LOCUS NC_001148 948066 bp DNA linear CON 23-JAN-2026
DEFINITION Saccharomyces cerevisiae S288C chromosome XVI, complete sequence.
ACCESSION NC_001148
VERSION NC_001148.4
DBLINK BioProject: PRJNA128
Assembly: GCF_000146045.2
KEYWORDS RefSeq.
SOURCE Saccharomyces cerevisiae S288C
ORGANISM Saccharomyces cerevisiae S288C
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
Saccharomyces.
REFERENCE 1 (bases 1 to 948066)
AUTHORS Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M.,
Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S.,
Weng,S. and Cherry,J.M.
TITLE New data and collaborations at the Saccharomyces Genome Database:
updated reference genome, alleles, and the Alliance of Genome
Resources
JOURNAL Genetics 220 (4) (2022)
PUBMED 34897464
REFERENCE 2 (bases 1 to 948066)
AUTHORS Bussey,H., Storms,R.K., Ahmed,A., Albermann,K., Allen,E.,
Ansorge,W., Araujo,R., Aparicio,A., Barrell,B., Badcock,K.,
Benes,V., Botstein,D., Bowman,S., Bruckner,M., Carpenter,J.,
Cherry,J.M., Chung,E., Churcher,C., Coster,F., Davis,K.,
Davis,R.W., Dietrich,F.S., Delius,H., DiPaolo,T., Hani,J. et al.
TITLE The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI
JOURNAL Nature 387 (6632 SUPPL), 103-105 (1997)
PUBMED 9169875
REFERENCE 3 (bases 1 to 948066)
AUTHORS Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
Oliver,S.G.
TITLE Life with 6000 genes
JOURNAL Science 274 (5287), 546 (1996)
PUBMED 8849441
REFERENCE 4 (bases 1 to 948066)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (23-JAN-2026) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 5 (bases 1 to 948066)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (16-JAN-2015) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Protein update by submitter
REFERENCE 6 (bases 1 to 948066)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (31-MAR-2011) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Sequence update by submitter
REFERENCE 7 (bases 1 to 948066)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (14-DEC-2009) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
COMMENT REVIEWED REFSEQ: This record has been curated by SGD. The reference
sequence is identical to BK006949.
On Apr 26, 2011 this sequence version replaced NC_001148.3.
##Genome-Annotation-Data-START##
Annotation Provider :: SGD
Annotation Status :: Full Annotation
Annotation Version :: R64-4-1
URL :: http://www.yeastgenome.org/
##Genome-Annotation-Data-END##
COMPLETENESS: full length.
FEATURES Location/Qualifiers
source 1..948066
/organism="Saccharomyces cerevisiae S288C"
/mol_type="genomic DNA"
/strain="S288C"
/db_xref="taxon:559292"
/chromosome="XVI"
telomere complement(1..7223)
/note="TEL16L; Telomeric region on the left arm of
Chromosome XVI; annotated components include an X element
core sequence, X element combinatorial repeats, and a long
Y' element; TEL16L does have telomeric repeats
(TEL16L-TR), but they are missing from the genome
annotation due to difficulties encountered during
sequencing and/or assembly"
/db_xref="SGD:S000028933"
gene complement(<280..>6007)
/gene="YRF1-7"
/locus_tag="YPL283C"
/gene_synonym="YRF1"
/db_xref="GeneID:855846"
mRNA complement(join(<280..5840,5989..>6007))
/gene="YRF1-7"
/locus_tag="YPL283C"
/gene_synonym="YRF1"
/product="Y' element ATP-dependent helicase protein 1 copy
7"
/transcript_id="NM_001184097.1"
/db_xref="GeneID:855846"
CDS complement(join(280..5840,5989..6007))
/gene="YRF1-7"
/locus_tag="YPL283C"
/gene_synonym="YRF1"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:genetic interaction:GO:0000722
telomere maintenance via recombination [PMID:9837911]"
/note="Helicase encoded by the Y' element of subtelomeric
regions; highly expressed in the mutants lacking the
telomerase component TLC1; potentially phosphorylated by
Cdc28p"
/codon_start=1
/product="Y' element ATP-dependent helicase protein 1 copy
7"
/protein_id="NP_015040.1"
/db_xref="GeneID:855846"
/db_xref="SGD:S000006204"
/translation="MEIENEQICTCIAQILHLLNSLIITFLDDDKTETGQSFVYIDGF
LVKKHNNQHTIVNFETYKNKMKVSDRRKFEKANFDEFESALNNKNDLVHCPSITLFES
IPTEVRSFYEDEKSGLIKVVKFRTGAMDRKRSFEKIVVSVMVGKNVQKFLTFVEDEPD
FQGGPIPSKYLIPKKINLMVYTLFQVHTLKFNRKDYDTLSLFYLNRGYYNELSFRVLE
RCYEIASARPNDSSTMRTFTDFVSGTPIVRGLQKSTIRKYGYNLAPYMFLLLHVDELS
IFSAYQASLPGEKKVDTERLKRDLCPRKPTEIKYFSQICNDMMNKKDRLGDILHIILR
ACALNFGAGPRGGAGDEEDRSITNEEPIIPSVDEHGLKVCKLRSPNTPRRLRKTLDAV
KALLVSSCACTARDLDIFDDNNGVAMWKWIKILYHEVAQETALKDSYRITLVPSSDGV
SVCGKLFNREYVRGFYFACKAQFDNLWEELNDCFYMPTVVDIASLILRNREVLFREPK
RGIDEYLENDSFLQMIPVKYREIVLPKLRRDTNKMTAALKNKVTVAIDELTVPLMWMI
HFAVGYPYRYPELQLLAFAGPQRNVYVDDTTRRIQLYTDYNKNGSSEPRLKTLDGLTS
DYVFYFVTVLRQMQICALGNSYDAFNHDPWMDVVGFEDPDQVTNRDISRIVLYSYMFL
NTAKGCLVEYATFRQYMRELPKNAPQKLNFREMRQGLIALGRHCVGSRFETDLYESAT
SELMANHSVQTGRNIYGVDSFSLTSVSGTTATLLQERASERWIQWLGLESDYHCSFSS
TRNAEDVVAGEAASSDHHQKISRVTRKRPREPKSTNDILVAGQKLFGSSFEFRDLHQL
RLCHEIYMADTPSVAVQAPPGYGKTELFHLPLIALASKGDVKYVSFLFVPYTVLLANC
MIRLSRCGCLNVAPVRNFIEEGCDGVTDLYVGIYDDLASTNFTDRIAAWENIVECTFR
TNNVKLGYLIVDEFHNFETEVYRQSQFGGITNLDFDAFEKAIFLSGTAPEAVADAALQ
RIGLTGLAKKSMDINELKRSEDLSRGLSSYPTRMFNLIKEKSEVPLGHVHKIWKKVES
QPEEALKLLLALFEIEPESKAIVVASTTNEVEELACSWRKYFRVVWIHGKLGAAEKVS
RTKEFVTDGSMRVLIGTKLVTEGIDIKQLMMVIMLDNRLNIIELIQGVGRLRDGGLCY
LLSRKNSWAARNRKGELPPIKEGCITEQVREFYGLESKKGKKGQHVGCCGSRTDLSAD
TVELIERMDRLAEKQATASMSIVALPSSFQESNSSDRCRKYCSSDEDSDTCIHGSANA
STNATTNSSTNATTTASTNVRTSATTTASINVRTSATTTESTNSSTNATTTASTNVRT
SATTTASINVRTSATTTESTNSNTSATTTESTDSNTSATTTESTDSNTSATTTASTNS
STNATTTASTNSSTNATTTESTNASAKEDANKDGNAEDNRFHPVTDINKESYKRKGSQ
MVLLERKKLKAQFPNTSENMNVLQFLGFRSDEIKHLFLYGIDVYFCPEGVFTQYGLCK
GCQKMFELCVCWAGQKVSYRRMAWEALAVERMLRNDEEYKEYLEDIEPYHGDPVGYLK
YFSVKRGEIYSQIQRNYAWYLAITRRRETISVLDSTRGKQGSQVFRMSGRQIKELYYK
VWSNLRESKTEVLQYFLNWDEKKCREEWEAKDDTVFVEALEKVGVFQRLRSMTSAGLQ
GPQYVKLQFSRHHRQLRSRYELSLGMHLRDQLALGVTPSKVPHWTAFLSMLIGLFCNK
TFRQKLEYLLEQISEVWLLPHWLDLANVEVLAADNTRVPLYMLMVAVHKELDSDDVPD
GRFDILLCRDSSREVGE"
gene complement(<7933..>8427)
/gene="PAU22"
/locus_tag="YPL282C"
/db_xref="GeneID:855847"
mRNA complement(<7933..>8427)
/gene="PAU22"
/locus_tag="YPL282C"
/product="seripauperin PAU22"
/transcript_id="NM_001184096.1"
/db_xref="GeneID:855847"
CDS complement(7933..8427)
/gene="PAU22"
/locus_tag="YPL282C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/note="hypothetical protein; member of the seripauperin
multigene family encoded mainly in subtelomeric regions;
SWAT-GFP fusion protein localizes to the endoplasmic
reticulum and vacuole, while mCherry fusion localizes to
just the vacuole; identical to Pau21p; encodes two
proteins that are translated from two different start
codons"
/codon_start=1
/product="seripauperin PAU22"
/protein_id="NP_015041.1"
/db_xref="GeneID:855847"
/db_xref="SGD:S000006203"
/translation="MTNEGIGINRDTSTICLREYVFIHFFPVKLISALTNKTNTMVKL
TSIAAGVAAIAAGVAAAPATTTLSPSDERVNLVELGVYVSDIRAHLAQYYLFQAAHPT
ETYPVEIAEAVFNYGDFTTMLTGIPAEQVTRVITGVPWYSTRLRPAISSALSKDGIYT
AIPK"
gene complement(<9557..>10870)
/gene="ERR2"
/locus_tag="YPL281C"
/db_xref="GeneID:855848"
mRNA complement(<9557..>10870)
/gene="ERR2"
/locus_tag="YPL281C"
/product="phosphopyruvate hydratase ERR2"
/transcript_id="NM_001184095.1"
/db_xref="GeneID:855848"
CDS complement(9557..10870)
/gene="ERR2"
/locus_tag="YPL281C"
/EC_number="4.2.1.11"
/experiment="EXISTENCE:direct assay:GO:0004634
phosphopyruvate hydratase activity [PMID:23359425]"
/experiment="EXISTENCE:genetic interaction:GO:0004634
phosphopyruvate hydratase activity [PMID:23359425]"
/note="Enolase, a phosphopyruvate hydratase; catalyzes the
conversion of 2-phosphoglycerate to phosphoenolpyruvate;
complements the growth defect of an ENO1 ENO2 double
mutant"
/codon_start=1
/product="phosphopyruvate hydratase ERR2"
/protein_id="NP_015042.1"
/db_xref="GeneID:855848"
/db_xref="SGD:S000006202"
/translation="MSITKVHARTVYDSRGNPTVEVEITTENGLFRAIVPSGASTGIH
EAVELRDGNKSEWMGKGVTKAVSNVNSIIGPALIKSELCVTNQKGIDELMISLDGTSN
KSRLGANAILGVSLCVARAAAAQKGITLYKYIAELADARQDPFVIPVPFFNVLNGGAH
AGGSLAMQEFKIAPVGAQSFAEAMRMGSEVYHHLKILAKEQYGPSAGNVGDEGGVAPD
IDTAEDALDMIVEAINICGYEGRVKVGIDSAPSVFYKDGKYDLNFKEPNSDPSHWLSP
AQLAEYYHSLLKKYPIISLEDPYAEDDWSSWSAFLKTVNVQIIADDLTCTNKTRIARA
IEEKCANTLLLKLNQIGTLTESIEAANQAFDAGWGVMISHRSGETEDPFIADLVVGLR
CGQIKSGALSRSERLAKYNELLRIEEELGDDCIYAGHRFHDGNKL"
gene <11887..>12600
/gene="HSP32"
/locus_tag="YPL280W"
/db_xref="GeneID:855849"
mRNA <11887..>12600
/gene="HSP32"
/locus_tag="YPL280W"
/product="glutathione-independent methylglyoxalase family
protein"
/transcript_id="NM_001184094.1"
/db_xref="GeneID:855849"
CDS 11887..12600
/gene="HSP32"
/locus_tag="YPL280W"
/EC_number="4.2.1.130"
/experiment="EXISTENCE:direct assay:GO:0000932 P-body
[PMID:24706893]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:24706893|PMID:26777405]"
/experiment="EXISTENCE:genetic interaction:GO:0051596
methylglyoxal catabolic process [PMID:27984092]"
/experiment="EXISTENCE:mutant phenotype:GO:0031669
cellular response to nutrient levels [PMID:24706893]"
/note="Possible chaperone and cysteine protease; required
for transcriptional reprogramming during the diauxic shift
and for survival in stationary phase; similar to E. coli
Hsp31 and S. cerevisiae Hsp31p, Hsp33p, and Sno4p; member
of the DJ-1/ThiJ/PfpI superfamily, which includes human
DJ-1 involved in Parkinson's disease and cancer"
/codon_start=1
/product="glutathione-independent methylglyoxalase family
protein"
/protein_id="NP_015043.1"
/db_xref="GeneID:855849"
/db_xref="SGD:S000006201"
/translation="MTPKRALISLTSYHGPFYKDGAKTGVFVVEILRSFDTFEKHGFE
VDFVSETGGFGWDEHYLPKSFIGGEDKMNFETKNSAFNKALARIKTANEVNASDYKIF
FASAGHGALFDYPKAKNLQDIASKIYANGGVIAAICHGPLLFDGLIDIKTTRPLIEGK
AITGFPLEGEIALGVDDILRSRKLTTVERVANKNGAKYLAPIHPWDDYSITDGKLVTG
VNANSSYSTTIRAINALYS"
gene complement(<13228..>14355)
/gene="FEX2"
/locus_tag="YPL279C"
/db_xref="GeneID:855850"
mRNA complement(<13228..>14355)
/gene="FEX2"
/locus_tag="YPL279C"
/product="fluoride transporter"
/transcript_id="NM_001184093.1"
/db_xref="GeneID:855850"
CDS complement(13228..14355)
/gene="FEX2"
/locus_tag="YPL279C"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:26055717]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:1903424
fluoride transmembrane transport
[PMID:24173035|PMID:26055717]"
/experiment="EXISTENCE:genetic interaction:GO:1903425
fluoride transmembrane transporter activity
[PMID:24173035]"
/experiment="EXISTENCE:mutant phenotype:GO:1903424
fluoride transmembrane transport [PMID:26055717]"
/note="Protein involved in fluoride export; nearly
identical to FEX1, and deletion of both proteins results
in a large increase in fluoride sensitivity compared with
the single mutant; contains two FEX domains connected by a
linker; part of a widespread family of conserved fluoride
export proteins"
/codon_start=1
/product="fluoride transporter"
/protein_id="NP_015044.1"
/db_xref="GeneID:855850"
/db_xref="SGD:S000006200"
/translation="MIFNPVISNHKLSHYIHVFCTFTTFCILGTETRQAITALSTYTP
AFVTAPTVLWSNCSSCMLMGIMQSLNAYTWMKDHQVLFLGVTTGYCGALSSFSSMLLE
MFEHSTNLTNGNIANHTKLPNRAYGIMEFLSVLLVHLMVSMGSLIFGRQLGKEVIVAY
GSSSFSKPYTPPSDTVKENAGDVDTQEMEKNILEFKFKTPAPFFKKFFDVVDKLAYAL
AFPLIILFVVLCAYYENYSRGKWTLPCLFGIFAGFLRYWLAEMFNKTNKKFPLGTFLA
NVFATLLIGIFTMVQRGKKHFSTDIPIVNSLNSCHIVSALISGFCGTLSTISTFINEG
YKLSFINMLIYYTVSIGISYCLLVITLGSYAWTRGLTNPIC"
gene complement(<15053..>15355)
/locus_tag="YPL278C"
/db_xref="GeneID:855851"
mRNA complement(<15053..>15355)
/locus_tag="YPL278C"
/product="uncharacterized protein"
/transcript_id="NM_001184092.1"
/db_xref="GeneID:855851"
CDS complement(15053..15355)
/locus_tag="YPL278C"
/note="hypothetical protein; gene expression regulated by
copper levels"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015045.1"
/db_xref="GeneID:855851"
/db_xref="SGD:S000006199"
/translation="MTDNTTSSDLIKNVETARSTIDGLIESLGWIELNYRCERQCNWD
EVCYTPSWGPSPMGMTEPGSHNEGFGTHFDESRQRLVINSKLQCININDLMVNRNH"
gene complement(<15405..>16868)
/locus_tag="YPL277C"
/db_xref="GeneID:855852"
mRNA complement(<15405..>16868)
/locus_tag="YPL277C"
/product="uncharacterized protein"
/transcript_id="NM_001184091.1"
/db_xref="GeneID:855852"
CDS complement(15405..16868)
/locus_tag="YPL277C"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:11935221]"
/note="hypothetical protein; localized to the membranes;
gene expression regulated by copper levels"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015046.1"
/db_xref="GeneID:855852"
/db_xref="SGD:S000006198"
/translation="MRFHRQGISAIIGVLLIVLLGFCWKLSGSYGIVSTALPHNQSAI
KSTDLPSIRWDNYHEFVRDIDFDNSTAIFNSIRAALRQSPSDIHPVGVSYFPAVIPKG
TLMYHAGSKVPTTFEWLAMDHEFSYSFGLRSPSYGRKSLERRHGRFGNGTNGDHPKGP
PPPPPPPDEKGRGSQKMLTYRAARDLNKFLYLDGASAAKTDSGEMDTQLMLSNVIKEK
LNLTDDGENERMAERLYAARICKWGKPFGLDGIIRVEVGFEVVLCDFSADNVELVSML
EMVQPNQYLGLPAPTVISKEEGWPLDENGSLVEDQLTDDQKAILEREDGWEKAFSNFN
AVKSFNQLRAGAAHDNGEHRIHIDYRYLVSGINRTYIAPDPNNRRLLDEGMTWEKQLD
MVDDLEKALEVGFDATQSMDWQLAFDELVLKFAPLLKSVSNILNSDGDINESIAINAT
ALTLNFCLPICEPIPGLKNGCRLFDLVICCQRCRRNC"
gene 17948..18385
/locus_tag="YPL276W"
/gene_synonym="FDH2"
/note="NAD(+)-dependent formate dehydrogenase; may protect
cells from exogenous formate; YPL275W and YPL276W comprise
a continuous open reading frame in some S. cerevisiae
strains but not in the genomic reference strain S288C"
/pseudo
/db_xref="GeneID:2827705"
/db_xref="SGD:S000006197"
mRNA 17948..18385
/locus_tag="YPL276W"
/gene_synonym="FDH2"
/pseudo
/db_xref="GeneID:2827705"
/db_xref="SGD:S000006197"
gene 18369..19079
/gene="FDH2"
/locus_tag="YPL275W"
/note="NAD(+)-dependent formate dehydrogenase; may protect
cells from exogenous formate; YPL275W and YPL276W comprise
a continuous open reading frame in some S. cerevisiae
strains but not in the genomic reference strain S288C"
/pseudo
/db_xref="GeneID:855853"
/db_xref="SGD:S000006196"
mRNA 18369..19079
/gene="FDH2"
/locus_tag="YPL275W"
/pseudo
/db_xref="GeneID:855853"
/db_xref="SGD:S000006196"
rep_origin 18370..19069
/gene="FDH2"
/locus_tag="YPL275W"
/note="ARS1603; Putative replication origin; identified in
multiple array studies, not yet confirmed by plasmid-based
assay"
/db_xref="SGD:S000130195"
gene <22938..>24701
/gene="SAM3"
/locus_tag="YPL274W"
/db_xref="GeneID:855854"
mRNA <22938..>24701
/gene="SAM3"
/locus_tag="YPL274W"
/product="bifunctional polyamine/amino acid permease SAM3"
/transcript_id="NM_001184088.1"
/db_xref="GeneID:855854"
CDS 22938..24701
/gene="SAM3"
/locus_tag="YPL274W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:17218313]"
/experiment="EXISTENCE:direct assay:GO:0015489 putrescine
transmembrane transporter activity [PMID:17218313]"
/experiment="EXISTENCE:direct assay:GO:0015606 spermidine
transmembrane transporter activity [PMID:17218313]"
/experiment="EXISTENCE:direct assay:GO:0015847 putrescine
transport [PMID:17218313]"
/experiment="EXISTENCE:direct assay:GO:0015848 spermidine
transport [PMID:17218313]"
/experiment="EXISTENCE:mutant phenotype:GO:0000095
S-adenosyl-L-methionine transmembrane transporter activity
[PMID:10497160]"
/experiment="EXISTENCE:mutant phenotype:GO:0005886 plasma
membrane [PMID:10497160]"
/experiment="EXISTENCE:mutant phenotype:GO:0015489
putrescine transmembrane transporter activity
[PMID:17218313]"
/experiment="EXISTENCE:mutant phenotype:GO:0015606
spermidine transmembrane transporter activity
[PMID:17218313]"
/experiment="EXISTENCE:mutant phenotype:GO:0015805
S-adenosyl-L-methionine transport [PMID:10497160]"
/experiment="EXISTENCE:mutant phenotype:GO:0015847
putrescine transport [PMID:17218313]"
/experiment="EXISTENCE:mutant phenotype:GO:0015848
spermidine transport [PMID:17218313]"
/note="High-affinity S-adenosylmethionine permease;
required for utilization of S-adenosylmethionine as a
sulfur source; has similarity to S-methylmethionine
permease Mmp1p"
/codon_start=1
/product="bifunctional polyamine/amino acid permease SAM3"
/protein_id="NP_015049.1"
/db_xref="GeneID:855854"
/db_xref="SGD:S000006195"
/translation="MDILKRGNESDKFTKIETESTTIPNDSDRSGSLIRRMKDSFKQS
NLHVIPEDLENSEQTEQEKIQWKLASQPYQKVLSQRHLTMIAIGGTLGTGLFIGLGYS
LASGPAALLIGFLLVGTSMFCVVQSAAELSCQFPVSGSYATHVSRFIDESVGFTVATN
YALAWLISFPSELIGCALTISYWNQTVNPAVWVAIFYVFIMVLNLFGVRGFAETEFAL
SIIKVIAIFIFIIIGIVLIAGGGPNSTGYIGAKYWHDPGAFAKPVFKNLCNTFVSAAF
SFGGSELVLLTSTESKNISAISRAAKGTFWRIAIFYITTVVIIGCLVPYNDPRLLSGS
NSEDVSASPFVIALSNTGSMGAKVSNFMNVVILVAVVSVCNSCVYASSRLIQALGASG
QLPSVCSYMDRKGRPLVGIGISGAFGLLGFLVASKKEDEVFTWLFALCSISSFFTWFC
ICMSQIRFRMALKAQGRSNDEIAYKSILGVYGGILGCVLNALLIAGEIYVSAAPVGSP
SSAEAFFEYCLSIPIMIVVYFAHRFYRRDWKHFYIKRSEIDLDTGCSVENLELFKAQK
EAEEQLIASKPFYYKIYRFWC"
gene <25087..>26064
/gene="SAM4"
/locus_tag="YPL273W"
/db_xref="GeneID:855855"
mRNA <25087..>26064
/gene="SAM4"
/locus_tag="YPL273W"
/product="S-adenosylmethionine-homocysteine
S-methyltransferase SAM4"
/transcript_id="NM_001184087.1"
/db_xref="GeneID:855855"
CDS 25087..26064
/gene="SAM4"
/locus_tag="YPL273W"
/EC_number="2.1.1.10"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008898
S-adenosylmethionine-homocysteine S-methyltransferase
activity [PMID:11013242]"
/experiment="EXISTENCE:direct assay:GO:0019284
L-methionine salvage from S-adenosylmethionine
[PMID:14189892]"
/experiment="EXISTENCE:direct assay:GO:0033353
S-adenosylmethionine cycle [PMID:14189892]"
/experiment="EXISTENCE:direct assay:GO:0050667
homocysteine metabolic process [PMID:14189892]"
/note="S-adenosylmethionine-homocysteine
methyltransferase; functions along with Mht1p in the
conversion of S-adenosylmethionine (AdoMet) to methionine
to control the methionine/AdoMet ratio; SAM4 has a
paralog, YMR321C, that arose from a single-locus
duplication"
/codon_start=1
/product="S-adenosylmethionine-homocysteine
S-methyltransferase SAM4"
/protein_id="NP_015050.1"
/db_xref="GeneID:855855"
/db_xref="SGD:S000006194"
/translation="MARLPLKQFLADNPKKVLVLDGGQGTELENRGIKVANPVWSTIP
FISESFWSDESSANRKIVKEMFNDFLNAGAEILMTTTYQTSYKSVSENTPIRTLSEYN
NLLNRIVDFSRNCIGEDKYLIGCIGPWGAHICREFTGDYGAEPENIDFYQYFKPQLEN
FNKNDKLDLIGFETIPNIHELKAILSWDESILSRPFYIGLSVHEHGVLRDGTTMEEIA
QVIKDLGDKINPNFSFLGINCVSFNQSPDILESLHQALPNMALLAYPNSGEVYDTEKK
IWLPNSDKLNSWDTVVKQYISSGARIIGGCCRTSPKDIQEISAAVKKYT"
gene complement(<26611..>28164)
/gene="PBI1"
/locus_tag="YPL272C"
/db_xref="GeneID:855856"
mRNA complement(<26611..>28164)
/gene="PBI1"
/locus_tag="YPL272C"
/product="Pbi1p"
/transcript_id="NM_001184086.1"
/db_xref="GeneID:855856"
CDS complement(26611..28164)
/gene="PBI1"
/locus_tag="YPL272C"
/note="hypothetical protein; gene expression induced in
response to ketoconazole; YPL272C is not an essential
gene"
/codon_start=1
/product="Pbi1p"
/protein_id="NP_015051.1"
/db_xref="GeneID:855856"
/db_xref="SGD:S000006193"
/translation="MTTFRPLSSFEKKILTQSLNDQRNGTIFSSTYSKSLSRENDADW
HSDEVTLGTNSSKDDSRLTLPLIATTLKRLIKSQPALFATVNEEWEFEPLKQLKTSDI
VNVIEFETIKDKEVNCHWGVPPPYLLRHAFNKTRFVPGSNKPLWTLYVIDEALLVFHG
HDVLFDIFSAANFHKLFLKELNEISTVTHSEDRILFDVNDINLSELKFPKSIYDSAKL
HLPAMTPQIFHKQTQSFFKSIYYNTLKRPFGYLTNQTSLSSSVSATQLKKYNDILNAH
TSLCGTTVFGIVNNQRFNYLKSIVNQEHICLRSFICGIAMICLKPLVKDFSGTIVFTI
PINLRNHLGLGGSLGLFFKELRVECPLSLIDDELSANEFLTNSNDNEDNDDEFNERLM
EYQFNKVTKHVSGFIMAKLRSWEKNGFNDDDIRRMKYDNDDDFHIQNSRTKLIQINDV
SDISLSMNGDDKSFKIVSTGFTSSINRPTLMSLSYTYCEEMGLNICIHYPDSYNLESF
VECFESFIE"
gene <30079..>30267
/gene="ATP15"
/locus_tag="YPL271W"
/db_xref="GeneID:855857"
mRNA <30079..>30267
/gene="ATP15"
/locus_tag="YPL271W"
/product="F1F0 ATP synthase subunit epsilon"
/transcript_id="NM_001184085.1"
/db_xref="GeneID:855857"
CDS 30079..30267
/gene="ATP15"
/locus_tag="YPL271W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0015986 proton
motive force-driven ATP synthesis [PMID:20691145]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:18722382|PMID:15294286]"
/experiment="EXISTENCE:direct assay:GO:0045259
proton-transporting ATP synthase complex
[PMID:15294286|PMID:17082766]"
/experiment="EXISTENCE:direct assay:GO:0046933
proton-transporting ATP synthase activity, rotational
mechanism [PMID:20691145]"
/experiment="EXISTENCE:mutant phenotype:GO:0015986 proton
motive force-driven ATP synthesis [PMID:8416924]"
/experiment="EXISTENCE:mutant phenotype:GO:0045259
proton-transporting ATP synthase complex [PMID:8416924]"
/experiment="EXISTENCE:mutant phenotype:GO:0046933
proton-transporting ATP synthase activity, rotational
mechanism [PMID:8416924]"
/note="Epsilon subunit of the F1 sector of mitochondrial
F1F0 ATP synthase; which is a large, evolutionarily
conserved enzyme complex required for ATP synthesis; F1
translationally regulates ATP6 and ATP8 expression to
achieve a balanced output of ATP synthase genes encoded in
nucleus and mitochondria; phosphorylated"
/codon_start=1
/product="F1F0 ATP synthase subunit epsilon"
/protein_id="NP_015052.1"
/db_xref="GeneID:855857"
/db_xref="SGD:S000006192"
/translation="MSAWRKAGISYAAYLNVAAQAIRSSLKTELQTASVLNRSQTDAF
YTQYKNGTAASEPTPITK"
gene <30482..>32803
/gene="MDL2"
/locus_tag="YPL270W"
/db_xref="GeneID:855858"
mRNA <30482..>32803
/gene="MDL2"
/locus_tag="YPL270W"
/product="ATP-binding cassette permease MDL2"
/transcript_id="NM_001184084.1"
/db_xref="GeneID:855858"
CDS 30482..32803
/gene="MDL2"
/locus_tag="YPL270W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:11251115]"
/note="Mitochondrial inner membrane half-type ABC
transporter; required for respiratory growth at high
temperature; localizes to vacuole membrane in response to
H2O2; similar to human TAP1 and TAP2 implicated in bare
lymphocyte syndrome and Wegener-like granulomatosis"
/codon_start=1
/product="ATP-binding cassette permease MDL2"
/protein_id="NP_015053.2"
/db_xref="GeneID:855858"
/db_xref="SGD:S000006191"
/translation="MLNGRLPLLRLGICRNMLSRPRLAKLPSIRFRSLVTPSSSQLIP
LSRLCLRSPAVGKSLILQSFRCNSSKTVPETSLPSASPISKGSARSAHAKEQSKTDDY
KDIIRLFMLAKRDWKLLLTAILLLTISCSIGMSIPKVIGIVLDTLKTSSGSDFFDLKI
PIFSLPLYEFLSFFTVALLIGCAANFGRFILLRILSERVVARLRANVIKKTLHQDAEF
FDNHKVGDLISRLGSDAYVVSRSMTQKVSDGVKALICGVVGVGMMCSLSPQLSILLLF
FTPPVLFSASVFGKQIRNTSKDLQEATGQLTRVAEEQLSGIKTVQSFVAEGNELSRYN
VAIRDIFQVGKTAAFTNAKFFTTTSLLGDLSFLTVLAYGSYLVLQSQLSIGDLTAFML
YTEYTGNAVFGLSTFYSEIMQGAGAASRLFELTDRKPSISPTVGHKYKPDRGVIEFKD
VSFSYPTRPSVQIFKNLNFKIAPGSSVCIVGPSGRGKSTIALLLLRYYNPTTGTITID
NQDISKLNCKSLRRHIGIVQQEPVLMSGTIRDNITYGLTYTPTKEEIRSVAKQCFCHN
FITKFPNTYDTVIGPHGTLLSGGQKQRIAIARALIKKPTILILDEATSALDVESEGAI
NYTFGQLMKSKSMTIVSIAHRLSTIRRSENVIVLGHDGSVVEMGKFKELYANPTSALS
QLLNEKAAPGPSDQQLQIEKVIEKEDLNESKEHDDQKKDDNDDNDNNHDNDSNNQSPE
TKDNNSDDIEKSVEHLLKDAAKEANPIKITPQP"
gene <33013..>34947
/gene="KAR9"
/locus_tag="YPL269W"
/db_xref="GeneID:855859"
mRNA <33013..>34947
/gene="KAR9"
/locus_tag="YPL269W"
/product="Kar9p"
/transcript_id="NM_001184083.1"
/db_xref="GeneID:855859"
CDS 33013..34947
/gene="KAR9"
/locus_tag="YPL269W"
/experiment="EXISTENCE:direct assay:GO:0000776 kinetochore
[PMID:26906737]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:26906737]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:26906737]"
/experiment="EXISTENCE:direct assay:GO:0005816 spindle
pole body [PMID:20534809]"
/experiment="EXISTENCE:direct assay:GO:0005881 cytoplasmic
microtubule [PMID:26906737]"
/experiment="EXISTENCE:direct assay:GO:0005938 cell cortex
[PMID:9442113]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:9442113]"
/experiment="EXISTENCE:genetic interaction:GO:0031578
mitotic spindle orientation checkpoint signaling
[PMID:24850890]"
/experiment="EXISTENCE:mutant phenotype:GO:0030473 nuclear
migration along microtubule [PMID:9442113]"
/experiment="EXISTENCE:mutant phenotype:GO:0051293
establishment of spindle localization [PMID:22385961]"
/note="Spindle positioning factor; orients astral
microtubules, connecting them to actin cables at the
cortex with Bim1p and Myo2, resulting in proper spindle
positioning; targeted for StuBL-dependent degradation at
kinetochores by Slx5p-Slx8p, ensuring chromosome
transmission fidelity and correct spindle positioning;
role in karyogamy; localizes to the shmoo tip, the growing
bud-tip, the nucleus, the kinetochore, the spindle and
microtubules; homolog of adenomatous polyposis coli"
/codon_start=1
/product="Kar9p"
/protein_id="NP_015054.1"
/db_xref="GeneID:855859"
/db_xref="SGD:S000006190"
/translation="MDNDGPRSMTIGDDFQENFCERLERIHNTLHSINDCNSLNESTT
SISETLLVQFYDDLENVASVIPDLVNKKRLGKDDILLFMDWLLLKKYMLYQFISDVHN
IEEGFAHLLDLLEDEFSKDDQDSDKYNRFSPMFDVIEESTQIKTQLEPWLTNLKELLD
TSLEFNEISKDHMDTLHKIINSNISYCLEIQEERFASPIRHTPSFTLEQLVKLLGTHT
ETTEPKVPKFSPAEDILSRKFLNLKKNIPPIEKSLTDILPQRIVQFGHRNITNITTLQ
TILQKKYELIMKDYRFMNSEFRELKVELIDKRWNILFINLNHELLYILDEIERLQSKL
LTTKYTKDITIRLERQLERKSKTVSKTFNIIYRALEFSLLDAGVASKTNELAQRWLNI
KPTADKILIKSSASNKIATSKKKIPKPKSLGFGRPNSVIGTITQDFQERVAINEGDSN
KTPENSTTVALKGKKLGKALLQKMNIKPATSPNSSNAINPFFDPESPNKGKLILSSVP
PLPYDETDETTLRVSRGENEKSPDSFITSRHENKVQITETPLMAKNKSVLDIEKDKWN
HYRSLPSRIPIYKDKVVKVTVENTPIAKVFQTPPTKITTPNSQVWVPSTRRRTRLRPP
TPLSQLLSPREGRLDKTPTY"
gene <35236..>37845
/gene="PLC1"
/locus_tag="YPL268W"
/db_xref="GeneID:855860"
mRNA <35236..>37845
/gene="PLC1"
/locus_tag="YPL268W"
/product="phosphatidylinositol phospholipase C"
/transcript_id="NM_001184082.1"
/db_xref="GeneID:855860"
CDS 35236..37845
/gene="PLC1"
/locus_tag="YPL268W"
/EC_number="3.1.4.11"
/experiment="EXISTENCE:direct assay:GO:0000775 chromosome,
centromeric region [PMID:12756538|PMID:10779349]"
/experiment="EXISTENCE:direct assay:GO:0004435
phosphatidylinositol-4,5-bisphosphate phospholipase C
activity [PMID:8395015|PMID:15485855]"
/experiment="EXISTENCE:direct assay:GO:0009395
phospholipid catabolic process [PMID:8395015]"
/experiment="EXISTENCE:genetic interaction:GO:0001402
signal transduction involved in filamentous growth
[PMID:10514491]"
/experiment="EXISTENCE:mutant phenotype:GO:0004435
phosphatidylinositol-4,5-bisphosphate phospholipase C
activity [PMID:7753023|PMID:9784626]"
/experiment="EXISTENCE:mutant phenotype:GO:0009395
phospholipid catabolic process [PMID:7753023]"
/experiment="EXISTENCE:mutant phenotype:GO:0032958
inositol phosphate biosynthetic process [PMID:10390371]"
/experiment="EXISTENCE:mutant phenotype:GO:0034501 protein
localization to kinetochore [PMID:19205744]"
/experiment="EXISTENCE:physical interaction:GO:0000776
kinetochore [PMID:10779349]"
/experiment="EXISTENCE:physical interaction:GO:0001402
signal transduction involved in filamentous growth
[PMID:10514491]"
/note="Phospholipase C; hydrolyzes phosphatidylinositol
4,5-biphosphate (PIP2) to generate the signaling molecules
inositol 1,4,5-triphosphate (IP3) and 1,2-diacylglycerol
(DAG); involved in regulating many cellular processes;
Plc1p and inositol polyphosphates are required for
acetyl-CoA homeostasis which regulates global histone
acetylation"
/codon_start=1
/product="phosphatidylinositol phospholipase C"
/protein_id="NP_015055.1"
/db_xref="GeneID:855860"
/db_xref="SGD:S000006189"
/translation="MTESAIDDQRFNLTKELQRHSCRDQGKITQKDDALDFISYSSFQ
SSFNTDQKSANNGSTVRRSIRSIFRRAAELPRVHMGPLTYSHGINELVNKKLRKDCDL
STLCRVLQRGIRMIRMTRRRRKFYEFKLINNNGQIIWKDGSKYLELDSVKDIRIGDTA
STYQEEVDPKRLRSDSKLWIAIIYKVSNKLKALHVVALNELDFNTFLSCICGLVKLRR
ELMESILLPDNSQFARIHWQITVSEKEEDEKKDTLSFADVKKLCDKFHIYVSTGQLLE
FFQLADINHNGLLNYFEFEKFIKILKNRKEVNMIWSKFTKPPHSHLSFENFFQFLITE
QHEQVDRQTAWSYFIKYREPTQLTMGQDGFTKFLKEQPYLVEVKEELYSKPLNHYFIA
SSHNTYLLGKQIAETPSVEGYIQVLQQGCRCVEIDIWDGENGPVVCHGFLTSAIPLKT
VIRVIKKYAFITSPYPLIISLEINCNKDNQKLASLIMREVLAEQLYFVGTRTDKLPSP
RELKHKILLKSKKTSEATRGLSVNEPFPSSFSSSYESANEQELRMKDDSTNSSSATNS
SSMQRIKRIGLKKHADIINDVSNISGIHGIKFRNFSLPESKTIAHCFSLNERKVEYMI
KDKHLKLSLDKHNRRYLMRVYPHVLRYKSSNFNPIPFWKAGVQMVATNWQTNDIGQQL
NLAMFQILDHQPDGSFKSGYVLKPKKLLPVVTKAKMIPLIYEHFENGSDPVTVKIRIL
STQLLPRLNDTSPSRNNTNSFVKVEFHTDDEPTMPISIDKGTRISATEASTKSSQGNG
FNPIWDAEVSITLKDTDLTFIKFMVISEETQIASVCLKLNYLRMGYRHIPLFNMEGEQ
YIFCTLFIHTQIL"
gene <38169..>38798
/gene="ACM1"
/locus_tag="YPL267W"
/db_xref="GeneID:855861"
mRNA <38169..>38798
/gene="ACM1"
/locus_tag="YPL267W"
/product="Acm1p"
/transcript_id="NM_001184081.1"
/db_xref="GeneID:855861"
CDS 38169..38798
/gene="ACM1"
/locus_tag="YPL267W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:18498748]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:18498748]"
/experiment="EXISTENCE:direct assay:GO:0031397 negative
regulation of protein ubiquitination
[PMID:18519589|PMID:18596038]"
/experiment="EXISTENCE:direct assay:GO:0055105
ubiquitin-protein transferase inhibitor activity
[PMID:18498748|PMID:18519589|PMID:18596038]"
/experiment="EXISTENCE:genetic interaction:GO:0055105
ubiquitin-protein transferase inhibitor activity
[PMID:18498748]"
/experiment="EXISTENCE:mutant phenotype:GO:0055105
ubiquitin-protein transferase inhibitor activity
[PMID:18498748|PMID:18596038]"
/note="Pseudosubstrate inhibitor of the APC/C; suppresses
APC/C [Cdh1]-mediated proteolysis of mitotic cyclins;
associates with Cdh1p, Bmh1p and Bmh2p; cell cycle
regulated protein; the anaphase-promoting
complex/cyclosome is also known as APC/C"
/codon_start=1
/product="Acm1p"
/protein_id="NP_015056.1"
/db_xref="GeneID:855861"
/db_xref="SGD:S000006188"
/translation="MISPSKKRTILSSKNINQKPRAVVKGNELRSPSKRRSQIDTDYA
LRRSPIKTIQISKAAQFMLYEETAEERNIAVHRHNEIYNNNNSVSNENNPSQVKENLS
PAKICPYERAFLREGGRIALKDLSVDEFKGYIQDPLTDETIPLTLPLGDKKISLPSFI
TPPRNSKISIFFTSKHQGQNPETKISRSTDDVSEKKVVRKLSFHVYEDE"
gene <39121..>40077
/gene="DIM1"
/locus_tag="YPL266W"
/gene_synonym="CDH1"
/db_xref="GeneID:855862"
mRNA <39121..>40077
/gene="DIM1"
/locus_tag="YPL266W"
/gene_synonym="CDH1"
/product="putative dimethyladenosine transferase"
/transcript_id="NM_001184080.1"
/db_xref="GeneID:855862"
CDS 39121..40077
/gene="DIM1"
/locus_tag="YPL266W"
/gene_synonym="CDH1"
/EC_number="2.1.1.183"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/experiment="EXISTENCE:mutant phenotype:GO:0000154 rRNA
modification [PMID:8064863]"
/experiment="EXISTENCE:mutant phenotype:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:8064863]"
/note="Essential 18S rRNA dimethylase (dimethyladenosine
transferase); responsible for conserved m6(2)Am6(2)A
dimethylation in 3'-terminal loop of 18S rRNA, part of 90S
and 40S pre-particles in nucleolus, involved in
pre-ribosomal RNA processing; human homolog DIMT1
complements yeast dim1 mutant"
/codon_start=1
/product="putative dimethyladenosine transferase"
/protein_id="NP_015057.1"
/db_xref="GeneID:855862"
/db_xref="SGD:S000006187"
/translation="MGKAAKKKYSGATSSKQVSAEKHLSSVFKFNTDLGQHILKNPLV
AQGIVDKAQIRPSDVVLEVGPGTGNLTVRILEQAKNVVAVEMDPRMAAELTKRVRGTP
VEKKLEIMLGDFMKTELPYFDICISNTPYQISSPLVFKLINQPRPPRVSILMFQREFA
LRLLARPGDSLYCRLSANVQMWANVTHIMKVGKNNFRPPPQVESSVVRLEIKNPRPQV
DYNEWDGLLRIVFVRKNRTISAGFKSTTVMDILEKNYKTFLAMNNEMVDDTKGSMHDV
VKEKIDTVLKETDLGDKRAGKCDQNDFLRLLYAFHQVGIHFS"
gene <41043..>42869
/gene="DIP5"
/locus_tag="YPL265W"
/db_xref="GeneID:855863"
mRNA <41043..>42869
/gene="DIP5"
/locus_tag="YPL265W"
/product="dicarboxylic amino acid permease"
/transcript_id="NM_001184079.1"
/db_xref="GeneID:855863"
CDS 41043..42869
/gene="DIP5"
/locus_tag="YPL265W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005310
dicarboxylic acid transmembrane transporter activity
[PMID:10654085]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0006865 amino acid
transport [PMID:10654085]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="Dicarboxylic amino acid permease; mediates
high-affinity and high-capacity transport of L-glutamate
and L-aspartate; also a transporter for Gln, Asn, Ser,
Ala, and Gly; relocalizes from plasma membrane to vacuole
upon DNA replication stress"
/codon_start=1
/product="dicarboxylic amino acid permease"
/protein_id="NP_015058.1"
/db_xref="GeneID:855863"
/db_xref="SGD:S000006186"
/translation="MKMPLKKMFTSTSPRNSSSLDSDHDAYYSKQNPDNFPVKEQEIY
NIDLEENNVSSRSSTSTSPSARDDSFAVPDGKDENTRLRKDLKARHISMIAIGGSLGT
GLLIGTGTALLTGGPVAMLIAYAFVGLLVFYTMACLGEMASYIPLDGFTSYASRYVDP
ALGFAIGYTYLFKYFILPPNQLTAAALVIQYWISRDRVNPGVWITIFLVVIVAINVVG
VKFFGEFEFWLSSFKVMVMLGLILLLFIIMLGGGPNHDRLGFRYWRDPGAFKEYSTAI
TGGKGKFVSFVAVFVYSLFSYTGIELTGIVCSEAENPRKSVPKAIKLTVYRIIVFYLC
TVFLLGMCVAYNDPRLLSTKGKSMSAAASPFVVAIQNSGIEVLPHIFNACVLVFVFSA
CNSDLYVSSRNLYALAIDGKAPKIFAKTSRWGVPYNALILSVLFCGLAYMNVSSGSAK
IFNYFVNVVSMFGILSWITILIVYIYFDKACRAQGIDKSKFAYVAPGQRYGAYFALFF
CILIALIKNFTVFLGHKFDYKTFITGYIGLPVYIISWAGYKLIYKTKVIKSTDVDLYT
FKEIYDREEEEGRMKDQEKEERLKSNGKNMEWFYEKFLGNIF"
rep_origin 42977..43212
/note="ARS1604; Autonomously Replicating Sequence"
/db_xref="SGD:S000118411"
gene complement(<43283..>44344)
/locus_tag="YPL264C"
/db_xref="GeneID:855864"
mRNA complement(<43283..>44344)
/locus_tag="YPL264C"
/product="uncharacterized protein"
/transcript_id="NM_001184078.1"
/db_xref="GeneID:855864"
CDS complement(43283..44344)
/locus_tag="YPL264C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:31945465]"
/note="Endoplasmic reticulum hypothetical protein;
physically interacts with Hsp82p; YPL264C is not an
essential gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015059.1"
/db_xref="GeneID:855864"
/db_xref="SGD:S000006185"
/translation="MTLQRISKDYLKPNYGLILLIVSYFFNSSMVVSTKVLENDPLET
SQSRINPLQILLVRMSITYCCTLVYMHWNKQSVPDIPWGPAPCRKWLILRGIMGFFGV
FGMYFSLMYLSISDAVLITFMSPTLTIFLSFLLLGEPFSKLEALGSLISFSGVVLIIR
PTFLFGEQTQGQQSPQDDIVETQNPKLRLIAIGVSLLGVCGLSSVYIIIRYIGNKAHA
IMSVSYFSLVTTVVAALGVLLIPSMSLQLPHSWKQWGLFLNLGISGFIHQILLTMGIQ
RERAGRGSLMTYTQVIYAVFWDVVLFHHWPNIWTWCGMAVIVSSTIWVINMRASKQNV
VATAELLSTSDFELDDLED"
gene complement(<44551..>46506)
/gene="KEL3"
/locus_tag="YPL263C"
/db_xref="GeneID:855865"
mRNA complement(<44551..>46506)
/gene="KEL3"
/locus_tag="YPL263C"
/product="Kel3p"
/transcript_id="NM_001184077.1"
/db_xref="GeneID:855865"
CDS complement(44551..46506)
/gene="KEL3"
/locus_tag="YPL263C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="Cytoplasmic hypothetical protein"
/codon_start=1
/product="Kel3p"
/protein_id="NP_015060.1"
/db_xref="GeneID:855865"
/db_xref="SGD:S000006184"
/translation="MAKKNKKDKEAKKARAELKNQKNQKKQEKKFQKNKNKSLNGEED
DESDQDLDEILSSFSKKQIELEHVDITSVEKPSCRTHPLMFANPQHNKHELFIFGGEF
TDPETKLTHFYNDLYSYSIKNNSWKKYVSQNAPLPRSSAAVAVHPSGIALLHGGEFSS
PKQSKFYHYSDTWLFDCVERKFTKLEFGGRDSSPSARSGHRIIAWKNYFILFGGFRDL
GNGQTSYLNDLWCFDISTYKWTKLETNSKPDARSGHCFIPTDNSAILMGGYCKIIAKN
NKNLMKGKILNDAWKLNLTPDPKKWQWEKLKNFKNQPSPRVGYSFNLWKQNKSVAFGG
VYDLQETEESLESVFYNDLYMFHLELNKWSKLRIKPQRQTNSKNSPATSKRKSNKDQE
KELQDLLNSILAKSNLNDDDDDNDDNSTTGPNSIDDDEDNEDDSDLDNQEDITISNQL
PHPRFNAATCVVGDSLFIYSGVWELGEKDYPINSFYSIDLNKLDGVKVYWEDLSAIEE
AKRLGDRDSDEDEFEYEDDEEDEDDGEEEQDAGPLEGDEDEESESDDDKQAQMEIPDE
RSWLPHPKPFETLRAFYLREGANFLTWSISNNRNLKGKQLKTKSFELCEDRWWERRDQ
VTLEEERLEDTGGIIERDTTTKPSKRR"
gene <47336..>48802
/gene="FUM1"
/locus_tag="YPL262W"
/db_xref="GeneID:855866"
mRNA <47336..>48802
/gene="FUM1"
/locus_tag="YPL262W"
/product="fumarase FUM1"
/transcript_id="NM_001184076.1"
/db_xref="GeneID:855866"
CDS 47336..48802
/gene="FUM1"
/locus_tag="YPL262W"
/EC_number="4.2.1.2"
/experiment="EXISTENCE:direct assay:GO:0004333 fumarate
hydratase activity [PMID:3040736]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:20231875]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:11502169|PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:11585823]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:11585823]"
/experiment="EXISTENCE:mutant phenotype:GO:0006099
tricarboxylic acid cycle [PMID:1587456]"
/experiment="EXISTENCE:mutant phenotype:GO:0006106
fumarate metabolic process [PMID:1587456]"
/note="Fumarase; converts fumaric acid to L-malic acid in
the TCA cycle; cytosolic and mitochondrial distribution
determined by the N-terminal targeting sequence, protein
conformation, and status of glyoxylate shunt;
phosphorylated in mitochondria"
/codon_start=1
/product="fumarase FUM1"
/protein_id="NP_015061.1"
/db_xref="GeneID:855866"
/db_xref="SGD:S000006183"
/translation="MLRFTNCSCKTFVKSSYKLNIRRMNSSFRTETDAFGEIHVPADK
YWGAQTQRSFQNFKIGGARERMPLPLVHAFGVLKKSAAIVNESLGGLDPKISKAIQQA
ADEVASGKLDDHFPLVVFQTGSGTQSNMNANEVISNRAIEILGGKIGSKQVHPNNHCN
QSQSSNDTFPTVMHIAASLQIQNELIPELTNLKNALEAKSKEFDHIVKIGRTHLQDAT
PLTLGQEFSGYVQQVENGIQRVAHSLKTLSFLAQGGTAVGTGLNTKPGFDVKIAEQIS
KETGLKFQTAPNKFEALAAHDAIVECSGALNTLACSLFKIAQDIRYLGSGPRCGYHEL
MLPENEPGSSIMPGKVNPTQNEALTQVCVQVMGNNAAITFAGSQGQFELNVFKPVMIA
NLLNSIRLITDAAYSFRVHCVEGIKANEPRIHELLTKSLMLVTALNPKIGYDAASKVA
KNAHKKGITLKESALELGVLTEKEFDEWVVPEHMLGPK"
gene complement(<48996..>49304)
/locus_tag="YPL261C"
/db_xref="GeneID:855868"
mRNA complement(<48996..>49304)
/locus_tag="YPL261C"
/product="uncharacterized protein"
/transcript_id="NM_001348883.1"
/db_xref="GeneID:855868"
CDS complement(48996..49304)
/locus_tag="YPL261C"
/note="hypothetical protein; conserved among S. cerevisiae
strains; YPL261C is not an essential gene; partially
overlaps verified ORF YPL260W"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335821.1"
/db_xref="GeneID:855868"
/db_xref="SGD:S000006182"
/translation="MLFLDSYSLLIQFQRFKNWESPRRFSSSFPLLLFVFKPIFAAKL
LKEICSSGVLSYSLSFLKIPLSVMRVILGPLPDDKKLKNDAKYSFMNYFIITCIGIIM
"
gene <49303..>50958
/gene="CUB1"
/locus_tag="YPL260W"
/db_xref="GeneID:855867"
mRNA <49303..>50958
/gene="CUB1"
/locus_tag="YPL260W"
/product="Cub1p"
/transcript_id="NM_001184074.1"
/db_xref="GeneID:855867"
CDS 49303..50958
/gene="CUB1"
/locus_tag="YPL260W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="Conserved fungal gene linked to DNA repair and
proteasome function; putative substrate of cAMP-dependent
protein kinase (PKA); green fluorescent protein
(GFP)-fusion protein localizes to the cytoplasm and
nucleus; YPL260W is not an essential gene; protein
abundance increases in response to DNA replication stress"
/codon_start=1
/product="Cub1p"
/protein_id="NP_015063.1"
/db_xref="GeneID:855867"
/db_xref="SGD:S000006181"
/translation="MFASAGQQHPQIVPKEEESILNYLLEVRSSLAKLKQNRTQYLNS
KDVQTTYQHVLTKVRELDDIRKNSHETPAKSAATLIHSTELHNRVDSVLDDVFQLLSL
CFLTVGLKNSAPATYASLSTVESLLEHLNESNVFTHHDLSPIKERLEEISKIVEQKNS
SPAYDEDGNDDRLREIDNERKKNKIEEDLLLRAKLKHCKDEYDILEGKLEEIDPSLST
VMEKLFRIRRGLLSLVASAKKTMSKSDINTNSLLQEQNDLQTNNESLTDDKHLVSQEY
VHEKLSVLKNELSELESNRDDSGKFKSLESHQVAEKGQSVLNGLLDDCHDLVNDLSHQ
KNGGLTLDPYLQPIYEQLIDIKTTLENLMITRRWTLRETDLFSYQKKLNEIDNKRING
KFPTKSQDSKGQSILLYLLRRCYAIIYKLLESSEPVSEALQPIHNQLSTVRRCLLELK
RMGGVNNERELYPYQMKLASLDNLRTEGIFYDSDGNIPEGQGILNALLAECFDILHEL
KVEAEEKAQNSTSSDGSDDDDNGESGIDSNSNDSEPESEYQQE"
gene complement(<51244..>52671)
/gene="APM1"
/locus_tag="YPL259C"
/gene_synonym="YAP54"
/db_xref="GeneID:855869"
mRNA complement(<51244..>52671)
/gene="APM1"
/locus_tag="YPL259C"
/gene_synonym="YAP54"
/product="Apm1p"
/transcript_id="NM_001184073.1"
/db_xref="GeneID:855869"
CDS complement(51244..52671)
/gene="APM1"
/locus_tag="YPL259C"
/gene_synonym="YAP54"
/experiment="EXISTENCE:direct assay:GO:0030121 AP-1
adaptor complex [PMID:10564262]"
/experiment="EXISTENCE:mutant phenotype:GO:0006896 Golgi
to vacuole transport [PMID:17003107]"
/note="One of two alternative Mu1-like medium subunits of
the clathrin-associated AP-1 complex; binds clathrin;
involved in clathrin-dependent Golgi protein sorting"
/codon_start=1
/product="Apm1p"
/protein_id="NP_015064.1"
/db_xref="GeneID:855869"
/db_xref="SGD:S000006180"
/translation="MASAVYFCDHNGKPLLSRRYRDDIPLSAIDKFPILLSDLEEQSN
LIPPCLNHNGLEYLFIQHNDLYVVAIVTSLSANAAAIFTFLHKLVEVLSDYLKTVEEE
SIRDNFVIIYELLDEVMDYGIPQITETKMLKQYITQKSFKLVKSAKKKRNATRPPVAL
TNSVSWRPEGITHKKNEAFLDIVESINMLMTQKGQVLRSEIIGDVKVNSKLSGMPDLK
LGINDKGIFSKYLDDDTNIPSASATTSDNNTETDKKPSITSSSATNKKKVNIELEDLK
FHQCVRLSKFENEKIITFIPPDGKFDLMNYRLSTTIKPLIWCDVNVQVHSNSRIEIHC
KAKAQIKRKSTATNVEILIPVPDDADTPTFKYSHGSLKYVPEKSAILWKIRSFPGGKE
YSMSAELGLPSISNNEDGNRTMPKSNAEILKGPVQIKFQIPYFTTSGIQVRYLKINEP
KLQYKSYPWVRYITQSGDDYTIRLT"
gene 52977..53465
/gene="SUT390"
/locus_tag="YNCP0025W"
/db_xref="GeneID:84313897"
ncRNA 52977..53465
/ncRNA_class="other"
/gene="SUT390"
/locus_tag="YNCP0025W"
/product="SUT390"
/note="Stable Unannotated Transcript functional ncRNA;
improves endogenous invertase secretion when deleted"
/transcript_id="NR_188208.1"
/db_xref="GeneID:84313897"
/db_xref="SGD:S000345043"
gene complement(<53498..>55153)
/gene="THI21"
/locus_tag="YPL258C"
/db_xref="GeneID:855870"
mRNA complement(<53498..>55153)
/gene="THI21"
/locus_tag="YPL258C"
/product="bifunctional hydroxymethylpyrimidine
kinase/phosphomethylpyrimidine kinase"
/transcript_id="NM_001184072.1"
/db_xref="GeneID:855870"
CDS complement(53498..55153)
/gene="THI21"
/locus_tag="YPL258C"
/EC_number="2.7.1.49"
/EC_number="2.7.4.7"
/experiment="EXISTENCE:direct assay:GO:0008902
hydroxymethylpyrimidine kinase activity [PMID:15614489]"
/experiment="EXISTENCE:direct assay:GO:0008972
phosphomethylpyrimidine kinase activity [PMID:15614489]"
/experiment="EXISTENCE:genetic interaction:GO:0008902
hydroxymethylpyrimidine kinase activity [PMID:15614489]"
/experiment="EXISTENCE:genetic interaction:GO:0008972
phosphomethylpyrimidine kinase activity
[PMID:15614489|PMID:10383756]"
/experiment="EXISTENCE:genetic interaction:GO:0009228
thiamine biosynthetic process [PMID:10383756]"
/note="Hydroxymethylpyrimidine (HMP) and HMP-phosphate
kinase; involved in thiamine biosynthesis; member of a
gene family with THI20 and THI22; functionally redundant
with Thi20p"
/codon_start=1
/product="bifunctional hydroxymethylpyrimidine
kinase/phosphomethylpyrimidine kinase"
/protein_id="NP_015065.1"
/db_xref="GeneID:855870"
/db_xref="SGD:S000006179"
/translation="MTYSTVNINTPPPYLALASNEKLPTVLSIAGTDPSGGAGVEADV
KTITAHRCYAMTCITALNAQTPVKVYSINNTPKEVVSQILDANLQDMKCDVIKTGMLT
TAAIEVLHEKLLQLGENRPKLVVDPVLVATSGSSLAGKDIASLITEKIAPFADILTPN
IPECFKLLGEDREISKLRDIFEVAKDLAKITKCSNILVKGGHIPWNDEEGKYITDVLY
LGAEQRFITFKGNFVNTTHTHGTGCTLASAIASNLARGYSLPQSVYGGIEYVQNAVAI
GCDVTKETVKDNGPINHVYAIEIPLEKMLSDECFTASDAVHKKPVKSSLNKIPGGSFY
KYLINHPKVKPHWDSYVNHDFVRKVADGSLEPKKFQFFIEQDYLYLVNYARISCIAGS
KSPCLEDLEKELVIVECVRNGLCQHERRLREEFGIKDPDYLQKIQRGPALRAYCRYFN
DVSRRGNWQELVIALNPCLMGYVHALTKIKDEVTAAEGSVYREWCETYSSSWCHEAML
EGEKLLNHILETYPPEKLDTLVTIYAEVCELEANFWTAALEYE"
repeat_region complement(55657..55858)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007184"
gene complement(56169..56274)
/locus_tag="YNCP0001C"
/db_xref="GeneID:855815"
tRNA complement(join(56169..56204,56239..56274))
/locus_tag="YNCP0001C"
/product="tRNA-Trp"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Tryptophan tRNA (tRNA-Trp), predicted by
tRNAscan-SE analysis"
/db_xref="GeneID:855815"
/db_xref="SGD:S000006776"
repeat_region 56290..56358
/note="Ty3 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007198"
repeat_region 56409..56451
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007189"
mobile_element 56452..62375
/note="YPLWTy1-1; Ty1 element, LTR retrotransposon of the
Copia (Pseudoviridae) group; contains co-transcribed genes
TYA Gag and TYB Pol, encoding proteins involved in
structure and function of virus-like particles, flanked by
two direct repeats"
/mobile_element_type="retrotransposon:YPLWTy1-1"
/db_xref="SGD:S000007199"
repeat_region 56452..56788
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007190"
gene <56748..>62016
/locus_tag="YPL257W-B"
/db_xref="GeneID:855817"
mRNA <56748..>62016
/locus_tag="YPL257W-B"
/product="gag-pol fusion protein"
/transcript_id="NM_001184392.2"
/db_xref="GeneID:855817"
CDS join(56748..58052,58054..62016)
/locus_tag="YPL257W-B"
/EC_number="2.7.7.7"
/EC_number="2.7.7.49"
/EC_number="3.1.26.4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9448009]"
/ribosomal_slippage
/note="Retrotransposon TYA Gag and TYB Pol genes;
transcribed/translated as one unit; polyprotein is
processed to make a nucleocapsid-like protein (Gag),
reverse transcriptase (RT), protease (PR), and integrase
(IN); similar to retroviral genes"
/codon_start=1
/product="gag-pol fusion protein"
/protein_id="NP_058189.1"
/db_xref="GeneID:855817"
/db_xref="SGD:S000007358"
/translation="MESQQLSQHSPISHGSACASVTSKEVHTNQDPLDVSASKTEECE
KASTKANSQQTTTPASSAVPENPHHASPQPASVPPPQNGPYPQQCMMTQNQANPSGWS
FYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVRQITDDELT
FLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQ
YNGSTPADAFETKVTNIIDRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNM
TVAELFLDIHAIYEEQQGSRNSKPNYRRNLSDEKNDSRSYTNTTKPKVIARNPQKTNN
SKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLGQELTESTVNHTNHS
DDELPGHLLLDSGASRTLIRSAHHIHSASSNPDINVVDAQKRNIPINAIGDLQFHFQD
NTKTSIKVLHTPNIAYDLLSLNELAAVDITACFTKNVLERSDGTVLAPIVKYGDFYWV
SKKYLLPSNISVPTINNVHTSESTRKYPYPFIHRMLAHANAQTIRYSLKNNTITYFNE
SDVDWSSAIDYQCPDCLIGKSTKHRHIKGSRLKYQNSYEPFQYLHTDIFGPVHNLPNS
APSYFISFTDETTKFRWVYPLHDRREDSILDVFTTILAFIKNQFQASVLVIQMDRGSE
YTNRTLHKFLEKNGITPCYTTTADSRAHGVAERLNRTLLDDCRTQLQCSGLPNHLWFS
AIEFSTIVRNSLASPKSKKSARQHAGLAGLDISTLLPFGQPVIVNDHNPNSKIHPRGI
PGYALHPSRNSYGYIIYLPSLKKTVDTTNYVILQGKESRLDQFNYDALTFDEDLNRLT
ASYHSFIASNEIQESNDLNIESDHDFQSDIELHPEQPRNVLSKAVSPTDSTPPSTHTE
DSKRVSKTNIRAPREVDPNISESNILPSKKRSSTPQISNIESTGSGGMHKLNVPLLAP
MSQSNTHESSHASKSKDFRHSDSYSENETNHTNVPISSTGGTNNKTVPQISDQETEKR
IIHRSPSIDASPPENNSSHNIVPIKTPTTVSEQNTEESIIADLPLPDLPPESPTEFPD
PFKELPPINSRQTNSSLGGIGDSNAYTTINSKKRSLEDNETEIKVSRDTWNTKNMRSL
EPPRSKKRIHLIAAVKAVKSIKPIRTTLRYDEAITYNKDIKEKEKYIEAYHKEVNQLL
KMKTWDTDEYYDRKEIDPKRVINSMFIFNKKRDGTHKARFVARGDIQHPDTYDSGMQS
NTVHHYALMTSLSLALDNNYYITQLDISSAYLYADIKEELYIRPPPHLGMNDKLIRLK
KSLYGLKQSGANWYETIKSYLIKQCGMEEVRGWSCVFKNSQVTICLFVDDMILFSKDL
NANKKIITTLKKQYDTKIINLGESDNEIQYDILGLEIKYQRGKYMKLGMENSLTEKIP
KLNVPLNPKGRKLSAPGQPGLYIDQDELEIDEDEYKEKVHEMQKLIGLASYVGYKFRF
DLLYYINTLAQHILFPSRQVLDMTYELIQFMWDTRDKQLIWHKNKPTEPDNKLVAISD
ASYGNQPYYKSQIGNIYLLNGKVIGGKSTKASLTCTSTTEAEIHAISESVPLLNNLSY
LIQELNKKPIIKGLLTDSRSTISIIKSTNEEKFRNRFFGTKAMRLRDEVSGNNLYVYY
IETKKNIADVMTKPLPIKTFKLLTNKWIH"
gene <56748..>58070
/locus_tag="YPL257W-A"
/db_xref="GeneID:855816"
mRNA <56748..>58070
/locus_tag="YPL257W-A"
/product="gag protein"
/transcript_id="NM_001184391.1"
/db_xref="GeneID:855816"
CDS 56748..58070
/locus_tag="YPL257W-A"
/note="Retrotransposon TYA Gag gene co-transcribed with
TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is
a nucleocapsid protein that is the structural constituent
of virus-like particles (VLPs); similar to retroviral Gag"
/codon_start=1
/product="gag protein"
/protein_id="NP_058190.1"
/db_xref="GeneID:855816"
/db_xref="SGD:S000007357"
/translation="MESQQLSQHSPISHGSACASVTSKEVHTNQDPLDVSASKTEECE
KASTKANSQQTTTPASSAVPENPHHASPQPASVPPPQNGPYPQQCMMTQNQANPSGWS
FYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVRQITDDELT
FLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQ
YNGSTPADAFETKVTNIIDRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNM
TVAELFLDIHAIYEEQQGSRNSKPNYRRNLSDEKNDSRSYTNTTKPKVIARNPQKTNN
SKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLRPGTY"
repeat_region 62039..62375
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007191"
repeat_region 62389..62720
/note="Ty2 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007192"
repeat_region 62724..63006
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007193"
gene <63279..>63860
/locus_tag="YPL257W"
/db_xref="GeneID:855818"
mRNA <63279..>63860
/locus_tag="YPL257W"
/product="uncharacterized protein"
/transcript_id="NM_001184071.1"
/db_xref="GeneID:855818"
CDS 63279..63860
/locus_tag="YPL257W"
/note="hypothetical protein; homozygous diploid deletion
strain exhibits low budding index; physically interacts
with Hsp82p; YPL257W is not an essential gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015066.1"
/db_xref="GeneID:855818"
/db_xref="SGD:S000006178"
/translation="MTAHANERISDVSPSARGSSSESKYDKLCRVLFFIAITKSSFTP
EHILYKHSIFTDKPILADIVTFMYAAFVSIGWFLIWGERAYRTQEMGQPPMYSNINYH
LLSFKKRHPKKFTCALWLVFFLAYTVLTVLIWLVQLIFRKGNVFQMLLQLIILDIAIA
LVNVAIAFTFEIYLSQKAAIEIRDEGLNNLDTA"
gene complement(<64977..>66614)
/gene="CLN2"
/locus_tag="YPL256C"
/db_xref="GeneID:855819"
mRNA complement(<64977..>66614)
/gene="CLN2"
/locus_tag="YPL256C"
/product="cyclin CLN2"
/transcript_id="NM_001184070.1"
/db_xref="GeneID:855819"
CDS complement(64977..66614)
/gene="CLN2"
/locus_tag="YPL256C"
/experiment="EXISTENCE:direct assay:GO:0000307
cyclin-dependent protein kinase holoenzyme complex
[PMID:2142620]"
/experiment="EXISTENCE:direct assay:GO:0001932 regulation
of protein phosphorylation
[PMID:10409718|PMID:7862657|PMID:8387915]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11080155|PMID:11792824|PMID:10611233]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11509671|PMID:11080155|PMID:10611233|PMID:11792824]"
/experiment="EXISTENCE:direct assay:GO:0016538
cyclin-dependent protein serine/threonine kinase regulator
activity [PMID:7862657|PMID:10409718|PMID:8387915]"
/experiment="EXISTENCE:genetic interaction:GO:0000321
re-entry into mitotic cell cycle after pheromone arrest
[PMID:9927449]"
/experiment="EXISTENCE:genetic interaction:GO:0007089
traversing start control point of mitotic cell cycle
[PMID:2574633|PMID:2147225]"
/experiment="EXISTENCE:mutant phenotype:GO:0005737
cytoplasm [PMID:11509671]"
/experiment="EXISTENCE:mutant phenotype:GO:0007089
traversing start control point of mitotic cell cycle
[PMID:2569741]"
/note="G1 cyclin involved in regulation of the cell cycle;
activates Cdc28p kinase to promote the G1 to S phase
transition; late G1 specific expression depends on
transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF
(Swi6p-Swi4p); CLN2 has a paralog, CLN1, that arose from
the whole genome duplication; cell cycle arrest phenotype
of the cln1 cln2 cln3 triple null mutant is complemented
by any of human cyclins CCNA2, CCNB1, CCNC, CCND1, or
CCNE1"
/codon_start=1
/product="cyclin CLN2"
/protein_id="NP_015067.1"
/db_xref="GeneID:855819"
/db_xref="SGD:S000006177"
/translation="MASAEPRPRMGLVINAKPDYYPIELSNAELLSHFEMLQEYHQEI
STNVIAQSCKFKPNPKLIDQQPEMNPVETRSNIITFLFELSVVTRVTNGIFFHSVRLY
DRYCSKRIVLRDQAKLVVATCLWLAAKTWGGCNHIINNVVIPTGGRFYGPNPRARIPR
LSELVHYCGDGQVFDESMFLQMERHILDTLNWNIYEPMINDYVLNVDENCLMQYELYE
NQVTYDKQCSEKRQSQLSQDSDATVDERPYQNEEEEEEDLKLKIKLINLKKFLIDVSA
WQYDLLRYELFEVSHGIFSIINQFTNQDHGPFLMTPMTSESKNGEILSTLMNGIVSIP
NSLMEVYKTVNGVLPFINQVKEYHLDLQRKLQIASNLNISRKLTISTPSCSFENSNST
SIPSPASSSQSHTPMRNMSSLSDNSVFSRNMEQSSPITPSMYQFGQQQSNSICGSTVS
VNSLVNTNNKQRIYEQITGPNSNNATNDYIDLLNLNESNKENQNPATAHYLNGGPPKT
SFINHGMFPSPTGTINSGKSSSASSLISFGMGNTQVI"
gene <67725..>68882
/gene="BBP1"
/locus_tag="YPL255W"
/db_xref="GeneID:855820"
mRNA <67725..>68882
/gene="BBP1"
/locus_tag="YPL255W"
/product="Bbp1p"
/transcript_id="NM_001184069.1"
/db_xref="GeneID:855820"
CDS 67725..68882
/gene="BBP1"
/locus_tag="YPL255W"
/experiment="EXISTENCE:direct assay:GO:0005198 structural
molecule activity [PMID:20139001]"
/experiment="EXISTENCE:direct assay:GO:0005823 central
plaque of spindle pole body [PMID:10654940]"
/experiment="EXISTENCE:genetic interaction:GO:0030474
spindle pole body duplication [PMID:10654940]"
/experiment="EXISTENCE:mutant phenotype:GO:0030474 spindle
pole body duplication [PMID:10654940]"
/experiment="EXISTENCE:mutant phenotype:GO:0071988 protein
localization to spindle pole body
[PMID:11952896|PMID:14767068]"
/note="Protein required for the spindle pole body (SPB)
duplication; localizes at the cytoplasmic side of the
central plaque periphery of the SPB; forms a complex with
a nuclear envelope protein Mps2p and SPB components Spc29p
and Kar1p; required for mitotic functions of Cdc5p"
/codon_start=1
/product="Bbp1p"
/protein_id="NP_015068.1"
/db_xref="GeneID:855820"
/db_xref="SGD:S000006176"
/translation="MNQEDNTGGGGIFGLFKWTKDALFGTDISPSMKYKDQEERRDRS
RYAQDDTNFSMKFGNDSNRRSTNLSRSNSWSGLDSTLHRKYELLPEYNENGFNSIVNG
DHHSKERIRSLRSPAPIVPREPLRNEPTDTFGHRLHTKRRTINELSNSQIPFIPPQED
DPLLSKLFNKDGVNEVRRSPYKLSVKDIPGKFPSPLTKRDEIDNYYVRDEDACHKNRE
YKKAYFDLFAQMDLNSRDLEDLCEDVREQREQFHRNEQTYKQAYEEMRAELVNELKKS
KTLFENYYSLGQKYKSLKKVLDQTISHEAELATSRERLYQEEDLKNFEIQTLKQRLSD
LELKYTNLQIEKDMQRDNYESEIHDLLLQLSLRNNERKDTSAGSNIFSTGQ"
gene <69485..>70951
/gene="HFI1"
/locus_tag="YPL254W"
/gene_synonym="ADA1; GAN1; SRM12; SUP110"
/db_xref="GeneID:855821"
mRNA <69485..>70951
/gene="HFI1"
/locus_tag="YPL254W"
/gene_synonym="ADA1; GAN1; SRM12; SUP110"
/product="Hfi1p"
/transcript_id="NM_001184068.1"
/db_xref="GeneID:855821"
CDS 69485..70951
/gene="HFI1"
/locus_tag="YPL254W"
/gene_synonym="ADA1; GAN1; SRM12; SUP110"
/experiment="EXISTENCE:direct assay:GO:0000124 SAGA
complex [PMID:9674426]"
/experiment="EXISTENCE:direct assay:GO:0006325 chromatin
organization [PMID:9674426]"
/experiment="EXISTENCE:direct assay:GO:0046695 SLIK
(SAGA-like) complex [PMID:12446794]"
/experiment="EXISTENCE:mutant phenotype:GO:0003712
transcription coregulator activity [PMID:9154821]"
/experiment="EXISTENCE:mutant phenotype:GO:0003713
transcription coactivator activity [PMID:10048020]"
/experiment="EXISTENCE:mutant phenotype:GO:0006366
transcription by RNA polymerase II [PMID:9154821]"
/note="Adaptor protein required for structural integrity
of the SAGA complex; a histone
acetyltransferase-coactivator complex that is involved in
global regulation of gene expression through acetylation
and transcription functions"
/codon_start=1
/product="Hfi1p"
/protein_id="NP_015069.1"
/db_xref="GeneID:855821"
/db_xref="SGD:S000006175"
/translation="MSAIQSPAPKPLQPTYPAASPASTNAYMKPGLIGSPAVSNHTEP
NNGNNETAEPQGPNQRIDLGAMIEELTSLLGKESWTKYAQIISLFILGKLSRKELSNE
LELVFSPSAASLEKSNTNHHHSLVRLHNQLLLGIFANSLRENPLGRNGNESSWGFGNG
SNNPNNKLKRINKHNSQIEVYKKIVMSLPLNDRNRLKMITKEAGKRGFIFCSVFQARL
NNIPKIPIVTNPESLKRVKSNNLKTPLEWSQDIMNGFNVPLASESHSLPDTDSFYLRM
VGIAREHGLVGTVDARCVELISLALDQYLKNIIEFTIDTVRYRRKKYSDYYDLNESGL
YKSVSEMAADKRDAKIKQLDDDKNEDECADEAKSINNGNNSSKDDIGDISMSSITKAG
EAVNEELHENRTISLTNEDIYDSLSIFPNLVEPSGSYYALTNLGLVNDDELVDMKSNI
DDLPDFLNEKPTFTPLDERNVGTRHELNWLIKGILTED"
gene complement(<71063..>73006)
/gene="VIK1"
/locus_tag="YPL253C"
/db_xref="GeneID:855822"
mRNA complement(<71063..>73006)
/gene="VIK1"
/locus_tag="YPL253C"
/product="Vik1p"
/transcript_id="NM_001184067.1"
/db_xref="GeneID:855822"
CDS complement(71063..73006)
/gene="VIK1"
/locus_tag="YPL253C"
/experiment="EXISTENCE:direct assay:GO:0005816 spindle
pole body [PMID:11729143|PMID:10087265]"
/experiment="EXISTENCE:direct assay:GO:0005871 kinesin
complex [PMID:10087265]"
/experiment="EXISTENCE:direct assay:GO:0008017 microtubule
binding [PMID:22734002]"
/experiment="EXISTENCE:genetic interaction:GO:0007064
mitotic sister chromatid cohesion [PMID:14742714]"
/experiment="EXISTENCE:mutant phenotype:GO:0007017
microtubule-based process [PMID:10087265]"
/experiment="EXISTENCE:mutant phenotype:GO:0007020
microtubule nucleation [PMID:33752231]"
/experiment="EXISTENCE:mutant phenotype:GO:0007064 mitotic
sister chromatid cohesion [PMID:14742714]"
/experiment="EXISTENCE:mutant phenotype:GO:0036503 ERAD
pathway [PMID:39841550]"
/experiment="EXISTENCE:physical interaction:GO:0007017
microtubule-based process [PMID:10087265]"
/note="Subunit of a kinesin-14 heterodimeric motor with
Kar3p; localizes Kar3p at mitotic spindle poles; has a
structure similar to a kinesin motor domain but lacks an
ATP-binding site and is catalytically inactive; binds
microtubules; required for sister chromatid cohesion and
for ER-associated degradation (ERAD); VIK1 has a paralog,
CIK1, that arose from the whole genome duplication"
/codon_start=1
/product="Vik1p"
/protein_id="NP_015070.1"
/db_xref="GeneID:855822"
/db_xref="SGD:S000006174"
/translation="MASQQNKHAFLSKNRIFHNPDNVSSSKSRNLMDITNTTNTMNGS
RPSSMKSSLALPPVKDSFPSVSRSASLNINMSKIKDLKDRQDKIRFQRHTLRTQLIEC
EREIKTIKFRDLNKSRFELYKKKSKQAKYLKQVRDLTQNLNSKDGERADLIKKNKSAL
ATLQAELDQNLILKRQESQELYNNKLIFWENELQIMENVEPDHEITEEISQLKKTLQE
LNINWANLQKQNLERQVNHESQLRKDFIAFKEAKLKSMENLTNKHRELLDQIATLQSE
SEKLHKEIMDIDRQAEYSEQNISEINENIKQLELANNPLISKSLQNSQDLEHLQNQME
NLKEMASKQEKFYNDTYNTVEKELLRSRRLENSIIEQKGTMRCYAYVMEQNLPENLLF
DYENGVITQGLSEHVYKFNRVIPHLKVSEDKFFTQEYSVYHDMCLNQKKNFNLISLST
TPHGSLRESLIKFLAEKDTIYQKQYVITLQFVFLSDDEFSQDMLLDYSHNDKDSIKLK
FEKHSISLDSKLVIIENGLEDLPLNFSCDEHPNLPHSGMGIIKVQFFPRDSKSDGNND
PVPVDFYFIELNNLKSIEQFDKSIFKKESCETPIALVLKKLISDTKSFFLLNLNDSKN
VNKLLTISEEVQTQLCKRKKKLT"
rep_origin 73032..73227
/note="ARS1605; Autonomously Replicating Sequence"
/db_xref="SGD:S000118412"
gene complement(<73363..>73881)
/gene="YAH1"
/locus_tag="YPL252C"
/db_xref="GeneID:855824"
mRNA complement(<73363..>73881)
/gene="YAH1"
/locus_tag="YPL252C"
/product="adrenodoxin"
/transcript_id="NM_001184066.1"
/db_xref="GeneID:855824"
CDS complement(73363..73881)
/gene="YAH1"
/locus_tag="YPL252C"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:10375636]"
/experiment="EXISTENCE:genetic interaction:GO:0006784 heme
A biosynthetic process [PMID:11788607]"
/experiment="EXISTENCE:genetic interaction:GO:0016653
oxidoreductase activity, acting on NAD(P)H, heme protein
as acceptor [PMID:11788607]"
/experiment="EXISTENCE:mutant phenotype:GO:0006744
ubiquinone biosynthetic process
[PMID:20534343|PMID:21944752]"
/experiment="EXISTENCE:mutant phenotype:GO:0006784 heme A
biosynthetic process [PMID:11788607]"
/experiment="EXISTENCE:mutant phenotype:GO:0016226
iron-sulfur cluster assembly [PMID:12970193]"
/experiment="EXISTENCE:mutant phenotype:GO:0016653
oxidoreductase activity, acting on NAD(P)H, heme protein
as acceptor [PMID:11788607]"
/note="Ferredoxin of the mitochondrial matrix; required
for formation of cellular iron-sulfur proteins; involved
in heme A biosynthesis; human homolog FDX1L can complement
yeast by allowing growth during down-regulation of yeast
YAH1"
/codon_start=1
/product="adrenodoxin"
/protein_id="NP_015071.1"
/db_xref="GeneID:855824"
/db_xref="SGD:S000006173"
/translation="MLKIVTRAGHTARISNIAAHLLRTSPSLLTRTTTTTRFLPFSTS
SFLNHGHLKKPKPGEELKITFILKDGSQKTYEVCEGETILDIAQGHNLDMEGACGGSC
ACSTCHVIVDPDYYDALPEPEDDENDMLDLAYGLTETSRLGCQIKMSKDIDGIRVALP
QMTRNVNNNDFS"
gene complement(<74309..>74719)
/gene="ATG41"
/locus_tag="YPL250C"
/gene_synonym="ICY2"
/db_xref="GeneID:855825"
mRNA complement(<74309..>74719)
/gene="ATG41"
/locus_tag="YPL250C"
/gene_synonym="ICY2"
/product="Atg41p"
/transcript_id="NM_001184064.1"
/db_xref="GeneID:855825"
CDS complement(74309..74719)
/gene="ATG41"
/locus_tag="YPL250C"
/gene_synonym="ICY2"
/experiment="EXISTENCE:mutant phenotype:GO:0000045
autophagosome assembly [PMID:26565778]"
/experiment="EXISTENCE:mutant phenotype:GO:0000422
autophagy of mitochondrion [PMID:19793921]"
/experiment="EXISTENCE:mutant phenotype:GO:0006995
cellular response to nitrogen starvation [PMID:26565778]"
/experiment="EXISTENCE:mutant phenotype:GO:0016236
macroautophagy [PMID:26565778]"
/experiment="EXISTENCE:mutant phenotype:GO:0032258
cytoplasm to vacuole targeting by the Cvt pathway
[PMID:26565778]"
/note="hypothetical protein; required for selective and
nonselective autophagy, and mitophagy; regulates the rate
of autophagosome formation; interacts with Atg9p, and has
a similar peri-mitochondrial localization; elevated
Gcn4p-dependent expression under autophagy-inducing
conditions; mobilized into polysomes upon a shift from a
fermentable to nonfermentable carbon source; potential
Cdc28p substrate; ATG41 has a paralog, ICY1, that arose
from the whole genome duplication"
/codon_start=1
/product="Atg41p"
/protein_id="NP_015073.1"
/db_xref="GeneID:855825"
/db_xref="SGD:S000006171"
/translation="MSSVESSPISRYEDEVFPLSFSNVAFEPPMLSHSPDRSTYADDF
SQSYQQELLTFPLSYPIVDESECTHTKDKTDSNIITSTEDDCMFDMEFNGNAASAVAA
ASKESNSASGFAFASNDAFANVAQQNYRLWLSSV"
gene complement(<75699..>76239)
/gene="RPL36B"
/locus_tag="YPL249C-A"
/db_xref="GeneID:855826"
mRNA complement(join(<75699..75985,76224..>76239))
/gene="RPL36B"
/locus_tag="YPL249C-A"
/product="60S ribosomal protein eL36 RPL36B"
/transcript_id="NM_001184312.1"
/db_xref="GeneID:855826"
CDS complement(join(75699..75985,76224..76239))
/gene="RPL36B"
/locus_tag="YPL249C-A"
/experiment="EXISTENCE:direct assay:GO:0002181 cytoplasmic
translation [PMID:18782943]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:6337137]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:18782943]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:18782943]"
/experiment="EXISTENCE:mutant phenotype:GO:0000470
maturation of LSU-rRNA [PMID:37865285]"
/experiment="EXISTENCE:mutant phenotype:GO:0180023
cytosolic large ribosomal subunit assembly
[PMID:37865285]"
/note="Ribosomal 60S subunit protein L36B; binds to 5.8 S
rRNA; homologous to mammalian ribosomal protein L36, no
bacterial homolog; RPL36B has a paralog, RPL36A, that
arose from the whole genome duplication"
/codon_start=1
/product="60S ribosomal protein eL36 RPL36B"
/protein_id="NP_015074.1"
/db_xref="GeneID:855826"
/db_xref="SGD:S000006438"
/translation="MAVKTGIAIGLNKGKKVTQMTPAPKISYKKGAASNRTKFVRSLV
REIAGLSPYERRLIDLIRNSGEKRARKVAKKRLGSFTRAKAKVEEMNNIIAASRRH"
gene complement(<76669..>79353)
/gene="GYP5"
/locus_tag="YPL249C"
/db_xref="GeneID:855827"
mRNA complement(<76669..>79353)
/gene="GYP5"
/locus_tag="YPL249C"
/product="GTPase-activating protein GYP5"
/transcript_id="NM_001184063.1"
/db_xref="GeneID:855827"
CDS complement(76669..79353)
/gene="GYP5"
/locus_tag="YPL249C"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:15331637]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005798
Golgi-associated vesicle [PMID:15331637]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:12189143|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:15331637]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:15331637|PMID:22842922|PMID:32015451]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:22842922|PMID:15331637|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:35134079]"
/experiment="EXISTENCE:mutant phenotype:GO:0005096 GTPase
activator activity [PMID:15802519|PMID:12189143]"
/experiment="EXISTENCE:mutant phenotype:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:15802519]"
/experiment="EXISTENCE:mutant phenotype:GO:0016192
vesicle-mediated transport [PMID:12189143]"
/note="GTPase-activating protein (GAP) for yeast Rab
family members; involved in ER to Golgi trafficking;
exhibits GAP activity toward Ypt1p that is stimulated by
Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p
and Rvs167p; involved in recruiting Rvs167p to the bud tip
during polarized growth; relocalizes from bud neck to
cytoplasm upon DNA replication stress; GYP5 has a paralog,
GYL1, that arose from the whole genome duplication"
/codon_start=1
/product="GTPase-activating protein GYP5"
/protein_id="NP_015075.1"
/db_xref="GeneID:855827"
/db_xref="SGD:S000006170"
/translation="MSSDKSIEKNTDTIASEVHEGDNHSNNLGSMEEEIKSTPSDQYE
EIAIIPTEPLHSDKELNDKQQSLGHEAPTNVSREEPIGISGDEDTQITEQNVNEQRQE
TREPSSEIDLNEPLDVEKDVTTDVQAPNGLNIEKEYDAVKENEKVYADTKEVVSSPEN
REVTGKNSGGEKSSSSKFLDDESGTTTAANANDISISSEVTPERSSENDNNQIHITNE
VAAGINLNENKEQKAAIEDGPVTAENLSSETARKVPPIPTQIINEKGDNSSENEVSAI
PTTSSPPLPPRQNVATSTSPKLPPRGKQREQPPKTKNAVPPPLEEEMKSEKFRKNFEE
TKRNSYHHVPLTGSKTAQLESTAEINLIASRYRKTSHHLNKEGEETRESLQEGQSFLK
STFTSFLENLSEYNEVENVNEEDREMFKIDWSFWTQVVNDYATVASNEPENLEAHVTN
GIPPQIRGIIWQLMANSKSREMEDIYETLLDTECLHEATIRRDLRRTKFVAEDKMESL
YKVIKVYSVYDPDVGYTQGMGFIAAPLLINCENEAESFGLLVGLMKNYGLRELFLPGM
PGLMLMLYQFDRLLEEHSPSLYNRLIREGISSTMYATQWFLTFFAYKFPLEFVLRIFD
IVFVEGIEVLLKFAVNLMLKNEETLVKLRFDELLDFLKDELFNYYLMGNQDDASVVQM
GLSNGNSFKGNDDGTFSYNVDLFVHDAMTGVYITPLTLRRYRGEYVEIHEKEQKKEDH
YESLRIQNHQLQREAQKLEHDYSILNKENISAANELIQNRLNMEMLLDEKNDLINTIT
DIKSQIEEEIRKQNLPNPDASLPKADLREDLERTISRNNEVMRENGQLEERITELQAE
IDELININKEQVSTASLLERDSKAKGRKGWTGFKKVFK"
gene 79562..82648
/locus_tag="YNCP0002W"
/db_xref="GeneID:65052918"
ncRNA 79562..82648
/ncRNA_class="antisense_RNA"
/locus_tag="YNCP0002W"
/product="GAL4 lncRNA"
/note="Antisense to GAL4/YPL248C; influences expression of
GAL4 mRNA"
/transcript_id="NR_172478.1"
/db_xref="GeneID:65052918"
/db_xref="SGD:S000303815"
gene complement(<79711..>82356)
/gene="GAL4"
/locus_tag="YPL248C"
/gene_synonym="GAL81"
/db_xref="GeneID:855828"
mRNA complement(<79711..>82356)
/gene="GAL4"
/locus_tag="YPL248C"
/gene_synonym="GAL81"
/product="galactose-responsive transcription factor GAL4"
/transcript_id="NM_001184062.1"
/db_xref="GeneID:855828"
CDS complement(79711..82356)
/gene="GAL4"
/locus_tag="YPL248C"
/gene_synonym="GAL81"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:3886158]"
/experiment="EXISTENCE:direct assay:GO:0001228 DNA-binding
transcription activator activity, RNA polymerase
II-specific [PMID:3080805]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:6091123]"
/experiment="EXISTENCE:direct assay:GO:0045944 positive
regulation of transcription by RNA polymerase II
[PMID:3886158]"
/experiment="EXISTENCE:direct assay:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:14871930]"
/experiment="EXISTENCE:genetic interaction:GO:0000981
DNA-binding transcription factor activity, RNA polymerase
II-specific [PMID:14871930]"
/experiment="EXISTENCE:mutant phenotype:GO:0000981
DNA-binding transcription factor activity, RNA polymerase
II-specific [PMID:14871930]"
/experiment="EXISTENCE:mutant phenotype:GO:0001228
DNA-binding transcription activator activity, RNA
polymerase II-specific [PMID:3080805]"
/experiment="EXISTENCE:mutant phenotype:GO:0006012
galactose metabolic process [PMID:6350827]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:6350827]"
/experiment="EXISTENCE:physical interaction:GO:0000981
DNA-binding transcription factor activity, RNA polymerase
II-specific [PMID:14871930]"
/experiment="EXISTENCE:physical interaction:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:14871930]"
/note="DNA-binding transcription factor required for
activating GAL genes; responds to galactose; repressed by
Gal80p and activated by Gal3p"
/codon_start=1
/product="galactose-responsive transcription factor GAL4"
/protein_id="NP_015076.1"
/db_xref="GeneID:855828"
/db_xref="SGD:S000006169"
/translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIP
LDFMPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTN
SNVNRLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASK
DQWQILFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSR
YTQWRQKTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQL
SLLYGRSIQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVT
AEKSPICAKKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWK
QLSLIIYVLRDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMD
NHNVTPYFAWNCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLA
TFKIQTCEKYIQVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTL
PEENVNNISVKYVSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSH
RSVTPFLGQQQQLQSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQ
TNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMF
NTTTMDDVYNYLFDDEDTPPNPKKE"
gene complement(<82625..>84196)
/locus_tag="YPL247C"
/db_xref="GeneID:855829"
mRNA complement(<82625..>84196)
/locus_tag="YPL247C"
/product="uncharacterized protein"
/transcript_id="NM_001184061.1"
/db_xref="GeneID:855829"
CDS complement(82625..84196)
/locus_tag="YPL247C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to the cytoplasm and
nucleus; similar to the petunia WD repeat protein an11;
overexpression causes a cell cycle delay or arrest"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015077.1"
/db_xref="GeneID:855829"
/db_xref="SGD:S000006168"
/translation="MDPFHNGNKRSSISFGSSQRQPYNKNNYLSGTNGPSSAAQDQGR
GPSPFGMSGNTTNGGNSKRNSGCDLSATYYASRSPMYSPLDFSPPVFSPNHSQLQQAR
GYAANIPVVSNLMNPSMASVCEYQSHYPLFGLDWSADDYVCLGSYKEDSRNKLQVLHS
NDLLSWESVVDADVVYPVSKIQWVPSQLYPRKLATCSDSLRIWSVSPEERQFQEQINL
SLCKYNRQHPANPAAADDMKVIGTFPPITSFDWNTVDTNLIISSSIDTTCIVWDLQSS
HYVKTQLIAHDSEVFDVRFLTKSTQLFASCGGDGSVRVFDLRSLAHSTIIYEPPSSSV
SGATAGTITPSSKGSDALLRLEPSPYDPNVLATFAADSNKIIILDMRNPESPILNLQG
HGSSVNGIKWHPTKRNVLLSCGDDCQVLYWDLNSSFMEINAAGSKSPSIHGTSLEDPD
GDTEMTDGGAGSGLNEDPLSLNNNSKQVCKTLETPNMMYANKTQEINNIAWRPQRGDW
FGCVSGKKFQNVRVL"
gene complement(<84509..>85297)
/gene="RBD2"
/locus_tag="YPL246C"
/db_xref="GeneID:855830"
mRNA complement(<84509..>85297)
/gene="RBD2"
/locus_tag="YPL246C"
/product="putative rhomboid protease RBD2"
/transcript_id="NM_001184060.1"
/db_xref="GeneID:855830"
CDS complement(84509..85297)
/gene="RBD2"
/locus_tag="YPL246C"
/EC_number="3.4.21.105"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0034399 nuclear
periphery [PMID:25817432]"
/note="Possible rhomboid protease; has similarity to
eukaryotic rhomboid proteases including Pcp1p"
/codon_start=1
/product="putative rhomboid protease RBD2"
/protein_id="NP_015078.1"
/db_xref="GeneID:855830"
/db_xref="SGD:S000006167"
/translation="MNWKSYVFPGGHPPAALTTGLVVFLTAIYLLSFIFALREDLSLA
PESLFKLQMSRLSLYPLIHLSLPHLLFNVLAIWAPLNLFEETHGTVYTGVFLNLSALF
AGILYCLLGKLLYPEALVAGASGWCFTLFAYYSFKESQIRPRTRIFRTDYSIPTLYTP
LVLLVAIAVVIPGSSFWGHFFGLCVGYAIGYKESWFNKITPPGWIITKIEKSLDGLIR
LIPWGIKYYRDEDIDRTKDYEPLMSTETPLPLHNDNSGTVLGTA"
gene <85586..>86950
/locus_tag="YPL245W"
/db_xref="GeneID:855831"
mRNA <85586..>86950
/locus_tag="YPL245W"
/product="uncharacterized protein"
/transcript_id="NM_001184059.1"
/db_xref="GeneID:855831"
CDS 85586..86950
/locus_tag="YPL245W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to both the nucleus and the
cytoplasm"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015079.1"
/db_xref="GeneID:855831"
/db_xref="SGD:S000006166"
/translation="MHGPTSKAISRNVRSVKRPRRAPRPVVSTQAMNKLSNVTLSAEQ
EKLRERVLSFMRSNLSQYKSDWKHPAMFVIQGDAGTGKSVILNSLFNEIQKLSQFSPS
SEDILHGTHNYLVVNHPEMLKLYIRISDSFKYISKSSLERPTSLINNLQKRKVMADVV
IVDEAHLLATSKDAFKRFYGENHLKDLMSLCKVLVLVYDDKQALRMGSYWDEGSNNGA
TLKDFYNEIPPKSRDWYTLKQQFRVAAPQNVLNWIDQISVAGKIPPIESVLSKGNADC
ADDKIKNFDFKIWDDCGAMYEAIKEKDRQYGQCRMLSTYDFPYRLDGKDYYVECGDNF
KVRWDRYTPREVTPWSERCDTIDEVGSVYTIQGFDLNYAGVILGRSIGYDAANDCIKL
RPELYDDRAGFTKKKNIHNAEDVKQKIIMNSINVLLTRGVRGLYVYAYDPELRERLLR
PSKK"
gene complement(<87014..>88033)
/gene="HUT1"
/locus_tag="YPL244C"
/db_xref="GeneID:855832"
mRNA complement(<87014..>88033)
/gene="HUT1"
/locus_tag="YPL244C"
/product="UDP-galactose transporter HUT1"
/transcript_id="NM_001184058.1"
/db_xref="GeneID:855832"
CDS complement(87014..88033)
/gene="HUT1"
/locus_tag="YPL244C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0015786
UDP-glucose transmembrane transport [PMID:18693752]"
/experiment="EXISTENCE:mutant phenotype:GO:0015786
UDP-glucose transmembrane transport [PMID:18693752]"
/experiment="EXISTENCE:mutant phenotype:GO:0072334
UDP-galactose transmembrane transport [PMID:11284009]"
/note="Protein with a role in UDP-galactose transport to
the Golgi lumen; has similarity to human UDP-galactose
transporter UGTrel1, exhibits a genetic interaction with
S. cerevisiae ERO1"
/codon_start=1
/product="UDP-galactose transporter HUT1"
/protein_id="NP_015080.1"
/db_xref="GeneID:855832"
/db_xref="SGD:S000006165"
/translation="MAGSTSSLVICAIGIYATFLTWALVQEPLATRTWPNSMGKFQFP
NVISLIQASVAMMMGYLYLNWKKVEYPPRKMIKDHWKQLMLISFTQSSSGPLATTSLK
HVDYLTYMLAKSCKMIPVLLVHLLLYRTPIASQKKVVALLVSLGVTIFTIGGNDGKKL
KRSFNESGNDNKLQGFGLLFSSLFLDGLTNATQDKLLKANKAKEKGKQTLITGAHLMF
TLNLFVILWNILYFIVIDCKQWDNAVSVLTMDPQVWGYLMLYSFCGAMGQCFIFYTLE
QFGSLVLIMITVTRKMVSMILSIIVFGKSVRFQQWVGMFIVFGGITWEALNKKKANIP
KAKSA"
gene <88517..>90316
/gene="SRP68"
/locus_tag="YPL243W"
/db_xref="GeneID:855833"
mRNA <88517..>90316
/gene="SRP68"
/locus_tag="YPL243W"
/product="signal recognition particle subunit SRP68"
/transcript_id="NM_001184057.1"
/db_xref="GeneID:855833"
CDS 88517..90316
/gene="SRP68"
/locus_tag="YPL243W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005786 signal
recognition particle, endoplasmic reticulum targeting
[PMID:7925282]"
/experiment="EXISTENCE:direct assay:GO:0006614
SRP-dependent cotranslational protein targeting to
membrane [PMID:10921896]"
/experiment="EXISTENCE:direct assay:GO:0034399 nuclear
periphery [PMID:22842922]"
/note="Core component of the signal recognition particle
(SRP) complex; SRP complex functions in targeting nascent
secretory proteins to the endoplasmic reticulum (ER)
membrane; relocalizes from cytoplasm to the nuclear
periphery upon DNA replication stress"
/codon_start=1
/product="signal recognition particle subunit SRP68"
/protein_id="NP_015081.1"
/db_xref="GeneID:855833"
/db_xref="SGD:S000006164"
/translation="MVAYSPIIATYGNRAEQFLETDSDFAKYHAKLNKKLQHLRSRCH
LVTKDTKKYSSKNKYGEINSEDYDNKTKLIGVLILLHAERDLALAETLKLRARQRGKL
KKSEEKVLSTRLKKACKTADKLVNVTQNEQQWITRAQYLAFAKLVHSEYLINGKRFKR
KDNAKISNNLALVFAALEHLKNLSLLAEEVVDNIVNKYQYSLKQYAGNLITTPEINNF
IVERVQSDENKDDELVKLLLDNGFNMKKITTSTEDQKVTTNINWRSFNAKIIDAEVAQ
FLEQGLSIHPTQITQYTQRLSKLEKALDRHEFFIANHDDQDDIDEMVENSSENNQIIL
AYIKYNILLTSISRERDLFTHLWNQWLKLNTSLPSKLTKYKEMERIVKNLTKYLSDIM
ELPGVYSDDELLSQLDLCKLYFQLFLNTGCLSVLYQSKGRYMEALALYVDAYRRLENK
LSEIESLDEILLPANLLSLNSVRSLQKRIENGGNSVITLAEYEKRNHGGSLGKYDLTV
IEKLDSKKILPTDIQLKNLFPLKPKMLPIPSKPTLFDLAFNYITYDKQEPSASQVKDS
VTETESISQTPISNEQTEGEPKKKRGFLGLFGR"
gene complement(<90622..>95109)
/gene="IQG1"
/locus_tag="YPL242C"
/gene_synonym="CYK1"
/db_xref="GeneID:855834"
mRNA complement(<90622..>95109)
/gene="IQG1"
/locus_tag="YPL242C"
/gene_synonym="CYK1"
/product="Iqg1p"
/transcript_id="NM_001184056.1"
/db_xref="GeneID:855834"
CDS complement(90622..95109)
/gene="IQG1"
/locus_tag="YPL242C"
/gene_synonym="CYK1"
/experiment="EXISTENCE:direct assay:GO:0000142 cellular
bud neck contractile ring
[PMID:10873803|PMID:9442111|PMID:9950677|PMID:9382845]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:11082046|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0051015 actin
filament binding [PMID:9382845|PMID:9950677]"
/experiment="EXISTENCE:direct assay:GO:0120155 MIH complex
[PMID:24895401]"
/experiment="EXISTENCE:mutant phenotype:GO:0000281 mitotic
cytokinesis [PMID:21173112]"
/experiment="EXISTENCE:mutant phenotype:GO:0051015 actin
filament binding [PMID:9382845|PMID:9950677]"
/experiment="EXISTENCE:mutant phenotype:GO:0072741 protein
localization to cell division site [PMID:21173112]"
/experiment="EXISTENCE:mutant phenotype:GO:1903471
regulation of mitotic actomyosin contractile ring
contraction
[PMID:24895401|PMID:19109423|PMID:26085509|PMID:9950677]"
/experiment="EXISTENCE:mutant phenotype:GO:1903475 mitotic
actomyosin contractile ring assembly
[PMID:11082046|PMID:21173112]"
/experiment="EXISTENCE:mutant phenotype:GO:1903479 mitotic
actomyosin contractile ring assembly actin filament
organization [PMID:9382845|PMID:9950677|PMID:9442111]"
/experiment="EXISTENCE:physical interaction:GO:0005516
calmodulin binding [PMID:9950677]"
/experiment="EXISTENCE:physical interaction:GO:0032027
myosin light chain binding [PMID:10873803]"
/experiment="EXISTENCE:physical interaction:GO:0032033
myosin II light chain binding [PMID:11082046]"
/experiment="EXISTENCE:physical interaction:GO:0032038
myosin II heavy chain binding [PMID:11082046]"
/experiment="EXISTENCE:physical interaction:GO:0120155 MIH
complex [PMID:24895401]"
/note="Actin filament binding protein that enhances actin
ring formation; IQGAP family member required for assembly
and contraction of the AMR, prior to cytokinesis;
localizes to the contractile ring during anaphase in an
Mlc1p-dependent manner, recruiting Myo1p and Hof1p to the
site, and promoting cytokinetic core complex assembly;
specifies bud-site selection, localizing axial markers
Bud4p and Cdc12p; regulated by Cdc28p; relocalizes from
the bud neck to the cytoplasm upon replication stress"
/codon_start=1
/product="Iqg1p"
/protein_id="NP_015082.1"
/db_xref="GeneID:855834"
/db_xref="SGD:S000006163"
/translation="MTAYSGSPSKPGNNNSYLNRYVENLGTNVTPPLRPQSSSKINSS
LNIASPSHLKTKTSASNSSATILSKKVESSVSKLKPSLPNKLVGKYTVDLSNYSKIEL
RYYEFLCRVSEVKIWIEAVIEEALPSEIELCVGDSLRNGVFLAKLTQRINPDLTTVIF
PAGDKLQFKHTQNINAFFGLVEHVGVPDSFRFELQDLYNKKNIPQVFETLHILISMIN
KKWPGKTPALTNVSGQISFTKEEIAACKKAWPRIRDFKSLGTNINTAPASPEEPKEKR
SGLIKDFNKFERPNIPVEEILITPRKNITDANCSDFSNTPSPYNEAPKMSNLDVVVEK
RKFTPIEPSLLGPTPSLEYSPIKNKSLSYYSPTISKYLTYDTEFYTRRSRAREEDLNY
YQTFKYSPSHYSPMRRERMTEEQFLEKVVQLQNICRGVNTRFNLYIQKRLLNLFEQDI
LRFQACLRGNKFRVLSSMYLPIRRAKIDVPHVEAIQSRIKGSRIRYKYDKLKFTLSRF
SCTVELLQAYCRSKLLKTTVNTKLNDIEISHYPLTKLQSYMRASYVRKKVMSLNTKLN
DERESIMKFSAIIRGNVVRCSEDAILSAVHDVHKENISKLQSLIRGIFTRSCLASIIY
SLGKENCNIIQLSACIRGNAVRHKVQSLFAPENNLSETVHDLQGLVRGILVRYTLDLV
DDIVEYNNLALFQAFSRGALVRESLDQKSSFYKRNVRSVIMIQSWIRKSLQRSAYLEL
LDCPNPSLWAVKKFVHLLNGTATIEEVQNQLESCQASLDSENMKKERLLKSIRQQLNI
NGVLDKFGLLKDKDHELGISDSTIPKSKYQKYEKLFYMLQVDPSYWKLLYLKEPEFVA
KNVYMTFGTVNQRMNDRERSYFTRFVCEMLQNAINEAPSIESFLDNRSQFWQTILQDF
LRRESPEFFSIIVPVLDYLSDPVVDFESDPYKIYQEIHGFSSPQHCSPVDDASTKNKF
IDNLRCLWHAIEMVAEIYTRKVHTIPVEIRYLCTKIFCYAADKNIEEIDSLRAISSIL
VNVFVSEYLVNREYYGYKDSNVQKNNQKIDILMKSLATVFEIKNFDGFLDPLNQYANE
IKPHIKDVLYNVLVDPEYEQEGDRLIYLDMVSPSPKLELLTEKVLEISGKFEEYLNEF
PEADILHDILEKNLDNSSFPRSGRVTLELDASAYRFLVSDDKMRKIYDQVKRAFVYMM
QIEDVDTNLYDLSISTILPQDEPNFANFLEQNPKIRDDPMIQKLKPLKYFTLKNVTLK
KIHELESTGTFCSSDNKLQNFLNDIANTIKNPNYAIDYVTQEIYITKETLTKISEMNH
SLDIELSRLKKHVDHTIKDFQKAKDFSPVHKSKFGNFKNAVKKVQGRERSELQGMKFK
WNTKQLYERGVLKTIRGEKLAELTVKVFGSSGPKFPDIIFKISTSDGSRFGIQMIDKR
KGPDKRYSDDVDSFSFKDLIKTQVEPKIETWKLFHSNVVVNNSQLLHLIVSFFYKRNA
L"
gene complement(<95372..>96258)
/gene="CIN2"
/locus_tag="YPL241C"
/db_xref="GeneID:855835"
mRNA complement(join(<95372..96153,96234..>96258))
/gene="CIN2"
/locus_tag="YPL241C"
/product="GTPase-activating protein CIN2"
/transcript_id="NM_001184055.1"
/db_xref="GeneID:855835"
CDS complement(join(95372..96153,96234..96258))
/gene="CIN2"
/locus_tag="YPL241C"
/experiment="EXISTENCE:direct assay:GO:0005096 GTPase
activator activity [PMID:18376416]"
/experiment="EXISTENCE:genetic interaction:GO:0006457
protein folding [PMID:2407611]"
/experiment="EXISTENCE:genetic interaction:GO:0007021
tubulin complex assembly [PMID:9215891]"
/experiment="EXISTENCE:mutant phenotype:GO:0007021 tubulin
complex assembly [PMID:9215891]"
/note="GTPase-activating protein (GAP) for Cin4p; tubulin
folding factor C involved in beta-tubulin (Tub2p) folding;
mutants display increased chromosome loss and benomyl
sensitivity; human homolog RP2 complements yeast null
mutant"
/codon_start=1
/product="GTPase-activating protein CIN2"
/protein_id="NP_015083.1"
/db_xref="GeneID:855835"
/db_xref="SGD:S000006162"
/translation="MDFTAKIKELERELSETSDYKTLQKKTISLRSELNTLSHSLTSY
EKEHFSNDIENVLKSINAKLSESKGKKRLFSFKQKNSSSAVHKNVERTELANAPAYTT
TLKKHYVLEKGDSAFENLEFCTVTSTTDYSGNSALSGSLCFRNITKCVINLQRIFFQT
GSIFITDCTDSIIFLRSPSDKDFQIRLRDLKNCKILIEKLSPSIDCKQVVIIENCHKC
IFNASTRDHLIIQDFSNPFQSEETEDNSAFAFEDFDICNKDTMQLFRAYL"
gene complement(<96496..>98625)
/gene="HSP82"
/locus_tag="YPL240C"
/gene_synonym="HSP90"
/db_xref="GeneID:855836"
mRNA complement(<96496..>98625)
/gene="HSP82"
/locus_tag="YPL240C"
/gene_synonym="HSP90"
/product="Hsp90 family chaperone HSP82"
/transcript_id="NM_001184054.1"
/db_xref="GeneID:855836"
CDS complement(96496..98625)
/gene="HSP82"
/locus_tag="YPL240C"
/gene_synonym="HSP90"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:32920053]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:27385335|PMID:32920053|PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0006458 'de novo'
protein folding [PMID:10564510]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:12235160]"
/experiment="EXISTENCE:direct assay:GO:0032212 positive
regulation of telomere maintenance via telomerase
[PMID:17954556]"
/experiment="EXISTENCE:direct assay:GO:0043248 proteasome
assembly [PMID:12853471]"
/experiment="EXISTENCE:direct assay:GO:0051082 unfolded
protein binding [PMID:10564510]"
/experiment="EXISTENCE:mutant phenotype:GO:0000492 box C/D
snoRNP assembly [PMID:18268103]"
/experiment="EXISTENCE:mutant phenotype:GO:0006458 'de
novo' protein folding [PMID:9371781]"
/experiment="EXISTENCE:mutant phenotype:GO:0006970
response to osmotic stress [PMID:16487343]"
/experiment="EXISTENCE:mutant phenotype:GO:0016887 ATP
hydrolysis activity [PMID:27068472]"
/experiment="EXISTENCE:mutant phenotype:GO:0032212
positive regulation of telomere maintenance via telomerase
[PMID:17954556]"
/experiment="EXISTENCE:mutant phenotype:GO:0042026 protein
refolding [PMID:9371781]"
/experiment="EXISTENCE:mutant phenotype:GO:0043248
proteasome assembly [PMID:12853471]"
/experiment="EXISTENCE:mutant phenotype:GO:0051604 protein
maturation [PMID:27068472]"
/experiment="EXISTENCE:physical interaction:GO:0006626
protein targeting to mitochondrion [PMID:12526792]"
/note="Hsp90 chaperone; functionally redundant with
Hsc82p; required for pheromone signaling; negative
regulator of the Hsf1p-dependent heat shock response with
Cpr7p; docks with Tom70p for mitochondrial preprotein
delivery; promotes telomerase DNA binding, nucleotide
addition; promotes solubility of chaperone-substrate
complexes; DNA damage induced nuclear import is Aha1p
dependent; protein abundance increases in response to DNA
replication stress; human homolog, HSP90AB1, complements
null mutant"
/codon_start=1
/product="Hsp90 family chaperone HSP82"
/protein_id="NP_015084.1"
/db_xref="GeneID:855836"
/db_xref="SGD:S000006161"
/translation="MASETFEFQAEITQLMSLIINTVYSNKEIFLRELISNASDALDK
IRYKSLSDPKQLETEPDLFIRITPKPEQKVLEIRDSGIGMTKAELINNLGTIAKSGTK
AFMEALSAGADVSMIGQFGVGFYSLFLVADRVQVISKSNDDEQYIWESNAGGSFTVTL
DEVNERIGRGTILRLFLKDDQLEYLEEKRIKEVIKRHSEFVAYPIQLVVTKEVEKEVP
IPEEEKKDEEKKDEEKKDEDDKKPKLEEVDEEEEKKPKTKKVKEEVQEIEELNKTKPL
WTRNPSDITQEEYNAFYKSISNDWEDPLYVKHFSVEGQLEFRAILFIPKRAPFDLFES
KKKKNNIKLYVRRVFITDEAEDLIPEWLSFVKGVVDSEDLPLNLSREMLQQNKIMKVI
RKNIVKKLIEAFNEIAEDSEQFEKFYSAFSKNIKLGVHEDTQNRAALAKLLRYNSTKS
VDELTSLTDYVTRMPEHQKNIYYITGESLKAVEKSPFLDALKAKNFEVLFLTDPIDEY
AFTQLKEFEGKTLVDITKDFELEETDEEKAEREKEIKEYEPLTKALKEILGDQVEKVV
VSYKLLDAPAAIRTGQFGWSANMERIMKAQALRDSSMSSYMSSKKTFEISPKSPIIKE
LKKRVDEGGAQDKTVKDLTKLLYETALLTSGFSLDEPTSFASRINRLISLGLNIDEDE
ETETAPEASTAAPVEEVPADTEMEEVD"
gene <99484..>100086
/gene="YAR1"
/locus_tag="YPL239W"
/db_xref="GeneID:855837"
mRNA <99484..>100086
/gene="YAR1"
/locus_tag="YPL239W"
/product="Yar1p"
/transcript_id="NM_001184053.1"
/db_xref="GeneID:855837"
CDS 99484..100086
/gene="YAR1"
/locus_tag="YPL239W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22570489]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0051082 unfolded
protein binding [PMID:26112308]"
/experiment="EXISTENCE:genetic interaction:GO:0000056
ribosomal small subunit export from nucleus
[PMID:22570489]"
/experiment="EXISTENCE:genetic interaction:GO:0005634
nucleus [PMID:22570489]"
/experiment="EXISTENCE:genetic interaction:GO:0042274
ribosomal small subunit biogenesis [PMID:15611164]"
/experiment="EXISTENCE:mutant phenotype:GO:0000056
ribosomal small subunit export from nucleus
[PMID:22570489]"
/experiment="EXISTENCE:mutant phenotype:GO:0006970
response to osmotic stress [PMID:15611164]"
/experiment="EXISTENCE:mutant phenotype:GO:0032880
regulation of protein localization [PMID:22570489]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress [PMID:15611164]"
/experiment="EXISTENCE:mutant phenotype:GO:0042274
ribosomal small subunit biogenesis [PMID:15611164]"
/experiment="EXISTENCE:mutant phenotype:GO:0051082
unfolded protein binding [PMID:22570489]"
/experiment="EXISTENCE:physical interaction:GO:0032880
regulation of protein localization [PMID:22570489]"
/note="Nucleocytoplasmic shuttling assembly chaperone;
co-translationally associates with nascent Rps3p in the
cytoplasm and delivers it to pre-ribosomal subunits in the
nucleus; required for 40S ribosomal subunit nuclear
export, biogenesis and adaptation to osmotic and oxidative
stress; contains ankyrin-repeats; expression repressed by
heat shock"
/codon_start=1
/product="Yar1p"
/protein_id="NP_015085.1"
/db_xref="GeneID:855837"
/db_xref="SGD:S000006160"
/translation="MGLHSEPLDQEDQDTIILDARAGDLDSLKDIFTTLVSPELLSTC
KESESDSTALHMAAANGHIETVRYILETVSRANSAEDLKAFVNEVNKTGNTALHWASL
NGKLDVVKLLCDEYEADPFIRNKFGHDAIFEAENSGKEEVETYFLKKYDVEPEDDEED
TQTEGKNSVQITKGTEIEQVTKEATEALREETEKLNINKD"
gene <100496..>101353
/gene="SUI3"
/locus_tag="YPL237W"
/db_xref="GeneID:855838"
mRNA <100496..>101353
/gene="SUI3"
/locus_tag="YPL237W"
/product="translation initiation factor eIF2 subunit beta"
/transcript_id="NM_001184051.1"
/db_xref="GeneID:855838"
CDS 100496..101353
/gene="SUI3"
/locus_tag="YPL237W"
/experiment="EXISTENCE:direct assay:GO:0001731 formation
of translation preinitiation complex [PMID:12008673]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:9858542]"
/experiment="EXISTENCE:direct assay:GO:0005525 GTP binding
[PMID:14698289|PMID:16246727]"
/experiment="EXISTENCE:direct assay:GO:0005850 eukaryotic
translation initiation factor 2 complex [PMID:12137565]"
/experiment="EXISTENCE:direct assay:GO:0016282 eukaryotic
43S preinitiation complex [PMID:12008673]"
/experiment="EXISTENCE:direct assay:GO:0031369 translation
initiation factor binding
[PMID:9395514|PMID:22683627|PMID:15145951]"
/experiment="EXISTENCE:direct assay:GO:0043614 multi-eIF
complex [PMID:11018020|PMID:15838098]"
/experiment="EXISTENCE:direct assay:GO:1990856
methionyl-initiator methionine tRNA binding
[PMID:16565414]"
/experiment="EXISTENCE:mutant phenotype:GO:0001731
formation of translation preinitiation complex
[PMID:16449648]"
/experiment="EXISTENCE:mutant phenotype:GO:0003743
translation initiation factor activity [PMID:12137565]"
/experiment="EXISTENCE:mutant phenotype:GO:0005850
eukaryotic translation initiation factor 2 complex
[PMID:12137565]"
/experiment="EXISTENCE:physical interaction:GO:0031369
translation initiation factor binding
[PMID:15145951|PMID:22683627|PMID:9395514]"
/note="Beta subunit of the translation initiation factor
eIF2; involved in the identification of the start codon;
proposed to be involved in mRNA binding"
/codon_start=1
/product="translation initiation factor eIF2 subunit beta"
/protein_id="NP_015087.1"
/db_xref="GeneID:855838"
/db_xref="SGD:S000006158"
/translation="MSSDLAAELGFDPALKKKKKTKKVIPDDFDAAVNGKENGSGDDL
FAGLKKKKKKSKSVSADAEAEKEPTDDIAEALGELSLKKKKKKTKDSSVDAFEKELAK
AGLDNVDAESKEGTPSANSSIQQEVGLPYSELLSRFFNILRTNNPELAGDRSGPKFRI
PPPVCLRDGKKTIFSNIQDIAEKLHRSPEHLIQYLFAELGTSGSVDGQKRLVIKGKFQ
SKQMENVLRRYILEYVTCKTCKSINTELKREQSNRLFFMVCKSCGSTRSVSSIKTGFQ
ATVGKRRRM"
gene complement(<101608..>102702)
/gene="ENV7"
/locus_tag="YPL236C"
/db_xref="GeneID:855840"
mRNA complement(<101608..>102702)
/gene="ENV7"
/locus_tag="YPL236C"
/product="putative serine/threonine protein kinase ENV7"
/transcript_id="NM_001184050.1"
/db_xref="GeneID:855840"
CDS complement(101608..102702)
/gene="ENV7"
/locus_tag="YPL236C"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762|PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0006624
vacuolar protein processing [PMID:21912603]"
/experiment="EXISTENCE:mutant phenotype:GO:0032889
regulation of vacuole fusion, non-autophagic
[PMID:24610781]"
/note="Vacuolar membrane protein kinase; negatively
regulates membrane fusion; associates with vacuolar
membrane through palmitoylation of one or more cysteines
in consensus sequence; vacuolar membrane association is
essential to its kinase activity; targeted to vacuole via
AP-3 pathway; mutant shows defect in CPY processing;
ortholog of human serine/threonine kinase 16 (STK16)"
/codon_start=1
/product="putative serine/threonine protein kinase ENV7"
/protein_id="NP_015088.1"
/db_xref="GeneID:855840"
/db_xref="SGD:S000006157"
/translation="MISIVLELFQNLCCCRGFSDATIRVNDKRYRIQRLLGEGGMSFV
YLVQLSKNSLIIDNGIATPELYALKKIICPSVESISNGMREIENYKRFQSPYVIKSID
SQVMQEKDGSKTIYIVLPYYSLGSLQDSINRRLLEGTFVSEAECVRIMLGVTRGLLCL
HDPASRQDNATSRVNVDAVSMTYSDETAMLLEDTPLEMDMLSSNSAGSIAYAHRDITP
SNILFSSDGLPVIGDLGSCSQADITIENRHQLSELQEWVNDNCTLPYTPPELLNLKLN
QVLSSKVDIWSLGCTFYTLMFGISPFEREEQIHGASLTYAINTGKYSFPRNSRFSEGL
LSVIKKCIQVDPIQRPTTSQLLNLLQDLDT"
gene <103232..>104647
/gene="RVB2"
/locus_tag="YPL235W"
/gene_synonym="TIH2; TIP48; TIP49B"
/db_xref="GeneID:855841"
mRNA <103232..>104647
/gene="RVB2"
/locus_tag="YPL235W"
/gene_synonym="TIH2; TIP48; TIP49B"
/product="RuvB family ATP-dependent DNA helicase reptin"
/transcript_id="NM_001184049.1"
/db_xref="GeneID:855841"
CDS 103232..104647
/gene="RVB2"
/locus_tag="YPL235W"
/gene_synonym="TIH2; TIP48; TIP49B"
/EC_number="3.6.4.12"
/experiment="EXISTENCE:direct assay:GO:0000812 Swr1
complex [PMID:16299513]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11604509]"
/experiment="EXISTENCE:direct assay:GO:0043138 3'-5' DNA
helicase activity [PMID:20553504|PMID:10952318]"
/experiment="EXISTENCE:direct assay:GO:0043139 5'-3' DNA
helicase activity [PMID:18234224]"
/experiment="EXISTENCE:direct assay:GO:0097255 R2TP
complex [PMID:15766533]"
/experiment="EXISTENCE:direct assay:GO:0110078 TTT Hsp90
cochaperone complex [PMID:19040720]"
/experiment="EXISTENCE:mutant phenotype:GO:0000492 box C/D
snoRNP assembly [PMID:18268103|PMID:11604509]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:11278922]"
/experiment="EXISTENCE:mutant phenotype:GO:0006364 rRNA
processing [PMID:11604509]"
/experiment="EXISTENCE:physical interaction:GO:0000812
Swr1 complex [PMID:14690608|PMID:14645854]"
/experiment="EXISTENCE:physical interaction:GO:0006338
chromatin remodeling [PMID:14690608]"
/experiment="EXISTENCE:physical interaction:GO:0031011
Ino80 complex [PMID:24034245|PMID:10952318]"
/experiment="EXISTENCE:physical interaction:GO:0097255
R2TP complex [PMID:18268103]"
/note="ATP-dependent DNA helicase, also known as reptin;
conserved component of multiple complexes including the
INO80 complex, the Swr1 complex, the R2TP complex
(Rvb1-Rvb2-Tah1-Pih1) and the TTT Hsp90 cochaperone
complex that is involved in chromatin remodeling and
telomeric chromatin regulation; involved in multiple
processes such as chromatin remodeling, box C/D snoRNP
assembly, and RNA polymerase II assembly; member of the
AAA+ and RuvB protein families, similar to Rvb1p"
/codon_start=1
/product="RuvB family ATP-dependent DNA helicase reptin"
/protein_id="NP_015089.1"
/db_xref="GeneID:855841"
/db_xref="SGD:S000006156"
/translation="MSIQTSDPNETSDLKSLSLIAAHSHITGLGLDENLQPRPTSEGM
VGQLQARRAAGVILKMVQNGTIAGRAVLVAGPPSTGKTALAMGVSQSLGKDVPFTAIA
GSEIFSLELSKTEALTQAFRKSIGIKIKEETELIEGEVVEIQIDRSITGGHKQGKLTI
KTTDMETIYELGNKMIDGLTKEKVLAGDVISIDKASGKITKLGRSFARSRDYDAMGAD
TRFVQCPEGELQKRKTVVHTVSLHEIDVINSRTQGFLALFTGDTGEIRSEVRDQINTK
VAEWKEEGKAEIVPGVLFIDEVHMLDIECFSFINRALEDEFAPIVMMATNRGVSKTRG
TNYKSPHGLPLDLLDRSIIITTKSYNEQEIKTILSIRAQEEEVELSSDALDLLTKTGV
ETSLRYSSNLISVAQQIAMKRKNNTVEVEDVKRAYLLFLDSARSVKYVQENESQYIDD
QGNVQISIAKSADPDAMDTTE"
gene complement(<104946..>105440)
/gene="VMA11"
/locus_tag="YPL234C"
/gene_synonym="CLS9; TFP3"
/db_xref="GeneID:855842"
mRNA complement(<104946..>105440)
/gene="VMA11"
/locus_tag="YPL234C"
/gene_synonym="CLS9; TFP3"
/product="H(+)-transporting V0 sector ATPase subunit c'"
/transcript_id="NM_001184048.1"
/db_xref="GeneID:855842"
CDS complement(104946..105440)
/gene="VMA11"
/locus_tag="YPL234C"
/gene_synonym="CLS9; TFP3"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:11853670]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:15252052]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="Vacuolar ATPase V0 domain subunit c'; involved in
proton transport activity; hydrophobic integral membrane
protein (proteolipid) containing four transmembrane
segments; N and C termini are in the vacuolar lumen"
/codon_start=1
/product="H(+)-transporting V0 sector ATPase subunit c'"
/protein_id="NP_015090.1"
/db_xref="GeneID:855842"
/db_xref="SGD:S000006155"
/translation="MSTQLASNIYAPLYAPFFGFAGCAAAMVLSCLGAAIGTAKSGIG
IAGIGTFKPELIMKSLIPVVMSGILAIYGLVVAVLIAGNLSPTEDYTLFNGFMHLSCG
LCVGFACLSSGYAIGMVGDVGVRKYMHQPRLFVGIVLILIFSEVLGLYGMIVALILNT
RGSE"
gene <106172..>106822
/gene="NSL1"
/locus_tag="YPL233W"
/db_xref="GeneID:855843"
mRNA <106172..>106822
/gene="NSL1"
/locus_tag="YPL233W"
/product="MIND complex subunit NSL1"
/transcript_id="NM_001184047.1"
/db_xref="GeneID:855843"
CDS 106172..106822
/gene="NSL1"
/locus_tag="YPL233W"
/experiment="EXISTENCE:direct assay:GO:0000444 MIS12/MIND
type complex [PMID:14633972]"
/experiment="EXISTENCE:direct assay:GO:0000776 kinetochore
[PMID:14657030]"
/experiment="EXISTENCE:direct assay:GO:0000922 spindle
pole [PMID:12455957]"
/experiment="EXISTENCE:direct assay:GO:0007059 chromosome
segregation [PMID:12455957]"
/note="Essential component of the MIND kinetochore
complex; joins kinetochore subunits contacting DNA to
those contacting microtubules; required for accurate
chromosome segregation; complex consists of Mtw1p
Including Nnf1p-Nsl1p-Dsn1p (MIND)"
/codon_start=1
/product="MIND complex subunit NSL1"
/protein_id="NP_015091.1"
/db_xref="GeneID:855843"
/db_xref="SGD:S000006154"
/translation="MSQGQSKKLDVTVEQLRSIYHQFHDILEEKTDLHLPKKEYDDDA
VRREVQIQLQEFLLSAMTMASKSLEVVNADTVGKTVKQLIMESQEKYMEPFDLDLNEQ
VRKMYQEWEDETVKVAQLRQTGPAKINEVYNNSKDEYLAQLDGRIGVLQARMMQQQSA
DHDDSTDDADDHINWEHIKQDYVASLNELYQTQQDLPKVRYNVEKVKRLMDFLEED"
gene <107275..>108147
/gene="SSO1"
/locus_tag="YPL232W"
/db_xref="GeneID:855844"
mRNA <107275..>108147
/gene="SSO1"
/locus_tag="YPL232W"
/product="syntaxin"
/transcript_id="NM_001184046.1"
/db_xref="GeneID:855844"
CDS 107275..108147
/gene="SSO1"
/locus_tag="YPL232W"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:19502581]"
/experiment="EXISTENCE:direct assay:GO:0005484 SNAP
receptor activity [PMID:16339720]"
/experiment="EXISTENCE:direct assay:GO:0005546
phosphatidylinositol-4,5-bisphosphate binding
[PMID:19502581]"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:17298976|PMID:19502581]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:17298976|PMID:19502581|PMID:16622836]"
/experiment="EXISTENCE:direct assay:GO:0006906 vesicle
fusion [PMID:11001046]"
/experiment="EXISTENCE:direct assay:GO:0031201 SNARE
complex [PMID:9195974]"
/experiment="EXISTENCE:direct assay:GO:0043325
phosphatidylinositol-3,4-bisphosphate binding
[PMID:19502581]"
/experiment="EXISTENCE:direct assay:GO:0070300
phosphatidic acid binding [PMID:19502581]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0080025
phosphatidylinositol-3,5-bisphosphate binding
[PMID:19502581]"
/experiment="EXISTENCE:genetic interaction:GO:0031321
ascospore-type prospore assembly [PMID:19502581]"
/experiment="EXISTENCE:mutant phenotype:GO:0030437
ascospore formation [PMID:11839791]"
/experiment="EXISTENCE:mutant phenotype:GO:0031321
ascospore-type prospore assembly
[PMID:16554438|PMID:19502581]"
/experiment="EXISTENCE:physical interaction:GO:0005484
SNAP receptor activity [PMID:7954793|PMID:10924463]"
/note="Plasma membrane t-SNARE; involved in fusion of
secretory vesicles at the plasma membrane and in vesicle
fusion during sporulation; forms a complex with Sec9p that
binds v-SNARE Snc2p; syntaxin homolog; functionally
redundant with Sso2p; SSO1 has a paralog, SSO2, that arose
from the whole genome duplication"
/codon_start=1
/product="syntaxin"
/protein_id="NP_015092.1"
/db_xref="GeneID:855844"
/db_xref="SGD:S000006153"
/translation="MSYNNPYQLETPFEESYELDEGSSAIGAEGHDFVGFMNKISQIN
RDLDKYDHTINQVDSLHKRLLTEVNEEQASHLRHSLDNFVAQATDLQFKLKNEIKSAQ
RDGIHDTNKQAQAENSRQRFLKLIQDYRIVDSNYKEENKEQAKRQYMIIQPEATEDEV
EAAISDVGGQQIFSQALLNANRRGEAKTALAEVQARHQELLKLEKSMAELTQLFNDME
ELVIEQQENVDVIDKNVEDAQLDVEQGVGHTDKAVKSARKARKNKIRCWLIVFAIIVV
VVVVVVVPAVVKTR"
gene <108652..>114315
/gene="FAS2"
/locus_tag="YPL231W"
/db_xref="GeneID:855845"
mRNA <108652..>114315
/gene="FAS2"
/locus_tag="YPL231W"
/product="trifunctional fatty acid synthase subunit FAS2"
/transcript_id="NM_001184045.1"
/db_xref="GeneID:855845"
CDS 108652..114315
/gene="FAS2"
/locus_tag="YPL231W"
/EC_number="1.1.1.100"
/EC_number="2.3.1.41"
/EC_number="2.3.1.86"
/experiment="EXISTENCE:direct assay:GO:0004316
3-oxoacyl-[acyl-carrier-protein] reductase (NADPH)
activity [PMID:365179]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762|PMID:6025308]"
/experiment="EXISTENCE:direct assay:GO:0005835 fatty acid
synthase complex [PMID:65153]"
/experiment="EXISTENCE:mutant phenotype:GO:0004312 fatty
acid synthase activity [PMID:65153]"
/experiment="EXISTENCE:mutant phenotype:GO:0004315
3-oxoacyl-[acyl-carrier-protein] synthase activity
[PMID:1100391]"
/experiment="EXISTENCE:mutant phenotype:GO:0008897
holo-[acyl-carrier-protein] synthase activity
[PMID:10785388]"
/experiment="EXISTENCE:mutant phenotype:GO:0042759
long-chain fatty acid biosynthetic process [PMID:1735446]"
/note="Alpha subunit of fatty acid synthetase (FAS); the
FAS complex catalyzes the synthesis of long-chain
saturated fatty acids and is subject to allosteric
regulation by palmitoyl-CoA binding distal to the
catalytic site to inhibit ketoacyl reductase activity;
contains the acyl-carrier protein domain and beta-ketoacyl
reductase, beta-ketoacyl synthase and
self-pantetheinylation activities"
/codon_start=1
/product="trifunctional fatty acid synthase subunit FAS2"
/protein_id="NP_015093.1"
/db_xref="GeneID:855845"
/db_xref="SGD:S000006152"
/translation="MKPEVEQELAHILLTELLAYQFASPVRWIETQDVFLKDFNTERV
VEIGPSPTLAGMAQRTLKNKYESYDAALSLHREILCYSKDAKEIYYTPDPSELAAKEE
PAKEEAPAPTPAASAPAPAAAAPAPVAAAAPAAAAAEIADEPVKASLLLHVLVAHKLK
KSLDSIPMSKTIKDLVGGKSTVQNEILGDLGKEFGTTPEKPEETPLEELAETFQDTFS
GALGKQSSSLLSRLISSKMPGGFTITVARKYLQTRWGLPSGRQDGVLLVALSNEPAAR
LGSEADAKAFLDSMAQKYASIVGVDLSSAASASGAAGAGAAAGAAMIDAGALEEITKD
HKVLARQQLQVLARYLKMDLDNGERKFLKEKDTVAELQAQLDYLNAELGEFFVNGVAT
SFSRKKARTFDSSWNWAKQSLLSLYFEIIHGVLKNVDREVVSEAINIMNRSNDALIKF
MEYHISNTDETKGENYQLVKTLGEQLIENCKQVLDVDPVYKDVAKPTGPKTAIDKNGN
ITYSEEPREKVRKLSQYVQEMALGGPITKESQPTIEEDLTRVYKAISAQADKQDISSS
TRVEFEKLYSDLMKFLESSKEIDPSQTTQLAGMDVEDALDKDSTKEVASLPNKSTISK
TVSSTIPRETIPFLHLRKKTPAGDWKYDRQLSSLFLDGLEKAAFNGVTFKDKYVLITG
AGKGSIGAEVLQGLLQGGAKVVVTTSRFSKQVTDYYQSIYAKYGAKGSTLIVVPFNQG
SKQDVEALIEFIYDTEKNGGLGWDLDAIIPFAAIPEQGIELEHIDSKSEFAHRIMLTN
ILRMMGCVKKQKSARGIETRPAQVILPMSPNHGTFGGDGMYSESKLSLETLFNRWHSE
SWANQLTVCGAIIGWTRGTGLMSANNIIAEGIEKMGVRTFSQKEMAFNLLGLLTPEVV
ELCQKSPVMADLNGGLQFVPELKEFTAKLRKELVETSEVRKAVSIETALEHKVVNGNS
ADAAYAQVEIQPRANIQLDFPELKPYKQVKQIAPAELEGLLDLERVIVVTGFAEVGPW
GSARTRWEMEAFGEFSLEGCVEMAWIMGFISYHNGNLKGRPYTGWVDSKTKEPVDDKD
VKAKYETSILEHSGIRLIEPELFNGYNPEKKEMIQEVIVEEDLEPFEASKETAEQFKH
QHGDKVDIFEIPETGEYSVKLLKGATLYIPKALRFDRLVAGQIPTGWNAKTYGISDDI
ISQVDPITLFVLVSVVEAFIASGITDPYEMYKYVHVSEVGNCSGSGMGGVSALRGMFK
DRFKDEPVQNDILQESFINTMSAWVNMLLISSSGPIKTPVGACATSVESVDIGVETIL
SGKARICIVGGYDDFQEEGSFEFGNMKATSNTLEEFEHGRTPAEMSRPATTTRNGFME
AQGAGIQIIMQADLALKMGVPIYGIVAMAATATDKIGRSVPAPGKGILTTAREHHSSV
KYASPNLNMKYRKRQLVTREAQIKDWVENELEALKLEAEEIPSEDQNEFLLERTREIH
NEAESQLRAAQQQWGNDFYKRDPRIAPLRGALATYGLTIDDLGVASFHGTSTKANDKN
ESATINEMMKHLGRSEGNPVIGVFQKFLTGHPKGAAGAWMMNGALQILNSGIIPGNRN
ADNVDKILEQFEYVLYPSKTLKTDGVRAVSITSFGFGQKGGQAIVVHPDYLYGAITED
RYNEYVAKVSAREKSAYKFFHNGMIYNKLFVSKEHAPYTDELEEDVYLDPLARVSKDK
KSGSLTFNSKNIQSKDSYINANTIETAKMIENMTKEKVSNGGVGVDVELITSINVEND
TFIERNFTPQEIEYCSAQPSVQSSFAGTWSAKEAVFKSLGVKSLGGGAALKDIEIVRV
NKNAPAVELHGNAKKAAEEAGVTDVKVSISHDDLQAVAVAVSTKK"
gene <115312..>116487
/gene="USV1"
/locus_tag="YPL230W"
/gene_synonym="NSF1"
/db_xref="GeneID:855871"
mRNA <115312..>116487
/gene="USV1"
/locus_tag="YPL230W"
/gene_synonym="NSF1"
/product="Usv1p"
/transcript_id="NM_001184044.1"
/db_xref="GeneID:855871"
CDS 115312..116487
/gene="USV1"
/locus_tag="YPL230W"
/gene_synonym="NSF1"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:18667581|PMID:27663510]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding
[PMID:19111667|PMID:19158363]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:18667581]"
/experiment="EXISTENCE:mutant phenotype:GO:0071472
cellular response to salt stress [PMID:18667581]"
/note="Putative transcription factor containing a C2H2
zinc finger; mutation affects transcriptional regulation
of genes involved in growth on non-fermentable carbon
sources, response to salt stress and cell wall
biosynthesis; USV1 has a paralog, RGM1, that arose from
the whole genome duplication"
/codon_start=1
/product="Usv1p"
/protein_id="NP_015094.1"
/db_xref="GeneID:855871"
/db_xref="SGD:S000006151"
/translation="MENTTNRNTAGVLTSSNGNFATNSVAASTPKRSKSARRKTFKCT
GYDGCTMSFTRAEHLARHIRKHTGEKPFQCPACLKFFSRVDNLKQHRESVHAHKNHHS
TSSHQRKPSSSSLSSSSSASSSSSASSSTSYSDPYRKTNINSGNMPMMAENEKAPQII
HSSPEFITSTRSIPPISPRSIYNTQRQQQHQQQQHQQAPYYFPSHPITDSYYQYPLPS
NNNTINYLPSVDVQYPLNVSPSSTSHPASEVIISSFPPRSMPSTSFKYKDSADFQART
TMNKYNIRPSNINVNTSNINNHLDSFSPPFSPSTTVAEAKPIILPQYQQAFSQPPNGN
KNNNMSSSKNGGKGGENFKNTDDRNDNNNKKRSETLSESDISVNTNKKRLSVDYILT"
rep_origin 116566..116680
/note="ARS1607; Autonomously Replicating Sequence"
/db_xref="SGD:S000118413"
gene <117067..>117687
/locus_tag="YPL229W"
/db_xref="GeneID:855872"
mRNA <117067..>117687
/locus_tag="YPL229W"
/product="uncharacterized protein"
/transcript_id="NM_001184043.1"
/db_xref="GeneID:855872"
CDS 117067..117687
/locus_tag="YPL229W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to the cytoplasm; not an
essential gene; YPL229W has a paralog, YMR181C, that arose
from the whole genome duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015095.1"
/db_xref="GeneID:855872"
/db_xref="SGD:S000006150"
/translation="MMPYNTPPNIQEPMNFASSNPFGIIPDALSFQNFKYDRLQQQQQ
QQQQQQQNRTASSLQQPQQQQPISPPLFLVGAGTSENSNLNKNANTSTIPPLLFSRSS
QHYVVPDIDHSSIIYKNNICKSFKDDLFFCPRSLLSLEEQQACEKMDRLTAEQMSLYH
QNTQSSSNPGSMSSSPPNSASSIFNSRPKFNPYTSQSFNPLESVQE"
gene <118382..>120031
/gene="CET1"
/locus_tag="YPL228W"
/gene_synonym="CES5"
/db_xref="GeneID:855873"
mRNA <118382..>120031
/gene="CET1"
/locus_tag="YPL228W"
/gene_synonym="CES5"
/product="polynucleotide 5'-phosphatase"
/transcript_id="NM_001184042.1"
/db_xref="GeneID:855873"
CDS 118382..120031
/gene="CET1"
/locus_tag="YPL228W"
/gene_synonym="CES5"
/EC_number="3.6.1.74"
/experiment="EXISTENCE:direct assay:GO:0004651
polynucleotide 5'-phosphatase activity
[PMID:9345280|PMID:17707331]"
/experiment="EXISTENCE:genetic interaction:GO:0031533 mRNA
capping enzyme complex [PMID:9710603]"
/experiment="EXISTENCE:mutant phenotype:GO:0032968
positive regulation of transcription elongation by RNA
polymerase II [PMID:24172134]"
/experiment="EXISTENCE:mutant phenotype:GO:1900182
positive regulation of protein localization to nucleus
[PMID:24205062]"
/experiment="EXISTENCE:physical interaction:GO:0006370
7-methylguanosine mRNA capping [PMID:9345280]"
/experiment="EXISTENCE:physical interaction:GO:0031533
mRNA capping enzyme complex [PMID:9710603]"
/note="RNA 5'-triphosphatase involved in mRNA 5' capping;
subunit of mRNA capping enzyme, which is a heterotetramer
composed of a Cet1p homodimer and two molecules of
guanylyltransferase Ceg1p; Cet1p also has a role in
regulation of RNAPII pausing at promoter-proximal sites;
interaction between Cet1p and Ceg1p is required for Ceg1p
nuclear import; mammalian enzyme is single bifunctional
polypeptide; human homolog RNGTT can complement yeast cet1
null mutant"
/codon_start=1
/product="polynucleotide 5'-phosphatase"
/protein_id="NP_015096.1"
/db_xref="GeneID:855873"
/db_xref="SGD:S000006149"
/translation="MSYTDNPPQTKRALSLDDLVNHDENEKVKLQKLSEAANGSRPFA
ENLESDINQTETGQAAPIDNYKESTGHGSHSQKPKSRKSSNDDEETDTDDEMGASGEI
NFDSEMDFDYDKQHRNLLSNGSPPMNDGSDANAKLEKPSDDSIHQNSKSDEEQRIPKQ
GNEGNIASNYITQVPLQKQKQTEKKIAGNAVGSVVKKEEEANAAVDNIFEEKATLQSK
KNNIKRDLEVLNEISASSKPSKYRNVPIWAQKWKPTIKALQSINVKDLKIDPSFLNII
PDDDLTKSVQDWVYATIYSIAPELRSFIELEMKFGVIIDAKGPDRVNPPVSSQCVFTE
LDAHLTPNIDASLFKELSKYIRGISEVTENTGKFSIIESQTRDSVYRVGLSTQRPRFL
RMSTDIKTGRVGQFIEKRHVAQLLLYSPKDSYDVKISLNLELPVPDNDPPEKYKSQSP
ISERTKDRVSYIHNDSCTRIDITKVENHNQNSKSRQSETTHEVELEINTPALLNAFDN
ITNDSKEYASLIRTFLNNGTIIRRKLSSLSYEIFEGSKKVM"
gene complement(<120163..>121167)
/gene="ALG5"
/locus_tag="YPL227C"
/db_xref="GeneID:855874"
mRNA complement(<120163..>121167)
/gene="ALG5"
/locus_tag="YPL227C"
/product="dolichyl-phosphate beta-glucosyltransferase"
/transcript_id="NM_001184041.1"
/db_xref="GeneID:855874"
CDS complement(120163..121167)
/gene="ALG5"
/locus_tag="YPL227C"
/EC_number="2.4.1.117"
/experiment="EXISTENCE:direct assay:GO:0004581
dolichyl-phosphate beta-glucosyltransferase activity
[PMID:8076653]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:9560251]"
/note="UDP-glucose:dolichyl-phosphate glucosyltransferase;
involved in asparagine-linked glycosylation in the
endoplasmic reticulum; human ortholog ALG5 can partially
complement yeast alg5 mutant"
/codon_start=1
/product="dolichyl-phosphate beta-glucosyltransferase"
/protein_id="NP_015097.1"
/db_xref="GeneID:855874"
/db_xref="SGD:S000006148"
/translation="MRALRFLIENRNTVFFTLLVALVLSLYLLVYLFSHTPRPPYPEE
LKYIAIDEKGHEVSRALPNLNEHQDDEEIFLSVVIPSYNETGRILLMLTDAISFLKEK
YGSRWEIVIVDDGSTDNTTQYCLKICKEQFKLNYEQFRIIKFSQNRGKGGAVRQGFLH
IRGKYGLFADADGASKFSDVEKLIDAISKIETSSTDLKTTKPAVAIGSRAHMVNTEAV
IKRSMIRNCLMYGFHTLVFIFGIRSIKDTQCGFKLFNRAAILKIFPYLHTEGWIFDVE
ILILAIRKRIQIEEIPISWHEVDGSKMALAIDSIKMAKDLVIIRMAYLLGIYRDNKKC
"
gene <121767..>125357
/gene="NEW1"
/locus_tag="YPL226W"
/db_xref="GeneID:855875"
mRNA <121767..>125357
/gene="NEW1"
/locus_tag="YPL226W"
/product="New1p"
/transcript_id="NM_001184040.1"
/db_xref="GeneID:855875"
CDS 121767..125357
/gene="NEW1"
/locus_tag="YPL226W"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:23222640|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:31299085]"
/experiment="EXISTENCE:mutant phenotype:GO:0006449
regulation of translational termination [PMID:31299085]"
/experiment="EXISTENCE:mutant phenotype:GO:0008079
translation termination factor activity [PMID:31299085]"
/experiment="EXISTENCE:mutant phenotype:GO:0042274
ribosomal small subunit biogenesis [PMID:19806183]"
/note="Translation termination and ribosome biogenesis
factor; ATP binding cassette protein that cosediments with
polysomes and is required for biogenesis of the small
ribosomal subunit; Asn/Gln-rich rich region supports [NU+]
prion formation and susceptibility to [PSI+] prion
induction"
/codon_start=1
/product="New1p"
/protein_id="NP_015098.1"
/db_xref="GeneID:855875"
/db_xref="SGD:S000006147"
/translation="MPPKKFKDLNSFLDDQPKDPNLVASPFGGYFKNPAADAGSNNAS
KKSSYQQQRNWKQGGNYQQGGYQSYNSNYNNYNNYNNYNNYNNYNNYNKYNGQGYQKS
TYKQSAVTPNQSGTPTPSASTTSLTSLNEKLSNLELTPISQFLSKIPECQSITDCKNQ
IKLIIEEFGKEGNSTGEKIEEWKIVDVLSKFIKPKNPSLVRESAMLIISNIAQFFSGK
PPQEAYLLPFFNVALDCISDKENTVKRAAQHAIDSLLNCFPMEALTCFVLPTILDYLS
SGAKWQAKMAALSVVDRIREDSANDLLELTFKDAVPVLTDVATDFKPELAKQGYKTLL
DYVSILDNLDLSPRYKLIVDTLQDPSKVPESVKSLSSVTFVAEVTEPSLSLLVPILNR
SLNLSSSSQEQLRQTVIVVENLTRLVNNRNEIESFIPLLLPGIQKVVDTASLPEVREL
AEKALNVLKEDDEADKENKFSGRLTLEEGRDFLLDHLKDIKADDSCFVKPYMNDETVI
KYMSKILTVDSNVNDWKRLEDFLTAVFGGSDSQREFVKQDFIHNLRALFYQEKERADE
DEGIEIVNTDFSLAYGSRMLLNKTNLRLLKGHRYGLCGRNGAGKSTLMRAIANGQLDG
FPDKDTLRTCFVEHKLQGEEGDLDLVSFIALDEELQSTSREEIAAALESVGFDEERRA
QTVGSLSGGWKMKLELARAMLQKADILLLDEPTNHLDVSNVKWLEEYLLEHTDITSLI
VSHDSGFLDTVCTDIIHYENKKLAYYKGNLAAFVEQKPEAKSYYTLTDSNAQMRFPPP
GILTGVKSNTRAVAKMTDVTFSYPGAQKPSLSHVSCSLSLSSRVACLGPNGAGKSTLI
KLLTGELVPNEGKVEKHPNLRIGYIAQHALQHVNEHKEKTANQYLQWRYQFGDDREVL
LKESRKISEDEKEMMTKEIDIDDGRGKRAIEAIVGRQKLKKSFQYEVKWKYWKPKYNS
WVPKDVLVEHGFEKLVQKFDDHEASREGLGYRELIPSVITKHFEDVGLDSEIANHTPL
GSLSGGQLVKVVIAGAMWNNPHLLVLDEPTNYLDRDSLGALAVAIRDWSGGVVMISHN
NEFVGALCPEQWIVENGKMVQKGSAQVDQSKFEDGGNADAVGLKASNLAKPSVDDDDS
PANIKVKQRKKRLTRNEKKLQAERRRLRYIEWLSSPKGTPKPVDTDDEED"
gene <126006..>126446
/gene="CHP1"
/locus_tag="YPL225W"
/db_xref="GeneID:855876"
mRNA <126006..>126446
/gene="CHP1"
/locus_tag="YPL225W"
/product="ribosome-associated Tef1p biogenesis chaperone
CHP1"
/transcript_id="NM_001184039.1"
/db_xref="GeneID:855876"
CDS 126006..126446
/gene="CHP1"
/locus_tag="YPL225W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:38360885]"
/experiment="EXISTENCE:direct assay:GO:0044183 protein
folding chaperone [PMID:38360885]"
/experiment="EXISTENCE:direct assay:GO:0051083 'de novo'
cotranslational protein folding [PMID:38360885]"
/experiment="EXISTENCE:direct assay:GO:0051787 misfolded
protein binding [PMID:38360885]"
/experiment="EXISTENCE:direct assay:GO:0061770 translation
elongation factor binding [PMID:38360885]"
/experiment="EXISTENCE:direct assay:GO:1990593 nascent
polypeptide-associated complex binding [PMID:38360885]"
/note="Chaperone for eEF1A; ribosome-associated chaperone
dedicated to facilitating high-level expression of eEF1A;
transiently binds nascent GTPase domain of eEF1A as soon
as it emerges from ribosome tunnel; forms complex with NAC
by binding UBA domain of Egd2p; inactivation results in
disturbed proteostasis and Hsf1p activation due to
abberant eEF1A biogenesis; overlapping phenotypes support
notion that Chp1p functions in same eEF1A biogenesis
pathway as chaperone Zpr1p-Aim29p"
/codon_start=1
/product="ribosome-associated Tef1p biogenesis chaperone
CHP1"
/protein_id="NP_015099.1"
/db_xref="GeneID:855876"
/db_xref="SGD:S000006146"
/translation="MSTFNAETADNLEDIEKQFAVVAVEQAETYWKLLTSVPGSKLRL
TKFDDEIYENFMERFPEYKDVERVKKFTEEELKTKEAKERWRKFFTIFEKKIEDYNFG
TLLRTDASAEYGQFTTCFVVRLQFYAFEIARNKHGLNDWIVGQK"
gene complement(<126634..>128088)
/gene="MMT2"
/locus_tag="YPL224C"
/gene_synonym="MFT2"
/db_xref="GeneID:855877"
mRNA complement(<126634..>128088)
/gene="MMT2"
/locus_tag="YPL224C"
/gene_synonym="MFT2"
/product="Mmt2p"
/transcript_id="NM_001184038.2"
/db_xref="GeneID:855877"
CDS complement(126634..128088)
/gene="MMT2"
/locus_tag="YPL224C"
/gene_synonym="MFT2"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:16823961|PMID:14576278|PMID:9353309|PMID:24769239]"
/experiment="EXISTENCE:genetic interaction:GO:0006879
intracellular iron ion homeostasis [PMID:9353309]"
/experiment="EXISTENCE:mutant phenotype:GO:0006879
intracellular iron ion homeostasis [PMID:9353309]"
/note="Putative metal transporter involved in
mitochondrial iron accumulation; MMT2 has a paralog, MMT1,
that arose from the whole genome duplication"
/codon_start=1
/product="Mmt2p"
/protein_id="NP_015100.2"
/db_xref="GeneID:855877"
/db_xref="SGD:S000006145"
/translation="MLRISIDSIKQFGSFVPGYNNTSYHAAGRAIRTSSLYSTMISAN
PRRCLHSSKLLNKEGQEEGYNEQLISKMSSQNGSNSRQNESEGKKEGKASSVKSLLQH
THSHSHTHMHDNPLLSLNVQQIKKNPGVRITWIGLASNVGMAVGKFVGGITFHSQALL
ADSVHALSDLVSDFLTLFSVQYASRKPTSEYPYGYGKVETVGSLAVSTILAMAGISIG
WSSLCAIVGPVIPHAILESMAGLIGETHSHSQSLTQQATNVNAVWIAAGSILVKEWVF
QATKKVAIQTNSNVLMANAWHHRVDSLTSLVALVAITSSYFFNIQSLDNLGGLVVSGL
IIKTGGQGILSSLKELVDQSIPPTDPRYLEIESVIKDSIGSLKTDLDLKQSLHVRDLT
ILASGPNLRATTTLEVPVLHSGQEVGIRFLENAISTIREDLRMKVPNVGKVDVEFVDV
TSDSKGDLEHSHDTKSTNHTHTHSDSADTHTHKH"
gene complement(<128632..>129138)
/gene="GRE1"
/locus_tag="YPL223C"
/db_xref="GeneID:855878"
mRNA complement(<128632..>129138)
/gene="GRE1"
/locus_tag="YPL223C"
/product="Gre1p"
/transcript_id="NM_001184037.1"
/db_xref="GeneID:855878"
CDS complement(128632..129138)
/gene="GRE1"
/locus_tag="YPL223C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276|PMID:14562095]"
/note="Hydrophilin essential in desiccation-rehydration
process; stress induced (osmotic, ionic, oxidative, heat
shock and heavy metals); regulated by the HOG pathway;
GRE1 has a paralog, SIP18, that arose from the whole
genome duplication"
/codon_start=1
/product="Gre1p"
/protein_id="NP_015101.1"
/db_xref="GeneID:855878"
/db_xref="SGD:S000006144"
/translation="MSNLLNKFADKLHGNDHDERYEDDNDDQTRQQRHEKHQQREFRN
QGSKADPYGEENQGNFPQRQQPQSNLGGNTQFGGNDFQQQTTDYTAGTGGGTYTQTYR
ETNTQGQLDDDEDDDFLTSGQQQKQGRTRGAQSNRYQSSNIGSGRRDLSGSGNDEYDD
DSGNQGVW"
gene <130162..>132228
/gene="FMP40"
/locus_tag="YPL222W"
/db_xref="GeneID:855879"
mRNA <130162..>132228
/gene="FMP40"
/locus_tag="YPL222W"
/product="Fmp40p"
/transcript_id="NM_001184036.1"
/db_xref="GeneID:855879"
CDS 130162..132228
/gene="FMP40"
/locus_tag="YPL222W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:30270044|PMID:24769239|PMID:14576278|PMID:16823961|P
MID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0018117 protein
adenylylation [PMID:30270044]"
/experiment="EXISTENCE:direct assay:GO:0045454 cell redox
homeostasis [PMID:30270044]"
/experiment="EXISTENCE:direct assay:GO:0070733 AMPylase
activity [PMID:30270044]"
/note="Mitochondrial AMPylator in the pseudokinase
selenoprotein-O family; role in the oxidative stress
response, transferring AMP from ATP to protein substrates
(AMPylation) involved in redox homeostasis; regulates
S-glutathionylation levels; proposed involvement in the
environmental stress response"
/codon_start=1
/product="Fmp40p"
/protein_id="NP_015102.1"
/db_xref="GeneID:855879"
/db_xref="SGD:S000006143"
/translation="MGEKRTIIKALKNSAASHFIKKLTADTSLSSIQEAINVVQQYNA
TDPVRLKLFHTPRMVSQGAHFAFCLPTKKPHYKPLLLSQNALDEFNLVQDQDLEKILS
GEKVYYSDSIFPYSTVYSGFQFGSFAAQLGDGRVVNLFDLKDKCSGQWQTFQLKGAGM
TPFSRFADGKAVLRSSIREFIMSEALHSIGIPSTRAMQLTLLPGTKAQRRNQEPCAVV
CRFAPSWIRLGNFNLFRWRHDLKGLIQLSDYCIEELFAGGTQFEGKPDFNIFKRDFFP
DTETKIDEQVEKDETEVSTMTGDNISTLSKYDEFFRHVVSLNANTVAHWQAYGFANGV
LNTDNTSIMGLTIDYGPFAFLDKFEPSFTPNHDDTAKRYSFANQPSIIWWNLQQFAKD
LACLLGPEARDLELLLKGELNSVDDALEKTMIERVQKLVELSANEYKYVFTTRYAQIM
SQRLGVDLDLEKCMSSTNLKDIEHAAEKAKEFCDVIVEPLLDILQATKVDYNNFFIHL
QNYKGPFFIKDKSDTATLFGAFDEEYLGMFFNSKQLQQMAETEEAFAAGEKVFDANGE
LRLLNEKLQEIRNWTQDYLTLVPPTETAARASLAKKANPLFVPRSWVLEEVVDDLMYS
QRDGLQDPSSELDTSALKKLYLMSVNPYDRTKWDVTLRPELETKWADLSHQDDAKFMM
QASCSS"
gene <133043..>135424
/gene="FLC1"
/locus_tag="YPL221W"
/db_xref="GeneID:855880"
mRNA <133043..>135424
/gene="FLC1"
/locus_tag="YPL221W"
/product="flavin adenine dinucleotide transporter"
/transcript_id="NM_001184035.1"
/db_xref="GeneID:855880"
CDS 133043..135424
/gene="FLC1"
/locus_tag="YPL221W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:16717099]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0030148
sphingolipid biosynthetic process [PMID:27462707]"
/experiment="EXISTENCE:mutant phenotype:GO:0006457 protein
folding [PMID:16717099]"
/experiment="EXISTENCE:mutant phenotype:GO:0009272
fungal-type cell wall biogenesis [PMID:16717099]"
/experiment="EXISTENCE:mutant phenotype:GO:0015230 FAD
transmembrane transporter activity [PMID:16717099]"
/experiment="EXISTENCE:mutant phenotype:GO:0015883 FAD
transport [PMID:16717099]"
/note="Flavin adenine dinucleotide transporter; required
for uptake of FAD into endoplasmic reticulum; involved in
cell wall maintenance; FLC1 has a paralog, FLC3, that
arose from the whole genome duplication"
/codon_start=1
/product="flavin adenine dinucleotide transporter"
/protein_id="NP_015103.1"
/db_xref="GeneID:855880"
/db_xref="SGD:S000006142"
/translation="MQVLVTLWCLICTCLVLPVAAKKRTLTASSLVTCMENSQLSANS
FDVSFSPDDRSLHYDLDMTTQIDSYIYAYVDVYAYGFKIITENFDVCSMGWKQFCPVH
PGNIQIDSIEYIAQKYVKMIPGIAYQVPDIDAYVRLNIYNNVSENLACIQVFFSNGKT
VSQIGVKWVTAVIAGIGLLTSAVLSTFGNSTAASHISANTMSLFLYFQSVAVVAMQHV
DSVPPIAAAWSENLAWSMGLIRITFMQKIFRWYVEATGGSASLYLTATTMSVLTQRGL
DYLKNTSVYKRAENVLYGNSNTLIFRGIKRMGYRMKIENTAIVCTGFTFFVLCGYFLA
GFIMACKYSIELCIRCGWMRSDRFYQFRKNWRSVLKGSLLRYIYIGFTQLTILSFWEF
TERDSAGVIVIACLFIVLSCGLMAWAAYRTIFFASKSVEMYNNPAALLYGDEYVLNKY
GFFYTMFNAKHYWWNALLTTYILVKALFVGFAQASGKTQALAIFIIDLAYFVAIIRYK
PYLDRPTNIVNIFICTVTLVNSFLFMFFSNLFNQKYAVSAIMGWVFFIMNAAFSLLLL
LMILAFTTIILFSKNPDSRFKPAKDDRASFQKHAIPHEGALNKSVANELMALGNVAKD
HTENWEYELKSQEGKSEDNLFGVEYDDEKTGTNSENAESSSKETTRPTFSEKVLRSLS
IKRNKSKLGSFKRSAPDKITQQEVSPDRASSSPNSKSYPGVSHTRQESEANNGLINAY
EDEQFSLMEPSILEDAASSTQMHAMPARDLSLSSVANAQDVTKKANILDPDYL"
gene <135790..>136443
/gene="RPL1A"
/locus_tag="YPL220W"
/gene_synonym="PUB2; SSM1"
/db_xref="GeneID:855881"
mRNA <135790..>136443
/gene="RPL1A"
/locus_tag="YPL220W"
/gene_synonym="PUB2; SSM1"
/product="60S ribosomal protein uL1 RPL1A"
/transcript_id="NM_001184034.1"
/db_xref="GeneID:855881"
CDS 135790..136443
/gene="RPL1A"
/locus_tag="YPL220W"
/gene_synonym="PUB2; SSM1"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:11983894]"
/experiment="EXISTENCE:curator inference:GO:0003735
structural constituent of ribosome [PMID:11983894]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:11983894]"
/experiment="EXISTENCE:mutant phenotype:GO:0000055
ribosomal large subunit export from nucleus
[PMID:31439809]"
/note="Ribosomal 60S subunit protein L1A; N-terminally
acetylated; homologous to mammalian ribosomal protein L10A
and bacterial L1; RPL1A has a paralog, RPL1B, that arose
from the whole genome duplication; rpl1a rpl1b double null
mutation is lethal; ribosomes containing Rpl1b are more
efficient in translation of respiration-related proteins"
/codon_start=1
/product="60S ribosomal protein uL1 RPL1A"
/protein_id="NP_015104.1"
/db_xref="GeneID:855881"
/db_xref="SGD:S000006141"
/translation="MSKITSSQVREHVKELLKYSNETKKRNFLETVELQVGLKNYDPQ
RDKRFSGSLKLPNCPRPNMSICIFGDAFDVDRAKSCGVDAMSVDDLKKLNKNKKLIKK
LSKKYNAFIASEVLIKQVPRLLGPQLSKAGKFPTPVSHNDDLYGKVTDVRSTIKFQLK
KVLCLAVAVGNVEMEEDVLVNQILMSVNFFVSLLKKNWQNVGSLVVKSSMGPAFRLY"
gene <136750..>138228
/gene="PCL8"
/locus_tag="YPL219W"
/db_xref="GeneID:855882"
mRNA <136750..>138228
/gene="PCL8"
/locus_tag="YPL219W"
/product="Pcl8p"
/transcript_id="NM_001184033.1"
/db_xref="GeneID:855882"
CDS 136750..138228
/gene="PCL8"
/locus_tag="YPL219W"
/experiment="EXISTENCE:genetic interaction:GO:0045719
negative regulation of glycogen biosynthetic process
[PMID:9584169]"
/experiment="EXISTENCE:mutant phenotype:GO:0045719
negative regulation of glycogen biosynthetic process
[PMID:9584169]"
/note="Cyclin; interacts with Pho85p cyclin-dependent
kinase (Cdk) to phosphorylate and regulate glycogen
synthase, also activates Pho85p for Glc8p phosphorylation;
PCL8 has a paralog, PCL10, that arose from the whole
genome duplication"
/codon_start=1
/product="Pcl8p"
/protein_id="NP_015105.1"
/db_xref="GeneID:855882"
/db_xref="SGD:S000006140"
/translation="MANDQDPNKSLINDALTRSMSEFYDDDDDNDSDMCRANDEGEDV
FDLPLKVGVSQSRNFSEVNDVLDPLSSLHGPSKKVRFEQQKQQQQHQQLHNDFNTDFN
LKSPSSKKMGVEQLIQSANEINDYLANNIDKVNSFNSELLSGSGKLPGRVKSDTATQG
TGRLDSMSNFALSDTELDNDDDNYLLDPLANASSTTPTVEHHGYSLLDKALSTSDKEK
IYTNKVNSNSQIDTDNHSHESGNTTNNETDENESSEILDYTKFDSFPYPPSSAPNGEP
PDLKVLSIECEQENEKELRRISLLLDHYESIPKIPELSDDEALSKFRENIELILQLSK
KINDNANTLAISSEDPQKFVNFVMKNPPSLSFRDFIDRIQNKCMFGAVVYLGATYLLQ
LVFLTRDEMDGPIKLKAKLQEDQAHRIIISTIRIATKLLEDFVHSQNYICKVFGISKR
LLTKLEISFMASVNFDGLMITCEKLEKTLHILDDTRQALGNT"
gene <138698..>139409
/gene="SAR1"
/locus_tag="YPL218W"
/db_xref="GeneID:855883"
mRNA join(<138698..138725,138865..>139409)
/gene="SAR1"
/locus_tag="YPL218W"
/product="Arf family GTPase SAR1"
/transcript_id="NM_001184032.1"
/db_xref="GeneID:855883"
CDS join(138698..138725,138865..139409)
/gene="SAR1"
/locus_tag="YPL218W"
/experiment="EXISTENCE:direct assay:GO:0003400 regulation
of COPII vesicle coating [PMID:14627716|PMID:15665868]"
/experiment="EXISTENCE:direct assay:GO:0003924 GTPase
activity [PMID:8419365|PMID:9756629]"
/experiment="EXISTENCE:direct assay:GO:0016050 vesicle
organization [PMID:16122427]"
/experiment="EXISTENCE:direct assay:GO:0030127 COPII
vesicle coat [PMID:8004676]"
/experiment="EXISTENCE:direct assay:GO:0044233
mitochondria-associated endoplasmic reticulum membrane
contact site [PMID:27101143]"
/experiment="EXISTENCE:direct assay:GO:0070971 endoplasmic
reticulum exit site [PMID:22675024]"
/experiment="EXISTENCE:genetic interaction:GO:0000266
mitochondrial fission [PMID:27101143]"
/experiment="EXISTENCE:mutant phenotype:GO:0006998 nuclear
envelope organization [PMID:31406088]"
/experiment="EXISTENCE:mutant phenotype:GO:0007006
mitochondrial membrane organization [PMID:27101143]"
/experiment="EXISTENCE:mutant phenotype:GO:0044233
mitochondria-associated endoplasmic reticulum membrane
contact site [PMID:27101143]"
/experiment="EXISTENCE:mutant phenotype:GO:0061024
membrane organization [PMID:27101143]"
/note="ARF family GTPase; component of the COPII vesicle
coat; required for transport vesicle formation during ER
to Golgi protein transport; lowers membrane rigidity
aiding vesicle formation; localizes to ER-mitochondrial
contact sites where it enhances membrane curvature,
thereby reducing contact size via its N-terminal
amphipathic helix; regulates mitochondrial fission and
fusion dynamics"
/codon_start=1
/product="Arf family GTPase SAR1"
/protein_id="NP_015106.1"
/db_xref="GeneID:855883"
/db_xref="SGD:S000006139"
/translation="MAGWDIFGWFRDVLASLGLWNKHGKLLFLGLDNAGKTTLLHMLK
NDRLATLQPTWHPTSEELAIGNIKFTTFDLGGHIQARRLWKDYFPEVNGIVFLVDAAD
PERFDEARVELDALFNIAELKDVPFVILGNKIDAPNAVSEAELRSALGLLNTTGSQRI
EGQRPVEVFMCSVVMRNGYLEAFQWLSQYI"
gene complement(<139620..>143171)
/gene="BMS1"
/locus_tag="YPL217C"
/db_xref="GeneID:855884"
mRNA complement(<139620..>143171)
/gene="BMS1"
/locus_tag="YPL217C"
/product="GTPase BMS1"
/transcript_id="NM_001184031.1"
/db_xref="GeneID:855884"
CDS complement(139620..143171)
/gene="BMS1"
/locus_tag="YPL217C"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0003924 GTPase
activity [PMID:16307926]"
/experiment="EXISTENCE:direct assay:GO:0005525 GTP binding
[PMID:16307926]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10684247]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:10684247]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:10684247]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/experiment="EXISTENCE:direct assay:GO:0034511 U3 snoRNA
binding [PMID:16307926]"
/experiment="EXISTENCE:mutant phenotype:GO:0000472
endonucleolytic cleavage to generate mature 5'-end of
SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:11565749]"
/experiment="EXISTENCE:mutant phenotype:GO:0000480
endonucleolytic cleavage in 5'-ETS of tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:11565749]"
/experiment="EXISTENCE:mutant phenotype:GO:0003924 GTPase
activity [PMID:16307926]"
/experiment="EXISTENCE:mutant phenotype:GO:0005525 GTP
binding [PMID:16307926]"
/note="GTPase required for ribosomal subunit synthesis and
rRNA processing; required for synthesis of 40S ribosomal
subunits and for processing the 35S pre-rRNA at sites A0,
A1, and A2; interacts with Rcl1p, which stimulates its
GTPase and U3 snoRNA binding activities; has similarity to
Tsr1p"
/codon_start=1
/product="GTPase BMS1"
/protein_id="NP_015107.1"
/db_xref="GeneID:855884"
/db_xref="SGD:S000006138"
/translation="MEQSNKQHRKAKEKNTAKKKLHTQGHNAKAFAVAAPGKMARTMQ
RSSDVNERKLHVPMVDRTPEDDPPPFIVAVVGPPGTGKTTLIRSLVRRMTKSTLNDIQ
GPITVVSGKHRRLTFLECPADDLNAMIDIAKIADLVLLLIDGNFGFEMETMEFLNIAQ
HHGMPRVLGVATHLDLFKSQSTLRASKKRLKHRFWTEVYQGAKLFYLSGVINGRYPDR
EILNLSRFISVMKFRPLKWRNEHPYMLADRFTDLTHPELIETQGLQIDRKVAIYGYLH
GTPLPSAPGTRVHIAGVGDFSVAQIEKLPDPCPTPFYQQKLDDFEREKMKEEAKANGE
ITTASTTRRRKRLDDKDKLIYAPMSDVGGVLMDKDAVYIDIGKKNEEPSFVPGQERGE
GEKLMTGLQSVEQSIAEKFDGVGLQLFSNGTELHEVADHEGMDVESGEESIEDDEGKS
KGRTSLRKPRIYGKPVQEEDADIDNLPSDEEPYTNDDDVQDSEPRMVEIDFNNTGEQG
AEKLALETDSEFEESEDEFSWERTAANKLKKTESKKRTWNIGKLIYMDNISPEECIRR
WRGEDDDSKDESDIEEDVDDDFFRKKDGTVTKEGNKDHAVDLEKFVPYFDTFEKLAKK
WKSVDAIKERFLGAGILGNDNKTKSDSNEGGEELYGDFEDLEDGNPSEQAEDNSDKES
EDEDENEDTNGDDDNSFTNFDAEEKKDLTMEQEREMNAAKKEKLRAQFEIEEGENFKE
DDENNEYDTWYELQKAKISKQLEINNIEYQEMTPEQRQRIEGFKAGSYVRIVFEKVPM
EFVKNFNPKFPIVMGGLLPTEIKFGIVKARLRRHRWHKKILKTNDPLVLSLGWRRFQT
LPIYTTTDSRTRTRMLKYTPEHTYCNAAFYGPLCSPNTPFCGVQIVANSDTGNGFRIA
ATGIVEEIDVNIEIVKKLKLVGFPYKIFKNTAFIKDMFSSAMEVARFEGAQIKTVSGI
RGEIKRALSKPEGHYRAAFEDKILMSDIVILRSWYPVRVKKFYNPVTSLLLKEKTEWK
GLRLTGQIRAAMNLETPSNPDSAYHKIERVERHFNGLKVPKAVQKELPFKSQIHQMKP
QKKKTYMAKRAVVLGGDEKKARSFIQKVLTISKAKDSKRKEQKASQRKERLKKLAKME
EEKSQRDKEKKKEYFAQNGKRTTMGGDDESRPRKMRR"
gene <143821..>147129
/locus_tag="YPL216W"
/db_xref="GeneID:855885"
mRNA <143821..>147129
/locus_tag="YPL216W"
/product="uncharacterized protein"
/transcript_id="NM_001184030.1"
/db_xref="GeneID:855885"
CDS 143821..147129
/locus_tag="YPL216W"
/note="hypothetical protein; not an essential gene;
YPL216W has a paralog, ITC1, that arose from the whole
genome duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015108.1"
/db_xref="GeneID:855885"
/db_xref="SGD:S000006137"
/translation="MVLLNRRKIQPKEIGQSADSFSETPWVIKESSERINDYDSDLKK
LDFYKRDIFTCEISGKDGLSYFKALKSEEQHREKVRYLLPKELRKAIANFANFSPIRK
VGHLVESAFQRFSNRFFIGDTVCLKTIQKNALITYKEGEPNLVESPTIENNVTLFLVK
DVFQSNGMMESEKGEISAPKLSLYLITECLNRESKGAALIVGQNEIKRPESHFSKFII
ACFLNEILIKVSNKEHAPWRVKQEYIERYDVNPKCSPNMIDYLPDRMNSSSSELYTPL
TIPPESDVEPADWKETSETSETSETSLSKIKAIDDEISVSFDHIYDNVNSLAYNDLKG
TVDDKELPFTGPSIPFENISYLDSSLEYKNIDQKWFKECSQFPTERLLVVYQFLSFFG
RFIGLSHFNFDQFLTTIKCTSPEALVDEYVKINFLKTYNSKGSFTNEKPRNEIYNQVT
SSNVSQREKANVFNADESQRIPSNFTRNQKMRKFITDKSTEFVMYSIFKGKPLKNDDM
EFQSYEKVNILYIDIVCSLMCLMTDNEPDWNCNLMDNWTEEKRKEEGNKTEIDIAIEK
CLNYGDTSWVKLLHNKNFSNGNWLICLLGILQQNTHMIAYSDVAKCITKKILPLSMNF
VNLGDELWDNFRKRLSIKDKIDVLWVLVDFASNFSSYIKELVDKVPKLCNGIRLKLDS
AKKEYIKLKRQLKTLTKNRVKLHSNVSMNRYGSDECKGKVNALKVKIAYLMEDIAFLE
AKLIQSDIKRLEILGKDRNGNRYYWMDSNGSSSAISEKNEELYNCCFLWVQGPSEADI
NFCLDVDVESLKKWELLAKAKGTAYATKEVFSIFRSTDGSYYQIAQGENFMIINSNGI
LMRPTIPAFIDKKIISETPEKLLLSHHQWAFFNDIEDIHMLVDRLDDLRENEGQLKKA
LTSKMDRIEVSYKQQFKIKRRIECDETFKKNHKLLKNNEFTFPELKRIETTCTSNGQH
FSNMEKISKKLSRTKNDLVLEAILKDVAHLGECERALLKKQQNLIYPLNFHFEQLRTI
DLEFIVETKRKRQEDILTKLLNHQRYKHISHVSGYGISSQRVDKAAHLDVQGILEEIE
CQLISRRREDEERN"
gene <147416..>148423
/gene="CBP3"
/locus_tag="YPL215W"
/db_xref="GeneID:855886"
mRNA <147416..>148423
/gene="CBP3"
/locus_tag="YPL215W"
/product="Cbp3p"
/transcript_id="NM_001184029.1"
/db_xref="GeneID:855886"
CDS 147416..148423
/gene="CBP3"
/locus_tag="YPL215W"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:29475949]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239|PMID:11522252]"
/experiment="EXISTENCE:direct assay:GO:0005761
mitochondrial ribosome [PMID:21670217]"
/experiment="EXISTENCE:direct assay:GO:0031966
mitochondrial membrane [PMID:11522252]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:21670217]"
/experiment="EXISTENCE:mutant phenotype:GO:0034551
mitochondrial respiratory chain complex III assembly
[PMID:11522252|PMID:15759116]"
/experiment="EXISTENCE:mutant phenotype:GO:0070131
positive regulation of mitochondrial translation
[PMID:21670217]"
/experiment="EXISTENCE:physical interaction:GO:0043022
ribosome binding [PMID:21670217]"
/experiment="EXISTENCE:physical interaction:GO:0061671
Cbp3p-Cbp6 complex [PMID:21670217]"
/note="Mitochondrial protein required for assembly of
cytochrome bc1 complex; forms a complex with Cbp6p that
binds to mt ribosomes near the polypeptide tunnel exit and
promotes efficient translation of the COB mRNA;
Cbp3p-Cbp6p complex also interacts with newly synthesized
cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp
into the cytochrome bc1 complex; Cbp3p-Cbp6p complex is
sequestered if assembly of Complex III is blocked,
downregulating COB mRNA translation"
/codon_start=1
/product="Cbp3p"
/protein_id="NP_015109.1"
/db_xref="GeneID:855886"
/db_xref="SGD:S000006136"
/translation="MMSVNRFTSGRLPVFLRKSPFYYSRAYLHQTCVFKQNKETAQDS
PELLAKSSHLNSKPLDVSNKAPVKTAQNKIPLAHSKYESSKYELPKWKEALGELVIRA
FHLDMDRVRAGPVAGSYYYKICKEQGLQYEDEPLSETAKYFYEDLKLPRTFSQWFQIT
VLHEWILFVRMRAMPFKYGRNYQQKLVDRTFSDIELRLFEEMKVNSGRIADQYLKDFN
TQLRGAIFAYDEGFATDDGTLATAVWRNLFGGRKNIDMVHLESVVRYIYSQLYVLSRL
SDREFATGKFKFVPPGVKVEKLTPKQEEELKAKTIAKYEALDKDPKTLPSERSRLSYT
N"
gene complement(<148569..>150191)
/gene="THI6"
/locus_tag="YPL214C"
/db_xref="GeneID:855887"
mRNA complement(<148569..>150191)
/gene="THI6"
/locus_tag="YPL214C"
/product="bifunctional hydroxyethylthiazole
kinase/thiamine-phosphate diphosphorylase"
/transcript_id="NM_001184028.1"
/db_xref="GeneID:855887"
CDS complement(148569..150191)
/gene="THI6"
/locus_tag="YPL214C"
/EC_number="2.5.1.3"
/EC_number="2.7.1.50"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:16850348]"
/experiment="EXISTENCE:mutant phenotype:GO:0004417
hydroxyethylthiazole kinase activity [PMID:7982968]"
/experiment="EXISTENCE:mutant phenotype:GO:0004789
thiamine-phosphate diphosphorylase activity
[PMID:7982968]"
/experiment="EXISTENCE:mutant phenotype:GO:0009228
thiamine biosynthetic process [PMID:7982968]"
/note="Thiamine-phosphate diphosphorylase and
hydroxyethylthiazole kinase; required for thiamine
biosynthesis; GFP-fusion protein localizes to the
cytoplasm in a punctate pattern"
/codon_start=1
/product="bifunctional hydroxyethylthiazole
kinase/thiamine-phosphate diphosphorylase"
/protein_id="NP_015110.1"
/db_xref="GeneID:855887"
/db_xref="SGD:S000006135"
/translation="MVFTKEEVDYSLYLVTDSTMLPPGTTLCSQVEAGLKNGVTLVQI
REKDIETKNFVAEALEVQKICKKYNVPLIINDRIDVAMAIDADGVHVGQDDMPIPMVR
KLLGPSKILGWSVGKPSEVETLAKWGPDMVDYIGVGTLFPTSTKKNPKKSPMGPQGAI
AILDALEEFKATWCRTVGIGGLHPDNIQRVLCQCVASNGKRSLDGISLVSDIMAAPDA
CAATKRLRGLLDATRYQFVECELNNTFPTTTSIQNVISQVSNNRPLVQHITNKVHQNF
GANVTLALGSSPIMSEIESEVSELARIPNASLLLNTGSVAPIEMLKAAINAYNEVNRP
ITFDPVGYSATETRLCLNNTLLTYGQFACIKGNCSEILSLAKLNNHKMKGVDSSSGKT
NIDTLVRATQIVAFQYRTVAVCTGEFDCVADGTFGGEYKLSSGTEGITAEDLPCVIIE
DGPIPIMGDITASGCSLGSTIASFIGGLDSTGKLFDAVVGAVLLYKSAGKLASTRCQG
SGSFHVELIDALYQLFHENKPEKWSASLKKFK"
gene <150614..>151330
/gene="LEA1"
/locus_tag="YPL213W"
/db_xref="GeneID:855888"
mRNA <150614..>151330
/gene="LEA1"
/locus_tag="YPL213W"
/product="U2 snRNP complex subunit LEA1"
/transcript_id="NM_001184027.1"
/db_xref="GeneID:855888"
CDS 150614..151330
/gene="LEA1"
/locus_tag="YPL213W"
/experiment="EXISTENCE:direct assay:GO:0005686 U2 snRNP
[PMID:9799242]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0071004 U2-type
prespliceosome [PMID:16618970]"
/experiment="EXISTENCE:mutant phenotype:GO:0000398 mRNA
splicing, via spliceosome [PMID:9799242]"
/experiment="EXISTENCE:physical interaction:GO:0005686 U2
snRNP [PMID:9799242]"
/note="Component of U2 snRNP complex; disruption causes
reduced U2 snRNP levels; physically interacts with Msl1p;
putative homolog of human U2A' snRNP protein"
/codon_start=1
/product="U2 snRNP complex subunit LEA1"
/protein_id="NP_015111.1"
/db_xref="GeneID:855888"
/db_xref="SGD:S000006134"
/translation="MKFTPSIVIDAPQYYVDHFNGKYNVDKCVILRDLQLETDSESMP
SSLKHLTKPTHILDLTNNDLIMIPDLSRRDDIHTLLLGRNNIVEVDGRLLPMNVQNLT
LSNNSIRRFEDLQRLRRAPRTLKNLTLIGNQVCHLANYREHVLRLVPHLETLDFQNVT
AEERKSAMSFPRQADGDTLGPVNTAIRDNGSRDKTMEIMNLVVSKMTVERRNELKKQL
AEATSLEEIARLEKLLSGGV"
gene complement(<151515..>153149)
/gene="PUS1"
/locus_tag="YPL212C"
/db_xref="GeneID:855889"
mRNA complement(<151515..>153149)
/gene="PUS1"
/locus_tag="YPL212C"
/product="pseudouridine synthase PUS1"
/transcript_id="NM_001184026.1"
/db_xref="GeneID:855889"
CDS complement(151515..153149)
/gene="PUS1"
/locus_tag="YPL212C"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:8641292]"
/experiment="EXISTENCE:direct assay:GO:0009982
pseudouridine synthase activity [PMID:10356324]"
/experiment="EXISTENCE:direct assay:GO:0031119 tRNA
pseudouridine synthesis [PMID:10356324]"
/experiment="EXISTENCE:mutant phenotype:GO:0031120 snRNA
pseudouridine synthesis [PMID:10022901]"
/experiment="EXISTENCE:mutant phenotype:GO:1990481 mRNA
pseudouridine synthesis [PMID:25219674|PMID:25192136]"
/note="tRNA:pseudouridine synthase; introduces
pseudouridines at positions 26-28, 34-36, 65, and 67 of
tRNA; also acts on U2 snRNA; also pseudouridylates some
mRNAs, and pseudouridylation level varies with growth
phase; nuclear protein that appears to be involved in tRNA
export; PUS1 has a paralog, PUS2, that arose from the
whole genome duplication"
/codon_start=1
/product="pseudouridine synthase PUS1"
/protein_id="NP_015112.1"
/db_xref="GeneID:855889"
/db_xref="SGD:S000006133"
/translation="MSEENLRPAYDDQVNEDVYKRGAQSKLTKARKADFDDEKDKKKD
NDKHIDKRPKSGPRLDENGNPLPKEPRLPKRKVAVMVGYCGTGYHGMQYNPPNPTIES
ALFKAFVEAGAISKDNSNDLKKNGFMRAARTDKGVHAGGNLISLKMIIEDPDIKQKIN
EKLPEGIRVWDIERVNKAFDCRKMCSSRWYEYLLPTYSLIGPKPGSILYRDIEESKTE
LPGVLDEDLESKEFWEEFKKDANEKFSTEEIEAILAYVPPARDEFDINEELYQKVKKY
KQLENAHRRRYRISAAKLAKFRASTSQYLGAHNFHNFTLGKDFKEPSAIRFMKDIKVS
DPFVIGDAQTEWISIKIHGQSFMLHQIRKMVSMATLITRCGCPVERISQAYGQQKINI
PKAPALGLLLEAPVFEGYNKRLEQFGYKAIDFSKYQDEVDKFKMKHIYDKIYKEEVDE
NVFNAFFSYIDSFNKVTGAQGEETADKSGPAVQKSIFEFLTAKGIPGLTDAPESNKKI
KQRKRMEEEEAASKKAEISSTTQSNEPEVQPEAAAN"
gene <153495..>154040
/gene="NIP7"
/locus_tag="YPL211W"
/db_xref="GeneID:855890"
mRNA <153495..>154040
/gene="NIP7"
/locus_tag="YPL211W"
/product="ribosome biosynthesis protein NIP7"
/transcript_id="NM_001184025.1"
/db_xref="GeneID:855890"
CDS 153495..154040
/gene="NIP7"
/locus_tag="YPL211W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9271378|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:9271378]"
/experiment="EXISTENCE:direct assay:GO:0030687
preribosome, large subunit precursor
[PMID:11583614|PMID:17443350|PMID:23212245]"
/experiment="EXISTENCE:mutant phenotype:GO:0000463
maturation of LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:9271378]"
/experiment="EXISTENCE:mutant phenotype:GO:0042273
ribosomal large subunit biogenesis [PMID:9271378]"
/experiment="EXISTENCE:mutant phenotype:GO:1902626
assembly of large subunit precursor of preribosome
[PMID:22735702]"
/note="Nucleolar protein required for 60S ribosome subunit
biogenesis; constituent of 66S pre-ribosomal particles;
physically interacts with Nop8p and the exosome subunit
Rrp43p"
/codon_start=1
/product="ribosome biosynthesis protein NIP7"
/protein_id="NP_015113.1"
/db_xref="GeneID:855890"
/db_xref="SGD:S000006132"
/translation="MRQLTEEETKVVFEKLAGYIGRNISFLVDNKELPHVFRLQKDRV
YYVPDHVAKLATSVARPNLMSLGICLGKFTKTGKFRLHITSLTVLAKHAKYKIWIKPN
GEMPFLYGNHVLKAHVGKMSDDIPEHAGVIVFAMNDVPLGFGVSAKSTSESRNMQPTG
IVAFRQADIGEYLRDEDTLFT"
gene complement(<154290..>156212)
/gene="SRP72"
/locus_tag="YPL210C"
/db_xref="GeneID:855891"
mRNA complement(<154290..>156212)
/gene="SRP72"
/locus_tag="YPL210C"
/product="signal recognition particle subunit SRP72"
/transcript_id="NM_001184024.1"
/db_xref="GeneID:855891"
CDS complement(154290..156212)
/gene="SRP72"
/locus_tag="YPL210C"
/experiment="EXISTENCE:direct assay:GO:0005786 signal
recognition particle, endoplasmic reticulum targeting
[PMID:7925282]"
/experiment="EXISTENCE:direct assay:GO:0006614
SRP-dependent cotranslational protein targeting to
membrane [PMID:10921896]"
/note="Core component of the signal recognition particle
(SRP); the SRP is a ribonucleoprotein (RNP) complex that
functions in targeting nascent secretory proteins to the
endoplasmic reticulum (ER) membrane"
/codon_start=1
/product="signal recognition particle subunit SRP72"
/protein_id="NP_015114.2"
/db_xref="GeneID:855891"
/db_xref="SGD:S000006131"
/translation="MAKDNLTNLLSQLNIQLSQDEHSQVEQTCVKLLDSGCENPADVF
RRCLVAVIQQDKYQKALHYLKKFKHIDDKYGRKFALEKLYIFYKLNMPDEFNTLYTAI
ITDDLDTVLKKDIESLRGILHVRAQYCYKNGLYQEAFKIYQHLASHNEKDQDSQIELS
CNERVPLSVATELMNRSPLVTPMDESSYDLLFNESFIMASVGKYDKAIELLEKALQGA
TNEGYQNDINTIKLQLSFVLQMVGKTAQSKEILKGLLQELKADSPFSLICQNNLNAFV
DFSKYNTNFNLLLRELNVEKLNTFNLQTFTHEQWSNIQRNVLFLRLFNNVKIHSQESL
LSRTFDKYSKLVDNVTLESYKTQAKKLYHHTTKTILSGTDGSTIGILLLTIQLLIIEK
EWENAIRIGELFLNESWKSSFEKFNDSQAIVCYILFELYKIKGRNNSKSVLLKKLGSV
RVQLSGKIQENIPFWKHVGFELLSMGNAKESKALLREISNFSKGDADVLVDRVVSSDS
LDIAQGIDLVRDIDIDKLIQLGVKPLESSAKRSKNTAVSKVQKRKVLELKKKRKIKRL
EKFLQGRDTSKLPDPERWLPLRDRSTYRPKKKQQGAKQTQGGAMNKKSEQALDISKKG
KPTVNKKPKNKKKGRK"
gene complement(<156490..>157593)
/gene="IPL1"
/locus_tag="YPL209C"
/gene_synonym="PAC15"
/db_xref="GeneID:855892"
mRNA complement(<156490..>157593)
/gene="IPL1"
/locus_tag="YPL209C"
/gene_synonym="PAC15"
/product="aurora kinase"
/transcript_id="NM_001184023.1"
/db_xref="GeneID:855892"
CDS complement(156490..157593)
/gene="IPL1"
/locus_tag="YPL209C"
/gene_synonym="PAC15"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0000776 kinetochore
[PMID:11511347|PMID:12566427]"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity
[PMID:10072382|PMID:25387524|PMID:23142046|PMID:16319894|P
MID:11799062|PMID:16143104]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005819 spindle
[PMID:12566427]"
/experiment="EXISTENCE:direct assay:GO:0005828 kinetochore
microtubule [PMID:11724818]"
/experiment="EXISTENCE:direct assay:GO:0005874 microtubule
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005876 spindle
microtubule [PMID:11724818|PMID:11511347]"
/experiment="EXISTENCE:direct assay:GO:0032133 chromosome
passenger complex [PMID:19158380]"
/experiment="EXISTENCE:direct assay:GO:0051233 spindle
midzone [PMID:12566427]"
/experiment="EXISTENCE:genetic interaction:GO:0044774
mitotic DNA integrity checkpoint signaling
[PMID:16651657]"
/experiment="EXISTENCE:genetic interaction:GO:0044779
meiotic spindle checkpoint signaling [PMID:37018287]"
/experiment="EXISTENCE:mutant phenotype:GO:0007059
chromosome segregation [PMID:8293973]"
/experiment="EXISTENCE:mutant phenotype:GO:0008608
attachment of spindle microtubules to kinetochore
[PMID:10072382]"
/experiment="EXISTENCE:mutant phenotype:GO:0032465
regulation of cytokinesis [PMID:16615892]"
/experiment="EXISTENCE:mutant phenotype:GO:0045143
homologous chromosome segregation [PMID:17289568]"
/experiment="EXISTENCE:mutant phenotype:GO:0045144 meiotic
sister chromatid segregation
[PMID:17371833|PMID:17289568]"
/experiment="EXISTENCE:mutant phenotype:GO:0051228 mitotic
spindle disassembly [PMID:12566427]"
/experiment="EXISTENCE:mutant phenotype:GO:0090266
regulation of mitotic cell cycle spindle assembly
checkpoint [PMID:16327780]"
/experiment="EXISTENCE:mutant phenotype:GO:1901673
regulation of mitotic spindle assembly [PMID:24386320]"
/experiment="EXISTENCE:mutant phenotype:GO:1901925
negative regulation of protein import into nucleus during
spindle assembly checkpoint [PMID:23177738]"
/note="Aurora kinase of the chromosomal passenger complex;
mediates release of mono-oriented kinetochores from
microtubules in MI, and kinetochore release from SPB
clusters during meiotic exit; maintains meiotic spindle
checkpoint delay in a mek1 null; maintains condensed
chromosomes in anaphase; required for SPB cohesion and
preventing multipolar spindle formation; promotes
telomerase release at G2/M; Iocalizes to nuclear foci that
diffuse upon DNA replication stress; inhibits Pse1p upon
SAC arrest"
/codon_start=1
/product="aurora kinase"
/protein_id="NP_015115.1"
/db_xref="GeneID:855892"
/db_xref="SGD:S000006130"
/translation="MQRNSLVNIKLNANSPSKKTTTRPNTSRINKPWRISHSPQQRNP
NSKIPSPVREKLNRLPVNNKKFLDMESSKIPSPIRKATSSKMIHENKKLPKFKSLSLD
DFELGKKLGKGKFGKVYCVRHRSTGYICALKVMEKEEIIKYNLQKQFRREVEIQTSLN
HPNLTKSYGYFHDEKRVYLLMEYLVNGEMYKLLRLHGPFNDILASDYIYQIANALDYM
HKKNIIHRDIKPENILIGFNNVIKLTDFGWSIINPPENRRKTVCGTIDYLSPEMVESR
EYDHTIDAWALGVLAFELLTGAPPFEEEMKDTTYKRIAALDIKMPSNISQDAQDLILK
LLKYDPKDRMRLGDVKMHPWILRNKPFWENKRL"
gene <157842..>159593
/gene="RKM1"
/locus_tag="YPL208W"
/db_xref="GeneID:855893"
mRNA <157842..>159593
/gene="RKM1"
/locus_tag="YPL208W"
/product="protein-lysine N-methyltransferase"
/transcript_id="NM_001184022.1"
/db_xref="GeneID:855893"
CDS 157842..159593
/gene="RKM1"
/locus_tag="YPL208W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0016279
protein-lysine N-methyltransferase activity
[PMID:16096273]"
/experiment="EXISTENCE:direct assay:GO:0018027
peptidyl-lysine dimethylation [PMID:16096273]"
/experiment="EXISTENCE:mutant phenotype:GO:0016279
protein-lysine N-methyltransferase activity
[PMID:16096273]"
/experiment="EXISTENCE:mutant phenotype:GO:0018026
peptidyl-lysine monomethylation [PMID:22522802]"
/experiment="EXISTENCE:mutant phenotype:GO:0018027
peptidyl-lysine dimethylation [PMID:16096273]"
/note="SET-domain lysine-N-methyltransferase; catalyzes
the formation of dimethyllysine residues on the large
ribosomal subunit proteins L23 (Rpl23Ap and Rpl23Bp) and
monomethyllysine residues on L18 (Rps18Ap and Rps18Bp)"
/codon_start=1
/product="protein-lysine N-methyltransferase"
/protein_id="NP_015116.1"
/db_xref="GeneID:855893"
/db_xref="SGD:S000006129"
/translation="MSSDALKALLQWGASFGVIVPEELKFLYTDLKGIICVCEKDIDN
PSIKIPPEIVISRNLPMKFFGLSESTKNINGWLKLFFAKIKFDRDNDTIVDNVRVNDK
FKPYLDALPSRLNSPLVWNPSELKRLSSTNIGNSIHEKFEGIFKEWFELVSSSDMFDL
ERVADDVQTFHNLDELTYEALYEKILKITELQRPTIWYSFPAFLWSHLIFISRAFPEY
VLNRNCPDNSIVLLPIVDLLNHDYRSKVKWYPENGWFCYEKIGTASQSRELSNNYGGK
GNEELLSGYGFVLEDNIFDSVALKVKLPLDVVSTILETEPSLKLPLLSDYTTYAFENK
DCVQQEKKATRSATDYINGVTYFINIQNEQCLEPLLDLFTYLSKAEEEDLHDLRARLQ
GIQMLRNALQSKLNSITGPPATDDSYAIDPYRVYCADVYTKGQKQILKEALTRLKKLE
KTMLSENKHQLLTMSKILKNDPAFAETELPSLFSNEDGEEVIFESTYDLLILWILLKT
KKNSYPTKYEWVGQQYTNFKQTAYISDDAKAFHTAYFEKQDDVDLAEVDHAIQFVVDN
SFTRTSSTTEETILVRK"
gene <159909..>162341
/gene="TYW1"
/locus_tag="YPL207W"
/db_xref="GeneID:855894"
mRNA <159909..>162341
/gene="TYW1"
/locus_tag="YPL207W"
/product="putative tRNA 4-demethylwyosine synthase"
/transcript_id="NM_001184021.1"
/db_xref="GeneID:855894"
CDS 159909..162341
/gene="TYW1"
/locus_tag="YPL207W"
/EC_number="4.1.3.44"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0031591 wybutosine
biosynthetic process [PMID:34184886]"
/experiment="EXISTENCE:direct assay:GO:1904047
S-adenosyl-L-methionine binding [PMID:34184886]"
/experiment="EXISTENCE:mutant phenotype:GO:0031591
wybutosine biosynthetic process
[PMID:16642040|PMID:17150819|PMID:16162496]"
/note="Iron-sulfur protein required for synthesis of
wybutosine modified tRNA; wybutosine is a modified
guanosine found at 3'-position adjacent to anticodon of
phenylalanine tRNA which supports reading frame
maintenance by stabilizing codon-anticodon interactions;
induction by Yap5p in response to iron provides protection
from high iron toxicity; overexpression results in
increased cellular iron"
/codon_start=1
/product="putative tRNA 4-demethylwyosine synthase"
/protein_id="NP_015117.1"
/db_xref="GeneID:855894"
/db_xref="SGD:S000006128"
/translation="MDGFRVAGALVVGALTAAYLYFGGRFSIALVIIVGYGIYCNEAS
GGSQDSQEKLDLNKQQKKPCCSDKKIADGGKKTGGCCSDKKNGGGKGGGCCSSKGGKK
GGCCSSKGGKKGGCCSSKKNIGDNENTATEVEKAVNYPVTVDFTEVFRKPTKKRSSTP
KVFSKNSSSNSRVGKKLSVSKKIGPDGLIKSALTISNETLLSSQIYVLYSSLQGAASK
AAKSVYDKLKELDELTNEPKLLNLDDLSDFDDYFINVPVENALYVLVLPSYDIDCPLD
YFLQTLEENANDFRVDSFPLRKLVGYTVLGLGDSESWPEKFCYQAKRADHWISRLGGR
RIFPLGKVCMKTGGSAKIDEWTSLLAETLKDDEPIIYEYDENADSEEDEEEGNGSDEL
GDVEDIGGKGSNGKFSGADEIKQMVAKDSPTYKNLTKQGYKVIGSHSGVKICRWTKNE
LRGKGSCYKKSLFNIASSRCMELTPSLACSSKCVFCWRHGTNPVSKNWRWEVDEPEYI
LENALKGHYSMIKQMRGVPGVIAERFAKAFEVRHCALSLVGEPILYPHINKFIQLLHQ
KGITSFLVCNAQHPEALRNIVKVTQLYVSIDAPTKTELKKVDRPLYKDFWERMVECLE
ILKTVQNHQRTVFRLTLVKGFNMGDVSAYADLVQRGLPGFIEVKGATFSGSSDGNGNP
LTMQNIPFYEECVKFVKAFTTELQRRGLHYDLAAEHAHSNCLLIADTKFKINGEWHTH
IDFDKFFVLLNSGKDFTYMDYLEKTPEWALFGNGGFAPGNTRVYRKDKKKQNKENQET
TTRETPLPPIPA"
rep_origin 162341..162632
/note="ARS1608; Putative replication origin; identified in
multiple array studies, not yet confirmed by plasmid-based
assay"
/db_xref="SGD:S000130196"
gene complement(<162632..>163597)
/gene="PGC1"
/locus_tag="YPL206C"
/db_xref="GeneID:855895"
mRNA complement(<162632..>163597)
/gene="PGC1"
/locus_tag="YPL206C"
/product="phosphatidylglycerol phospholipase"
/transcript_id="NM_001184020.1"
/db_xref="GeneID:855895"
CDS complement(162632..163597)
/gene="PGC1"
/locus_tag="YPL206C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:18434318|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005811 lipid
droplet [PMID:12514182|PMID:24868093]"
/experiment="EXISTENCE:direct assay:GO:0034479
phosphatidylglycerol phospholipase C activity
[PMID:18434318]"
/experiment="EXISTENCE:mutant phenotype:GO:0034478
phosphatidylglycerol catabolic process [PMID:18434318]"
/experiment="EXISTENCE:mutant phenotype:GO:0034479
phosphatidylglycerol phospholipase C activity
[PMID:18434318]"
/experiment="EXISTENCE:mutant phenotype:GO:0046475
glycerophospholipid catabolic process [PMID:16172116]"
/note="Phosphatidylglycerol phospholipase C; regulates
phosphatidylglycerol (PG) accumulation via a phospholipase
C-type degradation mechanism; PG levels affect
mitochondrial function; contains glycerophosphodiester
phosphodiesterase motifs"
/codon_start=1
/product="phosphatidylglycerol phospholipase"
/protein_id="NP_015118.1"
/db_xref="GeneID:855895"
/db_xref="SGD:S000006127"
/translation="MVEIVGHRAFKARYPENTLLAFEKAYAAGADVIETDLQMTSDGM
VVVNHDSDTGRMWDKNLVIGESTWEEVKRLRCKEDGSLAMMTLKEILTWAVCHPGAKL
MLDIKFTNEKIIMIKTFVIMLEVKNDLKFWQERITWGLWLLDWYDFGIETGVLKDFKV
IVISLSLDIASQFVKRSLTLNDPHYKLFGISVHFVSSWTSQFRLRLLPVLMKNDIKVY
LWTVNKPIDFKYLCELPIHGAITDDPIKARKLCDGHTVAKKPTAEKKFVAPSLASVDG
LRFHAFIKVYNILCTLLYSKWVHIKLCGWSIAYVIFLFLRTIHFL"
gene <164276..>165760
/gene="HRR25"
/locus_tag="YPL204W"
/gene_synonym="KTI14"
/db_xref="GeneID:855897"
mRNA <164276..>165760
/gene="HRR25"
/locus_tag="YPL204W"
/gene_synonym="KTI14"
/product="serine/threonine protein kinase HRR25"
/transcript_id="NM_001184018.1"
/db_xref="GeneID:855897"
CDS 164276..165760
/gene="HRR25"
/locus_tag="YPL204W"
/gene_synonym="KTI14"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0000775 chromosome,
centromeric region [PMID:16990132]"
/experiment="EXISTENCE:direct assay:GO:0000932 P-body
[PMID:29915153]"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0004674 protein
serine/threonine kinase activity
[PMID:25569479|PMID:1495994|PMID:21532587]"
/experiment="EXISTENCE:direct assay:GO:0004713 protein
tyrosine kinase activity [PMID:7803855]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:8034689]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:21532587]"
/experiment="EXISTENCE:direct assay:GO:0005816 spindle
pole body [PMID:17461799]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:8034689]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:14597664|PMID:17461799]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:17461799|PMID:26928762|PMID:14597664]"
/experiment="EXISTENCE:direct assay:GO:0033551 monopolin
complex [PMID:16990132]"
/experiment="EXISTENCE:direct assay:GO:0042274 ribosomal
small subunit biogenesis [PMID:25778921]"
/experiment="EXISTENCE:direct assay:GO:0060628 regulation
of ER to Golgi vesicle-mediated transport [PMID:21532587]"
/experiment="EXISTENCE:mutant phenotype:GO:0000425
pexophagy [PMID:25287303]"
/experiment="EXISTENCE:mutant phenotype:GO:0002098 tRNA
wobble uridine modification [PMID:18755837]"
/experiment="EXISTENCE:mutant phenotype:GO:0004674 protein
serine/threonine kinase activity [PMID:16990132]"
/experiment="EXISTENCE:mutant phenotype:GO:0006281 DNA
repair [PMID:1887218]"
/experiment="EXISTENCE:mutant phenotype:GO:0032880
regulation of protein localization [PMID:14597664]"
/experiment="EXISTENCE:mutant phenotype:GO:0033551
monopolin complex [PMID:16990132]"
/experiment="EXISTENCE:mutant phenotype:GO:0042273
ribosomal large subunit biogenesis [PMID:18256024]"
/experiment="EXISTENCE:mutant phenotype:GO:0042274
ribosomal small subunit biogenesis
[PMID:16738661|PMID:25778921]"
/experiment="EXISTENCE:mutant phenotype:GO:0051455 spindle
attachment to meiosis I kinetochore [PMID:16990132]"
/experiment="EXISTENCE:mutant phenotype:GO:0060628
regulation of ER to Golgi vesicle-mediated transport
[PMID:21532587]"
/experiment="EXISTENCE:mutant phenotype:GO:0106214
regulation of vesicle fusion with Golgi apparatus
[PMID:26195667]"
/experiment="EXISTENCE:mutant phenotype:GO:2000370
positive regulation of clathrin-dependent endocytosis
[PMID:25287303]"
/experiment="EXISTENCE:mutant phenotype:GO:2000785
regulation of autophagosome assembly [PMID:26195667]"
/experiment="EXISTENCE:mutant phenotype:GO:2001159
regulation of protein localization by the Cvt pathway
[PMID:25287303|PMID:24968893]"
/experiment="EXISTENCE:physical interaction:GO:0033551
monopolin complex [PMID:16990132]"
/note="Casein kinase and monopolin subunit; regulates
diverse events including vesicular traffic, DNA repair,
the CVT pathway, ribosomal subunit biogenesis, cohesin
cleavage, monopolar attachment of sister kinetochores at
MI and exit from MII; autophosphorylation regulates
elongator-dependent tRNA modifications; binds the RNAPII
CTD; phosphorylates COPII coat subunits; antagonizes
phosphatase Sit4p; inhibits Crz1p and calcineurin
signaling; phosphorylates Dsn1p; functional ortholog of
human CK1epsilon"
/codon_start=1
/product="serine/threonine protein kinase HRR25"
/protein_id="NP_015120.1"
/db_xref="GeneID:855897"
/db_xref="SGD:S000006125"
/translation="MDLRVGRKFRIGRKIGSGSFGDIYHGTNLISGEEVAIKLESIRS
RHPQLDYESRVYRYLSGGVGIPFIRWFGREGEYNAMVIDLLGPSLEDLFNYCHRRFSF
KTVIMLALQMFCRIQYIHGRSFIHRDIKPDNFLMGVGRRGSTVHVIDFGLSKKYRDFN
THRHIPYRENKSLTGTARYASVNTHLGIEQSRRDDLESLGYVLIYFCKGSLPWQGLKA
TTKKQKYDRIMEKKLNVSVETLCSGLPLEFQEYMAYCKNLKFDEKPDYLFLARLFKDL
SIKLEYHNDHLFDWTMLRYTKAMVEKQRDLLIEKGDLNANSNAASASNSTDNKSETFN
KIKLLAMKKFPTHFHYYKNEDKHNPSPEEIKQQTILNNNAASSLPEELLNALDKGMEN
LRQQQPQQQVQSSQPQPQPQQLQQQPNGQRPNYYPEPLLQQQQRDSQEQQQQVPMATT
RATQYPPQINSNNFNTNQASVPPQMRSNPQQPPQDKPAGQSIWL"
gene <166256..>167398
/gene="TPK2"
/locus_tag="YPL203W"
/gene_synonym="PKA2; PKA3; YKR1"
/db_xref="GeneID:855898"
mRNA <166256..>167398
/gene="TPK2"
/locus_tag="YPL203W"
/gene_synonym="PKA2; PKA3; YKR1"
/product="cAMP-dependent protein kinase catalytic subunit
TPK2"
/transcript_id="NM_001184017.1"
/db_xref="GeneID:855898"
CDS 166256..167398
/gene="TPK2"
/locus_tag="YPL203W"
/gene_synonym="PKA2; PKA3; YKR1"
/EC_number="2.7.11.11"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:26403272]"
/experiment="EXISTENCE:direct assay:GO:0000932 P-body
[PMID:22899713]"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0004691
cAMP-dependent protein kinase activity [PMID:12024012]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476|PMID:12024012|PMID:18417610]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005952
cAMP-dependent protein kinase complex [PMID:12024012]"
/experiment="EXISTENCE:genetic interaction:GO:0004691
cAMP-dependent protein kinase activity [PMID:3036373]"
/experiment="EXISTENCE:genetic interaction:GO:0005952
cAMP-dependent protein kinase complex [PMID:3036373]"
/experiment="EXISTENCE:genetic interaction:GO:0007265 Ras
protein signal transduction [PMID:3036373]"
/experiment="EXISTENCE:genetic interaction:GO:0010607
negative regulation of cytoplasmic mRNA processing body
assembly [PMID:21925385]"
/experiment="EXISTENCE:mutant phenotype:GO:0001403
invasive growth in response to glucose limitation
[PMID:12150916]"
/experiment="EXISTENCE:mutant phenotype:GO:0070314 G1 to
G0 transition [PMID:22899713]"
/experiment="EXISTENCE:mutant phenotype:GO:0071333
cellular response to glucose stimulus [PMID:36167807]"
/experiment="EXISTENCE:mutant phenotype:GO:2000766
negative regulation of cytoplasmic translation
[PMID:22899713]"
/experiment="EXISTENCE:physical interaction:GO:0005952
cAMP-dependent protein kinase complex [PMID:12024012]"
/note="cAMP-dependent protein kinase catalytic subunit;
promotes nutrient responsive growth via the Ras-cAMP
signaling pathway; phosphorylates and inhibits histone
demethylase Jhd2p in response to glucose, promoting H3K4
trimethylation and maintaining H3K14 acetylation by
preventing chromatin binding of Rpd3p; required for
Sfl1p-mediated pseudohyphal growth; partially redundant
with Tpk1p and Tpk3p; localizes to P-bodies during
stationary phase; relocalizes to the cytosol in response
to hypoxia"
/codon_start=1
/product="cAMP-dependent protein kinase catalytic subunit
TPK2"
/protein_id="NP_015121.1"
/db_xref="GeneID:855898"
/db_xref="SGD:S000006124"
/translation="MEFVAERAQPVGQTIQQQNVNTYGQGVLQPHHDLQQRQQQQQQR
QHQQLLTSQLPQKSLVSKGKYTLHDFQIMRTLGTGSFGRVHLVRSVHNGRYYAIKVLK
KQQVVKMKQVEHTNDERRMLKLVEHPFLIRMWGTFQDARNIFMVMDYIEGGELFSLLR
KSQRFPNPVAKFYAAEVILALEYLHAHNIIYRDLKPENILLDRNGHIKITDFGFAKEV
QTVTWTLCGTPDYIAPEVITTKPYNKSVDWWSLGVLIYEMLAGYTPFYDTTPMKTYEK
ILQGKVVYPPYFHPDVVDLLSKLITADLTRRIGNLQSGSRDIKAHPWFSEVVWERLLA
KDIETPYEPPITSGIGDTSLFDQYPEEQLDYGIQGDDPYAEYFQDF"
gene complement(<168088..>169338)
/gene="AFT2"
/locus_tag="YPL202C"
/db_xref="GeneID:855899"
mRNA complement(<168088..>169338)
/gene="AFT2"
/locus_tag="YPL202C"
/product="Aft2p"
/transcript_id="NM_001184016.1"
/db_xref="GeneID:855899"
CDS complement(168088..169338)
/gene="AFT2"
/locus_tag="YPL202C"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:16024809]"
/experiment="EXISTENCE:direct assay:GO:0000981 DNA-binding
transcription factor activity, RNA polymerase II-specific
[PMID:27402848]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0045944 positive
regulation of transcription by RNA polymerase II
[PMID:11448968]"
/experiment="EXISTENCE:genetic interaction:GO:0006879
intracellular iron ion homeostasis [PMID:16024809]"
/experiment="EXISTENCE:genetic interaction:GO:0010106
cellular response to iron ion starvation [PMID:16024809]"
/experiment="EXISTENCE:genetic interaction:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:16024809]"
/experiment="EXISTENCE:mutant phenotype:GO:0032048
cardiolipin metabolic process [PMID:27402848]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:27402848]"
/experiment="EXISTENCE:mutant phenotype:GO:0051536
iron-sulfur cluster binding [PMID:31493153]"
/experiment="EXISTENCE:mutant phenotype:GO:2000185
regulation of phosphate transmembrane transport
[PMID:27618952]"
/note="Iron-regulated transcriptional activator; activates
genes involved in intracellular iron use and required for
iron homeostasis and resistance to oxidative stress; AFT2
has a paralog, AFT1, that arose from the whole genome
duplication"
/codon_start=1
/product="Aft2p"
/protein_id="NP_015122.1"
/db_xref="GeneID:855899"
/db_xref="SGD:S000006123"
/translation="MKAKSMKSIISVPISVSKTGKMKLTASPDNLASMMSKDQNKLIH
LDPVPSFEDRHEIKPWLQKIFYPQGIDIVIERSDSSKVTFKCRSVRSKVGLNPKSKGS
SSRSHACPFRIRAAYSVRLQKWNVVVMNNIHSHELRFDLITKTDDYKKFKENLRQKND
EKAIKTFDELEYKASLNLPLVTPIISCDCGLTKEIEAFNNIFLPLSNPPLTSKKNLLK
TNKNSVSKIKSRQMDNSKPRPRLKTKLDADLHDTGFLDNFKTRNSCVKIEKEDSLTNL
NEIDFTNMFCNDNFIQNYNQGLMELLTEPTPGPSSSSCILPSTPTRPLSQSKMDIALS
ESTTSSPNFMETDAPYGDEIIKVSKDTKSNAPTADTDIATNLGKERNENFGMLNYNYE
ALLHFNDEHFNELNSIDPALISKY"
gene complement(<169770..>171155)
/gene="YIG1"
/locus_tag="YPL201C"
/db_xref="GeneID:855900"
mRNA complement(<169770..>171155)
/gene="YIG1"
/locus_tag="YPL201C"
/product="Yig1p"
/transcript_id="NM_001184015.1"
/db_xref="GeneID:855900"
CDS complement(169770..171155)
/gene="YIG1"
/locus_tag="YPL201C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:16358322]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:16358322]"
/experiment="EXISTENCE:mutant phenotype:GO:0006114
glycerol biosynthetic process [PMID:16358322]"
/note="Protein that interacts with glycerol 3-phosphatase;
plays a role in anaerobic glycerol production; localizes
to the nucleus and cytosol"
/codon_start=1
/product="Yig1p"
/protein_id="NP_015123.1"
/db_xref="GeneID:855900"
/db_xref="SGD:S000006122"
/translation="MGIPMQIYQDGKGVQFYHTRYQNVFDERASKYGNYTVNNDYPQL
PDTIKEHIDQLTFSNVGEDGGDVGNYSEEDDDGDEEKELEDVFRSNRGLEFVRINNYF
TTHDLQSFKSFRNFNSKYWIFYSNQAEDKKLLLYDFNGQHLIFIKQQFYGQLNLLLSD
AIICMDCNFGYNSNTIQILVGFQNGKLLKLNCDLNGNVNNHLLLKDPSTSSHQSHLSI
LNVWAGLLPHFVVSFSLKDGLLITSLDHQQSNGSFQSFHTNIDLPVDLRTTTNVKSVL
NFPQFTLYKGNDMIFHCKNLLGSDASTLNKEINFMLKIDEDVQKIDYLLKTNHILLET
NMRYLSIPTRDPIENSNSSPPVSDSEVYPIFYKTQELHVHASGTGRQIANNGKYIFIT
EQHLYGTALSVYKYSISFKRWLFVGYSDIRAKYGIRSVKDLFVGNCPSVNSPVLTILT
DDNNIQTILLK"
gene <171484..>171954
/gene="CSM4"
/locus_tag="YPL200W"
/db_xref="GeneID:855901"
mRNA <171484..>171954
/gene="CSM4"
/locus_tag="YPL200W"
/product="Csm4p"
/transcript_id="NM_001184014.1"
/db_xref="GeneID:855901"
CDS 171484..171954
/gene="CSM4"
/locus_tag="YPL200W"
/experiment="EXISTENCE:direct assay:GO:0000781 chromosome,
telomeric region [PMID:18585352]"
/experiment="EXISTENCE:direct assay:GO:0005640 nuclear
outer membrane [PMID:32059771]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:12514182]"
/experiment="EXISTENCE:genetic interaction:GO:0045141
meiotic telomere clustering [PMID:18585352]"
/experiment="EXISTENCE:mutant phenotype:GO:0007129
homologous chromosome pairing at meiosis [PMID:21339478]"
/experiment="EXISTENCE:mutant phenotype:GO:0010520
regulation of reciprocal meiotic recombination
[PMID:18818742|PMID:18818741]"
/experiment="EXISTENCE:mutant phenotype:GO:0034993 meiotic
nuclear membrane microtubule tethering complex
[PMID:32059771]"
/experiment="EXISTENCE:mutant phenotype:GO:0045132 meiotic
chromosome segregation [PMID:11470404]"
/experiment="EXISTENCE:mutant phenotype:GO:0045141 meiotic
telomere clustering [PMID:18585352|PMID:18818742]"
/experiment="EXISTENCE:mutant phenotype:GO:0140444
cytoskeleton-nuclear membrane anchor activity
[PMID:32059771]"
/note="Protein required for accurate chromosome
segregation during meiosis; involved in meiotic telomere
clustering (bouquet formation) and telomere-led rapid
prophase movements; functions with meiosis-specific
telomere-binding protein Ndj1p; CSM4 has a paralog, MPS2,
that arose from the whole genome duplication"
/codon_start=1
/product="Csm4p"
/protein_id="NP_015124.1"
/db_xref="GeneID:855901"
/db_xref="SGD:S000006121"
/translation="MMDGSITRKVTSTLSNQLATWKWKLQLSLLERKLATINNDYFLL
QWELLFITNEVMKWKEMIAFLESQLFCTTQNFVAQETHDRETFQSLVDDYNKQLSENN
LIISVLKSRPQLSSFPIYLSDEVCSHLKFVIAELNSLIIVFFISLVFLWVSIEV"
gene complement(<172033..>172755)
/locus_tag="YPL199C"
/db_xref="GeneID:855902"
mRNA complement(<172033..>172755)
/locus_tag="YPL199C"
/product="Smr domain-containing protein"
/transcript_id="NM_001184013.1"
/db_xref="GeneID:855902"
CDS complement(172033..172755)
/locus_tag="YPL199C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0070481
nuclear-transcribed mRNA catabolic process, non-stop decay
[PMID:32234470]"
/note="Protein involved in nonstop mRNA decay; predicted
to be palmitoylated"
/codon_start=1
/product="Smr domain-containing protein"
/protein_id="NP_015125.1"
/db_xref="GeneID:855902"
/db_xref="SGD:S000006120"
/translation="MKGTGGVVVGTQNPVRDYNHSTDEEYQRLRRLADEAYKKRDQLS
HESQTAYQQGDKKLAHELSEKSKAQLKTAEDFNMQAAEYVFVENNADSSSNEIDLHGL
YVKEALFILQKRIKFAIDHNEPQLNVIVGKGLHSQNGIAKLKPSIEEFCAKHGIRNHL
EKGNSGVLVLELQGVQMQMDGPAVNAPTNQYNAQPHPQYNNNGGQPQGQAQNYNNSGN
DNKDSTLTSIFKIFCNCIQSLA"
gene <173152..>174702
/gene="RPL7B"
/locus_tag="YPL198W"
/db_xref="GeneID:855903"
mRNA join(<173152..173162,173572..173665,174073..>174702)
/gene="RPL7B"
/locus_tag="YPL198W"
/product="60S ribosomal protein uL30 RPL7B"
/transcript_id="NM_001184012.1"
/db_xref="GeneID:855903"
CDS join(173152..173162,173572..173665,174073..174702)
/gene="RPL7B"
/locus_tag="YPL198W"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:23874617]"
/experiment="EXISTENCE:curator inference:GO:0003735
structural constituent of ribosome [PMID:23874617]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:19466602]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:19466602]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:23874617]"
/experiment="EXISTENCE:direct assay:GO:0042273 ribosomal
large subunit biogenesis [PMID:23945946]"
/experiment="EXISTENCE:mutant phenotype:GO:0000470
maturation of LSU-rRNA [PMID:22893726]"
/note="Ribosomal 60S subunit protein L7B; required for
processing of 27SA3 pre-rRNA to 27SB pre-rRNA during
assembly of large ribosomal subunit; depletion leads to a
turnover of pre-rRNA; contains a conserved C-terminal
Nucleic acid Binding Domain (NDB2); binds to Domain II of
25S and 5.8S rRNAs; homologous to mammalian ribosomal
protein L7 and bacterial L30; RPL7B has a paralog, RPL7A,
that arose from the whole genome duplication"
/codon_start=1
/product="60S ribosomal protein uL30 RPL7B"
/protein_id="NP_015126.1"
/db_xref="GeneID:855903"
/db_xref="SGD:S000006119"
/translation="MSTEKILTPESQLKKTKAQQKTAEQIAAERAARKAANKEKRAII
LERNAAYQKEYETAERNIIQAKRDAKAAGSYYVEAQHKLVFVVRIKGINKIPPKPRKV
LQLLRLTRINSGTFVKVTKATLELLKLIEPYVAYGYPSYSTIRQLVYKRGFGKINKQR
VPLSDNAIIEANLGKYGILSIDDLIHEIITVGPHFKQANNFLWPFKLSNPSGGWGVPR
KFKHFIQGGSFGNREEFINKLVKAMN"
gene 173827..173904
/gene="SNR59"
/locus_tag="YNCP0003W"
/db_xref="GeneID:9164987"
ncRNA 173827..173904
/ncRNA_class="snoRNA"
/gene="SNR59"
/locus_tag="YNCP0003W"
/product="SNR59"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:10024243]"
/experiment="EXISTENCE:curator inference:GO:0031428 box
C/D methylation guide snoRNP complex [PMID:10024243]"
/note="C/D box small nucleolar RNA (snoRNA); guides
2'-O-methylation of large subunit (LSU) rRNA at position
A807"
/transcript_id="NR_132266.1"
/db_xref="GeneID:9164987"
/db_xref="SGD:S000006450"
gene <175043..>175864
/gene="OXR1"
/locus_tag="YPL196W"
/db_xref="GeneID:855905"
mRNA <175043..>175864
/gene="OXR1"
/locus_tag="YPL196W"
/product="Oxr1p"
/transcript_id="NM_001184010.1"
/db_xref="GeneID:855905"
CDS 175043..175864
/gene="OXR1"
/locus_tag="YPL196W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:38565737]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:15060142]"
/experiment="EXISTENCE:direct assay:GO:0032984
protein-containing complex disassembly [PMID:34918374]"
/experiment="EXISTENCE:mutant phenotype:GO:0032984
protein-containing complex disassembly [PMID:38565737]"
/experiment="EXISTENCE:mutant phenotype:GO:0045053 protein
retention in Golgi apparatus [PMID:38589611]"
/note="Protein that regulates the vacuolar V-ATPase;
inhibits V1-ATPase activity and triggers ATP-dependent
disassembly of the V-ATPase holoenzyme, counteracting the
function of the RAVE complex; localizes to the cytoplasm;
required for retention of the vacuolar-ATPase V0 domain
subunit Stv1p in pre-vacuolar compartments; involved in
resistance to oxidative damage; member of a conserved
family of TLDc domain proteins found in eukaryotes;
contains an N-terminal EF-hand-like domain"
/codon_start=1
/product="Oxr1p"
/protein_id="NP_015128.1"
/db_xref="GeneID:855905"
/db_xref="SGD:S000006117"
/translation="MFGVKDAIFKIKRSIAGTDSSDSTAYTTASESSPQLKDSHNPFR
NKTTSERTIVEEGSLPPVRLNGYLPSTKNKLLTPEMCDEIRTLMPTRIQLYTEWNLLY
SLEQHGSSLHSLYSNVAPDSKEFRRVGYVLVIKDRKNGIFGAYSNEAFHPNEHRQYTG
NGECFLWKLDKVPDVNISEKEESEQEGKEGKEEGDKEERWRFSGYPYTGVNEFAIYCT
SEFLSMGAGDGHYGLLCDDGLLHGVSNPCQTYGNEVLSKEGKKFSIVALEVWRVG"
gene <176223..>179021
/gene="APL5"
/locus_tag="YPL195W"
/gene_synonym="YKS4"
/db_xref="GeneID:855906"
mRNA <176223..>179021
/gene="APL5"
/locus_tag="YPL195W"
/gene_synonym="YKS4"
/product="Apl5p"
/transcript_id="NM_001184009.1"
/db_xref="GeneID:855906"
CDS 176223..179021
/gene="APL5"
/locus_tag="YPL195W"
/gene_synonym="YKS4"
/experiment="EXISTENCE:mutant phenotype:GO:0006623 protein
targeting to vacuole [PMID:17895371]"
/experiment="EXISTENCE:mutant phenotype:GO:0006896 Golgi
to vacuole transport [PMID:9335339]"
/experiment="EXISTENCE:mutant phenotype:GO:0030123 AP-3
adaptor complex [PMID:9250663]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:10559961]"
/note="Delta adaptin-like subunit of the clathrin
associated protein complex; functions in transport of
alkaline phosphatase to the vacuole via the alternate
pathway; suppressor of loss of casein kinase 1 function;
the clathrin associated protein complex is also known as
AP-3"
/codon_start=1
/product="Apl5p"
/protein_id="NP_015129.1"
/db_xref="GeneID:855906"
/db_xref="SGD:S000006116"
/translation="MTSLYAPGAEDIRQRLRPFGFFFEKSLKDLIKGIRSHNETPEKL
DQFFKQVLSECREEVNSPDLNSKTNAVLKLTYLEMYGFDMAWCNFHILEVMSSNKLQQ
KRVGYLAASQSFYKDSDILMLATNLLKKDLKYDGNNDVVKVGIALSGLSTIITPSLAR
DIADDLFTMLNSTRPYIRKKAITALFKVFLQYPEALRDNFDKFVSKLDDDDISVVSAA
VSVICELSKKNPQPFIQLSPLLYEILVTIDNNWIIIRLLKLFTNLSQVEPKLRAKLLP
KILELMESTVATSVIYESVNCIVKGNMLEEDDFETAMACLERLHTFCDSQDPNLRYIS
CILFYKIGKINTDFISRFDQLIIRLLSDVDVSIRSKAIELVEGIVDEDNLKAIVQTLM
KQFVDEDVVILQTGSIVYEKSKRIPIIIPENYKIKMVNVIISICSADNYSSVNDFEWY
NAVIMDLAMLCQDISDKSLGSKIGEQFRNLMIKVPSMREVTIANIIKLISNDNINKQL
PTVLRECIWCLGEFSTLVENGNDLIKIMTENISYYSHSVQEVLILALVKVFSNWCNNF
QEDKRFEIKMVLKELIEFFENLSYSSTFEVQERSVEVLEFLRLSLEALEEDTEGLPML
LSEVLPSFFNAYELAPIARGTQLKLAVDENLDLETPFLTKEAADELLDEQKSDAISDL
MSDISMDEQVELKFVDDSDTSYEEKEKLDDFENPFEIEREKERMSNPYYLGEEDEERT
KNSKDLLDLNEEESSDKKPETIRLNRTDNSLNSLSLSTTEISRKKKKGKKKNRVQVLS
DEPVIEAAPKRKDAFQKPHDNHSTQNPLKKDKINLRMHSQLENFDFSNFGQSSNAGRG
SQEEGNLRKEDELELSRLEANLIVKDEKDNLSDTEEVIVIKKKKKGKKSKSKNKLKTK
AKNSPEPNEFLRDQSTDI"
gene <179277..>181115
/gene="DDC1"
/locus_tag="YPL194W"
/db_xref="GeneID:855907"
mRNA <179277..>181115
/gene="DDC1"
/locus_tag="YPL194W"
/product="Ddc1p"
/transcript_id="NM_001184008.1"
/db_xref="GeneID:855907"
CDS 179277..181115
/gene="DDC1"
/locus_tag="YPL194W"
/experiment="EXISTENCE:direct assay:GO:0000725
recombinational repair [PMID:16840526]"
/experiment="EXISTENCE:direct assay:GO:0000794 condensed
nuclear chromosome [PMID:11825877]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0030295 protein
kinase activator activity [PMID:31340146]"
/experiment="EXISTENCE:direct assay:GO:0030896 checkpoint
clamp complex [PMID:12604797|PMID:9891048|PMID:9670034]"
/experiment="EXISTENCE:genetic interaction:GO:0000725
recombinational repair [PMID:16840526]"
/experiment="EXISTENCE:mutant phenotype:GO:0007095 mitotic
G2 DNA damage checkpoint signaling [PMID:9311982]"
/experiment="EXISTENCE:mutant phenotype:GO:0031571 mitotic
G1 DNA damage checkpoint signaling [PMID:9311982]"
/experiment="EXISTENCE:mutant phenotype:GO:0031573 mitotic
intra-S DNA damage checkpoint signaling [PMID:9311982]"
/experiment="EXISTENCE:mutant phenotype:GO:0051321 meiotic
cell cycle [PMID:11825877]"
/experiment="EXISTENCE:mutant phenotype:GO:0051598 meiotic
recombination checkpoint signaling [PMID:11825877]"
/note="DNA damage checkpoint protein; part of a PCNA-like
complex required for DNA damage response, required for
pachytene checkpoint to inhibit cell cycle in response to
unrepaired recombination intermediates; potential Cdc28p
substrate; forms nuclear foci upon DNA replication stress"
/codon_start=1
/product="Ddc1p"
/protein_id="NP_015130.1"
/db_xref="GeneID:855907"
/db_xref="SGD:S000006115"
/translation="MSFKATITESGKQNIWFRAIYVLSTIQDDIKITVTTNELIAWSM
NETDTTLCQVRFQKSFFEEYEFKPHEIVFGENGVQVIEDTYGNSHKLYSFRVNGRHLT
TISRKPDGDGIKSFTIAVNNTSTCPESLANRLIVVIEMDSLIVKEYCPQFQPIKYDPI
IINLKYKRRFLDVFGTAASDRNPQEPLDPKLLDVFTNTERELTSALFNEEVESDIRKR
NQLTAADEINYICCNSTLLKNFLDNCNVNVTDEVKLEINVHRLSITAFTKAVYGKNND
LLRNALSMSNTISTLDLEHYCLFTTIEDEKQDKRSHSKRREHMKSIIFKLKDFKNFIT
IGPSWKTTQDGNDNISLWFCHPGDPILMQMQKPGVKLELVEVTDSNINDDILEGKFIK
TAISGSKEEAGLKDNKESCESPLKSKTALKRENLPHSVAGTRNSPLKVSYLTPDNGST
VAKTYRNNTARKLFVEEQSQSTNYEQDKRFRQASSVHMNMNREQSFDIGTTHEVACPR
NESNSLKRSIADICNETEDPTQQSTFAKRADTTVTWGKALPAADDEVSCSNIDRKGML
KKEKLKHMQGLLNSQNDTSNHKKQDNKEMEDGLGLTQVEKPRGIFD"
gene <181403..>182548
/gene="RSA1"
/locus_tag="YPL193W"
/db_xref="GeneID:855908"
mRNA <181403..>182548
/gene="RSA1"
/locus_tag="YPL193W"
/product="Rsa1p"
/transcript_id="NM_001184007.1"
/db_xref="GeneID:855908"
CDS 181403..182548
/gene="RSA1"
/locus_tag="YPL193W"
/experiment="EXISTENCE:direct assay:GO:0005654 nucleoplasm
[PMID:10567587]"
/experiment="EXISTENCE:direct assay:GO:0034517 ribophagy
[PMID:40294649]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:40294649]"
/experiment="EXISTENCE:mutant phenotype:GO:0000027
ribosomal large subunit assembly [PMID:10567587]"
/experiment="EXISTENCE:mutant phenotype:GO:0000492 box C/D
snoRNP assembly [PMID:25170085]"
/note="Protein involved in the assembly of 60S ribosomal
subunits; functionally interacts with Dbp6p; functions in
a late nucleoplasmic step of the assembly"
/codon_start=1
/product="Rsa1p"
/protein_id="NP_015131.1"
/db_xref="GeneID:855908"
/db_xref="SGD:S000006114"
/translation="MNYNNFENSKGDGHSRLPKPTYSGTLSDGYDESKIKRQKTDSAF
NAAYSPHMYPNSPYYEGSWNTGYTPQLHHVAPHNQYFHPIQPSTQYNYTSPPNYTENY
IPPVHQNISYAPALNLQKWPSSYCENTQALKNDKDYQTSISYEDVAIPTVKEIQLIEK
NRGKDTFMNEISPVPSSKDQASAEPTEIPRKDPELANSNAEDDHNNLGLEDDDRDEQL
ESEGLGKVVLVPGTSIALITDEDVKKWREERKKMWLLKISNNKQKHMQEMGIKEDELK
SQPSIFKESRKEKQFIQSIQNQVQRGNPKIDLNLKLIQREFANENSQLLDFIRELGDV
GLLEYELSQQEKDVLFGSSEDNNKNHYKPNYKNRKPNLSRANFTRNK"
gene complement(<182655..>183056)
/gene="PRM3"
/locus_tag="YPL192C"
/db_xref="GeneID:855909"
mRNA complement(<182655..>183056)
/gene="PRM3"
/locus_tag="YPL192C"
/product="pheromone-regulated protein PRM3"
/transcript_id="NM_001184006.1"
/db_xref="GeneID:855909"
CDS complement(182655..183056)
/gene="PRM3"
/locus_tag="YPL192C"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:12514182]"
/experiment="EXISTENCE:direct assay:GO:0005816 spindle
pole body [PMID:19297527]"
/experiment="EXISTENCE:direct assay:GO:0034399 nuclear
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0000742
karyogamy involved in conjugation with cellular fusion
[PMID:19297527|PMID:12514182]"
/experiment="EXISTENCE:mutant phenotype:GO:0005635 nuclear
envelope [PMID:12514182]"
/experiment="EXISTENCE:mutant phenotype:GO:0048288 nuclear
membrane fusion involved in karyogamy [PMID:19297527]"
/note="Protein required for nuclear envelope fusion during
karyogamy; pheromone-regulated; peripheral protein of the
nuclear membrane; interacts with Kar5p at the spindle pole
body"
/codon_start=1
/product="pheromone-regulated protein PRM3"
/protein_id="NP_015132.1"
/db_xref="GeneID:855909"
/db_xref="SGD:S000006113"
/translation="MTAMKEDNAALITLKKNNDQEKLRVHKLTDASSNSADGFVINKA
KNGGPLNKKSLVNNEQHIKKAVSPGRVRKHKTTTSSTKSRTKSKKKDASESKVQRENK
GSFYQGAIFGSFLGAAVTTVLSNLAVKALQN"
gene complement(<183597..>184679)
/gene="MIY2"
/locus_tag="YPL191C"
/gene_synonym="MIY1"
/db_xref="GeneID:855910"
mRNA complement(<183597..>184679)
/gene="MIY2"
/locus_tag="YPL191C"
/gene_synonym="MIY1"
/product="ubiquitinyl hydrolase 1"
/transcript_id="NM_001184005.1"
/db_xref="GeneID:855910"
CDS complement(183597..184679)
/gene="MIY2"
/locus_tag="YPL191C"
/gene_synonym="MIY1"
/EC_number="3.4.19.12"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0071108 protein
K48-linked deubiquitination [PMID:27292798]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:1990380 K48-linked
deubiquitinase activity [PMID:27292798]"
/experiment="EXISTENCE:mutant phenotype:GO:0071444
cellular response to pheromone [PMID:39798877]"
/note="K48-specific deubiquitinating (DUB) enzyme; MINDY
family endo-type deubiquitinase that preferentially
cleaves long K48-linked polyubiquitin chains between
moieties; diploid deletion strain exhibits high budding
index; GFP-fusion protein localizes to the cytoplasm
endoplasmic reticulum and cell periphery in
high-throughput studies; YPL191C has a paralog, YGL082W,
that arose from the whole genome duplication; ortholog of
human MINDY2/FAM63B"
/codon_start=1
/product="ubiquitinyl hydrolase 1"
/protein_id="NP_015133.1"
/db_xref="GeneID:855910"
/db_xref="SGD:S000006112"
/translation="MDLSFTTKSVKINGQNHRILLQNENGPCALLALANILILSPDHT
RFSNELIRLVNKGSQISLKELIEVLADIALQVTDKPSTDISELLSLLPRLHEGLNINP
EFNGSFENTKEMSIFRLFNVDVVHGWVINSFINENIDEKLSHYSYESAQRILTQAADI
NCGISQDENSDEVLRDAMHLGLFLNESPTQLTAFGLLRLREKLLHNKFSILFRNDHFS
TLFKYEDRLYTLVTDFGYKNCKDIVWQSLDSVDGSCDAFFAGNFSAAEVNGQQLSTDI
ERDFGTGNLLLEEIQQIENDKELAKQLQEQEQERVTKFEAKRKIHSHKKNSEIHAPVK
KDKFKRRSSLLNAKASEKEKSECVVM"
gene complement(<185317..>187725)
/gene="NAB3"
/locus_tag="YPL190C"
/gene_synonym="HMD1"
/db_xref="GeneID:855911"
mRNA complement(<185317..>187725)
/gene="NAB3"
/locus_tag="YPL190C"
/gene_synonym="HMD1"
/product="Nab3p"
/transcript_id="NM_001184004.1"
/db_xref="GeneID:855911"
CDS complement(185317..187725)
/gene="NAB3"
/locus_tag="YPL190C"
/gene_synonym="HMD1"
/experiment="EXISTENCE:direct assay:GO:0001068
transcription regulatory region RNA binding
[PMID:21084293]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:31960028|PMID:20805243]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:7962083]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0030847 termination
of RNA polymerase II transcription, exosome-dependent
[PMID:16427013]"
/experiment="EXISTENCE:direct assay:GO:0035649 Nrd1
complex [PMID:10655211]"
/experiment="EXISTENCE:direct assay:GO:0071041 antisense
RNA transcript catabolic process [PMID:27190003]"
/experiment="EXISTENCE:mutant phenotype:GO:0031124 mRNA
3'-end processing [PMID:11565036]"
/experiment="EXISTENCE:mutant phenotype:GO:0031126
sno(s)RNA 3'-end processing [PMID:11565036]"
/experiment="EXISTENCE:mutant phenotype:GO:0034472 snRNA
3'-end processing [PMID:11565036]"
/experiment="EXISTENCE:mutant phenotype:GO:0042780 tRNA
3'-end processing [PMID:21460797]"
/experiment="EXISTENCE:mutant phenotype:GO:0071028 nuclear
mRNA surveillance [PMID:21113025]"
/experiment="EXISTENCE:mutant phenotype:GO:0071034 CUT
catabolic process [PMID:16973436]"
/experiment="EXISTENCE:mutant phenotype:GO:0071041
antisense RNA transcript catabolic process
[PMID:22028667]"
/note="RNA-binding protein, subunit of Nrd1 complex
(Nrd1p-Nab3p-Sen1p); complex interacts with exosome to
mediate 3'-end formation of some mRNAs, snRNAs, snoRNAs,
and CUTs; required for termination of non-poly(A)
transcripts and efficient splicing; Nrd1-Nab3 pathway
appears to have a role in rapid suppression of some genes
when cells are shifted to poor growth conditions,
indicating role for Nrd1-Nab3 in regulating cellular
response to nutrient availability"
/codon_start=1
/product="Nab3p"
/protein_id="NP_015134.1"
/db_xref="GeneID:855911"
/db_xref="SGD:S000006111"
/translation="MSDENHNSDVQDIPSPELSVDSNSNENELMNNSSADDGIEFDAP
EEEREAEREEENEEQHELEDVNDEEEEDKEEKGEENGEVINTEEEEEEEHQQKGGNDD
DDDDNEEEEEEEEDDDDDDDDDDDDEEEEEEEEEEGNDNSSVGSDSAAEDGEDEEDKK
DKTKDKEVELRRETLEKEQKDVDEAIKKITREENDNTHFPTNMENVNYDLLQKQVKYI
MDSNMLNLPQFQHLPQEEKMSAILAMLNSNSDTALSVPPHDSTISTTASASATSGARS
NDQRKPPLSDAQRRMRFPRADLSKPITEEEHDRYAAYLHGENKITEMHNIPPKSRLFI
GNLPLKNVSKEDLFRIFSPYGHIMQINIKNAFGFIQFDNPQSVRDAIECESQEMNFGK
KLILEVSSSNARPQFDHGDHGTNSSSTFISSAKRPFQTESGDMYNDDNGAGYKKSRRH
TVSCNIFVKRTADRTYAIEVFNRFRDGTGLETDMIFLKPRMELGKLINDAAYNGVWGV
VLVNKTHNVDVQTFYKGSQGETKFDEYISISADDAVAIFNNIKNNRNNSRPTDYRAMS
HQQNIYGAPPLPVPNGPAVGPPPQTNYYQGYSMPPPQQQQQQPYGNYGMPPPSHDQGY
GSQPPIPMNQSYGRYQTSIPPPPPQQQIPQGYGRYQAGPPPQPPSQTPMDQQQLLSAI
QNLPPNVVSNLLSMAQQQQQQPHAQQQLVGLIQSMQGQAPQQQQQQLGGYSSMNSSSP
PPMSTNYNGQNISAKPSAPPMSHQPPPPQQQQQQQQQQQQQQQQPAGNNVQSLLDSLA
KLQK"
gene complement(<188307..>188513)
/gene="COA2"
/locus_tag="YPL189C-A"
/db_xref="GeneID:1466518"
mRNA complement(<188307..>188513)
/gene="COA2"
/locus_tag="YPL189C-A"
/product="Coa2p"
/transcript_id="NM_001184537.1"
/db_xref="GeneID:1466518"
CDS complement(188307..188513)
/gene="COA2"
/locus_tag="YPL189C-A"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:18541668]"
/experiment="EXISTENCE:mutant phenotype:GO:0033617
mitochondrial respiratory chain complex IV assembly
[PMID:18541668]"
/experiment="EXISTENCE:physical interaction:GO:0033617
mitochondrial respiratory chain complex IV assembly
[PMID:18541668]"
/note="Cytochrome oxidase assembly factor; null mutation
results in respiratory deficiency with specific loss of
cytochrome oxidase activity; functions downstream of
assembly factors Mss51p and Coa1p and interacts with
assembly factor Shy1p"
/codon_start=1
/product="Coa2p"
/protein_id="NP_878179.1"
/db_xref="GeneID:1466518"
/db_xref="SGD:S000028527"
/translation="MRAVTRNKIVNNLYFSTFLIAFASVAIGSVLPCPAHSVDSDSPA
VQQHKLQLAHEQELKRKDALSKKI"
gene <189154..>190983
/gene="GUP2"
/locus_tag="YPL189W"
/db_xref="GeneID:855912"
mRNA <189154..>190983
/gene="GUP2"
/locus_tag="YPL189W"
/product="putative O-acyltransferase"
/transcript_id="NM_001184003.1"
/db_xref="GeneID:855912"
CDS 189154..190983
/gene="GUP2"
/locus_tag="YPL189W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0015793
glycerol transmembrane transport [PMID:10931309]"
/experiment="EXISTENCE:genetic interaction:GO:0071475
cellular hyperosmotic salinity response [PMID:10931309]"
/note="Probable membrane protein; possible role in proton
symport of glycerol; member of the MBOAT family of
putative membrane-bound O-acyltransferases; homolog of the
mammalian Hedgehog pathway modulator HHAT; GUP2 has a
paralog, GUP1, that arose from the whole genome
duplication"
/codon_start=1
/product="putative O-acyltransferase"
/protein_id="NP_015135.1"
/db_xref="GeneID:855912"
/db_xref="SGD:S000006110"
/translation="MSMLRIWSCIVHFFSVQALDSRIKPDIEFKRRQRIFINSSKEEN
GSSSSAVTVTRNPVLSSNSPSPPLWNTWEFRLYYLAFTVVVPFMIKAALATSSESNPN
YYKFSGLLAHGWILGRKVDNSDPQYRFFRSNFFLLAILILLQIILKKVFVKFSKIPKT
KFDFACGLVFVCFMYGINSVKLFTHAFIFFTLAHSLKRKRLIAAFAIWSYGIFTLFIN
QKMKNLPFNNIAIILSPMDQWYKGIVPRWDFFFNFTLLRLLSYSMDFLERWHEQLSRQ
PSIDYDDRRPEFRKSLSGSTLQTIYESGKNVLEEKERLVAEHHIQDYNFINFIAYITY
APLFLVGPIITFNDYLYQSENKLPSLTKKNIGFYALKVFSSLLLMEIILHYIYVGAIA
RTKAWNNDTPLQQAMIALFNLNIMYLKLLIPWRLFRLWAMVDGIDAPENMLRCVDNNY
STVGFWRAWHTSFNKWVIRYIYVPFGGSNNKILTSFAVFSFVAIWHDIQLRVLFWGWL
TVLLLLGETYITNCFSRYRFRSWYRFVCGIGAAINICMMMIINVYGFCLGAEGTKLLL
KGIFNNSHSPEFLTAVMVSLFIAVQVMFEIREEEKRHGINLKC"
gene <191406..>192650
/gene="POS5"
/locus_tag="YPL188W"
/db_xref="GeneID:855913"
mRNA <191406..>192650
/gene="POS5"
/locus_tag="YPL188W"
/product="NADH kinase"
/transcript_id="NM_001184002.1"
/db_xref="GeneID:855913"
CDS 191406..192650
/gene="POS5"
/locus_tag="YPL188W"
/EC_number="2.7.1.86"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:12727869]"
/experiment="EXISTENCE:direct assay:GO:0006741 NADP+
biosynthetic process [PMID:12727869]"
/experiment="EXISTENCE:direct assay:GO:0042736 NADH kinase
activity [PMID:12727869]"
/experiment="EXISTENCE:mutant phenotype:GO:0016226
iron-sulfur cluster assembly [PMID:20889970]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress [PMID:7586028]"
/note="Mitochondrial NADH kinase; preferentially
phosphorylates NADH; required for the response to
oxidative stress"
/codon_start=1
/product="NADH kinase"
/protein_id="NP_015136.1"
/db_xref="GeneID:855913"
/db_xref="SGD:S000006109"
/translation="MFVRVKLNKPVKWYRFYSTLDSHSLKLQSGSKFVKIKPVNNLRS
SSSADFVSPPNSKLQSLIWQNPLQNVYITKKPWTPSTREAMVEFITHLHESYPEVNVI
VQPDVAEEISQDFKSPLENDPNRPHILYTGPEQDIVNRTDLLVTLGGDGTILHGVSMF
GNTQVPPVLAFALGTLGFLSPFDFKEHKKVFQEVISSRAKCLHRTRLECHLKKKDSNS
SIVTHAMNDIFLHRGNSPHLTNLDIFIDGEFLTRTTADGVALATPTGSTAYSLSAGGS
IVSPLVPAILMTPICPRSLSFRPLILPHSSHIRIKIGSKLNQKPVNSVVKLSVDGIPQ
QDLDVGDEIYVINEVGTIYIDGTQLPTTRKTENDFNNSKKPKRSGIYCVAKTENDWIR
GINELLGFNSSFRLTKRQTDND"
gene <193648..>194145
/gene="MF(ALPHA)1"
/locus_tag="YPL187W"
/db_xref="GeneID:855914"
mRNA <193648..>194145
/gene="MF(ALPHA)1"
/locus_tag="YPL187W"
/product="Mf(Alpha)1p"
/transcript_id="NM_001184001.1"
/db_xref="GeneID:855914"
CDS 193648..194145
/gene="MF(ALPHA)1"
/locus_tag="YPL187W"
/experiment="EXISTENCE:direct assay:GO:0000772 mating
pheromone activity [PMID:6306574]"
/experiment="EXISTENCE:direct assay:GO:0005576
extracellular region [PMID:776628]"
/experiment="EXISTENCE:direct assay:GO:1903135 cupric ion
binding [PMID:27476515]"
/experiment="EXISTENCE:mutant phenotype:GO:0000750
pheromone-dependent signal transduction involved in
conjugation with cellular fusion [PMID:3887136]"
/experiment="EXISTENCE:mutant phenotype:GO:0000772 mating
pheromone activity [PMID:3887136]"
/note="Alpha-factor mating pheromone made by alpha cells;
interacts with mating type a cells to induce cell cycle
arrest and other responses leading to mating; also encoded
by MF(ALPHA)2, although MF(ALPHA)1 produces most
alpha-factor; binds copper(II) ions"
/codon_start=1
/product="Mf(Alpha)1p"
/protein_id="NP_015137.1"
/db_xref="GeneID:855914"
/db_xref="SGD:S000006108"
/translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYLDL
EGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLDKREAEAWHWLQLKPGQPMY
KREAEAEAWHWLQLKPGQPMYKREADAEAWHWLQLKPGQPMYKREADAEAWHWLQLKP
GQPMY"
gene complement(<194512..>195426)
/gene="UIP4"
/locus_tag="YPL186C"
/db_xref="GeneID:855916"
mRNA complement(<194512..>195426)
/gene="UIP4"
/locus_tag="YPL186C"
/product="Uip4p"
/transcript_id="NM_001184000.1"
/db_xref="GeneID:855916"
CDS complement(194512..195426)
/gene="UIP4"
/locus_tag="YPL186C"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:11056382]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:16407407]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762|PMID:11056382]"
/experiment="EXISTENCE:mutant phenotype:GO:0006998 nuclear
envelope organization [PMID:35171083]"
/note="Protein required for nuclear envelope integrity;
involved in distribution of nuclear pore complexes;
interacts with Ulp1p, a Ubl (ubiquitin-like
protein)-specific protease for Smt3p protein conjugates;
detected in a phosphorylated state in the mitochondrial
outer membrane; also detected in ER and nuclear envelope"
/codon_start=1
/product="Uip4p"
/protein_id="NP_015138.1"
/db_xref="GeneID:855916"
/db_xref="SGD:S000006107"
/translation="MVTIVFDHPAEDFPELKIAGEFTNWEGVPMKINTSSGKWEYKFD
ESSVTKHNDKDKVHFKFIDQNGNWFADDEYPKEVDEHSNENNVATLNNEEDGGSAGEE
KDEGDKTAHNTNENGSELYYEGPETPTPSLKGNVTFPSPKTAISQDGSAFAKETTRKE
RKYEHAPLNEVPVERDPKEENKELSPNFSQEQTENKQDKGLDNLSEGNDNDNTRVNED
TDVTDTQESEHEINGSDTENTDMSEQEEIQKIDKPADQNAKSIVKEGDANTEDYESVL
KKLLGALGRFFGSWFSWLTTKMSSSEAS"
gene complement(<195950..>197788)
/gene="MRN1"
/locus_tag="YPL184C"
/gene_synonym="PTR69"
/db_xref="GeneID:855917"
mRNA complement(<195950..>197788)
/gene="MRN1"
/locus_tag="YPL184C"
/gene_synonym="PTR69"
/product="Mrn1p"
/transcript_id="NM_001183998.1"
/db_xref="GeneID:855917"
CDS complement(195950..197788)
/gene="MRN1"
/locus_tag="YPL184C"
/gene_synonym="PTR69"
/experiment="EXISTENCE:direct assay:GO:0000932 P-body
[PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0003730 mRNA 3'-UTR
binding [PMID:36862555]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:23028530]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:23028530]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405]"
/experiment="EXISTENCE:genetic interaction:GO:0000398 mRNA
splicing, via spliceosome [PMID:23028530]"
/experiment="EXISTENCE:genetic interaction:GO:0006338
chromatin remodeling [PMID:23028530]"
/experiment="EXISTENCE:genetic interaction:GO:0061158
3'-UTR-mediated mRNA destabilization [PMID:36862555]"
/experiment="EXISTENCE:genetic interaction:GO:1990394
cellular response to cell wall damage [PMID:36862555]"
/experiment="EXISTENCE:mutant phenotype:GO:0061158
3'-UTR-mediated mRNA destabilization [PMID:36862555]"
/experiment="EXISTENCE:mutant phenotype:GO:1990394
cellular response to cell wall damage [PMID:36862555]"
/note="mRNA-binding protein; binds to the 3'UTR of cell
wall-related mRNAs and destabilizes these mRNAs during
stress, acting antagonistically to Nab6p; links cell wall
homeostasis to mitochondrial structure and function; binds
specific categories of mRNAs, including those that contain
upstream open reading frames (uORFs) and internal ribosome
entry sites (IRES); interacts genetically with chromatin
remodelers and splicing factors, linking chromatin state,
splicing and as a result mRNA maturation"
/codon_start=1
/product="Mrn1p"
/protein_id="NP_015140.1"
/db_xref="GeneID:855917"
/db_xref="SGD:S000006105"
/translation="MVVSYNNNNNNNNNNNNNNISNNNNNNNMFPPFPSSDDFAMYQQ
SSSSGPYQETYASGPQNFGDAVYPMNGNFTLLPSDFTREPNDSFFYENDGIFDYQRIQ
QQPTQFQTKQRNDSQQQRFSQEQNFEIDNEVVHNNNRYYEYERSSNEVSPFDDENPNV
LSDGMSPTIMATATAVTNANAPLPVNAQANNPLNFTSAPSRTVYLGNVPPNLSVKELL
DHVRSGVVEDVKIIPEKMCAFVSFIDESAALLFHSDAILKRLNIGDRDIKIGWGKPTR
IDPIVAARISTDGATRNVYIGRMTIEGEESHLSEEQLRVDLEEYGEIDCIKIIKEKGI
AFIHFASILNAIKVVTNLPIRNPYYQNKRIFYGKDRCAFITKTQQHNAAQFLGVQPGM
EHMIEFSDREFISNALLQQSAAAAAIATSAGGPNNLGNRTVYLGSLPKDVKIEEICNA
VRGGLLQSIKLLNDRYVCFVTFIDPTAAAQFYAMSSLYGFTVQKKRCKVGWGKHSGPL
PNALALAVSNGASRNVYVGNIDFVGDSLRDERVFTESNLRHIFQQYGEVEQINFLPEK
NCCFINYTNISNAILALDKIKSNPYFKDLKINFGKDRCGNVPHQSR"
gene <199095..>199376
/gene="RTC6"
/locus_tag="YPL183W-A"
/gene_synonym="bL36m; TAE4"
/db_xref="GeneID:855918"
mRNA <199095..>199376
/gene="RTC6"
/locus_tag="YPL183W-A"
/gene_synonym="bL36m; TAE4"
/product="mitochondrial 54S ribosomal protein bL36m RTC6"
/transcript_id="NM_001184317.1"
/db_xref="GeneID:855918"
CDS 199095..199376
/gene="RTC6"
/locus_tag="YPL183W-A"
/gene_synonym="bL36m; TAE4"
/experiment="EXISTENCE:direct assay:GO:0008270 zinc ion
binding [PMID:38493895]"
/experiment="EXISTENCE:genetic interaction:GO:0042254
ribosome biogenesis [PMID:20691087]"
/experiment="EXISTENCE:mutant phenotype:GO:0042254
ribosome biogenesis [PMID:20691087]"
/note="Protein involved in translation; mutants have
defects in biogenesis of nuclear ribosomes; sequence
similar to prokaryotic ribosomal protein L36, may be a
mitochondrial ribosomal protein; protein abundance
increases in response to DNA replication stress"
/codon_start=1
/product="mitochondrial 54S ribosomal protein bL36m RTC6"
/protein_id="NP_015141.1"
/db_xref="GeneID:855918"
/db_xref="SGD:S000007224"
/translation="MFLQTLRLTMPRMFLHMKPSPITITRACTVPSLLSVAAPQPALV
AANRPLVFNRGFKVRTSVKKFCSDCYLVRRKGRVYIYCKSNKKHKQRQG"
gene complement(<199495..>202536)
/gene="RTT10"
/locus_tag="YPL183C"
/gene_synonym="ERE2; TRM734"
/db_xref="GeneID:855919"
mRNA complement(<199495..>202536)
/gene="RTT10"
/locus_tag="YPL183C"
/gene_synonym="ERE2; TRM734"
/product="tRNA (34-2'-O)-methyltransferase regulator
RTT10"
/transcript_id="NM_001183997.1"
/db_xref="GeneID:855919"
CDS complement(199495..202536)
/gene="RTT10"
/locus_tag="YPL183C"
/gene_synonym="ERE2; TRM734"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:21880895|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:21880895]"
/experiment="EXISTENCE:mutant phenotype:GO:0002130 wobble
position ribose methylation [PMID:22912484]"
/experiment="EXISTENCE:mutant phenotype:GO:0032456
endocytic recycling [PMID:21880895]"
/experiment="EXISTENCE:physical interaction:GO:0002130
wobble position ribose methylation [PMID:22912484]"
/note="WD40 domain-containing protein involved in
endosomal recycling; forms a complex with Rrt2p that
functions in the retromer-mediated pathway for recycling
internalized cell-surface proteins; interacts with Trm7p
for 2'-O-methylation of N34 of substrate tRNAs; has a role
in regulation of Ty1 transposition; human ortholog is
WDR6"
/codon_start=1
/product="tRNA (34-2'-O)-methyltransferase regulator
RTT10"
/protein_id="NP_015142.1"
/db_xref="GeneID:855919"
/db_xref="SGD:S000006104"
/translation="MKDLSHYGPALCVKFYNDYVLAGYGPFIHVYDYHSATLINKCRL
FHYNKVHGLSLSSEGKILAYGARSVTIVELEDVLKKESLVDFERINSDWITGATFSFD
NLQIYLLTCYNKVLICDLNCEVLFRKSLGGERSILYSGIIKVFGPDKVYVNAGTVMGG
VIIWDLFSETKIHNLLGHEGSIFYVNLSNNGRYVASCSDDRSIRLWDLETGKQLSVGW
SHTARIWNLMFFDNDSKLISVSEDCTCRVWNIIESRENVAELSISNVYEVHLIKSIWG
VDVKDDEMIAVTSGNDGRLKLIDLLQLKRHGDEETSFSLDDIAKQCGDIFEKNESIKG
FQWFSFGVIAITSLGKILKYSDVTKQWKLLLTNEKFNSYPITNGIQTQNIAVFSNNKS
DILLIKFSKDSADIIETEEFHLDELSKTNNCLVTEYDDDSFLLTLQSPNPREKFVCLE
ISLQNLKIKSKHCFNKPENFSSSCLTSFRNHILVGSRFSTLVIYNLLDESEEPFIIRR
LSPGDTTTSIEFVEDKDNSAVFSVTNRDGYYVFIELTKNSLEEGPYRLSYKVLHSNKM
MKGFLEGAFFNSKGEYITYGFKSSLFYLYNETNCYELASEVCGGSHRLWNLAKITDGH
VLMYIKASRFHLRKIYNSIVPETLENGVHGREIRDISICPVSNTNTNDNFKDGHIFCT
ASEDTTIKLGYFNNRTGKVQNFWTQRKHVSGLQRCQFINHKLMISSSAREELFLWELN
DKYNKRPYMTIRQALPVSTNNSDLRIMDFDVKFISQSGDFLLVTVYSDSTIKIWHYRE
NQNKFDLIMQGRYKTCCLFNVVFIALKEELLVVISPTDGHLVVYNITEYVPFSVDPIS
GDLVDHKLDATISNLPAPVAQLPVHQSGVKSLDYVANATRTSATILTGGDDNGLGLSN
LKLDDSNKVTLKTSDFIAAAASSTITSGMLINGGKEVITTSVDQVIRAWEITAGKLSL
VDKKRTTVADTGSLEIISNDEDADSEKTLLIGGVGLSIWKK"
gene <203421..>204941
/gene="CTI6"
/locus_tag="YPL181W"
/gene_synonym="RXT1"
/db_xref="GeneID:855920"
mRNA <203421..>204941
/gene="CTI6"
/locus_tag="YPL181W"
/gene_synonym="RXT1"
/product="Cti6p"
/transcript_id="NM_001183995.1"
/db_xref="GeneID:855920"
CDS 203421..204941
/gene="CTI6"
/locus_tag="YPL181W"
/gene_synonym="RXT1"
/experiment="EXISTENCE:direct assay:GO:0033698 Rpd3L
complex
[PMID:16286007|PMID:19040720|PMID:16286008|PMID:16314178]"
/experiment="EXISTENCE:direct assay:GO:0070210
Rpd3L-Expanded complex [PMID:19040720]"
/experiment="EXISTENCE:direct assay:GO:0140002 histone
H3K4me3 reader activity [PMID:17142463]"
/experiment="EXISTENCE:mutant phenotype:GO:0030174
regulation of DNA-templated DNA replication initiation
[PMID:19417103]"
/experiment="EXISTENCE:mutant phenotype:GO:0045893
positive regulation of DNA-templated transcription
[PMID:12086626]"
/experiment="EXISTENCE:mutant phenotype:GO:0061186
negative regulation of silent mating-type cassette
heterochromatin formation [PMID:16286008]"
/experiment="EXISTENCE:mutant phenotype:GO:0061188
negative regulation of rDNA heterochromatin formation
[PMID:16286008]"
/note="Component of the Rpd3L histone deacetylase complex;
relieves transcriptional repression by binding to the
Cyc8p-Tup1p corepressor and recruiting the SAGA complex to
the repressed promoter; contains a PHD finger domain"
/codon_start=1
/product="Cti6p"
/protein_id="NP_015144.1"
/db_xref="GeneID:855920"
/db_xref="SGD:S000006102"
/translation="MESTAIVPKGPVVGSEDMEKAEVIASGSTDIISTTSTATTTAAI
GSVQEESVKQEDVPMEGGEGEVEEEEGETRCICGELDTPDDSGFFIQCEQCSSWQHGY
CVSITQDNAPDKYWCEQCRPELHQLFTTDTGEARSIYKPVQEKRRQSRRKARSAAASK
SHAANEAEKSPRNTSNTDDNVDDIGDEEDEVEDEASAVALAKDGNTRSSRRRRRNSMD
DASTDQYSLDPGDSDKKLLDRKRATFMAREEKQYQRMLEKALKESRRTSHQEDPESYE
NDADIYQGDTDNHNGTTRLQTDVMLTEGKPDSVTNDDMKESLRPSKEQSMEKTNDVEK
EASQEKESSTGSAQDTEKTDEPILPLTSISSSEDDSRKASSRGSKRVSKPARKGNRTR
RSNTSSDTNQNRRSADIGTDKPVKPRLPPQRTSLNEMRRRVSAILEFISRTQWELSED
QSDREEFVRFVENQHFVEKVDTIYNGYNESLSMMDDLTRELLLWEKKYSNNTNAIQ"
gene <205248..>207647
/gene="TCO89"
/locus_tag="YPL180W"
/db_xref="GeneID:855922"
mRNA <205248..>207647
/gene="TCO89"
/locus_tag="YPL180W"
/product="Tco89p"
/transcript_id="NM_001183994.1"
/db_xref="GeneID:855922"
CDS 205248..207647
/gene="TCO89"
/locus_tag="YPL180W"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane
[PMID:19748353|PMID:14562095|PMID:17560372|PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0006071
glycerol metabolic process [PMID:10931309]"
/experiment="EXISTENCE:mutant phenotype:GO:0009651
response to salt stress [PMID:17298473]"
/experiment="EXISTENCE:mutant phenotype:GO:0031505
fungal-type cell wall organization [PMID:14736892]"
/experiment="EXISTENCE:physical interaction:GO:0001558
regulation of cell growth [PMID:14736892]"
/experiment="EXISTENCE:physical interaction:GO:0031931
TORC1 complex [PMID:14736892]"
/note="Subunit of TORC1 (Tor1p or
Tor2p-Kog1p-Lst8p-Tco89p); regulates global H3K56ac; TORC1
complex regulates growth in response to nutrient
availability; cooperates with Ssd1p in the maintenance of
cellular integrity; deletion strains are hypersensitive to
rapamycin"
/codon_start=1
/product="Tco89p"
/protein_id="NP_015145.1"
/db_xref="GeneID:855922"
/db_xref="SGD:S000006101"
/translation="MVHRGRTLKSDTDVTSLNASTVSHQSKPFRQFSTRSRAKSNASF
KGLRRVLTHDGTLDNDYFNKHNVSQKCKSSDALFRKRTISGLNMTALTRVKSNQGKRS
ASFHSPVHNTLLSPKNSSHSNTGTAGFGLKPRRSKSTQSVLSLRDAQESKKSESTTDE
EVECFSEDNIEDGKVNNDKVIAEHVMPEEKKNVQQLNQNELQSPDSIDEQEEDKSGTD
GKENHRAVSLPLPHLSSNNYFGESSHSIEHQKDGETSPSSIETKLNATSVINEEGQSK
VTKEADIDDLSSHSQNLRASLVKAGDNISEAPYDKEKKILDVGNTLAAHKSNQKPSHS
DEQFDQEDHIDAPRSNSSRKSDSSFMSLRRQSSKQHKLLNEEEDLIKPDDISSAGTKD
IEGHSLLENYAPNMILSQSTGVERRFENSSSIQNSLGNEIHDSGEHMASGDTFNELDD
GKLRKSKKNGGRSQLGQNIPNSQSTFPTIANIGSKDNNVPQHNFSTSISSLTNNLRRA
APESFHGSRMNNIFHKKGNQNLLLRSNDLNKNSAAPASPLSNEHITSSTNSGSDANRQ
SNSGAKFNSFAQFLKSDGIDAESRTQRKLWLQRENSIMDLSSQNDGSDSIFMAGNIDA
KREFERISHEYSNVKRFYNPLDEALLRVQPIITGNANNIRKKSHNDAQSIAHSSSDTD
HKDEDDLLFTNYDKKFDDLYPHLASAKIQAVLSGIWKSESYLFNKDVNPINKNRTTST
NHSVGHTASQNARNLLRGPMGSSTTLHHQRVINSLQPTTRAVNRRMENVGYMHTQPQQ
R"
gene <208157..>209806
/gene="PPQ1"
/locus_tag="YPL179W"
/gene_synonym="SAL6"
/db_xref="GeneID:855923"
mRNA <208157..>209806
/gene="PPQ1"
/locus_tag="YPL179W"
/gene_synonym="SAL6"
/product="protein-serine/threonine phosphatase"
/transcript_id="NM_001183993.1"
/db_xref="GeneID:855923"
CDS 208157..209806
/gene="PPQ1"
/locus_tag="YPL179W"
/gene_synonym="SAL6"
/EC_number="3.1.3.16"
/experiment="EXISTENCE:direct assay:GO:0004721
phosphoprotein phosphatase activity [PMID:24309106]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0090029
negative regulation of pheromone-dependent signal
transduction involved in conjugation with cellular fusion
[PMID:24309106]"
/note="Protein phosphatase that regulates the mating
response; negatively regulates the MAP kinase signaling
cascade during mating; member of the serine/threonine
phosphatase PP1 family"
/codon_start=1
/product="protein-serine/threonine phosphatase"
/protein_id="NP_015146.1"
/db_xref="GeneID:855923"
/db_xref="SGD:S000006100"
/translation="MRRSPSRSNNNFAVPNCSTNSNSSQQQLTTPSDDLNSNEPNDPD
DSRSLPTIKKFNNKHSINNYNTLASAGKNNNNKRASNDNLLIPGENAHKQKIYTKDEN
LKSLYLDIDVSVAKALSSSATAPKLINTARTSSTTTATTSNNILTSPSYRESNYSSPS
SYSFSSYYSSATSASSSTSSFLKSSGLSSRVKSPSSSVKAGSFGAPSSPTSGIPNPKS
SKKPIFLRRYSHDTSSNEGLDIDVAIEKLLQVGESREITKTSKKKNFPFHSWEIQLIC
YHAREIFLNQPTLLRLQAPIKVVGDVHGQFNDLLRILKLSGVPSDTNYLFLGDYVDRG
KNSLETILLLLCYKIKYKDNFFMLRGNHESANVTKMYGFYDECKRRLSSKVWKMFVDV
FNTLPLAAIIQDKIFCVHGGISPDLHDMKQIEKVARPTDIPESGLVTDLLWSDPDPQV
TDWSENDRGVSYTFSKRNVLDFCAKFKFDLILRGHMVVEDGYEFFARKKFVTIFSAPN
YCGEFHNWGAVMSVTTGMMCSFELLKPRALKNKKKLYKTKV"
gene complement(210192..210263)
/locus_tag="YNCP0004C"
/db_xref="GeneID:855924"
tRNA complement(210192..210263)
/locus_tag="YNCP0004C"
/product="tRNA-Glu"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE
analysis; thiolation of uridine at wobble position (34)
requires Ncs6p; target of K. lactis zymocin"
/db_xref="GeneID:855924"
/db_xref="SGD:S000006561"
repeat_region 210451..210771
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007194"
repeat_region 211577..211898
/note="Ty2 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007195"
gene <212158..>212784
/gene="CBC2"
/locus_tag="YPL178W"
/gene_synonym="CBP20; MUD13; SAE1"
/db_xref="GeneID:855925"
mRNA <212158..>212784
/gene="CBC2"
/locus_tag="YPL178W"
/gene_synonym="CBP20; MUD13; SAE1"
/product="nuclear cap-binding protein subunit CBC2"
/transcript_id="NM_001183992.1"
/db_xref="GeneID:855925"
CDS 212158..212784
/gene="CBC2"
/locus_tag="YPL178W"
/gene_synonym="CBP20; MUD13; SAE1"
/experiment="EXISTENCE:direct assay:GO:0000243 commitment
complex [PMID:8682299]"
/experiment="EXISTENCE:direct assay:GO:0000339 RNA cap
binding [PMID:8682299]"
/experiment="EXISTENCE:direct assay:GO:0005846 nuclear cap
binding complex [PMID:8811086]"
/experiment="EXISTENCE:mutant phenotype:GO:0000398 mRNA
splicing, via spliceosome [PMID:8682299]"
/note="Small subunit of the heterodimeric cap binding
complex with Sto1p; interacts with Npl3p, possibly to
package mRNA for export from the nucleus; may have a role
in telomere maintenance; contains an RNA-binding motif"
/codon_start=1
/product="nuclear cap-binding protein subunit CBC2"
/protein_id="NP_015147.1"
/db_xref="GeneID:855925"
/db_xref="SGD:S000006099"
/translation="MSLEEFDEVKYDHSTKRLDTPSRYLLRKARRNPNGLQELRESMK
SSTIYVGNLSFYTSEEQIYELFSKCGTIKRIIMGLDRFKFTPCGFCFIIYSCPDEALN
ALKYLSDTKLDEKTITIDLDPGFEDGRQFGRGKSGGQVSDELRFDFDASRGGFAIPFA
ERVGVPHSRFDNSSSQSNTNNYIPPPDAMGTFRPGFDEEREDDNYVPQ"
gene complement(<213042..>213962)
/gene="CUP9"
/locus_tag="YPL177C"
/db_xref="GeneID:855926"
mRNA complement(<213042..>213962)
/gene="CUP9"
/locus_tag="YPL177C"
/product="Cup9p"
/transcript_id="NM_001183991.1"
/db_xref="GeneID:855926"
CDS complement(213042..213962)
/gene="CUP9"
/locus_tag="YPL177C"
/experiment="EXISTENCE:curator inference:GO:0005634
nucleus [PMID:9427760]"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:9427760]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:19158363]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:9427760|PMID:17005992|PMID:18708352|PMID:10850718]"
/experiment="EXISTENCE:mutant phenotype:GO:2000877
negative regulation of oligopeptide transport
[PMID:17005992]"
/experiment="EXISTENCE:mutant phenotype:GO:2000879
negative regulation of dipeptide transport
[PMID:9427760|PMID:17005992|PMID:10850718|PMID:18708352]"
/experiment="EXISTENCE:physical interaction:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:18708352]"
/note="Homeodomain-containing transcriptional repressor;
regulates expression of PTR2, which encodes a major
peptide transporter; imported peptides activate
ubiquitin-dependent proteolysis, resulting in degradation
of Cup9p and de-repression of PTR2 transcription; CUP9 has
a paralog, TOS8, that arose from the whole genome
duplication; protein abundance increases in response to
DNA replication stress"
/codon_start=1
/product="Cup9p"
/protein_id="NP_015148.1"
/db_xref="GeneID:855926"
/db_xref="SGD:S000006098"
/translation="MNYNCEIQNRNSKNVDNQVSLPPIQVLFNSIEKRSMPELAFSNI
EYSHGNLRSSTEEQNYPAPVLLPQHHSIAYPAINSGGTSTTATPTASTVETSKTSSSA
MDTQSQYGSSKKSKSASDDAKPCYKSAPIYEIINKEKDAGAQYNRPFSDFVESKSRRK
QNSGRRSNLPKETVQILNTWLLNHLNNPYPTQQEKRELLIKTGLTKIQLSNWFINVRR
RKIFSDYYTLVNSIPNDNANNTPVERVQNVSAYHNTLSATNNTMYDATSTCSTDYELS
KRFAHAPVTRRKKLIDRLEELKKLSNPDMN"
gene complement(<216012..>218363)
/gene="TRE1"
/locus_tag="YPL176C"
/db_xref="GeneID:855927"
mRNA complement(<216012..>218363)
/gene="TRE1"
/locus_tag="YPL176C"
/product="Tre1p"
/transcript_id="NM_001183990.1"
/db_xref="GeneID:855927"
CDS complement(216012..218363)
/gene="TRE1"
/locus_tag="YPL176C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:16456538|PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0043328
protein transport to vacuole involved in
ubiquitin-dependent protein catabolic process via the
multivesicular body sorting pathway [PMID:19369420]"
/note="Transferrin receptor-like protein; plasma membrane
protein that binds Bsd2p and regulates ubiquitination and
vacuolar degradation of the metal transporter Smf1p;
functionally redundant with Tre2p; TRE1 has a paralog,
TRE2, that arose from the whole genome duplication"
/codon_start=1
/product="Tre1p"
/protein_id="NP_015149.1"
/db_xref="GeneID:855927"
/db_xref="SGD:S000006097"
/translation="MVNTRGYTTLPNVEEPANNSQDELNSQDFEQAIGMPSEPPVYVE
EMGMEEPQAPEAFSEKVQRFRMCFENNVVIPVKKNVVDPLAQMISLASEKFDLFLSKI
GNVMVMRRIFYIMMMSIIAALIIASDRLPNGKARGSNGSFSDHDLLLQYARKSIDLSK
IERDLEYISSMPHMSGTSGDAAIRHYIKESFDKNGIRLAGEEEFMAYSNYPGNVSLRV
YSKDDTEGFDIPLNEENFNPMSHNGQLNNIPVIYANKASLDDMASMQDQGLLNGDFIL
LVHYGDYVFQQMLTAQEYGAKAIIFISEPYQDNKDVIQMKSVALPQYGTGDALTPEWE
GSIRDPIDATEAKCLPKIPSIPISANQGDKILAILSDTGVKFSNNLFSGSLNDCRLDL
LVQTAIRERHPVHDIVGKIEGSEQAGRAIVIAAPRNSASYGTMYPSFGTVVLLSLIQL
YQEMVYKFDWKPLRNIYFISFGGSEFNEAGATELMEKRTEALKSEIYTIIDVGQIGIW
DDSNNLEIQCHPLLVDLFQKNMTSRKFNVKVDNVHQFGDWTPYLAQGIPVAIISSPGV
MNREHPIYTVEDKFDFIKDKLRDKKKGEVLSEIMLYLVEKSLELIDDPFIPFSISNYV
DFLSTTLKDLQKECPDTVNFDEVFLGTTLWENTKLQFEKWKSEWTELMYGAGTYIEPT
IIAINRWSWNYLLSLIGVTQCLEEGLMDRTFYKNVIFGPKLWVDKGDPLRSWTFPEIR
DTIAIKDWSSVQVQANTLGTILQNTARYFLENKNLHGINTNEF"
gene <218630..>220088
/gene="SPT14"
/locus_tag="YPL175W"
/gene_synonym="CWH6; GPI3"
/db_xref="GeneID:855928"
mRNA join(<218630..218646,218747..>220088)
/gene="SPT14"
/locus_tag="YPL175W"
/gene_synonym="CWH6; GPI3"
/product="phosphatidylinositol
N-acetylglucosaminyltransferase SPT14"
/transcript_id="NM_001183989.1"
/db_xref="GeneID:855928"
CDS join(218630..218646,218747..220088)
/gene="SPT14"
/locus_tag="YPL175W"
/gene_synonym="CWH6; GPI3"
/EC_number="2.4.1.198"
/experiment="EXISTENCE:curator inference:GO:0006506 GPI
anchor biosynthetic process [PMID:10970797]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0005783
endoplasmic reticulum [PMID:9079905]"
/experiment="EXISTENCE:mutant phenotype:GO:0017176
phosphatidylinositol N-acetylglucosaminyltransferase
activity [PMID:10970797]"
/note="UDP-glycosyltransferase subunit of the GPI-GnT
complex; UDP-GlcNAc-binding and catalytic subunit of the
enzyme that mediates the first step in
glycosylphosphatidylinositol (GPI) biosynthesis, mutations
cause defects in transcription and in biogenesis of cell
wall proteins"
/codon_start=1
/product="phosphatidylinositol
N-acetylglucosaminyltransferase SPT14"
/protein_id="NP_015150.2"
/db_xref="GeneID:855928"
/db_xref="SGD:S000006096"
/translation="MGFNIAMLCDFFYPQLGGVEFHIYHLSQKLIDLGHSVVIITHAY
KDRVGVRHLTNGLKVYHVPFFVIFRETTFPTVFSTFPIIRNILLREQIQIVHSHGSAS
TFAHEGILHANTMGLRTVFTDHSLYGFNNLTSIWVNKLLTFTLTNIDRVICVSNTCKE
NMIVRTELSPDIISVIPNAVVSEDFKPRDPTGGTKRKQSRDKIVIVVIGRLFPNKGSD
LLTRIIPKVCSSHEDVEFIVAGDGPKFIDFQQMIESHRLQKRVQLLGSVPHEKVRDVL
CQGDIYLHASLTEAFGTILVEAASCNLLIVTTQVGGIPEVLPNEMTVYAEQTSVSDLV
QATNKAINIIRSKALDTSSFHDSVSKMYDWMDVAKRTVEIYTNISSTSSADDKDWMKM
VANLYKRDGIWAKHLYLLCGIVEYMLFFLLEWLYPRDEIDLAPKWPKKTVSNETKEAR
ET"
rep_origin 220088..220167
/note="ARS1611; Putative replication origin; identified in
multiple array studies, not yet confirmed by plasmid-based
assay"
/db_xref="SGD:S000130197"
gene complement(<220167..>222773)
/gene="NIP100"
/locus_tag="YPL174C"
/gene_synonym="PAC13"
/db_xref="GeneID:855929"
mRNA complement(<220167..>222773)
/gene="NIP100"
/locus_tag="YPL174C"
/gene_synonym="PAC13"
/product="Nip100p"
/transcript_id="NM_001183988.1"
/db_xref="GeneID:855929"
CDS complement(220167..222773)
/gene="NIP100"
/locus_tag="YPL174C"
/gene_synonym="PAC13"
/experiment="EXISTENCE:direct assay:GO:0005869 dynactin
complex [PMID:9658168]"
/experiment="EXISTENCE:mutant phenotype:GO:0000132
establishment of mitotic spindle orientation
[PMID:9658168]"
/note="Large subunit of the dynactin complex; dynactin is
involved in partitioning the mitotic spindle between
mother and daughter cells; putative ortholog of mammalian
p150(glued)"
/codon_start=1
/product="Nip100p"
/protein_id="NP_015151.1"
/db_xref="GeneID:855929"
/db_xref="SGD:S000006095"
/translation="MRNAGVQVDTNMQKISLQDTVLVNEMKGRVKFIGETQFAKGIWY
GIELDKPLGKNDGSANGIRYFDIDLKKANSNGGYYGLFCKKDTLQFYKPDDDEHSLLN
GNAAQETIKNLQVKCESLASKLNKIKIENHELKTSVEKLSTNETVLLSKISRLDKLVK
ELKVENGNMKTHLDNFNHLLDASDSVMAPDLDKGTLLERSHLLQGLLDQTKLSYDKAM
KVQEDLLEENTQLLEENAVLSKKISDLGLQLQQTNNTIGDLALQIEAQSKSSNIVDKL
TNDNILLTSNIKALNNELEELQAKEKLDENLRITYEQLEQELRLQLSNLQSALENEKE
IAGTYIEENSRLKATLESIEAKTSHKFQSLELKVNTLQEELYQNKLLKKFYQIYEPFA
QPHLAALSSQLQYLAEVIESENFGKLENIEIHIILKVLSSISYALHIYTIKNTPDHLE
TTLQCFKVNIAPISMWLSEFLQRKFSSKQETAFSICQFLEDNKFLDKDVTLILKILHP
ILETTVPKLLAFLRTNSNFNDNDTLCLIGSLYERSLSLIARIDKLIGKEEISKQDNRL
FLYPSCDITLSSILTILFSDALFLRQDYKRISSLKKLEVFFQGIESLLENITIFPEQP
SQQTSDSESQCNIKEGNFSNSLLSDRLNEENIRLKEVLVQKENMLTELETKIKIIIGR
DLERKTLEENIKTLKVELNNKNEENCGKTEILNKLKEENFNLVNRLKNMELKLYQIKD
NNTLNKIYLDREKVDRVNLVSEIMELRETIRRQIKEQKRVSIDFSWLDELPAVENKQP
FKEHINHSLDTLGIEMFNFVSTSRILDLKLDQPLAEDELWHERDHSYISYLKRKRKNI
RLKSQNVVTYYK"
gene <223143..>224036
/gene="MRPL40"
/locus_tag="YPL173W"
/gene_synonym="uL24m"
/db_xref="GeneID:855930"
mRNA <223143..>224036
/gene="MRPL40"
/locus_tag="YPL173W"
/gene_synonym="uL24m"
/product="mitochondrial 54S ribosomal protein uL24m
MRPL40"
/transcript_id="NM_001183987.1"
/db_xref="GeneID:855930"
CDS 223143..224036
/gene="MRPL40"
/locus_tag="YPL173W"
/gene_synonym="uL24m"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:12392552|PMID:9151978]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:9151978|PMID:12392552]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005762
mitochondrial large ribosomal subunit
[PMID:12392552|PMID:9151978]"
/note="Mitochondrial ribosomal protein of the large
subunit"
/codon_start=1
/product="mitochondrial 54S ribosomal protein uL24m
MRPL40"
/protein_id="NP_015152.1"
/db_xref="GeneID:855930"
/db_xref="SGD:S000006094"
/translation="MSGSYQHLSNVGSRVMKRLGNRPKNFLPHSEKFIKKSTPEFMKS
DLKEVDEKTSFKSEKEWKFIPGDRVVVMSGASKGNIAVIKSFDKRTNSFILDENGPTK
TVPVPKQFWLEGQTSHMITIPVSILGKDLRLVADIDDEKTPGKTRTVAVRDVSFNGSY
YDADYKKVMPYRCVKGQPDLIIPWPKPDPIDVQTNLATDPVIAREQTFWVDSVVRNPI
PKKAIPSIRNPHSKYKRGTLTAKDIAKLVAPEMPLTEVRKSHLAEKKELAEREVPKLT
EEDMEAIGARVFEFLEKQKRE"
gene complement(<224353..>225741)
/gene="COX10"
/locus_tag="YPL172C"
/db_xref="GeneID:855931"
mRNA complement(<224353..>225741)
/gene="COX10"
/locus_tag="YPL172C"
/product="protoheme IX farnesyltransferase"
/transcript_id="NM_001183986.1"
/db_xref="GeneID:855931"
CDS complement(224353..225741)
/gene="COX10"
/locus_tag="YPL172C"
/EC_number="2.5.1.141"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:mutant phenotype:GO:0006784 heme A
biosynthetic process [PMID:8118433]"
/note="Heme A:farnesyltransferase; catalyzes first step in
conversion of protoheme to heme A prosthetic group
required for cytochrome c oxidase activity; human ortholog
COX10 can complement yeast cox10 null mutant; human
ortholog COX10 is associated with mitochondrial disorders"
/codon_start=1
/product="protoheme IX farnesyltransferase"
/protein_id="NP_015153.1"
/db_xref="GeneID:855931"
/db_xref="SGD:S000006093"
/translation="MSYFPRTYAHLMRNVLAHNKGNIYLQIGTQLHDTQIKIRFNGVR
YISRNHGGKQQHINTAPIEFTPNFGYGDRTSNCNKKVESTAMKTLRCTDDISTSSGSE
ATTDASTQLPFNVKLVDPMVRKSKRPSHAISEGLNMKTLKKKVIMPYLQLTKPRLTIL
VMLSAICSYALSPYPASVNELLCLTVGTTLCSGSANAINMGREPEFDRQMVRTQARPV
VRGDVTPTQAFEFAALIGTLGVSILYFGVNPTVAILGASNIALYGWAYTSMKRKHIIN
TWLGALVGMVPPLMGWAAASPLSHPGSWCLAGLLFAWQFPHFNTLSHNIRNEYKNAGY
VMTAWKNPLLNARVSLRYSILMFPLCFGLSYFNITDWYYQIDSGLINAWLTFWAFKFY
WQQRINYSAKTLKDNVKFNKGLSVANIYARKTFMASVLHLPAILILAIIHKKGRWDWI
YPGEAKRPQERF"
gene complement(<226169..>227371)
/gene="OYE3"
/locus_tag="YPL171C"
/gene_synonym="ZRG6"
/db_xref="GeneID:855932"
mRNA complement(<226169..>227371)
/gene="OYE3"
/locus_tag="YPL171C"
/gene_synonym="ZRG6"
/product="NADPH dehydrogenase"
/transcript_id="NM_001183985.1"
/db_xref="GeneID:855932"
CDS complement(226169..227371)
/gene="OYE3"
/locus_tag="YPL171C"
/gene_synonym="ZRG6"
/EC_number="1.6.99.1"
/experiment="EXISTENCE:direct assay:GO:0003959 NADPH
dehydrogenase activity [PMID:7836424]"
/experiment="EXISTENCE:mutant phenotype:GO:0006915
apoptotic process [PMID:17897954]"
/note="Conserved NADPH oxidoreductase containing flavin
mononucleotide (FMN); homologous to Oye2p with different
ligand binding and catalytic properties; has potential
roles in oxidative stress response and programmed cell
death"
/codon_start=1
/product="NADPH dehydrogenase"
/protein_id="NP_015154.1"
/db_xref="GeneID:855932"
/db_xref="SGD:S000006092"
/translation="MPFVKGFEPISLRDTNLFEPIKIGNTQLAHRAVMPPLTRMRATH
PGNIPNKEWAAVYYGQRAQRPGTMIITEGTFISPQAGGYDNAPGIWSDEQVAEWKNIF
LAIHDCQSFAWVQLWSLGWASFPDVLARDGLRYDCASDRVYMNATLQEKAKDANNLEH
SLTKDDIKQYIKDYIHAAKNSIAAGADGVEIHSANGYLLNQFLDPHSNKRTDEYGGTI
ENRARFTLEVVDALIETIGPERVGLRLSPYGTFNSMSGGAEPGIIAQYSYVLGELEKR
AKAGKRLAFVHLVEPRVTDPSLVEGEGEYSEGTNDFAYSIWKGPIIRAGNYALHPEVV
REQVKDPRTLIGYGRFFISNPDLVYRLEEGLPLNKYDRSTFYTMSAEGYTDYPTYEEA
VDLGWNKN"
gene <228314..>228772
/gene="DAP1"
/locus_tag="YPL170W"
/db_xref="GeneID:855933"
mRNA <228314..>228772
/gene="DAP1"
/locus_tag="YPL170W"
/product="Dap1p"
/transcript_id="NM_001183984.1"
/db_xref="GeneID:855933"
CDS 228314..228772
/gene="DAP1"
/locus_tag="YPL170W"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:17954932]"
/experiment="EXISTENCE:direct assay:GO:0020037 heme
binding [PMID:15713626]"
/experiment="EXISTENCE:genetic interaction:GO:0032443
regulation of ergosterol biosynthetic process
[PMID:15713626]"
/experiment="EXISTENCE:mutant phenotype:GO:0032443
regulation of ergosterol biosynthetic process
[PMID:17954932]"
/note="Heme-binding protein; involved in regulation of
cytochrome P450 protein Erg11p; damage response protein,
related to mammalian membrane progesterone receptors;
mutations lead to defects in telomeres, mitochondria, and
sterol synthesis"
/codon_start=1
/product="Dap1p"
/protein_id="NP_015155.1"
/db_xref="GeneID:855933"
/db_xref="SGD:S000006091"
/translation="MSFIKNLLFGGVKTSEDPTGLTGNGASNTNDSNKGSEPVVAGNF
FPRTLSKFNGHDDEKIFIAIRGKVYDCTRGRQFYGPSGPYTNFAGHDASRGLALNSFD
LDVIKDWDQPIDPLDDLTKEQIDALDEWQEHFENKYPCIGTLIPEPGVNV"
gene complement(<229039..>230838)
/gene="MEX67"
/locus_tag="YPL169C"
/db_xref="GeneID:855934"
mRNA complement(<229039..>230838)
/gene="MEX67"
/locus_tag="YPL169C"
/product="Mex67p"
/transcript_id="NM_001183983.1"
/db_xref="GeneID:855934"
CDS complement(229039..230838)
/gene="MEX67"
/locus_tag="YPL169C"
/experiment="EXISTENCE:direct assay:GO:0000049 tRNA
binding [PMID:29212662]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:12524544]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10684247]"
/experiment="EXISTENCE:direct assay:GO:0005643 nuclear
pore [PMID:31753862]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:10684247]"
/experiment="EXISTENCE:direct assay:GO:0015288 porin
activity [PMID:31753862]"
/experiment="EXISTENCE:direct assay:GO:0017070 U6 snRNA
binding [PMID:31189105]"
/experiment="EXISTENCE:direct assay:GO:0030619 U1 snRNA
binding [PMID:31189105]"
/experiment="EXISTENCE:direct assay:GO:0030620 U2 snRNA
binding [PMID:31189105]"
/experiment="EXISTENCE:direct assay:GO:0030621 U4 snRNA
binding [PMID:31189105]"
/experiment="EXISTENCE:direct assay:GO:0030623 U5 snRNA
binding [PMID:31189105]"
/experiment="EXISTENCE:genetic interaction:GO:0000055
ribosomal large subunit export from nucleus
[PMID:17434126]"
/experiment="EXISTENCE:mutant phenotype:GO:0000055
ribosomal large subunit export from nucleus
[PMID:17434126|PMID:31439809]"
/experiment="EXISTENCE:mutant phenotype:GO:0000056
ribosomal small subunit export from nucleus
[PMID:22956913]"
/experiment="EXISTENCE:mutant phenotype:GO:0006406 mRNA
export from nucleus [PMID:10722667]"
/experiment="EXISTENCE:mutant phenotype:GO:0006409 tRNA
export from nucleus [PMID:29212662]"
/experiment="EXISTENCE:mutant phenotype:GO:0008033 tRNA
processing [PMID:29212662]"
/experiment="EXISTENCE:mutant phenotype:GO:0016973
poly(A)+ mRNA export from nucleus [PMID:27385342]"
/experiment="EXISTENCE:mutant phenotype:GO:0071528 tRNA
re-export from nucleus [PMID:33074312]"
/experiment="EXISTENCE:physical interaction:GO:0042272
nuclear RNA export factor complex [PMID:9774696]"
/note="Poly(A) RNA binding protein involved in nuclear
mRNA export; component of the nuclear pore and nuclear RNA
export factor; ubiquitin-associated (UBA) domain interacts
with Mip6p; ortholog of human TAP"
/codon_start=1
/product="Mex67p"
/protein_id="NP_015156.1"
/db_xref="GeneID:855934"
/db_xref="SGD:S000006090"
/translation="MSGFHNVGNINMMAQQQMQQNRIKISVRNWQNATMNDLINFISR
NARVAVYDAHVEGPLVIGYVNSKAEAESLMKWNGVRFAGSNLKFELLDNNGASAGTSD
TISFLRGVLLKRYDPQTKLLNLGALHSDPELIQKGVFSSISTQSKMFPAMMKLASTEK
SLIVESVNLADNQLKDISAISTLAQTFPNLKNLCLANNQIFRFRSLEVWKNKFKDLRE
LLMTNNPITTDKLYRTEMLRLFPKLVVLDNVIVRDEQKLQTVYSLPMKIQQFFFENDA
LGQSSTDFATNFLNLWDNNREQLLNLYSPQSQFSVSVDSTIPPSTVTDSDQTPAFGYY
MSSSRNISKVSSEKSIQQRLSIGQESINSIFKTLPKTKHHLQEQPNEYSMETISYPQI
NGFVITLHGFFEETGKPELESNKKTGKNNYQKNRRYNHGYNSTSNNKLSKKSFDRTWV
IVPMNNSVIIASDLLTVRAYSTGAWKTASIAIAQPPQQQASVLPQVASMNPNITTPPQ
PQPSVVPGGMSIPGAPQGAMVMAPTLQLPPDVQSRLNPVQLELLNKLHLETKLNAEYT
FMLAEQSNWNYEVAIKGFQSSMNGIPREAFVQF"
gene <231217..>232509
/gene="MRX4"
/locus_tag="YPL168W"
/db_xref="GeneID:855935"
mRNA <231217..>232509
/gene="MRX4"
/locus_tag="YPL168W"
/product="Mrx4p"
/transcript_id="NM_001183982.1"
/db_xref="GeneID:855935"
CDS 231217..232509
/gene="MRX4"
/locus_tag="YPL168W"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:40637229]"
/experiment="EXISTENCE:mutant phenotype:GO:0006446
regulation of translational initiation [PMID:40637229]"
/experiment="EXISTENCE:mutant phenotype:GO:0045182
translation regulator activity [PMID:40637229]"
/note="Translation regulator of mitochondrial matrix;
ligand of the mitoribosomal polypeptide tunnel exit;
mediates the feedback loop necessary for repression of COB
translation"
/codon_start=1
/product="Mrx4p"
/protein_id="NP_015157.1"
/db_xref="GeneID:855935"
/db_xref="SGD:S000006089"
/translation="MTVLYTSASLKKMKCLAFNMGMNCVRTVSHARSGGAKFGGRNVF
NIFDSKTPDSVRIKAFKNTIYQSAMGKGKTKFSAMEINLITSLVRGYKGEGKKNAINP
LQTNVQILNKLLLTHRLTDKDILEGMNLAAGPVNVAIPRDITPQEEKKKVELRNRKAE
NMDLHPSRKMHIKELLHSLNLDMCNDEEVYQKISLYLQKNEESRTSVGASQQNHVDID
INSLKRYLQNIEKKARQKSAIDKQKKNQARIYQWNTQSFSEIVPLSAGNILFKREPNR
LWKRLQNGISVFLGSNGGGKKSKTTKKVLQGNNILLHSLENNKDMTLSNNFDHSVFNI
NFTDLFGVINASGSPPDRVLNEINEIELKGWKCVGNLYDNNKIVVFQSSNPLLEDTKI
PQKSFTNSKRFLISLSALLASFFAYYRYRLSQRQESKK"
gene complement(<232593..>237107)
/gene="REV3"
/locus_tag="YPL167C"
/gene_synonym="PSO1"
/db_xref="GeneID:855936"
mRNA complement(<232593..>237107)
/gene="REV3"
/locus_tag="YPL167C"
/gene_synonym="PSO1"
/product="DNA-directed DNA polymerase"
/transcript_id="NM_001183981.1"
/db_xref="GeneID:855936"
CDS complement(232593..237107)
/gene="REV3"
/locus_tag="YPL167C"
/gene_synonym="PSO1"
/EC_number="2.7.7.7"
/experiment="EXISTENCE:direct assay:GO:0003887
DNA-directed DNA polymerase activity [PMID:8658138]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16452144]"
/experiment="EXISTENCE:direct assay:GO:0016035 zeta DNA
polymerase complex
[PMID:23066099|PMID:22711820|PMID:8658138]"
/experiment="EXISTENCE:direct assay:GO:0042276 error-prone
translesion synthesis [PMID:17248528|PMID:11313481]"
/experiment="EXISTENCE:direct assay:GO:0070987 error-free
translesion synthesis [PMID:12514101]"
/experiment="EXISTENCE:genetic interaction:GO:0042276
error-prone translesion synthesis [PMID:9765213]"
/note="Catalytic subunit of DNA polymerase zeta (pol
zeta); involved in translesion synthesis during
post-replication repair; required for mutagenesis induced
by DNA damage; involved in double-strand break repair; may
be involved in meiosis; forms a complex with Rev7p, Pol31p
and Pol32p"
/codon_start=1
/product="DNA-directed DNA polymerase"
/protein_id="NP_015158.1"
/db_xref="GeneID:855936"
/db_xref="SGD:S000006088"
/translation="MSRESNDTIQSDTVRSSSKSDYFRIQLNNQDYYMSKPTFLDPSH
GESLPLNQFSQVPNIRVFGALPTGHQVLCHVHGILPYMFIKYDGQITDTSTLRHQRCA
QVHKTLEVKIRASFKRKKDDKHDLAGDKLGNLNFVADVSVVKGIPFYGYHVGWNLFYK
ISLLNPSCLSRISELIRDGKIFGKKFEIYESHIPYLLQWTADFNLFGCSWINVDRCYF
RSPVLNSILDIDKLTINDDLQLLLDRFCDFKCNVLSRRDFPRVGNGLIEIDILPQFIK
NREKLQHRDIHHDFLEKLGDISDIPVKPYVSSARDMINELTMQREELSLKEYKEPPET
KRHVSGHQWQSSGEFEAFYKKAQHKTSTFDGQIPNFENFIDKNQKFSAINTPYEALPQ
LWPRLPQIEINNNSMQDKKNDDQVNASFTEYEICGVDNENEGVKGSNIKSRSYSWLPE
SIASPKDSTILLDHQTKYHNTINFSMDCAMTQNMASKRKLRSSVSANKTSLLSRKRKK
VMAAGLRYGKRAFVYGEPPFGYQDILNKLEDEGFPKIDYKDPFFSNPVDLENKPYAYA
GKRFEISSTHVSTRIPVQFGGETVSVYNKPTFDMFSSWKYALKPPTYDAVQKWYNKVP
SMGNKKTESQISMHTPHSKFLYKFASDVSGKQKRKKSSVHDSLTHLTLEIHANTRSDK
IPDPAIDEVSMIIWCLEEETFPLDLDIAYEGIMIVHKASEDSTFPTKIQHCINEIPVM
FYESEFEMFEALTDLVLLLDPDILSGFEIHNFSWGYIIERCQKIHQFDIVRELARVKC
QIKTKLSDTWGYAHSSGIMITGRHMINIWRALRSDVNLTQYTIESAAFNILHKRLPHF
SFESLTNMWNAKKSTTELKTVLNYWLSRAQINIQLLRKQDYIARNIEQARLIGIDFHS
VYYRGSQFKVESFLIRICKSESFILLSPGKKDVRKQKALECVPLVMEPESAFYKSPLI
VLDFQSLYPSIMIGYNYCYSTMIGRVREINLTENNLGVSKFSLPRNILALLKNDVTIA
PNGVVYAKTSVRKSTLSKMLTDILDVRVMIKKTMNEIGDDNTTLKRLLNNKQLALKLL
ANVTYGYTSASFSGRMPCSDLADSIVQTGRETLEKAIDIIEKDETWNAKVVYGDTDSL
FVYLPGKTAIEAFSIGHAMAERVTQNNPKPIFLKFEKVYHPSILISKKRYVGFSYESP
SQTLPIFDAKGIETVRRDGIPAQQKIIEKCIRLLFQTKDLSKIKKYLQNEFFKIQIGK
VSAQDFCFAKEVKLGAYKSEKTAPAGAVVVKRRINEDHRAEPQYKERIPYLVVKGKQG
QLLRERCVSPEEFLEGENLELDSEYYINKILIPPLDRLFNLIGINVGNWAQEIVKSKR
ASTTTTKVENITRVGTSATCCNCGEELTKICSLQLCDDCLEKRSTTTLSFLIKKLKRQ
KEYQTLKTVCRTCSYRYTSDAGIENDHIASKCNSYDCPVFYSRVKAERYLRDNQSVQR
EEALISLNDW"
gene <237338..>237979
/gene="ATG29"
/locus_tag="YPL166W"
/db_xref="GeneID:855937"
mRNA <237338..>237979
/gene="ATG29"
/locus_tag="YPL166W"
/product="Atg29p"
/transcript_id="NM_001183980.1"
/db_xref="GeneID:855937"
CDS 237338..237979
/gene="ATG29"
/locus_tag="YPL166W"
/experiment="EXISTENCE:direct assay:GO:0000045
autophagosome assembly [PMID:18725539]"
/experiment="EXISTENCE:direct assay:GO:0000149 SNARE
binding [PMID:26774783]"
/experiment="EXISTENCE:direct assay:GO:0000407 phagophore
assembly site [PMID:16289106]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0006995 cellular
response to nitrogen starvation
[PMID:18725539|PMID:26774783]"
/experiment="EXISTENCE:direct assay:GO:0034497 protein
localization to phagophore assembly site [PMID:26774783]"
/experiment="EXISTENCE:direct assay:GO:1990316 Atg1/ULK1
kinase complex [PMID:26753620]"
/experiment="EXISTENCE:mutant phenotype:GO:0000422
autophagy of mitochondrion [PMID:19793921]"
/experiment="EXISTENCE:mutant phenotype:GO:0016236
macroautophagy [PMID:16289106]"
/experiment="EXISTENCE:mutant phenotype:GO:0034727
piecemeal microautophagy of the nucleus [PMID:18701704]"
/experiment="EXISTENCE:mutant phenotype:GO:0044804
nucleophagy [PMID:22768199]"
/note="Autophagy-specific protein; required for recruiting
other ATG proteins to the pre-autophagosomal structure
(PAS); interacts with Atg17p and localizas to the PAS in a
manner interdependent with Atg17p and Cis1p; not
conserved; relocalizes from nucleus to cytoplasmic foci
upon DNA replication stress"
/codon_start=1
/product="Atg29p"
/protein_id="NP_015159.1"
/db_xref="GeneID:855937"
/db_xref="SGD:S000006087"
/translation="MIMNSTNTVVYIKVKGRRPQGFLDPPKFEWNGTKERQLWTMVSN
LNYSQDQIDWQNLSKIFETPEFFLKKRTYKLFAEHLELLQLQLEKKRDLEKYSNDQVN
EGMSDLIHKYTPTLQNDNLLNVSASPLTTERQDSEEVETEVTNEALQHLQTSKILNIH
KKTSDSENKPNDKLDKDGINKEMECGSSDDDLSSSLSVSKSALEEALMDRLQF"
gene complement(<237956..>239077)
/gene="SET6"
/locus_tag="YPL165C"
/db_xref="GeneID:855938"
mRNA complement(<237956..>239077)
/gene="SET6"
/locus_tag="YPL165C"
/product="Set6p"
/transcript_id="NM_001183979.1"
/db_xref="GeneID:855938"
CDS complement(237956..239077)
/gene="SET6"
/locus_tag="YPL165C"
/note="SET domain hypothetical protein; deletion
heterozygote is sensitive to compounds that target
ergosterol biosynthesis, may be involved in compound
availability"
/codon_start=1
/product="Set6p"
/protein_id="NP_015160.1"
/db_xref="GeneID:855938"
/db_xref="SGD:S000006086"
/translation="MTIDGDVHEISPFFQVRQTKWGGRACFSNGNIPKGTTVLQVSNF
TGTSISYEFRKEVCHNCFAYANAKTMKYKLNYDYLRDLVCNAHYQINPKKFLGAGLWF
CSEHCRTSYLQIPNIIELIECYEILLHHFPSMLKRYNYTSEQEEKLNSILISENVIQS
SWDEIESKWIPRINNMKSAKRINQLPPTCEDEYCCIRFVCESLFNLKYMDPQCITYRA
FNMLQSNELSKISKFPVLLHFQKLVFQTLYILLPSHLHRMLSIPLLRHILGTEYGNAF
GLWQEGEASDSREYFGYWVFPEASYFNHSCNPNITKYRKGNSMLFTMNRDIKKDEQIC
IDYSGVLDLPTVKRRAFLADSWFFDCACERCKSELQSVH"
gene complement(<239350..>241497)
/gene="MLH3"
/locus_tag="YPL164C"
/db_xref="GeneID:855939"
mRNA complement(<239350..>241497)
/gene="MLH3"
/locus_tag="YPL164C"
/product="mismatch repair protein MLH3"
/transcript_id="NM_001183978.1"
/db_xref="GeneID:855939"
CDS complement(239350..241497)
/gene="MLH3"
/locus_tag="YPL164C"
/experiment="EXISTENCE:mutant phenotype:GO:0000710 meiotic
mismatch repair [PMID:23316435]"
/experiment="EXISTENCE:mutant phenotype:GO:0006298
mismatch repair [PMID:10679328|PMID:9770499]"
/experiment="EXISTENCE:mutant phenotype:GO:0007131
reciprocal meiotic recombination [PMID:10570173]"
/experiment="EXISTENCE:physical interaction:GO:0005634
nucleus [PMID:10570173]"
/experiment="EXISTENCE:physical interaction:GO:0097587
MutLgamma complex [PMID:10570173|PMID:9770499]"
/note="Protein involved in DNA mismatch repair and meiotic
recombination; involved in crossing-over during meiotic
recombination; forms a complex with Mlh1p; mammalian
homolog is implicated mammalian microsatellite
instability"
/codon_start=1
/product="mismatch repair protein MLH3"
/protein_id="NP_015161.1"
/db_xref="GeneID:855939"
/db_xref="SGD:S000006085"
/translation="MSQHIRKLDSDVSERLKSQACTVSLASAVREIVQNSVDAHATTI
DVMIDLPNLSFAVYDDGIGLTRSDLNILATQNYTSKIRKMNDLVTMKTYGYRGDALYS
ISNVSNLFVCSKKKDYNSAWMRKFPSKSVMLSENTILPIDPFWKICPWSRTKSGTVVI
VEDMLYNLPVRRRILKEEPPFKTFNTIKADMLQILVMHPMISLNVQYTDKLRINTEVL
FRSKNITEGLTKHQQMSQVLRNVFGAIIPPDMLKKVSLKFNEYQIEGIISKMPVGLKD
LQFIYINGRRYADSAFQGYVDSLFQAQDFGEKGMSLLKTKSVGKPYRSHPVFILDVRC
PQTIDDLLQDPAKKIVKPSHIRTIEPLIVKTIRSFLTFQGYLTPDKSDSSFEIVNCSQ
KTATLPDSRIQISKRNQVLNSKMKIARINSYIGKPAVNGCRINNSTINYEKIKNIRID
GQKSRLRNKLSSRPYDSGFTEDYDSIGKTITDFSISRSVLAKYEVINQVDKKFILIRC
LDQSIHNCPLLVLVDQHACDERIRLEELFYSLLTEVVTGTFVARDLKDCCIEVDRTEA
DLFKHYQSEFKKWGIGYETIEGTMETSLLEIKTLPEMLTSKYNGDKDYLKMVLLQHAH
DLKDFKKLPMDLSHFENYTSVDKLYWWKYSSCVPTVFHEILNSKACRSAVMFGDELTR
QECIILISKLSRCHNPFECAHGRPSMVPIAELK"
gene complement(<241918..>242700)
/gene="SVS1"
/locus_tag="YPL163C"
/db_xref="GeneID:855940"
mRNA complement(<241918..>242700)
/gene="SVS1"
/locus_tag="YPL163C"
/product="Svs1p"
/transcript_id="NM_001183977.1"
/db_xref="GeneID:855940"
CDS complement(241918..242700)
/gene="SVS1"
/locus_tag="YPL163C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:11935221]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="Cell wall and vacuolar protein; required for
wild-type resistance to vanadate; SVS1 has a paralog,
SRL1, that arose from the whole genome duplication"
/codon_start=1
/product="Svs1p"
/protein_id="NP_015162.1"
/db_xref="GeneID:855940"
/db_xref="SGD:S000006084"
/translation="MIFKILCSLLLVTSNFASALYVNETTSYTPYTKTLTPTYSVSPQ
ETTLTYSDETTTFYITSTFYSTYWFTTSQSAAIISTPTASTPTASTPSLTTSTNEYTT
TYSDTDTTYTSTLTSTYIITLSTESANEKAEQISTSVTEIASTVTESGSTYTSTLTST
LLVTVYNSQASNTIATSTAGDAASNVDALEKLVSAEHQSQMIQTTSADEQYCSASTKY
VTVTAAAVTEVVTTTAEPVVKYVTITADASNVTGSANNGTHI"
gene complement(<243206..>244027)
/locus_tag="YPL162C"
/db_xref="GeneID:855941"
mRNA complement(<243206..>244027)
/locus_tag="YPL162C"
/product="uncharacterized protein"
/transcript_id="NM_001183976.1"
/db_xref="GeneID:855941"
CDS complement(243206..244027)
/locus_tag="YPL162C"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:14562095]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to the membrane of vacuole
with cell cycle-correlated morphology"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015163.1"
/db_xref="GeneID:855941"
/db_xref="SGD:S000006083"
/translation="MYVSNGKDTCQLLGPVSLFVQTLMGMTAVIVLLVKRNYEHPRRK
MIVWSYDIGKQIIGSLGIHFLNLGISILKKRRRSLFAITAKGNDDEDQCDWYFLNLLL
DTTVGIPILWLCLYIIEKVLKSLHFQNIESGNYFPSKTVGSHPRKPLFSAFVKQLLIF
IVGLGVMKFCVFLILNYLEDLAYWFADLILGWSDSWPNFQVFLVMFVFPILLNCFQYF
CVDNVIRLHSESLTITNAENFETNTFLNDEIPDLSEVSNEVPNKDNNISSYGSII"
gene complement(<244319..>246220)
/gene="BEM4"
/locus_tag="YPL161C"
/gene_synonym="ROM7"
/db_xref="GeneID:855942"
mRNA complement(<244319..>246220)
/gene="BEM4"
/locus_tag="YPL161C"
/gene_synonym="ROM7"
/product="Bem4p"
/transcript_id="NM_001183975.1"
/db_xref="GeneID:855942"
CDS complement(244319..246220)
/gene="BEM4"
/locus_tag="YPL161C"
/gene_synonym="ROM7"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0007266 Rho
protein signal transduction [PMID:8754839]"
/experiment="EXISTENCE:mutant phenotype:GO:0030010
establishment of cell polarity [PMID:8754839]"
/note="Protein involved in establishment of cell polarity
and bud emergence; regulates filamentous-growth
mitogen-activated protein kinase (MAPK) pathway; interacts
with the Rho1p small GTP-binding protein, the pleckstrin
homology (PH) domain of Cdc24p, and with the Rho-type
GTPase Cdc42p; stabilizes Cdc42p levels, resulting in
elevated fMAPK pathway signaling; involved in maintenance
of proper telomere length"
/codon_start=1
/product="Bem4p"
/protein_id="NP_015164.1"
/db_xref="GeneID:855942"
/db_xref="SGD:S000006082"
/translation="MDYEEILFGLQPILNASSIKDVPMNDVYLGSYLAVMDQLAVSLR
EPSNRDIVGKTGLLLNLVRVLEQALDICFHDTSISINDKIAFYEISSEVIRCIANAII
DNDDNREILLDSGGKKLLNYYIGGVLQLDEISSDKSEDSLVDKLQMRSVVLLRNFCIG
NLKYTENLAPFIRGPLFVLLKTTQYSYLSSPEKVVLGSDLLNDILKVNYSNVQISDLF
FLSQYIKKISSNVQNKELQAMEDGAVEAYSNTETQKFAGQGNQEYIEKEEEDDEEDVN
CELLLNLSTCLETIVAKDETINFTNEEQLVLSMQKNLILSLVCLESKTFNNKLIVMRR
LISCAGNISANLTNSNKREQSLCIETIKSSASSYALAAALMILCNSVASKSDAVALLK
LISLSELIQVGSLLQDPLQYQGFLDLLRKLLNLENTMWLDIKDLFTLFQIMRRCHEQT
KYYNNLRSLLTNLLNKTLTVLPSSKIHNSISSDPTIISFIAEHGTLTSCIAMDKLLVS
KKALPKEAITSLWDSIFKFQNLGQAEQLSISDLFHITKTVGIYLKDSSVTADVNPIEN
ILFKDYIQKLTLILETILSFKENKDKGSESCFNNGKFIAGIILNIVKNTKCLTPEEQN
LEALAKSFF"
gene <246990..>250262
/gene="CDC60"
/locus_tag="YPL160W"
/db_xref="GeneID:855943"
mRNA <246990..>250262
/gene="CDC60"
/locus_tag="YPL160W"
/product="leucine--tRNA ligase CDC60"
/transcript_id="NM_001183974.1"
/db_xref="GeneID:855943"
CDS 246990..250262
/gene="CDC60"
/locus_tag="YPL160W"
/EC_number="6.1.1.4"
/experiment="EXISTENCE:direct assay:GO:0004823
leucine-tRNA ligase activity [PMID:9742237]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:9742237]"
/experiment="EXISTENCE:direct assay:GO:0006429 leucyl-tRNA
aminoacylation [PMID:9742237]"
/experiment="EXISTENCE:direct assay:GO:1990825
sequence-specific mRNA binding [PMID:34039240]"
/experiment="EXISTENCE:mutant phenotype:GO:1903432
regulation of TORC1 signaling [PMID:22424774]"
/experiment="EXISTENCE:physical interaction:GO:1903432
regulation of TORC1 signaling [PMID:22424774]"
/note="Cytosolic leucyl tRNA synthetase; ligates leucine
to the appropriate tRNA; human homolog LARS can complement
yeast temperature-sensitive mutant at restrictive
temperature"
/codon_start=1
/product="leucine--tRNA ligase CDC60"
/protein_id="NP_015165.1"
/db_xref="GeneID:855943"
/db_xref="SGD:S000006081"
/translation="MSSGLVLENTARRDALIAIEKKYQKIWAEEHQFEIDAPSIEDEP
ITMDSEELHRTYPKFMSSMAYPYMNGVMHAGHCFTLSKVEFSIGFERMNGKRALFPLG
FHCTGMPILACADKLKREAELFGKNFDNVPAEEEEIKEETPAEKDHEDVTKFKAKKSK
AAAKKGRGKYQFEIMLQLGIPREEIIKFADAKYWLTYFPPLCESDCTSLGARIDWRRS
FVTTDANPYYDAFIRWQMNKLKAAGKIKFGERYTIYSEKDGQACMDHDRQSGEGVTPQ
EYIGVKIEALEFADDAAKIIDSSSDLDKSKKFYFVAATLRPETMYGQTCCFVSPTIEY
GIFDAGDSYFITTERAFKNMSYQKLTPKRGFYKPIVTVPGKAFIGTKIHAPQSVYPEL
RILPMETVIATKGTGVVTCVPSNSPDDYITTKDLLHKPEYYGIKPEWIDHEIVPIMHT
EKYGDLTAKAIVEEKKIQSPKDKNLLAEAKKIAYKEDYYTGTMIYGPYKGEKVEQAKN
KVKADMIAAGEAFVYNEPESQVMSRSGDDCIVSLEDQWYVDYGEESWKKQAIECLEGM
QLFAPEVKNAFEGVLDWLKNWAVCRTYGLGTRLPWDEKYLVESLSDSTIYQSFYTIAH
LLFKDYYGNEIGPLGISADQMTDEVFDYIFQHQDDVKNTNIPLPALQKLRREFEYFYP
LDVSISGKDLIPNHLTFFIYTHVALFPKKFWPKGIRANGHLMLNNSKMSKSTGNFMTL
EQTVEKFGADAARIAFADAGDTVEDANFDESNANAAILRLFNLKEWAEEITKESNLRT
GEITDFFDIAFEHEMNALIEKTYEQYALTNYKNALKYGLFDFQAARDYYREASGVMHK
DLIARYIETQALLLAPIAPHFAEYIYREVLGNQTSVQNAKFPRASKPVDKGVLAALDY
LRNLQRSIREGEGQALKKKKGKSAEIDASKPVKLTLLISESFPEWQSQCVEIVRKLFS
EQTLDDNKKVREHIEPKEMKRAMPFISLLKQRLANEKPEDVFERELQFSEIDTVKAAA
RNVKKAAQALKIAEFSAISFPYGAKTGKDIFTGEEVEIPPVTKIVENAVPGNPGVVFQ
NI"
gene complement(<250907..>251668)
/gene="PET20"
/locus_tag="YPL159C"
/db_xref="GeneID:855944"
mRNA complement(<250907..>251668)
/gene="PET20"
/locus_tag="YPL159C"
/product="Pet20p"
/transcript_id="NM_001183973.1"
/db_xref="GeneID:855944"
CDS complement(250907..251668)
/gene="PET20"
/locus_tag="YPL159C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16491469]"
/experiment="EXISTENCE:mutant phenotype:GO:0009060 aerobic
respiration [PMID:16491469]"
/note="Mitochondrial protein; required for respiratory
growth under some conditions and for stability of the
mitochondrial genome; subunit of a complex containing
Mrx6p, Pim1p, and Mam33p that may regulate mtDNA
replication"
/codon_start=1
/product="Pet20p"
/protein_id="NP_015166.1"
/db_xref="GeneID:855944"
/db_xref="SGD:S000006080"
/translation="MLKLARPFIPPLSRNNAISSGIVLTSRRFQSSFTFLSNQSLLSK
NQMKSKRKKGSKKAAYHRQPPEHEHTAPLIKQNKTITKKEHSDVRGSHLKKKRSDFSW
LPRVPSTSHLKQSDMTTNVLYSGYRPLFINPNDPKLKEDTGSTLYEFAMKLEDLNEPL
SPWISSATGLEFFSEWENIPSELLKNLKPFHPPKEKSMNTNELIHVSAKRNTLVDNKT
SETLQRKMDEFSKRRGKGRKKSVVTLLQMKKKLEG"
gene complement(<252034..>254310)
/gene="AIM44"
/locus_tag="YPL158C"
/gene_synonym="GPS1"
/db_xref="GeneID:855945"
mRNA complement(<252034..>254310)
/gene="AIM44"
/locus_tag="YPL158C"
/gene_synonym="GPS1"
/product="Aim44p"
/transcript_id="NM_001183972.1"
/db_xref="GeneID:855945"
CDS complement(252034..254310)
/gene="AIM44"
/locus_tag="YPL158C"
/gene_synonym="GPS1"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:28148650]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck
[PMID:14562095|PMID:28148650|PMID:22842922|PMID:23468594|P
MID:24451263]"
/experiment="EXISTENCE:direct assay:GO:0032153 cell
division site [PMID:25416945]"
/experiment="EXISTENCE:direct assay:GO:0032174 cellular
bud neck septin collar [PMID:30341817]"
/experiment="EXISTENCE:direct assay:GO:0032177 cellular
bud neck split septin rings [PMID:30341817]"
/experiment="EXISTENCE:genetic interaction:GO:1990344
secondary cell septum biogenesis [PMID:23468594]"
/experiment="EXISTENCE:mutant phenotype:GO:0005637 nuclear
inner membrane [PMID:30341817]"
/experiment="EXISTENCE:mutant phenotype:GO:0005886 plasma
membrane [PMID:30341817]"
/experiment="EXISTENCE:mutant phenotype:GO:0005935
cellular bud neck [PMID:23468594]"
/experiment="EXISTENCE:mutant phenotype:GO:0045185
maintenance of protein location [PMID:23468594]"
/experiment="EXISTENCE:mutant phenotype:GO:0098841 protein
localization to cell division site after cytokinesis
[PMID:25416945]"
/experiment="EXISTENCE:mutant phenotype:GO:1901900
regulation of protein localization to cell division site
[PMID:23468594]"
/experiment="EXISTENCE:mutant phenotype:GO:1903473
positive regulation of mitotic actomyosin contractile ring
contraction [PMID:24451263]"
/experiment="EXISTENCE:mutant phenotype:GO:1990344
secondary cell septum biogenesis [PMID:23468594]"
/note="Regulator of Cdc42p and Rho1p; regulates AMR
closure through Hof1p; inhibits Cdc42-dependent Cla4
activation at the division site, to prevent budding in the
old bud neck; recruits Nis1p and Nba1p to the division
site with Nap1 and the Rax1p-Rax2p dependent inheritance
of Nis1p and Nba1p to bud scars to prevent division site
repolarization; keeps Rho1p at the division site after AMR
contraction to control secondary septum formation;
relocalizes from bud neck to cytoplasm upon replication
stress"
/codon_start=1
/product="Aim44p"
/protein_id="NP_015167.1"
/db_xref="GeneID:855945"
/db_xref="SGD:S000006079"
/translation="MIIRAPIRTKTKSFRGDQMDFKFPSNESLPRGTLEEYHLNNHHL
LNDVFAAENGVSRDEDGNSQTLSDYTSTSNTNTNSGYSSNGYYSFANISDNTTSSPRI
VINQNETARLTSSDSNKSDFFASHDFPGNDSLHYSSSNVVKNQLHSMEAIPEGNITGS
ISTAFQTIPTADNVSYDIAPSSASSLLPRKSTSKSAILPSTQEAKPMTKLNMEKDIKT
IELNNSVVPKPKKKLNRVPTIRRVESSRFSNSRYSSSVSSKSSSSRCSLKRSKAIRCK
GGLLYYFTSLGIKIKKKLRKLRLVLRRRLFSYNVQKVPSATNSKTTKSKANINNKSKK
RGTNLVNKNSNSTPRQKKSQRYVSNLQRSISSKSLVPVLAPQKKTKPLTVDTKFKANH
PQSEDSKVGSNTPRSPLVSYTPSLRRTNSSIRRAASILTASATMTPANNKNSFISVPD
NVSHAVTRNSSMYSRSRLVRSKPSTALNAIARQPSIVVENKVIPLSMNRYSIKEEDEY
VIDTSSMRELSPVNSVCSSDYDRESSESYSNYADAMETTEVDNKDRVECNNEIQNVDA
NNEETSNEESYNLMKHYLSTVIAQRIMLRVQIARIQNYKSNVVYMNKSAETNSTIYED
LVDSLLTEYEADGSSSQIFDGVTVRADEEEEEDEDDEDDEEEEEENDDEEDEEDEEDD
EDDEEEEEKRKEGEGRNLAKEVDELAELSPMRKQSDLSITLRSPFAMLNPAYSNSIIS
LPTGVVKRSLTLPVGMKI"
gene <254814..>255761
/gene="TGS1"
/locus_tag="YPL157W"
/db_xref="GeneID:855946"
mRNA <254814..>255761
/gene="TGS1"
/locus_tag="YPL157W"
/product="RNA methyltransferase"
/transcript_id="NM_001183971.1"
/db_xref="GeneID:855946"
CDS 254814..255761
/gene="TGS1"
/locus_tag="YPL157W"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:11983179]"
/experiment="EXISTENCE:mutant phenotype:GO:0001510 RNA
methylation [PMID:11983179]"
/experiment="EXISTENCE:mutant phenotype:GO:0008033 tRNA
processing [PMID:27348091]"
/experiment="EXISTENCE:mutant phenotype:GO:0008173 RNA
methyltransferase activity [PMID:11983179]"
/experiment="EXISTENCE:mutant phenotype:GO:0017126
nucleologenesis [PMID:15340060]"
/experiment="EXISTENCE:mutant phenotype:GO:0032210
regulation of telomere maintenance via telomerase
[PMID:18273059|PMID:18840651]"
/experiment="EXISTENCE:mutant phenotype:GO:0036261
7-methylguanosine cap hypermethylation [PMID:11983179]"
/experiment="EXISTENCE:mutant phenotype:GO:0051321 meiotic
cell cycle [PMID:21398639]"
/note="Trimethyl guanosine synthase, conserved nucleolar
methyltransferase; converts the m(7)G cap structure of
snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G;
also required for nucleolar assembly and splicing of
meiotic pre-mRNAs; interacts with Swm2p, which may confer
substrate specificity on Tgs1p"
/codon_start=1
/product="RNA methyltransferase"
/protein_id="NP_015168.1"
/db_xref="GeneID:855946"
/db_xref="SGD:S000006078"
/translation="MGRTFIHASKIKHAARKRKHHSNFRTLIKLLNNDAYKIESSKPL
KNGKLFKYWKNRRRLFSKIDSASIYMTDELWFSVTPERIACFLANFVKACMPNAERIL
DVFCGGGGNTIQFAMQFPYVYGVDYSIEHIYCTAKNAQSYGVDDRIWLKRGSWKKLVS
KQKLSKIKYDCVFGSPPWGGPEYLRNDVYDLEQHLKPMGITKMLKSFLKLSPNVIMFL
PRNSDLNQLSRATRKVLGPFAKCKVLYVKENGYMKGIFCMWGECFFNYEPASTENSRR
ESSEKEELSSENEELSKRKKHESTTTTKDNTVDIYDVNG"
gene complement(<255913..>256767)
/gene="PRM4"
/locus_tag="YPL156C"
/db_xref="GeneID:855947"
mRNA complement(<255913..>256767)
/gene="PRM4"
/locus_tag="YPL156C"
/product="pheromone-regulated protein PRM4"
/transcript_id="NM_001183970.1"
/db_xref="GeneID:855947"
CDS complement(255913..256767)
/gene="PRM4"
/locus_tag="YPL156C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/note="Pheromone-regulated protein proposed to be involved
in mating; predicted to have 1 transmembrane segment;
transcriptionally regulated by Ste12p during mating and by
Cat8p during the diauxic shift"
/codon_start=1
/product="pheromone-regulated protein PRM4"
/protein_id="NP_015169.1"
/db_xref="GeneID:855947"
/db_xref="SGD:S000006077"
/translation="MIADSSVLKKHTAIKRSTRIISLTLVLLGVFSFLLLTWNDSLEF
YNSADPSENKKNSEEESEKKFVYKLPNLLKTADSFLSNENELNFQKVKEEISNIQSEV
EVDIPEPSSKATSKFSSRSFQTDNVVTATTTTTLNPRSSSLALQKNCDHKKFDPRTDF
LDIIRTSPAVLFIKSSQADSIFLKNLLQREFEISPELATVDLEKHSHGYELEKYIKQN
KLNIDTSAALESIQSPYLFLNGISVINRGMVRDIIEPHSKGLLLPLLKSEARGNLLVE
KKDIPSNS"
gene complement(<257216..>259336)
/gene="KIP2"
/locus_tag="YPL155C"
/db_xref="GeneID:855948"
mRNA complement(<257216..>259336)
/gene="KIP2"
/locus_tag="YPL155C"
/product="Kip2p"
/transcript_id="NM_001183969.1"
/db_xref="GeneID:855948"
CDS complement(257216..259336)
/gene="KIP2"
/locus_tag="YPL155C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005816 spindle
pole body [PMID:31490122]"
/experiment="EXISTENCE:direct assay:GO:0005881 cytoplasmic
microtubule [PMID:9693366]"
/experiment="EXISTENCE:direct assay:GO:0007018
microtubule-based movement [PMID:31490122]"
/experiment="EXISTENCE:direct assay:GO:0007026 negative
regulation of microtubule depolymerization
[PMID:26576948]"
/experiment="EXISTENCE:direct assay:GO:0015631 tubulin
binding [PMID:37093124]"
/experiment="EXISTENCE:direct assay:GO:0046785 microtubule
polymerization [PMID:37093124|PMID:26576948]"
/experiment="EXISTENCE:direct assay:GO:0061863 microtubule
plus end polymerase activity
[PMID:37093124|PMID:26576948]"
/experiment="EXISTENCE:direct assay:GO:1903754 cortical
microtubule plus-end [PMID:18411245|PMID:15177030]"
/experiment="EXISTENCE:genetic interaction:GO:0008574
plus-end-directed microtubule motor activity
[PMID:15177030|PMID:18411245]"
/experiment="EXISTENCE:mutant phenotype:GO:0007026
negative regulation of microtubule depolymerization
[PMID:37093124|PMID:9693366]"
/experiment="EXISTENCE:mutant phenotype:GO:0008574
plus-end-directed microtubule motor activity
[PMID:15177030]"
/experiment="EXISTENCE:mutant phenotype:GO:0015631 tubulin
binding [PMID:37093124]"
/experiment="EXISTENCE:mutant phenotype:GO:0030473 nuclear
migration along microtubule [PMID:9693366]"
/experiment="EXISTENCE:mutant phenotype:GO:0046785
microtubule polymerization [PMID:37093124]"
/experiment="EXISTENCE:mutant phenotype:GO:0061863
microtubule plus end polymerase activity [PMID:37093124]"
/note="Kinesin-related motor protein and plus-end directed
microtubule (MT) polymerase; involved in mitotic spindle
positioning, nuclear migration, microtubule polymerization
and inhibition of MT catastrophe; stabilizes microtubules
by targeting Bik1p to the MT plus end; Kip2p levels are
controlled during the cell cycle"
/codon_start=1
/product="Kip2p"
/protein_id="NP_015170.1"
/db_xref="GeneID:855948"
/db_xref="SGD:S000006076"
/translation="MIQKMSPSLRRPSTRSSSGSSNIPQSPSVRSTSSFSNLTRNSIR
STSNSGSQSISASSTRSNSPLRSVSAKSDPFLHPGRIRIRRSDSINNNSRKNDTYTGS
ITVTIRPKPRSVGTSRDHVGLKSPRYSQPRSNSHHGSNTFVRDPWFITNDKTIVHEEI
GEFKFDHVFASHCTNLEVYERTSKPMIDKLLMGFNATIFAYGMTGSGKTFTMSGNEQE
LGLIPLSVSYLFTNIMEQSMNGDKKFDVIISYLEIYNERIYDLLESGLEESGSRISTP
SRLYMSKSNSNGLGVELKIRDDSQYGVKVIGLTERRCESSEELLRWIAVGDKSRKIGE
TDYNARSSRSHAIVLIRLTSTNVKNGTSRSSTLSLCDLAGSERATGQQERRKEGSFIN
KSLLALGTVISKLSADKMNSVGSNIPSPSASGSSSSSGNATNNGTSPSNHIPYRDSKL
TRLLQPALSGDSIVTTICTVDTRNDAAAETMNTLRFASRAKNVALHVSKKSIISNGNN
DGDKDRTIELLRRQLEEQRRMISELKNRSNIGEPLTKSSNESTYKDIKATGNDGDPNL
ALMRAENRVLKYKLENCEKLLDKDVVDLQDSEIMEIVEMLPFEVGTLLETKFQGLESQ
IRQYRKYTQKLEDKIMALEKSGHTAMSLTGCDGTEVIELQKMLERKDKMIEALQSAKR
LRDRALKPLINTQQSPHPVVDNDK"
gene complement(<259714..>260931)
/gene="PEP4"
/locus_tag="YPL154C"
/gene_synonym="PHO9; PRA1; yscA"
/db_xref="GeneID:855949"
mRNA complement(<259714..>260931)
/gene="PEP4"
/locus_tag="YPL154C"
/gene_synonym="PHO9; PRA1; yscA"
/product="proteinase A"
/transcript_id="NM_001183968.1"
/db_xref="GeneID:855949"
CDS complement(259714..260931)
/gene="PEP4"
/locus_tag="YPL154C"
/gene_synonym="PHO9; PRA1; yscA"
/EC_number="3.4.23.25"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762|PMID:23708375]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0008233 peptidase
activity [PMID:7002931]"
/experiment="EXISTENCE:mutant phenotype:GO:0000425
pexophagy [PMID:31332264]"
/experiment="EXISTENCE:mutant phenotype:GO:0006914
autophagy [PMID:23382696]"
/experiment="EXISTENCE:mutant phenotype:GO:0016236
macroautophagy [PMID:29514932]"
/experiment="EXISTENCE:mutant phenotype:GO:0016237
microautophagy [PMID:12529432]"
/experiment="EXISTENCE:mutant phenotype:GO:0032258
cytoplasm to vacuole targeting by the Cvt pathway
[PMID:19793921]"
/experiment="EXISTENCE:mutant phenotype:GO:0051603
proteolysis involved in protein catabolic process
[PMID:16183335]"
/note="Vacuolar aspartyl protease (proteinase A); required
for post-translational precursor maturation of vacuolar
proteinases; important for protein turnover after
oxidative damage; plays a protective role in acetic acid
induced apoptosis; synthesized as a zymogen,
self-activates; targeted to vacuole via Vps10p-dependent
endosomal vacuolar protein sorting pathway"
/codon_start=1
/product="proteinase A"
/protein_id="NP_015171.1"
/db_xref="GeneID:855949"
/db_xref="SGD:S000006075"
/translation="MFSLKALLPLALLLVSANQVAAKVHKAKIYKHELSDEMKEVTFE
QHLAHLGQKYLTQFEKANPEVVFSREHPFFTEGGHDVPLTNYLNAQYYTDITLGTPPQ
NFKVILDTGSSNLWVPSNECGSLACFLHSKYDHEASSSYKANGTEFAIQYGTGSLEGY
ISQDTLSIGDLTIPKQDFAEATSEPGLTFAFGKFDGILGLGYDTISVDKVVPPFYNAI
QQDLLDEKRFAFYLGDTSKDTENGGEATFGGIDESKFKGDITWLPVRRKAYWEVKFEG
IGLGDEYAELESHGAAIDTGTSLITLPSGLAEMINAEIGAKKGWTGQYTLDCNTRDNL
PDLIFNFNGYNFTIGPYDYTLEVSGSCISAITPMDFPEPVGPLAIVGDAFLRKYYSIY
DLGNNAVGLAKAI"
rep_origin 260931..261727
/note="ARS1612; Putative replication origin; identified in
multiple array studies, not yet confirmed by plasmid-based
assay"
/db_xref="SGD:S000130198"
gene complement(<261727..>264192)
/gene="RAD53"
/locus_tag="YPL153C"
/gene_synonym="LSD1; MEC2; SPK1"
/db_xref="GeneID:855950"
mRNA complement(<261727..>264192)
/gene="RAD53"
/locus_tag="YPL153C"
/gene_synonym="LSD1; MEC2; SPK1"
/product="serine/threonine/tyrosine protein kinase RAD53"
/transcript_id="NM_001183967.1"
/db_xref="GeneID:855950"
CDS complement(261727..264192)
/gene="RAD53"
/locus_tag="YPL153C"
/gene_synonym="LSD1; MEC2; SPK1"
/EC_number="2.7.12.1"
/experiment="EXISTENCE:direct assay:GO:0003688 DNA
replication origin binding [PMID:16816422]"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0004712 protein
serine/threonine/tyrosine kinase activity
[PMID:30377154|PMID:8355715]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476|PMID:8355715]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:genetic interaction:GO:0000077 DNA
damage checkpoint signaling [PMID:7926756]"
/experiment="EXISTENCE:mutant phenotype:GO:0006270 DNA
replication initiation [PMID:16816422]"
/experiment="EXISTENCE:mutant phenotype:GO:0006281 DNA
repair [PMID:8355715]"
/experiment="EXISTENCE:mutant phenotype:GO:0008104
intracellular protein localization [PMID:18045534]"
/experiment="EXISTENCE:mutant phenotype:GO:0009202
deoxyribonucleoside triphosphate biosynthetic process
[PMID:17188244]"
/experiment="EXISTENCE:mutant phenotype:GO:0042326
negative regulation of phosphorylation [PMID:24130507]"
/experiment="EXISTENCE:mutant phenotype:GO:0043247
telomere maintenance in response to DNA damage
[PMID:24130507]"
/note="DNA damage response kinase; signal transduction
pathway component required for DNA damage and replication
checkpoints, promoting cell cycle arrest and DNA repair;
role in initiation of DNA replication; inhibits gene
gating through NPC protein phosphorylation, to promote
fork stability; activates downstream kinase Dun1p; senses
mtDNA depletion and mitochondrial ROS; relocalizes to
cytosol under hypoxia; contains two FHA domains; human
homolog CHEK2 implicated in breast cancer complements the
null"
/codon_start=1
/product="serine/threonine/tyrosine protein kinase RAD53"
/protein_id="NP_015172.1"
/db_xref="GeneID:855950"
/db_xref="SGD:S000006074"
/translation="MENITQPTQQSTQATQRFLIEKFSQEQIGENIVCRVICTTGQIP
IRDLSADISQVLKEKRSIKKVWTFGRNPACDYHLGNISRLSNKHFQILLGEDGNLLLN
DISTNGTWLNGQKVEKNSNQLLSQGDEITVGVGVESDILSLVIFINDKFKQCLEQNKV
DRIRSNLKNTSKIASPGLTSSTASSMVANKTGIFKDFSIIDEVVGQGAFATVKKAIER
TTGKTFAVKIISKRKVIGNMDGVTRELEVLQKLNHPRIVRLKGFYEDTESYYMVMEFV
SGGDLMDFVAAHGAVGEDAGREISRQILTAIKYIHSMGISHRDLKPDNILIEQDDPVL
VKITDFGLAKVQGNGSFMKTFCGTLAYVAPEVIRGKDTSVSPDEYEERNEYSSLVDMW
SMGCLVYVILTGHLPFSGSTQDQLYKQIGRGSYHEGPLKDFRISEEARDFIDSLLQVD
PNNRSTAAKALNHPWIKMSPLGSQSYGDFSQISLSQSLSQQKLLENMDDAQYEFVKAQ
RKLQMEQQLQEQDQEDQDGKIQGFKIPAHAPIRYTQPKSIEAETREQKLLHSNNTENV
KSSKKKGNGRFLTLKPLPDSIIQESLEIQQGVNPFFIGRSEDCNCKIEDNRLSRVHCF
IFKKRHAVGKSMYESPAQGLDDIWYCHTGTNVSYLNNNRMIQGTKFLLQDGDEIKIIW
DKNNKFVIGFKVEINDTTGLFNEGLGMLQEQRVVLKQTAEEKDLVKKLTQMMAAQRAN
QPSASSSSMSAKKPPVSDTNNNGNNSVLNDLVESPINANTGNILKRIHSVSLSQSQID
PSKKVKRAKLDQTSKGPENLQFS"
gene <264602..>264700
/locus_tag="YPL152W-A"
/db_xref="GeneID:1466519"
mRNA <264602..>264700
/locus_tag="YPL152W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184627.1"
/db_xref="GeneID:1466519"
CDS 264602..264700
/locus_tag="YPL152W-A"
/note="hypothetical protein; identified by gene-trapping,
microarray-based expression analysis, and genome-wide
homology searching"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878180.1"
/db_xref="GeneID:1466519"
/db_xref="SGD:S000028721"
/translation="MMVHLTLKSHLIKEELLWHALSPSYSCRYHGR"
gene <265028..>266104
/gene="RRD2"
/locus_tag="YPL152W"
/gene_synonym="YPA2"
/db_xref="GeneID:855951"
mRNA <265028..>266104
/gene="RRD2"
/locus_tag="YPL152W"
/gene_synonym="YPA2"
/product="peptidylprolyl isomerase RRD2"
/transcript_id="NM_001183966.1"
/db_xref="GeneID:855951"
CDS 265028..266104
/gene="RRD2"
/locus_tag="YPL152W"
/gene_synonym="YPA2"
/EC_number="5.2.1.8"
/experiment="EXISTENCE:direct assay:GO:0000159 protein
phosphatase type 2A complex [PMID:17550305]"
/experiment="EXISTENCE:direct assay:GO:0003755
peptidyl-prolyl cis-trans isomerase activity
[PMID:16380387]"
/experiment="EXISTENCE:mutant phenotype:GO:0006970
response to osmotic stress [PMID:10660069]"
/experiment="EXISTENCE:mutant phenotype:GO:0007052 mitotic
spindle organization [PMID:11262194]"
/experiment="EXISTENCE:mutant phenotype:GO:0019888 protein
phosphatase regulator activity [PMID:12952889]"
/note="Peptidyl-prolyl cis/trans-isomerase; also activates
the phosphotyrosyl phosphatase activity of protein
phosphatase 2A (PP2A); regulates G1 phase progression, the
osmoresponse, microtubule dynamics; subunit of the
Tap42p-Pph21p-Rrd2p complex; protein abundance increases
in response to DNA replication stress"
/codon_start=1
/product="peptidylprolyl isomerase RRD2"
/protein_id="NP_015173.1"
/db_xref="GeneID:855951"
/db_xref="SGD:S000006073"
/translation="MLPEKRLLTPDDMKLWEESPTRAHFTKFIIDLAESVKGHENSQY
KEPISESINSMMNLLSQIKDITQKHPVIKDADSSRFGKVEFRDFYDEVSRNSRKILRS
EFPSLTDEQLEQLSIYLDESWGNKRRIDYGSGHELNFMCLLYGLYSYGIFNLSNDSTN
LVLKVFIEYLKIMRILETKYWLEPAGSHGVWGLDDYHFLPFLFGAFQLTTHKHLKPIS
IHNNELVEMFAHRYLYFGCIAFINKVKSSASLRWHSPMLDDISGVKTWSKVAEGMIKM
YKAEVLSKLPIMQHFYFSEFLPCPDGVSPPRGHIHDGTDKDDECNFEGHVHTTWGDCC
GIKLPSAIAATEMNKKHHKPIPFD"
gene complement(<266180..>267535)
/gene="PRP46"
/locus_tag="YPL151C"
/gene_synonym="NTC50"
/db_xref="GeneID:855952"
mRNA complement(<266180..>267535)
/gene="PRP46"
/locus_tag="YPL151C"
/gene_synonym="NTC50"
/product="mRNA splicing protein PRP46"
/transcript_id="NM_001183965.1"
/db_xref="GeneID:855952"
CDS complement(266180..267535)
/gene="PRP46"
/locus_tag="YPL151C"
/gene_synonym="NTC50"
/experiment="EXISTENCE:direct assay:GO:0005681
spliceosomal complex [PMID:11884590]"
/experiment="EXISTENCE:mutant phenotype:GO:0000398 mRNA
splicing, via spliceosome [PMID:12554883]"
/experiment="EXISTENCE:physical interaction:GO:0000974
Prp19 complex [PMID:12088152]"
/note="Member of the NineTeen Complex (NTC); this complex
contains Prp19p and stabilizes U6 snRNA in catalytic forms
of the spliceosome containing U2, U5, and U6 snRNAs"
/codon_start=1
/product="mRNA splicing protein PRP46"
/protein_id="NP_015174.1"
/db_xref="GeneID:855952"
/db_xref="SGD:S000006072"
/translation="MDGNDHKVENLGDVDKFYSRIRWNNQFSYMATLPPHLQSEMEGQ
KSLLMRYDTYRKESSSFSGEGKKVTLQHVPTDFSEASQAVISKKDHDTHASAFVNKIF
QPEVAEELIVNRYEKLLSQRPEWHAPWKLSRVINGHLGWVRCVAIDPVDNEWFITGSN
DTTMKVWDLATGKLKTTLAGHVMTVRDVAVSDRHPYLFSVSEDKTVKCWDLEKNQIIR
DYYGHLSGVRTVSIHPTLDLIATAGRDSVIKLWDMRTRIPVITLVGHKGPINQVQCTP
VDPQVVSSSTDATVRLWDVVAGKTMKVLTHHKRSVRATALHPKEFSVASACTDDIRSW
GLAEGSLLTNFESEKTGIINTLSINQDDVLFAGGDNGVLSFYDYKSGHKYQSLATREM
VGSLEGERSVLCSTFDKTGLRLITGEADKSIKIWKQDETATKESEPGLAWNPNLSAKR
F"
gene <268188..>270893
/locus_tag="YPL150W"
/db_xref="GeneID:855953"
mRNA <268188..>270893
/locus_tag="YPL150W"
/product="non-specific serine/threonine protein kinase"
/transcript_id="NM_001183964.1"
/db_xref="GeneID:855953"
CDS 268188..270893
/locus_tag="YPL150W"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0006468 protein
phosphorylation [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0036464 cytoplasmic
ribonucleoprotein granule [PMID:25342717]"
/note="Protein kinase of unknown cellular role; binds
phosphatidylinositols and cardiolipin in a large-scale
study"
/codon_start=1
/product="non-specific serine/threonine protein kinase"
/protein_id="NP_015175.1"
/db_xref="GeneID:855953"
/db_xref="SGD:S000006071"
/translation="MVNPVGSSKLEQNNIKSIIGSSYNRLYSQFTSDELTEVGNYKIL
KQIGEGSFGKVYLALHRPTHRKVCLKTSDKNDPNIVREVFYHRQFDFPYITKLYEVIV
TESKVWMALEYCPGKELYDHLLSLRRISLLECGELFAQISGAVYYAHSMHCVHRDLKL
ENILLDKNGNAKLTDFGFTRECMTKTTLETVCGTTVYMAPELIERRTYDGFKIDIWSL
GVILYTLITGYLPFDDDDEAKTKWKIVNEEPKYDAKVIPDDARDLISRLLAKNPGERP
SLSQVLRHPFLQPYGSVVLDQTQKILCRQRSGGTQFKSKLERRLLKRLKQSGVDTQAI
KQSILKKKCDSLSGLWLLLLAQGKKQENCKYPKRSRSVLSVKKVIESATHNDTNGISE
DVLKPSLELSRAASLSKMLNKGSDFVTSMTPVSRKKSKDSAKVLNPTLSKISSQRAYS
HSIAGSPRKSNNFLQKVSSFFKSKKSSNSNSNNSIHTNVSESLIASNRGAPSSGSFLK
KNSGSIQKSRTDTVANPSRTESIGSLNENVAGAIVPRSANNTTLENKKTSGNEIGLKV
APELLLNEHIRIEEPRLKRFKSSISSEISQTSTGNYDSESAENSRSISFDGKVSPPPI
RNRPLSEISQISNDTYISEYSTDGNNSSFKISDTIKPSYIRKGSETTSQYSASSEKMT
NGYGRKFVRRDLSIVSTASSTSERSSRTDSFYDITTATPVVTTDNRRNKNNNLKESVL
PRFGTQRPWTGKRTYTTSRHGKNARRSSKRGLFKITSSNTDSIIQEVSSSEEEDHNVI
YSKGKGLPTPVLQTKGLIENGLNERDEEGDDEYAIHTDGEFSIKPQFSDDVIDKQNHL
PSVKAVATKRSLSEGSNWSSSYLDSDNNRRRVSSLLVEDGGNPTA"
regulatory 271236..271277
/regulatory_class="other"
/note="One of four upstream open reading frames (uORFs) in
5' untranslated region of ATG5 gene, regulate translation"
regulatory 271252..271302
/regulatory_class="other"
/note="One of four upstream open reading frames (uORFs) in
5' untranslated region of ATG5 gene, regulate translation"
regulatory 271299..271307
/regulatory_class="other"
/note="One of four upstream open reading frames (uORFs) in
5' untranslated region of ATG5 gene, regulate translation"
regulatory 271302..271307
/regulatory_class="other"
/note="One of four upstream open reading frames (uORFs) in
5' untranslated region of ATG5 gene, regulate translation"
gene <271310..>272194
/gene="ATG5"
/locus_tag="YPL149W"
/gene_synonym="APG5"
/db_xref="GeneID:855954"
mRNA <271310..>272194
/gene="ATG5"
/locus_tag="YPL149W"
/gene_synonym="APG5"
/product="Atg5p"
/transcript_id="NM_001183963.1"
/db_xref="GeneID:855954"
CDS 271310..272194
/gene="ATG5"
/locus_tag="YPL149W"
/gene_synonym="APG5"
/experiment="EXISTENCE:direct assay:GO:0000407 phagophore
assembly site [PMID:11689437|PMID:18497569]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005776
autophagosome [PMID:15155809]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:11897782|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0008047 enzyme
activator activity [PMID:23503366]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:23064152]"
/experiment="EXISTENCE:direct assay:GO:0019776 Atg8-family
ligase activity [PMID:17986448]"
/experiment="EXISTENCE:direct assay:GO:0034274
Atg12-Atg5-Atg16 complex [PMID:11897782]"
/experiment="EXISTENCE:direct assay:GO:0061908 phagophore
[PMID:23549786]"
/experiment="EXISTENCE:direct assay:GO:0120095
vacuole-isolation membrane contact site [PMID:23549786]"
/experiment="EXISTENCE:direct assay:GO:0140355 cargo
receptor ligand activity [PMID:27879200]"
/experiment="EXISTENCE:mutant phenotype:GO:0000423
mitophagy [PMID:19793921]"
/experiment="EXISTENCE:mutant phenotype:GO:0016236
macroautophagy [PMID:32788210|PMID:8921905]"
/experiment="EXISTENCE:mutant phenotype:GO:0032258
cytoplasm to vacuole targeting by the Cvt pathway
[PMID:10712513]"
/experiment="EXISTENCE:mutant phenotype:GO:0034727
piecemeal microautophagy of the nucleus [PMID:18701704]"
/experiment="EXISTENCE:mutant phenotype:GO:0044804
nucleophagy [PMID:22768199]"
/experiment="EXISTENCE:mutant phenotype:GO:0051365
cellular response to potassium ion starvation
[PMID:32788210]"
/note="Conserved protein involved in autophagy and the Cvt
pathway; undergoes conjugation with Atg12p to form a
complex involved in Atg8p lipidation; Atg5p-Atg12p
conjugate enhances E2 activity of Atg3 by rearranging its
catalytic site, also forms a complex with Atg16p; the
Atg5-Atg12/Atg16 complex binds to membranes and is
essential for autophagosome formation; also involved in
methionine restriction extension of chronological lifespan
in an autophagy-dependent manner"
/codon_start=1
/product="Atg5p"
/protein_id="NP_015176.1"
/db_xref="GeneID:855954"
/db_xref="SGD:S000006070"
/translation="MNDIKQLLWNGELNVLVSIDPSFLMKGSPREIAVLRIRVPRETY
LVNYMPLIWNKIKSFLSFDPLTDSEKYFWFEHNKTPIPWNYPVGVLFDCLAGKSATFT
TSFENQVKDVLTFLRIHLVMGDSLPPTIIPIASSKTQAEKFWFHQWKQVCFILNGSSK
AIMSLSVNEARKFWGSVITRNFQDFIEISNKISSSRPRHIPLIIQTSRTSGTFRISQP
TISMTGVNPTLKDIEGDILDVKEGINGNDVMVICQGIEIPWHMLLYDLYSKLRSFDGF
LYITLVPIKGGDKASSEL"
gene complement(<272294..>272815)
/gene="PPT2"
/locus_tag="YPL148C"
/db_xref="GeneID:855955"
mRNA complement(<272294..>272815)
/gene="PPT2"
/locus_tag="YPL148C"
/product="holo-[acyl-carrier-protein] synthase"
/transcript_id="NM_001183962.1"
/db_xref="GeneID:855955"
CDS complement(272294..272815)
/gene="PPT2"
/locus_tag="YPL148C"
/EC_number="2.7.8.7"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0008897
holo-[acyl-carrier-protein] synthase activity
[PMID:9712852]"
/experiment="EXISTENCE:direct assay:GO:0031108
holo-[acyl-carrier-protein] biosynthetic process
[PMID:9712852]"
/experiment="EXISTENCE:mutant phenotype:GO:0005739
mitochondrion [PMID:9712852]"
/note="Phosphopantetheine:protein transferase (PPTase);
activates mitochondrial acyl carrier protein (Acp1p) by
phosphopantetheinylation"
/codon_start=1
/product="holo-[acyl-carrier-protein] synthase"
/protein_id="NP_015177.2"
/db_xref="GeneID:855955"
/db_xref="SGD:S000006069"
/translation="MSFASRNIGRKIAGVGVDIVYLPRFAHILEKYSPFDPCGRSTLN
KITRKFMHEKERFHFSNLLIEENCLTPRLHEYIAGVWALKECSLKALCCCVSKHDLPP
AQVLYAGMLYKTQTDTGVPQLEFDKMFGKKYPKYQQLSKNYDSLFSTHEFLVSLSHDK
DYLIAVTNLVERE"
gene <273255..>275867
/gene="PXA1"
/locus_tag="YPL147W"
/gene_synonym="LPI1; PAL1; PAT2; SSH2"
/db_xref="GeneID:855956"
mRNA <273255..>275867
/gene="PXA1"
/locus_tag="YPL147W"
/gene_synonym="LPI1; PAL1; PAT2; SSH2"
/product="ATP-binding cassette long-chain fatty acid
transporter PXA1"
/transcript_id="NM_001183961.1"
/db_xref="GeneID:855956"
CDS 273255..275867
/gene="PXA1"
/locus_tag="YPL147W"
/gene_synonym="LPI1; PAL1; PAT2; SSH2"
/experiment="EXISTENCE:direct assay:GO:0005778 peroxisomal
membrane [PMID:35127709|PMID:8670886]"
/experiment="EXISTENCE:direct assay:GO:0015867 ATP
transport [PMID:35127709]"
/experiment="EXISTENCE:direct assay:GO:0042626
ATPase-coupled transmembrane transporter activity
[PMID:35127709]"
/experiment="EXISTENCE:mutant phenotype:GO:0015908 fatty
acid transport [PMID:8670886]"
/experiment="EXISTENCE:mutant phenotype:GO:0042626
ATPase-coupled transmembrane transporter activity
[PMID:8670886]"
/note="Subunit of heterodimeric peroxisomal ABC transport
complex, with Pxa2p; required for import of long-chain
fatty acids into peroxisomes; similar to human
adrenoleukodystrophy transporters ABCD1and ABCD2, and
ALD-related proteins; mutations in ABCD1 cause X-linked
adrenoleukodystrophy (X-ALD), a peroxisomal disorder;
human ABCD1 and ABCD2 can each partially complement yeast
pxa1 pxa2 double null mutant"
/codon_start=1
/product="ATP-binding cassette long-chain fatty acid
transporter PXA1"
/protein_id="NP_015178.1"
/db_xref="GeneID:855956"
/db_xref="SGD:S000006068"
/translation="MSTTLAAPAKLKSLLLNLHTHCIGLHVNDVTPKVYFKLLIRHLL
QISRSNAAHPKLRRRAQILLVSLFLSGVTLFSGVTYSTFKIILKCYKFYKFPWKRRNR
RPLIRRTRSQMQLDSGARIMYIPEVELVDRQSPDDNKFMNATDKKKRKRIFIPPKDND
VYEHDKFLFKNVELERAKNSQLFYSKFLNQMNVLSKILIPTVFDKNFLLLTAQIFFLV
MRTWLSLFVAKLDGQIVKNIIAGRGRSFLWDLGCWFLIAVPASYTNSAIKLLQRKLSL
NFRVNLTRYIHDMYLDKRLTFYKLIFDAKASNSVIKNIDNSITNDVAKFCDATCSVFA
NIAKPVIDLIFFSVYLRDNLGTVGVAGIFVNYFITGFILRKYTPPLGKLAGERSASDG
DYYNYHLNMINNSEEIAFYQGTAVERTKVKELYDVLMEKMLLVDKVKFGYNMLEDYVL
KYTWSGLGYVFASIPIVMSTLATGINSEEKNMKEFIVNKRLMLSLADAGSRLMHSIKD
ISQLTGYTNRIFTLLSVLHRVHSLNFNYGAVPSILSIRTEDASRNSNLLPTTDNSQDA
IRGTIQRNFNGIRLENIDVIIPSVRASEGIKLINKLTFQIPLHIDPITSKSNSIQDLS
KANDIKLPFLQGSGSSLLILGPNGCGKSSIQRIIAEIWPVYNKNGLLSIPSENNIFFI
PQKPYFSRGGTLRDQIIYPMSSDEFFDRGFRDKELVQILVEVKLDYLLKRGVGLTYLD
AIADWKDLLSGGEKQRVNFARIMFHKPLYVVLDEATNAISVDMEDYLFNLLKRYRFNF
ISISQRPTLIKYHEMLLEIGENRDGKWQLQAVGTDEAITSIDNEIEELERKLERVKGW
EDERTKLREKLEII"
gene complement(<276162..>277529)
/gene="NOP53"
/locus_tag="YPL146C"
/db_xref="GeneID:855957"
mRNA complement(<276162..>277529)
/gene="NOP53"
/locus_tag="YPL146C"
/product="Nop53p"
/transcript_id="NM_001183960.1"
/db_xref="GeneID:855957"
CDS complement(276162..277529)
/gene="NOP53"
/locus_tag="YPL146C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11914276|PMID:16043506|PMID:10684247]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:10684247|PMID:16043506]"
/experiment="EXISTENCE:direct assay:GO:0019843 rRNA
binding [PMID:16128814]"
/experiment="EXISTENCE:mutant phenotype:GO:0000055
ribosomal large subunit export from nucleus
[PMID:16043506]"
/experiment="EXISTENCE:mutant phenotype:GO:0000176 nuclear
exosome (RNase complex) [PMID:31662437]"
/experiment="EXISTENCE:mutant phenotype:GO:0000463
maturation of LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15686447]"
/experiment="EXISTENCE:mutant phenotype:GO:0006364 rRNA
processing [PMID:16128814]"
/note="Nucleolar protein; involved in biogenesis of the
60S subunit of the ribosome; interacts with rRNA
processing factors Cbf5p and Nop2p and with the nucleolar
proteins Nop17p and Nip7p; null mutant is viable but
growth is severely impaired"
/codon_start=1
/product="Nop53p"
/protein_id="NP_015179.1"
/db_xref="GeneID:855957"
/db_xref="SGD:S000006067"
/translation="MAPTNLTKKPSQYKQSSRKGKKAWRKNIDLSDVEQYMEKKIDHE
ITHGTSDITSLQNDALFHVDVEGDEILKNKLIKRKQIKKVLKSKEILDAVKTNSKIAA
LNHHKNSSGNPNKIQGVSKHELKKLMALAGRVHGESKIKNRVAKDGLVKTTAGDLWGE
ESNSKKQKVKLPSGIKLDVEKKDQIPEELLKKSTTSWSTASVRPSTLDIEPIAVKEFT
EIPHAGKSYNPNNKAWSELINKEYKEEKAREDERIALEKYKERIRHLMETLDDNEEEE
SSSNEEEEEEEEENENENESTQCSGSDKEIKLSINKPVKNKKKTKYQRNKAKRHEEKV
KLQQELKELRQRVKDLEEVINSEETEILSAIESDSNKVKKSKKNKKHKLGTKYSVIDE
RLEIKFSDELSDSLRKLKPEGNLLYDTVRKLQSSGKVETRVPVRKGRKYKQKITEKWT
HKDFK"
gene complement(<278395..>279699)
/gene="KES1"
/locus_tag="YPL145C"
/gene_synonym="BSR3; LPI3; OSH4"
/db_xref="GeneID:855958"
mRNA complement(<278395..>279699)
/gene="KES1"
/locus_tag="YPL145C"
/gene_synonym="BSR3; LPI3; OSH4"
/product="oxysterol-binding protein KES1"
/transcript_id="NM_001183959.1"
/db_xref="GeneID:855958"
CDS complement(278395..279699)
/gene="KES1"
/locus_tag="YPL145C"
/gene_synonym="BSR3; LPI3; OSH4"
/experiment="EXISTENCE:direct assay:GO:0000139 Golgi
membrane [PMID:11916983]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:8978672|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0008289 lipid
binding [PMID:20008566]"
/experiment="EXISTENCE:direct assay:GO:0015918 sterol
transport [PMID:16585271|PMID:20008566]"
/experiment="EXISTENCE:direct assay:GO:0070273
phosphatidylinositol-4-phosphate binding [PMID:20729555]"
/experiment="EXISTENCE:direct assay:GO:0070300
phosphatidic acid binding [PMID:20729555]"
/experiment="EXISTENCE:direct assay:GO:0120015 sterol
transfer activity [PMID:20008566]"
/experiment="EXISTENCE:genetic interaction:GO:0006887
exocytosis [PMID:17004323]"
/experiment="EXISTENCE:genetic interaction:GO:0006892
post-Golgi vesicle-mediated transport
[PMID:11916983|PMID:8978672]"
/experiment="EXISTENCE:genetic interaction:GO:0006897
endocytosis [PMID:15173322]"
/experiment="EXISTENCE:genetic interaction:GO:0015918
sterol transport [PMID:16585271]"
/experiment="EXISTENCE:genetic interaction:GO:0030011
maintenance of cell polarity [PMID:17004323]"
/experiment="EXISTENCE:genetic interaction:GO:0034727
piecemeal microautophagy of the nucleus [PMID:15367582]"
/experiment="EXISTENCE:mutant phenotype:GO:0005546
phosphatidylinositol-4,5-bisphosphate binding
[PMID:11916983]"
/experiment="EXISTENCE:mutant phenotype:GO:0006665
sphingolipid metabolic process [PMID:23593226]"
/experiment="EXISTENCE:mutant phenotype:GO:0008142
oxysterol binding [PMID:16136145]"
/experiment="EXISTENCE:mutant phenotype:GO:0008289 lipid
binding [PMID:20008566]"
/experiment="EXISTENCE:mutant phenotype:GO:0015918 sterol
transport [PMID:20008566]"
/experiment="EXISTENCE:mutant phenotype:GO:0035621 ER to
Golgi ceramide transport [PMID:24213531]"
/experiment="EXISTENCE:mutant phenotype:GO:0120015 sterol
transfer activity [PMID:20008566]"
/note="Sterol/phosphatidylinositol-4-phosphate (PI(4)P)
exchanger; one of seven members of the yeast oxysterol
binding protein family; involved in negative regulation of
Sec14p-dependent Golgi complex secretory functions,
peripheral membrane protein that localizes to the Golgi
complex; KES1 has a paralog, HES1, that arose from the
whole genome duplication"
/codon_start=1
/product="oxysterol-binding protein KES1"
/protein_id="NP_015180.1"
/db_xref="GeneID:855958"
/db_xref="SGD:S000006066"
/translation="MSQYASSSSWTSFLKSIASFNGDLSSLSAPPFILSPISLTEFSQ
YWAEHPELFLEPSFINDDNYKEHCLIDPEVESPELARMLAVTKWFISTLKSQYCSRNE
SLGSEKKPLNPFLGELFVGKWENKEHPEFGETVLLSEQVSHHPPVTAFSIFNDKNKVK
LQGYNQIKASFTKSLMLTVKQFGHTMLDIKDESYLVTPPPLHIEGILVASPFVELEGK
SYIQSSTGLLCVIEFSGRGYFSGKKNSFKARIYKDSKDSKDKEKALYTISGQWSGSSK
IIKANKKEESRLFYDAARIPAEHLNVKPLEEQHPLESRKAWYDVAGAIKLGDFNLIAK
TKTELEETQRELRKEEEAKGISWQRRWFKDFDYSVTPEEGALVPEKDDTFLKLASALN
LSTKNAPSGTLVGDKEDRKEDLSSIHWRFQRELWDEEKEIVL"
gene <280480..>280926
/gene="POC4"
/locus_tag="YPL144W"
/gene_synonym="DMP1; PBA4"
/db_xref="GeneID:855959"
mRNA <280480..>280926
/gene="POC4"
/locus_tag="YPL144W"
/gene_synonym="DMP1; PBA4"
/product="Poc4p"
/transcript_id="NM_001183958.1"
/db_xref="GeneID:855959"
CDS 280480..280926
/gene="POC4"
/locus_tag="YPL144W"
/gene_synonym="DMP1; PBA4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:17707236]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:17707236]"
/experiment="EXISTENCE:genetic interaction:GO:0051131
chaperone-mediated protein complex assembly
[PMID:17707236]"
/experiment="EXISTENCE:mutant phenotype:GO:0043248
proteasome assembly [PMID:18278055|PMID:18260085]"
/experiment="EXISTENCE:mutant phenotype:GO:0051131
chaperone-mediated protein complex assembly
[PMID:17707236]"
/experiment="EXISTENCE:physical interaction:GO:0051131
chaperone-mediated protein complex assembly
[PMID:18278055]"
/note="Component of a heterodimeric Poc4p-Irc25p
chaperone; involved in assembly of alpha subunits into the
20S proteasome; may regulate formation of proteasome
isoforms with alternative subunits under different
conditions; upregulates proteasome assembly in response to
the unfolded protein response activated by mistargeting of
proteins (UPRam)"
/codon_start=1
/product="Poc4p"
/protein_id="NP_015181.1"
/db_xref="GeneID:855959"
/db_xref="SGD:S000006065"
/translation="MLVKTISRTIESESGFLQPTLDVIATLPADDRSKKIPISLVVGF
KQEASLNSSSSLSCYYYAIPLMRDRHINLKSGGSNVVGIPLLDTKDDRIRDMARHMAT
IISERFNRPCYVTWSSLPSEDPSMLVANHLYILKKCLDLLKTELGE"
gene complement(281056..281517)
/gene="SNR17B"
/locus_tag="YNCP0005C"
/db_xref="GeneID:9164985"
ncRNA complement(281056..281517)
/ncRNA_class="snoRNA"
/gene="SNR17B"
/locus_tag="YNCP0005C"
/product="SNR17B"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:3327689]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:3327689|PMID:8455623]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911|PMID:15231838]"
/experiment="EXISTENCE:direct assay:GO:0031428 box C/D
methylation guide snoRNP complex [PMID:10094313]"
/experiment="EXISTENCE:direct assay:GO:0032040
small-subunit processome [PMID:12068309]"
/experiment="EXISTENCE:genetic interaction:GO:0000447
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from
5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:7556076]"
/experiment="EXISTENCE:genetic interaction:GO:0000472
endonucleolytic cleavage to generate mature 5'-end of
SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:7556076]"
/experiment="EXISTENCE:genetic interaction:GO:0000480
endonucleolytic cleavage in 5'-ETS of tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:7556076]"
/experiment="EXISTENCE:genetic interaction:GO:0003723 RNA
binding [PMID:7556076]"
/experiment="EXISTENCE:genetic interaction:GO:0019843 rRNA
binding [PMID:7556076]"
/experiment="EXISTENCE:mutant phenotype:GO:0019843 rRNA
binding [PMID:7556076]"
/experiment="EXISTENCE:physical interaction:GO:0003723 RNA
binding [PMID:3327689]"
/experiment="EXISTENCE:physical interaction:GO:0031428 box
C/D methylation guide snoRNP complex [PMID:10733567]"
/note="Small nucleolar RNA (snoRNA) U3; part of small
(ribosomal) subunit (SSU) processome; SNR17A also encodes
snoRNA U3"
/transcript_id="NR_132267.1"
/db_xref="GeneID:9164985"
/db_xref="SGD:S000007441"
gene <282122..>282970
/gene="RPL33A"
/locus_tag="YPL143W"
/gene_synonym="GCD17; RPL37A"
/db_xref="GeneID:855960"
mRNA join(<282122..282140,282666..>282970)
/gene="RPL33A"
/locus_tag="YPL143W"
/gene_synonym="GCD17; RPL37A"
/product="60S ribosomal protein eL33 RPL33A"
/transcript_id="NM_001183957.1"
/db_xref="GeneID:855960"
CDS join(282122..282140,282666..282970)
/gene="RPL33A"
/locus_tag="YPL143W"
/gene_synonym="GCD17; RPL37A"
/experiment="EXISTENCE:direct assay:GO:0002181 cytoplasmic
translation [PMID:18782943]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:18782943]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:18782943]"
/experiment="EXISTENCE:mutant phenotype:GO:0000027
ribosomal large subunit assembly [PMID:17548477]"
/experiment="EXISTENCE:mutant phenotype:GO:0006364 rRNA
processing [PMID:17548477]"
/note="Ribosomal 60S subunit protein L33A; N-terminally
acetylated; rpl33a null mutant exhibits slow growth while
rpl33a rpl33b double null mutant is inviable; homologous
to mammalian ribosomal protein L35A, no bacterial homolog;
RPL33A has a paralog, RPL33B, that arose from the whole
genome duplication"
/codon_start=1
/product="60S ribosomal protein eL33 RPL33A"
/protein_id="NP_015182.1"
/db_xref="GeneID:855960"
/db_xref="SGD:S000006064"
/translation="MAESHRLYVKGKHLSYQRSKRVNNPNVSLIKIEGVATPQDAQFY
LGKRIAYVYRASKEVRGSKIRVMWGKVTRTHGNSGVVRATFRNNLPAKTFGASVRIFL
YPSNI"
gene complement(<283464..>286061)
/gene="FRK1"
/locus_tag="YPL141C"
/db_xref="GeneID:855962"
mRNA complement(<283464..>286061)
/gene="FRK1"
/locus_tag="YPL141C"
/product="protein kinase FRK1"
/transcript_id="NM_001183955.1"
/db_xref="GeneID:855962"
CDS complement(283464..286061)
/gene="FRK1"
/locus_tag="YPL141C"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:22289182]"
/experiment="EXISTENCE:genetic interaction:GO:0000011
vacuole inheritance [PMID:37509134]"
/experiment="EXISTENCE:genetic interaction:GO:0045033
peroxisome inheritance [PMID:37509134]"
/note="Protein kinase; minor role relative to Kin4p in
regulating peroxisomal transport and vacuolar inheritance;
protects the peroxisomal Myo2p receptor Inp2p from
degradation in mother cells along with Kin4p; green
fluorescent protein (GFP)-fusion protein localizes to the
cytoplasm; interacts with rRNA transcription and ribosome
biogenesis factors and the long chain fatty acyl-CoA
synthetase Faa3p; FRK1 has a paralog, KIN4, that arose
from the whole genome duplication"
/codon_start=1
/product="protein kinase FRK1"
/protein_id="NP_015184.1"
/db_xref="GeneID:855962"
/db_xref="SGD:S000006062"
/translation="MSYTNKRHTYYGGFTNDLSDTFQYPQRTDEQRRKHVTFGPYILG
STLGEGEFGKVKLGWPKNFSNSSNSTFDFPKQVAIKLIKRDSISNDYRKEVKIYREIN
ALKHLSHPNIVKLEEVLQNSRYIGIVLEYACGGEFYKYIQKKRRLKEMNACRLFSQLI
SGVHYIHSKGLVHRDLKLENLLLDKNENLVITDFGFVNEFCSRNELMKTSCGSPCYAA
PELVISAEPYEARKADIWSCGVILYAILAGYLPWDDDPNNPEGSDIGRLYNYINSTPL
KFPDYILPIPRDLLRRMLVSDPKKRINLKQIKKHEWLKPHSSFLSITPDEWDKLNNTQ
SVFRLAKPRRRYGSRPQSSCSTSSLGSRSDKRDSLVIDSTLITFPAPPQESQNHIITR
PASIASDQRLSPIRRSNRHNRSNSAASVALQAVVNADREYVLSHEQSLSPVQNIRQTT
GNMTASLSPPPAISPGDIIIETTPIKRNTISGSSIVPSLEEESSTTMQTSKIQPNNMA
SSQNHQYNKNKTQNSLQSAKNFYRTSSSSHTKPRPTSYHPGSYTTPPYNSNTLSIYEI
NEKAKSSASSQTLNQRDTSPFDSTPYLALDTCITSSSSIESSPKLITHGQFSVAKPSV
DLQSVSGDLIKYKRDADVVTRIYDEKYKQKRKSLRYSGIFSDISCDTVTEESDELRPP
ESPLQQHEGQESIDKAKTEDTSEKGSKSSNIAKATAQKHVNNHLERSLNEAESTKKRF
SFLSLYSYDTSKSSLYSSMDSKRKPSPPSQRRPKKDDSYQTNSKNHYITASNMQTSHQ
VSKDLPAPTMVQNKCTLETKKAVRSNRSSIMVSEVNKASVDNKAAQSPEHSTAKRVLG
FFKRRSMKI"
gene complement(<287514..>289034)
/gene="MKK2"
/locus_tag="YPL140C"
/gene_synonym="LPI6; SSP33"
/db_xref="GeneID:855963"
mRNA complement(<287514..>289034)
/gene="MKK2"
/locus_tag="YPL140C"
/gene_synonym="LPI6; SSP33"
/product="putative mitogen-activated protein kinase kinase
MKK2"
/transcript_id="NM_001183954.1"
/db_xref="GeneID:855963"
CDS complement(287514..289034)
/gene="MKK2"
/locus_tag="YPL140C"
/gene_synonym="LPI6; SSP33"
/EC_number="2.7.12.2"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:12361575]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:12361575]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:12361575]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:12361575]"
/experiment="EXISTENCE:genetic interaction:GO:0000196 cell
integrity MAPK cascade [PMID:8386320]"
/experiment="EXISTENCE:genetic interaction:GO:0000425
pexophagy [PMID:20385774]"
/experiment="EXISTENCE:genetic interaction:GO:0060237
regulation of fungal-type cell wall organization
[PMID:8386320]"
/experiment="EXISTENCE:mutant phenotype:GO:0007165 signal
transduction [PMID:8386320]"
/note="MAPKK involved in the protein kinase C signaling
pathway; involved in control of cell integrity; upon
activation by Bck1p phosphorylates downstream target,
Slt2p; functionally redundant with Mkk1p; MKK2 has a
paralog, MKK1, that arose from the whole genome
duplication"
/codon_start=1
/product="putative mitogen-activated protein kinase kinase
MKK2"
/protein_id="NP_015185.1"
/db_xref="GeneID:855963"
/db_xref="SGD:S000006061"
/translation="MASMFRPPESNRSHQKTPKLTLPVNLVQNAKSTNDGQHLNRSPY
SSVNESPYSNNSTSATSTTSSMASNSTLLYNRSSTTTIKNRPVPPPLPPLVLTQKKDG
IEYRVAGDSQLSERFSNLHVDITYKELLSSAPISTKLSNIDTTFIKKDLDTPEGEDSY
PSTLLSAYDFSSSGSNSAPLSANNIISCSNLIQGKDVDQLEEEAWRFGHLKDEITTLG
ILGEGAGGSVAKCRLKNGKKVFALKTINTMNTDPEYQKQIFRELQFNKSFKSDYIVQY
YGMFTDEQSSSIYIAMEYMGGKSLEATYKNLLKRGGRISERVIGKIAESVLRGLSYLH
ERKVIHRDIKPQNILLNEKGEIKLCDFGVSGEAVNSLAMTFTGTSFYMAPERIQGQPY
SVTCDVWSLGLTLLEVAGGRFPFESDKITQNVAPIELLTMILTFSPQLKDEPELDISW
SKTFRSFIDYCLKKDARERPSPRQMLKHPWIVGQMKKKVNMERFVKKCWEKEKDGI"
rep_origin 289485..289705
/note="ARS1614; Autonomously Replicating Sequence"
/db_xref="SGD:S000118414"
gene complement(<289669..>291051)
/gene="UME1"
/locus_tag="YPL139C"
/gene_synonym="WTM3"
/db_xref="GeneID:855964"
mRNA complement(<289669..>291051)
/gene="UME1"
/locus_tag="YPL139C"
/gene_synonym="WTM3"
/product="Ume1p"
/transcript_id="NM_001183953.1"
/db_xref="GeneID:855964"
CDS complement(289669..291051)
/gene="UME1"
/locus_tag="YPL139C"
/gene_synonym="WTM3"
/experiment="EXISTENCE:direct assay:GO:0003714
transcription corepressor activity [PMID:9234739]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:25398614|PMID:9234739]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:25398614]"
/experiment="EXISTENCE:direct assay:GO:0032221 Rpd3S
complex [PMID:16286007|PMID:16286008]"
/experiment="EXISTENCE:direct assay:GO:0033698 Rpd3L
complex
[PMID:16314178|PMID:16286008|PMID:19040720|PMID:16286007]"
/experiment="EXISTENCE:direct assay:GO:0070210
Rpd3L-Expanded complex [PMID:19040720]"
/experiment="EXISTENCE:mutant phenotype:GO:0016479
negative regulation of transcription by RNA polymerase I
[PMID:19270272]"
/experiment="EXISTENCE:mutant phenotype:GO:0034605
cellular response to heat [PMID:20398213]"
/experiment="EXISTENCE:mutant phenotype:GO:0040020
regulation of meiotic nuclear division [PMID:9234739]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:20398213|PMID:17210643]"
/note="Component of both the Rpd3S and Rpd3L histone
deacetylase complexes; negative regulator of meiosis;
required for repression of a subset of meiotic genes
during vegetative growth, binding of histone deacetylase
Rpd3p required for activity, contains a NEE box and a WD
repeat motif; homologous with Wtm1p; UME1 has a paralog,
WTM2, that arose from the whole genome duplication"
/codon_start=1
/product="Ume1p"
/protein_id="NP_015186.1"
/db_xref="GeneID:855964"
/db_xref="SGD:S000006060"
/translation="MSTLDIAEDNKIKNEEFKIWKKSIPSLYQHISSLKPIFGSGVDE
SPSTLRSIVFTNDSSCNKSKGVLSVPLLYSQGSEIFEVDCIVPLGLHYKKPESISEPL
VQPDYTMESQKVEQTVLIPKWEFKGETIAKMIYVDNSEINVKVIALSTNGSLAWFREG
VKSPVYTMMEPSTSLSSASSGNQNKPCVDFAISNDSKTLTVTKEKHLDNENATIKLID
NSGKIGEVLRTIPVPGIKNIQEIKFLNNQIFATCSDDGIIRFWGNEIGKKPLWILNDS
LDGKTTCFAASPFVDTLFMTGTSGGALKVWDIRAVIALGDADAELNINQGHNKVNELF
KVHHFYSEQVSKIEFSSISPMEVVTIGGLGNVYHWNFEPVFAIYNEIHEDFQGIISDE
LEAESMAFYHTEGCRREIGENNKVNTVAYHKYIEDLVATVDSDGLLTVYKPFTGKVLD
GSREVGAAKS"
gene complement(<291366..>292427)
/gene="SPP1"
/locus_tag="YPL138C"
/gene_synonym="CPS40; SAF41"
/db_xref="GeneID:855965"
mRNA complement(<291366..>292427)
/gene="SPP1"
/locus_tag="YPL138C"
/gene_synonym="CPS40; SAF41"
/product="Spp1p"
/transcript_id="NM_001183952.1"
/db_xref="GeneID:855965"
CDS complement(291366..292427)
/gene="SPP1"
/locus_tag="YPL138C"
/gene_synonym="CPS40; SAF41"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:17898715]"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:18845545]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0042800 histone
H3K4 methyltransferase activity
[PMID:11742990|PMID:11805083]"
/experiment="EXISTENCE:direct assay:GO:0045815
transcription initiation-coupled chromatin remodeling
[PMID:17898715]"
/experiment="EXISTENCE:direct assay:GO:0140002 histone
H3K4me3 reader activity [PMID:17142463]"
/experiment="EXISTENCE:direct assay:GO:0140006 histone H3
reader activity [PMID:17898715]"
/experiment="EXISTENCE:mutant phenotype:GO:0003682
chromatin binding [PMID:23246437]"
/experiment="EXISTENCE:mutant phenotype:GO:0006355
regulation of DNA-templated transcription [PMID:11687631]"
/experiment="EXISTENCE:mutant phenotype:GO:0031509
subtelomeric heterochromatin formation
[PMID:11687631|PMID:11805083|PMID:11752412]"
/experiment="EXISTENCE:mutant phenotype:GO:0042800 histone
H3K4 methyltransferase activity [PMID:11752412]"
/experiment="EXISTENCE:mutant phenotype:GO:1903341
regulation of meiotic DNA double-strand break formation
[PMID:23246437]"
/experiment="EXISTENCE:physical interaction:GO:0048188
Set1C/COMPASS complex
[PMID:11742990|PMID:11687631|PMID:11752412]"
/note="Subunit of COMPASS (Set1C); a complex which
methylates histone H3 on lysine 4 and is required in
telomeric transcriptional silencing; promotes meiotic DSB
formation by interacting with H3K4me3 and Rec107p, a
protein required for Spo11p-catalyzed DSB formation
located on chromosome axes; interacts with Orc2p; PHD
finger domain protein similar to human CGBP, an
unmethylated CpG binding protein; relocalizes to cytosol
in response to hypoxia"
/codon_start=1
/product="Spp1p"
/protein_id="NP_015187.1"
/db_xref="GeneID:855965"
/db_xref="SGD:S000006059"
/translation="MSLPQWCPPHSTLKRNPTTGEDVYCICKRPDYGELMVGCDGCDD
WFHFTCLHIPEQFKDLVFSFYCPYCQAGITGKNKDAIINGEGSLPKTLWKRKCRISDC
YKPCLQDSKYCSEEHGREFVNDIWSRLKTDEDRAVVKKMVEQTGHIDKFKKFGQLDFI
DNNIVVKTDDEKEIFDQIVVRDMTLKTLEDDLQEVQEISLPLFKKKLELLEVYLGWLD
NVYTEMRKLDDDAASHVECGKEDSKGTKRKKKKNSSRSRARKNICGYCSTYERIPCSV
EEFVRDFGSNEEATKIHEVCTKWKCNRHLDWVSTNQEQYLQQIDSLESMQERLQHLIQ
ARKKQLNIQYYEEILRRGL"
gene complement(<292817..>296647)
/gene="GIP3"
/locus_tag="YPL137C"
/db_xref="GeneID:855967"
mRNA complement(<292817..>296647)
/gene="GIP3"
/locus_tag="YPL137C"
/product="protein phosphatase regulator GIP3"
/transcript_id="NM_001183951.1"
/db_xref="GeneID:855967"
CDS complement(292817..296647)
/gene="GIP3"
/locus_tag="YPL137C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008157 protein
phosphatase 1 binding [PMID:11841235]"
/experiment="EXISTENCE:mutant phenotype:GO:0007059
chromosome segregation [PMID:16537909]"
/experiment="EXISTENCE:mutant phenotype:GO:0019888 protein
phosphatase regulator activity [PMID:16537909]"
/note="Cytoplasmic protein that regulates protein
phosphatase 1 Glc7p; overexpression relocalizes Glc7p from
the nucleus and prevents chromosome segregation; may
interact with ribosomes, based on co-purification
experiments; GIP3 has a paralog, HER1, that arose from the
whole genome duplication"
/codon_start=1
/product="protein phosphatase regulator GIP3"
/protein_id="NP_015188.1"
/db_xref="GeneID:855967"
/db_xref="SGD:S000006058"
/translation="MITNTEFDVPVDWLYKGKSRRKTNTKPSRPSTSPASSSSTSSSK
NGDNSTSGNRSSNDKPRARSSSVSNAALCNTEKPDLKRNDGNTSASDTDNIPLLTPIN
SGNRSDSADIDNPATVDAIDLIDNDDNGSSTQFVRKKRSTSISNAVVSSKPRLASSAI
NATASSSVGKGKHPPISSPSNATLKRSNSTSGEKTKRSIFGSLFSKRSTSSSASTAKK
PLPVVNTSTTENESGGIKAVATPDPRVKEISSPMRGVAPTASKPQTPILPSPALAVKD
LSTVSLKRVSFAVDKFESDPPQQLPSRTPKKGNILIPDDMISEVPSISVGISSSNQSA
KSTNSNIKGPLYTKKSKEYILALENQKLALREAAKHQQEAHFAANRIAFEVANFKTAS
DAGGKLTEKSSEGTITKQREEVSPPNVEADRELENNKLAENLSKAGIDKPIHMHEHYF
KEPDQDKYQDGHSIENNEVTLDVIYTRCCHLREILPIPSTLRQVKDKTAPLQILKFLN
PKPTLIDILSFCDFITIAPIHTIVFDNVALNQDMFRIIISALVNSTVLDKLSLRNVRI
DQDGWKLLCKFLLLNKSLNKLDISQTKIKSDLAESLYRHNMDWNLFTDVLSQRSHKPI
EELLFNGIQFSKIPYSCFARLLTSFATQKNFPESGIRLGLAGATTSNISQDCLKFIFN
WMSQYNVQGVDLAFNDLSTMIKPMVGKLSALSYDNLRYFILNSTNISTSYDLALLLKY
LSKLPNLIFLDLSNLSQCFPDILPYMYKYLPRFPNLKRIHLDSNNLTLKELAVVCNIL
IKCKSLSHVSMTNQNVENFYLMNGTDSPVQQTNTDGDLDSSSTLDVKGQFAKNSFSST
LYAFARDSPNLIGLDFDYDLISEEIQSRIALCLMRNMKRTMDSTFQLDELDSQDDLLF
DGSLVTMTAESVLEKLNLLSDKSTKVKKDTTKRYLLKKYIEKFHILHHNVQHTIDTMF
EKRKSGELPLQEKENLVRLLLLEQNLCNILELFSHNPNLNDVLGSSRDDSKESVDSSE
DSKLPALKHVESGYHVPEEKIQPENDVITARPHLMATDSGKTIDVFTGKPLVFKHTSS
STSVGCKKQEEEEGELHKWGFFVQQQRSLYPENESTRQTPFASGDTPINTETAGKSTS
SPSVSTSNNETATTSLFSPANPKILPKIPSGAVLRSAIMKAKGIDSIDDLIQNVNSNN
IELENIYGESIQNSASTFTPGVDSDVSAPNTDKGSVETLPAVSTDDPNCEVKVTATYD
KLLNNLSMERSIRL"
gene <297553..>298050
/gene="ISU1"
/locus_tag="YPL135W"
/gene_synonym="NUA1"
/db_xref="GeneID:855968"
mRNA <297553..>298050
/gene="ISU1"
/locus_tag="YPL135W"
/gene_synonym="NUA1"
/product="iron-binding protein ISU1"
/transcript_id="NM_001183949.1"
/db_xref="GeneID:855968"
CDS 297553..298050
/gene="ISU1"
/locus_tag="YPL135W"
/gene_synonym="NUA1"
/experiment="EXISTENCE:direct assay:GO:0001671 ATPase
activator activity [PMID:12756240]"
/experiment="EXISTENCE:direct assay:GO:0005506 iron ion
binding [PMID:31532427]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:11914276|PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:15143178|PMID:10468587]"
/experiment="EXISTENCE:direct assay:GO:0008198 ferrous
iron binding [PMID:12970193]"
/experiment="EXISTENCE:direct assay:GO:0008270 zinc ion
binding [PMID:31532427]"
/experiment="EXISTENCE:genetic interaction:GO:0002098 tRNA
wobble uridine modification [PMID:17283054]"
/experiment="EXISTENCE:genetic interaction:GO:0016226
iron-sulfur cluster assembly [PMID:12970193]"
/experiment="EXISTENCE:mutant phenotype:GO:0006879
intracellular iron ion homeostasis [PMID:10468587]"
/experiment="EXISTENCE:mutant phenotype:GO:0016226
iron-sulfur cluster assembly
[PMID:31040179|PMID:15143178|PMID:10588895]"
/note="Conserved protein of the mitochondrial matrix;
performs a scaffolding function during assembly of
iron-sulfur clusters, interacts physically and
functionally with yeast frataxin (Yfh1p); ISU1 has a
paralog, ISU2, that arose from the whole genome
duplication; isu1 isu2 double mutant is inviable; human
homolog ISCU implicated in mitochondrial myopathy, can
complement isu1 isu2 double mutant"
/codon_start=1
/product="iron-binding protein ISU1"
/protein_id="NP_015190.1"
/db_xref="GeneID:855968"
/db_xref="SGD:S000006056"
/translation="MLPVITRFARPALMAIRPVNAMGVLRASSITKRLYHPKVIEHYT
HPRNVGSLDKKLPNVGTGLVGAPACGDVMRLQIKVNDSTGVIEDVKFKTFGCGSAIAS
SSYMTELVQGMTLDDAAKIKNTEIAKELSLPPVKLHCSMLAEDAIKAAIKDYKSKRNT
PTMLS"
gene complement(<298571..>299503)
/gene="ODC1"
/locus_tag="YPL134C"
/db_xref="GeneID:855969"
mRNA complement(<298571..>299503)
/gene="ODC1"
/locus_tag="YPL134C"
/product="mitochondrial 2-oxodicarboxylate carrier"
/transcript_id="NM_001183948.1"
/db_xref="GeneID:855969"
CDS complement(298571..299503)
/gene="ODC1"
/locus_tag="YPL134C"
/experiment="EXISTENCE:direct assay:GO:0005310
dicarboxylic acid transmembrane transporter activity
[PMID:11013234]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:9178508|PMID:14576278|PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:11013234]"
/experiment="EXISTENCE:direct assay:GO:0006839
mitochondrial transport [PMID:11013234]"
/note="Mitochondrial inner membrane transporter;
2-oxodicarboxylate transporter, exports 2-oxoadipate and
2-oxoglutarate from the mitochondrial matrix to the
cytosol for lysine and glutamate biosynthesis and lysine
catabolism; suppresses, in multicopy, an fmc1 null
mutation; ODC1 has a paralog, ODC2, that arose from the
whole genome duplication"
/codon_start=1
/product="mitochondrial 2-oxodicarboxylate carrier"
/protein_id="NP_015191.1"
/db_xref="GeneID:855969"
/db_xref="SGD:S000006055"
/translation="MTSIDNRPLPFIYQFTAGAIAGVSELLVMYPLDVVKTRMQLQVT
TKGHPAVVAAKAAVDHYTGVMDCLTKIVKKEGFSHLYKGITSPILMEAPKRAIKFSGN
DTFQTFYKKIFPTPNGEMTQKIAIYSGASAGAVEAFVVAPFELVKIRLQDVNSQFKTP
IEVVKNSVVKGGVLSLFNGLEATIWRHVLWNAGYFGIIFQIRKLLPAAKTSTEKTRND
LIAGAIGGTVGCLLNTPFDVVKSRIQRSSGPLRKYNWSLPSVLLVYREEGFKALYKGF
APKVMRLAPGGGLLLVVFTNVMDFFREVKYGKKQ"
gene complement(<299888..>301228)
/gene="RDS2"
/locus_tag="YPL133C"
/db_xref="GeneID:855970"
mRNA complement(<299888..>301228)
/gene="RDS2"
/locus_tag="YPL133C"
/product="gluconeogenesis transcription factor RDS2"
/transcript_id="NM_001183947.1"
/db_xref="GeneID:855970"
CDS complement(299888..301228)
/gene="RDS2"
/locus_tag="YPL133C"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:17875938]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:19111667]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:17875938]"
/experiment="EXISTENCE:mutant phenotype:GO:0001228
DNA-binding transcription activator activity, RNA
polymerase II-specific [PMID:17875938]"
/experiment="EXISTENCE:mutant phenotype:GO:0045722
positive regulation of gluconeogenesis [PMID:17875938]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:11943786|PMID:17875938]"
/experiment="EXISTENCE:mutant phenotype:GO:0071466
cellular response to xenobiotic stimulus [PMID:11943786]"
/note="Transcription factor involved in regulating
gluconeogenesis; also involved in the regulation of
glyoxylate cycle genes; member of the zinc cluster family
of proteins; confers resistance to ketoconazole"
/codon_start=1
/product="gluconeogenesis transcription factor RDS2"
/protein_id="NP_015192.1"
/db_xref="GeneID:855970"
/db_xref="SGD:S000006054"
/translation="MSANSGVKRASKAFKTCLFCKRSHVVCDKQRPCSRCVKRDIAHL
CREDDIAVPNEMPSQHESSPNDNNIQGKYANKAHTGIPSDYQNEPVNKSGSTYGEELS
PKLDSSLVNDTTSLLLPQQPVFVSENVGSEFSSLNEFLSMLENPLLTQTSLSSSSASN
VHLENGSQTTQSPLEYQNDNRRDEIGVARQENRSPTIMSGSSNSISKGDKQDQEKEES
RILANANENSAPTPKEQFFLTAADPSTEMTPEHRLKLVINAKLEAGLLKPYNYAKGYA
RLQDYMDKYMNQSSKQRILKPLSTIRPAFRTIARSLKDVDLVLVEESFERMLLSYDRV
FTSMSMPACLCRRTGEIYRANKEFASLVDCTVDDLRDGKLAIYELMTEESAVNFWEKY
GSIAFDKGQKAVLTSCSLRTKDGIRKRPCCFSFTIRRDRYNIPICIVGNFIPLS"
gene <301716..>302618
/gene="COX11"
/locus_tag="YPL132W"
/gene_synonym="LPI13; PSO7"
/db_xref="GeneID:855971"
mRNA <301716..>302618
/gene="COX11"
/locus_tag="YPL132W"
/gene_synonym="LPI13; PSO7"
/product="Cox11p"
/transcript_id="NM_001183946.1"
/db_xref="GeneID:855971"
CDS 301716..302618
/gene="COX11"
/locus_tag="YPL132W"
/gene_synonym="LPI13; PSO7"
/experiment="EXISTENCE:direct assay:GO:0005507 copper ion
binding [PMID:12063264]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239|PMID:2167832]"
/experiment="EXISTENCE:direct assay:GO:0005740
mitochondrial envelope [PMID:2167832]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane
[PMID:15776235|PMID:10501934]"
/experiment="EXISTENCE:direct assay:GO:0005758
mitochondrial intermembrane space [PMID:22984289]"
/experiment="EXISTENCE:direct assay:GO:0005761
mitochondrial ribosome [PMID:15776235]"
/experiment="EXISTENCE:mutant phenotype:GO:0009060 aerobic
respiration [PMID:10617659]"
/experiment="EXISTENCE:mutant phenotype:GO:0045454 cell
redox homeostasis [PMID:34919594]"
/experiment="EXISTENCE:mutant phenotype:GO:0065003
protein-containing complex assembly [PMID:2167832]"
/note="Protein required for delivery of copper to Cox1p;
mitochondrial inner membrane protein; involved in
oxidative stress response; association with mitochondrial
ribosomes suggests that copper delivery may occur during
translation of Cox1p"
/codon_start=1
/product="Cox11p"
/protein_id="NP_015193.1"
/db_xref="GeneID:855971"
/db_xref="SGD:S000006053"
/translation="MIRICPIVRSKVPLLGTFLRSDSWLAPHALALRRAICKNVALRS
YSVNSEQPKHTFDISKLTRNEIQQLRELKRARERKFKDRTVAFYFSSVAVLFLGLAYA
AVPLYRAICARTGFGGIPITDRRKFTDDKLIPVDTEKRIRISFTSEVSQILPWKFVPQ
QREVYVLPGETALAFYKAKNYSDKDIIGMATYSIAPGEAAQYFNKIQCFCFEEQKLAA
GEEIDMPVFFFIDPDFASDPAMRNIDDIILHYTFFRAHYGDGTAVSDSKKEPEMNADE
KAASLANAAILSPEVIDTRKDNSN"
gene <303121..>304014
/gene="RPL5"
/locus_tag="YPL131W"
/gene_synonym="LPI14; RPL1"
/db_xref="GeneID:855972"
mRNA <303121..>304014
/gene="RPL5"
/locus_tag="YPL131W"
/gene_synonym="LPI14; RPL1"
/product="60S ribosomal protein uL18 RPL5"
/transcript_id="NM_001183945.1"
/db_xref="GeneID:855972"
CDS 303121..304014
/gene="RPL5"
/locus_tag="YPL131W"
/gene_synonym="LPI14; RPL1"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:11983894]"
/experiment="EXISTENCE:curator inference:GO:0003735
structural constituent of ribosome [PMID:11983894]"
/experiment="EXISTENCE:direct assay:GO:0008097 5S rRNA
binding [PMID:8764831]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:11983894]"
/experiment="EXISTENCE:mutant phenotype:GO:0000027
ribosomal large subunit assembly [PMID:8474444]"
/note="Ribosomal 60S subunit protein L5; nascent Rpl5p is
bound by specific chaperone Syo1p during translation;
homologous to mammalian ribosomal protein L5 and bacterial
L18; binds 5S rRNA and is required for 60S subunit
assembly"
/codon_start=1
/product="60S ribosomal protein uL18 RPL5"
/protein_id="NP_015194.1"
/db_xref="GeneID:855972"
/db_xref="SGD:S000006052"
/translation="MAFQKDAKSSAYSSRFQTPFRRRREGKTDYYQRKRLVTQHKAKY
NTPKYRLVVRFTNKDIICQIISSTITGDVVLAAAYSHELPRYGITHGLTNWAAAYATG
LLIARRTLQKLGLDETYKGVEEVEGEYELTEAVEDGPRPFKVFLDIGLQRTTTGARVF
GALKGASDGGLYVPHSENRFPGWDFETEEIDPELLRSYIFGGHVSQYMEELADDDEER
FSELFKGYLADDIDADSLEDIYTSAHEAIRADPAFKPTEKKFTKEQYAAESKKYRQTK
LSKEERAARVAAKIAALAGQQ"
gene <304387..>305058
/gene="SPO19"
/locus_tag="YPL130W"
/db_xref="GeneID:855973"
mRNA <304387..>305058
/gene="SPO19"
/locus_tag="YPL130W"
/product="Spo19p"
/transcript_id="NM_001183944.1"
/db_xref="GeneID:855973"
CDS 304387..305058
/gene="SPO19"
/locus_tag="YPL130W"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:18756268]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:10383953]"
/experiment="EXISTENCE:genetic interaction:GO:0051321
meiotic cell cycle [PMID:17179081]"
/experiment="EXISTENCE:genetic interaction:GO:0070583
spore membrane bending pathway [PMID:18756268]"
/experiment="EXISTENCE:mutant phenotype:GO:0070583 spore
membrane bending pathway [PMID:18756268]"
/note="Meiosis-specific prospore protein; required to
produce bending force necessary for proper assembly of the
prospore membrane during sporulation; identified as a weak
high-copy suppressor of the spo1-1 ts mutation; SPO19 has
a paralog, YOR214C, that arose from the whole genome
duplication"
/codon_start=1
/product="Spo19p"
/protein_id="NP_015195.1"
/db_xref="GeneID:855973"
/db_xref="SGD:S000006051"
/translation="MKKQILIVAAQSILCSTVFGERSNVGLSTEELGGDSILYFNEDP
IVIEIDKKAIDKKTLEQLASTRDVVLTDLPDTLEFIDFNEYAKMKSKSDMLLEYINEY
EFDDFERSSEGGLEEEEEEDLIYDFNAQAEDLGKLGSNIYEVVEEKNIVNTYDGNLIN
ASTTESTTTIRPFVTSHSYVASSTPYSNISSLNEDYDNASNFLTPTTVALAVLLTILL
FIQAY"
gene <305298..>306137
/gene="TAF14"
/locus_tag="YPL129W"
/gene_synonym="ANC1; SWP29; TAF30; TFG3"
/db_xref="GeneID:855974"
mRNA join(<305298..305306,305412..>306137)
/gene="TAF14"
/locus_tag="YPL129W"
/gene_synonym="ANC1; SWP29; TAF30; TFG3"
/product="TATA-binding protein-associated factor TAF14"
/transcript_id="NM_001183943.1"
/db_xref="GeneID:855974"
CDS join(305298..305306,305412..306137)
/gene="TAF14"
/locus_tag="YPL129W"
/gene_synonym="ANC1; SWP29; TAF30; TFG3"
/experiment="EXISTENCE:direct assay:GO:0003677 DNA binding
[PMID:35970389]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005669
transcription factor TFIID complex
[PMID:10788514|PMID:15448131]"
/experiment="EXISTENCE:direct assay:GO:0005674
transcription factor TFIIF complex
[PMID:12242279|PMID:15896708|PMID:7995524]"
/experiment="EXISTENCE:direct assay:GO:0006338 chromatin
remodeling [PMID:12887900]"
/experiment="EXISTENCE:direct assay:GO:0006366
transcription by RNA polymerase II
[PMID:12138208|PMID:15448131]"
/experiment="EXISTENCE:direct assay:GO:0006367
transcription initiation at RNA polymerase II promoter
[PMID:35970389|PMID:1331084]"
/experiment="EXISTENCE:direct assay:GO:0016251 RNA
polymerase II general transcription initiation factor
activity [PMID:14749386]"
/experiment="EXISTENCE:direct assay:GO:0016514 SWI/SNF
complex [PMID:18644858]"
/experiment="EXISTENCE:direct assay:GO:0016592 mediator
complex [PMID:8187178]"
/experiment="EXISTENCE:direct assay:GO:0031011 Ino80
complex [PMID:12887900]"
/experiment="EXISTENCE:direct assay:GO:0033100 NuA3
histone acetyltransferase complex
[PMID:10817755|PMID:17157260]"
/experiment="EXISTENCE:direct assay:GO:1990467 NuA3a
histone acetyltransferase complex [PMID:25104842]"
/experiment="EXISTENCE:direct assay:GO:1990468 NuA3b
histone acetyltransferase complex [PMID:25104842]"
/experiment="EXISTENCE:physical interaction:GO:0005674
transcription factor TFIIF complex [PMID:15896708]"
/experiment="EXISTENCE:physical interaction:GO:0031011
Ino80 complex [PMID:24034245]"
/note="Subunit of TFIID, TFIIF, INO80, SWI/SNF, and NuA3
complexes; involved in RNA polymerase II transcription
initiation and in chromatin modification; serves as an
additional DNA binding domain for the complex; contains a
YEATS domain"
/codon_start=1
/product="TATA-binding protein-associated factor TAF14"
/protein_id="NP_015196.1"
/db_xref="GeneID:855974"
/db_xref="SGD:S000006050"
/translation="MVATVKRTIRIKTQQHILPEVPPVENFPVRQWSIEIVLLDDEGK
EIPATIFDKVIYHLHPTFANPNRTFTDPPFRIEEQGWGGFPLDISVFLLEKAGERKIP
HDLNFLQESYEVEHVIQIPLNKPLLTEELAKSGSTEETTANTGTIGKRRTTTNTTAEP
KAKRAKTGSASTVKGSVDLEKLAFGLTKLNEDDLVGVVQMVTDNKTPEMNVTNNVEEG
EFIIDLYSLPEGLLKSLWDYVKKNTE"
gene complement(<306533..>308221)
/gene="TBF1"
/locus_tag="YPL128C"
/gene_synonym="LPI16"
/db_xref="GeneID:855975"
mRNA complement(<306533..>308221)
/gene="TBF1"
/locus_tag="YPL128C"
/gene_synonym="LPI16"
/product="Tbf1p"
/transcript_id="NM_001183942.1"
/db_xref="GeneID:855975"
CDS complement(306533..308221)
/gene="TBF1"
/locus_tag="YPL128C"
/gene_synonym="LPI16"
/experiment="EXISTENCE:direct assay:GO:0000781 chromosome,
telomeric region [PMID:20513435]"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:20513435]"
/experiment="EXISTENCE:direct assay:GO:0000981 DNA-binding
transcription factor activity, RNA polymerase II-specific
[PMID:20513435]"
/experiment="EXISTENCE:direct assay:GO:0001015 snoRNA
transcription by RNA polymerase II [PMID:20513435]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0010833 telomere
maintenance via telomere lengthening [PMID:17917674]"
/experiment="EXISTENCE:direct assay:GO:0042162 telomeric
DNA binding [PMID:20513435]"
/experiment="EXISTENCE:direct assay:GO:0043035 chromatin
insulator sequence binding [PMID:11258704]"
/experiment="EXISTENCE:genetic interaction:GO:0006338
chromatin remodeling [PMID:23222485]"
/experiment="EXISTENCE:genetic interaction:GO:0032211
negative regulation of telomere maintenance via telomerase
[PMID:16467854]"
/experiment="EXISTENCE:mutant phenotype:GO:0001015 snoRNA
transcription by RNA polymerase II [PMID:20513435]"
/experiment="EXISTENCE:mutant phenotype:GO:0006338
chromatin remodeling [PMID:23222485]"
/note="Telobox-containing general regulatory factor; binds
TTAGGG repeats within subtelomeric anti-silencing regions
(STARs), blocking silent chromatin propagation; binds
majority of snoRNA gene promoters, required for full
snoRNA expression; caps DSB flanked by long T2AG3 repeats
and blocks checkpoint activation; involved in nucleosome
displacement during double-strand break repair"
/codon_start=1
/product="Tbf1p"
/protein_id="NP_015197.1"
/db_xref="GeneID:855975"
/db_xref="SGD:S000006049"
/translation="MDSQVPNNNESLNRFNDIIQSLPARTRLTICSLCLLDNISTQLL
RFLILNANSPNIIAVLTDQTAFLSSGETEIFQTLVKLFKQIRMIYHTRSPLLSVHDVA
PGLWFPNSPPPLILRGHEAFIITAIRKANLLTFLLTSLNCLNYGFELLQSIFLDIFCP
NTNTVGNNSLEQSGKFLKSQAILYLDLKTQAYIAGLKEFQDETNEISLEKKQELLDLI
FPSNLADILVQRRTGDSGDITLLTPSEKDFVERCDRRRENLKIVQDFNSLTQSYEWAQ
FIRELLDYCNKNMGLIIWGRKGRGKSPLYDFDVNEFDPQVLFSTGTRTVEFMDDQNQP
SSASAFLSTARPNHYSTHTPTTDVSSKNPAITQSIVDAAVAASMSNSSSGPHSSHNNS
SNSNNNGSIGLRKPKAKRTWSKEEEEALVEGLKEVGPSWSKILDLYGPGGKITENLKN
RTQVQLKDKARNWKLQYLKSGKPLPDYLIKVTGNLEKIYKAKKKFSQSPNSSTIMEQN
LSQHPSSAASATEDTQTHQEDSHGQNSDNMPSNGLFGNSTSDNTGFDPHLEDGM"
gene complement(<308828..>309604)
/gene="HHO1"
/locus_tag="YPL127C"
/db_xref="GeneID:855976"
mRNA complement(<308828..>309604)
/gene="HHO1"
/locus_tag="YPL127C"
/product="histone H1"
/transcript_id="NM_001183941.1"
/db_xref="GeneID:855976"
CDS complement(308828..309604)
/gene="HHO1"
/locus_tag="YPL127C"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:22586276]"
/experiment="EXISTENCE:direct assay:GO:0003677 DNA binding
[PMID:11574687]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11914276|PMID:9046096]"
/experiment="EXISTENCE:mutant phenotype:GO:0006355
regulation of DNA-templated transcription [PMID:11574687]"
/experiment="EXISTENCE:mutant phenotype:GO:0030261
chromosome condensation [PMID:22586276]"
/experiment="EXISTENCE:mutant phenotype:GO:0043934
sporulation [PMID:22586276]"
/experiment="EXISTENCE:mutant phenotype:GO:0045910
negative regulation of DNA recombination [PMID:12820979]"
/experiment="EXISTENCE:mutant phenotype:GO:2000779
regulation of double-strand break repair [PMID:31821952]"
/note="Histone H1, linker histone with roles in meiosis
and sporulation; decreasing levels early in sporulation
may promote meiosis, and increasing levels during
sporulation facilitate compaction of spore chromatin;
binds to promoters and within genes in mature spores; may
be recruited by Ume6p to promoter regions, contributing to
transcriptional repression outside of meiosis; suppresses
DNA repair involving homologous recombination"
/codon_start=1
/product="histone H1"
/protein_id="NP_015198.1"
/db_xref="GeneID:855976"
/db_xref="SGD:S000006048"
/translation="MAPKKSTTKTTSKGKKPATSKGKEKSTSKAAIKKTTAKKEEASS
KSYRELIIEGLTALKERKGSSRPALKKFIKENYPIVGSASNFDLYFNNAIKKGVEAGD
FEQPKGPAGAVKLAKKKSPEVKKEKEVSPKPKQAATSVSATASKAKAASTKLAPKKVV
KKKSPTVTAKKASSPSSLTYKEMILKSMPQLNDGKGSSRIVLKKYVKDTFSSKLKTSS
NFDYLFNSAIKKCVENGELVQPKGPSGIIKLNKKKVKLST"
gene <310210..>312900
/gene="NAN1"
/locus_tag="YPL126W"
/gene_synonym="UTP17"
/db_xref="GeneID:855977"
mRNA <310210..>312900
/gene="NAN1"
/locus_tag="YPL126W"
/gene_synonym="UTP17"
/product="Nan1p"
/transcript_id="NM_001183940.1"
/db_xref="GeneID:855977"
CDS 310210..312900
/gene="NAN1"
/locus_tag="YPL126W"
/gene_synonym="UTP17"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:12068309]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:36164978]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/experiment="EXISTENCE:direct assay:GO:0032040
small-subunit processome [PMID:12068309]"
/experiment="EXISTENCE:direct assay:GO:0033553 rDNA
heterochromatin [PMID:15489292]"
/experiment="EXISTENCE:direct assay:GO:0034455 t-UTP
complex [PMID:17515605]"
/experiment="EXISTENCE:direct assay:GO:0034511 U3 snoRNA
binding [PMID:12068309]"
/experiment="EXISTENCE:mutant phenotype:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12068309]"
/experiment="EXISTENCE:mutant phenotype:GO:0045943
positive regulation of transcription by RNA polymerase I
[PMID:15489292]"
/note="U3 snoRNP protein; component of the small
(ribosomal) subunit (SSU) processosome containing U3
snoRNA; required for the biogenesis of18S rRNA; localizes
to nucleolus and peroxisomes"
/codon_start=1
/product="Nan1p"
/protein_id="NP_015199.1"
/db_xref="GeneID:855977"
/db_xref="SGD:S000006047"
/translation="MTQSLGIEQYKLSVVSGGKPALNNLSSVTGNKNIARLSQDQRNY
IIPFNNQIKVYSVETRQCVKTLKFANNSLLSGIFLQEEENNESIVKILLGDITVPQQE
DAHLITVFTNNGHVIVLNYKGKLVESPKHFKISLADEKLANVFHSEGNYRILTTFKDP
SQKAHNSLQSYRLYALTFDDAKKQFEVAHQAEWHNVILSNISSNGKLLAHMCKDVSTK
DHEHKSISVVSLFDDSVNLSFPLGSILSSQTQSLSYNTRYVSSMAIDNMGQQLAVGFA
SGVISIVSLADLQIRLLKWHIDSVLSLSFSHDGSYLLSGGWEKVMSLWQLETNSQQFL
PRLNGIIIDCQVLGPQGNYYSLILQMTENNSNSDYQFLLLNASDLTSKLSINGPLPVF
NSTIKHIQQPISAMNTKNSNSITSLNHSKKKQSRKLIKSRRQDFTTNVEINPINKNLY
FPHISAVQIFDFYKNEQVNYQYLTSGVNNSMGKVRFELNLQDPIITDLKFTKDGQWMI
TYEIEYPPNDLLSSKDLTHILKFWTKNDNETNWNLKTKVINPHGISVPITKILPSPRS
VNNSQGCLTADNNGGLKFWSFDSHESNWCLKKISLPNFNHFSNSVSLAWSQDGSLIFH
GFDDKLQILDFDTFKKFESLENTKTVSEFTLDSEIQTVKLINDTNLIVATRTTLNAIN
LLRGQVINSFDLYPFVNGVYKNGHMDRLITCDERTGNIALVINQQLTDLDGVPTINYK
SRIIIFDSDLSTKLGNFTHHEYISWIGWNYDTDFIFLDIESTLGVVGTTVNTQLSDEV
NNEGILDGLVSNTITTSASNSDIFAEQLHKLSSRGKKSDTRDKNTNDNDEDEEDIALE
FINGEKKDKLVNMNSFTSMFDNIQNVQMDTFFDRVMKVLT"
gene <313388..>316486
/gene="KAP120"
/locus_tag="YPL125W"
/gene_synonym="LPH2"
/db_xref="GeneID:855978"
mRNA <313388..>316486
/gene="KAP120"
/locus_tag="YPL125W"
/gene_synonym="LPH2"
/product="karyopherin KAP120"
/transcript_id="NM_001183939.1"
/db_xref="GeneID:855978"
CDS 313388..316486
/gene="KAP120"
/locus_tag="YPL125W"
/gene_synonym="LPH2"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10684247|PMID:16581791]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:10684247]"
/experiment="EXISTENCE:direct assay:GO:0006606 protein
import into nucleus [PMID:20219973]"
/experiment="EXISTENCE:direct assay:GO:0008139 nuclear
localization sequence binding [PMID:20219973]"
/experiment="EXISTENCE:direct assay:GO:0061608 nuclear
import signal receptor activity [PMID:20219973]"
/experiment="EXISTENCE:mutant phenotype:GO:0006606 protein
import into nucleus [PMID:20219973|PMID:16581791]"
/experiment="EXISTENCE:mutant phenotype:GO:0061608 nuclear
import signal receptor activity [PMID:20219973]"
/experiment="EXISTENCE:physical interaction:GO:0006606
protein import into nucleus [PMID:16581791]"
/note="Karyopherin responsible for the nuclear import of
Rpf1p; Rpf1p is a ribosome maturation factor"
/codon_start=1
/product="karyopherin KAP120"
/protein_id="NP_015200.1"
/db_xref="GeneID:855978"
/db_xref="SGD:S000006046"
/translation="MASSLNELNLVQVLEQASNPQHIRSDVQKLAEQQLRQWETQAGF
HYLLQSIYLNLSNSLQIRWLAVIQFKNGVDKYWRSTRINAIPKDEKASIRGRLFEMID
EQNNQLCIQNAQASARIARLDFPVEWPTLFEDLENLLNDEIIRKDSVKIYNILMHINQ
IVKVLGTARIGRCRPAMQSKVPLILPLIVRIYLQSFEEWTTSSNLNYEDLSSLQVSYL
ALKVLRRIICEGYDRPQTDQSVCDFIKLSVSHFEMLISNHENFKKFDIYEKFIKCLGK
LYFNLVTGSPANFILLPCSTQILITYTRLIFDKAPKVYRENSDVTGDFWEQTAIRGLL
ILKRVINFIHKKGAITLKARSDKLTIDASINKINTEFLNENLITRLVDTLMEWYLRLR
PTELENWFMDPEEWINEQMATSYEYQIRPCAENVFQDLMNTFSELLVPYLLKKIENDA
SKLSNSLDDFLRKDAIYASFQLSASAVSEMVDFDRLLIQVFLPEATNTNISGDELRII
RRRVALIINEWSTVKCSEESKSLCYKLFTNFLTDEDDKVVLLTTVQTVRTMVDDWNFN
KDTFQPFLTENVHLLLRKILPSVSLTETRLYVLNTLSDIIIQTKPLISRDLLVEILQI
IPNLWEIATNNASEAILANALLRLLRNLVSSLGSQSHLTWDIAIPVVALACDPSSMQY
QLLSEDGYELWGMLLQNFSSHDQEFDDKFVELVPFLKYGIETHTEILPTLLEIIKSYA
LILNPVDFFSNNTFQDIFKQMSKYLLKLREDSFQLVLEIWEILILSNESDYENLLLQK
FYETGVLSALFDAIFLEEAPSSYLCSQIIQIIARISYVNPDALMTFLATYHDNLPTSN
ENARMPESIRKIVSKDQTYDSVVNKLLTGWIVCFRDIFDPKFKKVHILGISSLLRTGL
VPILTEFSSIASLWIEMLEEINETNRGDCEKYHLNDIVTEQSIAFHPLTAEQLRYHQL
CKNNDPVHNISLKDFISQSMEYLESHLGVERYQEFLKTINPSLLENLQMFLSIQPQEA
RP"
gene <316755..>317516
/gene="SPC29"
/locus_tag="YPL124W"
/gene_synonym="LPH3; NIP29"
/db_xref="GeneID:855979"
mRNA <316755..>317516
/gene="SPC29"
/locus_tag="YPL124W"
/gene_synonym="LPH3; NIP29"
/product="Spc29p"
/transcript_id="NM_001183938.1"
/db_xref="GeneID:855979"
CDS 316755..317516
/gene="SPC29"
/locus_tag="YPL124W"
/gene_synonym="LPH3; NIP29"
/experiment="EXISTENCE:direct assay:GO:0005816 spindle
pole body [PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0061493 central
plaque of mitotic spindle pole body [PMID:10339566]"
/experiment="EXISTENCE:mutant phenotype:GO:1903087 mitotic
spindle pole body duplication
[PMID:10339566|PMID:10330408]"
/note="Inner plaque spindle pole body (SPB) component;
links the central plaque component Spc42p to the inner
plaque component Spc110p; required for SPB duplication"
/codon_start=1
/product="Spc29p"
/protein_id="NP_015201.1"
/db_xref="GeneID:855979"
/db_xref="SGD:S000006045"
/translation="MDYSNFGNSASKKFQDDTLNRVRKEHEEALKKLREENFSSNTSE
LGNKKHYRAQERMSSPLHRLSPTGKSDDRKVKSPLDDKLRRQLREGNTRLPPPPFSSY
GMPPTNRSNLDRIRRRTSSPVRTDKFASQNVIDDQRLEIKYLERIVYDQGTVIDNLTS
RITRLESFILNSISDRGDKNFASLEHSRSFSGFPTNKTYGLQMGGLYENDMPYRRSSD
NINKEGAREDRSSQIHIENESTEDILKILSSSFHN"
gene complement(<317641..>318945)
/gene="RNY1"
/locus_tag="YPL123C"
/db_xref="GeneID:855980"
mRNA complement(<317641..>318945)
/gene="RNY1"
/locus_tag="YPL123C"
/product="ribonuclease T2"
/transcript_id="NM_001183937.1"
/db_xref="GeneID:855980"
CDS complement(317641..318945)
/gene="RNY1"
/locus_tag="YPL123C"
/EC_number="4.6.1.19"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:24102742]"
/experiment="EXISTENCE:direct assay:GO:0004521 RNA
endonuclease activity [PMID:11158587]"
/experiment="EXISTENCE:direct assay:GO:0005576
extracellular region [PMID:11158587]"
/experiment="EXISTENCE:direct assay:GO:0005773 vacuole
[PMID:19332891]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:19332891|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:40294649]"
/experiment="EXISTENCE:genetic interaction:GO:0006915
apoptotic process [PMID:19332891]"
/experiment="EXISTENCE:mutant phenotype:GO:0000902 cell
morphogenesis [PMID:11158587]"
/experiment="EXISTENCE:mutant phenotype:GO:0004521 RNA
endonuclease activity [PMID:19332891]"
/experiment="EXISTENCE:mutant phenotype:GO:0006401 RNA
catabolic process [PMID:19332891]"
/experiment="EXISTENCE:mutant phenotype:GO:0006402 mRNA
catabolic process [PMID:33875662]"
/note="Vacuolar RNase of the T(2) family; relocalizes to
the cytosol where it cleaves tRNAs upon oxidative or
stationary phase stress; required for tRNA-specific
translational pausing during oxidative stress; promotes
apoptosis under stress conditions and this function is
independent of Rny1p catalytic activity"
/codon_start=1
/product="ribonuclease T2"
/protein_id="NP_015202.1"
/db_xref="GeneID:855980"
/db_xref="SGD:S000006044"
/translation="MLLKNLHSLLQLPIFSNGADKGIEPNCPINIPLSCSNKTDIDNS
CCFEYPGGIFLQTQFWNYFPSKNDLNETELVKELGPLDSFTIHGLWPDNCHGGYQQFC
NRSLQIDDVYYLLHDKKFNNNDTSLQISGEKLLEYLDLYWKSNNGNHESLWIHEFNKH
GTCISTIRPECYTEWGANSVDRKRAVYDYFRITYNLFKKLDTFSTLEKNNIVPSVDNS
YSLEQIEAALSKEFEGKKVFIGCDRHNSLNEVWYYNHLKGSLLSEMFVPMDSLAIRTN
CKKDGIKFFPKGYVPTFRRRPNKGARYRGVVRLSNINNGDQMQGFLIKNGHWMSQGTP
ANYELIKSPYGNYYLRTNQGFCDIISSSSNELVCKFRNIKDAGQFDFDPTKGGDGYIG
YSGNYNWGGDTYPRRRNQSPIFSVDDEQNSKKYKFKLKFIKN"
gene complement(<319226..>320767)
/gene="TFB2"
/locus_tag="YPL122C"
/db_xref="GeneID:855981"
mRNA complement(<319226..>320767)
/gene="TFB2"
/locus_tag="YPL122C"
/product="TFIIH/NER complex subunit TFB2"
/transcript_id="NM_001183936.1"
/db_xref="GeneID:855981"
CDS complement(319226..320767)
/gene="TFB2"
/locus_tag="YPL122C"
/experiment="EXISTENCE:direct assay:GO:0000439
transcription factor TFIIH core complex
[PMID:19818408|PMID:14500720]"
/experiment="EXISTENCE:direct assay:GO:0003690
double-stranded DNA binding [PMID:19897425]"
/experiment="EXISTENCE:direct assay:GO:0005675
transcription factor TFIIH holo complex
[PMID:27381459|PMID:19818408]"
/experiment="EXISTENCE:direct assay:GO:0006366
transcription by RNA polymerase II [PMID:19818408]"
/experiment="EXISTENCE:mutant phenotype:GO:0006289
nucleotide-excision repair [PMID:9235928]"
/experiment="EXISTENCE:physical interaction:GO:0000112
nucleotide-excision repair factor 3 complex
[PMID:10681587]"
/note="Subunit of TFIIH and nucleotide excision repair
factor 3 complexes; involved in transcription initiation,
required for nucleotide excision repair, similar to 52 kDa
subunit of human TFIIH"
/codon_start=1
/product="TFIIH/NER complex subunit TFB2"
/protein_id="NP_015203.1"
/db_xref="GeneID:855981"
/db_xref="SGD:S000006043"
/translation="MSDYSLKHSVTQYLEEIPQQVQNRLYTSPATCLAIYRILPPLAK
FFIMAMVFNENEVPLLDLDKWVNSNGKLQFQNAIKSMKSLHLLIPNKSSGTLMINLNP
TFKISLRNALTGGEVQNSFGVVVEENVVSLDLLDEYSANKWETILHFMVGTPLAKIPS
EKVLNLLKHSKLMEEVNSTGEFKITNEGFQFLLQEINSQLWTLLLQYLKMIETSKMDL
VDVLHFIFMLGALEVGKAYKIDALSETQRIMLQDMRDYGLVFQKHSNDSIFYPTKLAL
MLTSDTKTIRSASNAMDSVLRQNREEPSVNEDGANGKSTTDITTSDDLNKAGLKNQDI
PDGSLIVETNFKIYSYSNSPLQIAVLSLFVHLKARFVNMVLGQITRESIRRALTNGIT
ADQIIAYLETHAHPQMRRLAEEKLEKKLELDPNCKEPLQVLPPTVVDQIRLWQLELDR
VITYEGSLYSDFETSQEYNLLSKYAQDIGVLLWKDDKKKKFFISKEGNSQVLDFAKRK
LKKKQ"
gene complement(<320962..>321630)
/gene="MEI5"
/locus_tag="YPL121C"
/gene_synonym="LPH6"
/db_xref="GeneID:855982"
mRNA complement(<320962..>321630)
/gene="MEI5"
/locus_tag="YPL121C"
/gene_synonym="LPH6"
/product="Mei5p"
/transcript_id="NM_001183935.1"
/db_xref="GeneID:855982"
CDS complement(320962..321630)
/gene="MEI5"
/locus_tag="YPL121C"
/gene_synonym="LPH6"
/experiment="EXISTENCE:direct assay:GO:0000794 condensed
nuclear chromosome [PMID:15620352|PMID:15579681]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0030234 enzyme
regulator activity [PMID:39275989]"
/experiment="EXISTENCE:mutant phenotype:GO:0000707 meiotic
DNA recombinase assembly [PMID:15620352|PMID:15579681]"
/experiment="EXISTENCE:mutant phenotype:GO:0007131
reciprocal meiotic recombination
[PMID:15620352|PMID:15579681]"
/note="Meiosis-specific protein involved in meiotic
recombination; involved in DMC1-dependent meiotic
recombination; forms heterodimer with Sae3p; proposed to
be an assembly factor for Dmc1p"
/codon_start=1
/product="Mei5p"
/protein_id="NP_015204.1"
/db_xref="GeneID:855982"
/db_xref="SGD:S000006042"
/translation="MHNQEEWLDKDKTLVNEEENTCINHSYTKKDTNNYRVGKSGIKD
LKKPTNQKEIAIKNRELTKQLTLLRQENNHLQQACKILSENKIIENRKSIEKWRTICE
MELSFILNSTLIKINRMGGYKDFLEKEMEAKKRRLEYQIDNGMEDQICEIKESDDFRQ
LSEVEKQEWESQMNEQLKELEKKKIAELEKLNKVLHDSEGKDFGMAELCTRLKLDYSL
IFPQ"
gene <322071..>323744
/gene="VPS30"
/locus_tag="YPL120W"
/gene_synonym="APG6; ATG6; VPT30"
/db_xref="GeneID:855983"
mRNA <322071..>323744
/gene="VPS30"
/locus_tag="YPL120W"
/gene_synonym="APG6; ATG6; VPT30"
/product="beclin 1"
/transcript_id="NM_001183934.1"
/db_xref="GeneID:855983"
CDS 322071..323744
/gene="VPS30"
/locus_tag="YPL120W"
/gene_synonym="APG6; ATG6; VPT30"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:19001347]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:9105038]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0034271
phosphatidylinositol 3-kinase complex, class III, type I
[PMID:11157979]"
/experiment="EXISTENCE:direct assay:GO:0034272
phosphatidylinositol 3-kinase complex, class III, type II
[PMID:11157979]"
/experiment="EXISTENCE:direct assay:GO:0120095
vacuole-isolation membrane contact site [PMID:23549786]"
/experiment="EXISTENCE:genetic interaction:GO:0042147
retrograde transport, endosome to Golgi [PMID:12244127]"
/experiment="EXISTENCE:mutant phenotype:GO:0000425
pexophagy [PMID:21121900]"
/experiment="EXISTENCE:mutant phenotype:GO:0006661
phosphatidylinositol biosynthetic process [PMID:12244127]"
/experiment="EXISTENCE:mutant phenotype:GO:0006914
autophagy [PMID:22437838]"
/experiment="EXISTENCE:mutant phenotype:GO:0016236
macroautophagy [PMID:11157979|PMID:32788210]"
/experiment="EXISTENCE:mutant phenotype:GO:0032258
cytoplasm to vacuole targeting by the Cvt pathway
[PMID:9712845|PMID:9105038]"
/experiment="EXISTENCE:mutant phenotype:GO:0034727
piecemeal microautophagy of the nucleus [PMID:18701704]"
/experiment="EXISTENCE:mutant phenotype:GO:0042147
retrograde transport, endosome to Golgi [PMID:12244127]"
/experiment="EXISTENCE:mutant phenotype:GO:0045324 late
endosome to vacuole transport [PMID:11157979]"
/experiment="EXISTENCE:mutant phenotype:GO:0051365
cellular response to potassium ion starvation
[PMID:32788210]"
/note="Subunit of phosphatidylinositol (PtdIns) 3-kinase
complexes I and II; Complex I is essential in autophagy,
Complex II is required for vacuolar protein sorting;
required for overflow degradation of misfolded proteins
when ERAD is saturated; C-terminus has novel globular fold
essential for autophagy through the targeting of the
PI3-kinase complex I to the pre-autophagosomal structure;
ortholog of higher eukaryote gene Beclin 1; human BECN1
can complement yeast null mutant"
/codon_start=1
/product="beclin 1"
/protein_id="NP_015205.1"
/db_xref="GeneID:855983"
/db_xref="SGD:S000006041"
/translation="MKCQTCHLPLQLDPSLEGLSLTQRNLLLSNNSIITATNENVISN
KGIEAADNCGPQIPKERLRRLGEIQNIKDLNLKDDKLITDSFVFLNHDDDDNANITSN
SREDQRYGNANGNDNKKANSDTSDGTSTFRDHDEEEQEATDEDENQQIQLNSKTLSTQ
VNAMTNVFNILSSQTNIDFPICQDCCNILINRLKSEYDDAIKERDTYAQFLSKLESQN
KEISESNKEKQYSHNLSEKENLKKEEERLLDQLLRLEMTDDDLDGELVRLQEKKVQLE
NEKLQKLSDQNLMDLNNIQFNKNLQSLKLQYELSLNQLDKLRKINIFNATFKISHSGP
FATINGLRLGSIPESVVPWKEINAALGQLILLLATINKNLKINLVDYELQPMGSFSKI
KKRMVNSVEYNNSTTNAPGDWLILPVYYDENFNLGRIFRKETKFDKSLETTLEIISEI
TRQLSTIASSYSSQTLTTSQDESSMNNANDVENSTSILELPYIMNKDKINGLSVKLHG
SSPNLEWTTAMKFLLTNVKWLLAFSSNLLSKSITLSPTVNYNDKTISGN"
gene complement(<324024..>324287)
/locus_tag="YPL119C-A"
/db_xref="GeneID:1466520"
mRNA complement(<324024..>324287)
/locus_tag="YPL119C-A"
/product="uncharacterized protein"
/transcript_id="NM_001184670.1"
/db_xref="GeneID:1466520"
CDS complement(324024..324287)
/locus_tag="YPL119C-A"
/note="hypothetical protein; identified by expression
profiling and mass spectrometry"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878181.1"
/db_xref="GeneID:1466520"
/db_xref="SGD:S000028859"
/translation="MHPLVDELTLSRYLTHGTSVLSSSLYSVAFFLFFFPNFLFFCSC
PNHKWVSLPFIGMDILEALCFYREGKIRNIFEIGGLLLQSFYN"
gene complement(<324411..>326264)
/gene="DBP1"
/locus_tag="YPL119C"
/gene_synonym="LPH8"
/db_xref="GeneID:855984"
mRNA complement(<324411..>326264)
/gene="DBP1"
/locus_tag="YPL119C"
/gene_synonym="LPH8"
/product="putative DEAD-box ATP-dependent RNA helicase
DBP1"
/transcript_id="NM_001183933.1"
/db_xref="GeneID:855984"
CDS complement(324411..326264)
/gene="DBP1"
/locus_tag="YPL119C"
/gene_synonym="LPH8"
/EC_number="3.6.4.13"
/experiment="EXISTENCE:direct assay:GO:0003727
single-stranded RNA binding [PMID:31299079]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:23222640|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:31299079]"
/experiment="EXISTENCE:direct assay:GO:0033677 DNA/RNA
helicase activity [PMID:21884706]"
/experiment="EXISTENCE:direct assay:GO:0051880
G-quadruplex DNA binding [PMID:30855040]"
/experiment="EXISTENCE:direct assay:GO:1901195 positive
regulation of formation of translation preinitiation
complex [PMID:31299079]"
/experiment="EXISTENCE:genetic interaction:GO:0006413
translational initiation [PMID:31299079]"
/experiment="EXISTENCE:mutant phenotype:GO:0006413
translational initiation [PMID:14763975]"
/note="ATP-dependent RNA helicase of the DEAD-box protein
family; accelerates eIF4F-dependent assembly of 48S
translation preinitiation complex (PIC), stimulating
recruitment of mRNAs with long, structured 5'-UTRs;
cooperates with DEAD-box RNA helicase and paralog Ded1p,
stimulating PIC attachment and highly processive scanning
by the 40S ribosomal subunit; binds G-quadruplex (G4) DNA;
protein abundance increases in response to DNA replication
stress"
/codon_start=1
/product="putative DEAD-box ATP-dependent RNA helicase
DBP1"
/protein_id="NP_015206.1"
/db_xref="GeneID:855984"
/db_xref="SGD:S000006040"
/translation="MADLPQKVSNLSINNKENGGGGGKSSYVPPHLRSRGKPSFERST
PKQEDKVTGGDFFRRAGRQTGNNGGFFGFSKERNGGTSANYNRGGSSNYKSSGNRWVN
GKHIPGPKNAKLEAELFGVHDDPDYHSSGIKFDNYDNIPVDASGKDVPEPILDFSSPP
LDELLMENIKLASFTKPTPVQKYSIPIVTKGRDLMACAQTGSGKTGGFLFPLFTELFR
SGPSPVPEKAQSFYSRKGYPSALVLAPTRELATQIFEEARKFTYRSWVRPCVVYGGAP
IGNQMREVDRGCDLLVATPGRLNDLLERGKVSLANIKYLVLDEADRMLDMGFEPQIRH
IVEECDMPSVENRQTLMFSATFPVDIQHLARDFLDNYIFLSVGRVGSTSENITQRILY
VDDMDKKSALLDLLSAEHKGLTLIFVETKRMADQLTDFLIMQNFKATAIHGDRTQAER
ERALSAFKANVADILVATAVAARGLDIPNVTHVINYDLPSDIDDYVHRIGRTGRAGNT
GVATSFFNSNNQNIVKGLMEILNEANQEVPTFLSDLSRQNSRGGRTRGGGGFFNSRNN
GSRDYRKHGGNGSFGSTRPRNTGTSNWGSIGGGFRNDNEKNGYGNSNASWW"
gene <326628..>327662
/gene="MRP51"
/locus_tag="YPL118W"
/gene_synonym="bS1m"
/db_xref="GeneID:855985"
mRNA <326628..>327662
/gene="MRP51"
/locus_tag="YPL118W"
/gene_synonym="bS1m"
/product="mitochondrial 37S ribosomal protein bS1m MRP51"
/transcript_id="NM_001183932.1"
/db_xref="GeneID:855985"
CDS 326628..327662
/gene="MRP51"
/locus_tag="YPL118W"
/gene_synonym="bS1m"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:9528754|PMID:11278769]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005763
mitochondrial small ribosomal subunit
[PMID:9528754|PMID:11278769]"
/experiment="EXISTENCE:genetic interaction:GO:0070124
mitochondrial translational initiation [PMID:9528754]"
/experiment="EXISTENCE:mutant phenotype:GO:0032543
mitochondrial translation [PMID:9528754]"
/note="Mitochondrial ribosomal protein of the small
subunit; MRP51 exhibits genetic interactions with
mutations in the COX2 and COX3 mRNA 5'-untranslated leader
sequences"
/codon_start=1
/product="mitochondrial 37S ribosomal protein bS1m MRP51"
/protein_id="NP_015207.1"
/db_xref="GeneID:855985"
/db_xref="SGD:S000006039"
/translation="MTLAELLGRSRIAQVANNHKPLTYTGKKFHPTHQIIETKPSTLY
RQEWGLKSAIPSKIKSRYLVYNDLDTLERITTFEPRGGTQWNRLRFQEMGVPIVSNIG
RQNPFFKYISRPEDESHAKLSLFKEMKGDTDISPAAMKKRLKKITALIRSFQDEFKEW
LVENHPDELKLNSNKLEDYVVKFLNKKLETKTNKKFNTEIIGTGGLSYSLPGKLKNSP
NGVIQRTVVPGRILNVVKENNDNKWLAAIGGFVADVVFFQSPPSSFNSMGDFIRMKTF
LFEILEASMEKNGSVSMHARLLEPQNDKTREFFNKRPIYKPLTSRRARRPSVGNIQEA
NNLLNIIKGN"
gene complement(<327864..>328730)
/gene="IDI1"
/locus_tag="YPL117C"
/gene_synonym="BOT2; LPH10"
/db_xref="GeneID:855986"
mRNA complement(<327864..>328730)
/gene="IDI1"
/locus_tag="YPL117C"
/gene_synonym="BOT2; LPH10"
/product="isopentenyl-diphosphate delta-isomerase IDI1"
/transcript_id="NM_001183931.1"
/db_xref="GeneID:855986"
CDS complement(327864..328730)
/gene="IDI1"
/locus_tag="YPL117C"
/gene_synonym="BOT2; LPH10"
/EC_number="5.3.3.2"
/experiment="EXISTENCE:direct assay:GO:0004452
isopentenyl-diphosphate delta-isomerase activity
[PMID:2681212]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0006696 ergosterol
biosynthetic process [PMID:2681212]"
/experiment="EXISTENCE:direct assay:GO:0045337 farnesyl
diphosphate biosynthetic process [PMID:7858969]"
/note="Isopentenyl diphosphate:dimethylallyl diphosphate
isomerase; catalyzes an essential activation step in the
isoprenoid biosynthetic pathway; required for viability;
isopentenyl diphosphate:dimethylallyl diphosphate
isomerase is also known as IPP isomerase"
/codon_start=1
/product="isopentenyl-diphosphate delta-isomerase IDI1"
/protein_id="NP_015208.1"
/db_xref="GeneID:855986"
/db_xref="SGD:S000006038"
/translation="MTADNNSMPHGAVSSYAKLVQNQTPEDILEEFPEIIPLQQRPNT
RSSETSNDESGETCFSGHDEEQIKLMNENCIVLDWDDNAIGAGTKKVCHLMENIEKGL
LHRAFSVFIFNEQGELLLQQRATEKITFPDLWTNTCCSHPLCIDDELGLKGKLDDKIK
GAITAAVRKLDHELGIPEDETKTRGKFHFLNRIHYMAPSNEPWGEHEIDYILFYKINA
KENLTVNPNVNEVRDFKWVSPNDLKTMFADPSYKFTPWFKIICENYLFNWWEQLDDLS
EVENDRQIHRML"
gene <329606..>331699
/gene="HOS3"
/locus_tag="YPL116W"
/db_xref="GeneID:855987"
mRNA <329606..>331699
/gene="HOS3"
/locus_tag="YPL116W"
/product="histone deacetylase"
/transcript_id="NM_001183930.1"
/db_xref="GeneID:855987"
CDS 329606..331699
/gene="HOS3"
/locus_tag="YPL116W"
/EC_number="3.5.1.98"
/experiment="EXISTENCE:direct assay:GO:0004407 histone
deacetylase activity [PMID:10535926]"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0000082 G1/S
transition of mitotic cell cycle [PMID:19823668]"
/experiment="EXISTENCE:genetic interaction:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:19823668]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:17706600]"
/experiment="EXISTENCE:physical interaction:GO:0000082
G1/S transition of mitotic cell cycle [PMID:19823668]"
/experiment="EXISTENCE:physical interaction:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:19823668]"
/note="Trichostatin A-insensitive homodimeric histone
deacetylase (HDAC); specificity in vitro for histones H3,
H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and
Hos2p; deletion results in increased histone acetylation
at rDNA repeats"
/codon_start=1
/product="histone deacetylase"
/protein_id="NP_015209.1"
/db_xref="GeneID:855987"
/db_xref="SGD:S000006037"
/translation="MSSKHSDPLERFYKQFQAFVQNNPNVISAARAAAQIPESAKAVV
VLSPYSLQHVFPREWVTKSYRKTIVERPERLLASSMGISAAITMYPSLFTLKSSHQRK
GSLMAPHVLKVHGSSWPAELIELCQMADAKLLKGEIEVPDTWNSGDIYLSSKTIKALQ
GTIGAIETGVDSIFKGPSAEHISNRAFVAIRPPGHHCHYGTPSGFCLLNNAHVAIEYA
YDTYNVTHVVVLDFDLHHGDGTQDICWKRAGFKPEEEPEDSSYDDFGKKFAEFPKVGY
FSMHDINSFPTESGFATKENIKNASTCIMNSHDLNIWNIHLSKWTTEEEFNVLYRTKY
RTLFAKADEFFRSAKLEMNQQGRPFKGLVVISAGFDASEFEQTSMQRHSVNVPTSFYT
TFTKDALKLAQMHCHGKVLSLMEGGYSDKAICSGVFAHLIGLQNQDWVKEWGSEQVVK
EIVRGCKPAWKPYKTKRAKDVIRIWAEEVIRLGRAMIPEFDDIIFKDAVNSAPSNSLL
KATVEPASTSTIAQRIIRSHRSNASPEKELHENKPRSTEKQEQREIRSDTKVKQLSSN
NRAAETQIPFLQQEFSSEDEDEEYVYDEELNKTFNRTVEDITIDDISRHLETLEIEKK
GDEDSDHELKEKNWKNSHQRRLQGNGMYKIPSNTKPHRIRQPQNANTPTYDDSDISMI
SHVSRKHTTRSGGRW"
rep_origin 331699..332100
/note="ARS1617; Replication origin; identified in multiple
array studies, confirmed by plasmid-based recombinational
ARS assay"
/db_xref="SGD:S000130199"
gene complement(<332100..>335486)
/gene="BEM3"
/locus_tag="YPL115C"
/db_xref="GeneID:855988"
mRNA complement(<332100..>335486)
/gene="BEM3"
/locus_tag="YPL115C"
/product="GTPase-activating protein BEM3"
/transcript_id="NM_001183929.1"
/db_xref="GeneID:855988"
CDS complement(332100..335486)
/gene="BEM3"
/locus_tag="YPL115C"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:17914457]"
/experiment="EXISTENCE:direct assay:GO:0005096 GTPase
activator activity
[PMID:12455995|PMID:7498791|PMID:8300560|PMID:8227021]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:17914457]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:17914457]"
/experiment="EXISTENCE:direct assay:GO:0005938 cell cortex
[PMID:17914457]"
/experiment="EXISTENCE:direct assay:GO:0032266
phosphatidylinositol-3-phosphate binding [PMID:11557775]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:17914457|PMID:19053807]"
/experiment="EXISTENCE:genetic interaction:GO:0005096
GTPase activator activity [PMID:7498791]"
/experiment="EXISTENCE:genetic interaction:GO:0031106
septin ring organization [PMID:14517318]"
/experiment="EXISTENCE:genetic interaction:GO:0035024
negative regulation of Rho protein signal transduction
[PMID:17914457]"
/experiment="EXISTENCE:mutant phenotype:GO:0030010
establishment of cell polarity [PMID:8013906]"
/experiment="EXISTENCE:mutant phenotype:GO:0035024
negative regulation of Rho protein signal transduction
[PMID:17914457]"
/experiment="EXISTENCE:physical interaction:GO:0035024
negative regulation of Rho protein signal transduction
[PMID:17914457]"
/note="Rho GTPase activating protein (RhoGAP); involved in
control of the cytoskeleton organization; targets the
essential Rho-GTPase Cdc42p, which controls establishment
and maintenance of cell polarity, including bud-site
assembly"
/codon_start=1
/product="GTPase-activating protein BEM3"
/protein_id="NP_015210.1"
/db_xref="GeneID:855988"
/db_xref="SGD:S000006036"
/translation="MTDNLTTTHGGSTTLELLAQYNDHRSKKDKSIEHIEKGTCSGKE
RNPSYDEIFTENIKLKLQVQEYETEIESLEKVIDMLQKNREASLEVVLEQVQNDSRDS
YVNDQSFVLPPRSAERKAHIKSLNLPIPTLSPPLQQGSDVALETSVTPTVPQIGVTSN
TSISRKHLQNMILNDEIEANSSFSSPKIINRSVSSPTKIHSEQLASPAASVTYTTSRI
TIKSPNKGSKSPLQERLRSPQNPNRMTAVINNHLHSPLKASTSNNLDELTESKSQQLT
NDAIQKNDRVYSSITSSAYTTGTPTSAAKSPSSLLEVKEGENKALGFSPASKEKLDDF
TQLLDSSFGEEDLVNTDSKDPLSIKSTINESLPPPPAPPTFFSPTSSGNIKNSTPLSS
HLASPVILNKKDDNFGAQSAKNLKKPVLTSSLPNLSTKLSTTSQNASLPPNPPVESSS
KQKQLGETASIHSTNTLNTFSSTPQGSLKTLRRPHASSVSTVKSVAQSLKSDIPLFVQ
PEDFGTIQIEVLSTLYRDNEDDLSILIAIIDRKSGKEMFKFSKSIHKVRELDVYMKSH
VPDLPLPTLPDRQLFQTLSPTKVDTRKNILNQYYTSIFSVPEFPKNVGLKIAQFISTD
TVMTPPMMDDNVKDGSLLLRRPKTLTGNSTWRVRYGILRDDVLQLFDKNQLTETIKLR
QSSIELIPNLPEDRFGTRNGFLITEHKKSGLSTSTKYYICTETSKERELWLSAFSDYI
DPSQSLSLSSSRNANDTDSASHLSAGTHHSKFGNATISATDTPSYVTDLTQEYNNNNN
ISNSSNNIANSDGIDSNPSSHSNFLASSSGNAEEEKDSRRAKMRSLFPFKKLTGPASA
MNHIGITISNDSDSPTSPDSIIKSPSKKLMEVSSSSNSSTGPHVSTAIFGSSLETCLR
LSSHKYQNVYDLPSVVYRCLEYLYKNRGIQEEGIFRLSGSSTVIKTLQERFDKEYDVD
LCRYNESIEAKDDEASPSLYIGVNTVSGLLKLYLRKLPHLLFGDEQFLSFKRVVDENH
NNPVQISLGFKELIESGLVPHANLSLMYALFELLVRINENSKFNKMNLRNLCIVFSPT
LNIPISMLQPFITDFACIFQGGEPVKEEEREKVDIHIPQV"
gene complement(<335953..>337143)
/locus_tag="YPL113C"
/db_xref="GeneID:855990"
mRNA complement(<335953..>337143)
/locus_tag="YPL113C"
/product="glyoxylate reductase"
/transcript_id="NM_001183927.1"
/db_xref="GeneID:855990"
CDS complement(335953..337143)
/locus_tag="YPL113C"
/experiment="EXISTENCE:mutant phenotype:GO:0016616
oxidoreductase activity, acting on the CH-OH group of
donors, NAD or NADP as acceptor [PMID:17173333]"
/note="Glyoxylate reductase; acts on glyoxylate and
hydroxypyruvate substrates; YPL113C is not an essential
gene"
/codon_start=1
/product="glyoxylate reductase"
/protein_id="NP_015212.1"
/db_xref="GeneID:855990"
/db_xref="SGD:S000006034"
/translation="MITSIDIADVTYSAKPRILVPYKTQWEVASHLPEYRKLAERVEF
YKYEMSTKDDFVKFLETHRINGFWLTEEFFTVLGNPSSYIEFFPASLKVILVPWVGCD
FIDGKLLRSKGITLCNIGPHAADHVTELAIFLAISCFRMTSFWEYCFKYVENGNVEQC
KKYISSDSYEIVTDSYHGQEMKFPSRTDKCKPNKDRKVVHLAEKYTVGGKKMESPMNK
KVLILGFGSIGQNIGSNLHKVFNMSIEYYKRTGPVQKSLLDYNAKYHSDLDDPNTWKN
ADLIILALPSTASTNNIINRKSLAWCKDGVRIVNVGRGTCIDEDVLLDALESGKVASC
GLDVFKNEETRVKQELLRRWDVTALPHIGSTVADMVIKQTLITLENVQDIFVEGGDGK
YVLN"
gene complement(<337436..>338620)
/gene="PEX25"
/locus_tag="YPL112C"
/db_xref="GeneID:855991"
mRNA complement(<337436..>338620)
/gene="PEX25"
/locus_tag="YPL112C"
/product="Pex25p"
/transcript_id="NM_001183926.1"
/db_xref="GeneID:855991"
CDS complement(337436..338620)
/gene="PEX25"
/locus_tag="YPL112C"
/experiment="EXISTENCE:direct assay:GO:0005778 peroxisomal
membrane [PMID:12135984]"
/experiment="EXISTENCE:mutant phenotype:GO:0007031
peroxisome organization [PMID:12135984]"
/experiment="EXISTENCE:mutant phenotype:GO:0016558 protein
import into peroxisome matrix [PMID:12135984]"
/note="Peripheral peroxisomal membrane peroxin; required
for the regulation of peroxisome size and maintenance,
recruits GTPase Rho1p to peroxisomes, induced by oleate,
interacts with Pex27p; PEX25 has a paralog, PEX27, that
arose from the whole genome duplication"
/codon_start=1
/product="Pex25p"
/protein_id="NP_015213.1"
/db_xref="GeneID:855991"
/db_xref="SGD:S000006033"
/translation="MSQFGTTDIVSGSETPPYSGASYQDAQDDNTHPHSSDAGAEKFS
AGSGSESHTESSRSDDEDSQAKTKMVDNITILKYILDSLSGRDKLAKIIKYALDILKL
FIEKSKRNLTVLDPSVLTYYTKILKNLTVKVALRHPITVIKVLLLSLLRNFDKKIDFI
SQQLSTFRYILRFGGTPFRVCSFLGKFNKTRKCNFQIDQIKKIWFNEASLREFLDLYY
GIFDELDLLYKLKIWTNKSFYSFVSRQESLAWQYDILLSLKDHWLNLQSLQKRQLELE
VQLKVQNNALLLSPILMHQAHKDDGSQSPIRKQLLNDLNVNNDAEVLIHKQLKAIKDE
KTLVYLDIARLSFDCMANTSDILNLKTPKGTYAVLSLGSGLTGLVKLWITTKRSLCSS
KD"
gene complement(338848..338919)
/gene="IMT2"
/locus_tag="YNCP0006C"
/db_xref="GeneID:855992"
tRNA complement(338848..338919)
/gene="IMT2"
/locus_tag="YNCP0006C"
/product="tRNA-Met"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006413
translational initiation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0016282 eukaryotic
43S preinitiation complex [PMID:12008673]"
/experiment="EXISTENCE:direct assay:GO:0033290 eukaryotic
48S preinitiation complex [PMID:17242201]"
/experiment="EXISTENCE:direct assay:GO:0071074 eukaryotic
initiation factor eIF2 binding [PMID:14698289]"
/note="Methionine initiator tRNA (tRNA-Met); predicted by
tRNAscan-SE analysis; one of four initiator methionine
tRNAs in yeast that are functional for translation"
/db_xref="GeneID:855992"
/db_xref="SGD:S000006667"
gene <339944..>340945
/gene="CAR1"
/locus_tag="YPL111W"
/gene_synonym="LPH15"
/db_xref="GeneID:855993"
mRNA <339944..>340945
/gene="CAR1"
/locus_tag="YPL111W"
/gene_synonym="LPH15"
/product="arginase"
/transcript_id="NM_001183925.1"
/db_xref="GeneID:855993"
CDS 339944..340945
/gene="CAR1"
/locus_tag="YPL111W"
/gene_synonym="LPH15"
/EC_number="3.5.3.1"
/experiment="EXISTENCE:direct assay:GO:0000050 urea cycle
[PMID:2404017]"
/experiment="EXISTENCE:direct assay:GO:0004053 arginase
activity [PMID:2404017]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:205532]"
/experiment="EXISTENCE:direct assay:GO:0008270 zinc ion
binding [PMID:1939179]"
/experiment="EXISTENCE:direct assay:GO:0030145 manganese
ion binding [PMID:1939179]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807]"
/experiment="EXISTENCE:direct assay:GO:0090368 regulation
of ornithine metabolic process [PMID:12679340]"
/experiment="EXISTENCE:direct assay:GO:0090369 ornithine
carbamoyltransferase inhibitor activity [PMID:12679340]"
/experiment="EXISTENCE:mutant phenotype:GO:0000050 urea
cycle [PMID:14582193]"
/experiment="EXISTENCE:mutant phenotype:GO:0004053
arginase activity [PMID:14582193]"
/experiment="EXISTENCE:mutant phenotype:GO:0090368
regulation of ornithine metabolic process [PMID:12679340]"
/experiment="EXISTENCE:mutant phenotype:GO:0090369
ornithine carbamoyltransferase inhibitor activity
[PMID:12679340]"
/note="Arginase, catabolizes arginine to ornithine and
urea; expression responds to both induction by arginine
and nitrogen catabolite repression; disruption decreases
production of carcinogen ethyl carbamate during wine
fermentation and also enhances freeze tolerance"
/codon_start=1
/product="arginase"
/protein_id="NP_015214.1"
/db_xref="GeneID:855993"
/db_xref="SGD:S000006032"
/translation="METGPHYNYYKNRELSIVLAPFSGGQGKLGVEKGPKYMLKHGLQ
TSIEDLGWSTELEPSMDEAQFVGKLKMEKDSTTGGSSVMIDGVKAKRADLVGEATKLV
YNSVSKVVQANRFPLTLGGDHSIAIGTVSAVLDKYPDAGLLWIDAHADINTIESTPSG
NLHGCPVSFLMGLNKDVPHCPESLKWVPGNLSPKKIAYIGLRDVDAGEKKILKDLGIA
AFSMYHVDKYGINAVIEMAMKAVHPETNGEGPIMCSYDVDGVDPLYIPATGTPVRGGL
TLREGLFLVERLAESGNLIALDVVECNPDLAIHDIHVSNTISAGCAIARCALGETLL"
gene complement(<341068..>344739)
/gene="GDE1"
/locus_tag="YPL110C"
/db_xref="GeneID:855994"
mRNA complement(<341068..>344739)
/gene="GDE1"
/locus_tag="YPL110C"
/product="glycerophosphocholine phosphodiesterase"
/transcript_id="NM_001183924.1"
/db_xref="GeneID:855994"
CDS complement(341068..344739)
/gene="GDE1"
/locus_tag="YPL110C"
/EC_number="3.1.4.2"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0046475
glycerophospholipid catabolic process [PMID:16141200]"
/experiment="EXISTENCE:mutant phenotype:GO:0047389
glycerophosphocholine phosphodiesterase activity
[PMID:16141200]"
/note="Glycerophosphocholine (GroPCho) phosphodiesterase;
hydrolyzes GroPCho to choline and glycerolphosphate, for
use as a phosphate source and as a precursor for
phosphocholine synthesis; may interact with ribosomes"
/codon_start=1
/product="glycerophosphocholine phosphodiesterase"
/protein_id="NP_015215.1"
/db_xref="GeneID:855994"
/db_xref="SGD:S000006031"
/translation="MKFGKTFANHRIPEWSSQYVGYKSLKKMIKEITRLQEDIYRAHN
KNSYDEGRPPTKMRDSSNSAQNYLDSPKIQKLLASFFFAVDRDIEKVDTFYNSQYAEY
KKRFERLLSSNQFNEIKSTLVVDANKEDAVAQTLLTKDTREMNMLLKGTSQASRLSYH
KDDLIEIQSILAELRKQFRNLKWYAELNKRAFGKILKKLDKKVGTNQQMSTMKTRILP
LQFANDSLITKDLSLLKTIWEQVTFRINSYERVMRSTSPNANANDNTEFFKIICVFIE
EDDSKGLIRELTNLYSELSLIPTRIMISVLNKAALSKSLACIDAILKVIPSLNDSEDI
NRRNFFHHHIIAIGKLIRKQEILSRKKKSQPSKYTNSEGEIVTDLRTLHTTLSAPAES
DSITEEEKSSACTLSYILEELPIHLRPCLFQHDNYKRTPLHYSCQYGLSEVTKLIIKL
MKEWNIWNEIPIDDVSAFGDAESLTPLHLCVLGAHPKTTEVLLQSLDPNVKLKSSSLL
HLATEWNNYPLLHVLLSSKRFDINYQDNELHETPLYLACRLNFFEAAVCLLYNGADLE
IREKLFGWTAIFVAAAEGFTDIVKLLIANNANFDIEDEGGWTPMEHAVLRGHLHIADM
VQIRDELVTHPHSQLNSGSEEKEPLNEISAGELNERNENGNGGNKGSLGKLAGPIKSY
GHRFLDNNESLILITLGSNDTRNKSPSISLSSEALAKVIGLETDCALSLVISCNDSID
KSSVILDLPLDDNVDAVDFKVPFKVDYSHTLYFDIVPTYGTRSLETHNRIDCQKNNNN
YVMARGVSMLNKSYSSVGVNRSILNGSVTVPIIANHTLEILGTLKFEYIIITPFEHPQ
LPLERTETYWKSLVSTRVIGHRGLGKNNPNKSLQIGENTVESFIMAASLGASYVEFDV
QLTKDNVPVVYHDFLVAETGVDIPMHELTLEQFLDLNNADKEHIQRGAGHSPHHVNGA
DTALQKYRGRSVDDSDVSTLRRAWDLHDNDPNGKSNNAHWSDNRMRLTKTFKKNNFKG
NARGHSIASSFVTLKELFKKIPANVGFNIECKFPMLDEAEEEELGQIMMEMNHWVDTV
LKVVFDNANGRDIIFSSFHPDICIMLSLKQPVIPILFLTEGGSEQMADLRASSLQNGI
RFAKKWNLLGIVSAAAPILKAPRLVQVVKSNGLVCVTYGVDNNDPENASIQIEAGVDA
VIVDSVLAIRRGLTKKNEK"
gene complement(<345264..>347389)
/gene="CQD1"
/locus_tag="YPL109C"
/gene_synonym="MCO76"
/db_xref="GeneID:855995"
mRNA complement(join(<345264..345444,345597..>347389))
/gene="CQD1"
/locus_tag="YPL109C"
/gene_synonym="MCO76"
/product="Cqd1p"
/transcript_id="NM_001183923.1"
/db_xref="GeneID:855995"
CDS complement(join(345264..345444,345597..347389))
/gene="CQD1"
/locus_tag="YPL109C"
/gene_synonym="MCO76"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14562095|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:37073556]"
/experiment="EXISTENCE:direct assay:GO:0044289
mitochondrial inner-outer membrane contact site
[PMID:37073556]"
/experiment="EXISTENCE:genetic interaction:GO:0007005
mitochondrion organization [PMID:37073556]"
/experiment="EXISTENCE:mutant phenotype:GO:0007005
mitochondrion organization [PMID:37073556]"
/experiment="EXISTENCE:mutant phenotype:GO:0055091
phospholipid homeostasis [PMID:37073556]"
/note="UbiB protein kinase-like family member;
mitochondrial inner membrane protein that interacts with
outer membrane proteins Por1p and Om14p to form a
MICOS-independent mitochondrial contact site; role in
mitochondrial organization; contributes to mitochondrial
lipid homeostasis; controls the cellular distribution of
coenzyme Q (CoQ); contains a potential mitochondrial
targeting sequence and predicted transmembrane domain"
/codon_start=1
/product="Cqd1p"
/protein_id="NP_015216.3"
/db_xref="GeneID:855995"
/db_xref="SGD:S000006030"
/translation="MSFLKFAYRNSWRYYSKSTRHFHKIPIRQFIIPTSIAFYLTQNS
FPKQNCLIYNDSLKPDPKGDTFEMGLYVSSENELQEKLKSFRSAKITESRNKLIRYLR
IFWFGFNDNIVEPVCTILRFLEISAIFLPLLLLYPISWFGHKLKITDTNITETRGSLI
WCQLLRKALELAGPSFIKLGQWAGSRTDIFSHALCHELGKLHSNVTAHSLSFTLEKLS
QALKVDKIEDAFDEFNRTPIGVGSIAQVYVGELSQKYIDKYDNIQIGKDGNRWCAIKI
LHPNVRSQIRRDLKIMKFCADAINWIPTMEWLSLPSEVDQFSILMNIQLDLRIEALNL
ERFNENFKNSIQVKFPKPFLPLSNRDVMFEEHVYGLSMEKFLSTKKQINDVELCKKVS
DPFVDAFLQMLILDDFVHADLHPGNVIIRFVKTNKYGTNIISSELESYRITHDLRKKI
EEDQDQDFVGKLKSVLTNYTPQICFIDTGIITELNEKNRINFIALFNALARFDGYRAG
ELMIERSRTPETAIDKEVFAFKVEKLVDKVKQRTFTLGTVSIGDLLDQMLSMVRSHHV
RMESDFVSVVVAILLLEGIGRQLDPNLDLFESSLPILREFGFKREAKSLLKDASTLSM
LKIWVGLEVRQLMHLSMKQIYDLVRTDQLCPNY"
gene <348446..>348952
/locus_tag="YPL108W"
/db_xref="GeneID:855996"
mRNA <348446..>348952
/locus_tag="YPL108W"
/product="uncharacterized protein"
/transcript_id="NM_001183922.1"
/db_xref="GeneID:855996"
CDS 348446..348952
/locus_tag="YPL108W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="Cytoplasmic hypothetical protein; non-essential
gene that is induced in a GDH1 deleted strain with altered
redox metabolism; GFP-fusion protein is induced in
response to the DNA-damaging agent MMS"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015217.1"
/db_xref="GeneID:855996"
/db_xref="SGD:S000006029"
/translation="MKEASDREEAPKMVEKNYSTGFRKAHGEKDQSVTKPISLDGRTG
EVIVRKSTGKTKIRKGQTEEEYTQQLQHYFEVEQGPVRTKVGWMDEVDPLVEIREGKY
DISNKHQRQVLSGFCHRLFYQCKYKECLDLSTYFLGLFEPFNVKNKMKRELEELEYMI
ERCRGHVL"
gene <349119..>349865
/gene="DPC25"
/locus_tag="YPL107W"
/db_xref="GeneID:855997"
mRNA <349119..>349865
/gene="DPC25"
/locus_tag="YPL107W"
/product="Dpc25p"
/transcript_id="NM_001183921.1"
/db_xref="GeneID:855997"
CDS 349119..349865
/gene="DPC25"
/locus_tag="YPL107W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095]"
/note="Putative mitochondrial hypothetical protein; green
fluorescent protein (GFP)-fusion protein localizes to
mitochondria; DPC25 is not an essential gene"
/codon_start=1
/product="Dpc25p"
/protein_id="NP_015218.1"
/db_xref="GeneID:855997"
/db_xref="SGD:S000006028"
/translation="MIRNQGWSLLYRIYPVRRFTRYSRVDMTFEGNTQDISTSVEERM
TTVFGGRLKGEPPRSTSRVLSGGTKKIAGVQVPAKPQEPDNCCMSGCVNCVWEIYSED
LRDWKHRRKEAAEKIAGTKEKWPKDWNPPLGLLHMENVPVELREKKLETDSKKAEQPH
DLSAIRSLFPKRKGPLPKSVLAAKRKNIALRHNYEQKDGGDQSVSESDADEGWEDIPV
YVKAFAEFESKKRLQKIRRQEEIKKRTALV"
gene complement(<350194..>352275)
/gene="SSE1"
/locus_tag="YPL106C"
/gene_synonym="LPG3; MSI3"
/db_xref="GeneID:855998"
mRNA complement(<350194..>352275)
/gene="SSE1"
/locus_tag="YPL106C"
/gene_synonym="LPG3; MSI3"
/product="adenyl-nucleotide exchange factor SSE1"
/transcript_id="NM_001183920.1"
/db_xref="GeneID:855998"
CDS complement(350194..352275)
/gene="SSE1"
/locus_tag="YPL106C"
/gene_synonym="LPG3; MSI3"
/experiment="EXISTENCE:direct assay:GO:0000774
adenyl-nucleotide exchange factor activity
[PMID:17154545|PMID:16688212]"
/experiment="EXISTENCE:direct assay:GO:0005524 ATP binding
[PMID:17154545|PMID:15028727]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0042026 protein
refolding [PMID:16688212]"
/experiment="EXISTENCE:direct assay:GO:0042277 peptide
binding [PMID:18539149]"
/experiment="EXISTENCE:genetic interaction:GO:0010499
proteasomal ubiquitin-independent protein catabolic
process [PMID:29507114]"
/experiment="EXISTENCE:genetic interaction:GO:0043161
proteasome-mediated ubiquitin-dependent protein catabolic
process [PMID:29507114]"
/experiment="EXISTENCE:mutant phenotype:GO:0005524 ATP
binding [PMID:15028727]"
/experiment="EXISTENCE:mutant phenotype:GO:0006457 protein
folding [PMID:10480867]"
/experiment="EXISTENCE:mutant phenotype:GO:0006914
autophagy [PMID:31962153]"
/note="ATPase component of heat shock protein Hsp90
chaperone complex; serves as nucleotide exchange factor to
load ATP onto the SSA class of cytosolic Hsp70s; plays a
role in prion propagation and determining prion variants;
binds unfolded proteins; member of Hsp110 subclass of
HSP70 proteins; deletion results in spindle elongation in
S phase; SSE1 has a paralog, SSE2, that arose from the
whole genome duplication"
/codon_start=1
/product="adenyl-nucleotide exchange factor SSE1"
/protein_id="NP_015219.1"
/db_xref="GeneID:855998"
/db_xref="SGD:S000006027"
/translation="MSTPFGLDLGNNNSVLAVARNRGIDIVVNEVSNRSTPSVVGFGP
KNRYLGETGKNKQTSNIKNTVANLKRIIGLDYHHPDFEQESKHFTSKLVELDDKKTGA
EVRFAGEKHVFSATQLAAMFIDKVKDTVKQDTKANITDVCIAVPPWYTEEQRYNIADA
ARIAGLNPVRIVNDVTAAGVSYGIFKTDLPEGEEKPRIVAFVDIGHSSYTCSIMAFKK
GQLKVLGTACDKHFGGRDFDLAITEHFADEFKTKYKIDIRENPKAYNRILTAAEKLKK
VLSANTNAPFSVESVMNDVDVSSQLSREELEELVKPLLERVTEPVTKALAQAKLSAEE
VDFVEIIGGTTRIPTLKQSISEAFGKPLSTTLNQDEAIAKGAAFICAIHSPTLRVRPF
KFEDIHPYSVSYSWDKQVEDEDHMEVFPAGSSFPSTKLITLNRTGDFSMAASYTDITQ
LPPNTPEQIANWEITGVQLPEGQDSVPVKLKLRCDPSGLHTIEEAYTIEDIEVEEPIP
LPEDAPEDAEQEFKKVTKTVKKDDLTIVAHTFGLDAKKLNELIEKENEMLAQDKLVAE
TEDRKNTLEEYIYTLRGKLEEEYAPFASDAEKTKLQGMLNKAEEWLYDEGFDSIKAKY
IAKYEELASLGNIIRGRYLAKEEEKKQAIRSKQEASQMAAMAEKLAAQRKAEAEKKEE
KKDTEGDVDMD"
gene complement(<352863..>355412)
/gene="SYH1"
/locus_tag="YPL105C"
/gene_synonym="MYR1"
/db_xref="GeneID:855999"
mRNA complement(<352863..>355412)
/gene="SYH1"
/locus_tag="YPL105C"
/gene_synonym="MYR1"
/product="Syh1p"
/transcript_id="NM_001183919.1"
/db_xref="GeneID:855999"
CDS complement(352863..355412)
/gene="SYH1"
/locus_tag="YPL105C"
/gene_synonym="MYR1"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:18327588]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:genetic interaction:GO:0051664
nuclear pore localization [PMID:18327588]"
/note="hypothetical protein that influences nuclear pore
distribution; co-purifies with ribosomes; contains a GYF
domain, which bind proline-rich sequences; deletion
extends chronological lifespan; SYH1 has a paralog, SMY2,
that arose from the whole genome duplication"
/codon_start=1
/product="Syh1p"
/protein_id="NP_015220.1"
/db_xref="GeneID:855999"
/db_xref="SGD:S000006026"
/translation="MNPINSLAFDLHSVKLADANSDTAALSNSNTPTMNNAALLQRPS
SIMDSIGVQRVPSPFVPGSNAISGASTVPFNAYDAEITGSPLQISANQENNSAFSAAS
SNLHMNASSPSVLNKPSSTFPNVAPYLYNATGPAPNVGNQPPPPGIESQWKYIDSNGN
IQGPFGTNNMSQWYQGGYFTPTLQICRLATSPEPFGVNDRFIRLGELTTLVNNYQDPF
VAFDFIVIRALNAVPLVAPTSSEKQKVESRDLIPVADVHSDDFTYEEILGLKFEDGSY
YHETQVWVPVDGRHITKVDRIPKISAYTAPLSTTSSRSNKTTSSHEEKVPSHEEASPE
EQEVFSEEGRTVSNITNEEESIVKNPTKQEEESRGSEKEQNILDQVQPEIEEVDRKDV
ISTADEPKSKDTPQMTSEEQKRFAKAELMAQKLLEEQQRQEEEKKRREEQRKLKKEKK
LKQKQKKEEEKLKKKKKEEGKLEKEKQKELLNNILTGDTETPSSENTATSITTNLAPW
ANKKPEGAVYNQISSALEDLKKENSSKKEKKPNRTQLDREQALKLQKEILSSAQIPKT
QTGSAWGIKPQQPIKVDIKGELMKDSTKINSQSKINKANNGDIKPDSTFIEEQKKLWE
QVQKKTKKFNRASSLDDFISRTPSPSSSALNSSNTSNAWTTVSSKSTTHIASTMPVAG
NQSKSYISLDTLRSSGGLSTATKTKMSDKSKQIGSSTSIPTLKARQVKPSRIPAYPGN
ASVSKRQEFLRWCRSQLKLNTGVQPDNVLEMLLSLPPGSESKEIIADTIYSYSSTMDG
RRFATDFIKKRLECEEEINDPLSWSEVLAMPEGSSEDWEFQVVGKKKGKRF"
gene <355700..>357676
/gene="MSD1"
/locus_tag="YPL104W"
/gene_synonym="LPG5"
/db_xref="GeneID:856000"
mRNA <355700..>357676
/gene="MSD1"
/locus_tag="YPL104W"
/gene_synonym="LPG5"
/product="aspartate--tRNA ligase MSD1"
/transcript_id="NM_001183918.1"
/db_xref="GeneID:856000"
CDS 355700..357676
/gene="MSD1"
/locus_tag="YPL104W"
/gene_synonym="LPG5"
/EC_number="6.1.1.12"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278|PMID:24769239]"
/experiment="EXISTENCE:genetic interaction:GO:0070146
mitochondrial aspartyl-tRNA aminoacylation
[PMID:10710420]"
/experiment="EXISTENCE:mutant phenotype:GO:0004815
aspartate-tRNA ligase activity [PMID:2668951]"
/experiment="EXISTENCE:mutant phenotype:GO:0005739
mitochondrion [PMID:2668951]"
/experiment="EXISTENCE:mutant phenotype:GO:0070146
mitochondrial aspartyl-tRNA aminoacylation [PMID:2668951]"
/note="Mitochondrial aspartyl-tRNA synthetase; required
for acylation of aspartyl-tRNA; yeast and bacterial
aspartyl-, asparaginyl-, and lysyl-tRNA synthetases
contain regions with high sequence similarity, suggesting
a common ancestral gene"
/codon_start=1
/product="aspartate--tRNA ligase MSD1"
/protein_id="NP_015221.1"
/db_xref="GeneID:856000"
/db_xref="SGD:S000006025"
/translation="MLARSRVCLQTITRRLADFPEANAIKKKFLFRKDTSTIKQLKGL
SSGQKIVLNGWIEQKPKRVGKNLIFGLLRDSNGDIIQLVDNKSLLKGFTLEDVVQAVG
ILSLKRKLSNEDADEYEVQLEDITVLNASNKKPAQMQDFKLSAIYPPEFRYLQLRNPK
YQDFLKKRSSISKEIRNSFNNFDFTEVETPMLFKATPEGAREFLVPTRTKRSDGKPSF
YALDQSPQQYKQLLMASGVNKYYQMARCFRDEDLRADRQPEFTQVDMEMAFANSEDVM
KIIEKTVSGVWSKFSKKRGLLTLDSKGTLVPAKKENGTVSIFRMTYEQAMTSYGIDKP
DLRAPDLKIINLGEFNAFSHLNKKFPVFEVIILRSAFSNMEEYKERWSFLTNNSNYNY
RVPIVLPIENDEQANSNWFENFHAIATFENPHLITKFLKLKKGDIVCGCTREPNHSIF
ENPTPLGRLRQLVLQSEHGKNIYHAVNKDVASWIVDFPLFSPVIIEDKSGKKEKLAYP
EYEKDRLCSTHHPFTMVKLKDYEKLEKTPEKCLGRHYDLVVNGVELGGGSTRIHDPRL
QDYIFEDILKIDNAYELFGHLLNAFDMGTPPHAGFAIGFDRMCAMICETESIRDVIAF
PKSITGADLVVKSPSVIPESILEPYNIKYSNSKK"
gene complement(<358000..>359406)
/gene="FMP30"
/locus_tag="YPL103C"
/db_xref="GeneID:856001"
mRNA complement(<358000..>359406)
/gene="FMP30"
/locus_tag="YPL103C"
/product="N-acetylphosphatidylethanolamine-hydrolyzing
phospholipase D"
/transcript_id="NM_001183917.1"
/db_xref="GeneID:856001"
CDS complement(358000..359406)
/gene="FMP30"
/locus_tag="YPL103C"
/EC_number="3.1.4.54"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:21306442]"
/experiment="EXISTENCE:mutant phenotype:GO:0070291
N-acylethanolamine metabolic process [PMID:15878693]"
/experiment="EXISTENCE:mutant phenotype:GO:0070292
N-acylphosphatidylethanolamine metabolic process
[PMID:15878693]"
/note="Protein with a role in maintaining mitochondrial
morphology; also involved in maintaining normal
cardiolipin levels; mitochondrial inner membrane protein;
proposed to be involved in N-acylethanolamine metabolism;
related to mammalian N-acylPE-specific phospholipase D"
/codon_start=1
/product="N-acetylphosphatidylethanolamine-hydrolyzing
phospholipase D"
/protein_id="NP_015222.1"
/db_xref="GeneID:856001"
/db_xref="SGD:S000006024"
/translation="MNFVTCHVQMRLLLQRRLVRLRESELFRPQTSLSTFKRHASQKT
RPIQKCSRKYARILLLSVLVPYTGYAFYVSLATVKQIDLRNEMCQRLEENNNEVTYKG
SLLKYSPLEVLGRFENPFEEYRIQTVFEFFANRVFELFERNRGGIPRDVHQMNKLMPV
HKPTWGPNLVDVDPAEETALPLECKVLDELHIPTAVEENEGSKCPVYNTWLGQSCNYT
VYNGLRILTDPLFSDFLIHKTLGPKRITQMPSQITEVPKPDIILVSHNHPDHLDLESL
EYWSGKDSPLWIVPKGMKSYMTSNGCDNVLELSWWETLQVKKNNEIYHISATPAMHWS
GRSLLDTNKSLWCSFLLTHHGNPILFHAGDTGYVKDLFVRIKERFGKGCKLALLPCGQ
YCPEWHQKPRHINPQEVLKIMKDLEARNVLGVHWGTFVLSGEYFLEPKEKLEMLAEWG
GFKDRCYCPELGKTECFD"
gene <360209..>361579
/gene="ELP4"
/locus_tag="YPL101W"
/gene_synonym="HAP1; KTI9; TOT7"
/db_xref="GeneID:856002"
mRNA <360209..>361579
/gene="ELP4"
/locus_tag="YPL101W"
/gene_synonym="HAP1; KTI9; TOT7"
/product="Elongator subunit ELP4"
/transcript_id="NM_001183915.1"
/db_xref="GeneID:856002"
CDS 360209..361579
/gene="ELP4"
/locus_tag="YPL101W"
/gene_synonym="HAP1; KTI9; TOT7"
/experiment="EXISTENCE:direct assay:GO:0000049 tRNA
binding [PMID:22343726]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:22343726]"
/experiment="EXISTENCE:direct assay:GO:0033588 elongator
holoenzyme complex [PMID:11689709]"
/experiment="EXISTENCE:mutant phenotype:GO:0002098 tRNA
wobble uridine modification [PMID:18755837]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:11689709]"
/note="Subunit of hexameric RecA-like ATPase Elp456
Elongator subcomplex; which is required for modification
of wobble nucleosides in tRNA; required for Elongator
structural integrity; null mutation is functionally
complemented by human ELP4"
/codon_start=1
/product="Elongator subunit ELP4"
/protein_id="NP_015224.1"
/db_xref="GeneID:856002"
/db_xref="SGD:S000006022"
/translation="MSFRKRGEILNDRGSGLRGPLLRGPPRTSSTPLRTGNRRAPGNV
PLSDTTARLKKLNIADESKTKMGLDSSHVGVRPSPATSQPTTSTGSADLDSILGHMGL
PLGNSVLVEEQSTTEFHSILGKLFAAQGIVHNRISDSSADKTRNGDTHVIVLSLNQMF
AKELPGIYKGSRKQMKKNLISEEESKVTVQNLNETQRSTPSRYKDLKIAWKYKLADEK
RLGSPDRDDIQQNSEYKDYNHQFDITTRLMPAPIASELTFIAPTQPVSTILSQIEQTI
KRNDKKLIRIVIPSLLHPAMYPPKMFESSEIIGLMHGVRSLVKKYYERVVLFASISID
IITPPLLVLLRNMFDSVINLEPFNQEMTEFLERVYKSQPGKIQHGLVHILKLPVFTDR
GEMRVLKSEWAFKNGRKKFEIEQWGIPVDDAEGSAASEQSHSHSHSDEISHNIPAKKT
KISLDY"
gene <361872..>363362
/gene="ATG21"
/locus_tag="YPL100W"
/gene_synonym="HSV1; MAI1"
/db_xref="GeneID:856004"
mRNA <361872..>363362
/gene="ATG21"
/locus_tag="YPL100W"
/gene_synonym="HSV1; MAI1"
/product="Atg21p"
/transcript_id="NM_001183914.1"
/db_xref="GeneID:856004"
CDS 361872..363362
/gene="ATG21"
/locus_tag="YPL100W"
/gene_synonym="HSV1; MAI1"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane
[PMID:11852075|PMID:15194695|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0000407 phagophore
assembly site [PMID:25691244]"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:18769150]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:11852075]"
/experiment="EXISTENCE:direct assay:GO:0032266
phosphatidylinositol-3-phosphate binding
[PMID:16876790|PMID:15155809]"
/experiment="EXISTENCE:direct assay:GO:0070273
phosphatidylinositol-4-phosphate binding [PMID:15155809]"
/experiment="EXISTENCE:direct assay:GO:0080025
phosphatidylinositol-3,5-bisphosphate binding
[PMID:15103325]"
/experiment="EXISTENCE:mutant phenotype:GO:0000407
phagophore assembly site [PMID:25691244]"
/experiment="EXISTENCE:mutant phenotype:GO:0000422
autophagy of mitochondrion [PMID:19793921]"
/experiment="EXISTENCE:mutant phenotype:GO:0006497 protein
lipidation [PMID:15155809]"
/experiment="EXISTENCE:mutant phenotype:GO:0016050 vesicle
organization [PMID:15155809]"
/experiment="EXISTENCE:mutant phenotype:GO:0016236
macroautophagy [PMID:15194695]"
/experiment="EXISTENCE:mutant phenotype:GO:0032258
cytoplasm to vacuole targeting by the Cvt pathway
[PMID:16876790]"
/experiment="EXISTENCE:mutant phenotype:GO:0034497 protein
localization to phagophore assembly site
[PMID:25691244|PMID:15155809]"
/experiment="EXISTENCE:mutant phenotype:GO:0034727
piecemeal microautophagy of the nucleus [PMID:18701704]"
/note="Phosphoinositide binding protein; required for
vesicle formation in cytoplasm-to-vacuole targeting (CVT)
pathway, autophagy, micronucleophagy and mitophagy; binds
to several phosphoinositides including PtdIns(3,5)P2, PI3P
and PI4P; involved in PI3P-dependent recruitment and
organization of both Atg12-Atg5-Atg16 complex and Atg8p at
pre-autophagosomal structure; necessary for
oxidant-induced cell death; targeted to vacuole via AP-3
pathway; WD-40 repeat containing PROPPIN family member"
/codon_start=1
/product="Atg21p"
/protein_id="NP_015225.1"
/db_xref="GeneID:856004"
/db_xref="SGD:S000006021"
/translation="MKVLQFNQDATCCVVAASSHQISIFNCDPFGKCFEIDTKNSKKK
TSNNNGSASNSESRNNEESILITNGSRDRTDAEEEEDNEDNALVTGNILKEGEFVIEM
LFSTSLIAIADRGQGLNKGKKLKIVNTKRKCTICEIVFPHEIVDVVMNRKRMCVLLES
DQIFIYDISCMKPLETIDLWEDHYKRSQANSFSNASNTGTLEGDSANLNRVATNLLAN
ATQKSVNGSNPSVRTRRNSLRSKIRPRMVLSNDDRSILCFTAYSSPKKNKPNSEALYD
VVIYDTLNVTPVNYLNSVHKGNVACLAVSHDGKLLATASDKGTIIRVFHTGVDSDYMS
SRSLFKEFRRGTRLCNLYQLAFDKSMTMIGCVGDTDTIHLFKLDDASNSLPGDNSSNG
HWNEEEYILASNSNPSMGTPKEIPLSKPRIANYFSKKIKSSIPNQNLSRNFAYITVNE
SNRSCLGFPDEFPNQVYIASDDGTFSIYSIPSKPGECVLTKNNKFT"
gene complement(<363520..>364068)
/gene="INA17"
/locus_tag="YPL099C"
/gene_synonym="AIM43; FMP14"
/db_xref="GeneID:856005"
mRNA complement(<363520..>364068)
/gene="INA17"
/locus_tag="YPL099C"
/gene_synonym="AIM43; FMP14"
/product="Ina17p"
/transcript_id="NM_001183913.1"
/db_xref="GeneID:856005"
CDS complement(363520..364068)
/gene="INA17"
/locus_tag="YPL099C"
/gene_synonym="AIM43; FMP14"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:14576278|PMID:26928762|PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:24942160]"
/experiment="EXISTENCE:direct assay:GO:1990524 INA complex
[PMID:24942160]"
/experiment="EXISTENCE:genetic interaction:GO:0033615
mitochondrial proton-transporting ATP synthase complex
assembly [PMID:24942160]"
/experiment="EXISTENCE:mutant phenotype:GO:0033615
mitochondrial proton-transporting ATP synthase complex
assembly [PMID:24942160]"
/experiment="EXISTENCE:physical interaction:GO:1990524 INA
complex [PMID:24942160]"
/note="F1F0 ATPase synthase peripheral stalk assembly
factor; subunit of the matrix-exposed inner mitochondrial
membrane localized INA complex (Ina22p-Ina17p) involved in
assembly of the F1F0 peripheral stalk; co-purifies with
Ina22p and ATP synthase subunits; null mutant displays
elevated frequency of mitochondrial genome loss and has a
respiratory growth defect"
/codon_start=1
/product="Ina17p"
/protein_id="NP_015226.1"
/db_xref="GeneID:856005"
/db_xref="SGD:S000006020"
/translation="MLKRRSNALITLSRTKLFPITTVAYYHRRLLNQQRRAVSTSPKK
EIKSLEDLANLDSLDGVDTELIRDLINEHTTKLNIKKELDMLKKFSQEEESGHEIPVK
RFIRPLWMFILMGSSVYLLLHFSWWKLEHEERESQLKKEVEILEHQLNELIVQDKTHN
TSRGKGSNESTHMKPWYRRWFW"
gene complement(<364386..>364727)
/gene="MGR2"
/locus_tag="YPL098C"
/db_xref="GeneID:856006"
mRNA complement(<364386..>364727)
/gene="MGR2"
/locus_tag="YPL098C"
/product="Mgr2p"
/transcript_id="NM_001183912.1"
/db_xref="GeneID:856006"
CDS complement(364386..364727)
/gene="MGR2"
/locus_tag="YPL098C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0030150 protein
import into mitochondrial matrix [PMID:22613836]"
/experiment="EXISTENCE:mutant phenotype:GO:0045039 protein
insertion into mitochondrial inner membrane
[PMID:25454944]"
/experiment="EXISTENCE:physical interaction:GO:0005744
TIM23 mitochondrial import inner membrane translocase
complex [PMID:22613836]"
/note="Core component of the TIM23 protein translocase
complex; couples Tim21 with the core Tim23 translocase;
provides quality control for membrane protein insertion,
regulating their release into the inner membrane by the
TIM23 complex; required for mitochondrial import of
presequence-containing proteins at elevated temperature;
forms the translocation path with Tim17p, sealing the
lateral opening of the Tim17p cavity; required for
viability of cells lacking the mitochondrial genome
(petite-negative)"
/codon_start=1
/product="Mgr2p"
/protein_id="NP_015227.1"
/db_xref="GeneID:856006"
/db_xref="SGD:S000006019"
/translation="MPPLPQNYAQQQPSNWDKFKMGLMMGTTVGVCTGILFGGFAIAT
QGPGPDGVVRTLGKYIAGSAGTFGLFMSIGSIIRSDSESSPMSHPNLNLQQQARLEMW
KLRAKYGIRKD"
gene <364952..>366430
/gene="MSY1"
/locus_tag="YPL097W"
/db_xref="GeneID:856007"
mRNA <364952..>366430
/gene="MSY1"
/locus_tag="YPL097W"
/product="tyrosine--tRNA ligase MSY1"
/transcript_id="NM_001183911.1"
/db_xref="GeneID:856007"
CDS 364952..366430
/gene="MSY1"
/locus_tag="YPL097W"
/EC_number="6.1.1.1"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:genetic interaction:GO:0004831
tyrosine-tRNA ligase activity [PMID:3315228]"
/experiment="EXISTENCE:genetic interaction:GO:0070184
mitochondrial tyrosyl-tRNA aminoacylation [PMID:3315228]"
/experiment="EXISTENCE:mutant phenotype:GO:0005739
mitochondrion [PMID:3315228]"
/note="Mitochondrial tyrosyl-tRNA synthetase"
/codon_start=1
/product="tyrosine--tRNA ligase MSY1"
/protein_id="NP_015228.1"
/db_xref="GeneID:856007"
/db_xref="SGD:S000006018"
/translation="MLELRSCSNLVNSSRRLVPLVTYSGLSAITLPKSRFYSQPSALE
VQGTSDSRSDNILDELKQRGLVSQVSQPESFLRTKLNGNDKIKLYCGVDPTAQSLHLG
NLVPLMVLLHFYVKGHDIVTVIGGATGKVGDPSGRKTERDVMENDIRQSNVASISQQL
QRFFKNGLEYYRNRCALTEDVPSGKYTPRNNFNWWKDIKMLDFLADFGRHIRVQSMLA
RDSISSRLQTKNGLGFNEFTYQVLQAYDFYHLYKEENVTIQVGGNDQWGNITAGIDLI
NRIQPIKNKGLPFGITVPLLTTATGEKFGKSAGNAVFIDPSINTAYDVYQFFYNTLDA
DVPKFLKIFTFLNSSEIKKIVETHIKSPSLRYGQTLLAKEVTDMLYGVGSGSDSEALS
NIIFGRYDGTLSAAKLVDLCKKARILQYADREIDLIKLICKLVNCSVSEARRKLSQGS
VYLHHSKSKVNENISNLAPFLIDDRVLILRIGKQKCFIIEMR"
gene complement(<366529..>366735)
/gene="ERI1"
/locus_tag="YPL096C-A"
/gene_synonym="RIN1"
/db_xref="GeneID:856008"
mRNA complement(<366529..>366735)
/gene="ERI1"
/locus_tag="YPL096C-A"
/gene_synonym="RIN1"
/product="Eri1p"
/transcript_id="NM_001184515.1"
/db_xref="GeneID:856008"
CDS complement(366529..366735)
/gene="ERI1"
/locus_tag="YPL096C-A"
/gene_synonym="RIN1"
/experiment="EXISTENCE:direct assay:GO:0005095 GTPase
inhibitor activity [PMID:12832483]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:12832483]"
/experiment="EXISTENCE:mutant phenotype:GO:0006506 GPI
anchor biosynthetic process [PMID:15163411]"
/experiment="EXISTENCE:mutant phenotype:GO:0007265 Ras
protein signal transduction [PMID:12832483]"
/experiment="EXISTENCE:physical interaction:GO:0000506
glycosylphosphatidylinositol-N-
acetylglucosaminyltransferase (GPI-GnT) complex
[PMID:15163411]"
/note="Endoplasmic reticulum membrane protein that binds
and inhibits Ras2p; binds to and inhibits GTP-bound Ras2p
at the endoplasmic reticulum (ER); component of the
GPI-GnT complex which catalyzes the first step in
GPI-anchor biosynthesis; probable homolog of mammalian
PIG-Y protein"
/codon_start=1
/product="Eri1p"
/protein_id="NP_690846.1"
/db_xref="GeneID:856008"
/db_xref="SGD:S000028423"
/translation="MRPRDQGFLVLGFTYSVLLISLATFYWLRNNDSFLHYWCVLLLC
PATLWLWALIAWCDSEMFASSKDE"
gene <366927..>368018
/gene="PNG1"
/locus_tag="YPL096W"
/db_xref="GeneID:856009"
mRNA <366927..>368018
/gene="PNG1"
/locus_tag="YPL096W"
/product="peptide-N4-(N-acetyl-beta-
glucosaminyl)asparagine amidase"
/transcript_id="NM_001183910.1"
/db_xref="GeneID:856009"
CDS 366927..368018
/gene="PNG1"
/locus_tag="YPL096W"
/EC_number="3.5.1.52"
/experiment="EXISTENCE:direct assay:GO:0000224
peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase
activity [PMID:10831608]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10831608]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:10831608|PMID:15910746]"
/experiment="EXISTENCE:direct assay:GO:0006517 protein
deglycosylation [PMID:10831608]"
/experiment="EXISTENCE:mutant phenotype:GO:0006515 protein
quality control for misfolded or incompletely synthesized
proteins [PMID:10831608]"
/experiment="EXISTENCE:mutant phenotype:GO:0006517 protein
deglycosylation [PMID:27433019|PMID:25497214]"
/experiment="EXISTENCE:mutant phenotype:GO:0036503 ERAD
pathway [PMID:25497214]"
/experiment="EXISTENCE:mutant phenotype:GO:0097466
ubiquitin-dependent glycoprotein ERAD pathway
[PMID:27433019]"
/note="Conserved peptide N-glycanase; deglycosylating
enzyme that cleaves N-glycans that are attached to
misfolded ERAD substrate glycoproteins prior to
proteasome-dependent degradation; localizes to the
cytoplasm and nucleus; activity is enhanced by interaction
with Rad23p; human ortholog NGLY1 is associated with a
syndrome characterized by developmental delays, epilepsy,
absence of tears and liver disease"
/codon_start=1
/product="peptide-N4-(N-acetyl-beta-
glucosaminyl)asparagine amidase"
/protein_id="NP_015229.1"
/db_xref="GeneID:856009"
/db_xref="SGD:S000006017"
/translation="MGEVYEKNNIDFDSIAKMLLIKYKDFILSKFKKAAPVENIRFQN
LVHTNQFAQGVLGQSQHLCTVYDNPSWHSIVLETLDLDLIYKNVDKEFAKDGHAEGEN
IYTDYLVKELLRYFKQDFFKWCNKPDCNHCGQNTSENMTPLGSQGPNGEESKFNCGTV
EIYKCNRCGNITRFPRYNDPIKLLETRKGRCGEWCNLFTLILKSFGLDVRYVWNREDH
VWCEYFSNFLNRWVHVDSCEQSFDQPYIYSINWNKKMSYCIAFGKDGVVDVSKRYILQ
NELPRDQIKEEDLKFLCQFITKRLRYSLNDDEIYQLACRDEQEQIELIRGKTQETKSE
SVSAASKSSNRGRESGSADWKAQRGEDGK"
gene complement(<368071..>369441)
/gene="EEB1"
/locus_tag="YPL095C"
/db_xref="GeneID:856010"
mRNA complement(<368071..>369441)
/gene="EEB1"
/locus_tag="YPL095C"
/product="medium-chain fatty acid ethyl ester
synthase/esterase"
/transcript_id="NM_001183909.1"
/db_xref="GeneID:856010"
CDS complement(368071..369441)
/gene="EEB1"
/locus_tag="YPL095C"
/EC_number="2.3.1.84"
/experiment="EXISTENCE:direct assay:GO:0004026 alcohol
O-acetyltransferase activity [PMID:16361250]"
/experiment="EXISTENCE:direct assay:GO:0034338 short-chain
carboxylesterase activity [PMID:16361250]"
/experiment="EXISTENCE:direct assay:GO:0051792
medium-chain fatty acid biosynthetic process
[PMID:16361250]"
/experiment="EXISTENCE:mutant phenotype:GO:0051792
medium-chain fatty acid biosynthetic process
[PMID:16361250]"
/experiment="EXISTENCE:mutant phenotype:GO:0051793
medium-chain fatty acid catabolic process [PMID:16361250]"
/note="Acyl-coenzymeA:ethanol O-acyltransferase;
responsible for the major part of medium-chain fatty acid
ethyl ester biosynthesis during fermentation; possesses
short-chain esterase activity; may be involved in lipid
metabolism and detoxification; EEB1 has a paralog, EHT1,
that arose from the whole genome duplication"
/codon_start=1
/product="medium-chain fatty acid ethyl ester
synthase/esterase"
/protein_id="NP_015230.1"
/db_xref="GeneID:856010"
/db_xref="SGD:S000006016"
/translation="MFRSGYYPTVTPSHWGYNGTVKHVLGEKGTKSLAFRDSKRQIPL
HEFVTKHVPTLKDGANFRLNSLLFTGYLQTLYLSAGDFSKKFQVFYGREIIKFSDGGV
CTADWVMPEWEQTYSLNAEKASFNEKQFSNDEKATHPKGWPRLHPRTRYLSSEELEKC
HSKGYSYPLVVVLHGLAGGSHEPLIRALSEDLSKVGDGKFQVVVLNARGCSRSKVTTR
RIFTALHTGDVREFLNHQKALFPQRKIYAVGTSFGAAMLTNYLGEEGDNCPLNAAVAL
SNPWDFVHTWDKLAHDWWSNHIFSRTLTQFLTRTVKVNMNELQVPENFEVSHKPTVEK
PVFYTYTRENLEKAEKFTDILEFDNLFTAPSMGLPDGLTYYRKASSINRLPNIKIPTL
IINATDDPVTGENVIPYKQARENPCVLLCETDLGGHLAYLDNESNSWLTKQAAEFLGS
FDELVL"
gene complement(<369839..>370663)
/gene="SEC62"
/locus_tag="YPL094C"
/gene_synonym="LPG14"
/db_xref="GeneID:856011"
mRNA complement(<369839..>370663)
/gene="SEC62"
/locus_tag="YPL094C"
/gene_synonym="LPG14"
/product="Sec63 complex subunit SEC62"
/transcript_id="NM_001183908.1"
/db_xref="GeneID:856011"
CDS complement(369839..370663)
/gene="SEC62"
/locus_tag="YPL094C"
/gene_synonym="LPG14"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762|PMID:27831485|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008320 protein
transmembrane transporter activity [PMID:9252322]"
/experiment="EXISTENCE:direct assay:GO:0031204
post-translational protein targeting to membrane,
translocation [PMID:7758110]"
/experiment="EXISTENCE:physical interaction:GO:0031207
Sec62/Sec63 complex [PMID:2000150]"
/note="Essential subunit of Sec63 complex; with Sec61
complex, Kar2p/BiP and Lhs1p forms a channel competent for
SRP-dependent and post-translational SRP-independent
protein targeting and import into the ER;
cotranslationally N-acetylated by NatA; other members are
Sec63p, Sec66p, and Sec72p"
/codon_start=1
/product="Sec63 complex subunit SEC62"
/protein_id="NP_015231.2"
/db_xref="GeneID:856011"
/db_xref="SGD:S000006015"
/translation="MSAVGPGSNAGASVNGGSATAIATLLRNHKELKQRQGLFQAKQT
DFFRYKRFVRALHSEEYANKSARQPEIYPTIPSNKIEDQLKSREIFIQLIKAQMVIPV
KKLHSQECKEHGLKPSKDFPHLIVSNKAQLEADEYFVWNYNPRTYMDYLIVIGVVSII
LALVCYPLWPRSMRRGSYYVSLGAFGILAGFFAVAILRLILYVLSLIVYKDVGGFWIF
PNLFEDCGVLESFKPLYGFGEKDTYSYKKKLKRMKKKQAKRESNKKKAINEKAEQN"
gene <370978..>372921
/gene="NOG1"
/locus_tag="YPL093W"
/db_xref="GeneID:856012"
mRNA <370978..>372921
/gene="NOG1"
/locus_tag="YPL093W"
/product="putative GTPase NOG1"
/transcript_id="NM_001183907.1"
/db_xref="GeneID:856012"
CDS 370978..372921
/gene="NOG1"
/locus_tag="YPL093W"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:11112701]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:31909713]"
/experiment="EXISTENCE:direct assay:GO:0030687
preribosome, large subunit precursor
[PMID:17443350|PMID:16861225|PMID:11583614|PMID:23212245]"
/experiment="EXISTENCE:direct assay:GO:0042273 ribosomal
large subunit biogenesis [PMID:16888624]"
/experiment="EXISTENCE:mutant phenotype:GO:0000054
ribosomal subunit export from nucleus [PMID:12773575]"
/experiment="EXISTENCE:mutant phenotype:GO:0006364 rRNA
processing [PMID:12773575]"
/experiment="EXISTENCE:mutant phenotype:GO:0042273
ribosomal large subunit biogenesis [PMID:17443350]"
/experiment="EXISTENCE:mutant phenotype:GO:1902626
assembly of large subunit precursor of preribosome
[PMID:22735702|PMID:31909713]"
/note="Putative GTPase; associates with free 60S ribosomal
subunits in the nucleolus and is required for 60S
ribosomal subunit biogenesis; constituent of 66S
pre-ribosomal particles; member of the ODN family of
nucleolar G-proteins"
/codon_start=1
/product="putative GTPase NOG1"
/protein_id="NP_015232.1"
/db_xref="GeneID:856012"
/db_xref="SGD:S000006014"
/translation="MQLSWKDIPTVAPANDLLDIVLNRTQRKTPTVIRPGFKITRIRA
FYMRKVKYTGEGFVEKFEDILKGFPNINDVHPFHRDLMDTLYEKNHYKISLAAISRAK
SLVEQVARDYVRLLKFGQSLFQCKQLKRAALGRMATIVKKLRDPLAYLEQVRQHIGRL
PSIDPNTRTLLICGYPNVGKSSFLRCITKSDVDVQPYAFTTKSLYVGHFDYKYLRFQA
IDTPGILDRPTEEMNNIEMQSIYAIAHLRSCVLYFMDLSEQCGFTIEAQVKLFHSIKP
LFANKSVMVVINKTDIIRPEDLDEERAQLLESVKEVPGVEIMTSSCQLEENVMEVRNK
ACEKLLASRIENKLKSQSRINNVLNKIHVAQPQARDDVKRTPFIPESVKNLKKYDPED
PNRRKLARDIEAENGGAGVFNVNLKDKYLLEDDEWKNDIMPEILDGKNVYDFLDPEIA
AKLQALEEEEEKLENEGFYNSDDEEEIYDGFEASEVDDIKEKAAWIRNRQKTMIAEAR
NRKSLKNKAIMPRSKLTKSFGKMEEHMSTLGHDMSALQDKQNRAARKNRYVERGSDVV
FGDQDALTASTENGVKLRQTDRLLDGVADGSMRSKADRMAKMERRERNRHAKQGESDR
HNAVSLSKHLFSGKRGVGKTDFR"
gene <373793..>375169
/gene="SSU1"
/locus_tag="YPL092W"
/gene_synonym="LPG16"
/db_xref="GeneID:856013"
mRNA <373793..>375169
/gene="SSU1"
/locus_tag="YPL092W"
/gene_synonym="LPG16"
/product="Ssu1p"
/transcript_id="NM_001183906.1"
/db_xref="GeneID:856013"
CDS 373793..375169
/gene="SSU1"
/locus_tag="YPL092W"
/gene_synonym="LPG16"
/experiment="EXISTENCE:direct assay:GO:0000316 sulfite
transmembrane transport [PMID:10870099]"
/experiment="EXISTENCE:direct assay:GO:0000319 sulfite
transmembrane transporter activity [PMID:10870099]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:9294463]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="Plasma membrane sulfite pump involved in sulfite
metabolism; required for efficient sulfite efflux; major
facilitator superfamily protein"
/codon_start=1
/product="Ssu1p"
/protein_id="NP_015233.1"
/db_xref="GeneID:856013"
/db_xref="SGD:S000006013"
/translation="MVANWVLALTRQFDPFMFMMVMGVGISSNILYSFPYPARWLRIC
SYIMFAIACLIFIAVQALQILHLIVYIKEKSFREYFNDFFRNMKHNLFWGTYPMGLVT
IINFLGALSKANTTKSPTNARNLMIFVYVLWWYDLAVCLVIAWGISFLIWHDYYPLEG
IGNYPSYNIKMASENMKSVLLLDIIPLVVVASSCGTFTMSEIFFHAFNRNIQLITLVI
CALTWLHAIIFVFILIAIYFWSLYINKIPPMTQVFTLFLLLGPMGQGSFGVLLLTDNI
KKYAGKYYPTDNITREQEILTIAVPWCFKILGMVSAMALLAMGYFFTVISVVSILSYY
NKKEIENETGKVKRVYTFHKGFWGMTFPMGTMSLGNEELYVQYNQYVPLYAFRVLGTI
YGGVCVCWSILCLLCTLHEYSKKMLHAARKSSLFSESGTEKTTVSPYNSIESVEESNS
ALDFTRLA"
gene <375502..>376953
/gene="GLR1"
/locus_tag="YPL091W"
/gene_synonym="LPG17"
/db_xref="GeneID:856014"
mRNA <375502..>376953
/gene="GLR1"
/locus_tag="YPL091W"
/gene_synonym="LPG17"
/product="glutathione-disulfide reductase GLR1"
/transcript_id="NM_001183905.1"
/db_xref="GeneID:856014"
CDS 375502..376953
/gene="GLR1"
/locus_tag="YPL091W"
/gene_synonym="LPG17"
/EC_number="1.8.1.7"
/experiment="EXISTENCE:direct assay:GO:0004362
glutathione-disulfide reductase (NADPH) activity
[PMID:19049979|PMID:7737505]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:23613772|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:16823961|PMID:14562095|PMID:14576278|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:36164978]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:23613772]"
/experiment="EXISTENCE:genetic interaction:GO:0010731
protein glutathionylation [PMID:20074363]"
/experiment="EXISTENCE:mutant phenotype:GO:0006749
glutathione metabolic process [PMID:23242256]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress [PMID:8951820]"
/experiment="EXISTENCE:mutant phenotype:GO:0045454 cell
redox homeostasis [PMID:23242256]"
/note="Cytosolic and mitochondrial glutathione
oxidoreductase; converts oxidized glutathione to reduced
glutathione; cytosolic Glr1p is the main determinant of
the glutathione redox state of the mitochondrial
intermembrane space; mitochondrial Glr1p has a role in
resistance to hyperoxia; also detected in peroxisomes;
protein abundance increases in response to DNA replication
stress"
/codon_start=1
/product="glutathione-disulfide reductase GLR1"
/protein_id="NP_015234.1"
/db_xref="GeneID:856014"
/db_xref="SGD:S000006012"
/translation="MLSATKQTFRSLQIRTMSTNTKHYDYLVIGGGSGGVASARRAAS
YGAKTLLVEAKALGGTCVNVGCVPKKVMWYASDLATRVSHANEYGLYQNLPLDKEHLT
FNWPEFKQKRDAYVHRLNGIYQKNLEKEKVDVVFGWARFNKDGNVEVQKRDNTTEVYS
ANHILVATGGKAIFPENIPGFELGTDSDGFFRLEEQPKKVVVVGAGYIGIELAGVFHG
LGSETHLVIRGETVLRKFDECIQNTITDHYVKEGINVHKLSKIVKVEKNVETDKLKIH
MNDSKSIDDVDELIWTIGRKSHLGMGSENVGIKLNSHDQIIADEYQNTNVPNIYSLGD
VVGKVELTPVAIAAGRKLSNRLFGPEKFRNDKLDYENVPSVIFSHPEAGSIGISEKEA
IEKYGKENIKVYNSKFTAMYYAMLSEKSPTRYKIVCAGPNEKVVGLHIVGDSSAEILQ
GFGVAIKMGATKADFDNCVAIHPTSAEELVTMR"
gene complement(<377291..>378395)
/gene="RPS6A"
/locus_tag="YPL090C"
/db_xref="GeneID:856015"
mRNA complement(join(<377291..377995,378390..>378395))
/gene="RPS6A"
/locus_tag="YPL090C"
/product="40S ribosomal protein eS6 RPS6A"
/transcript_id="NM_001183904.1"
/db_xref="GeneID:856015"
CDS complement(join(377291..377995,378390..378395))
/gene="RPS6A"
/locus_tag="YPL090C"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:6814480]"
/experiment="EXISTENCE:direct assay:GO:0022627 cytosolic
small ribosomal subunit [PMID:6814480]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/experiment="EXISTENCE:direct assay:GO:0032040
small-subunit processome [PMID:15590835]"
/experiment="EXISTENCE:genetic interaction:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16246728]"
/note="Protein component of the small (40S) ribosomal
subunit; homologous to mammalian ribosomal protein S6, no
bacterial homolog; phosphorylated on S233 by Ypk3p in a
TORC1-dependent manner, and on S232 in a TORC1/2-dependent
manner by Ypk1/2/3p; RPS6A has a paralog, RPS6B, that
arose from the whole genome duplication"
/codon_start=1
/product="40S ribosomal protein eS6 RPS6A"
/protein_id="NP_015235.1"
/db_xref="GeneID:856015"
/db_xref="SGD:S000006011"
/translation="MKLNISYPVNGSQKTFEIDDEHRIRVFFDKRIGQEVDGEAVGDE
FKGYVFKISGGNDKQGFPMKQGVLLPTRIKLLLTKNVSCYRPRRDGERKRKSVRGAIV
GPDLAVLALVIVKKGEQELEGLTDTTVPKRLGPKRANNIRKFFGLSKEDDVRDFVIRR
EVTKGEKTYTKAPKIQRLVTPQRLQRKRHQRALKVRNAQAQREAAAEYAQLLAKRLSE
RKAEKAEIRKRRASSLKA"
gene complement(<379120..>381150)
/gene="RLM1"
/locus_tag="YPL089C"
/db_xref="GeneID:856016"
mRNA complement(<379120..>381150)
/gene="RLM1"
/locus_tag="YPL089C"
/product="Rlm1p"
/transcript_id="NM_001183903.1"
/db_xref="GeneID:856016"
CDS complement(379120..381150)
/gene="RLM1"
/locus_tag="YPL089C"
/experiment="EXISTENCE:direct assay:GO:0000981 DNA-binding
transcription factor activity, RNA polymerase II-specific
[PMID:9121433]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12410835]"
/experiment="EXISTENCE:direct assay:GO:0008301 DNA
binding, bending [PMID:10064699]"
/experiment="EXISTENCE:direct assay:GO:0033554 cellular
response to stress [PMID:15116342]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:9121433]"
/experiment="EXISTENCE:direct assay:GO:0045944 positive
regulation of transcription by RNA polymerase II
[PMID:9121433|PMID:15116342|PMID:12410835]"
/experiment="EXISTENCE:mutant phenotype:GO:0007165 signal
transduction [PMID:7565726]"
/experiment="EXISTENCE:mutant phenotype:GO:0033554
cellular response to stress [PMID:12410835]"
/experiment="EXISTENCE:mutant phenotype:GO:0034605
cellular response to heat [PMID:20398213]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:12410835|PMID:20398213]"
/experiment="EXISTENCE:mutant phenotype:GO:0060256
regulation of flocculation [PMID:31441971]"
/experiment="EXISTENCE:mutant phenotype:GO:1903450
regulation of G1 to G0 transition [PMID:31141453]"
/note="MADS-box transcription factor; component of the
protein kinase C-mediated MAP kinase pathway involved in
the maintenance of cell integrity; phosphorylated and
activated by the MAP-kinase Slt2p; RLM1 has a paralog,
SMP1, that arose from the whole genome duplication"
/codon_start=1
/product="Rlm1p"
/protein_id="NP_015236.1"
/db_xref="GeneID:856016"
/db_xref="SGD:S000006010"
/translation="MGRRKIEIQRISDDRNRAVTFIKRKAGLFKKAHELSVLCQVDIA
VIILGSNNTFYEFSSVDTNDLIYHYQNDKNLLHEVKDPSDYGDFHKSASVNINQDLLR
SSMSNKPSKSNVKGMNQSENDDDENNDEDDDDHGNFERNSNMHSNKKASDKNIPSAHM
KLLSPTALISKMDGSEQNKRHPENALPPLQHLKRLKPDPLQISRTPQQQQQQNISRPY
HSSMYNLNQPSSSSSSPSTMDFPKLPSFQNSSFNGRPPPISISPNKFSKPFTNASSRT
PKQEHKINNSGSNNNDNSNYTQSPSNSLEDSIQQTVKARRKLSARPVLRVRIPNNNFS
SNSAIPSEPSSASSTSANGNSMGSSQIMKENKTSRSSKISPLSASASGPLTLQKGNNG
RMVIKLPNANAPNGSNNGNGSNNNNHPYPFGSGSSPLFSATQPYIATPLQPSNIPGGP
FQQNTSFLAQRQTQQYQQMSFKKQSQTVPLTTTLTGRPPSTFSGPETSNGPPTGSLPS
KFVHDLMSNSPNVSSISMFPDWSMGPNSAKPGNTNNPGTFPPVQTAVNNGNSSNISST
NNTNNNNNNNNNNSSNNNSNNGNDNNSNNSNNSYYSNNEDAPVNGAAISEHTTDGDSN
NQSNSSTYDAAATAYNGNTGLTPYINTAQTPLGTKFFNFSTDISGEKNSSKI"
gene <381965..>382993
/locus_tag="YPL088W"
/db_xref="GeneID:856017"
mRNA <381965..>382993
/locus_tag="YPL088W"
/product="aldo-keto reductase superfamily protein"
/transcript_id="NM_001183902.1"
/db_xref="GeneID:856017"
CDS 381965..382993
/locus_tag="YPL088W"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:31945465]"
/note="Putative aryl alcohol dehydrogenase; localizes to
cytosol; transcription is activated by paralogous
transcription factors Yrm1p and Yrr1p along with genes
involved in multidrug resistance"
/codon_start=1
/product="aldo-keto reductase superfamily protein"
/protein_id="NP_015237.1"
/db_xref="GeneID:856017"
/db_xref="SGD:S000006009"
/translation="MVLVKQVRLGNSGLKISPIVIGCMSYGSKKWADWVIEDKTQIFK
IMKHCYDKGLRTFDTADFYSNGLSERIIKEFLEYYSIKRETVVIMTKIYFPVDETLDL
HHNFTLNEFEELDLSNQRGLSRKHIIAGVENSVKRLGTYIDLLQIHRLDHETPMKEIM
KALNDVVEAGHVRYIGASSMLATEFAELQFTADKYGWFQFISSQSYYNLLYREDEREL
IPFAKRHNIGLLPWSPNARGMLTRPLNQSTDRIKSDPTFKSLHLDNLEEEQKEIINRV
EKVSKDKKVSMAMLSIAWVLHKGCHPIVGLNTTARVDEAIAALQVTLTEEEIKYLEEP
YKPQRQRC"
gene <383455..>384408
/gene="YDC1"
/locus_tag="YPL087W"
/db_xref="GeneID:856018"
mRNA <383455..>384408
/gene="YDC1"
/locus_tag="YPL087W"
/product="alkaline dihydroceramidase"
/transcript_id="NM_001183901.1"
/db_xref="GeneID:856018"
CDS 383455..384408
/gene="YDC1"
/locus_tag="YPL087W"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:10900202]"
/experiment="EXISTENCE:direct assay:GO:0017040
N-acylsphingosine amidohydrolase activity [PMID:10900202]"
/experiment="EXISTENCE:genetic interaction:GO:0046513
ceramide biosynthetic process [PMID:11694577]"
/experiment="EXISTENCE:mutant phenotype:GO:0046514
ceramide catabolic process [PMID:10900202]"
/note="Alkaline dihydroceramidase, involved in
sphingolipid metabolism; preferentially hydrolyzes
dihydroceramide to a free fatty acid and
dihydrosphingosine; has a minor reverse activity; YDC1 has
a paralog, YPC1, that arose from the whole genome
duplication"
/codon_start=1
/product="alkaline dihydroceramidase"
/protein_id="NP_015238.1"
/db_xref="GeneID:856018"
/db_xref="SGD:S000006008"
/translation="MLFSWPYPEAPIEGYWGKPTSLIDWCEENYVVSPYIAEWSNTIT
NSIFLMTAFYSTYSAWRNKLETRYILIGMGFSLVGIGSWLFHMTLQYRYQLLDELPML
YATIIPSWSIFAETQEILIKDEKKRKESSFRIQMVISFIMCGIVTILTWIYVVVQKPA
IFQVLYGILTLLVVVLSGWLTYYHVHDSFAKKNLFITMVMGMIPFVIGFICWQLDIHL
CSFWIYIRRTYLALPLGVLLELHAWWHLLTGTGVYIFVVYLQYLRILTHGNPNDFLFI
WRWGFFPELVRKGLPIGTSYSLEYLGPIVNTQVDDETKKNN"
rep_origin 384542..384789
/note="ARS1618; Autonomously Replicating Sequence"
/db_xref="SGD:S000118415"
gene complement(<384773..>386446)
/gene="ELP3"
/locus_tag="YPL086C"
/gene_synonym="HPA1; KAT9; KTI8; TOT3"
/db_xref="GeneID:856019"
mRNA complement(<384773..>386446)
/gene="ELP3"
/locus_tag="YPL086C"
/gene_synonym="HPA1; KAT9; KTI8; TOT3"
/product="Elongator subunit ELP3"
/transcript_id="NM_001183900.1"
/db_xref="GeneID:856019"
CDS complement(384773..386446)
/gene="ELP3"
/locus_tag="YPL086C"
/gene_synonym="HPA1; KAT9; KTI8; TOT3"
/EC_number="2.3.1.311"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12139626]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:12139626]"
/experiment="EXISTENCE:direct assay:GO:0033588 elongator
holoenzyme complex [PMID:11689709]"
/experiment="EXISTENCE:mutant phenotype:GO:0002098 tRNA
wobble uridine modification [PMID:18755837]"
/note="Subunit of Elongator complex; Elongator is required
for modification of wobble nucleosides in tRNA; exhibits
histone acetyltransferase activity that is directed to
histones H3 and H4; disruption confers resistance to K.
lactis zymotoxin; human homolog ELP3 can partially
complement yeast elp3 null mutant"
/codon_start=1
/product="Elongator subunit ELP3"
/protein_id="NP_015239.1"
/db_xref="GeneID:856019"
/db_xref="SGD:S000006007"
/translation="MARHGKGPKTNKKKLAPEKERFIQCCADITLELTDSLTSGTTRE
INLNGLITKYSKKYKLKQQPRLTDIINSIPDQYKKYLLPKLKAKPVRTASGIAVVAVM
CKPHRCPHIAYTGNICVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYDPYEQARGR
VEQLKQLGHSIDKVEYVLMGGTFMSLPKEYREDFIVKLHNALSGFNGNDIDEAILYSQ
QSLTKCVGITIETRPDYCTQTHLDDMLKYGCTRLEIGVQSLYEDVARDTNRGHTVRSV
CETFAVSKDAGYKVVSHMMPDLPNVGMERDIEQFKEYFENPDFRTDGLKIYPTLVIRG
TGLYELWKTGRYKSYSANALVDLVARILALVPPWTRIYRVQRDIPMPLVTSGVDNGNL
RELALARMKDLGTTCRDVRTREVGIQEVHHKVQPDQVELIRRDYYANGGWETFLSYED
PKKDILIGLLRLRKASKKYTYRKEFTSQRTSIVRELHVYGSVVPLHSRDPRKFQHQGF
GTLLMEEAERIAKEEHGSEKISVISGVGVRNYYGKLGYELDGPYMSKRI"
gene <387067..>393654
/gene="SEC16"
/locus_tag="YPL085W"
/gene_synonym="LPF1"
/db_xref="GeneID:856020"
mRNA <387067..>393654
/gene="SEC16"
/locus_tag="YPL085W"
/gene_synonym="LPF1"
/product="COPII coat assembly protein SEC16"
/transcript_id="NM_001183899.1"
/db_xref="GeneID:856020"
CDS 387067..393654
/gene="SEC16"
/locus_tag="YPL085W"
/gene_synonym="LPF1"
/experiment="EXISTENCE:direct assay:GO:0012507 ER to Golgi
transport vesicle membrane [PMID:7593161]"
/experiment="EXISTENCE:direct assay:GO:0043495
protein-membrane adaptor activity
[PMID:7593161|PMID:12235121]"
/experiment="EXISTENCE:direct assay:GO:0048208 COPII
vesicle coating [PMID:12235121]"
/experiment="EXISTENCE:direct assay:GO:0070971 endoplasmic
reticulum exit site [PMID:22675024]"
/experiment="EXISTENCE:mutant phenotype:GO:0016236
macroautophagy [PMID:11694599]"
/experiment="EXISTENCE:mutant phenotype:GO:0070973 protein
localization to endoplasmic reticulum exit site
[PMID:22675024]"
/note="COPII vesicle coat protein required for ER
transport vesicle budding; essential factor in endoplasmic
reticulum exit site (ERES) formation, as well as in
COPII-mediated ER-to-Golgi traffic; bound to periphery of
ER membranes and may act to stabilize initial COPII
complexes; interacts with Sec23p, Sec24p and Sec31p"
/codon_start=1
/product="COPII coat assembly protein SEC16"
/protein_id="NP_015240.1"
/db_xref="GeneID:856020"
/db_xref="SGD:S000006006"
/translation="MTPEAKKRKNQKKKLKQKQKKAAEKAASHSEEPLELPESTINSS
FNDDSVNRTESDIASKSDVPPVSSSTNISPANETQLEIPDTQELHHKLLNDSDQHDIT
ADSNDLPDNSIVEHDSVITQTKPAMSQEYEETAAHLSSRNPSLDVVAGELHNNNEHTQ
KIAVSAVEEDSFNEEEGENHDSIIISSLNDATPSQYNHFLPSDGNLLSPELSSGDTPT
HNVPLGTKDNEINDDEYCNDKEISLNANNVLPDELSKEEDERLKLETHVSTEEKKQDI
ADQETAENLFTSSTEPSENKIRNSGDDTSMLFQDDESDQKVPWEEDVKKDFHNENTNN
TQESAPNTDDRDKGYEGNEALKKSESCTAADERSYSEETSEDIFHGHDKQVVEGQNDF
TGKNIENESQKLMGEGNHKLPLSAEADIIEPGKDIQDQAEDLFTQSSGDLGEVLPWES
TDKNADVTSKSQEKHEDLFAASGNDEKLPWEVSDGEVSSGKTENSMQTSTEKIAEQKF
SFLENDDDLLDDDDSFLASSEEEDTVPNTDNTTNLTSKPVEEKKASRYKPIIEEEAGM
RQEQVHFTNTTGIVTPQQFHGLTKTGLGTPNQQVSVPNIVSPKPPVVKDNRSNFKINE
EKKKSDAYDFPLEIISESSKKGHAKPVAVPTQRFGSGNSFSSLDKPIPQSRKGSNNSN
RPPVIPLGTQEPRSSRTNSAISQSPVNYAFPNPYKIQQLQQAPIQSGMPLPNTNIPPP
ALKVETTVSAPPIRARGVSNASVGSSASFGARHATQYGLNNGVPPVSPYGQATINLPT
ANKYAPVSPTVQQKQYPSVVQNLGASAVNTPNFVKTHRGHTSSISSYTPNQNEHASRY
APNYQQSYQVPYTSQPVGPVAGNSSYQSQTRSSYAVPMMPQAQTSASIQPHANIQPPT
GILPLAPLRPLDPLQAATNLQPRASNITAANSLPLANLPLAENILPEIITHRATSSVA
PPRQENNPIKIDNEALLRRQFPIFHWSAANKVVYAVPPIPDQSQYMISSSIVQEIKVT
PIDQIIKPNDMLKSFPGPLGSAKLKKKDLTKWMETTIKSISENESSTDMTIWQLLEMK
LNDKVNWKNISKLLYNSDELLMYLSQPFPNGDMIPNAYRLDINCQMRVLAFLQTGNHD
EALRLALSKRDYAIALLVGSLMGKDRWSEVIQKYLYEGFTAGPNDQKELAHFLLLIFQ
VFVGNSKMAIKSFYTNNETSQWASENWKSIVAAVLINIPENNEDPLLIPPVVLEFLIE
FGIFLTKKGLTAAASTLFIIGNVPLSNEPVMADSDVIFESIGNMNTFESILWDEIYEY
IFSYDPKFKGFSSILPQKIYHASLLQEQGLNSLGTKYTDYLSSSVRKLPKKDILTINL
TRELSEVASRLSESNTGWLAKPKLSSVWGQLDKSFNKYIGGDDIDALNKKNDKKKVFD
GFTPGSSANSSTVDLTQTFTPFQAQVTSQSYVDTTALLHNAHNVPSHSVLHSKPSNVS
KGLVEANLPYTHRIGDSLQGSPQRIHNTQFAAAEPQMASLRRVRTDQHTNEKALKSQQ
ILEKKSTAYTPQFGQNHSVPMEKSNSNVPSLFADFPAPPKLGTVPSNYVSSPDLVRRE
SIISTGSEFLPPPKIGVPTKANSSQGSLMYSPSVEALPIDPVVPQVHETGYNDFGNKH
SQKSMPEDESHTSHDNSNADQNTLKDSADVTDETMDIEGPGFNDVKNLLPMEPNHQPT
STVNPIQTISDDIQPILQTNVEVRGTDASKMENSLPSIENERSSEEQPENISKSASSA
YLPSTGGLSLENRPLTQDENSISETVQSTYLPAGSISMEAKPISQVQDVPRNVNNKAS
KLVEQHMAPPKPKSTDATKMNYSPYVPQSTAASADGDESTILKTSPAIYARTHQAHAS
NPSQYFPLVNQANETASFELSESTSQAQSNGNVASENRFSPIKKAEVVEKDTFQPTIR
KASTNQYRAFKPLESDADKYNDVIEDESDDDNMSTDEAKNRKEEKKNVNMKKETKPSN
KDIDDKSNGWFGWLKKDTGDKKVYKAKLGHKNTLYYDEKLKRWVNKDATEEEKQKIIE
SSAPPPPPIVKRKDGGPKTKPRSGPINNSLPPVHATSVIPNNPITGEPLPIKTSPSPT
GPNPNNSPSPSSPISRISGVNLTSKKANGLDDLLSLAGGPKPASTRRKKKTARGYVNV
MDNIQ"
gene <394038..>396572
/gene="BRO1"
/locus_tag="YPL084W"
/gene_synonym="ASI6; LPF2; NPI3; VPS31"
/db_xref="GeneID:856021"
mRNA <394038..>396572
/gene="BRO1"
/locus_tag="YPL084W"
/gene_synonym="ASI6; LPF2; NPI3; VPS31"
/product="Bro1p"
/transcript_id="NM_001183898.1"
/db_xref="GeneID:856021"
CDS 394038..396572
/gene="BRO1"
/locus_tag="YPL084W"
/gene_synonym="ASI6; LPF2; NPI3; VPS31"
/experiment="EXISTENCE:direct assay:GO:0005515 protein
binding [PMID:34908216]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:12668726]"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:34908216|PMID:12668726|PMID:15935782]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0035800
deubiquitinase activator activity [PMID:17446860]"
/experiment="EXISTENCE:direct assay:GO:1903003 positive
regulation of protein deubiquitination [PMID:17446860]"
/experiment="EXISTENCE:direct assay:GO:1903561
extracellular vesicle [PMID:38711329]"
/experiment="EXISTENCE:genetic interaction:GO:0016579
protein deubiquitination [PMID:15326198]"
/experiment="EXISTENCE:genetic interaction:GO:0070676
intralumenal vesicle formation [PMID:23444383]"
/experiment="EXISTENCE:mutant phenotype:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:12062418]"
/experiment="EXISTENCE:mutant phenotype:GO:0007034
vacuolar transport [PMID:12668726]"
/experiment="EXISTENCE:mutant phenotype:GO:0032511 late
endosome to vacuole transport via multivesicular body
sorting pathway [PMID:34908216]"
/experiment="EXISTENCE:mutant phenotype:GO:0036010 protein
localization to endosome [PMID:15326198]"
/experiment="EXISTENCE:mutant phenotype:GO:0043328 protein
transport to vacuole involved in ubiquitin-dependent
protein catabolic process via the multivesicular body
sorting pathway [PMID:14523026]"
/experiment="EXISTENCE:mutant phenotype:GO:0070676
intralumenal vesicle formation [PMID:23444383]"
/experiment="EXISTENCE:mutant phenotype:GO:0072671
mitochondria-associated ubiquitin-dependent protein
catabolic process [PMID:21070972]"
/experiment="EXISTENCE:mutant phenotype:GO:1904669 ATP
export [PMID:26585826]"
/experiment="EXISTENCE:physical interaction:GO:0016579
protein deubiquitination [PMID:15326198]"
/note="Cytoplasmic class E vacuolar protein sorting (VPS)
factor; coordinates protein sorting and deubiquitination
in the multivesicular body (MVB) pathway by recruiting
Doa4p to endosomes; required for ESCRT-dependent transport
of ubiquitinated cargo to the vacuole; localizes to
extracellular vesicles during heat conditioning with
proposed function in thermotolerance; orthologous to
mammalian ESCRT-associated protein ALIX (PDCD6IP)"
/codon_start=1
/product="Bro1p"
/protein_id="NP_015241.1"
/db_xref="GeneID:856021"
/db_xref="SGD:S000006005"
/translation="MKPYLFDLKLKDTEKLDWKKGLSSYLKKSYGSSQWRTFYDEKAT
SELDHLRNNANGELAPSSLSEQNLKYYSFLEHLYFRLGSKGSRLKMDFTWYDAEYSSA
QKGLKYTQHTLAFEKSCTLFNIAVIFTQIARENINEDYKNSIANLTKAFSCFEYLSEN
FLNSPSVDLQSENTRFLANICHAEAQELFVLKLLNDQISSKQYTLISKLSRATCNLFQ
KCHDFMKEIDDDVAIYGEPKWKTTVTCKLHFYKSLSAYYHGLHLEEENRVGEAIAFLD
FSMQQLISSLPFKTWLVEFIDFDGFKETLEKKQKELIKDNDFIYHESVPAVVQVDSIK
ALDAIKSPTWEKILEPYMQDVANKCDSLYRGIIPLDVYEKESIYSEEKATLLRKQVEE
TETANLEYSSFIEFTNLPRLLSDLEKQFSDGNIFSNTDTQGQLMRDQIQTWCKFIQTN
EFRDIEEQMNKIVFKRKQILEILSALPNDQKENVTKLKSSLVAASNSDEKLFACVKPH
IVEINLLNDNGKIWKKFDEFNRNTPPQPSLLDIDDTKNDKILELLKQVKGHAEDLRTL
KEERSRNLSELRDEINNDDITKLLIINKGKSDVELKDLFEVELEKFEPLSTRIEATIY
KQSSMIDDIKAKLDEIFHLSNFKDKSSGEEKFLEDRKNFFDKLQEAVKSFSIFASDLP
KGIEFYDSLFNMSRDLAERVRVAKQTEDSTANSPAPPLPPLDSKASVVGGPPLLPQKS
AAFQSLSRQGLNLGDQFQNLKISAGSDLPQGPGIPPRTYEASPYAATPTMAAPPVPPK
QSQEDMYDLRRRKAVENEERELQENPTSFYNRPSVFDENMYSKYSS"
gene complement(<396702..>398105)
/gene="SEN54"
/locus_tag="YPL083C"
/db_xref="GeneID:856022"
mRNA complement(<396702..>398105)
/gene="SEN54"
/locus_tag="YPL083C"
/product="tRNA splicing endonuclease subunit SEN54"
/transcript_id="NM_001183897.1"
/db_xref="GeneID:856022"
CDS complement(396702..398105)
/gene="SEN54"
/locus_tag="YPL083C"
/experiment="EXISTENCE:direct assay:GO:0000213 tRNA-intron
lyase activity [PMID:9200603]"
/experiment="EXISTENCE:direct assay:GO:0000214 tRNA-intron
endonuclease complex [PMID:9200603]"
/experiment="EXISTENCE:direct assay:GO:0000379 tRNA-type
intron splice site recognition and cleavage
[PMID:9200603]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane
[PMID:12925762|PMID:16407407]"
/experiment="EXISTENCE:mutant phenotype:GO:0005741
mitochondrial outer membrane [PMID:12925762]"
/note="Subunit of the tRNA splicing endonuclease; tRNA
splicing endonuclease (Sen complex) is composed of Sen2p,
Sen15p, Sen34p, and Sen54p; Sen complex also cleaves the
CBP1 mRNA at the mitochondrial surface"
/codon_start=1
/product="tRNA splicing endonuclease subunit SEN54"
/protein_id="NP_015242.1"
/db_xref="GeneID:856022"
/db_xref="SGD:S000006004"
/translation="MQFAGKKTDQVTTSNPGFEEEEEEEEELQQDWSQLASLVSKNAA
LSLPKRGEKDYEPDGTNLQDLLLYNASKAMFDTISDSIRGTTVKSEVRGYYVPHKHQA
VLLKPKGSFMQTMGRADSTGELWLDFHEFVYLAERGTILPYYRLEAGSNKSSKHETEI
LLSMEDLYSLFSSQQEMDQYFVFAHLKRLGFILKPSNQEAAVKTSFFPLKKQRSNLQA
ITWRLLSLFKIQELSLFSGFFYSKWNFFFRKYTTSPQLYQGLNRLVRSVAVPKNKKEL
LDAQSDREFQKVKDIPLTFKVWKPHSNFKKRDPGLPDFQVFVYNKNDDLQHFPTYKEL
RSMFSSLDYKFEFLSEIEDDDDWETNSYVEDIPRKEYIHKRSAKSQTEKSESSMKASF
QKKTAQSSTKKKRKAYPPHIQQNRRLKTGYRSFIIAIMDNGLISFVKMSEADFGSESV
WYTPNTQKKVDQRWKKH"
gene complement(<398480..>404083)
/gene="MOT1"
/locus_tag="YPL082C"
/gene_synonym="BUR3; END10; LPF4"
/db_xref="GeneID:856023"
mRNA complement(<398480..>404083)
/gene="MOT1"
/locus_tag="YPL082C"
/gene_synonym="BUR3; END10; LPF4"
/product="DNA-binding ATPase"
/transcript_id="NM_001183896.1"
/db_xref="GeneID:856023"
CDS complement(398480..404083)
/gene="MOT1"
/locus_tag="YPL082C"
/gene_synonym="BUR3; END10; LPF4"
/experiment="EXISTENCE:direct assay:GO:0000228 nuclear
chromosome [PMID:11880621]"
/experiment="EXISTENCE:direct assay:GO:0003677 DNA binding
[PMID:22298788]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922|PMID:21976730]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0006357 regulation
of transcription by RNA polymerase II [PMID:9234740]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:9234740]"
/experiment="EXISTENCE:direct assay:GO:0042790 nucleolar
large rRNA transcription by RNA polymerase I
[PMID:17296733]"
/experiment="EXISTENCE:mutant phenotype:GO:0006364 rRNA
processing [PMID:17296733]"
/experiment="EXISTENCE:mutant phenotype:GO:0042790
nucleolar large rRNA transcription by RNA polymerase I
[PMID:17296733]"
/experiment="EXISTENCE:mutant phenotype:GO:0045892
negative regulation of DNA-templated transcription
[PMID:24459134]"
/experiment="EXISTENCE:mutant phenotype:GO:0045898
regulation of RNA polymerase II transcription
preinitiation complex assembly
[PMID:21976730|PMID:15861138]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:27226635]"
/experiment="EXISTENCE:physical interaction:GO:0017025
TBP-class protein binding [PMID:8083216]"
/note="Essential protein involved in regulation of
transcription; removes Spt15p (TBP) from DNA via its
C-terminal ATPase activity; may have a role in ensuring
that soluble TBP is available to bind TATA-less promoters;
forms a complex with TBP that binds TATA DNA with high
affinity but with altered specificity; the
Mot1p-Spt15p-DNA ternary complex contains unbent DNA;
coregulates transcription with Spt16p through assembly of
preinitiation complex and organization of nucleosomes"
/codon_start=1
/product="DNA-binding ATPase"
/protein_id="NP_015243.1"
/db_xref="GeneID:856023"
/db_xref="SGD:S000006003"
/translation="MTSRVSRLDRQVILIETGSTQVVRNMAADQMGDLAKQHPEDILS
LLSRVYPFLLVKKWETRVTAARAVGGIVAHAPSWDPNESDLVGGTNEGSPLDNAQVKL
EHEMKIKLEEATQNNQLNLLQEDHHLSSLSDWKLNEILKSGKVLLASSMNDYNVLGKA
DDNIRKQAKTDDIKQETSMLNASDKANENKSNANKKSARMLAMARRKKKMSAKNTPKH
PVDITESSVSKTLLNGKNMTNSAASLATSPTSNQLNPKLEITEQADESKLMIESTVRP
LLEQHEIVAGLVWQFQGIYELLLDNLMSENWEIRHGAALGLRELVKKHAYGVSRVKGN
TREENNLRNSRSLEDLASRLLTVFALDRFGDYVYDTVVAPVRESVAQTLAALLIHLDS
TLSIKIFNCLEQLVLQDPLQTGLPNKIWEATHGGLLGIRYFVSIKTNFLFAHGLLENV
VRIVLYGLNQSDDDVQSVAASILTPITSEFVKLNNSTIEILVTTIWSLLARLDDDISS
SVGSIMDLLAKLCDHQEVLDILKNKALEHPSEWSFKSLVPKLYPFLRHSISSVRRAVL
NLLIAFLSIKDDSTKNWLNGKVFRLVFQNILLEQNPELLQLSFDVYVALLEHYKVKHT
EKTLDHVFSKHLQPILHLLNTPVGEKGKNYAMESQYILKPSQHYQLHPEKKRSISETT
TDSDIPIPKNNEHINIDAPMIAGDITLLGLDVILNTRIMGAKAFALTLSMFQDSTLQS
FFTNVLVRCLELPFSTPRMLAGIIVSQFCSSWLQKHPEGEKLPSFVSEIFSPVMNKQL
LNRDEFPVFRELVPSLKALRTQCQSLLATFVDVGMLPQYKLPNVAIVVQGETEAGPHA
FGVETAEKVYGEYYDKMFKSMNNSYKLLAKKPLEDSKHRVLMAINSAKESAKLRTGSI
LANYASSILLFDGLPLKLNPIIRSLMDSVKEERNEKLQTMAGESVVHLIQQLLENNKV
NVSGKIVKNLCGFLCVDTSEVPDFSVNAEYKEKILTLIKESNSIAAQDDINLAKMSEE
AQLKRKGGLITLKILFEVLGPSILQKLPQLRSILFDSLSDHENEEASKVDNEQGQKIV
DSFGVLRALFPFMSDSLRSSEVFTRFPVLLTFLRSNLSVFRYSAARTFADLAKISSVE
VMAYTIREILPLMNSAGSLSDRQGSTELIYHLSLSMETDVLPYVIFLIVPLLGRMSDS
NEDVRNLATTTFASIIKLVPLEAGIADPKGLPEELVASRERERDFIQQMMDPSKAKPF
KLPIAIKATLRKYQQDGVNWLAFLNKYHLHGILCDDMGLGKTLQTICIIASDQYLRKE
DYEKTRSVESRALPSLIICPPSLTGHWENEFDQYAPFLKVVVYAGGPTVRLTLRPQLS
DADIIVTSYDVARNDLAVLNKTEYNYCVLDEGHIIKNSQSKLAKAVKEITANHRLILT
GTPIQNNVLELWSLFDFLMPGFLGTEKMFQERFAKPIAASRNSKTSSKEQEAGVLALE
ALHKQVLPFMLRRLKEDVLSDLPPKIIQDYYCELGDLQKQLYMDFTKKQKNVVEKDIE
NSEIADGKQHIFQALQYMRKLCNHPALVLSPNHPQLAQVQDYLKQTGLDLHDIINAPK
LSALRTLLFECGIGEEDIDKKASQDQNFPIQNVISQHRALIFCQLKDMLDMVENDLFK
KYMPSVTYMRLDGSIDPRDRQKVVRKFNEDPSIDCLLLTTKVGGLGLNLTGADTVIFV
EHDWNPMNDLQAMDRAHRIGQKKVVNVYRIITKGTLEEKIMGLQKFKMNIASTVVNQQ
NSGLASMDTHQLLDLFDPDNVTSQDNEEKNNGDSQAAKGMEDIANETGLTGKAKEALG
ELKELWDPSQYEEEYNLDTFIKTLR"
gene <404950..>406044
/gene="RPS9A"
/locus_tag="YPL081W"
/db_xref="GeneID:856024"
mRNA join(<404950..404956,405458..>406044)
/gene="RPS9A"
/locus_tag="YPL081W"
/product="40S ribosomal protein uS4 RPS9A"
/transcript_id="NM_001183895.1"
/db_xref="GeneID:856024"
CDS join(404950..404956,405458..406044)
/gene="RPS9A"
/locus_tag="YPL081W"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:6814480]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0019843 rRNA
binding [PMID:16540469]"
/experiment="EXISTENCE:direct assay:GO:0022627 cytosolic
small ribosomal subunit [PMID:6814480]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/experiment="EXISTENCE:direct assay:GO:0032040
small-subunit processome [PMID:15590835]"
/experiment="EXISTENCE:genetic interaction:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16246728]"
/experiment="EXISTENCE:mutant phenotype:GO:0019843 rRNA
binding [PMID:16540469]"
/experiment="EXISTENCE:mutant phenotype:GO:0030476
ascospore wall assembly [PMID:39817510]"
/note="Protein component of the small (40S) ribosomal
subunit; splicing is repressed during vegetative growth
but upregulated during meiosis; deletion impairs formation
of outer spore wall layer; homologous to mammalian
ribosomal protein S9 and bacterial S4; RPS9A has a
paralog, RPS9B, that arose from the whole genome
duplication"
/codon_start=1
/product="40S ribosomal protein uS4 RPS9A"
/protein_id="NP_015244.1"
/db_xref="GeneID:856024"
/db_xref="SGD:S000006002"
/translation="MPRAPRTYSKTYSTPKRPYESSRLDAELKLAGEFGLKNKKEIYR
ISFQLSKIRRAARDLLTRDEKDPKRLFEGNALIRRLVRVGVLSEDKKKLDYVLALKVE
DFLERRLQTQVYKLGLAKSVHHARVLITQRHIAVGKQIVNIPSFMVRLDSEKHIDFAP
TSPFGGARPGRVARRNAARKAEASGEAADEADEADEE"
gene complement(<406172..>406498)
/locus_tag="YPL080C"
/db_xref="GeneID:856025"
mRNA complement(<406172..>406498)
/locus_tag="YPL080C"
/product="uncharacterized protein"
/transcript_id="NM_001348884.1"
/db_xref="GeneID:856025"
CDS complement(406172..406498)
/locus_tag="YPL080C"
/note="hypothetical protein; conserved across S.
cerevisiae strains"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335822.1"
/db_xref="GeneID:856025"
/db_xref="SGD:S000006001"
/translation="MDGSYLTHRYCALKRPYTAHLLLLDFSKDEVTKNVWRWKGTDVK
LHKMGRKSIVAAVYNQAHPSHPPLYHLGNLNGDKYVASPPSRMKAVSLSFAFTFLYYY
FPLPTN"
gene <406636..>407539
/gene="RPL21B"
/locus_tag="YPL079W"
/db_xref="GeneID:856026"
mRNA join(<406636..406646,407068..>407539)
/gene="RPL21B"
/locus_tag="YPL079W"
/product="60S ribosomal protein eL21 RPL21B"
/transcript_id="NM_001183893.1"
/db_xref="GeneID:856026"
CDS join(406636..406646,407068..407539)
/gene="RPL21B"
/locus_tag="YPL079W"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:11983894]"
/experiment="EXISTENCE:curator inference:GO:0003735
structural constituent of ribosome [PMID:11983894]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:11983894]"
/note="Ribosomal 60S subunit protein L21B; homologous to
mammalian ribosomal protein L21, no bacterial homolog;
RPL21B has a paralog, RPL21A, that arose from the whole
genome duplication"
/codon_start=1
/product="60S ribosomal protein eL21 RPL21B"
/protein_id="NP_015246.1"
/db_xref="GeneID:856026"
/db_xref="SGD:S000006000"
/translation="MGKSHGYRSRTRYMFQRDFRKHGAVHMSTYLKIYKVGDIVDIKA
NGSIQKGMPHKFYQGKTGVVYNVTKSSVGVIINKMVGNRYLEKRLNLRVEHIKHSKCR
QEFLERVKANAAKRAEAKAQGVAVQLKRQPAQPRESRIVSTEGNVPQTLAPVPYETFI
"
gene complement(<408010..>408744)
/gene="ATP4"
/locus_tag="YPL078C"
/gene_synonym="LPF7"
/db_xref="GeneID:856027"
mRNA complement(<408010..>408744)
/gene="ATP4"
/locus_tag="YPL078C"
/gene_synonym="LPF7"
/product="F1F0 ATP synthase subunit 4"
/transcript_id="NM_001183892.1"
/db_xref="GeneID:856027"
CDS complement(408010..408744)
/gene="ATP4"
/locus_tag="YPL078C"
/gene_synonym="LPF7"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0015986 proton
motive force-driven ATP synthesis [PMID:20691145]"
/experiment="EXISTENCE:direct assay:GO:0045259
proton-transporting ATP synthase complex [PMID:18722382]"
/experiment="EXISTENCE:direct assay:GO:0046933
proton-transporting ATP synthase activity, rotational
mechanism [PMID:20691145]"
/experiment="EXISTENCE:mutant phenotype:GO:0005739
mitochondrion [PMID:18293929]"
/experiment="EXISTENCE:mutant phenotype:GO:0015986 proton
motive force-driven ATP synthesis [PMID:8068646]"
/experiment="EXISTENCE:mutant phenotype:GO:0046961
proton-transporting ATPase activity, rotational mechanism
[PMID:8068646]"
/experiment="EXISTENCE:mutant phenotype:GO:0065003
protein-containing complex assembly [PMID:18293929]"
/note="Subunit b of the stator stalk of mitochondrial F1F0
ATP synthase; ATP synthase is a large, evolutionarily
conserved enzyme complex required for ATP synthesis;
contributes to the oligomerization of the complex, which
in turn determines the shape of inner membrane cristae;
phosphorylated"
/codon_start=1
/product="F1F0 ATP synthase subunit 4"
/protein_id="NP_015247.1"
/db_xref="GeneID:856027"
/db_xref="SGD:S000005999"
/translation="MSMSMGVRGLALRSVSKTLFSQGVRCPSMVIGARYMSSTPEKQT
DPKAKANSIINAIPGNNILTKTGVLGTSAAAVIYAISNELYVINDESILLLTFLGFTG
LVAKYLAPAYKDFADARMKKVSDVLNASRNKHVEAVKDRIDSVSQLQNVAETTKVLFD
VSKETVELESEAFELKQKVELAHEAKAVLDSWVRYEASLRQLEQRQLAKSVISRVQSE
LGNPKFQEKVLQQSISEIEQLLSKLK"
gene complement(<409368..>410090)
/locus_tag="YPL077C"
/db_xref="GeneID:856028"
mRNA complement(<409368..>410090)
/locus_tag="YPL077C"
/product="uncharacterized protein"
/transcript_id="NM_001183891.1"
/db_xref="GeneID:856028"
CDS complement(409368..410090)
/locus_tag="YPL077C"
/note="hypothetical protein; regulates PIS1 expression;
mutant displays spore wall assembly defect in ether
sensitivity screen; YPL077C is not an essential gene;
YPL077C has a paralog, YBR197C, that arose from the whole
genome duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015248.1"
/db_xref="GeneID:856028"
/db_xref="SGD:S000005998"
/translation="MKDLQKKSSVRRQITNEDDERYGEDSIHDLPRTIPNVNPYIRNS
GFRPSYSSQIPSTRSLFNNYYNRSSANTVGNDTIDTDSVSYNGVAKFRRNSVDIPLQT
HNRLEVRPIIDRQDYLWREIDALDDVKRQAQATELYDQFPPGFENKLMQLRQAHSKLL
QVLRDRNAKIEEEQRREVAVATAAAMMTRTPSPTGKSVGDEATSNNMHSSSAIRNPNG
PTVDPEEGKYIQELVNTIRELQ"
gene <410443..>411285
/gene="GPI2"
/locus_tag="YPL076W"
/gene_synonym="GCR4"
/db_xref="GeneID:856029"
mRNA <410443..>411285
/gene="GPI2"
/locus_tag="YPL076W"
/gene_synonym="GCR4"
/product="phosphatidylinositol
N-acetylglucosaminyltransferase"
/transcript_id="NM_001183890.1"
/db_xref="GeneID:856029"
CDS 410443..411285
/gene="GPI2"
/locus_tag="YPL076W"
/gene_synonym="GCR4"
/EC_number="2.4.1.198"
/experiment="EXISTENCE:curator inference:GO:0017176
phosphatidylinositol N-acetylglucosaminyltransferase
activity []"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0006506 GPI anchor
biosynthetic process [PMID:7768896]"
/experiment="EXISTENCE:mutant phenotype:GO:0006506 GPI
anchor biosynthetic process [PMID:7768896]"
/experiment="EXISTENCE:physical interaction:GO:0000506
glycosylphosphatidylinositol-N-
acetylglucosaminyltransferase (GPI-GnT) complex
[PMID:15163411]"
/note="Protein involved in the synthesis of GlcNAc-PI;
GlcNAc-PI is the first intermediate in the synthesis of
glycosylphosphatidylinositol (GPI) anchors; homologous to
the human PIG-C protein; GlcNAc-PI stands for
N-acetylglucosaminyl phosphatidylinositol"
/codon_start=1
/product="phosphatidylinositol
N-acetylglucosaminyltransferase"
/protein_id="NP_015249.1"
/db_xref="GeneID:856029"
/db_xref="SGD:S000005997"
/translation="MTRSPWKRLLWLKQEYPDNYTDPSFIELRARQKAESNQKSDRKL
SEAARAQIRLDFISFYQTILNTSFIYITFTYIYYYGFDPIPPTIFLSFITLIISRTKV
DPLLSSFMDVKSSLIITFAMLTLSPVLKSLSKTTASDSIWTLSFWLTLWYIFVISSTK
SKDKPSNLSTNILVALVAVLSSRLSTTIDVFCFLLICIQLNIILPTYLSVTNKVVPII
SNIIVYSFLNVALGWIYMLLIFFASVFYITVLPKWFIYWKINYHKRDNDLLSTWDART
PILD"
gene <412254..>415362
/gene="GCR1"
/locus_tag="YPL075W"
/gene_synonym="LPF10; SIT3"
/db_xref="GeneID:856030"
mRNA join(<412254..412261,413013..>415362)
/gene="GCR1"
/locus_tag="YPL075W"
/gene_synonym="LPF10; SIT3"
/product="transcription regulator GCR1"
/transcript_id="NM_001183889.1"
/db_xref="GeneID:856030"
CDS join(412254..412261,413013..415362)
/gene="GCR1"
/locus_tag="YPL075W"
/gene_synonym="LPF10; SIT3"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding
[PMID:10455229|PMID:9335590|PMID:1946357|PMID:7808400|PMID
:12655409|PMID:1588965|PMID:9271107]"
/experiment="EXISTENCE:direct assay:GO:0001228 DNA-binding
transcription activator activity, RNA polymerase
II-specific [PMID:9335590]"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:15817685]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:12655409]"
/experiment="EXISTENCE:mutant phenotype:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:1588965]"
/experiment="EXISTENCE:mutant phenotype:GO:0001228
DNA-binding transcription activator activity, RNA
polymerase II-specific [PMID:9271107]"
/experiment="EXISTENCE:mutant phenotype:GO:0006110
regulation of glycolytic process
[PMID:3547083|PMID:7808400|PMID:11333224|PMID:10510295]"
/experiment="EXISTENCE:mutant phenotype:GO:0034728
nucleosome organization [PMID:2854200]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:10455229|PMID:10510295|PMID:11333224|PMID:3547083|PM
ID:7808400|PMID:9335590|PMID:15789351]"
/experiment="EXISTENCE:mutant phenotype:GO:0060963
positive regulation of ribosomal protein gene
transcription by RNA polymerase II [PMID:11333224]"
/experiment="EXISTENCE:mutant phenotype:GO:0061435
positive regulation of transcription from a mobile element
promoter [PMID:9271107]"
/note="Transcriptional activator of genes involved in
glycolysis; DNA-binding protein that interacts and
functions with the transcriptional activator Gcr2p;
contains a long intron, which allows multiple RNA and
protein isoforms with varying expression levels depending
on glucose availability"
/codon_start=1
/product="transcription regulator GCR1"
/protein_id="NP_015250.1"
/db_xref="GeneID:856030"
/db_xref="SGD:S000005996"
/translation="MVCTSTSSNFYSIAQYILQSYFKVNVDSLNSLKLVDLIVDQTYP
DSLTLRKLNEGATGQPYDYFNTVSRDADISKCPIFALTIFFVIRWSHPNPPISIENFT
TVPLLDSNFISLNSNPLLYIQNQNPNSNSSVKVSRSQTFEPSKELIDLVFPWLSYLKQ
DMLLIDRTNYKLYSLCELFEFMGRVAIQDLRYLSQHPLLLPNIVTFISKFIPELFQNE
EFKGIGSIKNSNNNALNNVTGIETQFLNPSTEEVSQKVDSYFMELSKKLTTENIRLSQ
EITQLKADMNSVGNVCNQILLLQRQLLSGNQAIGSKSENIVSSTGGGILILDKNSINS
NVLSNLVQSIDPNHSKPNGQAQTHQRGPKGQSHAQVQSTNSPALAPINMFPSLSNSIQ
PMLGTLAPQPQDIVQKRKLPLPGSIASAATGSPFSPSPVGESPYSKRFKLDDKPTPSQ
TALDSLLTKSISSPRLPLSTLANTAVTESFRSPQQFQHSPDFVVGGSSSSTTENNSKK
VNEDSPSSSSKLAERPRLPNNDSTTSMPESPTEVAGDDVDREKPPESSKSEPNDNSPE
SKDPEKNGKNSNPLGTDADKPVPISNIHNSTEAANSSGTVTKTAPSFPQSSSKFEIIN
KKDTKAGPNEAIKYKLSRENKTIWDLYAEWYIGLNGKSSIKKLIENYGWRRWKVSEDS
HFFPTRRIIMDYIETECDRGIKLGRFTNPQQPREDIRKILVGDLEKFRINNGLTLNSL
SLYFRNLTKNNKEICIFENFKNWNVRSMTEEEKLKYCKRRHNTPS"
gene <415763..>418027
/gene="YTA6"
/locus_tag="YPL074W"
/db_xref="GeneID:856031"
mRNA <415763..>418027
/gene="YTA6"
/locus_tag="YPL074W"
/product="putative AAA family ATPase YTA6"
/transcript_id="NM_001183888.1"
/db_xref="GeneID:856031"
CDS 415763..418027
/gene="YTA6"
/locus_tag="YPL074W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005938 cell cortex
[PMID:11553699]"
/experiment="EXISTENCE:mutant phenotype:GO:0045727
positive regulation of translation [PMID:32639961]"
/note="Putative ATPase of the CDC48/PAS1/SEC18 (AAA)
family; localized to the cortex of mother cells but not to
daughter cells; relocalizes from cytoplasm to plasma
membrane foci upon DNA replication stress"
/codon_start=1
/product="putative AAA family ATPase YTA6"
/protein_id="NP_015251.1"
/db_xref="GeneID:856031"
/db_xref="SGD:S000005995"
/translation="MAHEKFSIPENFTLAQSLQLLYSVVKNQYKNLADLIINSKGNKD
TVTYGKIHKNLDTLLVYVNEGLRKIEKTYTLKKGLGNLVVDHPELRSIIEDFQILGQD
IRIARRKAETLMAEGNGSPSLSSSSSVLGLGTGNGLRFPKLWRMGSKRDKLKEADEKE
AKINKQADNIRRARKLEEEKKLGAKLQYERDLELQREKLIELKVKEKVEFEVAQKLEE
ERVKREEEERKHREQAEKKRISTLKHERKTNYKSRASLDNFSSSSKSSGKIDNSLIKR
RSLDVVRTSGERVRTPVRKSMEAAEIGMAAQLAWSQYQNGANHSKVSNNGSHSNELQI
RYKPTPPLKKRYDYKKPTVNRPIIKSPTLNRQNSKSSRNIPTNSKLKASKSNTNKVSR
RNEQNLEPSSPVLVSATAVPAESKPMRSKSGTPDKESSASSSLDSRKEDILKSVQGVD
RNACEQILNEILVTDEKVYWEDIAGLRNAKNSLKEAVVYPFLRPDLFKGLREPVRGML
LFGPPGTGKTMIAKAVATESNSTFFSVSASSLLSKYLGESEKLVRALFYMAKKLSPSI
IFIDEIDSMLTARSDNENESSRRIKTELLIQWSSLSSATAQSEDRNNTLDSRVLVLGA
TNLPWAIDDAARRRFSRKLYIPLPDYETRLYHLKRLMAKQKNSLQDLDYELITEMTEG
FSGSDLTSLAKEAAMEPIRDLGDKLMFADFDKIRGIEIKDFQNALLTIKKSVSSESLQ
KYEEWSSKFGSNGS"
rep_origin 418138..418364
/note="ARS1619; Autonomously Replicating Sequence"
/db_xref="SGD:S000118416"
gene <418512..>420011
/gene="UBP16"
/locus_tag="YPL072W"
/db_xref="GeneID:856032"
mRNA <418512..>420011
/gene="UBP16"
/locus_tag="YPL072W"
/product="putative ubiquitin-specific protease UBP16"
/transcript_id="NM_001183886.1"
/db_xref="GeneID:856032"
CDS 418512..420011
/gene="UBP16"
/locus_tag="YPL072W"
/EC_number="3.4.19.12"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane
[PMID:16407407|PMID:12914939]"
/note="Putative deubiquitinating enzyme anchored to the
outer mitochondrial membrane; probably not important for
general mitochondrial functioning, but may perform a more
specialized function at mitochondria"
/codon_start=1
/product="putative ubiquitin-specific protease UBP16"
/protein_id="NP_015253.1"
/db_xref="GeneID:856032"
/db_xref="SGD:S000005993"
/translation="MSWIKNVTESPTSLIKKVSCGLIIAASLYAIAPSLSALVFGDSK
QSIGKYTTVGLINRGNDCFITSSLQGLAGIPRFVEYLKRIRTVLLELETKLSNNAKGD
NPTVDNTTRHSRLENSSNSLAPLHESLTSLILDLISVKDRKTSISPKIVINTLESIFK
SKISSKQNDAHEFTLILLQTLQEERSKLIDYSKQICNLNIPKFPFEGETSKFLVCLKC
KGLSEPSYKQTFIRELSVPQQTSENLSNILAHDETEIIDDYSCLICQIRAILNHEEYR
NFKDCTPDEILMLDRLKNYATKAPINENLPFEVEQYVKRYSKGNLQVSNIKGKVIKKD
VVVQLPDILIVHLSRSTFNGITYSRNPCNVKFGERITLSEYTLAESGTITENRQVKYN
LKSVVKHTGSHSSGHYMCYRRKTEIRFGKEDESSFRRAPVVNNEVNKNREQNVAHNDY
KKSRYKKVKNALRYPYWQISDTAIKESTASTVLNEQKYAYMLYYERVNK"
gene complement(<420048..>420518)
/locus_tag="YPL071C"
/db_xref="GeneID:856034"
mRNA complement(<420048..>420518)
/locus_tag="YPL071C"
/product="uncharacterized protein"
/transcript_id="NM_001183885.1"
/db_xref="GeneID:856034"
CDS complement(420048..420518)
/locus_tag="YPL071C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to both the cytoplasm and
the nucleus"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015254.1"
/db_xref="GeneID:856034"
/db_xref="SGD:S000005992"
/translation="MSSRFARSNGNPNHIRKRNHSPDPIGIDNYKRKRLIIDLENLSL
NDKGPKNGHADDNNLIHNNIVFTDAIDDKVLKEIIKCSTSKRGDNDLFYDKIWERLRE
KRLQIIKWVDYKEIAYLSWWKWFHNQMTSKYTYDGEADTDVEMMAVDTDVDMDA"
gene <420948..>422786
/gene="MUK1"
/locus_tag="YPL070W"
/db_xref="GeneID:856035"
mRNA <420948..>422786
/gene="MUK1"
/locus_tag="YPL070W"
/product="guanine nucleotide exchange factor MUK1"
/transcript_id="NM_001183884.1"
/db_xref="GeneID:856035"
CDS 420948..422786
/gene="MUK1"
/locus_tag="YPL070W"
/experiment="EXISTENCE:direct assay:GO:0005085
guanyl-nucleotide exchange factor activity
[PMID:23612966|PMID:23264632]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762|PMID:23612966]"
/experiment="EXISTENCE:genetic interaction:GO:0006895
Golgi to endosome transport [PMID:23612966]"
/experiment="EXISTENCE:genetic interaction:GO:0032511 late
endosome to vacuole transport via multivesicular body
sorting pathway [PMID:23612966]"
/experiment="EXISTENCE:genetic interaction:GO:0036010
protein localization to endosome [PMID:23264632]"
/note="Guanine nucleotide exchange factor (GEF); involved
in vesicle-mediated vacuolar transport, including
Golgi-endosome trafficking and sorting through the
multivesicular body (MVB); specifically stimulates the
intrinsic guanine nucleotide exchange activity of Rab
family members (Vps21p/Ypt52p/Ypt53p); partially redundant
with GEF VPS9; required for localization of the CORVET
complex to endosomes; contains a VPS9 domain"
/codon_start=1
/product="guanine nucleotide exchange factor MUK1"
/protein_id="NP_015255.1"
/db_xref="GeneID:856035"
/db_xref="SGD:S000005991"
/translation="MARQLFTPPITNPRFDPNQSIRESYKNTTGGMQFQQNLHEDQND
NERSSCDGDENSTTGERLENNKSPILTKQEIDEALNTVTNLPPELSKLIDIFIDDLKQ
PKYVRPLSVLQLSSLFQSFYIKFDKASFQHVSSANNNGYYFSGGGSSSFLAAKETLSS
GLSGIFGRSRSSSGNSLMRPRRSSSLFSNESISNSTNATQMLSPEEIKKQLKINELNN
MKIEKYMELCERDVFKKILIVGTSVSSPNKMKTFKPHQLQTFKVGNLFRNSVEFTEYN
KLLNEKILCLSKLSTMNKINLIKFLSLNNGIDPEPKFEEIKDILYEFTYHSISPCEKI
KALLKLHEIMTYSQEMSNDDYLSLLIYYIITIVPRDIFLNAEFIRLFRYKKKLVETES
FALTNLEAALVFVEGLTKNDFSNELQDKLTVNESKILENSISSRVSLPSKTAIMHKNN
GNNGSNLGDIVTPTIQRPDVTRSNSYDGFRTVFDSSLKNIIGKIRSYTPPHPNNTSNN
NLHSSNNLNIPRSSSQLSMELSNRDTTEMSRDGSRSTSSSSRSSASLEHGNREFTGDL
TVTASINGADKKEFQKSWKKYKGYKFEDLTICELRDLFEIYQKMMQ"
rep_origin 422786..422885
/note="ARS1620; Putative replication origin; identified in
multiple array studies, not yet confirmed by plasmid-based
assay"
/db_xref="SGD:S000130200"
gene complement(<422885..>423892)
/gene="BTS1"
/locus_tag="YPL069C"
/db_xref="GeneID:856036"
mRNA complement(<422885..>423892)
/gene="BTS1"
/locus_tag="YPL069C"
/product="farnesyltranstransferase"
/transcript_id="NM_001183883.1"
/db_xref="GeneID:856036"
CDS complement(422885..423892)
/gene="BTS1"
/locus_tag="YPL069C"
/EC_number="2.5.1.1"
/EC_number="2.5.1.10"
/EC_number="2.5.1.29"
/experiment="EXISTENCE:direct assay:GO:0004311
geranylgeranyl diphosphate synthase activity
[PMID:7665600]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0016114 terpenoid
biosynthetic process [PMID:7665600]"
/experiment="EXISTENCE:mutant phenotype:GO:0016114
terpenoid biosynthetic process [PMID:7665600]"
/note="Geranylgeranyl diphosphate synthase (GGPPS);
increases the intracellular pool of geranylgeranyl
diphosphate, suppressor of bet2 mutation that causes
defective geranylgeranylation of small GTP-binding
proteins that mediate vesicular traffic"
/codon_start=1
/product="farnesyltranstransferase"
/protein_id="NP_015256.1"
/db_xref="GeneID:856036"
/db_xref="SGD:S000005990"
/translation="MEAKIDELINNDPVWSSQNESLISKPYNHILLKPGKNFRLNLIV
QINRVMNLPKDQLAIVSQIVELLHNSSLLIDDIEDNAPLRRGQTTSHLIFGVPSTINT
ANYMYFRAMQLVSQLTTKEPLYHNLITIFNEELINLHRGQGLDIYWRDFLPEIIPTQE
MYLNMVMNKTGGLFRLTLRLMEALSPSSHHGHSLVPFINLLGIIYQIRDDYLNLKDFQ
MSSEKGFAEDITEGKLSFPIVHALNFTKTKGQTEQHNEILRILLLRTSDKDIKLKLIQ
ILEFDTNSLAYTKNFINQLVNMIKNDNENKYLPDLASHSDTATNLHDELLYIIDHLSE
L"
gene complement(<424215..>425096)
/locus_tag="YPL068C"
/db_xref="GeneID:856037"
mRNA complement(<424215..>425096)
/locus_tag="YPL068C"
/product="uncharacterized protein"
/transcript_id="NM_001183882.1"
/db_xref="GeneID:856037"
CDS complement(424215..425096)
/locus_tag="YPL068C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to the nucleus and is
induced in response to the DNA-damaging agent MMS"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015257.1"
/db_xref="GeneID:856037"
/db_xref="SGD:S000005989"
/translation="MHMQLRKRKRVDYSGRNQTSDPPSTTTAAVPSIIVPKKRKVVAQ
NMVSPAIRATTTTLGTSNIIIPKPLQRPKFHNSASLSSPDDDPEKISVLEVQKNLSNL
IKRQQRLFYKDIHKPTLAGLKNFEMLRLPNDLKLLQNIVNLLYSFEQLNSDSKTRPVT
TSKLKASSQAHSDKLKKMLAERKPPFSHPSHSGTAYHNDIIHEIANLHSINLVDLINL
EVYNNNCHTNNTALQTTANSLTLNSIIKKLDKPILKERNNSLVWPHKSRFKAKRNQPS
PGQSLINNTDITLYNDV"
gene complement(<425251..>425847)
/gene="HTC1"
/locus_tag="YPL067C"
/db_xref="GeneID:856038"
mRNA complement(<425251..>425847)
/gene="HTC1"
/locus_tag="YPL067C"
/product="Htc1p"
/transcript_id="NM_001183881.1"
/db_xref="GeneID:856038"
CDS complement(425251..425847)
/gene="HTC1"
/locus_tag="YPL067C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to the cytoplasm; YPL067C
is not an essential gene"
/codon_start=1
/product="Htc1p"
/protein_id="NP_015258.1"
/db_xref="GeneID:856038"
/db_xref="SGD:S000005988"
/translation="MQQDIVNDHQEEAQGWKWEQIKEIIESGELARLKRSRQMTDKYH
EHKKRTAGLDMNQYVLQKLGWSLDEPQLENAAAKAFSSSTLYAVRANDFPYNFEPGVV
HLVLWSKVALPVHSPDKAVREAARARMNAFLQAQPLLRPLLSSGHVAWFVNYPELQSV
ARIFHAHVLLFFPRERYSAEQVKTTVDDILSHGFEPLA"
gene <426233..>427672
/gene="RGL1"
/locus_tag="YPL066W"
/db_xref="GeneID:856039"
mRNA <426233..>427672
/gene="RGL1"
/locus_tag="YPL066W"
/product="Rgl1p"
/transcript_id="NM_001183880.1"
/db_xref="GeneID:856039"
CDS 426233..427672
/gene="RGL1"
/locus_tag="YPL066W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0097271 protein
localization to bud neck [PMID:22344253]"
/experiment="EXISTENCE:physical interaction:GO:0097271
protein localization to bud neck [PMID:22344253]"
/note="Regulator of Rho1p signaling, cofactor of Tus1p;
required for the localization of Tus1p during all phases
of cytokinesis; green fluorescent protein (GFP)-fusion
protein localizes to the bud neck and cytoplasm; null
mutant is viable and exhibits growth defect on a
non-fermentable (respiratory) carbon source"
/codon_start=1
/product="Rgl1p"
/protein_id="NP_015259.1"
/db_xref="GeneID:856039"
/db_xref="SGD:S000005987"
/translation="MTHPVISLKPSYNSVIRGCPGLPDTLPRIECQLRVRSNNSLPFK
LVKIEIVLKTIEIYFNKNLYSSNNSSFTPFNRPSDPSNGHSDTSNQNISIHYKKNIVL
SHPTHDGDDLNNDLIGIDIPLTIGLPDDIKETNYNPKFGKTQTFLDCTVFYTEVGGGS
SNKKRNFLYPVNVERYTYLPSPSYFRPINRSNITSPDQKFLISYSIENPCVSMNNDTL
KLSISIRLNPFPNNATTPSSNDFDVSTPTLFSTKKKFKSKLKLKSITTQILEYLEILK
NQSEFSSTQTTNILQTSVRQVDQIISMNSMIFQFNLKIFTKDKILQSFRSSESSCPET
KVLINKIDDIPLQYHSSITTIGQHFNVSHYLSIRFKFNKSLKNFEINHPLIISFWSVS
QLPLIENLILQERQTAKFAKKFYKNFGRIKNTSNNNNSSNCLEYPSLPPIIYNFNDPE
TNNRFNILYSQKDPSRTDPSKLRRVPVIQ"
gene <427927..>428655
/gene="VPS28"
/locus_tag="YPL065W"
/gene_synonym="VPL13; VPT28"
/db_xref="GeneID:856040"
mRNA <427927..>428655
/gene="VPS28"
/locus_tag="YPL065W"
/gene_synonym="VPL13; VPT28"
/product="ESCRT-I subunit protein VPS28"
/transcript_id="NM_001183879.1"
/db_xref="GeneID:856040"
CDS 427927..428655
/gene="VPS28"
/locus_tag="YPL065W"
/gene_synonym="VPL13; VPT28"
/experiment="EXISTENCE:direct assay:GO:0000813 ESCRT I
complex [PMID:15044434]"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:11511343]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0044877
protein-containing complex binding [PMID:17215868]"
/experiment="EXISTENCE:mutant phenotype:GO:0006612 protein
targeting to membrane [PMID:11511343]"
/experiment="EXISTENCE:mutant phenotype:GO:0006623 protein
targeting to vacuole [PMID:11511343]"
/experiment="EXISTENCE:mutant phenotype:GO:1904669 ATP
export [PMID:26585826]"
/experiment="EXISTENCE:physical interaction:GO:0000813
ESCRT I complex [PMID:16615894|PMID:11511343]"
/experiment="EXISTENCE:physical interaction:GO:0044877
protein-containing complex binding [PMID:17215868]"
/note="Component of the ESCRT-I complex; complex is
involved in ubiquitin-dependent sorting of proteins into
the endosome; conserved C-terminal domain interacts with
ESCRT-III subunit Vps20p; other members include Stp22p,
Srn2p, Vps28p, and Mvb12p"
/codon_start=1
/product="ESCRT-I subunit protein VPS28"
/protein_id="NP_015260.1"
/db_xref="GeneID:856040"
/db_xref="SGD:S000005986"
/translation="MQKHNIKLNQNQDISQLFHDEVPLFDNSITSKDKEVIETLSEIY
SIVITLDHVEKAYLKDSIDDTQYTNTVDKLLKQFKVYLNSQNKEEINKHFQSIEAFCD
TYNITASNAITRLERGIPITAEHAISTTTSAPSGDNKQSSSSDKKFNAKYVAEATGNF
ITVMDALKLNYNAKDQLHPLLAELLISINRVTRDDFENRSKLIDWIVRINKLSIGDTL
TETQIRELLFDLELAYKSFYALLD"
gene complement(<428711..>429616)
/gene="CWC27"
/locus_tag="YPL064C"
/db_xref="GeneID:856041"
mRNA complement(<428711..>429616)
/gene="CWC27"
/locus_tag="YPL064C"
/product="putative peptidylprolyl isomerase CWC27"
/transcript_id="NM_001183878.1"
/db_xref="GeneID:856041"
CDS complement(428711..429616)
/gene="CWC27"
/locus_tag="YPL064C"
/EC_number="5.2.1.8"
/experiment="EXISTENCE:direct assay:GO:0005684 U2-type
spliceosomal complex [PMID:11884590]"
/note="Component of a complex containing Cef1p; putatively
involved in pre-mRNA splicing; has similarity to S. pombe
Cwf27p; protein abundance increases in response to DNA
replication stress"
/codon_start=1
/product="putative peptidylprolyl isomerase CWC27"
/protein_id="NP_015261.1"
/db_xref="GeneID:856041"
/db_xref="SGD:S000005985"
/translation="MSSNIEPQTTAKCILYTTKGNIAIELWAKECPETCKRFLSMLSD
GTFTNGEFKELKPTQWLMFNANSTGEYRTVAEEKNPRIRFNRDGLLGWDRRRNTWFIT
VLADSKHVLNDCNVFGKIVGKSIYIFREILGGEIEASSRDNDVKRFMYPAVLKDVEIT
IPFFEDIFGSKRRLEDNEKKEQEPAKKLVKSAKVKMVYEDEQEDDDGDVQKLKPRKRM
ILPAWIKDDSRSEGIKLDASLDQPQEALIREKTELHDNVDEATTKETESQENIKEEPM
DKRERETLAMLSKFQERIKNKNILK"
gene <429939..>431369
/gene="TIM50"
/locus_tag="YPL063W"
/db_xref="GeneID:856042"
mRNA <429939..>431369
/gene="TIM50"
/locus_tag="YPL063W"
/product="protein translocase subunit TIM50"
/transcript_id="NM_001183877.1"
/db_xref="GeneID:856042"
CDS 429939..431369
/gene="TIM50"
/locus_tag="YPL063W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005744 TIM23
mitochondrial import inner membrane translocase complex
[PMID:12437924|PMID:12437925]"
/experiment="EXISTENCE:direct assay:GO:0030943
mitochondrion targeting sequence binding
[PMID:18418384|PMID:19144822]"
/experiment="EXISTENCE:direct assay:GO:0046902 regulation
of mitochondrial membrane permeability [PMID:16763150]"
/experiment="EXISTENCE:mutant phenotype:GO:0008320 protein
transmembrane transporter activity [PMID:16763150]"
/experiment="EXISTENCE:mutant phenotype:GO:0030150 protein
import into mitochondrial matrix
[PMID:12437924|PMID:12437925]"
/note="Essential component of the TIM23 complex; acts as
receptor for the translocase of the inner mitochondrial
membrane (TIM23) complex guiding incoming precursors from
the TOM complex; may control the gating of the
Tim23p-Tim17p channel"
/codon_start=1
/product="protein translocase subunit TIM50"
/protein_id="NP_015262.1"
/db_xref="GeneID:856042"
/db_xref="SGD:S000005984"
/translation="MLSILRNSVRLNSRALRVVPSAANTLTSVQASRRLLTSYSSFLQ
KETKDDKPKSILTDDMLFKAGVDVDEKGQGKNEETSGEGGEDKNEPSSKSEKSRRKRQ
TSTDIKREKYANWFYIFSLSALTGTAIYMARDWEPQESEELKKDIDNGYTLSLMYKRF
KARFNSMFTYFQEPPFPDLLPPPPPPPYQRPLTLVITLEDFLVHSEWSQKHGWRTAKR
PGADYFLGYLSQYYEIVLFSSNYMMYSDKIAEKLDPIHAFVSYNLFKEHCVYKDGVHI
KDLSKLNRDLSKVIIIDTDPNSYKLQPENAIPMEPWNGEADDKLVRLIPFLEYLATQQ
TKDVRPILNSFEDKKNLAEEFDHRVKKLKDKFYGDHKSGGNWAMTALGLGNSLGGSTK
FPLDLIHEEGQKNYLMFMKMIEEEKEKIRIQQEQMGGQTFTLKDYVEGNLPSPEEQMK
IQLEKQKEVDALFEEEKKKKKIAESK"
gene <431895..>432299
/locus_tag="YPL062W"
/db_xref="GeneID:856043"
mRNA <431895..>432299
/locus_tag="YPL062W"
/product="uncharacterized protein"
/transcript_id="NM_001348885.1"
/db_xref="GeneID:856043"
CDS 431895..432299
/locus_tag="YPL062W"
/experiment="EXISTENCE:mutant phenotype:GO:0032443
regulation of ergosterol biosynthetic process
[PMID:30683746]"
/experiment="EXISTENCE:mutant phenotype:GO:0051485
terpenoid biosynthetic process, mevalonate-dependent
[PMID:30683746]"
/note="hypothetical protein; conserved across S.
cerevisiae strains; deletion decreases transcription of
downstream gene ALD6; YPL062W is not an essential gene;
null mutant shows decrease in terpenoid production;
homozygous diploid mutant shows a decrease in glycogen
accumulation"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335823.1"
/db_xref="GeneID:856043"
/db_xref="SGD:S000005983"
/translation="MIELDYVKGEDTIVEATSTSPWLMRSPLARAAEKRGSGLFFDIN
EGHGQHRDVIVAYGVSKPKRRSPHPHGNKAADKRKTTEKEPERKKRVGAPRTCKKICI
QLLYRFNFTVLGIGNVCNIDLLLGTVSATNAI"
gene <432588..>434090
/gene="ALD6"
/locus_tag="YPL061W"
/gene_synonym="ALD1"
/db_xref="GeneID:856044"
mRNA <432588..>434090
/gene="ALD6"
/locus_tag="YPL061W"
/gene_synonym="ALD1"
/product="aldehyde dehydrogenase (NADP(+)) ALD6"
/transcript_id="NM_001183875.1"
/db_xref="GeneID:856044"
CDS 432588..434090
/gene="ALD6"
/locus_tag="YPL061W"
/gene_synonym="ALD1"
/EC_number="1.2.1.4"
/experiment="EXISTENCE:direct assay:GO:0004030 aldehyde
dehydrogenase [NAD(P)+] activity [PMID:9473035]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:24769239|PMID:14576278|PMID:16806052|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:16806052]"
/experiment="EXISTENCE:genetic interaction:GO:0006740
NADPH regeneration [PMID:12584194]"
/experiment="EXISTENCE:mutant phenotype:GO:0004030
aldehyde dehydrogenase [NAD(P)+] activity [PMID:9392076]"
/experiment="EXISTENCE:mutant phenotype:GO:0005829 cytosol
[PMID:9392076]"
/experiment="EXISTENCE:mutant phenotype:GO:0009651
response to salt stress [PMID:15146068]"
/experiment="EXISTENCE:mutant phenotype:GO:0019413 acetate
biosynthetic process [PMID:11870853]"
/note="Cytosolic aldehyde dehydrogenase; activated by Mg2+
and utilizes NADP+ as the preferred coenzyme; required for
conversion of acetaldehyde to acetate; constitutively
expressed; localizes to the mitochondrial outer surface
upon oxidative stress; negative regulator of nonselective
autophagy; Stb5p directly regulates the expression of ALD6
mRNA and directly binds to the ALD6 promoter"
/codon_start=1
/product="aldehyde dehydrogenase (NADP(+)) ALD6"
/protein_id="NP_015264.1"
/db_xref="GeneID:856044"
/db_xref="SGD:S000005982"
/translation="MTKLHFDTAEPVKITLPNGLTYEQPTGLFINNKFMKAQDGKTYP
VEDPSTENTVCEVSSATTEDVEYAIECADRAFHDTEWATQDPRERGRLLSKLADELES
QIDLVSSIEALDNGKTLALARGDVTIAINCLRDAAAYADKVNGRTINTGDGYMNFTTL
EPIGVCGQIIPWNFPIMMLAWKIAPALAMGNVCILKPAAVTPLNALYFASLCKKVGIP
AGVVNIVPGPGRTVGAALTNDPRIRKLAFTGSTEVGKSVAVDSSESNLKKITLELGGK
SAHLVFDDANIKKTLPNLVNGIFKNAGQICSSGSRIYVQEGIYDELLAAFKAYLETEI
KVGNPFDKANFQGAITNRQQFDTIMNYIDIGKKEGAKILTGGEKVGDKGYFIRPTVFY
DVNEDMRIVKEEIFGPVVTVAKFKTLEEGVEMANSSEFGLGSGIETESLSTGLKVAKM
LKAGTVWINTYNDFDSRVPFGGVKQSGYGREMGEEVYHAYTEVKAVRIKL"
gene <434523..>435764
/gene="MFM1"
/locus_tag="YPL060W"
/gene_synonym="LPE10"
/db_xref="GeneID:856045"
mRNA <434523..>435764
/gene="MFM1"
/locus_tag="YPL060W"
/gene_synonym="LPE10"
/product="Mfm1p"
/transcript_id="NM_001183874.1"
/db_xref="GeneID:856045"
CDS 434523..435764
/gene="MFM1"
/locus_tag="YPL060W"
/gene_synonym="LPE10"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:11254124]"
/experiment="EXISTENCE:mutant phenotype:GO:0015095
magnesium ion transmembrane transporter activity
[PMID:11254124]"
/experiment="EXISTENCE:mutant phenotype:GO:0045016
mitochondrial magnesium ion transmembrane transport
[PMID:11254124]"
/note="Mitochondrial inner membrane magnesium transporter;
involved in maintenance of mitochondrial magnesium
concentrations and membrane potential; indirectly affects
splicing of group II introns; functionally and
structurally related to Mrs2p"
/codon_start=1
/product="Mfm1p"
/protein_id="NP_015265.1"
/db_xref="GeneID:856045"
/db_xref="SGD:S000005981"
/translation="MRAFPRVLPFRHQRSYNNILLRTVRLFGSSLSSFDFSRQMPKVD
PDNTAAMLLQKNLIQRNNMLYGYGSGTIRCTLLDSTGRAKSPLVEIKREDLVSKHGLL
PRDLRKIEKSRKNDLVPSLLVRENSILISLLTVKALIKPDMVIIFDSAGSGITLNSEA
HKDFINDMKLRLKNQETSELNSDPLPYEFRALETIFISALSNLTSEMKVLLTICKGVL
QDLEFSITRDKLRFLLGQNKKLSSFNKKAVLVKDMLDDLLEQDDMLCDMYLTDKKAGK
IRVQDDHTEIEMLLETYHNYVDEIVQKSESAISDVKTTEEIINIILDSNRNELMLLGI
RYAIGMLSLGGALFLGSIYGMNLESFIEESNYAYLTVTILGLISTVWLYAKGIRHLHK
LQRMTLLSKIKTDSVHELLKK"
gene complement(435893..435964)
/locus_tag="YNCP0007C"
/db_xref="GeneID:856046"
tRNA complement(435893..435964)
/locus_tag="YNCP0007C"
/product="tRNA-Cys"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Cysteine tRNA (tRNA-Cys), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:856046"
/db_xref="SGD:S000006528"
repeat_region 436068..436249
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007196"
repeat_region complement(436250..436587)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007185"
repeat_region 436594..436731
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007197"
mobile_element complement(436890..443112)
/note="YPLCTy4-1; Ty4 element, LTR retrotransposon of the
Copia (Pseudoviridae) group; contains co-transcribed genes
TYA Gag and TYB Pol, encoding proteins involved in
structure and function of virus-like particles, flanked by
two direct repeats"
/mobile_element_type="retrotransposon:YPLCTy4-1"
/db_xref="SGD:S000007188"
repeat_region complement(436890..437260)
/note="Ty4 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007186"
gene complement(<437333..>442742)
/locus_tag="YPL060C-A"
/db_xref="GeneID:856047"
mRNA complement(<437333..>442742)
/locus_tag="YPL060C-A"
/product="gag-pol fusion protein"
/transcript_id="NM_001305015.2"
/db_xref="GeneID:856047"
CDS complement(join(437333..439427,439429..442742))
/locus_tag="YPL060C-A"
/EC_number="2.7.7.7"
/EC_number="2.7.7.49"
/EC_number="3.1.26.4"
/ribosomal_slippage
/note="TyA Gag protein; the main structural constituent of
virus-like particles (VLPs); Gag processing produces
capsid proteins"
/codon_start=1
/product="gag-pol fusion protein"
/protein_id="NP_001291944.1"
/db_xref="GeneID:856047"
/db_xref="SGD:S000007388"
/translation="MATPVRDETRNVIDDNISARIQSKVKTNDTVRQTPSSLRKVSIK
DEQVKQYQRNLNRFKTILNGLKAEEEKLSETDDIQMLAEKLLKLGETIDKVENRIVDL
VEKIQLLETNENNNILHEHIDATGTYYLFDTLTSTNKRFYPKDCVFDYRTNNVENIPI
LLNNFKKFIKKYQFDDVFENDIIEIDPRENEILCKIIKEGLGESLDIMNTNTTDIFRI
IDGLKNKYRSLHGRDVRIRAWEKVLVDTTCRNSALLMNKLQKLVLMEKWIFSKCCQDC
PNLKDYLQEAIMGTLHESLRNSVKQRLYNIPHNVGINHEEFLINTVIETVIDLSPIAD
DQIENSCMYCKSVFHCSINCKKKPNRELRPDSTNFSKTYYLQGAQRQQQLKSSAKEQK
SWNKTQKKSNKVYNSKKLVIIDTGSGVNITNDKTLLHNYEDSNRSTRFFGIGKNSSVS
VKGYGYIKIKNGHNNTDNKCLLTYYVPEEESTIISCYDLAKKTKMVLSRKYTRLGNKI
IKIKTKIVNGVIHVKMNELIERPSDDSKINAIKPTSSPGFKLNKRSITLEDAHKRMGH
TGIQQIENSIKHNHYEESLDLIKEPNEFWCQTCKISKATKRNHYTGSMNNHSTDHEPG
SSWCMDIFGPVSSSNADTKRYMLIMVDNNTRYCMTSTHFNKNAETILAQIRKNIQYVE
TQFDRKVREINSDRGTEFTNDQIEEYFISKGIHHILTSTQDHAANGRAERYIRTIVTD
ATTLLRQSNLRVKFWEYAVTSATNIRNCLEHKSTGKLPLKAISRQPVTVRLMSFLPFG
EKGIIWNHNHKKLKPSGLPSIILCKDPNSYGYKFFIPSKNKIVTSDNYTIPNYTMDGR
VRNTQNIYKSHQFSSHNDNEEDQIETVTNLCEALENYEDDNKPITRLEDLFTEEELSQ
IDSNAKYPSPSNNLEGDLDYVFSDVEESGDYDVESELSTTNTSISTDKNKILSNKDFN
SELASTEISISEIDKKGLINTSHIDEDKYDEKVHRIPSIIQEKLVGSKNTIKINDENR
ISDRIRSKNIGSILNTGLSRCVDITDESITNKDESMHNAKPELIQEQFNKTNHETSFP
KEGSIGTKCKIPKYRQWISLKTGDTSLPIKTLESINNHHSNDYSTNKVEKFEKENHHP
PPIEDIVDMSDQTDMESNCQDGNNLKELKVTDKNVPTDNGTNVSPRLEQNIEASGSPV
QTVNKSAFLNKEFSSLNMKRKRKRHDKNNSLTSYELERDKKRSKRNRVKLIPDNMETV
SAQKIRAIYYNEAISKNPDLKEKHEYKQAYHKELQNLKDMKVFDVDVKYSRSEIPDNL
IVPTNTIFTKKRNGIYKARIVCRGDTQSPDTYSVITTESLNHNHIKIFLMIANNRNMF
MKTLDINHAFLYAKLEEEIYIPHPHDRRCVVKLNKALYGLKQSPKEWNDHLRQYLNGI
GLKDNSYTPGLYQTEDKNLMIAVYVDDCVIAASNEQRLDEFINKLKSNFELKITGTLI
DDVLDTDILGMDLVYNKRLGTIDLTLKSFINRMDKKYNEELKKIRKSSIPHMSTYKID
PKKDVLQMSEEEFRQGVLKLQQLLGELNYVRHKCRYDINFAVKKVARLVNYPHERVFY
MIYKIIQYLVRYKDIGIHYDRDCNKDKKVIAITDASVGSEYDAQSRIGVILWYGMNIF
NVYSNKSTNRCVSSTEAELHAIYEGYADSETLKVTLKELGEGDNNDIVMITDSKPAIQ
GLNRSYQQPKEKFTWIKTEIIKEKIKEKSIKLLKITGKGNIADLLTKPVSASDFKRFI
QVLKNKITSQDILASTDY"
repeat_region complement(442742..443112)
/note="Ty4 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007187"
gene <444579..>445031
/gene="GRX5"
/locus_tag="YPL059W"
/db_xref="GeneID:856048"
mRNA <444579..>445031
/gene="GRX5"
/locus_tag="YPL059W"
/product="monothiol glutaredoxin GRX5"
/transcript_id="NM_001183873.1"
/db_xref="GeneID:856048"
CDS 444579..445031
/gene="GRX5"
/locus_tag="YPL059W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:11950925|PMID:15456753]"
/experiment="EXISTENCE:direct assay:GO:0015036 disulfide
oxidoreductase activity [PMID:12730244]"
/experiment="EXISTENCE:direct assay:GO:0051537 2 iron, 2
sulfur cluster binding [PMID:23615440]"
/experiment="EXISTENCE:genetic interaction:GO:0016226
iron-sulfur cluster assembly [PMID:11950925]"
/experiment="EXISTENCE:mutant phenotype:GO:0006970
response to osmotic stress [PMID:10567543]"
/experiment="EXISTENCE:mutant phenotype:GO:0015036
disulfide oxidoreductase activity [PMID:10567543]"
/experiment="EXISTENCE:mutant phenotype:GO:0016226
iron-sulfur cluster assembly
[PMID:12970193|PMID:11950925]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress [PMID:10567543]"
/experiment="EXISTENCE:mutant phenotype:GO:0044571
[2Fe-2S] cluster assembly [PMID:23615440]"
/experiment="EXISTENCE:mutant phenotype:GO:0044572
[4Fe-4S] cluster assembly [PMID:23615440]"
/note="Glutathione-dependent oxidoreductase; mitochondrial
matrix protein and subunit of an iron-sulfur assembly
complex with Bol1p acting at a late stage in iron-sulfur
center biogenesis; acts as a central hub in iron-sulfur
cluster intermediate trafficking within mitochondria and
export to the cytoplasm; hydroperoxide and
superoxide-radical responsive; monothiol glutaredoxin
subfamily member along with Grx3p and Grx4p"
/codon_start=1
/product="monothiol glutaredoxin GRX5"
/protein_id="NP_015266.1"
/db_xref="GeneID:856048"
/db_xref="SGD:S000005980"
/translation="MFLPKFNPIRSFSPILRAKTLLRYQNRMYLSTEIRKAIEDAIES
APVVLFMKGTPEFPKCGFSRATIGLLGNQGVDPAKFAAYNVLEDPELREGIKEFSEWP
TIPQLYVNKEFIGGCDVITSMARSGELADLLEEAQALVPEEEEETKDR"
gene complement(<445842..>450377)
/gene="PDR12"
/locus_tag="YPL058C"
/db_xref="GeneID:856049"
mRNA complement(<445842..>450377)
/gene="PDR12"
/locus_tag="YPL058C"
/product="ATP-binding cassette multidrug transporter
PDR12"
/transcript_id="NM_001183872.1"
/db_xref="GeneID:856049"
CDS complement(445842..450377)
/gene="PDR12"
/locus_tag="YPL058C"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:9687494]"
/experiment="EXISTENCE:direct assay:GO:0015849 organic
acid transport [PMID:10419965]"
/experiment="EXISTENCE:direct assay:GO:0022857
transmembrane transporter activity [PMID:10419965]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="Plasma membrane ATP-binding cassette (ABC)
transporter; weak-acid-inducible multidrug transporter
required for weak organic acid resistance; induced by
sorbate and benzoate and regulated by War1p; mutants
exhibit sorbate hypersensitivity"
/codon_start=1
/product="ATP-binding cassette multidrug transporter
PDR12"
/protein_id="NP_015267.1"
/db_xref="GeneID:856049"
/db_xref="SGD:S000005979"
/translation="MSSTDEHIEKDISSRSNHDDDYANSVQSYAASEGQVDNEDLAAT
SQLSRHLSNILSNEEGIERLESMARVISHKTKKEMDSFEINDLDFDLRSLLHYLRSRQ
LEQGIEPGDSGIAFKNLTAVGVDASAAYGPSVEEMFRNIASIPAHLISKFTKKSDVPL
RNIIQNCTGVVESGEMLFVVGRPGAGCSTFLKCLSGETSELVDVQGEFSYDGLDQSEM
MSKYKGYVIYCPELDFHFPKITVKETIDFALKCKTPRVRIDKMTRKQYVDNIRDMWCT
VFGLRHTYATKVGNDFVRGVSGGERKRVSLVEAQAMNASIYSWDNATRGLDASTALEF
AQAIRTATNMVNNSAIVAIYQAGENIYELFDKTTVLYNGRQIYFGPADKAVGYFQRMG
WVKPNRMTSAEFLTSVTVDFENRTLDIKPGYEDKVPKSSSEFEEYWLNSEDYQELLRT
YDDYQSRHPVNETRDRLDVAKKQRLQQGQRENSQYVVNYWTQVYYCMIRGFQRVKGDS
TYTKVYLSSFLIKALIIGSMFHKIDDKSQSTTAGAYSRGGMLFYVLLFASVTSLAEIG
NSFSSRPVIVKHKSYSMYHLSAESLQEIITEFPTKFVAIVILCLITYWIPFMKYEAGA
FFQYILYLLTVQQCTSFIFKFVATMSKSGVDAHAVGGLWVLMLCVYAGFVLPIGEMHH
WIRWLHFINPLTYAFESLVSTEFHHREMLCSALVPSGPGYEGISIANQVCDAAGAVKG
NLYVSGDSYILHQYHFAYKHAWRNWGVNIVWTFGYIVFNVILSEYLKPVEGGGDLLLY
KRGHMPELGTENADARTASREEMMEALNGPNVDLEKVIAEKDVFTWNHLDYTIPYDGA
TRKLLSDVFGYVKPGKMTALMGESGAGKTTLLNVLAQRINMGVITGDMLVNAKPLPAS
FNRSCGYVAQADNHMAELSVRESLRFAAELRQQSSVPLEEKYEYVEKIITLLGMQNYA
EALVGKTGRGLNVEQRKKLSIGVELVAKPSLLLFLDEPTSGLDSQSAWSIVQFMRALA
DSGQSILCTIHQPSATLFEQFDRLLLLKKGGKMVYFGDIGPNSETLLKYFERQSGMKC
GVSENPAEYILNCIGAGATASVNSDWHDLWLASPECAAARAEVEELHRTLPGRAVNDD
PELATRFAASYMTQIKCVLRRTALQFWRSPVYIRAKFFECVACALFVGLSYVGVNHSV
GGAIEAFSSIFMLLLIALAMINQLHVFAYDSRELYEVREAASNTFHWSVLLLCHAAVE
NFWSTLCQFMCFICYYWPAQFSGRASHAGFFFFFYVLIFPLYFVTYGLWILYMSPDVP
SASMINSNLFAAMLLFCGILQPREKMPAFWRRLMYNVSPFTYVVQALVTPLVHNKKVV
CNPHEYNIMDPPSGKTCGEFLSTYMDNNTGYLVNPTATENCQYCPYTVQDQVVAKYNV
KWDHRWRNFGFMWAYICFNIAAMLICYYVVRVKVWSLKSVLNFKKWFNGPRKERHEKD
TNIFQTVPGDENKITKK"
gene complement(<451909..>453057)
/gene="SUR1"
/locus_tag="YPL057C"
/gene_synonym="BCL21; CSG1; LPE15"
/db_xref="GeneID:856050"
mRNA complement(<451909..>453057)
/gene="SUR1"
/locus_tag="YPL057C"
/gene_synonym="BCL21; CSG1; LPE15"
/product="mannosylinositol phosphorylceramide synthase
catalytic subunit SUR1"
/transcript_id="NM_001183871.1"
/db_xref="GeneID:856050"
CDS complement(451909..453057)
/gene="SUR1"
/locus_tag="YPL057C"
/gene_synonym="BCL21; CSG1; LPE15"
/EC_number="2.4.1.370"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:19001347]"
/experiment="EXISTENCE:mutant phenotype:GO:0006675
mannosyl-inositol phosphorylceramide metabolic process
[PMID:9323360]"
/experiment="EXISTENCE:mutant phenotype:GO:0006688
glycosphingolipid biosynthetic process [PMID:9323360]"
/experiment="EXISTENCE:mutant phenotype:GO:0030148
sphingolipid biosynthetic process [PMID:12954640]"
/experiment="EXISTENCE:mutant phenotype:GO:0103064
inositol phosphorylceramide mannosyltransferase activity
[PMID:12954640]"
/note="Mannosylinositol phosphorylceramide (MIPC) synthase
catalytic subunit; forms a complex with regulatory subunit
Csg2p; function in sphingolipid biosynthesis is
overlapping with that of Csh1p; SUR1 has a paralog, CSH1,
that arose from the whole genome duplication"
/codon_start=1
/product="mannosylinositol phosphorylceramide synthase
catalytic subunit SUR1"
/protein_id="NP_015268.1"
/db_xref="GeneID:856050"
/db_xref="SGD:S000005978"
/translation="MRKELKYLICFNILLLLSIIYYTFDLLTLCIDDTVKDAILEEDL
NPDAPPKPQLIPKIIHQTYKTEDIPEHWKEGRQKCLDLHPDYKYILWTDEMAYEFIKE
EYPWFLDTFENYKYPIERADAIRYFILSHYGGVYIDLDDGCERKLDPLLAFPAFLRKT
SPLGVSNDVMGSVPRHPFFLKALKSLKHYDKYWFIPYMTIMGSTGPLFLSVIWKQYKR
WRIPKNGTVRILQPAYYKMHSYSFFSITKGSSWHLDDAKLMKALENHILSCVVTGFIF
GFFILYGEFTFYCWLCSKNFSNLTKNWKLNAIKVRFVTILNSLGLRLKLSKSTSDTAS
ATLLARQQKRLRKDSNTNIVLLKSSRKSDVYDLEKNDSSKYSLGNNSS"
gene complement(<453430..>453735)
/gene="LCL1"
/locus_tag="YPL056C"
/db_xref="GeneID:856051"
mRNA complement(<453430..>453735)
/gene="LCL1"
/locus_tag="YPL056C"
/product="Lcl1p"
/transcript_id="NM_001183870.1"
/db_xref="GeneID:856051"
CDS complement(453430..453735)
/gene="LCL1"
/locus_tag="YPL056C"
/note="hypothetical protein; deletion mutant is
fluconazole resistant and has long chronological lifespan"
/codon_start=1
/product="Lcl1p"
/protein_id="NP_015269.1"
/db_xref="GeneID:856051"
/db_xref="SGD:S000005977"
/translation="MKNAALCEALPLLATCSHEIPPTPHTVCFVFPPALLLSPSKLTL
LNSRRVASRCVIIIDPRLLRLFSCSRPQQLPRDKNQSFAKPSFSFFFFLLTSLLSPF"
gene complement(<453992..>454990)
/gene="LGE1"
/locus_tag="YPL055C"
/db_xref="GeneID:856052"
mRNA complement(<453992..>454990)
/gene="LGE1"
/locus_tag="YPL055C"
/product="Lge1p"
/transcript_id="NM_001183869.1"
/db_xref="GeneID:856052"
CDS complement(453992..454990)
/gene="LGE1"
/locus_tag="YPL055C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:15528187|PMID:14562095|PMID:32214243]"
/experiment="EXISTENCE:direct assay:GO:0140693 molecular
condensate scaffold activity [PMID:32214243]"
/experiment="EXISTENCE:mutant phenotype:GO:0006279
premeiotic DNA replication [PMID:18069899]"
/experiment="EXISTENCE:mutant phenotype:GO:0008361
regulation of cell size [PMID:12089449]"
/experiment="EXISTENCE:mutant phenotype:GO:0031509
subtelomeric heterochromatin formation [PMID:37431950]"
/experiment="EXISTENCE:physical interaction:GO:0140693
molecular condensate scaffold activity [PMID:32214243]"
/note="Protein that facilitates co-transcriptional histone
H2B ubiquitination; scaffold protein that promotes
oligomerization of E3 ligase Bre1p; contains an
intrinsically disordered region that phase separates, to
form a core encapsulated by Bre1p that recruits
Ub-conjugating enzyme Rad6p, elevating catalytic activity
along gene bodies; null mutant forms abnormally large
cells, and homozygous null mutant displays delayed
premeiotic DNA synthesis and reduced efficiency of meiotic
nuclear division"
/codon_start=1
/product="Lge1p"
/protein_id="NP_015270.1"
/db_xref="GeneID:856052"
/db_xref="SGD:S000005976"
/translation="MSGYTGNNYSRYSSTPPRQRGGYHHARRSRGGAGGSYYRGGNAS
YGARYNSDYEQPPQEGDLRQTGAYYRNGYTDTRPYYSANSRHYQAQPSPRYNNGTNSY
HLPQRGNSQDTNGRTTSASQEDNDEKRVKSRYRNMQADHPRQQPMSVGSTSSRNGSSG
NSSTSSTSNGLPPPPSVSSITNNRSYHSSAYPYSSSHTYNNYHHRETPPPPPSNGYYA
KGYPVHVPENRSNSDGSSSSVVKKKRILDMKDSPFIYLTDFDKNVKKTNNTESECEKA
REVFKESDSIDSALEELNLKINSNELELRLLNNQCDKHALNIQLTQEKLDSLLLMQ"
gene <455759..>456664
/gene="LEE1"
/locus_tag="YPL054W"
/db_xref="GeneID:856053"
mRNA <455759..>456664
/gene="LEE1"
/locus_tag="YPL054W"
/product="Lee1p"
/transcript_id="NM_001183868.1"
/db_xref="GeneID:856053"
CDS 455759..456664
/gene="LEE1"
/locus_tag="YPL054W"
/note="Zinc-finger hypothetical protein"
/codon_start=1
/product="Lee1p"
/protein_id="NP_015271.1"
/db_xref="GeneID:856053"
/db_xref="SGD:S000005975"
/translation="MDAFENMSVSNHPGGNARRNSQSANEMLASQIQDFQNIPRSFND
SNANVNLSKNCTVGNQLPFSSRQQKIIMEHLLITKNNSQQQKDYSHVPCKFFKMGNCQ
AGSSCPFSHSPDIISSANNLPCKYFAKGNCKFGNKCVNAHVLPNGFKMNSKEPIDITP
PSQNNYLSHARSASFSTYTSPPLSAQTEFSHSASNANYFSSQYLMYSPQKSPEALYTE
FFSPPSSSSSYINYSYNNSNINAYSPVSSSSSNIWQEQGQTTLSNPSVNQNLRYRTGP
AIQEESDNEIEDLLIHNFNSRYCHE"
rep_origin 456563..456810
/note="ARS1633; Autonomously Replicating Sequence"
/db_xref="SGD:S000119038"
gene complement(<457118..>458458)
/gene="KTR6"
/locus_tag="YPL053C"
/gene_synonym="MNN6"
/db_xref="GeneID:856054"
mRNA complement(<457118..>458458)
/gene="KTR6"
/locus_tag="YPL053C"
/gene_synonym="MNN6"
/product="putative mannosyltransferase"
/transcript_id="NM_001183867.1"
/db_xref="GeneID:856054"
CDS complement(457118..458458)
/gene="KTR6"
/locus_tag="YPL053C"
/gene_synonym="MNN6"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:30842360]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:9218445]"
/experiment="EXISTENCE:mutant phenotype:GO:0000031
mannosylphosphate transferase activity [PMID:9218445]"
/experiment="EXISTENCE:mutant phenotype:GO:0006487 protein
N-linked glycosylation [PMID:9218445]"
/experiment="EXISTENCE:mutant phenotype:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:30842360]"
/experiment="EXISTENCE:mutant phenotype:GO:0031505
fungal-type cell wall organization [PMID:9090056]"
/note="Probable mannosylphosphate transferase; involved in
the synthesis of core oligosaccharides in protein
glycosylation pathway; facilitates the exit of Mnn4p from
the ER via COPII-coated vesicles; member of the KRE2/MNT1
mannosyltransferase family; KTR6 has a paralog, KRE2, that
arose from the whole genome duplication"
/codon_start=1
/product="putative mannosyltransferase"
/protein_id="NP_015272.1"
/db_xref="GeneID:856054"
/db_xref="SGD:S000005974"
/translation="MHVLLSKKIARFLLISFVFVLALMVTINHPKTKQMSEQYVTPYL
PKSLQPIAKISAEEQRRIQSEQEEAELKQSLEGEAIRNATVNAIKEKIKSYGGNETTL
GFMVPSYINHRGSPPKACFVSLITERDSMTQILQSIDEVQVKFNKNFAYPWVFISQGE
LDGMKQEMIRQAITDSMNGDPELINIKFAEIPADEWVYPEWIDENKAAESLISLANVP
DGDSRAVRYQARYFAGFFWRHPVLDEFDWYWRVDPGIKLYCDIDHDLFRWMQDEGKVF
GFTLSMSEAKEANEKIWDVTKKFAKDFPKFISENNFKSFITKKDSEDFNNCEFTSNFE
IGNLNFYRSPAYRKFFNYIDEEGGIFYWKWSDSIIHTIGLSMLLPKDKIHFFENIGFH
YDKYNNCPLNDDIWNQYNCNCDQGNDFTFRSGSCGGHYFDIMKKDKPEGWDRLP"
gene <458799..>459678
/gene="OAZ1"
/locus_tag="YPL052W"
/gene_synonym="YPL052W-A"
/db_xref="GeneID:856055"
mRNA <458799..>459678
/gene="OAZ1"
/locus_tag="YPL052W"
/gene_synonym="YPL052W-A"
/product="Oaz1p"
/transcript_id="NM_001183866.4"
/db_xref="GeneID:856055"
CDS join(458799..459005,459007..459678)
/gene="OAZ1"
/locus_tag="YPL052W"
/gene_synonym="YPL052W-A"
/experiment="EXISTENCE:curator inference:GO:0005737
cytoplasm [PMID:15538383]"
/experiment="EXISTENCE:direct assay:GO:0008073 ornithine
decarboxylase inhibitor activity [PMID:15538383]"
/experiment="EXISTENCE:direct assay:GO:0061136 regulation
of proteasomal protein catabolic process [PMID:18089576]"
/experiment="EXISTENCE:mutant phenotype:GO:2001125
negative regulation of translational frameshifting
[PMID:21900894]"
/ribosomal_slippage
/note="Regulator of ornithine decarboxylase Spe1p;
antizyme that binds to Spe1p to stimulate
ubiquitin-independent degradation by the proteasome;
binding of polyamines to nascent Oaz1p during translation
stimulates +1 ribosomal frameshifting, allowing
translation of full-length Oaz1p"
/codon_start=1
/product="Oaz1p"
/protein_id="NP_015273.2"
/db_xref="GeneID:856055"
/db_xref="SGD:S000005973"
/translation="MYEVIQKRKTKIINVLQSPELMRLIEDPSNLGISLHFPVSSLLK
SNKCTPMPKLSTYSLASGGFKDWCADIPLDVPPEIDIIDFYWDVILCMESQFILDYNV
PSKNKGNNQKSVAKLLKNKLVNDMKTTLKRLIYNENTKQYKNNNSHDGYNWRKLGSQY
FILYLPLFTQELIWCKLNENYFHVVLPSLLNSRNVHDNHSTYINKDWLLALLELTSNL
NQNFKFEYMKLRLYILRDDLINNGLDLLKNLNWVGGKLIKNEDREVLLNSTDLATDSI
SHLLGDENFVILEFEC"
gene <459963..>460559
/gene="ARL3"
/locus_tag="YPL051W"
/db_xref="GeneID:856056"
mRNA <459963..>460559
/gene="ARL3"
/locus_tag="YPL051W"
/product="Arf family GTPase ARL3"
/transcript_id="NM_001183865.1"
/db_xref="GeneID:856056"
CDS 459963..460559
/gene="ARL3"
/locus_tag="YPL051W"
/experiment="EXISTENCE:direct assay:GO:0003924 GTPase
activity [PMID:9920936]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:15077114|PMID:15077113]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0042175 nuclear
outer membrane-endoplasmic reticulum membrane network
[PMID:9920936]"
/experiment="EXISTENCE:mutant phenotype:GO:0006886
intracellular protein transport [PMID:9920936]"
/experiment="EXISTENCE:mutant phenotype:GO:0034067 protein
localization to Golgi apparatus
[PMID:12620189|PMID:26966233]"
/experiment="EXISTENCE:mutant phenotype:GO:0034976
response to endoplasmic reticulum stress [PMID:26966233]"
/experiment="EXISTENCE:mutant phenotype:GO:0043001 Golgi
to plasma membrane protein transport [PMID:16926193]"
/note="ARF-like small GTPase of the RAS superfamily;
required for recruitment of Arl1p, a GTPase that regulates
membrane traffic, to the Golgi apparatus; NatC-catalyzed
N-terminal acetylation regulates Golgi membrane
association mediated by interaction with membrane
receptor, Sys1p; similar to ADP-ribosylation factor and
orthologous to mammalian ARFRP1"
/codon_start=1
/product="Arf family GTPase ARL3"
/protein_id="NP_015274.1"
/db_xref="GeneID:856056"
/db_xref="SGD:S000005972"
/translation="MFHLVKGLYNNWNKKEQYSILILGLDNAGKTTFLETLKKEYSLA
FKALEKIQPTVGQNVATIPVDSKQILKFWDVGGQESLRSMWSEYYSLCHGIIFIVDSS
DRERLDECSTTLQSVVMDEEIEGVPILMLANKQDRQDRMEVQDIKEVFNKIAEHISAR
DSRVLPISALTGEGVKDAIEWMIVRLERNKKSRPPIYK"
gene complement(<460779..>461966)
/gene="MNN9"
/locus_tag="YPL050C"
/db_xref="GeneID:856057"
mRNA complement(<460779..>461966)
/gene="MNN9"
/locus_tag="YPL050C"
/product="mannosyltransferase complex subunit MNN9"
/transcript_id="NM_001183864.1"
/db_xref="GeneID:856057"
CDS complement(460779..461966)
/gene="MNN9"
/locus_tag="YPL050C"
/experiment="EXISTENCE:direct assay:GO:0000009
alpha-1,6-mannosyltransferase activity [PMID:19249370]"
/experiment="EXISTENCE:direct assay:GO:0000136 mannan
polymerase complex [PMID:9430634]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005801 cis-Golgi
network [PMID:9430634]"
/experiment="EXISTENCE:direct assay:GO:0006487 protein
N-linked glycosylation [PMID:9430634|PMID:19249370]"
/note="Subunit of Golgi mannosyltransferase complex; this
complex mediates elongation of the polysaccharide mannan
backbone; forms a separate complex with Van1p that is also
involved in backbone elongation; this complex also
contains Anp1p, Mnn10p, Mnn11p, and Hoc1p"
/codon_start=1
/product="mannosyltransferase complex subunit MNN9"
/protein_id="NP_015275.1"
/db_xref="GeneID:856057"
/db_xref="SGD:S000005971"
/translation="MSLSLVSYRLRKNPWVNIFLPVLAIFLIYIIFFQRDQSLLGLNG
QSISQHKWAHEKENTFYFPFTKKYKMPKYSYKKKSGWLFNDHVEDIIPEGHIAHYDLN
KLHSTSEAAVNKEHILILTPMQTFHQQYWDNLLQLNYPRELIELGFITPRTATGDLAL
KKLENAIKKVQTDKKTQRFSKITILRQNSQSFDKLMEKERHALDVQKERRAAMALARN
ELLFSTIGPHTSWVLWLDADIIETPPSLIQDMTKHNKAILAANIYQRFYDEEKKQPSI
RPYDFNNWQESDTGLEIASQMGDDEIIVEGYAEIATYRPLMAHFYDANGVPGEEMALD
GVGGGCTLVKAEVHRDGAMFPNFPFYHLIETEGFAKMAKRLNYDVFGLPNYLVYHIEE
ENH"
gene complement(<462481..>463839)
/gene="DIG1"
/locus_tag="YPL049C"
/gene_synonym="RST1"
/db_xref="GeneID:856058"
mRNA complement(<462481..>463839)
/gene="DIG1"
/locus_tag="YPL049C"
/gene_synonym="RST1"
/product="Dig1p"
/transcript_id="NM_001183863.1"
/db_xref="GeneID:856058"
CDS complement(462481..463839)
/gene="DIG1"
/locus_tag="YPL049C"
/gene_synonym="RST1"
/experiment="EXISTENCE:direct assay:GO:0003714
transcription corepressor activity [PMID:10825185]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:8918885|PMID:9094309]"
/experiment="EXISTENCE:direct assay:GO:1990526
Ste12p-Dig1p-Dig2p complex [PMID:16782869]"
/experiment="EXISTENCE:direct assay:GO:1990527
Tec1p-Ste12p-Dig1p complex [PMID:16782869]"
/experiment="EXISTENCE:genetic interaction:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:9860980|PMID:9094309]"
/experiment="EXISTENCE:genetic interaction:GO:0071444
cellular response to pheromone
[PMID:9860980|PMID:9094309]"
/experiment="EXISTENCE:genetic interaction:GO:2000218
negative regulation of invasive growth in response to
glucose limitation [PMID:8918885|PMID:9094309]"
/experiment="EXISTENCE:genetic interaction:GO:2000221
negative regulation of pseudohyphal growth
[PMID:8918885|PMID:9094309]"
/note="MAP kinase-responsive inhibitor of the Ste12p
transcription factor; involved in the regulation of
mating-specific genes and the invasive growth pathway;
related regulators Dig1p and Dig2p bind to Ste12p; DIG1
has a paralog, DIG2, that arose from the whole genome
duplication"
/codon_start=1
/product="Dig1p"
/protein_id="NP_015276.1"
/db_xref="GeneID:856058"
/db_xref="SGD:S000005970"
/translation="MAVSARLRTTAEDTSIAKSTQDPIGDTEISVANAKGSSDSNIKN
SPGGNSVGQESELEHVPEEDDSGDKEADHEDSETATAKKRKAQPLKNPKKSLKRGRVP
APLNLSDSNTNTHGGNIKDGNLASSNSAHFPPVANQNVKSAPAQVTQHSKFQPRVQYL
GKASSRQSIQVNNSSNSYGKPHMPSAGIMSAMNPYMPMNRYIMSPYYNPYGIPPPHML
NKPIMTPYVSYPYPMGPRTSIPYAMQGGNARPYEENEYSASNYRNKRVNDSYDSPLSG
TASTGKTRRSEEGSRNSSVGSSANAGPTQQRADLRPADMIPAEEYHFERDALLSANTK
ARSASTSTSTSTSTNRDRSSWHEAEPNKDEEEGTDLAIEDGAVPTPTFTTFQRTSQPQ
QQSPSLLQGEIRLSSHIFAFEFPLSSSNVDKKMFMSICNKVWNESKELTKKSSSHHRT
GK"
gene <464401..>465648
/gene="CAM1"
/locus_tag="YPL048W"
/gene_synonym="TEF3"
/db_xref="GeneID:856059"
mRNA <464401..>465648
/gene="CAM1"
/locus_tag="YPL048W"
/gene_synonym="TEF3"
/product="translation elongation factor EF1B gamma"
/transcript_id="NM_001183862.1"
/db_xref="GeneID:856059"
CDS 464401..465648
/gene="CAM1"
/locus_tag="YPL048W"
/gene_synonym="TEF3"
/experiment="EXISTENCE:direct assay:GO:0001046 core
promoter sequence-specific DNA binding [PMID:12824466]"
/experiment="EXISTENCE:direct assay:GO:0005085
guanyl-nucleotide exchange factor activity [PMID:3528160]"
/experiment="EXISTENCE:direct assay:GO:0005509 calcium ion
binding [PMID:8465602]"
/experiment="EXISTENCE:direct assay:GO:0005543
phospholipid binding [PMID:8465602]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12824466]"
/experiment="EXISTENCE:direct assay:GO:0006414
translational elongation [PMID:3528160]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405]"
/experiment="EXISTENCE:genetic interaction:GO:0006449
regulation of translational termination [PMID:19545407]"
/experiment="EXISTENCE:mutant phenotype:GO:0003700
DNA-binding transcription factor activity [PMID:12824466]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:12824466]"
/note="One of two isoforms of the gamma subunit of eEF1B;
stimulates the release of GDP from eEF1A (Tef1p/Tef2p)
post association with the ribosomal complex with
eEF1Balpha subunit; nuclear protein required for
transcription of MXR1; binds the MXR1 promoter in the
presence of other nuclear factors; binds calcium and
phospholipids"
/codon_start=1
/product="translation elongation factor EF1B gamma"
/protein_id="NP_015277.1"
/db_xref="GeneID:856059"
/db_xref="SGD:S000005969"
/translation="MSQGTLYANFRIRTWVPRGLVKALKLDVKVVTPDAAAEQFARDF
PLKKVPAFVGPKGYKLTEAMAINYYLVKLSQDDKMKTQLLGADDDLNAQAQIIRWQSL
ANSDLCIQIANTIVPLKGGAPYNKKSVDSAMDAVDKIVDIFENRLKNYTYLATENISL
ADLVAASIFTRYFESLFGTEWRAQHPAIVRWFNTVRASPFLKDEYKDFKFADKPLSPP
QKKKEKKAPAAAPAASKKKEEAKPAATETETSSKKPKHPLELLGKSTFVLDDWKRKYS
NEDTRPVALPWFWEHYNPEEYSLWKVTYKYNDELTLTFMSNNLVGGFFNRLSASTKYM
FGCLVVYGENNNNGIVGAVMVRGQDYVPAFDVAPDWESYDYAKLDPTNDDDKEFINNM
WAWDKPVSVNGEPKEIVDGKVLK"
gene <465962..>466261
/gene="SGF11"
/locus_tag="YPL047W"
/db_xref="GeneID:856060"
mRNA <465962..>466261
/gene="SGF11"
/locus_tag="YPL047W"
/product="SAGA histone acetyltransferase complex subunit
SGF11"
/transcript_id="NM_001183861.1"
/db_xref="GeneID:856060"
CDS 465962..466261
/gene="SGF11"
/locus_tag="YPL047W"
/experiment="EXISTENCE:direct assay:GO:0000124 SAGA
complex [PMID:15282323]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008047 enzyme
activator activity [PMID:18488019]"
/experiment="EXISTENCE:direct assay:GO:0071819 DUBm
complex [PMID:20395473]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:15282323]"
/note="Integral subunit of SAGA histone acetyltransferase
complex; regulates transcription of a subset of
SAGA-regulated genes, required for the Ubp8p association
with SAGA and for H2B deubiquitylation"
/codon_start=1
/product="SAGA histone acetyltransferase complex subunit
SGF11"
/protein_id="NP_015278.1"
/db_xref="GeneID:856060"
/db_xref="SGD:S000005968"
/translation="MTEETITIDSISNGILNNLLTTLIQDIVARETTQQQLLKTRYPD
LRSYYFDPNGSLDINGLQKQQESSQYIHCENCGRDVSANRLAAHLQRCLSRGARR"
gene complement(<466644..>466943)
/gene="ELC1"
/locus_tag="YPL046C"
/db_xref="GeneID:856061"
mRNA complement(<466644..>466943)
/gene="ELC1"
/locus_tag="YPL046C"
/product="elongin C"
/transcript_id="NM_001183860.1"
/db_xref="GeneID:856061"
CDS complement(466644..466943)
/gene="ELC1"
/locus_tag="YPL046C"
/experiment="EXISTENCE:direct assay:GO:0000113
nucleotide-excision repair factor 4 complex
[PMID:15226437]"
/experiment="EXISTENCE:direct assay:GO:0004842
ubiquitin-protein transferase activity [PMID:16675952]"
/experiment="EXISTENCE:direct assay:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:16675952]"
/experiment="EXISTENCE:direct assay:GO:0034644 cellular
response to UV [PMID:16675952]"
/experiment="EXISTENCE:mutant phenotype:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:16705154]"
/experiment="EXISTENCE:mutant phenotype:GO:0031463
Cul3-RING ubiquitin ligase complex [PMID:17296727]"
/experiment="EXISTENCE:mutant phenotype:GO:0070911 global
genome nucleotide-excision repair [PMID:18817898]"
/experiment="EXISTENCE:physical interaction:GO:0031463
Cul3-RING ubiquitin ligase complex [PMID:16675952]"
/experiment="EXISTENCE:physical interaction:GO:0070449
elongin complex [PMID:10430890|PMID:10753924]"
/note="Elongin C, conserved among eukaryotes; forms a
complex with Cul3p that polyubiquitylates
monoubiquitylated RNA polymerase II to trigger its
proteolysis; plays a role in global genomic repair"
/codon_start=1
/product="elongin C"
/protein_id="NP_015279.1"
/db_xref="GeneID:856061"
/db_xref="SGD:S000005967"
/translation="MSQDFVTLVSKDDKEYEISRSAAMISPTLKAMIEGPFRESKGRI
ELKQFDSHILEKAVEYLNYNLKYSGVSEDDDEIPEFEIPTEMSLELLLAADYLSI"
gene <467260..>469656
/gene="VPS16"
/locus_tag="YPL045W"
/gene_synonym="CVT15; SVL6; VAM9; VPT16"
/db_xref="GeneID:856062"
mRNA <467260..>469656
/gene="VPS16"
/locus_tag="YPL045W"
/gene_synonym="CVT15; SVL6; VAM9; VPT16"
/product="tethering complex subunit VPS16"
/transcript_id="NM_001183859.1"
/db_xref="GeneID:856062"
CDS 467260..469656
/gene="VPS16"
/locus_tag="YPL045W"
/gene_synonym="CVT15; SVL6; VAM9; VPT16"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:10944212|PMID:9362071]"
/experiment="EXISTENCE:direct assay:GO:0033263 CORVET
complex [PMID:17488625]"
/experiment="EXISTENCE:direct assay:GO:0035091
phosphatidylinositol binding [PMID:16601699]"
/experiment="EXISTENCE:direct assay:GO:0035542 regulation
of SNARE complex assembly [PMID:18385512]"
/experiment="EXISTENCE:direct assay:GO:0042144 vacuole
fusion, non-autophagic [PMID:10944212]"
/experiment="EXISTENCE:direct assay:GO:0099022 vesicle
tethering [PMID:27307091]"
/experiment="EXISTENCE:mutant phenotype:GO:0006623 protein
targeting to vacuole [PMID:9362071]"
/experiment="EXISTENCE:mutant phenotype:GO:0006895 Golgi
to endosome transport [PMID:11422941]"
/experiment="EXISTENCE:mutant phenotype:GO:0007033 vacuole
organization [PMID:9362071]"
/experiment="EXISTENCE:mutant phenotype:GO:0042144 vacuole
fusion, non-autophagic [PMID:10944212]"
/experiment="EXISTENCE:mutant phenotype:GO:0045324 late
endosome to vacuole transport [PMID:11422941]"
/experiment="EXISTENCE:physical interaction:GO:0030897
HOPS complex [PMID:10944212]"
/note="Subunit of the HOPS and the CORVET complexes; part
of the Class C Vps complex essential for membrane docking
and fusion at Golgi-to-endosome and endosome-to-vacuole
protein transport stages"
/codon_start=1
/product="tethering complex subunit VPS16"
/protein_id="NP_015280.1"
/db_xref="GeneID:856062"
/db_xref="SGD:S000005966"
/translation="MKNPSFDWERLKDVFYRSRAIGELKWPTQYEEFKCALSLTVIAV
EIQDFIQVYNYFGQLLGKINLQRIHEDIIKFEFDKDEKLILVTKSSIKIVKGWSPLTI
ESVPLQDPTIDTIWDYHNGIMLLAKSRDIYKLNGNEWELLYENKDKKYNLLTKNHWSC
NDDSIILLDVDHVYQVSTSNGALLKLITDSSWHKVTISSRGFICLYNMKDNKLQIFRD
PARILMEHNLDSTPDDICWCGNDTVACSFEDEIKLYGPDGLYVTFWYPFTVTNLRAEV
DGLKVITTEKIYFLSRVQPQTSNIFRIGSTEPGAMLVDSFSLLEDHAPKAIEILKNFV
LEKGVLDCIAAAIDEFEPKLQKMLLNAASYGKASLQYKSFDASIFVNACNTIKLLNCF
RSFGIFLTVEEYRCISLKGVIDRLLKYHRYYECIQICKLANERFLLGYVFTEWAKDKI
KGSPDMEDDELLDKIKSRLSVIDMTDTLQMVAVAKVAYLEGRFQLSRNLALLEKNEEA
RIEQLYNLDDDSIALKECIKVQNYSLTISLLIALSKKLTNSQLTKLLIIDMFNNPLYL
YYMRMDKAYLYDFYRQTDRFIDLAHVLLQQGKEQQSLHSFLPQIKDLYSQVQNSEVVN
NTIEQLQRQEKLWIYQESLGKRFAISFTNMTLDQTLSKLIETGQDKQVKEIVKKFKIS
EKKLYHLKCKTLVEAKKFDELLQFAQSRKSPIGYMPFYTYLKSRGHMDKASPYVNMIP
GLSYQEKKKLYVECRGFRDAIQLAGKEKDIPGLKEIYNIIPPNEPELKALANETMSRI
"
gene <469939..>471996
/gene="NOP4"
/locus_tag="YPL043W"
/gene_synonym="NOP77"
/db_xref="GeneID:856063"
mRNA <469939..>471996
/gene="NOP4"
/locus_tag="YPL043W"
/gene_synonym="NOP77"
/product="mRNA-binding ribosome biosynthesis protein NOP4"
/transcript_id="NM_001183857.1"
/db_xref="GeneID:856063"
CDS 469939..471996
/gene="NOP4"
/locus_tag="YPL043W"
/gene_synonym="NOP77"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0000463
maturation of LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:8039505]"
/experiment="EXISTENCE:mutant phenotype:GO:0042273
ribosomal large subunit biogenesis [PMID:8039505]"
/note="Nucleolar protein; essential for processing and
maturation of 27S pre-rRNA and large ribosomal subunit
biogenesis; constituent of 66S pre-ribosomal particles;
contains four RNA recognition motifs (RRMs); human
ortholog RBM28 implicated in ANE syndrome"
/codon_start=1
/product="mRNA-binding ribosome biosynthesis protein NOP4"
/protein_id="NP_015282.1"
/db_xref="GeneID:856063"
/db_xref="SGD:S000005964"
/translation="MEETIENVEVPSSNVSKQNDDGLDMKTLFVRSIPQDVTDEQLAD
FFSNFAPIKHAVVVKDTNKRSRGFGFVSFAVEDDTKEALAKARKTKFNGHILRVDIAK
RRDRSKKTSEVVEKSTPESSEKITGQNNEDEDDADGEDSMLKGKPKLIIRNMPWSCRD
PVKLKKIFGRYGTVVEATIPRKRDGKLCGFAFVTMKKISNCRIALENTKDLKIDGRKV
AVDFAVQKNRWEDYKKAQPEMNDKDDNESGNEDAEENHDDEEDENEEEDRQVDQASKN
KESKRKAQNKREDFSVFVRNVPYDATEESLAPHFSKFGSVKYALPVIDKSTGLAKGTA
FVAFKDQYTYNECIKNAPAAGSTSLLIGDDVMPEYVYEGRVLSITPTLVREDAGRMAE
KNAAKRKEALGKAPGEKDRRNLYLLNEGRVVEGSKMADLLTNTDMEIREKSYKLRVEQ
LKKNPSLHLSMTRLAIRNLPRAMNDKALKALARKAVVEFATEVKNKERHPLSKEEIIR
STKEKYKFMGPDEIEAQKKKDKKSGVVKQAKVIMEVKGSTAGRSRGYGFVEFRDHKNA
LMGLRWLNCHAVTSDEILEGLNDDEKKQVDNDLGKGRRLCVEFAIENSNVVKRRREQL
KQARTKRTRPDNEDTGDVGESENKKPKKEEATTPTNPDDKKMGDDIKRIIGFKRKRKH
AKK"
gene complement(<473040..>474707)
/gene="SSN3"
/locus_tag="YPL042C"
/gene_synonym="CDK8; GIG2; NUT7; RYE5; SRB10; SSX7; UME5;
URR1"
/db_xref="GeneID:856065"
mRNA complement(<473040..>474707)
/gene="SSN3"
/locus_tag="YPL042C"
/gene_synonym="CDK8; GIG2; NUT7; RYE5; SRB10; SSX7; UME5;
URR1"
/product="cyclin-dependent serine/threonine protein kinase
SSN3"
/transcript_id="NM_001183856.1"
/db_xref="GeneID:856065"
CDS complement(473040..474707)
/gene="SSN3"
/locus_tag="YPL042C"
/gene_synonym="CDK8; GIG2; NUT7; RYE5; SRB10; SSX7; UME5;
URR1"
/EC_number="2.7.11.22"
/EC_number="2.7.11.23"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0004693
cyclin-dependent protein serine/threonine kinase activity
[PMID:11331604]"
/experiment="EXISTENCE:direct assay:GO:0008353 RNA
polymerase II CTD heptapeptide repeat kinase activity
[PMID:9702190]"
/experiment="EXISTENCE:direct assay:GO:0016592 mediator
complex [PMID:12200444|PMID:11383511]"
/experiment="EXISTENCE:genetic interaction:GO:0060258
negative regulation of filamentous growth [PMID:12520306]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:16109375]"
/experiment="EXISTENCE:mutant phenotype:GO:0008353 RNA
polymerase II CTD heptapeptide repeat kinase activity
[PMID:9702190]"
/experiment="EXISTENCE:mutant phenotype:GO:0031648 protein
destabilization [PMID:12520306]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:15601835]"
/experiment="EXISTENCE:mutant phenotype:GO:0060258
negative regulation of filamentous growth [PMID:12520306]"
/experiment="EXISTENCE:mutant phenotype:GO:0070481
nuclear-transcribed mRNA catabolic process, non-stop decay
[PMID:17660569]"
/experiment="EXISTENCE:physical interaction:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:11226276]"
/note="Component of the RNA polymerase II mediator
complex; cyclin-dependent protein kinase involved in
phosphorylation of the mediator component Pgd1p and
suppression of transcription activation by RNA polymerase
II; involved in glucose repression"
/codon_start=1
/product="cyclin-dependent serine/threonine protein kinase
SSN3"
/protein_id="NP_015283.1"
/db_xref="GeneID:856065"
/db_xref="SGD:S000005963"
/translation="MYNGKDRAQNSYQPMYQRPMQVQGQQQAQSFVGKKNTIGSVHGK
APMLMANNDVFTIGPYRARKDRMRVSVLEKYEVIGYIAAGTYGKVYKAKRQINSGTNS
ANGSSLNGTNAKIPQFDSTQPKSSSSMDMQANTNALRRNLLKDEGVTPGRIRTTREDV
SPHYNSQKQTLIKKPLTVFYAIKKFKTEKDGVEQLHYTGISQSACREMALCRELHNKH
LTTLVEIFLERKCVHMVYEYAEHDLLQIIHFHSHPEKRMIPPRMVRSIMWQLLDGVSY
LHQNWVLHRDLKPANIMVTIDGCVKIGDLGLARKFHNMLQTLYTGDKVVVTIWYRAPE
LLLGARHYTPAVDLWSVGCIFAELIGLQPIFKGEEAKLDSKKTVPFQVNQLQRILEVL
GTPDQKIWPYLEKYPEYDQITKFPKYRDNLATWYHSAGGRDKHALSLLYHLLNYDPIK
RIDAFNALEHKYFTESDIPVSENVFEGLTYKYPARRIHTNDNDIMNLGSRTKNNTQAS
GITAGAAANALGGLGVNRRILAAAAAAAAAVSGNNASDEPSRKKNRR"
gene complement(<475118..>475741)
/gene="MRX11"
/locus_tag="YPL041C"
/db_xref="GeneID:856066"
mRNA complement(<475118..>475741)
/gene="MRX11"
/locus_tag="YPL041C"
/product="Mrx11p"
/transcript_id="NM_001183855.1"
/db_xref="GeneID:856066"
CDS complement(475118..475741)
/gene="MRX11"
/locus_tag="YPL041C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:26928762]"
/note="Protein that associates with mitochondrial
ribosome; SWAT-GFP and mCherry fusion proteins localize to
the mitochondria; involved in maintenance of telomere
length"
/codon_start=1
/product="Mrx11p"
/protein_id="NP_015284.1"
/db_xref="GeneID:856066"
/db_xref="SGD:S000005962"
/translation="MTVMNLFFRPCQLQMGSGPLELMLKRPTQLTTFMNTRPGGSTQI
RFISGNLDPVKRREDRLRKIFSKSRLLTRLNKNPKFSHYFDRLSEAGTVPTLTSFFIL
HEVTAILPLFLLWWLLYNLDLSDDFKLPNFLNGLMDSCHTAMEKFVGKRYQECLNKNK
LILSGTVAYVTVKLLYPVRIFISIWGAPYFGKWLLLPFQKLKHLIKK"
gene complement(<475993..>479001)
/gene="ISM1"
/locus_tag="YPL040C"
/db_xref="GeneID:856067"
mRNA complement(<475993..>479001)
/gene="ISM1"
/locus_tag="YPL040C"
/product="isoleucine--tRNA ligase ISM1"
/transcript_id="NM_001183854.1"
/db_xref="GeneID:856067"
CDS complement(475993..479001)
/gene="ISM1"
/locus_tag="YPL040C"
/EC_number="6.1.1.5"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278|PMID:24769239]"
/experiment="EXISTENCE:mutant phenotype:GO:0005739
mitochondrion [PMID:7607232]"
/experiment="EXISTENCE:mutant phenotype:GO:0032543
mitochondrial translation [PMID:7607232]"
/note="Mitochondrial isoleucyl-tRNA synthetase; null
mutant is deficient in respiratory growth; human homolog
IARS2 implicated in mitochondrial diseases, can partially
complement yeast null mutant"
/codon_start=1
/product="isoleucine--tRNA ligase ISM1"
/protein_id="NP_015285.1"
/db_xref="GeneID:856067"
/db_xref="SGD:S000005961"
/translation="MKRSRLVPQHIFSIISKRYLAKHAYQKTLNLPKTKFPNRSNLEI
TLRELIPKSSQLVYKEQLRDFFEEFSKLNTTDEKLEFIKEKLFILHDGPPYANGELHL
GHALNKILKDIINRYQLSQGKYIFYKPGWDCHGLPIEIKALKDLSAQQIESISPLKIR
SMALKHAQKAIKRQRETFQHFAILTDWETPYLTMDKDYEINQLNIFKEMYERGLIKRQ
NKPVYWGTETRTALAEGELEYNENHKSIAAYVKFPLEKKSQMDLCKKLGITNNLPIYC
LIWTSTPWTLLSNRAICFNQDFSYSLLRLNSELILVETGSIDKLGLTTNSFETIKQFQ
GTHLNGLYYQNLLVDDKVGRPLLHGAHVTSGTGTGLVHTAPGHGQDDYLIGIQNGLEI
YSPVDHQGRYQLNELPQSVRSIVRDEGDLTKGRQVLDAETAKIILCKLSDLNLLYKSH
EYTHSYPYDWRSKKPVIIRATPQWFADLHDVKNLALESISRVKFCPKRGYSRLSSFMK
SRNEWCISRQRSWGIPILSFYKKSEPDSVLMNSEILAHAIEKIKQKGINAWFNDKDND
MKEWLPEKYHDVAHEYCRSQDTMDVWFDSGSSWSVIKDFYEKSLKLSKLPSPLYQVCL
EGSDQHRGWFQSSLLTKVASSNVPVAPYEEVITHGFTLDENGLKMSKSVGNTISPEAI
IRGDENLGLPALGVDGLRYLIAQSNFTTDIVAGPTVMKHVGEALKKVRLTFRYLLSNL
QKSQDFNLLPIEQLRRVDQYTLYKINELLETTREHYQKYNFSKVLITLQYHLNNELSA
FYFDISKDILYSNQISSLARRQVQTTLVHILNAYRAILAPILPVMVQEVWKYIPEGWL
QGQEHIDINPMRGKWPFLDSNTEIVTSFENFELKILKQFQEEFKRLSLEEGVTKTTHS
HVTIFTKHHLPFSSDELCDILQSSAVDILQMDDNNNSLPTIELGSGINVQILVERSKR
HNCPRCWKANSAEEDKLCDRCKEAVDHLMS"
gene <479225..>480175
/locus_tag="YPL039W"
/db_xref="GeneID:856068"
mRNA <479225..>480175
/locus_tag="YPL039W"
/product="uncharacterized protein"
/transcript_id="NM_001183853.1"
/db_xref="GeneID:856068"
CDS 479225..480175
/locus_tag="YPL039W"
/note="hypothetical protein; YPL039W is not an essential
gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015286.1"
/db_xref="GeneID:856068"
/db_xref="SGD:S000005960"
/translation="MLKEEYNLSGADNMASAELSFKKQKVVLKKFMIAQVTKGIMQRY
ASLLVTMPSDDDNAIIGTNHLKTTKFLEIILHRAKSSHLQFKKVCCIVIKFLDCCSKE
TNYMKFLKFSLHKLFVAAFILSVPNVVGDDRDRITTRDETYHLYSQITGLPLEEVINC
CSIVRPVLIRRSRQQRRQMLSRREQHSYFLRSSFMNSNSSASPFFSTNRSADDLHVHT
RAYSLHSHSDGEGQDRRSEHEETHSMGADADTYRQTTFIPDTPNGLHSRSLIECGIEP
TQVSDSGEWSGQSNGYVLVTELQEFNKMGKKLVQEAFRIV"
gene <480182..>480373
/locus_tag="YPL038W-A"
/db_xref="GeneID:1466521"
mRNA <480182..>480373
/locus_tag="YPL038W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184580.1"
/db_xref="GeneID:1466521"
CDS 480182..480373
/locus_tag="YPL038W-A"
/note="hypothetical protein; identified by fungal homology
and RT-PCR"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878182.1"
/db_xref="GeneID:1466521"
/db_xref="SGD:S000028588"
/translation="MVCRFVHHSRVIFISIYDFLSTKGKKNMYNYTQEKKTKQKNTFT
QASIYYENFFESYRTISCL"
gene <480535..>481068
/gene="MET31"
/locus_tag="YPL038W"
/db_xref="GeneID:856069"
mRNA <480535..>481068
/gene="MET31"
/locus_tag="YPL038W"
/product="Met31p"
/transcript_id="NM_001183852.1"
/db_xref="GeneID:856069"
CDS 480535..481068
/gene="MET31"
/locus_tag="YPL038W"
/experiment="EXISTENCE:direct assay:GO:0000981 DNA-binding
transcription factor activity, RNA polymerase II-specific
[PMID:9799240]"
/experiment="EXISTENCE:direct assay:GO:0000987
cis-regulatory region sequence-specific DNA binding
[PMID:9199298]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0006357 regulation
of transcription by RNA polymerase II [PMID:9199298]"
/experiment="EXISTENCE:direct assay:GO:2000282 regulation
of amino acid biosynthetic process [PMID:9199298]"
/experiment="EXISTENCE:genetic interaction:GO:0000981
DNA-binding transcription factor activity, RNA polymerase
II-specific [PMID:9799240]"
/experiment="EXISTENCE:genetic interaction:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:9199298]"
/experiment="EXISTENCE:genetic interaction:GO:2000282
regulation of amino acid biosynthetic process
[PMID:9199298]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:22696683]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:9199298]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:22696683]"
/experiment="EXISTENCE:mutant phenotype:GO:2000282
regulation of amino acid biosynthetic process
[PMID:9199298]"
/experiment="EXISTENCE:physical interaction:GO:0000981
DNA-binding transcription factor activity, RNA polymerase
II-specific [PMID:9799240]"
/note="Zinc-finger DNA-binding transcription factor;
targets strong transcriptional activator Met4p to
promoters of sulfur metabolic genes; involved in
transcriptional regulation of the methionine biosynthetic
genes; feedforward loop controlling expression of MET32
and the lack of such a loop for MET31 may account for the
differential actions of Met31p and Met32p; MET31 has a
paralog, MET32, that arose from the whole genome
duplication"
/codon_start=1
/product="Met31p"
/protein_id="NP_015287.1"
/db_xref="GeneID:856069"
/db_xref="SGD:S000005959"
/translation="MKLAQDMNVDEIFLKQAAEAIAVISSSPTHTDPIIRELLHRIRQ
SSPLSAVIPAPENVLKAGEPENMARGLIRIPETQTKRTGGNNHSKEGAQLYSCAKCQL
KFSRSSDLRRHEKVHSLVLPHICSNCGKGFARKDALKRHSNTLTCQRNRKKLSEGSDV
DVDELIKDAIKNGTGLL"
gene complement(<481428..>481901)
/gene="EGD1"
/locus_tag="YPL037C"
/db_xref="GeneID:856070"
mRNA complement(<481428..>481901)
/gene="EGD1"
/locus_tag="YPL037C"
/product="Egd1p"
/transcript_id="NM_001183851.1"
/db_xref="GeneID:856070"
CDS complement(481428..481901)
/gene="EGD1"
/locus_tag="YPL037C"
/experiment="EXISTENCE:direct assay:GO:0005854 nascent
polypeptide-associated complex [PMID:10219998]"
/experiment="EXISTENCE:genetic interaction:GO:0006613
cotranslational protein targeting to membrane
[PMID:10518932]"
/experiment="EXISTENCE:mutant phenotype:GO:0000423
mitophagy [PMID:38177147]"
/experiment="EXISTENCE:mutant phenotype:GO:0016236
macroautophagy [PMID:19793921]"
/experiment="EXISTENCE:mutant phenotype:GO:0051082
unfolded protein binding [PMID:9482879]"
/note="Subunit beta1 of the nascent polypeptide-associated
complex (NAC); involved in protein targeting to
mitochondria; peripheral component of cytoplasmic
ribosomes; regulates Atg32p phosphorylation to promote
respiration-induced mitophagy; enhances DNA binding of the
Gal4p; homolog of human BTF3b; EGD1 has a paralog, BTT1,
that arose from the whole genome duplication"
/codon_start=1
/product="Egd1p"
/protein_id="NP_015288.1"
/db_xref="GeneID:856070"
/db_xref="SGD:S000005958"
/translation="MPIDQEKLAKLQKLSANNKVGGTRRKLNKKAGSSAGANKDDTKL
QSQLAKLHAVTIDNVAEANFFKDDGKVMHFNKVGVQVAAQHNTSVFYGLPQEKNLQDL
FPGIISQLGPEAIQALSQLAAQMEKHEAKAPADAEKKDEAIPELVEGQTFDADVE"
gene <482843..>485686
/gene="PMA2"
/locus_tag="YPL036W"
/db_xref="GeneID:856071"
mRNA <482843..>485686
/gene="PMA2"
/locus_tag="YPL036W"
/product="H(+)-exporting P2-type ATPase PMA2"
/transcript_id="NM_001183850.1"
/db_xref="GeneID:856071"
CDS 482843..485686
/gene="PMA2"
/locus_tag="YPL036W"
/EC_number="7.1.2.1"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:8396147|PMID:16622836]"
/experiment="EXISTENCE:direct assay:GO:0008553 P-type
proton-exporting transporter activity [PMID:8396147]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:1902600 proton
transmembrane transport [PMID:8396147]"
/note="Plasma membrane H+-ATPase; isoform of Pma1p,
involved in pumping protons out of the cell; regulator of
cytoplasmic pH and plasma membrane potential"
/codon_start=1
/product="H(+)-exporting P2-type ATPase PMA2"
/protein_id="NP_015289.1"
/db_xref="GeneID:856071"
/db_xref="SGD:S000005957"
/translation="MSSTEAKQYKEKPSKEYLHASDGDDPANNSAASSSSSSSTSTSA
SSSAAAVPRKAAAASAADDSDSDEDIDQLIDELQSNYGEGDESGEEEVRTDGVHAGQR
VVPEKDLSTDPAYGLTSDEVARRRKKYGLNQMAEENESLIVKFLMFFVGPIQFVMEAA
AILAAGLSDWVDVGVICALLLLNASVGFIQEFQAGSIVDELKKTLANTATVIRDGQLI
EIPANEVVPGEILQLESGTIAPADGRIVTEDCFLQIDQSAITGESLAAEKHYGDEVFS
SSTVKTGEAFMVVTATGDNTFVGRAAALVGQASGVEGHFTEVLNGIGIILLVLVIATL
LLVWTACFYRTVGIVSILRYTLGITIIGVPVGLPAVVTTTMAVGAAYLAKKQAIVQKL
SAIESLAGVEILCSDKTGTLTKNKLSLHEPYTVEGVSPDDLMLTACLAASRKKKGLDA
IDKAFLKSLIEYPKAKDALTKYKVLEFHPFDPVSKKVTAVVESPEGERIVCVKGAPLF
VLKTVEEDHPIPEDVHENYENKVAELASRGFRALGVARKRGEGHWEILGVMPCMDPPR
DDTAQTINEARNLGLRIKMLTGDAVGIAKETCRQLGLGTNIYNAERLGLGGGGDMPGS
ELADFVENADGFAEVFPQHKYRVVEILQNRGYLVAMTGDGVNDAPSLKKADTGIAVEG
ATDAARSAADIVFLAPGLSAIIDALKTSRQIFHRMYSYVVYRIALSLHLEIFLGLWIA
ILNNSLDINLIVFIAIFADVATLTIAYDNAPYAPEPVKWNLPRLWGMSIILGIVLAIG
SWITLTTMFLPNGGIIQNFGAMNGVMFLQISLTENWLIFVTRAAGPFWSSIPSWQLAG
AVFAVDIIATMFTLFGWWSENWTDIVSVVRVWIWSIGIFCVLGGFYYIMSTSQAFDRL
MNGKSLKEKKSTRSVEDFMAAMQRVSTQHEKSS"
gene <486712..>487209
/gene="YFS1"
/locus_tag="YPL034W"
/db_xref="GeneID:856072"
mRNA <486712..>487209
/gene="YFS1"
/locus_tag="YPL034W"
/product="uncharacterized protein"
/transcript_id="NM_001183848.1"
/db_xref="GeneID:856072"
CDS 486712..487209
/gene="YFS1"
/locus_tag="YPL034W"
/note="hypothetical protein; mRNA translated through
programmed +1 translational frameshifting; not an
essential gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015291.1"
/db_xref="GeneID:856072"
/db_xref="SGD:S000005955"
/translation="MTTRKTVDSRLLEWQTTCKHPVINLTPEKVDKLYHLKLKSESKN
ISSNRLLPISLSSLQKKMEKLFIKDKSHSHKPSLPDPKVPTLRTYKDGGFFISGKGSM
KLPDIESAIHKFLWKKYGKGLVYCYGCDPTGKKRHTEWFNVPVLELPSVLRLIDSYCL
GGESR"
gene complement(<487362..>488207)
/gene="SRL4"
/locus_tag="YPL033C"
/db_xref="GeneID:856074"
mRNA complement(<487362..>488207)
/gene="SRL4"
/locus_tag="YPL033C"
/product="Srl4p"
/transcript_id="NM_001183847.1"
/db_xref="GeneID:856074"
CDS complement(487362..488207)
/gene="SRL4"
/locus_tag="YPL033C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24390141]"
/experiment="EXISTENCE:mutant phenotype:GO:0009200
deoxyribonucleoside triphosphate metabolic process
[PMID:18337697]"
/note="hypothetical protein; involved in regulation of
dNTP production; null mutant suppresses the lethality of
lcd1 and rad53 mutations; expression is induced by Kar4p"
/codon_start=1
/product="Srl4p"
/protein_id="NP_015292.1"
/db_xref="GeneID:856074"
/db_xref="SGD:S000005954"
/translation="MKKTIYKVLVSFYQYVGLGKKFHPSHDTVLIIGGSSNELGIELC
ETFIEDYHTKVINIDTIDSINGKNARRSEKLYTFISCKDFSDIKCLEESMLYLQNLEI
IPTVLINNMQEGIESTLLKEDKFLRLDEESLNEFEKIVRYNLQSVILITKFCLSNIFP
KVQAEAQEKAKGFYIVNISSVLTLKPCKSGTHFITSKCGINSFHDGITSELKLKDSNL
NVKTLIAYLPSFESEAHWKRLSPSISKHLVHCLLEGRYGDTILESKRSIGDILLITGF
KSSFT"
gene complement(<488887..>491364)
/gene="SVL3"
/locus_tag="YPL032C"
/db_xref="GeneID:856075"
mRNA complement(<488887..>491364)
/gene="SVL3"
/locus_tag="YPL032C"
/product="Svl3p"
/transcript_id="NM_001183846.1"
/db_xref="GeneID:856075"
CDS complement(488887..491364)
/gene="SVL3"
/locus_tag="YPL032C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005933 cellular
bud [PMID:26928762|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:32491201|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:32491201|PMID:22842922|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0006897
endocytosis [PMID:9751732]"
/note="hypothetical protein; mutant phenotype suggests a
potential role in vacuolar function; green fluorescent
protein (GFP)-fusion protein localizes to the cell
periphery, cytoplasm, bud, and bud neck; relocalizes from
bud neck to cytoplasm upon DNA replication stress; SVL3
has a paralog, PAM1, that arose from the whole genome
duplication"
/codon_start=1
/product="Svl3p"
/protein_id="NP_015293.1"
/db_xref="GeneID:856075"
/db_xref="SGD:S000005953"
/translation="MSSSSLRVLAIGNNPNILFYTSRFQLAKNIDLYHVNDSKSCQFE
IETEYYGKDRFELENHFTSIEHLTEALSSKSSEAVFDIIIMSAPSLQELSSLASKLTS
IIDSNTKIFLESSGFIQLEPFVKLSMESPHVNVFSILTDLDIRQIGPNHFKHFPSTAK
ENTIYLGESKSSTEKYSSGVITLLTTFEKLFAKLFSNIKINLCNFSSIEFLSQQWKLA
ISRICFDPLLIMFEQENPSDLDQQIIAKPLISGLVTEIITVAKTMGARLNSSHDNENS
LLSLWKNSYHSTNKPPALVYHFIHQTTPLNIDILLLQTILLADDFGIKTPYLEFLYSV
LSQFERLNSGKSKWFIRSDEKTQILQSLQKSQKNESALQTQITSLQGQISKLRQELLM
QAKQHEMETNELKEKHQVALKAQAQAQAQAQSQAQTSIEALTPTEATNQSDTNEYKAT
GTPNLRDIEDMALYSVNYGDSPVRSPPPVVSSQPQMNSPLSSHSQTFGENNGTNDKLL
QERELQLRKKELELQERELEFQKRALQQQRFNNSNNSIPRKPSFPQLQQSANVRSNSR
GMHGTNGAMSQPASAGNFVDPISSSIAAYDPQQPPSLPLQQPQQSVQVQPFHSHSIKP
TSRKNRNSNMPNIGNPSSINMSDFGRPPNNSSQTRLNSMPTHSIVNQNRLRSQQSKNK
LNMPHATNPNNTFNQVPAPSLNNHVPTQRQFSSSTMIEVTNNNNKVNNSSSNPDISTN
SVVHNAMQFTNTNNNTSSTVDINDPKNIAPPPTTSVSAPSTPTLSSSSQMANMASPST
DNGDNEEKNGGKKKRFGLFKKKNKSKK"
gene complement(<492018..>493037)
/gene="PHO85"
/locus_tag="YPL031C"
/gene_synonym="LDB15"
/db_xref="GeneID:856076"
mRNA complement(join(<492018..492918,493021..>493037))
/gene="PHO85"
/locus_tag="YPL031C"
/gene_synonym="LDB15"
/product="cyclin-dependent serine/threonine-protein kinase
PHO85"
/transcript_id="NM_001183845.1"
/db_xref="GeneID:856076"
CDS complement(join(492018..492918,493021..493037))
/gene="PHO85"
/locus_tag="YPL031C"
/gene_synonym="LDB15"
/EC_number="2.7.11.22"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0004693
cyclin-dependent protein serine/threonine kinase activity
[PMID:7973730|PMID:38233717|PMID:9584169|PMID:12407105|PMI
D:7973731|PMID:19823668|PMID:8108735]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9853758]"
/experiment="EXISTENCE:direct assay:GO:0019216 regulation
of lipid metabolic process [PMID:31678512]"
/experiment="EXISTENCE:direct assay:GO:0032880 regulation
of protein localization [PMID:8539622]"
/experiment="EXISTENCE:direct assay:GO:0046822 regulation
of nucleocytoplasmic transport [PMID:31422915]"
/experiment="EXISTENCE:direct assay:GO:0055088 lipid
homeostasis [PMID:31678512]"
/experiment="EXISTENCE:direct assay:GO:0071073 positive
regulation of phospholipid biosynthetic process
[PMID:27743455]"
/experiment="EXISTENCE:direct assay:GO:1901987 regulation
of cell cycle phase transition [PMID:29146254]"
/experiment="EXISTENCE:direct assay:GO:1990860 Pho85-Pho80
CDK-cyclin complex [PMID:8108735]"
/experiment="EXISTENCE:genetic interaction:GO:0000082 G1/S
transition of mitotic cell cycle
[PMID:15738404|PMID:19823668]"
/experiment="EXISTENCE:genetic interaction:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:3915785]"
/experiment="EXISTENCE:genetic interaction:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:19823668|PMID:15738404]"
/experiment="EXISTENCE:genetic interaction:GO:0006974 DNA
damage response [PMID:16964260]"
/experiment="EXISTENCE:genetic interaction:GO:0031505
fungal-type cell wall organization [PMID:12077337]"
/experiment="EXISTENCE:genetic interaction:GO:0031647
regulation of protein stability [PMID:16611745]"
/experiment="EXISTENCE:genetic interaction:GO:0032878
regulation of establishment or maintenance of cell
polarity [PMID:17853895]"
/experiment="EXISTENCE:genetic interaction:GO:0045936
negative regulation of phosphate metabolic process
[PMID:3915785]"
/experiment="EXISTENCE:genetic interaction:GO:0050849
negative regulation of calcium-mediated signaling
[PMID:16455487]"
/experiment="EXISTENCE:mutant phenotype:GO:0000082 G1/S
transition of mitotic cell cycle [PMID:19823668]"
/experiment="EXISTENCE:mutant phenotype:GO:0004693
cyclin-dependent protein serine/threonine kinase activity
[PMID:38233717|PMID:9593297]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:19823668]"
/experiment="EXISTENCE:mutant phenotype:GO:0006974 DNA
damage response [PMID:16964260]"
/experiment="EXISTENCE:mutant phenotype:GO:0016239
positive regulation of macroautophagy [PMID:20417603]"
/experiment="EXISTENCE:mutant phenotype:GO:0016242
negative regulation of macroautophagy [PMID:20417603]"
/experiment="EXISTENCE:mutant phenotype:GO:0031647
regulation of protein stability [PMID:16508019]"
/experiment="EXISTENCE:mutant phenotype:GO:0031648 protein
destabilization [PMID:38233717]"
/experiment="EXISTENCE:mutant phenotype:GO:0045719
negative regulation of glycogen biosynthetic process
[PMID:9584169]"
/experiment="EXISTENCE:physical interaction:GO:0000307
cyclin-dependent protein kinase holoenzyme complex
[PMID:7973730|PMID:7973731|PMID:16611745|PMID:8108735|PMID
:9584169]"
/note="Cyclin-dependent kinase; has ten cyclin partners;
involved in regulating the cellular response to nutrient
levels, environmental conditions and progression through
the cell cycle; involved in the hyperphosphorylation of
Srl3p (Whi7p), regulating protein stability, mRNA levels
and promotion of START; human lissencephaly-associated
homolog CDK5 functionally complements null mutation"
/codon_start=1
/product="cyclin-dependent serine/threonine-protein kinase
PHO85"
/protein_id="NP_015294.1"
/db_xref="GeneID:856076"
/db_xref="SGD:S000005952"
/translation="MSSSSQFKQLEKLGNGTYATVYKGLNKTTGVYVALKEVKLDSEE
GTPSTAIREISLMKELKHENIVRLYDVIHTENKLTLVFEFMDNDLKKYMDSRTVGNTP
RGLELNLVKYFQWQLLQGLAFCHENKILHRDLKPQNLLINKRGQLKLGDFGLARAFGI
PVNTFSSEVVTLWYRAPDVLMGSRTYSTSIDIWSCGCILAEMITGKPLFPGTNDEEQL
KLIFDIMGTPNESLWPSVTKLPKYNPNIQQRPPRDLRQVLQPHTKEPLDGNLMDFLHG
LLQLNPDMRLSAKQALHHPWFAEYYHHAS"
gene <493543..>495246
/gene="TRM44"
/locus_tag="YPL030W"
/db_xref="GeneID:856077"
mRNA <493543..>495246
/gene="TRM44"
/locus_tag="YPL030W"
/product="tRNA (uracil) methyltransferase"
/transcript_id="NM_001183844.1"
/db_xref="GeneID:856077"
CDS 493543..495246
/gene="TRM44"
/locus_tag="YPL030W"
/EC_number="2.1.1.211"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0016300 tRNA
(uridine) methyltransferase activity [PMID:18025252]"
/experiment="EXISTENCE:direct assay:GO:0030488 tRNA
methylation [PMID:18025252]"
/experiment="EXISTENCE:mutant phenotype:GO:0002128 tRNA
nucleoside ribose methylation [PMID:32053677]"
/experiment="EXISTENCE:mutant phenotype:GO:0016300 tRNA
(uridine) methyltransferase activity [PMID:18025252]"
/experiment="EXISTENCE:mutant phenotype:GO:0030488 tRNA
methylation [PMID:18025252]"
/note="tRNA(Ser) Um(44) 2'-O-methyltransferase; involved
in maintaining levels of the tRNA-Ser species tS(CGA) and
tS(UGA); conserved among metazoans and fungi but there
does not appear to be a homolog in plants; TRM44 is a
non-essential gene"
/codon_start=1
/product="tRNA (uracil) methyltransferase"
/protein_id="NP_015295.1"
/db_xref="GeneID:856077"
/db_xref="SGD:S000005951"
/translation="MTGDGSAHISKNNQNQHKDRFKFIVNDKSILGPQWLSLYQTDGK
VTFAKSHFEQAMMNVIREPNINSTVILRADILKEINHAAEAGSEPKFDESVLKKFEID
NGNESGEEDVKKINIEDLNIRSCETSESLKLSPVHEFVRRIIPRNFYKDAIINQTCLI
LNSKDPNFQETSLIVYTPHINSEKDCPFYIPRTQSVGILLHQSVLSVHYIPFPEDKTA
FTDESERVVRTAYRLLQTANKHSKGVMQGYEKRVNHDQVVNKVNFQNTYIVLKKKYSK
FLVENWAESTDPKKHVFEDIAIAAFLIELWIKVYGPDFRSKMQFRDLGCGNGALCYIL
LSESIKGLGIDARKRKSWSIYPPEVQSSLKEQVIIPSILLRPHPALKRQVPHLEHNGR
FFPVKVTHEVIAPATVVYSSEDLLKSPQVNTAEFPPDTFIIGNHSDELTCWIPLLGHP
YMVIPCCSHNFSGQRVRFNVRKRSPRSNEIKNQNNSKSTYSGLVDHVEYISSRVGWKV
EKEMLRIPSTRNAAIIGVENATLKHFPTQAVYDMIWEDGGAEGWIQNTMSLLKRNPRN
H"
gene <495506..>497719
/gene="SUV3"
/locus_tag="YPL029W"
/gene_synonym="LPB2"
/db_xref="GeneID:856078"
mRNA <495506..>497719
/gene="SUV3"
/locus_tag="YPL029W"
/gene_synonym="LPB2"
/product="ATP-dependent RNA helicase SUV3"
/transcript_id="NM_001183843.1"
/db_xref="GeneID:856078"
CDS 495506..497719
/gene="SUV3"
/locus_tag="YPL029W"
/gene_synonym="LPB2"
/EC_number="3.6.4.13"
/experiment="EXISTENCE:direct assay:GO:0003724 RNA
helicase activity [PMID:17658549|PMID:12426313]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0008859
exoribonuclease II activity [PMID:17658549]"
/experiment="EXISTENCE:direct assay:GO:0045025
mitochondrial degradosome [PMID:12426313]"
/experiment="EXISTENCE:genetic interaction:GO:0000372
Group I intron splicing [PMID:20064926]"
/experiment="EXISTENCE:mutant phenotype:GO:0000957
mitochondrial RNA catabolic process [PMID:12426313]"
/experiment="EXISTENCE:mutant phenotype:GO:0006264
mitochondrial DNA replication [PMID:21911497]"
/note="ATP-dependent RNA helicase; component of the
mitochondrial degradosome along with the RNase Dss1p; the
degradosome associates with the ribosome and mediates RNA
turnover; also required during splicing of the COX1
AI5_beta intron; expression of a processed form of human
homolog SUPV3L1 carrying an N-terminal deletion of 46
amino acids rescues yeast suv3 null mutant"
/codon_start=1
/product="ATP-dependent RNA helicase SUV3"
/protein_id="NP_015296.1"
/db_xref="GeneID:856078"
/db_xref="SGD:S000005950"
/translation="MALVKYSTVFFPLRSLRLFVSIKKAYYHSEPHSIDLFHDKDWIV
KRPKFLNLPKNEHSKLDIFQFNFNKSESNNVYLQDSSFKDNLDKAMQFIYNDKLSSLD
AKQVPIKNLAWLKLRDYIYQQLKDPKLQAKTYVPSVSEIIHPSSPGNLISLLINCNKI
SNLVWKSVLKYSLSNNITTLDKFIHVLQQTFDHVYEQEILPMMTNTDDTDGAHNVDIT
NPAEWFPEARKIRRHIIMHIGPTNSGKTYRALQKLKSVDRGYYAGPLRLLAREVYDRF
HAEKIRCNLLTGEEVIRDLDDRGNSAGLTSGTVEMVPINQKFDVVVLDEIQMMSDGDR
GWAWTNALLGVVSKEVHLCGEKSVLPLVKSIVKMTGDKLTINEYERLGKLSVEEKPIK
DGIKGLRKGDCVVAFSKKKILDLKLKIEKDTNLKVAVIYGSLPPETRVQQAALFNNGE
YDIMVASDAIGMGLNLSIDRVVFTTNMKYNGEELMEMTSSQIKQIGGRAGRFKSRSAS
GGVPQGFITSFESKVLKSVRKAIEAPVEYLKTAVTWPTDEICAQLMTQFPPGTPTSVL
LQTISDELEKSSDNLFTLSDLKSKLKVIGLFEHMEDIPFFDKLKLSNAPVKDMPMVTK
AFTKFCETIAKRHTRGLLSYRLPFNLLDYNCIPNESYSLEVYESLYNIITLYFWLSNR
YPNYFIDMESAKDLKYFCEMIIFEKLDRLKKNPYAHKPFGSTRGHLSSSRRRLRT"
gene <498096..>499292
/gene="ERG10"
/locus_tag="YPL028W"
/gene_synonym="LPB3; TSM0115"
/db_xref="GeneID:856079"
mRNA <498096..>499292
/gene="ERG10"
/locus_tag="YPL028W"
/gene_synonym="LPB3; TSM0115"
/product="acetyl-CoA C-acetyltransferase"
/transcript_id="NM_001183842.1"
/db_xref="GeneID:856079"
CDS 498096..499292
/gene="ERG10"
/locus_tag="YPL028W"
/gene_synonym="LPB3; TSM0115"
/EC_number="2.3.1.9"
/experiment="EXISTENCE:direct assay:GO:0003985 acetyl-CoA
C-acetyltransferase activity [PMID:5571829|PMID:7989303]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:5571830]"
/experiment="EXISTENCE:direct assay:GO:0006696 ergosterol
biosynthetic process [PMID:5571829]"
/experiment="EXISTENCE:mutant phenotype:GO:0006696
ergosterol biosynthetic process [PMID:200835]"
/note="Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA
thiolase); cytosolic enzyme that transfers an acetyl group
from one acetyl-CoA molecule to another, forming
acetoacetyl-CoA; involved in the first step in mevalonate
biosynthesis; human ACAT1 functionally complements the
growth defect caused by repression of ERG10 expression"
/codon_start=1
/product="acetyl-CoA C-acetyltransferase"
/protein_id="NP_015297.1"
/db_xref="GeneID:856079"
/db_xref="SGD:S000005949"
/translation="MSQNVYIVSTARTPIGSFQGSLSSKTAVELGAVALKGALAKVPE
LDASKDFDEIIFGNVLSANLGQAPARQVALAAGLSNHIVASTVNKVCASAMKAIILGA
QSIKCGNADVVVAGGCESMTNAPYYMPAARAGAKFGQTVLVDGVERDGLNDAYDGLAM
GVHAEKCARDWDITREQQDNFAIESYQKSQKSQKEGKFDNEIVPVTIKGFRGKPDTQV
TKDEEPARLHVEKLRSARTVFQKENGTVTAANASPINDGAAAVILVSEKVLKEKNLKP
LAIIKGWGEAAHQPADFTWAPSLAVPKALKHAGIEDINSVDYFEFNEAFSVVGLVNTK
ILKLDPSKVNVYGGAVALGHPLGCSGARVVVTLLSILQQEGGKIGVAAICNGGGGASS
IVIEKI"
gene <499665..>500402
/gene="SMA1"
/locus_tag="YPL027W"
/db_xref="GeneID:856080"
mRNA <499665..>500402
/gene="SMA1"
/locus_tag="YPL027W"
/product="Sma1p"
/transcript_id="NM_001183841.1"
/db_xref="GeneID:856080"
CDS 499665..500402
/gene="SMA1"
/locus_tag="YPL027W"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:16148007|PMID:24390141]"
/experiment="EXISTENCE:mutant phenotype:GO:0032120
ascospore-type prospore membrane formation
[PMID:11470404|PMID:16148007]"
/note="hypothetical protein involved in prospore membrane
assembly; involved in the assembly of the prospore
membrane during sporulation; interacts with Spo14p"
/codon_start=1
/product="Sma1p"
/protein_id="NP_015298.1"
/db_xref="GeneID:856080"
/db_xref="SGD:S000005948"
/translation="MACTNDGPNKYPEIVSVKHLFQHSGSKHEFSAGKRFSKSIGKIF
KRNSALKTSRTETANHKMELKKREGVTLLPPVPESLLHKLNSWLETFSSTKNMKIEEN
KIVINEKEIRDSVSYYPDKNGGSAVFCYLPDLVLYYKPPIKVTGKQCPIKRSPWESME
IQYQKFMYPLERLERQFEEVPFRPWYFAMRLKELYRCCERSFTNAANRGKARLLRGKQ
RTKKSYHKTVNLVSAKISTHSNAPSPG"
gene complement(<500675..>502183)
/gene="SKS1"
/locus_tag="YPL026C"
/gene_synonym="SHA3"
/db_xref="GeneID:856081"
mRNA complement(<500675..>502183)
/gene="SKS1"
/locus_tag="YPL026C"
/gene_synonym="SHA3"
/product="putative serine/threonine protein kinase SKS1"
/transcript_id="NM_001183840.1"
/db_xref="GeneID:856081"
CDS complement(500675..502183)
/gene="SKS1"
/locus_tag="YPL026C"
/gene_synonym="SHA3"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:18417610]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0010827
regulation of D-glucose transmembrane transport
[PMID:8948096]"
/experiment="EXISTENCE:genetic interaction:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:8948096]"
/experiment="EXISTENCE:mutant phenotype:GO:0007124
pseudohyphal growth [PMID:24603354]"
/note="Putative serine/threonine protein kinase; involved
in the adaptation to low concentrations of glucose
independent of the SNF3 regulated pathway; SKS1 has a
paralog, VHS1, that arose from the whole genome
duplication"
/codon_start=1
/product="putative serine/threonine protein kinase SKS1"
/protein_id="NP_015299.1"
/db_xref="GeneID:856081"
/db_xref="SGD:S000005947"
/translation="MLSDCLLNNFRITAQIGSGAYGLVFHVVDILTSREYAVKTVFKS
SSMDEFYNKNGLNNNSQVARTTLLQTQLYHFFKSFQKKLFLPSVDLDSILQLTENELN
RLPHYREIAFQLRVQSHGNIVKIHQVLESSIATFIVMDYYDRDLFTSIVDDKHFVNHG
ILIKKVFLQLCSALDHCHRLGIYHCDIKPENVLLDRNDNAYLCDFGLSTKSKYLAPNV
CVGSSYYMAPERILYCLNTTTNGIHVDECCSSLPTDTGDIWSLGIILINLTCIRNPWL
KAHQKEDNTFQHFANDNNVLKKILPISDELFTVLTKILQLNPYTRIDMKTLMSEVSSL
TSFTREGPLSQVPILSSEVYMTHIIRNENLFLSDLSHFSADQEQQQQQQQQQQQVQEQ
EQEQKQEQIQNQEQAQQQQEEEDAEPESDIPSTYNSDGSMEKYEYTNNHNNSTFLTSS
MDSTPYQSDIDDVSASKDCKFQQDTLRNRLLCLQMNFSTLTDGPNEKWLPDY"
gene <503517..>504242
/gene="RMI1"
/locus_tag="YPL024W"
/gene_synonym="NCE4"
/db_xref="GeneID:856083"
mRNA <503517..>504242
/gene="RMI1"
/locus_tag="YPL024W"
/gene_synonym="NCE4"
/product="Rmi1p"
/transcript_id="NM_001183838.1"
/db_xref="GeneID:856083"
CDS 503517..504242
/gene="RMI1"
/locus_tag="YPL024W"
/gene_synonym="NCE4"
/experiment="EXISTENCE:direct assay:GO:0000400 four-way
junction DNA binding [PMID:15899853]"
/experiment="EXISTENCE:direct assay:GO:0003697
single-stranded DNA binding [PMID:15899853]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006265 DNA
topological change [PMID:22885009]"
/experiment="EXISTENCE:direct assay:GO:0008047 enzyme
activator activity [PMID:17693398]"
/experiment="EXISTENCE:direct assay:GO:0031422 RecQ family
helicase-topoisomerase III complex [PMID:15899853]"
/experiment="EXISTENCE:direct assay:GO:0043388 positive
regulation of DNA binding [PMID:17693398]"
/experiment="EXISTENCE:direct assay:GO:0043596 nuclear
replication fork [PMID:23036200]"
/experiment="EXISTENCE:genetic interaction:GO:0006974 DNA
damage response [PMID:15889139]"
/experiment="EXISTENCE:physical interaction:GO:0031422
RecQ family helicase-topoisomerase III complex
[PMID:15889139]"
/note="Subunit of the RecQ (Sgs1p) - Topo III (Top3p)
complex; stimulates superhelical relaxing, DNA
catenation/decatenation and ssDNA binding activities of
Top3p; involved in response to DNA damage; functions in S
phase-mediated cohesion establishment via a pathway
involving the Ctf18-RFC complex and Mrc1p; stimulates
Top3p DNA catenation/decatenation activity; null mutants
display increased rates of recombination and delayed S
phase"
/codon_start=1
/product="Rmi1p"
/protein_id="NP_015301.1"
/db_xref="GeneID:856083"
/db_xref="SGD:S000005945"
/translation="MSFSSILSQDITDDITPPAYSATLGSREQIVFRAYQNEPWLAGT
ASNLILDKKLVIVDRELLFQVLMVENITKSKLTQIDDIKTKLDPKKQKVDRLRSGAQG
NGAKKYEVITQVDMEDDGNVADNNCAKENNSNNNSSAAKNKAVFKLTLQSKSGDVFFA
INSTPISWSSCMLGSKIVILPGTVFNRGVFILKDSQVIFLGGINRVWNENRDQKFCDY
LESKLQRDKQLVNGGSKKRKAND"
gene complement(<504339..>506312)
/gene="MET12"
/locus_tag="YPL023C"
/db_xref="GeneID:856084"
mRNA complement(<504339..>506312)
/gene="MET12"
/locus_tag="YPL023C"
/product="methylenetetrahydrofolate reductase (NAD(P)H)
MET12"
/transcript_id="NM_001183837.1"
/db_xref="GeneID:856084"
CDS complement(504339..506312)
/gene="MET12"
/locus_tag="YPL023C"
/EC_number="1.5.1.20"
/experiment="EXISTENCE:direct assay:GO:0004489
methylenetetrahydrofolate reductase [NAD(P)H] activity
[PMID:10600168]"
/experiment="EXISTENCE:direct assay:GO:0035999
tetrahydrofolate interconversion [PMID:10600168]"
/note="Protein with MTHFR activity in vitro; null mutant
has no phenotype and is prototrophic for methionine; MET13
encodes major isozyme of methylenetetrahydrofolate
reductase (MTHFR)"
/codon_start=1
/product="methylenetetrahydrofolate reductase (NAD(P)H)
MET12"
/protein_id="NP_015302.1"
/db_xref="GeneID:856084"
/db_xref="SGD:S000005944"
/translation="MSIRDLYHARASPFISLEFFPPKTELGTRNLMERMHRMTALDPL
FITVTWGAGGTTAEKTLTLASLAQQTLNIPVCMHLTCTNTEKAIIDDALDRCYNAGIR
NILALRGDPPIGEDWLDSQSNESPFKYAVDLVRYIKQSYGDKFCVGVAAYPEGHCEGE
AEGHEQDPLKDLVYLKEKVEAGADFVITQLFYDVEKFLTFEMLFRERISQDLPLFPGL
MPINSYLLFHRAAKLSHASIPPAILSRFPPEIQSDDNAVKSIGVDILIELIQEIYQRT
SGRIKGFHFYTLNLEKAIAQIVSQSPVLSHIVNESSEEEGEDETSGEIGSIENVPIED
ADGDIVLDDSNEETVANRKRRRHSSLDSAKLIFNRAIVTEKGLRYNNENGSMPSKKAL
ISISKGHGTLGRDATWDEFPNGRFGDSRSPAYGEIDGYGPSIKVSKSKALELWGIPKT
IGDLKDIFIKYLEGSTDAIPWSDLGLSAETALIQEELIQLNYRGYLTLASQPATNATL
SSDKIFGWGPAKGRLYQKAFVEMFIHRQQWETTLKPKLDHYGRRKFSYYAGDSSGSFE
TNLDPHSSSVVTWGVFPNSPVKQTTIIEEESFKAWRDEAFSIWSEWAKLFPRNTPANI
LLRLVHKDYCLVSIVHHDFKETDELWEMLLDQA"
gene <506697..>509999
/gene="RAD1"
/locus_tag="YPL022W"
/gene_synonym="LPB9; RAD12"
/db_xref="GeneID:856085"
mRNA <506697..>509999
/gene="RAD1"
/locus_tag="YPL022W"
/gene_synonym="LPB9; RAD12"
/product="ssDNA endodeoxyribonuclease RAD1"
/transcript_id="NM_001183836.1"
/db_xref="GeneID:856085"
CDS 506697..509999
/gene="RAD1"
/locus_tag="YPL022W"
/gene_synonym="LPB9; RAD12"
/experiment="EXISTENCE:direct assay:GO:0000014
single-stranded DNA endodeoxyribonuclease activity
[PMID:8253764]"
/experiment="EXISTENCE:direct assay:GO:0003697
single-stranded DNA binding [PMID:8253764]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9852079]"
/experiment="EXISTENCE:direct assay:GO:0006289
nucleotide-excision repair [PMID:7559571]"
/experiment="EXISTENCE:mutant phenotype:GO:0000710 meiotic
mismatch repair [PMID:11514439]"
/experiment="EXISTENCE:mutant phenotype:GO:0000735 removal
of nonhomologous ends [PMID:7891718|PMID:1411547]"
/experiment="EXISTENCE:mutant phenotype:GO:0000736
double-strand break repair via single-strand annealing,
removal of nonhomologous ends [PMID:7891718|PMID:1411547]"
/experiment="EXISTENCE:mutant phenotype:GO:0006277 DNA
amplification [PMID:23271978]"
/experiment="EXISTENCE:mutant phenotype:GO:0006312 mitotic
recombination [PMID:3065620]"
/experiment="EXISTENCE:physical interaction:GO:0000110
nucleotide-excision repair factor 1 complex
[PMID:8621533]"
/note="Single-stranded DNA endonuclease (with Rad10p);
cleaves single-stranded DNA during nucleotide excision
repair and double-strand break repair; subunit of
Nucleotide Excision Repair Factor 1 (NEF1); homolog of
human XPF protein"
/codon_start=1
/product="ssDNA endodeoxyribonuclease RAD1"
/protein_id="NP_015303.1"
/db_xref="GeneID:856085"
/db_xref="SGD:S000005943"
/translation="MSQLFYQGDSDDELQEELTRQTTQASQSSKIKNEDEPDDSNHLN
EVENEDSKVLDDDAVLYPLIPNEPDDIETSKPNINDIRPVDIQLTLPLPFQQKVVENS
LITEDALIIMGKGLGLLDIVANLLHVLATPTSINGQLKRALVLVLNAKPIDNVRIKEA
LEELSWFSNTGKDDDDTAVESDDELFERPFNVVTADSLSIEKRRKLYISGGILSITSR
ILIVDLLSGIVHPNRVTGMLVLNADSLRHNSNESFILEIYRSKNTWGFIKAFSEAPET
FVMEFSPLRTKMKELRLKNVLLWPRFRVEVSSCLNATNKTSHNKVIEVKVSLTNSMSQ
IQFGLMECLKKCIAELSRKNPELALDWWNMENVLDINFIRSIDSVMVPNWHRISYESK
QLVKDIRFLRHLLKMLVTSDAVDFFGEIQLSLDANKPSVSRKYSESPWLLVDEAQLVI
SYAKKRIFYKNEYTLEENPKWEQLIHILHDISHERMTNHLQGPTLVACSDNLTCLELA
KVLNASNKKRGVRQVLLNKLKWYRKQREETKKLVKEVQSQDTFPENATLNVSSTFSKE
QVTTKRRRTRGASQVAAVEKLRNAGTNVDMEVVFEDHKLSEEIKKGSGDDLDDGQEEN
AANDSKIFEIQEQENEILIDDGDAEFDNGELEYVGDLPQHITTHFNKDLWAEHCNEYE
YVDRQDEILISTFKSLNDNCSLQEMMPSYIIMFEPDISFIRQIEVYKAIVKDLQPKVY
FMYYGESIEEQSHLTAIKREKDAFTKLIRENANLSHHFETNEDLSHYKNLAERKLKLS
KLRKSNTRNAGGQQGFHNLTQDVVIVDTREFNASLPGLLYRYGIRVIPCMLTVGDYVI
TPDICLERKSISDLIGSLQNNRLANQCKKMLKYYAYPTLLIEFDEGQSFSLEPFSERR
NYKNKDISTVHPISSKLSQDEIQLKLAKLVLRFPTLKIIWSSSPLQTVNIILELKLGR
EQPDPSNAVILGTNKVRSDFNSTAKGLKDGDNESKFKRLLNVPGVSKIDYFNLRKKIK
SFNKLQKLSWNEINELINDEDLTDRIYYFLRTEKEEQEQESTDENLESPGKTTDDNAL
HDHHNDVPEAPV"
gene <511101..>511664
/gene="ECM23"
/locus_tag="YPL021W"
/gene_synonym="SRD2"
/db_xref="GeneID:856086"
mRNA <511101..>511664
/gene="ECM23"
/locus_tag="YPL021W"
/gene_synonym="SRD2"
/product="Ecm23p"
/transcript_id="NM_001183835.1"
/db_xref="GeneID:856086"
CDS 511101..511664
/gene="ECM23"
/locus_tag="YPL021W"
/gene_synonym="SRD2"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:19111667]"
/experiment="EXISTENCE:mutant phenotype:GO:0007124
pseudohyphal growth [PMID:11889490]"
/note="Non-essential protein of unconfirmed function;
affects pre-rRNA processing, may act as a negative
regulator of the transcription of genes involved in
pseudohyphal growth; homologous to Srd1p"
/codon_start=1
/product="Ecm23p"
/protein_id="NP_015304.1"
/db_xref="GeneID:856086"
/db_xref="SGD:S000005942"
/translation="MLYNKEQGTSGASSSGRRTKFHFDRFVQMVLFIAANPNYCCSVA
SIPKSGVTPDLKRADILEQKIKSLNSALSPKLKEESRLGGPLHNPSILPAPSFSSLPI
SSNGKKSLAGYRPKSRKKQTILPNGQPKECATCGDTWTSQWRSGPNGNVELCSRCGIA
YRKKMEKKIRSQQSSDDGTKNFIFKNK"
rep_origin 511636..511793
/note="ARS1621; Autonomously Replicating Sequence"
/db_xref="SGD:S000118417"
gene complement(<512313..>514178)
/gene="ULP1"
/locus_tag="YPL020C"
/gene_synonym="NIB1"
/db_xref="GeneID:856087"
mRNA complement(<512313..>514178)
/gene="ULP1"
/locus_tag="YPL020C"
/gene_synonym="NIB1"
/product="SUMO protease ULP1"
/transcript_id="NM_001183834.1"
/db_xref="GeneID:856087"
CDS complement(512313..514178)
/gene="ULP1"
/locus_tag="YPL020C"
/gene_synonym="NIB1"
/EC_number="3.4.22.68"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:20647537|PMID:10713161]"
/experiment="EXISTENCE:direct assay:GO:0005643 nuclear
pore [PMID:12471376]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:20647537]"
/experiment="EXISTENCE:direct assay:GO:0008234
cysteine-type peptidase activity [PMID:10094048]"
/experiment="EXISTENCE:direct assay:GO:0016926 protein
desumoylation [PMID:10094048]"
/experiment="EXISTENCE:direct assay:GO:0016929 deSUMOylase
activity [PMID:10094048]"
/experiment="EXISTENCE:mutant phenotype:GO:0000086 G2/M
transition of mitotic cell cycle [PMID:10094048]"
/experiment="EXISTENCE:mutant phenotype:GO:0016926 protein
desumoylation [PMID:10094048]"
/experiment="EXISTENCE:mutant phenotype:GO:0016929
deSUMOylase activity [PMID:10094048]"
/note="Protease that specifically cleaves Smt3p protein
conjugates; required for cell cycle progression;
associates with nucleoporins and may interact with septin
rings during telophase; contributes to regulation of
telomeric silencing; localizes to the nuclear pore
channel; sequestered to the nucleolus under stress
conditions"
/codon_start=1
/product="SUMO protease ULP1"
/protein_id="NP_015305.1"
/db_xref="GeneID:856087"
/db_xref="SGD:S000005941"
/translation="MSVEVDKHRNTLQYHKKNPYSPLFSPISTYRCYPRVLNNPSESR
RSASFSGIYKKRTNTSRFNYLNDRRVLSMEESMKDGSDRASKAGFIGGIRETLWNSGK
YLWHTFVKNEPRNFDGSEVEASGNSDVESRSSGSRSSDVPYGLRENYSSDTRKHKFDT
STWALPNKRRRIESEGVGTPSTSPISSLASQKSNCDSDNSITFSRDPFGWNKWKTSAI
GSNSENNTSDQKNSYDRRQYGTAFIRKKKVAKQNINNTKLVSRAQSEEVTYLRQIFNG
EYKVPKILKEERERQLKLMDMDKEKDTGLKKSIIDLTEKIKTILIENNKNRLQTRNEN
DDDLVFVKEKKISSLERKHKDYLNQKLKFDRSILEFEKDFKRYNEILNERKKIQEDLK
KKKEQLAKKKLVPELNEKDDDQVQKALASRENTQLMNRDNIEITVRDFKTLAPRRWLN
DTIIEFFMKYIEKSTPNTVAFNSFFYTNLSERGYQGVRRWMKRKKTQIDKLDKIFTPI
NLNQSHWALGIIDLKKKTIGYVDSLSNGPNAMSFAILTDLQKYVMEESKHTIGEDFDL
IHLDCPQQPNGYDCGIYVCMNTLYGSADAPLDFDYKDAIRMRRFIAHLILTDALK"
gene complement(<514511..>517018)
/gene="VTC3"
/locus_tag="YPL019C"
/gene_synonym="PHM2"
/db_xref="GeneID:856088"
mRNA complement(<514511..>517018)
/gene="VTC3"
/locus_tag="YPL019C"
/gene_synonym="PHM2"
/product="vacuolar transporter chaperone"
/transcript_id="NM_001183833.1"
/db_xref="GeneID:856088"
CDS complement(514511..517018)
/gene="VTC3"
/locus_tag="YPL019C"
/gene_synonym="PHM2"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane
[PMID:11823419|PMID:11102525|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005516 calmodulin
binding [PMID:17079729]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:17079729]"
/experiment="EXISTENCE:direct assay:GO:0007034 vacuolar
transport [PMID:17079729]"
/experiment="EXISTENCE:direct assay:GO:0016237
microautophagy [PMID:17079729]"
/experiment="EXISTENCE:genetic interaction:GO:0006797
polyphosphate metabolic process [PMID:11102525]"
/experiment="EXISTENCE:mutant phenotype:GO:0006797
polyphosphate metabolic process [PMID:11102525]"
/experiment="EXISTENCE:mutant phenotype:GO:0007034
vacuolar transport [PMID:17079729]"
/experiment="EXISTENCE:mutant phenotype:GO:0016237
microautophagy [PMID:17079729]"
/experiment="EXISTENCE:mutant phenotype:GO:0042144 vacuole
fusion, non-autophagic [PMID:11823419]"
/experiment="EXISTENCE:physical interaction:GO:0033254
vacuolar transporter chaperone complex [PMID:11823419]"
/note="Regulatory subunit of vacuolar transporter
chaperone (VTC) complex; involved in membrane trafficking,
vacuolar polyphosphate accumulation, microautophagy and
non-autophagic vacuolar fusion; important regulator of
substrate invagination from the vacuolar membrane;
targeted to vacuole via AP-3 pathway; VTC3 has a paralog,
VTC2, that arose from the whole genome duplication"
/codon_start=1
/product="vacuolar transporter chaperone"
/protein_id="NP_015306.1"
/db_xref="GeneID:856088"
/db_xref="SGD:S000005940"
/translation="MLFGIKLANDVYPPWKDSYIDYERLKKLLKESVIHDGRSSVDSW
SERNESDFVEALDKELEKVYTFQISKYNAVLRKLDDLEENTKSAEKIQKINSEQFKNT
LEECLDEAQRLDNFDRLNFTGFIKIVKKHDKLHPNYPSVKSLLQVRLKELPFNNSEEY
SPLLYRISYLYEFLRSNYDHPNTVSKSLASTSKLSHFSNLEDASFKSYKFWVHDDNIM
EVKARILRHLPALVYASVPNENDDFVDNLESDVRVQPEARLNIGSKSNSLSSDGNSNQ
DVEIGKSKSVIFPQSYDPTITTLYFDNDFFDLYNNRLLKISGAPTLRLRWIGKLLDKP
DIFLEKRTFTENTETGNSSFEEIRLQMKAKFINNFIFKNDPSYKNYLINQLRERGTQK
EELEKLSRDFDNIQNFIVEEKLQPVLRATYNRTAFQIPGDQSIRVTIDSNIMYIREDS
LDKNRPIRNPENWHRDDIDSNIPNPLRFLRAGEYSKFPYSVMEIKVINQDNSQMPNYE
WIKDLTNSHLVNEVPKFSLYLQGVASLFGEDDKYVNILPFWLPDLETDIRKNPQEAYE
EEKKTLQKQKSIHDKLDNMRRLSKISVPDGKTTERQGQKDQNTRHVIADLEDHESSDE
EGTALPKKSAVKKGKKFKTNAAFLKILAGKNISENGNDPYSDDTDSASSFQLPPGVKK
PVHLLKNAGPVKVEAKVWLANERTFNRWLSVTTLLSVLTFSIYNSVQKAEFPQLADLL
AYVYFFLTLFCGVWAYRTYLKRLTLIKGRSGKHLDAPVGPILVAVVLIVTLVVNFSVA
FKEAARRERGLVNVSSQPSLPRTLKPIQDFIFNLVGE"
gene <517651..>518760
/gene="CTF19"
/locus_tag="YPL018W"
/gene_synonym="MCM18"
/db_xref="GeneID:856089"
mRNA <517651..>518760
/gene="CTF19"
/locus_tag="YPL018W"
/gene_synonym="MCM18"
/product="Ctf19p"
/transcript_id="NM_001183832.1"
/db_xref="GeneID:856089"
CDS 517651..518760
/gene="CTF19"
/locus_tag="YPL018W"
/gene_synonym="MCM18"
/experiment="EXISTENCE:direct assay:GO:0000776 kinetochore
[PMID:10189365]"
/experiment="EXISTENCE:direct assay:GO:0000817 COMA
complex [PMID:14633972]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10189365]"
/experiment="EXISTENCE:direct assay:GO:0008608 attachment
of spindle microtubules to kinetochore [PMID:10189365]"
/experiment="EXISTENCE:mutant phenotype:GO:0007059
chromosome segregation [PMID:10189365]"
/experiment="EXISTENCE:mutant phenotype:GO:0007094 mitotic
spindle assembly checkpoint signaling [PMID:22426531]"
/experiment="EXISTENCE:mutant phenotype:GO:0008608
attachment of spindle microtubules to kinetochore
[PMID:10189365]"
/experiment="EXISTENCE:mutant phenotype:GO:0034087
establishment of mitotic sister chromatid cohesion
[PMID:19730685]"
/experiment="EXISTENCE:mutant phenotype:GO:0071459 protein
localization to chromosome, centromeric region
[PMID:26653857]"
/note="Outer kinetochore protein, needed for accurate
chromosome segregation; component of kinetochore
sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that
functions as platform for kinetochore assembly; required
for spindle assembly checkpoint; minimizes potentially
deleterious centromere-proximal crossovers by preventing
meiotic DNA break formation proximal to centromere;
homolog of human centromere constitutive-associated
network (CCAN) subunit CENP-P and fission yeast fta2"
/codon_start=1
/product="Ctf19p"
/protein_id="NP_015307.1"
/db_xref="GeneID:856089"
/db_xref="SGD:S000005939"
/translation="MDFTSDTTNSHDTSNSHLSLEDAVGTHHAGEADVNIDGDEKQQL
SLLDDDQVRALKLQEEKDALLTRRNTLLQEIQTYQNILMKENNSKTKNGDILQNDITQ
DFLNLISISSSNPNSAISDRKRVERINGLTNLQKELVTKYDTLPLLNMNLRLSYLRDH
TYPHLQVSVQSRDRVHNDGIEVLVVNYKFCRNTMNPFEIQFKMFYKFEDSTLLKWEIL
RISTNVRLKAKQLLATRNFQKCLLSLYEFDKIKSKKTGIFQNLINLLKRKTRCYLMNN
SDSLIVERVIREGRLTTIKLQINFIITMPGERGKPRNCFLPMSKISIALWKGGERFNQ
IDLDEICYGLIKEYGVKTGLKEICNVCLFPDMYAR"
gene complement(<518734..>520233)
/gene="IRC15"
/locus_tag="YPL017C"
/db_xref="GeneID:856090"
mRNA complement(<518734..>520233)
/gene="IRC15"
/locus_tag="YPL017C"
/product="Irc15p"
/transcript_id="NM_001183831.1"
/db_xref="GeneID:856090"
CDS complement(518734..520233)
/gene="IRC15"
/locus_tag="YPL017C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005874 microtubule
[PMID:19285398]"
/experiment="EXISTENCE:direct assay:GO:0007020 microtubule
nucleation [PMID:19285398]"
/experiment="EXISTENCE:direct assay:GO:0008017 microtubule
binding [PMID:19285398]"
/experiment="EXISTENCE:direct assay:GO:0016651
oxidoreductase activity, acting on NAD(P)H
[PMID:30267443]"
/experiment="EXISTENCE:mutant phenotype:GO:0006312 mitotic
recombination [PMID:18085829]"
/experiment="EXISTENCE:mutant phenotype:GO:0034453
microtubule anchoring [PMID:19285398]"
/experiment="EXISTENCE:mutant phenotype:GO:0045144 meiotic
sister chromatid segregation [PMID:18069899]"
/experiment="EXISTENCE:mutant phenotype:GO:0045931
positive regulation of mitotic cell cycle [PMID:19285398]"
/experiment="EXISTENCE:mutant phenotype:GO:0051315
attachment of mitotic spindle microtubules to kinetochore
[PMID:19285398]"
/note="Microtubule associated protein; regulates
microtubule dynamics; required for accurate meiotic
chromosome segregation; null mutant displays large budded
cells due to delayed mitotic progression, increased levels
of spontaneous Rad52 foci; IRC15 has a paralog, LPD1, that
arose from the whole genome duplication"
/codon_start=1
/product="Irc15p"
/protein_id="NP_015308.1"
/db_xref="GeneID:856090"
/db_xref="SGD:S000005938"
/translation="MGGEDEILSTMEDFAAVYDVLVIGCGPGGFTAAMQASQAGLLTA
CVDQRASLGGAYLVDGAVPSKTLLYESYLYRLLQQQELIEQRGTRLFPAKFDMQAAQS
ALKHNIEELGNVYKRELSKNNVTVYKGTAAFKDPHHVEIAQRGMKPFIVEAKYIVVAT
GSAVIQCPGVAIDNDKIISSDKALSLDYIPSRFTIMGGGTIGLEIACIFNNLGSRVTI
VESQSEICQNMDNELASATKTLLQCQGIAFLLDTRVQLAEADAAGQLNITLLNKVSKK
TYVHHCDVLMVSIGRRPLLKGLDISSIGLDERDFVENVDVQTQSLLKYPHIKPIGDVT
LGPMLALKAEEQAIRAIQSIGCTGSDGTSNCGFPPNVLYCQPQIGWVGYTEEGLAKAR
IPYQKGRVLFSQNVRYNTLLPREENTTVSPFIKVLIDSRDMKILGVHMINDDANELLS
QASMAVSLGLTAHDVCKVPFPHPSLSESFKQAVQLAMANGTSPGVHVRE"
gene <521014..>524958
/gene="SWI1"
/locus_tag="YPL016W"
/gene_synonym="ADR6; GAM3; LPA1"
/db_xref="GeneID:856091"
mRNA <521014..>524958
/gene="SWI1"
/locus_tag="YPL016W"
/gene_synonym="ADR6; GAM3; LPA1"
/product="Swi1p"
/transcript_id="NM_001183830.2"
/db_xref="GeneID:856091"
CDS 521014..524958
/gene="SWI1"
/locus_tag="YPL016W"
/gene_synonym="ADR6; GAM3; LPA1"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:3143101]"
/experiment="EXISTENCE:direct assay:GO:0006338 chromatin
remodeling [PMID:8016655]"
/experiment="EXISTENCE:direct assay:GO:0016514 SWI/SNF
complex
[PMID:18644858|PMID:8127913|PMID:8016655|PMID:8159677]"
/experiment="EXISTENCE:mutant phenotype:GO:0006261
DNA-templated DNA replication [PMID:10198436]"
/experiment="EXISTENCE:mutant phenotype:GO:0016514 SWI/SNF
complex [PMID:8159677]"
/experiment="EXISTENCE:mutant phenotype:GO:0031496
positive regulation of mating type switching
[PMID:6436497]"
/experiment="EXISTENCE:mutant phenotype:GO:0034198
cellular response to amino acid starvation
[PMID:10549298]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:8016655|PMID:3542227|PMID:10549298|PMID:1339306]"
/experiment="EXISTENCE:mutant phenotype:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:14580348]"
/experiment="EXISTENCE:physical interaction:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:11865042|PMID:14580348]"
/note="Subunit of the SWI/SNF chromatin remodeling
complex; regulates transcription by remodeling chromatin;
required for transcription of many genes, including ADH1,
ADH2, GAL1, HO, INO1 and SUC2; self-assembles to form
[SWI+] prion and to alter expression pattern; human
homolog ARID1A is a candidate tumor suppressor gene in
breast cancer"
/codon_start=1
/product="Swi1p"
/protein_id="NP_015309.1"
/db_xref="GeneID:856091"
/db_xref="SGD:S000005937"
/translation="MDFFNLNNNNNNNNTTTTTTTTNNNNTNNNNTNNNNNPANNTNN
NNSTGHSSNTNNNTNNNNTNTGASGVDDFQNFFDPKPFDQNLDSNNNNSNSNNNDNNN
SNTVASSTNFTSPTAVVNNAAPANVTGGKAANFIQNQSPQFNSPYDSNNSNTNLNSLS
PQAILAKNSIIDSSNLPLQAQQQLYGGNNNNNSTGIANDNVITPHFITNVQSISQNSS
SSTPNTNSNSTPNANQQFLPFNNSASNNGNLTSNQLISNYAASNSMDRSSSASNEFVP
NTSDNNNNSNNHNMRNNSNNKTSNNNNVTAVPAATPANTNNSTSNANTVFSERAAMFA
ALQQKQQQRFQALQQQQQQQQNQQQQNQQPQQQQQQQQNPKFLQSQRQQQQRSILQSL
NPALQEKISTELNNKQYELFMKSLIENCKKRNMPLQSIPEIGNRKINLFYLYMLVQKF
GGADQVTRTQQWSMVAQRLQISDYQQLESIYFRILLPYERHMISQEGIKETQAKRIFL
QQFLQELLKKVQQQQQAAALANANNNINSASSAPTPAAPGASVPATAAPGTEAGIVPV
SANTPKSLNSNININVNNNNIGQQQVKKPRKQRVKKKTKKELELERKEREDFQKRQQK
LLEDQQRQQKLLLETKLRQQYEIELKKLPKVYKRSIVRNYKPLINRLKHYNGYDINYI
SKIGEKIDSNKPIFLFAPELGAINLHALSMSLQSKNLGEINTALNTLLVTSADSNLKI
SLVKYPELLDSLAILGMNLLSNLSQNVVPYHRNTSDYYYEDAGSNQYYVTQHDKMVDK
IFEKVNNNATLTPNDSNDEKVTILVDSLTGNQLPTPTPTEMEPDLDTECFISMQSTSP
AVKQWDLLPEPIRFLPNQFPLKIHRTPYLTSLKKIKDEIDDPFTKINTRGAEDPKVLI
NDQLSTISMILRNISFSDNNSRIMSRNFYLKRFISDLLWLVLIHPENFTCNRKILNFK
KDLVIVLSNISHLLEIASSIDCLLILILVISFGQPKLNPMASSSSFGSESLTFNEFQL
QWGKYQTFGVDILAKLFSLEKPNLNYFKSILLNKNTGNNLYDRNSNNNHKDKKLLRRL
LNLYNDNNKNNNNRHNLLNDVVSFLFSAIPLQQVLSQSADPSLLIDQFSPVISQSLTS
ILVIVQKILPLSNEVFEISENNSDSNSNNNGNKDSSFNFNKNLPFVWLSSEENIGSGL
LKLSEIILNINNSTSKNTLLQQQNYSKVLLPSINISCVQLIKCLVEKSICFENCLNND
PEILKKIASIPNLFPTDLEIFQLFTNPSVDIQIINQYQLLYNLKNDILTNLE"
gene complement(<525810..>526883)
/gene="HST2"
/locus_tag="YPL015C"
/db_xref="GeneID:856092"
mRNA complement(<525810..>526883)
/gene="HST2"
/locus_tag="YPL015C"
/product="histone deacetylase HST2"
/transcript_id="NM_001183829.1"
/db_xref="GeneID:856092"
CDS complement(525810..526883)
/gene="HST2"
/locus_tag="YPL015C"
/EC_number="2.3.1.286"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:17110954]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11226170]"
/experiment="EXISTENCE:direct assay:GO:0017136 histone
deacetylase activity, NAD-dependent [PMID:10811920]"
/experiment="EXISTENCE:genetic interaction:GO:0045950
negative regulation of mitotic recombination
[PMID:16051752]"
/experiment="EXISTENCE:mutant phenotype:GO:0000183 rDNA
heterochromatin formation [PMID:16051752|PMID:11226170]"
/experiment="EXISTENCE:mutant phenotype:GO:0017136 histone
deacetylase activity, NAD-dependent [PMID:10841563]"
/experiment="EXISTENCE:mutant phenotype:GO:0045950
negative regulation of mitotic recombination
[PMID:16051752]"
/note="Cytoplasmic NAD(+)-dependent protein deacetylase;
deacetylation targets are primarily cytoplasmic proteins;
member of the silencing information regulator 2 (Sir2)
family of NAD(+)-dependent protein deacetylases; modulates
nucleolar (rDNA) and telomeric silencing; possesses
NAD(+)-dependent histone deacetylase activity in vitro;
contains a nuclear export signal (NES); function regulated
by its nuclear export"
/codon_start=1
/product="histone deacetylase HST2"
/protein_id="NP_015310.1"
/db_xref="GeneID:856092"
/db_xref="SGD:S000005936"
/translation="MSVSTASTEMSVRKIAAHMKSNPNAKVIFMVGAGISTSCGIPDF
RSPGTGLYHNLARLKLPYPEAVFDVDFFQSDPLPFYTLAKELYPGNFRPSKFHYLLKL
FQDKDVLKRVYTQNIDTLERQAGVKDDLIIEAHGSFAHCHCIGCGKVYPPQVFKSKLA
EHPIKDFVKCDVCGELVKPAIVFFGEDLPDSFSETWLNDSEWLREKITTSGKHPQQPL
VIVVGTSLAVYPFASLPEEIPRKVKRVLCNLETVGDFKANKRPTDLIVHQYSDEFAEQ
LVEELGWQEDFEKILTAQGGMGDNSKEQLLEIVHDLENLSLDQSEHESADKKDKKLQR
LNGHDSDEDGASNSSSSQKAAKE"
gene <527547..>528692
/gene="CIP1"
/locus_tag="YPL014W"
/db_xref="GeneID:856093"
mRNA <527547..>528692
/gene="CIP1"
/locus_tag="YPL014W"
/product="Cip1p"
/transcript_id="NM_001183828.1"
/db_xref="GeneID:856093"
CDS 527547..528692
/gene="CIP1"
/locus_tag="YPL014W"
/experiment="EXISTENCE:direct assay:GO:0004861
cyclin-dependent protein serine/threonine kinase inhibitor
activity [PMID:27005485]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0000082 G1/S
transition of mitotic cell cycle [PMID:27005485]"
/experiment="EXISTENCE:genetic interaction:GO:0001933
negative regulation of protein phosphorylation
[PMID:27005485]"
/experiment="EXISTENCE:mutant phenotype:GO:0000082 G1/S
transition of mitotic cell cycle [PMID:27005485]"
/experiment="EXISTENCE:mutant phenotype:GO:0001933
negative regulation of protein phosphorylation
[PMID:27005485]"
/note="Cyclin-dependent kinase inhibitor; activated by
environmental stress; interacts with and inhibits the
Cdc28p/Cln2p, G1/S phase cyclin-dependent kinase complex
but not S-phase, or M-phase complexes; overexpression
blocks cells in G1 phase and stabilizes the Cdc28p
inhibitor Sic1p, while disruption accelerates the G1/S
phase transition; phosphorylated during S phase in a
Cdc28p-dependent manner; green fluorescent protein
(GFP)-fusion protein localizes to the cytoplasm and to the
nucleus"
/codon_start=1
/product="Cip1p"
/protein_id="NP_015311.1"
/db_xref="GeneID:856093"
/db_xref="SGD:S000005935"
/translation="MLLERLHKRLHAGSSRRSQENKDKNCKPEDALPIQPEAQHQTQD
PQPLLNCDYDDMIAFDRNLSTPVFTPVMTPINNSSSNQAKSSDASYFPPYLNANRTRQ
NSASSLASSVSDFAQNFKQHTFYNNNAQFTSFTPQFVGLLLEVYQNTCSDPTITPFDT
TNPPSGILNRVAKAAIQQSELQQLDIGCDRNSWLLTLVRQRLLQEVRKDGYLSRNTSL
TSLPPPPPPQFSEMLRVPSPFVNADITDPIPLSNTNSNPNVSSTTSLTNTLNWYSLQR
SNVSMKNRNGSSQYISELQPQPILARTNSNNSVSNSNAFSLLTPTPTTDSAFNFNIAL
LSRQRSNIISSPLASTRLPTANVSTEESSILPNESLKLKRDLLRLKR"
gene complement(<528985..>529350)
/gene="MRPS16"
/locus_tag="YPL013C"
/gene_synonym="bS16m"
/db_xref="GeneID:856094"
mRNA complement(<528985..>529350)
/gene="MRPS16"
/locus_tag="YPL013C"
/gene_synonym="bS16m"
/product="mitochondrial 37S ribosomal protein bS16m
MRPS16"
/transcript_id="NM_001183827.1"
/db_xref="GeneID:856094"
CDS complement(528985..529350)
/gene="MRPS16"
/locus_tag="YPL013C"
/gene_synonym="bS16m"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:12392552]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:12392552]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005763
mitochondrial small ribosomal subunit [PMID:12392552]"
/note="Mitochondrial ribosomal protein of the small
subunit"
/codon_start=1
/product="mitochondrial 37S ribosomal protein bS16m
MRPS16"
/protein_id="NP_015312.1"
/db_xref="GeneID:856094"
/db_xref="SGD:S000005934"
/translation="MTCGLVRIRLARFGRKNSPVYNIVVANSRKARDAKPIEVLGTYV
PVPSPVTKRELKRGVVPIKDVKLDFDRTKYWIGVGAQPSETVTKLLRKAGILNDAWAT
SKNSNVNRKVVFERMETLE"
gene <529723..>533409
/gene="RRP12"
/locus_tag="YPL012W"
/db_xref="GeneID:856095"
mRNA <529723..>533409
/gene="RRP12"
/locus_tag="YPL012W"
/product="mRNA-binding protein RRP12"
/transcript_id="NM_001183826.1"
/db_xref="GeneID:856095"
CDS 529723..533409
/gene="RRP12"
/locus_tag="YPL012W"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14729571]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/note="Protein required for export of the ribosomal
subunits; associates with the RNA components of the
pre-ribosomes; has a role in nuclear import in association
with Pse1p; also plays a role in the cell cycle and the
DNA damage response; contains HEAT-repeats"
/codon_start=1
/product="mRNA-binding protein RRP12"
/protein_id="NP_015313.1"
/db_xref="GeneID:856095"
/db_xref="SGD:S000005933"
/translation="MDQDKVAFLLELEDKLAKIRSQVNSKLENQKHIAIILTAVEENI
AGQATNDVSKNIVNYIISFMSLLDQAVDPSTHEIKDIQLASSSTYLLDLIFHYSPKVL
LRSKFSEILTKIAPCITAEKANAPLIRAAIGCLESLLIAQDAQAWNNTYDLNVTPKRG
LQGILELSLDVRPKVRKRALDAVHAVLLNPPVAPTAEHVAAVFVADFCDKQLAGILND
LSNLSNKQLKAQKTKEDINASVMRSLRLITSVVSTGQWPSSQIEPLCDVLLGVTKSSE
QYLVSASFECFESMFKTMAETTISSGLAENKYLRVLDTIFALKPSNVDTLLTKSWIAV
VIKGMSTYATHQPLKALRKIPGVFHIMCTYLASETPEVYQAASQCLISILSESVKDDL
LLYTPSVDEKVFKNVDEIISQIAKTFIDFLSIRYSHCSREILKILVAAFNKFRYRSNP
HFLKSLKIVDTWRVNEEQFMDLRNEIELVIGASISAMGPEMILAEAPLNLDNPSSERP
GRAWLLPLIRDYTKNANLATFQNELAPYIKSFQSKFDKVPEESIQLRVFQTIVDQIWS
TLPRFCELPMDLRESFTDEFASELSSLLYSEVELRTTICHALKVLAESNVSYAEESSS
HNVLLLQRFPISEAQKNIEYLSTKSTNLLAVLFNVYTQTTPNARSYILETIDQYLKIT
SKEDLEKTFNNVCGLLKNSMNEESSGNVNKEKKKPQLTATLLDLIICMITYLPVSSYS
ALFSMFSLTVNSADALIQKRAYRIITKLSELKSGSTAVAQFISDIENVMVDSASSVQT
SAKAARLTAIKTIVELLPLDHLDFIVRTVAEVILSTKDVNEKSRETAFDTLICMGRKM
NEPNGIIKLFQIPGYDPTTPDQSSSISEFFKIISAGLIGESQHMVSSSITGYACLVFE
FKNELDSGILMDIYDTIELYLTSNSREIVKSAIGFTKVCVLGLPEELMRPKVPELLLK
LLRWSHEHTGHFKAKVKHIIERLIRRFGYDYIEANFPEEDRRLLTNIRKMRNRNKRKD
EEVTTGVSDVAATKGSRFMSAFDEAVYGSDEENDNGSDQEENVAGGKMKNGAKQFIVE
SGDNPLDLLDSQTLAHISSTRPKKFNKNQNRARFNDDAFNFDSEGKLVVKGQPKPSTN
VDDPLSAVTSGINAYLEAVKSGPVRGQRNKLKFRKNGKDSDEFGDDDDGEKDSRLMRG
RVNQGNKIGKHNKKGPKFKSRKKL"
gene complement(<533642..>534703)
/gene="TAF3"
/locus_tag="YPL011C"
/gene_synonym="TAF47"
/db_xref="GeneID:856096"
mRNA complement(<533642..>534703)
/gene="TAF3"
/locus_tag="YPL011C"
/gene_synonym="TAF47"
/product="Taf3p"
/transcript_id="NM_001183825.1"
/db_xref="GeneID:856096"
CDS complement(533642..534703)
/gene="TAF3"
/locus_tag="YPL011C"
/gene_synonym="TAF47"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:17660549]"
/experiment="EXISTENCE:direct assay:GO:0005669
transcription factor TFIID complex
[PMID:10788514|PMID:15448131]"
/experiment="EXISTENCE:direct assay:GO:0006366
transcription by RNA polymerase II
[PMID:15448131|PMID:12138208]"
/note="TFIID subunit (47 kDa); involved in promoter
binding and RNA polymerase II transcription initiation"
/codon_start=1
/product="Taf3p"
/protein_id="NP_015314.1"
/db_xref="GeneID:856096"
/db_xref="SGD:S000005932"
/translation="MTTNNDFYFALLRISILQLLKAQGFDRARPSLVDVMTDLYAKFL
SLLASEVSSIAQARCDQDDTIALQDITLALENLGIVKPTNVLDVYDENSELSSSRGME
KFKDWCIYSTQLTDARITALPTVELLQSEEKESDPLSAIPDYLNQLLQNKGAKQKLET
KNRKTELIEDLINNNGLDDWIKLVIARQRINMIERASKKESQNVPALPHIAGYKSSIL
SRHHHTTITNEDRMPSAMTPRDEDALTEIQENPFVTSKLPIMRKENRLENITLSFEDE
ELESLGEVEGPNQKSQENNNEESFKENNKSLTESPHGDDRDISMFQFDSNVDTKWAEQ
EDMDSTFQRRTSLDYGGYF"
gene <535018..>535587
/gene="RET3"
/locus_tag="YPL010W"
/db_xref="GeneID:856097"
mRNA <535018..>535587
/gene="RET3"
/locus_tag="YPL010W"
/product="coatomer subunit zeta"
/transcript_id="NM_001183824.1"
/db_xref="GeneID:856097"
CDS 535018..535587
/gene="RET3"
/locus_tag="YPL010W"
/experiment="EXISTENCE:direct assay:GO:0030126 COPI
vesicle coat [PMID:21435344]"
/experiment="EXISTENCE:mutant phenotype:GO:0006890
retrograde vesicle-mediated transport, Golgi to
endoplasmic reticulum [PMID:8617224]"
/experiment="EXISTENCE:mutant phenotype:GO:0030126 COPI
vesicle coat [PMID:8617224]"
/note="Zeta subunit of the coatomer complex (COPI); COPI
coats Golgi-derived transport vesicles; involved in
retrograde transport between Golgi and ER"
/codon_start=1
/product="coatomer subunit zeta"
/protein_id="NP_015315.1"
/db_xref="GeneID:856097"
/db_xref="SGD:S000005931"
/translation="MSSLSLYTVQAVLILDQQGERIYAKYYQPPHRSDEGHQLLFNSV
KKQKEFEKQLYRKTHKQDSEILIFEDHLVLYKEYIDITIYLVASLEENEIVLQQGFSA
IRGALDLILNSGMDKKNIQENYDMVLLAIDETIDNGVILETDSNTIASRVSKPPTNEP
QMALDLDKGFLGAWGFAKSKFQERLQQGL"
gene complement(<535820..>538936)
/gene="RQC2"
/locus_tag="YPL009C"
/gene_synonym="TAE2"
/db_xref="GeneID:856098"
mRNA complement(<535820..>538936)
/gene="RQC2"
/locus_tag="YPL009C"
/gene_synonym="TAE2"
/product="Rqc2p"
/transcript_id="NM_001183823.1"
/db_xref="GeneID:856098"
CDS complement(535820..538936)
/gene="RQC2"
/locus_tag="YPL009C"
/gene_synonym="TAE2"
/experiment="EXISTENCE:direct assay:GO:0000049 tRNA
binding [PMID:25554787]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:23222640|PMID:26777405]"
/experiment="EXISTENCE:direct assay:GO:0022626 cytosolic
ribosome [PMID:31133701|PMID:25554787]"
/experiment="EXISTENCE:direct assay:GO:0043023 ribosomal
large subunit binding [PMID:25554787]"
/experiment="EXISTENCE:direct assay:GO:0072344 rescue of
stalled cytosolic ribosome [PMID:25554787]"
/experiment="EXISTENCE:direct assay:GO:0140708 CAT tailing
[PMID:31133701]"
/experiment="EXISTENCE:direct assay:GO:1904678
alpha-aminoacyl-tRNA binding [PMID:31133701]"
/experiment="EXISTENCE:direct assay:GO:1990112 RQC complex
[PMID:23178123]"
/experiment="EXISTENCE:genetic interaction:GO:1990116
ribosome-associated ubiquitin-dependent protein catabolic
process [PMID:23479637]"
/experiment="EXISTENCE:mutant phenotype:GO:0043043 peptide
biosynthetic process [PMID:25554787]"
/experiment="EXISTENCE:mutant phenotype:GO:0072344 rescue
of stalled cytosolic ribosome [PMID:26943317]"
/experiment="EXISTENCE:mutant phenotype:GO:1990116
ribosome-associated ubiquitin-dependent protein catabolic
process [PMID:23479637]"
/experiment="EXISTENCE:physical interaction:GO:1990112 RQC
complex [PMID:23479637]"
/note="Component of the Ribosome Quality Control (RQC)
complex; RQC is a ribosome-bound complex required for
degradation of polypeptides arising from stalled
translation; recruits ala- and thr-charged tRNA to the A
site and directs CAT (carboxyl-terminal alanine and
threonine) tailing, the noncanonical elongation of nascent
chains independent of mRNA or 40S subunits, promoting
LTN1-mediated ubiquitination and peptide release from
stalled ribosomes; monitors translation stress and signals
this to Hsf1p"
/codon_start=1
/product="Rqc2p"
/protein_id="NP_015316.1"
/db_xref="GeneID:856098"
/db_xref="SGD:S000005930"
/translation="MKQRISALDLLLLARELKQDLEGYRLSNIYNIADSSKQFLLKFN
KPDSKLNVVVDCGLRIYLTEFSRPIPPTPSGFVVKLRKHLKAKRLTALKQVDQDRILV
LQFADGHFYLVLEFFSAGNVILLDENRRIMALQRVVLEHENKVGQIYEMFDESLFTTN
NESADESIEKNRKAEYTSELVNEWIKAVQAKYESDITVIKQLNIQGKEGAKKKKVKVP
SIHKLLLSKVPHLSSDLLSKNLKVFNIDPSESCLNLLEETDSLAELLNSTQLEYNQLL
TTTDRKGYILAKRNENYISEKDTADLEFIYDTFHPFKPYINGGDTDSSCIIEVEGPYN
RTLDKFFSTIESSKYALRIQNQESQAQKKIDDARAENDRKIQALLDVQELNERKGHLI
IENAPLIEEVKLAVQGLIDQQMDWNTIEKLIKSEQKKGNRIAQLLNLPLNLKQNKISV
KLDLSSKELNTSSDEDNESEGNTTDSSSDSDSEDMESSKERSTKSMKRKSNEKINVTI
DLGLSAYANATEYFNIKKTSAQKQKKVEKNVGKAMKNIEVKIDQQLKKKLKDSHSVLK
KIRTPYFFEKYSWFISSEGFLVMMGKSPAETDQIYSKYIEDDDIYMSNSFNSHVWIKN
PEKTEVPPNTLMQAGILCMSSSEAWSKKISSSPWWCFAKNVSKFDGSDNSILPEGAFR
LKNENDQNHLPPAQLVMGFGFLWKVKTSGNEDNGDDDEEEEEEEEEEEEEEEEEEEEE
EEEKEEEEKEEEQQQDEDDSNEVNGLEKGGDSNDSTKNNSFEHDNLEKDIEKHCTISS
DTDSDSGNAKAKNDNSSTQRILDEPGVPISLIENINSNVRGKRGKLKKIQKKYADQDE
TERLLRLEALGTLKGIEKQQQRKKEEIMKREVREDRKNKREKQRRLQALKFTKKEKAR
VNYDKHKSELKPSLDKGDVVDDIIPVFAPWPALLKYKYKVKIQPGSAKKTKTLTEILH
YFKSRPLDGSSTDNEMDWPQEHEMIKGLKEQDLVLLLCVDKLKVTIAGQKSTKNGGNS
SKKGKKKR"
gene <539385..>541970
/gene="CHL1"
/locus_tag="YPL008W"
/gene_synonym="CTF1; LPA9; MCM12"
/db_xref="GeneID:856099"
mRNA <539385..>541970
/gene="CHL1"
/locus_tag="YPL008W"
/gene_synonym="CTF1; LPA9; MCM12"
/product="DNA helicase"
/transcript_id="NM_001183822.1"
/db_xref="GeneID:856099"
CDS 539385..541970
/gene="CHL1"
/locus_tag="YPL008W"
/gene_synonym="CTF1; LPA9; MCM12"
/EC_number="5.6.2.3"
/experiment="EXISTENCE:genetic interaction:GO:0007064
mitotic sister chromatid cohesion
[PMID:17483413|PMID:15020404|PMID:14742714]"
/experiment="EXISTENCE:genetic interaction:GO:0036297
interstrand cross-link repair [PMID:22912599]"
/experiment="EXISTENCE:mutant phenotype:GO:0000785
chromatin [PMID:24086532]"
/experiment="EXISTENCE:mutant phenotype:GO:0003678 DNA
helicase activity [PMID:10931920]"
/experiment="EXISTENCE:mutant phenotype:GO:0007064 mitotic
sister chromatid cohesion [PMID:15020404|PMID:14742714]"
/experiment="EXISTENCE:mutant phenotype:GO:0031571 mitotic
G1 DNA damage checkpoint signaling [PMID:34493312]"
/experiment="EXISTENCE:mutant phenotype:GO:0034085
establishment of sister chromatid cohesion
[PMID:23334284]"
/experiment="EXISTENCE:physical interaction:GO:0007064
mitotic sister chromatid cohesion [PMID:15020404]"
/note="Probable DNA helicase; involved in sister-chromatid
cohesion and genome integrity and interstrand cross-link
repair; interacts with ECO1 and CTF18; mutants are
defective in silencing, rDNA recombination, aging and the
heat shock response; FANCJ-like helicase family member;
mutations in the human homolog, DDX11/ChLR1, cause Warsaw
breakage syndrome"
/codon_start=1
/product="DNA helicase"
/protein_id="NP_015317.1"
/db_xref="GeneID:856099"
/db_xref="SGD:S000005929"
/translation="MDKKEYSETFYHPYKPYDIQVQLMETVYRVLSEGKKIAILESPT
GTGKTLSLICATMTWLRMNKADIFTRMETNIKTNEDDSENLSDDEPDWVIDTYRKSVL
QEKVDLLNDYEKHLNEINTTSCKQLKTMCDLDKEHGRYKSVDPLRKKRKGARHLDVSL
EEQDFIPRPYESDSENNDTSKSTRGGRISDKDYKLSELNSQIITLLDKIDGKVSRDPN
NGDRFDVTNQNPVKIYYASRTYSQLGQFTSQLRLPSFPSSFRDKVPDEKVKYLPLASK
KQLCINPKVMKWKTLEAINDACADLRHSKEGCIFYQNTNEWRHCPDTLALRDMIFSEI
QDIEDLVPLGKSLGICPYYASREALPIAEVVTLPYQYLLSESTRSSLQINLENSIVII
DEAHNLIETINSIYSSQISLEDLKNCHKGIVTYFNKFKSRLNPGNRVNLLKLNSLLMT
LIQFIVKNFKKIGQEIDPNDMFTGSNIDTLNIHKLLRYIKVSKIAYKIDTYNQALKEE
ESSKNENPIKETHKKSVSSQPLLFKVSQFLYCLTNLTSEGQFFFEKNYSIKYMLLEPS
KPFESILNQAKCVVLAGGTMEPMSEFLSNLLPEVPSEDITTLSCNHVIPKENLQTYIT
NQPELEFTFEKRMSPSLVNNHLFQFFVDLSKAVPKKGGIVAFFPSYQYLAHVIQCWKQ
NDRFATLNNVRKIFYEAKDGDDILSGYSDSVAEGRGSLLLAIVGGKLSEGINFQDDLC
RAVVMVGLPFPNIFSGELIVKRKHLAAKIMKSGGTEEEASRATKEFMENICMKAVNQS
VGRAIRHANDYANIYLLDVRYNRPNFRKKLSRWVQDSINSEHTTHQVISSTRKFFSMR
SLNSR"
gene complement(<542082..>543848)
/gene="TFC8"
/locus_tag="YPL007C"
/db_xref="GeneID:856100"
mRNA complement(<542082..>543848)
/gene="TFC8"
/locus_tag="YPL007C"
/product="transcription factor TFIIIC subunit TFC8"
/transcript_id="NM_001183821.1"
/db_xref="GeneID:856100"
CDS complement(542082..543848)
/gene="TFC8"
/locus_tag="YPL007C"
/experiment="EXISTENCE:direct assay:GO:0000127
transcription factor TFIIIC complex
[PMID:2002052|PMID:10567530|PMID:1474578]"
/experiment="EXISTENCE:direct assay:GO:0001002 RNA
polymerase III type 1 promoter sequence-specific DNA
binding [PMID:2404611|PMID:2649882]"
/experiment="EXISTENCE:direct assay:GO:0001003 RNA
polymerase III type 2 promoter sequence-specific DNA
binding [PMID:2404611]"
/experiment="EXISTENCE:direct assay:GO:0006383
transcription by RNA polymerase III
[PMID:3633923|PMID:2183033|PMID:8464480]"
/experiment="EXISTENCE:direct assay:GO:0008301 DNA
binding, bending [PMID:2002052]"
/experiment="EXISTENCE:direct assay:GO:0042791 5S class
rRNA transcription by RNA polymerase III [PMID:2183033]"
/experiment="EXISTENCE:genetic interaction:GO:0006383
transcription by RNA polymerase III [PMID:10567530]"
/experiment="EXISTENCE:mutant phenotype:GO:0006383
transcription by RNA polymerase III [PMID:10567530]"
/note="Subunit of RNA polymerase III transcription
initiation factor complex; one of six subunits of RNA
polymerase III transcription initiation factor complex
(TFIIIC); part of TFIIIC TauB domain that binds BoxB
promoter sites of tRNA and other genes; linker between
TauB and TauA domains; human homolog is TFIIIC-90"
/codon_start=1
/product="transcription factor TFIIIC subunit TFC8"
/protein_id="NP_015318.1"
/db_xref="GeneID:856100"
/db_xref="SGD:S000005928"
/translation="MKLLKDLLVDRKEFEDWKNNLTWARDGTLYLTTFPDISIGQPKY
AKDINCNSKNLFHVKEFPLEFENKLDFELAQQNGLLNSQPVCYPRVCKPSPIDDWMAV
LSNNGNVSVFKDNKMLTNLDSKGNLSSRTYHCFEWNPIESSIVVGNEDGELQFFSIRK
NSENTPEFYFESSIRLSDAGSKDWVTHIVWYEDVLVAALSNNSVFSMTVSASSHQPVS
RMIQNASRRKITDLKIVDYKVVLTCPGYVHKIDLKNYSISSLKTGSLENFHIIPLNHE
KESTILLMSNKTSYKVLLEDELHVTADNIIAPYLEKKFKKWSTIWNEFNNYETTLVIH
GISLSPDGYSIAIVYDMERVAFKYKIASEQSFNIMFAPLYHTWTISERAVGLAWYQTY
QIYNQSLPKLPENFSMNKKLLNGNYPISLDFQSYLNALMKSEEMRIIMFLNMTIDKPS
ILSFLEALYEYAINKKSELTNSFDLACVLSIAAILKREAPIYNGTLLMKNSFLEETFN
LESFTADPETVTSTTNNTWKRCGVTLLPILTTHVKICPVSKQRVIDIKRDDLNDYGWF
TRGLLERFNEISVYCGTTLEVM"
gene <544631..>548143
/gene="NCR1"
/locus_tag="YPL006W"
/db_xref="GeneID:856101"
mRNA <544631..>548143
/gene="NCR1"
/locus_tag="YPL006W"
/product="sphingolipid transporter"
/transcript_id="NM_001183820.1"
/db_xref="GeneID:856101"
CDS 544631..548143
/gene="NCR1"
/locus_tag="YPL006W"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:16138904|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0015918 sterol
transport [PMID:31543266]"
/experiment="EXISTENCE:direct assay:GO:0032934 sterol
binding [PMID:31543266]"
/experiment="EXISTENCE:mutant phenotype:GO:0006665
sphingolipid metabolic process [PMID:14970192]"
/note="Vacuolar membrane protein involved in sterol
trafficking with Npc2p; conformational changes drive
sterol translocation across the glycocalyx and into the
vacuolar membrane; involved in sphingolipid metabolism;
cells lacking Ncr1p exhibit high levels of long chain
bases (LCBs), similar to accumulation of lipids observed
in patients with Neimann-Pick C disease due to
loss-of-function mutations in human NPC1; transits through
the vacuolar protein sorting pathway via the AP-3 pathway"
/codon_start=1
/product="sphingolipid transporter"
/protein_id="NP_015319.1"
/db_xref="GeneID:856101"
/db_xref="SGD:S000005927"
/translation="MNVLWIIALVGQLMRLVQGTATCAMYGNCGKKSVFGNELPCPVP
RSFEPPVLSDETSKLLVEVCGEEWKEVRYACCTKDQVVALRDNLQKAQPLISSCPACL
KNFNNLFCHFTCAADQGRFVNITKVEKSKEDKDIVAELDVFMNSSWASEFYDSCKNIK
FSATNGYAMDLIGGGAKNYSQFLKFLGDAKPMLGGSPFQINYKYDLANEEKEWQEFND
EVYACDDAQYKCACSDCQESCPHLKPLKDGVCKVGPLPCFSLSVLIFYTICALFAFMW
YYLCKRKKNGAMIVDDDIVPESGSLDESETNVFESFNNETNFFNGKLANLFTKVGQFS
VENPYKILITTVFSIFVFSFIIFQYATLETDPINLWVSKNSEKFKEKEYFDDNFGPFY
RTEQIFVVNETGPVLSYETLHWWFDVENFITEELQSSENIGYQDLCFRPTEDSTCVIE
SFTQYFQGALPNKDSWKRELQECGKFPVNCLPTFQQPLKTNLLFSDDDILNAHAFVVT
LLLTNHTQSANRWEERLEEYLLDLKVPEGLRISFNTEISLEKELNNNNDISTVAISYL
MMFLYATWALRRKDGKTRLLLGISGLLIVLASIVCAAGFLTLFGLKSTLIIAEVIPFL
ILAIGIDNIFLITHEYDRNCEQKPEYSIDQKIISAIGRMSPSILMSLLCQTGCFLIAA
FVTMPAVHNFAIYSTVSVIFNGVLQLTAYVSILSLYEKRSNYKQITGNEETKESFLKT
FYFKMLTQKRLIIIIFSAWFFTSLVFLPEIQFGLDQTLAVPQDSYLVDYFKDVYSFLN
VGPPVYMVVKNLDLTKRQNQQKICGKFTTCERDSLANVLEQERHRSTITEPLANWLDD
YFMFLNPQNDQCCRLKKGTDEVCPPSFPSRRCETCFQQGSWNYNMSGFPEGKDFMEYL
SIWINAPSDPCPLGGRAPYSTALVYNETSVSASVFRTAHHPLRSQKDFIQAYSDGVRI
SSSFPELDMFAYSPFYIFFVQYQTLGPLTLKLIGSAIILIFFISSVFLQNIRSSFLLA
LVVTMIIVDIGALMALLGISLNAVSLVNLIICVGLGVEFCVHIVRSFTVVPSETKKDA
NSRVLYSLNTIGESVIKGITLTKFIGVCVLAFAQSKIFDVFYFRMWFTLIIVAALHAL
LFLPALLSLFGGESYRDDSIEAED"
gene <548486..>550306
/gene="AEP3"
/locus_tag="YPL005W"
/db_xref="GeneID:856102"
mRNA <548486..>550306
/gene="AEP3"
/locus_tag="YPL005W"
/product="Aep3p"
/transcript_id="NM_001183819.1"
/db_xref="GeneID:856102"
CDS 548486..550306
/gene="AEP3"
/locus_tag="YPL005W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0070124
mitochondrial translational initiation [PMID:19843529]"
/experiment="EXISTENCE:mutant phenotype:GO:0016071 mRNA
metabolic process [PMID:14742425]"
/note="Peripheral mitochondrial inner membrane protein;
may facilitate use of unformylated tRNA-Met in
mitochondrial translation initiation; stabilizes the
bicistronic AAP1-ATP6 mRNA"
/codon_start=1
/product="Aep3p"
/protein_id="NP_015320.1"
/db_xref="GeneID:856102"
/db_xref="SGD:S000005926"
/translation="MNTLRCLTQALSKSGREAPKLYQKVIFPGLFREGIPIANVKKVD
EKIIDSPTSTSVNGEAKKIVRHGVKYEREQVKEYLSSLPTLTLSRKQIRDDYDEERAK
RMYMFSKQTNSSNKFQKLLTAKSQEFTRELLTLLIDCTSNEKNSGPERFTRKFLKFSN
DEIPPLPDFSKNPQLFENYIGILSHTKFNFRSSSKLNGIVRKMLRHLLHPTNKTTLPL
RSAQVYNDSIYFFSEHFDFASCREIFAQMKAEGTKPNTITFNLLLRNVVKNSHIRKTK
HPDDEVLFYLRSMRNHGVFADVITWTTCYNFLRDEVSRQLYIVQMGEHLGNFNVNFVY
TVLRNGDYRAEDCLKVLAANSLPISRKTFYLCIERLLNEEQLETASKLLDYGFQHLKS
NFKLDSEAMNHFMRVFANKGRSDLAFLCYNTCRKIYKIKPDSQTFEMLFKALVRNGNT
KNFGAVLQYIKDLKVSEGFGLRTSYWRTKADSIFKFGSPNTLSEKSIEKARKLLGNLI
ASEGEFSWKIWKESDSSQKKILRFLGCIPTTLRCTNTAQDHQKPTNLPSNISQKKREY
RNRVKAIATKAALEKRMAYIKDNDVAFKKELVKRRIVGEV"
gene complement(<550632..>551657)
/gene="LSP1"
/locus_tag="YPL004C"
/db_xref="GeneID:856103"
mRNA complement(<550632..>551657)
/gene="LSP1"
/locus_tag="YPL004C"
/product="lipid-binding protein LSP1"
/transcript_id="NM_001183818.1"
/db_xref="GeneID:856103"
CDS complement(550632..551657)
/gene="LSP1"
/locus_tag="YPL004C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:16407407]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836]"
/experiment="EXISTENCE:direct assay:GO:0008289 lipid
binding [PMID:22123866]"
/experiment="EXISTENCE:direct assay:GO:0032126 eisosome
[PMID:16496001]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0006897
endocytosis [PMID:16496001]"
/experiment="EXISTENCE:mutant phenotype:GO:0009408
response to heat [PMID:15016821]"
/experiment="EXISTENCE:mutant phenotype:GO:0061024
membrane organization [PMID:38096056]"
/experiment="EXISTENCE:mutant phenotype:GO:0070941
eisosome assembly [PMID:36601791]"
/note="Stress-responsive eisosome organizer and core
component; eisosomes are large immobile patch structures
at the cell cortex associated with endocytosis;
phosphorylated on Thr233 upon Pkc1p hyperactivation in a
Slt2p MAPK-dependent fashion; null mutants show activation
of Pkc1p/Ypk1p stress resistance pathways; member of the
BAR domain family"
/codon_start=1
/product="lipid-binding protein LSP1"
/protein_id="NP_015321.1"
/db_xref="GeneID:856103"
/db_xref="SGD:S000005925"
/translation="MHRTYSLRNQRAPTAAELQAPPPPPSSTKSKFFGKASIASSFRK
NAAGNFGPELARKLSQLVKTEKGVLRAMEVVASERREAAKQLSLWGADNDDDVSDVTD
KLGVLIYELGELQDQFIDKYDQYRVTLKSIRNIEASVQPSRDRKEKITDEIAHLKYKD
PQSTKIPVLEQELVRAEAESLVAEAQLSNITREKLKAAYSYMFDSLRELSEKFALIAG
YGKALLELLDDSPVTPGEARPAYDGYEASRQIIMDAESALESWTLDMAAVKPTLSFHQ
TVDDVYEDEDGEEEEEPEIQNGDIPGQVVEEEEVEWTTEVPVDDEAHEADHHVSQNGH
TSGSENI"
gene <552020..>553408
/gene="ULA1"
/locus_tag="YPL003W"
/gene_synonym="ENR2"
/db_xref="GeneID:856104"
mRNA <552020..>553408
/gene="ULA1"
/locus_tag="YPL003W"
/gene_synonym="ENR2"
/product="Ula1p"
/transcript_id="NM_001183817.1"
/db_xref="GeneID:856104"
CDS 552020..553408
/gene="ULA1"
/locus_tag="YPL003W"
/gene_synonym="ENR2"
/experiment="EXISTENCE:direct assay:GO:0019781 NEDD8
activating enzyme activity [PMID:9545234]"
/experiment="EXISTENCE:direct assay:GO:0045116 protein
neddylation [PMID:9545234]"
/experiment="EXISTENCE:mutant phenotype:GO:0019781 NEDD8
activating enzyme activity [PMID:9531531]"
/experiment="EXISTENCE:mutant phenotype:GO:0045116 protein
neddylation [PMID:9545234]"
/note="Protein that activates Rub1p (NEDD8) before
neddylation; acts together with Uba3p; may play a role in
protein degradation"
/codon_start=1
/product="Ula1p"
/protein_id="NP_015322.1"
/db_xref="GeneID:856104"
/db_xref="SGD:S000005924"
/translation="MERYDRQLRLWGALGQDSLNRSRVCVVGPATPLLQEVFKNLVLA
GISSLTWLKVECAVQSGSLFLAELKKDLEPLASKQLEYEENDLRKTLQQPQYDWTRFS
VVILTCIGEQTAMLDLNEIRRQRGTKFPPVLNTFVSGFYGYIYLVLSETHFVLQAHPD
SKKYDLRLQNPWPELINYVDTFDLSKMDTATFSGIPYTVLLMKCIAKLERDGNNGRIT
IDQMKKVLDQICLPLGNDVIYEPNYVEAKRYAYLACSQNDCCKELEDLLRNLEISDYG
NDWHDTYNYEILTLLLTLKNIAKENGELSFQPLTGTLPDMESTTENYIRLKKLYEVKA
KLDKSRVEESLARSKKIVSQDVLETFCSHYGEVRKILPPKSDLLGIFSTSNALLDALV
MVQFWEQPAVTAEDKDEFIGLRVDDNYSVMAFFGGAVVQEAIKLITHHYVPIDNLFLY
NGINNSSATYKI"
gene complement(<553627..>554328)
/gene="SNF8"
/locus_tag="YPL002C"
/gene_synonym="VPL14; VPS22"
/db_xref="GeneID:856105"
mRNA complement(<553627..>554328)
/gene="SNF8"
/locus_tag="YPL002C"
/gene_synonym="VPL14; VPS22"
/product="ESCRT-II subunit protein SNF8"
/transcript_id="NM_001183816.1"
/db_xref="GeneID:856105"
CDS complement(553627..554328)
/gene="SNF8"
/locus_tag="YPL002C"
/gene_synonym="VPL14; VPS22"
/experiment="EXISTENCE:direct assay:GO:0000814 ESCRT II
complex [PMID:12194858]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:7785322]"
/experiment="EXISTENCE:mutant phenotype:GO:0006623 protein
targeting to vacuole [PMID:12194858]"
/experiment="EXISTENCE:mutant phenotype:GO:1904669 ATP
export [PMID:26585826]"
/note="Component of the ESCRT-II complex; ESCRT-II is
involved in ubiquitin-dependent sorting of proteins into
the endosome; appears to be functionally related to SNF7;
involved in glucose derepression"
/codon_start=1
/product="ESCRT-II subunit protein SNF8"
/protein_id="NP_015323.1"
/db_xref="GeneID:856105"
/db_xref="SGD:S000005923"
/translation="MKQFGLAAFDELKDGKYNDVNKTILEKQSVELRDQLMVFQERLV
EFAKKHNSELQASPEFRSKFMHMCSSIGIDPLSLFDRDKHLFTVNDFYYEVCLKVIEI
CRQTKDMNGGVISFQELEKVHFRKLNVGLDDLEKSIDMLKSLECFEIFQIRGKKFLRS
VPNELTSDQTKILEICSILGYSSISLLKANLGWEAVRSKSALDEMVANGLLWIDYQGG
AEALYWDPSWITRQL"
gene <554605..>555729
/gene="HAT1"
/locus_tag="YPL001W"
/gene_synonym="KAT1"
/db_xref="GeneID:856106"
mRNA <554605..>555729
/gene="HAT1"
/locus_tag="YPL001W"
/gene_synonym="KAT1"
/product="histone acetyltransferase catalytic subunit
HAT1"
/transcript_id="NM_001183815.1"
/db_xref="GeneID:856106"
CDS 554605..555729
/gene="HAT1"
/locus_tag="YPL001W"
/gene_synonym="KAT1"
/EC_number="2.3.1.48"
/experiment="EXISTENCE:direct assay:GO:0000123 histone
acetyltransferase complex [PMID:9575221|PMID:8858151]"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:16612003]"
/experiment="EXISTENCE:direct assay:GO:0004402 histone
acetyltransferase activity [PMID:7559580|PMID:8858151]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14761951|PMID:9575221]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:8858151|PMID:9575221]"
/experiment="EXISTENCE:direct assay:GO:0006302
double-strand break repair [PMID:16612003]"
/experiment="EXISTENCE:genetic interaction:GO:0031509
subtelomeric heterochromatin formation
[PMID:15099519|PMID:10982821]"
/experiment="EXISTENCE:mutant phenotype:GO:0004402 histone
acetyltransferase activity
[PMID:7559580|PMID:8858151|PMID:9575221]"
/experiment="EXISTENCE:physical interaction:GO:0000123
histone acetyltransferase complex [PMID:8858151]"
/note="Catalytic subunit of the Hat1p-Hat2p histone
acetyltransferase complex; uses the cofactor acetyl
coenzyme A to acetylate free nuclear and cytoplasmic
histone H4; involved in telomeric silencing and DNA
double-strand break repair"
/codon_start=1
/product="histone acetyltransferase catalytic subunit
HAT1"
/protein_id="NP_015324.1"
/db_xref="GeneID:856106"
/db_xref="SGD:S000005922"
/translation="MSANDFKPETWTSSANEALRVSIVGENAVQFSPLFTYPIYGDSE
KIYGYKDLIIHLAFDSVTFKPYVNVKYSAKLGDDNIVDVEKKLLSFLPKDDVIVRDEA
KWVDCFAEERKTHNLSDVFEKVSEYSLNGEEFVVYKSSLVDDFARRMHRRVQIFSLLF
IEAANYIDETDPSWQIYWLLNKKTKELIGFVTTYKYWHYLGAKSFDEDIDKKFRAKIS
QFLIFPPYQNKGHGSCLYEAIIQSWLEDKSITEITVEDPNEAFDDLRDRNDIQRLRKL
GYDAVFQKHSDLSDEFLESSRKSLKLEERQFNRLVEMLLLLNNSPSFELKVKNRLYIK
NYDALDQTDPEKAREALQNSFILVKDDYRRIIESINKSQG"
centromere 555957..556073
/note="CEN16; Chromosome XVI centromere"
/db_xref="SGD:S000006477"
centromere 555957..555966
/note="CEN16_CDEI of CEN16"
centromere 555967..556048
/note="CEN16_CDEII of CEN16"
centromere 556049..556073
/note="CEN16_CDEIII of CEN16"
gene <556377..>557837
/gene="CIT3"
/locus_tag="YPR001W"
/db_xref="GeneID:856107"
mRNA <556377..>557837
/gene="CIT3"
/locus_tag="YPR001W"
/product="citrate (Si)-synthase CIT3"
/transcript_id="NM_001184098.1"
/db_xref="GeneID:856107"
CDS 556377..557837
/gene="CIT3"
/locus_tag="YPR001W"
/EC_number="2.3.3.1"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:9140965|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0006099
tricarboxylic acid cycle [PMID:17570335]"
/experiment="EXISTENCE:direct assay:GO:0036440 citrate
synthase activity [PMID:17570335]"
/experiment="EXISTENCE:direct assay:GO:0050440
2-methylcitrate synthase activity [PMID:17570335]"
/experiment="EXISTENCE:genetic interaction:GO:0019629
propionate catabolic process, 2-methylcitrate cycle
[PMID:17570335]"
/experiment="EXISTENCE:mutant phenotype:GO:0036440 citrate
synthase activity [PMID:17570335]"
/experiment="EXISTENCE:mutant phenotype:GO:0050440
2-methylcitrate synthase activity [PMID:17570335]"
/note="Dual specificity mitochondrial citrate and
methylcitrate synthase; catalyzes the condensation of
acetyl-CoA and oxaloacetate to form citrate and that of
propionyl-CoA and oxaloacetate to form 2-methylcitrate"
/codon_start=1
/product="citrate (Si)-synthase CIT3"
/protein_id="NP_015325.1"
/db_xref="GeneID:856107"
/db_xref="SGD:S000006205"
/translation="MVQRLLPGAHICRRSFNSSAIIKSSALTLKEALENVIPKKRDAV
KKLKACYGSTFVGPITISSVLGGMRGNQSMFWQGTSLDPEHGIKFQGLTIEECQNRLP
NTGIDGDNFLPESMLWLLMTGGVPTFQQAASFRKELAIRGRKLPHYTEKVLSSLPKDM
HPMTQLAIGLASMNKGSLFATNYQKGLIGKMEFWKDTLEDSLNLIASLPLLTGRIYSN
ITNEGHPLGQYSEEVDWCTNICSLLGMTNGTNSSNTCNLTSQQSLDFINLMRLYTGIH
VDHEGGNVSAHTTHLVGSALSDPYLSYSSGIMGLAGPLHGLAAQEVVRFLIEMNSNIS
SIAREQEIKDYLWKILNSNRVIPGYGHAVLRKPDPRFTAMLEFAQKRPIEFENDKNVL
LMQKLAEIAPKVLLEHGKSKNPFPNVDSASGILFYHYGIRELLFFTVIFGCSRAMGPL
TQLVWDRILGLPIERPKSLNLEGLEALTKASNVNKL"
gene <558385..>559935
/gene="PDH1"
/locus_tag="YPR002W"
/db_xref="GeneID:856108"
mRNA <558385..>559935
/gene="PDH1"
/locus_tag="YPR002W"
/product="putative 2-methylcitrate dehydratase"
/transcript_id="NM_001184099.1"
/db_xref="GeneID:856108"
CDS 558385..559935
/gene="PDH1"
/locus_tag="YPR002W"
/EC_number="4.2.1.79"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:14576278|PMID:16823961|PMID:14562095|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:16407407]"
/experiment="EXISTENCE:mutant phenotype:GO:0019541
propionate metabolic process [PMID:11179416]"
/note="Putative 2-methylcitrate dehydratase; mitochondrial
protein that participates in respiration; induced by
diauxic shift; homologous to E. coli PrpD, may take part
in the conversion of 2-methylcitrate to
2-methylisocitrate"
/codon_start=1
/product="putative 2-methylcitrate dehydratase"
/protein_id="NP_015326.1"
/db_xref="GeneID:856108"
/db_xref="SGD:S000006206"
/translation="MFLAKNLKNNKIKVCLPKKKFAALSTASIQTNERPNPDKVLKDI
AKYVHETPLKSSLALDTARLCFLDTLGCGLAALKFKQAQNIIKPIVPGTIVPSGTKIL
GTSYVMDPVKGAFAIGTLIRWLDYNDCWLAAEWGHPSDNLGGILAVADHLSRLNKATH
GKNGKQFLVKDVLEAMIKAHEIQGIIALENSFNKVGLDHVVLVKVATAGVVSKMLGLS
QEQTIEALSQAFVDGQSLRTYRHAPNTGSRKSWAAGDAVSRAVNLAYLVKNANVGTIP
SVLTARTWGFYDVLFKGKPFSFQQRSKYDSYVMENVLFKISFPAEFHAQTAVEAAVKA
YRILAKQGKTFKDIKSIRIRTQEAAMRIIDKSGPLYNYADRDHCIQYMIAVPLITGNL
TATDYSDEVARNPEIDNLRSKMYCIEDTHLTQNYHDPDKRSIGNALLIELNDGTQLDE
IFVEYPVGHKFRREEGIPLLMNKFQRHLREHFVESPDKVDLIMKVSSKTNFLNMQIDK
YMDLFTEG"
gene complement(560198..560289)
/locus_tag="YNCP0008C"
/db_xref="GeneID:856109"
tRNA complement(join(560198..560233,560253..560289))
/locus_tag="YNCP0008C"
/product="tRNA-Phe"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:10024171]"
/note="Phenylalanine tRNA (tRNA-Phe), predicted by
tRNAscan-SE analysis"
/db_xref="GeneID:856109"
/db_xref="SGD:S000006570"
repeat_region 560481..560812
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007210"
gene complement(<561504..>563768)
/locus_tag="YPR003C"
/db_xref="GeneID:856111"
mRNA complement(<561504..>563768)
/locus_tag="YPR003C"
/product="uncharacterized protein"
/transcript_id="NM_001184100.1"
/db_xref="GeneID:856111"
CDS complement(561504..563768)
/locus_tag="YPR003C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095]"
/note="Putative sulfate permease; physically interacts
with Hsp82p; green fluorescent protein (GFP)-fusion
protein localizes to the ER; YPR003C is not an essential
gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015328.1"
/db_xref="GeneID:856111"
/db_xref="SGD:S000006207"
/translation="MTSNNSLLGRGRMSYSSTAPPRFKRSVDQRDTFSDNFDYDKDSS
NRGRTYIAASNSTTGVPPPNNSRSGCTNNTNNTNNTSNTSNTNNNDSVDENTVFETLP
YYLPCFSWLPEYTFNKLWGDVIAGISVASFQIPLALSYTTSIAHVPPLCGLYSLAISP
FVYGILGSVPQMIVGPESAISLVVGQAVESITLHKENVSLIDISTVITFVSGTILLFS
GISRFGFLGNVLSKALLRGFISSVGLVMIINSLISELKLDKFLVSLPQHYHTPFEKIL
FLIDYAPAQYHIPTAIFSGCCLIVLFLTRLLKRKLMKYHKSAIFFPDILLVVIVTILI
SMKFNLKHRYGISIIGDFSMDNFDELKNPLTRPRRKLIPDLFSASLIVAMLGFFESTT
ASKSLGTTYNLTVSSNRELVALGFMNIVISLFGALPAFGGYGRSKINALSGAQSVMSG
VFMGVITLITMNLLLQFVHYIPNCVLSVITTIIGISLLEEVPGDIKFHLRCGGFSELF
VFAVTFCTTIFYSIEAGICIGCVYSIINIIKHSAKSRIQILARVAGTSNFTNLDDYMM
NMKRNSLDVEGTEEIEGCMIVRIPEPLTFTNSEDLKQRLDRIERYGSSKIHPGRKSLR
SKDSIKYVIFDLGGMTSIDSSAAQVLEEIITSYKRRNVFIYLVNVSINDKVRRRLFKA
GVAASVERAQANNNENNTSNTFSDAGETYSPYFDSIDAALYEIEKMKIKGNNVPNNDS
ESFMSNTLFNSSLV"
rep_origin 563852..563967
/note="ARS1622; Autonomously Replicating Sequence"
/db_xref="SGD:S000118418"
gene complement(<564007..>565041)
/gene="AIM45"
/locus_tag="YPR004C"
/db_xref="GeneID:856112"
mRNA complement(<564007..>565041)
/gene="AIM45"
/locus_tag="YPR004C"
/product="Aim45p"
/transcript_id="NM_001184101.1"
/db_xref="GeneID:856112"
CDS complement(564007..565041)
/gene="AIM45"
/locus_tag="YPR004C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:17054397|PMID:11502169|PMID:14562095|PMID:14576278|P
MID:24769239|PMID:16823961]"
/note="Putative ortholog of mammalian ETF-alpha; interacts
with frataxin, Yfh1p; null mutant displays elevated
frequency of mitochondrial genome loss; may have a role in
oxidative stress response; ETF-alpha is an electron
transfer flavoprotein complex subunit"
/codon_start=1
/product="Aim45p"
/protein_id="NP_015329.1"
/db_xref="GeneID:856112"
/db_xref="SGD:S000006208"
/translation="MFKSLAAVLPRASKAKFLQKNYASTLAFIESSKDGSVSRSSLSL
LAAAQKLSNPITAVITGSKAEKTAEALKSSYSCSNLEKLVIFEDSKLDTCLPEQLTPL
LVKLLKGGDYSHFVVSNSSVGKSVLPRVGALLDVQPVCEVTVIKDPKTFIRPIYAGNI
ISTIECQAEKKLLIIRASAFPPIAEGSMDSVTIEKRTDIPPCDLNVTWVKTILTKSER
PELTSAQNVVTGGRALKDKETFEKLLSPLADVLHAAIGATRASVDNGLCDNSLQIGQT
GKVVAPNLYIAIGVSGAVQHLAGMKDSKVIVAINNDPDAPIFNVADYGLQGDLYKIVP
ELTEKLGKYK"
rep_origin 565052..565294
/note="ARS1634; Autonomously Replicating Sequence"
/db_xref="SGD:S000119040"
gene complement(<565787..>566671)
/gene="HAL1"
/locus_tag="YPR005C"
/db_xref="GeneID:856113"
mRNA complement(<565787..>566671)
/gene="HAL1"
/locus_tag="YPR005C"
/product="Hal1p"
/transcript_id="NM_001184102.1"
/db_xref="GeneID:856113"
CDS complement(565787..566671)
/gene="HAL1"
/locus_tag="YPR005C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:1505513]"
/experiment="EXISTENCE:mutant phenotype:GO:0009651
response to salt stress [PMID:1505513]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:1505513]"
/note="Cytoplasmic protein involved in halotolerance;
decreases intracellular Na+ (via Ena1p) and increases
intracellular K+ by decreasing efflux; expression
repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl,
KCl, and sorbitol through Gcn4p"
/codon_start=1
/product="Hal1p"
/protein_id="NP_015330.1"
/db_xref="GeneID:856113"
/db_xref="SGD:S000006209"
/translation="MHFKDLGLHDYTLKNLMYENNCCKFYDAVDENNISYVLKFVPSD
VTSEGDTFPFVDRFQVKEGVFLVYSSNDFGKEGTDYFTYTGSGGNEVHISGTSSEAGI
KPQFIETCHPKHLKRGTKEQEDINSSTSKKSAVINNFSGEKTPNPRPQSSNISERETY
VGILNVKCKNKNSSKIRSEKLVSSVIETKHTPGLASILSKEGTTYPNNADGKHISIVN
PSSKIYHSSHKQIVKTPIPKSGLSPIERCPFNGQNIKCYSPRPLDHESPQRDFNNNFQ
LRILKSSVLQRRQSTQNS"
gene complement(<567269..>568996)
/gene="ICL2"
/locus_tag="YPR006C"
/db_xref="GeneID:856114"
mRNA complement(<567269..>568996)
/gene="ICL2"
/locus_tag="YPR006C"
/product="methylisocitrate lyase ICL2"
/transcript_id="NM_001184103.1"
/db_xref="GeneID:856114"
CDS complement(567269..568996)
/gene="ICL2"
/locus_tag="YPR006C"
/EC_number="4.1.3.30"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:11092862]"
/experiment="EXISTENCE:direct assay:GO:0019629 propionate
catabolic process, 2-methylcitrate cycle [PMID:11092862]"
/experiment="EXISTENCE:direct assay:GO:0046421
methylisocitrate lyase activity [PMID:11092862]"
/note="2-methylisocitrate lyase of the mitochondrial
matrix; functions in the methylcitrate cycle to catalyze
the conversion of 2-methylisocitrate to succinate and
pyruvate; ICL2 transcription is repressed by glucose and
induced by ethanol"
/codon_start=1
/product="methylisocitrate lyase ICL2"
/protein_id="NP_015331.1"
/db_xref="GeneID:856114"
/db_xref="SGD:S000006210"
/translation="MITMINNKTFNRKTTGTLKKLVLSSDKSLRRSFNGASSTKDFVF
SESSKVEEWWESARFKNISRPYSATDVVKHRGSLPANTSIYPSSYQARKLFNLLEENF
KNGTPLHTLGVIDPVQMSQLARCRNIKVAYISGWACSSTLVGSTNEVSPDFGDYPYDT
VPNQVERIFKAQQLHDRKAFLEASIKGSTPVDYLKPIIADADMGHGGPTTVMKVAKLF
AEKGAAGIHLEDQMVGGKRCGHLSGAVLVPTATHLMRLISTRFQWDIMGTENLVIART
DSCNGKLLSSSSDPRDHEFIRGIIRDNVVPWSEKLIEMEDKKIPNSAIADMEKEWYHE
NELFTFEEALEKQFTASEFESYKEKKEDLMVNKLGRAYLSLREMKLLAQEVTPLKKII
FDWDAPRTKEGYYMFNGCIEAAIRRSLVFAPYSDMIWLETKTPDLEQARSFSRKIHKQ
LPATKLVYNLSPSFNWSAHGFDDKALKSFVWDLAKEGFTLQLVSLAGLHSDGVSFWEL
ANSFQSDGMKAYVEKVQKREKETNCDIMTHQLWSGAEYVDSLMKVVQNGASSQTLSTS
GESFTETQF"
gene complement(<569336..>571378)
/gene="REC8"
/locus_tag="YPR007C"
/gene_synonym="SPO69"
/db_xref="GeneID:856115"
mRNA complement(<569336..>571378)
/gene="REC8"
/locus_tag="YPR007C"
/gene_synonym="SPO69"
/product="Rec8p"
/transcript_id="NM_001184104.1"
/db_xref="GeneID:856115"
CDS complement(569336..571378)
/gene="REC8"
/locus_tag="YPR007C"
/gene_synonym="SPO69"
/experiment="EXISTENCE:direct assay:GO:0000775 chromosome,
centromeric region [PMID:23246437]"
/experiment="EXISTENCE:direct assay:GO:0000779 condensed
chromosome, centromeric region
[PMID:16357219|PMID:10412984]"
/experiment="EXISTENCE:direct assay:GO:0000794 condensed
nuclear chromosome [PMID:16357219]"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:23246437]"
/experiment="EXISTENCE:genetic interaction:GO:0071459
protein localization to chromosome, centromeric region
[PMID:16357219]"
/experiment="EXISTENCE:mutant phenotype:GO:0007130
synaptonemal complex assembly [PMID:10412984]"
/experiment="EXISTENCE:mutant phenotype:GO:0007131
reciprocal meiotic recombination [PMID:10412984]"
/experiment="EXISTENCE:mutant phenotype:GO:0042138 meiotic
DNA double-strand break formation [PMID:31617566]"
/experiment="EXISTENCE:mutant phenotype:GO:0051177 meiotic
sister chromatid cohesion [PMID:10412984]"
/note="Meiosis-specific component of the sister chromatid
cohesion complex; alpha-kleisin family member that
maintains cohesion between homologous chromosomes during
meiosis I until chromosome arm-associated Rec8p is cleaved
by Esp1p; cohesion between sister chromatid centromeres is
maintained until metaphase of meiosis II when it is
cleaved by Esp1; independent of the role in cohesion,
Rec8p promotes allelic collisions and prevents nonspecific
chromosome interactions; homolog of S. pombe Rec8p"
/codon_start=1
/product="Rec8p"
/protein_id="NP_015332.1"
/db_xref="GeneID:856115"
/db_xref="SGD:S000006211"
/translation="MAPLSLNFKDDKKYKGLTTVWLLSALGNSIVKESNNYYSNKSNS
TGNISSSTVKKKDIVNISIPKTCDEIQNFENDFSLRYISNLLYGVTICYNKKTEYVLN
DLNHLLVQLQKNDVYAFKAKNKSTRINGLNSNNSIIGNKNNNYTWEECVFFDDDPLYD
ITKVPALEFLNTTLQDNVSFIEEAKSIRRQDYINELSNSNRFELHGDMTNSDAQSNLG
SNVRNSFPLDEIPVDVDFNLDLDDIVSHQGTPLGSHSSSQKDGNDFKFNYQGDELVLN
FENDNENNSNGGEDTSVENEGPVANLKDYELGLEAQASEEENDLQQKLNTRMQRGHRA
DVGGQFSKVQFDAKTSYPNEVLKFNHGNYSHLMEKNRIRKLTGQNFLTSNISSLVRSC
GEEEFFSTNWLSIFNDFSNIKTSEWDLYPQGFSSVERGRKRAHSLVSTQSSSSTRSHE
YGRKSFRNNKNDNYSSDMENDNLLLNLEQINEDLEDGHYIEENSQGNILDFNLNLPPS
SFGRSHTRNSTRSSGFNEDIVGALRRRVGPSEQNFAEEDDSSNSCFSDGSQQNLQQDK
TNFQDVILDYQTKKFYDYIKERSIVVGRTTRSNPPFKRKMLLVDIIPSRMGEAQTGAN
FDDVERGVSRQIAASAFLSLLNLATKGMVKLNEYPVADAVTKDLKLRREDEIIVYA"
repeat_region complement(571845..572173)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007200"
gene 572269..572339
/locus_tag="YNCP0009W"
/db_xref="GeneID:856116"
tRNA 572269..572339
/locus_tag="YNCP0009W"
/product="tRNA-Gly"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0030371 translation
repressor activity [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043555 regulation
of translation in response to stress [PMID:27609601]"
/note="Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:856116"
/db_xref="SGD:S000006588"
gene <573018..>575102
/gene="HAA1"
/locus_tag="YPR008W"
/db_xref="GeneID:856117"
mRNA <573018..>575102
/gene="HAA1"
/locus_tag="YPR008W"
/product="Haa1p"
/transcript_id="NM_001184105.1"
/db_xref="GeneID:856117"
CDS 573018..575102
/gene="HAA1"
/locus_tag="YPR008W"
/experiment="EXISTENCE:direct assay:GO:0000981 DNA-binding
transcription factor activity, RNA polymerase II-specific
[PMID:21586585]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11504737]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0045944 positive
regulation of transcription by RNA polymerase II
[PMID:11504737]"
/experiment="EXISTENCE:mutant phenotype:GO:0033554
cellular response to stress [PMID:16176797]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:16980434|PMID:28068993|PMID:16176797|PMID:11504737]"
/experiment="EXISTENCE:mutant phenotype:GO:0071468
cellular response to acidic pH
[PMID:16980434|PMID:28068993]"
/note="Transcriptional activator involved in adaptation to
weak acid stress; activates transcription of TPO2, YRO2,
and other genes encoding membrane stress proteins; HAA1
has a paralog, CUP2, that arose from the whole genome
duplication; relocalizes from cytoplasm to nucleus upon
DNA replication stress"
/codon_start=1
/product="Haa1p"
/protein_id="NP_015333.1"
/db_xref="GeneID:856117"
/db_xref="SGD:S000006212"
/translation="MVLINGIKYACERCIRGHRVTTCNHTDQPLMMIKPKGRPSTTCD
YCKQLRKNKNANPEGVCTCGRLEKKKLAQKAKEEARAKAKEKQRKQCTCGTDEVCKYH
AQKRHLRKSPSSSQKKGRSISRSQPMFERVLSSTSLDSNMLSGHGALSDTSSILTSTF
LDSEPGVGKISKDYHHVPSLASISSLQSSQSLDQNFSIPQSPPLSSMSFNFLTGNINE
TNQNHSNHQHSKSGNNWQDSSVSLPAKADSRLNMMDKNNSVGLDLLGHSKRISPISNS
RVGEVSVPLEEYIPSDIDGVGRVTDKSSLVYDWPFDESIERNFSTTATAATGESKFDI
NDNCNRINSKSYSKTNSMNGNGMNNSNNNNINSNGNDKNNNNSSRQEHQGNGLFDMFT
DSSSISTLSRANLLLQEKIGSQENSVKQENYSKNPQLRHQLTSRSRSFIHHPANEYLK
NTFGNSHSNDIGKGVEVLSLTPSFMDIPEKERETERSPSSNYITDRPFTRKPRSSSID
VNHRYPPMAPTTVATSPGALNNAVASNLDDQLSLTSLNSQPSSIANMMMDPSNLAEQS
SIHSVPQSINSPRMPKTGSRQDKNIHTKKEERNPLNNIHDLSQLENVPDEMNQMFSPP
LKSMNRPDAIRENSSSSNFIIQGNSMISTPSGRNDLPDTSPMSSIQTASPPSQLLTDQ
GFADLDNFMSSL"
gene <576552..>577358
/gene="SUT2"
/locus_tag="YPR009W"
/db_xref="GeneID:856118"
mRNA <576552..>577358
/gene="SUT2"
/locus_tag="YPR009W"
/product="Sut2p"
/transcript_id="NM_001184106.1"
/db_xref="GeneID:856118"
CDS 576552..577358
/gene="SUT2"
/locus_tag="YPR009W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:15030478]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:19158363]"
/experiment="EXISTENCE:genetic interaction:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:23872066]"
/experiment="EXISTENCE:genetic interaction:GO:1900237
positive regulation of induction of conjugation with
cellular fusion [PMID:23872066]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:23872066|PMID:26448198]"
/experiment="EXISTENCE:mutant phenotype:GO:0009267
cellular response to starvation [PMID:26448198]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:11248676]"
/experiment="EXISTENCE:mutant phenotype:GO:1900237
positive regulation of induction of conjugation with
cellular fusion [PMID:23872066]"
/experiment="EXISTENCE:mutant phenotype:GO:1900429
negative regulation of filamentous growth of a population
of unicellular organisms [PMID:26448198]"
/experiment="EXISTENCE:mutant phenotype:GO:2000911
positive regulation of sterol import [PMID:11248676]"
/note="Zn2Cys6 family transcription factor; positively
regulates sterol uptake under anaerobic conditions with
SUT1; represses filamentation-inducing genes during
non-starvation conditions; positively regulates mating
along with SUT1 by repressing the expression of genes
(PRR2, NCE102 and RHO5) which function as mating
inhibitors; multicopy suppressor of mutations that cause
low activity of the cAMP/PKA pathway; SUT2 has a paralog,
SUT1, that arose from the whole genome duplication"
/codon_start=1
/product="Sut2p"
/protein_id="NP_015334.1"
/db_xref="GeneID:856118"
/db_xref="SGD:S000006213"
/translation="MKPNNRTCDVITNKDESLPALLLPALNSYTCDDSLLKGQISSNG
RYQPFGFSDCSLLPKRLNIQAGQGSMPVSSIQCADHSYSNWQKESEKTKLPKLGCPTE
YTEYYKTVSSGETTDSAVVSSIATNRLKRKRQRDGPSCDSCRIKKIKCNATIIIFLQD
RNLISSISSNLHYTLSQDDINQFRMKFFRKLPDVMGTYEVIKHLDKIVLFKACTSCSR
RNQKNGKCLFSRGFTKSDMNVFPKINSKLKDKSIFEMTVDDYVAAGFQTL"
gene complement(<577585..>581196)
/gene="RPA135"
/locus_tag="YPR010C"
/gene_synonym="RPA2; RRN2; SRP3"
/db_xref="GeneID:856119"
mRNA complement(<577585..>581196)
/gene="RPA135"
/locus_tag="YPR010C"
/gene_synonym="RPA2; RRN2; SRP3"
/product="DNA-directed RNA polymerase I core subunit
RPA135"
/transcript_id="NM_001184107.1"
/db_xref="GeneID:856119"
CDS complement(577585..581196)
/gene="RPA135"
/locus_tag="YPR010C"
/gene_synonym="RPA2; RRN2; SRP3"
/EC_number="2.7.7.6"
/experiment="EXISTENCE:direct assay:GO:0003899
DNA-directed RNA polymerase activity [PMID:9837969]"
/experiment="EXISTENCE:direct assay:GO:0005736 RNA
polymerase I complex [PMID:9837969|PMID:11486042]"
/experiment="EXISTENCE:direct assay:GO:0006360
transcription by RNA polymerase I [PMID:9837969]"
/experiment="EXISTENCE:genetic interaction:GO:0042790
nucleolar large rRNA transcription by RNA polymerase I
[PMID:2023944]"
/note="RNA polymerase I second largest subunit A135"
/codon_start=1
/product="DNA-directed RNA polymerase I core subunit
RPA135"
/protein_id="NP_015335.1"
/db_xref="GeneID:856119"
/db_xref="SGD:S000006214"
/translation="MSKVIKPPGQARTADFRTLERESRFINPPKDKSAFPLLQEAVQP
HIGSFNALTEGPDGGLLNLGVKDIGEKVIFDGKPLNSEDEISNSGYLGNKLSVSVEQV
SIAKPMSNDGVSSAVERKVYPSESRQRLTSYRGKLLLKLKWSVNNGEENLFEVRDCGG
LPVMLQSNRCHLNKMSPYELVQHKEESDEIGGYFIVNGIEKLIRMLIVQRRNHPMAII
RPSFANRGASYSHYGIQIRSVRPDQTSQTNVLHYLNDGQVTFRFSWRKNEYLVPVVMI
LKALCHTSDREIFDGIIGNDVKDSFLTDRLELLLRGFKKRYPHLQNRTQVLQYLGDKF
RVVFQASPDQSDLEVGQEVLDRIVLVHLGKDGSQDKFRMLLFMIRKLYSLVAGECSPD
NPDATQHQEVLLGGFLYGMILKEKIDEYLQNIIAQVRMDINRGMAINFKDKRYMSRVL
MRVNENIGSKMQYFLSTGNLVSQSGLDLQQVSGYTVVAEKINFYRFISHFRMVHRGSF
FAQLKTTTVRKLLPESWGFLCPVHTPDGSPCGLLNHFAHKCRISTQQSDVSRIPSILY
SLGVAPASHTFAAGPSLCCVQIDGKIIGWVSHEQGKIIADTLRYWKVEGKTPGLPIDL
EIGYVPPSTRGQYPGLYLFGGHSRMLRPVRYLPLDKEDIVGPFEQVYMNIAVTPQEIQ
NNVHTHVEFTPTNILSILANLTPFSDFNQSPRNMYQCQMGKQTMGTPGVALCHRSDNK
LYRLQTGQTPIVKANLYDDYGMDNFPNGFNAVVAVISYTGYDMDDAMIINKSADERGF
GYGTMYKTEKVDLALNRNRGDPITQHFGFGNDEWPKEWLEKLDEDGLPYIGTYVEEGD
PICAYFDDTLNKTKIKTYHSSEPAYIEEVNLIGDESNKFQELQTVSIKYRIRRTPQIG
DKFSSRHGQKGVCSRKWPTIDMPFSETGIQPDIIINPHAFPSRMTIGMFVESLAGKAG
ALHGIAQDSTPWIFNEDDTPADYFGEQLAKAGYNYHGNEPMYSGATGEELRADIYVGV
VYYQRLRHMVNDKFQVRSTGPVNSLTMQPVKGRKRHGGIRVGEMERDALIGHGTSFLL
QDRLLNSSDYTQASVCRECGSILTTQQSVPRIGSISTVCCRRCSMRFEDAKKLLTKSE
DGEKIFIDDSQIWEDGQGNKFVGGNETTTVAIPFVLKYLDSELSAMGIRLRYNVEPK"
gene 582062..582134
/locus_tag="YNCP0010W"
/db_xref="GeneID:856120"
tRNA 582062..582134
/locus_tag="YNCP0010W"
/product="tRNA-Lys"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:8915539]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:8915539]"
/experiment="EXISTENCE:mutant phenotype:GO:0030533 triplet
codon-amino acid adaptor activity [PMID:17560369]"
/experiment="EXISTENCE:mutant phenotype:GO:0032543
mitochondrial translation [PMID:17560369]"
/note="Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE
analysis; a small portion is imported into mitochondria
via interaction with mt lysyl-tRNA synthetase Msk1p and is
necessary to decode AAG codons at high temperature, when
base modification of mt-encoded tRNA-Lys is reduced"
/db_xref="GeneID:856120"
/db_xref="SGD:S000006628"
gene complement(<582373..>582734)
/gene="MRA1"
/locus_tag="YPR010C-A"
/gene_synonym="MIN8"
/db_xref="GeneID:5142379"
mRNA complement(join(<582373..582558,582702..>582734))
/gene="MRA1"
/locus_tag="YPR010C-A"
/gene_synonym="MIN8"
/product="Mra1p"
/transcript_id="NM_001184689.1"
/db_xref="GeneID:5142379"
CDS complement(join(582373..582558,582702..582734))
/gene="MRA1"
/locus_tag="YPR010C-A"
/gene_synonym="MIN8"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:38508182]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0031333 negative
regulation of protein-containing complex assembly
[PMID:38508182]"
/note="Protein that negatively regulates respiratory
complex assembly; associated with the inner mitochondrial
membrane; antagonistic with Rcf2p to control ETC assembly;
conserved among Saccharomyces sensu stricto species"
/codon_start=1
/product="Mra1p"
/protein_id="NP_001091638.1"
/db_xref="GeneID:5142379"
/db_xref="SGD:S000122558"
/translation="MRPAQLLLNTAKKTSGGYKIPVELTPLFLAVGVALCSGTYFTYK
KLRTDETLRLTGNPELSSLDEVLAKDKD"
gene complement(<583062..>584042)
/gene="MRX21"
/locus_tag="YPR011C"
/db_xref="GeneID:856121"
mRNA complement(<583062..>584042)
/gene="MRX21"
/locus_tag="YPR011C"
/product="Mrx21p"
/transcript_id="NM_001184108.1"
/db_xref="GeneID:856121"
CDS complement(583062..584042)
/gene="MRX21"
/locus_tag="YPR011C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0046964
3'-phosphoadenosine 5'-phosphosulfate transmembrane
transporter activity [PMID:24296033]"
/experiment="EXISTENCE:direct assay:GO:1902557 5'-adenylyl
sulfate transmembrane transporter activity
[PMID:24296033]"
/experiment="EXISTENCE:direct assay:GO:1902558 5'-adenylyl
sulfate transmembrane transport [PMID:24296033]"
/experiment="EXISTENCE:direct assay:GO:1902559
3'-phospho-5'-adenylyl sulfate transmembrane transport
[PMID:24296033]"
/note="Mitochondrial transporter; major substrates are
adenosine 5'-phosphosulfate (APS) and 3'-phospho-adenosine
5'-phosphosulfate (PAPS); member of the mitochondrial
carrier family; the authentic, non-tagged protein is
detected in highly purified mitochondria in
high-throughput studies"
/codon_start=1
/product="Mrx21p"
/protein_id="NP_015336.1"
/db_xref="GeneID:856121"
/db_xref="SGD:S000006215"
/translation="MAEVLTVLEQPNSIKDFLKQDSNIAFLAGGVAGAVSRTVVSPFE
RVKILLQVQSSTTSYNRGIFSSIRQVYHEEGTKGLFRGNGLNCIRIFPYSAVQFVVYE
ACKKKLFHVNGNNGQEQLTNTQRLFSGALCGGCSVVATYPLDLIKTRLSIQTANLSSL
NRSKAKSISKPPGIWQLLSETYRLEGGLRGLYRGVWPTSLGVVPYVALNFAVYEQLRE
FGVNSSDAQPSWKSNLYKLTIGAISGGVAQTITYPFDLLRRRFQVLAMGGNELGFRYT
SVWDALVTIGRAEGVSGYYKGLAANLFKVVPSTAVSWLVYEVVCDSVRNW"
gene complement(<584632..>585585)
/gene="CMR3"
/locus_tag="YPR013C"
/db_xref="GeneID:856123"
mRNA complement(<584632..>585585)
/gene="CMR3"
/locus_tag="YPR013C"
/product="Cmr3p"
/transcript_id="NM_001184110.1"
/db_xref="GeneID:856123"
CDS complement(584632..585585)
/gene="CMR3"
/locus_tag="YPR013C"
/experiment="EXISTENCE:direct assay:GO:0003677 DNA binding
[PMID:23012409]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:19158363]"
/note="Putative zinc finger protein; YPR013C is not an
essential gene"
/codon_start=1
/product="Cmr3p"
/protein_id="NP_015338.1"
/db_xref="GeneID:856123"
/db_xref="SGD:S000006217"
/translation="MNFSLSKQSSEKQSSYTDKSRSPNIGMCTVNYKSNLPLQVSAVD
QLSTGKGTQFYGHNSYKNERECYNSTKINLPPISSLLPNFENNTPPNVDSRVQFPPQQ
VYQSMNVVPIVNEIYTPISMNATSDQYPIYYTESQQPIPHSQSPHLTSSAPLMMPVMV
PTVYKPLTPYDKEPITIASEPNFTAISMASHPNAALELCHDRPKSVPPGYGVLPTMQE
ASNGRTKSEPGAVLNGSATFSDWKTDTRISSTKLRKQCPVCGKICSRPSTLKTHYLIH
TGDTPFKCTWEGCTKSFNVKSNMLRHLKSHERKRNKVLNTT"
gene complement(<587189..>587518)
/locus_tag="YPR014C"
/db_xref="GeneID:856124"
mRNA complement(<587189..>587518)
/locus_tag="YPR014C"
/product="uncharacterized protein"
/transcript_id="NM_001348886.1"
/db_xref="GeneID:856124"
CDS complement(587189..587518)
/locus_tag="YPR014C"
/note="hypothetical protein; conserved across S.
cerevisiae strains; YPR014C is not an essential gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335824.1"
/db_xref="GeneID:856124"
/db_xref="SGD:S000006218"
/translation="MSCIFSADLGVEYSCAESRITNLVLCILCIREEKAAPVVKKDKF
LFFIFLSSKENLFCEISSRSSVSEVKSLRAKNNFICPHAVIGFQGAKAQLGIRFPQLN
GLLRLQH"
gene 588998..589830
/gene="SUT418"
/locus_tag="YNCP0026W"
/db_xref="GeneID:84313898"
ncRNA 588998..589830
/ncRNA_class="other"
/gene="SUT418"
/locus_tag="YNCP0026W"
/product="SUT418"
/note="Stable Unannotated Transcript functional ncRNA;
improves endogenous invertase secretion when deleted"
/transcript_id="NR_188209.1"
/db_xref="GeneID:84313898"
/db_xref="SGD:S000345044"
gene complement(<590283..>591026)
/locus_tag="YPR015C"
/db_xref="GeneID:856125"
mRNA complement(<590283..>591026)
/locus_tag="YPR015C"
/product="uncharacterized protein"
/transcript_id="NM_001184112.1"
/db_xref="GeneID:856125"
CDS complement(590283..591026)
/locus_tag="YPR015C"
/experiment="EXISTENCE:mutant phenotype:GO:0000077 DNA
damage checkpoint signaling [PMID:18617996]"
/note="Putative zinc finger transcription factor; binds
DNA in sequence-specific manner; overexpression causes a
cell cycle delay or arrest"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015340.1"
/db_xref="GeneID:856125"
/db_xref="SGD:S000006219"
/translation="MWRTKTLESMLCSPMKCSSSNIGGSYAQSSKEVSNTTKREVHLP
PCSSIMHAPLTPEINQAALPPPAYHYAPSSLHQTEDPVWRSSPNSIIFSPVIATPQPF
PLTFVERQSCCPIYSTAASSYTAQSVPPSMQHFQEENHRAVSNEQYSLPNVHIGQNPG
TLLSQTQTDLDLIQKQLRAVVKLRKQCPICGKVCSRPSTLRTHYLIHTGDTPFKCTWE
HCNKSFNVKSNMLRHLRTHQKKIAKKKHQ"
gene complement(<592332..>593069)
/gene="TIF6"
/locus_tag="YPR016C"
/gene_synonym="CDC95"
/db_xref="GeneID:856126"
mRNA complement(<592332..>593069)
/gene="TIF6"
/locus_tag="YPR016C"
/gene_synonym="CDC95"
/product="translation initiation factor 6"
/transcript_id="NM_001184113.1"
/db_xref="GeneID:856126"
CDS complement(592332..593069)
/gene="TIF6"
/locus_tag="YPR016C"
/gene_synonym="CDC95"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11238882]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:17353896]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11238882]"
/experiment="EXISTENCE:direct assay:GO:0030687
preribosome, large subunit precursor
[PMID:17443350|PMID:11583614|PMID:16188229|PMID:23212245]"
/experiment="EXISTENCE:direct assay:GO:0043023 ribosomal
large subunit binding [PMID:9891075]"
/experiment="EXISTENCE:genetic interaction:GO:0000054
ribosomal subunit export from nucleus [PMID:17353896]"
/experiment="EXISTENCE:genetic interaction:GO:0006364 rRNA
processing [PMID:17353896]"
/experiment="EXISTENCE:mutant phenotype:GO:0000463
maturation of LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11238882]"
/experiment="EXISTENCE:mutant phenotype:GO:0000466
maturation of 5.8S rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11238882]"
/experiment="EXISTENCE:mutant phenotype:GO:0042273
ribosomal large subunit biogenesis
[PMID:10206977|PMID:11238882]"
/experiment="EXISTENCE:mutant phenotype:GO:1902626
assembly of large subunit precursor of preribosome
[PMID:22735702]"
/note="Constituent of 66S pre-ribosomal particles; has
similarity to human translation initiation factor 6
(eIF6); may be involved in the biogenesis and or stability
of 60S ribosomal subunits"
/codon_start=1
/product="translation initiation factor 6"
/protein_id="NP_015341.1"
/db_xref="GeneID:856126"
/db_xref="SGD:S000006220"
/translation="MATRTQFENSNEIGVFSKLTNTYCLVAVGGSENFYSAFEAELGD
AIPIVHTTIAGTRIIGRMTAGNRRGLLVPTQTTDQELQHLRNSLPDSVKIQRVEERLS
ALGNVICCNDYVALVHPDIDRETEELISDVLGVEVFRQTISGNILVGSYCSLSNQGGL
VHPQTSVQDQEELSSLLQVPLVAGTVNRGSSVVGAGMVVNDYLAVTGLDTTAPELSVI
ESIFRLQDAQPESISGNLRDTLIETYS"
gene complement(<593486..>593917)
/gene="DSS4"
/locus_tag="YPR017C"
/db_xref="GeneID:856128"
mRNA complement(<593486..>593917)
/gene="DSS4"
/locus_tag="YPR017C"
/product="guanine nucleotide exchange factor DSS4"
/transcript_id="NM_001184114.1"
/db_xref="GeneID:856128"
CDS complement(593486..593917)
/gene="DSS4"
/locus_tag="YPR017C"
/experiment="EXISTENCE:direct assay:GO:0005085
guanyl-nucleotide exchange factor activity [PMID:9218467]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762|PMID:9218467]"
/experiment="EXISTENCE:direct assay:GO:0008270 zinc ion
binding [PMID:9218467]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:9218467]"
/experiment="EXISTENCE:genetic interaction:GO:0006892
post-Golgi vesicle-mediated transport
[PMID:9218467|PMID:8429886]"
/experiment="EXISTENCE:mutant phenotype:GO:0006892
post-Golgi vesicle-mediated transport
[PMID:9218467|PMID:8429886]"
/experiment="EXISTENCE:physical interaction:GO:0006892
post-Golgi vesicle-mediated transport [PMID:9218467]"
/note="Guanine nucleotide dissociation stimulator for
Sec4p; functions in the post-Golgi secretory pathway;
binds zinc, found both on membranes and in the cytosol"
/codon_start=1
/product="guanine nucleotide exchange factor DSS4"
/protein_id="NP_015342.1"
/db_xref="GeneID:856128"
/db_xref="SGD:S000006221"
/translation="MSKATCSFEGCHSAVITINDDNIINLPEQVHSEFKLLENRTMRD
ATPSESNFLVVPDVWDFDNVGVSREIPSSILGDLSDKSDFVFEYGNSSWKIKKCLKYL
ICADCDKGPIGIICKVQDQTKNEERVLHLLSLRSLQIMGRN"
gene <594476..>596296
/gene="RLF2"
/locus_tag="YPR018W"
/gene_synonym="CAC1"
/db_xref="GeneID:856129"
mRNA <594476..>596296
/gene="RLF2"
/locus_tag="YPR018W"
/gene_synonym="CAC1"
/product="Rlf2p"
/transcript_id="NM_001184115.1"
/db_xref="GeneID:856129"
CDS 594476..596296
/gene="RLF2"
/locus_tag="YPR018W"
/gene_synonym="CAC1"
/experiment="EXISTENCE:direct assay:GO:0000775 chromosome,
centromeric region [PMID:11782447]"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:25602519]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9030688]"
/experiment="EXISTENCE:direct assay:GO:0006335 DNA
replication-dependent chromatin assembly [PMID:9030687]"
/experiment="EXISTENCE:direct assay:GO:0033186 CAF-1
complex [PMID:9030687]"
/experiment="EXISTENCE:direct assay:GO:0042393 histone
binding [PMID:16503640]"
/experiment="EXISTENCE:mutant phenotype:GO:0006335 DNA
replication-dependent chromatin assembly [PMID:9030688]"
/note="Largest subunit (p90) of the Chromatin Assembly
Complex (CAF-1); chromatin assembly by CAF-1 is important
for multiple processes including histone H3/H4
tetramerization, silencing at telomeres, mating type loci
and rDNA, maintenance of kinetochore structure,
deactivation of DNA damage checkpoint after DNA repair,
chromatin dynamics during transcription, and repression of
divergent noncoding transcription"
/codon_start=1
/product="Rlf2p"
/protein_id="NP_015343.1"
/db_xref="GeneID:856129"
/db_xref="SGD:S000006222"
/translation="MEQHLKSIPLQDDTKKKGILSFFQNTTTVKSNKFLTKEKDVITL
DDPKEDVSGPMIETVKQETMKSINKECADEMKTTPKKANAEDKLLCYKNSPIQSTKYD
RNTNKQVPNGNIIAIETKSRSSSPCSKRELSSSKKEEAKREKELKKQQRAEEKHRKEL
LRQEEKKKKELKVEEERQRRAELKKQKEEEKRRKEEARLEAKRRKEEERLKKEEEIRL
KEEAKERAQSRIGNFFKKLSDSNTPVVEKSDYEKFFLPFYAKDGVRVSNKWKLTKVEL
EGSKRKIDDELLNSKDKTSSDDLLNWLQSRRLPRGHKIKRKAVDVLQQMPLKEKTDDE
LQSLLAQVPHKYIKFYENVRPPFIGTYSMDFTLPPNDPFSTKGTGFNYDYDSDVEWVN
EEEEGEVDNLESGEEEEEEEDDEDVPSEGEFDGFLDSEENSDLDGLPCAKRKFVGPLI
PTICLKSNFENLSEENKRYLQQLKAEVIIETDGPIDPFKEPKTSSLPSKRSNSDLQAQ
TASQSQSPEKKQKAMITDPMDLLRLFDGVQDSTFSLGTVTEIAQKNLPQYNKQTIKNT
IKEYAIRSSGKGDLPRKWVIKDAQNWENLRANANMPTPSL"
gene <596750..>599551
/gene="MCM4"
/locus_tag="YPR019W"
/gene_synonym="CDC54; HCD21"
/db_xref="GeneID:856130"
mRNA <596750..>599551
/gene="MCM4"
/locus_tag="YPR019W"
/gene_synonym="CDC54; HCD21"
/product="MCM DNA helicase complex subunit MCM4"
/transcript_id="NM_001184116.1"
/db_xref="GeneID:856130"
CDS 596750..599551
/gene="MCM4"
/locus_tag="YPR019W"
/gene_synonym="CDC54; HCD21"
/EC_number="3.6.4.12"
/experiment="EXISTENCE:direct assay:GO:0003678 DNA
helicase activity [PMID:15723534]"
/experiment="EXISTENCE:direct assay:GO:0003688 DNA
replication origin binding [PMID:11756674|PMID:16824194]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10559985|PMID:10704410]"
/experiment="EXISTENCE:direct assay:GO:0005656 nuclear
pre-replicative complex [PMID:9335335|PMID:16824194]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:10704410|PMID:10559985]"
/experiment="EXISTENCE:direct assay:GO:0006260 DNA
replication [PMID:15723534|PMID:13679365]"
/experiment="EXISTENCE:direct assay:GO:0006267
pre-replicative complex assembly involved in nuclear cell
cycle DNA replication [PMID:16824194]"
/experiment="EXISTENCE:direct assay:GO:0009378 four-way
junction helicase activity [PMID:13679365]"
/experiment="EXISTENCE:direct assay:GO:0017116
single-stranded DNA helicase activity
[PMID:15723534|PMID:18657510]"
/experiment="EXISTENCE:direct assay:GO:0031261 DNA
replication preinitiation complex [PMID:9554851]"
/experiment="EXISTENCE:direct assay:GO:0042555 MCM complex
[PMID:12480933]"
/experiment="EXISTENCE:direct assay:GO:0043138 3'-5' DNA
helicase activity [PMID:13679365]"
/experiment="EXISTENCE:direct assay:GO:0043596 nuclear
replication fork [PMID:16103218]"
/experiment="EXISTENCE:direct assay:GO:0071162 CMG complex
[PMID:26524492]"
/experiment="EXISTENCE:direct assay:GO:0097373 MCM core
complex [PMID:13679365]"
/experiment="EXISTENCE:genetic interaction:GO:0006270 DNA
replication initiation [PMID:2044962]"
/experiment="EXISTENCE:mutant phenotype:GO:0000727
double-strand break repair via break-induced replication
[PMID:20516198]"
/experiment="EXISTENCE:mutant phenotype:GO:0003697
single-stranded DNA binding [PMID:17895243]"
/experiment="EXISTENCE:mutant phenotype:GO:0006271 DNA
strand elongation involved in DNA replication
[PMID:10834843]"
/experiment="EXISTENCE:mutant phenotype:GO:0033260 nuclear
DNA replication [PMID:2044962]"
/note="Essential helicase component of heterohexameric
MCM2-7 complexes; MCM2-7 complexes bind pre-replication
complexes on DNA and melt DNA prior to replication; forms
an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in
G1; homolog of S. pombe Cdc21p"
/codon_start=1
/product="MCM DNA helicase complex subunit MCM4"
/protein_id="NP_015344.1"
/db_xref="GeneID:856130"
/db_xref="SGD:S000006223"
/translation="MSQQSSSPTKEDNNSSSPVVPNPDSVPPQLSSPALFYSSSSSQG
DIYGRNNSQNLSQGEGNIRAAIGSSPLNFPSSSQRQNSDVFQSQGRQGRIRSSASASG
RSRYHSDLRSDRALPTSSSSLGRNGQNRVHMRRNDIHTSDLSSPRRIVDFDTRSGVNT
LDTSSSSAPPSEASEPLRIIWGTNVSIQECTTNFRNFLMSFKYKFRKILDEREEFINN
TTDEELYYIKQLNEMRELGTSNLNLDARNLLAYKQTEDLYHQLLNYPQEVISIMDQTI
KDCMVSLIVDNNLDYDLDEIETKFYKVRPYNVGSCKGMRELNPNDIDKLINLKGLVLR
STPVIPDMKVAFFKCNVCDHTMAVEIDRGVIQEPARCERIDCNEPNSMSLIHNRCSFA
DKQVIKLQETPDFVPDGQTPHSISLCVYDELVDSCRAGDRIEVTGTFRSIPIRANSRQ
RVLKSLYKTYVDVVHVKKVSDKRLDVDTSTIEQELMQNKVDHNEVEEVRQITDQDLAK
IREVAAREDLYSLLARSIAPSIYELEDVKKGILLQLFGGTNKTFTKGGRYRGDINILL
CGDPSTSKSQILQYVHKITPRGVYTSGKGSSAVGLTAYITRDVDTKQLVLESGALVLS
DGGVCCIDEFDKMSDSTRSVLHEVMEQQTISIAKAGIITTLNARSSILASANPIGSRY
NPNLPVTENIDLPPPLLSRFDLVYLVLDKVDEKNDRELAKHLTNLYLEDKPEHISQDD
VLPVEFLTMYISYAKEHIHPIITEAAKTELVRAYVGMRKMGDDSRSDEKRITATTRQL
ESMIRLAEAHAKMKLKNVVELEDVQEAVRLIRSAIKDYATDPKTGKIDMNLVQTGKSV
IQRKLQEDLSREIMNVLKDQASDSMSFNELIKQINEHSQDRVESSDIQEALSRLQQED
KVIVLGEGVRRSVRLNNRV"
gene <599870..>600217
/gene="ATP20"
/locus_tag="YPR020W"
/db_xref="GeneID:856131"
mRNA <599870..>600217
/gene="ATP20"
/locus_tag="YPR020W"
/product="F1F0 ATP synthase subunit g"
/transcript_id="NM_001184117.1"
/db_xref="GeneID:856131"
CDS 599870..600217
/gene="ATP20"
/locus_tag="YPR020W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0015986 proton
motive force-driven ATP synthesis [PMID:20691145]"
/experiment="EXISTENCE:direct assay:GO:0046933
proton-transporting ATP synthase activity, rotational
mechanism [PMID:20691145]"
/experiment="EXISTENCE:mutant phenotype:GO:0005739
mitochondrion [PMID:17761666|PMID:16551625|PMID:20219971]"
/experiment="EXISTENCE:mutant phenotype:GO:0015986 proton
motive force-driven ATP synthesis
[PMID:10336613|PMID:9857174]"
/experiment="EXISTENCE:mutant phenotype:GO:0042407 cristae
formation [PMID:19528297]"
/experiment="EXISTENCE:mutant phenotype:GO:0045259
proton-transporting ATP synthase complex [PMID:10336613]"
/experiment="EXISTENCE:mutant phenotype:GO:0065003
protein-containing complex assembly
[PMID:17761666|PMID:20219971|PMID:16551625]"
/note="Subunit g of the mitochondrial F1F0 ATP synthase;
reversibly phosphorylated on two residues;
unphosphorylated form is required for dimerization of the
ATP synthase complex, which in turn determines
oligomerization of the complex and the shape of inner
membrane cristae"
/codon_start=1
/product="F1F0 ATP synthase subunit g"
/protein_id="NP_015345.1"
/db_xref="GeneID:856131"
/db_xref="SGD:S000006224"
/translation="MLSRIQNYTSGLVSKANLLSSKALYYGKVGAEISKQIYLKEGLQ
PPTVAQFKSVYSNLYKQSLNFALKPTEVLSCLKNIQKNELLKYGAYGIQLIGFYSVGE
IIGRRKLVGYKHH"
gene complement(<600649..>603357)
/gene="AGC1"
/locus_tag="YPR021C"
/db_xref="GeneID:856132"
mRNA complement(<600649..>603357)
/gene="AGC1"
/locus_tag="YPR021C"
/product="citrin"
/transcript_id="NM_001184118.1"
/db_xref="GeneID:856132"
CDS complement(600649..603357)
/gene="AGC1"
/locus_tag="YPR021C"
/experiment="EXISTENCE:direct assay:GO:0005313 L-glutamate
transmembrane transporter activity [PMID:14622413]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0015183 L-aspartate
transmembrane transporter activity [PMID:14622413]"
/experiment="EXISTENCE:direct assay:GO:0015292 uniporter
activity [PMID:14622413]"
/experiment="EXISTENCE:direct assay:GO:0015297 antiporter
activity [PMID:14622413]"
/experiment="EXISTENCE:direct assay:GO:0015810 aspartate
transmembrane transport [PMID:14622413]"
/experiment="EXISTENCE:direct assay:GO:0015813 L-glutamate
transmembrane transport [PMID:14622413]"
/experiment="EXISTENCE:direct assay:GO:1990816
vacuole-mitochondrion membrane contact site
[PMID:25026036]"
/experiment="EXISTENCE:mutant phenotype:GO:0009058
biosynthetic process [PMID:14622413]"
/note="Mitochondrial amino acid transporter; acts both as
a glutamate uniporter and as an aspartate-glutamate
exchanger; involved in nitrogen metabolism and nitrogen
compound biosynthesis; human homolog SLC25A13 complements
yeast null mutant"
/codon_start=1
/product="citrin"
/protein_id="NP_015346.1"
/db_xref="GeneID:856132"
/db_xref="SGD:S000006225"
/translation="MEQINSNSRKKKQQLEVFKYFASVLTKEDKPISISNGMLDMPTV
NSSKLTAGNGKPDTEKLTGELILTYDDFIELISSSKTIYSKFTDHSFNLNQIPKNVFG
CIFFAIDEQNKGYLTLNDWFYFNNLLEYDNYHLIILYEFFRKFDVENLKAKQKKELGS
SSFNLKAADDRIKSINYGNRFLSFDDLLLNLNQFKDTIRLLHESIDDNFVKDNKLLLD
WNDFRFLKFYKCYHENEEYLSLNSLVTILQNDLKNEKIFIGFDRLAQMDSQGHRLALS
KNQLTYLLRLFYSHRVSADIFSSLNLSNTELLKADNNSIPYNVFKDIFYLFQNFDLLN
QIFHKYVTENNLNEQDIREQIVTKNDFMTVLNAQYNKVNNIIEFSPSQINLLFSIVAN
SKENRRLRKRNQDRDDELLNDHHYDSDIDFFIHNEYLHGVSRSRKNLESFNDYYHDLS
DGFDQDSGVKKASKASTGLFESVFGGKKDKATMRSDLTIEDFMKILNPNYLNDLVHQM
ELQKNQNESLYINYYFYPIFDSLYNFSLGSIAGCIGATVVYPIDFIKTRMQAQRSLAQ
YKNSIDCLLKIISREGIKGLYSGLGPQLIGVAPEKAIKLTVNDFMRNRLTDKNGKLSL
FPEIISGASAGACQVIFTNPLEIVKIRLQVQSDYVGENIQQANETATQIVKKLGLRGL
YNGVAACLMRDVPFSAIYFPTYAHLKKDLFDFDPNDKTKRNRLKTWELLTAGAIAGMP
AAFLTTPFDVIKTRLQIDPRKGETKYNGIFHAIRTILKEESFRSFFKGGGARVLRSSP
QFGFTLAAYELFKGFIPSPDNKLKSREGRKRFCIDDDAGNEETVVHSNGELPQQKFYS
DDRKHANYYYKSCQIAKTFIDLDNNFSRFDSSVYKNFQEHLRSING"
gene complement(<603911..>607312)
/gene="SDD4"
/locus_tag="YPR022C"
/db_xref="GeneID:856133"
mRNA complement(<603911..>607312)
/gene="SDD4"
/locus_tag="YPR022C"
/product="Sdd4p"
/transcript_id="NM_001184119.1"
/db_xref="GeneID:856133"
CDS complement(603911..607312)
/gene="SDD4"
/locus_tag="YPR022C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:19111667]"
/note="Putative transcription factor, as suggested by
computational analysis; green fluorescent protein
(GFP)-fusion protein localizes to both the cytoplasm and
the nucleus and is induced in response to the DNA-damaging
agent MMS; overproduction of a truncation allele
suppresses lethality due to expression of the dominant
PET9 allele AAC2-A128P"
/codon_start=1
/product="Sdd4p"
/protein_id="NP_015347.1"
/db_xref="GeneID:856133"
/db_xref="SGD:S000006226"
/translation="MHGKELAGRLRKRENDNDLSPNSSSSPAERFRCPHPECNKTFSR
QEHLSRHKLNHWPKEIYVCSYVLPTTNAPCNKTFVRKDLLIRHEKRHSKVKNRLSRPS
KDQISSSNKDFSKNAPYNPSEVPLSTQSGTSTINLIKNSVNPPPSITQESKFRPFLQQ
AQQPQQVQQSQQPQQIQQLQQLQFPQQLRAPLQQPMLQQQMHPQQASPTFPSYDPRIR
NNGQNGNQFFNLIFDNRTGVNGFEVDAANNNGNGNDQNMNINPAVQQQRYQDRNFASS
SYQQPLQPLTQDQQQEQYFQQQKLAQQQQQQQQQQQQQQQLPPQNPFGDPLTSSSSGA
NLSVMQDLFSTNFLNSDPLQSFMQELSEAPQVSIEDTFSDKNTIPPNEKPVQQDEGFQ
NPPVMFELPQDNIKIPKAQPKFNDNPSTSVKDNLSSQKLNINELKRRSSKDSGVGNNS
SLNYKEQLRHSMKSVPSFFHPDPLTKYKISKEKCQEMFSFVPELRYVSIESIHKSLKS
FWLNFHPQYGLLHKPSFHVDKQPAILNLALIMTGASFLGSEYREQISDPICGPLRWII
FSHADFQPPSKTYIIQSLLLVEGYEKTSTNRYLHERSFLHHGTTIQLLRRTPSLGGHP
LMVKTGKTSGENSIQDPQEVYKRWIDFEMLKRIAFYAFYMDTTHAVVFGYWNLFINSN
QIQLTLPCPDQVWESYDLSYETLMEHGYGSTKRDENNTFLSALMQLMKNVIQILRNNN
IRRNKVNNGGIESTPTDLESTTDWNIQSLFGKKILLAGIISILFQCQEEVNGDYFITN
FRGGITDHLGLSWKDILSFAMNYWLHEVQKSCTDPKACRISTPSEETLTNRKIDEDNG
DGLCDDDLDLLSSDNPSNCKIPVIHISQIVLRILHHDYYIYAGAPWRMNVPIGRDEYD
MISRRILQFAKDPYNGGVAVVYAFQFLFEMFIIKENNVPTVVKSYNINSDPVITRPYA
IALTSLLIWSCNFALHGCEVSIWDNTEASKDECFQPDDSNGGNILGNTDNNGSTIANN
NLKEKNNYIPIESFEVYLLRMYRNLYVDSSLDVVSFQNDVWAKASLLQHISNTHFLCG
MMQFMRDIFNKSYWDLGREFGKLFDNCLERSLGKTSPTCHNMFDV"
gene complement(<608826..>610031)
/gene="EAF3"
/locus_tag="YPR023C"
/db_xref="GeneID:856134"
mRNA complement(<608826..>610031)
/gene="EAF3"
/locus_tag="YPR023C"
/product="Eaf3p"
/transcript_id="NM_001184120.1"
/db_xref="GeneID:856134"
CDS complement(608826..610031)
/gene="EAF3"
/locus_tag="YPR023C"
/experiment="EXISTENCE:direct assay:GO:0000123 histone
acetyltransferase complex [PMID:11036083]"
/experiment="EXISTENCE:direct assay:GO:0006281 DNA repair
[PMID:16135807]"
/experiment="EXISTENCE:direct assay:GO:0032221 Rpd3S
complex [PMID:16286007|PMID:16286008]"
/experiment="EXISTENCE:direct assay:GO:0035267 NuA4
histone acetyltransferase complex [PMID:15485911]"
/experiment="EXISTENCE:direct assay:GO:0140003 histone
H3K36me3 reader activity [PMID:16364921]"
/experiment="EXISTENCE:genetic interaction:GO:0006368
transcription elongation by RNA polymerase II
[PMID:19948887]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:11036083]"
/experiment="EXISTENCE:mutant phenotype:GO:0030174
regulation of DNA-templated DNA replication initiation
[PMID:19417103]"
/experiment="EXISTENCE:mutant phenotype:GO:0043487
regulation of RNA stability [PMID:31694990]"
/experiment="EXISTENCE:mutant phenotype:GO:0045892
negative regulation of DNA-templated transcription
[PMID:16364921]"
/experiment="EXISTENCE:mutant phenotype:GO:0060195
negative regulation of antisense RNA transcription
[PMID:21248844]"
/note="Component of the Rpd3S histone deacetylase complex;
Esa1p-associated factor, nonessential component of the
NuA4 acetyltransferase complex, homologous to Drosophila
dosage compensation protein MSL3; plays a role in
regulating Ty1 transposition"
/codon_start=1
/product="Eaf3p"
/protein_id="NP_015348.1"
/db_xref="GeneID:856134"
/db_xref="SGD:S000006227"
/translation="MVDLEQEFALGGRCLAFHGPLMYEAKILKIWDPSSKMYTSIPND
KPGGSSQATKEIKPQKLGEDESIPEEIINGKCFFIHYQGWKSSWDEWVGYDRIRAYNE
ENIAMKKRLANEAKEAKKSLLEQQKKKKLSTSLGGPSNGGKRKGDSRSNASISKSTSQ
SFLTSSVSGRKSGRSSANSLHPGSSLRSSSDQNGNDDRRRSSSLSPNMLHHIAGYPTP
KISLQIPIKLKSVLVDDWEYVTKDKKICRLPADVTVEMVLNKYEHEVSQELESPGSQS
QLSEYCAGLKLYFDKCLGNMLLYRLERLQYDELLKKSSKDQKPLVPIRIYGAIHLLRL
ISVLPELISSTTMDLQSCQLLIKQTEDFLVWLLMHVDEYFNDKDPNRSDDALYVNTSS
QYEGVALGM"
gene <610481..>612724
/gene="YME1"
/locus_tag="YPR024W"
/gene_synonym="OSD1; YTA11"
/db_xref="GeneID:856135"
mRNA <610481..>612724
/gene="YME1"
/locus_tag="YPR024W"
/gene_synonym="OSD1; YTA11"
/product="i-AAA protease YME1"
/transcript_id="NM_001184121.1"
/db_xref="GeneID:856135"
CDS 610481..612724
/gene="YME1"
/locus_tag="YPR024W"
/gene_synonym="OSD1; YTA11"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:16823961|PMID:14576278|PMID:24769239|PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:8688560|PMID:29138251]"
/experiment="EXISTENCE:direct assay:GO:0031942 i-AAA
complex [PMID:16267274]"
/experiment="EXISTENCE:direct assay:GO:0033619 membrane
protein proteolysis [PMID:29138251]"
/experiment="EXISTENCE:direct assay:GO:0045041 protein
import into mitochondrial intermembrane space
[PMID:16966379]"
/experiment="EXISTENCE:genetic interaction:GO:0004176
ATP-dependent peptidase activity [PMID:8688560]"
/experiment="EXISTENCE:mutant phenotype:GO:0004176
ATP-dependent peptidase activity [PMID:15772085]"
/experiment="EXISTENCE:mutant phenotype:GO:0006457 protein
folding [PMID:22993211]"
/experiment="EXISTENCE:mutant phenotype:GO:0030150 protein
import into mitochondrial matrix [PMID:27398316]"
/experiment="EXISTENCE:mutant phenotype:GO:0045041 protein
import into mitochondrial intermembrane space
[PMID:16966379]"
/experiment="EXISTENCE:mutant phenotype:GO:0051604 protein
maturation [PMID:16966379]"
/experiment="EXISTENCE:mutant phenotype:GO:0141164
mitochondrial protein quality control [PMID:18843051]"
/note="Catalytic subunit of i-AAA protease complex;
complex is located in mitochondrial inner membrane;
responsible for degradation of unfolded or misfolded
mitochondrial gene products; serves as nonconventional
translocation motor to pull PNPase into intermembrane
space; also has role in intermembrane space protein
folding; mutation causes elevated rate of mitochondrial
turnover; human homolog YME1L1 can complement yeast null
mutant"
/codon_start=1
/product="i-AAA protease YME1"
/protein_id="NP_015349.1"
/db_xref="GeneID:856135"
/db_xref="SGD:S000006228"
/translation="MNVSKILVSPTVTTNVLRIFAPRLPQIGASLLVQKKWALRSKKF
YRFYSEKNSGEMPPKKEADSSGKASNKSTISSIDNSQPPPPSNTNDKTKQANVAVSHA
MLATREQEANKDLTSPDAQAAFYKLLLQSNYPQYVVSRFETPGIASSPECMELYMEAL
QRIGRHSEADAVRQNLLTASSAGAVNPSLASSSSNQSGYHGNFPSMYSPLYGSRKEPL
HVVVSESTFTVVSRWVKWLLVFGILTYSFSEGFKYITENTTLLKSSEVADKSVDVAKT
NVKFDDVCGCDEARAELEEIVDFLKDPTKYESLGGKLPKGVLLTGPPGTGKTLLARAT
AGEAGVDFFFMSGSEFDEVYVGVGAKRIRDLFAQARSRAPAIIFIDELDAIGGKRNPK
DQAYAKQTLNQLLVELDGFSQTSGIIIIGATNFPEALDKALTRPGRFDKVVNVDLPDV
RGRADILKHHMKKITLADNVDPTIIARGTPGLSGAELANLVNQAAVYACQKNAVSVDM
SHFEWAKDKILMGAERKTMVLTDAARKATAFHEAGHAIMAKYTNGATPLYKATILPRG
RALGITFQLPEMDKVDITKRECQARLDVCMGGKIAEELIYGKDNTTSGCGSDLQSATG
TARAMVTQYGMSDDVGPVNLSENWESWSNKIRDIADNEVIELLKDSEERARRLLTKKN
VELHRLAQGLIEYETLDAHEIEQVCKGEKLDKLKTSTNTVVEGPDSDERKDIGDDKPK
IPTMLNA"
gene complement(<613377..>614558)
/gene="CCL1"
/locus_tag="YPR025C"
/db_xref="GeneID:856136"
mRNA complement(<613377..>614558)
/gene="CCL1"
/locus_tag="YPR025C"
/product="TFIIH complex kinase subunit CCL1"
/transcript_id="NM_001184122.1"
/db_xref="GeneID:856136"
CDS complement(613377..614558)
/gene="CCL1"
/locus_tag="YPR025C"
/experiment="EXISTENCE:direct assay:GO:0005675
transcription factor TFIIH holo complex [PMID:19818408]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0006366
transcription by RNA polymerase II [PMID:19818408]"
/experiment="EXISTENCE:direct assay:GO:0070985
transcription factor TFIIK complex
[PMID:11839796|PMID:19818408]"
/experiment="EXISTENCE:genetic interaction:GO:0016538
cyclin-dependent protein serine/threonine kinase regulator
activity [PMID:8230216]"
/experiment="EXISTENCE:mutant phenotype:GO:0010508
positive regulation of autophagy [PMID:26567215]"
/note="Cyclin partner of the cyclin-dependent kinase (CDK)
Kin28p; regulates the activity of Kin28p, a
TFIIH-associated carboxy-terminal domain (CTD) kinase that
facilitates recruitment of mRNA 5'-capping and
polyadenylation factors to the RNAPII holoenzyme complex,
and has a minor role in RNAPII transcription; subunit of
TFIIK, a TFIIH subassembly; human homolog CCNH allows
growth of a yeast ccl1 temperature-sensitive mutant at
restrictive temperature"
/codon_start=1
/product="TFIIH complex kinase subunit CCL1"
/protein_id="NP_015350.1"
/db_xref="GeneID:856136"
/db_xref="SGD:S000006229"
/translation="MTDIQLNGKSTLDTPSATMSAKEKEAKLKSADENNKPPNYKRIS
DDDLYRHSSQYRMWSYTKDQLQEKRVDTNARAIAYIEENLLKFREAHNLTEEEIKVLE
AKAIPLTMEEELDLVNFYAKKVQVIAQHLNLPTEVVATAISFFRRFFLENSVMQIDPK
SIVHTTIFLACKSENYFISVDSFAQKAKSTRDSVLKFEFKLLESLKFSLLNHHPYKPL
HGFFLDIQNVLYGKVDLNYMGQIYDRCKKRITAALLTDVVYFYTPPQITLATLLIEDE
ALVTRYLETKFPSREGSQESVPGNEKEEPQNDASTTEKNKEKSTESEEYSIDSAKLLT
IIRECKSIIEDCKPPSTEEAKKIAAKNYYCQNPSTLIQKLKRKLNGEDTSSTVEKKQK
T"
gene <615379..>619014
/gene="ATH1"
/locus_tag="YPR026W"
/db_xref="GeneID:856137"
mRNA <615379..>619014
/gene="ATH1"
/locus_tag="YPR026W"
/product="alpha,alpha-trehalase ATH1"
/transcript_id="NM_001184123.1"
/db_xref="GeneID:856137"
CDS 615379..619014
/gene="ATH1"
/locus_tag="YPR026W"
/EC_number="3.2.1.28"
/experiment="EXISTENCE:direct assay:GO:0005993 trehalose
catabolic process [PMID:8764988]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:15128531]"
/experiment="EXISTENCE:direct assay:GO:0030287 cell
wall-bounded periplasmic space [PMID:15128531]"
/experiment="EXISTENCE:mutant phenotype:GO:0000324
fungal-type vacuole [PMID:7502577]"
/experiment="EXISTENCE:mutant phenotype:GO:0004555
alpha,alpha-trehalase activity [PMID:7502577]"
/experiment="EXISTENCE:mutant phenotype:GO:0005993
trehalose catabolic process [PMID:8764988|PMID:7502577]"
/experiment="EXISTENCE:mutant phenotype:GO:0015771
trehalose transport [PMID:19016884]"
/note="Acid trehalase required for utilization of
extracellular trehalose; involved in intracellular
trehalose degradation during growth recovery after saline
stress"
/codon_start=1
/product="alpha,alpha-trehalase ATH1"
/protein_id="NP_015351.1"
/db_xref="GeneID:856137"
/db_xref="SGD:S000006230"
/translation="MKRIRSLWFNAEASYSNLNNSPSLRNKNSTGNNSRSKNYRSFSR
FDLINSILLLMMLFLLAIFVTALYLTKSSRLTYSHASRAALFNPLGVISPSLGNHTLN
YDPEARESSKKLYELLSDFNTAYYDDENMILGSNLFSKNTYSRQPYVANGYIGSRIPN
IGFGYALDTLNFYTDAPGALNNGWPLRNHRFAGAFVSDFYCLQPKLNSTNFPELDDVG
YSTVISSIPQWTNLQFSLVNDSKWFNPQNVTLDDVTNYSQNLSMKDGIVTTELDWLNS
QIHVKSEIWAHRHIHPLGVVSLEISLNTDHLPSDFDSLDVNIWDILDFNTSHRTVLHS
TGTDEKNNAVFMIVQPDNVPSSNCAIYSTCTVKYENSTNPINSSESFEEKDVSSNIYN
VILTEDQPKIIVHKYVGIMSTEFNKNKEQQDNTNIGLAKMIALNSKGNYEKLLSSHKR
AWYDLYNDAFIEIPSDSLLEMTARSSLFHLLANTRDYNVSSDRGLPVGVSGLSSDSYG
GMVFWDADIWMEPALLPFFPNVAQNMNNYRNATHSQAKLNAEKYGYPGAIYPWTSGKY
ANCTSTGPCVDYEYHINVDVAMASFSIYLNGHEGIDDEYLRYTTWPIIKNAAQFFTAY
VKYNSSLGLYETYNLTDPDEFANHINNGAFTNAGIKTLLKWATDIGNHLGEVVDPKWS
EISKDIYIPRSSSNITLEYSGMNSSVEIKQADVTLMVYPLGYINDESILNNAIKDLYY
YSERQSASGPAMTYPVFVAAAAGLLNHGSSSQSYLYKSVLPYLRAPFAQFSEQSDDNF
LTNGLTQPAFPFLTANGGFLQSILFGLTGIRYSYEVDPDTKKINRLLRFNPIELPLLP
GGIAIRNFKYMNQVLDIIIDDHNGTIVHKSGDVPIHIKIPNRSLIHDQDINFYNGSEN
ERKPNLERRDVDRVGDPMRMDRYGTYYLLKPKQELTVQLFKPGLNARNNIAENKQITN
LTAGVPGDVAFSALDGNNYTHWQPLDKIHRAKLLIDLGEYNEKEITKGMILWGQRPAK
NISISILPHSEKVENLFANVTEIMQNSGNDQLLNETIGQLLDNAGIPVENVIDFDGIE
QEDDESLDDVQALLHWKKEDLAKLIEQIPRLNFLKRKFVKILDNVPVSPSEPYYEASR
NQSLIEILPSNRTTFTIDYDKLQVGDKGNTDWRKTRYIVVAVQGVYDDYDDDNKGATI
KEIVLND"
gene complement(<620425..>621258)
/locus_tag="YPR027C"
/db_xref="GeneID:856138"
mRNA complement(<620425..>621258)
/locus_tag="YPR027C"
/product="uncharacterized protein"
/transcript_id="NM_001184124.1"
/db_xref="GeneID:856138"
CDS complement(620425..621258)
/locus_tag="YPR027C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/note="hypothetical protein; SWAT-GFP and seamless-GFP
fusion proteins localize to the endoplasmic reticulum,
while the mCherry fusion protein localizes to the vacuole"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015352.1"
/db_xref="GeneID:856138"
/db_xref="SGD:S000006231"
/translation="MVGIYRILASFVPLLGLLFAFHDDDMIDTVTIIKTVYETVTSTS
TAPAPAATKSVSEKKLDDTKLTLQVIQTMVSCFSVGENPANMISCGLGVVILMFSLII
ELINKLENDGINEPQRLYDLIKPKYVELPSNYVNEKIKTTFEPLDLYLGVNMNTSGSE
LNQNCLILKLGEKTALPFPGLAQQICYTKGASNEFTNYKLSDIQGNLNENSQGIANGV
FQKISNIRKISGNFKSQLYQISEKITDENWDGSAVGFTAHGREKGPNKSQISVSFYRD
N"
gene complement(622540..622631)
/locus_tag="YNCP0011C"
/db_xref="GeneID:856139"
tRNA complement(join(622540..622575,622595..622631))
/locus_tag="YNCP0011C"
/product="tRNA-Phe"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:10024171]"
/note="Phenylalanine tRNA (tRNA-Phe), predicted by
tRNAscan-SE analysis"
/db_xref="GeneID:856139"
/db_xref="SGD:S000006571"
gene <623527..>624202
/gene="YOP1"
/locus_tag="YPR028W"
/gene_synonym="YIP2"
/db_xref="GeneID:856140"
mRNA join(<623527..623577,623711..>624202)
/gene="YOP1"
/locus_tag="YPR028W"
/gene_synonym="YIP2"
/product="Yop1p"
/transcript_id="NM_001184125.1"
/db_xref="GeneID:856140"
CDS join(623527..623577,623711..624202)
/gene="YOP1"
/locus_tag="YPR028W"
/gene_synonym="YIP2"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum
[PMID:26928762|PMID:27831485|PMID:16469703|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005933 cellular
bud [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:11278413]"
/experiment="EXISTENCE:direct assay:GO:0071782 endoplasmic
reticulum tubular network [PMID:16469703]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0007029
endoplasmic reticulum organization [PMID:19665976]"
/experiment="EXISTENCE:genetic interaction:GO:0032581
ER-dependent peroxisome organization [PMID:27129769]"
/experiment="EXISTENCE:genetic interaction:GO:0034976
response to endoplasmic reticulum stress [PMID:27117666]"
/experiment="EXISTENCE:genetic interaction:GO:0048309
endoplasmic reticulum inheritance [PMID:27117666]"
/experiment="EXISTENCE:genetic interaction:GO:0051292
nuclear pore complex assembly [PMID:19273614]"
/experiment="EXISTENCE:genetic interaction:GO:0071786
endoplasmic reticulum tubular network organization
[PMID:18309084|PMID:18442980|PMID:16469703]"
/experiment="EXISTENCE:mutant phenotype:GO:0016192
vesicle-mediated transport [PMID:11278413|PMID:12427979]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:11278413]"
/note="Reticulon-interacting protein; ER integral membrane
protein involved in the generation of tubular ER
morphology; promotes membrane curvature; forms tubules in
vitro; regulates the ER asymmetry-induced inheritance
block during ER stress; role in ER-derived peroxisomal
biogenesis; interacts with Yip1p to mediate membrane
traffic and with Sey1p to maintain ER morphology;
facilitates lipid exchange between the ER and
mitochondria; forms ER foci upon DNA replication stress"
/codon_start=1
/product="Yop1p"
/protein_id="NP_015353.1"
/db_xref="GeneID:856140"
/db_xref="SGD:S000006232"
/translation="MSEYASSIHSQMKQFDTKYSGNRILQQLENKTNLPKSYLVAGLG
FAYLLLIFINVGGVGEILSNFAGFVLPAYLSLVALKTPTSTDDTQLLTYWIVFSFLSV
IEFWSKAILYLIPFYWFLKTVFLIYIALPQTGGARMIYQKIVAPLTDRYILRDVSKTE
KDEIRASVNEASKATGASVH"
gene complement(<624469..>626967)
/gene="APL4"
/locus_tag="YPR029C"
/db_xref="GeneID:856141"
mRNA complement(<624469..>626967)
/gene="APL4"
/locus_tag="YPR029C"
/product="AP-1 complex subunit gamma"
/transcript_id="NM_001184126.1"
/db_xref="GeneID:856141"
CDS complement(624469..626967)
/gene="APL4"
/locus_tag="YPR029C"
/experiment="EXISTENCE:direct assay:GO:0030121 AP-1
adaptor complex [PMID:10564262]"
/experiment="EXISTENCE:direct assay:GO:0030276 clathrin
binding [PMID:11489214]"
/experiment="EXISTENCE:mutant phenotype:GO:0006896 Golgi
to vacuole transport [PMID:17003107]"
/note="Gamma-adaptin; large subunit of the
clathrin-associated protein (AP-1) complex; binds
clathrin; involved in vesicle mediated transport"
/codon_start=1
/product="AP-1 complex subunit gamma"
/protein_id="NP_015354.1"
/db_xref="GeneID:856141"
/db_xref="SGD:S000006233"
/translation="MGSSLRSFIKDVRGAKTLADERAIITKQSAKIRTKLRDDHLPHE
KRRVNIQKLLYLYILGEKTHFGQVESINLIASDDFVDKRLGYLAATLLLDESEDLLTL
LTNMLNNDLHHPNKYAVSLALTSLGFLSSPELARDLYPDVENIIKNSRDPFLLKKALQ
CAAKLIFKDVSLLEIFNIEDITKILSSHSICTHGVLLGVTKIIQSILLIGLNRKKDED
EDEDGIDYSNDILSPLSLLLRDFFIRLENMNSKNIEPGYDVQGICDPFLQCEIIYTLK
LYFQVGELLNSNNVLDYKDNFCDLLTRIATNTDSTKNSGQAILYETVKTIFSLDLNQP
LRVLGINILAKFLAGKDNNTKYVSLNTLLKVVPQEPTAVQRHRKFISHCLQDTDVSIR
MRALELSFAILDDSNLVELVNELMKFLAKQDEDSKDLIIYTIDHLIDTFDTRVVKDES
WKLDVFFNILKLVGSFINYEKINDILIIINNTSQLSDKSEFLRKMLTISLNGTSAEIS
EENIGWQLVLIWCIGEYGDLVLNEGNKNGADIINESSITDYLLTLQELYTATNLKIIN
YILTAALKLSVRFHDAKNIEKLRQLILSYTDSTDLSLQMKSNQYEIFFNQSISVKKII
LETMPKFEKITEEQDNGKALSKNLISNEPVDLLSDLLGEDSKAESKASTGDNVKPIDI
LEEIFGEKNDIAQVPKNANKEESINHSSAVEANSGVTLPLDANKIYDSSSLNVYASLL
SANSGLAHLDLYFQAKSLISDLKTFCAVPKAQKLTLGQLYPSSTINASQICKQSLKIS
GSGKLKLRVKLDFHLNGSSSITNEQFDHKFDETL"
gene <627880..>631245
/gene="CSR2"
/locus_tag="YPR030W"
/gene_synonym="ART8; MRG19"
/db_xref="GeneID:856142"
mRNA <627880..>631245
/gene="CSR2"
/locus_tag="YPR030W"
/gene_synonym="ART8; MRG19"
/product="Csr2p"
/transcript_id="NM_001184127.1"
/db_xref="GeneID:856142"
CDS 627880..631245
/gene="CSR2"
/locus_tag="YPR030W"
/gene_synonym="ART8; MRG19"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12444972]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:12444972]"
/experiment="EXISTENCE:genetic interaction:GO:0031505
fungal-type cell wall organization [PMID:10679005]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:12444972]"
/experiment="EXISTENCE:physical interaction:GO:0031625
ubiquitin protein ligase binding [PMID:18976803]"
/note="Nuclear ubiquitin protein ligase binding protein;
may regulate utilization of nonfermentable carbon sources
and endocytosis of plasma membrane proteins;
overproduction suppresses chs5 spa2 lethality at high
temp; ubiquitinated by Rsp5p, deubiquitinated by Ubp2p;
CSR2 has a paralog, ECM21, that arose from the whole
genome duplication"
/codon_start=1
/product="Csr2p"
/protein_id="NP_015355.1"
/db_xref="GeneID:856142"
/db_xref="SGD:S000006234"
/translation="MQSTVPIAIASNGNKRDVVQNVSAGDEGDILQRLARNREMISTS
LSPQKSSGFSGRRRSSSVRDALSSFFGTGNSPTSSMDDYSNLMNRNYSTASTAMCRGN
SFPSDVGTKAYNITGSYQPDRHRNSVPYTTIDQLHTRQDTGLRRESDPVAAKQISSNN
DIVRSFITHHASNSTMFINRVLSDYLADRGFIKQTPLYNKKSVLEISIATSAESVFLP
TTKSDETEYLSLIHGSLNQARTQPVGSTNTAESDFLPSCPTMDTLNENNDLSLFPLHT
QRTSPSNTARTGNAMDTSNSDRASPASNNNTTDADSFVASGNNNPMNNNNSPARNRHP
NSHSRSLPNAWNSQMPSFSFALIFSLNKSTTLSDIKVELTSNVRVVWFNGLPPTKNVN
EECYNIGSLDWTLNADNFNLFIPQGAKSPLDIVENHSNNRKLKVLQKLSMRKRRSFSN
KAVLRENILNNLNASNSTNKLNAGVYVFTIPIVLASRIPESLYYPSARVSYSLRLATK
LKDEHTQLVASRPRSSSISSPQKLRSYSCSDSYEYSQIDDTIEGETYNNDKNSTGKIA
FPSSWLKSAKGRLKRNNSNGRSDNNGASSSGLAMQHDSEDTINLQYPLNLVRTPPEIS
VTTANKPLYINKVWENCLSYEISFAQKYVPLNGEIPITIKVAPLVKSLSVKRIRVSCR
EKISYRSKDYQYDFDQLDPLASDPCNPYHMRYLVRKKKDRSLPLFEVASKCTSGPSIR
EEVVTNTVDDNLLAYTSSKENNKDIPFSESFTVKTKLKFPKYCEVDATKAASLPPYGI
DLFDPIKDPTQSENTSNNGNVLGFLVGRPNRASKTVHKIPQDKNHNEVNDTNGNSNTS
LQTSSNVPIQHYTRLNKPRRGLYLDSMHFKNIQCSHKLEIVLRVSKTDSGSSKIIRHY
EVIVDTPIYLISDLCNTSNIDLPTYDMATTESSKVLPPTFEEATSVSASPRSSVSYYP
DDISMQQLNLSRSTSLANGYLSTLHPKTTAVSDSSNGAPIRDQQEQQARPLRTEDYAL
QMGNENNAYSNMDGLLSQDIFEQETAATLFKRDIVTMNFNNNIFTPRYSPRTFTNTDY
NYNDNDNNDNDTEGPGPIIHPGPEPPRYDEISS"
gene <631515..>633761
/gene="NTO1"
/locus_tag="YPR031W"
/db_xref="GeneID:856143"
mRNA <631515..>633761
/gene="NTO1"
/locus_tag="YPR031W"
/product="Nto1p"
/transcript_id="NM_001184128.1"
/db_xref="GeneID:856143"
CDS 631515..633761
/gene="NTO1"
/locus_tag="YPR031W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0033100 NuA3
histone acetyltransferase complex [PMID:17157260]"
/experiment="EXISTENCE:direct assay:GO:0140003 histone
H3K36me3 reader activity [PMID:17142463]"
/experiment="EXISTENCE:direct assay:GO:1990467 NuA3a
histone acetyltransferase complex [PMID:25104842]"
/experiment="EXISTENCE:direct assay:GO:1990468 NuA3b
histone acetyltransferase complex [PMID:25104842]"
/experiment="EXISTENCE:physical interaction:GO:0006338
chromatin remodeling [PMID:17157260]"
/note="Subunit of the NuA3 histone acetyltransferase
complex; this complex acetylates histone H3; contains PHD
finger domain that interacts with methylated histone H3"
/codon_start=1
/product="Nto1p"
/protein_id="NP_015356.1"
/db_xref="GeneID:856143"
/db_xref="SGD:S000006235"
/translation="MNRGSLDDGPKLREEKHFQDFYPDLNADTLLPFIVPLVETKDNS
TDTDSDDISNRNNREIGSVKSVQTKELIFKGRVTTEPLVLKKNEVEFQKCKITTNELK
GKKNPYCVRFNESFISRYYHINKVRNRKSYKQQQKEFDGVEAPYFTKFSSKEAPNITI
STSTKSAIQKFASISPNLVNFKPQYDMDEQDELYLHYLNKRYFKDQMSHEIFEILMTT
LETEWFHIEKHIPSTNSLIARHNILRDCKNYELYGSDDGTGLSMDQACAVCLGTDSDN
LNTIVFCDGCDIAVHQECYGIIFIPEGKWLCRRCMISKNNFATCLMCPSHTGAFKQTD
TGSWVHNICALWLPELYFSNLHYMEPIEGVQNVSVSRWKLNCYICKKKMGACIQCFQR
NCFTAYHVTCARRAGLYMSKGKCTIQELASNQFSQKYSVESFCHKHAPRGWQTSIEGI
NKARKYFSLLSTLQTETPQHNEANDRTNSKFNKTIWKTPNQTPVAPHVFAEILQKVVD
FFGLANPPAGAFDICKYWSMKRELTGGTPLTACFENNSLGSLTEEQVQTRIDFANDQL
EDLYRLKELTTLVKKRTQASNSLSRSRKKVFDIVKSPQKYLLKINVLDIFIKSEQFKA
LERLVTEPKLLVILEKCKHCDFDTVQIFKEEIMHFFEVLETLPGASRILQTVSSKAKE
QVTNLIGLIEHVDIKKLLSRDFIINDDKIEERPWSGPVIMEEEGLSDAEELSAGEHRM
LKLILNSG"
rep_origin 633874..634122
/note="ARS1623; Autonomously Replicating Sequence"
/db_xref="SGD:S000118419"
gene <634123..>637224
/gene="SRO7"
/locus_tag="YPR032W"
/gene_synonym="SNI1; SOP1"
/db_xref="GeneID:856144"
mRNA <634123..>637224
/gene="SRO7"
/locus_tag="YPR032W"
/gene_synonym="SNI1; SOP1"
/product="Rab GTPase-binding protein SRO7"
/transcript_id="NM_001184129.1"
/db_xref="GeneID:856144"
CDS 634123..637224
/gene="SRO7"
/locus_tag="YPR032W"
/gene_synonym="SNI1; SOP1"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:10402465|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:10402465]"
/experiment="EXISTENCE:direct assay:GO:0031267 small
GTPase binding [PMID:16390997]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:22832273]"
/experiment="EXISTENCE:mutant phenotype:GO:0006887
exocytosis [PMID:10402465]"
/experiment="EXISTENCE:mutant phenotype:GO:0006893 Golgi
to plasma membrane transport [PMID:10402465]"
/experiment="EXISTENCE:physical interaction:GO:0000149
SNARE binding [PMID:15964280]"
/experiment="EXISTENCE:physical interaction:GO:0007264
small GTPase-mediated signal transduction [PMID:16390997]"
/experiment="EXISTENCE:physical interaction:GO:0030010
establishment of cell polarity [PMID:15964280]"
/note="Effector of Rab GTPase Sec4p; forms a complex with
Sec4p and t-SNARE Sec9p; involved in exocytosis and
docking and fusion of post-Golgi vesicles with plasma
membrane; regulates cell proliferation and colony
development via the Rho1-Tor1 pathway; homolog of
Drosophila lgl tumor suppressor; SRO7 has a paralog,
SRO77, that arose from the whole genome duplication"
/codon_start=1
/product="Rab GTPase-binding protein SRO7"
/protein_id="NP_015357.1"
/db_xref="GeneID:856144"
/db_xref="SGD:S000006236"
/translation="MFGSKRLKNVKEAFKSLKGQNSETPIENSKASFKSKNSKTSTIS
KDAKSSSSLKIPISSNNKNKIFSLAETNKYGMSSKPIAAAFDFTQNLLAIATVTGEVH
IYGQQQVEVVIKLEDRSAIKEMRFVKGIYLVVINAKDTVYVLSLYSQKVLTTVFVPGK
ITSIDTDASLDWMLIGLQNGSMIVYDIDRDQLSSFKLDNLQKSSFFPAARLSPIVSIQ
WNPRDIGTVLISYEYVTLTYSLVENEIKQSFIYELPPFAPGGDFSEKTNEKRTPKVIQ
SLYHPNSLHIITIHEDNSLVFWDANSGHMIMARTVFETEINVPQPDYIRDSSTNAAKI
SKVYWMCENNPEYTSLLISHKSISRGDNQSLTMIDLGYTPRYSITSYEGMKNYYANPK
QMKIFPLPTNVPIVNILPIPRQSPYFAGCHNPGLILLILGNGEIETMLYPSGIFTDKA
SLFPQNLSWLRPLATTSMAASVPNKLWLGALSAAQNKDYLLKGGVRTKRQKLPAEYGT
AFITGHSNGSVRIYDASHGDIQDNASFEVNLSRTLNKAKELAVDKISFAAETLELAVS
IETGDVVLFKYEVNQFYSVENRPESGDLEMNFRRFSLNNTNGVLVDVRDRAPTGVRQG
FMPSTAVHANKGKTSAINNSNIGFVGIAYAAGSLMLIDRRGPAIIYMENIREISGAQS
ACVTCIEFVIMEYGDDGYSSILMVCGTDMGEVITYKILPASGGKFDVQLMDITNVTSK
GPIHKIDAFSKETKSSCLATIPKMQNLSKGLCIPGIVLITGFDDIRLITLGKSKSTHK
GFKYPLAATGLSYISTVEKNNDRKNLTVIITLEINGHLRVFTIPDFKEQMSEHIPFPI
AAKYITESSVLRNGDIAIRVSEFQASLFSTVKEQDTLAPVSDTLYINGIRIPYRPQVN
SLQWARGTVYCTPAQLNELLGGVNRPASKYKESIIAEGSFSERSSDDNNANHPEHQYT
KPTRKGRNSSYGVLRNVSRAVETRWDAVEDRFNDYATAMGETMNEAVEQTGKDVMKGA
LGF"
gene complement(<637379..>639019)
/gene="HTS1"
/locus_tag="YPR033C"
/gene_synonym="TS4572; TSM4572"
/db_xref="GeneID:856145"
mRNA complement(<637379..>639019)
/gene="HTS1"
/locus_tag="YPR033C"
/gene_synonym="TS4572; TSM4572"
/product="histidine--tRNA ligase"
/transcript_id="NM_001184130.1"
/db_xref="GeneID:856145"
CDS complement(637379..639019)
/gene="HTS1"
/locus_tag="YPR033C"
/gene_synonym="TS4572; TSM4572"
/EC_number="6.1.1.21"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0004812
aminoacyl-tRNA ligase activity [PMID:16741232]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0006427
histidyl-tRNA aminoacylation [PMID:16741232]"
/experiment="EXISTENCE:direct assay:GO:1990825
sequence-specific mRNA binding [PMID:34039240]"
/experiment="EXISTENCE:genetic interaction:GO:0032543
mitochondrial translation [PMID:16777356]"
/experiment="EXISTENCE:mutant phenotype:GO:0005737
cytoplasm [PMID:3521891]"
/experiment="EXISTENCE:mutant phenotype:GO:0005739
mitochondrion [PMID:3521891]"
/experiment="EXISTENCE:mutant phenotype:GO:0006427
histidyl-tRNA aminoacylation [PMID:1459448]"
/note="Cytoplasmic and mitochondrial histidine tRNA
synthetase; efficient mitochondrial localization requires
both a presequence and an amino-terminal sequence;
mutations in human ortholog HARS2 are associated with
Perrault syndrome"
/codon_start=1
/product="histidine--tRNA ligase"
/protein_id="NP_015358.1"
/db_xref="GeneID:856145"
/db_xref="SGD:S000006237"
/translation="MLSRSLNKVVTSIKSSSIIRMSSATAAATSAPTANAANALKASK
APKKGKLQVSLKTPKGTKDWADSDMVIREAIFSTLSGLFKKHGGVTIDTPVFELREIL
AGKYGEDSKLIYNLEDQGGELCSLRYDLTVPFARYVAMNNIQSIKRYHIAKVYRRDQP
AMTKGRMREFYQCDFDVAGTFESMVPDSECLSILVEGLTSLGIKDFKIKLNHRKILDG
IFQIAGVKDEDVRKISSAVDKLDKSPWEAVKKEMTEEKGQSEETADKIGEYVKLNGSL
KEIHAVLSADANITSNEKAKQGLDDIATLMKYTEAFDIDSFISFDLSLARGLDYYTGL
IYEVVTSASAPPENASELKKKAKSAEDASEFVGVGSIAAGGRYDNLVNMFSEASGKKS
TQIPCVGISFGVERIFSLIKQRINSSTTIKPTATQVFVMAFGGGKDWTGYLPERMKVT
KQLWDAGIEAEYVYKAKANPRKQFDAAEKAGCHIAVILGKEEYLEGKLRVKRLGQEFA
DDDGELVSAADIVPIVQEKLSQIHEDGLNEVTRLIKGL"
gene <639525..>640958
/gene="ARP7"
/locus_tag="YPR034W"
/gene_synonym="RSC11; SWP61"
/db_xref="GeneID:856146"
mRNA <639525..>640958
/gene="ARP7"
/locus_tag="YPR034W"
/gene_synonym="RSC11; SWP61"
/product="Arp7p"
/transcript_id="NM_001184131.1"
/db_xref="GeneID:856146"
CDS 639525..640958
/gene="ARP7"
/locus_tag="YPR034W"
/gene_synonym="RSC11; SWP61"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11011149]"
/experiment="EXISTENCE:direct assay:GO:0006337 nucleosome
disassembly [PMID:16492771]"
/experiment="EXISTENCE:direct assay:GO:0006338 chromatin
remodeling [PMID:12183366]"
/experiment="EXISTENCE:direct assay:GO:0006368
transcription elongation by RNA polymerase II
[PMID:17081996]"
/experiment="EXISTENCE:direct assay:GO:0016514 SWI/SNF
complex [PMID:9726966|PMID:8127913|PMID:18644858]"
/experiment="EXISTENCE:direct assay:GO:0016586 RSC-type
complex [PMID:12183366|PMID:10619019]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:9726966]"
/note="Component of both the SWI/SNF and RSC chromatin
remodeling complexes; actin-related protein involved in
transcriptional regulation"
/codon_start=1
/product="Arp7p"
/protein_id="NP_015359.1"
/db_xref="GeneID:856146"
/db_xref="SGD:S000006238"
/translation="MTLNRKCVVIHNGSHRTVAGFSNVELPQCIIPSSYIKRTDEGGE
AEFIFGTYNMIDAAAEKRNGDEVYTLVDSQGLPYNWDALEMQWRYLYDTQLKVSPEEL
PLVITMPATNGKPDMAILERYYELAFDKLNVPVFQIVIEPLAIALSMGKSSAFVIDIG
ASGCNVTPIIDGIVVKNAVVRSKFGGDFLDFQVHERLAPLIKEENDMENMADEQKRST
DVWYEASTWIQQFKSTMLQVSEKDLFELERYYKEQADIYAKQQEQLKQMDQQLQYTAL
TGSPNNPLVQKKNFLFKPLNKTLTLDLKECYQFAEYLFKPQLISDKFSPEDGLGPLMA
KSVKKAGASINSMKANTSTNPNGLGTSHINTNVGDNNSTASSSNISPEQVYSLLLTNV
IITGSTSLIEGMEQRIIKELSIRFPQYKLTTFANQVMMDRKIQGWLGALTMANLPSWS
LGKWYSKEDYETLKRDRKQSQATNATN"
gene <642208..>643320
/gene="GLN1"
/locus_tag="YPR035W"
/db_xref="GeneID:856147"
mRNA <642208..>643320
/gene="GLN1"
/locus_tag="YPR035W"
/product="glutamate--ammonia ligase"
/transcript_id="NM_001184132.2"
/db_xref="GeneID:856147"
CDS 642208..643320
/gene="GLN1"
/locus_tag="YPR035W"
/EC_number="6.3.1.2"
/experiment="EXISTENCE:direct assay:GO:0004356 glutamine
synthetase activity [PMID:4156034]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922|PMID:11914276|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006542 glutamine
biosynthetic process [PMID:6129248]"
/experiment="EXISTENCE:direct assay:GO:0034399 nuclear
periphery [PMID:25817432]"
/experiment="EXISTENCE:mutant phenotype:GO:0004356
glutamine synthetase activity [PMID:1347768]"
/note="Glutamine synthetase (GS); synthesizes glutamine
from glutamate and ammonia; with Glt1p, forms the
secondary pathway for glutamate biosynthesis from ammonia;
expression regulated by nitrogen source and by amino acid
limitation; forms filaments of back-to-back stacks of
cylindrical homo-decamers at low pH, leading to enzymatic
inactivation and storage during states of advanced
cellular starvation; relocalizes from nucleus to
cytoplasmic foci upon DNA replication stress"
/codon_start=1
/product="glutamate--ammonia ligase"
/protein_id="NP_015360.2"
/db_xref="GeneID:856147"
/db_xref="SGD:S000006239"
/translation="MAEASIEKTQILQKYLELDQRGRIIAEYVWIDGTGNLRSKGRTL
KKRITSIDQLPEWNFDGSSTNQAPGHDSDIYLKPVAYYPDPFRRGDNIVVLAACYNND
GTPNKFNHRHEAAKLFAAHKDEEIWFGLEQEYTLFDMYDDVYGWPKGGYPAPQGPYYC
GVGAGKVYARDMIEAHYRACLYAGLEISGINAEVMPSQWEFQVGPCTGIDMGDQLWMA
RYFLHRVAEEFGIKISFHPKPLKGDWNGAGCHTNVSTKEMRQPGGMKYIEQAIEKLSK
RHAEHIKLYGSDNDMRLTGRHETASMTAFSSGVANRGSSIRIPRSVAKEGYGYFEDRR
PASNIDPYLVTGIMCETVCGAIDNADMTKEFERESS"
gene <643836..>645272
/gene="VMA13"
/locus_tag="YPR036W"
/gene_synonym="CLS11"
/db_xref="GeneID:856148"
mRNA <643836..>645272
/gene="VMA13"
/locus_tag="YPR036W"
/gene_synonym="CLS11"
/product="H(+)-transporting V1 sector ATPase subunit H"
/transcript_id="NM_001184133.1"
/db_xref="GeneID:856148"
CDS 643836..645272
/gene="VMA13"
/locus_tag="YPR036W"
/gene_synonym="CLS11"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762|PMID:23708375]"
/experiment="EXISTENCE:mutant phenotype:GO:0007035
vacuolar acidification [PMID:18708638]"
/note="Subunit H of the V1 peripheral membrane domain of
V-ATPase; part of the electrogenic proton pump found
throughout the endomembrane system; serves as an activator
or a structural stabilizer of the V-ATPase; the V1
peripheral membrane domain of the vacuolar H+-ATPase
(V-ATPase) has eight subunits"
/codon_start=1
/product="H(+)-transporting V1 sector ATPase subunit H"
/protein_id="NP_015361.1"
/db_xref="GeneID:856148"
/db_xref="SGD:S000006240"
/translation="MGATKILMDSTHFNEIRSIIRSRSVAWDALARSEELSEIDASTA
KALESILVKKNIGDGLSSSNNAHSGFKVNGKTLIPLIHLLSTSDNEDCKKSVQNLIAE
LLSSDKYGDDTVKFFQEDPKQLEQLFDVSLKGDFQTVLISGFNVVSLLVQNGLHNVKL
VEKLLKNNNLINILQNIEQMDTCYVCIRLLQELAVIPEYRDVIWLHEKKFMPTLFKIL
QRATDSQLATRIVATNSNHLGIQLQYHSLLLIWLLTFNPVFANELVQKYLSDFLDLLK
LVKITIKEKVSRLCISIILQCCSTRVKQHKKVIKQLLLLGNALPTVQSLSERKYSDEE
LRQDISNLKEILENEYQELTSFDEYVAELDSKLLCWSPPHVDNGFWSDNIDEFKKDNY
KIFRQLIELLQAKVRNGDVNAKQEKIIIQVALNDITHVVELLPESIDVLDKTGGKADI
MELLNHSDSRVKYEALKATQAIIGYTFK"
gene <645950..>646153
/gene="SPO24"
/locus_tag="YPR036W-A"
/db_xref="GeneID:856149"
mRNA <645950..>646153
/gene="SPO24"
/locus_tag="YPR036W-A"
/product="Spo24p"
/transcript_id="NM_001184516.1"
/db_xref="GeneID:856149"
CDS 645950..646153
/gene="SPO24"
/locus_tag="YPR036W-A"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:25127041]"
/experiment="EXISTENCE:mutant phenotype:GO:0030437
ascospore formation [PMID:25127041]"
/note="Small (67 amino acids) protein involved in
sporulation; localizes to the prospore membrane;
phosphorylated during meiosis; a longer, 5'-extended mRNA
is also transcribed beginning in mid-meiosis, regulated by
two MSEs (middle sporulation elements), and includes an
uORF of 15 codons in its 5'-UTR; evidence transcription is
regulated by Pdr1p"
/codon_start=1
/product="Spo24p"
/protein_id="NP_690847.1"
/db_xref="GeneID:856149"
/db_xref="SGD:S000028425"
/translation="MVAFLELTSDVSQPFVIPSLSPVSQPSSRKNSDANVDDLNLAIA
NAALLDASASSRSHSRKNSLSLL"
gene complement(<646448..>647038)
/gene="ERV2"
/locus_tag="YPR037C"
/db_xref="GeneID:856152"
mRNA complement(<646448..>647038)
/gene="ERV2"
/locus_tag="YPR037C"
/product="flavin-linked sulfhydryl oxidase"
/transcript_id="NM_001184134.1"
/db_xref="GeneID:856152"
CDS complement(646448..647038)
/gene="ERV2"
/locus_tag="YPR037C"
/EC_number="1.8.3.2"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:11584268]"
/experiment="EXISTENCE:direct assay:GO:0016971
flavin-dependent sulfhydryl oxidase activity
[PMID:11584268]"
/experiment="EXISTENCE:direct assay:GO:0016972 thiol
oxidase activity [PMID:11313344]"
/experiment="EXISTENCE:direct assay:GO:0060904 regulation
of protein folding in endoplasmic reticulum
[PMID:11584268]"
/note="Flavin-linked sulfhydryl oxidase localized to the
ER lumen; involved in disulfide bond formation within the
endoplasmic reticulum (ER)"
/codon_start=1
/product="flavin-linked sulfhydryl oxidase"
/protein_id="NP_015362.1"
/db_xref="GeneID:856152"
/db_xref="SGD:S000006241"
/translation="MKQIVKRSHAIRIVAALGIIGLWMFFSSNELSIATPGLIKAKSG
IDEVQGAAAEKNDARLKEIEKQTIMPLMGDDKVKKEVGRASWKYFHTLLARFPDEPTP
EEREKLHTFIGLYAELYPCGECSYHFVKLIEKYPVQTSSRTAAAMWGCHIHNKVNEYL
KKDIYDCATILEDYDCGCSDSDGKRVSLEKEAKQHG"
gene <646836..>647195
/gene="IRC16"
/locus_tag="YPR038W"
/db_xref="GeneID:856150"
mRNA <646836..>647195
/gene="IRC16"
/locus_tag="YPR038W"
/product="Irc16p"
/transcript_id="NM_001395041.1"
/db_xref="GeneID:856150"
CDS 646836..647195
/gene="IRC16"
/locus_tag="YPR038W"
/experiment="EXISTENCE:mutant phenotype:GO:0006312 mitotic
recombination [PMID:18085829]"
/note="hypothetical protein; partially overlaps verified
gene ERV2/YPR037C but doesn't share phenotypes; also
overlaps dubious ORF YPR039W; null mutant displays
increased levels of spontaneous Rad52p foci, increased
biofilm information, different altered sensitivities to
various chemcicals"
/codon_start=1
/product="Irc16p"
/protein_id="NP_001381971.1"
/db_xref="GeneID:856150"
/db_xref="SGD:S000006242"
/translation="MVCFSISFNRASFFSAAAPCTSSIPDFALIRPGVAMDSSLDEKN
IHRPMIPNAATILMAWLLLTICFIPSFLAQSVQTFLFTNHQPSRLLVFITHVYCLMIK
PFVLYFWFLFPLRLPGG"
gene <647305..>648375
/gene="TIP41"
/locus_tag="YPR040W"
/db_xref="GeneID:856153"
mRNA <647305..>648375
/gene="TIP41"
/locus_tag="YPR040W"
/product="Tip41p"
/transcript_id="NM_001184137.1"
/db_xref="GeneID:856153"
CDS 647305..648375
/gene="TIP41"
/locus_tag="YPR040W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276|PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:1904262
negative regulation of TORC1 signaling [PMID:11741537]"
/note="Tap42p-interacting protein; negative regulator of
the TORC1 signaling pathway that activates the type
2A-related phosphatase Sit4p by binding and antagonizing
Tap42p, a SIT4 inhibitor; proposed to be part of a
feedback loop to amplify Sit4p activity when TORC1 is
inactivated; protein abundance increases in response to
DNA replication stress"
/codon_start=1
/product="Tip41p"
/protein_id="NP_015365.1"
/db_xref="GeneID:856153"
/db_xref="SGD:S000006244"
/translation="MSKRNTPPLRSSGINTIQINAAREMHAQTVRARRMPMPTSGITT
PSVQPTAAPATPPRHICNNPNNPQCLHCGSVIIPSPRATLPLEDNPSISINDWTISSR
KKPILNSQELDIWENEKLKGLTLPEMIFGNNYIRIENSKQHWSIEFNALDALKEVQLQ
DSGIRVAYSNDWINSKKRQNSTNGAQRFTNDVNDDSLNIIHKYDWTYTTRYKGTESSP
ESKFRLDNDQKLPLDKLAVHDKILFYDDMILFEDELADNGISILNVKIRVMNERLLLL
SRFFLRVDDVLVRVYDTRIYVEFDENVVIRESKEFEGKYQDVLAKHRLSQSHDPKAAL
RDSNWVAQNTPMIKRQCEIIQF"
gene <648704..>649921
/gene="TIF5"
/locus_tag="YPR041W"
/gene_synonym="SUI5"
/db_xref="GeneID:856154"
mRNA <648704..>649921
/gene="TIF5"
/locus_tag="YPR041W"
/gene_synonym="SUI5"
/product="translation initiation factor eIF5"
/transcript_id="NM_001184138.1"
/db_xref="GeneID:856154"
CDS 648704..649921
/gene="TIF5"
/locus_tag="YPR041W"
/gene_synonym="SUI5"
/experiment="EXISTENCE:direct assay:GO:0001732 formation
of cytoplasmic translation initiation complex
[PMID:12008673]"
/experiment="EXISTENCE:direct assay:GO:0003743 translation
initiation factor activity [PMID:12008673]"
/experiment="EXISTENCE:direct assay:GO:0005092
GDP-dissociation inhibitor activity [PMID:20485439]"
/experiment="EXISTENCE:direct assay:GO:0005096 GTPase
activator activity [PMID:9308967]"
/experiment="EXISTENCE:direct assay:GO:0006446 regulation
of translational initiation [PMID:22851688]"
/experiment="EXISTENCE:direct assay:GO:0031369 translation
initiation factor binding
[PMID:16380131|PMID:22683627|PMID:15145951]"
/experiment="EXISTENCE:direct assay:GO:0033290 eukaryotic
48S preinitiation complex [PMID:17242201]"
/experiment="EXISTENCE:direct assay:GO:0043614 multi-eIF
complex [PMID:11018020|PMID:15838098]"
/experiment="EXISTENCE:genetic interaction:GO:0045947
negative regulation of translational initiation
[PMID:20485439]"
/experiment="EXISTENCE:mutant phenotype:GO:0005092
GDP-dissociation inhibitor activity [PMID:20485439]"
/experiment="EXISTENCE:mutant phenotype:GO:0005096 GTPase
activator activity [PMID:9308967]"
/experiment="EXISTENCE:mutant phenotype:GO:0006446
regulation of translational initiation
[PMID:22851688|PMID:11331597]"
/experiment="EXISTENCE:mutant phenotype:GO:0042256
cytosolic ribosome assembly [PMID:11331597]"
/experiment="EXISTENCE:mutant phenotype:GO:0045947
negative regulation of translational initiation
[PMID:20485439]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:9395514|PMID:22683627]"
/experiment="EXISTENCE:physical interaction:GO:0031369
translation initiation factor binding
[PMID:22851688|PMID:15145951]"
/note="Translation initiation factor eIF5; functions as
both a GTPase-activating protein (GAP) that stimulates the
hydrolysis of GTP bound to eIF-2, as part of the 43S
preinitiation complex, and as a GDP dissociation inhibitor
(GDI) to prevent recycling of eIF2; human ortholog eIF5
complements the yeast null mutant"
/codon_start=1
/product="translation initiation factor eIF5"
/protein_id="NP_015366.1"
/db_xref="GeneID:856154"
/db_xref="SGD:S000006245"
/translation="MSINICRDNHDPFYRYKMPPIQAKVEGRGNGIKTAVLNVADISH
ALNRPAPYIVKYFGFELGAQTSISVDKDRYLVNGVHEPAKLQDVLDGFINKFVLCGSC
KNPETEIIITKDNDLVRDCKACGKRTPMDLRHKLSSFILKNPPDSVSGSKKKKKAATA
SANVRGGGLSISDIAQGKSQNAPSDGTGSSTPQHHDEDEDELSRQIKAAASTLEDIEV
KDDEWAVDMSEEAIRARAKELEVNSELTQLDEYGEWILEQAGEDKENLPSDVELYKKA
AELDVLNDPKIGCVLAQCLFDEDIVNEIAEHNAFFTKILVTPEYEKNFMGGIERFLGL
EHKDLIPLLPKILVQLYNNDIISEEEIMRFGTKSSKKFVPKEVSKKVRRAAKPFITWL
ETAESDDDEEDDE"
gene complement(<650435..>653662)
/gene="PUF2"
/locus_tag="YPR042C"
/db_xref="GeneID:856155"
mRNA complement(<650435..>653662)
/gene="PUF2"
/locus_tag="YPR042C"
/product="Puf2p"
/transcript_id="NM_001184139.1"
/db_xref="GeneID:856155"
CDS complement(650435..653662)
/gene="PUF2"
/locus_tag="YPR042C"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640|PMID:15024427|PMID:21685478]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0000288
nuclear-transcribed mRNA catabolic process,
deadenylation-dependent decay [PMID:11101532]"
/note="PUF family mRNA-binding protein; Pumilio homology
domain confers RNA binding activity; preferentially binds
mRNAs encoding membrane-associated proteins; binding site
composed of two UAAU tetranucleotides, separated by a 3-nt
linker; PUF2 has a paralog, JSN1, that arose from the
whole genome duplication"
/codon_start=1
/product="Puf2p"
/protein_id="NP_015367.1"
/db_xref="GeneID:856155"
/db_xref="SGD:S000006246"
/translation="MDNKRLYNGNLSNIPEVIDPGITIPIYEEDIRNDTRMNTNARSV
RVSDKRGRSSSTSPQKIGSYRTRAGRFSDTLTNLLPSISAKLHHSKKSTPVVVVPPTS
STPDSLNSTTYAPRVSSDSFTVATPLSLQSTTTRTRTRNNTVSSQITASSSLTTDVGN
ATSANIWSANAESNTSSSPLFDYPLATSYFEPLTRFKSTDNYTLPQTAQLNSFLEKNG
NPNIWSSAGNSNTDHLNTPIVNRQRSQSQSTTNRVYTDAPYYQQPAQNYQVQVPPRVP
KSTSISPVILDDVDPASINWITANQKVPLVNQISALLPTNTISISNVFPLQPTQQHQQ
NAVNLTSTSLATLCSQYGKVLSARTLRGLNMALVEFSTVESAICALEALQGKELSKVG
APSTVSFARVLPMYEQPLNVNGFNNTPKQPLLQEQLNHGVLNYQLQQSLQQPELQQQP
TSFNQPNLTYCNPTQNLSHLQLSSNENEPYPFPLPPPSLSDSKKDILHTISSFKLEYD
HLELNHLLQNALKNKGVSDTNYFGPLPEHNSKVPKRKDTFDAPKLRELRKQFDSNSLS
TIEMEQLAIVMLDQLPELSSDYLGNTVIQKLFENSSNIIRDIMLRKCNKYLTSMGVHK
NGTWVCQKIIKMANTPRQINLVTSGVSDYCTPLFNDQFGNYVIQGILKFGFPWNSFIF
ESVLSHFWTIVQNRYGSRAVRACLEADSIITQCQLLTITSLIIVLSPYLATDTNGTLL
ITWLLDTCTLPNKNLILCDKLVNKNLVKLCCHKLGSLTVLKILNLRGGEEEALSKNKI
IHAIFDGPISSDSILFQILDEGNYGPTFIYKVLTSRILDNSVRDEAITKIRQLILNSN
INLQSRQLLEEVGLSSAGISPKQSSKNHRKQHPQGFHSPGRARGVSVSSVRSSNSRHN
SVIQMNNAGPTPALNFNPAPMSEINSYFNNQQVVYSGNQNQNQNGNSNGLDELNSQFD
SFRIANGTNLSLPIVNLPNVSNNNNNYNNSGYSSQMNPLSRSVSHNNNNNTNNYNNND
NDNNNNNNNNNNNNNNNNNNNNNSNNSNNNNNNDTSLYRYRSYGY"
gene <654166..>654847
/gene="RPL43A"
/locus_tag="YPR043W"
/db_xref="GeneID:856156"
mRNA join(<654166..654167,654571..>654847)
/gene="RPL43A"
/locus_tag="YPR043W"
/product="60S ribosomal protein eL43 RPL43A"
/transcript_id="NM_001184140.1"
/db_xref="GeneID:856156"
CDS join(654166..654167,654571..654847)
/gene="RPL43A"
/locus_tag="YPR043W"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:11983894]"
/experiment="EXISTENCE:curator inference:GO:0003735
structural constituent of ribosome [PMID:11983894]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:11983894]"
/note="Ribosomal 60S subunit protein L43A; null mutation
confers a dominant lethal phenotype; homologous to
mammalian ribosomal protein L37A, no bacterial homolog;
RPL43A has a paralog, RPL43B, that arose from the whole
genome duplication"
/codon_start=1
/product="60S ribosomal protein eL43 RPL43A"
/protein_id="NP_015368.1"
/db_xref="GeneID:856156"
/db_xref="SGD:S000006247"
/translation="MAKRTKKVGITGKYGVRYGSSLRRQVKKLEIQQHARYDCSFCGK
KTVKRGAAGIWTCSCCKKTVAGGAYTVSTAAAATVRSTIRRLREMVEA"
gene complement(<655140..>656552)
/gene="THP3"
/locus_tag="YPR045C"
/db_xref="GeneID:856158"
mRNA complement(<655140..>656552)
/gene="THP3"
/locus_tag="YPR045C"
/product="Thp3p"
/transcript_id="NM_001184142.1"
/db_xref="GeneID:856158"
CDS complement(655140..656552)
/gene="THP3"
/locus_tag="YPR045C"
/experiment="EXISTENCE:direct assay:GO:0000791 euchromatin
[PMID:21149575]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0000398 mRNA
splicing, via spliceosome [PMID:19061648]"
/note="Protein that may have a role in transcription
elongation; forms a complex with Csn12p that is recruited
to transcribed genes; possibly involved in splicing based
on pre-mRNA accumulation defect for many intron-containing
genes"
/codon_start=1
/product="Thp3p"
/protein_id="NP_015370.1"
/db_xref="GeneID:856158"
/db_xref="SGD:S000006249"
/translation="MQNPYGHFTNNTTEDREASSQGGPFGQSLNRPLDYAGSFPSLTY
NNNNFIANQQPSLPLPEPRLSWNNVNQVSNPLMVTPLPGLQKRMNKNIKKKLPRVSKK
ASALSNGVSGNVMSNSNIVGHGAVGSASGWKVEMGGSDELERRKRRAERFSQGPSATT
NSNDNLNEDFANLNAISSKSHQYDKKIHVVGRCQTLEKSYLRLTSEPNPDLIRPPNIL
QKMYCLLMDKYQSKTATYTYLCDQFKSMRQDLRVQMIENSFTIKVYQTHARIALENGD
LGEFNQCQNRIMALFENPTIPKKSYSEFICYSVLYSMLTEDYPSISHLKLKLIDDGSS
EILEDEHVKMIFELSDMKLVGNYHYFMKNYLKLHKFEKCLINSFLNLEKLIFLTIICK
SYNQVNLDFVKSEFNFNSIEETTNFLNEQNLTEFILNKQITDSNGKSSNIKILNTKGC
RVQLIQNYMKSKKIDIKGQK"
gene <656799..>657344
/gene="MCM16"
/locus_tag="YPR046W"
/db_xref="GeneID:856159"
mRNA <656799..>657344
/gene="MCM16"
/locus_tag="YPR046W"
/product="Mcm16p"
/transcript_id="NM_001184143.1"
/db_xref="GeneID:856159"
CDS 656799..657344
/gene="MCM16"
/locus_tag="YPR046W"
/experiment="EXISTENCE:direct assay:GO:0000776 kinetochore
[PMID:11782448]"
/experiment="EXISTENCE:mutant phenotype:GO:0007059
chromosome segregation [PMID:11782448]"
/experiment="EXISTENCE:mutant phenotype:GO:0034087
establishment of mitotic sister chromatid cohesion
[PMID:19730685]"
/note="Component of the Ctf19 complex and the COMA
subcomplex; involved in kinetochore-microtubule mediated
chromosome segregation; binds to centromere DNA;
orthologous to human centromere constitutive-associated
network (CCAN) subunit CENP-H and fission yeast fta3"
/codon_start=1
/product="Mcm16p"
/protein_id="NP_015371.1"
/db_xref="GeneID:856159"
/db_xref="SGD:S000006250"
/translation="MTNSSEKQWERIQQLEKEHVEVYRELLITLDRLYLIRKHNHAVI
LSHTQQRLLEIRHQLQINLEKTALLIRLLEKPDNTNVLFTKLQNLLEESNSLDYELLQ
SLGAQSSLHKQLIESRAERDELMSKLIELSSKFPKPTIPPDDSDTAGKQVEVEKENET
IQELMIALQIHSGYTNISYTI"
gene <657529..>658938
/gene="MSF1"
/locus_tag="YPR047W"
/db_xref="GeneID:856160"
mRNA <657529..>658938
/gene="MSF1"
/locus_tag="YPR047W"
/product="phenylalanine--tRNA ligase"
/transcript_id="NM_001184144.1"
/db_xref="GeneID:856160"
CDS 657529..658938
/gene="MSF1"
/locus_tag="YPR047W"
/EC_number="6.1.1.20"
/experiment="EXISTENCE:direct assay:GO:0004826
phenylalanine-tRNA ligase activity [PMID:16162501]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:mutant phenotype:GO:0004826
phenylalanine-tRNA ligase activity [PMID:3029120]"
/experiment="EXISTENCE:mutant phenotype:GO:0005739
mitochondrion [PMID:3029120]"
/experiment="EXISTENCE:mutant phenotype:GO:0070156
mitochondrial phenylalanyl-tRNA aminoacylation
[PMID:3029120]"
/note="Mitochondrial phenylalanyl-tRNA synthetase; active
as a monomer, unlike the cytoplasmic subunit which is
active as a dimer complexed to a beta subunit dimer;
similar to the alpha subunit of E. coli phenylalanyl-tRNA
synthetase"
/codon_start=1
/product="phenylalanine--tRNA ligase"
/protein_id="NP_015372.2"
/db_xref="GeneID:856160"
/db_xref="SGD:S000006251"
/translation="MFLNRMMKTRTGLYRLYSTLKVPHVEINGIKYKTDPQTTNVTDS
IIKLTDRSLHLKESHPVGILRDLIEKKLNSVDNTFKIFNNFKPVVTTMENFDSLGFPK
DHPGRSKSDTYYINETHLLRTHTSAHELECFQKIRNDSDNIKSGFLISADVYRRDEID
KTHYPVFHQMEGATIWKRTKADVGVKEPMYIEKIREDIRQVENLLNKENVKITVDDDT
IPLKENNPKQEYMSDLEVDLCSQHLKRSIELIVSEVFNKKISSMIKNKANNTPKELKV
RWINAYFPWTAPSWEIEVWWQGEWLELCGCGLIRQDVLLRAGYKPSETIGWAFGLGLD
RIAMLLFEIPDIRLLWSRDERFSRQFSKGLITSFKPYSKHPGSFRDVAFWLPEDKPDI
HQVHENDLMEIIRNIAGDLVESVKLVDSFTHPKTGRKSMCYRINYQSMDRNLTNAEVN
TLQDMVCSKLVKEYSVELR"
gene <659182..>661053
/gene="TAH18"
/locus_tag="YPR048W"
/db_xref="GeneID:856161"
mRNA <659182..>661053
/gene="TAH18"
/locus_tag="YPR048W"
/product="NAPDH-dependent diflavin reductase"
/transcript_id="NM_001184145.1"
/db_xref="GeneID:856161"
CDS 659182..661053
/gene="TAH18"
/locus_tag="YPR048W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:19194512]"
/experiment="EXISTENCE:direct assay:GO:0160246
NADPH-iron-sulfur [2Fe-2S] protein oxidoreductase activity
[PMID:20802492]"
/experiment="EXISTENCE:mutant phenotype:GO:0006809 nitric
oxide biosynthetic process [PMID:23159617]"
/experiment="EXISTENCE:mutant phenotype:GO:0016226
iron-sulfur cluster assembly [PMID:20802492]"
/experiment="EXISTENCE:mutant phenotype:GO:0045429
positive regulation of nitric oxide biosynthetic process
[PMID:27178802]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:27178802]"
/note="Conserved NAPDH-dependent diflavin reductase;
component of an early step in the cytosolic Fe-S protein
assembly (CIA) machinery; transfers electrons from NADPH
to the Fe-S cluster of Dre2p; plays a pro-death role under
oxidative stress; Tah18p-dependent nitric oxide synthesis
confers high-temperature stress tolerance; possible target
for development of antifungal drugs"
/codon_start=1
/product="NAPDH-dependent diflavin reductase"
/protein_id="NP_015373.1"
/db_xref="GeneID:856161"
/db_xref="SGD:S000006252"
/translation="MSSSKKIVILYGSETGNAHDFATILSHRLHRWHFSHTFCSIGDY
DPQDILKCRYLFIICSTTGQGELPRNVNALKGERPVTFWSFLKRKNLPSNLLNHIQTA
MLGLGDSSYPKFNYGIRKLHQRIVTQLGANELFDRLEADDQAMAGSNKGTGLGIESVY
FEYEKKVLSFLLSKYPNRKVNGQIIKREELDPEVYLEPASYLQLSDEHANEKFTSTKV
IFEGDESLKVGRVNINKRITSEGHFQDVRQFKFSNVDKIQENYEPGDTVTIYPCNTDE
DVSRFLANQSHWLEIADKPLNFTSGVPNDLKDGGLVRPMTLRNLLKYHCDFMSIPRTS
FFLKIWTFATDVTKMERGQEQLNDQREKLRQFATDQDMQDLYDYCNRPRRSILEVLED
FISVKLPWKYVLDYLPIIKPRYYSISSGPGDPNIELTVAIVKYKTILRKIRRGICTNY
IARLQEGEQIRYKLQNNHIIKKEFLNKPMILVGPGVGLAPLLSVVKAEISKDIKLLFG
CRYKDKDYIYKDMLEDWFRKGKIALHSSFSRDEENSPGVKYVQDYLWRLGEEITNLVV
NKDAVFFLCGSSGKMPIQVRLTFIEMLKKWGNFSDEETAKKYLKEMEKSDRYIQETW"
gene complement(<661137..>664673)
/gene="ATG11"
/locus_tag="YPR049C"
/gene_synonym="CVT3; CVT9"
/db_xref="GeneID:856162"
mRNA complement(<661137..>664673)
/gene="ATG11"
/locus_tag="YPR049C"
/gene_synonym="CVT3; CVT9"
/product="autophagy protein ATG11"
/transcript_id="NM_001184146.1"
/db_xref="GeneID:856162"
CDS complement(661137..664673)
/gene="ATG11"
/locus_tag="YPR049C"
/gene_synonym="CVT3; CVT9"
/experiment="EXISTENCE:direct assay:GO:0000045
autophagosome assembly [PMID:18725539]"
/experiment="EXISTENCE:direct assay:GO:0000149 SNARE
binding [PMID:26774783]"
/experiment="EXISTENCE:direct assay:GO:0000407 phagophore
assembly site [PMID:15659643]"
/experiment="EXISTENCE:direct assay:GO:0005774 vacuolar
membrane [PMID:40173187]"
/experiment="EXISTENCE:direct assay:GO:0006914 autophagy
[PMID:28013049]"
/experiment="EXISTENCE:direct assay:GO:0006995 cellular
response to nitrogen starvation
[PMID:18725539|PMID:26774783]"
/experiment="EXISTENCE:direct assay:GO:0034497 protein
localization to phagophore assembly site
[PMID:26774783|PMID:23858448]"
/experiment="EXISTENCE:direct assay:GO:0071957 old mitotic
spindle pole body [PMID:40173187]"
/experiment="EXISTENCE:genetic interaction:GO:0032258
cytoplasm to vacuole targeting by the Cvt pathway
[PMID:15659643]"
/experiment="EXISTENCE:genetic interaction:GO:0060090
molecular adaptor activity [PMID:17295840]"
/experiment="EXISTENCE:genetic interaction:GO:2000786
positive regulation of autophagosome assembly
[PMID:18077553]"
/experiment="EXISTENCE:mutant phenotype:GO:0000422
autophagy of mitochondrion [PMID:19793921]"
/experiment="EXISTENCE:mutant phenotype:GO:0000425
pexophagy [PMID:11309418]"
/experiment="EXISTENCE:mutant phenotype:GO:0007059
chromosome segregation [PMID:40173187]"
/experiment="EXISTENCE:mutant phenotype:GO:0031503
protein-containing complex localization [PMID:12479808]"
/experiment="EXISTENCE:mutant phenotype:GO:0032258
cytoplasm to vacuole targeting by the Cvt pathway
[PMID:15659643]"
/experiment="EXISTENCE:mutant phenotype:GO:0034517
ribophagy [PMID:27659307]"
/experiment="EXISTENCE:mutant phenotype:GO:0034727
piecemeal microautophagy of the nucleus [PMID:18701704]"
/experiment="EXISTENCE:mutant phenotype:GO:0061709
reticulophagy [PMID:26181331]"
/note="Adapter protein for pexophagy and the Cvt targeting
pathway; directs receptor-bound cargo to the phagophore
assembly site (PAS) for packaging into vesicles; required
for the recruitment of Atg proteins to the PAS,
facilitating Cvt vesicle formation; recruits Dnm1p to
facilitate fission of mitochondria that are destined for
removal by mitophagy; dynamic association with the
daughter (old) SPB is involved in microtubule-mediated
chromosome segregation"
/codon_start=1
/product="autophagy protein ATG11"
/protein_id="NP_015374.1"
/db_xref="GeneID:856162"
/db_xref="SGD:S000006253"
/translation="MADADEYSTAPTQQEITPLQTTATIINAISGECITTNVDFFVSL
DKFKQFIARKWKIPPDQLLILLPYGNKLKPSMFKELLINRSFTLNDFYVYDRRLFSLV
SKPTPTNLLTSKDSNPMNSPNSNDLTETLEYLIKNSHISQYQGSDTIMIKPMPSPLED
ADVDLSRLNYHSVTSLLTTNLGWLSALEIDVHYFKSLIPDIIAHIKRIFDGLTVCSQY
LKLYCFDVESLYNSNVQFLNQLVDNGMTSKWEKCFNDTLSKLTALEGDSLQKFINIES
LLENEKSVKILNHSINGKLNKIKREIDENASFRDIITVNIDRLRQMFTPNESKFELED
QMAESFEVLVSEMRTRSRNVLDKEEEEFNSQEFLKSMNVMLEKDKKESVKTLFTISQA
LYSQIGELIDLKKSLQKHAVAILGNIAFTQMEILGIKRLLLNECNKDLELYKKYEVEF
AQVEDLPLIYGLYLIEKYRRLSWFQQILSFISNFNQDLELFKQNELRTRNKWVKNFGS
IATVFCEDLLSSSDFKRLNEYHSHTSPPNEDEEDENENSIANYRQDLVKVSQAIDNYM
TQIKETDVSEPIIDLLSKTLFETKRFHIIYSNFKNNNNNSSNGNSISPEGSIALKSDD
VVKGYKTRIKKLESLLHEFQYSDIGHWPQGVLNTHLKPFRGSATSINKKKFLGASVLL
EPANISEVNIDSVSQANNHQIQELESNVDDLLHQLQLLKEENNRKSMQISEMGKKISD
LEVEKTAYRETLTNLNQELARLTNEEQSHRTEIFTLNASFKKQLNDIISQDNEKIEKL
TGDYDDVSKSRERLQMDLDESNKKHEQEVNLLKADIERLGKQIVTSEKSYAETNSSSM
EKGEKFETIPLAEDPGRENQISAYTQTLQDRIFDIISTNIFILENIGLLLTFDNNNNI
QIRRVKGLKKGTAQSNILDESTQMLDAHDNSLIKSPVFQKLKDEYELIKSVANGSEKD
TQQSIFLGNITQLYDNKLYEVAVIRRFKDIETLAKKLTKENKIKRTLLERFQREKVTL
RNFQIGDLALFLPTRENVNSVGSMSSSTSSLSSSFSSVDLSTPPPLDAMSIQSSPSVI
HSNVINQASISGRDKNKLMRPWAAFTAFEESTRYFLKDEKGLTKGKEWFVGRIVTLEH
FVADSPSNNPFRLPKGSVWFQVTAVVVSYQGV"
gene <664960..>665490
/gene="MAK3"
/locus_tag="YPR051W"
/gene_synonym="NAA30"
/db_xref="GeneID:856163"
mRNA <664960..>665490
/gene="MAK3"
/locus_tag="YPR051W"
/gene_synonym="NAA30"
/product="peptide alpha-N-acetyltransferase MAK3"
/transcript_id="NM_001184148.1"
/db_xref="GeneID:856163"
CDS 664960..665490
/gene="MAK3"
/locus_tag="YPR051W"
/gene_synonym="NAA30"
/EC_number="2.3.1.256"
/experiment="EXISTENCE:direct assay:GO:0031417 NatC
complex [PMID:10504710]"
/experiment="EXISTENCE:mutant phenotype:GO:0004596
protein-N-terminal amino-acid acetyltransferase activity
[PMID:11274203]"
/experiment="EXISTENCE:mutant phenotype:GO:0006474
N-terminal protein amino acid acetylation [PMID:11274203]"
/experiment="EXISTENCE:mutant phenotype:GO:0032880
regulation of protein localization
[PMID:15077114|PMID:15077113|PMID:15911569|PMID:17261844]"
/note="Catalytic subunit of the NatC type N-terminal
acetyltransferase (NAT); involved in subcellular targeting
of select N-terminally acetylated substrates to the Golgi
apparatus (Arl3p and Grh1p) and the inner nuclear membrane
(Trm1p); required for replication of dsRNA virus; human
NatC ortholog, NAA30, functionally complements the null,
requiring either auxiliary subunit Mak10p or co-expression
of human ortholog, NAA35; NAA60, the human NatF gene, also
complements the null allele"
/codon_start=1
/product="peptide alpha-N-acetyltransferase MAK3"
/protein_id="NP_015376.1"
/db_xref="GeneID:856163"
/db_xref="SGD:S000006255"
/translation="MEIVYKPLDIRNEEQFASIKKLIDADLSEPYSIYVYRYFLNQWP
ELTYIAVDNKSGTPNIPIGCIVCKMDPHRNVRLRGYIGMLAVESTYRGHGIAKKLVEI
AIDKMQREHCDEIMLETEVENSAALNLYEGMGFIRMKRMFRYYLNEGDAFKLILPLTE
KSCTRSTFLMHGRLAT"
gene complement(<665693..>665974)
/gene="NHP6A"
/locus_tag="YPR052C"
/db_xref="GeneID:856165"
mRNA complement(<665693..>665974)
/gene="NHP6A"
/locus_tag="YPR052C"
/product="high-mobility group nucleosome-binding protein"
/transcript_id="NM_001184149.1"
/db_xref="GeneID:856165"
CDS complement(665693..665974)
/gene="NHP6A"
/locus_tag="YPR052C"
/experiment="EXISTENCE:direct assay:GO:0001195 maintenance
of transcriptional fidelity during transcription
elongation by RNA polymerase III [PMID:16407207]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:3169249|PMID:17878171]"
/experiment="EXISTENCE:direct assay:GO:0006325 chromatin
organization [PMID:19683499]"
/experiment="EXISTENCE:direct assay:GO:0008301 DNA
binding, bending [PMID:7721780|PMID:9468494]"
/experiment="EXISTENCE:direct assay:GO:0031491 nucleosome
binding [PMID:11432837|PMID:12952948|PMID:12805231]"
/experiment="EXISTENCE:direct assay:GO:0032407 MutSalpha
complex binding [PMID:19843605]"
/experiment="EXISTENCE:direct assay:GO:0043392 negative
regulation of DNA binding [PMID:19843605]"
/experiment="EXISTENCE:direct assay:GO:0051123 RNA
polymerase II preinitiation complex assembly
[PMID:8946917]"
/experiment="EXISTENCE:direct assay:GO:0070898 RNA
polymerase III preinitiation complex assembly
[PMID:17178828|PMID:16407207|PMID:11239460]"
/experiment="EXISTENCE:genetic interaction:GO:0006338
chromatin remodeling [PMID:14739928]"
/experiment="EXISTENCE:genetic interaction:GO:0070898 RNA
polymerase III preinitiation complex assembly
[PMID:11533232]"
/note="High-mobility group (HMG) protein; binds to and
remodels nucleosomes; involved in recruiting FACT and
other chromatin remodelling complexes to chromosomes;
functionally redundant with Nhp6Bp; required for
transcriptional initiation fidelity of some tRNA genes;
homologous to mammalian HMGB1 and HMGB2; NHP6A has a
paralog, NHP6B, that arose from the whole genome
duplication; protein abundance increases in response to
DNA replication stress"
/codon_start=1
/product="high-mobility group nucleosome-binding protein"
/protein_id="NP_015377.1"
/db_xref="GeneID:856165"
/db_xref="SGD:S000006256"
/translation="MVTPREPKKRTTRKKKDPNAPKRALSAYMFFANENRDIVRSENP
DITFGQVGKKLGEKWKALTPEEKQPYEAKAQADKKRYESEKELYNATLA"
gene complement(<665788..>666243)
/locus_tag="YPR053C"
/db_xref="GeneID:856166"
mRNA complement(<665788..>666243)
/locus_tag="YPR053C"
/product="uncharacterized protein"
/transcript_id="NM_001348887.1"
/db_xref="GeneID:856166"
CDS complement(665788..666243)
/locus_tag="YPR053C"
/note="hypothetical protein; conserved among S. cerevisiae
strains; YPR053C is not an essential gene; partially
overlaps verified ORF NHP6A/YPR052C"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335825.1"
/db_xref="GeneID:856166"
/db_xref="SGD:S000006257"
/translation="MMYRTTLNTVQVSQISGAEFYPHASSRAILFESPAFCRLFFSPF
VYLAVGKQTTQYLLLVPTVKEGLFWDVFFSCFCSIDYPIHSKAQSQWSPQENLRREPL
ERRRTQMPLRGLCPPTCFSLTKTEILFVLKIQISHLDKSARSWVRSGRL"
gene <666280..>667446
/gene="SMK1"
/locus_tag="YPR054W"
/db_xref="GeneID:856167"
mRNA <666280..>667446
/gene="SMK1"
/locus_tag="YPR054W"
/product="mitogen-activated protein kinase SMK1"
/transcript_id="NM_001184151.1"
/db_xref="GeneID:856167"
CDS 666280..667446
/gene="SMK1"
/locus_tag="YPR054W"
/EC_number="2.7.11.24"
/experiment="EXISTENCE:direct assay:GO:0004707 MAP kinase
activity [PMID:16116083]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0070056 prospore
membrane leading edge [PMID:33466572]"
/experiment="EXISTENCE:genetic interaction:GO:0030476
ascospore wall assembly [PMID:16116083]"
/experiment="EXISTENCE:mutant phenotype:GO:0004707 MAP
kinase activity [PMID:16116083]"
/experiment="EXISTENCE:mutant phenotype:GO:0030476
ascospore wall assembly [PMID:7958885|PMID:16116083]"
/experiment="EXISTENCE:mutant phenotype:GO:1904853 protein
localization to ascospore wall [PMID:38874213]"
/note="Middle sporulation-specific mitogen-activated
protein kinase (MAPK); required for prospore membrane
development and the production of outer spore wall layers;
promotes deposition of the glucan layer of the spore wall
by regulating the localization of Gsc2p to sites of spore
wall assembly in postmeiotic cells; activated by
Cak1p-mediated phosphorylation of threonine (T207) in the
activation loop during meiosis I followed by
Ssp2p-promoted autophosphorylation of tyrosine (T209)
during meiosis II"
/codon_start=1
/product="mitogen-activated protein kinase SMK1"
/protein_id="NP_015379.1"
/db_xref="GeneID:856167"
/db_xref="SGD:S000006258"
/translation="MNCTLTDNTRAINVASNLGAPQQRTIFAKERISIPGYYEIIQFL
GKGAYGTVCSVKFKGRSPAARIAVKKISNIFNKEILLKRAIRELKFMNFFKGHKNIVN
LIDLEIVTSSPYDGLYCYQELIDYDLAKVIHSSVQLSEFHIKYFLYQILCGLKYIHSA
DVIHRDLKPGNILCTLNGCLKICDFGLARGIHAGFFKCHSTVQPHITNYVATRWYRAP
ELLLSNQPYSKSVDIWAVGCILAEFYARKPVFMGRDSMHQIFEIIKVLGTPDKDILIK
FGTIKAWNLGKNSNNPVYKKIPWSNIFPFASHEAINLIESLLHWDSTHRLNVEQAISH
PFLNEVRKPDDEPVCLQGPFDFTYESELNSMSKLRDYLVEEVKNFKTDLSSSSL"
gene <667676..>670873
/gene="SEC8"
/locus_tag="YPR055W"
/db_xref="GeneID:856168"
mRNA <667676..>670873
/gene="SEC8"
/locus_tag="YPR055W"
/product="exocyst subunit SEC8"
/transcript_id="NM_001184152.1"
/db_xref="GeneID:856168"
CDS 667676..670873
/gene="SEC8"
/locus_tag="YPR055W"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:9491896]"
/experiment="EXISTENCE:direct assay:GO:0000145 exocyst
[PMID:7615633|PMID:1512289|PMID:8978675]"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip
[PMID:7615633|PMID:12960429|PMID:22842922|PMID:9491896|PMI
D:17717527]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:9491896|PMID:22842922|PMID:12960429]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807]"
/experiment="EXISTENCE:genetic interaction:GO:0006893
Golgi to plasma membrane transport [PMID:7026045]"
/experiment="EXISTENCE:mutant phenotype:GO:0006887
exocytosis [PMID:6996832]"
/experiment="EXISTENCE:mutant phenotype:GO:0006893 Golgi
to plasma membrane transport [PMID:7026045]"
/experiment="EXISTENCE:mutant phenotype:GO:0048309
endoplasmic reticulum inheritance [PMID:15596717]"
/experiment="EXISTENCE:mutant phenotype:GO:0048313 Golgi
inheritance [PMID:15596717]"
/note="Essential 121 kDa subunit of the exocyst complex;
the exocyst mediates polarized targeting and tethering of
post-Golgi secretory vesicles to active sites of
exocytosis at the plasma membrane prior to SNARE-mediated
fusion; involved in ER and Golgi inheritance in small
buds; relocalizes away from bud neck upon DNA replication
stress"
/codon_start=1
/product="exocyst subunit SEC8"
/protein_id="NP_015380.1"
/db_xref="GeneID:856168"
/db_xref="SGD:S000006259"
/translation="MDYLKPAQKGRRRGLSINSLSETQQSAMNSSLDHLQNDLNRINL
QWNRILSDNTNPLELALAFLDDTSVGLGHRYEEFNQLKSQIGSHLQDVVNEHSQVFNT
NVASYGKAVSSIMQAQEQTLNLKNCLKEANEKITTDKGSLQELNDNNLKYTKMIDVLV
NIEELLQIPEKIEENIRKENFHQVQILLERGFILMNNKSLKTVEILKPINQQLELQEH
LLFNNLIEEIHDIMYSKSNKTNFTRVTNNDIFKIISISHNGFTSLENYLYNIVNIDIM
EHSKTINKNLEQFIHDQSLNKGNIMLQENAATQAPLAPSRNQENEGFNRIGFLLKTIN
NINKLPVAFNIITERAKEEIHNIIVKSTESIRSKHPSLLKMATSLKNDNHFGLPVQDI
LSIILRECFWEIFLKLLYAIQCHRAIFEMSNILQPTSSAKPAFKFNKIWGKLLDEIEL
LLVRYINDPELISSNNGSIKPINGATNNAPTLPKRKNPKIFSLEYNIEDNSSVKDQAF
ELKALLKDIFPGFSVSSNMDLDSIYVKDESFEQDEPLVPPSVFNMKVILDPFLLFTQS
TSTIVPSVLTQNTISSLTFFDDYMNKSFLPKIQMTMDYLFTVEVESNNPYALELSDEN
HNIFKTALDFQRLFYNLLNVFNTANTFREKISYCILDLLNHFYNYYLGLFNSLIGTSD
RHLTRKIITAWLQNGILMDQEQKILNGDETLFHEESIELFKEIPHFYQAGKGLSKSDL
FNNLTLDTILQFSASVLWILNWLPGLKKAINIDEVSQEPMLDADRLRSSWTFSESMDL
NYSNPSSSPNSLGNLKILLDDKASKKFDETIDGFKTLKFKLITILRFNIRALCIYDIG
SFFQNTKIWNMDVGSIELDQNIASLISELRRTESKLKQQLPEKEKNSIFIGLDIVNNY
ALIKGAKSIKVLNHNGIKKMLRNVNVLQHAYRNLSSEPSKINMNVTMNFYSLCGSSEA
ELFEYIKDNELPHCSVEDLKTILRLQFSEEMHRQLKRQSTSSTKGSIKPSNKRYTEAL
EKLSNLEKEQSKEGARTKIGKLKSKLNAVHTANEK"
gene <671126..>672142
/gene="TFB4"
/locus_tag="YPR056W"
/db_xref="GeneID:856169"
mRNA <671126..>672142
/gene="TFB4"
/locus_tag="YPR056W"
/product="TFIIH/NER complex subunit TFB4"
/transcript_id="NM_001184153.1"
/db_xref="GeneID:856169"
CDS 671126..672142
/gene="TFB4"
/locus_tag="YPR056W"
/experiment="EXISTENCE:curator inference:GO:0016251 RNA
polymerase II general transcription initiation factor
activity [PMID:19818408]"
/experiment="EXISTENCE:direct assay:GO:0000439
transcription factor TFIIH core complex
[PMID:19818408|PMID:14500720]"
/experiment="EXISTENCE:direct assay:GO:0005675
transcription factor TFIIH holo complex
[PMID:19818408|PMID:27381459]"
/experiment="EXISTENCE:direct assay:GO:0006366
transcription by RNA polymerase II [PMID:19818408]"
/experiment="EXISTENCE:mutant phenotype:GO:0006289
nucleotide-excision repair [PMID:10506223]"
/experiment="EXISTENCE:physical interaction:GO:0000112
nucleotide-excision repair factor 3 complex
[PMID:10681587]"
/note="Subunit of TFIIH complex; involved in transcription
initiation, similar to 34 kDa subunit of human TFIIH;
interacts with Ssl1p"
/codon_start=1
/product="TFIIH/NER complex subunit TFB4"
/protein_id="NP_015381.1"
/db_xref="GeneID:856169"
/db_xref="SGD:S000006260"
/translation="MDAISDPTFKHARSRKQVTEESPSLLTVIIEIAPKLWTTFDEEG
NEKGSIIKVLEALIVFLNAHLAFNSANKVAVIAAYSQGIKYLYPESTSALKASESENK
TRSDLKIINSDMYRRFRNVDETLVEEIYKLFELEKKQIEQNSQRSTLAGAMSAGLTYV
NRISKESVTTSLKSRLLVLTCGSGSSKDEIFQYIPIMNCIFSATKMKCPIDVVKIGGS
KESTFLQQTTDATNGVYLHVESTEGLIQYLATAMFIDPSLRPIIVKPNHGSVDFRTSC
YLTGRVVAVGFICSVCLCVLSIIPPGNKCPACDSQFDEHVIAKLKRKPVVPRLKAKKK
VTKP"
gene <672471..>673496
/gene="BRR1"
/locus_tag="YPR057W"
/db_xref="GeneID:856170"
mRNA <672471..>673496
/gene="BRR1"
/locus_tag="YPR057W"
/product="Brr1p"
/transcript_id="NM_001184154.1"
/db_xref="GeneID:856170"
CDS 672471..673496
/gene="BRR1"
/locus_tag="YPR057W"
/experiment="EXISTENCE:direct assay:GO:0000387
spliceosomal snRNP assembly [PMID:40175367]"
/experiment="EXISTENCE:direct assay:GO:0017069 snRNA
binding [PMID:8861964]"
/experiment="EXISTENCE:direct assay:GO:0030532 small
nuclear ribonucleoprotein complex [PMID:8861964]"
/experiment="EXISTENCE:mutant phenotype:GO:0000245
spliceosomal complex assembly [PMID:8861964]"
/note="snRNP protein component of spliceosomal snRNPs;
required for pre-mRNA splicing and snRNP biogenesis; in
null mutant newly-synthesized snRNAs are destabilized and
3'-end processing is slowed"
/codon_start=1
/product="Brr1p"
/protein_id="NP_015382.1"
/db_xref="GeneID:856170"
/db_xref="SGD:S000006261"
/translation="MKRGESQAPDAIFGQSRAFALSDSSVNPDVIEYLKSVRQEALRT
NAISIKNHMNLQKRTRHKSSMYDDEDEGALKRHAISPSLIRLQRNVEIWVRWFNSVKA
TVLTNAYEFTGYEDETLDLLLLFLKNYLEDMPSKCTTVEKIISVLNQHSFPEKAEEKE
ENLQIDEEWAKNILVRLEKTKIDSVEDVKKVITEGDKHELVGYNQWFQYLINNEPQHT
TFHEKITSKQLWVLIKYMSNTWIKEIHKKGRHYRRLQDWLFYILVHTPERVTAEYTSI
LRDLGKKCLELIQKKPVEAHENKITLPKEMAELNVEIPAAVENMTITELTVSVIAVNY
GQKDLIE"
gene <673751..>674674
/gene="YMC1"
/locus_tag="YPR058W"
/db_xref="GeneID:856171"
mRNA <673751..>674674
/gene="YMC1"
/locus_tag="YPR058W"
/product="organic acid transporter"
/transcript_id="NM_001184155.1"
/db_xref="GeneID:856171"
CDS 673751..674674
/gene="YMC1"
/locus_tag="YPR058W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:16140254|PMID:24769239]"
/experiment="EXISTENCE:genetic interaction:GO:0006783 heme
biosynthetic process [PMID:26821380]"
/experiment="EXISTENCE:genetic interaction:GO:0006839
mitochondrial transport [PMID:16140254]"
/experiment="EXISTENCE:genetic interaction:GO:0015187
glycine transmembrane transporter activity
[PMID:26821380]"
/experiment="EXISTENCE:genetic interaction:GO:0022857
transmembrane transporter activity [PMID:16140254]"
/experiment="EXISTENCE:genetic interaction:GO:1904983
glycine import into mitochondrion [PMID:26821380]"
/note="Secondary mitochondrial inner membrane glycine
transporter; required with HEM25 for the transport of
glycine into mitochondria for the initiation of heme
biosynthesis; proposed role in oleate metabolism and
glutamate biosynthesis; member of the mitochondrial
carrier (MCF) family; localizes to the vacuole in response
to H2O2; YMC1 has a paralog, YMC2, that arose from the
whole genome duplication"
/codon_start=1
/product="organic acid transporter"
/protein_id="NP_015383.1"
/db_xref="GeneID:856171"
/db_xref="SGD:S000006262"
/translation="MSEEFPSPQLIDDLEEHPQHDNARVVKDLLAGTAGGIAQVLVGQ
PFDTTKVRLQTSSTPTTAMEVVRKLLANEGPRGFYKGTLTPLIGVGACVSLQFGVNEA
MKRFFHHRNADMSSTLSLPQYYACGVTGGIVNSFLASPIEHVRIRLQTQTGSGTNAEF
KGPLECIKKLRHNKALLRGLTPTILREGHGCGTYFLVYEALIANQMNKRRGLERKDIP
AWKLCIFGALSGTALWLMVYPLDVIKSVMQTDNLQKPKFGNSISSVAKTLYANGGIGA
FFKGFGPTMLRAAPANGATFATFELAMRLLG"
gene complement(<674861..>675631)
/gene="ARO7"
/locus_tag="YPR060C"
/gene_synonym="HGS1; OSM2; TYR7"
/db_xref="GeneID:856173"
mRNA complement(<674861..>675631)
/gene="ARO7"
/locus_tag="YPR060C"
/gene_synonym="HGS1; OSM2; TYR7"
/product="chorismate mutase ARO7"
/transcript_id="NM_001184157.1"
/db_xref="GeneID:856173"
CDS complement(674861..675631)
/gene="ARO7"
/locus_tag="YPR060C"
/gene_synonym="HGS1; OSM2; TYR7"
/EC_number="5.4.99.5"
/experiment="EXISTENCE:direct assay:GO:0004106 chorismate
mutase activity [PMID:3027508]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0006571
L-tyrosine biosynthetic process [PMID:3027508]"
/experiment="EXISTENCE:mutant phenotype:GO:0009094
L-phenylalanine biosynthetic process [PMID:3027508]"
/note="Chorismate mutase; catalyzes the conversion of
chorismate to prephenate to initiate the
tyrosine/phenylalanine-specific branch of aromatic amino
acid biosynthesis"
/codon_start=1
/product="chorismate mutase ARO7"
/protein_id="NP_015385.1"
/db_xref="GeneID:856173"
/db_xref="SGD:S000006264"
/translation="MDFTKPETVLNLQNIRDELVRMEDSIIFKFIERSHFATCPSVYE
ANHPGLEIPNFKGSFLDWALSNLEIAHSRIRRFESPDETPFFPDKIQKSFLPSINYPQ
ILAPYAPEVNYNDKIKKVYIEKIIPLISKRDGDDKNNFGSVATRDIECLQSLSRRIHF
GKFVAEAKFQSDIPLYTKLIKSKDVEGIMKNITNSAVEEKILERLTKKAEVYGVDPTN
ESGERRITPEYLVKIYKEIVIPITKEVEVEYLLRRLEE"
gene complement(<675977..>676882)
/gene="JID1"
/locus_tag="YPR061C"
/db_xref="GeneID:856174"
mRNA complement(<675977..>676882)
/gene="JID1"
/locus_tag="YPR061C"
/product="Jid1p"
/transcript_id="NM_001184158.1"
/db_xref="GeneID:856174"
CDS complement(675977..676882)
/gene="JID1"
/locus_tag="YPR061C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:17054397|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:19682992]"
/note="Probable Hsp40p co-chaperone; has a DnaJ-like
domain and appears to be involved in ER-associated
degradation of misfolded proteins containing a tightly
folded cytoplasmic domain; inhibits replication of Brome
mosaic virus in S. cerevisiae"
/codon_start=1
/product="Jid1p"
/protein_id="NP_015386.1"
/db_xref="GeneID:856174"
/db_xref="SGD:S000006265"
/translation="MLHHKFVYPFLFKWHLSCVEKCPPQITFIAKYATANDKNGNRKL
TIRDEQWPELADPTPYDIFGIPKAGSGNPKLDKKSLKKKYHRYVKLYHPDHSDNIQIF
SSEKVTNSDSKSPLLLTSSEKLHRFKVISQAYDILCDPKKKIVYDTTRQGWTTSYSPR
SNVNTENYQYAGSYGYHSNAQYEYWNAGTWEDANSMKNERIQENINPWTVIGIICGLA
ICIEGTALLAKIQESLSKAEFTHDESGLHLIQSYTNYGLDTDKFSRLRRFLWFRTWGL
YKSKEDLDREAKINEEMIRKLKAAK"
gene <677165..>677641
/gene="FCY1"
/locus_tag="YPR062W"
/db_xref="GeneID:856175"
mRNA <677165..>677641
/gene="FCY1"
/locus_tag="YPR062W"
/product="cytosine deaminase"
/transcript_id="NM_001184159.1"
/db_xref="GeneID:856175"
CDS 677165..677641
/gene="FCY1"
/locus_tag="YPR062W"
/EC_number="3.5.4.1"
/experiment="EXISTENCE:direct assay:GO:0004131 cytosine
deaminase activity [PMID:15535715|PMID:9000374]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0008655
pyrimidine-containing compound salvage [PMID:9000374]"
/experiment="EXISTENCE:mutant phenotype:GO:0019858
cytosine metabolic process [PMID:9000374]"
/experiment="EXISTENCE:mutant phenotype:GO:0046087
cytidine metabolic process [PMID:10501935]"
/note="Cytosine deaminase; zinc metalloenzyme that
catalyzes the hydrolytic deamination of cytosine to
uracil; of biomedical interest because it also catalyzes
the deamination of 5-fluorocytosine (5FC) to form
anticancer drug 5-fluorouracil (5FU)"
/codon_start=1
/product="cytosine deaminase"
/protein_id="NP_015387.1"
/db_xref="GeneID:856175"
/db_xref="SGD:S000006266"
/translation="MVTGGMASKWDQKGMDIAYEEAALGYKEGGVPIGGCLINNKDGS
VLGRGHNMRFQKGSATLHGEISTLENCGRLEGKVYKDTTLYTTLSPCDMCTGAIIMYG
IPRCVVGENVNFKSKGEKYLQTRGHEVVVVDDERCKKIMKQFIDERPQDWFEDIGE"
gene complement(<677812..>678320)
/locus_tag="YPR063C"
/db_xref="GeneID:856176"
mRNA complement(join(<677812..678193,678280..>678320))
/locus_tag="YPR063C"
/product="uncharacterized protein"
/transcript_id="NM_001184160.1"
/db_xref="GeneID:856176"
CDS complement(join(677812..678193,678280..678320))
/locus_tag="YPR063C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095]"
/note="ER-localized hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015388.1"
/db_xref="GeneID:856176"
/db_xref="SGD:S000006267"
/translation="MTLNNVARPDLCVSYKKIAPPKGLYSATPSISGVVNQSMPMAAI
FLRNKFIAWFSLIQSVHYYLNTDEDIIVAYKENKAPSPMDQPPAIKLFMSLIGLCVCY
MNLVFPQQIAQPSSSGSKGNTETTIETTTEVETETAKQ"
gene <678951..>679370
/locus_tag="YPR064W"
/db_xref="GeneID:856177"
mRNA <678951..>679370
/locus_tag="YPR064W"
/product="uncharacterized protein"
/transcript_id="NM_001348888.1"
/db_xref="GeneID:856177"
CDS 678951..679370
/locus_tag="YPR064W"
/note="hypothetical protein; conserved among S. cerevisiae
strains; YPR064W is not an essential gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335826.1"
/db_xref="GeneID:856177"
/db_xref="SGD:S000006268"
/translation="MKSCVLSNYVEGLEIIVCGYRNRLLFPFRATQVQAYFKVFSFFF
FLLLTLGAAAAAKPTSERQVIFGSADKSPGYHWPVEVSAAWNYRELLCKTIGNLVGKP
CSRTWRLLTKKKRAYCCCLFCCSSSYCLAGVLCVFCV"
gene <679693..>680799
/gene="ROX1"
/locus_tag="YPR065W"
/gene_synonym="REO1"
/db_xref="GeneID:856178"
mRNA <679693..>680799
/gene="ROX1"
/locus_tag="YPR065W"
/gene_synonym="REO1"
/product="Rox1p"
/transcript_id="NM_001184162.1"
/db_xref="GeneID:856178"
CDS 679693..680799
/gene="ROX1"
/locus_tag="YPR065W"
/gene_synonym="REO1"
/experiment="EXISTENCE:curator inference:GO:0000785
chromatin
[PMID:10487921|PMID:8413209|PMID:7565763|PMID:10446242]"
/experiment="EXISTENCE:direct assay:GO:0000987
cis-regulatory region sequence-specific DNA binding
[PMID:10487921]"
/experiment="EXISTENCE:direct assay:GO:0008301 DNA
binding, bending [PMID:7565763|PMID:10446242]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding
[PMID:8413209|PMID:10446242]"
/experiment="EXISTENCE:genetic interaction:GO:0071470
cellular response to osmotic stress [PMID:23435728]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:21299653|PMID:16835445|PMID:1588959|PMID:10446242|PM
ID:3062365|PMID:10487921|PMID:2847035|PMID:9388292|PMID:12
051835|PMID:12095998|PMID:2546055]"
/experiment="EXISTENCE:mutant phenotype:GO:0001227
DNA-binding transcription repressor activity, RNA
polymerase II-specific [PMID:10446242]"
/experiment="EXISTENCE:mutant phenotype:GO:0003714
transcription corepressor activity [PMID:14665463]"
/experiment="EXISTENCE:mutant phenotype:GO:0036295
cellular response to increased oxygen levels
[PMID:3062365|PMID:10487921|PMID:16835445|PMID:1588959|PMI
D:2546055|PMID:2847035|PMID:10446242|PMID:12051835|PMID:93
88292|PMID:12095998]"
/experiment="EXISTENCE:mutant phenotype:GO:0071470
cellular response to osmotic stress [PMID:23435728]"
/experiment="EXISTENCE:mutant phenotype:GO:0071472
cellular response to salt stress [PMID:21299653]"
/note="Heme-dependent repressor of hypoxic genes; mediates
aerobic transcriptional repression of hypoxia induced
genes such as COX5b and CYC7; repressor function regulated
through decreased promoter occupancy in response to
oxidative stress; contains an HMG domain that is
responsible for DNA bending activity; involved in the
hyperosmotic stress resistance"
/codon_start=1
/product="Rox1p"
/protein_id="NP_015390.1"
/db_xref="GeneID:856178"
/db_xref="SGD:S000006269"
/translation="MNPKSSTPKIPRPKNAFILFRQHYHRILIDEWTAQGVEIPHNSN
ISKIIGTKWKGLQPEDKAHWENLAEKEKLEHERKYPEYKYKPVRKSKKKQLLLKEIEQ
QQQQQQKEQQQQKQSQPQLQQPFNNNIVLMKRAHSLSPSSSVSSSNSYQFQLNNDLKR
LPIPSVNTSNYMVSRSLSGLPLTHDKTARDLPQLSSQLNSIPYYSAPHDPSTRHHYLN
VAQAQPRANSTPQLPFISSIINNSSQTPVTTTTTSTTTATSSPGKFSSSPNSSVLENN
RLNSINNSNQYLPPPLLPSLQDFQLDQYQQLKQMGPTYIVKPLSHTRNNLLSTTTPTH
HHIPHIPNQNIPLHQIINSSNTEVTAKTSLVSPK"
gene <681213..>682112
/gene="UBA3"
/locus_tag="YPR066W"
/db_xref="GeneID:856179"
mRNA <681213..>682112
/gene="UBA3"
/locus_tag="YPR066W"
/product="NEDD8-activating protein UBA3"
/transcript_id="NM_001184163.1"
/db_xref="GeneID:856179"
CDS 681213..682112
/gene="UBA3"
/locus_tag="YPR066W"
/EC_number="6.2.1.64"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0019781 NEDD8
activating enzyme activity [PMID:9545234]"
/experiment="EXISTENCE:direct assay:GO:0045116 protein
neddylation [PMID:9545234]"
/experiment="EXISTENCE:mutant phenotype:GO:0045116 protein
neddylation [PMID:9545234]"
/note="Protein that activates Rub1p (NEDD8) before
neddylation; acts together with Ula1p; may play a role in
protein degradation; GFP-fusion protein localizes to the
cytoplasm in a punctate pattern"
/codon_start=1
/product="NEDD8-activating protein UBA3"
/protein_id="NP_015391.1"
/db_xref="GeneID:856179"
/db_xref="SGD:S000006270"
/translation="MDCKILVLGAGGLGCEILKNLTMLSFVKQVHIVDIDTIELTNLN
RQFLFCDKDIGKPKAQVAAQYVNTRFPQLEVVAHVQDLTTLPPSFYKDFQFIISGLDA
IEPRRFINETLVKLTLESNYEICIPFIDGGTEGLKGHVKTIIPGITACWECSIDTLPS
QQDTVPMCTIANNPRCIEHVVEYVSTIQYPDLNIESTADMEFLLEKCCERAAQFSIST
EKLSTSFILGIIKSIIPSVSTTNAMVAATCCTQMVKIYNDLIDLENGNNFTLINCSEG
CFMYSFKFERLPDCTVCSNSNSN"
gene <682220..>682777
/gene="ISA2"
/locus_tag="YPR067W"
/db_xref="GeneID:856180"
mRNA <682220..>682777
/gene="ISA2"
/locus_tag="YPR067W"
/product="Isa2p"
/transcript_id="NM_001184164.1"
/db_xref="GeneID:856180"
CDS 682220..682777
/gene="ISA2"
/locus_tag="YPR067W"
/experiment="EXISTENCE:direct assay:GO:0005506 iron ion
binding [PMID:21987576]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005758
mitochondrial intermembrane space [PMID:10805735]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:10913600]"
/experiment="EXISTENCE:genetic interaction:GO:0009102
biotin biosynthetic process [PMID:17259550]"
/experiment="EXISTENCE:genetic interaction:GO:0016226
iron-sulfur cluster assembly [PMID:10805735]"
/experiment="EXISTENCE:mutant phenotype:GO:0009102 biotin
biosynthetic process [PMID:17259550]"
/experiment="EXISTENCE:mutant phenotype:GO:0016226
iron-sulfur cluster assembly
[PMID:10913600|PMID:10805735]"
/experiment="EXISTENCE:mutant phenotype:GO:0044572
[4Fe-4S] cluster assembly [PMID:21987576]"
/note="Protein required for maturation of mitochondrial
[4Fe-4S] proteins; functions in a complex with Isa1p and
possibly Iba57p; localizes to the mitochondrial
intermembrane space, overexpression of ISA2 suppresses
grx5 mutations"
/codon_start=1
/product="Isa2p"
/protein_id="NP_015392.1"
/db_xref="GeneID:856180"
/db_xref="SGD:S000006271"
/translation="MQAKLLFTRLNFRRPSTTLRQFPLTCFLFHSKAFYSDLVTKEPL
ITPKRIINKTPGLNLSISERASNRLAEIYRNSKENLRISVESGGCHGFQYNLTLEPAT
KPDIKNDVKDKEFSDDLDDDDSKDIIYVLPEDKGRVIIDSKSLNILNNTTLTYTNELI
GSSFKIINGSLKSSCGCGSSFDIEN"
gene complement(<682941..>684353)
/gene="HOS1"
/locus_tag="YPR068C"
/gene_synonym="CST3"
/db_xref="GeneID:856181"
mRNA complement(<682941..>684353)
/gene="HOS1"
/locus_tag="YPR068C"
/gene_synonym="CST3"
/product="histone deacetylase"
/transcript_id="NM_001184165.1"
/db_xref="GeneID:856181"
CDS complement(682941..684353)
/gene="HOS1"
/locus_tag="YPR068C"
/gene_synonym="CST3"
/EC_number="3.5.1.98"
/experiment="EXISTENCE:direct assay:GO:0033558 protein
lysine deacetylase activity [PMID:20797861|PMID:20832720]"
/experiment="EXISTENCE:mutant phenotype:GO:0033558 protein
lysine deacetylase activity [PMID:20797861|PMID:20832720]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:17706600]"
/experiment="EXISTENCE:physical interaction:GO:0000118
histone deacetylase complex [PMID:22543816]"
/note="Class I histone deacetylase (HDAC) family member;
deacetylates Smc3p on lysine residues at anaphase onset;
has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p;
interacts with the Tup1p-Ssn6p corepressor complex"
/codon_start=1
/product="histone deacetylase"
/protein_id="NP_015393.1"
/db_xref="GeneID:856181"
/db_xref="SGD:S000006272"
/translation="MSKLVISTSIFQSQVADLLPCNNHQKSQLTYSLINAYDLLQHFD
EVLTFPYARKDDLLEFHSKSYIDYLINGRFNKMMAQDVNNPMVESKWSELSELADNWN
EKIDYNPSQDLQRFTTRENLYNYYLNHSQALENNMDCINNSEVPTNDKPTDTYILNSE
TKQYNLEGDCPIFSYLPMYCQVITGATLNLLDHLSPTERLIGINWDGGRHHAFKQRAS
GFCYINDVVLLIQRLRKAKLNKITYVDFDLHHGDGVEKAFQYSKQIQTISVHLYEPGF
FPGTGSLSDSRKDKNVVNIPLKHGCDDNYLELIASKIVNPLIERHEPEALIIECGGDG
LLGDRFNEWQLTIRGLSRIIINIMKSYPRAHIFLLGGGGYNDLLMSRFYTYLTWCVTK
QFSNLRCGDNNSFQIDPFDVCDGDDSEQFIREHDLVEMYNEENYQYWIYEMEGSSRMK
MLRNDNKDRDMVELMKFYEL"
rep_origin 684389..684637
/note="ARS1624; Autonomously Replicating Sequence"
/db_xref="SGD:S000118420"
gene complement(<684556..>685437)
/gene="SPE3"
/locus_tag="YPR069C"
/db_xref="GeneID:856182"
mRNA complement(<684556..>685437)
/gene="SPE3"
/locus_tag="YPR069C"
/product="spermidine synthase"
/transcript_id="NM_001184166.1"
/db_xref="GeneID:856182"
CDS complement(684556..685437)
/gene="SPE3"
/locus_tag="YPR069C"
/EC_number="2.5.1.16"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0004766
spermidine synthase activity [PMID:9073064]"
/experiment="EXISTENCE:mutant phenotype:GO:0008295
spermidine biosynthetic process [PMID:9073064]"
/experiment="EXISTENCE:mutant phenotype:GO:0015940
pantothenate biosynthetic process [PMID:11154694]"
/note="Spermidine synthase; involved in biosynthesis of
spermidine and also in biosynthesis of pantothenic acid;
spermidine is required for growth of wild-type cells"
/codon_start=1
/product="spermidine synthase"
/protein_id="NP_015394.1"
/db_xref="GeneID:856182"
/db_xref="SGD:S000006273"
/translation="MAQEITHPTIVDGWFREISDTMWPGQAMTLKVEKVLHHEKSKYQ
DVLIFKSTTYGNVLVLDNVIQATERDEFAYQEMIAHLALNSHPNPKKVLVIGGGDGGV
LREVVKHDSVEEAWLCDIDEAVIRLSKEYLPEMAASYSHPKVKTHIGDGFQFLRDYQN
TFDVIITDSSDPEGPAETLFQKEYFQLLNSALTEKGVITTQAESMWIHLPIIKDLKKA
CSEVFPVAEYSFVTIPTYPTGTIGFMVCSKDKTCNVKKPLREISDEKEAELYRYYNKK
IHEASFVLPTWAAKELN"
gene <685898..>687598
/gene="MED1"
/locus_tag="YPR070W"
/db_xref="GeneID:856183"
mRNA <685898..>687598
/gene="MED1"
/locus_tag="YPR070W"
/product="Med1p"
/transcript_id="NM_001184167.1"
/db_xref="GeneID:856183"
CDS 685898..687598
/gene="MED1"
/locus_tag="YPR070W"
/experiment="EXISTENCE:direct assay:GO:0070847 core
mediator complex [PMID:9891034]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:9892641]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:9892641]"
/note="Subunit of the RNA polymerase II mediator complex;
associates with core polymerase subunits to form the RNA
polymerase II holoenzyme; essential for transcriptional
regulation"
/codon_start=1
/product="Med1p"
/protein_id="NP_015395.1"
/db_xref="GeneID:856183"
/db_xref="SGD:S000006274"
/translation="MVEGDSYVETLDSMIELFKDYKPGSITLENITRLCQTLGLESFT
EELSNELSRLSTASKIIVIDVDYNKKQDRIQDVKLVLASNFDNFDYFNQRDGEHEKSN
ILLNSLTKYPDLKAFHNNLKFLYLLDAYSHIESDSTSHNNGSSDKSLDSSNASFNNQG
KLDLFKYFTELSHYIRQCFQDNCCDFKVRTNLNDKFGIYILTQGINGKEVPLAKIYLE
ENKSDSQYRFYEYIYSQETKSWINESAENFSNGISLVMEIVANAKESNYTDLIWFPED
FISPELIIDKVTCSSNSSSSPPIIDLFSNNNYNSRIQLMNDFTTKLINIKKFDISNDN
LDLISEILKWVQWSRIVLQNVFKLVSTPSSNSNSSELEPDYQAPFSTSTKDKNSSTSN
TEPIPRSNRHGSVVEASRRRRSSTNKSKRPSITEAMMLKEEGLQQFNLHEILSEPAIE
EENGDSIKEHSTTMDGANDLGFTASVSNQENAGTDIVMEDHGVLQGTSQNYGTATADD
ADIEMKDVSSKPSKPESSVLQLIVSEDHIILDTISECNLYDDVKCWSKFIEKFQDIVS
"
gene <688172..>688807
/locus_tag="YPR071W"
/db_xref="GeneID:856184"
mRNA <688172..>688807
/locus_tag="YPR071W"
/product="uncharacterized protein"
/transcript_id="NM_001184168.1"
/db_xref="GeneID:856184"
CDS 688172..688807
/locus_tag="YPR071W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="Putative membrane protein; YPR071W is not an
essential gene; YPR071W has a paralog, YIL029C, that arose
from a single-locus duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015396.1"
/db_xref="GeneID:856184"
/db_xref="SGD:S000006275"
/translation="MQNGTEDKSNIPARSNDDVLPPLAVRLTMKVMRLIFIGKMFAYS
FVPFPPFKLLTFDNTVGWFVAYSAIVSIWGFAVWMERGYRHKINLLPPRCTKIRCSRC
NTRIRSPNWFKYKNWLYFFLLYVSLTTSNLIIQLASFMTEMSRRGISVPGTKDPGKRD
YLGLIIPMRFIGAFIHYMTANLFKEYYLHNGPLEKNDRPSTDEKTSENETL"
gene 689565..689646
/gene="SUP16"
/locus_tag="YNCP0012W"
/db_xref="GeneID:856185"
tRNA 689565..689646
/gene="SUP16"
/locus_tag="YNCP0012W"
/product="tRNA-Ser"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE
analysis; can mutate to suppress ochre nonsense mutations"
/db_xref="GeneID:856185"
/db_xref="SGD:S000006736"
gene <690107..>691789
/gene="NOT5"
/locus_tag="YPR072W"
/db_xref="GeneID:856186"
mRNA <690107..>691789
/gene="NOT5"
/locus_tag="YPR072W"
/product="CCR4-NOT core subunit NOT5"
/transcript_id="NM_001184169.1"
/db_xref="GeneID:856186"
CDS 690107..691789
/gene="NOT5"
/locus_tag="YPR072W"
/experiment="EXISTENCE:direct assay:GO:0000289
nuclear-transcribed mRNA poly(A) tail shortening
[PMID:11889048]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11889048]"
/experiment="EXISTENCE:direct assay:GO:0032968 positive
regulation of transcription elongation by RNA polymerase
II [PMID:21406554]"
/experiment="EXISTENCE:mutant phenotype:GO:0000290
deadenylation-dependent decapping of nuclear-transcribed
mRNA [PMID:26952104]"
/experiment="EXISTENCE:mutant phenotype:GO:0006368
transcription elongation by RNA polymerase II
[PMID:11404327]"
/experiment="EXISTENCE:mutant phenotype:GO:0016567 protein
ubiquitination [PMID:16926149]"
/experiment="EXISTENCE:mutant phenotype:GO:0031087
deadenylation-independent decapping of nuclear-transcribed
mRNA [PMID:15706350]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:26952104]"
/experiment="EXISTENCE:physical interaction:GO:0030015
CCR4-NOT core complex [PMID:10490603]"
/note="Component of the CCR4-NOT core complex, involved in
mRNA decapping; involved intranscription initiation and
elongation and in mRNA degradation; conserved lysine in
human homolog of Not5p and Not3p is mutated in cancers"
/codon_start=1
/product="CCR4-NOT core subunit NOT5"
/protein_id="NP_015397.1"
/db_xref="GeneID:856186"
/db_xref="SGD:S000006276"
/translation="MSQRKLQQDIDKLLKKVKEGIEDFDDIYEKFQSTDPSNSSHREK
LESDLKREIKKLQKHRDQIKTWLSKEDVKDKQSVLMTNRRLIENGMERFKSVEKLMKT
KQFSKEALTNPDIIKDPKELKKRDQVLFIHDCLDELQKQLEQYEAQENEEQTERHEFH
IANLENILKKLQNNEMDPEPVEEFQDDIKYYVENNDDPDFIEYDTIYEDMGCEIQPSS
SNNEAPKEGNNQTSLSSIRSSKKQERSPKKKAPQRDVSISDRATTPIAPGVESASQSI
SSTPTPVSTDTPLHTVKDDSIKFDNSTLGTPTTHVSMKKKESENDSEQQLNFPPDRTD
EIRKTIQHDVETNAAFQNPLFNDELKYWLDSKRYLMQPLQEMSPKMVSQLESSLLNCP
DSLDADSPCLYTKPLSLPHPTSIFFPNEPIRFVYPYDVPLNLTNNENDTDNKFGKDSK
AKSKKDDDIYSRTSLARIFMKFDLDTLFFIFYHYQGSYEQFLAARELFKNRNWLFNKV
DRCWYYKEIEKLPPGMGKSEEESWRYFDYKKSWLARRCGNDFVYNEEDFEKL"
gene complement(<691933..>692418)
/gene="LTP1"
/locus_tag="YPR073C"
/db_xref="GeneID:856187"
mRNA complement(<691933..>692418)
/gene="LTP1"
/locus_tag="YPR073C"
/product="tyrosine protein phosphatase LTP1"
/transcript_id="NM_001184170.1"
/db_xref="GeneID:856187"
CDS complement(691933..692418)
/gene="LTP1"
/locus_tag="YPR073C"
/EC_number="3.1.3.2"
/EC_number="3.1.3.48"
/experiment="EXISTENCE:direct assay:GO:0004725 protein
tyrosine phosphatase activity
[PMID:15358193|PMID:7629177]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006470 protein
dephosphorylation [PMID:7629177]"
/experiment="EXISTENCE:mutant phenotype:GO:0004725 protein
tyrosine phosphatase activity [PMID:7629177]"
/note="Protein phosphotyrosine phosphatase of unknown
cellular role; activated by adenine"
/codon_start=1
/product="tyrosine protein phosphatase LTP1"
/protein_id="NP_015398.1"
/db_xref="GeneID:856187"
/db_xref="SGD:S000006277"
/translation="MTIEKPKISVAFICLGNFCRSPMAEAIFKHEVEKANLENRFNKI
DSFGTSNYHVGESPDHRTVSICKQHGVKINHKGKQIKTKHFDEYDYIIGMDESNINNL
KKIQPEGSKAKVCLFGDWNTNDGTVQTIIEDPWYGDIQDFEYNFKQITYFSKQFLKKE
L"
gene complement(<692796..>694838)
/gene="TKL1"
/locus_tag="YPR074C"
/db_xref="GeneID:856188"
mRNA complement(<692796..>694838)
/gene="TKL1"
/locus_tag="YPR074C"
/product="transketolase TKL1"
/transcript_id="NM_001184171.1"
/db_xref="GeneID:856188"
CDS complement(692796..694838)
/gene="TKL1"
/locus_tag="YPR074C"
/EC_number="2.2.1.1"
/experiment="EXISTENCE:curator inference:GO:0005737
cytoplasm [PMID:8299150]"
/experiment="EXISTENCE:mutant phenotype:GO:0004802
transketolase activity [PMID:8226984]"
/experiment="EXISTENCE:mutant phenotype:GO:0006098
pentose-phosphate shunt [PMID:8299150]"
/note="Transketolase; catalyzes conversion of
xylulose-5-phosphate and ribose-5-phosphate to
sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate
in the pentose phosphate pathway; needed for synthesis of
aromatic amino acids; TKL1 has a paralog, TKL2, that arose
from the whole genome duplication"
/codon_start=1
/product="transketolase TKL1"
/protein_id="NP_015399.1"
/db_xref="GeneID:856188"
/db_xref="SGD:S000006278"
/translation="MTQFTDIDKLAVSTIRILAVDTVSKANSGHPGAPLGMAPAAHVL
WSQMRMNPTNPDWINRDRFVLSNGHAVALLYSMLHLTGYDLSIEDLKQFRQLGSRTPG
HPEFELPGVEVTTGPLGQGISNAVGMAMAQANLAATYNKPGFTLSDNYTYVFLGDGCL
QEGISSEASSLAGHLKLGNLIAIYDDNKITIDGATSISFDEDVAKRYEAYGWEVLYVE
NGNEDLAGIAKAIAQAKLSKDKPTLIKMTTTIGYGSLHAGSHSVHGAPLKADDVKQLK
SKFGFNPDKSFVVPQEVYDHYQKTILKPGVEANNKWNKLFSEYQKKFPELGAELARRL
SGQLPANWESKLPTYTAKDSAVATRKLSETVLEDVYNQLPELIGGSADLTPSNLTRWK
EALDFQPPSSGSGNYSGRYIRYGIREHAMGAIMNGISAFGANYKPYGGTFLNFVSYAA
GAVRLSALSGHPVIWVATHDSIGVGEDGPTHQPIETLAHFRSLPNIQVWRPADGNEVS
AAYKNSLESKHTPSIIALSRQNLPQLEGSSIESASKGGYVLQDVANPDIILVATGSEV
SLSVEAAKTLAAKNIKARVVSLPDFFTFDKQPLEYRLSVLPDNVPIMSVEVLATTCWG
KYAHQSFGIDRFGASGKAPEVFKFFGFTPEGVAERAQKTIAFYKGDKLISPLKKAF"
rep_origin 695461..695574
/note="ARS1625; Autonomously Replicating Sequence"
/db_xref="SGD:S000118421"
gene complement(<695737..>696819)
/gene="OPY2"
/locus_tag="YPR075C"
/db_xref="GeneID:856191"
mRNA complement(<695737..>696819)
/gene="OPY2"
/locus_tag="YPR075C"
/product="Opy2p"
/transcript_id="NM_001184172.1"
/db_xref="GeneID:856191"
CDS complement(695737..696819)
/gene="OPY2"
/locus_tag="YPR075C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:22842922|PMID:16543225]"
/experiment="EXISTENCE:genetic interaction:GO:0007231
osmosensory signaling pathway [PMID:16543225]"
/experiment="EXISTENCE:genetic interaction:GO:0072659
protein localization to plasma membrane
[PMID:17627274|PMID:22904036]"
/experiment="EXISTENCE:mutant phenotype:GO:0000751 mitotic
cell cycle G1 arrest in response to pheromone
[PMID:9383053]"
/experiment="EXISTENCE:mutant phenotype:GO:1900436
positive regulation of filamentous growth of a population
of unicellular organisms in response to starvation
[PMID:22904036]"
/experiment="EXISTENCE:physical interaction:GO:0007231
osmosensory signaling pathway [PMID:16543225]"
/note="Integral membrane protein that acts as a membrane
anchor for Ste50p; involved in the signaling branch of the
high-osmolarity glycerol (HOG) pathway and as a regulator
of the filamentous growth pathway; overproduction blocks
cell cycle arrest in the presence of mating pheromone;
relocalizes from vacuole to plasma membrane upon DNA
replication stress"
/codon_start=1
/product="Opy2p"
/protein_id="NP_015400.1"
/db_xref="GeneID:856191"
/db_xref="SGD:S000006279"
/translation="MSSSSKASASSSLSSTATSSTSATRGSDGCVVCDSTASCPVCAS
GEYCVMTSLTCDKCPSTYCAKQSDSQLSSLSSSSSSSSSSNSNEKTSLIVGFTVGIVG
GAMLIALVALYFINKRYWKPKRQKNKALKLEEASQSYGNEEEYFDDEDDDDEDDEDDG
GMRKDESHTLFNTSLVPPTLNVPGNRSSASTTRTRASNILPIAYIPGVTSGLSTDKLQ
SKLRSSSKRQNAAGDIRSHITLGSSILDGLDDEDDEHNQVLNKDADDNLITAIRAKPK
LVQIAEEESDKEIQDLDVIEEQTEADDLSHMAKSEASHGNNDEDDDEEGSFILDLEIP
ESIRESTQGSRTESPFEDKFEIHDER"
gene complement(<697147..>698265)
/locus_tag="YPR078C"
/db_xref="GeneID:856193"
mRNA complement(<697147..>698265)
/locus_tag="YPR078C"
/product="uncharacterized protein"
/transcript_id="NM_001184175.1"
/db_xref="GeneID:856193"
CDS complement(697147..698265)
/locus_tag="YPR078C"
/note="hypothetical protein; possible role in DNA
metabolism and/or in genome stability; expression is
heat-inducible"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015403.1"
/db_xref="GeneID:856193"
/db_xref="SGD:S000006282"
/translation="MQTISGVLPTVLSPSELRSDDERTFQFDEEAEITTHLTESEDLR
RLINETAQLGVRVDHIHDKTDQEIARLEKVIKEVTESDTFFRSCSGWFKTNKNFSDSE
SSSNTQLKSLSQLHGRYDRDWRQRLNKWFRKNKSKLALPSDNNLEEVNDDKVYGYGED
LMERGKTPYFSDIDDFMNGLNIISPLTPDDFENDDTLVKIDETCQIHSASEPEKTSIS
PTFGKNIKKELVTDDTESIISGPPLQENKKTLLKYRYVRTSLDMLGSEKSSSKNNSGG
MFRIFHKSANFGDKNQENVPRVWDTLRNNLGREIYLLQGRFKKWTTKHQNLKKGQPCK
DEDAVTVPLPSSDPGKETQLETKLCFVPEPGDQPLVQA"
gene <698869..>700014
/gene="MRL1"
/locus_tag="YPR079W"
/db_xref="GeneID:856194"
mRNA <698869..>700014
/gene="MRL1"
/locus_tag="YPR079W"
/product="Mrl1p"
/transcript_id="NM_001184176.1"
/db_xref="GeneID:856194"
CDS 698869..700014
/gene="MRL1"
/locus_tag="YPR079W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005770 late
endosome [PMID:11470415]"
/experiment="EXISTENCE:genetic interaction:GO:0005048
signal sequence binding [PMID:11470415]"
/experiment="EXISTENCE:mutant phenotype:GO:0007034
vacuolar transport [PMID:11470415]"
/note="Membrane protein; has similarity to mammalian
mannose-6-phosphate receptors; possibly functions as a
sorting receptor in the delivery of vacuolar hydrolases;
protein abundance increases in response to DNA replication
stress"
/codon_start=1
/product="Mrl1p"
/protein_id="NP_015404.1"
/db_xref="GeneID:856194"
/db_xref="SGD:S000006283"
/translation="MLKRSSLIYLSCVLIITIPILLHVYNGPGLSHEANEHRASHKQK
RTLANPDKPKSENDEDLFCAVTNPVTGSYIDLSQLSSTPNKLREGQKQISGNNKHESS
KTKWSVRGWGYDTNFTLGICSSPVGEAESQQLSNLTGAFYVDQLNENNLVSIGDFSTR
PALVGGSTAKKLTLKYENGSMCPNGKDKKATLLNFVCDKEIQSKAQISYIGNLHNCSY
FFEVRSIHACPTSNKKNEVNVLGIFIGIFAIFFLVEFAGRRWIYAKLNRHLKNDDELH
DISPSLNEQPHWDLIEDGSRWSKFFNGIIKTTRRFTKSLMRSLVRGRNSRQGGIRLRS
SPSASSSSLANREFFRDMEAQNEIIDSLDINSHTTESDHPTLADNSV"
gene <700594..>701970
/gene="TEF1"
/locus_tag="YPR080W"
/db_xref="GeneID:856195"
mRNA <700594..>701970
/gene="TEF1"
/locus_tag="YPR080W"
/product="translation elongation factor EF-1 alpha"
/transcript_id="NM_001184177.1"
/db_xref="GeneID:856195"
CDS 700594..701970
/gene="TEF1"
/locus_tag="YPR080W"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:25561732]"
/experiment="EXISTENCE:direct assay:GO:0005525 GTP binding
[PMID:17925388]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:20032305]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0019003 GDP binding
[PMID:17925388]"
/experiment="EXISTENCE:direct assay:GO:0051015 actin
filament binding [PMID:25561732]"
/experiment="EXISTENCE:direct assay:GO:0051017 actin
filament bundle assembly
[PMID:19095653|PMID:16116436|PMID:25561732]"
/experiment="EXISTENCE:direct assay:GO:1904408 melatonin
binding [PMID:31708896]"
/experiment="EXISTENCE:genetic interaction:GO:0006409 tRNA
export from nucleus [PMID:10766739|PMID:17203074]"
/experiment="EXISTENCE:mutant phenotype:GO:0003746
translation elongation factor activity [PMID:10514524]"
/experiment="EXISTENCE:mutant phenotype:GO:0006409 tRNA
export from nucleus [PMID:17203074|PMID:10766739]"
/experiment="EXISTENCE:mutant phenotype:GO:0006414
translational elongation [PMID:10514524]"
/experiment="EXISTENCE:mutant phenotype:GO:0051017 actin
filament bundle assembly [PMID:16116436]"
/experiment="EXISTENCE:physical interaction:GO:0005739
mitochondrion [PMID:16962558]"
/note="Translational elongation factor EF-1 alpha;
GTP-bound active form, binds to and delivers aminoacylated
tRNA to the A-site of ribosomes for elongation of nascent
polypeptides; moonlighting function as an actin binding
and bundling protein; association with GTPase Rho1p on the
vacuolar membrane may facilitate F-actin remodeling;
involved in tRNA re-export from the nucleus"
/codon_start=1
/product="translation elongation factor EF-1 alpha"
/protein_id="NP_015405.1"
/db_xref="GeneID:856195"
/db_xref="SGD:S000006284"
/translation="MGKEKSHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEK
EAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYQVTVIDAPGHRDFIKNM
ITGTSQADCAILIIAGGVGEFEAGISKDGQTREHALLAFTLGVRQLIVAVNKMDSVKW
DESRFQEIVKETSNFIKKVGYNPKTVPFVPISGWNGDNMIEATTNAPWYKGWEKETKA
GVVKGKTLLEAIDAIEQPSRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMVV
TFAPAGVTTEVKSVEMHHEQLEQGVPGDNVGFNVKNVSVKEIRRGNVCGDAKNDPPKG
CASFNATVIVLNHPGQISAGYSPVLDCHTAHIACRFDELLEKNDRRSGKKLEDHPKFL
KSGDAALVKFVPSKPMCVEAFSEYPPLGRFAVRDMRQTVAVGVIKSVDKTEKAAKVTK
AAQKAAKK"
gene complement(<702114..>703970)
/gene="GRS2"
/locus_tag="YPR081C"
/db_xref="GeneID:856196"
mRNA complement(<702114..>703970)
/gene="GRS2"
/locus_tag="YPR081C"
/product="putative glycine--tRNA ligase"
/transcript_id="NM_001184178.1"
/db_xref="GeneID:856196"
CDS complement(702114..703970)
/gene="GRS2"
/locus_tag="YPR081C"
/EC_number="6.1.1.14"
/experiment="EXISTENCE:direct assay:GO:0004820
glycine-tRNA ligase activity
[PMID:22438917|PMID:21877692]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:21877692]"
/experiment="EXISTENCE:genetic interaction:GO:0004820
glycine-tRNA ligase activity [PMID:22438917]"
/experiment="EXISTENCE:genetic interaction:GO:0006426
glycyl-tRNA aminoacylation [PMID:22438917]"
/note="Glycine-tRNA synthetase, not expressed under normal
growth conditions; expression is induced under heat,
oxidative, pH, or ethanol stress conditions; more stable
than the major glycine-tRNA synthetase Grs1p at 37 deg C;
GRS2 has a paralog, GRS1, that arose from the whole genome
duplication"
/codon_start=1
/product="putative glycine--tRNA ligase"
/protein_id="NP_015406.1"
/db_xref="GeneID:856196"
/db_xref="SGD:S000006285"
/translation="MPLMSNSERDKLESTLRRRFFYTPSFEIYGGVSGLFDLGPPGCQ
LQNNLIRLWREHFIMEENMLQVDGPMLTPYDVLKTSGHVDKFTDWMCRNPKTGEYYRA
DHLIEQTLKKRLLDKDVNPQDMKNMEKILTTIDGFSGPELNLVMQEYNINDPVTNDVL
DALTSFNLMFETKIGASGQLKAFLRPETAQGQFLNFNKLLEINQGKIPFASASIGKSF
RNEISPRSGLLRVREFLMAEIEHFVDPLNKSHAKFNEVLNEEIPLLSRRLQESGEVQL
PVKMTIGEAVNSGMVENETLGYFMARVHQFLLNIGINKDKFRFRQHLKNEMAHYATDC
WDGEILTSYGWIECVGCADRAAFDLTVHSKKTGRSLTVKQKLDTPKERTEWVVEVNKK
FFGSKFKQKAKLIESVLSKFSQDELIRRHEELEKNGEFTCQVNGQIVKLDSSLVTIKM
KTTLQHIREYIPNVIEPSFGLGRIIYCIFDHCFQVRVDSESRGFFSFPLQIAPIKVFV
TTISNNDGFPAILKRISQALRKREIYFKIDDSNTSIGKKYARNDELGTPFGITIDFET
IKDQTVTLRERNSMRQVRGTITDVISTIDKMLHNPDESDWDKSTFGLSPVKI"
gene complement(<704225..>704656)
/gene="DIB1"
/locus_tag="YPR082C"
/gene_synonym="SNU16"
/db_xref="GeneID:856197"
mRNA complement(<704225..>704656)
/gene="DIB1"
/locus_tag="YPR082C"
/gene_synonym="SNU16"
/product="U4/U6-U5 snRNP complex subunit DIB1"
/transcript_id="NM_001184179.1"
/db_xref="GeneID:856197"
CDS complement(704225..704656)
/gene="DIB1"
/locus_tag="YPR082C"
/gene_synonym="SNU16"
/experiment="EXISTENCE:direct assay:GO:0005682 U5 snRNP
[PMID:11720284]"
/experiment="EXISTENCE:direct assay:GO:0046540 U4/U6 x U5
tri-snRNP complex [PMID:10449419|PMID:10377396]"
/experiment="EXISTENCE:mutant phenotype:GO:0000398 mRNA
splicing, via spliceosome [PMID:10610776|PMID:11720284]"
/note="17-kDa component of the U4/U6aU5 tri-snRNP; plays
an essential role in pre-mRNA splicing; human ortholog
TXNL4A (the human U5-specific 15-kDa protein) complements
yeast dib1 null mutant"
/codon_start=1
/product="U4/U6-U5 snRNP complex subunit DIB1"
/protein_id="NP_015407.1"
/db_xref="GeneID:856197"
/db_xref="SGD:S000006286"
/translation="MASVLLPQLRTGWHVDQAIVTETKRLVVIRFGRKNDRQCMIMDE
LLSSIAERVRNFAVIYLCDIDEVSDFDEMYELTDPMTVMFFYHNKHMMCDFGTGNNNK
LNFIVDDKQEMIDILETIFRGARKNKGLVVSPYDYNHKRVS"
gene <704854..>706593
/gene="MDM36"
/locus_tag="YPR083W"
/db_xref="GeneID:856198"
mRNA <704854..>706593
/gene="MDM36"
/locus_tag="YPR083W"
/product="Mdm36p"
/transcript_id="NM_001184180.1"
/db_xref="GeneID:856198"
CDS 704854..706593
/gene="MDM36"
/locus_tag="YPR083W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:20505073]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:20505073]"
/experiment="EXISTENCE:mutant phenotype:GO:0000001
mitochondrion inheritance [PMID:23641071]"
/experiment="EXISTENCE:mutant phenotype:GO:0000266
mitochondrial fission [PMID:20505073]"
/experiment="EXISTENCE:mutant phenotype:GO:0007005
mitochondrion organization [PMID:11907266]"
/experiment="EXISTENCE:mutant phenotype:GO:0048312
intracellular distribution of mitochondria
[PMID:20505073]"
/note="Mitochondrial protein; required for normal
mitochondrial morphology and inheritance; component of the
mitochondria-ER-cortex-anchor (MECA); interacts with Num1p
to link the ER and mitochondria at the cell cortex;
proposed involvement in the formation of Dnm1p and
Num1p-containing cortical anchor complexes that promote
mitochondrial fission"
/codon_start=1
/product="Mdm36p"
/protein_id="NP_015408.1"
/db_xref="GeneID:856198"
/db_xref="SGD:S000006287"
/translation="MDENGTVKPGYELKGLNSGNSRSNMDKDPIVSKFHRAGLNDSAD
EEDTDINGNRNTSWITSMISEEKRKVEGKSMLNDEEDLHLSKATLNKCDALVKILADI
IKLEFVIHQSWYIRSLHKSVLIQFEVETSGGNKNSAGDSGDDDDDNHNGNLDDSFYKD
LSLKCIKKCEKSSLALESLSRDIDQIRDFIMSNTIEDNRVDRLLQNSMTLLLECWIYS
MKRLRRLRMKIAGIFVRSKLLLIDHELVTIWHFLQEQNEHETVNNENELKLAETIKSY
RAFIKIFIQQLEDSESGSPSSSLFEECLHVFLDIESMYNSLNLNWLLNENKALQERLL
SPSSTSENDHTNNLPVIDETKEIEDISSFVNSIVDASMLTHDLTPINSSDSDNLSNGE
IDRLDGRRLSSSTSDMSLMMQRTSLQKQLPSLLTAFNNARRLEQELQNACKVNDNKHS
TKDTDSNIRRNEHAMSSSVSSIISQNSTLASPSPPMSSSFISTAPSQSSSRMSTLPLS
PSSSLLESQSQTLKNNMSQWLNQSRSGLNGTKLIPTNHIGFHSNVLNTLYGIGGGPVS
KSYKSNQPSSQNT"
gene <706972..>708342
/locus_tag="YPR084W"
/db_xref="GeneID:856199"
mRNA <706972..>708342
/locus_tag="YPR084W"
/product="uncharacterized protein"
/transcript_id="NM_001184181.1"
/db_xref="GeneID:856199"
CDS 706972..708342
/locus_tag="YPR084W"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015409.1"
/db_xref="GeneID:856199"
/db_xref="SGD:S000006288"
/translation="MSEKASEERPIRLAVLGGTSTGKTSLVSRLTVNIVHEVHYPTRN
QTNWLFGFVPSSILARAILDEQAHERLCLRSPSSQTLEPIFPSPQVSKNVLLSPLVFQ
ASTDNFQSVRLHNKSHSRRSLSLDKSDSPLYQTFSNDINSQSVPKIKADQLNVIEHFK
LPLNYIPPTYAPIQIDIIDTPGFSPDNVVPFLEVSLFRNLGKSILHGLADEPRRPVST
TSLLVASGASELNGKVDGYILVYSAVPELNHIGGPPEYGDDVMNTDTENVSDGGFELL
KVIRNCILDAWTEFRNYEKRWEEGKEDDIYSLVYSLRHLWSKNSKEKSAKIEQLRSYN
TKLKSIELDPSSPDSPPPCIIVCSHVNHELASPMLIEMGRQLATKWKYGFVGIDSMDD
LNVDVAVSLLIKEISEKMKLLVSNSNGSSSSGNSSSIYNSHLMNDKKKNNNAGLNKNM
LKKIIK"
gene complement(<708497..>709828)
/gene="ASA1"
/locus_tag="YPR085C"
/db_xref="GeneID:856200"
mRNA complement(<708497..>709828)
/gene="ASA1"
/locus_tag="YPR085C"
/product="Asa1p"
/transcript_id="NM_001184182.1"
/db_xref="GeneID:856200"
CDS complement(708497..709828)
/gene="ASA1"
/locus_tag="YPR085C"
/experiment="EXISTENCE:direct assay:GO:0110078 TTT Hsp90
cochaperone complex [PMID:19040720]"
/note="Subunit of the TTT complex and regulator of
telomere length; subunit of the TTT Hsp90 cochaperone
complex that is involved in chromatin remodeling and
telomeric chromatin regulation; telomere length regulator
involved in the stability or biogenesis of PIKKs such as
TORC1"
/codon_start=1
/product="Asa1p"
/protein_id="NP_015410.2"
/db_xref="GeneID:856200"
/db_xref="SGD:S000006289"
/translation="MRGFSNEIILKRTLTLSDFTLRYHKRGITALQVIKAPSVSNVPV
LLSGDNYGYFVMWDLVTKRPITHIEIEGNSHIIAFWWVETTNVLYILSKDSMLRIFEL
DSSTQLSIDLVRKLSQANKTDHLQWTKIYEMPINTLNFANFIIEAEVKPTKDNKSYRL
VCCHTDDSETIDIYQIIEDSTFKLKRPFNNINFPRFLKQQNFLGISKDSKFGIIMRFA
KLNDVIFLGYENGFVVGFKITFDEGLQRDIAELVHVSNDHYPNPILDMCVSGDELYSC
STDDFITKYKIPVNLQLETKYLRDDALLIKCPSSLRVSEPSKVHLPLKNIGHIDKVKD
DYLVVSSWSGMTIVYNMRTSEVEQTFVKSKNNLVVSDSSMGDLTNGSGSNTESSSKSH
NYKVGAMTCLESFDVQSDGLRLGQLRRIKALAKCNWCLIGYEDGTIKLNKI"
gene <710101..>711138
/gene="SUA7"
/locus_tag="YPR086W"
/gene_synonym="SOH4"
/db_xref="GeneID:856201"
mRNA <710101..>711138
/gene="SUA7"
/locus_tag="YPR086W"
/gene_synonym="SOH4"
/product="transcription factor SUA7"
/transcript_id="NM_001184183.1"
/db_xref="GeneID:856201"
CDS 710101..711138
/gene="SUA7"
/locus_tag="YPR086W"
/gene_synonym="SOH4"
/experiment="EXISTENCE:direct assay:GO:0000993 RNA
polymerase II complex binding [PMID:7935466]"
/experiment="EXISTENCE:direct assay:GO:0001139 RNA
polymerase II complex recruiting activity [PMID:10464320]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:15888545]"
/experiment="EXISTENCE:direct assay:GO:0017025 TBP-class
protein binding [PMID:10464320|PMID:15888545]"
/experiment="EXISTENCE:direct assay:GO:0051123 RNA
polymerase II preinitiation complex assembly
[PMID:10464320]"
/experiment="EXISTENCE:direct assay:GO:0097550
transcription preinitiation complex [PMID:22751016]"
/experiment="EXISTENCE:direct assay:GO:2000679 positive
regulation of transcription regulatory region DNA binding
[PMID:8132551]"
/experiment="EXISTENCE:mutant phenotype:GO:0001113
transcription open complex formation at RNA polymerase II
promoter [PMID:19820686]"
/experiment="EXISTENCE:mutant phenotype:GO:0001139 RNA
polymerase II complex recruiting activity [PMID:10464320]"
/experiment="EXISTENCE:mutant phenotype:GO:0001174
transcriptional start site selection at RNA polymerase II
promoter [PMID:19820686]"
/experiment="EXISTENCE:mutant phenotype:GO:0017025
TBP-class protein binding [PMID:10464320]"
/experiment="EXISTENCE:mutant phenotype:GO:0051123 RNA
polymerase II preinitiation complex assembly
[PMID:10464320|PMID:9887099]"
/experiment="EXISTENCE:physical interaction:GO:0000993 RNA
polymerase II complex binding [PMID:7935466]"
/experiment="EXISTENCE:physical interaction:GO:0017025
TBP-class protein binding [PMID:15888545]"
/note="Transcription factor TFIIB; a general transcription
factor required for transcription initiation and start
site selection by RNA polymerase II"
/codon_start=1
/product="transcription factor SUA7"
/protein_id="NP_015411.1"
/db_xref="GeneID:856201"
/db_xref="SGD:S000006290"
/translation="MMTRESIDKRAGRRGPNLNIVLTCPECKVYPPKIVERFSEGDVV
CALCGLVLSDKLVDTRSEWRTFSNDDHNGDDPSRVGEASNPLLDGNNLSTRIGKGETT
DMRFTKELNKAQGKNVMDKKDNEVQAAFAKITMLCDAAELPKIVKDCAKEAYKLCHDE
KTLKGKSMESIMAASILIGCRRAEVARTFKEIQSLIHVKTKEFGKTLNIMKNILRGKS
EDGFLKIDTDNMSGAQNLTYIPRFCSHLGLPMQVTTSAEYTAKKCKEIKEIAGKSPIT
IAVVSIYLNILLFQIPITAAKVGQTLQVTEGTIKSGYKILYEHRDKLVDPQLIANGVV
SLDNLPGVEKK"
gene complement(<711403..>713028)
/gene="SRP54"
/locus_tag="YPR088C"
/gene_synonym="SRH1"
/db_xref="GeneID:856203"
mRNA complement(<711403..>713028)
/gene="SRP54"
/locus_tag="YPR088C"
/gene_synonym="SRH1"
/product="RNA-binding signal recognition particle subunit
SRP54"
/transcript_id="NM_001184185.1"
/db_xref="GeneID:856203"
CDS complement(711403..713028)
/gene="SRP54"
/locus_tag="YPR088C"
/gene_synonym="SRH1"
/EC_number="3.6.5.4"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005786 signal
recognition particle, endoplasmic reticulum targeting
[PMID:7925282]"
/experiment="EXISTENCE:direct assay:GO:0006614
SRP-dependent cotranslational protein targeting to
membrane [PMID:10921896]"
/experiment="EXISTENCE:direct assay:GO:0008312 7S RNA
binding [PMID:14681587]"
/experiment="EXISTENCE:mutant phenotype:GO:0006614
SRP-dependent cotranslational protein targeting to
membrane [PMID:1655273]"
/note="Signal recognition particle (SRP) subunit (homolog
of mammalian SRP54); contains the signal sequence-binding
activity of SRP, interacts with the SRP RNA, and mediates
binding of SRP to signal receptor; contains GTPase domain"
/codon_start=1
/product="RNA-binding signal recognition particle subunit
SRP54"
/protein_id="NP_015413.1"
/db_xref="GeneID:856203"
/db_xref="SGD:S000006292"
/translation="MVLADLGKRINSAVNNAISNTQDDFTTSVDVMLKGIVTALLESD
VNIALVSKLRNNIRSQLLSENRSEKSTTNAQTKKLIQKTVFDELCKLVTCEGSEEKAF
VPKKRKTNIIMFVGLQGSGKTTSCTKLAVYYSKRGFKVGLVCADTFRAGAFDQLKQNA
IRARIPFYGSYTETDPAKVAEEGINKFKKEKFDIIIVDTSGRHHQEEELFQEMIEISN
VIKPNQTIMVLDASIGQAAEQQSKAFKESSDFGAIILTKMDGHARGGGAISAVAATNT
PIIFIGTGEHIHDLEKFSPKSFISKLLGIGDIESLFEQLQTVSNKEDAKATMENIQKG
KFTLLDFKKQMQTIMKMGPLSNIAQMIPGMSNMMNQVGEEETSQKMKKMVYVLDSMTK
EELESDGRMFIEEPTRMVRVAKGSGTSVFEVEMILMQQQMMARMAQTATQQQPGAPGA
NARMPGMPNMPGMPNMPGMPNMPGMPKVTPQMMQQAQQKLKQNPGLMQNMMNMFGGGM
GGGMGGGMPDMNEMMKMMQDPQMQQMAKQFGMG"
gene <713275..>715941
/locus_tag="YPR089W"
/gene_synonym="YPR090W"
/db_xref="GeneID:856204"
mRNA <713275..>715941
/locus_tag="YPR089W"
/gene_synonym="YPR090W"
/product="uncharacterized protein"
/transcript_id="NM_001184186.1"
/db_xref="GeneID:856204"
CDS 713275..715941
/locus_tag="YPR089W"
/gene_synonym="YPR090W"
/note="hypothetical protein; exhibits genetic interaction
with ERG11 and protein-protein interaction with Hsp82p"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015414.2"
/db_xref="GeneID:856204"
/db_xref="SGD:S000006293"
/translation="MNSVWDDARIEDRTVDKPVGSSHAQEKLALVKSTLFKLDQEDRP
ECDSWVQLVKLICDEDREEEFTTFKELLREVKNVNDKSVTGVALIHYIIVFDRADYIE
LLHDNPSGAKLDLNLVDDIVGYTPLMWSFSLQRRNCCLELFNAFDEINFNMTNKAGLT
AWDMVPPYSPLSEFLEQNNMFRYRTEVKHEIPQISQPKDTSLLMSNEDSTTKETFDNI
DLQVAGLTLSPGANDNMFLDSDEKNMNHSQGAATLIDPTYTEDYHGTFDYDKLSPDQY
LEFSDFDIPQILNLLISLPQKEPHMTTYPAGLIYQCIRYADHKIKSKPLVESLINLSL
TKILTSVSSNGAAGLVSTEASLQAGDIVLQSYWLSCLSFLYYYLCRDDSFFKRHPSVL
QELINTIHSIIIELTSSIHCRLISLIDSTLLAYTTIQDVKQTLYKRDWNFFKKRKQAK
LLLKEKNRKQLKEQQKKELHRKSQGQENHEEEEGQQDGNDSDDRASTNDDNNSSVSLF
YDKEILRHLYPPSFEEQMKPSPLKIVQIFGALSYVLNLHQTHPIFQQQCLSISVNWFA
TTLFNKILKDKKKRSLSRAHAIQIRLNLSTLESWIQNNDFCVPKPMLIDDFMWQRFPM
TLIRDVGEIDLSDPILRNVATYKPIDENNKDLIYDTSNSLFYYQPFHKIAQIHLEPVF
QLLQWLQVATTLDSEESLISTMNLLPRITPVQLLKSMEKYNYELNENKFNSKLKKFLN
NKIKDSKMSKADAYLQEHEIPYLVLPTIPEMTDLYSKGPDSHSFQPFLPGSIQDDVYE
IHDVNFKQRQNEPQISRTNSGTSDFTGDEDKAQYETEGVGESIDINETVEPESNAFNV
GNDDYFKELNIPSSTAQRPAWSNNDDMEQNPW"
gene complement(<716156..>718468)
/gene="NVJ2"
/locus_tag="YPR091C"
/db_xref="GeneID:856207"
mRNA complement(<716156..>718468)
/gene="NVJ2"
/locus_tag="YPR091C"
/product="Nvj2p"
/transcript_id="NM_001184188.1"
/db_xref="GeneID:856207"
CDS complement(716156..718468)
/gene="NVJ2"
/locus_tag="YPR091C"
/experiment="EXISTENCE:direct assay:GO:0005319 lipid
transporter activity [PMID:28011845]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0008289 lipid
binding [PMID:22250200]"
/experiment="EXISTENCE:direct assay:GO:0033116 endoplasmic
reticulum-Golgi intermediate compartment membrane
[PMID:28011845]"
/experiment="EXISTENCE:direct assay:GO:0071561
nucleus-vacuole junction [PMID:22250200]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0035621 ER to
Golgi ceramide transport [PMID:28011845]"
/note="Lipid-binding ER protein, enriched at
nucleus-vacuolar junctions (NVJ); involved in nonvesicular
transfer of ceramides from ER to Golgi during ER stress;
may be involved in sterol metabolism or signaling at the
NVJ; contains a synaptotagmin-like-mitochondrial-lipid
binding protein (SMP) domain; binds phosphatidylinositols
and other lipids in a large-scale study; may interact with
ribosomes, based on co-purification experiments"
/codon_start=1
/product="Nvj2p"
/protein_id="NP_015416.1"
/db_xref="GeneID:856207"
/db_xref="SGD:S000006295"
/translation="MASLKVFLAVYLLGGITFLPLVLFTLYKIHLLYSNLKSASKKEL
DHDTADEIDEKTRLLARDIDPEFKARKLEEQLGVKVFNKGWITVTKQYYYHSSEVAVI
LKNSNNNKDSDTALQEQILQRTDLKKKQRFFAVLRHGNLFLYKDDSQNANLVHAISLQ
NRFITIWPRFDELGKEELPDASLFTKRTCIAIFKNDLVSIDSKNHNVILPHFDPLTSA
ESNNGDISTNDTTHEYQSQFHSSNQFFLYFDNNMDKEDWYYQLINASKNSNSLSTGLL
DPNVSANAAHLKTKDMLQLIQDINSTENQLTTKWLNALLGRLFLSLQQTDTLNKFIHE
KICKKLNKIKTPGFLDDLVVEKVDVGDSAPLFTSPELLELSPEGSTKIAIDVQYRGNL
TIIIATKASINLGSRFKQREVSLQLSIKIKEFSGPLLFLIKPPPSNRIWYAFRTEPIM
DFEIEPIVSSSKLSYNVVTNAIKSKFAEAVKESLVVPFMDDIVFYPTPNEVYRGGIWE
EQDPEAAARARTAAAASDMNNTSAKEHLEALQEGGMKTQSRIKKALRPERKKENLKDL
VDASGVATKTTTQTTVTTATNDDVSSSENSTKSRKYFKNSIKKIGRWYKDNVGNSSDT
EDMDEIDVQDKKNDDSADERESDNPILTSNPKMISNRRPVPRRPSQPLNTLSPKLEGR
KEKDTENFPVPPSASNMNASKMFANKENRKFSVSSNDSQNSLKNGDPHVKASKLESSQ
AFVKKTSQNRFNDGFFKQDLEFEEQREPKL"
gene complement(718700..718806)
/gene="SNR51"
/locus_tag="YNCP0013C"
/db_xref="GeneID:9164983"
ncRNA complement(718700..718806)
/ncRNA_class="snoRNA"
/gene="SNR51"
/locus_tag="YNCP0013C"
/product="SNR51"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:10024243]"
/experiment="EXISTENCE:curator inference:GO:0031428 box
C/D methylation guide snoRNP complex [PMID:10024243]"
/experiment="EXISTENCE:mutant phenotype:GO:0030562 rRNA
2'-O-ribose methylation guide activity [PMID:10024243]"
/experiment="EXISTENCE:mutant phenotype:GO:0031167 rRNA
methylation [PMID:10024243]"
/note="C/D box small nucleolar RNA (snoRNA); guides
2'-O-methylation of large subunit (LSU) rRNA at position
U2729 and small subunit (SSU) rRNA at position A100"
/transcript_id="NR_132268.1"
/db_xref="GeneID:9164983"
/db_xref="SGD:S000006442"
gene complement(718887..719050)
/gene="SNR70"
/locus_tag="YNCP0014C"
/db_xref="GeneID:9164984"
ncRNA complement(718887..719050)
/ncRNA_class="snoRNA"
/gene="SNR70"
/locus_tag="YNCP0014C"
/product="SNR70"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:10024243]"
/experiment="EXISTENCE:curator inference:GO:0031428 box
C/D methylation guide snoRNP complex [PMID:10024243]"
/note="C/D box small nucleolar RNA (snoRNA); predicted to
guide 2'-O-methylation of small subunit (SSU) rRNA at
position C1639"
/transcript_id="NR_132269.1"
/db_xref="GeneID:9164984"
/db_xref="SGD:S000006461"
gene complement(719148..719242)
/gene="SNR41"
/locus_tag="YNCP0015C"
/db_xref="GeneID:9164986"
ncRNA complement(719148..719242)
/ncRNA_class="snoRNA"
/gene="SNR41"
/locus_tag="YNCP0015C"
/product="SNR41"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:10024243|PMID:8674114]"
/experiment="EXISTENCE:curator inference:GO:0031428 box
C/D methylation guide snoRNP complex
[PMID:8674114|PMID:10024243]"
/experiment="EXISTENCE:mutant phenotype:GO:0030562 rRNA
2'-O-ribose methylation guide activity [PMID:10024243]"
/experiment="EXISTENCE:mutant phenotype:GO:0031167 rRNA
methylation [PMID:10024243]"
/note="C/D box small nucleolar RNA (snoRNA); guides
2'-O-methylation of small subunit (SSU) rRNA at positions
A541 and G1126"
/transcript_id="NR_132270.1"
/db_xref="GeneID:9164986"
/db_xref="SGD:S000007305"
gene complement(<719558..>720424)
/gene="ASR1"
/locus_tag="YPR093C"
/db_xref="GeneID:856208"
mRNA complement(<719558..>720424)
/gene="ASR1"
/locus_tag="YPR093C"
/product="ubiquitin-protein ligase ASR1"
/transcript_id="NM_001184190.1"
/db_xref="GeneID:856208"
CDS complement(719558..720424)
/gene="ASR1"
/locus_tag="YPR093C"
/experiment="EXISTENCE:direct assay:GO:0004842
ubiquitin-protein transferase activity [PMID:19064926]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:15117954]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:15117954]"
/experiment="EXISTENCE:direct assay:GO:0016567 protein
ubiquitination [PMID:19064926]"
/experiment="EXISTENCE:mutant phenotype:GO:0000921 septin
ring assembly [PMID:25646695]"
/experiment="EXISTENCE:mutant phenotype:GO:0016567 protein
ubiquitination [PMID:19064926]"
/experiment="EXISTENCE:mutant phenotype:GO:0045471
response to ethanol [PMID:15117954]"
/note="Ubiquitin ligase that modifies and regulates RNA
Pol II; involved in a putative alcohol-responsive
signaling pathway; accumulates in the nucleus under
alcohol stress; has a role in organization of septins and
the actin cytoskeleton; contains a Ring/PHD finger domain
similar to the mammalian rA9 protein"
/codon_start=1
/product="ubiquitin-protein ligase ASR1"
/protein_id="NP_015418.2"
/db_xref="GeneID:856208"
/db_xref="SGD:S000006297"
/translation="MEECPICLADDQEGEQFGCLNVCGHKFHLNCIREWHKYSINLKC
PICRVESTHLEVGEGQHALSINLKMGFMIKNAIDYVGAETTNERNEDDTGEQDQEIEF
LSERLRGTLVMDTIKIIQCSICGDTDVSRLSLYCQDCEAIYHETCLRGLACEVGDRNT
WQECTDCRSNALLELRMGAISSQLASYDSRNSMIFAGELRDKHSVKTQQMYEQIRNAK
HKIQMHVRRALDRYPLPLLRFKDAYKHVNKQVSRKLYRLSDNKYLPDQYDYDSLARTG
VHTELLIYCHDE"
gene <720637..>720960
/gene="RDS3"
/locus_tag="YPR094W"
/db_xref="GeneID:856209"
mRNA <720637..>720960
/gene="RDS3"
/locus_tag="YPR094W"
/product="U2 snRNP complex subunit RDS3"
/transcript_id="NM_001184191.1"
/db_xref="GeneID:856209"
CDS 720637..720960
/gene="RDS3"
/locus_tag="YPR094W"
/experiment="EXISTENCE:direct assay:GO:0000245
spliceosomal complex assembly [PMID:14517302]"
/experiment="EXISTENCE:direct assay:GO:0005686 U2 snRNP
[PMID:15565172|PMID:16314500]"
/experiment="EXISTENCE:genetic interaction:GO:0000398 mRNA
splicing, via spliceosome [PMID:12871902]"
/experiment="EXISTENCE:mutant phenotype:GO:0000245
spliceosomal complex assembly [PMID:14517302]"
/experiment="EXISTENCE:mutant phenotype:GO:0000398 mRNA
splicing, via spliceosome [PMID:12871902]"
/experiment="EXISTENCE:mutant phenotype:GO:0009410
response to xenobiotic stimulus [PMID:11943786]"
/experiment="EXISTENCE:physical interaction:GO:0000398
mRNA splicing, via spliceosome [PMID:16314500]"
/note="Component of the SF3b subcomplex of the U2 snRNP;
zinc cluster protein involved in pre-mRNA splicing and
cycloheximide resistance"
/codon_start=1
/product="U2 snRNP complex subunit RDS3"
/protein_id="NP_015419.1"
/db_xref="GeneID:856209"
/db_xref="SGD:S000006298"
/translation="MSRHQFDLIMCLKQPGVQTGLLCEKCDGKCPICDSYVRPKRKVR
VCENCSFGKQAKNCIICNLNVGVNDAFYCWECCRLGKDKDGCPRILNLGSNRLDRHFE
KKKKV"
gene complement(<721037..>724717)
/gene="SYT1"
/locus_tag="YPR095C"
/db_xref="GeneID:856210"
mRNA complement(<721037..>724717)
/gene="SYT1"
/locus_tag="YPR095C"
/product="Arf family guanine nucleotide exchange factor
SYT1"
/transcript_id="NM_001184192.1"
/db_xref="GeneID:856210"
CDS complement(721037..724717)
/gene="SYT1"
/locus_tag="YPR095C"
/experiment="EXISTENCE:direct assay:GO:0000139 Golgi
membrane [PMID:20841378]"
/experiment="EXISTENCE:direct assay:GO:0005085
guanyl-nucleotide exchange factor activity
[PMID:10430582]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0032014 positive
regulation of ARF protein signal transduction
[PMID:20841378]"
/experiment="EXISTENCE:genetic interaction:GO:0006887
exocytosis [PMID:10430582]"
/experiment="EXISTENCE:mutant phenotype:GO:0016192
vesicle-mediated transport [PMID:10430582]"
/experiment="EXISTENCE:mutant phenotype:GO:0032014
positive regulation of ARF protein signal transduction
[PMID:20841378]"
/experiment="EXISTENCE:mutant phenotype:GO:0034067 protein
localization to Golgi apparatus [PMID:26966233]"
/experiment="EXISTENCE:mutant phenotype:GO:0034976
response to endoplasmic reticulum stress [PMID:26966233]"
/note="Guanine nucleotide exchange factor (GEF) for Arf
proteins; promotes activation of Arl1p, which recruits
Imh1p to the Golgi; involved in vesicular transport;
member of the Sec7-domain family; contains a PH domain"
/codon_start=1
/product="Arf family guanine nucleotide exchange factor
SYT1"
/protein_id="NP_015420.1"
/db_xref="GeneID:856210"
/db_xref="SGD:S000006299"
/translation="MNQSISSLIKLKFLQSHSNDKNGNKKGGSNVSTGIDKLRESESY
RSPFLQLAEIQEHTNNDDDKLDVKECEPTKKHSKLSRIRRKMGRLDLNFRSANEKGSE
DDEILVAQHLRNGQDPEEMPFKSENNIDSIEKVPKPDGERVTLTSSGSDNVKRNSKHA
PFIPVKPALEKFPSSNRLSRDYRKSQEPTLFNGDRLVPTLPTVSRISTSSSVGSSTAA
SRYFNPSKRAVVASSSSSSSSIKFNSLHAIPLDATPQIELAKQQDEISKRRFGRRRSR
TVDVFDYINKNNTAKNKPPLSPSSFIRTIDEKNTNSLMQDPMGSRGPLLPDDANIISN
DTDGAEASHPDHQVLSRSRSQSTSFVQGKGGKRKSIEDEGYHNKLGLPHGSGPTSVYN
NKSNANSTITGMSRRSSSIVNALSSFVNLRSSSLSSSRQQHLQQQQQLQQKLDVSLED
LPPVPAPEFSDSCKDFLIKLAPYGKFIGIILTEKDDEFKKNCLNYLLTNCFEFKNDPL
DIALRKLLMFLELPKETQQIDRLIMAFSFAYYKAQKSYSKKKGIECPWSNADQVYFIA
FSLLMLHTDYFNPNNKSKMTKHDFVDLVHNDKYSGGNEIPMAVLTYFYENVTAKESPK
FNYFLMSPMALDDSILDKDAFDTNFAITLSSNSMYSPIDMIKRGSIIPKEASLSPIFY
PLTNSISASGIAPSTAASCPPSTSGTINGANLGTANSNSNRPASNSISSYFSYNPSSS
SSGNATLVQDDINVYSHIINDTLNEVNLFPEVSKYWNKNALKANLLRNEEHKYEKYYS
IMNDTKGGYLRFHKSQLNKLNLPNFEILNDNSRSGCKNSDYKYCKILQMGAIMNLGMP
SRKFSIVNSAKIHWKKEFAILTSLGLLICDKMDWINPQMMKDPKSGTTNYIIDFKSGF
SFVPGSTIDVYNGLFADRERDSLGKSHFASLVLAYTEHHSTGSHTSNTTAASSSAKHN
EGVFEPSSDEEDSITNSTDGTSSVSNGESDNDSVSSSDNQLSSNDSNEDYHSIKDEYP
IFEDENADCLLYLHTCHRNFIWKCANKYERDNWIDSINLFSAYDGCYVEIGSIANTIC
NKRKLTILQRMERLRSIKSAKWEKLKKFESTLMLMGKCVPISTKTKTDMINRIRQLAV
RMDWLIYEIKRSELFVSIIKEVTRKQAEKNILEHGKGEEEGQGNNDDSDGIDDIEESF
LFNENSLQVCVSDSSYDEYSNE"
gene complement(<724842..>725144)
/locus_tag="YPR096C"
/db_xref="GeneID:856211"
mRNA complement(<724842..>725144)
/locus_tag="YPR096C"
/product="uncharacterized protein"
/transcript_id="NM_001184193.1"
/db_xref="GeneID:856211"
CDS complement(724842..725144)
/locus_tag="YPR096C"
/experiment="EXISTENCE:mutant phenotype:GO:0045727
positive regulation of translation [PMID:32639961]"
/note="hypothetical protein; may interact with ribosomes,
based on co-purification experiments"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015421.1"
/db_xref="GeneID:856211"
/db_xref="SGD:S000006300"
/translation="MRGETGVSIKNPRPSRPFSCFWRKGDVENIRKSDIGNEKKIDAK
FNRLQYNLYYKPLSHHKAGLLYKELFFRSCFSYTTCSLDFQGKRHQVERKAVDIVL"
gene <725394..>728615
/gene="LEC1"
/locus_tag="YPR097W"
/db_xref="GeneID:856212"
mRNA <725394..>728615
/gene="LEC1"
/locus_tag="YPR097W"
/product="Lec1p"
/transcript_id="NM_001184194.2"
/db_xref="GeneID:856212"
CDS 725394..728615
/gene="LEC1"
/locus_tag="YPR097W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0035091
phosphatidylinositol binding [PMID:11557775]"
/note="Phosphoinositide-binding regulator of ergosterol
distribution; contains PX (Phox homology) domain;
associated with ER-lipid droplet contact site; may
facilitate ergosterol transport between plasma membrane
and lipid droplets"
/codon_start=1
/product="Lec1p"
/protein_id="NP_015422.2"
/db_xref="GeneID:856212"
/db_xref="SGD:S000006301"
/translation="MITQDTPALNPTEEHYLKRELLRCQLDYEIGKLNDQFALRKFGY
PFSPNDPTAPQPISNNDSSPVLGGKGHFSVNYPMLSYVLQEFISTFPLLSTNLLVDEK
FWQSKVQVFFEHFMSLGFSESYDREEASKRKKVSKKLSKVILLLFNSGVGSFQEQAYY
NEDKFVLQSGQARKRSNIEKFAMPTRENLENLLTNESVFINGWDVNIISVFNKNSRKC
TESVDNDKSSKSTPTSSPKSHAIKSFASTSKWMKNAFNNTINSTINSMPESSASLFSK
LSLGVPSTKSKQSRKHHYFLIKIKKQDDDDQDNSNEENSNLDHHAGYFYVTRTYSDFK
KLSHDLKSEFPGKKCPRLPHRNKKVTSMITKTEVLHNGQTKSAAREKIVNTFDTDLQS
ASESDNSSFLQTTNELSATETVLTEKETETLRKNILNEIKEEDNIDEDEYEEEGEGEE
SDFDEYKDASDSKINTLVGEKMRTSLRQYLRTLCKDAEVSQSSSIRRFFLSGPNLDIK
DINPKIADDIRNRALIDVSNLENQIRFQQMALEKSLKLQDSMKDFKTSLLKDEKYLMS
LLVEIKDNTKVEDLSPLLQDFVEWCKIYISSMIYQMFLGNDNSYELYTQIRRLHKLMP
YTVMGQIMKFTNPIAIMRGMIELFMAQPFGGHSLLQTMFSTILTDDLKTQKVAIKELE
RKIAEMDPGASVVTKCLKDFVFNNDTKDEHDTKLFTMDAVNAESESMNMPVPLIVLMK
SAAANLIPDEVVAGLIESYSSWKLQKEDTDALNVTSEDQSGIYFTHVKDLWQLYIKEH
DKQLMRQLWQDPELTQMLKAIVTMIYEPMVKIFKVARMDVALKNFEKFMSDLIRLVDD
VINGQLGVSTQFDVVEEIHNLVTKHQDAFFEFIHDVYLNDSEGIFEGFITWITTIVKF
LQKSKFGGPSERIDFNKLICRDDIDIDVKLLKVQVNNVLNKKIGARKIYKKLLDLKVK
QGTKQNNKHAAGILQKNWSDINSLVMPSSSGSFGLGDGDLVDLDLDTGDYDFLHKENE
VELEKQYKDLLNLVVDESEIDKLRSQVFAQELKNYLEAQIAKK"
gene complement(<728947..>729528)
/gene="TMH18"
/locus_tag="YPR098C"
/db_xref="GeneID:856213"
mRNA complement(join(<728947..729385,729482..>729528))
/gene="TMH18"
/locus_tag="YPR098C"
/product="Tmh18p"
/transcript_id="NM_001184195.1"
/db_xref="GeneID:856213"
CDS complement(join(728947..729385,729482..729528))
/gene="TMH18"
/locus_tag="YPR098C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:16407407]"
/note="Mitochondrial hypothetical protein; localized to
the mitochondrial outer membrane"
/codon_start=1
/product="Tmh18p"
/protein_id="NP_015423.2"
/db_xref="GeneID:856213"
/db_xref="SGD:S000006302"
/translation="MCLVKTTAHLLFYSFVFGGTTFYSYVASPIAFKVLEKDQFSALQ
NKIFPYFFQMQAASPVILALTAPIALTTGPLSSLVVASVSGLTNLFWLLPWTHKVKEQ
RKNIAKKYTGSELEAKDAILRKEFGKSHGLSLLFNLSNVCGMLAYGVCLSGGLLRKIP
K"
gene complement(<729757..>730113)
/locus_tag="YPR099C"
/db_xref="GeneID:856215"
mRNA complement(<729757..>730113)
/locus_tag="YPR099C"
/product="uncharacterized protein"
/transcript_id="NM_001387383.1"
/db_xref="GeneID:856215"
CDS complement(729757..730113)
/locus_tag="YPR099C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:31374566]"
/note="Mitochondrial hypothetical protein; null mutant has
a respiratory growth defect and displays reduced
peroxide-induced mitochondrial ROS; partially overlaps the
verified gene MRPL51/YPR100W"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001374312.1"
/db_xref="GeneID:856215"
/db_xref="SGD:S000006303"
/translation="MSPSESFNSFSLFSTLSMFKFLTQITFSRPFVYSALNKGCPDFL
ITSNCIHGNSWPHLSNLFEVKNFLMPSEDPPQLQNCKVIFLHGNTNAPTPLRPMEFRA
IAFTTISPYIRVCVST"
gene <729791..>730213
/gene="MRPL51"
/locus_tag="YPR100W"
/gene_synonym="mL43"
/db_xref="GeneID:856214"
mRNA <729791..>730213
/gene="MRPL51"
/locus_tag="YPR100W"
/gene_synonym="mL43"
/product="mitochondrial 54S ribosomal protein mL43 MRPL51"
/transcript_id="NM_001184197.1"
/db_xref="GeneID:856214"
CDS 729791..730213
/gene="MRPL51"
/locus_tag="YPR100W"
/gene_synonym="mL43"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:12392552]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:12392552]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:31374566|PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005762
mitochondrial large ribosomal subunit [PMID:12392552]"
/experiment="EXISTENCE:mutant phenotype:GO:0045454 cell
redox homeostasis [PMID:31374566]"
/note="Mitochondrial ribosomal protein of the large
subunit; required for mitochondrial genome integrity,
respiratory growth, and mitochondrial redox homeostasis,
partially overlaps verified gene YPR099C"
/codon_start=1
/product="mitochondrial 54S ribosomal protein mL43 MRPL51"
/protein_id="NP_015425.1"
/db_xref="GeneID:856214"
/db_xref="SGD:S000006304"
/translation="MVVKAIARNSIGRNGVGAFVFPCRKITLQFCNWGGSSEGMRKFL
TSKRLDKWGQEFPWIQFEVMRKSGHPLLRAEYTNGREKVICVRNLNIDNVENKLKLLK
DSDGDILRRRTKNDNVESLNSSVRGIWSPLHAAKRHRI"
gene <730492..>731019
/gene="SNT309"
/locus_tag="YPR101W"
/gene_synonym="NTC25"
/db_xref="GeneID:856216"
mRNA <730492..>731019
/gene="SNT309"
/locus_tag="YPR101W"
/gene_synonym="NTC25"
/product="Snt309p"
/transcript_id="NM_001184198.1"
/db_xref="GeneID:856216"
CDS 730492..731019
/gene="SNT309"
/locus_tag="YPR101W"
/gene_synonym="NTC25"
/experiment="EXISTENCE:curator inference:GO:0000384 first
spliceosomal transesterification activity [PMID:9528791]"
/experiment="EXISTENCE:direct assay:GO:0000974 Prp19
complex [PMID:9528791]"
/experiment="EXISTENCE:direct assay:GO:0071006 U2-type
catalytic step 1 spliceosome [PMID:9528791]"
/experiment="EXISTENCE:genetic interaction:GO:0000398 mRNA
splicing, via spliceosome [PMID:9528791]"
/experiment="EXISTENCE:mutant phenotype:GO:0000398 mRNA
splicing, via spliceosome [PMID:9528791]"
/note="Member of the NineTeen Complex (NTC); this complex
contains Prp19p and stabilizes U6 snRNA in catalytic forms
of the spliceosome containing U2, U5, and U6 snRNAs;
interacts physically and genetically with Prp19p"
/codon_start=1
/product="Snt309p"
/protein_id="NP_015426.1"
/db_xref="GeneID:856216"
/db_xref="SGD:S000006305"
/translation="MDGLSFVDKGKIPDGYKNEIDQLVKKEFANIKREPVHPEIRGIL
AKRKGADNSVSTLTNALYTEYLKQRNNKKRRTPDFNDDDDTLFLEEYRRKYPRIDTSR
YIPNESSEVSLLGIVDSYLKHQEIVLDTLLPQTVSNQWRINNDYIRQTCTIVEEMNIQ
QRKQINDLEIYRKRL"
gene complement(<731224..>731748)
/gene="RPL11A"
/locus_tag="YPR102C"
/db_xref="GeneID:856217"
mRNA complement(<731224..>731748)
/gene="RPL11A"
/locus_tag="YPR102C"
/product="60S ribosomal protein uL5 RPL11A"
/transcript_id="NM_001184199.1"
/db_xref="GeneID:856217"
CDS complement(731224..731748)
/gene="RPL11A"
/locus_tag="YPR102C"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:11983894]"
/experiment="EXISTENCE:curator inference:GO:0003735
structural constituent of ribosome [PMID:11983894]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:11983894]"
/experiment="EXISTENCE:mutant phenotype:GO:0000027
ribosomal large subunit assembly [PMID:2277060]"
/note="Ribosomal 60S subunit protein L11A; expressed at
twice the level of Rpl11Bp; involved in ribosomal
assembly; depletion causes degradation of 60S proteins and
RNA; homologous to mammalian ribosomal protein L11 and
bacterial L5; RPL11A has a paralog, RPL11B, that arose
from the whole genome duplication"
/codon_start=1
/product="60S ribosomal protein uL5 RPL11A"
/protein_id="NP_015427.1"
/db_xref="GeneID:856217"
/db_xref="SGD:S000006306"
/translation="MSAKAQNPMRDLKIEKLVLNISVGESGDRLTRASKVLEQLSGQT
PVQSKARYTVRTFGIRRNEKIAVHVTVRGPKAEEILERGLKVKEYQLRDRNFSATGNF
GFGIDEHIDLGIKYDPSIGIFGMDFYVVMNRPGARVTRRKRCKGTVGNSHKTTKEDTV
SWFKQKYDADVLDK"
gene <732349..>733212
/gene="PRE2"
/locus_tag="YPR103W"
/gene_synonym="DOA3; PRG1; SRR2"
/db_xref="GeneID:856218"
mRNA <732349..>733212
/gene="PRE2"
/locus_tag="YPR103W"
/gene_synonym="DOA3; PRG1; SRR2"
/product="proteasome core particle subunit beta 5"
/transcript_id="NM_001184200.1"
/db_xref="GeneID:856218"
CDS 732349..733212
/gene="PRE2"
/locus_tag="YPR103W"
/gene_synonym="DOA3; PRG1; SRR2"
/EC_number="3.4.25.1"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:36164978]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0010499 proteasomal
ubiquitin-independent protein catabolic process
[PMID:19162040]"
/experiment="EXISTENCE:direct assay:GO:0019774 proteasome
core complex, beta-subunit complex [PMID:9087403]"
/experiment="EXISTENCE:direct assay:GO:0043161
proteasome-mediated ubiquitin-dependent protein catabolic
process [PMID:19029916|PMID:11545745]"
/experiment="EXISTENCE:mutant phenotype:GO:0004175
endopeptidase activity [PMID:9312134|PMID:8808631]"
/experiment="EXISTENCE:mutant phenotype:GO:0080129
proteasome core complex assembly [PMID:8808631]"
/note="Beta 5 subunit of the 20S proteasome; responsible
for the chymotryptic activity of the proteasome; localizes
to peroxisomes in oleate-growing cells"
/codon_start=1
/product="proteasome core particle subunit beta 5"
/protein_id="NP_015428.1"
/db_xref="GeneID:856218"
/db_xref="SGD:S000006307"
/translation="MQAIADSFSVPNRLVKELQYDNEQNLESDFVTGASQFQRLAPSL
TVPPIASPQQFLRAHTDDSRNPDCKIKIAHGTTTLAFRFQGGIIVAVDSRATAGNWVA
SQTVKKVIEINPFLLGTMAGGAADCQFWETWLGSQCRLHELREKERISVAAASKILSN
LVYQYKGAGLSMGTMICGYTRKEGPTIYYVDSDGTRLKGDIFCVGSGQTFAYGVLDSN
YKWDLSVEDALYLGKRSILAAAHRDAYSGGSVNLYHVTEDGWIYHGNHDVGELFWKVK
EEEGSFNNVIG"
gene complement(<733623..>736433)
/gene="FHL1"
/locus_tag="YPR104C"
/gene_synonym="SPP42"
/db_xref="GeneID:856219"
mRNA complement(<733623..>736433)
/gene="FHL1"
/locus_tag="YPR104C"
/gene_synonym="SPP42"
/product="Fhl1p"
/transcript_id="NM_001184201.1"
/db_xref="GeneID:856219"
CDS complement(733623..736433)
/gene="FHL1"
/locus_tag="YPR104C"
/gene_synonym="SPP42"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:15692568|PMID:15620355]"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:27016735]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:15466158]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:15466158]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding
[PMID:19158363|PMID:19111667]"
/experiment="EXISTENCE:genetic interaction:GO:0010688
negative regulation of ribosomal protein gene
transcription by RNA polymerase II [PMID:7785326]"
/experiment="EXISTENCE:genetic interaction:GO:0060963
positive regulation of ribosomal protein gene
transcription by RNA polymerase II [PMID:15616569]"
/experiment="EXISTENCE:mutant phenotype:GO:0010688
negative regulation of ribosomal protein gene
transcription by RNA polymerase II [PMID:15620355]"
/experiment="EXISTENCE:mutant phenotype:GO:0060963
positive regulation of ribosomal protein gene
transcription by RNA polymerase II
[PMID:15620355|PMID:15616569]"
/experiment="EXISTENCE:physical interaction:GO:0001222
transcription corepressor binding [PMID:15620355]"
/experiment="EXISTENCE:physical interaction:GO:0001223
transcription coactivator binding [PMID:15620355]"
/note="Regulator of ribosomal protein (RP) transcription;
has forkhead associated domain that binds phosphorylated
proteins; recruits coactivator Ifh1p or corepressor Crf1p
to RP gene promoters; also has forkhead DNA-binding domain
though in vitro DNA binding assays give inconsistent
results; computational analyses suggest it binds DNA
directly at highly active RP genes and indirectly through
Rap1p motifs at others; suppresses RNA pol III and
splicing factor prp4 mutants"
/codon_start=1
/product="Fhl1p"
/protein_id="NP_015429.1"
/db_xref="GeneID:856219"
/db_xref="SGD:S000006308"
/translation="MDGEMAIIESSNHVGTSSPTTETQFTIDSSALKDQETKESITNS
PTSEVPIETKLPKSSDIVTEEKHPQNTTTDIENEVENPVTDDNGNLKLELPDNLDNAD
FSKLLEFDAKNDEALFNSNELLSHTMDPVNNIDLTHDHSREVSSKEDINIEPVNPDED
EREKTQDNTAAVKTEGIRNSEDTSIQKDEPTADAIYTDVHKLSVNKDTETLPTLVDEK
NNMLHMRNNSITPIMFQQHELVGQPPQNTVTENNSTDAETTQRKLSEPIDASLPLPNE
QPTIFAYARLDFQSFTFYVQTLHAIIGRRSENDFSHKVDVNLGPSKSISRRHAQIFYN
FGTGRFELSIIGKNGAFVDDIFVEKGNTVPLRNKTKIQIGQIPFQFILPEQERNDDSK
SPENADIAESEINTRNLKKNEPKSKKKITTGAKPKKAQTKPAVKKEKKPPKIPKKVYT
LEEIPVEYRTKPTVSYSAMLTTCIRKYSTAKGMSLSEIYAGIRELFPYYKYCPDGWQS
SVRHNLSLNKSFRKVSKEGKGWLWGLDEEYIAERERQKKKQSEIAVAKAQAAQLKLEQ
QQHKLQQVPQRGKKDIVSQRSNVNARKQNISQTLAANRAASNRKNTASDNQRTMKYLQ
EQLVILTRDRKGLSKQVIAAILTQALAMTINQVTQAAKNKGITGNPLTALMDKNPQHL
NLILAAAVNAATAKVTKGEVKQLVNPETTAAAALAAKAQHSKPIRQPIVQTPHVPDRP
PSQLSASASSHPNNYLHDKQPGSFDPSSLSRFFQPRQNARATSSVAATSVPAAASQNV
DAQPKPKPAQDNDLESESGTSSSSSSSSESGSESDSGSDDGSASGSGDNSSTSSESES
ESDSGSEVDEKNNKNEKIDSESIKNNESKDDIPSKDENSSNDNREISKTDEEGHDSKR
RKVSEDINEGITEVNVSLEEKL"
gene complement(<736984..>739569)
/gene="COG4"
/locus_tag="YPR105C"
/gene_synonym="COD1; SEC38; SGF1"
/db_xref="GeneID:856220"
mRNA complement(<736984..>739569)
/gene="COG4"
/locus_tag="YPR105C"
/gene_synonym="COD1; SEC38; SGF1"
/product="Golgi transport complex subunit COG4"
/transcript_id="NM_001184202.1"
/db_xref="GeneID:856220"
CDS complement(736984..739569)
/gene="COG4"
/locus_tag="YPR105C"
/gene_synonym="COD1; SEC38; SGF1"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0000301
retrograde transport, vesicle recycling within Golgi
[PMID:11703943]"
/experiment="EXISTENCE:mutant phenotype:GO:0000425
pexophagy [PMID:20065092]"
/experiment="EXISTENCE:mutant phenotype:GO:0006891
intra-Golgi vesicle-mediated transport [PMID:11703943]"
/experiment="EXISTENCE:mutant phenotype:GO:0016236
macroautophagy [PMID:20065092]"
/experiment="EXISTENCE:mutant phenotype:GO:0017119 Golgi
transport complex [PMID:11703943]"
/experiment="EXISTENCE:mutant phenotype:GO:0032258
cytoplasm to vacuole targeting by the Cvt pathway
[PMID:20065092]"
/note="Essential component of the conserved oligomeric
Golgi complex; a cytosolic tethering complex (Cog1p
through Cog8p) that functions in protein trafficking to
mediate fusion of transport vesicles to Golgi
compartments"
/codon_start=1
/product="Golgi transport complex subunit COG4"
/protein_id="NP_015430.1"
/db_xref="GeneID:856220"
/db_xref="SGD:S000006309"
/translation="MEGQKSNASWESSIIEGQLSKNLARYTLLLDKLSTLSQIDKLSE
VIANDYAKQSKQLNAFVQQSQSSLNKESRKLELQRTNLTTTLTQFHETVATISSSNAR
AKAIHDDIETVDQERALVNKTLQFVKDVRTLKNNISLAHSALETKDYLVAATAINEIR
SLPDKKLIVSEFAKKVVPSSEIPEEPAILIKNWCKELTSLFQEQFMEATRTQDIKELT
LMFKMFPMIGQDVLGLDLYSKYVCDIIADESRKIMSNSMENSTKFQGFFSQVLLHLFK
IVSTIINDHSKVIATCYGKKHMVHVMEKVEKEADLQASLILDIFMETRKIERTIHDIN
EWEHSQKNEDVNIDSNQSDIETDGETEKSSIISIHDLALLIMEFSQILQNWSMYSRFY
SVKWNEFSDLHPHVLQPPPPIADGKFALKLKQDKVFDEFQVFVLNHLQRSFRNSISLE
ELPSLNDLITAVPLNDHDNISYPVTSVLDDLILLVRKNLISVVNTGQFKLLASFLNEL
VKFFQNRFLVKFMQNKFKLLQSKLASNVSLKRYIPKGEEQSATSRSVSPPANKFSPLS
RFTFRGAAASALTNIQSNLQAVVAEDEDSILALHHYLIYLNTLYLSKVYVHRLLSIEI
LEDDSQRILRDNFPFDNDAAQLQNLIINSEKLVLEQTDKLSKWAVKYLFQNILQNRVR
NLLGTVFVNSASSNSSTSNQKNVSRDYSAGSNQKNYITSIEDFEDLSQINSFNSKWNQ
LIIPYKNILHNEAYAELLSVIVDYIVTTLEQRIWTLEFNELGVTKLDRELSLFIGNMC
GLNYNLREKFLKLTQIVLLLGLDDDNFDLTTGDIKDDFNGTFDWVINSQERIKARNMK
IDRTQ"
gene <740061..>741392
/gene="ISR1"
/locus_tag="YPR106W"
/db_xref="GeneID:856221"
mRNA <740061..>741392
/gene="ISR1"
/locus_tag="YPR106W"
/product="putative protein kinase ISR1"
/transcript_id="NM_001184203.1"
/db_xref="GeneID:856221"
CDS 740061..741392
/gene="ISR1"
/locus_tag="YPR106W"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:genetic interaction:GO:0018105
peptidyl-serine phosphorylation [PMID:32579556]"
/experiment="EXISTENCE:genetic interaction:GO:0018107
peptidyl-threonine phosphorylation [PMID:32579556]"
/experiment="EXISTENCE:genetic interaction:GO:0106279
negative regulation of UDP-N-acetylglucosamine
biosynthetic process [PMID:32579556]"
/experiment="EXISTENCE:mutant phenotype:GO:0018105
peptidyl-serine phosphorylation [PMID:32579556]"
/experiment="EXISTENCE:mutant phenotype:GO:0018107
peptidyl-threonine phosphorylation [PMID:32579556]"
/experiment="EXISTENCE:mutant phenotype:GO:0106279
negative regulation of UDP-N-acetylglucosamine
biosynthetic process [PMID:32579556]"
/note="Predicted protein kinase; negatively regulates the
hexosamine biosynthetic pathway (HBP), which converts
fructose-6-phosphate into UDP-N-acetylglucosamine, a
precursor of chitin biosynthesis, GPI-anchor formation and
glycosylation; interacts genetically with GFA1, encoding
the first enzyme in the HBP; involved in Gfa1p
phosphorylation (S332, T334); contains a Pho85p-regulated
SCF-CDC4 phosphodegron; unstable, cell cycle regulated
protein, peaking at G1/S"
/codon_start=1
/product="putative protein kinase ISR1"
/protein_id="NP_015431.1"
/db_xref="GeneID:856221"
/db_xref="SGD:S000006310"
/translation="MNSTPPTSPVTRVSDGSFPSISNNSKGFAYRQPQKHKSNFAYSH
LVSPVEEPTAKFSEAFQTDYSSKAPVATSEAHLKNDLDVLFTTPRFYSPENLALMFRL
SNTVSSLEFLDEFLMGILLAPEMDFLSNPSYSLPSNKLVGQGSYSYVYPISSSASSRC
NNDSGVVLKFAKSQHKSKVILQEALTLAYLQYMSPSTNESHIIPFYGLTYITKSHFRR
LRSNECVPGLILPKCEMSLYHFNTAVSHKLSLITKRKIWWRLMKQMIDALKSLKTNGI
IHGDIKTANILITEMHVLNGGHCKDFDFYLADFTSAFHINQTPTDLNTTVEYCAPELI
DSSSDHVPTFESDLYAVGLCLLSFISQHEPYNELQALVSHGSSPGIGSSSIQQSQWLI
NALLKKDPINLNMLRNDLFQDWKSELALLSRILVDRLPLENLITILDSNYI"
gene complement(<741438..>742064)
/gene="YTH1"
/locus_tag="YPR107C"
/db_xref="GeneID:856222"
mRNA complement(<741438..>742064)
/gene="YTH1"
/locus_tag="YPR107C"
/product="cleavage polyadenylation factor RNA-binding
subunit YTH1"
/transcript_id="NM_001184204.1"
/db_xref="GeneID:856222"
CDS complement(741438..742064)
/gene="YTH1"
/locus_tag="YPR107C"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:9224719]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005847 mRNA
cleavage and polyadenylation specificity factor complex
[PMID:12819204]"
/experiment="EXISTENCE:direct assay:GO:0006397 mRNA
processing [PMID:11344258]"
/experiment="EXISTENCE:mutant phenotype:GO:0006397 mRNA
processing [PMID:10899131]"
/note="Essential RNA-binding component of cleavage and
polyadenylation factor; contains five zinc fingers;
required for pre-mRNA 3'-end processing and
polyadenylation; relocalizes to the cytosol in response to
hypoxia"
/codon_start=1
/product="cleavage polyadenylation factor RNA-binding
subunit YTH1"
/protein_id="NP_015432.1"
/db_xref="GeneID:856222"
/db_xref="SGD:S000006311"
/translation="MSLIHPDTAKYPFKFEPFLRQEYSFSLDPDRPICEFYNSREGPK
SCPRGPLCPKKHVLPIFQNKIVCRHWLRGLCKKNDQCEYLHEYNLRKMPECVFFSKNG
YCTQSPDCQYLHIDPASKIPKCENYEMGFCPLGSSCPRRHIKKVFCQRYMTGFCPLGK
DECDMEHPQFIIPDEGSKLRIKRDDEINTRKMDEEKERRLNAIINGEV"
gene <742454..>743743
/gene="RPN7"
/locus_tag="YPR108W"
/db_xref="GeneID:856223"
mRNA <742454..>743743
/gene="RPN7"
/locus_tag="YPR108W"
/product="proteasome regulatory particle lid subunit RPN7"
/transcript_id="NM_001184205.1"
/db_xref="GeneID:856223"
CDS 742454..743743
/gene="RPN7"
/locus_tag="YPR108W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:19446323]"
/experiment="EXISTENCE:direct assay:GO:0008541 proteasome
regulatory particle, lid subcomplex [PMID:9741626]"
/experiment="EXISTENCE:mutant phenotype:GO:0005198
structural molecule activity [PMID:15102831]"
/note="Essential non-ATPase regulatory subunit of the 26S
proteasome; similar to another S. cerevisiae regulatory
subunit, Rpn5p, as well as to mammalian proteasome
subunits"
/codon_start=1
/product="proteasome regulatory particle lid subunit RPN7"
/protein_id="NP_015433.1"
/db_xref="GeneID:856223"
/db_xref="SGD:S000006312"
/translation="MVDVEEKSQEVEYVDPTVNRVPNYEVSEKAFLLTQSKVSIEQRK
EAAEFVLAKIKEEEMAPYYKYLCEEYLVNNGQSDLEHDEKSDSLNEWIKFDQELYNEL
CKKNESKIKELNEKIQKLEEDDEGELEQAQAWINLGEYYAQIGDKDNAEKTLGKSLSK
AISTGAKIDVMLTIARLGFFYNDQLYVKEKLEAVNSMIEKGGDWERRNRYKTYYGIHC
LAVRNFKEAAKLLVDSLATFTSIELTSYESIATYASVTGLFTLERTDLKSKVIDSPEL
LSLISTTAALQSISSLTISLYASDYASYFPYLLETYANVLIPCKYLNRHADFFVREMR
RKVYAQLLESYKTLSLKSMASAFGVSVAFLDNDLGKFIPNKQLNCVIDRVNGIVETNR
PDNKNAQYHLLVKQGDGLLTKLQKYGAAVRLTGSDRV"
gene 744284..744355
/locus_tag="YNCP0016W"
/db_xref="GeneID:856224"
tRNA 744284..744355
/locus_tag="YNCP0016W"
/product="tRNA-Thr"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0030371 translation
repressor activity [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043555 regulation
of translation in response to stress [PMID:27609601]"
/note="Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:856224"
/db_xref="SGD:S000006752"
gene <744689..>745573
/gene="GLD1"
/locus_tag="YPR109W"
/db_xref="GeneID:856225"
mRNA <744689..>745573
/gene="GLD1"
/locus_tag="YPR109W"
/product="Gld1p"
/transcript_id="NM_001184206.1"
/db_xref="GeneID:856225"
CDS 744689..745573
/gene="GLD1"
/locus_tag="YPR109W"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:29355480]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005802 trans-Golgi
network [PMID:29355480]"
/experiment="EXISTENCE:direct assay:GO:0044695 Dsc E3
ubiquitin ligase complex [PMID:29355480]"
/experiment="EXISTENCE:mutant phenotype:GO:0031503
protein-containing complex localization [PMID:29355480]"
/experiment="EXISTENCE:mutant phenotype:GO:0140624 EGAD
pathway [PMID:31368600]"
/note="Trafficking adaptor for the Dsc E3 ligase complex;
component of a Dsc subcomplex that sorts the E3 ligase to
the Golgi and to endosomes; involved in the endosome and
Golgi-associated degradation pathway (EGAD), contributing
to proteostasis and lipid homeostasis; SWAT-GFP and
mCherry fusion proteins localize to the endoplasmic
reticulum; diploid deletion strain has high budding index"
/codon_start=1
/product="Gld1p"
/protein_id="NP_015434.1"
/db_xref="GeneID:856225"
/db_xref="SGD:S000006313"
/translation="MDNFESTAEGNLSIGNKRVYELRKRNFQRNLVNNLSFLGYVLIS
LEYIKYDRTVWTLITRAIVQSLISSPFPSDAKLRRLATLGADNNTTGVATLPGGRSIR
FPGMFGTEMLYNSSSEAEQQDHDDTAIVSMKKQIRKFLFHGCLSLNMLFIILTILFPI
DFFEPLSGSEPVDDGPKNTPSPFSNSDGLLLGERRGGLFLQMIGERLPKSNFSGNLGL
VMFEFSILIVQFTLFSLTCVVLADLDFEEPERLEPVNSDGYDGSVIVARIPLNKTMNA
ILNDGNINDNNENASNSV"
gene complement(<745828..>746835)
/gene="RPC40"
/locus_tag="YPR110C"
/gene_synonym="RPC5"
/db_xref="GeneID:856226"
mRNA complement(<745828..>746835)
/gene="RPC40"
/locus_tag="YPR110C"
/gene_synonym="RPC5"
/product="DNA-directed RNA polymerase core subunit RPC40"
/transcript_id="NM_001184207.1"
/db_xref="GeneID:856226"
CDS complement(745828..746835)
/gene="RPC40"
/locus_tag="YPR110C"
/gene_synonym="RPC5"
/experiment="EXISTENCE:direct assay:GO:0003899
DNA-directed RNA polymerase activity
[PMID:9837969|PMID:3905793]"
/experiment="EXISTENCE:direct assay:GO:0005666 RNA
polymerase III complex [PMID:10611227|PMID:3905793]"
/experiment="EXISTENCE:direct assay:GO:0005736 RNA
polymerase I complex [PMID:11486042|PMID:9837969]"
/experiment="EXISTENCE:direct assay:GO:0006360
transcription by RNA polymerase I [PMID:9837969]"
/experiment="EXISTENCE:direct assay:GO:0042797 tRNA
transcription by RNA polymerase III [PMID:3905793]"
/note="RNA polymerase subunit AC40; common to RNA
polymerase I and III; predominant determinant targeting
Ty1 integration upstream of Pol III-transcribed genes"
/codon_start=1
/product="DNA-directed RNA polymerase core subunit RPC40"
/protein_id="NP_015435.1"
/db_xref="GeneID:856226"
/db_xref="SGD:S000006314"
/translation="MSNIVGIEYNRVTNTTSTDFPGFSKDAENEWNVEKFKKDFEVNI
SSLDAREANFDLINIDTSIANAFRRIMISEVPSVAAEYVYFFNNTSVIQDEVLAHRIG
LVPLKVDPDMLTWVDSNLPDDEKFTDENTIVLSLNVKCTRNPDAPKGSTDPKELYNNA
HVYARDLKFEPQGRQSTTFADCPVVPADPDILLAKLRPGQEISLKAHCILGIGGDHAK
FSPVSTASYRLLPQINILQPIKGESARRFQKCFPPGVIGIDEGSDEAYVKDARKDTVS
REVLRYEEFADKVKLGRVRNHFIFNVESAGAMTPEEIFFKSVRILKNKAEYLKNCPIT
Q"
gene <747306..>749000
/gene="DBF20"
/locus_tag="YPR111W"
/db_xref="GeneID:856227"
mRNA <747306..>749000
/gene="DBF20"
/locus_tag="YPR111W"
/product="serine/threonine-protein kinase DBF20"
/transcript_id="NM_001184208.1"
/db_xref="GeneID:856227"
CDS 747306..749000
/gene="DBF20"
/locus_tag="YPR111W"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004674 protein
serine/threonine kinase activity [PMID:8131744]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005816 spindle
pole body [PMID:12937277]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:12937277]"
/experiment="EXISTENCE:genetic interaction:GO:0000281
mitotic cytokinesis [PMID:12937277]"
/experiment="EXISTENCE:genetic interaction:GO:0010458 exit
from mitosis [PMID:1916278]"
/experiment="EXISTENCE:mutant phenotype:GO:0000917
division septum assembly [PMID:17005296]"
/experiment="EXISTENCE:mutant phenotype:GO:0061013
regulation of mRNA catabolic process [PMID:22196726]"
/note="Ser/Thr kinase involved in late nuclear division;
one of the mitotic exit network (MEN) proteins; necessary
for the execution of cytokinesis; also plays a role in
regulating the stability of SWI5 and CLB2 mRNAs; DBF20 has
a paralog, DBF2, that arose from the whole genome
duplication"
/codon_start=1
/product="serine/threonine-protein kinase DBF20"
/protein_id="NP_015436.1"
/db_xref="GeneID:856227"
/db_xref="SGD:S000006315"
/translation="MFSRSDREVDDLAGNMSHLGFYDLNIPKPTSPQAQYRPARKSEN
GRLTPGLPRSYKPCDSDDQDTFKNRISLNHSPKKLPKDFHERASQSKTQRVVNVCQLY
FLDYYCDMFDYVISRRQRTKQVLRYLEQQRSVKNVSNKVLNEEWALYLQREHEVLRKR
RLKPKHKDFQILTQVGQGGYGQVYLAKKKDSDEICALKILNKKLLFKLNETNHVLTER
DILTTTRSDWLVKLLYAFQDPESLYLAMEFVPGGDFRTLLINTRILKSGHARFYISEM
FCAVNALHELGYTHRDLKPENFLIDATGHIKLTDFGLAAGTVSNERIESMKIRLEEVK
NLEFPAFTERSIEDRRKIYHNMRKTEINYANSMVGSPDYMALEVLEGKKYDFTVDYWS
LGCMLFESLVGYTPFSGSSTNETYENLRYWKKTLRRPRTEDRRAAFSDRTWDLITRLI
ADPINRVRSFEQVRKMSYFAEINFETLRTSSPPFIPQLDDETDAGYFDDFTNEEDMAK
YADVFKRQNKLSAMVDDSAVDSKLVGFTFRHRDGKQGSSGILYNGSEHSDPFSTFY"
rep_origin 749099..749345
/note="ARS1626; Autonomously Replicating Sequence"
/db_xref="SGD:S000118422"
gene complement(<749256..>751919)
/gene="MRD1"
/locus_tag="YPR112C"
/db_xref="GeneID:856228"
mRNA complement(<749256..>751919)
/gene="MRD1"
/locus_tag="YPR112C"
/product="RNA-binding ribosome biosynthesis protein MRD1"
/transcript_id="NM_001184209.1"
/db_xref="GeneID:856228"
CDS complement(749256..751919)
/gene="MRD1"
/locus_tag="YPR112C"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:11884397]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911|PMID:23193268]"
/experiment="EXISTENCE:direct assay:GO:0042134 rRNA
primary transcript binding [PMID:23193268]"
/experiment="EXISTENCE:mutant phenotype:GO:0000447
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from
5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11884397]"
/experiment="EXISTENCE:mutant phenotype:GO:0000472
endonucleolytic cleavage to generate mature 5'-end of
SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:11884397]"
/experiment="EXISTENCE:mutant phenotype:GO:0000480
endonucleolytic cleavage in 5'-ETS of tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:11884397]"
/experiment="EXISTENCE:mutant phenotype:GO:0034462
small-subunit processome assembly [PMID:18586827]"
/note="Essential conserved small ribosomal subunit (40s)
synthesis factor; component of the 90S preribosome;
required for production of 18S rRNA and small ribosomal
subunit; contains five consensus RNA-binding domains and
binds to the pre-rRNA at two sites within the 18S region"
/codon_start=1
/product="RNA-binding ribosome biosynthesis protein MRD1"
/protein_id="NP_015437.1"
/db_xref="GeneID:856228"
/db_xref="SGD:S000006316"
/translation="MSRIIVKGLPVYLTDDNLREHFTKRLRQKHSHQAVNGSGPDLIT
DVKILRDRNGESRRFGFIGYRNEEDAFDAVEYFNGSFINTSKIEVSMAKSFADPRVPQ
PMKEKRREALKRFREKEEKLLQEENRKKKKVDENKHSNIDDEIRKNKQLQEFMETMKP
SSQVTSWEKVGIDKSIEDEKLKREEEDSSVQGNSLLAHALALKEENNKDEAPNLVIEN
ESDDEYSALNRNRDEDQEDAGEEEKMISISNLKDTDIGLVNDDANSDEKENEKRRNLA
QDEKVSDLDWFKQRRVRIKESEAETREKSSSYATEQNESLDTKKEEQPERAVPQKTDE
ELAIEKINQTGRLFLRNILYTSKEEDFRKLFSPFGELEEVHVALDTRTGQSKGFAYVL
FKDSKNAVNAYVELDKQIFQGRLLHILPGEEKKSHRLDEFDLKNMPLKKQKELKRKAA
ASRQTFSWNSLYMNQDAVLGSVAAKLGLEKSQLIDAENSSSAVKQALAEAHVIGDVRK
YFESKGVDLTKFSQLKSTNQRDDKVILVKNFPFGTTREELGEMFLPYGKLERLLMPPA
GTIAIVQFRDTTSARAAFTKLSYKRFKDGIIYLERGPKDCFTKPAEADDLINNTSAKE
EENPVEVKPSSNDLMEANKDVTEGSSNAHDEDVIDGPTVSIFIKNLNFSTTNQNLTDR
FKVFTGFVVAQVKTKPDPKHQGKTLSMGFGFVEFRTKEQANAVIAAMDGTVIDGHKIQ
LKLSHRQASQSGNTKTKSNKKSGKIIVKNLPFEATRKDVFELFNSFGQLKSVRVPKKF
DKSARGFAFVEFLLPKEAENAMDQLHGVHLLGRRLVMQYAEEDAVDAEEEIARMTKKV
RKQVATNEMAALRNGGGRKKLDVDDEENEGF"
gene <752257..>752919
/gene="PIS1"
/locus_tag="YPR113W"
/db_xref="GeneID:856229"
mRNA <752257..>752919
/gene="PIS1"
/locus_tag="YPR113W"
/product="CDP-diacylglycerol--inositol
3-phosphatidyltransferase"
/transcript_id="NM_001184210.1"
/db_xref="GeneID:856229"
CDS 752257..752919
/gene="PIS1"
/locus_tag="YPR113W"
/EC_number="2.7.8.11"
/experiment="EXISTENCE:direct assay:GO:0003881
CDP-diacylglycerol-inositol 3-phosphatidyltransferase
activity [PMID:3005284]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:3005242]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:8543066]"
/experiment="EXISTENCE:direct assay:GO:0006661
phosphatidylinositol biosynthetic process [PMID:3005284]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:6274497]"
/experiment="EXISTENCE:mutant phenotype:GO:0003881
CDP-diacylglycerol-inositol 3-phosphatidyltransferase
activity [PMID:6088233]"
/experiment="EXISTENCE:mutant phenotype:GO:0006661
phosphatidylinositol biosynthetic process [PMID:6088233]"
/note="Phosphatidylinositol synthase; required for
biosynthesis of phosphatidylinositol, which is a precursor
for polyphosphoinositides, sphingolipids, and glycolipid
anchors for some of the plasma membrane proteins"
/codon_start=1
/product="CDP-diacylglycerol--inositol
3-phosphatidyltransferase"
/protein_id="NP_015438.1"
/db_xref="GeneID:856229"
/db_xref="SGD:S000006317"
/translation="MSSNSTPEKVTAEHVLWYIPNKIGYVRVITAALSFFVMKNHPTA
FTWLYSTSCLLDALDGTMARKYNQVSSLGAVLDMVTDRSSTAGLMCFLCVQYPQWCVF
FQLMLGLDITSHYMHMYASLSAGKTSHKSVGEGESRLLHLYYTRRDVLFTICAFNELF
YAGLYLQLFSNSATFGKWTTIISFPGYVFKQTANVVQLKRAALILADNDAKNANEKNK
TY"
gene <753301..>754248
/gene="ALE2"
/locus_tag="YPR114W"
/db_xref="GeneID:856230"
mRNA <753301..>754248
/gene="ALE2"
/locus_tag="YPR114W"
/product="Ale2p"
/transcript_id="NM_001184211.1"
/db_xref="GeneID:856230"
CDS 753301..754248
/gene="ALE2"
/locus_tag="YPR114W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0071618
lysophosphatidylethanolamine acyltransferase activity
[PMID:39970228]"
/experiment="EXISTENCE:mutant phenotype:GO:0036152
phosphatidylethanolamine acyl-chain remodeling
[PMID:39970228]"
/note="Lysophosphatidylethanolamine acyltransferase that
regulates phospholipid composition; involved in remodeling
membrane phospholipid acyl chains; TRAM-LAG1-CLN8
domain-containing (TLCD) protein family member; homolog of
human TLCD1; distant similarity to yeast ceramide
synthases, Lag1p and Lac1p; mutation confers sensitivity
to inhibitors of sphingolipid biosynthesis"
/codon_start=1
/product="Ale2p"
/protein_id="NP_015439.1"
/db_xref="GeneID:856230"
/db_xref="SGD:S000006318"
/translation="MDVLLSLPQPELFKTTVIPFLANRNIIKSEAILSNLHSIFYVAI
FYHIWFLFGKWILFPHLVKWKLDYDQKHNVKKDEKTTSERQAQHYKKKYTSLINQSSV
HLISLLQSIVVLYYSLKFLLDPKASAEPYQTSHSRVFTENRDTQVICIFAIGYFVWDI
YISTMYSTFPFVVHGIISTVVFCIGLKPYIQYYAPVFLMFELSNPSLNFRWFGIKFLP
QKSKFCSLLLLLNNLTLMVVFFAARIAWGWFQIGKLCYDFYQVRNEPGFLVFDTIVIL
AGNFVLDILNVIWFSTMVSVAAKVLKKGESVDKVTKNEQ"
gene <754877..>758128
/gene="RGC1"
/locus_tag="YPR115W"
/db_xref="GeneID:856231"
mRNA <754877..>758128
/gene="RGC1"
/locus_tag="YPR115W"
/product="Rgc1p"
/transcript_id="NM_001184212.1"
/db_xref="GeneID:856231"
CDS 754877..758128
/gene="RGC1"
/locus_tag="YPR115W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:11914276]"
/experiment="EXISTENCE:mutant phenotype:GO:0090372
positive regulation of glycerol transport [PMID:19956799]"
/note="Putative regulator of the Fps1p glycerol channel;
multiply phosphorylated by Hog1p under osmotic stress;
contains a pleckstrin homology domain; forms homodimers
and heterodimerizes with paralog Ask10p; green fluorescent
protein (GFP)-fusion protein localizes to the cytoplasm"
/codon_start=1
/product="Rgc1p"
/protein_id="NP_015440.1"
/db_xref="GeneID:856231"
/db_xref="SGD:S000006319"
/translation="MSDYFTFPKQENGGISKQPATPGSTRSSSRNLELPKNYRSFGGS
SDELASMYSADSQYLMDMIPDSLTLKNEPASGNTQMNGPDGKENKDIKLDEYILPKTD
PRSPYYINMPIPKKLPKSEGKARAKQKVNRADPSDLDVENIYETSGEFVREYPTDILI
DRFHKWKKILKSLIAYFREAAYSQEQIARINYQMKNAVKFAFLTDLEDETNKLVDPSI
SKLPTKKPQPVPLAAQKLDSKYDTDVEQPQSIQSVPSEEVASASSGFMKFGSGSIQDI
QVILKKYHLSLGSQQYKISKEILAYIIPKLTDLRKDLTTKMKEIKELNGDFKTNIGEH
IKITSRLLNKYIASVKLLDEASTSGDKQGEKLKPKHDPYLLKLQLDLQLKRQLLEENY
LREAFLNLQSAALQLEKIVYSKIQSALQRYSALIDSEARLMIKNLCHELQQGILSRPP
AVEWDNFVSHHPTCLMNLKSTDPPPQPRRLSDIVYPNMKSPLAKCIRVGYLLKKTESS
KSFTKGYFVLTTNYLHEFKSSDFFLDSKSPRSKNKPVVEQSDISRVNKDGTNAGSHPS
SKGTQDPKLTKRRKGLSSSNLYPISSLSLNDCSLKDSTDSTFVLQGYASYHSPEDTCT
KESSTTSDLACPTKTLASNKGKHQRTPSALSMVSVPKFLKSSSVPKEQKKAKEEANIN
KKSICEKRVEWTFKIFSASLEPTPEESKNFKKWVQDIKALTSFNSTQERSNFIEEKIL
KSRNHNNGKSSQRSKNSTYITPVDSFVNLSEKVTPSSSVTTLNTRKRANRPRYIDIPK
SANMNAGAMNSVYRSKVNTPAIDENGNLAIVGETKNSAPQNGMSYTIRTPCKSPYSPY
TGEGMLYNRSADNLMASSSRKASAPGEVPQIAVSNHGDEAIIPASAYSDSSHKSSRAS
SVASIHNQRVDFYPSPLMNLPGVSPSCLALDGNANGYFGIPLNCNSEARRGSDLSPFE
MESPLFEENRTQNCSGSRKSSACHIPHQCGPRKEGNDSRLIYGNEKGASQSRLTLKEP
LTSKGVEAPYSSLKKTYSAENVPLTSTVSNDKSLHSRKEGSTNTVPATSASSK"
gene <758648..>759481
/gene="RRG8"
/locus_tag="YPR116W"
/gene_synonym="MTA1"
/db_xref="GeneID:856232"
mRNA <758648..>759481
/gene="RRG8"
/locus_tag="YPR116W"
/gene_synonym="MTA1"
/product="Rrg8p"
/transcript_id="NM_001184213.1"
/db_xref="GeneID:856232"
CDS 758648..759481
/gene="RRG8"
/locus_tag="YPR116W"
/gene_synonym="MTA1"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0099617 matrix side
of mitochondrial inner membrane [PMID:30759361]"
/experiment="EXISTENCE:mutant phenotype:GO:0097745
mitochondrial tRNA 5'-end processing [PMID:30759361]"
/note="hypothetical protein; required for efficient 5'
processing of mitochondrial tRNAs, for respiratory growth
and mitochondrial genome maintenance; null mutation
results in a decrease in plasma membrane electron
transport; localizes to the matrix side of the inner
mitochondrial membrane"
/codon_start=1
/product="Rrg8p"
/protein_id="NP_015441.1"
/db_xref="GeneID:856232"
/db_xref="SGD:S000006320"
/translation="MGLPKSAYKKLLIDCPTRVINKNCAQRVKDVSPLITNFEKWSDK
RKKLYFKDEEEMVGQFHLENFNLKNNLYGRLLASPMRAEKISKLKSCRELLIPLKVVP
STGKDQHADKDKLKLVPTLDYSKSYKSSYVLNSASIVQDNLAAATSWFPISVLQTSTP
KSLEVDSSTFITEYNANLHAFIKARLSVIPNVGPSSINRVLLICDKRKTPPIEIQVVS
HGKGLPITQSVFNLGYLHEPTLEAIVSKDAVTNGIYLDADNDKDLIKHLYSTLLFHSV
N"
gene <760025..>767494
/gene="HOB2"
/locus_tag="YPR117W"
/db_xref="GeneID:856233"
mRNA <760025..>767494
/gene="HOB2"
/locus_tag="YPR117W"
/product="Hob2p"
/transcript_id="NM_001184214.1"
/db_xref="GeneID:856233"
CDS 760025..767494
/gene="HOB2"
/locus_tag="YPR117W"
/note="Modulator of cellular lipid distribution; localizes
to ER-plasma membrane and ER-mitochondria contact sites;
shares homology with lipid transfer proteins Vps13p and
Atg2p; ortholog of D. melanogaster Hobbit protein"
/codon_start=1
/product="Hob2p"
/protein_id="NP_015442.1"
/db_xref="GeneID:856233"
/db_xref="SGD:S000006321"
/translation="MSMLPWSQIRDVSKLLLGFMLFIISIQKIASILMSWILMLRHST
IRKISFGYFFGTSIRRAFILTDFAQIYIGKITLRIGWKPGIVFHNVDLKLFGKDSHIT
AHSTKDSRTYFNPRDQTFTFVINRRVLSILKLVFSFSTFFHTLALTVPNGKQYKLNIG
SITISHPHDDTIKLEAFLHDFTHPETKDTLNHTGFFMVCKIGKEDDTGSNCTKVILKN
WKSSLKISDVCWHLPEKKGKNLHSEPVEPFSAGDDAEMLTSYRKMLKPFHYPLKTLNI
LDLKVENVKLIYKKKFTIRISSAQLYLESISILNNVSALELLPLNKPTWGDFELSLSA
NAVVVDIDGNTAVRIPFGNVILTSDILLFLLDNVPLRRTKVSSILNIINPSVFLTIHQ
VLEVLHLVDKFDSPETSSCTNTNDRSLNILDLDIDRLPSFNFELLMSNFISRLHISDE
ENVTFKVFSTHALFSRNNLSMTPKKGQVMQIRPDWPFAKTALVSDQLSNYIKIVGTSL
SYLRIPTEQDANPVSIPVCGFERLDTFLDEFSNSKLIVQSTLRHSYVSLENIEVLHTL
SRAFDKIYLLISSRTKRNAAHKANGGKLGDLNEAKKTFNWSLKLRMKDISCSLLVAGF
LPKNLDPVEAENFNLSDVTRGAKVVFTESILLADSQEKNFTIIDASVYRFMDGTTYKP
SPEVIIQFTNLLLSFNDSDEIHFSLPKIKFKMDVNIIWLWFYIRSIWIKFRPNSKLSR
NSVSSVKSVNVLDRLRVDIGKMIIELTLPHNTEVLLIFERIGLSSSTKNLTIASLSAY
VVSVYVKHIKVYVSLLNINDFELDTEELICKKSAVINTSLIHFHAEYHFRFYMITDNI
VTLYKSFKQIKLAFSNLNEFKRLYPQQQFPKKVPNLHICCQDFLIDIEEDPFEQELGL
ILKVGVLEQRERLKKLEEFKEKLSTYEDMNVRLRSLYDTSRGQSFFPEFYANDQEYEQ
KAYLRLLENFSTSWIARYRKAKLSFYGMPYRVISREELGTKYHLFTRQKTSTVANLVV
KDLDFKLGSPSFPLDNYMDFVYQYGKKVPKSTEYTLLIILGLKIKSALWELRLRDYPI
PAISFPDTFTTGDVVFAEKMPAPCALHTVYVPFVSSAQRSPYNDANTIYGLHIIRTIN
SVKTYFNIRSMVTSSSSARITWGKSLQPGYESLMLWFDFLTKPLIDPSKKLGFWDKFR
YLVHGKWIYEFSEESEIHLNIKGSHDPYKITDDGAGLAFCWSGGTTIYVHNSTDPKEF
LKIESQRFQLAVPDFAKVSKFDKVFMKLDGRVIWTLGLLFEQGDISKAGDEERFLPNR
PHYEIQLMNPDGVADLDHHDTYKGFRTSFIHMSFGVYSSEHGSINSLYLAPYALTHFF
KWWNLFHTYTSGPIRQGRLFTDVLQNKTKFGRSLFTIAYQLHLKRLMVTHIYRHITTQ
YDLEKDRKITFTGLKGRFDSLKIDLHQKRVKLTHTNQKLNKSKPVWKFKMSRGEIDCA
EADIRILSTLFDQEAVKEILTSGLDGILEDEPSRPITPQDVEYLRESDWYDYEDYIDL
NQVPLGSSLPLKLEAIPLLYSPRISYFRKINDDGYVLAYPFGTEESHNCLIGKNHPEL
TQEKLATERKREIEEQLKLLHITLSELQSNKGGGSVSGNSERYARELKAEVAELNHRL
HTVNTILSDLKISETIPGGNTDGDSSSSLSDTDVNLENAPPIQNRISLLRTNTVESFV
SMRKASTMQVESTYDNRFMVHNIELKIDNKIRHHLLEYASSAFERKSMRFAVTYKSVT
ILKELLGNVLTGVRTSVEDYGSILEDDLASNSEFIEHFEKLIREVPSDDFDYVDNYLF
RLISPQVQIKSDVERNAAVILAARDIEMGIIDIVQVYGKSGKRIPVDVDTIVETRYSA
VSKDIQLFTLFKKDLEGPEGRFFHKNGYGSDKESDIWPPWIPLEMCFDGSLLDKHVFL
KRRSMFLTYVAPNPLFFSANDTSAFSYDSRFRIAFPGLVLTSDCQQYCAVYAIAEDLL
SFGSSLDEKVEKLSRILFTDEVRNNLENLDVSVVTALQERIKELYYTRAYLKLHEPRL
FMKSGQELTFDIQTSTLKLTLLMTAIKKTYDRMGSGNRVIQKRLRWQVGTDELIWELY
DESKTPFVTIGLGPSTFIRSETSDGTNSNKVSISSLQCFNQQENPVYTELLAPFYENS
SYNKNAPMVEIFWILGPSVGGISDLQDLIVSLQPLIFKMDHKTSEKLMNYLFPKIEQT
SIEPNSPELVPRSSTSSFFSSSPVLRHSLSNGSLSVYDAKDVDSWDLRSIQSKEGIKK
HKGDHRKLSASLFVQPDYNINEMVKRSGTFFNVKSIIIRKTLMSVCYKGSHSLLTDVN
NLIVRVPVLKYHNKLWSREEFFTALKRDVVRIVLQHLGNIIGNKFLPHKKENKKKTSM
EIHRLLSPDSQNRDNSHILEVEGHNSFYSSTHSSDIRSINSDETYNENDGNGVKPFYP
VTSEFSKNK"
gene <767752..>768987
/gene="MRI1"
/locus_tag="YPR118W"
/db_xref="GeneID:856234"
mRNA <767752..>768987
/gene="MRI1"
/locus_tag="YPR118W"
/product="S-methyl-5-thioribose-1-phosphate isomerase
MRI1"
/transcript_id="NM_001184215.1"
/db_xref="GeneID:856234"
CDS 767752..768987
/gene="MRI1"
/locus_tag="YPR118W"
/EC_number="5.3.1.23"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0046523
S-methyl-5-thioribose-1-phosphate isomerase activity
[PMID:15215245]"
/experiment="EXISTENCE:mutant phenotype:GO:0019509
L-methionine salvage from methylthioadenosine
[PMID:15215245|PMID:18625006]"
/note="5'-methylthioribose-1-phosphate isomerase;
catalyzes the isomerization of
5-methylthioribose-1-phosphate to
5-methylthioribulose-1-phosphate in the methionine salvage
pathway"
/codon_start=1
/product="S-methyl-5-thioribose-1-phosphate isomerase
MRI1"
/protein_id="NP_015443.1"
/db_xref="GeneID:856234"
/db_xref="SGD:S000006322"
/translation="MSLEAIVFDRSEPENVSVKVLDQLLLPYTTKYVPIHTIDDGYSV
IKSMQVRGAPAIAIVGSLSVLTEVQLIKHNPTSDVATLYSLVNWESTKTVLNKRLDFL
LSSRPTAVNLSNSLVEIKNILKSSSDLKAFDGSLYNYVCELIDEDLANNMKMGDNGAK
YLIDVLQKDGFKDEFAVLTICNTGSLATSGYGTALGVIRSLWKDSLAKTDKADSGLDN
EKCPRMGHVFPLETRPYNQGSRLTAYELVYDKIPSTLITDSSIAYRIRTSPIPIKAAF
VGADRIVRNGDTANKIGTLQLAVICKQFGIKFFVVAPKTTIDNVTETGDDIIVEERNP
EEFKVVTGTVINPENGSLILNESGEPITGKVGIAPLEINVWNPAFDITPHELIDGIIT
EEGVFTKNSSGEFQLESLF"
gene complement(769207..769302)
/locus_tag="YNCP0017C"
/db_xref="GeneID:856235"
tRNA complement(join(769207..769242,769266..769302))
/locus_tag="YNCP0017C"
/product="tRNA-Lys"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE
analysis; thiolation of uridine at wobble position (34)
requires Ncs6p"
/db_xref="GeneID:856235"
/db_xref="SGD:S000006635"
repeat_region 769319..769659
/note="Ty3 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007216"
gene <771653..>773128
/gene="CLB2"
/locus_tag="YPR119W"
/db_xref="GeneID:856236"
mRNA <771653..>773128
/gene="CLB2"
/locus_tag="YPR119W"
/product="B-type cyclin CLB2"
/transcript_id="NM_001184216.1"
/db_xref="GeneID:856236"
CDS 771653..773128
/gene="CLB2"
/locus_tag="YPR119W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11171327|PMID:12972503]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:12972503]"
/experiment="EXISTENCE:direct assay:GO:0005816 spindle
pole body [PMID:12972503]"
/experiment="EXISTENCE:direct assay:GO:0005819 spindle
[PMID:12972503]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:12972503]"
/experiment="EXISTENCE:direct assay:GO:0016538
cyclin-dependent protein serine/threonine kinase regulator
activity [PMID:1731251]"
/experiment="EXISTENCE:genetic interaction:GO:0010696
positive regulation of mitotic spindle pole body
separation [PMID:1387566]"
/experiment="EXISTENCE:mutant phenotype:GO:0000086 G2/M
transition of mitotic cell cycle [PMID:8455600]"
/experiment="EXISTENCE:mutant phenotype:GO:0005737
cytoplasm [PMID:11171327]"
/experiment="EXISTENCE:mutant phenotype:GO:0016538
cyclin-dependent protein serine/threonine kinase regulator
activity [PMID:1731251|PMID:1849457]"
/experiment="EXISTENCE:mutant phenotype:GO:0032888
regulation of mitotic spindle elongation [PMID:23468650]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:22918234]"
/note="B-type cyclin involved in cell cycle progression;
also involved in response to DNA damage; activates Cdc28p
to promote the transition from G2 to M phase; accumulates
during G2 and M, then targeted via a destruction box motif
for ubiquitin-mediated degradation by the proteasome; CLB2
has a paralog, CLB1, that arose from the whole genome
duplication"
/codon_start=1
/product="B-type cyclin CLB2"
/protein_id="NP_015444.1"
/db_xref="GeneID:856236"
/db_xref="SGD:S000006323"
/translation="MSNPIENTENSQNTSSSRFLRNVQRLALNNVTNTTFQKSNANNP
ALTNFKSTLNSVKKEGSRIPQFTRESVSRSTAAQEEKRTLKENGIQLPKNNLLDDKEN
QDPSSQQFGALTSIKEGRAELPANISLQESSSAKEIIQHDPLKGVGSSTEVVHNSVEN
EKLHPARSQLQVRNTESETDSGKKRPISTIVEQELPKKFKVCDENGKEEYEWEDLDAE
DVNDPFMVSEYVNDIFEYLHQLEVITLPKKEDLYQHRNIHQNRDILVNWLVKIHNKFG
LLPETLYLAINIMDRFLGKELVQLDKLQLVGTSCLFIASKYEEVYSPSIKHFASETDG
ACTEDEIKEGEKFILKTLKFNLNYPNPMNFLRRISKADDYDIQSRTLAKFLLEISLVD
FRFIGILPSLCAAAAMFMSRKMLGKGKWDGNLIHYSGGYTKEELAPVCHMIMDYLVSP
IVHDEFHRKYQSRRFMKASIISVQWALKVRKNGYDIMTLHE"
gene complement(<773875..>775182)
/gene="CLB5"
/locus_tag="YPR120C"
/db_xref="GeneID:856237"
mRNA complement(<773875..>775182)
/gene="CLB5"
/locus_tag="YPR120C"
/product="B-type cyclin CLB5"
/transcript_id="NM_001184217.1"
/db_xref="GeneID:856237"
CDS complement(773875..775182)
/gene="CLB5"
/locus_tag="YPR120C"
/experiment="EXISTENCE:direct assay:GO:0001932 regulation
of protein phosphorylation [PMID:7954792]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10848575]"
/experiment="EXISTENCE:direct assay:GO:0016538
cyclin-dependent protein serine/threonine kinase regulator
activity [PMID:7954792]"
/experiment="EXISTENCE:genetic interaction:GO:0000082 G1/S
transition of mitotic cell cycle [PMID:8253070]"
/experiment="EXISTENCE:genetic interaction:GO:0006279
premeiotic DNA replication [PMID:9732268]"
/experiment="EXISTENCE:genetic interaction:GO:0010696
positive regulation of mitotic spindle pole body
separation [PMID:16688214|PMID:11438663]"
/experiment="EXISTENCE:genetic interaction:GO:0045740
positive regulation of DNA replication [PMID:8319908]"
/experiment="EXISTENCE:genetic interaction:GO:1901673
regulation of mitotic spindle assembly [PMID:8319908]"
/experiment="EXISTENCE:mutant phenotype:GO:0000082 G1/S
transition of mitotic cell cycle
[PMID:8319908|PMID:8253070]"
/experiment="EXISTENCE:mutant phenotype:GO:0000086 G2/M
transition of mitotic cell cycle [PMID:8319908]"
/experiment="EXISTENCE:mutant phenotype:GO:0006279
premeiotic DNA replication [PMID:9732268]"
/experiment="EXISTENCE:mutant phenotype:GO:0045740
positive regulation of DNA replication
[PMID:9734354|PMID:8319908]"
/note="B-type cyclin involved in DNA replication during S
phase; activates Cdc28p to promote initiation of DNA
synthesis; functions in formation of mitotic spindles
along with Clb3p and Clb4p; most abundant during late G1
phase; CLB5 has a paralog, CLB6, that arose from the whole
genome duplication"
/codon_start=1
/product="B-type cyclin CLB5"
/protein_id="NP_015445.1"
/db_xref="GeneID:856237"
/db_xref="SGD:S000006324"
/translation="MGENHDHEQSIKRNSMIYNENERQLCNSNLKILQNKRALSKNDS
SSKQQVQDSKPRRALTDVPVNNNPLSQNKRIVAGSKAAKVRREENIRPIVSAVQKRQI
YNDRTAAEQEEEEEEEGEDDDAASIVNKKRRIDAEGVSEIVGWQDLDYVEKDDTAMVA
EYSAEIFAFLYRRELETLPSHNYLLDKTSKYYLRPSMRTILVDWLVEVHEKFQCYPET
LFLSINLMDRFLAKNKVTMNKLQLLAVTSLFIAAKFEEVNLPKLAEYAYITDGAASKN
DIKNAEMFMLTSLEFNIGWPNPLNFLRRISKADDYDPVNRNIGKFILEYAYCCHQFIH
LPPSTVSAMAMYIARRMTNRNKNELWNGTLQHYSGGIDPIHDEAFQSLCIDLVKDIAS
SKTHLDSLILKYKKPRYGSVYFQTFKWCTSEMHSNFQNLFNLK"
gene complement(775765..775836)
/locus_tag="YNCP0018C"
/db_xref="GeneID:856238"
tRNA complement(775765..775836)
/locus_tag="YNCP0018C"
/product="tRNA-Cys"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Cysteine tRNA (tRNA-Cys), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:856238"
/db_xref="SGD:S000006529"
repeat_region 776093..776413
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007211"
repeat_region complement(776667..776796)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007201"
rep_origin 776926..777156
/note="ARS1635; Autonomously Replicating Sequence"
/db_xref="SGD:S000119041"
repeat_region 777832..778154
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007212"
gene <778583..>780301
/gene="THI22"
/locus_tag="YPR121W"
/db_xref="GeneID:856239"
mRNA <778583..>780301
/gene="THI22"
/locus_tag="YPR121W"
/product="putative phosphomethylpyrimidine kinase"
/transcript_id="NM_001184218.2"
/db_xref="GeneID:856239"
CDS 778583..780301
/gene="THI22"
/locus_tag="YPR121W"
/experiment="EXISTENCE:expression pattern:GO:0009228
thiamine biosynthetic process [PMID:10383756]"
/note="Protein with similarity to hydroxymethylpyrimidine
phosphate kinases; member of a gene family with THI20 and
THI21; not required for thiamine biosynthesis; SWAT-GFP
and mCherry fusion proteins localize to the endoplasmic
reticulum and vacuole respectively"
/codon_start=1
/product="putative phosphomethylpyrimidine kinase"
/protein_id="NP_015446.2"
/db_xref="GeneID:856239"
/db_xref="SGD:S000006325"
/translation="MVIILLGLCTLGFPRTAFCPSIMTNSTVSINTPPPYLTLACNEK
LPTVMSIAGSDSSGGAGVEADIKTITAHRCYAMTCVTTLTAQTPVKVYGAQNIPKKMV
SQILDANLQDMKCNVIKTGMLTVDAIEVLHEKLLQLGENRPKLVIDPVLCAASDSSPT
GKDVVSLIIEKISPFADILTPNISDCFMLLGENREVSKLQDVLEIAKDLSRITNCSNI
LVKGGHIPCDDGKEKHITDVLYLGAEQKFITFKGQFVNTTRTHGAGCTLASAIASNLA
RGYSLSQSVYGGIEYVQNAIAIGCDVTKKAVKVGPINHVYAVEIPLEKMLTDECFTAS
DAVPKKPIEGSLDKIPGGSFFNYLINHPKVKPHWDAYVNHEFVKRVADGTLERKKFQF
FIEQDYLYLIDYVRVCCVTGSKSPTLEDLEKDLVIADCARNELNEHERRLREEFGVKD
PDYLQKIKRGPALRAYCRYLIDISRRGNWQEIVVALNPCLMGYVYAVDKVKDKITAAE
GSIYSEWCDTCASSFCYQAVLEGERLMNHILETYPPDQLDSLVTIFARGCELETNFWT
AAMEYE"
gene <782045..>785671
/gene="AXL1"
/locus_tag="YPR122W"
/gene_synonym="FUS5; STE22"
/db_xref="GeneID:856240"
mRNA <782045..>785671
/gene="AXL1"
/locus_tag="YPR122W"
/gene_synonym="FUS5; STE22"
/product="Axl1p"
/transcript_id="NM_001184219.1"
/db_xref="GeneID:856240"
CDS 782045..785671
/gene="AXL1"
/locus_tag="YPR122W"
/gene_synonym="FUS5; STE22"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:12176366]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:12176366]"
/experiment="EXISTENCE:genetic interaction:GO:0007323
peptide pheromone maturation [PMID:7569998]"
/experiment="EXISTENCE:mutant phenotype:GO:0000755
cytogamy [PMID:9744878]"
/experiment="EXISTENCE:mutant phenotype:GO:0004222
metalloendopeptidase activity [PMID:7569998]"
/experiment="EXISTENCE:mutant phenotype:GO:0007120 axial
cellular bud site selection [PMID:7990931]"
/experiment="EXISTENCE:mutant phenotype:GO:0007323 peptide
pheromone maturation [PMID:7569998]"
/note="Haploid specific endoprotease of a-factor mating
pheromone; performs one of two N-terminal cleavages during
maturation of a-factor mating pheromone; required for
axial budding pattern of haploid cells"
/codon_start=1
/product="Axl1p"
/protein_id="NP_015447.1"
/db_xref="GeneID:856240"
/db_xref="SGD:S000006326"
/translation="MSLREVTNYEVSFYIPLSYSNRTHKVCKLPNGILALIISDPTDT
SSSCSLTVCTGSHNDPKDIAGLAHLCEHMILSAGSKKYPDPGLFHTLIAKNNGSQNAF
TTGEQTTFYFELPNTQNNGEFTFESILDVFASFFKEPLFNPLLISKEIYAIQSEHEGN
ISSTTKIFYHAARILANPDHPFSRFSTGNIHSLSSIPQLKKIKLKSSLNTYFENNFFG
ENITLCIRGPQSVNILTKLALSKFGDIKPKSAVKERSISIRTRSFRRSKSLKKRQDSS
KNDYSDLKTFKILNTTWEKKYKNTMCFQQFPECNSIFINSNKVPIMRLLFPVSDKNTR
FTKDDIKIYSHLWCELFGDESPGSLSYYLASKGWLTGCFAFTSEFAIGDIGLILELEL
TNSGWENIKRITTIVLNRLLPSFYVMNIDYLITFLKEQNLIDLVSFLYQSSEDLPMEE
CSKLSGILQDDLECLTPPNIFKGFKSLIEIDDPNIEKYENTKANIQWWTGQAIKFQNF
LKSFMNHDNMRLLLLGNIKSGNIFDKMKNKSDICTDFFYEFEYYTANVHLASDNKFHS
NSSYEFNFPTGNLFLPDCVSDPLKLQQLFLECSLKSKFATLRPQIYSEPTRTKPQLVS
ENQNYEMWILKEDPNFASDNKSVVSFEVLGLGIKPSPEATIHLEVLAQALFIITSSFL
YPALRIGYTYEIASSSKGNVTLRFTISGFPEGVFTIVKTFVDTLKLIATDPTFLSKDT
LRKARILVRNKYKNASSDNCVKLASVGLLIVLEKYIWTLEDRINALELTELESFEKFC
FLFWRNPKHLVLFMQGSLEYADAINRYLNNNFTQHLKISNEGSKPTIRLYPPPSTKDL
DQGTNAFISYNGHQDDPNNSIVYFIQTAQRDDIKNLTLTFLTEYLFSLTLVPDLRNKK
QIGYIVLGGLRVLTDTVGIHITVMSGSSGHNLETRINEYLSYLQLQVLNRFTEFDFRR
ILLEPFLNLLKQNSTKQFEGSAGPVDLLNEIVANVQNGDNYTLNNKQMRQHRKVRNKI
AEGRLNFQEDHEMIDISFLQKLTLKKYLAFFESKISIYSAQRSKLSIMITSPMAEKEI
ASRKMFLQLEAFLKINGFAIKNEDLKKIVEHSKGNPILLVKNLFTYFRRRNEVFKLGT
VVLQEILKIIGMNLKQRYGSILGFSSQDGEGQEIEKFWNNDTSPIVPLQELPEPNFFR
KAAF"
gene <786208..>787428
/gene="CTR1"
/locus_tag="YPR124W"
/db_xref="GeneID:856241"
mRNA <786208..>787428
/gene="CTR1"
/locus_tag="YPR124W"
/product="high-affinity Cu transporter CTR1"
/transcript_id="NM_001184221.1"
/db_xref="GeneID:856241"
CDS 786208..787428
/gene="CTR1"
/locus_tag="YPR124W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:22842922|PMID:7929270]"
/experiment="EXISTENCE:mutant phenotype:GO:0005375 copper
ion transmembrane transporter activity [PMID:7929270]"
/experiment="EXISTENCE:mutant phenotype:GO:0015677 copper
ion import [PMID:7929270]"
/note="High-affinity copper transporter of plasma
membrane; mediates nearly all copper uptake under low
copper conditions; transcriptionally induced at low copper
levels and degraded at high copper levels; protein
increases in abundance and relocalizes from nucleus to
plasma membrane upon DNA replication stress; human homolog
SLC31A1 can complement a yeast ctr1 ctr3 double deletion"
/codon_start=1
/product="high-affinity Cu transporter CTR1"
/protein_id="NP_015449.1"
/db_xref="GeneID:856241"
/db_xref="SGD:S000006328"
/translation="MEGMNMGSSMNMDAMSSASKTVASSMASMSMDAMSSASKTILSS
MSSMSMEAMSSASKTLASTMSSMASMSMGSSSMSGMSMSMSSTPTSSASAQTTSDSSM
SGMSGMSSSDNSSSSGMDMDMSMGMNYYLTPTYKNYPVLFHHLHANNSGKAFGIFLLF
VVAAFVYKLLLFVSWCLEVHWFKKWDKQNKYSTLPSANSKDEGKHYDTENNFEIQGLP
KLPNLLSDIFVPSLMDLFHDIIRAFLVFTSTMIIYMLMLATMSFVLTYVFAVITGLAL
SEVFFNRCKIAMLKRWDIQREIQKAKSCPGFGNCQCGRHPEPSPDPIAVADTTSGSDQ
STRLEKNNESKVAISENNQKKTPTQEEGCNCATDSGKNQANIERDILENSKLQEQSGN
MDQNLLPAEKFTHN"
gene <787961..>789325
/gene="YLH47"
/locus_tag="YPR125W"
/gene_synonym="MRS7"
/db_xref="GeneID:856243"
mRNA <787961..>789325
/gene="YLH47"
/locus_tag="YPR125W"
/gene_synonym="MRS7"
/product="Ylh47p"
/transcript_id="NM_001184222.1"
/db_xref="GeneID:856243"
CDS 787961..789325
/gene="YLH47"
/locus_tag="YPR125W"
/gene_synonym="MRS7"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:24769239|PMID:16823961|PMID:24390141|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:16476776]"
/experiment="EXISTENCE:genetic interaction:GO:0006813
potassium ion transport [PMID:20197279]"
/experiment="EXISTENCE:genetic interaction:GO:1902600
proton transmembrane transport [PMID:20197279]"
/experiment="EXISTENCE:mutant phenotype:GO:0032979 protein
insertion into mitochondrial inner membrane from matrix
[PMID:16476776]"
/note="Mitochondrial inner membrane protein; exposed to
the mitochondrial matrix; associates with mitochondrial
ribosomes; NOT required for respiratory growth; homolog of
human Letm1, a protein implicated in Wolf-Hirschhorn
syndrome"
/codon_start=1
/product="Ylh47p"
/protein_id="NP_015450.1"
/db_xref="GeneID:856243"
/db_xref="SGD:S000006329"
/translation="MLKYRSLPIKRAIHHPAPGITPISPRIMVSRLRVIPSFNLKFNR
WNSSVPESSKKELKTTDGNQESASKVSPVKEKEKVPFKVKMQKALRHYWDGSKLLGLE
IKISSKLLMKSAAGYPLTRRENLQLKRTTQDIVRLVPFAAFLIIPFAELLLPFALKLF
PNLLPSTYESSKKRENKLENLRNTRKLMSEIIKNNKSHFKPNNISEEQKALFNRFYTH
VRATGVPESRQQLIEVARLFTDDTVLDNVTRPYLIALAKYMNLQPFGTDVMLRYRIRY
KMLELKKDDLSIYYEDAEQLSLSELKTACASRGIRSVDVEPSVLYSNLRLWLNMRLKD
KIPSTLLIMATAYNYGNVQSKESLYDALCDVLIGIPDELYHEVKVNVVKEDEASAKQK
LKQLREQEEIMKEEEQQEENAIVSVKDELSLDDQDKNIDAAAPDVKPHDTKPIGEAAA
IKEK"
gene <790083..>791120
/locus_tag="YPR127W"
/db_xref="GeneID:856245"
mRNA <790083..>791120
/locus_tag="YPR127W"
/product="pyridoxine 4-dehydrogenase"
/transcript_id="NM_001184224.1"
/db_xref="GeneID:856245"
CDS 790083..791120
/locus_tag="YPR127W"
/EC_number="1.1.1.65"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="Putative pyridoxine 4-dehydrogenase; differentially
expressed during alcoholic fermentation; expression
activated by transcription factor YRM1/YOR172W; green
fluorescent protein (GFP)-fusion protein localizes to both
the cytoplasm and the nucleus"
/codon_start=1
/product="pyridoxine 4-dehydrogenase"
/protein_id="NP_015452.1"
/db_xref="GeneID:856245"
/db_xref="SGD:S000006331"
/translation="MSVADLKNNIHKLDTGYGLMSLTWRAEPIPQSQAFEAMHRVVEL
SRERGHKAFFNVGEFYGPDFINLSYVHDFFAKYPDLRKDVVISCKGGADNATLTPRGS
HDDVVQSVKNSVSAIGGYIDIFEVARIDTSLCTKGEVYPYESFEALAEMISEGVIGGI
SLSEVNEEQIRAIHKDWGKFLTCVEVELSLFSNDILHNGIAKTCAELGLSIICYSPLG
RGLLTGQLKSNADIPEGDFRKSLKRFSDESLKKNLTLVRFLQEEIVDKRPQNNSITLA
QLALGWVKHWNKVPEYSGAKFIPIPSGSSISKVNENFDEQKTKLTDQEFNAINKYLTT
FHTVGDRYEMA"
gene complement(<791218..>792204)
/gene="ANT1"
/locus_tag="YPR128C"
/db_xref="GeneID:856246"
mRNA complement(<791218..>792204)
/gene="ANT1"
/locus_tag="YPR128C"
/product="Ant1p"
/transcript_id="NM_001184225.1"
/db_xref="GeneID:856246"
CDS complement(791218..792204)
/gene="ANT1"
/locus_tag="YPR128C"
/experiment="EXISTENCE:direct assay:GO:0000295 adenine
nucleotide transmembrane transporter activity
[PMID:11566870]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005778 peroxisomal
membrane [PMID:11566870]"
/experiment="EXISTENCE:direct assay:GO:0015867 ATP
transport [PMID:11566870]"
/experiment="EXISTENCE:mutant phenotype:GO:0000295 adenine
nucleotide transmembrane transporter activity
[PMID:11390660]"
/experiment="EXISTENCE:mutant phenotype:GO:0006635 fatty
acid beta-oxidation [PMID:11390660|PMID:11566870]"
/experiment="EXISTENCE:mutant phenotype:GO:0007031
peroxisome organization [PMID:11390660]"
/note="Peroxisomal adenine nucleotide transporter;
involved in beta-oxidation of medium-chain fatty acid;
required for peroxisome proliferation"
/codon_start=1
/product="Ant1p"
/protein_id="NP_015453.1"
/db_xref="GeneID:856246"
/db_xref="SGD:S000006332"
/translation="MLTLESALTGAVASAMANIAVYPLDLSKTIIQSQVSPSSSEDSN
EGKVLPNRRYKNVVDCMINIFKEKGILGLYQGMTVTTVATFVQNFVYFFWYTFIRKSY
MKHKLLGLQSLKNRDGPITPSTIEELVLGVAAASISQLFTSPMAVVATRQQTVHSAES
AKFTNVIKDIYRENNGDITAFWKGLRTGLALTINPSITYASFQRLKEVFFHDHSNDAG
SLSAVQNFILGVLSKMISTLVTQPLIVAKAMLQSAGSKFTTFQEALLYLYKNEGLKSL
WKGVLPQLTKGVIVQGLLFAFRGELTKSLKRLIFLYSSFFLKHNGQRKLAST"
gene <792687..>793736
/gene="SCD6"
/locus_tag="YPR129W"
/gene_synonym="LSM13"
/db_xref="GeneID:856247"
mRNA <792687..>793736
/gene="SCD6"
/locus_tag="YPR129W"
/gene_synonym="LSM13"
/product="Scd6p"
/transcript_id="NM_001184226.1"
/db_xref="GeneID:856247"
CDS 792687..793736
/gene="SCD6"
/locus_tag="YPR129W"
/gene_synonym="LSM13"
/experiment="EXISTENCE:direct assay:GO:0000932 P-body
[PMID:22284680]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:22284680]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:22284680|PMID:26777405]"
/experiment="EXISTENCE:direct assay:GO:0031370 eukaryotic
initiation factor 4G binding [PMID:22284680]"
/experiment="EXISTENCE:direct assay:GO:0045947 negative
regulation of translational initiation [PMID:20832728]"
/experiment="EXISTENCE:mutant phenotype:GO:0033962 P-body
assembly [PMID:22284680]"
/experiment="EXISTENCE:mutant phenotype:GO:0034063 stress
granule assembly [PMID:22284680]"
/experiment="EXISTENCE:mutant phenotype:GO:0045947
negative regulation of translational initiation
[PMID:20832728]"
/note="Repressor of translation initiation; binds eIF4G
through its RGG domain and inhibits recruitment of the
preinitiation complex; also contains an Lsm domain; may
have a role in RNA processing; overproduction suppresses
null mutation in clathrin heavy chain gene CHC1; forms
cytoplasmic foci upon DNA replication stress"
/codon_start=1
/product="Scd6p"
/protein_id="NP_015454.1"
/db_xref="GeneID:856247"
/db_xref="SGD:S000006333"
/translation="MSQYIGKTISLISVTDNRYVGLLEDIDSEKGTVTLKEVRCFGTE
GRKNWGPEEIYPNPTVYNSVKFNGSEVKDLSILDANINDIQPVVPQMMPPASQFPPQQ
AQSPPQAQAQAHVQTNPQVPKPESNVPAAVAGYGVYTPTSTETATASMNDKSTPQDTN
VNSQSRERGKNGENEPKYQRNKNRSSNRPPQSNRNFKVDIPNEDFDFQSNNAKFTKGD
STDVEKEKELESAVHKQDESDEQFYNKKSSFFDTISTSTETNTNMRWQEEKMLNVDTF
GQASARPRFHSRGLGRGRGNYRGNRGNRGRGGQRGNYQNRNNYQNDSGAYQNQNDSYS
RPANQFSQPPSNVEF"
gene complement(<793911..>794498)
/gene="NAT3"
/locus_tag="YPR131C"
/gene_synonym="NAA20; RAD56"
/db_xref="GeneID:856249"
mRNA complement(<793911..>794498)
/gene="NAT3"
/locus_tag="YPR131C"
/gene_synonym="NAA20; RAD56"
/product="peptide alpha-N-acetyltransferase complex B
subunit NAT3"
/transcript_id="NM_001184228.1"
/db_xref="GeneID:856249"
CDS complement(793911..794498)
/gene="NAT3"
/locus_tag="YPR131C"
/gene_synonym="NAA20; RAD56"
/EC_number="2.3.1.254"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0031416 NatB
complex [PMID:12783868]"
/experiment="EXISTENCE:genetic interaction:GO:0000001
mitochondrion inheritance [PMID:12808144]"
/experiment="EXISTENCE:genetic interaction:GO:0032956
regulation of actin cytoskeleton organization
[PMID:12808144]"
/experiment="EXISTENCE:mutant phenotype:GO:0000001
mitochondrion inheritance [PMID:12808144]"
/experiment="EXISTENCE:mutant phenotype:GO:0004596
protein-N-terminal amino-acid acetyltransferase activity
[PMID:10545125]"
/experiment="EXISTENCE:mutant phenotype:GO:0017196
N-terminal peptidyl-methionine acetylation
[PMID:10545125]"
/experiment="EXISTENCE:mutant phenotype:GO:0032956
regulation of actin cytoskeleton organization
[PMID:12808144]"
/experiment="EXISTENCE:mutant phenotype:GO:0036503 ERAD
pathway [PMID:23363603]"
/note="Catalytic subunit of the NatB N-terminal
acetyltransferase; NatB catalyzes acetylation of the
amino-terminal methionine residues of all proteins
beginning with Met-Asp or Met-Glu and of some proteins
beginning with Met-Asn or Met-Met"
/codon_start=1
/product="peptide alpha-N-acetyltransferase complex B
subunit NAT3"
/protein_id="NP_015456.2"
/db_xref="GeneID:856249"
/db_xref="SGD:S000006335"
/translation="MTTIQPFEPVDLFKTNNVNLDILTENFPLEFYFEYMIIWPDLFF
KSSEMTVDPTFKHNISGYMMAKTEGKTTEWHTHITAVTVAPRFRRISLASKLCNTLET
MTDVMPHEVNFIDLFVKCNNQLAIKLYEKLGYSVYRRVVGYYNSAEDGYPDTLKKVDD
NKDAFDMRKAMARDRNRSVRPDGRSHKCYPHDVRF"
gene <794965..>795767
/gene="RPS23B"
/locus_tag="YPR132W"
/db_xref="GeneID:856250"
mRNA join(<794965..795029,795395..>795767)
/gene="RPS23B"
/locus_tag="YPR132W"
/product="40S ribosomal protein uS12 RPS23B"
/transcript_id="NM_001184229.1"
/db_xref="GeneID:856250"
CDS join(794965..795029,795395..795767)
/gene="RPS23B"
/locus_tag="YPR132W"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:6814480]"
/experiment="EXISTENCE:direct assay:GO:0022627 cytosolic
small ribosomal subunit [PMID:6814480]"
/experiment="EXISTENCE:genetic interaction:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16246728]"
/experiment="EXISTENCE:genetic interaction:GO:0006450
regulation of translational fidelity
[PMID:8415737|PMID:8950190]"
/experiment="EXISTENCE:mutant phenotype:GO:0006450
regulation of translational fidelity
[PMID:8415737|PMID:8950190]"
/note="Ribosomal protein 28 (rp28) of the small (40S)
ribosomal subunit; required for translational accuracy;
homologous to mammalian ribosomal protein S23 and
bacterial S12; RPS23B has a paralog, RPS23A, that arose
from the whole genome duplication; deletion of both RPS23A
and RPS23B is lethal"
/codon_start=1
/product="40S ribosomal protein uS12 RPS23B"
/protein_id="NP_015457.1"
/db_xref="GeneID:856250"
/db_xref="SGD:S000006336"
/translation="MGKGKPRGLNSARKLRVHRRNNRWAENNYKKRLLGTAFKSSPFG
GSSHAKGIVLEKLGIESKQPNSAIRKCVRVQLIKNGKKVTAFVPNDGCLNFVDENDEV
LLAGFGRKGKAKGDIPGVRFKVVKVSGVSLLALWKEKKEKPRS"
gene complement(<795978..>797210)
/gene="SPN1"
/locus_tag="YPR133C"
/gene_synonym="IWS1"
/db_xref="GeneID:856251"
mRNA complement(<795978..>797210)
/gene="SPN1"
/locus_tag="YPR133C"
/gene_synonym="IWS1"
/product="transcription factor SPN1"
/transcript_id="NM_001184230.1"
/db_xref="GeneID:856251"
CDS complement(795978..797210)
/gene="SPN1"
/locus_tag="YPR133C"
/gene_synonym="IWS1"
/experiment="EXISTENCE:curator inference:GO:0005634
nucleus [PMID:12556496|PMID:18086892|PMID:12524336]"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:29300974]"
/experiment="EXISTENCE:genetic interaction:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:12556496|PMID:12524336]"
/experiment="EXISTENCE:genetic interaction:GO:0034243
regulation of transcription elongation by RNA polymerase
II [PMID:18086892]"
/experiment="EXISTENCE:mutant phenotype:GO:0005665 RNA
polymerase II, core complex [PMID:32941642]"
/experiment="EXISTENCE:mutant phenotype:GO:0006334
nucleosome assembly [PMID:29300974]"
/experiment="EXISTENCE:mutant phenotype:GO:0016973
poly(A)+ mRNA export from nucleus [PMID:19034519]"
/experiment="EXISTENCE:physical interaction:GO:0000993 RNA
polymerase II complex binding
[PMID:12556496|PMID:18086892]"
/experiment="EXISTENCE:physical interaction:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:12556496]"
/experiment="EXISTENCE:physical interaction:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:12556496]"
/note="Protein involved in RNA polymerase II
transcription; also required for histone modifications and
splicing; constitutively recruited to the CYC1 promoter
and required for recruitment of chromatin remodeling
factors for the expression of CYC1 gene; interacts
genetically or physically with RNAP II, TBP, TFIIS, and
chromatin remodelling factors; central domain is highly
conserved throughout eukaryotes; mutations confer an Spt-
phenotype"
/codon_start=1
/product="transcription factor SPN1"
/protein_id="NP_015458.1"
/db_xref="GeneID:856251"
/db_xref="SGD:S000006337"
/translation="MSTADQEQPKVVEATPEDGTASSQKSTINAENENTKQNQSMEPQ
ETSKGTSNDTKDPDNGEKNEEAAIDENSNVEAAERKRKHISTDFSDDDLEKEEHNDQS
LQPTVENRASKDRDSSATPSSRQELEEKLDRILKKPKVRRTRRDEDDLEQYLDEKILR
LKDEMNIAAQLDIDTLNKRIETGDTSLIAMQKVKLLPKVVSVLSKANLADTILDNNLL
QSVRIWLEPLPDGSLPSFEIQKSLFAALNDLPVKTEHLKESGLGRVVIFYTKSKRVEA
QLARLAEKLIAEWTRPIIGASDNYRDKRIMQLEFDSEKLRKKSVMDSAKNRKKKSKSG
EDPTSRGSSVQTLYEQAAARRNRAAAPAQTTTDYKYAPVSNLSAVPTNARAVGVGSTL
NNSEMYKRLTSRLNKKHK"
gene <797557..>797709
/gene="TOM5"
/locus_tag="YPR133W-A"
/gene_synonym="MOM8A"
/db_xref="GeneID:856252"
mRNA <797557..>797709
/gene="TOM5"
/locus_tag="YPR133W-A"
/gene_synonym="MOM8A"
/product="Tom5p"
/transcript_id="NM_001184307.1"
/db_xref="GeneID:856252"
CDS 797557..797709
/gene="TOM5"
/locus_tag="YPR133W-A"
/gene_synonym="MOM8A"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:9217162|PMID:16407407]"
/experiment="EXISTENCE:direct assay:GO:0005742
mitochondrial outer membrane translocase complex
[PMID:9217162]"
/experiment="EXISTENCE:direct assay:GO:0070096
mitochondrial outer membrane translocase complex assembly
[PMID:20668160]"
/experiment="EXISTENCE:mutant phenotype:GO:0006626 protein
targeting to mitochondrion [PMID:9217162]"
/experiment="EXISTENCE:mutant phenotype:GO:0008320 protein
transmembrane transporter activity [PMID:9217162]"
/note="Component of the TOM (translocase of outer
membrane) complex; responsible for recognition and initial
import of all mitochondrially directed proteins; involved
in transfer of precursors from the Tom70p and Tom20p
receptors to the Tom40p pore"
/codon_start=1
/product="Tom5p"
/protein_id="NP_015459.1"
/db_xref="GeneID:856252"
/db_xref="SGD:S000006433"
/translation="MFGLPQQEVSEEEKRAHQEQTEKTLKQAAYVAAFLWVSPMIWHL
VKKQWK"
gene <798051..>798857
/gene="MSS18"
/locus_tag="YPR134W"
/db_xref="GeneID:856253"
mRNA <798051..>798857
/gene="MSS18"
/locus_tag="YPR134W"
/product="Mss18p"
/transcript_id="NM_001184231.1"
/db_xref="GeneID:856253"
CDS 798051..798857
/gene="MSS18"
/locus_tag="YPR134W"
/experiment="EXISTENCE:direct assay:GO:0000372 Group I
intron splicing [PMID:2842150]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:mutant phenotype:GO:0000372 Group I
intron splicing [PMID:2842150]"
/experiment="EXISTENCE:mutant phenotype:GO:0090615
mitochondrial mRNA processing [PMID:21257754]"
/note="Nuclear encoded protein needed for splicing of
mitochondrial intron; required for efficient splicing of
mitochondrial COX1 aI5beta intron; mss18 mutations block
cleavage of 5' exon - intron junction; phenotype of
intronless strain suggests additional functions"
/codon_start=1
/product="Mss18p"
/protein_id="NP_015460.1"
/db_xref="GeneID:856253"
/db_xref="SGD:S000006338"
/translation="MGLPEVNFLRKNCILVELKLFYQTVYPPKELYWNNRITAELSTF
SNIKYARPTFAVNNGTFQRTRPKLDLVLASSDIRKLATVLFNLKALIMNTKGEEPTLT
TMTSVQTNEEKNDNLEQKYSSLLDRWNGKVEVHDSPFLQLQRDSNLLFAERPVRYVST
TEGEGVDISSEEFFRLEEEQCRRNYDVLVDEHSTPSVGMKDGQYGPNIIHFEPSLYHT
YSSLPMSMKFWLNGLEDDETTMMNIDEKSAENLDILLHGFKGFSNKRVKG"
gene <799234..>802017
/gene="CTF4"
/locus_tag="YPR135W"
/gene_synonym="CHL15; POB1"
/db_xref="GeneID:856254"
mRNA <799234..>802017
/gene="CTF4"
/locus_tag="YPR135W"
/gene_synonym="CHL15; POB1"
/product="chromatin-binding protein CTF4"
/transcript_id="NM_001184232.1"
/db_xref="GeneID:856254"
CDS 799234..802017
/gene="CTF4"
/locus_tag="YPR135W"
/gene_synonym="CHL15; POB1"
/experiment="EXISTENCE:direct assay:GO:0000228 nuclear
chromosome [PMID:11287619]"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:20381454]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0043596 nuclear
replication fork [PMID:16962805]"
/experiment="EXISTENCE:genetic interaction:GO:0007064
mitotic sister chromatid cohesion
[PMID:17483413|PMID:14742714]"
/experiment="EXISTENCE:mutant phenotype:GO:0000727
double-strand break repair via break-induced replication
[PMID:20516198]"
/experiment="EXISTENCE:mutant phenotype:GO:0006261
DNA-templated DNA replication [PMID:1448101]"
/experiment="EXISTENCE:mutant phenotype:GO:0006270 DNA
replication initiation [PMID:20980819]"
/experiment="EXISTENCE:mutant phenotype:GO:0006281 DNA
repair [PMID:1341195|PMID:1448101]"
/experiment="EXISTENCE:mutant phenotype:GO:0007064 mitotic
sister chromatid cohesion [PMID:11287619]"
/experiment="EXISTENCE:mutant phenotype:GO:0034085
establishment of sister chromatid cohesion
[PMID:23334284]"
/note="Chromatin-associated protein; required for sister
chromatid cohesion; interacts with DNA polymerase alpha
(Pol1p); recruits Mms22p to replication forks during S
phase, and may link DNA synthesis to sister chromatid
cohesion"
/codon_start=1
/product="chromatin-binding protein CTF4"
/protein_id="NP_015461.1"
/db_xref="GeneID:856254"
/db_xref="SGD:S000006339"
/translation="MVSVIDKLVFDFGGKTLVSLAPDNNTLCVANKNGLTKILKTNNP
EEEPETLDSSKLVSSIKCYSNSHFLMTTMQGDALRYNIDSSQEELLARFALPLRDCCV
IHSGKMAVFGGDDLELILLELDDETHKKHAIKIDEQVSQISYNSQMNILAVSMINGKV
QIFSLTSTIPNKVHELNDYIVANSYDDTHRDKILSNMMDDIDKDNDNDLSETADPDEN
NVADPEFCAANRICTRVAWHPKGLHFALPCADDTVKIFSIKGYSLQKTLSTNLSSTKA
HFIDLQFDPLRGTYIAAVDLNNKLTVWNWETSEIHYTREFKRKITNIAWKIQADSKTL
DLVLGTWSGSIAIVQNLAESVVSNIPDQSVAESSTKHGLFVDSESDLENLEGNDDINK
SDKLFSDITQEANAEDVFTQTHDGPSGLSEKRKYNFEDEEDFIDDDDGAGYISGKKPH
NEHSYSRVHKTHSFPISLANTGKFRYMPFSPAGTPFGFTDRRYLTMNEVGYVSTVKNS
EQYSITVSFFDVGRFREYHFEDLFGYDLCFLNEKGTLFGQSKTGQIQYRPHDSIHSNW
TKIIPLQAGERITSVAATPVRVIVGTSLGYFRSFNQFGVPFAVEKTSPIVALTAQNYR
VFSVHYSQFHGLSYSLSELGTSSKRYYKRECPLPMSLPNINSDMKKDANLDYYNFNPM
GIKSLFFSSYGDPCIFGSDNTLLLLSKWRSPEESKWLPILDSNMEIWKMSGGKETTDI
HVWPLALAYDTLNCILVKGKHIWPEFPLPLPSEMEIRMPVFVKSKLLEENKAILNKKN
EIGADTEAEEGEEDKEIQIPVSMAAEEEYLRSKVLSELLTDTLENDGEMYGNENEVLA
ALNGAYDKALLRLFASACSDQNVEKALSLAHELKQDRALTAAVKISERAELPSLVKKI
NNIREARYEQQLK"
gene <802359..>804080
/gene="RRP9"
/locus_tag="YPR137W"
/db_xref="GeneID:856255"
mRNA <802359..>804080
/gene="RRP9"
/locus_tag="YPR137W"
/product="ribosomal RNA-processing protein RRP9"
/transcript_id="NM_001184234.1"
/db_xref="GeneID:856255"
CDS 802359..804080
/gene="RRP9"
/locus_tag="YPR137W"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:12150911]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/experiment="EXISTENCE:direct assay:GO:0031428 box C/D
methylation guide snoRNP complex [PMID:11081632]"
/experiment="EXISTENCE:direct assay:GO:0032040
small-subunit processome [PMID:12068309]"
/experiment="EXISTENCE:direct assay:GO:0034511 U3 snoRNA
binding [PMID:23509373]"
/experiment="EXISTENCE:mutant phenotype:GO:0000447
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from
5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11105764]"
/experiment="EXISTENCE:mutant phenotype:GO:0000472
endonucleolytic cleavage to generate mature 5'-end of
SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:11105764]"
/experiment="EXISTENCE:mutant phenotype:GO:0000480
endonucleolytic cleavage in 5'-ETS of tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:11105764]"
/note="Protein involved in pre-rRNA processing; associated
with U3 snRNP; component of small ribosomal subunit (SSU)
processome; ortholog of the human U3-55k protein"
/codon_start=1
/product="ribosomal RNA-processing protein RRP9"
/protein_id="NP_015463.1"
/db_xref="GeneID:856255"
/db_xref="SGD:S000006341"
/translation="MSDVTQQKKRKRSKGEVNPSKPTVDEEITDPSSNEDEQLEVSDE
EDALESEEEFEGENPADKRRRLAKQYLENLKSEANDILTDNRNAEEKDLNNLKERTID
EYNNFDAGDLDKDIIASRLKEDVAEQQGRVFRYFGDKLLISEAKQSFTRVGENNLTCI
SCFQPVLNKYTFEESSNGDKNKGRLFAYTVSKDLQLTKYDITDFSKRPKKLKYAKGGA
KYIPTSKHEYENTTEGHYDEILTVAASPDGKYVVTGGRDRKLIVWSTESLSPVKVIPT
KDRRGEVLSLAFRKNSDQLYASCADFKIRTYSINQFSQLEILYGHHDIVEDISALAME
RCVTVGARDRTAMLWKIPDETRLTFRGGDEPQKLLRRWMKENAKEGEDGEVKYPDESE
APLFFCEGSIDVVSMVDDFHFITGSDNGNICLWSLAKKKPIFTERIAHGILPEPSFND
ISGETDEELRKRQLQGKKLLQPFWITSLYAIPYSNVFISGSWSGSLKVWKISDNLRSF
ELLGELSGAKGVVTKIQVVESGKHGKEKFRILASIAKEHRLGRWIANVSGARNGIYSA
VIDQTGF"
repeat_region 804511..804638
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007213"
mobile_element complement(804645..810562)
/note="YPRCTy1-2; Ty1 element, LTR retrotransposon of the
Copia (Pseudoviridae) group; contains co-transcribed genes
TYA Gag and TYB Pol, encoding proteins involved in
structure and function of virus-like particles, flanked by
two direct repeats"
/mobile_element_type="retrotransposon:YPRCTy1-2"
/db_xref="SGD:S000007208"
repeat_region complement(804645..804978)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007202"
gene complement(<805001..>810269)
/locus_tag="YPR137C-B"
/db_xref="GeneID:856257"
mRNA complement(<805001..>810269)
/locus_tag="YPR137C-B"
/product="gag-pol fusion protein"
/transcript_id="NM_001184394.2"
/db_xref="GeneID:856257"
CDS complement(join(805001..808963,808965..810269))
/locus_tag="YPR137C-B"
/EC_number="2.7.7.7"
/EC_number="2.7.7.49"
/EC_number="3.1.26.4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9448009]"
/ribosomal_slippage
/note="Retrotransposon TYA Gag and TYB Pol genes;
transcribed/translated as one unit; polyprotein is
processed to make a nucleocapsid-like protein (Gag),
reverse transcriptase (RT), protease (PR), and integrase
(IN); similar to retroviral genes"
/codon_start=1
/product="gag-pol fusion protein"
/protein_id="NP_058191.1"
/db_xref="GeneID:856257"
/db_xref="SGD:S000007360"
/translation="MESQQLSQHSPISHGSACASVTSKEVHTNQDPLDVSASKTEECE
KASTKANSQQTTTPASSAVPENPHHASPQTAQSHSPQNGPYPQQCMMTQNQANPSGWS
FYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVRQITDDELT
FLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQ
YNGSTPADAFETKVTNIIDRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNM
TVAELFLDIHAIYEEQQGSRNSKPNYRRNPSDEKNDSRSYTNTTKPKVIARNPQKTNN
SKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLGQKLTESTVNHTNHS
DDELPGHLLLDSGASRTLIRSAHHIHSASSNPDINVVDAQKRNIPINAIGDLQFHFQD
NTKTSIKVLHTPNIAYDLLSLNELAAVDITACFTKNVLERSDGTVLAPIVKYGDFYWV
SKKYLLPSNISVPTINNVHTSESTRKYPYPFIHRMLAHANAQTIRYSLKNNTITYFNE
SDVDWSSAIDYQCPDCLIGKSTKHRHIKGSRLKYQNSYEPFQYLHTDIFGPVHNLPKS
APSYFISFTDETTKFRWVYPLHDRREDSILDVFTTILAFIKNQFQASVLVIQMDRGSE
YTNRTLHKFLEKNGITPCYTTTADSRAHGVAERLNRTLLDDCRTQLQCSGLPNHLWFS
AIEFSTIVRNSLASPKSKKSARQHAGLAGLDISTLLPFGQPVIVNDHNPNSKIHPRGI
PGYALHPSRNSYGYIIYLPSLKKTVDTTNYVILQGKESRLDQFNYDALTFDEDLNRLT
ASYHSFIASNEIQESNDLNIESDHDFQSDIELHPEQPRNVLSKAVSPTDSTPPSTHTE
DSKRVSKTNIRAPREVDPNISESNILPSKKRSSTPQISNIESTGSGGMHKLNVPLLAP
MSQSNTHESSHASKSKDFRHSDSYSENETNHTNVPISSTGGTNNKTVPQISDQETEKR
IIHRSPSIDASPPENNSSHNIVPIKTPTTVSEQNTEESIIADLPLPDLPPESPTEFPD
PFKELPPINSHQTNSSLGGIGDSNAYTTINSKKRSLEDNETEIKVSRDTWNTKNMRSL
EPPRSKKRIHLIAAVKAVKSIKPIRTTLRYDEAITYNKDIKEKEKYIEAYHKEVNQLL
KMNTWDTDKYYDRKEIDPKRVINSMFIFNKKRDGTHKARFVARGDIQHPDTYDTGMQS
NTVHHYALMTSLSLALDNNYYITQLDISSAYLYADIKEELYIRPPPHLGMNDKLIRLK
KSHYGLKQSGANWYETIKSYLIKQCGMEEVRGWSCVFKNSQVTICLFVDDMILFSKDL
NANKKIITTLKKQYDTKIINLGESDNEIQYDILGLEIKYQRGKYMKLGMENSLTEKIP
KLNVPLNPKGRKLSAPGQPGLYIDQDELEIDEDEYKEKVHEMQKLIGLASYVGYKFRF
DLLYYINTLAQHILFPSRQVLDMTYELIQFMWDTRDKQLIWHKNKPTEPDNKLVAISD
ASYGNQPYYKSQIGNIFLLNGKVIGGKSTKASLTCTSTTEAEIHAVSEAIPLLNNLSH
LVQELNKKPIIKGLLTDSRSTISIIKSTNEEKFRNRFFGTKAMRLRDEVSGNNLYVYY
IETKKNIADVMTKPLPIKTFKLLTNKWIH"
gene complement(<808947..>810269)
/locus_tag="YPR137C-A"
/db_xref="GeneID:856258"
mRNA complement(<808947..>810269)
/locus_tag="YPR137C-A"
/product="gag protein"
/transcript_id="NM_001184393.1"
/db_xref="GeneID:856258"
CDS complement(808947..810269)
/locus_tag="YPR137C-A"
/note="Retrotransposon TYA Gag gene co-transcribed with
TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is
a nucleocapsid protein that is the structural constituent
of virus-like particles (VLPs); similar to retroviral Gag"
/codon_start=1
/product="gag protein"
/protein_id="NP_058192.1"
/db_xref="GeneID:856258"
/db_xref="SGD:S000007359"
/translation="MESQQLSQHSPISHGSACASVTSKEVHTNQDPLDVSASKTEECE
KASTKANSQQTTTPASSAVPENPHHASPQTAQSHSPQNGPYPQQCMMTQNQANPSGWS
FYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVRQITDDELT
FLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQ
YNGSTPADAFETKVTNIIDRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNM
TVAELFLDIHAIYEEQQGSRNSKPNYRRNPSDEKNDSRSYTNTTKPKVIARNPQKTNN
SKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLRPETY"
repeat_region complement(810229..810562)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007203"
gene 810676..810749
/locus_tag="YNCP0019W"
/db_xref="GeneID:856259"
tRNA 810676..810749
/locus_tag="YNCP0019W"
/product="tRNA-Asn"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Asparagine tRNA (tRNA-Asn), predicted by
tRNAscan-SE analysis"
/db_xref="GeneID:856259"
/db_xref="SGD:S000006677"
gene complement(<810984..>812453)
/gene="MEP3"
/locus_tag="YPR138C"
/db_xref="GeneID:856260"
mRNA complement(<810984..>812453)
/gene="MEP3"
/locus_tag="YPR138C"
/product="ammonium permease MEP3"
/transcript_id="NM_001184235.1"
/db_xref="GeneID:856260"
CDS complement(810984..812453)
/gene="MEP3"
/locus_tag="YPR138C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0008519 ammonium
channel activity [PMID:9234685]"
/experiment="EXISTENCE:mutant phenotype:GO:0008519
ammonium channel activity [PMID:9234685]"
/experiment="EXISTENCE:mutant phenotype:GO:0019740
nitrogen utilization [PMID:9234685]"
/experiment="EXISTENCE:mutant phenotype:GO:0072488
ammonium transmembrane transport [PMID:9234685]"
/note="Ammonium permease of high capacity and low
affinity; belongs to Mep-Amt-Rh family of well-conserved
ammonium (NH4+) transporters that includes the human Rh
factors; expression is under the nitrogen catabolite
repression regulation ammonia permease activity regulated
by TORC1 effectors, Npr1p and Par32p; MEP3 has a paralog,
MEP1, that arose from the whole genome duplication"
/codon_start=1
/product="ammonium permease MEP3"
/protein_id="NP_015464.1"
/db_xref="GeneID:856260"
/db_xref="SGD:S000006342"
/translation="MARGDGHLWTETYDSSTVAFMILGAALVFFMVPGLGFLYSGLAR
RKSALALIWVVIMATLVGILQWYFWGYSLAFSKTATNNKFIGNLDSFGFRNVYGKISD
DSTYPELIYAIFQMMFMCVALSIIAGATAERGKLFPHMVFLFVFATLVYCPITYWIWA
PGGWAYQWGVLDWAGGGNIEILSAVAGFVYSYFLGRRKENLLINFRPHNVSMVTLGTS
ILWFGWLLFNAASSLSPNMRSVYAFMNTCLSATTGGMTWCLLDYRSEKKWSTVGLCSG
IICGLVAATPSSGCITLYGSLIQGIIAGVVCNFATKIKYYLKVDDSLDLLAEHGIAGV
VGLIFNALFAADWVIGMDGTTKHKGGWLTHNWKQMYIQIAYIGASAGYCAVVTAIICF
VLGKIPGVHLRVTEEAEALGLDEDQIGEFAYDYVEVRRDYYQWGVDTDALHTTCNGAN
SASETNPTEDSQNSSLSSATVSGQNEKSNNPKLHHAKEA"
gene complement(<813156..>814058)
/gene="LOA1"
/locus_tag="YPR139C"
/gene_synonym="VPS66"
/db_xref="GeneID:856261"
mRNA complement(<813156..>814058)
/gene="LOA1"
/locus_tag="YPR139C"
/gene_synonym="VPS66"
/product="lysophosphatidic acid acyltransferase LOA1"
/transcript_id="NM_001184236.1"
/db_xref="GeneID:856261"
CDS complement(813156..814058)
/gene="LOA1"
/locus_tag="YPR139C"
/gene_synonym="VPS66"
/EC_number="2.3.1.51"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:22090344|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005811 lipid
droplet [PMID:21820081|PMID:22090344]"
/experiment="EXISTENCE:direct assay:GO:0042171
lysophosphatidic acid acyltransferase activity
[PMID:22090344]"
/experiment="EXISTENCE:mutant phenotype:GO:0006623 protein
targeting to vacuole [PMID:12134085]"
/experiment="EXISTENCE:mutant phenotype:GO:0034389 lipid
droplet organization [PMID:22090344]"
/experiment="EXISTENCE:mutant phenotype:GO:0035356
intracellular triglyceride homeostasis [PMID:22090344]"
/note="Lysophosphatidic acid acyltransferase; involved in
triacelglyceride homeostasis and lipid droplet formation;
localized to lipid droplets and the ER; specificity for
oleoyl-CoA"
/codon_start=1
/product="lysophosphatidic acid acyltransferase LOA1"
/protein_id="NP_015465.1"
/db_xref="GeneID:856261"
/db_xref="SGD:S000006343"
/translation="MEKYTNWRDNGTGIAPFLPNTIRKPSKVMTACLLGILGVKTIIM
LPLIMLYLLTGQNNLLGLILKFTFSWKEEITVQGIKKRDVRKSKHYPQKGKLYICNCT
SPLDAFSVVLLAQGPVTLLVPSNDIVYKVSIREFINFILAGGLDIKLYGHEVAELSQL
GNTVNFMFAEGTSCNGKSVLPFSITGKKLKEFIDPSITTMNPAMAKTKKFELQTIQIK
TNKTAITTLPISNMEYLSRFLNKGINVKCKINEPQVLSDNLEELRVALNGGDKYKLVS
RKLDVESKRNFVKEYISDQRKKRK"
gene <814391..>815536
/gene="TAZ1"
/locus_tag="YPR140W"
/db_xref="GeneID:856262"
mRNA <814391..>815536
/gene="TAZ1"
/locus_tag="YPR140W"
/product="lysophosphatidylcholine acyltransferase"
/transcript_id="NM_001184237.1"
/db_xref="GeneID:856262"
CDS 814391..815536
/gene="TAZ1"
/locus_tag="YPR140W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:14562095|PMID:15588229|PMID:14576278|PMID:24769239|P
MID:26928762|PMID:15304507|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane
[PMID:16880272|PMID:16135531]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:16880272]"
/experiment="EXISTENCE:direct assay:GO:0035965 cardiolipin
acyl-chain remodeling [PMID:27268057]"
/experiment="EXISTENCE:direct assay:GO:0047184
1-acylglycerophosphocholine O-acyltransferase activity
[PMID:27268057]"
/experiment="EXISTENCE:mutant phenotype:GO:0005739
mitochondrion [PMID:15304507]"
/experiment="EXISTENCE:mutant phenotype:GO:0007007 inner
mitochondrial membrane organization [PMID:14651618]"
/experiment="EXISTENCE:mutant phenotype:GO:0008654
phospholipid biosynthetic process
[PMID:14651618|PMID:15588229]"
/experiment="EXISTENCE:mutant phenotype:GO:0032048
cardiolipin metabolic process [PMID:14651618]"
/experiment="EXISTENCE:mutant phenotype:GO:0035965
cardiolipin acyl-chain remodeling [PMID:14651618]"
/experiment="EXISTENCE:mutant phenotype:GO:0042773 ATP
synthesis coupled electron transport [PMID:15304507]"
/experiment="EXISTENCE:mutant phenotype:GO:0047184
1-acylglycerophosphocholine O-acyltransferase activity
[PMID:15588229]"
/experiment="EXISTENCE:mutant phenotype:GO:0097250
mitochondrial respirasome assembly [PMID:16135531]"
/note="Lyso-phosphatidylcholine acyltransferase; required
for normal phospholipid content of mitochondrial
membranes; major determinant of the final acyl chain
composition of the mitochondrial-specific phospholipid
cardiolipin; mutations in human ortholog tafazzin (TAZ)
cause Barth syndrome, a rare X-linked disease
characterized by skeletal and cardiomyopathy and bouts of
cyclic neutropenia; a specific splice variant of human TAZ
can complement yeast null mutant"
/codon_start=1
/product="lysophosphatidylcholine acyltransferase"
/protein_id="NP_015466.1"
/db_xref="GeneID:856262"
/db_xref="SGD:S000006344"
/translation="MSFRDVLERGDEFLEAYPRRSPLWRFLSYSTSLLTFGVSKLLLF
TCYNVKLNGFEKLETALERSKRENRGLMTVMNHMSMVDDPLVWATLPYKLFTSLDNIR
WSLGAHNICFQNKFLANFFSLGQVLSTERFGVGPFQGSIDASIRLLSPDDTLDLEWTP
HSEVSSSLKKAYSPPIIRSKPSWVHVYPEGFVLQLYPPFENSMRYFKWGITRMILEAT
KPPIVVPIFATGFEKIASEAVTDSMFRQILPRNFGSEINVTIGDPLNDDLIDRYRKEW
THLVEKYYDPKNPNDLSDELKYGKEAQDLRSRLAAELRAHVAEIRNEVRKLPREDPRF
KSPSWWKRFNTTEGKSDPDVKVIGENWAIRRMQKFLPPEGKPKGKDD"
gene complement(<815734..>817923)
/gene="KAR3"
/locus_tag="YPR141C"
/gene_synonym="OSR11"
/db_xref="GeneID:856263"
mRNA complement(<815734..>817923)
/gene="KAR3"
/locus_tag="YPR141C"
/gene_synonym="OSR11"
/product="Kar3p"
/transcript_id="NM_001184238.1"
/db_xref="GeneID:856263"
CDS complement(815734..817923)
/gene="KAR3"
/locus_tag="YPR141C"
/gene_synonym="OSR11"
/EC_number="5.6.1.4"
/experiment="EXISTENCE:direct assay:GO:0000922 spindle
pole [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:26928762|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005816 spindle
pole body [PMID:8106549|PMID:11729143|PMID:2138512]"
/experiment="EXISTENCE:direct assay:GO:0005874 microtubule
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005881 cytoplasmic
microtubule [PMID:2138512]"
/experiment="EXISTENCE:direct assay:GO:0008017 microtubule
binding [PMID:22734002]"
/experiment="EXISTENCE:direct assay:GO:0008569
minus-end-directed microtubule motor activity
[PMID:8041770]"
/experiment="EXISTENCE:direct assay:GO:0044732 mitotic
spindle pole body [PMID:16533946]"
/experiment="EXISTENCE:direct assay:GO:0051321 meiotic
cell cycle [PMID:11729143]"
/experiment="EXISTENCE:genetic interaction:GO:0007064
mitotic sister chromatid cohesion [PMID:14742714]"
/experiment="EXISTENCE:mutant phenotype:GO:0000278 mitotic
cell cycle [PMID:9201713]"
/experiment="EXISTENCE:mutant phenotype:GO:0000742
karyogamy involved in conjugation with cellular fusion
[PMID:2138512]"
/experiment="EXISTENCE:mutant phenotype:GO:0000743 nuclear
migration involved in conjugation with cellular fusion
[PMID:2138512|PMID:19386762|PMID:16380440]"
/experiment="EXISTENCE:mutant phenotype:GO:0007064 mitotic
sister chromatid cohesion [PMID:14742714]"
/experiment="EXISTENCE:mutant phenotype:GO:0036503 ERAD
pathway [PMID:39841550]"
/experiment="EXISTENCE:mutant phenotype:GO:0051321 meiotic
cell cycle [PMID:11729143]"
/experiment="EXISTENCE:mutant phenotype:GO:0060236
regulation of mitotic spindle organization
[PMID:25313961]"
/note="Minus-end-directed microtubule motor; functions in
mitosis and meiosis; localizes to the spindle pole body
and localization is dependent on functional Cik1p;
required for nuclear fusion during mating; required for
ER-associated degradation (ERAD); potential Cdc28p
substrate; kinesin 14 family member with a C-terminal
motor domain"
/codon_start=1
/product="Kar3p"
/protein_id="NP_015467.1"
/db_xref="GeneID:856263"
/db_xref="SGD:S000006345"
/translation="MESLPRTPTKGRSTQHLSTPSPKNDILAMNGHKRRNTTTPPPKH
TLLKPQRTDIHRHSLASQSRISMSPNRELLKNYKGTANLIYGNQKSNSGVTSFYKENV
NELNRTQAILFEKKATLDLLKDELTETKEKINAVNLKFETLREEKIKIEQQLNLKNNE
LISIKEEFLSKKQFMNEGHEIHLKQLAASNKKELKQMENEYKTKIEKLKFMKIKQFEN
ERASLLDKIEEVRNKITMNPSTLQEMLNDVEQKHMLEKEEWLTEYQSQWKKDIELNNK
HMQEIESIKKEIENTLKPELAEKKKLLTEKRNAYEAIKVKVKEKEEETTRLRDEVALK
QKTNLETLEKIKELEEYIKDTELGMKELNEILIKEETVRRTLHNELQELRGNIRVYCR
IRPALKNLENSDTSLINVNEFDDNSGVQSMEVTKIQNTAQVHEFKFDKIFDQQDTNVD
VFKEVGQLVQSSLDGYNVCIFAYGQTGSGKTFTMLNPGDGIIPSTISHIFNWINKLKT
KGWDYKVNCEFIEIYNENIVDLLRSDNNNKEDTSIGLKHEIRHDQETKTTTITNVTSC
KLESEEMVEIILKKANKLRSTASTASNEHSSRSHSIFIIHLSGSNAKTGAHSYGTLNL
VDLAGSERINVSQVVGDRLRETQNINKSLSCLGDVIHALGQPDSTKRHIPFRNSKLTY
LLQYSLTGDSKTLMFVNISPSSSHINETLNSLRFASKVNSTRLVSRK"
gene <818323..>819075
/gene="RRP15"
/locus_tag="YPR143W"
/db_xref="GeneID:856264"
mRNA <818323..>819075
/gene="RRP15"
/locus_tag="YPR143W"
/product="rRNA-processing protein RRP15"
/transcript_id="NM_001184240.1"
/db_xref="GeneID:856264"
CDS 818323..819075
/gene="RRP15"
/locus_tag="YPR143W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0030687
preribosome, large subunit precursor [PMID:15769876]"
/experiment="EXISTENCE:mutant phenotype:GO:0000463
maturation of LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15769876]"
/experiment="EXISTENCE:mutant phenotype:GO:0000466
maturation of 5.8S rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15769876]"
/note="Nucleolar protein; constituent of pre-60S ribosomal
particles; required for proper processing of the 27S
pre-rRNA at the A3 and B1 sites to yield mature 5.8S and
25S rRNAs"
/codon_start=1
/product="rRNA-processing protein RRP15"
/protein_id="NP_015469.1"
/db_xref="GeneID:856264"
/db_xref="SGD:S000006347"
/translation="MGSKHRVDTKDKKRTRKNAEFGREKRNSGNQELSNEPEKDTIME
GDEAEEDEQNSSSDESSKIIDNEQSDAEEDDDEEEEDDDFPRKKKSKNSKHDDGSTGF
SAAVNAILSSHLKAYDRKDPIMARNKKVLKQSESEKLEYKAKKALLAEKKKLLGKARK
TDIIPIASGEDRSENIRKVLEKETALRKIAQKGAVKLFNAILATQVKTEKEVSENLSE
IKNKEEKKELITEVSKEKFLDLVKAAAGSDNE"
rep_origin 819160..819399
/note="ARS1627; Autonomously Replicating Sequence"
/db_xref="SGD:S000118423"
gene 819529..819602
/locus_tag="YNCP0020W"
/db_xref="GeneID:856266"
tRNA 819529..819602
/locus_tag="YNCP0020W"
/product="tRNA-Ile"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Isoleucine tRNA (tRNA-Ile), predicted by
tRNAscan-SE analysis"
/db_xref="GeneID:856266"
/db_xref="SGD:S000006611"
gene complement(<819765..>821423)
/gene="NOC4"
/locus_tag="YPR144C"
/gene_synonym="UTP19"
/db_xref="GeneID:856267"
mRNA complement(<819765..>821423)
/gene="NOC4"
/locus_tag="YPR144C"
/gene_synonym="UTP19"
/product="ribosome biosynthesis protein NOC4"
/transcript_id="NM_001184241.1"
/db_xref="GeneID:856267"
CDS complement(819765..821423)
/gene="NOC4"
/locus_tag="YPR144C"
/gene_synonym="UTP19"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10684247|PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:12150911|PMID:12446671|PMID:15590835]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/experiment="EXISTENCE:direct assay:GO:0032040
small-subunit processome [PMID:15590835]"
/experiment="EXISTENCE:mutant phenotype:GO:0000447
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from
5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15590835]"
/experiment="EXISTENCE:mutant phenotype:GO:0000472
endonucleolytic cleavage to generate mature 5'-end of
SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:15590835]"
/experiment="EXISTENCE:mutant phenotype:GO:0000480
endonucleolytic cleavage in 5'-ETS of tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:15590835]"
/experiment="EXISTENCE:mutant phenotype:GO:0006364 rRNA
processing [PMID:12446671]"
/experiment="EXISTENCE:mutant phenotype:GO:0042274
ribosomal small subunit biogenesis [PMID:12446671]"
/experiment="EXISTENCE:physical interaction:GO:0030692
Noc4p-Nop14p complex [PMID:12446671]"
/note="Nucleolar protein; forms a complex with Nop14p that
mediates maturation and nuclear export of 40S ribosomal
subunits; relocalizes to the cytosol in response to
hypoxia"
/codon_start=1
/product="ribosome biosynthesis protein NOC4"
/protein_id="NP_015470.1"
/db_xref="GeneID:856267"
/db_xref="SGD:S000006348"
/translation="MVLLISEIKDIAKRLTAAGDRKQYNSIIKLINELVIPENVTQLE
EDETEKNLRFLVMSLFQIFRKLFSRGDLTLPSSKKSTLEKEQFVNWCRKVYEAFKTKL
LAIISDIPFETSLGLDSLDVYLQLAELESTHFASEKGAPFFPNKTFRKLIIALWSSNM
GEIEDVKSSGASENLIIVEFTEKYYTKFADIQYYFQSEFNQLLEDPAYQDLLLKNVGK
WLALVNHDKHCSSVDADLEIFVPNPPQAIENESKFKSNFEKNWLSLLNGQLSLQQYKS
ILLILHKRIIPHFHTPTKLMDFLTDSYNLQSSNKNAGVVPILALNGLFELMKRFNLEY
PNFYMKLYQIINPDLMHVKYRARFFRLMDVFLSSTHLSAHLVASFIKKLARLTLESPP
SAIVTVIPFIYNLIRKHPNCMIMLHNPAFISNPFQTPDQVANLKTLKENYVDPFDVHE
SDPELTHALDSSLWELASLMEHYHPNVATLAKIFAQPFKKLSYNMEDFLDWNYDSLLN
AESSRKLKTLPTLEFEAFTNVFDNEDGDSEASSQGNVYLPGVAW"
gene 821732..821903
/gene="SNR45"
/locus_tag="YNCP0021W"
/db_xref="GeneID:9164982"
ncRNA 821732..821903
/ncRNA_class="snoRNA"
/gene="SNR45"
/locus_tag="YNCP0021W"
/product="SNR45"
/experiment="EXISTENCE:direct assay:GO:0070181 small
ribosomal subunit rRNA binding [PMID:28542199]"
/experiment="EXISTENCE:mutant phenotype:GO:1990882 rRNA
acetylation [PMID:28542199]"
/experiment="EXISTENCE:physical interaction:GO:0031428 box
C/D methylation guide snoRNP complex [PMID:11081632]"
/note="C/D box small nucleolar RNA (snoRNA); acts as
antisense guide to target RNA base acetylation by Kre33p
to cytosine 1773 in 18S rRNA (responsible for ac4C 1773);
establishes extended bipartite complementarity around the
cytosines targeted for acetylation, similar to
pseudouridylation pocket formation by the H/ACA snoRNPs;
base pairing between snR45 and 18S rRNA requires the
putative helicase activity of Kre33p"
/transcript_id="NR_132271.1"
/db_xref="GeneID:9164982"
/db_xref="SGD:S000006505"
gene <822620..>824338
/gene="ASN1"
/locus_tag="YPR145W"
/db_xref="GeneID:856268"
mRNA <822620..>824338
/gene="ASN1"
/locus_tag="YPR145W"
/product="asparagine synthase (glutamine-hydrolyzing) 1"
/transcript_id="NM_001184242.1"
/db_xref="GeneID:856268"
CDS 822620..824338
/gene="ASN1"
/locus_tag="YPR145W"
/EC_number="6.3.5.4"
/experiment="EXISTENCE:direct assay:GO:0004066 asparagine
synthase (glutamine-hydrolyzing) activity [PMID:6105958]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0097268
cytoophidium [PMID:39836171]"
/experiment="EXISTENCE:genetic interaction:GO:0070981
L-asparagine biosynthetic process [PMID:8951815]"
/note="Asparagine synthetase; catalyzes the synthesis of
L-asparagine from L-aspartate in the asparagine
biosynthetic pathway; ASN1 has a paralog, ASN2, that arose
from the whole genome duplication"
/codon_start=1
/product="asparagine synthase (glutamine-hydrolyzing) 1"
/protein_id="NP_015471.1"
/db_xref="GeneID:856268"
/db_xref="SGD:S000006349"
/translation="MCGIFAAFRHEDVHRYKPKALQLSKRIRHRGPDWSGNAIKNSTI
FVHERLAIVGVESGAQPITSSDGEYMLCVNGEIYNHIQLREECADYEFGTLSDCEPII
PMYLKHDIDAPKYLDGMFAWTLYDAKQDRIVAARDPIGITTLYMGRSSASPKTVYFAS
ELKCLTDDCDTITAFPPGHVYDSKTDKITRYFTPDWLDEKRIPSTPIDYMAIRHSLEK
AVRKRLMAEVPYGVLLSGGLDSSLIASIAARETAKATNDVEPSTYDSKARHLAGIDDD
GKLHTAGWTSLHSFAIGLPNAPDLQAARKVAKFIGSIHHEHTFTLQEGLDALDDVIYH
LETYDVTTIRASTPMFLLSRKIKAQGVKMVLSGEGSDEIFGGYLYFAQAPSAAEFHTE
SVQRVKNLHLADCLRANKSTMAWGLEARVPFLDREFLQLCMNIDPNEKMIKPKEGRIE
KYILRKAFDTTGEPDAKPYLPEEILWRQKEQFSDGVGYSWIDGLKDTAEAVISDEMFA
SPKAEWGSDIPTTKEAFWYRLKFDALFPQKTVADTVMRWIPKADWGCAEDPSGRYAQI
HEKHIE"
gene complement(<824690..>824926)
/locus_tag="YPR145C-A"
/db_xref="GeneID:3799978"
mRNA complement(<824690..>824926)
/locus_tag="YPR145C-A"
/product="uncharacterized protein"
/transcript_id="NM_001184687.1"
/db_xref="GeneID:3799978"
CDS complement(824690..824926)
/locus_tag="YPR145C-A"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001032572.1"
/db_xref="GeneID:3799978"
/db_xref="SGD:S000113589"
/translation="MSTAFRKIKLIFKKSDSQYPQNYRAEIKSRNKNTVITRHDLLIA
HEMKQRASLERSNSIRNLQSQGKRRSDSKESRKL"
gene complement(<825645..>826559)
/locus_tag="YPR147C"
/db_xref="GeneID:856270"
mRNA complement(<825645..>826559)
/locus_tag="YPR147C"
/product="bifunctional triacylglycerol lipase/ester
hydrolase"
/transcript_id="NM_001184244.1"
/db_xref="GeneID:856270"
CDS complement(825645..826559)
/locus_tag="YPR147C"
/EC_number="3.1.1.3"
/experiment="EXISTENCE:direct assay:GO:0004806
triacylglycerol lipase activity [PMID:29491250]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005811 lipid
droplet [PMID:24868093]"
/experiment="EXISTENCE:direct assay:GO:0016788 hydrolase
activity, acting on ester bonds [PMID:29491250]"
/experiment="EXISTENCE:mutant phenotype:GO:0006629 lipid
metabolic process [PMID:29491250]"
/experiment="EXISTENCE:mutant phenotype:GO:0019433
triglyceride catabolic process [PMID:29491250]"
/note="Bifunctional triacylglycerol lipase and short chain
ester hydrolase; null mutant results in the accumulation
of both triacylglycerol and fatty acids derived from
neutral lipids and phospholipids as well as an increase in
the quantity of lipid droplets; contains an alpha/beta
hydrolase domain with a conserved GXSXG lipase motif;
localizes to lipid droplets; GFP-fusion protein localizes
to the cytoplasm and is induced in response to the
DNA-damaging agent MMS"
/codon_start=1
/product="bifunctional triacylglycerol lipase/ester
hydrolase"
/protein_id="NP_015473.1"
/db_xref="GeneID:856270"
/db_xref="SGD:S000006351"
/translation="MTVKEYTKSKLPCSILNIKPTVTKSGEDAPLLVWIPGNPGLLYY
YQEMLHHLHLKHPDWEILGISHAGMTLNAHSNTPIFSLQDQVDHQVEVINNFSCKNRK
IIIMGHSVGAYIVQKVCLSNKLVGSVQKVGLVTPTVMDIHTSEMGIKMTAALRYIPPL
AHVVSLFSYIFFYWILSEGFSRFIIDKFMGCGSTGYQAVLSTRIFLTHRQFVRQSLGL
AAQEMEEITTNWEFQDRFINYCEENGISIWFLFSSNDHWVSGKTRSHLSDYYKDKVKQ
ERLKIDVTDKIPHSFVVKHAEYAINAFF"
gene complement(<826833..>828140)
/locus_tag="YPR148C"
/db_xref="GeneID:856271"
mRNA complement(<826833..>828140)
/locus_tag="YPR148C"
/product="uncharacterized protein"
/transcript_id="NM_001184245.1"
/db_xref="GeneID:856271"
CDS complement(826833..828140)
/locus_tag="YPR148C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="hypothetical protein; may interact with ribosomes,
based on co-purification experiments; green fluorescent
protein (GFP)-fusion protein localizes to the cytoplasm in
a punctate pattern"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015474.1"
/db_xref="GeneID:856271"
/db_xref="SGD:S000006352"
/translation="MSGYFSGFSLNKITDSIATAAHKTQDTLNNALANANVNLNDPQT
RLSIKSRTRFVQESLGTVSDISKLPPQYQFLEKKSDSLEKVCKRILLVSKTFEVEGYD
YPPNLTESISDWWSLNKDGWFGSKKSESSTKKKGSNHDDAFLPRSFAQAISKAAVDCE
CEFQNLEHNEKAELKKKKESIKTAQTTEAQGADHNEEDEEDEEDEEDDEDLSNLIKVF
DSWSTCYKNIDEGKAEMDSMMVKEFNKKLETLINQDFKKVHDLRKKVEESRLKFDTMR
YEVKAKEAELEAKKAEATGEAHSKDVSAKDISANTTTSFDETPSTEDEKPKSEGAEEE
SKKEANEPTVDDVADRKEDLKSNKVNDEPPIEESEDNKLLEKLEDEFVSNTTAAVETM
EEITDSSEILGLIKLFQNFQLVYFRQCVQEVEANLKVLNGLEI"
gene <829918..>830439
/gene="NCE102"
/locus_tag="YPR149W"
/gene_synonym="NCE2"
/db_xref="GeneID:856272"
mRNA <829918..>830439
/gene="NCE102"
/locus_tag="YPR149W"
/gene_synonym="NCE2"
/product="Nce102p"
/transcript_id="NM_001184246.1"
/db_xref="GeneID:856272"
CDS 829918..830439
/gene="NCE102"
/locus_tag="YPR149W"
/gene_synonym="NCE2"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836|PMID:12469340]"
/experiment="EXISTENCE:direct assay:GO:0033101 cellular
bud membrane [PMID:19064668]"
/experiment="EXISTENCE:direct assay:GO:0070250 mating
projection membrane [PMID:19064668]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0061091
regulation of phospholipid translocation [PMID:27033517]"
/experiment="EXISTENCE:mutant phenotype:GO:0001933
negative regulation of protein phosphorylation
[PMID:19564405]"
/experiment="EXISTENCE:mutant phenotype:GO:0007009 plasma
membrane organization [PMID:19564405]"
/experiment="EXISTENCE:mutant phenotype:GO:0009306 protein
secretion [PMID:8655575]"
/experiment="EXISTENCE:mutant phenotype:GO:0060237
regulation of fungal-type cell wall organization
[PMID:27033517]"
/experiment="EXISTENCE:mutant phenotype:GO:0070941
eisosome assembly [PMID:38096056|PMID:19564405]"
/experiment="EXISTENCE:mutant phenotype:GO:0072659 protein
localization to plasma membrane [PMID:20581291]"
/experiment="EXISTENCE:mutant phenotype:GO:0097576 vacuole
fusion [PMID:33114062]"
/note="Protein involved in regulation of pheromone
response and mating; contains transmembrane domains;
involved in secretion of proteins that lack classical
secretory signal sequences; component of the
detergent-insoluble glycolipid-enriched complexes (DIGs);
NCE102 has a paralog, FHN1, that arose from the whole
genome duplication"
/codon_start=1
/product="Nce102p"
/protein_id="NP_015475.1"
/db_xref="GeneID:856272"
/db_xref="SGD:S000006353"
/translation="MLALADNILRIINFLFLVISIGLISSLLNTQHRHSSRVNYCMFA
CAYGIFTDSLYGVFANFIEPLAWPLVLFTLDFLNFVFTFTAGTVLAVGIRAHSCNNSS
YVDSNKITQGSGTRCRQAQAAVAFLYFSCAIFLAKTLMSVFNMISNGAFGSGSFSKRR
RTGQVGVPTISQV"
gene complement(<831055..>831675)
/gene="SUE1"
/locus_tag="YPR151C"
/db_xref="GeneID:856274"
mRNA complement(<831055..>831675)
/gene="SUE1"
/locus_tag="YPR151C"
/product="Sue1p"
/transcript_id="NM_001184248.1"
/db_xref="GeneID:856274"
CDS complement(831055..831675)
/gene="SUE1"
/locus_tag="YPR151C"
/experiment="EXISTENCE:direct assay:GO:0005740
mitochondrial envelope [PMID:15123691]"
/experiment="EXISTENCE:mutant phenotype:GO:0030163 protein
catabolic process [PMID:15123691]"
/note="Protein required for degradation of unstable forms
of cytochrome c; located in the mitochondria"
/codon_start=1
/product="Sue1p"
/protein_id="NP_015477.1"
/db_xref="GeneID:856274"
/db_xref="SGD:S000006355"
/translation="MILLKRTKIRGVSVSFVSLQRRTHSRLVNPIRQQHQQITKQRSS
KILKNAHFYDFRSLPKVPTTQYLEARELTRDILYSGYRPVMYPVKENPLFRDKKRKSL
QTLLTMNEKTNAEAKTIDEKKHKNILFGERGTGGIMSGGVNGTWKYNPTVPNELLPFN
WWSTSSMGMEYFPEWKNVPPYMMRKLKPFDKALQMRLTHKSKKKMK"
gene complement(<832061..>833458)
/gene="URN1"
/locus_tag="YPR152C"
/db_xref="GeneID:856275"
mRNA complement(<832061..>833458)
/gene="URN1"
/locus_tag="YPR152C"
/product="Urn1p"
/transcript_id="NM_001184249.1"
/db_xref="GeneID:856275"
CDS complement(832061..833458)
/gene="URN1"
/locus_tag="YPR152C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0071010
prespliceosome [PMID:19941820]"
/note="hypothetical protein containing WW and FF domains;
overexpression causes accumulation of cells in G1 phase"
/codon_start=1
/product="Urn1p"
/protein_id="NP_015478.1"
/db_xref="GeneID:856275"
/db_xref="SGD:S000006356"
/translation="MRGEWQEFKTPAGKKYYYNKNTKQSRWEKPNLKKGSNLESNAKE
SQTERKPTFSLELVNGWHLIIYNDGTKLYFNDDSKEFKNDISQEDDSRCRSLIESLDK
EKLVLLIGVARGYTMREEDIDKILESCNEEIHLFKRNQDEVERKDEISEEAGDVKSPL
QESHTGLVSGYGSSSGEEDEEEDEEEDEENEEQIVNQDISIIDDLNRIDTDDIDERNI
FFELFDRYKLDKFSTWSLQSKKIENDPDFYKIRDDTVRESLFEEWCGERSGNATAEES
DSEDNSEDDSEVLEPTKYHYLAQIVANAGTIAPDTIPQDIRKQQKALYKAYKIKEYIP
SKRDQDKFVSQLLFYYKTFDLEQRKEIFCDCLRDHERDFTGAVESLRQDKELIDRWQT
LLKAPADSSSIEDILLSIEHRCCVSPIVVTEPRYYVVGILEKTVVWVRWLAAEVGPSS
RFTPVGAGNEPINPE"
gene <833689..>834245
/gene="MAY24"
/locus_tag="YPR153W"
/gene_synonym="EHG1"
/db_xref="GeneID:856276"
mRNA join(<833689..833693,833828..>834245)
/gene="MAY24"
/locus_tag="YPR153W"
/gene_synonym="EHG1"
/product="May24p"
/transcript_id="NM_001184250.1"
/db_xref="GeneID:856276"
CDS join(833689..833693,833828..834245)
/gene="MAY24"
/locus_tag="YPR153W"
/gene_synonym="EHG1"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:31797992]"
/experiment="EXISTENCE:direct assay:GO:0032541 cortical
endoplasmic reticulum [PMID:40601413]"
/experiment="EXISTENCE:direct assay:GO:0044860 protein
localization to plasma membrane raft [PMID:40601413]"
/experiment="EXISTENCE:direct assay:GO:0071464 cellular
response to hydrostatic pressure [PMID:40601413]"
/experiment="EXISTENCE:mutant phenotype:GO:0071464
cellular response to hydrostatic pressure [PMID:31797992]"
/note="Peripheral ER membrane protein; putative ER
chaperone required for the stability and function of
nutrient permeases exposed to high pressure"
/codon_start=1
/product="May24p"
/protein_id="NP_015479.2"
/db_xref="GeneID:856276"
/db_xref="SGD:S000006357"
/translation="MRSFVTNNDIPVGYVTPKFPSLYWPINNSKYNTAFLYYISDIWK
FSLYWTLIFNGAFYVTAGVYASLTHRKKAGSVWIFVMYVLYGGVQGLTTGTVMGFLIG
AIYRSGLFSMSTWVPLCCAVVQILFDVVLSYSMVGSVM"
gene <834565..>835212
/gene="PIN3"
/locus_tag="YPR154W"
/gene_synonym="LSB2"
/db_xref="GeneID:856277"
mRNA <834565..>835212
/gene="PIN3"
/locus_tag="YPR154W"
/gene_synonym="LSB2"
/product="Pin3p"
/transcript_id="NM_001184251.1"
/db_xref="GeneID:856277"
CDS 834565..835212
/gene="PIN3"
/locus_tag="YPR154W"
/gene_synonym="LSB2"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:23577202|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0030479 actin
cortical patch [PMID:23577202]"
/experiment="EXISTENCE:direct assay:GO:0034316 negative
regulation of Arp2/3 complex-mediated actin nucleation
[PMID:23577202]"
/experiment="EXISTENCE:physical interaction:GO:0034316
negative regulation of Arp2/3 complex-mediated actin
nucleation [PMID:23577202]"
/note="Negative regulator of actin nucleation-promoting
factor activity; interacts with Las17p, a homolog of human
Wiskott-Aldrich Syndrome protein (WASP), via an N-terminal
SH3 domain, and along with LSB1 cooperatively inhibits the
nucleation of actin filaments; short-lived protein whose
levels increase in response to thermal stress; induces the
formation of the [PIN+] and [RNQ+] prions when
overproduced; PIN3 has a paralog, LSB1, that arose from
the whole genome duplication"
/codon_start=1
/product="Pin3p"
/protein_id="NP_015480.1"
/db_xref="GeneID:856277"
/db_xref="SGD:S000006358"
/translation="MSASLINRSLTNIRTELDFLKGSNVISNDVYDQINKSLPAKWDP
ANAPRNASPASLEYVEALYQFDPQQDGDLGLKPGDKVQLLEKLSPEWYKGSCNGRTGI
FPANYVKPAFSGSNGPSNLPPPPQYKAQELQQIPTQNSAASSYQQQPFPPPSTNYYQQ
PQQQPQQAPPPQQQQQQQQHQSSHSHLKSFGSKLGNAAIFGAGASIGSDIVNNIF"
gene complement(<835563..>837413)
/gene="NCA2"
/locus_tag="YPR155C"
/db_xref="GeneID:856278"
mRNA complement(<835563..>837413)
/gene="NCA2"
/locus_tag="YPR155C"
/product="Nca2p"
/transcript_id="NM_001184252.1"
/db_xref="GeneID:856278"
CDS complement(835563..837413)
/gene="NCA2"
/locus_tag="YPR155C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:14562095|PMID:16823961|PMID:14576278|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:16407407]"
/experiment="EXISTENCE:mutant phenotype:GO:0009060 aerobic
respiration [PMID:7723016]"
/experiment="EXISTENCE:mutant phenotype:GO:0016071 mRNA
metabolic process [PMID:7723016]"
/note="Protein that regulates expression of Fo-F1 ATP
synthase subunits; involved in the regulation of
mitochondrial expression of subunits 6 (Atp6p) and 8
(Atp8p) of the Fo-F1 ATP synthase; functions with Nca3p"
/codon_start=1
/product="Nca2p"
/protein_id="NP_015481.1"
/db_xref="GeneID:856278"
/db_xref="SGD:S000006359"
/translation="MIINRRILKSFEEISHSLEESLREVAFDSQQQLIQDVREENEEL
SRLQDQLQLIRSIVEKICISIKTDNIDSYCSVPFDLLYNICKDIADPSSFEDGDLQYL
VSQAIFEYIILLCYYSVTNECVQGLPAVYEAEQYYKTVSDSILKSFLYCLQNSVSTIR
LLSQTVLKDVNKKKLSHQKWSLKALSVDLLEKIRPRINKFMVIRNFRFVGLPKKPIEI
ASLVSDIPRGIVHERLDMVTQSSKYYTIKLGQLITEFDQQPEENGMFTEVHLPNYERR
LKSLQDFFGLAMSDSNLLDVIRCSAKFHKDHPLRRFTKPSILTRYWPSILLCLLYGPS
SVMSLWNSRYFIQDFIKTNVVDFAKGLILNWLWAPLKQVWSTVKHDEGSAISVTSQET
LNSDMDSLTRMIVSFVVDNSDSTSNSPIDPILLSTKVEHGDLTEFMEIYETQLHHPIK
NIATGGLVRSLLIQLQKTKVDGSMALNGIDKMLKSQQLVFGVVALSPALVILYSSIVA
LKRFVKLGNVWSNEKRYREQISISLNNVERVLNYSKQGADADEEHLNQGLLVIEVSNL
YKLGSFLIPRSRKKEWFRDVEELVDTNLDSGAHINVVNRIYHVYGRFLIH"
gene complement(<837909..>839777)
/gene="TPO3"
/locus_tag="YPR156C"
/db_xref="GeneID:856279"
mRNA complement(<837909..>839777)
/gene="TPO3"
/locus_tag="YPR156C"
/product="spermine transporter"
/transcript_id="NM_001184253.1"
/db_xref="GeneID:856279"
CDS complement(837909..839777)
/gene="TPO3"
/locus_tag="YPR156C"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:12562762]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0000296
spermine transport [PMID:11171066]"
/experiment="EXISTENCE:mutant phenotype:GO:0000297
spermine transmembrane transporter activity
[PMID:11171066]"
/experiment="EXISTENCE:mutant phenotype:GO:0000329
fungal-type vacuole membrane [PMID:11171066]"
/note="Polyamine transporter of major facilitator
superfamily; member of 12-spanner drug:H(+) antiporter
DHA1 family; specific for spermine; localizes to plasma
membrane; targeted to vacuole via AP-3 pathway; TPO3 has a
paralog, TPO2, that arose from the whole genome
duplication"
/codon_start=1
/product="spermine transporter"
/protein_id="NP_015482.1"
/db_xref="GeneID:856279"
/db_xref="SGD:S000006360"
/translation="MNRQESINSFNSDETSSLSDVESQQPQQYIPSESGSKSNMAPNQ
LKLTRTETVKSLQDMGVSSKAPVPDVNAPQSSKNKIFPEEYTLETPTGLVPVATLHSI
GRTSTAISRTRTRQIDGASSPSSNEDALESDNNEKGKEGDSSGANDEAPDLDPEIEFV
TFVTGDPENPHNWPAWIRWSYTVLLSILVICVAYGSACISGGLGTVEKKYHVGMEAAI
LSVSLMVIGFSLGPLIWSPVSDLYGRRVAYFVSMGLYVIFNIPCALAPNLGSLLACRF
LCGVWSSSGLCLVGGSIADMFPSETRGKAIAFFAFAPYVGPVVGPLVNGFISVSTGRM
DLIFWVNMAFAGVMWIISSAIPETYAPVILKRKAARLRKETGNPKIMTEQEAQGVSMG
EMMRACLLRPLYFSVTEPVLVATCFYVCLIYSLLYAFFFAFPVIFGELYGYKDNLVGL
MFIPIVIGALWALATTFYCENKYLQIVKQRKPTPEDRLLGAKIGAPFAAIALWILGAT
AYKHIIWVGPASAGLAFGFGMVLIYYSLNNYIIDCYVQYASSALATKVFLRSAGGAAF
PLFTIQMYHKLNLHWGSWLLAFISTAMIALPFAFSYWGKGLRHKLSKKDYSIDSIE"
gene <841266..>842669
/gene="TDA6"
/locus_tag="YPR157W"
/db_xref="GeneID:856280"
mRNA <841266..>842669
/gene="TDA6"
/locus_tag="YPR157W"
/product="Tda6p"
/transcript_id="NM_001184254.1"
/db_xref="GeneID:856280"
CDS 841266..842669
/gene="TDA6"
/locus_tag="YPR157W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="hypothetical protein; induced by treatment with
8-methoxypsoralen and UVA irradiation; null mutant is
sensitive to expression of the top1-T722A allele; SWAT-GFP
and mCherry fusion proteins localize to the cell periphery
and vacuole; TDA6 has a paralog, VPS62, that arose from
the whole genome duplication"
/codon_start=1
/product="Tda6p"
/protein_id="NP_015483.1"
/db_xref="GeneID:856280"
/db_xref="SGD:S000006361"
/translation="MHCVLARILLWFLIVDLSVIRALVLPPLKDYDPLEPLMKRDMAM
GQRNRFKVDGQLPPILNSTDVTDDQRSLHTPGEIPSYVINHCPLVHLYSEEKYWPSDI
AEYVQNFQIKDKNGNSISTHENLTLHDLKAEYHVDLFGNKTETHIPSSEVFLTSLDDF
DKDPKWLLGHLPEYGTGYNSKAPAILIVVDKGNGWVDAFWFFFYPFNHGPFIMGHGPW
GNHVGDWEHSLVRFYKGIPKYLWMSAHSSGTGYRYEAVEKFKKLRKRKQQDSDDGGDT
ILERPLIFSARGTHANYASAGQHAHDIPFFFMPLSDFTDRGPLWDPSLNFYSYTFDGK
TVTPSSEREESLGLDWLHFQGGWGDQQLPARDPRQKWCVAQWKYIGGPRGPLFKKLDR
LNLCGGVKKWNFWNGGCPARRLIKKAEGLDSESTDLMGDNCGVLLYRIRPKWLRGILR
FLMWRGILCSLMEFFTN"
rep_origin 842653..842900
/note="ARS1628; Autonomously Replicating Sequence"
/db_xref="SGD:S000118424"
gene <843262..>844020
/gene="CUR1"
/locus_tag="YPR158W"
/db_xref="GeneID:856281"
mRNA <843262..>844020
/gene="CUR1"
/locus_tag="YPR158W"
/product="Cur1p"
/transcript_id="NM_001184255.1"
/db_xref="GeneID:856281"
CDS 843262..844020
/gene="CUR1"
/locus_tag="YPR158W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22718905|PMID:18833194]"
/experiment="EXISTENCE:genetic interaction:GO:0006457
protein folding [PMID:22718905]"
/experiment="EXISTENCE:mutant phenotype:GO:0006457 protein
folding [PMID:22718905]"
/experiment="EXISTENCE:mutant phenotype:GO:0006886
intracellular protein transport [PMID:22718905]"
/experiment="EXISTENCE:mutant phenotype:GO:0034504 protein
localization to nucleus [PMID:22718905]"
/experiment="EXISTENCE:physical interaction:GO:0006457
protein folding [PMID:22718905]"
/experiment="EXISTENCE:physical interaction:GO:0006886
intracellular protein transport [PMID:22718905]"
/experiment="EXISTENCE:physical interaction:GO:0034504
protein localization to nucleus [PMID:22718905]"
/experiment="EXISTENCE:physical interaction:GO:0051087
protein-folding chaperone binding [PMID:22718905]"
/note="Sorting factor, central regulator of spatial
protein quality control; physically and functionally
interacts with chaperones to promote sorting and
deposition of misfolded proteins into cytosolic
compartments; involved in destabilization of [URE3]
prions; CUR1 has a paralog, BTN2, that arose from the
whole genome duplication"
/codon_start=1
/product="Cur1p"
/protein_id="NP_015484.1"
/db_xref="GeneID:856281"
/db_xref="SGD:S000006362"
/translation="MAAACICQPNLLEINVSDGPLDMIRKKRKIQQPQLRPPLRENKC
QPHFSVRKVNQSYIISLHKEITCQLIAEIVKQKLSRIWEKVYIPSYELISDKDGNQIY
VEQSVDENRLTSEIMEKLDPNNIDIEAIEILFDDYHLELSRLTNGIIISSANDHFYRE
FSFNNIIDDNFKICGTSMSADSFDKIYGVMWIEVPFNGNGLQNDSAVNRVSTSHNQIE
ELNDIEQEIRAFNISRSNQESIIKKEVSRRLNGR"
mobile_element 844412..850340
/note="YPRWTy1-3; Ty1 element, LTR retrotransposon of the
Copia (Pseudoviridae) group; contains co-transcribed genes
TYA Gag and TYB Pol, encoding proteins involved in
structure and function of virus-like particles, flanked by
two direct repeats"
/mobile_element_type="retrotransposon:YPRWTy1-3"
/db_xref="SGD:S000007220"
repeat_region 844412..844749
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007214"
gene <844709..>849980
/locus_tag="YPR158W-B"
/db_xref="GeneID:856283"
mRNA <844709..>849980
/locus_tag="YPR158W-B"
/product="gag-pol fusion protein"
/transcript_id="NM_001184398.2"
/db_xref="GeneID:856283"
CDS join(844709..846013,846015..849980)
/locus_tag="YPR158W-B"
/EC_number="2.7.7.7"
/EC_number="2.7.7.49"
/EC_number="3.1.26.4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9448009]"
/ribosomal_slippage
/note="Retrotransposon TYA Gag and TYB Pol genes;
transcribed/translated as one unit; polyprotein is
processed to make a nucleocapsid-like protein (Gag),
reverse transcriptase (RT), protease (PR), and integrase
(IN); similar to retroviral genes"
/codon_start=1
/product="gag-pol fusion protein"
/protein_id="NP_058193.1"
/db_xref="GeneID:856283"
/db_xref="SGD:S000007364"
/translation="MESQQLSNYPHISHGSACASVTSKEVHTNQDPLDVSASKIQEYD
KASTKANSQQTTTPASSAVPENPHHASPQPASVPPPQNGPYPQQCMMTQNQANPSGWS
FYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVRPITDDELT
FLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQ
YNGSTPADAFETKVTNIIDRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNM
TVAELFLDIHAIYEEQQGSRNSKPNYRRNPSDEKNDSRSYTNTTKPKVIARNPQKTNN
SKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLGQKLTESTVNHTNHS
DDELPGHLLLDSGASRTLIRSAHHIHSASSNPDINVVDAQKRNIPINAIGDLQFHFQD
NTKTSIKVLHTPNIAYDLLSLNELAAVDITACFTKNVLERSDGTVLAPIVKYGDFYWV
SKKYLLPSNISVPTINNVHTSESTRKYPYPFIHRMLAHANAQTIRYSLKNNTITYFNE
SDVDWSSAIDYQCPDCLIGKSTKHRHIKGSRLKYQNSYEPFQYLHTDIFGPVHNLPKS
APSYFISFTDETTKFRWVYPLHDRREDSILDVFTTILAFIKNQFQASVLVIQMDRGSE
YTNRTLHKFLEKKNGITPCYTTTADSRAHGVAERLNRTLLDDCRTQLQCSGLPNHLWF
SAIEFSTIVRNSLASPKSKKSARQHAGLAGLDISTLLPFGQPVIVNDHNPNSKIHPRG
IPGYALHPSRNSYGYIIYLPSLKKTVDTTNYVILQGKESRLDQFNYDALTFDEDLNRL
TASYHSFIASNEIQESNDLNIESDHDFQSDIELHPEQPRNVLSKAVSPTDSTPPSTHT
EDSKRVSKTNIRAPREVDPNISESNILPSKKRSSTPQISNIESTGSGGMHKLNVPLLA
PMSQSNTHESSHASKSKDFRHSDSYSENETNHTNVPISSTGGTNNKTVPQISDQETEK
RIIHRSPSIDASPPENNSSHNIVPIKTPTTVSEQNTEESIIADLPLPDLPPESPTEFP
DPFKELPPINSHQTNSSLGGIGDSNAYTTINSKKRSLEDNETEIKVSRDTWNTKNMRS
LEPPRSKKRIHLIAAVKAVKSIKPIRTTLRYDEAITYNKDIKEKEKYIEAYHKEVNQL
LKMNTWDTDKYYDRKEIDPKRVINSMFIFNRKRDGTHKARFVARGDIQHPDTYDSGMQ
SNTVHHYALMTSLSLALDNNYYITQLDISSAYLYADIKEELYIRPPPHLGMNDKLIRL
KKSLYGLKQSGANWYETIKSYLIKQCGMEEVRGWSCVFKNSQVTICLFVDDMILFSKD
LNANKKIITTLKKQYDTKIINLGESDNEIQYDILGLEIKYQRGKYMKLGMENSLTEKI
PKLNVPLNPKGRKLSAPGQPGLYIDQDELEIDEDEYKEKVHEMQKLIGLASYVGYKFR
FDLLYYINTLAQHILFPSRQVLDMTYELIQFMWDTRDKQLIWHKNKPTEPDNKLVAIS
DASYGNQPYYKSQIGNIYLLNGKVIGGKSTKASLTCTSTTEAEIHAISESVPLLNNLS
HLVQELNKKPITKGLLTDSKSTISIIISNNEEKFRNRFFGTKAMRLRDEVSGNHLHVC
YIETKKNIADVMTKPLPIKTFKLLTNKWIH"
gene <844709..>846031
/locus_tag="YPR158W-A"
/db_xref="GeneID:856282"
mRNA <844709..>846031
/locus_tag="YPR158W-A"
/product="gag protein"
/transcript_id="NM_001184397.1"
/db_xref="GeneID:856282"
CDS 844709..846031
/locus_tag="YPR158W-A"
/note="Retrotransposon TYA Gag gene co-transcribed with
TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is
a nucleocapsid protein that is the structural constituent
of virus-like particles (VLPs); similar to retroviral Gag"
/codon_start=1
/product="gag protein"
/protein_id="NP_058194.1"
/db_xref="GeneID:856282"
/db_xref="SGD:S000007363"
/translation="MESQQLSNYPHISHGSACASVTSKEVHTNQDPLDVSASKIQEYD
KASTKANSQQTTTPASSAVPENPHHASPQPASVPPPQNGPYPQQCMMTQNQANPSGWS
FYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVRPITDDELT
FLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQ
YNGSTPADAFETKVTNIIDRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNM
TVAELFLDIHAIYEEQQGSRNSKPNYRRNPSDEKNDSRSYTNTTKPKVIARNPQKTNN
SKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLRPETY"
repeat_region 850003..850340
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007215"
repeat_region complement(850357..850512)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007204"
rep_origin 850512..850629
/note="ARS1629; Putative replication origin; identified in
multiple array studies, not yet confirmed by plasmid-based
assay"
/db_xref="SGD:S000130201"
mobile_element complement(850629..856554)
/note="YPRCTy1-4; Ty1 element, LTR retrotransposon of the
Copia (Pseudoviridae) group; contains co-transcribed genes
TYA Gag and TYB Pol, encoding proteins involved in
structure and function of virus-like particles, flanked by
two direct repeats"
/mobile_element_type="retrotransposon:YPRCTy1-4"
/db_xref="SGD:S000007209"
repeat_region complement(850629..850966)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007205"
gene complement(<850989..>856257)
/locus_tag="YPR158C-D"
/db_xref="GeneID:856284"
mRNA complement(<850989..>856257)
/locus_tag="YPR158C-D"
/product="gag-pol fusion protein"
/transcript_id="NM_001184396.2"
/db_xref="GeneID:856284"
CDS complement(join(850989..854951,854953..856257))
/locus_tag="YPR158C-D"
/EC_number="2.7.7.7"
/EC_number="2.7.7.49"
/EC_number="3.1.26.4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9448009]"
/ribosomal_slippage
/note="Retrotransposon TYA Gag and TYB Pol genes;
transcribed/translated as one unit; polyprotein is
processed to make a nucleocapsid-like protein (Gag),
reverse transcriptase (RT), protease (PR), and integrase
(IN); similar to retroviral genes"
/codon_start=1
/product="gag-pol fusion protein"
/protein_id="NP_058195.1"
/db_xref="GeneID:856284"
/db_xref="SGD:S000007362"
/translation="MESQQLSNYSPISHGSACASVTSKEVHTNQDPLDVSASKTEECE
KASTKANSQQTTTPASSAVPENPHHASPQPASVPPPQNGPYPQQCMMTQNQANPSGWS
FYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVRQITDDELT
FLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQ
YNGSTPADAFETKVTNIIDRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNM
TVAELFLDIHAIYEEQQGSRNSKPNYRRNLSDEKNDSRSYTNTTKPKVIARNPQKTNN
SKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLGQELTESTVNHTNHS
DDELPGHLLLDSGASRTLIRSAHHIHSASSNPDINVVDAQKRNIPINAIGDLQFHFQD
NTKTSIKVLHTPNIAYDLLSLNELAAVDITACFTKNVLERSDGTVLAPIVQYGDFYWV
SKRYLLPSNISVPTINNVHTSESTRKYPYPFIHRMLAHANAQTIRYSLKNNTITYFNE
SDVDWSSAIDYQCPDCLIGKSTKHRHIKGSRLKYQNSYEPFQYLHTDIFGPVHNLPKS
APSYFISFTDETTKFRWVYPLHDRREDSILDVFTTILAFIKNQFQASVLVIQMDRGSE
YTNRTLHKFLEKNGITPCYTTTADSRAHGVAERLNRTLLDDCRTQLQCSGLPNHLWFS
AIEFSTIVRNSLASPKSKKSASQHAGLAGLDISTLLPFGQPVIVNDHNPNSKIHPRGI
PGYALHPSRNSYGYIIYLPSLKKTVDTTNYVILQGKESRLDQFNYDALTFDEDLNRLT
ASYHSFIASNEIQESNDLNIESDHDFQSDIELHPEQPRNVLSKAVSPTDSTPPSTHTE
DSKPISEINLRAPREVDPNISESNILPSKKRSSTPQISNIESTGSGGMHKLNVPLLAP
MSQSNTHESSHASKSKDFRHSDSYSNNETNHTNVPISSTGGTNNKTVPQISDQETEKR
IIHRSPSIDASPPENNSSHNIVPIKTPTTVSEQNTEESIIADLPLPDPPPEPPTELSD
SFKELPPINSRQTNSSLGGIGDSNAYTTINSKKRSLEDNETEIKVSRDTWNTKNMRSL
EPPRSKKRIHLIAAVKAVKSIKPIRTTLRYDEAITYNKDIKEKEKYIEAYHKEVNQLL
KMKTWDTDRYYDRKEIDPKRVINSMFIFNKKRDGTHKARFVARGDIQHPDTYDTGMQS
NTVHHYALMTSLSLALDNNYYITQLDISSAYLYADIKEELYIRPPPHLGMNDKLIRLK
KSHYGLKQSGANWYETIKSYLIKQCGMEEVRGWSCVFKNSQVTICLFVDDMILFSKDL
NANKKIITTLKKQYDTKIINLGESDNEIQYDILGLEIKYQRGKYMKLGMENSLTEKIP
KLNVPLNPKGRKLSAPGQPGLYIDQDELEIDEDEYKEKVHEMQKLIGLASYVGYKFRF
DLLYYINTLAQHILFPSRQVLDMTYELIQFMWDTRDKQLIWHKNKPTEPDNKLVAISD
ASYGNQPYYKSQIGNIYLLNGKVIGGKSTKASLTCTSTTEAEIHAISESVPLLNNLSH
LVQELNKKPITKGLLTDSKSTISIIISNNEEKFRNRFFGTKAMRLRDEVSGNHLHVCY
IETKKNIADVMTKPLPIKTFKLLTNKWIH"
gene complement(<854935..>856257)
/locus_tag="YPR158C-C"
/db_xref="GeneID:856285"
mRNA complement(<854935..>856257)
/locus_tag="YPR158C-C"
/product="gag protein"
/transcript_id="NM_001184395.1"
/db_xref="GeneID:856285"
CDS complement(854935..856257)
/locus_tag="YPR158C-C"
/note="Retrotransposon TYA Gag gene co-transcribed with
TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is
a nucleocapsid protein that is the structural constituent
of virus-like particles (VLPs); similar to retroviral Gag"
/codon_start=1
/product="gag protein"
/protein_id="NP_058196.1"
/db_xref="GeneID:856285"
/db_xref="SGD:S000007361"
/translation="MESQQLSNYSPISHGSACASVTSKEVHTNQDPLDVSASKTEECE
KASTKANSQQTTTPASSAVPENPHHASPQPASVPPPQNGPYPQQCMMTQNQANPSGWS
FYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVRQITDDELT
FLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQ
YNGSTPADAFETKVTNIIDRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNM
TVAELFLDIHAIYEEQQGSRNSKPNYRRNLSDEKNDSRSYTNTTKPKVIARNPQKTNN
SKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLRPGTY"
repeat_region complement(856217..856554)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007206"
repeat_region 856545..856885
/note="Ty3 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007217"
gene 856902..856974
/locus_tag="YNCP0022W"
/db_xref="GeneID:856286"
tRNA 856902..856974
/locus_tag="YNCP0022W"
/product="tRNA-Ala"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE
analysis; one of 11 nuclear tRNA genes containing the
tDNA-anticodon AGC (converted to IGC in the mature tRNA),
decodes GCU and GCC codons into alanine, one of 16 nuclear
tRNAs for alanine"
/db_xref="GeneID:856286"
/db_xref="SGD:S000006520"
gene <857583..>859745
/gene="KRE6"
/locus_tag="YPR159W"
/gene_synonym="CWH48"
/db_xref="GeneID:856287"
mRNA <857583..>859745
/gene="KRE6"
/locus_tag="YPR159W"
/gene_synonym="CWH48"
/product="beta-glucan synthesis-associated protein KRE6"
/transcript_id="NM_001184256.1"
/db_xref="GeneID:856287"
CDS 857583..859745
/gene="KRE6"
/locus_tag="YPR159W"
/gene_synonym="CWH48"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:17893149]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:21193403]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:7929594]"
/experiment="EXISTENCE:direct assay:GO:0030133 transport
vesicle [PMID:21193403]"
/experiment="EXISTENCE:direct assay:GO:0030427 site of
polarized growth [PMID:21193403]"
/experiment="EXISTENCE:mutant phenotype:GO:0006078
(1->6)-beta-D-glucan biosynthetic process [PMID:7929594]"
/experiment="EXISTENCE:mutant phenotype:GO:0015926
glucosidase activity [PMID:10601196]"
/experiment="EXISTENCE:mutant phenotype:GO:0031505
fungal-type cell wall organization [PMID:7929594]"
/note="Glucosyl hydrolase required for beta-1,6-glucan
biosynthesis; type II integral membrane protein that
localizes to ER, plasma membrane, sites of polarized
growth and secretory vesicles; functionally redundant with
Skn1p; KRE6 has a paralog, SKN1, that arose from the whole
genome duplication"
/codon_start=1
/product="beta-glucan synthesis-associated protein KRE6"
/protein_id="NP_015485.1"
/db_xref="GeneID:856287"
/db_xref="SGD:S000006363"
/translation="MPLRNLTETHNFSSTNLDTDGTGDDHDGAPLSSSPSFGQQNDNS
TNDNAGLTNPFMGSDEESNARDGESLSSSVHYQPQGSDSSLLHDNSRLDLSQNKGVSD
YKGYYSRNNSRAVSTANDNSFLQPPHRAIASSPSLNSNLSKNDILSPPEFDRYPLVGS
RVTSMTQLNHHGRSPTSSPGNESSASFSSNPFLGEQDFSPFGGYPASSFPLMIDEKEE
DDYLHNPDPEEEARLDRRRFIDDFKYMDKRSASGLAGVLLLFLAAIFIFIVLPALTFT
GAIDHESNTEEVTYLTQYQYPQLSAIRTSLVDPDTPDTAKTREAMDGSKWELVFSDEF
NAEGRTFYDGDDPYWTAPDVHYDATKDLEWYSPDASTTVNGTLQLRMDAFKNHGLYYR
SGMLQSWNKVCFTQGALEISANLPNYGRVSGLWPGLWTMGNLGRPGYLASTQGVWPYS
YESCDAGITPNQSSPDGISYLPGQKLSICTCDGEDHPNQGVGRGAPEIDVLEGETDTK
IGVGIASQSLQIAPFDIWYMPDYDFIEVYNFTTTTMNTYAGGPFQQAVSAVSTLNVTW
YEFGEYGGYFQKYAIEYLNDDDNGYIRWFVGDTPTYTIHAKALHPDGNIGWRRISKEP
MSIILNLGISNNWAYIDWQYIFFPVVMSIDYVRIYQPSNAISVTCDPSDYPTYDYIQS
HLNAFQNANLTTWEDAGYTFPKNILTGKCTSSKFKLSS"
gene complement(<860314..>860415)
/locus_tag="YPR159C-A"
/db_xref="GeneID:1466523"
mRNA complement(<860314..>860415)
/locus_tag="YPR159C-A"
/product="uncharacterized protein"
/transcript_id="NM_001184628.1"
/db_xref="GeneID:1466523"
CDS complement(860314..860415)
/locus_tag="YPR159C-A"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/note="hypothetical protein; SWAT-GFP and mCherry fusion
proteins localize to the cytosol; identified by
gene-trapping, microarray-based expression analysis, and
genome-wide homology searching"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878184.1"
/db_xref="GeneID:1466523"
/db_xref="SGD:S000028725"
/translation="MATLDFTTKPLALVIYMSVVLLLMVGVPLLFSS"
gene 860379..860449
/locus_tag="YNCP0023W"
/db_xref="GeneID:856288"
tRNA 860379..860449
/locus_tag="YNCP0023W"
/product="tRNA-Gly"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0030371 translation
repressor activity [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043555 regulation
of translation in response to stress [PMID:27609601]"
/note="Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:856288"
/db_xref="SGD:S000006589"
gene <861306..>864014
/gene="GPH1"
/locus_tag="YPR160W"
/db_xref="GeneID:856289"
mRNA <861306..>864014
/gene="GPH1"
/locus_tag="YPR160W"
/product="glycogen phosphorylase"
/transcript_id="NM_001184257.1"
/db_xref="GeneID:856289"
CDS 861306..864014
/gene="GPH1"
/locus_tag="YPR160W"
/EC_number="2.4.1.1"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:24146988|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008184 glycogen
phosphorylase activity [PMID:1613787]"
/experiment="EXISTENCE:mutant phenotype:GO:0005980
glycogen catabolic process [PMID:2657401]"
/experiment="EXISTENCE:mutant phenotype:GO:0008184
glycogen phosphorylase activity [PMID:2657401]"
/note="Glycogen phosphorylase required for the
mobilization of glycogen; non-essential; regulated by
cyclic AMP-mediated phosphorylation; phosphorylation by
Cdc28p may coordinately regulate carbohydrate metabolism
and the cell cycle; expression is regulated by
stress-response elements and by the HOG MAP kinase
pathway"
/codon_start=1
/product="glycogen phosphorylase"
/protein_id="NP_015486.1"
/db_xref="GeneID:856289"
/db_xref="SGD:S000006364"
/translation="MPPASTSTTNDMITEEPTSPHQIPRLTRRLTGFLPQEIKSIDTM
IPLKSRALWNKHQVKKFNKAEDFQDRFIDHVETTLARSLYNCDDMAAYEAASMSIRDN
LVIDWNKTQQKFTTRDPKRVYYLSLEFLMGRALDNALINMKIEDPEDPAASKGKPREM
IKGALDDLGFKLEDVLDQEPDAGLGNGGLGRLAACFVDSMATEGIPAWGYGLRYEYGI
FAQKIIDGYQVETPDYWLNSGNPWEIERNEVQIPVTFYGYVDRPEGGKTTLSASQWIG
GERVLAVAYDFPVPGFKTSNVNNLRLWQARPTTEFDFAKFNNGDYKNSVAQQQRAESI
TAVLYPNDNFAQGKELRLKQQYFWCAASLHDILRRFKKSKRPWTEFPDQVAIQLNDTH
PTLAIVELQRVLVDLEKLDWHEAWDIVTKTFAYTNHTVMQEALEKWPVGLFGHLLPRH
LEIIYDINWFFLQDVAKKFPKDVDLLSRISIIEENSPERQIRMAFLAIVGSHKVNGVA
ELHSELIKTTIFKDFVKFYGPSKFVNVTNGITPRRWLKQANPSLAKLISETLNDPTEE
YLLDMAKLTQLGKYVEDKEFLKKWNQVKLNNKIRLVDLIKKENDGVDIINREYLDDTL
FDMQVKRIHEYKRQQLNVFGIIYRYLAMKNMLKNGASIEEVAKKYPRKVSIFGGKSAP
GYYMAKLIIKLINCVADIVNNDESIEHLLKVVFVADYNVSKAEIIIPASDLSEHISTA
GTEASGTSNMKFVMNGGLIIGTVDGANVEITREIGEDNVFLFGNLSENVEELRYNHQY
HPQDLPSSLDSVLSYIESGQFSPENPNEFKPLVDSIKYHGDYYLVSDDFESYLATHEL
VDQEFHNQRSEWLKKSVLSVANVGFFSSDRCIEEYSDTIWNVEPVT"
gene complement(<864449..>866422)
/gene="SGV1"
/locus_tag="YPR161C"
/gene_synonym="BUR1"
/db_xref="GeneID:856290"
mRNA complement(<864449..>866422)
/gene="SGV1"
/locus_tag="YPR161C"
/gene_synonym="BUR1"
/product="cyclin-dependent serine/threonine protein kinase
SGV1"
/transcript_id="NM_001184258.1"
/db_xref="GeneID:856290"
CDS complement(864449..866422)
/gene="SGV1"
/locus_tag="YPR161C"
/gene_synonym="BUR1"
/EC_number="2.7.11.22"
/EC_number="2.7.11.23"
/experiment="EXISTENCE:direct assay:GO:0004693
cyclin-dependent protein serine/threonine kinase activity
[PMID:10982824]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14749387]"
/experiment="EXISTENCE:direct assay:GO:0006368
transcription elongation by RNA polymerase II
[PMID:10982824]"
/experiment="EXISTENCE:direct assay:GO:0008353 RNA
polymerase II CTD heptapeptide repeat kinase activity
[PMID:11390638]"
/experiment="EXISTENCE:genetic interaction:GO:0032968
positive regulation of transcription elongation by RNA
polymerase II [PMID:12972617]"
/experiment="EXISTENCE:mutant phenotype:GO:0006368
transcription elongation by RNA polymerase II
[PMID:10982824]"
/experiment="EXISTENCE:mutant phenotype:GO:0032968
positive regulation of transcription elongation by RNA
polymerase II [PMID:12972617]"
/experiment="EXISTENCE:physical interaction:GO:0000307
cyclin-dependent protein kinase holoenzyme complex
[PMID:10982824]"
/note="Cyclin (Bur2p)-dependent protein kinase; subunit of
the CDK-cyclin BUR kinase complex with Bur2p; regulates
Set2p-dependent H3K36 trimethylation; regulates
transcriptional elongation through the phosphorylation of
the carboxy-terminal domain (CTD) of Rpo21p; stimulates
recruitment of the PAF complex through Spt5p CTD
phosphorylation; recruits Spt6p to the CTD at the onset of
transcription; regulates telomere elongation; regulated by
Cak1p; similar to metazoan CDK9 proteins"
/codon_start=1
/product="cyclin-dependent serine/threonine protein kinase
SGV1"
/protein_id="NP_015487.1"
/db_xref="GeneID:856290"
/db_xref="SGD:S000006365"
/translation="MSDNGSPAVLPKTEFNKYKIGKVKSTPAIQRDAKTNLTYIKLRK
RSSEKVYGCTVFQNHYREDEKLGQGTFGEVYKGIHLETQRQVAMKKIIVSVEKDLFPI
TAQREITILKRLNHKNIIKLIEMVYDHSPDITNAASSNLHKSFYMILPYMVADLSGVL
HNPRINLEMCDIKNMMLQILEGLNYIHCAKFMHRDIKTANILIDHNGVLKLADFGLAR
LYYGCPPNLKYPGGAGSGAKYTSVVVTRWYRAPELVLGDKQYTTAVDIWGVGCVFAEF
FEKKPILQGKTDIDQGHVIFKLLGTPTEEDWAVARYLPGAELTTTNYKPTLRERFGKY
LSETGLDFLGQLLALDPYKRLTAMSAKHHPWFKEDPLPSEKITLPTEESHEADIKRYK
EEMHQSLSQRVPTAPRGHIVEKGESPVVKNLGAIPRGPKKDDASFLPPSKNVLAKPPP
SKIRELHQNPRPYHVNSGYAKTAIPPPAAPAGVNRYGPNNSSRNNRFSGNSTAPNNSR
NPVNRFHPETNVSSKYNKVPLPLGPQSRYQGNSNESRYKNSPNDSRYHNPRYVNKPET
NFNRQPQKYSRQESNAPINKNYNPSNGSRNMAGDHHQGSRPSHPQFPISPSQGQHQLT
SKPIEKKNGSFKDERAKPDESKEFQNSDIADLY"
gene complement(<866715..>868304)
/gene="ORC4"
/locus_tag="YPR162C"
/db_xref="GeneID:856291"
mRNA complement(<866715..>868304)
/gene="ORC4"
/locus_tag="YPR162C"
/product="origin recognition complex subunit 4"
/transcript_id="NM_001184259.1"
/db_xref="GeneID:856291"
CDS complement(866715..868304)
/gene="ORC4"
/locus_tag="YPR162C"
/experiment="EXISTENCE:direct assay:GO:0003688 DNA
replication origin binding [PMID:16824194]"
/experiment="EXISTENCE:direct assay:GO:0005656 nuclear
pre-replicative complex [PMID:9335335|PMID:16824194]"
/experiment="EXISTENCE:direct assay:GO:0005664 nuclear
origin of replication recognition complex [PMID:9372948]"
/experiment="EXISTENCE:direct assay:GO:0006267
pre-replicative complex assembly involved in nuclear cell
cycle DNA replication [PMID:16824194]"
/experiment="EXISTENCE:direct assay:GO:0030466 silent
mating-type cassette heterochromatin formation
[PMID:12897051]"
/experiment="EXISTENCE:direct assay:GO:0031261 DNA
replication preinitiation complex [PMID:9554851]"
/experiment="EXISTENCE:mutant phenotype:GO:0005664 nuclear
origin of replication recognition complex [PMID:9372948]"
/experiment="EXISTENCE:mutant phenotype:GO:0006270 DNA
replication initiation [PMID:16716188]"
/note="Subunit of the origin recognition complex (ORC);
ORC directs DNA replication by binding to replication
origins and is also involved in transcriptional silencing;
ORC4 has a paralog, RIF2, that arose from the whole genome
duplication"
/codon_start=1
/product="origin recognition complex subunit 4"
/protein_id="NP_015488.1"
/db_xref="GeneID:856291"
/db_xref="SGD:S000006366"
/translation="MTISEARLSPQVNLLPIKRHSNEEVEETAAILKKRTIDNEKCKD
SDPGFGSLQRRLLQQLYGTLPTDEKIIFTYLQDCQQEIDRIIKQSIIQKESHSVILVG
PRQSYKTYLLDYELSLLQQSYKEQFITIRLNGFIHSEQTAINGIATQLEQQLQKIHGS
EEKIDDTSLETISSGSLTEVFEKILLLLDSTTKTRNEDSGEVDRESITKITVVFIFDE
IDTFAGPVRQTLLYNLFDMVEHSRVPVCIFGCTTKLNILEYLEKRVKSRFSQRVIYMP
QIQNLDDMVDAVRNLLTVRSEISPWVSQWNETLEKELSDPRSNLNRHIRMNFETFRSL
PTLKNSIIPLVATSKNFGSLCTAIKSCSFLDIYNKNQLSNNLTGRLQSLSDLELAILI
SAARVALRAKDGSFNFNLAYAEYEKMIKAINSRIPTVAPTTNVGTGQSTFSIDNTIKL
WLKKDVKNVWENLVQLDFFTEKSAVGLRDNATAAFYASNYQFQGTMIPFDLRSYQMQI
ILQELRRIIPKSNMYYSWTQL"
gene complement(<868645..>869955)
/gene="TIF3"
/locus_tag="YPR163C"
/gene_synonym="RBL3; STM1"
/db_xref="GeneID:856292"
mRNA complement(<868645..>869955)
/gene="TIF3"
/locus_tag="YPR163C"
/gene_synonym="RBL3; STM1"
/product="Tif3p"
/transcript_id="NM_001184260.1"
/db_xref="GeneID:856292"
CDS complement(868645..869955)
/gene="TIF3"
/locus_tag="YPR163C"
/gene_synonym="RBL3; STM1"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:7543843]"
/experiment="EXISTENCE:direct assay:GO:0003727
single-stranded RNA binding [PMID:23236192]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0006413
translational initiation [PMID:7543843]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405]"
/experiment="EXISTENCE:direct assay:GO:0033592 RNA strand
annealing activity [PMID:7543843]"
/experiment="EXISTENCE:direct assay:GO:0034057 RNA
strand-exchange activity [PMID:9769100]"
/experiment="EXISTENCE:direct assay:GO:0043024 ribosomal
small subunit binding [PMID:23236192]"
/experiment="EXISTENCE:genetic interaction:GO:0006413
translational initiation [PMID:8404866]"
/experiment="EXISTENCE:genetic interaction:GO:0097010
eukaryotic translation initiation factor 4F complex
assembly [PMID:23184954]"
/experiment="EXISTENCE:mutant phenotype:GO:0001731
formation of translation preinitiation complex
[PMID:23236192]"
/experiment="EXISTENCE:mutant phenotype:GO:0002181
cytoplasmic translation [PMID:8404865]"
/experiment="EXISTENCE:mutant phenotype:GO:0006413
translational initiation [PMID:8404866]"
/experiment="EXISTENCE:mutant phenotype:GO:0097010
eukaryotic translation initiation factor 4F complex
assembly [PMID:23184954]"
/note="Translation initiation factor eIF-4B; RNA
recognition motif containing single-stranded RNA binding
protein that possesses RNA annealing and strand-exchange
activities; stimulates the ATPase and unwinding activities
of the RNA helicase eIF4A, in a synergistic manner with
eIF4G; promotes assembly of the translation initiation
factor complex eIF4F and the 48S preinitiation complex;
interacts with Rps20p at the head of the 40S ribosomal
subunit and alters the structure of the mRNA entry
channel"
/codon_start=1
/product="Tif3p"
/protein_id="NP_015489.1"
/db_xref="GeneID:856292"
/db_xref="SGD:S000006367"
/translation="MAPPKKTVKKMDLTSFLNDDTFGSSWAEEDVDLNKITIPIETAN
ANTIPLSELAHAKNNSNNTRSGGFGGSFGGRSRLDPALGGGSSDRREEYPVPDAPPYR
AVINNIPWDITPEGVQAWVEDGLVKPEAVEEVVLPKNLRDPTRLKGNAFVTLKERADL
VAVLKFNGTKLNERTVYVSVAAPRRGGGADVDWSSARGSNFQGDGREDAPDLDWGAAR
GSNFRGPRREREEVDIDWTAARGSNFQGSSRPPRREREEVDIDWSAARGSNFQGSSRP
PRREREEPDIDWSAARGSNFQSSSRPPRREREEPDIDWSAARGSNFQSSSRPPRRERE
KEEPALDWGAARGAQFGKPQQTKNTYKDRSLTNKKTTDEQPKIQKSVYDVLRTEDDDE
DEEAEKQNGDAKENKVDAAVEKLQDKTAQLTVEDGDNWEVVGKK"
gene <870703..>874926
/gene="MMS1"
/locus_tag="YPR164W"
/gene_synonym="RTT108; SLM6"
/db_xref="GeneID:856293"
mRNA <870703..>874926
/gene="MMS1"
/locus_tag="YPR164W"
/gene_synonym="RTT108; SLM6"
/product="Mms1p"
/transcript_id="NM_001184261.1"
/db_xref="GeneID:856293"
CDS 870703..874926
/gene="MMS1"
/locus_tag="YPR164W"
/gene_synonym="RTT108; SLM6"
/experiment="EXISTENCE:direct assay:GO:0035361 Cul8-RING
ubiquitin ligase complex [PMID:20139071]"
/experiment="EXISTENCE:mutant phenotype:GO:0000725
recombinational repair [PMID:18321796]"
/experiment="EXISTENCE:mutant phenotype:GO:0010526
transposable element silencing [PMID:11779788]"
/experiment="EXISTENCE:mutant phenotype:GO:0031297
replication fork processing [PMID:18704118]"
/experiment="EXISTENCE:mutant phenotype:GO:0070651
nonfunctional rRNA decay [PMID:19390089]"
/note="Subunit of E3 ubiquitin ligase complex involved in
replication repair; stabilizes protein components of the
replication fork such as the fork-pausing complex and
leading strand polymerase, preventing fork collapse and
promoting efficient recovery during replication stress;
regulates Ty1 transposition; involved with Rtt101p in
nonfunctional rRNA decay"
/codon_start=1
/product="Mms1p"
/protein_id="NP_015490.1"
/db_xref="GeneID:856293"
/db_xref="SGD:S000006368"
/translation="MLGLRTHGLDRYEHYIRRPSDFGKLELQDWLNHKSFRVSPNLLI
DSSTTREWNEPELFYQNTEDETWVRPCVGPKLEPSMMMLRYHDSNIGQMPQFCYPISS
PINFKPVLKYILQERSELSDGFPQKYNTLIGSLFDIDKNPETLDDSDIEALDDIEMSS
DSGNVKEPKIELQALEEIQQKHFSLIVSNNGIFQTGSTSITYIQSGISGSIAIKPNNV
AILILLTQPSGHLLSILPLDDGKETYLLQYWNLGQKGQWNIIKHQNEKQFVLIHKELG
ICKFFEFHLPFTFQLVNNLTLTDSVIMNGSFFPTNYTDLDPYFIIFITAIRYERIVYF
VIEWNNNEIKKKEVYQLTVFDGEKTNMTIPIGLNACLVETPLKFSLVSANQIMSGETE
FHSFQLKALKGIKSFFPAPLLLLKLQELHPHTFKKFQYCTIISSSTGNICFCVTERST
IVNGNLKFYELTRFKGLKSISPLPSNPINLDSRSSSYVLVVISFSRTLELTLSLEDLR
CLDKKDVIKPLKNITFKHTIDSSTEENSQILAFTSSKFYNTHTGSNINDTRNSQVWLT
SPNAITQPCIDYKLRKTHQLIHLKQFQIFRHLRIWKCKNLDIALLQRLGINQSNTESS
LIFATDAVSNNRIFLLDLTMTTTIDNDDPVQGLINIEDLLCDTENETILLNFTKNNLI
QVTRDTIYIDPIGGDKELRKISPGWEFENVTYNDGILIVWNAGLGCVSYIENIDAVDE
SGALVSNLSSSKGMSKFFKQLGTVTSVNFQIKESTDDPTKYDIWILLPDCVIRTPFSD
WISDSLDFSDVYILSVQQALINGPYFCSLDYESYFEVHTLQNNCFKKGSRCTSRVNFQ
GKDIKFRSFGVNQCLAFSAFEIFVINLTPIHDSRELDFYKLKLPHLGNNNSILEVCPD
IENNQLFILYSDGLRILELSYLTSNNGNFLLKSTRSKNKKFLYLDKINRMLVLNQDLR
EWECIRLSDGKAVGLDSQLLKDDSEEILEIKELPIATEDNPLEKKTVLLISFTSSLKL
VLLTAAKNKISNQIIDSYKLDNSRLLNHLVITPRGEIFFLDYKVMGTDNEMSFNKLKV
TKHCIDQEERNNTTLRLTLETRFTFKSWSTVKTFTVVGDNIIATTNMGEKLYLIKDFS
SSSDESRRVYPLEMYPDSKVQKIIPLNECCFVVAAYCGNRNDLDSRLIFYSLPTIKVG
LNNETGSLPDEYGNGRVDDIFEVDFPEGFQFGTMALYDVLHGERHVNRYSEGIRSEND
EAEVALRQRRNLLLFWRNHSSTPKPSLRRAATIVYEDHVSSRYFEDISSILGSTAMRT
KRLSPYNAVALDKPIQDISYDPAVQTLYVLMADQTIHKFGKDRLPCQDEYEPRWNSGY
LVSRRSIVKSDLICEVGLWNLSDNCKNTV"
gene <875368..>875997
/gene="RHO1"
/locus_tag="YPR165W"
/db_xref="GeneID:856294"
mRNA <875368..>875997
/gene="RHO1"
/locus_tag="YPR165W"
/product="Rho family GTPase RHO1"
/transcript_id="NM_001184262.1"
/db_xref="GeneID:856294"
CDS 875368..875997
/gene="RHO1"
/locus_tag="YPR165W"
/EC_number="3.6.5.2"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:8195291]"
/experiment="EXISTENCE:direct assay:GO:0000148
1,3-beta-D-glucan synthase complex [PMID:8602515]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:11598008]"
/experiment="EXISTENCE:direct assay:GO:0003924 GTPase
activity [PMID:7962098|PMID:11591390]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:16407407]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:15596542]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:1918143]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836]"
/experiment="EXISTENCE:direct assay:GO:0005933 cellular
bud [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:8195291]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:8195291]"
/experiment="EXISTENCE:direct assay:GO:0007264 small
GTPase-mediated signal transduction [PMID:8621575]"
/experiment="EXISTENCE:direct assay:GO:0008047 enzyme
activator activity [PMID:37703377|PMID:36949198]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:12660244]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0090334 regulation
of cell wall (1->3)-beta-D-glucan biosynthetic process
[PMID:36949198|PMID:8602515|PMID:8662910]"
/experiment="EXISTENCE:genetic interaction:GO:0007117
budding cell bud growth [PMID:8195291]"
/experiment="EXISTENCE:genetic interaction:GO:0007264
small GTPase-mediated signal transduction [PMID:8846785]"
/experiment="EXISTENCE:genetic interaction:GO:0030036
actin cytoskeleton organization [PMID:8947028]"
/experiment="EXISTENCE:genetic interaction:GO:1903501
positive regulation of mitotic actomyosin contractile ring
assembly [PMID:12419188]"
/experiment="EXISTENCE:mutant phenotype:GO:0007117 budding
cell bud growth [PMID:8195291]"
/experiment="EXISTENCE:mutant phenotype:GO:0008361
regulation of cell size [PMID:17302939]"
/experiment="EXISTENCE:mutant phenotype:GO:0030036 actin
cytoskeleton organization [PMID:15596542]"
/experiment="EXISTENCE:mutant phenotype:GO:0032186
cellular bud neck septin ring organization
[PMID:26179915]"
/experiment="EXISTENCE:mutant phenotype:GO:0032880
regulation of protein localization [PMID:10893184]"
/experiment="EXISTENCE:mutant phenotype:GO:0032889
regulation of vacuole fusion, non-autophagic
[PMID:11598008]"
/experiment="EXISTENCE:mutant phenotype:GO:0045807
positive regulation of endocytosis [PMID:14593073]"
/experiment="EXISTENCE:mutant phenotype:GO:0060178
regulation of exocyst localization [PMID:11283608]"
/experiment="EXISTENCE:mutant phenotype:GO:0060237
regulation of fungal-type cell wall organization
[PMID:8621575]"
/experiment="EXISTENCE:mutant phenotype:GO:0090334
regulation of cell wall (1->3)-beta-D-glucan biosynthetic
process [PMID:8602515|PMID:8602514|PMID:8662910]"
/experiment="EXISTENCE:mutant phenotype:GO:1903395
regulation of secondary cell septum biogenesis
[PMID:23878277]"
/experiment="EXISTENCE:mutant phenotype:GO:1903501
positive regulation of mitotic actomyosin contractile ring
assembly [PMID:12419188]"
/experiment="EXISTENCE:physical interaction:GO:0030036
actin cytoskeleton organization [PMID:8947028]"
/experiment="EXISTENCE:physical interaction:GO:0031681
G-protein beta-subunit binding [PMID:12660244]"
/note="GTP-binding protein of the rho subfamily of
Ras-like small GTPases; involved in establishment of cell
polarity; regulates protein kinase C (Pkc1p); positive
regulatory subunit of the 1,3-beta-D-glucan synthase
complex, activating 1,3-beta-glucan synthase (Fks1p and
Gsc2p), an enzyme involved in cell wall biosynthesis"
/codon_start=1
/product="Rho family GTPase RHO1"
/protein_id="NP_015491.1"
/db_xref="GeneID:856294"
/db_xref="SGD:S000006369"
/translation="MSQQVGNSIRRKLVIVGDGACGKTCLLIVFSKGQFPEVYVPTVF
ENYVADVEVDGRRVELALWDTAGQEDYDRLRPLSYPDSNVVLICFSIDLPDSLENVQE
KWIAEVLHFCQGVPIILVGCKVDLRNDPQTIEQLRQEGQQPVTSQEGQSVADQIGATG
YYECSAKTGYGVREVFEAATRASLMGKSKTNGKAKKNTTEKKKKKCVLL"
gene complement(<876282..>876629)
/gene="MRP2"
/locus_tag="YPR166C"
/gene_synonym="uS14m"
/db_xref="GeneID:856295"
mRNA complement(<876282..>876629)
/gene="MRP2"
/locus_tag="YPR166C"
/gene_synonym="uS14m"
/product="mitochondrial 37S ribosomal protein uS14m MRP2"
/transcript_id="NM_001184263.1"
/db_xref="GeneID:856295"
CDS complement(876282..876629)
/gene="MRP2"
/locus_tag="YPR166C"
/gene_synonym="uS14m"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:11278769]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:11278769]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005763
mitochondrial small ribosomal subunit [PMID:11278769]"
/note="Mitochondrial ribosomal protein of the small
subunit"
/codon_start=1
/product="mitochondrial 37S ribosomal protein uS14m MRP2"
/protein_id="NP_015492.1"
/db_xref="GeneID:856295"
/db_xref="SGD:S000006370"
/translation="MGNFRFPIKTKLPPGFINARILRDNFKRQQFKENEILVKSLKFI
ARNMNLPTKLRLEAQLKLNALPNYMRSTQIKNRCVDSGHARFVLSDFRLCRYQFRENA
LKGNLPGVKKGIW"
gene complement(<876847..>877632)
/gene="MET16"
/locus_tag="YPR167C"
/db_xref="GeneID:856296"
mRNA complement(<876847..>877632)
/gene="MET16"
/locus_tag="YPR167C"
/product="phosphoadenylyl-sulfate reductase (thioredoxin)"
/transcript_id="NM_001184264.1"
/db_xref="GeneID:856296"
CDS complement(876847..877632)
/gene="MET16"
/locus_tag="YPR167C"
/EC_number="1.8.4.8"
/experiment="EXISTENCE:curator inference:GO:0005737
cytoplasm [PMID:3060034]"
/experiment="EXISTENCE:direct assay:GO:0004604
phosphoadenylyl-sulfate reductase (thioredoxin) activity
[PMID:3060034]"
/experiment="EXISTENCE:direct assay:GO:0019379 sulfate
assimilation, phosphoadenylyl sulfate reduction by
phosphoadenylyl-sulfate reductase (thioredoxin)
[PMID:3060034]"
/experiment="EXISTENCE:mutant phenotype:GO:0004604
phosphoadenylyl-sulfate reductase (thioredoxin) activity
[PMID:2203779]"
/experiment="EXISTENCE:mutant phenotype:GO:0019379 sulfate
assimilation, phosphoadenylyl sulfate reduction by
phosphoadenylyl-sulfate reductase (thioredoxin)
[PMID:197388]"
/note="3'-phosphoadenylsulfate reductase; reduces
3'-phosphoadenylyl sulfate to adenosine-3',5'-bisphosphate
and free sulfite using reduced thioredoxin as cosubstrate,
involved in sulfate assimilation and methionine
metabolism"
/codon_start=1
/product="phosphoadenylyl-sulfate reductase (thioredoxin)"
/protein_id="NP_015493.1"
/db_xref="GeneID:856296"
/db_xref="SGD:S000006371"
/translation="MKTYHLNNDIIVTQEQLDHWNEQLIKLETPQEIIAWSIVTFPHL
FQTTAFGLTGLVTIDMLSKLSEKYYMPELLFIDTLHHFPQTLTLKNEIEKKYYQPKNQ
TIHVYKPDGCESEADFASKYGDFLWEKDDDKYDYLAKVEPAHRAYKELHISAVFTGRR
KSQGSARSQLSIIEIDELNGILKINPLINWTFEQVKQYIDANNVPYNELLDLGYRSIG
DYHSTQPVKEGEDERAGRWKGKAKTECGIHEASRFAQFLKQDA"
gene <878076..>878549
/gene="NUT2"
/locus_tag="YPR168W"
/gene_synonym="MED10"
/db_xref="GeneID:856297"
mRNA <878076..>878549
/gene="NUT2"
/locus_tag="YPR168W"
/gene_synonym="MED10"
/product="mediator complex subunit NUT2"
/transcript_id="NM_001184265.1"
/db_xref="GeneID:856297"
CDS 878076..878549
/gene="NUT2"
/locus_tag="YPR168W"
/gene_synonym="MED10"
/experiment="EXISTENCE:direct assay:GO:0070847 core
mediator complex [PMID:9891034]"
/experiment="EXISTENCE:genetic interaction:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:9671481]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:16452140]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:9891034]"
/experiment="EXISTENCE:mutant phenotype:GO:0051123 RNA
polymerase II preinitiation complex assembly
[PMID:27688401]"
/note="Subunit of the RNA polymerase II mediator complex;
conserved from yeast to human; associates with core
polymerase subunits to form the RNA polymerase II
holoenzyme; required for transcriptional activation and
has a role in basal transcription; protein abundance
increases in response to DNA replication stress"
/codon_start=1
/product="mediator complex subunit NUT2"
/protein_id="NP_015494.1"
/db_xref="GeneID:856297"
/db_xref="SGD:S000006372"
/translation="MNGNSTNNEQLQQELATTQDQVASIIESFVELGVSIYDFPGTPE
ATKGMITNLQRNVDRLYKLNVRSNDPQSSLSKVDIPLEVVQYIEDGRNPDIYTREFVE
AIRRSNQYQRGKMHGLKQLRDSLADKIVDEFPELKEPVEDIIKRTSPIDNVSNTH"
gene <878690..>880168
/gene="JIP5"
/locus_tag="YPR169W"
/db_xref="GeneID:856298"
mRNA <878690..>880168
/gene="JIP5"
/locus_tag="YPR169W"
/product="Jip5p"
/transcript_id="NM_001184266.1"
/db_xref="GeneID:856298"
CDS 878690..880168
/gene="JIP5"
/locus_tag="YPR169W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14690591|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:14562095|PMID:14690591]"
/experiment="EXISTENCE:mutant phenotype:GO:0042273
ribosomal large subunit biogenesis [PMID:19806183]"
/note="Protein required for biogenesis of the large
ribosomal subunit; required for biogenesis of the large
ribosomal subunit; interacts with proteins involved in RNA
processing, ribosome biogenesis, ubiquitination and
demethylation; similar to WDR55, a human WD repeat
protein; essential gene"
/codon_start=1
/product="Jip5p"
/protein_id="NP_015495.2"
/db_xref="GeneID:856298"
/db_xref="SGD:S000006373"
/translation="MAKSKKKTDVVDSTNLPILELLSLKAPIFQSLLHPELPIIITGF
GTGHIVCHRYDPAKLQSHLDRRRRIDTATTGKDAKKGVCPWIRLDIDLETGDLKFVDI
EEQQQQKQTGKDEDLGVKTLWKTKRHKGSVRAMCFDSKGDNIFSVGSDNVLKKANTMT
GKVVKKVNLSSLFNSEEKKNDKFTKLCASQTHPFILIGDESGNIHVINSENLALSNSI
RSIHFGDSINDIFHFDKRSAYKFISLGQTTLAYFDVRDKDAKPNVAGNEDGKILISDD
QEDEVLCGCFVDPEVADTLLCGMGEGIVTVWKPNKNDLEDQMSRIKISKDESIDCIVP
TLQDDNCVWCGCSNGNIYKVNAKLGKVVEIRNHSELDEVSFVDLDFEYRVVSGGLENI
KIWELSSDDVEENASVESDSDEPLSHSDEDLSDDTSSDDETTLVGLSKEELLDELDKD
LKEDHQEEKESNSKSVKKRKIMKENNKKKDLYEHGIKKFDDL"
gene complement(880296..880369)
/locus_tag="YNCP0024C"
/db_xref="GeneID:856299"
tRNA complement(880296..880369)
/locus_tag="YNCP0024C"
/product="tRNA-Ile"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Isoleucine tRNA (tRNA-Ile), predicted by
tRNAscan-SE analysis"
/db_xref="GeneID:856299"
/db_xref="SGD:S000006612"
repeat_region 880386..880726
/note="Ty3 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007218"
rep_origin 880861..881108
/note="ARS1630; Autonomously Replicating Sequence"
/db_xref="SGD:S000118425"
repeat_region complement(881487..881830)
/note="Ty4 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007207"
gene <883239..>883595
/locus_tag="YPR170W-B"
/db_xref="GeneID:5848745"
mRNA join(<883239..883387,883487..>883595)
/locus_tag="YPR170W-B"
/product="uncharacterized protein"
/transcript_id="NM_001184525.1"
/db_xref="GeneID:5848745"
CDS join(883239..883387,883487..883595)
/locus_tag="YPR170W-B"
/note="Putative, non-essential component vacuolar ATPase;
conserved in fungi; SWAT-GFP fusion protein localizes to
vacuole membrane; partially overlaps the dubious genes
YPR169W-A, YPR170W-A and YRP170C"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001106949.1"
/db_xref="GeneID:5848745"
/db_xref="SGD:S000028515"
/translation="MRPVVSTGKAWCCTVLSAFGVVILSVIAHLFNTNHESFVGSIND
PEDGPAVAHTVYLAALVYLVFFVFCGFQVYLARRKPSIELR"
gene <883828..>885558
/gene="BSP1"
/locus_tag="YPR171W"
/db_xref="GeneID:856301"
mRNA <883828..>885558
/gene="BSP1"
/locus_tag="YPR171W"
/product="Bsp1p"
/transcript_id="NM_001184268.1"
/db_xref="GeneID:856301"
CDS 883828..885558
/gene="BSP1"
/locus_tag="YPR171W"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:12606027]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:12606027]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:12606027]"
/experiment="EXISTENCE:direct assay:GO:0030479 actin
cortical patch [PMID:38088874|PMID:12606027]"
/experiment="EXISTENCE:direct assay:GO:0030674
protein-macromolecule adaptor activity [PMID:12606027]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807]"
/experiment="EXISTENCE:direct assay:GO:0051015 actin
filament binding [PMID:38088874]"
/experiment="EXISTENCE:direct assay:GO:0110085 mitotic
actomyosin contractile ring [PMID:38088874]"
/experiment="EXISTENCE:mutant phenotype:GO:0044379 protein
localization to actin cortical patch [PMID:38088874]"
/experiment="EXISTENCE:mutant phenotype:GO:0051015 actin
filament binding [PMID:38088874]"
/experiment="EXISTENCE:mutant phenotype:GO:0051666 actin
cortical patch localization [PMID:12606027]"
/experiment="EXISTENCE:mutant phenotype:GO:1904498 protein
localization to mitotic actomyosin contractile ring
[PMID:38088874]"
/note="Actin filament binding protein; regulates actin
filament capping by recruiting capping protein to
endocytic sites and to the cytokinetic actin ring;
interacts with the capping protein complex; adapter that
links synaptojanins, Inp52p and Inp53p, to the cortical
actin cytoskeleton; contains two WH2-like actin binding
domains and a C-terminal capping protein interaction (CPI)
motif"
/codon_start=1
/product="Bsp1p"
/protein_id="NP_015497.1"
/db_xref="GeneID:856301"
/db_xref="SGD:S000006375"
/translation="MTKYERDPELVNFLSKVEDLNSKRYSNIPSSKPAGEALSPVRSH
NSGEYRRADMMTGKNVEGCDNLAYRSAYNYEMTFSPKKTHYSLSELNLERITPRPDLE
GSASQKEKKFLISEEDYLLLQKLKASQTYNDSNADKNLPSFEKGPRMPSRGRPRPREK
EIITIQYDFELPGRADIPSSSSSSSPPPLPTRRDHIKITDGNEEKPLLPTRPNKAEVT
ESPSSRSIKPDAVVPERVKPAPPVSRSTKPASFLSSLEDNKLTKAKSYNSEMETPKTT
VKSSHIDYLDSIQLKPTTLSPTMKNKPKPTPPSPPAKRIPRSESFIKSMLNSNLTTTS
KPSLPEKPQKLRNANLAAHKTKPSIPPKKVELNIVLPELRPVETSPTKQNFENSIDLP
KLRSSNRNIKKEEEDSIPEAIKGIQNLKKTKQQKPAIPQKKSFLTNNSKNTTLKNGDD
INKLNDEIEALSLRNNLKKRPPTAPQRKISLPEALRKVELMKKSKTEPVLESSNELSI
NAKLDAIIASRNLRASNTLPELSGVNTNIATSDKYTTSRDETVKETKPLVHPNKNRTR
GPRRKLPTRV"
gene <885796..>886398
/locus_tag="YPR172W"
/db_xref="GeneID:856302"
mRNA <885796..>886398
/locus_tag="YPR172W"
/product="pyridoxal 5'-phosphate synthase"
/transcript_id="NM_001184269.1"
/db_xref="GeneID:856302"
CDS 885796..886398
/locus_tag="YPR172W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276|PMID:14562095]"
/note="hypothetical protein; predicted to encode a
pyridoxal 5'-phosphate synthase based on sequence
similarity but purified protein does not possess this
activity, nor does it bind flavin mononucleotide (FMN);
transcriptionally activated by Yrm1p along with genes
involved in multidrug resistance; YPR172W has a paralog,
YLR456W, that arose from the whole genome duplication"
/codon_start=1
/product="pyridoxal 5'-phosphate synthase"
/protein_id="NP_015498.1"
/db_xref="GeneID:856302"
/db_xref="SGD:S000006376"
/translation="MAWTSTLPAHLLNLIKNSKYVHVATCSKDCIPSVALMNYIYVPG
EKLFGQTDNKNDYIIFVSPQDTQKFYNIKENPKVALLFHDWIIANNLSVGKESISGTP
TPTSIPHEEQRQSELLNLLQELNQAELNQMSASIGGETEIVNPESEESKYYKDLILKA
NPDAKAFIFEKNTAVVKVRIDNARVSNNENRTMFLSKGKS"
gene complement(<886524..>887837)
/gene="VPS4"
/locus_tag="YPR173C"
/gene_synonym="CSC1; DID6; END13; GRD13; VPL4; VPT10"
/db_xref="GeneID:856303"
mRNA complement(<886524..>887837)
/gene="VPS4"
/locus_tag="YPR173C"
/gene_synonym="CSC1; DID6; END13; GRD13; VPL4; VPT10"
/product="AAA family ATPase VPS4"
/transcript_id="NM_001184270.1"
/db_xref="GeneID:856303"
CDS complement(886524..887837)
/gene="VPS4"
/locus_tag="YPR173C"
/gene_synonym="CSC1; DID6; END13; GRD13; VPL4; VPT10"
/experiment="EXISTENCE:direct assay:GO:0005524 ATP binding
[PMID:9606181]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:9606181]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:16601096]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity
[PMID:16505166|PMID:9155008|PMID:9606181]"
/experiment="EXISTENCE:direct assay:GO:0061709
reticulophagy [PMID:31802527]"
/experiment="EXISTENCE:genetic interaction:GO:0016125
sterol metabolic process [PMID:16096648]"
/experiment="EXISTENCE:mutant phenotype:GO:0005524 ATP
binding [PMID:9606181]"
/experiment="EXISTENCE:mutant phenotype:GO:0016125 sterol
metabolic process [PMID:16096648]"
/experiment="EXISTENCE:mutant phenotype:GO:0016236
macroautophagy [PMID:17428789]"
/experiment="EXISTENCE:mutant phenotype:GO:0016887 ATP
hydrolysis activity [PMID:9155008]"
/experiment="EXISTENCE:mutant phenotype:GO:0032511 late
endosome to vacuole transport via multivesicular body
sorting pathway [PMID:16505166]"
/experiment="EXISTENCE:mutant phenotype:GO:0045053 protein
retention in Golgi apparatus [PMID:8649377]"
/experiment="EXISTENCE:mutant phenotype:GO:0045324 late
endosome to vacuole transport [PMID:9606181|PMID:9211789]"
/experiment="EXISTENCE:mutant phenotype:GO:0070676
intralumenal vesicle formation [PMID:19234443]"
/note="AAA-ATPase involved in multivesicular body (MVB)
protein sorting; ATP-bound Vps4p localizes to endosomes
and catalyzes ESCRT-III disassembly and membrane release;
ATPase activity is activated by Vta1p; regulates cellular
sterol metabolism"
/codon_start=1
/product="AAA family ATPase VPS4"
/protein_id="NP_015499.1"
/db_xref="GeneID:856303"
/db_xref="SGD:S000006377"
/translation="MSTGDFLTKGIELVQKAIDLDTATQYEEAYTAYYNGLDYLMLAL
KYEKNPKSKDLIRAKFTEYLNRAEQLKKHLESEEANAAKKSPSAGSGSNGGNKKISQE
EGEDNGGEDNKKLRGALSSAILSEKPNVKWEDVAGLEGAKEALKEAVILPVKFPHLFK
GNRKPTSGILLYGPPGTGKSYLAKAVATEANSTFFSVSSSDLVSKWMGESEKLVKQLF
AMARENKPSIIFIDEVDALTGTRGEGESEASRRIKTELLVQMNGVGNDSQGVLVLGAT
NIPWQLDSAIRRRFERRIYIPLPDLAARTTMFEINVGDTPCVLTKEDYRTLGAMTEGY
SGSDIAVVVKDALMQPIRKIQSATHFKDVSTEDDETRKLTPCSPGDDGAIEMSWTDIE
ADELKEPDLTIKDFLKAIKSTRPTVNEDDLLKQEQFTRDFGQEGN"
gene complement(<888043..>888708)
/gene="CSA1"
/locus_tag="YPR174C"
/db_xref="GeneID:856304"
mRNA complement(<888043..>888708)
/gene="CSA1"
/locus_tag="YPR174C"
/product="Csa1p"
/transcript_id="NM_001184271.1"
/db_xref="GeneID:856304"
CDS complement(888043..888708)
/gene="CSA1"
/locus_tag="YPR174C"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:15282802]"
/experiment="EXISTENCE:direct assay:GO:0005816 spindle
pole body [PMID:15282802]"
/experiment="EXISTENCE:direct assay:GO:0034399 nuclear
periphery [PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:1902365 positive
regulation of protein localization to spindle pole body
[PMID:32553169]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to the nuclear periphery;
potential Cdc28p substrate; binds phosphatidylinositols
and phosphatidylethanolamine in a large-scale study;
relative distribution to foci at the nuclear periphery
increases upon DNA replication stress; YPR174C has a
paralog, NBP1, that arose from the whole genome
duplication"
/codon_start=1
/product="Csa1p"
/protein_id="NP_015500.1"
/db_xref="GeneID:856304"
/db_xref="SGD:S000006378"
/translation="MGIQEKTLGIRKERKLVVVPRERNHVRHASQRTRSKNYKNISKK
RAQQHAFGFNIAKTLAKIQAFVWGSPADEEEESVVPLSKNSQDCVPLQWQAKFAQLRQ
QLHSTQKELQFVKEKCHLLQSVLDDANIDQRYLESRRDMKNIERDNLKPTENLPPSPV
RAVNPLVTSSPIHMSPLQSRQRPVSSLQPPKGPNFYAKYPKLPQTNILRESPTEDSVP
HAE"
gene <888974..>891043
/gene="DPB2"
/locus_tag="YPR175W"
/db_xref="GeneID:856305"
mRNA <888974..>891043
/gene="DPB2"
/locus_tag="YPR175W"
/product="DNA polymerase epsilon noncatalytic subunit"
/transcript_id="NM_001184272.1"
/db_xref="GeneID:856305"
CDS 888974..891043
/gene="DPB2"
/locus_tag="YPR175W"
/experiment="EXISTENCE:direct assay:GO:0003690
double-stranded DNA binding [PMID:14622139|PMID:16916794]"
/experiment="EXISTENCE:direct assay:GO:0003697
single-stranded DNA binding [PMID:14622139]"
/experiment="EXISTENCE:direct assay:GO:0003887
DNA-directed DNA polymerase activity [PMID:12882968]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:15282802]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:15282802]"
/experiment="EXISTENCE:direct assay:GO:0006261
DNA-templated DNA replication
[PMID:16369485|PMID:14622139|PMID:12882968]"
/experiment="EXISTENCE:direct assay:GO:0008622 epsilon DNA
polymerase complex
[PMID:2406268|PMID:14673157|PMID:12571237]"
/experiment="EXISTENCE:direct assay:GO:0030337 DNA
polymerase processivity factor activity [PMID:16369485]"
/experiment="EXISTENCE:direct assay:GO:0042276 error-prone
translesion synthesis [PMID:19776424]"
/experiment="EXISTENCE:mutant phenotype:GO:0042276
error-prone translesion synthesis [PMID:19776424]"
/experiment="EXISTENCE:mutant phenotype:GO:0045005
DNA-templated DNA replication maintenance of fidelity
[PMID:18245343|PMID:19463834]"
/note="Second largest subunit of DNA polymerase II (DNA
polymerase epsilon); required for maintenance of fidelity
of chromosomal replication; essential motif in C-terminus
is required for formation of the four-subunit Pol epsilon;
expression peaks at the G1/S phase boundary; Cdc28p
substrate"
/codon_start=1
/product="DNA polymerase epsilon noncatalytic subunit"
/protein_id="NP_015501.2"
/db_xref="GeneID:856305"
/db_xref="SGD:S000006379"
/translation="MFGSGNVLPVKIQPPLLRPLAYRVLSRKYGLSIKSDGLSALAEF
VGTNIGANWRQGPATIKFLEQFAAVWKQQERGLFIDQSGVKEVIQEMKEREKVEWSHE
HPIQHEENILGRTDDDENNSDDEMPIAADSSLQNVSLSSPMRQPTERDEYKQPFKPES
SKALDWRDYFKVINASQQQRFSYNPHKMQFIFVPNKKQNGLGGIAGFLPDIEDKVQMF
LTRYYLTNDRVMRNENFQNSDMFNPLSSMVSLQNELSNTNRQQQSSSMSITPIKNLLG
RDAQNFLLLGLLNKNFKGNWSLEDPSGSVEIDISQTIPTQGHYYVPGCMVLVEGIYYS
VGNKFHVTSMTLPPGERREITLETIGNLDLLGIHGISNNNFIARLDKDLKIRLHLLEK
ELTDHKFVILGANLFLDDLKIMTALSKILQKLNDDPPTLLIWQGSFTSVPVFASMSSR
NISSSTQFKNNFDALATLLSRFDNLTENTTMIFIPGPNDLWGSMVSLGASGTLPQDPI
PSAFTKKINKVCKNVVWSSNPTRIAYLSQEIVIFRDDLSGRFKRHRLEFPFNESEDVY
TENDNMMSKDTDIVPIDELVKEPDQLPQKVQETRKLVKTILDQGHLSPFLDSLRPISW
DLDHTLTLCPIPSTMVLCDTTSAQFDLTYNGCKVINPGSFIHNRRARYMEYVPSSKKT
IQEEIYI"
gene complement(<891101..>892078)
/gene="BET2"
/locus_tag="YPR176C"
/db_xref="GeneID:856306"
mRNA complement(<891101..>892078)
/gene="BET2"
/locus_tag="YPR176C"
/product="Rab geranylgeranyltransferase BET2"
/transcript_id="NM_001184273.1"
/db_xref="GeneID:856306"
CDS complement(891101..892078)
/gene="BET2"
/locus_tag="YPR176C"
/EC_number="2.5.1.60"
/experiment="EXISTENCE:direct assay:GO:0004663 Rab
geranylgeranyltransferase activity [PMID:8756702]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005968 Rab-protein
geranylgeranyltransferase complex
[PMID:8183917|PMID:8756702|PMID:8583924]"
/experiment="EXISTENCE:direct assay:GO:0018344 protein
geranylgeranylation [PMID:8756702]"
/experiment="EXISTENCE:direct assay:GO:0170069
geranylgeranyltransferase-III complex [PMID:40049413]"
/experiment="EXISTENCE:mutant phenotype:GO:0006612 protein
targeting to membrane [PMID:1903184]"
/experiment="EXISTENCE:mutant phenotype:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:8232541|PMID:3312234]"
/experiment="EXISTENCE:physical interaction:GO:0005968
Rab-protein geranylgeranyltransferase complex
[PMID:8232542]"
/note="Beta subunit of both type II and type III
geranylgeranyltransferase complexes; provides a prenylated
membrane attachment moiety for Rab-like proteins Ypt1p and
Sec4p as part of GGTase-II; functions with alpha subunit
Ecm9p (GGTase-III) in the transfer of a geranylgeranyl
group to mono-farnsylated Ykt6p, regulating membrane
localization and function in cell wall integrity and
autophagy; required for vesicular transport between the ER
and Golgi; human homolog RabGGTB, can complement yeast
BET2 mutants"
/codon_start=1
/product="Rab geranylgeranyltransferase BET2"
/protein_id="NP_015502.1"
/db_xref="GeneID:856306"
/db_xref="SGD:S000006380"
/translation="MSGSLTLLKEKHIRYIESLDTKKHNFEYWLTEHLRLNGIYWGLT
ALCVLDSPETFVKEEVISFVLSCWDDKYGAFAPFPRHDAHLLTTLSAVQILATYDALD
VLGKDRKVRLISFIRGNQLEDGSFQGDRFGEVDTRFVYTALSALSILGELTSEVVDPA
VDFVLKCYNFDGGFGLCPNAESHAAQAFTCLGALAIANKLDMLSDDQLEEIGWWLCER
QLPEGGLNGRPSKLPDVCYSWWVLSSLAIIGRLDWINYEKLTEFILKCQDEKKGGISD
RPENEVDVFHTVFGVAGLSLMGYDNLVPIDPIYCMPKSVTSKFKKYPYK"
gene <892332..>893729
/gene="PRP4"
/locus_tag="YPR178W"
/gene_synonym="RNA4"
/db_xref="GeneID:856307"
mRNA <892332..>893729
/gene="PRP4"
/locus_tag="YPR178W"
/gene_synonym="RNA4"
/product="U4/U6-U5 snRNP complex subunit PRP4"
/transcript_id="NM_001184275.1"
/db_xref="GeneID:856307"
CDS 892332..893729
/gene="PRP4"
/locus_tag="YPR178W"
/gene_synonym="RNA4"
/experiment="EXISTENCE:direct assay:GO:0046540 U4/U6 x U5
tri-snRNP complex [PMID:10449419|PMID:10377396]"
/experiment="EXISTENCE:mutant phenotype:GO:0000393
spliceosomal conformational changes to generate catalytic
conformation [PMID:28864812]"
/experiment="EXISTENCE:mutant phenotype:GO:0034247 snoRNA
splicing [PMID:8202378]"
/note="Splicing factor; component of the U4/U6-U5 snRNP
complex"
/codon_start=1
/product="U4/U6-U5 snRNP complex subunit PRP4"
/protein_id="NP_015504.1"
/db_xref="GeneID:856307"
/db_xref="SGD:S000006382"
/translation="MSKYIALENLPVDLQHKGATQNESTADILKQLPHERLQAVLEKI
PEEDLEVRRLLSILKKPEVVENEDVQQRRIRLAEILMVDEIDLENINNMENINGEEVD
EEDDEDFFTPATSELIFARRFLINYSLERSRKRLQKEMERHQKFNTRQELLSRRTELQ
RMANLELAGSQLVSTKPISAVSLSTDDMVVATGSWAGDLQVLNSQTLQPLTQKLDSHV
GKIGAIDWHPDSNNQMISCAEDGLIKNFQYSNEEGGLRLLGDLVGHERRISDVKYHPS
GKFIGSASHDMTWRLWDASTHQELLLQEGHDKGVFSLSFQCDGSLVCSGGMDSLSMLW
DIRSGSKVMTLAGHSKPIYTVAWSPNGYQVATGGGDGIINVWDIRKRDEGQLNQILAH
RNIVTQVRFSKEDGGKKLVSCGYDNLINVYSSDTWLKMGSLAGHTDKIISLDISNNSH
FLVSGGWDRSIKLWN"
gene complement(<893797..>895764)
/gene="HDA3"
/locus_tag="YPR179C"
/gene_synonym="PLO1"
/db_xref="GeneID:856309"
mRNA complement(<893797..>895764)
/gene="HDA3"
/locus_tag="YPR179C"
/gene_synonym="PLO1"
/product="Hda3p"
/transcript_id="NM_001184276.1"
/db_xref="GeneID:856309"
CDS complement(893797..895764)
/gene="HDA3"
/locus_tag="YPR179C"
/gene_synonym="PLO1"
/experiment="EXISTENCE:direct assay:GO:0003677 DNA binding
[PMID:19573535]"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:16415367]"
/experiment="EXISTENCE:direct assay:GO:0004407 histone
deacetylase activity [PMID:19573535]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0070823 HDA1
complex [PMID:11287668|PMID:8663039]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:11287668]"
/experiment="EXISTENCE:mutant phenotype:GO:0007059
chromosome segregation [PMID:16415367]"
/experiment="EXISTENCE:mutant phenotype:GO:0031047
regulatory ncRNA-mediated gene silencing [PMID:18022365]"
/experiment="EXISTENCE:physical interaction:GO:0070823
HDA1 complex [PMID:11287668]"
/note="Subunit of HDA1 histone deacetylase complex;
tetrameric trichostatin A-sensitive class II histone
deacetylase complex contains Hda1p homodimer and an
Hda2p-Hda3p heterodimer; required for activity of complex;
relocalizes to cytosol in response to hypoxia; similar to
Hda2p; the HDA1 histone deacetylase complex regulates
aging through trehalose metabolism"
/codon_start=1
/product="Hda3p"
/protein_id="NP_015505.1"
/db_xref="GeneID:856309"
/db_xref="SGD:S000006383"
/translation="MDLLRILDTKPIPTIVDATTLGISGNTSGDYWLPTTMSLYQKEL
TDQIVSLHYSDILRYFETSHYKEDVILESMKTMCLNGSLVATHPYLLIDHYMPKSLIT
RDVPAHLAENSGKFSVLRDLINLVQEYETETAIVCRPGRTMDLLEALLLGNKVHIKRY
DGHSIKSKQKANDFSCTVHLFSSEGINFTKYPIKSKARFDMLICLDTTVDTSQKDIQY
LLQYKRERKGLERYAPIVRLVAINSIDHCRLFFGKKFDKNSREYLENVTAAMVILRDR
LGTLPPDLRPIYSQKLHYLVEWLENPTVPWPLPDIYPLKQYTSMDVERSLLTEVHFKK
SDDQLEDAFSNCSKKRGRHGANKAASSTVAGIEDNITPSFYSTKRLKNDYYTNPLKQD
MTQLTGITTADNSSNVNYHLSSGIITHKLIQSMGEVYMDICVQKQELDDYSCLDDLQN
DHLKFFSNEDEKIIKEYETVLRTNNENLNRSHELEVENNLKFSQIETLEKDIETLKGS
LMAQGETLSKLKDAFVKTDNVQDEIEKEERVSVSRDTEKKYMEQEIKRAVDAIRENEE
ETHKLNEKQNGLESELKLKFEKSEISTKELNEKIGFLKKELKLENDLNEELVGQLSKT
MDNLENLTIPRVRTQNGNTKKKSRAKKPGNV"
gene <895961..>897004
/gene="AOS1"
/locus_tag="YPR180W"
/gene_synonym="RHC31"
/db_xref="GeneID:856310"
mRNA <895961..>897004
/gene="AOS1"
/locus_tag="YPR180W"
/gene_synonym="RHC31"
/product="E1 ubiquitin-activating protein AOS1"
/transcript_id="NM_001184277.1"
/db_xref="GeneID:856310"
CDS 895961..897004
/gene="AOS1"
/locus_tag="YPR180W"
/gene_synonym="RHC31"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0016925 protein
sumoylation [PMID:9312010]"
/experiment="EXISTENCE:direct assay:GO:0019948 SUMO
activating enzyme activity [PMID:9312010]"
/experiment="EXISTENCE:physical interaction:GO:0031510
SUMO activating enzyme complex [PMID:9312010]"
/note="Subunit of heterodimeric nuclear SUMO activating
enzyme E1 with Uba2p; activates Smt3p (SUMO) before its
conjugation to proteins (sumoylation), which may play a
role in protein targeting; essential for viability;
relocalizes to the cytosol in response to hypoxia"
/codon_start=1
/product="E1 ubiquitin-activating protein AOS1"
/protein_id="NP_015506.1"
/db_xref="GeneID:856310"
/db_xref="SGD:S000006384"
/translation="MDMKVEKLSEDEIALYDRQIRLWGMTAQANMRSAKVLLINLGAI
GSEITKSIVLSGIGHLTILDGHMVTEEDLGSQFFIGSEDVGQWKIDATKERIQDLNPR
IELNFDKQDLQEKDEEFFQQFDLVVATEMQIDEAIKINTLTRKLNIPLYVAGSNGLFA
YVFIDLIEFISEDEKLQSVRPTTVGPISSNRSIIEVTTRKDEEDEKKTYERIKTKNCY
RPLNEVLSTATLKEKMTQRQLKRVTSILPLTLSLLQYGLNQKGKAISFEQMKRDAAVW
CENLGVPATVVKDDYIQQFIKQKGIEFAPVAAIIGGAVAQDVINILGKRLSPLNNFIV
FDGITLDMPLFEF"
gene complement(<897361..>899667)
/gene="SEC23"
/locus_tag="YPR181C"
/db_xref="GeneID:856311"
mRNA complement(<897361..>899667)
/gene="SEC23"
/locus_tag="YPR181C"
/product="GTPase-activating protein SEC23"
/transcript_id="NM_001184278.1"
/db_xref="GeneID:856311"
CDS complement(897361..899667)
/gene="SEC23"
/locus_tag="YPR181C"
/experiment="EXISTENCE:direct assay:GO:0003400 regulation
of COPII vesicle coating [PMID:8451644]"
/experiment="EXISTENCE:direct assay:GO:0005096 GTPase
activator activity [PMID:8451644]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0030127 COPII
vesicle coat [PMID:8004676]"
/experiment="EXISTENCE:mutant phenotype:GO:0016236
macroautophagy [PMID:11694599]"
/experiment="EXISTENCE:mutant phenotype:GO:0061709
reticulophagy [PMID:31273116]"
/note="GTPase-activating protein, stimulates the GTPase
activity of Sar1p; component of the Sec23p-Sec24p
heterodimer of the COPII vesicle coat, involved in ER to
Golgi transport; substrate of Ubp3/Bre5 complex;
ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated
degradation of Sec23p is regulated by Cdc48p"
/codon_start=1
/product="GTPase-activating protein SEC23"
/protein_id="NP_015507.1"
/db_xref="GeneID:856311"
/db_xref="SGD:S000006385"
/translation="MDFETNEDINGVRFTWNVFPSTRSDANSNVVPVGCLYTPLKEYD
ELNVAPYNPVVCSGPHCKSILNPYCVIDPRNSSWSCPICNSRNHLPPQYTNLSQENMP
LELQSTTIEYITNKPVTVPPIFFFVVDLTSETENLDSLKESIITSLSLLPPNALIGLI
TYGNVVQLHDLSSETIDRCNVFRGDREYQLEALTEMLTGQKPTGPGGAASHLPNAMNK
VTPFSLNRFFLPLEQVEFKLNQLLENLSPDQWSVPAGHRPLRATGSALNIASLLLQGC
YKNIPARIILFASGPGTVAPGLIVNSELKDPLRSHHDIDSDHAQHYKKACKFYNQIAQ
RVAANGHTVDIFAGCYDQIGMSEMKQLTDSTGGVLLLTDAFSTAIFKQSYLRLFAKDE
EGYLKMAFNGNMAVKTSKDLKVQGLIGHASAVKKTDANNISESEIGIGATSTWKMASL
SPYHSYAIFFEIANTAANSNPMMSAPGSADRPHLAYTQFITTYQHSSGTNRIRVTTVA
NQLLPFGTPAIAASFDQEAAAVLMARIAVHKAETDDGADVIRWLDRTLIKLCQKYADY
NKDDPQSFRLAPNFSLYPQFTYYLRRSQFLSVFNNSPDETAFYRHIFTREDTTNSLIM
IQPTLTSFSMEDDPQPVLLDSISVKPNTILLLDTFFFILIYHGEQIAQWRKAGYQDDP
QYADFKALLEEPKLEAAELLVDRFPLPRFIDTEAGGSQARFLLSKLNPSDNYQDMARG
GSTIVLTDDVSLQNFMTHLQQVAVSGQA"
gene <900194..>900454
/gene="SMX3"
/locus_tag="YPR182W"
/db_xref="GeneID:856312"
mRNA <900194..>900454
/gene="SMX3"
/locus_tag="YPR182W"
/product="mRNA splicing protein SMX3"
/transcript_id="NM_001184279.1"
/db_xref="GeneID:856312"
CDS 900194..900454
/gene="SMX3"
/locus_tag="YPR182W"
/experiment="EXISTENCE:curator inference:GO:0000398 mRNA
splicing, via spliceosome []"
/experiment="EXISTENCE:direct assay:GO:0005682 U5 snRNP
[PMID:11720285|PMID:11720284]"
/experiment="EXISTENCE:direct assay:GO:0005685 U1 snRNP
[PMID:9630245]"
/experiment="EXISTENCE:direct assay:GO:0005686 U2 snRNP
[PMID:9012791]"
/experiment="EXISTENCE:direct assay:GO:0008266 poly(U) RNA
binding [PMID:12618433]"
/experiment="EXISTENCE:direct assay:GO:0046540 U4/U6 x U5
tri-snRNP complex [PMID:10377396|PMID:10449419]"
/experiment="EXISTENCE:physical interaction:GO:1990935
splicing factor binding [PMID:9528767]"
/note="Core Sm protein Sm F; part of heteroheptameric
complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and
Smx2p) that is part of the spliceosomal U1, U2, U4, and U5
snRNPs; homolog of human Sm F"
/codon_start=1
/product="mRNA splicing protein SMX3"
/protein_id="NP_015508.1"
/db_xref="GeneID:856312"
/db_xref="SGD:S000006386"
/translation="MSESSDISAMQPVNPKPFLKGLVNHRVGVKLKFNSTEYRGTLVS
TDNYFNLQLNEAEEFVAGVSHGTLGEIFIRCNNVLYIRELPN"
gene <900755..>901558
/gene="DPM1"
/locus_tag="YPR183W"
/gene_synonym="SED3"
/db_xref="GeneID:856313"
mRNA <900755..>901558
/gene="DPM1"
/locus_tag="YPR183W"
/gene_synonym="SED3"
/product="dolichyl-phosphate beta-D-mannosyltransferase"
/transcript_id="NM_001184280.1"
/db_xref="GeneID:856313"
CDS 900755..901558
/gene="DPM1"
/locus_tag="YPR183W"
/gene_synonym="SED3"
/EC_number="2.4.1.83"
/experiment="EXISTENCE:direct assay:GO:0004582
dolichyl-phosphate beta-D-mannosyltransferase activity
[PMID:16549409|PMID:3053713]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:16407407]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762|PMID:1803815]"
/experiment="EXISTENCE:direct assay:GO:0042175 nuclear
outer membrane-endoplasmic reticulum membrane network
[PMID:10419517]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0006488
dolichol-linked oligosaccharide biosynthetic process
[PMID:2146492]"
/experiment="EXISTENCE:mutant phenotype:GO:0006506 GPI
anchor biosynthetic process [PMID:2146492]"
/note="Dolichol phosphate mannose (Dol-P-Man) synthase of
ER membrane; catalyzes formation of Dol-P-Man from Dol-P
and GDP-Man; required for biosynthesis of glycosyl
phosphatidylinositol (GPI) membrane anchor, as well as
O-mannosylation and protein N- and O-linked glycosylation;
human homolog DPM1 can complement yeast mutant strains"
/codon_start=1
/product="dolichyl-phosphate beta-D-mannosyltransferase"
/protein_id="NP_015509.1"
/db_xref="GeneID:856313"
/db_xref="SGD:S000006387"
/translation="MSIEYSVIVPAYHEKLNIKPLTTRLFAGMSPEMAKKTELIFVDD
NSQDGSVEEVDALAHQGYNVRIIVRTNERGLSSAVLKGFYEAKGQYLVCMDADLQHPP
ETVPKLFESLHDHAFTLGTRYAPGVGIDKDWPMYRRVISSTARMMARPLTIASDPMSG
FFGLQKKYLENCNPRDINSQGFKIALELLAKLPLPRDPRVAIGEVPFTFGVRTEGESK
LSGKVIIQYLQQLKELYVFKFGANNLILFITFWSILFFYVCYQLYHLVF"
gene <902044..>906654
/gene="GDB1"
/locus_tag="YPR184W"
/db_xref="GeneID:856314"
mRNA <902044..>906654
/gene="GDB1"
/locus_tag="YPR184W"
/product="bifunctional
4-alpha-glucanotransferase/amylo-alpha-1,6-glucosidase"
/transcript_id="NM_001184281.1"
/db_xref="GeneID:856314"
CDS 902044..906654
/gene="GDB1"
/locus_tag="YPR184W"
/EC_number="2.4.1.25"
/EC_number="3.2.1.33"
/experiment="EXISTENCE:direct assay:GO:0004134
4-alpha-glucanotransferase activity [PMID:11094287]"
/experiment="EXISTENCE:direct assay:GO:0004135
amylo-alpha-1,6-glucosidase activity [PMID:11094287]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:mutant phenotype:GO:0004134
4-alpha-glucanotransferase activity [PMID:11094287]"
/experiment="EXISTENCE:mutant phenotype:GO:0004135
amylo-alpha-1,6-glucosidase activity [PMID:11094287]"
/experiment="EXISTENCE:mutant phenotype:GO:0005980
glycogen catabolic process [PMID:11094287]"
/note="Glycogen debranching enzyme; contains
glucanotranferase and alpha-1,6-amyloglucosidase
activities; required for glycogen degradation;
phosphorylated in mitochondria; activity is inhibited by
Igd1p; protein abundance increases in response to DNA
replication stress"
/codon_start=1
/product="bifunctional
4-alpha-glucanotransferase/amylo-alpha-1,6-glucosidase"
/protein_id="NP_015510.1"
/db_xref="GeneID:856314"
/db_xref="SGD:S000006388"
/translation="MNRSLLLRLSDTGEPITSCSYGKGVLTLPPIPLPKDAPKDQPLY
TVKLLVSAGSPVARDGLVWTNCPPDHNTPFKRDKFYKKIIHSSFHEDDCIDLNVYAPG
SYCFYLSFRNDNEKLETTRKYYFVALPMLYINDQFLPLNSIALQSVVSKWLGSDWEPI
LSKIAAKNYNMVHFTPLQERGESNSPYSIYDQLQFDQEHFKSPEDVKNLVEHIHRDLN
MLSLTDIVFNHTANNSPWLVEHPEAGYNHITAPHLISAIELDQELLNFSRNLKSWGYP
TELKNIEDLFKIMDGIKVHVLGSLKLWEYYAVNVQTALRDIKAHWNDESNESYSFPEN
IKDISSDFVKLASFVKDNVTEPNFGTLGERNSNRINVPKFIQLLKLINDGGSDDSESS
LATAQNILNEVNLPLYREYDDDVSEILEQLFNRIKYLRLDDGGPKQGPVTVDVPLTEP
YFTRFKGKDGTDYALANNGWIWNGNPLVDFASQNSRAYLRREVIVWGDCVKLRYGKSP
EDSPYLWERMSKYIEMNAKIFDGFRIDNCHSTPIHVGEYFLDLARKYNPNLYVVAELF
SGSETLDCLFVERLGISSLIREAMQAWSEEELSRLVHKHGGRPIGSYKFVPMDDFSYP
ADINLNEEHCFNDSNDNSIRCVSEIMIPKILTATPPHALFMDCTHDNETPFEKRTVED
TLPNAALVALCSSAIGSVYGYDEIFPHLLNLVTEKRHYDISTPTGSPSIGITKVKATL
NSIRTSIGEKAYDIEDSEMHVHHQGQYITFHRMDVKSGKGWYLIARMKFSDNDDPNET
LPPVVLNQSTCSLRFSYALERVGDEIPNDDKFIKGIPTKLKELEGFDISYDDSKKIST
IKLPNEFPQGSIAIFETQQNGVDESLDHFIRSGALKATSSLTLESINSVLYRSEPEEY
DVSAGEGGAYIIPNFGKPVYCGLQGWVSVLRKIVFYNDLAHPLSANLRNGHWALDYTI
SRLNYYSDEAGINEVQNWLRSRFDRVKKLPSYLVPSYFALIIGILYGCCRLKAIQLMS
RNIGKSTLFVQSLSMTSIQMVSRMKSTSILPGENVPSMAAGLPHFSVNYMRCWGRDVF
ISLRGMLLTTGRFDEAKAHILAFAKTLKHGLIPNLLDAGRNPRYNARDAAWFFLQAVQ
DYVYIVPDGEKILQEQVTRRFPLDDTYIPVDDPRAFSYSSTLEEIIYEILSRHAKGIK
FREANAGPNLDRVMTDKGFNVEIHVDWSTGLIHGGSQYNCGTWMDKMGESEKAGSVGI
PGTPRDGAAIEINGLLKSALRFVIELKNKGLFKFSDVETQDGGRIDFTEWNQLLQDNF
EKRYYVPEDPSQDADYDVSAKLGVNRRGIYRDLYKSGKPYEDYQLRPNFAIAMTVAPE
LFVPEHAIKAITIADEVLRGPVGMRTLDPSDYNYRPYYNNGEDSDDFATSKGRNYHQG
PEWVWLYGYFLRAFHHFHFKTSPRCQNAAKEKPSSYLYQQLYYRLKGHRKWIFESVWA
GLTELTNKDGEVCNDSSPTQAWSSACLLDLFYDLWDAYEDDS"
regulatory 907211..907351
/regulatory_class="other"
/note="Upstream open reading frame (uORF) in 5'
untranslated region of ATG13 gene, regulate translation"
gene <907218..>909434
/gene="ATG13"
/locus_tag="YPR185W"
/gene_synonym="APG13"
/db_xref="GeneID:856315"
mRNA <907218..>909434
/gene="ATG13"
/locus_tag="YPR185W"
/gene_synonym="APG13"
/product="serine/threonine protein kinase regulatory
subunit ATG13"
/transcript_id="NM_001184282.1"
/db_xref="GeneID:856315"
CDS 907218..909434
/gene="ATG13"
/locus_tag="YPR185W"
/gene_synonym="APG13"
/experiment="EXISTENCE:direct assay:GO:0000045
autophagosome assembly [PMID:18725539]"
/experiment="EXISTENCE:direct assay:GO:0000407 phagophore
assembly site [PMID:29540529]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0019887 protein
kinase regulator activity [PMID:22885598]"
/experiment="EXISTENCE:direct assay:GO:0070016 armadillo
repeat domain binding [PMID:31512555]"
/experiment="EXISTENCE:direct assay:GO:0120095
vacuole-isolation membrane contact site [PMID:23549786]"
/experiment="EXISTENCE:direct assay:GO:1990316 Atg1/ULK1
kinase complex [PMID:26753620]"
/experiment="EXISTENCE:genetic interaction:GO:0034497
protein localization to phagophore assembly site
[PMID:23509291]"
/experiment="EXISTENCE:mutant phenotype:GO:0000422
autophagy of mitochondrion [PMID:19793921]"
/experiment="EXISTENCE:mutant phenotype:GO:0006914
autophagy [PMID:32508216]"
/experiment="EXISTENCE:mutant phenotype:GO:0006995
cellular response to nitrogen starvation [PMID:18725539]"
/experiment="EXISTENCE:mutant phenotype:GO:0016236
macroautophagy [PMID:9224892]"
/experiment="EXISTENCE:mutant phenotype:GO:0019887 protein
kinase regulator activity [PMID:10995454]"
/experiment="EXISTENCE:mutant phenotype:GO:0032258
cytoplasm to vacuole targeting by the Cvt pathway
[PMID:27768871]"
/experiment="EXISTENCE:mutant phenotype:GO:0034727
piecemeal microautophagy of the nucleus [PMID:18701704]"
/experiment="EXISTENCE:mutant phenotype:GO:0044804
nucleophagy [PMID:22768199]"
/experiment="EXISTENCE:mutant phenotype:GO:0071211 protein
targeting to vacuole involved in autophagy
[PMID:27768871]"
/experiment="EXISTENCE:mutant phenotype:GO:0071255 Cvt
vesicle assembly [PMID:10837477]"
/experiment="EXISTENCE:physical interaction:GO:1990316
Atg1/ULK1 kinase complex [PMID:15743910]"
/note="Regulatory subunit of the Atg1p signaling complex;
stimulates Atg1p kinase activity; required for vesicle
formation during autophagy and the cytoplasm-to-vacuole
targeting (Cvt) pathway; contains a HORMA domain required
for autophagy and for recruitment of the
phosphatidylinositol 3-kinase complex subunit Atg14p to
the pre-autophagosomal structure"
/codon_start=1
/product="serine/threonine protein kinase regulatory
subunit ATG13"
/protein_id="NP_015511.1"
/db_xref="GeneID:856315"
/db_xref="SGD:S000006389"
/translation="MVAEEDIEKQVLQLIDSFFLKTTLLICSTESSRYQSSTENIFLF
DDTWFEDHSELVSELPEIISKWSHYDGRKELPPLVVETYLDLRQLNSSHLVRLKDHEG
HLWNVCKGTKKQEIVMERWLIELDNSSPTFKSYSEDETDVNELSKQLVLLFRYLLTLI
QLLPTTELYQLLIKSYNGPQNEGSSNPITSTGPLVSIRTCVLDGSKPILSKGRIGLSK
PIINTYSNALNESNLPAHLDQKKITPVWTKFGLLRVSVSYRRDWKFEINNTNDELFSA
RHASVSHNSQGPQNQPEQEGQSDQDIGKRQPQFQQQQQPQQQQQQQQQQQRQHQVQTQ
QQRQIPDRRSLSLSPCTRANSFEPQSWQKKVYPISRPVQPFKVGSIGSQSASRNPSNS
SFFNQPPVHRPSMSSNYGPQMNIEGTSVGSTSKYSSSFGNIRRHSSVKTTENAEKVSK
AVKSPLQPQESQEDLMDFVKLLEEKPDLTIKKTSGNNPPNINISDSLIRYQNLKPSND
LLSEDLSVSLSMDPNHTYHRGRSDSHSPLPSISPSMHYGSLNSRMSQGANASHLIARG
GGNSSTSALNSRRNSLDKSSNKQGMSGLPPIFGGESTSYHHDNKIQKYNQLGVEEDDD
DENDRLLNQMGNSATKFKSSISPRSIDSISSSFIKSRIPIRQPYHYSQPTTAPFQAQA
KFHKPANKLIDNGNRSNSNNNNHNGNDAVGVMHNDEDDQDDDLVFFMSDMNLSKEG"
gene complement(<909733..>911022)
/gene="PZF1"
/locus_tag="YPR186C"
/gene_synonym="TFC2"
/db_xref="GeneID:856316"
mRNA complement(<909733..>911022)
/gene="PZF1"
/locus_tag="YPR186C"
/gene_synonym="TFC2"
/product="Pzf1p"
/transcript_id="NM_001184283.1"
/db_xref="GeneID:856316"
CDS complement(909733..911022)
/gene="PZF1"
/locus_tag="YPR186C"
/gene_synonym="TFC2"
/experiment="EXISTENCE:curator inference:GO:0005634
nucleus [PMID:2642897]"
/experiment="EXISTENCE:direct assay:GO:0001002 RNA
polymerase III type 1 promoter sequence-specific DNA
binding [PMID:2642897]"
/experiment="EXISTENCE:direct assay:GO:0001010 RNA
polymerase II sequence-specific DNA-binding transcription
factor recruiting activity [PMID:8496187]"
/experiment="EXISTENCE:direct assay:GO:0042791 5S class
rRNA transcription by RNA polymerase III [PMID:2642897]"
/experiment="EXISTENCE:mutant phenotype:GO:0001010 RNA
polymerase II sequence-specific DNA-binding transcription
factor recruiting activity [PMID:8496187]"
/note="Transcription factor IIIA (TFIIIA); essential DNA
binding protein required for transcription of 5S rRNA by
RNA polymerase III; not involved in transcription of other
RNAP III genes; nine conserved zinc fingers; may also bind
5S rRNA"
/codon_start=1
/product="Pzf1p"
/protein_id="NP_015512.1"
/db_xref="GeneID:856316"
/db_xref="SGD:S000006390"
/translation="MGGEVLNNEGMPLAELKQETIPISRSESSESLNSLTSTRSSSSN
RPKTYFCDYDGCDKAFTRPSILTEHQLSVHQGLRAFQCDKCAKSFVKKSHLERHLYTH
SDTKPFQCSYCGKGVTTRQQLKRHEVTHTKSFICPEEGCNLRFYKHPQLRAHILSVHL
HKLTCPHCNKSFQRPYRLRNHISKHHDPEVENPYQCTFAGCCKEFRIWSQLQSHIKND
HPKLKCPICSKPCVGENGLQMHMIIHDDSLVTKNWKCHICPDMSFSRKHDLLTHYGSI
HTEEDIPLELKYKISDIQQLVQDHGVQLGNSKHSNEQDEEKISNRLRKRRKLTENNNV
EFLQNEVDLEKRLESGENGLNLLLNTVGRKYRCFYNNCSRTFKTKEKYEKHIDKHKVH
ELKLKILQEKEENKTLVDQNHKEPFIIQKETQSAGDK"
gene <911257..>911800
/gene="RPO26"
/locus_tag="YPR187W"
/gene_synonym="RPB6"
/db_xref="GeneID:856317"
mRNA join(<911257..911276,911353..>911800)
/gene="RPO26"
/locus_tag="YPR187W"
/gene_synonym="RPB6"
/product="DNA-directed RNA polymerase core subunit RPO26"
/transcript_id="NM_001184284.1"
/db_xref="GeneID:856317"
CDS join(911257..911276,911353..911800)
/gene="RPO26"
/locus_tag="YPR187W"
/gene_synonym="RPB6"
/experiment="EXISTENCE:direct assay:GO:0003899
DNA-directed RNA polymerase activity
[PMID:9837969|PMID:8288647|PMID:3905793]"
/experiment="EXISTENCE:direct assay:GO:0003968
RNA-directed RNA polymerase activity [PMID:18004386]"
/experiment="EXISTENCE:direct assay:GO:0005665 RNA
polymerase II, core complex
[PMID:2183013|PMID:2186966|PMID:1331084]"
/experiment="EXISTENCE:direct assay:GO:0005666 RNA
polymerase III complex [PMID:10611227|PMID:3905793]"
/experiment="EXISTENCE:direct assay:GO:0005736 RNA
polymerase I complex [PMID:9837969|PMID:11486042]"
/experiment="EXISTENCE:direct assay:GO:0006360
transcription by RNA polymerase I [PMID:9837969]"
/experiment="EXISTENCE:direct assay:GO:0042797 tRNA
transcription by RNA polymerase III [PMID:3905793]"
/experiment="EXISTENCE:mutant phenotype:GO:0006366
transcription by RNA polymerase II [PMID:12697831]"
/experiment="EXISTENCE:mutant phenotype:GO:0006383
transcription by RNA polymerase III [PMID:12697831]"
/note="RNA polymerase subunit ABC23; common to RNA
polymerases I, II, and III; part of central core; similar
to bacterial omega subunit"
/codon_start=1
/product="DNA-directed RNA polymerase core subunit RPO26"
/protein_id="NP_015513.1"
/db_xref="GeneID:856317"
/db_xref="SGD:S000006391"
/translation="MSDYEEAFNDGNENFEDFDVEHFSDEETYEEKPQFKDGETTDAN
GKTIVTGGNGPEDFQQHEQIRRKTLKEKAIPKDQRATTPYMTKYERARILGTRALQIS
MNAPVFVDLEGETDPLRIAMKELAEKKIPLVIRRYLPDGSFEDWSVEELIVDL"
gene complement(<911988..>912479)
/gene="MLC2"
/locus_tag="YPR188C"
/db_xref="GeneID:856318"
mRNA complement(<911988..>912479)
/gene="MLC2"
/locus_tag="YPR188C"
/product="Mlc2p"
/transcript_id="NM_001184285.1"
/db_xref="GeneID:856318"
CDS complement(911988..912479)
/gene="MLC2"
/locus_tag="YPR188C"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:15210731]"
/experiment="EXISTENCE:direct assay:GO:0000142 cellular
bud neck contractile ring [PMID:15210731]"
/experiment="EXISTENCE:genetic interaction:GO:1902404
mitotic actomyosin contractile ring contraction
[PMID:19109423]"
/experiment="EXISTENCE:mutant phenotype:GO:1902404 mitotic
actomyosin contractile ring contraction [PMID:15210731]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:15210731]"
/experiment="EXISTENCE:physical interaction:GO:0016460
myosin II complex [PMID:15210731]"
/experiment="EXISTENCE:physical interaction:GO:0032038
myosin II heavy chain binding [PMID:15210731]"
/note="Regulatory light chain for the type II myosin
Myo1p; binds to an IQ motif of Myo1p, localization to the
bud neck depends on Myo1p; involved in the disassembly of
the Myo1p ring"
/codon_start=1
/product="Mlc2p"
/protein_id="NP_015514.1"
/db_xref="GeneID:856318"
/db_xref="SGD:S000006392"
/translation="MDHSESLTFNQLTQDYINKLKDAFQMLDEDEDGLISRGDLTKIY
ATLGKTLTDEEWSKMVPDNDTSTAEVGEEGVSFPIFLSIMGKNLSQFPEREELEESLK
AIGRGHDLNVPLNEVIDSLKEAGFENPEEEFAKLFKLFTTNQQATEERTFRGKLFLDS
ITD"
gene <912664..>916962
/gene="SKI3"
/locus_tag="YPR189W"
/gene_synonym="SKI5"
/db_xref="GeneID:856319"
mRNA <912664..>916962
/gene="SKI3"
/locus_tag="YPR189W"
/gene_synonym="SKI5"
/product="SKI complex subunit tetratricopeptide repeat
protein SKI3"
/transcript_id="NM_001184286.1"
/db_xref="GeneID:856319"
CDS 912664..916962
/gene="SKI3"
/locus_tag="YPR189W"
/gene_synonym="SKI5"
/experiment="EXISTENCE:direct assay:GO:0055087 Ski complex
[PMID:18042677|PMID:10744028]"
/experiment="EXISTENCE:genetic interaction:GO:0070478
nuclear-transcribed mRNA catabolic process, 3'-5'
exonucleolytic nonsense-mediated decay [PMID:12881429]"
/experiment="EXISTENCE:mutant phenotype:GO:0000956
nuclear-transcribed mRNA catabolic process
[PMID:10611222|PMID:9482746]"
/experiment="EXISTENCE:mutant phenotype:GO:0070478
nuclear-transcribed mRNA catabolic process, 3'-5'
exonucleolytic nonsense-mediated decay [PMID:12881429]"
/experiment="EXISTENCE:mutant phenotype:GO:0070481
nuclear-transcribed mRNA catabolic process, non-stop decay
[PMID:17660569|PMID:11910110]"
/experiment="EXISTENCE:physical interaction:GO:0055087 Ski
complex [PMID:18042677]"
/note="Ski complex component and TPR protein; mediates
3'-5' RNA degradation by the cytoplasmic exosome; null
mutants have superkiller phenotype of increased viral
dsRNAs and are synthetic lethal with mutations in 5'-3'
mRNA decay; role in preventing L-A mycovirus pathogenesis;
mutations in the human ortholog, TTC37, causes Syndromic
diarrhea/Trichohepatoenteric (SD/THE) syndrome"
/codon_start=1
/product="SKI complex subunit tetratricopeptide repeat
protein SKI3"
/protein_id="NP_015515.1"
/db_xref="GeneID:856319"
/db_xref="SGD:S000006393"
/translation="MSDIKQLLKEAKQELTNRDYEETIEISEKVLKLDPDNYFAHIFL
GKALSSLPASNNVSSNRNLERATNHYVSAAKLVPDNLLAWKGLFLLFRTTEVVPDILS
YDEYFDLCGQYADALLKQEQSQVELINDIKLLKKTHPDCQKAFYQHLKPGSLMAETIG
RHLSTPQDALLNLIKILSNIETTEIGKTLSQNRLKLKASDPDYQIKLNSFSWEIIKNS
EIDQLYNQLVNILADDQKRSEIENQWLEYRIKVLKSMPLDVKKDFFTKVKEMVEDMVL
VNHQSLLAWQKYFEWTDYEDLDNMDAPLIIKYFKKFPKDPLAMILYSWLSSKLSKYDI
KSLESANKPPEGHKKTEKETDIKDVDETNEDEVKDRVEDEVKDRVEDEVKDQDEEAKE
DEEEDLDDIEIGLLEEEVVTVLTENIVKCKNNILAHRILCQYYLLTKEYEAALPYIKN
GISLIAYNIKDLGVHLPLTKREFSLDLATVYTYVDAPKDHNAALKLYDNILSGDFSNI
QAKMGKGIIFIERKNWKDAMTLLTQVHEQSPNNLEVLSELSWSKAHMGYMDEALAGLD
TVIKGIKGMDLRSIDFRALNLWRQAKVYIMKHASINDAKQENVKCAFKLLIQSIKILD
TFAPGFSTLGDIYCHYYKDHLRAFKCYFKAFDLDAGDYTAAKYITETYASKPNWQAAS
SIASRLIKGEKAKAELRSNNWPFRVVGIAHLEKQEESDSIEWFQSALRVDPNDVESWV
GLGQAYHACGRIEASIKVFDKAIQLRPSHTFAQYFKAISLCDVGEYLESLDILEKVCQ
EAATEESFQIGLVEVLMRCSLDLYSQGFLLKSVSIAKDTIERIKIIISELKCENQQVW
IYLSQVLRLFIWIESKVDTLPVESLVSIFENSQFSGSEEIDSVDNIKIDTLLDSTTDD
NVSIACKFLILASKYSVSDQKFTDIAGTVRASYWYNIGISELTAFITLKEPQYRDAAI
FAFKKSIQLQSNTSETWIGLGIATMDINFRVSQHCFIKATALEPKATNTWFNLAMLGL
KKKDTEFAQQVLNKLQSLAPQDSSPWLGMALILEEQGDIIGSSKLFAHSFILSNGRSK
AAQFMYAKNVLENHINNGDDERDIETVEKLTTASIALEQFFKKSPDSQFALQCALLTL
ERLHHYENANELANRLIGILEKKFEKTQDERELFNFAIIKGQFARIHLGLGNFELSIE
NADLSQGIISESSDEKSMKTKISNHICLGLSYFFLNDFDQTLNQFQELLSISKDSKHL
VVLIAKVLYDVGESDTKEIALQELTEYIATSGADLLVTLTIAAMSILDDKREDLSIIL
EELKALPLSKQIIDKHKDAPYLIEEITKRLYRNDTGKQVWQRSAYFFPNNLKVWERLD
KNIQRRIASNGQNKVTAEEMSKLYCESKNLRSIQRGMFLCPWNVTAVKALNECF"
gene complement(<917077..>919041)
/gene="RPC82"
/locus_tag="YPR190C"
/gene_synonym="RPC3; RPC80"
/db_xref="GeneID:856320"
mRNA complement(<917077..>919041)
/gene="RPC82"
/locus_tag="YPR190C"
/gene_synonym="RPC3; RPC80"
/product="DNA-directed RNA polymerase III subunit C82"
/transcript_id="NM_001184287.1"
/db_xref="GeneID:856320"
CDS complement(917077..919041)
/gene="RPC82"
/locus_tag="YPR190C"
/gene_synonym="RPC3; RPC80"
/experiment="EXISTENCE:direct assay:GO:0003899
DNA-directed RNA polymerase activity [PMID:3905793]"
/experiment="EXISTENCE:direct assay:GO:0005666 RNA
polymerase III complex [PMID:3905793|PMID:10611227]"
/experiment="EXISTENCE:direct assay:GO:0042797 tRNA
transcription by RNA polymerase III [PMID:3905793]"
/experiment="EXISTENCE:mutant phenotype:GO:0042797 tRNA
transcription by RNA polymerase III [PMID:1406632]"
/note="RNA polymerase III subunit C82"
/codon_start=1
/product="DNA-directed RNA polymerase III subunit C82"
/protein_id="NP_015516.1"
/db_xref="GeneID:856320"
/db_xref="SGD:S000006394"
/translation="MDELLGEALSAENQTGESTVESEKLVTPEDVMTISSLEQRTLNP
DLFLYKELVKAHLGERAASVIGMLVALGRLSVRELVEKIDGMDVDSVKTTLVSLTQLR
CVKYLQETAISGKKTTYYYYNEEGIHILLYSGLIIDEIITQMRVNDEEEHKQLVAEIV
QNVISLGSLTVEDYLSSVTSDSMKYTISSLFVQLCEMGYLIQISKLHYTPIEDLWQFL
YEKHYKNIPRNSPLSDLKKRSQAKMNAKTDFAKIINKPNELSQILTVDPKTSLRIVKP
TVSLTINLDRFMKGRRSKQLINLAKTRVGSVTAQVYKIALRLTEQKSPKIRDPLTQTG
LLQDLEEAKSFQDEAELVEEKTPGLTFNAIDLARHLPAELDLRGSLLSRKPSDNKKRS
GSNAAASLPSKKLKTEDGFVIPALPAAVSKSLQESGDTQEEDEEEEDLDADTEDPHSA
SLINSHLKILASSNFPFLNETKPGVYYVPYSKLMPVLKSSVYEYVIASTLGPSAMRLS
RCIRDNKLVSEKIINSTALMKEKDIRSTLASLIRYNSVEIQEVPRTADRSASRAVFLF
RCKETHSYNFMRQNLEWNMANLLFKKEKLKQENSTLLKKANRDDVKGRENELLLPSEL
NQLKMVNERELNVFARLSRLLSLWEVFQMA"
gene <919381..>920487
/gene="QCR2"
/locus_tag="YPR191W"
/gene_synonym="COR2; UCR2"
/db_xref="GeneID:856321"
mRNA <919381..>920487
/gene="QCR2"
/locus_tag="YPR191W"
/gene_synonym="COR2; UCR2"
/product="ubiquinol--cytochrome-c reductase subunit 2"
/transcript_id="NM_001184288.1"
/db_xref="GeneID:856321"
CDS 919381..920487
/gene="QCR2"
/locus_tag="YPR191W"
/gene_synonym="COR2; UCR2"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:11914276|PMID:16823961|PMID:14576278|PMID:24769239|P
MID:11502169]"
/experiment="EXISTENCE:direct assay:GO:0006122
mitochondrial electron transport, ubiquinol to cytochrome
c [PMID:3028797]"
/experiment="EXISTENCE:direct assay:GO:0030061
mitochondrial crista [PMID:19019989]"
/experiment="EXISTENCE:direct assay:GO:0045275 respiratory
chain complex III [PMID:10873857]"
/experiment="EXISTENCE:mutant phenotype:GO:0008121
quinol-cytochrome-c reductase activity [PMID:3028797]"
/experiment="EXISTENCE:mutant phenotype:GO:0009060 aerobic
respiration [PMID:3028797]"
/note="Subunit 2 of ubiquinol cytochrome-c reductase
(Complex III); Complex III is a component of the
mitochondrial inner membrane electron transport chain;
phosphorylated; transcription is regulated by Hap1p,
Hap2p/Hap3p, and heme"
/codon_start=1
/product="ubiquinol--cytochrome-c reductase subunit 2"
/protein_id="NP_015517.1"
/db_xref="GeneID:856321"
/db_xref="SGD:S000006395"
/translation="MLSAARLQFAQGSVRRLTVSARDAPTKISTLAVKVHGGSRYATK
DGVAHLLNRFNFQNTNTRSALKLVRESELLGGTFKSTLDREYITLKATFLKDDLPYYV
NALADVLYKTAFKPHELTESVLPAARYDYAVAEQCPVKSAEDQLYAITFRKGLGNPLL
YDGVERVSLQDIKDFADKVYTKENLEVSGENVVEADLKRFVDESLLSTLPAGKSLVSK
SEPKFFLGEENRVRFIGDSVAAIGIPVNKASLAQYEVLANYLTSALSELSGLISSAKL
DKFTDGGLFTLFVRDQDSAVVSSNIKKIVADLKKGKDLSPAINYTKLKNAVQNESVSS
PIELNFDAVKDFKLGKFNYVAVGDVSNLPYLDEL"
gene <921860..>922777
/gene="AQY1"
/locus_tag="YPR192W"
/db_xref="GeneID:856322"
mRNA <921860..>922777
/gene="AQY1"
/locus_tag="YPR192W"
/product="Aqy1p"
/transcript_id="NM_001184289.1"
/db_xref="GeneID:856322"
CDS 921860..922777
/gene="AQY1"
/locus_tag="YPR192W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:10092523]"
/experiment="EXISTENCE:direct assay:GO:0055085
transmembrane transport [PMID:9765289]"
/experiment="EXISTENCE:genetic interaction:GO:0015250
water channel activity [PMID:11158584]"
/experiment="EXISTENCE:genetic interaction:GO:0055085
transmembrane transport [PMID:11158584]"
/experiment="EXISTENCE:mutant phenotype:GO:0006833 water
transport [PMID:9765289]"
/experiment="EXISTENCE:mutant phenotype:GO:0015250 water
channel activity [PMID:9765289]"
/experiment="EXISTENCE:mutant phenotype:GO:0030437
ascospore formation [PMID:15583134]"
/note="Spore-specific water channel; mediates the
transport of water across cell membranes, developmentally
controlled; may play a role in spore maturation, probably
by allowing water outflow, may be involved in freeze
tolerance"
/codon_start=1
/product="Aqy1p"
/protein_id="NP_015518.1"
/db_xref="GeneID:856322"
/db_xref="SGD:S000006396"
/translation="MSSNDSNDTDKQHTRLDPTGVDDAYIPPEQPETKHHRFKISRDT
LRDHFIAAVGEFCGTFMFLWCAYVICNVANHDVALVAAPDGSHPGQLIMIAIGFGFSV
MFSIWCFAGVSGGALNPAMSLSLCLARAVSPTRCVVMWVSQIVAGMAAGGAASAMTPG
EVLFANSLGLGCSRTRGLFLEMFGTAILCLTVLMTAVEKRETNFMAALPIGISLFIAH
VALTAYTGTGVNPARSLGAAVAARYFPHYHWIYWIGTLLGSILAWSVWQLLQILDYTT
YVTAEKAASTKEKAQKKGETSSSSAVAEV"
gene complement(<922909..>923379)
/gene="HPA2"
/locus_tag="YPR193C"
/gene_synonym="KAT10"
/db_xref="GeneID:856323"
mRNA complement(<922909..>923379)
/gene="HPA2"
/locus_tag="YPR193C"
/gene_synonym="KAT10"
/product="histone acetyltransferase"
/transcript_id="NM_001184290.1"
/db_xref="GeneID:856323"
CDS complement(922909..923379)
/gene="HPA2"
/locus_tag="YPR193C"
/gene_synonym="KAT10"
/EC_number="2.3.1.48"
/experiment="EXISTENCE:direct assay:GO:0004059
aralkylamine N-acetyltransferase activity [PMID:40551389]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0006473 protein
acetylation [PMID:23775086]"
/experiment="EXISTENCE:direct assay:GO:0008080
N-acetyltransferase activity [PMID:23775086]"
/note="Tetrameric histone acetyltransferase; has
similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates
histones H3 and H4 in vitro and exhibits autoacetylation
activity; contains aralkylamine N-acetyltransferase
activity in vitro; also acetylates polyamines"
/codon_start=1
/product="histone acetyltransferase"
/protein_id="NP_015519.1"
/db_xref="GeneID:856323"
/db_xref="SGD:S000006397"
/translation="MSNTSEDNITVRFVTENDKEGWQRLWKSYQDFYEVSFPDDLDDF
NFGRFLDPNIKMWAAVAVESSSEKIIGMINFFNHMTTWDFKDKIYINDLYVDENSRVK
GAGGKLIQFVYDEADKLGTPSVYWCTDESNHRAQLLYVKVGYKAPKILYKRKGY"
gene complement(<924304..>926937)
/gene="OPT2"
/locus_tag="YPR194C"
/db_xref="GeneID:856324"
mRNA complement(<924304..>926937)
/gene="OPT2"
/locus_tag="YPR194C"
/product="Opt2p"
/transcript_id="NM_001184291.1"
/db_xref="GeneID:856324"
CDS complement(924304..926937)
/gene="OPT2"
/locus_tag="YPR194C"
/experiment="EXISTENCE:direct assay:GO:0000138 Golgi trans
cisterna [PMID:25359886]"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:25130273]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:25359886]"
/experiment="EXISTENCE:direct assay:GO:0006857
oligopeptide transport [PMID:9643541]"
/experiment="EXISTENCE:direct assay:GO:0035673
oligopeptide transmembrane transporter activity
[PMID:9643541]"
/experiment="EXISTENCE:genetic interaction:GO:0044088
regulation of vacuole organization [PMID:25359886]"
/experiment="EXISTENCE:genetic interaction:GO:0061091
regulation of phospholipid translocation [PMID:25359886]"
/experiment="EXISTENCE:mutant phenotype:GO:0042144 vacuole
fusion, non-autophagic [PMID:19767828]"
/experiment="EXISTENCE:mutant phenotype:GO:0045454 cell
redox homeostasis [PMID:25130273]"
/experiment="EXISTENCE:mutant phenotype:GO:0051515
positive regulation of monopolar cell growth
[PMID:25359886]"
/note="Oligopeptide transporter; localized to peroxisomes
and affects glutathione redox homeostasis; also localizes
to the plasma membrane (PM) and to the late Golgi, and has
a role in maintenance of lipid asymmetry between the inner
and outer leaflets of the PM; member of the OPT family,
with potential orthologs in S. pombe and C. albicans; also
plays a role in formation of mature vacuoles and in
polarized cell growth"
/codon_start=1
/product="Opt2p"
/protein_id="NP_015520.1"
/db_xref="GeneID:856324"
/db_xref="SGD:S000006398"
/translation="MSETVKDKVIIDEKVSTKGTVDYAEGAEYSERLSNHSSDFSQWY
TDEQILHFMKKLGYENRTLYDIPEDVAYILKKMPELTLEDSFKILKDSIIYFKDDENI
PHDQYEEWKRLVDLEDLDSKEGIDEYDSFDIRAFASAIKFHSPYQEVRAVVDPEDDPT
IPVETFRAYFLAIIWSVIGSGFNEFFSHRVVSISLNTPIIQMFLYICGKAWAKTIPCW
TITIRGRKYGINIDKPWTQKEQMFSTLLYAICQGAFYTHYNILTQKLFYHSAFSFGYQ
FLLSLSVQFIGFGFAGILRKFVVYPARALWPTVMPTIAINKALLGKEKHESGMSRYKF
FFLTFFIMFIYNWFPTYIINILNTFNWMTWIKPSNINLANITGGVTGLGINPISSFDW
NVISFNSPLVYPFWSYLTQYLGCILAALIVIAVYYSNYMSCQYLPIFTNSLYTNTGHS
FKVTEVLDSDNKLDVKKYQSYSPPYYSAGNLVSYGAFICAYPLMITWSFIVHSKLLFN
AFKDWALNLWAMRKLKSWVTMFKSDYRALDDYDDPHSNAMKNYKEVPDWWYFAILIGS
LVVGIAVVEHYPTNTPVWGLFVCLGFNFVFLIPTTILQATTGYSFGLNLLIEMVMGYA
LPGNPIAIMILKAFGYNIDGQADNYVSNLKIAHYCKIPPMALFRGQCVIVFIQIFVNL
GVLNWQISNIKDFCTPHQNAKFTCPDAVTYYNASVVWGAIGPKRIFNYIYPIFKWCWL
IGACIGIFFGVWKRWGKFYPRYFDPMLFVGGMLNMSPPYNLMYYTSGMIVSYISQYYM
KRHHLNLWEKYNYVLSAGFSTGLVLSAIIIFFAVQYKDTAFNWWGNTVPYAGADGVGY
PLKNITDTANGYFGYAPGHYP"
gene complement(<927965..>928294)
/locus_tag="YPR195C"
/db_xref="GeneID:856325"
mRNA complement(<927965..>928294)
/locus_tag="YPR195C"
/product="uncharacterized protein"
/transcript_id="NM_001348889.1"
/db_xref="GeneID:856325"
CDS complement(927965..928294)
/locus_tag="YPR195C"
/note="hypothetical protein; conserved across S.
cerevisiae strains"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335827.1"
/db_xref="GeneID:856325"
/db_xref="SGD:S000006399"
/translation="MNSLIPLLVEASTYIVRGESSISIAIGIGPQASRSVPYHILCRG
CDGTVTTFRTWHTQPLGPCNTIIIGRKGNETTGGAEQRRQQHLTSDSATKASLVGFCG
LYYYFRK"
gene <931376..>932788
/locus_tag="YPR196W"
/db_xref="GeneID:856326"
mRNA <931376..>932788
/locus_tag="YPR196W"
/product="putative maltose-responsive transcription
factor"
/transcript_id="NM_001184293.1"
/db_xref="GeneID:856326"
CDS 931376..932788
/locus_tag="YPR196W"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:19111667]"
/note="Putative maltose-responsive transcription factor"
/codon_start=1
/product="putative maltose-responsive transcription
factor"
/protein_id="NP_015522.1"
/db_xref="GeneID:856326"
/db_xref="SGD:S000006400"
/translation="MSIVRQSCDCCRVRRVKCDRNRPCDRCRQRNLRCTYLQPLRKRG
PKSIGESNLERAAEIQMVTVNNNIMAAPVMYKKVPKKVIDQCLRLYHDQLYVIWPMLS
YDDLYKLLEENYEDCSTYWFLVSLSAATLSDLHTKIEYKKGFFFAGEQLCNLCMSSRR
FFDDLSNSDIFRIMTYYCLHRCYAQFADTRTSYRLSCEAIGLIKIAGFHREETYEFLP
FGEQQLIRKVYYLLLMTERYYAVYIKCVTSLDTTISPPQPEIVTDSRLSLDSFLEVIK
VFTVPGKYFYDALATNSVNGSYTEDSLKRIWNELHISSLDIEPYSYGYIDYLFSRHWV
RTLAWKLVLNKKDMRMNFFSNTNATHIPVEIAKDMLQDTLLTPIDLYDVHGPVIPMKA
LEIANALVDVVSKYDHNMKLEAWNILCDVSKFVFSLKHCNHKMFQRFSTKCQSALIDL
PISRPLRLNDDSKDEVDIIP"
rep_origin 932983..933229
/note="ARS1631; Autonomously Replicating Sequence"
/db_xref="SGD:S000118426"
repeat_region 933071..933404
/note="Ty4 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007219"
gene <934034..>935665
/gene="SGE1"
/locus_tag="YPR198W"
/gene_synonym="NOR1"
/db_xref="GeneID:856327"
mRNA <934034..>935665
/gene="SGE1"
/locus_tag="YPR198W"
/gene_synonym="NOR1"
/product="Sge1p"
/transcript_id="NM_001184295.1"
/db_xref="GeneID:856327"
CDS 934034..935665
/gene="SGE1"
/locus_tag="YPR198W"
/gene_synonym="NOR1"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:9483795]"
/experiment="EXISTENCE:mutant phenotype:GO:0042910
xenobiotic transmembrane transporter activity
[PMID:9483795]"
/experiment="EXISTENCE:mutant phenotype:GO:0055085
transmembrane transport [PMID:9483795]"
/note="Plasma membrane multidrug transporter; member of
the major facilitator superfamily; acts as an extrusion
permease; partial multicopy suppressor of gal11 mutations"
/codon_start=1
/product="Sge1p"
/protein_id="NP_015524.1"
/db_xref="GeneID:856327"
/db_xref="SGD:S000006402"
/translation="MKSTLSLTLCVISLLLTLFLAALDIVIVVTLYDTIGIKFHDFGN
IGWLVTGYALSNAVFMLLWGRLAEILGTKECLMISVIVFEIGSLISALSNSMATLISG
RVVAGFGGSGIESLAFVVGTSIVRENHRGIMITALAISYVIAEGVGPFIGGAFNEHLS
WRWCFYINLPIGAFAFIILAFCNTSGEPHQKMWLPSKIKKIMNYDYGELLKASFWKNT
FEVLVFKLDMVGIILSSAGFTLLMLGLSFGGNNFPWNSGIIICFFTVGPILLLLFCAY
DFHFLSLSGLHYDNKRIKPLLTWNIASNCGIFTSSITGFLSCFAYELQSAYLVQLYQL
VFKKKPTLASIHLWELSIPAMIATMAIAYLNSKYGIIKPAIVFGVLCGIVGSGLFTLI
NGELSQSIGYSILPGIAFGSIFQATLLSSQVQITSDDPDFQNKFIEVTAFNSFAKSLG
FAFGGNMGAMIFTASLKNQMRSSQLNIPQFTSVETLLAYSTEHYDGPQSSLSKFINTA
IHDVFYCALGCYALSFFFGIFTSSKKTTISAKKQQ"
gene complement(<938148..>939032)
/gene="ARR1"
/locus_tag="YPR199C"
/gene_synonym="ACR1; YAP8"
/db_xref="GeneID:856329"
mRNA complement(<938148..>939032)
/gene="ARR1"
/locus_tag="YPR199C"
/gene_synonym="ACR1; YAP8"
/product="Arr1p"
/transcript_id="NM_001184296.1"
/db_xref="GeneID:856329"
CDS complement(938148..939032)
/gene="ARR1"
/locus_tag="YPR199C"
/gene_synonym="ACR1; YAP8"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:18593383]"
/experiment="EXISTENCE:direct assay:GO:0001228 DNA-binding
transcription activator activity, RNA polymerase
II-specific [PMID:18593383]"
/experiment="EXISTENCE:direct assay:GO:0003677 DNA binding
[PMID:24358276]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:15147884|PMID:14978214]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:15147884]"
/experiment="EXISTENCE:genetic interaction:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:14978214|PMID:11527213]"
/experiment="EXISTENCE:genetic interaction:GO:0071243
cellular response to arsenic-containing substance
[PMID:14978214|PMID:11527213]"
/experiment="EXISTENCE:mutant phenotype:GO:0001228
DNA-binding transcription activator activity, RNA
polymerase II-specific [PMID:18593383]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:14978214|PMID:18593383|PMID:11527213|PMID:15147884|P
MID:15575969]"
/experiment="EXISTENCE:mutant phenotype:GO:0071243
cellular response to arsenic-containing substance
[PMID:18593383|PMID:11527213|PMID:15147884|PMID:15575969]"
/note="Transcriptional activator of the basic leucine
zipper (bZIP) family; required for transcription of genes
involved in resistance to arsenic compounds; directly
binds trivalent arsenic (As(III)) as does K. lactis
ortholog, KIYAP8"
/codon_start=1
/product="Arr1p"
/protein_id="NP_015525.1"
/db_xref="GeneID:856329"
/db_xref="SGD:S000006403"
/translation="MAKPRGRKGGRKPSLTPPKNKRAAQLRASQNAFRKRKLERLEEL
EKKEAQLTVTNDQIHILKKENELLHFMLRSLLTERNMPSDERNISKACCEEKPPTCNT
LDGSVVLSSTYNSLEIQQCYVFFKQLLSVCVGKNCTVPSPLNSFDRSFYPIGCTNLSN
DIPGYSFLNDAMSEIHTFGDFNGELDSTFLEFSGTEIKEPNNFITENTNAIETAAASM
VIRQGFHPRQYYTVDAFGGDVLLSAMDIWSFMKVHPKVNTFDLEILGTELKKSATCSN
FDILISLKHFIKVFSSKL"
gene complement(<939279..>939671)
/gene="ARR2"
/locus_tag="YPR200C"
/gene_synonym="ACR2"
/db_xref="GeneID:856330"
mRNA complement(<939279..>939671)
/gene="ARR2"
/locus_tag="YPR200C"
/gene_synonym="ACR2"
/product="Arr2p"
/transcript_id="NM_001184297.1"
/db_xref="GeneID:856330"
CDS complement(939279..939671)
/gene="ARR2"
/locus_tag="YPR200C"
/gene_synonym="ACR2"
/experiment="EXISTENCE:direct assay:GO:0030611 arsenate
reductase activity [PMID:10801893]"
/experiment="EXISTENCE:mutant phenotype:GO:0030611
arsenate reductase activity [PMID:10801893]"
/note="Arsenate reductase required for arsenate
resistance; converts arsenate to arsenite which can then
be exported from cells by Arr3p"
/codon_start=1
/product="Arr2p"
/protein_id="NP_015526.1"
/db_xref="GeneID:856330"
/db_xref="SGD:S000006404"
/translation="MVSFITSRQLKGLIENQRKDFQVVDLRREDFARDHITNAWHVPV
TAQITEKQLNQLIKGLSDTFSSSQFVKVIFHCTGSKNRGPKVAAKFETYLQEEDITSK
FESCILVGGFYAWETHCRESNLKLIVSG"
gene <939922..>941136
/gene="ARR3"
/locus_tag="YPR201W"
/gene_synonym="ACR3"
/db_xref="GeneID:856331"
mRNA <939922..>941136
/gene="ARR3"
/locus_tag="YPR201W"
/gene_synonym="ACR3"
/product="Arr3p"
/transcript_id="NM_001184298.1"
/db_xref="GeneID:856331"
CDS 939922..941136
/gene="ARR3"
/locus_tag="YPR201W"
/gene_synonym="ACR3"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:20655873]"
/experiment="EXISTENCE:direct assay:GO:0015105 arsenite
transmembrane transporter activity [PMID:24291645]"
/experiment="EXISTENCE:mutant phenotype:GO:0015104
antimonite transmembrane transporter activity
[PMID:20655873]"
/experiment="EXISTENCE:mutant phenotype:GO:0015105
arsenite transmembrane transporter activity
[PMID:24291645]"
/experiment="EXISTENCE:mutant phenotype:GO:0015297
antiporter activity [PMID:21447319]"
/experiment="EXISTENCE:mutant phenotype:GO:0015699
antimonite transmembrane transport [PMID:20655873]"
/experiment="EXISTENCE:mutant phenotype:GO:0015700
arsenite transport [PMID:24291645]"
/note="Plasma membrane metalloid/H+ antiporter; member of
bile/arsenite/riboflavin transporter (BART) superfamily;
transports arsenite and antimonite; required for
resistance to arsenic compounds; transcription is
activated by Arr1p in the presence of arsenite; protein
displays 10 transmembrane segments with cytoplasmically
oriented N- and C-terminal domains"
/codon_start=1
/product="Arr3p"
/protein_id="NP_015527.1"
/db_xref="GeneID:856331"
/db_xref="SGD:S000006405"
/translation="MSEDQKSENSVPSKVNMVNRTDILTTIKSLSWLDLMLPFTIILS
IIIAVIISVYVPSSRHTFDAEGHPNLMGVSIPLTVGMIVMMIPPICKVSWESIHKYFY
RSYIRKQLALSLFLNWVIGPLLMTALAWMALFDYKEYRQGIIMIGVARCIAMVLIWNQ
IAGGDNDLCVVLVITNSLLQMVLYAPLQIFYCYVISHDHLNTSNRVLFEEVAKSVGVF
LGIPLGIGIIIRLGSLTIAGKSNYEKYILRFISPWAMIGFHYTLFVIFISRGYQFIHE
IGSAILCFVPLVLYFFIAWFLTFALMRYLSISRSDTQRECSCDQELLLKRVWGRKSCE
ASFSITMTQCFTMASNNFELSLAIAISLYGNNSKQAIAATFGPLLEVPILLILAIVAR
ILKPYYIWNNRN"
rep_origin 942084..943032
/note="ARS1632; Putative replication origin; identified in
multiple array studies, not yet confirmed by plasmid-based
assay"
/db_xref="SGD:S000130202"
telomere 942396..948010
/note="TEL16R; Telomeric region on the right arm of
Chromosome XVI; annotated components include an X element
core sequence and a short Y' element; TEL16R does have
telomeric repeats (TEL16R-TR), but they are missing from
the genome annotation due to difficulties encountered
during sequencing and/or assembly"
/db_xref="SGD:S000029002"
gene <943032..>943896
/locus_tag="YPR202W"
/db_xref="GeneID:856332"
mRNA join(<943032..943050,943199..>943896)
/locus_tag="YPR202W"
/product="uncharacterized protein"
/transcript_id="NM_001184299.1"
/db_xref="GeneID:856332"
CDS join(943032..943050,943199..943896)
/locus_tag="YPR202W"
/note="hypothetical protein; similar to telomere-encoded
helicases; down-regulated at low calcium levels; YPR202W
is not an essential gene; transcript is predicted to be
spliced but there is no evidence that it is spliced in
vivo"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015528.1"
/db_xref="GeneID:856332"
/db_xref="SGD:S000006406"
/translation="MEIENEQICTCIAQILHLLNSLIITFSDDDKTETGQSFVYIDGF
LVKKHNNQHTIVNFETYKNKMKVSDRRKFEKANFDEFESALNNKNDLVHCPSITLFES
IPTEVRSFYEDEKSGLIKVVKFRTGAMDRKRSFEKIVISVMVGKNVQKFLTFVEDEPD
FQGGPIPSKYLIPKKINLMVYTLFQVHTLKFNRKDYDTLSLFYLNRGYYNELSFPCPG
TLSRNSECQAERQLYDAYFH"
gene <943880..>944188
/locus_tag="YPR203W"
/db_xref="GeneID:856333"
mRNA <943880..>944188
/locus_tag="YPR203W"
/product="uncharacterized protein"
/transcript_id="NM_001184300.1"
/db_xref="GeneID:856333"
CDS 943880..944188
/locus_tag="YPR203W"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_015529.1"
/db_xref="GeneID:856333"
/db_xref="SGD:S000006407"
/translation="MRTFTDFVSGAPIVRSLQKSTIRKYGYNLAPHMFLLLHVDELSI
FSAYQASLPGEKKVDTERLKRDLCPRKPIEIKYFSQICNDMMNKKDRLGDVLHVCCPS
"
gene <944603..>947701
/locus_tag="YPR204W"
/db_xref="GeneID:856334"
mRNA <944603..>947701
/locus_tag="YPR204W"
/product="Y' element ATP-dependent helicase"
/transcript_id="NM_001184301.1"
/db_xref="GeneID:856334"
CDS 944603..947701
/locus_tag="YPR204W"
/experiment="EXISTENCE:direct assay:GO:0003678 DNA
helicase activity [PMID:9837911]"
/note="DNA helicase encoded within the telomeric Y'
element; Y' -helicase protein 1"
/codon_start=1
/product="Y' element ATP-dependent helicase"
/protein_id="NP_015530.1"
/db_xref="GeneID:856334"
/db_xref="SGD:S000006408"
/translation="MADTPSVAVQAPPGYGKTELFHLPLIALASKGDVKYVSFLFVPY
TVLLANCMIRLGRCGCLNVAPVRNFIEEGCDGVTDLYVGIYDDLASTNFTDRIAAWEN
IVECTFRTNNVKLGYLIVDEFHNFETEVYRQSQFGGITNLDFDAFEKAIFLSGTAPEA
VADAALQRIGLTGLAKKSMDINELKRSEDLSRGLSSYPTRMFNLIKEKSEVPLGHVHK
IWKKVESQPEEALKLLLALFEIEPESKAIVVASTTNEVEELACSWRKYFRVVWIHGKL
GAAEKVSRTKEFVTDGSMRVLIGTKLVTEGIDIKQLMMVIMLDNRLNIIELIQGVGRL
RDGGLCYLLSRKNSWAARNRKGELPPIKEGCITEQVREFYGLESKKGKKGQHVGCCGS
RTDLSADTVELIERMDRLAEKQATASMSIVALPSSFQESNSSDRCRKYCSSDEDSNTC
IHGSANASTNATTNSSTNATTTASTNVRTSATTTASINVRTSATTTESTNSSTNATTT
ASTNVRTSATTTASINVRTSATTTESTNSNTSATTTESTDSNTSATTTESTNSSTNAT
TTASINVRTSATTTESTNSNTNATTTESTNSSTNATTTEGTNSNTSATTTASTNSSTN
ATTTESTNASAKEDANKDGNAEDNRFHPVTDINKESYKRKGSQMVLLERKKLKAQFPN
TSENMNVLQFLGFRSDEIKHLFLYGIDVYFCPEGVFTQYGLCKGCQKMFELCVCWAGQ
KVSYRRMAWEALAVERMLRNDEEYKEYLEDIEPYHGDPVGYLKYFSVKRGEIYSQIQR
NYAWYLAITRRRETISVLDSTRGKQGSQVFRMSGRQIKELYYKVWSNLRESKTEVLQY
FLNWDEKKCREEWEAKDDTVFVEALEKVGVFQRLRSMTSAGLQGPQYVKLQFSRHHRQ
LRSRYELSLGMHLRDQLALGVTPSKVPHWTAFLSMLIGLFCNKTFRQKLEYLLEQISE
VWLLPHWLDLANVEVLAADNTRVPLYMLMVAVHKELDSDDVPDGRFDILLCRDSSREV
GE"
CONTIG join(BK006949.2:1..948066)
//
