Contains FAD as prosthetic group. One of several enzymes that catalyse the first step in fatty acids beta-oxidation. The enzyme from pig liver can accept substrates with acyl chain lengths of 4 to 16 carbon atoms, but is most active with C8 to C12 compounds [2]. The enzyme from rat does not accept C16 at all and is most active with C6-C8 compounds [4]. cf. EC
1.3.8.1, short-chain acyl-CoA dehydrogenase, EC
1.3.8.8, long-chain acyl-CoA dehydrogenase, and EC
1.3.8.9, very-long-chain acyl-CoA dehydrogenase.