LOCUS NC_001142 745751 bp DNA linear CON 23-JAN-2026
DEFINITION Saccharomyces cerevisiae S288C chromosome X, complete sequence.
ACCESSION NC_001142
VERSION NC_001142.9
DBLINK BioProject: PRJNA128
Assembly: GCF_000146045.2
KEYWORDS RefSeq.
SOURCE Saccharomyces cerevisiae S288C
ORGANISM Saccharomyces cerevisiae S288C
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
Saccharomyces.
REFERENCE 1 (bases 1 to 745751)
AUTHORS Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M.,
Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S.,
Weng,S. and Cherry,J.M.
TITLE New data and collaborations at the Saccharomyces Genome Database:
updated reference genome, alleles, and the Alliance of Genome
Resources
JOURNAL Genetics 220 (4) (2022)
PUBMED 34897464
REFERENCE 2 (bases 1 to 745751)
AUTHORS Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
Oliver,S.G.
TITLE Life with 6000 genes
JOURNAL Science 274 (5287), 546 (1996)
PUBMED 8849441
REFERENCE 3 (bases 1 to 745751)
AUTHORS Galibert,F., Alexandraki,D., Baur,A., Boles,E., Chalwatzis,N.,
Chuat,J.C., Coster,F., Cziepluch,C., De Haan,M., Domdey,H.,
Durand,P., Entian,K.D., Gatius,M., Goffeau,A., Grivell,L.A.,
Hennemann,A., Herbert,C.J., Heumann,K., Hilger,F., Hollenberg,C.P.,
Huang,M.E., Jacq,C., Jauniaux,J.C., Katsoulou,C.,
Karpfinger-Hartl,L. et al.
TITLE Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
X
JOURNAL EMBO J. 15 (9), 2031-2049 (1996)
PUBMED 8641269
REFERENCE 4 (bases 1 to 745751)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (23-JAN-2026) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 5 (bases 1 to 745751)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (04-MAY-2012) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Protein update by submitter
REFERENCE 6 (bases 1 to 745751)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (12-APR-2011) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Sequence update by submitter
REFERENCE 7 (bases 1 to 745751)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (14-DEC-2009) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
COMMENT REVIEWED REFSEQ: This record has been curated by SGD. The reference
sequence is identical to BK006943.
On Apr 26, 2011 this sequence version replaced NC_001142.8.
##Genome-Annotation-Data-START##
Annotation Provider :: SGD
Annotation Status :: Full Annotation
Annotation Version :: R64-4-1
URL :: http://www.yeastgenome.org/
##Genome-Annotation-Data-END##
COMPLETENESS: full length.
FEATURES Location/Qualifiers
source 1..745751
/organism="Saccharomyces cerevisiae S288C"
/mol_type="genomic DNA"
/strain="S288C"
/db_xref="taxon:559292"
/chromosome="X"
telomere complement(1..7767)
/note="TEL10L; Telomeric region on the left arm of
Chromosome X; composed of an X element core sequence, X
element combinatorial repeats, a long Y' element, and a
short terminal stretch of telomeric repeats"
/db_xref="SGD:S000028901"
rep_origin 65..577
/note="ARS1001; Autonomously Replicating Sequence"
/db_xref="SGD:S000028438"
gene complement(<466..>6130)
/locus_tag="YJL225C"
/db_xref="GeneID:853231"
mRNA complement(join(<466..4581,4970..>6130))
/locus_tag="YJL225C"
/product="Y' element ATP-dependent helicase"
/transcript_id="NM_001181657.1"
/db_xref="GeneID:853231"
CDS complement(join(466..4581,4970..6130))
/locus_tag="YJL225C"
/note="Putative Y' element ATP-dependent helicase"
/codon_start=1
/product="Y' element ATP-dependent helicase"
/protein_id="NP_012311.1"
/db_xref="GeneID:853231"
/db_xref="SGD:S000003760"
/translation="MKVSDRRKFEKANFDEFESALNNKNDLVHCPSITLFESIPTEVR
SFYEDEKSGLIKVVKFRTGAMDRKRSFEKVVISVMVGKNVKKFLTFVEDEPDFQGGPI
PSKYLIPKKINLMVYTLFQVHTLKFNRKDYDTLSLFYLNRGYYNELSFRVLERCHEIA
SARPNDSSTMRTFTDFVSGAPIVRSLQKSTIRKYGYNLAPYMFLLLHVDELSIFSAYQ
ASLPGEKKVDTERLKRDLCPRKPIEIKYFSQICNDMMNKKDRLGDILHIILRACALNF
GAGPRGGAGDEEDRSITNEEPIIPSVDEHGLKVCKLRSPNTPRRLRKTLDAVKALLVS
SCACTARDLDIFDDNNGVAMWKWIKILYHEVAQETTLKDSYRITLVPSSDGISLLAFA
GPQRNVYVDDTTRRIQLYTDYNKNGSSEPRLKTLDGLTSDYVFYFVTVLRQMQICALG
NSYDAFNHDPWMDVVGFEDPNQVTNRDISRIVLYSYMFLNTAKGCLVEYATFRQYMRE
LPKNAPQKLNFREMRQGLIALGRHCVGSRFETDLYESATSELMANHSVQTGRNIYGVD
SFSLTSVSGTTATLLQERASERWIQWLGLESDYHCSFSSTRNAEDVVAGEAASSNHHQ
KISRVTRKRPREPKSTNDILVAGQKLFGSSFEFRDLHQLRLCYEIYMADTPSVAVQAP
PGYGKTELFHLPLIALASKGDVEYVSFLFVPYTVLLANCMIRLGRRGCLNVAPVRNFI
EEGYDGVTDLYVGIYDDLASTNFTDRIAAWENIVECTFRTNNVKLGYLIVDEFHNFET
EVYRQSQFGGITNLDFDAFEKAIFLSGTAPEAVADAALQRIGLTGLAKKSMDINELKR
SEDLSRGLSSYPTRMFNLIKEKSEVPLGHVHKIRKKVESQPEEALKLLLALFESEPES
KAIVVASTTNEVEELACSWRKYFRVVWIHGKLGAAEKVSRTKEFVTDGSMQVLIGTKL
VTEGIDIKQLMMVIMLDNRLNIIELIQGVGRLRDGGLCYLLSRKNSWAARNRKGELPP
IKEGCITEQVREFYGLESKKGKKGQHVGCCGSRTDLSADTVELIERMDRLAEKQATAS
MSIVALPSSFQESNSSDRYRKYCSSDEDSNTCIHGSANASTNASTNAITTASTNVRTN
ATTNASTNATTNASTNASTNATTNASTNATTNSSTNATTTASTNVRTSATTTASINVR
TSATTTESTNSSTNATTTESTNSSTNATTTESTNSNTSATTTASINVRTSATTTESTN
SSTSATTTASINVRTSATTTKSINSSTNATTTESTNSNTNATTTESTNSSTNATTTES
TNSSTNATTTESTNSNTSAATTESTNSNTSATTTESTNASAKEDANKDGNAEDNRFHP
VTDINKESYKRKGSQMVLLERKKLKAQFPNTSENMNVLQFLGFRSDEIKHLFLYGIDI
YFCPEGVFTQYGLCKGCQKMFELCVCWAGQKVSYRRIAWEALAVERMLRNDEEYKEYL
EDIEPYHGDPVGYLKYFSVKRREIYSQIQRNYAWYLAITRRRETISVLDSTRGKQGSQ
VFRMSGRQIKELYFKVWSNLRESKTEVLQYFLNWDEKKCQEEWEAKDDTVVVEALEKG
GVFQRLRSMTSAGLQGPQYVKLQFSRHHRQLRSRYELSLGMHLRDQIALGVTPSKVPH
WTAFLSMLIGLFYNKTFRQKLEYLLEQISEVWLLPHWLDLANVEVLAADDTRVPLYML
MVAVHKELDSDDVPDGRFDILLCRDSSREVGE"
rep_origin 7445..7943
/note="ARS1002; Autonomously Replicating Sequence"
/db_xref="SGD:S000028439"
gene complement(<8776..>9138)
/gene="PAU1"
/locus_tag="YJL223C"
/db_xref="GeneID:853232"
mRNA complement(<8776..>9138)
/gene="PAU1"
/locus_tag="YJL223C"
/product="seripauperin PAU1"
/transcript_id="NM_001181656.1"
/db_xref="GeneID:853232"
CDS complement(8776..9138)
/gene="PAU1"
/locus_tag="YJL223C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/note="Member of the seripauperin multigene family;
encoded mainly in subtelomeric regions; SWAT-GFP and
mCherry fusion proteins localize to the vacuole; active
during alcoholic fermentation; regulated by anaerobiosis,
negatively regulated by oxygen; repressed by heme;
identical to Pau14p"
/codon_start=1
/product="seripauperin PAU1"
/protein_id="NP_012312.1"
/db_xref="GeneID:853232"
/db_xref="SGD:S000003759"
/translation="MVKLTSIAAGVAAIAATASATTTLAQSDERVNLVELGVYVSDIR
AHLAQYYMFQAAHPTETYPVEVAEAVFNYGDFTTMLTGISPDQVTRMITGVPWYSSRL
KPAISSALSKDGIYTIAN"
gene <11475..>16124
/gene="VTH2"
/locus_tag="YJL222W"
/db_xref="GeneID:853233"
mRNA <11475..>16124
/gene="VTH2"
/locus_tag="YJL222W"
/product="signal sequence-binding protein"
/transcript_id="NM_001181655.1"
/db_xref="GeneID:853233"
CDS 11475..16124
/gene="VTH2"
/locus_tag="YJL222W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0005048
signal sequence binding [PMID:8636229]"
/experiment="EXISTENCE:genetic interaction:GO:0006623
protein targeting to vacuole [PMID:8636229]"
/experiment="EXISTENCE:genetic interaction:GO:0006896
Golgi to vacuole transport [PMID:8662642]"
/note="Putative membrane glycoprotein; has strong
similarity to Vth1p and Pep1p/Vps10p; may be involved in
vacuolar protein sorting"
/codon_start=1
/product="signal sequence-binding protein"
/protein_id="NP_012313.1"
/db_xref="GeneID:853233"
/db_xref="SGD:S000003758"
/translation="MALFRALYIIWVFLLIPLSNAEEFTPKVTRTLSRYVFDIVNFDD
SNTLIRAEEDSVEISFDAGENWKTIDEIEEPIESFVVDPFRGHDRAFAFVKTAPKFYV
TDDQGKSWRPLTIPISEKASNYFCDVTTHPIKKKHLIIRCDLLTIKNSGLMYVGREIY
TTNDGVSFSQVKPSFGKIDGHISTARCDFIKSSEDSDLGGNDASILCLFRNTEYIEST
GSTIDKSELILSADGGETFKELVQFKDKVVSRYEILKHHVIVLTQDDMYNEMSSTNIW
ISNDVSTFQVARTPTKIRHVNMGQIHEDSIGRIVLPVSRERDDEDSNQPGAAEVLISD
SEGLKFLPINWIPNNQFGYINVAYPGFLKGTFFGSFHPFIEYSDRKRKYSRQKVREET
KVSVDNGLTWTNLKVVDRENVDLFGCDVTKPERCSLQTHFYDLRNLNPSAGIMMISGI
VGDGSAYNWKEEKTFISRDSGLTWRLVHNSTGLYTTGDLGNIIMYIPYRSNENGDVPS
KFYYSLDQGKTWGEYDLIMPIYPYRLVSTISDGSGSKFILTGTSITEDPIFITYSIDF
SAVFDYKSCEEGDFEDWNLADGKCVNGAKYKYRRRKQDAQCLVKKAFKDLSLDETPCN
SCTGSDYECSFEFVRDAKGDCIPDYNLIALSDICDKSKGKSVLVKPLQLIKGDKCKTP
MKIESVDIPCDEIPKEGSSDKEIVTTENKFDFEIKFYQYFDTVADESLVMLNSIGDAY
ISHDGGQTIKRFDTDGEKIVEIVFNPYFNSSAYLFGSKGNIFLTHDRGYSFMIAKLPE
ARQLGMPLDFSAKAQDTFIYYGGKNCESILSPECHAVAYLTKDGGETFTEMLDNAIHC
EFAGTLFKYPSNDDMVMCQVKEKFSQTRSLVSSTDFFQDDRKTVFENIIGYLSTGGYI
IVAVPHEDNELRAYVTNDGAEFTEAKFPYDEDIGKQDAFTILGSEEGSIFLHLATNLE
SGHDFGNLLKSNSNGTSFVTLEHAVNRNTFGYVDFEKVQGLEGIIITNIVSNSEKVGE
NKEDEQLKTKITFNDGSDWNFLKPPKKDSEGKKFPCDSVSLDKCSLHLHGYTERKDIR
DTYSSGSALGMMFGVGNVGDRLLPYEECSTFLTTDGGETWTEVKKGPHQWEYGDHGGV
LVLVPENAETDSISYSTDFGKTWKDYKFCGDKVLVKDIITVPRDSALRFLLFGEAKNM
GSGSFRTYTIDFRNIFERQCEFDITGRKRADFKYSPLGSRTGCLFGHKTEFLRKTDEK
CFIGNIPLSEFSRNVKNCPCTRQDFECDYNFYKASDGTCKLVKGLSSANGADICKKEP
DLIEYYDSSGYRKIPLSTCKGGLKLDAHLAPHPCPGKEKAFREKYSINTGAYALVFVT
ILLVIFFVAWFVYDRGIRRNGGFSRFEEIRLGDDGLIENNRTDRVVNIIVRLGLCISL
ITKSAFQRAKAGTAQLSSKFRARFGNKKGATYSSLLHDQLSDEPDGFHEDSNDLSSFR
GQGSNSEIEQEDVDTSQQEHTSRTDLLGASNIPDALPARSASHESDLAAARSEDK"
rep_origin 16124..16762
/note="ARS1003; Autonomously Replicating Sequence"
/db_xref="SGD:S000028440"
gene complement(<16767..>18536)
/gene="IMA4"
/locus_tag="YJL221C"
/gene_synonym="FSP2"
/db_xref="GeneID:853235"
mRNA complement(<16767..>18536)
/gene="IMA4"
/locus_tag="YJL221C"
/gene_synonym="FSP2"
/product="oligo-1,6-glucosidase IMA4"
/transcript_id="NM_001181654.1"
/db_xref="GeneID:853235"
CDS complement(16767..18536)
/gene="IMA4"
/locus_tag="YJL221C"
/gene_synonym="FSP2"
/EC_number="3.2.1.10"
/experiment="EXISTENCE:genetic interaction:GO:0046352
disaccharide catabolic process [PMID:20562106]"
/experiment="EXISTENCE:mutant phenotype:GO:0004574
oligo-1,6-glucosidase activity [PMID:20562106]"
/note="Alpha-glucosidase; weak, but broad substrate
specificity for alpha-1,4- and alpha-1,6-glucosides;
member of IMA isomaltase family; not required for
isomaltose utilization, but Ima4p overexpression allows
the ima1 null mutant to grow on isomaltose; identical to
IMA3"
/codon_start=1
/product="oligo-1,6-glucosidase IMA4"
/protein_id="NP_012314.1"
/db_xref="GeneID:853235"
/db_xref="SGD:S000003757"
/translation="MTISSAHPETEPKWWKEATIYQIYPASFKDSNNDGWGDMKGIAS
KLEYIKELGTDAIWISPFYDSPQDDMGYDIANYEKVWPTYGTNEDCFALIEKTHKLGM
KFITDLVINHCSSEHEWFKESRSSKTNPKRDWFFWRPPKGYDAEGKPIPPNNWRSYFG
GSAWTFDEKTQEFYLRLFCSTQPDLNWENEDCRKAIYESAVGYWLDHGVDGFRIDVGS
LYSKVAGLPDAPVIDENSKWQLSDPFTMNGPRIHEFHQEMNKFIRNRVKDGREIMTVG
EMRHATDETKRLYTSASRHELSELFNFSHTDVGTSPKFRQNLIPYELKDWKVALAELF
RYVNGTDCWSTIYLENHDQPRSITRFGDDSPKNRVISGKLLSVLLVSLSGTLYVYQGQ
ELGEINFKNWPIEKYEDVEVRNNYDAIKEEHGENSKEMKRFLEAIALISRDHARTPMQ
WSREEPNAGFSGPNAKPWFYLNESFREGINAEDESKDPNSVLNFWKEALRFRKAHKDI
TVYGYDFEFIDLDNKKLFSFTKKYDNKTLFAALNFSSDSIDFTIPNNSSSFKLEFGNY
PRSEVDASSRTLKPWEGRIYISE"
gene <19497..>21200
/gene="HXT9"
/locus_tag="YJL219W"
/db_xref="GeneID:853236"
mRNA <19497..>21200
/gene="HXT9"
/locus_tag="YJL219W"
/product="hexose transporter HXT9"
/transcript_id="NM_001181652.1"
/db_xref="GeneID:853236"
CDS 19497..21200
/gene="HXT9"
/locus_tag="YJL219W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0005353
fructose transmembrane transporter activity
[PMID:10618490]"
/experiment="EXISTENCE:mutant phenotype:GO:0005354
galactose transmembrane transporter activity
[PMID:10618490]"
/experiment="EXISTENCE:mutant phenotype:GO:0008645 hexose
transmembrane transport [PMID:10618490]"
/experiment="EXISTENCE:mutant phenotype:GO:0015578 mannose
transmembrane transporter activity [PMID:10618490]"
/experiment="EXISTENCE:mutant phenotype:GO:0055056
D-glucose transmembrane transporter activity
[PMID:10618490]"
/note="Putative hexose transporter that is nearly
identical to Hxt11p; has similarity to major facilitator
superfamily (MFS) transporters, expression of HXT9 is
regulated by transcription factors Pdr1p and Pdr3p"
/codon_start=1
/product="hexose transporter HXT9"
/protein_id="NP_012316.1"
/db_xref="GeneID:853236"
/db_xref="SGD:S000003755"
/translation="MSGVNNTSANDLSTTESNSNSVANAPSVKTEHNDSKNSLNLDAT
EPPIDLPQKPLSAYTTVAILCLMIAFGGFIFGWDTGTISGFVNLSDFIRRFGQKNDKG
TYYLSKVRMGLIVSIFNIGCAIGGIVLSKVGDIYGRRIGLITVTAIYVVGILIQITSI
NKWYQYFIGRIISGLGVGGIAVLSPMLISEVAPKQIRGTLVQLYQLMCTMGIFLGYCT
NYGTKNYHNATQWRVGLGLCFAWTTFMVSGMMFVPESPRYLIEVGKDEEAKRSLSKSN
KVSVDDPALLAEYDTIKAGIELEKLAGNASWSELLSTKTKVFQRVLMGVMIQSLQQLT
GDNYFFYYGTTIFKSVGLKDSFQTSIIIGVVNFFSSFIAVYTIERFGRRTCLLWGAAS
MLCCFAVFASVGVTKLWPQGSSHQDITSQGAGNCMIVFTMFFIFSFATTWAGGCYVIV
SETFPLRVKSRGMAIATAANWMWGFLISFFTPFITGAINFYYGYVFLGCLVFAYFYVF
FFVPETKGLTLEEVNTMWLEGVPAWKSASWVPPERRTADYDADAIDHDDRPIYKRFFS
S"
gene <21973..>22563
/locus_tag="YJL218W"
/db_xref="GeneID:853237"
mRNA <21973..>22563
/locus_tag="YJL218W"
/product="acetyltransferase"
/transcript_id="NM_001181651.1"
/db_xref="GeneID:853237"
CDS 21973..22563
/locus_tag="YJL218W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:31945465]"
/note="Mitochondrial protein, putative acetyltransferase;
similar to bacterial galactoside O-acetyltransferases;
induced by oleate in OAF1/PIP2-dependent manner; promoter
contains oleate response element consensus sequence;
mutation decreases salt tolerance; non-essential gene"
/codon_start=1
/product="acetyltransferase"
/protein_id="NP_012317.1"
/db_xref="GeneID:853237"
/db_xref="SGD:S000003754"
/translation="MGVLENIVPGELYDANYDPDLLKIRKETKIKLHEYNTLSPADEN
KKSQVIRELLGSCTDNFIIEPPFYCDYGSNIYIGDNFYANHNLVILDGAKVVIGDNVF
IAPNVGIYTAGHPIDVERRLQGLEYAMPVTIGDNVWIGGGVSIIPGVNIGKNSVIAAG
SVVIRDIPENVVAAGNPCKVIRKITEKDSTTTNYRK"
gene <23133..>23729
/gene="REE1"
/locus_tag="YJL217W"
/db_xref="GeneID:853238"
mRNA <23133..>23729
/gene="REE1"
/locus_tag="YJL217W"
/product="Ree1p"
/transcript_id="NM_001181650.1"
/db_xref="GeneID:853238"
CDS 23133..23729
/gene="REE1"
/locus_tag="YJL217W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="Cytoplasmic protein involved in the regulation of
enolase (ENO1); mRNA expression is induced by calcium
shortage, copper deficiency (via Mac1p) and the presence
of galactose (via Gal4p); mRNA expression is also
regulated by the cell cycle"
/codon_start=1
/product="Ree1p"
/protein_id="NP_012318.1"
/db_xref="GeneID:853238"
/db_xref="SGD:S000003753"
/translation="MVESKNTELSQGTWLNKPKSVFQEAGKVTLETDEKTDFWRETFY
GFTRDSGHFLGVETGSAFTAQVRVQGSYESLYDQAGIMVRIDDGHWLKAGIEISDGHA
MLSSVLTNGKSDWSTAVYGGNARDFWLRVTVEKGVLRIQVSSDKKTWPLVRLAPFPTS
DHYLVGPMACTPERGGLKVTFSEWSLTAPLGKALHDLS"
rep_origin 23662..24158
/note="ARS1004; Autonomously Replicating Sequence"
/db_xref="SGD:S000028441"
gene complement(<24341..>26086)
/gene="IMA5"
/locus_tag="YJL216C"
/db_xref="GeneID:853214"
mRNA complement(<24341..>26086)
/gene="IMA5"
/locus_tag="YJL216C"
/product="oligo-1,6-glucosidase IMA5"
/transcript_id="NM_001181649.1"
/db_xref="GeneID:853214"
CDS complement(24341..26086)
/gene="IMA5"
/locus_tag="YJL216C"
/EC_number="3.2.1.10"
/experiment="EXISTENCE:direct assay:GO:0004574
oligo-1,6-glucosidase activity [PMID:20471265]"
/experiment="EXISTENCE:genetic interaction:GO:0046352
disaccharide catabolic process
[PMID:20471265|PMID:20562106]"
/experiment="EXISTENCE:mutant phenotype:GO:0004574
oligo-1,6-glucosidase activity [PMID:20562106]"
/note="Alpha-glucosidase; specificity for isomaltose,
maltose, and palatinose, but not alpha-methylglucoside;
most distant member of the IMA isomaltase family, but with
similar catalytic properties as Ima1p and Ima2p; not
required for isomaltose utilization, but Ima5p
overexpression allows the ima1 null mutant to grow on
isomaltose; can cleave alpha-1,3 linkage of nigerose and
turanose and alpha-1,5 linkage of leucrose and is very
sensitive to temperature in vitro"
/codon_start=1
/product="oligo-1,6-glucosidase IMA5"
/protein_id="NP_012319.1"
/db_xref="GeneID:853214"
/db_xref="SGD:S000003752"
/translation="MTIIHNPKWWKEATVYQIYPASFKDSNNDGWGDLAGITSKLDYV
KELGVDAIWVCPFYDSPQEDMGYDIANYEKVWPRYGTNEDCFQMIEEAHKRGIKVIVD
LVINHCSEEHEWFKESRSSKANPKRDWFFWRPPKGYDEKGNPIPPNNWRSFFGGSAWR
YDEKTGEFFLHVFALGQPDFNWENEECRKAIYDSSVGYWLRHNVDGFRIDVGSMYSKV
EGLPDAPITDPTVPYQKGTEFFINGPRIHEYHKEMHNYMLSQVPEGKEIMTVGEVGIG
NEDDFRVYTSAKEGELNMMFNFKHTSVGENPKCKYELIPFTLKDFKLALAESFLFIEN
TDCWSTIYLENHDQPRSVSRFGSDSPKWREISSKMLATLIISLTGTVFIYQGQELGMP
NFKNRKIEQIKCVEGTGTYAAIKRDYGEDSEKMKKFFEALALISRDHGRTPFPWSADE
PSAGFSKDAKPWIDMNESFRDGINAEAELKDKNSVFFFWKKALQVRKEHKDILVYGHN
FQFIDLDNDKLFMFTKDTDNKKMFAVFNFSSDNTDFSVPDNEASYTMFFGNYANSNGD
SRTLQPWEGRLYLLK"
gene <26887..>28596
/gene="HXT8"
/locus_tag="YJL214W"
/db_xref="GeneID:853216"
mRNA <26887..>28596
/gene="HXT8"
/locus_tag="YJL214W"
/product="hexose transporter HXT8"
/transcript_id="NM_001181647.1"
/db_xref="GeneID:853216"
CDS 26887..28596
/gene="HXT8"
/locus_tag="YJL214W"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0005353
fructose transmembrane transporter activity
[PMID:10618490]"
/experiment="EXISTENCE:mutant phenotype:GO:0008645 hexose
transmembrane transport [PMID:10618490]"
/experiment="EXISTENCE:mutant phenotype:GO:0015578 mannose
transmembrane transporter activity [PMID:10618490]"
/experiment="EXISTENCE:mutant phenotype:GO:0055056
D-glucose transmembrane transporter activity
[PMID:10618490]"
/note="hypothetical protein with similarity to hexose
transporters; expression is induced by low levels of
glucose and repressed by high levels of glucose"
/codon_start=1
/product="hexose transporter HXT8"
/protein_id="NP_012321.1"
/db_xref="GeneID:853216"
/db_xref="SGD:S000003750"
/translation="MTDRKTNLPEEPIFEEAEDDGCPSIENSSHLSVPTVEENKDFSE
YNGEEAEEVVVPEKPASAYATVSIMCLCMAFGGFMSGWDTGTISGFVNQTDFLRRFGN
YSHSKNTYYLSNVRTGLIVSIFNVGSAIGCLFLSKLGDIYGRCMGLIIVIVVYMVGIV
IQIASIDKWYQYFIGRIIAGIGAGSISVLAPMLISETAPKHIRGTLLACWQLMVTFAI
FLGYCTNYGTKTYSNSVQWRVPLGLCFAWAIIMIGGMTFVPESPRFLVQVGKIEQAKA
SFAKSNKLSVDDPAVVAEIDLLVAGVEAEEAMGTASWKELFSRKTKVFQRLTMTVMIN
SLQQLTGDNYFFYYGTTIFKSVGMNDSFETSIVLGIVNFASCFFSLYSVDKLGRRRCL
LLGAATMTACMVIYASVGVTRLYPNGKSEPSSKGAGNCTIVFTCFYIFCFSCTWGPVC
YVIISETFPLRVRSKCMSVATAANLLWGFLIGFFTPFITSAINFYYGYVFMGCLAFSY
FYVFFFVPETKGLTLEEVDEMWMDGVLPWKSESWVPASRRDGDYDNEKLQHDEKPFYK
RMF"
gene <32163..>33158
/locus_tag="YJL213W"
/db_xref="GeneID:853217"
mRNA <32163..>33158
/locus_tag="YJL213W"
/product="uncharacterized protein"
/transcript_id="NM_001181646.1"
/db_xref="GeneID:853217"
CDS 32163..33158
/locus_tag="YJL213W"
/note="hypothetical protein that may interact with
ribosomes; periodically expressed during the yeast
metabolic cycle; phosphorylated in vitro by the mitotic
exit network (MEN) kinase complex, Dbf2p/Mob1p"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_012322.1"
/db_xref="GeneID:853217"
/db_xref="SGD:S000003749"
/translation="MLMPKSVALLRIRYTLEAALARGFTTVRDCGGAEGFLKAEIRQG
SLNGPRLITCGHAISQTGGHGDLRSGALPASAFDSCSCHFGQVGVVADGVPECYKAAR
EEFRRGADFIKIMGGGGVASPTDKISNKQFCDDEIKALVDVANSYHTYVTAHAYTAEA
IQNCIKLGVKGIEHGNLLDERTAELMAELGCYLTPTLVTYKVMGSDQFSAFLGPENSR
KNTEVLYKGIDAMKIAQKKKVKICFGSDLLGPLYGYQTQEFRIRGKVQTTQEILLSAT
VTPAEMNGLGDKLGQIKPGFIADLLMMKSNPLDDITILDEPESNILFVMKEGRIY"
gene complement(<33850..>36249)
/gene="OPT1"
/locus_tag="YJL212C"
/gene_synonym="DUG4; GSH11; HGT1"
/db_xref="GeneID:853218"
mRNA complement(<33850..>36249)
/gene="OPT1"
/locus_tag="YJL212C"
/gene_synonym="DUG4; GSH11; HGT1"
/product="oligopeptide transporter OPT1"
/transcript_id="NM_001181645.1"
/db_xref="GeneID:853218"
CDS complement(33850..36249)
/gene="OPT1"
/locus_tag="YJL212C"
/gene_synonym="DUG4; GSH11; HGT1"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:19496824]"
/experiment="EXISTENCE:direct assay:GO:0006857
oligopeptide transport [PMID:16149917]"
/experiment="EXISTENCE:mutant phenotype:GO:0005886 plasma
membrane [PMID:10788431]"
/experiment="EXISTENCE:mutant phenotype:GO:0035673
oligopeptide transmembrane transporter activity
[PMID:10652283]"
/note="Proton-coupled oligopeptide transporter of the
plasma membrane; also transports glutathione and
phytochelatin; member of the OPT family"
/codon_start=1
/product="oligopeptide transporter OPT1"
/protein_id="NP_012323.1"
/db_xref="GeneID:853218"
/db_xref="SGD:S000003748"
/translation="MSTIYRESDSLESEPSPTPTTIPIQINMEEEKKDAFVKNIDEDV
NNLTATTDEEDRDPESQKFDRHSIQEEGLVWKGDPTYLPNSPYPEVRSAVSIEDDPTI
RLNHWRTWFLTTVFVVVFAGVNQFFSLRYPSLEINFLVAQVVCYPIGRILALLPDWKC
SKVPFFDLNPGPFTKKEHAVVTIAVALTSSTAYAMYILNAQGSFYNMKLNVGYQFLLV
WTSQMIGYGAAGLTRRWVVNPASSIWPQTLISVSLFDSLHSRKVEKTVANGWTMPRYR
FFLIVLIGSFIWYWVPGFLFTGLSYFNVILWGSKTRHNFIANTIFGTQSGLGALPITF
DYTQVSQAMSGSVFATPFYVSANTYASVLIFFVIVLPCLYFTNTWYAKYMPVISGSTY
DNTQNKYNVTKILNEDYSINLEKYKEYSPVFVPFSYLLSYALNFAAVIAVFVHCILYH
GKDIVAKFKDRKNGGTDIHMRIYSKNYKDCPDWWYLLLQIVMIGLGFVAVCCFDTKFP
AWAFVIAILISLVNFIPQGILEAMTNQHVGLNIITELICGYMLPLRPMANLLFKLYGF
IVMRQGLNLSRDLKLAMYMKVSPRLIFAVQIYATIISGMVNVGVQEWMMHNIDGLCTT
DQPNGFTCANGRTVFNASIIWSLPKYLFSSGRIYNPLMWFFLIGLLFPLAVYAVQWKF
PKFKFAKHIHTPVFFTGPGNIPPSTPYNYSLFFAMSFCLNLIRKRWRAWFNKYNFVMG
AGVEAGVAISVVIIFLCVQYPGGKLSWWGNNVWKRTYDNDYKKFYTLKKGETFGYDKW
W"
gene <36919..>37734
/gene="PEX2"
/locus_tag="YJL210W"
/gene_synonym="CRT1; PAS5"
/db_xref="GeneID:853219"
mRNA <36919..>37734
/gene="PEX2"
/locus_tag="YJL210W"
/gene_synonym="CRT1; PAS5"
/product="ubiquitin-protein ligase peroxin 2"
/transcript_id="NM_001181643.1"
/db_xref="GeneID:853219"
CDS 36919..37734
/gene="PEX2"
/locus_tag="YJL210W"
/gene_synonym="CRT1; PAS5"
/EC_number="2.3.2.36"
/experiment="EXISTENCE:direct assay:GO:0005778 peroxisomal
membrane [PMID:35768507|PMID:12876220]"
/experiment="EXISTENCE:direct assay:GO:0006513 protein
monoubiquitination [PMID:35768507]"
/experiment="EXISTENCE:direct assay:GO:0008320 protein
transmembrane transporter activity [PMID:35768507]"
/experiment="EXISTENCE:direct assay:GO:0016562 protein
import into peroxisome matrix, receptor recycling
[PMID:35768507]"
/experiment="EXISTENCE:direct assay:GO:0044721 protein
import into peroxisome matrix, substrate release
[PMID:35768507]"
/experiment="EXISTENCE:direct assay:GO:0061630 ubiquitin
protein ligase activity
[PMID:35768507|PMID:22471590|PMID:19687296]"
/experiment="EXISTENCE:direct assay:GO:1990429 peroxisomal
importomer complex [PMID:35768507|PMID:22375831]"
/experiment="EXISTENCE:mutant phenotype:GO:0016558 protein
import into peroxisome matrix [PMID:15536088]"
/note="RING-finger peroxin and E3 ubiquitin ligase;
peroxisomal membrane protein with a C-terminal
zinc-binding RING domain, forms translocation subcomplex
with Pex10p and Pex12p which functions in peroxisomal
matrix protein import"
/codon_start=1
/product="ubiquitin-protein ligase peroxin 2"
/protein_id="NP_012325.1"
/db_xref="GeneID:853219"
/db_xref="SGD:S000003746"
/translation="MSRVAQLDSIALDKELYGQFWSEFNAAFNTSEHKEEWELALNTV
VFMCATRFLPHYGSSCTYGSALSGVVFQCRKRTLYVVTVLAGYVWKKITHIIFNGPHC
GNQMMWLKLYKWVNLLYHGCDVTNFLRFLAAEGPNARAFLSPLYRAFNVHSTRLIRDG
SAIASEFYSNSVFAGLEYQNRQLLWNALLELFSNTLLTKRGLLTFVKKPPRSRSTTTY
KTVCPRCGGFPTNPYQIACCRANYCYVCVVKALEWSMCDACGSSGRLTASPVY"
gene <38005..>39969
/gene="CBP1"
/locus_tag="YJL209W"
/db_xref="GeneID:853221"
mRNA <38005..>39969
/gene="CBP1"
/locus_tag="YJL209W"
/product="Cbp1p"
/transcript_id="NM_001181642.1"
/db_xref="GeneID:853221"
CDS 38005..39969
/gene="CBP1"
/locus_tag="YJL209W"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:9343381]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:2104848|PMID:22842922]"
/experiment="EXISTENCE:mutant phenotype:GO:0000958
mitochondrial mRNA catabolic process [PMID:9343381]"
/experiment="EXISTENCE:mutant phenotype:GO:0009060 aerobic
respiration [PMID:9343381]"
/note="Mitochondrial protein, regulator of COB mRNA
stability and translation; interacts with the
5'-untranslated region of the COB mRNA; found in a complex
at the inner membrane along with Pet309p; localizes to
mitochondrial foci upon DNA replication stress"
/codon_start=1
/product="Cbp1p"
/protein_id="NP_012326.1"
/db_xref="GeneID:853221"
/db_xref="SGD:S000003745"
/translation="MFLPRLVRYRTERFIKMVPTRTLRRINHSSRDPIQKQVLALIKA
NANLNDNDKLKIRKYWSDMADYKSLRKQENSLLESSILHEVKIEDFISFINRTKTSSM
TTRGIYRRECLYQCKKNLDLVNQVVSQVSSVRHQKPLTTQLDTMRWCVDDAIGTGDIV
MAADLFLLYYRLFTDDKKLDEQYAKKIISVLAYPNPLHDHVHLVKYLQLNSLFESITG
GGIKLTRFQLETLSNKALGLSNEAPQLCKAILNKLMNINYSLTNDLKLRDDQVLLAYK
SIDENYRRGNVASVYSIWNKIKEHYVSISAHDSRIIYKVFKICTHNRAYRSICSEMFW
QLTPEYYCNNPLILPAIIDFITKQDSLTMAKELMQNINRYTLPENHHIVWLNKRCLSS
LLRMHLKFNDSNGVDRVLKQITTNFRALSQENYQAIIIHLFKTQNLDHIAKAVKLLDT
IPPGQAMLAYGSIINEVVDWKLASKVKFTDNLMALVNDLLTKAHDFDPDHRNSLWNVV
SALYIKKLCHYKKRDGKFVANAKKDIDLAKLLYINAAKRSKTYWTKSNCNPFIASSPC
DVKLKVNNQNRFTILRNIALSALQIGRTDIFLWACAELYQNGMTIEELKLDWNFILKH
QIRNSEFKTNKEIIQDIKKHGVSAVKRYLR"
gene complement(<40194..>41183)
/gene="NUC1"
/locus_tag="YJL208C"
/db_xref="GeneID:853222"
mRNA complement(<40194..>41183)
/gene="NUC1"
/locus_tag="YJL208C"
/product="ribonuclease"
/transcript_id="NM_001181641.1"
/db_xref="GeneID:853222"
CDS complement(40194..41183)
/gene="NUC1"
/locus_tag="YJL208C"
/experiment="EXISTENCE:direct assay:GO:0004520 DNA
endonuclease activity [PMID:3286639]"
/experiment="EXISTENCE:direct assay:GO:0004529 DNA
exonuclease activity [PMID:3286639]"
/experiment="EXISTENCE:direct assay:GO:0004540 RNA
nuclease activity [PMID:3286639]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:17244531]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:17244531|PMID:3286639]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:31266891]"
/experiment="EXISTENCE:direct assay:GO:0006308 DNA
catabolic process [PMID:3286639]"
/experiment="EXISTENCE:direct assay:GO:0006401 RNA
catabolic process [PMID:3286639]"
/experiment="EXISTENCE:direct assay:GO:0051607 defense
response to virus [PMID:31266891]"
/experiment="EXISTENCE:mutant phenotype:GO:0006309
apoptotic DNA fragmentation [PMID:22727375]"
/experiment="EXISTENCE:mutant phenotype:GO:0006310 DNA
recombination [PMID:8087883]"
/experiment="EXISTENCE:mutant phenotype:GO:0006915
apoptotic process [PMID:17244531]"
/note="Major mitochondrial nuclease; has RNAse and DNA
endo- and exonucleolytic activities; prevents genome
instability by degrading extranuclear DNA sequences; roles
in mitochondrial recombination, apoptosis and maintenance
of polyploidy; involved in fragmentation of genomic DNA
during PND (programmed nuclear destruction); role in
preventing L-A mycovirus pathogenesis; encodes ortholog of
mammalian endoG"
/codon_start=1
/product="ribonuclease"
/protein_id="NP_012327.1"
/db_xref="GeneID:853222"
/db_xref="SGD:S000003744"
/translation="MCSRILLSGLVGLGAGTGLTYLLLNKHSPTQIIETPYPPTQKPN
SNIQSHSFNVDPSGFFKYGFPGPIHDLQNREEFISCYNRQTQNPYWVLEHITPESLAA
RNADRKNSFFKEDEVIPEKFRGKLRDYFRSGYDRGHQAPAADAKFSQQAMDDTFYLSN
MCPQVGEGFNRDYWAHLEYFCRGLTKKYKSVRIVTGPLYLPKKDPIDNKFRVNYEVIG
NPPSIAVPTHFFKLIVAEAPTANPAREDIAVAAFVLPNEPISNETKLTDFEVPIDALE
RSTGLELLQKVPPSKKKALCKEVNCQIVVRDFSNAAIKQSKDVKLLPPPKKRN"
gene complement(<41389..>47433)
/gene="LAA1"
/locus_tag="YJL207C"
/db_xref="GeneID:853223"
mRNA complement(<41389..>47433)
/gene="LAA1"
/locus_tag="YJL207C"
/product="AP-1 complex accessory protein LAA1"
/transcript_id="NM_001181640.1"
/db_xref="GeneID:853223"
CDS complement(41389..47433)
/gene="LAA1"
/locus_tag="YJL207C"
/experiment="EXISTENCE:direct assay:GO:0030136
clathrin-coated vesicle [PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0042147
retrograde transport, endosome to Golgi [PMID:16687571]"
/experiment="EXISTENCE:mutant phenotype:GO:0008104
intracellular protein localization [PMID:16687571]"
/experiment="EXISTENCE:mutant phenotype:GO:0035653
clathrin-coated vesicle cargo loading, AP-1-mediated
[PMID:30523155]"
/experiment="EXISTENCE:mutant phenotype:GO:0042147
retrograde transport, endosome to Golgi [PMID:16687571]"
/note="AP-1 accessory protein; colocalizes with clathrin
to the late-Golgi apparatus; involved in TGN-endosome
transport; physically interacts with AP-1; similar to the
mammalian p200; may interact with ribosomes; YJL207C is a
non-essential gene"
/codon_start=1
/product="AP-1 complex accessory protein LAA1"
/protein_id="NP_012328.1"
/db_xref="GeneID:853223"
/db_xref="SGD:S000003743"
/translation="MANRSLKKVIETSSNNGHDLLTWITTNLEKLICLKEVNDNEIQE
VKEIHTQLDEFVRYISVLENTDDLELHSVFISLSQLYTISIWRLKDEYPGVVFDSAAF
LTNVLCEEDVSIDDGDTDPNQKKKKKKSSTKKKKYIYSPAKDIACTILVQLFENFGSS
ISSLIPLLFNAIFKNLKKIMEKSKYYHATFMTTLLQLFNAILRNSNNDDKILDPATYA
KFSKLSKTVFDSISTDEKDFSVTFVSVLIECWTAHFKQTNFIREHSHDIIETIYSRFT
EGEIGVYGFANDETRIFTAKSLAEILFDYYFSKNILTLQEVWSIYVKIFLNCDTRDVE
SGCFESIIHLINLNLLADNTFLSNSKYLDIVLSLSGVFSSYEVNNRSMNTLSRYLRYF
QHMHEVILPHLNDSAKTQMLYYILGCSDTYQSSSKSDSASNFKYSIDAKPETQWLTLL
QLDFTYVLISDLGSTFTTEENTVKEIRDKLVDLATCEIFTIRVHTVEILKVFLNNCPE
YLSETIENSLRALSTDFKSTGKFIFHKNHGHAFIIANLIKGAESDYISYELIMRITVF
STSFIKNNTTSTSSNLYFKGLLCWILLIGLMNYKDEQYLKLQIPQLFLFWKVLLTHTY
TYHDEDELYKNLEIRNHALTCLLTYLSNTTIDKEMAKQVSYLLTKCSNFNHSIDLKSK
NIDNALLHNENRILQVYLKLEKYINSDFNSSLLILIVKNFSDPNLYTESSSSVLGSLK
DIGNRKVSNKDDMESNIVLESSINTLLRQNNGFAFGLSSKITGDRIVNLSMSSAYKYD
ESISGSWPSKDYNWYNIFEVEVSKPISPILSLDSLILLYGSGSYSQIDRYAPQVTTSL
IDSSMELFSSVFPFLNSKIQYSIMETLNLSMFSKMTTPLRSVAVAANVCSALHNALRI
MQENNLELDYSVGQLIIESIKKIQFFNDIFLTKIKADCVGLLTAAIARTLGDEERQKF
LTEQSRIFIKNVADMDEPYLRMFHVLSLATIFKYNSQYANFEEYFDVIFALMRDPHPV
VHSWSLKAMHILLEKHLVIDLKTAALLLSSMEELLVQDKYGIYGRSTLRCNYNRDFNS
HVAIGEISRTLTETVGPNFLELNTKVLDSFRNITLSMLISNNILNSITSIKMFENIAT
FKMKNILNYEIFILASKSIIKSSIVTGIGSSYFDTTFTGSNELISRTSSLKGAFENFD
LLTLLYKLQMEEFFMKEMENLSWRYLALFPNSGSVKNYFTEWILHTFKRDNHWFDKLY
SIFNMSLGRLFQSYNRDVSALLEVNGLKKSSEKEIKGEEEESIANVNQLTDTDAGGLD
SENLQWKSRQIILNLILMLCLESEKYENLLLALSNKIADLIKISFRGSTVRNEGMKLT
GLHILNFVLKNYSTMRDPQVPGSSILEQQEAQITSALMPAFSKGSSPTVMSFAITVAA
EVLASNIMPPDKLGRISQLLIDLLGNFKDPNSGIRIGEAIIVTPKAKRKIELAVLDAW
AEVVQRSITSSNDALFSFTRKYWSILVPLWIISLREYMMIKYNDNDSTVQVKNDSKEN
SLIEPRSTKIELYEPVWLNFVEALGCTLDSDVQVILASLNDEELEYFLFILFSQCLEA
IVKNIDDHSVKMQVLPALHNVLKSNLCIKSIFEDDIITEVVEIMDRLISTGDSKEEFL
LVDIISDLIIGYSKCNATPETFLQDIDKLYELLRLLMTIISERLPFIKYNVLTSEEDD
NEIKISPTDISLLKKTFIAFESNISNFDNMFKVDLYSCLLFIIGKIYECSHREVIIPI
ILPLFKALVKALTESEDEKNIVLLEIFYGSIKDVIYHKLDSKNKVATILILLSNGYSK
LSFQELNQCANILSEALNNPATQPIALQGFKRIISNIFKYPLLQYFMKLVIKRFFQDI
QTNDSLSQASIKTKLIIQFSEEVIKQDHQKASLSIALCLSFFAAYHSAYTEKIDNEVA
SGIVALAKLDKNSFKEAISSTISPQQKAIIGSVMEAYVKSQSLGSVEEAFQLKSFD"
gene complement(<47659..>49935)
/locus_tag="YJL206C"
/db_xref="GeneID:853224"
mRNA complement(<47659..>49935)
/locus_tag="YJL206C"
/product="uncharacterized protein"
/transcript_id="NM_001181638.1"
/db_xref="GeneID:853224"
CDS complement(47659..49935)
/locus_tag="YJL206C"
/note="hypothetical protein; similar to transcriptional
regulators from the Zn[2]-Cys[6] binuclear cluster protein
family; mRNA is weakly cell cycle regulated, peaking in S
phase; induced rapidly upon MMS treatment"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_012329.1"
/db_xref="GeneID:853224"
/db_xref="SGD:S000003741"
/translation="MTPKESGKPISCAMKKLKGKRSKILVLSRDAGTNELKPTKGRAH
RACIACRKRKVRCSGNIPCRLCQTNSYECKYDRPPRNSSVFDREVSDDSSLYAQRASH
EREDSKGPISSIDYKKVVETIFPPETLRQILASSSFNSQNFLDTIKTCLLQGQLNVNQ
VIRQSLPKDTPWHMQTSVPLPPREIALKFIQKTWDCACVLFRFYHRPTIISILDSIYE
AEKHGKQYTPEQVKTQPLIYSVLAVGALFSKEDLSKDSKATREFYTDEGYRYFLEAKN
SLDFSNITDIYSIQAIFMMTIFLQCSANLKACYSFIGIALRAALKEGLHRRSSIVGPT
PIQDETKKRLFWSVYKLDLYMNCILGFPSGIDESDIDQEFPLDVDDENISTIGIKFQD
WRTISSCGMNNKHTKLILIMSRIYKLMYSLRRKPLEEDSRTQIVSLNDQLDNWYAQLP
DILKVDTIRYRQTQPPLTVSANDTSSPYTKPKKLLYLDFLLSKIVLYKPFYHYISIDP
LDIPEFQFQIHMAENCIEVAKKVIQLSYEMITQNLLSGSYWFSIHTIFFSVACLKFYV
YQTEKGLIRNGKVDSDIHNATQLGSEILSLLKGASNASKRTFEVLNQLFKEFNEKTSV
LSEQLLNIVKLQRQESSGALVPQLQTNNNFTKCQGELHHGQQHHQTPATSLRSILNLP
QGEADLKFQNTNNESHTTTAAQEEYLDKLLAEFEEFDYSINRVLPDVIDFSALIGQDS
SANNQIFSSEFSSDPTVN"
gene complement(<50139..>50443)
/gene="NCE101"
/locus_tag="YJL205C"
/gene_synonym="NCE1; YJL205C-A; YJL206C-A"
/db_xref="GeneID:853225"
mRNA complement(join(<50139..50268,50412..>50443))
/gene="NCE101"
/locus_tag="YJL205C"
/gene_synonym="NCE1; YJL205C-A; YJL206C-A"
/product="Nce101p"
/transcript_id="NM_001181639.1"
/db_xref="GeneID:853225"
CDS complement(join(50139..50268,50412..50443))
/gene="NCE101"
/locus_tag="YJL205C"
/gene_synonym="NCE1; YJL205C-A; YJL206C-A"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0009306
protein secretion [PMID:8655575]"
/note="hypothetical protein; involved in secretion of
proteins that lack classical secretory signal sequences;
SWAT-GFP and mCherry fusion proteins localize to the
cytosol"
/codon_start=1
/product="Nce101p"
/protein_id="NP_012330.1"
/db_xref="GeneID:853225"
/db_xref="SGD:S000003742"
/translation="MVQYAPFLLGKFSDPLLAIMVGCLSYYVYERKMGRPQGHHLHEL
IKKRWDDRK"
gene complement(<50629..>53151)
/gene="RCY1"
/locus_tag="YJL204C"
/db_xref="GeneID:853226"
mRNA complement(<50629..>53151)
/gene="RCY1"
/locus_tag="YJL204C"
/product="Rcy1p"
/transcript_id="NM_001181637.1"
/db_xref="GeneID:853226"
CDS complement(50629..53151)
/gene="RCY1"
/locus_tag="YJL204C"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:15537705]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:15537705]"
/experiment="EXISTENCE:direct assay:GO:0005933 cellular
bud [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0030427 site of
polarized growth [PMID:11287615]"
/experiment="EXISTENCE:mutant phenotype:GO:0000149 SNARE
binding [PMID:11287615]"
/experiment="EXISTENCE:mutant phenotype:GO:0006897
endocytosis [PMID:11378903|PMID:10769031]"
/experiment="EXISTENCE:mutant phenotype:GO:0032456
endocytic recycling [PMID:24272750]"
/experiment="EXISTENCE:mutant phenotype:GO:0034498 early
endosome to Golgi transport [PMID:15537705]"
/note="F-box protein involved in recycling endocytosed
proteins; involved in recycling plasma membrane proteins
internalized by endocytosis; localized to sites of
polarized growth; direct interaction with C-terminal
cytoplasmic region of Drs2p plays an important role for
Drs2p function in endocytic recycling pathway; required
for turnover of histone H3 variant Cse4p"
/codon_start=1
/product="Rcy1p"
/protein_id="NP_012331.1"
/db_xref="GeneID:853226"
/db_xref="SGD:S000003740"
/translation="MDDLLKVPEIVTNIASYLSTVDYLSFQQVNKRVYAIINGKNDSK
YWSLKLTRMGLQQVHSNEEEEITLLDENDNQNSLRIFEIYKSFTAQNSKKIFVKFYRC
YNSYARKLYNNNLANFFPTSYSNDPLKQTRILNFIKKYNFSNKNDIETFTRIETNFNI
LREIFINSVLKESELNYQSNNLAAVARFMKILLISNEESNAIEFFKSKADLPPSLTVL
PSNDELFWAEQPREEDSGGSTVIFNSKNLDTFLNQLRDFLNEKIKLADILFKDEFPVI
LQFIESFIQDILLDILNNILLSYSEFLKENGKDSKANYECVPELYFTFIKKFDTELND
SVNAGANFRKVVRDLLNLYLEPFVVNYMNQTTRVFESLINSQLANYDTQVQDKQREQN
AKIYNTLKDQTDASSASNNELPNDLSIITETSKTVPEADSKPSTIHQSVHSTDISNDK
LDFLSSFTKIFKFSNNENQRLKQQLQLAYNLNLISNNLQNIKSLISLDLCYKILQETS
EKTDQIYKFHTIESLLPLIKLRCQEIFKILITQLNKNHVKPAFEKAILLLQKYNPNEI
EQIEIKFNSLSPANTQVEPLVQFTELINIGDIILQMISIFYKNELIPKKIIDKNKDFL
NDVIQLKKNFETSIDDFVAEGLNIGINKLMDEISFVFKTLQLPDDYNPPPPSRNSPIR
DIKPTKCAIRVVELLSNHCFLLTGATDKGTIDVYQQEIGERFFNEIVKHLKKCFISTE
GAIWLICDLNYFYDFIANKLKQKNVVPYFVGLKSIGQLYIISGKDSKELGKLISDLGK
FNGIFTQEEIYEFVQRRSDWVRVRKDVEKVMYGLGIRDCCIM"
gene <53341..>54183
/gene="PRP21"
/locus_tag="YJL203W"
/gene_synonym="SPP91"
/db_xref="GeneID:853227"
mRNA <53341..>54183
/gene="PRP21"
/locus_tag="YJL203W"
/gene_synonym="SPP91"
/product="Prp21p"
/transcript_id="NM_001181636.1"
/db_xref="GeneID:853227"
CDS 53341..54183
/gene="PRP21"
/locus_tag="YJL203W"
/gene_synonym="SPP91"
/experiment="EXISTENCE:direct assay:GO:0000398 mRNA
splicing, via spliceosome [PMID:8969185]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:8065365]"
/experiment="EXISTENCE:direct assay:GO:0071004 U2-type
prespliceosome [PMID:16618970]"
/note="Subunit of the SF3a splicing factor complex;
required for spliceosome assembly"
/codon_start=1
/product="Prp21p"
/protein_id="NP_012332.1"
/db_xref="GeneID:853227"
/db_xref="SGD:S000003739"
/translation="MEPEDTQLKEDIKTTVNYIKQHGVEFENKLLEDERFSFIKKDDP
LHEYYTKLMNEPTDTVSGEDNDRKSEREIARPPDFLFSQYDTGISRRDMEVIKLTARY
YAKDKSIVEQMISKDGEARLNFMNSSHPLHKTFTDFVAQYKRVYSFTGQEIKKSKRTI
LDNCFERTQYWEFEKDKDREHDKLVELCKIQFAAIPWDKFTQVAKFSIPEDTEIFEGS
LDLEQMRLRRVQTGIKLFDSIKPTNEEEKIVSDQGKQKGGDSKGKKRKIRAVGETRLK
KSKK"
gene <54379..>56178
/gene="ECM25"
/locus_tag="YJL201W"
/db_xref="GeneID:853229"
mRNA <54379..>56178
/gene="ECM25"
/locus_tag="YJL201W"
/product="Ecm25p"
/transcript_id="NM_001181634.1"
/db_xref="GeneID:853229"
CDS 54379..56178
/gene="ECM25"
/locus_tag="YJL201W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0042805 actinin
binding [PMID:34010635]"
/experiment="EXISTENCE:mutant phenotype:GO:0051510
regulation of unidimensional cell growth [PMID:34010635]"
/experiment="EXISTENCE:mutant phenotype:GO:0080135
regulation of cellular response to stress [PMID:34010635]"
/note="Endocytic site protein required for stress-induced
cell elongation; may link the polarization signal with the
endocytic machinery to facilitate adaptive morphogenesis
under conditions of stress; interacts with the polarity
regulator Cdc42p, the SH3 domains of actin patch
components and actin filaments; contains a Rho-GAP domain
lacking the catalytic arginine finger, PXXP motifs and an
xACT (F-actin binding) motif; promoter contains a
consensus binding sequence for transcription factor Abf1p"
/codon_start=1
/product="Ecm25p"
/protein_id="NP_012334.1"
/db_xref="GeneID:853229"
/db_xref="SGD:S000003737"
/translation="MIDINVNNIFFRSYSVDPNSGHAIYVFDSTYLPASDEIGDKQVY
DLLINALMDRLVMKLPQAPYSLVIFSSGFSQRKISWVYGIKMFAKLPKETKFYLQKIF
IVHESFFVRSVYQVISNAMNFNFLDSKDSQHDFPSLVHVLDLTSLSELIDITRLRISL
NVYLYDYQIREHINVPEEYYNRLTPLAIRQYRQLVFDKIFKKLQNDALLCELIFQKPG
NYKKVNIFLDIIKRNNYIDLSQWDIYSLASVWLNYFIKNKAKPLIPIELIPLPIVDDL
KFTSETFRKIIKFNQYQDLFMVIIPFFNRIIAHGESTKHDSRTLSKALTPALCKEKLS
MMTNDRLAIGSRYIKNLLDFFPEIAKEISSPPSSVSSSSTIPVLPKPRKSSPTRYSEL
GCLTLPRSRSPSPQRSVTSPTYTPVALQNTPVLKPKSSSRNVSSPSFNAKPPLPIKAV
TRPQLSLTSNSNTDLALASSSTDTLSSPTKTPSADSLPLSNSSTDLTISDNIKEMVKD
EPAKDKNSVETDIFVQQFESLTLVQNAKIKKFDKELQEKKKKNETTSKTADKFSQKGY
SDIKASNKVSRLAALYEERLQGLQVMNEMKQRW"
gene complement(<56444..>58813)
/gene="ACO2"
/locus_tag="YJL200C"
/db_xref="GeneID:853230"
mRNA complement(<56444..>58813)
/gene="ACO2"
/locus_tag="YJL200C"
/product="aconitate hydratase ACO2"
/transcript_id="NM_001181633.1"
/db_xref="GeneID:853230"
CDS complement(56444..58813)
/gene="ACO2"
/locus_tag="YJL200C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:14562095|PMID:14576278|PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:mutant phenotype:GO:0006099
tricarboxylic acid cycle [PMID:30834327]"
/note="Putative mitochondrial aconitase isozyme;
similarity to Aco1p, an aconitase required for the TCA
cycle; expression induced during growth on glucose, by
amino acid starvation via Gcn4p, and repressed on ethanol"
/codon_start=1
/product="aconitate hydratase ACO2"
/protein_id="NP_012335.1"
/db_xref="GeneID:853230"
/db_xref="SGD:S000003736"
/translation="MLSSANRFYIKRHLATHANMFPSVSKNFQTKVPPYAKLLTNLDK
IKQITNNAPLTLAEKILYSHLCDPEESITSSDLSTIRGNKYLKLNPDRVAMQDASAQM
ALLQFMTTGLNQTSVPASIHCDHLIVGKDGETKDLPSSIATNQEVFDFLESCAKRYGI
QFWGPGSGIIHQIVLENFSAPGLMMLGTDSHTPNAGGLGAIAIGVGGADAVDALTGTP
WELKAPKILGVKLTGKLNGWSTPKDVITKLAGLLTVRGGTGYIVEYFGEGVSTLSCTG
MATICNMGAEIGATTSTFPYQEAHKRYLQATNRAEVAEAADVALNKFNFLRADKDAQY
DKVIEIDLSAIEPHVNGPFTPDLSTPISQYAEKSLKENWPQKVSAGLIGSCTNSSYQD
MSRVVDLVKQASKAGLKPRIPFFVTPGSEQIRATLERDGIIDIFQENGAKVLANACGP
CIGQWNREDVSKTSKETNTIFTSFNRNFRARNDGNRNTMNFLTSPEIVTAMSYSGDAQ
FNPLTDSIKLPNGKDFKFQPPKGDELPKRGFEHGRDKFYPEMDPKPDSNVEIKVDPNS
DRLQLLEPFKPWNGKELKTNVLLKVEGKCTTDHISAAGVWLKYKGHLENISYNTLIGA
QNKETGEVNKAYDLDGTEYDIPGLMMKWKSDGRPWTVIAEHNYGEGSAREHAALSPRF
LGGEILLVKSFARIHETNLKKQGVLPLTFANESDYDKISSGDVLETLNLVDMIAKDGN
NGGEIDVKITKPNGESFTIKAKHTMSKDQIDFFKAGSAINYIGNIRRNE"
gene complement(59100..59172)
/locus_tag="YNCJ0001C"
/db_xref="GeneID:853239"
tRNA complement(59100..59172)
/locus_tag="YNCJ0001C"
/product="tRNA-Thr"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:853239"
/db_xref="SGD:S000006743"
repeat_region 59505..59784
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007027"
gene complement(<59856..>60182)
/gene="MBB1"
/locus_tag="YJL199C"
/db_xref="GeneID:853240"
mRNA complement(<59856..>60182)
/gene="MBB1"
/locus_tag="YJL199C"
/product="Mbb1p"
/transcript_id="NM_001348846.1"
/db_xref="GeneID:853240"
CDS complement(59856..60182)
/gene="MBB1"
/locus_tag="YJL199C"
/note="hypothetical protein; conserved among S. cerevisiae
strains, not conserved in closely related Saccharomyces
species; protein detected via large-scale protein-protein
interaction studies and enrichment-based proteogenomics;
YJL199C is not an essential gene"
/codon_start=1
/product="Mbb1p"
/protein_id="NP_001335786.1"
/db_xref="GeneID:853240"
/db_xref="SGD:S000003735"
/translation="MYKVSACGVRIMSGISEIWIGELRDYKYALRLDREEYPAVLVYE
YDSSSTRNYLFTIKYSDQRPNWQLITRDAALTAYDLLNRGGEFTTLSFPGATIHRSVS
ELSRLH"
gene <60844..>63489
/gene="PHO90"
/locus_tag="YJL198W"
/db_xref="GeneID:853241"
mRNA <60844..>63489
/gene="PHO90"
/locus_tag="YJL198W"
/product="SPX domain-containing inorganic phosphate
transporter"
/transcript_id="NM_001181631.1"
/db_xref="GeneID:853241"
CDS 60844..63489
/gene="PHO90"
/locus_tag="YJL198W"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:21143198]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0005315
phosphate transmembrane transporter activity
[PMID:11779791]"
/experiment="EXISTENCE:genetic interaction:GO:0006817
phosphate ion transport [PMID:11779791]"
/experiment="EXISTENCE:genetic interaction:GO:2000185
regulation of phosphate transmembrane transport
[PMID:12821119]"
/experiment="EXISTENCE:mutant phenotype:GO:0005315
phosphate transmembrane transporter activity
[PMID:12581367]"
/experiment="EXISTENCE:mutant phenotype:GO:0006797
polyphosphate metabolic process [PMID:17804816]"
/note="Low-affinity phosphate transporter; acts upstream
of Pho81p in regulation of the PHO pathway; deletion of
pho84, pho87, pho89, pho90, and pho91 causes synthetic
lethality; transcription independent of Pi and Pho4p
activity; overexpression results in vigorous growth; PHO90
has a paralog, PHO87, that arose from the whole genome
duplication"
/codon_start=1
/product="SPX domain-containing inorganic phosphate
transporter"
/protein_id="NP_012337.1"
/db_xref="GeneID:853241"
/db_xref="SGD:S000003734"
/translation="MRFSHFLKYNAVPEWQNHYMDYSELKNLIYTLQTDELQVGDNEE
GFGAGKSSNITDRFKNKFSFKNAKEDTSSGMNKDAGIVEETIELRELPTAQTVAAKPS
PFRRMKEKIFYKRRSSSASSVSSTANENLQLDTYDTFVGDLTAEKQKVDDFYKRTEAK
FYDKFDALVKDLKKIGVIEYDIDDDTLFNEPIASTNDEVPPLDLDDDEDDDEFYDDQS
NIEDNTALLHHSQYNIKSQKKSLLKKSIVNLYIDLCQLKSFIELNRIGFAKITKKSDK
VLHLNTRTELIESEQFFKDTYAFQAETIELLNSKISQLVTFYARITDRPHNISHSKQE
LKSYLHDHIVWERSNTWKDMLGLLSQADELTPKETEYNANKLVGKLDLEYYRWPLPRP
INLKFTSINNVALPKLFFTKKAYKIYFIILVTGLLLGIKTFNDAAQHRCMALVECVAF
LWASEAIPLHITAFLVPLLVVLFKVLKTSDGAIMSAASASSEILAAMWSSTIMILLAG
FTLGEVLAQYNIAKVLASWLLAFAGCKPRNVLLMAMCVVFFLSMWISNVAAPVLTYSL
LSPLLDAMDADSPFAQALVLGVALAANIGGMSSPISSPQNIISMSYLKPYGIGWGQFF
AVALPSGILAMLLVWILLFTTFKMNKTKLEKFKPIKTKFTVKQYYIITVTVATILLWC
VESQIEGAFGSSGQIAIIPIVLFFGTGLLSTQDLNAFPWSIVILAMGGIALGKAVSSS
GLLSTIAKALQKKIENDGVFAILCIFGILMLVVGTFVSHTVSAIIIIPLVQEVGDKLG
NPKAAPILVFGCALLSSCGMGLASSGFPNVTAISKVDRKGDRYLSVMTFLTRGVPASI
LAFLCVITLGYGIMASVVKGNATSA"
gene <63805..>67569
/gene="UBP12"
/locus_tag="YJL197W"
/db_xref="GeneID:853242"
mRNA <63805..>67569
/gene="UBP12"
/locus_tag="YJL197W"
/product="putative ubiquitin-specific protease UBP12"
/transcript_id="NM_001181630.1"
/db_xref="GeneID:853242"
CDS 63805..67569
/gene="UBP12"
/locus_tag="YJL197W"
/EC_number="3.4.19.12"
/experiment="EXISTENCE:direct assay:GO:0004843
cysteine-type deubiquitinase activity [PMID:29309037]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0043596 nuclear
replication fork [PMID:31665643]"
/experiment="EXISTENCE:genetic interaction:GO:0006974 DNA
damage response [PMID:31665643]"
/experiment="EXISTENCE:genetic interaction:GO:0016579
protein deubiquitination [PMID:31665643]"
/experiment="EXISTENCE:mutant phenotype:GO:0010637
negative regulation of mitochondrial fusion
[PMID:23317502]"
/experiment="EXISTENCE:mutant phenotype:GO:0016579 protein
deubiquitination [PMID:23317502|PMID:29309037]"
/note="Ubiquitin-specific protease; cleaves ubiquitin from
ubiquitinated proteins; present in the nucleus and
cytoplasm"
/codon_start=1
/product="putative ubiquitin-specific protease UBP12"
/protein_id="NP_012338.1"
/db_xref="GeneID:853242"
/db_xref="SGD:S000003733"
/translation="MGSSDVSSRECSLVYNEDPDFTDGTTPCDRLGVDLMNVLDDKDE
IKQESVPVSDREIEDTESDASAVSSFASANELIAEPHAASETNLGTNGQDGRNVLEQQ
RDVVARLIEENKETQKEGDKVCIVPKVWYDKFFDPDVTDPEDIGPINTRMICRDFENF
VLEDYNRCPYLSIAEPVFNFLSEIYGMTSGSYPVVTNLVINQTTGELETEYNKWFFRL
HYLTEKQDGRKRRHGQDDSIMYLSMSALNLVRDLVEKSMNLFFEKADHLDVNAVDFKI
WFVSEGSDIATDSNVSTFLNSSYEITPLQFLELPIKKLLIPDMFENRLDKITSNPSDL
VIEIKPIEGNHHWPSNYFAYNKLEPASGTTGLVNLGNTCYMNSALQCLVHIPQLRDYF
LYDGYEDEINEENPLGYHGYVARAFSDLVQKLFQNRMSIMQRNAAFPPSMFKSTIGHF
NSMFSGYMQQDSQEFLAFLLDSLHEDLNRIIKKEYTEKPSLSPGDDVNDWNVVKKLAD
DTWEMHLKRNCSVITDLFVGMYKSTLYCPECQNVSITFDPYNDVTLPLPVDTVWDKTI
KIFPMNSPPLLLEVELSKSSTYMDLKNYVGKMSGLDPNTLFGCEIFSNQIYVNYESTE
SNAQFLTLQELIKPADDVIFYELPVTNDNEVIVPVLNTRIEKGYKNAMLFGVPFFITL
KEDELNNPGAIRMKLQNRFVHLSGGYIPFPEPVGNRTDFADAFPLLVEKYPDVEFEQY
KDILQYTSIKVTDKDKSFFSIKILSVEKEQQFASNNRTGPNFWTPISQLNLDKATDID
DKLEDVVKDIYNYSSLVDCAEGVLMQVDDEGDTEGSEAKNFSKPFQSGDDEENKETVT
NNENVNNTNDRDEDMELTDDVEEDASTEPELTDKPEALDKIKDSLTSTPFAILSMNDI
IVCEWSELGSNEAFSDDKIYNWENPATLPNKELENAKLERSNAKERTITLDDCLQLFS
KPEILGLTDSWYCPTCKEHRQATKQIQLWNTPDILLIHLKRFESQRSFSDKIDATVNF
PITDLDLSRYVVYKDDPRGLIYDLYAVDNHYGGLGGGHYTAYVKNFADNKWYYFDDSR
VTETAPENSIAGSAYLLFYIRRHKDGNGLGSSKLQEIIQKSRHGYDERIKKIYDEQMK
LYEFNKTDEEEDVSDDMIECNEDVQAPEYSNRSLEVGHIETQDCNDEDDNDDGERTNS
GRRKLRLLKKVYKNNSGLGSSSTSEISEGCPENEVADLNLKNGVTLESPE"
rep_origin 67663..67736
/note="ARS1005; Autonomously Replicating Sequence"
/db_xref="SGD:S000028442"
gene complement(<67850..>68782)
/gene="ELO1"
/locus_tag="YJL196C"
/db_xref="GeneID:853243"
mRNA complement(<67850..>68782)
/gene="ELO1"
/locus_tag="YJL196C"
/product="fatty acid elongase ELO1"
/transcript_id="NM_001181629.1"
/db_xref="GeneID:853243"
CDS complement(67850..68782)
/gene="ELO1"
/locus_tag="YJL196C"
/EC_number="2.3.1.199"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0009922 fatty
acid elongase activity [PMID:12684876]"
/experiment="EXISTENCE:mutant phenotype:GO:0019368 fatty
acid elongation, unsaturated fatty acid [PMID:10850979]"
/note="Elongase I, medium-chain acyl elongase; catalyzes
carboxy-terminal elongation of unsaturated C12-C16 fatty
acyl-CoAs to C16-C18 fatty acids; ELO1 has a paralog,
ELO2, that arose from the whole genome duplication"
/codon_start=1
/product="fatty acid elongase ELO1"
/protein_id="NP_012339.1"
/db_xref="GeneID:853243"
/db_xref="SGD:S000003732"
/translation="MVSDWKNFCLEKASRFRPTIDRPFFNIYLWDYFNRAVGWATAGR
FQPKDFEFTVGKQPLSEPRPVLLFIAMYYVVIFGGRSLVKSCKPLKLRFISQVHNLML
TSVSFLWLILMVEQMLPIVYRHGLYFAVCNVESWTQPMETLYYLNYMTKFVEFADTVL
MVLKHRKLTFLHTYHHGATALLCYNQLVGYTAVTWVPVTLNLAVHVLMYWYYFLSASG
IRVWWKAWVTRLQIVQFMLDLIVVYYVLYQKIVAAYFKNACTPQCEDCLGSMTAIAAG
AAILTSYLFLFISFYIEVYKRGSASGKKKINKNN"
gene <69338..>70879
/gene="CDC6"
/locus_tag="YJL194W"
/db_xref="GeneID:853244"
mRNA <69338..>70879
/gene="CDC6"
/locus_tag="YJL194W"
/product="AAA family ATPase CDC6"
/transcript_id="NM_001181627.1"
/db_xref="GeneID:853244"
CDS 69338..70879
/gene="CDC6"
/locus_tag="YJL194W"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:9159120|PMID:9288745]"
/experiment="EXISTENCE:direct assay:GO:0003688 DNA
replication origin binding [PMID:9288745|PMID:16824194]"
/experiment="EXISTENCE:direct assay:GO:0003924 GTPase
activity [PMID:8083240]"
/experiment="EXISTENCE:direct assay:GO:0004861
cyclin-dependent protein serine/threonine kinase inhibitor
activity [PMID:8930895]"
/experiment="EXISTENCE:direct assay:GO:0005524 ATP binding
[PMID:8083240]"
/experiment="EXISTENCE:direct assay:GO:0005525 GTP binding
[PMID:8083240]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005656 nuclear
pre-replicative complex [PMID:16824194]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0006267
pre-replicative complex assembly involved in nuclear cell
cycle DNA replication [PMID:16824194]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:8083240]"
/experiment="EXISTENCE:direct assay:GO:0031261 DNA
replication preinitiation complex [PMID:9554851]"
/experiment="EXISTENCE:direct assay:GO:0045736 negative
regulation of cyclin-dependent protein serine/threonine
kinase activity [PMID:8930895]"
/experiment="EXISTENCE:mutant phenotype:GO:0000082 G1/S
transition of mitotic cell cycle [PMID:10075735]"
/experiment="EXISTENCE:mutant phenotype:GO:0003682
chromatin binding [PMID:10075735]"
/experiment="EXISTENCE:mutant phenotype:GO:0004861
cyclin-dependent protein serine/threonine kinase inhibitor
activity [PMID:11460169]"
/experiment="EXISTENCE:mutant phenotype:GO:0005524 ATP
binding [PMID:10075735]"
/experiment="EXISTENCE:mutant phenotype:GO:0006267
pre-replicative complex assembly involved in nuclear cell
cycle DNA replication
[PMID:9335335|PMID:8538771|PMID:23376927|PMID:10075735]"
/experiment="EXISTENCE:mutant phenotype:GO:0030174
regulation of DNA-templated DNA replication initiation
[PMID:9990508]"
/experiment="EXISTENCE:mutant phenotype:GO:0045736
negative regulation of cyclin-dependent protein
serine/threonine kinase activity [PMID:11460169]"
/experiment="EXISTENCE:mutant phenotype:GO:0140530 MCM
complex loading [PMID:29795547]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:10075735|PMID:9312054]"
/note="Essential ATP-binding protein required for DNA
replication; component of the pre-replicative complex
(pre-RC) which requires ORC to associate with chromatin
and is in turn required for Mcm2-7p DNA association; dual
role as a mitotic cyclin-dependent kinase inhibitor, that
binds to and inhibits Cdc28p/Clb2p along with Sic1p,
contributing to mitotic exit; homologous to S. pombe
Cdc18p; relocalizes from nucleus to cytoplasm upon DNA
replication stress; degraded in response to plasma
membrane stress"
/codon_start=1
/product="AAA family ATPase CDC6"
/protein_id="NP_012341.1"
/db_xref="GeneID:853244"
/db_xref="SGD:S000003730"
/translation="MSAIPITPTKRIRRNLFDDAPATPPRPLKRKKLQFTDVTPESSP
EKLQFGSQSIFLRTKALLQKSSELVNLNSSDGALPARTAEYEQVMNFLAKAISEHRSD
SLYITGPPGTGKTAQLDMIIRQKFQSLPLSLSTPRSKDVLRHTNPNLQNLSWFELPDG
RLESVAVTSINCISLGEPSSIFQKIFDSFQDLNGPTLQIKNMQHLQKFLEPYHKKTTF
VVVLDEMDRLLHANTSETQSVRTILELFLLAKLPTVSFVLIGMANSLDMKDRFLSRLN
LDRGLLPQTIVFQPYTAEQMYEIVIQKMSSLPTIIFQPMAIKFAAKKCAGNTGDLRKL
FDVLRGSIEIYELEKRFLLSPTRGSLNSAQVPLTPTTSPVKKSYPEPQGKIGLNYIAK
VFSKFVNNNSTRTRIAKLNIQQKLILCTIIQSLKLNSDATIDESFDHYIKAITKTDTL
APLQRNEFLEICTILETCGLVSIKKTKCKGKTKRFVDKIDVDLDMREFYDEMTKISIL
KPFLH"
gene <71366..>72574
/locus_tag="YJL193W"
/db_xref="GeneID:853246"
mRNA <71366..>72574
/locus_tag="YJL193W"
/product="uncharacterized protein"
/transcript_id="NM_001181626.1"
/db_xref="GeneID:853246"
CDS 71366..72574
/locus_tag="YJL193W"
/note="hypothetical protein; predicted to encode a triose
phosphate transporter subfamily member based on
phylogenetic analysis; similar to YOR307C/SLY41; deletion
mutant has a respiratory growth defect"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_012342.1"
/db_xref="GeneID:853246"
/db_xref="SGD:S000003729"
/translation="MFQQLSASIRHNAHIIFLCISWYFISSLASQVTKQVLTVCPLPL
FLGEFQFIYTAVLAWFTCYIAYSFPGFYRIFPNGTFPEYYIDDRETSRAARKESKLSS
LIIPPSKPILQTVLPLGLFQFVGKYFGHTATSLVPVSTVASIKTLSPMFILLLQKILK
ISTLKITLTLIFSLCTLVLGVWIIVQEDNRSPASSNELREFSKYGVICAMISMFIFVL
QNIYGKTVFTYRSQTDESQSNSGFSRQESPLPLYEKLDEKLVAKKKPKSYDKLTLMIY
ISLVGFCLSFGWFITLEFPVLFRYFFQINSSSTVIKAFPVSLFLLNGTFHFIQAMITF
HLLGEVSTLTYSIANLMKRFAIIAVSWVFIGRRITWLQVFGLVLNTLGLFLYERCTSQ
SKIKAKIRPE"
gene complement(<72710..>73414)
/gene="SOP4"
/locus_tag="YJL192C"
/gene_synonym="EMC7"
/db_xref="GeneID:853247"
mRNA complement(<72710..>73414)
/gene="SOP4"
/locus_tag="YJL192C"
/gene_synonym="EMC7"
/product="Sop4p"
/transcript_id="NM_001181625.1"
/db_xref="GeneID:853247"
CDS complement(72710..73414)
/gene="SOP4"
/locus_tag="YJL192C"
/gene_synonym="EMC7"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:12230471|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:12230471]"
/experiment="EXISTENCE:mutant phenotype:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:12230471]"
/note="ER-membrane protein; subunit of evolutionarily
conserved EMC (Endoplasmic Reticulum Membrane Complex)
implicated in ERAD (ER-associated degradation) and proper
assembly of multi-pass transmembrane (TM) proteins; EMC
acts in yeast as an ER-mitochondria tether that interacts
with outer membrane protein Tom5 of TOM (Translocase of
the Mitochondrial Outer Membrane) complex; suppressor of
pma1-7, deletion of SOP4 slows down export of wild-type
Pma1p and Pma1-7 from the ER"
/codon_start=1
/product="Sop4p"
/protein_id="NP_012343.1"
/db_xref="GeneID:853247"
/db_xref="SGD:S000003728"
/translation="MFSQIVLLLSAFIYVASATARRGTIKGRLDLAASNITGFVSTRT
SFKLYQIGNFSTEYPYTSTTMFQDDEGNFEFANLPLNDGVNETTYYVMYPASMDFNLK
PNRILIEFKNLENGTLQLNAFKNFFGREYFPSKDITYPEKLQSMKVHPYITVELLHKA
PIRSYLQARNVSIFSTGIVGNILNSRWKLAGVITLIALVVFPIIVEKLDPETARAIRE
EAKRKQREKYAAVASK"
gene <73787..>74611
/gene="RPS14B"
/locus_tag="YJL191W"
/gene_synonym="CRY2"
/db_xref="GeneID:853248"
mRNA join(<73787..73796,74205..>74611)
/gene="RPS14B"
/locus_tag="YJL191W"
/gene_synonym="CRY2"
/product="40S ribosomal protein uS11 RPS14B"
/transcript_id="NM_001181624.1"
/db_xref="GeneID:853248"
CDS join(73787..73796,74205..74611)
/gene="RPS14B"
/locus_tag="YJL191W"
/gene_synonym="CRY2"
/experiment="EXISTENCE:direct assay:GO:0032040
small-subunit processome [PMID:15590835]"
/experiment="EXISTENCE:direct assay:GO:0070181 small
ribosomal subunit rRNA binding [PMID:9858605]"
/experiment="EXISTENCE:genetic interaction:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16246728]"
/experiment="EXISTENCE:mutant phenotype:GO:0000028
ribosomal small subunit assembly [PMID:2277060]"
/experiment="EXISTENCE:mutant phenotype:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15125836]"
/note="Protein component of the small (40S) ribosomal
subunit; required for ribosome assembly and 20S pre-rRNA
processing; mutations confer cryptopleurine resistance;
homologous to mammalian ribosomal protein S14 and
bacterial S11; RPS14B has a paralog, RPS14A, that arose
from the whole genome duplication"
/codon_start=1
/product="40S ribosomal protein uS11 RPS14B"
/protein_id="NP_012344.1"
/db_xref="GeneID:853248"
/db_xref="SGD:S000003727"
/translation="MANDLVQARDNSQVFGVARIYASFNDTFVHVTDLSGKETIARVT
GGMKVKADRDESSPYAAMLAAQDVAAKCKEVGITAVHVKIRATGGTRTKTPGPGGQAA
LRALARSGLRIGRIEDVTPVPSDSTRKKGGRRGRRL"
gene complement(<74910..>75302)
/gene="RPS22A"
/locus_tag="YJL190C"
/gene_synonym="RPS24"
/db_xref="GeneID:853249"
mRNA complement(<74910..>75302)
/gene="RPS22A"
/locus_tag="YJL190C"
/gene_synonym="RPS24"
/product="40S ribosomal protein uS8 RPS22A"
/transcript_id="NM_001181623.1"
/db_xref="GeneID:853249"
CDS complement(74910..75302)
/gene="RPS22A"
/locus_tag="YJL190C"
/gene_synonym="RPS24"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:6814480]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:6814480]"
/experiment="EXISTENCE:direct assay:GO:0022627 cytosolic
small ribosomal subunit [PMID:6814480]"
/note="Protein component of the small (40S) ribosomal
subunit; homologous to mammalian ribosomal protein S15A
and bacterial S8; RPS22A has a paralog, RPS22B, that arose
from the whole genome duplication"
/codon_start=1
/product="40S ribosomal protein uS8 RPS22A"
/protein_id="NP_012345.1"
/db_xref="GeneID:853249"
/db_xref="SGD:S000003726"
/translation="MTRSSVLADALNAINNAEKTGKRQVLIRPSSKVIIKFLQVMQKH
GYIGEFEYIDDHRSGKIVVQLNGRLNKCGVISPRFNVKIGDIEKWTANLLPARQFGYV
ILTTSAGIMDHEEARRKHVSGKILGFVY"
gene <75933..>76474
/gene="RPL39"
/locus_tag="YJL189W"
/gene_synonym="RPL46; SPB2"
/db_xref="GeneID:853250"
mRNA join(<75933..75938,76325..>76474)
/gene="RPL39"
/locus_tag="YJL189W"
/gene_synonym="RPL46; SPB2"
/product="60S ribosomal protein eL39 RPL39"
/transcript_id="NM_001181622.1"
/db_xref="GeneID:853250"
CDS join(75933..75938,76325..76474)
/gene="RPL39"
/locus_tag="YJL189W"
/gene_synonym="RPL46; SPB2"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:11983894]"
/experiment="EXISTENCE:curator inference:GO:0003735
structural constituent of ribosome [PMID:11983894]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:11983894]"
/note="Ribosomal 60S subunit protein L39; required for
ribosome biogenesis; loss of both Rpl31p and Rpl39p
confers lethality; also exhibits genetic interactions with
SIS1 and PAB1; homologous to mammalian ribosomal protein
L39, no bacterial homolog"
/codon_start=1
/product="60S ribosomal protein eL39 RPL39"
/protein_id="NP_012346.1"
/db_xref="GeneID:853250"
/db_xref="SGD:S000003725"
/translation="MAAQKSFRIKQKMAKAKKQNRPLPQWIRLRTNNTIRYNAKRRNW
RRTKMNI"
gene complement(<76803..>79262)
/gene="SWE1"
/locus_tag="YJL187C"
/gene_synonym="WEE1"
/db_xref="GeneID:853252"
mRNA complement(<76803..>79262)
/gene="SWE1"
/locus_tag="YJL187C"
/gene_synonym="WEE1"
/product="tyrosine protein kinase SWE1"
/transcript_id="NM_001181620.1"
/db_xref="GeneID:853252"
CDS complement(76803..79262)
/gene="SWE1"
/locus_tag="YJL187C"
/gene_synonym="WEE1"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0000086 G2/M
transition of mitotic cell cycle [PMID:8253069]"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0004713 protein
tyrosine kinase activity [PMID:8253069]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:15282802|PMID:10805747]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:15282802|PMID:10805747]"
/experiment="EXISTENCE:direct assay:GO:0044879 mitotic
morphogenesis checkpoint signaling [PMID:8930890]"
/experiment="EXISTENCE:genetic interaction:GO:0000320
re-entry into mitotic cell cycle [PMID:15107621]"
/experiment="EXISTENCE:genetic interaction:GO:0010697
negative regulation of mitotic spindle pole body
separation [PMID:8887667]"
/experiment="EXISTENCE:genetic interaction:GO:0090154
positive regulation of sphingolipid biosynthetic process
[PMID:26634277]"
/experiment="EXISTENCE:mutant phenotype:GO:0000086 G2/M
transition of mitotic cell cycle [PMID:8253069]"
/experiment="EXISTENCE:mutant phenotype:GO:0008361
regulation of cell size [PMID:12089449]"
/experiment="EXISTENCE:mutant phenotype:GO:0010697
negative regulation of mitotic spindle pole body
separation [PMID:8887667]"
/experiment="EXISTENCE:mutant phenotype:GO:0040020
regulation of meiotic nuclear division [PMID:10619027]"
/experiment="EXISTENCE:mutant phenotype:GO:0044879 mitotic
morphogenesis checkpoint signaling [PMID:9744879]"
/experiment="EXISTENCE:mutant phenotype:GO:0090154
positive regulation of sphingolipid biosynthetic process
[PMID:26634277]"
/note="Protein kinase that regulates the G2/M transition;
negative regulator of the Cdc28p kinase; morphogenesis
checkpoint kinase; positive regulator of sphingolipid
biosynthesis via Orm2p; phosphorylates a tyrosine residue
in the N-terminus of Hsp90 in a cell-cycle associated
manner, thus modulating the ability of Hsp90 to chaperone
a selected clientele; localizes to the nucleus and to the
daughter side of the mother-bud neck; homolog of S. pombe
Wee1p; potential Cdc28p substrate"
/codon_start=1
/product="tyrosine protein kinase SWE1"
/protein_id="NP_012348.1"
/db_xref="GeneID:853252"
/db_xref="SGD:S000003723"
/translation="MSSLDEDEEDFEMLDTENLQFMGKKMFGKQAGEDESDDFAIGGS
TPTNKLKFYPYSNNKLTRSTGTLNLSLSNTALSEANSKFLGKIEEEEEEEEEGKDEES
VDSRIKRWSPFHENESVTTPITKRSAEKTNSPISLKQWNQRWFPKNDARTENTSSSSS
YSVAKPNQSAFTSSGLVSKMSMDTSLYPAKLRIPETPVKKSPLVEGRDHKHVHLSSSK
NASSSLSVSPLNFVEDNNLQEDLLFSDSPSSKALPSIHVPTIDSSPLSEAKYHAHDRH
NNQTNILSPTNSLVTNSSPQTLHSNKFKKIKRARNSVILKNRELTNSLQQFKDDLYGT
DENFPPPIIISSHHSTRKNPQPYQFRGRYDNDTDEEISTPTRRKSIIGATSQTHRESR
PLSLSSAIVTNTTSAETHSISSTDSSPLNSKRRLISSNKLSANPDSHLFEKFTNVHSI
GKGQFSTVYQVTFAQTNKKYAIKAIKPNKYNSLKRILLEIKILNEVTNQITMDQEGKE
YIIDYISSWKFQNSYYIMTELCENGNLDGFLQEQVIAKKKRLEDWRIWKIIVELSLAL
RFIHDSCHIVHLDLKPANVMITFEGNLKLGDFGMATHLPLEDKSFENEGDREYIAPEI
ISDCTYDYKADIFSLGLMIVEIAANVVLPDNGNAWHKLRSGDLSDAGRLSSTDIHSES
LFSDITKVDTNDLFDFERDNISGNSNNAGTSTVHNNSNINNPNMNNGNDNNNVNTAAT
KNRLILHKSSKIPAWVPKFLIDGESLERIVRWMIEPNYERRPTANQILQTEECLYVEM
TRNAGAIIQEDDFGPKPKFFI"
gene <80155..>81915
/gene="MNN5"
/locus_tag="YJL186W"
/db_xref="GeneID:853253"
mRNA <80155..>81915
/gene="MNN5"
/locus_tag="YJL186W"
/product="alpha-1,2-mannosyltransferase MNN5"
/transcript_id="NM_001181619.2"
/db_xref="GeneID:853253"
CDS 80155..81915
/gene="MNN5"
/locus_tag="YJL186W"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:9756928]"
/experiment="EXISTENCE:mutant phenotype:GO:0000026
alpha-1,2-mannosyltransferase activity [PMID:9756928]"
/experiment="EXISTENCE:mutant phenotype:GO:0009101
glycoprotein biosynthetic process [PMID:9756928]"
/note="Alpha-1,2-mannosyltransferase; responsible for
addition of the second alpha-1,2-linked mannose of the
branches on the mannan backbone of oligosaccharides,
localizes to an early Golgi compartment"
/codon_start=1
/product="alpha-1,2-mannosyltransferase MNN5"
/protein_id="NP_012349.2"
/db_xref="GeneID:853253"
/db_xref="SGD:S000003722"
/translation="MLIRLKKRKILQVIVSAVVLILFFCSVHNDVSSSWLYGKKLRLP
VLTRSNLKNNFYTTLVQAIVENKPADSSPDLSKLHGAEGCSFANNVAAHDSGHDSDLS
YESLSKCYNLNKTVQESLREVHSKFTDTLSGKLNFSIPQREALFSGSEGIVTIGGGKY
SVLAYTMIKKLRDTGTTLPIEVIIPPQDEGEDDFCKNWLPKFNGKCIYFSDIVPSKPL
SDLKLTHFQLKVFGLIISSFKRIIFLDADNYAVKNLDLAFNTTSFNDTGLILWPDFWR
RVTPPAFYNIIGSSINIGKRVRFVSDDISPVSRYDPFVSNSNDYTPKERQEHFLKHVP
LHDLDGTMPDLSSESGQMVIDKIRHFNTLLLALYYNVYGPTWYYKMISQGTAGEGDKD
TFVAAAHALNMPYYQVRTNFEFDGFFYQKDDYKGLALLQHDFEQDYKQYQKAQQKVKA
NIEEFSKLDPDYTLDNGFLKTLMVNDDGSDLDIMFIHASFYKADPWTLYHENRFIGPN
GEQVRGFRKPHRYGMDFELFLFNDMRGSFCTTPKSQVIKFKYFTDKVNTPEWDAMCEY
LTNHVNYLESTHKEAMGEKN"
gene complement(<82095..>82976)
/gene="ATG36"
/locus_tag="YJL185C"
/db_xref="GeneID:853254"
mRNA complement(<82095..>82976)
/gene="ATG36"
/locus_tag="YJL185C"
/product="Atg36p"
/transcript_id="NM_001181618.1"
/db_xref="GeneID:853254"
CDS complement(82095..82976)
/gene="ATG36"
/locus_tag="YJL185C"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:22643220]"
/experiment="EXISTENCE:mutant phenotype:GO:0000423
mitophagy [PMID:29673596]"
/experiment="EXISTENCE:mutant phenotype:GO:0000425
pexophagy [PMID:22643220]"
/experiment="EXISTENCE:physical interaction:GO:0000425
pexophagy [PMID:22643220]"
/note="Pex3p interacting protein, required for pexophagy;
interacts with Atg8p and Atg11p; mRNA is weakly cell cycle
regulated, peaking in G2 phase; YJL185C is a non-essential
gene"
/codon_start=1
/product="Atg36p"
/protein_id="NP_012350.1"
/db_xref="GeneID:853254"
/db_xref="SGD:S000003721"
/translation="MASVNNYQVDCGSRSARIQPRINNGIHDEESLFEVLELSEEEFE
LDFHRLKSFNDVRVINNPDLSPECTNTAISRDETLESASSAFEVPSDEIAILSISSDS
NKNSPPSEQPAPALRNIRSSSNSDRIDEWCLGSHLFNELHQNVPQSSDGVNHGFPVYS
FKERELYTSAKLKKLTNAQRIAVQKLSRDLYPILRTCYREKTRRQLLTYHHERIFDDI
PSFFPQRDFIFNYYSMPLEFDRLSDVDIDSSSRSRFTDESTGETLNRSPSAASSSLEN
TSWFGWTLLSRFLDREW"
gene <83448..>83819
/gene="GON7"
/locus_tag="YJL184W"
/gene_synonym="LDB6; PCC2"
/db_xref="GeneID:853255"
mRNA <83448..>83819
/gene="GON7"
/locus_tag="YJL184W"
/gene_synonym="LDB6; PCC2"
/product="chromatin DNA-binding EKC/KEOPS complex subunit
GON7"
/transcript_id="NM_001181617.1"
/db_xref="GeneID:853255"
CDS 83448..83819
/gene="GON7"
/locus_tag="YJL184W"
/gene_synonym="LDB6; PCC2"
/experiment="EXISTENCE:direct assay:GO:0000408 EKC/KEOPS
complex [PMID:16564010|PMID:16874308]"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:16874308]"
/experiment="EXISTENCE:direct assay:GO:0031490 chromatin
DNA binding [PMID:16874308]"
/experiment="EXISTENCE:genetic interaction:GO:0000722
telomere maintenance via recombination [PMID:23390378]"
/experiment="EXISTENCE:mutant phenotype:GO:0000032 cell
wall mannoprotein biosynthetic process [PMID:14734024]"
/experiment="EXISTENCE:mutant phenotype:GO:0000723
telomere maintenance [PMID:16564010]"
/experiment="EXISTENCE:physical interaction:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:16874308]"
/note="Component of the EKC/KEOPS protein complex;
EKC/KEOPS complex is required for t6A tRNA modification
and telomeric TG1-3 recombination; may have role in
transcription; implicated in osmotic stress response;
other complex members are Kae1p, Cgi121p, Pcc1p, and
Bud32p"
/codon_start=1
/product="chromatin DNA-binding EKC/KEOPS complex subunit
GON7"
/protein_id="NP_012351.1"
/db_xref="GeneID:853255"
/db_xref="SGD:S000003720"
/translation="MKLPVAQYSAPDGVEKSFAPIRDDPRYMTTEGRTTGPSDHVLNA
GQIDRDKPSEPERTKDGSQLTYLGQLRTQLTGLQDDINEFLTGRMELAKNKKKAGADE
KRIQEEINQLLDGGDGDEDAV"
gene <84068..>85336
/gene="MNN11"
/locus_tag="YJL183W"
/db_xref="GeneID:853256"
mRNA <84068..>85336
/gene="MNN11"
/locus_tag="YJL183W"
/product="alpha-1,6-mannosyltransferase"
/transcript_id="NM_001181616.2"
/db_xref="GeneID:853256"
CDS 84068..85336
/gene="MNN11"
/locus_tag="YJL183W"
/experiment="EXISTENCE:direct assay:GO:0000009
alpha-1,6-mannosyltransferase activity [PMID:10635561]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0009101
glycoprotein biosynthetic process [PMID:10635561]"
/experiment="EXISTENCE:mutant phenotype:GO:0006487 protein
N-linked glycosylation [PMID:23210626]"
/experiment="EXISTENCE:mutant phenotype:GO:0009101
glycoprotein biosynthetic process [PMID:10635561]"
/experiment="EXISTENCE:physical interaction:GO:0000136
mannan polymerase complex [PMID:9430634]"
/note="Subunit of a Golgi mannosyltransferase complex;
this complex also contains Anp1p, Mnn9p, Mnn10p, and
Hoc1p, and mediates elongation of the polysaccharide
mannan backbone; has homology to Mnn10p"
/codon_start=1
/product="alpha-1,6-mannosyltransferase"
/protein_id="NP_012352.2"
/db_xref="GeneID:853256"
/db_xref="SGD:S000003719"
/translation="MAIKPRTKGKTYSSRSVGSQWFNRLGFKQNKYGTCKFLSIITAF
VFILYFFSNRFYPISRSAGASYSPSHGLYINEIPASSRLIYPHVEHVPVLKQMTVRGL
YITRLEVDGSKRLILKPEENALTDEEKKKTTDQILLVKHSFLDHGKLVYRKSNDAPEV
VVVTLIDFENYELETIIQIVQNRVDYAQKHQYGVYIRWIQEFLPVLENQNLAESYEFI
KPLVIRAAMHAFPTAKYIHFVDQDALLMNLDLSLQKYLLDPKIMDLALLKNVPVVANS
NIKTYNHFEYSSAKIIIPHDADGNIDASSFVIANDFYGKALIDYLNDPLLRNFPWDNT
GDKLSAAIGHILQWHPTLLGKTAIVIPKVLASQYDASLDQEGESGNGASNGDVYHYNE
GDLAASFKGCRSRGTCASEIGHMYQKIKKS"
gene <85660..>87495
/gene="RBH1"
/locus_tag="YJL181W"
/db_xref="GeneID:853257"
mRNA <85660..>87495
/gene="RBH1"
/locus_tag="YJL181W"
/product="Rbh1p"
/transcript_id="NM_001181614.1"
/db_xref="GeneID:853257"
CDS 85660..87495
/gene="RBH1"
/locus_tag="YJL181W"
/note="hypothetical protein; expression is cell-cycle
regulated as shown by microarray analysis; potential
regulatory target of Mbp1p, which binds to the YJL181W
promoter region; contains a PH-like domain; RBH1 has a
paralog, RBH2, that arose from the whole genome
duplication"
/codon_start=1
/product="Rbh1p"
/protein_id="NP_012354.1"
/db_xref="GeneID:853257"
/db_xref="SGD:S000003717"
/translation="MEIFKEEEEEAFSAIEGIIYACEVYDPVPRHLHKSKTKIINAAK
LIIETHLSYYTILNNISDIQAYLSTWLRDLGTTGPYQTILSESISLMFDRTVSIFRKC
TIEGGFPHLIARLYLRLKSYQKLLNDAGLKNFFSSYDYAFGVAYNLVNCSEYRYDEVH
YISNGTYSLVASMKIDPAEVIKREHFRLTIPKFNISNILIEIFHLLDGLAFFKVNPDS
LSISTASAETIFRSISEGNHQVLELGRSLMFPLLRTGDFEICRIDDAGAVITFTEAKD
VKLEIISLDEVSWVMQWKSCLQNYERRAANDSSFIKTHLQFKKANNFNEDNNGLGLIV
DRNIPTDDFTLASTNRQSPPPSNTGCSLHRSKPLHIPLSSVIREDFYDSSLNERISKD
GDSSCESFSGAESILSDYDFHDNEFFNNQSPHYFSEHIDNNSREVVITDENTIISLEN
TQVSRWSNYSWQKISPHQLQVSIIQLRMGNFIVAYDSDYNLHQFKIRLCDDIKCIQST
EQDIQIRVPLGAIMCSVTGILNIRTKDADKLLRVLSFYTTDHTEAVSHSNNQDATASP
LSSVSSAMDLKHSLQKCSSTIMPQELTQDVIGSKSDLISNIRQKI"
gene complement(<87583..>88560)
/gene="ATP12"
/locus_tag="YJL180C"
/db_xref="GeneID:853259"
mRNA complement(<87583..>88560)
/gene="ATP12"
/locus_tag="YJL180C"
/product="ATP synthase complex assembly protein ATP12"
/transcript_id="NM_001181613.1"
/db_xref="GeneID:853259"
CDS complement(87583..88560)
/gene="ATP12"
/locus_tag="YJL180C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:1826907|PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:mutant phenotype:GO:0033615
mitochondrial proton-transporting ATP synthase complex
assembly [PMID:2142305]"
/experiment="EXISTENCE:physical interaction:GO:0019904
protein domain specific binding [PMID:10747017]"
/experiment="EXISTENCE:physical interaction:GO:0033615
mitochondrial proton-transporting ATP synthase complex
assembly [PMID:10747017]"
/note="Assembly factor for F1 sector of mitochondrial F1F0
ATP synthase; conserved protein; required for assembly of
alpha and beta subunits into F1 sector of mitochondrial
F1F0 ATP synthase; human homolog ATPAF2 can complement
yeast atp12 mutant; mutation of human homolog reduces
active ATP synthase levels and is associated with the
disorder ATPAF2 deficiency"
/codon_start=1
/product="ATP synthase complex assembly protein ATP12"
/protein_id="NP_012355.1"
/db_xref="GeneID:853259"
/db_xref="SGD:S000003716"
/translation="MLPSLRKGCFIVNSIRLKLPRFYSLNAQPLGTDNTIENNTPTET
NRLSKTSQKFWEKVSLNRDVEKGKIALQLDGRTIKTPLGNGIIVDNAKSLLAYLLKLE
WSSLSSLSIKTHSLPLTSLVARCIDLQMTNEPGCDPQLVAKIGGNSDVIKNQLLRYLD
TDTLLVFSPMNEFEGRLRNAQNELYIPIIKGMEEFLRNFSSESNIRLQILDADIHGLR
GNQQSDIVKNAAKKYMSSLSPWDLAILEKTVLTTKSFICGVLLLENKKDTANLIPALK
TDMDNIVRAATLETIFQVEKWGEVEDTHDVDKRDIRRKIHTAAIAAFKQ"
gene <88787..>89116
/gene="PFD1"
/locus_tag="YJL179W"
/gene_synonym="GIM6"
/db_xref="GeneID:853260"
mRNA <88787..>89116
/gene="PFD1"
/locus_tag="YJL179W"
/gene_synonym="GIM6"
/product="prefolding complex chaperone subunit"
/transcript_id="NM_001181612.2"
/db_xref="GeneID:853260"
CDS 88787..89116
/gene="PFD1"
/locus_tag="YJL179W"
/gene_synonym="GIM6"
/experiment="EXISTENCE:genetic interaction:GO:0032968
positive regulation of transcription elongation by RNA
polymerase II [PMID:24068951]"
/experiment="EXISTENCE:mutant phenotype:GO:0006457 protein
folding [PMID:9630229]"
/experiment="EXISTENCE:mutant phenotype:GO:0007010
cytoskeleton organization [PMID:9630229]"
/experiment="EXISTENCE:mutant phenotype:GO:0016272
prefoldin complex [PMID:9630229]"
/experiment="EXISTENCE:mutant phenotype:GO:0032968
positive regulation of transcription elongation by RNA
polymerase II [PMID:24068951]"
/experiment="EXISTENCE:mutant phenotype:GO:0051082
unfolded protein binding [PMID:9630229]"
/note="Subunit of heterohexameric prefoldin; prefoldin
binds cytosolic chaperonin and transfers target proteins
to it; involved in the biogenesis of actin and of alpha-
and gamma-tubulin; prefoldin complex also localizes to
chromatin of actively transcribed genes in the nucleus and
facilitates transcriptional elongation"
/codon_start=1
/product="prefolding complex chaperone subunit"
/protein_id="NP_012356.2"
/db_xref="GeneID:853260"
/db_xref="SGD:S000003715"
/translation="MSQIAQEMTVSLRNARTQLDMVNQQLAYLDRQEKLAELTKKELE
SYPTDKVWRSCGKSFILQDKSKYVNDLSHDETVLLDQRKTLKIKKNYLETTVEKTIDN
LKALMKN"
gene complement(<89282..>90097)
/gene="ATG27"
/locus_tag="YJL178C"
/gene_synonym="ETF1"
/db_xref="GeneID:853261"
mRNA complement(<89282..>90097)
/gene="ATG27"
/locus_tag="YJL178C"
/gene_synonym="ETF1"
/product="Atg27p"
/transcript_id="NM_001181611.1"
/db_xref="GeneID:853261"
CDS complement(89282..90097)
/gene="ATG27"
/locus_tag="YJL178C"
/gene_synonym="ETF1"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0000407 phagophore
assembly site [PMID:17135291]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:17135291]"
/experiment="EXISTENCE:direct assay:GO:0005774 vacuolar
membrane [PMID:25557545]"
/experiment="EXISTENCE:direct assay:GO:0005802 trans-Golgi
network [PMID:16107716]"
/experiment="EXISTENCE:direct assay:GO:0016236
macroautophagy [PMID:25557545]"
/experiment="EXISTENCE:direct assay:GO:0030136
clathrin-coated vesicle [PMID:33817564]"
/experiment="EXISTENCE:direct assay:GO:0032266
phosphatidylinositol-3-phosphate binding [PMID:12186856]"
/experiment="EXISTENCE:mutant phenotype:GO:0000045
autophagosome assembly [PMID:17135291]"
/experiment="EXISTENCE:mutant phenotype:GO:0000425
pexophagy [PMID:17135291]"
/experiment="EXISTENCE:mutant phenotype:GO:0016050 vesicle
organization [PMID:12186856]"
/experiment="EXISTENCE:mutant phenotype:GO:0032258
cytoplasm to vacuole targeting by the Cvt pathway
[PMID:17135291]"
/experiment="EXISTENCE:mutant phenotype:GO:0034497 protein
localization to phagophore assembly site [PMID:17135291]"
/note="Type I membrane protein involved in autophagy and
the Cvt pathway; targeted to vacuole via AP-3 pathway"
/codon_start=1
/product="Atg27p"
/protein_id="NP_012357.2"
/db_xref="GeneID:853261"
/db_xref="SGD:S000003714"
/translation="MVSKTWICGFISIITVVQALSCEKHDVLKKYQVGKFSSLTSTER
DTPPSTTIEKWWINVCEEHNVEPPEECKKNDMLCGLTDVILPGKDAITTQIIDFDKNI
GFNVEETESALTLTLKGATWGANSFDAKLEFQCNDNMKQDELTSHTWADKSIQLTLKG
PSGCLKSKDDDKKNGDGDNGKDGDSEGKKPAKKAGGTSWFTWLFLYALLFTLIYLMVV
SFLNTRGGSFQDFRAEFIQRSTQFLTSLPEFCKEVVSRILGRSTAQRGGYSAV"
gene <90786..>91657
/gene="RPL17B"
/locus_tag="YJL177W"
/db_xref="GeneID:853262"
mRNA join(<90786..91094,91412..>91657)
/gene="RPL17B"
/locus_tag="YJL177W"
/product="60S ribosomal protein uL22 RPL17B"
/transcript_id="NM_001181610.1"
/db_xref="GeneID:853262"
CDS join(90786..91094,91412..91657)
/gene="RPL17B"
/locus_tag="YJL177W"
/experiment="EXISTENCE:direct assay:GO:0002181 cytoplasmic
translation [PMID:18782943]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:18782943]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:18782943]"
/experiment="EXISTENCE:direct assay:GO:0030687
preribosome, large subunit precursor [PMID:23268442]"
/experiment="EXISTENCE:genetic interaction:GO:0000448
cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of
tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA,
LSU-rRNA) [PMID:23268442]"
/note="Ribosomal 60S subunit protein L17B; required for
processing of 27SB pre-rRNA and formation of stable 66S
assembly intermediates; homologous to mammalian ribosomal
protein L17 and bacterial L22; RPL17B has a paralog,
RPL17A, that arose from the whole genome duplication"
/codon_start=1
/product="60S ribosomal protein uL22 RPL17B"
/protein_id="NP_012358.1"
/db_xref="GeneID:853262"
/db_xref="SGD:S000003713"
/translation="MARYGATSTNPAKSASARGSYLRVSFKNTRETAQAINGWELTKA
QKYLDQVLDHQRAIPFRRFNSSIGRTAQGKEFGVTKARWPAKSVKFVQGLLQNAAANA
EAKGLDATKLYVSHIQVNQAPKQRRRTYRAHGRINKYESSPSHIELVVTEKEEAVAKA
AEKKVVRLTSRQRGRIAAQKRISA"
gene complement(<92053..>94530)
/gene="SWI3"
/locus_tag="YJL176C"
/gene_synonym="HAF2; TYE2"
/db_xref="GeneID:853264"
mRNA complement(<92053..>94530)
/gene="SWI3"
/locus_tag="YJL176C"
/gene_synonym="HAF2; TYE2"
/product="Swi3p"
/transcript_id="NM_001181609.1"
/db_xref="GeneID:853264"
CDS complement(92053..94530)
/gene="SWI3"
/locus_tag="YJL176C"
/gene_synonym="HAF2; TYE2"
/experiment="EXISTENCE:direct assay:GO:0003677 DNA binding
[PMID:16461455]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:1339306|PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0006338 chromatin
remodeling [PMID:8016655]"
/experiment="EXISTENCE:direct assay:GO:0016514 SWI/SNF
complex
[PMID:8016655|PMID:8159677|PMID:8127913|PMID:18644858]"
/experiment="EXISTENCE:mutant phenotype:GO:0003677 DNA
binding [PMID:16461455]"
/experiment="EXISTENCE:mutant phenotype:GO:0016514 SWI/SNF
complex [PMID:8159677]"
/experiment="EXISTENCE:mutant phenotype:GO:0031496
positive regulation of mating type switching
[PMID:6436497]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:1339306|PMID:3542227]"
/experiment="EXISTENCE:physical interaction:GO:0042393
histone binding [PMID:17496903]"
/note="Subunit of the SWI/SNF chromatin remodeling
complex; SWI/SNF regulates transcription by remodeling
chromosomes; contains SANT domain that is required for
SWI/SNF assembly; is essential for displacement of histone
H2A-H2B dimers during ATP-dependent remodeling; required
for transcription of many genes, including ADH1, ADH2,
GAL1, HO, INO1 and SUC2; relocates to the cytosol under
hypoxic conditions"
/codon_start=1
/product="Swi3p"
/protein_id="NP_012359.1"
/db_xref="GeneID:853264"
/db_xref="SGD:S000003712"
/translation="MENTLGEGSTVNASVDVDQHGNDNNSDSNANAAVAGVANTDTAG
EESQQQDESLKDEATVPNTRDAESEAITVTAKQQPTMQANKLDSQETPSTEESRAQNV
FGQDNEDSDNLFGETESSVSNNEANTPSIPTNPVDNENNKPAIKEDSTIQDSNGDVKN
MEDVKIQKEEEPENNTVIEGVKEESQPDENTKEMDEVEEDDEDDDQPMISPDNSIFGD
TKSESKQLGNTSSVANTPSEIPDAHKAEQEDIIEKTESVDKKVDSGEERNEQEREIMN
DHSKSANPKKTTITRVEPETFEIPQAHEIVIPSYSKWFNLEKIHSIEVQSLPEFFTNR
IPSKTPEVYMRYRNFMVNSYRLNPNEYFSVTTARRNVSGDAAALFRLHKFLTKWGLIN
YQVDSKLLPKNIEPPLTSQYSTRHDAPRGLFPFESYKPSVQLPDMAKLKKMMNTSDSE
STLYKYLKESKRKYDEITHPPSTTDDENGDKNDNGGKMNNEVSTSTSMTGDANLLEEG
ETSRPLKKVKILEQIDENWSKEDLQKLLKGIQEFGADWYKVAKNVGNKSPEQCILRFL
QLPIEDKFLYGDGNGKGDNDNGLGPLKYAPHLPFSKSENPVLSTIAFLVGLVNPKTVQ
SMTQRAIQSAESIKSQKEEISDQKPIEHIKEGSEIAISSLGYRSHIFATNEERQMNFL
TNELIRLQMEKLDAKLNHLKKLEKFMELERKTLERQQENLLIQRLNFNQNSSKIVNVL
SKCLNLISDSNINNSSVAEKEEIRSQIDHFKSMLSKPETLSIGKNPFNKPNIETGENH
NGQSISNENDVKPISIEAPQFYRYWSA"
gene <95092..>95922
/gene="KRE9"
/locus_tag="YJL174W"
/db_xref="GeneID:853265"
mRNA <95092..>95922
/gene="KRE9"
/locus_tag="YJL174W"
/product="Kre9p"
/transcript_id="NM_001181607.1"
/db_xref="GeneID:853265"
CDS 95092..95922
/gene="KRE9"
/locus_tag="YJL174W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005576
extracellular region [PMID:8413233]"
/experiment="EXISTENCE:mutant phenotype:GO:0006077
(1->6)-beta-D-glucan metabolic process [PMID:8413233]"
/experiment="EXISTENCE:mutant phenotype:GO:0031505
fungal-type cell wall organization [PMID:8413233]"
/note="Glycoprotein involved in cell wall beta-glucan
assembly; null mutation leads to severe growth defects,
aberrant multibudded morphology, and mating defects"
/codon_start=1
/product="Kre9p"
/protein_id="NP_012361.1"
/db_xref="GeneID:853265"
/db_xref="SGD:S000003710"
/translation="MRLQRNSIICALVFLVSFVLGDVNIVSPSSKATFSPSGGTVSVP
VEWMDNGAYPSLSKISTFTFSLCTGPNNNIDCVAVLASKITPSELTQDDKVYSYTAEF
ASTLTGNGQYYIQVFAQVDGQGYTIHYTPRFQLTSMGGVTAYTYSATTEPTPQTSIQT
TTTNNAQATTIDSRSFTVPYTKQTGTSRFAPMQMQPNTKVTATTWTRKFATSAVTYYS
TFGSLPEQATTITPGWSYTISSGVNYATPASMPSDNGGWYKPSKRLSLSARKINMRKV
"
gene complement(<96161..>96529)
/gene="RFA3"
/locus_tag="YJL173C"
/gene_synonym="RPA14; RPA3"
/db_xref="GeneID:853266"
mRNA complement(<96161..>96529)
/gene="RFA3"
/locus_tag="YJL173C"
/gene_synonym="RPA14; RPA3"
/product="Rfa3p"
/transcript_id="NM_001181606.1"
/db_xref="GeneID:853266"
CDS complement(96161..96529)
/gene="RFA3"
/locus_tag="YJL173C"
/gene_synonym="RPA14; RPA3"
/experiment="EXISTENCE:direct assay:GO:0000794 condensed
nuclear chromosome [PMID:9679065]"
/experiment="EXISTENCE:direct assay:GO:0003690
double-stranded DNA binding [PMID:7761422]"
/experiment="EXISTENCE:direct assay:GO:0005662 DNA
replication factor A complex [PMID:2554144]"
/experiment="EXISTENCE:direct assay:GO:0006260 DNA
replication [PMID:2554144]"
/experiment="EXISTENCE:direct assay:GO:0006265 DNA
topological change [PMID:22885009]"
/experiment="EXISTENCE:direct assay:GO:0006289
nucleotide-excision repair [PMID:8910442]"
/experiment="EXISTENCE:direct assay:GO:0030491
heteroduplex formation [PMID:12226081]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:7761422]"
/experiment="EXISTENCE:genetic interaction:GO:0000724
double-strand break repair via homologous recombination
[PMID:9679065]"
/experiment="EXISTENCE:mutant phenotype:GO:0000781
chromosome, telomeric region [PMID:14702040]"
/experiment="EXISTENCE:mutant phenotype:GO:0006260 DNA
replication [PMID:9093844]"
/experiment="EXISTENCE:mutant phenotype:GO:0006281 DNA
repair [PMID:9093844]"
/experiment="EXISTENCE:mutant phenotype:GO:0010833
telomere maintenance via telomere lengthening
[PMID:22354040]"
/experiment="EXISTENCE:physical interaction:GO:0000722
telomere maintenance via recombination [PMID:2554144]"
/experiment="EXISTENCE:physical interaction:GO:0006312
mitotic recombination [PMID:2554144]"
/experiment="EXISTENCE:physical interaction:GO:0007004
telomere maintenance via telomerase [PMID:2554144]"
/experiment="EXISTENCE:physical interaction:GO:0007131
reciprocal meiotic recombination [PMID:2554144]"
/experiment="EXISTENCE:physical interaction:GO:0016567
protein ubiquitination [PMID:18342608]"
/experiment="EXISTENCE:physical interaction:GO:0045184
establishment of protein localization [PMID:2554144]"
/note="Subunit of heterotrimeric Replication Protein A
(RPA); RPA is a highly conserved single-stranded DNA
binding protein complex involved in DNA replication,
repair, recombination; RPA protects against inappropriate
telomere recombination, and upon telomere uncapping,
prevents cell proliferation by a checkpoint-independent
pathway; with Sgs1p-Top2p-Rmi1p, stimulates DNA
catenation/decatenation activity of Top3p; protein
abundance increases in response to DNA replication stress"
/codon_start=1
/product="Rfa3p"
/protein_id="NP_012362.1"
/db_xref="GeneID:853266"
/db_xref="SGD:S000003709"
/translation="MASETPRVDPTEISNVNAPVFRIIAQIKSQPTESQLILQSPTIS
SKNGSEVEMITLNNIRVSMNKTFEIDSWYEFVCRNNDDGELGFLILDAVLCKFKENED
LSLNGVVALQRLCKKYPEIY"
gene <97737..>99467
/gene="CPS1"
/locus_tag="YJL172W"
/db_xref="GeneID:853267"
mRNA <97737..>99467
/gene="CPS1"
/locus_tag="YJL172W"
/product="Gly-Xaa carboxypeptidase"
/transcript_id="NM_001181605.1"
/db_xref="GeneID:853267"
CDS 97737..99467
/gene="CPS1"
/locus_tag="YJL172W"
/EC_number="3.4.17.4"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0000328 fungal-type
vacuole lumen [PMID:1569061]"
/experiment="EXISTENCE:mutant phenotype:GO:0004180
carboxypeptidase activity [PMID:2026161]"
/experiment="EXISTENCE:mutant phenotype:GO:0008152
metabolic process [PMID:2026161]"
/experiment="EXISTENCE:mutant phenotype:GO:0051603
proteolysis involved in protein catabolic process
[PMID:2026161]"
/note="Vacuolar carboxypeptidase S; targeted to vacuole
via Vps10p-dependent endosomal vacuolar protein sorting
pathway and via AP-3 pathway; expression induced under
low-nitrogen conditions"
/codon_start=1
/product="Gly-Xaa carboxypeptidase"
/protein_id="NP_012363.1"
/db_xref="GeneID:853267"
/db_xref="SGD:S000003708"
/translation="MIALPVEKAPRKSLWQRHRAFISGIVALIIIGTFFLTSGLHPAP
PHEAKRPHHGKGPMHSPKCEKIEPLSPSFKHSVDTILHDPAFRNSSIEKLSNAVRIPT
VVQDKNPNPADDPDFYKHFYELHDYFEKTFPNIHKHLKLEKVNELGLLYTWEGSDPDL
KPLLLMAHQDVVPVNNETLSSWKFPPFSGHYDPETDFVWGRGSNDCKNLLIAEFEAIE
QLLIDGFKPNRTIVMSLGFDEEASGTLGAASLASFLHERYGDDGIYSIIDEGEGIMEV
DKDVFVATPINAEKGYVDFEVSILGHGGHSSVPPDHTTIGIASELITEFEANPFDYEF
EFDNPIYGLLTCAAEHSKSLSKDVKKTILGAPFCPRRKDKLVEYISNQSHLRSLIRTT
QAVDIINGGVKANALPETTRFLINHRINLHSSVAEVFERNIEYAKKIAEKYGYGLSKN
GDDYIIPETELGHIDITLLRELEPAPLSPSSGPVWDILAGTIQDVFENGVLQNNEEFY
VTTGLFSGNTDTKYYWNLSKNIYRFVGSIIDIDLLKTLHSVNEHVDVPGHLSAIAFVY
EYIVNVNEYA"
rep_origin 99458..99678
/note="ARS1006; Autonomously Replicating Sequence"
/db_xref="SGD:S000028443"
gene complement(<99704..>100894)
/gene="TOH1"
/locus_tag="YJL171C"
/db_xref="GeneID:853268"
mRNA complement(<99704..>100894)
/gene="TOH1"
/locus_tag="YJL171C"
/product="Toh1p"
/transcript_id="NM_001181604.2"
/db_xref="GeneID:853268"
CDS complement(99704..100894)
/gene="TOH1"
/locus_tag="YJL171C"
/EC_number="3.2.1.39"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:10383953]"
/note="GPI-anchored cell wall hypothetical protein;
induced in response to cell wall damaging agents and by
mutations in genes involved in cell wall biogenesis;
sequence similarity to YBR162C/TOS1, a covalently bound
cell wall protein; protein abundance increases in response
to DNA replication stress"
/codon_start=1
/product="Toh1p"
/protein_id="NP_012364.2"
/db_xref="GeneID:853268"
/db_xref="SGD:S000003707"
/translation="MLQSIVLSVCMFMLHTVAASGPQSYQKLDFTNVGFTGSYVDVNK
FKDITNNESCTCEVGDRVWFSGKNAPLADYLSVHFRGPLKLKQFAFYTSPGFTVNNSR
SSSDWNRLAYYESSSKTADNVTFLNHGGEASPCLGNALSYASSNGTGSASEATVLADG
TLISSDQEYIIYSNVSCPKSGYDKGCGVYRSGIPAYYGYGGTTKMFLFEFEMPTETEK
NSSSIGYYDLPAIWLLNDHIARTSQYPTNANCSCWASGCGEYDIFEAMNGTEKNHLYS
TFHTFQGIEDLGTGIQSYGYITRNTTGTMKGGVVFDSSGNVVSFISDATPFNGTVSAD
TVNDLLAAIPENETYSSQLMSISATAPSTTSKSNGVALTKMQNGVWYYILAIFTAFTQ
VVLI"
gene complement(<101150..>101779)
/gene="ASG7"
/locus_tag="YJL170C"
/db_xref="GeneID:853269"
mRNA complement(<101150..>101779)
/gene="ASG7"
/locus_tag="YJL170C"
/product="Asg7p"
/transcript_id="NM_001181603.1"
/db_xref="GeneID:853269"
CDS complement(101150..101779)
/gene="ASG7"
/locus_tag="YJL170C"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:11073983]"
/experiment="EXISTENCE:expression pattern:GO:0000747
conjugation with cellular fusion [PMID:10568744]"
/note="Protein that regulates signaling from G protein
beta subunit Ste4p; contributes to relocalization of Ste4p
within the cell; specific to a-cells and induced by
alpha-factor"
/codon_start=1
/product="Asg7p"
/protein_id="NP_012365.2"
/db_xref="GeneID:853269"
/db_xref="SGD:S000003706"
/translation="MTTLASSIEHKTKHLAAPFENDENPWMKKYCCQCKSCKMSVPVQ
PWLPRFFVFGILCPVFWLVNLLAWWFLQYWQPHELEFHDLQEDEYPGFYEYEAITKRT
VIPIKEEVLQEIRVMQNFSDSNSEEYYESKDGMPSSFLNVNTEQVEDENDTLKKYRYA
FLKKVAHDVLESHDLLRKTFRDWNLRSLLGLLIDSILIIFVVLLCKKSR"
gene complement(<102227..>104428)
/gene="SET2"
/locus_tag="YJL168C"
/gene_synonym="EZL1; KMT3"
/db_xref="GeneID:853271"
mRNA complement(<102227..>104428)
/gene="SET2"
/locus_tag="YJL168C"
/gene_synonym="EZL1; KMT3"
/product="histone methyltransferase SET2"
/transcript_id="NM_001181601.2"
/db_xref="GeneID:853271"
CDS complement(102227..104428)
/gene="SET2"
/locus_tag="YJL168C"
/gene_synonym="EZL1; KMT3"
/EC_number="2.1.1.359"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:28483910]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0006354
DNA-templated transcription elongation [PMID:12736296]"
/experiment="EXISTENCE:direct assay:GO:0006355 regulation
of DNA-templated transcription [PMID:11839797]"
/experiment="EXISTENCE:direct assay:GO:0046975 histone
H3K36 methyltransferase activity [PMID:11839797]"
/experiment="EXISTENCE:mutant phenotype:GO:0006283
transcription-coupled nucleotide-excision repair
[PMID:35263330]"
/experiment="EXISTENCE:mutant phenotype:GO:0006353
DNA-templated transcription termination [PMID:23109428]"
/experiment="EXISTENCE:mutant phenotype:GO:0006355
regulation of DNA-templated transcription [PMID:11839797]"
/experiment="EXISTENCE:mutant phenotype:GO:0009302
sno(s)RNA transcription [PMID:23109428]"
/experiment="EXISTENCE:mutant phenotype:GO:0030174
regulation of DNA-templated DNA replication initiation
[PMID:19417103]"
/experiment="EXISTENCE:mutant phenotype:GO:0030437
ascospore formation [PMID:17150765]"
/experiment="EXISTENCE:mutant phenotype:GO:0045128
negative regulation of reciprocal meiotic recombination
[PMID:18515193]"
/experiment="EXISTENCE:mutant phenotype:GO:0046975 histone
H3K36 methyltransferase activity [PMID:11839797]"
/experiment="EXISTENCE:mutant phenotype:GO:0060195
negative regulation of antisense RNA transcription
[PMID:21248844]"
/note="Histone methyltransferase with a role in
transcriptional elongation; methylates H3 lysine 36
(H3K36), which suppresses incorporation of acetylated
histones and signals for their deacetylation within
transcribed genes; involved in transcription
coupled-nucleotide excision repair; associates with
C-terminal domain (CTD) of Rpo21p; H3K36me3
(trimethylation) requires Spt6p, proline 38 on H3, CTD of
Rpo21p, Ctk1p, and C-terminal SRI domain of Ste2p;
relocalizes to cytosol in response to hypoxia"
/codon_start=1
/product="histone methyltransferase SET2"
/protein_id="NP_012367.2"
/db_xref="GeneID:853271"
/db_xref="SGD:S000003704"
/translation="MSKNQSVSASEDEKEILNNNAEGHKPQRLFDQEPDLTEEALTKF
ENLDDCIYANKRIGTFKNNDFMECDCYEEFSDGVNHACDEDSDCINRLTLIECVNDLC
SSCGNDCQNQRFQKKQYAPIAIFKTKHKGYGVRAEQDIEANQFIYEYKGEVIEEMEFR
DRLIDYDQRHFKHFYFMMLQNGEFIDATIKGSLARFCNHSCSPNAYVNKWVVKDKLRM
GIFAQRKILKGEEITFDYNVDRYGAQAQKCYCEEPNCIGFLGGKTQTDAASLLPQNIA
DALGVTVSMEKKWLKLKKLSGEPIIKNENENINIEFLQSLEVQPIDSPVDVTKIMSVL
LQQDNKIIASKLLKRLFTIDDDSLRHQAIKLHGYTCFSKMLKLFITEQPQVDGKGNET
EEDDIKFIKGILDFLLELPKTTRNGIESSQIDNVVKTLPAKFPFLKPNCDELLEKWSK
FETYKRITKKDINVAASKMIDLRRVRLPPGWEIIHENGRPLYYNAEQKTKLHYPPSGS
SKVFSSRSNTQVNSPSSSGIPKTPGALDSKKHKLSDEEYERKKQKRLEYERIALERAK
QEELESLKQKLKLENERKSVLEDIIAEANKQKELQKEEAKKLVEAKEAKRLKRKTVSQ
SQRLEHNWNKFFASFVPNLIKKNPQSKQFDHENIKQCAKDIVKILTTKELKKDSSRAP
PDDLTKGKRHKVKEFINSYMDKIILKKKQKKALALSSASTRMSSPPPSTSS"
gene <105014..>106072
/gene="ERG20"
/locus_tag="YJL167W"
/gene_synonym="BOT3; FDS1; FPP1"
/db_xref="GeneID:853272"
mRNA <105014..>106072
/gene="ERG20"
/locus_tag="YJL167W"
/gene_synonym="BOT3; FDS1; FPP1"
/product="bifunctional (2E,6E)-farnesyl diphosphate
synthase/dimethylallyltranstransferase"
/transcript_id="NM_001181600.1"
/db_xref="GeneID:853272"
CDS 105014..106072
/gene="ERG20"
/locus_tag="YJL167W"
/gene_synonym="BOT3; FDS1; FPP1"
/EC_number="2.5.1.1"
/EC_number="2.5.1.10"
/experiment="EXISTENCE:direct assay:GO:0004161
dimethylallyltranstransferase activity [PMID:2681213]"
/experiment="EXISTENCE:direct assay:GO:0004337
(2E,6E)-farnesyl diphosphate synthase activity
[PMID:2681213]"
/experiment="EXISTENCE:direct assay:GO:0004452
isopentenyl-diphosphate delta-isomerase activity
[PMID:7908830]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:19416104]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:19416104]"
/experiment="EXISTENCE:direct assay:GO:0006696 ergosterol
biosynthetic process [PMID:7908830]"
/experiment="EXISTENCE:direct assay:GO:0008299 isoprenoid
biosynthetic process [PMID:2681213]"
/experiment="EXISTENCE:direct assay:GO:0045337 farnesyl
diphosphate biosynthetic process [PMID:2681213]"
/experiment="EXISTENCE:mutant phenotype:GO:0006696
ergosterol biosynthetic process [PMID:1779710]"
/note="Farnesyl pyrophosphate synthetase; has both
dimethylallyltranstransferase and geranyltranstransferase
activities; catalyzes the formation of C15 farnesyl
pyrophosphate units for isoprenoid and sterol
biosynthesis"
/codon_start=1
/product="bifunctional (2E,6E)-farnesyl diphosphate
synthase/dimethylallyltranstransferase"
/protein_id="NP_012368.1"
/db_xref="GeneID:853272"
/db_xref="SGD:S000003703"
/translation="MASEKEIRRERFLNVFPKLVEELNASLLAYGMPKEACDWYAHSL
NYNTPGGKLNRGLSVVDTYAILSNKTVEQLGQEEYEKVAILGWCIELLQAYFLVADDM
MDKSITRRGQPCWYKVPEVGEIAINDAFMLEAAIYKLLKSHFRNEKYYIDITELFHEV
TFQTELGQLMDLITAPEDKVDLSKFSLKKHSFIVTFKTAYYSFYLPVALAMYVAGITD
EKDLKQARDVLIPLGEYFQIQDDYLDCFGTPEQIGKIGTDIQDNKCSWVINKALELAS
AEQRKTLDENYGKKDSVAEAKCKKIFNDLKIEQLYHEYEESIAKDLKAKISQVDESRG
FKADVLTAFLNKVYKRSK"
gene <106434..>106718
/gene="QCR8"
/locus_tag="YJL166W"
/gene_synonym="COR5"
/db_xref="GeneID:853273"
mRNA <106434..>106718
/gene="QCR8"
/locus_tag="YJL166W"
/gene_synonym="COR5"
/product="ubiquinol--cytochrome-c reductase subunit 8"
/transcript_id="NM_001181599.1"
/db_xref="GeneID:853273"
CDS 106434..106718
/gene="QCR8"
/locus_tag="YJL166W"
/gene_synonym="COR5"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0045275 respiratory
chain complex III [PMID:10873857]"
/experiment="EXISTENCE:mutant phenotype:GO:0006122
mitochondrial electron transport, ubiquinol to cytochrome
c [PMID:8394810]"
/experiment="EXISTENCE:mutant phenotype:GO:0008121
quinol-cytochrome-c reductase activity [PMID:8786239]"
/experiment="EXISTENCE:mutant phenotype:GO:0009060 aerobic
respiration [PMID:8394810]"
/note="Subunit 8 of ubiquinol cytochrome-c reductase
(Complex III); Complex III is a component of the
mitochondrial inner membrane electron transport chain;
oriented facing the intermembrane space; expression is
regulated by Abf1p and Cpf1p"
/codon_start=1
/product="ubiquinol--cytochrome-c reductase subunit 8"
/protein_id="NP_012369.1"
/db_xref="GeneID:853273"
/db_xref="SGD:S000003702"
/translation="MGPPSGKTYMGWWGHMGGPKQKGITSYAVSPYAQKPLQGIFHNA
VFNSFRRFKSQFLYVLIPAGIYWYWWKNGNEYNEFLYSKAGREELERVNV"
gene complement(<106894..>109461)
/gene="HAL5"
/locus_tag="YJL165C"
/db_xref="GeneID:853274"
mRNA complement(<106894..>109461)
/gene="HAL5"
/locus_tag="YJL165C"
/product="protein kinase HAL5"
/transcript_id="NM_001181598.1"
/db_xref="GeneID:853274"
CDS complement(106894..109461)
/gene="HAL5"
/locus_tag="YJL165C"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:32191698]"
/experiment="EXISTENCE:genetic interaction:GO:0008104
intracellular protein localization [PMID:21237705]"
/experiment="EXISTENCE:mutant phenotype:GO:0030003
intracellular monoatomic cation homeostasis
[PMID:10207057]"
/experiment="EXISTENCE:mutant phenotype:GO:0045807
positive regulation of endocytosis [PMID:32191698]"
/note="Snf1p-related nutrient-responsive protein kinase;
overexpression increases sodium and lithium tolerance,
whereas gene disruption increases cation and low pH
sensitivity and impairs potassium uptake, suggesting a
role in regulation of Trk1p and/or Trk2p transporters;
HAL5 has a paralog, KKQ8, that arose from the whole genome
duplication"
/codon_start=1
/product="protein kinase HAL5"
/protein_id="NP_012370.1"
/db_xref="GeneID:853274"
/db_xref="SGD:S000003701"
/translation="MGDEKLSRHTSLKRARSLSESIKGLFKPSGISGSNNAAAPSSRP
GQDQAHSHQTARIITSNVSSPSISPVHSPVLQAAPKHHKLGVPNIAKLSLSPSREPSL
NSENEMFSQESFISEKDEDEANLLEREDLQNKKEEKARAKHVRSKEAYVPHHRYTVGS
DEVERQPRERLKNFPQNAGSSNPANSNANHVLDQENNFSIDAMLDYDEESKLRRRNSL
GVRNHSNRTRSRKNSLSTPRSPPMKNGNGGMNSNATNNVGNGTGNRIYMRGRNHSDSI
SASSLPKFQEIECKCILDLGHFKVFENGYHEHSLRVLPIITNNKNVDSGDEKDADASV
NSGDDGDNDSEANMHKQKSVFSLSGLFKSHKDGNQQQQQQQQQEENGEQINLEKAFSI
IPSQRFIKSQTLKKSRTSNLKNGNNDELMKNDGKNIPQIVNPNAAVGVEELKLINALS
EKIRKGLKSENTKGNNGEGRSNSNKQEDSDDTEGKAGTTNDDTSHKPCSQKYGKSIGV
VGAGAYGVVKICARCKTAKDVLPYSTYSNGKKLFFAVKELKPKPGDQIDKFCTRLTSE
FIIGHSLSHPHFEANAMIAGNVSRTTPPKHVFNAPNILKILDLMEYSNSFVEVMEFCA
SGDLYSLLTRNNISNESNNGSSRLIQTVKEGSGSPLHPLEADCFMKQLLNGVQYMHDH
GIAHCDLKPENILFQPNGLLKICDFGTSSVFQTAWEKHVHFQSGAMGSEPYVAPEEFI
RDAEYDPRLVDCWSCGIVYCTMVMGQYLWKIAIPEKDSLFKSFLSEIKDDGQFYLFEE
LRHVSSELNRLRKIALYRTFQVDPTKRITIEQLLQSSWMRKTKCCVVYRHLHTKVSK"
gene complement(<109966..>111159)
/gene="TPK1"
/locus_tag="YJL164C"
/gene_synonym="PKA1; SRA3"
/db_xref="GeneID:853275"
mRNA complement(<109966..>111159)
/gene="TPK1"
/locus_tag="YJL164C"
/gene_synonym="PKA1; SRA3"
/product="cAMP-dependent protein kinase catalytic subunit
TPK1"
/transcript_id="NM_001181597.2"
/db_xref="GeneID:853275"
CDS complement(109966..111159)
/gene="TPK1"
/locus_tag="YJL164C"
/gene_synonym="PKA1; SRA3"
/EC_number="2.7.11.11"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:26403272]"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0004691
cAMP-dependent protein kinase activity
[PMID:34244791|PMID:3036373]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10625697]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:10625697]"
/experiment="EXISTENCE:direct assay:GO:0005952
cAMP-dependent protein kinase complex [PMID:12024012]"
/experiment="EXISTENCE:direct assay:GO:0018105
peptidyl-serine phosphorylation [PMID:34244791]"
/experiment="EXISTENCE:genetic interaction:GO:0004691
cAMP-dependent protein kinase activity [PMID:3036373]"
/experiment="EXISTENCE:genetic interaction:GO:0005952
cAMP-dependent protein kinase complex [PMID:3036373]"
/experiment="EXISTENCE:genetic interaction:GO:0006303
double-strand break repair via nonhomologous end joining
[PMID:34244791|PMID:33008596]"
/experiment="EXISTENCE:genetic interaction:GO:0007265 Ras
protein signal transduction [PMID:3036373]"
/experiment="EXISTENCE:genetic interaction:GO:0010607
negative regulation of cytoplasmic mRNA processing body
assembly [PMID:21925385]"
/experiment="EXISTENCE:mutant phenotype:GO:0004691
cAMP-dependent protein kinase activity [PMID:3036373]"
/experiment="EXISTENCE:mutant phenotype:GO:0006303
double-strand break repair via nonhomologous end joining
[PMID:34244791|PMID:33008596]"
/experiment="EXISTENCE:mutant phenotype:GO:0006606 protein
import into nucleus [PMID:34244791]"
/experiment="EXISTENCE:mutant phenotype:GO:0007265 Ras
protein signal transduction [PMID:2823100]"
/experiment="EXISTENCE:mutant phenotype:GO:0016241
regulation of macroautophagy [PMID:15016820]"
/experiment="EXISTENCE:mutant phenotype:GO:0018105
peptidyl-serine phosphorylation [PMID:34244791]"
/experiment="EXISTENCE:physical interaction:GO:0005952
cAMP-dependent protein kinase complex [PMID:12024012]"
/note="cAMP-dependent protein kinase catalytic subunit;
role in nutrient-stimulated growth via Ras-cAMP signaling;
inhibited by regulatory subunit Bcy1p in cAMPs absence;
phosphorylates and inhibits Whi3p to promote G1/S phase
passage; phosphorylates Nej1p to regulate nonhomologous
end joining (NHEJ); impairs mitochondrial import of
pre-Tom40p via phosphorylation under non-respiratory
conditions; partially redundant with Tpk2p and Tpk3p;
human homolog PRKACB regulates NHEJ via NHEJ1
phosphorylation"
/codon_start=1
/product="cAMP-dependent protein kinase catalytic subunit
TPK1"
/protein_id="NP_012371.2"
/db_xref="GeneID:853275"
/db_xref="SGD:S000003700"
/translation="MSTEEQNGGGQKSLDDRQGEESQKGETSERETTATESGNESKSV
EKEGGETQEKPKQPHVTYYNEEQYKQFIAQARVTSGKYSLQDFQILRTLGTGSFGRVH
LIRSRHNGRYYAMKVLKKEIVVRLKQVEHTNDERLMLSIVTHPFIIRMWGTFQDAQQI
FMIMDYIEGGELFSLLRKSQRFPNPVAKFYAAEVCLALEYLHSKDIIYRDLKPENILL
DKNGHIKITDFGFAKYVPDVTYTLCGTPDYIAPEVVSTKPYNKSIDWWSFGILIYEML
AGYTPFYDSNTMKTYEKILNAELRFPPFFNEDVKDLLSRLITRDLSQRLGNLQNGTED
VKNHPWFKEVVWEKLLSRNIETPYEPPIQQGQGDTSQFDKYPEEDINYGVQGEDPYAD
LFRDF"
gene complement(<111666..>113333)
/locus_tag="YJL163C"
/db_xref="GeneID:853276"
mRNA complement(<111666..>113333)
/locus_tag="YJL163C"
/product="uncharacterized protein"
/transcript_id="NM_001181596.1"
/db_xref="GeneID:853276"
CDS complement(111666..113333)
/locus_tag="YJL163C"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762]"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_012372.1"
/db_xref="GeneID:853276"
/db_xref="SGD:S000003699"
/translation="MSNEDETTRLMSSDEMDYLLETAGINALEEIISQNDSTGINLDT
NETAQDSSYDSIRRSPSILSVAKSVEGEHGRRKLLCLYGLVMIICIAESISMTATIPL
VMDKVAEGISDENGHYDSVAVQTIVSSISSSTMMIAGAISIFMAGKWGELSDRIGRVR
VFKYMSGIRVIGLLTHVFTLSSKMKYHKWAIVLTACIVPSFGGLFALVANGNSYVSDI
VKTEHRMVTIGIMMSCIYATMGVGPMFGSFLVKWTHGNGFIPIYTSIAFVILALIICE
TIMVEPRHETQMAHSQSTYTKRREKLRSQSGSDDARNYQSVTYGKFQIMRLMDLLAPV
KKLWLKPDSAGSLVPRHTVILLIVLDILFVCGTTSCMPALILFSTYEYKWHAVELGYF
ISILGIGRGVVLLVVSPTLLYTLKRIYQHLNHSIDKIDIFCIQFSMIVITLSLFVMIR
FGEKTPTSMIIFALLQALSAFCSPTLQSGIIKYTSKKHTGEMFGAMALVRSCVMLVIP
PILLKLYGSTVSVNPSLFMYIPFSTSIVAILLTFFLRIYKNPPLDGP"
rep_origin 113678..113749
/note="ARS1007; Autonomously Replicating Sequence"
/db_xref="SGD:S000028444"
gene complement(<113878..>115629)
/gene="JJJ2"
/locus_tag="YJL162C"
/db_xref="GeneID:853277"
mRNA complement(<113878..>115629)
/gene="JJJ2"
/locus_tag="YJL162C"
/product="Jjj2p"
/transcript_id="NM_001181595.1"
/db_xref="GeneID:853277"
CDS complement(113878..115629)
/gene="JJJ2"
/locus_tag="YJL162C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="hypothetical protein; contains a J-domain, which is
a region with homology to the E. coli DnaJ protein"
/codon_start=1
/product="Jjj2p"
/protein_id="NP_012373.2"
/db_xref="GeneID:853277"
/db_xref="SGD:S000003698"
/translation="MSQVIEPQLDRTTYYSILGLTSNATSSEVHKSYLKLARLLHPDK
TKSDKSEELFKAVVHAHSILTDEDQKLRYDRDLKIKGLHTYQPKKNCHIFKTKAKESQ
GASPTLGQSEAYHRQNKPYEQQPYGFGVGKKMTSSSKSKVPIFKSFNLKSYQRNHYYS
SKKERKHGSPDIDSLFHETNGASKVRMTDAGKMDTNSQFQEIWEILGKNAYTHKSYSE
DPNSCLGSALSDHEEEEEAGKQQQQQQQQQQQQQHYGMTSKSSSPDEEKKNNKEPKRE
SRVSPEENGEEETGHKQFKLPKTSTFSSGSHDSNLQSPFYNHEYRHYARSKFECKNQF
RKSVSPIKEIPATTSANEGWNILRDIIEKLNISNVDDRNKDLLFRRDEIGDKNHSDSI
DIENLSIKEPKGMKRRKKDDISLEELFQSLPREKDYFMMDAINDSLESINLFKKPKTT
QSHEQGGTFAQAESNRAKFKPLLEQCGITPEILDLEIPEIPEFDAVADLETLKLNVQL
FNNQCNKLKETIHQVSLQRLRADTQFSDMLTQKQSIMVWKTYLEFDKSLMDKLNILQE
RQMQVIKIFSERCDGKV"
gene complement(115939..116010)
/locus_tag="YNCJ0002C"
/db_xref="GeneID:853278"
tRNA complement(115939..116010)
/locus_tag="YNCJ0002C"
/product="tRNA-Glu"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE
analysis; thiolation of uridine at wobble position (34)
requires Ncs6p; target of K. lactis zymocin"
/db_xref="GeneID:853278"
/db_xref="SGD:S000006557"
gene <117245..>117787
/gene="FMP33"
/locus_tag="YJL161W"
/db_xref="GeneID:853279"
mRNA <117245..>117787
/gene="FMP33"
/locus_tag="YJL161W"
/product="Fmp33p"
/transcript_id="NM_001181594.1"
/db_xref="GeneID:853279"
CDS 117245..117787
/gene="FMP33"
/locus_tag="YJL161W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278|PMID:14562095]"
/note="hypothetical protein; the authentic, non-tagged
protein is detected in highly purified mitochondria in
high-throughput studies"
/codon_start=1
/product="Fmp33p"
/protein_id="NP_012374.1"
/db_xref="GeneID:853279"
/db_xref="SGD:S000003697"
/translation="MLYTRLLRHNSQFTKFSGTSPNLGSKPLFSKGNLYTSLLVTTLY
GTGLACLYLESNSLNKSKEQEDPHAIAEDDIVNIVHDAPNRIFKPALDTYQEKELDLQ
KSDLHKVLHSLTYSDVSQFSIVWGFLIQLSSLIGNSTLGKKSILYKGSVVSVLGFPPL
IYMALKLRMKQLEKAGVRFE"
gene complement(<117962..>118825)
/gene="PIR5"
/locus_tag="YJL160C"
/db_xref="GeneID:853280"
mRNA complement(<117962..>118825)
/gene="PIR5"
/locus_tag="YJL160C"
/product="beta-1,3-glucan linked protein"
/transcript_id="NM_001181593.1"
/db_xref="GeneID:853280"
CDS complement(117962..118825)
/gene="PIR5"
/locus_tag="YJL160C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/note="hypothetical protein; member of the PIR (Proteins
with Internal Repeats) family of cell wall proteins;
SWAT-GFP fusion protein localizes to the endoplasmic
reticulum and vacuole, and mCherry fusion localizes to the
vacuole; non-essential gene that is required for
sporulation; mRNA is weakly cell cycle regulated, peaking
in mitosis; YJL160C has a paralog, PIR1, that arose from
the whole genome duplication"
/codon_start=1
/product="beta-1,3-glucan linked protein"
/protein_id="NP_012375.2"
/db_xref="GeneID:853280"
/db_xref="SGD:S000003696"
/translation="MHYKKAFLASLLSSIALTAYAPPEPWATLTPSSKMDGGTTEYRT
SFGLAVIPFTVTESKVKRNVISQINDGQVQVTTQKLPHPVSQIGDGQIQVTTQKVPPV
VSHIVSQIGDGQLQITTAKNVVTKSTIAVPSKTVTATATSTATAVSQIHDGQVQVTIS
SASSSSVLSKSKLEPTKKPNNEKVIKVQACKSSGTLAITLQGGVLIDSSGRIGSIVAN
RQFQFDGPPPQAGAIYAGGWSITKHGTLAIGDNDVFYQCLSGTFYNLYDQSIGGQCNP
VHLQTVGLVDC"
gene <120449..>121690
/gene="HSP150"
/locus_tag="YJL159W"
/gene_synonym="CCW7; ORE1; PIR2"
/db_xref="GeneID:853281"
mRNA <120449..>121690
/gene="HSP150"
/locus_tag="YJL159W"
/gene_synonym="CCW7; ORE1; PIR2"
/product="heat shock protein HSP150"
/transcript_id="NM_001181592.1"
/db_xref="GeneID:853281"
CDS 120449..121690
/gene="HSP150"
/locus_tag="YJL159W"
/gene_synonym="CCW7; ORE1; PIR2"
/experiment="EXISTENCE:direct assay:GO:0005199 structural
constituent of cell wall [PMID:10209754]"
/experiment="EXISTENCE:direct assay:GO:0005576
extracellular region [PMID:19129178]"
/experiment="EXISTENCE:direct assay:GO:0005621 cellular
bud scar [PMID:16278454]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:10209754|PMID:9192695|PMID:15781460]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:21446055]"
/experiment="EXISTENCE:mutant phenotype:GO:0016887 ATP
hydrolysis activity [PMID:21446055]"
/experiment="EXISTENCE:mutant phenotype:GO:0031505
fungal-type cell wall organization [PMID:10407261]"
/note="O-mannosylated heat shock protein; secreted and
covalently attached to the cell wall via beta-1,3-glucan
and disulfide bridges; required for cell wall stability;
induced by heat shock, oxidative stress, and nitrogen
limitation; HSP150 has a paralog, PIR3, that arose from
the whole genome duplication"
/codon_start=1
/product="heat shock protein HSP150"
/protein_id="NP_012376.3"
/db_xref="GeneID:853281"
/db_xref="SGD:S000003695"
/translation="MQYKKTLVASALAATTLAAYAPSEPWSTLTPTATYSGGVTDYAS
TFGIAVQPISTTSSASSAATTASSKAKRAASQIGDGQVQAATTTASVSTKSTAAAVSQ
IGDGQIQATTKTTAAAVSQIGDGQIQATTKTTSAKTTAAAVSQISDGQIQATTTTLAP
KSTAAAVSQIGDGQVQATTTTLAPKSTAAAVSQIGDGQVQATTKTTAAAVSQIGDGQV
QATTKTTAAAVSQIGDGQVQATTKTTAAAVSQIGDGQVQATTKTTAAAVSQITDGQVQ
ATTKTTQAASQVSDGQVQATTATSASAAATSTDPVDAVSCKTSGTLEMNLKGGILTDG
KGRIGSIVANRQFQFDGPPPQAGAIYAAGWSITPDGNLAIGDNDVFYQCLSGTFYNLY
DEHIGSQCTPVHLEAIDLIDC"
gene complement(<122265..>122948)
/gene="CIS3"
/locus_tag="YJL158C"
/gene_synonym="CCW11; CCW5; PIR4; SCW8"
/db_xref="GeneID:853282"
mRNA complement(<122265..>122948)
/gene="CIS3"
/locus_tag="YJL158C"
/gene_synonym="CCW11; CCW5; PIR4; SCW8"
/product="Cis3p"
/transcript_id="NM_001181591.1"
/db_xref="GeneID:853282"
CDS complement(122265..122948)
/gene="CIS3"
/locus_tag="YJL158C"
/gene_synonym="CCW11; CCW5; PIR4; SCW8"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005576
extracellular region [PMID:19129178|PMID:12702350]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:10438739]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:15781460|PMID:10438739]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0031505
fungal-type cell wall organization [PMID:10407261]"
/note="Mannose-containing glycoprotein constituent of the
cell wall; member of the PIR (proteins with internal
repeats) family"
/codon_start=1
/product="Cis3p"
/protein_id="NP_012377.1"
/db_xref="GeneID:853282"
/db_xref="SGD:S000003694"
/translation="MQFKNVALAASVAALSATASAEGYTPGEPWSTLTPTGSISCGAA
EYTTTFGIAVQAITSSKAKRDVISQIGDGQVQATSAATAQATDSQAQATTTATPTSSE
KISSSASKTSTNATSSSCATPSLKDSSCKNSGTLELTLKDGVLTDAKGRIGSIVANRQ
FQFDGPPPQAGAIYAAGWSITEDGYLALGDSDVFYQCLSGNFYNLYDQNVAEQCSAIH
LEAVSLVDC"
gene complement(<123836..>126328)
/gene="FAR1"
/locus_tag="YJL157C"
/db_xref="GeneID:853283"
mRNA complement(<123836..>126328)
/gene="FAR1"
/locus_tag="YJL157C"
/product="cyclin-dependent protein serine/threonine kinase
inhibiting protein FAR1"
/transcript_id="NM_001181590.1"
/db_xref="GeneID:853283"
CDS complement(123836..126328)
/gene="FAR1"
/locus_tag="YJL157C"
/experiment="EXISTENCE:direct assay:GO:0004861
cyclin-dependent protein serine/threonine kinase inhibitor
activity [PMID:8066461]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9822386|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922|PMID:9822386]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:14988725]"
/experiment="EXISTENCE:direct assay:GO:0120171
Cdc24p-Far1p-Gbetagamma complex [PMID:10087263]"
/experiment="EXISTENCE:mutant phenotype:GO:0000749
response to pheromone triggering conjugation with cellular
fusion [PMID:7490290|PMID:10087263]"
/experiment="EXISTENCE:mutant phenotype:GO:0000751 mitotic
cell cycle G1 arrest in response to pheromone
[PMID:2147873]"
/experiment="EXISTENCE:mutant phenotype:GO:0043577
chemotropism [PMID:7490290|PMID:10087263]"
/experiment="EXISTENCE:mutant phenotype:GO:0051457
maintenance of protein location in nucleus
[PMID:10655592]"
/experiment="EXISTENCE:physical interaction:GO:0120171
Cdc24p-Far1p-Gbetagamma complex [PMID:10087263]"
/note="CDK inhibitor and nuclear anchor; during the cell
cycle Far1p sequesters the GEF Cdc24p in the nucleus;
phosphorylation by Cdc28p-Cln results in SCFCdc4
complex-mediated ubiquitin-dependent degradation,
releasing Cdc24p for export and activation of GTPase
Cdc42p; in response to pheromone, phosphorylation of Far1p
by MAPK Fus3p results in association with, and inhibition
of Cdc28p-Cln, as well as Msn5p mediated nuclear export of
Far1p-Cdc24p, targeting Cdc24p to polarity sites"
/codon_start=1
/product="cyclin-dependent protein serine/threonine kinase
inhibiting protein FAR1"
/protein_id="NP_012378.1"
/db_xref="GeneID:853283"
/db_xref="SGD:S000003693"
/translation="MKTPTRVSFEKKIHTPPSGDRDAERSPPKKFLRGLSGKVFRKTP
EFKKQQMPTFGYIEESQFTPNLGLMMSKRGNIPKPLNLSKPISPPPSLKKTAGSVASG
FSKTGQLSALQSPVNITSSNKYNIKATNLTTSLLRESISDSTTMCDTLSDINLTVMDE
DYRIDGDSYYEEDSPTFMISLERNIKKCNSQFSPKRYIGEKCLICEESISSTFTGEKV
VESTCSHTSHYNCYLMLFETLYFQGKFPECKICGEVSKPKDKDIVPEMVSKLLTGAGA
HDDGPSSNMQQQWIDLKTARSFTGEFPQFTPQEQLIRTADISCDGFRTPRLSNSNQFE
AVSYLDSPFLNSPFVNKMATTDPFDLSDDEKLDCDDEIDESAAEVWFSKTGGEHVMVS
VKFQEMRTSDDLGVLQDVNHVDHEELEEREKEWKKKIDQYIETNVDKDSEFGSLILFD
KLMYSDDGEQWVDNNLVILFSKFLVLFDFEEMKILGKIPRDQFYQVIKFNEDVLLCSL
KSTNIPEIYLRFNENCEKWLLPKWKYCLENSSLETLPLSEIVSTVKELSHVNIIGALG
APPDVISAQSHDSRLPWKRLHSDTPLKLIVCLNLSHADGELYRKRVLKSVHQILDGLN
TDDLLGIVVVGRDGSGVVGPFGTFIGMINKNWDGWTTFLDNLEVVNPNVFRDEKQQYK
VTLQTCERLASTSAYVDTDDHIATGYAKQILVLNGSDVVDIEHDQKLKKAFDQLSYHW
RYEISQRRMTPLNASIKQFLEELHTKRYLDVTLRLPQATFEQVYLGDMAAGEQKTRLI
MDEHPHSSLIEIEYFDLVKQQRIHQTLEVPNL"
gene complement(<126853..>128952)
/gene="SSY5"
/locus_tag="YJL156C"
/gene_synonym="RAA3"
/db_xref="GeneID:853285"
mRNA complement(<126853..>128952)
/gene="SSY5"
/locus_tag="YJL156C"
/gene_synonym="RAA3"
/product="Ssy5p"
/transcript_id="NM_001181589.1"
/db_xref="GeneID:853285"
CDS complement(126853..128952)
/gene="SSY5"
/locus_tag="YJL156C"
/gene_synonym="RAA3"
/experiment="EXISTENCE:direct assay:GO:0004252 serine-type
endopeptidase activity [PMID:29661936|PMID:16778074]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:11154269]"
/experiment="EXISTENCE:direct assay:GO:0016485 protein
processing [PMID:16778074]"
/experiment="EXISTENCE:genetic interaction:GO:0043200
response to amino acid [PMID:11154269]"
/experiment="EXISTENCE:mutant phenotype:GO:0004252
serine-type endopeptidase activity [PMID:16524914]"
/experiment="EXISTENCE:mutant phenotype:GO:0016485 protein
processing [PMID:16524914]"
/experiment="EXISTENCE:mutant phenotype:GO:0016540 protein
autoprocessing [PMID:29661936]"
/experiment="EXISTENCE:mutant phenotype:GO:0043200
response to amino acid [PMID:11154269]"
/note="Serine protease of SPS plasma membrane amino acid
sensor system; contains an inhibitory domain that
dissociates in response to extracellular amino acids,
freeing a catalytic domain to activate transcription
factor Stp1p; other members are Ssy1p and Ptr3p"
/codon_start=1
/product="Ssy5p"
/protein_id="NP_012379.2"
/db_xref="GeneID:853285"
/db_xref="SGD:S000003692"
/translation="MVRFFGLNKKKNEEKENTDLPADNEQNAAETSSSNVSGNEERID
PNSHDTNPENANNDDASTTFGSSIQSSSIFSRGRMTYGTGASSSMATSEMRSHSSGHS
GSKNSKNLQGFKDVGKPLRAVSFLSPVKEEESQDTQNTLDVSSSTSSTLATSENAREN
SFTSRRSITLEYIHKSLSELEENLVDIMDDIHQDVISISKAVIEAIEYFKEFLPTTRD
RIPYRISLEKSSSLRKINKIVLHFLDNLLVSDAFSNSRSILLRRFYFFLKKLNLITDD
DLISESGVLPCLSVFCIGSHCNLPSMDKLGMILDELTKMDSSIISDQEGAFIAPILRG
ITPKSSILTIMFGLPNLQHEHYEMIKVLYSLFPDVHMYCVKDYIKKAASAVGSIPSHT
AATIDTIAPTKFQFSPPYAVSENPLELPISMSLSTETSAKITGTLGGYLFPQTGSDKK
FSQFASCSFAITCAHVVLSEKQDYPNVMVPSNVLQTSYKKVLTKESDRYPDGSVEKTA
FLEEVQRIDQNLNWQKSNKFGQVVWGERAIVDHRLSDFAIIKVNSSFKCQNTLGNGLK
SFPDPTLRFQNLHVKRKIFKMKPGMKVFKIGASTGYTSGELNSTKLVYWADGKLQSSE
FVVASPTPLFASAGDSGAWILTKLEDRLGLGLVGMLHSYDGEQRQFGLFTPIGDILER
LHAVTKIQWDIDPQLDG"
gene complement(<129285..>130643)
/gene="FBP26"
/locus_tag="YJL155C"
/db_xref="GeneID:853286"
mRNA complement(<129285..>130643)
/gene="FBP26"
/locus_tag="YJL155C"
/product="fructose-2,6-bisphosphatase"
/transcript_id="NM_001181588.1"
/db_xref="GeneID:853286"
CDS complement(129285..130643)
/gene="FBP26"
/locus_tag="YJL155C"
/EC_number="3.1.3.46"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0004331
fructose-2,6-bisphosphate 2-phosphatase activity
[PMID:1322693]"
/experiment="EXISTENCE:mutant phenotype:GO:0006006 glucose
metabolic process [PMID:1322693]"
/note="Fructose-2,6-bisphosphatase, required for glucose
metabolism; protein abundance increases in response to DNA
replication stress"
/codon_start=1
/product="fructose-2,6-bisphosphatase"
/protein_id="NP_012380.1"
/db_xref="GeneID:853286"
/db_xref="SGD:S000003691"
/translation="MGYSTISNDNDIKVCVIMVGLPARGKSFISQKIIRYLSWLSIKA
KCFNVGNYRRDVSGNVPMDAEFFNFENTDNFKLRELAAQNAIKDIVNFFTKEDGSVAV
FDATNSTRKRRKWLKDICEKNNIQPMFLESWSNDHELIINNAKDIGSTSPDYENSEPH
VAEADFLERIRQYERFYEPLDPQKDKDMTFIKLVNIIEEVVINKIRTYLESRIVFYVM
NIRPKPKYIWLSRHGESIYNVEKKIGGDSSLSERGFQYAKKLEQLVKESAGEINLTVW
TSTLKRTQQTANYLPYKKLQWKALDELDAGVCDGMTYEEIEKEYPEDFKARDNDKYEY
RYRGGESYRDVVIRLEPVIMELERQENVLIITHQAVLRCIYAYFMNVPQEESPWMSIP
LHTLIKLEPRAYGTKVTKIKANIPAVSTYKEKGTSQVGELSQSSTKLHQLLNDSPLED
KF"
gene complement(<131101..>133935)
/gene="VPS35"
/locus_tag="YJL154C"
/gene_synonym="ENV1; GRD9; VPT7"
/db_xref="GeneID:853287"
mRNA complement(<131101..>133935)
/gene="VPS35"
/locus_tag="YJL154C"
/gene_synonym="ENV1; GRD9; VPT7"
/product="retromer subunit VPS35"
/transcript_id="NM_001181587.1"
/db_xref="GeneID:853287"
CDS complement(131101..133935)
/gene="VPS35"
/locus_tag="YJL154C"
/gene_synonym="ENV1; GRD9; VPT7"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:22593205]"
/experiment="EXISTENCE:direct assay:GO:0170071 CROP
complex [PMID:35466426]"
/experiment="EXISTENCE:mutant phenotype:GO:0030904
retromer complex [PMID:9700157]"
/experiment="EXISTENCE:mutant phenotype:GO:0045053 protein
retention in Golgi apparatus [PMID:8649377]"
/experiment="EXISTENCE:mutant phenotype:GO:0140312 cargo
adaptor activity [PMID:9700157]"
/experiment="EXISTENCE:physical interaction:GO:0005768
endosome [PMID:9700157]"
/experiment="EXISTENCE:physical interaction:GO:0030906
retromer, cargo-selective complex [PMID:9700157]"
/experiment="EXISTENCE:physical interaction:GO:0042147
retrograde transport, endosome to Golgi [PMID:9700157]"
/experiment="EXISTENCE:physical interaction:GO:0140312
cargo adaptor activity [PMID:9700157]"
/note="Endosomal subunit of membrane-associated retromer
complex; required for retrograde transport; receptor that
recognizes retrieval signals on cargo proteins, forms
subcomplex with Vps26p and Vps29p that selects cargo
proteins for retrieval; interacts with Ypt7p;
overexpression of wild-type human VPS35 or
Parkinson's-associated vps35-D686N or vps35-P299S variants
complements Ni2+ resistance and Cd2+ sensitivity of yeast
vps35 null mutant"
/codon_start=1
/product="retromer subunit VPS35"
/protein_id="NP_012381.1"
/db_xref="GeneID:853287"
/db_xref="SGD:S000003690"
/translation="MAYADSPENAIAVIKQRTALMNRCLSQHKLMESLQHTSIMLTEL
RNPNLSPKKYYELYVIIFDSLTNLSTYLIENHPQNHHLADLYELVQYTGNVVPRLYLM
ITVGTSYLTFNEAPKKEILKDMIEMCRGVQNPIRGLFLRYYLSQRTKELLPEDDPSFN
SQFIMNNFIEMNKLWVRLQHQGPLRERETRTRERKELQILVGSQLVRLSQIIDDNFQM
YKQDILPTILEQVIQCRDLVSQEYLLDVICQVFADEFHLKTLDTLLQTTLHLNPDVSI
NKIVLTLVDRLNDYVTRQLEDDPNATSTNAYLDMDVFGTFWDYLTVLNHERPDLSLQQ
FIPLVESVIVLSLKWYPNNFDNLNKLFELVLQKTKDYGQKNISLESEHLFLVLLSFQN
SKLQLTSSTTAPPNSPVTSKKHFIFQLISQCQAYKNILALQSISLQKKVVNEIIDILM
DREVEEMADNDSESKLHPPGHSAYLVIEDKLQVQRLLSICEPLIISRSGPPANVASSD
TNVDEVFFNRHDEEESWILDPIQEKLAHLIHWIMNTTSRKQTMKNKIQFSLEAQLEIL
LLIKSSFIKGGINVKYTFPAIITNFWKLMRKCRMIQEYLLKKRPDNKTLLSHYSNLLK
QMFKFVSRCINDIFNSCNNSCTDLILKLNLQCAILADQLQLNEISYDFFSQAFTIFEE
SLSDSKTQLQALIYIAQSLQKTRSLYKEAYYDSLIVRCTLHGSKLLKKQDQCRAVYLC
SHLWWATEISNIGEEEGITDNFYRDGKRVLECLQRSLRVADSIMDNEQSCELMVEILN
RCLYYFIHGDESETHISIKYINGLIELIKTNLKSLKLEDNSASMITNSISDLHITGEN
NVKASSNADDGSVITDKESNVAIGSDGTYIQLNTLNGSSTLIRGVVATASGSKLLHQL
KYIPIHHFRRTCEYIESQREVDDRFKVIYV"
gene complement(<134332..>135933)
/gene="INO1"
/locus_tag="YJL153C"
/gene_synonym="APR1"
/db_xref="GeneID:853288"
mRNA complement(<134332..>135933)
/gene="INO1"
/locus_tag="YJL153C"
/gene_synonym="APR1"
/product="inositol-3-phosphate synthase INO1"
/transcript_id="NM_001181586.1"
/db_xref="GeneID:853288"
CDS complement(134332..135933)
/gene="INO1"
/locus_tag="YJL153C"
/gene_synonym="APR1"
/EC_number="5.5.1.4"
/experiment="EXISTENCE:direct assay:GO:0004512
inositol-3-phosphate synthase activity [PMID:7016881]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0006021
inositol biosynthetic process [PMID:7016881]"
/note="Inositol-3-phosphate synthase; involved in
synthesis of inositol phosphates and inositol-containing
phospholipids; transcription is coregulated with other
phospholipid biosynthetic genes by Ino2p and Ino4p, which
bind the UASINO DNA element"
/codon_start=1
/product="inositol-3-phosphate synthase INO1"
/protein_id="NP_012382.2"
/db_xref="GeneID:853288"
/db_xref="SGD:S000003689"
/translation="MTEDNIAPITSVKVVTDKCTYKDNELLTKYSYENAVVTKTASGR
FDVTPTVQDYVFKLDLKKPEKLGIMLIGLGGNNGSTLVASVLANKHNVEFQTKEGVKQ
PNYFGSMTQCSTLKLGIDAEGNDVYAPFNSLLPMVSPNDFVVSGWDINNADLYEAMQR
SQVLEYDLQQRLKAKMSLVKPLPSIYYPDFIAANQDERANNCINLDEKGNVTTRGKWT
HLQRIRRDIQNFKEENALDKVIVLWTANTERYVEVSPGVNDTMENLLQSIKNDHEEIA
PSTIFAAASILEGVPYINGSPQNTFVPGLVQLAEHEGTFIAGDDLKSGQTKLKSVLAQ
FLVDAGIKPVSIASYNHLGNNDGYNLSAPKQFRSKEISKSSVIDDIIASNDILYNDKL
GKKVDHCIVIKYMKPVGDSKVAMDEYYSELMLGGHNRISIHNVCEDSLLATPLIIDLL
VMTEFCTRVSYKKVDPVKEDAGKFENFYPVLTFLSYWLKAPLTRPGFHPVNGLNKQRT
ALENFLRLLIGLPSQNELRFEERLL"
gene complement(<136372..>136773)
/gene="SNA3"
/locus_tag="YJL151C"
/db_xref="GeneID:853290"
mRNA complement(<136372..>136773)
/gene="SNA3"
/locus_tag="YJL151C"
/product="Sna3p"
/transcript_id="NM_001181584.1"
/db_xref="GeneID:853290"
CDS complement(136372..136773)
/gene="SNA3"
/locus_tag="YJL151C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:22761830|PMID:23708375]"
/experiment="EXISTENCE:direct assay:GO:0000328 fungal-type
vacuole lumen [PMID:12499386|PMID:17182850]"
/experiment="EXISTENCE:direct assay:GO:0031625 ubiquitin
protein ligase binding [PMID:17182849|PMID:17332499]"
/experiment="EXISTENCE:mutant phenotype:GO:0030163 protein
catabolic process [PMID:19944104]"
/experiment="EXISTENCE:mutant phenotype:GO:0031625
ubiquitin protein ligase binding
[PMID:17182849|PMID:17332499]"
/experiment="EXISTENCE:mutant phenotype:GO:0032509
endosome transport via multivesicular body sorting pathway
[PMID:22212814]"
/experiment="EXISTENCE:physical interaction:GO:0031625
ubiquitin protein ligase binding
[PMID:17182849|PMID:17332499]"
/note="Protein involved in efficient MVB sorting of
proteins to the vacuole; may function as an RSP5 adapter
protein for MVB cargos; integral membrane protein
localized to vacuolar intralumenal vesicles"
/codon_start=1
/product="Sna3p"
/protein_id="NP_012384.1"
/db_xref="GeneID:853290"
/db_xref="SGD:S000003687"
/translation="MDRDHINDHDHRMSYSINKDDLLLMVLAVFIPPVAVWKRKGMFN
RDTLLNLLLFLLLFFPAIIHACYVVYETSSERSYDLSRRHATAPAVDRDLEAHPAEES
QAQPPAYDEDDEAGADVPLMDNKQQLSSGRT"
gene <137379..>139370
/gene="DAS1"
/locus_tag="YJL149W"
/gene_synonym="DUH1"
/db_xref="GeneID:853292"
mRNA <137379..>139370
/gene="DAS1"
/locus_tag="YJL149W"
/gene_synonym="DUH1"
/product="SCF ubiquitin ligase complex subunit DAS1"
/transcript_id="NM_001181582.1"
/db_xref="GeneID:853292"
CDS 137379..139370
/gene="DAS1"
/locus_tag="YJL149W"
/gene_synonym="DUH1"
/experiment="EXISTENCE:direct assay:GO:0019005 SCF
ubiquitin ligase complex [PMID:11283612|PMID:14747994]"
/experiment="EXISTENCE:physical interaction:GO:0019005 SCF
ubiquitin ligase complex [PMID:14747994]"
/note="Putative SCF ubiquitin ligase F-box protein;
interacts physically with both Cdc53p and Skp1 and
genetically with CDC34; similar to putative F-box protein
YDR131C"
/codon_start=1
/product="SCF ubiquitin ligase complex subunit DAS1"
/protein_id="NP_012386.1"
/db_xref="GeneID:853292"
/db_xref="SGD:S000003685"
/translation="MPFQDYFQKKKAAFINRNNKSNADASALRDINDININFAAKSKN
YVFPLTKLPDELMQEVFSHLPQPDRLQLCLVNKRLNKIATKLLYRRIYLNDSNVVKSD
FMHLAINWTLLNLPSSLKEEESRDIANCKLKKLIETLQNNIHITEVIQWIRINWDLDS
TLQRSILSILCNQGKSLQRLENVTDPACNDIISNGHFSRSNVSSFDMAPPNSLPEMVV
PENYIPNLTKYLSQRISSRLSHMTLFIDPLKLFNYLYPLDIKLQIIDLKLHWRREFYN
NDYFVKKIRPGNPLTKLSEVFDKRTLKILTIISWNDTLLKRETEMLKDFKEFENLEDL
SLISIKQDVHILVDLFSSLTNLKRLKMDFLEEYVPEPTNPHIFLSILLACSKLQFIDL
RYDGLIPQIINIQENKFQLNQQCNCTNCQIVFSDILKGKIFMFPEDYYIHDLQDIAAK
DIFKMMKYLSLLPYSKACDAYPSVRTQPMNLTNFVTKMNRNLLEYRNSKSQLVPKIVN
NPHQHSTVTSTSTAHMSEPEMIIIDDDDDDDEINAAIPPSSDDTAATISTDLELPHES
LTKRDIIMCYHALIHHFKSIYVTFLKSFPHLRFLMLNDIPTIVMEENNERIFEPVFYH
YDYKSNLYGWSKESNKNLENDSNNNNNNSDTIARIATVM"
gene complement(139566..139691)
/gene="SNR128"
/locus_tag="YNCJ0003C"
/db_xref="GeneID:9164909"
ncRNA complement(139566..139691)
/ncRNA_class="snoRNA"
/gene="SNR128"
/locus_tag="YNCJ0003C"
/product="SNR128"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:2850487]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:31804585|PMID:22266655]"
/experiment="EXISTENCE:direct assay:GO:0031428 box C/D
methylation guide snoRNP complex [PMID:10094313]"
/experiment="EXISTENCE:genetic interaction:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:7557394]"
/experiment="EXISTENCE:genetic interaction:GO:0019843 rRNA
binding [PMID:7557394]"
/experiment="EXISTENCE:mutant phenotype:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:7557394|PMID:2406561]"
/experiment="EXISTENCE:mutant phenotype:GO:0019843 rRNA
binding [PMID:7557394]"
/experiment="EXISTENCE:physical interaction:GO:0031428 box
C/D methylation guide snoRNP complex
[PMID:10733567|PMID:11081632]"
/note="C/D box small nucleolar RNA (snoRNA); referred to
as U14; essential gene required for cleavage of 35S
primary rRNA transcript to release the precursor to the
18S rRNA; guides 2'-O-methylation of small subunit (SSU)
rRNA at position C414"
/transcript_id="NR_132193.1"
/db_xref="GeneID:9164909"
/db_xref="SGD:S000006508"
gene complement(139761..139950)
/gene="SNR190"
/locus_tag="YNCJ0004C"
/db_xref="GeneID:9164910"
ncRNA complement(139761..139950)
/ncRNA_class="snoRNA"
/gene="SNR190"
/locus_tag="YNCJ0004C"
/product="SNR190"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:2850487]"
/experiment="EXISTENCE:direct assay:GO:0031428 box C/D
methylation guide snoRNP complex [PMID:10094313]"
/experiment="EXISTENCE:mutant phenotype:GO:0000470
maturation of LSU-rRNA [PMID:34686656]"
/experiment="EXISTENCE:mutant phenotype:GO:0042273
ribosomal large subunit biogenesis [PMID:34686656]"
/experiment="EXISTENCE:mutant phenotype:GO:0140691 RNA
folding chaperone [PMID:34686656]"
/experiment="EXISTENCE:physical interaction:GO:0031428 box
C/D methylation guide snoRNP complex
[PMID:10733567|PMID:11081632]"
/note="C/D box small nucleolar RNA (snoRNA); predicted to
guide 2'-O-methylation of large subunit (LSU) rRNA at
position G2395; not shown to be active"
/transcript_id="NR_132194.1"
/db_xref="GeneID:9164910"
/db_xref="SGD:S000006509"
gene <140437..>141138
/gene="RPA34"
/locus_tag="YJL148W"
/gene_synonym="CST21"
/db_xref="GeneID:853293"
mRNA <140437..>141138
/gene="RPA34"
/locus_tag="YJL148W"
/gene_synonym="CST21"
/product="DNA-directed RNA polymerase I subunit RPA34"
/transcript_id="NM_001181581.1"
/db_xref="GeneID:853293"
CDS 140437..141138
/gene="RPA34"
/locus_tag="YJL148W"
/gene_synonym="CST21"
/experiment="EXISTENCE:direct assay:GO:0003899
DNA-directed RNA polymerase activity [PMID:9837969]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12206772]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:12206772]"
/experiment="EXISTENCE:direct assay:GO:0005736 RNA
polymerase I complex
[PMID:9837969|PMID:11486042|PMID:9121426]"
/experiment="EXISTENCE:direct assay:GO:0006362
transcription elongation by RNA polymerase I
[PMID:18160037]"
/experiment="EXISTENCE:genetic interaction:GO:0042790
nucleolar large rRNA transcription by RNA polymerase I
[PMID:9121426]"
/experiment="EXISTENCE:mutant phenotype:GO:0005736 RNA
polymerase I complex [PMID:9121426]"
/note="RNA polymerase I subunit A34.5; essential for
nucleolar assembly and for high polymerase loading rate;
nucleolar localization depends on Rpa49p"
/codon_start=1
/product="DNA-directed RNA polymerase I subunit RPA34"
/protein_id="NP_012387.1"
/db_xref="GeneID:853293"
/db_xref="SGD:S000003684"
/translation="MSKLSKDYVSDSDSDDEVISNEFSIPDGFKKCKHLKNFPLNGDN
KKKAKQQQVWLIKFPSNVDISKLKSLPVDFESSTTMTIDKHDYKIMDDTDIESSLTQD
NLSNMTLLVPSESKESLKIASTAKDNAPLQFDKVFSVSETAKIPAIDYSKVRVPRKDV
PKVEGLKLEHFATGYDAEDFHVAEEVKENKKEPKKRSHHDDEEESSEKKKKKKEKREK
REKKDKKDKKKKHRD"
gene complement(<141419..>142567)
/gene="SMT1"
/locus_tag="YJL147C"
/gene_synonym="MRX5"
/db_xref="GeneID:853294"
mRNA complement(<141419..>142567)
/gene="SMT1"
/locus_tag="YJL147C"
/gene_synonym="MRX5"
/product="Smt1p"
/transcript_id="NM_001181580.1"
/db_xref="GeneID:853294"
CDS complement(141419..142567)
/gene="SMT1"
/locus_tag="YJL147C"
/gene_synonym="MRX5"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095]"
/note="Translational repressor of the mitochondrial ATP6/8
mRNA; homozygous diploid deletion strain has a sporulation
defect characterized by elevated dityrosine in the soluble
fraction; expression induced by calcium shortage"
/codon_start=1
/product="Smt1p"
/protein_id="NP_012388.1"
/db_xref="GeneID:853294"
/db_xref="SGD:S000003683"
/translation="MRRTFSQLATRLLKSKDDELKSTLKYLTKGPVKLLGPLFESSEV
NEQGSLLNRSRTKENNLQNHHIENILRILNSNLPEVESKKQKVAVHYDVLFSHLNSIV
TQATDNKSSSSKELQGSSSEDLYDRLLLLQYVGKLTNVRQITEILLSKKFNKFDKVWE
HRALFDEYQRVVISILLYYRTHDVQIRKDYEPRWLSDYSDLPFPLRRLLWRCLTSNVS
EDNIRQNIIHYIKLLGANWRNNDLILIYQSLYEKSHILPDLTVLNHNKDDGFSFTQNQ
ILLVRILRAISKCVEEEPKLVKKWLIDIVKLSIQSKIMLESPKKPSTPIMDQYKFIRS
LDISIRSIHRTCQDKLIFEDLQVNLGSVLKMINEEEHELKTHLPLNLI"
gene <143292..>144701
/gene="IDS2"
/locus_tag="YJL146W"
/db_xref="GeneID:853295"
mRNA <143292..>144701
/gene="IDS2"
/locus_tag="YJL146W"
/product="Ids2p"
/transcript_id="NM_001181579.1"
/db_xref="GeneID:853295"
CDS 143292..144701
/gene="IDS2"
/locus_tag="YJL146W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0051087
protein-folding chaperone binding [PMID:30516470]"
/experiment="EXISTENCE:mutant phenotype:GO:0006457 protein
folding [PMID:30516470]"
/experiment="EXISTENCE:mutant phenotype:GO:0051321 meiotic
cell cycle [PMID:7565676]"
/note="Protein involved in modulation of Ime2p activity
during meiosis; appears to act indirectly to promote
Ime2p-mediated late meiotic functions; found in growing
cells and degraded during sporulation"
/codon_start=1
/product="Ids2p"
/protein_id="NP_012389.1"
/db_xref="GeneID:853295"
/db_xref="SGD:S000003682"
/translation="MDNQQESISEDITGDLAAAVRKSWSESQDNPLLLNFNNSPIGTP
TDRYSPEPATMMEGNAMNLSSLARGSTQQQQRLYGSSQTREKSDQQQQDYQLFKHHYS
LGQETRESVSDILNDLTLGSPEPSERASPIRQPSVDVPPLTTRRSSIQDVQWIRHLLN
PRSSFSGASSNEPTNSPGDFLNQSRAWITILHDSSAESLQAVIVLAESLKNVNSQYNL
WVLHSSEVNAFQLAQVGIKTLIIDEYINLFMNFGTGSGFSASSQSTETKGELNFKWCK
LFLFFSLIDRFELICYLSPTCLVLQNIDELLESTEVSDEIDNETCVLLSNKVNYINED
LVSVNQDQSSAENYDDDPQIIILKPNRAVAMCIKEYFTIYGNDFEGESKRSMFHQMND
LQIMKALFGDKWSYIDSVGYCAVPIASVPANRLNYKIIEFKILKPWERQNYIAAGQHR
ESIMNKWLDLWRDFLNQAN"
gene <145160..>146044
/gene="SFH5"
/locus_tag="YJL145W"
/db_xref="GeneID:853296"
mRNA <145160..>146044
/gene="SFH5"
/locus_tag="YJL145W"
/product="Sfh5p"
/transcript_id="NM_001181578.1"
/db_xref="GeneID:853296"
CDS 145160..146044
/gene="SFH5"
/locus_tag="YJL145W"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:12869188|PMID:19477927]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:19477927]"
/experiment="EXISTENCE:direct assay:GO:0008526
phosphatidylinositol transfer activity [PMID:10848624]"
/experiment="EXISTENCE:direct assay:GO:0015914
phospholipid transport [PMID:10848624]"
/experiment="EXISTENCE:direct assay:GO:0020037 heme
binding [PMID:32780017]"
/experiment="EXISTENCE:direct assay:GO:0032541 cortical
endoplasmic reticulum [PMID:16262726]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762|PMID:12869188]"
/experiment="EXISTENCE:genetic interaction:GO:0017157
regulation of exocytosis [PMID:19477927]"
/experiment="EXISTENCE:genetic interaction:GO:0043001
Golgi to plasma membrane protein transport
[PMID:10848624]"
/experiment="EXISTENCE:genetic interaction:GO:0046488
phosphatidylinositol metabolic process [PMID:16262726]"
/experiment="EXISTENCE:genetic interaction:GO:2000114
regulation of establishment of cell polarity
[PMID:19477927]"
/note="Unusual phosphatidylinositol transfer protein
(PITP) that binds heme; similar to Sec14p; founding member
of conserved class of fungal hemoproteins; exhibits PI-
but not PC-transfer activity; localizes to peripheral
endoplasmic reticulum, cytosol, and microsomes; partially
relocalizes to plasma membrane upon DNA replication
stress"
/codon_start=1
/product="Sfh5p"
/protein_id="NP_012390.1"
/db_xref="GeneID:853296"
/db_xref="SGD:S000003681"
/translation="MKFDNDSEKQVFDKLKKAIPGIIKEKCAGYDELYGYKLNPEGLT
QEEVDKYYDEKIADRLTYKLCKAYQFEYSTIVQNLIDILNWRREFNPLSCAYKEVHNT
ELQNVGILTFDANGDANKKAVTWNLYGQLVKKKELFQNVDKFVRYRIGLMEKGLSLLD
FTSSDNNYMTQVHDYKGVSVWRMDSDIKNCSKTVIGIFQKYYPELLYAKYFVNVPTVF
GWVYDLIKKFVDETTRKKFVVLTDGSKLGQYLKDCPYEGYGGKDKKNNLTKQNVTNVH
PTEYGLYILQKQIIEDVE"
gene <146359..>146673
/gene="ROQ1"
/locus_tag="YJL144W"
/db_xref="GeneID:853297"
mRNA <146359..>146673
/gene="ROQ1"
/locus_tag="YJL144W"
/product="Roq1p"
/transcript_id="NM_001181577.1"
/db_xref="GeneID:853297"
CDS 146359..146673
/gene="ROQ1"
/locus_tag="YJL144W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0033554
cellular response to stress [PMID:29861160]"
/experiment="EXISTENCE:mutant phenotype:GO:0042631
cellular response to water deprivation [PMID:21420397]"
/experiment="EXISTENCE:mutant phenotype:GO:0055106
ubiquitin-protein transferase regulator activity
[PMID:29861160]"
/experiment="EXISTENCE:mutant phenotype:GO:0120174
stress-induced homeostatically regulated protein
degradation pathway [PMID:29861160]"
/experiment="EXISTENCE:physical interaction:GO:0033554
cellular response to stress [PMID:29861160]"
/experiment="EXISTENCE:physical interaction:GO:0055106
ubiquitin-protein transferase regulator activity
[PMID:29861160]"
/note="Ub-ligase substrate-specificity factor;
proteolytically-cleaved form acts as a pseudosubstrate,
binding and altering the substrate specificity of Ubr1p
towards misfolded and native ER membrane and cytosolic
proteins, as part of the stress-induced homeostatically
regulated protein degradation (SHRED) pathway; hydrophilin
essential during desiccation-rehydration; induced by
osmotic stress, starvation and during stationary phase;
protein abundance increases in response to DNA replication
stress"
/codon_start=1
/product="Roq1p"
/protein_id="NP_012391.1"
/db_xref="GeneID:853297"
/db_xref="SGD:S000003680"
/translation="MLRRETSTIYRTHKKSNSSILRSQRDQTRVDSLVEESPMGDFGI
NNQPTQPGVIYYFVELTNLGIQENTSSNNNNNNNHGDDENGSRYGHGSSLGGDVHSRR
CS"
gene <147101..>147577
/gene="TIM17"
/locus_tag="YJL143W"
/gene_synonym="MIM17; MPI2; SMS1"
/db_xref="GeneID:853298"
mRNA <147101..>147577
/gene="TIM17"
/locus_tag="YJL143W"
/gene_synonym="MIM17; MPI2; SMS1"
/product="protein transporter TIM17"
/transcript_id="NM_001181576.1"
/db_xref="GeneID:853298"
CDS 147101..147577
/gene="TIM17"
/locus_tag="YJL143W"
/gene_synonym="MIM17; MPI2; SMS1"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005744 TIM23
mitochondrial import inner membrane translocase complex
[PMID:15618217]"
/experiment="EXISTENCE:mutant phenotype:GO:0008320 protein
transmembrane transporter activity
[PMID:11344168|PMID:15618217]"
/experiment="EXISTENCE:mutant phenotype:GO:0030150 protein
import into mitochondrial matrix
[PMID:15618217|PMID:11344168|PMID:7600576|PMID:7919535]"
/experiment="EXISTENCE:physical interaction:GO:0005744
TIM23 mitochondrial import inner membrane translocase
complex [PMID:7600576]"
/note="Essential core component of the TIM23 protein
translocase complex; forms the protein translocation path
with Mgr2p, while Tim23p contributes to the architecture
of the import channel; may link the import motor to the
core translocase of the inner mitochondrial membrane
(TIM23 complex)"
/codon_start=1
/product="protein transporter TIM17"
/protein_id="NP_012392.1"
/db_xref="GeneID:853298"
/db_xref="SGD:S000003679"
/translation="MSADHSRDPCPIVILNDFGGAFAMGAIGGVVWHGIKGFRNSPLG
ERGSGAMSAIKARAPVLGGNFGVWGGLFSTFDCAVKAVRKREDPWNAIIAGFFTGGAL
AVRGGWRHTRNSSITCACLLGVIEGVGLMFQRYAAWQAKPMAPPLPEAPSSQPLQA"
gene complement(<147819..>148211)
/gene="IRC9"
/locus_tag="YJL142C"
/db_xref="GeneID:853299"
mRNA complement(<147819..>148211)
/gene="IRC9"
/locus_tag="YJL142C"
/product="Irc9p"
/transcript_id="NM_001395034.1"
/db_xref="GeneID:853299"
CDS complement(147819..148211)
/gene="IRC9"
/locus_tag="YJL142C"
/experiment="EXISTENCE:mutant phenotype:GO:0006312 mitotic
recombination [PMID:18085829]"
/experiment="EXISTENCE:mutant phenotype:GO:1990414
replication-born double-strand break repair via sister
chromatid exchange [PMID:23357952]"
/note="hypothetical protein; partially overlaps verified
gene YAK1/YJL141C but does not share all phenotypes; null
mutant displays increased levels of spontaneous Rad52p
foci, increased sporulation efficiency, and small defect
in vacuolar fragmentation"
/codon_start=1
/product="Irc9p"
/protein_id="NP_001381964.1"
/db_xref="GeneID:853299"
/db_xref="SGD:S000003678"
/translation="MTMQKASKVNMEVRTTLVTMQATTMSIILALPLVVLIASTLVLS
VKGRRIHLATSPIILLLLILFKKGQQARSINFTLSKNKSLFCLTFLNYPFHFITAWCH
NDILNKYEFCLFLIFLIRIVITINKVTL"
gene complement(<147967..>150390)
/gene="YAK1"
/locus_tag="YJL141C"
/db_xref="GeneID:853300"
mRNA complement(<147967..>150390)
/gene="YAK1"
/locus_tag="YJL141C"
/product="serine/threonine protein kinase YAK1"
/transcript_id="NM_001181574.1"
/db_xref="GeneID:853300"
CDS complement(147967..150390)
/gene="YAK1"
/locus_tag="YJL141C"
/EC_number="2.7.12.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0004674 protein
serine/threonine kinase activity [PMID:21255108]"
/experiment="EXISTENCE:direct assay:GO:0004713 protein
tyrosine kinase activity [PMID:21255108]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11358866|PMID:21255108]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:21255108|PMID:11358866]"
/experiment="EXISTENCE:genetic interaction:GO:0141156
cAMP/PKA signal transduction [PMID:8065298]"
/experiment="EXISTENCE:mutant phenotype:GO:0004674 protein
serine/threonine kinase activity [PMID:21255108]"
/experiment="EXISTENCE:mutant phenotype:GO:0004713 protein
tyrosine kinase activity [PMID:21255108]"
/note="Serine-threonine protein kinase; component of a
glucose-sensing system that inhibits growth in response to
glucose availability; upon nutrient deprivation Yak1p
phosphorylates Pop2p to regulate mRNA deadenylation, the
co-repressor Crf1p to inhibit transcription of ribosomal
genes, and the stress-responsive transcription factors
Hsf1p and Msn2p; nuclear localization negatively regulated
by the Ras/PKA signaling pathway in the presence of
glucose"
/codon_start=1
/product="serine/threonine protein kinase YAK1"
/protein_id="NP_012394.1"
/db_xref="GeneID:853300"
/db_xref="SGD:S000003677"
/translation="MNSSNNNDSSSSNSNMNNSLSPTLVTHSDASMGSGRASPDNSHM
GRGIWNPSYVNQGSQRSPQQQHQNHHQQQQQQQQQQQQNSQFCFVNPWNEEKVTNSQQ
NLVYPPQYDDLNSNESLDAYRRRKSSLVVPPARAPAPNPFQYDSYPAYTSSNTSLAGN
SSGQYPSGYQQQQQQVYQQGAIHPSQFGSRFVPSLYDRQDFQRRQSLAATNYSSNFSS
LNSNTNQGTNSIPVMSPYRRLSAYPPSTSPPLQPPFKQLRRDEVQGQKLSIPQMQLCN
SKNDLQPVLNATPKFRRASLNSKTISPLVSVTKSLITTYSLCSPEFTYQTSKNPKRVL
TKPSEGKCNNGFDNINSDYILYVNDVLGVEQNRKYLVLDILGQGTFGQVVKCQNLLTK
EILAVKVVKSRTEYLTQSITEAKILELLNQKIDPTNKHHFLRMYDSFVHKNHLCLVFE
LLSNNLYELLKQNKFHGLSIQLIRTFTTQILDSLCVLKESKLIHCDLKPENILLCAPD
KPELKIIDFGSSCEEARTVYTYIQSRFYRAPEIILGIPYSTSIDMWSLGCIVAELFLG
IPIFPGASEYNQLTRIIDTLGYPPSWMIDMGKNSGKFMKKLAPEESSSSTQKHRMKTI
EEFCREYNIVEKPSKQYFKWRKLPDIIRNYRYPKSIQNSQELIDQEMQNRECLIHFLG
GVLNLNPLERWTPQQAMLHPFITKQEFTGEWFPPGSSLPGPSEKHDDAKGQQSEYGSA
NDSSNNAGHNYVYNPSSATGGADSVDIGAISKRKENTSGDISNNFAVTHSVQEGPTSA
FNKLHIVEE"
gene <150961..>151626
/gene="RPB4"
/locus_tag="YJL140W"
/gene_synonym="CTF15"
/db_xref="GeneID:853301"
mRNA <150961..>151626
/gene="RPB4"
/locus_tag="YJL140W"
/gene_synonym="CTF15"
/product="DNA-directed RNA polymerase II subunit RPB4"
/transcript_id="NM_001181573.1"
/db_xref="GeneID:853301"
CDS 150961..151626
/gene="RPB4"
/locus_tag="YJL140W"
/gene_synonym="CTF15"
/experiment="EXISTENCE:direct assay:GO:0000932 P-body
[PMID:16357218]"
/experiment="EXISTENCE:direct assay:GO:0003697
single-stranded DNA binding [PMID:11087726]"
/experiment="EXISTENCE:direct assay:GO:0003727
single-stranded RNA binding [PMID:11087726]"
/experiment="EXISTENCE:direct assay:GO:0003968
RNA-directed RNA polymerase activity [PMID:18004386]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12857861]"
/experiment="EXISTENCE:direct assay:GO:0005665 RNA
polymerase II, core complex
[PMID:2186966|PMID:1331084|PMID:2183013]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:12857861]"
/experiment="EXISTENCE:direct assay:GO:0006367
transcription initiation at RNA polymerase II promoter
[PMID:11087726]"
/experiment="EXISTENCE:mutant phenotype:GO:0000288
nuclear-transcribed mRNA catabolic process,
deadenylation-dependent decay [PMID:16357218]"
/experiment="EXISTENCE:mutant phenotype:GO:0006366
transcription by RNA polymerase II [PMID:11577101]"
/experiment="EXISTENCE:mutant phenotype:GO:0045948
positive regulation of translational initiation
[PMID:21074047]"
/experiment="EXISTENCE:physical interaction:GO:0031369
translation initiation factor binding [PMID:21074047]"
/note="RNA polymerase II subunit B32; forms dissociable
heterodimer with Rpb7p; Rpb4/7 dissociates from RNAPII as
Ser2 CTD phosphorylation increases; Rpb4/7 regulates
cellular lifespan via mRNA decay process; involved in
recruitment of 3'-end processing factors to transcribing
RNAPII complex, export of mRNA to cytoplasm under stress
conditions; also involved in translation initiation"
/codon_start=1
/product="DNA-directed RNA polymerase II subunit RPB4"
/protein_id="NP_012395.1"
/db_xref="GeneID:853301"
/db_xref="SGD:S000003676"
/translation="MNVSTSTFQTRRRRLKKVEEEENAATLQLGQEFQLKQINHQGEE
EELIALNLSEARLVIKEALVERRRAFKRSQKKHKKKHLKHENANDETTAVEDEDDDLD
EDDVNADDDDFMHSETREKELESIDVLLEQTTGGNNKDLKNTMQYLTNFSRFRDQETV
GAVIQLLKSTGLHPFEVAQLGSLACDTADEAKTLIPSLNNKISDDELERILKELSNLE
TLY"
gene complement(<151713..>152999)
/gene="YUR1"
/locus_tag="YJL139C"
/db_xref="GeneID:853302"
mRNA complement(<151713..>152999)
/gene="YUR1"
/locus_tag="YJL139C"
/product="mannosyltransferase YUR1"
/transcript_id="NM_001181572.1"
/db_xref="GeneID:853302"
CDS complement(151713..152999)
/gene="YUR1"
/locus_tag="YJL139C"
/experiment="EXISTENCE:direct assay:GO:0000030
mannosyltransferase activity [PMID:8631921]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:8631921]"
/experiment="EXISTENCE:mutant phenotype:GO:0000032 cell
wall mannoprotein biosynthetic process [PMID:8631921]"
/experiment="EXISTENCE:mutant phenotype:GO:0006487 protein
N-linked glycosylation [PMID:8631921]"
/note="Mannosyltransferase involved in protein
N-glycosylation; member of the KTR1 family; located in the
Golgi apparatus; YUR1 has a paralog, KTR2, that arose from
the whole genome duplication"
/codon_start=1
/product="mannosyltransferase YUR1"
/protein_id="NP_012396.1"
/db_xref="GeneID:853302"
/db_xref="SGD:S000003675"
/translation="MAKGGSLYIVGIFLPIWTFMIYIFGKELFLIRKYQKIDSTYTAL
SQRVKEQYDTSRRRNYFPKVKLSRNSYDDYTLNYTRQNDSDSFHLRENATILMLVRNS
ELEGALDSMRSLEDRFNNKYHYDWTFLNDVPFDQDFIEATTSMASGKTQYALIPPEDW
NRPQWINDTLFEERLRVMEDEGVLYGGSKSYRNMCRFNSGFFFRQSILDNYDYYFRVE
PNVKYYCDFPYDPFRVMRLKGKKYGFVISLYEYEETIPTLWDAVEEYLVASEETILRK
EDSAYAFLTDSGLVGKHYPVVEANSDYNLCHFWSNFEIGDLNFFRSDEYKHFFETLDA
KGGFYYERWGDAPVHSIGVSLLLRPDEIIHFDELGYFHSPFGTCPASYAVRLDQRCRC
KSDDESVIDITPHSCLMRWWKNGSGKYFLKEEQDEI"
gene complement(<153504..>154691)
/gene="TIF2"
/locus_tag="YJL138C"
/db_xref="GeneID:853303"
mRNA complement(<153504..>154691)
/gene="TIF2"
/locus_tag="YJL138C"
/product="translation initiation factor eIF4A"
/transcript_id="NM_001181571.1"
/db_xref="GeneID:853303"
CDS complement(153504..154691)
/gene="TIF2"
/locus_tag="YJL138C"
/EC_number="3.6.4.13"
/experiment="EXISTENCE:direct assay:GO:0003724 RNA
helicase activity [PMID:1502180]"
/experiment="EXISTENCE:direct assay:GO:0003743 translation
initiation factor activity [PMID:1502180]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836]"
/experiment="EXISTENCE:direct assay:GO:0006413
translational initiation [PMID:1502180]"
/experiment="EXISTENCE:direct assay:GO:0008186
ATP-dependent activity, acting on RNA [PMID:24471916]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405]"
/experiment="EXISTENCE:direct assay:GO:1901195 positive
regulation of formation of translation preinitiation
complex [PMID:30281017|PMID:29192585]"
/experiment="EXISTENCE:mutant phenotype:GO:0003724 RNA
helicase activity [PMID:1502180]"
/experiment="EXISTENCE:mutant phenotype:GO:0003743
translation initiation factor activity [PMID:1502180]"
/experiment="EXISTENCE:mutant phenotype:GO:0006413
translational initiation [PMID:1502180]"
/experiment="EXISTENCE:mutant phenotype:GO:0016281
eukaryotic translation initiation factor 4F complex
[PMID:10409745|PMID:2648398]"
/note="Translation initiation factor eIF4A; DEAD-box RNA
helicase that couples ATPase activity to mRNA binding and
unwinding; ATP hydrolysis accelerates the recruitment of
mRNA during 48S preinitiation complex (PIC) assembly;
ATPase activity stimulated by either eIF4G1 or by the PIC
in an eIF3g and eIFGi subunit-dependent manner,
independent of eIF4E and eIF4G; subunit of the mRNA
cap-binding protein complex (eIF4F) with eIF4E and eIF4G;
protein abundance increases in response to DNA replication
stress"
/codon_start=1
/product="translation initiation factor eIF4A"
/protein_id="NP_012397.1"
/db_xref="GeneID:853303"
/db_xref="SGD:S000003674"
/translation="MSEGITDIEESQIQTNYDKVVYKFDDMELDENLLRGVFGYGFEE
PSAIQQRAIMPIIEGHDVLAQAQSGTGKTGTFSIAALQRIDTSVKAPQALMLAPTREL
ALQIQKVVMALAFHMDIKVHACIGGTSFVEDAEGLRDAQIVVGTPGRVFDNIQRRRFR
TDKIKMFILDEADEMLSSGFKEQIYQIFTLLPPTTQVVLLSATMPNDVLEVTTKFMRN
PVRILVKKDELTLEGIKQFYVNVEEEEYKYECLTDLYDSISVTQAVIFCNTRRKVEEL
TTKLRNDKFTVSAIYSDLPQQERDTIMKEFRSGSSRILISTDLLARGIDVQQVSLVIN
YDLPANKENYIHRIGRGGRFGRKGVAINFVTNEDVGAMRELEKFYSTQIEELPSDIAT
LLN"
gene complement(<154985..>156127)
/gene="GLG2"
/locus_tag="YJL137C"
/db_xref="GeneID:853304"
mRNA complement(<154985..>156127)
/gene="GLG2"
/locus_tag="YJL137C"
/product="glycogenin glucosyltransferase GLG2"
/transcript_id="NM_001181570.1"
/db_xref="GeneID:853304"
CDS complement(154985..156127)
/gene="GLG2"
/locus_tag="YJL137C"
/EC_number="2.4.1.186"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0005978
glycogen biosynthetic process [PMID:8524228]"
/experiment="EXISTENCE:genetic interaction:GO:0008466
glycogenin glucosyltransferase activity [PMID:8524228]"
/note="Glycogenin glucosyltransferase; self-glucosylating
initiator of glycogen synthesis, also glucosylates
n-dodecyl-beta-D-maltoside; similar to mammalian
glycogenin; GLG2 has a paralog, GLG1, that arose from the
whole genome duplication"
/codon_start=1
/product="glycogenin glucosyltransferase GLG2"
/protein_id="NP_012398.1"
/db_xref="GeneID:853304"
/db_xref="SGD:S000003673"
/translation="MAKKVAICTLLYSRDYLPGALTLAYQLQKLLKHAVVEDEITLCL
LIEKKLFGDEFKPQEIALIRSLFKEIIIIEPLKDQEKSIEKNKANLELLKRPELSHTL
LKARLWELVQFDQVLFLDADTLPLNKEFFEILRLYPEQTRFQIAAVPDIGWPDMFNTG
VLLLIPDLDMATSLQDFLIKTVSIDGADQGIFNQFFNPICNYSKEVLHKVSPLMEWIR
LPFTYNVTMPNYGYQSSPAMNFFQQHIRLIHFIGTFKPWSRNTTDYDDHYYQLWRSTQ
RELYSECHLSNYFTHLQLGNIETETNFYHEPPCLQDLLNHGKRENQKHVDLDITSVDR
NASQKSTAEKHDIEKPTSKPQSAFKFDWESTDYLDRVQRAFPKPDT"
gene <156169..>156252
/locus_tag="YJL136W-A"
/db_xref="GeneID:1466464"
mRNA <156169..>156252
/locus_tag="YJL136W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184637.1"
/db_xref="GeneID:1466464"
CDS 156169..156252
/locus_tag="YJL136W-A"
/note="hypothetical protein; identified by SAGE"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878101.1"
/db_xref="GeneID:1466464"
/db_xref="SGD:S000028806"
/translation="MTRCISKKMLLEVDALSLIYSPHLYMS"
gene complement(<156550..>157273)
/gene="RPS21B"
/locus_tag="YJL136C"
/db_xref="GeneID:853305"
mRNA complement(join(<156550..156789,157250..>157273))
/gene="RPS21B"
/locus_tag="YJL136C"
/product="40S ribosomal protein eS21 RPS21B"
/transcript_id="NM_001181569.1"
/db_xref="GeneID:853305"
CDS complement(join(156550..156789,157250..157273))
/gene="RPS21B"
/locus_tag="YJL136C"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:3533916]"
/experiment="EXISTENCE:direct assay:GO:0022627 cytosolic
small ribosomal subunit [PMID:3533916]"
/experiment="EXISTENCE:mutant phenotype:GO:0000447
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from
5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:14627813]"
/experiment="EXISTENCE:mutant phenotype:GO:0000461
endonucleolytic cleavage to generate mature 3'-end of
SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:14627813]"
/note="Protein component of the small (40S) ribosomal
subunit; homologous to mammalian ribosomal protein S21, no
bacterial homolog; RPS21B has a paralog, RPS21A, that
arose from the whole genome duplication"
/codon_start=1
/product="40S ribosomal protein eS21 RPS21B"
/protein_id="NP_012399.1"
/db_xref="GeneID:853305"
/db_xref="SGD:S000003672"
/translation="MENDKGQLVELYVPRKCSATNRIIKADDHASVQINVAKVDEEGR
AIPGEYITYALSGYVRSRGESDDSLNRLAQNDGLLKNVWSYSR"
gene <158188..>159417
/gene="LCB3"
/locus_tag="YJL134W"
/gene_synonym="LBP1; YSR2"
/db_xref="GeneID:853307"
mRNA <158188..>159417
/gene="LCB3"
/locus_tag="YJL134W"
/gene_synonym="LBP1; YSR2"
/product="sphinganine kinase LCB3"
/transcript_id="NM_001181567.1"
/db_xref="GeneID:853307"
CDS 158188..159417
/gene="LCB3"
/locus_tag="YJL134W"
/gene_synonym="LBP1; YSR2"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762|PMID:10477278]"
/experiment="EXISTENCE:direct assay:GO:0042392
sphingosine-1-phosphate phosphatase activity
[PMID:9353337]"
/experiment="EXISTENCE:mutant phenotype:GO:0019722
calcium-mediated signaling [PMID:11278643]"
/note="Long-chain base-1-phosphate phosphatase; specific
for dihydrosphingosine-1-phosphate, regulates ceramide and
long-chain base phosphates levels, involved in
incorporation of exogenous long chain bases in
sphingolipids; LCB3 has a paralog, YSR3, that arose from
the whole genome duplication"
/codon_start=1
/product="sphinganine kinase LCB3"
/protein_id="NP_012401.1"
/db_xref="GeneID:853307"
/db_xref="SGD:S000003670"
/translation="MVDGLNTSNIRKRARTLSNPNDFQEPNYLLDPGNHPSDHFRTRM
SKFRFNIREKLLVFTNNQSFTLSRWQKKYRSAFNDLYFTYTSLMGSHTFYVLCLPMPV
WFGYFETTKDMVYILGYSIYLSGFFKDYWCLPRPRAPPLHRITLSEYTTKEYGAPSSH
TANATGVSLLFLYNIWRMQESSVMVQLLLSCVVLFYYMTLVFGRIYCGMHGILDLVSG
GLIGIVCFIVRMYFKYRFPGLRIEEHWWFPLFSVGWGLLLLFKHVKPVDECPCFQDSV
AFMGVVSGIECCDWLGKVFGVTLVYNLEPNCGWRLTLARLLVGLPCVVIWKYVISKPM
IYTLLIKVFHLKDDRNVAARKRLEATHKEGASKYECPLYIGEPKIDILGRFIIYAGVP
FTVVMCSPVLFSLLNIA"
gene complement(<159623..>159847)
/gene="DPI8"
/locus_tag="YJL133C-A"
/db_xref="GeneID:1466465"
mRNA complement(<159623..>159847)
/gene="DPI8"
/locus_tag="YJL133C-A"
/product="Dpi8p"
/transcript_id="NM_001184636.1"
/db_xref="GeneID:1466465"
CDS complement(159623..159847)
/gene="DPI8"
/locus_tag="YJL133C-A"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/note="Putative mitochondrial hypothetical protein; the
authentic, non-tagged protein is detected in highly
purified mitochondria in high-throughput studies"
/codon_start=1
/product="Dpi8p"
/protein_id="NP_878102.1"
/db_xref="GeneID:1466465"
/db_xref="SGD:S000028805"
/translation="MIAQSTRLAAAVSSSAASAGVSRIAASAMASTIFKRSPGNSFNS
FKEYRENAKTYGPLSASLATRRHLAHAPKL"
gene <160619..>161563
/gene="MRS3"
/locus_tag="YJL133W"
/db_xref="GeneID:853308"
mRNA <160619..>161563
/gene="MRS3"
/locus_tag="YJL133W"
/product="Fe(2+) transporter"
/transcript_id="NM_001181566.1"
/db_xref="GeneID:853308"
CDS 160619..161563
/gene="MRS3"
/locus_tag="YJL133W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:1703236]"
/experiment="EXISTENCE:direct assay:GO:0048250 iron import
into the mitochondrion [PMID:12902335]"
/experiment="EXISTENCE:mutant phenotype:GO:0005381 iron
ion transmembrane transporter activity [PMID:12902335]"
/note="Iron transporter, mediates Fe2+ transport across
inner mito membrane; mitochondrial carrier family member;
active under low-iron conditions; may transport other
cations; MRS3 has a paralog, MRS4, that arose from the
whole genome duplication"
/codon_start=1
/product="Fe(2+) transporter"
/protein_id="NP_012402.1"
/db_xref="GeneID:853308"
/db_xref="SGD:S000003669"
/translation="MVENSSSNNSTRPIPAIPMDLPDYEALPTHAPLYHQLIAGAFAG
IMEHSVMFPIDALKTRIQSANAKSLSAKNMLSQISHISTSEGTLALWKGVQSVILGAG
PAHAVYFGTYEFCKKNLIDSSDTQTHHPFKTAISGACATTASDALMNPFDTIKQRIQL
NTSASVWQTTKQIYQSEGLAAFYYSYPTTLVMNIPFAAFNFVIYESSTKFLNPSNEYN
PLIHCLCGSISGSTCAAITTPLDCIKTVLQIRGSQTVSLEIMRKADTFSKAASAIYQV
YGWKGFWRGWKPRIVANMPATAISWTAYECAKHFLMTY"
gene <161914..>164166
/locus_tag="YJL132W"
/db_xref="GeneID:853309"
mRNA <161914..>164166
/locus_tag="YJL132W"
/product="uncharacterized protein"
/transcript_id="NM_001181565.1"
/db_xref="GeneID:853309"
CDS 161914..164166
/locus_tag="YJL132W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:11935221]"
/note="hypothetical protein; localizes to the membrane
fraction; possible Zap1p-regulated target gene induced by
zinc deficiency; YJL132W is a non-essential gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_012403.1"
/db_xref="GeneID:853309"
/db_xref="SGD:S000003668"
/translation="MSIISSWLLVSIICLTTSIVTKLQAAGVTTHLFYLTRGAPLSLK
ENYYPWLKAGSFFPDALYSCAPSNKDWSDFAEFTHWPNFLMIAVSYWQQKYGQNDRLR
GTHGSLALKSFLIGVFTHQIVDVSWHSLVTDYRMHGLLRVLSETEFDGDIETAHTFLD
VMGEFLTLNNVIRDSNNNENWDFLTRSDWKLPREEDLMEIIRNAGLSKEKLSYAELEF
CVKRGMAAAISEGYLFRSQRNQLLTNIYSTSPRANDLILNHWLGGQSNLVAMLQRCVP
FFETLFHDENTNEAQAEELRLCANLPPVSQKRINARPLVSSLKARKGNSHIVVSPMKS
FSDFGTSLTMGKFREDNKDYLAVSAPLEDTVGAIYIVPWDILTVARKEDFSILQPITA
MYGSKVGTYKASDVDYLLVSQPGTCTIDFYFKGVKILTIKDETTEEAHQLQFAVTGNF
YDDKIPDLVVSSPSYGANETGIATFIPGSSIISYLTNSDKYQVVDISTFKGVINLDGY
PMKIPFQHFGATIQISDTTDKQKLIYITCQSLGTVFVYSSNDLHDLSIPIYYITKNGV
IPAKDSDHVEWHIIPSKEHGMFGAAIYSWNFEGMSFVAVSQPMFDTVFIYIEKSGQIE
FFLKLVLKIKTKSDSIPDEFGSSLLFNDEEKKLYVSSPGSFDARGSIWKISMDELLKA
GNDPKRKTLLINNLRHLMLINPDKSSKGVSNFGNSMILGPQNHLIVGIPQYGYGNFDH
MQLTGRILVL"
gene complement(<164278..>165348)
/gene="AIM23"
/locus_tag="YJL131C"
/gene_synonym="MIF3"
/db_xref="GeneID:853310"
mRNA complement(<164278..>165348)
/gene="AIM23"
/locus_tag="YJL131C"
/gene_synonym="MIF3"
/product="Aim23p"
/transcript_id="NM_001181564.1"
/db_xref="GeneID:853310"
CDS complement(164278..165348)
/gene="AIM23"
/locus_tag="YJL131C"
/gene_synonym="MIF3"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14562095|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0097177
mitochondrial ribosome binding [PMID:30927524]"
/experiment="EXISTENCE:genetic interaction:GO:0070124
mitochondrial translational initiation [PMID:23954798]"
/note="Mitochondrial translation initiation factor 3 (IF3,
mIF3); evolutionarily conserved; interacts with
mitochondrial ribosomal small subunit; binds to E. coli
ribosomes in vitro; null mutant displays severe
respiratory growth defect and elevated frequency of
mitochondrial genome loss"
/codon_start=1
/product="Aim23p"
/protein_id="NP_012404.1"
/db_xref="GeneID:853310"
/db_xref="SGD:S000003667"
/translation="MLKVPLSDVLSQKMLFLKSFRYFHCTKYFSRDNASSTTDIFRNA
MKRKRELANLKEQSHGNVARNAAFPKEYIKRPKQVPRNATNRKKILITWSTGTDRAKE
AANSVVSEIFKKNHKGNIKVVDPTTHRIEASNIRYFAKGIDLDKVGLSIVNVEQIDNE
NQIPLVKIVESRVALKKYSDFLAKKKEKELMELGVLNKSYKNLVTDKKEDNLKHIKIS
WQIESDDLKRQKAHEIVSLLKKGNKVTLYLDDKNNINSNNWLENFEELDRSQKGEPPR
LPESVFQKRAAVLETLKEIVSEYANDPVLLGNMNSKMIMKLIPKDVKPQNNDKRALKE
LRKKERQEKLQKRIQRKKMNEM"
gene complement(<165723..>172367)
/gene="URA2"
/locus_tag="YJL130C"
/db_xref="GeneID:853311"
mRNA complement(<165723..>172367)
/gene="URA2"
/locus_tag="YJL130C"
/product="bifunctional carbamoylphosphate
synthetase/aspartate transcarbamylase"
/transcript_id="NM_001181563.2"
/db_xref="GeneID:853311"
CDS complement(165723..172367)
/gene="URA2"
/locus_tag="YJL130C"
/EC_number="2.1.3.2"
/EC_number="6.3.5.5"
/EC_number="3.5.1.2"
/EC_number="6.3.4.16"
/experiment="EXISTENCE:direct assay:GO:0004070 aspartate
carbamoyltransferase activity [PMID:10446140]"
/experiment="EXISTENCE:direct assay:GO:0004088
carbamoyl-phosphate synthase (glutamine-hydrolyzing)
activity [PMID:5776390]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11015727]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0006207 'de novo'
pyrimidine nucleobase biosynthetic process [PMID:181668]"
/experiment="EXISTENCE:direct assay:GO:0006541 glutamine
metabolic process [PMID:5651325]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:11921093]"
/experiment="EXISTENCE:direct assay:GO:0045984 negative
regulation of pyrimidine nucleobase metabolic process
[PMID:5651325]"
/experiment="EXISTENCE:mutant phenotype:GO:0004070
aspartate carbamoyltransferase activity [PMID:4550660]"
/experiment="EXISTENCE:mutant phenotype:GO:0006207 'de
novo' pyrimidine nucleobase biosynthetic process
[PMID:4550660]"
/note="Bifunctional carbamoylphosphate
synthetase/aspartate transcarbamylase; catalyzes the first
two enzymatic steps in the de novo biosynthesis of
pyrimidines; both activities are subject to feedback
inhibition by UTP"
/codon_start=1
/product="bifunctional carbamoylphosphate
synthetase/aspartate transcarbamylase"
/protein_id="NP_012405.2"
/db_xref="GeneID:853311"
/db_xref="SGD:S000003666"
/translation="MATIAPTAPITPPMESTGDRLVTLELKDGTVLQGYSFGAEKSVA
GELVFQTGMVGYPESVTDPSYEGQILVITYPLVGNYGVPDMHLRDELVEELPRYFESN
RIHIAGLVISHYTDEYSHYLAKSSLGKWLQNEGIPAVYGVDTRSLTKHLRDAGSMLGR
LSLEKSGSDRTISRSSSWRSAFDVPEWVDPNVQNLVSKVSINEPKLYVPPADNKHIEL
QTGPDGKVLRILAIDVGMKYNQIRCFIKRGVELKVVPWNYDFTKEDYDGLFISNGPGD
PSVLDDLSQRLSNVLEAKKTPVFGICLGHQLIARAAGASTLKLKFGNRGHNIPCTSTI
SGRCYITSQNHGFAVDVDTLTSGWKPLFVNANDDSNEGIYHSELPYFSVQFHPESTPG
PRDTEFLFDVFIQAVKEFKYTQVLKPIAFPGGLLEDNVKAHPRIEAKKVLVLGSGGLS
IGQAGEFDYSGSQAIKALKEEGIYTILINPNIATIQTSKGLADKVYFVPVTAEFVRKV
ILHERPDAIYVTFGGQTALSVGIAMKDEFEALGVKVLGTPIDTIITTEDRELFSNAID
EINEKCAKSQAANSVDEALAAVKEIGFPVIVRAAYALGGLGSGFANNEKELVDLCNVA
FSSSPQVLVEKSMKGWKEVEYEVVRDAFDNCITVCNMENFDPLGIHTGDSIVVAPSQT
LSDEDYNMLRTTAVNVIRHLGVVGECNIQYALNPVSKDYCIIEVNARLSRSSALASKA
TGYPLAYTAAKLGLNIPLNEVKNSVTKSTCACFEPSLDYCVVKMPRWDLKKFTRVSTE
LSSSMKSVGEVMSIGRTFEEAIQKAIRSTEYANLGFNETDLDIDIDYELNNPTDMRVF
AIANAFAKKGYSVDKVWEMTRIDKWFLNKLHDLVQFAEKISSFGTKEELPSLVLRQAK
QLGFDDRQIARFLDSNEVAIRRLRKEYGITPFVKQIDTVAAEFPAYTNYLYMTYNADS
HDLSFDDHGVMVLGSGVYRIGSSVEFDWCAVTAVRTLRANNIKTIMVNYNPETVSTDY
DEADRLYFETINLERVLDIYEIENSSGVVVSMGGQTSNNIAMTLHRENVKILGTSPDM
IDSAENRYKFSRMLDQIGVDQPAWKELTSMDEAESFAEKVGYPVLVRPSYVLSGAAMN
TVYSKNDLESYLNQAVEVSRDYPVVITKYIENAKEIEMDAVARNGELVMHVVSEHVEN
AGVHSGDATLIVPPQDLAPETVDRIVVATAKIGKALKITGPYNIQFIAKDNEIKVIEC
NVRASRSFPFISKVVGVNLIELATKAIMGLPLTPYPVEKLPDDYVAVKVPQFSFPRLA
GADPVLGVEMASTGEVATFGHSKYEAYLKSLLATGFKLPKKNILLSIGSYKEKQELLS
SVQKLYNMGYKLFATSGTADFLSEHGIAVQYLEVLNKDDDDQKSEYSLTQHLANNEID
LYINLPSANRFRRPASYVSKGYKTRRLAVDYSVPLVTNVKCAKLLIEAISRNITLDVS
ERDAQTSHRTITLPGLINIATYVPNASHVIKGPAELKETTRLFLESGFTYCQLMPRSI
SGPVITDVASLKAANSVSQDSSYTDFSFTIAGTAHNAHSVTQSASKVTALFLPLRELK
NKITAVAELLNQWPTEKQVIAEAKTADLASVLLLTSLQNRSIHITGVSNKEDLALIMT
VKAKDPRVTCDVNIYSLFIAQDDYPEAVFLPTKEDQEFFWNNLDSIDAFSVGALPVAL
ANVTGNKVDVGMGIKDSLPLLLAAVEEGKLTIDDIVLRLHDNPAKIFNIPTQDSVVEI
DLDYSFRRNKRWSPFNKDMNGGIERVVYNGETLVLSGELVSPGAKGKCIVNPSPASIT
ASAELQSTSAKRRFSITEEAIADNLDAAEDAIPEQPLEQKLMSSRPPRELVAPGAIQN
LIRSNNPFRGRHILSIKQFKRSDFHVLFAVAQELRAAVAREGVLDLMKGHVITTIFFE
PSTRTCSSFIAAMERLGGRIVNVNPLVSSVKKGETLQDTIRTLACYSDAIVMRHSEEM
SVHIAAKYSPVPIINGGNGSREHPTQAFLDLFTIREEIGTVNGITVTFMGDLKHGRTV
HSLCRLLMHYQVRINLVSPPELRLPEGLREELRKAGLLGVESIELTPHIISKTDVLYC
TRVQEERFNSPEEYARLKDTYIVDNKILAHAKENMAIMHPLPRVNEIKEEVDYDHRAA
YFRQMKYGLFVRMALLAMVMGVDM"
gene complement(<173599..>177306)
/gene="TRK1"
/locus_tag="YJL129C"
/db_xref="GeneID:853312"
mRNA complement(<173599..>177306)
/gene="TRK1"
/locus_tag="YJL129C"
/product="Trk1p"
/transcript_id="NM_001181562.1"
/db_xref="GeneID:853312"
CDS complement(173599..177306)
/gene="TRK1"
/locus_tag="YJL129C"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16166647]"
/experiment="EXISTENCE:direct assay:GO:0015079 potassium
ion transmembrane transporter activity [PMID:3043197]"
/experiment="EXISTENCE:direct assay:GO:0030007
intracellular potassium ion homeostasis [PMID:3043197]"
/experiment="EXISTENCE:direct assay:GO:0045121 membrane
raft [PMID:16166647]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0006874
intracellular calcium ion homeostasis [PMID:33640956]"
/note="Component of the Trk1p-Trk2p potassium transport
system; 180 kDa high affinity potassium transporter;
phosphorylated in vivo and interacts physically with the
phosphatase Ppz1p, suggesting Trk1p activity is regulated
by phosphorylation; TRK1 has a paralog, TRK2, that arose
from the whole genome duplication"
/codon_start=1
/product="Trk1p"
/protein_id="NP_012406.1"
/db_xref="GeneID:853312"
/db_xref="SGD:S000003665"
/translation="MHFRRTMSRVPTLASLEIRYKKSFGHKFRDFIALCGHYFAPVKK
YIFPSFIAVHYFYTISLTLITSILLYPIKNTRYIDTLFLAAGAVTQGGLNTVDINNLS
LYQQIVLYIVCCISTPIAVHSCLAFVRLYWFERYFDGIRDSSRRNFKMRRTKTILERE
LTARTMTKNRTGTQRTSYPRKQAKTDDFQEKLFSGEMVNRDEQDSVHSDQNSHDISRD
SSNNNTNHNGSSGSLDDFVKEDETDDNGEYQENNSYSTVGSSSNTVADESLNQKPKPS
SLRFDEPHSKQRPARVPSEKFAKRRGSRDISPADMYRSIMMLQGKHEATAEDEGPPLV
IGSPADGTRYKSNVNKLKKATGINGNKIKIRDKGNESNTDQNSVSSEANSTASVSDES
SLHTNFGNKVPSLRTNTHRSNSGPIAITDNAETDKKHGPSIQFDITKPPRKISKRVST
FDDLNPKSSVLYRKKASKKYLMKHFPKARRIRQQIKRRLSTGSIEKNSSNNVSDRKPI
TDMDDDDDDDDNDGDNNEEYFADNESGDEDERVQQSEPHSDSELKSHQQQQEKHQLQQ
NLHRMYKTKSFDDNRSRAVPMERSRTIDMAEAKDLNELARTPDFQKMVYQNWKAHHRK
KPNFRKRGWNNKIFEHGPYASDSDRNYPDNSNTGNSILHYAESILHHDGSHKNGSEEA
SSDSNENIYSTNGGSDHNGLNNYPTYNDDEEGYYGLHFDTDYDLDPRHDLSKGSGKTY
LSWQPTIGRNSNFLGLTRAQKDELGGVEYRAIKLLCTILVVYYVGWHIVAFVMLVPWI
ILKKHYSEVVRDDGVSPTWWGFWTAMSAFNDLGLTLTPNSMMSFNKAVYPLIVMIWFI
IIGNTGFPILLRCIIWIMFKISPDLSQMRESLGFLLDHPRRCFTLLFPKAATWWLLLT
LAGLNITDWILFIILDFGSTVVKSLSKGYRVLVGLFQSVSTRTAGFSVVDLSQLHPSI
QVSYMLMMYVSVLPLAISIRRTNVYEEQSLGLYGDMGGEPEDTDTEDDGNDEDDDEEN
ESHEGQSSQRSSSNNNNNNNRKKKKKKKTENPNEISTKSFIGAHLRKQLSFDLWFLFL
GLFIICICEGDKIKDVQEPNFNIFAILFEIVSAYGTVGLSLGYPDTNQSFSRQFTTLS
KLVIIAMLIRGKNRGLPYSLDRAIILPSDRLEHIDHLEGMKLKRQARTNTEDPMTEHF
KRSFTDVKHRWGALKRKTTHSRNPKRSSTTL"
gene complement(<178097..>180103)
/gene="PBS2"
/locus_tag="YJL128C"
/gene_synonym="HOG4; SFS4; SSK4"
/db_xref="GeneID:853313"
mRNA complement(<178097..>180103)
/gene="PBS2"
/locus_tag="YJL128C"
/gene_synonym="HOG4; SFS4; SSK4"
/product="mitogen-activated protein kinase kinase PBS2"
/transcript_id="NM_001181561.2"
/db_xref="GeneID:853313"
CDS complement(178097..180103)
/gene="PBS2"
/locus_tag="YJL128C"
/gene_synonym="HOG4; SFS4; SSK4"
/EC_number="2.7.12.2"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:9755161]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:10980703]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:10980703]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405]"
/experiment="EXISTENCE:genetic interaction:GO:0038066
p38MAPK cascade [PMID:7624781]"
/experiment="EXISTENCE:genetic interaction:GO:0071474
cellular hyperosmotic response [PMID:7624781]"
/experiment="EXISTENCE:mutant phenotype:GO:0004708 MAP
kinase kinase activity [PMID:7624781]"
/experiment="EXISTENCE:mutant phenotype:GO:0006606 protein
import into nucleus [PMID:15707964]"
/experiment="EXISTENCE:mutant phenotype:GO:0006972
hyperosmotic response [PMID:7681220]"
/experiment="EXISTENCE:mutant phenotype:GO:0007015 actin
filament organization [PMID:7941729]"
/experiment="EXISTENCE:mutant phenotype:GO:0007231
osmosensory signaling pathway [PMID:7624781]"
/experiment="EXISTENCE:mutant phenotype:GO:0016239
positive regulation of macroautophagy [PMID:40524543]"
/experiment="EXISTENCE:mutant phenotype:GO:0038066 p38MAPK
cascade [PMID:15707964]"
/experiment="EXISTENCE:physical interaction:GO:0005078
MAP-kinase scaffold activity
[PMID:15200959|PMID:9180081|PMID:15200958]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:14685261]"
/experiment="EXISTENCE:physical interaction:GO:0007232
osmosensory signaling pathway via Sho1 osmosensor
[PMID:15200958]"
/note="MAP kinase kinase of the HOG signaling pathway;
activated under severe osmotic stress; regulates
late-stage macroautophagy after autophagosome closure and
mitophagy; plays a role in regulating Ty1 transposition"
/codon_start=1
/product="mitogen-activated protein kinase kinase PBS2"
/protein_id="NP_012407.2"
/db_xref="GeneID:853313"
/db_xref="SGD:S000003664"
/translation="MEDKFANLSLHEKTGKSSIQLNEQTGSDNGSAVKRTSSTSSHYN
NINADLHARVKAFQEQRALKRSASVGSNQSEQDKGSSQSPKHIQQIVNKPLPPLPVAG
SSKVSQRMSSQVVQASSKSTLKNVLDNQETQNITDVNINIDTTKITATTIGVNTGLPA
TDITPSVSNTASATHKAQLLNPNRRAPRRPLSTQHPTRPNVAPHKAPAIINTPKQSLS
ARRGLKLPPGGMSLKMPTKTAQQPQQFAPSPSNKKHIETLSNSKVVEGKRSNPGSLIN
GVQSTSTSSSTEGPHDTVGTTPRTGNSNNSSNSGSSGGGGLFANFSKYVDIKSGSLNF
AGKLSLSSKGIDFSNGSSSRITLDELEFLDELGHGNYGNVSKVLHKPTNVIMATKEVR
LELDEAKFRQILMELEVLHKCNSPYIVDFYGAFFIEGAVYMCMEYMDGGSLDKIYDES
SEIGGIDEPQLAFIANAVIHGLKELKEQHNIIHRDVKPTNILCSANQGTVKLCDFGVS
GNLVASLAKTNIGCQSYMAPERIKSLNPDRATYTVQSDIWSLGLSILEMALGRYPYPP
ETYDNIFSQLSAIVDGPPPRLPSDKFSSDAQDFVSLCLQKIPERRPTYAALTEHPWLV
KYRNQDVHMSEYITERLERRNKILRERGENGLSKNVPALHMGGL"
gene complement(<181551..>181709)
/gene="MCO6"
/locus_tag="YJL127C-B"
/gene_synonym="YJL127C-A"
/db_xref="GeneID:1466466"
mRNA complement(<181551..>181709)
/gene="MCO6"
/locus_tag="YJL127C-B"
/gene_synonym="YJL127C-A"
/product="Mco6p"
/transcript_id="NM_001184532.1"
/db_xref="GeneID:1466466"
CDS complement(181551..181709)
/gene="MCO6"
/locus_tag="YJL127C-B"
/gene_synonym="YJL127C-A"
/experiment="EXISTENCE:direct assay:GO:0001401 SAM complex
[PMID:38377000]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:28658629]"
/experiment="EXISTENCE:genetic interaction:GO:0070096
mitochondrial outer membrane translocase complex assembly
[PMID:38377000]"
/experiment="EXISTENCE:mutant phenotype:GO:0070096
mitochondrial outer membrane translocase complex assembly
[PMID:38377000]"
/note="Mitochondrial outer membrane protein protein;
co-assembles with Mdm10p into the mitochondrial sorting
and assembly machinery (SAM) complex and cooperates with
Mdm10p in the assembly of the translocase of the outer
membrane (TOM) complex; identified based on homology to
the filamentous fungus, Ashbya gossypii; SWAT-GFP and
seamless-GFP fusion proteins localize to the mitochondria"
/codon_start=1
/product="Mco6p"
/protein_id="NP_878103.1"
/db_xref="GeneID:1466466"
/db_xref="SGD:S000028522"
/translation="MIFFFNQIRSIFTALHTPTQQIQLSRRAFFQFLGYLGSCVVISL
AAQSKYVQ"
gene complement(<182298..>184220)
/gene="SPT10"
/locus_tag="YJL127C"
/gene_synonym="CRE1; SUD1"
/db_xref="GeneID:853315"
mRNA complement(<182298..>184220)
/gene="SPT10"
/locus_tag="YJL127C"
/gene_synonym="CRE1; SUD1"
/product="Spt10p"
/transcript_id="NM_001181560.1"
/db_xref="GeneID:853315"
CDS complement(182298..184220)
/gene="SPT10"
/locus_tag="YJL127C"
/gene_synonym="CRE1; SUD1"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:16199888]"
/experiment="EXISTENCE:direct assay:GO:1990841
promoter-specific chromatin binding [PMID:15882620]"
/experiment="EXISTENCE:genetic interaction:GO:0030466
silent mating-type cassette heterochromatin formation
[PMID:21057056]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:17526727]"
/experiment="EXISTENCE:mutant phenotype:GO:0000183 rDNA
heterochromatin formation [PMID:21057056]"
/experiment="EXISTENCE:mutant phenotype:GO:0006281 DNA
repair [PMID:17078097]"
/experiment="EXISTENCE:mutant phenotype:GO:0010507
negative regulation of autophagy [PMID:26649943]"
/experiment="EXISTENCE:mutant phenotype:GO:0031509
subtelomeric heterochromatin formation [PMID:21057056]"
/note="Histone H3 acetylase with a role in transcriptional
regulation; sequence-specific activator of histone genes,
binds specifically and cooperatively to pairs of UAS
elements in core histone promoters, functions at or near
TATA box; involved in S phase-specific acetylation of
H3K56 at histone promoters, which is required for
recruitment of SWI/SNF nucleosome remodeling complex and
subsequent transcription"
/codon_start=1
/product="Spt10p"
/protein_id="NP_012408.1"
/db_xref="GeneID:853315"
/db_xref="SGD:S000003663"
/translation="MLNQHTSSVPDDEHLQMAHQNSSSEVRNEAAVPDQLLTPLQPYT
ILLKDGETIATMYPIPAYPDLLPLGLLNFLLDEFNMEVEKGDSFPYYETLSLEEFKNV
WFHNDGHVCIMVLGEIPELDYSMDTEADTNDNFGTEIETTKHTTQYKKRKERRNLNLS
MQWEKQCLGIFDLKPAYPGRSAHVVTGTFLVNAGIRGKGIGKTLMETFIEWSKKLGFT
SSFFPLIYGTNVGIRRILEGLNFRRIGKLPEAGILKGFDVPVDSFMYGKEFTHITKSI
DLLRDPQKSIEIGKYERLKHFLETGKYPLHCDRNEKARLRVLSKTHSVLNGKLMTKGK
EIIYDTDQQIQIALEIHLMEHLGINKVTSKIGEKYHWRGIKSTVSEVISRCQKCKMRY
KDGTGVIIEQKRAVKQAHMLPTQHIETINNPRKSKKHDNALLGQAINFPQNIISSTLN
DVEGEPTPPDTNIVQPTFQNATNSPATTAEANEANKRSEFLSSIQSTPLLDDEQSMNS
FNRFVEEENSRKRRKYLDVASNGIVPHLTNNESQDHANPVNRDERDMNHSVPDLDRND
HTIMNDAMLSLEDNVMAALEMVQKEQQQKINHRGEDVTGQQIDLNNSEGNENSVTKIV
NNESNTFTEHNSNIYY"
gene <184501..>185424
/gene="NIT2"
/locus_tag="YJL126W"
/db_xref="GeneID:853316"
mRNA <184501..>185424
/gene="NIT2"
/locus_tag="YJL126W"
/product="putative hydrolase"
/transcript_id="NM_001181559.1"
/db_xref="GeneID:853316"
CDS 184501..185424
/gene="NIT2"
/locus_tag="YJL126W"
/EC_number="3.5.1.128"
/experiment="EXISTENCE:direct assay:GO:0043605 amide
catabolic process [PMID:28373563]"
/experiment="EXISTENCE:direct assay:GO:0110050 deaminated
glutathione amidase activity [PMID:28373563]"
/note="Nit protein; one of two proteins in S. cerevisiae
with similarity to the Nit domain of NitFhit from fly and
worm and to the mouse and human Nit protein which
interacts with the Fhit tumor suppressor; nitrilase
superfamily member"
/codon_start=1
/product="putative hydrolase"
/protein_id="NP_012409.1"
/db_xref="GeneID:853316"
/db_xref="SGD:S000003662"
/translation="MTSKLKRVAVAQLCSSADLTKNLKVVKELISEAIQKKADVVFLP
EASDYLSQNPLHSRYLAQKSPKFIRQLQSSITDLVRDNSRNIDVSIGVHLPPSEQDLL
EGNDRVRNVLLYIDHEGKILQEYQKLHLFDVDVPNGPILKESKSVQPGKAIPDIIESP
LGKLGSAICYDIRFPEFSLKLRSMGAEILCFPSAFTIKTGEAHWELLGRARAVDTQCY
VLMPGQVGMHDLSDPEWEKQSHMSALEKSSRRESWGHSMVIDPWGKIIAHADPSTVGP
QLILADLDRELLQEIRNKMPLWNQRRDDLFH"
gene complement(<185528..>186679)
/gene="GCD14"
/locus_tag="YJL125C"
/gene_synonym="TRM61"
/db_xref="GeneID:853317"
mRNA complement(<185528..>186679)
/gene="GCD14"
/locus_tag="YJL125C"
/gene_synonym="TRM61"
/product="tRNA 1-methyladenosine methyltransferase subunit
GCD14"
/transcript_id="NM_001181558.1"
/db_xref="GeneID:853317"
CDS complement(185528..186679)
/gene="GCD14"
/locus_tag="YJL125C"
/gene_synonym="TRM61"
/EC_number="2.1.1.220"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9851972]"
/experiment="EXISTENCE:direct assay:GO:0030488 tRNA
methylation [PMID:10779558]"
/experiment="EXISTENCE:direct assay:GO:0031515 tRNA (m1A)
methyltransferase complex [PMID:10779558]"
/experiment="EXISTENCE:direct assay:GO:0160107 tRNA
(adenine(58)-N1)-methyltransferase activity
[PMID:10779558]"
/experiment="EXISTENCE:mutant phenotype:GO:0160107 tRNA
(adenine(58)-N1)-methyltransferase activity
[PMID:10779558]"
/experiment="EXISTENCE:physical interaction:GO:0031515
tRNA (m1A) methyltransferase complex [PMID:10779558]"
/note="Subunit of tRNA (1-methyladenosine)
methyltransferase; required, along with Gcd10p, for the
modification of the adenine at position 58 in tRNAs,
especially tRNAi-Met; first identified as a negative
regulator of GCN4 expression"
/codon_start=1
/product="tRNA 1-methyladenosine methyltransferase subunit
GCD14"
/protein_id="NP_012410.1"
/db_xref="GeneID:853317"
/db_xref="SGD:S000003661"
/translation="MSTNCFSGYKDLIKEGDLTLIWVSRDNIKPVRMHSEEVFNTRYG
SFPHKDIIGKPYGSQIAIRTKGSNKFAFVHVLQPTPELWTLSLPHRTQIVYTPDSSYI
MQRLNCSPHSRVIEAGTGSGSFSHAFARSVGHLFSFEFHHIRYEQALEEFKEHGLIDD
NVTITHRDVCQGGFLIKKGDTTSYEFGNNETAASLNANVVFLDLPAPWDAIPHLDSVI
SVDEKVGLCCFSPCIEQVDKTLDVLEKYGWTDVEMVEIQGRQYESRRQMVRSLNDALE
RLRDIKRHKLQGVERRKRMFNNTIDSNDEKVGKRNEDGVPLTEKAKFNPFGKGSRIKE
GDSNYKWKEVTKMEAEIKSHTSYLTFAFKVVNRSRDDEKVNEILRSTEK"
gene complement(<187126..>187644)
/gene="LSM1"
/locus_tag="YJL124C"
/gene_synonym="SPB8"
/db_xref="GeneID:853318"
mRNA complement(<187126..>187644)
/gene="LSM1"
/locus_tag="YJL124C"
/gene_synonym="SPB8"
/product="Lsm1p"
/transcript_id="NM_001181557.1"
/db_xref="GeneID:853318"
CDS complement(187126..187644)
/gene="LSM1"
/locus_tag="YJL124C"
/gene_synonym="SPB8"
/experiment="EXISTENCE:direct assay:GO:0000932 P-body
[PMID:18611963|PMID:12730603]"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:23706738]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:23706738]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:23706738|PMID:18029398|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:1990726 Lsm1-7-Pat1
complex [PMID:24139796]"
/experiment="EXISTENCE:mutant phenotype:GO:0000288
nuclear-transcribed mRNA catabolic process,
deadenylation-dependent decay [PMID:10747033]"
/experiment="EXISTENCE:mutant phenotype:GO:0000290
deadenylation-dependent decapping of nuclear-transcribed
mRNA [PMID:10761922]"
/experiment="EXISTENCE:mutant phenotype:GO:0000932 P-body
[PMID:12730603]"
/note="Lsm (Like Sm) protein; forms heteroheptameric
complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and
Lsm7p) involved in degradation of cytoplasmic mRNAs; also
enters the nucleus and positively regulates transcription
initiation; unlike most Sm-like proteins, Lsm1p requires
both its SM-domain and C-terminal domain for RNA-binding;
binds to mRNAs under glucose starvation, most often in the
3' UTR; forms cytoplasmic foci upon DNA replication
stress"
/codon_start=1
/product="Lsm1p"
/protein_id="NP_012411.1"
/db_xref="GeneID:853318"
/db_xref="SGD:S000003660"
/translation="MSANSKDRNQSNQDAKRQQQNFPKKISEGEADLYLDQYNFTTTA
AIVSSVDRKIFVLLRDGRMLFGVLRTFDQYANLILQDCVERIYFSEENKYAEEDRGIF
MIRGENVVMLGEVDIDKEDQPLEAMERIPFKEAWLTKQKNDEKRFKEETHKGKKMARH
GIVYDFHKSDMY"
gene complement(<188004..>189440)
/gene="MTC1"
/locus_tag="YJL123C"
/db_xref="GeneID:853319"
mRNA complement(<188004..>189440)
/gene="MTC1"
/locus_tag="YJL123C"
/product="DUF5427 domain-containing protein MTC1"
/transcript_id="NM_001181556.1"
/db_xref="GeneID:853319"
CDS complement(188004..189440)
/gene="MTC1"
/locus_tag="YJL123C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:31323700]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:14562095]"
/note="hypothetical protein that may interact with
ribosomes; green fluorescent protein (GFP)-fusion protein
localizes to the cytoplasm and to COPI-coated vesicles
(early Golgi); mtc1 is synthetically lethal with cdc13-1"
/codon_start=1
/product="DUF5427 domain-containing protein MTC1"
/protein_id="NP_012412.1"
/db_xref="GeneID:853319"
/db_xref="SGD:S000003659"
/translation="MSENKNSEAEDVFEFLDSLPEAKNGGKMVNTDVKGSQEGVKGGS
NSVAGKTGNDGKKGDDDIFEFLEELEKSNLSLTDKKGVEKKAPSESVNNKAQDEKVEE
SKENKNSEQDAHGKEKEPQQQEKEEEEEEEEEEEEEEEETPLHDPIASISNWWSSSGS
AKVSSIWNKTAEQASQIKNRLAQEQLDLTSKINTSTITEIARNLQKIVVGETEEVLRI
HLVHDLVNYPSLQYNIESKFDQVLSSQVEGGIRIFVDEWGHPNNNGITPVEKKPSVAD
GELGNSKKKLQFNLFDGKVTDGEKLAFANLENAVKLFNTAHEEYQKQQKEADATPDDD
RSSISSNSNKISDLFISILPIAIPQKQKDADGDFQVTDSNTPGNFNFTLVLKDITNDI
TTITRSQGFPVKWVNWLEGSVEKTGSTASEERNKSYDQKKQKESEDEDEDDEIIDPSE
WVKEWIEDGLSLSFGVMAQNYVIDRMGL"
gene <189716..>190243
/gene="ALB1"
/locus_tag="YJL122W"
/db_xref="GeneID:853320"
mRNA <189716..>190243
/gene="ALB1"
/locus_tag="YJL122W"
/product="Alb1p"
/transcript_id="NM_001181555.1"
/db_xref="GeneID:853320"
CDS 189716..190243
/gene="ALB1"
/locus_tag="YJL122W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:16651379|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:16651379]"
/experiment="EXISTENCE:genetic interaction:GO:0042273
ribosomal large subunit biogenesis [PMID:16651379]"
/experiment="EXISTENCE:physical interaction:GO:0042273
ribosomal large subunit biogenesis [PMID:16651379]"
/note="Shuttling pre-60S factor; involved in the
biogenesis of ribosomal large subunit; interacts directly
with Arx1p; responsible for Tif6p recycling defects in
absence of Rei1p"
/codon_start=1
/product="Alb1p"
/protein_id="NP_012413.1"
/db_xref="GeneID:853320"
/db_xref="SGD:S000003658"
/translation="MPSKNSINRPKLTSNLHHKVHSLNKKRAQRERAGLLKPARSSVN
SKSGEIKSVALDLYFQNKKNESQNSTAVTLQNASSSPASITTRTLSKKRAKKIERNLK
YATQRKLLVDASAKLEDEMDIDLDGGKKVKENEKKSSLTLVKEALWSVIDDTASQGLI
IENGQGTTLGGPFFP"
gene complement(<190374..>191090)
/gene="RPE1"
/locus_tag="YJL121C"
/gene_synonym="EPI1; POS18"
/db_xref="GeneID:853322"
mRNA complement(<190374..>191090)
/gene="RPE1"
/locus_tag="YJL121C"
/gene_synonym="EPI1; POS18"
/product="ribulose-phosphate 3-epimerase RPE1"
/transcript_id="NM_001181554.1"
/db_xref="GeneID:853322"
CDS complement(190374..191090)
/gene="RPE1"
/locus_tag="YJL121C"
/gene_synonym="EPI1; POS18"
/EC_number="5.1.3.1"
/experiment="EXISTENCE:direct assay:GO:0004750
D-ribulose-phosphate 3-epimerase activity [PMID:8929392]"
/experiment="EXISTENCE:mutant phenotype:GO:0004750
D-ribulose-phosphate 3-epimerase activity [PMID:8929392]"
/experiment="EXISTENCE:mutant phenotype:GO:0006098
pentose-phosphate shunt [PMID:8929392]"
/note="D-ribulose-5-phosphate 3-epimerase; catalyzes a
reaction in the non-oxidative part of the
pentose-phosphate pathway; mutants are sensitive to
oxidative stress"
/codon_start=1
/product="ribulose-phosphate 3-epimerase RPE1"
/protein_id="NP_012414.1"
/db_xref="GeneID:853322"
/db_xref="SGD:S000003657"
/translation="MVKPIIAPSILASDFANLGCECHKVINAGADWLHIDVMDGHFVP
NITLGQPIVTSLRRSVPRPGDASNTEKKPTAFFDCHMMVENPEKWVDDFAKCGADQFT
FHYEATQDPLHLVKLIKSKGIKAACAIKPGTSVDVLFELAPHLDMALVMTVEPGFGGQ
KFMEDMMPKVETLRAKFPHLNIQVDGGLGKETIPKAAKAGANVIVAGTSVFTAADPHD
VISFMKEEVSKELRSRDLLD"
gene <191639..>192298
/locus_tag="YJL118W"
/db_xref="GeneID:853323"
mRNA <191639..>192298
/locus_tag="YJL118W"
/product="uncharacterized protein"
/transcript_id="NM_001181551.1"
/db_xref="GeneID:853323"
CDS 191639..192298
/locus_tag="YJL118W"
/note="hypothetical protein; may interact with ribosomes,
based on co-purification experiments; YJL118W is a
non-essential gene; deletion enhances the toxicity of
heterologously expressed human alpha-synuclein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_012417.1"
/db_xref="GeneID:853323"
/db_xref="SGD:S000003654"
/translation="MASCFSVSLLARVAVVEPIRVQLWLNVVNCMIESSMHQCPPRDR
HFFSSSRPILLIRRSVSTVYRFVASRTTQVLRAAKTVVKWFIIVDPLINSILINYLID
RLCTLGHAVLRVKKRKTEERQPCSPIIQHTHVKRRKRPRLRIVAIKRKRRRRRPHRIE
RPLSNMYPIMEIQMVAVPLALPSPTALVHYQQQQQQLPQHHPWYDLSLSEEALSTCCC
S"
gene <192531..>193466
/gene="PHO86"
/locus_tag="YJL117W"
/db_xref="GeneID:853325"
mRNA <192531..>193466
/gene="PHO86"
/locus_tag="YJL117W"
/product="Pho86p"
/transcript_id="NM_001181550.1"
/db_xref="GeneID:853325"
CDS 192531..193466
/gene="PHO86"
/locus_tag="YJL117W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:10655492|PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0006457 protein
folding [PMID:15623581]"
/experiment="EXISTENCE:mutant phenotype:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:10655492]"
/experiment="EXISTENCE:mutant phenotype:GO:0010966
regulation of phosphate transport [PMID:10655492]"
/experiment="EXISTENCE:mutant phenotype:GO:0051082
unfolded protein binding [PMID:15623581]"
/note="Endoplasmic reticulum (ER) resident protein;
required for ER exit of the high-affinity phosphate
transporter Pho84p, specifically required for packaging of
Pho84p into COPII vesicles; protein abundance increases in
response to DNA replication stress"
/codon_start=1
/product="Pho86p"
/protein_id="NP_012418.1"
/db_xref="GeneID:853325"
/db_xref="SGD:S000003653"
/translation="MAVQQRKKKEGRKSDKNAPSVPQVDASLDKPLDIDAPPTIYSVN
LKPEYGTAALNLSADFIRQEQALANKYLFFHPVILVVLTIGLLIYLTPRIVFPIRNTG
SVAGWFYQLARINKKVVLSGLVFTAIGASFLFTLLSRVSDSYFKSKINQLVGSKGEKV
FGINLNDLVARHETKDPVVNNTHIIVYRETPIALISLAPNMTLSTDENLVMSVTTVGC
RRVYVKSGIIEDLIDWAMLHSKNIRSSGKYGETMKLLIDVYSFDSTLKEILKKKGFTY
IQSIRVSENRLLGGLFGVKKELWGLQFHFKAEHKD"
gene complement(<193860..>194873)
/gene="NCA3"
/locus_tag="YJL116C"
/db_xref="GeneID:853326"
mRNA complement(<193860..>194873)
/gene="NCA3"
/locus_tag="YJL116C"
/product="SUN family protein NCA3"
/transcript_id="NM_001181549.1"
/db_xref="GeneID:853326"
CDS complement(193860..194873)
/gene="NCA3"
/locus_tag="YJL116C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0042776
proton motive force-driven mitochondrial ATP synthesis
[PMID:7586026]"
/note="Protein involved in mitochondrion organization;
functions with Nca2p to regulate mitochondrial expression
of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP
synthase; SWAT-GFP, seamless-GFP and mCherry fusion
proteins localize to the vacuole; member of the SUN
family; expression induced in cells treated with the
mycotoxin patulin; NCA3 has a paralog, UTH1, that arose
from the whole genome duplication"
/codon_start=1
/product="SUN family protein NCA3"
/protein_id="NP_012419.1"
/db_xref="GeneID:853326"
/db_xref="SGD:S000003652"
/translation="MKISAALILSSLSSVAFSAPAPAPADSHHEDHHKDEKPAVVTVT
QYIDSNAATSTVESAATTTTLSSSEKDTSEQKRDGGFQDGTVKCSDFPSVNGIVSLDW
LGFGGWASVMDMDANTSSECKDGYYCSYACEPGMSKTQWPSDQPSDGKSVGGLYCKNG
YLYRTNTDTSDLCSTDETSAKAINKKSDSIALCRTDYPGSENMVIPTVVDGGDSQPIS
VVDEDTYYQWQGKKTSAQYYINNAGVSAEDGCIWGTSGSDVGNWAPLVLGAGSTNGET
YLSLIPNPNSNQAANFNVKIVASDGANVQGSCAYEDGSFTGDGSDGCTVSVLSGSAEF
VFY"
gene <196287..>197126
/gene="ASF1"
/locus_tag="YJL115W"
/gene_synonym="CIA1"
/db_xref="GeneID:853327"
mRNA <196287..>197126
/gene="ASF1"
/locus_tag="YJL115W"
/gene_synonym="CIA1"
/product="nucleosome assembly factor ASF1"
/transcript_id="NM_001181548.1"
/db_xref="GeneID:853327"
CDS 196287..197126
/gene="ASF1"
/locus_tag="YJL115W"
/gene_synonym="CIA1"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476|PMID:11404324]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0006325 chromatin
organization [PMID:16303565]"
/experiment="EXISTENCE:direct assay:GO:0006335 DNA
replication-dependent chromatin assembly [PMID:11412995]"
/experiment="EXISTENCE:direct assay:GO:0032968 positive
regulation of transcription elongation by RNA polymerase
II [PMID:22308335]"
/experiment="EXISTENCE:genetic interaction:GO:0030466
silent mating-type cassette heterochromatin formation
[PMID:15840725]"
/experiment="EXISTENCE:genetic interaction:GO:0031509
subtelomeric heterochromatin formation [PMID:15840725]"
/experiment="EXISTENCE:mutant phenotype:GO:0001932
regulation of protein phosphorylation [PMID:27222517]"
/experiment="EXISTENCE:mutant phenotype:GO:0006337
nucleosome disassembly [PMID:16678113]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:19620280]"
/experiment="EXISTENCE:mutant phenotype:GO:0010468
regulation of gene expression [PMID:26941319]"
/experiment="EXISTENCE:mutant phenotype:GO:0033554
cellular response to stress [PMID:19620280]"
/experiment="EXISTENCE:mutant phenotype:GO:0042393 histone
binding [PMID:15840725]"
/note="Nucleosome assembly factor; involved in chromatin
assembly, disassembly; required for recovery after DSB
repair; role in H3K56 acetylation required for expression
homeostasis, buffering mRNA synthesis rate against gene
dosage changes in S phase; anti-silencing protein,
derepresses silent loci when overexpressed; role in
regulating Ty1 transposition; relocalizes to cytosol under
hypoxia; growth defect of asf1 null is functionally
complemented by either human ASF1A or ASF1B"
/codon_start=1
/product="nucleosome assembly factor ASF1"
/protein_id="NP_012420.1"
/db_xref="GeneID:853327"
/db_xref="SGD:S000003651"
/translation="MSIVSLLGIKVLNNPAKFTDPYEFEITFECLESLKHDLEWKLTY
VGSSRSLDHDQELDSILVGPVPVGVNKFVFSADPPSAELIPASELVSVTVILLSCSYD
GREFVRVGYYVNNEYDEEELRENPPAKVQVDHIVRNILAEKPRVTRFNIVWDNENEGD
LYPPEQPGVDDEEEEDDEEEDDDEDDEDDEDDDQEDGEGEAEEAAEEEEEEEEKTEDN
ETNLEEEEEDIENSDGDEEEGEEEVGSVDKNEDGNDKKRRKIEGGSTDIESTPKDAAR
STN"
gene complement(197313..197385)
/locus_tag="YNCJ0005C"
/db_xref="GeneID:853328"
tRNA complement(197313..197385)
/locus_tag="YNCJ0005C"
/product="tRNA-Ala"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE
analysis; one of 11 nuclear tRNA genes containing the
tDNA-anticodon AGC (converted to IGC in the mature tRNA),
decodes GCU and GCC codons into alanine, one of 16 nuclear
tRNAs for alanine"
/db_xref="GeneID:853328"
/db_xref="SGD:S000006514"
repeat_region 197401..197494
/note="Ty3 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007035"
mobile_element 197545..203768
/note="YJLWTy4-1; Ty4 element, LTR retrotransposon of the
Copia (Pseudoviridae) group; contains co-transcribed genes
TYA Gag and TYB Pol, encoding proteins involved in
structure and function of virus-like particles, flanked by
two direct repeats"
/mobile_element_type="retrotransposon:YJLWTy4-1"
/db_xref="SGD:S000007039"
repeat_region 197545..197915
/note="Ty4 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007036"
gene <197915..>198595
/locus_tag="YJL114W"
/db_xref="GeneID:853329"
mRNA <197915..>198595
/locus_tag="YJL114W"
/product="gag protein"
/transcript_id="NM_001181547.2"
/db_xref="GeneID:853329"
CDS 197915..198595
/locus_tag="YJL114W"
/note="Retrotransposon TYA Gag gene co-transcribed with
TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is
a nucleocapsid protein that is the structural constituent
of virus-like particles (VLPs); similar to retroviral Gag"
/codon_start=1
/product="gag protein"
/protein_id="NP_012422.2"
/db_xref="GeneID:853329"
/db_xref="SGD:S000003650"
/translation="MATPVRGETRNVIDDNISARIQSKVKTNDTVRQTPSSLRKVSIK
DEQVRQYQRNLNRFKTILNGLKAEEEKLSEADDIQMLAEKLLKLGETIDKVENRIVDL
VEKIQLLETNENNNILHEHIDATGTYYLFDTLTSTNKRFYPKDCVFDYRTNNVENIPI
LLNNFKKFIKKYQFDDVFENDIIEIDPRENEILCKIIKEGLGESLDIMNTNTTDIFRI
IDGLKKQI"
gene <198678..>203325
/locus_tag="YJL113W"
/db_xref="GeneID:853330"
mRNA <198678..>203325
/locus_tag="YJL113W"
/product="gag-pol fusion protein"
/transcript_id="NM_001181546.3"
/db_xref="GeneID:853330"
CDS join(198678..198998,199000..203325)
/locus_tag="YJL113W"
/EC_number="2.7.7.7"
/EC_number="2.7.7.49"
/EC_number="3.1.26.4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9448009]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/ribosomal_slippage
/note="Retrotransposon TYA Gag and TYB Pol genes;
transcribed/translated as one unit; polyprotein is
processed to make a nucleocapsid-like protein (Gag),
reverse transcriptase (RT), protease (PR), and integrase
(IN); similar to retroviral genes"
/codon_start=1
/product="gag-pol fusion protein"
/protein_id="NP_012421.2"
/db_xref="GeneID:853330"
/db_xref="SGD:S000003649"
/translation="MNKLQKLVLMEKWIFSKCCQDCPNLKDYLQEAIMGTLHESLRNS
VKQRLYNIPHDVGIDHEEFLINTVIETVIDLSPIADDQIENSCMYCKSVFHCSINCKK
KPNRELGLTRPISQKPIIYKVHRDNNHLSPVQNEQKSWNKTQKRSNKVYNSKKLVIID
TGSGVNITNDKTLLHNYEDSNRSTRFFGIGKNSSVSVKGYGYIKIKNGHNNTDNKCLL
TYYVPEEESTIISCYDLAKKTKMVLSRKYTRLGNKIIKIKTKIVNGVIHVKMNELIER
PSDDSKINAIKPTSSPGFKLNKRSITLEDAHKRMGHTGIQQIENSIKHNHYEESLDLI
KEPNEFWCQTCKISKATKRNHYTGSMNNHSTDHEPGSSWCMDIFGPVSSSNADTKRYM
LIMVDNNTRYCMTSTHFNKNAETILAQVRKNIQYVETQFDRKVREINSDRGTEFTNDQ
IEEYFISKGIHHILTSTQDHAANGRAERYIRTIITDATTLLRQSNLRVKFWEYAVTSA
TNIRNYLEHKSTGKLPLKAISRQPVTVRLMSFLPFGEKGIIWNHNHKKLKPSGLPSII
LCKDPNSYGYKFFIPSKNKIVTSDNYTIPNYTMDGRVRNTQNINKSHQFSSDNDDEED
QIETVTNLCEALENYEDDNKPITRLEDLFTEEELSQIDSNAKYPSPSNNLEGDLDYVF
SDVEESGDYDVESELSTTNNSISTDKNKILSNKDFNSELASTEISISGIDKKGLINTS
HIDEDKYDEKVHRIPSIIQEKLVGSKNTIKINDENKISDRIRSKNIGSILNTGLSRCV
DITDESITNKDESMHNAKPELIQEQLKKTNHETSFPKEGSIGTNVKFRNTNNEISLKT
GDTSLPIKTLESINNHHSNDYSTNKVEKFEKENHHPPPIEDIVDMSDQTDMESNCQDG
NNLKELKVTDKNVPTDNGTNVSPRLEQNIEASGSPVQTVNKSAFLNKEFSSLNMKRKR
KRHDKNNSLTSYELERDKKRSKKNRVKLIPDNMETVSAPKIRAIYYNEAISKNPDLKE
KHEYKQAYHKELQNLKDMKVFDVDVKYSRSEIPDNLIVPTNTIFTKKRNGIYKARIVC
RGDTQSPDTYSVITTESLNHNHIKIFLMIANNRNMFMKTLDINHAFLYAKLEEEIYIP
HPHDRRCVVKLNKALYGLKQSPKEWNDHLRQYLNGIGLKDNSYTPGLYQTEDKNLMIA
VYVDDCVIAASNEQRLDEFINKLKSNFELKITGTLIDDVLDTDILGMDLVYNKRLGTI
DLTLKSFINRMDKKYNEELKKIRKSSIPHMSTYKIDPKKDVLQMSEEEFRQGVLKLQQ
LLGELNYVRHKCRYDIEFAVKKVARLVNYPHERVFYMIYKIIQYLVRYKDIGIHYDRD
CNKDKKVIAITDASVGSEYDAQSRIGVILWYGMNIFNVYSNKSTNRCVSSTEAELHAI
YEGYADSETLKVTLKELGEGDNNDIVMITDSKPAIQGLNRSYQQPKEKFTWIKTEIIK
EKIKEKSIKLLKITGKGNIADLLTKPVSASDFKRFIQVLKNKITSQDILASTDY"
repeat_region 203398..203768
/note="Ty4 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007037"
repeat_region complement(203783..204114)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007022"
rep_origin 204029..204917
/note="ARS1008; Autonomously Replicating Sequence"
/db_xref="SGD:S000028445"
gene 204735..204806
/locus_tag="YNCJ0006W"
/db_xref="GeneID:853331"
tRNA 204735..204806
/locus_tag="YNCJ0006W"
/product="tRNA-Asp"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:853331"
/db_xref="SGD:S000006536"
gene <205305..>207449
/gene="MDV1"
/locus_tag="YJL112W"
/gene_synonym="GAG3; NET2"
/db_xref="GeneID:853332"
mRNA <205305..>207449
/gene="MDV1"
/locus_tag="YJL112W"
/gene_synonym="GAG3; NET2"
/product="Mdv1p"
/transcript_id="NM_001181545.2"
/db_xref="GeneID:853332"
CDS 205305..207449
/gene="MDV1"
/locus_tag="YJL112W"
/gene_synonym="GAG3; NET2"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane
[PMID:11038180|PMID:16407407|PMID:11038182]"
/experiment="EXISTENCE:direct assay:GO:0043130 ubiquitin
binding [PMID:21070969]"
/experiment="EXISTENCE:genetic interaction:GO:0000266
mitochondrial fission [PMID:11179417]"
/experiment="EXISTENCE:genetic interaction:GO:0016559
peroxisome fission [PMID:18445678]"
/experiment="EXISTENCE:mutant phenotype:GO:0000266
mitochondrial fission
[PMID:11038183|PMID:11038180|PMID:11038182|PMID:11179417]"
/experiment="EXISTENCE:physical interaction:GO:0000266
mitochondrial fission [PMID:11038182|PMID:11179417]"
/note="Peripheral protein of cytosolic face of
mitochondrial outer membrane; required for mitochondrial
fission; interacts with Fis1p and with the self-assembled
oligomeric form of the dynamin-related GTPase Dnm1p;
contains WD repeats; MDV1 has a paralog, CAF4, that arose
from the whole genome duplication"
/codon_start=1
/product="Mdv1p"
/protein_id="NP_012423.1"
/db_xref="GeneID:853332"
/db_xref="SGD:S000003648"
/translation="MSVNDQITHIGKTLSTTASAFLNYQKSNSNTQDVLTNNGPYKNL
LSNTVNNASSTSYFYKRTEHGRFVKNASNTFEDIYSKTRRGDVFRNKFTDNKTCFRML
TYISDDLLNEIPTKEGLKSDADGKLLTEGGENENLRKNASKKETSLFQGFKSYLPIAE
LAIENTERLNYDTNGTSGTVGAKDVMSKTNERDEIHTELPNFQDSFLIPPGVETKKIS
SSYSPSALKSFSQTLVNSLEFLNIQKNSTLSEIRDIEVEVENLRQKKEKLLGKIANIE
QNQLLLEDNLKQIDDRLDFLEEYGLEVIEANSDENAEDDGMSERKALKNDAIRNEGVT
TESISSEASNLPPRRRQQLRDDNSLNRLGAFYSKSKKRHRKSFPTFQQLYEPGTKIGS
IMSTHDDFLTCLDFDAPFGTLCTAGYLDHTVKIWDLSKQNKIGELAGHLATINCMQIN
RDYGTLVTGGRDAALKLWNLNLAQQLYQETQNLTSPTNHIDSPCVHTFEAHTDEVTAL
SLDPSFLVSGSQDRTIRQWDLRSGKCLQTIDLSFANVLTTSTNVDLSKSTLLTQRNER
PSIGALQSFDAALATGTKDGVVRLWDLRSGKVIRTLKGHTDAITSLKFDSACLVTGSY
DRTVRIWDLRTGLLNKFHAYSAPVLSLDLFQENAAVVVADEPSVQIYDSEKDESWSCV
EQGNETSVSTVKYKENYMVEGRENGDVNIWAV"
gene <207877..>209529
/gene="CCT7"
/locus_tag="YJL111W"
/gene_synonym="TCP7"
/db_xref="GeneID:853333"
mRNA <207877..>209529
/gene="CCT7"
/locus_tag="YJL111W"
/gene_synonym="TCP7"
/product="chaperonin-containing T-complex subunit CCT7"
/transcript_id="NM_001181544.1"
/db_xref="GeneID:853333"
CDS 207877..209529
/gene="CCT7"
/locus_tag="YJL111W"
/gene_synonym="TCP7"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005832
chaperonin-containing T-complex [PMID:16762366]"
/experiment="EXISTENCE:direct assay:GO:0006457 protein
folding [PMID:16762366]"
/experiment="EXISTENCE:direct assay:GO:0051082 unfolded
protein binding [PMID:16762366]"
/experiment="EXISTENCE:physical interaction:GO:0005832
chaperonin-containing T-complex [PMID:15704212]"
/note="Subunit of the cytosolic chaperonin Cct ring
complex; related to Tcp1p, required for the assembly of
actin and tubulins in vivo; mutant has increased
aneuploidy tolerance"
/codon_start=1
/product="chaperonin-containing T-complex subunit CCT7"
/protein_id="NP_012424.1"
/db_xref="GeneID:853333"
/db_xref="SGD:S000003647"
/translation="MNFGSQTPTIVVLKEGTDASQGKGQIISNINACVAVQEALKPTL
GPLGSDILIVTSNQKTTISNDGATILKLLDVVHPAAKTLVDISRAQDAEVGDGTTSVT
ILAGELMKEAKPFLEEGISSHLIMKGYRKAVSLAVEKINELAVDITSEKSSGRELLER
CARTAMSSKLIHNNADFFVKMCVDAVLSLDRNDLDDKLIGIKKIPGGAMEESLFINGV
AFKKTFSYAGFEQQPKKFNNPKILSLNVELELKAEKDNAEVRVEHVEDYQAIVDAEWQ
LIFEKLRQVEETGANIVLSKLPIGDLATQFFADRNIFCAGRVSADDMNRVIQAVGGSI
QSTTSDIKPEHLGTCALFEEMQIGSERYNLFQGCPQAKTCTLLLRGGAEQVIAEVERS
LHDAIMIVKRALQNKLIVAGGGATEMEVSKCLRDYSKTIAGKQQMIINAFAKALEVIP
RQLCENAGFDAIEILNKLRLAHSKGEKWYGVVFETENIGDNFAKFVWEPALVKINALN
SATEATNLILSVDETITNKGSESANAGMMPPQGAGRGRGMPM"
gene complement(<209922..>211577)
/gene="GZF3"
/locus_tag="YJL110C"
/gene_synonym="DEH1; NIL2"
/db_xref="GeneID:853334"
mRNA complement(<209922..>211577)
/gene="GZF3"
/locus_tag="YJL110C"
/gene_synonym="DEH1; NIL2"
/product="Gzf3p"
/transcript_id="NM_001181543.1"
/db_xref="GeneID:853334"
CDS complement(209922..211577)
/gene="GZF3"
/locus_tag="YJL110C"
/gene_synonym="DEH1; NIL2"
/experiment="EXISTENCE:direct assay:GO:0000981 DNA-binding
transcription factor activity, RNA polymerase II-specific
[PMID:19380492]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:19380492]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding
[PMID:19111667|PMID:9171383]"
/experiment="EXISTENCE:genetic interaction:GO:0090295
nitrogen catabolite repression of transcription
[PMID:9106207]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:9171383]"
/experiment="EXISTENCE:mutant phenotype:GO:0000981
DNA-binding transcription factor activity, RNA polymerase
II-specific [PMID:19380492]"
/experiment="EXISTENCE:mutant phenotype:GO:0090295
nitrogen catabolite repression of transcription
[PMID:9171427|PMID:9171383]"
/experiment="EXISTENCE:physical interaction:GO:0051457
maintenance of protein location in nucleus
[PMID:19380492]"
/note="GATA zinc finger protein; negatively regulates
nitrogen catabolic gene expression by competing with Gat1p
for GATA site binding; function requires a repressive
carbon source; dimerizes with Dal80p and binds to Tor1p;
GZF3 has a paralog, DAL80, that arose from the whole
genome duplication"
/codon_start=1
/product="Gzf3p"
/protein_id="NP_012425.1"
/db_xref="GeneID:853334"
/db_xref="SGD:S000003646"
/translation="MASQATTLRGYNIRKRDNVFEPKSSENLNSLNQSEEEGHIGRWP
PLGYEAVSAEQKSAVQLRESQAGASISNNMNFKANDKSFSTSTAGRMSPDTNSLHHIL
PKNQVKNNGQTMDANCNNNVSNDANVPVCKNCLTSTTPLWRRDEHGAMLCNACGLFLK
LHGKPRPISLKTDVIKSRNRKSNTNHAHNLDNFRNQTLIAELKGDCNIESSGRKANRV
TSEDKKKKSSQLLMGTSSTAKISKKPKTESKERSDSHLSATKLEVLMSGDCSRPNLKP
KLPKQDTAIYQEKLLTFPSYTDVKEYSNSAHQSAFIKERSQFNAASFPLNASHSVTSK
TGADSPQLPHLSMLLGSLSSTSISNNGSEIVSNCNNGIASTAATLAPTSSRTTDSNPS
EVPNQIRSTMSSPDIISAKRNDPAPLSFHMASINDMLETRDRAISNVKTETTPPHFIP
FLQSSKAPCISKANSQSISNSVSSSDVSGRKFENHPAKDLGDQLSTKLHKEEEIIKLK
TRINELELVTDLYRRHINELDGKCRALEERLQRTVKQEGNKGG"
gene complement(<212000..>217309)
/gene="UTP10"
/locus_tag="YJL109C"
/db_xref="GeneID:853335"
mRNA complement(<212000..>217309)
/gene="UTP10"
/locus_tag="YJL109C"
/product="snoRNA-binding rRNA-processing protein UTP10"
/transcript_id="NM_001181542.1"
/db_xref="GeneID:853335"
CDS complement(212000..217309)
/gene="UTP10"
/locus_tag="YJL109C"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:22842922|PMID:12150911|PMID:12068309|PMID:17652137]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:17652137|PMID:12150911]"
/experiment="EXISTENCE:direct assay:GO:0030688
preribosome, small subunit precursor [PMID:17652137]"
/experiment="EXISTENCE:direct assay:GO:0032040
small-subunit processome [PMID:12068309]"
/experiment="EXISTENCE:direct assay:GO:0033553 rDNA
heterochromatin [PMID:15489292]"
/experiment="EXISTENCE:direct assay:GO:0034455 t-UTP
complex [PMID:17515605]"
/experiment="EXISTENCE:direct assay:GO:0034511 U3 snoRNA
binding [PMID:12068309]"
/experiment="EXISTENCE:mutant phenotype:GO:0000447
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from
5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:17652137]"
/experiment="EXISTENCE:mutant phenotype:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12068309]"
/experiment="EXISTENCE:mutant phenotype:GO:0000472
endonucleolytic cleavage to generate mature 5'-end of
SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:17652137]"
/experiment="EXISTENCE:mutant phenotype:GO:0000480
endonucleolytic cleavage in 5'-ETS of tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:17652137]"
/experiment="EXISTENCE:mutant phenotype:GO:0045943
positive regulation of transcription by RNA polymerase I
[PMID:15489292]"
/note="Nucleolar protein; component of the small subunit
(SSU) processome containing the U3 snoRNA that is involved
in processing of pre-18S rRNA; mutant has increased
aneuploidy tolerance"
/codon_start=1
/product="snoRNA-binding rRNA-processing protein UTP10"
/protein_id="NP_012426.1"
/db_xref="GeneID:853335"
/db_xref="SGD:S000003645"
/translation="MSSLSDQLAQVASNNATVALDRKRRQKLHSASLIYNSKTAATQD
YDFIFENASKALEELSQIEPKFAIFSRTLFSESSISLDRNVQTKEEIKDLDNAINAYL
LLASSKWYLAPTLHATEWLVRRFQIHVKNTEMLLLSTLNYYQTPVFKRILSIIKLPPL
FNCLSNFVRSEKPPTALTMIKLFNDMDFLKLYTSYLDQCIKHNATYTNQLLFTTCCFI
NVVAFNSNNDEKLNQLVPILLEISAKLLASKSKDCQIAAHTILVVFATALPLKKTIIL
AAMETILSNLDAKEAKHSALLTICKLFQTLKGQGNVDQLPSKIFKLFDSKFDTVSILT
FLDKEDKPVCDKFITSYTRSIARYDRSKLNIILSLLKKIRLERYEVRLIITDLIYLSE
ILEDKSQLVELFEYFISINEDLVLKCLKSLGLTGELFEIRLTTSLFTNADVNTDIVKQ
LSDPVETTKKDTASFQTFLDKHSELINTTNVSMLTETGERYKKVLSLFTEAIGKGYKA
SSFLTSFFTTLESRITFLLRVTISPAAPTALKLISLNNIAKYINSIEKEVNIFTLVPC
LICALRDASIKVRTGVKKILSLIAKRPSTKHYFLSDKLYGENVTIPMLNPKDSEAWLS
GFLNEYVTENYDISRILTPKRNEKVFLMFWANQALLIPSPYAKTVLLDNLNKSPTYAS
SYSSLFEEFISHYLENRSSWEKSCIANKTNFEHFERSLVNLVSPKEKQSFMIDFVLSA
LNSDYEQLANIAAERLISIFASLNNAQKLKIVQNIVDSSSNVESSYDTVGVLQSLPLD
SDIFVSILNQNSISNEMDQTDFSKRRRRRSSTSKNAFLKEEVSQLAELHLRKLTIILE
ALDKVRNVGSEKLLFTLLSLLSDLETLDQDGGLPVLYAQETLISCTLNTITYLKEHGC
TELTNVRADILVSAIRNSASPQVQNKLLLVIGSLATLSSEVILHSVMPIFTFMGAHSI
RQDDEFTTKVVERTILTVVPALIKNSKGNEKEEMEFLLLSFTTALQHVPRHRRVKLFS
TLIKTLDPVKALGSFLFLIAQQYSSALVNFKIGEARILIEFIKALLVDLHVNEELSGL
NDLLDIIKLLTSSKSSSEKKKSLESRVLFSNGVLNFSESEFLTFMNNTFEFINKITEE
TDQDYYDVRRNLRLKVYSVLLDETSDKKLIRNIREEFGTLLEGVLFFINSVELTFSCI
TSQENEEASDSETSLSDHTTEIKEILFKVLGNVLQILPVDEFVNAVLPLLSTSTNEDI
RYHLTLVIGSKFELEGSEAIPIVNNVMKVLLDRMPLESKSVVISQVILNTMTALVSKY
GKKLEGSILTQALTLATEKVSSDMTEVKISSLALITNCVQVLGVKSIAFYPKIVPPSI
KLFDASLADSSNPLKEQLQVAILLLFAGLIKRIPSFLMSNILDVLHVIYFSREVDSSI
RLSVISLIIENIDLKEVLKVLFRIWSTEIATSNDTVAVSLFLSTLESTVENIDKKSAT
SQSPIFFKLLLSLFEFRSISSFDNNTISRIEASVHEISNSYVLKMNDKVFRPLFVILV
RWAFDGEGVTNAGITETERLLAFFKFFNKLQENLRGIITSYFTYLLEPVDMLLKRFIS
KDMENVNLRRLVINSLTSSLKFDRDEYWKSTSRFELISVSLVNQLSNIENSIGKYLVK
AIGALASNNSGVDEHNQILNKLIVEHMKASCSSNEKLWAIRAMKLIYSKIGESWLVLL
PQLVPVIAELLEDDDEEIEREVRTGLVKVVENVLGEPFDRYLD"
gene complement(<217705..>218856)
/gene="PRM10"
/locus_tag="YJL108C"
/db_xref="GeneID:853336"
mRNA complement(<217705..>218856)
/gene="PRM10"
/locus_tag="YJL108C"
/product="pheromone-regulated protein PRM10"
/transcript_id="NM_001181541.1"
/db_xref="GeneID:853336"
CDS complement(217705..218856)
/gene="PRM10"
/locus_tag="YJL108C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/note="Pheromone-regulated protein; proposed to be
involved in mating; predicted to have 5 transmembrane
segments; induced by treatment with 8-methoxypsoralen and
UVA irradiation"
/codon_start=1
/product="pheromone-regulated protein PRM10"
/protein_id="NP_012427.1"
/db_xref="GeneID:853336"
/db_xref="SGD:S000003644"
/translation="MIVSFGDATTRTSEVQLVRCTQGLNLWKLHQVHAVYKRVVHDTL
GADEGNALLDQILADTNLYPPWMCVLLYAFCSAMVTPYAFGGDWVNLAISFFMGLCVG
SLQFILSQKSYMYSNVFEISASIVVSFCGRAFGSIPRSHICFGAVTQGSLALILPGYI
ILCGALELQSRSLVAGAVRMFYAIIYSLFLGFGITLGSALFGWMYHNATNEISCPQLI
SPWFRFLFVPAFTISISLLNQAHISQLPVMVFISCTGYVVTYWAGKHFANSTEFTAAL
AAFVIGVLGNLYSRIWKGLAVSAMLPAIFVQVPSGIASQNSLLSGLQSANTIVNANET
ITTSTSDPSSSMSFGMTMIQVCVGISVGLFASSLFVYPFGKKKTGLFSL"
gene complement(<218853..>220016)
/locus_tag="YJL107C"
/db_xref="GeneID:853337"
mRNA complement(<218853..>220016)
/locus_tag="YJL107C"
/product="uncharacterized protein"
/transcript_id="NM_001181540.1"
/db_xref="GeneID:853337"
CDS complement(218853..220016)
/locus_tag="YJL107C"
/note="hypothetical protein; expression is induced by
activation of the HOG1 mitogen-activated signaling pathway
and this induction is Hog1p/Pbs2p dependent; YJL107C and
adjacent ORF, YJL108C are merged in related fungi"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_012428.1"
/db_xref="GeneID:853337"
/db_xref="SGD:S000003643"
/translation="MDGRNEKPTTPVSDFRVGSSEQSQAGVNLEDSSDHRTSNSAESK
KGNLSGKSISDLGISNNDNKNVRFTADTDALENDLSSRSTETSDNSKGTDGQDEEDRP
ARHKRKPKVSFTHLRNNGKDGDDETFIKKIINNLTGNQGGLVPGLAPIPSENENGKND
IEKNNRNEEIPLSDLADASKIVDVHEGDDKEKLEALKLEGDVNCTSDGETLGSSSKNS
FLAPAVDHFDDYAENNSSDDNEGFIETSTYVPPPSQVKSGVLGSLLKLYQNEDQNSSS
IFSDSQAVTTDDEGISSTAGNKDVPVAKRSRLQNLKGKAKKGRMPRLKKRLKTEAKIT
VHIADILQRHRFILRMCRALMMYGAPTHRLEEYMVMTSRVLEIDGQFCIFQVV"
gene <221390..>223327
/gene="IME2"
/locus_tag="YJL106W"
/gene_synonym="SME1"
/db_xref="GeneID:853338"
mRNA <221390..>223327
/gene="IME2"
/locus_tag="YJL106W"
/gene_synonym="SME1"
/product="protein kinase IME2"
/transcript_id="NM_001181539.1"
/db_xref="GeneID:853338"
CDS 221390..223327
/gene="IME2"
/locus_tag="YJL106W"
/gene_synonym="SME1"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894|PMID:7764269]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:7764269]"
/experiment="EXISTENCE:mutant phenotype:GO:0040020
regulation of meiotic nuclear division [PMID:9003314]"
/note="Serine/threonine protein kinase involved in
activation of meiosis; associates with Ime1p and mediates
its stability, activates Ndt80p; IME2 expression is
positively regulated by Ime1p; human CDK2 can complement
ime2 null mutant"
/codon_start=1
/product="protein kinase IME2"
/protein_id="NP_012429.1"
/db_xref="GeneID:853338"
/db_xref="SGD:S000003642"
/translation="MVEKRSRQSSSSGSEFSVPPDVDNPPLSIPLKTLSDRYQLIEKL
GAGSFGCVTLAKAQFPLSNILGKQHDIRGTLMDQPKNGHQNYITKTQGVVAIKTMMTK
LHTLQDYTRVREIKFILAIPANDHLIQIFEVFIDSENYQLHIVMECMEQNLYQMMKHR
RRRVFSIPSLKSILSQILAGLKHIHEHNFFHRDLKPENILITPSTQYFEKEYMNQIGY
QDNYVIKLADFGLARHVENKNPYTAYVSTRWYRSPEILLRSGYYSKPLDIWAFGCVAV
EVTVFRALFPGANEIDQIWKILEVLGTPIKRSDFVNTNHITAPPPGGFWDDASNLVHK
LNLKLPYVEGSSLDHLLSSSQLSDLSEVVKKCLRWDPNERATAQELCEMPFFENTVAS
QVDARGNVTNTEQALIFAGINPVATNTKPIYFNSSTKLPAETESNDIDISNNDHDSHA
MCSPTLNQEKLTLVEFLNEFVEEDNDDHSIPDVGTDSTISDSIDETELSKEIRNNLAL
CQLPDEEVLDHSLSNIRQLTNDIEIINKDEADNMEQLFFDLEIPEKDEFQRKQPFNEH
ADIDEDIVLPYVNNSNYTHTDRSHHRGDNVLGDASLGDSFNSMPDFTPRNFLIPTLKK
SREKFEPHLSNSNQHFGNVTF"
gene <225055..>226737
/gene="SET4"
/locus_tag="YJL105W"
/db_xref="GeneID:853339"
mRNA <225055..>226737
/gene="SET4"
/locus_tag="YJL105W"
/product="Set4p"
/transcript_id="NM_001181538.1"
/db_xref="GeneID:853339"
CDS 225055..226737
/gene="SET4"
/locus_tag="YJL105W"
/experiment="EXISTENCE:direct assay:GO:0006338 chromatin
remodeling [PMID:34625508]"
/experiment="EXISTENCE:mutant phenotype:GO:1900407
regulation of cellular response to oxidative stress
[PMID:30082318]"
/note="Chromatin-associated protein involved in control of
stress response; regulates response to oxidative stress;
involved in control of gene repression in subtelomeric
chromatin; contains a SET domain; SET4 has a paralog,
SET3, that arose from the whole genome duplication"
/codon_start=1
/product="Set4p"
/protein_id="NP_012430.1"
/db_xref="GeneID:853339"
/db_xref="SGD:S000003641"
/translation="MTSPESLSSRHIRQGRTYTTTDKVISRSSSYSSNSSMSKDYGDH
TPLSVSSAASETLPSPQYMPIRTFNTMPTAGPTPLHLFQNDRGIFNHHSSSGSSKTAS
TNKRGIAAAVALATAATIPFPLKKQNQDDNSKVSVTHNESSKENKITPSMRAEDNKPK
NGCICGSSDSKDELFIQCNKCKTWQHKLCYAFKKSDPIKRDFVCKRCDSDTKVQVNQV
KPMIFPRKMGDERLFQFSSIVTTSASNTNQHQQSVNNIEEQPKKRQLHYTAPTTENSN
SIRKKLRQEKLVVSSHFLKPLLNEVSSSNDTEFKAITISEYKDKYVKMFIDNHYDDDW
VVCSNWESSRSADIEVRKSSNERDFGVFAADSCVKGELIQEYLGKIDFQKNYQTDPNN
DYRLMGTTKPKVLFHPHWPLYIDSRETGGLTRYIRRSCEPNVELVTVRPLDEKPRGDN
DCRVKFVLRAIRDIRKGEEISVEWQWDLRNPIWEIINASKDLDSLPDPDKFWLMGSIK
TILTNCDCACGYLGHNCPITKIKNFSEEFMRNTKESLSNKSYFNTIMHNCKP"
gene <227327..>227776
/gene="PAM16"
/locus_tag="YJL104W"
/gene_synonym="MIA1; TIM16"
/db_xref="GeneID:853340"
mRNA <227327..>227776
/gene="PAM16"
/locus_tag="YJL104W"
/gene_synonym="MIA1; TIM16"
/product="import motor complex subunit PAM16"
/transcript_id="NM_001181537.1"
/db_xref="GeneID:853340"
CDS 227327..227776
/gene="PAM16"
/locus_tag="YJL104W"
/gene_synonym="MIA1; TIM16"
/experiment="EXISTENCE:direct assay:GO:0001405 PAM
complex, Tim23 associated import motor
[PMID:14981506|PMID:14981507]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:24769239|PMID:16823961|PMID:14576278|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005744 TIM23
mitochondrial import inner membrane translocase complex
[PMID:14981506]"
/experiment="EXISTENCE:direct assay:GO:0019904 protein
domain specific binding [PMID:16105940]"
/experiment="EXISTENCE:mutant phenotype:GO:0030150 protein
import into mitochondrial matrix
[PMID:14981507|PMID:14981506]"
/note="Subunit of the import motor (PAM complex); the PAM
complex is a component of the Translocase of the Inner
Mitochondrial membrane (TIM23 complex); forms a 1:1
subcomplex with Pam18p and inhibits its cochaperone
activity; contains a J-like domain"
/codon_start=1
/product="import motor complex subunit PAM16"
/protein_id="NP_012431.1"
/db_xref="GeneID:853340"
/db_xref="SGD:S000003640"
/translation="MAHRAFIQVIITGTQVFGKAFAEAYRQAASQSVKQGATNASRRG
TGKGEYGGITLDESCKILNIEESKGDLNMDKINNRFNYLFEVNDKEKGGSFYLQSKVY
RAAERLKWELAQREKNAKAKAGDASTAKPPPNSTNSSGADNSASSNQ"
gene complement(228094..228479)
/gene="SNR37"
/locus_tag="YNCJ0007C"
/db_xref="GeneID:9164908"
ncRNA complement(228094..228479)
/ncRNA_class="snoRNA"
/gene="SNR37"
/locus_tag="YNCJ0007C"
/product="SNR37"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:9160748]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:35385737]"
/experiment="EXISTENCE:mutant phenotype:GO:0030559 rRNA
pseudouridylation guide activity
[PMID:35385737|PMID:9160748]"
/experiment="EXISTENCE:mutant phenotype:GO:0031118 rRNA
pseudouridine synthesis [PMID:9160748]"
/experiment="EXISTENCE:mutant phenotype:GO:0042254
ribosome biogenesis [PMID:35385737]"
/experiment="EXISTENCE:physical interaction:GO:0031429 box
H/ACA snoRNP complex [PMID:9472021|PMID:15923376]"
/note="H/ACA box small nucleolar RNA (snoRNA) involved in
ribosome synthesis; has structural role in early pre-60S
pathway; guides pseudouridylation of large subunit (LSU)
rRNA at position U2944"
/transcript_id="NR_132195.1"
/db_xref="GeneID:9164908"
/db_xref="SGD:S000006501"
rep_origin 228552..229043
/note="ARS1009; Autonomously Replicating Sequence"
/db_xref="SGD:S000028446"
gene complement(<229025..>230881)
/gene="GSM1"
/locus_tag="YJL103C"
/db_xref="GeneID:853341"
mRNA complement(<229025..>230881)
/gene="GSM1"
/locus_tag="YJL103C"
/product="Gsm1p"
/transcript_id="NM_001181536.1"
/db_xref="GeneID:853341"
CDS complement(229025..230881)
/gene="GSM1"
/locus_tag="YJL103C"
/experiment="EXISTENCE:curator inference:GO:0005634
nucleus [PMID:16785442]"
/experiment="EXISTENCE:direct assay:GO:0003700 DNA-binding
transcription factor activity [PMID:16785442]"
/experiment="EXISTENCE:direct assay:GO:0006357 regulation
of transcription by RNA polymerase II [PMID:16785442]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:19158363]"
/experiment="EXISTENCE:mutant phenotype:GO:0003700
DNA-binding transcription factor activity [PMID:16785442]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:16785442]"
/note="Putative zinc cluster hypothetical protein;
proposed to be involved in the regulation of energy
metabolism, based on patterns of expression and sequence
analysis"
/codon_start=1
/product="Gsm1p"
/protein_id="NP_012432.1"
/db_xref="GeneID:853341"
/db_xref="SGD:S000003639"
/translation="MTKKLPSELKQTRKSIQTACEFCHTKHIQCDVGRPCQNCLKRNI
GKFCRDKKRKSRKRIEKHGTQPYLNLGKRLVIHDVPSKTVSPSSVHLQRDFLSSDQEK
PGKTPAHNTNIQYTYNINDNFQSAGSIPRITNFNTNNRQTVLENTSNNISASQAVHLM
NDPIIPTVRKSTLNLKSHFLEQHKAMQQPLATNCLVATSNVPVHSGMDDSNKSDDDVD
DETNIHFDSMWCNDEYMKLKDIVDISTPFLPNNSQIFSLQESEYPNPSASTRGNSSLH
LTNLLNSTKSVNDQKDSSIGHSTSTFNTYDEVVSRPFISLDMLHLNRGANANTHPSHN
AKLESECDSSSHSDADLEKHDTDFISPSKFRELVKTPQDLYDNKCLIKPHNYKLAYTK
LLTTLRKKFLEGAEIDKSASVKDEHSTQKHNLRYDLEVIIRSILERYAPIFISLTSNM
IEEDLLLQEVTLQRALLDLENMAKLVSCTPMCIWRRSGEICFVSNEFYSLTGFNKNLL
LDRTSFIFEYLDHKSVSNYFQIFNELLAFGYNDINKRKKLLMLNACSSTSSKITEGFS
FTTDGKAIFTKCNLLLSNGLYLKCACCWTVKRDSFNIPILVMGQFLPIFEMD"
gene <231301..>233760
/gene="MEF2"
/locus_tag="YJL102W"
/db_xref="GeneID:853342"
mRNA <231301..>233760
/gene="MEF2"
/locus_tag="YJL102W"
/product="mitochondrial elongation factor MEF2"
/transcript_id="NM_001181535.1"
/db_xref="GeneID:853342"
CDS 231301..233760
/gene="MEF2"
/locus_tag="YJL102W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0051881
regulation of mitochondrial membrane potential
[PMID:21414316]"
/note="Mitochondrial elongation factor involved in
translational elongation"
/codon_start=1
/product="mitochondrial elongation factor MEF2"
/protein_id="NP_012433.1"
/db_xref="GeneID:853342"
/db_xref="SGD:S000003638"
/translation="MWKWNVRRWAGARVNISKNRLSVINVGSRYLSTARSPLSKVRNI
GIIAHIDAGKTTTTERMLYYAGISKHIGDVDTGDTITDFLEQERSRGITIQSAAISFP
WRNTFAINLIDTPGHIDFTFEVIRALKVIDSCVVILDAVAGVEAQTEKVWKQSKSKPK
ICFINKMDRMGASFNHTVNDLINKFMRGTTTKPVLVNIPYYRKQPTSNDYVFQGVIDV
VNGKRLTWNPENPDEIIVDELDGTSLEQCNRCRESMIETLTEYDEDLVQHFLEEAEGD
YSKVSAQFLNASIRKLTMKNMIVPVLCGASFKNIGVQPLLDAIVNYLPSPIEAELPEL
NDKTVPMKYDPKVGCLVNNNKNLCIALAFKVITDPIRGKQIFIRIYSGTLNSGNTVYN
STTGEKFKLGKLLIPHAGTSQPVNILTAGQIGLLTGSTVENNISTGDTLITHSSKKDG
LKSLDKKKELTLKINSIFIPPPVFGVSIEPRTLSNKKSMEEALNTLITEDPSLSISQN
DETGQTVLNGMGELHLEIAKDRLVNDLKADVEFGQLMVSYKETINSETNIETYESDDG
YRFSLSLLPNSDALPNCLAYPLGVNENFLIMEKNGNWDKEWKYQVSFESILNSIIASC
IVGLQRGGKIANFPLYACSIKINSDWSVPPDIETPQEILKITRNLIFKALNDLKPEKY
NLLEPIMNLDLTIPQSDVGTVLQDLTGARKAQILSIEDESSVSNSGASTCNSPENSNR
IYIPSDAVTTLHATKDKKNTQETSSNVKKIIKAKVPLREITTYTNKLRSLSQGRGEFN
IEYSDMEKVTNDRLQSILHDL"
gene 233939..234011
/locus_tag="YNCJ0008W"
/db_xref="GeneID:853343"
tRNA 233939..234011
/locus_tag="YNCJ0008W"
/product="tRNA-Arg"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE
analysis; one of 6 nuclear tRNA genes containing the
tDNA-anticodon ACG (converted to ICG in the mature tRNA),
decodes CGU, CGC, and probably CGA codons into arginine,
one of 19 nuclear tRNAs for arginine"
/db_xref="GeneID:853343"
/db_xref="SGD:S000006702"
gene complement(<234320..>236356)
/gene="GSH1"
/locus_tag="YJL101C"
/db_xref="GeneID:853344"
mRNA complement(<234320..>236356)
/gene="GSH1"
/locus_tag="YJL101C"
/product="glutamate--cysteine ligase"
/transcript_id="NM_001181534.1"
/db_xref="GeneID:853344"
CDS complement(234320..236356)
/gene="GSH1"
/locus_tag="YJL101C"
/EC_number="6.3.2.2"
/experiment="EXISTENCE:direct assay:GO:0004357
glutamate-cysteine ligase activity [PMID:1687097]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:2184310]"
/experiment="EXISTENCE:direct assay:GO:0006750 glutathione
biosynthetic process [PMID:1687097]"
/experiment="EXISTENCE:direct assay:GO:0046686 response to
cadmium ion [PMID:10921921]"
/experiment="EXISTENCE:mutant phenotype:GO:0004357
glutamate-cysteine ligase activity [PMID:2184310]"
/experiment="EXISTENCE:mutant phenotype:GO:0006750
glutathione biosynthetic process [PMID:8768524]"
/experiment="EXISTENCE:mutant phenotype:GO:0042542
response to hydrogen peroxide [PMID:7615092]"
/note="Gamma glutamylcysteine synthetase; catalyzes the
first step in glutathione (GSH) biosynthesis; expression
induced by oxidants, cadmium, and mercury; protein
abundance increases in response to DNA replication stress"
/codon_start=1
/product="glutamate--cysteine ligase"
/protein_id="NP_012434.1"
/db_xref="GeneID:853344"
/db_xref="SGD:S000003637"
/translation="MGLLALGTPLQWFESRTYNEHIRDEGIEQLLYIFQAAGKRDNDP
LFWGDELEYMVVDFDDKERNSMLDVCHDKILTELNMEDSSLCEANDVSFHPEYGRYML
EATPASPYLNYVGSYVEVNMQKRRAIAEYKLSEYARQDSKNNLHVGSRSVPLTLTVFP
RMGCPDFINIKDPWNHKNAASRSLFLPDEVINRHVRFPNLTASIRTRRGEKVCMNVPM
YKDIATPETDDSIYDRDWFLPEDKEAKLASKPGFIYMDSMGFGMGCSCLQVTFQAPNI
NKARYLYDALVNFAPIMLAFSAAAPAFKGWLADQDVRWNVISGAVDDRTPKERGVAPL
LPKYNKNGFGGIAKDVQDKVLEIPKSRYSSVDLFLGGSKFFNRTYNDTNVPINEKVLG
RLLENDKAPLDYDLAKHFAHLYIRDPVSTFEELLNQDNKTSSNHFENIQSTNWQTLRF
KPPTQQATPDKKDSPGWRVEFRPFEVQLLDFENAAYSVLIYLIVDSILTFSDNINAYI
HMSKVWENMKIAHHRDAILFEKFHWKKSFRNDTDVETEDYSISEIFHNPENGIFPQFV
TPILCQKGFVTKDWKELKHSSKHERLYYYLKLISDRASGELPTTAKFFRNFVLQHPDY
KHDSKISKSINYDLLSTCDRLTHLDDSKGELTSFLGAEIAEYVKKNKPSIESKC"
gene <237263..>239086
/gene="LSB6"
/locus_tag="YJL100W"
/db_xref="GeneID:853345"
mRNA <237263..>239086
/gene="LSB6"
/locus_tag="YJL100W"
/product="1-phosphatidylinositol 4-kinase LSB6"
/transcript_id="NM_001181533.1"
/db_xref="GeneID:853345"
CDS 237263..239086
/gene="LSB6"
/locus_tag="YJL100W"
/EC_number="2.7.1.67"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane
[PMID:12523934|PMID:26928762|PMID:12361950]"
/experiment="EXISTENCE:direct assay:GO:0004430
1-phosphatidylinositol 4-kinase activity
[PMID:12361950|PMID:12523934]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:12361950|PMID:12523934]"
/experiment="EXISTENCE:direct assay:GO:0046854
phosphatidylinositol phosphate biosynthetic process
[PMID:12361950|PMID:12523934]"
/experiment="EXISTENCE:mutant phenotype:GO:0004430
1-phosphatidylinositol 4-kinase activity [PMID:12361950]"
/experiment="EXISTENCE:mutant phenotype:GO:0046854
phosphatidylinositol phosphate biosynthetic process
[PMID:12361950]"
/note="Type II phosphatidylinositol 4-kinase; binds
Las17p, a homolog of human Wiskott-Aldrich Syndrome
protein involved in actin patch assembly and actin
polymerization"
/codon_start=1
/product="1-phosphatidylinositol 4-kinase LSB6"
/protein_id="NP_012435.1"
/db_xref="GeneID:853345"
/db_xref="SGD:S000003636"
/translation="MSNEAYQHDHTVNPHQKIVVNSYDWLQFRDEQDHCKSKNPITHA
SPGVGSNAQNSDIAEAPQVFHPSYQSLVNVPSESPRPDQTSGSNPAVGLLHNAEDKAS
GQEEEGSQYEIQYSVFRPLHAYPTKGLAYEQLRRKEEQEQRENFNHLVSDCIEAVETF
GRELERIQTGSSGSYFVYGTRADESVPVGVFKPKDEEPYGPFSPKWTKWAHRTFFPCL
FGRSCLIPNLGYICESAASLLDRRLETHLVPYTDTASIESFNFYDNRKKWVLGYNLQK
KKQKKLGSFQLFLKEYINADEFFHKYPLPGMYSDVKHSFHRKSSGEDINHKPETTRNL
TDETEPSKQINSSPISTESEENSKFEWTESSLSQFRLELEKLIILDYIMRNTDRGLDN
WMVKLIKLSNNKWRLKLAAIDNGLSFPWKHPDEWRLYPYGWLYLPLQLLAKPFSEQMR
SHFLPILTSTNWWEESYQEFLALFSRDQDFNVRMWKKQWAVLKGQAFNVVETLKDPRQ
GPLELVRRTRCQVIDEKMQVPCCPPPVSIFKNAIDEPIGSYSTSPMVLPSTPSTIPFH
AHNQSNSNPVYYDSTLHPFANKTVIAERLQIVNSTPVFTWC"
gene <239414..>241654
/gene="CHS6"
/locus_tag="YJL099W"
/gene_synonym="CSD3"
/db_xref="GeneID:853346"
mRNA <239414..>241654
/gene="CHS6"
/locus_tag="YJL099W"
/gene_synonym="CSD3"
/product="Chs6p"
/transcript_id="NM_001181532.1"
/db_xref="GeneID:853346"
CDS 239414..241654
/gene="CHS6"
/locus_tag="YJL099W"
/gene_synonym="CSD3"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0030140 trans-Golgi
network transport vesicle [PMID:16498409]"
/experiment="EXISTENCE:direct assay:GO:0034044 exomer
complex [PMID:17000877|PMID:23000721]"
/experiment="EXISTENCE:genetic interaction:GO:0006893
Golgi to plasma membrane transport [PMID:16498409]"
/experiment="EXISTENCE:mutant phenotype:GO:0006031 chitin
biosynthetic process [PMID:9614194]"
/experiment="EXISTENCE:mutant phenotype:GO:0006893 Golgi
to plasma membrane transport [PMID:9614194|PMID:11879634]"
/note="Member of the ChAPs (Chs5p-Arf1p-binding proteins)
family; part of the exomer complex that mediates export of
specific cargo proteins, including Chs3p, from the Golgi
to the plasma membrane; primary component of the Chs5/6
complex that binds directly to membranes; CHS6 has a
paralog, BCH2, that arose from the whole genome
duplication"
/codon_start=1
/product="Chs6p"
/protein_id="NP_012436.1"
/db_xref="GeneID:853346"
/db_xref="SGD:S000003635"
/translation="MNLFWPSETKKQNEIPGGDYTPGNSPSVQKGYQFLNRDIFKSCP
RIMERQFGECLHNRTHLIKDLISSGNVGLGPIEIVHMSYLNKHEKEEFGEYFYVTGIE
VSGPAMPVEFLEVLKSSKRISKNISNNIILTYCCFNFFSNLDIRIRYDADDTFQTTAI
DCNKETTDLTMTEKMWEETFASSVIRAIITNTNPELKPPGLVECPFYVGKDTISSCKK
IIELLCRFLPRSLNCGWDSTKSMQATIVNNYLMYSLKSFIAITPSLVDFTIDYLKGLT
KKDPIHDIYYKTAMITILDHIETKELDMITILNETLDPLLSLLNDLPPRDADSARLMN
CMSDLLNIQTNFLLNRGDYELALGVSNTSTELALDSFESWYNLARCHIKKEEYEKALF
AINSMPRLRKNDGHLETMYSRFLTSNYYKKPLNGTREHYDLTAMEFTNLSGTLRNWKE
DELKRQIFGRIAMINEKKIGYTKEIWDDIAIKLGPICGPQSVNLINYVSPQEVKNIKN
INLIARNTIGKQLGWFSGKIYGLLMEIVNKIGWNGLLNIRTEAFMMETEFYQASNNII
DENGHIPMESRKKRFCEGWLDDLFLDLYQDLKLSKISLSNKDEKHSGLEWELLGLIML
RTWHWEDAVACLRTSIVARFDPVSCQQLLKIYLQPPKNIQEVTLLDTDTIISLLIKKI
SYDCRYYNYCQIFNLQLLEKLCNELGTHILRNKILLQPSIGDEIMVMIDAMLAWIADL
DHTVQP"
gene <242082..>245258
/gene="SAP185"
/locus_tag="YJL098W"
/db_xref="GeneID:853347"
mRNA <242082..>245258
/gene="SAP185"
/locus_tag="YJL098W"
/product="Sap185p"
/transcript_id="NM_001181531.1"
/db_xref="GeneID:853347"
CDS 242082..245258
/gene="SAP185"
/locus_tag="YJL098W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:1903766 positive
regulation of potassium ion export across plasma membrane
[PMID:15947196]"
/experiment="EXISTENCE:genetic interaction:GO:0002098 tRNA
wobble uridine modification [PMID:18755837]"
/experiment="EXISTENCE:mutant phenotype:GO:0000082 G1/S
transition of mitotic cell cycle [PMID:8649382]"
/experiment="EXISTENCE:mutant phenotype:GO:1903766
positive regulation of potassium ion export across plasma
membrane [PMID:15947196]"
/note="Protein that forms a complex with the Sit4p protein
phosphatase; required for Sit4p function; member of a
family of similar proteins including Sap4p, Sap155p, and
Sap190p; SAP185 has a paralog, SAP190, that arose from the
whole genome duplication"
/codon_start=1
/product="Sap185p"
/protein_id="NP_012437.1"
/db_xref="GeneID:853347"
/db_xref="SGD:S000003634"
/translation="MSGSFWKFGQDFGSQSPLAKLLNRAFIKIDDKPTSTEAGKIDSN
STDESLESNSFKSEDEEEEYELPNREEDYKAYKPNLSLLNDLLDDEELYTELMCSNFK
LLVYLKYPEVLSKLIDYVRNSTILESNIDRVTSEDRDLVRGEDKDTTEDFENAKADKK
NIDGTFEEKERTRSGEEEELENEENDSASEDTRVTLPHELEEHDDTRRARIAAEILSA
DVWPISSALIENEGLLAKLWSILRLPSPLSIEASTYFMKINERLLDMNMDGIIEFILK
KEHIVDDFLAHIDNPPLMDFLLKVISTDKPEISNGVIQLFKKQNLVPKLIHLLDPVFD
SCTQSAAGDFLKALVTISGNCPNEITSSIGPNELTRQLVSPNMMKQLMDIMLKGGNSL
NNGVGIIIELIRKNNSDYDTIQTNYTTIESHPPTDRDPIYLGYLVKMFSEHMADFNKI
LTEKKIPLLQTSYGTIEPLGFERFKICELIAELLHCSNMTLLNEPSAYDIVRERDAER
ERIFNSQNYVDSNDRSELKENEDDNTGDADDEVEDDTNQVESANTSIDGEEVIDKLNS
LQIETNKVNQNMNNEEQHSLMPDFNNGDFKDEEDENPFEPQYSDVILDSSDIEKNFRV
SPNVGDQLKISLQDTRVIDTMLEMFFHFQWNNFLHNVVYDVVQQIFNGPLKIGYNRFL
LDDLLINIRLTDMIINGNNECIEYEKGHDTRLGYMGHLTLIAEEVTKFTAYIEEMNIT
FENTEVMSSLFESKWIAYTEDVLEDLKEKYNAILGDIAEEGDMLQDEEEDAVYDKGER
TMGTVDDYINDIMQMDNVRCQEEEEDEGEGYVSFDEDEPQEYRNGDSVRSKESNSSEG
KRDQEQLYYEYVNEDGTKTRLNFNPDSDATEQVPGEVNRDHKIPLKLKRSFTDACKSE
TIPNNTVNAKEESVFQFSNELSDGWESSPSNSIPKRASPSKNGMNSPMFQHQFELHSP
TDEFGGHKDEILSAEGHDYDIDEYDELSDDSDEEYDNCEDEDSLDYADSAAYALCRSK
SKDKISWDEEEQARLMGVVKFNSEHYRD"
gene <245591..>246244
/gene="PHS1"
/locus_tag="YJL097W"
/db_xref="GeneID:853348"
mRNA <245591..>246244
/gene="PHS1"
/locus_tag="YJL097W"
/product="enoyl-CoA hydratase PHS1"
/transcript_id="NM_001181530.1"
/db_xref="GeneID:853348"
CDS 245591..246244
/gene="PHS1"
/locus_tag="YJL097W"
/EC_number="4.2.1.134"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:23708375|PMID:14562095|PMID:14690591]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:14690591]"
/experiment="EXISTENCE:direct assay:GO:0004300 enoyl-CoA
hydratase activity [PMID:18272525]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:18272525]"
/experiment="EXISTENCE:direct assay:GO:0042175 nuclear
outer membrane-endoplasmic reticulum membrane network
[PMID:14690591]"
/experiment="EXISTENCE:mutant phenotype:GO:0007034
vacuolar transport [PMID:16943325]"
/experiment="EXISTENCE:mutant phenotype:GO:0018812
3-hydroxyacyl-CoA dehydratase activity [PMID:23416297]"
/experiment="EXISTENCE:mutant phenotype:GO:0030148
sphingolipid biosynthetic process [PMID:18272525]"
/experiment="EXISTENCE:mutant phenotype:GO:0030497 fatty
acid elongation [PMID:23416297]"
/note="Essential 3-hydroxyacyl-CoA dehydratase of the ER
membrane; involved in elongation of very long-chain fatty
acids; evolutionarily conserved, similar to mammalian
PTPLA and PTPLB; involved in sphingolipid biosynthesis and
protein trafficking"
/codon_start=1
/product="enoyl-CoA hydratase PHS1"
/protein_id="NP_012438.1"
/db_xref="GeneID:853348"
/db_xref="SGD:S000003633"
/translation="MSKKLASPLSFLPLYNLLSAVGWSYLLYLVISLYPKVGQPAFFY
QTKNVATLVQCGAIIEIINSFLGVVRSPLLTTVAQVSSRLLVVLGIFQLLPNTSGVQS
VVYISLLLAWSITEIVRYLYYFFMLVFKNGAPKILILLRYNLFWILYPTGVASELRII
YCALNAAESQYSLLYKRILIAAMLAYIPGFPMLFLHMVAQRKKVMKSLRSSFGKKLI"
gene <246490..>246975
/gene="MRPL49"
/locus_tag="YJL096W"
/gene_synonym="bL21m"
/db_xref="GeneID:853349"
mRNA <246490..>246975
/gene="MRPL49"
/locus_tag="YJL096W"
/gene_synonym="bL21m"
/product="mitochondrial 54S ribosomal protein bL21m
MRPL49"
/transcript_id="NM_001181529.1"
/db_xref="GeneID:853349"
CDS 246490..246975
/gene="MRPL49"
/locus_tag="YJL096W"
/gene_synonym="bL21m"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:12392552|PMID:9151978]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:12392552|PMID:9151978]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005762
mitochondrial large ribosomal subunit
[PMID:12392552|PMID:9151978]"
/note="Mitochondrial ribosomal protein of the large
subunit"
/codon_start=1
/product="mitochondrial 54S ribosomal protein bL21m
MRPL49"
/protein_id="NP_012439.2"
/db_xref="GeneID:853349"
/db_xref="SGD:S000003632"
/translation="MLQLKFIWPVARITPIYRPFTSHPFRNLATSSSISSTKAKTTKT
DTTPLKLSNELYAIFKIHNRPYLVTEGDRVILPFKLKQAEVGDILNMTDVTTLGSRNY
KLVGHPINTSLYTLKATVVGKTKRAFQTREVTKRRNRRVRHAKSKGDLTILRISELSM
N"
misc_feature 247060..247082
/note="ETC3; Chromosome-organizing-clamp; tethers
chromosomal regions to the nuclear periphery; binds TFIIIC
transcription factor but does not recruit RNA Polymerase
III; located between MRPL49 and BCK1"
/db_xref="SGD:S000077074"
gene <247255..>251691
/gene="BCK1"
/locus_tag="YJL095W"
/gene_synonym="LAS3; SAP3; SLK1; SSP31"
/db_xref="GeneID:853350"
mRNA <247255..>251691
/gene="BCK1"
/locus_tag="YJL095W"
/gene_synonym="LAS3; SAP3; SLK1; SSP31"
/product="mitogen-activated protein kinase kinase kinase
BCK1"
/transcript_id="NM_001181528.1"
/db_xref="GeneID:853350"
CDS 247255..251691
/gene="BCK1"
/locus_tag="YJL095W"
/gene_synonym="LAS3; SAP3; SLK1; SSP31"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807]"
/experiment="EXISTENCE:genetic interaction:GO:0000196 cell
integrity MAPK cascade [PMID:8386320|PMID:1729597]"
/experiment="EXISTENCE:genetic interaction:GO:0060237
regulation of fungal-type cell wall organization
[PMID:1729597]"
/experiment="EXISTENCE:mutant phenotype:GO:0000425
pexophagy [PMID:20385774]"
/experiment="EXISTENCE:mutant phenotype:GO:0004709 MAP
kinase kinase kinase activity [PMID:11283616]"
/experiment="EXISTENCE:mutant phenotype:GO:0010447
response to acidic pH [PMID:16087742]"
/experiment="EXISTENCE:mutant phenotype:GO:0030010
establishment of cell polarity [PMID:1545797]"
/experiment="EXISTENCE:mutant phenotype:GO:0030968
endoplasmic reticulum unfolded protein response
[PMID:16380504]"
/experiment="EXISTENCE:mutant phenotype:GO:0035556
intracellular signal transduction [PMID:8190082]"
/experiment="EXISTENCE:mutant phenotype:GO:0060237
regulation of fungal-type cell wall organization
[PMID:1729597]"
/note="MAPKKK acting in the protein kinase C signaling
pathway; the kinase C signaling pathway controls cell
integrity; upon activation by Pkc1p phosphorylates
downstream kinases Mkk1p and Mkk2p; MAPKKK is an acronym
for mitogen-activated protein (MAP) kinase kinase kinase"
/codon_start=1
/product="mitogen-activated protein kinase kinase kinase
BCK1"
/protein_id="NP_012440.1"
/db_xref="GeneID:853350"
/db_xref="SGD:S000003631"
/translation="MPFLRKIAGTAHTHSRSDSNSSVKFGHQPTSSVASTKSSSKSPR
ATSRKSIYDDIRSQFPNLTPNSTSSQFYESTPVIEQSFNWTTDDHISAGTLENPTSFT
NSSYKNDNGPSSLSDSRKSSGGNSVNSLSFDKLILSWDPTDPDEWTMHRVTSWFKFHD
FPESWILFFKKHQLFGHRFIKLLAYDNFAVYEKYLPQTKTASYTRFQQLLKKTMTKNV
TNSHIRQKSASKLKSSRSSSESIKSKLKNSKSQEDISNSRSTSESALSPTKSGPSKTD
EKNFLHSTSTHQKTKSASSLYRRSFISLRGSSSSNASSAKSPSNIKLSIPARPHSIIE
SNSTLTKSASPPASPSYPSIFRRHHKSSSSESSLLNSLFGSGIGEEAPTKPNPQGHSL
SSENLAKGKSKHYETNVSSPLKQSSLPTSDDKGNLWNKFKRKSQIGVPSPNTVAYVTS
QETPSLKSNSSTATLTVQTADVNIPSPSSSPPPIPKTANRSLEVISTEDTPKISSTTA
SFKETYPDCINPDKTVPVPVNNQKYSVKNFLLDQKFYPLKKTGLNDSENKYILVTKDN
VSFVPLNLKSVAKLSSFKESALTKLGINHKNVTFHMTDFDCDIGAAIPDDTLEFLKKS
LFLNTSGKIYIKDQMKLQQKPKPAPLTSENNVPLKSVKSKSSMRSGTSSLIASTDDVS
IVTSSSDITSFDEHASGSGRRYPQTPSYYYDRVSNTNPTEELNYWNIKEVLSHEENAP
KMVFKTSPKLELNLPDKGSKLNIPTPITENESKSSFQVLRKDEGTEIDFNHRRESPYT
KPELAPKREAPKPPANTSPQRTLSTSKQNKPIRLVRASTKISRSKRSKPLPPQLLSSP
IEASSSSPDSLTSSYTPASTHVLIPQPYKGANDVMRRLKTDQDSTSTSPSLKMKQKVN
RSNSTVSTSNSIFYSPSPLLKRGNSKRVVSSTSAADIFEENDITFADAPPMFDSDDSD
DDSSSSDDIIWSKKKTAPETNNENKKDEKSDNSSTHSDEIFYDSQTQDKMERKMTFRP
SPEVVYQNLEKFFPRANLDKPITEGIASPTSPKSLDSLLSPKNVASSRTEPSTPSRPV
PPDSSYEFIQDGLNGKNKPLNQAKTPKRTKTIRTIAHEASLARKNSVKLKRQNTKMWG
TRMVEVTENHMVSINKAKNSKGEYKEFAWMKGEMIGKGSFGAVYLCLNVTTGEMMAVK
QVEVPKYSSQNEAILSTVEALRSEVSTLKDLDHLNIVQYLGFENKNNIYSLFLEYVAG
GSVGSLIRMYGRFDEPLIKHLTTQVLKGLAYLHSKGILHRDMKADNLLLDQDGICKIS
DFGISRKSKDIYSNSDMTMRGTVFWMAPEMVDTKQGYSAKVDIWSLGCIVLEMFAGKR
PWSNLEVVAAMFKIGKSKSAPPIPEDTLPLISQIGRNFLDACFEINPEKRPTANELLS
HPFSEVNETFNFKSTRLAKFIKSNDKLNSSKLRITSQENKTE"
gene complement(<251821..>254442)
/gene="KHA1"
/locus_tag="YJL094C"
/db_xref="GeneID:853351"
mRNA complement(<251821..>254442)
/gene="KHA1"
/locus_tag="YJL094C"
/product="Kha1p"
/transcript_id="NM_001181527.1"
/db_xref="GeneID:853351"
CDS complement(251821..254442)
/gene="KHA1"
/locus_tag="YJL094C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:15926887]"
/experiment="EXISTENCE:mutant phenotype:GO:0015386
potassium:proton antiporter activity [PMID:9811642]"
/experiment="EXISTENCE:mutant phenotype:GO:0055085
transmembrane transport [PMID:9811642]"
/note="Putative K+/H+ antiporter; involved in
intracellular cation homeostasis; promotes copper binding
to Fet3p multicopper oxidase; localized to Golgi vesicles
and detected in highly purified mitochondria in
high-throughput studies"
/codon_start=1
/product="Kha1p"
/protein_id="NP_012441.1"
/db_xref="GeneID:853351"
/db_xref="SGD:S000003630"
/translation="MANTVGGILSGVNPFHYNSSSPLTLFLFQACLILLVCNLIHIPF
SMMRQPKVISEVISGVILGPTIFGQIPNYTNTIFPTSSIPGLNLVANLGIILFMFFLG
LEVDIAFIKKHLKKALVIGIVTLAVPFGFGCLLAIPLFHTYANKTEGERHIKFSVFMV
FIAVSISVTAFPVLCRILNELRLIKDRAGIVVLAAGIINDIMGWILLALSIILSSAEG
SPVNTVYILLITFAWFLIYFFPLKYLLRWVLIRTHELDRSKPSPLATMCILFIMFISA
YFTDIIGVHPIFGAFIAGLVVPRDDHYVVKLTERMEDIPNIVFIPIYFAVAGLNVDLT
LLNEGRDWGYVFATIGIAIFTKIISGTLTAKLTGLFWREATAAGVLMSCKGIVEIVVL
TVGLNAGIISRKIFGMFVLMALVSTFVTTPLTQLVYPDSYRDGVRKSLSTPAEDDGAA
DGLDSEGVDKTEINTQLNSLADVSKYRIGELTTVINTTEAISPSLKLLNYLSLGVSPK
PKNNKHKNETSLSRMTTATDSTLKSNTFKIKKMVHIWSKSVDDVDTNLSVIDEKLTPF
EGVGALRAIHLRLLTERTTDLLQSSSLYNDDPHFTANTDSLLQIFDIFSNLSKIPFSS
EVIFSTMREKAANIATMKMDSTDLILLPLKGASYEYRGSPVFIDEKYANFDHIYSHLL
GLNELSSTFFKSIFQSLKANFAVQISNTYGRLNADRFKRKRFNLLLPKPYLTQSDYLG
LYLLLLICYRDGYNNDNASCSIFINSKNIDFAKDLSTAFAEHDWLNESTIKIVDIPFE
TKVPEEAIEKPSFIETVLDVGLSDTALADIEETTFIIGEDLPDESEPFSEEVRTVIFE
GSNRRFDTLIVHHFSSE"
gene complement(<254737..>256812)
/gene="TOK1"
/locus_tag="YJL093C"
/gene_synonym="DUK1; YKC1; YPK1"
/db_xref="GeneID:853352"
mRNA complement(<254737..>256812)
/gene="TOK1"
/locus_tag="YJL093C"
/gene_synonym="DUK1; YKC1; YPK1"
/product="Tok1p"
/transcript_id="NM_001181526.1"
/db_xref="GeneID:853352"
CDS complement(254737..256812)
/gene="TOK1"
/locus_tag="YJL093C"
/gene_synonym="DUK1; YKC1; YPK1"
/experiment="EXISTENCE:direct assay:GO:0005267 potassium
channel activity [PMID:7651518]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:7651518]"
/experiment="EXISTENCE:direct assay:GO:0030007
intracellular potassium ion homeostasis [PMID:7651518]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0005249
voltage-gated potassium channel activity [PMID:16133265]"
/experiment="EXISTENCE:mutant phenotype:GO:0005267
potassium channel activity [PMID:7651518]"
/experiment="EXISTENCE:mutant phenotype:GO:0005886 plasma
membrane [PMID:7651518]"
/experiment="EXISTENCE:mutant phenotype:GO:0030007
intracellular potassium ion homeostasis [PMID:7651518]"
/note="Outward-rectifier potassium channel of the plasma
membrane; has two pore domains in tandem, each of which
forms a functional channel permeable to potassium; carboxy
tail functions to prevent inner gate closures; target of
K1 toxin"
/codon_start=1
/product="Tok1p"
/protein_id="NP_012442.1"
/db_xref="GeneID:853352"
/db_xref="SGD:S000003629"
/translation="MTRFMNSFAKQTLGYGNMATVEQESSAQAVDSHSNNTPKQAKGV
LAEELKDALRFRDERVSIINAEPSSTLFVFWFVVSCYFPVITACLGPVANTISIACVV
EKWRSLKNNSVVTNPRSNDTDVLMNQVKTVFDPPGIFAVNIISLVLGFTSNIILMLHF
SKKLTYLKSQLINITGWTIAGGMLLVDVIVCSLNDMPSIYSKTIGFWFACISSGLYLV
CTIILTIHFIGYKLGKYPPTFNLLPNERSIMAYTVLLSLWLIWGAGMFSGLLHITYGN
ALYFCTVSLLTVGLGDILPKSVGAKIMVLIFSLSGVVLMGLIVFMTRSIIQKSSGPIF
FFHRVEKGRSKSWKHYMDSSKNLSEREAFDLMKCIRQTASRKQHWFSLSVTIAIFMAF
WLLGALVFKFAENWSYFNCIYFCFLCLLTIGYGDYAPRTGAGRAFFVIWALGAVPLMG
AILSTVGDLLFDISTSLDIKIGESFNNKVKSIVFNGRQRALSFMVNTGEIFEESDTAD
GDLEENTTSSQSSQISEFNDNNSEENDSGVTSPPASLQESFSSLSKASSPEGILPLEY
VSSAEYALQDSGTCNLRNLQELLKAVKKLHRICLADKDYTLSFSDWSYIHKLHLRNIT
DIEEYTRGPEFWISPDTPLKFPLNEPHFAFMMLFKNIEELVGNLVEDEELYKVISKRK
FLGEHRKTL"
gene <257423..>260947
/gene="SRS2"
/locus_tag="YJL092W"
/gene_synonym="HPR5"
/db_xref="GeneID:853353"
mRNA <257423..>260947
/gene="SRS2"
/locus_tag="YJL092W"
/gene_synonym="HPR5"
/product="DNA helicase SRS2"
/transcript_id="NM_001181525.1"
/db_xref="GeneID:853353"
CDS 257423..260947
/gene="SRS2"
/locus_tag="YJL092W"
/gene_synonym="HPR5"
/EC_number="5.6.2.4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:19506039]"
/experiment="EXISTENCE:direct assay:GO:0006303
double-strand break repair via nonhomologous end joining
[PMID:10908335]"
/experiment="EXISTENCE:direct assay:GO:0008047 enzyme
activator activity [PMID:25765656]"
/experiment="EXISTENCE:direct assay:GO:0043138 3'-5' DNA
helicase activity [PMID:8419328]"
/experiment="EXISTENCE:direct assay:GO:1990986 DNA
recombinase disassembly [PMID:12748644|PMID:12748645]"
/experiment="EXISTENCE:direct assay:GO:2000042 negative
regulation of double-strand break repair via homologous
recombination [PMID:19595720]"
/experiment="EXISTENCE:genetic interaction:GO:0006281 DNA
repair [PMID:7768432]"
/experiment="EXISTENCE:mutant phenotype:GO:0007127 meiosis
I [PMID:23770697]"
/experiment="EXISTENCE:mutant phenotype:GO:0042262 DNA
protection [PMID:31168653]"
/experiment="EXISTENCE:mutant phenotype:GO:0045910
negative regulation of DNA recombination [PMID:23770697]"
/experiment="EXISTENCE:mutant phenotype:GO:1990986 DNA
recombinase disassembly [PMID:23770697]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:31142613]"
/note="DNA helicase and DNA-dependent ATPase; role in DNA
repair and checkpoint recovery, timely commitment to
meiotic recombination and the MI to MII transition; blocks
trinucleotide repeat expansion; disassembles Rad51p
nucleoprotein filaments during meiotic recombination;
stimulates Mus81p-Mms4p endonuclease activity; delays
abscission in the presence of chromatin bridges to prevent
chromosome breakage during cytokinesis; ATP hydrolysis
activity inhibited by Dmc1p; functional homolog of human
RTEL1"
/codon_start=1
/product="DNA helicase SRS2"
/protein_id="NP_012443.1"
/db_xref="GeneID:853353"
/db_xref="SGD:S000003628"
/translation="MSSNNDLWLHLVSQLNTQQRAAALFDYTRGLQVIAGPGTGKTKV
LTSRVAYLILHHHIHPRDIIVTTFTNKAANEMKERLQEMLRGAGVNISELLIGTFHSI
CLKILYRFGHLVDLQKDWRIIDEKEIDVILDDMIEKVPDQIRDYASSITRKVNLCMPS
KNGDEWTIHPKLIKKQISKLKSNAILPEEYILDSNHDAALGYFYQIYQSELSKKNTLD
FDDLLMYTFRLLTRVRVLSNIKHVLVDEFQDTNGIQLDLMFLFAKGNHHLSRGMTIVG
DPDQSIYAFRNALAHNFLEMGRKCPIEYSTIILVENYRSSQKILNTSEILITQQNKGR
QNRAPLRAQFDLDFPPVYMNFPAYFLEAPSLVRELLYLKALPNLFTFNDFAILVRQRR
QIKRIESALIEHRIPYKIIRGHSFWDSKETRAMLNLLKLIFSPNDKHAILASLLYPAR
GLGPATGEKIKNALDTLATDVSCFQILKDISSKKIMLDIPTKGRSVIADFISMIENCQ
LLLQSTLLGGLSDLFDKLYELSGLKYEYLYKDGKKKNDQLEKSEPNLLNARHKNIELL
KNYFLALLSKSESSDKEKNEAIKAATDEAEPIENKVITPKEYLRNFFNSLSLHSDAAE
EEESESNKDAKIKREKNGFVTISTIHGAKGLEWPVVFIPGCEEGIIPCVFNDDKKDES
EEDEEEDQENSKKDASPKKTRVLSVEDSIDEERRMFFVAQTRAKYLLYLSNTVTVEDV
DRPRIASRFLTTDLIKAMSDSQKLFESTNSIKKLYRILNKKPPAEDDKLFSLDQLRKD
YNQFIENRRERMIWQGIQMNDVYGIQLSRNKLLGSVSDFTSAADQLRLETQNSIFPQK
KLIEKSRPSKINGNYAPKSRVKSPEKRYAPETTSFHSPTKKKVYAPQYVSTTNVPSRQ
EFHSSTGKNIPFLRREDRSITDISPRSSTRSLKGASPNKTSHMSDDLMRPSPTRKDKV
TRNIHFATAGTFRIETQSNVDELHPPEYSNKSGQSLTSSEFSGFSSACSNSDQPTNLI
EDINNELDLSDEELLNDISIERRRELLGSKKTKKIKPKTRNRKSKRGDKVKVEEVIDL
KSEFEEDDSRNTTAAELLHNPDDTTVDNRPIISNAKFLADAAMKKTQKFSKKVKNEPA
SSQMDIFSQLSRAKKKSKLNNGEIIVID"
gene complement(<261080..>262552)
/gene="GWT1"
/locus_tag="YJL091C"
/db_xref="GeneID:853354"
mRNA complement(<261080..>262552)
/gene="GWT1"
/locus_tag="YJL091C"
/product="glucosaminyl-phosphotidylinositol
O-acyltransferase"
/transcript_id="NM_001181524.1"
/db_xref="GeneID:853354"
CDS complement(261080..262552)
/gene="GWT1"
/locus_tag="YJL091C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0006506 GPI anchor
biosynthetic process [PMID:12714589]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:21367863]"
/experiment="EXISTENCE:direct assay:GO:0042175 nuclear
outer membrane-endoplasmic reticulum membrane network
[PMID:14690591]"
/experiment="EXISTENCE:genetic interaction:GO:0032216
glucosaminyl-phosphatidylinositol O-acyltransferase
activity [PMID:14517336]"
/experiment="EXISTENCE:mutant phenotype:GO:0032216
glucosaminyl-phosphatidylinositol O-acyltransferase
activity [PMID:16361252]"
/note="Protein involved in the inositol acylation of
GlcN-PI; the inositol acylation of glucosaminyl
phosphatidylinositol (GlcN-PI) forms
glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an
intermediate in the biosynthesis of
glycosylphosphatidylinositol (GPI) anchors"
/codon_start=1
/product="glucosaminyl-phosphotidylinositol
O-acyltransferase"
/protein_id="NP_012444.2"
/db_xref="GeneID:853354"
/db_xref="SGD:S000003627"
/translation="MSTLKQRKEDFVTGLNGGSITEINAVTSIALVTYISWNLLKNSN
LMPPGISSVQYIIDFALNWVALLLSITIYASEPYLLNTLILLPCLLAFIYGKFTSSSK
PSNPIYNKKKMITQRFQLEKKPYITAYRGGMLILTAIAILAVDFPIFPRRFAKVETWG
TSLMDLGVGSFVFSNGIVSSRALLKNLSLKSKPSFLKNAFNALKSGGTLLFLGLLRLF
FVKNLEYQEHVTEYGVHWNFFITLSLLPLVLTFIDPVTRMVPRCSIAIFISCIYEWLL
LKDDRTLNFLILADRNCFFSANREGIFSFLGYCSIFLWGQNTGFYLLGNKPTLNNLYK
PSTQDVVAASKKSSTWDYWTSVTPLSGLCIWSTIFLVISQLVFQYHPYSVSRRFANLP
YTLWVITYNLLFLTGYCLTDKIFGNSSEYYKVAECLESINSNGLFLFLLANVSTGLVN
MSMVTIDSSPLKSFLVLLAYCSFIAVISVFLYRKRIFIKL"
gene complement(<262757..>265051)
/gene="DPB11"
/locus_tag="YJL090C"
/db_xref="GeneID:853355"
mRNA complement(<262757..>265051)
/gene="DPB11"
/locus_tag="YJL090C"
/product="protein kinase activating protein DPB11"
/transcript_id="NM_001181523.1"
/db_xref="GeneID:853355"
CDS complement(262757..265051)
/gene="DPB11"
/locus_tag="YJL090C"
/experiment="EXISTENCE:direct assay:GO:0000725
recombinational repair [PMID:16840526]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005657 replication
fork [PMID:10733584]"
/experiment="EXISTENCE:direct assay:GO:0030295 protein
kinase activator activity [PMID:18922789]"
/experiment="EXISTENCE:direct assay:GO:0070182 DNA
polymerase binding [PMID:10733584]"
/experiment="EXISTENCE:genetic interaction:GO:0000725
recombinational repair [PMID:16840526]"
/experiment="EXISTENCE:genetic interaction:GO:0007095
mitotic G2 DNA damage checkpoint signaling
[PMID:18541674]"
/experiment="EXISTENCE:mutant phenotype:GO:0000076 DNA
replication checkpoint signaling [PMID:10097122]"
/experiment="EXISTENCE:mutant phenotype:GO:0000727
double-strand break repair via break-induced replication
[PMID:20516198]"
/experiment="EXISTENCE:mutant phenotype:GO:0006270 DNA
replication initiation [PMID:10733584|PMID:23629628]"
/experiment="EXISTENCE:mutant phenotype:GO:0007533 mating
type switching [PMID:21130053]"
/experiment="EXISTENCE:mutant phenotype:GO:0031261 DNA
replication preinitiation complex [PMID:17167415]"
/note="DNA replication initiation protein; loads DNA pol
epsilon onto pre-replication complexes at origins;
checkpoint sensor recruited to stalled replication forks
by the checkpoint clamp complex where it activates Mec1p;
along with Rfa1p, binds to ultrafine anaphase bridges in
mitotic cells and prevents accumulation of chromatin
bridges by stimulating the Mec1p kinase and suppressing
homologous recombination; ortholog of human TopBP1; forms
nuclear foci upon DNA replication stress"
/codon_start=1
/product="protein kinase activating protein DPB11"
/protein_id="NP_012445.1"
/db_xref="GeneID:853355"
/db_xref="SGD:S000003626"
/translation="MKPFQGITFCPTAINNEILAKKISKKIIKLGGIFSKDLTRQVNV
LVVGSTTNTNKFKFAVKHRFDIIFIDIQAIDDIYQLWLSGENILPDSNTATMTGSTYE
MLKILYRRFSFKYLHNFNIFIGRITDTNITSIDSLVRSIKKLGCSSYNYQNFVIKDTS
SHNDDDDQGQNGQISIFVTDTLLGARVNAAIEQNIPIVHFKWILDCQKRSALLPYDPY
YLLPNIKDLPYDSIGSNSCDCWDKINTTFPTNIDAQSSLQRQQSSSTLTPSLPKTSSL
LNKFKPKGEKIWDKAMSLQQHSKTNFSVLGQSPLSINNKQEDLSDNSTLIFKNCAFII
HHIFPGNHRSILTKIVVQNGGKIETSYLSGIYDHSYYIIPSNKALDSFNDLPEIIDDN
DGIVTEFFIERCLYYQKLLHPIDLWSKPFLSTIEFQVSSSSKLLHHEFSSSPFLNVTI
TGFSGVELLHLTKVLNLLKPMGINYVEYLNKSTDILLINLAALPSIPKTHPLWSNEFS
DLFTQFCINNNNDDPGDNNRKDFQNNSILRNSMKRKIEYIKKFHSIPVVTPAFIFKLL
SAASGENNEIFLNNIKWCIICPRGHKDDFKCKIKKPYYTSISSEKKYQNNDPKIDKTI
LLKRNNSSLSEHSMKDTKNELLQKIRETDSGRKKRSVSSSIMDVSSERQMPDTKRIKL
ESLPKNFVPKQIKRTTSWGTIMSENVPTEQPTAISNPEEIPRTEEVSHTQVTYGSIQD
KKRTASLEKPMRRQTRNQTKELDS"
gene <265926..>268415
/gene="SIP4"
/locus_tag="YJL089W"
/db_xref="GeneID:853356"
mRNA <265926..>268415
/gene="SIP4"
/locus_tag="YJL089W"
/product="Sip4p"
/transcript_id="NM_001181522.1"
/db_xref="GeneID:853356"
CDS 265926..268415
/gene="SIP4"
/locus_tag="YJL089W"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:9843506|PMID:14685767|PMID:11405098]"
/experiment="EXISTENCE:direct assay:GO:0001228 DNA-binding
transcription activator activity, RNA polymerase
II-specific [PMID:14685767]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11331606|PMID:9843506]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:19111667]"
/experiment="EXISTENCE:genetic interaction:GO:0045722
positive regulation of gluconeogenesis [PMID:25123508]"
/experiment="EXISTENCE:genetic interaction:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:25123508]"
/experiment="EXISTENCE:mutant phenotype:GO:0045722
positive regulation of gluconeogenesis [PMID:9843506]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:9843506]"
/experiment="EXISTENCE:mutant phenotype:GO:2000218
negative regulation of invasive growth in response to
glucose limitation [PMID:11095711]"
/experiment="EXISTENCE:physical interaction:GO:0045722
positive regulation of gluconeogenesis [PMID:9843506]"
/note="C6 zinc cluster transcriptional activator; binds to
the carbon source-responsive element (CSRE) of
gluconeogenic genes; involved in the positive regulation
of gluconeogenesis; regulated by Snf1p protein kinase;
localized to the nucleus"
/codon_start=1
/product="Sip4p"
/protein_id="NP_012446.1"
/db_xref="GeneID:853356"
/db_xref="SGD:S000003625"
/translation="MAKRKYGRSYSLDDTDSCSNKVLIVPTGQSSSNAITDFSVRKAH
ACDRCRLKKIKCDGLKPNCSNCAKIDFPCKTSDKLSRRGLPKGYTELLEKEVVRLTNM
NASSSANANSNLPFINDTFYCFDNYNTQSENQRFLGHLTWNILTNTFPTQKAVVFTDD
RNNIDLQLQLLTNFLNLNGDFNHLPNFLLLKYDYNLQFLKNLLSVIIKDFFKRQNSLL
LLLYPTNLWKNLLLDKINSTAMTGEPITLLALLYIIQFTWSCFDDFKLFKVTKLIVSL
TTNSKLDLKVLQLVNLSIFYFMGASVDSCKSKSSLTEHSNVNSVIWTNDLLNLNFTNI
LNMGLYINPKNLIPISGNNNNNKSNEEDDRIVTFWCFQFLSSWWSLIQGLPKSNFLTE
EFQPKSISVLEIPRLKPFEILLNFIIYSLDGCNLLNISSLNVSDPNFQFFQNELESFK
KNLLLWNLYHNLSDHDNFRFLTSSSNKKLTTNLLLKNLTGLNHKLNQPDFVEIQLTLF
YLSLKLMTLKEGDQDLKKEDISLEILSLYFLILTDDSNNDDNQQLQPQQLNLYHFTPF
NSIDIIDLCLNNLNNWSLSLKYESGQNQPHSSKIKFEKFQNFLNHWCPIWYYDEFSTN
PFLQILKINFKLLPFETIHYSQEEQRLLISLNKLRYLDAVSSFNSSSVKSNFASKVNT
QLNLLQHSSSNSNFLDASPYDFNKIFMNNFENYDYETDEGYAEDDDEEDSDSDNSLPL
EIPFKKSKNKCKNRNKELSQRLSLFENRDSNSVDFNTDTNLNLNPDSPSVTSSKKKYL
DHIILDNRDIVSNHDSSKQKFKIQNILNSTF"
gene <268799..>269815
/gene="ARG3"
/locus_tag="YJL088W"
/db_xref="GeneID:853357"
mRNA <268799..>269815
/gene="ARG3"
/locus_tag="YJL088W"
/product="ornithine carbamoyltransferase"
/transcript_id="NM_001181521.1"
/db_xref="GeneID:853357"
CDS 268799..269815
/gene="ARG3"
/locus_tag="YJL088W"
/EC_number="2.1.3.3"
/experiment="EXISTENCE:direct assay:GO:0004585 ornithine
carbamoyltransferase activity [PMID:7029528]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:205532]"
/experiment="EXISTENCE:mutant phenotype:GO:0006526
L-arginine biosynthetic process [PMID:2897249]"
/note="Ornithine carbamoyltransferase; also known as
carbamoylphosphate:L-ornithine carbamoyltransferase;
catalyzes the biosynthesis of the arginine precursor
citrulline"
/codon_start=1
/product="ornithine carbamoyltransferase"
/protein_id="NP_012447.1"
/db_xref="GeneID:853357"
/db_xref="SGD:S000003624"
/translation="MSTTASTPSSLRHLISIKDLSDEEFRILVQRAQHFKNVFKANKT
NDFQSNHLKLLGRTIALIFTKRSTRTRISTEGAATFFGAQPMFLGKEDIQLGVNESFY
DTTKVVSSMVSCIFARVNKHEDILAFCKDSSVPIINSLCDKFHPLQAICDLLTIIENF
NISLDEVNKGINSKLKMAWIGDANNVINDMCIACLKFGISVSISTPPGIEMDSDIVDE
AKKVAERNGATFELTHDSLKASTNANILVTDTFVSMGEEFAKQAKLKQFKGFQINQEL
VSVADPNYKFMHCLPRHQEEVSDDVFYGEHSIVFEEAENRLYAAMSAIDIFVNNKGNF
KDLK"
gene complement(<270002..>272485)
/gene="TRL1"
/locus_tag="YJL087C"
/gene_synonym="LIG1; RLG1"
/db_xref="GeneID:853358"
mRNA complement(<270002..>272485)
/gene="TRL1"
/locus_tag="YJL087C"
/gene_synonym="LIG1; RLG1"
/product="tRNA ligase"
/transcript_id="NM_001181520.1"
/db_xref="GeneID:853358"
CDS complement(270002..272485)
/gene="TRL1"
/locus_tag="YJL087C"
/gene_synonym="LIG1; RLG1"
/EC_number="6.5.1.3"
/experiment="EXISTENCE:direct assay:GO:0003972 RNA ligase
(ATP) activity [PMID:3512545|PMID:3277966]"
/experiment="EXISTENCE:direct assay:GO:0004113
2',3'-cyclic-nucleotide 3'-phosphodiesterase activity
[PMID:6297798]"
/experiment="EXISTENCE:direct assay:GO:0005637 nuclear
inner membrane [PMID:3312232]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:20844078]"
/experiment="EXISTENCE:direct assay:GO:0006388 tRNA
splicing, via endonucleolytic cleavage and ligation
[PMID:2207062]"
/experiment="EXISTENCE:direct assay:GO:0051730
GTP-dependent polyribonucleotide 5'-hydroxyl-kinase
activity [PMID:8428918]"
/experiment="EXISTENCE:mutant phenotype:GO:0006388 tRNA
splicing, via endonucleolytic cleavage and ligation
[PMID:1537841]"
/experiment="EXISTENCE:mutant phenotype:GO:0032056
positive regulation of translation in response to stress
[PMID:20844078]"
/experiment="EXISTENCE:mutant phenotype:GO:0036498
IRE1-mediated unfolded protein response [PMID:8898194]"
/experiment="EXISTENCE:mutant phenotype:GO:0070966
nuclear-transcribed mRNA catabolic process, no-go decay
[PMID:31913314]"
/experiment="EXISTENCE:mutant phenotype:GO:2000622
regulation of nuclear-transcribed mRNA catabolic process,
nonsense-mediated decay [PMID:30874502]"
/note="tRNA ligase; required for tRNA splicing and for
both splicing and translation of HAC1 mRNA in the UPR; has
phosphodiesterase, polynucleotide kinase, and ligase
activities; involved in turnover of tRNA introns;
localized at the inner nuclear envelope and cytoplasm"
/codon_start=1
/product="tRNA ligase"
/protein_id="NP_012448.1"
/db_xref="GeneID:853358"
/db_xref="SGD:S000003623"
/translation="MPSPYDGKRTVTQLVNELEKAEKLSGRGRAYRRVCDLSHSNKKV
ISWKFNEWDYGKNTITLPCNARGLFISDDTTNPVIVARGYDKFFNVGEVNFTKWNWIE
ENCTGPYDVTIKANGCIIFISGLEDGTLVVCSKHSTGPRADVDRNHAEAGEKQLLRQL
AAMNINRSDFARMLYTHNVTAVAEYCDDSFEEHILEYPLEKAGLYLHGVNVNKAEFET
WDMKDVSQMASKYGFRCVQCITSNTLEDLKKFLDNCSATGSFEGQEIEGFVIRCHLKS
TEKPFFFKYKFEEPYLMYRQWREVTKDYISNKSRVFKFRKHKFITNKYLDFAIPILES
SPKICENYLKGFGVIELRNKFLQSYGMSGLEILNHEKVAELELKNAIDYDKVDERTKF
LIFPISVIGCGKTTTSQTLVNLFPDSWGHIQNDDITGKDKSQLMKKSLELLSKKEIKC
VIVDRNNHQFRERKQLFEWLNELKEDYLVYDTNIKVIGVSFAPYDKLSEIRDITLQRV
IKRGNNHQSIKWDELGEKKVVGIMNGFLKRYQPVNLDKSPDNMFDLMIELDFGQADSS
LTNAKQILNEIHKAYPILVPEIPKDDEIETAFRRSLDYKPTVRKIVGKGNNNQQKTPK
LIKPTYISAKIENYDEIIELVKRCIASDAELTEKFKHLLASGKVQKELHITLGHVMSS
REKEAKKLWKSYCNRYTDQITEYNNNRIENAQGSGNNQNTQVKTTDKLNFRLEKLCWD
EKIIAIVVELSKDKDGCIIDENNEKIKGLCCQNKIPHITLCKLESGVKAVYSNVLCEK
VESAEVDENIKVVKLDNSKEFVGSVYLNF"
gene <272827..>274698
/gene="EXO70"
/locus_tag="YJL085W"
/db_xref="GeneID:853359"
mRNA <272827..>274698
/gene="EXO70"
/locus_tag="YJL085W"
/product="GTP-Rho binding exocyst subunit EXO70"
/transcript_id="NM_001181518.1"
/db_xref="GeneID:853359"
CDS 272827..274698
/gene="EXO70"
/locus_tag="YJL085W"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:18195105]"
/experiment="EXISTENCE:direct assay:GO:0000145 exocyst
[PMID:8978675]"
/experiment="EXISTENCE:direct assay:GO:0005546
phosphatidylinositol-4,5-bisphosphate binding
[PMID:17717527]"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:17717527]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip
[PMID:22842922|PMID:18946089|PMID:17717527|PMID:10207081]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:22842922|PMID:18946089|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0007266 Rho protein
signal transduction [PMID:19955214]"
/experiment="EXISTENCE:direct assay:GO:0031267 small
GTPase binding [PMID:10588647]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807]"
/experiment="EXISTENCE:genetic interaction:GO:0006887
exocytosis [PMID:17717527|PMID:15583030]"
/experiment="EXISTENCE:genetic interaction:GO:0007266 Rho
protein signal transduction [PMID:19955214]"
/experiment="EXISTENCE:genetic interaction:GO:0051601
exocyst localization [PMID:17717527|PMID:18946089]"
/experiment="EXISTENCE:mutant phenotype:GO:0001927 exocyst
assembly [PMID:18946089|PMID:15583030]"
/experiment="EXISTENCE:mutant phenotype:GO:0006887
exocytosis [PMID:17339375]"
/experiment="EXISTENCE:mutant phenotype:GO:0006893 Golgi
to plasma membrane transport [PMID:17339375]"
/experiment="EXISTENCE:physical interaction:GO:0007266 Rho
protein signal transduction [PMID:19955214]"
/experiment="EXISTENCE:physical interaction:GO:0031267
small GTPase binding [PMID:10207081|PMID:19955214]"
/note="Subunit of the exocyst complex; the exocyst
mediates polarized targeting and tethering of post-Golgi
secretory vesicles to active sites of exocytosis prior to
SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that
localizes to exocytic sites in an actin-independent
manner, targeting and anchoring the exocyst with Sec3p;
involved in exocyst assembly; direct downstream effector
of Rho3p and Cdc42p; relocalizes from bud neck to
cytoplasm upon DNA replication stress"
/codon_start=1
/product="GTP-Rho binding exocyst subunit EXO70"
/protein_id="NP_012450.1"
/db_xref="GeneID:853359"
/db_xref="SGD:S000003621"
/translation="MPAEIDIDEADVLVLSQELQKTSKLTFEINKSLKKIAATSNQSS
QLFTPILARNNVLTTLQRNIESTLNSVASVKDLANEASKYEIILQKGINQVGLKQYTQ
VVHKLDDMLEDIQSGQANREENSEFHGILTHLEQLIKRSEAQLRVYFISILNSIKPFD
PQINITKKMPFPYYEDQQLGALSWILDYFHGNSEGSIIQDILVGERSKLILKCMAFLE
PFAKEISTAKNAPYEKGSSGMNSYTEALLGFIANEKSLVDDLYSQYTESKPHVLSQIL
SPLISAYAKLFGANLKIVRSNLENFGFFSFELVESINDVKKSLRGKELQNYNLLQDCT
QEVRQVTQSLFRDAIDRIIKKANSISTIPSNNGVTEATVDTMSRLRKFSEYKNGCLGA
MDNITRENWLPSNYKEKEYTLQNEALNWEDHNVLLSCFISDCIDTLAVNLERKAQIAL
MPNQEPDVANPNSSKNKHKQRIGFFILMNLTLVEQIVEKSELNLMLAGEGHSRLERLK
KRYISYMVSDWRDLTANLMDSVFIDSSGKKSKDKEQIKEKFRKFNEGFEDLVSKTKQY
KLSDPSLKVTLKSEIISLVMPMYERFYSRYKDSFKNPRKHIKYTPDELTTVLNQLVR"
gene complement(<274862..>278002)
/gene="ALY2"
/locus_tag="YJL084C"
/gene_synonym="ART3"
/db_xref="GeneID:853361"
mRNA complement(<274862..>278002)
/gene="ALY2"
/locus_tag="YJL084C"
/gene_synonym="ART3"
/product="Aly2p"
/transcript_id="NM_001181517.1"
/db_xref="GeneID:853361"
CDS complement(274862..278002)
/gene="ALY2"
/locus_tag="YJL084C"
/gene_synonym="ART3"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005769 early
endosome [PMID:20739461]"
/experiment="EXISTENCE:direct assay:GO:0005770 late
endosome [PMID:20739461]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0051453
regulation of intracellular pH [PMID:38248315]"
/experiment="EXISTENCE:mutant phenotype:GO:0032386
regulation of intracellular transport [PMID:20739461]"
/experiment="EXISTENCE:mutant phenotype:GO:0070086
ubiquitin-dependent endocytosis [PMID:32800549]"
/experiment="EXISTENCE:mutant phenotype:GO:1903577
cellular response to L-arginine [PMID:32800549]"
/experiment="EXISTENCE:mutant phenotype:GO:2000397
positive regulation of ubiquitin-dependent endocytosis
[PMID:20956561]"
/experiment="EXISTENCE:physical interaction:GO:0030674
protein-macromolecule adaptor activity [PMID:20956561]"
/experiment="EXISTENCE:physical interaction:GO:0031625
ubiquitin protein ligase binding [PMID:18976803]"
/note="Alpha arrestin, Ub-ligase adaptor for Rsp5p;
controls nutrient limitation-mediated endosomal recycling
of the Gap1p amino acid permease; PY motif-containing
adaptor involved in substrate-induced, Rsp5p-mediated
ubiquitination and endocytosis of select plasma membrane
localized amino acid transporters, such as Dip5p and
Put4p; regulates intracellular pH; phosphorylated by Npr1p
and by the Pcl7p-Pho85p cyclin-CDK complex; interacts with
AP-1 subunit Apl4p"
/codon_start=1
/product="Aly2p"
/protein_id="NP_012451.1"
/db_xref="GeneID:853361"
/db_xref="SGD:S000003620"
/translation="MPMDQSISSPLFPMEKDIDIPLDATPLAQSSSLQLFIHLAEPVV
FLQGFDPQKTEYPSVVLRGCLVVRILKPTKLKSISLSFKGYSRTEWPEGIPPKRQEFV
EIKDIVDHTWALYPPTEQKSKKKMDASAPNESNNAANNFLTKESGASLYRTLSDNETI
TSRKNSISGLSSLNLSPLGAPGNSSVNVKDRESRQRSRSSSVTSSNGPSRNLSPINLL
KRATSPSVSHHNYKPTTTSIFSDLLNNTFTHNDAASHHGHHIPTSSNHLAMTSNNFTS
GSGGEFFVFQPGDYIYAFEELIPQAYPESIKADFGFVEYFLFASIERPGAFKSNISAR
QVVNIVRTQAHNSVEESEPIIISRDWENQLYYDIVIASKDIILDAFLPITFKFAPLDK
VTLHRIRIYVTETMEYYCREKKVHRMEPTKKFLLTEQKGPKLPNLPNDANLSKAKNMG
NLLQDPKNGDLVNKEYEYQIFIPSRFNNHQQLHPDTSYENIKANHWIKICLRLSRVVD
NKRKHYEISIDSPIHVLHRLCSHANTLLPSYDGHPASFPKETDSSISSILESSDDNIN
LYHNSNIFFPKEVLSSPVLSPNVQPLDILIPHLPSTSLTRNSRQFNRNSKSHPSDNTI
FNSAKLKSNIYQPESLQRELASPQAIPLSPITSPMSNMEVPPPDFDFSSDFISDAASG
TTTTEVSSSESSILPRDPPSYKDTVLHDNNQKRRPNSKHPTPPSLKASHPNKNSDKNS
SETLNKKESMSKIEENKHKRETTPKKRENRDVKSLSTPQREESKDSTSTGNQSNEKNR
KRVLSLSSSLHSSPNNSGFAHSALGNLSNESLRSLNRRESVQDNLPSTIRHDNPFFTD
LNQVLIEDELKNHDKNELNRHSTNTSSTPASARSSFDYSGINISKDKLNMEPLLSKTE
TLTNKVNEDSFLRPNDSYVDLLEPSVDTTIDITAPYARNSSAWHPLQNDNDNNQFSPL
LGSNENFLNAANAQNSAESDHNNDIFTQGSGLTESSKNSDSEERFISRLSSPEKVLIN
TLDNESGLQSINESTL"
gene <278841..>280655
/gene="TAX4"
/locus_tag="YJL083W"
/db_xref="GeneID:853362"
mRNA <278841..>280655
/gene="TAX4"
/locus_tag="YJL083W"
/product="Tax4p"
/transcript_id="NM_001181516.1"
/db_xref="GeneID:853362"
CDS 278841..280655
/gene="TAX4"
/locus_tag="YJL083W"
/experiment="EXISTENCE:direct assay:GO:0000407 phagophore
assembly site [PMID:18298591]"
/experiment="EXISTENCE:genetic interaction:GO:0006914
autophagy [PMID:18298591]"
/experiment="EXISTENCE:genetic interaction:GO:0009267
cellular response to starvation [PMID:18298591]"
/experiment="EXISTENCE:genetic interaction:GO:0031505
fungal-type cell wall organization [PMID:15265867]"
/note="EH domain-containing protein; involved in
regulating phosphatidylinositol 4,5-bisphosphate levels
and autophagy; Irs4p and Tax4p bind and activate the
PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in
localizing Atg17p to the PAS; TAX4 has a paralog, IRS4,
that arose from the whole genome duplication"
/codon_start=1
/product="Tax4p"
/protein_id="NP_012452.1"
/db_xref="GeneID:853362"
/db_xref="SGD:S000003619"
/translation="MHFPKKKHSGNLSVVELPKEALQDSLTAAQITFKRYAHPNGNAG
SAERPRHLKVESAPVVKSEPSLPRMRQPEPRSINHQYSRETLPGHSEAFSVPTTPLQT
IHYDVRNKASNSPSSIAAAETAAYLAHTNSFSNRSSGVGSRDPVMDTETKPPRAPSAL
KNELQLNRMRIPPPSYDNNVRSRSISPQVSYSTSLSSSCSISSDGEETSYREKSTDEA
FPPEPSMSSYSLASKASAKASLTDPSQRQQESDYTAMNKLNGGNIIYKGTLPDLIPRS
QRKTSKPRFKHRLLRSPEQQQENLSRVYSDQTQNGRAIINTQQNVKLKTTMRRGKYAI
TDNDETFPYDRKSVSSDSDTDEDSNVMEIKDKKKKSRRSKIKKGLKTTAAVVGSSTSV
LPFPHHHHHHHQLHNPNSHHLHTHHHTSSHKFNEDKPWKSHRDLGFITEQERKRYESM
WVSNRYSYLRLLPWWPSLANEDDESHLQPLNLPQDGLMLNLVVKDIWYRSNLPRDLLV
QIYNMVDTRKDGTLDRKSFIVGMWLVDQCLYGRKLTNELDQRVWNSVDGYVLGTINVK
PATSDHYHNANNPLDKPSKLSVRQELKNIKRDLRNVRI"
gene <281185..>283380
/gene="IML2"
/locus_tag="YJL082W"
/db_xref="GeneID:853363"
mRNA <281185..>283380
/gene="IML2"
/locus_tag="YJL082W"
/product="Iml2p"
/transcript_id="NM_001181515.1"
/db_xref="GeneID:853363"
CDS 281185..283380
/gene="IML2"
/locus_tag="YJL082W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:16407407]"
/experiment="EXISTENCE:mutant phenotype:GO:0071218
cellular response to misfolded protein [PMID:26004510]"
/note="Protein required for clearance of inclusion bodies;
localizes to the inclusion bodies formed under protein
misfolding stress; the authentic, non-tagged protein is
detected in highly purified mitochondria in
high-throughput studies; protein abundance increases in
response to DNA replication stress; IML2 has a paralog,
YKR018C, that arose from the whole genome duplication"
/codon_start=1
/product="Iml2p"
/protein_id="NP_012453.1"
/db_xref="GeneID:853363"
/db_xref="SGD:S000003618"
/translation="MFRVFGSFGSKGNQSSGEEQSTKTKQVLKQANDFEIALKAMDFV
LDDRTDEGLNLLKKAEMETGSDQTILTLARGVIEFLQATLSFETEEMKRAAITLGKAE
QMSWKSKQNAEKTNFRSSSIYPPGTVYAVTYTESCLLHALLMLFSESMMEAAKALLKL
RRAYTMLQDIMVTVKKAERSKNSSSPSPSEKSQESCGSFVSAETTFISVDIPYKLSSE
DKSNPLLLEFAEKIYTMRMGRLSGAHIGNTPSFHRLRDDLGLQTTPSQASDRHSVSDD
FDLEQATIDEFIHSGANLCYGILQVVLSLLPPAIGAVLSIVGFKGSREEGLRLVWKAT
KERNVHGCIGLLGLMFYYDGPFQFTDADFDIPPNDNGSRALNKSRTNDSSLLPGYMDS
ATLLHPGKILEDALLKARALFPNSALWLLNEAKMLAGKGRLRDSLALMDSIDVNSIRM
RQVKSLMVFERAILLVNLHEYNRAADDLISLLDISDWSHALYTYFAGCCYLENWRMTQ
LGLLNDGKEQFYKERARELIFDAPSLLGKKTFKSKNLPLDRFMLRKVQQFNNMQKKLN
LQEPLDSIATSPVHELAYFYNGYNRMTENDLILTKKMLTEYHNPAIDSEDPDQELIRN
LLLSLTLRRLGDAERGLALLDDIVLPKIFYIQNGKVKYFKKTEDPWAYPAALYERALF
CWKLGGMESLNECREWLLRAQNYAADYELSTRIGMKIKAALDRVENALA"
gene complement(<283802..>285271)
/gene="ARP4"
/locus_tag="YJL081C"
/gene_synonym="ACT3"
/db_xref="GeneID:853364"
mRNA complement(<283802..>285271)
/gene="ARP4"
/locus_tag="YJL081C"
/gene_synonym="ACT3"
/product="Arp4p"
/transcript_id="NM_001181514.1"
/db_xref="GeneID:853364"
CDS complement(283802..285271)
/gene="ARP4"
/locus_tag="YJL081C"
/gene_synonym="ACT3"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:10436015]"
/experiment="EXISTENCE:direct assay:GO:0000812 Swr1
complex [PMID:14645854|PMID:16299513|PMID:14690608]"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:10436015]"
/experiment="EXISTENCE:direct assay:GO:0005524 ATP binding
[PMID:16088870]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10911987|PMID:11011149]"
/experiment="EXISTENCE:direct assay:GO:0006281 DNA repair
[PMID:16135807|PMID:12353039]"
/experiment="EXISTENCE:direct assay:GO:0006338 chromatin
remodeling [PMID:14645854]"
/experiment="EXISTENCE:direct assay:GO:0006357 regulation
of transcription by RNA polymerase II [PMID:10911987]"
/experiment="EXISTENCE:direct assay:GO:0035267 NuA4
histone acetyltransferase complex [PMID:10911987]"
/experiment="EXISTENCE:direct assay:GO:0042393 histone
binding [PMID:10911987]"
/experiment="EXISTENCE:mutant phenotype:GO:0006281 DNA
repair [PMID:15610740]"
/experiment="EXISTENCE:mutant phenotype:GO:0006325
chromatin organization [PMID:10436015]"
/experiment="EXISTENCE:mutant phenotype:GO:0006355
regulation of DNA-templated transcription [PMID:8598290]"
/experiment="EXISTENCE:mutant phenotype:GO:0051382
kinetochore assembly [PMID:17452364]"
/experiment="EXISTENCE:physical interaction:GO:0031011
Ino80 complex [PMID:24034245|PMID:10952318]"
/note="Nuclear actin-related protein involved in chromatin
remodeling; component of chromatin-remodeling enzyme
complexes"
/codon_start=1
/product="Arp4p"
/protein_id="NP_012454.1"
/db_xref="GeneID:853364"
/db_xref="SGD:S000003617"
/translation="MSNAALQVYGGDEVSAVVIDPGSYTTNIGYSGSDFPQSILPSVY
GKYTADEGNKKIFSEQSIGIPRKDYELKPIIENGLVIDWDTAQEQWQWALQNELYLNS
NSGIPALLTEPVWNSTENRKKSLEVLLEGMQFEACYLAPTSTCVSFAAGRPNCLVVDI
GHDTCSVSPIVDGMTLSKSTRRNFIAGKFINHLIKKALEPKEIIPLFAIKQRKPEFIK
KTFDYEVDKSLYDYANNRGFFQECKETLCHICPTKTLEETKTELSSTAKRSIESPWNE
EIVFDNETRYGFAEELFLPKEDDIPANWPRSNSGVVKTWRNDYVPLKRTKPSGVNKSD
KKVTPTEEKEQEAVSKSTSPAANSADTPNETGKRPLEEEKPPKENNELIGLADLVYSS
IMSSDVDLRATLAHNVVLTGGTSSIPGLSDRLMTELNKILPSLKFRILTTGHTIERQY
QSWLGGSILTSLGTFHQLWVGKKEYEEVGVERLLNDRFR"
gene complement(<285558..>289226)
/gene="SCP160"
/locus_tag="YJL080C"
/db_xref="GeneID:853365"
mRNA complement(<285558..>289226)
/gene="SCP160"
/locus_tag="YJL080C"
/product="Scp160p"
/transcript_id="NM_001181513.1"
/db_xref="GeneID:853365"
CDS complement(285558..289226)
/gene="SCP160"
/locus_tag="YJL080C"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:11278502]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640|PMID:22832273]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:11278502]"
/experiment="EXISTENCE:direct assay:GO:0042175 nuclear
outer membrane-endoplasmic reticulum membrane network
[PMID:8533468]"
/experiment="EXISTENCE:genetic interaction:GO:0000750
pheromone-dependent signal transduction involved in
conjugation with cellular fusion [PMID:14536090]"
/experiment="EXISTENCE:genetic interaction:GO:0031509
subtelomeric heterochromatin formation [PMID:16632467]"
/experiment="EXISTENCE:mutant phenotype:GO:0007059
chromosome segregation [PMID:8533468]"
/experiment="EXISTENCE:mutant phenotype:GO:0030466 silent
mating-type cassette heterochromatin formation
[PMID:16632467]"
/experiment="EXISTENCE:mutant phenotype:GO:0031509
subtelomeric heterochromatin formation [PMID:16632467]"
/experiment="EXISTENCE:mutant phenotype:GO:0043577
chemotropism [PMID:22832273]"
/experiment="EXISTENCE:mutant phenotype:GO:0045141 meiotic
telomere clustering [PMID:16632467]"
/experiment="EXISTENCE:physical interaction:GO:0001965
G-protein alpha-subunit binding [PMID:14536090]"
/note="Essential RNA-binding G protein effector of mating
response pathway; ligand-activated RNA-binding protein
that delivers RNAs involved in polarization and
perpetualizing mating signal to shmoo tip during pheromone
signaling; Scp160p-mediated RNA trafficking essential for
chemotropism and successful mating; mainly associated with
nuclear envelope and ER, interacts in mRNA-dependent
manner with translating ribosomes via multiple KH domains,
similar to vertebrate vigilins"
/codon_start=1
/product="Scp160p"
/protein_id="NP_012455.1"
/db_xref="GeneID:853365"
/db_xref="SGD:S000003616"
/translation="MSEEQTAIDSPPSTVEGSVETVTTIDSPSTTASTIAATAEEHPQ
LEKKPTPLPSLKDLPSLGSNAAFANVKVSWGPNMKPAVSNSPSPSPSAPSLTTGLGAK
RMRSKNIQEAFTLDLQSQLSITKPELSRIVQSVKKNHDVSVESTLSKNARTFLVSGVA
ANVHEAKRELVKKLTKPINAVIEVPSKCKASIIGSGGRTIREISDAYEVKINVSKEVN
ENSYDEDMDDTTSNVSLFGDFESVNLAKAKILAIVKEETKNATIKLVVEDEKYLPYID
VSEFASDEGDEEVKVQFYKKSGDIVILGPREKAKATKTSIQDYLKKLASNLDEEKVKI
PSKFQFLIDAEELKEKYNVIVTFPSTPDDELVSFVGLRDKVGEAITYARSSSKSYVVE
SLDISKAHSKNLTHAKNLIMYFTKYSVLKGLEESHPNVKISLPSIQSLPTAETVTIHI
SAKSDEANDIKAVRKELISFVNNIPPSETLVITDLDYELFGGSIKHCLLASESSVAFV
QFGDYYPNDNSILLVALTEDEDFKPSIEEIQASLNKANESLNSLRTKQNNMETKTYEF
SEEVQDSLFKPSSATWKLIMEDISEQEGHLQIKLHTPEENQLTVRGDEKAAKAANKIF
ESILNSPSSKSKMTVNIPANSVARLIGNKGSNLQQIREKFACQIDIPNEENNNASKDK
TVEVTLTGLEYNLTHAKKYLAAEAKKWADIITKELIVPVKFHGSLIGPHGTYRNRLQE
KYNVFINFPRDNEIVTIRGPSRGVNKAHEELKALLDFEMENGHKMVINVPAEHVPRII
GKNGDNINDIRAEYGVEMDFLQKSTDPKAQETGEVELEITGSRQNIKDAAKRVESIVA
EASDFVTEVLKIDHKYHKSIVGSGGHILREIISKAGGEEIRNKSVDIPNADSENKDIT
VQGPQKFVKKVVEEINKIVKDAENSVTKTIDIPAERKGALIGPGGIVRRQLESEFNIN
LFVPNKDDPSGKITITGAPENVEKAEKKILNEIIRENFDREVDVPASIYEYVSERGAF
IQKLRMDLSVNVRFGNTSKKANKLARAPIEIPLEKVCGSTEGENAEKTKFTIEEVGAP
TSSEEGDITMRLTYEPIDLSSILSDGEEKEVTKDTSNDSAKKEEALDTAVKLIKERIA
KAPSATYAGYVWGADTRRFNMIVGPGGSNIKKIREAADVIINVPRKSDKVNDVVYIRG
TKAGVEKAGEMVLKSLRR"
gene complement(<289875..>290774)
/gene="PRY1"
/locus_tag="YJL079C"
/db_xref="GeneID:853366"
mRNA complement(<289875..>290774)
/gene="PRY1"
/locus_tag="YJL079C"
/product="sterol-binding protein"
/transcript_id="NM_001181512.1"
/db_xref="GeneID:853366"
CDS complement(289875..290774)
/gene="PRY1"
/locus_tag="YJL079C"
/experiment="EXISTENCE:direct assay:GO:0000287 magnesium
ion binding [PMID:27344972]"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005504 fatty acid
binding [PMID:28365570]"
/experiment="EXISTENCE:direct assay:GO:0005576
extracellular region [PMID:11935221]"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0032934 sterol
binding [PMID:23027975]"
/experiment="EXISTENCE:genetic interaction:GO:0015908
fatty acid transport [PMID:28365570]"
/experiment="EXISTENCE:genetic interaction:GO:0015918
sterol transport [PMID:23027975]"
/experiment="EXISTENCE:mutant phenotype:GO:0005504 fatty
acid binding [PMID:28365570]"
/experiment="EXISTENCE:mutant phenotype:GO:0015918 sterol
transport [PMID:23027975]"
/experiment="EXISTENCE:mutant phenotype:GO:0032934 sterol
binding [PMID:23027975]"
/note="Lipid binding protein; binds to both free fatty
acids with preference for saturated long-chain fatty acids
and to sterols; contains two independent lipid-binding
sites; involved in the export of free fatty acids and
acetylated sterols; export functions are redundant with
PRY2, and PRY3; important for survival of cells that
accumulate free fatty acids; involved in detoxification of
hydrophobic compounds; secreted glycoprotein and member of
the CAP/SCP protein superfamily"
/codon_start=1
/product="sterol-binding protein"
/protein_id="NP_012456.1"
/db_xref="GeneID:853366"
/db_xref="SGD:S000003615"
/translation="MKLSKLSILTSALATSALAAPAVVTVTEHAHEAAVVTVQGVVYV
ENGQTRTTYETLAPASTATPTSTATALVAPPVAPSSASSNSDVVLSALKNLASVWGKT
TDSTTTLTSSESTSQSLAQATTTSTPAAASTTSTPAATTTTSQAAATSSASSSDSDLS
DFASSVLAEHNKKRALHKDTPALSWSDTLASYAQDYADNYDCSGTLTHSGGPYGENLA
LGYDGPAAVDAWYNEISNYDFSNPGFSSNTGHFTQVVWKSTTQVGCGIKTCGGAWGDY
VICSYDPAGNYEGEYADNVEPLA"
gene complement(<291336..>293981)
/gene="PRY3"
/locus_tag="YJL078C"
/db_xref="GeneID:853367"
mRNA complement(<291336..>293981)
/gene="PRY3"
/locus_tag="YJL078C"
/product="Pry3p"
/transcript_id="NM_001181511.1"
/db_xref="GeneID:853367"
CDS complement(291336..293981)
/gene="PRY3"
/locus_tag="YJL078C"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:32554483|PMID:10383953|PMID:15781460]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0015908
fatty acid transport [PMID:28365570]"
/experiment="EXISTENCE:mutant phenotype:GO:0015918 sterol
transport [PMID:23027975]"
/note="GPI-anchored cell wall protein involved in export
of sterols; redundant role with PRY1 and PRY2 in the
export of free fatty acids; role in mating efficiency;
expression of full-length transcript is daughter
cell-specific; in response to alpha factor, a short
transcript starting at +452 is expressed and the long form
is repressed by Ste12p; member of the CAP protein
superfamily (cysteine-rich secretory proteins (CRISP),
antigen 5, and pathogenesis related 1 proteins)"
/codon_start=1
/product="Pry3p"
/protein_id="NP_012457.1"
/db_xref="GeneID:853367"
/db_xref="SGD:S000003614"
/translation="MLEFPISVLLGCLVAVKAQTTFPNFESDVLNEHNKFRALHVDTA
PLTWSDTLATYAQNYADQYDCSGVLTHSDGPYGENLALGYTDTGAVDAWYGEISKYNY
SNPGFSESTGHFTQVVWKSTAEIGCGYKYCGTTWNNYIVCSYNPPGNYLGEFAEEVEP
LISTVSSSSSSSSSTSTTSDTVSTISSSIMPAVAQGYTTTVSSAASSSSLKSTTINPA
KTATLTASSSTVITSSTESVGSSTVSSASSSSVTTSYATSSSTVVSSDATSSTTTTSS
VATSSSTTSSDPTSSTAAASSSDPASSSAAASSSASTENAASSSSAISSSSSMVSAPL
SSTLTTSTASSRSVTSNSVNSVKFANTTVFSAQTTSSVSASLSSSVAADDIQGSTSKE
ATSSVSEHTSIVTSATNAAQYATRLGSSSRSSSGAVSSSAVSQSVLNSVIAVNTDVSV
TSVSSTAHTTKDTATTSVTASESITSETAQASSSTEKNISNSAATSSSIYSNSASVSG
HGVTYAAEYAITSEQSSALATSVPATNCSSIVKTTTLENSSTTTITAITKSTTTLATT
ANNSTRAATAVTIDPTLDPTDNSASPTDNAKHTSTYGSSSTGASLDSLRTTTSISVSS
NTTQLVSTCTSESDYSDSPSFAISTATTTESNLITNTITASCSTDSNFPTSAASSTDE
TAFTRTISTSCSTLNGASTQTSELTTSPMKTNTVVPASSFPSTTTTCLENDDTAFSSI
YTEVNAATIINPGETSSLASDFATSEKPNEPTSVKSTSNEGTSSTTTTYQQTVATLYA
KPSSTSLGARTTTGSNGRSTTSQQDGSAMHQPTSSIYTQLKEGTSTTAKLSAYEGAAT
PLSIFQCNSLAGTIAAFVVAVLFAF"
gene <294047..>294145
/locus_tag="YJL077W-B"
/db_xref="GeneID:1466467"
mRNA <294047..>294145
/locus_tag="YJL077W-B"
/product="uncharacterized protein"
/transcript_id="NM_001184603.1"
/db_xref="GeneID:1466467"
CDS 294047..294145
/locus_tag="YJL077W-B"
/note="hypothetical protein; identified by gene-trapping,
microarray-based expression analysis, and genome-wide
homology searching"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878104.1"
/db_xref="GeneID:1466467"
/db_xref="SGD:S000028662"
/translation="MPILVWQLPKSKKLKTLKYRICFREFFDRVET"
gene complement(<294666..>295061)
/gene="ICS3"
/locus_tag="YJL077C"
/db_xref="GeneID:853368"
mRNA complement(<294666..>295061)
/gene="ICS3"
/locus_tag="YJL077C"
/product="Ics3p"
/transcript_id="NM_001181510.1"
/db_xref="GeneID:853368"
CDS complement(294666..295061)
/gene="ICS3"
/locus_tag="YJL077C"
/experiment="EXISTENCE:mutant phenotype:GO:0055070 copper
ion homeostasis [PMID:26127016]"
/note="Protein with a role in copper homeostasis; possible
role in vacuolar sorting and processing of secretory
proteins; null mutants are hypersensitive to sortin2"
/codon_start=1
/product="Ics3p"
/protein_id="NP_012458.1"
/db_xref="GeneID:853368"
/db_xref="SGD:S000003613"
/translation="MHISLPTRNKSYFRIRTRTYQIGLYHSDSSPIRDISVLHLLIAT
LCTIFFPIFFSLSKVQVVQWQGTTISKNCIALTMSFPLNAIPGMYLIIAFPRLQTVIP
LQRNTPVRITKSVIVKGAVSVPRISSPMH"
gene <294799..>294885
/locus_tag="YJL077W-A"
/db_xref="GeneID:13393612"
mRNA <294799..>294885
/locus_tag="YJL077W-A"
/product="uncharacterized protein"
/transcript_id="NM_001270753.1"
/db_xref="GeneID:13393612"
CDS 294799..294885
/locus_tag="YJL077W-A"
/note="hypothetical protein; mRNA identified as translated
by ribosome profiling data; completely overlaps the
verified gene YJL077C/ICS3"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001257682.1"
/db_xref="GeneID:13393612"
/db_xref="SGD:S000028661"
/translation="MPGIAFKGKDMVKAIQFLEIVVPCHCTT"
gene <295245..>298814
/gene="NET1"
/locus_tag="YJL076W"
/gene_synonym="CFI1; ESC5; SRM8"
/db_xref="GeneID:853369"
mRNA <295245..>298814
/gene="NET1"
/locus_tag="YJL076W"
/gene_synonym="CFI1; ESC5; SRM8"
/product="Net1p"
/transcript_id="NM_001181509.1"
/db_xref="GeneID:853369"
CDS 295245..298814
/gene="NET1"
/locus_tag="YJL076W"
/gene_synonym="CFI1; ESC5; SRM8"
/experiment="EXISTENCE:direct assay:GO:0000182 rDNA
binding [PMID:10219245]"
/experiment="EXISTENCE:direct assay:GO:0004864 protein
phosphatase inhibitor activity [PMID:10219244]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:10219244]"
/experiment="EXISTENCE:direct assay:GO:0030869 RENT
complex [PMID:10219244]"
/experiment="EXISTENCE:direct assay:GO:1902570 protein
localization to nucleolus [PMID:33481703]"
/experiment="EXISTENCE:genetic interaction:GO:0001100
negative regulation of exit from mitosis [PMID:10219244]"
/experiment="EXISTENCE:mutant phenotype:GO:0000183 rDNA
heterochromatin formation [PMID:10219245]"
/experiment="EXISTENCE:mutant phenotype:GO:0001100
negative regulation of exit from mitosis
[PMID:10219244|PMID:26090959]"
/experiment="EXISTENCE:mutant phenotype:GO:0007000
nucleolus organization [PMID:10219245]"
/experiment="EXISTENCE:mutant phenotype:GO:1904751
positive regulation of protein localization to nucleolus
[PMID:10219244]"
/note="Core subunit of the RENT complex; involved in
nucleolar silencing and telophase exit; stimulates
transcription by RNA polymerase I and regulates nucleolar
structure; NET1 has a paralog, TOF2, that arose from the
whole genome duplication"
/codon_start=1
/product="Net1p"
/protein_id="NP_012459.1"
/db_xref="GeneID:853369"
/db_xref="SGD:S000003612"
/translation="MYKLQVVLVPPSLQATMPIQFGYGPTIAESSQLLPNRTNMAQSA
GDASLQYANLRSANVSFTPSYFNQSRFRKFLLFTKPTNTLLNLSDEIIDKCEKMYPSL
QEDIEILSLQDNSGCDLDPDFLVKDVFNVNNIVRVILKNEIDLDDSAPVSLYKSVKRS
KLNNGSPQSVQPQQQIPSSSGVLRIAKKRPPTGTTTTTTIRSATNGSMRVSTPLARQI
YPPPSSKIVSNNSDDEDEDIGERSFLPPPTQPQSPPIRISSGIDAGKKIKSSIVEEDI
VSRSATVDPDKTKQQRLLSGTPIMSTMTPNRVTLTGQRVVSEHAHKNELVFSASASSS
SFANGGTAAVTAQDINRKPPVTTPRITSGMLKIPEPRISEIEKELKEGPSSPASILPA
KAAKIPMKKPYLENGENYESDDSSSSENQETPETEPHSKASLQRSQSSIADNNGSPVK
NSPLGDAMPHNVHLAELPKASNTSITKSSNGESWGKQQEHQPPRKSSLETIVEKKSQA
EPSGIVEPKRMTNFLDDNQVREKEDTNDKLLEKEILPTIPHNDQPILASSDKSNGTLK
SLAGKVSSNNNASKEDGTIINGTIEDDGNDNDEVDTTVRIVPQDSDSSSFPKSDLFKM
IEGDDTDLPQWFKGKNSRTSGNSKNSKPYTTVLNKDIDNSKPDPRNILPQRTPRSAAK
RAAQLLAGAKKNEVPQKSTEDSSSAASTDDESESGIETDFSSDDDFKRKNMSVPNNGP
KDISLHSLKGSVVPVKDSKIINKEVDEERNDKRDSQKKSAVSESSVTNSKISEQMAKS
FYPNSNKKQNEATKVETKPATQASSFPVVGGSPSVATKGTTSFNEEGNRKNVKTKAKN
ESAQIDRQQKETTSRVADLKSANIGGEDLNKKAEGSKEPEKASANIQDANDKNNSKEK
EDSKSKQVSQKKLKMTDHLKEGNVQLPKPSANDKLKDLKAKFTNSKTLVPPGIISNEK
NNSSANDDDSSSSGSSTEDESSSSSSSSDEETSTSRKARRVVVNTPREPVRSSSKIEA
PSPSVNKKINATPDKIPVTQLMDMSSPPSVKSKTTSNPSSILHDLPRKVRPSLSSLSD
LVSRGIPDVKEKTSKSNEKSQTKAPSSSDDESSSDSDSNSSSDSVSDSSSDSKSESDS
DDSGDSSDDGKSFISAKSASAALGKKKKPSGGFASLIKDFKKK"
gene complement(<298460..>298876)
/gene="APQ13"
/locus_tag="YJL075C"
/db_xref="GeneID:853370"
mRNA complement(<298460..>298876)
/gene="APQ13"
/locus_tag="YJL075C"
/product="Apq13p"
/transcript_id="NM_001395035.1"
/db_xref="GeneID:853370"
CDS complement(298460..298876)
/gene="APQ13"
/locus_tag="YJL075C"
/note="hypothetical protein; not conserved in closely
related Saccharomyces species; 85% of ORF overlaps
verified gene NET1, but does not share all phenotypes;
null mutant is slow-growing, sensitive to heat, sorbate,
and other various chemicals"
/codon_start=1
/product="Apq13p"
/protein_id="NP_001381965.1"
/db_xref="GeneID:853370"
/db_xref="SGD:S000003611"
/translation="MLDYFFLLAFCDVYSTETFWYHFFLKSFINDANPPLGFFFLPKA
ALADFALIKLFPSSDESPESSESDSDLESELESDTESELELESESELDSSSLLEGAFV
CDFSFDLEVFSFTSGMPLETRSDNELKEGRTFLGRS"
rep_origin 298981..299245
/note="ARS1010; Autonomously Replicating Sequence"
/db_xref="SGD:S000028447"
gene complement(<299157..>302849)
/gene="SMC3"
/locus_tag="YJL074C"
/db_xref="GeneID:853371"
mRNA complement(<299157..>302849)
/gene="SMC3"
/locus_tag="YJL074C"
/product="cohesin subunit SMC3"
/transcript_id="NM_001181507.1"
/db_xref="GeneID:853371"
CDS complement(299157..302849)
/gene="SMC3"
/locus_tag="YJL074C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:15282802]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:17055978]"
/experiment="EXISTENCE:direct assay:GO:0030892 mitotic
cohesin complex [PMID:9990856]"
/experiment="EXISTENCE:genetic interaction:GO:0007064
mitotic sister chromatid cohesion [PMID:9335333]"
/experiment="EXISTENCE:mutant phenotype:GO:0007130
synaptonemal complex assembly [PMID:10412984]"
/experiment="EXISTENCE:mutant phenotype:GO:0007131
reciprocal meiotic recombination [PMID:10412984]"
/experiment="EXISTENCE:mutant phenotype:GO:0051177 meiotic
sister chromatid cohesion [PMID:10412984]"
/experiment="EXISTENCE:mutant phenotype:GO:1990414
replication-born double-strand break repair via sister
chromatid exchange [PMID:16888651]"
/experiment="EXISTENCE:physical interaction:GO:0000086
G2/M transition of mitotic cell cycle [PMID:27325700]"
/experiment="EXISTENCE:physical interaction:GO:0019901
protein kinase binding [PMID:27325700]"
/note="Subunit of the multiprotein cohesin complex;
required for sister chromatid cohesion in mitotic cells;
also required, with Rec8p, for cohesion and recombination
during meiosis; phylogenetically conserved SMC chromosomal
ATPase family member"
/codon_start=1
/product="cohesin subunit SMC3"
/protein_id="NP_012461.1"
/db_xref="GeneID:853371"
/db_xref="SGD:S000003610"
/translation="MYIKRVIIKGFKTYRNETIIDNFSPHQNVIIGSNGSGKSNFFAA
IRFVLSDDYSNLKREERQGLIHQGSGGSVMSASVEIVFHDPDHSMILPSGVLSRGDDE
VTIRRTVGLKKDDYQLNDRNVTKGDIVRMLETAGFSMNNPYNIVPQGKIVALTNAKDK
ERLQLLEDVVGAKSFEVKLKASLKKMEETEQKKIQINKEMGELNSKLSEMEQERKELE
KYNELERNRKIYQFTLYDRELNEVINQMERLDGDYNNTVYSSEQYIQELDKREDMIDQ
VSKKLSSIEASLKIKNATDLQQAKLRESEISQKLTNVNVKIKDVQQQIESNEEQRNLD
SATLKEIKSIIEQRKQKLSKILPRYQELTKEEAMYKLQLASLQQKQRDLILKKGEYAR
FKSKDERDTWIHSEIEELKSSIQNLNELESQLQMDRTSLRKQYSAIDEEIEELIDSIN
GPDTKGQLEDFDSELIHLKQKLSESLDTRKELWRKEQKLQTVLETLLSDVNQNQRNVN
ETMSRSLANGIINVKEITEKLKISPESVFGTLGELIKVNDKYKTCAEVIGGNSLFHIV
VDTEETATLIMNELYRMKGGRVTFIPLNRLSLDSDVKFPSNTTTQIQFTPLIKKIKYE
PRFEKAVKHVFGKTIVVKDLGQGLKLAKKHKLNAITLDGDRADKRGVLTGGYLDQHKR
TRLESLKNLNESRSQHKKILEELDFVRNELNDIDTKIDQVNGNIRKVSNDRESVLTNI
EVYRTSLNTKKNEKLILEESLNAIILKLEKLNTNRTFAQEKLNTFENDLLQEFDSELS
KEEKERLESLTKEISAAHNKLNITSDALEGITTTIDSLNAELESKLIPQENDLESKMS
EVGDAFIFGLQDELKELQLEKESVEKQHENAVLELGTVQREIESLIAEETNNKKLLEK
ANNQQRLLLKKLDNFQKSVEKTMIKKTTLVTRREELQQRIREIGLLPEDALVNDFSDI
TSDQLLQRLNDMNTEISGLKNVNKRAFENFKKFNERRKDLAERASELDESKDSIQDLI
VKLKQQKVNAVDSTFQKVSENFEAVFERLVPRGTAKLIIHRKNDNANDHDESIDVDMD
AESNESQNGKDSEIMYTGVSISVSFNSKQNEQLHVEQLSGGQKTVCAIALILAIQMVD
PASFYLFDEIDAALDKQYRTAVATLLKELSKNAQFICTTFRTDMLQVADKFFRVKYEN
KISTVIEVNREEAIGFIRGSNKFAEV"
gene <303181..>305118
/gene="JEM1"
/locus_tag="YJL073W"
/gene_synonym="KAR8"
/db_xref="GeneID:853372"
mRNA <303181..>305118
/gene="JEM1"
/locus_tag="YJL073W"
/gene_synonym="KAR8"
/product="Jem1p"
/transcript_id="NM_001181506.3"
/db_xref="GeneID:853372"
CDS 303181..305118
/gene="JEM1"
/locus_tag="YJL073W"
/gene_synonym="KAR8"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762|PMID:9148890]"
/experiment="EXISTENCE:direct assay:GO:0042175 nuclear
outer membrane-endoplasmic reticulum membrane network
[PMID:15282802]"
/experiment="EXISTENCE:genetic interaction:GO:0034975
protein folding in endoplasmic reticulum [PMID:9817751]"
/experiment="EXISTENCE:genetic interaction:GO:0051087
protein-folding chaperone binding [PMID:9148890]"
/experiment="EXISTENCE:mutant phenotype:GO:0000742
karyogamy involved in conjugation with cellular fusion
[PMID:10069807]"
/experiment="EXISTENCE:mutant phenotype:GO:0036503 ERAD
pathway [PMID:11381090]"
/experiment="EXISTENCE:mutant phenotype:GO:0051082
unfolded protein binding [PMID:9148890]"
/note="DnaJ-like chaperone required for nuclear membrane
fusion during mating; localizes to the ER membrane;
exhibits genetic interactions with KAR2"
/codon_start=1
/product="Jem1p"
/protein_id="NP_012462.3"
/db_xref="GeneID:853372"
/db_xref="SGD:S000003609"
/translation="MILISGYCLLVYSVILPVLISASKLCDLAELQRLNKNLKVDTES
LPKYQWIAGQLEQNCMTADPASENMSDVIQLANQIYYKIGLIQLSNDQHLRAINTFEK
IVFNETYKGSFGKLAEKRLQELYVDFGMWDKVHQKDDQYAKYLSLNETIRNKISSKDV
SVEEDISELLRITPYDVNVLSTHIDVLFHKLAEEIDVSLAAAIILDYETILDKHLASL
SIDTRLSIHYVISVLQTFVLNSDASFNIRKCLSIDMDYDKCKKLSLTISKLNKVNPSK
RQILDPATYAFENKKFRSWDRIIEFYLKDKKPFITPMKILNKDTNFKNNYFFLEEIIK
QLIEDVQLSRPLAKNLFEDPPITDGFVKPKSYYHTDYLVYIDSILCQASSMSPDVKRA
KLAAPFCKKSLRHSLTLETWKHYQDAKSEQKPLPETVLSDVWNSNPHLLMYMVNSILN
KSRSKPHSQFKKQLYDQINKFFQDNGLSESTNPYVMKNFRLLQKQLQTYKEHKHRNFN
QQYFQQQQQQQQHQRHQAPPAAPNYDPKKDYYKILGVSPSASSKEIRKAYLNLTKKYH
PDKIKANHNDKQESIHETMSQINEAYETLSDDDKRKEYDLSRSNPRRNTFPQGPRQNN
MFKNPGSGFPFGNGFKMNFGL"
gene complement(<305221..>305862)
/gene="PSF2"
/locus_tag="YJL072C"
/gene_synonym="CDC102"
/db_xref="GeneID:853373"
mRNA complement(<305221..>305862)
/gene="PSF2"
/locus_tag="YJL072C"
/gene_synonym="CDC102"
/product="DNA replication protein PSF2"
/transcript_id="NM_001181505.1"
/db_xref="GeneID:853373"
CDS complement(305221..305862)
/gene="PSF2"
/locus_tag="YJL072C"
/gene_synonym="CDC102"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:15282802]"
/experiment="EXISTENCE:direct assay:GO:0043596 nuclear
replication fork [PMID:16103218]"
/experiment="EXISTENCE:direct assay:GO:0071162 CMG complex
[PMID:26524492]"
/experiment="EXISTENCE:genetic interaction:GO:0006261
DNA-templated DNA replication [PMID:12730134]"
/experiment="EXISTENCE:mutant phenotype:GO:0000727
double-strand break repair via break-induced replication
[PMID:20516198]"
/experiment="EXISTENCE:mutant phenotype:GO:0006261
DNA-templated DNA replication [PMID:12768207]"
/experiment="EXISTENCE:physical interaction:GO:0000811
GINS complex [PMID:12730134|PMID:12768207]"
/experiment="EXISTENCE:physical interaction:GO:0031261 DNA
replication preinitiation complex [PMID:12730134]"
/note="Subunit of the GINS complex (Sld5p, Psf1p, Psf2p,
Psf3p); complex is localized to DNA replication origins
and implicated in assembly of the DNA replication
machinery"
/codon_start=1
/product="DNA replication protein PSF2"
/protein_id="NP_012463.1"
/db_xref="GeneID:853373"
/db_xref="SGD:S000003608"
/translation="MSLPAHLQQTFSPEEIQFIVENEPIKIFPRITTRQKIRGDDRGT
GNHTRWQLITTDDKALNNMVAMRSTEVVLWIALLLKQQSKCSIVAPQWLTTKELDRKI
QYEKTHPDRFSELPWNWLVLARILFNKAKDDFHDPIHELRGKIQDLREIRQIKVLKGL
KYLNESHLQLDNLSLLEINELRPFITEIMDKLREIHTASLTAGTENDEEEFNI"
gene <306132..>307856
/gene="ARG2"
/locus_tag="YJL071W"
/gene_synonym="HRB574"
/db_xref="GeneID:853374"
mRNA <306132..>307856
/gene="ARG2"
/locus_tag="YJL071W"
/gene_synonym="HRB574"
/product="acetyl-CoA:L-glutamate N-acetyltransferase"
/transcript_id="NM_001181504.1"
/db_xref="GeneID:853374"
CDS 306132..307856
/gene="ARG2"
/locus_tag="YJL071W"
/gene_synonym="HRB574"
/EC_number="2.3.1.1"
/experiment="EXISTENCE:direct assay:GO:0004042 L-glutamate
N-acetyltransferase activity [PMID:11553611]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:205532]"
/experiment="EXISTENCE:mutant phenotype:GO:0004042
L-glutamate N-acetyltransferase activity [PMID:11553611]"
/experiment="EXISTENCE:mutant phenotype:GO:0006592
ornithine biosynthetic process [PMID:11553611]"
/note="Acetylglutamate synthase (glutamate
N-acetyltransferase); mitochondrial enzyme that catalyzes
the first step in the biosynthesis of the arginine
precursor ornithine; forms a complex with Arg5,6p"
/codon_start=1
/product="acetyl-CoA:L-glutamate N-acetyltransferase"
/protein_id="NP_012464.1"
/db_xref="GeneID:853374"
/db_xref="SGD:S000003607"
/translation="MWRRIFAHELKYDQPNASSKNLILSVLNTTATKREAKDYLSKYT
NDSGQHNHCLFFIRDLHKVAPAILSQFSSVIKRLGMLGLRPMFVIPPSPTHVNIQAEL
LDSIVTEADLKPLHLKEGLTKSRTGLYHSVFSQESRFFDIGNSNFIPIVKPYVYNEET
ASEFMTKDVVKFMDCLCQGNIPHIDKFFILNNAGGIPSGERNDNAHVFINLSQELEHL
SSSLSHNISTLTKREPRSQNLLHRMEVYVKKDEISSLECEYHDHLENLLLMDKVLSNL
AATATGLITTVKAAALSSDRKNPLVYNLLTDRSLISSSLPRFKKKDGEIDSPANMFDD
HAWYELPSQQVNAAPSNSDAVLVTTVLKKGVHIKTYDYKTLTQFNSIGLPKKFHVPEK
GAKPSSNSPKLDINKFKSIIDQSFKRSLDLHDYIKRINGKIATIIVIGDYEGIAILTY
EGSEENSFVYLDKFAVLPHLKGSLGISDIIFNLMFKKFPNEILWRSRKDNVVNKWYFQ
RSVAVLDLSIDLDPEHCDEKQSQFKLFYYGNPQYAKRALRDKKRLREFMRSVRDIKPS
WENEKNIS"
gene complement(<307971..>310637)
/locus_tag="YJL070C"
/db_xref="GeneID:853375"
mRNA complement(<307971..>310637)
/locus_tag="YJL070C"
/product="metallo-dependent hydrolase superfamily protein"
/transcript_id="NM_001181503.3"
/db_xref="GeneID:853375"
CDS complement(307971..310637)
/locus_tag="YJL070C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/note="Putative metallo-dependent hydrolase superfamily
protein; similar to AMP deaminases but lacks key catalytic
residues and does not rescue purine nucleotide metabolic
defect of quadruple aah1 ade8 amd1 his1 mutant; may
regulate purine nucleotide homeostasis as overexpression
in an AMD1 strain grown in adenine results in greatly
reduced GDP and GTP intracellular levels; not an essential
gene; YJL070C has a paralog, YBR284W, that arose from the
whole genome duplication"
/codon_start=1
/product="metallo-dependent hydrolase superfamily protein"
/protein_id="NP_012465.3"
/db_xref="GeneID:853375"
/db_xref="SGD:S000003606"
/translation="MQAVERRPSLLFDEYQNSVTKPNETKNKEARVLSENDGDVSPSV
LKQKEISVDDMDMISLPTEFDRQMVLGSPMFFDLEDEENKIDPLPSVSHHYGNGESDS
FVSSYTPSNLKTGEETKDLFINPFELVSQMRKRYIAASKQDGISNIKNDTEKWFLYPK
PLPKFWRFEDDKRFQDPSDSDLNDDGDSTGTGAATPHRHGYYYPSYFTDHYYYYTKSG
LKGKGNIKVPYTGEYFDLEDYKKQYIYHLSNQENTQNPLSPYSSKEESLEEEFLTDVP
TFQEFRDDFAYIIELIQSHKFNEVSRKRLSYLLDKFELFQYLNSKKEILANKNVPYRD
FYNSRKVDRDLSLSGCISQRQLSEYIWEKINLEPERIVYQDPETSRKLSLRDIFQFGC
SSNDQPIAIGLKLIDDEFLDWYRNIYLIDYHLTPNKVAKLVGKEMRFYLLAKVFLEFD
NFIEGEYLAEIFIKYVIHILEKSKYQLAQVSVNFQFYSSGEDWYKKFSQWLLRWKLVS
YNIRWNIQIARIFPKLFKENVVSNFQEFLDLIFNPLFTLEKEQLPIDSSVNTDIIGLQ
FFLSNVCSMDLVIKESDEYYWKEFTDMNCKPKFWTAQGDNPTVAHYMYYIYKSLAKVN
FLRSQNLQNTITLRNYCSPLSSRTSQFGVDLYFTDQVESLVCNLLLCNGGLLQVEPLW
DTATMIQYLFYLFQIPILAAPLSSVSLLNSQKSTFLKNKNVLLEHDYLKDQETAKINP
SRDITVGEQRSYETNPFMKMFKMGLKISLSSKSILYNSSYTLEPLIEEYSVAASIYLL
NPTDLCELSRTSVLSSGYEGWYKAHWIGVGVKKAPYFEENVGGIDNWYDTAKDTSIKH
NVPMIRRRYRKETLDQEWNFVRDHFGVINSIW"
gene complement(<310922..>312706)
/gene="UTP18"
/locus_tag="YJL069C"
/db_xref="GeneID:853376"
mRNA complement(<310922..>312706)
/gene="UTP18"
/locus_tag="YJL069C"
/product="Utp18p"
/transcript_id="NM_001181502.1"
/db_xref="GeneID:853376"
CDS complement(310922..312706)
/gene="UTP18"
/locus_tag="YJL069C"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:15590835|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/experiment="EXISTENCE:direct assay:GO:0032040
small-subunit processome [PMID:15590835]"
/experiment="EXISTENCE:direct assay:GO:0034388
Pwp2p-containing subcomplex of 90S preribosome
[PMID:15231838|PMID:17515605]"
/experiment="EXISTENCE:mutant phenotype:GO:0000447
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from
5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15590835]"
/experiment="EXISTENCE:mutant phenotype:GO:0000472
endonucleolytic cleavage to generate mature 5'-end of
SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:15590835]"
/experiment="EXISTENCE:mutant phenotype:GO:0000480
endonucleolytic cleavage in 5'-ETS of tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:15590835]"
/note="Small-subunit processome protein involved in
pre-18S rRNA maturation; part of a subunit of the 90S
preribosomal particle capable of interacting directly with
the 5' ETS of the 35S pre-rRNA; contains WD40 repeats"
/codon_start=1
/product="Utp18p"
/protein_id="NP_012466.1"
/db_xref="GeneID:853376"
/db_xref="SGD:S000003605"
/translation="MTMATTAMNVSVPPPDEEEQLLAKFVFGDTTDLQENLAKFNADF
IFNEQEMDVEDQEDEGSESDNSEEDEAQNGELDHVNNDQLFFVDDGGNEDSQDKNEDT
MDVDDEDDSSSDDYSEDSEEAAWIDSDDEKIKVPILVTNKTKKLRTSYNESKINGVHY
INRLRSQFEKIYPRPKWVDDESDSELDDEEDDEEEGSNNVINGDINALTKILSTTYNY
KDTLSNSKLLPPKKLDIVRLKDANASHPSHSAIQSLSFHPSKPLLLTGGYDKTLRIYH
IDGKTNHLVTSLHLVGSPIQTCTFYTSLSNQNQQNIFTAGRRRYMHSWDLSLENLTHS
QTAKIEKFSRLYGHESTQRSFENFKVAHLQNSQTNSVHGIVLLQGNNGWINILHSTSG
LWLMGCKIEGVITDFCIDYQPISRGKFRTILIAVNAYGEVWEFDLNKNGHVIRRWKDQ
GGVGITKIQVGGGTTTTCPALQISKIKQNRWLAVGSESGFVNLYDRNNAMTSSTPTPV
AALDQLTTTISNLQFSPDGQILCMASRAVKDALRLVHLPSCSVFSNWPTSGTPLGKVT
SVAFSPSGGLLAVGNEQGKVRLWKLNHY"
gene complement(<313016..>313915)
/locus_tag="YJL068C"
/db_xref="GeneID:853377"
mRNA complement(<313016..>313915)
/locus_tag="YJL068C"
/product="S-formylglutathione hydrolase"
/transcript_id="NM_001181501.1"
/db_xref="GeneID:853377"
CDS complement(313016..313915)
/locus_tag="YJL068C"
/EC_number="3.1.2.12"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:10427036]"
/experiment="EXISTENCE:direct assay:GO:0018738
S-formylglutathione hydrolase activity [PMID:10427036]"
/experiment="EXISTENCE:mutant phenotype:GO:0046294
formaldehyde catabolic process [PMID:10427036]"
/note="Esterase that can function as an
S-formylglutathione hydrolase; non-essential intracellular
esterase; may be involved in the detoxification of
formaldehyde, which can be metabolized to
S-formylglutathione; similar to human esterase D"
/codon_start=1
/product="S-formylglutathione hydrolase"
/protein_id="NP_012467.1"
/db_xref="GeneID:853377"
/db_xref="SGD:S000003604"
/translation="MKVVKEFSVCGGRLIKLSHNSNSTKTSMNVNIYLPKHYYAQDFP
RNKRIPTVFYLSGLTCTPDNASEKAFWQFQADKYGFAIVFPDTSPRGDEVANDPEGSW
DFGQGAGFYLNATQEPYAQHYQMYDYIHKELPQTLDSHFNKNGDVKLDFLDNVAITGH
SMGGYGAICGYLKGYSGKRYKSCSAFAPIVNPSNVPWGQKAFKGYLGEEKAQWEAYDP
CLLIKNIRHVGDDRILIHVGDSDPFLEEHLKPELLLEAVKATSWQDYVEIKKVHGFDH
SYYFVSTFVPEHAEFHARNLGLI"
gene complement(<314114..>314872)
/gene="MPM1"
/locus_tag="YJL066C"
/db_xref="GeneID:853379"
mRNA complement(<314114..>314872)
/gene="MPM1"
/locus_tag="YJL066C"
/product="Mpm1p"
/transcript_id="NM_001181499.3"
/db_xref="GeneID:853379"
CDS complement(314114..314872)
/gene="MPM1"
/locus_tag="YJL066C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:11791739|PMID:24769239|PMID:16823961|PMID:14576278|P
MID:9874242]"
/experiment="EXISTENCE:direct assay:GO:0005758
mitochondrial intermembrane space
[PMID:22984289|PMID:22993211]"
/note="Mitochondrial intermembrane space hypothetical
protein"
/codon_start=1
/product="Mpm1p"
/protein_id="NP_012469.3"
/db_xref="GeneID:853379"
/db_xref="SGD:S000003602"
/translation="MGFYEGDDNDANTKAFNDKYIKDQKFATAPFWNLFPKLRDIDEY
DNPLLPLPFNFNFRDLGDSALAMASGIPTVKQFDKCEELKGQSAWTTQGIWKCLVPSK
AIPPLPQLDFLLPLEEIKSDKSHSHGLFFNDFNLFLKWRSHMNRLQKQRIKTRSTAVE
PLARTPEDLMLNWDDLHLGNDAEYASADGSKKIVGRAQSISTTKDSNDAKPSTVKTEK
IYFDDGTVDITTTTTSKGSSPQVKHKVVSVDEDN"
gene complement(<315054..>315557)
/gene="DLS1"
/locus_tag="YJL065C"
/db_xref="GeneID:853381"
mRNA complement(<315054..>315557)
/gene="DLS1"
/locus_tag="YJL065C"
/product="Dls1p"
/transcript_id="NM_001181498.1"
/db_xref="GeneID:853381"
CDS complement(315054..315557)
/gene="DLS1"
/locus_tag="YJL065C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008623 CHRAC
[PMID:15024052]"
/experiment="EXISTENCE:mutant phenotype:GO:0031509
subtelomeric heterochromatin formation [PMID:14673157]"
/experiment="EXISTENCE:mutant phenotype:GO:1903775
regulation of DNA double-strand break processing
[PMID:34362907]"
/note="Subunit of ISW2/yCHRAC chromatin accessibility
complex; ISW2/yCHRAC also includes Itc1p, Isw2p, and
Dpb4p; involved in inheritance of telomeric silencing;
DLS1 has a paralog, DPB3, that arose from the whole genome
duplication"
/codon_start=1
/product="Dls1p"
/protein_id="NP_012470.1"
/db_xref="GeneID:853381"
/db_xref="SGD:S000003601"
/translation="MNNETSGKETASAPLCSPKLPVEKVQRIAKNDPEYMDTSDDAFV
ATAFATEFFVQVLTHESLHRQQQQQQQQVPPLPDELTLSYDDISAAIVHSSDGHLQFL
NDVIPTTKNLRLLVEENRVRYTTSVMPPNEVYSAYVVNDTAPKPNIVEIDLDNDEDDD
EDVTDQE"
gene complement(<315759..>316475)
/gene="MRPL8"
/locus_tag="YJL063C"
/gene_synonym="bL17m; HRD238"
/db_xref="GeneID:853382"
mRNA complement(<315759..>316475)
/gene="MRPL8"
/locus_tag="YJL063C"
/gene_synonym="bL17m; HRD238"
/product="mitochondrial 54S ribosomal protein bL17m MRPL8"
/transcript_id="NM_001181496.1"
/db_xref="GeneID:853382"
CDS complement(315759..316475)
/gene="MRPL8"
/locus_tag="YJL063C"
/gene_synonym="bL17m; HRD238"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:12392552]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:12392552]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005762
mitochondrial large ribosomal subunit [PMID:12392552]"
/note="Mitochondrial ribosomal protein of the large
subunit"
/codon_start=1
/product="mitochondrial 54S ribosomal protein bL17m MRPL8"
/protein_id="NP_012472.1"
/db_xref="GeneID:853382"
/db_xref="SGD:S000003599"
/translation="MTVGIARKLSRDKAHRDALLKNLACQLFQHESIVSTHAKCKEAS
RVAERIITWTKRAITTSNSVAQAELKSQIQSQLFLAGDNRKLMKRLFSEIAPRYLERP
GGYTRVLRLEPRANDSAPQSVLELVDSPVMSESHTVNRGNLKMWLLVKSVINDDANQL
PHNPLTLQNLHKVAKFKAEAQLHGEIMLIKQVLLKEMSLPYDEALENERTQALLKEVY
SSSLPKKTKKPSSYVMVPRP"
gene <316723..>316980
/gene="COA3"
/locus_tag="YJL062W-A"
/gene_synonym="COX25; RRG10"
/db_xref="GeneID:853383"
mRNA <316723..>316980
/gene="COA3"
/locus_tag="YJL062W-A"
/gene_synonym="COX25; RRG10"
/product="Coa3p"
/transcript_id="NM_001184477.3"
/db_xref="GeneID:853383"
CDS 316723..316980
/gene="COA3"
/locus_tag="YJL062W-A"
/gene_synonym="COX25; RRG10"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane
[PMID:21068384|PMID:20876281]"
/experiment="EXISTENCE:mutant phenotype:GO:0033617
mitochondrial respiratory chain complex IV assembly
[PMID:21068384|PMID:20876281]"
/experiment="EXISTENCE:mutant phenotype:GO:0070130
negative regulation of mitochondrial translation
[PMID:20876281|PMID:21068384]"
/note="Mitochondrial protein required for cytochrome c
oxidase assembly; also involved in translational
regulation of Cox1p and prevention of Cox1p aggregation
before assembly; located in the mitochondrial inner
membrane"
/codon_start=1
/product="Coa3p"
/protein_id="NP_076894.3"
/db_xref="GeneID:853383"
/db_xref="SGD:S000007611"
/translation="MVLNPSKYQDTRTWKMTPAMIRARKPFFKGNMLGLTLLLGVTGS
VYYYTYHFLHKDNDFADVPIPPIDPQELEALKKEYEAKKKA"
gene <317284..>319776
/gene="LAS21"
/locus_tag="YJL062W"
/gene_synonym="GPI7"
/db_xref="GeneID:853384"
mRNA <317284..>319776
/gene="LAS21"
/locus_tag="YJL062W"
/gene_synonym="GPI7"
/product="mannose-ethanolamine phosphotransferase LAS21"
/transcript_id="NM_001181495.1"
/db_xref="GeneID:853384"
CDS 317284..319776
/gene="LAS21"
/locus_tag="YJL062W"
/gene_synonym="GPI7"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:10329735]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:10329735]"
/experiment="EXISTENCE:mutant phenotype:GO:0006506 GPI
anchor biosynthetic process [PMID:15452134|PMID:10329735]"
/experiment="EXISTENCE:mutant phenotype:GO:0051267 CP2
mannose-ethanolamine phosphotransferase activity
[PMID:15452134|PMID:10329735]"
/note="Mannose-ethanolamine phosphotransferase; involved
in biosynthesis of the glycosylphosphatidylinositol (GPI)
core structure, catalyzing the addition of a side chain
ethanolamine phosphate to the alpha1,6-linked second
mannose residue of the GPI lipid precursor; integral
component of endoplasmic reticulum membrane; mutations
affect cell wall integrity; homolog of human PIGG"
/codon_start=1
/product="mannose-ethanolamine phosphotransferase LAS21"
/protein_id="NP_012473.1"
/db_xref="GeneID:853384"
/db_xref="SGD:S000003598"
/translation="MNLKQFTCLSCAQLLAILLFIFAFFPRKIVLTGISKQDPDQDRD
LQRDRPFQKLVFVIIDALRSDFLFDSQISHFNNVHQWLNTGEAWGYTSFANPPTVTLP
RLKSITTGSTPSFIDLLLNVAQDIDSNDLSEHDSWLQQFIQHNNTIRFMGDDTWLKLF
PQQWFDFADPTHSFFVSDFTQVDNNVTRNLPGKLFQEWAQWDVAILHYLGLDHIGHKD
GPHSKFMAAKHQEMDSILKSIYDEVLEHEDDDDTLICVLGDHGMNELGNHGGSSAGET
SAGLLFLSPKLAQFARPESQVNYTLPINASPDWNFQYLETVQQIDIVPTIAALFGMPI
PMNSVGIIIPDFLQLLPNKLASMKENFMHLWKLSDHHGEVALDDFTAEDIYTKMYTIQ
ETLTKSATNYNYPLLTLAFVGFLIITIIAIYVLLRYSGPDFWQLRVSSLSVLLVSIIL
GVSTFASSFIEEEHQLWWWIVTAFSAVPLFVYRLNVLIIVRWFIMMACVRSIKFWNNS
GQKFIYSNVMSNLLNQNPSWKWCLNMLTFLVLIMASAGFQVLHFIVTTILVGLCFTYK
ISWEIVNGNQAEIPLFMHDLLAKIDFAPTESNLIVLARVFFQAWAIVVISRLVLTKLK
VLNKNYLIKDMKVYITILLMFQTSSQNIGQFLVFQILESQIFYFFQNIPTASLTSTSK
IYFSNLVSLILQNFTFFQFGGTNSISTIDLGNAYHGVSSDYNIYVVGILMSVANFAPA
IYWSMLPWSINYASIPAQVKLQTFIRSKLPAFTYHCIFGTCLMTACVVLRFHLFIWSV
FSPKLCYFLGWNFVMGLLNGWLPELALLCALD"
gene <320016..>322157
/gene="NUP82"
/locus_tag="YJL061W"
/gene_synonym="HRB187"
/db_xref="GeneID:853385"
mRNA <320016..>322157
/gene="NUP82"
/locus_tag="YJL061W"
/gene_synonym="HRB187"
/product="linker nucleoporin NUP82"
/transcript_id="NM_001181494.1"
/db_xref="GeneID:853385"
CDS 320016..322157
/gene="NUP82"
/locus_tag="YJL061W"
/gene_synonym="HRB187"
/experiment="EXISTENCE:curator inference:GO:0017056
structural constituent of nuclear pore [PMID:18046406]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005643 nuclear
pore [PMID:7559751|PMID:10684247]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0044612 nuclear
pore linkers [PMID:18046406]"
/experiment="EXISTENCE:direct assay:GO:0044614 nuclear
pore cytoplasmic filaments [PMID:10684247|PMID:9736720]"
/experiment="EXISTENCE:mutant phenotype:GO:0000055
ribosomal large subunit export from nucleus
[PMID:11739405|PMID:11071906]"
/experiment="EXISTENCE:mutant phenotype:GO:0000056
ribosomal small subunit export from nucleus
[PMID:11739405|PMID:10465789]"
/experiment="EXISTENCE:mutant phenotype:GO:0006406 mRNA
export from nucleus [PMID:7559751|PMID:7559750]"
/experiment="EXISTENCE:mutant phenotype:GO:0006606 protein
import into nucleus [PMID:15294903]"
/experiment="EXISTENCE:mutant phenotype:GO:0006611 protein
export from nucleus [PMID:15294903|PMID:17347149]"
/note="Linker nucleoporin component of the nuclear pore
complex (NPC); also part of the NPC cytoplasmic filaments;
contributes to nucleocytoplasmic transport and NPC
biogenesis; forms stable associations with three
FG-nucleoporins (Nsp1p, Nup159p, and Nup116p); relocalizes
to the cytosol in response to hypoxia"
/codon_start=1
/product="linker nucleoporin NUP82"
/protein_id="NP_012474.1"
/db_xref="GeneID:853385"
/db_xref="SGD:S000003597"
/translation="MSQSSRLSALPIFQASLSASQSPRYIFSSQNGTRIVFIQDNIIR
WYNVLTDSLYHSLNFSRHLVLDDTFHVISSTSGDLLCLFNDNEIFVMEVPWGYSNVED
VSIQDAFQIFHYSIDEEEVGPKSSIKKVLFHPKSYRDSCIVVLKEDDTITMFDILNSQ
EKPIVLNKPNNSFGLDARVNDITDLEFSKDGLTLYCLNTTEGGDIFAFYPFLPSVLLL
NEKDLNLILNKSLVMYESLDSTTDVIVKRNVIKQLQFVSKLHENWNSRFGKVDIQKEY
RLAKVQGPFTINPFPGELYDYTATNIATILIDNGQNEIVCVSFDDGSLILLFKDLEMS
MSWDVDNYVYNNSLVLIERVKLQREIKSLITLPEQLGKLYVISDNIIQQVNFMSWAST
LSKCINESDLNPLAGLKFESKLEDIATIERIPNLAYINWNDQSNLALMSNKTLTFQNI
SSDMKPQSTAAETSISTEKSDTVGDGFKMSFTQPINEILILNDNFQKACISPCERIIP
SADRQIPLKNEASENQLEIFTDISKEFLQRIVKAQTLGVSIHNRIHEQQFELTRQLQS
TCKIISKDDDLRRKFEAQNKKWDAQLSRQSELMERFSKLSKKLSQIAESNKFKEKKIS
HGEMKWFKEIRNQILQFNSFVHSQKSLQQDLSYLKSELTRIEAETIKVDKKSQNEWDE
LRKMLEIDSKIIKECNEELLQVSQEFTTKTQ"
gene <323386..>324720
/gene="BNA3"
/locus_tag="YJL060W"
/db_xref="GeneID:853386"
mRNA <323386..>324720
/gene="BNA3"
/locus_tag="YJL060W"
/product="kynurenine--oxoglutarate transaminase"
/transcript_id="NM_001181493.3"
/db_xref="GeneID:853386"
CDS 323386..324720
/gene="BNA3"
/locus_tag="YJL060W"
/EC_number="2.6.1.7"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:17034789]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:24769239|PMID:14562095|PMID:16823961|PMID:17034789]"
/experiment="EXISTENCE:direct assay:GO:0016212
kynurenine-oxoglutarate transaminase activity
[PMID:18205391]"
/experiment="EXISTENCE:direct assay:GO:0034276 kynurenic
acid biosynthetic process [PMID:18205391]"
/experiment="EXISTENCE:direct assay:GO:0047536
2-aminoadipate transaminase activity [PMID:19342587]"
/note="Kynurenine aminotransferase; catalyzes formation of
kynurenic acid from kynurenine; potential Cdc28p
substrate"
/codon_start=1
/product="kynurenine--oxoglutarate transaminase"
/protein_id="NP_012475.3"
/db_xref="GeneID:853386"
/db_xref="SGD:S000003596"
/translation="MKQRFIRQFTNLMSTSRPKVVANKYFTSNTAKDVWSLTNEAAAK
AANNSKNQGRELINLGQGFFSYSPPQFAIKEAQKALDIPMVNQYSPTRGRPSLINSLI
KLYSPIYNTELKAENVTVTTGANEGILSCLMGLLNAGDEVIVFEPFFDQYIPNIELCG
GKVVYVPINPPKELDQRNTRGEEWTIDFEQFEKAITSKTKAVIINTPHNPIGKVFTRE
ELTTLGNICVKHNVVIISDEVYEHLYFTDSFTRIATLSPEIGQLTLTVGSAGKSFAAT
GWRIGWVLSLNAELLSYAAKAHTRICFASPSPLQEACANSINDALKIGYFEKMRQEYI
NKFKIFTSIFDELGLPYTAPEGTYFVLVDFSKVKIPEDYPYPEEILNKGKDFRISHWL
INELGVVAIPPTEFYIKEHEKAAENLLRFAVCKDDAYLENAVERLKLLKDYL"
gene <324964..>326190
/gene="YHC3"
/locus_tag="YJL059W"
/gene_synonym="BTN1"
/db_xref="GeneID:853387"
mRNA <324964..>326190
/gene="YHC3"
/locus_tag="YJL059W"
/gene_synonym="BTN1"
/product="amino acid transporter YHC3"
/transcript_id="NM_001181492.1"
/db_xref="GeneID:853387"
CDS 324964..326190
/gene="YHC3"
/locus_tag="YJL059W"
/gene_synonym="BTN1"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:10319861|PMID:9753630]"
/experiment="EXISTENCE:mutant phenotype:GO:0015819 lysine
transport [PMID:14660799]"
/experiment="EXISTENCE:mutant phenotype:GO:0051453
regulation of intracellular pH
[PMID:10319861|PMID:16423829|PMID:10191121]"
/experiment="EXISTENCE:mutant phenotype:GO:1903826
L-arginine transmembrane transport [PMID:14660799]"
/note="Protein required for the ATP-dependent transport of
arginine; vacuolar membrane protein; involved in the
ATP-dependent transport of arginine into the vacuole and
possibly in balancing ion homeostasis; human homolog CLN3
involved in Batten disease (juvenile onset neuronal ceroid
lipofuscinosis) can complement yeast null mutant"
/codon_start=1
/product="amino acid transporter YHC3"
/protein_id="NP_012476.1"
/db_xref="GeneID:853387"
/db_xref="SGD:S000003595"
/translation="MSDKSHQIYCYFWLFGLINNVLYVVILSAAVDIVGPTLPKSLVL
LADIFPSLAIKLCSPFFIDRIKYSYRIWSLITMSCLGMFLVSFKNLFVCLLGISFASI
SSGFGEVTFLQLTHYYKQISLNGWSSGTGGAGIIGGASYMFLTSIFKVPVKLTLLVFS
LLPFAFLFYFKLESNDTNLTYQSLQQIDEAEDDQLVPFPVAFTHTNASQSLYSTRQHI
LQTVKRLRRLVFPYMVPLTTVYLFEYLINQAVAPTLLFPINGDERSKSMPFFFHKYRD
IYVTYGTLYQLGVFISRSFGHLMRMRSLYILAFLQGVNLCITVLQSWFYVTHSPWAVM
ILIFYEGFLGGASYVNTFLNILEQEDPDETEFAMGAVSIADSFGVFLAALLGLGLEPK
LCRHQIADDRPWCRME"
gene complement(<326242..>327873)
/gene="BIT61"
/locus_tag="YJL058C"
/db_xref="GeneID:853388"
mRNA complement(<326242..>327873)
/gene="BIT61"
/locus_tag="YJL058C"
/product="Bit61p"
/transcript_id="NM_001181491.1"
/db_xref="GeneID:853388"
CDS complement(326242..327873)
/gene="BIT61"
/locus_tag="YJL058C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:19144819]"
/experiment="EXISTENCE:direct assay:GO:0031932 TORC2
complex [PMID:14736892]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:physical interaction:GO:0001558
regulation of cell growth [PMID:14736892]"
/experiment="EXISTENCE:physical interaction:GO:0030950
establishment or maintenance of actin cytoskeleton
polarity [PMID:14736892]"
/experiment="EXISTENCE:physical interaction:GO:0031505
fungal-type cell wall organization [PMID:14736892]"
/note="Subunit of TORC2, a regulator of plasma membrane
(PM) homeostasis; TORC2 also regulates actin cytoskeletal
dynamics during polarized growth and cell wall integrity;
interacts with Slm1p and Slm2p, homologous PH
domain-containing TORC2 substrates; PM retention is
dependent on Avo3p; BIT61 has a paralog, BIT2, that arose
from the whole genome duplication"
/codon_start=1
/product="Bit61p"
/protein_id="NP_012477.1"
/db_xref="GeneID:853388"
/db_xref="SGD:S000003594"
/translation="MTAEDILLRERTSTTTQRPVNSEQYLNVQLATAPVKNFQTTSEI
SRQTLVDTSNDDVYSIKNLKGSRNPISPSVSNVGFQSIFHTVDHPRSKVSVASNHSLR
SNDNASAATSKSGSSQIGESHSVDTVECSNNLSKKLSSDAISITQKSLHSTPSGRYMK
GKASGFFNRRNRAHTTISSDPASFLTDSSTLHNSSHSFRNVIKNFFQNKSHRHIGQDA
IEPAIPNSLSKFLHSSYGRHKSPSQFIHTNAGQLVDSGTSVYSLNVNPSGVNPNTIVE
DPLSGTDPASPNPVSMLHDLLRNLPSLEANYKHFNSQELTTLTNNIWNIFCSNVAELF
RTQRIWKLRAKIENFNEVLEFYCILKTDPRVTHSGMNRIISDLKEFLVSSLYNLENQI
VFNYSNEDTINNALKRLGVIWRIFYQEVYYDLAAVLLPLDQSIREDGNSTVLKSGNES
RTHINGNYSIGFLLLMCFRDSIVLPCYENFVNSNDGISKSFQLYIFNQEEESNVTETD
KLTLLQCFGILSTIQSNDRNQRIIEELLAGIRMSI"
gene complement(<328118..>330121)
/gene="IKS1"
/locus_tag="YJL057C"
/db_xref="GeneID:853389"
mRNA complement(<328118..>330121)
/gene="IKS1"
/locus_tag="YJL057C"
/product="protein kinase IKS1"
/transcript_id="NM_001181490.3"
/db_xref="GeneID:853389"
CDS complement(328118..330121)
/gene="IKS1"
/locus_tag="YJL057C"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0006468 protein
phosphorylation [PMID:16319894]"
/note="Protein kinase of unknown cellular role; putative
serine/threonine kinase; expression is induced during mild
heat stress; deletion mutants are hypersensitive to copper
sulphate and resistant to sorbate; interacts with an
N-terminal fragment of Sst2p"
/codon_start=1
/product="protein kinase IKS1"
/protein_id="NP_012478.3"
/db_xref="GeneID:853389"
/db_xref="SGD:S000003593"
/translation="MSLVPYEEGSLILDDPNSKSVVVVNPTSGTLSFFQQDNSNNDSE
LNEDQTASLSALDFPSGIHQYKSPIASYVCPQCGTEINPDIINRRQLHRRASAGVESE
SSRLSIPENTVPLGFEFANSSFSRRYFQSLERNHRHYALQNDSNNKQGQFSKNKYFIP
DDLFIPGYFRKFFKILSLLGNGARGSVYKVVHTIGNTELGVFALKKIPIGNDMEWFNK
CIREVKALSSLTHKSANLITYNHVWLEMDSSVGFVRSIDGSQSDSQEEVPCIFILQQY
CSGGNLEDCILRKVFNRFSDTESPEERKKKFRTRKKNHGKSGEVGLSTEQLVSIIRDI
ARGLHELHSIGLIHRDLKPSNCLLLTPFKSDNENDNVYDREHNSDEFFPSIVIGDLGE
SQLEGESRLGTGCTGTLEFTAPDLIIQGRPVSSSTLPSRSSHTYNEYTFASDMYSLGM
ICYFIVFGELPFEPQLDIVDLKVRIKNFRFDTEGMIEKHQAMKLKPIDRRIFHLMDAL
LQPNNDARPTAKTVEETLDEMLINSKPGKKFWKENVDSTLNFSTISEVNENTNSFTDD
YIDGDNVTLSLPAPEENLSTVSTQNLQKYSALNRTIQVCYKLVSMILTIIIFKCTKTG
SWLSYMSLILLGMVFKSPADERGKHARALVLLAFIAACKKYIY"
gene complement(<330431..>333073)
/gene="ZAP1"
/locus_tag="YJL056C"
/gene_synonym="ZRG10"
/db_xref="GeneID:853390"
mRNA complement(<330431..>333073)
/gene="ZAP1"
/locus_tag="YJL056C"
/gene_synonym="ZRG10"
/product="Zap1p"
/transcript_id="NM_001181489.1"
/db_xref="GeneID:853390"
CDS complement(330431..333073)
/gene="ZAP1"
/locus_tag="YJL056C"
/gene_synonym="ZRG10"
/experiment="EXISTENCE:direct assay:GO:0000977 RNA
polymerase II transcription regulatory region
sequence-specific DNA binding [PMID:9786867]"
/experiment="EXISTENCE:direct assay:GO:0000981 DNA-binding
transcription factor activity, RNA polymerase II-specific
[PMID:22128164]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10899124]"
/experiment="EXISTENCE:direct assay:GO:0008270 zinc ion
binding [PMID:16720702|PMID:14517251]"
/experiment="EXISTENCE:direct assay:GO:0034224 cellular
response to zinc ion starvation [PMID:22128164]"
/experiment="EXISTENCE:direct assay:GO:0045944 positive
regulation of transcription by RNA polymerase II
[PMID:22128164]"
/experiment="EXISTENCE:mutant phenotype:GO:0000977 RNA
polymerase II transcription regulatory region
sequence-specific DNA binding
[PMID:12549926|PMID:9786867]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:9271382|PMID:9786867]"
/experiment="EXISTENCE:mutant phenotype:GO:0008270 zinc
ion binding [PMID:16720702]"
/experiment="EXISTENCE:mutant phenotype:GO:0034224
cellular response to zinc ion starvation
[PMID:9786867|PMID:12095998|PMID:9271382]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:12095998]"
/note="Zinc-regulated transcription factor; binds to
zinc-responsive promoters to induce transcription of
certain genes in presence of zinc, represses other genes
in low zinc; regulates its own transcription; contains
seven zinc-finger domains"
/codon_start=1
/product="Zap1p"
/protein_id="NP_012479.1"
/db_xref="GeneID:853390"
/db_xref="SGD:S000003592"
/translation="MDALTPRDSPKRDDSMATSAATAASAKPDALTIGKEGIVHGHIH
NYNNLTYIHGHLHHSAPVNDSSASATPAAAAVADAATSAFASGASHDMGGDCHVNEKC
KEYTDCQHFEFLNYHNNPSLTKYNDTATYNSNNHSFANNFHSVASDPTSPQQNSKSDL
PRRKDSWFNDDLILLPSSKKNKPNPPPGSDDCYCTPKILEICCDETHPKSEANIKQGE
SDQPTKKDISENGNDVAIFTDVKNDHLMPNFNLHDQYCNSTNHDSHNHNNTVPDSFSQ
LMSHLSEIDCDLTCDTPCTASTSATSGHKFVQDHQSSNNDDVFHKYCKFCEESTDNQP
CSKHMHLESKPPQLPPKCSSLRKPTNTLQGTNHAYHEHILNTDMDLKILEDLCNISSL
YEVPFGKHINHHDHNNAGNGCDGSSTGNNENGNQTMNLLLSSINRCNPKNNLNGSNNN
TAGATSTDHQHHHHRIQFHSHKPNRNNIVNNSGISAANTTADLTNNDLNDLISREYSY
ERFRNQSEPPSLPKVTHQNQKNRRSWPTKDLESTDFSSLEDSLPSSISPPIQTTSTIN
FNWCFKEEKNNDLKCKWKECPESCSSLFDLQRHLLKDHVSQDFKHPMEPLACNWEDCD
FLGDDTCSIVNHINCQHGINFDIQFANPDSFLPGSISKEKHHLLHCPNPQTHEVSKAD
GAPDMTSANDVSNIPPIKQPEQVICQWDGCNKSFSSAQELNDHLEAVHLTRGKSEYQC
LWHDCHRTFPQRQKLIRHLKVHSKYKPYKCKTCKRCFSSEETLVQHTRTHSGEKPYKC
HICNKKFAISSSLKIHIRTHTGEKPLQCKICGKRFNESSNLSKHIKTHQKKYKCSDCS
KSFDDLGKLNSQKVKCALERKPYL"
gene <333357..>334094
/gene="LOG1"
/locus_tag="YJL055W"
/db_xref="GeneID:853391"
mRNA <333357..>334094
/gene="LOG1"
/locus_tag="YJL055W"
/product="Log1p"
/transcript_id="NM_001181488.1"
/db_xref="GeneID:853391"
CDS 333357..334094
/gene="LOG1"
/locus_tag="YJL055W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0014074
response to purine-containing compound [PMID:15932646]"
/note="hypothetical protein; functions together with HAM1
to mediate resistance to 5-FU; specifically reduces the
incorporation of 5-FU into RNA, without affecting uptake
or incorporation of uracil into RNA; proposed to be
involved in the metabolism of purine and pyrimidine base
analogues; deletion mutants are sensitive to HAP and AHA"
/codon_start=1
/product="Log1p"
/protein_id="NP_012480.1"
/db_xref="GeneID:853391"
/db_xref="SGD:S000003591"
/translation="MTMEKNGGNSSRGGQVGGKSVCVYCGSSFGAKALYSESAEELGA
LFHKLGWKLVYGGGTTGLMGKIARSTMGPDLSGQVHGIIPNALVSKERTDEDKEDVNK
ALLESVENHKGATPISEEYGETTIVPDMHTRKRMMANLSDAFVAMPGGYGTFEEIMEC
ITWSQLGIHNKPIILFNIDGFYDKLLEFLKHSIQERFISVKNGEIIQVASTPQEVVDK
IEKYVVPEGRFNLNWSDEGHAHEDCAK"
gene <334265..>335701
/gene="TIM54"
/locus_tag="YJL054W"
/db_xref="GeneID:853392"
mRNA <334265..>335701
/gene="TIM54"
/locus_tag="YJL054W"
/product="Tim22-complex subunit TIM54"
/transcript_id="NM_001181487.3"
/db_xref="GeneID:853392"
CDS 334265..335701
/gene="TIM54"
/locus_tag="YJL054W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0008320 protein
transmembrane transporter activity [PMID:12637749]"
/experiment="EXISTENCE:direct assay:GO:0042721 TIM22
mitochondrial import inner membrane insertion complex
[PMID:10648604|PMID:10637294]"
/experiment="EXISTENCE:genetic interaction:GO:0045039
protein insertion into mitochondrial inner membrane
[PMID:9412462|PMID:10637294]"
/experiment="EXISTENCE:mutant phenotype:GO:0045039 protein
insertion into mitochondrial inner membrane
[PMID:17893242|PMID:9412462]"
/note="Component of the mitochondrial TIM22 complex;
involved in insertion of polytopic proteins into the inner
membrane"
/codon_start=1
/product="Tim22-complex subunit TIM54"
/protein_id="NP_012481.3"
/db_xref="GeneID:853392"
/db_xref="SGD:S000003590"
/translation="MSSESGKPIAKPIRKPGYTNPALKALGIPALRLPSRNWMIFWSV
LTVSIGGIAYDKYKQRQILSHATDLVKPLAEESMEVDKVPRKITVFIAPPPNDYLESS
LKVWRRYVKPVLYYAGLDYELVQEDRQGIIRTNVANRIRELRKEILASTDGQPVKEPN
QTVAKPSGSSTSKISSLLPFNKIIQDPAEEDDSFDPEIGKKFKENFDWRNVIGIFYTM
PKPKHIISEDALTKDPILSGGVICLGRGAYKEYIAGIHEGLLGPIEKTEKTGSTEPKM
TGVVEANQIESKVSESGATELVDAEKETALEEAKVQDDLKVDEENSSEDSQKFLKPFI
SSDQYPDLQIASELQTPNGEFIRNPNTNIPLLINQPLLVIPIPNLIGFTTIPRRIHRF
YQKRFYVEDVCSSVVNCVRQTRIRPFDIAKDIDLAKDEEKDWPQNWVKQGKEKNSEWT
QELVCDPRITKHMFVYEKPPKEEPESDI"
gene <335898..>337037
/gene="PEP8"
/locus_tag="YJL053W"
/gene_synonym="GRD6; VPS26; VPT4"
/db_xref="GeneID:853393"
mRNA <335898..>337037
/gene="PEP8"
/locus_tag="YJL053W"
/gene_synonym="GRD6; VPS26; VPT4"
/product="retromer subunit PEP8"
/transcript_id="NM_001181486.1"
/db_xref="GeneID:853393"
CDS 335898..337037
/gene="PEP8"
/locus_tag="YJL053W"
/gene_synonym="GRD6; VPS26; VPT4"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:9700157]"
/experiment="EXISTENCE:direct assay:GO:0170071 CROP
complex [PMID:35466426]"
/experiment="EXISTENCE:mutant phenotype:GO:0030904
retromer complex [PMID:9700157]"
/experiment="EXISTENCE:mutant phenotype:GO:0030906
retromer, cargo-selective complex [PMID:9700157]"
/experiment="EXISTENCE:mutant phenotype:GO:0045053 protein
retention in Golgi apparatus [PMID:8649377]"
/experiment="EXISTENCE:mutant phenotype:GO:0140318 protein
transporter activity [PMID:9700157]"
/experiment="EXISTENCE:physical interaction:GO:0030904
retromer complex [PMID:9700157]"
/experiment="EXISTENCE:physical interaction:GO:0042147
retrograde transport, endosome to Golgi [PMID:9700157]"
/experiment="EXISTENCE:physical interaction:GO:0140318
protein transporter activity [PMID:9700157]"
/note="Vacuolar protein component of the retromer; forms
part of the multimeric membrane-associated retromer
complex involved in vacuolar protein sorting along with
Vps35p, Vps29p, Vps17p, and Vps5p; essential for
endosome-to-Golgi retrograde protein transport; interacts
with Ypt7p; protein abundance increases in response to DNA
replication stress"
/codon_start=1
/product="retromer subunit PEP8"
/protein_id="NP_012482.1"
/db_xref="GeneID:853393"
/db_xref="SGD:S000003589"
/translation="MSIFFKPPIDIEILFDNEESRKHVDIATRSSNSSYKSMKESLPV
YEDGESLGGIVTLRVRDSKKVDHLGIKVSVIGSIDMLKSHGSGNSSSKKVTSSTSSSS
SNGSVDVRKNSVDQFLCQSYDLCPAGELQHSQSFPFLFRDLSKRYESYKGKNVDVAYY
VKVTVMRKSTDISKIKRFWVYLYNSVTTAPNTLSANETKATTNDIAGGNYAADNASDN
TQTKSTQGEAADVNQVLPISHSNNEPKPVRLDIGIENCLHIEFEYAKSQYSLKEVIVG
RIYFLLTRLRIKHMELSLITRESSGLQTSNVMTDSTAIRYEIMDGSSVKGETIPIRLF
LSGYDLTPNMSCNYFNVKNYLSLVIIDEDGRRYFKQSEITLYRTR"
rep_origin 337281..337529
/note="ARS1011; Autonomously Replicating Sequence"
/db_xref="SGD:S000028448"
gene complement(<337884..>338003)
/locus_tag="YJL052C-A"
/db_xref="GeneID:853394"
mRNA complement(<337884..>338003)
/locus_tag="YJL052C-A"
/product="uncharacterized protein"
/transcript_id="NM_001184476.1"
/db_xref="GeneID:853394"
CDS complement(337884..338003)
/locus_tag="YJL052C-A"
/note="hypothetical protein; identified based on
comparison to related yeast species; mCherry fusion
protein localizes to the vacuole"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_076895.1"
/db_xref="GeneID:853394"
/db_xref="SGD:S000007610"
/translation="MHLRSRWWLALLYCKDPVSRSATTPKVETRASCLLSRAF"
gene <338271..>339269
/gene="TDH1"
/locus_tag="YJL052W"
/gene_synonym="GLD3"
/db_xref="GeneID:853395"
mRNA <338271..>339269
/gene="TDH1"
/locus_tag="YJL052W"
/gene_synonym="GLD3"
/product="glyceraldehyde-3-phosphate dehydrogenase
(phosphorylating) TDH1"
/transcript_id="NM_001181485.3"
/db_xref="GeneID:853395"
CDS 338271..339269
/gene="TDH1"
/locus_tag="YJL052W"
/gene_synonym="GLD3"
/EC_number="1.2.1.12"
/experiment="EXISTENCE:direct assay:GO:0004365
glyceraldehyde-3-phosphate dehydrogenase (NAD+)
(phosphorylating) activity [PMID:3905788]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16962558]"
/experiment="EXISTENCE:direct assay:GO:0005811 lipid
droplet [PMID:10515935]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762|PMID:11158358]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:11158358]"
/experiment="EXISTENCE:direct assay:GO:1904408 melatonin
binding [PMID:31708896]"
/experiment="EXISTENCE:expression pattern:GO:0006094
gluconeogenesis [PMID:3905788]"
/experiment="EXISTENCE:expression pattern:GO:0006096
glycolytic process [PMID:3905788]"
/experiment="EXISTENCE:physical interaction:GO:0005739
mitochondrion [PMID:16962558]"
/note="Glyceraldehyde-3-phosphate dehydrogenase (GAPDH),
isozyme 1; involved in glycolysis and gluconeogenesis;
tetramer that catalyzes the reaction of
glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate;
detected in the cytoplasm and cell wall; protein abundance
increases in response to DNA replication stress;
GAPDH-derived antimicrobial peptides secreted by S.
cerevisiae are active against a wide variety of
wine-related yeasts and bacteria"
/codon_start=1
/product="glyceraldehyde-3-phosphate dehydrogenase
(phosphorylating) TDH1"
/protein_id="NP_012483.3"
/db_xref="GeneID:853395"
/db_xref="SGD:S000003588"
/translation="MIRIAINGFGRIGRLVLRLALQRKDIEVVAVNDPFISNDYAAYM
VKYDSTHGRYKGTVSHDDKHIIIDGVKIATYQERDPANLPWGSLKIDVAVDSTGVFKE
LDTAQKHIDAGAKKVVITAPSSSAPMFVVGVNHTKYTPDKKIVSNASCTTNCLAPLAK
VINDAFGIEEGLMTTVHSMTATQKTVDGPSHKDWRGGRTASGNIIPSSTGAAKAVGKV
LPELQGKLTGMAFRVPTVDVSVVDLTVKLEKEATYDQIKKAVKAAAEGPMKGVLGYTE
DAVVSSDFLGDTHASIFDASAGIQLSPKFVKLISWYDNEYGYSARVVDLIEYVAKA"
gene <339787..>342255
/gene="IRC8"
/locus_tag="YJL051W"
/db_xref="GeneID:853396"
mRNA <339787..>342255
/gene="IRC8"
/locus_tag="YJL051W"
/product="Irc8p"
/transcript_id="NM_001181484.1"
/db_xref="GeneID:853396"
CDS 339787..342255
/gene="IRC8"
/locus_tag="YJL051W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005933 cellular
bud [PMID:13679573]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0006312 mitotic
recombination [PMID:18085829]"
/note="Bud tip localized hypothetical protein; mRNA is
targeted to the bud by a She2p dependent transport system;
mRNA is cell cycle regulated via Fkh2p, peaking in G2/M
phase; null mutant displays increased levels of
spontaneous Rad52p foc"
/codon_start=1
/product="Irc8p"
/protein_id="NP_012484.1"
/db_xref="GeneID:853396"
/db_xref="SGD:S000003587"
/translation="MCHNSVRSGNKAGFLGIKFGSALLSIATGAIAIALLCKFHDHEA
VLIVIVCSTLLYGIPSLISFITETVFAPSKFHIGYFYNVLNFALPLITMGCTVDYFHN
TLRSPISVQSESHRVYITTLDSLLIFTLFINGIQLGFFLKDGNANNFGSSSNNISTDQ
YDKEANAVENGRFVPLKNSSQTLTPDLELLHGSPKSMNGVAWLINELSTNSNTNANKT
ISSDENSNSSVIRHKLGPISTSKCPKKPSHSHFSKLKKYNSFFLGPKENRYKRNTQQA
TKVPTEKKSNHRSSQYVSRLSTISDISKSFLNFLALNEKNGNSTSTARTPSEGRVSII
INEGNNTLKYKTPHDSHTIDSPNLELEREAIGRINSALLPACLRVTDKMISPQQSTQN
EDSYQATPLIPQVEVDDDFYVGDILMTNELQDIPQVPRISSDIEDDFEQQYTKHVDLP
ARVTLEMWEKDQEKILQKVTTNRDKSKLLPPFRFTSESDMDPSTSTELEVELHAQNNF
SFPFKSAGLQIATSDQFNQQEFKTSDTISELDEYLHDPSIQEEDASQLIESSLNQNNL
SSTTIDNGPKDMSRFSTRHSPTKSIISMISGSGSVKHQHSHSTLSNFFTGHSRNNSQI
NQLLQGSSSNMMSNTSPHSSPTKSLRMRFGKKLSLSNISDTMSPYEGSTTDPINYSFG
HGHNKNQSIDFSYVRTLQSSHSPTKSTSGNSRRDSLNNDRTQSTVNERALRTASTLFY
LQHNNATCTLNGEEPVLDTPQSIQSSSSGSEQESAGSGSGYPEVVFSEYDREKWNVLR
NLKEIAPEKTIESGPVEELVSPSK"
gene <342522..>345743
/gene="MTR4"
/locus_tag="YJL050W"
/gene_synonym="DOB1"
/db_xref="GeneID:853397"
mRNA <342522..>345743
/gene="MTR4"
/locus_tag="YJL050W"
/gene_synonym="DOB1"
/product="ATP-dependent RNA helicase MTR4"
/transcript_id="NM_001181483.1"
/db_xref="GeneID:853397"
CDS 342522..345743
/gene="MTR4"
/locus_tag="YJL050W"
/gene_synonym="DOB1"
/EC_number="3.6.4.13"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:8756671|PMID:15226447]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:16541108]"
/experiment="EXISTENCE:direct assay:GO:0008143 poly(A)
binding [PMID:18096702]"
/experiment="EXISTENCE:direct assay:GO:0031499 TRAMP
complex [PMID:15935759|PMID:15828860|PMID:15935758]"
/experiment="EXISTENCE:direct assay:GO:0034458 3'-5' RNA
helicase activity [PMID:18000032|PMID:18096702]"
/experiment="EXISTENCE:direct assay:GO:0071038
TRAMP-dependent tRNA surveillance pathway
[PMID:17643380|PMID:15828860|PMID:15935758]"
/experiment="EXISTENCE:genetic interaction:GO:0071028
nuclear mRNA surveillance [PMID:11586364]"
/experiment="EXISTENCE:genetic interaction:GO:0071038
TRAMP-dependent tRNA surveillance pathway [PMID:18000032]"
/experiment="EXISTENCE:mutant phenotype:GO:0000467
exonucleolytic trimming to generate mature 3'-end of 5.8S
rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S
rRNA, LSU-rRNA) [PMID:10508172|PMID:9463390]"
/experiment="EXISTENCE:mutant phenotype:GO:0006397 mRNA
processing [PMID:27385342]"
/experiment="EXISTENCE:mutant phenotype:GO:0016075 rRNA
catabolic process [PMID:10508172|PMID:9463390]"
/experiment="EXISTENCE:mutant phenotype:GO:0034458 3'-5'
RNA helicase activity [PMID:18000032]"
/experiment="EXISTENCE:mutant phenotype:GO:0034475 U4
snRNA 3'-end processing [PMID:10508172|PMID:10611222]"
/experiment="EXISTENCE:mutant phenotype:GO:0034476 U5
snRNA 3'-end processing [PMID:10508172]"
/experiment="EXISTENCE:mutant phenotype:GO:0071031 nuclear
mRNA surveillance of mRNA 3'-end processing
[PMID:17410208]"
/experiment="EXISTENCE:mutant phenotype:GO:0071035 nuclear
polyadenylation-dependent rRNA catabolic process
[PMID:16374505|PMID:18940861]"
/experiment="EXISTENCE:mutant phenotype:GO:0071042 nuclear
polyadenylation-dependent mRNA catabolic process
[PMID:19369424]"
/experiment="EXISTENCE:mutant phenotype:GO:0071051
poly(A)-dependent snoRNA 3'-end processing
[PMID:10611222]"
/experiment="EXISTENCE:physical interaction:GO:0031499
TRAMP complex [PMID:15935759]"
/note="RNA duplex-sensing translocase; ATP-dependent 3'-5'
RNA helicase of the DExD/H family; involved in nuclear RNA
processing and degradation as a component of TRAMP complex
and in TRAMP-independent processes; TRAMP unwinds RNA
duplexes, with Mtr4p unwinding activity stimulated by
Pap2p/Air2p but not dependent on ongoing polyadenylation;
contains an arch domain, with two coiled-coil arms/stalks
and a globular fist/KOW domain, which has RNA binding
activity and is required for 5.8S rRNA processing"
/codon_start=1
/product="ATP-dependent RNA helicase MTR4"
/protein_id="NP_012485.1"
/db_xref="GeneID:853397"
/db_xref="SGD:S000003586"
/translation="MDSTDLFDVFEETPVELPTDSNGEKNADTNVGDTPDHTQDKKHG
LEEEKEEHEENNSENKKIKSNKSKTEDKNKKVVVPVLADSFEQEASREVDASKGLTNS
ETLQVEQDGKVRLSHQVRHQVALPPNYDYTPIAEHKRVNEARTYPFTLDPFQDTAISC
IDRGESVLVSAHTSAGKTVVAEYAIAQSLKNKQRVIYTSPIKALSNQKYRELLAEFGD
VGLMTGDITINPDAGCLVMTTEILRSMLYRGSEVMREVAWVIFDEVHYMRDKERGVVW
EETIILLPDKVRYVFLSATIPNAMEFAEWICKIHSQPCHIVYTNFRPTPLQHYLFPAH
GDGIYLVVDEKSTFREENFQKAMASISNQIGDDPNSTDSRGKKGQTYKGGSAKGDAKG
DIYKIVKMIWKKKYNPVIVFSFSKRDCEELALKMSKLDFNSDDEKEALTKIFNNAIAL
LPETDRELPQIKHILPLLRRGIGIHHSGLLPILKEVIEILFQEGFLKVLFATETFSIG
LNMPAKTVVFTSVRKWDGQQFRWVSGGEYIQMSGRAGRRGLDDRGIVIMMIDEKMEPQ
VAKGMVKGQADRLDSAFHLGYNMILNLMRVEGISPEFMLEHSFFQFQNVISVPVMEKK
LAELKKDFDGIEVEDEENVKEYHEIEQAIKGYREDVRQVVTHPANALSFLQPGRLVEI
SVNGKDNYGWGAVVDFAKRINKRNPSAVYTDHESYIVNVVVNTMYIDSPVNLLKPFNP
TLPEGIRPAEEGEKSICAVIPITLDSIKSIGNLRLYMPKDIRASGQKETVGKSLREVN
RRFPDGIPVLDPVKNMKIEDEDFLKLMKKIDVLNTKLSSNPLTNSMRLEELYGKYSRK
HDLHEDMKQLKRKISESQAVIQLDDLRRRKRVLRRLGFCTPNDIIELKGRVACEISSG
DELLLTELIFNGNFNELKPEQAAALLSCFAFQERCKEAPRLKPELAEPLKAMREIAAK
IAKIMKDSKIEVVEKDYVESFRHELMEVVYEWCRGATFTQICKMTDVYEGSLIRMFKR
LEELVKELVDVANTIGNSSLKEKMEAVLKLIHRDIVSAGSLYL"
gene <345973..>347325
/gene="CHM7"
/locus_tag="YJL049W"
/db_xref="GeneID:853398"
mRNA <345973..>347325
/gene="CHM7"
/locus_tag="YJL049W"
/product="phosphatidic acid-binding protein CHM7"
/transcript_id="NM_001181482.3"
/db_xref="GeneID:853398"
CDS 345973..347325
/gene="CHM7"
/locus_tag="YJL049W"
/experiment="EXISTENCE:direct assay:GO:0005637 nuclear
inner membrane [PMID:33464310]"
/experiment="EXISTENCE:direct assay:GO:0070300
phosphatidic acid binding [PMID:33464310]"
/experiment="EXISTENCE:mutant phenotype:GO:0034727
piecemeal microautophagy of the nucleus [PMID:39721364]"
/experiment="EXISTENCE:mutant phenotype:GO:0051292 nuclear
pore complex assembly [PMID:33464310]"
/experiment="EXISTENCE:mutant phenotype:GO:0070550 rDNA
chromatin condensation [PMID:39721364]"
/note="Phosphatidic acid (PA)-binding nuclear envelope
(NE)-specific ESCRT; crucial for survival of quiescent
cells in prolonged nutrient-starved conditions; homolog of
human CHMP7, localizes to the endoplasmic reticulum
presumably as part of ESCRT-III like complex"
/codon_start=1
/product="phosphatidic acid-binding protein CHM7"
/protein_id="NP_012486.3"
/db_xref="GeneID:853398"
/db_xref="SGD:S000003585"
/translation="MKVDLPESRLPSLYRDFRPLKDLNPDGYEANISTWRDFFLQQYI
SNSNRIIFTIGTRTLQELTHEVYGVPKSIDIAIDVLVNEGNLIPMELFNLGGMYTDNS
RKGFWKWVRSWKESTNMYKSRKDETNFYLREDKFIIKANLEKEYQRFHELLKRSVFME
ASSITDLVFTRNEFVTTESLQSFFSTYDEETKNVFLHFIENYKHIIVSKDNVIKVIAA
EVEDITSRFSKGITENDLRIASVKVGIININKQITRLRKEINESNIKLRGPEFNELPK
RIRIEYKQARLLSEKHLSRLLKFQNNLAQVRTQIDTSATNAVLIQTLSESNEVIKSIN
GYIGSTEKVEDLLDEIKEGHDRTEEVNDLLAHYNKGQDEEAEEEIERELEQLELDEKN
NNKEENKNQDLHEPKESSSEDLLKRLNNLKINTNEGPVQDNENHDNEIRKIMMEEQPR
"
gene complement(<347447..>348637)
/gene="UBX6"
/locus_tag="YJL048C"
/gene_synonym="CUI2"
/db_xref="GeneID:853399"
mRNA complement(<347447..>348637)
/gene="UBX6"
/locus_tag="YJL048C"
/gene_synonym="CUI2"
/product="Ubx6p"
/transcript_id="NM_001181481.1"
/db_xref="GeneID:853399"
CDS complement(347447..348637)
/gene="UBX6"
/locus_tag="YJL048C"
/gene_synonym="CUI2"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14755638]"
/experiment="EXISTENCE:mutant phenotype:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:14755638]"
/experiment="EXISTENCE:mutant phenotype:GO:0030435
sporulation resulting in formation of a cellular spore
[PMID:14755638]"
/note="UBX (ubiquitin regulatory X) domain-containing
protein; interacts with Cdc48p, transcription is repressed
when cells are grown in media containing inositol and
choline; UBX6 has a paralog, UBX7, that arose from the
whole genome duplication"
/codon_start=1
/product="Ubx6p"
/protein_id="NP_012487.1"
/db_xref="GeneID:853399"
/db_xref="SGD:S000003584"
/translation="MYEMSGIDSLFHDRVVHDYSHTSEQVIVVYISSAAGDNSWLHQW
FKPGNLSDEERENILWVRLVNGTKECLLFKSIFPSSSAPSINILQNGLLECSIQGNSL
SREQDPWETFINGLQSVFKGQVTKRKLFSKSNEEYQRVKRMIQNDKLERKYVFQNTND
PQRKPQKWKQLTVTDNVSYKSQKGFLAQNYCTLQLKLPNGYTISNTFPPQTKLHKVRM
WLDYNCYDDGTPYLFHRNIPRVTLTRNDELKSLQELDLLPRSTLILEPLEANNKTFDY
MEQSSLLHKVYSGLTSFWAKEPEVDASSSRLGYQRLGTNVSNSANYSLQKLSSLDMVS
DGGGGGGGDSMTPSAYTTPRMYPSNGTSQLRQNVSELNLSSNNSASNTKVRTLGYSNN
NGNN"
gene complement(<349044..>349178)
/locus_tag="YJL047C-A"
/db_xref="GeneID:1466468"
mRNA complement(<349044..>349178)
/locus_tag="YJL047C-A"
/product="uncharacterized protein"
/transcript_id="NM_001184635.1"
/db_xref="GeneID:1466468"
CDS complement(349044..349178)
/locus_tag="YJL047C-A"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878105.1"
/db_xref="GeneID:1466468"
/db_xref="SGD:S000028804"
/translation="MKIKISIEISLSLLSEHYKRNENCISNMLVIGEGPRGETIVERF
"
gene complement(349130..349233)
/gene="SNR60"
/locus_tag="YNCJ0009C"
/db_xref="GeneID:9164911"
ncRNA complement(349130..349233)
/ncRNA_class="snoRNA"
/gene="SNR60"
/locus_tag="YNCJ0009C"
/product="SNR60"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:10024243]"
/experiment="EXISTENCE:curator inference:GO:0031428 box
C/D methylation guide snoRNP complex [PMID:10024243]"
/experiment="EXISTENCE:mutant phenotype:GO:0030562 rRNA
2'-O-ribose methylation guide activity [PMID:10024243]"
/experiment="EXISTENCE:mutant phenotype:GO:0031167 rRNA
methylation [PMID:10024243]"
/note="C/D box small nucleolar RNA (snoRNA); guides
2'-O-methylation of large subunit (LSU) rRNA at positions
A817 and G908"
/transcript_id="NR_132196.1"
/db_xref="GeneID:9164911"
/db_xref="SGD:S000006451"
gene complement(<349580..>352108)
/gene="RTT101"
/locus_tag="YJL047C"
/gene_synonym="CUL8"
/db_xref="GeneID:853400"
mRNA complement(<349580..>352108)
/gene="RTT101"
/locus_tag="YJL047C"
/gene_synonym="CUL8"
/product="cullin RTT101"
/transcript_id="NM_001181480.3"
/db_xref="GeneID:853400"
CDS complement(349580..352108)
/gene="RTT101"
/locus_tag="YJL047C"
/gene_synonym="CUL8"
/experiment="EXISTENCE:direct assay:GO:0004842
ubiquitin-protein transferase activity [PMID:12676951]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0035361 Cul8-RING
ubiquitin ligase complex [PMID:20139071]"
/experiment="EXISTENCE:mutant phenotype:GO:0007088
regulation of mitotic nuclear division [PMID:12676951]"
/experiment="EXISTENCE:mutant phenotype:GO:0010526
transposable element silencing [PMID:11779788]"
/experiment="EXISTENCE:mutant phenotype:GO:0031297
replication fork processing [PMID:18704118]"
/experiment="EXISTENCE:mutant phenotype:GO:0070651
nonfunctional rRNA decay [PMID:22505030|PMID:19390089]"
/note="Cullin subunit of Roc1p-dependent E3 ubiquitin
ligase complex; role in anaphase progression; functions as
DPC (DNA-protein crosslink) repair factor throughout cell
cycle; Rtt101p-Mms22p ligase associates with replisome
complex during S phase via Ctf4p; required for recovery
after DSB repair; implicated in Mms22-dependent DNA
repair; involved with Mms1p in nonfunctional rRNA decay;
modified by ubiquitin-like protein Rub1p"
/codon_start=1
/product="cullin RTT101"
/protein_id="NP_012488.3"
/db_xref="GeneID:853400"
/db_xref="SGD:S000003583"
/translation="MINESVSKREGFHESISRETSASNALGLYNKFNDERNPRYRTMI
AELHEFFHLTLAETITETDVKELECNKEKAAKFRKLMPKMLNNCRELTQRKSYIPYNS
EFNGNDEKQKKFQLLHQHQIVLSFQEFCDELAKLIIDAHVLSFLTRCDYSYEIIPKNW
TSFYKLFQYVMGAVGPIISYVPVNYPMIRKELGFETLTIFQYYDSKLFECMKSHFGRE
FSTLVSATIHHYIHMFPITNTMLEKEVPMLRIMSNCNFSIEGLSPKDFYMKTLRQYYC
EESNLRPRLETFKNFKVLLTRNALLASLFSPEWVSDANDLFISHLLLNKKSISEYIEI
GKDTYDEEKERYFKTETHFSLLMFRNAFEAKNMLSKFKEFCDDAVSEKLKAAYGSNHD
TERLFDEVVQLANVDHLKIYSDSIEYHLCNLLGSTSKAIEQYVKYFESHLFIIVRKIK
TTKKDLPRDMKIKYLNENLPILRLKFVNLPTFPNFFERSIFRKTILQSDQNSSFIKDI
LPVYKDSLMELFKQRIITNVSQEDEMRYRDQYQPYLSQFFQPVEVMADLRIKYASFLS
FYENIEAAVKFGKTYNENNSKSFFPLIFDRERIPKVFQQSNEVKKNFVLPQEMDDTWN
QFLRNYHEQNKVEDSDASKKELYPMWNLHHCEVESPYIIQDGTNLIFELTLFQTCVLT
LFNESDHLTLQVISEQTKLAYKDLALVLKSFCNYKILTRDIDNTYSINESFKPDMKKV
KNGKLRVVLPRTASLQSSNTGGERTSSAHHEGSNSQWTQELLKACITRSVKSERNGLD
YDHLFETVKQQIKGFSVGEFKDALAKLLRDKFITRDESTATYKY"
gene <352386..>353615
/gene="AIM22"
/locus_tag="YJL046W"
/gene_synonym="LIP3; RRG3"
/db_xref="GeneID:853401"
mRNA <352386..>353615
/gene="AIM22"
/locus_tag="YJL046W"
/gene_synonym="LIP3; RRG3"
/product="putative lipoate--protein ligase"
/transcript_id="NM_001181479.1"
/db_xref="GeneID:853401"
CDS 352386..353615
/gene="AIM22"
/locus_tag="YJL046W"
/gene_synonym="LIP3; RRG3"
/EC_number="6.3.1.20"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:19570983]"
/experiment="EXISTENCE:mutant phenotype:GO:0009249 protein
lipoylation [PMID:19570983]"
/note="Lipoate-protein ligase; octanoyl-CoA: protein
transferase required along with Lip2 and Lip5 for
lipoylation of Lat1p and Kgd2p; similar to E. coli LplA;
null mutant displays reduced frequency of mitochondrial
genome loss"
/codon_start=1
/product="putative lipoate--protein ligase"
/protein_id="NP_012489.2"
/db_xref="GeneID:853401"
/db_xref="SGD:S000003582"
/translation="MSMMLSNWALSPRYVGQRNLIHCTTLFHTLTRWAKDADDKYHDI
NSMYENMFTPSNDNVSILQDEGKSDYDTTKASSMEEDISAFNKDLYNFYNIGYAKQIM
SASQLENIVKAKGRFVIQSLSTSPYYNLALENYVFKNTPRAKRGPDNCRLLFYINDRC
AVIGKNQNLWQEVDLAKLKSKNFELLRRFSGGGTVLHDLGNVNYSYLTSREKFETKFF
NKMIIKWLNSLNPELRLDLNERGDIIQDGFKISGSAYKIAGGKAYHHATMLLNADLEQ
FSGLLEPSLPNNMEWESSGVHSVKSKIKNVGIITPNQFIAVVSERFQKTFKVDGEIPI
YYCDEFKSINDEIKDAMNTLQSEQWKYFSGPKFSVKIKDKGLTIKVEKGMIYDCDRND
LIGLEFKGFLENIDSYT"
gene 354244..354332
/gene="SUP7"
/locus_tag="YNCJ0010W"
/db_xref="GeneID:853402"
tRNA join(354244..354282,354297..354332)
/gene="SUP7"
/locus_tag="YNCJ0010W"
/product="tRNA-Tyr"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Tyrosine tRNA (tRNA-Tyr), predicted by tRNAscan-SE
analysis; can mutate to suppress ochre nonsense mutations"
/db_xref="GeneID:853402"
/db_xref="SGD:S000006781"
repeat_region complement(354538..355204)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007023"
repeat_region complement(354844..355175)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007024"
gene 355374..355445
/locus_tag="YNCJ0011W"
/db_xref="GeneID:853403"
tRNA 355374..355445
/locus_tag="YNCJ0011W"
/product="tRNA-Arg"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE
analysis; one of 11 nuclear tRNA genes containing the
tDNA-anticodon UCU (converted to mcm5-UCU in the mature
tRNA), decodes AGA codons into arginine, one of 19 nuclear
tRNAs for arginine"
/db_xref="GeneID:853403"
/db_xref="SGD:S000006714"
gene 355456..355527
/locus_tag="YNCJ0012W"
/db_xref="GeneID:853404"
tRNA 355456..355527
/locus_tag="YNCJ0012W"
/product="tRNA-Asp"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:853404"
/db_xref="SGD:S000006537"
gene <356024..>357928
/gene="SDH9"
/locus_tag="YJL045W"
/db_xref="GeneID:853405"
mRNA <356024..>357928
/gene="SDH9"
/locus_tag="YJL045W"
/product="succinate dehydrogenase SDH9"
/transcript_id="NM_001181478.1"
/db_xref="GeneID:853405"
CDS 356024..357928
/gene="SDH9"
/locus_tag="YJL045W"
/EC_number="1.3.5.1"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278|PMID:24769239]"
/experiment="EXISTENCE:genetic interaction:GO:0008177
succinate dehydrogenase (quinone) activity
[PMID:9730279|PMID:16232921]"
/experiment="EXISTENCE:genetic interaction:GO:0045333
cellular respiration [PMID:9730279|PMID:16232921]"
/experiment="EXISTENCE:mutant phenotype:GO:0008177
succinate dehydrogenase (quinone) activity
[PMID:16232921]"
/experiment="EXISTENCE:mutant phenotype:GO:0045333
cellular respiration [PMID:16232921]"
/note="Minor succinate dehydrogenase isozyme; participates
in oxidation of succinate and transfer of electrons to
ubiquinone; induced during the diauxic shift in a
Cat8p-dependent manner; YJL045W has a paralog, SDH1, that
arose from the whole genome duplication"
/codon_start=1
/product="succinate dehydrogenase SDH9"
/protein_id="NP_012490.1"
/db_xref="GeneID:853405"
/db_xref="SGD:S000003581"
/translation="MLSLKKGITKSYILQRTFTSSSVVRQIGEVKSESKPPAKYHIID
HEYDCVVVGAGGAGLRAAFGLAEAGYKTACLSKLFPTRSHTVAAQGGINAALGNMHPD
DWKSHMYDTVKGSDWLGDQDAIHYMTREAPKSVIELEHYGMPFSRTEDGRIYQRAFGG
QSKDFGKGGQAYRTCAVADRTGHAMLHTLYGQALKNNTHFFIEYFAMDLLTHNGEVVG
VIAYNQEDGTIHRFRAHKTVIATGGYGRAYFSCTSAHTCTGDGNAMVSRAGFPLEDLE
FVQFHPSGIYGSGCLITEGARGEGGFLLNSEGERFMERYAPTAKDLASRDVVSRAITM
EIRAGRGVGKNKDHILLQLSHLPPEVLKERLPGISETAAVFAGVDVTQEPIPVLPTVH
YNMGGIPTKWTGEALTIDEETGEDKVIPGLMACGEAACVSVHGANRLGANSLLDLVVF
GRAVANTIADTLQPGLPHKPLASNIGHESIANLDKVRNARGSLKTSQIRLNMQRTMQK
DVSVFRTQDTLDEGVRNITEVDKTFEDVHVSDKSMIWNSDLVETLELQNLLTCATQTA
VSASKRKESRGAHAREDYAKRDDVNWRKHTLSWQKGTSTPVKIKYRNVIAHTLDENEC
APVPPAVRSY"
gene complement(<358300..>359676)
/gene="GYP6"
/locus_tag="YJL044C"
/db_xref="GeneID:853406"
mRNA complement(<358300..>359676)
/gene="GYP6"
/locus_tag="YJL044C"
/product="GTPase-activating protein GYP6"
/transcript_id="NM_001181477.3"
/db_xref="GeneID:853406"
CDS complement(358300..359676)
/gene="GYP6"
/locus_tag="YJL044C"
/experiment="EXISTENCE:direct assay:GO:0005096 GTPase
activator activity [PMID:8441469]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:35134079]"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:14610088]"
/experiment="EXISTENCE:direct assay:GO:0005802 trans-Golgi
network [PMID:24924636]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0090630 activation
of GTPase activity [PMID:8441469]"
/note="GTPase-activating protein (GAP) for yeast Rab
family member Ypt6p; involved in vesicle mediated protein
transport"
/codon_start=1
/product="GTPase-activating protein GYP6"
/protein_id="NP_012491.3"
/db_xref="GeneID:853406"
/db_xref="SGD:S000003580"
/translation="MKDVLQWAIDHYETRDQLVQKGIWKGELYHDSIFKENSRGWLWK
ALLLCDENNNCLLTDNFKGLDLNQFGLVPVPMLADGDNYDENHNANVPKRVLHSNVSS
SVGIRRLTPVEAVEKHPLSDDNDKTKGSLSKGSDEKPLTLRETLEIIDLDLSRIMLDD
IFQEPKVHAQMRQLLYNYLLIHQSEHLQYKQGFHEILSVIYLQLYHGTDLDNTDLQNV
LIIFNKLMNQIEPIFYNEENLINWDKRVFTKIFRICLPDLFSKVFYQPPKTGSGKKKN
VDHLIHSNLIWLIRWTRLLFLRELPLKYVLIVWDHVLTFNYPLDIFIACTIITLLLSI
YDELHELVSQGDYEHTNNNDEFVELILHFKKIFEKEDASKDDEKFLDLCKVTGNLCEL
WYGKNYDDMRLICDTFINAKFGIKTSDVLSMETAKLTIDPNRQSLENKLRERVRQTIL
KNKKKISQ"
gene <360130..>360903
/locus_tag="YJL043W"
/db_xref="GeneID:853407"
mRNA <360130..>360903
/locus_tag="YJL043W"
/product="uncharacterized protein"
/transcript_id="NM_001181476.1"
/db_xref="GeneID:853407"
CDS 360130..360903
/locus_tag="YJL043W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095]"
/note="hypothetical protein; YJL043W is a non-essential
gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_012492.1"
/db_xref="GeneID:853407"
/db_xref="SGD:S000003579"
/translation="MFVDYSGLERYTDINASFGKLVNTYCCFQRCEAISEQLEILKSL
VPKCHDIVALTDEDFASGRTAGLTQKLFAMAMTLHQITDCIDLLQKCNTIIPIEIANP
ASFESGAATAPLRQSYARLLDDWSHYMGPSTVKHTGCTNRPKWRFPWQQSRTIIIPML
FIGETAMSTRDLRSVLHDCEIRHASEMPLQLLWTSSPELVYATPHVDDYDIWSRYGSD
YNMQIEDEDEASKGRQRKCVVQLEALLGALPTTDPLFQW"
gene <361249..>365445
/gene="MHP1"
/locus_tag="YJL042W"
/db_xref="GeneID:853408"
mRNA <361249..>365445
/gene="MHP1"
/locus_tag="YJL042W"
/product="Mhp1p"
/transcript_id="NM_001181475.1"
/db_xref="GeneID:853408"
CDS 361249..365445
/gene="MHP1"
/locus_tag="YJL042W"
/experiment="EXISTENCE:direct assay:GO:0005200 structural
constituent of cytoskeleton [PMID:9855114]"
/experiment="EXISTENCE:direct assay:GO:0005874 microtubule
[PMID:8947554]"
/experiment="EXISTENCE:mutant phenotype:GO:0007026
negative regulation of microtubule depolymerization
[PMID:9855114]"
/experiment="EXISTENCE:mutant phenotype:GO:0031505
fungal-type cell wall organization [PMID:9090049]"
/note="Microtubule-associated protein involved in
microtubule organization; involved in assembly and
stabilization of microtubules; overproduction results in
cell cycle arrest at G2 phase; similar to Drosophila
protein MAP and to mammalian MAP4 proteins"
/codon_start=1
/product="Mhp1p"
/protein_id="NP_012493.1"
/db_xref="GeneID:853408"
/db_xref="SGD:S000003578"
/translation="MDSKDTQKLLKEHRIPCIDVGWLVRPSASTSKSSRPGKSESKAN
SVAPDIQMDTARPPVFETSVDSSSSILSSNDKGRRHSVAASLLMDNQRANAGSTSVPT
NIPPPRGRSKSVVETNLSNVEADSGHHHHHRHHHHTEDAPAPKKVGFFKSLFGHRKKD
QEQQEKERERKERSPSPTHVDRGAAIRRERTATISAESPPPLQYNAPPSYNDTVVPLT
RSKTESEVYYENHPQSYYHGRMRTYHSPEEGKVDGTSPADDHNYGGSRPDPRLMDFLR
YYKSKDYKLAAFKEGNFIKSSASPTTKKNRRASFSLHNDKPQPAKSLAHQKFDAKGRP
IPPHPDAPKLPSAFRKKHPSNASIVDTVDSNSDVSSSAQNNNQTPSSHKFGAFLRKVT
SYGNNNNNSTNASSLSANVNNPDTSSTSLWSSSSMEFDPSKITTVPGLENIRPLKHVS
FATNTYFNDPPQQICSKNPRKGEVEVKPNGSVVIHRLTPQERKKIMESTSLGVVVGGT
GQLKLLNPEEDDANAKSKEEMAPQKQNEVEAHDEEDNNSQRRNIVMAAAEAAAEARAK
EAPNELKRIVTNNEEEVTVSKTASHLTIDKPMISRRGASTSSLASMVSSDTNGTNADD
EGEILPPPSLKIPHDIVYTRCCHLREILPIPATLKQLKKGSTDPIPILQLRNPRPSMV
EIWSFSDFLSIAPVLCLSLDGVQLTVQMLRIILSSLVYKQHFQKLSLRNTPLDEEGWK
VLCYFVSKAKSLHSIDLTMVPSIKTNVQKPSKSSLKSKILRMQCNLENRSDMNWDLLT
ASIALMGGLEEIVISGAKMNSAQFKNFILVACIATERLGLAYNGLSKSQCDDLAKWMV
QSKVTGLDVGFNDLNGKLSSFTDAVLGKIQKANEKNVFKFLSLNGTNLRVNEHDTFEN
NEVLKLISVLCYLENLKFLDISNNPAIFPHCVPTLIDFLPVFVNLVRLHIDYNNLSST
SVVMLAEILPMCSRLNYFSMLGTELDLASSKALAEAVRKSSSLMTLDVDYVYMPENIK
EKISLYALRNIQGELKRVNSDDKDIKDSQFSSLQDQLSLLLTEKADNSEHYNKMVENF
MAKIALARIKISKVVHDLFDLKLNGQLNLEGKEALIRLCFIEASLERGCDLLKQRHNN
TLKSPEAVSKSRKGGNQAQPNSESCQRMLLSSSILQNSDHIALMPFGSAIVEKSSPDA
EDAVEFREGDDSNVNHEDVPANDQQFRDEVDIKNKYSIIKRELEHEKLVGGGDLPVDK
EILNRAAQSLDSDQIKEFLLKNDVSTILGVIDELHSQGYHLHHIFKKQGNQEETAFRT
KDEQQSSQSNDSSANASPTTDPISTGSNTSRTNDNAHIPPTDAPGFDKFMNNAEENAI
DAAYDDVLDKIQDARNSSTK"
gene <365784..>368373
/gene="NSP1"
/locus_tag="YJL041W"
/db_xref="GeneID:853409"
mRNA join(<365784,365903..>368373)
/gene="NSP1"
/locus_tag="YJL041W"
/product="FG-nucleoporin NSP1"
/transcript_id="NM_001181474.3"
/db_xref="GeneID:853409"
CDS join(365784,365903..368373)
/gene="NSP1"
/locus_tag="YJL041W"
/experiment="EXISTENCE:direct assay:GO:0005543
phospholipid binding [PMID:17897934]"
/experiment="EXISTENCE:direct assay:GO:0005643 nuclear
pore [PMID:10684247|PMID:2112428]"
/experiment="EXISTENCE:direct assay:GO:0017056 structural
constituent of nuclear pore [PMID:19680227|PMID:19098896]"
/experiment="EXISTENCE:direct assay:GO:0034399 nuclear
periphery [PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0044613 nuclear
pore central transport channel
[PMID:9813081|PMID:24574455|PMID:18046406]"
/experiment="EXISTENCE:direct assay:GO:0044615 nuclear
pore nuclear basket [PMID:9813081]"
/experiment="EXISTENCE:genetic interaction:GO:0006607
NLS-bearing protein import into nucleus [PMID:15039779]"
/experiment="EXISTENCE:genetic interaction:GO:0016973
poly(A)+ mRNA export from nucleus [PMID:10952996]"
/experiment="EXISTENCE:mutant phenotype:GO:0000055
ribosomal large subunit export from nucleus
[PMID:11739405|PMID:11071906|PMID:9971735]"
/experiment="EXISTENCE:mutant phenotype:GO:0000056
ribosomal small subunit export from nucleus
[PMID:11739405]"
/experiment="EXISTENCE:mutant phenotype:GO:0006405 RNA
export from nucleus [PMID:11352936]"
/experiment="EXISTENCE:mutant phenotype:GO:0006409 tRNA
export from nucleus [PMID:8524308]"
/experiment="EXISTENCE:mutant phenotype:GO:0006606 protein
import into nucleus [PMID:12730220|PMID:2112428]"
/experiment="EXISTENCE:physical interaction:GO:0017056
structural constituent of nuclear pore
[PMID:19098896|PMID:12169684|PMID:17418788]"
/note="FG-nucleoporin component of central core of the
nuclear pore complex; also part of the nuclear pore
complex (NPC) nuclear basket; contributes directly to
nucleocytoplasmic transport and maintenance of the NPC
permeability barrier; found in stable complex with Nup82p,
Gle2p and two other FG-nucleoporins (Nup159p and Nup116p);
also found in stable complex with Nic96p and two other
FG-nucleoproteins (Nup49p and Nup57p)"
/codon_start=1
/product="FG-nucleoporin NSP1"
/protein_id="NP_012494.3"
/db_xref="GeneID:853409"
/db_xref="SGD:S000003577"
/translation="MNFNTPQQNKTPFSFGTANNNSNTTNQNSSTGAGAFGTGQSTFG
FNNSAPNNTNNANSSITPAFGSNNTGNTAFGNSNPTSNVFGSNNSTTNTFGSNSAGTS
LFGSSSAQQTKSNGTAGGNTFGSSSLFNNSTNSNTTKPAFGGLNFGGGNNTTPSSTGN
ANTSNNLFGATANANKPAFSFGATTNDDKKTEPDKPAFSFNSSVGNKTDAQAPTTGFS
FGSQLGGNKTVNEAAKPSLSFGSGSAGANPAGASQPEPTTNEPAKPALSFGTATSDNK
TTNTTPSFSFGAKSDENKAGATSKPAFSFGAKPEEKKDDNSSKPAFSFGAKSNEDKQD
GTAKPAFSFGAKPAEKNNNETSKPAFSFGAKSDEKKDGDASKPAFSFGAKPDENKASA
TSKPAFSFGAKPEEKKDDNSSKPAFSFGAKSNEDKQDGTAKPAFSFGAKPAEKNNNET
SKPAFSFGAKSDEKKDGDASKPAFSFGAKSDEKKDSDSSKPAFSFGTKSNEKKDSGSS
KPAFSFGAKPDEKKNDEVSKPAFSFGAKANEKKESDESKSAFSFGSKPTGKEEGDGAK
AAISFGAKPEEQKSSDTSKPAFTFGAQKDNEKKTEESSTGKSTADVKSSDSLKLNSKP
VELKPVSLDNKTLDDLVTKWTNQLTESASHFEQYTKKINSWDQVLVKGGEQISQLYSD
AVMAEHSQNKIDQSLQYIERQQDELENFLDNFETKTEALLSDVVSTSSGAAANNNDQK
RQQAYKTAQTLDENLNSLSSNLSSLIVEINNVSNTFNKTTNIDINNEDENIQLIKILN
SHFDALRSLDDNSTSLEKQINSIKK"
gene complement(<368748..>373799)
/gene="NUP192"
/locus_tag="YJL039C"
/db_xref="GeneID:853410"
mRNA complement(<368748..>373799)
/gene="NUP192"
/locus_tag="YJL039C"
/product="Nup192p"
/transcript_id="NM_001181473.1"
/db_xref="GeneID:853410"
CDS complement(368748..373799)
/gene="NUP192"
/locus_tag="YJL039C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005643 nuclear
pore [PMID:10684247]"
/experiment="EXISTENCE:direct assay:GO:0044611 nuclear
pore inner ring [PMID:18046406]"
/experiment="EXISTENCE:mutant phenotype:GO:0006999 nuclear
pore organization [PMID:10428845|PMID:11121302]"
/experiment="EXISTENCE:mutant phenotype:GO:0017056
structural constituent of nuclear pore [PMID:10428845]"
/experiment="EXISTENCE:mutant phenotype:GO:0046822
regulation of nucleocytoplasmic transport [PMID:23499021]"
/note="Essential subunit of inner ring of nuclear pore
complex (NPC); plays a role in modulating transport
through the NPC; homologous to human NUP205"
/codon_start=1
/product="Nup192p"
/protein_id="NP_012495.1"
/db_xref="GeneID:853410"
/db_xref="SGD:S000003576"
/translation="MKWSAIPFQTLYRSIESGEFDFDLFKEVLPDLQNLNLNTDKLKN
NASRSQLEKGEIELSDGSTFKVNQEFIFEAISLSDELNLDEIVACELILSGDTTANNG
KVQYFLRRQYILQIVSFIVNCFHEDTELYQELIKNGALVSNILSAFKFIHTQLSEIKQ
QINKAQILENYNALFQQNIKFRRDFLLREYDILSQILYGLVDKGAIMKNKDFILSLLH
HVSELDSNDFFIIYYTPAFFHLFASLRVLPDADVKLLHSQFMKDLKDDSIYTKPVKVA
LIFIFFAYFIGWCKEDPKRRADTMDFKTDVDEPMTSAVELGAIEQILIFAADTSIVEQ
DKSMELFYDIRSLLERHIPRLIPKQLLDDEKIFSQTTNSTYNPASATDNMSGRGLWNP
SYPGMMSTTGTARLNSMPNNVNEYSYTTIVLSDQTQEFFLSSFDDVLQTIITDCAFLL
TKIKDAEEDSLLSGEDLTLDDISLKADLERFFLSIYFFYASRPEYSCTFWSDKESNAY
GFIEWCSRCNDNLMRSCFYLMVSSLSFGPENALNVYHYFGENSSISWKNIAQCLSDYT
KKISNFNSSLHKRQQFSESTHNDIDSTAVALEEGLNEEAVIFLSSLLTLVGSVTYQVD
EDVKSSLSKVFSDVLFEFTKINTPLVGAAFKVISNLVPKLESSRTKFWSFLDSLIFKD
SSLNYSSESYRNAFTNVLTKYSDVLGFLQLFHNLISIHSRENNSEYMVFGKLAFPTRL
GQGYRKVGIWPYFDYIFNDILAHVDQIVDIRNKRAVQLPILKIIYTGLCSFDYSVILN
SIPAAANLDALVDCENFFNYVQECPAIPIFNYIFTEKIYKSIFNVVDVGVDQLSIELE
GGKNQAELLQLAVKIINKVLDYQETYVEELFPIVKKHGKTDYFLPKNYSLHGLRSFYD
AIFFNIPLVAHLGLYVGVDDQILATNSLRILAKLSERSNGSVASLSKRNKLLTIFDSV
DESARIKDAFITQLESSITDAGVLALKLELLDFLTSNLSNYSRTMTISHLLLGFQVSN
VISLGPNLATFISSGTSLLDSLISVLEASLNSITKDNIDYAPMRLATAALEIILKLCR
NPLTSGLLYSYLIKENFFERIMILDPQVTRFTTWNGSPFDNSTEEKCKNFIESESVGA
FLSFLAYRNYWTQYLGLFIHKISFSGTKSEVLTYVNYLISNTMYSVRLFSFLDPLNYG
NICEPKETLSIFTNVPLNLEQVTLNKYCSGNIYDFHKMENLMRLIKRVRAESLHSNSF
SLTVSKEQFLKDADVECIKAKSHFTNIISRNKALELNLSVLHSWVQLVQIIVTDGKLE
PSTRSNFILEVFGTIIPKISDYIEFNITFSEELVSLAVFLFDIYNRDRKLITDKGTVD
GRLYQLFKTCIQGINSPLSSVALRSDFYILANHYLSRVLSDQVGSEKVLQDLRLGSKK
LVEIIWNDVVYGEGTSRVTGILLLDSLIQLANRSKENFILDSLMKTTRLLLIIRSLKN
TDALLNSTTEHINIDDLLYELTAFKATVFFLIRVAETRGGASALIENNLFRIIAELSF
LKVDPDLGLDLMFDEVYVQNSKFLKVNVTLDNPLLVDKDANGVSLFELIVPIFQLISA
VLVSMGSSNKAVVQTVKGLLNTYKRLVIGIFKRDLLREKEDKKNSSDPNNQSLNEMVK
LIVMLCTLTGYQNND"
gene complement(374424..374495)
/locus_tag="YNCJ0013C"
/db_xref="GeneID:853411"
tRNA complement(374424..374495)
/locus_tag="YNCJ0013C"
/product="tRNA-Asp"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:853411"
/db_xref="SGD:S000006538"
gene complement(374506..374577)
/locus_tag="YNCJ0014C"
/db_xref="GeneID:853412"
tRNA complement(374506..374577)
/locus_tag="YNCJ0014C"
/product="tRNA-Arg"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE
analysis; one of 11 nuclear tRNA genes containing the
tDNA-anticodon UCU (converted to mcm5-UCU in the mature
tRNA), decodes AGA codons into arginine, one of 19 nuclear
tRNAs for arginine"
/db_xref="GeneID:853412"
/db_xref="SGD:S000006715"
repeat_region complement(374844..374935)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007025"
rep_origin 374880..375122
/note="ARS1012; Autonomously Replicating Sequence"
/db_xref="SGD:S000028449"
gene complement(<375115..>375774)
/gene="LOH1"
/locus_tag="YJL038C"
/gene_synonym="OSW4"
/db_xref="GeneID:853413"
mRNA complement(<375115..>375774)
/gene="LOH1"
/locus_tag="YJL038C"
/gene_synonym="OSW4"
/product="Loh1p"
/transcript_id="NM_001181472.1"
/db_xref="GeneID:853413"
CDS complement(375115..375774)
/gene="LOH1"
/locus_tag="YJL038C"
/gene_synonym="OSW4"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0030476
ascospore wall assembly [PMID:23966878]"
/experiment="EXISTENCE:mutant phenotype:GO:0051276
chromosome organization [PMID:18562670]"
/note="Protein involved in outer spore wall assembly;
likely involved directly in dityrosine layer assembly;
induced during sporulation; repressed during vegetative
growth by Sum1p and Hst1p; sequence similar to adjacent
ORF, IRC18/YJL037W, and the irc18 loh1 double mutant
exhibits reduced dityrosine fluorescence relative to the
single mutants; SWAT-GFP and mCherry fusion proteins
localize to the cytosol; proposed role in maintenance of
genome integrity"
/codon_start=1
/product="Loh1p"
/protein_id="NP_012496.1"
/db_xref="GeneID:853413"
/db_xref="SGD:S000003575"
/translation="MRFQLFIYFYFTIVVIAGTNTIQQFSDAGDRLITSLRNLDNNGT
YETLTAEKVPIIEGQIQNISAKYEQHTFILKGLEAVLNYKVKSLDNNERESLEIEYEK
VEKALDAALNVSPFEYIKKFKEVSRGKVVNALENLSREQNRITINGGREDEKEKEARE
KKKRLDRIKRILTVSLLELGLAQGVADLCAVAPFACLLGVTVGSIGFIFWLALIYNAI
Q"
rep_origin 375706..376227
/note="ARS1013; Autonomously Replicating Sequence;
inefficient as chromosomal replication origin; replication
capacity dependent upon Sum1p"
/db_xref="SGD:S000028450"
gene <376662..>377336
/gene="IRC18"
/locus_tag="YJL037W"
/gene_synonym="OSW6"
/db_xref="GeneID:853414"
mRNA <376662..>377336
/gene="IRC18"
/locus_tag="YJL037W"
/gene_synonym="OSW6"
/product="Irc18p"
/transcript_id="NM_001181471.3"
/db_xref="GeneID:853414"
CDS 376662..377336
/gene="IRC18"
/locus_tag="YJL037W"
/gene_synonym="OSW6"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0030476
ascospore wall assembly [PMID:23966878]"
/note="Protein involved in outer spore wall assembly;
possible role in assembly of the dityrosine layer; similar
to adjacent ORF, LOH1; irc18 loh1 double mutant exhibits
reduced dityrosine fluorescence relative to single
mutants; SWAT-GFP fusion protein localizes to the ER and
vacuole, while mCherry fusion localizes to the vacuole;
expression induced in respiratory-deficient cells and
carbon-limited chemostat culture; null mutant displays
increased levels of spontaneous Rad52p foci"
/codon_start=1
/product="Irc18p"
/protein_id="NP_012497.3"
/db_xref="GeneID:853414"
/db_xref="SGD:S000003574"
/translation="MKVQMIERIFLIQLCLLTVVLASSRAVVEFESTGTKLVNSLRVL
AAYSQSSVCVDEKISGIERQIEEVKDMYGNHSFILKGLNGILNNKVNMLTREIQMETV
GNNTFETETGKLTKGLNRAVNISPFKYIKKFKTVSTKKFESLLNKYDLVAKKGGELTE
EQKKKKEVLSRISRVVAATTIEAGLAQGVVDLCITVTTSLCLVSASIGGVGFLIWLTI
IYQALT"
repeat_region complement(377598..377870)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007026"
gene 378360..378433
/locus_tag="YNCJ0015W"
/db_xref="GeneID:853415"
tRNA 378360..378433
/locus_tag="YNCJ0015W"
/product="tRNA-Val"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Valine tRNA (tRNA-Val), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:853415"
/db_xref="SGD:S000006759"
gene <378825..>380096
/gene="SNX4"
/locus_tag="YJL036W"
/gene_synonym="ATG24; CVT13"
/db_xref="GeneID:853416"
mRNA <378825..>380096
/gene="SNX4"
/locus_tag="YJL036W"
/gene_synonym="ATG24; CVT13"
/product="Snx4p"
/transcript_id="NM_001181470.1"
/db_xref="GeneID:853416"
CDS 378825..380096
/gene="SNX4"
/locus_tag="YJL036W"
/gene_synonym="ATG24; CVT13"
/experiment="EXISTENCE:direct assay:GO:0000407 phagophore
assembly site [PMID:12048214|PMID:18497569]"
/experiment="EXISTENCE:direct assay:GO:0005769 early
endosome [PMID:17420293]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762|PMID:12048214]"
/experiment="EXISTENCE:direct assay:GO:0032266
phosphatidylinositol-3-phosphate binding
[PMID:12554655|PMID:11557775]"
/experiment="EXISTENCE:direct assay:GO:0048471 perinuclear
region of cytoplasm [PMID:33678121]"
/experiment="EXISTENCE:genetic interaction:GO:0034498
early endosome to Golgi transport [PMID:12554655]"
/experiment="EXISTENCE:genetic interaction:GO:0036010
protein localization to endosome [PMID:29511122]"
/experiment="EXISTENCE:mutant phenotype:GO:0000422
autophagy of mitochondrion [PMID:19793921]"
/experiment="EXISTENCE:mutant phenotype:GO:0006623 protein
targeting to vacuole [PMID:12554655]"
/experiment="EXISTENCE:mutant phenotype:GO:0032258
cytoplasm to vacuole targeting by the Cvt pathway
[PMID:12048214|PMID:12554655]"
/experiment="EXISTENCE:mutant phenotype:GO:0032456
endocytic recycling [PMID:32074001]"
/experiment="EXISTENCE:mutant phenotype:GO:0034498 early
endosome to Golgi transport [PMID:12554655]"
/experiment="EXISTENCE:mutant phenotype:GO:0034727
piecemeal microautophagy of the nucleus [PMID:18701704]"
/experiment="EXISTENCE:mutant phenotype:GO:0061723
glycophagy [PMID:38832010]"
/experiment="EXISTENCE:physical interaction:GO:0034498
early endosome to Golgi transport [PMID:12554655]"
/note="Sorting nexin; involved in retrieval of late-Golgi
SNAREs from post-Golgi endosomes to the trans-Golgi
network and in cytoplasm to vacuole transport; contains a
PX phosphoinositide-binding domain; forms complexes with
Snx41p and with Atg20p"
/codon_start=1
/product="Snx4p"
/protein_id="NP_012498.1"
/db_xref="GeneID:853416"
/db_xref="SGD:S000003573"
/translation="MTDKGKNDLTSKAKDKARGNPEKPPYWFEIIVSDPQKRTGDPGS
SSGYVSYQISTKTNNTSFYDNRGDPESIIVVHRRYSDLLLLHDILLNRFPTCIIPPLP
DKKVFQYIAGDRFSQRFTQKRCHSLQNFLRRVSLHPDLSQSKVFKTFLVSKDWESHRK
VLQDSLQPNKDEVTDAFMNAFKTVHKQNEEFTEIREKSDKLDRTVTKIDKLFHKVVKK
NDSMSEDYTKLGSNLQELQELVTGENEELAAKLKIFNEGVTQLSYGLQDLTKYLDYEY
IVDLKDLEHYIDSMRQLIKLKDQKQIDYEELSDYLTRSIKEKNNLISGYGGSNFFANK
LEELAGINQEASRREKINKLEGKITSLTGELENAKKVADGFEQECLKEIDHFESVKTA
EIKKSLGSLADHHIEFYERILEAWEKVDDSL"
gene complement(<380249..>381001)
/gene="TAD2"
/locus_tag="YJL035C"
/db_xref="GeneID:853417"
mRNA complement(<380249..>381001)
/gene="TAD2"
/locus_tag="YJL035C"
/product="tRNA(adenine34) deaminase"
/transcript_id="NM_001181469.1"
/db_xref="GeneID:853417"
CDS complement(380249..381001)
/gene="TAD2"
/locus_tag="YJL035C"
/EC_number="3.5.4.33"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006400 tRNA
modification [PMID:10550050]"
/experiment="EXISTENCE:direct assay:GO:0008251
tRNA-specific adenosine deaminase activity
[PMID:10550050]"
/note="Subunit of tRNA-specific adenosine-34 deaminase;
forms a heterodimer with Tad3p that converts adenosine to
inosine at the wobble position of several tRNAs"
/codon_start=1
/product="tRNA(adenine34) deaminase"
/protein_id="NP_012499.1"
/db_xref="GeneID:853417"
/db_xref="SGD:S000003572"
/translation="MQHIKHMRTAVRLARYALDHDETPVACIFVHTPTGQVMAYGMND
TNKSLTGVAHAEFMGIDQIKAMLGSRGVVDVFKDITLYVTVEPCIMCASALKQLDIGK
VVFGCGNERFGGNGTVLSVNHDTCTLVPKNNSAAGYESIPGILRKEAIMLLRYFYVRQ
NERAPKPRSKSDRVLDKNTFPPMEWSKYLNEEAFIETFGDDYRTCFANKVDLSSNSVD
WDLIDSHQDNIIQELEEQCKMFKFNVHKKSKV"
gene <381327..>383375
/gene="KAR2"
/locus_tag="YJL034W"
/gene_synonym="GRP78"
/db_xref="GeneID:853418"
mRNA <381327..>383375
/gene="KAR2"
/locus_tag="YJL034W"
/gene_synonym="GRP78"
/product="Hsp70 family ATPase KAR2"
/transcript_id="NM_001181468.3"
/db_xref="GeneID:853418"
CDS 381327..383375
/gene="KAR2"
/locus_tag="YJL034W"
/gene_synonym="GRP78"
/EC_number="3.6.4.10"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:2661018]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:1325440]"
/experiment="EXISTENCE:direct assay:GO:0031204
post-translational protein targeting to membrane,
translocation [PMID:10367885]"
/experiment="EXISTENCE:direct assay:GO:0034099 luminal
surveillance complex [PMID:16873065]"
/experiment="EXISTENCE:direct assay:GO:0051082 unfolded
protein binding [PMID:23704572]"
/experiment="EXISTENCE:genetic interaction:GO:0070880
fungal-type cell wall beta-glucan biosynthetic process
[PMID:9430631]"
/experiment="EXISTENCE:mutant phenotype:GO:0000742
karyogamy involved in conjugation with cellular fusion
[PMID:10069807]"
/experiment="EXISTENCE:mutant phenotype:GO:0006616
SRP-dependent cotranslational protein targeting to
membrane, translocation [PMID:7568189]"
/experiment="EXISTENCE:mutant phenotype:GO:0006986
response to unfolded protein [PMID:12808051]"
/experiment="EXISTENCE:mutant phenotype:GO:0031204
post-translational protein targeting to membrane,
translocation [PMID:7568189]"
/experiment="EXISTENCE:mutant phenotype:GO:0036503 ERAD
pathway [PMID:11381090]"
/experiment="EXISTENCE:mutant phenotype:GO:0051082
unfolded protein binding [PMID:12808051]"
/experiment="EXISTENCE:mutant phenotype:GO:0070972 protein
localization to endoplasmic reticulum [PMID:1996357]"
/note="ATPase involved in protein import into the ER; also
acts as a chaperone to mediate protein folding in the ER
and may play a role in ER export of soluble proteins;
regulates the unfolded protein response via interaction
with Ire1p"
/codon_start=1
/product="Hsp70 family ATPase KAR2"
/protein_id="NP_012500.3"
/db_xref="GeneID:853418"
/db_xref="SGD:S000003571"
/translation="MFFNRLSAGKLLVPLSVVLYALFVVILPLQNSFHSSNVLVRGAD
DVENYGTVIGIDLGTTYSCVAVMKNGKTEILANEQGNRITPSYVAFTDDERLIGDAAK
NQVAANPQNTIFDIKRLIGLKYNDRSVQKDIKHLPFNVVNKDGKPAVEVSVKGEKKVF
TPEEISGMILGKMKQIAEDYLGTKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVLRI
VNEPTAAAIAYGLDKSDKEHQIIVYDLGGGTFDVSLLSIENGVFEVQATSGDTHLGGE
DFDYKIVRQLIKAFKKKHGIDVSDNNKALAKLKREAEKAKRALSSQMSTRIEIDSFVD
GIDLSETLTRAKFEELNLDLFKKTLKPVEKVLQDSGLEKKDVDDIVLVGGSTRIPKVQ
QLLESYFDGKKASKGINPDEAVAYGAAVQAGVLSGEEGVEDIVLLDVNALTLGIETTG
GVMTPLIKRNTAIPTKKSQIFSTAVDNQPTVMIKVYEGERAMSKDNNLLGKFELTGIP
PAPRGVPQIEVTFALDANGILKVSATDKGTGKSESITITNDKGRLTQEEIDRMVEEAE
KFASEDASIKAKVESRNKLENYAHSLKNQVNGDLGEKLEEEDKETLLDAANDVLEWLD
DNFETAIAEDFDEKFESLSKVAYPITSKLYGGADGSGAADYDDEDEDDDGDYFEHDEL
"
gene <383837..>386149
/gene="HCA4"
/locus_tag="YJL033W"
/gene_synonym="DBP4; ECM24"
/db_xref="GeneID:853419"
mRNA <383837..>386149
/gene="HCA4"
/locus_tag="YJL033W"
/gene_synonym="DBP4; ECM24"
/product="RNA-dependent ATPase HCA4"
/transcript_id="NM_001181467.1"
/db_xref="GeneID:853419"
CDS 383837..386149
/gene="HCA4"
/locus_tag="YJL033W"
/gene_synonym="DBP4; ECM24"
/EC_number="3.6.4.13"
/experiment="EXISTENCE:direct assay:GO:0003724 RNA
helicase activity [PMID:23153376]"
/experiment="EXISTENCE:direct assay:GO:0008186
ATP-dependent activity, acting on RNA
[PMID:23153376|PMID:18975973]"
/experiment="EXISTENCE:mutant phenotype:GO:0003724 RNA
helicase activity [PMID:16209945]"
/experiment="EXISTENCE:mutant phenotype:GO:0006364 rRNA
processing [PMID:16209945]"
/experiment="EXISTENCE:mutant phenotype:GO:0032040
small-subunit processome [PMID:24357410]"
/note="DEAD box RNA helicase; component of the SSU;
interacts with Bfr2p and Enp2p; high-copy number
suppression of a U14 snoRNA processing mutant suggests an
involvement in 18S rRNA synthesis"
/codon_start=1
/product="RNA-dependent ATPase HCA4"
/protein_id="NP_012501.1"
/db_xref="GeneID:853419"
/db_xref="SGD:S000003570"
/translation="MAKKNRLNTTQRKTLRQKEDEYIENLKTKIDEYDPKITKAKFFK
DLPISDPTLKGLRESSFIKLTEIQADSIPVSLQGHDVLAAAKTGSGKTLAFLVPVIEK
LYREKWTEFDGLGALIISPTRELAMQIYEVLTKIGSHTSFSAGLVIGGKDVKFELERI
SRINILIGTPGRILQHLDQAVGLNTSNLQMLVLDEADRCLDMGFKKTLDAIVSTLSPS
RQTLLFSATQSQSVADLARLSLTDYKTVGTHDVMDGSVNKEASTPETLQQFYIEVPLA
DKLDILFSFIKSHLKCKMIVFLSSSKQVHFVYETFRKMQPGISLMHLHGRQKQRARTE
TLDKFNRAQQVCLFATDVVARGIDFPAVDWVVQVDCPEDVDTYIHRVGRCARYGKKGK
SLIMLTPQEQEAFLKRLNARKIEPGKLNIKQSKKKSIKPQLQSLLFKDPELKYLGQKA
FISYVRSIYVQKDKQVFKFDELPTEEFAYSLGLPGAPKIKMKGMKTIEQAKERKNAPR
QLAFLSKANEDGEVIEDKSKQPRTKYDKMFERKNQTILSEHYLNITKAQAQEDEDDDF
ISVKRKDHEINEAELPALTLPTSRRAQKKALSKKASLASKGNASKLIFDDEGEAHPVY
ELEDEEEFHKRGDAEVQKTEFLTKESAVMADIDNIDKQVAKEKKQEKKRKRLEAMRRE
MEAAMEEEISGDEEEGKTVAYLGTGNLSDDMSDGDMPDSEGHLKKKARTVDYSHGHNP
SNSVDDDIIEVEEPQTLEDLESLTAKLIQG"
gene complement(<386368..>387438)
/gene="BET4"
/locus_tag="YJL031C"
/db_xref="GeneID:853421"
mRNA complement(join(<386368..387348,387436..>387438))
/gene="BET4"
/locus_tag="YJL031C"
/product="Rab geranylgeranyltransferase BET4"
/transcript_id="NM_001181465.1"
/db_xref="GeneID:853421"
CDS complement(join(386368..387348,387436..387438))
/gene="BET4"
/locus_tag="YJL031C"
/EC_number="2.5.1.60"
/experiment="EXISTENCE:direct assay:GO:0004663 Rab
geranylgeranyltransferase activity [PMID:8756702]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:27663510]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005968 Rab-protein
geranylgeranyltransferase complex [PMID:8756702]"
/experiment="EXISTENCE:direct assay:GO:0018344 protein
geranylgeranylation [PMID:8756702]"
/experiment="EXISTENCE:mutant phenotype:GO:0006612 protein
targeting to membrane [PMID:8232541]"
/experiment="EXISTENCE:mutant phenotype:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:8232541]"
/experiment="EXISTENCE:mutant phenotype:GO:0018344 protein
geranylgeranylation [PMID:8232541]"
/experiment="EXISTENCE:physical interaction:GO:0005968
Rab-protein geranylgeranyltransferase complex
[PMID:8232542]"
/note="Alpha subunit of Type II geranylgeranyltransferase;
required for vesicular transport between the endoplasmic
reticulum and the Golgi; provides a membrane attachment
moiety to Rab-like proteins Ypt1p and Sec4p"
/codon_start=1
/product="Rab geranylgeranyltransferase BET4"
/protein_id="NP_012503.2"
/db_xref="GeneID:853421"
/db_xref="SGD:S000003568"
/translation="MHGIKRKQWTKELLRQKRVQDEKKIYDYRSLTENVLNMRDEKIY
SIEALKKTSELLEKNPEFNAIWNYRRDIIASLASELEIPFWDKELVFVMMLLKDYPKV
YWIWNHRLWVLKHYPTSSPKVWQTELAVVNKLLEQDARNYHGWHYRRIVVGNIESITN
KSLDKEEFEYTTIKINNNISNYSAWHQRVQIISRMFQKGEVGNQKEYIRTEISYIINA
MFTDAEDQSVWFYIKWFIKNDIVCKTLDEQEYLKMLKDLRENILLINNDEIEFSGKQN
IWCLKILLVLEDILEEKEALTERSSEQYLVQLIDADPLRKNRYLHLLEQHK"
gene <387657..>388247
/gene="MAD2"
/locus_tag="YJL030W"
/db_xref="GeneID:853422"
mRNA <387657..>388247
/gene="MAD2"
/locus_tag="YJL030W"
/product="spindle checkpoint protein MAD2"
/transcript_id="NM_001181464.3"
/db_xref="GeneID:853422"
CDS 387657..388247
/gene="MAD2"
/locus_tag="YJL030W"
/experiment="EXISTENCE:direct assay:GO:0000776 kinetochore
[PMID:27170178]"
/experiment="EXISTENCE:direct assay:GO:0033597 mitotic
checkpoint complex [PMID:11726501]"
/experiment="EXISTENCE:direct assay:GO:1902499 positive
regulation of protein autoubiquitination [PMID:22940250]"
/experiment="EXISTENCE:genetic interaction:GO:0044774
mitotic DNA integrity checkpoint signaling
[PMID:16651657]"
/experiment="EXISTENCE:mutant phenotype:GO:0007094 mitotic
spindle assembly checkpoint signaling [PMID:10704439]"
/experiment="EXISTENCE:physical interaction:GO:0007094
mitotic spindle assembly checkpoint signaling
[PMID:9461437]"
/experiment="EXISTENCE:physical interaction:GO:0033597
mitotic checkpoint complex [PMID:11726501|PMID:10704439]"
/note="Component of the spindle-assembly checkpoint
complex; delays onset of anaphase in cells with defects in
mitotic spindle assembly; forms a complex with Mad1p;
regulates APC/C activity during prometaphase and metaphase
of meiosis I; gene dosage imbalance between MAD1 and MAD2
leads to chromosome instability"
/codon_start=1
/product="spindle checkpoint protein MAD2"
/protein_id="NP_012504.3"
/db_xref="GeneID:853422"
/db_xref="SGD:S000003567"
/translation="MSQSISLKGSTRTVTEFFEYSINSILYQRGVYPAEDFVTVKKYD
LTLLKTHDDELKDYIRKILLQVHRWLLGGKCNQLVLCIVDKDEGEVVERWSFNVQHIS
GNSNGQDDVVDLNTTQSQIRALIRQITSSVTFLPELTKEGGYTFTVLAYTDADAKVPL
EWADSNSKEIPDGEVVQFKTFSTNDHKVGAQVSYKY"
gene complement(<388385..>390853)
/gene="VPS53"
/locus_tag="YJL029C"
/db_xref="GeneID:853423"
mRNA complement(<388385..>390853)
/gene="VPS53"
/locus_tag="YJL029C"
/product="Vps53p"
/transcript_id="NM_001181463.1"
/db_xref="GeneID:853423"
CDS complement(388385..390853)
/gene="VPS53"
/locus_tag="YJL029C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:10637310]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0042147 retrograde
transport, endosome to Golgi [PMID:12686613]"
/experiment="EXISTENCE:mutant phenotype:GO:0006896 Golgi
to vacuole transport [PMID:10637310]"
/experiment="EXISTENCE:mutant phenotype:GO:0090156
intracellular sphingolipid homeostasis [PMID:26357016]"
/experiment="EXISTENCE:physical interaction:GO:0000938
GARP complex [PMID:10637310]"
/note="Component of the GARP (Golgi-associated retrograde
protein) complex; GARP is required for the recycling of
proteins from endosomes to the late Golgi, and for mitosis
after DNA damage induced checkpoint arrest; required for
vacuolar protein sorting; members of the GARP complex are
Vps51p-Vps52p-Vps53p-Vps54p; human ortholog is implicated
in progressive cerebello-cerebral atrophy type 2 (PCCA2)"
/codon_start=1
/product="Vps53p"
/protein_id="NP_012505.1"
/db_xref="GeneID:853423"
/db_xref="SGD:S000003566"
/translation="MLEGTVDYDPLEDITNILFSKESLNNIDELISITRSYKKQLQED
ILKEENELKEHPKNSAEIEASLRKVFQDFKETQDVSASTELTISNLTEGISYLDIAKK
NLTHSLTLFQNLKILTDSYIQCNELLSQGSFKKMVSPYKIMCSLAENTFISYKSLDEI
NYLLSSISRLKGDTLSKIKQNYNALFSGGNISEHDTALTMELREGACELLDCDTSTRA
QMIDWCLDKLLFEMKEIFRVDDEAGSLENLSRRYIYFKKILNNFNSKFADYFLKDWEM
AVRLTTTFYHITHKDLQTLLKREFKDKNPSIDLFMTALQSTLDFEKYIDVRFSKKIKE
PKLSSCFEPYLTLWVSHQNQMMEKKFLSYMSEPKYPSNETESLVLPSSADLFRTYRSV
LTQTLELIDNNANDSILTSLANFFSRWLQTYSQKILLPLLLPDNIEVQDKLEAAKYTV
LLINTADYCATTIDQLEDKLSEFSGNREKLANSFTKTKNIYDDLLAKGTSFLLNRVIP
LDLNFVWREFINNDWSNAAIEDYSRYMVTLKSVLKMPALTDASIKQQQEQPSTLAFIL
SQFNRDVYKWNFLDKVIDIITTNFVSNTIRLLQPVPPFSLAGSKRKFETRTVVNIGEQ
LLLDLELLKEIFHTLPESVSNDSDLRENTSYKRVKRHADNNIDQLLKFIKLLMAPLDS
ADDYYETYSKLTNNNPDSAVWSFVLALKGIPWDLALWKKLWSAYNLETDDTDEGSRPD
SNRDLFIFKWDKVLLGQFENNLARMQDPNWSKFVRQDLKISPPVMKRIVSTPQIQQQK
EEQKKQSLSVKDFVSHSRFFNRGT"
gene complement(391043..391115)
/gene="EMT5"
/locus_tag="YNCJ0016C"
/db_xref="GeneID:853424"
tRNA complement(391043..391115)
/gene="EMT5"
/locus_tag="YNCJ0016C"
/product="tRNA-Met"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Methionine tRNA (tRNA-Met), functions in
translational elongation; not involved in translational
initiation; predicted by tRNAscan-SE analysis"
/db_xref="GeneID:853424"
/db_xref="SGD:S000006661"
gene <391311..>391646
/locus_tag="YJL028W"
/db_xref="GeneID:853425"
mRNA <391311..>391646
/locus_tag="YJL028W"
/product="uncharacterized protein"
/transcript_id="NM_001181462.1"
/db_xref="GeneID:853425"
CDS 391311..391646
/locus_tag="YJL028W"
/note="hypothetical protein; may interact with ribosomes,
based on co-purification experiments"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_012506.1"
/db_xref="GeneID:853425"
/db_xref="SGD:S000003565"
/translation="MALWGRSAYRQKTVTSRLTKHRHTSPLNLLNFFIFFSLHLCALF
LATAVHYACFACFVLFRHAILLLFYLLARGRASQIQARQKVRCTGATFYRFLIISLSQ
RAWATKKPI"
gene complement(<391833..>392249)
/locus_tag="YJL027C"
/db_xref="GeneID:853426"
mRNA complement(<391833..>392249)
/locus_tag="YJL027C"
/product="uncharacterized protein"
/transcript_id="NM_001181461.3"
/db_xref="GeneID:853426"
CDS complement(391833..392249)
/locus_tag="YJL027C"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_012507.3"
/db_xref="GeneID:853426"
/db_xref="SGD:S000003564"
/translation="MMVTRKHYRYIYLQNSHSLISCFVHFEFPRVWYGAICPCFPSFA
LLRKIFFCQQQQHATLCAVLRSGLCGNGDIVPMPARREVWVWGVCDLVAMAIARGCGL
SPNGCPLLRISHSCRVNKKHERGRTALNSGRSRDVK"
gene <392404..>393603
/gene="RNR2"
/locus_tag="YJL026W"
/gene_synonym="CRT6"
/db_xref="GeneID:853427"
mRNA <392404..>393603
/gene="RNR2"
/locus_tag="YJL026W"
/gene_synonym="CRT6"
/product="ribonucleotide-diphosphate reductase subunit
RNR2"
/transcript_id="NM_001181460.1"
/db_xref="GeneID:853427"
CDS 392404..393603
/gene="RNR2"
/locus_tag="YJL026W"
/gene_synonym="CRT6"
/EC_number="1.17.4.1"
/experiment="EXISTENCE:direct assay:GO:0004748
ribonucleoside-diphosphate reductase activity, thioredoxin
disulfide as acceptor [PMID:10535923|PMID:10716984]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12732713]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:9315670|PMID:12732713]"
/experiment="EXISTENCE:direct assay:GO:0005971
ribonucleoside-diphosphate reductase complex
[PMID:10716984]"
/experiment="EXISTENCE:direct assay:GO:0009263
deoxyribonucleotide biosynthetic process
[PMID:5459124|PMID:10535923]"
/note="Ribonucleotide-diphosphate reductase (RNR), small
subunit; the RNR complex catalyzes the rate-limiting step
in dNTP synthesis and is regulated by DNA replication and
DNA damage checkpoint pathways via localization of the
small subunits; RNR2 has a paralog, RNR4, that arose from
the whole genome duplication"
/codon_start=1
/product="ribonucleotide-diphosphate reductase subunit
RNR2"
/protein_id="NP_012508.1"
/db_xref="GeneID:853427"
/db_xref="SGD:S000003563"
/translation="MPKETPSKAAADALSDLEIKDSKSNLNKELETLREENRVKSDML
KEKLSKDAENHKAYLKSHQVHRHKLKEMEKEEPLLNEDKERTVLFPIKYHEIWQAYKR
AEASFWTAEEIDLSKDIHDWNNRMNENERFFISRVLAFFAASDGIVNENLVENFSTEV
QIPEAKSFYGFQIMIENIHSETYSLLIDTYIKDPKESEFLFNAIHTIPEIGEKAEWAL
RWIQDADALFGERLVAFASIEGVFFSGSFASIFWLKKRGMMPGLTFSNELICRDEGLH
TDFACLLFAHLKNKPDPAIVEKIVTEAVEIEQRYFLDALPVALLGMNADLMNQYVEFV
ADRLLVAFGNKKYYKVENPFDFMENISLAGKTNFFEKRVSDYQKAGVMSKSTKQEAGA
FTFNEDF"
gene <393967..>395511
/gene="RRN7"
/locus_tag="YJL025W"
/db_xref="GeneID:853428"
mRNA <393967..>395511
/gene="RRN7"
/locus_tag="YJL025W"
/product="Rrn7p"
/transcript_id="NM_001181459.1"
/db_xref="GeneID:853428"
CDS 393967..395511
/gene="RRN7"
/locus_tag="YJL025W"
/experiment="EXISTENCE:direct assay:GO:0001164 RNA
polymerase I core promoter sequence-specific DNA binding
[PMID:18451108]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:22362748]"
/experiment="EXISTENCE:direct assay:GO:0017025 TBP-class
protein binding [PMID:8702872]"
/experiment="EXISTENCE:direct assay:GO:0042790 nucleolar
large rRNA transcription by RNA polymerase I
[PMID:7958901]"
/experiment="EXISTENCE:direct assay:GO:0070860 RNA
polymerase I core factor complex
[PMID:8887672|PMID:8702872|PMID:7958901]"
/experiment="EXISTENCE:mutant phenotype:GO:0042790
nucleolar large rRNA transcription by RNA polymerase I
[PMID:7958901]"
/experiment="EXISTENCE:physical interaction:GO:0017025
TBP-class protein binding [PMID:8702872]"
/note="Component of the core factor (CF) rDNA
transcription factor complex; CF is required for
transcription of 35S rRNA genes by RNA polymerase I and is
composed of Rrn6p, Rrn7p, and Rrn11p"
/codon_start=1
/product="Rrn7p"
/protein_id="NP_012509.1"
/db_xref="GeneID:853428"
/db_xref="SGD:S000003562"
/translation="MSTFIRGPICGTDNCPSRLWRIIDGRRTCQYGHVMEGDVEFNDD
EDDLNGLGAGVITRRLNLTTNATGSFQSSQLTNSQLLQQQQRQSHKKFKKLIGHEAKL
LFLKSFQFILKRQIRWLITEMRFPKEFEHVAKIIWLKILKTINDQPQEELKLQLHMTS
TISILYLASTHLSLPVYTCDYIKWICTAKMPYFQASEILPKSWRIQLPNYYVSILEGS
ISPFNGQLYNKIALTCGMIHFKEFFNSEISCQGLLLKLVMQCALPPEFYFYTKQVIEF
EETDIRNLTLWERTDERHTGRVSNHAELRVLSYFMLTINWMLSFDRDRQYPLKWILSL
TESLTQRTTTSESIGRNIVKVVYPDKPTSSDYFQWSEEETLEFLKWMEKQFLPTQTKS
LHNENGSMEMTIDQKIARRKLYKIFPLDREANHDGEFNDSTHQLTFIEDLQERYAKQT
PFFESNKIRDSLNYQEANPPARKEAIGRLLTHIASQLLVDFAISKEQLKDCISRIKNA
CLHRMN"
gene complement(<395931..>396592)
/gene="APS3"
/locus_tag="YJL024C"
/gene_synonym="YKS7"
/db_xref="GeneID:853429"
mRNA complement(join(<395931..396493,396571..>396592))
/gene="APS3"
/locus_tag="YJL024C"
/gene_synonym="YKS7"
/product="Aps3p"
/transcript_id="NM_001181458.3"
/db_xref="GeneID:853429"
CDS complement(join(395931..396493,396571..396592))
/gene="APS3"
/locus_tag="YJL024C"
/gene_synonym="YKS7"
/experiment="EXISTENCE:mutant phenotype:GO:0006623 protein
targeting to vacuole [PMID:17895371]"
/experiment="EXISTENCE:mutant phenotype:GO:0006896 Golgi
to vacuole transport [PMID:9335339]"
/experiment="EXISTENCE:mutant phenotype:GO:0030123 AP-3
adaptor complex [PMID:9250663]"
/note="Small subunit of the clathrin-associated adaptor
complex AP-3; involved in vacuolar protein sorting;
related to the sigma subunit of the mammalian clathrin
AP-3 complex; suppressor of loss of casein kinase 1
function; protein abundance increases in response to DNA
replication stress"
/codon_start=1
/product="Aps3p"
/protein_id="NP_012510.3"
/db_xref="GeneID:853429"
/db_xref="SGD:S000003561"
/translation="MIHAVLIFNKKCQPRLVKFYTPVDLPKQKLLLEQVYELISQRNS
DFQSSFLVTPPSLLLSNENNNDEVNNEDIQIIYKNYATLYFTFIVDDQESELAILDLI
QTFVESLDRCFTEVNELDLIFNWQTLESVLEEIVQGGMVIETNVNRIVASVDELNKAA
ESTDSKIGRLTSTGFGSALQAFAQGGFAQWATGQ"
gene complement(396726..396796)
/locus_tag="YNCJ0017C"
/db_xref="GeneID:853430"
tRNA complement(396726..396796)
/locus_tag="YNCJ0017C"
/product="tRNA-Gly"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0030371 translation
repressor activity [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043555 regulation
of translation in response to stress [PMID:27609601]"
/note="Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:853430"
/db_xref="SGD:S000006583"
gene complement(<397355..>398398)
/gene="PET130"
/locus_tag="YJL023C"
/db_xref="GeneID:853432"
mRNA complement(<397355..>398398)
/gene="PET130"
/locus_tag="YJL023C"
/product="Pet130p"
/transcript_id="NM_001181457.1"
/db_xref="GeneID:853432"
CDS complement(397355..398398)
/gene="PET130"
/locus_tag="YJL023C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0099617 matrix side
of mitochondrial inner membrane [PMID:30759361]"
/experiment="EXISTENCE:mutant phenotype:GO:0097745
mitochondrial tRNA 5'-end processing [PMID:30759361]"
/note="hypothetical protein; required for efficient 5'
processing of mitochondrial tRNAs, for respiratory growth
and mitochondrial genome maintenance; localizes to the
matrix side of the inner mitochondrial membrane"
/codon_start=1
/product="Pet130p"
/protein_id="NP_012511.1"
/db_xref="GeneID:853432"
/db_xref="SGD:S000003560"
/translation="MKIFSTLLSQKPKGKLVIRPSTTIHSSDPFSKFIVTKNTEPLSL
GDLRKSDSGNSAVCLNAENTILSTLTDLQKEEERNWDPVKFVAGKLRGVISPIQAYVT
IGKKFSPNSLVYTSRFFQLHYFPEDHFMSCFRKSKPAITVKSNKKFYLNGKVFNKDKE
YFNETRISKANEVELSKIQTAMTRLTNRHRNSIPSEFAYLRRDLKLKVKTTFIKEWCK
LNGDKAIREYVNLNRSPNINPASMKGKPKKSFLDNLGRSTVGTAKDGYYLYIVSIFPD
KDMLGEFNDEVNRSVQKVANLDWDGFLTPKKGTKGKNWVESFNDSINVQTINKILEIN
KFPFELRREQTEG"
gene complement(<398937..>402410)
/gene="BBC1"
/locus_tag="YJL020C"
/gene_synonym="MTI1; YJL021C"
/db_xref="GeneID:853433"
mRNA complement(<398937..>402410)
/gene="BBC1"
/locus_tag="YJL020C"
/gene_synonym="MTI1; YJL021C"
/product="Bbc1p"
/transcript_id="NM_001181454.1"
/db_xref="GeneID:853433"
CDS complement(398937..402410)
/gene="BBC1"
/locus_tag="YJL020C"
/gene_synonym="MTI1; YJL021C"
/experiment="EXISTENCE:direct assay:GO:0030479 actin
cortical patch [PMID:16824951|PMID:11743162]"
/experiment="EXISTENCE:direct assay:GO:0032032 myosin I
tail binding [PMID:11901111]"
/experiment="EXISTENCE:direct assay:GO:0034316 negative
regulation of Arp2/3 complex-mediated actin nucleation
[PMID:12814545]"
/experiment="EXISTENCE:genetic interaction:GO:0030036
actin cytoskeleton organization [PMID:11901111]"
/experiment="EXISTENCE:physical interaction:GO:0032032
myosin I tail binding [PMID:11901111]"
/note="Protein possibly involved in assembly of actin
patches; interacts with an actin assembly factor Las17p
and with the SH3 domains of Type I myosins Myo3p and
Myo5p; localized predominantly to cortical actin patches"
/codon_start=1
/product="Bbc1p"
/protein_id="NP_012514.2"
/db_xref="GeneID:853433"
/db_xref="SGD:S000003557"
/translation="MSEPEVPFKVVAQFPYKSDYEDDLNFEKDQEIIVTSVEDAEWYF
GEYQDSNGDVIEGIFPKSFVAVQGSEVGKEAESSPNTGSTEQRTIQPEVEQKDLPEPI
SPETKKETLSGPVPVPAATVPVPAATVPVPAATAVSAQVQHDSSSGNGERKVPMDSPK
LKARLSMFNQDITEQVPLPKSTHLDLENIPVKKTIVADAPKYYVPPGIPTNDTSNLER
KKSLKENEKKIVPEPINRAQVESGRIETENDQLKKDLPQMSLKERIALLQEQQRLQAA
REEELLRKKAKLEQEHERSAVNKNEPYTETEEAEENEKTEPKPEFTPETEHNEEPQME
LLAHKEITKTSREADEGTNDIEKEQFLDEYTKENQKVEESQADEARGENVAEESEIGY
GHEDREGDNDEEKEEEDSEENRRAALRERMAKLSGASRFGAPVGFNPFGMASGVGNKP
SEEPKKKQHKEKEEEEPEQLQELPRAIPVMPFVDPSSNPFFRKSNLSEKNQPTETKTL
DPHATTEHEQKQEHGTHAYHNLAAVDNAHPEYSDHDSDEDTDDHEFEDANDGLRKHSM
VEQAFQIGNNESENVNSGEKIYPQEPPISHRTAEVSHDIENSSQNTTGNVLPVSSPQT
RVARNGSINSLTKSISGENRRKSINEYHDTVSTNSSALTETAQDISMAAPAAPVLSKV
SHPEDKVPPHPVPSAPSAPPVPSAPSVPSAPPVPPAPPALSAPSVPPVPPVPPVSSAP
PALSAPSIPPVPPTPPAPPAPPAPLALPKHNEVEEHVKSSAPLPPVSEEYHPMPNTAP
PLPRAPPVPPATFEFDSEPTATHSHTAPSPPPHQNVTASTPSMMSTQQRVPTSVLSGA
EKESRTLPPHVPSLTNRPVDSFHESDTTPKVASIRRSTTHDVGEISNNVKIEFNAQER
WWINKSAPPAISNLKLNFLMEIDDHFISKRLHQKWVVRDFYFLFENYSQLRFSLTFNS
TSPEKTVTTLQERFPSPVETQSARILDEYAQRFNAKVVEKSHSLINSHIGAKNFVSQI
VSEFKDEVIQPIGARTFGATILSYKPEEGIEQLMKSLQKIKPGDILVIRKAKFEAHKK
IGKNEIINVGMDSAAPYSSVVTDYDFTKNKFRVIENHEGKIIQNSYKLSHMKSGKLKV
FRIVARGYVGW"
gene <402897..>404945
/gene="MPS3"
/locus_tag="YJL019W"
/gene_synonym="NEP98; YJL018W"
/db_xref="GeneID:853434"
mRNA <402897..>404945
/gene="MPS3"
/locus_tag="YJL019W"
/gene_synonym="NEP98; YJL018W"
/product="Mps3p"
/transcript_id="NM_001181453.3"
/db_xref="GeneID:853434"
CDS 402897..404945
/gene="MPS3"
/locus_tag="YJL019W"
/gene_synonym="NEP98; YJL018W"
/experiment="EXISTENCE:direct assay:GO:0000781 chromosome,
telomeric region [PMID:17495028]"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:17495028]"
/experiment="EXISTENCE:direct assay:GO:0005816 spindle
pole body [PMID:16923827]"
/experiment="EXISTENCE:direct assay:GO:0005825 half bridge
of spindle pole body [PMID:12486115]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:12493774|PMID:12486115]"
/experiment="EXISTENCE:direct assay:GO:0034399 nuclear
periphery [PMID:26928762|PMID:18039933]"
/experiment="EXISTENCE:genetic interaction:GO:0007129
homologous chromosome pairing at meiosis [PMID:17495028]"
/experiment="EXISTENCE:genetic interaction:GO:0045141
meiotic telomere clustering [PMID:17495028]"
/experiment="EXISTENCE:mutant phenotype:GO:0000741
karyogamy [PMID:16923827]"
/experiment="EXISTENCE:mutant phenotype:GO:0000743 nuclear
migration involved in conjugation with cellular fusion
[PMID:12493774]"
/experiment="EXISTENCE:mutant phenotype:GO:0007064 mitotic
sister chromatid cohesion [PMID:15355977]"
/experiment="EXISTENCE:mutant phenotype:GO:0007129
homologous chromosome pairing at meiosis [PMID:17495028]"
/experiment="EXISTENCE:mutant phenotype:GO:0030474 spindle
pole body duplication [PMID:16923827|PMID:12486115]"
/experiment="EXISTENCE:mutant phenotype:GO:0031509
subtelomeric heterochromatin formation [PMID:22593213]"
/experiment="EXISTENCE:mutant phenotype:GO:0034087
establishment of mitotic sister chromatid cohesion
[PMID:22593213]"
/experiment="EXISTENCE:mutant phenotype:GO:0034398
telomere tethering at nuclear periphery
[PMID:22593213|PMID:18039933]"
/experiment="EXISTENCE:mutant phenotype:GO:0045141 meiotic
telomere clustering [PMID:17495028]"
/note="Nuclear envelope protein; required for SPB
insertion, SPB duplication, Kar5p localization near the
SPB and nuclear fusion; interacts with Mps2p to tether
half-bridge to core SPB; N-terminal acetylation by Eco1p
regulates its role in nuclear organization; localizes to
the SPB half bridge and telomeres during meiosis; required
with Ndj1p and Csm4p for meiotic bouquet formation and
telomere-led rapid prophase movement; member of the SUN
protein family (Sad1-UNC-84 homology)"
/codon_start=1
/product="Mps3p"
/protein_id="NP_012515.4"
/db_xref="GeneID:853434"
/db_xref="SGD:S000003556"
/translation="MNNSNEHRREEAGAANEQMPYNKAVKSAYADVLKDKMNREQEIS
LRAIKKGIYTDGGETDNYDMDKENDSAYEMFKKNLDFPLDQHNDDDDDDPYIEDNGQE
TDGYSDEDYTDEADKSFIEDSDSDSYDLESNSDFEENLESSGEAKKLKWRTYIFYGGL
FFVFYFFGSFLMTTVKNNDLESHSSGATSSPGKSFSNLQKQVNHLYSELSKRDEKHSS
ELDKTVKIIVSQFEKNIKRLLPSNLVNFENDINSLTKQVETISTSMSELQRRNHKFTV
ENVTQWQDQLVKQLDTHLPQEIPVVINNSSSLLIIPELHNYLSALISDVIESPGIGTA
GSAESRWEYDLNRYVKEILSNELQYIDKDYFIQEMNRRLQSNKQEIWEEITNRLETQQ
QQQQQQVQQDYSNVPQQYSSILMKRLIHQIYNSNQHQWEDDLDFATYVQGTKLLNHLT
SPTWRQGSGVQPIELLTDSKQSSSTYWQCENEPGCSWAIRFKTPLYLTKISYMHGRFT
NNLHIMNSAPRLISLYVKLSQTKEIKALQTLANQYGFGQHHKRDRNYIKIAKFEYRLT
DSRIRQQMYLPPWFIQLKPLVRSIVFQVDENYGNKKFISLRKFIINGVTPQDLQIIEN
NEFPVLLGDTPEYGVTQNTDEGKRKVLLSKPPYASSSTSTKFHPASNVPSFGQDELDQ
"
gene <405588..>407273
/gene="TPH3"
/locus_tag="YJL016W"
/gene_synonym="YJL017W"
/db_xref="GeneID:853436"
mRNA <405588..>407273
/gene="TPH3"
/locus_tag="YJL016W"
/gene_synonym="YJL017W"
/product="Tph3p"
/transcript_id="NM_001181450.1"
/db_xref="GeneID:853436"
CDS 405588..407273
/gene="TPH3"
/locus_tag="YJL016W"
/gene_synonym="YJL017W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="hypothetical protein; GFP-fusion protein localizes
to the cytoplasm; contains two adjacent PH-like domains;
conserved in closely related Saccharomyces species"
/codon_start=1
/product="Tph3p"
/protein_id="NP_012518.2"
/db_xref="GeneID:853436"
/db_xref="SGD:S000003553"
/translation="MGILDSLKKPVNIKSSLPKFSRSATSINLSSHPVSSRSFLELPP
ELFSITKPIFKLLQAHANKIYFQSSDAEAVWNVKDSSGHVFEAESISLLGSHIIITNS
SVQVADVAIIDSPSNINQCEISSVGEFLQFNNGQLSITCNDFGLLEKFKRLCMISIFE
FISIYKALTGTVISSYGLRMSDMHIILNSPFNFKDWCEVYLDGQGWVKVWCHIDKVSK
TNNSKSSSDNDAKGKYQIRFYRDDKSTSSKNCVFFIPDNEYVQDIFFYNINAAEPSKN
MNDFFQGLQMIKLVGNVRFCSDTDFNDVVDSGSIYSSANNGSGDSSSTALNNESPNTT
PKSRTFFSPKGHRRNSSHVSSLTSRSTKKPITNFTTRTNGLLIRPLPHHGVHHLEAMI
RFIIPLMDCARLYGRPVQFKTERTDINSLMFGLPKLPSVDYFAEEEIAHLMTQEFNPL
KEKDTDDTMALTMSRFSSYLQERMTKVSKRNTELNFRTFSDVMGMYNTTRDHSKLNCM
SDKDNSVKEFSLSDKSNVSSETTNMMNQLQVNAHEYKSSMCERPIVASTSPIA"
gene <407558..>409162
/gene="CCT3"
/locus_tag="YJL014W"
/gene_synonym="BIN2; TCP3"
/db_xref="GeneID:853438"
mRNA <407558..>409162
/gene="CCT3"
/locus_tag="YJL014W"
/gene_synonym="BIN2; TCP3"
/product="chaperonin-containing T-complex subunit CCT3"
/transcript_id="NM_001181448.1"
/db_xref="GeneID:853438"
CDS 407558..409162
/gene="CCT3"
/locus_tag="YJL014W"
/gene_synonym="BIN2; TCP3"
/experiment="EXISTENCE:direct assay:GO:0005832
chaperonin-containing T-complex [PMID:16762366]"
/experiment="EXISTENCE:direct assay:GO:0006457 protein
folding [PMID:16762366]"
/experiment="EXISTENCE:direct assay:GO:0051082 unfolded
protein binding [PMID:16762366]"
/experiment="EXISTENCE:physical interaction:GO:0005832
chaperonin-containing T-complex [PMID:15704212]"
/note="Subunit of the cytosolic chaperonin Cct ring
complex; related to Tcp1p, required for the assembly of
actin and tubulins in vivo; capable of binding Q/N rich
proteins and mediating their folding"
/codon_start=1
/product="chaperonin-containing T-complex subunit CCT3"
/protein_id="NP_012520.1"
/db_xref="GeneID:853438"
/db_xref="SGD:S000003551"
/translation="MQAPVVFMNASQERTTGRQAQISNITAAKAVADVIRTCLGPKAM
LKMLLDPMGGLVLTNDGHAILREIDVAHPAAKSMLELSRTQDEEVGDGTTTVIILAGE
ILAQCAPYLIEKNIHPVIIIQALKKALTDALEVIKQVSKPVDVENDAAMKKLIQASIG
TKYVIHWSEKMCELALDAVKTVRKDLGQTVEGEPNFEIDIKRYVRVEKIPGGDVLDSR
VLKGVLLNKDVVHPKMSRHIENPRVVLLDCPLEYKKGESQTNIEIEKEEDWNRILQIE
EEQVQLMCEQILAVRPTLVITEKGVSDLAQHYLLKGGCSVLRRVKKSDNNRIARVTGA
TIVNRVEDLKESDVGTNCGLFKVEMIGDEYFSFLDNCKEPKACTIMLRGGSKDILNEI
DRNLQDAMAVARNVMLSPSLSPGGGATEMAVSVKLAEKAKQLEGIQQWPYQAVADAME
CIPRTLIQNAGGDPIRLLSQLRAKHAQGNFTTGIDGDKGKIVDMVSYGIWEPEVIKQQ
SVKTAIESACLLLRVDDIVSGVRKQE"
gene complement(<409493..>411040)
/gene="MAD3"
/locus_tag="YJL013C"
/db_xref="GeneID:853439"
mRNA complement(<409493..>411040)
/gene="MAD3"
/locus_tag="YJL013C"
/product="Mad3p"
/transcript_id="NM_001181447.3"
/db_xref="GeneID:853439"
CDS complement(409493..411040)
/gene="MAD3"
/locus_tag="YJL013C"
/experiment="EXISTENCE:direct assay:GO:0033597 mitotic
checkpoint complex [PMID:11726501]"
/experiment="EXISTENCE:direct assay:GO:1902499 positive
regulation of protein autoubiquitination [PMID:22940250]"
/experiment="EXISTENCE:genetic interaction:GO:0044774
mitotic DNA integrity checkpoint signaling
[PMID:16651657]"
/experiment="EXISTENCE:mutant phenotype:GO:0007094 mitotic
spindle assembly checkpoint signaling [PMID:10704439]"
/experiment="EXISTENCE:mutant phenotype:GO:0032837
distributive segregation [PMID:15951820]"
/experiment="EXISTENCE:mutant phenotype:GO:0033597 mitotic
checkpoint complex [PMID:10704439]"
/experiment="EXISTENCE:physical interaction:GO:0033597
mitotic checkpoint complex [PMID:10704439|PMID:11726501]"
/note="Subunit of the spindle-assembly checkpoint complex;
involved in delaying anaphase onset in cells with defects
in mitotic spindle assembly; pseudosubstrate inhibitor of
APC(Cdc20), the anaphase promoting complex involved in
Pds1p (securin) turnover; MAD3 has a highly diverged
paralog, BUB1, that arose from the whole genome
duplication; N-terminal TPR domain-containing region is
homologous with the N-terminal region of Bub1p"
/codon_start=1
/product="Mad3p"
/protein_id="NP_012521.3"
/db_xref="GeneID:853439"
/db_xref="SGD:S000003550"
/translation="MKAYAKKRISYMPSSPSQNVINFEEIETQKENILPLKEGRSAAA
LSKAIHQPLVEINQVKSSFEQRLIDELPALSDPITLYLEYIKWLNNAYPQGGNSKQSG
MLTLLERCLSHLKDLERYRNDVRFLKIWFWYIELFTRNSFMESRDIFMYMLRNGIGSE
LASFYEEFTNLLIQKEKFQYAVKILQLGIKNKARPNKVLEDRLNHLLRELGENNIQLG
NEISMDSLESTVLGKTRSEFVNRLELANQNGTSSDVNLTKNNVFVDGEESDVELFETP
NRGVYRDGWENFDLKAERNKENNLRISLLEANTNLGELKQHEMLSQKKRPYDEKLPIF
RDSIGRSDPVYQMINTKDQKPEKIDCNFKLIYCEDEESKGGRLEFSLEEVLAISRNVY
KRVRTNRKHPREANLGQEESANQKEAEAQSKRPKISRKALVSKSLTPSNQGRMFSGEE
YINCPMTPKGRSTETSDIISAVKPRQLTPILEMRESNSFSQSKNSEIISDDDKSSSSF
ISYPPQR"
gene complement(<411234..>413399)
/gene="VTC4"
/locus_tag="YJL012C"
/gene_synonym="PHM3; YJL012C-A"
/db_xref="GeneID:853441"
mRNA complement(<411234..>413399)
/gene="VTC4"
/locus_tag="YJL012C"
/gene_synonym="PHM3; YJL012C-A"
/product="Vtc4p"
/transcript_id="NM_001181446.1"
/db_xref="GeneID:853441"
CDS complement(411234..413399)
/gene="VTC4"
/locus_tag="YJL012C"
/gene_synonym="PHM3; YJL012C-A"
/EC_number="2.7.4.1"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0000822 inositol
hexakisphosphate binding [PMID:27080106]"
/experiment="EXISTENCE:direct assay:GO:0005516 calmodulin
binding [PMID:17079729]"
/experiment="EXISTENCE:direct assay:GO:0005774 vacuolar
membrane [PMID:12584253]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:17079729]"
/experiment="EXISTENCE:direct assay:GO:0007034 vacuolar
transport [PMID:17079729]"
/experiment="EXISTENCE:direct assay:GO:0008976
polyphosphate kinase activity [PMID:19390046]"
/experiment="EXISTENCE:direct assay:GO:0016237
microautophagy [PMID:17079729]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0006797
polyphosphate metabolic process
[PMID:19390046|PMID:11102525]"
/experiment="EXISTENCE:mutant phenotype:GO:0006799
polyphosphate biosynthetic process
[PMID:27252384|PMID:27080106]"
/experiment="EXISTENCE:mutant phenotype:GO:0007034
vacuolar transport [PMID:17079729]"
/experiment="EXISTENCE:mutant phenotype:GO:0016237
microautophagy [PMID:17079729]"
/experiment="EXISTENCE:physical interaction:GO:0033254
vacuolar transporter chaperone complex [PMID:11823419]"
/note="Vacuolar membrane polyphosphate polymerase; subunit
of vacuolar transporter chaperone (VTC) complex involved
in synthesis and transfer of polyP to vacuole; regulates
membrane trafficking; role in non-autophagic vacuolar
fusion; targeted to vacuole via AP-3 pathway; protein
abundance increases in response to DNA replication stress"
/codon_start=1
/product="Vtc4p"
/protein_id="NP_012522.2"
/db_xref="GeneID:853441"
/db_xref="SGD:S000003549"
/translation="MKFGEHLSKSLIRQYSYYYISYDDLKTELEDNLSKNNGQWTQEL
ETDFLESLEIELDKVYTFCKVKHSEVFRRVKEVQEQVQHTVRLLDSNNPPTQLDFEIL
EEELSDIIADVHDLAKFSRLNYTGFQKIIKKHDKKTGFILKPVFQVRLDSKPFFKENY
DELVVKISQLYDIARTSGRPIKGDSSAGGKQQNFVRQTTKYWVHPDNITELKLIILKH
LPVLVFNTNKEFEREDSAITSIYFDNENLDLYYGRLRKDEGAEAHRLRWYGGMSTDTI
FVERKTHREDWTGEKSVKARFALKERHVNDFLKGKYTVDQVFAKMRKEGKKPMNEIEN
LEALASEIQYVMLKKKLRPVVRSFYNRTAFQLPGDARVRISLDTELTMVREDNFDGVD
RTHKNWRRTDIGVDWPFKQLDDKDICRFPYAVLEVKLQTQLGQEPPEWVRELVGSHLV
EPVPKFSKFIHGVATLLNDKVDSIPFWLPQMDVDIRKPPLPTNIEITRPGRSDNEDND
FDEDDEDDAALVAAMTNAPGNSLDIEESVGYGATSAPTSNTNHVVESANAAYYQRKIR
NAENPISKKYYEIVAFFDHYFNGDQISKIPKGTTFDTQIRAPPGKTICVPVRVEPKVY
FATERTYLSWLSISILLGGVSTTLLTYGSPTAMIGSIGFFITSLAVLIRTVMVYAKRV
VNIRLKRAVDYEDKIGPGMVSVFLILSILFSFFCNLVAK"
gene complement(<414285..>414770)
/gene="RPC17"
/locus_tag="YJL011C"
/db_xref="GeneID:853442"
mRNA complement(<414285..>414770)
/gene="RPC17"
/locus_tag="YJL011C"
/product="DNA-directed RNA polymerase III subunit RPC17"
/transcript_id="NM_001181445.2"
/db_xref="GeneID:853442"
CDS complement(414285..414770)
/gene="RPC17"
/locus_tag="YJL011C"
/experiment="EXISTENCE:direct assay:GO:0003899
DNA-directed RNA polymerase activity
[PMID:3905793|PMID:10611227]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005666 RNA
polymerase III complex [PMID:10611227|PMID:3905793]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0042797 tRNA
transcription by RNA polymerase III [PMID:3905793]"
/experiment="EXISTENCE:mutant phenotype:GO:0042797 tRNA
transcription by RNA polymerase III [PMID:10611227]"
/experiment="EXISTENCE:physical interaction:GO:0006384
transcription initiation at RNA polymerase III promoter
[PMID:10611227]"
/note="RNA polymerase III subunit C17; physically
interacts with C31, C11, and TFIIIB70; may be involved in
the recruitment of pol III by the preinitiation complex;
protein abundance increases in response to DNA replication
stress; relocalizes to the cytosol in response to hypoxia"
/codon_start=1
/product="DNA-directed RNA polymerase III subunit RPC17"
/protein_id="NP_012523.2"
/db_xref="GeneID:853442"
/db_xref="SGD:S000003548"
/translation="MKVLEERNAFLSDYEVLKFLTDLEKKHLWDQKSLAALKKSRSKG
KQNRPYNHPELQGITRNVVNYLSINKNFINQEDEGEERESSGAKDAEKSGISKMSDES
FAELMTKLNSFKLFKAEKLQIVNQLPANMVHLYSIVEECDARFDEKTIEEMLEIISGY
A"
gene 414966..415038
/locus_tag="YNCJ0018W"
/db_xref="GeneID:853443"
tRNA 414966..415038
/locus_tag="YNCJ0018W"
/product="tRNA-Lys"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:8915539]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:8915539]"
/experiment="EXISTENCE:mutant phenotype:GO:0030533 triplet
codon-amino acid adaptor activity [PMID:17560369]"
/experiment="EXISTENCE:mutant phenotype:GO:0032543
mitochondrial translation [PMID:17560369]"
/note="Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE
analysis; a small portion is imported into mitochondria
via interaction with mt lysyl-tRNA synthetase Msk1p and is
necessary to decode AAG codons at high temperature, when
base modification of mt-encoded tRNA-Lys is reduced"
/db_xref="GeneID:853443"
/db_xref="SGD:S000006625"
gene complement(415931..416036)
/locus_tag="YNCJ0019C"
/db_xref="GeneID:853444"
tRNA complement(join(415931..415966,416001..416036))
/locus_tag="YNCJ0019C"
/product="tRNA-Trp"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Tryptophan tRNA (tRNA-Trp), predicted by
tRNAscan-SE analysis"
/db_xref="GeneID:853444"
/db_xref="SGD:S000006773"
repeat_region 416266..416441
/note="Ty4 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007047"
repeat_region 416506..416650
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007029"
repeat_region 416899..417026
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007030"
rep_origin 417195..417440
/note="ARS1014; Autonomously Replicating Sequence"
/db_xref="SGD:S000028451"
gene complement(<417562..>419562)
/gene="NOP9"
/locus_tag="YJL010C"
/db_xref="GeneID:853445"
mRNA complement(<417562..>419562)
/gene="NOP9"
/locus_tag="YJL010C"
/product="RNA-binding RNA processing protein NOP9"
/transcript_id="NM_001181444.3"
/db_xref="GeneID:853445"
CDS complement(417562..419562)
/gene="NOP9"
/locus_tag="YJL010C"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:17956976]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14690591|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:17956976|PMID:14690591|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:17956976]"
/experiment="EXISTENCE:direct assay:GO:0030688
preribosome, small subunit precursor [PMID:17956976]"
/experiment="EXISTENCE:mutant phenotype:GO:0000056
ribosomal small subunit export from nucleus
[PMID:17956976]"
/experiment="EXISTENCE:mutant phenotype:GO:0000447
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from
5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:17956976]"
/experiment="EXISTENCE:mutant phenotype:GO:0000472
endonucleolytic cleavage to generate mature 5'-end of
SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:17956976]"
/experiment="EXISTENCE:mutant phenotype:GO:0000480
endonucleolytic cleavage in 5'-ETS of tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:17956976]"
/note="Essential subunit of U3-containing 90S preribosome;
involved in production of 18S rRNA and assembly of small
ribosomal subunit; also part of pre-40S ribosome and
required for its export into cytoplasm; binds RNA and
contains pumilio domain"
/codon_start=1
/product="RNA-binding RNA processing protein NOP9"
/protein_id="NP_012524.3"
/db_xref="GeneID:853445"
/db_xref="SGD:S000003547"
/translation="MGKTKTRGRRHQDKQRKDEFEPSSNSAKEHIQQEESTYNDEAEI
KETQPQMFFGVLDREELEYFKQAESTLQLDAFEAPEEKFQFVTSIIEEAKGKELKLVT
SQITSKLMERVILECDETQLKDIFQSFNGVFFGLSCHKYASHVLETLFVRSAALVERE
LLTPSFDNNEKEGPYVTMENMFLFMLNELKPHLKTMMNHQYASHVLRLLILILSSKTL
PNSTKANSTLRSKKSKIARKMIDIKDNDDFNKVYQTPESFKSELRDIITTLYKGFTNG
AESRSDISQSTITKFREYSVDKVASPVIQLIIQVEGIFDRDRSFWRLVFNTADEKDPK
EESFLEYLLSDPVGSHFLENVIGSARLKYVERLYRLYMKDRIVKLAKRDTTGAFVVRA
LLEHLKEKDVKQILDAVVPELSMLLNSNMDFGTAIINASNKQGGYLRDDVIAQLIQKY
YPEKSDAKNILESCLLLSASTLGNTRDDWPTAEERRRSVFLEQLIDYDDKFLNITIDS
MLALPEERLIQMCYHGVFSHVVEHVLQTTRVDIIKRKMLLNILSKESVNLACNVYGSH
IMDKLWEFTAKLTLYKERIARALVLETEKVKNSIYGRQVWKNWKLELYVRKMWDWKKL
IKEQEFEIFPNSKPLQPKPEKHSRERNNSKEGSAFKKQKHYR"
gene complement(<419957..>421663)
/gene="CCT8"
/locus_tag="YJL008C"
/db_xref="GeneID:853447"
mRNA complement(<419957..>421663)
/gene="CCT8"
/locus_tag="YJL008C"
/product="chaperonin-containing T-complex subunit CCT8"
/transcript_id="NM_001181442.2"
/db_xref="GeneID:853447"
CDS complement(419957..421663)
/gene="CCT8"
/locus_tag="YJL008C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005832
chaperonin-containing T-complex [PMID:16762366]"
/experiment="EXISTENCE:direct assay:GO:0006457 protein
folding [PMID:16762366]"
/experiment="EXISTENCE:direct assay:GO:0051082 unfolded
protein binding [PMID:16762366]"
/experiment="EXISTENCE:physical interaction:GO:0005832
chaperonin-containing T-complex [PMID:15704212]"
/note="Subunit of the cytosolic chaperonin Cct ring
complex; related to Tcp1p, required for the assembly of
actin and tubulins in vivo"
/codon_start=1
/product="chaperonin-containing T-complex subunit CCT8"
/protein_id="NP_012526.1"
/db_xref="GeneID:853447"
/db_xref="SGD:S000003545"
/translation="MSLRLPQNPNAGLFKQGYNSYSNADGQIIKSIAAIRELHQMCLT
SMGPCGRNKIIVNHLGKIIITNDAATMLRELDIVHPAVKVLVMATEQQKIDMGDGTNL
VMILAGELLNVSEKLISMGLSAVEIIQGYNMARKFTLKELDEMVVGEITDKNDKNELL
KMIKPVISSKKYGSEDILSELVSEAVSHVLPVAQQAGEIPYFNVDSIRVVKIMGGSLS
NSTVIKGMVFNREPEGHVKSLSEDKKHKVAVFTCPLDIANTETKGTVLLHNAQEMLDF
SKGEEKQIDAMMKEIADMGVECIVAGAGVGELALHYLNRYGILVLKVPSKFELRRLCR
VCGATPLPRLGAPTPEELGLVETVKTMEIGGDRVTVFKQEQGEISRTSTIILRGATQN
NLDDIERAIDDGVAAVKGLMKPSGGKLLPGAGATEIELISRITKYGERTPGLLQLAIK
QFAVAFEVVPRTLAETAGLDVNEVLPNLYAAHNVTEPGAVKTDHLYKGVDIDGESDEG
VKDIREENIYDMLATKKFAINVATEAATTVLSIDQIIMAKKAGGPRAPQGPRPGNWDQ
ED"
repeat_region 421946..422295
/note="Ty4 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007038"
repeat_region 422601..422887
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007031"
gene complement(<422698..>423012)
/locus_tag="YJL007C"
/db_xref="GeneID:853449"
mRNA complement(<422698..>423012)
/locus_tag="YJL007C"
/product="uncharacterized protein"
/transcript_id="NM_001348847.1"
/db_xref="GeneID:853449"
CDS complement(422698..423012)
/locus_tag="YJL007C"
/note="hypothetical protein; conserved among S. cerevisiae
strains"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335787.1"
/db_xref="GeneID:853449"
/db_xref="SGD:S000003544"
/translation="MCSRGGSNSRPSDYETDALPTELLKHTKDVGEEKQTLHQIFADS
MVIKGYSTGYTGHTRSSPGDLVIHKRELIFSHNIVIIVSPIYMISFIILLHYQSWHFS
IY"
gene 422937..423009
/gene="EMT3"
/locus_tag="YNCJ0020W"
/db_xref="GeneID:853448"
tRNA 422937..423009
/gene="EMT3"
/locus_tag="YNCJ0020W"
/product="tRNA-Met"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Methionine tRNA (tRNA-Met), functions in
translational elongation; not involved in translational
initiation; predicted by tRNAscan-SE analysis"
/db_xref="GeneID:853448"
/db_xref="SGD:S000006662"
gene complement(<423138..>424109)
/gene="CTK2"
/locus_tag="YJL006C"
/db_xref="GeneID:853450"
mRNA complement(<423138..>424109)
/gene="CTK2"
/locus_tag="YJL006C"
/product="Ctk2p"
/transcript_id="NM_001181440.1"
/db_xref="GeneID:853450"
CDS complement(423138..424109)
/gene="CTK2"
/locus_tag="YJL006C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005654 nucleoplasm
[PMID:15520468]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:15520468]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0016538
cyclin-dependent protein serine/threonine kinase regulator
activity [PMID:11118453]"
/experiment="EXISTENCE:direct assay:GO:0032786 positive
regulation of DNA-templated transcription, elongation
[PMID:9110987]"
/experiment="EXISTENCE:direct assay:GO:0070692 CTDK-1
complex [PMID:7565723]"
/experiment="EXISTENCE:mutant phenotype:GO:0045943
positive regulation of transcription by RNA polymerase I
[PMID:15520468]"
/experiment="EXISTENCE:physical interaction:GO:0045903
positive regulation of translational fidelity
[PMID:17545469]"
/note="Beta subunit of C-terminal domain kinase I
(CTDK-I); which phosphorylates both RNA pol II subunit
Rpo21p to affect transcription and pre-mRNA 3' end
processing, and ribosomal protein Rps2p to increase
translational fidelity; relocalizes to the cytosol in
response to hypoxia"
/codon_start=1
/product="Ctk2p"
/protein_id="NP_012528.1"
/db_xref="GeneID:853450"
/db_xref="SGD:S000003543"
/translation="MPSTFESQLFFSRPFLSKRQIQRAQKNTISDYRNYNQKKLAVFK
FLSDLCVQLKFPRKTLETAVYFYQRYHLFNRFETEVCYTVATSCLTLGCKEVETIKKT
NDICTLSLRLRNVVKINTDILENFKKRVFQIELRILESCSFDYRVNNYVHIDEYVIKI
GRELSFDYKLCNLAWVIAYDALKLETILVIPQHSIALAILKIAYELLDNKNWSSKRYS
LFETDEKSVNEAYFDIVNFYINSFDMCDLQRHLPADLLPIGVERFMELKKNAGPESGL
PQIPDHLLNADPYITITRDNNVQERRYVLSLELINGESSINSSTRHA"
gene complement(424432..424515)
/gene="SUP51"
/locus_tag="YNCJ0021C"
/db_xref="GeneID:853451"
tRNA complement(424432..424515)
/gene="SUP51"
/locus_tag="YNCJ0021C"
/product="tRNA-Leu"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0030371 translation
repressor activity [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043555 regulation
of translation in response to stress [PMID:27609601]"
/note="Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE
analysis; can mutate to suppress amber nonsense mutations"
/db_xref="GeneID:853451"
/db_xref="SGD:S000006650"
gene <425157..>431237
/gene="CYR1"
/locus_tag="YJL005W"
/gene_synonym="CDC35; FIL1; HSR1; SRA4; TSM0185"
/db_xref="GeneID:853452"
mRNA <425157..>431237
/gene="CYR1"
/locus_tag="YJL005W"
/gene_synonym="CDC35; FIL1; HSR1; SRA4; TSM0185"
/product="adenylate cyclase"
/transcript_id="NM_001181439.3"
/db_xref="GeneID:853452"
CDS 425157..431237
/gene="CYR1"
/locus_tag="YJL005W"
/gene_synonym="CDC35; FIL1; HSR1; SRA4; TSM0185"
/EC_number="4.6.1.1"
/experiment="EXISTENCE:direct assay:GO:0004016 adenylate
cyclase activity [PMID:2934138]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:22575457|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:22575457]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:2196438]"
/experiment="EXISTENCE:genetic interaction:GO:0007188
adenylate cyclase-modulating G protein-coupled receptor
signaling pathway [PMID:2934138]"
/experiment="EXISTENCE:genetic interaction:GO:0007265 Ras
protein signal transduction [PMID:2934138]"
/experiment="EXISTENCE:mutant phenotype:GO:0004016
adenylate cyclase activity [PMID:6360999]"
/note="Adenylate cyclase; required for cAMP production and
cAMP-dependent protein kinase signaling; scaffold protein
for Ras2-Ira interaction; the cAMP pathway controls a
variety of cellular processes, including metabolism, cell
cycle, stress response, stationary phase, and sporulation"
/codon_start=1
/product="adenylate cyclase"
/protein_id="NP_012529.3"
/db_xref="GeneID:853452"
/db_xref="SGD:S000003542"
/translation="MSSKPDTGSEISGPQRQEEQEQQIEQSSPTEANDRSIHDEVPKV
KKRHEQNSGHKSRRNSAYSYYSPRSLSMTKSRESITPNGMDDVSISNVEHPRPTEPKI
KRGPYLLKKTLSSLSMTSANSTHDDNKDHGYALNSSKTHNYTSTHNHHDGHHDHHHVQ
FFPNRKPSLAETLFKRFSGSNSHDGNKSGKESKVANLSLSTVNPAPANRKPSKDSTLS
NHLADNVPSTLRRKVSSLVRGSSVHDINNGIADKQIRPKAVAQSENTLHSSDVPNSKR
SHRKSFLLGSTSSSSSRRGSNVSSMTNSDSASMATSGSHVLQHNVSNVSPTTKSKDSV
NSESADHTNNKSEKVTPEYNENIPENSNSDNKREATTPTIETPISCKPSLFRLDTNLE
DVTDITKTVPPTAVNSTLNSTHGTETASPKTVIMPEGPRKSVSMADLSVAAAAPNGEF
TSTSNDRSQWVAPQSWDVETKRKKTKPKGRSKSRRSSIDADELDPMSPGPPSKKDSRH
HHDRKDNESMVTAGDSNSSFVDICKENVPNDSKTALDTKSVNRLKSNLAMSPPSIRYA
PSNLDGDYDTSSTSSSLPSSSISSEDTSSCSDSSSYTNAYMEANREQDNKTPILNKTK
SYTKKFTSSSVNMNSPDGAQSSGLLLQDEKDDEVECQLEHYYKDFSDLDPKRHYAIRI
FNTDDTFTTLSCTPATTVEEIIPALKRKFNITAQGNFQISLKVGKLSKILRPTSKPIL
IERKLLLLNGYRKSDPLHIMGIEDLSFVFKFLFHPVTPSHFTPEQEQRIMRSEFVHVD
LRNMDLTTPPIIFYQHTSEIESLDVSNNANIFLPLEFIESSIKLLSLRMVNIRASKFP
SNITKAYKLVSLELQRNFIRKVPNSIMKLSNLTILNLQCNELESLPAGFVELKNLQLL
DLSSNKFMHYPEVINYCTNLLQIDLSYNKIQSLPQSTKYLVKLAKMNLSHNKLNFIGD
LSEMTDLRTLNLRYNRISSIKTNASNLQNLFLTDNRISNFEDTLPKLRALEIQENPIT
SISFKDFYPKNMTSLTLNKAQLSSIPGELLTKLSFLEKLELNQNNLTRLPQEISKLTK
LVFLSVARNKLEYIPPELSQLKSLRTLDLHSNNIRDFVDGMENLELTSLNISSNAFGN
SSLENSFYHNMSYGSKLSKSLMFFIAADNQFDDAMWPLFNCFVNLKVLNLSYNNFSDV
SHMKLESITELYLSGNKLTTLSGDTVLKWSSLKTLMLNSNQMLSLPAELSNLSQLSVF
DVGANQLKYNISNYHYDWNWRNNKELKYLNFSGNRRFEIKSFISHDIDADLSDLTVLP
QLKVLGLMDVTLNTTKVPDENVNFRLRTTASIINGMRYGVADTLGQRDYVSSRDVTFE
RFRGNDDECLLCLHDSKNQNADYGHNISRIVRDIYDKILIRQLERYGDETDDNIKTAL
RFSFLQLNKEINGMLNSVDNGADVANLSYADLLSGACSTVIYIRGKKLFAANLGDCMA
ILSKNNGDYQTLTKQHLPTKREEYERIRISGGYVNNGKLDGVVDVSRAVGFFDLLPHI
HASPDISVVTLTKADEMLIVATHKLWEYMDVDTVCDIARENSTDPLRAAAELKDHAMA
YGCTENITILCLALYENIQQQNRFTLNKNSLMTRRSTFEDTTLRRLQPEISPPTGNLA
MVFTDIKSSTFLWELFPNAMRTAIKTHNDIMRRQLRIYGGYEVKTEGDAFMVAFPTPT
SGLTWCLSVQLKLLDAQWPEEITSVQDGCQVTDRNGNIIYQGLSVRMGIHWGCPVPEL
DLVTQRMDYLGPMVNKAARVQGVADGGQIAMSSDFYSEFNKIMKYHERVVKGKESLKE
VYGEEIIGEVLEREIAMLESIGWAFFDFGEHKLKGLETKELVTIAYPKILASRHEFAS
EDEQSKLINETMLFRLRVISNRLESIMSALSGGFIELDSRTEGSYIKFNPKVENGIMQ
SISEKDALLFFDHVITRIESSVALLHLRQQRCSGLEICRNDKTSARSNIFNVVDELLQ
MVKNAKDLST"
gene complement(<431589..>432200)
/gene="SYS1"
/locus_tag="YJL004C"
/db_xref="GeneID:853453"
mRNA complement(<431589..>432200)
/gene="SYS1"
/locus_tag="YJL004C"
/product="Sys1p"
/transcript_id="NM_001181438.1"
/db_xref="GeneID:853453"
CDS complement(431589..432200)
/gene="SYS1"
/locus_tag="YJL004C"
/experiment="EXISTENCE:direct assay:GO:0000139 Golgi
membrane [PMID:11726510]"
/experiment="EXISTENCE:direct assay:GO:0005802 trans-Golgi
network [PMID:15077113]"
/experiment="EXISTENCE:genetic interaction:GO:0006895
Golgi to endosome transport [PMID:8923208]"
/experiment="EXISTENCE:mutant phenotype:GO:0034067 protein
localization to Golgi apparatus
[PMID:15077113|PMID:15077114]"
/experiment="EXISTENCE:mutant phenotype:GO:0043001 Golgi
to plasma membrane protein transport [PMID:16926193]"
/note="Integral membrane protein of the Golgi; required
for targeting of the Arf-like GTPase Arl3p to the Golgi;
multicopy suppressor of ypt6 null mutation"
/codon_start=1
/product="Sys1p"
/protein_id="NP_012530.1"
/db_xref="GeneID:853453"
/db_xref="SGD:S000003541"
/translation="MVSIRRYLRVPNELKPSQIFKQDSLSPSKIGLQIVLLQIFYYTT
AIVLFYCWAKLAGYDLNIKEWLFSWENIDFTNAYGLSISLLWLLDSLICVFFLTVIVG
RSKLAWDFAITIHAINFIVVFLYTRKFPSFSWFFLQILSSLILIFLGTWTTRWRELRD
TFFEGLVDPNEGEVGLVTPSQQHSNHSELEQSPIQLKDLESQI"
gene <432644..>433000
/gene="COX16"
/locus_tag="YJL003W"
/db_xref="GeneID:853454"
mRNA <432644..>433000
/gene="COX16"
/locus_tag="YJL003W"
/product="Cox16p"
/transcript_id="NM_001181437.1"
/db_xref="GeneID:853454"
CDS 432644..433000
/gene="COX16"
/locus_tag="YJL003W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:12446688]"
/experiment="EXISTENCE:mutant phenotype:GO:0033617
mitochondrial respiratory chain complex IV assembly
[PMID:12446688]"
/note="Mitochondrial inner membrane protein; required for
assembly of cytochrome c oxidase"
/codon_start=1
/product="Cox16p"
/protein_id="NP_012531.1"
/db_xref="GeneID:853454"
/db_xref="SGD:S000003540"
/translation="MSFSGKKFRSRRQQLVYEASLAGRYKKALSKHPFLFFGLPFCAT
IVLGSFWLSSFTAIKYEQGDRKVQEINEEDILKIRKNQREFDIKEEYYRLQGLSEEDW
EPVRVARLKDESENVW"
gene complement(<433221..>434651)
/gene="OST1"
/locus_tag="YJL002C"
/gene_synonym="NLT1"
/db_xref="GeneID:853455"
mRNA complement(<433221..>434651)
/gene="OST1"
/locus_tag="YJL002C"
/gene_synonym="NLT1"
/product="dolichyl-diphosphooligosaccharide--protein
glycotransferase subunit OST1"
/transcript_id="NM_001181436.3"
/db_xref="GeneID:853455"
CDS complement(433221..434651)
/gene="OST1"
/locus_tag="YJL002C"
/gene_synonym="NLT1"
/experiment="EXISTENCE:direct assay:GO:0005198 structural
molecule activity [PMID:29301962]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0006487 protein
N-linked glycosylation [PMID:9988747|PMID:9015380]"
/experiment="EXISTENCE:direct assay:GO:0006488
dolichol-linked oligosaccharide biosynthetic process
[PMID:29301962]"
/experiment="EXISTENCE:direct assay:GO:0008250
oligosaccharyltransferase complex [PMID:10358084]"
/experiment="EXISTENCE:physical interaction:GO:0008250
oligosaccharyltransferase complex [PMID:9405463]"
/note="Alpha subunit of the oligosaccharyltransferase
complex of the ER lumen; complex catalyzes
asparagine-linked glycosylation of newly synthesized
proteins"
/codon_start=1
/product="dolichyl-diphosphooligosaccharide--protein
glycotransferase subunit OST1"
/protein_id="NP_012532.3"
/db_xref="GeneID:853455"
/db_xref="SGD:S000003539"
/translation="MRQVWFSWIVGLFLCFFNVSSAAQYEPPATWENVDYKRTIDVSN
AYISETIEITIKNIASEPATEYFTAFESGIFSKVSFFSAYFTNEATFLNSQLLANSTT
APGDDGESEIRYGIIQFPNAISPQEEVSLVIKSFYNTVGIPYPEHVGMSEEQHLLWET
NRLPLSAYDTKKASFTLIGSSSFEEYHPPNDESLLGKANGNSFEFGPWEDIPRFSSNE
TLAIVYSHNAPLNQVVNLRRDIWLSHWASTIQFEEYYELTNKAAKLSKGFSRLELMKQ
IQTQNMRQTHFVTVLDMLLPEGATDHYFTDLVGLVSTSHAERDHFFIRPRFPIFGGWN
YNFTVGWTNKLSDFLHVSSGSDEKFVASIPILNGPPDTVYDNVELSVFLPEGAEIFDI
DSPVPFTNVSIETQKSYFDLNKGHVKLTFSYRNLISQVANGQVLIKYDYPKSSFFKKP
LSIACYIFTALMGVFVLKTLNMNVTN"
gene <435163..>435926
/gene="PRE3"
/locus_tag="YJL001W"
/gene_synonym="CRL21"
/db_xref="GeneID:853456"
mRNA join(<435163..435227,435344..>435926)
/gene="PRE3"
/locus_tag="YJL001W"
/gene_synonym="CRL21"
/product="proteasome core particle subunit beta 1"
/transcript_id="NM_001181435.1"
/db_xref="GeneID:853456"
CDS join(435163..435227,435344..435926)
/gene="PRE3"
/locus_tag="YJL001W"
/gene_synonym="CRL21"
/EC_number="3.4.25.1"
/experiment="EXISTENCE:direct assay:GO:0010499 proteasomal
ubiquitin-independent protein catabolic process
[PMID:19162040]"
/experiment="EXISTENCE:direct assay:GO:0019774 proteasome
core complex, beta-subunit complex [PMID:9087403]"
/experiment="EXISTENCE:direct assay:GO:0034515 proteasome
storage granule [PMID:18504300]"
/experiment="EXISTENCE:direct assay:GO:0043161
proteasome-mediated ubiquitin-dependent protein catabolic
process [PMID:11545745|PMID:19029916]"
/experiment="EXISTENCE:mutant phenotype:GO:0004175
endopeptidase activity [PMID:9312134|PMID:9207060]"
/note="Beta 1 subunit of the 20S proteasome; responsible
for cleavage after acidic residues in peptides"
/codon_start=1
/product="proteasome core particle subunit beta 1"
/protein_id="NP_012533.1"
/db_xref="GeneID:853456"
/db_xref="SGD:S000003538"
/translation="MNGIQVDINRLKKGEVSLGTSIMAVTFKDGVILGADSRTTTGAY
IANRVTDKLTRVHDKIWCCRSGSAADTQAIADIVQYHLELYTSQYGTPSTETAASVFK
ELCYENKDNLTAGIIVAGYDDKNKGEVYTIPLGGSVHKLPYAIAGSGSTFIYGYCDKN
FRENMSKEETVDFIKHSLSQAIKWDGSSGGVIRMVVLTAAGVERLIFYPDEYEQL"
centromere complement(436307..436425)
/note="CEN10; Chromosome X centromere"
/db_xref="SGD:S000006471"
centromere complement(436307..436331)
/note="CEN10_CDEIII of CEN10"
centromere complement(436332..436415)
/note="CEN10_CDEII of CEN10"
centromere complement(436416..436425)
/note="CEN10_CDEI of CEN10"
gene <436802..>438610
/gene="AVT1"
/locus_tag="YJR001W"
/db_xref="GeneID:853457"
mRNA <436802..>438610
/gene="AVT1"
/locus_tag="YJR001W"
/product="Avt1p"
/transcript_id="NM_001181658.1"
/db_xref="GeneID:853457"
CDS 436802..438610
/gene="AVT1"
/locus_tag="YJR001W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:11274162|PMID:27246536]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0000329
fungal-type vacuole membrane [PMID:11274162]"
/experiment="EXISTENCE:mutant phenotype:GO:0003333 amino
acid transmembrane transport [PMID:11274162]"
/experiment="EXISTENCE:mutant phenotype:GO:0005302
L-tyrosine transmembrane transporter activity
[PMID:11274162]"
/experiment="EXISTENCE:mutant phenotype:GO:0006865 amino
acid transport [PMID:11274162]"
/experiment="EXISTENCE:mutant phenotype:GO:0007034
vacuolar transport [PMID:11274162]"
/experiment="EXISTENCE:mutant phenotype:GO:0015186
L-glutamine transmembrane transporter activity
[PMID:11274162]"
/experiment="EXISTENCE:mutant phenotype:GO:0015188
L-isoleucine transmembrane transporter activity
[PMID:11274162]"
/experiment="EXISTENCE:mutant phenotype:GO:0015824 proline
transport [PMID:27246536]"
/note="Vacuolar transporter; imports large neutral amino
acids into vacuole; targeted to vacuole via AP-3 pathway;
member of family of seven S. cerevisiae genes (AVT1-7)
related to vesicular GABA-glycine transporters"
/codon_start=1
/product="Avt1p"
/protein_id="NP_012534.1"
/db_xref="GeneID:853457"
/db_xref="SGD:S000003761"
/translation="MPEQEPLSPNGRKRSEVHYISIPLNRGSAFSPDDSVSQFQSDGF
MTRRQSILDHPVGSFKGVNSLSRFATSLRRANSFRNIELNADNERSFFKESNDETYDP
DTLAPALDGRRLSVTLNNAGRPRITNLANNDRVSTASMAIHDDDYGSIQNSTIGDSGS
ILRPTASLTEMMSGGAGRRFTNNDMDSIVVKRVEGVDGKVVTLLAGQSTAPQTIFNSI
NVLIGIGLLALPLGLKYAGWVIGLTMLAIFALATFCTAELLSRCLDTDPTLISYADLG
YAAFGTKGRALISALFTLDLLGSGVSLVILFGDSLNALFPQYSTTFFKIVSFFIVTPP
VFIPLSVLSNISLLGILSTTGTVLVICCCGLYKSSSPGSLVNPMETSMWPIDLKHLCL
SIGLLSACWGGHAVFPNLKTDMRHPDKFKDCLKTTYKITSVTDIGTAVIGFLMFGNLV
KDEITKNVLLTEGYPKFVYGLISALMTIIPIAKTPLNARPIVSVLDVLMNVQHIDEAA
SAIKRRAAKGLQVFNRIFINVVFVLIAINFPEFDKIIAFLGAGLCFTICLILPCWFYL
RLCKTTIKPWERVACHVTICISVVLSTLGVGAAIIS"
gene <438865..>440646
/gene="MPP10"
/locus_tag="YJR002W"
/db_xref="GeneID:853458"
mRNA <438865..>440646
/gene="MPP10"
/locus_tag="YJR002W"
/product="rRNA-processing protein MPP10"
/transcript_id="NM_001181659.3"
/db_xref="GeneID:853458"
CDS 438865..440646
/gene="MPP10"
/locus_tag="YJR002W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/experiment="EXISTENCE:direct assay:GO:0032040
small-subunit processome [PMID:12068309]"
/experiment="EXISTENCE:direct assay:GO:0034457 Mpp10
complex [PMID:17515605]"
/experiment="EXISTENCE:mutant phenotype:GO:0000447
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from
5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:9315638]"
/experiment="EXISTENCE:mutant phenotype:GO:0000472
endonucleolytic cleavage to generate mature 5'-end of
SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:9315638]"
/experiment="EXISTENCE:mutant phenotype:GO:0000480
endonucleolytic cleavage in 5'-ETS of tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:9315638]"
/experiment="EXISTENCE:mutant phenotype:GO:0032040
small-subunit processome [PMID:12242301]"
/note="Component of the SSU processome and 90S
preribosome; required for pre-18S rRNA processing,
interacts with and controls the stability of Imp3p and
Imp4p, essential for viability; similar to human Mpp10p"
/codon_start=1
/product="rRNA-processing protein MPP10"
/protein_id="NP_012535.3"
/db_xref="GeneID:853458"
/db_xref="SGD:S000003762"
/translation="MSELFGVLKSNAGRIILKDPSATSKDVKAYIDSVINTCKNGSIT
KKAELDEITVDGLDANQVWWQVKLVLDSIDGDLIQGIQELKDVVTPSHNLSDGSTLNS
SSGEESELEEAESVFKEKQMLSADVSEIEEQSNDSLSENDEEPSMDDEKTSAEAAREE
FAEEKRISSGQDERHSSPDPYGINDKFFDLEKFNRDTLAAEDSNEASEGSEDEDIDYF
QDMPSDDEEEEAIYYEDFFDKPTKEPVKKHSDVKDPKEDEELDEEEHDSAMDKVKLDL
FADEEDEPNAEGVGEASDKNLSSFEKQQIEIRKQIEQLENEAVAEKKWSLKGEVKAKD
RPEDALLTEELEFDRTAKPVPVITSEVTESLEDMIRRRIQDSNFDDLQRRTLLDITRK
SQRPQFELSDVKSSKSLAEIYEDDYTRAEDESALSEELQKAHSEISELYANLVYKLDV
LSSVHFVPKPASTSLEIRVETPTISMEDAQPLYMSNASSLAPQEIYNVGKAEKDGEIR
LKNGVAMSKEELTREDKNRLRRALKRKRSKANLPNVNKRSKRNDVVDTLSKAKNITVI
NQKGEKKDVSGKTKKSRSGPDSTNIKL"
gene complement(<440994..>442553)
/gene="MRX12"
/locus_tag="YJR003C"
/db_xref="GeneID:853459"
mRNA complement(<440994..>442553)
/gene="MRX12"
/locus_tag="YJR003C"
/product="Mrx12p"
/transcript_id="NM_001181660.1"
/db_xref="GeneID:853459"
CDS complement(440994..442553)
/gene="MRX12"
/locus_tag="YJR003C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961|PMID:14562095]"
/note="Protein that associates with mitochondrial
ribosome; detected in highly purified mitochondria in
high-throughput studies; predicted to be involved in
ribosome biogenesis"
/codon_start=1
/product="Mrx12p"
/protein_id="NP_012536.2"
/db_xref="GeneID:853459"
/db_xref="SGD:S000003763"
/translation="MLRSLHSAATLSNKRFYSLISHSNRKNIIKKLLRHPSFDPIRHH
LPEDITTIDPYSLSQNVIESLNKLEVPKKDAAMVHNMMIENLSDLDYGVATIHSNNLR
DLDLKPSLPAIKQIIRNNPGRVQSSWELFTQYKASMENVPDELMEVVLEKIIKFDKAE
KVDGKKSLTYQDLVRCLYLINHFSSNYNLPSELVEPILIYIVDNGIPNVLGSVLKYKI
PLSFFDKYVSEMTQYQICELYDFYSLDNIVADPLVLHKCLTVLGENEKIQQTEEEKEI
ISKLEEEIDIVKSQCHDNWSLEFPNWSVRKTATSFEELFLEIQKRNIDKKDFELAHKL
LRLIGAFKGKVSLFFKLYDEYLLKFKNNEDDLMFEAFLTLCCQGYKSSNEKMLQYAEA
FIKEDFDSKLESKIQSVLIVANAKANIDLSLKIYNSNISTAKREKDKYTDLAESDVLT
ESLILAFLSRDDADFARVIFDGALGEKLISGPTAAKKIKNLLAQYGEALETKTSKQVM
QTKIEHYMESI"
rep_origin 442556..442965
/note="ARS1015; Autonomously Replicating Sequence"
/db_xref="SGD:S000028452"
gene complement(<442909..>444861)
/gene="SAG1"
/locus_tag="YJR004C"
/gene_synonym="AG(ALPHA)1"
/db_xref="GeneID:853460"
mRNA complement(<442909..>444861)
/gene="SAG1"
/locus_tag="YJR004C"
/gene_synonym="AG(ALPHA)1"
/product="Sag1p"
/transcript_id="NM_001181661.1"
/db_xref="GeneID:853460"
CDS complement(442909..444861)
/gene="SAG1"
/locus_tag="YJR004C"
/gene_synonym="AG(ALPHA)1"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:10383953]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0000752
agglutination involved in conjugation with cellular fusion
[PMID:2677666|PMID:2673555]"
/experiment="EXISTENCE:mutant phenotype:GO:0050839 cell
adhesion molecule binding
[PMID:11369849|PMID:2677666|PMID:2673555]"
/note="Alpha-agglutinin of alpha-cells; binds to Aga1p
during agglutination, N-terminal half is homologous to the
immunoglobulin superfamily and contains binding site for
a-agglutinin, C-terminal half is highly glycosylated and
contains GPI anchor"
/codon_start=1
/product="Sag1p"
/protein_id="NP_012537.1"
/db_xref="GeneID:853460"
/db_xref="SGD:S000003764"
/translation="MFTFLKIILWLFSLALASAININDITFSNLEITPLTANKQPDQG
WTATFDFSIADASSIREGDEFTLSMPHVYRIKLLNSSQTATISLADGTEAFKCYVSQQ
AAYLYENTTFTCTAQNDLSSYNTIDGSITFSLNFSDGGSSYEYELENAKFFKSGPMLV
KLGNQMSDVVNFDPAAFTENVFHSGRSTGYGSFESYHLGMYCPNGYFLGGTEKIDYDS
SNNNVDLDCSSVQVYSSNDFNDWWFPQSYNDTNADVTCFGSNLWITLDEKLYDGEMLW
VNALQSLPANVNTIDHALEFQYTCLDTIANTTYATQFSTTREFIVYQGRNLGTASAKS
SFISTTTTDLTSINTSAYSTGSISTVETGNRTTSEVISHVVTTSTKLSPTATTSLTIA
QTSIYSTDSNITVGTDIHTTSEVISDVETISRETASTVVAAPTSTTGWTGAMNTYISQ
FTSSSFATINSTPIISSSAVFETSDASIVNVHTENITNTAAVPSEEPTFVNATRNSLN
SFCSSKQPSSPSSYTSSPLVSSLSVSKTLLSTSFTPSVPTSNTYIKTKNTGYFEHTAL
TTSSVGLNSFSETAVSSQGTKIDTFLVSSLIAYPSSASGSQLSGIQQNFTSTSLMIST
YEGKASIFFSAELGSIIFLLLSYLLF"
gene <445923..>448025
/gene="APL1"
/locus_tag="YJR005W"
/gene_synonym="YAP80"
/db_xref="GeneID:853461"
mRNA <445923..>448025
/gene="APL1"
/locus_tag="YJR005W"
/gene_synonym="YAP80"
/product="Apl1p"
/transcript_id="NM_001181662.3"
/db_xref="GeneID:853461"
CDS 445923..448025
/gene="APL1"
/locus_tag="YJR005W"
/gene_synonym="YAP80"
/experiment="EXISTENCE:curator inference:GO:0016192
vesicle-mediated transport [PMID:22190733]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0030122 AP-2
adaptor complex [PMID:10564262]"
/experiment="EXISTENCE:direct assay:GO:0030139 endocytic
vesicle [PMID:22190733]"
/note="Beta-adaptin; large subunit of the clathrin
associated protein complex (AP-2); involved in vesicle
mediated transport; similar to mammalian beta-chain of the
clathrin associated protein complex"
/codon_start=1
/product="Apl1p"
/protein_id="NP_012538.3"
/db_xref="GeneID:853461"
/db_xref="SGD:S000003765"
/translation="MSDQKVFARYKANEIVTDLQHFGVKKFKSNITRRKNALRKIIAN
LVLGNYGEMSVLFSELLKFWQIEDDLEVKRICHEYIRVIGALKPQQAREALPFIMDDF
KSRDEKLQIMALRTLVLVPVKELSDQAFDCIISLVNHKSPPEQVTRTAIYALLDLDEI
DHERVLGLSSILHDIVKAQSSSPEVIVAALHTLYSIHEKNANMEPFRIPLELAFDMLE
LLPELNEWNKATVLEVLTTSVVPQHYLDTHEMIELALPYLQQVNTYVVLNSLKFIMYL
LNYVDVIKETLAEKLSNSVIALLDKPPELQFLVLRNVILLLLSRESSLLRLDISYFFI
EYNDPIYIKDTKLECLYLLANKETLPRILEELEQYATDIDIQMSRKSVRAIGNLAVKL
DEDSVHDCVAVLLDLLEFGVDYVVQEIISVFRNILRKYPNNFKANVTELVKHTEVVQE
PESKNAMIWIITQYSDVIPNYLELFRVFSSNMFSETLEVQFSILNSAIKFFIRSPTKE
TEELCMDLLKGCIDHENNPDLRDKTLMYWRLLSLTKTSRISNAITFESLKSVLDGELP
LIEMNTKLDPTVLEELELNIGTIVSIYLKPVSHIFRLNKTKLLPQSPILNPNKDLLPV
VGNSFPPTGANRDRQNSESQSSTKSRKTAMMDDYDKPAEKINQLKGKRKSSSNNPSKL
SRKPSTLLRKLSMKRPFS"
gene complement(<448477..>448758)
/gene="LSO1"
/locus_tag="YJR005C-A"
/db_xref="GeneID:1466469"
mRNA complement(<448477..>448758)
/gene="LSO1"
/locus_tag="YJR005C-A"
/product="Lso1p"
/transcript_id="NM_001184533.1"
/db_xref="GeneID:1466469"
CDS complement(448477..448758)
/gene="LSO1"
/locus_tag="YJR005C-A"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:26450372]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:26450372]"
/experiment="EXISTENCE:mutant phenotype:GO:0006879
intracellular iron ion homeostasis [PMID:26450372]"
/note="Protein with a potential role in response to iron
deprivation; transcription increases during iron
deprivation and during treatment with
2-(6-benzyl-2-pyridyl)quinazoline (BPQ) and copper;
regulated by Aft1p and, to a lesser extent, by Aft2p;
originally identified as a syntenic homolog of an Ashbya
gossypii gene; localizes to nucleus and cytoplasm, and
nuclear localization is enhanced under iron-replete
conditions"
/codon_start=1
/product="Lso1p"
/protein_id="NP_878106.1"
/db_xref="GeneID:1466469"
/db_xref="SGD:S000028523"
/translation="MHNTGKRYSETAKKVAAGRARKRRQAYEKDQLEKQQLEAQEAQR
WEEGARTPNQKKLIMEQKKTEKLRAKKERDQLLAAEEEALGKGGRGKRY"
gene <449202..>450665
/gene="POL31"
/locus_tag="YJR006W"
/gene_synonym="HUS2; HYS2; SDP5"
/db_xref="GeneID:853462"
mRNA <449202..>450665
/gene="POL31"
/locus_tag="YJR006W"
/gene_synonym="HUS2; HYS2; SDP5"
/product="DNA-directed DNA polymerase delta subunit POL31"
/transcript_id="NM_001181663.1"
/db_xref="GeneID:853462"
CDS 449202..450665
/gene="POL31"
/locus_tag="YJR006W"
/gene_synonym="HUS2; HYS2; SDP5"
/EC_number="2.7.7.7"
/experiment="EXISTENCE:direct assay:GO:0003887
DNA-directed DNA polymerase activity [PMID:18635534]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0006278
RNA-templated DNA biosynthetic process [PMID:17429354]"
/experiment="EXISTENCE:direct assay:GO:0016035 zeta DNA
polymerase complex [PMID:23066099|PMID:22711820]"
/experiment="EXISTENCE:direct assay:GO:0043137 DNA
replication, removal of RNA primer [PMID:16837458]"
/note="Subunit of DNA polymerase delta (polymerase III);
essential for cell viability; involved in DNA replication
and DNA repair; forms a complex with Rev3p, Rev7p and
Pol32p; relocalizes to the cytosol in response to hypoxia"
/codon_start=1
/product="DNA-directed DNA polymerase delta subunit POL31"
/protein_id="NP_012539.1"
/db_xref="GeneID:853462"
/db_xref="SGD:S000003766"
/translation="MDALLTKFNEDRSLQDENLSQPRTRVRIVDDNLYNKSNPFQLCY
KKRDYGSQYYHIYQYRLKTFRERVLKECDKRWDAGFTLNGQLVLKKDKVLDIQGNQPC
WCVGSIYCEMKYKPNVLDEVINDTYGAPDLTKSYTDKEGGSDEIMLEDESGRVLLVGD
FIRSTPFITGVVVGILGMEAEAGTFQVLDICYPTPLPQNPFPAPIATCPTRGKIALVS
GLNLNNTSPDRLLRLEILREFLMGRINNKIDDISLIGRLLICGNSVDFDIKSVNKDEL
MISLTEFSKFLHNILPSISVDIMPGTNDPSDKSLPQQPFHKSLFDKSLESYFNGSNKE
ILNLVTNPYEFSYNGVDVLAVSGKNINDICKYVIPSNDNGESENKVEEGESNDFKDDI
EHRLDLMECTMKWQNIAPTAPDTLWCYPYTDKDPFVLDKWPHVYIVANQPYFGTRVVE
IGGKNIKIISVPEFSSTGMIILLDLETLEAETVKIDI"
gene <451020..>451934
/gene="SUI2"
/locus_tag="YJR007W"
/db_xref="GeneID:853463"
mRNA <451020..>451934
/gene="SUI2"
/locus_tag="YJR007W"
/product="translation initiation factor eIF2 subunit
alpha"
/transcript_id="NM_001181664.3"
/db_xref="GeneID:853463"
CDS 451020..451934
/gene="SUI2"
/locus_tag="YJR007W"
/experiment="EXISTENCE:direct assay:GO:0001731 formation
of translation preinitiation complex [PMID:12008673]"
/experiment="EXISTENCE:direct assay:GO:0003743 translation
initiation factor activity [PMID:16565414]"
/experiment="EXISTENCE:direct assay:GO:0005525 GTP binding
[PMID:14698289|PMID:16246727]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0006413
translational initiation [PMID:8947054]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405]"
/experiment="EXISTENCE:direct assay:GO:0033290 eukaryotic
48S preinitiation complex [PMID:17242201]"
/experiment="EXISTENCE:direct assay:GO:0043614 multi-eIF
complex [PMID:11018020|PMID:15838098]"
/experiment="EXISTENCE:direct assay:GO:1990856
methionyl-initiator methionine tRNA binding
[PMID:16565414]"
/experiment="EXISTENCE:mutant phenotype:GO:0005850
eukaryotic translation initiation factor 2 complex
[PMID:2649894]"
/note="Alpha subunit of the translation initiation factor
eIF2; eIF2 is involved in identification of the start
codon; phosphorylation of Ser51 is required for regulation
of translation by inhibiting the exchange of GDP for GTP;
protein abundance increases in response to DNA replication
stress"
/codon_start=1
/product="translation initiation factor eIF2 subunit
alpha"
/protein_id="NP_012540.3"
/db_xref="GeneID:853463"
/db_xref="SGD:S000003767"
/translation="MSTSHCRFYENKYPEIDDIVMVNVQQIAEMGAYVKLLEYDNIEG
MILLSELSRRRIRSIQKLIRVGKNDVAVVLRVDKEKGYIDLSKRRVSSEDIIKCEEKY
QKSKTVHSILRYCAEKFQIPLEELYKTIAWPLSRKFGHAYEAFKLSIIDETVWEGIEP
PSKDVLDELKNYISKRLTPQAVKIRADVEVSCFSYEGIDAIKDALKSAEDMSTEQMQV
KVKLVAAPLYVLTTQALDKQKGIEQLESAIEKITEVITKYGGVCNITMPPKAVTATED
AELQALLESKELDNRSDSEDDEDESDDE"
gene <452430..>453446
/gene="MHO1"
/locus_tag="YJR008W"
/db_xref="GeneID:853464"
mRNA <452430..>453446
/gene="MHO1"
/locus_tag="YJR008W"
/product="Mho1p"
/transcript_id="NM_001181665.1"
/db_xref="GeneID:853464"
CDS 452430..453446
/gene="MHO1"
/locus_tag="YJR008W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22412880|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:22412880]"
/note="hypothetical protein; inhibits haploid invasive
growth when overexpressed; synthetically lethal with
phospholipase C (PLC1); expression induced by mild
heat-stress on a non-fermentable carbon source, upon entry
into stationary phase and upon nitrogen deprivation;
repressed by inosine and choline in an Opi1p-dependent
manner; highly conserved from bacteria to human; Memo, the
human homolog, is an ErbB2 interacting protein with an
essential function in cell motility"
/codon_start=1
/product="Mho1p"
/protein_id="NP_012541.1"
/db_xref="GeneID:853464"
/db_xref="SGD:S000003768"
/translation="MSIRPATHAGSWYSNRAQELSQQLHTYLIKSTLKGPIHNARIII
CPHAGYRYCGPTMAYSYASLDLNRNVKRIFILGPSHHIYFKNQILVSAFSELETPLGN
LKVDTDLCKTLIQKEYPENGKKLFKPMDHDTDMAEHSLEMQLPMLVETLKWREISLDT
VKVFPMMVSHNSVDVDRCIGNILSEYIKDPNNLFIVSSDFCHWGRRFQYTGYVGSKEE
LNDAIQEETEVEMLTARSKLSHHQVPIWQSIEIMDRYAMKTLSDTPNGERYDAWKQYL
EITGNTICGEKPISVILSALSKIRDAGPSGIKFQWPNYSQSSHVTSIDDSSVSYASGY
VTIG"
gene complement(<453683..>454681)
/gene="TDH2"
/locus_tag="YJR009C"
/gene_synonym="GLD2"
/db_xref="GeneID:853465"
mRNA complement(<453683..>454681)
/gene="TDH2"
/locus_tag="YJR009C"
/gene_synonym="GLD2"
/product="glyceraldehyde-3-phosphate dehydrogenase
(phosphorylating) TDH2"
/transcript_id="NM_001181666.1"
/db_xref="GeneID:853465"
CDS complement(453683..454681)
/gene="TDH2"
/locus_tag="YJR009C"
/gene_synonym="GLD2"
/EC_number="1.2.1.12"
/experiment="EXISTENCE:direct assay:GO:0004365
glyceraldehyde-3-phosphate dehydrogenase (NAD+)
(phosphorylating) activity [PMID:3905788]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16962558]"
/experiment="EXISTENCE:direct assay:GO:0005811 lipid
droplet [PMID:10515935]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762|PMID:11158358]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:11158358]"
/experiment="EXISTENCE:direct assay:GO:1904408 melatonin
binding [PMID:31708896]"
/experiment="EXISTENCE:direct assay:GO:1990841
promoter-specific chromatin binding [PMID:27184763]"
/experiment="EXISTENCE:expression pattern:GO:0006094
gluconeogenesis [PMID:3905788]"
/experiment="EXISTENCE:expression pattern:GO:0006096
glycolytic process [PMID:3905788]"
/experiment="EXISTENCE:mutant phenotype:GO:0006915
apoptotic process [PMID:17726063]"
/experiment="EXISTENCE:mutant phenotype:GO:0072593
reactive oxygen species metabolic process [PMID:17726063]"
/note="Glyceraldehyde-3-phosphate dehydrogenase (GAPDH),
isozyme 2; involved in glycolysis and gluconeogenesis;
tetramer that catalyzes reaction of
glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate;
detected in cytoplasm and cell wall; protein abundance
increases in response to DNA replication stress;
GAPDH-derived antimicrobial peptides are active against a
wide variety of wine-related yeasts and bateria; TDH2 has
a paralog, TDH3, that arose from the whole genome
duplication"
/codon_start=1
/product="glyceraldehyde-3-phosphate dehydrogenase
(phosphorylating) TDH2"
/protein_id="NP_012542.1"
/db_xref="GeneID:853465"
/db_xref="SGD:S000003769"
/translation="MVRVAINGFGRIGRLVMRIALQRKNVEVVALNDPFISNDYSAYM
FKYDSTHGRYAGEVSHDDKHIIVDGHKIATFQERDPANLPWASLNIDIAIDSTGVFKE
LDTAQKHIDAGAKKVVITAPSSTAPMFVMGVNEEKYTSDLKIVSNASCTTNCLAPLAK
VINDAFGIEEGLMTTVHSMTATQKTVDGPSHKDWRGGRTASGNIIPSSTGAAKAVGKV
LPELQGKLTGMAFRVPTVDVSVVDLTVKLNKETTYDEIKKVVKAAAEGKLKGVLGYTE
DAVVSSDFLGDSNSSIFDAAAGIQLSPKFVKLVSWYDNEYGYSTRVVDLVEHVAKA"
rep_origin 454584..455555
/note="ARS1016; Autonomously Replicating Sequence"
/db_xref="SGD:S000028453"
gene <456239..>457774
/gene="MET3"
/locus_tag="YJR010W"
/db_xref="GeneID:853466"
mRNA <456239..>457774
/gene="MET3"
/locus_tag="YJR010W"
/product="sulfate adenylyltransferase"
/transcript_id="NM_001181668.3"
/db_xref="GeneID:853466"
CDS 456239..457774
/gene="MET3"
/locus_tag="YJR010W"
/EC_number="2.7.7.4"
/experiment="EXISTENCE:direct assay:GO:0004781 sulfate
adenylyltransferase (ATP) activity [PMID:10092498]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:mutant phenotype:GO:0000096 sulfur
amino acid metabolic process [PMID:1101032]"
/experiment="EXISTENCE:mutant phenotype:GO:0004781 sulfate
adenylyltransferase (ATP) activity [PMID:197388]"
/experiment="EXISTENCE:mutant phenotype:GO:0019379 sulfate
assimilation, phosphoadenylyl sulfate reduction by
phosphoadenylyl-sulfate reductase (thioredoxin)
[PMID:197388]"
/note="ATP sulfurylase; catalyzes the primary step of
intracellular sulfate activation, essential for
assimilatory reduction of sulfate to sulfide, involved in
methionine metabolism; human homolog PAPSS2 complements
yeast null mutant"
/codon_start=1
/product="sulfate adenylyltransferase"
/protein_id="NP_012543.3"
/db_xref="GeneID:853466"
/db_xref="SGD:S000003771"
/translation="MPAPHGGILQDLIARDALKKNELLSEAQSSDILVWNLTPRQLCD
IELILNGGFSPLTGFLNENDYSSVVTDSRLADGTLWTIPITLDVDEAFANQIKPDTRI
ALFQDDEIPIAILTVQDVYKPNKTIEAEKVFRGDPEHPAISYLFNVAGDYYVGGSLEA
IQLPQHYDYPGLRKTPAQLRLEFQSRQWDRVVAFQTRNPMHRAHRELTVRAAREANAK
VLIHPVVGLTKPGDIDHHTRVRVYQEIIKRYPNGIAFLSLLPLAMRMSGDREAVWHAI
IRKNYGASHFIVGRDHAGPGKNSKGVDFYGPYDAQELVESYKHELDIEVVPFRMVTYL
PDEDRYAPIDQIDTTKTRTLNISGTELRRRLRVGGEIPEWFSYPEVVKILRESNPPRP
KQGFSIVLGNSLTVSREQLSIALLSTFLQFGGGRYYKIFEHNNKTELLSLIQDFIGSG
SGLIIPNQWEDDKDSVVGKQNVYLLDTSSSADIQLESADEPISHIVQKVVLFLEDNGF
FVF"
gene complement(<458077..>458361)
/gene="SPC1"
/locus_tag="YJR010C-A"
/db_xref="GeneID:853467"
mRNA complement(<458077..>458361)
/gene="SPC1"
/locus_tag="YJR010C-A"
/product="signal peptidase complex subunit SPC1"
/transcript_id="NM_001181667.1"
/db_xref="GeneID:853467"
CDS complement(458077..458361)
/gene="SPC1"
/locus_tag="YJR010C-A"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005787 signal
peptidase complex [PMID:1846444|PMID:8910564]"
/experiment="EXISTENCE:genetic interaction:GO:0006465
signal peptide processing [PMID:8663399]"
/experiment="EXISTENCE:genetic interaction:GO:0045047
protein targeting to ER [PMID:8663399]"
/experiment="EXISTENCE:mutant phenotype:GO:0006465 signal
peptide processing [PMID:8663399]"
/experiment="EXISTENCE:physical interaction:GO:0045047
protein targeting to ER [PMID:8663399]"
/note="Subunit of the signal peptidase complex (SPC); SPC
cleaves the signal sequence from proteins targeted to the
endoplasmic reticulum (ER); regulates cellular levels of
membrane proteins; homolog of the SPC12 subunit of
mammalian signal peptidase complex; protein abundance
increases in response to DNA replication stress"
/codon_start=1
/product="signal peptidase complex subunit SPC1"
/protein_id="NP_012544.1"
/db_xref="GeneID:853467"
/db_xref="SGD:S000003770"
/translation="MSEILQDVQRKLVFPIDFPSQRKTEKFQQLSLMIGALVACILGF
AQQSLKVLLTAYGISCVITLICVLPAYPWYNKQKLRWAQPKIEINVDQYD"
gene complement(<458641..>459426)
/gene="CAL4"
/locus_tag="YJR011C"
/db_xref="GeneID:853468"
mRNA complement(<458641..>459426)
/gene="CAL4"
/locus_tag="YJR011C"
/product="Cal4p"
/transcript_id="NM_001181669.1"
/db_xref="GeneID:853468"
CDS complement(458641..459426)
/gene="CAL4"
/locus_tag="YJR011C"
/note="Accessory component of the Ccr4-NOT complex;
GFP-fusion protein expression is induced in response to
the DNA-damaging agent MMS"
/codon_start=1
/product="Cal4p"
/protein_id="NP_012545.1"
/db_xref="GeneID:853468"
/db_xref="SGD:S000003772"
/translation="MSETRESNESTVSSLQTKLLLNDGISENNKKNVILLNQIVPFIL
NTSHYMTDLMYVLYYLAQKQEDEVLNHSGTFISHKKELLALKSDICELIYDLRTGFRL
LLDSCELDHFETPGKCRHLIEKVLVTSIYGVNRYIFQELNRLNVDFKDEFILQMQNCL
SGFVNLYKFLNKIPMSKQQSQMNDLQMKILVNVLQNELLPIWKFQLDLLNCKLFNELS
KDKGLINIYRKATNDSVIDVSKGEPFIRYVNWLKDQIIGEMTV"
rep_origin 459337..459895
/note="ARS1017; Autonomously Replicating Sequence"
/db_xref="SGD:S000028454"
gene complement(<459795..>460193)
/gene="PEX39"
/locus_tag="YJR012C"
/db_xref="GeneID:853469"
mRNA complement(<459795..>460193)
/gene="PEX39"
/locus_tag="YJR012C"
/product="peroxin PEX39"
/transcript_id="NM_001181670.4"
/db_xref="GeneID:853469"
CDS complement(459795..460193)
/gene="PEX39"
/locus_tag="YJR012C"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:40739340]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:40739340]"
/experiment="EXISTENCE:genetic interaction:GO:0016558
protein import into peroxisome matrix [PMID:40739340]"
/experiment="EXISTENCE:mutant phenotype:GO:0016558 protein
import into peroxisome matrix [PMID:40739340]"
/experiment="EXISTENCE:physical interaction:GO:0030674
protein-macromolecule adaptor activity [PMID:40739340]"
/note="Cytosolic peroxin that facilitates the peroxisomal
import of PTS2-containing proteins; (R/K)PWE motif
containing protein that binds to Pex7p, stabilizing its
interaction with PTS2-containing cargo proteins; proposed
involvement in transport based on mass spectrometry
analysis of co-purifying proteins; ortholog of human
PEX39"
/codon_start=1
/product="peroxin PEX39"
/protein_id="NP_012546.4"
/db_xref="GeneID:853469"
/db_xref="SGD:S000003773"
/translation="MAAGGELSYEELLDHILNNKPIPNIVEVPNVTLDEGLASTPSLR
PRPRPWEGQLQHQSHQGSLDKPNISLDIDQESLEGMTSLTRLSECYDIQSKLQINDSD
NDNDDNNNDNNKGDGNDDDNNTVTANPTAR"
gene <460382..>461593
/gene="GPI14"
/locus_tag="YJR013W"
/gene_synonym="PMH1"
/db_xref="GeneID:853470"
mRNA <460382..>461593
/gene="GPI14"
/locus_tag="YJR013W"
/gene_synonym="PMH1"
/product="glycosylphosphatidylinositol-alpha 1,4
mannosyltransferase I"
/transcript_id="NM_001181671.1"
/db_xref="GeneID:853470"
CDS 460382..461593
/gene="GPI14"
/locus_tag="YJR013W"
/gene_synonym="PMH1"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0006506 GPI anchor
biosynthetic process [PMID:15635094]"
/experiment="EXISTENCE:direct assay:GO:0180041
dol-P-Man:GlcN-acyl-PI alpha-1,4-mannosyltransferase
activity [PMID:15635094]"
/experiment="EXISTENCE:mutant phenotype:GO:0006506 GPI
anchor biosynthetic process [PMID:16134120]"
/experiment="EXISTENCE:mutant phenotype:GO:0031505
fungal-type cell wall organization [PMID:16134120]"
/experiment="EXISTENCE:physical interaction:GO:0006506 GPI
anchor biosynthetic process [PMID:15635094]"
/experiment="EXISTENCE:physical interaction:GO:0031505
fungal-type cell wall organization [PMID:15635094]"
/experiment="EXISTENCE:physical interaction:GO:1990529
glycosylphosphatidylinositol-mannosyltransferase I complex
[PMID:15635094]"
/note="Glycosylphosphatidylinositol-alpha 1,4
mannosyltransferase I; involved in GPI anchor
biosynthesis, requires Pbn1p for function; homolog of
mammalian PIG-M"
/codon_start=1
/product="glycosylphosphatidylinositol-alpha 1,4
mannosyltransferase I"
/protein_id="NP_012547.2"
/db_xref="GeneID:853470"
/db_xref="SGD:S000003774"
/translation="MTGEEWGLTVLSFLVRVGFFLFGIYQDANFKVRYTDIDYFVFHD
AAKYVYEGKSPYARDTYRYTPLLSWLLVPNHYFGWFHLGKVIFVIFDLVTGLIIMKLL
NQAISRKRALILESIWLLNPMVITISTRGNAESVLCCLIMFTLFFLQKSRYTLAGILY
GLSIHFKIYPIIYCIPIAIFIYYNKRNQGPRTQLTSLLNIGLSTLTTLLGCGWAMYKI
YGYEFLDQAYLYHLYRTDHRHNFSVWNMLLYLDSANKENGESNLSRYAFVPQLLLVLV
TGCLEWWNPTFDNLLRVLFVQTFAFVTYNKVCTSQYFVWYLIFLPFYLSRTHIGWKKG
LLMATLWVGTQGIWLSQGYYLEFEGKNVFYPGLFIASVLFFVTNVWLLGQFITDIKIP
TQPTVSNKKNN"
gene <461829..>462425
/gene="TMA22"
/locus_tag="YJR014W"
/db_xref="GeneID:853471"
mRNA <461829..>462425
/gene="TMA22"
/locus_tag="YJR014W"
/product="Tma22p"
/transcript_id="NM_001181672.1"
/db_xref="GeneID:853471"
CDS 461829..462425
/gene="TMA22"
/locus_tag="YJR014W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0000184
nuclear-transcribed mRNA catabolic process,
nonsense-mediated decay [PMID:38198768]"
/experiment="EXISTENCE:mutant phenotype:GO:0032790
ribosome disassembly [PMID:34016977]"
/note="Ribosome-associated protein; involved along with
heterodimeric partner Tma20p in recycling of 40S ribosomal
subunits; involved in nonsense-mediated mRNA decay (NMD);
contains a putative RNA binding domain; localizes to the
cytoplasm in HTP studies; similar to human GRAP and human
DRP1, which interacts with human Tma20p homolog MCT-1;
protein abundance increases in response to DNA replication
stress"
/codon_start=1
/product="Tma22p"
/protein_id="NP_012548.1"
/db_xref="GeneID:853471"
/db_xref="SGD:S000003775"
/translation="MLREVIYCGICSYPPEYCEFSGKLKRCKVWLSENHADLYAKLYG
TDDNTQEVEAVTNKLAESSIGEAREEKLEKDLLKIQKKQENREQRELAKKLSSKVIIK
REARTKRKFIVAISGLEVFDIDMKKLAKTFASRFATGCSVSKNAEKKEEVVIQGDVMD
EVETYIHSLLEEKGLKDVKVETIDAKKKKKPAAEGAAK"
gene <462721..>464253
/locus_tag="YJR015W"
/db_xref="GeneID:853472"
mRNA <462721..>464253
/locus_tag="YJR015W"
/product="uncharacterized protein"
/transcript_id="NM_001181673.3"
/db_xref="GeneID:853472"
CDS 462721..464253
/locus_tag="YJR015W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762|PMID:14562095]"
/note="hypothetical protein; localizes to endoplasmic
reticulum and cytoplasm; null mutant exhibits abnormal
lipid metabolic phenotype; predicted to encode a membrane
transporter based on phylogenetic analysis; not an
essential gene; YJR015W has a paralog, SNG1, that arose
from the whole genome duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_012549.3"
/db_xref="GeneID:853472"
/db_xref="SGD:S000003776"
/translation="MTSSLDDIEPTAYNNMEADEEYCRRNDIHDLSSVVGDAVSQGVP
DMDGQTTDSSKDPEPNSEDKKAFPPSSGSFFSPNLQGQRKKVLLKFVFTNCLLAIICF
TMFVLFWGALYDTSKYLHKVKLLVVIQEPPVVILDNNSSMVVPSISYALPTFINKIPC
DWDIYNSPTFQAKFDVNTPQQVNDKVVDLVYDEKYWFAINIKPNATETLFESLINDTA
PLFNSTLFNQVVYETGRDPTNLKSTILPVAQTIEEYYHTFYTLNYLPPLLTNITQVYR
YALTNNARYIAAAGKYNYEYYDHRPFTDRILLAPTQIGVVYCLLLTFFQFLLYGPLHV
EMAKVLRPANGLIYRIAMSWFTFFFASLFFCTTTAIFQVDFTKSFGRGGFVVYWMSTW
LFMLAAGGANENAVMLVITLGPQYLGFWILSFVILNIAPSFFPLALNNNVYRYGYMMP
VHNVIDIYRVIFFDVTRRKMGRNYGILVALIALNTALLPFVGKYASRKLKQKALVAAK
QS"
gene complement(<464451..>466208)
/gene="ILV3"
/locus_tag="YJR016C"
/db_xref="GeneID:853473"
mRNA complement(<464451..>466208)
/gene="ILV3"
/locus_tag="YJR016C"
/product="dihydroxy-acid dehydratase ILV3"
/transcript_id="NM_001181674.1"
/db_xref="GeneID:853473"
CDS complement(464451..466208)
/gene="ILV3"
/locus_tag="YJR016C"
/EC_number="4.2.1.9"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:mutant phenotype:GO:0004160
dihydroxy-acid dehydratase activity [PMID:14124940]"
/experiment="EXISTENCE:mutant phenotype:GO:0009082
branched-chain amino acid biosynthetic process
[PMID:14124940]"
/note="Dihydroxyacid dehydratase; putative 2Fe-2S protein;
catalyzes third step in the common pathway leading to
biosynthesis of branched-chain amino acids"
/codon_start=1
/product="dihydroxy-acid dehydratase ILV3"
/protein_id="NP_012550.1"
/db_xref="GeneID:853473"
/db_xref="SGD:S000003777"
/translation="MGLLTKVATSRQFSTTRCVAKKLNKYSYIITEPKGQGASQAMLY
ATGFKKEDFKKPQVGVGSCWWSGNPCNMHLLDLNNRCSQSIEKAGLKAMQFNTIGVSD
GISMGTKGMRYSLQSREIIADSFETIMMAQHYDANIAIPSCDKNMPGVMMAMGRHNRP
SIMVYGGTILPGHPTCGSSKISKNIDIVSAFQSYGEYISKQFTEEEREDVVEHACPGP
GSCGGMYTANTMASAAEVLGLTIPNSSSFPAVSKEKLAECDNIGEYIKKTMELGILPR
DILTKEAFENAITYVVATGGSTNAVLHLVAVAHSAGVKLSPDDFQRISDTTPLIGDFK
PSGKYVMADLINVGGTQSVIKYLYENNMLHGNTMTVTGDTLAERAKKAPSLPEGQEII
KPLSHPIKANGHLQILYGSLAPGGAVGKITGKEGTYFKGRARVFEEEGAFIEALERGE
IKKGEKTVVVIRYEGPRGAPGMPEMLKPSSALMGYGLGKDVALLTDGRFSGGSHGFLI
GHIVPEAAEGGPIGLVRDGDEIIIDADNNKIDLLVSDKEMAQRKQSWVAPPPRYTRGT
LSKYAKLVSNASNGCVLDA"
gene complement(<466521..>467033)
/gene="ESS1"
/locus_tag="YJR017C"
/gene_synonym="PIN1; PTF1"
/db_xref="GeneID:853475"
mRNA complement(<466521..>467033)
/gene="ESS1"
/locus_tag="YJR017C"
/gene_synonym="PIN1; PTF1"
/product="peptidylprolyl isomerase ESS1"
/transcript_id="NM_001181675.1"
/db_xref="GeneID:853475"
CDS complement(466521..467033)
/gene="ESS1"
/locus_tag="YJR017C"
/gene_synonym="PIN1; PTF1"
/EC_number="5.2.1.8"
/experiment="EXISTENCE:curator inference:GO:0005634
nucleus [PMID:14704159|PMID:10899126|PMID:10531363]"
/experiment="EXISTENCE:direct assay:GO:0003755
peptidyl-prolyl cis-trans isomerase activity
[PMID:9867817|PMID:15728580]"
/experiment="EXISTENCE:genetic interaction:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:14704159]"
/experiment="EXISTENCE:genetic interaction:GO:0006369
termination of RNA polymerase II transcription
[PMID:19854134]"
/experiment="EXISTENCE:genetic interaction:GO:0045899
positive regulation of RNA polymerase II transcription
preinitiation complex assembly [PMID:15166139]"
/experiment="EXISTENCE:genetic interaction:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:10899126]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:14704159]"
/experiment="EXISTENCE:mutant phenotype:GO:0003755
peptidyl-prolyl cis-trans isomerase activity
[PMID:15728580]"
/experiment="EXISTENCE:mutant phenotype:GO:0006369
termination of RNA polymerase II transcription
[PMID:14704159|PMID:19854134|PMID:22778132]"
/experiment="EXISTENCE:mutant phenotype:GO:2000059
negative regulation of ubiquitin-dependent protein
catabolic process [PMID:19597489]"
/experiment="EXISTENCE:mutant phenotype:GO:2000749
positive regulation of rDNA heterochromatin formation
[PMID:10899127]"
/experiment="EXISTENCE:physical interaction:GO:0000993 RNA
polymerase II complex binding
[PMID:10899126|PMID:10531363]"
/experiment="EXISTENCE:physical interaction:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:10899126]"
/note="Peptidylprolyl-cis/trans-isomerase (PPIase);
specific for phosphorylated S/T residues N-terminal to
proline; regulates phosphorylation of RNAPII large subunit
(Rpo21p) C-terminal domain (CTD) at Ser7; associates with
phospho-Ser5 form of RNAPII in vivo; present along entire
coding length of genes; represses initiation of CUTs;
required for efficient termination of mRNA transcription,
trimethylation of histone H3; human ortholog PIN1 can
complement yeast null and ts mutants"
/codon_start=1
/product="peptidylprolyl isomerase ESS1"
/protein_id="NP_012551.2"
/db_xref="GeneID:853475"
/db_xref="SGD:S000003778"
/translation="MPSDVASRTGLPTPWTVRYSKSKKREYFFNPETKHSQWEEPEGT
NKDQLHKHLRDHPVRVRCLHILIKHKDSRRPASHRSENITISKQDATDELKTLITRLD
DDSKTNSFEALAKERSDCSSYKRGGDLGWFGRGEMQPSFEDAAFQLKVGEVSDIVESG
SGVHVIKRVG"
gene complement(<467232..>468281)
/gene="TES1"
/locus_tag="YJR019C"
/gene_synonym="PTE1"
/db_xref="GeneID:853477"
mRNA complement(<467232..>468281)
/gene="TES1"
/locus_tag="YJR019C"
/gene_synonym="PTE1"
/product="palmitoyl-CoA hydrolase"
/transcript_id="NM_001181677.3"
/db_xref="GeneID:853477"
CDS complement(467232..468281)
/gene="TES1"
/locus_tag="YJR019C"
/gene_synonym="PTE1"
/EC_number="3.1.2.2"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:10092594]"
/experiment="EXISTENCE:direct assay:GO:0008474
palmitoyl-(protein) hydrolase activity [PMID:10092594]"
/experiment="EXISTENCE:direct assay:GO:0047617 fatty
acyl-CoA hydrolase activity [PMID:10092594]"
/experiment="EXISTENCE:direct assay:GO:0052816 long-chain
fatty acyl-CoA hydrolase activity [PMID:10092594]"
/experiment="EXISTENCE:mutant phenotype:GO:0006635 fatty
acid beta-oxidation [PMID:19830908]"
/experiment="EXISTENCE:mutant phenotype:GO:0019395 fatty
acid oxidation [PMID:10092594]"
/note="Peroxisomal acyl-CoA thioesterase; likely to be
involved in fatty acid oxidation rather than fatty acid
synthesis; conserved protein also found in human
peroxisomes; TES1 mRNA levels increase during growth on
fatty acids"
/codon_start=1
/product="palmitoyl-CoA hydrolase"
/protein_id="NP_012553.3"
/db_xref="GeneID:853477"
/db_xref="SGD:S000003780"
/translation="MSASKMAMSNLEKILELVPLSPTSFVTKYLPAAPVGSKGTFGGT
LVSQSLLASLHTVPLNFFPTSLHSYFIKGGDPRTKITYHVQNLRNGRNFIHKQVSAYQ
HDKLIFTSMILFAVQRSKEHDSLQHWETIPGLQGKQPDPHRYEEATSLFQKEVLDPQK
LSRYASLSDRFQDATSMSKYVDAFQYGVMEYQFPKDMFYSARHTDELDYFVKVRPPIT
TVEHAGDESSLHKHHPYRIPKSITPENDARYNYVAFAYLSDSYLLLTIPYFHNLPLYC
HSFSVSLDHTIYFHQLPHVNNWIYLKISNPRSHWDKHLVQGKYFDTQSGRIMASVSQE
GYVVYGSERDIRAKF"
gene complement(<468555..>469579)
/gene="REC107"
/locus_tag="YJR021C"
/gene_synonym="MER2"
/db_xref="GeneID:853478"
mRNA complement(join(<468555..469183,469264..>469579))
/gene="REC107"
/locus_tag="YJR021C"
/gene_synonym="MER2"
/product="Rec107p"
/transcript_id="NM_001181679.1"
/db_xref="GeneID:853478"
CDS complement(join(468555..469183,469264..469579))
/gene="REC107"
/locus_tag="YJR021C"
/gene_synonym="MER2"
/experiment="EXISTENCE:direct assay:GO:0000794 condensed
nuclear chromosome [PMID:16783010]"
/experiment="EXISTENCE:mutant phenotype:GO:0007131
reciprocal meiotic recombination [PMID:8536989]"
/note="Protein involved in early stages of meiotic
recombination; involved in coordination between the
initiation of recombination and the first division of
meiosis; part of a complex (Rec107p-Mei4p-Rec114p)
required for ds break formation"
/codon_start=1
/product="Rec107p"
/protein_id="NP_012555.1"
/db_xref="GeneID:853478"
/db_xref="SGD:S000003782"
/translation="MVARGRTDEISTDVSEANSEHSLMITETSSPFRSIFSHSGKVAN
AGALEESDKQILEWAGKLELESMELRENSDKLIKVLNENSKTLCKSLNKFNQLLEQDA
ATNGNVKTLIKDLASQIENQLDKVSTAMLSKGDEKKTKSDSSYRQVLVEEISRYNSKI
TRHVTNKQHETEKSMRCTQEMLFNVGSQLEDVHKVLLSLSKDMHSLQTRQTALEMAFR
EKADHAYDRPDVSLNGTTLLHDMDEAHDKQRKKSVPPPRMMVTRSMKRRRSSSPTLST
SQNHNSEDNDDASHRLKRAARTIIPWEELRPDTLESEL"
gene <469784..>470113
/gene="LSM8"
/locus_tag="YJR022W"
/db_xref="GeneID:853479"
mRNA <469784..>470113
/gene="LSM8"
/locus_tag="YJR022W"
/product="U4/U6-U5 snRNP complex subunit LSM8"
/transcript_id="NM_001181680.1"
/db_xref="GeneID:853479"
CDS 469784..470113
/gene="LSM8"
/locus_tag="YJR022W"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:15075370|PMID:10369684]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12730603]"
/experiment="EXISTENCE:direct assay:GO:0005688 U6 snRNP
[PMID:11720284|PMID:10369684]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:18029398]"
/experiment="EXISTENCE:direct assay:GO:0046540 U4/U6 x U5
tri-snRNP complex [PMID:10449419|PMID:10377396]"
/experiment="EXISTENCE:mutant phenotype:GO:0000398 mRNA
splicing, via spliceosome [PMID:10428970]"
/note="Lsm (Like Sm) protein; forms heteroheptameric
complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and
Lsm7p) that is part of spliceosomal U6 snRNP and is also
implicated in processing of pre-tRNA, pre-snoRNA, and
pre-rRNA"
/codon_start=1
/product="U4/U6-U5 snRNP complex subunit LSM8"
/protein_id="NP_012556.2"
/db_xref="GeneID:853479"
/db_xref="SGD:S000003783"
/translation="MSATLKDYLNKRVVIIKVDGECLIASLNGFDKNTNLFITNVFNR
ISKEFICKAQLLRGSEIALVGLIDAENDDSLAPIDEKKVPMLKDTKNKIENEHVIWEK
VYESKTK"
gene complement(<470230..>470964)
/gene="MDE1"
/locus_tag="YJR024C"
/db_xref="GeneID:853481"
mRNA complement(<470230..>470964)
/gene="MDE1"
/locus_tag="YJR024C"
/product="methylthioribulose 1-phosphate dehydratase MDE1"
/transcript_id="NM_001181682.3"
/db_xref="GeneID:853481"
CDS complement(470230..470964)
/gene="MDE1"
/locus_tag="YJR024C"
/EC_number="4.2.1.109"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0019509
L-methionine salvage from methylthioadenosine
[PMID:18625006]"
/experiment="EXISTENCE:mutant phenotype:GO:0046570
methylthioribulose 1-phosphate dehydratase activity
[PMID:18625006]"
/note="5'-methylthioribulose-1-phosphate dehydratase; acts
in the methionine salvage pathway; potential Smt3p
sumoylation substrate; expression downregulated by
caspofungin and deletion mutant is caspofungin resistant"
/codon_start=1
/product="methylthioribulose 1-phosphate dehydratase MDE1"
/protein_id="NP_012558.3"
/db_xref="GeneID:853481"
/db_xref="SGD:S000003785"
/translation="MSSQDVLIHSDDPCHPANLICTLCKQFFHNNWCTGTGGGISIKD
PNTNYYYLAPSGVQKEKMIPEDLFVMDAQTLEYLRSPKLYKPSACTPLFLACYQKKNA
GAIIHTHSQNAVICSLLFGDEFRIANIEQIKAIPSGKVDPVTKKPMALSFFDTLKIPI
IENMAHEDELIDDLHKTFKDYPDTCAVIVRRHGIFVWGPTIDKAKIFNEAIDYLMELA
IKMYQMGIPPDCGIGEEKKHLKMASP"
gene complement(<471138..>471671)
/gene="BNA1"
/locus_tag="YJR025C"
/gene_synonym="HAD1"
/db_xref="GeneID:853482"
mRNA complement(<471138..>471671)
/gene="BNA1"
/locus_tag="YJR025C"
/gene_synonym="HAD1"
/product="3-hydroxyanthranilate 3,4-dioxygenase"
/transcript_id="NM_001181683.1"
/db_xref="GeneID:853482"
CDS complement(471138..471671)
/gene="BNA1"
/locus_tag="YJR025C"
/gene_synonym="HAD1"
/EC_number="1.13.11.6"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11901108]"
/experiment="EXISTENCE:genetic interaction:GO:0034354 'de
novo' NAD+ biosynthetic process from L-tryptophan
[PMID:12062417]"
/experiment="EXISTENCE:mutant phenotype:GO:0000334
3-hydroxyanthranilate 3,4-dioxygenase activity
[PMID:9539135]"
/note="3-hydroxyanthranilic acid dioxygenase; required for
the de novo biosynthesis of NAD from tryptophan via
kynurenine; expression regulated by Hst1p"
/codon_start=1
/product="3-hydroxyanthranilate 3,4-dioxygenase"
/protein_id="NP_012559.1"
/db_xref="GeneID:853482"
/db_xref="SGD:S000003786"
/translation="MFNTTPINIDKWLKENEGLLKPPVNNYCLHKGGFTVMIVGGPNE
RTGYHINPTPEWFYQKKGSMLLKVVDETDAEPKFIDIIINEGDSYLLPGNVPHSPVRF
ADTVGIVVEQDRPGGENDKIRWYCSHCRQVVHESELQMLDLGTQVKEAILDFENDVEK
RTCFHCKTLNYARPQSN"
mobile_element 472463..478384
/note="YJRWTy1-1; Ty1 element, LTR retrotransposon of the
Copia (Pseudoviridae) group; contains co-transcribed genes
TYA Gag and TYB Pol, encoding proteins involved in
structure and function of virus-like particles, flanked by
two direct repeats"
/mobile_element_type="retrotransposon:YJRWTy1-1"
/db_xref="SGD:S000007048"
repeat_region 472463..472800
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007040"
gene <472760..>478028
/locus_tag="YJR027W"
/db_xref="GeneID:853484"
mRNA <472760..>478028
/locus_tag="YJR027W"
/product="gag-pol fusion protein"
/transcript_id="NM_001181685.2"
/db_xref="GeneID:853484"
CDS join(472760..474061,474063..478028)
/locus_tag="YJR027W"
/EC_number="2.7.7.7"
/EC_number="2.7.7.49"
/EC_number="3.1.26.4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9448009]"
/ribosomal_slippage
/note="Retrotransposon TYA Gag and TYB Pol genes;
transcribed/translated as one unit; polyprotein is
processed to make a nucleocapsid-like protein (Gag),
reverse transcriptase (RT), protease (PR), and integrase
(IN); similar to retroviral genes"
/codon_start=1
/product="gag-pol fusion protein"
/protein_id="NP_012560.1"
/db_xref="GeneID:853484"
/db_xref="SGD:S000003788"
/translation="MESQQLSQHSHISHGSACASVTSKEVHTNQDPLDVSASKTEECE
KASTKANSQQTTTPASSAVPENPHHASPQPASVPPPQNGPYPQQCMMTQNQANPSGWS
FYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVRQITDDELT
FLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQ
YNGSTPADAFETKVTNIIDRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNM
TVAELFLDIHAIYEEQQGSRNSKPNYRRNLSDEKNDSRSYTNTTKPKVIARNPQKTNN
SKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLGQELTESTVNHTNHS
DDELPGHLLLDSGASRTLIRSAHHIHSASSNPDINVVDAQKRNIPINAIGDLQFHFQD
NTKTSIKVLHTPNIAYDLLSLNELAAVDITACFTKNVLERSDGTVLAPIVQYGDFYWV
SKRYLLPSNISVPTINNVHTSESTRKYPYPFIHRMLAHANAQTIRYSLKNNTITYFNE
SDVDWSSAIDYQCPDCLIGKSTKHRHIKGSRLKYQNSYEPFQYLHTDIFGPVHNLPKS
APSYFISFTDETTKFRWVYPLHDRREDSILDVFTTILAFIKNQFQASVLVIQMDRGSE
YTNRTLHKFLEKNGITPCYTTTADSRAHGVAERLNRTLLDDCRTQLQCSGLPNHLWFS
AIEFSTIVRNSLASPKSKKSARQHAGLAGLDISTLLPFGQPVIVNDHNPNSKIHPRGI
PGYALHPSRNSYGYIIYLPSLKKTVDTTNYVILQGKESRLDQFNYDALTFDEDLNRLT
ASYQSFIASNEIQESNDLNIESDHDFQSDIELHPEQPRNVLSKAVSPTDSTPPSTHTE
DSKRVSKTNIRAPREVDPNISESNILPSKKRSSTPQISNIESTGSGGMHKLNVPLLAP
MSQSNTHESSHASKSKDFRHSDSYSENETNHTNVPISSTGGTNNKTVPQISDQETEKR
IIHRSPSIDASPPENNSSHNIVPIKTPTTVSEQNTEESIIADLPLPDLPPESPTEFPD
PFKELPPINSHQTNSSLGGIGDSNAYTTINSKKRSLEDNETEIKVSRDTWNTKNMRSL
EPPRSKKRIHLIAAVKAVKSIKPIRTTLRYDEAITYNKDIKEKEKYIEAYHKEVNQLL
KMKTWDTDEYYDRKEIDPKRVINSMFIFNKKRDGTHKARFVARGDIQHPDTYDTGMQS
NTVHHYALMTSLSLALDNNYYITQLDISSAYLYADIKEELYIRPPPHLGMNDKLIRLK
KSHYGLKQSGANWYETIKSYLIKQCGMEEVRGWSCVFKNSQVTICLFVDDMILFSKDL
NANKKIITTLKKQYDTKIINLGESDNEIQYDILGLEIKYQRGKYMKLGMENSLTEKIP
KLNVPLNPKGRKLSAPGQPGLYIDQDELEIDEDEYKEKVHEMQKLIGLASYVGYKFRF
DLLYYINTLAQHILFPSRQVLDMTYELIQFMWDTRDKQLIWHKNKPTEPDNKLVAISD
ASYGNQPYYKSQIGNIFLLNGKVIGGKSTKASLTCTSTTEAEIHAISESVPLLNNLSY
LIQELNKKPIIKGLLTDSRSTISIIKSTNEEKFRNRFFGTKAMRLRDEVSGNNLYVYY
IETKKNIADVMTKPLPIKTFKLLTNKWIH"
gene <472760..>474082
/locus_tag="YJR026W"
/db_xref="GeneID:853483"
mRNA <472760..>474082
/locus_tag="YJR026W"
/product="gag protein"
/transcript_id="NM_001181684.3"
/db_xref="GeneID:853483"
CDS 472760..474082
/locus_tag="YJR026W"
/note="Retrotransposon TYA Gag gene co-transcribed with
TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is
a nucleocapsid protein that is the structural constituent
of virus-like particles (VLPs); similar to retroviral Gag"
/codon_start=1
/product="gag protein"
/protein_id="NP_012561.3"
/db_xref="GeneID:853483"
/db_xref="SGD:S000003787"
/translation="MESQQLSQHSHISHGSACASVTSKEVHTNQDPLDVSASKTEECE
KASTKANSQQTTTPASSAVPENPHHASPQPASVPPPQNGPYPQQCMMTQNQANPSGWS
FYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVRQITDDELT
FLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQ
YNGSTPADAFETKVTNIIDRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNM
TVAELFLDIHAIYEEQQGSRNSKPNYRRNLSDEKNDSRSYTNTTKPKVIARNPQKTNN
SKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLRPGTY"
mobile_element 478051..483972
/note="YJRWTy1-2; Ty1 element, LTR retrotransposon of the
Copia (Pseudoviridae) group; contains co-transcribed genes
TYA Gag and TYB Pol, encoding proteins involved in
structure and function of virus-like particles, flanked by
two direct repeats"
/mobile_element_type="retrotransposon:YJRWTy1-2"
/db_xref="SGD:S000007049"
repeat_region 478051..478384
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007041"
gene <478344..>483612
/locus_tag="YJR029W"
/db_xref="GeneID:853486"
mRNA <478344..>483612
/locus_tag="YJR029W"
/product="gag-pol fusion protein"
/transcript_id="NM_001181687.2"
/db_xref="GeneID:853486"
CDS join(478344..479644,479646..483612)
/locus_tag="YJR029W"
/EC_number="2.7.7.7"
/EC_number="2.7.7.49"
/EC_number="3.1.26.4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9448009]"
/ribosomal_slippage
/note="Retrotransposon TYA Gag and TYB Pol genes;
transcribed/translated as one unit; polyprotein is
processed to make a nucleocapsid-like protein (Gag),
reverse transcriptase (RT), protease (PR), and integrase
(IN); similar to retroviral genes"
/codon_start=1
/product="gag-pol fusion protein"
/protein_id="NP_012562.1"
/db_xref="GeneID:853486"
/db_xref="SGD:S000003790"
/translation="MESQQLSQHSHISHGSACASVTSKEVHTNQDPLDVSASKTEECE
KASTKANSQQTTTPASSAVPENPHHASPQTAQSHSPQNGPYPQQCMMTQNQANPSGWS
FYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVRQITDDELT
FLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQ
YNGSTPADAFETKVTNIIDRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNM
TVAELFLDIHAIYEEQQGSRNSKPNYRRNPSDEKNDSRSYTNTTKPKVIARNPQKTNN
SKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLGQKLTESTVNHTNHS
DDELPGHLLLDSGASRTLIRSAHHIHSASSNPDINVVDAQKRNIPINAIGDLQFHFQD
NTKTSIKVLHTPNIAYDLLSLNELAAVDITACFTKNVLERSDGTVLAPIVKYGDFYWV
SKKYLLPSNISVPTINNVHTSESTRKYPYPFIHRMLAHANAQTIRYSLKNNTITYFNE
SDVDWSSAIDYQCPDCLIGKSTKHRHIKGSRLKYQNSYEPFQYLHTDIFGPVHNLPKS
APSYFISFTDETTKFRWVYPLHDRREDSILDVFTTILAFIKNQFQASVLVIQMDRGSE
YTNRTLHKFLEKNGITPCYTTTADSRAHGVAERLNRTLLDDCRTQLQCSGLPNHLWFS
AIEFSTIVRNSLASPKSKKSARQHAGLAGLDISTLLPFGQPVIVNDHNPNSKIHPRGI
PGYALHPSRNSYGYIIYLPSLKKTVDTTNYVILQGKESRLDQFNYDALTFDEDLNRLT
ASYQSFIASNEIQQSDDLNIESDHDFQSDIELHPEQPRNVLSKAVSPTDSTPPSTHTE
DSKRVSKTNIRAPREVDPNISESNILPSKKRSSTPQISNIESTGSGGMHKLNVPLLAP
MSQSNTHESSHASKSKDFRHSDSYSENETNHTNVPISSTGGTNNKTVPQISDQETEKR
IIHRSPSIDASPPENNSSHNIVPIKTPTTVSEQNTEESIIADLPLPDLPPESPTEFPD
PFKELPPINSRQTNSSLGGIGDSNAYTTINSKKRSLEDNETEIKVSRDTWNTKNMRSL
EPPRSKKRIHLIAAVKAVKSIKPIRTTLRYDEAITYNKDIKEKEKYIEAYHKEVNQLL
KMKTWDTDEYYDRKEIDPKRVINSMFIFNKKRDGTHKARFVARGDIQHPDTYDSGMQS
NTVHHYALMTSLSLALDNNYYITQLDISSAYLYADIKEELYIRPPPHLGMNDKLIRLK
KSLYGLKQSGANWYETIKSYLIQQCGMEEVRGWSCVFKNSQVTICLFVDDMVLFSKNL
NSNKRIIEKLKMQYDTKIINLGESDEEIQYDILGLEIKYQRGKYMKLGMENSLTEKIP
KLNVPLNPKGRKLSAPGQPGLYIDQQELELEEDDYKMKVHEMQKLIGLASYVGYKFRF
DLLYYINTLAQHILFPSKQVLDMTYELIQFIWNTRDKQLIWHKSKPVKPTNKLVVISD
ASYGNQPYYKSQIGNIYLLNGKVIGGKSTKASLTCTSTTEAEIHAISESVPLLNNLSY
LIQELDKKPITKGLLTDSKSTISIIISNNEEKFRNRFFGTKAMRLRDEVSGNHLHVCY
IETKKNIADVMTKPLPIKTFKLLTNKWIH"
gene <478344..>479666
/locus_tag="YJR028W"
/db_xref="GeneID:853485"
mRNA <478344..>479666
/locus_tag="YJR028W"
/product="gag protein"
/transcript_id="NM_001181686.1"
/db_xref="GeneID:853485"
CDS 478344..479666
/locus_tag="YJR028W"
/note="Retrotransposon TYA Gag gene co-transcribed with
TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is
a nucleocapsid protein that is the structural constituent
of virus-like particles (VLPs); similar to retroviral Gag"
/codon_start=1
/product="gag protein"
/protein_id="NP_012563.1"
/db_xref="GeneID:853485"
/db_xref="SGD:S000003789"
/translation="MESQQLSQHSHISHGSACASVTSKEVHTNQDPLDVSASKTEECE
KASTKANSQQTTTPASSAVPENPHHASPQTAQSHSPQNGPYPQQCMMTQNQANPSGWS
FYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVRQITDDELT
FLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQ
YNGSTPADAFETKVTNIIDRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNM
TVAELFLDIHAIYEEQQGSRNSKPNYRRNPSDEKNDSRSYTNTTKPKVIARNPQKTNN
SKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLRPETY"
repeat_region 483635..483972
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007042"
gene complement(<483959..>486196)
/gene="RBH2"
/locus_tag="YJR030C"
/db_xref="GeneID:853487"
mRNA complement(<483959..>486196)
/gene="RBH2"
/locus_tag="YJR030C"
/product="Rbh2p"
/transcript_id="NM_001181688.3"
/db_xref="GeneID:853487"
CDS complement(483959..486196)
/gene="RBH2"
/locus_tag="YJR030C"
/experiment="EXISTENCE:mutant phenotype:GO:0019320 hexose
catabolic process [PMID:23185390]"
/note="hypothetical protein; expression repressed in
carbon limited vs carbon replete chemostat cultures;
non-essential gene; contains a PH-like domain; RBH2 has a
paralog, RBH1, that arose from the whole genome
duplication"
/codon_start=1
/product="Rbh2p"
/protein_id="NP_012564.3"
/db_xref="GeneID:853487"
/db_xref="SGD:S000003791"
/translation="MELFNKEEASFETLLKRLLVVCESHSRYHGSSLDPMVKVGHEMR
KISGYLRCILRKHAANHDDMSLTQSIVNSYKSLFKDAQILDLYHNLLFGCMHLLLDAN
MSYFRMDSQKLFAVLLFKVYYKLRDIFYVTNEVRLGSLISAFVYKFKSCYDFISCNSL
KYGSVRDVISGEVSLINLPPIDSNKVINRAYYRLDVKKLAINNKLVEILELDNGEIAI
FEVLSEKMPYTLQTIDNLFQSLALGNHDLMNVGRSLLFRPFRSGDLDLIRLDDSGAKL
KVPINNSIVLRLTCKDPIQWQEYWKHVIRKLFDSTATKEYKRSGSKISQQVYVRSNNP
DYTSPKRNDDMPISSVKISDTIHNGRTLHRSIPLPGSLSSLIETSNEYPDEESLSIMS
ERATVSEDSDLDTSLKDIESLSCEKLIELDKSIQVPLSPKYMDTPTLKNIRTASQTFS
LESVSPELIESVASEIDDSESIISEDGKDKRDKDLFDPDIDFYKPTLYRRKSSSLLSI
FSKNKKNLTIDIPKNHSRSLFSLPGNQQSVTPVSATPHDDNVDETYVSFPLSINTSGG
AVYFENDSVKVSLWNGKSWVPLSKDMLCLSLILSGDNETLLIVYKDFEKEKCKLVVKL
EPTWKYNRSTAQDVQLRIPSSDFKASVFGTLHDLTLSIRCAQAAKLVNVLQYQLQSSQ
TSSLSPSTTTGTLSTVSSSSCFSRNVTRSSTENSELANMKDSSEYISSSLLLSSVKVR
QLRNM"
gene complement(<486586..>490812)
/gene="GEA1"
/locus_tag="YJR031C"
/db_xref="GeneID:853488"
mRNA complement(<486586..>490812)
/gene="GEA1"
/locus_tag="YJR031C"
/product="Arf family guanine nucleotide exchange factor
GEA1"
/transcript_id="NM_001181689.1"
/db_xref="GeneID:853488"
CDS complement(486586..490812)
/gene="GEA1"
/locus_tag="YJR031C"
/experiment="EXISTENCE:direct assay:GO:0000137 Golgi cis
cisterna [PMID:11294905]"
/experiment="EXISTENCE:direct assay:GO:0005085
guanyl-nucleotide exchange factor activity
[PMID:10430582]"
/experiment="EXISTENCE:direct assay:GO:0005933 cellular
bud [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0006890
retrograde vesicle-mediated transport, Golgi to
endoplasmic reticulum [PMID:11294905]"
/experiment="EXISTENCE:genetic interaction:GO:0030036
actin cytoskeleton organization [PMID:14668359]"
/experiment="EXISTENCE:mutant phenotype:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:8945477|PMID:11493664]"
/experiment="EXISTENCE:mutant phenotype:GO:0006891
intra-Golgi vesicle-mediated transport [PMID:11493664]"
/experiment="EXISTENCE:mutant phenotype:GO:0016236
macroautophagy [PMID:20444982]"
/note="Guanine nucleotide exchange factor for ADP
ribosylation factors (ARFs); involved in vesicular
transport between the Golgi and ER, Golgi organization,
and actin cytoskeleton organization; GEA1 has a paralog,
GEA2, that arose from the whole genome duplication"
/codon_start=1
/product="Arf family guanine nucleotide exchange factor
GEA1"
/protein_id="NP_012565.1"
/db_xref="GeneID:853488"
/db_xref="SGD:S000003792"
/translation="MHDVPMETVLAVNPATMIVKECINLCSAMNKQSRDKSQTSVAAL
LGGGSDIFLSQSDSFVDSFHNLPTSSYHDPLISGLVQLRLKINDLKGLDSLNALELLK
PFLEIVSASSVSGYTTSLALDSLQKVFTLKIINKTFNDIQIAVRETVVALTHCRFEAS
KQISDDSVLLKVVTLLRDIITSSFGDYLSDTIIYDVLQTTLSLACNTQRSEVLRKTAE
VTIAGITVKLFTKLKLLDPPTKTEKYINDESYTDNNLKDDIIGTTTSDNDLSSTDDDS
AVADDNKNEKPVQQVIREQENDEETAEKAENVEPNYGITVIKDYLGLLLSLVMPENRM
KHTTSAMKLSLQLINAAIEISGDKFPLYPRLFSLISDPIFKSVLFIIQSSTQYSLLQA
TLQLFTSLVVILGDYLPMQIELTLRRIFEILEDTTISGDVSKQKPPAIRELIIEQLSI
LWIHSPAFFLQLFVNFDCNLDRSDLSIDFIKELTKFSLPAAAVNTSNNIPPICLEGVL
SLIENIYNDLQRFDRAEFVKNQKEIDILKQRDRKTEFILCVETFNEKAKKGIQMLIEK
GFIDSDSNRDIASFLFLNNGRLNKKTIGLLLCDPKKTSLLKEFIDLFDFKGLRVDEAI
RILLTKFRLPGESQQIERIVEAFSSKYSADQSNDKVELEDKKAGKNGSESMTEDDIIH
VQPDADSVFVLSYSIIMLNTDSHNPQVKDHMTFDDYSNNLRGCYNGKDFPRWYLHKIY
TSIKVKEIVMPEEHHGNERWFEDAWNNLISSTSVMTEMQRDFTNPISKLAQIDILQYE
KAIFSNVRDIILKTLFKIFTVASSDQISLRILDAISKCTFINYYFSFDQSYNDTVLHL
GEMTTLAQSSAKAVELDVDSIPLVEIFVEDTGSKISVSNQSIRLGQNFKAQLCTVLYF
QIIKEISDPSIVSTRLWNQIVQLILKLFENLLMEPNLPFFTNFHSLLKLPELPLPDPD
ISIRKAKMSRSLLSTFASYLKGDEEPSEEDIDFSIKAFECVKASHPLSSVFENNQLVS
PKMIETLLSSLVIEKTSENSPYFEQELLFLLEISIILISEASYGQEFGALIADHMINI
SNLDGLSKEAIARLASYKMFLVSRFDNPRDILSDLIEHDFLVKNEIFNTKYYESEWGK
QVINDLFTHLNDVKYNERALKNVKFWNFLRILISAKDRQFAVYTFLEKYIQNGDIFVD
DGNFMNILSLLDEMSCAGAVGTKWEQNYENSVEDGCEAPESNPYRSIIDLSSRSINIT
ADLLSTVGRSNSALNKNEIIAAIQGLAHQCLNPCDELGMQALQALENILLSRASQLRT
EKVAVDNLLETGLLPIFELDEIQDVKMKRITSILSVLSKIFLGQLVEGVTSNETFLRV
LNVFNKYVDDPTVERQLQELIISKREIEKE"
gene <491081..>492262
/gene="CPR7"
/locus_tag="YJR032W"
/db_xref="GeneID:853489"
mRNA <491081..>492262
/gene="CPR7"
/locus_tag="YJR032W"
/product="peptidylprolyl isomerase CPR7"
/transcript_id="NM_001181690.1"
/db_xref="GeneID:853489"
CDS 491081..492262
/gene="CPR7"
/locus_tag="YJR032W"
/EC_number="5.2.1.8"
/experiment="EXISTENCE:direct assay:GO:0003755
peptidyl-prolyl cis-trans isomerase activity
[PMID:10942767]"
/experiment="EXISTENCE:direct assay:GO:0042026 protein
refolding [PMID:10942767]"
/experiment="EXISTENCE:direct assay:GO:0051082 unfolded
protein binding [PMID:10942767]"
/experiment="EXISTENCE:physical interaction:GO:0005829
cytosol [PMID:10942767]"
/note="Peptidyl-prolyl cis-trans isomerase (cyclophilin);
catalyzes the cis-trans isomerization of peptide bonds
N-terminal to proline residues; binds to Hsp82p and
contributes to chaperone activity; plays a role in
determining prion variants"
/codon_start=1
/product="peptidylprolyl isomerase CPR7"
/protein_id="NP_012566.1"
/db_xref="GeneID:853489"
/db_xref="SGD:S000003793"
/translation="MIQDPLVYLDISIDKKPIGRIVCKLFREKAPKTTENFYKLCAGD
VKSPLKDQQYLSYKGNGFHRVVKNFMIQAGDIVFGTQKDSSSSSVGKGGCSIYADKEE
VKTDDESFCYGNFEDENLGEFVEPFTLGMANLGSPNTNNSQFFITTYAAPHLNGKHSI
FGQVVHGKSVVRTIENCRVDSDGVPESDVRISDCGVWEKTMGVPLYNASNDQIGGDVY
EEYPDDDTHFGDDDFGKALEAANIIKESGTLLFKKKDYSNAFFKYRKSLNYINEYMPE
PDVDKERNIQFINLKMKIYLNLSLVLFNLERYDDAIMYATYLLEMDNVPNRDQAKAYY
RRGNSYLKKKRLDEALQDYIFCKEKNPDDEVIEQRIEYVNRLIEENKEKTRKNISKFF
S"
gene complement(<492378..>496451)
/gene="RAV1"
/locus_tag="YJR033C"
/gene_synonym="SOI3"
/db_xref="GeneID:853490"
mRNA complement(<492378..>496451)
/gene="RAV1"
/locus_tag="YJR033C"
/gene_synonym="SOI3"
/product="Rav1p"
/transcript_id="NM_001181691.3"
/db_xref="GeneID:853490"
CDS complement(492378..496451)
/gene="RAV1"
/locus_tag="YJR033C"
/gene_synonym="SOI3"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11283612]"
/experiment="EXISTENCE:genetic interaction:GO:0007035
vacuolar acidification [PMID:11283612|PMID:11844802]"
/experiment="EXISTENCE:genetic interaction:GO:0043254
regulation of protein-containing complex assembly
[PMID:11844802]"
/experiment="EXISTENCE:mutant phenotype:GO:0007035
vacuolar acidification [PMID:11283612|PMID:11844802]"
/experiment="EXISTENCE:mutant phenotype:GO:0043254
regulation of protein-containing complex assembly
[PMID:11844802|PMID:11283612]"
/experiment="EXISTENCE:mutant phenotype:GO:0045022 early
endosome to late endosome transport [PMID:15090613]"
/experiment="EXISTENCE:physical interaction:GO:0043254
regulation of protein-containing complex assembly
[PMID:11283612]"
/experiment="EXISTENCE:physical interaction:GO:0043291
RAVE complex [PMID:11283612|PMID:11844802]"
/note="Subunit of RAVE complex (Rav1p, Rav2p, Skp1p); the
RAVE complex promotes assembly of the V-ATPase holoenzyme;
required for transport between the early and late
endosome/PVC and for localization of TGN membrane
proteins; potential Cdc28p substrate"
/codon_start=1
/product="Rav1p"
/protein_id="NP_012567.3"
/db_xref="GeneID:853490"
/db_xref="SGD:S000003794"
/translation="MSLNFLPGRPNATPQTACQATWQNHTIFAYCSGNNLIILTNKFT
RLQTIYTQSDCTAVDINSQNGFIALSFHNRVLIYKPIHQIMQNPKWTQCCQLFHDDTP
VNCLRWSSDNELAIGSDFLSFWKIKDNFGVYQPILQWNQKQPKPVYNVIISQDSQLIV
SIGKYDCNAKLWKRVSIVGEQAIFNLTMLPHPKPITAMRWKKEPDQVSKNNTASHALY
TLCEDKVLRIWSCFEMEKNHTVQIWGEVPLSPTQKFCVIIDNWIIRQTLSVKDSEIFD
ISDSDIVILGSMTGEMEVLALNNLSQDPPKPMTKKTISHKKVKKATMLNDTRYLYLPE
IQPYDNVKGKLSFLVHDLQGVIRHLLIDILQLINNKTEDLSAALEHKFTGHNKSVQKL
VRSSDGEALLTTSRFSENGVWYPQKLNHGVSLRLQNTIQTESPIKFAVVHELGKQVIC
LLENGALQAWECPTNRKEDSEQKQSYLRVETRLKEEKKIHPIVMLNTPEPKHSHERHF
TALIFSDGSIKAFEVSLTRGIFEVKSDSLDIDGDDIYKISIIDPVHQTFVSNRPLISL
ITKKGLTRTYKAIVNYNDRHVQWIKACEINTGIMNCTCIRGSSTGKLCIVNSTGKVMS
LWDLNRGVLEYEETFHNPIEDIDWTSTEYGQSIVSIGFTGYALLYTQLRYDYTNNTPS
YLPIEKIDITAHTAHNIGDSVWMKNGTFVVASGNQFYIKDKSLDLTDPFTYQSIGSRK
ILSNDILHLSSVLNGPLPVYHPQFLIQAIYANKLQLVKELLLRLFLALRKLDFESQDV
SNLDSNLGMDPLKYFIAKDRDYPVESFPDPYPCFNKTVSLALTEQLTKTTLPYLTRHQ
QITLITVIEAVDEVTKNENIVDYNGVRFLLGVKLFLSHKNIQKSILMRDVSWALHSDN
KEILLSSIDRHITSWNRAREYRIAYWIKEQDLVKKFEDIAKYEFSKDDKRDPSRCAIF
YLALKKKQILLSLWKMAIGHPEQQKMVRFISNDFTVPRWRTAALKNAFVLLSKHRYMD
AAVFFLLTDSLKDCVNVLCKQVHDMDLAIGVCRVYEGDNGPVLGELLTAQMLPETIKE
NDRWKASFIYWKLRKQEVAIKALLTAPIDLENNSSIVDKEVCVNRSFLVEDPALLYLY
NHLRNRNLKYFIGSLNVEAKIECTLILRVTDILCRMGCNYLAVSLVKNWKFIERNSIP
VQKLLKSPTKDRAYSAIGAMASEPISTARMRPSLFDKFGSPSASDIESPNPKLPNSLL
DDFLQPPPNSTSSNSLAQSSSSAPRSILDEFVSPSYSQHKENLTPKAPNDSVGETDNS
ENRKDKLSKDILDDLSSQKPQKPKKSAITKNLLDDFV"
gene <496683..>497009
/gene="PET191"
/locus_tag="YJR034W"
/db_xref="GeneID:853491"
mRNA <496683..>497009
/gene="PET191"
/locus_tag="YJR034W"
/product="Pet191p"
/transcript_id="NM_001181692.1"
/db_xref="GeneID:853491"
CDS 496683..497009
/gene="PET191"
/locus_tag="YJR034W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:18503002]"
/experiment="EXISTENCE:direct assay:GO:0005758
mitochondrial intermembrane space [PMID:22984289]"
/experiment="EXISTENCE:mutant phenotype:GO:0033617
mitochondrial respiratory chain complex IV assembly
[PMID:8381337|PMID:18503002]"
/note="Protein required for assembly of cytochrome c
oxidase; exists as an oligomer; described as both an
integral mitochondrial inner membrane protein facing the
intermembrane space (IMS) and as a soluble IMS protein;
contains a twin Cx9C motif; imported into the IMS via the
MIA import machinery"
/codon_start=1
/product="Pet191p"
/protein_id="NP_012568.1"
/db_xref="GeneID:853491"
/db_xref="SGD:S000003795"
/translation="MVASCKDQKKAVAICLQRSPCVMIERHNPQECLDNPELNKDLPE
LCIAQMKAFLDCKRGIVDMTKRFTGNAPLSTGKYDQQYENLCKGKFDPREEMEKLKLL
NSQQKD"
gene <497355..>500612
/gene="RAD26"
/locus_tag="YJR035W"
/db_xref="GeneID:853492"
mRNA <497355..>500612
/gene="RAD26"
/locus_tag="YJR035W"
/product="DNA-dependent ATPase RAD26"
/transcript_id="NM_001181693.1"
/db_xref="GeneID:853492"
CDS 497355..500612
/gene="RAD26"
/locus_tag="YJR035W"
/EC_number="3.6.4.12"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008094
ATP-dependent activity, acting on DNA [PMID:8702468]"
/experiment="EXISTENCE:genetic interaction:GO:0006283
transcription-coupled nucleotide-excision repair
[PMID:17023424]"
/experiment="EXISTENCE:genetic interaction:GO:0006289
nucleotide-excision repair [PMID:17023424]"
/experiment="EXISTENCE:mutant phenotype:GO:0006283
transcription-coupled nucleotide-excision repair
[PMID:32690696|PMID:7957102]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:23991048]"
/experiment="EXISTENCE:mutant phenotype:GO:0006974 DNA
damage response [PMID:23991048]"
/note="Protein involved in transcription-coupled
nucleotide excision repair; repairs UV-induced DNA
lesions; recruitment to DNA lesions is dependent on an
elongating RNA polymerase II; homolog of human CSB
protein"
/codon_start=1
/product="DNA-dependent ATPase RAD26"
/protein_id="NP_012569.1"
/db_xref="GeneID:853492"
/db_xref="SGD:S000003796"
/translation="MEDKEQQDNAKLENNESLKDLGVNVLSQSSLEEKIANDVTNFSN
LQSLQQEETRLERSKTALQRYVNKKNHLTRKLNNTTRISVKQNLRDQIKNLQSDDIER
VLKDIDDIQSRIKELKEQVDQGAENKGSKEGLQRPGETEKEFLIRTGKITAFGHKAGF
SLDTANREYAKNDEQKDEDFEMATEQMVENLTDEDDNLSDQDYQMSGKESEDDEEEEN
DDKILKELEDLRFRGQPGEAKDDGDELYYQERLKKWVKQRSCGSQRSSDLPEWRRPHP
NIPDAKLNSQFKIPGEIYSLLFNYQKTCVQWLYELYQQNCGGIIGDEMGLGKTIQVIA
FIAALHHSGLLTGPVLIVCPATVMKQWCNEFQHWWPPLRTVILHSMGSGMASDQKFKM
DENDLENLIMNSKPSDFSYEDWKNSTRTKKALESSYHLDKLIDKVVTDGHILITTYVG
LRIHSDKLLKVKWQYAVLDEGHKIRNPDSEISLTCKKLKTHNRIILSGTPIQNNLTEL
WSLFDFIFPGKLGTLPVFQQQFVIPINIGGYANATNIQVQTGYKCAVALRDLISPYLL
RRVKADVAKDLPQKKEMVLFCKLTKYQRSKYLEFLHSSDLNQIQNGKRNVLFGIDILR
KICNHPDLLDRDTKRHNPDYGDPKRSGKMQVVKQLLLLWHKQGYKALLFTQSRQMLDI
LEEFISTKDPDLSHLNYLRMDGTTNIKGRQSLVDRFNNESFDVFLLTTRVGGLGVNLT
GANRIIIFDPDWNPSTDMQARERAWRIGQKREVSIYRLMVGGSIEEKIYHRQIFKQFL
TNRILTDPKQKRFFKIHELHDLFSLGGENGYSTEELNEEVQKHTENLKNSKSEESDDF
EQLVNLSGVSKLESFYNGKEKKENSKTEDDRLIEGLLGGESNLETVMSHDSVVNSHAG
SSSSNIITKEASRVAIEAVNALRKSRKKITKQYEIGTPTWTGRFGKAGKIRKRDPLKN
KLTGSAAILGNITKSQKEASKEARQENYDDGITFARSKEINSNTKTLENIRAYLQKQN
NFFSSSVSILNSIGVSLSDKEDVIKVRALLKTIAQFDKERKGWVLDEEFRNNNAS"
gene complement(<500713..>503391)
/gene="HUL4"
/locus_tag="YJR036C"
/db_xref="GeneID:853494"
mRNA complement(<500713..>503391)
/gene="HUL4"
/locus_tag="YJR036C"
/product="putative E3 ubiquitin-protein ligase HUL4"
/transcript_id="NM_001181694.3"
/db_xref="GeneID:853494"
CDS complement(500713..503391)
/gene="HUL4"
/locus_tag="YJR036C"
/EC_number="2.3.2.26"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0031499 TRAMP
complex [PMID:15828860]"
/note="Protein with similarity to hect domain E3
ubiquitin-protein ligases; not essential for viability;
found in association with Trf4 in TRAMP complex"
/codon_start=1
/product="putative E3 ubiquitin-protein ligase HUL4"
/protein_id="NP_012570.3"
/db_xref="GeneID:853494"
/db_xref="SGD:S000003797"
/translation="MVSLFDKLNAKKDGRDGSVSKELLSHSVAHTKNRLPKSGRRTSE
RSLAASVKDGSCSNSKSNKRNSSASVSGEEDKSCLISLNCLCCGVPLRFPASITKFRC
SACQVTVIVKEPEINSNLESSTHISCTLEGLQMVVRRCHDDLQRLKKTGILDKERKGL
IFQPVITYLLDRFHDVSILNRSFLVHDGGKNIKMLNYEVLQRFYSILSNLPTRKPYYS
MLCCCNDLLKRITINKGENLQILQYRWLLIILNIPTIRTCLIRDRKSKNVFETQQIRA
VSYELAKRCIGYLSNLSTKTSQQLIQSLRRTPTDNFSYQVEILNLYINFQFSRLLSNE
LSNRTAKNNVKPEDEMRSRLRRHHTTGHEFLSTRPISAQSNDKQGSGFTHPVNNKMKF
KFFQYEEDWHIHSAAKLTFIYYVANTRRNGRGALSIQSFYNITLDFIDYKQDFDHWRG
VAQKTKMNQLIEEWGNSTTKKCFSFCKYPFILSLGIKISIMEYEIRRIMEHEAEQAFL
ISLDKGKSVDVYFKIKVRRDVISHDSLRCIKEHQGDLLKSLRIEFVNEPGIDAGGLRK
EWFFLLTKSLFNPMNGLFIYIKESSRSWFAIDPPNFDKSKGKNSQLELYYLFGVVMGL
AIFNSTILDLQFPKALYKKLCSEPLSFEDYSELFPETSRNLIKMLNYTEDNFEDVFSL
TFETTYRNNNWILNDSKSSKEYVTVELCENGRNVPITQSNKHEFVMKWVEFYLEKSIE
PQYNKFVSGFKRVFAECNSIKLFNSEELERLVCGDEEQTKFDFKSLRSVTKYVGGFSD
DSRAVCWFWEIIESWDYPLQKKLLQFVTASDRIPATGISTIPFKISLLGSHDSDDLPL
AHTCFNEICLWNYSSKKKLELKLLWAINESEGYGFR"
gene <503936..>507301
/gene="MLO127"
/locus_tag="YJR039W"
/db_xref="GeneID:853495"
mRNA <503936..>507301
/gene="MLO127"
/locus_tag="YJR039W"
/product="Mlo127p"
/transcript_id="NM_001181697.1"
/db_xref="GeneID:853495"
CDS 503936..507301
/gene="MLO127"
/locus_tag="YJR039W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/note="Mitochondrial hypothetical protein; the authentic,
non-tagged protein is detected in highly purified
mitochondria in high-throughput studies"
/codon_start=1
/product="Mlo127p"
/protein_id="NP_012573.1"
/db_xref="GeneID:853495"
/db_xref="SGD:S000003800"
/translation="MPAGRIRIAMTDNIIVSEVDGPPDVVKCFIREEDGLEGQSRELV
VIGLEYIDVFEGVEQDEARKKLRLRTVGYTMAAFYFKDHTAPNRRFYILLKATGRLDF
VNLDYKIVKSLETGIDQVRSEPKFMFQDPLRTALVFNLSCTEIYEISTEDIFCLEETD
VKLSYVTSSPIVSIDACINFNDFLDKDVFTLSILTRAHNEVAYKLEACVCVFESKPAK
GTKWQRTTNLTFVEEATVSQVLLKSVTNIGHFVFTPWKTYFIKHALSSKQTIDGKTVD
KIYQGPGAFGSDNMERIELLQPILADTTLNHLTFTFMTSTAILITCRMNAILSSFEDD
TYIWEKALFERLPINGGSSHDHYLAAFFNEKCWILVSPKGHLTVYSVRNEGHSNFVHL
GSFLCKSTLYSDLIGNYTKSHLSCGSLHCGQGYLCLKFRSCGDIFASTCMKLLFKSKD
SVPRQVYSTRKGIYWADVNNNVYRDSERIDFEINGSFIATKDGTLLKDNTIVTLVPIQ
RDNECNYAYVTKQGYLRWSFSKVYYRIQNTGVDLTIDNCFLSAISSKGSFLTVLVLND
EITVFDHCNRLKSQKVVFHRLSDLASIFLYEYESTVYIFMSDTEGNLCVMKLATFEIV
EEVKICKKKLQFCEVPNSDYFFIYTADTIIFFKPSKIKGRFKIQEVYAPCPISCLIPG
EKDGSVVMVTSQGQFYDVLVPGDAGRATLCSKFEKVLKTCLKFITLESSSRYVIVAAL
PVANPLQDKYSEIYVYDIKQFKNISAFNFSNLNNDIESIKYENAMISDIIAVPMLKRT
ETLGKRKTSELYKEVIFNSCILVSLNLDSIDGIDSKNMNNLLLFSFDEESGFIEFVFG
INTGFSISGLHNYYNGCVLVYGEFVQAYQLNYSVHDNKFSIEQVSNRLNISGITITSS
IFFDKRKAKMARKQQNIGTWVYLEEMILLDVRKGVMRFNVIHTTDGNIEKVQLQVQPL
NLFERDLINSITDTGKMFTGAAAITFKNIRYLLISYGDQKLTLFSLKLDGEEEIDERV
YHVAEQVTTINSVRTTDSRMSTFLGESTFMPLFLVSTLSNGCYVIGILHEESDISLHI
LSEKKAVFAKRSVQKFLGFLDPQMDDHTVISEI"
gene <507746..>510085
/gene="GEF1"
/locus_tag="YJR040W"
/gene_synonym="CLC"
/db_xref="GeneID:853497"
mRNA <507746..>510085
/gene="GEF1"
/locus_tag="YJR040W"
/gene_synonym="CLC"
/product="Gef1p"
/transcript_id="NM_001181698.1"
/db_xref="GeneID:853497"
CDS 507746..510085
/gene="GEF1"
/locus_tag="YJR040W"
/gene_synonym="CLC"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:17662057]"
/experiment="EXISTENCE:direct assay:GO:0005247
voltage-gated chloride channel activity [PMID:12074596]"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:15710404]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:17662057]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:15710404]"
/experiment="EXISTENCE:direct assay:GO:0005797 Golgi
medial cisterna [PMID:9614122]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:17662057]"
/experiment="EXISTENCE:mutant phenotype:GO:0006878
intracellular copper ion homeostasis
[PMID:9520490|PMID:9614122]"
/experiment="EXISTENCE:mutant phenotype:GO:0006879
intracellular iron ion homeostasis [PMID:9520490]"
/note="Voltage-gated chloride channel; localized to the
golgi, the endosomal system, and plasma membrane; involved
in cation homeostasis; highly homologous to vertebrate
voltage-gated chloride channels; modulates TBSV model (+)
RNA virus replication by regulating copper metabolism"
/codon_start=1
/product="Gef1p"
/protein_id="NP_012574.1"
/db_xref="GeneID:853497"
/db_xref="SGD:S000003801"
/translation="MPTTYVPINQPIGDGEDVIDTNRFTNIPETQNFDQFVTIDKIAE
ENRPLSVDSDREFLNSKYRHYREVIWDRAKTFITLSSTAIVIGCIAGFLQVFTETLVN
WKTGHCQRNWLLNKSFCCNGVVNEVTSTSNLLLKRQEFECEAQGLWIAWKGHVSPFII
FMLLSVLFALISTLLVKYVAPMATGSGISEIKVWVSGFEYNKEFLGFLTLVIKSVALP
LAISSGLSVGKEGPSVHYATCCGYLLTKWLLRDTLTYSSQYEYITAASGAGVAVAFGA
PIGGVLFGLEEIASANRFNSSTLWKSYYVALVAITTLKYIDPFRNGRVILFNVTYDRD
WKVQEIPIFIALGIFGGLYGKYISKWNINFIHFRKMYLSSWPVQEVLFLATLTALISY
FNEFLKLDMTESMGILFHECVKNDNTSTFSHRLCQLDENTHAFEFLKIFTSLCFATVI
RALLVVVSYGARVPAGIFVPSMAVGATFGRAVSLLVERFISGPSVITPGAYAFLGAAA
TLSGITNLTLTVVVIMFELTGAFMYIIPLMIVVAITRIILSTSGISGGIADQMIMVNG
FPYLEDEQDEEEEETLEKYTAEQLMSSKLITINETIYLSELESLLYDSASEYSVHGFP
ITKDEDKFEKEKRCIGYVLKRHLASKIMMQSVNSTKAQTTLVYFNKSNEELGHRENCI
GFKDIMNESPISVKKAVPVTLLFRMFKELGCKTIIVEESGILKGLVTAKDILRFKRIK
YREVHGAKFTYNEALDRRCWSVIHFIIKRFTTNRNGNVI"
gene complement(<510239..>513763)
/gene="URB2"
/locus_tag="YJR041C"
/gene_synonym="NPA2"
/db_xref="GeneID:853498"
mRNA complement(<510239..>513763)
/gene="URB2"
/locus_tag="YJR041C"
/gene_synonym="NPA2"
/product="ribosome biogenesis protein URB2"
/transcript_id="NM_001181699.3"
/db_xref="GeneID:853498"
CDS complement(510239..513763)
/gene="URB2"
/locus_tag="YJR041C"
/gene_synonym="NPA2"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:15226434|PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0016072 rRNA
metabolic process [PMID:15242642]"
/experiment="EXISTENCE:physical interaction:GO:0042254
ribosome biogenesis [PMID:15226434]"
/note="Protein required for normal metabolism of the rRNA
primary transcript; nucleolar protein; proposed to be
involved in ribosome biogenesis"
/codon_start=1
/product="ribosome biogenesis protein URB2"
/protein_id="NP_012575.3"
/db_xref="GeneID:853498"
/db_xref="SGD:S000003802"
/translation="MGDLTEELSIPDNAQDLSKLLRSTSTKPHQIAEIVSKFDKLETY
FPKKEIFVLDLLIDRLNNGNLDDFKTSEHTWIIFTRLLDAINDPISIKKLLKKLKTVP
VMIRTFFLWPKDKLLTRSVSFIKAFFAINDYLIVNFSVEESFQLLEHAINGLSSCPTT
DFALSYLQDACNLTHVDNITTTDNKIATCYCKHMLLPSLRYFAQTKNSASSNQSFIRL
SHFMGKFLLQPRIDYMKLNKKFVQENASEITDDMAYYYFATFVTFLSKDNFAQLEVIF
TILGAKKPSLECRFLNLLSESKKTVSQEFLEALLLEMLASTDESGVLSLIPIILKLDI
EVAIKHIFRLLELIQLENLNDPLFSSHIWDLIIQSHANARELSDFFAKINEYCSRKGP
DSYFLINHPAYVKSITKQLFTLSSLQWKNLLQALLDQVNHDSTNRVPLYLIRICLEGL
SEGASRATLDEVKPILSQVFTLESFNNSLQWDLKYHIMEVYDDIVPAEELEKIDYVLS
SNIFDTTSADVEELFFYCFKLREYISFDLSDAKKKFMRHFEILDEERKSNLSYSVVSK
FATLVNNNFTREQISSLIDSLLLNSTNLSSLLKNDDIFEETNITYALINKLASSYHQT
FALEALIQIPIQCINKNVRVALINNLTCESFCLDSATRECLLHLLSSPTFKSNIETNF
YELCEKTIMSPEMAISETGDEKKEIEDKISIFEKVWTNHLSQAKEPVSEKFLESGYDI
VKQSMSLSNGDSKLIIAGFTIAKFLKPDNKHRDIQGMAISYAVKILENYSENFESETI
PLFRISMSTLYKIITTGQGDISKHKSRILDIFSKIMLRYHSKKVYHAPEEQEMFLVHS
LLTENKLEYIFAEYLNIEHTDKCDSALGFCLEESLKQGPDAFNRLLWNSAKSFSTISQ
PCAEKFVRVFIIMSKRIARDNNLGHHLFVIALLEAYTYCDIEKFGYKSYLLLFNAIKE
FLVSKPWLFSQYCIEMLLPFCLKTLAFIVNHESTDEINEGFINIIEVIDHMLLVHRFK
FSNRHHLFNSVLCQILEIIAIHDGTLCANSADAVARLITNYCEPYNVSNAQNGQKNNL
SSKISLIKQSIRKNVLVVLTKYIQLSITTQFSLNIKKSLQPGIHAIFDILSQNELNQL
NAFLDTPGKQYFKALYLQYKKVGKWRED"
gene <514055..>516289
/gene="NUP85"
/locus_tag="YJR042W"
/gene_synonym="RAT9"
/db_xref="GeneID:853499"
mRNA <514055..>516289
/gene="NUP85"
/locus_tag="YJR042W"
/gene_synonym="RAT9"
/product="Nup85p"
/transcript_id="NM_001181700.1"
/db_xref="GeneID:853499"
CDS 514055..516289
/gene="NUP85"
/locus_tag="YJR042W"
/gene_synonym="RAT9"
/experiment="EXISTENCE:direct assay:GO:0005643 nuclear
pore [PMID:10684247]"
/experiment="EXISTENCE:direct assay:GO:0031080 nuclear
pore outer ring [PMID:18046406|PMID:11823431]"
/experiment="EXISTENCE:direct assay:GO:0045893 positive
regulation of DNA-templated transcription [PMID:15817685]"
/experiment="EXISTENCE:mutant phenotype:GO:0000055
ribosomal large subunit export from nucleus
[PMID:11071906]"
/experiment="EXISTENCE:mutant phenotype:GO:0006406 mRNA
export from nucleus [PMID:8565072|PMID:8816998]"
/experiment="EXISTENCE:mutant phenotype:GO:0006606 protein
import into nucleus [PMID:12730220]"
/experiment="EXISTENCE:mutant phenotype:GO:0017056
structural constituent of nuclear pore [PMID:22331846]"
/experiment="EXISTENCE:mutant phenotype:GO:0051664 nuclear
pore localization [PMID:8565072|PMID:8816998]"
/note="Subunit of the Nup84p subcomplex of the nuclear
pore complex (NPC); contributes to nucleocytoplasmic
transport and NPC biogenesis and is involved in
establishment of a normal nucleocytoplasmic concentration
gradient of the GTPase Gsp1p; also plays roles in several
processes that may require localization of genes or
chromosomes at the nuclear periphery, including
double-strand break repair, transcription and chromatin
silencing; homologous to human NUP85 aka NUP75"
/codon_start=1
/product="Nup85p"
/protein_id="NP_012576.1"
/db_xref="GeneID:853499"
/db_xref="SGD:S000003803"
/translation="MTIDDSNRLLMDVDQFDFLDDGTAQLSNNKTDEEEQLYKRDPVS
GAILVPMTVNDQPIEKNGDKMPLKFKLGPLSYQNMAFITAKDKYKLYPVRIPRLDTSK
EFSAYVSGLFEIYRDLGDDRVFNVPTIGVVNSNFAKEHNATVNLAMEAILNELEVFIG
RVKDQDGRVNRFYELEESLTVLNCLRTMYFILDGQDVEENRSEFIESLLNWINRSDGE
PDEEYIEQVFSVKDSTAGKKVFETQYFWKLLNQLVLRGLLSQAIGCIERSDLLPYLSD
TCAVSFDAVSDSIELLKQYPKDSSSTFREWKNLVLKLSQAFGSSATDISGELRDYIED
FLLVIGGNQRKILQYSRTWYESFCGFLLYYIPSLELSAEYLQMSLEANVVDITNDWEQ
PCVDIISGKIHSILPVMESLDSCTAAFTAMICEAKGLIENIFEGEKNSDDYSNEDNEM
LEDLFSYRNGMASYMLNSFAFELCSLGDKELWPVAIGLIALSATGTRSAKKMVIAELL
PHYPFVTNDDIEWMLSICVEWRLPEIAKEIYTTLGNQMLSAHNIIESIANFSRAGKYE
LVKSYSWLLFEASCMEGQKLDDPVLNAIVSKNSPAEDDVIIPQDILDCVVTNSMRQTL
APYAVLSQFYELRDREDWGQALRLLLLLIEFPYLPKHYLVLLVAKFLYPIFLLDDKKL
MDEDSVATVIEVIETKWDDADEKSSNLYETIIEADKSLPSSMATLLKNLRKKLNFKLC
QAFM"
gene complement(<516461..>517513)
/gene="POL32"
/locus_tag="YJR043C"
/gene_synonym="REV5"
/db_xref="GeneID:853500"
mRNA complement(<516461..>517513)
/gene="POL32"
/locus_tag="YJR043C"
/gene_synonym="REV5"
/product="DNA polymerase delta subunit POL32"
/transcript_id="NM_001181701.1"
/db_xref="GeneID:853500"
CDS complement(516461..517513)
/gene="POL32"
/locus_tag="YJR043C"
/gene_synonym="REV5"
/experiment="EXISTENCE:direct assay:GO:0000510 H3-H4
histone complex chaperone activity [PMID:38713623]"
/experiment="EXISTENCE:direct assay:GO:0003887
DNA-directed DNA polymerase activity [PMID:18635534]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0006278
RNA-templated DNA biosynthetic process [PMID:17429354]"
/experiment="EXISTENCE:direct assay:GO:0016035 zeta DNA
polymerase complex [PMID:23066099|PMID:22711820]"
/experiment="EXISTENCE:direct assay:GO:0043137 DNA
replication, removal of RNA primer [PMID:16837458]"
/experiment="EXISTENCE:mutant phenotype:GO:0000510 H3-H4
histone complex chaperone activity [PMID:38713623]"
/experiment="EXISTENCE:mutant phenotype:GO:0000727
double-strand break repair via break-induced replication
[PMID:17671506]"
/experiment="EXISTENCE:mutant phenotype:GO:0006277 DNA
amplification [PMID:23271978]"
/experiment="EXISTENCE:mutant phenotype:GO:0042276
error-prone translesion synthesis [PMID:17681555]"
/note="Third subunit of DNA polymerase delta; involved in
chromosomal DNA replication; required for error-prone DNA
synthesis in the presence of DNA damage and processivity;
parental H3-H4 histone chaperone that mediates transfer of
parental histones to the lagging strand via Pol1p during
DNA replication; forms a complex with Rev3p, Rev7p and
Pol31p; interacts with Hys2p, PCNA (Pol30p), and Pol1p"
/codon_start=1
/product="DNA polymerase delta subunit POL32"
/protein_id="NP_012577.1"
/db_xref="GeneID:853500"
/db_xref="SGD:S000003804"
/translation="MDQKASYFINEKLFTEVKPVLFTDLIHHLKIGPSMAKKLMFDYY
KQTTNAKYNCVVICCYKDQTIKIIHDLSNIPQQDSIIDCFIYAFNPMDSFIPYYDIID
QKDCLTIKNSYELKVSESSKIIERTKTLEEKSKPLVRPTARSKTTPEETTGRKSKSKD
MGLRSTALLAKMKKDRDDKETSRQNELRKRKEENLQKINKQNPEREAQMKELNNLFVE
DDLDTEEVNGGSKPNSPKETDSNDKDKNNDDLEDLLETTAEDSLMDVPKIQQTKPSET
EHSKEPKSEEEPSSFIDEDGYIVTKRPATSTPPRKPSPVVKRALSSSKKQETPSSNKR
LKKQGTLESFFKRKAK"
gene complement(517813..517884)
/gene="IMT3"
/locus_tag="YNCJ0022C"
/db_xref="GeneID:853501"
tRNA complement(517813..517884)
/gene="IMT3"
/locus_tag="YNCJ0022C"
/product="tRNA-Met"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006413
translational initiation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0016282 eukaryotic
43S preinitiation complex [PMID:12008673]"
/experiment="EXISTENCE:direct assay:GO:0033290 eukaryotic
48S preinitiation complex [PMID:17242201]"
/experiment="EXISTENCE:direct assay:GO:0071074 eukaryotic
initiation factor eIF2 binding [PMID:14698289]"
/note="Methionine initiator tRNA (tRNA-Met); predicted by
tRNAscan-SE analysis; one of four initiator methionine
tRNAs in yeast that are functional for translation"
/db_xref="GeneID:853501"
/db_xref="SGD:S000006663"
repeat_region 517959..518099
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007043"
gene complement(<518763..>519185)
/gene="VPS55"
/locus_tag="YJR044C"
/db_xref="GeneID:853502"
mRNA complement(<518763..>519185)
/gene="VPS55"
/locus_tag="YJR044C"
/product="Vps55p"
/transcript_id="NM_001181702.3"
/db_xref="GeneID:853502"
CDS complement(518763..519185)
/gene="VPS55"
/locus_tag="YJR044C"
/experiment="EXISTENCE:direct assay:GO:0005770 late
endosome [PMID:12006663]"
/experiment="EXISTENCE:direct assay:GO:0034424 Vps55/Vps68
complex [PMID:18216282]"
/experiment="EXISTENCE:mutant phenotype:GO:0032511 late
endosome to vacuole transport via multivesicular body
sorting pathway [PMID:18216282]"
/experiment="EXISTENCE:physical interaction:GO:0034424
Vps55/Vps68 complex [PMID:18216282]"
/note="Late endosomal protein involved in late endosome to
vacuole transport; functional homolog of human obesity
receptor gene-related protein (OB-RGRP)"
/codon_start=1
/product="Vps55p"
/protein_id="NP_012578.3"
/db_xref="GeneID:853502"
/db_xref="SGD:S000003805"
/translation="MMEFKVSPLTKIISLSGFLALGFLLVILSCALFHNYYPLFDILI
FLLAPIPNTIFNAGNKYHTSDFMSDSSNTGQDLAHFLTGMLVTSGIALPVVFYHCQLI
GHLSCIMCMIGGLIIYSSIVIFKWFFKKDFNEDDSLFG"
gene complement(<519638..>521602)
/gene="SSC1"
/locus_tag="YJR045C"
/gene_synonym="ENS1"
/db_xref="GeneID:853503"
mRNA complement(<519638..>521602)
/gene="SSC1"
/locus_tag="YJR045C"
/gene_synonym="ENS1"
/product="Hsp70 family ATPase SSC1"
/transcript_id="NM_001181703.1"
/db_xref="GeneID:853503"
CDS complement(519638..521602)
/gene="SSC1"
/locus_tag="YJR045C"
/gene_synonym="ENS1"
/EC_number="3.6.4.10"
/experiment="EXISTENCE:direct assay:GO:0001405 PAM
complex, Tim23 associated import motor
[PMID:14517234|PMID:14638855]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:16823961|PMID:11914276|PMID:14576278|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:7935837]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:11096111]"
/experiment="EXISTENCE:direct assay:GO:0030150 protein
import into mitochondrial matrix [PMID:8654364]"
/experiment="EXISTENCE:direct assay:GO:0030234 enzyme
regulator activity [PMID:10464305]"
/experiment="EXISTENCE:direct assay:GO:0042026 protein
refolding [PMID:9973563]"
/experiment="EXISTENCE:direct assay:GO:0042645
mitochondrial nucleoid [PMID:15692048]"
/experiment="EXISTENCE:mutant phenotype:GO:0030150 protein
import into mitochondrial matrix [PMID:10779357]"
/experiment="EXISTENCE:mutant phenotype:GO:0042026 protein
refolding [PMID:16460754]"
/experiment="EXISTENCE:mutant phenotype:GO:0043335 protein
unfolding [PMID:8408192]"
/note="Hsp70 family ATPase; constituent of the import
motor component of the Translocase of the Inner
Mitochondrial membrane (TIM23 complex); involved in
protein translocation and folding; subunit of SceI
endonuclease; SSC1 has a paralog, ECM10, that arose from
the whole genome duplication"
/codon_start=1
/product="Hsp70 family ATPase SSC1"
/protein_id="NP_012579.1"
/db_xref="GeneID:853503"
/db_xref="SGD:S000003806"
/translation="MLAAKNILNRSSLSSSFRIATRLQSTKVQGSVIGIDLGTTNSAV
AIMEGKVPKIIENAEGSRTTPSVVAFTKEGERLVGIPAKRQAVVNPENTLFATKRLIG
RRFEDAEVQRDIKQVPYKIVKHSNGDAWVEARGQTYSPAQIGGFVLNKMKETAEAYLG
KPVKNAVVTVPAYFNDSQRQATKDAGQIVGLNVLRVVNEPTAAALAYGLEKSDSKVVA
VFDLGGGTFDISILDIDNGVFEVKSTNGDTHLGGEDFDIYLLREIVSRFKTETGIDLE
NDRMAIQRIREAAEKAKIELSSTVSTEINLPFITADASGPKHINMKFSRAQFETLTAP
LVKRTVDPVKKALKDAGLSTSDISEVLLVGGMSRMPKVVETVKSLFGKDPSKAVNPDE
AVAIGAAVQGAVLSGEVTDVLLLDVTPLSLGIETLGGVFTRLIPRNTTIPTKKSQIFS
TAAAGQTSVEIRVFQGERELVRDNKLIGNFTLAGIPPAPKGVPQIEVTFDIDADGIIN
VSARDKATNKDSSITVAGSSGLSENEIEQMVNDAEKFKSQDEARKQAIETANKADQLA
NDTENSLKEFEGKVDKAEAQKVRDQITSLKELVARVQGGEEVNAEELKTKTEELQTSS
MKLFEQLYKNDSNNNNNNNGNNAESGETKQ"
gene <522048..>523862
/gene="TAH11"
/locus_tag="YJR046W"
/gene_synonym="CDT1; SID2"
/db_xref="GeneID:853504"
mRNA <522048..>523862
/gene="TAH11"
/locus_tag="YJR046W"
/gene_synonym="CDT1; SID2"
/product="Tah11p"
/transcript_id="NM_001181704.1"
/db_xref="GeneID:853504"
CDS 522048..523862
/gene="TAH11"
/locus_tag="YJR046W"
/gene_synonym="CDT1; SID2"
/experiment="EXISTENCE:direct assay:GO:0003688 DNA
replication origin binding [PMID:16824194]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11836525]"
/experiment="EXISTENCE:direct assay:GO:0005656 nuclear
pre-replicative complex [PMID:16824194]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11836525]"
/experiment="EXISTENCE:direct assay:GO:0006267
pre-replicative complex assembly involved in nuclear cell
cycle DNA replication [PMID:16824194]"
/experiment="EXISTENCE:direct assay:GO:0030174 regulation
of DNA-templated DNA replication initiation
[PMID:11967159]"
/experiment="EXISTENCE:mutant phenotype:GO:0000727
double-strand break repair via break-induced replication
[PMID:20516198]"
/experiment="EXISTENCE:mutant phenotype:GO:0005656 nuclear
pre-replicative complex [PMID:11967159]"
/note="DNA replication licensing factor; required for
pre-replication complex assembly"
/codon_start=1
/product="Tah11p"
/protein_id="NP_012580.1"
/db_xref="GeneID:853504"
/db_xref="SGD:S000003807"
/translation="MSGTANSRRKEVLRVPVIDLNRVSDEEQLLPVVRAILLQHDTFL
LKNYANKAVLDALLAGLTTKDLPDTSQGFDANFTGTLPLEDDVWLEQYIFDTDPQLRF
DRKCRNESLCSIYSRLFKLGLFFAQLCVKSVVSSAELQDCISTSHYATKLTRYFNDNG
STHDGADAGATVLPTGDDFQYLFERDYVTFLPTGVLTIFPCAKAIRYKPSTMATTDNS
WVSIDEPDCLLFHTGTLLARWSQGMHTTSPLQIDPRANIVSLTIWPPLTTPISSKGEG
TIANHLLEQQIKAFPKVAQQYYPRELSILRLQDAMKFVKELFTVCETVLSLNALSRST
GVPPELHVLLPQISSMMKRKIVQDDILKLLTIWSDAYVVELNSRGELTMNLPKRDNLT
TLTNKSRTLAFVERAESWYQQVIASKDEIMTDVPAFKINKRRSSSNSKTVLSSKVQTK
SSNANALNNSRYLANSKENFMYKEKMPDSQANLMDRLRERERRSAALLSQRQKRYQQF
LAMKMTQVFDILFSLTRGQPYTETYLSSLIVDSLQDSNNPIGTKEASEILAGLQGILP
MDISVHQVDGGLKVYRWNSLDKNRFSKLLQIHKSKQQD"
gene complement(524012..524093)
/locus_tag="YNCJ0023C"
/db_xref="GeneID:853505"
tRNA complement(524012..524093)
/locus_tag="YNCJ0023C"
/product="tRNA-Ser"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0030371 translation
repressor activity [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043555 regulation
of translation in response to stress [PMID:27609601]"
/note="Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:853505"
/db_xref="SGD:S000006727"
gene complement(<524908..>525381)
/gene="ANB1"
/locus_tag="YJR047C"
/gene_synonym="HYP1; TIF51B"
/db_xref="GeneID:853506"
mRNA complement(<524908..>525381)
/gene="ANB1"
/locus_tag="YJR047C"
/gene_synonym="HYP1; TIF51B"
/product="translation elongation factor eIF-5A"
/transcript_id="NM_001181705.3"
/db_xref="GeneID:853506"
CDS complement(524908..525381)
/gene="ANB1"
/locus_tag="YJR047C"
/gene_synonym="HYP1; TIF51B"
/experiment="EXISTENCE:direct assay:GO:0022626 cytosolic
ribosome [PMID:27196944]"
/experiment="EXISTENCE:genetic interaction:GO:0006452
translational frameshifting [PMID:19424157]"
/experiment="EXISTENCE:genetic interaction:GO:0045901
positive regulation of translational elongation
[PMID:19424157]"
/note="Translation elongation factor eIF-5A; previously
thought to function in translation initiation; undergoes
an essential hypusination modification; expressed under
anaerobic conditions; ANB1 has a paralog, HYP2, that arose
from the whole genome duplication; human EIF5A complements
the inviability of the yeast hyp2 anb1 double null mutant"
/codon_start=1
/product="translation elongation factor eIF-5A"
/protein_id="NP_012581.3"
/db_xref="GeneID:853506"
/db_xref="SGD:S000003808"
/translation="MSDEEHTFENADAGASATYPMQCSALRKNGFVVIKGRPCKIVDM
STSKTGKHGHAKVHLVTLDIFTGKKLEDLSPSTHNLEVPFVKRSEYQLLDIDDGYLSL
MTMDGETKDDVKAPEGELGDSMQAAFDEGKDLMVTIISAMGEEAAISFKEAPRSD"
gene <526335..>526664
/gene="CYC1"
/locus_tag="YJR048W"
/db_xref="GeneID:853507"
mRNA <526335..>526664
/gene="CYC1"
/locus_tag="YJR048W"
/product="cytochrome c isoform 1"
/transcript_id="NM_001181706.1"
/db_xref="GeneID:853507"
CDS 526335..526664
/gene="CYC1"
/locus_tag="YJR048W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005758
mitochondrial intermembrane space [PMID:9866716]"
/experiment="EXISTENCE:direct assay:GO:0006122
mitochondrial electron transport, ubiquinol to cytochrome
c [PMID:7851399]"
/experiment="EXISTENCE:direct assay:GO:0006123
mitochondrial electron transport, cytochrome c to oxygen
[PMID:7851399]"
/experiment="EXISTENCE:direct assay:GO:0009055 electron
transfer activity [PMID:7851399|PMID:18975895]"
/experiment="EXISTENCE:direct assay:GO:1901612 cardiolipin
binding [PMID:30182710]"
/note="Cytochrome c, isoform 1; also known as
iso-1-cytochrome c; electron carrier of mitochondrial
intermembrane space that transfers electrons from
ubiquinone-cytochrome c oxidoreductase to cytochrome c
oxidase during cellular respiration; CYC1 has a paralog,
CYC7, that arose from the whole genome duplication; human
homolog CYC1 can complement yeast null mutant; mutations
in human CYC1 cause insulin-responsive hyperglycemia"
/codon_start=1
/product="cytochrome c isoform 1"
/protein_id="NP_012582.1"
/db_xref="GeneID:853507"
/db_xref="SGD:S000003809"
/translation="MTEFKAGSAKKGATLFKTRCLQCHTVEKGGPHKVGPNLHGIFGR
HSGQAEGYSYTDANIKKNVLWDENNMSEYLTNPKKYIPGTKMAFGGLKKEKDRNDLIT
YLKKACE"
gene complement(<526884..>528476)
/gene="UTR1"
/locus_tag="YJR049C"
/db_xref="GeneID:853508"
mRNA complement(<526884..>528476)
/gene="UTR1"
/locus_tag="YJR049C"
/product="NADH/NAD(+) kinase"
/transcript_id="NM_001181707.1"
/db_xref="GeneID:853508"
CDS complement(526884..528476)
/gene="UTR1"
/locus_tag="YJR049C"
/EC_number="2.7.1.23"
/experiment="EXISTENCE:direct assay:GO:0003951 NAD+ kinase
activity [PMID:11425472]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006741 NADP+
biosynthetic process [PMID:15978040|PMID:11425472]"
/experiment="EXISTENCE:direct assay:GO:0042736 NADH kinase
activity [PMID:15978040]"
/experiment="EXISTENCE:mutant phenotype:GO:0006879
intracellular iron ion homeostasis [PMID:8662826]"
/note="ATP-NADH kinase; phosphorylates both NAD and NADH;
active as a hexamer; enhances the activity of ferric
reductase (Fre1p); UTR1 has a paralog, YEF1, that arose
from the whole genome duplication"
/codon_start=1
/product="NADH/NAD(+) kinase"
/protein_id="NP_012583.1"
/db_xref="GeneID:853508"
/db_xref="SGD:S000003810"
/translation="MKENDMNNGVDKWVNEEDGRNDHHNNNNNLMKKAMMNNEQIDRT
QDIDNAKEMLRKISSESSSRRSSLLNKDSSLVNGNANSGGGTSINGTRGSSKSSNTHF
QYASTAYGVRMLSKDISNTKVELDVENLMIVTKLNDVSLYFLTRELVEWVLVHFPRVT
VYVDSELKNSKKFAAGELCEDSKCRESRIKYWTKDFIREHDVFFDLVVTLGGDGTVLF
VSSIFQRHVPPVMSFSLGSLGFLTNFKFEHFREDLPRIMNHKIKTNLRLRLECTIYRR
HRPEVDPNTGKKICVVEKLSTHHILNEVTIDRGPSPFLSMLELYGDGSLMTVAQADGL
IAATPTGSTAYSLSAGGSLVCPTVNAIALTPICPHALSFRPIILPESINLKVKVSMKS
RAPAWAAFDGKDRIELQKGDFITICASPYAFPTVEASPDEFINSISRQLNWNVREQQK
SFTHILSQKNQEKYAHEANKVRNQAEPLEVIRDKYSLEADATKENNNGSDDESDDESV
NCEACKLKPSSVPKPSQARFSV"
gene <528697..>529404
/gene="ISY1"
/locus_tag="YJR050W"
/gene_synonym="NTC30; UTR3"
/db_xref="GeneID:853509"
mRNA <528697..>529404
/gene="ISY1"
/locus_tag="YJR050W"
/gene_synonym="NTC30; UTR3"
/product="Isy1p"
/transcript_id="NM_001181708.3"
/db_xref="GeneID:853509"
CDS 528697..529404
/gene="ISY1"
/locus_tag="YJR050W"
/gene_synonym="NTC30; UTR3"
/experiment="EXISTENCE:direct assay:GO:0000974 Prp19
complex [PMID:11018040]"
/experiment="EXISTENCE:direct assay:GO:0071006 U2-type
catalytic step 1 spliceosome
[PMID:10094305|PMID:11018040]"
/experiment="EXISTENCE:direct assay:GO:0071007 U2-type
catalytic step 2 spliceosome [PMID:10094305]"
/experiment="EXISTENCE:direct assay:GO:0071014 post-mRNA
release spliceosomal complex [PMID:10094305]"
/experiment="EXISTENCE:direct assay:GO:0071020
post-spliceosomal complex [PMID:10094305]"
/experiment="EXISTENCE:genetic interaction:GO:0000384
first spliceosomal transesterification activity
[PMID:16103217]"
/experiment="EXISTENCE:mutant phenotype:GO:0000350
generation of catalytic spliceosome for second
transesterification step [PMID:10094305]"
/experiment="EXISTENCE:mutant phenotype:GO:0000389 mRNA
3'-splice site recognition [PMID:16103217]"
/note="Member of the NineTeen Complex (NTC); NTC contains
Prp19p and stabilizes U6 snRNA in catalytic forms of
spliceosome containing U2, U5, and U6 snRNAs; interacts
with Prp16p to modulate splicing fidelity; isy1 syf2 cells
have defective spindles"
/codon_start=1
/product="Isy1p"
/protein_id="NP_012584.3"
/db_xref="GeneID:853509"
/db_xref="SGD:S000003811"
/translation="MSRNVDKANSVLVRFQEQQAESAGGYKDYSRYQRPRNVSKVKSI
KEANEWKRQVSKEIKQKSTRIYDPSLNEMQIAELNDELNNLFKEWKRWQWHIDHTLME
KKTKRKRLEDSHVLMNSGKLINGKRYFGRALELPEVKEWLKQSQRQNDGGSINTKCIP
KDRNDFYYHGKVTAALTEFEANWTSILKAHYNVPVNEDEEEMSRQTQEIHVPTLADME
HWLVQRRKKKLMDELNL"
gene <529861..>531366
/gene="OSM1"
/locus_tag="YJR051W"
/db_xref="GeneID:853510"
mRNA <529861..>531366
/gene="OSM1"
/locus_tag="YJR051W"
/product="fumarate reductase"
/transcript_id="NM_001181709.1"
/db_xref="GeneID:853510"
CDS 529861..531366
/gene="OSM1"
/locus_tag="YJR051W"
/EC_number="1.3.1.6"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:9587404|PMID:24769239|PMID:25378625|PMID:16823961|PM
ID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:25378625]"
/experiment="EXISTENCE:direct assay:GO:0016156 fumarate
reductase (NADH) activity [PMID:9587404]"
/experiment="EXISTENCE:mutant phenotype:GO:0034975 protein
folding in endoplasmic reticulum [PMID:25378625]"
/experiment="EXISTENCE:mutant phenotype:GO:0046443 FAD
metabolic process [PMID:17345583]"
/note="Fumarate reductase, catalyzes the reduction of
fumarate to succinate; required for the reoxidation of
intracellular NADH under anaerobic conditions; acts as
electron acceptor in mitochondrial intermembrane space;
has two translation start sites, one at the annotated
start codon which produces an ER-targeted form required
for anaerobic growth, and one at codon 32 which produces a
mitochondrially-targeted form; OSM1 has a paralog, FRD1,
that arose from the whole genome duplication"
/codon_start=1
/product="fumarate reductase"
/protein_id="NP_012585.1"
/db_xref="GeneID:853510"
/db_xref="SGD:S000003812"
/translation="MIRSVRRVFIYVSIFVLIIVLKRTLSGTDQTSMKQPVVVIGSGL
AGLTTSNRLISKYRIPVVLLDKAASIGGNSIKASSGINGAHTDTQQNLKVMDTPELFL
KDTLHSAKGRGVPSLMDKLTKESKSAIRWLQTEFDLKLDLLAQLGGHSVPRTHRSSGK
LPPGFEIVQALSKKLKDISSKDSNLVQIMLNSEVVDIELDNQGHVTGVVYMDENGNRK
IMKSHHVVFCSGGFGYSKEMLKEYSPNLIHLPTTNGKQTTGDGQKILSKLGAELIDMD
QVQVHPTGFIDPNDRENNWKFLAAEALRGLGGILLHPTTGRRFTNELSTRDTVTMEIQ
SKCPKNDNRALLVMSDKVYENYTNNINFYMSKNLIKKVSINDLIRQYDLQTTASELVT
ELKSYSDVNTKDTFDRPLIINAFDKDISTESTVYVGEVTPVVHFTMGGVKINEKSQVI
KKNSESVLSNGIFAAGEVSGGVHGANRLGGSSLLECVVFGKTAADNIAKLY"
repeat_region complement(531516..531675)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007032"
gene 531828..531898
/gene="SUF23"
/locus_tag="YNCJ0024W"
/db_xref="GeneID:853511"
tRNA 531828..531898
/gene="SUF23"
/locus_tag="YNCJ0024W"
/product="tRNA-Gly"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0030371 translation
repressor activity [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043555 regulation
of translation in response to stress [PMID:27609601]"
/note="Glycine tRNA (tRNA-Gly); can mutate to suppress +1
frameshift mutations"
/db_xref="GeneID:853511"
/db_xref="SGD:S000006584"
gene <532062..>533759
/gene="RAD7"
/locus_tag="YJR052W"
/db_xref="GeneID:853512"
mRNA <532062..>533759
/gene="RAD7"
/locus_tag="YJR052W"
/product="UV-damaged DNA-binding protein RAD7"
/transcript_id="NM_001181710.1"
/db_xref="GeneID:853512"
CDS 532062..533759
/gene="RAD7"
/locus_tag="YJR052W"
/experiment="EXISTENCE:direct assay:GO:0000113
nucleotide-excision repair factor 4 complex
[PMID:9497356]"
/experiment="EXISTENCE:direct assay:GO:0000715
nucleotide-excision repair, DNA damage recognition
[PMID:15177043]"
/experiment="EXISTENCE:direct assay:GO:0003684 damaged DNA
binding [PMID:9497356]"
/experiment="EXISTENCE:direct assay:GO:0004842
ubiquitin-protein transferase activity [PMID:16675952]"
/experiment="EXISTENCE:direct assay:GO:0008094
ATP-dependent activity, acting on DNA [PMID:9497356]"
/experiment="EXISTENCE:direct assay:GO:0008104
intracellular protein localization [PMID:15226437]"
/experiment="EXISTENCE:direct assay:GO:0031463 Cul3-RING
ubiquitin ligase complex [PMID:16675952]"
/experiment="EXISTENCE:direct assay:GO:0034644 cellular
response to UV [PMID:16675952]"
/experiment="EXISTENCE:mutant phenotype:GO:0000715
nucleotide-excision repair, DNA damage recognition
[PMID:15177043]"
/experiment="EXISTENCE:mutant phenotype:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:16675952]"
/note="Nucleotide excision repair (NER) protein; binds
damaged DNA during NER; binds DNA in an ATP-dependent
manner (with Rad16p) during NER; required for repair of
non-transcribed chromatin; subunit of Nucleotide Excision
Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS)
ligase complex"
/codon_start=1
/product="UV-damaged DNA-binding protein RAD7"
/protein_id="NP_012586.1"
/db_xref="GeneID:853512"
/db_xref="SGD:S000003813"
/translation="MYRSRNRPKRGGENEVKGPNSALTQFLREEGISAENIKQKWYQR
QSKKQEDATDEKKGKAEDDSFTAEISRVVEDEEIDEIGTGSGTETERAQVSYDARMKL
VPADSDEEEYETSHISDTPVSLSSANDRESLTKKRQNTAKIIQNRRRKRKRAADLLDR
RVNKVSSLQSLCITKISENISKWQKEADESSKLVFNKLRDVLGGVSTANLNNLAKALS
KNRALNDHTLQLFLKTDLKRLTFSDCSKISFDGYKTLAIFSPHLTELSLQMCGQLNHE
SLLYIAEKLPNLKSLNLDGPFLINEDTWEKFFVIMKGRLEEFHISNTHRFTDKSLSNL
LINCGSTLVSLGLSRLDSISNYALLPQYLVNDEFHSLCIEYPFNEEDVNDEIIINLLG
QIGRTLRKLVLNGCIDLTDSMIINGLTAFIPEKCPLEVLSLEESDQITTDSLSYFFSK
VELNNLIECSFRRCLQLGDMAIIELLLNGARDSLRSLNLNSLKELTKEAFVALACPNL
TYLDLGFVRCVDDSVIQMLGEQNPNLTVIDVFGDNLVTEKATMRPGLTLIGRQSDSI"
gene <534027..>535751
/gene="BFA1"
/locus_tag="YJR053W"
/gene_synonym="IBD1"
/db_xref="GeneID:853513"
mRNA <534027..>535751
/gene="BFA1"
/locus_tag="YJR053W"
/gene_synonym="IBD1"
/product="Bfa1p"
/transcript_id="NM_001181711.3"
/db_xref="GeneID:853513"
CDS 534027..535751
/gene="BFA1"
/locus_tag="YJR053W"
/gene_synonym="IBD1"
/experiment="EXISTENCE:direct assay:GO:0005092
GDP-dissociation inhibitor activity [PMID:19948498]"
/experiment="EXISTENCE:direct assay:GO:0005096 GTPase
activator activity
[PMID:12048186|PMID:16682821|PMID:18667533]"
/experiment="EXISTENCE:direct assay:GO:0005816 spindle
pole body [PMID:15147270|PMID:10220406]"
/experiment="EXISTENCE:direct assay:GO:1990334 Bfa1-Bub2
complex [PMID:25658911]"
/experiment="EXISTENCE:mutant phenotype:GO:0001100
negative regulation of exit from mitosis
[PMID:15147270|PMID:18667533]"
/experiment="EXISTENCE:mutant phenotype:GO:0031578 mitotic
spindle orientation checkpoint signaling [PMID:16039591]"
/experiment="EXISTENCE:physical interaction:GO:1990334
Bfa1-Bub2 complex [PMID:11493673]"
/note="Subunit of a two-component GTPase-activating
protein, Bfa1p-Bub2p; contributes to GAP activity,
inactivating Tem1 by stimulating GTP hydrolysis following
damage or misalignment of the mitotic spindle; functions
as a guanine-nucleotide exchange inhibitor (GDI) for
Tem1p; involved in multiple cell cycle checkpoint pathways
that control mitotic exit; required when telomeres are
damaged, but not for all types of chromosomal DNA damage;
phosphorylated by the Polo-like kinase Cdc5p"
/codon_start=1
/product="Bfa1p"
/protein_id="NP_012587.3"
/db_xref="GeneID:853513"
/db_xref="SGD:S000003814"
/translation="MSIRPLTLNGLDEPETSFEELNTTLPRFQSHETLTLEENVPPLS
TSTYIPPPSSVGTSDTGTVFSNSTSAFWSNKQADDDQDMEVDQDDEFLNDFQEFQNKK
DDFDDAIKTNFHLRNGCRTGPFKNDIFAEEFDRKLSLEDKPRLKQPRSMMELKPKRKL
SNSVTSRNLRSGNSVRFKKSMPNLALVNPAIREEEEDEEREREDQREFNYKIDNDTQD
TILAKFSSDDEGDFLTGFEELEGEAIDETISSNDKESADHPRFLKKSSSSLPLKISPA
QYDIVKHDELLTPGLHRRQRDWNTQQELDSFKEKRSVRHCSNQNVQLNGPAKIKTIKQ
QIDHNTPMKKGSMIYNPKTMKWEGNENVLSKFSDVDTANRKALLIKNKLQRDADSKKQ
KYSDLQHARATSRNQKVIGNMILDEQNLRWVSVSEEEADPFAGIPEINLPPVGKSMKK
RSSSPFLRSKSQVNTPFVSNDNDGVYQSTAAQARLRKYHSMRTLNGTTETPEISSTFH
LSSRALEKFYHEENRWCKKLASWFIPRDETIISVDEETIMDESTVNSKRKSYMYEIRN
MVINSTKD"
gene <536056..>537549
/gene="KCH1"
/locus_tag="YJR054W"
/db_xref="GeneID:853514"
mRNA <536056..>537549
/gene="KCH1"
/locus_tag="YJR054W"
/product="Kch1p"
/transcript_id="NM_001181712.1"
/db_xref="GeneID:853514"
CDS 536056..537549
/gene="KCH1"
/locus_tag="YJR054W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:23204190]"
/experiment="EXISTENCE:direct assay:GO:0005937 mating
projection [PMID:23204190]"
/experiment="EXISTENCE:direct assay:GO:0015079 potassium
ion transmembrane transporter activity [PMID:23204190]"
/experiment="EXISTENCE:direct assay:GO:0071805 potassium
ion transmembrane transport [PMID:23204190]"
/note="Potassium transporter that mediates K+ influx;
activates high-affinity Ca2+ influx system (HACS) during
mating pheromone response; expression up-regulated in
response to alpha factor; localized to sites of polarized
growth; member of a fungal-specific gene family; potential
Cdc28p substrate; KCH1 has a paralog, PRM6, that arose
from the whole genome duplication"
/codon_start=1
/product="Kch1p"
/protein_id="NP_012588.1"
/db_xref="GeneID:853514"
/db_xref="SGD:S000003815"
/translation="MFNHDWKYSINSKTFADLNIELFRNHKFKTVLNYIIGVVGWNGL
KLALFVSDIYTCIKLLAFNSWSNNIIKPYLPFKISKWLFSGCILASIVLLIWEAIAGM
RIYKTGNISLTYVNNFSRNLNSVLNYSKFCVYNMIERKGFRQKMTFFTFFQLKDCIRL
IFTDTPRQVINGLTLWSVLVTVNKNEDLGDLESFTGLINKIKNIGQTNHEEAVILSLM
LFSFIIWALFVFKFLLAVICSIFVYYKIINDQEYSGLREYICVTVSENVDELVERQRK
KENDDTIYKTGLLESQTFDDFKEVENKIETSFNDTSYASNNDSMIELIERRPEYKSQD
VCGPIPTMKKTETMESFVDNGNPQYTTRFSAILDSPYINSYESNDIKKAKIQSRSVNT
PKYEDLSSSDIFNKIHSAGQLKSTTSMEFHGPLDSMPNTTNNIRNFNSNSSRPRPPPL
QTKSSINSKADSNDNGRIYTPMKAYFREPDLPRKGLLEDEDRTYNYT"
repeat_region complement(537741..538041)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007033"
repeat_region 538103..538434
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007044"
gene 538555..538626
/gene="HSX1"
/locus_tag="YNCJ0025W"
/db_xref="GeneID:853515"
tRNA 538555..538626
/gene="HSX1"
/locus_tag="YNCJ0025W"
/product="tRNA-Arg"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE
analysis; low abundance tRNA required for growth on
nonfermentable carbon sources at high temperatures, for
synthesis of heat shock protein Ssc1p, and for Ty1
retrotransposition through the regulation of translational
frameshifting"
/db_xref="GeneID:853515"
/db_xref="SGD:S000006707"
gene <538772..>539266
/gene="HIT1"
/locus_tag="YJR055W"
/db_xref="GeneID:853516"
mRNA <538772..>539266
/gene="HIT1"
/locus_tag="YJR055W"
/product="Hit1p"
/transcript_id="NM_001181713.1"
/db_xref="GeneID:853516"
CDS 538772..539266
/gene="HIT1"
/locus_tag="YJR055W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0000463
maturation of LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:25170085]"
/experiment="EXISTENCE:mutant phenotype:GO:0000492 box C/D
snoRNP assembly [PMID:25170085]"
/note="Protein involved in C/D snoRNP assembly; critical
for formation of small nucleolar ribonucleoprotein
complexes that are required for rRNA processing and
2'-O-methylation; regulates abundance of Rsa1p; required
for growth at high temperature; mutations in human homolog
ZNHIT3 cause PEHO syndrome, a ribosomopathy characterized
by extreme cerebellar atrophy"
/codon_start=1
/product="Hit1p"
/protein_id="NP_012589.1"
/db_xref="GeneID:853516"
/db_xref="SGD:S000003816"
/translation="MVSSAVKCGICRGVDGKYKCPKCGVRYCSLKCYKDAAKHVHKES
EQPRAGTEANVEVVNNDKIINSSLAMNKTLKTKAFDDIYQNSAELQELLKYNTVKFHL
AKVYRILSSTVNDGSSGKMNSDLQKELAVNYLNTLRYGGIHYNEAIEEFCQILLDKLN
AVKK"
rep_origin 540546..540780
/note="ARS1018; Autonomously Replicating Sequence"
/db_xref="SGD:S000028455"
repeat_region 540766..541087
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007045"
repeat_region complement(541095..541427)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007034"
gene 541508..541579
/locus_tag="YNCJ0026W"
/db_xref="GeneID:853517"
tRNA 541508..541579
/locus_tag="YNCJ0026W"
/product="tRNA-Asp"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:853517"
/db_xref="SGD:S000006539"
gene complement(<541792..>542502)
/locus_tag="YJR056C"
/db_xref="GeneID:853518"
mRNA complement(<541792..>542502)
/locus_tag="YJR056C"
/product="uncharacterized protein"
/transcript_id="NM_001181714.3"
/db_xref="GeneID:853518"
CDS complement(541792..542502)
/locus_tag="YJR056C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:22842922]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to both the cytoplasm and
the nucleus; relative distribution to the nucleus
increases upon DNA replication stress"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_012590.3"
/db_xref="GeneID:853518"
/db_xref="SGD:S000003817"
/translation="MEQMHSLESSLPPEQPPTKQAIESLNLELSQEFKLAANAVTRLY
RVANEKNSLTKHQGYLTCLDDILCALDSNVTADELRAWCYKRRNDILSNSQDKSLNPV
KERERKLNKFSENQHRENEAHKEPFEKDSAVKYNFSFNESNGDLSNINENIAPKFRLS
MPPLSVEHPPRNASRIKSWKARTINHGRGDTRNLNDITGLGHERERDRENTHYEKKPK
LDSDSEVDIRSFRQDMDL"
gene complement(542956..543044)
/gene="SUP4"
/locus_tag="YNCJ0027C"
/db_xref="GeneID:853519"
tRNA complement(join(542956..542991,543006..543044))
/gene="SUP4"
/locus_tag="YNCJ0027C"
/product="tRNA-Tyr"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Tyrosine tRNA (tRNA-Tyr), predicted by tRNAscan-SE
analysis; can mutate to suppress ochre nonsense mutations"
/db_xref="GeneID:853519"
/db_xref="SGD:S000006782"
repeat_region 543448..543770
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007046"
gene <544062..>544712
/gene="CDC8"
/locus_tag="YJR057W"
/db_xref="GeneID:853520"
mRNA <544062..>544712
/gene="CDC8"
/locus_tag="YJR057W"
/product="bifunctional thymidylate/uridylate kinase"
/transcript_id="NM_001181715.1"
/db_xref="GeneID:853520"
CDS 544062..544712
/gene="CDC8"
/locus_tag="YJR057W"
/EC_number="2.7.4.9"
/experiment="EXISTENCE:direct assay:GO:0004550 nucleoside
diphosphate kinase activity [PMID:19540237]"
/experiment="EXISTENCE:direct assay:GO:0004798 dTMP kinase
activity [PMID:6094555|PMID:19540237|PMID:6088527]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:6094555]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:6094555]"
/experiment="EXISTENCE:direct assay:GO:0006227 dUDP
biosynthetic process [PMID:6094555]"
/experiment="EXISTENCE:direct assay:GO:0006233 dTDP
biosynthetic process
[PMID:6088527|PMID:6094555|PMID:19540237]"
/experiment="EXISTENCE:direct assay:GO:0006235 dTTP
biosynthetic process [PMID:19540237]"
/experiment="EXISTENCE:direct assay:GO:0120136 dUMP kinase
activity [PMID:6094555]"
/experiment="EXISTENCE:genetic interaction:GO:0004798 dTMP
kinase activity [PMID:6091111]"
/experiment="EXISTENCE:genetic interaction:GO:0006233 dTDP
biosynthetic process [PMID:6091111]"
/experiment="EXISTENCE:mutant phenotype:GO:0004550
nucleoside diphosphate kinase activity [PMID:19540237]"
/experiment="EXISTENCE:mutant phenotype:GO:0004798 dTMP
kinase activity [PMID:6088527|PMID:6091111|PMID:19540237]"
/experiment="EXISTENCE:mutant phenotype:GO:0006233 dTDP
biosynthetic process
[PMID:19540237|PMID:6088527|PMID:6091111]"
/experiment="EXISTENCE:mutant phenotype:GO:0006235 dTTP
biosynthetic process [PMID:19540237]"
/note="Nucleoside monophosphate and nucleoside diphosphate
kinase; functions in the de novo biosynthesis of
pyrimidine deoxyribonucleotides; thymidylate/uridylate
kinase that converts nucleoside monophosphates, dTMP and
dUMP to nucleoside diphosphates, dTDP and dUDP; nucleoside
diphosphate kinase that converts nucleoside diphosphates,
dTDP and dUDP to dTTP and dUTP; essential for mitotic and
meiotic DNA replication; homologous to S. pombe tmp1;
human homolog DTYMK can complement the cdc8 null mutant"
/codon_start=1
/product="bifunctional thymidylate/uridylate kinase"
/protein_id="NP_012591.1"
/db_xref="GeneID:853520"
/db_xref="SGD:S000003818"
/translation="MMGRGKLILIEGLDRTGKTTQCNILYKKLQPNCKLLKFPERSTR
IGGLINEYLTDDSFQLSDQAIHLLFSANRWEIVDKIKKDLLEGKNIVMDRYVYSGVAY
SAAKGTNGMDLDWCLQPDVGLLKPDLTLFLSTQDVDNNAEKSGFGDERYETVKFQEKV
KQTFMKLLDKEIRKGDESITIVDVTNKGIQEVEALIWQIVEPVLSTHIDHDKFSFF"
gene complement(<544732..>545175)
/gene="APS2"
/locus_tag="YJR058C"
/gene_synonym="YAP17"
/db_xref="GeneID:853521"
mRNA complement(<544732..>545175)
/gene="APS2"
/locus_tag="YJR058C"
/gene_synonym="YAP17"
/product="Aps2p"
/transcript_id="NM_001181716.1"
/db_xref="GeneID:853521"
CDS complement(544732..545175)
/gene="APS2"
/locus_tag="YJR058C"
/gene_synonym="YAP17"
/experiment="EXISTENCE:curator inference:GO:0006886
intracellular protein transport [PMID:10564262]"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0030122 AP-2
adaptor complex [PMID:10564262]"
/experiment="EXISTENCE:direct assay:GO:0030136
clathrin-coated vesicle [PMID:8157009]"
/experiment="EXISTENCE:physical interaction:GO:0030122
AP-2 adaptor complex [PMID:10564262]"
/note="Small subunit of the clathrin-associated adaptor
complex AP-2; AP-2 is involved in protein sorting at the
plasma membrane; related to the sigma subunit of the
mammalian plasma membrane clathrin-associated protein
(AP-2) complex"
/codon_start=1
/product="Aps2p"
/protein_id="NP_012592.1"
/db_xref="GeneID:853521"
/db_xref="SGD:S000003819"
/translation="MAVQFILCFNKQGVVRLVRWFDVHSSDPQRSQDAIAQIYRLISS
RDHKHQSNFVEFSDSTKLIYRRYAGLYFVMGVDLLDDEPIYLCHIHLFVEVLDAFFGN
VCELDIVFNFYKVYMIMDEMFIGGEIQEISKDMLLERLSILDRLD"
gene <545787..>548243
/gene="PTK2"
/locus_tag="YJR059W"
/gene_synonym="STK2"
/db_xref="GeneID:853522"
mRNA <545787..>548243
/gene="PTK2"
/locus_tag="YJR059W"
/gene_synonym="STK2"
/product="protein kinase PTK2"
/transcript_id="NM_001181717.3"
/db_xref="GeneID:853522"
CDS 545787..548243
/gene="PTK2"
/locus_tag="YJR059W"
/gene_synonym="STK2"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894|PMID:16510118]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12206772]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:12206772]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16510118]"
/experiment="EXISTENCE:genetic interaction:GO:0000082 G1/S
transition of mitotic cell cycle [PMID:12518319]"
/experiment="EXISTENCE:mutant phenotype:GO:0000296
spermine transport [PMID:8920934]"
/experiment="EXISTENCE:mutant phenotype:GO:0006873
intracellular monoatomic ion homeostasis [PMID:11003661]"
/experiment="EXISTENCE:mutant phenotype:GO:0008361
regulation of cell size [PMID:12089449]"
/experiment="EXISTENCE:mutant phenotype:GO:0015847
putrescine transport [PMID:9154797]"
/experiment="EXISTENCE:mutant phenotype:GO:0015848
spermidine transport [PMID:9154797]"
/note="Serine/threonine protein kinase; involved in
regulation of ion transport across plasma membrane;
carboxyl terminus is essential for glucose-dependent Pma1p
activation via phosphorylation of Pma1p-Ser899; enhances
spermine uptake; PTK2 has a paralog, PTK1, that arose from
the whole genome duplication"
/codon_start=1
/product="protein kinase PTK2"
/protein_id="NP_012593.3"
/db_xref="GeneID:853522"
/db_xref="SGD:S000003820"
/translation="MAGNGKDKEVDKSPSVSTLKLLGKRLFNSSSHTDNSSLLLSAEQ
LGNGRSLRKRPTSPSISGSGSGGNSPSSSAGARQRSASLHRRKNNASVGFSNGSVSSH
KSSVALQDLIKHNNNPYLNSPSDILGTGTGIASTRDRDRAVLDREKEKERARNKERNT
HHAGLPQRSNSMASHHFPNENIVYNPYGISPNHARPDTAFADTLNTNKENDLSFYMHD
GNSKIRMLPLPIANPNDFLPEDMKQYSVHLTDNFVFDTDNKPIGSGGSSEVRKVKSSY
RQKDVYALKKLNMIYHESPEKFYKRCSKEFIIAKHLSHNVHITNTFYLLKVPTTTYTT
RGWGFIMELGVKDLFQLMERTGWKNVPFNEKYCLFKQVAQGIKFCHDNGIAHRDLKPE
NVLISKEGICKLTDFGISDWYHVIPHDYTSPVKTCQGMIGSPPYTPPEVMYFDAKKHY
PEKFQKPYNPLAMDSYALGIMLITMINNIIPFIDSCNTDARFREFEVSYDNFINHQNP
HFRDKGCHKPGPGSEYSLARNFKNTDATRIAWRLADPNPATRYTMDDLFNDPFFQQIE
TCVEPNDDDLVRVPELRKSTSTNDFSENSLDAPHDQEVIHTSNPFLKKETLTSKPRSM
LEIAESPSLKQKSKVKDSAKTKTHDVGDEGGNESTKPKQQDKKENLKKDEVKNGDKDK
VIEEATTTNVDSILEKPTPTSTKVEDNLSEDDSTMKELKSMLNSTPTTPTHNGPTPLP
AKAGTQLDKRMSDLSLKSETPASTKNFSAPNVSSSSNSLRSLGSPSVSSSKKKKVIHH
HLDITNSVTNMSSVSAFISR"
gene <548759..>549814
/gene="CBF1"
/locus_tag="YJR060W"
/gene_synonym="CEP1; CPF1"
/db_xref="GeneID:853523"
mRNA <548759..>549814
/gene="CBF1"
/locus_tag="YJR060W"
/gene_synonym="CEP1; CPF1"
/product="Cbf1p"
/transcript_id="NM_001181718.1"
/db_xref="GeneID:853523"
CDS 548759..549814
/gene="CBF1"
/locus_tag="YJR060W"
/gene_synonym="CEP1; CPF1"
/experiment="EXISTENCE:direct assay:GO:0000775 chromosome,
centromeric region [PMID:9407032]"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:8665859|PMID:7891681|PMID:9894911]"
/experiment="EXISTENCE:direct assay:GO:0001227 DNA-binding
transcription repressor activity, RNA polymerase
II-specific [PMID:15302821]"
/experiment="EXISTENCE:direct assay:GO:0001228 DNA-binding
transcription activator activity, RNA polymerase
II-specific [PMID:7891681]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0006338 chromatin
remodeling [PMID:15111622]"
/experiment="EXISTENCE:direct assay:GO:0019237 centromeric
DNA binding
[PMID:2249662|PMID:2057354|PMID:9407032|PMID:11222754]"
/experiment="EXISTENCE:direct assay:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:12820973]"
/experiment="EXISTENCE:direct assay:GO:0089713
Cbf1-Met4-Met28 complex [PMID:8665859|PMID:9171357]"
/experiment="EXISTENCE:genetic interaction:GO:0007059
chromosome segregation [PMID:9584087]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:10921921|PMID:22696683|PMID:15302821]"
/experiment="EXISTENCE:mutant phenotype:GO:0006338
chromatin remodeling
[PMID:2249662|PMID:7891681|PMID:21131275]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:7891681]"
/experiment="EXISTENCE:mutant phenotype:GO:0007059
chromosome segregation
[PMID:2188087|PMID:2057354|PMID:2185892]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:20935143|PMID:17351075|PMID:7601277|PMID:7891681|PMI
D:22696683]"
/experiment="EXISTENCE:mutant phenotype:GO:0061431
cellular response to methionine [PMID:7891681]"
/experiment="EXISTENCE:mutant phenotype:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:20935143]"
/experiment="EXISTENCE:mutant phenotype:GO:1900059
positive regulation of sulfate assimilation
[PMID:7891681|PMID:7601277]"
/experiment="EXISTENCE:mutant phenotype:GO:1900060
negative regulation of ceramide biosynthetic process
[PMID:15302821]"
/experiment="EXISTENCE:mutant phenotype:GO:1900090
positive regulation of inositol biosynthetic process
[PMID:20935143|PMID:17351075]"
/experiment="EXISTENCE:physical interaction:GO:0000776
kinetochore [PMID:11070082]"
/experiment="EXISTENCE:physical interaction:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:8665859]"
/note="Basic helix-loop-helix (bHLH) protein; forms
homodimer to bind E-box consensus sequence CACGTG present
at MET gene promoters and centromere DNA element I (CDEI);
affects nucleosome positioning at this motif; associates
with other transcription factors such as Met4p and Isw1p
to mediate transcriptional activation or repression;
associates with kinetochore proteins, required for
chromosome segregation; protein abundance increases in
response to DNA replication stress"
/codon_start=1
/product="Cbf1p"
/protein_id="NP_012594.1"
/db_xref="GeneID:853523"
/db_xref="SGD:S000003821"
/translation="MNSLANNNKLSTEDEEIHSARKRGYNEEQNYSEARKKQRDQGLL
SQESNDGNIDSALLSEGATLKGTQSQYESGLTSNKDEKGSDDEDASVAEAAVAATVNY
TDLIQGQEDSSDAHTSNQTNANGEHKDSLNGERAITPSNEGVKPNTSLEGMTSSPMES
TQQSKNDMLIPLAEHDRGPEHQQDDEDNDDADIDLKKDISMQPGRRGRKPTTLATTDE
WKKQRKDSHKEVERRRRENINTAINVLSDLLPVRESSKAAILACAAEYIQKLKETDEA
NIEKWTLQKLLSEQNASQLASANEKLQEELGNAYKEIEYMKRVLRKEGIEYEDMHTHK
KQENERKSTRSDNPHEA"
gene <550511..>553318
/gene="MNN14"
/locus_tag="YJR061W"
/db_xref="GeneID:853524"
mRNA <550511..>553318
/gene="MNN14"
/locus_tag="YJR061W"
/product="Mnn14p"
/transcript_id="NM_001181719.1"
/db_xref="GeneID:853524"
CDS 550511..553318
/gene="MNN14"
/locus_tag="YJR061W"
/experiment="EXISTENCE:genetic interaction:GO:0006491
N-glycan processing [PMID:28101612]"
/note="Protein required for N-glycan
mannosylphosphorylation; non-essential gene; transcription
repressed by Rm101p; MNN14 has a paralog, MNN4, that arose
from the whole genome duplication"
/codon_start=1
/product="Mnn14p"
/protein_id="NP_012595.1"
/db_xref="GeneID:853524"
/db_xref="SGD:S000003822"
/translation="MMLSLRRFSMYVLRSLRLHFKKIIITLLTIQLLFITIFVLGGRS
SIIDGNWKSFMALFFKPLAYTNRNNNHASFDLRSKDNVAKLYEKMNFDTSGKWIDTYT
LKNNLLTVKMGPEKGQVLDSVDELRYYDNDPRLVWSVLLDHLLESDSNEYAFSWYDWA
NFDSTNKLIALRHTNISCQFVCEGAFDKNVLEMVESEVQEPLFVTNRNKYDESLWYNR
VRKVVDSNSVQQAIHDHCMNNDAYSNGTPFELPFIISEISERLRPEVYDLQAKNHLLY
SNFTPLSLTVLDSDKDAYRINLKTTDSSKSNIVQTNLLQNYIKRHRNEMVNGDLIFNH
TSMFEKFLHHGSTKKRKLDVEALDKTIYAGEYLELSPSDFQFNAKERIIELETRLRSE
GLPSHDTHYLRSLKTSVNTSPALQQKYFAEASDITDATADGHHRDRRFFSIGHNLLND
PQEFEARLNSLIRNFQKFVKANGLISWLSHGTLYGYLYDGLKFPWDVDHDLQMPIKHL
HYLSQYFNQSLILEDPREGNGRFLLDVGSAITVGVHGNGENNIDARFIDIDSGIYIDI
TGLSVSSDAAKQYMSKFVEEESSGESFSALIEDYKFDENDYFDEVDGREGLAKYTIHE
LMEWVNSHPDDFTDAEKNLVTKTYKKELAISRSDYAEKDLSPKQRYLVNEKYNLYNCR
NQHFSSLNIISPLRNTMFSGVSAFVPNRPIATLNNEYKVPAKYGLLSFQGKVYLPEFR
YWFSFADMKKFANLQLKEPKITRLESPLNDLKFSDISLLITNILKCGFHSVFASLFNS
FDSTVYRLKELEIQYDPSLSEEEKSSLLKTLRRGMSKKIKSPEKDPIIYIYERKLWEN
VEKLLNASNIYNIASQVEKEKGKEFVERSQQVYERNFDGFRLPDGGNSKTVNDLNSKG
LNLFGDNKKTSNNIFGSDQKY"
gene complement(<553476..>554849)
/gene="NTA1"
/locus_tag="YJR062C"
/gene_synonym="DEA1"
/db_xref="GeneID:853525"
mRNA complement(<553476..>554849)
/gene="NTA1"
/locus_tag="YJR062C"
/gene_synonym="DEA1"
/product="amidase"
/transcript_id="NM_001181720.3"
/db_xref="GeneID:853525"
CDS complement(553476..554849)
/gene="NTA1"
/locus_tag="YJR062C"
/gene_synonym="DEA1"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008418
protein-N-terminal asparagine amidohydrolase activity
[PMID:7744855|PMID:23218487]"
/experiment="EXISTENCE:direct assay:GO:0070773
protein-N-terminal glutamine amidohydrolase activity
[PMID:23218487]"
/experiment="EXISTENCE:mutant phenotype:GO:0008418
protein-N-terminal asparagine amidohydrolase activity
[PMID:7744855]"
/experiment="EXISTENCE:mutant phenotype:GO:0030163 protein
catabolic process [PMID:7744855]"
/experiment="EXISTENCE:mutant phenotype:GO:0031365
N-terminal protein amino acid modification [PMID:7744855]"
/experiment="EXISTENCE:mutant phenotype:GO:0070773
protein-N-terminal glutamine amidohydrolase activity
[PMID:7744855]"
/note="Amidase; removes the amide group from N-terminal
asparagine and glutamine residues to generate proteins
with N-terminal aspartate and glutamate residues that are
targets of ubiquitin-mediated degradation"
/codon_start=1
/product="amidase"
/protein_id="NP_012596.3"
/db_xref="GeneID:853525"
/db_xref="SGD:S000003823"
/translation="MLIDAIHGAKMSTKLLVSLKVLVIQLNPQIGQVDQTIKRTWSIL
DKVTKSATYVKPDIILFPEFALTGYSFHARKDILPYVTKKDEGPSFELAKSISEKFQC
YTIIGYPEDDDEQKLYNSALVVNPQGEQIFNYRKTFLYDTEMNWDCEENPEGFQTFPM
DFSKCAKLSNEDSYNRDVTLKASIGICMDLSPYKFMAPFNHFEFSSFCVDNNVELILC
PMAWLNSTSITDKQTLHNNSLLEAAKNKIAFALKEQGLPLAGSQGIYQLKIGDSQRTP
RVPSDDSTSEYKDMDEPDMSNVNYWILRFFPFLYFKSRINWFKNSSLIESILGKTRMP
LDHEYYKDGKHKEDTIDLLDSEEVIKDTVLEKTFLGTSLGQPWKFQGKNAILVLANRC
GTEDGTTIFAGSSGIYKFNGKKPKGSQDDDESSLDSLNESVELLGNLGKGLEGAILRE
VQFEVFR"
gene <555195..>555572
/gene="RPA12"
/locus_tag="YJR063W"
/gene_synonym="RRN4"
/db_xref="GeneID:853526"
mRNA <555195..>555572
/gene="RPA12"
/locus_tag="YJR063W"
/gene_synonym="RRN4"
/product="DNA-directed RNA polymerase I core subunit
RPA12"
/transcript_id="NM_001181721.1"
/db_xref="GeneID:853526"
CDS 555195..555572
/gene="RPA12"
/locus_tag="YJR063W"
/gene_synonym="RRN4"
/experiment="EXISTENCE:direct assay:GO:0003899
DNA-directed RNA polymerase activity [PMID:9837969]"
/experiment="EXISTENCE:direct assay:GO:0005736 RNA
polymerase I complex
[PMID:9837969|PMID:11486042|PMID:8417319]"
/experiment="EXISTENCE:direct assay:GO:0006360
transcription by RNA polymerase I [PMID:9837969]"
/experiment="EXISTENCE:genetic interaction:GO:0042790
nucleolar large rRNA transcription by RNA polymerase I
[PMID:9121426|PMID:1871118]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:27637775]"
/experiment="EXISTENCE:mutant phenotype:GO:0006363
termination of RNA polymerase I transcription
[PMID:18413718|PMID:15073335]"
/experiment="EXISTENCE:physical interaction:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:27637775]"
/note="RNA polymerase I subunit A12.2; contains two zinc
binding domains, and the N terminal domain is responsible
for anchoring to the RNA pol I complex; physically
interacts with transcriptional activator Msn4p, to
regulate transcription of AYR1, a gene involved in lipid
metabolism"
/codon_start=1
/product="DNA-directed RNA polymerase I core subunit
RPA12"
/protein_id="NP_012597.1"
/db_xref="GeneID:853526"
/db_xref="SGD:S000003824"
/translation="MSVVGSLIFCLDCGDLLENPNAVLGSNVECSQCKAIYPKSQFSN
LKVVTTTADDAFPSSLRAKKSVVKTSLKKNELKDGATIKEKCPQCGNEEMNYHTLQLR
SADEGATVFYTCTSCGYKFRTNN"
gene <555914..>557602
/gene="CCT5"
/locus_tag="YJR064W"
/gene_synonym="TCP5"
/db_xref="GeneID:853527"
mRNA <555914..>557602
/gene="CCT5"
/locus_tag="YJR064W"
/gene_synonym="TCP5"
/product="chaperonin-containing T-complex subunit CCT5"
/transcript_id="NM_001181722.1"
/db_xref="GeneID:853527"
CDS 555914..557602
/gene="CCT5"
/locus_tag="YJR064W"
/gene_synonym="TCP5"
/experiment="EXISTENCE:direct assay:GO:0005832
chaperonin-containing T-complex [PMID:16762366]"
/experiment="EXISTENCE:direct assay:GO:0006457 protein
folding [PMID:16762366]"
/experiment="EXISTENCE:direct assay:GO:0051082 unfolded
protein binding [PMID:16762366]"
/experiment="EXISTENCE:physical interaction:GO:0005832
chaperonin-containing T-complex [PMID:15704212]"
/note="Subunit of the cytosolic chaperonin Cct ring
complex; related to Tcp1p, required for the assembly of
actin and tubulins in vivo"
/codon_start=1
/product="chaperonin-containing T-complex subunit CCT5"
/protein_id="NP_012598.1"
/db_xref="GeneID:853527"
/db_xref="SGD:S000003825"
/translation="MAARPQQPPMEMPDLSNAIVAQDEMGRPFIIVKDQGNKKRQHGL
EAKKSHILAARSVASIIKTSLGPRGLDKILISPDGEITITNDGATILSQMELDNEIAK
LLVQLSKSQDDEIGDGTTGVVVLASALLDQALELIQKGIHPIKIANGFDEAAKLAISK
LEETCDDISASNDELFRDFLLRAAKTSLGSKIVSKDHDRFAEMAVEAVINVMDKDRKD
VDFDLIKMQGRVGGSISDSKLINGVILDKDFSHPQMPKCVLPKEGSDGVKLAILTCPF
EPPKPKTKHKLDISSVEEYQKLQTYEQDKFKEMIDDVKKAGADVVICQWGFDDEANHL
LLQNDLPAVRWVGGQELEHIAISTNGRIVPRFQDLSKDKLGTCSRIYEQEFGTTKDRM
LIIEQSKETKTVTCFVRGSNKMIVDEAERALHDSLCVVRNLVKDSRVVYGGGAAEVTM
SLAVSEEADKQRGIDQYAFRGFAQALDTIPMTLAENSGLDPIGTLSTLKSKQLKEKIS
NIGVDCLGYGSNDMKELFVVDPFIGKKQQILLATQLCRMILKIDNVIISGKDEY"
gene complement(<557809..>559158)
/gene="ARP3"
/locus_tag="YJR065C"
/gene_synonym="ACT4"
/db_xref="GeneID:853528"
mRNA complement(<557809..>559158)
/gene="ARP3"
/locus_tag="YJR065C"
/gene_synonym="ACT4"
/product="actin-related protein 3"
/transcript_id="NM_001181723.3"
/db_xref="GeneID:853528"
CDS complement(557809..559158)
/gene="ARP3"
/locus_tag="YJR065C"
/gene_synonym="ACT4"
/experiment="EXISTENCE:direct assay:GO:0005524 ATP binding
[PMID:15657399]"
/experiment="EXISTENCE:direct assay:GO:0005885 Arp2/3
protein complex [PMID:10377407]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836]"
/experiment="EXISTENCE:direct assay:GO:0030479 actin
cortical patch [PMID:9210376]"
/experiment="EXISTENCE:mutant phenotype:GO:0005524 ATP
binding [PMID:15657399]"
/experiment="EXISTENCE:mutant phenotype:GO:0034314 Arp2/3
complex-mediated actin nucleation [PMID:16862144]"
/experiment="EXISTENCE:mutant phenotype:GO:0044396 actin
cortical patch organization [PMID:10377407|PMID:15534003]"
/note="Essential component of the Arp2/3 complex; Arp2/3
is a highly conserved actin nucleation center required for
the motility and integrity of actin patches; involved in
endocytosis and membrane growth and polarity"
/codon_start=1
/product="actin-related protein 3"
/protein_id="NP_012599.3"
/db_xref="GeneID:853528"
/db_xref="SGD:S000003826"
/translation="MSYLNNPAVVMDNGTGLTKLGFAGNDSPSWVFPTAIATAAPSNT
KKSSGVGAPSAVSNEASYFGNSTSATNFNGATGGLLSNNLSGKRGTEDLDFYIGNEAL
VASQGPSYSLSYPIRHGQVENWDHMERFWENSIFKYLRTEPEDHFFLLTEPPLNPPEN
REQVAEIFFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVIDSGDGVTHVIPV
AEGYVIGSAIKNIPIAGRDITLFIQSLLRERGEADTSLRTAEKIKQEYCYVCPDIVKE
FNKFDKDPSKFAQFVVENQEKTRRKVVDIGYERFLAPEIFFNPEIASSDFLTPLPTVV
DQTIQACPIDVRKGLYNNIVLSGGSTMFKDFGRRLQRDLKSIVNNRIAQSELLSGTKS
TGVDVSVISHRKQRNAVWFGGSLLAQTAEFKGYCHTKKDYEEYGPEIVRNFSLFNMV"
gene <559416..>566828
/gene="TOR1"
/locus_tag="YJR066W"
/gene_synonym="DRR1"
/db_xref="GeneID:853529"
mRNA <559416..>566828
/gene="TOR1"
/locus_tag="YJR066W"
/gene_synonym="DRR1"
/product="phosphatidylinositol kinase-related protein
kinase TOR1"
/transcript_id="NM_001181724.1"
/db_xref="GeneID:853529"
CDS 559416..566828
/gene="TOR1"
/locus_tag="YJR066W"
/gene_synonym="DRR1"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0000139 Golgi
membrane [PMID:12719473]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane
[PMID:25046117|PMID:26928762|PMID:12719473|PMID:19748353|P
MID:18723607]"
/experiment="EXISTENCE:direct assay:GO:0004674 protein
serine/threonine kinase activity
[PMID:38127619|PMID:26582391]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:16900101]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:16900101|PMID:18723607]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:10973982]"
/experiment="EXISTENCE:direct assay:GO:0010008 endosome
membrane [PMID:12719473]"
/experiment="EXISTENCE:direct assay:GO:0018105
peptidyl-serine phosphorylation [PMID:26582391]"
/experiment="EXISTENCE:genetic interaction:GO:0006995
cellular response to nitrogen starvation [PMID:9461583]"
/experiment="EXISTENCE:genetic interaction:GO:0010507
negative regulation of autophagy [PMID:9461583]"
/experiment="EXISTENCE:genetic interaction:GO:0034599
cellular response to oxidative stress [PMID:27922823]"
/experiment="EXISTENCE:genetic interaction:GO:0034605
cellular response to heat [PMID:27922823]"
/experiment="EXISTENCE:genetic interaction:GO:1905356
regulation of snRNA pseudouridine synthesis
[PMID:27268497]"
/experiment="EXISTENCE:mutant phenotype:GO:0004672 protein
kinase activity [PMID:10329624]"
/experiment="EXISTENCE:mutant phenotype:GO:0006413
translational initiation [PMID:8741837]"
/experiment="EXISTENCE:mutant phenotype:GO:0006974 DNA
damage response [PMID:17698581]"
/experiment="EXISTENCE:mutant phenotype:GO:0031505
fungal-type cell wall organization [PMID:14736892]"
/experiment="EXISTENCE:mutant phenotype:GO:0031929 TOR
signaling [PMID:12719473|PMID:8186460]"
/experiment="EXISTENCE:mutant phenotype:GO:0031930
mitochondria-nucleus signaling pathway [PMID:11997479]"
/experiment="EXISTENCE:mutant phenotype:GO:0034976
response to endoplasmic reticulum stress [PMID:31144305]"
/experiment="EXISTENCE:mutant phenotype:GO:0042254
ribosome biogenesis [PMID:10198052]"
/experiment="EXISTENCE:mutant phenotype:GO:0042790
nucleolar large rRNA transcription by RNA polymerase I
[PMID:16900101]"
/experiment="EXISTENCE:mutant phenotype:GO:0051321 meiotic
cell cycle [PMID:9096347]"
/experiment="EXISTENCE:mutant phenotype:GO:0051726
regulation of cell cycle [PMID:8741837]"
/experiment="EXISTENCE:mutant phenotype:GO:0090153
regulation of sphingolipid biosynthetic process
[PMID:23363605]"
/experiment="EXISTENCE:physical interaction:GO:0031931
TORC1 complex [PMID:12408816]"
/note="PIK-related protein kinase and rapamycin target;
subunit of TORC1, a complex that controls growth in
response to nutrients by regulating translation,
transcription, ribosome biogenesis, nutrient transport and
autophagy; involved in meiosis; indole-3-acetic acid, an
ATP-competitive TORC1 inhibitor, accumulates upon entry
into stationary phase; malonyl-CoA is a direct
ATP-competitive inhibitor of TORC1; TOR1 has a paralog,
TOR2, that arose from the whole genome duplication"
/codon_start=1
/product="phosphatidylinositol kinase-related protein
kinase TOR1"
/protein_id="NP_012600.1"
/db_xref="GeneID:853529"
/db_xref="SGD:S000003827"
/translation="MEPHEEQIWKSKLLKAANNDMDMDRNVPLAPNLNVNMNMKMNAS
RNGDEFGLTSSRFDGVVIGSNGDVNFKPILEKIFRELTSDYKEERKLASISLFDLLVS
LEHELSIEEFQAVSNDINNKILELVHTKKTSTRVGAVLSIDTLISFYAYTERLPNETS
RLAGYLRGLIPSNDVEVMRLAAKTLGKLAVPGGTYTSDFVEFEIKSCLEWLTASTEKN
SFSSSKPDHAKHAALLIITALAENCPYLLYQYLNSILDNIWRALRDPHLVIRIDASIT
LAKCLSTLRNRDPQLTSQWVQRLATSCEYGFQVNTLECIHASLLVYKEILFLKDPFLN
QVFDQMCLNCIAYENHKAKMIREKIYQIVPLLASFNPQLFAGKYLHQIMDNYLEILTN
APANKIPHLKDDKPQILISIGDIAYEVGPDIAPYVKQILDYIEHDLQTKFKFRKKFEN
EIFYCIGRLAVPLGPVLGKLLNRNILDLMFKCPLSDYMQETFQILTERIPSLGPKIND
ELLNLVCSTLSGTPFIQPGSPMEIPSFSRERAREWRNKNILQKTGESNDDNNDIKIII
QAFRMLKNIKSRFSLVEFVRIVALSYIEHTDPRVRKLAALTSCEIYVKDNICKQTSLH
SLNTVSEVLSKLLAITIADPLQDIRLEVLKNLNPCFDPQLAQPDNLRLLFTALHDESF
NIQSVAMELVGRLSSVNPAYVIPSIRKILLELLTKLKFSTSSREKEETASLLCTLIRS
SKDVAKPYIEPLLNVLLPKFQDTSSTVASTALRTIGELSVVGGEDMKIYLKDLFPLII
KTFQDQSNSFKREAALKALGQLAASSGYVIDPLLDYPELLGILVNILKTENSQNIRRQ
TVTLIGILGAIDPYRQKEREVTSTTDISTEQNAPPIDIALLMQGMSPSNDEYYTTVVI
HCLLKILKDPSLSSYHTAVIQAIMHIFQTLGLKCVSFLDQIIPTILDVMRTCSQSLLE
FYFQQLCSLIIIVRQHIRPHVDSIFQAIKDFSSVAKLQITLVSVIEAISKALEGEFKR
LVPLTLTLFLVILENDKSSDKVLSRRVLRLLESFGPNLEGYSHLITPKIVQMAEFTSG
NLQRSAIITIGKLAKDVDLFEMSSRIVHSLLRVLSSTTSDELSKVIMNTLSLLLIQMG
TSFAIFIPVINEVLMKKHIQHTIYDDLTNRILNNDVLPTKILEANTTDYKPAEQMEAA
DAGVAKLPINQSVLKSAWNSSQQRTKEDWQEWSKRLSIQLLKESPSHALRACSNLASM
YYPLAKELFNTAFACVWTELYSQYQEDLIGSLCIALSSPLNPPEIHQTLLNLVEFMEH
DDKALPIPTQSLGEYAERCHAYAKALHYKEIKFIKEPENSTIESLISINNQLNQTDAA
IGILKHAQQHHSLQLKETWFEKLERWEDALHAYNEREKAGDTSVSVTLGKMRSLHALG
EWEQLSQLAARKWKVSKLQTKKLIAPLAAGAAWGLGEWDMLEQYISVMKPKSPDKEFF
DAILYLHKNDYDNASKHILNARDLLVTEISALINESYNRAYSVIVRTQIITEFEEIIK
YKQLPPNSEKKLHYQNLWTKRLLGCQKNVDLWQRVLRVRSLVIKPKQDLQIWIKFANL
CRKSGRMRLANKALNMLLEGGNDPSLPNTFKAPPPVVYAQLKYIWATGAYKEALNHLI
GFTSRLAHDLGLDPNNMIAQSVKLSSASTAPYVEEYTKLLARCFLKQGEWRIATQPNW
RNTNPDAILGSYLLATHFDKNWYKAWHNWALANFEVISMVQEETKLNGGKNDDDDDTA
VNNDNVRIDGSILGSGSLTINGNRYPLELIQRHVVPAIKGFFHSISLLETSCLQDTLR
LLTLLFNFGGIKEVSQAMYEGFNLMKIENWLEVLPQLISRIHQPDPTVSNSLLSLLSD
LGKAHPQALVYPLTVAIKSESVSRQKAALSIIEKIRIHSPVLVNQAELVSHELIRVAV
LWHELWYEGLEDASRQFFVEHNIEKMFSTLEPLHKHLGNEPQTLSEVSFQKSFGRDLN
DAYEWLNNYKKSKDINNLNQAWDIYYNVFRKITRQIPQLQTLDLQHVSPQLLATHDLE
LAVPGTYFPGKPTIRIAKFEPLFSVISSKQRPRKFSIKGSDGKDYKYVLKGHEDIRQD
SLVMQLFGLVNTLLKNDSECFKRHLDIQQYPAIPLSPKSGLLGWVPNSDTFHVLIREH
RDAKKIPLNIEHWVMLQMAPDYENLTLLQKIEVFTYALDNTKGQDLYKILWLKSRSSE
TWLERRTTYTRSLAVMSMTGYILGLGDRHPSNLMLDRITGKVIHIDFGDCFEAAILRE
KYPEKVPFRLTRMLTYAMEVSGIEGSFRITCENVMRVLRDNKESLMAILEAFALDPLI
HWGFDLPPQKLTEQTGIPLPLINPSELLRKGAITVEEAANMEAEQQNETKNARAMLVL
RRITDKLTGNDIKRFNELDVPEQVDKLIQQATSIERLCQHYIGWCPFW"
gene complement(<567019..>567444)
/gene="YAE1"
/locus_tag="YJR067C"
/db_xref="GeneID:853530"
mRNA complement(<567019..>567444)
/gene="YAE1"
/locus_tag="YJR067C"
/product="Yae1p"
/transcript_id="NM_001181725.1"
/db_xref="GeneID:853530"
CDS complement(567019..567444)
/gene="YAE1"
/locus_tag="YJR067C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0030674
protein-macromolecule adaptor activity [PMID:26182403]"
/experiment="EXISTENCE:direct assay:GO:0062092 Yae1-Lto1
complex [PMID:26182403]"
/experiment="EXISTENCE:mutant phenotype:GO:0051604 protein
maturation [PMID:26182403]"
/note="Substrate-specific adaptor protein involved in
apo-Rli1p maturation; subunit of the Yae1-Lto1 complex,
recruiting apo-Rli1p to the CIA targeting complex,
facilitating the insertion of an Fe/S cluster by the Fe-S
biosynthetic machinery; deca-GX3 motif crucial for complex
formation; essential for aerobic but not anaerobic growth;
homolog of human YAE1D1, which is overexpressed in oral
cancers; co-expression of YAE1D1 and LTO1 homolog ORAOV1
restores growth and Rli1p maturation after YAE1 depletion"
/codon_start=1
/product="Yae1p"
/protein_id="NP_012601.1"
/db_xref="GeneID:853530"
/db_xref="SGD:S000003828"
/translation="MSNTWDDVWASDSDVETERSPDLVKLRENHSKRGYLDGIVSSKE
EKLQEGFNDGFPTGAKLGKQVGIIMGILLGLRTRFGDEDEDLSKAYIDAQKELRINKV
LSKSIFDPNFDLQEKHPLITKWTDIANTYCEKYHVPSIQ"
gene <567643..>568704
/gene="RFC2"
/locus_tag="YJR068W"
/db_xref="GeneID:853531"
mRNA <567643..>568704
/gene="RFC2"
/locus_tag="YJR068W"
/product="replication factor C subunit 2"
/transcript_id="NM_001181726.3"
/db_xref="GeneID:853531"
CDS 567643..568704
/gene="RFC2"
/locus_tag="YJR068W"
/experiment="EXISTENCE:direct assay:GO:0003689 DNA clamp
loader activity [PMID:12604797|PMID:8995429]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:23613772]"
/experiment="EXISTENCE:direct assay:GO:0005663 DNA
replication factor C complex [PMID:8202350]"
/experiment="EXISTENCE:direct assay:GO:0006272 leading
strand elongation [PMID:1346062]"
/experiment="EXISTENCE:direct assay:GO:0031389 Rad17
RFC-like complex [PMID:12604797|PMID:10660302]"
/experiment="EXISTENCE:mutant phenotype:GO:0000076 DNA
replication checkpoint signaling [PMID:9671499]"
/experiment="EXISTENCE:physical interaction:GO:0007062
sister chromatid cohesion [PMID:11389843]"
/experiment="EXISTENCE:physical interaction:GO:0031389
Rad17 RFC-like complex [PMID:10660302]"
/experiment="EXISTENCE:physical interaction:GO:0031390
Ctf18 RFC-like complex [PMID:11389843]"
/experiment="EXISTENCE:physical interaction:GO:0031391
Elg1 RFC-like complex [PMID:12912927]"
/note="Subunit of heteropentameric Replication factor C
(RF-C); RF-C is a DNA binding protein and ATPase that acts
as a clamp loader of the proliferating cell nuclear
antigen (PCNA) processivity factor for DNA polymerases
delta and epsilon"
/codon_start=1
/product="replication factor C subunit 2"
/protein_id="NP_012602.3"
/db_xref="GeneID:853531"
/db_xref="SGD:S000003829"
/translation="MFEGFGPNKKRKISKLAAEQSLAQQPWVEKYRPKNLDEVTAQDH
AVTVLKKTLKSANLPHMLFYGPPGTGKTSTILALTKELYGPDLMKSRILELNASDERG
ISIVREKVKNFARLTVSKPSKHDLENYPCPPYKIIILDEADSMTADAQSALRRTMETY
SGVTRFCLICNYVTRIIDPLASRCSKFRFKALDASNAIDRLRFISEQENVKCDDGVLE
RILDISAGDLRRGITLLQSASKGAQYLGDGKNITSTQVEELAGVVPHDILIEIVEKVK
SGDFDEIKKYVNTFMKSGWSAASVVNQLHEYYITNDNFDTNFKNQISWLLFTTDSRLN
NGTNEHIQLLNLLVKISQL"
gene complement(<568806..>569399)
/gene="HAM1"
/locus_tag="YJR069C"
/db_xref="GeneID:853532"
mRNA complement(<568806..>569399)
/gene="HAM1"
/locus_tag="YJR069C"
/product="nucleoside triphosphate pyrophosphohydrolase
HAM1"
/transcript_id="NM_001181727.1"
/db_xref="GeneID:853532"
CDS complement(568806..569399)
/gene="HAM1"
/locus_tag="YJR069C"
/EC_number="3.6.1.66"
/experiment="EXISTENCE:direct assay:GO:0004170 dUTP
diphosphatase activity [PMID:17899088]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008828 dATP
diphosphatase activity [PMID:22531138]"
/experiment="EXISTENCE:direct assay:GO:0009213 pyrimidine
deoxyribonucleoside triphosphate catabolic process
[PMID:17899088|PMID:22531138]"
/experiment="EXISTENCE:direct assay:GO:0009217 purine
deoxyribonucleoside triphosphate catabolic process
[PMID:17899088|PMID:22531138|PMID:17090528]"
/experiment="EXISTENCE:direct assay:GO:0035870 dITP
diphosphatase activity [PMID:22531138|PMID:17090528]"
/experiment="EXISTENCE:direct assay:GO:0036217 dGTP
diphosphatase activity [PMID:22531138|PMID:17090528]"
/experiment="EXISTENCE:direct assay:GO:0036218 dTTP
diphosphatase activity [PMID:22531138]"
/experiment="EXISTENCE:direct assay:GO:0036219 GTP
diphosphatase activity [PMID:22531138]"
/experiment="EXISTENCE:direct assay:GO:0036220 ITP
diphosphatase activity [PMID:17899088|PMID:22531138]"
/experiment="EXISTENCE:direct assay:GO:0036221 UTP
diphosphatase activity [PMID:17899088]"
/experiment="EXISTENCE:direct assay:GO:0036222 XTP
diphosphatase activity [PMID:22531138]"
/experiment="EXISTENCE:direct assay:GO:0047840 dCTP
diphosphatase activity [PMID:22531138]"
/experiment="EXISTENCE:mutant phenotype:GO:0009213
pyrimidine deoxyribonucleoside triphosphate catabolic
process [PMID:17899088]"
/experiment="EXISTENCE:mutant phenotype:GO:0009217 purine
deoxyribonucleoside triphosphate catabolic process
[PMID:22308425]"
/note="Nucleoside triphosphate pyrophosphohydrolase;
active against various substrates including ITP, dITP and
XTP; mediates exclusion of non canonical purines,
pyrimidines from dNTP pools; functions with YJL055W to
mediate resistance to 5-FU; specifically reduces the
incorporation of 5-FU into RNA without affecting uptake or
incorporation of uracil into RNA; protein abundance
increases in response to DNA replication stress; yeast
HAM1 can complement knockdown of human homolog ITPA"
/codon_start=1
/product="nucleoside triphosphate pyrophosphohydrolase
HAM1"
/protein_id="NP_012603.1"
/db_xref="GeneID:853532"
/db_xref="SGD:S000003830"
/translation="MSNNEIVFVTGNANKLKEVQSILTQEVDNNNKTIHLINEALDLE
ELQDTDLNAIALAKGKQAVAALGKGKPVFVEDTALRFDEFNGLPGAYIKWFLKSMGLE
KIVKMLEPFENKNAEAVTTICFADSRGEYHFFQGITRGKIVPSRGPTTFGWDSIFEPF
DSHGLTYAEMSKDAKNAISHRGKAFAQFKEYLYQNDF"
gene complement(<569621..>570598)
/gene="LIA1"
/locus_tag="YJR070C"
/gene_synonym="MMD1"
/db_xref="GeneID:853534"
mRNA complement(<569621..>570598)
/gene="LIA1"
/locus_tag="YJR070C"
/gene_synonym="MMD1"
/product="deoxyhypusine monooxygenase"
/transcript_id="NM_001181728.1"
/db_xref="GeneID:853534"
CDS complement(569621..570598)
/gene="LIA1"
/locus_tag="YJR070C"
/gene_synonym="MMD1"
/EC_number="1.14.99.29"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008612
peptidyl-lysine modification to peptidyl-hypusine
[PMID:16371467]"
/experiment="EXISTENCE:direct assay:GO:0019135
deoxyhypusine monooxygenase activity [PMID:16371467]"
/experiment="EXISTENCE:mutant phenotype:GO:0000226
microtubule cytoskeleton organization [PMID:14767070]"
/experiment="EXISTENCE:mutant phenotype:GO:0008612
peptidyl-lysine modification to peptidyl-hypusine
[PMID:16371467]"
/experiment="EXISTENCE:mutant phenotype:GO:0019135
deoxyhypusine monooxygenase activity [PMID:16371467]"
/note="Deoxyhypusine hydroxylase; HEAT-repeat containing
metalloenzyme that catalyzes hypusine formation; binds to
and is required for the modification of Hyp2p (eIF5A);
complements S. pombe mmd1 mutants defective in
mitochondrial positioning; protein abundance increases in
response to DNA replication stress"
/codon_start=1
/product="deoxyhypusine monooxygenase"
/protein_id="NP_012604.1"
/db_xref="GeneID:853534"
/db_xref="SGD:S000003831"
/translation="MSTNFEKHFQENVDECTLEQLRDILVNKSGKTVLANRFRALFNL
KTVAEEFATKPEEAKKAIEYIAESFVNDKSELLKHEVAYVLGQTKNLDAAPTLRHVML
DQNQEPMVRHEAAEALGALGDKDSLDDLNKAAKEDPHVAVRETCELAINRINWTHGGA
KDKENLQQSLYSSIDPAPPLPLEKDATIPELQALLNDPKQPLFQRYRAMFRLRDIGTD
EAILALATGFSAESSLFKHEIAYVFGQIGSPAAVPSLIEVLGRKEEAPMVRHEAAEAL
GAIASPEVVDVLKSYLNDEVDVVRESCIVALDMYDYENSNELEYAPTAN"
gene complement(<570967..>572124)
/gene="NPA3"
/locus_tag="YJR072C"
/gene_synonym="EPA1; GPN1"
/db_xref="GeneID:853535"
mRNA complement(<570967..>572124)
/gene="NPA3"
/locus_tag="YJR072C"
/gene_synonym="EPA1; GPN1"
/product="GTPase NPA3"
/transcript_id="NM_001181730.3"
/db_xref="GeneID:853535"
CDS complement(570967..572124)
/gene="NPA3"
/locus_tag="YJR072C"
/gene_synonym="EPA1; GPN1"
/experiment="EXISTENCE:direct assay:GO:0003924 GTPase
activity [PMID:21844196]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0006913
nucleocytoplasmic transport [PMID:21844196]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:15082539]"
/experiment="EXISTENCE:mutant phenotype:GO:0006606 protein
import into nucleus [PMID:21844196]"
/experiment="EXISTENCE:mutant phenotype:GO:0007064 mitotic
sister chromatid cohesion [PMID:21532343]"
/note="Member of the conserved GPN-loop GTPase family; has
a role in transport of RNA polymerase II to the nucleus;
exhibits GTP-dependent binding to PolII; has ATPase
activity; involved in sister chromatid cohesion;
phosphorylated by the Pcl1p-Pho85p kinase complex; human
homolog XAB1 interacts with human RNA polymerase II;
protein abundance increases in response to DNA replication
stress"
/codon_start=1
/product="GTPase NPA3"
/protein_id="NP_012606.3"
/db_xref="GeneID:853535"
/db_xref="SGD:S000003833"
/translation="MSLSTIICIGMAGSGKTTFMQRLNSHLRAEKTPPYVINLDPAVL
RVPYGANIDIRDSIKYKKVMENYQLGPNGAIVTSLNLFSTKIDQVIRLVEQKKDKFQN
CIIDTPGQIECFVWSASGAIITESFASSFPTVIAYIVDTPRNSSPTTFMSNMLYACSI
LYKTKLPMIVVFNKTDVCKADFAKEWMTDFESFQAAIKEDQDLNGDNGLGSGYMSSLV
NSMSLMLEEFYSQLDVVGVSSFTGDGFDEFMQCVDKKVDEYDQYYKQEREKALNLKKK
KEEMRKQKSLNGLMKDLGLNEKSSAAASDNDSIDAISDLEEDANDGLVDRDEDEGVER
EYTFPGEERTKGEVNENSAPDLQRRYQEAMQQVGKTASSETAENIAKYIRN"
gene complement(<572315..>572935)
/gene="OPI3"
/locus_tag="YJR073C"
/gene_synonym="PEM2"
/db_xref="GeneID:853536"
mRNA complement(<572315..>572935)
/gene="OPI3"
/locus_tag="YJR073C"
/gene_synonym="PEM2"
/product="bifunctional phosphatidyl-N-methylethanolamine
N-methyltransferase/phosphatidyl-N-dimethylethanolamine
N-methyltransferase"
/transcript_id="NM_001181731.1"
/db_xref="GeneID:853536"
CDS complement(572315..572935)
/gene="OPI3"
/locus_tag="YJR073C"
/gene_synonym="PEM2"
/EC_number="2.1.1.17"
/EC_number="2.1.1.71"
/experiment="EXISTENCE:direct assay:GO:0000773
phosphatidyl-N-methylethanolamine N-methyltransferase
activity [PMID:2445736]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0006656
phosphatidylcholine biosynthetic process [PMID:2445736]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0000773
phosphatidyl-N-methylethanolamine N-methyltransferase
activity [PMID:2445736]"
/experiment="EXISTENCE:genetic interaction:GO:0006656
phosphatidylcholine biosynthetic process [PMID:2445736]"
/experiment="EXISTENCE:mutant phenotype:GO:0000773
phosphatidyl-N-methylethanolamine N-methyltransferase
activity [PMID:2684666]"
/experiment="EXISTENCE:mutant phenotype:GO:0006656
phosphatidylcholine biosynthetic process
[PMID:6337128|PMID:23000174]"
/note="Methylene-fatty-acyl-phospholipid synthase;
catalyzes the last two steps in phosphatidylcholine
biosynthesis; also known as phospholipid
methyltransferase"
/codon_start=1
/product="bifunctional phosphatidyl-N-methylethanolamine
N-methyltransferase/phosphatidyl-N-dimethylethanolamine
N-methyltransferase"
/protein_id="NP_012607.1"
/db_xref="GeneID:853536"
/db_xref="SGD:S000003834"
/translation="MKESVQEIIQQLIHSVDLQSSKFQLAIVCTMFNPIFWNIVARME
YHKHSLTKMCGGARKGCYMLAATIFSLGIVRDMVYESALREQPTCSLITGENWTKLGV
ALFGLGQVLVLSSMYKLGITGTYLGDYFGILMDERVTGFPFNVSNNPMYQGSTLSFLG
IALYKGKPAGLVVSAVVYFMYKIALRWEEPFTAMIYANRDKAKKNM"
gene <573095..>573751
/gene="MOG1"
/locus_tag="YJR074W"
/db_xref="GeneID:853537"
mRNA <573095..>573751
/gene="MOG1"
/locus_tag="YJR074W"
/product="Ran GTPase-binding protein MOG1"
/transcript_id="NM_001181732.1"
/db_xref="GeneID:853537"
CDS 573095..573751
/gene="MOG1"
/locus_tag="YJR074W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9860978]"
/experiment="EXISTENCE:direct assay:GO:0031267 small
GTPase binding [PMID:11509570|PMID:9860978]"
/experiment="EXISTENCE:mutant phenotype:GO:0006606 protein
import into nucleus [PMID:11024003|PMID:9860978]"
/note="Conserved nuclear protein that interacts with
GTP-Gsp1p; stimulates nucleotide release from Gsp1p;
involved in nuclear protein import; nucleotide release is
inhibited by Yrb1p"
/codon_start=1
/product="Ran GTPase-binding protein MOG1"
/protein_id="NP_012608.1"
/db_xref="GeneID:853537"
/db_xref="SGD:S000003835"
/translation="MKIEKASHISQPVQLSTCTLIDTYPGHQGSMNNKEVELYGGAIT
TVVPPGFIDASTLREVPDTQEVYVNSRRDEEEFEDGLATNESIIVDLLETVDKSDLKE
AWQFHVEDLTELNGTTKWEALQEDTVQQGTKFTGLVMEVANKWGKPDLAQTVVIGVAL
IRLTQFDTDVVISINVPLTKEEASQASNKELPARCHAVYQLLQEMVRKFHVVDTSLFA
"
gene <573981..>575171
/gene="HOC1"
/locus_tag="YJR075W"
/db_xref="GeneID:853538"
mRNA <573981..>575171
/gene="HOC1"
/locus_tag="YJR075W"
/product="alpha-1,6-mannosyltransferase"
/transcript_id="NM_001181733.3"
/db_xref="GeneID:853538"
CDS 573981..575171
/gene="HOC1"
/locus_tag="YJR075W"
/experiment="EXISTENCE:direct assay:GO:0000009
alpha-1,6-mannosyltransferase activity [PMID:10037752]"
/experiment="EXISTENCE:direct assay:GO:0000136 mannan
polymerase complex [PMID:10037752]"
/experiment="EXISTENCE:physical interaction:GO:0000136
mannan polymerase complex [PMID:9430634]"
/note="Alpha-1,6-mannosyltransferase; involved in cell
wall mannan biosynthesis; subunit of a Golgi-localized
complex that also contains Anp1p, Mnn9p, Mnn11p, and
Mnn10p; identified as a suppressor of a cell lysis
sensitive pkc1-371 allele"
/codon_start=1
/product="alpha-1,6-mannosyltransferase"
/protein_id="NP_012609.3"
/db_xref="GeneID:853538"
/db_xref="SGD:S000003836"
/translation="MAKTTKRASSFRRLMIFAIIALISLAFGVRYLFHNSNATDLQKI
LQNLPKEISQSINSANNIQSSDSDLVQHFESLAQEIRHQQEVQAKQFDKQRKILEKKI
QDLKQTPPEATLRERIAMTFPYDSHVKFPAFIWQTWSNDEGPERVQDIKGMWESKNPG
FAHEVLNHDVINALVHHYFYSIPEILETYEALPSIILKIDFFKYLILLVHGGVYADID
TFPVQPIPNWIPEELSPSDIGLIVGVEEDAQRADWRTKYIRRLQFGTWIIQAKPGHPV
LREIISRIIETTLQRKRDDQLNVNLRNDLNIMSWTGSGLWTDTIFTYFNDFMRSGVRE
KVTWKLFHNLNQPKLLSDVLVFPKFSFNCPNQIDNDDPHKKFYFITHLASQFWKNTPK
VEQK"
gene complement(<575354..>576601)
/gene="CDC11"
/locus_tag="YJR076C"
/gene_synonym="PSL9"
/db_xref="GeneID:853539"
mRNA complement(<575354..>576601)
/gene="CDC11"
/locus_tag="YJR076C"
/gene_synonym="PSL9"
/product="septin CDC11"
/transcript_id="NM_001181734.1"
/db_xref="GeneID:853539"
CDS complement(575354..576601)
/gene="CDC11"
/locus_tag="YJR076C"
/gene_synonym="PSL9"
/experiment="EXISTENCE:direct assay:GO:0000144 cellular
bud neck septin ring [PMID:1934633]"
/experiment="EXISTENCE:direct assay:GO:0001400 mating
projection base [PMID:8791410]"
/experiment="EXISTENCE:direct assay:GO:0001934 positive
regulation of protein phosphorylation [PMID:14527412]"
/experiment="EXISTENCE:direct assay:GO:0005200 structural
constituent of cytoskeleton
[PMID:22144691|PMID:29321251|PMID:18550837]"
/experiment="EXISTENCE:direct assay:GO:0005525 GTP binding
[PMID:12665577]"
/experiment="EXISTENCE:direct assay:GO:0005619 ascospore
wall [PMID:8636217]"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:18826657|PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0005876 spindle
microtubule [PMID:18826657]"
/experiment="EXISTENCE:direct assay:GO:0005881 cytoplasmic
microtubule [PMID:18826657]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0010314
phosphatidylinositol-5-phosphate binding [PMID:12665577]"
/experiment="EXISTENCE:direct assay:GO:0031105 septin
complex [PMID:18550837]"
/experiment="EXISTENCE:direct assay:GO:0032160 septin
filament array [PMID:15282341]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807]"
/experiment="EXISTENCE:direct assay:GO:0070273
phosphatidylinositol-4-phosphate binding [PMID:12665577]"
/experiment="EXISTENCE:direct assay:GO:0072687 meiotic
spindle [PMID:18826657]"
/experiment="EXISTENCE:genetic interaction:GO:0097271
protein localization to bud neck [PMID:25971666]"
/experiment="EXISTENCE:mutant phenotype:GO:0000281 mitotic
cytokinesis [PMID:4950437]"
/experiment="EXISTENCE:mutant phenotype:GO:0010314
phosphatidylinositol-5-phosphate binding [PMID:12665577]"
/experiment="EXISTENCE:mutant phenotype:GO:0070273
phosphatidylinositol-4-phosphate binding [PMID:12665577]"
/experiment="EXISTENCE:physical interaction:GO:0031105
septin complex [PMID:9813094]"
/note="Component of the septin ring that is required for
cytokinesis; present at the ends of rod-like septin
hetero-oligomers; C-terminal extension is important for
recruitment of Bni5p to the mother-bud neck, which in turn
is required for Myo1p recruitment and cytokinesis; septin
rings at the mother-bud neck act as scaffolds for
recruiting cell division factors and as barriers to
prevent diffusion of specific proteins between mother and
daughter cells"
/codon_start=1
/product="septin CDC11"
/protein_id="NP_012610.1"
/db_xref="GeneID:853539"
/db_xref="SGD:S000003837"
/translation="MSGIIDASSALRKRKHLKRGITFTVMIVGQSGSGRSTFINTLCG
QQVVDTSTTILLPTDTSTEIDLQLREETVELEDDEGVKIQLNIIDTPGFGDSLDNSPS
FEIISDYIRHQYDEILLEESRVRRNPRFKDGRVHCCLYLINPTGHGLKEIDVEFIRQL
GSLVNIIPVISKSDSLTRDELKLNKKLIMEDIDRWNLPIYNFPFDEDEISDEDYETNM
YLRTLLPFAIIGSNEVYEMGGDVGTIRGRKYPWGILDVEDSSISDFVILRNALLISHL
HDLKNYTHEILYERYRTEALSGESVAAESIRPNLTKLNGSSSSSTTTRRNTNPFKQSN
NINNDVLNPASDMHGQSTGENNETYMTREEQIRLEEERLKAFEERVQQELLLKRQELL
QREKELREIEARLEKEAKIKQEE"
gene complement(<577255..>578190)
/gene="MIR1"
/locus_tag="YJR077C"
/db_xref="GeneID:853540"
mRNA complement(<577255..>578190)
/gene="MIR1"
/locus_tag="YJR077C"
/product="Mir1p"
/transcript_id="NM_001181735.1"
/db_xref="GeneID:853540"
CDS complement(577255..578190)
/gene="MIR1"
/locus_tag="YJR077C"
/experiment="EXISTENCE:direct assay:GO:0005315 phosphate
transmembrane transporter activity [PMID:9099701]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:24769239|PMID:14576278|PMID:16823961|PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:8245029]"
/experiment="EXISTENCE:direct assay:GO:0035435 phosphate
ion transmembrane transport [PMID:9099701]"
/experiment="EXISTENCE:physical interaction:GO:0005739
mitochondrion [PMID:16962558]"
/note="Mitochondrial phosphate carrier; imports inorganic
phosphate into mitochondria; functionally redundant with
Pic2p but more abundant than Pic2p under normal
conditions; phosphorylated"
/codon_start=1
/product="Mir1p"
/protein_id="NP_012611.1"
/db_xref="GeneID:853540"
/db_xref="SGD:S000003838"
/translation="MSVSAAPAIPQYSVSDYMKFALAGAIGCGSTHSSMVPIDVVKTR
IQLEPTVYNKGMVGSFKQIIAGEGAGALLTGFGPTLLGYSIQGAFKFGGYEVFKKFFI
DNLGYDTASRYKNSVYMGSAAMAEFLADIALCPLEATRIRLVSQPQFANGLVGGFSRI
LKEEGIGSFYSGFTPILFKQIPYNIAKFLVFERASEFYYGFAGPKEKLSSTSTTLLNL
LSGLTAGLAAAIVSQPADTLLSKVNKTKKAPGQSTVGLLAQLAKQLGFFGSFAGLPTR
LVMVGTLTSLQFGIYGSLKSTLGCPPTIEIGGGGH"
gene <578860..>580221
/gene="BNA2"
/locus_tag="YJR078W"
/db_xref="GeneID:853541"
mRNA <578860..>580221
/gene="BNA2"
/locus_tag="YJR078W"
/product="dioxygenase BNA2"
/transcript_id="NM_001181736.3"
/db_xref="GeneID:853541"
CDS 578860..580221
/gene="BNA2"
/locus_tag="YJR078W"
/EC_number="1.13.11.52"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0033754 indoleamine
2,3-dioxygenase activity [PMID:21170645]"
/experiment="EXISTENCE:genetic interaction:GO:0034354 'de
novo' NAD+ biosynthetic process from L-tryptophan
[PMID:12062417]"
/note="Tryptophan 2,3-dioxygenase or indoleamine
2,3-dioxygenase; required for de novo biosynthesis of NAD+
from tryptophan via kynurenine; up-regulated by telomere
uncapping, and interacts genetically with capping gene
CDC13; overexpression reduces lipid droplet accumulation
by skewing glycolytic flux towards biosynthesis of
shikimate, aromatic amino acids, and NAD+ during aging and
extends lifespan; regulated by Hst1p and Aftp"
/codon_start=1
/product="dioxygenase BNA2"
/protein_id="NP_012612.3"
/db_xref="GeneID:853541"
/db_xref="SGD:S000003839"
/translation="MNNTSITGPQVLHRTKMRPLPVLEKYCISPHHGFLDDRLPLTRL
SSKKYMKWEEIVADLPSLLQEDNKVRSVIDGLDVLDLDETILGDVRELRRAYSILGFM
AHAYIWASGTPRDVLPECIARPLLETAHILGVPPLATYSSLVLWNFKVTDECKKTETG
CLDLENITTINTFTGTVDESWFYLVSVRFEKIGSACLNHGLQILRAIRSGDKGDANVI
DGLEGLAATIERLSKALMEMELKCEPNVFYFKIRPFLAGWTNMSHMGLPQGVRYGAEG
QYRIFSGGSNAQSSLIQTLDILLGVKHTANAAHSSQGDSKINYLDEMKKYMPREHREF
LYHLESVCNIREYVSRNASNRALQEAYGRCISMLKIFRDNHIQIVTKYIILPSNSKQH
GSNKPNVLSPIEPNTKASGCLGHKVASSKTIGTGGTRLMPFLKQCRDETVATADIKNE
DKN"
gene <580205..>581239
/locus_tag="YJR079W"
/db_xref="GeneID:853542"
mRNA join(<580205..580347,581053..>581239)
/locus_tag="YJR079W"
/product="uncharacterized protein"
/transcript_id="NM_001181737.1"
/db_xref="GeneID:853542"
CDS join(580205..580347,581053..581239)
/locus_tag="YJR079W"
/note="hypothetical protein; mutation results in impaired
mitochondrial respiration"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_012613.1"
/db_xref="GeneID:853542"
/db_xref="SGD:S000003840"
/translation="MKIKIDIIHFINAPPFFFFFVDAEASQLKAFQLFLLGHIFYTYI
HTYICDFDEFETKDLAEGKIGDLISRLEFCSNAIIESLPNTFQSFVPVKFSTDKLLEE
SKGLLDV"
gene complement(<580432..>581616)
/gene="AIM24"
/locus_tag="YJR080C"
/gene_synonym="FMP26"
/db_xref="GeneID:853543"
mRNA complement(<580432..>581616)
/gene="AIM24"
/locus_tag="YJR080C"
/gene_synonym="FMP26"
/product="Aim24p"
/transcript_id="NM_001181738.1"
/db_xref="GeneID:853543"
CDS complement(580432..581616)
/gene="AIM24"
/locus_tag="YJR080C"
/gene_synonym="FMP26"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:24769239|PMID:16823961|PMID:14576278|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:24714493]"
/experiment="EXISTENCE:mutant phenotype:GO:0007007 inner
mitochondrial membrane organization [PMID:24714493]"
/note="Protein with a role in determining mitochondrial
architecture; inner membrane protein that interacts
physically and genetically with the MICOS complex and is
required for its integrity"
/codon_start=1
/product="Aim24p"
/protein_id="NP_012614.1"
/db_xref="GeneID:853543"
/db_xref="SGD:S000003841"
/translation="MISPRVNTRVWQRSISLLSPQAAKTESNVVTKERTYIENLSKDI
ATSRFRLVDENGKIASITVQPDIPICIKKDCLVSIHNLNHLSLSYKWLNFWSNLIKFR
SFKSSLFHRIIGSSVLEILAAPNFQTSRRPFDSSRSLSVLNLTGTKDWNVFGKDSIIA
FEQNSSLEIKSPIFPSARSLVSNSSKSQLPRKFQILNGRGNVLVCGGGLVYSIELIDE
SDKILVNSRNILAINGQSQLDIANSVERQELHVEGAYVGDSSNDTVAPKFIKNQTLKS
AYGHTVQFFKRMRSWIRNQYEKRYIYGVDSYFMKIKGPRTILIQTHEMTTSKDNILTK
LTSKGHVKKSNVNDNGVNLEKQVANDVNSKIIELANRPSLFIATVSQDGRVDFQSTSK
FT"
gene complement(<581914..>582255)
/gene="EAF6"
/locus_tag="YJR082C"
/db_xref="GeneID:853544"
mRNA complement(<581914..>582255)
/gene="EAF6"
/locus_tag="YJR082C"
/product="Eaf6p"
/transcript_id="NM_001181739.3"
/db_xref="GeneID:853544"
CDS complement(581914..582255)
/gene="EAF6"
/locus_tag="YJR082C"
/experiment="EXISTENCE:curator inference:GO:0004402
histone acetyltransferase activity [PMID:17157260]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006281 DNA repair
[PMID:16135807]"
/experiment="EXISTENCE:direct assay:GO:0033100 NuA3
histone acetyltransferase complex [PMID:17157260]"
/experiment="EXISTENCE:direct assay:GO:0035267 NuA4
histone acetyltransferase complex [PMID:15485911]"
/experiment="EXISTENCE:direct assay:GO:1990467 NuA3a
histone acetyltransferase complex [PMID:25104842]"
/experiment="EXISTENCE:direct assay:GO:1990468 NuA3b
histone acetyltransferase complex [PMID:25104842]"
/note="Subunit of the NuA4 acetyltransferase complex; this
complex acetylates histone H4 and NuA3 acetyltransferase
complex that acetylates histone H3"
/codon_start=1
/product="Eaf6p"
/protein_id="NP_012615.3"
/db_xref="GeneID:853544"
/db_xref="SGD:S000003842"
/translation="MTDELKSYEALKAELKKSLQDRREQEDTFDNLQQEIYDKETEYF
SHNSNNNHSGHGGAHGSKSHYSGNIIKGFDTFSKSHHSHADSAFNNNDRIFSLSSATY
VKQQHGQSQND"
gene complement(<582608..>583537)
/gene="ACF4"
/locus_tag="YJR083C"
/db_xref="GeneID:853545"
mRNA complement(<582608..>583537)
/gene="ACF4"
/locus_tag="YJR083C"
/product="Acf4p"
/transcript_id="NM_001181740.1"
/db_xref="GeneID:853545"
CDS complement(582608..583537)
/gene="ACF4"
/locus_tag="YJR083C"
/note="hypothetical protein; computational analysis of
large-scale protein-protein interaction data suggests a
possible role in actin cytoskeleton organization;
potential Cdc28p substrate"
/codon_start=1
/product="Acf4p"
/protein_id="NP_012616.1"
/db_xref="GeneID:853545"
/db_xref="SGD:S000003843"
/translation="MSEDQRVISQPIELHKLSIVDKHSQGQQQQPHQKQHEVQPESKS
PRVTTPLKPKRLAIPISSPQRSTTNQSPVSDHASPISTDQDLIYKLAAKHREINELSF
KLEVAQKELKQLELQFKDTLPRNGQQKLGNQNPSEYLSTFTKRIQQTFVDVNNSPNML
KGKKSINDFFSKPNNNVNSNINNTLPNRKPNPPPNRSQRMQNIAPSRSSESTPTSGPP
LLPPRNTMKNANTTATAGENTPFLQRILNKFNQMNMEEDEFDDLLEKRKSKKDHYYIK
ENLGYEYDEVRSEDEDDEEFEPMGDIPVHLFKR"
gene <583733..>585004
/locus_tag="YJR084W"
/gene_synonym="CSN12"
/db_xref="GeneID:853546"
mRNA <583733..>585004
/locus_tag="YJR084W"
/gene_synonym="CSN12"
/product="uncharacterized protein"
/transcript_id="NM_001181741.1"
/db_xref="GeneID:853546"
CDS 583733..585004
/locus_tag="YJR084W"
/gene_synonym="CSN12"
/experiment="EXISTENCE:direct assay:GO:0000791 euchromatin
[PMID:21149575]"
/experiment="EXISTENCE:mutant phenotype:GO:0000398 mRNA
splicing, via spliceosome [PMID:19061648]"
/experiment="EXISTENCE:mutant phenotype:GO:0000747
conjugation with cellular fusion [PMID:12446563]"
/experiment="EXISTENCE:mutant phenotype:GO:0071444
cellular response to pheromone [PMID:12446563]"
/note="Protein that forms a complex with Thp3p; may have a
role in transcription elongation and/or mRNA splicing;
identified as a COP9 signalosome component but phenotype
and interactions suggest it may not be involved with the
signalosome"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_012617.1"
/db_xref="GeneID:853546"
/db_xref="SGD:S000003844"
/translation="MDVDIGCYFEEKRYDDKLLDFIRYDVKTPKKTKYILQRPTATDE
ESVRLQRFYQLGVDLKLKYSKRRSLKKQGRIKNATEELLRLANEQLKLFNRIVERETN
WIIYPLWVMAKQLIRLANESSELNKDSIEECGRTIHRSFTICLNDRNPRLNENKKIGC
YMFANLEFSIYHRLSNKDMIKNLVKVLESRVNARDIPPLNKSLAMEHKSQVVLYNYYL
GQYYGCLENDHERGFFHLNEALLQCPMLYVESTGKFVLQGQMEKIMILLVPLALLTKR
LYPHWDHPVIAGVITRSKRLSQVYPTLVRSVISGNLSLYEATAASHERFFLSQGLHVV
ITLLREVVFTRLVQRCWQWGNDRKSIMPLKILLATKQHDSSANEDEEEQLDALECRLA
SAIASGLLRAYLSHSNRCIVFSKKEPFPHSK"
gene complement(<585120..>585437)
/gene="TMH11"
/locus_tag="YJR085C"
/db_xref="GeneID:853547"
mRNA complement(<585120..>585437)
/gene="TMH11"
/locus_tag="YJR085C"
/product="Tmh11p"
/transcript_id="NM_001181742.3"
/db_xref="GeneID:853547"
CDS complement(585120..585437)
/gene="TMH11"
/locus_tag="YJR085C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/note="Mitochondrial hypothetical protein; GFP-fusion
protein is induced in response to the DNA-damaging agent
MMS; the authentic, non-tagged protein is detected in
highly purified mitochondria in high-throughput studies;
protein abundance increases in response to DNA replication
stress"
/codon_start=1
/product="Tmh11p"
/protein_id="NP_012618.3"
/db_xref="GeneID:853547"
/db_xref="SGD:S000003845"
/translation="MEHPAYTLSLLTTAGGLMGYYRKGSIPSLVSGLVFGSVYGIAGY
LLHMNRDGGLEMALGASTLLLGAGVIRGMPSRFTKPVPVVLTALGGLGSYYYYNKYKE
FYP"
gene <586068..>586400
/gene="STE18"
/locus_tag="YJR086W"
/db_xref="GeneID:853548"
mRNA <586068..>586400
/gene="STE18"
/locus_tag="YJR086W"
/product="Ste18p"
/transcript_id="NM_001181743.1"
/db_xref="GeneID:853548"
CDS 586068..586400
/gene="STE18"
/locus_tag="YJR086W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:8995254]"
/experiment="EXISTENCE:direct assay:GO:0005834
heterotrimeric G-protein complex [PMID:8995254]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:8995254]"
/experiment="EXISTENCE:direct assay:GO:0031680 G-protein
beta/gamma-subunit complex [PMID:8995254|PMID:8417317]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0120171
Cdc24p-Far1p-Gbetagamma complex [PMID:10087263]"
/experiment="EXISTENCE:genetic interaction:GO:0000750
pheromone-dependent signal transduction involved in
conjugation with cellular fusion [PMID:2107073]"
/experiment="EXISTENCE:genetic interaction:GO:0031680
G-protein beta/gamma-subunit complex [PMID:8417317]"
/experiment="EXISTENCE:mutant phenotype:GO:0000750
pheromone-dependent signal transduction involved in
conjugation with cellular fusion [PMID:3151178]"
/experiment="EXISTENCE:mutant phenotype:GO:0005834
heterotrimeric G-protein complex [PMID:2105453]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:8649369]"
/experiment="EXISTENCE:physical interaction:GO:0031680
G-protein beta/gamma-subunit complex [PMID:8649369]"
/experiment="EXISTENCE:physical interaction:GO:0031681
G-protein beta-subunit binding
[PMID:8649369|PMID:8417317]"
/note="G protein gamma subunit; forms dimer with Ste4p to
activate mating signaling pathway, heterotrimer with
Gpa1p, Ste4p to dampen signaling; C-terminus is
palmitoylated and farnesylated, which are required for
normal signaling; N-terminal phosphorylation of Ste18p
functions synergistically with Fus3p docking site on Ste5p
to restrict Ste5p plasma membrane recruitment and
signaling; combinatorial phosphorylation at S3 and S7, by
pheromone and pH stress, modulates Ste18p structure, Fus3p
activation"
/codon_start=1
/product="Ste18p"
/protein_id="NP_012619.1"
/db_xref="GeneID:853548"
/db_xref="SGD:S000003846"
/translation="MTSVQNSPRLQQPQEQQQQQQQLSLKIKQLKLKRINELNNKLRK
ELSRERITASNACLTIINYTSNTKDYTLPELWGYPVAGSNHFIEGLKNAQKNSQMSNS
NSVCCTLM"
gene complement(<586495..>587373)
/gene="EMC2"
/locus_tag="YJR088C"
/db_xref="GeneID:853550"
mRNA complement(<586495..>587373)
/gene="EMC2"
/locus_tag="YJR088C"
/product="Emc2p"
/transcript_id="NM_001181745.1"
/db_xref="GeneID:853550"
CDS complement(586495..587373)
/gene="EMC2"
/locus_tag="YJR088C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0072546 EMC complex
[PMID:19325107]"
/experiment="EXISTENCE:genetic interaction:GO:0034975
protein folding in endoplasmic reticulum [PMID:19325107]"
/note="Member of conserved ER transmembrane complex;
required for efficient folding of proteins in the ER; null
mutant displays induction of the unfolded protein
response; homologous to worm Y57G7A.10/EMC-2, fly CG17556,
human TTC35"
/codon_start=1
/product="Emc2p"
/protein_id="NP_012621.1"
/db_xref="GeneID:853550"
/db_xref="SGD:S000003848"
/translation="MLKDLVREKLLTIMNTKAYTQFNPEQLLQLENEMKIYMKSGDSA
LTEGNYFFLMEMLFYVLVYRNQDVDAQVVYNTLRDRLGENSYKMVIMKATLLQINGND
KGAIEYLENLLNDDLEYETDFVTYVSIAKKLIAIKTTSKNLSQESVLKEVVALTDKFP
LDAELWWYASEIYFEMGQFEKACYCLEQVLCITPFNYACFGRLSETLYYEALRSKKQT
KTELLEKALKNALRSVELSELYLKGWALVNIISRELGRNKQNDLIKLSASKLKEISAK
SNNKDKITAELILNKI"
gene <587718..>590582
/gene="BIR1"
/locus_tag="YJR089W"
/db_xref="GeneID:853551"
mRNA <587718..>590582
/gene="BIR1"
/locus_tag="YJR089W"
/product="survivin"
/transcript_id="NM_001181746.3"
/db_xref="GeneID:853551"
CDS 587718..590582
/gene="BIR1"
/locus_tag="YJR089W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:16608876]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:16608876]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005819 spindle
[PMID:16381814]"
/experiment="EXISTENCE:direct assay:GO:0005874 microtubule
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0032133 chromosome
passenger complex [PMID:19158380]"
/experiment="EXISTENCE:direct assay:GO:0051233 spindle
midzone [PMID:16381814]"
/experiment="EXISTENCE:genetic interaction:GO:0000776
kinetochore [PMID:10557299]"
/experiment="EXISTENCE:mutant phenotype:GO:0000022 mitotic
spindle elongation [PMID:16381814]"
/experiment="EXISTENCE:mutant phenotype:GO:0006915
apoptotic process [PMID:16608876]"
/experiment="EXISTENCE:mutant phenotype:GO:0007059
chromosome segregation [PMID:10557299]"
/experiment="EXISTENCE:mutant phenotype:GO:0007094 mitotic
spindle assembly checkpoint signaling [PMID:22908045]"
/experiment="EXISTENCE:mutant phenotype:GO:0031134 sister
chromatid biorientation [PMID:19528231]"
/note="Subunit of chromosomal passenger complex (CPC); CPC
is comprised of Ipl1p-Sli15p-Bir1p-Nbl1p and regulates
chromosome segregation; required for chromosome
bi-orientation and for spindle assembly checkpoint
activation upon reduced sister kinetochore tension;
relative distribution to shortened microtubules increases
upon DNA replication stress; sumoylated in an
Mms21p-dependent manner; human survivin homolog"
/codon_start=1
/product="survivin"
/protein_id="NP_012622.3"
/db_xref="GeneID:853551"
/db_xref="SGD:S000003849"
/translation="MDGQIDKMEKRYSMTKLENRLRTFQDGVALEKKKLKWSFKVIPY
QAMAKLGFYFDPVIDPKTSKLKKDSVRCCYCHRQTYNVRDCRSKRKDVLETLSNIMRQ
HLTVTDNKQVCLLIYLRNKLLTDYSFHMGVSDWKNDKYFSNPDDENVINLRKFTFQDN
WPHSGSQNEHPLGIEKMVNAGLMRYDSSIEGLGDPSMDKTLMNDTCYCIYCKQLLQGW
SINDDPMSRHYKVSQNGNCYFFQTRNRFERIKNDNDSITKNCEVSPTLGENGKREVIN
TKTASQRQCPLFESPPSSTGPQLDDYNEKTDISVIQHNISVLDGAQGENVKRNSVEEK
EQINMENGSTTLEEGNINRDVLADKKEVISTPTAKEIKRPNVQLTQSSSPIKKKRKFK
RISPRKIFDEEDSEHSLNNNSANGDNKDKDLVIDFTSHIIKNRDVGRKNAILDDSTDE
FSFSNQGHNTFDIPIPTSSHLLKGIDSDNDNVIREDDTGINTDTKGASSKHEKFSVNS
EEDLNFSEVKLTGRDSSTNILIRTQIVDQNLGDIDRDKVPNGGSPEVPKTHELIRDNS
EKREAQNGEFRHQKDSTVRQSPDILHSNKSGDNSSNITAIPKEEQRRGNSKTSSIPAD
IHPKPRKNLQEPRSLSISGKVVPTERKLDNINIDLNFSASDFSPSSQSEQSSKSSSVI
STPVASPKINLTRSLHAVKELSGLKKETDDGKYFTNKQETIKILEDVSVKNETPNNEM
LLFETGTPIASQENKSRKLFDEEFSGKELDIPIDSSTVEIKKVIKPEFEPVPSVARNL
VSGTSSYPRNSRLEEQRKETSTSLADNSKKGSSFNEGNNEKEPNAAEWFKIDENRHLV
KNYFHDLLKYINNNDATLANDKDGDLAFLIKQMPAEELDMTFNNWVNLKVQSIKREFI
DDCDKKLDILRRDYYTATNFIETLEDDNQLIDIAKKMGIL"
gene complement(<590872..>594327)
/gene="GRR1"
/locus_tag="YJR090C"
/gene_synonym="CAT80; COT2; RMR3; SDC1; SSU2"
/db_xref="GeneID:853552"
mRNA complement(<590872..>594327)
/gene="GRR1"
/locus_tag="YJR090C"
/gene_synonym="CAT80; COT2; RMR3; SDC1; SSU2"
/product="SCF ubiquitin ligase complex subunit GRR1"
/transcript_id="NM_001181747.1"
/db_xref="GeneID:853552"
CDS complement(590872..594327)
/gene="GRR1"
/locus_tag="YJR090C"
/gene_synonym="CAT80; COT2; RMR3; SDC1; SSU2"
/experiment="EXISTENCE:direct assay:GO:0000142 cellular
bud neck contractile ring [PMID:11080155|PMID:15775961]"
/experiment="EXISTENCE:direct assay:GO:0004842
ubiquitin-protein transferase activity [PMID:10213692]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11080155]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11080155]"
/experiment="EXISTENCE:direct assay:GO:0030674
protein-macromolecule adaptor activity
[PMID:9346238|PMID:9819356|PMID:11259599]"
/experiment="EXISTENCE:genetic interaction:GO:0000209
protein polyubiquitination [PMID:9499404]"
/experiment="EXISTENCE:mutant phenotype:GO:0000209 protein
polyubiquitination [PMID:16093347]"
/experiment="EXISTENCE:mutant phenotype:GO:0000751 mitotic
cell cycle G1 arrest in response to pheromone
[PMID:15942932]"
/experiment="EXISTENCE:mutant phenotype:GO:0019005 SCF
ubiquitin ligase complex [PMID:9736614]"
/experiment="EXISTENCE:mutant phenotype:GO:0030674
protein-macromolecule adaptor activity [PMID:9819356]"
/experiment="EXISTENCE:mutant phenotype:GO:0031146
SCF-dependent proteasomal ubiquitin-dependent protein
catabolic process [PMID:29212878]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress [PMID:29212878]"
/experiment="EXISTENCE:mutant phenotype:GO:0071406
cellular response to methylmercury [PMID:17141224]"
/experiment="EXISTENCE:physical interaction:GO:0031146
SCF-dependent proteasomal ubiquitin-dependent protein
catabolic process [PMID:10213692]"
/note="F-box protein component of an SCF ubiquitin-ligase
complex; modular substrate specificity factor which
associates with core SCF (Cdc53p, Skp1p and Hrt1p/Rbx1p)
to form the SCF(Grr1) complex; SCF(Grr1) acts as a
ubiquitin-protein ligase directing ubiquitination of
substrates such as: Gic2p, Mks1p, Mth1p, Cln1p, Cln2p and
Cln3p; involved in carbon catabolite repression,
glucose-dependent divalent cation transport, glucose
transport, morphogenesis, and sulfite detoxification"
/codon_start=1
/product="SCF ubiquitin ligase complex subunit GRR1"
/protein_id="NP_012623.1"
/db_xref="GeneID:853552"
/db_xref="SGD:S000003850"
/translation="MDQDNNNHNDSNRLHPPDIHPNLGPQLWLNSSGDFDDNNNNNNN
NNNNNSTRPQMPSRTRETATSERNASEVRDATLNNIFRFDSIQRETLLPTNNGQPLNQ
NFSLTFQPQQQTNALNGIDINTVNTNLMNGVNVQIDQLNRLLPNLPEEERKQIHEFKL
IVGKKIQEFLVVIEKRRKKILNEIELDNLKLKELRIDNSPQAISYLHKLQRMRLRALE
TENMEIRNLRLKILTIIEEYKKSLYAYCHSKLRGQQVENPTDNFIIWINSIDTTESSD
LKEGLQDLSRYSRQFINNVLSNPSNQNICTSVTRRSPVFALNMLPSEILHLILDKLNQ
KYDIVKFLTVSKLWAEIIVKILYYRPHINKKSQLDLFLRTMKLTSEETVFNYRLMIKR
LNFSFVGDYMHDTELNYFVGCKNLERLTLVFCKHITSVPISAVLRGCKFLQSVDITGI
RDVSDDVFDTLATYCPRVQGFYVPQARNVTFDSLRNFIVHSPMLKRIKITANNNMNDE
LVELLANKCPLLVEVDITLSPNVTDSSLLKLLTRLVQLREFRITHNTNITDNLFQELS
KVVDDMPSLRLIDLSGCENITDKTIESIVNLAPKLRNVFLGKCSRITDASLFQLSKLG
KNLQTVHFGHCFNITDNGVRALFHSCTRIQYVDFACCTNLTNRTLYELADLPKLKRIG
LVKCTQMTDEGLLNMVSLRGRNDTLERVHLSYCSNLTIYPIYELLMSCPRLSHLSLTA
VPSFLRPDITMYCRPAPSDFSENQRQIFCVFSGKGVHKLRHYLVNLTSPAFGPHVDVN
DVLTKYIRSKNLIFNGETLEDALRRIITDLNQDSAAIIAATGLNQINGLNNDFLFQNI
NFERIDEVFSWYLNTFDGIRMSSEEVNSLLLQVNKTFCEDPFSDVDDDQDYVVAPGVN
REINSEMCHIVRKFHELNDHIDDFEVNVASLVRVQFQFTGFLLHEMTQTYMQMIELNR
QICLVQKTVQESGNIDYQKGLLIWRLLFIDKFIMVVQKYKLSTVVLRLYLKDNITLLT
RQRELLIAHQRSAWNNNNDNDANRNANNIVNIVSDAGANDTSNNETNNGNDDNETENP
NFWRQFGNRMQISPDQMRNLQMGLRNQNMVRNNNNNTIDESMPDTAIDSQMDEASGTP
DEDML"
gene complement(<595061..>598336)
/gene="JSN1"
/locus_tag="YJR091C"
/gene_synonym="PUF1"
/db_xref="GeneID:853553"
mRNA complement(<595061..>598336)
/gene="JSN1"
/locus_tag="YJR091C"
/gene_synonym="PUF1"
/product="Jsn1p"
/transcript_id="NM_001181748.1"
/db_xref="GeneID:853553"
CDS complement(595061..598336)
/gene="JSN1"
/locus_tag="YJR091C"
/gene_synonym="PUF1"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:15024427|PMID:21685478|PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0032473 cytoplasmic
side of mitochondrial outer membrane [PMID:16107558]"
/experiment="EXISTENCE:genetic interaction:GO:0000288
nuclear-transcribed mRNA catabolic process,
deadenylation-dependent decay [PMID:11101532]"
/experiment="EXISTENCE:mutant phenotype:GO:0031503
protein-containing complex localization [PMID:16107558]"
/experiment="EXISTENCE:mutant phenotype:GO:0051654
establishment of mitochondrion localization
[PMID:16107558]"
/note="Member of the Puf family of RNA-binding proteins;
interacts with mRNAs encoding membrane-associated
proteins; involved in localizing the Arp2/3 complex to
mitochondria; overexpression causes increased sensitivity
to benomyl; JSN1 has a paralog, PUF2, that arose from the
whole genome duplication"
/codon_start=1
/product="Jsn1p"
/protein_id="NP_012624.1"
/db_xref="GeneID:853553"
/db_xref="SGD:S000003851"
/translation="MDKSKQMNINNLSNIPEVIDPGITIPIYEEEYENNGESNSQLQQ
QPQKLGSYRSRAGKFSNTLSNLLPSISAKLHHSKKNSHGKNGAEFSSSNNSSQSTVAS
KTPRASPSRSKMMESSIDGVTMDRPGSLTPPQDMEKLVHFPDSSNNFLIPAPRGSSDS
FNLPHQISRTRNNTMSSQITSISSIAPKPRTSSGIWSSNASANDPMQQHLLQQLQPTT
SNNTTNSNTLNDYSTKTAYFDNMVSTSGSQMADNKMNTNNLAIPNSVWSNTRQRSQSN
ASSIYTDAPLYEQPARASISSHYTIPTQESPLIADEIDPQSINWVTMDPTVPSINQIS
NLLPTNTISISNVFPLQHQQPQLNNAINLTSTSLATLCSKYGEVISARTLRNLNMALV
EFSSVESAVKALDSLQGKEVSMIGAPSKISFAKILPMHQQPPQFLLNSQGLPLGLENN
NLQPQPLLQEQLFNGAVTFQQQGNVSIPVFNQQSQQSQHQNHSSGSAGFSNVLHGYNN
NNSMHGNNNNSANEKEQCPFPLPPPNVNEKEDLLREIIELFEANSDEYQINSLIKKSL
NHKGTSDTQNFGPLPEPLSGREFDPPKLRELRKSIDSNAFSDLEIEQLAIAMLDELPE
LSSDYLGNTIVQKLFEHSSDIIKDIMLRKTSKYLTSMGVHKNGTWACQKMITMAHTPR
QIMQVTQGVKDYCTPLINDQFGNYVIQCVLKFGFPWNQFIFESIIANFWVIVQNRYGA
RAVRACLEAHDIVTPEQSIVLSAMIVTYAEYLSTNSNGALLVTWFLDTSVLPNRHSIL
APRLTKRIVELCGHRLASLTILKVLNYRGDDNARKIILDSLFGNVNAHDSSPPKELTK
LLCETNYGPTFVHKVLAMPLLEDDLRAHIIKQVRKVLTDSTQIQPSRRLLEEVGLASP
SSTHNKTKQQQQQHHNSSISHMFATPDTSGQHMRGLSVSSVKSGGSKHTTMNTTTTNG
SSASTLSPGQPLNANSNSSMGYFSYPGVFPVSGFSGNASNGYAMNNDDLSSQFDMLNF
NNGTRLSLPQLSLTNHNNTTMELVNNVGSSQPHTNNNNNNNNTNYNDDNTVFETLTLH
SAN"
gene <598735..>603078
/gene="BUD4"
/locus_tag="YJR092W"
/db_xref="GeneID:853554"
mRNA <598735..>603078
/gene="BUD4"
/locus_tag="YJR092W"
/product="Bud4p"
/transcript_id="NM_001181749.3"
/db_xref="GeneID:853554"
CDS 598735..603078
/gene="BUD4"
/locus_tag="YJR092W"
/experiment="EXISTENCE:direct assay:GO:0000142 cellular
bud neck contractile ring [PMID:22553209]"
/experiment="EXISTENCE:direct assay:GO:0005525 GTP binding
[PMID:22553209]"
/experiment="EXISTENCE:direct assay:GO:0005621 cellular
bud scar [PMID:23345395]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:27385335|PMID:22842922]"
/experiment="EXISTENCE:mutant phenotype:GO:0005525 GTP
binding [PMID:22553209]"
/experiment="EXISTENCE:mutant phenotype:GO:0007120 axial
cellular bud site selection [PMID:22553209]"
/experiment="EXISTENCE:mutant phenotype:GO:0031106 septin
ring organization [PMID:23345395]"
/experiment="EXISTENCE:mutant phenotype:GO:0097271 protein
localization to bud neck [PMID:22553209]"
/note="Anillin-like protein involved in bud-site
selection; required for the axial budding pattern;
required for the formation and disassembly of the double
septin ring structure, and generally for septin
organization; functions as a platform linking the
cytokinesis tag septins to the axial landmark through its
multiple domains; in vivo substrate of Cdc28p/Clb2p"
/codon_start=1
/product="Bud4p"
/protein_id="NP_012625.5"
/db_xref="GeneID:853554"
/db_xref="SGD:S000003852"
/translation="MHDAESTVDSLLKEIDNEMEQTKSNITQNGSEDTPHNWKLPLQE
IGDDTMEMLVKHNTRSNATENSRGRSPSKMSTISNESLNLGLLRVNSELEESPAAVHQ
ERIKNSVANGALGHANSPKVLNNLKNMAQDIDKLARDEEKPVKLSSSPLKFTLKSTQP
LLSYPESPIHRSSIEIETNYDDEDEEEEDAYTCLTQSPQILHSPSRIPITNAVSINKL
NLDFTLNPNESDKSLVSDTSVDSTGRELDTKTIPELPFCMSSTPEMTPVDEKCNLPSK
LLNTSNNSHSDSRSPTASVEDLNISTNLPGADSSQNNPVTTDADALIENDVVRDLQQN
MEHIDDAFDEKKVLDEGCSNEPVTFLGENDTRSIVYSNKGTNANVQEFSQEDSLAHSE
PKFKDLNATSDDVWNEDKETDANISTSTKSEESYIADYKVTRQEDWDTKKLHQESEHA
NEQPAIIPQKDSSEETFTELNNESEFQRNFKDGEEYRIVQHEESLYGQRTKSPEENII
NGSEIGVDHGEAAEVNEPLAKTSAEEHDLSSSCEDQSVSEARNKDRIEEKEVETKDEN
IETEKDESEYHKVEENEEPEHVPLLPPLPRWEEIQFNEPFIDENDTSNDSIDLTRSMK
PSDYISIWHIQEEEIKSNSPESIANSQFSQQSSITTASTVDSKKDNGSTSFKFKPRIV
SRSRIYNPKSRVSSLNYYDNEDYILSNSEWNALDPMRRNTLISKRIQDNIRTQKGHAP
LIRPSIMKLNGEDSGFQNHFLEVEQPQEHENIPLSTHLSEQDITTNVGLDEQKLPTNT
QDEAEISIREIESAGDITFNRGDLLSLSFDEELGQDFANFLDALDHDSTSFNHGPDDS
SSFQRDSSKKSFNSLWESSYELKPPPSIRKQPIAPDVLQKLLESDTKDDADLEKIREE
RITEPRTGLGIGMLKTPVKDVSIALAASIKGYEASFSDTDSRPEGMNNSDAITLNMFD
DFEEDKMTPSTPVRSISPIKRHVSSPFKVVKAGNKQENNEINIKAEEEIEPMTQQETD
GLKQDIPPLLAQTKDNVEAKEETITQLEEPQDVEQEFPDMGTLYLSIKAISTLALYGT
KSHRATYAIVFDNGENVVQTPWESLPYDGNIRINKEFELPIDFKGKAETSSASSERDS
YKKCVITLKCKYEKPRHELVEIVDKVPVGKSFFGKTKYKFEKKYVQKKPKQDEWDYLF
AQDGSFARCEIEINEEFLKNVAFNTSHMHYNMINKWSRIADKIHGSKRLYELPRKAPH
KVASLDVEACFLERTSAFEQFPKQFSLVNKIVSKYKLQQNIYKEGYLLQDGGDLKGKI
ENRFFKLHGSQLSGYHEISRKAKIDINLLKVTKVLRNEDIQADNGGQRNFTDWVLFNE
CFQLVFDDGERITFNAECSNEEKSDWYNKLQEVVELNVFHQPWVKKYCEKLAEEEKTR
TTGHNLKQDFN"
gene complement(<603220..>604203)
/gene="FIP1"
/locus_tag="YJR093C"
/db_xref="GeneID:853555"
mRNA complement(<603220..>604203)
/gene="FIP1"
/locus_tag="YJR093C"
/product="cleavage polyadenylation factor subunit FIP1"
/transcript_id="NM_001181750.1"
/db_xref="GeneID:853555"
CDS complement(603220..604203)
/gene="FIP1"
/locus_tag="YJR093C"
/experiment="EXISTENCE:direct assay:GO:0005847 mRNA
cleavage and polyadenylation specificity factor complex
[PMID:12819204]"
/experiment="EXISTENCE:direct assay:GO:0006397 mRNA
processing [PMID:11344258]"
/experiment="EXISTENCE:genetic interaction:GO:0030674
protein-macromolecule adaptor activity [PMID:21282348]"
/experiment="EXISTENCE:mutant phenotype:GO:0003723 RNA
binding [PMID:11238938]"
/experiment="EXISTENCE:mutant phenotype:GO:0030674
protein-macromolecule adaptor activity [PMID:21282348]"
/experiment="EXISTENCE:physical interaction:GO:0030674
protein-macromolecule adaptor activity [PMID:7736590]"
/note="Subunit of cleavage polyadenylation factor (CPF);
interacts directly with poly(A) polymerase (Pap1p) to
regulate its activity; bridging factor that links Pap1p
and the CPF complex via Yth1p"
/codon_start=1
/product="cleavage polyadenylation factor subunit FIP1"
/protein_id="NP_012626.1"
/db_xref="GeneID:853555"
/db_xref="SGD:S000003853"
/translation="MSSSEDEDDKFLYGSDSELALPSSKRSRDDEADAGASSNPDIVK
RQKFDSPVEETPATARDDRSDEDIYSDSSDDDSDSDLEVIISLGPDPTRLDAKLLDSY
STAATSSSKDVISVATDVSNTITKTSDERLITEGEANQGVTATTVKATESDGNVPKAM
TGSIDLDKEGIFDSVGITTIDPEVLKEKPWRQPGANLSDYFNYGFNEFTWMEYLHRQE
KLQQDYNPRRILMGLLSLQQQGKLNSANDTDSNLGNIIDNNNNVNNANMSNLNSNMGN
SMSGTPNPPAPPMHPSFPPLPMFGSFPPFPMPGMMPPMNQQPNQNQNQNSK"
gene complement(<604569..>605651)
/gene="IME1"
/locus_tag="YJR094C"
/db_xref="GeneID:853556"
mRNA complement(<604569..>605651)
/gene="IME1"
/locus_tag="YJR094C"
/product="transcription factor IME1"
/transcript_id="NM_001181751.1"
/db_xref="GeneID:853556"
CDS complement(604569..605651)
/gene="IME1"
/locus_tag="YJR094C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:8462841]"
/experiment="EXISTENCE:direct assay:GO:0045944 positive
regulation of transcription by RNA polymerase II
[PMID:29472539]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:8205723]"
/experiment="EXISTENCE:mutant phenotype:GO:0007124
pseudohyphal growth [PMID:20876298]"
/experiment="EXISTENCE:mutant phenotype:GO:0040020
regulation of meiotic nuclear division [PMID:8205723]"
/experiment="EXISTENCE:mutant phenotype:GO:0043934
sporulation [PMID:9660952]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:9660952]"
/experiment="EXISTENCE:physical interaction:GO:0008134
transcription factor binding [PMID:8628320]"
/note="Master meiotic regulator active only during meiotic
events; activates transcription of early meiotic genes
through interaction with Ume6p; regulator of meiotic
commitment; phosphorylated by Rim11p; degraded by the 26S
proteasome following phosphorylation by Ime2p;
transcription is negatively regulated in cis by the IRT1
long noncoding antisense RNA"
/codon_start=1
/product="transcription factor IME1"
/protein_id="NP_012627.1"
/db_xref="GeneID:853556"
/db_xref="SGD:S000003854"
/translation="MQADMHGKLHAALEDGFFLFPFEQQQQPNIYYDTTTDQEDRPCF
SFGSTISPRSWHFEKSDKIASSQLQNLVHTQPIHLINPQILFNEEFLNLENIDSQPIS
KETKTTKDCTMATGPERGKKSSESTRSSSLSSLFSNDESASTFHSSFNNHDNFQKSNR
NGDDIDISDTIKYETNTNAQKDIKIFQENFEFNEFPYTQDFYPYTTNYTYSKPTNIHE
SINSKNTDSYSQYQDQFPPHTDNIHSFNNRHYSNHKSTNCNYYNNTSNNNNASDNVYE
ADPFIDEPQVPSYYYPLEIAFDVEKSPPPSLQKLNSKELEFLKKLNSKLSRYAAAYSF
SSSNDQDYYDKVRFQEISYKFSKTYS"
gene complement(605936..607424)
/gene="IRT1"
/locus_tag="YNCJ0028C"
/db_xref="GeneID:23547381"
ncRNA complement(605936..607424)
/ncRNA_class="other"
/gene="IRT1"
/locus_tag="YNCJ0028C"
/product="IRT1"
/note="Long noncoding RNA that governs mating-type control
of gametogenesis; located in the IME1 promoter; in
haploids, expression of IME1, the central inducer of
gametogenesis, is inhibited in cis by transcription of
IRT1, which recruits the Set2p histone methyltransferase
and the Set3p histone deacetylase complex to establish
repressive chromatin at the IME1 promoter"
/transcript_id="NR_132197.1"
/db_xref="GeneID:23547381"
/db_xref="SGD:S000178119"
gene <608305..>608858
/gene="RPL43B"
/locus_tag="YJR094W-A"
/db_xref="GeneID:853557"
mRNA join(<608305..608306,608582..>608858)
/gene="RPL43B"
/locus_tag="YJR094W-A"
/product="60S ribosomal protein eL43 RPL43B"
/transcript_id="NM_001181752.3"
/db_xref="GeneID:853557"
CDS join(608305..608306,608582..608858)
/gene="RPL43B"
/locus_tag="YJR094W-A"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:11983894]"
/experiment="EXISTENCE:curator inference:GO:0003735
structural constituent of ribosome [PMID:11983894]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:11983894]"
/note="Ribosomal 60S subunit protein L43B; homologous to
mammalian ribosomal protein L37A, no bacterial homolog;
RPL43B has a paralog, RPL43A, that arose from the whole
genome duplication; protein abundance increases in
response to DNA replication stress"
/codon_start=1
/product="60S ribosomal protein eL43 RPL43B"
/protein_id="NP_012628.3"
/db_xref="GeneID:853557"
/db_xref="SGD:S000003855"
/translation="MAKRTKKVGITGKYGVRYGSSLRRQVKKLEIQQHARYDCSFCGK
KTVKRGAAGIWTCSCCKKTVAGGAYTVSTAAAATVRSTIRRLREMVEA"
gene <609773..>610741
/gene="SFC1"
/locus_tag="YJR095W"
/gene_synonym="ACR1"
/db_xref="GeneID:853558"
mRNA <609773..>610741
/gene="SFC1"
/locus_tag="YJR095W"
/gene_synonym="ACR1"
/product="Sfc1p"
/transcript_id="NM_001181753.1"
/db_xref="GeneID:853558"
CDS 609773..610741
/gene="SFC1"
/locus_tag="YJR095W"
/gene_synonym="ACR1"
/experiment="EXISTENCE:direct assay:GO:0005469
succinate:fumarate antiporter activity [PMID:9395087]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0015741 fumarate
transport [PMID:9395087]"
/experiment="EXISTENCE:direct assay:GO:0015744 succinate
transport [PMID:9395087]"
/note="Mitochondrial succinate-fumarate transporter;
transports succinate into and fumarate out of the
mitochondrion; required for ethanol and acetate
utilization"
/codon_start=1
/product="Sfc1p"
/protein_id="NP_012629.1"
/db_xref="GeneID:853558"
/db_xref="SGD:S000003856"
/translation="MSQKKKASHPAINLMAGGTAGLFEALCCHPLDTIKVRMQIYRRV
AGIEHVKPPGFIKTGRTIYQKEGFLALYKGLGAVVIGIIPKMAIRFSSYEFYRTLLVN
KESGIVSTGNTFVAGVGAGITEAVLVVNPMEVVKIRLQAQHLTPSEPNAGPKYNNAIH
AAYTIVKEEGVSALYRGVSLTAARQATNQGANFTVYSKLKEFLQNYHQMDVLPSWETS
CIGLISGAIGPFSNAPLDTIKTRLQKDKSISLEKQSGMKKIITIGAQLLKEEGFRALY
KGITPRVMRVAPGQAVTFTVYEYVREHLENLGIFKKNDTPKPKPLK"
gene <611195..>612043
/locus_tag="YJR096W"
/db_xref="GeneID:853559"
mRNA <611195..>612043
/locus_tag="YJR096W"
/product="aldo-keto reductase superfamily protein"
/transcript_id="NM_001181754.1"
/db_xref="GeneID:853559"
CDS 611195..612043
/locus_tag="YJR096W"
/experiment="EXISTENCE:direct assay:GO:0004032 aldose
reductase (NADPH) activity [PMID:11306085|PMID:12271459]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0008106 alcohol
dehydrogenase (NADP+) activity [PMID:17140678]"
/experiment="EXISTENCE:direct assay:GO:0019568 arabinose
catabolic process [PMID:12271459]"
/experiment="EXISTENCE:genetic interaction:GO:0034599
cellular response to oxidative stress [PMID:17919749]"
/experiment="EXISTENCE:mutant phenotype:GO:0019568
arabinose catabolic process [PMID:12271459]"
/experiment="EXISTENCE:mutant phenotype:GO:0042843
D-xylose catabolic process [PMID:12271459]"
/note="Xylose and arabinose reductase; member of the
aldo-keto reductase (AKR) family; GFP-fusion protein is
induced in response to the DNA-damaging agent MMS"
/codon_start=1
/product="aldo-keto reductase superfamily protein"
/protein_id="NP_012630.1"
/db_xref="GeneID:853559"
/db_xref="SGD:S000003857"
/translation="MVPKFYKLSNGFKIPSIALGTYDIPRSQTAEIVYEGVKCGYRHF
DTAVLYGNEKEVGDGIIKWLNEDPGNHKREEIFYTTKLWNSQNGYKRAKAAIRQCLNE
VSGLQYIDLLLIHSPLEGSKLRLETWRAMQEAVDEGLVKSIGVSNYGKKHIDELLNWP
ELKHKPVVNQIEISPWIMRQELADYCKSKGLVVEAFAPLCHGYKMTNPDLLKVCKEVD
RNPGQVLIRWSLQHGYLPLPKTKTVKRLEGNLAAYNFELSDEQMKFLDHPDAYEPTDW
ECTDAP"
gene <612413..>612931
/gene="JJJ3"
/locus_tag="YJR097W"
/gene_synonym="DPH4"
/db_xref="GeneID:853560"
mRNA <612413..>612931
/gene="JJJ3"
/locus_tag="YJR097W"
/gene_synonym="DPH4"
/product="Jjj3p"
/transcript_id="NM_001181755.3"
/db_xref="GeneID:853560"
CDS 612413..612931
/gene="JJJ3"
/locus_tag="YJR097W"
/gene_synonym="DPH4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008198 ferrous
iron binding [PMID:22367199]"
/experiment="EXISTENCE:mutant phenotype:GO:0017183 protein
histidyl modification to diphthamide [PMID:15485916]"
/note="Iron-binding protein; involved in targeting
cytoplasmic SRP-independent proteins to the ER; contains a
CSL Zn finger and a DnaJ-domain; involved in diphthamide
biosynthesis; ortholog human Dph4"
/codon_start=1
/product="Jjj3p"
/protein_id="NP_012631.3"
/db_xref="GeneID:853560"
/db_xref="SGD:S000003858"
/translation="MSLVNSLTHYEILRIPSDATQDEIKKAYRNRLLNTHPDKLSKSI
HDTVSNVTINKIQDAYKILSNIKTRREYDRLILENYKRQGFHNCGDGLDEFSLDDFSF
DEDKLEFMMNCPRCQFVGGFHFSESLLDECIDNVDAMERSHSGYQLLTQCSACSLWLK
VNFDIEEEQEGQ"
rep_origin 612846..613278
/note="ARS1019; Autonomously Replicating Sequence"
/db_xref="SGD:S000028456"
gene complement(<613186..>615156)
/locus_tag="YJR098C"
/db_xref="GeneID:853561"
mRNA complement(<613186..>615156)
/locus_tag="YJR098C"
/product="uncharacterized protein"
/transcript_id="NM_001181756.2"
/db_xref="GeneID:853561"
CDS complement(613186..615156)
/locus_tag="YJR098C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/note="hypothetical protein; the authentic, non-tagged
protein is detected in highly purified mitochondria in
high-throughput studies"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_012632.2"
/db_xref="GeneID:853561"
/db_xref="SGD:S000003859"
/translation="MMATPATDLISDNDKYNKQCLSDSSDSGSDVSFFSVNESEGELD
TMEKVDTLIGGARVISNKVEKDSDSEQRGRKKETTGPNNYHNLEEKQASAISLDADDE
DLDEIISYSHDGNYDSSHKTFSFSLPFGNTNFRSSSPLAIIKTVLPKTPDEFIKKNLR
KNEIKQKLKKSTSISSLEEIELFKYERGIDNSRLRAVKESLEMDALKNSIKQITADPF
DKTHDGYYRSRLESIWNELEGDVVIMGGYRGSVLRDATTHKRIWIPLKAGLNMTKVDL
LIGPNDEDELKTQKEIVPDGMLTHIGPVDISKRLIKRLDANPNLNVQQFGYDWRLSLD
ISAKHLTTKLEEIYNKQKNKKGIYIIAHSMGGLVAHKVLQDCTHLIRGIIYVGSPSQC
PNILGPIRFGDDVMWNKTIFTKETNFFMRSSFYFLPLDGRCFVDKITLERYDFDFFDT
DVWKTLGLSPLVNEKREESAHEKSKLLPRKTKSALSLKATLNATTKFVLNAPVVRNVA
GNNKQVPRDVPFDEVFHTSYEDSCEYLARTLKRTKNYLDSLDYDPNKEYPPLAMVYGN
KVPTVRGAKVNGIQDIKDGNYEDFYYGPGDGVVHHKWLLPEQRGFPVVCKIASSSGHV
SLMTDLKSMAKAFISIVDSEKEGRRSRTRTSS"
gene <615576..>616286
/gene="YUH1"
/locus_tag="YJR099W"
/db_xref="GeneID:853562"
mRNA <615576..>616286
/gene="YUH1"
/locus_tag="YJR099W"
/product="ubiquitin-specific protease YUH1"
/transcript_id="NM_001181757.1"
/db_xref="GeneID:853562"
CDS 615576..616286
/gene="YUH1"
/locus_tag="YJR099W"
/EC_number="3.4.19.12"
/experiment="EXISTENCE:curator inference:GO:0005737
cytoplasm [PMID:1429680]"
/experiment="EXISTENCE:curator inference:GO:0010992
ubiquitin recycling [PMID:1429680]"
/experiment="EXISTENCE:direct assay:GO:0004843
cysteine-type deubiquitinase activity [PMID:1429680]"
/experiment="EXISTENCE:direct assay:GO:0051604 protein
maturation [PMID:40347692]"
/note="Ubiquitin C-terminal hydrolase; cleaves
ubiquitin-protein fusions to generate monomeric ubiquitin;
hydrolyzes the peptide bond at the C-terminus of
ubiquitin; also the major processing enzyme for the
ubiquitin-like protein Rub1p"
/codon_start=1
/product="ubiquitin-specific protease YUH1"
/protein_id="NP_012633.1"
/db_xref="GeneID:853562"
/db_xref="SGD:S000003860"
/translation="MSGENRAVVPIESNPEVFTNFAHKLGLKNEWAYFDIYSLTEPEL
LAFLPRPVKAIVLLFPINEDRKSSTSQQITSSYDVIWFKQSVKNACGLYAILHSLSNN
QSLLEPGSDLDNFLKSQSDTSSSKNRFDDVTTDQFVLNVIKENVQTFSTGQSEAPEAT
ADTNLHYITYVEENGGIFELDGRNLSGPLYLGKSDPTATDLIEQELVRVRVASYMENA
NEEDVLNFAMLGLGPNWE"
gene complement(<616351..>617334)
/gene="AIM25"
/locus_tag="YJR100C"
/gene_synonym="SLM35"
/db_xref="GeneID:853563"
mRNA complement(<616351..>617334)
/gene="AIM25"
/locus_tag="YJR100C"
/gene_synonym="SLM35"
/product="Aim25p"
/transcript_id="NM_001181758.3"
/db_xref="GeneID:853563"
CDS complement(616351..617334)
/gene="AIM25"
/locus_tag="YJR100C"
/gene_synonym="SLM35"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:genetic interaction:GO:0034599
cellular response to oxidative stress [PMID:27922823]"
/experiment="EXISTENCE:genetic interaction:GO:0034605
cellular response to heat [PMID:27922823]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress [PMID:27922823]"
/experiment="EXISTENCE:mutant phenotype:GO:1903147
negative regulation of autophagy of mitochondrion
[PMID:27922823]"
/note="Mitochondria hypothetical protein; interacts
genetically with TOR1 to regulate chronological lifespan,
and the response to both heat shock and oxidative stress;
involved in maintaining the integrity of the mitochondrial
network; negative regulator of mitophagy flux; non-tagged
protein is detected in purified mitochondria in
high-throughput studies; null mutant is viable and
displays an elevated frequency of mitochondrial genome
loss; similar to murine NOR1"
/codon_start=1
/product="Aim25p"
/protein_id="NP_012634.3"
/db_xref="GeneID:853563"
/db_xref="SGD:S000003861"
/translation="MHRTAIFLTYRSCMRNFSTLSKTLTVSSGKVIRNGPFRRVIREK
NQITKAPSVKAFKENSNSGIIKVHDPIATTILNEPTVIIERQIEFMNVFLGFEQANRY
AIMDVNGNKIASMMERDFSITKAIMRQFYRLHRPFLVDVFDNWGNVIMTIKRPFSFIN
SHIKTIIPPSAYVDNGSDSTHYHDGKEGTTVGETIQNWHLWRRRYELFQKDGVEGSTF
DQFGKIDAPFLSFDFPVTDADGKIMASVDRNWVGLGREMFTDTGVYVVRFDSQRCFDN
IYPTEMLSSQVLTLDQRAVLLANAVSIDFDYFSRHSRQTGGFLSFGGGYDE"
gene 617919..618019
/locus_tag="YNCJ0029W"
/db_xref="GeneID:853564"
tRNA join(617919..617956,617976..618019)
/locus_tag="YNCJ0029W"
/product="tRNA-Leu"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:853564"
/db_xref="SGD:S000006654"
gene <618234..>619034
/gene="RSM26"
/locus_tag="YJR101W"
/gene_synonym="mS42"
/db_xref="GeneID:853565"
mRNA <618234..>619034
/gene="RSM26"
/locus_tag="YJR101W"
/gene_synonym="mS42"
/product="mitochondrial 37S ribosomal protein mS42 RSM26"
/transcript_id="NM_001181759.1"
/db_xref="GeneID:853565"
CDS 618234..619034
/gene="RSM26"
/locus_tag="YJR101W"
/gene_synonym="mS42"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:11278769]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:11278769]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005763
mitochondrial small ribosomal subunit [PMID:11278769]"
/note="Mitochondrial ribosomal protein of the small
subunit"
/codon_start=1
/product="mitochondrial 37S ribosomal protein mS42 RSM26"
/protein_id="NP_012635.1"
/db_xref="GeneID:853565"
/db_xref="SGD:S000003862"
/translation="MLVFKRGIHVVPKLPNSKALLQNGVPNILSSSGFKTVWFDYQRY
LCDKLTLATAGQSLESYYPFHILLKTAGNPLQSNIFNLASSIHNNHLFVENILPSAVE
HGTNSNAVVKTEPSRLFLSKIKDSFNGSDWEVVKEEMIYRAENEVLGQGWLFLVENNE
KKLFILTSNNNGTPYYFPRNQSFDLNSAISIDEFATLKQMKELIGKSTKLNGKVQDWT
MPIICVNLWDHAYLHDYGVGNRSKYVKNVLDNLNWSVVNNRIFSGISK"
gene complement(<619157..>619765)
/gene="VPS25"
/locus_tag="YJR102C"
/gene_synonym="VPL12; VPT25"
/db_xref="GeneID:853566"
mRNA complement(<619157..>619765)
/gene="VPS25"
/locus_tag="YJR102C"
/gene_synonym="VPL12; VPT25"
/product="ESCRT-II subunit protein VPS25"
/transcript_id="NM_001181760.1"
/db_xref="GeneID:853566"
CDS complement(619157..619765)
/gene="VPS25"
/locus_tag="YJR102C"
/gene_synonym="VPL12; VPT25"
/experiment="EXISTENCE:direct assay:GO:0000814 ESCRT II
complex [PMID:12194858]"
/experiment="EXISTENCE:direct assay:GO:0005198 structural
molecule activity [PMID:15469844|PMID:15329733]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:11278625]"
/experiment="EXISTENCE:mutant phenotype:GO:0005198
structural molecule activity
[PMID:15329733|PMID:15469844]"
/experiment="EXISTENCE:mutant phenotype:GO:0006623 protein
targeting to vacuole [PMID:12194858]"
/experiment="EXISTENCE:mutant phenotype:GO:1904669 ATP
export [PMID:26585826]"
/note="Component of the ESCRT-II complex; ESCRT-II is
involved in ubiquitin-dependent sorting of proteins into
the endosome"
/codon_start=1
/product="ESCRT-II subunit protein VPS25"
/protein_id="NP_012636.1"
/db_xref="GeneID:853566"
/db_xref="SGD:S000003863"
/translation="MSALPPVYSFPPLYTRQPNSLTRRQQISTWIDIISQYCKTKKIW
YMSVDGTVINDNELDSGSTDNDDSKKISKNLFNNEDIQRSVSQVFIDEIWSQMTKEGK
CLPIDQSGRRSSNTTTTRYFILWKSLDSWASLILQWFEDSGKLNQVITLYELSEGDET
VNWEFHRMPESLLYYCLKPLCDRNRATMLKDENDKVIAIKVV"
gene <620754..>622490
/gene="URA8"
/locus_tag="YJR103W"
/db_xref="GeneID:853567"
mRNA <620754..>622490
/gene="URA8"
/locus_tag="YJR103W"
/product="CTP synthase URA8"
/transcript_id="NM_001181761.4"
/db_xref="GeneID:853567"
CDS 620754..622490
/gene="URA8"
/locus_tag="YJR103W"
/EC_number="6.3.4.2"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0003883 CTP
synthase activity [PMID:7559626]"
/experiment="EXISTENCE:direct assay:GO:0006241 CTP
biosynthetic process [PMID:7559626]"
/experiment="EXISTENCE:direct assay:GO:0097268
cytoophidium [PMID:20713603]"
/experiment="EXISTENCE:mutant phenotype:GO:0003883 CTP
synthase activity [PMID:8121398]"
/note="Minor CTP synthase isozyme (see also URA7);
catalyzes the ATP-dependent transfer of the amide nitrogen
from glutamine to UTP, forming CTP, the final step in de
novo biosynthesis of pyrimidines; involved in phospholipid
biosynthesis; capable of forming cytoplasmic filaments
termed cytoophidium, especially during conditions of
glucose depletion; URA8 has a paralog, URA7, that arose
from the whole genome duplication"
/codon_start=1
/product="CTP synthase URA8"
/protein_id="NP_012637.4"
/db_xref="GeneID:853567"
/db_xref="SGD:S000003864"
/translation="MKYVVVSGGVISGIGKGVLASSTGMLLKTLGLKVTSIKIDPYMN
IDAGTMSPLEHGECFVLDDGGETDLDLGNYERYLGITLSRDHNITTGKIYSHVISRER
RGDYLGKTVQIVPHLTNAIQDWIQRVSKIPVDDTGLEPDVCIIELGGTVGDIESAPFV
EALRQFQFEVGRENFALIHVSLVPVIHGEQKTKPTQAAIKDLRSLGLIPDMIACRCSE
ELNRSTIDKIAMFCHVGPEQVVNVHDVNSTYHVPLLLLKQHMIDYLHSRLKLGEVPLT
LEDKERGSQLLTNWENMTKNLDDSDDVVKIALVGKYTNLKDSYLSVTKSLEHASMKCR
RQLEILWVEASNLEPETQEVDKNKFHDSWNKLSSADGILVPGGFGTRGIEGMILAAKW
ARESGVPFLGVCLGLQVAAIEFARNVIGRPNSSSTEFLDETLLAPEDQVVIYMPEIDK
EHMGGTMRLGLRPTIFQPNSEWSNIRKLYGEVNEVHERHRHRYEINPKIVNDMESRGF
IFVGKDETGQRCEIFELKGHPYYVGTQYHPEYTSKVLEPSRPFWGLVAAASGTLGEVI
KDINLSEGNENE"
gene complement(<622550..>623014)
/gene="SOD1"
/locus_tag="YJR104C"
/gene_synonym="CRS4"
/db_xref="GeneID:853568"
mRNA complement(<622550..>623014)
/gene="SOD1"
/locus_tag="YJR104C"
/gene_synonym="CRS4"
/product="superoxide dismutase SOD1"
/transcript_id="NM_001181762.1"
/db_xref="GeneID:853568"
CDS complement(622550..623014)
/gene="SOD1"
/locus_tag="YJR104C"
/gene_synonym="CRS4"
/EC_number="1.15.1.1"
/experiment="EXISTENCE:direct assay:GO:0004784 superoxide
dismutase activity [PMID:3290902]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:24647101|PMID:18977757]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005758
mitochondrial intermembrane space [PMID:11500508]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:36630448|PMID:11500508]"
/experiment="EXISTENCE:direct assay:GO:0016670
oxidoreductase activity, acting on a sulfur group of
donors, oxygen as acceptor [PMID:36630448]"
/experiment="EXISTENCE:mutant phenotype:GO:0006801
superoxide metabolic process [PMID:3290902]"
/experiment="EXISTENCE:mutant phenotype:GO:0006878
intracellular copper ion homeostasis [PMID:8530401]"
/experiment="EXISTENCE:mutant phenotype:GO:0006882
intracellular zinc ion homeostasis [PMID:11581253]"
/experiment="EXISTENCE:mutant phenotype:GO:0031505
fungal-type cell wall organization [PMID:20176017]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress [PMID:24647101]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:24647101]"
/experiment="EXISTENCE:mutant phenotype:GO:0050821 protein
stabilization [PMID:23332757]"
/experiment="EXISTENCE:mutant phenotype:GO:1901856
negative regulation of cellular respiration
[PMID:23332757]"
/experiment="EXISTENCE:mutant phenotype:GO:1990748
cellular detoxification [PMID:36630448]"
/experiment="EXISTENCE:physical interaction:GO:0050821
protein stabilization [PMID:23332757]"
/note="Cytosolic copper-zinc superoxide dismutase; also
sulfide oxidase; detoxifies superoxide and hydrogen
sulfide; stabilizes Yck1p and Yck2p kinases in glucose to
repress respiration; phosphorylated by Dun1p, enters
nucleus under oxidative stress to promote transcription of
stress response genes; abundance increases under DNA
replication stress; localization to mitochondrial
intermembrane space is modulated by MICOS complex; human
ortholog SOD1 implicated in ALS complements a null allele"
/codon_start=1
/product="superoxide dismutase SOD1"
/protein_id="NP_012638.1"
/db_xref="GeneID:853568"
/db_xref="SGD:S000003865"
/translation="MVQAVAVLKGDAGVSGVVKFEQASESEPTTVSYEIAGNSPNAER
GFHIHEFGDATNGCVSAGPHFNPFKKTHGAPTDEVRHVGDMGNVKTDENGVAKGSFKD
SLIKLIGPTSVVGRSVVIHAGQDDLGKGDTEESLKTGNAGPRPACGVIGLTN"
gene <623581..>624603
/gene="ADO1"
/locus_tag="YJR105W"
/db_xref="GeneID:853569"
mRNA <623581..>624603
/gene="ADO1"
/locus_tag="YJR105W"
/product="adenosine kinase"
/transcript_id="NM_001181763.1"
/db_xref="GeneID:853569"
CDS 623581..624603
/gene="ADO1"
/locus_tag="YJR105W"
/EC_number="2.7.1.20"
/experiment="EXISTENCE:direct assay:GO:0004001 adenosine
kinase activity [PMID:11223943]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0004001
adenosine kinase activity [PMID:11223943]"
/experiment="EXISTENCE:mutant phenotype:GO:0006144 purine
nucleobase metabolic process [PMID:11223943]"
/note="Adenosine kinase; required for the utilization of
S-adenosylmethionine (AdoMet); may be involved in
recycling adenosine produced through the methyl cycle"
/codon_start=1
/product="adenosine kinase"
/protein_id="NP_012639.1"
/db_xref="GeneID:853569"
/db_xref="SGD:S000003866"
/translation="MTAPLVVLGNPLLDFQADVTAEYLAKYSLKENDAILVDAKSGDA
KMAIFDELLQMPETKLVAGGAAQNTARGAAYVLGAGQVVYFGSVGKDKFSERLLNENE
KAGVKSMYQVQNDIGTGKCAALITGHNRSLVTDLGAANFFTPDHLDKHWDLVEAAKLF
YIGGFHLTVSPDAIVKLGQHAKENSKPFVLNFSAPFIPHVFKDALARVLPYATVIIAN
ESEAEAFCDAFQLDCANTDLEAIAQRIVKDSPVEKTVIFTHGVEPTVVVSSKGTSTYP
VKPLDSSKIVDTNGAGDAFAGGFMAGLTKGEDLETSIDMGQWLAALSIQEVGPSYPSE
KISYSK"
gene <624838..>627015
/gene="ECM27"
/locus_tag="YJR106W"
/db_xref="GeneID:853570"
mRNA <624838..>627015
/gene="ECM27"
/locus_tag="YJR106W"
/product="Ecm27p"
/transcript_id="NM_001181764.4"
/db_xref="GeneID:853570"
CDS 624838..627015
/gene="ECM27"
/locus_tag="YJR106W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0006874
intracellular calcium ion homeostasis [PMID:27242146]"
/experiment="EXISTENCE:mutant phenotype:GO:0070316
regulation of G0 to G1 transition [PMID:27242146]"
/note="Protein involved in calcium homeostasis and exit
from quiescence; required for proper trehalose levels
during quiescence; may play a role in cell wall
biosynthesis, mutants are hypersensitive to antifungal,
Papulacandin B; null mutants have increased plasmid loss;
interacts with Pdr5p"
/codon_start=1
/product="Ecm27p"
/protein_id="NP_012640.4"
/db_xref="GeneID:853570"
/db_xref="SGD:S000003867"
/translation="MDWAINVAHPRLLYKDPKLSVTFIVPSLFHIIIAFVLLGICASD
FLCPNVAHISDPNSLRSNGSLVSKTASHASHTGALMAVLLSWCNSSPDLFSNLMSWAT
STRETRSTSVSLSIGEVLGACGIILCIVEGSIFIIMSRTHIEISQIQKLSIMRDLLFS
LAAMCVMSYVSLMNQVTVLNCLLMAFLYAFYLVVKLTFKLNHSAETPDETAADTSLRE
NSVSPFLDDSLMASGLLPPIQPGFDISNSITHGIKPSLLSAMDFNSFLSMLENSSLEE
DDSRNEMAELNTLRSMTPGQHWSASATVAGEATSAGRPFSEPTNAFTEYRDSERAINS
SPAVFAPYRDNPDDEESQEQVLLETTTHGHFGAQEMRRFSKRSLGWIIKIFIPHLSNF
SQKSISDAIFSIITVPFFIIFKLSCPQPPSDILSYDPTLNRYSLTTLPIILLFIQSIT
APFLLCSILSVLLTYHLGYLVYLFPLILAMALILLLTAFITKVNLHNKFTLSLDSSNI
LQEKLQKRKLLERLNTSIQIIFLAIGIINIIIWISLLANSLIEMMEIYQKILGLSKAI
LGLTIFAWGNSVGDLISNISMCRLYKTQTHYQDRVRLATKFFMISCASCLGGVMLNSM
GGIGFSGLVSMLFIGAFNDNEWWFLRKVKLQETSQLDNTLNYKFIVSCVFIILQIILL
LLFFGGPNNIKRRLTKEMKLVGISMCGLWALATLINILLELFS"
gene <627340..>628326
/gene="LIH1"
/locus_tag="YJR107W"
/db_xref="GeneID:853571"
mRNA <627340..>628326
/gene="LIH1"
/locus_tag="YJR107W"
/product="putative lipase"
/transcript_id="NM_001181765.2"
/db_xref="GeneID:853571"
CDS 627340..628326
/gene="LIH1"
/locus_tag="YJR107W"
/EC_number="3.1.1.3"
/note="Putative lipase"
/codon_start=1
/product="putative lipase"
/protein_id="NP_012641.2"
/db_xref="GeneID:853571"
/db_xref="SGD:S000003868"
/translation="MPVVHCSSNLPITPYIYERLVYFIKASSISSCISDNLLLVNKTF
NDGGCPPHINFCNDEIINPTAGQTVVELVLNAKKGELGSGYLAVDHGKKVVILAFRGS
TTRQDWFSDFEIYPVNYSPLCVKEYRKLIEEGKIRECEGCKMHRGFLRFTETLGMDVF
KKMESILESFPEYRIVVTGHSLGAALASLAGIELKIRGFDPLVLTFATPKIFNSEMKQ
WVDELFETDAIEKESILKDEIQFRKGYFRVVHTGDYIPMVPPFYHPAGLEMFINKVGL
PQNAEDIEYRGKNNRLTLKDGFREGMSGLVEDWLHVYEHRAYFIDVVGCSGL"
gene complement(<628457..>628693)
/locus_tag="YJR107C-A"
/db_xref="GeneID:65052910"
mRNA complement(<628457..>628693)
/locus_tag="YJR107C-A"
/product="uncharacterized protein"
/transcript_id="NM_001395036.1"
/db_xref="GeneID:65052910"
CDS complement(628457..628693)
/locus_tag="YJR107C-A"
/experiment="EXISTENCE:mutant phenotype:GO:0071470
cellular response to osmotic stress [PMID:26554900]"
/note="hypothetical protein; encodes new type of domain,
which ab initio modeling suggests is predominantly
alpha-helical; nonessential for growth, deletion increases
sensitivity to osmostress; expressed at moderate to high
abundance; ORF also present in strains EC1118, YJM789,
RM11-1a, and AWRI1631; Gln24 in S288C reference is
substituted with Arg in wine strain EC1118; predicted
S-palmitoylation site on Cys2, suggesting membrane
association; transcript previously mischaracterized as
SUT646"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001381966.1"
/db_xref="GeneID:65052910"
/db_xref="SGD:S000303810"
/translation="MCDDSYDAVEEYYFNKSVAGISGQENWNKQLATQVYSRSLQPEI
LPTLKPLSCNKERANAGKRVSEEEQINGKRKRKD"
gene <628712..>629083
/gene="ABM1"
/locus_tag="YJR108W"
/db_xref="GeneID:853572"
mRNA <628712..>629083
/gene="ABM1"
/locus_tag="YJR108W"
/product="Abm1p"
/transcript_id="NM_001181766.1"
/db_xref="GeneID:853572"
CDS 628712..629083
/gene="ABM1"
/locus_tag="YJR108W"
/experiment="EXISTENCE:mutant phenotype:GO:0000226
microtubule cytoskeleton organization [PMID:10628851]"
/note="hypothetical protein; required for normal
microtubule organization"
/codon_start=1
/product="Abm1p"
/protein_id="NP_012642.1"
/db_xref="GeneID:853572"
/db_xref="SGD:S000003869"
/translation="MSWRYSILTVDGSFKIFIPWEIFLTWNFLSAAWLNSTESNTYIH
YSTCWGTSDYTLNISVIEATTEKLVDTRLLTTLENATAWINSNSIDEDEDDMPHATNV
ADRLDGLSLSKRVYSICHYEF"
gene complement(<629585..>632941)
/gene="CPA2"
/locus_tag="YJR109C"
/db_xref="GeneID:853573"
mRNA complement(<629585..>632941)
/gene="CPA2"
/locus_tag="YJR109C"
/product="carbamoyl-phosphate synthase
(glutamine-hydrolyzing) CPA2"
/transcript_id="NM_001181767.3"
/db_xref="GeneID:853573"
CDS complement(629585..632941)
/gene="CPA2"
/locus_tag="YJR109C"
/EC_number="6.3.5.5"
/EC_number="6.3.4.16"
/experiment="EXISTENCE:direct assay:GO:0004088
carbamoyl-phosphate synthase (glutamine-hydrolyzing)
activity [PMID:8626695]"
/experiment="EXISTENCE:direct assay:GO:0005951
carbamoyl-phosphate synthase complex [PMID:206535]"
/experiment="EXISTENCE:mutant phenotype:GO:0004088
carbamoyl-phosphate synthase (glutamine-hydrolyzing)
activity [PMID:6358221]"
/experiment="EXISTENCE:mutant phenotype:GO:0006526
L-arginine biosynthetic process [PMID:8626695]"
/note="Large subunit of carbamoyl phosphate synthetase;
carbamoyl phosphate synthetase catalyzes a step in the
synthesis of citrulline, an arginine precursor"
/codon_start=1
/product="carbamoyl-phosphate synthase
(glutamine-hydrolyzing) CPA2"
/protein_id="NP_012643.3"
/db_xref="GeneID:853573"
/db_xref="SGD:S000003870"
/translation="MTSIYTSTEPTNSAFTTEDYKPQLVEGVNSVLVIGSGGLSIGQA
GEFDYSGSQAIKALKEDNKFTILVNPNIATNQTSHSLADKIYYLPVTPEYITYIIELE
RPDAILLTFGGQTGLNCGVALDESGVLAKYNVKVLGTPIKTLITSEDRDLFASALKDI
NIPIAESFACETVDEALEAAERVKYPVIVRSAYALGGLGSGFANNASEMKELAAQSLS
LAPQILVEKSLKGWKEVEYEVVRDRVGNCITVCNMENFDPLGVHTGDSMVFAPSQTLS
DEEFHMLRSAAIKIIRHLGVIGECNVQYALQPDGLDYRVIEVNARLSRSSALASKATG
YPLAYTAAKIGLGYTLPELPNPITKTTVANFEPSLDYIVAKIPKWDLSKFQYVDRSIG
SSMKSVGEVMAIGRNYEEAFQKALRQVDPSLLGFQGSTEFGDQLDEALRTPTDRRVLA
IGQALIHENYTVERVNELSKIDKWFLYKCMNIVNIYKELESVKSLSDLSKDLLQRAKK
LGFSDKQIAVTINKHASTNINELEIRSLRKTLGIIPFVKRIDTLAAEFPAQTNYLYTT
YNATKNDVEFNENGMLVLGSGVYRIGSSVEFDWCAVNTAKTLRDQGKKTIMINYNPET
VSTDFDEVDRLYFEELSYERVMDIYELEQSEGCIISVGGQLPQNIALKLYDNGCNIMG
TNPNDIDRAENRHKFSSILDSIDVDQPEWSELTSVEEAKLFASKVNYPVLIRPSYVLS
GAAMSVVNNEEELKAKLTLASDVSPDHPVVMSKFIEGAQEIDVDAVAYNGNVLVHAIS
EHVENAGVHSGDASLVLPPQHLSDDVKIALKDIADKVAKAWKITGPFNMQIIKDGEHT
LKVIECNIRASRSFPFVSKVLGVNFIEIAVKAFLGGDIVPKPVDLMLNKKYDYVATKV
PQFSFTRLAGADPFLGVEMASTGEVASFGRDLIESYWTAIQSTMNFHVPLPPSGILFG
GDTSREYLGQVASIVATIGYRIYTTNETTKTYLQEHIKEKNAKVSLIKFPKNDKRKLR
ELFQEYDIKAVFNLASKRAESTDDVDYIMRRNAIDFAIPLFNEPQTALLFAKCLKAKI
AEKIKILESHDVIVPPEVRSWDEFIGFKAY"
gene <633615..>635681
/gene="YMR1"
/locus_tag="YJR110W"
/db_xref="GeneID:853574"
mRNA <633615..>635681
/gene="YMR1"
/locus_tag="YJR110W"
/product="phosphatidylinositol-3-phosphatase YMR1"
/transcript_id="NM_001181768.1"
/db_xref="GeneID:853574"
CDS 633615..635681
/gene="YMR1"
/locus_tag="YJR110W"
/EC_number="3.1.3.64"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:21124907]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0046856
phosphatidylinositol dephosphorylation [PMID:15169871]"
/experiment="EXISTENCE:mutant phenotype:GO:0004438
phosphatidylinositol-3-phosphate phosphatase activity
[PMID:15169871]"
/experiment="EXISTENCE:mutant phenotype:GO:0046856
phosphatidylinositol dephosphorylation [PMID:15169871]"
/note="Phosphatidylinositol 3-phosphate (PI3P)
phosphatase; involved in various protein sorting pathways,
including CVT targeting and endosome to vacuole transport;
has similarity to the conserved myotubularin dual
specificity phosphatase family"
/codon_start=1
/product="phosphatidylinositol-3-phosphatase YMR1"
/protein_id="NP_012644.1"
/db_xref="GeneID:853574"
/db_xref="SGD:S000003871"
/translation="MEYIKIAKVSNVVLHRRGTATQGTLHLTTHHLIFESPQLSTEFW
FPYPLIYGVHKNPGSTLLSKLTSTNQIQLEGTDSQNYKLYQGKDLWSFVNIKVIGKDY
AVFSLDFGGDLHLQARKVYDSILNLTVLSNITQLYAFIYISNNLERKLPSPDSWDIYD
PIKEFRRQGLDSKDETCPWRLSTVNEHYEFCPTYPSKLFVPRSTSDILLKHASKFRSQ
KRIPVLTYHHKATDCNILRSSQPLPGLINQRSIQDEKLVWESFNSFCNKDIRRTKHVI
VDARPRTNALAQMALGGGTENMDNYNFFLADNNMGVDKSLKLPTVTRLFLGIDNIHIV
SNTAAYMTEVICQGGDLNLPLEQNLIRSQKFSNWLKLNTLILKSVDMLLKSIIFNHSN
VLVHCSDGWDRTSQVVSLLEICLDPFYRTFEGFMILVEKDWCSFGHRFLERSGHLNSD
IRFHDNTMHSNFNDVDTNGDDLDIGVNTQDDYAEDDEGGEDETNLINLSRISKKFNEN
FKLNKKSLKFVSPVFQQFLDCVYQLLTQNPDLFEFNERFLRRLVYHLYSCQYGTFLSN
SEKEKFQQNLPNKTKSVWDYFRSRRKQFINPNFIQRKRSGMNEHDQNLEEEEKVEWIS
PDLKKVQWWWQLYGRKDSEMNDELRHKRDSVPISVDKKSKEHSNSDGGKGLNLSIFGF
DMFNRK"
gene complement(<635855..>636706)
/gene="PXP2"
/locus_tag="YJR111C"
/db_xref="GeneID:853575"
mRNA complement(<635855..>636706)
/gene="PXP2"
/locus_tag="YJR111C"
/product="Pxp2p"
/transcript_id="NM_001181769.1"
/db_xref="GeneID:853575"
CDS complement(635855..636706)
/gene="PXP2"
/locus_tag="YJR111C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:27392156]"
/note="Peroxisomal matrix protein with naturally active
promoter; well-conserved in fungi; localized to
peroxisomes under physiological growth conditions; levels
of some amino acids are altered upon both knockout and
overexpression, suggesting potential involvement of Pxp2p
in amino acid metabolism or related cellular metabolic
processes (needs further study); GFP-fusion protein
displays inherent dual localization with large proportion
localizing to cytosol"
/codon_start=1
/product="Pxp2p"
/protein_id="NP_012645.1"
/db_xref="GeneID:853575"
/db_xref="SGD:S000003872"
/translation="MNQILNAQRLIQLSQFHPKLKNIWYLVAAATFSVCNEPQEIPKL
YHYAMLLSNDNAHMYRFTLASQTIDLLRSELPMRKTLINENYQQPTFFQKQLTAKFRE
VILKTGPLAGLPRAINGLTMLKETTPDILVPHLDPIDPWEAAMGNSSPLSETSMRRKH
DKTIQERDHTIQNGLRHWNSIYNKVSTRVVNNLNSSYPDLWYYTLVHVYGPLFAFDEI
LSAQETSLVIIASLVPQDVNPQLRGHLKGALNIGCDKETVEAVRGLAILISQWCGVSW
KSGVVKL"
gene <637030..>637635
/gene="NNF1"
/locus_tag="YJR112W"
/db_xref="GeneID:853576"
mRNA <637030..>637635
/gene="NNF1"
/locus_tag="YJR112W"
/product="MIND complex subunit NNF1"
/transcript_id="NM_001181770.3"
/db_xref="GeneID:853576"
CDS 637030..637635
/gene="NNF1"
/locus_tag="YJR112W"
/experiment="EXISTENCE:direct assay:GO:0000444 MIS12/MIND
type complex [PMID:14633972]"
/experiment="EXISTENCE:direct assay:GO:0000776 kinetochore
[PMID:14657030]"
/experiment="EXISTENCE:direct assay:GO:0007059 chromosome
segregation [PMID:12455957]"
/note="Essential component of the outer kinetochore MIND
complex; joins kinetochore subunits contacting DNA to
those contacting microtubules; required for kinetochore
bi-orientation and accurate chromosome segregation;
complex consists of Mtw1p, Nnf1p, Nsl1p and Dsn1p;
homologous to metazoan CENP-H proteins"
/codon_start=1
/product="MIND complex subunit NNF1"
/protein_id="NP_012646.3"
/db_xref="GeneID:853576"
/db_xref="SGD:S000003873"
/translation="MVNSHGIRYIRLKQVFNRALDQSISKLQSWDKVSSCFPQYVNSK
QGAINVANCQRQLTEFWTELCQREFKEIMEERNVEQKLNELDELILEAKERYTDRDQD
EVNKGPAIDELSSKELVECHLYSQRMHAIHEIDERLAKVNEMNDQLAQELKDLETQVE
VEKNEIGKMYDEYLGSHTDQPANVLLVQSLNDMVLELKENY"
gene <637790..>638168
/gene="LLP1"
/locus_tag="YJR112W-A"
/db_xref="GeneID:1466470"
mRNA join(<637790..637809,637859..>638168)
/gene="LLP1"
/locus_tag="YJR112W-A"
/product="dolichol-linked oligosaccharide pyrophosphatase
LLP1"
/transcript_id="NM_001184523.1"
/db_xref="GeneID:1466470"
CDS join(637790..637809,637859..638168)
/gene="LLP1"
/locus_tag="YJR112W-A"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:41020759]"
/experiment="EXISTENCE:direct assay:GO:0009311
oligosaccharide metabolic process [PMID:41020759]"
/experiment="EXISTENCE:direct assay:GO:0016462
pyrophosphatase activity [PMID:41020759]"
/note="Dolichol-linked oligosaccharide (DLO)
pyrophosphatase; involved in homeostasis/quality control
of DLO by converting it into phosphorylated
oligosaccharides; mRNA translated through programmed +1
translational frameshifting via tRNA slippage; orthologs
encode members of VanZ family proteins, found in various
bacteria and fungi; likely to be localized to Golgi;
knock-out shows abnormal accumulation of DLO modified by
Golgi mannosyltransferases"
/codon_start=1
/product="dolichol-linked oligosaccharide pyrophosphatase
LLP1"
/protein_id="NP_878107.1"
/db_xref="GeneID:1466470"
/db_xref="SGD:S000028513"
/translation="MVQLRRTITTNKVFQAITSTNDKVAHFVVFMWESWLFVKMFAED
IVTFRKLQANKYVLGVLICSLCASVTSEFAQSVVSRGQRVFDVKDIICNFWGSLLGVG
IAFYQDR"
gene complement(<638232..>638975)
/gene="RSM7"
/locus_tag="YJR113C"
/gene_synonym="uS7m"
/db_xref="GeneID:853578"
mRNA complement(<638232..>638975)
/gene="RSM7"
/locus_tag="YJR113C"
/gene_synonym="uS7m"
/product="mitochondrial 37S ribosomal protein uS7m RSM7"
/transcript_id="NM_001181771.1"
/db_xref="GeneID:853578"
CDS complement(638232..638975)
/gene="RSM7"
/locus_tag="YJR113C"
/gene_synonym="uS7m"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:11278769]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:11278769]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005763
mitochondrial small ribosomal subunit [PMID:11278769]"
/note="Mitochondrial ribosomal protein of the small
subunit; has similarity to E. coli S7 ribosomal protein"
/codon_start=1
/product="mitochondrial 37S ribosomal protein uS7m RSM7"
/protein_id="NP_012647.1"
/db_xref="GeneID:853578"
/db_xref="SGD:S000003874"
/translation="MLHCARRYMLVRPRLLWQSGTCVARFQSSVRTPASEPSAEKGVD
EWLEAINELREEFSAKEYLPETSLAPPGQSKVDLLQGSQAGSKIKPTAEQLAQWEALK
SVPIPPRKNATLDHITNMIMRHGKKEKAQTILSRALYLVYCQTRQDPIQALEKSLDEL
APLMMTKTFNTGVAKASVIPVPLNKRQRNRIAWNWIVQSANQRVSSDFAVRLGEELTA
IAKGTSSAFEKRDQIHKTAIAHRAYIQLK"
gene <639942..>640451
/locus_tag="YJR115W"
/db_xref="GeneID:853579"
mRNA <639942..>640451
/locus_tag="YJR115W"
/product="uncharacterized protein"
/transcript_id="NM_001181773.3"
/db_xref="GeneID:853579"
CDS 639942..640451
/locus_tag="YJR115W"
/note="hypothetical protein; YJR115W has a paralog, ECM13,
that arose from the whole genome duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_012649.3"
/db_xref="GeneID:853579"
/db_xref="SGD:S000003876"
/translation="MFTNTRTILIYNSKVMNTHTHTHTHTHTHIYIYTGDQVSVRGRL
LSLKFFKVLKLFFPSPTSLATSHPPLSSMSPYMTIPQQYLYISKIRSKLSQCALTRHH
HRELDLRKMVGHANMLDRILDEIDEIDSEVVLCDAADGSSTAEAHSASPASSDSSPLT
NNIRPISIM"
gene <640825..>641664
/gene="TDA4"
/locus_tag="YJR116W"
/db_xref="GeneID:853580"
mRNA <640825..>641664
/gene="TDA4"
/locus_tag="YJR116W"
/product="Tda4p"
/transcript_id="NM_001181774.1"
/db_xref="GeneID:853580"
CDS 640825..641664
/gene="TDA4"
/locus_tag="YJR116W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/note="Protein distantly similar to ceramide synthases,
Lag1p and Lac1p; null mutant is sensitive to expression of
the top1-T722A allele; mutation confers sensitivity to
copper"
/codon_start=1
/product="Tda4p"
/protein_id="NP_012650.1"
/db_xref="GeneID:853580"
/db_xref="SGD:S000003877"
/translation="MNANSTTTAIGLTSPFEKLSFFPHSSNLILAHLHEIIFSFVFYQ
LAFSVVAPFLNKVVFRKHYTTIRDPLLKIDFNVHTVSMIQAVVSNTVLLPTLTTPMHY
NVVTYTDSYSSMVSSLSAGYFIWDLTMCVRYFKLYGLEFTGHAIGSVYVMLLSLRPFC
QPWIGRFLIYEASTPFVNINWFIMQCNAKSKNSIPLWFNVVNGLLLMTVFFVVRICWG
SIASALLFRQMWKVRDELPKFSAVTMMSLNIFMNLLNVLWFKKMIRIAKKLAKPAPTS
KLD"
gene <642007..>643368
/gene="STE24"
/locus_tag="YJR117W"
/gene_synonym="AFC1; PIO2"
/db_xref="GeneID:853581"
mRNA <642007..>643368
/gene="STE24"
/locus_tag="YJR117W"
/gene_synonym="AFC1; PIO2"
/product="zinc metalloprotease"
/transcript_id="NM_001181775.1"
/db_xref="GeneID:853581"
CDS 642007..643368
/gene="STE24"
/locus_tag="YJR117W"
/gene_synonym="AFC1; PIO2"
/EC_number="3.4.24.84"
/experiment="EXISTENCE:direct assay:GO:0004222
metalloendopeptidase activity
[PMID:11581258|PMID:27129777]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:16407407]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:11581258]"
/experiment="EXISTENCE:direct assay:GO:0007323 peptide
pheromone maturation [PMID:11581258]"
/experiment="EXISTENCE:direct assay:GO:0016485 protein
processing [PMID:27129777]"
/experiment="EXISTENCE:genetic interaction:GO:0007323
peptide pheromone maturation
[PMID:11581258|PMID:9065405|PMID:10825201]"
/experiment="EXISTENCE:genetic interaction:GO:0031204
post-translational protein targeting to membrane,
translocation [PMID:26771486]"
/experiment="EXISTENCE:genetic interaction:GO:0036503 ERAD
pathway [PMID:26771486]"
/experiment="EXISTENCE:genetic interaction:GO:0071586
CAAX-box protein processing [PMID:9065405]"
/experiment="EXISTENCE:mutant phenotype:GO:0005637 nuclear
inner membrane [PMID:18923140]"
/experiment="EXISTENCE:mutant phenotype:GO:0007323 peptide
pheromone maturation [PMID:10825201|PMID:9065405]"
/experiment="EXISTENCE:mutant phenotype:GO:0031204
post-translational protein targeting to membrane,
translocation [PMID:26771486]"
/experiment="EXISTENCE:mutant phenotype:GO:0036503 ERAD
pathway [PMID:26771486]"
/experiment="EXISTENCE:mutant phenotype:GO:0071586
CAAX-box protein processing [PMID:9065405]"
/experiment="EXISTENCE:mutant phenotype:GO:0120236
negative regulation of post-translational protein
targeting to membrane, translocation [PMID:32513868]"
/note="Highly conserved zinc metalloprotease; component of
the ER quality control mechanism that removes faulty
proteins clogging translocation channels; inhibits
SRP-independent translocation into the ER; has two roles
in a-factor maturation, C-terminal CAAX proteolysis and
the first step of N-terminal proteolytic processing;
cleaves isoprenylated and non-prenylated oligopeptides;
human homolog ZMPSTE24 implicated in mandibuloacral
dysplasia (MAD), and complements the null mutant"
/codon_start=1
/product="zinc metalloprotease"
/protein_id="NP_012651.1"
/db_xref="GeneID:853581"
/db_xref="SGD:S000003878"
/translation="MFDLKTILDHPNIPWKLIISGFSIAQFSFESYLTYRQYQKLSET
KLPPVLEDEIDDETFHKSRNYSRAKAKFSIFGDVYNLAQKLVFIKYDLFPKIWHMAVS
LLNAVLPVRFHMVSTVAQSLCFLGLLSSLSTLVDLPLSYYSHFVLEEKFGFNKLTVQL
WITDMIKSLTLAYAIGGPILYLFLKIFDKFPTDFLWYIMVFLFVVQILAMTIIPVFIM
PMFNKFTPLEDGELKKSIESLADRVGFPLDKIFVIDGSKRSSHSNAYFTGLPFTSKRI
VLFDTLVNSNSTDEITAVLAHEIGHWQKNHIVNMVIFSQLHTFLIFSLFTSIYRNTSF
YNTFGFFLEKSTGSFVDPVITKEFPIIIGFMLFNDLLTPLECAMQFVMSLISRTHEYQ
ADAYAKKLGYKQNLCRALIDLQIKNLSTMNVDPLYSSYHYSHPTLAERLTALDYVSEK
KKN"
gene complement(<643490..>644101)
/gene="ILM1"
/locus_tag="YJR118C"
/db_xref="GeneID:853582"
mRNA complement(<643490..>644101)
/gene="ILM1"
/locus_tag="YJR118C"
/product="Ilm1p"
/transcript_id="NM_001181776.3"
/db_xref="GeneID:853582"
CDS complement(643490..644101)
/gene="ILM1"
/locus_tag="YJR118C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095]"
/note="hypothetical protein; may be involved in
mitochondrial DNA maintenance; required for slowed DNA
synthesis-induced filamentous growth"
/codon_start=1
/product="Ilm1p"
/protein_id="NP_012652.3"
/db_xref="GeneID:853582"
/db_xref="SGD:S000003879"
/translation="MAQALNSTNIAFFRVAFLFTIAFFCLKNVNSILQNTYFIVLTQA
MNLPQLTLSRYSGQLGLFALLFTLNGVHDLIPLLENNVKYFQSVVPVRLLIFFILTSI
SYLWESNFYVHNNSVFIYCFAEVWINFLLYNAIREEKNEEFKRLNQFMVNDEDIEEPQ
PFTVKTETTEIIEIINDEENDDEDGKDNDDNNEKGNDDSDAKK"
gene complement(<644304..>646490)
/gene="JHD2"
/locus_tag="YJR119C"
/gene_synonym="KDM5"
/db_xref="GeneID:853583"
mRNA complement(<644304..>646490)
/gene="JHD2"
/locus_tag="YJR119C"
/gene_synonym="KDM5"
/product="histone demethylase"
/transcript_id="NM_001181777.1"
/db_xref="GeneID:853583"
CDS complement(644304..646490)
/gene="JHD2"
/locus_tag="YJR119C"
/gene_synonym="KDM5"
/EC_number="1.14.11.67"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0032453 histone
H3K4 demethylase activity [PMID:17310254]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:27325136]"
/experiment="EXISTENCE:mutant phenotype:GO:0000183 rDNA
heterochromatin formation [PMID:25248920]"
/experiment="EXISTENCE:mutant phenotype:GO:0000278 mitotic
cell cycle [PMID:25248920]"
/experiment="EXISTENCE:mutant phenotype:GO:0032453 histone
H3K4 demethylase activity [PMID:17310254|PMID:17369256]"
/experiment="EXISTENCE:mutant phenotype:GO:0043934
sporulation [PMID:23123093]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:27325136|PMID:23123093]"
/experiment="EXISTENCE:mutant phenotype:GO:0060623
regulation of chromosome condensation [PMID:25248920]"
/experiment="EXISTENCE:mutant phenotype:GO:0071041
antisense RNA transcript catabolic process
[PMID:23123093]"
/experiment="EXISTENCE:mutant phenotype:GO:1902275
regulation of chromatin organization [PMID:27325136]"
/note="JmjC domain family histone demethylase; promotes
global demethylation of H3K4 and represses noncoding
intergenic transcription during sporulation; removes
methyl groups added by Set1p; phosphorylation by Tpk2p
during glycolysis inhibits activity, nuclear localization,
chromatin binding and promotes Not4p Ub-mediated
degradation; negatively regulated by H3K14 acetylation;
regulates sporulation timing by extending period of active
transcription; regulates rDNA silencing; human homolog is
JARID1C"
/codon_start=1
/product="histone demethylase"
/protein_id="NP_012653.1"
/db_xref="GeneID:853583"
/db_xref="SGD:S000003880"
/translation="MEEIPALYPTEQEFKNPIDYLSNPHIKRLGVRYGMVKVVPPNGF
CPPLSIDMENFTFQPRIQNLENLDLKNRCRLFFMKQLNNFKRSVKDPSKLILREPYTI
VEYSDSTHASEILKKKVYFYDVFSELIKDNRTLTDTTQSFRRKLKFRDISQLRGDISL
WRTISKKFNVPIGLLKEIFEKYIASYYIFLHSLNENVHTALHADQYPKSLLSDDEDDF
DLGPDSNSGSDFEEDDDDACIVCRKTNDPKRTILCDSCDKPFHIYCLSPPLERVPSGD
WICNTCIVGNGYYGFTQDTHDYSLPEFQEYCKRQNSRLLPARKLSIDELEEMFWSLVT
KNRRSSLTTVKYGADIHNELPGQITGFPTREFIPKNINGDELIDYLKYCDHPMNLTNL
PMAHNSLLPLFKRNISGMTIPWIYIGSLFSTFCWHMEDQYTLSANYQHEGDPKVWYSI
PESGCTKFNDLLNDMSPDLFIKQPDLLHQLVTLISPYDPNFKKSGIPVYKAVQKPNEY
IITFPKCYHAGFNTGYNFNEAVNFTIDFWLPYGFGAITDYKLTQKACVFDMFDLMINV
LDKYNKDTLLFNDAFVRQCYSSLIVFYNTELKRIRKIQAIVPRTTLLEVHTDPNDEDE
EYDIFCSQCKTICSIAFVLRKNNYDSIRTYKRHKKNHLSIRQWNELSTTDSKVSILCT
QDYLKSIQNLNNSDGEEPYIDDELYFTKSLKDIDSLIKQVGVKLDR"
gene <647126..>647476
/gene="DMO1"
/locus_tag="YJR120W"
/db_xref="GeneID:853584"
mRNA <647126..>647476
/gene="DMO1"
/locus_tag="YJR120W"
/product="Dmo1p"
/transcript_id="NM_001181778.1"
/db_xref="GeneID:853584"
CDS 647126..647476
/gene="DMO1"
/locus_tag="YJR120W"
/experiment="EXISTENCE:mutant phenotype:GO:0007005
mitochondrion organization [PMID:10628851]"
/experiment="EXISTENCE:mutant phenotype:GO:0015918 sterol
transport [PMID:16251356]"
/experiment="EXISTENCE:mutant phenotype:GO:0045333
cellular respiration [PMID:10628851]"
/note="hypothetical protein; essential for growth under
anaerobic conditions; mutation causes decreased expression
of ATP2, impaired respiration, defective sterol uptake,
and altered levels/localization of ABC transporters Aus1p
and Pdr11p"
/codon_start=1
/product="Dmo1p"
/protein_id="NP_012654.1"
/db_xref="GeneID:853584"
/db_xref="SGD:S000003881"
/translation="MRWDVIILYAISRPYATRRTGSHTHPRDSRYIAANQRRPPSACR
VGPSPAKQRKDIPIFELLDTTLIKNALFALTSFLYYRTNILTCPFLNFLYLSRTGQLD
KFCKDQTVTQILAT"
gene <647607..>649142
/gene="ATP2"
/locus_tag="YJR121W"
/db_xref="GeneID:853585"
mRNA <647607..>649142
/gene="ATP2"
/locus_tag="YJR121W"
/product="F1F0 ATP synthase subunit beta"
/transcript_id="NM_001181779.3"
/db_xref="GeneID:853585"
CDS 647607..649142
/gene="ATP2"
/locus_tag="YJR121W"
/EC_number="7.1.2.2"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0015986 proton
motive force-driven ATP synthesis [PMID:20691145]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:18722382|PMID:15294286]"
/experiment="EXISTENCE:direct assay:GO:0045259
proton-transporting ATP synthase complex
[PMID:15294286|PMID:17082766]"
/experiment="EXISTENCE:direct assay:GO:0046933
proton-transporting ATP synthase activity, rotational
mechanism [PMID:20691145]"
/experiment="EXISTENCE:mutant phenotype:GO:0015986 proton
motive force-driven ATP synthesis
[PMID:2529856|PMID:6225776]"
/experiment="EXISTENCE:mutant phenotype:GO:0045259
proton-transporting ATP synthase complex [PMID:6225776]"
/experiment="EXISTENCE:mutant phenotype:GO:0046933
proton-transporting ATP synthase activity, rotational
mechanism [PMID:6225776]"
/experiment="EXISTENCE:mutant phenotype:GO:0046961
proton-transporting ATPase activity, rotational mechanism
[PMID:2529856]"
/note="Beta subunit of the F1 sector of mitochondrial F1F0
ATP synthase; which is a large, evolutionarily conserved
enzyme complex required for ATP synthesis; F1
translationally regulates ATP6 and ATP8 expression to
achieve a balanced output of ATP synthase genes encoded in
nucleus and mitochondria; phosphorylated"
/codon_start=1
/product="F1F0 ATP synthase subunit beta"
/protein_id="NP_012655.3"
/db_xref="GeneID:853585"
/db_xref="SGD:S000003882"
/translation="MVLPRLYTATSRAAFKAAKQSAPLLSTSWKRCMASAAQSTPITG
KVTAVIGAIVDVHFEQSELPAILNALEIKTPQGKLVLEVAQHLGENTVRTIAMDGTEG
LVRGEKVLDTGGPISVPVGRETLGRIINVIGEPIDERGPIKSKLRKPIHADPPSFAEQ
STSAEILETGIKVVDLLAPYARGGKIGLFGGAGVGKTVFIQELINNIAKAHGGFSVFT
GVGERTREGNDLYREMKETGVINLEGESKVALVFGQMNEPPGARARVALTGLTIAEYF
RDEEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGLLQERITTTKK
GSVTSVQAVYVPADDLTDPAPATTFAHLDATTVLSRGISELGIYPAVDPLDSKSRLLD
AAVVGQEHYDVASKVQETLQTYKSLQDIIAILGMDELSEQDKLTVERARKIQRFLSQP
FAVAEVFTGIPGKLVRLKDTVASFKAVLEGKYDNIPEHAFYMVGGIEDVVAKAEKLAA
EAN"
gene <649776..>651269
/gene="IBA57"
/locus_tag="YJR122W"
/gene_synonym="CAF17"
/db_xref="GeneID:853586"
mRNA <649776..>651269
/gene="IBA57"
/locus_tag="YJR122W"
/gene_synonym="CAF17"
/product="Iba57p"
/transcript_id="NM_001181780.1"
/db_xref="GeneID:853586"
CDS 649776..651269
/gene="IBA57"
/locus_tag="YJR122W"
/gene_synonym="CAF17"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:18086897]"
/experiment="EXISTENCE:mutant phenotype:GO:0051604 protein
maturation [PMID:18086897|PMID:29343425]"
/note="Protein involved in incorporating iron-sulfur
clusters into proteins; mitochondrial matrix protein;
involved in the incorporation of iron-sulfur clusters into
mitochondrial aconitase-type proteins; activates the
radical-SAM family members Bio2p and Lip5p; interacts with
Ccr4p in the two-hybrid system"
/codon_start=1
/product="Iba57p"
/protein_id="NP_012656.1"
/db_xref="GeneID:853586"
/db_xref="SGD:S000003883"
/translation="MFISRRCRIKGFTLKNLLWFRSSSTRFVSTESPDASAITKPDGI
FNYSLLENRTYIRIRGPDTVKFLNGLVTSKLLPHFIKKNLTTVEENEVPTEEGTTKVD
PIIPVPEFDARLGNWGLYNEKGIQGPYISRFGLYSAFLNGKGKLITDTIIYPTPVTVS
EQISNYPEYLLELHGNVVDKILHVLQTHKLANKIKFEKIDHSSLKTWDVEVQFPNLPK
DIENPWFDNLLDPMALPKNSIDANNFAVNVLNSLFNSDPRILGIYVERRTESMSRHYS
TFPQSFRVVTSEQVDDLSKLFNFNVFDFPFQVNKKASVQVREIRFQKGLIDSTEDYIS
ETLLPLELNFDFFPNTISTNKGCYVGQELTARTYATGILRKRLVPVKLDNYQLLDTDP
ERKYAEFHIDNVVEKSLAENEPTLNPFTNKPPERTKRKQRPAGLLISNEGLYGVALLR
TEHFSAAFSSDEPVEFYITTTKGENIKITPQKPFWFSDWKNNNGPHK"
gene <651901..>652578
/gene="RPS5"
/locus_tag="YJR123W"
/db_xref="GeneID:853587"
mRNA <651901..>652578
/gene="RPS5"
/locus_tag="YJR123W"
/product="40S ribosomal protein uS7 RPS5"
/transcript_id="NM_001181781.1"
/db_xref="GeneID:853587"
CDS 651901..652578
/gene="RPS5"
/locus_tag="YJR123W"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:6814480]"
/experiment="EXISTENCE:direct assay:GO:0022627 cytosolic
small ribosomal subunit [PMID:6814480|PMID:385049]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/experiment="EXISTENCE:mutant phenotype:GO:0000054
ribosomal subunit export from nucleus [PMID:16246728]"
/experiment="EXISTENCE:mutant phenotype:GO:0030490
maturation of SSU-rRNA [PMID:20419091]"
/note="Protein component of the small (40S) ribosomal
subunit; least basic of non-acidic ribosomal proteins;
phosphorylated in vivo; essential for viability;
homologous to mammalian ribosomal protein S5 and bacterial
S7"
/codon_start=1
/product="40S ribosomal protein uS7 RPS5"
/protein_id="NP_012657.1"
/db_xref="GeneID:853587"
/db_xref="SGD:S000003884"
/translation="MSDTEAPVEVQEDFEVVEEFTPVVLATPIPEEVQQAQTEIKLFN
KWSFEEVEVKDASLVDYVQVRQPIFVAHTAGRYANKRFRKAQCPIIERLTNSLMMNGR
NNGKKLKAVRIIKHTLDIINVLTDQNPIQVVVDAITNTGPREDTTRVGGGGAARRQAV
DVSPLRRVNQAIALLTIGAREAAFRNIKTIAETLAEELINAAKGSSTSYAIKKKDELE
RVAKSNR"
gene complement(<652892..>654238)
/locus_tag="YJR124C"
/db_xref="GeneID:853588"
mRNA complement(<652892..>654238)
/locus_tag="YJR124C"
/product="uncharacterized protein"
/transcript_id="NM_001181782.3"
/db_xref="GeneID:853588"
CDS complement(652892..654238)
/locus_tag="YJR124C"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:19001347|PMID:26928762]"
/note="hypothetical protein; expression induced under
calcium shortage"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_012658.3"
/db_xref="GeneID:853588"
/db_xref="SGD:S000003885"
/translation="MAPEIFVKFKCASRDIKLLWASVFLRLLSYGLTNQVLTLFLNAI
NMTEDKIGLFMSLTLAGDVICSYILTWYADSWGRRRVLVYGCAMMLLSGLVFSFSENF
TLLLVFAIFGVISPSSDEVGPFKSIEEAMIAHLSPHNARPEIYAIHALVGTIGSALGA
IICGIFVDLLKRTGLAATDLQCYKLVFLLYAFFAFCKMVIMLLLSDATELDGHYEHTD
CNEETAEPLDVNDETAPLMRQATHPEERSNKLSKETVSVLMKLLVIFMVDSLGSGFMT
SGWMVYYYSKQFLMGSLALGTLFFITQLVMASSTIPSSIIARCFGPVRATLLVQIPSG
IFSILIPMAKNYLPLSILFLNLHFATTAMDVTPRQILLTNIIKPRDLTKVMGVVNIGK
TFARCVGPIFTGILANNNYLWLCYIISGSLVITADLILACMFLGVDAKIKKQMNRH"
rep_origin 654375..654614
/note="ARS1020; Autonomously Replicating Sequence"
/db_xref="SGD:S000028457"
gene complement(<654737..>655963)
/gene="ENT3"
/locus_tag="YJR125C"
/db_xref="GeneID:853589"
mRNA complement(<654737..>655963)
/gene="ENT3"
/locus_tag="YJR125C"
/product="Ent3p"
/transcript_id="NM_001181783.1"
/db_xref="GeneID:853589"
CDS complement(654737..655963)
/gene="ENT3"
/locus_tag="YJR125C"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:12967568]"
/experiment="EXISTENCE:direct assay:GO:0030125 clathrin
vesicle coat [PMID:12483220]"
/experiment="EXISTENCE:direct assay:GO:0030276 clathrin
binding [PMID:12967568]"
/experiment="EXISTENCE:direct assay:GO:0032266
phosphatidylinositol-3-phosphate binding [PMID:14630930]"
/experiment="EXISTENCE:direct assay:GO:0080025
phosphatidylinositol-3,5-bisphosphate binding
[PMID:14630930]"
/experiment="EXISTENCE:genetic interaction:GO:0034498
early endosome to Golgi transport [PMID:20658963]"
/experiment="EXISTENCE:mutant phenotype:GO:0006895 Golgi
to endosome transport [PMID:12483220]"
/experiment="EXISTENCE:mutant phenotype:GO:0030036 actin
cytoskeleton organization [PMID:12967568]"
/experiment="EXISTENCE:mutant phenotype:GO:0032511 late
endosome to vacuole transport via multivesicular body
sorting pathway [PMID:15107463]"
/experiment="EXISTENCE:mutant phenotype:GO:0071985
multivesicular body sorting pathway [PMID:12967568]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:12483220]"
/note="Protein containing an N-terminal epsin-like domain;
involved in clathrin recruitment and traffic between the
Golgi and endosomes; associates with the clathrin adaptor
Gga2p"
/codon_start=1
/product="Ent3p"
/protein_id="NP_012659.1"
/db_xref="GeneID:853589"
/db_xref="SGD:S000003886"
/translation="MSLEDTLANMSLYDAKKYFRKAQNVVFNYTEMEGKVREATNNEP
WGASSTLMDQISQGTYNFREREEILSMIFRRFTEKAGSEWRQIYKALQLLDYLIKHGS
ERFIDDTRNSINLIRILETFHYIDSQGRDQGINVRTRVKALIELLSDDNKIRAERKKA
RETAKKYKGVAGGSASADGSLNSKAGFTSTKVHGISVSADFDSDNEDNEDGSFSQNGY
NDNASRATSTPGQGKQEPEDFVDFFSSESSKPSKELIQEDEKKADEEEDDDDEFSEFQ
SAVPVTNPANSFNLLNTSPIEGMPATTSSMPFYNSSTTDQGKITPAIAEPKKVDPFSS
LFSTAKASAEAPSAPKASQAKAAASNPVSNSTTALSTDQDDDDEFGEMHGGAVQQEQN
TNNNHTSSKEIDLLSF"
gene complement(<656254..>658689)
/gene="VPS70"
/locus_tag="YJR126C"
/db_xref="GeneID:853590"
mRNA complement(<656254..>658689)
/gene="VPS70"
/locus_tag="YJR126C"
/product="putative zinc metalloprotease"
/transcript_id="NM_001181784.1"
/db_xref="GeneID:853590"
CDS complement(656254..658689)
/gene="VPS70"
/locus_tag="YJR126C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0006623 protein
targeting to vacuole [PMID:12134085]"
/note="hypothetical protein involved in vacuolar protein
sorting; regulates intra-species translation fidelity and
lifespan in a vacuolar-dependent manner; SWAT-GFP and
mCherry fusion proteins localize to the endoplasmic
reticulum"
/codon_start=1
/product="putative zinc metalloprotease"
/protein_id="NP_012660.1"
/db_xref="GeneID:853590"
/db_xref="SGD:S000003887"
/translation="MRMIQRERKREKEEGQLKERTVVNMADPDDNEAEATGLQQYSGE
TTRDDNEESMNDSFTLTSRNRGRSNTISSIVSGYEIMKEHMDKEKFMYLILASLLLYM
GFVAAFAPRTSLSRDFRRFHSSRLTNAEVYRIYLNSLQQENRAKEHVYKYAGYMSNGA
SDSSTFKYTLDEFLDMGYKPKVEKYYPWIGEPVDTNVAPLENGKVVYEASMIEDRVKG
DPASHARKRQKGFHQYSKNGSVTARYVFCNYGSISDYKLLLKKNIDIEDKIHIVRSGK
ILPGLKVKNAELYGASSVIIYTDPFDDGKVTEENGFLHYPYGPARNPSYIRRDSVNYF
SDTPGDPTTPGYPSKDSDTEHMSPVGRVPRIPSVPMSARDVQPILERLNGRGFQIGPG
SNIKDFGSFTGPSSSIDKVHLHNELTYNIKEMSSVEVSIPGIFTEGEIIIGAHRDSLA
SSSAGDANSGSAILLEIARGMSKLLKHGWKPLRPIKLISWDGERSGLLGSTDYAEAHA
AILRRRALVYLNLDNAISGTNFHCKANPLLQDVIYEAAKLTEFNGHEDWSLFDHWKYT
SNATISLLDGLSSYTSFQYHLGVPAAHFQFNANDTSGAVYHSNSVFDSPTWLEKFTNS
DYKLHNTMAMFVGLTTLMLSENELARFNTHVYLKKIYNWYIAWHSNLSSAFPQDDEVN
SLAKRVLDLLKVATQEDSIQFDQQNGILYKECREALPVWAFYKKIKSYIKLQRSNSKS
KQIDQLFITHRGLKDREWMKYSLLAPSKFEGSVGEVLPGLHEGLADIDRNEVIQWLTI
LLSQFSNVRYLLQ"
gene complement(<658917..>663059)
/gene="RSF2"
/locus_tag="YJR127C"
/gene_synonym="ZMS1"
/db_xref="GeneID:853592"
mRNA complement(<658917..>663059)
/gene="RSF2"
/locus_tag="YJR127C"
/gene_synonym="ZMS1"
/product="Rsf2p"
/transcript_id="NM_001181785.3"
/db_xref="GeneID:853592"
CDS complement(658917..663059)
/gene="RSF2"
/locus_tag="YJR127C"
/gene_synonym="ZMS1"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:19111667]"
/experiment="EXISTENCE:mutant phenotype:GO:0006355
regulation of DNA-templated transcription [PMID:16208474]"
/note="Zinc-finger protein; involved in transcriptional
control of both nuclear and mitochondrial genes, many of
which specify products required for glycerol-based growth,
respiration, and other functions; RSF2 has a paralog,
TDA9, that arose from the whole genome duplication;
relocalizes from nucleus to cytoplasm upon DNA replication
stress"
/codon_start=1
/product="Rsf2p"
/protein_id="NP_012661.3"
/db_xref="GeneID:853592"
/db_xref="SGD:S000003888"
/translation="MEPFAFGRGAPALCILTAAARINLDNFVPCCWALFRLSFFFPLD
PAYIRNENKETRTSWISIEFFFFVKHCLSQHTFFSKTLAPKRNFRAKKLKDIGDTRID
RADKDFLLVPEPSMFVNGNQSNFAKPAGQGILPIPKKSRIIKTDKPRPFLCPTCTRGF
VRQEHLKRHQHSHTREKPYLCIFCGRCFARRDLVLRHQQKLHAALVGTGDPRRMTPAP
NSTSSFASKRRHSVAADDPTDLHIIKIAGNKETILPTPKNLAGKTSEELKEAVVALAK
SNNVELPVSAPVMNDKREKTPPSKAGSLGFREFKFSTKGVPVHSASSDAVIDRANTPS
SMHKTKRHASFSASSAMTYMSSSNSPHHSITNFELVEDAPHQVGFSTPQMTAKQLMES
VSELDLPPLTLDEPPQAIKFNLNLFNNDPSGQQQQQQQQQQNSTSSTIVNSNNGSTVA
TPGVYLLSSGPSLTDLLTMNSAHAGAGGYMSSHHSPFDLGCFSHDKPTVSEFNLPSSF
PNTIPSNSTTASNSYSNLANQTYRQMSNEQPLMSLSPKNPPTTVSDSSSTINFNPGTN
NLLEPSMEPNDKDSNIDPAAIDDKWLSEFINNSDPKSTFKINFNHFNDIGFIYSPPSS
RSSIPNKSPPNHSATSLNHEKASLSPRLNLSLNGSTDLPSTPQNQLKEPSYSDPISHS
SHKRRRDSVMMDYDLSNFFSSRQLDISKVLNGTEQNNSHVNDDVLTLSFPGETDSNAT
QKQLPVLTPSDLLSPFSVPSVSQVLFTNELRSMMLADNNIDSGAFPTTSQLNDYVTYY
KEEFHPFFSFIHLPSIIPNMDSYPLLLSISMVGALYGFHSTHAKVLANAASTQIRKSL
KVSEKNPETTELWVIQTLVLLTFYCIFNKNTAVIKGMHGQLTTIIRLLKASRLNLPLE
SLCQPPIESDHIMEYENSPHMFSKIREQYNAPNQMNKNYQYFVLAQSRIRTCHAVLLI
SNLFSSLVGADCCFHSVDLKCGVPCYKEELYQCRNSDEWSDLLCQYKITLDSKFSLIE
LSNGNEAYENCLRFLSTGDSFFYGNARVSLSTCLSLLISIHEKILIERNNARISNNNT
NSNNIELDDIEWKMTSRQRIDTMLKYWENLYLKNGGILTPTENSMSTINANPAMRLII
PVYLFAKMRRCLDLAHVIEKIWLKDWSNMNKALEEVCYDMGSLREATEYALNMVDAWT
SFFTYIKQGKRRIFNTPVFATTCMFTAVLVISEYMKCVEDWARGYNANNPNSALLDFS
DRVLWLKAERILRRLQMNLIPKECDVLKSYTDFLRWQDKDALDLSALNEEQAQRAMDP
NTDINETIQLIVAASLSSKCLYLGVQILGDAPIWPIILSFAHGLQSRAIYSVTKKRNT
RI"
gene <662921..>663280
/locus_tag="YJR128W"
/db_xref="GeneID:853591"
mRNA <662921..>663280
/locus_tag="YJR128W"
/product="uncharacterized protein"
/transcript_id="NM_001348848.1"
/db_xref="GeneID:853591"
CDS 662921..663280
/locus_tag="YJR128W"
/note="hypothetical protein; conserved among S. cerevisiae
strains; partially overlaps the verified ORF RSF2"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335788.1"
/db_xref="GeneID:853591"
/db_xref="SGD:S000003889"
/translation="MQGQAGKRKTDGKVPSNTEQNCPDLFERPRLVCIMQAPLVQMRT
VPSETGHFPKIISLNRSCSRSYLLSMPHTQLPYPLPYGEFPRCSTVRRDHNFPCTLYS
APGVVHPRLCVFSCMMI"
gene 663749..663942
/gene="SNR3"
/locus_tag="YNCJ0030W"
/db_xref="GeneID:9164912"
ncRNA 663749..663942
/ncRNA_class="snoRNA"
/gene="SNR3"
/locus_tag="YNCJ0030W"
/product="SNR3"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:3327689]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:3327689]"
/experiment="EXISTENCE:mutant phenotype:GO:0000454 snoRNA
guided rRNA pseudouridine synthesis
[PMID:15923376|PMID:9160748|PMID:15306656]"
/experiment="EXISTENCE:mutant phenotype:GO:0030559 rRNA
pseudouridylation guide activity
[PMID:15923376|PMID:9160748|PMID:15306656]"
/experiment="EXISTENCE:physical interaction:GO:0003723 RNA
binding [PMID:3327689]"
/experiment="EXISTENCE:physical interaction:GO:0031429 box
H/ACA snoRNP complex [PMID:15923376|PMID:9472021]"
/note="H/ACA box small nucleolar RNA (snoRNA); guides
pseudouridylation of large subunit (LSU) rRNA at positions
U2129, U2133, and U2264"
/transcript_id="NR_132198.1"
/db_xref="GeneID:9164912"
/db_xref="SGD:S000006492"
gene complement(<664000..>665019)
/gene="EFM3"
/locus_tag="YJR129C"
/db_xref="GeneID:853593"
mRNA complement(<664000..>665019)
/gene="EFM3"
/locus_tag="YJR129C"
/product="protein-lysine N-methyltransferase"
/transcript_id="NM_001181787.2"
/db_xref="GeneID:853593"
CDS complement(664000..665019)
/gene="EFM3"
/locus_tag="YJR129C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0016279
protein-lysine N-methyltransferase activity
[PMID:25086354]"
/experiment="EXISTENCE:direct assay:GO:0018023
peptidyl-lysine trimethylation [PMID:25086354]"
/experiment="EXISTENCE:mutant phenotype:GO:0018023
peptidyl-lysine trimethylation
[PMID:25086354|PMID:25231983]"
/note="S-adenosylmethionine-dependent methyltransferase;
seven-beta-strand lysine methyltransferase which
trimethylates translation elongation factor EF2 (Eft1p and
Eft2p) at lysine 509; green fluorescent protein
(GFP)-fusion protein localizes to the cytoplasm; ortholog
of human gene FAM86A"
/codon_start=1
/product="protein-lysine N-methyltransferase"
/protein_id="NP_012663.2"
/db_xref="GeneID:853593"
/db_xref="SGD:S000003890"
/translation="MNEDLFYDRLHQRCPGKYLLEELETSKSNDVLHASRFVCEMELV
QKTNAYYCKTIVKMLLDHEWIFAKAFTIVNDGEDEIEIYDYLYEKYIKLLSTGKPDPM
MKDVVRYRFDEDVKIKIEETPNLISAASTTGFRTWEAALYMGDFLIHKPLQELAPVQG
QDDGKKKLNVLEVGAGTGIVSLVILQKYHEFVNKMYVTDGDSNLVETQLKRNFELNNE
VRENEPDIKLQRLWWGSDRVPEDIDLVVGADVTYDPTILPDLCECLAECLALDRCKLC
LLSATIRSESTVQLFSQECNKLGLKCTIVTSTEYDANNEIRAMKALQFKPLIAPIRIY
KITKQ"
gene complement(<665218..>667137)
/gene="STR2"
/locus_tag="YJR130C"
/db_xref="GeneID:853594"
mRNA complement(<665218..>667137)
/gene="STR2"
/locus_tag="YJR130C"
/product="cystathionine gamma-synthase"
/transcript_id="NM_001181788.1"
/db_xref="GeneID:853594"
CDS complement(665218..667137)
/gene="STR2"
/locus_tag="YJR130C"
/EC_number="2.5.1.48"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0003962
cystathionine gamma-synthase activity [PMID:10821189]"
/experiment="EXISTENCE:mutant phenotype:GO:0006790 sulfur
compound metabolic process [PMID:10821189]"
/experiment="EXISTENCE:mutant phenotype:GO:0019346
transsulfuration [PMID:10821189]"
/note="Cystathionine gamma-synthase, converts cysteine
into cystathionine; STR2 has a paralog, YML082W, that
arose from the whole genome duplication"
/codon_start=1
/product="cystathionine gamma-synthase"
/protein_id="NP_012664.1"
/db_xref="GeneID:853594"
/db_xref="SGD:S000003891"
/translation="MISRTIGESIPPNTKHAVSVCLPTWEATVGYEEGESSIINSLTT
GYPRFFIHKSIKKLCEILSAKYSMEDEACLCFPSYKVANRCREFIKVKTGLSTKVRIL
QLCTPKPMNQEEKLWRRECKITVVFVDQEIFPVMKQYWQHSGEIVSSRMAEYILHELQ
VKDNLKKMETVDNGKKFMTEDENRVNEEYIETRFGRNLNFLAADKAKYLIRKRIATKV
VEKIDSEGLSDLFSFEHYNESNGPFNVGSGEALDDDQLNSDIPAETITSMGESGSNST
FENTATDDLKFHVNPNTDVYLFPSGMASIFTAHRLLLNFDAKRLSRSSSRQDKLIGYG
PPFKKTVMFGFPYTDTLSILRKFNHTHFLGQGDSTSMNALKNILHSGEQILAVFIEAP
SNPLLKMGDLQELKRLSDLYSFYIVVDETVGGFVNIDVLPYADIVCSSLTKIFSGDSN
VIAGSLVLNPRGKIYEFARKFMKTEDGYEDCLWCEDALCLERNSRDFVERTIKVNTNT
DILLKRVLLPQVGKLFKKIYYPSLTSEDTKRNYDSVMSTKDGGYGGLFSLTFFNIEEA
KKFFNNLELCKGPSLGTNFTLACPYAIIAHYQELDEVAQYGVETNLVRVSVGLENSDV
LCNVFQRAIEKALGE"
gene <667644..>669293
/gene="MNS1"
/locus_tag="YJR131W"
/db_xref="GeneID:853595"
mRNA <667644..>669293
/gene="MNS1"
/locus_tag="YJR131W"
/product="mannosyl-oligosaccharide 1,2-alpha-mannosidase"
/transcript_id="NM_001181789.3"
/db_xref="GeneID:853595"
CDS 667644..669293
/gene="MNS1"
/locus_tag="YJR131W"
/EC_number="3.2.1.113"
/experiment="EXISTENCE:direct assay:GO:0004571
mannosyl-oligosaccharide 1,2-alpha-mannosidase activity
[PMID:8910350]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:8864657]"
/experiment="EXISTENCE:mutant phenotype:GO:0004571
mannosyl-oligosaccharide 1,2-alpha-mannosidase activity
[PMID:1714453|PMID:8439291]"
/experiment="EXISTENCE:mutant phenotype:GO:0035977 protein
deglycosylation involved in glycoprotein catabolic process
[PMID:9732283]"
/experiment="EXISTENCE:mutant phenotype:GO:0036503 ERAD
pathway [PMID:8905927]"
/note="Alpha-1,2-mannosidase; involved in ER-associated
protein degradation (ERAD); catalyzes the removal of one
mannose residue from a glycosylated protein, converting
the modification from Man9GlcNAc to Man8GlcNAc; catalyzes
the last step in glycoprotein maturation in the ER and is
critical for ER protein degradation"
/codon_start=1
/product="mannosyl-oligosaccharide 1,2-alpha-mannosidase"
/protein_id="NP_012665.3"
/db_xref="GeneID:853595"
/db_xref="SGD:S000003892"
/translation="MKNSVGISIATIVAIIAAIYYVPWYEHFERKSPGAGEMRDRIES
MFLESWRDYSKHGWGYDVYGPIEHTSHNMPRGNQPLGWIIVDSVDTLMLMYNSSTLYK
SEFEAEIQRSEHWINDVLDFDIDAEVNVFETTIRMLGGLLSAYHLSDVLEVGNKTVYL
NKAIDLGDRLALAFLSTQTGIPYSSINLHSGQAVKNHADGGASSTAEFTTLQMEFKYL
AYLTGNRTYWELVERVYEPLYKNNDLLNTYDGLVPIYTFPDTGKFGASTIRFGSRGDS
FYEYLLKQYLLTHETLYYDLYRKSMEGMKKHLLAQSKPSSLWYIGEREQGLHGQLSPK
MDHLVCFMGGLLASGSTEGLSIHEARRRPFFSLSLERKSDWDLAKGITDTCYQMYKQS
SSGLAPEIVVFNDGNIKQDGWWRSSVGDFFVKPLDRHNLQRPETVESIMFMYHLSHDH
KYREWGAEIATSFFENTCVDCNDPKLRRFTSLSDCITLPTKKSNNMESFWLAETLKYL
YILFLDEFDLTKVVFNTEAHPFPVLDEEILKSQSLTTGWSL"
gene <669522..>672668
/gene="NMD5"
/locus_tag="YJR132W"
/gene_synonym="KAP119"
/db_xref="GeneID:853596"
mRNA <669522..>672668
/gene="NMD5"
/locus_tag="YJR132W"
/gene_synonym="KAP119"
/product="Nmd5p"
/transcript_id="NM_001181790.2"
/db_xref="GeneID:853596"
CDS 669522..672668
/gene="NMD5"
/locus_tag="YJR132W"
/gene_synonym="KAP119"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9852143]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922|PMID:9852143]"
/experiment="EXISTENCE:direct assay:GO:0034399 nuclear
periphery [PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0061608 nuclear
import signal receptor activity [PMID:9852143]"
/experiment="EXISTENCE:mutant phenotype:GO:0006606 protein
import into nucleus [PMID:9755161]"
/experiment="EXISTENCE:mutant phenotype:GO:0061608 nuclear
import signal receptor activity [PMID:9755161]"
/note="Karyopherin; a carrier protein involved in nuclear
import of proteins; importin beta homolog"
/codon_start=1
/product="Nmd5p"
/protein_id="NP_012666.2"
/db_xref="GeneID:853596"
/db_xref="SGD:S000003893"
/translation="MDITELLQCFACTLDHNAAVRTNAETHLKNASKVPGFLGACLDI
IAADEVPENIKLSASLYFKNKITYGWSAGARQGSNELLDSHVDPDEKPVVKDMLIKTM
VSVSKTSPRCIRVLKSALTVIISEDYPSKKWGNLLPNSLELLANEDITVTYVGLLCLA
EIFRTYRWKNNDERQDLEELILNYFPALLNYGANVLFQDGKYMNNEQIGELVKLIIKI
YKFVSYHDLPFTLQRSESFTPWACFFVSIIQQPLPQEVLAISDIEVRSKNPWVKCKKW
ALANLYRLFQRYASTSLTRKFQYDEFKQMYCEEFLTQFLQVVFDQIEKWGTGQLWLSD
ECLYYILNFVEQCVVQKTTWKLVGPHYNVILQHVIFPLLKPTAETLEAFDNDPQEYIN
RNMDFWDVGYSPDLAALALLTTCVTKRGKTTLQPTLEFMVSTLQSAVGDYNNIMLDNA
LQIESCLRIFSSIIDRLITKDSPFASEMEKFILTYVLPFFKSQYGFLQSRVCDICSKL
GSMDFKDPVITSTIYEGVMNCLNNSSNSLPVELTAALALQTFISDDQFNMKLSEHVVP
TMQKLLSLSNDFESDVISGVMQDFVEQFAEQLQPFGVELMNTLVQQFLKLAIDLHETS
NLDPDSFTNVDSIPDESDKQMAALGILSTTISILLSFENSPEILKNLEQSFYPAAEFI
LKNDIEDFYRECCEFVENSTFLLRDITPISWKILELIGECNRKPDSMVSYYLSDFMLA
LNNILIYGRNELKKNEFYTKIIFEIYQKAVTAEDNSLDDLRVVFDLSQELVLALDDSL
PQQYRERLLADVVGSILTQKNELKTNVVFSVTAFNVVISNMITEPLITLQYLKQQGCL
EIFFQTWITDYIPNYKRCYDIKLSVLALLKIILKLESNDYSVLNLENLVPQLGSIVTQ
LASRLPTALRQLANQRKEFSSSGFEEDTKWDENFLDVGDDDENDDEGDLTEKYLELIK
NRADSLDFVDGYDAKETFDDLEEDPLTGSILDTVDVYKVFKESIANLQHVDSNRYQGI
LRHLTPADQELFMGIMNA"
gene <672991..>673620
/gene="XPT1"
/locus_tag="YJR133W"
/db_xref="GeneID:853597"
mRNA <672991..>673620
/gene="XPT1"
/locus_tag="YJR133W"
/product="xanthine phosphoribosyltransferase"
/transcript_id="NM_001181791.1"
/db_xref="GeneID:853597"
CDS 672991..673620
/gene="XPT1"
/locus_tag="YJR133W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0004422
hypoxanthine phosphoribosyltransferase activity
[PMID:10217799]"
/experiment="EXISTENCE:genetic interaction:GO:0046100
hypoxanthine metabolic process [PMID:10217799]"
/experiment="EXISTENCE:mutant phenotype:GO:0000310
xanthine phosphoribosyltransferase activity
[PMID:10217799]"
/experiment="EXISTENCE:mutant phenotype:GO:0032265 XMP
salvage [PMID:10217799]"
/note="Xanthine-guanine phosphoribosyl transferase;
required for xanthine utilization and for optimal
utilization of guanine"
/codon_start=1
/product="xanthine phosphoribosyltransferase"
/protein_id="NP_012667.1"
/db_xref="GeneID:853597"
/db_xref="SGD:S000003894"
/translation="MAENERMYISYNNIHKLCQGVAKHILARNERPDIIIAITGGGMI
PARIIRSFLKTKGQKNIPIQAIGLSLYEDLGLDNSVETIGKEVIRTQWLDFGALNQHF
DSLIGKKVLIVDEVDDTRTTLHYAVSELEKEIAEQQKVLNRMSEETVISIFVLHNKDK
PKRAGLPDSMMNSGRYIAAQTVPDKWLCYPWDAEDIEEHTMLAKAQGHD"
gene complement(<673729..>675852)
/gene="SGM1"
/locus_tag="YJR134C"
/db_xref="GeneID:853598"
mRNA complement(<673729..>675852)
/gene="SGM1"
/locus_tag="YJR134C"
/product="Sgm1p"
/transcript_id="NM_001181792.3"
/db_xref="GeneID:853598"
CDS complement(673729..675852)
/gene="SGM1"
/locus_tag="YJR134C"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:14562095]"
/note="hypothetical protein; required for wild-type growth
rate on galactose and mannose; localizes to COPI coated
vesicles and the Golgi apparatus"
/codon_start=1
/product="Sgm1p"
/protein_id="NP_012668.3"
/db_xref="GeneID:853598"
/db_xref="SGD:S000003895"
/translation="MSKKLSLEERLSLATKKGRKKNKRSTSNLSSPSPVVLSNNEQES
ARTSIDDAAAGVVSIDNAENIDDPAVRSESTVEGDTGKADSIAVDDVVHPDHNRTDCF
DDTMVSLPTWLPKNYTEFTVEELVKEISPEYLRLNKQIDDLTNELNRKSQIETTDSSF
FKLIKEKDDLIDQLRKEGAKLAETELRQSNQIKALRTKVKDLEYEVSELNDSSAQSVE
NYNELQSLYHNIQGQLAEATNKLKDADKQKESLETLEKNIKEKDDLITILQQSLDNMR
TLLEKEKSEFQTEKKALQEATVDQVTTLETKLEQLRIELDSSTQNLDAKSNRDFVDDQ
QSYEEKQHASFQYNRLKEQLESSKANWDSIEYALNTKIVNLENRFESTMKEKNDIEEK
YQTALRSSETLGKQLEKEKENHSKAVLEVKDLERRAETLKSSLQSISDDYNLLKKKYE
IQRSQLEQKENELKPHQENSNEKIIDKIPVELTDSLNSMEGNIEDEWTLPQENSMLSL
SMSKLGELESDPSLKPIYNESHETICSEESQHFDRKNVDFSIDDIPEEAAALQAIREG
ESMNSLNNTSIPYRRASVQLSNSNGHISAHLVNKLSTELKRLEGELSASKELYDNLLK
EKTKANDEILRLLEENDKFNEVNKQKDDLLKRVEQMQSKLETSLQLLGEKTEQVEELE
NDVSDLKEMMHQQVQQMVEMQGKMR"
gene complement(<676059..>676778)
/gene="MCM22"
/locus_tag="YJR135C"
/db_xref="GeneID:853599"
mRNA complement(<676059..>676778)
/gene="MCM22"
/locus_tag="YJR135C"
/product="Mcm22p"
/transcript_id="NM_001181793.1"
/db_xref="GeneID:853599"
CDS complement(676059..676778)
/gene="MCM22"
/locus_tag="YJR135C"
/experiment="EXISTENCE:direct assay:GO:0000776 kinetochore
[PMID:11782448]"
/experiment="EXISTENCE:mutant phenotype:GO:0007059
chromosome segregation [PMID:11782448]"
/experiment="EXISTENCE:mutant phenotype:GO:0034087
establishment of mitotic sister chromatid cohesion
[PMID:19730685]"
/note="Outer kinetochore protein and component of the Ctf3
subcomplex; binds to centromeric DNA in a Ctf19p-dependent
manner; involved in chromosome segregation and
minichromosome maintenance; orthologous to human
centromere constitutive-associated network (CCAN) subunit
CENP-K and fission yeast sim4"
/codon_start=1
/product="Mcm22p"
/protein_id="NP_012669.1"
/db_xref="GeneID:853599"
/db_xref="SGD:S000003896"
/translation="MDVEKDVLDVYIKNLENQIGNKRYFLKQAQGAIDEITKRSLDTE
GKPVNSEVFTELLRKPMFFSERADPIGFSLTSNFLSLRAQSSSEWLSLMNDQSVDQKA
MLLLQNNINSDLKELLRKLQHQMTIMDSKKQDHAHIRTRKARNKELWDSLADFLKGYL
VPNLDDNDESIDSLTNEVMLLMKRLIEHDLNLTLNDFSSKTIPIYRLLLRANIITVIE
GSTNPGTKYIKLIDFNETSLT"
gene <676971..>677234
/gene="TIM8"
/locus_tag="YJR135W-A"
/db_xref="GeneID:853600"
mRNA <676971..>677234
/gene="TIM8"
/locus_tag="YJR135W-A"
/product="protein transporter TIM8"
/transcript_id="NM_001184382.1"
/db_xref="GeneID:853600"
CDS 676971..677234
/gene="TIM8"
/locus_tag="YJR135W-A"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0042719
mitochondrial intermembrane space chaperone complex
[PMID:12221072]"
/experiment="EXISTENCE:direct assay:GO:0045039 protein
insertion into mitochondrial inner membrane
[PMID:10469659|PMID:10051608]"
/experiment="EXISTENCE:direct assay:GO:0140318 protein
transporter activity [PMID:10051608|PMID:10469659]"
/experiment="EXISTENCE:mutant phenotype:GO:0045039 protein
insertion into mitochondrial inner membrane
[PMID:10469659]"
/experiment="EXISTENCE:mutant phenotype:GO:0140318 protein
transporter activity [PMID:10469659]"
/experiment="EXISTENCE:physical interaction:GO:0042719
mitochondrial intermembrane space chaperone complex
[PMID:10051608]"
/note="Mitochondrial intermembrane space protein; forms a
complex with Tim13p that delivers a subset of hydrophobic
proteins to the TIM22 complex for inner membrane
insertion; homolog of human TIMM8A, implicated in
Mohr-Tranebjaerg syndrome, also known as
deafness-dystonia-optic neuronopathy (DDON) syndrome;
human TIMM8A can complement yeast null mutant"
/codon_start=1
/product="protein transporter TIM8"
/protein_id="NP_058168.1"
/db_xref="GeneID:853600"
/db_xref="SGD:S000007348"
/translation="MSSLSTSDLASLDDTSKKEIATFLEGENSKQKVQMSIHQFTNIC
FKKCVESVNDSNLSSQEEQCLSNCVNRFLDTNIRIVNGLQNTR"
gene complement(<677441..>678706)
/gene="TTI2"
/locus_tag="YJR136C"
/db_xref="GeneID:853601"
mRNA complement(<677441..>678706)
/gene="TTI2"
/locus_tag="YJR136C"
/product="Tti2p"
/transcript_id="NM_001181794.3"
/db_xref="GeneID:853601"
CDS complement(677441..678706)
/gene="TTI2"
/locus_tag="YJR136C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0110078 TTT Hsp90
cochaperone complex [PMID:19040720]"
/experiment="EXISTENCE:mutant phenotype:GO:0006974 DNA
damage response [PMID:27172216]"
/experiment="EXISTENCE:mutant phenotype:GO:0031669
cellular response to nutrient levels [PMID:27172216]"
/experiment="EXISTENCE:mutant phenotype:GO:0034605
cellular response to heat [PMID:27172216]"
/experiment="EXISTENCE:mutant phenotype:GO:0071472
cellular response to salt stress [PMID:27172216]"
/note="Subunit of the TTT complex and regulator of
telomere length; subunit of the TTT Hsp90 cochaperone
complex that is involved in chromatin remodeling and
telomeric chromatin regulation; telomere length regulator
involved in the stability or biogenesis of PIKKs such as
TORC1; involved in the cellular stress response; may
interact with Rsm23p; GFP-fusion protein localizes to the
cytoplasm; similar to S. pombe Tti2p"
/codon_start=1
/product="Tti2p"
/protein_id="NP_012670.3"
/db_xref="GeneID:853601"
/db_xref="SGD:S000003897"
/translation="MTAVTDIIDELNDSSLSSTRLRELCLQLRKKTDTGCAITVSDEV
NLIESLSYHSISPGVDIQINTDVLQTIDYYFQRNKSEHDEIMCVLISKLQPLLLKRKS
NFELKEQRNLGLKPTLGMSLKEDNLMQAWVSQGGLKGIPLFYVILLHLKRRDISTNLS
WIIPGILNILDDTTDIRRIKLRGVLLLQTLLNHTFMNETNDSKWIQFSSTGLFPLFEK
TLINMCYFLPPSYNADETIAIWRVVFPTIQSLYKVEFLDNYTKYQYHLEKFMSEIILQ
NIIPRASLAYENLTLYALECTMNILRLQREGSVVHLQRLIFVLGEYIVRNPFYTTFPK
LISKTLSVVSTLIKVCPNERIVAHRFDILSLILVTYDKCSQEDALNESILQQCKETIS
WLLNCDCAMGEQLSTLSKQPRFQLLFEFS"
gene complement(<678957..>683285)
/gene="MET5"
/locus_tag="YJR137C"
/gene_synonym="ECM17"
/db_xref="GeneID:853602"
mRNA complement(<678957..>683285)
/gene="MET5"
/locus_tag="YJR137C"
/gene_synonym="ECM17"
/product="sulfite reductase (NADPH) subunit beta"
/transcript_id="NM_001181795.1"
/db_xref="GeneID:853602"
CDS complement(678957..683285)
/gene="MET5"
/locus_tag="YJR137C"
/gene_synonym="ECM17"
/EC_number="1.8.1.2"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0004783 sulfite
reductase (NADPH) activity [PMID:6751400]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0009337 sulfite
reductase complex (NADPH) [PMID:6751400|PMID:9003798]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0000097 sulfur
amino acid biosynthetic process [PMID:1479340]"
/experiment="EXISTENCE:mutant phenotype:GO:0000103 sulfate
assimilation [PMID:19236486]"
/note="Sulfite reductase beta subunit; involved in amino
acid biosynthesis, transcription repressed by methionine"
/codon_start=1
/product="sulfite reductase (NADPH) subunit beta"
/protein_id="NP_116579.1"
/db_xref="GeneID:853602"
/db_xref="SGD:S000003898"
/translation="MTASDLLTLPQLLAQYSSSAPQNKVFYTTSTKNSHSSFKGLESV
ATDATHLLNNQDPLNTIKDQLSKDILTTVFTDETTLVKSIHHLYSLPNKLPLVITVDL
NLQDYSAIPALKDLSFPILISSDLQTAISNADSSYKIATSSLTPVFHFLNLEKIGTST
AIEQDIDFPTLEIANEETKVALSEATDSLTNFELVKGKESITTVIVNLSPYDAEFSSV
LPSNVGLIKIRVYRPWNFSKFLEILPSSVTKIAVLQGVSKKSQSNEFQPFLLDFFGNF
NELVSRNIEQVVLTNIGNVNDYGNVINTVISNINKKEPDNNLFLGESNEKAEEQAEVT
QLISSVKKVVNLEDAYIKVLKQLFSSNLQILNQFSSETIEPSNPEFGFGRFLKQEAQR
EELISLAKTSLDPSLYLSEDANKIVQLLSKWLSFNGRDLDEAQLQEANATGLEIFQLL
QSNQDSSTVLKFLKIAPTSDSFIFKSSWLIGSDAWSYDLGHSGIQQVLSSRKNINVLL
IDSEPYDHRKQNQDRKKDVGLYAMNYYSAYVASVAVYASYTQLLTAIIEASKYNGPSI
VLAYLPYNSENDTPLEVLKETKNAVESGYWPLYRFNPVYDDPSTDKEAFSLDSSVIRK
QLQDFLDRENKLTLLTRKDPSLSRNLKQSAGDALTRKQEKRSKAAFDQLLEGLSGPPL
HVYYASDGGNAANLAKRLAARASARGLKATVLSMDDIILEELPGEENVVFITSTAGQG
EFPQDGKSFWEALKNDTDLDLASLNVAVFGLGDSEYWPRKEDKHYFNKPSQDLFKRLE
LLSAKALIPLGLGDDQDADGFQTAYSEWEPKLWEALGVSGAAVDDEPKPVTNEDIKRE
SNFLRGTISENLKDTSSGGVTHANEQLMKFHGIYTQDDRDIREIRKSQGLEPYYMFMA
RARLPGGKTTPQQWLALDHLSDTSGNGTLKLTTRATFQIHGVLKKNLKHTLRGMNAVL
MDTLAAAGDVNRNVMVSALPTNAKVHQQIADMGKLISDHFLPKTTAYHEVWLEGPEEQ
DDDPSWPSIFENRKDGPRKKKTLVSGNALVDIEPIYGPTYLPRKFKFNIAVPPYNDVD
VLSIDVGLVAIVNPETQIVEGYNVFVGGGMGTTHNNKKTYPRLGSCLGFVKTEDIIPP
LEGIVIVQRDHGDRKDRKHARLKYTVDDMGVEGFKQKVEEYWGKKFEPERPFEFKSNI
DYFGWIKDETGLNHFTAFIENGRVEDTPDLPQKTGIRKVAEYMLKTNSGHFRLTGNQH
LVISNITDEHVAGIKSILKTYKLDNTDFSGLRLSSSSCVGLPTCGLAFAESERFLPDI
ITQLEDCLEEYGLRHDSIIMRMTGCPNGCSRPWLGELALVGKAPHTYNLMLGGGYLGQ
RLNKLYKANVKDEEIVDYIKPLFKRYALEREEGEHFGDFCIRVGIIKPTTEGKYFHED
VSEDAY"
rep_origin 684009..684098
/note="ARS1021; Autonomously Replicating Sequence"
/db_xref="SGD:S000007553"
gene <684567..>689321
/gene="IML1"
/locus_tag="YJR138W"
/gene_synonym="SEA1"
/db_xref="GeneID:853603"
mRNA <684567..>689321
/gene="IML1"
/locus_tag="YJR138W"
/gene_synonym="SEA1"
/product="GTPase-activating protein IML1"
/transcript_id="NM_001181796.2"
/db_xref="GeneID:853603"
CDS 684567..689321
/gene="IML1"
/locus_tag="YJR138W"
/gene_synonym="SEA1"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005096 GTPase
activator activity [PMID:23716719]"
/experiment="EXISTENCE:direct assay:GO:0035859
Seh1-associated complex [PMID:21454883]"
/experiment="EXISTENCE:direct assay:GO:1990130 GATOR1
complex [PMID:21900499]"
/experiment="EXISTENCE:genetic interaction:GO:0032007
negative regulation of TOR signaling [PMID:23716719]"
/experiment="EXISTENCE:genetic interaction:GO:0051058
negative regulation of small GTPase mediated signal
transduction [PMID:23716719]"
/experiment="EXISTENCE:mutant phenotype:GO:0005096 GTPase
activator activity [PMID:23716719]"
/experiment="EXISTENCE:mutant phenotype:GO:0006995
cellular response to nitrogen starvation [PMID:25046117]"
/experiment="EXISTENCE:mutant phenotype:GO:0010508
positive regulation of autophagy [PMID:25046117]"
/experiment="EXISTENCE:mutant phenotype:GO:0032007
negative regulation of TOR signaling [PMID:23974112]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress [PMID:35158208]"
/experiment="EXISTENCE:mutant phenotype:GO:1904262
negative regulation of TORC1 signaling [PMID:23716719]"
/experiment="EXISTENCE:mutant phenotype:GO:2000785
regulation of autophagosome assembly [PMID:21900499]"
/note="GTPase-activating protein (GAP) subunit of the
Iml1p/SEACIT complex; SEACIT (Iml1p-Npr2p-Npr3p) is
required for non-nitrogen-starvation (NNS)-induced
autophagy, and is a subcomplex of the SEA, a
coatomer-related complex that associates with the vacuole;
Iml1p functions as a GAP for the Rag family GTPase Gtr1p
(EGOC subunit) in SEACIT, resulting in the inhibition of
TORC1 signaling in response to amino acid deprivation;
role in the oxidative stress response"
/codon_start=1
/product="GTPase-activating protein IML1"
/protein_id="NP_012672.1"
/db_xref="GeneID:853603"
/db_xref="SGD:S000003899"
/translation="MFAKLHGKKQRPISSINSQTPRTSNTTHANSISLSSGNLIVGSN
RNLRQKKEQFGSQQRASGRKLISNKENDDNVNNGGDNNYDNGERVHRHHIPGLKIKAY
QAELGYHESRFSENLVMLNLVEFPDIKPGDLVELKTYHKNPSASNGDKKIYFIAKDFD
GETKRRAKTSNVSILSGQLQTLLDLPSRSRIWIKLKPNKFDLQADVVEFNIKDCLLNR
GDMWVLSSKLVDTCVFMDQRLAFLDSIRGTIKGIYRNGKKIVSGYIGEQTRIIFRSES
ARLIFLIQITDEMWNFEETGEQLFQKMVNSFFPKIFKKWKDVDTHHTITIAFAISMDL
SDTSFKDLTPGESLKNSQDYFRIVVDQVSIIHWVDIMETLREEFMEIRKDLLNKQTDK
GYSVANGRFSPVIKSNFLELVNFATTILTDPFKQLDLRHTTTHVMIISPGSGLFDVDY
SLLRLTGKKLLSLEMTMDLICLSKAPLHIVPLFRYRDFENKLHHCVPLWLSVFFWNDH
DKKSNSEWTPRCKIYDLQMMGITENELIREVDVEYLQLNKKVKSLSEFMNDYDKNAFE
VKILCAGSNTKQSKKLNSKFDTVFENDVVVKARKIPATATTTHGNTKFIWRGPKVALP
AIKDIQKPNVIPDLSIKTIEASFYDDCNTTNDKISTPTTSNNDNLEMNDSLVSVRSAD
NQNTSLALDSLKGLSKRNSLKDFTQRVITKFISNIDTSKNKKIKSTLLRDDVDNSPLG
SNTPLPSSESKISGLKLQQKGLADENVISKRGNLIIKKNLSIFGLPSNEIMSGSPSSY
LGSSHTRTSSKLSNMSDKAAFITEGQKSKHDDSNTYSLTQQLKHRISETWVDIKSPSI
PVSSEFANELLPIRWKDVWPKYVARKYSKWRSFTTPAELPITISDFPSKDDFDRNFIF
RNHSVTLNTDQEQYNQTYKDLLRDMIYMRLLTGFQICVGRQVEKIELSRESGESETVV
NKYLDFNQNDAFKLYLMIDSEIHRITCSSSGIIDVERYLRKDEANLFDQVPSYIPLVK
TRYESSFRDAMIDPLHVKRESLNWNQIDQVLAGYGDNLIDRKWHGFRAKYVVLPTDIP
PNTYSMVINGKSETLNPEEIRVEGLRRLIGSITRSRLRTEKEKKGRKTKREEIQPEVM
FYTGPLYNFINEQQTSLESSAINFKDSIFVNDNNLLNRNVELSKLAYQIQRGEDRITL
VNRKWHWKKHEKCFVGSEMVNWLIRNFSDIDTREDAIKYGQKVMKEGLFVHVLNKHNF
LDGHYFYQFSPEYVMDTNKLEKTNSHRSTLSDPKQMLRKASTGSSNDPSAMTPFSSVV
PAISASNASVADAKEPSRPILMLSNSLVIDVDPAGKSSKQESCTVHYDRVHNPDHCFH
IRLEWLTTTPKLIDDLVGNWSRLCERYGLKMIEIPWEELCTIPSVNPFHSFVEIKLAI
NPWEDPEFKDRELFAKSKFYYHVYLLKASGFLLDNRASKFLQNQDIEFDIMYSWGKPQ
FKYVQYIHHTGAYVAELRENGCLFLAPNNIYISRVNPGNIIGKIHSASSSSLDAQKVI
LNFKSTCLDYQKLRSIFLDAKEMWITGKIVED"
gene complement(<689445..>690524)
/gene="HOM6"
/locus_tag="YJR139C"
/gene_synonym="THR6"
/db_xref="GeneID:853604"
mRNA complement(<689445..>690524)
/gene="HOM6"
/locus_tag="YJR139C"
/gene_synonym="THR6"
/product="homoserine dehydrogenase"
/transcript_id="NM_001181797.3"
/db_xref="GeneID:853604"
CDS complement(689445..690524)
/gene="HOM6"
/locus_tag="YJR139C"
/gene_synonym="THR6"
/EC_number="1.1.1.3"
/experiment="EXISTENCE:direct assay:GO:0004412 homoserine
dehydrogenase activity [PMID:11341915]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0009086
methionine biosynthetic process [PMID:4380684]"
/experiment="EXISTENCE:mutant phenotype:GO:0009088
L-threonine biosynthetic process [PMID:4380684]"
/experiment="EXISTENCE:mutant phenotype:GO:0009090
L-homoserine biosynthetic process [PMID:4380684]"
/note="Homoserine dehydrogenase (L-homoserine:NADP
oxidoreductase); dimeric enzyme that catalyzes the third
step in the common pathway for methionine and threonine
biosynthesis; enzyme has nucleotide-binding, dimerization
and catalytic regions"
/codon_start=1
/product="homoserine dehydrogenase"
/protein_id="NP_012673.3"
/db_xref="GeneID:853604"
/db_xref="SGD:S000003900"
/translation="MSTKVVNVAVIGAGVVGSAFLDQLLAMKSTITYNLVLLAEAERS
LISKDFSPLNVGSDWKAALAASTTKTLPLDDLIAHLKTSPKPVILVDNTSSAYIAGFY
TKFVENGISIATPNKKAFSSDLATWKALFSNKPTNGFVYHEATVGAGLPIISFLREII
QTGDEVEKIEGIFSGTLSYIFNEFSTSQANDVKFSDVVKVAKKLGYTEPDPRDDLNGL
DVARKVTIVGRISGVEVESPTSFPVQSLIPKPLESVKSADEFLEKLSDYDKDLTQLKK
EAATENKVLRFIGKVDVATKSVSVGIEKYDYSHPFASLKGSDNVISIKTKRYTNPVVI
QGAGAGAAVTAAGVLGDVIKIAQRL"
gene complement(<690750..>695696)
/gene="HIR3"
/locus_tag="YJR140C"
/gene_synonym="HPC1"
/db_xref="GeneID:853605"
mRNA complement(<690750..>695696)
/gene="HIR3"
/locus_tag="YJR140C"
/gene_synonym="HPC1"
/product="Hir3p"
/transcript_id="NM_001181798.1"
/db_xref="GeneID:853605"
CDS complement(690750..695696)
/gene="HIR3"
/locus_tag="YJR140C"
/gene_synonym="HPC1"
/experiment="EXISTENCE:direct assay:GO:0000417 HIR complex
[PMID:16303565|PMID:16264190]"
/experiment="EXISTENCE:direct assay:GO:0003677 DNA binding
[PMID:16264190]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9001207]"
/experiment="EXISTENCE:direct assay:GO:0006325 chromatin
organization [PMID:16303565|PMID:16264190]"
/experiment="EXISTENCE:genetic interaction:GO:0006368
transcription elongation by RNA polymerase II
[PMID:16449659]"
/experiment="EXISTENCE:mutant phenotype:GO:0000082 G1/S
transition of mitotic cell cycle [PMID:1406694]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:1406694]"
/experiment="EXISTENCE:mutant phenotype:GO:0003714
transcription corepressor activity [PMID:9001207]"
/experiment="EXISTENCE:mutant phenotype:GO:0031491
nucleosome binding [PMID:16264190]"
/experiment="EXISTENCE:physical interaction:GO:0000417 HIR
complex [PMID:16264190]"
/note="Subunit of the HIR complex; a nucleosome assembly
complex involved in regulation of histone gene
transcription; involved in position-dependent gene
silencing and nucleosome reassembly; ortholog of human
CABIN1 protein"
/codon_start=1
/product="Hir3p"
/protein_id="NP_012674.1"
/db_xref="GeneID:853605"
/db_xref="SGD:S000003901"
/translation="MSMFNALNSNIEGEQYEAEEHSRELQIEQSFNILQDALIDLKNK
DFEKSDSKFQELFQIDVVKPDRWGMYRNSSPTLDNLRYLCYRNRGMYYHLYLENNYER
LNSQELVNCILKAVENLVESIQHSDADFAVTDLLARIFKSFNSVKLERLISEYEFTKQ
ENLSLLLGRHRKFLLNDLTLMMNNYVELTNKLLVPNLSDNTIFERYHLEKYKDIKPEP
LAFGPILSRISEMKKQDEEIMKKLDVFNVTLNEESWDEVAKALKNLLPSVKTSSLIGR
NMDPYNEIEEPIEAVKFELSEAINNTPSLDRESERQEEEQDNESVRADDKSGNLAPSD
IQTNEEARPNKRTDEHIDSTKPLQRSSKRFKEREQENSKELVMDVHKRFFGEFNTLLS
YIHILPFCDFDTFASKFIIGSSDKQPEKFIPYTDLYECLKSWSSRYTDIFNQNDYLSS
GSNENEELFQLNALLKSNAFDDKESFPRYLNDLDSDHIRSFISEVNAGNLHFHQVRLK
LLFKLLGTYDEGNGRRLIIDYLWESQLLKIVLWFVFGIESNIFALINKNKRQCKYLAL
SIYELLVNHLGNIVEEITNKRIQGHKSADLKSQRNKVEKRIRSWHTLLEQIADEKDKE
LYVHFQWTHYCFLQYTCDIVDSRLSETLTSLENTIKDSDSSLDIAYPNYRHIPALNLN
TVQSQKRKIRIIQNITVEDISEDTNSDTHSENHLETLEKVLLHILHPSTNHSNIDEEM
VSFIFNSPFLLKIRLWGVLFSSYVKKSSIQDVQRIYFHVLDFMKGALTSPVYKESNPH
GRHQMLLTVLTAIGYLSSQLTAILNSNRWESSDFVLEDYMFEKLLQTFFFFYTVLFYE
SSAVNDVSNKSFFKRASKSSGKMKDIMIDLATLILYYYDLQAKLRTPAEQGIETTELI
WSLHTLFGHFHFCDASNGKFLDLAEKLLCQFINNDSFLQLKQILWCRYHYAIASDNFS
PDLHDTKAVEMEKIHSLPLGTYLIKLQYQNKNPYLSSSKTTLKQIMDNIIEKIGDPST
LDNHIISRNSFLLNEYLSRPITADLLKHTFSGATSLYLTSPNDELQQGMTAGLFYVSS
LQSLGLYKMRKKSMQARPSELDSIIRMLKNDIIYNTNRFESWILLGKCYSYIVEDDLI
WTSDKITVPEKKDVIALTQRKAILCYLMAISIYYSKLDRTIDDKKIILEALDDLGSML
ISGYYNPMNKLCFSWKSSAENTMRLSETGEVVMEKTKKITTISDFNIEQSIFLCFNRA
CSLSGDIKSQDDVFVLNWSSFYNLAKFFFKTDGGNNCKLVAKYITQGCQIAYESSPAK
DPIIEPHYLLVNACYKWVKRGVIGVNEALTLLSKDNQFFQEQEEFWVNDEGLAWDYQE
KFFFDKIIRLLRHLLSVDKKKWQHRPRYRIARILFDDLGDVNGALEEMDSLISAKSIN
KNLVNIWKPDFERPGKHFIYTYQYLVLYLDLLFAIKDFNTTGLVIKKLRRFGSGTVNV
NELLERAINVYTQSAKIKLQLQDKSYVEQILPTLNYQEFLKISEQLNQVFDQGKYPEE
ISSGLKLAFQLKKGHSGIAFDSVCLGIYFEYLYFPLARQDQSLTDVNDENNPALPSSG
SVTSKSTPDPTSKPSAIKKRVTKKEVFDRVRLLVDKIT"
gene <695906..>696949
/gene="IPA1"
/locus_tag="YJR141W"
/db_xref="GeneID:853606"
mRNA <695906..>696949
/gene="IPA1"
/locus_tag="YJR141W"
/product="putative polyadenylation protein"
/transcript_id="NM_001181799.1"
/db_xref="GeneID:853606"
CDS 695906..696949
/gene="IPA1"
/locus_tag="YJR141W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:29519818]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:29519818]"
/experiment="EXISTENCE:mutant phenotype:GO:0031397
negative regulation of protein ubiquitination
[PMID:32009536]"
/experiment="EXISTENCE:mutant phenotype:GO:0031442
positive regulation of mRNA 3'-end processing
[PMID:32009536]"
/experiment="EXISTENCE:mutant phenotype:GO:0043161
proteasome-mediated ubiquitin-dependent protein catabolic
process [PMID:29519818]"
/experiment="EXISTENCE:physical interaction:GO:0031624
ubiquitin conjugating enzyme binding [PMID:29519818]"
/note="Protein implicated in pre-mRNA processing and
proteasomal degradation; interacts physically and
genetically with mRNA cleavage and polyadenylation
factors; mutant is impaired in pre-mRNA cleavage and
polyadenylation; associates with several Ub-conjugating
enzymes and alters Ub-mediated proteasome activity;
localizes to both the nucleus and cytosol; contains a
HECT_2 domain; homologous to human ubiquitin-protein
ligase, UBE3D, a gene implicated in age-related macular
degeneration"
/codon_start=1
/product="putative polyadenylation protein"
/protein_id="NP_012675.1"
/db_xref="GeneID:853606"
/db_xref="SGD:S000003902"
/translation="MVQYVVEWLPRIQSISVVVEGWKQVEIKNLKDTLMSISGDEEQV
EDILLPVEVEEKVDASYKFKNRGKDLEWMTKLRSKSSKIYDSSIMSLPDGRWTKEELR
SDSDFSIECLNCKQKIISKDNCQVLNDMPSEFWFELMDYWHCHKPDVKEDKSSYTRFE
TLKPSKNEILIGSSYFQGTPATFENVATTKENDNVLCIKCSAVLGQVTAGSLYKLHKW
KLQLIRSGNTYKFPPECDITISLINVVKANSCRYVLVKCKTESLLVWIFSVDIGVTLT
GNKSFKRAMKLLYTNSVTTINRCLNRQVVEELDFQETSFNAFYSALQHTNALLPSSMK
KIGEWTISYTSLI"
gene <697141..>698169
/locus_tag="YJR142W"
/db_xref="GeneID:853607"
mRNA <697141..>698169
/locus_tag="YJR142W"
/product="uncharacterized protein"
/transcript_id="NM_001181800.3"
/db_xref="GeneID:853607"
CDS 697141..698169
/locus_tag="YJR142W"
/experiment="EXISTENCE:direct assay:GO:0044715 8-oxo-dGDP
phosphatase activity [PMID:23834287]"
/note="8-oxo-dGTP diphosphatase of the Nudix hydrolase
family; converts diphosphates of damaged forms of thiamin
to monophosphates; GST fusion protein is a Dbf2p-Mob1p
phosphorylation target in a proteome chip analysis;
synthetic lethal with PH085 deletion; plays a role in
restricting Ty1 transposition"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_012676.3"
/db_xref="GeneID:853607"
/db_xref="SGD:S000003903"
/translation="MKVEKSSKGLEVLVRTQEDDLEGFSFLEIMDRVDPLPLDFENYK
NFKEGIYYMCTHDGTKIGFVLKFAINEMETVCSEIFEETFQLDESRHELRFKSEDFDH
RNNLIDQLARKMYLESSLSGVKGWRNEKYAVWVNKKPYVLVERAVAGVLGIITYGIHI
NGYVLDPKSKKVQFWVPRRSKTKQTWPLMLDNIIAGGLGYPYGIYETVLKESMEEANL
EKSVIEDNIKATGSVSYLYFTGDISVTKFNKESDFIVGEVQYVYDLKLSEDIIPKPND
GEVESFNLFSLQETINALRKKEFKPNCALVMVDFLIRHGYITPENEPNYLELVTRMHR
RLPFPTLN"
gene complement(<698326..>700614)
/gene="PMT4"
/locus_tag="YJR143C"
/db_xref="GeneID:853608"
mRNA complement(<698326..>700614)
/gene="PMT4"
/locus_tag="YJR143C"
/product="dolichyl-phosphate-mannose-protein
mannosyltransferase"
/transcript_id="NM_001181801.1"
/db_xref="GeneID:853608"
CDS complement(698326..700614)
/gene="PMT4"
/locus_tag="YJR143C"
/EC_number="2.4.1.109"
/experiment="EXISTENCE:direct assay:GO:0004169
dolichyl-phosphate-mannose-protein mannosyltransferase
activity [PMID:21956107]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:1900101
regulation of endoplasmic reticulum unfolded protein
response [PMID:21231968]"
/experiment="EXISTENCE:mutant phenotype:GO:0004169
dolichyl-phosphate-mannose-protein mannosyltransferase
activity [PMID:21956107|PMID:8585318]"
/experiment="EXISTENCE:mutant phenotype:GO:0006493 protein
O-linked glycosylation [PMID:8585318]"
/experiment="EXISTENCE:mutant phenotype:GO:0035269 protein
O-linked glycosylation via mannose
[PMID:21231968|PMID:18182384]"
/experiment="EXISTENCE:physical interaction:GO:0097586
dolichyl-phosphate-mannose-protein mannosyltransferase
Pmt4p homodimer complex [PMID:12551906]"
/note="Protein O-mannosyltransferase; transfers mannose
residues from dolichyl phosphate-D-mannose to protein S/T
residues; contains a large TMD containing the catalytic
pocket and a lumenal beta-trefoil-folded MIR domain; acts
as a homodimer utilizing a cooperative dual substrate
trans-recognition mechanism, with substrate bound by the
catalytic pocket of one protomer and the MIR domain of the
other; target for new antifungals"
/codon_start=1
/product="dolichyl-phosphate-mannose-protein
mannosyltransferase"
/protein_id="NP_012677.1"
/db_xref="GeneID:853608"
/db_xref="SGD:S000003904"
/translation="MSVPKKRNHGKLPPSTKDVDDPSLKYTKAAPKCEQVAEHWLLQP
LPEPESRYSFWVTIVTLLAFAARFYKIWYPKEVVFDEVHFGKFASYYLERSYFFDVHP
PFAKMMIAFIGWLCGYDGSFKFDEIGYSYETHPAPYIAYRSFNAILGTLTVPIMFNTL
KELNFRAITCAFASLLVAIDTAHVTETRLILLDAILIISIAATMYCYVRFYKCQLRQP
FTWSWYIWLHATGLSLSFVISTKYVGVMTYSAIGFAAVVNLWQLLDIKAGLSLRQFMR
HFSKRLNGLVLIPFVIYLFWFWVHFTVLNTSGPGDAFMSAEFQETLKDSPLSVDSKTV
NYFDIITIKHQDTDAFLHSHLARYPQRYEDGRISSAGQQVTGYTHPDFNNQWEVLPPH
GSDVGKGQAVLLNQHIRLRHVATDTYLLAHDVASPFYPTNEEITTVTLEEGDGELYPE
TLFAFQPLKKSDEGHVLKSKTVSFRLFHVDTSVALWTHNDELLPDWGFQQQEINGNKK
VIDPSNNWVVDEIVNLDEVRKVYIPKVVKPLPFLKKWIETQKSMFEHNNKLSSEHPFA
SEPYSWPGSLSGVSFWTNGDEKKQIYFIGNIIGWWFQVISLAVFVGIIVADLITRHRG
YYALNKMTREKLYGPLMFFFVSWCCHYFPFFLMARQKFLHHYLPAHLIACLFSGALWE
VIFSDCKSLDLEKDEDISGASYERNPKVYVKPYTVFLVCVSCAVAWFFVYFSPLVYGD
VSLSPSEVVSREWFDIELNFSK"
gene <700882..>701691
/gene="MGM101"
/locus_tag="YJR144W"
/gene_synonym="MGM9"
/db_xref="GeneID:853609"
mRNA <700882..>701691
/gene="MGM101"
/locus_tag="YJR144W"
/gene_synonym="MGM9"
/product="Mgm101p"
/transcript_id="NM_001181802.1"
/db_xref="GeneID:853609"
CDS 700882..701691
/gene="MGM101"
/locus_tag="YJR144W"
/gene_synonym="MGM9"
/experiment="EXISTENCE:direct assay:GO:0003697
single-stranded DNA binding [PMID:22948312]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0042645
mitochondrial nucleoid [PMID:10869431|PMID:10209025]"
/experiment="EXISTENCE:genetic interaction:GO:0036297
interstrand cross-link repair [PMID:22912599]"
/experiment="EXISTENCE:mutant phenotype:GO:0000725
recombinational repair [PMID:22027892]"
/experiment="EXISTENCE:mutant phenotype:GO:0003697
single-stranded DNA binding [PMID:22948312]"
/experiment="EXISTENCE:mutant phenotype:GO:0006281 DNA
repair [PMID:10209025]"
/note="Protein with a role in mitochondrial DNA
recombinational repair; also involved in interstrand
cross-link repair; binds to and catalyzes the annealing of
single-stranded mtDNA; oligomerizes to form rings and
filaments; related to Rad52-type recombination proteins,
with limited overall similarity but sharing conserved
functionally important residues; component of the
mitochondrial nucleoid, required for the repair of
oxidative mtDNA damage and mitochondrial genome
maintenance"
/codon_start=1
/product="Mgm101p"
/protein_id="NP_012678.1"
/db_xref="GeneID:853609"
/db_xref="SGD:S000003905"
/translation="MKSIFKVRGCVSHAAQFCQKRTVVSTGTSNTATAGAVRKSFNST
ETKPVFATKSEAGNGSHMKEYSSGINSKLGGTPLETRSTADDSLNNSYKQVKGDIDWY
TSWYGLGMKPFEAKVQKDLIEPLDPKDIEIKPDGLIYLPEIKYRRILNKAFGAGGWGL
VPRSQTIVTSKLVTREYGLICHGQLISVARGEQDYFNEAGIPTATEGCKSNALMRCCK
DLGVGSELWDPVFIKKFKVDHCTEKFVEHVTTKRKKKIWLRKDRQVEYPYK"
gene complement(<702027..>703068)
/gene="RPS4A"
/locus_tag="YJR145C"
/db_xref="GeneID:853610"
mRNA complement(join(<702027..702798,703055..>703068))
/gene="RPS4A"
/locus_tag="YJR145C"
/product="40S ribosomal protein eS4 RPS4A"
/transcript_id="NM_001181803.3"
/db_xref="GeneID:853610"
CDS complement(join(702027..702798,703055..703068))
/gene="RPS4A"
/locus_tag="YJR145C"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:6814480]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:6814480]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0022627 cytosolic
small ribosomal subunit [PMID:6814480]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/note="Protein component of the small (40S) ribosomal
subunit; mutation affects 20S pre-rRNA processing;
homologous to mammalian ribosomal protein S4, no bacterial
homolog; RPS4A has a paralog, RPS4B, that arose from the
whole genome duplication"
/codon_start=1
/product="40S ribosomal protein eS4 RPS4A"
/protein_id="NP_012679.3"
/db_xref="GeneID:853610"
/db_xref="SGD:S000003906"
/translation="MARGPKKHLKRLAAPHHWLLDKLSGCYAPRPSAGPHKLRESLPL
IVFLRNRLKYALNGREVKAILMQRHVKVDGKVRTDTTYPAGFMDVITLDATNENFRLV
YDVKGRFAVHRITDEEASYKLGKVKKVQLGKKGVPYVVTHDGRTIRYPDPNIKVNDTV
KIDLASGKITDFIKFDAGKLVYVTGGRNLGRIGTIVHKERHDGGFDLVHIKDSLDNTF
VTRLNNVFVIGEQGKPYISLPKGKGIKLSIAEERDRRRAQQGL"
gene <703885..>704238
/locus_tag="YJR146W"
/db_xref="GeneID:853611"
mRNA <703885..>704238
/locus_tag="YJR146W"
/product="uncharacterized protein"
/transcript_id="NM_001270754.1"
/db_xref="GeneID:853611"
CDS 703885..704238
/locus_tag="YJR146W"
/note="hypothetical protein; expressed at both mRNA and
protein levels; partially overlaps HMS2"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001257683.1"
/db_xref="GeneID:853611"
/db_xref="SGD:S000003907"
/translation="MKVGKAKKKPEIFSAHCSVATAFLDPSFFYPNFVAQKASHYNDK
TGSANIWTYISRTGSLLLFTQVVYRKSKWTHQSATFADCIYCQSGQSPSVSLSCSDAR
QTWMQHRGWSSLMSL"
gene <704196..>705272
/gene="HMS2"
/locus_tag="YJR147W"
/db_xref="GeneID:853612"
mRNA <704196..>705272
/gene="HMS2"
/locus_tag="YJR147W"
/product="Hms2p"
/transcript_id="NM_001181805.1"
/db_xref="GeneID:853612"
CDS 704196..705272
/gene="HMS2"
/locus_tag="YJR147W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0007124
pseudohyphal growth [PMID:9832522]"
/note="Protein with similarity to heat shock transcription
factors; overexpression suppresses the pseudohyphal
filamentation defect of a diploid mep1 mep2 homozygous
null mutant; HMS2 has a paralog, SKN7, that arose from the
whole genome duplication"
/codon_start=1
/product="Hms2p"
/protein_id="NP_012681.1"
/db_xref="GeneID:853612"
/db_xref="SGD:S000003908"
/translation="MDATSRMEQPDVFVSKLYHLLQGNAYSNIIQWSTDGSKLVIWNP
DQFTKVILERFFGIHTFAAFVKQLSKYNFQKAGRPDCVEFSNIHFQKDNINSLSLVKA
HQSAATPNVAAVNNMNKQCTFHWDPFKVNSILSKAIGKPSFEKLVKNVDRLQGNLDEL
KSTNADSLRIIREINASLQTISYHQFHAYQTANFLQENFEAIKKVVCPDSCLQHQQRQ
PKRPKRYSLLLLIPNASELSETPLMRFAGVFEFMNCSLDTATQWHPQLHPEAYDLLFV
TVSPNMQQEHLIYFKRLRNLLPSFPVIAIINRPVSPQDTSIAPSNYSRYYFHHFLQLG
FSDILVSPFTPTQLITLLSKHLRT"
gene <705744..>706874
/gene="BAT2"
/locus_tag="YJR148W"
/gene_synonym="ECA40; TWT2"
/db_xref="GeneID:853613"
mRNA <705744..>706874
/gene="BAT2"
/locus_tag="YJR148W"
/gene_synonym="ECA40; TWT2"
/product="branched-chain-amino-acid transaminase BAT2"
/transcript_id="NM_001181806.1"
/db_xref="GeneID:853613"
CDS 705744..706874
/gene="BAT2"
/locus_tag="YJR148W"
/gene_synonym="ECA40; TWT2"
/EC_number="2.6.1.42"
/experiment="EXISTENCE:direct assay:GO:0004084
branched-chain-amino-acid transaminase activity
[PMID:8798704]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276|PMID:14562095|PMID:21267457]"
/experiment="EXISTENCE:direct assay:GO:0009082
branched-chain amino acid biosynthetic process
[PMID:8798704]"
/experiment="EXISTENCE:mutant phenotype:GO:0009082
branched-chain amino acid biosynthetic process
[PMID:8798704]"
/experiment="EXISTENCE:mutant phenotype:GO:0009083
branched-chain amino acid catabolic process
[PMID:8702755]"
/note="Cytosolic branched-chain amino acid (BCAA)
aminotransferase; preferentially involved in BCAA
catabolism; homolog of murine ECA39; highly expressed
during stationary phase and repressed during logarithmic
phase; BAT2 has a paralog, BAT1, that arose from the whole
genome duplication"
/codon_start=1
/product="branched-chain-amino-acid transaminase BAT2"
/protein_id="NP_012682.1"
/db_xref="GeneID:853613"
/db_xref="SGD:S000003909"
/translation="MTLAPLDASKVKITTTQHASKPKPNSELVFGKSFTDHMLTAEWT
AEKGWGTPEIKPYQNLSLDPSAVVFHYAFELFEGMKAYRTVDNKITMFRPDMNMKRMN
KSAQRICLPTFDPEELITLIGKLIQQDKCLVPEGKGYSLYIRPTLIGTTAGLGVSTPD
RALLYVICCPVGPYYKTGFKAVRLEATDYATRAWPGGCGDKKLGANYAPCVLPQLQAA
SRGYQQNLWLFGPNNNITEVGTMNAFFVFKDSKTGKKELVTAPLDGTILEGVTRDSIL
NLAKERLEPSEWTISERYFTIGEVTERSKNGELLEAFGSGTAAIVSPIKEIGWKGEQI
NIPLLPGEQTGPLAKEVAQWINGIQYGETEHGNWSRVVTDLN"
gene <707160..>708374
/locus_tag="YJR149W"
/db_xref="GeneID:853614"
mRNA <707160..>708374
/locus_tag="YJR149W"
/product="putative nitronate monooxygenase"
/transcript_id="NM_001181807.4"
/db_xref="GeneID:853614"
CDS 707160..708374
/locus_tag="YJR149W"
/EC_number="1.13.12.16"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to the cytoplasm"
/codon_start=1
/product="putative nitronate monooxygenase"
/protein_id="NP_012683.4"
/db_xref="GeneID:853614"
/db_xref="SGD:S000003910"
/translation="MYFLNQLIFQDVSVMSVDKREDMSRSFQKCLNLRYPIIQAPMAG
VTTIEMAAKACIAGAIASLPLSHLDFRKVNDIEKLKLMVSQFRDQVADESLEGNLNLN
FFCHDIVDKPTDLQTANWAKLYRKSMNVPIDMNEIKFDNGNVSFKAFEKENALQDFFQ
YLSDGFRPKIISFHFGHPSKSTIEYLQKIGILIFVTATSVREVRLLARLGINGIVCQG
YEAGGHRGNFLVNDPKDDENLSTVQLVKRTVDELAEMKNKGLIHATPFVIAAGGIMDS
KDISYMLSQQADAVQVGTAFLGCSESNASKNFSSPFTRETTTKMVNIISGKPARTIST
PFIEKVIANFQGEELPPYGYMYSAFKQVRKKYPELANFILAGQGFQNVQSGITTDKKI
ETMGARLKIDGK"
gene complement(<708811..>709707)
/gene="DAN1"
/locus_tag="YJR150C"
/gene_synonym="CCW13"
/db_xref="GeneID:853615"
mRNA complement(<708811..>709707)
/gene="DAN1"
/locus_tag="YJR150C"
/gene_synonym="CCW13"
/product="Dan1p"
/transcript_id="NM_001181808.4"
/db_xref="GeneID:853615"
CDS complement(708811..709707)
/gene="DAN1"
/locus_tag="YJR150C"
/gene_synonym="CCW13"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:10322008]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0015918 sterol
transport [PMID:12077145]"
/note="Cell wall mannoprotein; has similarity to Tir1p,
Tir2p, Tir3p, and Tir4p; expressed under anaerobic
conditions, completely repressed during aerobic growth"
/codon_start=1
/product="Dan1p"
/protein_id="NP_012684.4"
/db_xref="GeneID:853615"
/db_xref="SGD:S000003911"
/translation="MSRISILAVAAALVASATAASVTTTLSPYDERVNLIELAVYVSD
IGAHLSEYYAFQALHKTETYPPEIAKAVFAGGDFTTMLTGISGDEVTRMITGVPWYST
RLMGAISEALANEGIATAVPASTTEASSTSTSEASSAATESSSSSESSAETSSNAAST
QATVSSESSSAASTIASSAESSVASSVASSVASSASFANTTAPVSSTSSISVTPVVQN
GTDSTVTKTQASTVETTITSCSNNVCSTVTKPVSSKAQSTATSVTSSASRVIDVTTNG
ANKFNNGVFGAAAIAGAAALLL"
rep_origin 711896..712142
/note="ARS1022; Autonomously Replicating Sequence"
/db_xref="SGD:S000028458"
gene complement(<712255..>715740)
/gene="DAN4"
/locus_tag="YJR151C"
/db_xref="GeneID:853616"
mRNA complement(<712255..>715740)
/gene="DAN4"
/locus_tag="YJR151C"
/product="Dan4p"
/transcript_id="NM_001181809.2"
/db_xref="GeneID:853616"
CDS complement(712255..715740)
/gene="DAN4"
/locus_tag="YJR151C"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:10383953]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="Cell wall mannoprotein; has similarity to Tir1p,
Tir2p, Tir3p, and Tir4p; expressed under anaerobic
conditions, completely repressed during aerobic growth"
/codon_start=1
/product="Dan4p"
/protein_id="NP_012685.1"
/db_xref="GeneID:853616"
/db_xref="SGD:S000003912"
/translation="MVNISIVAGIVALATSAAAITATTTLSPYDERVNLIELAVYVSD
IRAHIFQYYSFRNHHKTETYPSEIAAAVFDYGDFTTRLTGISGDEVTRMITGVPWYST
RLKPAISSALSKDGIYTAIPTSTSTTTTKSSTSTTPTTTITSTTSTTSTTPTTSTTST
TPTTSTTSTTPTTSTTSTTPTTSTTSTTPTTSTTSTTPTTSTTSTTPTTSTTSTTPTT
STTSTTPTTSTTPTTSTTSTTSQTSTKSTTPTTSSTSTTPTTSTTPTTSTTSTAPTTS
TTSTTSTTSTISTAPTTSTTSSTFSTSSASASSVISTTATTSTTFASLTTPATSTAST
DHTTSSVSTTNAFTTSATTTTTSDTYISSSSPSQVTSSAEPTTVSEVTSSVEPTRSSQ
VTSSAEPTTVSEFTSSVEPTRSSQVTSSAEPTTVSEFTSSVEPTRSSQVTSSAEPTTV
SEFTSSVEPTRSSQVTSSAEPTTVSEFTSSVEPTRSSQVTSSAEPTTVSEFTSSVEPI
RSSQVTSSAEPTTVSEVTSSVEPIRSSQVTTTEPVSSFGSTFSEITSSAEPLSFSKAT
TSAESISSNQITISSELIVSSVITSSSEIPSSIEVLTSSGISSSVEPTSLVGPSSDES
ISSTESLSATSTFTSAVVSSSKAADFFTRSTVSAKSDVSGNSSTQSTTFFATPSTPLA
VSSTVVTSSTDSVSPNIPFSEISSSPESSTAITSTSTSFIAERTSSLYLSSSNMSSFT
LSTFTVSQSIVSSFSMEPTSSVASFASSSPLLVSSRSNCSDARSSNTISSGLFSTIEN
VRNATSTFTNLSTDEIVITSCKSSCTNEDSVLTKTQVSTVETTITSCSGGICTTLMSP
VTTINAKANTLTTTETSTVETTITTCPGGVCSTLTVPVTTITSEATTTATISCEDNEE
DITSTETELLTLETTITSCSGGICTTLMSPVTTINAKANTLTTTETSTVETTITTCSG
GVCSTLTVPVTTITSEATTTATISCEDNEEDVASTKTELLTMETTITSCSGGICTTLM
SPVSSFNSKATTSNNAESTIPKAIKVSCSAGACTTLTTVDAGISMFTRTGLSITQTTV
TNCSGGTCTMLTAPIATATSKVISPIPKASSATSIAHSSASYTVSINTNGAYNFDKDN
IFGTAIVAVVALLLL"
gene <717580..>717630
/locus_tag="YJR151W-A"
/db_xref="GeneID:1466471"
mRNA <717580..>717630
/locus_tag="YJR151W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184556.1"
/db_xref="GeneID:1466471"
CDS 717580..717630
/locus_tag="YJR151W-A"
/note="hypothetical protein; identified by fungal homology
and RT-PCR; predicted to have a role in transcription
based on computational 'guilt by association' analysis"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878108.1"
/db_xref="GeneID:1466471"
/db_xref="SGD:S000028557"
/translation="MLSLIFYLRFPSYIRG"
gene <719666..>721297
/gene="DAL5"
/locus_tag="YJR152W"
/gene_synonym="UREP1"
/db_xref="GeneID:853617"
mRNA <719666..>721297
/gene="DAL5"
/locus_tag="YJR152W"
/gene_synonym="UREP1"
/product="allantoate permease"
/transcript_id="NM_001181810.3"
/db_xref="GeneID:853617"
CDS 719666..721297
/gene="DAL5"
/locus_tag="YJR152W"
/gene_synonym="UREP1"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0042938
dipeptide transport [PMID:17693598]"
/experiment="EXISTENCE:genetic interaction:GO:0071916
dipeptide transmembrane transporter activity
[PMID:17693598]"
/experiment="EXISTENCE:mutant phenotype:GO:0005886 plasma
membrane [PMID:3275614]"
/experiment="EXISTENCE:mutant phenotype:GO:0015124
allantoate transmembrane transporter activity
[PMID:3275614]"
/experiment="EXISTENCE:mutant phenotype:GO:0015719
allantoate transport [PMID:3275614]"
/experiment="EXISTENCE:mutant phenotype:GO:0042938
dipeptide transport [PMID:17693598|PMID:16429164]"
/experiment="EXISTENCE:mutant phenotype:GO:0042939
tripeptide transport [PMID:16429164]"
/experiment="EXISTENCE:mutant phenotype:GO:0071916
dipeptide transmembrane transporter activity
[PMID:17693598|PMID:16429164]"
/note="Allantoate permease; ureidosuccinate permease; also
transports dipeptides, though with lower affinity than for
allantoate and ureidosuccinate; expression is constitutive
but sensitive to nitrogen catabolite repression"
/codon_start=1
/product="allantoate permease"
/protein_id="NP_012686.3"
/db_xref="GeneID:853617"
/db_xref="SGD:S000003913"
/translation="MSADASTNSNASLDEKNLNITSEAEIKNEDVTAEPVLSTVLSPN
GKIVYISDKVDEAMKLAEEAKEIEVTPEEDRKLRWKIDYCMFPLMCILYAVQFMDKIS
TSSAAVMGLRTDLKMHGDQYSWVTSAFYFGYLFMNLGPVQFIFQRTSHMSKMLAVFIV
IWGMLLALHAAPTVKYPSFIVLRVLLGCAESVVTPCFTIITAQYWKTEEQFTRVSIWF
GMNGLGSILINAIAYGVYIHQDSYAIKGWRTLFVITGVITIFIGILIFLWIPDDPSKA
RFLSKREKLMVVQRIRSNQQGFGNHEIKKYQIIEALKDVRTWLYFLFTVSSNIPNGGI
SSFMSILLNSDFGYSSKETLLMGLPTGAVELVGCPLFGILAVYAANKKIPFWKYKLSW
AIFAAVLALIASCMLGFATNSKKARLAGAYLWYISPVSFICVLSNISANSSGYSKKWT
VSSINLVAYAAANLAGPQTFIAKQAPKYHGAKVAMVVCYAVMIVLLSILLIVNLRENK
RRDKIAAERGFPEETENLEFSDLTDFENPNFRYTL"
gene <722815..>723900
/gene="PGU1"
/locus_tag="YJR153W"
/gene_synonym="PGL1; PSM1"
/db_xref="GeneID:853618"
mRNA <722815..>723900
/gene="PGU1"
/locus_tag="YJR153W"
/gene_synonym="PGL1; PSM1"
/product="endo-polygalacturonase"
/transcript_id="NM_001181811.3"
/db_xref="GeneID:853618"
CDS 722815..723900
/gene="PGU1"
/locus_tag="YJR153W"
/gene_synonym="PGL1; PSM1"
/EC_number="3.2.1.15"
/experiment="EXISTENCE:direct assay:GO:0004650
polygalacturonase activity [PMID:10650215]"
/experiment="EXISTENCE:direct assay:GO:0005576
extracellular region [PMID:11935221|PMID:10028181]"
/experiment="EXISTENCE:direct assay:GO:0045490 pectin
catabolic process [PMID:10028181]"
/note="Endo-polygalacturonase; pectolytic enzyme that
hydrolyzes the alpha-1,4-glycosidic bonds in the
rhamnogalacturonan chains in pectins"
/codon_start=1
/product="endo-polygalacturonase"
/protein_id="NP_012687.3"
/db_xref="GeneID:853618"
/db_xref="SGD:S000003914"
/translation="MISANSLLISTLCAFAIATPLSKRDSCTLTGSSLSSLSTVKKCS
SIVIKDLTVPAGQTLDLTGLSSGTTVTFEGTTTFQYKEWSGPLISISGSKISVVGASG
HTIDGQGAKWWDGLGDSGKVKPKFVKLALTGTSKVTGLNIKNAPHQVFSINKCSDLTI
SDITIDIRDGDSAGGHNTDGFDVGSSSNVLIQGCTVYNQDDCIAVNSGSTIKFMNNYC
YNGHGISVGSVGGRSDNTVNGFWAENNHVINSDNGLRIKTVEGATGTVTNVNFISNKI
SGIKSYGIVIEGDYLNSKTTGTATGGVPISNLVMKDITGSVNSTAKRVKILVKNATNW
QWSGVSITGGSSYSGCSGIPSGSGASC"
gene <725784..>726824
/locus_tag="YJR154W"
/db_xref="GeneID:853619"
mRNA <725784..>726824
/locus_tag="YJR154W"
/product="uncharacterized protein"
/transcript_id="NM_001181812.1"
/db_xref="GeneID:853619"
CDS 725784..726824
/locus_tag="YJR154W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to the cytoplasm"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_012688.1"
/db_xref="GeneID:853619"
/db_xref="SGD:S000003915"
/translation="MNTDSHNLSEPYNIGGQKYINMKKKEDLGVCQPGLTQKAFTVED
KFDYKAIIEKMEVYGLCVVKNFIETSRCDEILKEIEPHFYRYESWQGSPFPKETTVAT
RSVLHSSTVLKDVVCDRMFCDISKHFLNEENYFAAGKVINKCTSDIQLNSGIVYKVGA
GASDQGYHREDIVHHTTHQACERFQYGTETMVGLGVAFTDMNKENGSTRMIVGSHLWG
PHDSCGNFDKRMEFHVNVAKGDAVLFLGSLYHAASANRTSQDRVAGYFFMTKSYLKPE
ENLHLGTDLRVFKGLPLEALQLLGLGISEPFCGHIDYKSPGHLISSSLFENDIEKGYY
GETIRVNYGSTQ"
gene <727405..>728271
/gene="AAD10"
/locus_tag="YJR155W"
/db_xref="GeneID:853620"
mRNA <727405..>728271
/gene="AAD10"
/locus_tag="YJR155W"
/product="putative aryl-alcohol dehydrogenase"
/transcript_id="NM_001181813.1"
/db_xref="GeneID:853620"
CDS 727405..728271
/gene="AAD10"
/locus_tag="YJR155W"
/note="Putative aryl-alcohol dehydrogenase; similar to P.
chrysosporium aryl-alcohol dehydrogenase; mutational
analysis has not yet revealed a physiological role;
members of the AAD gene family comprise three pairs (AAD3
+ AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14) whose two genes
are more related to one another than to other members of
the family"
/codon_start=1
/product="putative aryl-alcohol dehydrogenase"
/protein_id="NP_012689.1"
/db_xref="GeneID:853620"
/db_xref="SGD:S000003916"
/translation="MASRKLRDQIVIATKFTTDYKGYDVGKGKSANFCGNHKRSLHVS
VRDSLRKLQTDWIDILYVHWWDYMSSIEEVMDSLHILVQQGKVLYLGVSDTPAWVVSA
ANYYATSHGKTPFSIYQGKWNVLNRDFERDIIPMARHFGMALAPWDVMGGGRFQSKKA
VEERKKKGEGLRTFFGTSEQTDMEVKISEALLKVAEEHGTESVTAIAIAYVRSKAKHV
FPLVGGRKIEHLKQNIEALSIKLTPEQIKYLESIVPFDVGFPTNFIGDDPAVTKKPSF
LTEMSAKISFED"
gene complement(<728574..>729596)
/gene="THI11"
/locus_tag="YJR156C"
/db_xref="GeneID:853621"
mRNA complement(<728574..>729596)
/gene="THI11"
/locus_tag="YJR156C"
/product="4-amino-5-hydroxymethyl-2-methylpyrimidine
phosphate synthase"
/transcript_id="NM_001181814.3"
/db_xref="GeneID:853621"
CDS complement(728574..729596)
/gene="THI11"
/locus_tag="YJR156C"
/experiment="EXISTENCE:genetic interaction:GO:0009228
thiamine biosynthetic process [PMID:12777485]"
/note="Protein involved in synthesis of the thiamine
precursor HMP; member of a subtelomeric gene family
including THI5, THI11, THI12, and THI13;
hydroxymethylpyrimidine is also known as HMP"
/codon_start=1
/product="4-amino-5-hydroxymethyl-2-methylpyrimidine
phosphate synthase"
/protein_id="NP_012690.3"
/db_xref="GeneID:853621"
/db_xref="SGD:S000003917"
/translation="MSTDKITFLLNWQPTPYHIPIFLAQTKGYFKEQGLDIAILEPTN
PSDVTELIGSGKVDMGLKAMIHTLAAKARGFPVTSVASLLDEPFTGVLYLKGSGITED
FQSLKGKKIGYVGEFGKIQIDELTKHYGMKPEDYTAVRCGMNVAKYIIEDKIDAGIGI
ECMQQVELEEYLAKQGRPASDAKMLRIDKLACLGCCCFCTVLYICNDEFLKKNPEKVR
KFLKAIKKATDYVLADPVKAWKEYIDFKPQLNNDLSYKQYQRCYAYFSSSLYNVHRDW
KKVTGYGKRLAILPPDYVSNYTNEYLSWPEPEEVSDPLEAQRLMAIHQEKCRQEGTFK
RLALPA"
rep_origin 729983..730513
/note="ARS1023; Autonomously Replicating Sequence"
/db_xref="SGD:S000028459"
gene <732440..>734143
/gene="HXT16"
/locus_tag="YJR158W"
/gene_synonym="HLT3"
/db_xref="GeneID:853623"
mRNA <732440..>734143
/gene="HXT16"
/locus_tag="YJR158W"
/gene_synonym="HLT3"
/product="hexose transporter HXT16"
/transcript_id="NM_001181816.3"
/db_xref="GeneID:853623"
CDS 732440..734143
/gene="HXT16"
/locus_tag="YJR158W"
/gene_synonym="HLT3"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0015795
sorbitol transmembrane transport [PMID:26996892]"
/experiment="EXISTENCE:genetic interaction:GO:0015797
mannitol transmembrane transport [PMID:26996892]"
/experiment="EXISTENCE:mutant phenotype:GO:0005353
fructose transmembrane transporter activity
[PMID:10618490]"
/experiment="EXISTENCE:mutant phenotype:GO:0008645 hexose
transmembrane transport [PMID:10618490]"
/experiment="EXISTENCE:mutant phenotype:GO:0015578 mannose
transmembrane transporter activity [PMID:10618490]"
/experiment="EXISTENCE:mutant phenotype:GO:0055056
D-glucose transmembrane transporter activity
[PMID:10618490]"
/note="Putative transmembrane polyol transporter; supports
growth on and uptake of sorbitol with moderate affinity
and mannitol with lower affinity when overexpressed in a
strain deleted for hexose family members; minor hexose
transport activity when overexpressed in a similar strain;
similarity to hexose transporters; expression is repressed
by high levels of glucose"
/codon_start=1
/product="hexose transporter HXT16"
/protein_id="NP_012692.3"
/db_xref="GeneID:853623"
/db_xref="SGD:S000003919"
/translation="MASEQSSPEINADNLNSSAADVHVQPPGEKEWSDGFYDKEVING
NTPDAPKRGFLGYLIIYLLCYPVSFGGFLPGWDSGITAGFINMDNFKMNFGSYKHSTG
EYYLSNVRMGLLVAMFSVGCSIGGVAFARLADTLGRRLAIVIVVLVYMVGAIIQISSN
HKWYQYFVGKIIYGLGAGGCSVLCPMLLSEIAPTDLRGGLVSLYQLNMTFGIFLGYCS
VYGTRKYSNTAQWRIPVGLCFLWALIIIVGMLLVPESPRYLIECERHEEACVSIAKID
KVSPEDPWVLKQADEINAGVLAQRELGEASWKELFSVKTKVLQRLITGILVQTFLQLT
GENYFFFYGTTIFKSVGLTDGFETSIVLGTVNFFSTIIAVMVVDKIGRRKCLLFGAAS
MMACMVIFASIGVKCLYPHGQDGPSSKGAGNAMIVFTCFYIFCFATTWAPVAYIVVAE
SFPSKVKSKAMSISTAFNWLWQFLIGFFTPFITGSIHFYYGYVFVGCLVAMFLYVFFF
LPETIGLSLEETQLLYEEGIKPWKSASWVPPSRRGASSRETEAKKKSWKEVLKFPKSF
N"
gene <736044..>737117
/gene="SOR1"
/locus_tag="YJR159W"
/gene_synonym="SDH1"
/db_xref="GeneID:853624"
mRNA <736044..>737117
/gene="SOR1"
/locus_tag="YJR159W"
/gene_synonym="SDH1"
/product="L-iditol 2-dehydrogenase SOR1"
/transcript_id="NM_001181817.1"
/db_xref="GeneID:853624"
CDS 736044..737117
/gene="SOR1"
/locus_tag="YJR159W"
/gene_synonym="SDH1"
/EC_number="1.1.1.9"
/experiment="EXISTENCE:direct assay:GO:0003939 L-iditol
2-dehydrogenase (NAD+) activity [PMID:8125328]"
/experiment="EXISTENCE:expression pattern:GO:0019318
hexose metabolic process [PMID:8125328]"
/experiment="EXISTENCE:mutant phenotype:GO:0003939
L-iditol 2-dehydrogenase (NAD+) activity [PMID:26996892]"
/note="Sorbitol dehydrogenase; protein sequence is 99%
identical to the Sor2p sorbitol dehydrogenase; expression
is induced in the presence of sorbitol or xylose"
/codon_start=1
/product="L-iditol 2-dehydrogenase SOR1"
/protein_id="NP_012693.1"
/db_xref="GeneID:853624"
/db_xref="SGD:S000003920"
/translation="MSQNSNPAVVLEKVGDIAIEQRPIPTIKDPHYVKLAIKATGICG
SDIHYYRSGGIGKYILKAPMVLGHESSGQVVEVGDAVTRVKVGDRVAIEPGVPSRYSD
ETKEGRYNLCPHMAFAATPPIDGTLVKYYLSPEDFLVKLPEGVSYEEGACVEPLSVGV
HSNKLAGVRFGTKVVVFGAGPVGLLTGAVARAFGATDVIFVDVFDNKLQRAKDFGATN
TFNSSQFSTDKAQDLADGVQKLLGGNHADVVFECSGADVCIDAAVKTTKVGGTMVQVG
MGKNYTNFPIAEVSGKEMKLIGCFRYSFGDYRDAVNLVATGKVNVKPLITHKFKFEDA
AKAYDYNIAHGGEVVKTIIFGPE"
rep_origin 737034..737275
/note="ARS1024; Autonomously Replicating Sequence"
/db_xref="SGD:S000028460"
gene complement(<738008..>739816)
/gene="MPH3"
/locus_tag="YJR160C"
/db_xref="GeneID:853625"
mRNA complement(<738008..>739816)
/gene="MPH3"
/locus_tag="YJR160C"
/product="alpha-glucoside permease"
/transcript_id="NM_001181818.1"
/db_xref="GeneID:853625"
CDS complement(738008..739816)
/gene="MPH3"
/locus_tag="YJR160C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0008643
carbohydrate transport [PMID:12210897]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="Alpha-glucoside permease; transports maltose,
maltotriose, alpha-methylglucoside, and turanose;
identical to Mph2p; encoded in a subtelomeric position in
a region likely to have undergone duplication"
/codon_start=1
/product="alpha-glucoside permease"
/protein_id="NP_012694.1"
/db_xref="GeneID:853625"
/db_xref="SGD:S000003921"
/translation="MKNLSFLINRRKENTSDSNVYPGKAKSHEPSWIEMDDQTKKDGL
DIVHVEFSPDTRAPSDSNKVITEIFDATEDAKEADESERGMPLATALNTYPKAAAWSL
LVSTTLIMEGYDTAILGAFYALPIFQRKFGSQNDKTGEWEISASWQIGLTLCYMAGEI
VGLQLTGPSVDLVGNRYTLIIALFFLAAFTFILYFCNSLGMIAVGQALCGMPWGCFQC
LTVSYASEICPLALRYYLTTYSNLCWLFGQLFAAGIMKNSQKKYADSELGYKLPFALQ
WILPVPLALGIFFAPESPWWLVKKGRFDEARRSLRRTLSGKGPEKEILVTLEVDKIKV
TIDKEKRLTSKEGSYSDCFEDKINRRRTRITCLCWAGQATCGSILIGYSTYFYEKAGV
STEMSFTFSIIQYCLGICATFLSWWASKYFGRYDLYAFGLAFQTIVFFIIGGLGCSST
HGSKMGSGSLLMAVAFFYNLGIAPVVFCLVSEMPSSRLRTKTIILARNTYNVVSIICS
VLILYQLNSKKWNWGAKSGFFWGVLCFCTLIWAVVDLPETAGKTFVEINELFKLGVSA
RKFKSTKVDPFVVKTPPKDVSHNDPKGDIEASIAEE"
gene complement(<742848..>743999)
/gene="COS5"
/locus_tag="YJR161C"
/db_xref="GeneID:853626"
mRNA complement(<742848..>743999)
/gene="COS5"
/locus_tag="YJR161C"
/product="Cos5p"
/transcript_id="NM_001181819.3"
/db_xref="GeneID:853626"
CDS complement(742848..743999)
/gene="COS5"
/locus_tag="YJR161C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:25942624]"
/experiment="EXISTENCE:mutant phenotype:GO:0043328 protein
transport to vacuole involved in ubiquitin-dependent
protein catabolic process via the multivesicular body
sorting pathway [PMID:25942624]"
/note="Endosomal protein involved in turnover of plasma
membrane proteins; member of the DUP380 subfamily of
conserved, often subtelomeric COS genes; required for the
multivesicular vesicle body sorting pathway that
internalizes plasma membrane proteins for degradation; Cos
proteins provide ubiquitin in trans for nonubiquitinated
cargo proteins"
/codon_start=1
/product="Cos5p"
/protein_id="NP_012695.3"
/db_xref="GeneID:853626"
/db_xref="SGD:S000003922"
/translation="MKENELKNEKSVDVLSFKQLESQKIVLPQDLFRSSFTWFCYEIY
KSLAFPIWMLLWLPLSVWWKLSNNCIYPLIVSLLVLFLGPIFVLVICGLSRKRSLSKQ
LIQFCKEVTENTPSSDPHDWEVVAANLNSYLYENKAWNTRYFFFNAMGCQEAFRTTLL
EPFSLKKDEAAKVKSFKDSVPYIEEALGVYFREVEKQWKLFNTEKSWSPVGLEDVQLP
KDIHRSKLTWFLKRIFTIYSLPLWLAFLNCICVSQHFCLAFRILCPGLFFLMMVWLFQ
NMRTTALLVKMEHKMQFLLTIINEQESGANGWDEIARKMNRYLFEKKAWKNEEFFFEG
IDCEWFFSHFFYRLLSAKKSMWLLPLNVELWPYIKEAQLSRNEESLMKK"
rep_origin 744408..745008
/note="ARS1025; Autonomously Replicating Sequence"
/db_xref="SGD:S000028461"
telomere 744902..745751
/note="TEL10R; Telomeric region on the right arm of
Chromosome X; composed of an X element core sequence, X
element combinatorial repeats, and a short terminal
stretch of telomeric repeats"
/db_xref="SGD:S000028974"
CONTIG join(BK006943.2:1..745751)
//
