_xref="SGD:S000005768"
/translation="MYKNYYSNTEVYKKHKDSGSLRKKALRSRRSSFFSFFNDSSSSN
GNEFIGFRRFAKAYLFGREIGSCGTDSYTPVGANVNKRRLKKEDKNDQQLWKRQHHSQ
GCFFPIDDDSNKQTEAAVNKFYENGEYVNQDLIFKGKVYSEESEVVDEKTAGSQNPAL
LKTRSISLNDIPRGTGISSVLSQVRGGSLERIIVYRYDTPERSLHKVDLFFLNYEGAQ
SFMRYAKTNIFKVNGVQLKPEWIFLESTYENIMKEQSVNRIIEEEKFISRCLIVKKSS
TTAMPNKSNLNKGQTLENIDIQELEKDFQNFGEVLEITPIVSRKLCVSIFFYDISSAM
RAMEEYEQKGSYLYNKYFKTWTIWYGKDITDQPCIDL"
gene complement(<790211..>792241)
/gene="PUS7"
/locus_tag="YOR243C"
/db_xref="GeneID:854417"
mRNA complement(<790211..>792241)
/gene="PUS7"
/locus_tag="YOR243C"
/product="pseudouridine synthase PUS7"
/transcript_id="NM_001183662.1"
/db_xref="GeneID:854417"
CDS complement(790211..792241)
/gene="PUS7"
/locus_tag="YOR243C"
/EC_number="5.4.99.27"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095|PMID:25219674]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:25219674]"
/experiment="EXISTENCE:direct assay:GO:0009982
pseudouridine synthase activity [PMID:12682021]"
/experiment="EXISTENCE:direct assay:GO:0031120 snRNA
pseudouridine synthesis [PMID:12682021]"
/experiment="EXISTENCE:mutant phenotype:GO:0000455
enzyme-directed rRNA pseudouridine synthesis
[PMID:18332121]"
/experiment="EXISTENCE:mutant phenotype:GO:0009982
pseudouridine synthase activity
[PMID:12682021|PMID:18332121]"
/experiment="EXISTENCE:mutant phenotype:GO:0031119 tRNA
pseudouridine synthesis [PMID:14561887]"
/experiment="EXISTENCE:mutant phenotype:GO:0031120 snRNA
pseudouridine synthesis [PMID:14561887|PMID:12682021]"
/experiment="EXISTENCE:mutant phenotype:GO:1990481 mRNA
pseudouridine synthesis [PMID:25219674|PMID:25192136]"
/note="Pseudouridine synthase; catalyzes pseudouridylation
at positions 35 and 56 in U2 snRNA, position 50 in 5S
rRNA, position 13 in cytoplasmic tRNAs, and position 35 in
pre-tRNA(Tyr); also pseudouridylates some mRNAs; relocates
from nucleus to cytoplasm during heat shock and
differentially modifies some mRNAs during heat shock;
conserved in archaea, vertebrates, and some bacteria"
/codon_start=1
/product="pseudouridine synthase PUS7"
/protein_id="NP_014886.1"
/db_xref="GeneID:854417"
/db_xref="SGD:S000005769"
/translation="MSDSSEATVKRPLDAHVGPSENAAKKLKIEQRTQADGIHEADVG
ITLFLSPELPGFRGQIKQRYTDFLVNEIDQEGKVIHLTDKGFKMPKKPQRSKEEVNAE
KESEAARRQEFNVDPELRNQLVEIFGEEDVLKIESVYRTANKMETAKNFEDKSVRTKI
HQLLREAFKNELESVTTDTNTFKIARSNRNSRTNKQEKINQTRDANGVENWGYGPSKD
FIHFTLHKENKDTMEAVNVITKLLRVPSRVIRYAGTKDRRAVTCQRVSISKIGLDRLN
ALNRTLKGMIIGNYNFSDASLNLGDLKGNEFVVVIRDVTTGNSEVSLEEIVSNGCKSL
SENGFINYFGMQRFGTFSISTHTIGRELLLSNWKKAAELILSDQDNVLPKSKEARKIW
AETKDAALALKQMPRQCLAENALLYSLSNQRKEEDGTYSENAYYTAIMKIPRNLRTMY
VHAYQSYVWNSIASKRIELHGLKLVVGDLVIDTSEKSPLISGIDDEDFDEDVREAQFI
RAKAVTQEDIDSVKYTMEDVVLPSPGFDVLYPSNEELKQLYVDILKADNMDPFNMRRK
VRDFSLAGSYRTVIQKPKSLEYRIIHYDDPSQQLVNTDLDILNNTRAKESGQKYMKAK
LDRYMPDKGGEKTAVVLKFQLGTSAYATMALRELMKLETSRRGDMCDVKENI"
gene <792531..>793868
/gene="ESA1"
/locus_tag="YOR244W"
/gene_synonym="KAT5; TAS1"
/db_xref="GeneID:854418"
mRNA <792531..>793868
/gene="ESA1"
/locus_tag="YOR244W"
/gene_synonym="KAT5; TAS1"
/product="NuA4 histone acetyltransferase complex catalytic
subunit ESA1"
/transcript_id="NM_001183663.3"
/db_xref="GeneID:854418"
CDS 792531..793868
/gene="ESA1"
/locus_tag="YOR244W"
/gene_synonym="KAT5; TAS1"
/EC_number="2.3.1.48"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:10911987]"
/experiment="EXISTENCE:direct assay:GO:0003712
transcription coregulator activity [PMID:31699900]"
/experiment="EXISTENCE:direct assay:GO:0004402 histone
acetyltransferase activity [PMID:17274630]"
/experiment="EXISTENCE:direct assay:GO:0006281 DNA repair
[PMID:16135807]"
/experiment="EXISTENCE:direct assay:GO:0006354
DNA-templated transcription elongation [PMID:15949446]"
/experiment="EXISTENCE:direct assay:GO:0010485 histone H4
acetyltransferase activity [PMID:12110674]"
/experiment="EXISTENCE:direct assay:GO:0032777 piccolo
histone acetyltransferase complex [PMID:12782659]"
/experiment="EXISTENCE:direct assay:GO:0035267 NuA4
histone acetyltransferase complex
[PMID:10911987|PMID:15485911]"
/experiment="EXISTENCE:direct assay:GO:0140068 histone
crotonyltransferase activity [PMID:31699900]"
/experiment="EXISTENCE:genetic interaction:GO:0000183 rDNA
heterochromatin formation [PMID:16436512]"
/experiment="EXISTENCE:genetic interaction:GO:0006281 DNA
repair [PMID:25628362]"
/experiment="EXISTENCE:genetic interaction:GO:0032968
positive regulation of transcription elongation by RNA
polymerase II [PMID:19822662]"
/experiment="EXISTENCE:mutant phenotype:GO:0000183 rDNA
heterochromatin formation [PMID:16436512]"
/experiment="EXISTENCE:mutant phenotype:GO:0004402 histone
acetyltransferase activity [PMID:10487762]"
/experiment="EXISTENCE:mutant phenotype:GO:0006281 DNA
repair [PMID:12353039]"
/experiment="EXISTENCE:mutant phenotype:GO:0006354
DNA-templated transcription elongation [PMID:15949446]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:11036083]"
/experiment="EXISTENCE:mutant phenotype:GO:0016239
positive regulation of macroautophagy [PMID:22539722]"
/experiment="EXISTENCE:mutant phenotype:GO:0032968
positive regulation of transcription elongation by RNA
polymerase II [PMID:19822662]"
/experiment="EXISTENCE:mutant phenotype:GO:0034212 protein
N-acetyltransferase activity [PMID:23050233]"
/experiment="EXISTENCE:mutant phenotype:GO:0051726
regulation of cell cycle [PMID:10082517]"
/note="Catalytic subunit of the histone acetyltransferase
complex (NuA4); acetylates four conserved internal lysines
of histone H4 N-terminal tail and can acetylate histone
H2A; master regulator of cellular acetylation balance;
required for cell cycle progression and transcriptional
silencing at the rDNA locus and regulation of autophagy;
human ortholog TIP60/KAT5 is implicated in cancer and
other diseases, functionally complements lethality of the
esa1 null mutation"
/codon_start=1
/product="NuA4 histone acetyltransferase complex catalytic
subunit ESA1"
/protein_id="NP_014887.3"
/db_xref="GeneID:854418"
/db_xref="SGD:S000005770"
/translation="MSHDGKEEPGIAKKINSVDDIIIKCQCWVQKNDEERLAEILSIN
TRKAPPKFYVHYVNYNKRLDEWITTDRINLDKEVLYPKLKATDEDNKKQKKKKATNTS
ETPQDSLQDGVDGFSRENTDVMDLDNLNVQGIKDENISHEDEIKKLRTSGSMTQNPHE
VARVRNLNRIIMGKYEIEPWYFSPYPIELTDEDFIYIDDFTLQYFGSKKQYERYRKKC
TLRHPPGNEIYRDDYVSFFEIDGRKQRTWCRNLCLLSKLFLDHKTLYYDVDPFLFYCM
TRRDELGHHLVGYFSKEKESADGYNVACILTLPQYQRMGYGKLLIEFSYELSKKENKV
GSPEKPLSDLGLLSYRAYWSDTLITLLVEHQKEITIDEISSMTSMTTTDILHTAKTLN
ILRYYKGQHIIFLNEDILDRYNRLKAKKRRTIDPNRLIWKPPVFTASQLRFAW"
gene complement(<794076..>795332)
/gene="DGA1"
/locus_tag="YOR245C"
/db_xref="GeneID:854419"
mRNA complement(<794076..>795332)
/gene="DGA1"
/locus_tag="YOR245C"
/product="diacylglycerol O-acyltransferase"
/transcript_id="NM_001183664.1"
/db_xref="GeneID:854419"
CDS complement(794076..795332)
/gene="DGA1"
/locus_tag="YOR245C"
/EC_number="2.3.1.20"
/EC_number="2.3.1.22"
/experiment="EXISTENCE:direct assay:GO:0004144
diacylglycerol O-acyltransferase activity [PMID:20225889]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005811 lipid
droplet [PMID:11751830|PMID:24868093]"
/experiment="EXISTENCE:direct assay:GO:0006672 ceramide
metabolic process [PMID:22738231]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:21321129]"
/experiment="EXISTENCE:direct assay:GO:0019432
triglyceride biosynthetic process [PMID:11751830]"
/experiment="EXISTENCE:mutant phenotype:GO:0004144
diacylglycerol O-acyltransferase activity [PMID:11751830]"
/experiment="EXISTENCE:mutant phenotype:GO:0006672
ceramide metabolic process [PMID:22738231]"
/experiment="EXISTENCE:mutant phenotype:GO:0019432
triglyceride biosynthetic process [PMID:11751830]"
/experiment="EXISTENCE:mutant phenotype:GO:0019915 lipid
storage [PMID:11751830]"
/experiment="EXISTENCE:mutant phenotype:GO:0035356
intracellular triglyceride homeostasis [PMID:27620384]"
/note="Diacylglycerol acyltransferase; catalyzes the
terminal step of triacylglycerol (TAG) formation, acylates
diacylglycerol using acyl-CoA as an acyl donor; Lro1p and
Dga1p can O-acylate ceramides; localized to lipid
particles"
/codon_start=1
/product="diacylglycerol O-acyltransferase"
/protein_id="NP_014888.1"
/db_xref="GeneID:854419"
/db_xref="SGD:S000005771"
/translation="MSGTFNDIRRRKKEEGSPTAGITERHENKSLSSIDKREQTLKPQ
LESCCPLATPFERRLQTLAVAWHTSSFVLFSIFTLFAISTPALWVLAIPYMIYFFFDR
SPATGEVVNRYSLRFRSLPIWKWYCDYFPISLIKTVNLKPTFTLSKNKRVNEKNYKIR
LWPTKYSINLKSNSTIDYRNQECTGPTYLFGYHPHGIGALGAFGAFATEGCNYSKIFP
GIPISLMTLVTQFHIPLYRDYLLALGISSVSRKNALRTLSKNQSICIVVGGARESLLS
STNGTQLILNKRKGFIKLAIQTGNINLVPVFAFGEVDCYNVLSTKKDSVLGKMQLWFK
ENFGFTIPIFYARGLFNYDFGLLPFRAPINVVVGRPIYVEKKITNPPDDVVNHFHDLY
IAELKRLYYENREKYGVPDAELKIVG"
gene complement(<795801..>796793)
/gene="ENV9"
/locus_tag="YOR246C"
/db_xref="GeneID:854420"
mRNA complement(<795801..>796793)
/gene="ENV9"
/locus_tag="YOR246C"
/product="Env9p"
/transcript_id="NM_001183665.3"
/db_xref="GeneID:854420"
CDS complement(795801..796793)
/gene="ENV9"
/locus_tag="YOR246C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:25546499]"
/experiment="EXISTENCE:direct assay:GO:0005811 lipid
droplet [PMID:28540421|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0016491
oxidoreductase activity [PMID:28540421]"
/experiment="EXISTENCE:mutant phenotype:GO:0006624
vacuolar protein processing [PMID:21912603]"
/experiment="EXISTENCE:mutant phenotype:GO:0007033 vacuole
organization [PMID:21912603]"
/experiment="EXISTENCE:mutant phenotype:GO:0034389 lipid
droplet organization [PMID:28540421]"
/note="Conserved oxidoreductase involved in lipid droplet
morphology; mutant shows defects in CPY processing and
vacuolar morphology; required for replication of Brome
mosaic virus in S. cerevisiae, a model system for studying
replication of positive-strand RNA viruses in their
natural hosts; homologus to human RDH12 linked to Leber
Congenital Amaurosis"
/codon_start=1
/product="Env9p"
/protein_id="NP_014889.3"
/db_xref="GeneID:854420"
/db_xref="SGD:S000005772"
/translation="MLDPRILPYYDPAVERKIAVVTGGNTGIGWYTVLHLYLHGFVVY
ICGRNSHKISKAIQEILAEAKKRCHEDDDGSSPGAGPGPSIQRLGSLHYIHLDLTDLK
CVERAALKILKLEDHIDVLVNNAGIMAVPLEMTKDGFEVQLQTNYISHFIFTMRLLPL
LRHCRGRIISLSSIGHHLEFMYWKLSKTWDYKPNMLFTWFRYAMSKTALIQCTKMLAI
KYPDVLCLSVHPGLVMNTNLFSYWTRLPIVGIFFWLLFQVVGFFFGVSNEQGSLASLK
CALDPNLSVEKDNGKYFTTGGKESKSSYVSNNVDEAASTWIWTVHQLRDRGFDI"
gene <797676..>798308
/gene="SRL1"
/locus_tag="YOR247W"
/db_xref="GeneID:854421"
mRNA <797676..>798308
/gene="SRL1"
/locus_tag="YOR247W"
/product="Srl1p"
/transcript_id="NM_001183666.1"
/db_xref="GeneID:854421"
CDS 797676..798308
/gene="SRL1"
/locus_tag="YOR247W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:13679573]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:11935221]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0006139
nucleobase-containing compound metabolic process
[PMID:9744871]"
/experiment="EXISTENCE:mutant phenotype:GO:0031505
fungal-type cell wall organization [PMID:15972461]"
/note="Mannoprotein that exhibits a tight association with
the cell wall; required for cell wall stability in the
absence of GPI-anchored mannoproteins; has a high
serine-threonine content; expression is induced in cell
wall mutants; SRL1 has a paralog, SVS1, that arose from
the whole genome duplication"
/codon_start=1
/product="Srl1p"
/protein_id="NP_014890.1"
/db_xref="GeneID:854421"
/db_xref="SGD:S000005773"
/translation="MLQSVVFFALLTFASSVSAIYSNNTVSTTTTLAPSYSLVPQETT
ISYADDTTTFFVTSTVYSTSWFTSTSATITNAASSSLSTSSASGSVTPESTHEITSTS
TITSTLLLTLHDSTTLSPSSTAASVSDEDSNNKDAKVKSFEQASTSNGCVPITKFVTV
TNEPVTQYVTVTPNTTTQYVTVTGAPSVTTTSPGNVQWYNTTSITNSTSW"
gene complement(<798674..>800731)
/gene="APC5"
/locus_tag="YOR249C"
/gene_synonym="RMC1"
/db_xref="GeneID:854423"
mRNA complement(<798674..>800731)
/gene="APC5"
/locus_tag="YOR249C"
/gene_synonym="RMC1"
/product="anaphase promoting complex subunit 5"
/transcript_id="NM_001183668.3"
/db_xref="GeneID:854423"
CDS complement(798674..800731)
/gene="APC5"
/locus_tag="YOR249C"
/gene_synonym="RMC1"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005680
anaphase-promoting complex [PMID:9469814]"
/experiment="EXISTENCE:direct assay:GO:0061630 ubiquitin
protein ligase activity [PMID:16481473]"
/experiment="EXISTENCE:mutant phenotype:GO:0006325
chromatin organization [PMID:12399376]"
/experiment="EXISTENCE:mutant phenotype:GO:0016567 protein
ubiquitination [PMID:16481473]"
/experiment="EXISTENCE:mutant phenotype:GO:0031145
anaphase-promoting complex-dependent catabolic process
[PMID:16481473]"
/note="Subunit of the Anaphase-Promoting Complex/Cyclosome
(APC/C); APC/C is a ubiquitin-protein ligase required for
degradation of anaphase inhibitors, including mitotic
cyclins, during the metaphase/anaphase transition;
component of the platform domain of the APC/C, based on
structural analysis; relative distribution to nuclear foci
decreases upon DNA replication stress"
/codon_start=1
/product="anaphase promoting complex subunit 5"
/protein_id="NP_014892.3"
/db_xref="GeneID:854423"
/db_xref="SGD:S000005775"
/translation="MSKYGPLGITNFITPYDLCILILIHAHCSQDNGISVPTAVFLRL
ISPTRPSLEWNPLLKDNSNLRSSSIVPPPVLPILDNIIRILLDDKDGNKIALTLMGYL
EAINGLDSINRLMMDLEKNCLVNNYRSMKMRTTSTRRQMTRASFLGTFLSTCIRKYQI
GDFEMRETIWINLQNFKTVFKHTPLWLRFKDNVHIQKVKNCLLANDEISVEDQQMVEF
FQHFNNGNDADSKTMNEENYGTLISIQHLQSIVNRQIVNWLDNTEFNLMGQEETSSTY
EEQSGLVFDLLDTLSLNDATKFPLIFILKYLEAIKENSYQTALDSLHNYFDYKSTGNS
QNYFHISLLSLATFHSSFNECDAAINSFEEATRIARENKDMETLNLIMIWIINFIEVH
PEYANRFYITVEQIIKYLKNSSDVEDANIFSNAYKFETLLSMVKESKTAEVSSSLLKF
MAITLQNVPSQNFDLFQSLVSYEVKFWKELGYESISDVYEKFLSKTSSSSLRNYDSSI
INQDIKVAFKALEEDDFLKVKQYLLKSESLELDYDQKINLKYLRVKYLVKIGDYDLSM
RLINQYVKECCEEVADSNWRFKFEIESINVLLLSDVGIRSLPKIIKLIDEYKEIGNPL
RCVILLLKLCEVLIQVGKSMEAECLISCNLSTILEFPFVRKKTDELLESLSVEEDRDV
QMT"
gene complement(<800970..>802307)
/gene="CLP1"
/locus_tag="YOR250C"
/db_xref="GeneID:854424"
mRNA complement(<800970..>802307)
/gene="CLP1"
/locus_tag="YOR250C"
/product="cleavage polyadenylation factor subunit CLP1"
/transcript_id="NM_001183669.1"
/db_xref="GeneID:854424"
CDS complement(800970..802307)
/gene="CLP1"
/locus_tag="YOR250C"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:11344258]"
/experiment="EXISTENCE:direct assay:GO:0031124 mRNA 3'-end
processing [PMID:11344258]"
/experiment="EXISTENCE:physical interaction:GO:0005849
mRNA cleavage factor complex [PMID:11344258]"
/note="Component of the cleavage and polyadenylation
factor I (CF I); CF 1, composed of the CF 1A complex
(Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in
cleavage and polyadenylation of mRNA 3' ends; involved in
both the endonucleolyitc cleavage and polyadenylation
steps of mRNA 3'-end maturation and in gene looping which
affects reinitiation of transcription; mutations in human
ortholog cause cerebellar neurodegeneration"
/codon_start=1
/product="cleavage polyadenylation factor subunit CLP1"
/protein_id="NP_014893.1"
/db_xref="GeneID:854424"
/db_xref="SGD:S000005776"
/translation="MASLPGIDEHTTSEELITGDNEWHKLVIPKGSDWQIDLKAEGKL
IVKVNSGIVEIFGTELAVDDEYTFQNWKFPIYAVEETELLWKCPDLTTNTITVKPNHT
MKYIYNLHFMLEKIRMSNFEGPRVVIVGGSQTGKTSLSRTLCSYALKFNAYQPLYINL
DPQQPIFTVPGCISATPISDILDAQLPTWGQSLTSGATLLHNKQPMVKNFGLERINEN
KDLYLECISQLGQVVGQRLHLDPQVRRSGCIVDTPSISQLDENLAELHHIIEKLNVNI
MLVLCSETDPLWEKVKKTFGPELGNNNIFFIPKLDGVSAVDDVYKRSLQRTSIREYFY
GSLDTALSPYAIGVDYEDLTIWKPSNVFDNEVGRVELFPVTITPSNLQHAIIAITFAE
RRADQATVIKSPILGFALITEVNEKRRKLRVLLPVPGRLPSKAMILTSYRYLE"
gene complement(<802550..>803464)
/gene="TUM1"
/locus_tag="YOR251C"
/db_xref="GeneID:854425"
mRNA complement(<802550..>803464)
/gene="TUM1"
/locus_tag="YOR251C"
/product="thiosulfate sulfurtransferase"
/transcript_id="NM_001183670.3"
/db_xref="GeneID:854425"
CDS complement(802550..803464)
/gene="TUM1"
/locus_tag="YOR251C"
/EC_number="2.8.1.1"
/experiment="EXISTENCE:direct assay:GO:0004792
thiosulfate-cyanide sulfurtransferase activity
[PMID:19151091]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239]"
/experiment="EXISTENCE:mutant phenotype:GO:0002098 tRNA
wobble uridine modification [PMID:18755837]"
/experiment="EXISTENCE:mutant phenotype:GO:0002143 tRNA
wobble position uridine thiolation
[PMID:19151091|PMID:19145231]"
/experiment="EXISTENCE:mutant phenotype:GO:0004792
thiosulfate-cyanide sulfurtransferase activity
[PMID:19151091]"
/note="Rhodanese domain sulfur transferase; accepts
persulfite from Nfs1p and transfers it to Uba4p in the
pathway for 2-thiolation of the wobble uridine base of
tRNAs; also stimulates sulfur transfer by Nfs1p; involved
in metabolism of sterol esters; may be mitochondrially
localized"
/codon_start=1
/product="thiosulfate sulfurtransferase"
/protein_id="NP_014894.3"
/db_xref="GeneID:854425"
/db_xref="SGD:S000005777"
/translation="MPLFDLISPKAFVKLVASEKVHRIVPVDATWYLPSWKLDNKVDF
LTKPRIPNSIFFDIDAISDKKSPYPHMFPTKKVFDDAMSNLGVQKDDILVVYDRVGNF
SSPRCAWTLGVMGHPKVYLLNNFNQYREFKYPLDSSKVAAFSPYPKSHYESSESFQDK
EIVDYEEMFQLVKSGELAKKFNAFDARSLGRFEGTEPEPRSDIPSGHIPGTQPLPYGS
LLDPETKTYPEAGEAIHATLEKALKDFHCTLDPSKPTICSCGTGVSGVIIKTALELAG
VPNVRLYDGSWTEWVLKSGPEWIAENRD"
gene <803667..>804203
/gene="TMA16"
/locus_tag="YOR252W"
/db_xref="GeneID:854426"
mRNA <803667..>804203
/gene="TMA16"
/locus_tag="YOR252W"
/product="Tma16p"
/transcript_id="NM_001183671.1"
/db_xref="GeneID:854426"
CDS 803667..804203
/gene="TMA16"
/locus_tag="YOR252W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/note="hypothetical protein that associates with
ribosomes"
/codon_start=1
/product="Tma16p"
/protein_id="NP_014895.2"
/db_xref="GeneID:854426"
/db_xref="SGD:S000005778"
/translation="MPVTKSLSKLQKNLSKKGKNITVHPKGRKYEKLVRATMREDKIA
AKKKLHQDKRVHELARVKFMQDVVNSDTFKGQPIFDHAHTREFIQSFIERDDTELDEL
KKKRRSNRPPSNRQVLLQQRRDQELKEFKAGFLCPDLSDAKNMEFLRNWNGTFGLLNT
LRLIRINDKGEQVVGGNE"
gene <804377..>804907
/gene="NAT5"
/locus_tag="YOR253W"
/gene_synonym="ARD2; NAA50; ROG2"
/db_xref="GeneID:854427"
mRNA <804377..>804907
/gene="NAT5"
/locus_tag="YOR253W"
/gene_synonym="ARD2; NAA50; ROG2"
/product="peptide alpha-N-acetyltransferase subunit NAT5"
/transcript_id="NM_001183672.3"
/db_xref="GeneID:854427"
CDS 804377..804907
/gene="NAT5"
/locus_tag="YOR253W"
/gene_synonym="ARD2; NAA50; ROG2"
/EC_number="2.3.1.258"
/experiment="EXISTENCE:direct assay:GO:0031415 NatA
complex [PMID:14517307]"
/experiment="EXISTENCE:mutant phenotype:GO:0004596
protein-N-terminal amino-acid acetyltransferase activity
[PMID:25886145]"
/experiment="EXISTENCE:mutant phenotype:GO:0006474
N-terminal protein amino acid acetylation [PMID:25886145]"
/note="Subunit of protein N-terminal acetyltransferase
NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p;
N-terminally acetylates many proteins, which influences
multiple processes such as the cell cycle, heat-shock
resistance, mating, sporulation, and telomeric silencing"
/codon_start=1
/product="peptide alpha-N-acetyltransferase subunit NAT5"
/protein_id="NP_014896.3"
/db_xref="GeneID:854427"
/db_xref="SGD:S000005779"
/translation="MGRDICTLDNVYANNLGMLTKLAHVTVPNLYQDAFFSALFAEDS
LVAKNKKPSSKKDVHFTQMAYYSEIPVGGLVAKLVPKKQNELSLKGIQIEFLGVLPNY
RHKSIGSKLLKFAEDKCSECHQHNVFVYLPAVDDLTKQWFIAHGFEQVGETVNNFIKG
VNGDEQDAILLKKHIS"
gene complement(<805032..>807023)
/gene="SEC63"
/locus_tag="YOR254C"
/gene_synonym="PTL1"
/db_xref="GeneID:854428"
mRNA complement(<805032..>807023)
/gene="SEC63"
/locus_tag="YOR254C"
/gene_synonym="PTL1"
/product="protein-transporting protein SEC63"
/transcript_id="NM_001183673.1"
/db_xref="GeneID:854428"
CDS complement(805032..807023)
/gene="SEC63"
/locus_tag="YOR254C"
/gene_synonym="PTL1"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762|PMID:14562095|PMID:27831485]"
/experiment="EXISTENCE:direct assay:GO:0008320 protein
transmembrane transporter activity [PMID:9252322]"
/experiment="EXISTENCE:direct assay:GO:0031204
post-translational protein targeting to membrane,
translocation [PMID:7758110]"
/experiment="EXISTENCE:mutant phenotype:GO:0006614
SRP-dependent cotranslational protein targeting to
membrane [PMID:11226176]"
/experiment="EXISTENCE:mutant phenotype:GO:0006620
post-translational protein targeting to endoplasmic
reticulum membrane [PMID:15671059]"
/experiment="EXISTENCE:mutant phenotype:GO:0008320 protein
transmembrane transporter activity [PMID:15671059]"
/experiment="EXISTENCE:mutant phenotype:GO:0046967 cytosol
to endoplasmic reticulum transport [PMID:15671059]"
/experiment="EXISTENCE:physical interaction:GO:0031207
Sec62/Sec63 complex [PMID:2000150]"
/note="Essential subunit of Sec63 complex; with Sec61
complex, Kar2p/BiP and Lhs1p forms a channel competent for
SRP-dependent and post-translational SRP-independent
protein targeting and import into the ER; other members
are Sec62p, Sec66p, and Sec72p"
/codon_start=1
/product="protein-transporting protein SEC63"
/protein_id="NP_014897.1"
/db_xref="GeneID:854428"
/db_xref="SGD:S000005780"
/translation="MPTNYEYDEASETWPSFILTGLLMVVGPMTLLQIYQIFFGANAE
DGNSGKSKEFNEEVFKNLNEEYTSDEIKQFRRKFDKNSNKKSKIWSRRNIIIIVGWIL
VAILLQRINSNDAIKDAATKLFDPYEILGISTSASDRDIKSAYRKLSVKFHPDKLAKG
LTPDEKSVMEETYVQITKAYESLTDELVRQNYLKYGHPDGPQSTSHGIALPRFLVDGS
ASPLLVVCYVALLGLILPYFVSRWWARTQSYTKKGIHNVTASNFVSNLVNYKPSEIVT
TDLILHWLSFAHEFKQFFPDLQPTDFEKLLQDHINRRDSGKLNNAKFRIVAKCHSLLH
GLLDIACGFRNLDIALGAINTFKCIVQAVPLTPNCQILQLPNVDKEHFITKTGDIHTL
GKLFTLEDAKIGEVLGIKDQAKLNETLRVASHIPNLKIIKADFLVPGENQVTPSSTPY
ISLKVLVRSAKQPLIPTSLIPEENLTEPQDFESQRDPFAMMSKQPLVPYSFAPFFPTK
RRGSWCCLVSSQKDGKILQTPIIIEKLSYKNLNDDKDFFDKRIKMDLTKHEKFDINDW
EIGTIKIPLGQPAPETVGDFFFRVIVKSTDYFTTDLDITMNMKVRDSPAVEQVEVYSE
EDDEYSTDDDETESDDESDASDYTDIDTDTEAEDDESPE"
gene <807271..>808107
/gene="OSW1"
/locus_tag="YOR255W"
/db_xref="GeneID:854429"
mRNA <807271..>808107
/gene="OSW1"
/locus_tag="YOR255W"
/product="Osw1p"
/transcript_id="NM_001183674.3"
/db_xref="GeneID:854429"
CDS 807271..808107
/gene="OSW1"
/locus_tag="YOR255W"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:15590821]"
/experiment="EXISTENCE:mutant phenotype:GO:0030437
ascospore formation [PMID:17110477]"
/experiment="EXISTENCE:mutant phenotype:GO:0030476
ascospore wall assembly [PMID:15590821]"
/note="Protein involved in sporulation; required for the
construction of the outer spore wall layers; required for
proper localization of Spo14p"
/codon_start=1
/product="Osw1p"
/protein_id="NP_014898.3"
/db_xref="GeneID:854429"
/db_xref="SGD:S000005781"
/translation="MRAPPSPRKSKSGHFFYLYFRLCQLFSGRKLKRRWHVHKLHIHQ
YNTRWNLSPLSEIHIEDMINEPSGLCPGSSKKKPLLIARFPKGCQESPRVYVLQRNNL
SRLKLSKRKYALRFYHNEIFGNNLKRKDGSIHKVEHQQCAETVRKIKKVTANHADVKI
IFHDKNTIRSDKLGGRSNKMQTRPSVLEEDVEEEVSSVYIRFCDDHSLRVKDYHSLHR
HSKKSSKEKRNNQEIGKSKLLGKLFEEETSRQNKGVEKKLDTIVIQKFQNYPIVSFSR
VI"
gene complement(<808254..>810683)
/gene="TRE2"
/locus_tag="YOR256C"
/db_xref="GeneID:854430"
mRNA complement(<808254..>810683)
/gene="TRE2"
/locus_tag="YOR256C"
/product="putative zinc metalloprotease"
/transcript_id="NM_001183675.2"
/db_xref="GeneID:854430"
CDS complement(808254..810683)
/gene="TRE2"
/locus_tag="YOR256C"
/experiment="EXISTENCE:genetic interaction:GO:0043328
protein transport to vacuole involved in
ubiquitin-dependent protein catabolic process via the
multivesicular body sorting pathway [PMID:19369420]"
/note="Transferrin receptor-like protein; functions with
Tre1p to regulate ubiquitination and vacuolar degradation
of the metal transporter Smf1p; inviability of null mutant
in systematic studies is due to proximity to CDC31; TRE2
has a paralog, TRE1, that arose from the whole genome
duplication"
/codon_start=1
/product="putative zinc metalloprotease"
/protein_id="NP_014899.1"
/db_xref="GeneID:854430"
/db_xref="SGD:S000005782"
/translation="MRSSYQPVSTTNFEHENAIPTASSSHNLLMSQRFDDSPPSSNDN
SIETNITPPPEPPSYEFDIEDPHDDLHKRTHLQRVSIGFQEKILEPLMENIIHPLLQI
SKFVPDKADYYLSKIGNPFILRRFFYIIFMSFIAYYVLSSGYLFNEKASGSKGMFSQH
DILFEYAKKSVDLAKFERDLEYISSMPHGSGTKGDAAIYRYIQESFDNNGLKLVKEMG
YSVYSNYPGNVSISYYDNKNEKHDLELSKENFNPLSSNGKLSKVSLIYGGKGTTYDLQ
HLKDSKTIEDGKDYVLLLQYDKLVSQQVLIAEKFGAKAVIFISEPYGENIDVVQSKPV
GLPQYSTGDASGLNWDGSPVEEKDHKFWRQTHIPTIPISTRQGKELLSRLSSGGVTVD
DGNSDRSNSGKMGDVLIDVDLQTNVREKHFIPNIVGKIEGREQSDKAIIIAASRNSIN
FGTTYPNFGTAALLSIVQLFQEVKYKFGWKPLRNIYFISFGGTEFNYAGSSELVEQRL
TPLKDEIYSLIDISQLGIPFAEKYENGKTRGELSIETHPLLKKFFNRNAHGNFDISVD
NVQHYGDWTPFLANGIPVSVISSDSTRNRDTPTETSEDKFERVEKILEDEQNQQSVKD
LLVYLLHISMELIDDPLLHFDIISYVEDIDERLQRLEQAYPEKLNFTSIIKGLLFWKK
IGSEWASWTQGWENIVWSHGDGIEPSLLSINRWTWNKKLTNIGRRTCSPAGLPNRSFY
KNVLFGPTLIQEDKSKNGGNVDFWTFPGVMDAIYDDDWKRAQEQIDLIGKVLHQSAAL
FVEETNDIGYK"
gene <811008..>811493
/gene="CDC31"
/locus_tag="YOR257W"
/gene_synonym="DSK1"
/db_xref="GeneID:854431"
mRNA <811008..>811493
/gene="CDC31"
/locus_tag="YOR257W"
/gene_synonym="DSK1"
/product="centrin"
/transcript_id="NM_001183676.3"
/db_xref="GeneID:854431"
CDS 811008..811493
/gene="CDC31"
/locus_tag="YOR257W"
/gene_synonym="DSK1"
/experiment="EXISTENCE:direct assay:GO:0005825 half bridge
of spindle pole body [PMID:8408222]"
/experiment="EXISTENCE:direct assay:GO:0070390
transcription export complex 2 [PMID:15311284]"
/experiment="EXISTENCE:mutant phenotype:GO:0030474 spindle
pole body duplication [PMID:9153752]"
/experiment="EXISTENCE:mutant phenotype:GO:0043161
proteasome-mediated ubiquitin-dependent protein catabolic
process [PMID:18160718]"
/note="Calcium-binding component of the spindle pole body
(SPB) half-bridge; required for SPB duplication in mitosis
and meiosis II; homolog of mammalian centrin; binds
multiubiquitinated proteins and is involved in proteasomal
protein degradation"
/codon_start=1
/product="centrin"
/protein_id="NP_014900.3"
/db_xref="GeneID:854431"
/db_xref="SGD:S000005783"
/translation="MSKNRSSLQSGPLNSELLEEQKQEIYEAFSLFDMNNDGFLDYHE
LKVAMKALGFELPKREILDLIDEYDSEGRHLMKYDDFYIVMGEKILKRDPLDEIKRAF
QLFDDDHTGKISIKNLRRVAKELGETLTDEELRAMIEEFDLDGDGEINENEFIAICTD
S"
gene <811671..>812324
/gene="HNT3"
/locus_tag="YOR258W"
/db_xref="GeneID:854432"
mRNA <811671..>812324
/gene="HNT3"
/locus_tag="YOR258W"
/product="DNA 5'-adenosine monophosphate hydrolase"
/transcript_id="NM_001183677.1"
/db_xref="GeneID:854432"
CDS 811671..812324
/gene="HNT3"
/locus_tag="YOR258W"
/EC_number="3.6.1.71"
/EC_number="3.6.1.72"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0033699 DNA
5'-adenosine monophosphate hydrolase activity
[PMID:16964241]"
/experiment="EXISTENCE:genetic interaction:GO:0006974 DNA
damage response [PMID:20399152]"
/experiment="EXISTENCE:mutant phenotype:GO:0006974 DNA
damage response [PMID:20399152]"
/note="DNA 5' AMP hydrolase involved in DNA repair; member
of the histidine triad (HIT) superfamily of
nucleotide-binding proteins; homolog of Aprataxin, a Hint
related protein that is mutated in individuals with ataxia
with oculomotor apraxia; relative distribution to nuclear
foci decreases upon DNA replication stress"
/codon_start=1
/product="DNA 5'-adenosine monophosphate hydrolase"
/protein_id="NP_014901.1"
/db_xref="GeneID:854432"
/db_xref="SGD:S000005784"
/translation="MSWRYALKNYVTSPETVNDDTVTYFDDKVSIIRDSFPKSECHLL
ILPRTMQLSRSHPTKVIDAKFKNEFESYVNSAIDHIFRHFQEKFRIKKSDDDKDPCWD
DILKDKNKFVRNFVQVGIHSVPSMANLHIHVISKDFHSVRLKNKKHYNSFNTGFFISW
DDLPLNGKNLGTDKEIETTYLKEHDLLCCYCQRNFSNKFSLLKKHLELEFNSHFELK"
gene complement(<812395..>813708)
/gene="RPT4"
/locus_tag="YOR259C"
/gene_synonym="CRL13; PCS1; SUG2"
/db_xref="GeneID:854433"
mRNA complement(<812395..>813708)
/gene="RPT4"
/locus_tag="YOR259C"
/gene_synonym="CRL13; PCS1; SUG2"
/product="proteasome regulatory particle base subunit
RPT4"
/transcript_id="NM_001183678.3"
/db_xref="GeneID:854433"
CDS complement(812395..813708)
/gene="RPT4"
/locus_tag="YOR259C"
/gene_synonym="CRL13; PCS1; SUG2"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10419517]"
/experiment="EXISTENCE:direct assay:GO:0008540 proteasome
regulatory particle, base subcomplex
[PMID:11742986|PMID:9741626]"
/experiment="EXISTENCE:direct assay:GO:0019904 protein
domain specific binding [PMID:11418596]"
/experiment="EXISTENCE:genetic interaction:GO:0006289
nucleotide-excision repair [PMID:11410533]"
/experiment="EXISTENCE:mutant phenotype:GO:0032968
positive regulation of transcription elongation by RNA
polymerase II [PMID:11389845]"
/experiment="EXISTENCE:mutant phenotype:GO:0036503 ERAD
pathway [PMID:18174173]"
/experiment="EXISTENCE:mutant phenotype:GO:0045899
positive regulation of RNA polymerase II transcription
preinitiation complex assembly [PMID:19843524]"
/experiment="EXISTENCE:mutant phenotype:GO:0070682
proteasome regulatory particle assembly [PMID:19412160]"
/experiment="EXISTENCE:physical interaction:GO:0031625
ubiquitin protein ligase binding [PMID:12447385]"
/note="ATPase of the 19S regulatory particle of the 26S
proteasome; one of six ATPases of the regulatory particle;
involved in degradation of ubiquitinated substrates;
contributes preferentially to ERAD; required for spindle
pole body duplication; mainly nuclear localization"
/codon_start=1
/product="proteasome regulatory particle base subunit
RPT4"
/protein_id="NP_014902.3"
/db_xref="GeneID:854433"
/db_xref="SGD:S000005785"
/translation="MSEEQDPLLAGLGETSGDNHTQQSHEQQPEQPQETEEHHEEEPS
RVDPEQEAHNKALNQFKRKLLEHRRYDDQLKQRRQNIRDLEKLYDKTENDIKALQSIG
QLIGEVMKELSEEKYIVKASSGPRYIVGVRNSVDRSKLKKGVRVTLDITTLTIMRILP
RETDPLVYNMTSFEQGEITFDGIGGLTEQIRELREVIELPLKNPEIFQRVGIKPPKGV
LLYGPPGTGKTLLAKAVAATIGANFIFSPASGIVDKYIGESARIIREMFAYAKEHEPC
IIFMDEVDAIGGRRFSEGTSADREIQRTLMELLTQMDGFDNLGQTKIIMATNRPDTLD
PALLRPGRLDRKVEIPLPNEAGRLEIFKIHTAKVKKTGEFDFEAAVKMSDGFNGADIR
NCATEAGFFAIRDDRDHINPDDLMKAVRKVAEVKKLEGTIEYQKL"
gene <813984..>815720
/gene="GCD1"
/locus_tag="YOR260W"
/gene_synonym="TRA3"
/db_xref="GeneID:854434"
mRNA <813984..>815720
/gene="GCD1"
/locus_tag="YOR260W"
/gene_synonym="TRA3"
/product="translation initiation factor eIF2B subunit
gamma"
/transcript_id="NM_001183679.1"
/db_xref="GeneID:854434"
CDS 813984..815720
/gene="GCD1"
/locus_tag="YOR260W"
/gene_synonym="TRA3"
/experiment="EXISTENCE:direct assay:GO:0005085
guanyl-nucleotide exchange factor activity
[PMID:9472020|PMID:8506384]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005851 eukaryotic
translation initiation factor 2B complex [PMID:8506384]"
/experiment="EXISTENCE:direct assay:GO:0006446 regulation
of translational initiation [PMID:8506384]"
/experiment="EXISTENCE:direct assay:GO:0032045
guanyl-nucleotide exchange factor complex
[PMID:8506384|PMID:9472020]"
/experiment="EXISTENCE:genetic interaction:GO:0006446
regulation of translational initiation [PMID:3516411]"
/experiment="EXISTENCE:mutant phenotype:GO:0003743
translation initiation factor activity
[PMID:2038327|PMID:11707417]"
/experiment="EXISTENCE:mutant phenotype:GO:0006446
regulation of translational initiation
[PMID:2038327|PMID:3516411|PMID:3915540|PMID:1986242]"
/experiment="EXISTENCE:mutant phenotype:GO:1903574
negative regulation of cellular response to amino acid
starvation [PMID:6351059]"
/experiment="EXISTENCE:physical interaction:GO:0003743
translation initiation factor activity [PMID:2038327]"
/experiment="EXISTENCE:physical interaction:GO:0005085
guanyl-nucleotide exchange factor activity [PMID:9472020]"
/experiment="EXISTENCE:physical interaction:GO:0032045
guanyl-nucleotide exchange factor complex [PMID:9472020]"
/note="Gamma subunit of the translation initiation factor
eIF2B; the guanine-nucleotide exchange factor for eIF2;
activity subsequently regulated by phosphorylated eIF2;
first identified as a negative regulator of GCN4
expression"
/codon_start=1
/product="translation initiation factor eIF2B subunit
gamma"
/protein_id="NP_014903.1"
/db_xref="GeneID:854434"
/db_xref="SGD:S000005786"
/translation="MSIQAFVFCGKGSNLAPFTQPDFPFQTQNKDSTAATSGDKLNEL
VNSALDSTVINEFMQHSTRLPKALLPIGNRPMIEYVLDWCDQADFKEISVVAPVDEIE
LIESGLTSFLSLRKQQFELIYKALSNSNHSHHLQDPKKINFIPSKANSTGESLQKELL
PRINGDFVILPCDFVTDIPPQVLVDQFRNRDDNNLAMTIYYKNSLDSSIDKKQQQKQK
QQQFFTVYSENEDSERQPILLDVYSQRDVTKTKYLQIRSHLLWNYPNLTVSTKLLNSF
IYFCSFELCQLLKLGPQSMSRQASFKDPFTGNQQQQNPPTTDDDEDRNHDDDDDYKPS
ATSIQPTYFKKKNDLILDPINCNKSLSKVFRDLSRRSWQHSKPREPIGIFILPNETLF
IRANNLNAYMDANRFVLKIKSQTMFTKNIQIQSAAIGADAIVDPKCQISAHSNVKMSV
LGTQANIGSRCRVAGSLLFPGVHLGDEVILENCIIGPMAKIGSKCKLSNCYIEGHYVV
EPKNNFKGETLANVYLDEDEEDELIYDDSVIAGESEIAEETDSDDRSDEDSDDSEYTD
EYEYEDDGLFER"
gene complement(<815915..>816931)
/gene="RPN8"
/locus_tag="YOR261C"
/db_xref="GeneID:854435"
mRNA complement(<815915..>816931)
/gene="RPN8"
/locus_tag="YOR261C"
/product="proteasome regulatory particle lid subunit RPN8"
/transcript_id="NM_001183680.3"
/db_xref="GeneID:854435"
CDS complement(815915..816931)
/gene="RPN8"
/locus_tag="YOR261C"
/experiment="EXISTENCE:direct assay:GO:0008541 proteasome
regulatory particle, lid subcomplex
[PMID:11742986|PMID:9741626]"
/experiment="EXISTENCE:direct assay:GO:0034515 proteasome
storage granule [PMID:18504300]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:27053109]"
/note="Essential non-ATPase regulatory subunit of the 26S
proteasome; has similarity to the human p40 proteasomal
subunit and to another S. cerevisiae regulatory subunit,
Rpn11p"
/codon_start=1
/product="proteasome regulatory particle lid subunit RPN8"
/protein_id="NP_014904.3"
/db_xref="GeneID:854435"
/db_xref="SGD:S000005787"
/translation="MSLQHEKVTIAPLVLLSALDHYERTQTKENKRCVGVILGDANSS
TIRVTNSFALPFEEDEKNSDVWFLDHNYIENMNEMCKKINAKEKLIGWYHSGPKLRAS
DLKINELFKKYTQNNPLLLIVDVKQQGVGLPTDAYVAIEQVKDDGTSTEKTFLHLPCT
IEAEEAEEIGVEHLLRDVRDQAAGGLSIRLTNQLKSLKGLQSKLKDVVEYLDKVINKE
LPINHTILGKLQDVFNLLPNLGTPDDDEIDVENHDRINISNNLQKALTVKTNDELMVI
YISNLVRSIIAFDDLIENKIQNKKIQEQRVKDKQSKVSDDSESESGDKEATAPLIQRK
NKKN"
gene <817292..>818335
/gene="GPN2"
/locus_tag="YOR262W"
/db_xref="GeneID:854436"
mRNA <817292..>818335
/gene="GPN2"
/locus_tag="YOR262W"
/product="GTPase GPN2"
/transcript_id="NM_001183681.1"
/db_xref="GeneID:854436"
CDS 817292..818335
/gene="GPN2"
/locus_tag="YOR262W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:14690591]"
/experiment="EXISTENCE:mutant phenotype:GO:0006606 protein
import into nucleus [PMID:23267056]"
/experiment="EXISTENCE:mutant phenotype:GO:0034087
establishment of mitotic sister chromatid cohesion
[PMID:18439903]"
/note="Putative GTPase with a specific role in RNA pol II
and polIII biogenesis; involved in the assembly and
subsequent nuclear import of RNA polymerases II and III;
required for establishment of sister chromatid cohesion;
contains a Gly-Pro-Asn motif in the G domain; similar to
Npa3p and Gpn3p; highly conserved across species and
homologous to human gene GPN2/ATPBD1B"
/codon_start=1
/product="GTPase GPN2"
/protein_id="NP_014905.1"
/db_xref="GeneID:854436"
/db_xref="SGD:S000005788"
/translation="MPFAQIVIGPPGSGKSTYCNGCSQFFNAIGRHSQVVNMDPANDA
LPYPCAVDIRDFITLEEIMQEQQLGPNGGLMYAVESLDNSIDLFILQIKSLVEEEKAY
LVFDCPGQVELFTHHSSLFNIFKKMEKELDIRFCVVNLIDCFYMTSPSQYISILLLAL
RSMLMMDLPHINVFSKIDMLKSYGELPFRLDYYTEVQDLDYLEPYIEKEGSSVLGKKY
SKLTETIKELVSDFNLVSFEVLSVDDKESMINLQGVIDKANGYIFGASEVGGDTVWAE
ASREGALIANYDIQDRWIDNKEKYDKEEEEKRTALLKEQELQNKAVDVNEEDEWENAL
KEWEEKQGMDFVR"
gene <818866..>820158
/gene="DSE3"
/locus_tag="YOR264W"
/db_xref="GeneID:854437"
mRNA <818866..>820158
/gene="DSE3"
/locus_tag="YOR264W"
/product="Dse3p"
/transcript_id="NM_001183683.3"
/db_xref="GeneID:854437"
CDS 818866..820158
/gene="DSE3"
/locus_tag="YOR264W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:14562095|PMID:22842922]"
/note="Daughter cell-specific protein, may help establish
daughter fate; relocalizes from bud neck to cytoplasm upon
DNA replication stress; interacts with Csm1p"
/codon_start=1
/product="Dse3p"
/protein_id="NP_014907.3"
/db_xref="GeneID:854437"
/db_xref="SGD:S000005790"
/translation="MPRKFLGNKIEKNVDAVRPSSLTLTADDLKYIPPIPQDFEDEDD
KVLRTSNGGNRLSKRFGGTLKLKKRLESVPELFLHDFKKRPRSQLEVIREKKFTDMQV
PKGPVCPQSTILPLRERKKVKSLPIQRKSLRRPTLSKPAVVQSLGHKTHSDHIIDKVF
VSRPAPIVMPVKALTPINPVSLMQTQTQDCCRKNKYGKSGSEILFDEILSAYENVSTS
DSTALNSEIDRIIDICASKQIAKKNEAFQVPYVVCPDDTETLFSSTTPKLKPVNSNTL
NDVISSPEYTTSGCSTYSDQSNSDEELSEVESIVWNTNKRTMRSSIVSESTSEEGYCT
AAETLPSTVSVEDLDIHNKLPKVAQTSSCNTLLNKLSIRKLKKVILDPPKIMHVMTFD
DDSDDGDDNDDEDRALNILQKKIDCIEIASCSSSIYSE"
gene <820454..>820774
/gene="RBL2"
/locus_tag="YOR265W"
/db_xref="GeneID:854439"
mRNA <820454..>820774
/gene="RBL2"
/locus_tag="YOR265W"
/product="Rbl2p"
/transcript_id="NM_001183684.1"
/db_xref="GeneID:854439"
CDS 820454..820774
/gene="RBL2"
/locus_tag="YOR265W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0015631 tubulin
binding [PMID:11739729]"
/experiment="EXISTENCE:mutant phenotype:GO:0007021 tubulin
complex assembly [PMID:11739729|PMID:10978276]"
/experiment="EXISTENCE:mutant phenotype:GO:0048487
beta-tubulin binding [PMID:11739729]"
/note="Protein involved in microtubule morphogenesis;
required for protection from excess free beta-tubulin;
proposed to be involved the folding of beta-tubulin;
similar to mouse beta-tubulin cofactor A; protein
abundance increases in response to DNA replication stress"
/codon_start=1
/product="Rbl2p"
/protein_id="NP_014908.1"
/db_xref="GeneID:854439"
/db_xref="SGD:S000005791"
/translation="MAPTQLDIKVKALKRLTKEEGYYQQELKDQEAHVAKLKEDKSVD
PYDLKKQEEVLDDTKRLLPTLYEKIREFKEDLEQFLKTYQGTEDVSDARSAITSAQEL
LDSK"
gene <821023..>822294
/gene="PNT1"
/locus_tag="YOR266W"
/db_xref="GeneID:854440"
mRNA <821023..>822294
/gene="PNT1"
/locus_tag="YOR266W"
/product="Pnt1p"
/transcript_id="NM_001183685.3"
/db_xref="GeneID:854440"
CDS 821023..822294
/gene="PNT1"
/locus_tag="YOR266W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:10490599]"
/experiment="EXISTENCE:genetic interaction:GO:0032979
protein insertion into mitochondrial inner membrane from
matrix [PMID:11950926]"
/experiment="EXISTENCE:mutant phenotype:GO:0032979 protein
insertion into mitochondrial inner membrane from matrix
[PMID:10490599]"
/experiment="EXISTENCE:physical interaction:GO:0032979
protein insertion into mitochondrial inner membrane from
matrix [PMID:11950926]"
/note="Mitochondrial integral inner membrane protein;
involved in membrane insertion of C-terminus of Cox2p,
interacts genetically and physically with Cox18p; deletion
mutant sensitive to the anti-Pneumocystis carinii drug
pentamidine"
/codon_start=1
/product="Pnt1p"
/protein_id="NP_014909.3"
/db_xref="GeneID:854440"
/db_xref="SGD:S000005792"
/translation="MDSRVALVRKYIAPSVIKSDSIQLHGLVKAPLFKALNSRYKLGS
LQIVQDVDWNAKTTPSDSPEPLAATLNSNRSLPMTKFPKQEILEQVKLDTKVGKWRKF
MTGWFRIGLYLLKSYKTGIQNTLKVFWDTRNEEQKFSIKNGALANLVREIEMHEINTR
LSSSSLPTSSSAKAPLRPLSINRKTLVELIRRDQIWKLPVFFTLVFIFEEVSVLIFTF
FPRVCPYNCLTPGGYKKLSNSYIKGTTSTQGNYGLGPLEFTKQGTIKYEPPYAVPIEN
LYNFLTSFPQSMISNWKLYIYKKLKLQKLLCNEIEKIYQYLFIDDWLLLQSILNTDVE
KTKIALSDRELVNCILERKLYHMGDDLNEMVNDTLGKEILLKRLFLYWTLRYNDTISL
NGKHTFSEKWGVNNISLLKYNSELVATKDIQ"
gene complement(<822588..>824867)
/gene="HRK1"
/locus_tag="YOR267C"
/db_xref="GeneID:854441"
mRNA complement(<822588..>824867)
/gene="HRK1"
/locus_tag="YOR267C"
/product="putative serine/threonine protein kinase HRK1"
/transcript_id="NM_001183686.1"
/db_xref="GeneID:854441"
CDS complement(822588..824867)
/gene="HRK1"
/locus_tag="YOR267C"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0006873
intracellular monoatomic ion homeostasis [PMID:11003661]"
/note="Protein kinase; implicated in activation of the
plasma membrane H(+)-ATPase Pma1p in response to glucose
metabolism and under acetic acid stress; plays a role in
ion homeostasis; maintains pH homeostasis and modulates
plasma membrane potential under acetic acid stress;
protein abundance increases in response to DNA replication
stress"
/codon_start=1
/product="putative serine/threonine protein kinase HRK1"
/protein_id="NP_014910.1"
/db_xref="GeneID:854441"
/db_xref="SGD:S000005793"
/translation="MPNLLSRNPFHGHHNDHHHDRENSSNNPPQLIRSSKSFLNFIGR
KQSNDSLRSEKSTDSMKSTTTTTNYTTTNLNNNTHSHSNATSISTNNYNNNYETNHHH
NISHGLHDYTSPASPKQTHSMAELKRFFRPSVNKKLSMSQLRSKKHSTHSPPPSKSTS
TVNLNNHYRAQHPHGFTDHYAHTQSAIPPSTDSILSLSNNINIYHDDCILAQKYGKLG
KLLGSGAGGSVKVLVRPTDGATFAVKEFRPRKPNESVKEYAKKCTAEFCIGSTLHHPN
VIETVDVFSDSKQNKYYEVMEYCPIDFFAVVMTGKMSRGEINCCLKQLTEGVKYLHSM
GLAHRDLKLDNCVMTSQGILKLIDFGSAVVFRYPFEDGVTMAHGIVGSDPYLAPEVIT
STKSYDPQCVDIWSIGIIYCCMVLKRFPWKAPRDSDDNFRLYCMPDDIEHDYVESARH
HEELLKERKEKRQRFLNHSDCSAINQQQPAHESNLKTVQNQVPNTPASIQGKSDNKPD
IVEEETEENKEDDSNNDKESTPDNDKESTIDIKISKNENKSTVVSANPKKVDADADAD
CDANGDSNGRVDCKANSDCNDKTDCNANNDCSNESDCNAKVDTNVNTAANANPDMVPQ
NNPQQQQQQQQQQQQQQQQQQQQHHHHQHQNQDKAHSIASDNKSSQQHRGPHHKKIIH
GPYRLLRLLPHASRPIMSRILQVDPKKRATLDDIFNDEWFAAIAACTMDSKNKVIRAP
GHHHTLVREENAHLETYKV"
gene complement(<825534..>825932)
/locus_tag="YOR268C"
/db_xref="GeneID:854442"
mRNA complement(<825534..>825932)
/locus_tag="YOR268C"
/product="uncharacterized protein"
/transcript_id="NM_001183687.3"
/db_xref="GeneID:854442"
CDS complement(825534..825932)
/locus_tag="YOR268C"
/note="hypothetical protein; sporulation is abnormal in
homozygous diploid; SWAT-GFP fusion protein localizes to
the nucleus; YOR268C is not an essential gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014911.3"
/db_xref="GeneID:854442"
/db_xref="SGD:S000005794"
/translation="MVFVFPFPFFSYGFSSFLEAGKKASYKMYYAEPELKTTRTGRAV
ACDAGSPRIIRVTLKDKIGLSERFTGRVFCYLAVACAWLSQYYHHTCAFFILYVHVCV
CFLFFRWCLFATVSLIHESQTQAGSAYYTK"
gene <826385..>827869
/gene="PAC1"
/locus_tag="YOR269W"
/gene_synonym="LIS1"
/db_xref="GeneID:854443"
mRNA <826385..>827869
/gene="PAC1"
/locus_tag="YOR269W"
/gene_synonym="LIS1"
/product="Pac1p"
/transcript_id="NM_001183688.1"
/db_xref="GeneID:854443"
CDS 826385..827869
/gene="PAC1"
/locus_tag="YOR269W"
/gene_synonym="LIS1"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:15965467]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005881 cytoplasmic
microtubule [PMID:15965467|PMID:12566428]"
/experiment="EXISTENCE:direct assay:GO:0051010 microtubule
plus-end binding [PMID:15965467]"
/experiment="EXISTENCE:mutant phenotype:GO:0030473 nuclear
migration along microtubule [PMID:12566428]"
/note="Regulator of dynein, involved in intracellular
transport; part of the dynein/dynactin pathway; targets
dynein to microtubule tips, which is necessary for sliding
of microtubules along bud cortex; serves at interface
between dynein's ATPase site and its microtubule binding
stalk, causing individual dynein motors to remain attached
to microtubules for long periods; synthetic lethal with
bni1; homolog of human LIS1, mutations in which cause the
severe brain disorder lissencephaly"
/codon_start=1
/product="Pac1p"
/protein_id="NP_014912.1"
/db_xref="GeneID:854443"
/db_xref="SGD:S000005795"
/translation="MTNWQQQLPLTDTQKNELDKSVLRYLNWNYKQTVRHEHAQDYES
VRHAIVTLSGFLLQESVDRQEFISNNDTSNESMVDIDELLLPKKWNSIVRLQKKIIEL
EQNTETLVSQIKDLNTQVSELAQFKPTTSNGTSAHNVLKWIPRNLPSCLINVESSVTS
VKLHPNLPIVFVATDHGKLYAFDLFNYTIPLASLQSHTKAITSMDVLFTNYTNSSKKN
YLVIVTASKDLQIHVFKWVSEECKFQQIRSLLGHEHIVSAVKIWQKNNDVHIASCSRD
QTVKIWDFHNGWSLKTFQPHSQWVRSIDVLGDYIISGSHDTTLRLTHWPSGNGLSVGT
GHEFPIEKVKFIHFIEDSPEIRFRTPSTDRYKNWGMQYCVSASRDRTIKIWEIPLPTL
MAHRAPIPNPTDSNFRCVLTLKGHLSWVRDISIRGQYLFSCADDKSVRCWDLNTGQCL
HVWEKLHTGFVNCLDLDVDFDSNVTPRQMMVTGGLDCKSNVFMR"
gene complement(<828052..>830574)
/gene="VPH1"
/locus_tag="YOR270C"
/db_xref="GeneID:854444"
mRNA complement(<828052..>830574)
/gene="VPH1"
/locus_tag="YOR270C"
/product="H(+)-transporting V0 sector ATPase subunit a"
/transcript_id="NM_001183689.3"
/db_xref="GeneID:854444"
CDS complement(828052..830574)
/gene="VPH1"
/locus_tag="YOR270C"
/experiment="EXISTENCE:direct assay:GO:0000220 vacuolar
proton-transporting V-type ATPase, V0 domain
[PMID:1385813]"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:11853670]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane
[PMID:23836928|PMID:27881666|PMID:22842922|PMID:18048916]"
/experiment="EXISTENCE:direct assay:GO:0045121 membrane
raft [PMID:23836928]"
/experiment="EXISTENCE:direct assay:GO:0046961
proton-transporting ATPase activity, rotational mechanism
[PMID:1385813|PMID:8798414]"
/experiment="EXISTENCE:direct assay:GO:0080025
phosphatidylinositol-3,5-bisphosphate binding
[PMID:31023825]"
/experiment="EXISTENCE:genetic interaction:GO:0071474
cellular hyperosmotic response [PMID:31023825]"
/experiment="EXISTENCE:mutant phenotype:GO:0006797
polyphosphate metabolic process [PMID:9933515]"
/experiment="EXISTENCE:mutant phenotype:GO:0007035
vacuolar acidification [PMID:1385813]"
/experiment="EXISTENCE:mutant phenotype:GO:0065003
protein-containing complex assembly
[PMID:8798414|PMID:1385813]"
/experiment="EXISTENCE:mutant phenotype:GO:1903778 protein
localization to vacuolar membrane [PMID:34342352]"
/note="Subunit a of the vacuolar-ATPase V0 domain; encodes
one of two isoforms, located in vacuolar V-ATPase
complexes while STV1 encodes the second isoform and is
located in Golgi and endosomal V-ATPase complexes;
interaction with PI(3,5)P2 increases V-ATPase activity and
contributes to hyperosmotic stress tolerance; relative
distribution to the vacuolar membrane decreases upon DNA
replication stress; human homolog ATP6V0A4 implicated in
renal tubular acidosis, can complement yeast null mutant"
/codon_start=1
/product="H(+)-transporting V0 sector ATPase subunit a"
/protein_id="NP_014913.3"
/db_xref="GeneID:854444"
/db_xref="SGD:S000005796"
/translation="MAEKEEAIFRSAEMALVQFYIPQEISRDSAYTLGQLGLVQFRDL
NSKVRAFQRTFVNEIRRLDNVERQYRYFYSLLKKHDIKLYEGDTDKYLDGSGELYVPP
SGSVIDDYVRNASYLEERLIQMEDATDQIEVQKNDLEQYRFILQSGDEFFLKGDNTDS
TSYMDEDMIDANGENIAAAIGASVNYVTGVIARDKVATLEQILWRVLRGNLFFKTVEI
EQPVYDVKTREYKHKNAFIVFSHGDLIIKRIRKIAESLDANLYDVDSSNEGRSQQLAK
VNKNLSDLYTVLKTTSTTLESELYAIAKELDSWFQDVTREKAIFEILNKSNYDTNRKI
LIAEGWIPRDELATLQARLGEMIARLGIDVPSIIQVLDTNHTPPTFHRTNKFTAGFQS
ICDCYGIAQYREINAGLPTIVTFPFMFAIMFGDMGHGFLMTLAALSLVLNEKKINKMK
RGEIFDMAFTGRYIILLMGVFSMYTGFLYNDIFSKTMTIFKSGWKWPDHWKKGESITA
TSVGTYPIGLDWAWHGTENALLFSNSYKMKLSILMGFIHMTYSYFFSLANHLYFNSMI
DIIGNFIPGLLFMQGIFGYLSVCIVYKWAVDWVKDGKPAPGLLNMLINMFLSPGTIDD
ELYPHQAKVQVFLLLMALVCIPWLLLVKPLHFKFTHKKKSHEPLPSTEADASSEDLEA
QQLISAMDADDAEEEEVGSGSHGEDFGDIMIHQVIHTIEFCLNCVSHTASYLRLWALS
LAHAQLSSVLWTMTIQIAFGFRGFVGVFMTVALFAMWFALTCAVLVLMEGTSAMLHSL
RLHWVESMSKFFVGEGLPYEPFAFEYKDMEVAVASASSSASS"
gene complement(<831059..>832042)
/gene="FSF1"
/locus_tag="YOR271C"
/db_xref="GeneID:854445"
mRNA complement(<831059..>832042)
/gene="FSF1"
/locus_tag="YOR271C"
/product="Fsf1p"
/transcript_id="NM_001183690.1"
/db_xref="GeneID:854445"
CDS complement(831059..832042)
/gene="FSF1"
/locus_tag="YOR271C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:24769239|PMID:16823961|PMID:26928762|PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0071454
cellular response to anoxia [PMID:23467670]"
/note="Putative protein; predicted to be an
alpha-isopropylmalate carrier; belongs to the
sideroblastic-associated protein family; non-tagged
protein is detected in purified mitochondria; likely to
play a role in iron homeostasis"
/codon_start=1
/product="Fsf1p"
/protein_id="NP_014914.1"
/db_xref="GeneID:854445"
/db_xref="SGD:S000005797"
/translation="MASSVPGPIDLPESRYDLSTYWGRIRHCAEISDPTMLLTTEKDL
AHAREIISAYRHGELKETTPEFWRAKKQLDSTVHPDTGKTVLLPFRMSSNVLSNLVVT
VGMLTPGLGTAGTVFWQWANQSLNVAVNSANANKSHPMSTSQLLTNYAAAVTASCGVA
LGLNNLVPRLKNISPHSKLILGRLVPFAAVVSAGIVNVFLMRGNEIRKGISVFDSNGD
EVGKSKKAAFMAVGETALSRVINATPTMVIPPLILVRLQRGVLKGKSLGVQTLANLGL
ISVTMFSALPFALGIFPQRQAIHLNKLEPELHGKKDKDGKPIEKVYFNRGI"
gene 832332..832521
/gene="SNR8"
/locus_tag="YNCO0026W"
/db_xref="GeneID:9164971"
ncRNA 832332..832521
/ncRNA_class="snoRNA"
/gene="SNR8"
/locus_tag="YNCO0026W"
/product="SNR8"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:9160748]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:3327689]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:3327689]"
/experiment="EXISTENCE:mutant phenotype:GO:0030559 rRNA
pseudouridylation guide activity [PMID:9160748]"
/experiment="EXISTENCE:mutant phenotype:GO:0031118 rRNA
pseudouridine synthesis [PMID:9160748]"
/experiment="EXISTENCE:physical interaction:GO:0003723 RNA
binding [PMID:3327689]"
/experiment="EXISTENCE:physical interaction:GO:0031429 box
H/ACA snoRNP complex [PMID:15923376]"
/note="H/ACA box small nucleolar RNA (snoRNA); guides
pseudouridylation of large subunit (LSU) rRNA at positions
U960 and U986"
/transcript_id="NR_132263.1"
/db_xref="GeneID:9164971"
/db_xref="SGD:S000006497"
gene <832813..>834195
/gene="YTM1"
/locus_tag="YOR272W"
/gene_synonym="CST14"
/db_xref="GeneID:854446"
mRNA <832813..>834195
/gene="YTM1"
/locus_tag="YOR272W"
/gene_synonym="CST14"
/product="Ytm1p"
/transcript_id="NM_001183691.3"
/db_xref="GeneID:854446"
CDS 832813..834195
/gene="YTM1"
/locus_tag="YOR272W"
/gene_synonym="CST14"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:11583614]"
/experiment="EXISTENCE:direct assay:GO:0030687
preribosome, large subunit precursor
[PMID:11583614|PMID:17443350]"
/experiment="EXISTENCE:direct assay:GO:0070545 PeBoW
complex [PMID:16287855]"
/experiment="EXISTENCE:direct assay:GO:0110136 protein-RNA
complex remodeling [PMID:20542003]"
/experiment="EXISTENCE:mutant phenotype:GO:0042273
ribosomal large subunit biogenesis [PMID:11583614]"
/experiment="EXISTENCE:mutant phenotype:GO:0051276
chromosome organization [PMID:10454593]"
/experiment="EXISTENCE:physical interaction:GO:0070545
PeBoW complex [PMID:16287855]"
/note="Ribosomal assembly factor and 66S pre-ribosomal
particle constituent; subunit of the Nop7-subcomplex
(PeBoW complex), required for an early nucleolar step in
pre-60S ribosomal particle maturation; interaction of its
ubiquitin-like (UBL) domain with the MIDAS domain in the
Rea1p tail triggers release of the subcomplex and possibly
other biogenesis factors via cycles of ATP hydrolysis;
involved in the processing of 27S pre-rRNA; contains an
N-terminal UBL domain and seven C-terminal WD repeats"
/codon_start=1
/product="Ytm1p"
/protein_id="NP_014915.3"
/db_xref="GeneID:854446"
/db_xref="SGD:S000005798"
/translation="MTEDKSQVKIRFFTREKDELLHVQDTPMYAPISLKRYGLSEIVN
HLLGSEKPVPFDFLIEGELLRTSLHDYLTKKGLSSEASLNVEYTRAILPPSYLNSFSN
EDWVSSLDVGDGSKHIISGSYDGIVRTWDLSGNVQKQYSGHSGPIRAVKYISNTRLVS
AGNDRTLRLWKTKNDDLKLTSQQQAQEDDDDEVNIEDGKTLAILEGHKAPVVSIDVSD
NSRILSASYDNSIGFWSTIYKEMTVVDPLEDINNPNNKISTAARKRRKLTMKDGTIRR
RAPLSLLESHTAPVEQVIFDSTDNTVGYSVSQDHTIKTWDLVTARCIDTRTTSYSLLS
IAQLSTLNLLACGSSARHITLHDPRVGASSKVTQQQLIGHKNFVSSLDTCPENEYILC
SGSHDGTVKVWDVRSTSPMYTITREDKSVQKGVNDKVFAVKWAEKVGIISAGQDKKIQ
INKGDNIFKN"
gene complement(<834452..>836431)
/gene="TPO4"
/locus_tag="YOR273C"
/db_xref="GeneID:854447"
mRNA complement(<834452..>836431)
/gene="TPO4"
/locus_tag="YOR273C"
/product="Tpo4p"
/transcript_id="NM_001183692.1"
/db_xref="GeneID:854447"
CDS complement(834452..836431)
/gene="TPO4"
/locus_tag="YOR273C"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:12562762]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0000296
spermine transport [PMID:11171066]"
/experiment="EXISTENCE:mutant phenotype:GO:0000297
spermine transmembrane transporter activity
[PMID:11171066]"
/experiment="EXISTENCE:mutant phenotype:GO:0000329
fungal-type vacuole membrane [PMID:11171066]"
/experiment="EXISTENCE:mutant phenotype:GO:0015606
spermidine transmembrane transporter activity
[PMID:11171066]"
/experiment="EXISTENCE:mutant phenotype:GO:0015848
spermidine transport [PMID:11171066]"
/note="Polyamine transporter of the major facilitator
superfamily; member of the 12-spanner drug:H(+) antiporter
DHA1 family; recognizes spermine, putrescine, and
spermidine; localizes to the plasma membrane"
/codon_start=1
/product="Tpo4p"
/protein_id="NP_014916.1"
/db_xref="GeneID:854447"
/db_xref="SGD:S000005799"
/translation="MPSSLTKTESNSDPRTNIQQVPKALDKNVTNSGNLDSTSSSTGS
ITEDEKRSEPNADSNNMTGGEPIDPRDLDWDGPDDPDNPHNWSSLKKWYTTMTSAFLC
LVVTMGSSLYVSSVPELVERYHVSQTLALAGLTFYLLGLSTVIGAPLSEVFGRKPVYL
FSLPVSMLFTMGVGLSNGHMRIILPLRFLSGVFASPALSVGSGTILDIFDVDQVSVAM
TYFVLSPFLGPVLSPIMAGFATEAKGWRWSEWIQLIAGGLILPFIALMPETHKGIILR
KRAKKRNIALKKFSREAQKEFLKTTVTITILRPLKMLVVEPIVFVFSVYVAFIFAILF
GFFEAYAVIYRGVYHMSMGISGLPFIGIGVGLWIGAFFYLYIDRKYLFPKPPAGTQPL
TEKERTSKRTTPYRGARDAETGELLPVVPEKFLIACKFGSVALPIGLFWQAWTARSDV
HWMAPVAAGVPFGFGLILIFFSVLMYFSTCYPPLTVASCLAANNLLRYVMSSVFPLFT
IQMYTKMKIKWASTLFALVCVVMIPIPWVFEKWGSKLRHKSQFGYAAMEKEAETEGGI
DDVNAVDGELNLTRMTTLRTMETDPSTREKPGERLSLRRTHTQPVPASFDREDGQHAQ
NRNEPISNSLYSAIKDNEDGYSYTEMATDASARMV"
gene <837674..>838960
/gene="MOD5"
/locus_tag="YOR274W"
/db_xref="GeneID:854448"
mRNA <837674..>838960
/gene="MOD5"
/locus_tag="YOR274W"
/product="tRNA dimethylallyltransferase"
/transcript_id="NM_001183693.3"
/db_xref="GeneID:854448"
CDS 837674..838960
/gene="MOD5"
/locus_tag="YOR274W"
/EC_number="2.5.1.75"
/experiment="EXISTENCE:direct assay:GO:0000049 tRNA
binding [PMID:23898186]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9872948]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:9872948]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:9872948|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:9872948]"
/experiment="EXISTENCE:direct assay:GO:0006400 tRNA
modification [PMID:18852462]"
/experiment="EXISTENCE:direct assay:GO:0052381 tRNA
dimethylallyltransferase activity
[PMID:18852462|PMID:3031456]"
/experiment="EXISTENCE:mutant phenotype:GO:0006400 tRNA
modification [PMID:3031456]"
/note="Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl
transferase; required for biosynthesis of
isopentenyladenosine in mitochondrial and cytoplasmic
tRNAs; also has a role in tRNA gene-mediated silencing;
gene encodes two isozymic forms; converts to a prion form,
prion conversion contributes to azole antifungal
resistance by upregulating ergosterol biosynthesis;
homolog of human TRIT1, a mutation in which is associated
with severe combined respiratory chain defects"
/codon_start=1
/product="tRNA dimethylallyltransferase"
/protein_id="NP_014917.3"
/db_xref="GeneID:854448"
/db_xref="SGD:S000005800"
/translation="MLKGPLKGCLNMSKKVIVIAGTTGVGKSQLSIQLAQKFNGEVIN
SDSMQVYKDIPIITNKHPLQEREGIPHHVMNHVDWSEEYYSHRFETECMNAIEDIHRR
GKIPIVVGGTHYYLQTLFNKRVDTKSSERKLTRKQLDILESTDPDVIYNTLVKCDPDI
ATKYHPNDYRRVQRMLEIYYKTGKKPSETFNEQKITLKFDTLFLWLYSKPEPLFQRLD
DRVDDMLERGALQEIKQLYEYYSQNKFTPEQCENGVWQVIGFKEFLPWLTGKTDDNTV
KLEDCIERMKTRTRQYAKRQVKWIKKMLIPDIKGDIYLLDATDLSQWDTNASQRAIAI
SNDFISNRPIKQERAPKALEELLSKGETTMKKLDDWTHYTCNVCRNADGKNVVAIGEK
YWKIHLGSRRHKSNLKRNTRQADFEKWKINKKETVE"
gene complement(<839084..>841069)
/gene="RIM20"
/locus_tag="YOR275C"
/db_xref="GeneID:854449"
mRNA complement(<839084..>841069)
/gene="RIM20"
/locus_tag="YOR275C"
/product="Rim20p"
/transcript_id="NM_001183694.1"
/db_xref="GeneID:854449"
CDS complement(839084..841069)
/gene="RIM20"
/locus_tag="YOR275C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:36164978]"
/experiment="EXISTENCE:direct assay:GO:0016485 protein
processing [PMID:11698381]"
/experiment="EXISTENCE:mutant phenotype:GO:0001403
invasive growth in response to glucose limitation
[PMID:18202364|PMID:11698381]"
/experiment="EXISTENCE:mutant phenotype:GO:0016485 protein
processing [PMID:11698381]"
/experiment="EXISTENCE:mutant phenotype:GO:0030435
sporulation resulting in formation of a cellular spore
[PMID:11698381]"
/note="Protein involved in proteolytic activation of
Rim101p; part of response to alkaline pH; PalA/AIP1/Alix
family member; interaction with the ESCRT-III subunit
Snf7p suggests a relationship between pH response and
multivesicular body formation; localizes to cytosol,
nucleus and peroxisomes"
/codon_start=1
/product="Rim20p"
/protein_id="NP_014918.1"
/db_xref="GeneID:854449"
/db_xref="SGD:S000005801"
/translation="MSELLAIPLKRTLEVDFATELSKLIDTTSFQTASFFQSDILKVV
DARNNAIAPDISIDGLSALKEYYVILLQLEKKFPNNQIEFTWFQTLSQKSRGTSQYSL
QWEKLTIIYNIGCMYSLLALNSNNDAAESLKTSCLYFQNAAGCFKHVLDHQKNLETIP
VVDDATLNALTSLMLAQAQECFWFKAVQDKHKDSLIAKLSQQIVDFYCEAINDAQRGK
LIRSDWINHLKAKKAYFSAVTYYRIALSFNEKKQFGNVVKALQMGLQFINESTLSSQA
KFKTVVESSLKEAQRDNEFIYLQEVPSELPSIKPALMVKPSSSATLLPSIKKDETLFK
DLIPIEVMEYCTAYNERQDEYVEQRVTNPLASLNKLLKESLTTFQIPQGLTKVSEAEL
SHYQASLNNLLINNKNVQVQLDNIEQILNEEAFTDNQLRLKHGTLNWTLPESSTTNTA
YYEKLKKLRGYLDEGSAIDKQTNELFQSIDKNLIGSEIRLPESNDPLTNKIKMIIQER
NDYIDRTRRKSSEYRILPKIITSYKKNGTVDFEPIFIGHLKYFDEDLRYVNSTKEENI
KLIEEVNLSKKNNPGRSGIEPKKMVRIDPRELYIEDLRYSFKLLDEVKENLSAGTAFY
ENLITSTSNLYNEVQEYDTARRAEKARLDKSLTFEDQ"
gene <841333..>841818
/gene="CAF20"
/locus_tag="YOR276W"
/gene_synonym="CAF2; CAP20"
/db_xref="GeneID:854450"
mRNA <841333..>841818
/gene="CAF20"
/locus_tag="YOR276W"
/gene_synonym="CAF2; CAP20"
/product="Caf20p"
/transcript_id="NM_001183695.3"
/db_xref="GeneID:854450"
CDS 841333..841818
/gene="CAF20"
/locus_tag="YOR276W"
/gene_synonym="CAF2; CAP20"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008190 eukaryotic
initiation factor 4E binding [PMID:9707439]"
/experiment="EXISTENCE:direct assay:GO:0017148 negative
regulation of translation [PMID:9118949]"
/experiment="EXISTENCE:mutant phenotype:GO:0010606
positive regulation of cytoplasmic mRNA processing body
assembly [PMID:18182159]"
/experiment="EXISTENCE:mutant phenotype:GO:0017148
negative regulation of translation [PMID:9144215]"
/experiment="EXISTENCE:mutant phenotype:GO:0030447
filamentous growth [PMID:17041186]"
/experiment="EXISTENCE:mutant phenotype:GO:0045727
positive regulation of translation [PMID:17041186]"
/note="Phosphoprotein of the mRNA cap-binding complex;
involved in translational control; repressor of
cap-dependent translational initiation; translational
activator of select mRNAs during filamentous growth;
competes with eIF4G for binding to eIF4E"
/codon_start=1
/product="Caf20p"
/protein_id="NP_014919.3"
/db_xref="GeneID:854450"
/db_xref="SGD:S000005802"
/translation="MIKYTIDELFQLKPSLTLEVNFDAVEFRAIIEKVKQLQHLKEEE
FNSHHVGHFGRRRSSHHHGRPKIKHNKPKVTTDSDGWCTFEAKKKGSGEDDEEETETT
PTSTVPVATIAQETLKVKPNNKNISSNRPADTRDIVADKPILGFNAFAALESEDEDDE
A"
gene complement(841958..842182)
/gene="SNR31"
/locus_tag="YNCO0027C"
/db_xref="GeneID:9164979"
ncRNA complement(841958..842182)
/ncRNA_class="snoRNA"
/gene="SNR31"
/locus_tag="YNCO0027C"
/product="SNR31"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:9160748]"
/experiment="EXISTENCE:mutant phenotype:GO:0030559 rRNA
pseudouridylation guide activity [PMID:9160748]"
/experiment="EXISTENCE:mutant phenotype:GO:0031118 rRNA
pseudouridine synthesis [PMID:9160748]"
/experiment="EXISTENCE:physical interaction:GO:0031429 box
H/ACA snoRNP complex [PMID:15923376|PMID:9472021]"
/note="H/ACA box small nucleolar RNA (snoRNA); guides
pseudouridylation of small subunit (SSU) rRNA at position
U999"
/transcript_id="NR_132264.1"
/db_xref="GeneID:9164979"
/db_xref="SGD:S000007296"
gene 842403..842606
/gene="SNR5"
/locus_tag="YNCO0028W"
/db_xref="GeneID:9164980"
ncRNA 842403..842606
/ncRNA_class="snoRNA"
/gene="SNR5"
/locus_tag="YNCO0028W"
/product="SNR5"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:9182768]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:3327689]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:3327689]"
/experiment="EXISTENCE:mutant phenotype:GO:0030559 rRNA
pseudouridylation guide activity [PMID:9182768]"
/experiment="EXISTENCE:mutant phenotype:GO:0031118 rRNA
pseudouridine synthesis [PMID:9182768]"
/experiment="EXISTENCE:physical interaction:GO:0003723 RNA
binding [PMID:3327689]"
/experiment="EXISTENCE:physical interaction:GO:0031429 box
H/ACA snoRNP complex [PMID:15923376]"
/note="H/ACA box small nucleolar RNA (snoRNA); guides
pseudouridylation of large subunit (LSU) rRNA at positions
U1004 and U1124"
/transcript_id="NR_132265.1"
/db_xref="GeneID:9164980"
/db_xref="SGD:S000007291"
gene <842817..>843644
/gene="HEM4"
/locus_tag="YOR278W"
/gene_synonym="SLU2"
/db_xref="GeneID:854452"
mRNA <842817..>843644
/gene="HEM4"
/locus_tag="YOR278W"
/gene_synonym="SLU2"
/product="uroporphyrinogen-III synthase HEM4"
/transcript_id="NM_001183697.1"
/db_xref="GeneID:854452"
CDS 842817..843644
/gene="HEM4"
/locus_tag="YOR278W"
/gene_synonym="SLU2"
/EC_number="4.2.1.75"
/experiment="EXISTENCE:mutant phenotype:GO:0004852
uroporphyrinogen-III synthase activity [PMID:7597845]"
/experiment="EXISTENCE:mutant phenotype:GO:0006783 heme
biosynthetic process [PMID:18042043|PMID:14559249]"
/note="Uroporphyrinogen III synthase; catalyzes the
conversion of hydroxymethylbilane to uroporphyrinogen III,
the fourth step in heme biosynthesis; deficiency in the
human homolog can result in the disease congenital
erythropoietic porphyria"
/codon_start=1
/product="uroporphyrinogen-III synthase HEM4"
/protein_id="NP_014921.1"
/db_xref="GeneID:854452"
/db_xref="SGD:S000005804"
/translation="MSSRKKVRVLLLKNKTVPIDKYELECRSKAFEPIFVPLIKHTHV
IQDFRNVLNTIPNYLNTINYIIITSQRTVESLNEAIIPTLTSEQKAALLSKTVYTVGP
ATANFIRRSGFINVKGGEDAGNGSILADIIIDDLSTDIKACPPSELLFLVGEIRRDII
PKKLHSKGIKVREVVTYKTEELSDGFKRFIHAMKECDEDEVFSDWVVVFSPQGTKEIT
QYLGDSNRLPGSHLRVASIGPTTKKYLDDNDVTSDVVSPKPDPKSLLDAIELYQRHK"
gene complement(<843698..>844630)
/gene="RFM1"
/locus_tag="YOR279C"
/db_xref="GeneID:854453"
mRNA complement(<843698..>844630)
/gene="RFM1"
/locus_tag="YOR279C"
/product="Rfm1p"
/transcript_id="NM_001183698.3"
/db_xref="GeneID:854453"
CDS complement(843698..844630)
/gene="RFM1"
/locus_tag="YOR279C"
/experiment="EXISTENCE:genetic interaction:GO:0032298
positive regulation of DNA-templated DNA replication
initiation [PMID:18990212]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:12612074]"
/experiment="EXISTENCE:mutant phenotype:GO:0000278 mitotic
cell cycle [PMID:12612074]"
/experiment="EXISTENCE:physical interaction:GO:0000118
histone deacetylase complex [PMID:12612074]"
/experiment="EXISTENCE:physical interaction:GO:0030674
protein-macromolecule adaptor activity [PMID:12612074]"
/note="Component of the Sum1p-Rfm1p-Hst1p complex; Rfm1p
tethers the Hst1p histone deacetylase to the DNA-binding
protein Sum1p; complex is involved in transcriptional
repression of middle sporulation genes and in initiation
of DNA replication"
/codon_start=1
/product="Rfm1p"
/protein_id="NP_014922.3"
/db_xref="GeneID:854453"
/db_xref="SGD:S000005805"
/translation="MSTNTEIIKNSDLQSLINDKRRLINEIKDFNKSIKPLEFESYQD
YFLIKTFKKGISASGHVDIDSLRNKEYGIYYKKIKRNSTQEVGEPIPRNTSSSSSSTR
SNSSADISDTEYSGENTPTTTGAASRRRRTRSRAIQRENSLPASLPSISEANANNDDV
TISEINGSELPFPIPISEVENIDIASDITERDGIRRRSSRISERDKRRSQSRLGSEED
EEGDGHDGDEGETKIQDLYESLVPKILESRRRSDWILPPKARYTPEKQMRTKPSFKSI
KINELVGNKRIRSILSRFEGGVAGIRKRDWDSTQ"
gene complement(<844992..>845792)
/gene="FSH3"
/locus_tag="YOR280C"
/db_xref="GeneID:854454"
mRNA complement(<844992..>845792)
/gene="FSH3"
/locus_tag="YOR280C"
/product="putative serine hydrolase"
/transcript_id="NM_001183699.1"
/db_xref="GeneID:854454"
CDS complement(844992..845792)
/gene="FSH3"
/locus_tag="YOR280C"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:36164978]"
/note="Putative serine hydrolase; likely target of
Cyc8p-Tup1p-Rfx1p transcriptional regulation; localizes to
cytosol and peroxisomes; similar to S. cerevisiae Fsh1p
and Fsh2p and the human candidate tumor suppressor OVCA2"
/codon_start=1
/product="putative serine hydrolase"
/protein_id="NP_014923.1"
/db_xref="GeneID:854454"
/db_xref="SGD:S000005806"
/translation="MSEKKKVLMLHGFVQSDKIFSAKTGGLRKNLKKLGYDLYYPCAP
HSIDKKALFQSESEKGRDAAKEFNTSATSDEVYGWFFRNPESFNSFQIDQKVFNYLRN
YVLENGPFDGVIGFSQGAGLGGYLVTDFNRILNLTDEQQPALKFFISFSGFKLEDQSY
QKEYHRIIQVPSLHVRGELDEVVAESRIMALYESWPDNKRTLLVHPGAHFVPNSKPFV
SQVCNWIQGITSKEGQEHNAQPEVDRKQFDKPQLEDDLLDMIDSLGKL"
gene complement(<846269..>847129)
/gene="PLP2"
/locus_tag="YOR281C"
/db_xref="GeneID:854456"
mRNA complement(<846269..>847129)
/gene="PLP2"
/locus_tag="YOR281C"
/product="Plp2p"
/transcript_id="NM_001183700.3"
/db_xref="GeneID:854456"
CDS complement(846269..847129)
/gene="PLP2"
/locus_tag="YOR281C"
/experiment="EXISTENCE:direct assay:GO:0003779 actin
binding [PMID:19501098]"
/experiment="EXISTENCE:direct assay:GO:0006457 protein
folding [PMID:19501098]"
/experiment="EXISTENCE:direct assay:GO:0031683 G-protein
beta/gamma-subunit complex binding [PMID:10749875]"
/experiment="EXISTENCE:mutant phenotype:GO:0003779 actin
binding [PMID:19501098]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:10749875]"
/experiment="EXISTENCE:mutant phenotype:GO:0071444
cellular response to pheromone [PMID:10749875]"
/experiment="EXISTENCE:physical interaction:GO:0005737
cytoplasm [PMID:10749875]"
/note="Protein that interacts with the CCT complex to
stimulate actin folding; has similarity to phosducins;
null mutant lethality is complemented by mouse
phosducin-like protein MgcPhLP; CCT is short for
chaperonin containing TCP-1; essential gene"
/codon_start=1
/product="Plp2p"
/protein_id="NP_014924.3"
/db_xref="GeneID:854456"
/db_xref="SGD:S000005807"
/translation="MQNEPMFQVQVDESEDSEWNDILRAKGVIPERAPSPTAKLEEAL
EEAIAKQHENRLEDKDLSDLEELEDDEDEDFLEAYKIKRLNEIRKLQERSKFGEVFHI
NKPEYNKEVTLASQGKKYEGAQTNDNGEEDDGGVYVFVHLSLQSKLQSRILSHLFQSA
ACKFREIKFVEIPANRAIENYPESNCPTLIVYYRGEVIKNMITLLELGGNNSKMEDFE
DFMVKVGAVAEGDNRLIMNRDDEESREERKLHYGEKKSIRSGIRGKFNVGIGGNDDGN
INDDDDGFFD"
gene <847453..>848145
/locus_tag="YOR283W"
/db_xref="GeneID:854457"
mRNA <847453..>848145
/locus_tag="YOR283W"
/product="phosphoglycerate mutase"
/transcript_id="NM_001183702.1"
/db_xref="GeneID:854457"
CDS 847453..848145
/locus_tag="YOR283W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0016311
dephosphorylation [PMID:19753119]"
/experiment="EXISTENCE:direct assay:GO:0016791 phosphatase
activity [PMID:20427268|PMID:19753119]"
/note="Phosphatase with a broad substrate specificity; has
some similarity to GPM1/YKL152C, a phosphoglycerate
mutase; YOR283W is not an essential gene"
/codon_start=1
/product="phosphoglycerate mutase"
/protein_id="NP_014926.1"
/db_xref="GeneID:854457"
/db_xref="SGD:S000005809"
/translation="MTKEVPYYCDNDDNNIIRLFIIRHGQTEHNVKKILQGHKDTSIN
PTGEEQATKLGHYLRSRGIHFDKVVSSDLKRCRQTTALVLKHSKQENVPTSYTSGLRE
RYMGVIEGMQITEAEKYADKHGEGSFRNFGEKSDDFVARLTGCVEEEVAEASNEGVKN
LALVSHGGAIRMILQWLKYENHQAHKIIVFNTSVTIVDYVKDSKQFIVRRVGNTQHLG
DGEFVVSDLRLR"
gene <848478..>849209
/gene="HUA2"
/locus_tag="YOR284W"
/db_xref="GeneID:854458"
mRNA <848478..>849209
/gene="HUA2"
/locus_tag="YOR284W"
/product="Hua2p"
/transcript_id="NM_001183703.3"
/db_xref="GeneID:854458"
CDS 848478..849209
/gene="HUA2"
/locus_tag="YOR284W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="Cytoplasmic hypothetical protein; computational
analysis of large-scale protein-protein interaction data
suggests a possible role in actin patch assembly"
/codon_start=1
/product="Hua2p"
/protein_id="NP_014927.3"
/db_xref="GeneID:854458"
/db_xref="SGD:S000005810"
/translation="MQQTKFGKMYLDHDSVVEYSEDEIVEADRITLGYKKRLSMIENQ
MRHLLEDFSLDVQQIEPILADLQKYYDAFLQLLQKRNKSLQCKRSTHQPVPSPMNSQT
STNAKVNLSGKLMKFQLNSVQKFDEENILRILQNKIEFEHYFQIDKGKKQKVLLLAVY
QCLNGPTRLHKVLNIEGIIHNNSIRTILGKQVSSSKWTVFLYDVKLVLLAHRQDVPNL
ETSKMIVRYGDLFPCALYFKDHTAY"
gene <849635..>850054
/gene="RDL1"
/locus_tag="YOR285W"
/db_xref="GeneID:854459"
mRNA <849635..>850054
/gene="RDL1"
/locus_tag="YOR285W"
/product="thiosulfate sulfurtransferase RDL1"
/transcript_id="NM_001183704.1"
/db_xref="GeneID:854459"
CDS 849635..850054
/gene="RDL1"
/locus_tag="YOR285W"
/experiment="EXISTENCE:direct assay:GO:0004792
thiosulfate-cyanide sulfurtransferase activity
[PMID:24981631]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:16407407]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006790 sulfur
compound metabolic process [PMID:30217845]"
/note="Thiosulfate sulfurtransferase; contains a
rhodanese-like domain; localized to the mitochondrial
outer membrane; protein abundance increases in response to
DNA replication stress; similar to the human TSTD gene"
/codon_start=1
/product="thiosulfate sulfurtransferase RDL1"
/protein_id="NP_014928.1"
/db_xref="GeneID:854459"
/db_xref="SGD:S000005811"
/translation="MWKAVMNAWNGTESQSKNVSNIQSYSFEDMKRIVGKHDPNVVLV
DVREPSEYSIVHIPASINVPYRSHPDAFALDPLEFEKQIGIPKPDSAKELIFYCASGK
RGGEAQKVASSHGYSNTSLYPGSMNDWVSHGGDKLDL"
gene <850280..>850729
/gene="RDL2"
/locus_tag="YOR286W"
/gene_synonym="AIM42"
/db_xref="GeneID:854460"
mRNA <850280..>850729
/gene="RDL2"
/locus_tag="YOR286W"
/gene_synonym="AIM42"
/product="thiosulfate sulfurtransferase RDL2"
/transcript_id="NM_001183705.3"
/db_xref="GeneID:854460"
CDS 850280..850729
/gene="RDL2"
/locus_tag="YOR286W"
/gene_synonym="AIM42"
/EC_number="2.8.1.1"
/experiment="EXISTENCE:direct assay:GO:0004792
thiosulfate-cyanide sulfurtransferase activity
[PMID:19864628]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:16823961|PMID:24769239|PMID:14576278|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006790 sulfur
compound metabolic process [PMID:30217845]"
/note="Protein with rhodanese activity; contains a
rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and
Ych1p; overexpression causes a cell cycle delay; null
mutant displays elevated frequency of mitochondrial genome
loss"
/codon_start=1
/product="thiosulfate sulfurtransferase RDL2"
/protein_id="NP_014929.3"
/db_xref="GeneID:854460"
/db_xref="SGD:S000005812"
/translation="MFKHSTGILSRTVSARSPTLVLRTFTTKAPKIYTFDQVRNLVEH
PNDKKLLVDVREPKEVKDYKMPTTINIPVNSAPGALGLPEKEFHKVFQFAKPPHDKEL
IFLCAKGVRAKTAEELARSYGYENTGIYPGSITEWLAKGGADVKPKK"
gene complement(<850937..>851839)
/gene="RRP36"
/locus_tag="YOR287C"
/db_xref="GeneID:854461"
mRNA complement(<850937..>851839)
/gene="RRP36"
/locus_tag="YOR287C"
/product="rRNA-processing protein RRP36"
/transcript_id="NM_001183706.1"
/db_xref="GeneID:854461"
CDS complement(850937..851839)
/gene="RRP36"
/locus_tag="YOR287C"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:20038530]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:20038530]"
/experiment="EXISTENCE:mutant phenotype:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12837249]"
/experiment="EXISTENCE:mutant phenotype:GO:0006364 rRNA
processing [PMID:20038530]"
/experiment="EXISTENCE:mutant phenotype:GO:0042274
ribosomal small subunit biogenesis [PMID:20038530]"
/note="Component of 90S preribosomes; involved in early
cleavages of the 35S pre-rRNA and in production of the 40S
ribosomal subunit"
/codon_start=1
/product="rRNA-processing protein RRP36"
/protein_id="NP_014930.1"
/db_xref="GeneID:854461"
/db_xref="SGD:S000005813"
/translation="MSYYFKNLKPDLNSDVEEDDGNLLESIMANKSKREIDEQESSDD
ELKTLSFGSLKKAETVIDEEDFKDTKPVHKKPITTTYREESFDEDEDSEDQSDEDAGF
FEEDSEDETHHGQKVPKKKSKHAPVEQSSKKRVPRVRNIPGLEIPRNKRSNLYQDIRF
DKSTGKALDSSIIRKRYQFLDEYREKEIDELQKLLQERKFLSKIDQGEREEMEQRLKS
MKSRLQSMKNKDLEREILKEYENDMNKNNNTRYHLKKSEKRKVVQKWKFDHMKAKQRE
KVMERKRKKRLGKEFKQFEFHNRR"
gene complement(<852121..>853077)
/gene="MPD1"
/locus_tag="YOR288C"
/db_xref="GeneID:854462"
mRNA complement(<852121..>853077)
/gene="MPD1"
/locus_tag="YOR288C"
/product="protein disulfide isomerase MPD1"
/transcript_id="NM_001183707.3"
/db_xref="GeneID:854462"
CDS complement(852121..853077)
/gene="MPD1"
/locus_tag="YOR288C"
/EC_number="5.3.4.1"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0003756 protein
disulfide isomerase activity [PMID:16002399]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0015035
protein-disulfide reductase activity [PMID:16002399]"
/experiment="EXISTENCE:direct assay:GO:0019153
protein-disulfide reductase (glutathione) activity
[PMID:16002399]"
/experiment="EXISTENCE:genetic interaction:GO:0003756
protein disulfide isomerase activity [PMID:11157982]"
/experiment="EXISTENCE:genetic interaction:GO:0006457
protein folding [PMID:11157982]"
/experiment="EXISTENCE:genetic interaction:GO:0015035
protein-disulfide reductase activity [PMID:11157982]"
/experiment="EXISTENCE:mutant phenotype:GO:0003756 protein
disulfide isomerase activity [PMID:11157982]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:16002399]"
/note="Member of the protein disulfide isomerase (PDI)
family; interacts with and inhibits the chaperone activity
of Cne1p; MPD1 overexpression in a pdi1 null mutant
suppresses defects in Pdi1p functions such as
carboxypeptidase Y maturation"
/codon_start=1
/product="protein disulfide isomerase MPD1"
/protein_id="NP_014931.3"
/db_xref="GeneID:854462"
/db_xref="SGD:S000005814"
/translation="MLFLNIIKLLLGLFIMNEVKAQNFYDSDPHISELTPKSFDKAIH
NTNYTSLVEFYAPWCGHCKKLSSTFRKAAKRLDGVVQVAAVNCDLNKNKALCAKYDVN
GFPTLMVFRPPKIDLSKPIDNAKKSFSAHANEVYSGARTLAPIVDFSLSRIRSYVKKF
VRIDTLGSLLRKSPKLSVVLFSKQDKISPVYKSIALDWLGKFDFYSISNKKLKQLTDM
NPTYEKTPEIFKYLQKVIPEQRQSDKSKLVVFDADKDKFWEYEGNSINKNDISKFLRD
TFSITPNEGPFSRRSEYIAYLKTGKKPIKKNHSSSGNKHDEL"
gene <853357..>854112
/locus_tag="YOR289W"
/db_xref="GeneID:854463"
mRNA <853357..>854112
/locus_tag="YOR289W"
/product="uncharacterized protein"
/transcript_id="NM_001183708.1"
/db_xref="GeneID:854463"
CDS 853357..854112
/locus_tag="YOR289W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="hypothetical protein; transcription induced by the
unfolded protein response; green fluorescent protein
(GFP)-fusion protein localizes to both the cytoplasm and
the nucleus"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014932.1"
/db_xref="GeneID:854463"
/db_xref="SGD:S000005815"
/translation="MALRLFRKSSFFAKISMEGPKGSSPFAFYAFYQLYSHLNPGKSS
SLSLEDIRRRLYPDFKIDYNEKTSLFITWKKKSNKHHTIDTNEENYILRGCIGTFAKM
PIAHGIEKYSLIAALEDRRFSPIQKRELVDLKCSCNILGNFKTIFRGGGNPNGDIFDW
ELGKHGIELYFKHPKTGTTCSATFLPDVMPEQHWNKEDTFANLIEKAGYWGNISEVMD
NFETYFIEVIRYEGKKSSITYEEFNKQLKDIEA"
gene complement(854187..854259)
/locus_tag="YNCO0029C"
/db_xref="GeneID:854464"
tRNA complement(854187..854259)
/locus_tag="YNCO0029C"
/product="tRNA-Ala"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE
analysis; one of 5 nuclear tRNA genes containing the
tDNA-anticodon TGC (mature tRNA may be UGC or may contain
modified bases), decodes GCA and probably GCG codons into
alanine, one of 16 nuclear tRNAs for alanine"
/db_xref="GeneID:854464"
/db_xref="SGD:S000006525"
repeat_region 854275..854614
/note="Ty3 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007178"
gene complement(<855147..>860258)
/gene="SNF2"
/locus_tag="YOR290C"
/gene_synonym="GAM1; HAF1; SWI2; TYE3"
/db_xref="GeneID:854465"
mRNA complement(<855147..>860258)
/gene="SNF2"
/locus_tag="YOR290C"
/gene_synonym="GAM1; HAF1; SWI2; TYE3"
/product="SWI/SNF catalytic subunit SNF2"
/transcript_id="NM_001183709.3"
/db_xref="GeneID:854465"
CDS complement(855147..860258)
/gene="SNF2"
/locus_tag="YOR290C"
/gene_synonym="GAM1; HAF1; SWI2; TYE3"
/experiment="EXISTENCE:direct assay:GO:0000182 rDNA
binding [PMID:23437238]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:1901413|PMID:22842922|PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0006338 chromatin
remodeling [PMID:8016655]"
/experiment="EXISTENCE:direct assay:GO:0016514 SWI/SNF
complex
[PMID:18644858|PMID:8016655|PMID:8127913|PMID:8159677]"
/experiment="EXISTENCE:direct assay:GO:0031492 nucleosomal
DNA binding [PMID:18644858]"
/experiment="EXISTENCE:direct assay:GO:0140015 histone
H3K14ac reader activity [PMID:20126658]"
/experiment="EXISTENCE:direct assay:GO:0140658
ATP-dependent chromatin remodeler activity
[PMID:8127913|PMID:8458575]"
/experiment="EXISTENCE:genetic interaction:GO:0006338
chromatin remodeling [PMID:1459453]"
/experiment="EXISTENCE:genetic interaction:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:1901413]"
/experiment="EXISTENCE:mutant phenotype:GO:0006261
DNA-templated DNA replication [PMID:10198436]"
/experiment="EXISTENCE:mutant phenotype:GO:0006302
double-strand break repair [PMID:16024655]"
/experiment="EXISTENCE:mutant phenotype:GO:0006338
chromatin remodeling [PMID:8016655|PMID:1459453]"
/experiment="EXISTENCE:mutant phenotype:GO:0016514 SWI/SNF
complex [PMID:8159677]"
/experiment="EXISTENCE:mutant phenotype:GO:0031496
positive regulation of mating type switching
[PMID:6436497]"
/experiment="EXISTENCE:mutant phenotype:GO:0034198
cellular response to amino acid starvation
[PMID:10549298]"
/experiment="EXISTENCE:mutant phenotype:GO:0035973
aggrephagy [PMID:31846549]"
/experiment="EXISTENCE:mutant phenotype:GO:0042148 DNA
strand invasion [PMID:19766565]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:17681272|PMID:10549298|PMID:1339306|PMID:8016655|PMI
D:3542227]"
/experiment="EXISTENCE:mutant phenotype:GO:0140566 histone
reader activity [PMID:12419247]"
/experiment="EXISTENCE:mutant phenotype:GO:0140658
ATP-dependent chromatin remodeler activity
[PMID:8016655|PMID:8458575]"
/experiment="EXISTENCE:mutant phenotype:GO:1900189
positive regulation of cell adhesion involved in
single-species biofilm formation [PMID:18202364]"
/experiment="EXISTENCE:mutant phenotype:GO:2000219
positive regulation of invasive growth in response to
glucose limitation [PMID:18202364]"
/experiment="EXISTENCE:physical interaction:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:11865042]"
/note="Catalytic subunit of the SWI/SNF chromatin
remodeling complex; involved in transcriptional
regulation; contains DNA-stimulated ATPase activity;
functions interdependently in transcriptional activation
with Snf5p and Snf6p"
/codon_start=1
/product="SWI/SNF catalytic subunit SNF2"
/protein_id="NP_014933.3"
/db_xref="GeneID:854465"
/db_xref="SGD:S000005816"
/translation="MNIPQRQFSNEEVNRCYLRWQHLRNEHGMNAPSVPEFIYLTKVL
QFAAKQRQELQMQRQQQGISGSQQNIVPNSSDQAELPNNASSHISASASPHLAPNMQL
NGNETFSTSAHQSPIMQTQMPLNSNGGNNMLPQRQSSVGSLNATNFSPTPANNGENAA
EKPDNSNHNNLNLNNSELQPQNRSLQEHNIQDSNVMPGSQINSPMPQQAQMQQAQFQA
QQAQQAQQAQQAQQAQARLQQGRRLPMTMFTAEQSELLKAQITSLKCLVNRKPIPFEF
QAVIQKSINHPPDFKRMLLSLSEFARRRQPTDQNNQSNLNGGNNTQQPGTNSHYNNTN
TDNVSGLTRNAPLDSKDENFASVSPAGPSSVHNAKNGTLDKNSQTVSGTPITQTESKK
EENETISNVAKTAPNSNKTHTEQNNPPKPQKPVPLNVLQDQYKEGIKVVDIDDPDMMV
DSFTMPNISHSNIDYQTLLANSDHAKFTIEPGVLPVGIDTHTATDIYQTLIALNLDTT
VNDCLDKLLNDECTESTRENALYDYYALQLLPLQKAVRGHVLQFEWHQNSLLTNTHPN
FLSKIRNINVQDALLTNQLYKNHELLKLERKKTEAVARLKSMNKSAINQYNRRQDKKN
KRLKFGHRLIATHTNLERDEQKRAEKKAKERLQALKANDEEAYIKLLDQTKDTRITHL
LRQTNAFLDSLTRAVKDQQKYTKEMIDSHIKEASEEVDDLSMVPKMKDEEYDDDDDNS
NVDYYNVAHRIKEDIKKQPSILVGGTLKDYQIKGLQWMVSLFNNHLNGILADEMGLGK
TIQTISLLTYLYEMKNIRGPYLVIVPLSTLSNWSSEFAKWAPTLRTISFKGSPNERKA
KQAKIRAGEFDVVLTTFEYIIKERALLSKVKWVHMIIDEGHRMKNAQSKLSLTLNTHY
HADYRLILTGTPLQNNLPELWALLNFVLPKIFNSVKSFDEWFNTPFANTGGQDKIELS
EEETLLVIRRLHKVLRPFLLRRLKKDVEKELPDKVEKVVKCKMSALQQIMYQQMLKYR
RLFIGDQNNKKMVGLRGFNNQIMQLKKICNHPFVFEEVEDQINPTRETNDDIWRVAGK
FELLDRILPKLKATGHRVLIFFQMTQIMDIMEDFLRYINIKYLRLDGHTKSDERSELL
RLFNAPDSEYLCFILSTRAGGLGLNLQTADTVIIFDTDWNPHQDLQAQDRAHRIGQKN
EVRILRLITTNSVEEVILERAYKKLDIDGKVIQAGKFDNKSTSEEQEALLRSLLDAEE
ERRKKRESGVEEEEELKDSEINEILARNDEEMAVLTRMDEDRSKKEEELGVKSRLLEK
SELPDIYSRDIGAELKREESESAAVYNGRGARERKTATYNDNMSEEQWLRQFEVSDDE
KNDKQARKQRTKKEDKSEAIDGNGEIKGENIDADNDGPRINNISAEDRADTDLAMNDD
DFLSKKRKAGRPRGRPKKVKLEGSENSEPPALESSPVTGDNSPSEDFMDIPKPRTAGK
TSVKSARTSTRGRGRGRGRGRGRGRGRGRPPKARNGLDYVRTPAAATSPIDIREKVAK
QALDLYHFALNYENEAGRKLSDIFLSKPSKALYPDYYMIIKYPVAFDNINTHIETLAY
NSLKETLQDFHLIFSNARIYNTEGSVVYEDSLELEKVVTKKYCEIMGDNSQLDFTEFD
EQYGTRPLVLPPVVTSSVAESFTDEADSSMTEASV"
gene <861175..>865593
/gene="YPK9"
/locus_tag="YOR291W"
/db_xref="GeneID:854466"
mRNA <861175..>865593
/gene="YPK9"
/locus_tag="YOR291W"
/product="putative acid anhydride hydrolase"
/transcript_id="NM_001183710.1"
/db_xref="GeneID:854466"
CDS 861175..865593
/gene="YPK9"
/locus_tag="YOR291W"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:19001347|PMID:19182805]"
/experiment="EXISTENCE:direct assay:GO:1990816
vacuole-mitochondrion membrane contact site
[PMID:25026036]"
/note="Vacuolar protein; involved in oxidative stress
response and modulation of replicative lifespan; targeted
to vacuole via AP-3 pathway; undergoes
autophosphorylation, which is abolished by mutation of
Asp781 in catalytic motif (D781N); P5 ATPase subfamily
member with similarity to yeast Spf1p and homology to
human ATP13A2 (PARK9), mutations in which are associated
with Parkinson disease and Kufor-Rakeb syndrome"
/codon_start=1
/product="putative acid anhydride hydrolase"
/protein_id="NP_014934.1"
/db_xref="GeneID:854466"
/db_xref="SGD:S000005817"
/translation="MDIPSSNQIQHGQRSERNRRMPRASFSSTATTSTAATLTSAMVL
DQNNSEPYAGATFEAVPSSIVSFHHPHSFQSSNLPSPHSSGNLEQRGRRLTESEPLVL
SSAEQSRSSSRNPSHFRFFTQEQISNAEGASTLENTDYDMAWDATPAYEQDRIYGTGL
SSRRSSIRSFSRASSLSNAKSYGSFSKRGRSGSRAPQRLGENSDTGFVYHSATHSSSS
LSRYTTRERIPIELESQTDEILEDESSTHSLESSDSRRSASENNRGSFSGHDDVHNQH
SEYLKPDYHEKFYPQYAPNLHYQRFYIAEEDLVIGIAAYQTSKFWYIIYNLCCFLTFG
LVYLLTRWLPHLKVKLYGVKVPLAKAEWVVIENEFGEFVIQPIDRQWYNRPLSTVLPF
ENYPNPSYEPNDINLSHHHANEINPNVPILITFEYRYIKFIYSPLDDLFKTNNNWIDP
DWVDLSTVSNGLTKGVQEDRELAFGKNQINLRMKTTSEILFNEVLHPFYVFQVFSIIL
WGIDEYYYYAACIFLISVLSIFDSLNEQKKVSRNLAEMSHFHCDVRVLRDKFWTTISS
SELVPGDIYEVSDPNITILPCDSILLSSDCIVNESMLTGESVPVSKFPATEETMYQLC
DDFQSTQISSFVSKSFLYNGTNIIRARIAPGQTAALAMVVRTGFSTTKGSLVRSMVFP
KPTGFKFYRDSFKYIGFMSLIAIFGFCVSCVQFIKLGLDKKTMILRALDIITIVVPPA
LPATLTIGTNFALSRLKEKGIFCISPTRLNISGKIDVMCFDKTGTLTEDGLDVLGVQI
SEPNGVRGQKFGELLSDIRQVFPKFSLNDCSSPLDFKSRNFFMSLLTCHSLRSVDGNL
LGDPLDFKMFQFTGWSFEEDFQKRAFHSLYEGRHEDDVFPENSEIIPAVVHPDSNNRE
NTFTDNDPHNFLGVVRSFEFLSELRRMSVIVKTNNDDVYWSFTKGAPEVISEICNKST
LPADFEEVLRCYTHNGYRVIACAGKTLPKRTWLYSQKVSREEVESNLEFLGFIIFQNK
LKKETSETLKSLQDANIRTIMCTGDNILTAISVGREAGLIQCSRVYVPSINDTPLHGE
PVIVWRDVNEPDKILDTKTLKPVKLGNNSVESLRECNYTLAVSGDVFRLLFRDENEIP
EEYLNEILLNSSIYARMSPDEKHELMIQLQKLDYTVGFCGDGANDCGALKAADVGISL
SEAEASVAAPFTSKIFNISCVLDVIREGRAALVTSFACFQYMSLYSAIQFITITILYS
RGSNLGDFQFLYIDLLLIVPIAICMSWSKSYEKIDKKRPSANLVSPKILVPLLISVFL
VFLFQFIPWIIVQKMSWYIKPIVGGDDAVQSSDNTVLFFVSNFQYILTAIVLSVGPPY
REPMSKNFEFIVDITVSIGASLLLMTLDTESYLGKMLQLTPISNSFTMFIIVWVILNY
YAQLYIPPSIKGWLKKKKSSKKYKLLIQEEMKLKEV"
gene complement(<865653..>866582)
/locus_tag="YOR292C"
/db_xref="GeneID:854467"
mRNA complement(<865653..>866582)
/locus_tag="YOR292C"
/product="uncharacterized protein"
/transcript_id="NM_001183711.3"
/db_xref="GeneID:854467"
CDS complement(865653..866582)
/locus_tag="YOR292C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:14562095]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to the vacuole; YOR292C is
not an essential gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014935.3"
/db_xref="GeneID:854467"
/db_xref="SGD:S000005818"
/translation="MPLQLFGRDQIVVHYDNGNMSNDDQNHQSVLGSWTRRAAAALRT
LMNKRIQRITLTHWLLLVIWVTSLWKFTSHYRQLYANSAVFATLCTNILLFGISDILA
QSIACFYSYHVDPIPQILNDTFHHVQNNRDVENGGGYESDELSIFNDFTSEHSSYTDN
DDYPELDRPLATFKTDTFDFFRWGCFMFWGFFISFFQAPWYKFLNFFYTEDPTVVQVF
ERVLSDQLLYSPISLYCFFMFSNYVMEGGDKDTLGKKIQRLYISTLGCNYLVWPMVQF
INFLIMPRDFQAPFSSSVGVVWNCFLSMRNASK"
gene <867098..>867852
/gene="RPS10A"
/locus_tag="YOR293W"
/db_xref="GeneID:854468"
mRNA join(<867098..867149,867587..>867852)
/gene="RPS10A"
/locus_tag="YOR293W"
/product="40S ribosomal protein eS10 RPS10A"
/transcript_id="NM_001183712.1"
/db_xref="GeneID:854468"
CDS join(867098..867149,867587..867852)
/gene="RPS10A"
/locus_tag="YOR293W"
/experiment="EXISTENCE:genetic interaction:GO:0000054
ribosomal subunit export from nucleus [PMID:16246728]"
/note="Protein component of the small (40S) ribosomal
subunit; homologous to mammalian ribosomal protein S10, no
bacterial homolog; RPS10A has a paralog, RPS10B, that
arose from the whole genome duplication; mutations in the
human homolog associated with Diamond-Blackfan anemia"
/codon_start=1
/product="40S ribosomal protein eS10 RPS10A"
/protein_id="NP_014936.1"
/db_xref="GeneID:854468"
/db_xref="SGD:S000005819"
/translation="MLMPKEDRNKIHQYLFQEGVVVAKKDFNQAKHEEIDTKNLYVIK
ALQSLTSKGYVKTQFSWQYYYYTLTEEGVEYLREYLNLPEHIVPGTYIQERNPTQRPQ
RRY"
gene complement(<867998..>868147)
/locus_tag="YOR293C-A"
/db_xref="GeneID:1466486"
mRNA complement(<867998..>868147)
/locus_tag="YOR293C-A"
/product="uncharacterized protein"
/transcript_id="NM_001184669.3"
/db_xref="GeneID:1466486"
CDS complement(867998..868147)
/locus_tag="YOR293C-A"
/note="hypothetical protein; identified by expression
profiling and mass spectrometry"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878174.3"
/db_xref="GeneID:1466486"
/db_xref="SGD:S000028858"
/translation="MKLLFLNIIVVRRHLHCKSYRLSPWYIYIYGDYLLYTTEIPYKP
FTRQP"
gene <868340..>868951
/gene="RRS1"
/locus_tag="YOR294W"
/db_xref="GeneID:854469"
mRNA <868340..>868951
/gene="RRS1"
/locus_tag="YOR294W"
/product="ribosome biogenesis protein RRS1"
/transcript_id="NM_001183713.1"
/db_xref="GeneID:854469"
CDS 868340..868951
/gene="RRS1"
/locus_tag="YOR294W"
/experiment="EXISTENCE:direct assay:GO:0005654 nucleoplasm
[PMID:10688653]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:10688653]"
/experiment="EXISTENCE:direct assay:GO:0030687
preribosome, large subunit precursor
[PMID:17443350|PMID:17938242]"
/experiment="EXISTENCE:direct assay:GO:0034399 nuclear
periphery [PMID:21822217]"
/experiment="EXISTENCE:mutant phenotype:GO:0000055
ribosomal large subunit export from nucleus
[PMID:15135061]"
/experiment="EXISTENCE:mutant phenotype:GO:0000447
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from
5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10688653]"
/experiment="EXISTENCE:mutant phenotype:GO:0042273
ribosomal large subunit biogenesis [PMID:10688653]"
/note="Essential protein that binds ribosomal protein L11;
required for nuclear export of the 60S pre-ribosomal
subunit during ribosome biogenesis; localizes to the
nucleolus and in foci along nuclear periphery; cooperates
with Ebp2p and Mps3p to mediate telomere clustering by
binding Sir4p, but is not involved in telomere tethering;
mouse homolog shows altered expression in Huntington's
disease model mice"
/codon_start=1
/product="ribosome biogenesis protein RRS1"
/protein_id="NP_014937.1"
/db_xref="GeneID:854469"
/db_xref="SGD:S000005820"
/translation="MSAEDYKNLPVTVEKPIPVVYDLGNLAAFDSNVLDKNDLDSSNA
RREEKIKSLTRDNVQLLINQLLSLPMKTTTESVGGTGGQSSVMTLLQLPDPTTDLPRE
KPLPKAKAMTKWEKFAAKKGIKPKERAGKMIYDEASGEWVPKWGYKGANKKLDDQWLV
EVDDKVKGTDNELIDPRTLNRAERKRLVKKNEKQQRRNMKNAL"
gene <869208..>869894
/gene="UAF30"
/locus_tag="YOR295W"
/db_xref="GeneID:854470"
mRNA <869208..>869894
/gene="UAF30"
/locus_tag="YOR295W"
/product="Uaf30p"
/transcript_id="NM_001183714.3"
/db_xref="GeneID:854470"
CDS 869208..869894
/gene="UAF30"
/locus_tag="YOR295W"
/experiment="EXISTENCE:direct assay:GO:0000500 RNA
polymerase I upstream activating factor complex
[PMID:11500378]"
/experiment="EXISTENCE:direct assay:GO:0001165 RNA
polymerase I cis-regulatory region sequence-specific DNA
binding [PMID:18451108]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:22362748]"
/experiment="EXISTENCE:mutant phenotype:GO:0001181 RNA
polymerase I general transcription initiation factor
activity [PMID:18765638]"
/experiment="EXISTENCE:mutant phenotype:GO:0006325
chromatin organization [PMID:20154141]"
/experiment="EXISTENCE:mutant phenotype:GO:0042790
nucleolar large rRNA transcription by RNA polymerase I
[PMID:11500378|PMID:18765638]"
/note="Subunit of UAF (upstream activation factor)
complex; UAF is an RNA polymerase I specific transcription
stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p,
Rrn10p, histones H3 and H4; targeting factor for the UAF
that facilitates activation of many rDNA genes; deletion
decreases cellular growth rate; UAF30 has a paralog, TRI1,
that arose from the whole genome duplication"
/codon_start=1
/product="Uaf30p"
/protein_id="NP_014938.3"
/db_xref="GeneID:854470"
/db_xref="SGD:S000005821"
/translation="MAELNDYSTMIDILLSDMDLETVTTKKVRMALKEVYAIDVESQG
KAINKLIRKHLDLVKERPRFERSLEDLLKENATLAIELTKEITVSKRSSGEEKNDSET
KGTHVEKKKGTVSKSPISTRKVTLSKSLASLLGEHELTRTEVVRRLWAYIKAHNLQNP
NNKKEILCDEKLELILGKSTNMFEMHKILASHMTEPKKISDCPPLIQEVRRKEKPIVS
DSEQSDTKGI"
gene <870202..>874071
/locus_tag="YOR296W"
/db_xref="GeneID:854471"
mRNA <870202..>874071
/locus_tag="YOR296W"
/product="uncharacterized protein"
/transcript_id="NM_001183715.1"
/db_xref="GeneID:854471"
CDS 870202..874071
/locus_tag="YOR296W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to the cytoplasm; expressed
during copper starvation; YOR296W is not an essential
gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014939.1"
/db_xref="GeneID:854471"
/db_xref="SGD:S000005822"
/translation="MNRTVSTLSSTVSDVSVEIPSICNVINTELPTSDVYLYTLKLIL
LDYINEPRFKEAALLSNRTGTSRVLSDKTNHQQTQHGKKLVVDKQDDMSERDIVQATL
RILKGKLAQISGNKNLAPNEMHWKSIVKMYYSMLDSSSADTFSKMGQMEEVVGYFTNI
ASNELKKMTIKNSRDELFSEVAYFIDLVIDVLPDSCANIIKRLLDYKINLKKGETTVK
KKRAASPATVPQYRSISGSTISNKQPSFKVQDISHMKYFMQLFETDETKLHQDVMAVK
DDCTNPIFCGELRYLRKKIKKDNGTLTASDFSSDREYNLWKNYELLEIANLMDRFEIG
EKVTSHGNRLIPKDAKSVFVRLIGLVLKKECSNAVNAINLSQEALFFFHKSARYWRIE
YPSTISSLVYSAANLSVLGDEELNIPITENLFSVIRNKYLCSEDNLDPSAWNAQDRYL
WAANLFHTTDQSMRTINNLLTAIFSGTKPKFSPVLSFYYSNIVGDPVMEFYETQSVAV
KKYWIKLFKKTLFKASEDYFVSLLQDMLKANAIEIQNVQNLVETIIEAIKAIQKRYNK
PLLDEISLPRQCAVFLCEVYGSDSLNLIKTAEKSTMKMTGQKLGPIDALDMYDVLKEL
RQIYLQVKPKGKFFFNLENYFIKYLTRLCDDVSRNVQKVIKSSLESENWQPVNDQDHF
SRSVLDIFKMINESTSMLEKFGWQNEFQLAQMITVILKAFSDGMLSYSAQLMELIQRD
LQEGDEPSYSLESSDTRSSLSLNNANVNHEKSRSSRLFEDLKNVVKSTPKMVAPAPYQ
FKKRTCVLLNDLDKTLFLLESFEEKADPSKISSVIAQYHSSHNLEDNGKSFDDQNMKQ
VYTLRIIGAENIKGFSKTGLSNTYVSMRNITLQREIGTTKIVARSITPKWDEEFVFES
PFGKSNDIMFTIWHHPHSRLKNLAEDDLCGKANMKFTPRKLKDDGFPIDFSLTLNPQG
TLYCQISLESEKIDAVSSMGRIYRSFSRSRDRAINLIVNKFSDFIAFAFSRTTLKTVC
GHHGSALASDEAVYDAILPLFDYLNANLNILASELSQRLLFMVMLRAWNLVLENADLL
LLPALNSAKVNILRSAKKSLWENTLSTTKTVSGYGRPLTQAEIEAVFKWLDALCVDFF
HNKGEGPPLAELKNEYYQNILLIPAFYDKSVSELKDEVQRLIPLYEEYLRWFYLKKTP
ITFTNKSAGTISRKKSLVANIVKEPKEQLERDAEVMNIILRILIAKGQHDYVHRILHD
RKELVNTMKNRRAVSRAVTPTGKKGRN"
rep_origin 874251..874412
/note="ARS1526; Autonomously Replicating Sequence"
/db_xref="SGD:S000118494"
gene complement(<874743..>875321)
/gene="TIM18"
/locus_tag="YOR297C"
/db_xref="GeneID:854472"
mRNA complement(<874743..>875321)
/gene="TIM18"
/locus_tag="YOR297C"
/product="Tim18p"
/transcript_id="NM_001183716.4"
/db_xref="GeneID:854472"
CDS complement(874743..875321)
/gene="TIM18"
/locus_tag="YOR297C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0008320 protein
transmembrane transporter activity [PMID:12637749]"
/experiment="EXISTENCE:direct assay:GO:0042721 TIM22
mitochondrial import inner membrane insertion complex
[PMID:10637294|PMID:10648604]"
/experiment="EXISTENCE:genetic interaction:GO:0045039
protein insertion into mitochondrial inner membrane
[PMID:10637294]"
/experiment="EXISTENCE:mutant phenotype:GO:0006915
apoptotic process [PMID:17922641]"
/experiment="EXISTENCE:mutant phenotype:GO:0006970
response to osmotic stress [PMID:17922641]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress [PMID:17922641]"
/experiment="EXISTENCE:mutant phenotype:GO:0046685
response to arsenic-containing substance [PMID:17922641]"
/note="Component of the mitochondrial TIM22 complex;
involved in insertion of polytopic proteins into the inner
membrane; may mediate assembly or stability of the
complex"
/codon_start=1
/product="Tim18p"
/protein_id="NP_014940.4"
/db_xref="GeneID:854472"
/db_xref="SGD:S000005823"
/translation="MLLFPGLKPVLNASTVIVNPVRAVFPGLVLSTKRSFYSINRLNA
ENKINDIANTSKEASSSVQMFKPPEFSQFKDSYQKDYERIAKYTLIPLTMVPFYASFT
GGVINPLLDASLSSIFLIYLQYGFTSCIIDYIPKEKYPRWHKLALYCLYGGSMLSLYG
IYELETKNNGFVDLVKKLWNENDDHLYIFGRN"
gene <875599..>877038
/gene="MUM3"
/locus_tag="YOR298W"
/db_xref="GeneID:854473"
mRNA <875599..>877038
/gene="MUM3"
/locus_tag="YOR298W"
/product="Mum3p"
/transcript_id="NM_001183717.1"
/db_xref="GeneID:854473"
CDS 875599..877038
/gene="MUM3"
/locus_tag="YOR298W"
/experiment="EXISTENCE:mutant phenotype:GO:0030476
ascospore wall assembly [PMID:15590821]"
/note="hypothetical protein involved in outer spore wall
organization; has similarity to the tafazzins superfamily
of acyltransferases"
/codon_start=1
/product="Mum3p"
/protein_id="NP_014941.1"
/db_xref="GeneID:854473"
/db_xref="SGD:S000005824"
/translation="MGFVDFFETYMVGSRVQFKQLDISDWLSLTPRLLILFGYFYLHS
FFTAINQFLQFINTNSFCLRLHLLYDRFWSHVPIIGEYKIRLLSRALTYSKLKIIPTL
DKVLEAIEIWFQLHLVEMTFEKKKNVQIFITEGSDDLNFFKDSKFQTTLMICNHRSVN
DYTLINYLFLKSCPTKFYTKWEFLQKLRKGEDLAEWPQLKFLGWGKMFNFPRLDLLKN
IFFKDETLALSSNELRDILERQNNQAITIFPEVNIMSLELSIIQRKLHQDFPFVINFY
NLLYPRFKNFTTLMAAFSSIKNIKRKKNRNNIIKEARYLFHRELDKLVHKSMKMESSK
VSDKTTPPMIVDNSYLLTKKEEISSGKPKVVRINPYIYDVTIIYYRVKYTDSGHDHTN
GDLRLHKGYQLEQISPTIFEMIQPEMESENNIKDKDPIVVMVNVKKHQIQPLLAYNDE
SLEKWLENRWIEKDRLIESLQKNIKIETK"
gene complement(<877230..>877685)
/gene="MBF1"
/locus_tag="YOR298C-A"
/gene_synonym="SUF13"
/db_xref="GeneID:854474"
mRNA complement(<877230..>877685)
/gene="MBF1"
/locus_tag="YOR298C-A"
/gene_synonym="SUF13"
/product="multiprotein-bridging factor 1"
/transcript_id="NM_001184346.3"
/db_xref="GeneID:854474"
CDS complement(877230..877685)
/gene="MBF1"
/locus_tag="YOR298C-A"
/gene_synonym="SUF13"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:39566505]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:39566505]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:39566505]"
/experiment="EXISTENCE:genetic interaction:GO:0140469
GCN2-mediated signaling [PMID:39566505]"
/experiment="EXISTENCE:mutant phenotype:GO:0140469
GCN2-mediated signaling [PMID:39566505]"
/note="Core integrated stress response (ISR) factor;
optimizes ribosome collision-mediated Gcn2 activation and
the downstream Gcn4-mediated transcriptional response;
required for optimal stress-induced eIF2alpha (Sui2p)
phosphorylation and downstream de-repression of GCN4
translation; Mbf1p is recruited to collided disomes and
trisomes during the ISR; suppressor of frameshift
mutations; protein abundance increases in response to DNA
replication stress; homologous to human EDF1"
/codon_start=1
/product="multiprotein-bridging factor 1"
/protein_id="NP_014942.4"
/db_xref="GeneID:854474"
/db_xref="SGD:S000007253"
/translation="MSDWDTNTIIGSRARAGGSGPRANVARSQGQINAARRQGLVVSV
DKKYGSTNTRGDNEGQRLTKVDRETDIVKPKKLDPNVGRAISRARTDKKMSQKDLATK
INEKPTVVNDYEAARAIPNQQVLSKLERALGVKLRGNNIGSPLGAPKKK"
gene <878435..>880675
/gene="BUD7"
/locus_tag="YOR299W"
/db_xref="GeneID:854475"
mRNA <878435..>880675
/gene="BUD7"
/locus_tag="YOR299W"
/product="exomer complex subunit"
/transcript_id="NM_001183718.1"
/db_xref="GeneID:854475"
CDS 878435..880675
/gene="BUD7"
/locus_tag="YOR299W"
/experiment="EXISTENCE:direct assay:GO:0030140 trans-Golgi
network transport vesicle [PMID:16498409]"
/experiment="EXISTENCE:direct assay:GO:0034044 exomer
complex [PMID:17000877]"
/experiment="EXISTENCE:genetic interaction:GO:0006893
Golgi to plasma membrane transport [PMID:16498409]"
/experiment="EXISTENCE:mutant phenotype:GO:0000282
cellular bud site selection [PMID:8657162]"
/experiment="EXISTENCE:physical interaction:GO:0006031
chitin biosynthetic process [PMID:16498409]"
/note="Member of the ChAPs family (Chs5p-Arf1p-binding
proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p;
ChAPs family proteins form the exomer complex with Chs5p
to mediate export of specific cargo proteins, including
Chs3p, from the Golgi to the plasma membrane; BUD7 has a
paralog, BCH1, that arose from the whole genome
duplication"
/codon_start=1
/product="exomer complex subunit"
/protein_id="NP_014943.1"
/db_xref="GeneID:854475"
/db_xref="SGD:S000005825"
/translation="MITQNSIPEVKEDFIGYALHERRIRLPQFQDLGPADLVTLTKYL
PTSSNTNAINSTSRNGAAIIQSPAAVVADDSAASMATNGDASDTAVTTNYTNASIYSS
SRNANDGAPMVAELHPLDKLKDEVGTFFYSMGVDTSGPTSIAIFLKEISEVISEKPQV
WFGRKKTFNVARISFSTWNAFRRCDINVVVHIPGSIQNFIVDCNGESQNIEMCADYDL
IWAETFVSGVVRSIMLMKENAEEGELQNLVETLILNPFTAGQIDDVPEMFIDLFPIVY
HKGPLLGAPYYITNVTNTNNYLVETLVEIVKLTRNVSRAEIMLKNLATDNPEAIIILI
KIFLVCDQELDAIKLTYDMLSQDKIINNTNNRMDYKSELLCLQAQFLIDKRQDYSLAQ
NIAQEAVNCSPSEFRPWYLLSKVYVKLNDIENALLILNSCPMSPLKEKYVLKRVAPLP
SNNSLHLPLPIDVVLDEVTSLNPQDVQNEHRSADPMLVNLAASNLKSTFQLAYRLLTE
IVQITGWENLLKYRSNIFVMEEEYQKSSSSLPKDVNKQEEQPLRAKRLCERWLDNLFM
LLYEDLKMYTLWQTEQLYMDAQNNNHNKLTFEWELFGLCARRLGHFPEAAKAFQNGLS
QRFSSRCARKLLEYCINERQRVKNFINSPNSHDMVPEIVSSRIRELDNSIIDLCVKIC
CWNHRWYTEFSISLLDCLSVVIQDMSLTKVSNEISSRYPETVLNLVQENLLNFFTTCT
IGCYDA"
gene <880965..>882272
/gene="RAX1"
/locus_tag="YOR301W"
/db_xref="GeneID:854477"
mRNA <880965..>882272
/gene="RAX1"
/locus_tag="YOR301W"
/product="Rax1p"
/transcript_id="NM_001183720.3"
/db_xref="GeneID:854477"
CDS 880965..882272
/gene="RAX1"
/locus_tag="YOR301W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005621 cellular
bud scar [PMID:25416945|PMID:15356260]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:15356260|PMID:14980713]"
/experiment="EXISTENCE:genetic interaction:GO:0007121
bipolar cellular bud site selection [PMID:14980713]"
/experiment="EXISTENCE:mutant phenotype:GO:0007120 axial
cellular bud site selection [PMID:29074565]"
/experiment="EXISTENCE:mutant phenotype:GO:0007121 bipolar
cellular bud site selection [PMID:14980713]"
/experiment="EXISTENCE:mutant phenotype:GO:0008104
intracellular protein localization [PMID:25416945]"
/note="Protein involved in establishing bud site
selection; localizes to the bud neck and previous sites of
cell division (bud scars or cytokinesis remnants (CRMs);
localization to both sites is interdependent with Rax2p;
required with Rax2p to anchor Nba1p and Nis1p to CRMs
where a Cdc42p inhibitory zone is established, preventing
repolarization of cells at previous division sites;
predicted type IIIa transmembrane protein with similarity
to members of the insulin-related peptide superfamily"
/codon_start=1
/product="Rax1p"
/protein_id="NP_014945.3"
/db_xref="GeneID:854477"
/db_xref="SGD:S000005827"
/translation="MKEELSKVSSMQNFEMIQRERLPTLYEVLIQRTSQPVDLWTFYT
FLSQFPYAINYLDFWVDLMTHTRLCKNYIELVRKSLINFPQEQQQNGSTSTATFDLLN
ALIEEGHLDPEAPDKLLENSGPDVPFSPKLNELLGDWKHQSGIGQEALRNEDVALIVD
EIMKRRSQQDGKPQITTKQLLHSAVGLCNTYLVSPEQSERYLSNIPMETRNRIIESVQ
IERKYDIEIFDDLKNLTYQFLEMDCFPKFLSRVALHNIHDEISDWRFHSVGVTNEKSN
RSRGQTHISRSPFSNHTSISRIGFGLLWLGIGFWIGYVLIFLAYSRAIRVVTVVPFTL
GCYCIVCGMYQVDIVYSWFGVTQRLLHRHKNAGNDEGDASSDTDHVPMILAVFGGRRR
LTRIEHPFTRQLLRKRGLWCLLLVVGATAAFTVIFSCVPGRRV"
gene <882765..>882842
/locus_tag="YOR302W"
/db_xref="GeneID:854478"
mRNA <882765..>882842
/locus_tag="YOR302W"
/product="uncharacterized protein"
/transcript_id="NM_001183721.1"
/db_xref="GeneID:854478"
CDS 882765..882842
/locus_tag="YOR302W"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:10608810]"
/experiment="EXISTENCE:direct assay:GO:0006417 regulation
of translation [PMID:10608810]"
/note="CPA1 uORF; Arginine attenuator peptide, regulates
translation of the CPA1 mRNA"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014946.1"
/db_xref="GeneID:854478"
/db_xref="SGD:S000005828"
/translation="MFSLSNSQYTCQDYISDHIWKTSSH"
gene <882899..>884134
/gene="CPA1"
/locus_tag="YOR303W"
/db_xref="GeneID:854479"
mRNA <882899..>884134
/gene="CPA1"
/locus_tag="YOR303W"
/product="carbamoyl-phosphate synthase
(glutamine-hydrolyzing) CPA1"
/transcript_id="NM_001183722.3"
/db_xref="GeneID:854479"
CDS 882899..884134
/gene="CPA1"
/locus_tag="YOR303W"
/EC_number="6.3.5.5"
/experiment="EXISTENCE:direct assay:GO:0004088
carbamoyl-phosphate synthase (glutamine-hydrolyzing)
activity [PMID:8626695]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005951
carbamoyl-phosphate synthase complex [PMID:206535]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405]"
/experiment="EXISTENCE:mutant phenotype:GO:0004088
carbamoyl-phosphate synthase (glutamine-hydrolyzing)
activity [PMID:6086650]"
/experiment="EXISTENCE:mutant phenotype:GO:0006526
L-arginine biosynthetic process [PMID:9290206]"
/note="Small subunit of carbamoyl phosphate synthetase;
carbamoyl phosphate synthetase catalyzes a step in the
synthesis of citrulline, an arginine precursor;
translationally regulated by an attenuator peptide encoded
by YOR302W within the CPA1 mRNA 5'-leader"
/codon_start=1
/product="carbamoyl-phosphate synthase
(glutamine-hydrolyzing) CPA1"
/protein_id="NP_014947.3"
/db_xref="GeneID:854479"
/db_xref="SGD:S000005829"
/translation="MSSAATKATFCIQNGPSFEGISFGANKSVAGETVFTTSLVGYPE
SMTDPSYRGQILVFTQPLIGNYGVPSGEARDEYNLLKYFESPHIHVVGIVVAEYAYQY
SHWTAVESLAQWCQREGVAAITGVDTRELVQYLREQGSSLGRITLADHDPVPYVNPMK
TNLVAQVTTKKPFHVSALPGKAKANVALIDCGVKENIIRCLVKRGANVTVFPYDYRIQ
DVASEFDGIFLSNGPGNPELCQATISNVRELLNNPVYDCIPIFGICLGHQLLALASGA
STHKLKYGNRAHNIPAMDLTTGQCHITSQNHGYAVDPETLPKDQWKPYFVNLNDKSNE
GMIHLQRPIFSTQFHPEAKGGPLDTAILFDKFFDNIEKYQLQSQAKSSISLKVTYSTD
KSRLQSINVTKLAKERVLF"
gene <884514..>887876
/gene="ISW2"
/locus_tag="YOR304W"
/db_xref="GeneID:854480"
mRNA <884514..>887876
/gene="ISW2"
/locus_tag="YOR304W"
/product="DNA translocase"
/transcript_id="NM_001183724.1"
/db_xref="GeneID:854480"
CDS 884514..887876
/gene="ISW2"
/locus_tag="YOR304W"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:16227570]"
/experiment="EXISTENCE:direct assay:GO:0003697
single-stranded DNA binding [PMID:19203228]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005880 nuclear
microtubule [PMID:10620773]"
/experiment="EXISTENCE:direct assay:GO:0006338 chromatin
remodeling [PMID:16227570]"
/experiment="EXISTENCE:direct assay:GO:0008623 CHRAC
[PMID:14673157]"
/experiment="EXISTENCE:direct assay:GO:0015616 DNA
translocase activity [PMID:19203228]"
/experiment="EXISTENCE:direct assay:GO:0019237 centromeric
DNA binding [PMID:23468649]"
/experiment="EXISTENCE:genetic interaction:GO:0006369
termination of RNA polymerase II transcription
[PMID:12504018]"
/experiment="EXISTENCE:genetic interaction:GO:0060195
negative regulation of antisense RNA transcription
[PMID:18075583]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:12624196]"
/experiment="EXISTENCE:mutant phenotype:GO:0000183 rDNA
heterochromatin formation [PMID:17689493]"
/experiment="EXISTENCE:mutant phenotype:GO:0006338
chromatin remodeling [PMID:11238381|PMID:16227570]"
/experiment="EXISTENCE:mutant phenotype:GO:0031509
subtelomeric heterochromatin formation [PMID:14673157]"
/experiment="EXISTENCE:mutant phenotype:GO:0071444
cellular response to pheromone [PMID:12624196]"
/note="ATP-dependent DNA translocase involved in chromatin
remodeling; ATPase component that, with Itc1p, forms a
complex required for repression of a-specific genes, INO1,
and early meiotic genes during mitotic growth; exhibits
basal levels of chromatin binding throughout the genome as
well as interacts with sequence-specific factors to
accurately position nucleosomes in chromatin; targeted by
Ume6p- and Sua7p-dependent DNA looping to many loci
genome-wide"
/codon_start=1
/product="DNA translocase"
/protein_id="NP_014948.1"
/db_xref="GeneID:854480"
/db_xref="SGD:S000005831"
/translation="MTTQQEEQRSDTKNSKSESPSEVLVDTLDSKSNGSSDDDNIGQS
EELSDKEIYTVEDRPPEYWAQRKKKFVLDVDPKYAKQKDKSDTYKRFKYLLGVTDLFR
HFIGIKAKHDKNIQKLLKQLDSDANKLSKSHSTVSSSSRHHRKTEKEEDAELMADEEE
EIVDTYQEDIFVSESPSFVKSGKLRDYQVQGLNWLISLHENKLSGILADEMGLGKTLQ
TISFLGYLRYVKQIEGPFLIIVPKSTLDNWRREFLKWTPNVNVLVLHGDKDTRADIVR
NIILEARFDVLITSYEMVIREKNALKRLAWQYIVIDEAHRIKNEQSALSQIIRLFYSK
NRLLITGTPLQNNLHELWALLNFLLPDIFGDSELFDEWFEQNNSEQDQEIVIQQLHSV
LNPFLLRRVKADVEKSLLPKIETNVYVGMTDMQIQWYKSLLEKDIDAVNGAVGKREGK
TRLLNIVMQLRKCCNHPYLFEGAEPGPPYTTDEHLIFNSGKMIILDKLLKRLKEKGSR
VLIFSQMSRLLDILEDYCYFRDFEYCRIDGSTSHEERIEAIDEYNKPNSEKFVFLLTT
RAGGLGINLVTADTVILFDSDWNPQADLQAMDRAHRIGQKKQVHVYRFVTENAIEEKV
IERAAQKLRLDQLVIQQGTGKKTASLGNSKDDLLDMIQFGAKNMFEKKASKVTVDADI
DDILKKGEQKTQELNAKYQSLGLDDLQKFNGIENQSAYEWNGKSFQKKSNDKVVEWIN
PSRRERRREQTTYSVDDYYKEIIGGGSKSASKQTPQPKAPRAPKVIHGQDFQFFPKEL
DALQEKEQLYFKKKVNYKVTSYDITGDIRNEGSDAEEEEGEYKNAANTEGHKGHEELK
RRIEEEQEKINSAPDFTQEDELRKQELISKAFTNWNKRDFMAFINACAKYGRDDMENI
KKSIDSKTPEEVEVYAKIFWERLKEINGWEKYLHNVELGEKKNEKLKFQETLLRQKIE
QCKHPLHELIIQYPPNNARRTYNTLEDKFLLLAVNKYGLRADKLYEKLKQEIMMSDLF
TFDWFIKTRTVHELSKRVHTLLTLIVREYEQPDANKKKRSRTSATREDTPLSQNESTR
ASTVPNLPTTMVTNQKDTNDHVDKRTKIDQEA"
gene complement(<888520..>888750)
/gene="BIL1"
/locus_tag="YOR304C-A"
/db_xref="GeneID:854481"
mRNA complement(<888520..>888750)
/gene="BIL1"
/locus_tag="YOR304C-A"
/product="Bil1p"
/transcript_id="NM_001183723.3"
/db_xref="GeneID:854481"
CDS complement(888520..888750)
/gene="BIL1"
/locus_tag="YOR304C-A"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005933 cellular
bud [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:23671312]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:14562095|PMID:23671312]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807]"
/experiment="EXISTENCE:direct assay:GO:0051017 actin
filament bundle assembly [PMID:23671312]"
/note="Protein that binds Bud6p and has a role in actin
cable assembly; involved in the Bnr1p-dependent pathway of
cable assembly; localizes to bud tip and bud neck"
/codon_start=1
/product="Bil1p"
/protein_id="NP_014949.3"
/db_xref="GeneID:854481"
/db_xref="SGD:S000005830"
/translation="MSTEKLEASEEPQAPLANTSETNSIKGDTENIVTVFDLANEIEK
SLKDVQRQMKENDDEFSRSIQAIEDKLNKMSR"
gene <889022..>889750
/gene="RRG7"
/locus_tag="YOR305W"
/db_xref="GeneID:854482"
mRNA <889022..>889750
/gene="RRG7"
/locus_tag="YOR305W"
/product="Rrg7p"
/transcript_id="NM_001183725.1"
/db_xref="GeneID:854482"
CDS 889022..889750
/gene="RRG7"
/locus_tag="YOR305W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to the mitochondrion;
deletion confers sensitivity to
4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO);
YOR305W is not an essential gene"
/codon_start=1
/product="Rrg7p"
/protein_id="NP_014950.1"
/db_xref="GeneID:854482"
/db_xref="SGD:S000005832"
/translation="MIKNYLGRRWLNNPAIQAYVKQNAAVAHSTVFQGNLYEYTVMRE
LSEKLRMTKLRKTGGAHDGGVDIKGSWPVDDIYWKISSLMPNLEMASNIKRTNSQNGF
VLKPLKYRIIDHTFEPLKVLVQCKAFTKSKLSPREFRELVGTFTSLVSHSQRNKTVCI
MCSPHMLTKDTLNLINNITLPLIYLRVEMLKEKTDGHFDLINSGKLINYYENSYASTL
MQDCKISEWLKLKLYKNSDFNSEK"
gene complement(<889867..>891432)
/gene="MCH5"
/locus_tag="YOR306C"
/db_xref="GeneID:854483"
mRNA complement(<889867..>891432)
/gene="MCH5"
/locus_tag="YOR306C"
/product="riboflavin transporter"
/transcript_id="NM_001183726.4"
/db_xref="GeneID:854483"
CDS complement(889867..891432)
/gene="MCH5"
/locus_tag="YOR306C"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16204239]"
/experiment="EXISTENCE:genetic interaction:GO:0032217
riboflavin transmembrane transporter activity
[PMID:16204239]"
/experiment="EXISTENCE:genetic interaction:GO:0032218
riboflavin transport [PMID:16204239]"
/note="Plasma membrane riboflavin transporter; facilitates
the uptake of vitamin B2; required for FAD-dependent
processes; sequence similarity to mammalian
monocarboxylate permeases, however mutants are not
deficient in monocarboxylate transport"
/codon_start=1
/product="riboflavin transporter"
/protein_id="NP_014951.4"
/db_xref="GeneID:854483"
/db_xref="SGD:S000005833"
/translation="MSSDSLTPKDTIVPEEQTNQLRQPDLDEDSIHYDPEADDLESLE
TTASYASTSVSAKVYTKKEVNKGTDIESQPHWGENTSSTHDSDKEEDSNEEIESFPEG
GFKAWVVTFGCFLGLIACFGLLNSTGVIESHLQDNQLSSESVSTIGWLFSLFLFVCSA
SCIISGTYFDRNGFRTIMIVGTVFHVAGLFATANSTKYWHFILSFAIVCGFGNGIVLS
PLVSVPAHYFFKRRGTALAMATIGGSVGGVVFPIMLRSFFSMKSDTDPTYGFVWGIRT
LGFLDLALLTLSIILVKERLPHVIENSKDGESRWRYILRVYILQCFDAKAFLDMKYLF
CVLGTVFSELSINSALTYYGSYATSHGISANDAYTLIMIINVCGIPGRWVPGYLSDKF
GRFNVAIATLLTLFIVMFVGWLPFGTNLTNMYVISALYGFCSGSVFSLLPVCCGQISK
TEEFGKRYSTMYFVVGFGTLVGIPITGAIISIKTTADYQHYIIFCGLATFVSAVCYII
SRAYCVGFKWVRF"
gene complement(<892731..>894092)
/gene="SLY41"
/locus_tag="YOR307C"
/db_xref="GeneID:854484"
mRNA complement(<892731..>894092)
/gene="SLY41"
/locus_tag="YOR307C"
/product="Sly41p"
/transcript_id="NM_001183727.1"
/db_xref="GeneID:854484"
CDS complement(892731..894092)
/gene="SLY41"
/locus_tag="YOR307C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095|PMID:23613772]"
/experiment="EXISTENCE:genetic interaction:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:1903839]"
/note="Protein involved in ER-to-Golgi transport; packaged
into COPII vesicles for trafficking between ER and Golgi"
/codon_start=1
/product="Sly41p"
/protein_id="NP_014952.1"
/db_xref="GeneID:854484"
/db_xref="SGD:S000005834"
/translation="MIQTQSTAIKRRNSVHKNLFDPSLYQIPEPPRGGFQHQKKEYSK
ETFSNQVFGYDITSLKKRFTQLFPSNIQGYLPEVDLRITIICSIWYVTSSISSNLSKA
ILRTFNHPIALTELQFLVSAVLCVGFASIVNLFRLPRLKHTKFSKALNSFPDGILPEY
LDGNFRSSILHKFLVPSKLVLMTTFPMGIFQFIGHITSHKAVSMIPVSLVHSVKALSP
IITVGYYKFFEHRYYNSMTYYTLLLLIFGVMTTCWSTHGSKRASDNKSGSSLIGLLFA
FISMIIFVAQNIFAKNILTIRRKVGILPSSSTDDVTSKEGQPSLDKTRFSPLQVDKIT
ILFYCSCIGFSLTLLPFLTGELMHGGSVINDLTLETVALVAIHGIAHFFQAMLAFQLI
GLLSSINYSVANIMKRIVVISVALFWETKLNFFQVFGVILTIAGLYGYDKWGLSKKDG
RQA"
gene complement(<894624..>896387)
/gene="SNU66"
/locus_tag="YOR308C"
/db_xref="GeneID:854485"
mRNA complement(<894624..>896387)
/gene="SNU66"
/locus_tag="YOR308C"
/product="U4/U6-U5 snRNP complex subunit SNU66"
/transcript_id="NM_001183728.3"
/db_xref="GeneID:854485"
CDS complement(894624..896387)
/gene="SNU66"
/locus_tag="YOR308C"
/experiment="EXISTENCE:direct assay:GO:0046540 U4/U6 x U5
tri-snRNP complex [PMID:10377396|PMID:10449419]"
/experiment="EXISTENCE:genetic interaction:GO:0000481
maturation of 5S rRNA [PMID:19806183]"
/experiment="EXISTENCE:mutant phenotype:GO:0000398 mRNA
splicing, via spliceosome [PMID:10449419|PMID:11720284]"
/experiment="EXISTENCE:mutant phenotype:GO:0000481
maturation of 5S rRNA [PMID:19806183]"
/note="Component of the U4/U6.U5 snRNP complex; involved
in pre-mRNA splicing via spliceosome; also required for
pre-5S rRNA processing and may act in concert with Rnh70p;
has homology to human SART-1"
/codon_start=1
/product="U4/U6-U5 snRNP complex subunit SNU66"
/protein_id="NP_014953.3"
/db_xref="GeneID:854485"
/db_xref="SGD:S000005835"
/translation="MNKTENLSIEETNEIREKLGMKPIPVFQEKNTDHKESLSIEETN
ELRASLGLKLIPPQQNFNSSPPNVHNTSKIDELREKITKFQKKANAPLRMAHLLEETN
VNDDSSWLENMDAIPSSHESKRSSTLPRKGATKEDENIDLHNVQVSYNIEALSPKKDT
ILTLKESSIFDDTDSTEVLENVKAAEENADREKLRLRQMNKDRRQKKKILNVSSLDIE
EEEEGEKHSITTTHLIIGAEQGVMKAPNTISAKPPTGKVKVNFDSANNMSDEDGGDFK
PLKIKKRKIKDPRSTKARKSKITDKMEIVKLVDEDESLSWMEEEQPVTIINPRTSSNN
ELKGPEDLAREIEKARDEEKRRTESILKMREISNSIVVDEKVTFLNTLDTSLSERSAT
ENKVKVHGEGEKNIGDVTNGHTKEGSGNNTLTEAVNNEPNYEGDAENAPNFFSGLAST
LGYLRKKSVFTTGDVDLKPGKDVNNSESLRRDVRNKEHTGTGTYTKDKLHGLEQFTSS
DSSNANTHSKRQDHYDPDIKLVYRDEKGNRLTTKEAYKKLSQKFHGTKSNKKKRAKMK
SRIEARKNTPENGSLFEFDDN"
gene complement(<896825..>898360)
/gene="NOP58"
/locus_tag="YOR310C"
/gene_synonym="NOP5"
/db_xref="GeneID:854487"
mRNA complement(<896825..>898360)
/gene="NOP58"
/locus_tag="YOR310C"
/gene_synonym="NOP5"
/product="RNA-processing protein NOP58"
/transcript_id="NM_001183730.1"
/db_xref="GeneID:854487"
CDS complement(896825..898360)
/gene="NOP58"
/locus_tag="YOR310C"
/gene_synonym="NOP5"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:9372940]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/experiment="EXISTENCE:direct assay:GO:0031428 box C/D
methylation guide snoRNP complex
[PMID:11081632|PMID:10094313]"
/experiment="EXISTENCE:direct assay:GO:0032040
small-subunit processome [PMID:12068309]"
/experiment="EXISTENCE:mutant phenotype:GO:0000447
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from
5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10094313]"
/experiment="EXISTENCE:mutant phenotype:GO:0000472
endonucleolytic cleavage to generate mature 5'-end of
SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:10094313]"
/experiment="EXISTENCE:mutant phenotype:GO:0000480
endonucleolytic cleavage in 5'-ETS of tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:10094313]"
/experiment="EXISTENCE:physical interaction:GO:0031428 box
C/D methylation guide snoRNP complex [PMID:10733567]"
/note="Protein involved in producing mature rRNAs and
snoRNAs; involved in pre-rRNA processing, 18S rRNA
synthesis, and snoRNA synthesis; component of the small
subunit processome complex, which is required for
processing of pre-18S rRNA"
/codon_start=1
/product="RNA-processing protein NOP58"
/protein_id="NP_014955.1"
/db_xref="GeneID:854487"
/db_xref="SGD:S000005837"
/translation="MAYVLTETSAGYALLKASDKKIYKSSSLIQDLDSSDKVLKEFKI
AAFSKFNSAANALEEANSIIEGKVSSQLEKLLEEIKKDKKSTLIVSETKLANAINKLG
LNFNVVSDAVTLDIYRAIKEYLPELLPGMSDNDLSKMSLGLAHSIGRHKLKFSADKVD
VMIIQAIALLDDLDKELNTYAMRCKEWYGWHFPELAKIVTDSVAYARIILTMGIRSKA
SETDLSEILPEEIEERVKTAAEVSMGTEITQTDLDNINALAEQIVEFAAYREQLSNYL
SARMKAIAPNLTQLVGELVGARLIAHSGSLISLAKSPASTIQILGAEKALFRALKTKH
DTPKYGLLYHASLVGQATGKNKGKIARVLAAKAAVSLRYDALAEDRDDSGDIGLESRA
KVENRLSQLEGRDLRTTPKVVREAKKVEMTEARAYNADADTAKAASDSESDSDDEEEE
KKEKKEKKRKRDDDEDSKDSKKAKKEKKDKKEKKEKKEKKEKKEKKEKKEKKSKKEKK
EKK"
gene complement(<899056..>899928)
/gene="DGK1"
/locus_tag="YOR311C"
/gene_synonym="HSD1"
/db_xref="GeneID:854488"
mRNA complement(<899056..>899928)
/gene="DGK1"
/locus_tag="YOR311C"
/gene_synonym="HSD1"
/product="diacylglycerol kinase"
/transcript_id="NM_001183731.3"
/db_xref="GeneID:854488"
CDS complement(899056..899928)
/gene="DGK1"
/locus_tag="YOR311C"
/gene_synonym="HSD1"
/EC_number="2.7.1.174"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:11481671]"
/experiment="EXISTENCE:direct assay:GO:0006654
phosphatidic acid biosynthetic process [PMID:18458076]"
/experiment="EXISTENCE:direct assay:GO:0141035
CTP-dependent diacylglycerol kinase activity
[PMID:18458076|PMID:18458075]"
/experiment="EXISTENCE:mutant phenotype:GO:0006654
phosphatidic acid biosynthetic process [PMID:18458075]"
/experiment="EXISTENCE:mutant phenotype:GO:2001210
regulation of isopentenyl diphosphate biosynthetic
process, mevalonate pathway [PMID:32058036]"
/note="Diacylglycerol kinase; localized to the endoplasmic
reticulum (ER); overproduction induces enlargement of
ER-like membrane structures, stimulates the
mevalonate/ergosterol pathway and suppresses a
temperature-sensitive sly1 mutation; contains a CTP
transferase domain"
/codon_start=1
/product="diacylglycerol kinase"
/protein_id="NP_014956.3"
/db_xref="GeneID:854488"
/db_xref="SGD:S000005838"
/translation="MGTEDAIALPNSTLEPRTEAKQRLSSKSHQVSAKVTIPAKEEIS
SSDDDAHVPVTEIHLKSHEWFGDFITKHEIPRKVFHSSIGFITLYLYTQGINYKNVLW
PLIYAFIILFILDLIRLNWPFFNMLYCRTVGALMRKKEIHTYNGVLWYILGLIFSFNF
FSKDVTLISLFLLSWSDTAAATIGRKYGHLTPKVARNKSLAGSIAAFTVGVITCWVFY
GYFVPAYSYVNKPGEIQWSPETSRLSLNMLSLLGGVVAALSEGIDLFNWDDNFTIPVL
SSLFMNAVIKTFKK"
gene complement(<900250..>901194)
/gene="RPL20B"
/locus_tag="YOR312C"
/gene_synonym="RPL18A1"
/db_xref="GeneID:854489"
mRNA complement(join(<900250..900767,901194..>901194))
/gene="RPL20B"
/locus_tag="YOR312C"
/gene_synonym="RPL18A1"
/product="60S ribosomal protein eL20 RPL20B"
/transcript_id="NM_001183732.1"
/db_xref="GeneID:854489"
CDS complement(join(900250..900767,901194))
/gene="RPL20B"
/locus_tag="YOR312C"
/gene_synonym="RPL18A1"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:23874617]"
/experiment="EXISTENCE:curator inference:GO:0003735
structural constituent of ribosome [PMID:23874617]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:23874617]"
/note="Ribosomal 60S subunit protein L20B; homologous to
mammalian ribosomal protein L18A, no bacterial homolog;
RPL20B has a paralog, RPL20A, that arose from the whole
genome duplication"
/codon_start=1
/product="60S ribosomal protein eL20 RPL20B"
/protein_id="NP_014957.2"
/db_xref="GeneID:854489"
/db_xref="SGD:S000005839"
/translation="MAHFKEYQVIGRRLPTESVPEPKLFRMRIFASNEVIAKSRYWYF
LQKLHKVKKASGEIVSINQINEAHPTKVKNFGVWVRYDSRSGTHNMYKEIRDVSRVAA
VETLYQDMAARHRARFRSIHILKVAEIEKTADVKRQYVKQFLTKDLKFPLPHRVQKST
KTFSYKRPSTFY"
gene complement(<901858..>902874)
/gene="SPS4"
/locus_tag="YOR313C"
/db_xref="GeneID:854490"
mRNA complement(<901858..>902874)
/gene="SPS4"
/locus_tag="YOR313C"
/product="Sps4p"
/transcript_id="NM_001183733.3"
/db_xref="GeneID:854490"
CDS complement(901858..902874)
/gene="SPS4"
/locus_tag="YOR313C"
/experiment="EXISTENCE:direct assay:GO:0005811 lipid
droplet [PMID:24390141]"
/experiment="EXISTENCE:expression pattern:GO:0030437
ascospore formation [PMID:3540611]"
/note="Protein whose expression is induced during
sporulation; not required for sporulation; heterologous
expression in E. coli induces the SOS response that senses
DNA damage"
/codon_start=1
/product="Sps4p"
/protein_id="NP_014958.3"
/db_xref="GeneID:854490"
/db_xref="SGD:S000005840"
/translation="MPSNLNIVKVTKPQEENKNFLHKNTNEPNEMEQSQTQEAVTENF
TENSNLSANEHAARRGRLNLKTVDHIETYPIVQETEEIAKKIALTRIILAQTKPRIDK
VVVSRPVQAVAPVVNFFDKMANSTLSTVERVVPSLKTKTYKRLGEEIALPYTLSKKYG
KQLRDTTARNGDNYVYQPVHGRLMKFRKYYNEKFIDTKGKPLIRGQLDPVLLPVNNTF
EKVTVKYLPKGKKVPNDSFSCEFNRGLALEYNFMTRAVSAVSHQVVGIAKLPIAYGYH
TNSVYNKNLDKQADLKMKNVLRGTWDTITDLEREIWASVTDRSLFRFFGNKSEGGDLP
HLVQ"
gene <903043..>903372
/locus_tag="YOR314W"
/db_xref="GeneID:854491"
mRNA <903043..>903372
/locus_tag="YOR314W"
/product="uncharacterized protein"
/transcript_id="NM_001348880.1"
/db_xref="GeneID:854491"
CDS 903043..903372
/locus_tag="YOR314W"
/note="hypothetical protein; conserved across S.
cerevisiae strains"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335818.1"
/db_xref="GeneID:854491"
/db_xref="SGD:S000005841"
/translation="MTMIRFCGARQSAIISNASDAAAGTNKKRILNPLESLCLNDRID
EHRCKEVQLSSLRSLLYAMILNRTIGSETGVFSFLLFSFRYFGEERDLFYCFFSVFLL
NITYLLD"
gene <904760..>905800
/gene="SFG1"
/locus_tag="YOR315W"
/db_xref="GeneID:854493"
mRNA <904760..>905800
/gene="SFG1"
/locus_tag="YOR315W"
/product="Sfg1p"
/transcript_id="NM_001183735.1"
/db_xref="GeneID:854493"
CDS 904760..905800
/gene="SFG1"
/locus_tag="YOR315W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:16289536|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0007124
pseudohyphal growth [PMID:16289536]"
/experiment="EXISTENCE:mutant phenotype:GO:0000278 mitotic
cell cycle [PMID:19033152]"
/experiment="EXISTENCE:mutant phenotype:GO:0007124
pseudohyphal growth [PMID:16289536]"
/experiment="EXISTENCE:mutant phenotype:GO:0036267
invasive filamentous growth [PMID:32665277]"
/experiment="EXISTENCE:mutant phenotype:GO:0090033
positive regulation of filamentous growth [PMID:32665277]"
/experiment="EXISTENCE:mutant phenotype:GO:0098609
cell-cell adhesion [PMID:32665277]"
/note="Putative transcription factor; induces superficial
pseudohyphal growth, positively regulates invasive growth,
but is not required for invasive pseudohyphal growth; may
act together with Phd1p; promotes cell adhesion
independent of Flo11p by repressing genes that encode cell
wall degrading enzymes; localizes to the nucleus;
potential Cdc28p substrate"
/codon_start=1
/product="Sfg1p"
/protein_id="NP_014960.1"
/db_xref="GeneID:854493"
/db_xref="SGD:S000005842"
/translation="MDEMHSSDTLLLRTPKSKKKIGLVIPSTPSKKCKYSSGFIAEDT
TPSKRFRLYQAKFKTSSKNVKAQTLSVSIKKNQGEITNPFMTEGYNDYRNIVSPGLSF
DNDCFSEHELVSPLSDISSINSTSPDVEKIDSLDPFGVDSFVWNCKPLVNKEALELHR
MIHSSFPMSPLESNSDVPLLLPKLKKRLSPVNRSTFKPTRYEPSHRLLKPKKSILTVP
AKSLNLIVSSSRGSLNDATIFATEINSTLSNEENKLPAISSIWEKLTIPVNSSIKEKY
KKLKDQIYGQASNFGEDEDNEEDNEDDLPDAAVIRGYEFQSGRRDELTQCNELQSTKD
YKKVQWAKVLEQ"
gene complement(<906236..>907555)
/gene="COT1"
/locus_tag="YOR316C"
/db_xref="GeneID:854494"
mRNA complement(<906236..>907555)
/gene="COT1"
/locus_tag="YOR316C"
/product="metal cation transporter COT1"
/transcript_id="NM_001183736.3"
/db_xref="GeneID:854494"
CDS complement(906236..907555)
/gene="COT1"
/locus_tag="YOR316C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:9712830]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane
[PMID:23836928|PMID:27881666|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0045121 membrane
raft [PMID:23836928]"
/experiment="EXISTENCE:genetic interaction:GO:0005385 zinc
ion transmembrane transporter activity [PMID:8058041]"
/experiment="EXISTENCE:genetic interaction:GO:0006829 zinc
ion transport [PMID:8058041]"
/experiment="EXISTENCE:mutant phenotype:GO:0006824 cobalt
ion transport [PMID:1508175]"
/experiment="EXISTENCE:mutant phenotype:GO:0006882
intracellular zinc ion homeostasis [PMID:10856230]"
/experiment="EXISTENCE:mutant phenotype:GO:0015087 cobalt
ion transmembrane transporter activity [PMID:1508175]"
/note="Vacuolar transporter that mediates zinc transport
into vacuole; overexpression confers resistance to cobalt
and rhodium; targeted to vacuole via AP-3 pathway; protein
abundance increases in response to DNA replication stress;
COT1 has a paralog, ZRC1, that arose from the whole genome
duplication"
/codon_start=1
/product="metal cation transporter COT1"
/protein_id="NP_014961.3"
/db_xref="GeneID:854494"
/db_xref="SGD:S000005843"
/translation="MKLGSKQVKIISLLLLDTVFFGIEITTGYLSHSLALIADSFHML
NDIISLVVALWAVNVAKNRNPDSTYTYGWKRAEILGALINAVFLIALCVSILIEALQR
IIAPPVIENPKFVLYVGVAGLISNTVGLFLFHDNDQEHGHGHGHSHGGIFADHEMHMP
SSHTHTHAHVDGIENTTPMDSTDNISEIMPNAIVDSFMNENTRLLTPENASKTPSYST
SSHTIASGGNYTEHNKRKRSLNMHGVFLHVLGDALGNIGVMLSAFFIWKTDYSWKYYT
DPLVSLIITGIIFSSALPLSCKASKILLQATPSTLSGDQVEGDLLKIPGIIAIHDFHI
WNLTESIFIASLHIQLDISPEQFTDLAKIVRSKLHRYGIHSATLQPEFITREVTSTER
AGDSQGDHLQNDPLSLRPKTYGTGISGSTCLIDDAANCNTADCLEDH"
gene complement(<907726..>907935)
/locus_tag="YOR316C-A"
/db_xref="GeneID:1466487"
mRNA complement(<907726..>907935)
/locus_tag="YOR316C-A"
/product="uncharacterized protein"
/transcript_id="NM_001184577.1"
/db_xref="GeneID:1466487"
CDS complement(907726..907935)
/locus_tag="YOR316C-A"
/note="hypothetical protein; identified by fungal homology
and RT-PCR"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878175.1"
/db_xref="GeneID:1466487"
/db_xref="SGD:S000028584"
/translation="MLVPMHNSPTAANGRLSLTVASSGLRKGKKNRVYTIHSYIRSPV
SSSEFSFSVRRQYKLTIRIKQKTHL"
rep_origin 908296..908544
/note="ARS1528; Autonomously Replicating Sequence"
/db_xref="SGD:S000118495"
gene <909343..>911445
/gene="FAA1"
/locus_tag="YOR317W"
/db_xref="GeneID:854495"
mRNA <909343..>911445
/gene="FAA1"
/locus_tag="YOR317W"
/product="long-chain fatty acid-CoA ligase FAA1"
/transcript_id="NM_001183737.3"
/db_xref="GeneID:854495"
CDS 909343..911445
/gene="FAA1"
/locus_tag="YOR317W"
/EC_number="6.2.1.3"
/experiment="EXISTENCE:direct assay:GO:0004467 long-chain
fatty acid-CoA ligase activity [PMID:8206942]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:16407407]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005811 lipid
droplet [PMID:24868093|PMID:10515935]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836|PMID:27136724]"
/experiment="EXISTENCE:direct assay:GO:0031956
medium-chain fatty acid-CoA ligase activity
[PMID:8206942]"
/experiment="EXISTENCE:genetic interaction:GO:0004467
long-chain fatty acid-CoA ligase activity
[PMID:11477098|PMID:22633490]"
/experiment="EXISTENCE:genetic interaction:GO:0035336
long-chain fatty-acyl-CoA metabolic process
[PMID:11477098]"
/experiment="EXISTENCE:genetic interaction:GO:0044539
long-chain fatty acid import into cell
[PMID:27136724|PMID:12601005]"
/experiment="EXISTENCE:genetic interaction:GO:1905329
sphingoid long-chain base transport [PMID:27136724]"
/experiment="EXISTENCE:mutant phenotype:GO:0004467
long-chain fatty acid-CoA ligase activity [PMID:11477098]"
/experiment="EXISTENCE:mutant phenotype:GO:0035336
long-chain fatty-acyl-CoA metabolic process
[PMID:11477098]"
/experiment="EXISTENCE:mutant phenotype:GO:0044539
long-chain fatty acid import into cell
[PMID:27136724|PMID:12601005]"
/experiment="EXISTENCE:mutant phenotype:GO:1905329
sphingoid long-chain base transport [PMID:27136724]"
/note="Long chain fatty acyl-CoA synthetase; activates
fatty acids with a preference for C12:0-C16:0 chain
lengths; role in the competitive import of long-chain
fatty acids and sphingoid long-chain bases; accounts for
most acyl-CoA synthetase activity; localizes to lipid
particles and the plasma membrane; role in
sphingolipid-to-glycerolipid metabolism; forms ER foci
upon replication stress; faa1 faa4 double null
complemented by any of human ACSBG1, ACSL1, 3, 4, 5, 6,
SLC27A2, or 4"
/codon_start=1
/product="long-chain fatty acid-CoA ligase FAA1"
/protein_id="NP_014962.3"
/db_xref="GeneID:854495"
/db_xref="SGD:S000005844"
/translation="MVAQYTVPVGKAANEHETAPRRNYQCREKPLVRPPNTKCSTVYE
FVLECFQKNKNSNAMGWRDVKEIHEESKSVMKKVDGKETSVEKKWMYYELSHYHYNSF
DQLTDIMHEIGRGLVKIGLKPNDDDKLHLYAATSHKWMKMFLGAQSQGIPVVTAYDTL
GEKGLIHSLVQTGSKAIFTDNSLLPSLIKPVQAAQDVKYIIHFDSISSEDRRQSGKIY
QSAHDAINRIKEVRPDIKTFSFDDILKLGKESCNEIDVHPPGKDDLCCIMYTSGSTGE
PKGVVLKHSNVVAGVGGASLNVLKFVGNTDRVICFLPLAHIFELVFELLSFYWGACIG
YATVKTLTSSSVRNCQGDLQEFKPTIMVGVAAVWETVRKGILNQIDNLPFLTKKIFWT
AYNTKLNMQRLHIPGGGALGNLVFKKIRTATGGQLRYLLNGGSPISRDAQEFITNLIC
PMLIGYGLTETCASTTILDPANFELGVAGDLTGCVTVKLVDVEELGYFAKNNQGEVWI
TGANVTPEYYKNEEETSQALTSDGWFKTGDIGEWEANGHLKIIDRKKNLVKTMNGEYI
ALEKLESVYRSNEYVANICVYADQSKTKPVGIIVPNHAPLTKLAKKLGIMEQKDSSIN
IENYLEDAKLIKAVYSDLLKTGKDQGLVGIELLAGIVFFDGEWTPQNGFVTSAQKLKR
KDILNAVKDKVDAVYSSS"
gene <912822..>913463
/gene="HSH49"
/locus_tag="YOR319W"
/db_xref="GeneID:854497"
mRNA <912822..>913463
/gene="HSH49"
/locus_tag="YOR319W"
/product="U2 snRNP complex subunit HSH49"
/transcript_id="NM_001183739.1"
/db_xref="GeneID:854497"
CDS 912822..913463
/gene="HSH49"
/locus_tag="YOR319W"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:9436903]"
/experiment="EXISTENCE:direct assay:GO:0005686 U2 snRNP
[PMID:16314500|PMID:15565172]"
/experiment="EXISTENCE:direct assay:GO:0071004 U2-type
prespliceosome [PMID:16618970]"
/experiment="EXISTENCE:mutant phenotype:GO:0000398 mRNA
splicing, via spliceosome [PMID:9436903]"
/experiment="EXISTENCE:physical interaction:GO:0000398
mRNA splicing, via spliceosome [PMID:16314500]"
/note="U2-snRNP associated splicing factor; similar to the
mammalian splicing factor SAP49; proposed to function as a
U2-snRNP assembly factor along with Hsh155p and binding
partner Cus1p; contains two RNA recognition motifs (RRM)"
/codon_start=1
/product="U2 snRNP complex subunit HSH49"
/protein_id="NP_014964.1"
/db_xref="GeneID:854497"
/db_xref="SGD:S000005846"
/translation="MNYSADSGNTVYVGNIDPRITKEQLYELFIQINPVLRIKYPKDK
VLQAYQGYAFIEFYNQGDAQYAIKIMNNTVRLYDRLIKVRQVTNSTGTTNLPSNISKD
MILPIAKLFIKNLADSIDSDQLVKIFNKFGKLIREPEIFYLSNGKLKCAYVYFEDFEK
ADLAIKSLNNQLVANNRITVDYAFKENGKGNAKYGDDVDRLLNKEALKHNMLK"
gene complement(<913619..>915094)
/gene="GNT1"
/locus_tag="YOR320C"
/db_xref="GeneID:854498"
mRNA complement(<913619..>915094)
/gene="GNT1"
/locus_tag="YOR320C"
/product="glucose N-acetyltransferase"
/transcript_id="NM_001183740.3"
/db_xref="GeneID:854498"
CDS complement(913619..915094)
/gene="GNT1"
/locus_tag="YOR320C"
/experiment="EXISTENCE:direct assay:GO:0005797 Golgi
medial cisterna [PMID:12651885]"
/experiment="EXISTENCE:direct assay:GO:0008375
acetylglucosaminyltransferase activity [PMID:12651885]"
/experiment="EXISTENCE:mutant phenotype:GO:0006487 protein
N-linked glycosylation [PMID:12651885]"
/note="N-acetylglucosaminyltransferase; capable of
modification of N-linked glycans in the Golgi apparatus"
/codon_start=1
/product="glucose N-acetyltransferase"
/protein_id="NP_014965.3"
/db_xref="GeneID:854498"
/db_xref="SGD:S000005847"
/translation="MRLISKRRIRFIVFILFGVLTVFVVSRLVVHFQYNQEIKFYKKY
FQQRKDGLHEIYNPLEIKQIPKETIDDLYTARLDKELKNGEVIEWSKFAYVNYVTNAD
YLCNTLIIFNDLKQEFETKAKLVLLISKDLLDPNTSSNVAYISSLLNKIQAIDEDQVV
IKLIDNIVKPKDTTPWNESLTKLLVFNQTEFDRVIYLDNDAILRSSLDELFFLPNYIK
FAAPLTYWFLSNSDLEKSYHETRHREKQPINLQSYTKVLTKRIGKGQMIYNHLPSLPH
SLYLNSNNIAQDIISSTSSLSPLFDFQSSKKVGKLKFASNLMVINPSKEAFDEIVNVM
LPKILNKKEKYDMDLINEEMYNLKKIIYKQFIFFRKVRKLFKPEVLVLPFARYGLLTG
SLRNPRHYSIIYNDVLGYKTLDNDGNDIPVGLNDSVAYSKYIHFSDYPLAKPWNYPSM
KEFECIVKEEDAEDSKLEHQACDLWNSVYASYIQSREICLV"
gene <916030..>918291
/gene="PMT3"
/locus_tag="YOR321W"
/db_xref="GeneID:854499"
mRNA <916030..>918291
/gene="PMT3"
/locus_tag="YOR321W"
/product="dolichyl-phosphate-mannose-protein
mannosyltransferase PMT3"
/transcript_id="NM_001183741.1"
/db_xref="GeneID:854499"
CDS 916030..918291
/gene="PMT3"
/locus_tag="YOR321W"
/EC_number="2.4.1.109"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0097583
dolichyl-phosphate-mannose-protein mannosyltransferase
Pmt1p-Pmt3p dimer complex [PMID:12551906]"
/experiment="EXISTENCE:direct assay:GO:0097585
dolichyl-phosphate-mannose-protein mannosyltransferase
Pmt5p-Pmt3p dimer complex [PMID:12551906]"
/experiment="EXISTENCE:genetic interaction:GO:0035269
protein O-linked glycosylation via mannose [PMID:9184828]"
/note="Protein O-mannosyltransferase; transfers mannose
residues from dolichyl phosphate-D-mannose to protein
serine/threonine residues; acts in a complex with Pmt5p,
can instead interact with Pmt1p in some conditions;
antifungal drug target; PMT3 has a paralog, PMT2, that
arose from the whole genome duplication"
/codon_start=1
/product="dolichyl-phosphate-mannose-protein
mannosyltransferase PMT3"
/protein_id="NP_014966.1"
/db_xref="GeneID:854499"
/db_xref="SGD:S000005848"
/translation="MPYRVATGYSEKSTDDDLIWRTPIVKEELEDADNFLKDDAELYD
KVKNESAVSHLDTIVMPIIFTVLGMFTRMYKIGRNNHVVWDEAHFGKFGSYYLRHEFY
HDVHPPLGKMLVGLSGYLAGYNGSWDFPSGEVYPDYIDYVKMRLFQAMFSSLCVPLAY
FTGRAIGFSRLSVWLFTILVIFENSYATLGKFILLDSMLLFFTVSSYFCLAKFHTMRK
SPFSARWWLWLCLTGLNLGCAISVKMVGLFIISVVGIYTISELWNLLSDRSVSWKVYV
NHWLARIFGLIIIPVCVFLLCFKIHFDLLSNSGPGDSTMPSLFQASLNGTKVGKGPRD
VALGSSIISIKNQALGGALLHSHVQPFPEGSEQQQVTVYGYSDANNEWFFQRIRGVEP
WTDAENKTIEFVKGGEMYRLMHRLTGKNLHTHEVPAPISKSEYEVSAYGDVDLGDYKD
NWIIEIVEQVGEEDPTLLHPLSTSFRIKNSILGCYLAQSGKHLPEWGFRQGEVVCLKH
ASKRDKRTWWNIETHENERLPQGEDFVYPKTSFFRNFMQLNSAMMATNNALVPNPEKF
DGIASSAWQWPTLNVGVRLCEWSEKSIKYFLLGSPASVWPSSIAVCALIIHVIFLTLK
WQRQCVILSDPVERDVFVMAAFYPLLAWLLHYMPFVVMSRVVYAHHYLPTLYFALMIL
SYYFDMITKRWATRNTGKFLRLGAYIVYGSIVIAGFFYFSPFSFGMDGPVDDYAYLAW
LPTWQIVEDIRNT"
gene complement(<918606..>921062)
/gene="LDB19"
/locus_tag="YOR322C"
/gene_synonym="ART1"
/db_xref="GeneID:854500"
mRNA complement(<918606..>921062)
/gene="LDB19"
/locus_tag="YOR322C"
/gene_synonym="ART1"
/product="Ldb19p"
/transcript_id="NM_001183742.3"
/db_xref="GeneID:854500"
CDS complement(918606..921062)
/gene="LDB19"
/locus_tag="YOR322C"
/gene_synonym="ART1"
/experiment="EXISTENCE:direct assay:GO:0000138 Golgi trans
cisterna [PMID:18976803]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:18976803|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:22118465]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762|PMID:22118465]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:22118465|PMID:18976803]"
/experiment="EXISTENCE:direct assay:GO:0031625 ubiquitin
protein ligase binding [PMID:24820415]"
/experiment="EXISTENCE:genetic interaction:GO:0002092
positive regulation of receptor internalization
[PMID:24820415]"
/experiment="EXISTENCE:mutant phenotype:GO:0002092
positive regulation of receptor internalization
[PMID:24820415]"
/experiment="EXISTENCE:mutant phenotype:GO:0031625
ubiquitin protein ligase binding [PMID:24820415]"
/experiment="EXISTENCE:mutant phenotype:GO:0033554
cellular response to stress [PMID:18976803]"
/experiment="EXISTENCE:mutant phenotype:GO:0070086
ubiquitin-dependent endocytosis
[PMID:18976803|PMID:32744498]"
/experiment="EXISTENCE:mutant phenotype:GO:0071230
cellular response to amino acid stimulus [PMID:18976803]"
/experiment="EXISTENCE:physical interaction:GO:0031625
ubiquitin protein ligase binding [PMID:18976803]"
/experiment="EXISTENCE:physical interaction:GO:0070086
ubiquitin-dependent endocytosis [PMID:18976803]"
/note="Alpha-arrestin, Ub-ligase adaptor for Rsp5p;
regulates starvation- and substrate-induced Ub-dependent
endocytosis of select plasma membrane localized amino acid
transporters, recruiting Rsp5p to its targets; role in
basal internalization and turnover of Ste2p, mating and
zygote formation; recruits Rsp5p to Ste2p via its 2 PPXY
motifs; inhibited by Npr1p-mediated phosphorylation,
affecting cytosol and plasma membrane translocation;
localization regulated by Rsp5p-dependent ubiquitination"
/codon_start=1
/product="Ldb19p"
/protein_id="NP_014967.3"
/db_xref="GeneID:854500"
/db_xref="SGD:S000005849"
/translation="MAFSRLTSTHQSNHNGYSNSNKKGQSLPLTLSIDVESPPCVLYG
SAMESSGAVLSGLFTVTVVDPYSSAEDKSLKNTESNVSTTSKSLKRKSTFGSALSSRL
SSLSASTSNISPSTSSTSISHSPTPANLRIMAGYTKITITSVTLSLVQKIHFHKPFVP
NISSMQTCMNCKTKITNMKSWEIQSNTQDLSVGSHSYPFSYLIPGSVPCSSSLGATAE
TQVKYELIAVVTYIDPHRNSFSSGHSTPRKEGSSSKKRLLQLAMPIAVTRSIPRGPDK
NSLRVFPPTELTAAAVLPNVVYPKSTFPLEMKLDGVSSGDRRWRMRKLSWRIEETTRV
KAHACPVHKHELRQLEEQVKIKESEKSKKPRSHIKRYGELGPQIRVAVNSLENMPSQR
LPGEPGREQAPNSSGPASTGNVGLDDENPVNEDEEDQPGSEFIHPSDDALRQELLMQQ
QRARQQQLQQELKNNSSLFTEEVRIISKGEMKSGWKTDFDNNGKIELVTEIDCMGLNS
GVSNPVMHASTLQTPSTGNKKPSINVACDIQDPNLGLYVSHILAVEIVVAEETLQYAN
GQPIRKPNSKNKKETNNNTMNVHNPDQRLAELSPIFANRNTPKVRRMGPEDITPVNSN
KSNHSTNKEKASNGASNSNIVSVPTGAARVLRMQFRLTVTERSGLGISWDEEVPPIYQ
DVELLSPPCYELSINNGIKNKLYSTMSTPVRSEDDFVGGSDEDIGNYESQGLEPGPNV
QEVTITQNKLTIPPTAHHYQPASSSQRSLTTVQSPPLESVVSVQGSVPFRGHVLTPHS
TRDIRIQNFSDFLDSNRITQ"
gene complement(<921535..>922905)
/gene="PRO2"
/locus_tag="YOR323C"
/db_xref="GeneID:854501"
mRNA complement(<921535..>922905)
/gene="PRO2"
/locus_tag="YOR323C"
/product="glutamate-5-semialdehyde dehydrogenase"
/transcript_id="NM_001183743.1"
/db_xref="GeneID:854501"
CDS complement(921535..922905)
/gene="PRO2"
/locus_tag="YOR323C"
/EC_number="1.2.1.41"
/experiment="EXISTENCE:direct assay:GO:0004350
glutamate-5-semialdehyde dehydrogenase activity
[PMID:12513997]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:25112878]"
/experiment="EXISTENCE:direct assay:GO:0055129 L-proline
biosynthetic process [PMID:12513997]"
/note="Cytosolic gamma-glutamyl phosphate reductase;
catalyzes the second step in proline biosynthesis"
/codon_start=1
/product="glutamate-5-semialdehyde dehydrogenase"
/protein_id="NP_014968.1"
/db_xref="GeneID:854501"
/db_xref="SGD:S000005850"
/translation="MSSSQQIAKNARKAGNILKTISNEGRSDILYKIHDALKANAHAI
EEANKIDLAVAKETGLADSLLKRLDLFKGDKFEVMLQGIKDVAELEDPVGKVKMAREL
DDGLTLYQVTAPVGVLLVIFESRPEVIANITALSIKSGNAAILKGGKESVNTFREMAK
IVNDTIAQFQSETGVPVGSVQLIETRQDVSDLLDQDEYIDLVVPRGSNALVRKIKDTT
KIPVLGHADGICSIYLDEDADLIKAKRISLDAKTNYPAGCNAMETLLINPKFSKWWEV
LENLTLEGGVTIHATKDLKTAYFDKLNELGKLTEAIQCKTVDADEEQDFDKEFLSLDL
AAKFVTSTESAIQHINTHSSRHTDAIVTENKANAEKFMKGVDSSGVYWNASTRFADGF
RYGFGAEVGISTSKIHARGPVGLDGLVSYQYQIRGDGQVASDYLGAGGNKAFVHKDLD
IKTVTL"
gene complement(<923232..>925040)
/gene="FRT1"
/locus_tag="YOR324C"
/gene_synonym="HPH1"
/db_xref="GeneID:854503"
mRNA complement(<923232..>925040)
/gene="FRT1"
/locus_tag="YOR324C"
/gene_synonym="HPH1"
/product="Frt1p"
/transcript_id="NM_001183744.3"
/db_xref="GeneID:854503"
CDS complement(923232..925040)
/gene="FRT1"
/locus_tag="YOR324C"
/gene_synonym="HPH1"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762|PMID:12514182|PMID:15189990]"
/experiment="EXISTENCE:mutant phenotype:GO:0071469
cellular response to alkaline pH [PMID:15189990]"
/experiment="EXISTENCE:mutant phenotype:GO:0071472
cellular response to salt stress [PMID:15189990]"
/experiment="EXISTENCE:physical interaction:GO:0031204
post-translational protein targeting to membrane,
translocation [PMID:21097665]"
/note="Tail-anchored ER membrane hypothetical protein;
substrate of the phosphatase calcineurin; interacts with
homolog Frt2p; promotes cell growth in stress conditions,
possibly via a role in posttranslational translocation;
FRT1 has a paralog, FRT2, that arose from the whole genome
duplication"
/codon_start=1
/product="Frt1p"
/protein_id="NP_014969.3"
/db_xref="GeneID:854503"
/db_xref="SGD:S000005851"
/translation="MNLLIDRMENPGSRNCTLLPPSFPRGFCKGRRASSGDAVKIKES
GLQPQPQPEPLQAKTNVAHFSKSSSRLPVIAVNDNPVVPRPSTEVNLGSLLQKEREKE
KEEQPALHDRRHLYVTKNRAHGVRQRSLEMTSLPVLGSTKTGKFSDFLFEDDIDNRVG
RHSRSYSGASSLDDPFRVSPKTDFNSNRARLSCLSKGRRGSMSVFQSCHTGLAFNQIQ
GSSSSQRRSSAGSFDYERKRLVNQFLQPSLGNSDPFDTLRESVVFEPSSTAGGIKLGN
MHSQSQISVNSSPSTSLFYHDLDGSAVNDSSSFLYSRSNVPAFLSSSAFSSTSSTSSD
SEDVDRRSLNGVYPSLGYLTNQRKPRNSSGSSTAPGTDTLGFKYLLNRQKSADSSTRF
KSVLKVNNNNGSAATPDSSSNSISKSNSNLNDNIDELNYYQNHISTLLVKIENEMRRN
LNDTIIKNENNVQKTIQKYDLLSGELTLLLDEMTTLRTTVINQFLVKLKSDFDEDDNK
AFINELKISVEESVAQLQGLERRMEVCQERLNKQKSSLREMDSLIELKNVLNKSKNNT
KSIYLYRYFIIDIIAFLLMGGFIVYVKNLLTRFFTR"
gene <925721..>930445
/gene="MYO2"
/locus_tag="YOR326W"
/gene_synonym="CDC66"
/db_xref="GeneID:854504"
mRNA <925721..>930445
/gene="MYO2"
/locus_tag="YOR326W"
/gene_synonym="CDC66"
/product="myosin 2"
/transcript_id="NM_001183746.1"
/db_xref="GeneID:854504"
CDS 925721..930445
/gene="MYO2"
/locus_tag="YOR326W"
/gene_synonym="CDC66"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:8188749]"
/experiment="EXISTENCE:direct assay:GO:0000146
microfilament motor activity [PMID:11381095]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:8978821]"
/experiment="EXISTENCE:direct assay:GO:0005516 calmodulin
binding [PMID:8294515]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:8188749]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:8188749]"
/experiment="EXISTENCE:direct assay:GO:0030133 transport
vesicle [PMID:23079598]"
/experiment="EXISTENCE:direct assay:GO:0031941 filamentous
actin [PMID:11381095]"
/experiment="EXISTENCE:direct assay:GO:0031982 vesicle
[PMID:12456647]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807|PMID:8188749]"
/experiment="EXISTENCE:direct assay:GO:0051015 actin
filament binding [PMID:11381095]"
/experiment="EXISTENCE:genetic interaction:GO:0000001
mitochondrion inheritance [PMID:12391144]"
/experiment="EXISTENCE:genetic interaction:GO:0000011
vacuole inheritance [PMID:12594460]"
/experiment="EXISTENCE:genetic interaction:GO:0006904
vesicle docking involved in exocytosis [PMID:27307583]"
/experiment="EXISTENCE:genetic interaction:GO:0007107
membrane addition at site of cytokinesis [PMID:12456647]"
/experiment="EXISTENCE:genetic interaction:GO:0048312
intracellular distribution of mitochondria
[PMID:12391144|PMID:15201867]"
/experiment="EXISTENCE:mutant phenotype:GO:0000001
mitochondrion inheritance [PMID:12391144]"
/experiment="EXISTENCE:mutant phenotype:GO:0000011 vacuole
inheritance [PMID:8978821]"
/experiment="EXISTENCE:mutant phenotype:GO:0000132
establishment of mitotic spindle orientation
[PMID:10984058]"
/experiment="EXISTENCE:mutant phenotype:GO:0006898
receptor-mediated endocytosis [PMID:37647159]"
/experiment="EXISTENCE:mutant phenotype:GO:0007107
membrane addition at site of cytokinesis [PMID:12456647]"
/experiment="EXISTENCE:mutant phenotype:GO:0016192
vesicle-mediated transport [PMID:2016335]"
/experiment="EXISTENCE:mutant phenotype:GO:0030050 vesicle
transport along actin filament
[PMID:10562281|PMID:9864365]"
/experiment="EXISTENCE:mutant phenotype:GO:0032432 actin
filament bundle [PMID:9864365]"
/experiment="EXISTENCE:mutant phenotype:GO:0034993 meiotic
nuclear membrane microtubule tethering complex
[PMID:32059771]"
/experiment="EXISTENCE:mutant phenotype:GO:0045033
peroxisome inheritance [PMID:11733545]"
/experiment="EXISTENCE:mutant phenotype:GO:0048312
intracellular distribution of mitochondria
[PMID:15201867|PMID:12391144]"
/experiment="EXISTENCE:mutant phenotype:GO:0048313 Golgi
inheritance [PMID:11285273]"
/experiment="EXISTENCE:physical interaction:GO:0000011
vacuole inheritance [PMID:12594460]"
/experiment="EXISTENCE:physical interaction:GO:0000132
establishment of mitotic spindle orientation
[PMID:10984058]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:27307583]"
/experiment="EXISTENCE:physical interaction:GO:0031267
small GTPase binding [PMID:12391144]"
/experiment="EXISTENCE:physical interaction:GO:0045033
peroxisome inheritance [PMID:16678774]"
/experiment="EXISTENCE:physical interaction:GO:0071563
Myo2p-Vac17p-Vac8p transport complex [PMID:12594460]"
/note="Type V myosin motor involved in actin-based
transport of cargos; required for the polarized delivery
of secretory vesicles, the vacuole, late Golgi elements,
peroxisomes, lipid droplets, and the mitotic spindle;
effector of Ypt11p, a rab-type small GTPase; MYO2 has a
paralog, MYO4, that arose from the whole genome
duplication"
/codon_start=1
/product="myosin 2"
/protein_id="NP_014971.1"
/db_xref="GeneID:854504"
/db_xref="SGD:S000005853"
/translation="MSFEVGTRCWYPHKELGWIGAEVIKNEFNDGKYHLELQLEDDEI
VSVDTKDLNNDKDQSLPLLRNPPILEATEDLTSLSYLNEPAVLHAIKQRYSQLNIYTY
SGIVLIATNPFDRVDQLYTQDMIQAYAGKRRGELEPHLFAIAEEAYRLMKNDKQNQTI
VVSGESGAGKTVSAKYIMRYFASVEEENSATVQHQVEMSETEQKILATNPIMEAFGNA
KTTRNDNSSRFGKYLEILFDKDTSIIGARIRTYLLERSRLVYQPPIERNYHIFYQLMA
GLPAQTKEELHLTDASDYFYMNQGGDTKINGIDDAKEYKITVDALTLVGITKETQHQI
FKILAALLHIGNIEIKKTRNDASLSADEPNLKLACELLGIDAYNFAKWVTKKQIITRS
EKIVSNLNYSQALVAKDSVAKFIYSALFDWLVENINTVLCNPAVNDQISSFIGVLDIY
GFEHFEKNSFEQFCINYANEKLQQEFNQHVFKLEQEEYVKEEIEWSFIEFNDNQPCID
LIENKLGILSLLDEESRLPAGSDESWTQKLYQTLDKSPTNKVFSKPRFGQTKFIVSHY
ALDVAYDVEGFIEKNRDTVSDGHLEVLKASTNETLINILEGLEKAAKKLEEAKKLELE
QAGSKKPGPIRTVNRKPTLGSMFKQSLIELMNTINSTNVHYIRCIKPNADKEAWQFDN
LMVLSQLRACGVLETIRISCAGFPSRWTFEEFVLRYYILIPHEQWDLIFKKKETTEED
IISVVKMILDATVKDKSKYQIGNTKIFFKAGMLAYLEKLRSNKMHNSIVMIQKKIRAK
YYRKQYLQISQAIKYLQNNIKGFIIRQRVNDEMKVNCATLLQAAYRGHSIRANVFSVL
RTITNLQKKIRKELKQRQLKQEHEYNAAVTIQSKVRTFEPRSRFLRTKKDTVVVQSLI
RRRAAQRKLKQLKADAKSVNHLKEVSYKLENKVIELTQNLASKVKENKEMTERIKELQ
VQVEESAKLQETLENMKKEHLIDIDNQKSKDMELQKTIENNLQSTEQTLKDAQLELED
MVKQHDELKEESKKQLEELEQTKKTLVEYQTLNGDLQNEVKSLKEEIARLQTAMSLGT
VTTSVLPQTPLKDVMGGGASNFNNMMLENSDLSPNDLNLKSRSTPSSGNNHIDSLSVD
RENGVNATQINEELYRLLEDTEILNQEITEGLLKGFEVPDAGVAIQLSKRDVVYPARI
LIIVLSEMWRFGLTKQSESFLAQVLTTIQKVVTQLKGNDLIPSGVFWLANVRELYSFV
VFALNSILTEETFKNGMTDEEYKEYVSLVTELKDDFEALSYNIYNIWLKKLQKQLQKK
AINAVVISESLPGFSAGETSGFLNKIFANTEEYTMDDILTFFNSIYWCMKSFHIENEV
FHAVVTTLLNYVDAICFNELIMKRNFLSWKRGLQLNYNVTRLEEWCKTHGLTDGTECL
QHLIQTAKLLQVRKYTIEDIDILRGICYSLTPAQLQKLISQYQVADYESPIPQEILRY
VADIVKKEAALSSSGNDSKGHEHSSSIFITPETGPFTDPFSLIKTRKFDQVEAYIPAW
LSLPSTKRIVDLVAQQVVQDGH"
gene complement(<930734..>931081)
/gene="SNC2"
/locus_tag="YOR327C"
/db_xref="GeneID:854505"
mRNA complement(<930734..>931081)
/gene="SNC2"
/locus_tag="YOR327C"
/product="SNAP receptor SNC2"
/transcript_id="NM_001183747.3"
/db_xref="GeneID:854505"
CDS complement(930734..931081)
/gene="SNC2"
/locus_tag="YOR327C"
/experiment="EXISTENCE:direct assay:GO:0005484 SNAP
receptor activity [PMID:14981247]"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:16452633]"
/experiment="EXISTENCE:direct assay:GO:0005802 trans-Golgi
network [PMID:36125415|PMID:16452633]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:36125415]"
/experiment="EXISTENCE:direct assay:GO:0005933 cellular
bud [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0006906 vesicle
fusion [PMID:11739407]"
/experiment="EXISTENCE:direct assay:GO:0030658 transport
vesicle membrane [PMID:15821745]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0006897
endocytosis [PMID:11029060]"
/experiment="EXISTENCE:mutant phenotype:GO:0006887
exocytosis [PMID:9195971]"
/experiment="EXISTENCE:mutant phenotype:GO:0006893 Golgi
to plasma membrane transport [PMID:8374953]"
/experiment="EXISTENCE:physical interaction:GO:0031201
SNARE complex [PMID:9731774]"
/note="Vesicle membrane receptor protein (v-SNARE);
involved in the fusion between Golgi-derived secretory
vesicles with the plasma membrane; Snc2p levels regulated
by Vps45p; member of the synaptobrevin/VAMP family of
R-type v-SNARE proteins; SNC2 has a paralog, SNC1, that
arose from the whole genome duplication"
/codon_start=1
/product="SNAP receptor SNC2"
/protein_id="NP_014972.3"
/db_xref="GeneID:854505"
/db_xref="SGD:S000005854"
/translation="MSSSVPYDPYVPPEESNSGANPNSQNKTAALRQEIDDTVGIMRD
NINKVAERGERLTSIEDKADNLAISAQGFKRGANRVRKQMWWKDLKMRMCLFLVVIIL
LVVIIVPIVVHFS"
gene <931803..>936497
/gene="PDR10"
/locus_tag="YOR328W"
/db_xref="GeneID:854506"
mRNA <931803..>936497
/gene="PDR10"
/locus_tag="YOR328W"
/product="ATP-binding cassette multidrug transporter
PDR10"
/transcript_id="NM_001183748.1"
/db_xref="GeneID:854506"
CDS 931803..936497
/gene="PDR10"
/locus_tag="YOR328W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:19452121]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:1990414
replication-born double-strand break repair via sister
chromatid exchange [PMID:23357952]"
/note="ATP-binding cassette (ABC) transporter; multidrug
transporter involved in the pleiotropic drug resistance
network; regulated by Pdr1p and Pdr3p"
/codon_start=1
/product="ATP-binding cassette multidrug transporter
PDR10"
/protein_id="NP_014973.1"
/db_xref="GeneID:854506"
/db_xref="SGD:S000005855"
/translation="MLQAPSSSNSGLNQGNAAPDGPPNETQPYEGLDAAAQEEIKELA
RTLTSQSSLLSQEKRITGTGDPNTLTAASSSSLSRSIFASDIKGVNPILLDVNDPDYD
ETLDPRSENFSSVRWVRNMAQICENDSDFYKPFSLGCAWKDLSASGDSADITYQGTFG
NMPIKYLKMSWRCISRRLFHRTHGKSEDNDSGFQILKPMDGCINPGELLVVLGRPGAG
CTTLLKSISVNTHGFKISPDTIITYNGFSNKEIKNHYRGEVVYNAESDIHIPHLTVFQ
TLYTVARLKTPRNRIKGVDRDTFAKHMTEVAMATYGLSHTADTKVGNDFVRGVSGGER
KRVSIAEVSICGSKFQCWDNATRGLDSATALEFIKALKTQATITKSAATVAIYQCSKD
AYDLFDKVCVLYDGYQIFFGPSKQAKKYFQRMGYVCPERQTTADYLTSITSPSERIKD
KDMVKHGIMIPQTAYEMNQYWIQSEEYKQLQVQVNKHLDTDSSQQREQIKNAHIAKQS
KRARPSSPYTVSFFLQVKYILIRDIWRIKNDPSIQLFTVLSHAAMALILGSMFYEVML
STTTTTFYYRGAAIFFAILFNAFSSLLEIFSLYETRPITEKHKTYSLYRPSADAFAST
FSDVPTKLATAVTFNIPYYFLINLKRDAGAFFFYFLINIITVFAMSHLFRCIGSVSKT
LPQAMVPASVLLLAFAMYTGFAIPRVQMLGWSKWISYINPLSYLFESLMINEFHGRNF
PCAQYIPSGPNYVNATGDEVTCSALGSIPGNNYVSGDDFIQTNYGYRHKNKWRSVGIG
LAYIIFFLFLYLFFCEYNEGAKQNGEMLVFPHSVVKKMKKKGIVSEKKKKNQPTLSTS
DAEKDVEMNNNSSATDSRFLRDSDAAIMGNDKTVAKEHYSSPSSSASQSNSFSKSDDI
ELSKSQAIFHWKNLCYDIPIKNGKRRILDNVDGWVKPGTLTALIGASGAGKTTLLDCL
AERTTMGLITGDVFVDGRPRDQSFPRSIGYCQQQDLHLKTATVRESLRFSAYLRQADD
VSIEEKDKYVEEVIEVLEMKLYADAIVGVPGEGLNVEQRKRLTIGVELAAKPKLLVFL
DEPTSGLDSQTAWSTCQLMKKLASRGQAILCTIHQPSALLMQEFDRLLFLQEGGQTVY
FGELGKGCKTMINYFEAHGAHKCPPDANPAEWMLEIVGAAPGTHASQDYFAIWRDSEE
YREMQKELDWMERELPKRTEGSSNEEQKEFATSTLYQIKLVSYRLFHQYWRTPFYLWS
KFFSTIVSELFIGFTFFKANTSLQGLQNQMLAIFMFTVVFNPILQQYLPLFVQQRELY
EARERPSRTFSWKAFIVSQILVEIPWNLLAGTIAFFVYYYPVGFYRNASYANQLHERG
ALFWLFACAFYVYISSMGVLVISCIEIAENAANLASLFFIMSLSFCGVLATPNILPRF
WIFMYRVSPLTYLIDALLSVGLANASVVCSSNELLKIVPPSGMTCSEYMEPYMQSTGT
GYLLDGSSETECHFCQFSSTNDYLATVSSSYSRRWMNYGIFSAYIVFDYCAAIFLYWL
VRVPKKSKKLKK"
gene complement(<936731..>939349)
/gene="SCD5"
/locus_tag="YOR329C"
/gene_synonym="FTB1; SCD7"
/db_xref="GeneID:854507"
mRNA complement(<936731..>939349)
/gene="SCD5"
/locus_tag="YOR329C"
/gene_synonym="FTB1; SCD7"
/product="Scd5p"
/transcript_id="NM_001183749.3"
/db_xref="GeneID:854507"
CDS complement(936731..939349)
/gene="SCD5"
/locus_tag="YOR329C"
/gene_synonym="FTB1; SCD7"
/experiment="EXISTENCE:direct assay:GO:0008157 protein
phosphatase 1 binding [PMID:12356757]"
/experiment="EXISTENCE:direct assay:GO:0030479 actin
cortical patch [PMID:12181333]"
/experiment="EXISTENCE:genetic interaction:GO:0030866
cortical actin cytoskeleton organization [PMID:12181333]"
/experiment="EXISTENCE:genetic interaction:GO:2000370
positive regulation of clathrin-dependent endocytosis
[PMID:12356757]"
/experiment="EXISTENCE:mutant phenotype:GO:0005634 nucleus
[PMID:16251346]"
/experiment="EXISTENCE:mutant phenotype:GO:0009306 protein
secretion [PMID:8688556]"
/experiment="EXISTENCE:mutant phenotype:GO:0030866
cortical actin cytoskeleton organization [PMID:12956961]"
/experiment="EXISTENCE:mutant phenotype:GO:2000370
positive regulation of clathrin-dependent endocytosis
[PMID:12356757]"
/experiment="EXISTENCE:physical interaction:GO:0008157
protein phosphatase 1 binding [PMID:8754819]"
/note="Protein required for normal actin organization and
endocytosis; targeting subunit for protein phosphatase
type 1; undergoes Crm1p-dependent nuclear-cytoplasmic
shuttling; multicopy suppressor of clathrin deficiency"
/codon_start=1
/product="Scd5p"
/protein_id="NP_014974.3"
/db_xref="GeneID:854507"
/db_xref="SGD:S000005856"
/translation="MSFDWLNVPGLDLSSGDQAEKRPSNGLGPPSVSFDFGINTAAPH
DSSFWDQGSRSHSDTTLSYRNNHSNTAADNATNVSSPQKDNPPNGEVRTLSGGDVYAE
SPEDMQVPLSLSQNQLTHEEIRTYLRWYHYICLRTHGKLVRLNDVFRFLTNFNLSQKV
KDRIVEIFRSCKNALNIGQFFAVLRLVSRAIIYGILPLRRMILEKAPVPKPRPILSSE
NHEEVYEEVEDDDSSAKTGDQKVDFDSFASLLLTGKTTRKRVRRRIKNLNFKSKKVRF
SEHITFQDPPNLNQESSNNSEARKQDPDAEDEDQDSNNDSPLDFTLPMDQLLKRLYKR
RKNSGLVSSLPSEQQETEEEKKVLEDMKDSLSHFKQIQTVDSASLPISSVFLQNGNTL
PTSNVNNTTVPQQLPLEPLKPTATGSANHLVREEYNQGLHPSNGAIQTGLQPLKPTAT
GSANYLMRSHMEQPQSIKPSSTPETVTNSGGLQPLKPTATGSANYLMKQHISPSVNNP
VSSMFQAQFTNQSSSPQSTGPAFLNSPNITLPQSNQQQPYQEVNPTQAKIEPSNISPQ
HTYSNNVRINNGNIVSMPKVEITGAFPPQNTLPQHQQSHLLSPQNTIPQHQRSQLISP
QNTFTQNQPILSPQHTYSNNQATMISPQNTYTNNQQQPQHLPPPPPPRAQQQQQGAIV
PPQHMYSNVQKQNNLVPTQPSYTNSPSIQSPNFLSPQNAANSYFQSLLSSSPSPNPTP
SNASTVNGNNASNGISSFQNTSAAMNNTQSHQTYIQQQQQQQTQQRIYGGQLSQMQQH
PGQLHLNNSDIHSQPNKPNYGMLGQQVHQQQQQQQQQFPFTADVNRSNSSDILGNLQS
LQQQVDALQIQYNRRP"
gene complement(<939621..>943385)
/gene="MIP1"
/locus_tag="YOR330C"
/db_xref="GeneID:854508"
mRNA complement(<939621..>943385)
/gene="MIP1"
/locus_tag="YOR330C"
/product="DNA-directed DNA polymerase gamma MIP1"
/transcript_id="NM_001183750.1"
/db_xref="GeneID:854508"
CDS complement(939621..943385)
/gene="MIP1"
/locus_tag="YOR330C"
/EC_number="2.7.7.7"
/experiment="EXISTENCE:direct assay:GO:0003887
DNA-directed DNA polymerase activity [PMID:20601675]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:10567545|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0006264
mitochondrial DNA replication [PMID:12023279]"
/experiment="EXISTENCE:direct assay:GO:0008408 3'-5'
exonuclease activity [PMID:20601675]"
/experiment="EXISTENCE:mutant phenotype:GO:0006264
mitochondrial DNA replication [PMID:2684980]"
/experiment="EXISTENCE:mutant phenotype:GO:0006995
cellular response to nitrogen starvation [PMID:29519802]"
/experiment="EXISTENCE:mutant phenotype:GO:0032043
mitochondrial DNA catabolic process [PMID:29519802]"
/note="Mitochondrial DNA polymerase gamma; single subunit
of mitochondrial DNA polymerase in yeast, in contrast to
metazoan complex of catalytic and accessory subunits;
polymorphic in yeast, petites occur more frequently in
some lab strains; human ortholog POLG complements yeast
mip1 mutant; mutations in human POLG associated with
Alpers-Huttenlocher syndrome (AHS), progressive external
ophthalmoplegia (PEO), parkinsonism, other mitochondrial
diseases"
/codon_start=1
/product="DNA-directed DNA polymerase gamma MIP1"
/protein_id="NP_014975.2"
/db_xref="GeneID:854508"
/db_xref="SGD:S000005857"
/translation="MTKLMVRSECMLRMVRRRPLRVQFCARWFSTKKNTAEAPRINPV
GIQYLGESLQRQVFGSCGGKDEVEQSDKLMELSKKSLKDHGLWGKKTLITDPISFPLP
PLQGRSLDEHFQKIGRFNSEPYKSFCEDKFTEMVARPAEWLRKPGWVKYVPGMAPVEV
AYPDEELVVFDVETLYNVSDYPTLATALSSTAWYLWCSPFICGGDDPAALIPLNTLNK
EQVIIGHNVAYDRARVLEEYNFRDSKAFFLDTQSLHIASFGLCSRQRPMFMKNNKKKE
AEVESEVHPEISIEDYDDPWLNVSALNSLKDVAKFHCKIDLDKTDRDFFASTDKSTII
ENFQKLVNYCATDVTATSQVFDEIFPVFLKKCPHPVSFAGLKSLSKCILPTKLNDWND
YLNSSESLYQQSKVQIESKIVQIIKDIVLLKDKPDFYLKDPWLSQLDWTTKPLRLTKK
GVPAKCQKLPGFPEWYRQLFPSKDTVEPKITIKSRIIPILFKLSWENSPVIWSKESGW
CFNVPHEQVETYKAKNYVLADSVSQEEEEIRTHNLGLQCTGVLFKVPHPNGPTFNCTN
LLTKSYNHFFEKGVLKSESELAHQALQINSSGSYWMSARERIQSQFVVPSCKFPNEFQ
SLSAKSSLNNEKTNDLAIIIPKIVPMGTITRRAVENAWLTASNAKANRIGSELKTQVK
APPGYCFVGADVDSEELWIASLVGDSIFNVHGGTAIGWMCLEGTKNEGTDLHTKTAQI
LGCSRNEAKIFNYGRIYGAGAKFASQLLKRFNPSLTDEETKKIANKLYENTKGKTKRS
KLFKKFWYGGSESILFNKLESIAEQETPKTPVLGCGITYSLMKKNLRANSFLPSRINW
AIQSSGVDYLHLLCCSMEYIIKKYNLEARLCISIHDEIRFLVSEKDKYRAAMALQISN
IWTRAMFCQQMGINELPQNCAFFSQVDIDSVIRKEVNMDCITPSNKTAIPHGEALDIN
QLLDKSNSKLGKPNLDIDSKVSQYAYNYREPVFEEYNKSYTPEFLKYFLAMQVQSDKR
DVNRLEDEYLRECTSKEYARDGNTAEYSLLDYIKDVEKGKRTKVRIMGSNFLDGTKNA
KADQRIRLPVNMPDYPTLHKIANDSAIPEKQLLENRRKKENRIDDENKKKLTRKKNTT
PMERKYKRVYGGRKAFEAFYECANKPLDYTLETEKQFFNIPIDGVIDDVLNDKSNYKK
KPSQARTASSSPIRKTAKAVHSKKLPARKSSTTNRNLVELERDITISREY"
gene <943656..>944357
/gene="VMA4"
/locus_tag="YOR332W"
/db_xref="GeneID:854509"
mRNA <943656..>944357
/gene="VMA4"
/locus_tag="YOR332W"
/product="H(+)-transporting V1 sector ATPase subunit E"
/transcript_id="NM_001183752.3"
/db_xref="GeneID:854509"
CDS 943656..944357
/gene="VMA4"
/locus_tag="YOR332W"
/experiment="EXISTENCE:direct assay:GO:0000221 vacuolar
proton-transporting V-type ATPase, V1 domain
[PMID:16774922]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane
[PMID:23836928|PMID:26928762|PMID:23708375]"
/experiment="EXISTENCE:direct assay:GO:0045121 membrane
raft [PMID:23836928]"
/note="Subunit E of the V1 domain of the vacuolar
H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton
pump found throughout the endomembrane system; V1 domain
has eight subunits; required for the V1 domain to assemble
onto the vacuolar membrane; protein abundance increases in
response to DNA replication stress"
/codon_start=1
/product="H(+)-transporting V1 sector ATPase subunit E"
/protein_id="NP_014977.3"
/db_xref="GeneID:854509"
/db_xref="SGD:S000005859"
/translation="MSSAITALTPNQVNDELNKMQAFIRKEAEEKAKEIQLKADQEYE
IEKTNIVRNETNNIDGNFKSKLKKAMLSQQITKSTIANKMRLKVLSAREQSLDGIFEE
TKEKLSGIANNRDEYKPILQSLIVEALLKLLEPKAIVKALERDVDLIESMKDDIMREY
GEKAQRAPLEEIVISNDYLNKDLVSGGVVVSNASDKIEINNTLEERLKLLSEEALPAI
RLELYGPSKTRKFFD"
gene <944596..>946008
/gene="MRS2"
/locus_tag="YOR334W"
/db_xref="GeneID:854511"
mRNA <944596..>946008
/gene="MRS2"
/locus_tag="YOR334W"
/product="Mrs2p"
/transcript_id="NM_001183754.1"
/db_xref="GeneID:854511"
CDS 944596..946008
/gene="MRS2"
/locus_tag="YOR334W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:10400670]"
/experiment="EXISTENCE:direct assay:GO:0015095 magnesium
ion transmembrane transporter activity [PMID:12628916]"
/experiment="EXISTENCE:direct assay:GO:0045016
mitochondrial magnesium ion transmembrane transport
[PMID:12628916]"
/experiment="EXISTENCE:direct assay:GO:1901612 cardiolipin
binding [PMID:37721533]"
/experiment="EXISTENCE:mutant phenotype:GO:0015095
magnesium ion transmembrane transporter activity
[PMID:10400670]"
/experiment="EXISTENCE:mutant phenotype:GO:0045016
mitochondrial magnesium ion transmembrane transport
[PMID:10400670]"
/note="Mitochondrial inner membrane Mg(2+) channel;
required for maintenance of intramitochondrial Mg(2+)
concentrations at the correct level to support splicing of
group II introns; similar to bacterial CorA"
/codon_start=1
/product="Mrs2p"
/protein_id="NP_014979.1"
/db_xref="GeneID:854511"
/db_xref="SGD:S000005861"
/translation="MNRRLLVRSISCFQPLSRITFGRPNTPFLRKYADTSTAANTNST
ILRKQLLSLKPISASDSLFISCTVFNSKGNIISMSEKFPKWSFLTEHSLFPRDLRKID
NSSIDIIPTIMCKPNCIVINLLHIKALIERDKVYVFDTTNPSAAAKLSVLMYDLESKL
SSTKNNSQFYEHRALESIFINVMSALETDFKLHSQICIQILNDLENEVNRLKLRHLLI
KSKDLTLFYQKTLLIRDLLDELLENDDDLANMYLTVKKSPKDNFSDLEMLIETYYTQC
DEYVQQSESLIQDIKSTEEIVNIILDANRNSLMLLELKVTIYTLGFTVASVLPAFYGM
NLKNFIEESEWGFTSVAVFSIVSALYITKKNFNSLRSVTKMTMYPNSPANSSVYPKTS
ASIALTNKLKRRRKWWKSTKQRLGVLLYGSSYTNKANLSNNKINKGFSKVKKFNMEND
IKNKQNRDMIWKWLIEDKKN"
gene complement(<946233..>949109)
/gene="ALA1"
/locus_tag="YOR335C"
/gene_synonym="CDC64"
/db_xref="GeneID:854513"
mRNA complement(<946233..>949109)
/gene="ALA1"
/locus_tag="YOR335C"
/gene_synonym="CDC64"
/product="alanine--tRNA ligase"
/transcript_id="NM_001183755.3"
/db_xref="GeneID:854513"
CDS complement(946233..949109)
/gene="ALA1"
/locus_tag="YOR335C"
/gene_synonym="CDC64"
/EC_number="6.1.1.7"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276|PMID:16556230|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:16556230|PMID:16823961|PMID:14576278|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:1990825
sequence-specific mRNA binding [PMID:34039240]"
/experiment="EXISTENCE:mutant phenotype:GO:0004813
alanine-tRNA ligase activity [PMID:7761427]"
/experiment="EXISTENCE:mutant phenotype:GO:0006419
alanyl-tRNA aminoacylation [PMID:7761427]"
/note="Cytoplasmic and mitochondrial alanyl-tRNA
synthetase; required for protein synthesis; point mutation
(cdc64-1 allele) causes cell cycle arrest at G1; lethality
of null mutation is functionally complemented by human
homolog AARS; mutations in human homolog AARS are
associated with autoimmune disease
polymyositis/dermatomyositis and with hereditary
peripheral neuropathy, Charcot-Marie-Tooth (CMT) disease"
/codon_start=1
/product="alanine--tRNA ligase"
/protein_id="NP_014980.3"
/db_xref="GeneID:854513"
/db_xref="SGD:S000005862"
/translation="MTIGDKQKWTATNVRNTFLDYFKSKEHKFVKSSPVVPFDDPTLL
FANAGMNQYKPIFLGTVDPASDFYTLKRAYNSQKCIRAGGKHNDLEDVGKDSYHHTFF
EMLGNWSFGDYFKKEAITYSWTLLTEVYGIPKDRLYVTYFEGDEKLGLEPDTEARELW
KNVGVPDDHILPGNAKDNFWEMGDQGPCGPCSEIHYDRIGGRNAASLVNMDDPDVLEV
WNLVFIQFNREQDGSLKPLPAKHIDTGMGFERLVSVLQDVRSNYDTDVFTPLFERIQE
ITSVRPYSGNFGENDKDGIDTAYRVLADHVRTLTFALADGGVPNNEGRGYVLRRILRR
GARYARKYMNYPIGNFFSTLAPTLISQVQDIFPELAKDPAFLFEILDEEEASFAKTLD
RGERLFEKYASAASKTESKTLDGKQVWRLYDTYGFPVDLTELMAEEQGLKIDGPGFEK
AKQESYEASKRGGKKDQSDLIKLNVHELSELNDAKVPKTNDEFKYGSANVEGTILKLH
DGTNFVDEITEPGKKYGIILDKTCFYAEQGGQEYDTGKIVIDDAAEFNVENVQLYNGF
VFHTGSLEEGKLSVGDKIIASFDELRRFPIKNNHTGTHILNFALKETLGNDVDQKGSL
VAPEKLRFDFSHKKAVSNEELKKVEDICNEQIKENLQVFYKEIPLDLAKSIDGVRAVF
GETYPDPVRVVSVGKPIEELLANPANEEWTKYSIEFCGGTHVNKTGDIKYFVILEESG
IAKGIRRIVAVTGTEAFEAQRLAEQFAADLDAADKLPFSPIKEKKLKELGVKLGQLSI
SVITKNELKQKFNKIEKAVKDEVKSRAKKENKQTLDEVKTFFETNENAPYLVKFIDIS
PNAKAITEAINYMKSNDSVKDKSIYLLAGNDPEGRVAHGCYISNAALAKGIDGSALAK
KVSSIIGGKAGGKGNVFQGMGDKPAAIKDAVDDLESLFKEKLSI"
gene <949773..>953870
/gene="KRE5"
/locus_tag="YOR336W"
/db_xref="GeneID:854514"
mRNA <949773..>953870
/gene="KRE5"
/locus_tag="YOR336W"
/product="Kre5p"
/transcript_id="NM_001183756.1"
/db_xref="GeneID:854514"
CDS 949773..953870
/gene="KRE5"
/locus_tag="YOR336W"
/experiment="EXISTENCE:direct assay:GO:0003980
UDP-glucose:glycoprotein glucosyltransferase activity
[PMID:9611196]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0070880
fungal-type cell wall beta-glucan biosynthetic process
[PMID:9430631]"
/note="Protein required for beta-1,6 glucan biosynthesis;
mutations result in aberrant morphology and severe growth
defects"
/codon_start=1
/product="Kre5p"
/protein_id="NP_014981.1"
/db_xref="GeneID:854514"
/db_xref="SGD:S000005863"
/translation="MRLLALVLLLLCAPLRAWTYSLRYGIPESAQVWSILVHLLGDVD
NQLLTNLYPLVTGLDDEIDIQENLVALTSNVLRERYDKEDVADLLELYASLYPMGMIQ
HDISSNAEQDDANSSYFVLNGNRYEKPDDVFYLKSKDLTIQQKVPDVDVIQPYDVVIG
TNSEAPILILYGCPTVIDSDFEEFNRNLFMEAMNGEGKFRFIWRSTCSLDGKSVEYPL
THPLEITLQNGSRMSSIPQLKKILYTVPKEILVGADNDDQLHDLEPEELRELDLRVTS
LISEFYQYKKDITATLNFTKSIVNNFPLISKQLIKVSSVNKDIITSNEELNSKGFDYN
MLGLYINGQNWKITSLTPYNLLTALKTEYQSLLKITNLLQELEPSKCILDSKFLLNKF
SQFSLGKLQNLQPIKMDLHTIPGFSESVIYFNDIESDPQYDELVNSVQAFFDKSKFGE
LPEIKQNWSEIIFVIDFARLEDSEVKEALGGLVRAVNVVSQGYPQRVGLLPFSSDSDK
SVVNKIYELKNSTDNLTELKSFLETMLLADGLSANAKHSKHIPVPDVFHLLDELQIDE
TSIIINGEIYPFRKNAWNYLIAKVIKKDTEFIRKELSNSSPKNKQISVRDLLHYKSAN
LRHNKYTPNYFADSVYSSVNNTALESVCSERIGYYTKNEEYNLLHTITLVDDFGSIHA
LKRLRNLLHTSFVGVRIRIIHVGDISDIWYQLRGSLSQKDPIGSINTFIDALKLKKVK
SHTYKKSGLNQLGLHKWLPDIPLFELQKGSFIALNGRFIHLDQNEVPETEHFEAIIKR
EALRTIDSVFALDLLFPGFSQEIINPDLIEMISSILTRLFYQGTHIYNNGIDYTTESS
LPRMDLSEFFRPNNLTMFEDGKSASIDLLLILDPLEERTQMILSLVEQFRPLKFVNIQ
VILMPTLELNIVPIRRIYVDDADIVKSITSEDSRSDPEVDIEMDVPNSFIVDNNYRIK
KLLIELHSFSSKTVLSTGNIDGMGGVCLALVDSAGNIIDKTTTMKTFGYGQFHTDKFL
KGCYIKSCDSRYTVQSFSTDGHPDFIPSDSLDILSYNPQKIAVKISEEPTHEEEYEEG
RNNDTIINIFTILESGPDEEERYMQMILSILSKCPETQKVNFFILDQPFISDTLRKSC
EYINSSDEMRGNVIFLNYEWPQWLRPQRFSSRRRDVSRFLFLDVLLPQNISKVLYMSP
TEVPLDPFDIFQFQGLKRAPLGLFRMSGDGYWKEGYWEKMLRENNLEFYSTEPAFLVN
LERFRELDAGDKYRIHYQRISTDAMSLVNIGQDLVNNLQLEVPIRFLKGSYKKKLVIN
DECVSEWKKKINKFASSPGDEDVPGESVSSKYQDSDNAAPLHDEL"
gene <954344..>956623
/gene="TEA1"
/locus_tag="YOR337W"
/db_xref="GeneID:854515"
mRNA <954344..>956623
/gene="TEA1"
/locus_tag="YOR337W"
/product="Tea1p"
/transcript_id="NM_001183757.3"
/db_xref="GeneID:854515"
CDS 954344..956623
/gene="TEA1"
/locus_tag="YOR337W"
/experiment="EXISTENCE:direct assay:GO:0003677 DNA binding
[PMID:8524314]"
/experiment="EXISTENCE:direct assay:GO:0006368
transcription elongation by RNA polymerase II
[PMID:8524314]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:19111667]"
/experiment="EXISTENCE:direct assay:GO:0051285 cell cortex
of cell tip [PMID:23673619]"
/note="Ty1 enhancer activator involved in Ty
enhancer-mediated transcription; required for full levels
of Ty enhancer-mediated transcription; C6 zinc cluster
DNA-binding protein"
/codon_start=1
/product="Tea1p"
/protein_id="NP_014982.3"
/db_xref="GeneID:854515"
/db_xref="SGD:S000005864"
/translation="MTAPLWPNKNEKNHTVKRALSTDMTSNILSSTNASSNEENSRSS
SAANVRSGTGANTLTNGGSTRKRLACTNCRNRRKKCDLGFPCGNCSRLELVCNVNDED
LRKKRYTNKYVKSLESHIAQLETNLKNLVQKIYPDDEQILNRMMVGDVLSALPDSSQV
SINYTDQTPSLPIPATRGTFIIENDKVSQPLSSFNQQTEPSTLNSGIFNTQKQNFEES
LDDQLLLRRSLTPQGEKKKKPLVKGSLYPEGPVSYKRKHPVKSDSLLPVSSLTAATDP
STFSDGITAGNSVLVNGELKKRISDLKTTVIVRGLNDDNPNSINNDPRILKSLSNFYK
WLYPGYFIFVHRESFLYGFFNHSKNNYEDSSYCSVELIYAMCAVGSRLTPDLQEYSEV
YYQRSKKTLLQLVFDEQSTARITTVQALFCLAFYELGKGNNQLGWYFSGLAIRVGYDM
GFQLDPKVWYVDDNNLQLTQSELEIRSRIYWGCYIADHFICLMLGRTSTLSVSNSTMP
ESDELPEVNGTEEFRFIGRHVLQISLPLKNLIILSRLVQIFTSKIFIESEDIARKLKY
LNTFNSQVYNWRQSLPEFLQWSKTLIENDDVSTDPTISYFWYCYYIVRLTFNKPFIED
SQESETVVIEIIDDLKTLLDNFGKKFGNYTKGNLYQLYSCLLAINCLKKLKEIRSSEQ
DSWNAQLDFFNHIFYTQLYPAYDLPKKLQEDTELETEQENQMLNQVGNINYTHDFSLS
HEIDDLIRELFGVGTPQKL"
gene <956898..>957989
/locus_tag="YOR338W"
/db_xref="GeneID:854516"
mRNA <956898..>957989
/locus_tag="YOR338W"
/product="uncharacterized protein"
/transcript_id="NM_001183758.1"
/db_xref="GeneID:854516"
CDS 956898..957989
/locus_tag="YOR338W"
/experiment="EXISTENCE:mutant phenotype:GO:0030437
ascospore formation [PMID:12586695]"
/note="hypothetical protein; YOR338W transcription is
regulated by Azf1p and its transcript is a specific target
of the G protein effector Scp160p; identified as being
required for sporulation in a high-throughput mutant
screen; YOR338W has a paralog, FUN19, that arose from the
whole genome duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014983.1"
/db_xref="GeneID:854516"
/db_xref="SGD:S000005865"
/translation="MLDNMQFHSPAPEHPQLNGGINKIPASHKIGYKLNQQVQRLAVV
RNNIEERLNSMESSHGQISDSSVVRAIDASIDDFLIPSPPLSPKLRQCPIISQPQLVN
VESDHRELIMLTPVWEAGLNSQKYNHNTRNFLSQYSFFRDMKTTKRIPNKENRKLKVV
KSVVNSEALPKRRRYDRKIKRRSRELYEDDGNRSENYDEESAQEVPVRSVTPIRQVKR
SLHTISSPLASQGVVNNVPKYIPSMSWEKLPDYSPPLHTLPNSNNKVLKVEWKGSPMD
LNHDPLKQRLHPAELVLAQILRLPCDLYLDSKRRFFLEKVHRFKKGLPFRRTDAQKAC
RIDVNKASRLFAAFEKVGWLQDKHFEKYL"
gene complement(<958362..>958832)
/gene="UBC11"
/locus_tag="YOR339C"
/db_xref="GeneID:854517"
mRNA complement(<958362..>958832)
/gene="UBC11"
/locus_tag="YOR339C"
/product="putative E2 ubiquitin-protein ligase UBC11"
/transcript_id="NM_001183759.3"
/db_xref="GeneID:854517"
CDS complement(958362..958832)
/gene="UBC11"
/locus_tag="YOR339C"
/EC_number="2.3.2.23"
/experiment="EXISTENCE:curator inference:GO:0005737
cytoplasm [PMID:8982460]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:29519818]"
/note="Ubiquitin-conjugating enzyme; most similar in
sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but
not a true functional homolog of this E2; unlike E2-C, not
required for the degradation of mitotic cyclin Clb2"
/codon_start=1
/product="putative E2 ubiquitin-protein ligase UBC11"
/protein_id="NP_014984.3"
/db_xref="GeneID:854517"
/db_xref="SGD:S000005866"
/translation="MAVEEGGCVTKRLQNELLQLLSSTTESISAFPVDDNDLTYWVGY
ITGPKDTPYSGLKFKVSLKFPQNYPFHPPMIKFLSPMWHPNVDKSGNICLDILKEKWS
AVYNVETILLSLQSLLGEPNNRSPLNAVAAELWDADMEEYRKKVLACYEEIDDY"
gene complement(<959202..>960182)
/gene="RPA43"
/locus_tag="YOR340C"
/db_xref="GeneID:854518"
mRNA complement(<959202..>960182)
/gene="RPA43"
/locus_tag="YOR340C"
/product="DNA-directed RNA polymerase I subunit RPA43"
/transcript_id="NM_001183760.1"
/db_xref="GeneID:854518"
CDS complement(959202..960182)
/gene="RPA43"
/locus_tag="YOR340C"
/experiment="EXISTENCE:direct assay:GO:0003899
DNA-directed RNA polymerase activity [PMID:9837969]"
/experiment="EXISTENCE:direct assay:GO:0005736 RNA
polymerase I complex [PMID:11486042|PMID:9837969]"
/experiment="EXISTENCE:direct assay:GO:0006360
transcription by RNA polymerase I [PMID:9837969]"
/experiment="EXISTENCE:genetic interaction:GO:0042790
nucleolar large rRNA transcription by RNA polymerase I
[PMID:7592632]"
/note="RNA polymerase I subunit A43"
/codon_start=1
/product="DNA-directed RNA polymerase I subunit RPA43"
/protein_id="NP_014985.1"
/db_xref="GeneID:854518"
/db_xref="SGD:S000005867"
/translation="MSQVKRANENRETARFIKKHKKQVTNPIDEKNGTSNCIVRVPIA
LYVSLAPMYLENPLQGVMKQHLNPLVMKYNNKVGGVVLGYEGLKILDADPLSKEDTSE
KLIKITPDTPFGFTWCHVNLYVWQPQVGDVLEGYIFIQSASHIGLLIHDAFNASIKKN
NIPVDWTFVHNDVEEDADVINTDENNGNNNNEDNKDSNGGSNSLGKFSFGNRSLGHWV
DSNGEPIDGKLRFTVRNVHTTGRVVSVDGTLISDADEEGNGYNSSRSQAESLPIVSNK
KIVFDDEVSIENKESHKELDLPEVKEDNGSEIVYEENTSESNDGESSDSD"
gene <960987..>965981
/gene="RPA190"
/locus_tag="YOR341W"
/gene_synonym="RRN1"
/db_xref="GeneID:854519"
mRNA <960987..>965981
/gene="RPA190"
/locus_tag="YOR341W"
/gene_synonym="RRN1"
/product="DNA-directed RNA polymerase I core subunit
RPA190"
/transcript_id="NM_001183761.3"
/db_xref="GeneID:854519"
CDS 960987..965981
/gene="RPA190"
/locus_tag="YOR341W"
/gene_synonym="RRN1"
/EC_number="2.7.7.6"
/experiment="EXISTENCE:direct assay:GO:0003899
DNA-directed RNA polymerase activity
[PMID:9837969|PMID:3054507]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:15520468]"
/experiment="EXISTENCE:direct assay:GO:0005736 RNA
polymerase I complex [PMID:11486042|PMID:9837969]"
/experiment="EXISTENCE:direct assay:GO:0006360
transcription by RNA polymerase I [PMID:9837969]"
/experiment="EXISTENCE:direct assay:GO:1990841
promoter-specific chromatin binding [PMID:27184763]"
/experiment="EXISTENCE:genetic interaction:GO:0042790
nucleolar large rRNA transcription by RNA polymerase I
[PMID:2023944]"
/experiment="EXISTENCE:mutant phenotype:GO:0042790
nucleolar large rRNA transcription by RNA polymerase I
[PMID:3054507]"
/note="RNA polymerase I largest subunit A190"
/codon_start=1
/product="DNA-directed RNA polymerase I core subunit
RPA190"
/protein_id="NP_014986.3"
/db_xref="GeneID:854519"
/db_xref="SGD:S000005868"
/translation="MDISKPVGSEITSVDFGILTAKEIRNLSAKQITNPTVLDNLGHP
VSGGLYDLALGAFLRNLCSTCGLDEKFCPGHQGHIELPVPCYNPLFFNQLYIYLRASC
LFCHHFRLKSVEVHRYACKLRLLQYGLIDESYKLDEITLGSLNSSMYTDDEAIEDNED
EMDGEGSKQSKDISSTLLNELKSKRSEYVDMAIAKALSDGRTTERGSFTATVNDERKK
LVHEFHKKLLSRGKCDNCGMFSPKFRKDGFTKIFETALNEKQITNNRVKGFIRQDMIK
KQKQAKKLDGSNEASANDEESFDVGRNPTTRPKTGSTYILSTEVKNILDTVFRKEQCV
LQYVFHSRPNLSRKLVKADSFFMDVLVVPPTRFRLPSKLGEEVHENSQNQLLSKVLTT
SLLIRDLNDDLSKLQKDKVSLEDRRVIFSRLMNAFVTIQNDVNAFIDSTKAQGRTSGK
VPIPGVKQALEKKEGLFRKHMMGKRVNYAARSVISPDPNIETNEIGVPPVFAVKLTYP
EPVTAYNIAELRQAVINGPDKWPGATQIQNEDGSLVSLIGMSVEQRKALANQLLTPSS
NVSTHTLNKKVYRHIKNRDVVLMNRQPTLHKASMMGHKVRVLPNEKTLRLHYANTGAY
NADFDGDEMNMHFPQNENARAEALNLANTDSQYLTPTSGSPVRGLIQDHISAGVWLTS
KDSFFTREQYQQYIYGCIRPEDGHTTRSKIVTLPPTIFKPYPLWTGKQIITTVLLNVT
PPDMPGINLISKNKIKNEYWGKGSLENEVLFKDGALLCGILDKSQYGASKYGIVHSLH
EVYGPEVAAKVLSVLGRLFTNYITATAFTCGMDDLRLTAEGNKWRTDILKTSVDTGRE
AAAEVTNLDKDTPADDPELLKRLQEILRDNNKSGILDAVTSSKVNAITSQVVSKCVPD
GTMKKFPCNSMQAMALSGAKGSNVNVSQIMCLLGQQALEGRRVPVMVSGKTLPSFKPY
ETDAMAGGYVKGRFYSGIKPQEYYFHCMAGREGLIDTAVKTSRSGYLQRCLTKQLEGV
HVSYDNSIRDADGTLVQFMYGGDAIDITKESHMTQFEFCLDNYYALLKKYNPSALIEH
LDVESALKYSKKTLKYRKKHSKEPHYKQSVKYDPVLAKYNPAKYLGSVSENFQDKLES
FLDKNSKLFKSSDGVNEKKFRALMQLKYMRSLINPGEAVGIIASQSVGEPSTQMTLNT
FHFAGHGAANVTLGIPRLREIVMTASAAIKTPQMTLPIWNDVSDEQADTFCKSISKVL
LSEVIDKVIVTETTGTSNTAGGNAARSYVIHMRFFDNNEYSEEYDVSKEELQNVISNQ
FIHLLEAAIVKEIKKQKRTTGPDIGVAVPRLQTDVANSSSNSKRLEEDNDEEQSHKKT
KQAVSYDEPDEDEIETMREAEKSSDEEGIDSDKESDSDSEDEDVDMNEQINKSIVEAN
NNMNKVQRDRQSAIISHHRFITKYNFDDESGKWCEFKLELAADTEKLLMVNIVEEICR
KSIIRQIPHIDRCVHPEPENGKRVLVTEGVNFQAMWDQEAFIDVDGITSNDVAAVLKT
YGVEAARNTIVNEINNVFSRYAISVSFRHLDLIADMMTRQGTYLAFNRQGMETSTSSF
MKMSYETTCQFLTKAVLDNEREQLDSPSARIVVGKLNNVGTGSFDVLAKVPNAA"
gene complement(<966666..>967625)
/locus_tag="YOR342C"
/db_xref="GeneID:854520"
mRNA complement(<966666..>967625)
/locus_tag="YOR342C"
/product="uncharacterized protein"
/transcript_id="NM_001183762.1"
/db_xref="GeneID:854520"
CDS complement(966666..967625)
/locus_tag="YOR342C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922|PMID:14562095]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to the cytoplasm and the
nucleus; relocalizes from nucleus to cytoplasm upon DNA
replication stress; YOR342C has a paralog, YAL037W, that
arose from the whole genome duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014987.1"
/db_xref="GeneID:854520"
/db_xref="SGD:S000005869"
/translation="MTILEELNDSSIPQRLDNHIFFGSVHSLTHTDFLVENNIRFFIN
VDLSTELISHIYHEVRSKFAHEIVIVNIDNDSQIPIESDLVRSFHWHNTSLLQQLIHH
LDFLSGINNHGEPLTPPPESHYRNAYVQFDHPSDSVSILDKLLYGNKSEYSRTNIFQV
TNEAKFQVFNDLITIFKYSIAQGGNTNSNILVLSENGSTDENLISLLMSTVLKENPTF
NVYQALQFVKSIAVIPDTVRDEKILWVTGFINYQELIKKNEMYWGLGSQKGRKLTSFA
SPISKVERKQRRRDDQNIMRSKLPQQRQNPFCSTERPKRARCD"
gene complement(<968148..>968474)
/locus_tag="YOR343C"
/db_xref="GeneID:854521"
mRNA complement(<968148..>968474)
/locus_tag="YOR343C"
/product="uncharacterized protein"
/transcript_id="NM_001348881.1"
/db_xref="GeneID:854521"
CDS complement(968148..968474)
/locus_tag="YOR343C"
/note="hypothetical protein; conserved across S.
cerevisiae strains"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335819.1"
/db_xref="GeneID:854521"
/db_xref="SGD:S000005870"
/translation="MEHTAHIFPIIIKGSPPVMSSNPRRQYRLNLRSIKCLKEPRVRV
WQAQWPLEPALSAAKMPRAAHAHAPHAFEIQASVPAGLQGSGYFAPSVRSDLRLPRSF
LFLNKK"
repeat_region 968991..969301
/note="Ty3 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007179"
repeat_region 969293..969431
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007175"
mobile_element 970286..976244
/note="YORWTy2-2; Ty2 element, LTR retrotransposon of the
Copia (Pseudoviridae) group; contains co-transcribed genes
TYA Gag and TYB Pol, encoding proteins involved in
structure and function of virus-like particles, flanked by
two direct repeats"
/mobile_element_type="retrotransposon:YORWTy2-2"
/db_xref="SGD:S000007183"
repeat_region 970286..970617
/note="Ty2 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007176"
gene <970578..>975891
/locus_tag="YOR343W-B"
/gene_synonym="YOR343C-B"
/db_xref="GeneID:854523"
mRNA <970578..>975891
/locus_tag="YOR343W-B"
/gene_synonym="YOR343C-B"
/product="gag-pol fusion protein"
/transcript_id="NM_001184390.4"
/db_xref="GeneID:854523"
CDS join(970578..971871,971873..975891)
/locus_tag="YOR343W-B"
/gene_synonym="YOR343C-B"
/EC_number="2.7.7.7"
/EC_number="2.7.7.49"
/EC_number="3.1.26.4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9448009]"
/ribosomal_slippage
/note="Retrotransposon TYA Gag and TYB Pol genes;
transcribed/translated as one unit; polyprotein is
processed to make a nucleocapsid-like protein (Gag),
reverse transcriptase (RT), protease (PR), and integrase
(IN); similar to retroviral genes"
/codon_start=1
/product="gag-pol fusion protein"
/protein_id="NP_620389.3"
/db_xref="GeneID:854523"
/db_xref="SGD:S000007356"
/translation="MESQQLHQNPHCPHGSAYASVTSKEVPSNQDPLAVSASNLPEFD
RDSTKVNSQEETTPGTSAVPENHHHVSPQPASVPPPQNGQYQQHGMMTPNKAMASNWA
HYQQPSMMTCSHYQTSPAYYQPDPHYPLPQYIPPLSTSSPDPIDSQDQHSEVPQAKTK
VRNNVLPPHPHTSEENFSTWVKFYIRFLKNSNLGDIIPNDQGEIKRQMTYEEHAYIYN
TFQAFAPFHLLPTWVKQILEINYSDILTVLCKSVSKMQTNNQELKDWIALANLEYNGS
TSADTFEITVSTIIQRLKENNINVSDRLACQLILKGLSGDFKYLRNQYRTKTNMKLSQ
LFAEIQLIYDENKIMNLNKPSQYKQHSEYKNVSRTSPNTTNTKVTTRNYHRTNSSKPR
AAKAHNIATSSKFSRVNNDHINESTVSSQYLSDDNELSLSQQQKESKPTRTIDSNDEL
PDHLLIDSGASQTLVRSAHYLHHATPNSEINIVDAQKQDIPINAIGNLHFNFQNGTKT
SIKALHTPNIAYDLLSLSELTNQNITACFTRNTLERSDGTVLAPIVKHGDFYWLSKKY
LIPSHISKLTINNVNKSKSVNKYPYPLIHRMLGHANFRSIQKSLKKNAVTYLKESDIE
WSNASTYQCPDCLIGKSTKHRHVKGSRLKYQESYEPFQYLHTDIFGPVHHLPKSAPSY
FISFTDEKTRFQWVYPLHDRREESILNVFTSILAFIKNQFNARVLVIQMDRGSEYTNK
TLHKFFTNRGITACYTTTADSRAHGVAERLNRTLLNDCRTLLHCSGLPNHLWFSAVEF
STIIRNSLVSPKNDKSARQHAGLAGLDITTILPFGQPVIVNNHNPDSKIHPRGIPGYA
LHPSRNSYGYIIYLPSLKKTVDTTNYVILQNNQTKLDQFDYDTLTFDDDLNRLTAHNQ
SFIEQNETEQSYDQNTESDHDYQSEIEINSDPLVNDFSSQSLNPLQLDKEPVQKVRAP
KEVDADISEYNILPSTIRSRTPHIINKESTEMGGTIESDTTSPRHSSTFTARNQKRPG
SPNDMIDLTSQDRVNYGLENIKTTRLGGTEEPYIQRNSDTNIKYRTTNSTPSIDDRSS
NSDSTTPIISIETKAACDNTPSIDTDPPEYRSSDHATPNIMPDKSSKNVTADSILDDL
PLPDLTHKSPTDTSDVSKDIPHIHSRQTNSSLGGMDDSNVLTTTKSKKRSLEDNETEI
EVSRDTWNNKNMRSLEPPRSKKRINLIAAIKGVKSIKPVRTTLRYDEAITYNKDNKEK
DRYVEAYHKEISQLLKMNTWDTNKYYDRNDIDPKKVINSMFIFNKKRDGTHKARFVAR
GDIQHPDTYDSDMQSNTVHHYALMTSLSIALDNDYYITQLDISSAYLYADIKEELYIR
PPPHLGLNDKLLRLRKSLYGLKQSGANWYETIKSYLINCCDMQEVRGWSCVFKNSQVT
ICLFVDDMILFSKDLNANKKIITTLKKQYDTKIINLGEGDNEIQYDILGLEIKYQRSK
YMKLGMEKSLTEKLPKLNVPLNPKGKKLRAPGQPGHYIDQDELEIDEDEYKEKVHEMQ
KLIGLASYVGYKFRFDLLYYINTLAQHILFPSRQVLDMTYELIQFMWDTRDKQLIWHK
NKPTKPDNKLVAISDASYGNQPYYKSQIGNIFLLNGKVIGGKSTKASLTCTSTTEAEI
HAVSEAIPLLNNLSHLVQELNKKPIIKGLLTDSRSTISIIKSTNEEKFRNRFFGTKAM
RLRDEVSGNNLYVYYIETKKNIADVMTKPLPIKTFKLLTNKWIH"
gene <970578..>971894
/locus_tag="YOR343W-A"
/gene_synonym="YOR343C-A"
/db_xref="GeneID:854522"
mRNA <970578..>971894
/locus_tag="YOR343W-A"
/gene_synonym="YOR343C-A"
/product="gag protein"
/transcript_id="NM_001184389.1"
/db_xref="GeneID:854522"
CDS 970578..971894
/locus_tag="YOR343W-A"
/gene_synonym="YOR343C-A"
/note="Retrotransposon TYA Gag gene co-transcribed with
TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is
a nucleocapsid protein that is the structural constituent
of virus-like particles (VLPs); similar to retroviral Gag"
/codon_start=1
/product="gag protein"
/protein_id="NP_620390.1"
/db_xref="GeneID:854522"
/db_xref="SGD:S000007355"
/translation="MESQQLHQNPHCPHGSAYASVTSKEVPSNQDPLAVSASNLPEFD
RDSTKVNSQEETTPGTSAVPENHHHVSPQPASVPPPQNGQYQQHGMMTPNKAMASNWA
HYQQPSMMTCSHYQTSPAYYQPDPHYPLPQYIPPLSTSSPDPIDSQDQHSEVPQAKTK
VRNNVLPPHPHTSEENFSTWVKFYIRFLKNSNLGDIIPNDQGEIKRQMTYEEHAYIYN
TFQAFAPFHLLPTWVKQILEINYSDILTVLCKSVSKMQTNNQELKDWIALANLEYNGS
TSADTFEITVSTIIQRLKENNINVSDRLACQLILKGLSGDFKYLRNQYRTKTNMKLSQ
LFAEIQLIYDENKIMNLNKPSQYKQHSEYKNVSRTSPNTTNTKVTTRNYHRTNSSKPR
AAKAHNIATSSKFSRVNNDHINESTVSSQYLSDDNELSLRPATERI"
repeat_region 975913..976244
/note="Ty2 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007177"
gene 976421..976493
/gene="EMT2"
/locus_tag="YNCO0030W"
/db_xref="GeneID:854524"
tRNA 976421..976493
/gene="EMT2"
/locus_tag="YNCO0030W"
/product="tRNA-Met"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Methionine tRNA (tRNA-Met), functions in
translational elongation; not involved in translational
initiation; predicted by tRNAscan-SE analysis"
/db_xref="GeneID:854524"
/db_xref="SGD:S000006666"
gene complement(<977194..>978069)
/gene="TYE7"
/locus_tag="YOR344C"
/gene_synonym="SGC1"
/db_xref="GeneID:854525"
mRNA complement(<977194..>978069)
/gene="TYE7"
/locus_tag="YOR344C"
/gene_synonym="SGC1"
/product="Tye7p"
/transcript_id="NM_001183764.3"
/db_xref="GeneID:854525"
CDS complement(977194..978069)
/gene="TYE7"
/locus_tag="YOR344C"
/gene_synonym="SGC1"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:12464632]"
/experiment="EXISTENCE:direct assay:GO:0003700 DNA-binding
transcription factor activity [PMID:10606743]"
/experiment="EXISTENCE:direct assay:GO:0006368
transcription elongation by RNA polymerase II
[PMID:10606743]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:19158363]"
/experiment="EXISTENCE:genetic interaction:GO:0045821
positive regulation of glycolytic process [PMID:10606743]"
/experiment="EXISTENCE:mutant phenotype:GO:0045821
positive regulation of glycolytic process [PMID:10606743]"
/note="Serine-rich protein that contains a bHLH DNA
binding motif; binds E-boxes of glycolytic genes and
contributes to their activation; may function as a
transcriptional activator in Ty1-mediated gene expression;
bHLH stands for basic-helix-loop-helix"
/codon_start=1
/product="Tye7p"
/protein_id="NP_014989.3"
/db_xref="GeneID:854525"
/db_xref="SGD:S000005871"
/translation="MNSILDRNVRSSETTLIKPESEFDNWLSDENDGASHINVNKDSS
SVLSASSSTWFEPLENIISSASSSSIGSPIEDQFISSNNEESALFPTDQFFSNPSSYS
HSPEVSSSIKREEDDNALSLADFEPASLQLMPNMINTDNNDDSTPLKNEIELNDSFIK
TNLDAKETKKRAPRKRLTPFQKQAHNKIEKRYRININTKIARLQQIIPWVASEQTAFE
VGDSVKKQDEDGAETAATTPLPSAAATSTKLNKSMILEKAVDYILYLQNNERLYEMEV
QRLKSEIDTLKQDQK"
repeat_region complement(980259..980590)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007167"
gene 980683..980787
/locus_tag="YNCO0031W"
/db_xref="GeneID:854526"
tRNA join(980683..980718,980752..980787)
/locus_tag="YNCO0031W"
/product="tRNA-Pro"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE
analysis; target of K. lactis zymocin"
/db_xref="GeneID:854526"
/db_xref="SGD:S000006689"
rep_origin 981462..981697
/note="ARS1529; Autonomously Replicating Sequence"
/db_xref="SGD:S000118496"
gene <981828..>984785
/gene="REV1"
/locus_tag="YOR346W"
/db_xref="GeneID:854527"
mRNA <981828..>984785
/gene="REV1"
/locus_tag="YOR346W"
/product="deoxycytidyl transferase"
/transcript_id="NM_001183766.1"
/db_xref="GeneID:854527"
CDS 981828..984785
/gene="REV1"
/locus_tag="YOR346W"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:16546083]"
/experiment="EXISTENCE:direct assay:GO:0003887
DNA-directed DNA polymerase activity [PMID:8751446]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16452144]"
/experiment="EXISTENCE:direct assay:GO:0017125
deoxycytidyl transferase activity [PMID:8751446]"
/experiment="EXISTENCE:direct assay:GO:0042276 error-prone
translesion synthesis [PMID:8751446]"
/experiment="EXISTENCE:direct assay:GO:0070987 error-free
translesion synthesis [PMID:8751446]"
/experiment="EXISTENCE:genetic interaction:GO:0042276
error-prone translesion synthesis [PMID:9765213]"
/experiment="EXISTENCE:mutant phenotype:GO:0042276
error-prone translesion synthesis [PMID:17248528]"
/experiment="EXISTENCE:mutant phenotype:GO:0070987
error-free translesion synthesis [PMID:10540291]"
/experiment="EXISTENCE:physical interaction:GO:0005657
replication fork [PMID:17517887]"
/note="Bifunctional DNA-directed DNA
polymerase/deoxycytidyl transferase; involved in repair of
abasic sites and adducted guanines in damaged DNA by
translesion synthesis (TLS); forms a complex with the
subunits of DNA polymerase zeta, Rev3p and Rev7p;
relocalizes from nucleus to cytoplasm upon DNA replication
stress; may be involved in meiosis"
/codon_start=1
/product="deoxycytidyl transferase"
/protein_id="NP_014991.1"
/db_xref="GeneID:854527"
/db_xref="SGD:S000005873"
/translation="MGEHGGLVDLLDSDLEYSINRETPDKNNCLSQQSVNDSHLTAKT
GGLNARSFLSTLSDDSLIEYVNQLSQTNKNNSNPTAGTLRFTTKNISCDELHADLGGG
EDSPIARSVIEIQESDSNGDDVKKNTVYTREAYFHEKAHGQTLQDQILKDQYKDQISS
QSSKIFKNCVIYINGYTKPGRLQLHEMIVLHGGKFLHYLSSKKTVTHIVASNLPLKKR
IEFANYKVVSPDWIVDSVKEARLLPWQNYSLTSKLDEQQKKLDNCKTVNSIPLPSETS
LHKGSKCVGSALLPVEQQSPVNLNNLEAKRIVACDDPDFLTSYFAHSRLHHLSAWKAN
LKDKFLNENIHKYTKITDKDTYIIFHIDFDCFFATVAYLCRSSSFSACDFKRDPIVVC
HGTKNSDIASCNYVARSYGIKNGMWVSQAEKMLPNGIKLISLPYTFEQFQLKSEAFYS
TLKRLNIFNLILPISIDEAVCVRIIPDNIHNTNTLNARLCEEIRQEIFQGTNGCTVSI
GCSDSLVLARLALKMAKPNGYNITFKSNLSEEFWSSFKLDDLPGVGHSTLSRLESTFD
SPHSLNDLRKRYTLDALKASVGSKLGMKIHLALQGQDDEESLKILYDPKEVLQRKSLS
IDINWGIRFKNITQVDLFIERGCQYLLEKLNEINKTTSQITLKLMRRCKDAPIEPPKY
MGMGRCDSFSRSSRLGIPTNEFGIIATEMKSLYRTLGCPPMELRGLALQFNKLVDVGP
DNNQLKLRLPFKTIVTNRAFEALPEDVKNDINNEFEKRNYKRKESGLTSNSLSSKKKG
FAISRLEVNDLPSTMEEQFMNELPTQIRAEVRHDLRIQKKIQQTKLGNLQEKIKRREE
SLQNEKNHFMGQNSIFQPIKFQNLTRFKKICQLVKQWVAETLGDGGPHEKDVKLFVKY
LIKLCDSNRVHLVLHLSNLISRELNLCAFLNQDHSGFQTWERILLNDIIPLLNRNKHT
YQTVRKLDMDFEV"
gene complement(<984942..>986462)
/gene="PYK2"
/locus_tag="YOR347C"
/db_xref="GeneID:854529"
mRNA complement(<984942..>986462)
/gene="PYK2"
/locus_tag="YOR347C"
/product="pyruvate kinase PYK2"
/transcript_id="NM_001183767.3"
/db_xref="GeneID:854529"
CDS complement(984942..986462)
/gene="PYK2"
/locus_tag="YOR347C"
/EC_number="2.7.1.40"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16962558]"
/experiment="EXISTENCE:direct assay:GO:1904408 melatonin
binding [PMID:31708896]"
/experiment="EXISTENCE:mutant phenotype:GO:0004743
pyruvate kinase activity [PMID:9139918]"
/experiment="EXISTENCE:mutant phenotype:GO:0006090
pyruvate metabolic process [PMID:9139918]"
/note="Pyruvate kinase; appears to be modulated by
phosphorylation; transcription repressed by glucose, and
Pyk2p may be active under low glycolytic flux; PYK2 has a
paralog, CDC19, that arose from the whole genome
duplication"
/codon_start=1
/product="pyruvate kinase PYK2"
/protein_id="NP_014992.3"
/db_xref="GeneID:854529"
/db_xref="SGD:S000005874"
/translation="MPESRLQRLANLKIGTPQQLRRTSIIGTIGPKTNSCEAITALRK
AGLNIIRLNFSHGSYEFHQSVIENAVKSEQQFPGRPLAIALDTKGPEIRTGRTLNDQD
LYIPVDHQMIFTTDASFANTSNDKIMYIDYANLTKVIVPGRFIYVDDGILSFKVLQII
DESNLRVQAVNSGYIASHKGVNLPNTDVDLPPLSAKDMKDLQFGVRNGIHIVFASFIR
TSEDVLSIRKALGSEGQDIKIISKIENQQGLDNFDEILEVTDGVMIARGDLGIEILAP
EVLAIQKKLIAKCNLAGKPVICATQMLDSMTHNPRPTRAEVSDVGNAVLDGADCVMLS
GETAKGDYPVNAVNIMAATALIAESTIAHLALYDDLRDATPKPTSTTETVAAAATAAI
LEQDGKAIVVLSTTGNTARLLSKYRPSCPIILVTRHARTARIAHLYRGVFPFLYEPKR
LDDWGEDVHRRLKFGVEMARSFGMVDNGDTVVSIQGFKGGVGHSNTLRISTVGQEF"
gene complement(<986899..>988782)
/gene="PUT4"
/locus_tag="YOR348C"
/db_xref="GeneID:854530"
mRNA complement(<986899..>988782)
/gene="PUT4"
/locus_tag="YOR348C"
/product="proline permease PUT4"
/transcript_id="NM_001183768.1"
/db_xref="GeneID:854530"
CDS complement(986899..988782)
/gene="PUT4"
/locus_tag="YOR348C"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:28679684]"
/experiment="EXISTENCE:direct assay:GO:0015175 neutral
L-amino acid transmembrane transporter activity
[PMID:10654085]"
/experiment="EXISTENCE:direct assay:GO:0015804 neutral
amino acid transport [PMID:10654085]"
/experiment="EXISTENCE:direct assay:GO:0015812
gamma-aminobutyric acid transport [PMID:8455553]"
/experiment="EXISTENCE:direct assay:GO:0015824 proline
transport [PMID:10654085]"
/experiment="EXISTENCE:genetic interaction:GO:0015193
L-proline transmembrane transporter activity
[PMID:14968425]"
/experiment="EXISTENCE:genetic interaction:GO:0015812
gamma-aminobutyric acid transport [PMID:8455553]"
/experiment="EXISTENCE:mutant phenotype:GO:0015193
L-proline transmembrane transporter activity
[PMID:3552672]"
/note="Proline permease; required for high-affinity
transport of proline; also transports the toxic proline
analog azetidine-2-carboxylate (AzC); PUT4 transcription
is repressed in ammonia-grown cells"
/codon_start=1
/product="proline permease PUT4"
/protein_id="NP_014993.1"
/db_xref="GeneID:854530"
/db_xref="SGD:S000005875"
/translation="MVNILPFHKNNRHSAGVVTCADDVSGDGSGGDTKKEEDVVQVTE
SPSSGSRNNHRSDNEKDDAIRMEKISKNQSASSNGTIREDLIMDVDLEKSPSVDGDSE
PHKLKQGLQSRHVQLIALGGAIGTGLLVGTSSTLHTCGPAGLFISYIIISAVIYPIMC
ALGEMVCFLPGDGSDSAGSTANLVTRYVDPSLGFATGWNYFYCYVILVAAECTAASGV
VEYWTTAVPKGVWITIFLCVVVILNFSAVKVYGESEFWFASIKILCIVGLIILSFILF
WGGGPNHDRLGFRYWQHPGAFAHHLTGGSLGNFTDIYTGIIKGAFAFILGPELVCMTS
AECADQRRNIAKASRRFVWRLIFFYVLGTLAISVIVPYNDPTLVNALAQGKPGAGSSP
FVIGIQNAGIKVLPHIINGCILTSAWSAANAFMFASTRSLLTMAQTGQAPKCLGRINK
WGVPYVAVGVSFLCSCLAYLNVSSSTADVFNWFSNISTISGFLGWMCGCIAYLRFRKA
IFYNGLYDRLPFKTWGQPYTVWFSLIVIGIITITNGYAIFIPKYWRVADFIAAYITLP
IFLVLWFGHKLYTRTWRQWWLPVSEIDVTTGLVEIEEKSREIEEMRLPPTGFKDKFLD
ALL"
gene <989789..>992833
/gene="CIN1"
/locus_tag="YOR349W"
/db_xref="GeneID:854531"
mRNA <989789..>992833
/gene="CIN1"
/locus_tag="YOR349W"
/product="Cin1p"
/transcript_id="NM_001183769.3"
/db_xref="GeneID:854531"
CDS 989789..992833
/gene="CIN1"
/locus_tag="YOR349W"
/experiment="EXISTENCE:genetic interaction:GO:0006457
protein folding [PMID:2407611]"
/experiment="EXISTENCE:genetic interaction:GO:0007021
tubulin complex assembly [PMID:9215891]"
/experiment="EXISTENCE:mutant phenotype:GO:0007021 tubulin
complex assembly [PMID:9215891]"
/note="Tubulin folding factor D involved in beta-tubulin
(Tub2p) folding; isolated as mutant with increased
chromosome loss and sensitivity to benomyl; CIN1 mRNA
level is reduced when downstream gene MNE1 is deleted"
/codon_start=1
/product="Cin1p"
/protein_id="NP_014994.3"
/db_xref="GeneID:854531"
/db_xref="SGD:S000005876"
/translation="MNNIRALLDSIQSGVQTVSPEKHQQTIAAINKFQDDPALLDTIL
PRCVPLLTKSFFCMSQRDQKLVAELFYNLDKISHSKVLKSLDTSIFRLNEILNYLQDR
ASPSSFSDVLCVYLNLSWLSVILLSPYAFKDKFNKTLQVSSRFENYPICIPPINKIKA
VLYFKNFTDAFDQLPEREQANVPFLNQFLKLFIQSSEKANYYFSNENLRHLQQVALSN
DGIKLLPKLFQISFNHGSHDILDAIVEFFHDHLSSNSTDTRFQLAHSFAKIAKFLHQA
DPASFIELIDYTIENTVSLLQAPCDSIDSNELHTSLLIIAEVALAKILPIDLVDRVLT
LIIPKTCHFQKSHFQIIKGHHIRDSTNFIIWSVIRSNRSNSLSPQVLQSLLSHLLINA
FFDPELIIRYSSFAALQELLGRSNKSLALNQNDIALILQANWKDLPRSFEENSGLIRR
LFNPENTSKSAVCVWKVFRDWSFNWNLLENLHLTTMKLNIDYNLVPLIKSKLSSPALL
QEVLNKAGSSVTQNCQILYLYLKLFENDVNCPKISEICIDIYQKKIKFQLTTQAKRQF
NDNSPELFQIFVILKYWQLTGQNDFNQELFWKFVDIVSPQKKLNLYNEFIPIIQQIIS
QCVSLNYTRIVQLIKSDNELTCRSICHMPDQEKMCSLFFSQFPLLSPQSRSLLIGELD
HHWDVRISLLPSNSYRKFRNIIINCLDDYTITQQGDVGRLVRIQALKLMQSHPDFLSG
DCDSINPKLTRLLAEPVPEIRKLSYQLLASATSQITVLSDSSILNFRHKQGLSEEFWK
GYAVSAGAIHFTDSQLTSSIDSFIVYYRSLSPSQQLELCHDLIRIIPSAKQIAESRIR
DRNKDPLTGGMRFDTIKFTIHCVKFWTRIMESGLVVLHPNFNFQGVFAKFYNLHLLDC
TTLRVSVIKFFPFLAISCYHTMRENADQKNLSNIILKRLLVLVKREYAATKSKFMTDQ
NVALQGMFQIFLELGVTRQLQALQVACQKHELANILESDITL"
gene complement(<992864..>994855)
/gene="MNE1"
/locus_tag="YOR350C"
/db_xref="GeneID:854532"
mRNA complement(<992864..>994855)
/gene="MNE1"
/locus_tag="YOR350C"
/product="Mne1p"
/transcript_id="NM_001183770.1"
/db_xref="GeneID:854532"
CDS complement(992864..994855)
/gene="MNE1"
/locus_tag="YOR350C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:21257754]"
/experiment="EXISTENCE:direct assay:GO:1990904
ribonucleoprotein complex [PMID:21257754]"
/experiment="EXISTENCE:mutant phenotype:GO:0000372 Group I
intron splicing [PMID:21257754]"
/experiment="EXISTENCE:mutant phenotype:GO:0090615
mitochondrial mRNA processing [PMID:21257754]"
/note="Protein involved in splicing Group I aI5-beta
intron from COX1 mRNA; mitochondrial matrix protein"
/codon_start=1
/product="Mne1p"
/protein_id="NP_014995.1"
/db_xref="GeneID:854532"
/db_xref="SGD:S000005877"
/translation="MKLLFKRYSSSHIGKLIKDSLITPEILPQLGRQPSSHKRLPNNK
RTNSITDKWLKDALTRKDKLNEDKLQNVNLRLNVVLTTLQKLRTSDNPALYFALLNRI
GTGHIKWLNKSGRQIDAFPPDRLPLEFYHELSNMLYKLSLRSANDKIALAKFSLQLLD
RYYFLKTKSLTIEGKFRANIKFLRNCTLLIVKSQSNYYLRAIQRLFAENSEGQLLANL
SQLAFYVETSQWTSMLDILSSCVPDSGLRGSKERERAIQLLELFSPCLVKSLKVMIAQ
NMENEACQILRSLSEWNFHFDQHDSSNLIQLSQNHSCLKVIETMNGLSSTTAVTRQFG
LEKLPTDVSLKQSIHILSKDNFEPLKQDSFLQFLSFKLSDLPLNLEVWKKHIKEVDDQ
MQAESNLHSLRAFFIDMLLCHLSVRKDFDFMLSLVEHIVYEKNLWQPLLLTDNIVGNK
ENSTFHCLFHGASQDISTKLTLLALYNQLNEIGYQFTSHDFLSMLKVCKNYSDSDFFY
FAFYNLLVTQSHKFFLFDKFSDKFSWRLPIQIGDAISEWLSSLEIDIQENTDRVLQIT
DDVGEWYVENKPFKSEKGTIQPINIMELRKIFGERKTLFHMDSEIFQKSKAKRDKEMR
NEALFTANDAEYNFAADVSYAKRVENLFSYIRSKQMQQK"
gene complement(<995018..>996511)
/gene="MEK1"
/locus_tag="YOR351C"
/gene_synonym="MRE4"
/db_xref="GeneID:854533"
mRNA complement(<995018..>996511)
/gene="MEK1"
/locus_tag="YOR351C"
/gene_synonym="MRE4"
/product="serine/threonine protein kinase MEK1"
/transcript_id="NM_001183771.3"
/db_xref="GeneID:854533"
CDS complement(995018..996511)
/gene="MEK1"
/locus_tag="YOR351C"
/gene_synonym="MRE4"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0004674 protein
serine/threonine kinase activity
[PMID:9832507|PMID:27483004]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:24390141|PMID:7926789]"
/experiment="EXISTENCE:mutant phenotype:GO:0004674 protein
serine/threonine kinase activity [PMID:10786836]"
/experiment="EXISTENCE:mutant phenotype:GO:0051321 meiotic
cell cycle [PMID:1752435]"
/experiment="EXISTENCE:mutant phenotype:GO:0051598 meiotic
recombination checkpoint signaling [PMID:10848609]"
/note="Meiosis-specific serine/threonine protein kinase;
functions in meiotic checkpoint, promotes recombination
between homologous chromosomes by suppressing double
strand break repair between sister chromatids; stabilizes
Hop1-Thr318 phosphorylation to promote interhomolog
recombination and checkpoint responses during meiosis"
/codon_start=1
/product="serine/threonine protein kinase MEK1"
/protein_id="NP_014996.3"
/db_xref="GeneID:854533"
/db_xref="SGD:S000005878"
/translation="MRPLYSCNLATKDDIEMAGGVAPAHLEVNVGGYNTEQTIPIVKH
QLVKVGRNDKECQLVLTNPSISSVHCVFWCVFFDEDSIPMFYVKDCSLNGTYLNGLLL
KRDKTYLLKHCDVIELSQGSEENDIKKTRLVFMINDDLQSSLDPKLLDQMGFLREVDQ
WEITNRIVGNGTFGHVLITHNSKERDEDVCYHPENYAVKIIKLKPNKFDKEARILLRL
DHPNIIKVYHTFCDRNNHLYIFQDLIPGGDLFSYLAKGDCLTSMSETESLLIVFQILQ
ALNYLHDQDIVHRDLKLDNILLCTPEPCTRIVLADFGIAKDLNSNKERMHTVVGTPEY
CAPEVGFRANRKAYQSFSRAATLEQRGYDSKCDLWSLGVITHIMLTGISPFYGDGSER
SIIQNAKIGKLNFKLKQWDIVSDNAKSFVKDLLQTDVVKRLNSKQGLKHIWIAKHLSQ
LERLYYKKILCNNEGPKLESINSDWKRKLPKSVIISQAIPKKKKVLE"
gene <997213..>998244
/gene="TFB6"
/locus_tag="YOR352W"
/db_xref="GeneID:854534"
mRNA <997213..>998244
/gene="TFB6"
/locus_tag="YOR352W"
/product="TFIIH complex subunit TFB6"
/transcript_id="NM_001183772.1"
/db_xref="GeneID:854534"
CDS 997213..998244
/gene="TFB6"
/locus_tag="YOR352W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005675
transcription factor TFIIH holo complex
[PMID:22411836|PMID:27381459]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="Subunit of TFIIH complex; facilities dissociation
of the Ssl2p helices from TFIIH; expression levels
regulated by Arg5,6p; green fluorescent protein
(GFP)-fusion protein localizes to the cytoplasm and
nucleus"
/codon_start=1
/product="TFIIH complex subunit TFB6"
/protein_id="NP_014997.1"
/db_xref="GeneID:854534"
/db_xref="SGD:S000005879"
/translation="MSEPNTPLHAQPNEQLDLNNLNDLDEKDIDDLNLDPNSDVEISA
DSGDVVNSNIDNVIWQRNCNKKRRYHTPEFNDVYNETNNTINDVTMLDDVDDFQPRIN
VSSPFSSATKLSELLPNDHNGTSHPRRLSMSQQSKFISYVDDQLLQIQRKFVQSRGLN
IKNGYASLTPLLQDLKTLVDFVWYSIAHVPNSDYLLQSEEKRHCPDSRNPKDTCGYSS
YFGQGSYLIKIADDLIDYVEKFTFKNMEDSEINDTLSKLFKLFFILDRIFVILTDDND
NCKEVPKTSSASKNIAGLNGTDIVRLKGIAERTRVRLPIFLESQGIHGYHYELSKIYE
GFLDHANSF"
gene complement(<998453..>1000828)
/gene="SOG2"
/locus_tag="YOR353C"
/db_xref="GeneID:854535"
mRNA complement(<998453..>1000828)
/gene="SOG2"
/locus_tag="YOR353C"
/product="Sog2p"
/transcript_id="NM_001183773.3"
/db_xref="GeneID:854535"
CDS complement(998453..1000828)
/gene="SOG2"
/locus_tag="YOR353C"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:12972564]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005933 cellular
bud [PMID:12972564]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:12972564]"
/experiment="EXISTENCE:mutant phenotype:GO:0000920 septum
digestion after cytokinesis [PMID:12972564]"
/experiment="EXISTENCE:mutant phenotype:GO:0007118 budding
cell apical bud growth [PMID:12972564]"
/experiment="EXISTENCE:mutant phenotype:GO:0007165 signal
transduction [PMID:12972564]"
/note="Key component of the RAM signaling network;
required for proper cell morphogenesis and cell separation
after mitosis"
/codon_start=1
/product="Sog2p"
/protein_id="NP_014998.3"
/db_xref="GeneID:854535"
/db_xref="SGD:S000005880"
/translation="MVATSSKRTLDPKEEHLPADKTSTNSSNTIISELATQEKSSSSG
TTLKLIALNIKSISDEDVGYIQNVERLSLRKNHLTSLPASFKRLSRLQYLDLHNNNFK
EIPYILTQCPQLEILDLSSNEIEALPDEISSFWQDNIRVLSLKDNNVTSIRNLKSITK
LNKLSILDLEDNKIPKEELDQVQSYTPFHTGIPKEEYWAIAISRYLKDHPNLPTPEPK
ISRAAKRMGFINTNLSNGAMNENNIISLAPSANTTISASTAMVSSNQTSATSFSGTVN
AESEQSGAVNGTELYNHTKYNDYFKRLSILPEESMSNGHQKISHAELVVSCRKLLFSF
TECQQAIRKIASFCKEKAVAVNVVSLLYSVRSHTDNLVEVLQQTENEDESHDQALIKL
CLTIITNFKQIITLLRKNFEIFFKEDDLCFIRMFYMTLMCAYMEMYNAWSFIKEDDQV
SGSASKAPKKHSFSRHETSSSSITSGGGPAASTTSTHCSGNIKLLPKTRSTRTPSASA
LLSNSNILTGDTTAVPLLSPNLNGAHTHGPILGHQNAISNGSSQTNMNEVKTTSDTIP
RQQLLQHNKSISDSKKESQAHEPKQHPVMTSSIINASNSNNVSNVNITPPPMNGGGAA
NSSANVVETNIDIQLYQTLSTVVKMVSVVYNQLTSEISKIAIASTMGKQILTDSLAPK
IRDLTETCRQAMDLSKQLNERLNVLIPNDSNSEKYLTSLEKLKTWEIMNSFLKVIISI
LANTKIVMSDVPNLNELRPNLANLAKITKDVTVILDLSSYKAVSVSANSPE"
gene complement(<1001147..>1003225)
/gene="MSC6"
/locus_tag="YOR354C"
/db_xref="GeneID:854536"
mRNA complement(<1001147..>1003225)
/gene="MSC6"
/locus_tag="YOR354C"
/product="Msc6p"
/transcript_id="NM_001183774.1"
/db_xref="GeneID:854536"
CDS complement(1001147..1003225)
/gene="MSC6"
/locus_tag="YOR354C"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:14576278|PMID:14562095|PMID:16823961|PMID:24769239|P
MID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:26780366]"
/experiment="EXISTENCE:mutant phenotype:GO:0007131
reciprocal meiotic recombination [PMID:10511544]"
/experiment="EXISTENCE:mutant phenotype:GO:0070124
mitochondrial translational initiation [PMID:30767393]"
/note="Multicopy suppressor of HER2 involved in
mitochondrial translation; mutant is defective in
directing meiotic recombination events to homologous
chromatids"
/codon_start=1
/product="Msc6p"
/protein_id="NP_014999.1"
/db_xref="GeneID:854536"
/db_xref="SGD:S000005881"
/translation="MLSHNALRAFDCSKVIISRRCLTSSTSIYQQSSVHLQETDDGHS
GNREKHVSPFERVQNLAADLKNELKAPDSDINEVFNDFKDKIESLKQKLRNPSPMERS
HLLANFSSDLLQELSYRSKNMTLDPYQVLNTLCQYKLARSQHFTIVLKYLLYNQSPQD
VIALWVKYLETISENPVILLQNSSSRAHMQNIAITTIAYLSLPENTVDINILYKILQI
DRKMGQVLPFNMIRRMLSTEFSSLERRDVIIKNLNTLYYQYTVQDSDHFLSQIENAPR
WIDLRDLYGQYNKLEGEKNVEIISKFMDKFIDLDKPDQVVTIYNQYSKVFPNSTSLKD
CLLRAVSHLRAKSSKEKLDRILAVWNSVIKPGDNIKNTSYATLVNALTDSGNFNHLKE
FWEEELPKKFKKDPIVKEAFLLALCQTSPLKYDQVKGELAETVKTKKLFNKVLLLMLD
DEKVSEEQFNTFYYNHYPSDGVLPPTLDTLSIKMYANYKFQAEDTRPQFDLLQSVSIN
PTDYEKVEKITKAFISVCPTVEPIRQLYKQLGTHLNARNYADFISAEFNKPDGTVAEA
KNLFSDFLSYQKTRKRNVDNTPLNALLLGFCDKLYKSKHSEYVPYIEKYYNLAKDSSI
RVSNLAVSKILFNLATFARNTQQLSDKEVAFINQFMRDLGTNEGFRPNPKDIQILKEC
DGITVPEKLT"
gene <1005137..>1006705
/gene="GDS1"
/locus_tag="YOR355W"
/db_xref="GeneID:854537"
mRNA <1005137..>1006705
/gene="GDS1"
/locus_tag="YOR355W"
/product="Gds1p"
/transcript_id="NM_001183775.3"
/db_xref="GeneID:854537"
CDS 1005137..1006705
/gene="GDS1"
/locus_tag="YOR355W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:34694912|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0062060 NuA4
histone acetyltransferase complex binding [PMID:34694912]"
/experiment="EXISTENCE:mutant phenotype:GO:0009060 aerobic
respiration [PMID:8750239]"
/note="Protein involved in histone H4 acetylation at
ribosomal protein genes; interacts with and modulates NuA4
histone acetyltransferase complex; required for growth on
glycerol as a carbon source; the authentic, non-tagged
protein is detected in highly purified mitochondria in
high-throughput studies; contains a segment predicted to
resemble the winged-helix/forkhead domain located in
DNA-binding proteins"
/codon_start=1
/product="Gds1p"
/protein_id="NP_015000.3"
/db_xref="GeneID:854537"
/db_xref="SGD:S000005882"
/translation="MALANSRPLQIPTLENEILHNSNSPVFQLNSMGFTTRADTISNP
GTDLIGNQPGMALDDNNLAGSSFSSSQEIKATKPKKDFGAPKKDNPLLEISKLIPVTG
ERPKPENRDSPLDDDVLHAVFLILWEMDPNQQGMTVKQLCDLLLQKHPDMSNLSTKLS
NLISAKLNAYVKKIEKGEKTLTYALSREWSNSSPRRMLYIYRGILSPDYKEHAQAVTM
QLKQQLETSGDTSDFNSNGKKKRESSSNQLVNNDSYSSSMTDMKNMSSNSSFSKNLNV
GNLAFSLSPEFNIPYSTSPVSLNLSPSMSNNQQQLLTPNSASKSKNNNKKRNYMDEDT
NESMTEPKKTKTTKPGKQTKSQSLSVLSTPKKGSSASLSTFASSKNISPDSSLSHNAS
SNTYVTAAAAAPRLSKLLPKNGFKKNSRSSSELAAIHKVISTQTPIESSSESSQYNSS
SSSPVNSAAASSAESLSDINSSQDNGRESNPSSQESRNEVTNWMKIVRNGFLTHDIES
PESITLDDLENIFN"
gene <1007221..>1009116
/gene="CIR2"
/locus_tag="YOR356W"
/db_xref="GeneID:854538"
mRNA <1007221..>1009116
/gene="CIR2"
/locus_tag="YOR356W"
/product="electron-transferring-flavoprotein
dehydrogenase"
/transcript_id="NM_001183776.1"
/db_xref="GeneID:854538"
CDS 1007221..1009116
/gene="CIR2"
/locus_tag="YOR356W"
/EC_number="1.5.5.1"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:14576278|PMID:24769239|PMID:16823961|PMID:11502169]"
/note="Putative ortholog of human ETF-dH; found in a large
supramolecular complex with other mitochondrial
dehydrogenases; may have a role in oxidative stress
response; ETF-dH is also known as electron transfer
flavoprotein dehydrogenase"
/codon_start=1
/product="electron-transferring-flavoprotein
dehydrogenase"
/protein_id="NP_015001.1"
/db_xref="GeneID:854538"
/db_xref="SGD:S000005883"
/translation="MIKFTNENLIRGIRMTISAKSRHLALGTDMTRKFSLSCRFLNKA
NLTEEEKELLNEPRARDYVDVCIVGGGPAGLATAIKLKQLDNSSGTGQLRVVVLEKSS
VLGGQTVSGAILEPGVWKELFPDEKSDIGIPLPKELATLVTKEHLKFLKGKWAISVPE
PSQMINKGRNYIVSLNQVVGYLGEKAEEVGVEVYPGIAVSDLIYDENNAVKGVITKDA
GISKSGKPKETFERGMEFWARQTVLAEGCHGSLTKQALAKYDLRKGRQHQTYGLGIKE
VWEVKPENFNKGFAAHTMGYPLTNDVYGGGFQYHFGDGLVTVGLVVGLDYKNPYVSPY
KEFQKMKHHPYYSKVLEGGKCIAYAARALNEGGLQSVPKLNFPGGVLVGASAGFMNVP
KIKGTHTAMKSGLLAAESIFESIKGLPVLEEVEDEDAKMAMFDKEATINLESYESAFK
ESSIYKELYEVRNIRPSFSGKLGGYGGMIYSGIDSLILKGKVPWTLKFDEKNDGEILE
PASKYKPIEYPKPDGVISFDILTSVSRTGTYHDDDEPCHLRVPGQDMVKYAERSFPVW
KGVESRFCPAGVYEFVKDEKSPVGTRLQINSQNCIHCKTCDIKAPRQDITWKVPEGGD
GPKYTLT"
gene complement(<1009224..>1009712)
/gene="SNX3"
/locus_tag="YOR357C"
/gene_synonym="GRD19"
/db_xref="GeneID:854539"
mRNA complement(<1009224..>1009712)
/gene="SNX3"
/locus_tag="YOR357C"
/gene_synonym="GRD19"
/product="Snx3p"
/transcript_id="NM_001183777.3"
/db_xref="GeneID:854539"
CDS complement(1009224..1009712)
/gene="SNX3"
/locus_tag="YOR357C"
/gene_synonym="GRD19"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:17420293]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:9456318]"
/experiment="EXISTENCE:direct assay:GO:0032266
phosphatidylinositol-3-phosphate binding [PMID:11557775]"
/experiment="EXISTENCE:mutant phenotype:GO:0008104
intracellular protein localization [PMID:9456318]"
/experiment="EXISTENCE:mutant phenotype:GO:0034499 late
endosome to Golgi transport [PMID:12554655]"
/note="Sorting nexin for late-Golgi enzymes; required to
maintain late-Golgi resident enzymes in their proper
location by recycling molecules from the prevacuolar
compartment; contains a PX domain and sequence similarity
to human Snx3p"
/codon_start=1
/product="Snx3p"
/protein_id="NP_015002.3"
/db_xref="GeneID:854539"
/db_xref="SGD:S000005884"
/translation="MPREFKSFGSTEKSLLSKGHGEPSYSEIYAEPENFLEIEVHNPK
THIPNGMDSKGMFTDYEIICRTNLPSFHKRVSKVRRRYSDFEFFRKCLIKEISMLNHP
KVMVPHLPGKILLSNRFSNEVIEERRQGLNTWMQSVAGHPLLQSGSKVLVRFIEAEKF
VG"
gene <1010161..>1010889
/gene="HAP5"
/locus_tag="YOR358W"
/db_xref="GeneID:854540"
mRNA <1010161..>1010889
/gene="HAP5"
/locus_tag="YOR358W"
/product="Hap5p"
/transcript_id="NM_001183778.1"
/db_xref="GeneID:854540"
CDS 1010161..1010889
/gene="HAP5"
/locus_tag="YOR358W"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:7828851]"
/experiment="EXISTENCE:direct assay:GO:0016602
CCAAT-binding factor complex [PMID:7828851]"
/experiment="EXISTENCE:genetic interaction:GO:0043457
regulation of cellular respiration [PMID:7828851]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:12614847]"
/experiment="EXISTENCE:physical interaction:GO:0016602
CCAAT-binding factor complex [PMID:7828851]"
/note="Subunit of the Hap2p/3p/4p/5p CCAAT-binding
complex; complex is heme-activated and glucose repressed;
complex is a transcriptional activator and global
regulator of respiratory gene expression; required for
assembly and DNA binding activity of the complex"
/codon_start=1
/product="Hap5p"
/protein_id="NP_015003.1"
/db_xref="GeneID:854540"
/db_xref="SGD:S000005885"
/translation="MTDRNFSPQQGQGPQESLPEGPQPSTMIQREEMNMPRQYSEQQQ
LQENEGEGENTRLPVSEEEFRMVQELQAIQAGHDQANLPPSGRGSLEGEDNGNSDGAD
GEMDEDDEEYDVFRNVGQGLVGHYKEIMIRYWQELINEIESTNEPGSEHQDDFKSHSL
PFARIRKVMKTDEDVKMISAEAPIIFAKACEIFITELTMRAWCVAERNKRRTLQKADI
AEALQKSDMFDFLIDVVPRRPLPQ"
gene <1011189..>1012760
/gene="VTS1"
/locus_tag="YOR359W"
/db_xref="GeneID:854541"
mRNA <1011189..>1012760
/gene="VTS1"
/locus_tag="YOR359W"
/product="Vts1p"
/transcript_id="NM_001183779.3"
/db_xref="GeneID:854541"
CDS 1011189..1012760
/gene="VTS1"
/locus_tag="YOR359W"
/experiment="EXISTENCE:direct assay:GO:0000932 P-body
[PMID:18469165]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:12858164|PMID:16429156]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:20007605]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:11445562]"
/experiment="EXISTENCE:direct assay:GO:0051054 positive
regulation of DNA metabolic process [PMID:20007605]"
/experiment="EXISTENCE:direct assay:GO:0070336
flap-structured DNA binding [PMID:20007605]"
/experiment="EXISTENCE:mutant phenotype:GO:0000289
nuclear-transcribed mRNA poly(A) tail shortening
[PMID:18469165]"
/experiment="EXISTENCE:mutant phenotype:GO:0000956
nuclear-transcribed mRNA catabolic process
[PMID:12858164|PMID:16429156]"
/experiment="EXISTENCE:physical interaction:GO:0000289
nuclear-transcribed mRNA poly(A) tail shortening
[PMID:18469165]"
/experiment="EXISTENCE:physical interaction:GO:0051054
positive regulation of DNA metabolic process
[PMID:20007605]"
/note="Flap-structured DNA-binding and RNA-binding
protein; stimulates deadenylation-dependent mRNA
degradation mediated by the CCR4-NOT deadenylase complex;
member of the Smaug (Smg) family of post-transcriptional
regulators which bind RNA through a conserved sterile
alpha motif (SAM) domain that interacts with Smg
recognition element (SREs) containing transcripts;
stimulates Dna2p endonuclease activity; forms [SMAUG+]
prion"
/codon_start=1
/product="Vts1p"
/protein_id="NP_015004.3"
/db_xref="GeneID:854541"
/db_xref="SGD:S000005886"
/translation="MKHPYEEFPTGSKSPYNMSRGAHPGAVLLSPQSSAINKNNPGSN
SGNNQGNSSVTANVLSPQSHSMSLNDMLDQQSFMLDTAGTRAQPLQQQQQQQQQQQQA
SLPSLNIQTVSSTAAGSAIVSPMMQSPKALQSTLSSTSMYLDSFQRSPNNILGIPSQS
GSIPLPQSRQSQQQSQSQKNDPNMGTNFSQDINQLCSWISMLNSSQQNTVMDNILSIL
NDDVLKYTKLKIETLTNTPFISPPLPAIASPIPNRDDTQILNIDSVFSSSPITNDPEN
TDNLLYQNWSPQPHSIPISQPIYDNITDASQRSKSAEPHVNSSPNLIPVQKQFNNGNS
TKYKKLPSENPNYLSHSLSSSHSFFQPKKRSNMGNEYNSHHHHSLHHPLHNTTSYFSN
TSRPSGTDLNKSNQNVFNNTITHPNAGPTSATSTSTSSNGNTPLSSNSSMNPKSLTDP
KLLKNIPMWLKSLRLHKYSDALSGTPWIELIYLDDETLEKKGVLALGARRKLLKAFGI
VIDYKERDLIDRSAY"
gene complement(<1013241..>1014821)
/gene="PDE2"
/locus_tag="YOR360C"
/gene_synonym="SRA5"
/db_xref="GeneID:854542"
mRNA complement(<1013241..>1014821)
/gene="PDE2"
/locus_tag="YOR360C"
/gene_synonym="SRA5"
/product="3',5'-cyclic-nucleotide phosphodiesterase PDE2"
/transcript_id="NM_001183780.1"
/db_xref="GeneID:854542"
CDS complement(1013241..1014821)
/gene="PDE2"
/locus_tag="YOR360C"
/gene_synonym="SRA5"
/EC_number="3.1.4.53"
/experiment="EXISTENCE:direct assay:GO:0004115
3',5'-cyclic-AMP phosphodiesterase activity
[PMID:3025832]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0007189
adenylate cyclase-activating G protein-coupled receptor
signaling pathway [PMID:8391474]"
/note="High-affinity cyclic AMP phosphodiesterase;
component of the cAMP-dependent protein kinase signaling
system, protects the cell from extracellular cAMP,
contains readthrough motif surrounding termination codon"
/codon_start=1
/product="3',5'-cyclic-nucleotide phosphodiesterase PDE2"
/protein_id="NP_015005.1"
/db_xref="GeneID:854542"
/db_xref="SGD:S000005887"
/translation="MSTLFLIGIHEIEKSQTIVQNEHYFDRVIELQDLDSLMVALYKD
RVSPFPNVHNFETGVSIVLYDPSKFQLSVRQLDVLFKRFFPSFNISAIDHTREENLQR
LECVERENSICRNRITRINHWMYHHHDDTPDGINKNSYGTVNGNSVPTQACEANIYTL
LLHLNDSKAQHLRKASVPRLIRNIEFMSFLSDPIEKISQEGSHYWNILSTWDFCALSL
STQELIWCGFTLIKKLSKDAKVLIADNKLLLLLFTLESSYHQVNKFHNFRHAIDVMQA
TWRLCTYLLKDNPVQTLLLCMAAIGHDVGHPGTNNQLLCNCESEVAQNFKNVSILENF
HRELFQQLLSEHWPQLLSISKKKFDFISEAILATDMALHSQYEDRLMHENPMKQITLI
SLIIKAADISNVTRTLSISARWAYLITLEFNDCALLETFHKAHRPEQDCFGDSYKNVD
SPKEDLESIQNILVNVTDPDDIIKDHPHIPNGQIFFINTFAEVFFNALSQKFSGLKFL
SDNVKINKEYWMKHKKPQ"
gene complement(<1015361..>1017652)
/gene="PRT1"
/locus_tag="YOR361C"
/gene_synonym="CDC63; DNA26"
/db_xref="GeneID:854543"
mRNA complement(<1015361..>1017652)
/gene="PRT1"
/locus_tag="YOR361C"
/gene_synonym="CDC63; DNA26"
/product="translation initiation factor eIF3 core subunit
b"
/transcript_id="NM_001183781.3"
/db_xref="GeneID:854543"
CDS complement(1015361..1017652)
/gene="PRT1"
/locus_tag="YOR361C"
/gene_synonym="CDC63; DNA26"
/experiment="EXISTENCE:direct assay:GO:0002183 cytoplasmic
translational initiation [PMID:7798228]"
/experiment="EXISTENCE:direct assay:GO:0003743 translation
initiation factor activity [PMID:9671501|PMID:7876188]"
/experiment="EXISTENCE:direct assay:GO:0005852 eukaryotic
translation initiation factor 3 complex
[PMID:9660829|PMID:9671501]"
/experiment="EXISTENCE:direct assay:GO:0006413
translational initiation [PMID:9671501]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405|PMID:19470581]"
/experiment="EXISTENCE:direct assay:GO:0033290 eukaryotic
48S preinitiation complex [PMID:17242201]"
/experiment="EXISTENCE:direct assay:GO:0043614 multi-eIF
complex [PMID:15838098|PMID:11018020]"
/experiment="EXISTENCE:mutant phenotype:GO:0006413
translational initiation [PMID:3029094]"
/experiment="EXISTENCE:physical interaction:GO:0005852
eukaryotic translation initiation factor 3 complex
[PMID:9660829]"
/note="eIF3b subunit of the eukaryotic translation
initiation factor 3 (eIF3); subunit of the core complex of
eIF3; essential for translation; part of a subcomplex
(Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and
tRNA(i)Met to ribosomes; eIF3 is also involved in
programmed stop codon readthrough"
/codon_start=1
/product="translation initiation factor eIF3 core subunit
b"
/protein_id="NP_015006.3"
/db_xref="GeneID:854543"
/db_xref="SGD:S000005888"
/translation="MKNFLPRTLKNIYELYFNNISVHSIVSRNTQLKRSKIIQMTTET
FEDIKLEDIPVDDIDFSDLEEQYKVTEEFNFDQYIVVNGAPVIPSAKVPVLKKALTSL
FSKAGKVVNMEFPIDEATGKTKGFLFVECGSMNDAKKIIKSFHGKRLDLKHRLFLYTM
KDVERYNSDDFDTEFREPDMPTFVPSSSLKSWLMDDKVRDQFVLQDDVKTSVFWNSMF
NEEDSLVESRENWSTNYVRFSPKGTYLFSYHQQGVTAWGGPNFDRLRRFYHPDVRNSS
VSPNEKYLVTFSTEPIIVEEDNEFSPFTKKNEGHQLCIWDIASGLLMATFPVIKSPYL
KWPLVRWSYNDKYCARMVGDSLIVHDATKNFMPLEAKALKPSGIRDFSFAPEGVKLQP
FRNGDEPSVLLAYWTPETNNSACTATIAEVPRGRVLKTVNLVQVSNVTLHWQNQAEFL
CFNVERHTKSGKTQFSNLQICRLTERDIPVEKVELKDSVFEFGWEPHGNRFVTISVHE
VADMNYAIPANTIRFYAPETKEKTDVIKRWSLVKEIPKTFANTVSWSPAGRFVVVGAL
VGPNMRRSDLQFYDMDYPGEKNINDNNDVSASLKDVAHPTYSAATNITWDPSGRYVTA
WSSSLKHKVEHGYKIFNIAGNLVKEDIIAGFKNFAWRPRPASILSNAERKKVRKNLRE
WSAQFEEQDAMEADTAMRDLILHQRELLKQWTEYREKIGQEMEKSMNFKIFDVQPEDA
SDDFTTIEEIVEEVLEETKEKVE"
gene complement(<1017880..>1018746)
/gene="PRE10"
/locus_tag="YOR362C"
/db_xref="GeneID:854544"
mRNA complement(<1017880..>1018746)
/gene="PRE10"
/locus_tag="YOR362C"
/product="proteasome core particle subunit alpha 7"
/transcript_id="NM_001183782.1"
/db_xref="GeneID:854544"
CDS complement(1017880..1018746)
/gene="PRE10"
/locus_tag="YOR362C"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:21124907]"
/experiment="EXISTENCE:direct assay:GO:0010499 proteasomal
ubiquitin-independent protein catabolic process
[PMID:19162040]"
/experiment="EXISTENCE:direct assay:GO:0019773 proteasome
core complex, alpha-subunit complex [PMID:9087403]"
/experiment="EXISTENCE:direct assay:GO:0034515 proteasome
storage granule [PMID:18504300]"
/experiment="EXISTENCE:direct assay:GO:0043161
proteasome-mediated ubiquitin-dependent protein catabolic
process [PMID:19029916|PMID:11545745]"
/note="Alpha 7 subunit of the 20S proteasome; protein
abundance increases in response to DNA replication stress"
/codon_start=1
/product="proteasome core particle subunit alpha 7"
/protein_id="NP_015007.1"
/db_xref="GeneID:854544"
/db_xref="SGD:S000005889"
/translation="MTSIGTGYDLSNSVFSPDGRNFQVEYAVKAVENGTTSIGIKCND
GVVFAVEKLITSKLLVPQKNVKIQVVDRHIGCVYSGLIPDGRHLVNRGREEAASFKKL
YKTPIPIPAFADRLGQYVQAHTLYNSVRPFGVSTIFGGVDKNGAHLYMLEPSGSYWGY
KGAATGKGRQSAKAELEKLVDHHPEGLSAREAVKQAAKIIYLAHEDNKEKDFELEISW
CSLSETNGLHKFVKGDLLQEAIDFAQKEINGDDDEDEDDSDNVMSSDDENAPVATNAN
ATTDQEGDIHLE"
gene complement(<1020222..>1023212)
/gene="PIP2"
/locus_tag="YOR363C"
/gene_synonym="OAF2"
/db_xref="GeneID:854545"
mRNA complement(<1020222..>1023212)
/gene="PIP2"
/locus_tag="YOR363C"
/gene_synonym="OAF2"
/product="oleate-activated transcription factor PIP2"
/transcript_id="NM_001183783.3"
/db_xref="GeneID:854545"
CDS complement(1020222..1023212)
/gene="PIP2"
/locus_tag="YOR363C"
/gene_synonym="OAF2"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:9288897]"
/experiment="EXISTENCE:direct assay:GO:0001228 DNA-binding
transcription activator activity, RNA polymerase
II-specific [PMID:10428786]"
/experiment="EXISTENCE:direct assay:GO:0071400 cellular
response to oleic acid [PMID:9288897|PMID:10428786]"
/experiment="EXISTENCE:direct assay:GO:0089716 Pip2-Oaf1
complex [PMID:9288897]"
/experiment="EXISTENCE:genetic interaction:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:9288897]"
/experiment="EXISTENCE:mutant phenotype:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:9288897]"
/experiment="EXISTENCE:mutant phenotype:GO:0001228
DNA-binding transcription activator activity, RNA
polymerase II-specific [PMID:10428786|PMID:8972187]"
/experiment="EXISTENCE:mutant phenotype:GO:0032000
positive regulation of fatty acid beta-oxidation
[PMID:8972187|PMID:8670793]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:9288897|PMID:8972187|PMID:8670793]"
/experiment="EXISTENCE:mutant phenotype:GO:0071400
cellular response to oleic acid
[PMID:9288897|PMID:8972187|PMID:10428786]"
/experiment="EXISTENCE:physical interaction:GO:0089716
Pip2-Oaf1 complex [PMID:8972187]"
/note="Autoregulatory, oleate-activated transcription
factor; subunit of a heterodimeric complex with Oaf1p,
which binds to oleate-response elements (ORE) in the
promoter of genes involved in beta-oxidation of fatty
acids, peroxisome organization and biogenesis, activating
transcription in the presence of oleate; PIP2 has a
paralog, OAF1, that arose from the whole genome
duplication"
/codon_start=1
/product="oleate-activated transcription factor PIP2"
/protein_id="NP_015008.3"
/db_xref="GeneID:854545"
/db_xref="SGD:S000005890"
/translation="MYFTDESSPAMNRVGKKRNRLSFVCQACRKAKTKCDQEKPRCGR
CTKQNLFCIYDVARQAAPRNPNKDATIARLKKEIRYWRNKTVDLTQEKKDFYTALKRP
TEELAARRTCKSLQENSFPISLYKTHPRLIMTKVMKREINPLSEKYLIFQDTFLKTLI
ASVLLSCSRNSMIPALNADISRSRTQPCVKNNVVKMREVLLKNSKYESQRKSINEFTD
RLLQRKNPEEQIAVNKVISLLYSNRESSYLEDTCPTENDYSDLLKGYINEIEKTLPPK
AIIEQYLSHFFEHIFHLIPFASKEMLEESIHTTVQYNELGEVRLSMGTTLIRNKMENL
CILLLILRIAYISLTFIEDKIEDYSPYITKEMLEQYPIQSEVIFLAQQILASENWCAC
ANENTISCLLYIWCAFVFSPTEGDFLLEQPSDVIINLVILIGTSIGLHRDPSDFPALN
HPEASDKRLLNLRRIQWLSIISMATLESSLKGRLLVSPLSMIDLFIDVRDPNCVEIYK
KRVKKDLTGSESDEQLLEIHEIFFHRAQLALFLSDLNNITISYSGSVPMDTLETLRVK
ANELLKNKFQLRSVDINIYDEEKTFQKLTFNSILNSISLSGQILGKLMMLRASIALML
YFETLAMERSECLSFFYKYFFQCCADTISLIRFFFLYFNGSYEKVLSSLVCFITTKVI
QLAVPTTMFTLLVIIMRVELAKNMLLVKCNECNARGDISDLPEIKEKIKSLDTIKENF
ERLLLEVYLLASQNLRFKYFYIFKMLTLFDVFIQRLRKGQLFSGLFVKVDKDLTTKKI
ATMLELTLGINLDKSDHLIDRLKGKNLTVNFTLDQLYQIIKEFDRIKNIGVADPQNSL
NPSKPNMKDNTPTIELLLNSSVENESVPPYSSSNDPTNVGNASTYSLAHNISNQNNEE
NMPPSIGSSESNRAAPNLNFMPINNNYNNSGSNINNNDNVKLPSNFKNYYDPPMSSLD
ISMDVPDIFGSLDFFDYDLLFQND"
gene complement(<1023461..>1025572)
/locus_tag="YOR365C"
/db_xref="GeneID:854548"
mRNA complement(<1023461..>1025572)
/locus_tag="YOR365C"
/product="putative flavin adenine dinucleotide
transporter"
/transcript_id="NM_001183785.1"
/db_xref="GeneID:854548"
CDS complement(1023461..1025572)
/locus_tag="YOR365C"
/experiment="EXISTENCE:genetic interaction:GO:0030148
sphingolipid biosynthetic process [PMID:27462707]"
/note="Putative protein involved in sphingolipid
biosynthesis; not an essential gene; YOR365C has a
paralog, FLC2, that arose from the whole genome
duplication"
/codon_start=1
/product="putative flavin adenine dinucleotide
transporter"
/protein_id="NP_015010.1"
/db_xref="GeneID:854548"
/db_xref="SGD:S000005892"
/translation="MLPIKVFSLSRIFVLLAIFRQIHAISINSSTFQVSNSFTLLNNA
STLFPVPFDAYTKEYLRTSALLTCAKDSQFSASYFEAAFFPRNNTLFFNVEAQTIMSE
NITIKAELIAYGLNVYTKVFDLCGIQDNLLCPLKPGNIELMGSYYVEAAVASQIPSIT
YNIPDLDAYIVVTAYSTIDKEFTKPLACVQVMLSNGRTVQTEYLSWNLVILTISGIMF
SVVYSLQGYTVTSTRLASYSISLLLYFQNLAILAMISVSFLPPIVAAWTQNFQWSMGI
IRINFMQRLFDWYVVATSGSPTVVYRNKEVLSISVQKRSLNSKIISASSNLNGIESSQ
KNDLLYTSNLRNSNDYLSKILVLRGIKRVSYKAGIEISNFFLTGFSFFIIFICMIILA
FIIFKTFLKVLQKFKVKTIRYLHFHTHWSALLQGTLYRAVFIIYSEISLLALWEFTQT
DSAATLVEAIVVFILITVLLSSASVRIWRKMIKSKKIFGQPSYLLFSSSKFLNKFGFL
SSQFKSTKVWWLMATSAHMLIRSILVGSLQAHGKSQSIGIFLNEAIYLILLCWMQPYM
DKTTNFFNISIHSLNLVNAFFFLFFSNLFQQPIAVSSFMGLIFFFLNAAFSLYLFIFI
LICFTMAIYYKHPDTRYKPIDDQRRSFLKGEMDDDAVYESVPELHEMKKAVLECNGIQ
KPQINKIDLCKQNSNCEYLYM"
gene <1026007..>1026609
/gene="SCP1"
/locus_tag="YOR367W"
/db_xref="GeneID:854549"
mRNA <1026007..>1026609
/gene="SCP1"
/locus_tag="YOR367W"
/product="Scp1p"
/transcript_id="NM_001183787.3"
/db_xref="GeneID:854549"
CDS 1026007..1026609
/gene="SCP1"
/locus_tag="YOR367W"
/experiment="EXISTENCE:direct assay:GO:0007015 actin
filament organization [PMID:12857851]"
/experiment="EXISTENCE:direct assay:GO:0030479 actin
cortical patch [PMID:12857851]"
/experiment="EXISTENCE:direct assay:GO:0030674
protein-macromolecule adaptor activity [PMID:12857851]"
/experiment="EXISTENCE:direct assay:GO:0051015 actin
filament binding [PMID:12857851]"
/note="Component of yeast cortical actin cytoskeleton;
binds and cross links actin filaments; originally
identified by its homology to calponin (contains a
calponin-like repeat) but the Scp1p domain structure is
more similar to transgelin"
/codon_start=1
/product="Scp1p"
/protein_id="NP_015012.3"
/db_xref="GeneID:854549"
/db_xref="SGD:S000005894"
/translation="MSYDKKADVTSLDEDLRQLRESKFSPEAIQNIKIWVYKSVLKEI
APPGDLLECLKDGTVLCKLANILYEADTGEANHISWKSSKMPFVQMDQISQFLSFSRK
YGVPEDELFQTIDLFEKKDPAIVFQTLKSLSRYANKKHTDRFPVLGPQLSTKKPRPPV
KSKPKHLQDGTGWSTFEYGYMKGASQATEGVVLGQRRDIV"
gene <1026843..>1028048
/gene="RAD17"
/locus_tag="YOR368W"
/db_xref="GeneID:854550"
mRNA <1026843..>1028048
/gene="RAD17"
/locus_tag="YOR368W"
/product="Rad17p"
/transcript_id="NM_001183788.1"
/db_xref="GeneID:854550"
CDS 1026843..1028048
/gene="RAD17"
/locus_tag="YOR368W"
/experiment="EXISTENCE:direct assay:GO:0003690
double-stranded DNA binding [PMID:12604797]"
/experiment="EXISTENCE:direct assay:GO:0030896 checkpoint
clamp complex [PMID:9891048|PMID:12604797]"
/experiment="EXISTENCE:mutant phenotype:GO:0000077 DNA
damage checkpoint signaling [PMID:9564050]"
/experiment="EXISTENCE:mutant phenotype:GO:0006302
double-strand break repair [PMID:17624540]"
/experiment="EXISTENCE:mutant phenotype:GO:0007131
reciprocal meiotic recombination [PMID:10511543]"
/experiment="EXISTENCE:physical interaction:GO:0005634
nucleus [PMID:11356855]"
/note="Checkpoint protein; involved in the activation of
the DNA damage and meiotic pachytene checkpoints; with
Mec3p and Ddc1p, forms a clamp that is loaded onto partial
duplex DNA; homolog of human and S. pombe Rad1 and U.
maydis Rec1 proteins"
/codon_start=1
/product="Rad17p"
/protein_id="NP_015013.1"
/db_xref="GeneID:854550"
/db_xref="SGD:S000005895"
/translation="MRINSELANKFSASTVHLEHITTALSCLTPFGSKDDVLIFIDAD
GLSFVRENNHVIKIQLLLSRELFMSYSYRNETEDHMKLCVKINHILDSVSVMNRNSDD
IVECTLSYDGHGSPFVLIFEDSFISERVEYSTYLIKDFDTNGLELDRERISFEAIIKG
EALHSALKDLKEIGCKECYVYAKTEANDENVFALISKSQLGFSKIKLPSNRSILEKLQ
VFDGDSTTVIDGFAVIGFFDFTSFDKIRKSTKIASKVLFRMDVHGVLSVNILSQTDDV
IITDTTRPSNNRPGSIRQLQLPKDYPGIVIEVCMLEKESIDEAAQTEIELLMETNELG
NRNSFKKSTIRKRYGTDKGNETSNDNLLQLNGKKIKLPSEEENNKNRESEDEENHCKY
PTKDIPIFF"
gene complement(<1028194..>1028625)
/gene="RPS12"
/locus_tag="YOR369C"
/db_xref="GeneID:854551"
mRNA complement(<1028194..>1028625)
/gene="RPS12"
/locus_tag="YOR369C"
/product="40S ribosomal protein eS12 RPS12"
/transcript_id="NM_001183789.3"
/db_xref="GeneID:854551"
CDS complement(1028194..1028625)
/gene="RPS12"
/locus_tag="YOR369C"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:3533916]"
/experiment="EXISTENCE:direct assay:GO:0022627 cytosolic
small ribosomal subunit [PMID:3533916]"
/experiment="EXISTENCE:direct assay:GO:1990145 maintenance
of translational fidelity [PMID:32408794]"
/experiment="EXISTENCE:mutant phenotype:GO:0000028
ribosomal small subunit assembly [PMID:32408794]"
/experiment="EXISTENCE:mutant phenotype:GO:0030490
maturation of SSU-rRNA [PMID:32408794]"
/note="Protein component of the small (40S) ribosomal
subunit; homologous to mammalian ribosomal protein S12, no
bacterial homolog"
/codon_start=1
/product="40S ribosomal protein eS12 RPS12"
/protein_id="NP_015014.3"
/db_xref="GeneID:854551"
/db_xref="SGD:S000005896"
/translation="MSDVEEVVEVQEETVVEQTAEVTIEDALKVVLRTALVHDGLARG
LRESTKALTRGEALLVVLVSSVTEANIIKLVEGLANDPENKVPLIKVADAKQLGEWAG
LGKIDREGNARKVVGASVVVVKNWGAETDELSMIMEHFSQQ"
gene complement(<1029183..>1030994)
/gene="MRS6"
/locus_tag="YOR370C"
/gene_synonym="MSI4"
/db_xref="GeneID:854552"
mRNA complement(<1029183..>1030994)
/gene="MRS6"
/locus_tag="YOR370C"
/gene_synonym="MSI4"
/product="GTPase-activating protein MRS6"
/transcript_id="NM_001183790.1"
/db_xref="GeneID:854552"
CDS complement(1029183..1030994)
/gene="MRS6"
/locus_tag="YOR370C"
/gene_synonym="MSI4"
/experiment="EXISTENCE:direct assay:GO:0004663 Rab
geranylgeranyltransferase activity [PMID:8183917]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:9202009|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005968 Rab-protein
geranylgeranyltransferase complex
[PMID:8583924|PMID:8183917]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:9202009]"
/experiment="EXISTENCE:mutant phenotype:GO:0004663 Rab
geranylgeranyltransferase activity [PMID:8183917]"
/experiment="EXISTENCE:mutant phenotype:GO:0006612 protein
targeting to membrane [PMID:8183917]"
/experiment="EXISTENCE:mutant phenotype:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:8132658]"
/experiment="EXISTENCE:mutant phenotype:GO:0018344 protein
geranylgeranylation [PMID:12972569]"
/experiment="EXISTENCE:mutant phenotype:GO:0031267 small
GTPase binding [PMID:12972569]"
/note="Rab escort protein; type II
geranylgeranyltransferase complex (Bet2p-Bet4p) chaperone
that complexes with newly synthesized Rab GTPases, like
Ypt1p and Sec4p, leading to their prenylation and membrane
delivery; modulates the TOR pathway through interactions
with Sfp1p; alters the kinetics of MAPK pathway activation
and polarity reorganization during filamentous growth;
sequence similarity to mammalian choroideraemia gene;
relative distribution to the nucleus increases upon DNA
replication stress"
/codon_start=1
/product="GTPase-activating protein MRS6"
/protein_id="NP_015015.1"
/db_xref="GeneID:854552"
/db_xref="SGD:S000005897"
/translation="MLSPERRPSMAERRPSFFSFTQNPSPLVVPHLAGIEDPLPATTP
DKVDVLIAGTGMVESVLAAALAWQGSNVLHIDKNDYYGDTSATLTVDQIKRWVNEVNE
GSVSCYKNAKLYVSTLIGSGKYSSRDFGIDLSPKILFAKSDLLSILIKSRVHQYLEFQ
SLSNFHTYENDCFEKLTNTKQEIFTDQNLPLMTKRNLMKFIKFVLNWEAQTEIWQPYA
ERTMSDFLGEKFKLEKPQVFELIFSIGLCYDLNVKVPEALQRIRRYLTSFDVYGPFPA
LCSKYGGPGELSQGFCRSAAVGGATYKLNEKLVSFNPTTKVATFQDGSKVEVSEKVII
SPTQAPKDSKHVPQQQYQVHRLTCIVENPCTEWFNEGESAAMVVFPPGSLKSGNKEVV
QAFILGAGSEICPEGTIVWYLSTTEQGPRAEMDIDAALEAMEMALLRESSSGLENDEE
IVQLTGNGHTIVNSVKLGQSFKEYVPRERLQFLFKLYYTQYTSTPPFGVVNSSFFDVN
QDLEKKYIPGASDNGVIYTTMPSAEISYDEVVTAAKVLYEKIVGSDDDFFDLDFEDED
EIQASGVANAEQFENAIDDDDDVNMEGSGEFVGEMEI"
gene complement(<1031489..>1034182)
/gene="GPB1"
/locus_tag="YOR371C"
/gene_synonym="KRH2"
/db_xref="GeneID:854553"
mRNA complement(<1031489..>1034182)
/gene="GPB1"
/locus_tag="YOR371C"
/gene_synonym="KRH2"
/product="Gpb1p"
/transcript_id="NM_001183791.3"
/db_xref="GeneID:854553"
CDS complement(1031489..1034182)
/gene="GPB1"
/locus_tag="YOR371C"
/gene_synonym="KRH2"
/experiment="EXISTENCE:direct assay:GO:0000209 protein
polyubiquitination [PMID:20160012]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16030250]"
/experiment="EXISTENCE:direct assay:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:20160012]"
/experiment="EXISTENCE:genetic interaction:GO:0001403
invasive growth in response to glucose limitation
[PMID:12150916]"
/experiment="EXISTENCE:genetic interaction:GO:0004862
cAMP-dependent protein kinase inhibitor activity
[PMID:16278446|PMID:16924114]"
/experiment="EXISTENCE:genetic interaction:GO:0007124
pseudohyphal growth [PMID:12150916]"
/experiment="EXISTENCE:genetic interaction:GO:0042326
negative regulation of phosphorylation [PMID:16278446]"
/experiment="EXISTENCE:genetic interaction:GO:0046580
negative regulation of Ras protein signal transduction
[PMID:16793550]"
/experiment="EXISTENCE:mutant phenotype:GO:0001403
invasive growth in response to glucose limitation
[PMID:12150916]"
/experiment="EXISTENCE:mutant phenotype:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:20160012]"
/experiment="EXISTENCE:mutant phenotype:GO:0007124
pseudohyphal growth [PMID:12150916]"
/experiment="EXISTENCE:mutant phenotype:GO:0010255 glucose
mediated signaling pathway [PMID:12150916]"
/experiment="EXISTENCE:mutant phenotype:GO:0046579
positive regulation of Ras protein signal transduction
[PMID:20160012]"
/experiment="EXISTENCE:physical interaction:GO:0032794
GTPase activating protein binding [PMID:16793550]"
/note="Multistep regulator of cAMP-PKA signaling; inhibits
PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP
dependency; promotes ubiquitin-dependent proteolysis of
Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a
paralog, GPB2, that arose from the whole genome
duplication"
/codon_start=1
/product="Gpb1p"
/protein_id="NP_015016.3"
/db_xref="GeneID:854553"
/db_xref="SGD:S000005898"
/translation="MPQASTFGSHSLEAHPLHIQPAVHIKLSKEERSHYREQYDSLKY
ISNYVSVFDQALSDNIDSRIRKENEALLKKYYESRKPFTFTSFRQGSVISSSDSSTGF
TERTKTYCFLNDFVSNCVNEVDPYTLKMTVRNRNTALNMENLDDERKSKDDIYDFEDN
TDDECNAKCHGAFHYSSERLEILRSRSTISYFKYYKKLLTVDLRDSDVLKRHNLWMPM
ITRRFRFLLVSSSKPEDVRLTTPIPTFSESDLDIFKNKTCPLFINGTDCVPRSYDTFS
GSSVIASIFSEYKLPSLSYHCSVELNDQLFIVGGLMACHRYDEEAPDLKDFYVDGIKN
LPPPLIPELINNPSMIPNPHLYCFSLTSSRLTRPDISGYIPPPLVCTQGCKLTERHIF
LYGGFEIKSETQVDDKGRYFIRKRAFLNNTGYILDTVTFNFSKIELVAPPYQFAIYNN
FSPRFGHMQASISNSNNNVSNENTTTSAKGRRSISPYRQGNGDHKIDDLVGSPGSTDY
LEDDAIPPVTNPRSTDSLSSKHCSTATHICSSVNTILIFGGYSQTGDDKYEAMNDMWK
INIPVVSRGKRNYYKFADTVTATKIPIIDDPELWPSRRAFSACCVPDYFTKDVEPIET
RLLRNLKNDFSIDLEIRPGNKPSQPLFPNIPHSRKEKKSGRDSMHISNSNNSTSEDTS
SKSTRNTTSSPPTSPKHTPPLNPSKKCASIGRTIAFHGGSDGYDVCSDMWWFDFDSET
WTKIDLYAKTQEESDGLVPINLCMVGHSMTTVGHKVVLIGGLRQGDVDRIYRDETLPE
EVISGVPLGSGVINVVDLNTQCLQGCKLIRNDGDTKESVIMDPHVGTPHQVLAVAGTI
ELVKGTMTLIGGVVAGREDISSLYLRGAVLQFILPSMNLAN"
gene complement(<1034807..>1036471)
/gene="NDD1"
/locus_tag="YOR372C"
/db_xref="GeneID:854554"
mRNA complement(<1034807..>1036471)
/gene="NDD1"
/locus_tag="YOR372C"
/product="Ndd1p"
/transcript_id="NM_001183792.1"
/db_xref="GeneID:854554"
CDS complement(1034807..1036471)
/gene="NDD1"
/locus_tag="YOR372C"
/experiment="EXISTENCE:direct assay:GO:0003713
transcription coactivator activity [PMID:10207056]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10207056]"
/experiment="EXISTENCE:mutant phenotype:GO:0000086 G2/M
transition of mitotic cell cycle [PMID:10207056]"
/experiment="EXISTENCE:mutant phenotype:GO:0003713
transcription coactivator activity [PMID:10207056]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:10207056]"
/note="Transcriptional activator essential for nuclear
division; localized to the nucleus; essential component of
the mechanism that activates the expression of a set of
late-S-phase-specific genes; turnover is tightly regulated
during cell cycle and in response to DNA damage"
/codon_start=1
/product="Ndd1p"
/protein_id="NP_015017.1"
/db_xref="GeneID:854554"
/db_xref="SGD:S000005899"
/translation="MDRDISYQQNYTSTGATATSSRQPSTDNNADTNFLKVMSEFKYN
FNSPLPTTTQFPTPYSSNQYQQTQDHFANTDAHNSSSNESSLVENSILPHHQQIQQQQ
QQQQQQQQQQQALGSLVPPAVTRTDTSETLDDINVQPSSVLQFGNSLPSEFLVASPEQ
FKEFLLDSPSTNFNFFHKTPAKTPLRFVTDSNGAQQSTTENPGQQQNVFSNVDLNNLL
KSNGKTPSSSCTGAFSRTPLSKIDMNLMFNQPLPTSPSKRFSSLSLTPYGRKILNDVG
TPYAKALISSNSALVDFQKARKDITTNATSIGLENANNILQRTPLRSNNKKLFIKTPQ
DTINSTSTLTKDNENKQDIYGSSPTTIQLNSSITKSISKLDNSRIPLLASRSDNILDS
NVDDQLFDLGLTRLPLSPTPNCNSLHSTTTGTSALQIPELPKMGSFRSDTGINPISSS
NTVSFKSKSGNNNSKGRIKKNGKKPSKFQIIVANIDQFNQDTSSSSLSSSLNASSSAG
NSNSNVTKKRASKLKRSQSLLSDSGSKSQARKSCNSKSNGNLFNSQ"
gene <1036834..>1039389
/gene="NUD1"
/locus_tag="YOR373W"
/gene_synonym="CDC18"
/db_xref="GeneID:854555"
mRNA <1036834..>1039389
/gene="NUD1"
/locus_tag="YOR373W"
/gene_synonym="CDC18"
/product="Nud1p"
/transcript_id="NM_001183793.3"
/db_xref="GeneID:854555"
CDS 1036834..1039389
/gene="NUD1"
/locus_tag="YOR373W"
/gene_synonym="CDC18"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:36164978]"
/experiment="EXISTENCE:direct assay:GO:0061499 outer
plaque of mitotic spindle pole body [PMID:9585415]"
/experiment="EXISTENCE:genetic interaction:GO:0031536
positive regulation of exit from mitosis [PMID:11101520]"
/experiment="EXISTENCE:genetic interaction:GO:0051293
establishment of spindle localization [PMID:22385961]"
/experiment="EXISTENCE:mutant phenotype:GO:0000073 initial
mitotic spindle pole body separation [PMID:22385961]"
/experiment="EXISTENCE:mutant phenotype:GO:0030953 astral
microtubule organization [PMID:11101520]"
/experiment="EXISTENCE:mutant phenotype:GO:0031536
positive regulation of exit from mitosis
[PMID:11101520|PMID:23579499]"
/experiment="EXISTENCE:mutant phenotype:GO:0035591
signaling adaptor activity [PMID:23579499]"
/experiment="EXISTENCE:mutant phenotype:GO:0045132 meiotic
chromosome segregation [PMID:16888627]"
/experiment="EXISTENCE:mutant phenotype:GO:0051293
establishment of spindle localization [PMID:22385961]"
/experiment="EXISTENCE:physical interaction:GO:0035591
signaling adaptor activity [PMID:23579499]"
/note="Mitotic exit network (MEN) scaffold protein; core
component of the SPB outer plaque that functions in astral
microtubule organization via Spc72p; scaffold for
recruitment of MEN components, such as Cdc15p, Tem1p and
the Bfa1p-Bub2p checkpoint complex; phosphorylation by
Cdc15p creates a Dbf2p-Mob1p phospho-docking site enabling
Cdc15p-dependent complex activation and mitotic exit; acts
through the MEN to specify Kar9p-mediated asymmetric SPB
inheritance; homologous to mammalian centriolin"
/codon_start=1
/product="Nud1p"
/protein_id="NP_015018.3"
/db_xref="GeneID:854555"
/db_xref="SGD:S000005900"
/translation="MDMDTQEAELSSQLENLTINSPRKLRSNAHSNSGKVFKEYESNH
DFQDSNFTSQVVEPAISDSVKKPPTMTVLNNYSTVHQKVPSGFSGTTATSHQEAQWKQ
YFPGIGSGGGTNFGGAVGTANKVPESDLIVSDLVKDLSGVLETNTFKRHLDMKNKTTT
MQTHENHDTISISHSKDFFNAEKVSSSFSDDSDSGPAAEAHDVFDGILQKQKSNYLVG
SYPSNSNNKNNNNNNNNNNNNSININNKDNARTKEEDEEDTSNSFEFSSSSSMSSSQT
QSGRKSKVLKKPPLNTISPGQLGYQFNHTHGAWDPPLNQGLDVSSSHSLDNTSSNQSQ
FATMVPTGDNHTNGKAPSILDKKAYELTSTKPGDVGYRQKKIQEEENLANSDDTPLDT
PKFNDLFTKNGTRAKVKGQMRTSRSISNSNLLEAHKKLKTFPAERVEDITSISEVNTS
FNETEKQLISILTSKLSGSPSYDSDWEKILKVDLSRGKLKNMFGMQRLLPNVLVLNLS
DNEMNTLEGIPSNVVQLFCSNNKITSAHCSLAGFHDLECLDLSYNLLNTSLKFLSLCH
HLQEVNLSYNSIQSLEGIGSSRMKKLNLSNNEINGIIDFEQLILTNNSVVGGWLTVEV
LDLSNNNIIGVRNINCLPRLKVLNLNGNPLVSIVESSKMENGTLRALSIKNTGGALSK
LQNYKLDDQFTFPYQNLKILKLDGFAQLSKWQKWPATLQILEINGGLASSLPRFSSLK
STNLYSLTIANVRDFTHLPVDLSKELPFLQELHLPGNNLQNAHKLTKTLPRQSVKFLD
LRNNPITTPRHDRASTSLHYRQLLQLAGLCQQQCPALATLWLDDTPAPTATNL"
gene <1039840..>1041399
/gene="ALD4"
/locus_tag="YOR374W"
/gene_synonym="ALD7"
/db_xref="GeneID:854556"
mRNA <1039840..>1041399
/gene="ALD4"
/locus_tag="YOR374W"
/gene_synonym="ALD7"
/product="aldehyde dehydrogenase (NADP(+)) ALD4"
/transcript_id="NM_001183794.1"
/db_xref="GeneID:854556"
CDS 1039840..1041399
/gene="ALD4"
/locus_tag="YOR374W"
/gene_synonym="ALD7"
/EC_number="1.2.1.4"
/experiment="EXISTENCE:direct assay:GO:0004029 aldehyde
dehydrogenase (NAD+) activity [PMID:9473035]"
/experiment="EXISTENCE:direct assay:GO:0004030 aldehyde
dehydrogenase [NAD(P)+] activity [PMID:9473035]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:16823961|PMID:14576278|PMID:24769239|PMID:11502169]"
/experiment="EXISTENCE:direct assay:GO:0042645
mitochondrial nucleoid [PMID:10869431]"
/experiment="EXISTENCE:genetic interaction:GO:0006740
NADPH regeneration [PMID:19158096]"
/experiment="EXISTENCE:genetic interaction:GO:0019413
acetate biosynthetic process [PMID:10919763]"
/experiment="EXISTENCE:mutant phenotype:GO:0004029
aldehyde dehydrogenase (NAD+) activity [PMID:9675847]"
/experiment="EXISTENCE:mutant phenotype:GO:0006067 ethanol
metabolic process [PMID:9675847]"
/experiment="EXISTENCE:mutant phenotype:GO:0006629 lipid
metabolic process [PMID:39462103]"
/note="Mitochondrial aldehyde dehydrogenase; K+ dependent
enzyme that can use either NADP+ or NAD+ as cofactor;
required for growth on ethanol and for conversion of
acetaldehyde to acetate; can substitute for cytosolic
NADP-dependent aldehyde dehydrogenase when directed to the
cytosol; influences lipid metabolism; polymerizes into
filaments in meiotic cells; phosphorylated; expression is
glucose repressed; human homolog ALDH2 can complement a
yeast ald4 mutant"
/codon_start=1
/product="aldehyde dehydrogenase (NADP(+)) ALD4"
/protein_id="NP_015019.1"
/db_xref="GeneID:854556"
/db_xref="SGD:S000005901"
/translation="MFSRSTLCLKTSASSIGRLQLRYFSHLPMTVPIKLPNGLEYEQP
TGLFINNKFVPSKQNKTFEVINPSTEEEICHIYEGREDDVEEAVQAADRAFSNGSWNG
IDPIDRGKALYRLAELIEQDKDVIASIETLDNGKAISSSRGDVDLVINYLKSSAGFAD
KIDGRMIDTGRTHFSYTKRQPLGVCGQIIPWNFPLLMWAWKIAPALVTGNTVVLKTAE
STPLSALYVSKYIPQAGIPPGVINIVSGFGKIVGEAITNHPKIKKVAFTGSTATGRHI
YQSAAAGLKKVTLELGGKSPNIVFADAELKKAVQNIILGIYYNSGEVCCAGSRVYVEE
SIYDKFIEEFKAASESIKVGDPFDESTFQGAQTSQMQLNKILKYVDIGKNEGATLITG
GERLGSKGYFIKPTVFGDVKEDMRIVKEEIFGPVVTVTKFKSADEVINMANDSEYGLA
AGIHTSNINTALKVADRVNAGTVWINTYNDFHHAVPFGGFNASGLGREMSVDALQNYL
QVKAVRAKLDE"
gene complement(<1041678..>1043042)
/gene="GDH1"
/locus_tag="YOR375C"
/gene_synonym="DHE4; URE1"
/db_xref="GeneID:854557"
mRNA complement(<1041678..>1043042)
/gene="GDH1"
/locus_tag="YOR375C"
/gene_synonym="DHE4; URE1"
/product="glutamate dehydrogenase (NADP(+)) GDH1"
/transcript_id="NM_001183795.3"
/db_xref="GeneID:854557"
CDS complement(1041678..1043042)
/gene="GDH1"
/locus_tag="YOR375C"
/gene_synonym="DHE4; URE1"
/EC_number="1.4.1.4"
/experiment="EXISTENCE:direct assay:GO:0004354 glutamate
dehydrogenase (NADP+) activity
[PMID:11562373|PMID:2932370|PMID:2989290]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:4126]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:mutant phenotype:GO:0004354
glutamate dehydrogenase (NADP+) activity
[PMID:2932370|PMID:2989290]"
/experiment="EXISTENCE:mutant phenotype:GO:0006537
glutamate biosynthetic process
[PMID:2989290|PMID:2932370]"
/note="NADP(+)-dependent glutamate dehydrogenase;
synthesizes glutamate from ammonia and
alpha-ketoglutarate; rate of alpha-ketoglutarate
utilization differs from Gdh3p; expression regulated by
nitrogen and carbon sources; GDH1 has a paralog, GDH3,
that arose from the whole genome duplication"
/codon_start=1
/product="glutamate dehydrogenase (NADP(+)) GDH1"
/protein_id="NP_015020.3"
/db_xref="GeneID:854557"
/db_xref="SGD:S000005902"
/translation="MSEPEFQQAYEEVVSSLEDSTLFEQHPEYRKVLPIVSVPERIIQ
FRVTWENDKGEQEVAQGYRVQYNSAKGPYKGGLRFHPSVNLSILKFLGFEQIFKNSLT
GLDMGGGKGGLCVDLKGRSNNEIRRICYAFMRELSRHIGQDTDVPAGDIGVGGREIGY
LFGAYRSYKNSWEGVLTGKGLNWGGSLIRPEATGYGLVYYTQAMIDYATNGKESFEGK
RVTISGSGNVAQYAALKVIELGGTVVSLSDSKGCIISETGITSEQVADISSAKVNFKS
LEQIVNEYSTFSENKVQYIAGARPWTHVQKVDIALPCATQNEVSGEEAKALVAQGVKF
IAEGSNMGSTPEAIAVFETARSTATGPSEAVWYGPPKAANLGGVAVSGLEMAQNSQRI
TWTSERVDQELKRIMINCFNECIDYAKKYTKDGKVLPSLVKGANIASFIKVSDAMFDQ
GDVF"
gene <1043191..>1043559
/locus_tag="YOR376W"
/db_xref="GeneID:854558"
mRNA <1043191..>1043559
/locus_tag="YOR376W"
/product="uncharacterized protein"
/transcript_id="NM_001348882.1"
/db_xref="GeneID:854558"
CDS 1043191..1043559
/locus_tag="YOR376W"
/note="hypothetical protein; conserved among S. cerevisiae
strains; YOR376W is not an essential gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335820.1"
/db_xref="GeneID:854558"
/db_xref="SGD:S000005903"
/translation="MKNRKFSNLLLLRLRILCFNKKPAFAATSYAFFFRNFSVLIFIM
VPDEKENGAAADNSFSLLIGRGVVLFLFYCPTALKMHGPVPAHWFCDKNIEAIQSDGQ
IRLLRSGPFPWSHGTCIRGA"
gene <1045196..>1045351
/locus_tag="YOR376W-A"
/db_xref="GeneID:1466488"
mRNA <1045196..>1045351
/locus_tag="YOR376W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184578.1"
/db_xref="GeneID:1466488"
CDS 1045196..1045351
/locus_tag="YOR376W-A"
/note="hypothetical protein; identified by fungal homology
and RT-PCR"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878176.1"
/db_xref="GeneID:1466488"
/db_xref="SGD:S000028586"
/translation="MTFHLGWTILWYNQAYLEVWATVFQDEMHKYSLHPQSRDAKTKF
CCCIPFK"
gene <1046226..>1047803
/gene="ATF1"
/locus_tag="YOR377W"
/db_xref="GeneID:854559"
mRNA <1046226..>1047803
/gene="ATF1"
/locus_tag="YOR377W"
/product="alcohol O-acetyltransferase"
/transcript_id="NM_001183797.3"
/db_xref="GeneID:854559"
CDS 1046226..1047803
/gene="ATF1"
/locus_tag="YOR377W"
/EC_number="2.3.1.84"
/EC_number="3.1.2.20"
/experiment="EXISTENCE:direct assay:GO:0004026 alcohol
O-acetyltransferase activity [PMID:8085822]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005811 lipid
droplet [PMID:15042596]"
/experiment="EXISTENCE:genetic interaction:GO:1901089
acetate ester metabolic process involved in fermentation
[PMID:12957907]"
/experiment="EXISTENCE:mutant phenotype:GO:1901089 acetate
ester metabolic process involved in fermentation
[PMID:23200656|PMID:12957907]"
/note="Alcohol acetyltransferase; responsible for the
major part of volatile acetate ester production during
fermentation; main enzyme involved in terpenyl acetate
synthesis; potential roles in lipid and sterol metabolism"
/codon_start=1
/product="alcohol O-acetyltransferase"
/protein_id="NP_015022.3"
/db_xref="GeneID:854559"
/db_xref="SGD:S000005904"
/translation="MNEIDEKNQAPVQQECLKEMIQNGHARRMGSVEDLYVALNRQNL
YRNFCTYGELSDYCTRDQLTLALREICLKNPTLLHIVLPTRWPNHENYYRSSEYYSRP
HPVHDYISVLQELKLSGVVLNEQPEYSAVMKQILEEFKNSKGSYTAKIFKLTTTLTIP
YFGPTGPSWRLICLPEEHTEKWKKFIFVSNHCMSDGRSSIHFFHDLRDELNNIKTPPK
KLDYIFKYEEDYQLLRKLPEPIEKVIDFRPPYLFIPKSLLSGFIYNHLRFSSKGVCMR
MDDVEKTDDVVTEIINISPTEFQAIKANIKSNIQGKCTITPFLHVCWFVSLHKWGKFF
KPLNFEWLTDIFIPADCRSQLPDDDEMRQMYRYGANVGFIDFTPWISEFDMNDNKENF
WPLIEHYHEVISEALRNKKHLHGLGFNIQGFVQKYVNIDKVMCDRAIGKRRGGTLLSN
VGLFNQLEEPDAKYSICDLAFGQFQGSWHQAFSLGVCSTNVKGMNIVVASTKNVVGSQ
ESLEELCSIYKALLLGP"
gene <1049511..>1051058
/gene="AMF1"
/locus_tag="YOR378W"
/db_xref="GeneID:854560"
mRNA <1049511..>1051058
/gene="AMF1"
/locus_tag="YOR378W"
/product="Amf1p"
/transcript_id="NM_001183798.1"
/db_xref="GeneID:854560"
CDS 1049511..1051058
/gene="AMF1"
/locus_tag="YOR378W"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:24707045]"
/experiment="EXISTENCE:direct assay:GO:0008519 ammonium
channel activity [PMID:24707045]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0072488
ammonium transmembrane transport [PMID:24707045]"
/note="Low affinity NH4+ transporter; member of the DHA2
family of drug:H+ anti porters; putative paralog of ATR1;
but not required for boron tolerance; non-essential gene"
/codon_start=1
/product="Amf1p"
/protein_id="NP_015023.1"
/db_xref="GeneID:854560"
/db_xref="SGD:S000005905"
/translation="MSTSSSVTQKNLDTNAEALKKEDKVLSEFDIQDERPKSLLWESA
FVGVLCSAQLMTQAGLGQSLAPLHIIGNSFGTTNAGQLSWFASAYSLTVGTFILIAGR
LGDIFGHKKFFVLGFFWYALWSLLAGFSVYSNQIFFDCCRAFQGMGPAFLLPNAIAIL
GRTYKPGRRKNMVFSLFGASAPGGFFLGAVFSSMLGQLAWWPWAYWIMGIACFVLAVA
GYFVIPHTPMPSRDASSFKLLERIDFAGSVTGVVGLILFNFAWNQGPVVGWQTPYTYA
LLIVGTFFLVIFAYIESRAAFPLLPFAALSSDTAFVLSCIAAGWASFGIWIFYTWQFM
EDSRGQTPLLSSAQFSPVAISGFCAAVTTGFLLSHTPPSTVMLFAMTAFTVGTILIAT
APVHQTYWAQTFVSIIVMPWGMDMSFPAATIMLSDSMPHEHQGLAASLVNTVVNYSIS
IGLGIAGTIESRVNDGGAKPLKGYRCSWYMGIGLSGLGIFVAATYAWSTFMKSKKRIS
EKQHFIE"
gene <1051290..>1052930
/gene="RDR1"
/locus_tag="YOR380W"
/db_xref="GeneID:854562"
mRNA <1051290..>1052930
/gene="RDR1"
/locus_tag="YOR380W"
/product="Rdr1p"
/transcript_id="NM_001183800.3"
/db_xref="GeneID:854562"
CDS 1051290..1052930
/gene="RDR1"
/locus_tag="YOR380W"
/experiment="EXISTENCE:curator inference:GO:0005634
nucleus [PMID:11882665]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:19111667]"
/experiment="EXISTENCE:mutant phenotype:GO:0003700
DNA-binding transcription factor activity [PMID:11882665]"
/experiment="EXISTENCE:mutant phenotype:GO:0009410
response to xenobiotic stimulus [PMID:11882665]"
/note="Transcriptional repressor involved in regulating
multidrug resistance; negatively regulates expression of
the PDR5 gene; member of the Gal4p family of zinc cluster
proteins"
/codon_start=1
/product="Rdr1p"
/protein_id="NP_015025.3"
/db_xref="GeneID:854562"
/db_xref="SGD:S000005907"
/translation="MASPGSTALPHKRQRVRKACVPCRERKRKCNGKSPCEMCVAYGY
VCHYIDGRVPSASPQVQQVGETSPDTESRPFVLPGIHRNEQPQPINTQNVTSQNIVDP
TKSRYTIQHSAVAFPRCLGLELRSTNPPRLHSFAWHCGIRPEENPNSHVLLSDLVTKE
EYYRISKVYFSVVHPIFDVVNPEQLAKNVEKYWDGDVKTLEYGAVIAGVIALGSFFMG
SLGHPREMDIVQYAKGILDDPTFSRIPTVEQVSAWVLRTIYLRATSRPHVAWLASCVT
IHLSEAIGLHHEIDREDIAISNNVPPKRTTVVSEHTRRLFWCAWSINTILSYDYGRSS
VTLNRITCKPVKETDGNFTAHLVALAHLIPQDSVNANAAQLLQALAAVHESPNAHPFL
SLTKGDICLSLYRRLRLLNHILDKNVVLQIIDIGNTALSAAYALVKLDQAWWNVLSTS
FQYVCVLLAIDTPESLSHVATAMKTLDNITQILGTRIAFEAQKTAKLLLEDSMKKKRQ
EIQQLEQATHQRSNLETTHLLDIDWDALLDPSDTLNFM"
gene <1055545..>1057680
/gene="FRE3"
/locus_tag="YOR381W"
/db_xref="GeneID:854563"
mRNA <1055545..>1057680
/gene="FRE3"
/locus_tag="YOR381W"
/product="ferric-chelate reductase"
/transcript_id="NM_001183801.1"
/db_xref="GeneID:854563"
CDS 1055545..1057680
/gene="FRE3"
/locus_tag="YOR381W"
/EC_number="1.16.1.9"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:11120744]"
/experiment="EXISTENCE:genetic interaction:GO:0000293
ferric-chelate reductase activity [PMID:11120744]"
/experiment="EXISTENCE:mutant phenotype:GO:0006879
intracellular iron ion homeostasis
[PMID:10341420|PMID:9726978]"
/experiment="EXISTENCE:mutant phenotype:GO:0015891
siderophore transport [PMID:9726978]"
/note="Ferric reductase; reduces siderophore-bound iron
prior to uptake by transporters; expression induced by low
iron levels"
/codon_start=1
/product="ferric-chelate reductase"
/protein_id="NP_015026.1"
/db_xref="GeneID:854563"
/db_xref="SGD:S000005908"
/translation="MYWVLLCGSILLCCLSGASASPAKTKMYGKLPLVLTDACMGVLG
EVTWEYSSDDLYSSPACTYEPALQSMLYCIYESLNEKGYSNRTFEKTFAAIKEDCAYY
TDNLQNMTNADFYNMLNNGTTYIIQYSEGSANLTYPIEMDAQVRENYYYSYHGFYANY
DIGHTYGGIICAYFVGVMILASILHYLSYTPFKTALFKQRLVRYVRRYLTIPTIWGKH
ASSFSYLKIFTGFLPTRSEGVIILGYLVLHTVFLAYGYQYDPYNLIFDSRREQIARYV
ADRSGVLAFAHFPLIALFAGRNNFLEFISGVKYTSFIMFHKWLGRMMFLDAVIHGAAY
TSYSVFYKDWAASKEETYWQFGVAALCIVGVMVFFSLAMFRKFFYEAFLFLHIVLGAL
FFYTCWEHVVELSGIEWIYAAIAIWTIDRLIRIVRVSYFGFPKASLQLVGDDIIRVTV
KRPVRLWKAKPGQYVFVSFLHHLYFWQSHPFTVLDSIIKDGELTIILKEKKGVTKLVK
KYVCCNGGKASMRLAIEGPYGSSSPVNNYDNVLLLTGGTGLPGPIAHAIKLGKTSAAT
GKQFIKLVIAVRGFNVLEAYKPELMCLEDLNVQLHIYNTMEVPALTPNDSLEISQQDE
KADGKGVVMATTLEQSPNPVEFDGTVFHHGRPNVEKLLHEVGDLNGSLAVVCCGPPVF
VDEVRDQTANLVLEKPAKAIEYFEEYQSW"
gene <1058423..>1058590
/locus_tag="YOR381W-A"
/db_xref="GeneID:1466489"
mRNA <1058423..>1058590
/locus_tag="YOR381W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184579.3"
/db_xref="GeneID:1466489"
CDS 1058423..1058590
/locus_tag="YOR381W-A"
/note="hypothetical protein; identified by fungal homology
and RT-PCR"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878177.3"
/db_xref="GeneID:1466489"
/db_xref="SGD:S000028587"
/translation="MFNVALPSQDKKSEFYKQKRLSLRTNILDEFLRVAKTQDNRQNN
FVSQYRNDEIY"
gene <1059531..>1059992
/gene="FIT2"
/locus_tag="YOR382W"
/db_xref="GeneID:854564"
mRNA <1059531..>1059992
/gene="FIT2"
/locus_tag="YOR382W"
/product="Fit2p"
/transcript_id="NM_001183802.1"
/db_xref="GeneID:854564"
CDS 1059531..1059992
/gene="FIT2"
/locus_tag="YOR382W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:10383953]"
/experiment="EXISTENCE:direct assay:GO:0015891 siderophore
transport [PMID:12196168]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="Mannoprotein that is incorporated into the cell
wall; incorporated via a glycosylphosphatidylinositol
(GPI) anchor; involved in the retention of
siderophore-iron in the cell wall"
/codon_start=1
/product="Fit2p"
/protein_id="NP_015027.1"
/db_xref="GeneID:854564"
/db_xref="SGD:S000005909"
/translation="MKFSTIFGATTVMTAVSAAAVSSVMTTKTITATNGNNVYTKVVT
DTADPIISYSTTRTVVVSNSDATYTKVVTEGPDTTSEKSTTKTLTLTNGSGSSTNLYT
KTVTQAVESSTSSSSSSSSSSSSASSSGAAPAAFQGASVGALALGLISYLL"
gene complement(<1060441..>1061055)
/gene="FIT3"
/locus_tag="YOR383C"
/db_xref="GeneID:854565"
mRNA complement(<1060441..>1061055)
/gene="FIT3"
/locus_tag="YOR383C"
/product="Fit3p"
/transcript_id="NM_001183803.3"
/db_xref="GeneID:854565"
CDS complement(1060441..1061055)
/gene="FIT3"
/locus_tag="YOR383C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:10383953]"
/experiment="EXISTENCE:direct assay:GO:0015891 siderophore
transport [PMID:12196168]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="Mannoprotein that is incorporated into the cell
wall; incorporated via a glycosylphosphatidylinositol
(GPI) anchor; involved in the retention of
siderophore-iron in the cell wall"
/codon_start=1
/product="Fit3p"
/protein_id="NP_015028.3"
/db_xref="GeneID:854565"
/db_xref="SGD:S000005910"
/translation="MKFSSALVLSAVAATALAESITTTITATKNGHVYTKTVTQDATF
VWGGEDSYASSTSAAESSAAETSAAETSAAATTSAAATTSAAETSSAAETSSADEGSG
SSITTTITATKNGHVYTKTVTQDATFVWTGEGSSNTWSPSSTSTSSEAATSSASTTAT
TTAETSSSATSSSTAELSSYTGAADAITAGTGLMGAALAAVMLL"
gene <1061564..>1063648
/gene="FRE5"
/locus_tag="YOR384W"
/db_xref="GeneID:854566"
mRNA <1061564..>1063648
/gene="FRE5"
/locus_tag="YOR384W"
/product="putative ferric-chelate reductase"
/transcript_id="NM_001183804.1"
/db_xref="GeneID:854566"
CDS 1061564..1063648
/gene="FRE5"
/locus_tag="YOR384W"
/EC_number="1.16.1.9"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/note="Putative ferric reductase with similarity to Fre2p;
expression induced by low iron levels; the authentic,
non-tagged protein is detected in highly purified
mitochondria in high-throughput studies"
/codon_start=1
/product="putative ferric-chelate reductase"
/protein_id="NP_015029.1"
/db_xref="GeneID:854566"
/db_xref="SGD:S000005911"
/translation="MLFARLVLLLVYLAPGSLAKPASTKKRTQWDQIAIDACAKELES
HKFDTDVKGRHATLCTYEPALGSWLHCAKDVLDSRKKSKKIFEKTFSKINQYCHDYHK
DEVVSNEEYYRIFANASLFIRPLDEVKENIRYPVTPNKASLDRWVWAYFGPLDNIDKG
NVYGVTICLYWIGVLFIAAVYHFLNFSRLKQTVFKNKVSAFLRGHYVLPALVHNHAMS
VGRWFFIGLVPTRLETLVLFGYVLLHGFLLSSYNFDHNELLSDRRSQVLIFLSDRAGI
LAFAHFPLIVLFGGKNSTMTWLTGIRYTAFITYHKWLGRFMLVDCTIHAIGYTYHAYI
ENYWKYVKYSDLWTSGRHAMIIVGILVFFSFFFFRRHYYELFVITHIILAIGFFHACW
KHCYKLGWGEWIMACALFWIADRILRLIKIAIFGMPWAKLKLCGESMIEVRISKSSKW
WKAEPGQYIYLYFLRPKIFWQSHPFTVMDSLVEDGELVVVITVKNGLTKKLQEYLLES
EGYTEMRVLAEGPYGQSTRTHLFESLLFIAGGAGVPGPLSMAIKAGRQVKSNDSHQMI
KFVWSVRNLDLLEVYRKEIMVLKELNIDTKIYFTGERKDESNTEEGAIANMSTEGRLL
TTSKSAEMITDFGRPNIDEIIEEAVSGAKSLLVTCCGSEGFVDKTRELTAKRVLEHGD
KWIEYVEEFQNW"
gene <1065043..>1065915
/locus_tag="YOR385W"
/db_xref="GeneID:854567"
mRNA <1065043..>1065915
/locus_tag="YOR385W"
/product="uncharacterized protein"
/transcript_id="NM_001183805.3"
/db_xref="GeneID:854567"
CDS 1065043..1065915
/locus_tag="YOR385W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to the cytoplasm; YOR385W
is not an essential gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015030.3"
/db_xref="GeneID:854567"
/db_xref="SGD:S000005912"
/translation="MRGFSGQPLSDDDNYRIEKTQRNTIPERLHFSRERNMPIASIFG
TRGYFVFSSEQSYDKFKQTNFNISTLDADGVGVPLFHIVQSYNVIGKITRSSPDFYIY
KYVLQGVQDPPLYSDCKVICQDKVFRLCKILYCEIYAHQGFFETKYDFFYPSKTQPVK
KYQIIKQSNMRDLYSTLDGMRFRWHVKFYSDHFRLMFLDEDRLNYSNSNQKERQKPDQ
GKSKAPDFVIGHYTRTFSDILPRSTSKCSNLIIGEHSKPDSLGITTVPDLTQEFACQG
ALIHYLLHIERERK"
gene <1066839..>1068536
/gene="PHR1"
/locus_tag="YOR386W"
/db_xref="GeneID:854568"
mRNA <1066839..>1068536
/gene="PHR1"
/locus_tag="YOR386W"
/product="deoxyribodipyrimidine photo-lyase PHR1"
/transcript_id="NM_001183806.1"
/db_xref="GeneID:854568"
CDS 1066839..1068536
/gene="PHR1"
/locus_tag="YOR386W"
/EC_number="4.1.99.3"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:21124907]"
/experiment="EXISTENCE:direct assay:GO:0003904
deoxyribodipyrimidine photo-lyase activity [PMID:2911265]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/note="DNA photolyase involved in photoreactivation;
repairs pyrimidine dimers in the presence of visible
light; induced by DNA damage; regulated by transcriptional
repressor Rph1p"
/codon_start=1
/product="deoxyribodipyrimidine photo-lyase PHR1"
/protein_id="NP_015031.1"
/db_xref="GeneID:854568"
/db_xref="SGD:S000005913"
/translation="MKRTVISSSNAYASKRSRLDIEHDFEQYHSLNKKYYPRPITRTG
ANQFNNKSRAKPMEIVEKLQKKQKTSFENVSTVMHWFRNDLRLYDNVGLYKSVALFQQ
LRQKNAKAKLYAVYVINEDDWRAHMDSGWKLMFIMGALKNLQQSLAELHIPLLLWEFH
TPKSTLSNSKEFVEFFKEKCMNVSSGTGTIITANIEYQTDELYRDIRLLENEDHRLQL
KYYHDSCIVAPGLITTDRGTNYSVFTPWYKKWVLYVNNYKKSTSEICHLHIIEPLKYN
ETFELKPFQYSLPDEFLQYIPKSKWCLPDVSEEAALSRLKDFLGTKSSKYNNEKDMLY
LGGTSGLSVYITTGRISTRLIVNQAFQSCNGQIMSKALKDNSSTQNFIKEVAWRDFYR
HCMCNWPYTSMGMPYRLDTLDIKWENNPVAFEKWCTGNTGIPIVDAIMRKLLYTGYIN
NRSRMITASFLSKNLLIDWRWGERWFMKHLIDGDSSSNVGGWGFCSSTGIDAQPYFRV
FNMDIQAKKYDPQMIFVKQWVPELISSENKRPENYPKPLVDLKHSRERALKVYKDAM"
gene complement(<1069621..>1070241)
/locus_tag="YOR387C"
/db_xref="GeneID:854569"
mRNA complement(<1069621..>1070241)
/locus_tag="YOR387C"
/product="uncharacterized protein"
/transcript_id="NM_001183807.3"
/db_xref="GeneID:854569"
CDS complement(1069621..1070241)
/locus_tag="YOR387C"
/note="hypothetical protein; regulated by the
metal-responsive Aft1p transcription factor; highly
inducible in zinc-depleted conditions; localizes to the
soluble fraction; SWAT-GFP and mCherry fusion proteins
localize to the endoplasmic reticulum and vacuole
respectively; YOR387C has a paralog, VEL1, that arose from
a single-locus duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015032.3"
/db_xref="GeneID:854569"
/db_xref="SGD:S000005914"
/translation="MSFLNIFTFFSVLVSVATAVRFDLTNVTCNNLHGPHCGTYVMEV
VGQNGTFLGQSTFAGADVLTESAGDAWARYLGQETRFLPKLTTIASNDTKNFSPLIFT
TNIYTCNPQSIGDAMVPFANTVTGEIEYNSWADTADNASFITGLANQLFNSTQYGVQV
ASCYPNFASVILSTPTVNIFAANETLPDYCTAIQLKAVCPPDAGFA"
gene complement(<1071793..>1072923)
/gene="FDH1"
/locus_tag="YOR388C"
/db_xref="GeneID:854570"
mRNA complement(<1071793..>1072923)
/gene="FDH1"
/locus_tag="YOR388C"
/product="formate dehydrogenase (NAD+)"
/transcript_id="NM_001183808.1"
/db_xref="GeneID:854570"
CDS complement(1071793..1072923)
/gene="FDH1"
/locus_tag="YOR388C"
/EC_number="1.17.1.9"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:11921099]"
/experiment="EXISTENCE:direct assay:GO:0008863 formate
dehydrogenase (NAD+) activity [PMID:21246355]"
/experiment="EXISTENCE:genetic interaction:GO:0042183
formate catabolic process [PMID:19564482]"
/note="NAD(+)-dependent formate dehydrogenase; may protect
cells from exogenous formate"
/codon_start=1
/product="formate dehydrogenase (NAD+)"
/protein_id="NP_015033.1"
/db_xref="GeneID:854570"
/db_xref="SGD:S000005915"
/translation="MSKGKVLLVLYEGGKHAEEQEKLLGCIENELGIRNFIEEQGYEL
VTTIDKDPEPTSTVDRELKDAEIVITTPFFPAYISRNRIAEAPNLKLCVTAGVGSDHV
DLEAANERKITVTEVTGSNVVSVAEHVMATILVLIRNYNGGHQQAINGEWDIAGVAKN
EYDLEDKIISTVGAGRIGYRVLERLVAFNPKKLLYYDYQELPAEAINRLNEASKLFNG
RGDIVQRVEKLEDMVAQSDVVTINCPLHKDSRGLFNKKLISHMKDGAYLVNTARGAIC
VAEDVAEAVKSGKLAGYGGDVWDKQPAPKDHPWRTMDNKDHVGNAMTVHISGTSLDAQ
KRYAQGVKNILNSYFSKKFDYRPQDIIVQNGSYATRAYGQKK"
gene <1074213..>1076087
/locus_tag="YOR389W"
/db_xref="GeneID:854571"
mRNA <1074213..>1076087
/locus_tag="YOR389W"
/product="uncharacterized protein"
/transcript_id="NM_001183809.3"
/db_xref="GeneID:854571"
CDS 1074213..1076087
/locus_tag="YOR389W"
/note="hypothetical protein; expression regulated by
copper levels"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015034.3"
/db_xref="GeneID:854571"
/db_xref="SGD:S000005916"
/translation="MRFHRQGTAATVGVLLIVLLGFCWKLSESYGIVSTALPHKQPAT
KITDTPSIRWDNYHEFVRDIDFDNSTAIFNSIRAALRQSPSDIHPVGVSYFPAVIPKG
TLMYHAGSKVPTTFEWLAMDHEFSYSFGLRSPSYGRKSLERRHGRFGNGTHGDHPKGP
PPPPPPDEKDRGSQKMLTYRAARDLNKFLYLDGASAAKTDSGEMDTQLMLSNVIKEKL
NLTDDGENERMAERLYAARICKWGKPFGLDGIIRVEVGFEVVLCDFSADNVELVSMLE
MVQPNQYLGLPAPTVISKEEGWPLDENGNLVEDQLTDDQKAILEREDGWEKTFSNFNA
VKSFNQLRAGTAHDNGEHRIHIDYRYLVSGINRTYIAPDPNNRRLLDEGMTWEKQLDM
VDDLEKALEVGFDATQSMDWQLAFDELVLKFAPLLKSVSNILNSNGDINESIAINATA
LTLNFCLRFEPASNNSDEFGSGKDFAVYQYVSPYQALKTDADFLIWSSAVSVVGEIVD
AIYKVNDLLIPEVYSFMTDNTTSSDLIKNVETARSTIDGLIESLGWIELNYRCERQCN
WDEVCYTPSWGPSPMGMTEPGSHNEGFGTHFDESRQRLVINSKLQCININDLMVNRNH
"
gene <1076784..>1077911
/gene="FEX1"
/locus_tag="YOR390W"
/db_xref="GeneID:854572"
mRNA <1076784..>1077911
/gene="FEX1"
/locus_tag="YOR390W"
/product="fluoride transporter"
/transcript_id="NM_001183810.1"
/db_xref="GeneID:854572"
CDS 1076784..1077911
/gene="FEX1"
/locus_tag="YOR390W"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:26055717]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:1903424
fluoride transmembrane transport
[PMID:24173035|PMID:26055717]"
/experiment="EXISTENCE:genetic interaction:GO:1903425
fluoride transmembrane transporter activity
[PMID:24173035]"
/experiment="EXISTENCE:mutant phenotype:GO:1903424
fluoride transmembrane transport [PMID:26055717]"
/note="Protein involved in fluoride export; nearly
identical to FEX2, and deletion of both proteins results
in a large increase in fluoride sensitivity compared with
the single mutant; contains two FEX domains connected by a
linker; part of a widespread family of conserved fluoride
export proteins"
/codon_start=1
/product="fluoride transporter"
/protein_id="NP_015035.1"
/db_xref="GeneID:854572"
/db_xref="SGD:S000005917"
/translation="MIFNPVISNHKLSHYIHVFCTFTTFCILGTETRQAITALSTYTP
AFVTAPTVLWSNCSSCMLMGIMQSLNAYTWMKDHQVLFLGVTTGYCGALSSFSSMLLE
MFEHSTNLTNGNIANHTKLPNRAYGIMEFLSVLLVHLMVSMGSLIFGRQLGKEVIVAY
GSSSFSKPYTPPSDTVKENAGDVDTQEMEKNILEFKFKTPAPFFKKFFDIVDKLAYAL
AFPLIILFVVLCAYYENYSRGKWTLPCLFGIFAGFLRYWLAEMFNKTNKKFPLGTFLA
NVFATLLIGIFTMVQRGKKHFSTDVPIVNSLNSCHIVSALISGFCGTLSTISTFINEG
YKLSFINMLIYYTVSIAISYCLLVITLGSYAWTRGLTNPIC"
gene complement(<1078545..>1079258)
/gene="HSP33"
/locus_tag="YOR391C"
/db_xref="GeneID:854573"
mRNA complement(<1078545..>1079258)
/gene="HSP33"
/locus_tag="YOR391C"
/product="glutathione-independent methylglyoxalase family
protein"
/transcript_id="NM_001183811.3"
/db_xref="GeneID:854573"
CDS complement(1078545..1079258)
/gene="HSP33"
/locus_tag="YOR391C"
/EC_number="4.2.1.130"
/experiment="EXISTENCE:mutant phenotype:GO:0031669
cellular response to nutrient levels [PMID:24706893]"
/note="Possible chaperone and cysteine protease; required
for transcriptional reprogramming during the diauxic shift
and for survival in stationary phase; similar to E. coli
Hsp31 and S. cerevisiae Hsp31p, Hsp32p, and Sno4p; member
of the DJ-1/ThiJ/PfpI superfamily, which includes human
DJ-1 involved in Parkinson's disease and cancer"
/codon_start=1
/product="glutathione-independent methylglyoxalase family
protein"
/protein_id="NP_015036.3"
/db_xref="GeneID:854573"
/db_xref="SGD:S000005918"
/translation="MTPKRALISLTSYHGPFYKDGAKTGVFVVEILRSFDTFEKHGFE
VDFVSETGGFGWDEHYLPKSFIGGEDKMNFETKNSAFNKALARIKTANEVNASDYKVF
FASAGHGALFDYPKAKNLQDIASKIYANGGVIAAICHGPLLFDGLIDIKTTRPLIEGK
AITGFPLEGEIALGVDDILRSRKLTTVERVANKNGAKYLAPIHPWDDYSITDGKLVTG
VNANSSYSTTIRAINALYS"
gene <1080276..>1081589
/gene="ERR1"
/locus_tag="YOR393W"
/db_xref="GeneID:854575"
mRNA <1080276..>1081589
/gene="ERR1"
/locus_tag="YOR393W"
/product="phosphopyruvate hydratase ERR1"
/transcript_id="NM_001183813.1"
/db_xref="GeneID:854575"
CDS 1080276..1081589
/gene="ERR1"
/locus_tag="YOR393W"
/EC_number="4.2.1.11"
/note="Putative phosphopyruvate hydratase"
/codon_start=1
/product="phosphopyruvate hydratase ERR1"
/protein_id="NP_015038.1"
/db_xref="GeneID:854575"
/db_xref="SGD:S000005920"
/translation="MSITKVHARTVYDSRGNPTVEVEITTENGLFRAIVPSGASTGIH
EAVELRDGNKSEWMGKGVTKAVSNVNSIIGPALIKSELCVTNQKGIDELMISLDGTSN
KSRLGANAILGVSLCVARAAAAQKGITLYKYIAELADARQDPFVIPVPFFNVLNGGAH
AGGSLAMQEFKIAPVGAQSFAEAMRMGSEVYHHLKILAKEQYGPSAGNVGDEGGVAPD
IDTAEDALDMIVEAINICGYEGRVKVGIDSAPSVFYKDGKYDLNFKEPNSDPSHWLSP
AQLAEYYHSLLKKYPIISLEDPYAEDDWSSWSAFLKTVNVQIIADDLTCTNKTRIARA
IEEKCANTLLLKLNQIGTLTESIEAANQAFDAGWGVMISHRSGETEDPFIADLVVGLR
CGQIKSGALSRSERLAKYNELLRIEEELGDDCIYAGHRFHDGNKL"
gene <1082718..>1083212
/gene="PAU21"
/locus_tag="YOR394W"
/db_xref="GeneID:854576"
mRNA <1082718..>1083212
/gene="PAU21"
/locus_tag="YOR394W"
/product="seripauperin PAU21"
/transcript_id="NM_001183814.3"
/db_xref="GeneID:854576"
CDS 1082718..1083212
/gene="PAU21"
/locus_tag="YOR394W"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/note="hypothetical protein; member of the seripauperin
multigene family encoded mainly in subtelomeric regions;
SWAT-GFP, seamless-GFP and mCherry fusion proteins
localize to the cytosol; identical to Pau22p; encodes two
proteins that are translated from 2 different start
codons"
/codon_start=1
/product="seripauperin PAU21"
/protein_id="NP_015039.3"
/db_xref="GeneID:854576"
/db_xref="SGD:S000005921"
/translation="MTNEGIGINRDTSTICLREYVFIHFFPVKLISALTNKTNTMVKL
TSIAAGVAAIAAGVAAAPATTTLSPSDERVNLVELGVYVSDIRAHLAQYYLFQAAHPT
ETYPVEIAEAVFNYGDFTTMLTGIPAEQVTRVITGVPWYSTRLRPAISSALSKDGIYT
AIPK"
telomere 1083922..1091291
/note="TEL15R; Telomeric region on the right arm of
Chromosome XV; composed of an X element core sequence, X
element combinatorial repeats, a long Y' element, and a
very short terminal stretch of telomeric repeats"
/db_xref="SGD:S000028997"
gene complement(<1084202..>1084369)
/locus_tag="YOR394C-A"
/db_xref="GeneID:1466490"
mRNA complement(<1084202..>1084369)
/locus_tag="YOR394C-A"
/product="uncharacterized protein"
/transcript_id="NM_001184626.1"
/db_xref="GeneID:1466490"
CDS complement(1084202..1084369)
/locus_tag="YOR394C-A"
/note="hypothetical protein; identified by gene-trapping,
microarray-based expression analysis, and genome-wide
homology searching"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878178.1"
/db_xref="GeneID:1466490"
/db_xref="SGD:S000028718"
/translation="MIVNNTHILTLPPHAVSTLTCILIWHRHTDATVYIISSYPTLTF
HSMAHLSLHQY"
gene <1085473..>1090863
/gene="YRF1-8"
/locus_tag="YOR396W"
/db_xref="GeneID:854577"
mRNA <1085473..>1090863
/gene="YRF1-8"
/locus_tag="YOR396W"
/product="Y' element ATP-dependent helicase protein 1 copy
8"
/transcript_id="NM_001184447.3"
/db_xref="GeneID:854577"
CDS 1085473..1090863
/gene="YRF1-8"
/locus_tag="YOR396W"
/note="One of several telomeric Y' element-encoded DNA
helicases; known as Y'-Help1 (Y'-HELicase Protein 1)"
/codon_start=1
/product="Y' element ATP-dependent helicase protein 1 copy
8"
/protein_id="NP_061495.4"
/db_xref="GeneID:854577"
/db_xref="SGD:S000007526"
/translation="MKVSDRRKFEKANFDEFESALNNKNDLVHCPSITLFESIPTEVR
SFYEDEKSGLIKVVKFRTGAMDRKRSFEKIVISVMVGKNVQKFLTFVEDEPDFQGGPI
PSKYLIPKKINLMVYTLFQVHTLKFNRKDYDTLSLFYLNRGYYNELSFRVLERCHEIA
SARPNDSSTMRTFTDFVSGAPIVRSLQKSTIRKYGYNLAPYMFLLLHVDELSIFSAYQ
ASLPGEKKVDTERLKRDLCPRKPIEIKYFSQICNDMMNKKDRLGDILHIILRACALNF
GAGPRGGAGDEEDRSITNEEPIIPSVDEHGLKVCKLRSPNTPRRLRKTLDAVKALLVS
SCACTARDLDIFDDTNGVAMWKWIKILYHEVAQETTLKDSYRITLVPSSDGISVCGKL
FNREYVRGFYFACKAQFDNLWGELNNCFYMPTVVDIASLILRNREVLFREPKRGIDEY
LENDSFLQMIPVKYREIVLPKLRRDTNKMTAALKNKVTVAIDELTVPLMWMVHFAVGY
PYRYPELQLLAFAGPQRNVYVDDTTRRIQLYTDYNKNGSSEPRLKTLDGLTSDYVFYF
VTVLRQMQICALGNSYDAFNHDPWMDVVGFEDPDQVTNRDISRIVLYSYMFLNTAKGC
LVEYATFRQYMRELPKNAPQKLNFREMRQGLIALGRHCVGSRFETDLYESATSELMAN
HSVQTGRNIYGVDSFSLTSVSGTTATLLQERASERWIQWLGLESDYHCSFSSTRNAED
VVAGEAASSDHDQKISRVTRKRPREPKSTNDILVAGQKLFGSSFEFRDLHQLRLCHEI
YMADTPSVAVQAPPGYGKTELFHLPLIALASKGDVKYVSFLFVPYTVLLANCMIRLSR
CGCLNVAPVRNFIEEGCDGVTDLYVGIYDDLASTNFTDRIAAWENIVECTFRTNNVKL
GYLIVDEFHNFETEVYRQSQFGGITNLDFDAFEKAIFLSGTAPEAVADAALQRIGLTG
LAKKSMDINELKRSEDLSRGLSSYPTRMFNLIKEKSEVPLGHVHKIWKKVESQPEEAL
KLLLALFEIEPESKAIVVASTTNEVEELACSWRKYFRVVWIHGKLGAAEKVSRTKEFV
TDGSMRVLIGTKLVTEGIDIKQLMMVIMLDNRLNIIELIQGVGRLRDGGLCYLLSRKN
SWAARNRKGELPPIKEGCITEQVREFYGLESKKGKKGQHVGCCGSRTDLSADTVELIE
RMDRLAEKQATASMSIIALPSSFQESNSSDRCRKYCSSDEDSDTCIHGSANASTNATT
NSSTNATTTASTNVRTSATTTASINVRTSAITTESTNSSTNATTTASTNVRTSATTTA
SINVRTSATTTESTNSNTSATTTESTDSNTSATTTESTDSNTSATTTASTNSSTNATT
TASTNSSTNATTTESTNASAKEDANKDGNAEDNRFHPVTDINKESYKRKGSQMVLLER
KKLKAQFPNTSENMNVLQFLGFRSDEIKHLFLYGIDVYFCPEGVFTQYGLCKGCQKMF
ELCVCWAGQKVSYRRMAWEALAVERMLRNDEEYKEYLEDIEPYHGDPVGYLKYFSVKR
GEIYSQIQRNYAWYLAITRRRETISVLDSTRGKQGSQVFRMSGRQIKELYYKVWSNLR
ESKTEVLQYFLNWDEKKCREEWEAKDDTVFVEALEKVGVFQRLRSMTSAGLQGPQYVK
LQFSRHHRQLRSRYELSLGMHLRDQLALGVTPSKVPHWTAFLSMLIGLFYNKTFRQKL
EYLLEQISEVWLLPHWLDLANVEVLAADNTRVPLYMLMVAVHKELDSDDVPDGRFDII
LLCRDSSREVGE"
CONTIG join(BK006948.2:1..1091291)
//
