LOCUS NC_001140 562643 bp DNA linear CON 23-JAN-2026
DEFINITION Saccharomyces cerevisiae S288C chromosome VIII, complete sequence.
ACCESSION NC_001140
VERSION NC_001140.6
DBLINK BioProject: PRJNA128
Assembly: GCF_000146045.2
KEYWORDS RefSeq.
SOURCE Saccharomyces cerevisiae S288C
ORGANISM Saccharomyces cerevisiae S288C
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
Saccharomyces.
REFERENCE 1 (bases 1 to 562643)
AUTHORS Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M.,
Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S.,
Weng,S. and Cherry,J.M.
TITLE New data and collaborations at the Saccharomyces Genome Database:
updated reference genome, alleles, and the Alliance of Genome
Resources
JOURNAL Genetics 220 (4) (2022)
PUBMED 34897464
REFERENCE 2 (bases 1 to 562643)
AUTHORS Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
Oliver,S.G.
TITLE Life with 6000 genes
JOURNAL Science 274 (5287), 546 (1996)
PUBMED 8849441
REFERENCE 3 (bases 1 to 562643)
AUTHORS Johnston,M., Andrews,S., Brinkman,R., Cooper,J., Ding,H., Dover,J.,
Du,Z., Favello,A., Fulton,L., Gattung,S. et al.
TITLE Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
VIII
JOURNAL Science 265 (5181), 2077-2082 (1994)
PUBMED 8091229
REFERENCE 4 (bases 1 to 562643)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (23-JAN-2026) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 5 (bases 1 to 562643)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (04-MAY-2012) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Protein update by submitter
REFERENCE 6 (bases 1 to 562643)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (31-MAR-2011) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Sequence update by submitter
REFERENCE 7 (bases 1 to 562643)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (11-DEC-2009) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
COMMENT REVIEWED REFSEQ: This record has been curated by SGD. The reference
sequence is identical to BK006934.
On Apr 26, 2011 this sequence version replaced NC_001140.5.
##Genome-Annotation-Data-START##
Annotation Provider :: SGD
Annotation Status :: Full Annotation
Annotation Version :: R64-4-1
URL :: http://www.yeastgenome.org/
##Genome-Annotation-Data-END##
COMPLETENESS: full length.
FEATURES Location/Qualifiers
source 1..562643
/organism="Saccharomyces cerevisiae S288C"
/mol_type="genomic DNA"
/strain="S288C"
/db_xref="taxon:559292"
/chromosome="VIII"
telomere complement(1..5505)
/note="TEL08L; Telomeric region on the left arm of
Chromosome VIII; composed of an X element core sequence,
an X element combinatorial repeat, a short Y' element, and
a short terminal stretch of telomeric repeats"
/db_xref="SGD:S000028891"
gene complement(<445..>3311)
/locus_tag="YHL050C"
/db_xref="GeneID:856335"
mRNA complement(join(<445..1897,2671..>3311))
/locus_tag="YHL050C"
/product="uncharacterized protein"
/transcript_id="NM_001179130.1"
/db_xref="GeneID:856335"
CDS complement(join(445..1897,2671..3311))
/locus_tag="YHL050C"
/note="hypothetical protein; potential Cdc28p substrate"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011813.1"
/db_xref="GeneID:856335"
/db_xref="SGD:S000001042"
/translation="MADTPSVAVQAPPGYGKTELFHLPLIALASKGDVEYVSFLFVPY
TVLLANCMIRLGRCGCLNVAPVRNFIEEGCDGVTDLYVGIYDDLASTNFTDRIAAWEN
IVECTFRTNNVKLGYLIVDELHNFETEVYRQSQFGGITNLDFDAFEKAIFLSGTAPEA
VADAALQRIGLTGLAKKSMDINELKRSEDLSRGLSSYPTRMFNLIKEKSKVPLGTNAT
TTASTNVRTSATTTASINVRTSATTTASINVRTSATTTESTNSNTNATTTESTNSSTN
ATTTASTNSSTNATTTESTNASAKEDANKDGNAEDNRFHPVTDINKEPYKRKGSQMVL
LERKKLKAQFPNTSENMNVLQFLGFRSDEIKHLFLYGIDIYFCPEGVFTQYGLCKGCQ
KMFELCVCWAGQKVSYRRMAWEALAVERMLRNDEEYKEYLEDIEPYHGDPVGYLKFFS
VKRREIYSQIQRNYAWYLAITRRRETISVLDSTRGKQGSQVFRMSGRQIKELYYKVWS
NLRESKTEVLQYFLNWDEKKCQEEWEAKDDTVFVEALEKVGVFQRLRSMTSAGLQGPQ
YVKLQFSRHHRQLRSRYELSLGMHLRDQLALGVTPSKVPHWTAFLSMLIGLFYNKTFR
QKLEYLLEQISEVWLLPHWLDLANVEVLAADNTRVPLYMLMVAVHKELDSDDVPDGRF
DIILLCRDSSREVGE"
gene complement(<3726..>4541)
/locus_tag="YHL049C"
/db_xref="GeneID:856336"
mRNA complement(<3726..>4541)
/locus_tag="YHL049C"
/product="uncharacterized protein"
/transcript_id="NM_001179129.1"
/db_xref="GeneID:856336"
CDS complement(3726..4541)
/locus_tag="YHL049C"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011814.1"
/db_xref="GeneID:856336"
/db_xref="SGD:S000001041"
/translation="MKVSDRRKFEKANFDEFESALNNKNDLVHCPSITLFESIPTEVR
SFYEDEKSGLIKVVKFRTGAMDRKRSFEKIVVSVMVGKNVQKFLTFVEDEPDFQGGPI
PSKYLIPKKINLMVYTLFQVHTLKFNRKDYDTLSLFYLNRGYYNELSFRVLERCHEIA
SARPNDSSTMRTFTDFVSGAPIVRSLQKSTIRKYGYNLAPHMFLLLHVDELSIFSAYQ
ASLPGEKKVDTERLKRDLCPRKPTEIKYFSQICNDMMNKKDRLGDVLHVCCPS"
rep_origin 5471..6401
/note="ARS801; Putative replication origin; identified in
multiple array studies, not yet confirmed by plasmid-based
assay"
/db_xref="SGD:S000130145"
gene complement(<5663..>5797)
/locus_tag="YHL048C-A"
/db_xref="GeneID:1466524"
mRNA complement(<5663..>5797)
/locus_tag="YHL048C-A"
/product="uncharacterized protein"
/transcript_id="NM_001184650.1"
/db_xref="GeneID:1466524"
CDS complement(5663..5797)
/locus_tag="YHL048C-A"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:35563734]"
/note="hypothetical protein; identified by expression
profiling and mass spectrometry; localizes to the
peroxisome in a Pex5p-dependent manner in oleate"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878084.1"
/db_xref="GeneID:1466524"
/db_xref="SGD:S000028829"
/translation="MHDIWVITTSPACFEILYKYCKQKGRARMGGLIVKIIRFNHASV
"
gene <6401..>7546
/gene="COS8"
/locus_tag="YHL048W"
/db_xref="GeneID:856337"
mRNA <6401..>7546
/gene="COS8"
/locus_tag="YHL048W"
/product="Cos8p"
/transcript_id="NM_001179128.1"
/db_xref="GeneID:856337"
CDS 6401..7546
/gene="COS8"
/locus_tag="YHL048W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:12051917]"
/note="Endosomal protein involved in turnover of plasma
membrane proteins; member of the DUP380 subfamily of
conserved, often subtelomeric COS genes; required for the
multivesicular vesicle body sorting pathway that
internalizes plasma membrane proteins for degradation; Cos
proteins provide ubiquitin in trans for nonubiquitinated
cargo proteins"
/codon_start=1
/product="Cos8p"
/protein_id="NP_011815.1"
/db_xref="GeneID:856337"
/db_xref="SGD:S000001040"
/translation="MKENEVKDEKSVDVLSFKQLEFQKTVLPQDVFRNELTWFCYEIY
KSLAFRIWMLLWLPLSVWWKLSSNWIHPLIVSLLVLFLGPFFVLVICGLSRKRSLSKQ
LIQFCKEITEDTPSSDPHDWEVVAANLNSYFYENKTWNTKYFFFNAMSCQKAFKTTLL
EPFSLKKDESAKVKSFKDSVPYIEEALQVYAAGFDKEWKLFNTEKEESPFDLEDIQLP
KEAYRFKLTWILKRIFNLRCLPLFLYYFLIVYTSGNADLISRFLFPVVMFFIMTRDFQ
NMRMIVLSVKMEHKMQFLSTIINEQESGANGWDEIAKKMNRYLFEKKVWNNEEFFYDG
LDCEWFFRRFFYRLLSLKKPMWFASLNVELWPYIKEAQSARNEKPLK"
rep_origin 7702..7767
/note="ARS802; Autonomously Replicating Sequence"
/db_xref="SGD:S000118356"
repeat_region complement(7994..8225)
/note="Ty5 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006986"
gene complement(<8351..>10213)
/gene="ARN2"
/locus_tag="YHL047C"
/gene_synonym="TAF1"
/db_xref="GeneID:856338"
mRNA complement(<8351..>10213)
/gene="ARN2"
/locus_tag="YHL047C"
/gene_synonym="TAF1"
/product="siderophore transporter"
/transcript_id="NM_001179127.2"
/db_xref="GeneID:856338"
CDS complement(8351..10213)
/gene="ARN2"
/locus_tag="YHL047C"
/gene_synonym="TAF1"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0015343
siderophore-iron transmembrane transporter activity
[PMID:10816729]"
/experiment="EXISTENCE:direct assay:GO:0015891 siderophore
transport [PMID:10816729]"
/experiment="EXISTENCE:mutant phenotype:GO:0006879
intracellular iron ion homeostasis [PMID:10816729]"
/experiment="EXISTENCE:mutant phenotype:GO:0009237
siderophore metabolic process [PMID:10816729]"
/experiment="EXISTENCE:mutant phenotype:GO:0015343
siderophore-iron transmembrane transporter activity
[PMID:10748025|PMID:10816729]"
/experiment="EXISTENCE:mutant phenotype:GO:0015891
siderophore transport [PMID:10816729]"
/note="Transporter; member of the ARN family of
transporters that specifically recognize siderophore-iron
chelates; responsible for uptake of iron bound to the
siderophore triacetylfusarinine C"
/codon_start=1
/product="siderophore transporter"
/protein_id="NP_011816.2"
/db_xref="GeneID:856338"
/db_xref="SGD:S000001039"
/translation="MIEVPEDNRSSQTKRKNTEKNCNELMVDEKMDDDSSPRDEMKDK
LKGTKSLIIRKSELMAKKYDTWQLKAIFLFSAFICTFAYGLDSSIRGTYMTYAMNSYS
AHSLISTVSVIVLMISAVSQVIFGGLSDIFGRLTLFLVSIVLYIVGTIIQSQAYDVQR
YAAGAVFYYVGLVGVMLQVVLMLSDNSSLKWRLFYTLIPSWPSIITTWVSGSVVEAAN
PLENWSWNIAMWAFIFPLCCIPLILCMLHMRWKVRNDVEWKELQDEKSYYQTHGLVQM
LVQLFWKLDVVGVLLFTAGVGCILVPLTLAGGVSTNWRNSKIIGPFVLGFVLVPGFIY
WESRLALVPFAPFKLLKDRGVWAPLGIMFFICFVYQMAAGYLYTILVVAVDESASSAT
RIINLYSFVTAVVAPFLGLIVTRSSRLKSYIIFGGSLYFITMGLFYRYRSGQDADGGI
IAGMVIWGLSSCLFDYPTIVSIQSVTSHENMATVTALNYTVFRIGGAVAAAISGAIWT
QSLYPKLLHYMGDADLATAAYGSPLTFILSNPWGTPVRSAMVEAYRHVQKYEVIVALV
FSAPMFLLTFCVRDPRLTEDFAQKLPDREYVQTKEDDPINDWIAKRFAKALGRS"
gene complement(<11923..>12285)
/gene="PAU13"
/locus_tag="YHL046C"
/db_xref="GeneID:856339"
mRNA complement(<11923..>12285)
/gene="PAU13"
/locus_tag="YHL046C"
/product="seripauperin PAU13"
/transcript_id="NM_001179126.1"
/db_xref="GeneID:856339"
CDS complement(11923..12285)
/gene="PAU13"
/locus_tag="YHL046C"
/note="hypothetical protein; member of the seripauperin
multigene family encoded mainly in subtelomeric regions;
expression is induced after ethanol shock; SWAT-GFP and
mCherry fusion proteins localize to the endoplasmic
reticulum and vacuole respectively"
/codon_start=1
/product="seripauperin PAU13"
/protein_id="NP_011817.1"
/db_xref="GeneID:856339"
/db_xref="SGD:S000001038"
/translation="MVKLTSIAAGVAAIAATASATTTLAQSDERVNLVELGVYVSDIR
AHLAQYYMFQAAHPTETYPVEVAEAVFNYGDFTTMLTGIAPDQVTRMITGVPWYSSRL
KPAISSALSKDGIYTITN"
gene <12502..>12849
/gene="PXP3"
/locus_tag="YHL045W"
/db_xref="GeneID:856340"
mRNA <12502..>12849
/gene="PXP3"
/locus_tag="YHL045W"
/product="Pxp3p"
/transcript_id="NM_001416099.1"
/db_xref="GeneID:856340"
CDS 12502..12849
/gene="PXP3"
/locus_tag="YHL045W"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:36164978]"
/note="Peroxisomal hypothetical protein; last 10
C-terminal amino acids contains a novel PTS1 motif, both
necessary and sufficient for Pex5p-dependent peroxisomal
targeting; also localizes to the nucleus; PXP3 is not an
essential gene"
/codon_start=1
/product="Pxp3p"
/protein_id="NP_001403028.1"
/db_xref="GeneID:856340"
/db_xref="SGD:S000001037"
/translation="MKMLLFLNEACIFIDSVCEGIVFWGLCLFVCAECGNAYYRGARV
PYKTLFRAFEVSVFGQKEYPNFRFGPSYRFLCLSPYSICCKQPPMEEVILYYPSPDSL
IKNRKRVLGVAYL"
gene <13565..>14272
/gene="DFP4"
/locus_tag="YHL044W"
/db_xref="GeneID:856341"
mRNA <13565..>14272
/gene="DFP4"
/locus_tag="YHL044W"
/product="DUP240 family protein"
/transcript_id="NM_001179124.1"
/db_xref="GeneID:856341"
CDS 13565..14272
/gene="DFP4"
/locus_tag="YHL044W"
/experiment="EXISTENCE:mutant phenotype:GO:0005886 plasma
membrane [PMID:12101299]"
/note="Putative integral membrane protein; member of
DUP240 gene family; green fluorescent protein (GFP)-fusion
protein localizes to the plasma membrane in a punctate
pattern"
/codon_start=1
/product="DUP240 family protein"
/protein_id="NP_011819.1"
/db_xref="GeneID:856341"
/db_xref="SGD:S000001036"
/translation="MSSELLISNSKPRPEGLRKLCEGETVILPRDITPSKCAYFLKQN
IVFISYIFIHIIITIILNRLALSAHGNTLIIILAALLITISLFLLLLLPYLSCSRYKL
RCLDDDCKFKLLAEVITHKPNMDLSTWDRIAYDMNQFVYDRRICADRSFFYDGSYCYQ
VFKKLVATPYLVNSNMNSIYADLEMRSNGATNINDSGNSSLHIELGTYIFKALAVFRN
SVDKYWEDKYPEMGVTV"
gene <14901..>15413
/gene="ECM34"
/locus_tag="YHL043W"
/db_xref="GeneID:856342"
mRNA <14901..>15413
/gene="ECM34"
/locus_tag="YHL043W"
/product="Ecm34p"
/transcript_id="NM_001179123.1"
/db_xref="GeneID:856342"
CDS 14901..15413
/gene="ECM34"
/locus_tag="YHL043W"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/note="hypothetical protein; member of the DUP380
subfamily of conserved, often subtelomerically-encoded
proteins; SWAT-GFP, seamless-GFP and mCherry C-terminal
fusion proteins localize to the cytosol"
/codon_start=1
/product="Ecm34p"
/protein_id="NP_011820.1"
/db_xref="GeneID:856342"
/db_xref="SGD:S000001035"
/translation="MEGRKSEDEKNEAALACDVFESSNAKLPKNVFRSSFTWYCYEVI
NRSAFHIWLLLCLTLIVGWKVFSGIGGRRPSDSNMDGPQTKHKRNPGFLRRHSTIVIL
VISLAVSFSWEAFKMYRERTFGKQITQFAKEIIKSAPSTDMESWDRVAADFNSYMYEN
KLWNTEYFFC"
gene <15667..>16119
/locus_tag="YHL042W"
/db_xref="GeneID:856343"
mRNA <15667..>16119
/locus_tag="YHL042W"
/product="uncharacterized protein"
/transcript_id="NM_001179122.1"
/db_xref="GeneID:856343"
CDS 15667..16119
/locus_tag="YHL042W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:31945465]"
/note="Endoplasmic reticulum protein; member of DUP380
subfamily of conserved, often subtelomerically-encoded
proteins; may contribute to maintenance of ER and nuclear
morphology; null mutants have distorted nuclear envelope
shape, and discontinuous, misshapen perinuclear ER"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011821.1"
/db_xref="GeneID:856343"
/db_xref="SGD:S000001034"
/translation="MKQRFSQVATVIFFVMSIRSPRNLGFFFTLALFVVLVCSQEWFS
FEMNRSCSMKVEHRMQFLSTIISEHQKSDVNCWDQIAKKMNVYLFEQKVSGSDVFFLD
GADCERFFERNFLRYLPSRKSSHPDLPIAELLPYIRKADIACAGKQLI"
gene <17392..>17841
/locus_tag="YHL041W"
/db_xref="GeneID:856344"
mRNA <17392..>17841
/locus_tag="YHL041W"
/product="uncharacterized protein"
/transcript_id="NM_001348807.1"
/db_xref="GeneID:856344"
CDS 17392..17841
/locus_tag="YHL041W"
/note="hypothetical protein; conserved across S.
cerevisiae strains"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335736.1"
/db_xref="GeneID:856344"
/db_xref="SGD:S000001033"
/translation="MHLYSIAKSTTILRFLRHILLPFLHLSIHFTSCLCRIIYVGWKS
FWKHFFFCGHKIKTPLFIASYAMFLAKQKIIVTMFEVSSVLEKLPQNILLFHYPLPSK
MGNNLLMSFLTQRSIDEKEIDALLHPNAVYRFCNMRTTFRTLSSYGK"
gene complement(<19088..>20971)
/gene="ARN1"
/locus_tag="YHL040C"
/db_xref="GeneID:856345"
mRNA complement(<19088..>20971)
/gene="ARN1"
/locus_tag="YHL040C"
/product="siderophore transporter"
/transcript_id="NM_001179120.1"
/db_xref="GeneID:856345"
CDS complement(19088..20971)
/gene="ARN1"
/locus_tag="YHL040C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:17344478]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:15719020]"
/experiment="EXISTENCE:direct assay:GO:0031410 cytoplasmic
vesicle [PMID:10748025]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0015343
siderophore-iron transmembrane transporter activity
[PMID:10802175]"
/experiment="EXISTENCE:mutant phenotype:GO:0015891
siderophore transport [PMID:10802175]"
/note="ARN family transporter for siderophore-iron
chelates; responsible for uptake of iron bound to
ferrirubin, ferrirhodin, and related siderophores; protein
increases in abundance and relocalizes to the vacuole upon
DNA replication stress"
/codon_start=1
/product="siderophore transporter"
/protein_id="NP_011823.1"
/db_xref="GeneID:856345"
/db_xref="SGD:S000001032"
/translation="MESVHSRDPVKEEKKHVFMGMEHELNPETHNDSNSDSYGLPQLS
EKYNALRQNRSLIIQQTEIIGSAYNKWYLQAILLLSAFICGYGYGLDGNIRYIYTGYA
TSSYSEHSLLSTINVINAVVSAASQIIYARLSDVFGRLYLFISAVILYVVGTIIQSQA
YDVQRYAAGAIFYNAGYVGVILILLIILSDFSSLKWRLLYQFVPTWPFIINTWIAGNI
TSRANPVVNWSWDVGMWAFIFPLSCVPIVLCMLHMQWRARKTPEWHALKGQKSYYQEH
GFIKILKQLFWMLDVVGVLLMGCSLGCILVPLTLAGGVKTTWNDSRLIGPFVLGFVLI
PILWIWEYRFARDPILPYRLVKDRAVWSSMGISFLIDFIYYMAADYLYTVMIVAVNES
VKSATRIATLSSFVSTVASPFFALLVTRCTRLKPFIMFGCALWMVAMGLLYHFRGGSQ
SHSGIIGALCVWGVGTTLFTYPVTVSVQSAVSHENMATVTALNYTLYRIGSAVGSAVS
GAIWTQTLYKQILKRMGDVALATTAYESPYTFIETYTWGTPQRNALMNAYKYVQRLET
IVALVFCVPLIAFSLCLRDPKLTDTVAVEYIEDGEYVDTKDNDPILDWFEKLPSKFTF
KRE"
gene <21783..>23540
/gene="EFM1"
/locus_tag="YHL039W"
/db_xref="GeneID:856346"
mRNA <21783..>23540
/gene="EFM1"
/locus_tag="YHL039W"
/product="protein-lysine N-methyltransferase"
/transcript_id="NM_001179119.1"
/db_xref="GeneID:856346"
CDS 21783..23540
/gene="EFM1"
/locus_tag="YHL039W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0016279
protein-lysine N-methyltransferase activity
[PMID:22522802]"
/experiment="EXISTENCE:mutant phenotype:GO:0018026
peptidyl-lysine monomethylation
[PMID:20510667|PMID:22522802|PMID:24517342]"
/note="Lysine methyltransferase; involved in the
monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family
member; predicted involvement in ribosome biogenesis;
green fluorescent protein (GFP)-fusion protein localizes
to the cytoplasm"
/codon_start=1
/product="protein-lysine N-methyltransferase"
/protein_id="NP_011824.1"
/db_xref="GeneID:856346"
/db_xref="SGD:S000001031"
/translation="MITQTELDNCLQWAQNNGAFIDPKISFRITEDAGVSAFVNEKFS
PKPDQALIRVPETLLITSQQALSEFSQAANERSLLNSVTQLYLSKLKFGTDAVHLKSF
YKPYLDVLPLHLPQPYFWSTDEVMNLHGTDVYLTMRDTLNKLVKEWRMLFQALSIEHS
SQDKQFLSLFQENKDSAVVPLEQFCAHINGCKLEDSEWNSFVAYLWSYCIFNSRAFPR
VILGRAGTDRTNLNEGFLYPIVDLLNHKNDVPVRWEMNEQNELCFMSQTTTFSAQDEL
FNNYGNISNEKCLLNYGFWDSSNKFDFSRLTLKLPSTLVSGLPVDFNKSGNFVTDDGE
TTILQFSLKISEPLPPVLLALFAYLSKLKSEETPTVRSVLEGIDQLTSVVSQRLLFYK
NFKIKTSSTQKLRPHVIKLIKLYYQDNKKILNATTEKLSVLQKKIYSNNKEFSLSFKT
IFKNDKIFANSLLLVFGAINYEDLITKDCLNDALLLWIVKLINDKSNNQGGFIKQTFK
EVSDSIVIEKEDVMEFLPFYKKYFPNLSERIPEIYSVGDWGIRQFIVADTAIDRLVWI
RKSNKEPIFLMKKAYDLQI"
gene complement(<23617..>25509)
/gene="CBP2"
/locus_tag="YHL038C"
/db_xref="GeneID:856347"
mRNA complement(<23617..>25509)
/gene="CBP2"
/locus_tag="YHL038C"
/product="Cbp2p"
/transcript_id="NM_001179118.1"
/db_xref="GeneID:856347"
CDS complement(23617..25509)
/gene="CBP2"
/locus_tag="YHL038C"
/experiment="EXISTENCE:direct assay:GO:0000372 Group I
intron splicing [PMID:9092668]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:10521416]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:2685564|PMID:24769239]"
/experiment="EXISTENCE:mutant phenotype:GO:0003723 RNA
binding [PMID:10521416]"
/experiment="EXISTENCE:mutant phenotype:GO:0090615
mitochondrial mRNA processing [PMID:6348045]"
/note="Required for splicing of the group I intron bI5 of
the COB pre-mRNA; nuclear-encoded mitochondrial protein
that binds to the RNA to promote splicing; also involved
in but not essential for splicing of the COB bI2 intron
and the intron in the 21S rRNA gene"
/codon_start=1
/product="Cbp2p"
/protein_id="NP_011825.1"
/db_xref="GeneID:856347"
/db_xref="SGD:S000001030"
/translation="MVNWQTLFMVSLRRQGSSSRYRYKFNMENITHQVFPRCKQAFKK
TNLSYEYCDLEGVLYNISLTDLQKLLLRDINAPREHAFKIVRTDLTQKSSKKRIQHWE
RIAPMFDHPLSLYEKLFSEMDEDFKPSFEWQQLIRVRCKDDKLKLQRVIWPKSIFSNF
CRGIGVKKSTYDRLLEQNNGEVPMFVNPANAKPLPLFQVSDEAMIGEFDGIGIFPYFV
DKHREFFVTEVDKLKTKIASPLCTLNERKRIEKANAGRILANEEGKPFYLDANSATTR
IAGGNVVTLKQLLERSVSHKTLWSKQSNKDRTCPGDILRATILSNDFSIRQLRAEFCK
NFILYNIFTILQRNKKSIRSFSGNDNAPSFQFSWNVWDSYIWKQYQETESMKIPTDQA
SLINYKTKYDSFLHDLQTYSALVISEMKWNQFSIFQNDETTLSRFEHITLILQTVLTK
SKMIRIFQPNLYKFMQDDLRPTLMELVGFTESINATIEPGFANEQSLQSANGLKKLAN
QLLYFEQEIYGEKFRVNRPIQLRPITLSANYKIVILDKKNAIPEIFQTLLKFMTQITT
YFVKDLSEVELHGHMHCIDKKMLDKSKFMYLYEEKSNEEVKAASPQKEKIVDNIIGLL
SNDEEH"
gene complement(<25778..>26179)
/locus_tag="YHL037C"
/db_xref="GeneID:856348"
mRNA complement(<25778..>26179)
/locus_tag="YHL037C"
/product="uncharacterized protein"
/transcript_id="NM_001348812.1"
/db_xref="GeneID:856348"
CDS complement(25778..26179)
/locus_tag="YHL037C"
/note="hypothetical protein; conserved among S. cerevisiae
strains"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335737.1"
/db_xref="GeneID:856348"
/db_xref="SGD:S000001029"
/translation="MYRSSISIQVFICVLFLPLDSGRPLIYMIDLSYICSDATVFSGK
SRVIFFSNPICEHTRGNFYLFYLNYIINTFAPKNRGTRRCKPFGLHINCRKKIDCLHV
QLSYILCKNKSRKRHMDAHAITLAWLAHALT"
gene <26241..>27881
/gene="MUP3"
/locus_tag="YHL036W"
/db_xref="GeneID:856349"
mRNA <26241..>27881
/gene="MUP3"
/locus_tag="YHL036W"
/product="Mup3p"
/transcript_id="NM_001179116.1"
/db_xref="GeneID:856349"
CDS 26241..27881
/gene="MUP3"
/locus_tag="YHL036W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0015191
L-methionine transmembrane transporter activity
[PMID:8893857]"
/experiment="EXISTENCE:genetic interaction:GO:1903692
methionine import across plasma membrane [PMID:8893857]"
/experiment="EXISTENCE:mutant phenotype:GO:0015191
L-methionine transmembrane transporter activity
[PMID:8893857]"
/experiment="EXISTENCE:mutant phenotype:GO:1903692
methionine import across plasma membrane [PMID:8893857]"
/note="Low affinity methionine permease; similar to Mup1p"
/codon_start=1
/product="Mup3p"
/protein_id="NP_011827.1"
/db_xref="GeneID:856349"
/db_xref="SGD:S000001028"
/translation="MEPLLFNSGKANPSQDVFIDVEVGDITTKYGSTNTGSFSSMDTV
EAQAIKAETARFMEVPQGRHLGVFSTVVLFVSRIMGSGIFAVPSVILLNTGGNKLIYF
AIWVFSAAIAFAGLYLFLEFGSWIPKSGGRKNFLERSFERPRLLISVVFSCYSVLTGY
ALTGSIVFGKYVLSAFGVTDDSWSKYVSISFIIFAVLIHGVSVRHGVFIQNALGGLKL
IMIVLMCFAGLYTLFFYKSTGQVAWDLPVTQVEKDSLLSVSSIATAFISSFFCFSGWD
TVHTVTSEIKNPVKTLKVSGPLSLIICFVCYTMMNVAYLKVLTYEEIVSAGPLVGSVL
FTKLFGPRVGGKFIAFSIAISAASNILVVIYSISRVNQEIFKEGYLPFSIHMSKNWPF
DAPLPSISLCGFITIAWILILPKEGESFNYLVSMDGYGNQFFLLLVAIGLFIWRFKHK
NEVPEIRASTFGVLAIITLSLYMLMAPFFADPSLNRVGFLPPYQIMSLLVIVACFFFW
LVKFVLLPKFFHYKLLPKITYLHDGLIVTEWVKKPCLC"
gene complement(<27978..>32756)
/gene="VMR1"
/locus_tag="YHL035C"
/db_xref="GeneID:856350"
mRNA complement(<27978..>32756)
/gene="VMR1"
/locus_tag="YHL035C"
/product="putative ATP-binding cassette multidrug
transporter VMR1"
/transcript_id="NM_001179115.1"
/db_xref="GeneID:856350"
CDS complement(27978..32756)
/gene="VMR1"
/locus_tag="YHL035C"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:23658021|PMID:20846144]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:genetic interaction:GO:1990961
xenobiotic detoxification by transmembrane export across
the plasma membrane [PMID:20846144]"
/experiment="EXISTENCE:mutant phenotype:GO:0010038
response to metal ion [PMID:20846144]"
/experiment="EXISTENCE:mutant phenotype:GO:1990961
xenobiotic detoxification by transmembrane export across
the plasma membrane [PMID:20846144]"
/note="Vacuolar membrane protein; involved in multiple
drug resistance and metal sensitivity; ATP-binding
cassette (ABC) family member involved in drug transport;
potential Cdc28p substrate; induced under respiratory
conditions; VMR1 has a paralog, YBT1, that arose from the
whole genome duplication"
/codon_start=1
/product="putative ATP-binding cassette multidrug
transporter VMR1"
/protein_id="NP_011828.1"
/db_xref="GeneID:856350"
/db_xref="SGD:S000001027"
/translation="MGTDPLIIRNNGSFWEVDDFTRLGRTQLLSYYLPLAIIASIGIF
ALCRSGLSRYVRSAECDLVNEYLFGAQEERKEDNSIERLLRNSNTQANYVNVKKQGRI
LKLRHFDITTIDVKQIDAKNHGGLTFSRPSTSDHLRKSSEIVLMSLQIIGLSFLRVTK
INIELTNRDVTTLLLFWLILLSLSILRVYKRSTNLWAICFTAHTTIWISTWIPIRSVY
IGNIDDVPSQIFYIFEFVITSTLQPIKLTSPIKDNSSIIYVRDDHTSPSREHISSILS
CITWSWITNFIWEAQKNTIKLKDIWGLSMEDYSIFILKGFTRRNKHINNLTLALFESF
KTYLLIGMLWVLVNSIVNLLPTILMKRFLEIVDNPNRSSSCMNLAWLYIIGMFICRLT
LAICNSQGQFVSDKICLRIRAILIGEIYAKGLRRRLFTSPKTSSDSDSISANLGTIIN
LISIDSFKVSELANYLYVTVQAVIMIIVVVGLLFNFLGVSAFAGISIILVMFPLNFLL
ANLLGKFQKQTLKCTDQRISKLNECLQNIRIVKYFAWERNIINEIKSIRQKELRSLLK
KSLVWSVTSFLWFVTPTLVTGVTFAICTFVQHEDLNAPLAFTTLSLFTLLKTPLDQLS
NMLSFINQSKVSLKRISDFLRMDDTEKYNQLTISPDKNKIEFKNATLTWNENDSDMNA
FKLCGLNIKFQIGKLNLILGSTGSGKSALLLGLLGELNLISGSIIVPSLEPKHDLIPD
CEGLTNSFAYCSQSAWLLNDTVKNNIIFDNFYNEDRYNKVIDACGLKRDLEILPAGDL
TEIGEKGITLSGGQKQRISLARAVYSSAKHVLLDDCLSAVDSHTAVWIYENCITGPLM
KNRTCILVTHNVSLTLRNAHFAIVLENGKVKNQGTITELQSKGLFKEKYVQLSSRDSI
NEKNANRLKAPRKNDSQKIEPVTENINFDANFVNDGQLIEEEEKSNGAISPDVYKWYL
KFFGGFKALTALFALYITAQILFISQSWWIRHWVNDTNVRINAPGFAMDTLPLKGMTD
SSKNKHNAFYYLTVYFLIGIIQAMLGGFKTMMTFLSGMRASRKIFNNLLDLVLHAQIR
FFDVTPVGRIMNRFSKDIEGVDQELIPYLEVTIFCLIQCASIIFLITVITPRFLTVAV
IVFVLYFFVGKWYLTASRELKRLDSITKSPIFQHFSETLVGVCTIRAFGDERRFILEN
MNKIDQNNRAFFYLSVTVKWFSFRVDMIGAFIVLASGSFILLNIANIDSGLAGISLTY
AILFTDGALWLVRLYSTFEMNMNSVERLKEYSSIEQENYLGHDEGRILLLNEPSWPKD
GEIEIENLSLRYAPNLPPVIRNVSFKVDPQSKIGIVGRTGAGKSTIITALFRLLEPIT
GCIKIDGQDISKIDLVTLRRSITIIPQDPILFAGTIKSNVDPYDEYDEKKIFKALSQV
NLISSHEFEEVLNSEERFNSTHNKFLNLHTEIAEGGLNLSQGERQLLFIARSLLREPK
IILLDEATSSIDYDSDHLIQGIIRSEFNKSTILTIAHRLRSVIDYDRIIVMDAGEVKE
YDRPSELLKDERGIFYSMCRDSGGLELLKQIAKQSSKMMK"
gene complement(<33193..>34077)
/gene="SBP1"
/locus_tag="YHL034C"
/gene_synonym="SSB1; SSBR1"
/db_xref="GeneID:856351"
mRNA complement(<33193..>34077)
/gene="SBP1"
/locus_tag="YHL034C"
/gene_synonym="SSB1; SSBR1"
/product="Sbp1p"
/transcript_id="NM_001179114.1"
/db_xref="GeneID:856351"
CDS complement(33193..34077)
/gene="SBP1"
/locus_tag="YHL034C"
/gene_synonym="SSB1; SSBR1"
/experiment="EXISTENCE:direct assay:GO:0000932 P-body
[PMID:23222640|PMID:16782896]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:2823109|PMID:2121740]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:16782896]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:23222640|PMID:26777405]"
/experiment="EXISTENCE:direct assay:GO:0017148 negative
regulation of translation [PMID:22284680]"
/experiment="EXISTENCE:direct assay:GO:0031370 eukaryotic
initiation factor 4G binding [PMID:22284680]"
/experiment="EXISTENCE:direct assay:GO:0035617 stress
granule disassembly [PMID:35440550]"
/experiment="EXISTENCE:direct assay:GO:0045947 negative
regulation of translational initiation
[PMID:39617253|PMID:28986506]"
/experiment="EXISTENCE:direct assay:GO:0048027 mRNA 5'-UTR
binding [PMID:28986506|PMID:39617253]"
/experiment="EXISTENCE:mutant phenotype:GO:0032055
negative regulation of translation in response to stress
[PMID:16782896]"
/experiment="EXISTENCE:mutant phenotype:GO:0045947
negative regulation of translational initiation
[PMID:28986506|PMID:39617253]"
/note="mRNA-binding translational repressor and P-body
disassembly factor; binds to the 5'UTR of mRNAs and
inhibits cap-dependent and independent translational
initiation; involved in mRNA binding under glucose
starvation stress; associates with snR10 and snR11;
HMT1-dependent arginine monomethylation modulates
translational repression, mRNA decay and stress granule
localization during glucose starvation; contains an
intrinsically disordered RGG motif; localizes to P bodies
and stress granules"
/codon_start=1
/product="Sbp1p"
/protein_id="NP_011829.1"
/db_xref="GeneID:856351"
/db_xref="SGD:S000001026"
/translation="MSAEIEEATNAVNNLSINDSEQQPRAPTHKTVIDPEDTIFIGNV
AHECTEDDLKQLFVEEFGDEVSVEIPIKEHTDGHIPASKHALVKFPTKIDFDNIKENY
DTKVVKDREIHIKRARTPGQMQRGGFRGRGGFRGRGGFRGGFRGGYRGGFRGRGNFRG
RGGARGGFNGQKREKIPLDQMERSKDTLYINNVPFKATKEEVAEFFGTDADSISLPMR
KMRDQHTGRIFTSDSANRGMAFVTFSGENVDIEAKAEEFKGKVFGDRELTVDVAVIRP
ENDEEEIEQETGSEEKQE"
gene complement(<35255..>36025)
/gene="RPL8A"
/locus_tag="YHL033C"
/gene_synonym="MAK7"
/db_xref="GeneID:856352"
mRNA complement(<35255..>36025)
/gene="RPL8A"
/locus_tag="YHL033C"
/gene_synonym="MAK7"
/product="60S ribosomal protein eL8 RPL8A"
/transcript_id="NM_001179113.1"
/db_xref="GeneID:856352"
CDS complement(35255..36025)
/gene="RPL8A"
/locus_tag="YHL033C"
/gene_synonym="MAK7"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:11983894]"
/experiment="EXISTENCE:curator inference:GO:0003735
structural constituent of ribosome [PMID:11983894]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:11983894]"
/experiment="EXISTENCE:mutant phenotype:GO:0000470
maturation of LSU-rRNA [PMID:22893726]"
/note="Ribosomal 60S subunit protein L8A; required for
processing of 27SA3 pre-rRNA to 27SB during assembly of
large subunit; binds Domain I of 25S, 5.8 S rRNAs;
mutation results in decreased amounts of free 60S
subunits; RPL8A has paralog RPL8B from whole genome
duplication; RPL8A or RPL8B ribosomes not functionally
interchangeable; proportions of Rpl8ap, Rpl8bp in 80S
ribosome differ in glucose and glycerol; homologous to
mammalian ribosomal protein L7A, no bacterial homolog"
/codon_start=1
/product="60S ribosomal protein eL8 RPL8A"
/protein_id="NP_011830.1"
/db_xref="GeneID:856352"
/db_xref="SGD:S000001025"
/translation="MAPGKKVAPAPFGAKSTKSNKTRNPLTHSTPKNFGIGQAVQPKR
NLSRYVKWPEYVRVQRQKKILSIRLKVPPTIAQFQYTLDRNTAAETFKLFNKYRPETA
AEKKERLTKEAAAVAEGKSKQDASPKPYAVKYGLNHVVALIENKKAKLVLIANDVDPI
ELVVFLPALCKKMGVPYAIVKGKARLGTLVNQKTSAVAALTEVRAEDEAALAKLVSTI
DANFADKYDEVKKHWGGGILGNKAQAKMDKRAKNSDSA"
gene complement(<36379..>38508)
/gene="GUT1"
/locus_tag="YHL032C"
/db_xref="GeneID:856353"
mRNA complement(<36379..>38508)
/gene="GUT1"
/locus_tag="YHL032C"
/product="glycerol kinase"
/transcript_id="NM_001179112.1"
/db_xref="GeneID:856353"
CDS complement(36379..38508)
/gene="GUT1"
/locus_tag="YHL032C"
/EC_number="2.7.1.30"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:17054397]"
/experiment="EXISTENCE:mutant phenotype:GO:0004370
glycerol kinase activity [PMID:8358828]"
/experiment="EXISTENCE:mutant phenotype:GO:0006071
glycerol metabolic process [PMID:8358828]"
/note="Glycerol kinase; converts glycerol to
glycerol-3-phosphate; glucose repression of expression is
mediated by Adr1p and Ino2p-Ino4p; derepression of
expression on non-fermentable carbon sources is mediated
by Opi1p and Rsf1p"
/codon_start=1
/product="glycerol kinase"
/protein_id="NP_011831.1"
/db_xref="GeneID:856353"
/db_xref="SGD:S000001024"
/translation="MFPSLFRLVVFSKRYIFRSSQRLYTSLKQEQSRMSKIMEDLRSD
YVPLIASIDVGTTSSRCILFNRWGQDVSKHQIEYSTSASKGKIGVSGLRRPSTAPARE
TPNAGDIKTSGKPIFSAEGYAIQETKFLKIEELDLDFHNEPTLKFPKPGWVECHPQKL
LVNVVQCLASSLLSLQTINSERVANGLPPYKVICMGIANMRETTILWSRRTGKPIVNY
GIVWNDTRTIKIVRDKWQNTSVDRQLQLRQKTGLPLLSTYFSCSKLRWFLDNEPLCTK
AYEENDLMFGTVDTWLIYQLTKQKAFVSDVTNASRTGFMNLSTLKYDNELLEFWGIDK
NLIHMPEIVSSSQYYGDFGIPDWIMEKLHDSPKTVLRDLVKRNLPIQGCLGDQSASMV
GQLAYKPGAAKCTYGTGCFLLYNTGTKKLISQHGALTTLAFWFPHLQEYGGQKPELSK
PHFALEGSVAVAGAVVQWLRDNLRLIDKSEDVGPIASTVPDSGGVVFVPAFSGLFAPY
WDPDARATIMGMSQFTTASHIARAAVEGVCFQARAILKAMSSDAFGEGSKDRDFLEEI
SDVTYEKSPLSVLAVDGGMSRSNEVMQIQADILGPCVKVRRSPTAECTALGAAIAANM
AFKDVNERPLWKDLHDVKKWVFYNGMEKNEQISPEAHPNLKIFRSESDDAERRKHWKY
WEVAVERSKGWLKDIEGEHEQVLENFQ"
gene complement(<38815..>39486)
/gene="GOS1"
/locus_tag="YHL031C"
/db_xref="GeneID:856354"
mRNA complement(<38815..>39486)
/gene="GOS1"
/locus_tag="YHL031C"
/product="Gos1p"
/transcript_id="NM_001179111.1"
/db_xref="GeneID:856354"
CDS complement(38815..39486)
/gene="GOS1"
/locus_tag="YHL031C"
/experiment="EXISTENCE:direct assay:GO:0005484 SNAP
receptor activity [PMID:11959998]"
/experiment="EXISTENCE:direct assay:GO:0005797 Golgi
medial cisterna [PMID:16699523]"
/experiment="EXISTENCE:direct assay:GO:0006906 vesicle
fusion [PMID:11959998]"
/experiment="EXISTENCE:direct assay:GO:0031201 SNARE
complex [PMID:11959998]"
/experiment="EXISTENCE:mutant phenotype:GO:0048193 Golgi
vesicle transport [PMID:9755865]"
/note="v-SNARE protein involved in Golgi transport;
homolog of the mammalian protein GOS-28/GS28"
/codon_start=1
/product="Gos1p"
/protein_id="NP_011832.1"
/db_xref="GeneID:856354"
/db_xref="SGD:S000001023"
/translation="MSSQPSFVTIRGKAISLETQTESLLSKYSTFAQTTSSEQTGQEK
KIDKQLEGILGQRQDVIDSLTQICDSNPAISASKLSQLHRHKEILQDHWKSFRNIRSS
IQQERNRLNLLFSVKNDIANSTTDAPAPIGDADEYIQNETRRIDQSNNVVDRLISQAW
ETRSQFHSQSNVLNTANNKVLQTLQRIPGVNQLIMKINTRRKKNAFVLATITTLCILF
LFFTW"
gene <40084..>45690
/gene="ECM29"
/locus_tag="YHL030W"
/db_xref="GeneID:856355"
mRNA <40084..>45690
/gene="ECM29"
/locus_tag="YHL030W"
/product="Ecm29p"
/transcript_id="NM_001179110.1"
/db_xref="GeneID:856355"
CDS 40084..45690
/gene="ECM29"
/locus_tag="YHL030W"
/experiment="EXISTENCE:direct assay:GO:0000502 proteasome
complex [PMID:12408819]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922|PMID:20620957|PMID:26928762|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0043248 proteasome
assembly [PMID:20620957]"
/experiment="EXISTENCE:direct assay:GO:0060090 molecular
adaptor activity [PMID:20620957]"
/experiment="EXISTENCE:mutant phenotype:GO:0042030 ATPase
inhibitor activity [PMID:23995839]"
/note="Scaffold protein; assists in association of
proteasome core particle with regulatory particle;
inhibitor of proteasome in vivo and in vitro; inhibits
proteasomal ATPase activity; degraded by the mature
proteasome after assembly; contains HEAT-like repeats;
protein increases in abundance and relocalizes from
nucleus to cytoplasm upon DNA replication stress"
/codon_start=1
/product="Ecm29p"
/protein_id="NP_011833.1"
/db_xref="GeneID:856355"
/db_xref="SGD:S000001022"
/translation="MSISSDEAKEKQLVEKAELRLAIADSPQKFETNLQTFLPPLLLK
LASPHASVRTAVFSALKNLISRINTLPQVQLPVRALIVQAKEPNLAAQQDSTNVRLYS
LLLASKGIDRLSLQDRQQLLPLVVSNISCLTGTVAARMFHILLKLILEWVAPQESSHE
QEEFVQFLQLDNDGFSFLMRQFTRFFLLVPSKQVQVSQQPLSRGYTCPGLSLTDVAFF
TYDAGVTFNKEQLNKFKKAIFQFVCRGMAATQTIEQSPRMIELMEFLCVVSTDSTNLS
DDAAQFMKRFPMPYENEEFITFLQTLYIGNTANGRPPVKAILQEKILSILNRSHFATT
KAECISLICSIGLHSSEYKLRSLTLSFIRHVAKLNYKNLNPASSSPSSTDFSTCIVSL
IRNNLHAEGWPKLQLGPQTPAFNTAILQRQLQYETLGDILKRDFELVSDLSYIEFLFE
SLKNDLPQFRSSIQESLLSLVGHLSILPQQSKLKLKNLLRKNLSIDEQQREDNNDAVN
SIMALKFVSIKFTNAAFPFHDPEARLFNIWGTVRTNRFDIIEESFKGLQPFWFRVNNA
SINTSATVKTSDLLGSHLSETEFPPFREFLQVLIDQLDSEAASITRKSLNNAVRFSKQ
CLISNAIYGKKTMVIQDEDWSVRIDKALELDDTVVSRVNEMVQGMNDDIFIRYLTLLS
NEFTATNSKGEQIAIFPYQDPIFGSVLLTLLNFVSNNVLRRLEILVPDLYHLVIMKFQ
SLSDNDLAVCATIIGIISTAIADSTHVKRITKIAQSQTMAETYVASYVVPRLYLKDQT
NHIESDSILNLLNILTTHLSHPGTNKDMILKLVCQVTKFGLLLQVSAQERKDFLKKVM
DTIQDKLINDVTAIQTWSYLSLYSTDLENSSLFQEKLLETNVSKQNDFLFSVGESLSV
VAGKWSSKYLIKQIDIPNFNVEIMQQKFPATNVTTILDEIFSGCDSTKPSLRKASCIW
LLSYIQYLGHLPEVSSKCNDIHLRFMRFLADRDEFIQDSAARGLSLVYEIGGSDLKES
MVKGLLKSFTESTAGSASTSATGISGSVSEETELFEPGVLNTGDGSISTYKDILNLAS
EVGDPALVYKFMSLAKSSALWSSRKGIAFGLGAIMSKSSLEELLLKDQQTAKKLIPKL
YRYRFDPFQAVSRSMTDIWNTLIPESSLTISLYFNDILDELLCGMANKEWRVREASTS
ALLQLIQSQPQEKFSEKMLKIWTMAFRTMDDIKDSVREVGTKFTTVLAKILARSIDVE
KGVNPTKSKEILDNILPFLWGPHGLNSDAEEVRNFALTTLIDLVKHSPGAIKPFTPKL
IYDFITLFSSIEPQVINYLALNAANYNIDANVIDTQRKNGVTNSPLFQTIEKLINNSD
DCMMEEIINVVIKASRKSVGLPSKVASSLVIIILVKRYSIEMKPYSGKLLKVCLTMFE
DRNESVNIAFAISMGYLFKVSALDKCIKYSEKLITKYFEPTSTENNKKVVGTAIDSIL
NYAKSEFDNVASVFMPLIFIACNDEDKDLETLYNKIWTEASSSGAGTVKLYLPEILNV
LCVNIKSNDFSIRKTCAKSVIQLCGGINDSIPYPQIVKLFDISREALSGRSWDGKEHI
VAALVSLTEKFSQTVADNNDLQESINHVMYTEVSRKSMKYVKKILPLYARYINVNPQE
ETITFLIEKAKEMIRLLGSESDDSEGSIKQTSDESTIKRIKPNTEITQKSSKENIENE
EYVINLLKVSVDICNNSKSRYPMNLLEFIIDEIAYLFHNDRIIHTWRTQLAASEIGIS
IVGRFSTISSADFIQNVGRLWDQTFPINCNKETIENVKLQMIKFGGLIIQKIPSLQNN
IEENLRLLNSIDSTSRIELELKNIGL"
gene complement(<45929..>47968)
/gene="OCA5"
/locus_tag="YHL029C"
/db_xref="GeneID:856356"
mRNA complement(<45929..>47968)
/gene="OCA5"
/locus_tag="YHL029C"
/product="Oca5p"
/transcript_id="NM_001179109.1"
/db_xref="GeneID:856356"
CDS complement(45929..47968)
/gene="OCA5"
/locus_tag="YHL029C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0052845
inositol-5-diphosphate-1,2,3,4,6-pentakisphosphate
diphosphatase activity [PMID:36758548]"
/experiment="EXISTENCE:mutant phenotype:GO:0071543
diphosphoinositol polyphosphate metabolic process
[PMID:36758548]"
/note="Inositol pyrophosphatase that catalyzes the
conversion of 5-InsP7 to InsP6; controls the balance
between glycolysis and respiration; localizes to the
cytoplasm; required for replication of Brome mosaic virus;
S. cerevisiae is a model system for studying replication
of positive-strand RNA viruses in their natural host"
/codon_start=1
/product="Oca5p"
/protein_id="NP_011834.1"
/db_xref="GeneID:856356"
/db_xref="SGD:S000001021"
/translation="MHDKKSPMANSHYLKNLKQQFRNKNLIETTIHLVKCNDHDSLAF
LARTYGVPPQLRHVVWPILLKYHPMCISPNITSNTISWDPITNDFILNDPFLKSKAPT
DKQDKSDDENILPYDIESIILHDLKKYFHSRSNPAGSSSNANTTNIATPTPVSSSDAS
TISSMEVLSPSLDYEFQIIETLKNAIVKFLLKWSKIFKYESGLAWIALGLAEWYPIYP
YETMSPFNETHSFYEVEDYVVLSGRKHALLSTNNGNNGNSNSSSNNTNNNNTNITSGM
HNLSINTNTSLHNSPYISHTLSYLYKEYPLPFELRSKLPTKPIFSFSALFERLALVIL
HCPDTILAHKQLKNDSNASSSSKANSNFNTNYFPIISGGDLSFQTQVFFKVFSSILPE
LYQPLTEESSLQPSSSRNSWIYWWLKCSGAKALQRQDRGRVWDLLLGWRPKPNMDTIN
FFLNYNDKKMDHLYHDTPQCDNEQYWMKDWIALYNNDPFWFPDLDSMALGSKKFPYDY
SVFKELILRNRYGGTQSKAQKDNTVPSPGSDSNDKSELKLPFSSIDPHMQLIFIFIAI
LQFNEFKLLEFEEAEISEFLNNVPLLTKFDDSSYRKLYENTESSITSLPSSPTTSTMA
SLQSSSNSSAHISNYHMLIEVGNDAKASHCFDDLLNMAGDIWRKWLWRELEESSL"
gene <48763..>50580
/gene="WSC4"
/locus_tag="YHL028W"
/gene_synonym="YFW1; YHC8"
/db_xref="GeneID:856357"
mRNA <48763..>50580
/gene="WSC4"
/locus_tag="YHL028W"
/gene_synonym="YFW1; YHC8"
/product="Wsc4p"
/transcript_id="NM_001179108.1"
/db_xref="GeneID:856357"
CDS 48763..50580
/gene="WSC4"
/locus_tag="YHL028W"
/gene_synonym="YFW1; YHC8"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:10196219]"
/experiment="EXISTENCE:genetic interaction:GO:0009408
response to heat [PMID:11589572]"
/experiment="EXISTENCE:mutant phenotype:GO:0006612 protein
targeting to membrane [PMID:10196219]"
/experiment="EXISTENCE:mutant phenotype:GO:0008320 protein
transmembrane transporter activity [PMID:10196219]"
/experiment="EXISTENCE:mutant phenotype:GO:0045047 protein
targeting to ER [PMID:10196219]"
/note="Endoplasmic reticulum (ER) membrane protein;
involved in the translocation of soluble secretory
proteins and insertion of membrane proteins into the ER
membrane; may also have a role in the stress response but
has only partial functional overlap with WSC1-3"
/codon_start=1
/product="Wsc4p"
/protein_id="NP_011835.1"
/db_xref="GeneID:856357"
/db_xref="SGD:S000001020"
/translation="MQTSMVSAKVSIWLVCSVICSSLVRATQSVCSSQNTATTDGVRN
QFQSNGWCSNNCAGHQFAIVQGFMCWCSDSEPSTQTSVGDCSGTCPGYGYEDCGNADK
DLFGYIYLGQTPLSSVQSVETSTESSVYVSSSSITSSSSTSIVDTTTISPTLTSTSTT
PLTTASTSTTPSTDITSALPTTTSTKLSTSIPTSTTSSTSTTTSTSSSTSTTVSVTSS
TSTTTSTTSSTLISTSTSSSSSSTPTTTSSAPISTSTTSSTSTSTSTTSPTSSSAPTS
SSNTTPTSTTFTTTSPSTAPSSTTVTYTSTTASPITSTITSVNLQTSLKYSVITVTSV
HTMDTNISEITSRYLTMKKVITQIYSSTLGATPTSAVATTSASVGGRITNNNNSNTTN
SNTPTNKSTEKKGYWDSPGKIAATFVVVGVVCLVIICILIYLIHHYRTRPARKAQDFE
NEYQSKFYQSKYPNEVTTTTLHTPSPSSNSTFSTPRLIYTDEKGQIMSESPSPRQSTY
SLTAGSPPNDPSTLASPFHDPILPRRTSTFLHSPIQKQHEKMESNVTLGEDTVLVDQR
LDPSKMLNTLANDDATNHSTISLSDNVDYSRRVLRLMNE"
gene <51111..>52988
/gene="RIM101"
/locus_tag="YHL027W"
/gene_synonym="RIM1"
/db_xref="GeneID:856358"
mRNA <51111..>52988
/gene="RIM101"
/locus_tag="YHL027W"
/gene_synonym="RIM1"
/product="alkaline-responsive transcriptional regulator
RIM101"
/transcript_id="NM_001179107.1"
/db_xref="GeneID:856358"
CDS 51111..52988
/gene="RIM101"
/locus_tag="YHL027W"
/gene_synonym="RIM1"
/experiment="EXISTENCE:direct assay:GO:0000122 negative
regulation of transcription by RNA polymerase II
[PMID:12509465]"
/experiment="EXISTENCE:direct assay:GO:0001227 DNA-binding
transcription repressor activity, RNA polymerase
II-specific [PMID:16024810]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:20382759]"
/experiment="EXISTENCE:genetic interaction:GO:0001227
DNA-binding transcription repressor activity, RNA
polymerase II-specific [PMID:16024810]"
/experiment="EXISTENCE:genetic interaction:GO:0009272
fungal-type cell wall biogenesis [PMID:16524906]"
/experiment="EXISTENCE:genetic interaction:GO:0010973
positive regulation of division septum assembly
[PMID:19633265]"
/experiment="EXISTENCE:mutant phenotype:GO:0001227
DNA-binding transcription repressor activity, RNA
polymerase II-specific [PMID:16024810]"
/experiment="EXISTENCE:mutant phenotype:GO:0009272
fungal-type cell wall biogenesis [PMID:16524906]"
/experiment="EXISTENCE:mutant phenotype:GO:0030437
ascospore formation [PMID:8417990]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:8417990]"
/experiment="EXISTENCE:mutant phenotype:GO:0051321 meiotic
cell cycle [PMID:8367297]"
/experiment="EXISTENCE:mutant phenotype:GO:0071454
cellular response to anoxia [PMID:20402793]"
/experiment="EXISTENCE:mutant phenotype:GO:0071469
cellular response to alkaline pH [PMID:11050096]"
/note="Cys2His2 zinc-finger transcriptional repressor;
involved in alkaline responsive gene repression; involved
in cell wall assembly; plays role in lager yeast
flocculation under brewing conditions; required for
alkaline pH-stimulated haploid invasive growth and
sporulation; activated by alkaline-dependent proteolytic
processing which results in removal of the C-terminal
tail; similar to A. nidulans PacC"
/codon_start=1
/product="alkaline-responsive transcriptional regulator
RIM101"
/protein_id="NP_011836.1"
/db_xref="GeneID:856358"
/db_xref="SGD:S000001019"
/translation="MVPLEDLLNKENGTAAPQHSRESIVENGTDVSNVTKKDGLPSPN
LSKRSSDCSKRPRIRCTTEAIGLNGQEDERMSPGSTSSSCLPYHSTSHLNTPPYDLLG
ASAVSPTTSSSSDSSSSSPLAQAHNPAGDDDDADNDGDSEDITLYCKWDNCGMIFNQP
ELLYNHLCHDHVGRKSHKNLQLNCHWGDCTTKTEKRDHITSHLRVHVPLKPFGCSTCS
KKFKRPQDLKKHLKIHLESGGILKRKRGPKWGSKRTSKKNKSCASDAVSSCSASVPSA
IAGSFKSHSTSPQILPPLPVGISQHLPSQQQQRAISLNQLCSDELSQYKPVYSPQLSA
RLQTILPPLYYNNGSTVSQGANSRSMNVYEDGCSNKTIANATQFFTKLSRNMTNNYIL
QQSGGSTESSSSSGRIPVAQTSYVQPPNAPSYQSVQGGSSISATANTATYVPVRLAKY
PTGPSLTEHLPPLHSNTAGGVFNRQSQYAMPHYPSVRAAPSYSSSGCSILPPLQSKIP
MLPSRRTMAGGTSLKPNWEFSLNQKSCTNDIIMSKLAIEEVDDESEIEDDFVEMLGIV
NIIKDYLLCCVMEDLDDEESEDKDEENAFLQESLEKLSLQNQMGTNSVRILTKYPKIL
V"
gene complement(<53219..>54166)
/locus_tag="YHL026C"
/db_xref="GeneID:856359"
mRNA complement(<53219..>54166)
/locus_tag="YHL026C"
/product="uncharacterized protein"
/transcript_id="NM_001179106.1"
/db_xref="GeneID:856359"
CDS complement(53219..54166)
/locus_tag="YHL026C"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="hypothetical protein; transcriptionally regulated
by Upc2p via an upstream sterol response element; SWAT-GFP
fusion protein localizes to the cell periphery, while
mCherry fusion localizes to both the cell periphery and
vacuole; YHL026C is not an essential gene; in 2005 the
start site was moved 141 nt upstream (see Locus History)"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011837.2"
/db_xref="GeneID:856359"
/db_xref="SGD:S000001018"
/translation="MKSSEPAPATPTGFRNSIWFIIFYLFVIQALGSAIISGGIEFAI
AYAMYHSRVDLITLWAFPHTISGDCALSLFIQVGLTWASEEILVGFDDYKRPVFRLNK
WITKPSPLKTESNEEIPPPKKRFIVDYFESKDNVVAKQNTLYHKHNWLFGYLEVNRGI
IPKGKEATLKGFLTSQFIHDSTQSKFMNFIEWFVQKFIRSMILAIAMFIVIWPVTMGI
LAGIGHKVGSHDYYFNDYPLPQVMKLIYAVVIAFVCTPVAIIVIVLRNQFHEELYYEG
LANGTLQQDQEVCSTGNRSSGSTDQDISTTKQQSQEAVA"
gene <54851..>55849
/gene="SNF6"
/locus_tag="YHL025W"
/db_xref="GeneID:856360"
mRNA <54851..>55849
/gene="SNF6"
/locus_tag="YHL025W"
/product="Snf6p"
/transcript_id="NM_001179105.1"
/db_xref="GeneID:856360"
CDS 54851..55849
/gene="SNF6"
/locus_tag="YHL025W"
/experiment="EXISTENCE:direct assay:GO:0000182 rDNA
binding [PMID:23437238]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:2188093|PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0016514 SWI/SNF
complex
[PMID:8159677|PMID:8127913|PMID:18644858|PMID:8016655]"
/experiment="EXISTENCE:genetic interaction:GO:0006338
chromatin remodeling [PMID:1459453]"
/experiment="EXISTENCE:genetic interaction:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:1901413]"
/experiment="EXISTENCE:mutant phenotype:GO:0000470
maturation of LSU-rRNA [PMID:23437238]"
/experiment="EXISTENCE:mutant phenotype:GO:0006289
nucleotide-excision repair [PMID:17013386]"
/experiment="EXISTENCE:mutant phenotype:GO:0006338
chromatin remodeling [PMID:17013386]"
/experiment="EXISTENCE:mutant phenotype:GO:0016514 SWI/SNF
complex [PMID:8159677]"
/experiment="EXISTENCE:mutant phenotype:GO:0045943
positive regulation of transcription by RNA polymerase I
[PMID:23437238]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:1339306]"
/experiment="EXISTENCE:physical interaction:GO:0006289
nucleotide-excision repair [PMID:17013386]"
/note="Subunit of the SWI/SNF chromatin remodeling
complex; involved in transcriptional regulation; functions
interdependently in transcriptional activation with Snf2p
and Snf5p; relocates to the cytosol under hypoxic
conditions"
/codon_start=1
/product="Snf6p"
/protein_id="NP_011838.1"
/db_xref="GeneID:856360"
/db_xref="SGD:S000001017"
/translation="MGVIKKKRSHHGKASRQQYYSGVQVGGVGSMGAINNNIPSLTSF
AEENNYQYGYSGSSAGMNGRSLTYAQQQLNKQRQDFERVRLRPEQLSNIIHDESDTIS
FRSNLLKNFISSNDAFNMLSLTTVPCDRIEKSRLFSEKTIRYLMQKQHEMKTQAAELQ
EKPLTPLKYTKLIAAAEDGSRSTKDMIDAVFEQDSHLRYQPDGVVVHRDDPALVGKLR
GDLREAPADYWTHAYRDVLAQYHEAKERIRQKEVTAGEAQDEASLQQQQQQDLQQQQQ
VVTTVASQSPHATATEKEPVPAVVDDPLENMFGDYSNEPFNTNFDDEFGDLDAVFF"
gene <56649..>58790
/gene="RIM4"
/locus_tag="YHL024W"
/db_xref="GeneID:856361"
mRNA <56649..>58790
/gene="RIM4"
/locus_tag="YHL024W"
/product="Rim4p"
/transcript_id="NM_001179104.1"
/db_xref="GeneID:856361"
CDS 56649..58790
/gene="RIM4"
/locus_tag="YHL024W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:40593583]"
/experiment="EXISTENCE:direct assay:GO:0017148 negative
regulation of translation [PMID:37921330]"
/experiment="EXISTENCE:direct assay:GO:0042149 cellular
response to glucose starvation [PMID:37921330]"
/experiment="EXISTENCE:direct assay:GO:0048027 mRNA 5'-UTR
binding [PMID:37921330]"
/experiment="EXISTENCE:mutant phenotype:GO:0003723 RNA
binding [PMID:10806425]"
/experiment="EXISTENCE:mutant phenotype:GO:0006279
premeiotic DNA replication [PMID:11713679]"
/experiment="EXISTENCE:mutant phenotype:GO:0007131
reciprocal meiotic recombination [PMID:11713679]"
/experiment="EXISTENCE:mutant phenotype:GO:0017148
negative regulation of translation [PMID:37921330]"
/experiment="EXISTENCE:mutant phenotype:GO:0030435
sporulation resulting in formation of a cellular spore
[PMID:10806425]"
/experiment="EXISTENCE:mutant phenotype:GO:0042149
cellular response to glucose starvation [PMID:37921330]"
/experiment="EXISTENCE:mutant phenotype:GO:0048027 mRNA
5'-UTR binding [PMID:37921330]"
/experiment="EXISTENCE:mutant phenotype:GO:0051321 meiotic
cell cycle [PMID:11713679]"
/note="RNA-binding protein; 5'UTR binding protein that
forms amyloid-like translation-repressing aggregates in
meiosis, induced by starvation conditions; involved in
regulation of early and middle sporulation genes; contains
three RNA recognition motifs (RRM1-3) and a C-terminal
intrinsically-disordered region containing a prion-like
domain involved in the conversion of the monomeric form
into amyloid-like aggregates; PKA signaling inhibits Rim4p
assembly and promotes assembly clearance via the IDR"
/codon_start=1
/product="Rim4p"
/protein_id="NP_011839.1"
/db_xref="GeneID:856361"
/db_xref="SGD:S000001016"
/translation="MKTEISTADSLRDPPSNGLKADSELVIREDIDQFLPSEVSSLGS
DHQNDGEDSDTDSDNFLQDPEDDVDEESTGRGTVTTTSTSTESRGRPSSCIFVASLAA
ALSDDELCLSVTENFKKYGDLARVKVLRDNANRPYAFVQYNNDHDAKHALIRAQGTLL
NGRRLRCEPAKVNRTLYLKNQQSIDFNEISQICEKFGGLEQIVPDRTDNQYTRRYTYP
ISSANSWFVQFVYRDDAIRAYANLRTDPNWIIEWAQNINVPKNYNLLHKSKFKSSKYH
QNNGIINNDGSNNNDNNNSNNNNREDSRRNGDVIEEECGHVHGSDSEEKLTSDGIYDD
EDKDSEITIDKRSIFVGQLDKETTREELNRRFSTHGKIQDINLIFKPTNIFAFIKYET
EEAAAAALESENHAIFLNKTMHVQYKEVGGRHNRKFSGKNGGSNFNHHQFFSTRSGKT
FTGPELNLAPPPINMYRKMSGGSQQESETMMPYMPMGPMPMGPPPPNAASLSDFDMFP
PSYSTFMKGMMPLRRKSMPNSWSSPSSKSVNSENESVNGGDENSELPSEIPESSGRYN
AANSFTTYNNSSAGNSNNNNNNNNSNSNKSQYKKRYARRSSYGYNEVPPKPYYFQPYY
YHPMQYHMGPMGPLHPSQGSAGNHHPYMMVYPMSPPPPSGLDGSMIPPPINVSQSHAA
NHGSTHVHANEFISNDTGDINEDNKAYSLDY"
gene complement(<59123..>62563)
/gene="NPR3"
/locus_tag="YHL023C"
/gene_synonym="RMD11"
/db_xref="GeneID:856362"
mRNA complement(<59123..>62563)
/gene="NPR3"
/locus_tag="YHL023C"
/gene_synonym="RMD11"
/product="Npr3p"
/transcript_id="NM_001179103.1"
/db_xref="GeneID:856362"
CDS complement(59123..62563)
/gene="NPR3"
/locus_tag="YHL023C"
/gene_synonym="RMD11"
/experiment="EXISTENCE:direct assay:GO:0035859
Seh1-associated complex [PMID:21454883]"
/experiment="EXISTENCE:direct assay:GO:1990130 GATOR1
complex [PMID:21900499]"
/experiment="EXISTENCE:genetic interaction:GO:0006995
cellular response to nitrogen starvation [PMID:25046117]"
/experiment="EXISTENCE:genetic interaction:GO:0010508
positive regulation of autophagy [PMID:25046117]"
/experiment="EXISTENCE:genetic interaction:GO:0051058
negative regulation of small GTPase mediated signal
transduction [PMID:23716719]"
/experiment="EXISTENCE:genetic interaction:GO:1904262
negative regulation of TORC1 signaling
[PMID:25046117|PMID:23716719]"
/experiment="EXISTENCE:mutant phenotype:GO:0006995
cellular response to nitrogen starvation [PMID:25046117]"
/experiment="EXISTENCE:mutant phenotype:GO:0007124
pseudohyphal growth [PMID:24603354]"
/experiment="EXISTENCE:mutant phenotype:GO:0010508
positive regulation of autophagy [PMID:25046117]"
/experiment="EXISTENCE:mutant phenotype:GO:0034198
cellular response to amino acid starvation
[PMID:19521502]"
/experiment="EXISTENCE:mutant phenotype:GO:0051321 meiotic
cell cycle [PMID:18069899]"
/experiment="EXISTENCE:mutant phenotype:GO:1904262
negative regulation of TORC1 signaling
[PMID:23716719|PMID:19521502|PMID:25046117]"
/experiment="EXISTENCE:mutant phenotype:GO:2000785
regulation of autophagosome assembly [PMID:21900499]"
/note="Subunit of the Iml1p/SEACIT complex; SEACIT
(Iml1p-Npr2p-Npr3p) is a subcomplex of SEAC, a
coatomer-related complex that associates dynamically with
the vacuole; Npr3p may have a structural or regulatory
role, supporting Iml1p function as a GAP for the Rag
family GTPase Gtr1p, and leading to inhibition of TORC1
signaling in response to amino acid deprivation; SEACIT is
required for non-nitrogen-starvation-induced autophagy;
null mutant has meiotic defects; human NPRL3 homolog"
/codon_start=1
/product="Npr3p"
/protein_id="NP_011840.1"
/db_xref="GeneID:856362"
/db_xref="SGD:S000001015"
/translation="MDECLPNSCLLGVHLVISTHSGPQIVYHYPPSNTAFLTNNPTKH
QHLYGNHANLNKNTSTNKEEKLFNSGSTKTASQIALNESAKSYNTAITPSMTNTNTNN
VTLPPTRSHANTVGSQSSIPAATNGVGYRKTDIEDTSRTFQYQETESETSSSGLSDSE
LSTDYLDISSDSFSISSSLSSSSLSSSPSSSSSSSPPQDGLSRTNSSFQSTDSMSPTS
PQMIMENDSISVAESYLDSGTNNKSRAASKRSQNFFHKLSTKKSTDSKTHSPVRKLKS
KPSQSTKKGNKLLKNTSNETDGNAFTGSCSISSKKSLSSTGEHNQELRNSSLNDTPGQ
SPHHYHHRYHHYHKNAATSQRNSHTQYDVEEEDMEVSAMLQDGKISMNEIFFEEENFQ
DINKILEFDNDFVAEFCSPEREMCNTRFEFTVDNFCFLGLPIHVDSQGRWRKSKHKNK
TRSKRSSSTTTNISRKKSIASKISSLSENTLKKVNSGEADTVYDSNIGHEASTDTPNL
RINTDVSGNEFEREKEDLGKNMNMFHVCFVMNPHLIEYNKRIDDMYQFVVTRLSLLLR
YVQSKTSYISSECHIILKEKERVLKHSKTYQSIRGAGNKGKYLYQRILAKSSLARALT
ECVDKIQRNEIACLEINDDKVISLQIPIQNEFEKMPNFKLQPVLRGSYLTSILNMKFL
EKSSLRIESQNRQNDQAQFSDTNNNIYRFGNNINSTGHCGAANVDDGDDNESNYYCDD
NDDLLNYALLLLDEPNNIISSLETFSYQDDIGTIILKHLVRNIQPNIPLRSYRYLITD
LLDNPSSLDDLTTETNSLESSILRSCALHLMYWRHARIVIPLSSKYTYIVSPLAPIQG
YTIDDYKSTSQNDGNVKKMDDRENNKSGSDRVPLIYQNSMLFRSKFPSLPSLPIFLSL
LSTDKPQAYSNIIPSREHKPVYLNALAWLIQYGYVTQLLTFINIRVDKHIKMAVDEDL
EKEGFRKTNTARRPSMDYKKTDKKLDDEDGQSRDANASEACSGKNEGMQSNDNNKDVD
EKDNENDSRVDDRDDNEIAIADEEEILHFEYDDPEMQHDYTIILEPERATAIEKRWLY
RCIYGQPSDIQILFNKLLKYFNGKVPMELVIIKEEISRHDLKKLLNALDKYLIEIHHW
"
gene 62755..62826
/locus_tag="YNCH0001W"
/db_xref="GeneID:856363"
tRNA 62755..62826
/locus_tag="YNCH0001W"
/product="tRNA-His"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0030371 translation
repressor activity [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043555 regulation
of translation in response to stress [PMID:27609601]"
/note="Histidine tRNA (tRNA-His), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:856363"
/db_xref="SGD:S000006597"
gene complement(<62961..>64157)
/gene="SPO11"
/locus_tag="YHL022C"
/db_xref="GeneID:856364"
mRNA complement(<62961..>64157)
/gene="SPO11"
/locus_tag="YHL022C"
/product="DNA topoisomerase (ATP-hydrolyzing)"
/transcript_id="NM_001179102.1"
/db_xref="GeneID:856364"
CDS complement(62961..64157)
/gene="SPO11"
/locus_tag="YHL022C"
/EC_number="5.6.2.2"
/experiment="EXISTENCE:direct assay:GO:0000794 condensed
nuclear chromosome [PMID:9039264]"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:23246437]"
/experiment="EXISTENCE:direct assay:GO:0007127 meiosis I
[PMID:9039264]"
/experiment="EXISTENCE:direct assay:GO:0035861 site of
double-strand break [PMID:23246437]"
/experiment="EXISTENCE:direct assay:GO:0045027 DNA end
binding [PMID:9039264]"
/experiment="EXISTENCE:genetic interaction:GO:0042138
meiotic DNA double-strand break formation [PMID:2190690]"
/experiment="EXISTENCE:mutant phenotype:GO:0007129
homologous chromosome pairing at meiosis [PMID:8207053]"
/experiment="EXISTENCE:mutant phenotype:GO:0007130
synaptonemal complex assembly [PMID:2687110]"
/experiment="EXISTENCE:mutant phenotype:GO:0042138 meiotic
DNA double-strand break formation [PMID:2190690]"
/note="Meiosis-specific protein required for meiotic
recombination; makes DNA double-strand breaks (DSB) during
meiotic recombination, homolog pairing, and synaptonemal
complex formation; role in meiotic DSB formation involves
a topo-II-like transesterase mechanism; forms
monotetrameric complex; covalently linked to DSBs through
a 5'-phosphodiester bond between a catalytic tyrosine
(Y135) and the DSB 5'- end; sequence similarity to a topo
VI subunit from archaea, fission yeast rec12 and human
SPO11"
/codon_start=1
/product="DNA topoisomerase (ATP-hydrolyzing)"
/protein_id="NP_011841.1"
/db_xref="GeneID:856364"
/db_xref="SGD:S000001014"
/translation="MALEGLRKKYKTRQELVKALTPKRRSIHLNSNGHSNGTPCSNAD
VLAHIKHFLSLAANSLEQHQQPISIVFQNKKKKGDTSSPDIHTTLDFPLNGPHLCTHQ
FKLKRCAILLNLLKVVMEKLPLGKNTTVRDIFYSNVELFQRQANVVQWLDVIRFNFKL
SPRKSLNIIPAQKGLVYSPFPIDIYDNILTCENEPKMQKQTIFPGKPCLIPFFQDDAV
IKLGTTSMCNIVIVEKEAVFTKLVNNYHKLSTNTMLITGKGFPDFLTRLFLKKLEQYC
SKLISDCSIFTDADPYGISIALNYTHSNERNAYICTMANYKGIRITQVLAQNNEVHNK
SIQLLSLNQRDYSLAKNLIASLTANSWDIATSPLKNVIIECQREIFFQKKAEMNEIDA
RIFEYK"
rep_origin 64296..64371
/note="ARS805; Autonomously Replicating Sequence"
/db_xref="SGD:S000114849"
gene complement(<64462..>65859)
/gene="AIM17"
/locus_tag="YHL021C"
/gene_synonym="FMP12"
/db_xref="GeneID:856365"
mRNA complement(<64462..>65859)
/gene="AIM17"
/locus_tag="YHL021C"
/gene_synonym="FMP12"
/product="Aim17p"
/transcript_id="NM_001179101.1"
/db_xref="GeneID:856365"
CDS complement(64462..65859)
/gene="AIM17"
/locus_tag="YHL021C"
/gene_synonym="FMP12"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:14576278|PMID:14562095|PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:mutant phenotype:GO:0007005
mitochondrion organization [PMID:19300474]"
/note="hypothetical protein; the authentic, non-tagged
protein is detected in highly purified mitochondria in
high-throughput studies; null mutant displays reduced
frequency of mitochondrial genome loss"
/codon_start=1
/product="Aim17p"
/protein_id="NP_011842.1"
/db_xref="GeneID:856365"
/db_xref="SGD:S000001013"
/translation="MLRSNLCRGSRILARLTTTPRTYTSAATAAAANRGHIIKTYFNR
DSTTITFSMEESSKPVSVCFNNVFLRDASHSAKLVTTGELYHNEKLTAPQDIQISEDG
KSLVVKWKDGGHHQFPLQFFIDYKGSSFVSPATRKQESRYRPQLWNKRILKDNVKDLL
SVSYNEFIDPKDDSKLFQTLVNLQKFGIAFISGTPSSSSEGLTIQKICERIGPIRSTV
HGEGTFDVNASQATSVNAHYANKDLPLHTDLPFLENVPGFQILQSLPATEGEDPNTRP
MNYFVDAFYATRNVRESDFEAYEALQIVPVNYIYENGDKRYYQSKPLIEHHDINEDNT
LLGNYEALIKCINYSPPYQAPFTFGIYDKPSDLNNNLDLNLITTPAKLTERFLFKSFI
RGLNLFESHINDFNNQFRLQLPENCCVIFNNRRILHANSLTSSNQQWLKGCYFDSDTF
KSKLKFLEEKFPHDK"
gene complement(<66242..>67456)
/gene="OPI1"
/locus_tag="YHL020C"
/db_xref="GeneID:856366"
mRNA complement(<66242..>67456)
/gene="OPI1"
/locus_tag="YHL020C"
/product="transcriptional regulator OPI1"
/transcript_id="NM_001179100.1"
/db_xref="GeneID:856366"
CDS complement(66242..67456)
/gene="OPI1"
/locus_tag="YHL020C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:27663510|PMID:15192221|PMID:15455074]"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:15192221]"
/experiment="EXISTENCE:direct assay:GO:0005654 nucleoplasm
[PMID:15455074]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:15192221]"
/experiment="EXISTENCE:direct assay:GO:0016036 cellular
response to phosphate starvation [PMID:27743455]"
/experiment="EXISTENCE:direct assay:GO:0031965 nuclear
membrane [PMID:15455074]"
/experiment="EXISTENCE:direct assay:GO:0034399 nuclear
periphery [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0070300
phosphatidic acid binding [PMID:27480805]"
/experiment="EXISTENCE:direct assay:GO:0071072 negative
regulation of phospholipid biosynthetic process
[PMID:27743455]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:1985968]"
/experiment="EXISTENCE:mutant phenotype:GO:0008654
phospholipid biosynthetic process [PMID:1985968]"
/experiment="EXISTENCE:mutant phenotype:GO:0030968
endoplasmic reticulum unfolded protein response
[PMID:16582425]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:12871953]"
/experiment="EXISTENCE:physical interaction:GO:0003714
transcription corepressor activity [PMID:11454208]"
/note="Transcriptional regulator of a variety of genes;
critical sensor of genotoxic stress; phosphorylation by
protein kinase A stimulates Opi1p function in negative
regulation of phospholipid biosynthetic genes; involved in
telomere maintenance; null exhibits disrupted
mitochondrial metabolism and low cardiolipin content,
strongly correlated with overproduction of inositol; binds
to phosphatidic acid"
/codon_start=1
/product="transcriptional regulator OPI1"
/protein_id="NP_011843.1"
/db_xref="GeneID:856366"
/db_xref="SGD:S000001012"
/translation="MSENQRLGLSEEEVEAAEVLGVLKQSCRQKSQPSEDVSQADKMP
ASESSTTPLNILDRVSNKIISNVVTFYDEINTNKRPLKSIGRLLDDDDDEHDDYDYND
DEFFTNKRQKLSRAIAKGKDNLKEYKLNMSIESKKRLVTCLHLLKLANKQLSDKISCL
QDLVEKEQVHPLHKQDGNARTTTGAGEDETSSDEDDDDEEFFDASEQVNASEQSIVVK
MEVVGTVKKVYSLISKFTANSLPEPARSQVRESLLNLPTNWFDSVHSTSLPHHASFHY
ANCEEQKVEQQQQQQQQQQQQQLLQQQLLQQQQQKRNKDGDDSASPSSSVTANGKVLI
LAKESLEMVRNVMGVVDSTLGKAEEWVKQKQEVKEMIRERFLQQQQQYRQQQQKDGNY
VKPSQDNVDSKD"
gene complement(<67731..>69548)
/gene="APM2"
/locus_tag="YHL019C"
/db_xref="GeneID:856367"
mRNA complement(<67731..>69548)
/gene="APM2"
/locus_tag="YHL019C"
/product="Apm2p"
/transcript_id="NM_001179099.1"
/db_xref="GeneID:856367"
CDS complement(67731..69548)
/gene="APM2"
/locus_tag="YHL019C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005769 early
endosome [PMID:26658609]"
/experiment="EXISTENCE:direct assay:GO:0005802 trans-Golgi
network [PMID:26658609]"
/experiment="EXISTENCE:mutant phenotype:GO:0006896 Golgi
to vacuole transport [PMID:17003107]"
/note="One of two alternative Mu1-like medium subunits of
the clathrin-associated AP-1 complex; binds clathrin;
involved in clathrin-dependent Golgi protein sorting;
homologous to the medium chain of mammalian
clathrin-associated protein complex"
/codon_start=1
/product="Apm2p"
/protein_id="NP_011844.1"
/db_xref="GeneID:856367"
/db_xref="SGD:S000001011"
/translation="MSSSLFILDENLEPLVSKNIRALPNLSSVLSSFKQCYHDGSPPI
LSQNDWFFIHLKRDFLHFVSVIHTTDKPNIDLMTILAFLEQFYHLLQKYFEIEVLTKN
VILDNILLVLELIDECIDFGIVQVTDPSIIKDYIRVKVNVPRVTVDNEEWSPGEESSS
SSGSDSDSEYSNTNKRKDKKKKRKKKKGTKGKSVGKSKLKSIMVNNKENRGINVVETV
KETLRNKNDTGKEAANDELPNDGNDLYINGDIAKTIIMPISWRTKGIHYAKNEFFLDV
IERVQYLMDFEKGVIRKNLIHGEIVCRCYLSGMPKLKISINKILNRDPQFMSNSSFHQ
CVSLDSINTIEKDEEKNSDDDAGLQAATDAREIEFIPPDGEFVLCQYELKRHVKDAPM
VRLKDFEIKPKLKKFKIQIVTKIQTNFKPTNSTSKLNVRIPLTKVFQEYKIDLSKQIR
FKANIGKVVFNLSDDFLLWEIQTMKGHREHSTNKSSQYNSDEDDPNTCASMVAEFPLF
NQEEYDRLQEEMKTSMNPPPLRTGPRLEELYRQVHDQQTSHVTPRDKLVNIDFEIPYC
TCSGLKVEYLKVEEPQLQYQSFPWVRYKTVSDEEYAYIV"
gene <69708..>70070
/gene="MCO14"
/locus_tag="YHL018W"
/db_xref="GeneID:856368"
mRNA <69708..>70070
/gene="MCO14"
/locus_tag="YHL018W"
/product="4a-hydroxytetrahydrobiopterin dehydratase"
/transcript_id="NM_001179098.1"
/db_xref="GeneID:856368"
CDS 69708..70070
/gene="MCO14"
/locus_tag="YHL018W"
/EC_number="4.2.1.96"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095]"
/note="Putative 4a-hydroxytetrahydrobiopterin dehydratase;
green fluorescent protein (GFP)-fusion protein localizes
to mitochondria and is induced in response to the
DNA-damaging agent MMS"
/codon_start=1
/product="4a-hydroxytetrahydrobiopterin dehydratase"
/protein_id="NP_011845.1"
/db_xref="GeneID:856368"
/db_xref="SGD:S000001010"
/translation="MHNKIVRIASSALTGGKLLEKLKPLTRWEVQWDPNKTKCLGITR
EVTFKDYETTWAFLTRVSMRSHLWGHHPLIHTSYTWVKLELHTHDIDPKDGAHSQLSD
IDVRMAKRIDSYIDEMTT"
gene <70276..>71874
/locus_tag="YHL017W"
/db_xref="GeneID:856369"
mRNA <70276..>71874
/locus_tag="YHL017W"
/product="uncharacterized protein"
/transcript_id="NM_001179097.1"
/db_xref="GeneID:856369"
CDS 70276..71874
/locus_tag="YHL017W"
/experiment="EXISTENCE:direct assay:GO:0030136
clathrin-coated vesicle [PMID:14562095]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein co-localizes with clathrin-coated
vesicles; YHL017W has a paralog, PTM1, that arose from the
whole genome duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011846.1"
/db_xref="GeneID:856369"
/db_xref="SGD:S000001009"
/translation="MDRTWLLLTWLLLLCGVVQGNQESINQKYHDVCSGMYSKEDFNG
KVDPYISFTLEELSLADEDDDGEGVSVAVFDFQDYEHIGVRLPNGEIQYICDDYALDL
GLCEDSSEGQFIIQETAIDPFTSKEHKLTSQILTFTQQELGTNDKVYSINKTGYYCVT
TSSFISSSSKFKATVNFRNAYGQLDASEAYKMPIYAFLAVAYAVCTLVYSWLCWKHRH
ELLPLQRYILVFCIFLTADTIFVWMYYIIENQKGNSSVALHVYMVFISIFSAGKMTFT
LLLALLISLGYGIVYPKLDRTLLRRCQIFAIFTFAVCVAFLVQKYSQNSESLSNLILI
TAIPLVLCLFAFYYLTLSSMNKTMTYLREQNQVVKLNMYRKLIILCYISLFILFLGLL
VSTFAYVGMDTVDMIEQYWKTEFLITDTWPSFVYFLVFVIFAFFWRPTSTSYLLACSH
QLPTDMENVSEFDLDDINSLSDEALPNREPRNDYQEHDHNMDIDLASDFEEVPSVNAN
TNANNDVLFDVDYDRDAKNDRSTT"
gene complement(<72037..>74244)
/gene="DUR3"
/locus_tag="YHL016C"
/db_xref="GeneID:856370"
mRNA complement(<72037..>74244)
/gene="DUR3"
/locus_tag="YHL016C"
/product="Dur3p"
/transcript_id="NM_001179096.1"
/db_xref="GeneID:856370"
CDS complement(72037..74244)
/gene="DUR3"
/locus_tag="YHL016C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:17218313]"
/experiment="EXISTENCE:direct assay:GO:0015489 putrescine
transmembrane transporter activity [PMID:17218313]"
/experiment="EXISTENCE:direct assay:GO:0015606 spermidine
transmembrane transporter activity [PMID:17218313]"
/experiment="EXISTENCE:direct assay:GO:0015847 putrescine
transport [PMID:17218313]"
/experiment="EXISTENCE:direct assay:GO:0015848 spermidine
transport [PMID:17218313]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0005886 plasma
membrane [PMID:8335627]"
/experiment="EXISTENCE:mutant phenotype:GO:0015204 urea
transmembrane transporter activity [PMID:8335627]"
/experiment="EXISTENCE:mutant phenotype:GO:0015489
putrescine transmembrane transporter activity
[PMID:17218313]"
/experiment="EXISTENCE:mutant phenotype:GO:0015606
spermidine transmembrane transporter activity
[PMID:17218313]"
/experiment="EXISTENCE:mutant phenotype:GO:0015840 urea
transport [PMID:8335627]"
/experiment="EXISTENCE:mutant phenotype:GO:0015847
putrescine transport [PMID:17218313]"
/experiment="EXISTENCE:mutant phenotype:GO:0015848
spermidine transport [PMID:17218313]"
/note="Plasma membrane transporter for both urea and
polyamines; expression is highly sensitive to nitrogen
catabolite repression and induced by allophanate, the last
intermediate of the allantoin degradative pathway"
/codon_start=1
/product="Dur3p"
/protein_id="NP_011847.1"
/db_xref="GeneID:856370"
/db_xref="SGD:S000001008"
/translation="MGEFKPPLPQGAGYAIVLGLGAVFAGMMVLTTYLLKRYQKEIIT
AEEFTTAGRSVKTGLVAAAVVSSWIWCSTLLTSSTKEYADGIFGGYAYAAGACFQIIA
FAILAIKTKQMAPNAHTYLELVRTRYGKIGHGCYLFYAIATNILVTSMLLTSGSAVFS
DLTGMNTIASCFLLPVGVVVYTLFGGIKATFLTDYMHTCVIIIIVLVFAFKVYATSDV
LGSPGKVYDLVREAAKRHPVDGNYQGEYMTMTSKSAGILLIINLIGNFGTVFLDNGYW
NKAISASPAASLKAYAIGGLAWFAVPSLISLTMGLACLAVETSPNFPTYPDPLTSFQA
NSGLVLPAAAIAIMGKGGAVASLLMIFMAVTSAMSAELIAVSSVFTYDIYREYIDPRA
SGKKLIYTSHVACIFFGLAMSGFSVGLYYGGISMGYIYEMMGIIISSAVLPVVLTLCS
KDMNLVAAVVSPILGTGLAIMSWLVCTKSLYKELTVDTTFMDYPMLTGNLVALLSPAI
FIPILTYVFKPQNFDWEKMKDITRVDETAELVQADPDIQLYDAEANDKEQEEETNSLV
SDSEKNDVRVNNEKLIEPNLGVVISNAIFQEDDTQLQNELDEEQRELARGLKIAYFLC
VFFALAFLVVWPMPMYGSKYIFSKKFFTGWVVVMIIWLFFSAFAVCIYPLWEGRHGIY
TTLRGLYWDLSGQTYKLREWQNSNPQDLHVVTSQISARAHRQSSHFGQVDEII"
gene <74699..>74782
/locus_tag="YHL015W-A"
/db_xref="GeneID:1466525"
mRNA <74699..>74782
/locus_tag="YHL015W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184649.1"
/db_xref="GeneID:1466525"
CDS 74699..74782
/locus_tag="YHL015W-A"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878085.1"
/db_xref="GeneID:1466525"
/db_xref="SGD:S000028828"
/translation="MTAFASLREPLVLANLKIKVHIYRMKR"
gene <75412..>75777
/gene="RPS20"
/locus_tag="YHL015W"
/gene_synonym="URP2"
/db_xref="GeneID:856371"
mRNA <75412..>75777
/gene="RPS20"
/locus_tag="YHL015W"
/gene_synonym="URP2"
/product="40S ribosomal protein uS10 RPS20"
/transcript_id="NM_001179095.1"
/db_xref="GeneID:856371"
CDS 75412..75777
/gene="RPS20"
/locus_tag="YHL015W"
/gene_synonym="URP2"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0022627 cytosolic
small ribosomal subunit [PMID:3533916]"
/experiment="EXISTENCE:mutant phenotype:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16246728]"
/note="Protein component of the small (40S) ribosomal
subunit; overproduction suppresses mutations affecting RNA
polymerase III-dependent transcription; homologous to
mammalian ribosomal protein S20 and bacterial S10"
/codon_start=1
/product="40S ribosomal protein uS10 RPS20"
/protein_id="NP_011848.1"
/db_xref="GeneID:856371"
/db_xref="SGD:S000001007"
/translation="MSDFQKEKVEEQEQQQQQIIKIRITLTSTKVKQLENVSSNIVKN
AEQHNLVKKGPVRLPTKVLKISTRKTPNGEGSKTWETYEMRIHKRYIDLEAPVQIVKR
ITQITIEPGVDVEVVVASN"
gene complement(<76097..>77314)
/gene="YLF2"
/locus_tag="YHL014C"
/gene_synonym="YLF1"
/db_xref="GeneID:856372"
mRNA complement(<76097..>77314)
/gene="YLF2"
/locus_tag="YHL014C"
/gene_synonym="YLF1"
/product="Ylf2p"
/transcript_id="NM_001179094.1"
/db_xref="GeneID:856372"
CDS complement(76097..77314)
/gene="YLF2"
/locus_tag="YHL014C"
/gene_synonym="YLF1"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095|PMID:16823961|PMID:24769239]"
/note="hypothetical protein; has weak similarity to E.
coli GTP-binding protein gtp1; the authentic, non-tagged
protein is detected in highly purified mitochondria in
high-throughput studies"
/codon_start=1
/product="Ylf2p"
/protein_id="NP_011849.1"
/db_xref="GeneID:856372"
/db_xref="SGD:S000001006"
/translation="MNIGGGKFLLGRISNNPTSGIVGLANVGKSTFFQAITNSKLGNP
ANYPFATIDAECAKVNIPSVPLSNLLRIYQSAKCVPGTLTIYDIAGLTRGASQGHGLG
NKFLNDIRHVEGIFQVVRGFLKEDITHIEGNVDPVRDLSVVQDELILKDLEFLENIRE
RLSKKMRMVSKNSKEHQEMKIETELLDALEEHLFNGKKIRHFKDHWNLDEVKILNKHN
FLTSKPTLILLNVSPQDYVRNENKFVRNIIEWINEFSPGDKFLLFSAEFESQLMECKG
IASEYFDKIKEDTNVSDQQLVSAIPQIILEMRKLLNLISFFTCGPQEVHQWNIREGTT
AQEAAGVIHSDLRETFISADVIKYDDLKKMEPPLNESLLKSKGLIKRAGKQYIMQDND
IALFKAAGGKIKK"
gene complement(<77430..>78353)
/gene="OTU2"
/locus_tag="YHL013C"
/db_xref="GeneID:856373"
mRNA complement(<77430..>78353)
/gene="OTU2"
/locus_tag="YHL013C"
/product="deubiquitinase OTU2"
/transcript_id="NM_001179093.1"
/db_xref="GeneID:856373"
CDS complement(77430..78353)
/gene="OTU2"
/locus_tag="YHL013C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="hypothetical protein; may interact with ribosomes,
based on co-purification experiments; member of the
ovarian tumor-like (OTU) superfamily of predicted cysteine
proteases; shows cytoplasmic localization; protein
abundance increases in response to DNA replication stress"
/codon_start=1
/product="deubiquitinase OTU2"
/protein_id="NP_011850.1"
/db_xref="GeneID:856373"
/db_xref="SGD:S000001005"
/translation="MTGMESGENLENMEDILARHRKENKDLQNKITGMKKQATKSKRK
EVNSKCLDLQDKLKTKQENEIRDWKIANNEVFDAEQEDEVTPEKLLEQLSISRDEKEQ
QNVPVQQQQQGQTKKRRNRQKERLAKRDAAIAKMKEEAALEASKQPDLKKMEQESIDQ
LCELKKLKQFDIQPDGHCLFASILDQLKLRHDPKKLDQDMDVMKLRWLSCNYVQEHRD
DFIPYLFDEETMKMKDIDEYTKEMEHTAQWGGEIEILALSHVFDCPISILMSGRPIQV
YNECGKNPELKLVYYKHSYALGEHYNSLHDS"
gene <78936..>80417
/locus_tag="YHL012W"
/db_xref="GeneID:856374"
mRNA <78936..>80417
/locus_tag="YHL012W"
/product="UTP--glucose-1-phosphate uridylyltransferase"
/transcript_id="NM_001179092.1"
/db_xref="GeneID:856374"
CDS 78936..80417
/locus_tag="YHL012W"
/EC_number="2.7.7.9"
/note="Putative UTP glucose-1-phosphate
uridylyltransferase; YHL012W has a paralog, UGP1, that
arose from the whole genome duplication"
/codon_start=1
/product="UTP--glucose-1-phosphate uridylyltransferase"
/protein_id="NP_011851.1"
/db_xref="GeneID:856374"
/db_xref="SGD:S000001004"
/translation="MTVFSGVNKIEFEGTFEGIGKDVVMSQMIRALQKHFPSIRDKNY
EFSLFLHIFQRYVLENTSITHDLVCDKIRLPIIDEVVELDDIKNYGLLEGKLLSKLAI
LKLTGKANPIIGKESPLFEVKNGMSSLDVIVRQTQNLNVRYNSDVPLIFMTSLETESQ
VSNFLEEHYSSSKVRWKTVVQSSFPQIDKDRLLPIDLQINSHENDFWYPCGTGNLTDT
LYFSGELDKLIAQGKEILFVSNVDNLGATGDLNILNFIINEKIEYLVEVVERTANVSN
TGVLATYKGKLRSVYYNCLSNESASTCRIVNTNNIWIDLKKLKVLIESNSLNLPIHSS
ESKITHKNEEIECLQFKTQLVDCIAFFPNSRVLKVSRDRFLPLRTCKDLFLLKSTLYD
LDSNGTFNLYPLKFGLLPSIDLGDEFATYETFKIGVPDIPNILELEHLTVMGNVFFGR
NITLKGTVIIICDENDVITVPDGSILENVTIWHKSQLEDMNGY"
gene complement(<80654..>81616)
/gene="PRS3"
/locus_tag="YHL011C"
/db_xref="GeneID:856375"
mRNA complement(<80654..>81616)
/gene="PRS3"
/locus_tag="YHL011C"
/product="ribose phosphate diphosphokinase subunit PRS3"
/transcript_id="NM_001179091.1"
/db_xref="GeneID:856375"
CDS complement(80654..81616)
/gene="PRS3"
/locus_tag="YHL011C"
/EC_number="2.7.6.1"
/experiment="EXISTENCE:direct assay:GO:0002189 ribose
phosphate diphosphokinase complex [PMID:15280369]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0004749
ribose phosphate diphosphokinase activity [PMID:15280369]"
/experiment="EXISTENCE:genetic interaction:GO:0006015
5-phosphoribose 1-diphosphate biosynthetic process
[PMID:15280369]"
/experiment="EXISTENCE:mutant phenotype:GO:0006015
5-phosphoribose 1-diphosphate biosynthetic process
[PMID:10212224]"
/note="5-phospho-ribosyl-1(alpha)-pyrophosphate
synthetase; synthesizes PRPP, which is required for
nucleotide, histidine, and tryptophan biosynthesis; one of
five related enzymes, which are active as heteromultimeric
complexes"
/codon_start=1
/product="ribose phosphate diphosphokinase subunit PRS3"
/protein_id="NP_011852.1"
/db_xref="GeneID:856375"
/db_xref="SGD:S000001003"
/translation="MPTNSIKLLAPDVHRGLAELVAKRLGLQLTSSKLKRDPTGEVSF
SIGESVRDQDIFIITQIGSGVVNDRVLELLIMINASKTASARRITAIIPNFPYARQDR
KDKSRAPITAKLMADMLTTAGCDHVITMDLHASQIQGFFDVPVDNLYAEPSVVRYIKE
NVNYMDSIIISPDAGGAKRAATLADRLDLNFALIHKERARANEVSRMVLVGDVTDKIC
IIVDDMADTCGTLAKAAEILLENRAKSVIAIVTHGVLSGRAIENINNSKLDRVVCTNT
VPFEEKIKKCPKLAVIDISSVLAESIRRLHNGESISYLFKNYPL"
gene complement(<81964..>83721)
/gene="ETP1"
/locus_tag="YHL010C"
/db_xref="GeneID:856376"
mRNA complement(<81964..>83721)
/gene="ETP1"
/locus_tag="YHL010C"
/product="Etp1p"
/transcript_id="NM_001179090.1"
/db_xref="GeneID:856376"
CDS complement(81964..83721)
/gene="ETP1"
/locus_tag="YHL010C"
/experiment="EXISTENCE:direct assay:GO:0008139 nuclear
localization sequence binding [PMID:9497340]"
/experiment="EXISTENCE:direct assay:GO:0043130 ubiquitin
binding [PMID:16564012]"
/experiment="EXISTENCE:mutant phenotype:GO:0045471
response to ethanol [PMID:19416103]"
/note="hypothetical protein required for growth on
ethanol; contains a zinc finger region and has homology to
human BRAP2, which is a cytoplasmic protein that binds
nuclear localization sequences"
/codon_start=1
/product="Etp1p"
/protein_id="NP_011853.1"
/db_xref="GeneID:856376"
/db_xref="SGD:S000001002"
/translation="MDQFEYIITLEFGNQNQVESAYQIFKSIPKKLKAKSIGEESIKS
NNQDWQDWRVCDLEIDMITDFKNQTSKEEESDLITSQYLGHGIIRLFKLSNANNTLNE
KEILTIPGDDTMICILFVPTYFTVHDLLHFYIGDDIVNKQVSNFRILRNQQKGMGFNF
TVLIKFRNALDAKNFKEEFNGKSFSRMDPETCHVISVKEIVFQKKLFQRPAANEDFPY
LLTDPFTVKKKKELVKVELPTCPVCLERMDSETTGLVTIPCQHTFHCQCLNKWKNSRC
PVCRHSSLRLSRESLLKQAGDSAHCATCGSTDNLWICLICGNVGCGRYNSKHAIKHYE
ETLHCFAMDIRTQRVWDYAGDNYVHRLVQNEVDGKLVEVGGSGDDDNNDIGNSDELQN
VVYGNRSKNGEKSNSNKKDGELAANFLRHREYHLEYVQVLISQLESQREYYELKLQEK
DQTASDSSNVESLKKSMEDLKLQFQVTQKEWQKREMAQKSKLEEDMLVIEGLQANLDH
LSKKQEQLERENKALEESKQDLEEQVKDLMFYLDSQEKFKDADESVKEGTILIQQPHG
AAQASKSKKKRNKNKKAGK"
gene complement(<84068..>85060)
/gene="YAP3"
/locus_tag="YHL009C"
/db_xref="GeneID:856377"
mRNA complement(<84068..>85060)
/gene="YAP3"
/locus_tag="YHL009C"
/product="Yap3p"
/transcript_id="NM_001179089.1"
/db_xref="GeneID:856377"
CDS complement(84068..85060)
/gene="YAP3"
/locus_tag="YHL009C"
/experiment="EXISTENCE:curator inference:GO:0005634
nucleus [PMID:9372930]"
/experiment="EXISTENCE:direct assay:GO:0003700 DNA-binding
transcription factor activity [PMID:9372930]"
/experiment="EXISTENCE:direct assay:GO:0006357 regulation
of transcription by RNA polymerase II [PMID:9372930]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:19111667]"
/note="Basic leucine zipper (bZIP) transcription factor"
/codon_start=1
/product="Yap3p"
/protein_id="NP_011854.1"
/db_xref="GeneID:856377"
/db_xref="SGD:S000001001"
/translation="MTPSNMDDNTSGFMKFINPQCQEEDCCIRNSLFQEDSKCIKQQP
DLLSEQTAPFPILEDQCPALNLDRSNNDLLLQNNISFPKGSDLQAIQLTPISGDYSTY
VMADNNNNDNDSYSNTNYFSKNNGISPSSRSPSVAHNENVPDDSKAKKKAQNRAAQKA
FRERKEARMKELQDKLLESERNRQSLLKEIEELRKANTEINAENRLLLRSGNENFSKD
IEDDTNYKYSFPTKDEFFTSMVLESKLNHKGKYSLKDNEIMKRNTQYTDEAGRHVLTV
PATWEYLYKLSEERDFDVTYVMSKLQGQECCHTHGPAYPRSLIDFLVEEATLNE"
gene complement(85298..85371)
/locus_tag="YNCH0002C"
/db_xref="GeneID:856378"
tRNA complement(85298..85371)
/locus_tag="YNCH0002C"
/product="tRNA-Val"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Valine tRNA (tRNA-Val), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:856378"
/db_xref="SGD:S000006758"
repeat_region complement(85388..85538)
/note="Ty3 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006987"
mobile_element 85539..91761
/note="YHLWTy4-1; Ty4 element, LTR retrotransposon of the
Copia (Pseudoviridae) group; contains co-transcribed genes
TYA Gag and TYB Pol, encoding proteins involved in
structure and function of virus-like particles, flanked by
two direct repeats"
/mobile_element_type="retrotransposon:YHLWTy4-1"
/db_xref="SGD:S000006991"
repeat_region 85539..85909
/note="Ty4 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006989"
gene <85909..>91318
/locus_tag="YHL009W-B"
/db_xref="GeneID:856380"
mRNA <85909..>91318
/locus_tag="YHL009W-B"
/product="gag-pol fusion protein"
/transcript_id="NM_001184404.2"
/db_xref="GeneID:856380"
CDS join(85909..86994,86996..91318)
/locus_tag="YHL009W-B"
/EC_number="2.7.7.7"
/EC_number="2.7.7.49"
/EC_number="3.1.26.4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9448009]"
/ribosomal_slippage
/note="Retrotransposon TYA Gag and TYB Pol genes;
transcribed/translated as one unit; polyprotein is
processed to make a nucleocapsid-like protein (Gag),
reverse transcriptase (RT), protease (PR), and integrase
(IN); similar to retroviral genes"
/codon_start=1
/product="gag-pol fusion protein"
/protein_id="NP_058133.1"
/db_xref="GeneID:856380"
/db_xref="SGD:S000007372"
/translation="MATPVRDETRNVIDDNISARIQSKVKTNDTVRQTPSSLRKVSIK
DEQVKQYQRNLNRFKTILNGLKAEEEKLSETDDIQMLAEKLLKLGETIDKVENRIVDL
VEKIQLLETNENNNILHEHIDATGTYYLFDTLTSTNKRFYPKDCVFDYRTNNVENIPI
LLNNFKKFIKKYQFDDVFENDIIEIDPRENEILCKIIKEGLGESLDIMNTNTTDIFRI
IDGLKNKYRSLHGRDVRIRAWEKVLVDTTCRNSALLMNKLQKLVLMEKWIFSKCCQDC
PNLKDYLQEAIMGTLHESLRNSVKQRLYNIPHNVGINHEEFLINTVIETVIDLSPIAD
DQIENSCMYCKSVFHCSINCKKKPNRELGLTRPISQKPIIYKVHRDNNNLSPVQNEQK
SWNKTQKKSNKVYNSKKLVIIDTGSGVNITNDKTLLHNYEDSNRSTRFFGIGKNSSVS
VKGYGYIKIKNGHNNTDNKCLLTYYVPEEESTIISCYDLAKKTKMVLSRKYTRLGNKI
IKIKTKIVNGVIHVKMNELIERPSDDSKINAIKPTSSPGFKLNKRSITLEDAHKRMGH
TGIQQIENSIKHNHYEESLDLIKEPNEFWCQTCKISKATKRNHYTGSMNNHSTDHEPG
SSWCMDIFGPVSSSNADTKRYMLIMVDNNTRYCMTSTHFNKNAETILAQIRKNIQYVE
TQFDRKVREINSDRGTEFTNDQIEEYFISKGIHHILTSTQDHAANGRAERYIRTIVTD
ATTLLRQSNLRVKFWEYAVTSATNIRNCLEHKSTGKLPLKAISRQPVTVRLMSFLPFG
EKGIIWNHNHKKLKPSGLPSIILCKDPNSYGYKFFIPSKNKIVTSDNYTIPNYTMDGR
VRNTQNIYKSHQFSSHNDNEEDQIETVTNLCEALENYEDDNKPITRLEDLFTEEELSQ
IDSNAKYPSPSNNLEGDLDYVFSDVEESGDYDVESELSTTNTSISTDKNKILSNKDFN
SELASTEISISEIDKKGLINTSHIDEDKYDEKVHRIPSIIQEKLVGSKNTIKINDENR
ISDRIRSKNIGSILNTGLSRCVDITDESITNKDESMHNAKPELIQEQFNKTNHETSFP
KEGSIGTNVKFRNTDNEISLKTGDTSLPIKTLESINNHHSNDYSTNKVEKFEKENHHP
PPIEDIVDMSDQTDMESNCQDGNNLKELKVTDKNVPTDNGTNVSPRLEQNIEASGSPV
QTVNKSAFLNKEFSSLNMKRKRKRHDKNNSLTSYELERDKKRSKRNRVKLIPDNMETV
SAQKIRAIYYNEAISKNPDLKEKHEYKQAYHKELQNLKDMKVFDVDVKYSRSEIPDNL
IVPTNTIFTKKRNGIYKARIVCRGDTQSPDTYSVITTESLNHNHIKIFLMIANNRNMF
MKTLDINHAFLYAKLEEEIYIPHPHDRRCVVKLNKALYGLKQSPKEWNDHLRQYLNGI
GLKDNSYTPGLYQTEDKNLMIAVYVDDCVIAASNEQRLDEFINKLKSNFELKITGTLI
DDVLDTDILGMDLVYNKRLGTIDLTLKSFINRMDKKYNEELKKIRKSSIPHMSTYKID
PKKDVLQMSEEEFRQGVLKLQQLLGELNYVRHKCRYDINFAVKKVARLVNYPHERVFY
MIYKIIQYLVRYKDIGIHYDRDCNKDKKVIAITDASVGSEYDAQSRIGVILWYGMNIF
NVYSNKSTNRCVSSTEAELHAIYEGYADSETLKVTLKELGEGDNNDIVMITDSKPAIQ
GLNRSYQQPKEKFTWIKTEIIKEKIKEKSIKLLKITGKGNIADLLTKPVSASDFKRFI
QVLKNKITSQDILASTDY"
gene <85909..>87150
/locus_tag="YHL009W-A"
/db_xref="GeneID:856379"
mRNA <85909..>87150
/locus_tag="YHL009W-A"
/product="gag protein"
/transcript_id="NM_001184403.1"
/db_xref="GeneID:856379"
CDS 85909..87150
/locus_tag="YHL009W-A"
/note="Retrotransposon TYA Gag gene co-transcribed with
TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is
a nucleocapsid protein that is the structural constituent
of virus-like particles (VLPs); similar to retroviral Gag"
/codon_start=1
/product="gag protein"
/protein_id="NP_058134.1"
/db_xref="GeneID:856379"
/db_xref="SGD:S000007371"
/translation="MATPVRDETRNVIDDNISARIQSKVKTNDTVRQTPSSLRKVSIK
DEQVKQYQRNLNRFKTILNGLKAEEEKLSETDDIQMLAEKLLKLGETIDKVENRIVDL
VEKIQLLETNENNNILHEHIDATGTYYLFDTLTSTNKRFYPKDCVFDYRTNNVENIPI
LLNNFKKFIKKYQFDDVFENDIIEIDPRENEILCKIIKEGLGESLDIMNTNTTDIFRI
IDGLKNKYRSLHGRDVRIRAWEKVLVDTTCRNSALLMNKLQKLVLMEKWIFSKCCQDC
PNLKDYLQEAIMGTLHESLRNSVKQRLYNIPHNVGINHEEFLINTVIETVIDLSPIAD
DQIENSCMYCKSVFHCSINCKKKPNRELRPDSTNFSKTYYLQGAQRQQQLKSSAKRTK
VLEQDTKKVKQSVQQQKTGNY"
repeat_region 91391..91761
/note="Ty4 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006990"
repeat_region complement(91772..92099)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006985"
repeat_region 92240..92390
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006988"
gene complement(<92627..>94510)
/locus_tag="YHL008C"
/db_xref="GeneID:856381"
mRNA complement(<92627..>94510)
/locus_tag="YHL008C"
/product="uncharacterized protein"
/transcript_id="NM_001179088.1"
/db_xref="GeneID:856381"
CDS complement(92627..94510)
/locus_tag="YHL008C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0006821
chloride transport [PMID:18378800]"
/experiment="EXISTENCE:mutant phenotype:GO:0140299
molecular sensor activity [PMID:18378800]"
/experiment="EXISTENCE:mutant phenotype:GO:2001225
regulation of chloride transport [PMID:18378800]"
/note="hypothetical protein; may be involved in the uptake
of chloride ions; does not appear to be involved in
monocarboxylic acid transport; green fluorescent protein
(GFP)-fusion protein localizes to the vacuole"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011855.1"
/db_xref="GeneID:856381"
/db_xref="SGD:S000001000"
/translation="MVDDSNYLTPHETALAVVATAMKKARLQLDTLLINSILGGVLFS
SGSFLLVAVYSEDPDIVARNPGIVNLITGVNFAMGLFYVVMMGADLFNSNILFFSVGV
LRKAVTIYDLMISWVVSWLGNIAGSLFVSYLFGHLSGISSQKLWIIGSRQIIEQKVSY
SFVQTFLKGIACNFFVCLAIYLQLMAKPIHVKFILMSFPIIDFIGIGFTHVVGDMSAS
FIAMLNGANVSVGKYIWKLLIPASLGNIVGGLFFSAVVPFYLHLVVVERDRKRLSLPE
YEARDEQPELNMDSRVVRIQKNECDDDATETGEDLENLTEKGFASIYNTNHDNSSYFT
GRSLNSLRSIPSSVITSDNVTMESDLGEPVQFIPKSNSTTRSPHLGLPHNLPHNHSIK
SINRHRINKRHSLRSPPGVFPVRGMGEPLEREKTIEDATYDPKENELFLRRAETHNSA
YVKNKKKEDDNLLRLVKTEEDREQKEYEKNGGYNILENKPGTRLEKIITHLAENVSSR
EVTPPILPRTTQDTFPHNAPASSPAYTDDAHSLRKANSTTLGGLFRAVSKEFHSSKDA
ESPDDLLKKMAAVGINRNARITANNVAGIVNLNKEDLDSTTRRQKITEPKNFYNRHTS
PQL"
gene complement(<95118..>97937)
/gene="STE20"
/locus_tag="YHL007C"
/db_xref="GeneID:856382"
mRNA complement(<95118..>97937)
/gene="STE20"
/locus_tag="YHL007C"
/product="mitogen-activated protein kinase kinase kinase
kinase STE20"
/transcript_id="NM_001179087.1"
/db_xref="GeneID:856382"
CDS complement(95118..97937)
/gene="STE20"
/locus_tag="YHL007C"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:9003780]"
/experiment="EXISTENCE:direct assay:GO:0000165 MAPK
cascade [PMID:7608157]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:19793923|PMID:15652479]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836]"
/experiment="EXISTENCE:direct assay:GO:0008349 MAP kinase
kinase kinase kinase activity [PMID:7608157]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:9003780]"
/experiment="EXISTENCE:direct assay:GO:0044025 histone
H2BS14 kinase activity [PMID:15652479]"
/experiment="EXISTENCE:direct assay:GO:0070301 cellular
response to hydrogen peroxide [PMID:15652479]"
/experiment="EXISTENCE:genetic interaction:GO:0000011
vacuole inheritance [PMID:19218422]"
/experiment="EXISTENCE:genetic interaction:GO:0000750
pheromone-dependent signal transduction involved in
conjugation with cellular fusion [PMID:1464311]"
/experiment="EXISTENCE:genetic interaction:GO:0007118
budding cell apical bud growth [PMID:10866679]"
/experiment="EXISTENCE:genetic interaction:GO:0007121
bipolar cellular bud site selection [PMID:10866679]"
/experiment="EXISTENCE:genetic interaction:GO:0007232
osmosensory signaling pathway via Sho1 osmosensor
[PMID:10970855]"
/experiment="EXISTENCE:genetic interaction:GO:0010629
negative regulation of gene expression [PMID:19793923]"
/experiment="EXISTENCE:genetic interaction:GO:0034063
stress granule assembly [PMID:20513766]"
/experiment="EXISTENCE:mutant phenotype:GO:0000011 vacuole
inheritance [PMID:19218422]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:19793923]"
/experiment="EXISTENCE:mutant phenotype:GO:0000282
cellular bud site selection [PMID:11452010]"
/experiment="EXISTENCE:mutant phenotype:GO:0000750
pheromone-dependent signal transduction involved in
conjugation with cellular fusion [PMID:1464311]"
/experiment="EXISTENCE:mutant phenotype:GO:0001402 signal
transduction involved in filamentous growth
[PMID:8259520]"
/experiment="EXISTENCE:mutant phenotype:GO:0001403
invasive growth in response to glucose limitation
[PMID:8001818]"
/experiment="EXISTENCE:mutant phenotype:GO:0005634 nucleus
[PMID:19793923]"
/experiment="EXISTENCE:mutant phenotype:GO:0007096
regulation of exit from mitosis [PMID:12234925]"
/experiment="EXISTENCE:mutant phenotype:GO:0007118 budding
cell apical bud growth [PMID:10866679]"
/experiment="EXISTENCE:mutant phenotype:GO:0007121 bipolar
cellular bud site selection [PMID:10866679]"
/experiment="EXISTENCE:mutant phenotype:GO:0007124
pseudohyphal growth [PMID:8259520]"
/experiment="EXISTENCE:mutant phenotype:GO:0007232
osmosensory signaling pathway via Sho1 osmosensor
[PMID:10970855]"
/experiment="EXISTENCE:mutant phenotype:GO:0010629
negative regulation of gene expression [PMID:19793923]"
/experiment="EXISTENCE:mutant phenotype:GO:0034063 stress
granule assembly [PMID:20513766]"
/experiment="EXISTENCE:mutant phenotype:GO:0035376 sterol
import [PMID:19793923]"
/experiment="EXISTENCE:mutant phenotype:GO:0043065
positive regulation of apoptotic process [PMID:15652479]"
/experiment="EXISTENCE:mutant phenotype:GO:2000910
negative regulation of sterol import [PMID:19793923]"
/experiment="EXISTENCE:physical interaction:GO:0000749
response to pheromone triggering conjugation with cellular
fusion [PMID:9428767]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:9428767]"
/note="Cdc42p-activated signal transducing kinase;
involved in pheromone response, pseudohyphal/invasive
growth, vacuole inheritance, down-regulation of sterol
uptake; GBB motif binds Ste4p; member of the PAK
(p21-activated kinase) family"
/codon_start=1
/product="mitogen-activated protein kinase kinase kinase
kinase STE20"
/protein_id="NP_011856.1"
/db_xref="GeneID:856382"
/db_xref="SGD:S000000999"
/translation="MSNDPSAVSELPDKDSLDNGISNDNERAMGGNGDGGDGLRLPRT
TGTLNVNALQKGTNAAHEAGGYKSMDPAKNAETTNDDDNNVVSLDDPIQFTRVSSSSV
ISGMSSSMSPHSNIDETKSLEAVTPNINTSNITPDHSADNTFSTINASESDHQFNDTL
LSKLSLTDSTETIENNATVKHQQPVASSTVNSNKSSTDIRRATPVSTPVISKPSMTTT
PRQINSASHSLSNPKHKQHKPKVKPSKPEAKSKPVSVKKSFPSKNPLKNSSPPKKQTE
KSYYSSSSKKRKSGSNSGTLRMKDVFTSFVQNIKRNSQDDKRASSSSNNSSSSSITTA
LRISTPYNAKHIHHVGVDSKTGEYTGLPEEWEKLLTSSGISKREQQQNMQAVMDIVKF
YQDVTETNGEDKMFKTFNTTTGLPGSPQVSTPPANSFNKFPPSTSDSHNYGSRTGTPM
SNHVMSPTLNTDSSSANGKFIPSRPAPKPPSSASASAPIIKSPVMNSAANVSPLKQTH
APTTPNRTSPNRSSISRNATLKKEEQPLPPIPPTKSKTSPIISTAHTPQQVAQSPKAP
AQETVTTPTSKPAQARSLSKELNEKKREERERRKKQLYAKLNEICSDGDPSTKYANLV
KIGQGASGGVYTAYEIGTNVSVAIKQMNLEKQPKKELIINEILVMKGSKHPNIVNFID
SYVLKGDLWVIMEYMEGGSLTDVVTHCILTEGQIGAVCRETLSGLEFLHSKGVLHRDI
KSDNILLSMEGDIKLTDFGFCAQINELNLKRTTMVGTPYWMAPEVVSRKEYGPKVDIW
SLGIMIIEMIEGEPPYLNETPLRALYLIATNGTPKLKEPENLSSSLKKFLDWCLCVEP
EDRASATELLHDEYITEIAEANSSLAPLVKLARLKKVAENMDADEDNDDDNDNEHINK
TNNCDDNNDSKETVNLDVTEDDKQK"
gene complement(<98343..>98795)
/gene="SHU1"
/locus_tag="YHL006C"
/db_xref="GeneID:856383"
mRNA complement(<98343..>98795)
/gene="SHU1"
/locus_tag="YHL006C"
/product="Shu1p"
/transcript_id="NM_001179086.1"
/db_xref="GeneID:856383"
CDS complement(98343..98795)
/gene="SHU1"
/locus_tag="YHL006C"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:21372173]"
/experiment="EXISTENCE:direct assay:GO:0097196 Shu complex
[PMID:23575680]"
/experiment="EXISTENCE:genetic interaction:GO:0000725
recombinational repair [PMID:15654096]"
/experiment="EXISTENCE:genetic interaction:GO:0043007
maintenance of rDNA [PMID:21372173]"
/experiment="EXISTENCE:mutant phenotype:GO:0000725
recombinational repair [PMID:23575680]"
/experiment="EXISTENCE:mutant phenotype:GO:0000730 DNA
recombinase assembly [PMID:23575680]"
/experiment="EXISTENCE:mutant phenotype:GO:0035861 site of
double-strand break [PMID:23575680]"
/experiment="EXISTENCE:physical interaction:GO:0097196 Shu
complex [PMID:15654096]"
/note="Component of Shu complex (aka PCSS complex); Shu
complex also includes Psy3, Csm2, Shu2, and promotes
error-free DNA repair, mediates inhibition of Srs2p
function; essential for promoting the establishment of
homolog bias during meiotic homologous recombination;
promotes both crossover (CO) and non-crossover (NCO)
pathways of meiotic recombination and formation of Rad51p
filaments"
/codon_start=1
/product="Shu1p"
/protein_id="NP_011857.2"
/db_xref="GeneID:856383"
/db_xref="SGD:S000000998"
/translation="MQFEERLQQLVESDWSLDQSSPNVLVIVLGDTARKYVELGGLKE
HVTTNTVAGHVASRERVSVVFLGRVKYLYMYLTRMQAQANGPQYSNVLVYGLWDLTAT
QDGPQQLRLLSLVLRQCLSLPSKVEFYPEPPSSSVPARLLRFWDHIIR"
gene complement(<98828..>99220)
/locus_tag="YHL005C"
/db_xref="GeneID:856385"
mRNA complement(<98828..>99220)
/locus_tag="YHL005C"
/product="uncharacterized protein"
/transcript_id="NM_001348872.1"
/db_xref="GeneID:856385"
CDS complement(98828..99220)
/locus_tag="YHL005C"
/note="hypothetical protein; conserved among S. cerevisiae
strains; YHL005C is not an essential gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335738.1"
/db_xref="GeneID:856385"
/db_xref="SGD:S000000997"
/translation="MRKESFLTFYFSNHLYLCPAIIRLSSVCTLARTDYYLPSNIAVT
YDIQISSLGFTYRIDFFLALFSDPARPFLTEINRKIGQYACVIREREQAGEYSFHYSL
CININVYILHIHIYIDRYIYAYINAQVQ"
gene <99219..>100403
/gene="MRP4"
/locus_tag="YHL004W"
/gene_synonym="uS2m"
/db_xref="GeneID:856384"
mRNA <99219..>100403
/gene="MRP4"
/locus_tag="YHL004W"
/gene_synonym="uS2m"
/product="mitochondrial 37S ribosomal protein uS2m MRP4"
/transcript_id="NM_001179084.1"
/db_xref="GeneID:856384"
CDS 99219..100403
/gene="MRP4"
/locus_tag="YHL004W"
/gene_synonym="uS2m"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:11278769]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:11278769]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005763
mitochondrial small ribosomal subunit [PMID:11278769]"
/note="Mitochondrial ribosomal protein of the small
subunit"
/codon_start=1
/product="mitochondrial 37S ribosomal protein uS2m MRP4"
/protein_id="NP_011859.1"
/db_xref="GeneID:856384"
/db_xref="SGD:S000000996"
/translation="MQRHVFARNFRRLSLLRNPSLTKRFQSSASGAANTPNNNDEVML
LQQKLLYDEIRSELKSLSQVPEDEILPELKKSLEQDKLSDKEQQLEAELSDFFRNYAL
LNKLFDSKTLDGQSSTTTAAATPTKPYPNLIPSANDKPYSSQELFLRQLNHSMRTAKL
GATISKVYYPHKDIFYPPLPENITVESLMSAGVHLGQSTSLWRSSTQSYIYGEYKGIH
IIDLNQTLSYLKRAAKVVEGVSESGGIILFLGTRQGQKRGLEEAAKKTHGYYVSTRWI
PGTLTNSTEISGIWEKQEIDSNDNPTERALSPNETSKQVKPDLLVVLNPTENRNALLE
AIKSRVPTIAIIDTDSEPSLVTYPIPGNDDSLRSVNFLLGVLARAGQRGLQNRLARNN
EK"
gene complement(<100648..>101883)
/gene="LAG1"
/locus_tag="YHL003C"
/db_xref="GeneID:856386"
mRNA complement(<100648..>101883)
/gene="LAG1"
/locus_tag="YHL003C"
/product="sphingosine N-acyltransferase LAG1"
/transcript_id="NM_001179083.1"
/db_xref="GeneID:856386"
CDS complement(100648..101883)
/gene="LAG1"
/locus_tag="YHL003C"
/EC_number="2.3.1.297"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum
[PMID:26928762|PMID:22842922|PMID:14562095|PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0046513 ceramide
biosynthetic process [PMID:15692566]"
/experiment="EXISTENCE:direct assay:GO:0050291 sphingosine
N-acyltransferase activity [PMID:15692566]"
/experiment="EXISTENCE:direct assay:GO:0061576 acyl-CoA
ceramide synthase complex [PMID:15692566]"
/experiment="EXISTENCE:mutant phenotype:GO:0046513
ceramide biosynthetic process [PMID:11694577]"
/experiment="EXISTENCE:mutant phenotype:GO:0050291
sphingosine N-acyltransferase activity
[PMID:11694577|PMID:11387200]"
/note="Ceramide synthase component; involved in synthesis
of ceramide from C26(acyl)-coenzyme A and
dihydrosphingosine or phytosphingosine, functionally
equivalent to Lac1p; forms ER foci upon DNA replication
stress; homolog of human CERS2, a tumor metastasis
suppressor gene whose silencing enhances
invasion/metastasis of prostate cancer cells; LAG1 has a
paralog, LAC1, that arose from the whole genome
duplication"
/codon_start=1
/product="sphingosine N-acyltransferase LAG1"
/protein_id="NP_011860.1"
/db_xref="GeneID:856386"
/db_xref="SGD:S000000995"
/translation="MTSATDKSIDRLVVNAKTRRRNSSVGKIDLGDTVPGFAAMPESA
ASKNEAKKRMKALTGDSKKDSDLLWKVWFSYREMNYRHSWLTPFFILVCVYSAYFLSG
NRTESNPLHMFVAISYQVDGTDSYAKGIKDLSFVFFYMIFFTFLREFLMDVVIRPFTV
YLNVTSEHRQKRMLEQMYAIFYCGVSGPFGLYIMYHSDLWLFKTKPMYRTYPVITNPF
LFKIFYLGQAAFWAQQACVLVLQLEKPRKDYKELVFHHIVTLLLIWSSYVFHFTKMGL
AIYITMDVSDFFLSLSKTLNYLNSVFTPFVFGLFVFFWIYLRHVVNIRILWSVLTEFR
HEGNYVLNFATQQYKCWISLPIVFVLIAALQLVNLYWLFLILRILYRLIWQGIQKDER
SDSDSDESAENEESKEKCE"
gene <102612..>103970
/gene="HSE1"
/locus_tag="YHL002W"
/db_xref="GeneID:856387"
mRNA <102612..>103970
/gene="HSE1"
/locus_tag="YHL002W"
/product="ESCRT-0 subunit protein HSE1"
/transcript_id="NM_001179082.1"
/db_xref="GeneID:856387"
CDS 102612..103970
/gene="HSE1"
/locus_tag="YHL002W"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:12055639]"
/experiment="EXISTENCE:direct assay:GO:0005774 vacuolar
membrane [PMID:31740006]"
/experiment="EXISTENCE:direct assay:GO:0043130 ubiquitin
binding [PMID:20150893|PMID:12055639]"
/experiment="EXISTENCE:mutant phenotype:GO:0006623 protein
targeting to vacuole [PMID:12055639]"
/experiment="EXISTENCE:mutant phenotype:GO:0016237
microautophagy [PMID:31740006]"
/experiment="EXISTENCE:mutant phenotype:GO:0045324 late
endosome to vacuole transport [PMID:12055639]"
/experiment="EXISTENCE:mutant phenotype:GO:1904669 ATP
export [PMID:26585826]"
/experiment="EXISTENCE:physical interaction:GO:0006623
protein targeting to vacuole [PMID:12055639]"
/experiment="EXISTENCE:physical interaction:GO:0033565
ESCRT-0 complex [PMID:12055639]"
/note="Subunit of the endosomal Vps27p-Hse1p complex;
complex is required for sorting of ubiquitinated membrane
proteins into intralumenal vesicles prior to vacuolar
degradation, as well as for recycling of Golgi proteins
and formation of lumenal membranes"
/codon_start=1
/product="ESCRT-0 subunit protein HSE1"
/protein_id="NP_011861.1"
/db_xref="GeneID:856387"
/db_xref="SGD:S000000994"
/translation="MSSSAIKIRNALLKATDPKLRSDNWQYILDVCDLVKEDPEDNGQ
EVMSLIEKRLEQQDANVILRTLSLTVSLAENCGSRLRQEISSKNFTSLLYALIESHSV
HITLKKAVTDVVKQLSDSFKDDPSLRAMGDLYDKIKRKAPYLVQPNVPEKHNMSTQAD
NSDDEELQKALKMSLFEYEKQKKLQEQEKESAEVLPQQQQQHQQQNQAPAHKIPAQTV
VRRVRALYDLTTNEPDELSFRKGDVITVLEQVYRDWWKGALRGNMGIFPLNYVTPIVE
PSKEEIEKEKNKEAIVFSQKTTIDQLHNSLNAASKTGNSNEVLQDPHIGDMYGSVTPL
RPQVTRMLGKYAKEKEDMLSLRQVLANAERSYNQLMDRAANAHISPPVPGPALYAGMT
HANNTPVMPPQRQSYQSNEYSPYPSNLPIQHPTNSANNTPQYGYDLGYSVVSQPPPGY
EQ"
gene <104277..>105091
/gene="RPL14B"
/locus_tag="YHL001W"
/db_xref="GeneID:856388"
mRNA join(<104277..104405,104804..>105091)
/gene="RPL14B"
/locus_tag="YHL001W"
/product="60S ribosomal protein eL14 RPL14B"
/transcript_id="NM_001179081.1"
/db_xref="GeneID:856388"
CDS join(104277..104405,104804..105091)
/gene="RPL14B"
/locus_tag="YHL001W"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:11983894]"
/experiment="EXISTENCE:curator inference:GO:0003735
structural constituent of ribosome [PMID:11983894]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:6337137]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:29788267]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:11983894]"
/experiment="EXISTENCE:mutant phenotype:GO:0000027
ribosomal large subunit assembly [PMID:29788267]"
/experiment="EXISTENCE:mutant phenotype:GO:0000470
maturation of LSU-rRNA [PMID:29788267]"
/note="Ribosomal 60S subunit protein L14B; homologous to
mammalian ribosomal protein L14, no bacterial homolog;
RPL14B has a paralog, RPL14A, that arose from the whole
genome duplication; protein abundance increases in
response to DNA replication stress"
/codon_start=1
/product="60S ribosomal protein eL14 RPL14B"
/protein_id="NP_011862.1"
/db_xref="GeneID:856388"
/db_xref="SGD:S000000993"
/translation="MSTDSIVKASNWRLVEVGRVVLIKKGQSAGKLAAIVEIIDQKKV
LIDGPKAGVPRQAINLGQVVLTPLTFALPRGARTATVSKKWAAAGVCEKWAASSWAKK
IAQRERRAALTDFERFQVMVLRKQKRYTVKKALAKA"
centromere complement(105586..105703)
/note="CEN8; Chromosome VIII centromere"
/db_xref="SGD:S000006469"
centromere complement(105586..105610)
/note="CEN8_CDEIII of CEN8"
centromere complement(105611..105693)
/note="CEN8_CDEII of CEN8"
centromere complement(105694..105703)
/note="CEN8_CDEI of CEN8"
gene <106055..>107368
/gene="OSH7"
/locus_tag="YHR001W"
/db_xref="GeneID:856389"
mRNA <106055..>107368
/gene="OSH7"
/locus_tag="YHR001W"
/product="oxysterol-binding protein related protein OSH7"
/transcript_id="NM_001179131.1"
/db_xref="GeneID:856389"
CDS 106055..107368
/gene="OSH7"
/locus_tag="YHR001W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:16096648]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0008289 lipid
binding [PMID:20008566]"
/experiment="EXISTENCE:direct assay:GO:0032541 cortical
endoplasmic reticulum [PMID:20008566]"
/experiment="EXISTENCE:direct assay:GO:0120015 sterol
transfer activity [PMID:29487131]"
/experiment="EXISTENCE:genetic interaction:GO:0006887
exocytosis [PMID:17004323]"
/experiment="EXISTENCE:genetic interaction:GO:0006897
endocytosis [PMID:15173322]"
/experiment="EXISTENCE:genetic interaction:GO:0015918
sterol transport [PMID:16585271]"
/experiment="EXISTENCE:genetic interaction:GO:0016125
sterol metabolic process [PMID:16096648]"
/experiment="EXISTENCE:genetic interaction:GO:0030011
maintenance of cell polarity [PMID:17004323]"
/experiment="EXISTENCE:genetic interaction:GO:0034727
piecemeal microautophagy of the nucleus [PMID:15367582]"
/experiment="EXISTENCE:mutant phenotype:GO:0045324 late
endosome to vacuole transport [PMID:16096648]"
/note="Oxysterol-binding phosphatidylserine transferase;
localizes to ER-PM contact sites where it functions in
phosphatidylserine exchange from the ER to the PM driven
by PI4P; part of a seven-member family with overlapping,
redundant functions in sterol metabolism, and collectively
essential for viability; OSH7 has a paralog, OSH6, that
arose from the whole genome duplication"
/codon_start=1
/product="oxysterol-binding protein related protein OSH7"
/protein_id="NP_011863.1"
/db_xref="GeneID:856389"
/db_xref="SGD:S000001043"
/translation="MALNKLKNIPSLTNSSHSSINGIASNAANSKPSGADTDDIDEND
ESGQSILLNIISQLKPGCDLSRITLPTFILEKKSMLERITNQLQFPDVLLEAHSNKDG
LQRFVKVVAWYLAGWHIGPRAVKKPLNPILGEHFTAYWDLPNKQQAFYIAEQTSHHPP
ESAYFYMIPESNIRVDGVVVPKSKFLGNSSAAMMEGLTVLQFLDIKDANGKPEKYTLS
QPNVYARGILFGKMRIELGDHMVIMGPKYQVDIEFKTKGFISGTYDAIEGTIKDYDGK
EYYQISGKWNDIMYIKDLREKSSKKTVLFDTHQHFPLAPKVRPLEEQGEYESRRLWKK
VTDALAVRDHEVATEEKFQIENRQRELAKKRAEDGVEFHSKLFRRAEPGEDLDYYIYK
HIPEGTDKHEEQIRSILETAPILPGQTFTEKFSIPAYKKHGIQKN"
gene <107826..>108122
/gene="QCR10"
/locus_tag="YHR001W-A"
/db_xref="GeneID:856390"
mRNA join(<107826..107831,107895..>108122)
/gene="QCR10"
/locus_tag="YHR001W-A"
/product="ubiquinol--cytochrome-c reductase subunit 10"
/transcript_id="NM_001181426.1"
/db_xref="GeneID:856390"
CDS join(107826..107831,107895..108122)
/gene="QCR10"
/locus_tag="YHR001W-A"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:16823961|PMID:14576278|PMID:24769239|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0045275 respiratory
chain complex III [PMID:8175712]"
/experiment="EXISTENCE:genetic interaction:GO:0009060
aerobic respiration [PMID:8175712]"
/experiment="EXISTENCE:mutant phenotype:GO:0006122
mitochondrial electron transport, ubiquinol to cytochrome
c [PMID:8175712]"
/experiment="EXISTENCE:mutant phenotype:GO:0008121
quinol-cytochrome-c reductase activity [PMID:8175712]"
/note="Subunit of the ubiqunol-cytochrome c oxidoreductase
complex; this complex comprises part of the mitochondrial
respiratory chain; members include Cobp, Rip1p, Cyt1p,
Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p and
comprises part of the mitochondrial respiratory chain"
/codon_start=1
/product="ubiquinol--cytochrome-c reductase subunit 10"
/protein_id="NP_011864.1"
/db_xref="GeneID:856390"
/db_xref="SGD:S000003529"
/translation="MAYTSHLSSKTGLHFGRLSLRSLTAYAPNLMLWGGASMLGLFVF
TEGWPKFQDTLYKKIPLLGPTLEDHTPPEDKPN"
gene <108812..>109885
/gene="LEU5"
/locus_tag="YHR002W"
/db_xref="GeneID:856391"
mRNA <108812..>109885
/gene="LEU5"
/locus_tag="YHR002W"
/product="coenzyme A transporter"
/transcript_id="NM_001179132.1"
/db_xref="GeneID:856391"
CDS 108812..109885
/gene="LEU5"
/locus_tag="YHR002W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:11158296]"
/experiment="EXISTENCE:mutant phenotype:GO:0015228
coenzyme A transmembrane transporter activity
[PMID:11158296]"
/experiment="EXISTENCE:mutant phenotype:GO:0015880
coenzyme A transport [PMID:11158296]"
/note="Mitochondrial carrier protein; involved in the
accumulation of CoA in the mitochondrial matrix; homolog
of human Graves disease protein SLC25A16, which
complements yeast null mutant; does not encode an isozyme
of Leu4p, as first hypothesized"
/codon_start=1
/product="coenzyme A transporter"
/protein_id="NP_011865.1"
/db_xref="GeneID:856391"
/db_xref="SGD:S000001044"
/translation="MTRDSPDSNDSYKHINKNTTQKTSFDRNSFDYIVRSGLAGGISG
SCAKTLIAPLDRIKILFQTSNPHYTKYTGSLIGLVEAAKHIWINDGVRGFFQGHSATL
LRIFPYAAVKFVAYEQIRNTLIPSKEFESHWRRLVSGSLAGLCSVFITYPLDLVRVRL
AYETEHKRVKLGRIIKKIYKEPASATLIKNDYIPNWFCHWCNFYRGYVPTVLGMIPYA
GVSFFAHDLLHDVLKSPFFAPYSVLELSEDDELERVQKKQRRPLRTWAELISGGLAGM
ASQTAAYPFEIIRRRLQVSALSPKTMYDHKFQSISEIAHIIFKERGVRGFFVGLSIGY
IKVTPMVACSFFVYERMKWNFGI"
gene complement(<110028..>111317)
/gene="TCD1"
/locus_tag="YHR003C"
/db_xref="GeneID:856392"
mRNA complement(<110028..>111317)
/gene="TCD1"
/locus_tag="YHR003C"
/product="tRNA threonylcarbamoyladenosine dehydratase"
/transcript_id="NM_001179133.1"
/db_xref="GeneID:856392"
CDS complement(110028..111317)
/gene="TCD1"
/locus_tag="YHR003C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:16823961|PMID:14576278|PMID:24769239|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:16407407]"
/experiment="EXISTENCE:mutant phenotype:GO:0061503 tRNA
threonylcarbamoyladenosine dehydratase activity
[PMID:23242255]"
/experiment="EXISTENCE:mutant phenotype:GO:0061504 cyclic
threonylcarbamoyladenosine biosynthetic process
[PMID:23242255]"
/note="tRNA threonylcarbamoyladenosine dehydratase;
required for the ct6A tRNA base modification, where an
adenosine at position 37 is modified to form a cyclized
active ester with an oxazolone ring; localized to the
mitochondrial outer membrane; TCD1 has a paralog, TCD2,
that arose from the whole genome duplication"
/codon_start=1
/product="tRNA threonylcarbamoyladenosine dehydratase"
/protein_id="NP_011866.1"
/db_xref="GeneID:856392"
/db_xref="SGD:S000001045"
/translation="MANNTWKLIATTALISVFSTQLAKSVWKEYKLSCAANKNKTVSR
PRQYDDHLFREQLARNYAFLGEEGMRKIKEQYIVIVGAGEVGSWVCTMLIRSGCQKIM
IIDPENISIDSLNTHCCAVLSDIGKPKVQCLKEHLSKIAPWSEIKARAKAWTKENSHD
LIFADGESPTFIVDCLDNLESKVDLLEYAHHNKIDVISSMGVATKSDPTRVSINDISM
TEFDPISRCVRRKLRKRGIATGISVVFSNEMLDPRRDDILSPIDCEHRAINAVRDEAL
RHLPELGTMPGIFGLSIATWILTKVSGYPMKENEVKNRLKFYDSILETFQKQMARLNE
NKERSSLLGLEEVGYIVEEMFRGKSPISGYSTKLALTKWEANKEISLTNVVLMTKEEQ
EIHEKRILLDGEKLTAVYSEEVLDFIERLFKEEEYYS"
gene complement(<111754..>113094)
/gene="NEM1"
/locus_tag="YHR004C"
/db_xref="GeneID:856393"
mRNA complement(<111754..>113094)
/gene="NEM1"
/locus_tag="YHR004C"
/product="Nem1-Spo7 phosphatase catalytic subunit NEM1"
/transcript_id="NM_001179134.1"
/db_xref="GeneID:856393"
CDS complement(111754..113094)
/gene="NEM1"
/locus_tag="YHR004C"
/EC_number="3.1.3.16"
/experiment="EXISTENCE:direct assay:GO:0004721
phosphoprotein phosphatase activity
[PMID:25359770|PMID:15889145]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:32349126]"
/experiment="EXISTENCE:direct assay:GO:0005811 lipid
droplet [PMID:26275961]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:9822591]"
/experiment="EXISTENCE:genetic interaction:GO:0071072
negative regulation of phospholipid biosynthetic process
[PMID:15889145]"
/experiment="EXISTENCE:mutant phenotype:GO:0006998 nuclear
envelope organization [PMID:9822591]"
/experiment="EXISTENCE:mutant phenotype:GO:0046889
positive regulation of lipid biosynthetic process
[PMID:32349126]"
/experiment="EXISTENCE:mutant phenotype:GO:0071071
regulation of phospholipid biosynthetic process
[PMID:30201607]"
/experiment="EXISTENCE:mutant phenotype:GO:0140042 lipid
droplet formation [PMID:32349126]"
/experiment="EXISTENCE:physical interaction:GO:0071595
Nem1-Spo7 phosphatase complex [PMID:9822591]"
/note="Probable catalytic subunit of Nem1p-Spo7p
phosphatase holoenzyme; regulates nuclear/ER membrane
association and activation of Pah1p, a phosphatidate
phosphatase involved in the production of diacylglycerol
DAG for lipid droplet biogenesis; regulates nuclear growth
by controlling phospholipid biosynthesis; required for
normal nuclear envelope morphology and sporulation;
phosphatase activity of the Nem1p-Spo7p complex is
inhibited by Ice2p; homolog of the human protein Dullard"
/codon_start=1
/product="Nem1-Spo7 phosphatase catalytic subunit NEM1"
/protein_id="NP_011867.1"
/db_xref="GeneID:856393"
/db_xref="SGD:S000001046"
/translation="MNALKYFSNHLITTKKQKKINVEVTKNQDLLGPSKEVSNKYTSH
SENDCVSEVDQQYDHSSSHLKESDQNQERKNSVPKKPKALRSILIEKIASILWALLLF
LPYYLIIKPLMSLWFVFTFPLSVIERRVKHTDKRNRGSNASENELPVSSSNINDSSEK
TNPKNCNLNTIPEAVEDDLNASDEIILQRDNVKGSLLRAQSVKSRPRSYSKSELSLSN
HSSSNTVFGTKRMGRFLFPKKLIPKSVLNTQKKKKLVIDLDETLIHSASRSTTHSNSS
QGHLVEVKFGLSGIRTLYFIHKRPYCDLFLTKVSKWYDLIIFTASMKEYADPVIDWLE
SSFPSSFSKRYYRSDCVLRDGVGYIKDLSIVKDSEENGKGSSSSLDDVIIIDNSPVSY
AMNVDNAIQVEGWISDPTDTDLLNLLPFLEAMRYSTDVRNILALKHGEKAFNIN"
gene complement(<113499..>114917)
/gene="GPA1"
/locus_tag="YHR005C"
/gene_synonym="CDC70; DAC1; SCG1"
/db_xref="GeneID:856394"
mRNA complement(<113499..>114917)
/gene="GPA1"
/locus_tag="YHR005C"
/gene_synonym="CDC70; DAC1; SCG1"
/product="guanine nucleotide-binding protein subunit
alpha"
/transcript_id="NM_001179135.1"
/db_xref="GeneID:856394"
CDS complement(113499..114917)
/gene="GPA1"
/locus_tag="YHR005C"
/gene_synonym="CDC70; DAC1; SCG1"
/experiment="EXISTENCE:direct assay:GO:0003924 GTPase
activity [PMID:11394869]"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:16839886]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005834
heterotrimeric G-protein complex [PMID:11394869]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:8756677]"
/experiment="EXISTENCE:genetic interaction:GO:0000750
pheromone-dependent signal transduction involved in
conjugation with cellular fusion [PMID:2107073]"
/experiment="EXISTENCE:mutant phenotype:GO:0000742
karyogamy involved in conjugation with cellular fusion
[PMID:19386762]"
/experiment="EXISTENCE:mutant phenotype:GO:0000743 nuclear
migration involved in conjugation with cellular fusion
[PMID:19386762]"
/experiment="EXISTENCE:mutant phenotype:GO:0000750
pheromone-dependent signal transduction involved in
conjugation with cellular fusion [PMID:3113739]"
/experiment="EXISTENCE:mutant phenotype:GO:0003924 GTPase
activity [PMID:1900495]"
/experiment="EXISTENCE:mutant phenotype:GO:0005834
heterotrimeric G-protein complex [PMID:2105453]"
/experiment="EXISTENCE:mutant phenotype:GO:0006109
regulation of carbohydrate metabolic process
[PMID:34214075]"
/experiment="EXISTENCE:physical interaction:GO:0005834
heterotrimeric G-protein complex [PMID:11394869]"
/experiment="EXISTENCE:physical interaction:GO:0031681
G-protein beta-subunit binding [PMID:8417317]"
/note="G protein alpha subunit involved in the pheromone
response; GTP-binding subunit of the heterotrimeric G
protein; negatively regulates mating pathway by
sequestering G(beta)gamma and triggering an adaptive
response; interacts with the Ste5p scaffold protein to
enhance the efficiency of polarity site alignment;
activates Vps34p at endosome; protein abundance increases
in response to DNA replication stress; mutations in human
homolog GNAS associated with McCune-Albright syndrome"
/codon_start=1
/product="guanine nucleotide-binding protein subunit
alpha"
/protein_id="NP_011868.1"
/db_xref="GeneID:856394"
/db_xref="SGD:S000001047"
/translation="MGCTVSTQTIGDESDPFLQNKRANDVIEQSLQLEKQRDKNEIKL
LLLGAGESGKSTVLKQLKLLHQGGFSHQERLQYAQVIWADAIQSMKILIIQARKLGIQ
LDCDDPINNKDLFACKRILLKAKALDYINASVAGGSDFLNDYVLKYSERYETRRRVQS
TGRAKAAFDEDGNISNVKSDTDRDAETVTQNEDADRNNSSRINLQDICKDLNQEGDDQ
MFVRKTSREIQGQNRRNLIHEDIAKAIKQLWNNDKGIKQCFARSNEFQLEGSAAYYFD
NIEKFASPNYVCTDEDILKGRIKTTGITETEFNIGSSKFKVLDAGGQRSERKKWIHCF
EGITAVLFVLAMSEYDQMLFEDERVNRMHESIMLFDTLLNSKWFKDTPFILFLNKIDL
FEEKVKSMPIRKYFPDYQGRVGDAEAGLKYFEKIFLSLNKTNKPIYVKRTCATDTQTM
KFVLSAVTDLIIQQNLKKIGII"
gene complement(<115620..>115901)
/gene="TIM10"
/locus_tag="YHR005C-A"
/gene_synonym="MRS11"
/db_xref="GeneID:856395"
mRNA complement(<115620..>115901)
/gene="TIM10"
/locus_tag="YHR005C-A"
/gene_synonym="MRS11"
/product="protein transporter TIM10"
/transcript_id="NM_001181427.1"
/db_xref="GeneID:856395"
CDS complement(115620..115901)
/gene="TIM10"
/locus_tag="YHR005C-A"
/gene_synonym="MRS11"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0042719
mitochondrial intermembrane space chaperone complex
[PMID:9822593|PMID:9889188]"
/experiment="EXISTENCE:direct assay:GO:0045039 protein
insertion into mitochondrial inner membrane
[PMID:9495346]"
/experiment="EXISTENCE:direct assay:GO:0051082 unfolded
protein binding [PMID:12138093]"
/experiment="EXISTENCE:direct assay:GO:0140318 protein
transporter activity [PMID:9495346]"
/experiment="EXISTENCE:mutant phenotype:GO:0045039 protein
insertion into mitochondrial inner membrane
[PMID:9430585]"
/experiment="EXISTENCE:mutant phenotype:GO:0140318 protein
transporter activity [PMID:9430585]"
/note="Essential protein of the mitochondrial
intermembrane space; forms a complex with Tim9p (TIM10
complex) that delivers hydrophobic proteins to the TIM22
complex for insertion into the inner membrane"
/codon_start=1
/product="protein transporter TIM10"
/protein_id="NP_011869.1"
/db_xref="GeneID:856395"
/db_xref="SGD:S000003530"
/translation="MSFLGFGGGQPQLSSQQKIQAAEAELDLVTDMFNKLVNNCYKKC
INTSYSEGELNKNESSCLDRCVAKYFETNVQVGENMQKMGQSFNAAGKF"
gene complement(116107..116179)
/locus_tag="YNCH0003C"
/db_xref="GeneID:856396"
tRNA complement(116107..116179)
/locus_tag="YNCH0003C"
/product="tRNA-Thr"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:856396"
/db_xref="SGD:S000006740"
rep_origin 116179..116421
/note="ARS806; Putative replication origin; identified in
multiple array studies, not yet confirmed by plasmid-based
assay"
/db_xref="SGD:S000130146"
repeat_region complement(116421..116752)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006992"
repeat_region complement(116763..117061)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006993"
gene <117814..>119439
/gene="STP2"
/locus_tag="YHR006W"
/db_xref="GeneID:856397"
mRNA <117814..>119439
/gene="STP2"
/locus_tag="YHR006W"
/product="Stp2p"
/transcript_id="NM_001179136.1"
/db_xref="GeneID:856397"
CDS 117814..119439
/gene="STP2"
/locus_tag="YHR006W"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:11212916]"
/experiment="EXISTENCE:direct assay:GO:0001228 DNA-binding
transcription activator activity, RNA polymerase
II-specific [PMID:10648791|PMID:19906648]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:21127045]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:21127045]"
/experiment="EXISTENCE:mutant phenotype:GO:0001228
DNA-binding transcription activator activity, RNA
polymerase II-specific [PMID:10648791|PMID:19906648]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:10648791]"
/note="Transcription factor; activated by proteolytic
processing in response to signals from the SPS sensor
system for external amino acids; activates transcription
of amino acid permease genes; STP2 has a paralog, STP1,
that arose from the whole genome duplication"
/codon_start=1
/product="Stp2p"
/protein_id="NP_011870.1"
/db_xref="GeneID:856397"
/db_xref="SGD:S000001048"
/translation="MPILSLSSTRNSVLTRIYDYLKALVQQVIVPNVEDDKSSKSTPF
EKLEPAKQNHPQKDCCATEKDDLVDVSELFPKQNNKQLSLTSKSSVVPCALNLDNLET
PFSIKIDNNGAVTTQLNLDEPILRGPSRGEPAKLQNDLISSPPLEESYINNDQYKALF
PSNFLPITPVSSVITPASKKSIDESPLSDEVQGIADESSETLPYICHYCDARFRIRGY
LTRHIKKHAKRKAYHCPFFDNSISQELRCHTSGGFSRRDTYKTHLKSRHFTYPEGVKP
QDRNKSPGVCTQCGEHFSTSESWVENHIEAGSCKGLPEGYSEGIREKKKTSKMKMIKT
SDGQTRFISSDESVSEPALQNKNCIEATVMQSKERPNDKIIPTKTEKNDFGIGTQWFE
RKQISRPTQTTQSRGPTEVQNLKEWSIISPPILSPQNASSVPQEYQSSRYTLHMDSPA
LSSASSALSPLSGDPITTTETNKSYPLDSEQSLLEPDKTEEDAINQSKESNMISINEM
LQKQMDFELLGENHLKETQDYLALYKKAYGIEF"
gene complement(<120091..>121683)
/gene="ERG11"
/locus_tag="YHR007C"
/gene_synonym="CYP51"
/db_xref="GeneID:856398"
mRNA complement(<120091..>121683)
/gene="ERG11"
/locus_tag="YHR007C"
/gene_synonym="CYP51"
/product="sterol 14-demethylase"
/transcript_id="NM_001179137.1"
/db_xref="GeneID:856398"
CDS complement(120091..121683)
/gene="ERG11"
/locus_tag="YHR007C"
/gene_synonym="CYP51"
/EC_number="1.14.14.154"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:11914276|PMID:15951236|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0006696 ergosterol
biosynthetic process [PMID:105731]"
/experiment="EXISTENCE:direct assay:GO:0008398 sterol
14-demethylase activity [PMID:105731|PMID:9087488]"
/experiment="EXISTENCE:mutant phenotype:GO:0006696
ergosterol biosynthetic process [PMID:200835]"
/note="Lanosterol 14-alpha-demethylase; catalyzes C-14
demethylation of lanosterol to form 4,4''-dimethyl
cholesta-8,14,24-triene-3-beta-ol in ergosterol
biosynthesis pathway; transcriptionally down-regulated
when ergosterol is in excess; member of cytochrome P450
family; associated and coordinately regulated with the
P450 reductase Ncp1p; human CYP51A1 functionally
complements the lethality of the erg11 null mutation"
/codon_start=1
/product="sterol 14-demethylase"
/protein_id="NP_011871.1"
/db_xref="GeneID:856398"
/db_xref="SGD:S000001049"
/translation="MSATKSIVGEALEYVNIGLSHFLALPLAQRISLIIIIPFIYNIV
WQLLYSLRKDRPPLVFYWIPWVGSAVVYGMKPYEFFEECQKKYGDIFSFVLLGRVMTV
YLGPKGHEFVFNAKLADVSAEAAYAHLTTPVFGKGVIYDCPNSRLMEQKKFVKGALTK
EAFKSYVPLIAEEVYKYFRDSKNFRLNERTTGTIDVMVTQPEMTIFTASRSLLGKEMR
AKLDTDFAYLYSDLDKGFTPINFVFPNLPLEHYRKRDHAQKAISGTYMSLIKERRKNN
DIQDRDLIDSLMKNSTYKDGVKMTDQEIANLLIGVLMGGQHTSAATSAWILLHLAERP
DVQQELYEEQMRVLDGGKKELTYDLLQEMPLLNQTIKETLRMHHPLHSLFRKVMKDMH
VPNTSYVIPAGYHVLVSPGYTHLRDEYFPNAHQFNIHRWNKDSASSYSVGEEVDYGFG
AISKGVSSPYLPFGGGRHRCIGEHFAYCQLGVLMSIFIRTLKWHYPEGKTVPPPDFTS
MVTLPTGPAKIIWEKRNPEQKI"
gene complement(<122550..>122765)
/locus_tag="YHR007C-A"
/db_xref="GeneID:1466526"
mRNA complement(<122550..>122765)
/locus_tag="YHR007C-A"
/product="uncharacterized protein"
/transcript_id="NM_001184651.1"
/db_xref="GeneID:1466526"
CDS complement(122550..122765)
/locus_tag="YHR007C-A"
/note="hypothetical protein; identified by expression
profiling and mass spectrometry; SWAT-GFP fusion protein
localizes to the nucleus"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878086.1"
/db_xref="GeneID:1466526"
/db_xref="SGD:S000028830"
/translation="MHRKKRKKEKKRTEKDNTTNLPPLFLFPCSLSLPTLLAPVHYIP
TRLTHHQAENQLFLLLFQPIIVKPLRS"
gene complement(<122889..>123590)
/gene="SOD2"
/locus_tag="YHR008C"
/db_xref="GeneID:856399"
mRNA complement(<122889..>123590)
/gene="SOD2"
/locus_tag="YHR008C"
/product="superoxide dismutase SOD2"
/transcript_id="NM_001179138.1"
/db_xref="GeneID:856399"
CDS complement(122889..123590)
/gene="SOD2"
/locus_tag="YHR008C"
/EC_number="1.15.1.1"
/experiment="EXISTENCE:direct assay:GO:0004784 superoxide
dismutase activity [PMID:238997|PMID:15851472]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:15851472|PMID:24769239|PMID:14576278|PMID:11914276|P
MID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:238997]"
/experiment="EXISTENCE:mutant phenotype:GO:0072593
reactive oxygen species metabolic process [PMID:3520557]"
/note="Mitochondrial manganese superoxide dismutase;
protects cells against oxygen toxicity and oxidative
stress; human mitochondrial SOD2 can complement a yeast
null mutant and human cytoplasmic SOD1 can also complement
when targeted to the mitochondrial matrix"
/codon_start=1
/product="superoxide dismutase SOD2"
/protein_id="NP_011872.1"
/db_xref="GeneID:856399"
/db_xref="SGD:S000001050"
/translation="MFAKTAAANLTKKGGLSLLSTTARRTKVTLPDLKWDFGALEPYI
SGQINELHYTKHHQTYVNGFNTAVDQFQELSDLLAKEPSPANARKMIAIQQNIKFHGG
GFTNHCLFWENLAPESQGGGEPPTGALAKAIDEQFGSLDELIKLTNTKLAGVQGSGWA
FIVKNLSNGGKLDVVQTYNQDTVTGPLVPLVAIDAWEHAYYLQYQNKKADYFKAIWNV
VNWKEASRRFDAGKI"
gene complement(<124109..>125680)
/gene="TDA3"
/locus_tag="YHR009C"
/gene_synonym="BTN3"
/db_xref="GeneID:856400"
mRNA complement(<124109..>125680)
/gene="TDA3"
/locus_tag="YHR009C"
/gene_synonym="BTN3"
/product="Tda3p"
/transcript_id="NM_001179139.1"
/db_xref="GeneID:856400"
CDS complement(124109..125680)
/gene="TDA3"
/locus_tag="YHR009C"
/gene_synonym="BTN3"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:21441304|PMID:10684247]"
/experiment="EXISTENCE:direct assay:GO:0005770 late
endosome [PMID:21441304]"
/experiment="EXISTENCE:genetic interaction:GO:0042147
retrograde transport, endosome to Golgi [PMID:21441304]"
/experiment="EXISTENCE:mutant phenotype:GO:0042147
retrograde transport, endosome to Golgi [PMID:21441304]"
/experiment="EXISTENCE:physical interaction:GO:0042147
retrograde transport, endosome to Golgi [PMID:21441304]"
/note="Putative oxidoreductase involved in late endosome
to Golgi transport; physical and genetical interactions
with Btn2p; null mutant is viable, has extended S phase,
and sensitive to expression of top1-T722A allele; similar
to human FOXRED1"
/codon_start=1
/product="Tda3p"
/protein_id="NP_011873.1"
/db_xref="GeneID:856400"
/db_xref="SGD:S000001051"
/translation="MGEDFMHPPFQTYPSKNSEGKKHIVIVGGGIIGCCTAYYLTQHP
SFSPSTHHITIIESRRIAGGASGKAGGLLASWAFPHQIVPLSFQLHQELSDEYDGENN
WDYRRLTTVSLEADVREEVIENYERLSKKAYNLNVPPPKKRPGYISNKFNIGDSNSSL
SSSGSSLKNDSASNEEEGSDIHVSSSVPSLHSLTNERMRSHTNSASDLDSVSPVEQLR
ETNIHNPLPADLDWIRRELVNDWSSLGGTDTTAQLHPYKFTHFILSKAMETGAVDLLL
GKVVGLKCDEMDCVHSLKYLPSVVKNRRNSRGHAENPDIKLGTIFNDENAKPIEINDI
QQIVLSMGPWTSKILKDCPISGLRAHSVTIKPSEKTVSPYAILAELKVNDREFFSPEM
YARKDEVYVCGEGDTLVNIPESSDDVEVVSEKCDELYHYVSKLSPTLSKGHLLRKQAC
FLPVLNVPTSSGPLIGETNVKDLYIASGHSCWGINNAPATGKLMAEILLDGEATSAEI
SSLDPKLYFDATILS"
gene <126521..>127492
/gene="RPL27A"
/locus_tag="YHR010W"
/gene_synonym="RPL27"
/db_xref="GeneID:856401"
mRNA join(<126521..126551,127113..>127492)
/gene="RPL27A"
/locus_tag="YHR010W"
/gene_synonym="RPL27"
/product="60S ribosomal protein eL27 RPL27A"
/transcript_id="NM_001179140.1"
/db_xref="GeneID:856401"
CDS join(126521..126551,127113..127492)
/gene="RPL27A"
/locus_tag="YHR010W"
/gene_synonym="RPL27"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:11983894]"
/experiment="EXISTENCE:curator inference:GO:0003735
structural constituent of ribosome [PMID:11983894]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:11983894]"
/note="Ribosomal 60S subunit protein L27A; homologous to
mammalian ribosomal protein L27, no bacterial homolog;
RPL27A has a paralog, RPL27B, that arose from the whole
genome duplication"
/codon_start=1
/product="60S ribosomal protein eL27 RPL27A"
/protein_id="NP_011874.1"
/db_xref="GeneID:856401"
/db_xref="SGD:S000001052"
/translation="MAKFLKAGKVAVVVRGRYAGKKVVIVKPHDEGSKSHPFGHALVA
GIERYPLKVTKKHGAKKVAKRTKIKPFIKVVNYNHLLPTRYTLDVEAFKSVVSTETFE
QPSQREEAKKVVKKAFEERHQAGKNQWFFSKLRF"
gene <127780..>129120
/gene="DIA4"
/locus_tag="YHR011W"
/db_xref="GeneID:856402"
mRNA <127780..>129120
/gene="DIA4"
/locus_tag="YHR011W"
/product="putative serine--tRNA ligase DIA4"
/transcript_id="NM_001179141.1"
/db_xref="GeneID:856402"
CDS 127780..129120
/gene="DIA4"
/locus_tag="YHR011W"
/EC_number="6.1.1.11"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239]"
/experiment="EXISTENCE:genetic interaction:GO:0004828
serine-tRNA ligase activity [PMID:11747308]"
/experiment="EXISTENCE:genetic interaction:GO:0070158
mitochondrial seryl-tRNA aminoacylation [PMID:11747308]"
/experiment="EXISTENCE:mutant phenotype:GO:0005739
mitochondrion [PMID:11747308]"
/note="Probable mitochondrial seryl-tRNA synthetase;
mutant displays increased invasive and pseudohyphal
growth"
/codon_start=1
/product="putative serine--tRNA ligase DIA4"
/protein_id="NP_011875.1"
/db_xref="GeneID:856402"
/db_xref="SGD:S000001053"
/translation="MIIRRLFSISNRSFFLKKPQFDVKKIIEMIPQYQTSIQNRELIE
ADSIIRSLQLLGERYQNIKEIDKVIADIQIQRKSIEAQIKKDKTKITEYSAALKALKE
QYNDQNSKSSELKKKILETCKSLPNTLDPTVPLDAPQIEQWINPLKTHKTSEAQAHVD
IMLKKNMLDLQTASNVTGMSWYYLLNDGARLEQALVAYALKKANENGFSSCVPPSITK
KELIDACGFNPRDMNNERQIYALQDTNLGLVATAEIPLAGLGANKVLELNSGECSKKL
VGVSRCYRAEAGARGKDTKGLYRVHEFTKVELFCWSKPETSAKVLEEIKQFQISVVEE
LGIPAKVLNMPSNDLGNPAFKKYDIEAWMPGRGKFGEISSASNCTDFQSRRLNTKYRD
DNTGKLEYVHTLNGTAMAIPRVIVALVENFYDPSTGKISVPECLREFMNGQRYI"
gene <129481..>130448
/gene="VPS29"
/locus_tag="YHR012W"
/gene_synonym="PEP11; VPT6"
/db_xref="GeneID:856403"
mRNA join(<129481..129528,129648..>130448)
/gene="VPS29"
/locus_tag="YHR012W"
/gene_synonym="PEP11; VPT6"
/product="retromer subunit VPS29"
/transcript_id="NM_001179142.1"
/db_xref="GeneID:856403"
CDS join(129481..129528,129648..130448)
/gene="VPS29"
/locus_tag="YHR012W"
/gene_synonym="PEP11; VPT6"
/experiment="EXISTENCE:direct assay:GO:0170071 CROP
complex [PMID:35466426]"
/experiment="EXISTENCE:mutant phenotype:GO:0030904
retromer complex [PMID:9700157]"
/experiment="EXISTENCE:mutant phenotype:GO:0140312 cargo
adaptor activity [PMID:9700157]"
/experiment="EXISTENCE:physical interaction:GO:0005768
endosome [PMID:9700157]"
/experiment="EXISTENCE:physical interaction:GO:0030904
retromer complex [PMID:9700157]"
/experiment="EXISTENCE:physical interaction:GO:0030906
retromer, cargo-selective complex [PMID:9700157]"
/experiment="EXISTENCE:physical interaction:GO:0042147
retrograde transport, endosome to Golgi [PMID:9700157]"
/experiment="EXISTENCE:physical interaction:GO:0140312
cargo adaptor activity [PMID:9700157]"
/note="Subunit of the membrane-associated retromer
complex; endosomal protein; essential for
endosome-to-Golgi retrograde transport; forms a subcomplex
with Vps35p and Vps26p that selects cargo proteins for
endosome-to-Golgi retrieval"
/codon_start=1
/product="retromer subunit VPS29"
/protein_id="NP_011876.1"
/db_xref="GeneID:856403"
/db_xref="SGD:S000001054"
/translation="MLLLALSDAHIPDRATDLPVKFKKLLSVPDKISQVALLGNSTKS
YDFLKFVNQISNNITIVRGEFDNGHLPSTKKDKASDNSRPMEEIPMNSIIRQGALKIG
CCSGYTVVPKNDPLSLLALARQLDVDILLWGGTHNVEAYTLEGKFFVNPGSCTGAFNT
DWPIVFDVEDSDEAVTSEVDKPTKENQSEDDDAKGGSTGKEQPGSYTPKEGTAGEREN
ENESNVKPENQFKEDEVDMSDSDINGSNSPSFCLLDIQGNTCTLYIYLYVNGEVKVDK
VVYEKE"
gene complement(<130730..>131446)
/gene="ARD1"
/locus_tag="YHR013C"
/gene_synonym="NAA10"
/db_xref="GeneID:856404"
mRNA complement(<130730..>131446)
/gene="ARD1"
/locus_tag="YHR013C"
/gene_synonym="NAA10"
/product="peptide alpha-N-acetyltransferase complex A
subunit ARD1"
/transcript_id="NM_001179143.1"
/db_xref="GeneID:856404"
CDS complement(130730..131446)
/gene="ARD1"
/locus_tag="YHR013C"
/gene_synonym="NAA10"
/EC_number="2.3.1.255"
/experiment="EXISTENCE:direct assay:GO:0004596
protein-N-terminal amino-acid acetyltransferase activity
[PMID:2551674]"
/experiment="EXISTENCE:direct assay:GO:0006474 N-terminal
protein amino acid acetylation [PMID:2551674]"
/experiment="EXISTENCE:direct assay:GO:0031415 NatA
complex [PMID:14517307]"
/experiment="EXISTENCE:mutant phenotype:GO:0004596
protein-N-terminal amino-acid acetyltransferase activity
[PMID:2551674]"
/experiment="EXISTENCE:mutant phenotype:GO:0006474
N-terminal protein amino acid acetylation [PMID:2551674]"
/experiment="EXISTENCE:mutant phenotype:GO:0061606
N-terminal protein amino acid propionylation
[PMID:23043182]"
/note="Subunit of protein N-terminal acetyltransferase
NatA; NatA comprises Nat1p, Ard1p, Nat5p; acetylates many
proteins to influence telomeric silencing, cell cycle,
heat-shock resistance, mating, sporulation, early stages
of mitophagy; protein abundance increases under DNA
replication stress; mutations in human homolog X-linked
NAA10 lead to Ogden syndrome (S37P) and intellectual
disability (R116W); expression of human NAA10 and NAA15
can complement ard1 nat1 double mutant"
/codon_start=1
/product="peptide alpha-N-acetyltransferase complex A
subunit ARD1"
/protein_id="NP_011877.1"
/db_xref="GeneID:856404"
/db_xref="SGD:S000001055"
/translation="MPINIRRATINDIICMQNANLHNLPENYMMKYYMYHILSWPEAS
FVATTTTLDCEDSDEQDENDKLELTLDGTNDGRTIKLDPTYLAPGEKLVGYVLVKMND
DPDQQNEPPNGHITSLSVMRTYRRMGIAENLMRQALFALREVHQAEYVSLHVRQSNRA
ALHLYRDTLAFEVLSIEKSYYQDGEDAYAMKKVLKLEELQISNFTHRRLKENEEKLED
DLESDLLEDIIKQGVNDIIV"
gene <132047..>132922
/gene="SPO13"
/locus_tag="YHR014W"
/db_xref="GeneID:856405"
mRNA <132047..>132922
/gene="SPO13"
/locus_tag="YHR014W"
/product="Spo13p"
/transcript_id="NM_001179144.1"
/db_xref="GeneID:856405"
CDS 132047..132922
/gene="SPO13"
/locus_tag="YHR014W"
/experiment="EXISTENCE:direct assay:GO:0000779 condensed
chromosome, centromeric region [PMID:15620645]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12101125]"
/experiment="EXISTENCE:direct assay:GO:0030295 protein
kinase activator activity [PMID:38413836]"
/experiment="EXISTENCE:direct assay:GO:0072687 meiotic
spindle [PMID:17493939]"
/experiment="EXISTENCE:mutant phenotype:GO:0045876
positive regulation of sister chromatid cohesion
[PMID:12101125]"
/experiment="EXISTENCE:mutant phenotype:GO:0051177 meiotic
sister chromatid cohesion [PMID:15620644|PMID:15620645]"
/experiment="EXISTENCE:mutant phenotype:GO:0071459 protein
localization to chromosome, centromeric region
[PMID:16357219]"
/note="Meiotic regulator; involved in maintaining sister
chromatid cohesion during meiosis I as well as promoting
proper attachment of kinetochores to the spindle during
meiosis I and meiosis II; promotes phosphorylation of
Cdc5p substrates in MI and impacts Hrr25p function in MII;
substrate of the anaphase-promoting complex (APC) that is
degraded during anaphase I; expressed solely in meiotic
cells"
/codon_start=1
/product="Spo13p"
/protein_id="NP_011878.1"
/db_xref="GeneID:856405"
/db_xref="SGD:S000001056"
/translation="MAPRKRFRLLELGSPTHSKRKVQKPLQEKTPNLRVSPLAFKIGK
EIKNKEIRKTKKTESENIFNSKHVDLRLESPHPGLNFVSDAQQYSKAGDVRYLKNKSS
NTLKNERQTIERPSFDNSLRFEDIEQPPKSTSTPVLSQSSQINVEREAPMFPVPYYIA
PSPMYNFSPYQNFVGNPTFLTPSHNPNLNYAIPIQRPELLYPNVNVYDSPLFKKTRLP
HQTKSLDKEKNYQYLPIYPVSISNNGDFVGQETPRAAPKLSKKRLSNTLDVNCSDYES
SGQNATYNDSESSLN"
gene complement(133026..133107)
/locus_tag="YNCH0004C"
/db_xref="GeneID:856406"
tRNA complement(133026..133107)
/locus_tag="YNCH0004C"
/product="tRNA-Ser"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0030371 translation
repressor activity [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043555 regulation
of translation in response to stress [PMID:27609601]"
/note="Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:856406"
/db_xref="SGD:S000006726"
repeat_region complement(133201..133442)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006994"
rep_origin 133438..133539
/note="ARS807; Autonomously Replicating Sequence"
/db_xref="SGD:S000118357"
repeat_region complement(133565..133684)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006995"
repeat_region 133685..134009
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007007"
gene 134321..134392
/locus_tag="YNCH0005W"
/db_xref="GeneID:856407"
tRNA 134321..134392
/locus_tag="YNCH0005W"
/product="tRNA-Gln"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:15706032]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:15706032]"
/note="Glutamine tRNA (tRNA-Gln), predicted by tRNAscan-SE
analysis; thiolation of uridine at wobble position (34)
requires Ncs6p"
/db_xref="GeneID:856407"
/db_xref="SGD:S000006698"
gene <134554..>136533
/gene="MIP6"
/locus_tag="YHR015W"
/db_xref="GeneID:856408"
mRNA <134554..>136533
/gene="MIP6"
/locus_tag="YHR015W"
/product="Mip6p"
/transcript_id="NM_001179145.1"
/db_xref="GeneID:856408"
CDS 134554..136533
/gene="MIP6"
/locus_tag="YHR015W"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:28193845]"
/experiment="EXISTENCE:genetic interaction:GO:0006417
regulation of translation [PMID:28193845]"
/experiment="EXISTENCE:genetic interaction:GO:0010609 mRNA
localization resulting in post-transcriptional regulation
of gene expression [PMID:28193845]"
/experiment="EXISTENCE:genetic interaction:GO:0016071 mRNA
metabolic process [PMID:28193845]"
/experiment="EXISTENCE:genetic interaction:GO:0043934
sporulation [PMID:28193845]"
/note="mRNA-binding protein; interacts with the UBA domain
of mRNA export factor Mex67p; participates in the export
of Msn2/4 stress-dependent mRNAs; partially redundant
function with paralog PES4 in the steady-state expression,
translational timing and localization of a subset of
Ndt80-dependent mRNAs that are protected until translated
near the end of meiosis II; redundant role with PES4 in
sporulation; shuttles between the nucleus and cytoplasm,
localizes to stress granules and the prospore membrane"
/codon_start=1
/product="Mip6p"
/protein_id="NP_011879.1"
/db_xref="GeneID:856408"
/db_xref="SGD:S000001057"
/translation="MPNSHGNVLNNISLNSKQNPRSISKSCPNDKDARQKSFKTISAQ
ALVRVQGAGYKLGDVKLKDAEVKEKNSLKKYDCKNATQEKKEQEQVFEKTVAKGSVQK
YITKTSKTNSLFIGNLKSTVTEEMLRKIFKRYQSFESAKVCRDFLTKKSLGYGYLNFK
DKNDAESARKEFNYTVFFGQEVKIMPSMKNTLFRKNIGTNVFFSNLPLENPQLTTRSF
YLIMIEYGNVLSCLLERRKNIGFVYFDNDISARNVIKKYNNQEFFGNKIICGLHFDKE
VRTRPEFTKRKKMIGSDIVIEDELLASNNLSDNARSKTILVKNLPSDTTQEEVLDYFS
TIGPIKSVFISEKQANTPHKAFVTYKNEEESKKAQKCLNKTIFKNHTIWVGPGKDKPV
HNQIGTNKKTKVYLKNLSFNCNKEFISQLCLQEKIRFSEIKITNYNSLNWTFCGHVEC
FSRSDAERLFNILDRRLIGSSLVEASWSKNNDNILNEIDYDDGNNNENYKKLINISSM
MRFRTQELSAHQKGLTSQFQQVVSPFSSYSNSYTNMNSLVATPMKPHPAFNLITNTVD
EKLHQPKRTKQENAEILESLKKIINRNLQRISISGLNKEENLRSISEFIFDVFWEHDS
ERLSHFLLMTNTSLESQKILQKQVTRAAESLGFTV"
gene complement(<136881..>138455)
/gene="YSC84"
/locus_tag="YHR016C"
/gene_synonym="LSB4"
/db_xref="GeneID:856409"
mRNA complement(join(<136881..138240,138409..>138455))
/gene="YSC84"
/locus_tag="YHR016C"
/gene_synonym="LSB4"
/product="Ysc84p"
/transcript_id="NM_001179146.1"
/db_xref="GeneID:856409"
CDS complement(join(136881..138240,138409..138455))
/gene="YSC84"
/locus_tag="YHR016C"
/gene_synonym="LSB4"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0030479 actin
cortical patch [PMID:12388763]"
/experiment="EXISTENCE:direct assay:GO:0051015 actin
filament binding [PMID:19158382]"
/experiment="EXISTENCE:direct assay:GO:0051017 actin
filament bundle assembly [PMID:19158382]"
/experiment="EXISTENCE:genetic interaction:GO:0006897
endocytosis [PMID:12388763]"
/experiment="EXISTENCE:genetic interaction:GO:0030036
actin cytoskeleton organization [PMID:12388763]"
/experiment="EXISTENCE:genetic interaction:GO:0051666
actin cortical patch localization [PMID:19158382]"
/experiment="EXISTENCE:mutant phenotype:GO:0051666 actin
cortical patch localization [PMID:19158382]"
/note="Actin-binding protein; involved in bundling of
actin filaments and endocytosis of actin cortical patches;
activity stimulated by Las17p; contains SH3 domain similar
to Rvs167p; YSC84 has a paralog, LSB3, that arose from the
whole genome duplication"
/codon_start=1
/product="Ysc84p"
/protein_id="NP_011880.1"
/db_xref="GeneID:856409"
/db_xref="SGD:S000001058"
/translation="MGINNPIPRSLKSETKKAAKVLRSFVKPNQVFGADQVIPPYVLK
RAKGLAIITVLKAGFLFSGRAGSGVIVARLKDGTWSAPSAIAMAGAGAGGMVGVELTD
FVFILNSEEAVRSFSEFGTITLGGNVSVSAGPLGRSAEAAASASTGGVSAVFAYSKSK
GLFAGVSVEGSAILERREANRKFYGDNCTSKMILSGRVKVPPAADPLLRILESRAFNF
TRHDHDDNASGDDFYDDGQYSDNTSHYYDDIPDSFDSTDESSTRPNTRSSRRRGMSLG
SRSRYDDDYDDDGYGRGRGYGDFDSEDEDYDYGRSPNRNSSRNRGPQIDRGTKPRANT
RWEDDLYDRDTEYSRPNHSGRDYDNTRGNRRGYGRERGYSLGHGPTHPSNMSNVDDLS
HKMSKTGLGNESTATNSATPTAVALYNFAGEQPGDLAFKKGDVITILKKSDSQNDWWT
GRTNGKEGIFPANYVRVS"
gene <138694..>139851
/gene="YSC83"
/locus_tag="YHR017W"
/db_xref="GeneID:856410"
mRNA <138694..>139851
/gene="YSC83"
/locus_tag="YHR017W"
/product="Ysc83p"
/transcript_id="NM_001179147.1"
/db_xref="GeneID:856410"
CDS 138694..139851
/gene="YSC83"
/locus_tag="YHR017W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:16407407]"
/note="Non-essential mitochondrial hypothetical protein;
mRNA induced during meiosis, peaking between mid to late
prophase of meiosis I; similar to S. douglasii YSD83"
/codon_start=1
/product="Ysc83p"
/protein_id="NP_011881.1"
/db_xref="GeneID:856410"
/db_xref="SGD:S000001059"
/translation="MAWSGGKDIVDQIFDAGYWLVSKSAVLSDEIKNHVEKSIESISG
KISNKETPRLQENDSNRSKVYKTLRIGLQDHWKLGLGISATSLCLYLGYRTFFKLPPN
LPEAESQVVLILGDMNDPIIRNQVMDLYRRRFTVYICTENADVYKKHEEDQDFIYYID
PTCEKDFEGFFVDVPRLASILFMPRLSYHPSGVISCDSLESEIHSSIFVYYQALLSII
PHLKRKTQLIMFNPSLTADLNLVHHSTEIITSGIIDSLFKIFKEYQRLNVSTIKLGIL
QIGSQPSNYKFLRMAGSDIHEALHYPVYKMIMSANGYKLRQLLSWLTTLGGYNSVYYC
GRFSYLVSWPFASLIFNHHTRLSLKRLRGRLAKVYSSIISFFCRSSSKSSK"
gene complement(<140011..>141402)
/gene="ARG4"
/locus_tag="YHR018C"
/db_xref="GeneID:856411"
mRNA complement(<140011..>141402)
/gene="ARG4"
/locus_tag="YHR018C"
/product="argininosuccinate lyase ARG4"
/transcript_id="NM_001179148.1"
/db_xref="GeneID:856411"
CDS complement(140011..141402)
/gene="ARG4"
/locus_tag="YHR018C"
/EC_number="4.3.2.1"
/experiment="EXISTENCE:direct assay:GO:0004056
argininosuccinate lyase activity [PMID:4578972]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:205532]"
/experiment="EXISTENCE:direct assay:GO:0042450 L-arginine
biosynthetic process via ornithine [PMID:4578972]"
/note="Argininosuccinate lyase; catalyzes the final step
in the arginine biosynthesis pathway"
/codon_start=1
/product="argininosuccinate lyase ARG4"
/protein_id="NP_011882.1"
/db_xref="GeneID:856411"
/db_xref="SGD:S000001060"
/translation="MSDGTQKLWGGRFTGETDPLMHLYNASLPYDYKMYKADLEGTKV
YTAGLQKLGLLTETELAKIHEGLAEIKKEWDADKFVRHPNDEDIHTANERRLGELIGR
DIAGKVHTGRSRNDQVVTDLRIYCRDIVNDTLFPALKGLVEVLIKRAEGEIDVLMPGY
THLQRAQPIRWSHWLSSYATYFTEDYKRLGQILHRLNQSPLGAGALAGHPYGIDREFL
AEGLGFNSVIGNSLVAVSDRDFIVELMFWGTLFMNHISRFAEDLIIYCTAEFGFIQLS
DAYSTGSSLMPQKKNADSLELLRGKSGRVFGDLTGFLMSLKGIPSTYDKDMQEDKEPL
FDCLTTVEHSMLIATGVISTLTVNKEKMEAALTMDMLATDLADYLVRKGVPFRETHHI
SGECVATAERLGLSGIDKLTLEQYQKIDSRFGQDLFETFNFEQSVERRDATGGTAKSA
VLKQLDNLKSQLN"
rep_origin 140349..141274
/note="ARS2; Autonomously Replicating Sequence"
/db_xref="SGD:S000029042"
gene complement(<141894..>143558)
/gene="DED81"
/locus_tag="YHR019C"
/db_xref="GeneID:856412"
mRNA complement(<141894..>143558)
/gene="DED81"
/locus_tag="YHR019C"
/product="asparagine--tRNA ligase DED81"
/transcript_id="NM_001179149.1"
/db_xref="GeneID:856412"
CDS complement(141894..143558)
/gene="DED81"
/locus_tag="YHR019C"
/EC_number="6.1.1.22"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9605503]"
/experiment="EXISTENCE:direct assay:GO:0004816
asparagine-tRNA ligase activity [PMID:9605503]"
/experiment="EXISTENCE:direct assay:GO:0006421
asparaginyl-tRNA aminoacylation [PMID:9605503]"
/experiment="EXISTENCE:direct assay:GO:1990825
sequence-specific mRNA binding [PMID:34039240]"
/note="Cytosolic asparaginyl-tRNA synthetase; required for
protein synthesis, catalyzes the specific attachment of
asparagine to its cognate tRNA"
/codon_start=1
/product="asparagine--tRNA ligase DED81"
/protein_id="NP_011883.1"
/db_xref="GeneID:856412"
/db_xref="SGD:S000001061"
/translation="MSSLYIKEATGVDELTTAGSQDHPFKTPAYALFASQQKSDATEP
KLFVFKTEDNEYQEISASALKKARKGCDGLKKKAVKQKEQELKKQQKEAENAAKQLSA
LNITIKEDESLPAAIKTRIYDSYSKVGQRVKVSGWIHRLRSNKKVIFVVLRDGSGFIQ
CVLSGDLALAQQTLDLTLESTVTLYGTIVKLPEGKTAPGGVELNVDYYEVVGLAPGGE
DSFTNKIAEGSDPSLLLDQRHLALRGDALSAVMKVRAALLKSVRRVYDEEHLTEVTPP
CMVQTQVEGGSTLFKMNYYGEEAYLTQSSQLYLETCLASLGDVYTIQESFRAEKSHTR
RHLSEYTHIEAELAFLTFDDLLQHIETLIVKSVQYVLEDPIAGPLVKQLNPNFKAPKA
PFMRLQYKDAITWLNEHDIKNEEGEDFKFGDDIAEAAERKMTDTIGVPIFLTRFPVEI
KSFYMKRCSDDPRVTESVDVLMPNVGEITGGSMRIDDMDELMAGFKREGIDTDAYYWF
IDQRKYGTCPHGGYGIGTERILAWLCDRFTVRDCSLYPRFSGRCKP"
gene <143996..>146062
/locus_tag="YHR020W"
/db_xref="GeneID:856413"
mRNA <143996..>146062
/locus_tag="YHR020W"
/product="proline--tRNA ligase"
/transcript_id="NM_001179150.1"
/db_xref="GeneID:856413"
CDS 143996..146062
/locus_tag="YHR020W"
/EC_number="6.1.1.15"
/experiment="EXISTENCE:direct assay:GO:0004827
proline-tRNA ligase activity [PMID:16864571]"
/experiment="EXISTENCE:direct assay:GO:0006433 prolyl-tRNA
aminoacylation [PMID:16864571]"
/note="Prolyl-tRNA synthetase; N-terminal domain shows
weak homology to prokaryotic posttransfer editing domain,
but does not possess posttransfer editing activity; may
interact with ribosomes, based on co-purification
experiments"
/codon_start=1
/product="proline--tRNA ligase"
/protein_id="NP_011884.1"
/db_xref="GeneID:856413"
/db_xref="SGD:S000001062"
/translation="MPVSEAFAKLCVNEKPPAESAVAVKSLVFKPKTPKSATPVPIVV
VALQSTTTPSALIANATSSKDPRLARDDLVKQAFQSESARAFILGDLANATSNFHLLI
DHELGTVDGDTILQLNDSTYMKKSDMMKFLNNFEDSQKVVDFSQEVSKETATEGKKQQ
KKQQPSKAGTAAAAAAAALEDAKLIGITVDKALDFPGWYQQILTKGEMLDYYDVSGCY
ILRPPSYAIWENIQKWFDDKIKAIGVQNAYFPMFVSSRVLEKEKDHVEGFAPEVAWVT
RAGSSELEEPIAIRPTSETVMYPYYAKWVQSYRDLPLKLNQWNSVVRWEFKHPQPFLR
TREFLWQEGHTAHLTAKDAEEEVLQILDFYAGVYEELLAVPVVKGRKTEKEKFAGGDF
TTTCEGYIPQTGRGIQGATSHHLGQNFSKMFNLSVENPLGSDHPKIFAYQNSWGLSTR
VIGVMVMIHSDNKGLVIPPRVSQFQSVVIPVGITKKTSEEQRKHIHETARSVESRLKK
VGIRAFGDYNDNYTPGWKFSQYELKGIPIRIELGPKDIEKNQVVVVRRNDSKKYVVSF
DELEARIPEILEEMQGDLFKKAKELFDTHRVIVNEWSGFVPALNKKNVILAPWCGVME
CEEDIKESSAKKDDGEEFEEDDKAPSMGAKSLCIPFDQPVLNEGQKCIKCERIAVNYC
MFGRSY"
gene complement(146242..146314)
/locus_tag="YNCH0006C"
/db_xref="GeneID:856414"
tRNA complement(146242..146314)
/locus_tag="YNCH0006C"
/product="tRNA-Ala"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE
analysis; one of 11 nuclear tRNA genes containing the
tDNA-anticodon AGC (converted to IGC in the mature tRNA),
decodes GCU and GCC codons into alanine, one of 16 nuclear
tRNAs for alanine"
/db_xref="GeneID:856414"
/db_xref="SGD:S000006513"
repeat_region complement(146331..146671)
/note="Ty3 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007003"
repeat_region 146844..147114
/note="Ty4 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007010"
gene complement(<147871..>148669)
/gene="RPS27B"
/locus_tag="YHR021C"
/db_xref="GeneID:856415"
mRNA complement(join(<147871..148116,148667..>148669))
/gene="RPS27B"
/locus_tag="YHR021C"
/product="40S ribosomal protein eS27 RPS27B"
/transcript_id="NM_001179151.1"
/db_xref="GeneID:856415"
CDS complement(join(147871..148116,148667..148669))
/gene="RPS27B"
/locus_tag="YHR021C"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:3533916]"
/experiment="EXISTENCE:direct assay:GO:0022627 cytosolic
small ribosomal subunit [PMID:3533916]"
/experiment="EXISTENCE:genetic interaction:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16246728]"
/experiment="EXISTENCE:mutant phenotype:GO:0000028
ribosomal small subunit assembly [PMID:9271380]"
/experiment="EXISTENCE:mutant phenotype:GO:0000479
endonucleolytic cleavage of tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:9271380]"
/note="Protein component of the small (40S) ribosomal
subunit; homologous to mammalian ribosomal protein S27, no
bacterial homolog; RPS27B has a paralog, RPS27A, that
arose from the whole genome duplication"
/codon_start=1
/product="40S ribosomal protein eS27 RPS27B"
/protein_id="NP_011885.1"
/db_xref="GeneID:856415"
/db_xref="SGD:S000001063"
/translation="MVLVQDLLHPTAASEARKHKLKTLVQGPRSYFLDVKCPGCLNIT
TVFSHAQTAVTCESCSTVLCTPTGGKAKLSEGTSFRRK"
gene <149225..>149680
/gene="ECM12"
/locus_tag="YHR021W-A"
/db_xref="GeneID:856416"
mRNA <149225..>149680
/gene="ECM12"
/locus_tag="YHR021W-A"
/product="Ecm12p"
/transcript_id="NM_001181428.1"
/db_xref="GeneID:856416"
CDS 149225..149680
/gene="ECM12"
/locus_tag="YHR021W-A"
/note="hypothetical protein; may contribute to cell wall
biosynthesis, mutants display zymolyase hypersensitivity"
/codon_start=1
/product="Ecm12p"
/protein_id="NP_011886.1"
/db_xref="GeneID:856416"
/db_xref="SGD:S000003531"
/translation="MNRSFHFLIKYIYIHVLLVFYFHIKQQAIMPFFIFFFSSFDGLS
FDLRVVAFLAKHVFVGVCSPFFVVGFFGSSRVVVTEWLSKLVLPPPPVSITQVFSLSR
KRGEFSSGYILIINPYKSFLRSLLDFSIFNNTAKNKSSTFTLNLEDVSK"
gene complement(<149575..>150345)
/locus_tag="YHR022C"
/db_xref="GeneID:856417"
mRNA complement(<149575..>150345)
/locus_tag="YHR022C"
/product="uncharacterized protein"
/transcript_id="NM_001179152.1"
/db_xref="GeneID:856417"
CDS complement(149575..150345)
/locus_tag="YHR022C"
/note="hypothetical protein; YHR022C is not an essential
gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011887.1"
/db_xref="GeneID:856417"
/db_xref="SGD:S000001064"
/translation="MSVRLSYGASLASIPRCFDLKSSKITVMGDDHSGKTSLVRSWLG
SSFQISDANRYRVSDLYHKTIQFDTLVKYYRTFGVKGQLPNYAGFKAKNSGTIYESCG
NFLEERLINANKSTAQRRTSIDVQVFDTNQMEVSYLSELTTLQIRQSDAIILCFDSTN
DSSLASLESYICIIHHVRLECELDIPIIIACTKCDLDSERTITHEKVLTFIQELGFSP
GNLDYFETSSKFNVNVEDLFLAVLLKIEKSKSDRRKLL"
gene complement(<151217..>151306)
/locus_tag="YHR022C-A"
/db_xref="GeneID:1466527"
mRNA complement(<151217..>151306)
/locus_tag="YHR022C-A"
/product="uncharacterized protein"
/transcript_id="NM_001184597.1"
/db_xref="GeneID:1466527"
CDS complement(151217..151306)
/locus_tag="YHR022C-A"
/note="hypothetical protein; identified by gene-trapping,
microarray-based expression analysis, and genome-wide
homology searching"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878087.1"
/db_xref="GeneID:1466527"
/db_xref="SGD:S000028645"
/translation="MKIKFSRGARFSATFSFDKYPFLLYEVVR"
gene <151666..>157452
/gene="MYO1"
/locus_tag="YHR023W"
/db_xref="GeneID:856418"
mRNA <151666..>157452
/gene="MYO1"
/locus_tag="YHR023W"
/product="myosin 1"
/transcript_id="NM_001179153.1"
/db_xref="GeneID:856418"
CDS 151666..157452
/gene="MYO1"
/locus_tag="YHR023W"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:9732290]"
/experiment="EXISTENCE:direct assay:GO:0000142 cellular
bud neck contractile ring [PMID:9442111]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:9732290]"
/experiment="EXISTENCE:direct assay:GO:1904498 protein
localization to mitotic actomyosin contractile ring
[PMID:15210731]"
/experiment="EXISTENCE:mutant phenotype:GO:0000281 mitotic
cytokinesis [PMID:21173112]"
/experiment="EXISTENCE:mutant phenotype:GO:0000920 septum
digestion after cytokinesis [PMID:9732290]"
/experiment="EXISTENCE:mutant phenotype:GO:0031671 primary
cell septum biogenesis [PMID:21173112]"
/experiment="EXISTENCE:mutant phenotype:GO:1902404 mitotic
actomyosin contractile ring contraction
[PMID:21173112|PMID:9864366|PMID:16148042]"
/experiment="EXISTENCE:mutant phenotype:GO:1903475 mitotic
actomyosin contractile ring assembly
[PMID:9732290|PMID:21173112]"
/experiment="EXISTENCE:mutant phenotype:GO:1904498 protein
localization to mitotic actomyosin contractile ring
[PMID:23468521]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding
[PMID:11082046|PMID:21173112|PMID:23468521]"
/experiment="EXISTENCE:physical interaction:GO:0016460
myosin II complex [PMID:15210731]"
/experiment="EXISTENCE:physical interaction:GO:0032027
myosin light chain binding [PMID:15210731]"
/experiment="EXISTENCE:physical interaction:GO:0032033
myosin II light chain binding [PMID:11082046]"
/note="Type II myosin heavy chain; required for wild-type
cytokinesis and cell separation; localizes to the
actomyosin ring; binds to myosin light chains Mlc1p and
Mlc2p through its IQ1 and IQ2 motifs respectively"
/codon_start=1
/product="myosin 1"
/protein_id="NP_011888.1"
/db_xref="GeneID:856418"
/db_xref="SGD:S000001065"
/translation="MTGGQSCSSNMIVWIPDEKEVFVKGELMSTDINKNKFTGQEEQI
GIVHPLDSTEVSNLVQVRISDVFPVNPSTFDKVENMSELTHLNEPSVLYNLEKRYDCD
LIYTYSGLFLVAINPYHNLNLYSEDHINLYHNKHNRLSKSRLDENSHEKLPPHIFAIA
EEAYENLLSEGKDQSILVTGESGAGKTENTKKILQYLASITSGSPSNIAPVSGSSIVE
SFEMKILQSNPILESFGNAQTVRNNNSSRFGKFIKIEFNEHGMINGAHIEWYLLEKSR
IVHQNSKERNYHIFYQLLSGLDDSELKNLRLKSRNVKDYKILSNSNQDIIPGINDVEN
FKELLSALNIIGFSKDQIRWIFQVVAIILLIGNIEFVSDRAEQASFKNDVSAICSNLG
VDEKDFQTAILRPRSKAGKEWVSQSKNSQQAKFILNALSRNLYERLFGYIVDMINKNL
DHGSATLNYIGLLDIAGFEIFENNSFEQLCINYTNEKLQQFFNNHMFVLEQSEYLKEN
IQWDYIDYGKDLQLTIDLIESKGPPTGVLPLLDEEAVLPKSTDESFYSKLISTWDQNS
SKFKRSRLKNGFILKHYAGDVEYTVEGWLSKNKDPLNDNLLSLLSSSQNDIISKLFQP
EGEKSSSAGVEANISNQEVKKSARTSTFKTTSSRHREQQITLLNQLASTHPHFVRCII
PNNVKKVKTFNRRLILDQLRCNGVLEGIRLAREGYPNRIAFQEFFQRYRILYPENSTT
TTFSSKLKASTKQNCEFLLTSLQLDTKVYKIGNTKLFFKAGVLADLEKQKDVKLNNIM
IKLTATIRGYTVRKEITYHLQKLKKTRVIGNTFRLYNRLVKEDPWFNLFIRIKPLLTS
SNDMTRTKKFNEQINKLKNDLQEMESKKKFLEEKNQKTVNELENTQDLLNQEKENLRK
NESLLNRVKTSSETLQKQFDDLVSEKDEISREKLEVAQNLEEAHQKIQGLQETIRERE
ATLEKLHSKNNELIKQISDLNCDISKEQSSQSLIKESKLKLENEIKRLKDVINSKEEE
IKSFNDKLSSSEEDLDIKLVTLEKNCNIAMSRLQSLVTENSDLRSKNENFKKEKAALN
NQLKNKESELLKMKEKIDNHKKELATFSKQRDDAVSEHGKITAELKETRIQLTEYKSN
YQKIKEEYSNFQRETKEQEQKKRNSLVESLNDSKIKELEARLSQEISLNQYLNKRISG
NSVETNISSTRRSTSYSDDPLDKEDIIKKYYDLQLAFTEITRNLENEIEEKKNLISRL
RFTETRLASSSFEDQKIKAQMKKLKKLIQDMDPSIPLDSILNEPLDNCPDKESDINKL
MLEVDYLKRQLDIETRAHYDAENAISALHSKFRKIQGESSLSSSDIYKLKFEASEERV
KSLEDKLKTMPLRDRTNLPVGDIIKNRDSISKYEEEIRYYKLENYKLQEILNESNGKL
SQLTLDLRQSKSKEALLSEQLDRLQKDLESTERQKELLSSTIKQQKQQFENCMDDLQG
NELRLREHIHALKQAEEDVKNMASIIEKLKTQNKQKEKLIWEREMERNDSDMQLQETL
LELKRVQDVKKILSDDLAHLKERLSAVEDRSQYTDEINRLKEELNCSLKAETNLKKEF
ATLKYKLETSTNDSEAKISDLLKQLDHYTKVVEMLNNEKDAISLAEKELYQKYEALNT
ECESLKGKIVSLTKIKQELESDLNQKTDALQISNAALSSSTQKNKEITEKIKYLEETL
QLQMEQNSRNGELVKTLQASCNGYKDKFDDEKQKNIDLYEENQTLQKLNTDLQLQLKN
LHERLSDTTEKNAWLSKIHELENMVSLETDLKYEEMKKNKSLERAVEELQTKNSQQTD
VIELANKNRSEFEEATLKYEAQISDLEKYISQQELEMKKSIRDNSSYRDKVQEMAQEI
EFWKSRYESTMIGSKNIDSNNAQSKIFS"
gene complement(<157744..>159192)
/gene="MAS2"
/locus_tag="YHR024C"
/gene_synonym="MIF2"
/db_xref="GeneID:856419"
mRNA complement(<157744..>159192)
/gene="MAS2"
/locus_tag="YHR024C"
/gene_synonym="MIF2"
/product="mitochondrial-processing protease subunit alpha"
/transcript_id="NM_001179154.1"
/db_xref="GeneID:856419"
CDS complement(157744..159192)
/gene="MAS2"
/locus_tag="YHR024C"
/gene_synonym="MIF2"
/experiment="EXISTENCE:direct assay:GO:0004222
metalloendopeptidase activity [PMID:2905264]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0009003 signal
peptidase activity [PMID:2007593]"
/experiment="EXISTENCE:direct assay:GO:0017087
mitochondrial processing peptidase complex [PMID:2905264]"
/experiment="EXISTENCE:mutant phenotype:GO:0006627 protein
processing involved in protein targeting to mitochondrion
[PMID:3061808]"
/note="Alpha subunit of the mitochondrial processing
protease (MPP); essential processing enzyme that cleaves
the N-terminal targeting sequences from mitochondrially
imported proteins"
/codon_start=1
/product="mitochondrial-processing protease subunit alpha"
/protein_id="NP_011889.1"
/db_xref="GeneID:856419"
/db_xref="SGD:S000001066"
/translation="MLRNGVQRLYSNIARTDNFKLSSLANGLKVATSNTPGHFSALGL
YIDAGSRFEGRNLKGCTHILDRLAFKSTEHVEGRAMAETLELLGGNYQCTSSRENLMY
QASVFNQDVGKMLQLMSETVRFPKITEQELQEQKLSAEYEIDEVWMKPELVLPELLHT
AAYSGETLGSPLICPRELIPSISKYYLLDYRNKFYTPENTVAAFVGVPHEKALELTEK
YLGDWQSTHPPITKKVAQYTGGESCIPPAPVFGNLPELFHIQIGFEGLPIDHPDIYAL
ATLQTLLGGGGSFSAGGPGKGMYSRLYTHVLNQYYFVENCVAFNHSYSDSGIFGISLS
CIPQAAPQAVEVIAQQMYNTFANKDLRLTEDEVSRAKNQLKSSLLMNLESKLVELEDM
GRQVLMHGRKIPVNEMISKIEDLKPDDISRVAEMIFTGNVNNAGNGKGRATVVMQGDR
GSFGDVENVLKAYGLGNSSSSKNDSPKKKGWF"
gene <159438..>160511
/gene="THR1"
/locus_tag="YHR025W"
/db_xref="GeneID:856420"
mRNA <159438..>160511
/gene="THR1"
/locus_tag="YHR025W"
/product="homoserine kinase"
/transcript_id="NM_001179155.1"
/db_xref="GeneID:856420"
CDS 159438..160511
/gene="THR1"
/locus_tag="YHR025W"
/EC_number="2.7.1.39"
/experiment="EXISTENCE:direct assay:GO:0004413 homoserine
kinase activity [PMID:2165904]"
/experiment="EXISTENCE:direct assay:GO:0009092 homoserine
metabolic process [PMID:2165904]"
/experiment="EXISTENCE:mutant phenotype:GO:0009088
L-threonine biosynthetic process [PMID:17247984]"
/note="Homoserine kinase; conserved protein required for
threonine biosynthesis; long-lived protein that is
preferentially retained in mother cells and forms
cytoplasmic filaments; expression is regulated by the
GCN4-mediated general amino acid control pathway"
/codon_start=1
/product="homoserine kinase"
/protein_id="NP_011890.1"
/db_xref="GeneID:856420"
/db_xref="SGD:S000001067"
/translation="MVRAFKIKVPASSANIGPGYDVLGVGLSLFLELDVTIDSSQAQE
TNDDPNNCKLSYTKESEGYSTVPLRSDANLITRTALYVLRCNNIRNFPSGTKVHVSNP
IPLGRGLGSSGAAVVAGVILGNEVAQLGFSKQRMLDYCLMIERHPDNITAAMMGGFCG
SFLRDLTPQEVERREIPLAEVLPEPSGGEDTGLVPPLPPTDIGRHVKYQWNPAIKCIA
IIPQFELSTADSRGVLPKAYPTQDLVFNLQRLAVLTTALTMDPPNADLIYPAMQDRVH
QPYRKTLIPGLTEILSCVTPSTYPGLLGICLSGAGPTILALATENFEEISQEIINRFA
KNGIKCSWKLLEPAYDGASVEQQ"
gene <160844..>161485
/gene="VMA16"
/locus_tag="YHR026W"
/gene_synonym="PPA1"
/db_xref="GeneID:856421"
mRNA <160844..>161485
/gene="VMA16"
/locus_tag="YHR026W"
/gene_synonym="PPA1"
/product="H(+)-transporting V0 sector ATPase subunit c''"
/transcript_id="NM_001179156.1"
/db_xref="GeneID:856421"
CDS 160844..161485
/gene="VMA16"
/locus_tag="YHR026W"
/gene_synonym="PPA1"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:15252052]"
/experiment="EXISTENCE:mutant phenotype:GO:0007035
vacuolar acidification [PMID:9030535]"
/note="Subunit c'' of the vacuolar ATPase; v-ATPase
functions in acidification of the vacuole; one of three
proteolipid subunits of the V0 domain"
/codon_start=1
/product="H(+)-transporting V0 sector ATPase subunit c''"
/protein_id="NP_011891.1"
/db_xref="GeneID:856421"
/db_xref="SGD:S000001068"
/translation="MNKESKDDDMSLGKFSFSHFLYYLVLIVVIVYGLYKLFTGHGSD
INFGKFLLRTSPYMWANLGIALCVGLSVVGAAWGIFITGSSMIGAGVRAPRITTKNLI
SIIFCEVVAIYGLIIAIVFSSKLTVATAENMYSKSNLYTGYSLFWAGITVGASNLICG
IAVGITGATAAISDAADSALFVKILVIEIFGSILGLLGLIVGLLMAGKASEFQ"
gene complement(<161730..>164711)
/gene="RPN1"
/locus_tag="YHR027C"
/gene_synonym="HRD2; NAS1"
/db_xref="GeneID:856422"
mRNA complement(<161730..>164711)
/gene="RPN1"
/locus_tag="YHR027C"
/gene_synonym="HRD2; NAS1"
/product="proteasome regulatory particle base subunit
RPN1"
/transcript_id="NM_001179157.1"
/db_xref="GeneID:856422"
CDS complement(161730..164711)
/gene="RPN1"
/locus_tag="YHR027C"
/gene_synonym="HRD2; NAS1"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:26928762|PMID:15210724]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0008540 proteasome
regulatory particle, base subcomplex [PMID:9741626]"
/experiment="EXISTENCE:direct assay:GO:0034515 proteasome
storage granule [PMID:18504300]"
/experiment="EXISTENCE:physical interaction:GO:0030674
protein-macromolecule adaptor activity [PMID:12198498]"
/note="Non-ATPase base subunit of the 19S RP of the 26S
proteasome; may participate in the recognition of several
ligands of the proteasome; contains a leucine-rich repeat
(LRR) domain, a site for protein-protein interactions; RP
is the acronym for regulatory particle"
/codon_start=1
/product="proteasome regulatory particle base subunit
RPN1"
/protein_id="NP_011892.1"
/db_xref="GeneID:856422"
/db_xref="SGD:S000001069"
/translation="MVDESDKKQQTIDEQSQISPEKQTPNKKDKKKEEEEQLSEEDAK
LKTDLELLVERLKEDDSSLYEASLNALKESIKNSTSSMTAVPKPLKFLRPTYPDLCSI
YDKWTDPNLKSSLADVLSILAMTYSENGKHDSLRYRLLSDVSDFEGWGHEYIRHLALE
IGEVYNDQVEKDAEDETSSDGSKSDGSAATSGFEFSKEDTLRLCLDIVPYFLKHNGEE
DAVDLLLEIESIDKLPQFVDENTFQRVCQYMVACVPLLPPPEDVAFLKTAYSIYLSQN
ELTDAIALAVRLGEEDMIRSVFDATSDPVMHKQLAYILAAQKTSFEYEGVQDIIGNGK
LSEHFLYLAKELNLTGPKVPEDIYKSHLDNSKSVFSSAGLDSAQQNLASSFVNGFLNL
GYCNDKLIVDNDNWVYKTKGDGMTSAVASIGSIYQWNLDGLQQLDKYLYVDEPEVKAG
ALLGIGISASGVHDGEVEPALLLLQDYVTNPDTKISSAAILGLGIAFAGSKNDEVLGL
LLPIAASTDLPIETAAMASLALAHVFVGTCNGDITTSIMDNFLERTAIELKTDWVRFL
ALALGILYMGQGEQVDDVLETISAIEHPMTSAIEVLVGSCAYTGTGDVLLIQDLLHRL
TPKNVKGEEDADEEETAEGQTNSISDFLGEQVNEPTKNEEAEIEVDEMEVDAEGEEVE
VKAEITEKKNGESLEGEEIKSEEKKGKSSDKDATTDGKNDDEEEEKEAGIVDELAYAV
LGIALIALGEDIGKEMSLRHFGHLMHYGNEHIRRMVPLAMGIVSVSDPQMKVFDTLTR
FSHDADLEVSMNSIFAMGLCGAGTNNARLAQLLRQLASYYSREQDALFITRLAQGLLH
LGKGTMTMDVFNDAHVLNKVTLASILTTAVGLVSPSFMLKHHQLFYMLNAGIRPKFIL
ALNDEGEPIKVNVRVGQAVETVGQAGRPKKITGWITQSTPVLLNHGERAELETDEYIS
YTSHIEGVVILKKNPDYREEE"
gene complement(<164978..>167434)
/gene="DAP2"
/locus_tag="YHR028C"
/gene_synonym="DPP2"
/db_xref="GeneID:856423"
mRNA complement(<164978..>167434)
/gene="DAP2"
/locus_tag="YHR028C"
/gene_synonym="DPP2"
/product="dipeptidyl aminopeptidase"
/transcript_id="NM_001179158.1"
/db_xref="GeneID:856423"
CDS complement(164978..167434)
/gene="DAP2"
/locus_tag="YHR028C"
/gene_synonym="DPP2"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762|PMID:2647766]"
/experiment="EXISTENCE:direct assay:GO:0008239
dipeptidyl-peptidase activity [PMID:2647766]"
/note="Dipeptidyl aminopeptidase; synthesized as a
glycosylated precursor; localizes to the vacuolar
membrane; similar to Ste13p"
/codon_start=1
/product="dipeptidyl aminopeptidase"
/protein_id="NP_011893.1"
/db_xref="GeneID:856423"
/db_xref="SGD:S000001070"
/translation="MEGGEEEVERIPDELFDTKKKHLLDKLIRVGIILVLLIWGTVLL
LKSIPHHSNTPDYQEPNSNYTNDGKLKVSFSVVRNNTFQPKYHELQWISDNKIESNDL
GLYVTFMNDSYVVKSVYDDSYNSVLLEGKTFIHNGQNLTVESITASPDLKRLLIRTNS
VQNWRHSTFGSYFVYDKSSSSFEEIGNEVALAIWSPNSNDIAYVQDNNIYIYSAISKK
TIRAVTNDGSSFLFNGKPDWVYEEEVFEDDKAAWWSPTGDYLAFLKIDESEVGEFIIP
YYVQDEKDIYPEMRSIKYPKSGTPNPHAELWVYSMKDGTSFHPRISGNKKDGSLLITE
VTWVGNGNVLVKTTDRSSDILTVFLIDTIAKTSNVVRNESSNGGWWEITHNTLFIPAN
ETFDRPHNGYVDILPIGGYNHLAYFENSNSSHYKTLTEGKWEVVNGPLAFDSMENRLY
FISTRKSSTERHVYYIDLRSPNEIIEVTDTSEDGVYDVSFSSGRRFGLLTYKGPKVPY
QKIVDFHSRKAEKCDKGNVLGKSLYHLEKNEVLTKILEDYAVPRKSFRELNLGKDEFG
KDILVNSYEILPNDFDETLSDHYPVFFFAYGGPNSQQVVKTFSVGFNEVVASQLNAIV
VVVDGRGTGFKGQDFRSLVRDRLGDYEARDQISAASLYGSLTFVDPQKISLFGWSYGG
YLTLKTLEKDGGRHFKYGMSVAPVTDWRFYDSVYTERYMHTPQENFDGYVESSVHNVT
ALAQANRFLLMHGTGDDNVHFQNSLKFLDLLDLNGVENYDVHVFPDSDHSIRYHNANV
IVFDKLLDWAKRAFDGQFVK"
gene complement(<167677..>168561)
/gene="YHI9"
/locus_tag="YHR029C"
/db_xref="GeneID:856424"
mRNA complement(<167677..>168561)
/gene="YHI9"
/locus_tag="YHR029C"
/product="Yhi9p"
/transcript_id="NM_001179159.1"
/db_xref="GeneID:856424"
CDS complement(167677..168561)
/gene="YHI9"
/locus_tag="YHR029C"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:31323700]"
/experiment="EXISTENCE:mutant phenotype:GO:0030968
endoplasmic reticulum unfolded protein response
[PMID:16582425]"
/note="hypothetical protein; null mutant is defective in
unfolded protein response; possibly involved in a membrane
regulation metabolic pathway; member of the PhzF
superfamily, though most likely not involved in phenazine
production"
/codon_start=1
/product="Yhi9p"
/protein_id="NP_011894.1"
/db_xref="GeneID:856424"
/db_xref="SGD:S000001071"
/translation="MTLMVPFKQVDVFTEKPFMGNPVAVINFLEIDENEVSQEELQAI
ANWTNLSETTFLFKPSDKKYDYKLRIFTPRSELPFAGHPTIGSCKAFLEFTKNTTATS
LVQECKIGAVPITINEGLISFKAPMADYESISSEMIADYEKAIGLKFIKPPALLHTGP
EWIVALVEDAETCFNANPNFAMLAHQTKQNDHVGIILAGPKKEAAIKNSYEMRAFAPV
INVYEDPVCGSGSVALARYLQEVYKFEKTTDITISEGGRLKRNGLMLASIKKEADNST
SYYIAGHATTVIDGKIKV"
rep_origin 168549..168723
/note="ARS809; Autonomously Replicating Sequence"
/db_xref="SGD:S000118358"
gene complement(<168890..>170344)
/gene="SLT2"
/locus_tag="YHR030C"
/gene_synonym="BYC2; LYT2; MPK1; SLK2"
/db_xref="GeneID:856425"
mRNA complement(<168890..>170344)
/gene="SLT2"
/locus_tag="YHR030C"
/gene_synonym="BYC2; LYT2; MPK1; SLK2"
/product="mitogen-activated serine/threonine-protein
kinase SLT2"
/transcript_id="NM_001179160.1"
/db_xref="GeneID:856425"
CDS complement(168890..170344)
/gene="SLT2"
/locus_tag="YHR030C"
/gene_synonym="BYC2; LYT2; MPK1; SLK2"
/EC_number="2.7.11.24"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0004674 protein
serine/threonine kinase activity
[PMID:20823268|PMID:24554767]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:26928762|PMID:12361575|PMID:23924898]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:25598154]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:12361575|PMID:23924898]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:12361575|PMID:23924898]"
/experiment="EXISTENCE:direct assay:GO:0030968 endoplasmic
reticulum unfolded protein response [PMID:16380504]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:12361575]"
/experiment="EXISTENCE:genetic interaction:GO:0000196 cell
integrity MAPK cascade [PMID:8386320|PMID:8386319]"
/experiment="EXISTENCE:genetic interaction:GO:0009272
fungal-type cell wall biogenesis [PMID:16524906]"
/experiment="EXISTENCE:genetic interaction:GO:0010973
positive regulation of division septum assembly
[PMID:19633265|PMID:27400980]"
/experiment="EXISTENCE:genetic interaction:GO:0060237
regulation of fungal-type cell wall organization
[PMID:8386319]"
/experiment="EXISTENCE:mutant phenotype:GO:0000425
pexophagy [PMID:20385774]"
/experiment="EXISTENCE:mutant phenotype:GO:0007165 signal
transduction [PMID:8386319]"
/experiment="EXISTENCE:mutant phenotype:GO:0008361
regulation of cell size [PMID:17302939]"
/experiment="EXISTENCE:mutant phenotype:GO:0010447
response to acidic pH [PMID:16087742]"
/experiment="EXISTENCE:mutant phenotype:GO:0030968
endoplasmic reticulum unfolded protein response
[PMID:16380504]"
/experiment="EXISTENCE:mutant phenotype:GO:0034976
response to endoplasmic reticulum stress [PMID:27117666]"
/experiment="EXISTENCE:mutant phenotype:GO:0036498
IRE1-mediated unfolded protein response [PMID:18971375]"
/experiment="EXISTENCE:mutant phenotype:GO:0042306
regulation of protein import into nucleus [PMID:20219973]"
/experiment="EXISTENCE:mutant phenotype:GO:0060237
regulation of fungal-type cell wall organization
[PMID:8386319]"
/experiment="EXISTENCE:mutant phenotype:GO:0060256
regulation of flocculation [PMID:31441971]"
/experiment="EXISTENCE:mutant phenotype:GO:0090364
regulation of proteasome assembly [PMID:27462806]"
/note="Serine/threonine MAP kinase; coordinates expression
of all 19S regulatory particle assembly-chaperones (RACs)
to control proteasome abundance; involved in regulating
maintenance of cell wall integrity, cell cycle
progression, nuclear mRNA retention in heat shock, septum
assembly; required for mitophagy, pexophagy; affects
recruitment of mitochondria to phagophore assembly site;
plays role in adaptive response of cells to cold;
regulated by the PKC1-mediated signaling pathway"
/codon_start=1
/product="mitogen-activated serine/threonine-protein
kinase SLT2"
/protein_id="NP_011895.1"
/db_xref="GeneID:856425"
/db_xref="SGD:S000001072"
/translation="MADKIERHTFKVFNQDFSVDKRFQLIKEIGHGAYGIVCSARFAE
AAEDTTVAIKKVTNVFSKTLLCKRSLRELKLLRHFRGHKNITCLYDMDIVFYPDGSIN
GLYLYEELMECDMHQIIKSGQPLTDAHYQSFTYQILCGLKYIHSADVLHRDLKPGNLL
VNADCQLKICDFGLARGYSENPVENSQFLTEYVATRWYRAPEIMLSYQGYTKAIDVWS
AGCILAEFLGGKPIFKGKDYVNQLNQILQVLGTPPDETLRRIGSKNVQDYIHQLGFIP
KVPFVNLYPNANSQALDLLEQMLAFDPQKRITVDEALEHPYLSIWHDPADEPVCSEKF
EFSFESVNDMEDLKQMVIQEVQDFRLFVRQPLLEEQRQLQLQQQQQQQQQQQQQQQQP
SDVDNGNAAASEENYPKQMATSNSVAPQQESFGIHSQNLPRHDADFPPRPQESMMEMR
PATGNTADIPPQNDNGTLLDLEKELEFGLDRKYF"
gene complement(<170799..>172970)
/gene="RRM3"
/locus_tag="YHR031C"
/gene_synonym="RTT104"
/db_xref="GeneID:856426"
mRNA complement(<170799..>172970)
/gene="RRM3"
/locus_tag="YHR031C"
/gene_synonym="RTT104"
/product="DNA helicase"
/transcript_id="NM_001179161.1"
/db_xref="GeneID:856426"
CDS complement(170799..172970)
/gene="RRM3"
/locus_tag="YHR031C"
/gene_synonym="RTT104"
/EC_number="5.6.2.3"
/experiment="EXISTENCE:direct assay:GO:0003678 DNA
helicase activity [PMID:12050116]"
/experiment="EXISTENCE:direct assay:GO:0005657 replication
fork [PMID:26411679|PMID:17114583]"
/experiment="EXISTENCE:direct assay:GO:0019237 centromeric
DNA binding [PMID:30442759]"
/experiment="EXISTENCE:direct assay:GO:0043596 nuclear
replication fork [PMID:26981397]"
/experiment="EXISTENCE:genetic interaction:GO:0071932
replication fork reversal [PMID:26411679]"
/experiment="EXISTENCE:mutant phenotype:GO:0005657
replication fork [PMID:17114583]"
/experiment="EXISTENCE:mutant phenotype:GO:0006260 DNA
replication [PMID:10693764]"
/experiment="EXISTENCE:mutant phenotype:GO:0097046
replication fork progression beyond termination site
[PMID:20797631]"
/note="DNA helicase involved in rDNA replication and Ty1
transposition; binds to and suppresses DNA damage at G4
motifs in vivo; relieves replication fork pauses at
telomeric regions; deletion or inactivation of helicase
function suppresses chromosome instability (CIN)
associated with centromere or kinetochore defects;
structurally and functionally related to Pif1p"
/codon_start=1
/product="DNA helicase"
/protein_id="NP_011896.1"
/db_xref="GeneID:856426"
/db_xref="SGD:S000001073"
/translation="MFRSHASGNKKQWSKRSSNGSTPAASASGSHAYRQQTLSSFFMG
CGKKSAAASKNSTTIIDLESGDEGNRNITAPPRPRLIRNNSSSLFSQSQGSFGDDDPD
AEFKKLVDVPRLNSYKKSSRSLSMTSSLHKTASASTTQKTYHFDEDETLREVTSVKSN
SRQLSFTSTINIEDSSMKLSTDSERPAKRSKPSMEFQGLKLTVPKKIKPLLRKTVSNM
DSMNHRSASSPVVLTMEQERVVNLIVKKRTNVFYTGSAGTGKSVILQTIIRQLSSLYG
KESIAITASTGLAAVTIGGSTLHKWSGIGIGNKTIDQLVKKIQSQKDLLAAWRYTKVL
IIDEISMVDGNLLDKLEQIARRIRKNDDPFGGIQLVLTGDFFQLPPVAKKDEHNVVKF
CFESEMWKRCIQKTILLTKVFRQQDNKLIDILNAIRYGELTVDIAKTIRNLNRDIDYA
DGIAPTELYATRREVELSNVKKLQSLPGDLYEFKAVDNAPERYQAILDSSLMVEKVVA
LKEDAQVMMLKNKPDVELVNGSLGKVLFFVTESLVVKMKEIYKIVDDEVVMDMRLVSR
VIGNPLLKESKEFRQDLNARPLARLERLKILINYAVKISPHKEKFPYVRWTVGKNKYI
HELMVPERFPIDIPRENVGLERTQIPLMLCWALSIHKAQGQTIQRLKVDLRRIFEAGQ
VYVALSRAVTMDTLQVLNFDPGKIRTNERVKDFYKRLETLK"
gene <173344..>175089
/gene="ERC1"
/locus_tag="YHR032W"
/db_xref="GeneID:856427"
mRNA <173344..>175089
/gene="ERC1"
/locus_tag="YHR032W"
/product="Erc1p"
/transcript_id="NM_001179162.1"
/db_xref="GeneID:856427"
CDS 173344..175089
/gene="ERC1"
/locus_tag="YHR032W"
/experiment="EXISTENCE:mutant phenotype:GO:0006556
S-adenosylmethionine biosynthetic process [PMID:18592490]"
/note="Member of the multi-drug and toxin extrusion (MATE)
family; the MATE family is part of the
multidrug/oligosaccharidyl-lipid/polysaccharide (MOP)
exporter superfamily; overproduction confers ethionine
resistance and accumulation of S-adenosylmethionine"
/codon_start=1
/product="Erc1p"
/protein_id="NP_011897.1"
/db_xref="GeneID:856427"
/db_xref="SGD:S000001074"
/translation="MSKQFSHTTNDRRSSIIYSTSVGKAGLFTPADYIPQESEENLIE
GEEQEGSEEEPSYTGNDDETEREGEYHSLLDANNSRTLQQEAWQQGYDSHDRKRLLDE
ERDLLIDNKLLSQHGNGGGDIESHGHGQAIGPDEEERPAEIANTWESAIESGQKISTT
FKRETQVITMNALPLIFTFILQNSLSLASIFSVSHLGTKELGGVTLGSMTANITGLAA
IQGLCTCLDTLCAQAYGAKNYHLVGVLVQRCAVITILAFLPMMYVWFVWSEKILALMI
PERELCALAANYLRVTAFGVPGFILFECGKRFLQCQGIFHASTIVLFVCAPLNALMNY
LLVWNDKIGIGYLGAPLSVVINYWLMTLGLLIYAMTTKHKERPLKCWNGIIPKEQAFK
NWRKMINLAIPGVVMVEAEFLGFEVLTIFASHLGTDALGAQSIVATIASLAYQVPFSI
SVSTSTRVANFIGASLYDSCMITCRVSLLLSFVCSSMNMFVICRYKEQIASLFSTESA
VVKMVVDTLPLLAFMQLFDAFNASTAGCLRGQGRQKNRWVHQPSRILLPRCAHGICVS
IPVSSGCRRLMVGYN"
gene <175548..>176819
/locus_tag="YHR033W"
/db_xref="GeneID:856428"
mRNA <175548..>176819
/locus_tag="YHR033W"
/product="putative glutamate 5-kinase"
/transcript_id="NM_001179163.1"
/db_xref="GeneID:856428"
CDS 175548..176819
/locus_tag="YHR033W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/note="hypothetical protein; epitope-tagged protein
localizes to the cytoplasm; YHR033W has a paralog, PRO1,
that arose from the whole genome duplication"
/codon_start=1
/product="putative glutamate 5-kinase"
/protein_id="NP_011898.1"
/db_xref="GeneID:856428"
/db_xref="SGD:S000001075"
/translation="MTKAYTIVIKLGSSSLVDESTKEPKLSIMTLIVETVTNLKRMGH
KVIIVSSGGIAVGLDALNIPHKPKQLSEVQAIAAVGQGRLIARWNMLFSQYGEQTAQI
LLTRNDILRWNQYNNARNTINELLAMGVIPIVNENDTLSISEIEFGDNDTLSAITAAL
VGADFLFLLTDVDCLYTDNPRTNPDARPIVLVPELSEGLPGVNTSSGSGSEVGTGGMR
TKLIAADLASNAGIETIVMKSDRPEYVPKIVDYIQHHFRPPRHIGNGTQQQFLDLQDT
ELEQLRRYDVPMHTKFLANDNKHKLKNREFWILHGLITKGAIIIDENSYDKLLSKDMA
SLTPNAVIEVRDNFHELECVDLKIGQRLPNGELDISKPIQSVGCVRSNYTSLELAKIK
GLPSEKIHDVLGYSVSEYVAHRENIAFPPQF"
gene complement(<176965..>177999)
/gene="PIH1"
/locus_tag="YHR034C"
/gene_synonym="NOP17"
/db_xref="GeneID:856429"
mRNA complement(<176965..>177999)
/gene="PIH1"
/locus_tag="YHR034C"
/gene_synonym="NOP17"
/product="Pih1p"
/transcript_id="NM_001179164.1"
/db_xref="GeneID:856429"
CDS complement(176965..177999)
/gene="PIH1"
/locus_tag="YHR034C"
/gene_synonym="NOP17"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0097255 R2TP
complex [PMID:15766533]"
/experiment="EXISTENCE:genetic interaction:GO:0006364 rRNA
processing [PMID:15670595]"
/experiment="EXISTENCE:genetic interaction:GO:0006457
protein folding [PMID:15766533]"
/experiment="EXISTENCE:mutant phenotype:GO:0000492 box C/D
snoRNP assembly [PMID:18268103]"
/experiment="EXISTENCE:mutant phenotype:GO:0006364 rRNA
processing [PMID:15670595]"
/experiment="EXISTENCE:mutant phenotype:GO:0006457 protein
folding [PMID:15766533]"
/experiment="EXISTENCE:mutant phenotype:GO:0008361
regulation of cell size [PMID:12089449]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:27053109]"
/experiment="EXISTENCE:physical interaction:GO:0006457
protein folding [PMID:15766533]"
/experiment="EXISTENCE:physical interaction:GO:0097255
R2TP complex [PMID:18268103]"
/note="Component of the conserved R2TP complex
(Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90
(Hsp82p and Hsc82p) to mediate assembly large protein
complexes such as box C/D snoRNPs and RNA polymerase II"
/codon_start=1
/product="Pih1p"
/protein_id="NP_011899.1"
/db_xref="GeneID:856429"
/db_xref="SGD:S000001076"
/translation="MADFLLRPIKQRHRNEDKYVSVDAADGSVSKIEPIADFVIKTKL
LSANGPEKLQDGRKVFINVCHSPLVPKPEVDFNARIVFPLIIQNEWEIPIITSCYRMD
HDKKGQECYVWDCCINSDCSRWICDDIQLREILVEWCLESCEIRDSVVLCRDRIAFPK
MKKKGAELPALEVLNDELHQDYKAKMHKIIEEEAGDPMSILRGRNDDGDDNNDPDDGT
LPPLFPIENKISGAKIEEIDKNEIAHRNLKQAPAPAPAPHEQQEDVPEYEVKMKRFKG
AAYKLRILIENKAPNSKPDRFSPSYNFAENILYINGKLSIPLPRDIVVNAADIKIFHI
RKERTLYIYI"
gene <178219..>180111
/gene="NEL1"
/locus_tag="YHR035W"
/db_xref="GeneID:856430"
mRNA <178219..>180111
/gene="NEL1"
/locus_tag="YHR035W"
/product="GTPase-activating protein NEL1"
/transcript_id="NM_001179165.1"
/db_xref="GeneID:856430"
CDS 178219..180111
/gene="NEL1"
/locus_tag="YHR035W"
/experiment="EXISTENCE:direct assay:GO:0005096 GTPase
activator activity [PMID:24947508]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:24947508]"
/note="Activator of Sar1p GTPase activity; paralog of
Sec23 but does not associate with the COPII components;
not an essential gene"
/codon_start=1
/product="GTPase-activating protein NEL1"
/protein_id="NP_011900.1"
/db_xref="GeneID:856430"
/db_xref="SGD:S000001077"
/translation="MCSPTNFLYEPFSSDAVTQNYDQNLKCTKCGAYYSMACSLREQN
VWTCLFCNQSNSNAELPLVPSNTYTLTSAKKEILSRRTIMIIDAICDPHELNYLVSIL
CNNYITRQQEPLSIITIQQSGHVILHNAVNHRRDAVFSINEFMTKYNLDKLNASYFEK
KISEINQESYWFDKSTQGSLRKLLREICKIANKVNISSKRDKRCTGLALFVSSVLASQ
CSLSAYCHIVSFLNGPCTKGGGKVMSRERGESMRQNHHFESKSSQLQLSKSPTKFYKK
MLEKFANQSLIYEFFIASLDQIGILEMSPLITSSMAVSQFDSFNDERFAMSFQKYLNL
RDHNAIYNCHSKIMTAKNAIVVKDFPKYSLNPKNLSLPLEISLGHNSAEAPIQFQTTF
ENQTEKYIRIETLLLPKANRSFGAQNEIVFSMKKIASRIIDSFAYSSKHTKELMKQLF
LLPNQIRGKDVDMVNLIQWCYHIYRSPILSVRNTSPDERYLFLHRIINASKDTCLSLC
KPFIWSYSDLKHDWIVLDVPLTRAQILQDDKTTICVDGGSYLVLRRGKLLEKEGRELC
CKLLNDLQRFPQPLYVETKTGGSQDRFLKSKIIPLDITDKETLGTEDMTFNEYFNLFT
DLSGSK"
gene <180345..>181760
/gene="BRL1"
/locus_tag="YHR036W"
/db_xref="GeneID:856431"
mRNA <180345..>181760
/gene="BRL1"
/locus_tag="YHR036W"
/product="Brl1p"
/transcript_id="NM_001179166.1"
/db_xref="GeneID:856431"
CDS 180345..181760
/gene="BRL1"
/locus_tag="YHR036W"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:15882446|PMID:26432634]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:15882446|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:15882446]"
/experiment="EXISTENCE:genetic interaction:GO:0055088
lipid homeostasis [PMID:26432634]"
/experiment="EXISTENCE:mutant phenotype:GO:0006998 nuclear
envelope organization [PMID:15882446]"
/experiment="EXISTENCE:mutant phenotype:GO:0055088 lipid
homeostasis [PMID:26432634]"
/note="Essential nuclear envelope/ER integral membrane
protein; interacts and functions with Apq12p and Brr6p in
lipid homeostasis; mutants are defective in nuclear pore
complex biogenesis, mRNA nuclear export and are sensitive
to sterol biosynthesis inhibitors and membrane fluidizing
agents; identified as a dosage suppressor of a temperature
sensitive mutation in the major karyopherin, CRM1;
homologous to Brr6p"
/codon_start=1
/product="Brl1p"
/protein_id="NP_011901.1"
/db_xref="GeneID:856431"
/db_xref="SGD:S000001078"
/translation="MESFENLSIRDSFTSGMEHVDEELGGLSDLSISKQGPTLSPQLI
NRFMPHFPSSPSPLRNTLDFSAAKADEEEDDRMEIDEVDDTSFEEEYNNEPIETHTEA
TENAVVEEIEATPEERQKQEKNESQDQSVEEVENIVSPHRSTVIKALLSPTDLGVAAA
TKVEGVVPLPPSANQDDNESSNNNAEGEDIIRNEEVEDEIKSSLGNHKSSQYANAFDS
EIIKRELRSRSKYQPIQVSFNTHNYFYSDKDGIKTYSLTKPNHNKIDEFYDQNEAFKL
PKPWSPNSHPASRASYALMSYLQLFLNAITTVVIFSFILSFIIALQKDLKSTWEQRKH
ELQYESRICQEQYLTNRCNQTPGLPALGEQCAIWKQCMDRNNDIFFRARSTLSAKLFG
DIINSFIDPLNWKTLFVIFCGVITWCFSSNFLLGFVRAKSYYGNGIKTYPLPSSPKSP
TSEETHSSMTASGEDSHLLKQ"
gene <181977..>183704
/gene="PUT2"
/locus_tag="YHR037W"
/db_xref="GeneID:856432"
mRNA <181977..>183704
/gene="PUT2"
/locus_tag="YHR037W"
/product="1-pyrroline-5-carboxylate dehydrogenase"
/transcript_id="NM_001179167.1"
/db_xref="GeneID:856432"
CDS 181977..183704
/gene="PUT2"
/locus_tag="YHR037W"
/EC_number="1.2.1.88"
/experiment="EXISTENCE:direct assay:GO:0003842 L-glutamate
gamma-semialdehyde dehydrogenase activity [PMID:4145181]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:3025596]"
/experiment="EXISTENCE:direct assay:GO:0006537 glutamate
biosynthetic process [PMID:4145181]"
/experiment="EXISTENCE:mutant phenotype:GO:0003842
L-glutamate gamma-semialdehyde dehydrogenase activity
[PMID:387737]"
/experiment="EXISTENCE:mutant phenotype:GO:0010133
L-proline catabolic process to L-glutamate [PMID:387737]"
/note="Delta-1-pyrroline-5-carboxylate dehydrogenase;
nuclear-encoded mitochondrial protein involved in
utilization of proline as sole nitrogen source; deficiency
of human homolog ALDH4A1 causes type II hyperprolinemia
(HPII), an autosomal recessive inborn error of metabolism;
human homolog ALDH4A1 can complement yeast null mutant"
/codon_start=1
/product="1-pyrroline-5-carboxylate dehydrogenase"
/protein_id="NP_011902.1"
/db_xref="GeneID:856432"
/db_xref="SGD:S000001079"
/translation="MLSARCLKSIYFKRSFSQLGHIKPPKHIRNEPVKPFRNIDLKDW
DLLRASLMKFKSSSLEVPLVINGERIYDNNERALFPQTNPANHQQVLANVTQATEKDV
MNAVKAAKDAKKDWYNLPFYDRSAIFLKAADLISTKYRYDMLAATMLGQGKNVYQAEI
DCITELSDFFRYYVKYASDLYAQQPVESADGTWNKAEYRPLEGFVYAVSPFNFTAIAA
NLIGAPALMGNTVVWKPSQTAALSNYLLMTVLEEAGLPKGVINFIPGDPVQVTDQVLA
DKDFGALHFTGSTNVFKSLYGKIQSGVVEGKYRDYPRIIGETGGKNFHLVHPSANISH
AVLSTIRGTFEFQGQKCSAASRLYLPESKSEEFLSDMFGILQSQNVVPMNTSASPISG
GNLRGFMGPVIHEQSFDKLVKVIEDAKKDPELEILYGGQYDKSQGWFVGPTVIKAKRP
DHPYMSTEFFGPILTVYEYPDTEFNEICDIIDNTSQYALTGAIFAKDRKAIEYADEKL
KFSAGNFYINDKCTGAVVSQQWFGGARMSGTDDKAGGPNILSRFVSIRNTKENFYELT
DFKYPSNYE"
gene <184066..>184758
/gene="RRF1"
/locus_tag="YHR038W"
/gene_synonym="FIL1; KIM4"
/db_xref="GeneID:856433"
mRNA <184066..>184758
/gene="RRF1"
/locus_tag="YHR038W"
/gene_synonym="FIL1; KIM4"
/product="Rrf1p"
/transcript_id="NM_001179168.1"
/db_xref="GeneID:856433"
CDS 184066..184758
/gene="RRF1"
/locus_tag="YHR038W"
/gene_synonym="FIL1; KIM4"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:12853640|PMID:24769239|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:mutant phenotype:GO:0032543
mitochondrial translation [PMID:12853640]"
/note="Mitochondrial ribosome recycling factor; essential
for mitochondrial protein synthesis and for the
maintenance of the respiratory function of mitochondria"
/codon_start=1
/product="Rrf1p"
/protein_id="NP_011903.1"
/db_xref="GeneID:856433"
/db_xref="SGD:S000001080"
/translation="MILTTARLNCRPVTVPRLFNRSFSQSFIILKKKSSTPTEKVEED
EIDVNELLKKAETQFKKTLEIQKQKMNEIKQGNFNPKVFNSLVFKNNRKFTDIATTSL
KGKNALLITVFDPKDVKTVISGVLAANLNLTPERVPNNDLQLKVSLPPPTTESRLKVA
KDLKRVFEEYKQSSLKDSLGTIRGSILKEFKSFKKDDAVRKAERDLEKLHKDYVNKLH
DQFQKVEKSIVK"
gene complement(<184875..>186809)
/gene="MSC7"
/locus_tag="YHR039C"
/db_xref="GeneID:856434"
mRNA complement(<184875..>186809)
/gene="MSC7"
/locus_tag="YHR039C"
/product="meiotic recombination directing protein"
/transcript_id="NM_001179169.1"
/db_xref="GeneID:856434"
CDS complement(184875..186809)
/gene="MSC7"
/locus_tag="YHR039C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0007131
reciprocal meiotic recombination [PMID:10511544]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to the endoplasmic
reticulum; msc7 mutants are defective in directing meiotic
recombination events to homologous chromatids"
/codon_start=1
/product="meiotic recombination directing protein"
/protein_id="NP_011904.1"
/db_xref="GeneID:856434"
/db_xref="SGD:S000001081"
/translation="MSKVYLNSDMINHLNSTVQAYFNLWLEKQNAIMRSQPQIIQDNQ
KLIGITTLVASIFTLYVLVKIISTPAKCSSSYKPVKFSLPAPEAAQNNWKGKRSVSTN
IWNPEEPNFIQCHCPATGQYLGSFPSKTEADIDEMVSKAGKAQSTWGNSDFSRRLRVL
ASLHDYILNNQDLIARVACRDSGKTMLDASMGEILVTLEKIQWTIKHGQRALQPSRRP
GPTNFFMKWYKGAEIRYEPLGVISSIVSWNYPFHNLLGPIIAALFTGNAIVVKCSEQV
VWSSEFFVELIRKCLEACDEDPDLVQLCYCLPPTENDDSANYFTSHPGFKHITFIGSQ
PVAHYILKCAAKSLTPVVVELGGKDAFIVLDSAKNLDALSSIIMRGTFQSSGQNCIGI
ERVIVSKENYDDLVKILNDRMTANPLRQGSDIDHLENVDMGAMISDNRFDELEALVKD
AVAKGARLLQGGSRFKHPKYPQGHYFQPTLLVDVTPEMKIAQNEVFGPILVMMKAKNT
DHCVQLANSAPFGLGGSVFGADIKECNYVANSLQTGNVAINDFATFYVCQLPFGGING
SGYGKFGGEEGLLGLCNAKSVCFDTLPFVSTQIPKPLDYPIRNNAKAWNFVKSFIVGA
YTNSTWQRIKSLFSLAKEAS"
gene complement(<187173..>187679)
/gene="VMA10"
/locus_tag="YHR039C-A"
/gene_synonym="YHR039C-B"
/db_xref="GeneID:856435"
mRNA complement(join(<187173..187514,187677..>187679))
/gene="VMA10"
/locus_tag="YHR039C-A"
/gene_synonym="YHR039C-B"
/product="H(+)-transporting V1 sector ATPase subunit G"
/transcript_id="NM_001180027.1"
/db_xref="GeneID:856435"
CDS complement(join(187173..187514,187677..187679))
/gene="VMA10"
/locus_tag="YHR039C-A"
/gene_synonym="YHR039C-B"
/experiment="EXISTENCE:direct assay:GO:0000221 vacuolar
proton-transporting V-type ATPase, V1 domain
[PMID:16774922]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762|PMID:22842922]"
/note="Subunit G of the V1 peripheral membrane domain of
V-ATPase; part of the electrogenic proton pump found
throughout the endomembrane system; involved in vacuolar
acidification; the V1 peripheral membrane domain of the
vacuolar H+-ATPase (V-ATPase) has eight subunits"
/codon_start=1
/product="H(+)-transporting V1 sector ATPase subunit G"
/protein_id="NP_011905.1"
/db_xref="GeneID:856435"
/db_xref="SGD:S000002100"
/translation="MSQKNGIATLLQAEKEAHEIVSKARKYRQDKLKQAKTDAAKEID
SYKIQKDKELKEFEQKNAGGVGELEKKAEAGVQGELAEIKKIAEKKKDDVVKILIETV
IKPSAEVHINAL"
gene <187924..>189024
/gene="BCD1"
/locus_tag="YHR040W"
/db_xref="GeneID:856436"
mRNA <187924..>189024
/gene="BCD1"
/locus_tag="YHR040W"
/product="Bcd1p"
/transcript_id="NM_001179170.1"
/db_xref="GeneID:856436"
CDS 187924..189024
/gene="BCD1"
/locus_tag="YHR040W"
/experiment="EXISTENCE:direct assay:GO:0000492 box C/D
snoRNP assembly [PMID:31537647]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14690591|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0030515 snoRNA
binding [PMID:31537647]"
/experiment="EXISTENCE:direct assay:GO:0042254 ribosome
biogenesis [PMID:31537647]"
/experiment="EXISTENCE:mutant phenotype:GO:0016074
sno(s)RNA metabolic process [PMID:12837249]"
/note="Essential protein required for the accumulation of
box C/D snoRNA"
/codon_start=1
/product="Bcd1p"
/protein_id="NP_011906.1"
/db_xref="GeneID:856436"
/db_xref="SGD:S000001082"
/translation="MAVLCGVCGIKEFKYKCPRCLVQTCSLECSKKHKTRDNCSGQTH
DPKEYISSEALKQADDDKHERNAYVQRDYNYLTQLKRMVHVQKMDARMKNKRVLGPVG
GHNSNFKKRRYDIDEDDRDSTECQRIIRRGVNCLMLPKGMQRSSQNRSKWDKTMDLFV
WSVEWILCPMQEKGEKKELFKHVSHRIKETDFLVQGMGKNVFQKCCEFYRLAGTSSCI
EGEDGSETKEERTQILQKSGLKFYTKTFPYNTTHIMDSKKLVELAIHEKCIGELLKNT
TVIEFPTIFVAMTEADLPEGYEVLHQEPRPLEHTSTLNKFIDNAREEEDAEEDSQPTE
EPVQKETQDASDSDSDSDDDYNPGLSMDFLTA"
gene complement(<189131..>189864)
/gene="SRB2"
/locus_tag="YHR041C"
/gene_synonym="HRS2; MED20"
/db_xref="GeneID:856437"
mRNA complement(join(<189131..189749,189851..>189864))
/gene="SRB2"
/locus_tag="YHR041C"
/gene_synonym="HRS2; MED20"
/product="Srb2p"
/transcript_id="NM_001179171.1"
/db_xref="GeneID:856437"
CDS complement(join(189131..189749,189851..189864))
/gene="SRB2"
/locus_tag="YHR041C"
/gene_synonym="HRS2; MED20"
/experiment="EXISTENCE:direct assay:GO:0001094 TFIID-class
transcription factor complex binding [PMID:1591782]"
/experiment="EXISTENCE:direct assay:GO:0003713
transcription coactivator activity [PMID:1591782]"
/experiment="EXISTENCE:direct assay:GO:0045944 positive
regulation of transcription by RNA polymerase II
[PMID:8187178]"
/experiment="EXISTENCE:direct assay:GO:0051123 RNA
polymerase II preinitiation complex assembly
[PMID:1591782]"
/experiment="EXISTENCE:direct assay:GO:0070847 core
mediator complex [PMID:9891034]"
/experiment="EXISTENCE:genetic interaction:GO:0019904
protein domain specific binding [PMID:2693207]"
/experiment="EXISTENCE:mutant phenotype:GO:0003713
transcription coactivator activity [PMID:15831453]"
/experiment="EXISTENCE:mutant phenotype:GO:0010688
negative regulation of ribosomal protein gene
transcription by RNA polymerase II [PMID:18604275]"
/experiment="EXISTENCE:mutant phenotype:GO:0031669
cellular response to nutrient levels [PMID:18604275]"
/experiment="EXISTENCE:mutant phenotype:GO:0070887
cellular response to chemical stimulus [PMID:18604275]"
/note="Subunit of the RNA polymerase II mediator complex;
associates with core polymerase subunits to form the RNA
polymerase II holoenzyme; general transcription factor
involved in telomere maintenance"
/codon_start=1
/product="Srb2p"
/protein_id="NP_011907.1"
/db_xref="GeneID:856437"
/db_xref="SGD:S000001083"
/translation="MGKSAVIFVERATPATLTELKDALSNSILSVRDPWSIDFRTYRC
SIKNLPADVSKLMYSITFHHHGRQTVLIKDNSAMVTTAAAADIPPALVFNGSSTGVPE
SIDTILSSKLSNIWMQRQLIKGDAGETLILDGLTVRLVNLFSSTGFKGLLIELQADEA
GEFETKIAGIEGHLAEIRAKEYKTSSDSLGPDTSNEICDLAYQYVRALEL"
gene <190543..>192618
/gene="NCP1"
/locus_tag="YHR042W"
/gene_synonym="CPR1"
/db_xref="GeneID:856438"
mRNA <190543..>192618
/gene="NCP1"
/locus_tag="YHR042W"
/gene_synonym="CPR1"
/product="NADPH--hemoprotein reductase"
/transcript_id="NM_001179172.1"
/db_xref="GeneID:856438"
CDS 190543..192618
/gene="NCP1"
/locus_tag="YHR042W"
/gene_synonym="CPR1"
/EC_number="1.6.2.4"
/experiment="EXISTENCE:direct assay:GO:0003958
NADPH-hemoprotein reductase activity [PMID:9087488]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:16407407]"
/experiment="EXISTENCE:direct assay:GO:0009055 electron
transfer activity [PMID:11485306]"
/experiment="EXISTENCE:mutant phenotype:GO:0006696
ergosterol biosynthetic process [PMID:9468503]"
/note="NADP-cytochrome P450 reductase; involved in
ergosterol biosynthesis; associated and coordinately
regulated with Erg11p"
/codon_start=1
/product="NADPH--hemoprotein reductase"
/protein_id="NP_011908.1"
/db_xref="GeneID:856438"
/db_xref="SGD:S000001084"
/translation="MPFGIDNTDFTVLAGLVLAVLLYVKRNSIKELLMSDDGDITAVS
SGNRDIAQVVTENNKNYLVLYASQTGTAEDYAKKFSKELVAKFNLNVMCADVENYDFE
SLNDVPVIVSIFISTYGEGDFPDGAVNFEDFICNAEAGALSNLRYNMFGLGNSTYEFF
NGAAKKAEKHLSAAGAIRLGKLGEADDGAGTTDEDYMAWKDSILEVLKDELHLDEQEA
KFTSQFQYTVLNEITDSMSLGEPSAHYLPSHQLNRNADGIQLGPFDLSQPYIAPIVKS
RELFSSNDRNCIHSEFDLSGSNIKYSTGDHLAVWPSNPLEKVEQFLSIFNLDPETIFD
LKPLDPTVKVPFPTPTTIGAAIKHYLEITGPVSRQLFSSLIQFAPNADVKEKLTLLSK
DKDQFAVEITSKYFNIADALKYLSDGAKWDTVPMQFLVESVPQMTPRYYSISSSSLSE
KQTVHVTSIVENFPNPELPDAPPVVGVTTNLLRNIQLAQNNVNIAETNLPVHYDLNGP
RKLFANYKLPVHVRRSNFRLPSNPSTPVIMIGPGTGVAPFRGFIRERVAFLESQKKGG
NNVSLGKHILFYGSRNTDDFLYQDEWPEYAKKLDGSFEMVVAHSRLPNTKKVYVQDKL
KDYEDQVFEMINNGAFIYVCGDAKGMAKGVSTALVGILSRGKSITTDEATELIKMLKT
SGRYQEDVW"
gene complement(<192805..>193545)
/gene="DOG2"
/locus_tag="YHR043C"
/db_xref="GeneID:856439"
mRNA complement(<192805..>193545)
/gene="DOG2"
/locus_tag="YHR043C"
/product="2-deoxyglucose-6-phosphatase"
/transcript_id="NM_001179173.1"
/db_xref="GeneID:856439"
CDS complement(192805..193545)
/gene="DOG2"
/locus_tag="YHR043C"
/EC_number="3.1.3.68"
/experiment="EXISTENCE:direct assay:GO:0003850
2-deoxyglucose-6-phosphatase activity [PMID:8553694]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006006 glucose
metabolic process [PMID:8553694]"
/note="2-deoxyglucose-6-phosphate phosphatase; member of a
family of low molecular weight phosphatases, induced by
oxidative and osmotic stress, confers 2-deoxyglucose
resistance when overexpressed; DOG2 has a paralog, DOG1,
that arose from a single-locus duplication; the last half
of DOG1 and DOG2 are subject to gene conversions among S.
cerevisiae, S. paradoxus, and S. mikatae"
/codon_start=1
/product="2-deoxyglucose-6-phosphatase"
/protein_id="NP_011909.1"
/db_xref="GeneID:856439"
/db_xref="SGD:S000001085"
/translation="MPQFSVDLCLFDLDGTIVSTTTAAESAWKKLCRQHGVDPVELFK
HSHGARSQEMMKKFFPKLDNTDNKGVLALEKDMADNYLDTVSLIPGAENLLLSLDVDT
ETQKKLPERKWAIVTSGSPYLAFSWFETILKNVGKPKVFITGFDVKNGKPDPEGYSRA
RDLLRQDLQLTGKQDLKYVVFEDAPVGIKAGKAMGAITVGITSSYDKSVLFDAGADYV
VCDLTQVSVVKNNENGIVIQVNNPLTRD"
gene complement(<194068..>194808)
/gene="DOG1"
/locus_tag="YHR044C"
/db_xref="GeneID:856440"
mRNA complement(<194068..>194808)
/gene="DOG1"
/locus_tag="YHR044C"
/product="2-deoxyglucose-6-phosphatase"
/transcript_id="NM_001179174.1"
/db_xref="GeneID:856440"
CDS complement(194068..194808)
/gene="DOG1"
/locus_tag="YHR044C"
/EC_number="3.1.3.68"
/experiment="EXISTENCE:direct assay:GO:0003850
2-deoxyglucose-6-phosphatase activity [PMID:8553694]"
/experiment="EXISTENCE:direct assay:GO:0006006 glucose
metabolic process [PMID:8553694]"
/note="2-deoxyglucose-6-phosphate phosphatase; member of a
family of low molecular weight phosphatases; confers
2-deoxyglucose resistance when overexpressed; DOG1 has a
paralog, DOG2, that arose from a single-locus duplication;
the last half of DOG1 and DOG2 are subject to gene
conversions among S. cerevisiae, S. paradoxus, and S.
mikatae"
/codon_start=1
/product="2-deoxyglucose-6-phosphatase"
/protein_id="NP_011910.1"
/db_xref="GeneID:856440"
/db_xref="SGD:S000001086"
/translation="MAEFSADLCLFDLDGTIVSTTVAAEKAWTKLCYEYGVDPSELFK
HSHGARTQEVLRRFFPKLDDTDNKGVLALEKDIAHSYLDTVSLIPGAENLLLSLDVDT
ETQKKLPERKWAIVTSGSPYLAFSWFETILKNVGKPKVFITGFDVKNGKPDPEGYSRA
RDLLRQDLQLTGKQDLKYVVFEDAPVGIKAGKAMGAITVGITSSYDKSVLFDAGADYV
VCDLTQVSVVKNNENGIVIQVNNPLTRA"
gene <195551..>197233
/gene="DDE1"
/locus_tag="YHR045W"
/db_xref="GeneID:856441"
mRNA <195551..>197233
/gene="DDE1"
/locus_tag="YHR045W"
/product="Dde1p"
/transcript_id="NM_001179175.1"
/db_xref="GeneID:856441"
CDS 195551..197233
/gene="DDE1"
/locus_tag="YHR045W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095|PMID:26928762]"
/note="Protein involved in DNA damage response and
ergosterol biosynthesis; acts in cooperation with Dap1p in
regulating DNA damage response and ergosterol
biosynthesis"
/codon_start=1
/product="Dde1p"
/protein_id="NP_011911.1"
/db_xref="GeneID:856441"
/db_xref="SGD:S000001087"
/translation="MNWSFLLQLVITILLIVLGANWLLSSFLLDFKRDLTGVALSQQS
SISSVRKENETAYYRSILVPTGFPLTTGLGLSLKYKIRNGNFGDVWNAIMEVSKGKNI
IKFTGREKSYSLSELNGMAKRIFPKLSNKNFKNIGIANSIATVEGFTLSLASMMTSIR
TGSIPHFLPAVPRQRLEDVDVLIIDSWKSFKMLNGSEDWYKLIVVCDDPIESLQFDAN
CDVITWKELIDGFTKDTEYQYTPPDDNSDDKKLFAYVTSPWNGTNSFNQICLVSNIAE
FIKGFPLGNELNSNEYLTISTKLANSSASLQIWGKLFAVLLHGGSASFINPTTIDCES
LQETTLLFTETKDVVKLIDSNSRSGLLNKIYLSWATNLLSEGIFTKIARIEPHSLEKL
RCVYLADNVKDAEVISTFPEKIPQLKKTNRRITPSTEQLNKIRAQLGSRVVLELYCPY
AIMGPVAHTNFYDYRVFGKSVDDNVVCYGTLSTTLEGKMVETETNPHLNIEKKQGMLC
IRGFSIGKPVESDRLEKALHLAERFGGGEGWMPLVGVFGLFGQDGCLYIYNQ"
gene complement(<197398..>198285)
/gene="INM1"
/locus_tag="YHR046C"
/db_xref="GeneID:856442"
mRNA complement(<197398..>198285)
/gene="INM1"
/locus_tag="YHR046C"
/product="inositol monophosphate 1-phosphatase INM1"
/transcript_id="NM_001179176.1"
/db_xref="GeneID:856442"
CDS complement(197398..198285)
/gene="INM1"
/locus_tag="YHR046C"
/EC_number="3.1.3.25"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008934 inositol
monophosphate 1-phosphatase activity [PMID:10096091]"
/experiment="EXISTENCE:direct assay:GO:0071545 inositol
phosphate catabolic process [PMID:10844654]"
/experiment="EXISTENCE:genetic interaction:GO:0008934
inositol monophosphate 1-phosphatase activity
[PMID:12593845]"
/experiment="EXISTENCE:genetic interaction:GO:0071545
inositol phosphate catabolic process [PMID:12593845]"
/note="Inositol monophosphatase; involved in biosynthesis
of inositol and in phosphoinositide second messenger
signaling; INM1 expression increases in the presence of
inositol and decreases upon exposure to antibipolar drugs
lithium and valproate"
/codon_start=1
/product="inositol monophosphate 1-phosphatase INM1"
/protein_id="NP_011912.1"
/db_xref="GeneID:856442"
/db_xref="SGD:S000001088"
/translation="MTIDLASIEKFLCELATEKVGPIIKSKSGTQKDYDLKTGSRSVD
IVTAIDKQVEKLIWESVKTQYPTFKFIGEESYVKGETVITDDPTFIIDPIDGTTNFVH
DFPFSCTSLGLTVNKEPVVGVIYNPHINLLVSASKGNGMRVNNKDYDYKSKLESMGSL
ILNKSVVALQPGSAREGKNFQTKMATYEKLLSCDYGFVHGFRNLGSSAMTMAYIAMGY
LDSYWDGGCYSWDVCAGWCILKEVGGRVVGANPGEWSIDVDNRTYLAVRGTINNESDE
QTKYITDFWNCVDGHLKYD"
gene complement(<198740..>201310)
/gene="AAP1"
/locus_tag="YHR047C"
/gene_synonym="AAP1'"
/db_xref="GeneID:856443"
mRNA complement(<198740..>201310)
/gene="AAP1"
/locus_tag="YHR047C"
/gene_synonym="AAP1'"
/product="arginine/alanine aminopeptidase"
/transcript_id="NM_001179177.1"
/db_xref="GeneID:856443"
CDS complement(198740..201310)
/gene="AAP1"
/locus_tag="YHR047C"
/gene_synonym="AAP1'"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0004177
aminopeptidase activity [PMID:8100228]"
/experiment="EXISTENCE:mutant phenotype:GO:0005977
glycogen metabolic process [PMID:8100228]"
/experiment="EXISTENCE:mutant phenotype:GO:0006508
proteolysis [PMID:8100228]"
/note="Arginine/alanine amino peptidase; overproduction
stimulates glycogen accumulation; AAP1 has a paralog,
APE2, that arose from the whole genome duplication"
/codon_start=1
/product="arginine/alanine aminopeptidase"
/protein_id="NP_011913.1"
/db_xref="GeneID:856443"
/db_xref="SGD:S000001089"
/translation="MSREVLPNNVTPLHYDITLEPNFRAFTFEGSLKIDLQINDHSIN
SVQINYLEIDFHSARIEGVNAIEVNKNENQQKATLVFPNGTFENLGPSAKLEIIFSGI
LNDQMAGFYRAKYTDKVTGETKYMATTQMEATDARRAFPCFDEPNLKATFAVTLVSES
FLTHLSNMDVRNETIKEGKKYTTFNTTPKMSTYLVAFIVADLRYVESNNFRIPVRVYS
TPGDEKFGQFAANLAARTLRFFEDTFNIEYPLPKMDMVAVHEFSAGAMENWGLVTYRV
IDLLLDIENSSLDRIQRVAEVIQHELAHQWFGNLVTMDWWEGLWLNEGFATWMSWYSC
NKFQPEWKVWEQYVTDNLQRALNLDSLRSSHPIEVPVNNADEINQIFDAISYSKGSSL
LRMISKWLGEETFIKGVSQYLNKFKYGNAKTGDLWDALADASGKDVCSVMNIWTKRVG
FPVLSVKEHKNKITLTQHRYLSTGDVKEEEDTTIYPILLALKDSTGIDNTLVLNEKSA
TFELKNEEFFKINGDQSGIFITSYSDERWAKLSKQANLLSVEDRVGLVADAKALSASG
YTSTTNFLNLISNWKNEDSFVVWEQIINSLSALKSTWVFEPEDILNALDKFTLDLVLN
KLSELGWNIGEDDSFAIQRLKVTLFSAACTSGNEKMQSIAVEMFEEYANGNKQAIPAL
FKAVVFNTVARLGGENNYEKIFNIYQNPVSSEEKIIALRALGRFEDKELLERTLSYLL
DGTVLNQDFYIPMQGIRVHKKGIERLWAWMQEHWDEIAKRLQPGSPVLGGVLTLGLTN
FTSFEALEKISAFYSRKVTKGFDQTLAQALDTIRSKAQWVSRDREIVATYLREHEYDQ
"
gene <204607..>206151
/gene="YHK8"
/locus_tag="YHR048W"
/db_xref="GeneID:856444"
mRNA <204607..>206151
/gene="YHK8"
/locus_tag="YHR048W"
/product="Yhk8p"
/transcript_id="NM_001179178.1"
/db_xref="GeneID:856444"
CDS 204607..206151
/gene="YHK8"
/locus_tag="YHR048W"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="Presumed antiporter of the major facilitator
superfamily; member of the 12-spanner drug:H(+) antiporter
DHA1 family; expression of gene is up-regulated in cells
exhibiting reduced susceptibility to azoles"
/codon_start=1
/product="Yhk8p"
/protein_id="NP_011914.1"
/db_xref="GeneID:856444"
/db_xref="SGD:S000001090"
/translation="MVAEFQIASAQSSALTSTEEEHCSINSDKAAKLDLELTSERKND
GKQSHEVTFNEDIADPEDIARHMSTARRYYISSLITFTSMVITMISSSWTLPSTHIIE
HFHISHEVSTLGITLYVFGLGIGPLFLSPLSELYGRRITFLYALTLSIIWQCLTIWSK
TITGVMFGRFLSGFFGSAFLSVAGGAIADIFDKDQIGIPMAIYTTSAFLGPSLGPIIG
GALYHQSYKWTFITLLITSGCCLVMIIFTIPETYKPMLLIRKAKRLRKEKNDQRYYAV
LEVTREQTSLLSAIFLSTKRPFGLLLRDRMMGVLCFYTGLELAIIYLYFVAFPYVFKK
LYNFGPMEIACSYIGIMVGMILSAPTCLLFQKTFEWRVKRNNGVKTPEMRFEPLFYGA
FLTPVGLFIFAFTCYKHVHWIAPIIGSAIFGSGVYFVFTGVFAYTVDAYRRYAASGMA
CNTFVRCIMAGVFPLFGLQMYKSMGVNWAGFLLAMVTVAMIPVPFLFTKYGARLRAKS
PYAWDD"
gene <206462..>207193
/gene="FSH1"
/locus_tag="YHR049W"
/db_xref="GeneID:856445"
mRNA <206462..>207193
/gene="FSH1"
/locus_tag="YHR049W"
/product="putative serine hydrolase"
/transcript_id="NM_001179179.1"
/db_xref="GeneID:856445"
CDS 206462..207193
/gene="FSH1"
/locus_tag="YHR049W"
/experiment="EXISTENCE:direct assay:GO:0004622
phosphatidylcholine lysophospholipase A1 activity
[PMID:32920715]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0016788 hydrolase
activity, acting on ester bonds [PMID:32182256]"
/experiment="EXISTENCE:mutant phenotype:GO:0006915
apoptotic process [PMID:31363875]"
/note="Lysophospholipase; hydrolyzes
lysophosphatidylserine to release free fatty acid;
involved in regulated cell death; localizes to both the
nucleus and cytoplasm; contains a catalytic triad of
Ser-His-Asp that is part of an alpha/beta hydrolase fold
and a lipase motif (GXSXG); sequence similarity to Fsh2p
and Fsh3p and the human candidate tumor suppressor and
serine hydrolase, OVCA2"
/codon_start=1
/product="putative serine hydrolase"
/protein_id="NP_011915.1"
/db_xref="GeneID:856445"
/db_xref="SGD:S000001091"
/translation="MTVQIPKLLFLHGFLQNGKVFSEKSSGIRKLLKKANVQCDYIDA
PVLLEKKDLPFEMDDEKWQATLDADVNRAWFYHSEISHELDISEGLKSVVDHIKANGP
YDGIVGFSQGAALSSIITNKISELVPDHPQFKVSVVISGYSFTEPDPEHPGELRITEK
FRDSFAVKPDMKTKMIFIYGASDQAVPSVRSKYLYDIYLKAQNGNKEKVLAYEHPGGH
MVPNKKDIIRPIVEQITSSLQEASE"
gene <207654..>209303
/gene="SMF2"
/locus_tag="YHR050W"
/db_xref="GeneID:856447"
mRNA <207654..>209303
/gene="SMF2"
/locus_tag="YHR050W"
/product="divalent metal ion transporter SMF2"
/transcript_id="NM_001179180.1"
/db_xref="GeneID:856447"
CDS 207654..209303
/gene="SMF2"
/locus_tag="YHR050W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005770 late
endosome [PMID:25713143]"
/experiment="EXISTENCE:direct assay:GO:0005802 trans-Golgi
network [PMID:25713143]"
/experiment="EXISTENCE:direct assay:GO:0022857
transmembrane transporter activity [PMID:10574989]"
/experiment="EXISTENCE:genetic interaction:GO:0006824
cobalt ion transport [PMID:9115231]"
/experiment="EXISTENCE:genetic interaction:GO:0006877
intracellular cobalt ion homeostasis [PMID:9115231]"
/experiment="EXISTENCE:mutant phenotype:GO:0006828
manganese ion transport [PMID:11602606]"
/experiment="EXISTENCE:mutant phenotype:GO:0030026
intracellular manganese ion homeostasis [PMID:11602606]"
/note="Divalent metal ion transporter involved in
manganese homeostasis; has broad specificity for di-valent
and tri-valent metals; post-translationally regulated by
levels of metal ions; member of the Nramp family of metal
transport proteins"
/codon_start=1
/product="divalent metal ion transporter SMF2"
/protein_id="NP_011917.1"
/db_xref="GeneID:856447"
/db_xref="SGD:S000001092"
/translation="MTSQEYEPIQWSDESQTNNDSVNDAYADVNTTHESRRRTTLQPN
STSQSMIGTLRKYARFIGPGLMVSVSYMDPGNYSTAVAAGSAHRYKLLFSVLVSNFMA
AFWQYLCARLGAVTGLDLAQNCKKHLPFGLNITLYILAEMAIIATDLAEVVGTAISLN
ILFHIPLALGVILTVVDVLIVLLAYKPNGSMKGIRIFEAFVSLLVVLTVVCFTVELFY
AKLGPAKEIFSGFLPSKAVFEGDGLYLSLAILGATVMPHSLYLGSGVVQPRLREYDIK
NGHYLPDANDMDNNHDNYRPSYEAISETLHFTITELLISLFTVALFVNCAILIVSGAT
LYGSTQNAEEADLFSIYNLLCSTLSKGAGTVFVLALLFSGQSAGIVCTLSGQMVSEGF
LNWTVSPALRRSATRAVAITPCLILVLVAGRSGLSGALNASQVVLSLLLPFVSAPLLY
FTSSKKIMRVQLNRTKELSRTTDKKPVADRTEDDETIELEEMGIGSSSQERSLVSPAP
EYKDMSNGMIVTVLAIIVWLIISGLNFYMLLGFTTGKEVHL"
gene <209475..>209645
/locus_tag="YHR050W-A"
/db_xref="GeneID:1466528"
mRNA <209475..>209645
/locus_tag="YHR050W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184652.1"
/db_xref="GeneID:1466528"
CDS 209475..209645
/locus_tag="YHR050W-A"
/note="hypothetical protein; identified by expression
profiling and mass spectrometry"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878088.1"
/db_xref="GeneID:1466528"
/db_xref="SGD:S000028832"
/translation="MKRDLIGPVKALIKINNCSCLSRCQISRLPHFFTFHPHFSTLIY
YYNLKNCITSET"
gene <209705..>210151
/gene="COX6"
/locus_tag="YHR051W"
/db_xref="GeneID:856448"
mRNA <209705..>210151
/gene="COX6"
/locus_tag="YHR051W"
/product="cytochrome c oxidase subunit VI"
/transcript_id="NM_001179181.1"
/db_xref="GeneID:856448"
CDS 209705..210151
/gene="COX6"
/locus_tag="YHR051W"
/experiment="EXISTENCE:direct assay:GO:0004129
cytochrome-c oxidase activity [PMID:1331058|PMID:7851399]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0006123
mitochondrial electron transport, cytochrome c to oxygen
[PMID:1331058]"
/experiment="EXISTENCE:direct assay:GO:0045277 respiratory
chain complex IV [PMID:1331058]"
/note="Subunit VI of cytochrome c oxidase (Complex IV);
Complex IV is the terminal member of the mitochondrial
inner membrane electron transport chain; expression is
regulated by oxygen levels"
/codon_start=1
/product="cytochrome c oxidase subunit VI"
/protein_id="NP_011918.1"
/db_xref="GeneID:856448"
/db_xref="SGD:S000001093"
/translation="MLSRAIFRNPVINRTLLRARPGAYHATRLTKNTFIQSRKYSDAH
DEETFEEFTARYEKEFDEAYDLFEVQRVLNNCFSYDLVPAPAVIEKALRAARRVNDLP
TAIRVFEALKYKVENEDQYKAYLDELKDVRQELGVPLKEELFPSSS"
gene <210848..>211978
/gene="CIC1"
/locus_tag="YHR052W"
/gene_synonym="NSA3"
/db_xref="GeneID:856449"
mRNA <210848..>211978
/gene="CIC1"
/locus_tag="YHR052W"
/gene_synonym="NSA3"
/product="Cic1p"
/transcript_id="NM_001179182.1"
/db_xref="GeneID:856449"
CDS 210848..211978
/gene="CIC1"
/locus_tag="YHR052W"
/gene_synonym="NSA3"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:11500370]"
/experiment="EXISTENCE:direct assay:GO:0006364 rRNA
processing [PMID:27643814]"
/experiment="EXISTENCE:direct assay:GO:0019843 rRNA
binding [PMID:27643814]"
/experiment="EXISTENCE:direct assay:GO:0030163 protein
catabolic process [PMID:11500370]"
/experiment="EXISTENCE:direct assay:GO:0030687
preribosome, large subunit precursor
[PMID:11583614|PMID:17443350]"
/experiment="EXISTENCE:direct assay:GO:0042273 ribosomal
large subunit biogenesis [PMID:27643814]"
/experiment="EXISTENCE:direct assay:GO:0043023 ribosomal
large subunit binding [PMID:27643814]"
/experiment="EXISTENCE:direct assay:GO:0070628 proteasome
binding [PMID:11500370]"
/experiment="EXISTENCE:mutant phenotype:GO:0030163 protein
catabolic process [PMID:11500370]"
/experiment="EXISTENCE:mutant phenotype:GO:0030674
protein-macromolecule adaptor activity [PMID:11500370]"
/note="Essential protein that interacts with proteasome
components; has a potential role in proteasome substrate
specificity; also copurifies with 66S pre-ribosomal
particles"
/codon_start=1
/product="Cic1p"
/protein_id="NP_011919.1"
/db_xref="GeneID:856449"
/db_xref="SGD:S000001094"
/translation="MAKKSNSKKSTPVSTPSKEKKKVIEKKSSTAIPRERVIKAVNEL
IKFTSKPQDENNEEGNNGKKNLLEDDEEELKKDLQLIVVNNKSFTGTSKSFKLKLLNV
KHSFYKPWKEASATAVKDFKVLLILKDSDIKKVSEDDLFDQLDSEGIKVDEIICGKDL
KTVYKAYEARNAFISQFSLILADDSIVTSLPKLMGGKAYNKVETTPISIRTHANKEFS
LTTLTNNIKKVYMNQLPVKLPRGTTLNVHLGNLEWLRPEEFVDNVELISEQLIKAYQI
RSIFIKTNRSPVLPLYYNQDVLDELEAKKDKIEETHEDDMVTIDGVQVHLSTFNKGLM
EIANPSELGSIFSKQINNAKKRSSSELEKESSESEAVKKAKS"
gene 212409..213118
/gene="RUF5-1"
/locus_tag="YNCH0007W"
/db_xref="GeneID:9164903"
ncRNA 212409..213118
/ncRNA_class="other"
/gene="RUF5-1"
/locus_tag="YNCH0007W"
/product="RUF5-1"
/note="RNA of Unknown Function"
/transcript_id="NR_132186.1"
/db_xref="GeneID:9164903"
/db_xref="SGD:S000028470"
gene complement(<212519..>212692)
/locus_tag="YHR052C-B"
/db_xref="GeneID:65052908"
mRNA complement(<212519..>212692)
/locus_tag="YHR052C-B"
/product="uncharacterized protein"
/transcript_id="NM_001395031.1"
/db_xref="GeneID:65052908"
CDS complement(212519..212692)
/locus_tag="YHR052C-B"
/note="Novel ORF identified by enrichment-based
proteomics"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001381960.1"
/db_xref="GeneID:65052908"
/db_xref="SGD:S000303807"
/translation="MKVMSANANVVAAKIMNNAKNHVAAQRGVTATTNAPAVTSLKKP
RSHAALGNETNSL"
gene complement(<212535..>212720)
/gene="CUP1-1"
/locus_tag="YHR053C"
/gene_synonym="CUP1"
/db_xref="GeneID:856450"
mRNA complement(<212535..>212720)
/gene="CUP1-1"
/locus_tag="YHR053C"
/gene_synonym="CUP1"
/product="metallothionein CUP1-1"
/transcript_id="NM_001179185.1"
/db_xref="GeneID:856450"
CDS complement(212535..212720)
/gene="CUP1-1"
/locus_tag="YHR053C"
/gene_synonym="CUP1"
/experiment="EXISTENCE:direct assay:GO:0005507 copper ion
binding [PMID:3902832]"
/experiment="EXISTENCE:direct assay:GO:0005758
mitochondrial intermembrane space [PMID:36503220]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:3308865|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0016209 antioxidant
activity [PMID:8367458]"
/experiment="EXISTENCE:direct assay:GO:0019430 removal of
superoxide radicals [PMID:8367458]"
/experiment="EXISTENCE:direct assay:GO:0046870 cadmium ion
binding [PMID:3902832]"
/experiment="EXISTENCE:genetic interaction:GO:0019430
removal of superoxide radicals [PMID:8367458]"
/experiment="EXISTENCE:mutant phenotype:GO:0004784
superoxide dismutase activity [PMID:8367458]"
/experiment="EXISTENCE:mutant phenotype:GO:0010273
detoxification of copper ion [PMID:3536930]"
/experiment="EXISTENCE:mutant phenotype:GO:0046688
response to copper ion [PMID:6370791]"
/experiment="EXISTENCE:mutant phenotype:GO:0071585
detoxification of cadmium ion [PMID:3536930]"
/note="Metallothionein; binds copper and mediates
resistance to high concentrations of copper and cadmium;
locus is variably amplified in different strains, with two
copies, CUP1-1 and CUP1-2, in the genomic sequence
reference strain S288C; CUP1-1 has a paralog, CUP1-2, that
arose from a segmental duplication"
/codon_start=1
/product="metallothionein CUP1-1"
/protein_id="NP_011922.1"
/db_xref="GeneID:856450"
/db_xref="SGD:S000001095"
/translation="MFSELINFQNEGHECQCQCGSCKNNEQCQKSCSCPTGCNSDDKC
PCGNKSEETKKSCCSGK"
rep_origin 212720..213185
/note="ARS810; Putative replication origin; identified in
multiple array studies, not yet confirmed by plasmid-based
assay"
/db_xref="SGD:S000130147"
gene complement(<213185..>214249)
/locus_tag="YHR054C"
/db_xref="GeneID:856451"
mRNA complement(<213185..>214249)
/locus_tag="YHR054C"
/product="uncharacterized protein"
/transcript_id="NM_001179184.1"
/db_xref="GeneID:856451"
CDS complement(213185..214249)
/locus_tag="YHR054C"
/note="hypothetical protein; partial duplicate of
RSC30/YHR056C, truncated remnant of segmental duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011921.1"
/db_xref="GeneID:856451"
/db_xref="SGD:S000001096"
/translation="MVPAAENLSPIPASIDTNDIPLIANDLKLLETQAKLINILQGVP
FYLPVNLTKIESLLETLTMGVSNTVDLYFHDNEVRKEWKDTLNFINTIVYTNFFLFVQ
NESSLSMAVQHSSNNNKTSNSERCAKDLMKIISNMHIFYSITFNFIFPIKSIKSFSSG
NNRFHSNGKEFLFANHFIEILQNFIAITFAIFQRCEVILYDEFYKNLSNEEINVQLLL
IHDKILEILKKIEIIVSFLRDEMNSNGSFKSIKGFNKVLNLIKYMLRFSKKKQNFARN
SDNNNVTDYSQSAKNKNVLLKFPVSELNRIYLKFKEISDFLMEREVVQRSIIIDKDLE
SDNLGITTANFNDFYDAFYN"
gene 214407..215116
/gene="RUF5-2"
/locus_tag="YNCH0008W"
/db_xref="GeneID:9164904"
ncRNA 214407..215116
/ncRNA_class="other"
/gene="RUF5-2"
/locus_tag="YNCH0008W"
/product="RUF5-2"
/note="RNA of Unknown Function"
/transcript_id="NR_132187.1"
/db_xref="GeneID:9164904"
/db_xref="SGD:S000028474"
gene complement(<214517..>214690)
/locus_tag="YHR054C-B"
/db_xref="GeneID:65052909"
mRNA complement(<214517..>214690)
/locus_tag="YHR054C-B"
/product="uncharacterized protein"
/transcript_id="NM_001395032.1"
/db_xref="GeneID:65052909"
CDS complement(214517..214690)
/locus_tag="YHR054C-B"
/note="Novel ORF identified by enrichment-based
proteomics"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001381961.1"
/db_xref="GeneID:65052909"
/db_xref="SGD:S000303808"
/translation="MKVMSANANVVAAKIMNNAKNHVAAQRGVTATTNAPAVTSLKKP
RSHAALGNETNSL"
gene complement(<214533..>214718)
/gene="CUP1-2"
/locus_tag="YHR055C"
/gene_synonym="CUP1"
/db_xref="GeneID:856452"
mRNA complement(<214533..>214718)
/gene="CUP1-2"
/locus_tag="YHR055C"
/gene_synonym="CUP1"
/product="metallothionein CUP1-2"
/transcript_id="NM_001179183.1"
/db_xref="GeneID:856452"
CDS complement(214533..214718)
/gene="CUP1-2"
/locus_tag="YHR055C"
/gene_synonym="CUP1"
/experiment="EXISTENCE:direct assay:GO:0005507 copper ion
binding [PMID:3902832]"
/experiment="EXISTENCE:direct assay:GO:0005758
mitochondrial intermembrane space [PMID:36503220]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:3308865|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0016209 antioxidant
activity [PMID:8367458]"
/experiment="EXISTENCE:direct assay:GO:0019430 removal of
superoxide radicals [PMID:8367458]"
/experiment="EXISTENCE:direct assay:GO:0046870 cadmium ion
binding [PMID:3902832]"
/experiment="EXISTENCE:genetic interaction:GO:0019430
removal of superoxide radicals [PMID:8367458]"
/experiment="EXISTENCE:mutant phenotype:GO:0004784
superoxide dismutase activity [PMID:8367458]"
/experiment="EXISTENCE:mutant phenotype:GO:0010273
detoxification of copper ion [PMID:3536930]"
/experiment="EXISTENCE:mutant phenotype:GO:0046688
response to copper ion [PMID:6370791]"
/experiment="EXISTENCE:mutant phenotype:GO:0071585
detoxification of cadmium ion [PMID:3536930]"
/note="Metallothionein; binds copper and mediates
resistance to high concentrations of copper and cadmium;
locus is variably amplified in different strains, with two
copies, CUP1-1 and CUP1-2, in the genomic sequence
reference strain S288C; CUP1-2 has a paralog, CUP1-1, that
arose from a segmental duplication"
/codon_start=1
/product="metallothionein CUP1-2"
/protein_id="NP_011920.1"
/db_xref="GeneID:856452"
/db_xref="SGD:S000001097"
/translation="MFSELINFQNEGHECQCQCGSCKNNEQCQKSCSCPTGCNSDDKC
PCGNKSEETKKSCCSGK"
rep_origin 214718..215183
/note="ARS811; Putative replication origin; identified in
multiple array studies, not yet confirmed by plasmid-based
assay"
/db_xref="SGD:S000130148"
gene complement(<215183..>217834)
/gene="RSC30"
/locus_tag="YHR056C"
/db_xref="GeneID:856453"
mRNA complement(<215183..>217834)
/gene="RSC30"
/locus_tag="YHR056C"
/product="Rsc30p"
/transcript_id="NM_001179186.2"
/db_xref="GeneID:856453"
CDS complement(215183..217834)
/gene="RSC30"
/locus_tag="YHR056C"
/experiment="EXISTENCE:direct assay:GO:0006303
double-strand break repair via nonhomologous end joining
[PMID:15870268]"
/experiment="EXISTENCE:direct assay:GO:0006337 nucleosome
disassembly [PMID:16492771]"
/experiment="EXISTENCE:direct assay:GO:0006338 chromatin
remodeling [PMID:32188943]"
/experiment="EXISTENCE:direct assay:GO:0006368
transcription elongation by RNA polymerase II
[PMID:17081996]"
/experiment="EXISTENCE:direct assay:GO:0016586 RSC-type
complex [PMID:32188943|PMID:12183366]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:32188943]"
/experiment="EXISTENCE:mutant phenotype:GO:0006303
double-strand break repair via nonhomologous end joining
[PMID:15870268]"
/experiment="EXISTENCE:mutant phenotype:GO:0006355
regulation of DNA-templated transcription [PMID:11336698]"
/note="Component of the RSC chromatin remodeling complex;
non-essential gene required for regulation of ribosomal
protein genes and the cell wall/stress response; null
mutants are osmosensitive; RSC30 has a paralog, RSC3, that
arose from the whole genome duplication"
/codon_start=1
/product="Rsc30p"
/protein_id="NP_011923.2"
/db_xref="GeneID:856453"
/db_xref="SGD:S000001098"
/translation="MMDMQVRKVRKPPACTQCRKRKIGCDRAKPICGNCVKYNKPDCF
YPDGPGKMVAVPSASGMSTHGNGQGSNHFSQGNGVNQKNVMIQTQYPIMQTSIEAFNF
SFNPSVDTAMQWTKAASYQNNNTNNNTAPRQNSSTVSSNVHGNTIVRSDSPDVPSMDQ
IREYNTRLQLVNAQSFDYTDNPYSFNVGINQDSAVFDLMTSPFTQEEVLIKEIDFLKN
KLLDLQSLQLKSLKEKSNLNADNTTANKINKTGENSKKGKVDGKRAGFDHQTSRTSQS
SQKYFTALTITDVQSLVQVKPLKDTPNYLFTKNFIIFRDHYLFKFYNILHDICHINQF
KVSPPNNKNHQQYMEVCKVNFPPKAIIIETLNSESLNNLNIEEFLPIFDKTLLLEFVH
NSFPNGDTCPSFSTVDLPLSQLTKLGELTVLLLLLNDSMTLFNKQAINNHVSALMNNL
RLIRSQITLINLEYYDQETIKFIAITKFYESLYMHDDHKSSLDEDLSCLLSFQIKDFK
LFHFLKKMYYSRHSLLGQSSFMVPAAENLSPIPASIDTNDIPLIANDLKLLETQAKLI
NILQGVPFYLPVNLTKIESLLETLTMGVSNTVDLYFHDNEVRKEWKDTLNFINTIVYT
NFFLFVQNESSLSMAVQHSSNNNKTSNSERCAKDLMKIISNMHIFYSITFNFIFPIKS
IKSFSSGNNRFHSNGKEFLFANHFIEILQNFIAITFAIFQRCEVILYDEFYKNLSNEE
INVQLLLIHDKILEILKKIEIIVSFLRDEMNSNGSFKSIKGFNKVLNLIKYMLRFSKK
KQNFARNSDNNNVTDYSQSAKNKNVLLKFPVSELNRIYLKFKEISDFLMEREVVQRSI
IIDKDLESDNLGITTANFNDFYDAFYN"
gene complement(<218226..>218843)
/gene="CPR2"
/locus_tag="YHR057C"
/gene_synonym="CYP2"
/db_xref="GeneID:856454"
mRNA complement(<218226..>218843)
/gene="CPR2"
/locus_tag="YHR057C"
/gene_synonym="CYP2"
/product="peptidylprolyl isomerase CPR2"
/transcript_id="NM_001179187.1"
/db_xref="GeneID:856454"
CDS complement(218226..218843)
/gene="CPR2"
/locus_tag="YHR057C"
/gene_synonym="CYP2"
/EC_number="5.2.1.8"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0003755
peptidyl-prolyl cis-trans isomerase activity
[PMID:1761234]"
/note="Peptidyl-prolyl cis-trans isomerase (cyclophilin);
catalyzes the cis-trans isomerization of peptide bonds
N-terminal to proline residues; potential role in the
secretory pathway; seamless-GFP and mCherry fusion
proteins localize to the vacuole, while SWAT-GFP fusion
localizes to both the endoplasmic reticulum and vacuole;
suppresses toxicity of slow-folding human Z-type
alpha1-antitrypsin variant associated with liver cirrhosis
and emphysema"
/codon_start=1
/product="peptidylprolyl isomerase CPR2"
/protein_id="NP_011924.1"
/db_xref="GeneID:856454"
/db_xref="SGD:S000001099"
/translation="MKFSGLWCWLLLFLSVNVIASDVGELIDQDDEVITQKVFFDIEH
GEEKVGRIVIGLYGKVCPKTAKNFYKLSTTTNSKKGFIGSTFHRVIPNFMVQGGDFTD
GTGVGGKSIYGDTFPDENFTLKHDRKGRLSMANRGKDTNGSQFFITTTEEASWLDGKH
VVFGQVVDGMDVVNYIQHVSRDANDKPLEAVKIAKCGEWTPELSS"
gene complement(<218997..>219884)
/gene="MED6"
/locus_tag="YHR058C"
/db_xref="GeneID:856455"
mRNA complement(<218997..>219884)
/gene="MED6"
/locus_tag="YHR058C"
/product="mediator complex subunit MED6"
/transcript_id="NM_001179188.1"
/db_xref="GeneID:856455"
CDS complement(218997..219884)
/gene="MED6"
/locus_tag="YHR058C"
/experiment="EXISTENCE:direct assay:GO:0003713
transcription coactivator activity [PMID:9234719]"
/experiment="EXISTENCE:direct assay:GO:0045944 positive
regulation of transcription by RNA polymerase II
[PMID:9234719]"
/experiment="EXISTENCE:direct assay:GO:0051123 RNA
polymerase II preinitiation complex assembly
[PMID:9234719]"
/experiment="EXISTENCE:direct assay:GO:0070847 core
mediator complex [PMID:9891034]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:9234719]"
/note="Subunit of the RNA polymerase II mediator complex;
associates with core polymerase subunits to form the RNA
polymerase II holoenzyme; essential for transcriptional
regulation; protein abundance increases in response to DNA
replication stress"
/codon_start=1
/product="mediator complex subunit MED6"
/protein_id="NP_011925.1"
/db_xref="GeneID:856455"
/db_xref="SGD:S000001100"
/translation="MNVTPLDELQWKSPEWIQVFGLRTENVLDYFAESPFFDKTSNNQ
VIKMQRQFSQLNDPNAAVNMTQNIMTLPDGKNGNLEEEFAYVDPARRQILFKYPMYMQ
LEEELMKLDGTEYVLSSVREPDFWVIRKQRRTNNSGVGSAKGPEIIPLQDYYIIGANI
YQSPTIFKIVQSRLMSTSYHLNSTLESLYDLIEFQPSQGVHYKVPTDTSTTATAATNG
NNAGGGSNKSSVRPTGGANMATVPSTTNVNMTVNTMGTGGQTIDNGTGRTGNGNMGIT
TEMLDKLMVTSIRSTPNYI"
gene <220108..>220500
/gene="FYV4"
/locus_tag="YHR059W"
/gene_synonym="mS41"
/db_xref="GeneID:856456"
mRNA <220108..>220500
/gene="FYV4"
/locus_tag="YHR059W"
/gene_synonym="mS41"
/product="mitochondrial 37S ribosomal protein mS41 FYV4"
/transcript_id="NM_001179189.1"
/db_xref="GeneID:856456"
CDS 220108..220500
/gene="FYV4"
/locus_tag="YHR059W"
/gene_synonym="mS41"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:28154081]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:28154081]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095|PMID:17054397]"
/experiment="EXISTENCE:direct assay:GO:0005763
mitochondrial small ribosomal subunit [PMID:28154081]"
/note="hypothetical protein; required for survival upon
exposure to K1 killer toxin"
/codon_start=1
/product="mitochondrial 37S ribosomal protein mS41 FYV4"
/protein_id="NP_011926.1"
/db_xref="GeneID:856456"
/db_xref="SGD:S000001101"
/translation="MIPSRISHKFPLFLRSSLAAPKAAYRFSSTIPKPSDQVPDVDAF
LNKIGRNCNELKDTFENNWNNLFQWDSKILKEKGVNIQQRKYILKQVHNYRNNRPIHE
IKLGKKSFFGGERKRKAFTAKWKAENKQ"
gene <220725..>221270
/gene="VMA22"
/locus_tag="YHR060W"
/gene_synonym="CEV1; VPH6"
/db_xref="GeneID:856457"
mRNA <220725..>221270
/gene="VMA22"
/locus_tag="YHR060W"
/gene_synonym="CEV1; VPH6"
/product="Vma22p"
/transcript_id="NM_001179190.1"
/db_xref="GeneID:856457"
CDS 220725..221270
/gene="VMA22"
/locus_tag="YHR060W"
/gene_synonym="CEV1; VPH6"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0007035
vacuolar acidification [PMID:1628805]"
/experiment="EXISTENCE:mutant phenotype:GO:0051082
unfolded protein binding [PMID:7673216|PMID:8582630]"
/experiment="EXISTENCE:mutant phenotype:GO:0070072
vacuolar proton-transporting V-type ATPase complex
assembly [PMID:7673216]"
/experiment="EXISTENCE:physical interaction:GO:1990871
Vma12-Vma22 assembly complex [PMID:9660861]"
/note="Protein that is required for vacuolar H+-ATPase
(V-ATPase) function; peripheral membrane protein; not an
actual component of the V-ATPase complex; functions in the
assembly of the V-ATPase; localized to the yeast
endoplasmic reticulum (ER)"
/codon_start=1
/product="Vma22p"
/protein_id="NP_011927.1"
/db_xref="GeneID:856457"
/db_xref="SGD:S000001102"
/translation="MSETRMAQNMDTTDEQYLRLIELLSNYDSTLEQLQKGFQDGYIQ
LSRSNYYNKDSLRGNYGEDYWDETYIGQLMATVEEKNSKVVVEIVKRKAQDKQEKKEE
EDNKLTQRKKGTKPEKQKTQSHKLKQDYDPILMFGGVLSVPSSLRQSQTSFKGCIPLI
AQLINYKNEILTLVETLSEQE"
gene complement(<221534..>222478)
/gene="GIC1"
/locus_tag="YHR061C"
/db_xref="GeneID:856458"
mRNA complement(<221534..>222478)
/gene="GIC1"
/locus_tag="YHR061C"
/product="Gic1p"
/transcript_id="NM_001179191.1"
/db_xref="GeneID:856458"
CDS complement(221534..222478)
/gene="GIC1"
/locus_tag="YHR061C"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:9367979|PMID:9367980]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:9367979|PMID:22842922|PMID:9367980]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:9367979]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:9367980]"
/experiment="EXISTENCE:genetic interaction:GO:0030010
establishment of cell polarity [PMID:9367980]"
/experiment="EXISTENCE:genetic interaction:GO:0031106
septin ring organization [PMID:16371506]"
/experiment="EXISTENCE:genetic interaction:GO:0032488
Cdc42 protein signal transduction [PMID:9367979]"
/experiment="EXISTENCE:mutant phenotype:GO:0007096
regulation of exit from mitosis [PMID:14734533]"
/experiment="EXISTENCE:physical interaction:GO:0031106
septin ring organization [PMID:16371506]"
/experiment="EXISTENCE:physical interaction:GO:0031267
small GTPase binding [PMID:9367979|PMID:9367980]"
/note="Rho-family GTPase interacting protein and Cdc42p
effector; required with Gic2p for polarity establishment
and initiation of budding; involved in recruitment of
septins to the presumptive bud site; interacts with Cdc42p
via the Cdc42/Rac-interactive binding (CRIB) domain; gic1
gic2 double mutants are defective for polarizing Cdc42p at
elevated temperature, so may function both up- and
downstream of Cdc42p; regulates mitotic exit; relocalizes
from bud neck to nucleus upon DNA replication stress"
/codon_start=1
/product="Gic1p"
/protein_id="NP_011928.1"
/db_xref="GeneID:856458"
/db_xref="SGD:S000001103"
/translation="MTEGKRLQQMELPQMKSIWIDEDQEMEKLYGFQVRQRFMNGPST
DSDEDADEDLGIVLVDSKKLALPNKNNIKLPPLPNYMTINPNINSNHKSLTNKKKNFL
GMFKKKDLLSRRHGSAKTAKQSSISTPFDFHHISHANGKREDNPLESHEEKHDVESLV
KFTSLAPQPRPDSNVSSKYSNVVMNDSSRIVSSSTIATTMDSHHDGNETNNTPNGNKQ
LDSPTDLEMTLEDLRNYTFPSVLGDSVSEKTNPSSPSVSSFSGKFKPRELSALHTPEL
GNCFNVDQSLNSPGNRISVDDVLKFYYQCSETSTPRNT"
gene complement(<222877..>223758)
/gene="RPP1"
/locus_tag="YHR062C"
/db_xref="GeneID:856459"
mRNA complement(<222877..>223758)
/gene="RPP1"
/locus_tag="YHR062C"
/product="RNA-binding RNA processing protein RPP1"
/transcript_id="NM_001179192.1"
/db_xref="GeneID:856459"
CDS complement(222877..223758)
/gene="RPP1"
/locus_tag="YHR062C"
/EC_number="3.1.26.5"
/experiment="EXISTENCE:direct assay:GO:0000172
ribonuclease MRP complex [PMID:9620854]"
/experiment="EXISTENCE:direct assay:GO:0000294
nuclear-transcribed mRNA catabolic process, RNase
MRP-dependent [PMID:14729943]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:23700311]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005655 nucleolar
ribonuclease P complex [PMID:9620854]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0034965 intronic
box C/D snoRNA processing [PMID:18713869]"
/experiment="EXISTENCE:mutant phenotype:GO:0000171
ribonuclease MRP activity [PMID:9620854|PMID:9353260]"
/experiment="EXISTENCE:mutant phenotype:GO:0004526
ribonuclease P activity [PMID:9353260|PMID:9620854]"
/experiment="EXISTENCE:mutant phenotype:GO:0006364 rRNA
processing [PMID:9353260|PMID:9620854]"
/experiment="EXISTENCE:mutant phenotype:GO:0008033 tRNA
processing [PMID:9353260|PMID:9620854]"
/experiment="EXISTENCE:physical interaction:GO:0000172
ribonuclease MRP complex [PMID:9353260|PMID:17881380]"
/experiment="EXISTENCE:physical interaction:GO:0005655
nucleolar ribonuclease P complex [PMID:9353260]"
/note="Subunit of both RNase MRP and nuclear RNase P;
RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves
tRNA precursors to generate mature 5' ends and facilitates
turnover of nuclear RNAs; relocalizes to the cytosol in
response to hypoxia"
/codon_start=1
/product="RNA-binding RNA processing protein RPP1"
/protein_id="NP_011929.1"
/db_xref="GeneID:856459"
/db_xref="SGD:S000001104"
/translation="MLVDLNVPWPQNSYADKVTSQAVNNLIKTLSTLHMLGYTHIAIN
FTVNHSEKFPNDVKLLNPIDIKRRFGELMDRTGLKLYSRITLIIDDPSKGQSLSKISQ
AFDIVAALPISEKGLTLSTTNLDIDLLTFQYGSRLPTFLKHKSICSCVNRGVKLEIVY
GYALRDVQARRQFVSNVRSVIRSSRSRGIVIGSGAMSPLECRNILGVTSLIKNLGLPS
DRCSKAMGDLASLVLLNGRLRNKSHKQTIVTGGGSGNGDDVVNDVQGIDDVQTIKVVK
RSMDAEQLGHASKRHKP"
gene complement(<224030..>225169)
/gene="PAN5"
/locus_tag="YHR063C"
/db_xref="GeneID:856460"
mRNA complement(<224030..>225169)
/gene="PAN5"
/locus_tag="YHR063C"
/product="2-dehydropantoate 2-reductase PAN5"
/transcript_id="NM_001179193.1"
/db_xref="GeneID:856460"
CDS complement(224030..225169)
/gene="PAN5"
/locus_tag="YHR063C"
/EC_number="1.1.1.169"
/experiment="EXISTENCE:curator inference:GO:0015940
pantothenate biosynthetic process [PMID:11154694]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="2-dehydropantoate 2-reductase; part of the
pantothenic acid pathway, structurally homologous to E.
coli panE"
/codon_start=1
/product="2-dehydropantoate 2-reductase PAN5"
/protein_id="NP_011930.1"
/db_xref="GeneID:856460"
/db_xref="SGD:S000001105"
/translation="MTAPHRSTIHILGLGAMGTVLAVDLLRFTNALVVPLFRSQERLA
QFQKTNGNNISIRKLYLEGSPIFSYPVEKCECPETFSKKPIDNLVVTTKTYQTKEALA
PYLPYINKNTNLILIQNGLGVLELLREEIFTDSKNRPHLFQGVISHGVYQDKAGVFNH
AGWAGMKIAKLPWTEEEMIQKKSVVEDDAANNSLVKLLTEPKFAKEFGIEHSTYQEML
FGQLFKFLVNACMNPVTAILDCVNGEMKASCGPVFTSIIDECLQILRVAYRPLFQYHE
KYSGNEEYPEMDVNAVLTTDNMVSEVTRIGCDINSRNSSSMRQDTLFLRDIEIEYING
YVVKLADNLNLDPNCCKVNKTIGELATMRLALNRSRSINGDWRKD"
gene complement(<225525..>227141)
/gene="SSZ1"
/locus_tag="YHR064C"
/gene_synonym="PDR13"
/db_xref="GeneID:856461"
mRNA complement(<225525..>227141)
/gene="SSZ1"
/locus_tag="YHR064C"
/gene_synonym="PDR13"
/product="ribosome-associated complex protein SSZ1"
/transcript_id="NM_001179194.1"
/db_xref="GeneID:856461"
CDS complement(225525..227141)
/gene="SSZ1"
/locus_tag="YHR064C"
/gene_synonym="PDR13"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:10792726]"
/experiment="EXISTENCE:mutant phenotype:GO:0002181
cytoplasmic translation [PMID:11929994]"
/experiment="EXISTENCE:mutant phenotype:GO:0006364 rRNA
processing [PMID:20368619]"
/experiment="EXISTENCE:mutant phenotype:GO:0006450
regulation of translational fidelity [PMID:15456889]"
/experiment="EXISTENCE:mutant phenotype:GO:0006452
translational frameshifting [PMID:16607023]"
/experiment="EXISTENCE:mutant phenotype:GO:0051082
unfolded protein binding [PMID:11054575]"
/experiment="EXISTENCE:mutant phenotype:GO:0051083 'de
novo' cotranslational protein folding [PMID:11274393]"
/note="Hsp70 protein that interacts with Zuo1p (a DnaJ
homolog); interacts with Zuo1p to form a
ribosome-associated complex that binds the ribosome via
the Zuo1p subunit; also involved in pleiotropic drug
resistance via sequential activation of PDR1 and PDR5;
binds ATP"
/codon_start=1
/product="ribosome-associated complex protein SSZ1"
/protein_id="NP_011931.2"
/db_xref="GeneID:856461"
/db_xref="SGD:S000001106"
/translation="MSSPVIGITFGNTSSSIAYINPKNDVDVIANPDGERAIPSALSY
VGEDEYHGGQALQQLIRNPKNTIINFRDFIGLPFDKCDVSKCANGAPAVEVDGKVGFV
ISRGEGKEEKLTVDEVVSRHLNRLKLAAEDYIGSAVKEAVLTVPTNFSEEQKTALKAS
AAKIGLQIVQFINEPSAALLAHAEQFPFEKDVNVVVADFGGIRSDAAVIAVRNGIFTI
LATAHDLSLGGDNLDTELVEYFASEFQKKYQANPRKNARSLAKLKANSSITKKTLSNA
TSATISIDSLADGFDYHASINRMRYELVANKVFAQFSSFVDSVIAKAELDPLDIDAVL
LTGGVSFTPKLTTNLEYTLPESVEILGPQNKNASNNPNELAASGAALQARLISDYDAD
ELAEALQPVIVNTPHLKKPIGLIGAKGEFHPVLLAETSFPVQKKLTLKQAKGDFLIGV
YEGDHHIEEKTLEPIPKEENAEEDDESEWSDDEPEVVREKLYTLGTKLMELGIKNANG
VEIIFNINKDGALRVTARDLKTGNAVKGEL"
gene complement(<227532..>229037)
/gene="RRP3"
/locus_tag="YHR065C"
/db_xref="GeneID:856462"
mRNA complement(<227532..>229037)
/gene="RRP3"
/locus_tag="YHR065C"
/product="RNA-dependent ATPase RRP3"
/transcript_id="NM_001179195.1"
/db_xref="GeneID:856462"
CDS complement(227532..229037)
/gene="RRP3"
/locus_tag="YHR065C"
/EC_number="3.6.4.13"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:16449634]"
/experiment="EXISTENCE:direct assay:GO:0003724 RNA
helicase activity [PMID:23153376]"
/experiment="EXISTENCE:direct assay:GO:0008186
ATP-dependent activity, acting on RNA
[PMID:23153376|PMID:18975973]"
/experiment="EXISTENCE:mutant phenotype:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16449634]"
/note="Protein involved in rRNA processing; required for
maturation of the 35S primary transcript of pre-rRNA and
for cleavage leading to mature 18S rRNA; homologous to
eIF-4a, which is a DEAD box RNA-dependent ATPase with
helicase activity"
/codon_start=1
/product="RNA-dependent ATPase RRP3"
/protein_id="NP_011932.2"
/db_xref="GeneID:856462"
/db_xref="SGD:S000001107"
/translation="MSKIVKRKEKKANDELTSLAEKIRAKALENQKKLIEAEKEGGSE
SDSEEDATAEKKKVLKSKSKSTVSTQNENTNEDESFESFSELNLVPELIQACKNLNYS
KPTPIQSKAIPPALEGHDIIGLAQTGSGKTAAFAIPILNRLWHDQEPYYACILAPTRE
LAQQIKETFDSLGSLMGVRSTCIVGGMNMMDQARDLMRKPHIIIATPGRLMDHLENTK
GFSLRKLKFLVMDEADRLLDMEFGPVLDRILKIIPTQERTTYLFSATMTSKIDKLQRA
SLTNPVKCAVSNKYQTVDTLVQTLMVVPGGLKNTYLIYLLNEFIGKTMIIFTRTKANA
ERLSGLCNLLEFSATALHGDLNQNQRMGSLDLFKAGKRSILVATDVAARGLDIPSVDI
VVNYDIPVDSKSYIHRVGRTARAGRSGKSISLVSQYDLELILRIEEVLGKKLPKESVD
KNIILTLRDSVDKANGEVVMEMNRRNKEKIARGKGRRGRMMTRENMDMGER"
gene <229335..>230696
/gene="SSF1"
/locus_tag="YHR066W"
/db_xref="GeneID:856463"
mRNA <229335..>230696
/gene="SSF1"
/locus_tag="YHR066W"
/product="rRNA-binding ribosome biosynthesis protein"
/transcript_id="NM_001179196.1"
/db_xref="GeneID:856463"
CDS 229335..230696
/gene="SSF1"
/locus_tag="YHR066W"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:9611192]"
/experiment="EXISTENCE:direct assay:GO:0019843 rRNA
binding [PMID:11864606]"
/experiment="EXISTENCE:direct assay:GO:0030687
preribosome, large subunit precursor [PMID:11864607]"
/experiment="EXISTENCE:mutant phenotype:GO:0000027
ribosomal large subunit assembly [PMID:11864606]"
/experiment="EXISTENCE:mutant phenotype:GO:0000463
maturation of LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11864607]"
/experiment="EXISTENCE:mutant phenotype:GO:0008361
regulation of cell size [PMID:12089449]"
/note="Constituent of 66S pre-ribosomal particles;
required for ribosomal large subunit maturation;
functionally redundant with Ssf2p; member of the Brix
family; SSF1 has a paralog, SSF2, that arose from the
whole genome duplication"
/codon_start=1
/product="rRNA-binding ribosome biosynthesis protein"
/protein_id="NP_011933.1"
/db_xref="GeneID:856463"
/db_xref="SGD:S000001108"
/translation="MAKRRQKKRTHAQLTPEQEQGIPKSMVIRVGQTSLANHSLNQLV
KDFRQIMQPHTAIKLKERKSNKLKDFVVMCGPLGVTHLFMFTQSEKTGNVSLKIARTP
QGPTVTFQVLDYSLGRDIKKFLKRPKSLNNDDVLNPPLLVLNGFSTSKRSGEDDQDVN
VEKVIVSMFQNIFPPLNPARTSLNSIKRVFMINKDRETGEISMRHYFIDIREVEISRN
LKRLYKAKNNLSKTVPNLHRKEDISSLILDHDLGAYTSESEIEDDAIVRVVDNQDVKA
KHSQSLKSQRTPVEKKDNKEREKETEEEDVEMEEPKPSENLQPTPRKKAIKLTELGPR
LTLKLVKIEEGICSGKVLHHEFVQKSSEEIKALEKRHAAKMRLKEQRKKEQEENIAKK
KAVKDAKKQRKLERRKARAAEGGEGQGKDDAMSDDESSSSDSEHYGSVPEDLDSDLFS
EVE"
gene <230970..>231812
/gene="HTD2"
/locus_tag="YHR067W"
/gene_synonym="RMD12"
/db_xref="GeneID:856464"
mRNA <230970..>231812
/gene="HTD2"
/locus_tag="YHR067W"
/gene_synonym="RMD12"
/product="hydroxyacyl-thioester dehydratase HTD2"
/transcript_id="NM_001179197.1"
/db_xref="GeneID:856464"
CDS 230970..231812
/gene="HTD2"
/locus_tag="YHR067W"
/gene_synonym="RMD12"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:15387819|PMID:16823961|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0019171
(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
activity [PMID:15387819]"
/note="Mitochondrial 3-hydroxyacyl-thioester dehydratase;
involved in fatty acid biosynthesis, required for
respiratory growth and for normal mitochondrial
morphology"
/codon_start=1
/product="hydroxyacyl-thioester dehydratase HTD2"
/protein_id="NP_011934.1"
/db_xref="GeneID:856464"
/db_xref="SGD:S000001109"
/translation="MKSKTWIFRDVLSSHRTKAFDSLLCRRLPVSKATKHLQLGEHFL
FFPPSFEKLDRDGYFNYQNPASLLGNPDLRYRRRIWGQGELVQYLPVTLDQEYTCHES
IKYVKKIRDEHVVCIERTLLQERPENVSSPMDICLFERRVLMYTNSPANKTAVKMPVG
EENYKILKNFTVTDMDIVAYGQMSLNPHRIHWDKEYSRYVEGYDDIIMQGPFSVQLLQ
KCIQPFLEQPIRQLRYRNLNYIYPNTTLSICQSLSSSSGMYTFQIRDLQKANLVYMKA
DVFC"
gene <232133..>233296
/gene="DYS1"
/locus_tag="YHR068W"
/db_xref="GeneID:856465"
mRNA <232133..>233296
/gene="DYS1"
/locus_tag="YHR068W"
/product="deoxyhypusine synthase"
/transcript_id="NM_001179198.1"
/db_xref="GeneID:856465"
CDS 232133..233296
/gene="DYS1"
/locus_tag="YHR068W"
/EC_number="2.5.1.46"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008612
peptidyl-lysine modification to peptidyl-hypusine
[PMID:7629166]"
/experiment="EXISTENCE:direct assay:GO:0034038
deoxyhypusine synthase activity [PMID:7629166]"
/note="Deoxyhypusine synthase; catalyzes formation of
deoxyhypusine, the first step in hypusine biosynthesis;
triggers posttranslational hypusination of translation
elongation factor eIF-5A and regulates its intracellular
levels; tetrameric; human homolog DHPS allows growth of
yeast haploid dys1 null mutant after sporulation of
heterozygous diploid"
/codon_start=1
/product="deoxyhypusine synthase"
/protein_id="NP_011935.1"
/db_xref="GeneID:856465"
/db_xref="SGD:S000001110"
/translation="MSDINEKLPELLQDAVLKASVPIPDDFVKVQGIDYSKPEATNMR
ATDLIEAMKTMGFQASSVGTACEIIDSMRSWRGKHIDELDDHEKKGCFDEEGYQKTTI
FMGYTSNLISSGVRETLRYLVQHKMVDAVVTSAGGVEEDLIKCLAPTYLGEFALKGKS
LRDQGMNRIGNLLVPNDNYCKFEEWIVPILDKMLEEQDEYVKKHGADCLEANQDVDSP
IWTPSKMIDRFGKEINDESSVLYWAHKNKIPIFCPSLTDGSIGDMLFFHTFKASPKQL
RVDIVGDIRKINSMSMAAYRAGMIILGGGLIKHHIANACLMRNGADYAVYINTGQEYD
GSDAGARPDEAVSWGKIKAEAKSVKLFADVTTVLPLIVAATFASGKPIKKVKN"
gene complement(<233579..>234658)
/gene="RRP4"
/locus_tag="YHR069C"
/db_xref="GeneID:856466"
mRNA complement(<233579..>234658)
/gene="RRP4"
/locus_tag="YHR069C"
/product="exosome non-catalytic core subunit RRP4"
/transcript_id="NM_001179199.1"
/db_xref="GeneID:856466"
CDS complement(233579..234658)
/gene="RRP4"
/locus_tag="YHR069C"
/experiment="EXISTENCE:direct assay:GO:0000176 nuclear
exosome (RNase complex) [PMID:19046973|PMID:10465791]"
/experiment="EXISTENCE:direct assay:GO:0000177 cytoplasmic
exosome (RNase complex) [PMID:10465791|PMID:19046973]"
/experiment="EXISTENCE:direct assay:GO:0071038
TRAMP-dependent tRNA surveillance pathway
[PMID:15828860|PMID:17643380]"
/experiment="EXISTENCE:genetic interaction:GO:0071028
nuclear mRNA surveillance [PMID:11586364]"
/experiment="EXISTENCE:mutant phenotype:GO:0000467
exonucleolytic trimming to generate mature 3'-end of 5.8S
rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S
rRNA, LSU-rRNA)
[PMID:9390555|PMID:8600032|PMID:10508172|PMID:10465791]"
/experiment="EXISTENCE:mutant phenotype:GO:0000956
nuclear-transcribed mRNA catabolic process [PMID:9482746]"
/experiment="EXISTENCE:mutant phenotype:GO:0034475 U4
snRNA 3'-end processing [PMID:10611222]"
/experiment="EXISTENCE:mutant phenotype:GO:0071035 nuclear
polyadenylation-dependent rRNA catabolic process
[PMID:10465791]"
/experiment="EXISTENCE:mutant phenotype:GO:0071051
poly(A)-dependent snoRNA 3'-end processing
[PMID:10611222]"
/note="Exosome non-catalytic core component; involved in
3'-5' RNA processing and degradation in both the nucleus
and the cytoplasm; predicted to contain RNA binding
domains; mutations in the human ortholog, EXOSC2, cause
SHRF (Short stature, Hearing loss, Retinitis pigmentosa
and distinctive Facies) syndrome, an RNA exosomopathy"
/codon_start=1
/product="exosome non-catalytic core subunit RRP4"
/protein_id="NP_011936.1"
/db_xref="GeneID:856466"
/db_xref="SGD:S000001111"
/translation="MSEVITITKRNGAFQNSSNLSYNNTGISDDENDEEDIYMHDVNS
ASKSESDSQIVTPGELVTDDPIWMRGHGTYFLDNMTYSSVAGTVSRVNRLLSVIPLKG
RYAPETGDHVVGRIAEVGNKRWKVDIGGKQHAVLMLGSVNLPGGILRRKSESDELQMR
SFLKEGDLLNAEVQSLFQDGSASLHTRSLKYGKLRNGMFCQVPSSLIVRAKNHTHNLP
GNITVVLGVNGYIWLRKTSQMDLARDTPSANNSSSIKSTGPTGAVSLNPSITRLEEES
SWQIYSDENDPSISNNIRQAICRYANVIKALAFCEIGITQQRIVSAYEASMVYSNVGE
LIEKNVMESIGSDILTAEKMRGNGN"
gene <234881..>236380
/gene="TRM5"
/locus_tag="YHR070W"
/db_xref="GeneID:856467"
mRNA <234881..>236380
/gene="TRM5"
/locus_tag="YHR070W"
/product="tRNA (guanine) methyltransferase"
/transcript_id="NM_001179200.1"
/db_xref="GeneID:856467"
CDS 234881..236380
/gene="TRM5"
/locus_tag="YHR070W"
/EC_number="2.1.1.228"
/experiment="EXISTENCE:direct assay:GO:0002939 tRNA
N1-guanine methylation [PMID:17652090]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:17652090|PMID:11914276|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:17652090]"
/experiment="EXISTENCE:direct assay:GO:0052906 tRNA
(guanine(37)-N1)-methyltransferase activity
[PMID:17652090]"
/experiment="EXISTENCE:direct assay:GO:0070901
mitochondrial tRNA methylation [PMID:17652090]"
/experiment="EXISTENCE:mutant phenotype:GO:0002939 tRNA
N1-guanine methylation [PMID:11226173]"
/experiment="EXISTENCE:mutant phenotype:GO:0052906 tRNA
(guanine(37)-N1)-methyltransferase activity
[PMID:11226173]"
/note="tRNA(m(1)G37)methyltransferase; methylates a tRNA
base adjacent to the anticodon that has a role in
prevention of frameshifting; localized to both cytoplasm
and mitochondria, and modifies both cytoplasmic and
mitochondrial tRNAs; mutations in human ortholog TRMT5 are
associated with skeletal muscle respiratory chain
deficiencies, and trm5 mutations analogous to disease
mutations decrease respiration"
/codon_start=1
/product="tRNA (guanine) methyltransferase"
/protein_id="NP_011937.1"
/db_xref="GeneID:856467"
/db_xref="SGD:S000001112"
/translation="MKIALPVFQKFNRLISSCKMSGVFPYNPPVNRQMRELDRSFFIT
KIPMCAVKFPEPKNISVFSKNFKNCILRVPRIPHVVKLNSSKPKDELTSVQNKKLKTA
DGNNTPVTKGVLLHESIHSVEDAYGKLPEDALAFLKENSAEIVPHEYVLDYDFWKAEE
ILRAVLPEQFLEEVPTGFTITGHIAHLNLRTEFKPFDSLIGQVILDKNNKIECVVDKV
SSIATQFRTFPMKVIAGKSDSLVVEQKESNCTFKFDFSKVYWNSRLHTEHERLVKQYF
QPGQVVCDVFAGVGPFAVPAGKKDVIVLANDLNPESYKYLKENIALNKVAKTVKSFNM
DGADFIRQSPQLLQQWIQDEEGGKITIPLPLKKRHRSQQHNDQQPPQPRTKELIIPSH
ISHYVMNLPDSAISFLGNFRGIFAAHTKGATDTIQMPWVHVHCFEKYPPGDQVTEDEL
HARVHARIIAALKVTADDLPLNAVSLHLVRKVAPTKPMYCASFQLPANV"
gene <237004..>237693
/gene="PCL5"
/locus_tag="YHR071W"
/db_xref="GeneID:856468"
mRNA <237004..>237693
/gene="PCL5"
/locus_tag="YHR071W"
/product="Pcl5p"
/transcript_id="NM_001179201.1"
/db_xref="GeneID:856468"
CDS 237004..237693
/gene="PCL5"
/locus_tag="YHR071W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:19218424]"
/experiment="EXISTENCE:direct assay:GO:0016538
cyclin-dependent protein serine/threonine kinase regulator
activity [PMID:12101234]"
/experiment="EXISTENCE:mutant phenotype:GO:0016242
negative regulation of macroautophagy [PMID:20417603]"
/experiment="EXISTENCE:mutant phenotype:GO:0031647
regulation of protein stability [PMID:12101234]"
/experiment="EXISTENCE:physical interaction:GO:0000307
cyclin-dependent protein kinase holoenzyme complex
[PMID:16611745]"
/note="Cyclin; interacts with and phosphorylated by Pho85p
cyclin-dependent kinase (Cdk), induced by Gcn4p at level
of transcription, specifically required for Gcn4p
degradation, may be sensor of cellular protein
biosynthetic capacity"
/codon_start=1
/product="Pcl5p"
/protein_id="NP_011938.1"
/db_xref="GeneID:856468"
/db_xref="SGD:S000001113"
/translation="MDGNHRFTPDSKEFNTVVKSKESSTGRNPYQTPPLEHNGTHHQT
NYSRKKTNLAIIISNFLSEISRPLSNGKINNSTHNILKFLNEVLKRSKCSKENAVLAT
FYFQKIHQSRGVRDESSLPEFSHCSRRIFLCCLILSHKFLNDNTYSMKNWQIISGLHA
KDLSLMERWCLGKLNYELAIPYDEFLLWETNTLMKAKLRVGTPANAPVKRPRESDNDY
DANSWKQIKSC"
gene complement(237848..237939)
/locus_tag="YNCH0009C"
/db_xref="GeneID:856469"
tRNA complement(join(237848..237883,237903..237939))
/locus_tag="YNCH0009C"
/product="tRNA-Phe"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:10024171]"
/note="Phenylalanine tRNA (tRNA-Phe), predicted by
tRNAscan-SE analysis"
/db_xref="GeneID:856469"
/db_xref="SGD:S000006566"
gene <239098..>241293
/gene="ERG7"
/locus_tag="YHR072W"
/db_xref="GeneID:856470"
mRNA <239098..>241293
/gene="ERG7"
/locus_tag="YHR072W"
/product="lanosterol synthase ERG7"
/transcript_id="NM_001179202.2"
/db_xref="GeneID:856470"
CDS 239098..241293
/gene="ERG7"
/locus_tag="YHR072W"
/EC_number="5.4.99.7"
/experiment="EXISTENCE:direct assay:GO:0000250 lanosterol
synthase activity [PMID:12842197]"
/experiment="EXISTENCE:direct assay:GO:0005811 lipid
droplet [PMID:10515935|PMID:24868093|PMID:11706015]"
/experiment="EXISTENCE:direct assay:GO:0006696 ergosterol
biosynthetic process [PMID:12842197]"
/experiment="EXISTENCE:mutant phenotype:GO:0000250
lanosterol synthase activity [PMID:18842050]"
/experiment="EXISTENCE:mutant phenotype:GO:0006696
ergosterol biosynthetic process [PMID:200835]"
/note="Lanosterol synthase; an essential enzyme that
catalyzes the cyclization of squalene 2,3-epoxide, a step
in ergosterol biosynthesis; human LSS functionally
complements the lethality of the erg7 null mutation"
/codon_start=1
/product="lanosterol synthase ERG7"
/protein_id="NP_011939.2"
/db_xref="GeneID:856470"
/db_xref="SGD:S000001114"
/translation="MTEFYSDTIGLPKTDPRLWRLRTDELGRESWEYLTPQQAANDPP
STFTQWLLQDPKFPQPHPERNKHSPDFSAFDACHNGASFFKLLQEPDSGIFPCQYKGP
MFMTIGYVAVNYIAGIEIPEHERIELIRYIVNTAHPVDGGWGLHSVDKSTVFGTVLNY
VILRLLGLPKDHPVCAKARSTLLRLGGAIGSPHWGKIWLSALNLYKWEGVNPAPPETW
LLPYSLPMHPGRWWVHTRGVYIPVSYLSLVKFSCPMTPLLEELRNEIYTKPFDKINFS
KNRNTVCGVDLYYPHSTTLNIANSLVVFYEKYLRNRFIYSLSKKKVYDLIKTELQNTD
SLCIAPVNQAFCALVTLIEEGVDSEAFQRLQYRFKDALFHGPQGMTIMGTNGVQTWDC
AFAIQYFFVAGLAERPEFYNTIVSAYKFLCHAQFDTECVPGSYRDKRKGAWGFSTKTQ
GYTVADCTAEAIKAIIMVKNSPVFSEVHHMISSERLFEGIDVLLNLQNIGSFEYGSFA
TYEKIKAPLAMETLNPAEVFGNIMVEYPYVECTDSSVLGLTYFHKYFDYRKEEIRTRI
RIAIEFIKKSQLPDGSWYGSWGICFTYAGMFALEALHTVGETYENSSTVRKGCDFLVS
KQMKDGGWGESMKSSELHSYVDSEKSLVVQTAWALIALLFAEYPNKEVIDRGIDLLKN
RQEESGEWKFESVEGVFNHSCAIEYPSYRFLFPIKALGMYSRAYETHTL"
gene <241664..>241840
/gene="NOP10"
/locus_tag="YHR072W-A"
/db_xref="GeneID:856471"
mRNA <241664..>241840
/gene="NOP10"
/locus_tag="YHR072W-A"
/product="snoRNP complex protein NOP10"
/transcript_id="NM_001184438.1"
/db_xref="GeneID:856471"
CDS 241664..241840
/gene="NOP10"
/locus_tag="YHR072W-A"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:9843512]"
/experiment="EXISTENCE:direct assay:GO:0031120 snRNA
pseudouridine synthesis [PMID:15962000]"
/experiment="EXISTENCE:mutant phenotype:GO:0006364 rRNA
processing [PMID:9843512]"
/experiment="EXISTENCE:mutant phenotype:GO:0031118 rRNA
pseudouridine synthesis [PMID:9843512]"
/experiment="EXISTENCE:physical interaction:GO:0031429 box
H/ACA snoRNP complex [PMID:9843512]"
/note="Subunit of box H/ACA snoRNP complex; required for
pseudouridylation and processing of pre-18S rRNA"
/codon_start=1
/product="snoRNP complex protein NOP10"
/protein_id="NP_058135.1"
/db_xref="GeneID:856471"
/db_xref="SGD:S000007455"
/translation="MHLMYTLGPDGKRIYTLKKVTESGEITKSAHPARFSPDDKYSRQ
RVTLKKRFGLVPGQ"
gene <242582..>245572
/gene="OSH3"
/locus_tag="YHR073W"
/db_xref="GeneID:856472"
mRNA <242582..>245572
/gene="OSH3"
/locus_tag="YHR073W"
/product="oxysterol-binding protein related protein OSH3"
/transcript_id="NM_001179203.1"
/db_xref="GeneID:856472"
CDS 242582..245572
/gene="OSH3"
/locus_tag="YHR073W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11408574]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:25420878]"
/experiment="EXISTENCE:direct assay:GO:0008289 lipid
binding [PMID:20008566]"
/experiment="EXISTENCE:direct assay:GO:0015918 sterol
transport [PMID:20008566]"
/experiment="EXISTENCE:direct assay:GO:0032541 cortical
endoplasmic reticulum [PMID:20008566]"
/experiment="EXISTENCE:direct assay:GO:0120015 sterol
transfer activity [PMID:20008566]"
/experiment="EXISTENCE:genetic interaction:GO:0000742
karyogamy involved in conjugation with cellular fusion
[PMID:12054531]"
/experiment="EXISTENCE:genetic interaction:GO:0001403
invasive growth in response to glucose limitation
[PMID:12054531]"
/experiment="EXISTENCE:genetic interaction:GO:0006887
exocytosis [PMID:17004323]"
/experiment="EXISTENCE:genetic interaction:GO:0006897
endocytosis [PMID:15173322]"
/experiment="EXISTENCE:genetic interaction:GO:0030011
maintenance of cell polarity [PMID:17004323]"
/experiment="EXISTENCE:genetic interaction:GO:0034727
piecemeal microautophagy of the nucleus [PMID:15367582]"
/experiment="EXISTENCE:genetic interaction:GO:0061709
reticulophagy [PMID:35101986]"
/experiment="EXISTENCE:mutant phenotype:GO:0007124
pseudohyphal growth [PMID:17082746|PMID:12054531]"
/experiment="EXISTENCE:mutant phenotype:GO:0015918 sterol
transport [PMID:16585271]"
/experiment="EXISTENCE:mutant phenotype:GO:0035621 ER to
Golgi ceramide transport [PMID:24213531]"
/note="Member of an oxysterol-binding protein family; this
family has seven members in S. cerevisiae; family members
have overlapping, redundant functions in sterol metabolism
and collectively perform a function essential for
viability; contains FFAT motif; interacts with ER anchor
Scs2p at patches at the plasma membrane; regulated by
sterol binding"
/codon_start=1
/product="oxysterol-binding protein related protein OSH3"
/protein_id="NP_011940.1"
/db_xref="GeneID:856472"
/db_xref="SGD:S000001115"
/translation="METIDIQNRSFVVRWVKCGRGDVINYQIKPLKKSIEVGIYKKLK
SSVDDHASAVHIAPDTKTLLDYTTKSLLHKGSSSNIEEHHRRSSQHSHSSSNGSDNKR
KERSYSSLSISGIQQQSQEIPLREKLSASGFTLVKRVGNVSGNTMVQGDLEVKDTDYY
YAFILDNSSSKNAKKKILFNASVINGDNQSMISTRSTPPARPTALSRTSTQQDMLFRV
GQGRYLQGYLLKKRRKRLQGFKKRFFTLDFRYGTLSYYLNDHNQTCRGEIVISLSSVS
ANKKDKIIIIDSGMEVWVLKATTKENWQSWVDALQTCFDDQFEDKDTSTLEENPDILD
DDKEVINKSSPQDHDHLTPTATTKSALSHRQHTQKDMDDIYVPLPSESYATFSMNLRL
IQQRLEQCKKDSLSYKPTTLHQRSEGLNGTHSSSSVFTNNRVSSFNHSSSGMTSSDSL
ASEEVPSNKTYIEHALYNQLADLEVFVSRFVTQGEVLFKDHQILCKKAKDTRVSLTSY
LSENDEFFDAEEEISRGVIILPDTEDDINNIVEETPLLGKSDQNEFTKEVQLSGSEQI
ASSSVESYTTNDENHSRKHLKNRHKNRRRGHPHHQKTKSAQSSTETFTSKDLFALSYP
KSVTRRNDIPEAAASPPSLLSFLRKNVGKDLSSIAMPVTSNEPISILQLISETFEYAP
LLTKATQRPDPITFVSAFAISFLSIYRDKTRTLRKPFNPLLAETFELIREDMGFRLIS
EKVSHRPPVFAFFAEHLDWECSYTVTPSQKFWGKSIELNNEGILRLKFKTTGELFEWT
QPTTILKNLIAGERYMEPVNEFEVHSSKGDKSHILFDKAGMFSGRSEGFKVSIIPPPS
SNRKKETLAGKWTQSLANETTHETIWEVGDLVSNPKKKYGFTKFTANLNEITEIEKGN
LPPTDSRLRPDIRAYEEGNVDKAEEWKLKLEQLQRERRNKGQDVEPKYFEKVSKNEWK
YITGPKSYWERRKKHDWSDISQLW"
rep_origin 245773..245847
/note="ARS813; Autonomously Replicating Sequence"
/db_xref="SGD:S000118359"
gene <246193..>248337
/gene="QNS1"
/locus_tag="YHR074W"
/db_xref="GeneID:856473"
mRNA <246193..>248337
/gene="QNS1"
/locus_tag="YHR074W"
/product="glutamine-dependent NAD(+) synthetase"
/transcript_id="NM_001179204.1"
/db_xref="GeneID:856473"
CDS 246193..248337
/gene="QNS1"
/locus_tag="YHR074W"
/EC_number="6.3.5.1"
/experiment="EXISTENCE:direct assay:GO:0003952 NAD+
synthase (glutamine-hydrolyzing) activity
[PMID:12898714|PMID:12771147]"
/experiment="EXISTENCE:direct assay:GO:0004359 glutaminase
activity [PMID:12771147]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12898714]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:12898714]"
/experiment="EXISTENCE:direct assay:GO:0009435 NAD+
biosynthetic process [PMID:12898714|PMID:12771147]"
/experiment="EXISTENCE:mutant phenotype:GO:0003952 NAD+
synthase (glutamine-hydrolyzing) activity
[PMID:12898714|PMID:12771147]"
/experiment="EXISTENCE:mutant phenotype:GO:0004359
glutaminase activity [PMID:12771147]"
/experiment="EXISTENCE:mutant phenotype:GO:0009435 NAD+
biosynthetic process [PMID:12771147|PMID:12898714]"
/note="Glutamine-dependent NAD(+) synthetase; essential
for the formation of NAD(+) from nicotinic acid adenine
dinucleotide"
/codon_start=1
/product="glutamine-dependent NAD(+) synthetase"
/protein_id="NP_011941.1"
/db_xref="GeneID:856473"
/db_xref="SGD:S000001116"
/translation="MSHLITLATCNLNQWALDFEGNRDRILQSIKIAKERGARLRVGP
ELEITGYGCLDHFLENDVCLHSWEMYAQIIKNKETHGLILDIGMPVLHKNVRYNCRLL
SLDGEILFIRPKIWLANDGNYREMRFFTPWMKPGVVEDFILPPEIQKVTGQRLVPFGD
AVINSLDTCIGTETCEELFTPQSPHIAMSLDGVEIMTNSSGSHHELRKLNKRLDLILN
ATKRCGGVYLYANQRGCDGDRLYYDGCALIAINGTIVAQGSQFSLDDVEVVTATVDLE
EVRSYRAAVMSRGLQASLAEIKFKRIDIPVELALMTSRFDPTVCPTKVREPFYHSPEE
EIALGPACWMWDYLRRCNGTGFFLPLSGGIDSCATAMIVHSMCRLVTDAAQNGNEQVI
KDVRKITRSGDDWIPDSPQDLASKIFHSCFMGTENSSKETRNRAKDLSNAIGSYHVDL
KMDSLVSSVVSLFEVATGKKPIYKIFGGSQIENLALQNIQARLRMVLSYLFAQLLPWV
RGIPNSGGLLVLGSANVDECLRGYLTKYDCSSADINPIGGISKTDLKRFIAYASKQYN
MPILNDFLNATPTAELEPMTKDYVQSDEIDMGMTYEELGVFGYLRKVEKCGPYSMFLK
LLHQWSPKLTPRQISEKVKRFFFFYAINRHKQTVLTPSYHAEQYSPEDNRFDLRPFLI
NPRFPWASRKIDEVVEQCEAHKGSTLDIMSID"
gene complement(<248439..>249641)
/gene="PPE1"
/locus_tag="YHR075C"
/db_xref="GeneID:856474"
mRNA complement(<248439..>249641)
/gene="PPE1"
/locus_tag="YHR075C"
/product="phosphoprotein phosphatase methylesterase 1"
/transcript_id="NM_001179205.1"
/db_xref="GeneID:856474"
CDS complement(248439..249641)
/gene="PPE1"
/locus_tag="YHR075C"
/EC_number="3.1.1.89"
/experiment="EXISTENCE:direct assay:GO:0005763
mitochondrial small ribosomal subunit [PMID:9151978]"
/experiment="EXISTENCE:mutant phenotype:GO:0051723 protein
methylesterase activity [PMID:11060018]"
/experiment="EXISTENCE:mutant phenotype:GO:1990577
C-terminal protein demethylation [PMID:11060018]"
/note="Protein with carboxyl methyl esterase activity; may
have a role in demethylation of the phosphoprotein
phosphatase catalytic subunit; also identified as a small
subunit mitochondrial ribosomal protein"
/codon_start=1
/product="phosphoprotein phosphatase methylesterase 1"
/protein_id="NP_011942.1"
/db_xref="GeneID:856474"
/db_xref="SGD:S000001117"
/translation="MSDDLRRKIALSQFERAKNVLDATFQEAYEDDENDGDALGSLPS
FNGQSNRNRKYTGKTGSTTDRISSKEKSSLPTWSDFFDNKELVSLPDRDLDVNTYYTL
PTSLLSNTTSIPIFIFHHGAGSSGLSFANLAKELNTKLEGRCGCFAFDARGHAETKFK
KADAPICFDRDSFIKDFVSLLNYWFKSKISQEPLQKVSVILIGHSLGGSICTFAYPKL
STELQKKILGITMLDIVEEAAIMALNKVEHFLQNTPNVFESINDAVDWHVQHALSRLR
SSAEIAIPALFAPLKSGKVVRITNLKTFSPFWDTWFTDLSHSFVGLPVSKLLILAGNE
NLDKELIVGQMQGKYQLVVFQDSGHFIQEDSPIKTAITLIDFWKRNDSRNVVIKTNWG
QHKTVQNT"
gene <251101..>252225
/gene="PTC7"
/locus_tag="YHR076W"
/db_xref="GeneID:856475"
mRNA join(<251101..251155,251249..>252225)
/gene="PTC7"
/locus_tag="YHR076W"
/product="type 2C protein phosphatase PTC7"
/transcript_id="NM_001179206.1"
/db_xref="GeneID:856475"
CDS join(251101..251155,251249..252225)
/gene="PTC7"
/locus_tag="YHR076W"
/EC_number="3.1.3.16"
/experiment="EXISTENCE:direct assay:GO:0004721
phosphoprotein phosphatase activity [PMID:23940037]"
/experiment="EXISTENCE:direct assay:GO:0004722 protein
serine/threonine phosphatase activity
[PMID:23940037|PMID:12220683]"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:19564484]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:19564484|PMID:18180296|PMID:11327310|PMID:16823961|P
MID:14576278|PMID:23940037|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0006470 protein
dephosphorylation [PMID:23940037]"
/note="Type 2C serine/threonine protein phosphatase
(PP2C); alternatively spliced to create two mRNA isoforms;
protein from spliced form localizes to the mitochondria
while the one from the unspliced form is localized to the
nuclear envelope; activates coenzyme Q6 biosynthesis by
dephosphorylation of demethoxy-Q6 hydroxylase Coq7p"
/codon_start=1
/product="type 2C protein phosphatase PTC7"
/protein_id="NP_011943.2"
/db_xref="GeneID:856475"
/db_xref="SGD:S000001118"
/translation="MFANVGFRTLRVSRGPLYGSCSQIISFSKRTFYSSAKSGYQSNN
SHGDAYSSGSQSGPFTYKTAVAFQPKDRDDLIYQKLKDSIRSPTGEDNYFVTSNNVHD
IFAGVADGVGGWAEHGYDSSAISRELCKKMDEISTALAENSSKETLLTPKKIIGAAYA
KIRDEKVVKVGGTTAIVAHFPSNGKLEVANLGDSWCGVFRDSKLVFQTKFQTVGFNAP
YQLSIIPEEMLKEAERRGSKYILNTPRDADEYSFQLKKKDIIILATDGVTDNIATDDI
ELFLKDNAARTNDELQLLSQKFVDNVVSLSKDPNYPSVFAQEISKLTGKNYSGGKEDD
ITVVVVRVD"
gene complement(<252374..>255756)
/gene="NMD2"
/locus_tag="YHR077C"
/gene_synonym="IFS1; SUA1; SUP111; UPF2"
/db_xref="GeneID:856476"
mRNA complement(join(<252374..255637,255751..>255756))
/gene="NMD2"
/locus_tag="YHR077C"
/gene_synonym="IFS1; SUA1; SUP111; UPF2"
/product="Nmd2p"
/transcript_id="NM_001179207.1"
/db_xref="GeneID:856476"
CDS complement(join(252374..255637,255751..255756))
/gene="NMD2"
/locus_tag="YHR077C"
/gene_synonym="IFS1; SUA1; SUP111; UPF2"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:16043493]"
/experiment="EXISTENCE:genetic interaction:GO:0070478
nuclear-transcribed mRNA catabolic process, 3'-5'
exonucleolytic nonsense-mediated decay [PMID:12881429]"
/experiment="EXISTENCE:mutant phenotype:GO:0000184
nuclear-transcribed mRNA catabolic process,
nonsense-mediated decay [PMID:16611983]"
/experiment="EXISTENCE:mutant phenotype:GO:0006310 DNA
recombination [PMID:23390378]"
/note="Protein involved in the nonsense-mediated mRNA
decay (NMD) pathway; acts as a scaffold protein bridging
Nam7p and Upf3p; involved in telomere maintenance;
contains an acidic domain and three middle domains of
eukaryotic initiation factor 4G (mIF4G) with aspartic 59
of mIF4G-1 important for NMD activity and translation
termination accuracy"
/codon_start=1
/product="Nmd2p"
/protein_id="NP_011944.2"
/db_xref="GeneID:856476"
/db_xref="SGD:S000001119"
/translation="MDDGRKKELHDLNTRAWNGEEVFPLKSKKLDSSIKRNTGFIKKL
KKGFVKGSESSLLKDLSEASLEKYLSEIIVTVTECLLNVLNKNDDVIAAVEIISGLHQ
RFNGRFTSPLLGAFLQAFENPSVDIESERDELQRITRVKGNLRVFTELYLVGVFRTLD
DIESKDAIPNFLQKKTGRKDPLLFSILREILNYKFKLGFTTTIATAFIKKFAPLFRDD
DNSWDDLIYDSKLKGALQSLFKNFIDATFARATELHKKVNKLQREHQKCQIRTGKLRD
EYVEEYDKLLPIFIRFKTSAITLGEFFKLEIPELEGASNDDLKETASPMITNQILPPN
QRLWENEDTRKFYEILPDISKTVEESQSSKTEKDSNVNSKNINLFFTDLEMADCKDII
DDLSNRYWSSYLDNKATRNRILKFFMETQDWSKLPVYSRFIATNSKYMPEIVSEFINY
LDNGFRSQLHSNKINVKNIIFFSEMIKFQLIPSFMIFHKIRTLIMYMQVPNNVEILTV
LLEHSGKFLLNKPEYKELMEKMVQLIKDKKNDRQLNMNMKSALENIITLLYPPSVKSL
NVTVKTITPEQQFYRILIRSELSSLDFKHIVKLVRKAHWDDVAIQKVLFSLFSKPHKI
SYQNIPLLTKVLGGLYSYRRDFVIRCIDQVLENIERGLEINDYGQNMHRISNVRYLTE
IFNFEMIKSDVLLDTIYHIIRFGHINNQPNPFYLNYSDPPDNYFRIQLVTTILLNINR
TPAAFTKKCKLLLRFFEYYTFIKEQPLPKETEFRVSSTFKKYENIFGNTKFERSENLV
ESASRLESLLKSLNAIKSKDDRVKGSSASIHNGKESAVPIESITEDDEDEDDENDDGV
DLLGEDEDAEISTPNTESAPGKHQAKQDESEDEDDEDDDEDDDDDDDDDDDDGEEGDE
DDDEDDDDEDDDDEEEEDSDSDLEYGGDLDADRDIEMKRMYEEYERKLKDEEERKAEE
ELERQFQKMMQESIDARKSEKVVASKIPVISKPVSVQKPLLLKKSEEPSSSKETYEEL
SKPKKIAFTFLTKSGKKTQSRILQLPTDVKFVSDVLEEEEKLKTERNKIKKIVLKRSF
D"
gene <256360..>258018
/locus_tag="YHR078W"
/db_xref="GeneID:856477"
mRNA <256360..>258018
/locus_tag="YHR078W"
/product="uncharacterized protein"
/transcript_id="NM_001179208.1"
/db_xref="GeneID:856477"
CDS 256360..258018
/locus_tag="YHR078W"
/note="High osmolarity-regulated gene of unknown function"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011945.1"
/db_xref="GeneID:856477"
/db_xref="SGD:S000001120"
/translation="MEALIVFIVLSVSGAFAYKCSYERLWFKVGSLFDIISTSSKKNV
IPLASKMEVGSNEDVSSMGNFINKFYTEYSLPSHKVLQSLRVLFSLAMMTYTVTIEII
LWQIKVAGMDKEVTFITTWVWPLTAIMLSFILILFQPFFIIISLLNKFYNDKFDIDRL
IIVTCIILSTLIALLSYINIGPFQYTKNILTRLSIGGVTVMASLSGLATVSSLYYNFL
VIWHKFRNTPMSDPSFRNINNSNNNSKSLLWTTDAYIEEKIQDYEHNIEQNVQILRSL
EEEVGENSTFKAELMEKIAWYQLELGKLEALLQQSPQVRTFKKAFEVGFIIYCLHKLI
ITFLKRIPYIIYHSLKYPDDYDYENFSENAASDPLAITIANILDFSFFRFNYQHDLDS
LTKQISLFLSISLFLCCLSAVNTTISYVVTLLPIKFQILALFAMQNDDTANVLPEYTN
NSSYKGKKRNYSQEQKGISLIKNLVVSELTGVYVLATTLMVRSHLPFEVSQRLKELLG
GKFTVPNIVIDSWFDEVYAFACVFTFICIRIAERKLSTKKVSVE"
gene complement(<258244..>261591)
/gene="IRE1"
/locus_tag="YHR079C"
/gene_synonym="ERN1"
/db_xref="GeneID:856478"
mRNA complement(<258244..>261591)
/gene="IRE1"
/locus_tag="YHR079C"
/gene_synonym="ERN1"
/product="bifunctional endoribonuclease/protein kinase
IRE1"
/transcript_id="NM_001179209.1"
/db_xref="GeneID:856478"
CDS complement(258244..261591)
/gene="IRE1"
/locus_tag="YHR079C"
/gene_synonym="ERN1"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004521 RNA
endonuclease activity [PMID:9323131]"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0004674 protein
serine/threonine kinase activity [PMID:8663458]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:17035634]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:8358794]"
/experiment="EXISTENCE:direct assay:GO:0051082 unfolded
protein binding [PMID:17923530]"
/experiment="EXISTENCE:genetic interaction:GO:0031505
fungal-type cell wall organization [PMID:18971375]"
/experiment="EXISTENCE:mutant phenotype:GO:0006020
inositol metabolic process [PMID:1625574]"
/experiment="EXISTENCE:mutant phenotype:GO:0030968
endoplasmic reticulum unfolded protein response
[PMID:8513503|PMID:8358794]"
/experiment="EXISTENCE:mutant phenotype:GO:0031505
fungal-type cell wall organization
[PMID:8898193|PMID:18971375]"
/experiment="EXISTENCE:mutant phenotype:GO:0034067 protein
localization to Golgi apparatus [PMID:26966233]"
/experiment="EXISTENCE:mutant phenotype:GO:0034976
response to endoplasmic reticulum stress [PMID:26966233]"
/experiment="EXISTENCE:mutant phenotype:GO:0051082
unfolded protein binding [PMID:17923530]"
/note="Serine-threonine kinase and endoribonuclease;
transmembrane protein that mediates the unfolded protein
response (UPR) by regulating Hac1p synthesis through HAC1
mRNA splicing; role in homeostatic adaptation to ER
stress; Kar2p binds inactive Ire1p and releases from it
upon ER stress; involved in UPR-mediated suppression of
aneuploidy"
/codon_start=1
/product="bifunctional endoribonuclease/protein kinase
IRE1"
/protein_id="NP_011946.1"
/db_xref="GeneID:856478"
/db_xref="SGD:S000001121"
/translation="MRLLRRNMLVLTLLVCVFSSIISCSIPLSSRTSRRQIVEDEVAS
TKKLNFNYGVDKNINSPIPAPRTTEGLPNMKLSSYPTPNLLNTADNRRANKKGRRAAN
SISVPYLENRSLNELSLSDILIAADVEGGLHAVDRRNGHIIWSIEPENFQPLIEIQEP
SRLETYETLIIEPFGDGNIYYFNAHQGLQKLPLSIRQLVSTSPLHLKTNIVVNDSGKI
VEDEKVYTGSMRTIMYTINMLNGEIISAFGPGSKNGYFGSQSVDCSPEEKIKLQECEN
MIVIGKTIFELGIHSYDGASYNVTYSTWQQNVLDVPLALQNTFSKDGMCIAPFRDKSL
LASDLDFRIARWVSPTFPGIIVGLFDVFNDLRTNENILVPHPFNPGDHESISSNKVYL
DQTSNLSWFALSSQNFPSLVESAPISRYASSDRWRVSSIFEDETLFKNAIMGVHQIYN
NEYDHLYENYEKTNSLDTTHKYPPLMIDSSVDTTDLHQNNEMNSLKEYMSPEDLEAYR
KKIHEQISRELDEKNQNSLLLKFGSLVYRIIETGVFLLLFLIFCAILQRFKILPPLYV
LLSKIGFMPEKEIPIVESKSLNCPSSSENVTKPFDMKSGKQVVFEGAVNDGSLKSEKD
NDDADEDDEKSLDLTTEKKKRKRGSRGGKKGRKSRIANIPNFEQSLKNLVVSEKILGY
GSSGTVVFQGSFQGRPVAVKRMLIDFCDIALMEIKLLTESDDHPNVIRYYCSETTDRF
LYIALELCNLNLQDLVESKNVSDENLKLQKEYNPISLLRQIASGVAHLHSLKIIHRDL
KPQNILVSTSSRFTADQQTGAENLRILISDFGLCKKLDSGQSSFRTNLNNPSGTSGWR
APELLEESNNLQCQVETEHSSSRHTVVSSDSFYDPFTKRRLTRSIDIFSMGCVFYYIL
SKGKHPFGDKYSRESNIIRGIFSLDEMKCLHDRSLIAEATDLISQMIDHDPLKRPTAM
KVLRHPLFWPKSKKLEFLLKVSDRLEIENRDPPSALLMKFDAGSDFVIPSGDWTVKFD
KTFMDNLERYRKYHSSKLMDLLRALRNKYHHFMDLPEDIAELMGPVPDGFYDYFTKRF
PNLLIGVYMIVKENLSDDQILREFLYS"
gene complement(<262192..>262553)
/gene="SAE3"
/locus_tag="YHR079C-A"
/gene_synonym="YHR079C-B"
/db_xref="GeneID:856479"
mRNA complement(join(<262192..262354,262441..>262553))
/gene="SAE3"
/locus_tag="YHR079C-A"
/gene_synonym="YHR079C-B"
/product="Sae3p"
/transcript_id="NM_001180026.1"
/db_xref="GeneID:856479"
CDS complement(join(262192..262354,262441..262553))
/gene="SAE3"
/locus_tag="YHR079C-A"
/gene_synonym="YHR079C-B"
/experiment="EXISTENCE:direct assay:GO:0000794 condensed
nuclear chromosome [PMID:15579681|PMID:15620352]"
/experiment="EXISTENCE:direct assay:GO:0030234 enzyme
regulator activity [PMID:39275989]"
/experiment="EXISTENCE:mutant phenotype:GO:0000707 meiotic
DNA recombinase assembly [PMID:15579681|PMID:15620352]"
/experiment="EXISTENCE:mutant phenotype:GO:0007131
reciprocal meiotic recombination [PMID:9215889]"
/note="Meiosis-specific protein involved in meiotic
recombination; involved in DMC1-dependent meiotic
recombination; forms heterodimer with Mei5p; proposed to
be an assembly factor for Dmc1p"
/codon_start=1
/product="Sae3p"
/protein_id="NP_011947.2"
/db_xref="GeneID:856479"
/db_xref="SGD:S000001957"
/translation="MNYLETQLNKKQKQIQEYESMNGNLIKMFEQLSKEKKNDETPKK
ISSTYIKELKEYNELRDAGLRLAQIIADEKQCKIKDVFEEIGYSMKD"
gene complement(<262801..>266838)
/gene="LAM4"
/locus_tag="YHR080C"
/gene_synonym="LTC3"
/db_xref="GeneID:856480"
mRNA complement(<262801..>266838)
/gene="LAM4"
/locus_tag="YHR080C"
/gene_synonym="LTC3"
/product="Lam4p"
/transcript_id="NM_001179210.1"
/db_xref="GeneID:856480"
CDS complement(262801..266838)
/gene="LAM4"
/locus_tag="YHR080C"
/gene_synonym="LTC3"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0032541 cortical
endoplasmic reticulum [PMID:26001273]"
/experiment="EXISTENCE:direct assay:GO:0032934 sterol
binding [PMID:26001273]"
/experiment="EXISTENCE:direct assay:GO:0120015 sterol
transfer activity [PMID:29339490]"
/experiment="EXISTENCE:mutant phenotype:GO:0015918 sterol
transport [PMID:33255682]"
/note="Sterol-binding protein that localizes to puncta in
the cortical ER; sterol binding occurs via two StART-like
domains; one of six StART-like domain-containing proteins
in yeast that may be involved in intracellular sterol
transfer between membranes; conserved across eukaryotes;
has both GRAM and StART-like (VASt) domains; the
authentic, non-tagged protein is detected in highly
purified mitochondria in high-throughput studies"
/codon_start=1
/product="Lam4p"
/protein_id="NP_011948.1"
/db_xref="GeneID:856480"
/db_xref="SGD:S000001122"
/translation="MTRDSKKKHHWGTAFLRTIGVKRKHKKDRNFLNNTTGENVSTTA
SAERFRRVGGNPDIPSLLKPETFTESPAKGSQKAAASSLAHSQGVFNIPIVIDPMETN
RLEKTNTNLTAGSLKGRFQDGNSNSNSVPSLSVQALEKEKLQSGKREGSSNQAEEKTP
DGHDEHTAFETFLSFAHNAVSHIPKINVQDADNGTISRNEPKDRKKNSSNISGALSEN
STNNKNTSSTKESDGPFLKNLDNILAASKSSTPSNQQLNTTEAGSKSKPSSLSRLAFG
NLKGHIHSNSHSSSNAISGDDTSLDDTRKMTDDMARKVVFEPIRHSHDKPTPGVGNLK
LEHFDDSQATLEGLEAMSAESLPEADHLDSRGPVQQSNLERKTVPSKWSVVSSSTTDG
VKPRRRAKSMISAMADKQNTSSDVLQDCKKRLSFNSSNGLTNNDPEYEDREPREMSKK
FLNRRSFSPGSISMGMKVLPSTALKYSLNKVKNSTDIASTIIPRPSMSNGRPSSGLRR
SSSKSFSSTPVNIIEPSEENGRQSSIRIKGVEYASEKKDAEFHAIFKDSGVSPNERLI
LDHSCALSRDILLQGRMYISDQHIGFYSNILGWVSTVFIPFKTIVQIEKRATAGIFPN
GIVIDTLHTKYTFASFTSRDATYDLITEVWNQIILGKRFRSNSNNTNSSSNSISDDEN
DDYDDDYDDYGDDDDDLYDNSNNISDSTDMTSSVSIGKPEDLPMPLQTDTPYGTGIPP
LGPKIHSPTETVYKPAPNEKLVNESTIHASLGRVVNILFGKDVSYIMAILKAQKNSDI
SPIPVLVDSPTVSEGKKRDYSYVKTTPGAIGPGKTKCMITETIQHFNLEEYVQVLQTT
KTPDVPSGNSFYVRTVYLLSWANNNETKLKLYVSVEWTGKSLIKSPIEKGTFDGVTDA
TKILVEELGNILTRSATKRKRSSKENTVTVSTLPKMEPSSHAPTEPDIQKDKDDSIIR
ENENIPAPLGTVVQLLFGSNTEYMQKVITRDKNNVNVETIPKFTPSLVEGGSRHYEYT
KKLNNSIGPKQTKCLLTESIEHMDINNYVLVTQTTKTPDVPSGSNFAVESKIFLFWGQ
HDTTNMTVITKINWTSKSFLKGAIEKGSVEGQKVSVDYMLSELRDIISRAKSKKPVKK
VMKSHDKHRPFHSKVEQKSSESRKSDDNKDILTHILDFVQNNFSSEIFMNKLLSPQKL
FLILGLTIMLFWSPRLHVFQEKNNLQIIKPGRLLIDGQEYNYVPSFGTLYNSYENAIS
SKKKRENVNYARDKSPIVGRESDIWDWISNRGSAISPRGRAMLRNDDEHKLQQLSESI
KITEMQLNHMKTMLDNIERDANDLS"
gene <267538..>268092
/gene="LRP1"
/locus_tag="YHR081W"
/gene_synonym="RRP47"
/db_xref="GeneID:856481"
mRNA <267538..>268092
/gene="LRP1"
/locus_tag="YHR081W"
/gene_synonym="RRP47"
/product="Lrp1p"
/transcript_id="NM_001179211.1"
/db_xref="GeneID:856481"
CDS 267538..268092
/gene="LRP1"
/locus_tag="YHR081W"
/gene_synonym="RRP47"
/experiment="EXISTENCE:direct assay:GO:0003690
double-stranded DNA binding [PMID:17704127]"
/experiment="EXISTENCE:direct assay:GO:0003725
double-stranded RNA binding [PMID:17704127]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:23580640]"
/experiment="EXISTENCE:direct assay:GO:0030234 enzyme
regulator activity [PMID:24937447]"
/experiment="EXISTENCE:direct assay:GO:0044877
protein-containing complex binding
[PMID:19046973|PMID:12837249]"
/experiment="EXISTENCE:genetic interaction:GO:0071028
nuclear mRNA surveillance [PMID:17410208]"
/experiment="EXISTENCE:genetic interaction:GO:0071035
nuclear polyadenylation-dependent rRNA catabolic process
[PMID:18591258]"
/experiment="EXISTENCE:genetic interaction:GO:0071039
nuclear polyadenylation-dependent CUT catabolic process
[PMID:18591258]"
/experiment="EXISTENCE:mutant phenotype:GO:0000467
exonucleolytic trimming to generate mature 3'-end of 5.8S
rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S
rRNA, LSU-rRNA) [PMID:12972615]"
/experiment="EXISTENCE:mutant phenotype:GO:0000973
post-transcriptional tethering of RNA polymerase II gene
DNA at nuclear periphery [PMID:18614049]"
/experiment="EXISTENCE:mutant phenotype:GO:0034475 U4
snRNA 3'-end processing [PMID:12972615]"
/experiment="EXISTENCE:mutant phenotype:GO:0034476 U5
snRNA 3'-end processing [PMID:12972615]"
/experiment="EXISTENCE:mutant phenotype:GO:0071028 nuclear
mRNA surveillance [PMID:16652390]"
/experiment="EXISTENCE:mutant phenotype:GO:0071035 nuclear
polyadenylation-dependent rRNA catabolic process
[PMID:12972615]"
/experiment="EXISTENCE:mutant phenotype:GO:0071039 nuclear
polyadenylation-dependent CUT catabolic process
[PMID:16973436|PMID:18591258]"
/experiment="EXISTENCE:mutant phenotype:GO:0071051
poly(A)-dependent snoRNA 3'-end processing
[PMID:12972615]"
/note="Nuclear exosome-associated nucleic acid binding
protein; involved in RNA processing, surveillance,
degradation, tethering, and export; forms a stable
heterodimer with Rrp6p and regulates its exonucleolytic
activity; rapidly degraded by the proteasome in the
absence of Rrp6p; homolog of mammalian nuclear matrix
protein C1D involved in regulation of DNA repair and
recombination"
/codon_start=1
/product="Lrp1p"
/protein_id="NP_011949.1"
/db_xref="GeneID:856481"
/db_xref="SGD:S000001123"
/translation="MEDIEKIKPYVRSFSKALDELKPEIEKLTSKSLDEQLLLLSDER
AKLELINRYAYVLSSLMFANMKVLGVKDMSPILGELKRVKSYMDKAKQYDNRITKSNE
KSQAEQEKAKNIISNVLDGNKNQFEPSISRSNFQGKHTKFENDELAESTTTKIIDSTD
HIRKASSKKSKRLDKVGKKKGGKK"
gene complement(<268459..>271548)
/gene="KSP1"
/locus_tag="YHR082C"
/db_xref="GeneID:856482"
mRNA complement(<268459..>271548)
/gene="KSP1"
/locus_tag="YHR082C"
/product="putative serine/threonine protein kinase KSP1"
/transcript_id="NM_001179212.1"
/db_xref="GeneID:856482"
CDS complement(268459..271548)
/gene="KSP1"
/locus_tag="YHR082C"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:8676864|PMID:18417610]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:18417610|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:23222640|PMID:26777405]"
/experiment="EXISTENCE:genetic interaction:GO:0016242
negative regulation of macroautophagy [PMID:22447937]"
/experiment="EXISTENCE:mutant phenotype:GO:0008104
intracellular protein localization [PMID:18417610]"
/experiment="EXISTENCE:mutant phenotype:GO:0010570
regulation of filamentous growth [PMID:18417610]"
/experiment="EXISTENCE:mutant phenotype:GO:0016242
negative regulation of macroautophagy [PMID:22447937]"
/experiment="EXISTENCE:mutant phenotype:GO:0031929 TOR
signaling [PMID:22043304]"
/experiment="EXISTENCE:mutant phenotype:GO:0043555
regulation of translation in response to stress
[PMID:31455721]"
/experiment="EXISTENCE:mutant phenotype:GO:2000220
regulation of pseudohyphal growth
[PMID:31455721|PMID:22043304]"
/experiment="EXISTENCE:physical interaction:GO:0031929 TOR
signaling [PMID:22043304]"
/note="Serine/threonine protein kinase; associates with
TORC1 and likely involved in TOR signaling cascades;
negative regulator of autophagy; nuclear translocation
required for haploid filamentous growth; regulates
filamentous growth induced nuclear translocation of Bcy1p,
Fus3p, and Sks1p; overproduction causes allele-specific
suppression of prp20-10; protein abundance increases in
response to DNA replication stress"
/codon_start=1
/product="putative serine/threonine protein kinase KSP1"
/protein_id="NP_011950.1"
/db_xref="GeneID:856482"
/db_xref="SGD:S000001124"
/translation="MTLDYEIYKEGGILNNRYQKIEDISEGSYGYVSLAKDVREKRLV
AVKYIFKLEDDGQYDGPQDDENDCDSSDCDDDEDTKVDTDRHENENGNASSNNGSSRE
KKHNLYKHKKSLISSKVKSRLSNNICLEAMYEVDIQTKIGRHQNIAALLDFFDSYIIM
EYCSGGDLYEAIKADAVPKKTKSITHIITQIMDAIEYVHNKGIYHRDIKPENILISGI
DWTIKLTDWGLATTDKTSMDRNVGSERYMSPELFDSNLDIKERKEPYDCAKVDLWAMG
IVFLNIVFHKNPFSIANQSDKSFCYFAANREALFDVFSTMAYDFFQVLRYSLTIDPAN
RDLKMMRTELQNLSEYTLDDEYYNNLDEGYEETMIDGLPPQPVPPSSAPVSLPTPISS
SNKQHMPEFKKDFNFNNVNERKRSDVSQNQNVASGFFKKPSTQQQKFFNQGYNTTLST
HERAKSAPKFKFKKRNKYGRTDNQFSKPVNIEDRKKSKILKKSRKPLGIPTPNTHMNN
FFHDYKARDEFNTRDFFTPPSVQHRYMEGFSNNNNKQYRQNRNYNNNNNNSNNNHGSN
YNNFNNGNSYIKGWNKNFNKYRRPSSSSYTGKSPLSRYNMSYNHNNNSSINGYARRGS
TTTVQHSPGAYIPPNARNHHVSPTNQFLRVPQSTAPDISTVLGGKPSYQEHYTQDSMD
SEGDHDSDDVLFTLEEGDHDFVNGMDNLSINDHLPHTTVGSHNEVFVHASTNHNNNGN
NNHIDTNSTTNQYHRQYIPPPLTTSLHINNNNNESNELPDLLKSPASSEAHLNLSSGP
IDPILTGNIGNRYSHSSDSKELEQERRLSMEQKFKNGVYVPPHHRKSFNLGTQVPPMN
MKTSNEATLSVSHNSVNFGGSYNSRRSSANESNPLHMNKALEKLSSSPGAKSSFVGFP
KPLLPRNHSSTTIALQNEDVFADSNNDAIIFEDEEYEGESDKMAHGKMEGGDNESSST
SPDERQIFGPYEIYAQTFAGSTHDKKLGAGRKTSIQDEMVGSLEQYKNNWLILQQQD"
gene <272627..>273616
/gene="SAM35"
/locus_tag="YHR083W"
/gene_synonym="FMP20; TOB38; TOM38"
/db_xref="GeneID:856483"
mRNA <272627..>273616
/gene="SAM35"
/locus_tag="YHR083W"
/gene_synonym="FMP20; TOB38; TOM38"
/product="SAM complex subunit SAM35"
/transcript_id="NM_001179213.1"
/db_xref="GeneID:856483"
CDS 272627..273616
/gene="SAM35"
/locus_tag="YHR083W"
/gene_synonym="FMP20; TOB38; TOM38"
/experiment="EXISTENCE:direct assay:GO:0001401 SAM complex
[PMID:15067005|PMID:15205677]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane
[PMID:15205677|PMID:16407407|PMID:15067005]"
/experiment="EXISTENCE:mutant phenotype:GO:0001401 SAM
complex [PMID:15205677]"
/experiment="EXISTENCE:mutant phenotype:GO:0045040 protein
insertion into mitochondrial outer membrane
[PMID:15326197|PMID:18039934|PMID:15067005]"
/experiment="EXISTENCE:mutant phenotype:GO:0070096
mitochondrial outer membrane translocase complex assembly
[PMID:18039934|PMID:15205677|PMID:15067005]"
/note="Component of the sorting and assembly machinery
(SAM) complex; the SAM (or TOB) complex is located in the
mitochondrial outer membrane; the complex binds precursors
of beta-barrel proteins and facilitates their insertion
into the outer membrane"
/codon_start=1
/product="SAM complex subunit SAM35"
/protein_id="NP_011951.1"
/db_xref="GeneID:856483"
/db_xref="SGD:S000001125"
/translation="MVSSFSVPMPVKRIFDTFPLQTYAAQTDKDEAVALEIQRRSYTF
TERGGGSSELTVEGTYKLGVYNVFLEANTGAALATDPWCLFVQLALCQKNGLVLPTHS
QEQTPSHTCNHEMLVLSRLSNPDEALPILVEGYKKRIIRSTVAISEIMRSRILDDAEQ
LMYYTLLDTVLYDCWITQIIFCASDAQFMELYSCQKLSGSIVTPLDVENSLLQKLSAK
SLKISLTKRNKFQFRHREIVKSMQGVYHNHHNSVNQEQVLNVLFENSKQVLLGLKDML
KSDGQPTYLHLKIASYILCITNVKEPIKLKTFVENECKELVQFAQDTLKNFVQ"
gene <274174..>276240
/gene="STE12"
/locus_tag="YHR084W"
/db_xref="GeneID:856484"
mRNA <274174..>276240
/gene="STE12"
/locus_tag="YHR084W"
/product="homeodomain family transcription factor STE12"
/transcript_id="NM_001179214.1"
/db_xref="GeneID:856484"
CDS 274174..276240
/gene="STE12"
/locus_tag="YHR084W"
/experiment="EXISTENCE:direct assay:GO:0000747 conjugation
with cellular fusion [PMID:3931921]"
/experiment="EXISTENCE:direct assay:GO:0003700 DNA-binding
transcription factor activity [PMID:2558054]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:19159085]"
/experiment="EXISTENCE:direct assay:GO:0005654 nucleoplasm
[PMID:20852627]"
/experiment="EXISTENCE:direct assay:GO:1990526
Ste12p-Dig1p-Dig2p complex [PMID:16782869]"
/experiment="EXISTENCE:direct assay:GO:1990527
Tec1p-Ste12p-Dig1p complex [PMID:16782869]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:16940175]"
/experiment="EXISTENCE:mutant phenotype:GO:0001403
invasive growth in response to glucose limitation
[PMID:8001818]"
/experiment="EXISTENCE:mutant phenotype:GO:0007124
pseudohyphal growth [PMID:9055077|PMID:8259520]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:7834739]"
/experiment="EXISTENCE:mutant phenotype:GO:0071444
cellular response to pheromone
[PMID:16940175|PMID:7834739]"
/note="Transcription factor that is activated by a MAPK
signaling cascade; activates genes involved in mating or
pseudohyphal/invasive growth pathways; cooperates with
Tec1p transcription factor to regulate genes specific for
invasive growth"
/codon_start=1
/product="homeodomain family transcription factor STE12"
/protein_id="NP_011952.1"
/db_xref="GeneID:856484"
/db_xref="SGD:S000001126"
/translation="MKVQITNSRTEEILKVQANNENDEVSKATPGEVEESLRLIGDLK
FFLATAPVNWQENQIIRRYYLNSGQGFVSCVFWNNLYYITGTDIVKCCLYRMQKFGRE
VVQKKKFEEGIFSDLRNLKCGIDATLEQPKSEFLSFLFRNMCLKTQKKQKVFFWFSVA
HDKLFADALERDLKRESLNQPSTTKPVNEPALSFSYDSSSDKPLYDQLLQHLDSRRPS
STTKSDNSPPKLESENFKDNELVTVTNQPLLGVGLMDDDAPESPSQINDFIPQKLIIE
PNTLELNGLTEETPHDLPKNTAKGRDEEDFPLDYFPVSVEYPTEENAFDPFPPQAFTP
AAPSMPISYDNVNERDSMPVNSLLNRYPYQLSVAPTFPVPPSSSRQHFMTNRDFYSSN
NNKEKLVSPSDPTSYMKYDEPVMDFDESRPNENCTNAKSHNSGQQTKQHQLYSNNFQQ
SYPNGMVPGYYPKMPYNPMGGDPLLDQAFYGADDFFFPPEGCDNNMLYPQTATSWNVL
PPQAMQPAPTYVGRPYTPNYRSTPGSAMFPYMQSSNSMQWNTAVSPYSSRAPSTTAKN
YPPSTFYSQNINQYPRRRTVGMKSSQGNVPTGNKQSVGKSAKISKPLHIKTSAYQKQY
KINLETKARPSAGDEDSAHPDKNKEISMPTPDSNTLVVQSEEGGAHSLEVDTNRRSDK
NLPDAT"
gene <276764..>277768
/gene="IPI1"
/locus_tag="YHR085W"
/db_xref="GeneID:856485"
mRNA <276764..>277768
/gene="IPI1"
/locus_tag="YHR085W"
/product="Ipi1p"
/transcript_id="NM_001179215.1"
/db_xref="GeneID:856485"
CDS 276764..277768
/gene="IPI1"
/locus_tag="YHR085W"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:22421151]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14690591|PMID:22932476|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005654 nucleoplasm
[PMID:15528184]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0120330 rixosome
complex [PMID:14759368]"
/experiment="EXISTENCE:mutant phenotype:GO:0000027
ribosomal large subunit assembly [PMID:15528184]"
/experiment="EXISTENCE:mutant phenotype:GO:0000463
maturation of LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12089522]"
/experiment="EXISTENCE:mutant phenotype:GO:0006267
pre-replicative complex assembly involved in nuclear cell
cycle DNA replication [PMID:22421151]"
/experiment="EXISTENCE:mutant phenotype:GO:0006364 rRNA
processing [PMID:14759368]"
/experiment="EXISTENCE:mutant phenotype:GO:0030174
regulation of DNA-templated DNA replication initiation
[PMID:22421151]"
/note="Component of the Rix1 complex and possibly
pre-replicative complexes; required for processing of ITS2
sequences from 35S pre-rRNA; component of the pre-60S
ribosomal particle with the dynein-related AAA-type ATPase
Mdn1p; required for pre-replicative complex (pre-RC)
formation and maintenance during DNA replication
licensing; relocalizes to the cytosol in response to
hypoxia; essential gene"
/codon_start=1
/product="Ipi1p"
/protein_id="NP_011953.1"
/db_xref="GeneID:856485"
/db_xref="SGD:S000001127"
/translation="MTKSRKQKQKKQDFLRKKLKVGKPKEKARNATDTSFVSKTISIR
NQHLDQNPHDLTKRLTLLKHHNINVRKETLTTFQKSIPSIIKSRLMTPLLTQSIPLIC
DESQQVRQGLIDLVDEIGSHDAEILKLHCNIFVLYINMAMTHIVTQIQADSTKFLSHL
LKYCGDEVVRKSWVKLLNGVFGVLGWGQVGKNDSASIVQTKKRNAKYVTIHLNALYTL
VEYGCQDERARSDGDTAETTEDSGTLRNPYLIPDYPQPFEHLKLFTRELKVQDATSSG
VNATLLSLATQDIDTRKAVFIEQFLPIVRKKIEVIIKEGGECGKSANKLKTLLAKIFD
"
gene <278153..>279724
/gene="NAM8"
/locus_tag="YHR086W"
/gene_synonym="MRE2; MUD15"
/db_xref="GeneID:856486"
mRNA <278153..>279724
/gene="NAM8"
/locus_tag="YHR086W"
/gene_synonym="MRE2; MUD15"
/product="Nam8p"
/transcript_id="NM_001179216.1"
/db_xref="GeneID:856486"
CDS 278153..279724
/gene="NAM8"
/locus_tag="YHR086W"
/gene_synonym="MRE2; MUD15"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005685 U1 snRNP
[PMID:9630245]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0071004 U2-type
prespliceosome [PMID:16618970]"
/experiment="EXISTENCE:mutant phenotype:GO:0006376 mRNA
splice site recognition [PMID:10072385]"
/experiment="EXISTENCE:mutant phenotype:GO:0048026
positive regulation of mRNA splicing, via spliceosome
[PMID:10983980]"
/experiment="EXISTENCE:physical interaction:GO:0000243
commitment complex [PMID:10072386]"
/experiment="EXISTENCE:physical interaction:GO:0000398
mRNA splicing, via spliceosome [PMID:10072386]"
/experiment="EXISTENCE:physical interaction:GO:0003729
mRNA binding [PMID:10072386]"
/note="RNA binding protein, component of the U1 snRNP
protein; mutants are defective in meiotic recombination
and in formation of viable spores, involved in the
formation of DSBs through meiosis-specific splicing of
REC107 pre-mRNA; Nam8p regulon embraces the meiotic
pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the
putative RNA binding domains RRM2 and RRM3 are required
for Nam8p meiotic function"
/codon_start=1
/product="Nam8p"
/protein_id="NP_011954.1"
/db_xref="GeneID:856486"
/db_xref="SGD:S000001128"
/translation="MSYKQTTYYPSRGNLVRNDSSPYTNTISSETNNSSTSVLSLQGA
SNVSLGTTGNQLYMGDLDPTWDKNTVRQIWASLGEANINVRMMWNNTLNNGSRSSMGP
KNNQGYCFVDFPSSTHAANALLKNGMLIPNFPNKKLKLNWATSSYSNSNNSLNNVKSG
NNCSIFVGDLAPNVTESQLFELFINRYASTSHAKIVHDQVTGMSKGYGFVKFTNSDEQ
QLALSEMQGVFLNGRAIKVGPTSGQQQHVSGNNDYNRSSSSLNNENVDSRFLSKGQSF
LSNGNNNMGFKRNHMSQFIYPVQQQPSLNHFTDPNNTTVFIGGLSSLVTEDELRAYFQ
PFGTIVYVKIPVGKCCGFVQYVDRLSAEAAIAGMQGFPIANSRVRLSWGRSAKQTALL
QQAMLSNSLQVQQQQPGLQQPNYGYIPSSTCEAPVLPDNNVSSTMLPGCQILNYSNPY
ANANGLGSNNFSFYSNNNATNTQATSLLADTSSMDLSGTGGQQVIMQGSEAVVNSTNA
MLNRLEQGSNGFMFA"
gene <280231..>280392
/locus_tag="YHR086W-A"
/db_xref="GeneID:1466529"
mRNA <280231..>280392
/locus_tag="YHR086W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184553.1"
/db_xref="GeneID:1466529"
CDS 280231..280392
/locus_tag="YHR086W-A"
/note="hypothetical protein; identified by fungal homology
and RT-PCR; SWAT-GFP and mCherry fusion proteins localize
to the endoplasmic reticulum and vacuole respectively"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878089.1"
/db_xref="GeneID:1466529"
/db_xref="SGD:S000028552"
/translation="MRLSLYSCCLCIGARRIPTPCLFSYIFTPNLAHFHSPLRSSGFR
PAGATRFYS"
gene <280820..>281155
/gene="RTC3"
/locus_tag="YHR087W"
/gene_synonym="HGI1"
/db_xref="GeneID:856487"
mRNA <280820..>281155
/gene="RTC3"
/locus_tag="YHR087W"
/gene_synonym="HGI1"
/product="Rtc3p"
/transcript_id="NM_001179217.1"
/db_xref="GeneID:856487"
CDS 280820..281155
/gene="RTC3"
/locus_tag="YHR087W"
/gene_synonym="HGI1"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0016070 RNA
metabolic process [PMID:15701634]"
/note="hypothetical protein involved in RNA metabolism;
has structural similarity to SBDS, the human protein
mutated in Shwachman-Diamond Syndrome (the yeast SBDS
ortholog is SDO1); null mutation suppresses cdc13-1
temperature sensitivity; protein abundance increases in
response to DNA replication stress"
/codon_start=1
/product="Rtc3p"
/protein_id="NP_011955.1"
/db_xref="GeneID:856487"
/db_xref="SGD:S000001129"
/translation="MSTVTKYFYKGENTDLIVFAASEELVDEYLKNPSIGKLSEVVEL
FEVFTPQDGRGAEGELGAASKAQVENEFGKGKKIEEVIDLILRNGKPNSTTSSLKTKG
GNAGTKAYN"
gene <281495..>282382
/gene="RPF1"
/locus_tag="YHR088W"
/db_xref="GeneID:856488"
mRNA <281495..>282382
/gene="RPF1"
/locus_tag="YHR088W"
/product="rRNA-binding ribosome biosynthesis protein RPF1"
/transcript_id="NM_001179218.1"
/db_xref="GeneID:856488"
CDS 281495..282382
/gene="RPF1"
/locus_tag="YHR088W"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:11864606]"
/experiment="EXISTENCE:direct assay:GO:0030687
preribosome, large subunit precursor
[PMID:11583614|PMID:17443350]"
/experiment="EXISTENCE:direct assay:GO:0042134 rRNA
primary transcript binding [PMID:11864606]"
/experiment="EXISTENCE:mutant phenotype:GO:0000055
ribosomal large subunit export from nucleus
[PMID:16581791]"
/experiment="EXISTENCE:mutant phenotype:GO:0000463
maturation of LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11864606]"
/experiment="EXISTENCE:mutant phenotype:GO:0000466
maturation of 5.8S rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11864606]"
/note="Protein involved in assembly and export of the
large ribosomal subunit; nucleolar protein; constituent of
66S pre-ribosomal particles; contains a sigma(70)-like
motif, which is thought to bind RNA"
/codon_start=1
/product="rRNA-binding ribosome biosynthesis protein RPF1"
/protein_id="NP_011956.1"
/db_xref="GeneID:856488"
/db_xref="SGD:S000001130"
/translation="MALGNEINITNKLKRQEIFADIKHEKNKERHTMRRKRAKEEREN
PELREQRLKENVTQTIENTRVYDETINKEVEGDEDDLMRYFNSNSNEPPKIFLTTNVN
AKKSAYEFANILIEILPNVTFVKRKFGYKLKEISDICIKRNFTDIVIINEDKKKVTGL
TFIHLPEGPTFYFKLSSFVEVKKIVGHGRPTSHIPELILNNFQTRLGQTVGRLFQSIL
PQNPDIEGRQVITLHNQRDYIFFRRHRYVFKDNERVGLQELGPQFTLKLKRLQRGIKE
ETEWEHKPEMDKEKKKFYL"
gene complement(<282681..>283298)
/gene="GAR1"
/locus_tag="YHR089C"
/db_xref="GeneID:856489"
mRNA complement(<282681..>283298)
/gene="GAR1"
/locus_tag="YHR089C"
/product="H/ACA snoRNP pseudouridylase subunit GAR1"
/transcript_id="NM_001179219.1"
/db_xref="GeneID:856489"
CDS complement(282681..283298)
/gene="GAR1"
/locus_tag="YHR089C"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:1531632]"
/experiment="EXISTENCE:direct assay:GO:0031120 snRNA
pseudouridine synthesis [PMID:15962000]"
/experiment="EXISTENCE:physical interaction:GO:0031429 box
H/ACA snoRNP complex [PMID:2898766]"
/experiment="EXISTENCE:physical interaction:GO:0034513 box
H/ACA snoRNA binding [PMID:9556561]"
/note="Protein component of the H/ACA snoRNP
pseudouridylase complex; involved in the modification and
cleavage of the 18S pre-rRNA"
/codon_start=1
/product="H/ACA snoRNP pseudouridylase subunit GAR1"
/protein_id="NP_011957.1"
/db_xref="GeneID:856489"
/db_xref="SGD:S000001131"
/translation="MSFRGGNRGGRGGFRGGFRGGRTGSARSFQQGPPDTVLEMGAFL
HPCEGDIVCRSINTKIPYFNAPIYLENKTQVGKVDEILGPLNEVFFTIKCGDGVQATS
FKEGDKFYIAADKLLPIERFLPKPKVVGPPKPKNKKKRSGAPGGRGGASMGRGGSRGG
FRGGRGGSSFRGGRGGSSFRGGSRGGSFRGGSRGGSRGGFRGGRR"
gene complement(<283777..>284625)
/gene="YNG2"
/locus_tag="YHR090C"
/gene_synonym="EAF4; NBN1"
/db_xref="GeneID:856490"
mRNA complement(<283777..>284625)
/gene="YNG2"
/locus_tag="YHR090C"
/gene_synonym="EAF4; NBN1"
/product="histone acetyltransferase YNG2"
/transcript_id="NM_001179220.1"
/db_xref="GeneID:856490"
CDS complement(283777..284625)
/gene="YNG2"
/locus_tag="YHR090C"
/gene_synonym="EAF4; NBN1"
/experiment="EXISTENCE:direct assay:GO:0004402 histone
acetyltransferase activity [PMID:17274630]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476|PMID:10805724]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0006281 DNA repair
[PMID:16135807]"
/experiment="EXISTENCE:direct assay:GO:0032777 piccolo
histone acetyltransferase complex [PMID:12782659]"
/experiment="EXISTENCE:direct assay:GO:0035267 NuA4
histone acetyltransferase complex [PMID:15485911]"
/experiment="EXISTENCE:direct assay:GO:0140002 histone
H3K4me3 reader activity [PMID:17142463]"
/note="Subunit of NuA4, an essential histone
acetyltransferase complex; positions Piccolo NuA4 for
efficient acetylation of histone H4 or histone H2A;
relocalizes to the cytosol in response to hypoxia; similar
to human tumor suppressor ING1 and its isoforms ING4 and
ING5"
/codon_start=1
/product="histone acetyltransferase YNG2"
/protein_id="NP_011958.1"
/db_xref="GeneID:856490"
/db_xref="SGD:S000001132"
/translation="MDPSLVLEQTIQDVSNLPSEFRYLLEEIGSNDLKLIEEKKKYEQ
KESQIHKFIRQQGSIPKHPQEDGLDKEIKESLLKCQSLQREKCVLANTALFLIARHLN
KLEKNIALLEEDGVLAPVEEDGDMDSAAEASRESSVVSNSSVKKRRAASSSGSVPPTL
KKKKTSRTSKLQNEIDVSSREKSVTPVSPSIEKKIARTKEFKNSRNGKGQNGSPENEE
EDKTLYCFCQRVSFGEMVACDGPNCKYEWFHYDCVNLKEPPKGTWYCPECKIEMEKNK
LKRKRN"
gene complement(<284839..>286770)
/gene="MSR1"
/locus_tag="YHR091C"
/db_xref="GeneID:856491"
mRNA complement(<284839..>286770)
/gene="MSR1"
/locus_tag="YHR091C"
/product="arginine--tRNA ligase MSR1"
/transcript_id="NM_001179221.1"
/db_xref="GeneID:856491"
CDS complement(284839..286770)
/gene="MSR1"
/locus_tag="YHR091C"
/EC_number="6.1.1.19"
/experiment="EXISTENCE:curator inference:GO:0070144
mitochondrial arginyl-tRNA aminoacylation [PMID:7607232]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:mutant phenotype:GO:0005739
mitochondrion [PMID:7607232]"
/experiment="EXISTENCE:mutant phenotype:GO:0032543
mitochondrial translation [PMID:7607232]"
/note="Mitochondrial arginyl-tRNA synthetase; mutations in
human ortholog are associated with pontocerebellar
hypoplasia type 6; MSR1 has a paralog, YDR341C, that arose
from the whole genome duplication"
/codon_start=1
/product="arginine--tRNA ligase MSR1"
/protein_id="NP_011959.1"
/db_xref="GeneID:856491"
/db_xref="SGD:S000001133"
/translation="MFGIVYLKNRSLLCKNPFSSYPRYGFMPSFDTQFSNQFRKLEIN
IGRKRYSSKTLNTKYTDQPEGPIYPLDVLRLDISKALHDISGIDHSLILNALESTNSM
DRGDLLLPLPKIKVADPVAVANRWAIELSTHGCIGKVCAKGPFLQFFLDQRYLIQSTV
PNILLQKGKYGQKKSRHQKKVVVEFSSPNIAKPFHAGHLRSTIIGGFLSNLYEAMGWS
VTRMNYLGDWGRQFGLLAVGFKRYGDEKTLQKQPIQHLFDVYVKINMDLAKEEINGNS
KCGISGEARSFFKNLENGDENAIKIWNRFRSLSIHHYIQTYSRLNINFDIFSGESQVS
KESMNEALDIFRKNNLVKEIDGALVIDLTQWSKRLGRVVVQKSDGTTLYLTRDVGAAI
ERKKNLHFDKMVYVISSQQDLYMSQFFMILKKMNFEWAKDLQHINFGMVQGMSTRKGN
VVFLDTILDEARDKALQIMENNKMKISQVDNPQRVADLIGVSAIIIQDMKSKRINNYE
FNWNRMLSFEGDTGPYLQYTHSRLRSLERTSSDFTTDMLIHADFSNLNEPQLVELVRL
LAQYPDVLRRAFETQEPATIVTYLFKVCHQVSSCYKKIWVSGKPADIAIPRLAVYSAS
RQVLHNAMSLLGLVPVDRM"
rep_origin 286770..287128
/note="ARS814; Putative replication origin; identified in
multiple array studies, not yet confirmed by plasmid-based
assay"
/db_xref="SGD:S000130149"
gene complement(<287081..>288811)
/gene="HXT4"
/locus_tag="YHR092C"
/gene_synonym="LGT1; RAG1"
/db_xref="GeneID:856492"
mRNA complement(<287081..>288811)
/gene="HXT4"
/locus_tag="YHR092C"
/gene_synonym="LGT1; RAG1"
/product="hexose transporter HXT4"
/transcript_id="NM_001179222.2"
/db_xref="GeneID:856492"
CDS complement(287081..288811)
/gene="HXT4"
/locus_tag="YHR092C"
/gene_synonym="LGT1; RAG1"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:24124599|PMID:17507646]"
/experiment="EXISTENCE:direct assay:GO:0055056 D-glucose
transmembrane transporter activity [PMID:12702270]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0015146 pentose
transmembrane transporter activity [PMID:17180689]"
/note="High-affinity glucose transporter; member of the
major facilitator superfamily, expression is induced by
low levels of glucose and repressed by high levels of
glucose; HXT4 has a paralog, HXT7, that arose from the
whole genome duplication"
/codon_start=1
/product="hexose transporter HXT4"
/protein_id="NP_011960.2"
/db_xref="GeneID:856492"
/db_xref="SGD:S000001134"
/translation="MSEEAAYQEDTAVQNTPADALSPVESDSNSALSTPSNKAERDDM
KDFDENHEESNNYVEIPKKPASAYVTVSICCLMVAFGGFVFGWDTGTISGFVAQTDFI
RRFGMKHHDGTYYLSKVRTGLIVSIFNIGCAIGGIILAKLGDMYGRKMGLIVVVVIYI
IGIIIQIASINKWYQYFIGRIISGLGVGGIAVLSPMLISEVSPKHIRGTLVSCYQLMI
TLGIFLGYCTNYGTKTYTNSVQWRVPLGLGFAWALFMIGGMTFVPESPRYLVEVGKIE
EAKRSIALSNKVSADDPAVMAEVEVVQATVEAEKLAGNASWGEIFSTKTKVFQRLIMG
AMIQSLQQLTGDNYFFYYGTTVFTAVGLEDSFETSIVLGIVNFASTFVGIFLVERYGR
RRCLLWGAASMTACMVVFASVGVTRLWPNGKKNGSSKGAGNCMIVFTCFYLFCFATTW
APIPFVVNSETFPLRVKSKCMAIAQACNWIWGFLIGFFTPFISGAIDFYYGYVFMGCL
VFSYFYVFFFVPETKGLTLEEVNTLWEEGVLPWKSPSWVPPNKRGTDYNADDLMHDDQ
PFYKKMFGKK"
gene <289142..>289690
/gene="AHT1"
/locus_tag="YHR093W"
/db_xref="GeneID:856493"
mRNA <289142..>289690
/gene="AHT1"
/locus_tag="YHR093W"
/product="Aht1p"
/transcript_id="NM_001348818.1"
/db_xref="GeneID:856493"
CDS 289142..289690
/gene="AHT1"
/locus_tag="YHR093W"
/note="hypothetical protein; conserved across S.
cerevisiae strains; not conserved in closely related
Saccharomyces species; multicopy suppressor of glucose
transport defects, likely due to the presence of an HXT4
regulatory element in the region"
/codon_start=1
/product="Aht1p"
/protein_id="NP_001335739.1"
/db_xref="GeneID:856493"
/db_xref="SGD:S000001135"
/translation="MDCKIKAAGKNSGIFHEGGTKSSKSFLTVFIRSVFPLSPSFPAG
GGIWGPMEKKPGGVGKKKGSEKKTAQGNIFFSTERDAGQEKCGILYKHCFSILYGFFW
KKADKPKEKTGNGSGLGIVFPIGQKKIPEPADSDIFLPCFRYAAASDFTKAKRFLVEI
TAVYWVSLEAQPSSASCLFILI"
gene complement(<290913..>292625)
/gene="HXT1"
/locus_tag="YHR094C"
/gene_synonym="HOR4"
/db_xref="GeneID:856494"
mRNA complement(<290913..>292625)
/gene="HXT1"
/locus_tag="YHR094C"
/gene_synonym="HOR4"
/product="hexose transporter HXT1"
/transcript_id="NM_001179224.1"
/db_xref="GeneID:856494"
CDS complement(290913..292625)
/gene="HXT1"
/locus_tag="YHR094C"
/gene_synonym="HOR4"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:10377429|PMID:24124599]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0005353
fructose transmembrane transporter activity
[PMID:7651133]"
/experiment="EXISTENCE:mutant phenotype:GO:0015146 pentose
transmembrane transporter activity [PMID:17180689]"
/experiment="EXISTENCE:mutant phenotype:GO:0015761 mannose
transmembrane transport [PMID:2046678]"
/experiment="EXISTENCE:mutant phenotype:GO:1904659
D-glucose transmembrane transport [PMID:2046678]"
/note="Low-affinity glucose transporter of the major
facilitator superfamily; expression is induced by Hxk2p in
the presence of glucose and repressed by Rgt1p when
glucose is limiting; HXT1 has a paralog, HXT6, what arose
from the whole genome duplication"
/codon_start=1
/product="hexose transporter HXT1"
/protein_id="NP_011962.1"
/db_xref="GeneID:856494"
/db_xref="SGD:S000001136"
/translation="MNSTPDLISPQKSNSSNSYELESGRSKAMNTPEGKNESFHDNLS
ESQVQPAVAPPNTGKGVYVTVSICCVMVAFGGFIFGWDTGTISGFVAQTDFLRRFGMK
HHDGSHYLSKVRTGLIVSIFNIGCAIGGIVLAKLGDMYGRRIGLIVVVVIYTIGIIIQ
IASINKWYQYFIGRIISGLGVGGITVLSPMLISEVAPSEMRGTLVSCYQVMITLGIFL
GYCTNFGTKNYSNSVQWRVPLGLCFAWALFMIGGMMFVPESPRYLVEAGRIDEARASL
AKVNKCPPDHPYIQYELETIEASVEEMRAAGTASWGELFTGKPAMFQRTMMGIMIQSL
QQLTGDNYFFYYGTIVFQAVGLSDSFETSIVFGVVNFFSTCCSLYTVDRFGRRNCLMW
GAVGMVCCYVVYASVGVTRLWPNGQDQPSSKGAGNCMIVFACFYIFCFATTWAPIAYV
VISECFPLRVKSKCMSIASAANWIWGFLISFFTPFITGAINFYYGYVFMGCMVFAYFY
VFFFVPETKGLSLEEVNDMYAEGVLPWKSASWVPVSKRGADYNADDLMHDDQPFYKSL
FSRK"
gene complement(<294669..>296447)
/gene="HXT5"
/locus_tag="YHR096C"
/db_xref="GeneID:856496"
mRNA complement(<294669..>296447)
/gene="HXT5"
/locus_tag="YHR096C"
/product="hexose transporter HXT5"
/transcript_id="NM_001179226.1"
/db_xref="GeneID:856496"
CDS complement(294669..296447)
/gene="HXT5"
/locus_tag="YHR096C"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836]"
/experiment="EXISTENCE:direct assay:GO:0055056 D-glucose
transmembrane transporter activity [PMID:12702270]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0005353
fructose transmembrane transporter activity
[PMID:10618490]"
/experiment="EXISTENCE:mutant phenotype:GO:0008645 hexose
transmembrane transport [PMID:10618490]"
/experiment="EXISTENCE:mutant phenotype:GO:0015578 mannose
transmembrane transporter activity [PMID:10618490]"
/experiment="EXISTENCE:mutant phenotype:GO:0055056
D-glucose transmembrane transporter activity
[PMID:20854260|PMID:10618490]"
/experiment="EXISTENCE:mutant phenotype:GO:1904659
D-glucose transmembrane transport [PMID:20854260]"
/note="Hexose transporter with moderate affinity for
glucose; induced in the presence of non-fermentable carbon
sources, induced by a decrease in growth rate, contains an
extended N-terminal domain relative to other HXTs; HXT5
has a paralog, HXT3, that arose from the whole genome
duplication"
/codon_start=1
/product="hexose transporter HXT5"
/protein_id="NP_011964.1"
/db_xref="GeneID:856496"
/db_xref="SGD:S000001138"
/translation="MSELENAHQGPLEGSATVSTNSNSYNEKSGNSTAPGTAGYNDNL
AQAKPVSSYISHEGPPKDELEELQKEVDKQLEKKSKSDLLFVSVCCLMVAFGGFVFGW
DTGTISGFVRQTDFIRRFGSTRANGTTYLSDVRTGLMVSIFNIGCAIGGIVLSKLGDM
YGRKIGLMTVVVIYSIGIIIQIASIDKWYQYFIGRIISGLGVGGITVLAPMLISEVSP
KQLRGTLVSCYQLMITFGIFLGYCTNFGTKNYSNSVQWRVPLGLCFAWSIFMIVGMTF
VPESPRYLVEVGKIEEAKRSLARANKTTEDSPLVTLEMENYQSSIEAERLAGSASWGE
LVTGKPQMFRRTLMGMMIQSLQQLTGDNYFFYYGTTIFQAVGLEDSFETAIVLGVVNF
VSTFFSLYTVDRFGRRNCLLWGCVGMICCYVVYASVGVTRLWPNGQDQPSSKGAGNCM
IVFACFYIFCFATTWAPVAYVLISESYPLRVRGKAMSIASACNWIWGFLISFFTPFIT
SAINFYYGYVFMGCMVFAYFYVFFFVPETKGLTLEEVNEMYEENVLPWKSTKWIPPSR
RTTDYDLDATRNDPRPFYKRMFTKEK"
rep_origin 297060..297179
/note="ARS815; Putative replication origin; identified in
multiple array studies, not yet confirmed by plasmid-based
assay"
/db_xref="SGD:S000130150"
gene complement(<297385..>298609)
/gene="PAL2"
/locus_tag="YHR097C"
/gene_synonym="SCD1"
/db_xref="GeneID:856497"
mRNA complement(join(<297385..298360,298485..>298609))
/gene="PAL2"
/locus_tag="YHR097C"
/gene_synonym="SCD1"
/product="Pal2p"
/transcript_id="NM_001179227.1"
/db_xref="GeneID:856497"
CDS complement(join(297385..298360,298485..298609))
/gene="PAL2"
/locus_tag="YHR097C"
/gene_synonym="SCD1"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0030479 actin
cortical patch [PMID:30679249]"
/experiment="EXISTENCE:direct assay:GO:0034399 nuclear
periphery [PMID:25817432]"
/note="Protein involved in clathrin-mediated endocytosis;
green fluorescent protein (GFP)-fusion protein localizes
to the cytoplasm and the nucleus; YHR097C has a paralog,
PAL1, that arose from the whole genome duplication"
/codon_start=1
/product="Pal2p"
/protein_id="NP_011965.1"
/db_xref="GeneID:856497"
/db_xref="SGD:S000001139"
/translation="MNQTGRTIGGPQNGVNTVINPFRVSPSEDRVSSRDETPRNYNNP
FLNEDDTRRAHNSSVSNSRQERLPSYEEAAGTPKQQAPYPKEKKRSSGSNSHQHNHHH
HRRTSHGHRDKDKQKSKSRTKVKPPKNVDTIDKMDVTGLFGGSFHHDGPFDACTPQRN
KNNKVAPVLAFPADGPNNTVGGRTSKKSTLDEVFGRETVDDDSETLNQLQDRAYLFNK
ANSSTTTLDAIKPNSKNITQFDSKMKTELVHGPITMGLGSTTFLDGAPASSAAIEQDV
INHAQESRRKNSIARKKSLPSRRHLQVNNNNLKLVKTHSGHLEQKDVDDNRTSVPVTA
TQGSGHEDVVKKENTGNKLLRRVKSLKTSKKH"
gene complement(<299145..>301934)
/gene="SFB3"
/locus_tag="YHR098C"
/gene_synonym="LST1"
/db_xref="GeneID:856498"
mRNA complement(<299145..>301934)
/gene="SFB3"
/locus_tag="YHR098C"
/gene_synonym="LST1"
/product="Sfb3p"
/transcript_id="NM_001179228.1"
/db_xref="GeneID:856498"
CDS complement(299145..301934)
/gene="SFB3"
/locus_tag="YHR098C"
/gene_synonym="LST1"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0030127 COPII
vesicle coat [PMID:12426382|PMID:11086000]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0090110
COPII-coated vesicle cargo loading
[PMID:12426382|PMID:12941277]"
/experiment="EXISTENCE:mutant phenotype:GO:0090110
COPII-coated vesicle cargo loading [PMID:12941277]"
/note="Component of the Sec23p-Sfb3p heterodimer of the
COPII vesicle coat; COPII coat is required for cargo
selection during vesicle formation in ER to Golgi
transport; scaffolding function of Lst1p required to
generate vesicles that can accommodate difficult cargo
proteins that include large oligomeric assemblies and
asymmetrically distributed membrane proteins; with Atg40p
acts in autophagy of the ER; homologous to Sec24p and
Sfb2p"
/codon_start=1
/product="Sfb3p"
/protein_id="NP_011966.1"
/db_xref="GeneID:856498"
/db_xref="SGD:S000001140"
/translation="MSQQNILAASVSALSLDESTVHTGGASSKKSRRPHRAYHNFSSG
TVPTLGNSPYTTPQLNQQDGFQQPQAFTPKQFGGFNNGSGSVMSTPVMVSQERFGASE
ASSPYGQSMLDMTAPQPTSHIVPTQRFEDQAQYLQRSFETCRDSVPPLPTTQFYCVDQ
GSCDPHLMSLSMYNIPESEHLRAATKLPLGLTIQPFSTLTPNDAEVPTIPLPMDGTPL
RCRRCRAYANPKFQFTYDSSVICNICRVKMQVPGEHFAPMGPNGQRSDLNEKSELLHG
TVDFLVPSIYNAIQEKELLPLHYVFLIDVSLLANENGSSLAMVEGVRSCIEYISDFQP
NCEVAIIVYDNKLRFFNLRPDLDNAQEYIVSELDDVFLPFYNGLFVKPGNSMKIINDT
LIKISGYISTDKYSHVPQVCYGSALQAAKLALDTVTGGQGGKIICSLNSLPTIGNGNL
SLKRDNAHIAHVKCDNGFYKKLASDFLKSYISLDLYVTNAGFIDMATVGHPVEMTSGI
LKYYPHFQQETDAFTLVNDMVTNVSNIVGYQALLKVRCSTGLSVEQYYCDSSDNTDHD
PIIPVLTRDTTLDVLLKYDSKIKTGTDVHFQTALLYTDIDGVRKVRSINTSGAVSNNI
REIFKFINQNPVMRIMIKDVIKTLGDCDFVKIRRLIDDKMVEILTQYRGLVSSNSSTQ
LILPDSIKTLPAYMLAFEKSELMKPNAQSTRGNERIYDLLKYDSLNSAQLCYKLYPQI
VPFHVLLEETDLTFYDANDKLLQINSSSINNLSVRASHSNFINGGCYLIFQGDTIYLW
FNENTNRMLLQDLLSVDESLPVSQISLFSGTLPETGTSINQKASNVIKNWQQVVNKSS
LPLVLLRPNVDQYYSNVMSQLLCEDKTVNRIESYDNYLVIMHKKIQEKLQKDDFIKVS
TAATHENIHQKFVQF"
gene <302761..>313995
/gene="TRA1"
/locus_tag="YHR099W"
/db_xref="GeneID:856499"
mRNA <302761..>313995
/gene="TRA1"
/locus_tag="YHR099W"
/product="histone acetyltransferase TRA1"
/transcript_id="NM_001179229.1"
/db_xref="GeneID:856499"
CDS 302761..313995
/gene="TRA1"
/locus_tag="YHR099W"
/experiment="EXISTENCE:direct assay:GO:0000124 SAGA
complex [PMID:9885573]"
/experiment="EXISTENCE:direct assay:GO:0004402 histone
acetyltransferase activity [PMID:10911987]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:18616809]"
/experiment="EXISTENCE:direct assay:GO:0006281 DNA repair
[PMID:16135807]"
/experiment="EXISTENCE:direct assay:GO:0035267 NuA4
histone acetyltransferase complex [PMID:10911987]"
/experiment="EXISTENCE:direct assay:GO:0046695 SLIK
(SAGA-like) complex [PMID:12446794]"
/experiment="EXISTENCE:direct assay:GO:0110078 TTT Hsp90
cochaperone complex [PMID:19040720]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:20635087]"
/note="Subunit of SAGA and NuA4 histone acetyltransferase
complexes; subunit of the TTT Hsp90 cochaperone complex
that is involved in chromatin remodeling and telomeric
chromatin regulation; interacts with acidic activators
(e.g., Gal4p) which leads to transcription activation;
similar to human TRRAP, which is a cofactor for c-Myc
mediated oncogenic transformation"
/codon_start=1
/product="histone acetyltransferase TRA1"
/protein_id="NP_011967.1"
/db_xref="GeneID:856499"
/db_xref="SGD:S000001141"
/translation="MSLTEQIEQFASRFRDDDATLQSRYSTLSELYDIMELLNSPEDY
HFFLQAVIPLLLNQLKEVPISYDAHSPEQKLRNSMLDIFNRCLMNQTFQPYAMEVLEF
LLSVLPKENEENGILCMKVLTTLFKSFKSILQDKLDSFIRIIIQIYKNTPNLINQTFY
EAGKAEQGDLDSPKEPQADELLDEFSKNDEEKDFPSKQSSTEPRFENSTSSNGLRSSM
FSFKILSECPITMVTLYSSYKQLTSTSLPEFTPLIMNLLNIQIKQQQEAREQAESRGE
HFTSISTEIINRPAYCDFILAQIKATSFLAYVFIRGYAPEFLQDYVNFVPDLIIRLLQ
DCPSELSSARKELLHATRHILSTNYKKLFLPKLDYLFDERILIGNGFTMHETLRPLAY
STVADFIHNIRSELQLSEIEKTIKIYTGYLLDESLALTVQIMSAKLLLNLVERILKLG
KENPQEAPRAKKLLMIIIDSYMNRFKTLNRQYDTIMKYYGRYETHKKEKAEKLKNSIQ
DNDKESEEFMRKVLEPSDDDHLMPQPKKEDINDSPDVEMTESDKVVKNDVEMFDIKNY
APILLLPTPTNDPIKDAFYLYRTLMSFLKTIIHDLKVFNPPPNEYTVANPKLWASVSR
VFSYEEVIVFKDLFHECIIGLKFFKDHNEKLSPETTKKHFDISMPSLPVSATKDAREL
MDYLAFMFMQMDNATFNEIIEQELPFVYERMLEDSGLLHVAQSFLTSEITSPNFAGIL
LRFLKGKLKDLGNVDFNTSNVLIRLFKLSFMSVNLFPNINEVVLLPHLNDLILNSLKY
STTAEEPLVYFYLIRTLFRSIGGGRFENLYRSIKPILQVLLQSLNQMILTARLPHERE
LYVELCITVPVRLSVLAPYLPFLMKPLVFALQQYPDLVSQGLRTLELCIDNLTAEYFD
PIIEPVIDDVSKALFNLLQPQPFNHAISHNVVRILGKLGGRNRQFLKPPTDLTEKTEL
DIDAIADFKINGMPEDVPLSVTPGIQSALNILQSYKSDIHYRKSAYKYLTCVLLLMTK
SSAEFPTNYTELLKTAVNSIKLERIGIEKNFDLEPTVNKRDYSNQENLFLRLLESVFY
ATSIKELKDDAMDLLNNLLDHFCLLQVNTTLLNKRNYNGTFNIDLKNPNFMLDSSLIL
DAIPFALSYYIPEVREVGVLAYKRIYEKSCLIYGEELALSHSFIPELAKQFIHLCYDE
TYYNKRGGVLGIKVLIDNVKSSSVFLKKYQYNLANGLLFVLKDTQSEAPSAITDSAEK
LLIDLLSITFADVKEEDLGNKVLENTLTDIVCELSNANPKVRNACQKSLHTISNLTGI
PIVKLMDHSKQFLLSPIFAKPLRALPFTMQIGNVDAITFCLSLPNTFLTFNEELFRLL
QESIVLADAEDESLSTNIQKTTEYSTSEQLVQLRIACIKLLAIALKNEEFATAQQGNI
RIRILAVFFKTMLKTSPEIINTTYEALKGSLAENSKLPKELLQNGLKPLLMNLSDHQK
LTVPGLDALSKLLELLIAYFKVEIGRKLLDHLTAWCRVEVLDTLFGQDLAEQMPTKII
VSIINIFHLLPPQADMFLNDLLLKVMLLERKLRLQLDSPFRTPLARYLNRFHNPVTEY
FKKNMTLRQLVLFMCNIVQRPEAKELAEDFEKELDNFYDFYISNIPKNQVRVVSFFTN
MVDLFNTMVITNGDEWLKKKGNMILKLKDMLNLTLKTIKENSFYIDHLQLNQSIAKFQ
ALYLRFTELSERDQNPLLLDFIDFSFSNGIKASYSLKKFIFHNIIASSNKEKQNNFIN
DATLFVLSDKCLDARIFVLKNVINSTLIYEVATSGSLKSYLVEDKKPKWLELLHNKIW
KNSNAILAYDVLDHHDLFRFELLQLSAIFIKADPEIIAEIKKDIIKFCWNFIKLEDTL
IKQSAYLVTSYFISKFDFPIKVVTQVFVALLRSSHVEARYLVKQSLDVLTPVLHERMN
AAGTPDTWINWVKRVMVENSSSQNNILYQFLISHPDLFFNSRDLFISNIIHHMNKITF
MSNSNSDSHTLAIDLASLILYWENKTLEITNVNNTKTDSDGDVVMSDSKSDINPVEAD
TTAIIVDANNNSPISLHLREACTAFLIRYVCASNHRAIETELGLRAINILSELISDKH
WTNVNVKLVYFEKFLIFQDLDSENILYYCMNALDVLYVFFKNKTKEWIMENLPTIQNL
LEKCIKSDHHDVQEALQKVLQVIMKAIKAQGVSVIIEEESPGKTFIQMLTSVITQDLQ
ETSSVTAGVTLAWVLFMNFPDNIVPLLTPLMKTFSKLCKDHLSISQPKDAMALEEARI
TTKLLEKVLYILSLKVSLLGDSRRPFLSTVALLIDHSMDQNFLRKIVNMSRSWIFNTE
IFPTVKEKAAILTKMLAFEIRGEPSLSKLFYEIVLKLFDQEHFNNTEITVRMEQPFLV
GTRVEDIGIRKRFMTILDNSLERDIKERLYYVIRDQNWEFIADYPWLNQALQLLYGSF
NREKELSLKNIYCLSPPSILQEYLPENAEMVTEVNDLELSNFVKGHIASMQGLCRIIS
SDFIDSLIEIFYQDPKAIHRAWVTLFPQVYKSIPKNEKYGFVRSIITLLSKPYHTRQI
SSRTNVINMLLDSISKIESLELPPHLVKYLAISYNAWYQSINILESIQSNTSIDNTKI
IEANEDALLELYVNLQEEDMFYGLWRRRAKYTETNIGLSYEQIGLWDKAQQLYEVAQV
KARSGALPYSQSEYALWEDNWIQCAEKLQHWDVLTELAKHEGFTDLLLECGWRVADWN
SDRDALEQSVKSVMDVPTPRRQMFKTFLALQNFAESRKGDQEVRKLCDEGIQLSLIKW
VSLPIRYTPAHKWLLHGFQQYMEFLEATQIYANLHTTTVQNLDSKAQEIKRILQAWRD
RLPNTWDDVNMWNDLVTWRQHAFQVINNAYLPLIPALQQSNSNSNINTHAYRGYHEIA
WVINRFAHVARKHNMPDVCISQLARIYTLPNIEIQEAFLKLREQAKCHYQNMNELTTG
LDVISNTNLVYFGTVQKAEFFTLKGMFLSKLRAYEEANQAFATAVQIDLNLAKAWAQW
GFFNDRRLSEEPNNISFASNAISCYLQAAGLYKNSKIRELLCRILWLISIDDASGMLT
NAFDSFRGEIPVWYWITFIPQLLTSLSHKEANMVRHILIRIAKSYPQALHFQLRTTKE
DFAVIQRQTMAVMGDKPDTNDRNGRRQPWEYLQELNNILKTAYPLLALSLESLVAQIN
DRFKSTTDEDLFRLINVLLIDGTLNYNRLPFPRKNPKLPENTEKNLVKFSTTLLAPYI
RPKFNADFIDNKPDYETYIKRLRYWRRRLENKLDRASKKENLEVLCPHLSNFHHQKFE
DIEIPGQYLLNKDNNVHFIKIARFLPTVDFVRGTHSSYRRLMIRGHDGSVHSFAVQYP
AVRHSRREERMFQLYRLFNKSLSKNVETRRRSIQFNLPIAIPLSPQVRIMNDSVSFTT
LHEIHNEFCKKKGFDPDDIQDFMADKLNAAHDDALPAPDMTILKVEIFNSIQTMFVPS
NVLKDHFTSLFTQFEDFWLFRKQFASQYSSFVFMSYMMMINNRTPHKIHVDKTSGNVF
TLEMLPSRFPYERVKPLLKNHDLSLPPDSPIFHNNEPVPFRLTPNIQSLIGDSALEGI
FAVNLFTISRALIEPDNELNTYLALFIRDEIISWFSNLHRPIIENPQLREMVQTNVDL
IIRKVAQLGHLNSTPTVTTQFILDCIGSAVSPRNLARTDVNFMPWF"
gene complement(<314116..>314673)
/gene="GEP4"
/locus_tag="YHR100C"
/db_xref="GeneID:856500"
mRNA complement(<314116..>314673)
/gene="GEP4"
/locus_tag="YHR100C"
/product="phosphatidylglycerophosphatase"
/transcript_id="NM_001179230.1"
/db_xref="GeneID:856500"
CDS complement(314116..314673)
/gene="GEP4"
/locus_tag="YHR100C"
/EC_number="3.1.3.27"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:20485265]"
/experiment="EXISTENCE:direct assay:GO:0008962
phosphatidylglycerophosphatase activity [PMID:20485265]"
/experiment="EXISTENCE:genetic interaction:GO:0008962
phosphatidylglycerophosphatase activity [PMID:20485265]"
/experiment="EXISTENCE:mutant phenotype:GO:0008962
phosphatidylglycerophosphatase activity [PMID:20485265]"
/experiment="EXISTENCE:mutant phenotype:GO:0032049
cardiolipin biosynthetic process [PMID:20485265]"
/note="Mitochondrial phosphatidylglycerophosphatase (PGP
phosphatase); dephosphorylates
phosphatidylglycerolphosphate to generate
phosphatidylglycerol, an essential step during cardiolipin
biosynthesis; null mutant is sensitive to tunicamycin,
DTT"
/codon_start=1
/product="phosphatidylglycerophosphatase"
/protein_id="NP_011968.1"
/db_xref="GeneID:856500"
/db_xref="SGD:S000001142"
/translation="MNISGTLNTLRLLYNPSLCKPSLVVPTFNDLPIPIHDSIKAVVL
DKDNCIAFPHDDKIWPDYLQHWETLRSKYSNKALLIVSNTAGSNSDKDYSQAKLLEDK
TGIPVLRHSTKKPGCHNEILDYFYRNKTITNPKEVAVVGDRLFTDILMANLMGSYGVW
IRDGVKVSANPLSKFEKKLYNFLGF"
gene complement(<314874..>315968)
/gene="BIG1"
/locus_tag="YHR101C"
/db_xref="GeneID:856501"
mRNA complement(join(<314874..315771,315859..>315968))
/gene="BIG1"
/locus_tag="YHR101C"
/product="Big1p"
/transcript_id="NM_001179231.1"
/db_xref="GeneID:856501"
CDS complement(join(314874..315771,315859..315968))
/gene="BIG1"
/locus_tag="YHR101C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:12112232]"
/experiment="EXISTENCE:genetic interaction:GO:0006078
(1->6)-beta-D-glucan biosynthetic process [PMID:12112232]"
/experiment="EXISTENCE:genetic interaction:GO:0070072
vacuolar proton-transporting V-type ATPase complex
assembly [PMID:39259764]"
/experiment="EXISTENCE:mutant phenotype:GO:0006078
(1->6)-beta-D-glucan biosynthetic process [PMID:12112232]"
/experiment="EXISTENCE:mutant phenotype:GO:0070072
vacuolar proton-transporting V-type ATPase complex
assembly [PMID:39259764]"
/experiment="EXISTENCE:physical interaction:GO:0070072
vacuolar proton-transporting V-type ATPase complex
assembly [PMID:39259764]"
/note="Integral ER membrane protein; involved in the
assembly and function of the V-ATPase; proposed role as a
signaling component that regulates autophagy independent
of TOR; required for biosynthesis of cell wall
beta-1,6-glucan; functional homolog of human ATP6AP1;
analogous follicular lymphoma-associated mutation induces
autophagy"
/codon_start=1
/product="Big1p"
/protein_id="NP_011969.1"
/db_xref="GeneID:856501"
/db_xref="SGD:S000001143"
/translation="MQTVLKYLLLIMCGSFCASEELQNQTNVPAIFFSYKLTPGILKY
QEDYDRAVTLPRDTFIEAAEKFLGVCNADTYVFINQPGLRKLDFLEFETEFVSLQRYI
RRSSTAIKFEKVDLLPQDLYYDLAEFVKEYCNVDQVLNLRGNNTEDFQPFIDSEKRVI
IIEYPKLPEDTNERKEAFRHYDKYLRTILAQIPSPEQNVIYTSLNPGTTLAHESIIPI
QIFPDIFDIKSRVGEVEQNNRVLDVPRLSFNDYTPRFSEPPSEYVSIFDSQLIENNRG
LLQLIFTILVGYILIQFFFTKKTIVDEKITNKKDNVKQTSPQLLKKVQEIQKKPSQQV
S"
gene <316572..>319814
/gene="KIC1"
/locus_tag="YHR102W"
/gene_synonym="NRK1"
/db_xref="GeneID:856502"
mRNA <316572..>319814
/gene="KIC1"
/locus_tag="YHR102W"
/gene_synonym="NRK1"
/product="putative serine/threonine protein kinase KIC1"
/transcript_id="NM_001179232.1"
/db_xref="GeneID:856502"
CDS 316572..319814
/gene="KIC1"
/locus_tag="YHR102W"
/gene_synonym="NRK1"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:12972564]"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:9813095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005933 cellular
bud [PMID:12972564]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:12972564]"
/experiment="EXISTENCE:genetic interaction:GO:0007118
budding cell apical bud growth [PMID:12972564]"
/experiment="EXISTENCE:mutant phenotype:GO:0007118 budding
cell apical bud growth [PMID:12972564]"
/experiment="EXISTENCE:mutant phenotype:GO:0031505
fungal-type cell wall organization [PMID:12480907]"
/note="Protein kinase of the PAK/Ste20 family, required
for cell integrity; physically interacts with Cdc31p
(centrin), which is a component of the spindle pole body;
phosphorylates Cbk1p kinase after allosteric activation by
Hym1p; part of the RAM network that regulates cellular
polarity and morphogenesis"
/codon_start=1
/product="putative serine/threonine protein kinase KIC1"
/protein_id="NP_011970.1"
/db_xref="GeneID:856502"
/db_xref="SGD:S000001144"
/translation="MTTKPQNSKQGLAEGEMDVSSLFKRTEVIGRGKFGVVYKGYNVK
TGRVYAIKVLNLDSDSDEVEDVQREIQFLASLKQISNITRYYGSYLKDTSLWIIMEHC
AGGSLRSLLRPGKIDEKYIGVIMRELLVALKCIHKDNVIHRDIKAANVLITNEGNVKL
CDFGVAAQVNQTSLRRQTMAGTPYWMAPEVIMEGVYYDTKVDIWSLGITTYEIATGNP
PYCDVEALRAMQLIIKSKPPRLEDRSYSTSLKEFIALCLDEDPKERLSADDLLKSKFI
RAHKATPTSILKELISRYLLFRDKNKNKYKIEGSIPENEPSKPSEAPKPSQNGGGDEA
QKSIASNDNEIKRVNEGDVEMKWDFDSLSSSDYIIENNINLDALAEDNNEWATAQHDL
FNYAYPDEDSYYFDPTSHNTRPFVYQGTTIGKGYPGTIAQNSTLNAPVTNNYTNSKYP
SKMVAGTTNTSGTHTAGPMTSSKRLESKAPKQLLELFEDNEIITAENDVNTEAPKISK
SISSLNAGNSSRDDFIPSISNEVNGNINNNKMRPHLPPLSSGNNYYSQSTPALPLLQT
KFNKTSKGPPTSGLTTAPTSIEIEIPEELPNSALPTPASADPVLIPSTKARSSTVTAG
TPSSSSSIQYKSPSNVPRRLTVSNNRPEHCPSTITNQKLGSAVASNSGISSTPNNSNN
YNNNTDSENSRGSSGSNTANSTQMGITNPGNVTKLSTHKASSPSRPLFGVGTSPNRKP
AGSPTQNIGHNSTHTNLAPPPTMKPMANSKDNKDILLQPLNSIPSSSTLNTISGNSSN
NLTSSNYFSNEKEGSRVNGDFKRNNPNLKLQMPLPTPVVRNKLLDPNTATSQNNNGMP
GSAGISTNENINQFGFNTSSASNIPVSMTPISEKHIDFGGKIKRSQSISNRKNSSASE
HPLNILGSSVSGNVSGIGNNNVGSNNNSGPNNSVPLSANTGNTTIKANSTTIATSSSA
AASTTAPISQQTIPSGTQFNHILSSAATAANSVNSLGFGMCPPPQSLQMEMFLDLESF
LPGKQRRVDRKPQVLKELENLLQMFEEGLPCIEHALKEQLISTPIKDNEH"
gene <320414..>322972
/gene="SBE22"
/locus_tag="YHR103W"
/db_xref="GeneID:856503"
mRNA <320414..>322972
/gene="SBE22"
/locus_tag="YHR103W"
/product="Sbe22p"
/transcript_id="NM_001179233.1"
/db_xref="GeneID:856503"
CDS 320414..322972
/gene="SBE22"
/locus_tag="YHR103W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0031505
fungal-type cell wall organization [PMID:10679005]"
/note="Protein involved in bud growth; involved in the
transport of cell wall components from the Golgi to the
cell surface; similar in structure and functionally
redundant with Sbe2p; SBE22 has a paralog, SBE2, that
arose from the whole genome duplication"
/codon_start=1
/product="Sbe22p"
/protein_id="NP_011971.1"
/db_xref="GeneID:856503"
/db_xref="SGD:S000001145"
/translation="MTSIQERGTSAHLHSLKEGEASDRSSEMLPKQRSIIGSHVQRPP
SQTTLGRSRAGSNTMNKVSGLDIARRPSENLLSNMNCSDNGNGGNMLNSFVNSALPPP
KVNPAQTRRERPASNSSIGTKTTEVFSSTSASSSLGDTSDEGEGSDADKSKINTFPSI
LMEKATQGRGADGNGMRSASNNTIVEATTDGSKMALQKSMSFDDTAAEKTMNKSRHSY
QEQFSSKKSQSSLLNSKQRSRAKSQTCSSTGYNNSSILKTFGISSKISNSSDRIEASS
LEFNVPSQKPLNCKPLTPSQKYRLRKEQSEMNLRNTIKRKEKFYDSQEQILELQEGDV
DDSLIWNVPMASLSTNSFLASAKPDDMNNLAGKNDLSEYTGGLVNDNSEISYTKQNHR
YSNISFASTTSNASLLDFNEMPTSPIPGLNKVTDFQFIQDTTKSLASVYLHSSNRLSR
SKLSERTKSSDFLPIELKEAQNQGMEDLILVSENKLDVVSHSRPSWLPPKDRQEKKLH
ERQINKSMSVASLDQLGKNKDREEKLIRDETNRQKYVLLLDRDITRNSSLQSLSKMVW
DTPFSDETRSTIYSEILQSKTRFITKNYIQPFHELQELLTKMGDFPKNKEIEISQLIE
TSLRRKVSGLHDICPDLMLLLKIKSISSQGIVTGDELLFHHFLVSESFQNLGLNEIWN
IVNLVQMTCFNDLCKEKFDAKVLERKGVVAGYLSQNEEFKDEFNTECINSTTWWNILE
RIDHKLFMWIMDIIVVNNSQSYKNSPINEDEFVNKDWEYYRSKKVVINYKILISFALN
VLLNYHFGFTDLRSLCNVNDQRFCIPVFINDEFVDADTVNAVFIKKWAHYYKKF"
gene <323409..>324392
/gene="GRE3"
/locus_tag="YHR104W"
/db_xref="GeneID:856504"
mRNA <323409..>324392
/gene="GRE3"
/locus_tag="YHR104W"
/product="trifunctional aldehyde reductase/xylose
reductase/glucose 1-dehydrogenase (NADP(+))"
/transcript_id="NM_001179234.1"
/db_xref="GeneID:856504"
CDS 323409..324392
/gene="GRE3"
/locus_tag="YHR104W"
/EC_number="1.1.1.21"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:21124907]"
/experiment="EXISTENCE:direct assay:GO:0004032 aldose
reductase (NADPH) activity [PMID:11306085|PMID:11525399]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0019568 arabinose
catabolic process [PMID:12271459]"
/experiment="EXISTENCE:direct assay:GO:0032866 D-xylose
reductase (NADPH) activity [PMID:11481678]"
/experiment="EXISTENCE:direct assay:GO:0042843 D-xylose
catabolic process [PMID:12271459]"
/experiment="EXISTENCE:direct assay:GO:0071470 cellular
response to osmotic stress [PMID:11525399]"
/experiment="EXISTENCE:genetic interaction:GO:0019388
galactose catabolic process [PMID:18811659]"
/experiment="EXISTENCE:genetic interaction:GO:0034599
cellular response to oxidative stress [PMID:17919749]"
/experiment="EXISTENCE:mutant phenotype:GO:0019568
arabinose catabolic process [PMID:12271459]"
/experiment="EXISTENCE:mutant phenotype:GO:0032866
D-xylose reductase (NADPH) activity [PMID:11481678]"
/experiment="EXISTENCE:mutant phenotype:GO:0042843
D-xylose catabolic process [PMID:12271459]"
/note="Aldose reductase; involved in methylglyoxal,
d-xylose, arabinose, and galactose metabolism; stress
induced (osmotic, ionic, oxidative, heat shock, starvation
and heavy metals); regulated by HOG pathway;
overexpression allows xylose fermentation in strains
expressing heterologous xylitol dehydrogenase and
xylulokinase; protein abundance increases in response to
DNA replication stress"
/codon_start=1
/product="trifunctional aldehyde reductase/xylose
reductase/glucose 1-dehydrogenase (NADP(+))"
/protein_id="NP_011972.1"
/db_xref="GeneID:856504"
/db_xref="SGD:S000001146"
/translation="MSSLVTLNNGLKMPLVGLGCWKIDKKVCANQIYEAIKLGYRLFD
GACDYGNEKEVGEGIRKAISEGLVSRKDIFVVSKLWNNFHHPDHVKLALKKTLSDMGL
DYLDLYYIHFPIAFKYVPFEEKYPPGFYTGADDEKKGHITEAHVPIIDTYRALEECVD
EGLIKSIGVSNFQGSLIQDLLRGCRIKPVALQIEHHPYLTQEHLVEFCKLHDIQVVAY
SSFGPQSFIEMDLQLAKTTPTLFENDVIKKVSQNHPGSTTSQVLLRWATQRGIAVIPK
SSKKERLLGNLEIEKKFTLTEQELKDISALNANIRFNDPWTWLDGKFPTFA"
gene <324766..>325410
/gene="YPT35"
/locus_tag="YHR105W"
/db_xref="GeneID:856505"
mRNA <324766..>325410
/gene="YPT35"
/locus_tag="YHR105W"
/product="Ypt35p"
/transcript_id="NM_001179235.1"
/db_xref="GeneID:856505"
CDS 324766..325410
/gene="YPT35"
/locus_tag="YHR105W"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0032266
phosphatidylinositol-3-phosphate binding [PMID:11557775]"
/note="Endosomal hypothetical protein; contains a phox
(PX) homology domain; binds to both
phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins
involved in ER-Golgi or vesicular transport"
/codon_start=1
/product="Ypt35p"
/protein_id="NP_011973.1"
/db_xref="GeneID:856505"
/db_xref="SGD:S000001147"
/translation="MSDKISFLPPEPIQLLDEDSTEPELDIDSQQENEGPISASNSND
STSHSNDCGATITRTRPRRSSSINANFSFQKAHVSDCTIVNGDHGTKFAVWRITVFLE
PNLKAFAAKRESYKIQTYKRYSDFVRLRENLLTRIKTAKPEKLNCLQIPHLPPSVQWY
SSWKYQEVNLNKDWLAKRQRGLEYFLNHIILNSSLVEMTKDILIQFLEPSKRVA"
gene <325598..>326626
/gene="TRR2"
/locus_tag="YHR106W"
/db_xref="GeneID:856506"
mRNA <325598..>326626
/gene="TRR2"
/locus_tag="YHR106W"
/product="thioredoxin-disulfide reductase TRR2"
/transcript_id="NM_001179236.1"
/db_xref="GeneID:856506"
CDS 325598..326626
/gene="TRR2"
/locus_tag="YHR106W"
/EC_number="1.8.1.9"
/experiment="EXISTENCE:direct assay:GO:0004791
thioredoxin-disulfide reductase (NADPH) activity
[PMID:10037727]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239|PMID:10037727]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress [PMID:10037727]"
/note="Mitochondrial thioredoxin reductase; involved in
protection against oxidative stress, required with Glr1p
to maintain the redox state of Trx3p; contains active-site
motif (CAVC) present in prokaryotic orthologs; binds NADPH
and FAD; TRR2 has a paralog, TRR1, that arose from the
whole genome duplication"
/codon_start=1
/product="thioredoxin-disulfide reductase TRR2"
/protein_id="NP_011974.1"
/db_xref="GeneID:856506"
/db_xref="SGD:S000001148"
/translation="MIKHIVSPFRTNFVGISKSVLSRMIHHKVTIIGSGPAAHTAAIY
LARAEMKPTLYEGMMANGIAAGGQLTTTTDIENFPGFPESLSGSELMERMRKQSAKFG
TNIITETVSKVDLSSKPFRLWTEFNEDAEPVTTDAIILATGASAKRMHLPGEETYWQQ
GISACAVCDGAVPIFRNKPLAVIGGGDSACEEAEFLTKYASKVYILVRKDHFRASVIM
QRRIEKNPNIIVLFNTVALEAKGDGKLLNMLRIKNTKSNVENDLEVNGLFYAIGHSPA
TDIVKGQVDEEETGYIKTVPGSSLTSVPGFFAAGDVQDSRYRQAVTSAGSGCIAALDA
ERYLSAQE"
gene complement(<326813..>328036)
/gene="CDC12"
/locus_tag="YHR107C"
/gene_synonym="CLA10; PSL7"
/db_xref="GeneID:856507"
mRNA complement(<326813..>328036)
/gene="CDC12"
/locus_tag="YHR107C"
/gene_synonym="CLA10; PSL7"
/product="septin CDC12"
/transcript_id="NM_001179237.1"
/db_xref="GeneID:856507"
CDS complement(326813..328036)
/gene="CDC12"
/locus_tag="YHR107C"
/gene_synonym="CLA10; PSL7"
/experiment="EXISTENCE:direct assay:GO:0000144 cellular
bud neck septin ring [PMID:3316985]"
/experiment="EXISTENCE:direct assay:GO:0000921 septin ring
assembly [PMID:12636916]"
/experiment="EXISTENCE:direct assay:GO:0001934 positive
regulation of protein phosphorylation [PMID:14527412]"
/experiment="EXISTENCE:direct assay:GO:0003924 GTPase
activity [PMID:14993234]"
/experiment="EXISTENCE:direct assay:GO:0005200 structural
constituent of cytoskeleton
[PMID:18550837|PMID:29321251|PMID:22144691]"
/experiment="EXISTENCE:direct assay:GO:0005525 GTP binding
[PMID:14597621]"
/experiment="EXISTENCE:direct assay:GO:0005621 cellular
bud scar [PMID:23345395]"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005937 mating
projection [PMID:18552279]"
/experiment="EXISTENCE:direct assay:GO:0010314
phosphatidylinositol-5-phosphate binding [PMID:12665577]"
/experiment="EXISTENCE:direct assay:GO:0031105 septin
complex [PMID:18550837]"
/experiment="EXISTENCE:direct assay:GO:0032160 septin
filament array [PMID:15282341]"
/experiment="EXISTENCE:direct assay:GO:0070273
phosphatidylinositol-4-phosphate binding [PMID:12665577]"
/experiment="EXISTENCE:mutant phenotype:GO:0000281 mitotic
cytokinesis [PMID:4950437]"
/experiment="EXISTENCE:mutant phenotype:GO:0000921 septin
ring assembly [PMID:14993234]"
/experiment="EXISTENCE:mutant phenotype:GO:0030011
maintenance of cell polarity [PMID:10882120]"
/experiment="EXISTENCE:mutant phenotype:GO:0060090
molecular adaptor activity [PMID:9442111]"
/experiment="EXISTENCE:mutant phenotype:GO:1903475 mitotic
actomyosin contractile ring assembly [PMID:9864366]"
/experiment="EXISTENCE:physical interaction:GO:0031105
septin complex [PMID:9813094]"
/note="Component of the septin ring that is required for
cytokinesis; septins are GTP-binding proteins that
assemble into rod-like hetero-oligomers that can associate
with other rods to form filaments; septin rings at the
mother-bud neck act as scaffolds for recruiting cell
division factors and as barriers to prevent diffusion of
specific proteins between mother and daughter cells"
/codon_start=1
/product="septin CDC12"
/protein_id="NP_011975.1"
/db_xref="GeneID:856507"
/db_xref="SGD:S000001149"
/translation="MSAATATAAPVPPPVGISNLPNQRYKIVNEEGGTFTVMLCGESG
LGKTTFINTLFQTVLKRADGQQHRQEPIRKTVEIDITRALLEEKHFELRVNVIDTPGF
GDNVNNNKAWQPLVDFIDDQHDSYMRQEQQPYRTKKFDLRVHAVLYFIRPTGHGLKPI
DIETMKRLSTRANLIPVIAKADTLTAQELQQFKSRIRQVIEAQEIRIFTPPLDADSKE
DAKSGSNPDSAAVEHARQLIEAMPFAIVGSEKKFDNGQGTQVVARKYPWGLVEIENDS
HCDFRKLRALLLRTYLLDLISTTQEMHYETYRRLRLEGHENTGEGNEDFTLPAIAPAR
KLSHNPRYKEEENALKKYFTDQVKAEEQRFRQWEQNIVNERIRLNGDLEEIQGKVKKL
EEQVKSLQVKKSHLK"
gene <328303..>330060
/gene="GGA2"
/locus_tag="YHR108W"
/db_xref="GeneID:856508"
mRNA <328303..>330060
/gene="GGA2"
/locus_tag="YHR108W"
/product="phosphatidylinositol 4-phosphate-binding
protein"
/transcript_id="NM_001179238.1"
/db_xref="GeneID:856508"
CDS 328303..330060
/gene="GGA2"
/locus_tag="YHR108W"
/experiment="EXISTENCE:direct assay:GO:0005802 trans-Golgi
network [PMID:10747088]"
/experiment="EXISTENCE:direct assay:GO:0043130 ubiquitin
binding [PMID:15039776]"
/experiment="EXISTENCE:direct assay:GO:0070273
phosphatidylinositol-4-phosphate binding [PMID:18287542]"
/experiment="EXISTENCE:genetic interaction:GO:0006896
Golgi to vacuole transport [PMID:10747088]"
/experiment="EXISTENCE:mutant phenotype:GO:0006895 Golgi
to endosome transport [PMID:15039776]"
/experiment="EXISTENCE:mutant phenotype:GO:0006896 Golgi
to vacuole transport [PMID:10747088]"
/experiment="EXISTENCE:mutant phenotype:GO:0043130
ubiquitin binding [PMID:19574226]"
/experiment="EXISTENCE:mutant phenotype:GO:0043328 protein
transport to vacuole involved in ubiquitin-dependent
protein catabolic process via the multivesicular body
sorting pathway [PMID:15039776]"
/experiment="EXISTENCE:mutant phenotype:GO:0070273
phosphatidylinositol-4-phosphate binding [PMID:18287542]"
/note="Protein that regulates Arf1p and Arf2p to
facilitate Golgi trafficking; binds phosphatidylinositol
4-phosphate, which plays a role in TGN localization;
K63-specific Ub chain binding receptor with moderate
selectivity; homologous to gamma-adaptin; GGA2 has a
paralog, GGA1, that arose from the whole genome
duplication"
/codon_start=1
/product="phosphatidylinositol 4-phosphate-binding
protein"
/protein_id="NP_011976.1"
/db_xref="GeneID:856508"
/db_xref="SGD:S000001150"
/translation="MSHPHSHSIYLSELPVRKPQALGNPLLRKIQRACRMSLAEPDLA
LNLDIADYINEKQGAAPRDAAIALAKLINNRESHVAIFALSLLDVLVKNCGYPFHLQI
SRKEFLNELVKRFPGHPPLRYSKIQRLILTAIEEWYQTICKHSSYKNDMGYIRDMHRL
LKYKGYAFPKISESDLAVLKPSNQLKTASEIQKEQEIAQAAKLEELIRRGKPEDLREA
NKLMKIMAGFKEDNAVQAKQAISSELNKLKRKADLLNEMLESPDSQNWDNETTQELHS
ALKVAQPKFQKIIEEEQEDDALVQDLLKFNDTVNQLLEKFNLLKNGDSNAASQIHPSH
VSAPLQQSSGALTNEINLIDFNDLDEAPSQGNNNTNGTGTPAAAETSVNDLLGDLTDL
SISNPSTANQASFGLGGDIVLGSSQPAPPVTTTNNSNNTLDLLGLSTPQSPTNSQAVN
SSGFDLLMGFNPTTGTTTAPARTLVNQSPNLKIEFEISRESNSVIRIKSFFTNLSSSP
ISNLVFLLAVPKSMSLKLQPQSSNFMIGNAKDGISQEGTIENAPANPSKALKVKWKVN
YSVNSTQAEETAVFTLPNV"
gene <330310..>332067
/gene="CTM1"
/locus_tag="YHR109W"
/db_xref="GeneID:856509"
mRNA <330310..>332067
/gene="CTM1"
/locus_tag="YHR109W"
/product="cytochrome c lysine N-methyltransferase"
/transcript_id="NM_001179239.1"
/db_xref="GeneID:856509"
CDS 330310..332067
/gene="CTM1"
/locus_tag="YHR109W"
/EC_number="2.1.1.59"
/experiment="EXISTENCE:direct assay:GO:0000277 [cytochrome
c]-lysine N-methyltransferase activity [PMID:10791961]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:10791961]"
/experiment="EXISTENCE:direct assay:GO:0018022
peptidyl-lysine methylation [PMID:10791961]"
/experiment="EXISTENCE:mutant phenotype:GO:0000277
[cytochrome c]-lysine N-methyltransferase activity
[PMID:10791961]"
/note="Cytochrome c lysine methyltransferase;
trimethylates residue 72 of apo-cytochrome c (Cyc1p) in
the cytosol; not required for normal respiratory growth"
/codon_start=1
/product="cytochrome c lysine N-methyltransferase"
/protein_id="NP_011977.1"
/db_xref="GeneID:856509"
/db_xref="SGD:S000001151"
/translation="MEEVFRFYSNSRNIFIHKSLSLKPSTIDDPKSGYGLFVEPSKFK
NDELKSETIQLLRIPKRCTFNINTLLALLGDEDEFSSKEEFQRTNDKIKIALREIMAH
PNFSVFLTETNLLIIYFMIFQTIRSRYEIPENIQYYLENVLMSIEVETAMDSIENLAT
DYGHYPQIFGLRETLNLFKELFHDVLNLSDIKHLYSAIISRCLEIPERADTKSEEFTV
HSTLVPIVDFANHEGTQKNAYFDIDPSNNDVLLLLDTKAVQSELTKPIEVFISYSPTE
DLFSMLVTYGFTPDFRGNSQFWTVSFDRCFLRNYDGPDKTTNLRLFYKWMHINPVVPL
VKYEHNGKTRWFLNDTTPEFDMLLLPFIPSIDDGKIARWAYDSTCHLMFTKIHCLINP
EANEHALMIAENYRSLIKEKESNGDDFINLPPLAWSLRYKDTENDCVRQRHICSEDAV
AVLKQEEMQDSTKTKSQFTSFFRKFLEFRRSKIIRPTSDSKVASILYQQELEIIADLA
KAIDSSSTIFFSDLNVTLDTEPERLPPLRFLDDYIEISADKQEPSPICEDLSYYTPSR
FTDFFQEEVSQYAAFFQDD"
gene <332282..>332920
/gene="ERP5"
/locus_tag="YHR110W"
/db_xref="GeneID:856510"
mRNA <332282..>332920
/gene="ERP5"
/locus_tag="YHR110W"
/product="Erp5p"
/transcript_id="NM_001179240.1"
/db_xref="GeneID:856510"
CDS 332282..332920
/gene="ERP5"
/locus_tag="YHR110W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/note="Protein with similarity to Emp24p and Erv25p;
member of the p24 family involved in ER to Golgi
transport"
/codon_start=1
/product="Erp5p"
/protein_id="NP_011978.1"
/db_xref="GeneID:856510"
/db_xref="SGD:S000001152"
/translation="MKYNIVHGICLLFAITQAVGAVHFYAKSGETKCFYEHLSRGNLL
IGDLDLYVEKDGLFEEDPESSLTITVDETFDNDHRVLNQKNSHTGDVTFTALDTGEHR
FCFTPFYSKKSATLRVFIELEIGNVEALDSKKKEDMNSLKGRVGQLTQRLSSIRKEQD
AIREKEAEFRNQSESANSKIMTWSVFQLLILLGTCAFQLRYLKNFFVKQKVV"
gene <333072..>334394
/gene="UBA4"
/locus_tag="YHR111W"
/gene_synonym="YHR1"
/db_xref="GeneID:856511"
mRNA <333072..>334394
/gene="UBA4"
/locus_tag="YHR111W"
/gene_synonym="YHR1"
/product="Uba4p"
/transcript_id="NM_001179241.1"
/db_xref="GeneID:856511"
CDS 333072..334394
/gene="UBA4"
/locus_tag="YHR111W"
/gene_synonym="YHR1"
/experiment="EXISTENCE:direct assay:GO:0004792
thiosulfate-cyanide sulfurtransferase activity
[PMID:19864628]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0032447 protein
urmylation [PMID:10713047]"
/experiment="EXISTENCE:direct assay:GO:0042292 URM1
activating enzyme activity [PMID:10713047]"
/experiment="EXISTENCE:genetic interaction:GO:0001403
invasive growth in response to glucose limitation
[PMID:14551258]"
/experiment="EXISTENCE:genetic interaction:GO:0007114 cell
budding [PMID:14551258]"
/experiment="EXISTENCE:mutant phenotype:GO:0001403
invasive growth in response to glucose limitation
[PMID:14551258]"
/experiment="EXISTENCE:mutant phenotype:GO:0002143 tRNA
wobble position uridine thiolation
[PMID:19145231|PMID:19151091|PMID:18664566]"
/experiment="EXISTENCE:mutant phenotype:GO:0016783
sulfurtransferase activity [PMID:19145231]"
/experiment="EXISTENCE:mutant phenotype:GO:0032447 protein
urmylation [PMID:14555475]"
/experiment="EXISTENCE:mutant phenotype:GO:0034227 tRNA
thio-modification [PMID:19145231]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress [PMID:14555475]"
/experiment="EXISTENCE:mutant phenotype:GO:2000220
regulation of pseudohyphal growth [PMID:22043304]"
/note="E1-like protein that activates Urm1p before
urmylation; also acts in thiolation of the wobble base of
cytoplasmic tRNAs by adenylating and then thiolating
Urm1p; receives sulfur from Tum1p"
/codon_start=1
/product="Uba4p"
/protein_id="NP_011979.1"
/db_xref="GeneID:856511"
/db_xref="SGD:S000001153"
/translation="MNDYHLEDTTSELEALRLENAQLREQLAKREDSSRDYPLSLEEY
QRYGRQMIVEETGGVAGQVKLKNTKVLVVGAGGLGCPALPYLAGAGVGQIGIVDNDVV
ETSNLHRQVLHDSSRVGMLKCESARQYITKLNPHINVVTYPVRLNSSNAFDIFKGYNY
ILDCTDSPLTRYLVSDVAVNLGITVVSASGLGTEGQLTILNFNNIGPCYRCFYPTPPP
PNAVTSCQEGGVIGPCIGLVGTMMAVETLKLILGIYTNENFSPFLMLYSGFPQQSLRT
FKMRGRQEKCLCCGKNRTITKEAIEKGEINYELFCGARNYNVCEPDERISVDAFQRIY
KDDEFLAKHIFLDVRPSHHYEISHFPEAVNIPIKNLRDMNGDLKKLQEKLPSVEKDSN
IVILCRYGNDSQLATRLLKDKFGFSNVRDVRGGYFKYIDDIDQTIPKY"
gene complement(<334527..>335663)
/locus_tag="YHR112C"
/db_xref="GeneID:856512"
mRNA complement(<334527..>335663)
/locus_tag="YHR112C"
/product="putative cystathionine beta-lyase"
/transcript_id="NM_001179242.1"
/db_xref="GeneID:856512"
CDS complement(334527..335663)
/locus_tag="YHR112C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:21777356]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:21777356]"
/note="hypothetical protein; localizes to the cytoplasm
and nucleus; overexpression affects protein trafficking
through the endocytic pathway"
/codon_start=1
/product="putative cystathionine beta-lyase"
/protein_id="NP_011980.1"
/db_xref="GeneID:856512"
/db_xref="SGD:S000001154"
/translation="MVDLSTALIHGDDKDNRVTDVAPPINVSTTFRYDDDDLIPWTER
ENLDFMEKKPVYSRLAHPNSTRLESIFSEILDGYAVIYSSGLAAFYAAMVHYNPKKIF
IGQSYHGVRAIANILTRNYGIEQHPLEDIEKCASEGDIVHLESPVNPYGTSSDIESLA
RRAHAKGALLIVDSTFASPPLQYAWNFGADIVLYSATKYFGGHSDLLSGVLVVKEEAT
SRQLKDDRIYLGTNVANLESFMLLRSLRTYEMRITKQSENATKLVRFLSDHQSEFDKV
LKTIYHSSLQTEEFVKKQLVGGYGPVFAITLYTKEQCKQLPLKLKYFHHATSLGGIES
LVEWRAMTDPYIDQTLIRVSVGCESANDLIKDLASALKELQDAA"
gene <336337..>337809
/gene="APE4"
/locus_tag="YHR113W"
/db_xref="GeneID:856513"
mRNA <336337..>337809
/gene="APE4"
/locus_tag="YHR113W"
/product="aspartyl aminopeptidase"
/transcript_id="NM_001179243.1"
/db_xref="GeneID:856513"
CDS 336337..337809
/gene="APE4"
/locus_tag="YHR113W"
/EC_number="3.4.11.21"
/experiment="EXISTENCE:direct assay:GO:0000328 fungal-type
vacuole lumen [PMID:21343297]"
/experiment="EXISTENCE:direct assay:GO:0004177
aminopeptidase activity [PMID:16367759]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0006508 proteolysis
[PMID:15606766]"
/note="Cytoplasmic aspartyl aminopeptidase with possible
vacuole function; targeted to vacuole via Vps10p-dependent
endosomal vacuolar protein sorting pathway and via CVT
pathway; cleaves unblocked N-terminal acidic amino acids
from peptide substrates; forms 12-subunit homo-oligomer;
M18 metalloprotease family"
/codon_start=1
/product="aspartyl aminopeptidase"
/protein_id="NP_011981.1"
/db_xref="GeneID:856513"
/db_xref="SGD:S000001155"
/translation="MFRIQLRTMSSKTCKSDYPKEFVSFLNSSHSPYHTVHNIKKHLV
SNGFKELSERDSWAGHVAQKGKYFVTRNGSSIIAFAVGGKWEPGNPIAITGAHTDSPA
LRIKPISKRVSEKYLQVGVETYGGAIWHSWFDKDLGVAGRVFVKDAKTGKSIARLVDL
NRPLLKIPTLAIHLDRDVNQKFEFNRETQLLPIGGLQEDKTEAKTEKEINNGEFTSIK
TIVQRHHAELLGLIAKELAIDTIEDIEDFELILYDHNASTLGGFNDEFVFSGRLDNLT
SCFTSMHGLTLAADTEIDRESGIRLMACFDHEEIGSSSAQGADSNFLPNILERLSILK
GDGSDQTKPLFHSAILETSAKSFFLSSDVAHAVHPNYANKYESQHKPLLGGGPVIKIN
ANQRYMTNSPGLVLVKRLAEAAKVPLQLFVVANDSPCGSTIGPILASKTGIRTLDLGN
PVLSMHSIRETGGSADLEFQIKLFKEFFERYTSIESEIVV"
gene <338083..>339984
/gene="BZZ1"
/locus_tag="YHR114W"
/gene_synonym="LSB7"
/db_xref="GeneID:856514"
mRNA <338083..>339984
/gene="BZZ1"
/locus_tag="YHR114W"
/gene_synonym="LSB7"
/product="Bzz1p"
/transcript_id="NM_001179244.1"
/db_xref="GeneID:856514"
CDS 338083..339984
/gene="BZZ1"
/locus_tag="YHR114W"
/gene_synonym="LSB7"
/experiment="EXISTENCE:direct assay:GO:0005543
phospholipid binding [PMID:25620000]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16824951]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0030479 actin
cortical patch [PMID:16824951|PMID:12391157]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807]"
/experiment="EXISTENCE:genetic interaction:GO:0006897
endocytosis [PMID:16231105]"
/experiment="EXISTENCE:genetic interaction:GO:0009651
response to salt stress [PMID:12391157]"
/experiment="EXISTENCE:mutant phenotype:GO:0008047 enzyme
activator activity [PMID:16824951]"
/experiment="EXISTENCE:mutant phenotype:GO:0045010 actin
nucleation [PMID:16824951]"
/experiment="EXISTENCE:physical interaction:GO:0007015
actin filament organization [PMID:12391157]"
/experiment="EXISTENCE:physical interaction:GO:0009651
response to salt stress [PMID:12391157]"
/note="SH3 domain protein implicated in regulating actin
polymerization; able to recruit actin polymerization
machinery through its SH3 domains; colocalizes with
cortical actin patches and Las17p; interacts with type I
myosins"
/codon_start=1
/product="Bzz1p"
/protein_id="NP_011982.1"
/db_xref="GeneID:856514"
/db_xref="SGD:S000001156"
/translation="MSADLSIGNEIKDSFKETHKWVQNNLKWLKDIEQFYRERAKLEK
DYSERLSRLSAEYFNKKSSTSVPISVGDTPTTTPGSIEAAGVVAWNEILSQTDMISKD
HDQLSTDFENHVANQLSGLFTKLDMTLSKINGFNNDMVNKKDNIYHELEKAKKDYDEA
CSTMEMARNRYTKASNDRNKKKLDEKEMEMNKCKNEYLIKINQANRTKDKYYFQDVPE
VLDLLQDVNEAKTLFLNDLWLKAASVENDLGANVSKRLQAANSVVKQNKPSLNTAIFI
KHNLKNWKEPQDFVYKPSPVWHDDEKFAVPSSLEVEDLRIKLAKAENDYNSLQDKTQN
ELSKLSTLNKIKHEMKTNEDNINATKFYDTLKEYLNVVSPFTSHETLKLQAEVQIESI
QNNVPEEYDLSTDNIDLSKTKKKSGIFSKFKHNILNVDSKPSSGGSTGNGNGGPLHIT
SLFNTSRRTRLGSAPNNAGEDSDNNSIRTTSTNNTKKTTQNSSDDGKNKVLYAYVQKD
DDEITITPGDKISLVARDTGSGWTKINNDTTGETGLVPTTYIRISSAATVKANDRGPA
PEVPPPRRSTLPVRTMEAIYAYEAQGDDEISIDPGDIITVIRGDDGSGWTYGECDGLK
GLFPTSYCK"
gene complement(<340109..>341359)
/gene="DMA1"
/locus_tag="YHR115C"
/gene_synonym="CHF1"
/db_xref="GeneID:856515"
mRNA complement(<340109..>341359)
/gene="DMA1"
/locus_tag="YHR115C"
/gene_synonym="CHF1"
/product="ubiquitin-conjugating protein DMA1"
/transcript_id="NM_001179245.1"
/db_xref="GeneID:856515"
CDS complement(340109..341359)
/gene="DMA1"
/locus_tag="YHR115C"
/gene_synonym="CHF1"
/EC_number="2.3.2.27"
/experiment="EXISTENCE:direct assay:GO:0004842
ubiquitin-protein transferase activity [PMID:18202552]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0016567 protein
ubiquitination [PMID:23264631]"
/experiment="EXISTENCE:direct assay:GO:0051865 protein
autoubiquitination [PMID:18202552]"
/experiment="EXISTENCE:genetic interaction:GO:0000132
establishment of mitotic spindle orientation
[PMID:22570619|PMID:15146058]"
/experiment="EXISTENCE:genetic interaction:GO:0000921
septin ring assembly [PMID:15146058|PMID:22570619]"
/experiment="EXISTENCE:genetic interaction:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:21562220]"
/experiment="EXISTENCE:genetic interaction:GO:0031578
mitotic spindle orientation checkpoint signaling
[PMID:15146058|PMID:22570619]"
/experiment="EXISTENCE:genetic interaction:GO:0032186
cellular bud neck septin ring organization
[PMID:22570619]"
/experiment="EXISTENCE:genetic interaction:GO:0097271
protein localization to bud neck [PMID:22570619]"
/experiment="EXISTENCE:mutant phenotype:GO:0004842
ubiquitin-protein transferase activity [PMID:18202552]"
/experiment="EXISTENCE:mutant phenotype:GO:0090337
regulation of formin-nucleated actin cable assembly
[PMID:27449057]"
/experiment="EXISTENCE:physical interaction:GO:0090337
regulation of formin-nucleated actin cable assembly
[PMID:27449057]"
/note="Ubiquitin-protein ligase (E3); controls septin
dynamics, spindle position checkpoint (SPOC) with ligase
Dma2p by regulating recruitment of Elm1p to bud neck;
regulates levels of eIF2 subunit Gcd11p, as well as
abundance, localization, and ubiquitination of Cdk
inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p;
ortholog of human RNF8, similar to human Chfr; contains
FHA, RING fingers; DMA1 has a paralog, DMA2, that arose
from the whole genome duplication"
/codon_start=1
/product="ubiquitin-conjugating protein DMA1"
/protein_id="NP_011983.1"
/db_xref="GeneID:856515"
/db_xref="SGD:S000001157"
/translation="MSTNTVPSSPPNQTPPAASGIATSHDHTKFNNPIRLPISISLTI
NDTPNNNSNNNSVSNGLGILPSRTATSLVVANNGSANGNVGATAAAAATVETNTAPAV
NTTKSIRHFIYPPNQVNQTEFSLDIHLPPNTSLPERIDQSTLKRRMDKHGLFSIRLTP
FIDTSSTSVANQGLFFDPIIRTAGAGSQIIIGRYTERVREAISKIPDQYHPVVFKSKV
ISRTHGCFKVDDQGNWFLKDVKSSSGTFLNHQRLSSASTTSKDYLLHDGDIIQLGMDF
RGGTEEIYRCVKMKIELNKSWKLKANAFNKEALSRIKNLQKLTTGLEQEDCSICLNKI
KPCQAIFISPCAHSWHFHCVRRLVIMNYPQFMCPNCRTNCDLETTLESESESEFENED
EDEPDIEMDIDMEINNNLGVRLVD"
gene <341665..>342120
/gene="COX23"
/locus_tag="YHR116W"
/db_xref="GeneID:856516"
mRNA <341665..>342120
/gene="COX23"
/locus_tag="YHR116W"
/product="Cox23p"
/transcript_id="NM_001179246.1"
/db_xref="GeneID:856516"
CDS 341665..342120
/gene="COX23"
/locus_tag="YHR116W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:15145942]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:15145942]"
/experiment="EXISTENCE:mutant phenotype:GO:0033108
mitochondrial respiratory chain complex assembly
[PMID:19703468]"
/note="Protein that functions in mitochondrial copper
homeostasis; mitochondrial intermembrane space protein;
essential for functional cytochrome oxidase expression;
homologous to Cox17p; contains twin cysteine-x9-cysteine
motifs"
/codon_start=1
/product="Cox23p"
/protein_id="NP_011984.1"
/db_xref="GeneID:856516"
/db_xref="SGD:S000001158"
/translation="MEKPSPTRRQTSSLSTISNGMTMTNDNRDTTNTNSGSTSSNNSQ
PSSSSTPPAASGPVTDRTKVNYVPKSDDPSSFQYYPDDPENPVNKYKFALKADSQYYD
PCEESSKLSFQCLERNDYDRSKCQEYFDAYRECKKQWLTARRKNRQQWE"
gene <342349..>344268
/gene="TOM71"
/locus_tag="YHR117W"
/gene_synonym="TOM72"
/db_xref="GeneID:856517"
mRNA <342349..>344268
/gene="TOM71"
/locus_tag="YHR117W"
/gene_synonym="TOM72"
/product="protein channel TOM71"
/transcript_id="NM_001179247.1"
/db_xref="GeneID:856517"
CDS 342349..344268
/gene="TOM71"
/locus_tag="YHR117W"
/gene_synonym="TOM72"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane
[PMID:16407407|PMID:16689936|PMID:8663394]"
/experiment="EXISTENCE:genetic interaction:GO:0006626
protein targeting to mitochondrion [PMID:18007655]"
/experiment="EXISTENCE:mutant phenotype:GO:0006626 protein
targeting to mitochondrion [PMID:18007655]"
/note="Mitochondrial outer membrane protein; probable
minor component of the TOM (translocase of outer membrane)
complex responsible for recognition and import of
mitochondrially directed proteins; unique role in the dual
distribution of tail-anchored proteins between
mitochondria and peroxisomes; TOM71 has a paralog, TOM70,
that arose from the whole genome duplication"
/codon_start=1
/product="protein channel TOM71"
/protein_id="NP_011985.1"
/db_xref="GeneID:856517"
/db_xref="SGD:S000001159"
/translation="MAENSLLRFITKNKVAILATVSAGTAAVGAYVYYQQIKQQQQQQ
LKGTKDNRRQSEAFAGQNEDEADLKDDGSVVSGSNKRKKKKNKRKRNNKAKSGEGFDY
PSLPNGEPDIAQLKGLSPSQRQAYAVQLKNRGNHFFTAKNFNEAIKYYQYAIELDPNE
PVFYSNISACYISTGDLEKVIEFTTKALEIKPDHSKALLRRASANESLGNFTDAMFDL
SVLSLNGDFDGASIEPMLERNLNKQAMKVLNENLSKDEGRGSQVLPSNTSLASFFGIF
DSHLEVSSVNTSSNYDTAYALLSDALQRLYSATDEGYLVANDLLTKSTDMYHSLLSAN
TVDDPLRENAALALCYTGIFHFLKNNLLDAQVLLQESINLHPTPNSYIFLALTLADKE
NSQEFFKFFQKAVDLNPEYPPTYYHRGQMYFILQDYKNAKEDFQKAQSLNPENVYPYI
QLACLLYKQGKFTESEAFFNETKLKFPTLPEVPTFFAEILTDRGDFDTAIKQYDIAKR
LEEVQEKIHVGIGPLIGKATILARQSSQDPTQLDEEKFNAAIKLLTKACELDPRSEQA
KIGLAQLKLQMEKIDEAIELFEDSAILARTMDEKLQATTFAEAAKIQKRLRADPIISA
KMELTLARYRAKGML"
gene complement(<344321..>345628)
/gene="ORC6"
/locus_tag="YHR118C"
/db_xref="GeneID:856518"
mRNA complement(<344321..>345628)
/gene="ORC6"
/locus_tag="YHR118C"
/product="origin recognition complex subunit 6"
/transcript_id="NM_001179248.1"
/db_xref="GeneID:856518"
CDS complement(344321..345628)
/gene="ORC6"
/locus_tag="YHR118C"
/experiment="EXISTENCE:direct assay:GO:0003688 DNA
replication origin binding [PMID:16824194]"
/experiment="EXISTENCE:direct assay:GO:0005656 nuclear
pre-replicative complex [PMID:9335335|PMID:16824194]"
/experiment="EXISTENCE:direct assay:GO:0005664 nuclear
origin of replication recognition complex [PMID:9372948]"
/experiment="EXISTENCE:direct assay:GO:0006267
pre-replicative complex assembly involved in nuclear cell
cycle DNA replication [PMID:16824194]"
/experiment="EXISTENCE:direct assay:GO:0030466 silent
mating-type cassette heterochromatin formation
[PMID:12897051]"
/experiment="EXISTENCE:direct assay:GO:0031261 DNA
replication preinitiation complex [PMID:9554851]"
/experiment="EXISTENCE:mutant phenotype:GO:0005664 nuclear
origin of replication recognition complex [PMID:9372948]"
/experiment="EXISTENCE:mutant phenotype:GO:0006270 DNA
replication initiation [PMID:17053779]"
/note="Subunit of the origin recognition complex (ORC);
ORC directs DNA replication by binding to replication
origins and is also involved in transcriptional silencing;
phosphorylated by Cdc28p; mutation in the human Orc6p is
linked to Meier-Gorlin syndrome"
/codon_start=1
/product="origin recognition complex subunit 6"
/protein_id="NP_011986.1"
/db_xref="GeneID:856518"
/db_xref="SGD:S000001160"
/translation="MSMQQVQHCVAEVLRLDPQEKPDWSSGYLKKLTNATSILYNTSL
NKVMLKQDEEVARCHICAYIASQKMNEKHMPDLCYYIDSIPLEPKKAKHLMNLFRQSL
SNSSPMKQFAWTPSPKKNKRSPVKNGGRFTSSDPKELRNQLFGTPTKVRKSQNNDSFV
IPELPPMQTNESPSITRRKLAFEEDEDEDEEEPGNDGLSLKSHSNKSITGTRNVDSDE
YENHESDPTSEEEPLGVQESRSGRTKQNKAVGKPQSELKTAKALRKRGRIPNSLLVKK
YCKMTTEEIIRLCNDFELPREVAYKIVDEYNINASRLVCPWQLVCGLVLNCTFIVFNE
RRRKDPRIDHFIVSKMCSLMLTSKVDDVIECVKLVKELIIGEKWFRDLQIRYDDFDGI
RYDEIIFRKLGSMLQTTNILVTDDQYNIWKKRIEMDLALTEPL"
gene <346043..>349285
/gene="SET1"
/locus_tag="YHR119W"
/gene_synonym="KMT2; YTX1"
/db_xref="GeneID:856519"
mRNA <346043..>349285
/gene="SET1"
/locus_tag="YHR119W"
/gene_synonym="KMT2; YTX1"
/product="histone methyltransferase SET1"
/transcript_id="NM_001179249.1"
/db_xref="GeneID:856519"
CDS 346043..349285
/gene="SET1"
/locus_tag="YHR119W"
/gene_synonym="KMT2; YTX1"
/EC_number="2.1.1.354"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:17898715]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:28483910|PMID:16787775]"
/experiment="EXISTENCE:direct assay:GO:0042800 histone
H3K4 methyltransferase activity
[PMID:11805083|PMID:11742990]"
/experiment="EXISTENCE:direct assay:GO:0045815
transcription initiation-coupled chromatin remodeling
[PMID:17898715]"
/experiment="EXISTENCE:direct assay:GO:0140946 histone
H3K4 dimethyltransferase activity [PMID:17898715]"
/experiment="EXISTENCE:direct assay:GO:0140999 histone
H3K4 trimethyltransferase activity [PMID:17898715]"
/experiment="EXISTENCE:genetic interaction:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:16959218]"
/experiment="EXISTENCE:genetic interaction:GO:0016279
protein-lysine N-methyltransferase activity
[PMID:16143104]"
/experiment="EXISTENCE:genetic interaction:GO:0033554
cellular response to stress [PMID:16959218]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:27325136]"
/experiment="EXISTENCE:mutant phenotype:GO:0000723
telomere maintenance
[PMID:27911222|PMID:9398665|PMID:11742990]"
/experiment="EXISTENCE:mutant phenotype:GO:0003723 RNA
binding [PMID:16787775]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:16959218]"
/experiment="EXISTENCE:mutant phenotype:GO:0016279
protein-lysine N-methyltransferase activity
[PMID:16143104]"
/experiment="EXISTENCE:mutant phenotype:GO:0030437
ascospore formation [PMID:17150765]"
/experiment="EXISTENCE:mutant phenotype:GO:0030466 silent
mating-type cassette heterochromatin formation
[PMID:9398665]"
/experiment="EXISTENCE:mutant phenotype:GO:0031509
subtelomeric heterochromatin formation
[PMID:9398665|PMID:9988274|PMID:11752412|PMID:11805083]"
/experiment="EXISTENCE:mutant phenotype:GO:0033554
cellular response to stress [PMID:16959218]"
/experiment="EXISTENCE:mutant phenotype:GO:0042138 meiotic
DNA double-strand break formation [PMID:32290544]"
/experiment="EXISTENCE:mutant phenotype:GO:0042800 histone
H3K4 methyltransferase activity
[PMID:12845608|PMID:11752412|PMID:11742990|PMID:11805083]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:27325136]"
/experiment="EXISTENCE:mutant phenotype:GO:1902275
regulation of chromatin organization [PMID:27325136]"
/experiment="EXISTENCE:mutant phenotype:GO:1903341
regulation of meiotic DNA double-strand break formation
[PMID:23246437]"
/experiment="EXISTENCE:physical interaction:GO:0048188
Set1C/COMPASS complex
[PMID:11742990|PMID:11752412|PMID:11687631]"
/note="Histone methyltransferase, subunit of the COMPASS
(Set1C) complex; COMPASS methylates histone H3K4;
Set1p-dependent H3K4 trimethylation recruits Nrd1p,
allowing efficient termination of snoRNAs and cryptic
unstable transcripts (CUTs) by Nrd1p-Nab3p-Sen1p pathway;
modulates histone acetylation levels in promoter proximal
regions to ensure efficient Nrd1p-dependent termination;
required in transcriptional silencing near telomeres and
at silent mating type loci; has a SET domain"
/codon_start=1
/product="histone methyltransferase SET1"
/protein_id="NP_011987.1"
/db_xref="GeneID:856519"
/db_xref="SGD:S000001161"
/translation="MSNYYRRAHASSGSYRQPQEQPQYSRSGHYQYSNGHSHQQYSSQ
YNQRRRYNHNDGTRRRYNDDRPHSSNNASTRQYYATNNSQSGPYVNKKSDISSRRGMS
QSRYSNSNVHNTLASSSGSLPTESALLLQQRPPSVLRYNTDNLKSKFHYFDPIKGEFF
NKDKMLSWKATDKEFSETGYYVVKELQDGQFKFKIKHRHPEIKASDPRNENGIMTSGK
VATHRKCRNSLILLPRISYDRYSLGPPPSCEIVVYPAQDSTTTNIQDISIKNYFKKYG
EISHFEAFNDPNSALPLHVYLIKYASSDGKINDAAKAAFSAVRKHESSGCFIMGFKFE
VILNKHSILNNIISKFVEINVKKLQKLQENLKKAKEKEAENEKAKELQGKDITLPKEP
KVDTLSHSSGSEKRIPYDLLGVVNNRPVLHVSKIFVAKHRFCVEDFKYKLRGYRCAKF
IDHPTGIYIIFNDIAHAQTCSNAESGNLTIMSRSRRIPILIKFHLILPRFQNRTRFNK
SSSSSNSTNVPIKYESKEEFIEATAKQILKDLEKTLHVDIKKRLIGPTVFDALDHANF
PELLAKRELKEKEKRQQIASKIAEDELKRKEEAKRDFDLFGLYGGYAKSNKRNLKRHN
SLALDHTSLKRKKLSNGIKPMAHLLNEETDSKETTPLNDEGITRVSKEHDEEDENMTS
SSSEEEEEEAPDKKFKSESEPTTPESDHLHGIKPLVPDQNGSSDVLDASSMYKPTATE
IPEPVYPPEEYDLKYSQTLSSMDLQNAIKDEEDMLILKQLLSTYTPTVTPETSAALEY
KIWQSRRKVLEEEKASDWQIELNGTLFDSELQPGSSFKAEGFRKIADKLKINYLPHRR
RVHQPLNTVNIHNERNEYTPELCQREESSNKEPSDSVPQEVSSSRDNRASNRRFQQDI
EAQKAAIGTESELLSLNQLNKRKKPVMFARSAIHNWGLYALDSIAAKEMIIEYVGERI
RQPVAEMREKRYLKNGIGSSYLFRVDENTVIDATKKGGIARFINHCCDPNCTAKIIKV
GGRRRIVIYALRDIAASEELTYDYKFEREKDDEERLPCLCGAPNCKGFLN"
gene <349574..>352453
/gene="MSH1"
/locus_tag="YHR120W"
/db_xref="GeneID:856520"
mRNA <349574..>352453
/gene="MSH1"
/locus_tag="YHR120W"
/product="mismatch repair ATPase MSH1"
/transcript_id="NM_001179250.1"
/db_xref="GeneID:856520"
CDS 349574..352453
/gene="MSH1"
/locus_tag="YHR120W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:24769239|PMID:15611870|PMID:14576278|PMID:7961998|PM
ID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0008094
ATP-dependent activity, acting on DNA [PMID:7961999]"
/experiment="EXISTENCE:direct assay:GO:0032137
guanine/thymine mispair binding [PMID:7961998]"
/experiment="EXISTENCE:direct assay:GO:0032139
dinucleotide insertion or deletion binding [PMID:7961998]"
/experiment="EXISTENCE:mutant phenotype:GO:0006281 DNA
repair [PMID:1334021]"
/experiment="EXISTENCE:mutant phenotype:GO:0006298
mismatch repair [PMID:15611870]"
/experiment="EXISTENCE:mutant phenotype:GO:0007005
mitochondrion organization [PMID:1334021|PMID:15611870]"
/experiment="EXISTENCE:mutant phenotype:GO:0043504
mitochondrial DNA repair [PMID:1334021]"
/note="DNA-binding protein of the mitochondria; involved
in repair of mitochondrial DNA; has ATPase activity and
binds to DNA mismatches; has homology to E. coli MutS;
transcription is induced during meiosis"
/codon_start=1
/product="mismatch repair ATPase MSH1"
/protein_id="NP_011988.1"
/db_xref="GeneID:856520"
/db_xref="SGD:S000001162"
/translation="MKHFFRLPTAFRPISRVSLRYSSTDTAQPKISKLKISFNKISES
NSEKKDNLGSIDTRNCLSTQQDDKLSSTEPSKASLPPSLQYVRDLMDLYKDHVVLTQM
GSFYELYFEQAIRYAPELNISLTNRAYSHGKVPFAGFPVHQLSRHLKMLVNNCGYSVT
IAEQFKKKDVADNEANKFYRRVTRIVTPGTFIDEAFENLRENTYLLNIEFPENCMSQV
ADTSLKVGICWCDVSTGEIFVQQVYLRDLVSAITRIQPKEILLDERLLEFHIESGTWY
PELVELKKFFIKYQKMPSQHRTIESFYGLFNLGGKEATERQLKIQFQTFTQKELAALR
NTLIYVSNHLPDFSINFQIPQRQLATAIMQIDSRTSTALELHSTVRDNNKKGSLLSSI
RRTVTPSGTRLLSQWLSGPSLDLKEIKKRQKIVAFFKDNRDITETLRTMLKKVNDLSR
ILQKFSFGRGEALELIQMARSLEVSREIRKYLLNNTSLMKATLKSQITQLTESLNFEK
NLIDDILKFLNEEELAKSQDAKQNADVTRMLDIDVKDKKESNKDEIFELRDFIVNPSF
NTKLRKLHDTYQGVWQKKTEYNALLKGFFVGDLGAKTFTLKERQNGEYALHVTGTASS
LKKIDELISKSTEYHGSCFHILQKSSQTRWLSHKIWTDLGHELELLNLKIRNEEANII
DLFKRKFIDRSNEVRQVATTLGYLDTLSSFAVLANERNLVCPKVDESNKLEVVNGRHL
MVEEGLSARSLETFTANNCELAKDNLWVITGPNMGGKSTFLRQNAIIVILAQIGCFVP
CSKARVGIVDKLFSRVGSADDLYNEMSTFMVEMIETSFILQGATERSLAILDEIGRGT
SGKEGISIAYATLKYLLENNQCRTLFATHFGQELKQIIDNKCSKGMSEKVKFYQSGIT
DLGGNNFCYNHKLKPGICTKSDAIRVAELAGFPMEALKEAREILG"
gene <352756..>353319
/gene="LSM12"
/locus_tag="YHR121W"
/db_xref="GeneID:856521"
mRNA <352756..>353319
/gene="LSM12"
/locus_tag="YHR121W"
/product="Lsm12p"
/transcript_id="NM_001179251.1"
/db_xref="GeneID:856521"
CDS 352756..353319
/gene="LSM12"
/locus_tag="YHR121W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405|PMID:20368989]"
/experiment="EXISTENCE:physical interaction:GO:0016070 RNA
metabolic process [PMID:16702403]"
/note="hypothetical protein that may function in RNA
processing; interacts with Pbp1p and Pbp4p and associates
with ribosomes; contains an RNA-binding LSM domain and an
AD domain; GFP-fusion protein is induced by the
DNA-damaging agent MMS; relative distribution to the
nucleus increases upon DNA replication stress"
/codon_start=1
/product="Lsm12p"
/protein_id="NP_011989.1"
/db_xref="GeneID:856521"
/db_xref="SGD:S000001163"
/translation="MSVSLEQTLGFRIKVTNVLDVVTEGRLYSFNSSNNTLTIQTTKK
NQSPQNFKVIKCTFIKHLEVIGDKPSFNSFKKQQIKPSYVNVERVEKLLKESVIASKK
KELLRGKGVSAEGQFIFDQIFKTIGDTKWVAKDIIILDDVKVQPPYKVEDIKVLHEGS
NQSITLIQRIVERSWEQLEQDDGRKGG"
gene <353625..>354320
/gene="CIA2"
/locus_tag="YHR122W"
/db_xref="GeneID:856522"
mRNA <353625..>354320
/gene="CIA2"
/locus_tag="YHR122W"
/product="iron-sulfur cluster assembly protein CIA2"
/transcript_id="NM_001179252.1"
/db_xref="GeneID:856522"
CDS 353625..354320
/gene="CIA2"
/locus_tag="YHR122W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14690591]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14690591|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0097361 cytosolic
[4Fe-4S] assembly targeting complex [PMID:22678362]"
/experiment="EXISTENCE:mutant phenotype:GO:0016226
iron-sulfur cluster assembly [PMID:22678362]"
/note="Component of cytosolic iron-sulfur protein assembly
(CIA) machinery; acts at a late step of Fe-S cluster
assembly; forms the CIA targeting complex with Cia1p and
Met18p that directs Fe-S cluster incorporation and
maturation of a subset of proteins involved in methionine
biosynthesis, DNA replication and repair, transcription,
and telomere maintenance; ortholog of human FAM96B"
/codon_start=1
/product="iron-sulfur cluster assembly protein CIA2"
/protein_id="NP_011990.1"
/db_xref="GeneID:856522"
/db_xref="SGD:S000001164"
/translation="MSEFLNENPDILEENQLPTRKEDSTKDLLLGGFSNEATLERRSL
LLKIDHSLKSQVLQDIEVLDKLLSIRIPPELTSDEDSLPAESEDESVAGGGKEEEEPD
LIDAQEIYDLIAHISDPEHPLSLGQLSVVNLEDIDVHDSGNQNEMAEVVIKITPTITH
CSLATLIGLGIRVRLERSLPPRFRITILLKKGTHDSENQVNKQLNDKERVAAACENEQ
LLGVVSKMLVTCK"
gene <354815..>356081
/gene="EPT1"
/locus_tag="YHR123W"
/db_xref="GeneID:856523"
mRNA join(<354815..354864,354956..>356081)
/gene="EPT1"
/locus_tag="YHR123W"
/product="bifunctional diacylglycerol
cholinephosphotransferase/ethanolaminephosphotransferase"
/transcript_id="NM_001179253.1"
/db_xref="GeneID:856523"
CDS join(354815..354864,354956..356081)
/gene="EPT1"
/locus_tag="YHR123W"
/EC_number="2.7.8.1"
/EC_number="2.7.8.2"
/experiment="EXISTENCE:direct assay:GO:0004142
diacylglycerol cholinephosphotransferase activity
[PMID:2848840]"
/experiment="EXISTENCE:direct assay:GO:0004307
ethanolaminephosphotransferase activity [PMID:2848840]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006646
phosphatidylethanolamine biosynthetic process
[PMID:2848840]"
/experiment="EXISTENCE:direct assay:GO:0006656
phosphatidylcholine biosynthetic process [PMID:2848840]"
/experiment="EXISTENCE:mutant phenotype:GO:0004142
diacylglycerol cholinephosphotransferase activity
[PMID:2848840]"
/experiment="EXISTENCE:mutant phenotype:GO:0004307
ethanolaminephosphotransferase activity [PMID:2848840]"
/experiment="EXISTENCE:mutant phenotype:GO:0006646
phosphatidylethanolamine biosynthetic process
[PMID:2848840]"
/experiment="EXISTENCE:mutant phenotype:GO:0006656
phosphatidylcholine biosynthetic process [PMID:2848840]"
/note="sn-1,2-diacylglycerol ethanolamine- and
cholinephosphotranferase; not essential for viability;
EPT1 has a paralog, CPT1, that arose from the whole genome
duplication"
/codon_start=1
/product="bifunctional diacylglycerol
cholinephosphotransferase/ethanolaminephosphotransferase"
/protein_id="NP_011991.1"
/db_xref="GeneID:856523"
/db_xref="SGD:S000001165"
/translation="MGYFVPDSHIENLKSYKYQSEDRSLVSKYFLKPFWQRFCHIFPT
WMAPNIITLSGFAFIVINVLTVFYYDPNLNTDTPRWTYFSYALGVFLYQTFDGCDGVH
ARRINQSGPLGELFDHSIDAINSTLSIFIFASETGMGFSYNLMLSQFAMLTNFYLSTW
EEYHTHTLYLSEFSGPVEGILIVCVSLILTGIYGKQVIWHTYLFTITVGDKVIDVDTL
DIVFSLAVFGLVMNALSAKRNVDKYYRNSTSSANNITQIEQDSAIKGLLPFFAYYASI
ALLVWMQPSFITLSFILSVGFTGAFTVGRIIVCHLTKQSFPMFNAPMLIPLCQIVLYK
ICLSLWGIESNKIVFALSWLGFGLSLGVHIMFMNDIIHEFTEYLDVYALSIKRSKLT"
gene <356561..>358444
/gene="NDT80"
/locus_tag="YHR124W"
/db_xref="GeneID:856524"
mRNA <356561..>358444
/gene="NDT80"
/locus_tag="YHR124W"
/product="transcription factor NDT80"
/transcript_id="NM_001179254.1"
/db_xref="GeneID:856524"
CDS 356561..358444
/gene="NDT80"
/locus_tag="YHR124W"
/experiment="EXISTENCE:direct assay:GO:0000228 nuclear
chromosome [PMID:11035815]"
/experiment="EXISTENCE:direct assay:GO:0003700 DNA-binding
transcription factor activity [PMID:9660952]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:19158363]"
/experiment="EXISTENCE:mutant phenotype:GO:0003700
DNA-binding transcription factor activity [PMID:9660952]"
/experiment="EXISTENCE:mutant phenotype:GO:0043934
sporulation [PMID:9660952]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:9660952]"
/experiment="EXISTENCE:mutant phenotype:GO:0051321 meiotic
cell cycle [PMID:8524222]"
/note="Meiosis-specific transcription factor; required for
exit from pachytene and for full meiotic recombination;
activates middle sporulation genes; competes with Sum1p
for binding to promoters containing middle sporulation
elements (MSE)"
/codon_start=1
/product="transcription factor NDT80"
/protein_id="NP_011992.1"
/db_xref="GeneID:856524"
/db_xref="SGD:S000001166"
/translation="MNEMENTDPVLQDDLVSKYERELSTEQEEDTPVILTQLNEDGTT
SNYFDKRKLKIAPRSTLQFKVGPPFELVRDYCPVVESHTGRTLDLRIIPRIDRGFDHI
DEEWVGYKRNYFTLVSTFETANCDLDTFLKSSFDLLVEDSSVESRLRVQYFAIKIKAK
NDDDDTEINLVQHTAKRDKGPQFCPSVCPLVPSPLPKHQIIREASNVRNITKMKKYDS
TFYLHRDHVNYEEYGVDSLLFSYPEDSIQKVARYERVQFASSISVKKPSQQNKHFSLH
VILGAVVDPDTFHGENPGIPYDELALKNGSKGMFVYLQEMKTPPLIIRGRSPSNYASS
QRITVRTPSSVNSSQNSTKRKMPSMAQPLNESCLNARPSKRRSKVALGAPNSGASISP
IKSRQSTPMEASKENEDPFFRPNKRVETLEHIQNKLGALKNQCPDSSLKYPSSSSRGM
EGCLEKEDLVYSSSFSVNMKQIELKPARSFEHENIFKVGSLAFKKINELPHENYDITI
EKKSMEQNYLRPEIGSRSECKTSYGNELSLSNISFSILPNSAENFHLETALFPATEED
VPRTFSRILETGSFQNYYQKMDAENADRVYSKGVKLIASGTLPSGIFNREELFEEDSF
YKY"
gene complement(358478..358569)
/locus_tag="YNCH0010C"
/db_xref="GeneID:856525"
tRNA complement(join(358478..358513,358533..358569))
/locus_tag="YNCH0010C"
/product="tRNA-Phe"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:10024171]"
/note="Phenylalanine tRNA (tRNA-Phe), predicted by
tRNAscan-SE analysis"
/db_xref="GeneID:856525"
/db_xref="SGD:S000006567"
repeat_region complement(358672..358850)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006996"
repeat_region complement(358850..359067)
/note="Ty4 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007005"
gene <358858..>359163
/locus_tag="YHR125W"
/db_xref="GeneID:856526"
mRNA <358858..>359163
/locus_tag="YHR125W"
/product="uncharacterized protein"
/transcript_id="NM_001348824.1"
/db_xref="GeneID:856526"
CDS 358858..359163
/locus_tag="YHR125W"
/note="hypothetical protein; conserved across S.
cerevisiae strains"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335740.1"
/db_xref="GeneID:856526"
/db_xref="SGD:S000001167"
/translation="MINAIMIGSCQTFNTILCNISMNNYHSNVQLSSIRLQYCMKYSL
TFSLFYNSTVTLKRQTWRTLIYDVCIWLDTAVIRNIIIRQLVVTLVVYYHMVCKNIT"
repeat_region 359079..359372
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007008"
gene complement(<359702..>360181)
/gene="ANS1"
/locus_tag="YHR126C"
/db_xref="GeneID:856527"
mRNA complement(<359702..>360181)
/gene="ANS1"
/locus_tag="YHR126C"
/product="Ans1p"
/transcript_id="NM_001179256.1"
/db_xref="GeneID:856527"
CDS complement(359702..360181)
/gene="ANS1"
/locus_tag="YHR126C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/note="Putative GPI protein; SWAT-GFP and mCherry fusion
proteins localize to the vacuole; transcription dependent
upon Azf1p"
/codon_start=1
/product="Ans1p"
/protein_id="NP_011994.1"
/db_xref="GeneID:856527"
/db_xref="SGD:S000001168"
/translation="MKCTLVSTLFAITNILVAHAQVSNSSDTLDVQFANSTNSYIEGK
FNSTDEAFNSSASWSLAAQQKKISNAAVYDVGGWNGSLYRSNRSAVADHQPGKKQDAA
ISQISDGQIQATASGPETTAATTPSSTANVSVYEGAGMKVESKNMGYIVGVAALLFL"
gene <360913..>361644
/gene="YHS7"
/locus_tag="YHR127W"
/db_xref="GeneID:856528"
mRNA <360913..>361644
/gene="YHS7"
/locus_tag="YHR127W"
/product="mRNP network protein YHS7"
/transcript_id="NM_001179257.1"
/db_xref="GeneID:856528"
CDS 360913..361644
/gene="YHS7"
/locus_tag="YHR127W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10684247]"
/experiment="EXISTENCE:mutant phenotype:GO:0000022 mitotic
spindle elongation [PMID:18194531]"
/note="mRNP network protein; pioneering mRNP component
functioning early in mRNP assembly and packaging, along
with Yra1p to which it is in proximity within mRNPs; has
capacity to promote RNA-RNA interactions via positively
charged IDR; localizes to the nucleus; required for
asymmetric localization of Kar9p during mitosis; has
structured domains that can engage in physical
interactions when brought in close proximity in mRNP
condensation process"
/codon_start=1
/product="mRNP network protein YHS7"
/protein_id="NP_011995.1"
/db_xref="GeneID:856528"
/db_xref="SGD:S000001169"
/translation="MARNRTTSKKNVQSKRLIDRVVPMDKIKKVGVAKKKTVEHTKEG
FSVVNGKLVSSNDVGVLLREAQGAIDKRTNVSQRNRKKGIKNNRPHKDINSSPDWGNA
HRGTDWQSEKANGMNRAKNSRNFTTNIKLQRQHFGEEIQGGSQLVISTNSDASDKLLM
LFNLTLGVNQENLKNVLENISQVQIAQIRVRDLPSGSATAKVRLAYPTTQSLEKVRKL
FHGALVDGRRIQVVIASDESSHLSY"
gene <362115..>362765
/gene="FUR1"
/locus_tag="YHR128W"
/db_xref="GeneID:856529"
mRNA <362115..>362765
/gene="FUR1"
/locus_tag="YHR128W"
/product="uracil phosphoribosyltransferase"
/transcript_id="NM_001179258.1"
/db_xref="GeneID:856529"
CDS 362115..362765
/gene="FUR1"
/locus_tag="YHR128W"
/EC_number="2.4.2.9"
/experiment="EXISTENCE:curator inference:GO:0005737
cytoplasm [PMID:2189783]"
/experiment="EXISTENCE:direct assay:GO:0004845 uracil
phosphoribosyltransferase activity [PMID:2189783]"
/experiment="EXISTENCE:direct assay:GO:0008655
pyrimidine-containing compound salvage [PMID:2189783]"
/note="Uracil phosphoribosyltransferase; synthesizes UMP
from uracil; involved in the pyrimidine salvage pathway"
/codon_start=1
/product="uracil phosphoribosyltransferase"
/protein_id="NP_011996.2"
/db_xref="GeneID:856529"
/db_xref="SGD:S000001170"
/translation="MSSEPFKNVYLLPQTNQLLGLYTIIRNKNTTRPDFIFYSDRIIR
LLVEEGLNHLPVQKQIVETDTNENFEGVSFMGKICGVSIVRAGESMEQGLRDCCRSVR
IGKILIQRDEETALPKLFYEKLPEDISERYVFLLDPMLATGGSAIMATEVLIKRGVKP
ERIYFLNLICSKEGIEKYHAAFPEVRIVTGALDRGLDENKYLVPGLGDFGDRYYCV"
gene complement(<362999..>364153)
/gene="ARP1"
/locus_tag="YHR129C"
/gene_synonym="ACT5"
/db_xref="GeneID:856530"
mRNA complement(<362999..>364153)
/gene="ARP1"
/locus_tag="YHR129C"
/gene_synonym="ACT5"
/product="actin-related protein 1"
/transcript_id="NM_001179259.1"
/db_xref="GeneID:856530"
CDS complement(362999..364153)
/gene="ARP1"
/locus_tag="YHR129C"
/gene_synonym="ACT5"
/experiment="EXISTENCE:direct assay:GO:0005200 structural
constituent of cytoskeleton [PMID:9658168]"
/experiment="EXISTENCE:direct assay:GO:0005869 dynactin
complex [PMID:9658168]"
/experiment="EXISTENCE:genetic interaction:GO:0031578
mitotic spindle orientation checkpoint signaling
[PMID:11285282]"
/experiment="EXISTENCE:mutant phenotype:GO:0000132
establishment of mitotic spindle orientation
[PMID:8069915]"
/experiment="EXISTENCE:mutant phenotype:GO:0007097 nuclear
migration [PMID:8069915]"
/note="Actin-related protein of the dynactin complex;
required for spindle orientation and nuclear migration;
forms actin-like short filament composed of 9 or 10 Arp1p
monomers; putative ortholog of mammalian centractin"
/codon_start=1
/product="actin-related protein 1"
/protein_id="NP_011997.1"
/db_xref="GeneID:856530"
/db_xref="SGD:S000001171"
/translation="MDQLSDSYALYNQPVVIDNGSGIIKAGFSGEERPKALEYCLVGN
TKYDKVMLEGLQGDTFIGNNAQKLRGLLKLRYPIKHGVVEDWDSMELIWSYVLNEVLQ
LQNIGEHPLLITEAPMNPLKNREQMAQVLFETFDVSALYVSNPAVLSLYASGRTTGCV
VDCGEGYCSTVPIYDGFALPASMMRMDIGGADITEQLQFQLRKSAGVSLFSSSEREIV
RTMKEKVCYLAKNIKKEEEKYLQGTQDLISTFKLPDGRCIEVGNDRYRAPEILFSPQI
IGLGYDGLSDMCMQSIWKVDLDLRKPLLSSIILSGGTTTLKGFGDRMLWDLEALTKGT
SKIKIIAPSERKYTTWIGGSILTGLSTFQRLWTKKSDWLEDSTRVYSNLM"
gene complement(<364965..>365300)
/locus_tag="YHR130C"
/db_xref="GeneID:856531"
mRNA complement(<364965..>365300)
/locus_tag="YHR130C"
/product="uncharacterized protein"
/transcript_id="NM_001348825.1"
/db_xref="GeneID:856531"
CDS complement(364965..365300)
/locus_tag="YHR130C"
/note="hypothetical protein; conserved among S. cerevisiae
strains; YHR130C is not an essential gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335741.1"
/db_xref="GeneID:856531"
/db_xref="SGD:S000001172"
/translation="MTKSIYIIIGYMLHDEEFFYFFFISFYTLWIVFFLLHLSFFSTL
SFGIFHDFDTDVYIKVGNYILHFLELSKNSNLLKNSSEMLKHFRLASLMYMYVYTQMI
CPSLLGIRN"
gene complement(<365340..>367892)
/locus_tag="YHR131C"
/db_xref="GeneID:856532"
mRNA complement(<365340..>367892)
/locus_tag="YHR131C"
/product="uncharacterized protein"
/transcript_id="NM_001179261.1"
/db_xref="GeneID:856532"
CDS complement(365340..367892)
/locus_tag="YHR131C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/note="hypothetical protein; GFP-fusion protein localizes
to the cytoplasm; overexpression causes cell cycle delay
or arrest; contains a PH domain and binds
phosphatidylinositols and other lipids in a large-scale
study; YHR131C has a paralog, MPF1, that arose from the
whole genome duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_011999.2"
/db_xref="GeneID:856532"
/db_xref="SGD:S000001173"
/translation="MALPIEGKLSMANNRIERLKSPSSSSTCSMDEVLITSSNNSSSI
CLETMRQLPREGVSGQINIIKETAASSSSHAALFIKQDLYEHIDPLPAYPPSYDLVNP
NKEVRFPIFGDTAPCPKSSLPPLYAPAVYELTLISLKLERLSPYEISSNRSWRNFIIE
INSTQLNFYHIDESLTKHIRNYSSGETKSEKEDRIHSDLVHRSDQSQHLHHRLFTLPT
RSASEFKKADQERISYRVKRDRSRYLTDEALYKSFTLQNARFGIPTDYTKKSFVLRMS
CESEQFLLRFSHIDDMIDWSMYLSIGISVSLDLEVREYPDYRIVPRRRRRRRRRRRRR
RHTHRSESSMGSFSQRFIRSNSRPDLIQRYSTGSSTNNNTTIRERSNTFTAGLLDHYC
TGLSKTPTEALISSAASGESSDNSTLGSTRSLSGCSASRSIASRSLKFKIKNFFRPKN
SSRTEKLHRLRSNSSNLNSVIETEEDDEHHESSGGDHPEPGVPVNTTIKVERPMHRNR
AISMPQRQSLRRAISEEVVPIKFPNSTVGESVHSPSPIEHLSVDGCEIMLQSQNAVMK
EELRSVASNLVANERDEASIRPKPQSSSIYLSGLAPNGESATDLSQSSRSLCLTNRDA
EINDDESATETDEDENDGETDEYAGDDTNDDTDDSNIGYAYGSESDYSCLIEERIRNR
RRASSTLSCFSNIPYGTDDIKWKPAIKEISRRRYLRDSLKCIKPFLDSNDCLGKVIYI
PVSGPTFETSNKIHFSNRQSLQKQKNHFLKGFIVGPTALIELNCKNKNAIVGTTKDAE
DHGEDDGDGDDGEDDDDDDDDDDDDDDDEDDDDDDDDDDDDDDDDDDGQITA"
gene complement(<368502..>369794)
/gene="ECM14"
/locus_tag="YHR132C"
/db_xref="GeneID:856533"
mRNA complement(<368502..>369794)
/gene="ECM14"
/locus_tag="YHR132C"
/product="putative metallocarboxypeptidase"
/transcript_id="NM_001179262.1"
/db_xref="GeneID:856533"
CDS complement(368502..369794)
/gene="ECM14"
/locus_tag="YHR132C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762|PMID:14562095]"
/note="Putative pseudopeptidase; conserved fungal protein
and member of the M14 family of metallocarboxypeptidases
but contains substitutions in active site residues
important for catalysis and lacks activity with standard
substrates; contains a cleavable pro domain; required for
normal cell wall assembly in some strains"
/codon_start=1
/product="putative metallocarboxypeptidase"
/protein_id="NP_012000.1"
/db_xref="GeneID:856533"
/db_xref="SGD:S000001174"
/translation="MLHMNSLWGCFLFVLLAVTGAVQGLQEDYSEYAVYRFTSDNYST
LVRDVIAPLTDDYDVWTRSNNFIDIKLPKEIGEQINDGQVIIDNMNELIQNTLPTSQM
MAREQAVFENDYDFFFNEYRDLDTIYMWLDLLERSFPSLVAVEHLGRTFEGRELKALH
ISGNKPESNPEKKTIVITGGIHAREWISVSTVCWALYQLLNRYGSSKKETKYLDDLDF
LVIPVFNPDGYAYTWSHDRLWRKNRQRTHVPQCLGIDIDHSFGFQWEKAHTHACSEEY
SGETPFEAWEASAWYKYINETKGDYKIYGYIDMHSYSQEILYPYAYSCDALPRDLENL
LELSYGLSKAIRSKSGRNYDVISACKDRGSDIFPGLGAGSALDFMYHHRAHWAFQLKL
RDTGNHGFLLPPENIKPVGKETYAALKYFCDFLLDPEI"
gene <370054..>370449
/gene="IGO2"
/locus_tag="YHR132W-A"
/db_xref="GeneID:856534"
mRNA <370054..>370449
/gene="IGO2"
/locus_tag="YHR132W-A"
/product="phosphatase regulator"
/transcript_id="NM_001184441.1"
/db_xref="GeneID:856534"
CDS 370054..370449
/gene="IGO2"
/locus_tag="YHR132W-A"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0044877
protein-containing complex binding [PMID:28100785]"
/experiment="EXISTENCE:genetic interaction:GO:0034605
cellular response to heat [PMID:23861665]"
/experiment="EXISTENCE:genetic interaction:GO:1900152
negative regulation of nuclear-transcribed mRNA catabolic
process, deadenylation-dependent decay [PMID:20471941]"
/experiment="EXISTENCE:genetic interaction:GO:1901992
positive regulation of mitotic cell cycle phase transition
[PMID:23861665]"
/experiment="EXISTENCE:genetic interaction:GO:1903452
positive regulation of G1 to G0 transition
[PMID:20471941|PMID:23273919]"
/note="Protein required for initiation of the G0 program;
prevents degradation of nutrient-regulated mRNAs via the
5'-3' mRNA decay pathway; phosphorylation (S64) by Rim15p
increases interaction with and inhibition of PP2A-Cdc55
for timely mitotic progression; can also activate
PP2A-Cdc55 complexes and influence Cdc55p localization
along with Igo1p; GFP protein localizes to the cytoplasm
and nucleus; IGO2 has a paralog, IGO1, that arose from the
whole genome duplication"
/codon_start=1
/product="phosphatase regulator"
/protein_id="NP_036194.1"
/db_xref="GeneID:856534"
/db_xref="SGD:S000007496"
/translation="MSEDLSPTSSRVDLSNPHGFTKEGVDLSKLSPQELKLYKMYGKL
PSKKDLLRHKMQDRQYFDSGDYALKKAGVIKSDDVIVNNSSNNLPVTNPSGLRESIIR
RRMSSSSGGDSISRQGSISSGPPPRSPNK"
gene complement(<370722..>371597)
/gene="NSG1"
/locus_tag="YHR133C"
/db_xref="GeneID:856535"
mRNA complement(<370722..>371597)
/gene="NSG1"
/locus_tag="YHR133C"
/product="Nsg1p"
/transcript_id="NM_001179263.1"
/db_xref="GeneID:856535"
CDS complement(370722..371597)
/gene="NSG1"
/locus_tag="YHR133C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762|PMID:16270032]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0034399 nuclear
periphery [PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0071561
nucleus-vacuole junction [PMID:41132095]"
/experiment="EXISTENCE:mutant phenotype:GO:0016126 sterol
biosynthetic process [PMID:16270032]"
/experiment="EXISTENCE:mutant phenotype:GO:0051082
unfolded protein binding [PMID:16270032]"
/experiment="EXISTENCE:physical interaction:GO:0016126
sterol biosynthetic process [PMID:16270032]"
/experiment="EXISTENCE:physical interaction:GO:0051082
unfolded protein binding [PMID:16270032]"
/note="Protein involved in regulation of sterol
biosynthesis; stabilizes Hmg2p, one of two HMG-CoA
isoenzymes that catalyze the rate-limiting step in sterol
biosynthesis; localizes to the nucleus and ER; localizes
to the nucleus-vacuole junction during glucose
restriction; forms foci at the nuclear periphery upon DNA
replication stress; relocalizes to the cytosol in response
to hypoxia; homolog of mammalian INSIG proteins; NSG1 has
a paralog, NSG2, that arose from the whole genome
duplication"
/codon_start=1
/product="Nsg1p"
/protein_id="NP_012001.1"
/db_xref="GeneID:856535"
/db_xref="SGD:S000001175"
/translation="MGKKKSKNQLNTGGVPNGVHNTKKEAALPPLGNKLGSASFTAIN
TLTKPALFSFYDDDITKNEGNVYDKALLSNASQLEMVPPSATARHERSLYAKIINTIA
AFFILFIAGILFPMISECLFDNDQLAKGDIVSFLKHGIEIKNKIVAEPDMVPDWAVFG
TEGVIFGSIVPFIDSFVRYQHQPKTRSSVYKNTLGSFIRCANTLLGLIFGIRKLEWSS
SLQAAGAWSLLNIVLWLFFDGTLTVFFPGLVIGALSAFTCSQCFSQLSLALYFIDFYF
FGFLMFSKLGRYLFN"
gene <371749..>372558
/gene="WSS1"
/locus_tag="YHR134W"
/db_xref="GeneID:856536"
mRNA <371749..>372558
/gene="WSS1"
/locus_tag="YHR134W"
/product="metalloendopeptidase WSS1"
/transcript_id="NM_001179264.1"
/db_xref="GeneID:856536"
CDS 371749..372558
/gene="WSS1"
/locus_tag="YHR134W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26349035]"
/experiment="EXISTENCE:direct assay:GO:0004222
metalloendopeptidase activity [PMID:24998930]"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:18336552]"
/experiment="EXISTENCE:direct assay:GO:0008237
metallopeptidase activity [PMID:26349035]"
/experiment="EXISTENCE:direct assay:GO:0016925 protein
sumoylation [PMID:26349035]"
/experiment="EXISTENCE:direct assay:GO:0019985 translesion
synthesis [PMID:24998930]"
/experiment="EXISTENCE:direct assay:GO:0032183 SUMO
binding [PMID:26349035]"
/experiment="EXISTENCE:direct assay:GO:0061665 SUMO ligase
activity [PMID:26349035]"
/experiment="EXISTENCE:direct assay:GO:1990466 protein
autosumoylation [PMID:26349035]"
/experiment="EXISTENCE:genetic interaction:GO:0016925
protein sumoylation [PMID:11606525]"
/experiment="EXISTENCE:mutant phenotype:GO:0004222
metalloendopeptidase activity [PMID:24998930]"
/experiment="EXISTENCE:mutant phenotype:GO:0019985
translesion synthesis [PMID:24998930]"
/experiment="EXISTENCE:mutant phenotype:GO:0036205 histone
catabolic process [PMID:32130911]"
/experiment="EXISTENCE:mutant phenotype:GO:1990414
replication-born double-strand break repair via sister
chromatid exchange [PMID:23357952]"
/note="SUMO-ligase and SUMO-targeted metalloprotease;
involved in DNA repair; removes DNA-protein crosslinks at
stalled replication forks during replication of damaged
DNA; clears chromatin-bound sumoylated proteins; localizes
to single spot on nuclear periphery of mother cells but
not daughters; exhibits vacuolar localization upon
genotoxic stress; activated by DNA binding; member of
minigluzincins protease family with mammalian
DVC1/Spartan"
/codon_start=1
/product="metalloendopeptidase WSS1"
/protein_id="NP_012002.1"
/db_xref="GeneID:856536"
/db_xref="SGD:S000001176"
/translation="MKTEGIKSPSAKYHDMAGSQRIPHKNPHIQKVAVLQSKPNKEDA
LNLIKEIAHKVSYLMKENHFKVTNLVEFYPRDQRLLGMNVNHGSKIMLRLRCSTDEFQ
FLPMECIMGTMLHELTHNLFGPHDKKFYNKLDELIGRQWVIEQRGLYDTFLGNGQRLG
GRANLRSNRYPMTGISTNTGIVRKRGKGVKLGSLHPEGISSIDRGNSPRELAAFAAER
RYRDDRWCGETKNNKDQIISDNISSSLEVVILDDDDEVLPGDTLIEVIDLT"
gene complement(<372694..>374310)
/gene="YCK1"
/locus_tag="YHR135C"
/gene_synonym="CKI2"
/db_xref="GeneID:856537"
mRNA complement(<372694..>374310)
/gene="YCK1"
/locus_tag="YHR135C"
/gene_synonym="CKI2"
/product="serine/threonine protein kinase YCK1"
/transcript_id="NM_001179265.1"
/db_xref="GeneID:856537"
CDS complement(372694..374310)
/gene="YCK1"
/locus_tag="YHR135C"
/gene_synonym="CKI2"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0004674 protein
serine/threonine kinase activity [PMID:8474447]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836|PMID:8034689|PMID:1627830]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0010255
glucose mediated signaling pathway [PMID:27630263]"
/experiment="EXISTENCE:mutant phenotype:GO:0000902 cell
morphogenesis [PMID:10198058]"
/experiment="EXISTENCE:mutant phenotype:GO:0006897
endocytosis [PMID:10198058]"
/experiment="EXISTENCE:mutant phenotype:GO:0009749
response to glucose [PMID:14755054]"
/note="Palmitoylated plasma membrane-bound casein kinase I
(CK1) isoform; shares redundant functions with Yck2p in
morphogenesis, proper septin assembly, endocytic
trafficking, and glucose sensing; stabilized by Sod1p
binding in the presence of glucose and oxygen, causing
glucose repression of respiratory metabolism; involved in
the phosphorylation and regulation of glucose sensor
Rgt2p; YCK1 has a paralog, YCK2, that arose from the whole
genome duplication"
/codon_start=1
/product="serine/threonine protein kinase YCK1"
/protein_id="NP_012003.1"
/db_xref="GeneID:856537"
/db_xref="SGD:S000001177"
/translation="MSMPIASTTLAVNNLTNINGNANFNVQANKQLHHQAVDSPARSS
MTATTAANSNSNSSRDDSTIVGLHYKIGKKIGEGSFGVLFEGTNMINGVPVAIKFEPR
KTEAPQLRDEYKTYKILNGTPNIPYAYYFGQEGLHNILVIDLLGPSLEDLFDWCGRKF
SVKTVVQVAVQMITLIEDLHAHDLIYRDIKPDNFLIGRPGQPDANNIHLIDFGMAKQY
RDPKTKQHIPYREKKSLSGTARYMSINTHLGREQSRRDDMEALGHVFFYFLRGHLPWQ
GLKAPNNKQKYEKIGEKKRSTNVYDLAQGLPVQFGRYLEIVRSLSFEECPDYEGYRKL
LLSVLDDLGETADGQYDWMKLNDGRGWDLNINKKPNLHGYGHPNPPNEKSRKHRNKQL
QMQQLQMQQLQQQQQQQQYAQKTEADMRNSQYKPKLDPTSYEAYQHQTQQKYLQEQQK
RQQQQKLQEQQLQEQQLQQQQQQQQQLRATGQPPSQPQAQTQSQQFGARYQPQQQPSA
ALRTPEQHPNDDNSSLAASHKGFFQKLGCC"
gene complement(<374654..>375100)
/gene="SPL2"
/locus_tag="YHR136C"
/db_xref="GeneID:856538"
mRNA complement(<374654..>375100)
/gene="SPL2"
/locus_tag="YHR136C"
/product="Spl2p"
/transcript_id="NM_001179266.1"
/db_xref="GeneID:856538"
CDS complement(374654..375100)
/gene="SPL2"
/locus_tag="YHR136C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:11914276]"
/experiment="EXISTENCE:mutant phenotype:GO:0006623 protein
targeting to vacuole [PMID:21143198]"
/note="Protein with similarity to cyclin-dependent kinase
inhibitors; downregulates low-affinity phosphate transport
during phosphate limitation by targeting Pho87p to the
vacuole; upstream region harbors putative hypoxia response
element (HRE) cluster; overproduction suppresses a plc1
null mutation; promoter shows an increase in Snf2p
occupancy after heat shock; GFP-fusion protein localizes
to the cytoplasm"
/codon_start=1
/product="Spl2p"
/protein_id="NP_012004.1"
/db_xref="GeneID:856538"
/db_xref="SGD:S000001178"
/translation="MGTYTPLIYNIYNVHIWVFTESQGQIGQMSPRGKMETAVSQGQH
KQLKDGHQHKGRKLSEEIASLLRLKECRRLNPASYYTPRRTSQSQSLSGSTFKEYNEY
INEKDSSRAQRQNAAAVLSKLAHDFWENDCVIDEDIFEDSSDEEQS"
gene <375709..>377250
/gene="ARO9"
/locus_tag="YHR137W"
/db_xref="GeneID:856539"
mRNA <375709..>377250
/gene="ARO9"
/locus_tag="YHR137W"
/product="aromatic-amino-acid:2-oxoglutarate transaminase"
/transcript_id="NM_001179267.1"
/db_xref="GeneID:856539"
CDS 375709..377250
/gene="ARO9"
/locus_tag="YHR137W"
/EC_number="2.6.1.7"
/EC_number="2.6.1.57"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0006571
L-tyrosine biosynthetic process [PMID:9491082]"
/experiment="EXISTENCE:genetic interaction:GO:0009094
L-phenylalanine biosynthetic process [PMID:9491082]"
/experiment="EXISTENCE:mutant phenotype:GO:0008793
aromatic-amino-acid transaminase activity [PMID:9491082]"
/experiment="EXISTENCE:mutant phenotype:GO:0009072
aromatic amino acid metabolic process [PMID:9491083]"
/note="Aromatic aminotransferase II; involved in tyrosine,
phenylalanine, and methionine metabolism; involved with
Aro8p in production of kynurenic acid to detoxify excess
tryptophan"
/codon_start=1
/product="aromatic-amino-acid:2-oxoglutarate transaminase"
/protein_id="NP_012005.1"
/db_xref="GeneID:856539"
/db_xref="SGD:S000001179"
/translation="MTAGSAPPVDYTSLKKNFQPFLSRRVENRSLKSFWDASDISDDV
IELAGGMPNERFFPIESMDLKISKVPFNDNPKWHNSFTTAHLDLGSPSELPIARSFQY
AETKGLPPLLHFVKDFVSRINRPAFSDETESNWDVILSGGSNDSMFKVFETICDESTT
VMIEEFTFTPAMSNVEATGAKVIPIKMNLTFDRESQGIDVEYLTQLLDNWSTGPYKDL
NKPRVLYTIATGQNPTGMSVPQWKREKIYQLAQRHDFLIVEDDPYGYLYFPSYNPQEP
LENPYHSSDLTTERYLNDFLMKSFLTLDTDARVIRLETFSKIFAPGLRLSFIVANKFL
LQKILDLADITTRAPSGTSQAIVYSTIKAMAESNLSSSLSMKEAMFEGWIRWIMQIAS
KYNHRKNLTLKALYETESYQAGQFTVMEPSAGMFIIIKINWGNFDRPDDLPQQMDILD
KFLLKNGVKVVLGYKMAVCPNYSKQNSDFLRLTIAYARDDDQLIEASKRIGSGIKEFF
DNYKS"
gene complement(<377355..>377699)
/locus_tag="YHR138C"
/db_xref="GeneID:856540"
mRNA complement(<377355..>377699)
/locus_tag="YHR138C"
/product="uncharacterized protein"
/transcript_id="NM_001179268.1"
/db_xref="GeneID:856540"
CDS complement(377355..377699)
/locus_tag="YHR138C"
/experiment="EXISTENCE:mutant phenotype:GO:0004866
endopeptidase inhibitor activity [PMID:9657146]"
/experiment="EXISTENCE:mutant phenotype:GO:0042144 vacuole
fusion, non-autophagic [PMID:9657146]"
/note="hypothetical protein; similar to Pbi2p; double null
mutant lacking Pbi2p and Yhr138cp exhibits highly
fragmented vacuoles; protein abundance increases in
response to DNA replication stress"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_012006.1"
/db_xref="GeneID:856540"
/db_xref="SGD:S000001180"
/translation="MKASYLVLIFISIFSMAQASSLSSYIVTFPKTDNMATDQNSIIE
DVKKYVVDIGGKITHEYSLIKGFTVDLPDSDQILDGLKERLSYIESEYGAKCNLEKDS
EVHALNRDHLVA"
gene complement(<378219..>379199)
/gene="SPS100"
/locus_tag="YHR139C"
/db_xref="GeneID:856541"
mRNA complement(<378219..>379199)
/gene="SPS100"
/locus_tag="YHR139C"
/product="Sps100p"
/transcript_id="NM_001179269.1"
/db_xref="GeneID:856541"
CDS complement(378219..379199)
/gene="SPS100"
/locus_tag="YHR139C"
/experiment="EXISTENCE:mutant phenotype:GO:0030476
ascospore wall assembly [PMID:3280971]"
/note="Protein required for spore wall maturation;
expressed during sporulation; may be a component of the
spore wall; expression also induced in cells treated with
the mycotoxin patulin; SPS100 has a paralog, YGP1, that
arose from the whole genome duplication"
/codon_start=1
/product="Sps100p"
/protein_id="NP_012007.1"
/db_xref="GeneID:856541"
/db_xref="SGD:S000001181"
/translation="MKFTSVLAFFLATLTASATPLYKRQNVTSGGGTVPVIITGGPAV
SGSQSNVTTTTLFNSTSTLNITQLYQIATQVNQTLQSESSSGIIIVTNWRSIETLSFF
CSIVFNTSKTIVITENFLWGVPILSSSDAEGRGTLVAGRDKVVYSGVFPPYTVPVGVL
SGQKNVQWFFDACEPTLIASNSTIRTQYSNFTSAQISSNASSGTNTSSSSSSPLVPII
YEEGYSQSLIQSLSSSIQGLVVVSSGTSHNSTVASWTSVDFPVVYASDGSSGHDGSGI
GFISNTSIPQGAISAGYLSPIQAQTLLSIAIHNQVTSSSELQQIFPVSQQ"
gene 378254..379237
/gene="SUT169"
/locus_tag="YNCH0011W"
/db_xref="GeneID:65052916"
ncRNA 378254..379237
/ncRNA_class="antisense_RNA"
/gene="SUT169"
/locus_tag="YNCH0011W"
/product="SUT169"
/note="Antisense transcript; upregulates
sporulation-specific gene SPS100 upon entry into
starvation or sporulation; functions by changing relative
expression of SPS100 mRNA isoforms from short, unstable
transcript to long, stable species, suggesting novel
mechanism of antisense-dependent gene regulation via mRNA
isoform switching"
/transcript_id="NR_172476.1"
/db_xref="GeneID:65052916"
/db_xref="SGD:S000303809"
gene complement(<380108..>380419)
/locus_tag="YHR139C-A"
/db_xref="GeneID:856542"
mRNA complement(<380108..>380419)
/locus_tag="YHR139C-A"
/product="uncharacterized protein"
/transcript_id="NM_001348830.1"
/db_xref="GeneID:856542"
CDS complement(380108..380419)
/locus_tag="YHR139C-A"
/note="hypothetical protein; conserved across S.
cerevisiae strains"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335742.1"
/db_xref="GeneID:856542"
/db_xref="SGD:S000003533"
/translation="MRSADFSNPRILSNLMSKSEKEKRAIIIFNCRLNKGLYVTSACV
SRSVFRCCKQEPLIEFVSPKMIAFVQIDGRYLTKMLTCDIHVRKEAKEGRGYQQIHKL
S"
gene <380572..>381291
/locus_tag="YHR140W"
/db_xref="GeneID:856543"
mRNA <380572..>381291
/locus_tag="YHR140W"
/product="uncharacterized protein"
/transcript_id="NM_001179270.1"
/db_xref="GeneID:856543"
CDS 380572..381291
/locus_tag="YHR140W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:31945465|PMID:22842922]"
/note="Endoplasmic reticulum protein; may contribute to
maintenance of ER and nuclear morphology; null mutant
shows discontinuity in nuclear envelope, clustering of
nuclear pores, and lacks cortical ER"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_012009.1"
/db_xref="GeneID:856543"
/db_xref="SGD:S000001182"
/translation="MMSCLVPTRFTLTLNTACLLTSTWGFVRATSVVLPPSLSKAGHK
QFLTIISIIATIINNAVNISNYYIQRNNKMNLETKKKSDFISRHVTLPVSLVLESIVA
TVYWPLRLFFVNLIMHGVESTAKTPFPMTVDMAIHLYPILYLLADHYLSGSGTKFKLS
NKHAWLIVTSLAFSYFQYLAFLIDAGQGQAYPYPFLDVNEPYKSIIFVVVATITWAYY
VFYQKFPPKYIKKSAKKGDKN"
gene 381540..381727
/gene="SNR32"
/locus_tag="YNCH0012W"
/db_xref="GeneID:9164901"
ncRNA 381540..381727
/ncRNA_class="snoRNA"
/gene="SNR32"
/locus_tag="YNCH0012W"
/product="SNR32"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:9160748]"
/experiment="EXISTENCE:mutant phenotype:GO:0000454 snoRNA
guided rRNA pseudouridine synthesis [PMID:9160748]"
/experiment="EXISTENCE:mutant phenotype:GO:0030559 rRNA
pseudouridylation guide activity [PMID:9160748]"
/experiment="EXISTENCE:physical interaction:GO:0031429 box
H/ACA snoRNP complex [PMID:15923376]"
/note="H/ACA box small nucleolar RNA (snoRNA); guides
pseudouridylation of large subunit (LSU) rRNA at position
U2191"
/transcript_id="NR_132188.1"
/db_xref="GeneID:9164901"
/db_xref="SGD:S000007297"
gene complement(<381990..>382751)
/gene="RPL42B"
/locus_tag="YHR141C"
/gene_synonym="MAK18"
/db_xref="GeneID:856544"
mRNA complement(join(<381990..382306,382748..>382751))
/gene="RPL42B"
/locus_tag="YHR141C"
/gene_synonym="MAK18"
/product="60S ribosomal protein eL42 RPL42B"
/transcript_id="NM_001179271.1"
/db_xref="GeneID:856544"
CDS complement(join(381990..382306,382748..382751))
/gene="RPL42B"
/locus_tag="YHR141C"
/gene_synonym="MAK18"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:11983894]"
/experiment="EXISTENCE:curator inference:GO:0003735
structural constituent of ribosome [PMID:11983894]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:11983894]"
/note="Ribosomal 60S subunit protein L42B; required for
propagation of the killer toxin-encoding M1
double-stranded RNA satellite of the L-A double-stranded
RNA virus; homologous to mammalian ribosomal protein L36A,
no bacterial homolog; RPL42B has a paralog, RPL42A, that
arose from the whole genome duplication"
/codon_start=1
/product="60S ribosomal protein eL42 RPL42B"
/protein_id="NP_012010.1"
/db_xref="GeneID:856544"
/db_xref="SGD:S000001183"
/translation="MVNVPKTRKTYCKGKTCRKHTQHKVTQYKAGKASLFAQGKRRYD
RKQSGFGGQTKPVFHKKAKTTKKVVLRLECVKCKTRAQLTLKRCKHFELGGEKKQKGQ
ALQF"
gene <383538..>384488
/gene="CHS7"
/locus_tag="YHR142W"
/db_xref="GeneID:856545"
mRNA <383538..>384488
/gene="CHS7"
/locus_tag="YHR142W"
/product="Chs7p"
/transcript_id="NM_001179272.1"
/db_xref="GeneID:856545"
CDS 383538..384488
/gene="CHS7"
/locus_tag="YHR142W"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:22842922|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:10366589]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:29405545|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0006888 endoplasmic
reticulum to Golgi vesicle-mediated transport
[PMID:10366589]"
/experiment="EXISTENCE:mutant phenotype:GO:0006031 chitin
biosynthetic process [PMID:10366589]"
/experiment="EXISTENCE:mutant phenotype:GO:0006457 protein
folding [PMID:15623581]"
/experiment="EXISTENCE:mutant phenotype:GO:0051082
unfolded protein binding [PMID:15623581]"
/note="hypothetical protein; may be involved in chitin
biosynthesis by regulation of Chs3p export from the ER;
relocalizes from bud neck to ER upon DNA replication
stress"
/codon_start=1
/product="Chs7p"
/protein_id="NP_012011.1"
/db_xref="GeneID:856545"
/db_xref="SGD:S000001184"
/translation="MAFSDFAAICSKTPLPLCSVIKSKTHLILSNSTIIHDFDPLNLN
VGVLPRCYARSIDLANTVIFDVGNAFINIGALGVILIILYNIRQKYTAIGRSEYLYFF
QLTLLLIIFTLVVDCGVSPPGSGSYPYFVAIQIGLAGACCWALLIIGFLGFNLWEDGT
TKSMLLVRGTSMLGFIANFLASILTFKAWITDHKVATMNASGMIVVVYIINAIFLFVF
VICQLLVSLLVVRNLWVTGAIFLGLFFFVAGQVLVYAFSTQICEGFKHYLDGLFFGSI
CNVFTLMMVYKTWDMTTDDDLEFGVSVSKDGDVVYDNGFM"
gene <385510..>386487
/gene="DSE2"
/locus_tag="YHR143W"
/db_xref="GeneID:856546"
mRNA <385510..>386487
/gene="DSE2"
/locus_tag="YHR143W"
/product="Dse2p"
/transcript_id="NM_001179274.1"
/db_xref="GeneID:856546"
CDS 385510..386487
/gene="DSE2"
/locus_tag="YHR143W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005576
extracellular region [PMID:11935221]"
/experiment="EXISTENCE:direct assay:GO:0005621 cellular
bud scar [PMID:27229769]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:11309124]"
/experiment="EXISTENCE:mutant phenotype:GO:0000920 septum
digestion after cytokinesis [PMID:11309124]"
/experiment="EXISTENCE:mutant phenotype:GO:0007124
pseudohyphal growth [PMID:11309124]"
/note="Daughter cell-specific secreted protein with
similarity to glucanases; degrades cell wall from the
daughter side causing daughter to separate from mother;
localizes in birth scars; expression is repressed by cAMP"
/codon_start=1
/product="Dse2p"
/protein_id="NP_012012.1"
/db_xref="GeneID:856546"
/db_xref="SGD:S000001186"
/translation="MKFNFSTIFNILFFLFTLIEANSNGETVKLITSDGIVYSYAVYT
KTLAPARVVVKTISYTTTRVYPITLANSVVSSTTEKITEVSTVSASEQVSATQTNSLV
STSTVSTISPTISSGSSTSSSSTYDIESSQSIESSGTSSATAEPSASSGFRLTSSSAF
VSSTAPFSSQLSSSSSSETSSSSFSTSSSSAPLSLTSSSSSSSSFATIITLAPSSSKS
GNSQLTLASSSSTSAVESSQTGSTIARTTSTLVPSSSVDTTSRATTSMPLESSSTQSI
SVSSSDGTCYVFYDDDDYYSTVYLTNPSQSVDAATTITSTNTIYATVTI"
gene <387233..>387445
/gene="RPC10"
/locus_tag="YHR143W-A"
/gene_synonym="RPB12"
/db_xref="GeneID:856547"
mRNA <387233..>387445
/gene="RPC10"
/locus_tag="YHR143W-A"
/gene_synonym="RPB12"
/product="DNA-directed RNA polymerase core subunit RPC10"
/transcript_id="NM_001179273.1"
/db_xref="GeneID:856547"
CDS 387233..387445
/gene="RPC10"
/locus_tag="YHR143W-A"
/gene_synonym="RPB12"
/experiment="EXISTENCE:direct assay:GO:0003899
DNA-directed RNA polymerase activity
[PMID:8288647|PMID:9837969|PMID:3905793]"
/experiment="EXISTENCE:direct assay:GO:0003968
RNA-directed RNA polymerase activity [PMID:18004386]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005665 RNA
polymerase II, core complex [PMID:1748681|PMID:1331084]"
/experiment="EXISTENCE:direct assay:GO:0005666 RNA
polymerase III complex
[PMID:1748681|PMID:3905793|PMID:10611227]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005736 RNA
polymerase I complex [PMID:1748681|PMID:9837969]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:35563734]"
/experiment="EXISTENCE:direct assay:GO:0006360
transcription by RNA polymerase I [PMID:9837969]"
/experiment="EXISTENCE:direct assay:GO:0008270 zinc ion
binding [PMID:1748681]"
/experiment="EXISTENCE:direct assay:GO:0042797 tRNA
transcription by RNA polymerase III [PMID:3905793]"
/experiment="EXISTENCE:genetic interaction:GO:0006383
transcription by RNA polymerase III [PMID:10531351]"
/experiment="EXISTENCE:mutant phenotype:GO:0006360
transcription by RNA polymerase I [PMID:10531351]"
/experiment="EXISTENCE:mutant phenotype:GO:0006366
transcription by RNA polymerase II [PMID:10531351]"
/experiment="EXISTENCE:mutant phenotype:GO:0006383
transcription by RNA polymerase III [PMID:10531351]"
/note="RNA polymerase subunit ABC10-alpha, found in RNA
pol I, II, and III; relocalizes from nucleolus to
cytoplasm upon DNA replication stress; localizes to the
peroxisome in a Pex5p-dependent manner"
/codon_start=1
/product="DNA-directed RNA polymerase core subunit RPC10"
/protein_id="NP_012013.1"
/db_xref="GeneID:856547"
/db_xref="SGD:S000001185"
/translation="MSREGFQIPTNLDAAAAGTSQARTATLKYICAECSSKLSLSRTD
AVRCKDCGHRILLKARTKRLVQFEAR"
gene complement(<387788..>388726)
/gene="DCD1"
/locus_tag="YHR144C"
/db_xref="GeneID:856548"
mRNA complement(<387788..>388726)
/gene="DCD1"
/locus_tag="YHR144C"
/product="deoxycytidine monophosphate deaminase"
/transcript_id="NM_001179275.1"
/db_xref="GeneID:856548"
CDS complement(387788..388726)
/gene="DCD1"
/locus_tag="YHR144C"
/EC_number="3.5.4.12"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0004132 dCMP
deaminase activity [PMID:3023902]"
/experiment="EXISTENCE:mutant phenotype:GO:0006226 dUMP
biosynthetic process [PMID:6373725]"
/experiment="EXISTENCE:mutant phenotype:GO:0006231 dTMP
biosynthetic process [PMID:6373725]"
/note="Deoxycytidine monophosphate (dCMP) deaminase;
involved in dUMP and dTMP biosynthesis; expression is NOT
cell cycle regulated"
/codon_start=1
/product="deoxycytidine monophosphate deaminase"
/protein_id="NP_012014.1"
/db_xref="GeneID:856548"
/db_xref="SGD:S000001187"
/translation="MLIGVSGTKFCGCEDVINMLVDHFHFELLNHLDNPEEILDYATK
NYTKNSVIFLEKLSLLEKLEKRPFFVHLSIDAPVTTRVALYRKTTQAESLSLEQIIQA
IDQHDFQPEGIKLREKSHLRFKIVNEDRRGRRQSLINNITTQLKILDDKEKQMAPLMR
PSWDSYFMKLATLAASRSNCMKRRVGCVIVRECRVIATGYNGTPRHLTNCFNGGCPRC
NDGDSRNLHTCLCLHAEENALLEAGRDRVGQNATLYCDTCPCLTCSVKIVQTGISEVV
YSQSYRMDEESFKVLKNAGITVRQFSFTEEPRIVMI"
gene complement(388893..388995)
/gene="SUF8"
/locus_tag="YNCH0013C"
/db_xref="GeneID:856549"
tRNA complement(join(388893..388928,388960..388995))
/gene="SUF8"
/locus_tag="YNCH0013C"
/product="tRNA-Pro"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE
analysis; target of K. lactis zymocin; can mutate to
suppress +1 frameshift mutations in proline codons"
/db_xref="GeneID:856549"
/db_xref="SGD:S000006682"
repeat_region complement(389177..389509)
/note="Ty2 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006997"
repeat_region complement(389613..389845)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006998"
gene <390300..>391697
/gene="CRP1"
/locus_tag="YHR146W"
/db_xref="GeneID:856551"
mRNA <390300..>391697
/gene="CRP1"
/locus_tag="YHR146W"
/product="Crp1p"
/transcript_id="NM_001179277.1"
/db_xref="GeneID:856551"
CDS 390300..391697
/gene="CRP1"
/locus_tag="YHR146W"
/experiment="EXISTENCE:curator inference:GO:0005634
nucleus [PMID:12419258]"
/experiment="EXISTENCE:direct assay:GO:0003677 DNA binding
[PMID:12419258]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:38832010]"
/note="Protein that binds to cruciform DNA structures;
CRP1 has a paralog, MDG1, that arose from the whole genome
duplication"
/codon_start=1
/product="Crp1p"
/protein_id="NP_012016.1"
/db_xref="GeneID:856551"
/db_xref="SGD:S000001189"
/translation="MSSELMFNYTFSWPAGPKDVILTGTFDDWRGTLPLVKTAKGNFE
ITMPVKLANKDDTFQFKFIVDGVWCVSDSYKKEHVSEGIENNFLQITDLVETQEVAGA
SRIPEAGGLLCGKPPRSAGPPSTSNRKKNKRNNKKRRSKLKKKSTKNNKKSNESLDDN
EEEDGVTGTTTEDVTGTSREETPLAEPTNVSKEAPGNFHILPIDQSADTTQSNGIIGG
PGPVLVPNPGEIKEFTEIRDVDARELNERLNKKEEVPEPVAGPIVESSVTEKSPALPQ
ADDPIVETKEVAHNVQELTPQVEAVTPLINEPEPLPTPEAQISIPESSKVEPVEGSLQ
SKLVEKRESTEGVLDGSKKVENKAKKDEEVFTLDPIVNKAPKLPLTDEQTAEGRKSPA
VSEEKEKKKKQEKGSKEVKRSETSKEKKPSAKEVKKQTVKAPKKQTASPLSSSTEEPK
KKKTGFFGKLKKLFK"
rep_origin 392180..392395
/note="ARS818; Autonomously Replicating Sequence"
/db_xref="SGD:S000118360"
gene complement(<392639..>393283)
/gene="MRPL6"
/locus_tag="YHR147C"
/gene_synonym="uL6m"
/db_xref="GeneID:856552"
mRNA complement(<392639..>393283)
/gene="MRPL6"
/locus_tag="YHR147C"
/gene_synonym="uL6m"
/product="mitochondrial 54S ribosomal protein uL6m MRPL6"
/transcript_id="NM_001179278.1"
/db_xref="GeneID:856552"
CDS complement(392639..393283)
/gene="MRPL6"
/locus_tag="YHR147C"
/gene_synonym="uL6m"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:9151978|PMID:12392552]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:12392552|PMID:9151978]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005762
mitochondrial large ribosomal subunit
[PMID:9151978|PMID:12392552]"
/note="Mitochondrial ribosomal protein of the large
subunit"
/codon_start=1
/product="mitochondrial 54S ribosomal protein uL6m MRPL6"
/protein_id="NP_012017.1"
/db_xref="GeneID:856552"
/db_xref="SGD:S000001190"
/translation="MSFIQRRLLSQTLFLRSQVGSLPLYISPEVQVSINALSMPRIIR
KGRTSMNISQNITVKGPKGELSVEVPDFLHLDKDEKHGKINVTVQNSEDKHQRSMWGT
VRSLINNHIIGVTEGHLAVLRFVGTGYRAQLENDGKFVNVKVGASIKQGLDVPEGIVV
KTPAPTSLIIEGCNKQQVLLFAAKLRKFHPPEPYKGKGIYVNDETIKLKDKKIK"
gene <393534..>394085
/gene="IMP3"
/locus_tag="YHR148W"
/db_xref="GeneID:856553"
mRNA <393534..>394085
/gene="IMP3"
/locus_tag="YHR148W"
/product="snoRNA-binding rRNA-processing protein IMP3"
/transcript_id="NM_001179279.1"
/db_xref="GeneID:856553"
CDS 393534..394085
/gene="IMP3"
/locus_tag="YHR148W"
/experiment="EXISTENCE:direct assay:GO:0006364 rRNA
processing [PMID:23980203]"
/experiment="EXISTENCE:direct assay:GO:0030515 snoRNA
binding [PMID:15489263|PMID:23980203]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/experiment="EXISTENCE:direct assay:GO:0032040
small-subunit processome [PMID:12068309]"
/experiment="EXISTENCE:direct assay:GO:0034457 Mpp10
complex [PMID:17515605]"
/experiment="EXISTENCE:direct assay:GO:0042274 ribosomal
small subunit biogenesis [PMID:23980203]"
/experiment="EXISTENCE:direct assay:GO:0140691 RNA folding
chaperone [PMID:23980203|PMID:15489263]"
/experiment="EXISTENCE:mutant phenotype:GO:0032040
small-subunit processome [PMID:12242301]"
/note="Subunit of the MPP10 chaperone complex and the
small subunit (SSU) processome; contains U3 snoRNA binding
and RNA chaperone activities; SSU processome is required
for pre-18S rRNA processing; essential protein that
interacts with Mpp10p and mediates interactions of Imp4p
and Mpp10p with U3 snoRNA"
/codon_start=1
/product="snoRNA-binding rRNA-processing protein IMP3"
/protein_id="NP_012018.1"
/db_xref="GeneID:856553"
/db_xref="SGD:S000001191"
/translation="MVRKLKHHEQKLLKKVDFLEWKQDQGHRDTQVMRTYHIQNREDY
HKYNRICGDIRRLANKLSLLPPTDPFRRKHEQLLLDKLYAMGVLTTKSKISDLENKVT
VSAICRRRLPVIMHRLKMAETIQDAVKFIEQGHVRVGPNLINDPAYLVTRNMEDYVTW
VDNSKIKKTLLRYRNQIDDFDFS"
gene complement(<394455..>396659)
/gene="SKG6"
/locus_tag="YHR149C"
/db_xref="GeneID:856554"
mRNA complement(<394455..>396659)
/gene="SKG6"
/locus_tag="YHR149C"
/product="Skg6p"
/transcript_id="NM_001179280.1"
/db_xref="GeneID:856554"
CDS complement(394455..396659)
/gene="SKG6"
/locus_tag="YHR149C"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:16314687]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:16314687|PMID:11489916]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:11489916]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:16314687]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:16314687]"
/experiment="EXISTENCE:genetic interaction:GO:1902413
negative regulation of mitotic cytokinesis
[PMID:16816427]"
/experiment="EXISTENCE:mutant phenotype:GO:1902413
negative regulation of mitotic cytokinesis
[PMID:16816427]"
/note="Integral membrane protein; localizes primarily to
growing sites such as the bud tip or the cell periphery;
potential Cdc28p substrate; Skg6p interacts with Zds1p and
Zds2p; SKG6 has a paralog, TOS2, that arose from the whole
genome duplication"
/codon_start=1
/product="Skg6p"
/protein_id="NP_012019.1"
/db_xref="GeneID:856554"
/db_xref="SGD:S000001192"
/translation="MYHTHMHESLISVTSTVSVSDASYAYARLTRRDDSDSSSSSASS
TKNSKSAECTGSKQQCQLPTDSSHSTSVTVGVAVAVPVGVIIIVLAVILCIVYRRSKK
EAEEDNDPDFEGDSEFLPTMKDYSPGINHLYSSDSQQDFMEKTLQQPPSDPFVGSMHS
SKYNVRSATPPAIGRSWYVDPFQLPQESNDSNSLRDFAMRVQEDGLGGYKVAAESRNA
SQTSLHPDNFSNCTPIRASSRFQESESFRSHGSPIHNNQLSRGSATEGANKQFTFPNE
DNDSSSVSEEAEVLNESNESASNDAFEFELDNSSEKTHERNLRFGKDDDNYELQDIRE
AEHMNDRSSSKSQDDDYYVSLLSPNEEEDIKRMKSIYQVYLDRAKTMKKEEDKADNAN
DISQEENRVDNIVQNPLPSIKINNNDNIDNNEVPEAKHLVKEALPLNNTNLAEYGPEM
AQSQKQYPVQDTLTVNDTEAAPSNRIASSIYSEAIQPLNYQDQYQQQEQSPVYNGHTQ
YPGNGYSGNPQQQGYTAQFVQNPQWYGVPTPQQQQHNHPQTLETIGELPTPAYLAQSA
SSHSLTSFKRPNKQQLLQLQTARLNGTALNPVDHPEMFYSPTNDAYYAPQQQGQYMKF
NENGAVPSPYQLRQSVVMTNPSDLTAKPSYKPAGSFRSVSATNSRNNSLTTQNNIYLQ
QQQQQLYNSRVSGILEETDVVQPPSVGGILPHSGSQDDLRKQLGSSHNYTVN"
gene <397251..>398990
/gene="PEX28"
/locus_tag="YHR150W"
/db_xref="GeneID:856555"
mRNA <397251..>398990
/gene="PEX28"
/locus_tag="YHR150W"
/product="Pex28p"
/transcript_id="NM_001179281.1"
/db_xref="GeneID:856555"
CDS 397251..398990
/gene="PEX28"
/locus_tag="YHR150W"
/experiment="EXISTENCE:direct assay:GO:0005778 peroxisomal
membrane [PMID:12707309]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0007031 peroxisome
organization [PMID:12707309]"
/experiment="EXISTENCE:mutant phenotype:GO:0007031
peroxisome organization [PMID:12707309]"
/note="Peroxisomal integral membrane peroxin; involved in
the regulation of peroxisomal size, number and
distribution; genetic interactions suggest that Pex28p and
Pex29p act at steps upstream of those mediated by Pex30p,
Pex31p, and Pex32p"
/codon_start=1
/product="Pex28p"
/protein_id="NP_012020.1"
/db_xref="GeneID:856555"
/db_xref="SGD:S000001193"
/translation="MSETSSSRRSASKDAVKSYFAGKYNKVLDSILEAEAAISKSPTV
AEDLSGSSSSGNSEMSHPSLTASSATSQGISKKELLQQIAGSLFSTSIERLKTAHSSE
VSSTPEYSVNDSYGEQECRECDGVFKCSAHFEGAPEYYDDETESGPALEPMTSNSEKD
PFIDVFLDKLISRLVPEKLPEREHFSSKTTIEHDLDTGRVPVFSATTLGSNFKKLSKK
MGSIFELQDSIVRLLTWRNPTGTVTSLILFTLICFNPMYLVILPIFRFVYGIVVPGYV
RKHPLQRSIYPLKRNHGSSLLYDVCYEGKNEYSYGQQFFSKSFMDTLESRNQEIDEIS
ELDKRTENTGELKQGMKVLINLRDMQNMTSGTLHVIEAINSFLRKSSSFQNEECSTKR
FFTGFLLIVFLKILSPFVNWSYVCSIFAWCLLIYMHPRAHPKIISFFKTGTMGKEYKN
LKKREHQALNMIFDEQPETKFIEIFEIYKKALLPNDWKFFRYSNRIFDPQDPYRRAQQ
FPPGVDSLADVIPPTGWSFDPNFEWKIDNDVDRWVVERGLNLPITGEFLFDPMFKRRR
LIHRVIKNATPVA"
gene complement(<399268..>400848)
/gene="MTC6"
/locus_tag="YHR151C"
/db_xref="GeneID:856556"
mRNA complement(<399268..>400848)
/gene="MTC6"
/locus_tag="YHR151C"
/product="Mtc6p"
/transcript_id="NM_001179282.1"
/db_xref="GeneID:856556"
CDS complement(399268..400848)
/gene="MTC6"
/locus_tag="YHR151C"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:38621657]"
/experiment="EXISTENCE:mutant phenotype:GO:0071464
cellular response to hydrostatic pressure [PMID:38621657]"
/note="ER-resident glycoprotein involved in high-pressure
tolerance; proposed role promoting proper folding of
nutrient permeases, such as Tat2p, in the ER during
hydrostatic pressure stress and maintaining permease
conformational stability; contains two transmembrane
domains, a C-terminal cytoplasmic domain and a luminal
region with 12 N-linked glycans; null is synthetically
sick with cdc13-1; localizes to the ER membrane"
/codon_start=1
/product="Mtc6p"
/protein_id="NP_012021.1"
/db_xref="GeneID:856556"
/db_xref="SGD:S000001194"
/translation="MWILIYLFIIWSSLRTWVTAVDSTTTVGDDLNETVSASVWPTMS
PQMTVAFRSQRDVMGNLTIDQLPYVGLNLRRVLLNNETSMVNEGNNTRLLTLFKSMLS
SEANAFVLDLEQYNNDLRVVDTTLLFSDVLTALQSFIFSTQNNLYANIIVLLLNISAP
ELDSTEYRHQNQTLNTTYILDKNLGNSFIYKPTDLQSDRAKNNTWNIYGKSSIDGWPT
LGSVLYEQKKRLVIGELTDFFNETTAPYIFPHDVFHYEQGNSTLDCPSTVEGLTDLSS
IHWRFLDSLFNSVDIKEYISCGLSPIISNSAYVNNVTQLADIIHEGSVWSWDSDQPSV
TQSTSKSGSSSGTLEAYNCVLLYYFANNETVTWRVGNCYNSNIGLCRYENMAFRWLVR
SNKATYFDFDSYQGSKCPDQYSFNIPRSPLEQRSFIAYMRNSSFSDTQIWIDLNSISV
SNCWVSGGPYASCPYEKVISRRNFVTMMVPASVCSFALLCIVVYLSVLRVPIYDNRKN
WRRVINKISKSELEGVPS"
gene <401434..>401955
/gene="SPO12"
/locus_tag="YHR152W"
/gene_synonym="SDB21"
/db_xref="GeneID:856557"
mRNA <401434..>401955
/gene="SPO12"
/locus_tag="YHR152W"
/gene_synonym="SDB21"
/product="Spo12p"
/transcript_id="NM_001179283.1"
/db_xref="GeneID:856557"
CDS 401434..401955
/gene="SPO12"
/locus_tag="YHR152W"
/gene_synonym="SDB21"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10564265|PMID:11729145]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:11729145]"
/experiment="EXISTENCE:genetic interaction:GO:0031536
positive regulation of exit from mitosis [PMID:11832211]"
/experiment="EXISTENCE:genetic interaction:GO:1904750
negative regulation of protein localization to nucleolus
[PMID:14551257]"
/experiment="EXISTENCE:mutant phenotype:GO:0007127 meiosis
I [PMID:7021311]"
/experiment="EXISTENCE:mutant phenotype:GO:0031536
positive regulation of exit from mitosis
[PMID:27418100|PMID:11832211]"
/experiment="EXISTENCE:mutant phenotype:GO:1904750
negative regulation of protein localization to nucleolus
[PMID:14551257]"
/note="Nucleolar hypothetical protein; positive regulator
of mitotic exit; involved in regulating release of Cdc14p
from the nucleolus in early anaphase, may play similar
role in meiosis; SPO12 has a paralog, BNS1, that arose
from the whole genome duplication"
/codon_start=1
/product="Spo12p"
/protein_id="NP_012022.1"
/db_xref="GeneID:856557"
/db_xref="SGD:S000001195"
/translation="MSNKASDQSARTASILKTDITRENTITRSSSSNNDNYHHHNNIN
NYNESAKTGEDANKENIPNLEEEIAAFRIFRKKSTSNLKSSHTTSNLVKKTMFKRDLL
KQDPKRKLQLQQRFASPTDRLVSPCSLKLNEHKVKMFGKKKKVNPMKLNFKGNLAADS
EDVEIDEDEEYFY"
gene complement(<402086..>402682)
/gene="SPO16"
/locus_tag="YHR153C"
/db_xref="GeneID:856558"
mRNA complement(<402086..>402682)
/gene="SPO16"
/locus_tag="YHR153C"
/product="Spo16p"
/transcript_id="NM_001179284.1"
/db_xref="GeneID:856558"
CDS complement(402086..402682)
/gene="SPO16"
/locus_tag="YHR153C"
/experiment="EXISTENCE:direct assay:GO:0000217 DNA
secondary structure binding [PMID:30566683]"
/experiment="EXISTENCE:direct assay:GO:0000228 nuclear
chromosome [PMID:30566683]"
/experiment="EXISTENCE:mutant phenotype:GO:0007130
synaptonemal complex assembly [PMID:18297071]"
/experiment="EXISTENCE:mutant phenotype:GO:0010520
regulation of reciprocal meiotic recombination
[PMID:18297071]"
/experiment="EXISTENCE:mutant phenotype:GO:0030437
ascospore formation [PMID:2188099]"
/experiment="EXISTENCE:mutant phenotype:GO:0033235
positive regulation of protein sumoylation
[PMID:23326245]"
/note="Meiosis-specific protein involved in synaptonemal
complex assembly; implicated in regulation of crossover
formation; required for sporulation"
/codon_start=1
/product="Spo16p"
/protein_id="NP_012023.1"
/db_xref="GeneID:856558"
/db_xref="SGD:S000001196"
/translation="MSEFFWDVQKIQEISNVEEHSVVKCVTVNTSRLISQLNEELQDE
ESGVNFIVTQLQLLINNVYEKIQKSPGVPAHRSLMINLNFTRLKFSIAYWDILLERSL
DLINGPSKTGARYFITEVTPVDRSRYVENNQYFLAFKANQRLTRNSVDMDEFIDFEIL
IKQIIFDLFKKNGIPDQDFEAILSRFHNLESLVVAFNE"
gene <402966..>406178
/gene="RTT107"
/locus_tag="YHR154W"
/gene_synonym="ESC4"
/db_xref="GeneID:856559"
mRNA <402966..>406178
/gene="RTT107"
/locus_tag="YHR154W"
/gene_synonym="ESC4"
/product="Rtt107p"
/transcript_id="NM_001179285.1"
/db_xref="GeneID:856559"
CDS 402966..406178
/gene="RTT107"
/locus_tag="YHR154W"
/gene_synonym="ESC4"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:17978089]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0035361 Cul8-RING
ubiquitin ligase complex [PMID:20139071]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0090734 site of DNA
damage [PMID:38473708]"
/experiment="EXISTENCE:mutant phenotype:GO:0006302
double-strand break repair [PMID:14988729]"
/experiment="EXISTENCE:mutant phenotype:GO:0010526
transposable element silencing [PMID:11779788]"
/experiment="EXISTENCE:mutant phenotype:GO:1903775
regulation of DNA double-strand break processing
[PMID:26490958]"
/experiment="EXISTENCE:mutant phenotype:GO:1990683 DNA
double-strand break attachment to nuclear envelope
[PMID:27056668]"
/experiment="EXISTENCE:mutant phenotype:GO:2000001
regulation of DNA damage checkpoint [PMID:26490958]"
/note="BRCA1 C-terminal domain protein needed for recovery
from DNA damage; implicated in Mms22-dependent DNA repair
during S phase; involved in recruiting SMC5/6 complex to
double-strand breaks; DNA damage induces phosphorylation
by Mec1p at one or more SQ/TQ motifs; interacts with
Mms22p and Slx4p; has four BRCT domains; has a role in
regulation of Ty1 transposition; relative distribution to
nuclear foci increases upon DNA replication stress"
/codon_start=1
/product="Rtt107p"
/protein_id="NP_012024.1"
/db_xref="GeneID:856559"
/db_xref="SGD:S000001197"
/translation="MSTSLLFEQLNFLILVAAEAELPIAHSTRKLLMDNSCNNCQIYE
LYNENLKDVKTDKDWFMNKFGPQTVHFVISNTINFPFYKIVYFDLLIPVVSHTWVQDS
VKTKRHLRTNMYSPNPFHLLRDCQVYISKSSFNKCEYILYSDLLHLLGGTLVNYISNR
TTHVIVQSPQDPIIATVSKLTFGSFSSSSTNKHTEKPLREWKFVYPIWILYHFKMAKP
LKGELATLCELDMQDTSEEQLFAKWEEVIGDKQTSSSQLTLHPNKTLFKNHHFAISPD
LNFFTPLYWFLKGFIEDLDGKVTPLSFSDDLKSVYQAFPDIDCYIGHSANSPILEKTK
SIKPEIHVGNVSWLFYMFALQKFTPVSQCKLIHQPFHAKLFTSKELTVAYTNYFGSQR
FYIQRLVEILGGLSTPELTRKNTHLITKSTIGKKFKVAKKWSLDPQNAIIVTNHMWLE
QCYMNNSKLNPKDSRFQNFKLDDNMGWNIGQIGMDHSSLPTPKNLSMVTYDTQSISEK
PPPTNDQMDQINDNTNVLSKKDGTPISSFENSIDEKIDKLQKISGEVAVTHSGDLERS
FVSRPSRASFPVVDSKKSNLQKKDSNSDISMETEVFCEGHEKREEKEFTKPITEYDAP
KKQEIREQSRKKNDIDYKKEEEETELQVQLGQRTKREIKTSKKNEKEKETNECHIEVD
QMTNEKQGEESTGKLISTEDVTSKKDTDKFSHLFEGLSDNDDHINDEKPAVNSKYTTP
KTSQNITSGVDTPTTAQTQVFMPSSGNSRLAKTQAAKRLHTDIESLNEFQKNFKRKRI
DSEEISLSQDVERSNNNKELATKAEKILARFNELPNYDLKAVCTGCFHDGFNEVDIEI
LNQLGIKIFDNIKETDKLNCIFAPKILRTEKFLKSLSFEPLKFALKPEFIIDLLKQIH
SKKDKLSQININLFDYEINGINESIISKTKLPTKVFERANIRCINLVNDIPGGVDTIG
SVLKAHGIEKINVLRSKKCTFEDIIPNDVSKQENGGIFKYVLIVTKASQVKKFTKLIN
DRDKNETILIVEWNWCVESIFHLNVDFTSKKNVLYQKKNN"
gene <407103..>410789
/gene="LAM1"
/locus_tag="YHR155W"
/gene_synonym="YSP1"
/db_xref="GeneID:856560"
mRNA <407103..>410789
/gene="LAM1"
/locus_tag="YHR155W"
/gene_synonym="YSP1"
/product="Lam1p"
/transcript_id="NM_001179286.1"
/db_xref="GeneID:856560"
CDS 407103..410789
/gene="LAM1"
/locus_tag="YHR155W"
/gene_synonym="YSP1"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:15657396|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0032541 cortical
endoplasmic reticulum [PMID:26001273]"
/experiment="EXISTENCE:mutant phenotype:GO:0032366
intracellular sterol transport [PMID:26001273]"
/note="Putative sterol transfer protein; localizes to
puncta in the cortical ER; probable role in retrograde
transport of sterols from the plasma membrane to the ER;
one of six StART-like domain-containing proteins in yeast
that may be involved in sterol transfer between
intracellular membranes; conserved across eukaryotes;
contains GRAM, StART-like (VASt) and two PH-like domains"
/codon_start=1
/product="Lam1p"
/protein_id="NP_012025.1"
/db_xref="GeneID:856560"
/db_xref="SGD:S000001198"
/translation="MHEHKAELRLITVALNEASTDSPSFRASVNYFHTRMESLSSWMH
STVDYVENTYKPSFQDFQRIKETLFSQLLPSPILLSNGFVSNQPYTPLLVRDFTRDVS
DLSNTVMKIILGDENSQYTAALSALSSDAINPYFNKRKTFEYYQRKYDSFLTDFLAAT
NDGNTLIPQNLQNETFKLVDIKHKYIEASLDLTEAISLMKVNLDKFLIETIDIVRKNN
VITTKDTKDVIDITPELTETLKDWTDWIESNLQTLQALSSKLSEAKYAILKLSLARMK
PSRLIQDYDLKSIQNLKFNLPKSISNGNNSEEKGLSGWLYMKTTVGHDPKRVVWVRRW
CFLQNNVFGVFSLSPSKTYVEETDKFGILWITVEYLPKEPRNFCFKLRIQNPNCKTEE
ENTYIDIILQAESIDELKSWINTLTSHKRIALSIKEENDPRYQLARKKIEPQFFEFAS
SSSTSTDKLLTSFSSKTLTLVEELKKNYMSEDDIYSIIDNKAYHLRVISTPIATQLTH
LALFSTFLSVSNYYPCATQANTWGTANWNDLSYLVNPLKGSSVHKPATVSNSSRFSVS
YPDYYPYSLKVDDIQFRSIFFSVNHDFLQVPKELVLLRYSSVWCPNNKQKFASMAFVT
LNHIYVYLNISGFSYLRRIDLLDIDSIEYDKSPKHVSSRMLHMQRGDGLRFNMSVFFT
DRRAVASKLQFLIENKAMHIPKGEKEVLEIFQELDEEIENEKKIIKDNLSESEHYSKD
YDYLLKSTYDHHFENTNETPMELMSRKLRLEREAWCYFQDNFKVGSKTLFHVLFGDKS
QVFPSSLFLCKKGSNLNNNSYWERIRRAKEDASCQFELCRKLQFQLNRTSNFIKDLLW
LKDDNDNFKLVLQQRVTKIKQGYYFEVEEGPIIVKFPLCHPLLLRVRFIIAECITSQG
ESLKKCDLAILYDFNYVESIDKLNTKVEKLWLFERIHLNWALRYCKLEHSEINRKTRE
YLKKFNDREKMSDVIKLCGFLGVLPKERIENDEKAGDFMQPVYINYDFLSLSKIFTKL
TVFYLSSVIIKTMKVLLAMVMVIFKCFSKVNKTLYYCLLISAVTNLFFVGKSIHSYFS
VKSAETLFQNYANGDQRGLQIMHRSLTVPDLNLLTRKMMDNDQENPVFKRFDEDKNAY
QYKGTRQEIAIKRNQVLTELKILQNTEKELVQGSYRKFIITERDKCITTQNEIFDLWI
NDTKLQDYCMACFAEYNRLSAIPV"
gene 411228..411317
/gene="SNR71"
/locus_tag="YNCH0014W"
/db_xref="GeneID:9164902"
ncRNA 411228..411317
/ncRNA_class="snoRNA"
/gene="SNR71"
/locus_tag="YNCH0014W"
/product="SNR71"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:10024243]"
/experiment="EXISTENCE:curator inference:GO:0031428 box
C/D methylation guide snoRNP complex [PMID:10024243]"
/experiment="EXISTENCE:mutant phenotype:GO:0030562 rRNA
2'-O-ribose methylation guide activity [PMID:10024243]"
/experiment="EXISTENCE:mutant phenotype:GO:0031167 rRNA
methylation [PMID:10024243]"
/note="C/D box small nucleolar RNA (snoRNA); guides
2'-O-methylation of large subunit (LSU) rRNA at position
A2946"
/transcript_id="NR_132189.1"
/db_xref="GeneID:9164902"
/db_xref="SGD:S000006462"
gene complement(<411384..>412406)
/gene="LIN1"
/locus_tag="YHR156C"
/gene_synonym="SNU40"
/db_xref="GeneID:856561"
mRNA complement(<411384..>412406)
/gene="LIN1"
/locus_tag="YHR156C"
/gene_synonym="SNU40"
/product="U5 snRNP complex subunit LIN1"
/transcript_id="NM_001179287.1"
/db_xref="GeneID:856561"
CDS complement(411384..412406)
/gene="LIN1"
/locus_tag="YHR156C"
/gene_synonym="SNU40"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:12402242]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12402242]"
/experiment="EXISTENCE:direct assay:GO:0005682 U5 snRNP
[PMID:11720284]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:12402242]"
/note="Non-essential component of U5 snRNP; nuclear
protein; physically interacts with Irr1p of cohesin
complex; may link together proteins involved in chromosome
segregation, mRNA splicing and DNA replication"
/codon_start=1
/product="U5 snRNP complex subunit LIN1"
/protein_id="NP_012026.1"
/db_xref="GeneID:856561"
/db_xref="SGD:S000001199"
/translation="MKYTQYPNSSKLKRNSDRREHDEKLSDELHNQSTIYEDEELSRA
EYDSDSDSSVEDSTDNENSGKEMDEKSYEKNEDHVEDHRKRKKSKIQLLDIAEFKKEN
LADLDYQIGNSESKVEKGVNIEPFNIDDEIKHGVFDKDGNYIKTENATENDQQDNEEW
MNDVINTEEVNRLEKEQSVKTQNSRHYMVHEALNLLKFFLVDENETVLESLGRLNKLR
KIAISKKNKSLKYVIHGIELLSDLINILEKKGFSEVYEYNRLKVQDAIEEEIFDDSSR
IVNHKTKLWGFKWLNKLDEYHGLYTNYEMSYWQKSYFKNSVIVKFHSEPDRDENWIHV
SCLSFM"
gene <412907..>413455
/gene="REC104"
/locus_tag="YHR157W"
/db_xref="GeneID:856562"
mRNA <412907..>413455
/gene="REC104"
/locus_tag="YHR157W"
/product="Rec104p"
/transcript_id="NM_001179288.1"
/db_xref="GeneID:856562"
CDS 412907..413455
/gene="REC104"
/locus_tag="YHR157W"
/experiment="EXISTENCE:direct assay:GO:0000794 condensed
nuclear chromosome [PMID:15044957]"
/experiment="EXISTENCE:mutant phenotype:GO:0007131
reciprocal meiotic recombination [PMID:9177999]"
/note="Protein involved in early stages of meiotic
recombination; required for meiotic crossing over; forms a
complex with Rec102p and Spo11p necessary during the
initiation of recombination"
/codon_start=1
/product="Rec104p"
/protein_id="NP_012027.1"
/db_xref="GeneID:856562"
/db_xref="SGD:S000001200"
/translation="MSIEEEDTNKITCTQDFLHQYFVTERVSIQFGLNNKTVKRINKD
EFDKAVNCIMSWTNYPKPGLKRTASTYLLSNSFKKSATVSLPFILGDPVCMPKRVESN
NNDTCLLYSDTLYDDPLIQRNDQAGDEIEDEFSFTLLRSEVNEIRPISSSSTAQILQS
DYSALMYERQASNGSIFQFSSP"
gene complement(<413685..>417179)
/gene="KEL1"
/locus_tag="YHR158C"
/db_xref="GeneID:856563"
mRNA complement(<413685..>417179)
/gene="KEL1"
/locus_tag="YHR158C"
/product="Kel1p"
/transcript_id="NM_001179289.1"
/db_xref="GeneID:856563"
CDS complement(413685..417179)
/gene="KEL1"
/locus_tag="YHR158C"
/experiment="EXISTENCE:direct assay:GO:0005515 protein
binding [PMID:34965431]"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:9786949]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:9786949]"
/experiment="EXISTENCE:direct assay:GO:0005938 cell cortex
[PMID:23673619]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807|PMID:9786949]"
/experiment="EXISTENCE:direct assay:GO:1990615
Kelch-containing formin regulatory complex
[PMID:24828508]"
/experiment="EXISTENCE:genetic interaction:GO:0032465
regulation of cytokinesis [PMID:24828508]"
/experiment="EXISTENCE:genetic interaction:GO:0090337
regulation of formin-nucleated actin cable assembly
[PMID:24828508]"
/experiment="EXISTENCE:mutant phenotype:GO:0000755
cytogamy [PMID:9786949]"
/experiment="EXISTENCE:mutant phenotype:GO:0001100
negative regulation of exit from mitosis [PMID:12234925]"
/experiment="EXISTENCE:mutant phenotype:GO:0005737
cytoplasm [PMID:34965431]"
/experiment="EXISTENCE:mutant phenotype:GO:0008360
regulation of cell shape [PMID:9786949]"
/experiment="EXISTENCE:mutant phenotype:GO:0060627
regulation of vesicle-mediated transport [PMID:24828508]"
/experiment="EXISTENCE:mutant phenotype:GO:0090029
negative regulation of pheromone-dependent signal
transduction involved in conjugation with cellular fusion
[PMID:34965431]"
/note="Protein required for proper cell fusion and cell
morphology; acts as phosphorylation-regulated noise
suppressor of pheromone signaling pathway; forms a complex
with Bud14p and Kel2p that regulates Bnr1p (formin) to
affect actin cable assembly, cytokinesis, and polarized
growth; functions in a complex with Kel2p to negatively
regulate mitotic exit, interacts with Tem1p and Lte1p;
localizes to regions of polarized growth; potential Cdc28p
substrate"
/codon_start=1
/product="Kel1p"
/protein_id="NP_012028.1"
/db_xref="GeneID:856563"
/db_xref="SGD:S000001201"
/translation="MAGFSFAKKFTHKKHGKTPSDASISDQSREASLSTPPNEKFFTK
QETPQKGRQFSQGYHSNVNKTSSPPMFARKQVSESRIQPSAVPPQQRNVSGPSTTLHK
QLSKQREYTVWNRIKLQNSPFPRYRHVASAYVTDKNQIYVIGGLHDQSVYGDTWILTA
FDNATRFSTTTIDISEATPPPRVGHAAVLCGNAFVVFGGDTHKVNKEGLMDDDIYLLN
INSYKWTVPAPVGPRPLGRYGHKISIIATTQMKTKLYVFGGQFDDTYFNDLAVYDLSS
FRRPDSHWEFLKPRTFTPPPITNFTMISYDSKLWVFGGDTLQGLVNDVFMYDPAINDW
FIIDTTGEKPPPVQEHATVVYNDLMCVVGGKDEHDAYLNSVYFLNLKSRKWFKLPVFT
AGIPQGRSGHSLTLLKNDKILIMGGDKFDYARVEEYDLHTSDIDMQRGTIVYTLDLAR
IKDLCPGVMDVPTDTPTPRNGNLDLATPVTPTSHQTKNMNVPISAAPLASAPSPAPKD
FSDADRLNREVHNRNVSTEHQNQSHPVNSESHLIAEPNILTPYVPSESSQTPVMKITS
NKPFDTPTIQKEPDLSETMDPTVGNQRIPSSIYGDNLTPANQIKNNSPILETLPSNEI
KTPQNGNIEEIKHLPDADEKIDSTTTFDQEINGDKLGTSSMSKVEEDGNVADEDDEIG
VAQMASSPSKDQFKIKHYNESSELSQNNTEIDKLSEPVDITIKKSDTAGHDSANHVID
ASDEKNVSPMGDVPTDTKNEEASVPINRDATTEVVDRALFEKLRSELQSLKELTHEKA
LEAGAHIKELETELWQLKSQKNSGTTKEIDELDSVRLQSKCEILEADNHSLEDKVNEL
EELVNSKFLDIENLNEVIQFQNEKIKSLELEPNYKEKLEELQIEHENLSRENERLKNE
SKQHNEDIINNVANYSSQLGSLISHWKENRANSSFLESSSSLISVSDENGEKTVGEPY
GDQSRHHRVVINKLTNRLDDLLERSQELTISKEKLSSEYHALKMEHSSLSQDVLVKEN
EIKKIQNDYKESISSMDSASKALMVSQRELEKYKSLNKKLIDELDELKFKNGVCSENF
ENGLRSTEESSNNVKNSNSIRENQFNIKINDLKAELFITNQERDDLKSEVLELKKRLL
NLENNTKQVNEDADSDLL"
gene <417549..>419063
/gene="TDA11"
/locus_tag="YHR159W"
/db_xref="GeneID:856564"
mRNA <417549..>419063
/gene="TDA11"
/locus_tag="YHR159W"
/product="Tda11p"
/transcript_id="NM_001179290.1"
/db_xref="GeneID:856564"
CDS 417549..419063
/gene="TDA11"
/locus_tag="YHR159W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to the cytoplasm; potential
Cdc28p substrate; null mutant is sensitive to expression
of the top1-T722A allele"
/codon_start=1
/product="Tda11p"
/protein_id="NP_012029.1"
/db_xref="GeneID:856564"
/db_xref="SGD:S000001202"
/translation="MNKFDEFIESNEKDLDVDTSTRNSIISMSPVRKTGRKIRSASSN
GYRLEHHRTSSAGSMHSQRLMTPTRLNDQDHPLQAKPDARRVVTRHSSVSVPNAMSKR
RSLIQPMVVPTTPESQNNLPSVSHSEGSYGIPLESTTVLSSEQAMASGLRRSRNGSSQ
SVNSMIATTIPTNGVDVSALLQSLATKELELLECKQKIEDLKKQTQHEEQNYTRRARE
LHELKEQVSKHLDPSLNTPVKNRAFSPVYQNIPLESRTENAGNSSLPSSVSKPKNMGH
QSTNQSRSVSPQDIQERRQRDDSSDSSKQSLWSKPLALFNQFDKIIQHEIERTLNWDD
SLSGTPEVQEGTPTSNSESSAQQYDNEAPGARQKSPSQGSVSRSLWSFVSDVKAGLLG
IEEENDNDVITDNRCDPVYKSDRQHEQKKSTHKITNRGQAEDSGDDSSLNMRKFKTTT
KFQKDNAGNNSLTDESGHRTREKKSKRSSNKLSFIGEPDNDNSSVKNSVEMTDF"
gene complement(<419221..>420072)
/gene="PEX18"
/locus_tag="YHR160C"
/db_xref="GeneID:856565"
mRNA complement(<419221..>420072)
/gene="PEX18"
/locus_tag="YHR160C"
/product="Pex18p"
/transcript_id="NM_001179291.1"
/db_xref="GeneID:856565"
CDS complement(419221..420072)
/gene="PEX18"
/locus_tag="YHR160C"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:9864360]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:9864360]"
/experiment="EXISTENCE:genetic interaction:GO:0016558
protein import into peroxisome matrix [PMID:9864360]"
/experiment="EXISTENCE:mutant phenotype:GO:0016558 protein
import into peroxisome matrix [PMID:9864360]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:9864360]"
/note="Peroxin; required for targeting of peroxisomal
matrix proteins containing PTS2; interacts with Pex7p;
partially redundant with Pex21p; primarily responsible for
peroxisomal import during growth on oleate, and expression
is induced during oleate growth"
/codon_start=1
/product="Pex18p"
/protein_id="NP_012030.1"
/db_xref="GeneID:856565"
/db_xref="SGD:S000001203"
/translation="MNSNRCQTNEVNKFISSTEKGPFTGRDNTLSFNKIGSRLNSPPI
LKDKIELKFLQHSEDLNQSRSYVNIRPRTLEDQSYKFEAPNLNDNETSWAKDFRYNFP
KNVEPPIENQIANLNINNGLRTSQTDFPLGFYSQKNFNIASFPVVDHQIFKTTGLEHP
INSHIDSLINAEFSELEASSLEEDVHTEEENSGTSLEDEETAMKGLASDIIEFCDNNS
ANKDVKERLNSSKFMGLMGSISDGSIVLKKDNGTERNLQKHVGFCFQNSGNWAGLEFH
DVEDRIA"
gene complement(<420373..>422286)
/gene="YAP1801"
/locus_tag="YHR161C"
/db_xref="GeneID:856566"
mRNA complement(<420373..>422286)
/gene="YAP1801"
/locus_tag="YHR161C"
/product="Yap1801p"
/transcript_id="NM_001179292.1"
/db_xref="GeneID:856566"
CDS complement(420373..422286)
/gene="YAP1801"
/locus_tag="YHR161C"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:33443082]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0030276 clathrin
binding [PMID:9531549]"
/experiment="EXISTENCE:physical interaction:GO:0006897
endocytosis [PMID:9531549]"
/note="Protein of the AP180 family, involved in clathrin
cage assembly; binds Pan1p and clathrin; YAP1801 has a
paralog, YAP1802, that arose from the whole genome
duplication"
/codon_start=1
/product="Yap1801p"
/protein_id="NP_012031.1"
/db_xref="GeneID:856566"
/db_xref="SGD:S000001204"
/translation="MTTYFKLVKGATKIKSAPPKQKYLDPILLGTSNEEDFYEIVKGL
DSRINDTAWTIVYKSLLVVHLMIREGSKDVALRYYSRNLEFFDIENIRGSNGSASGDM
RALDRYDNYLKVRCREFGKIKKDYVRDGYRTLKLNSGNYGSSRNKQHSINIALDHVES
LEVQIQALIKNKYTQYDLSNELIIFGFKLLIQDLLALYNALNEGIITLLESFFELSHH
NAERTLDLYKTFVDLTEHVVRYLKSGKTAGLKIPVIKHITTKLVRSLEEHLIEDDKTH
NTFVPVDSSQGSAGAVVAKSTAQERLEQIREQKRILEAQLKNEQVAISPALTTVTAAQ
SYNPFGTDSSMHTNIPMAVANQTQQIANNPFVSQTQPQVMNTPTAHTEPANLNVPEYA
AVQHTVNFNPVQDAGVSAQQTGYYSINNHLTPTFTGAGFGGYSVSQDTTAASNQQVSH
SQTGSNNPFALHNAATIATGNPAHENVLNNPFSRPNFDEQNTNMPLQQQIISNPFQNQ
TYNQQQFQQQKMPLSSINSVMTTPTSMQGSMNIPQRFDKMEFQAHYTQNHLQQQQQQQ
QQQQQQQQQQPQQGYYVPATAGANPVTNITGTVQPQNFPFYPQQQPQPEQSQTQQPVL
GNQYANNLNLIDM"
gene <423072..>423461
/gene="MPC2"
/locus_tag="YHR162W"
/db_xref="GeneID:856567"
mRNA <423072..>423461
/gene="MPC2"
/locus_tag="YHR162W"
/product="mitochondrial pyruvate carrier"
/transcript_id="NM_001179293.1"
/db_xref="GeneID:856567"
CDS 423072..423461
/gene="MPC2"
/locus_tag="YHR162W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:22628558]"
/experiment="EXISTENCE:direct assay:GO:0006850 pyruvate
import into mitochondria [PMID:25672363]"
/experiment="EXISTENCE:direct assay:GO:0050833 pyruvate
transmembrane transporter activity [PMID:25672363]"
/experiment="EXISTENCE:genetic interaction:GO:0006850
pyruvate import into mitochondria [PMID:22628554]"
/experiment="EXISTENCE:mutant phenotype:GO:0006850
pyruvate import into mitochondria [PMID:22628558]"
/experiment="EXISTENCE:physical interaction:GO:0050833
pyruvate transmembrane transporter activity
[PMID:22628558]"
/note="Highly conserved subunit of the mitochondrial
pyruvate carrier (MPC); expressed during growth on
fermentable carbon sources, and heterodimerizes with Mpc1p
to form the fermentative isoform of MPC; MPC localizes to
the mitochondrial inner membrane and mediates pyruvate
uptake; MPC2 paralog, MPC3, heterodimerizes with Mpc1p to
form the respiratory MPC isoform"
/codon_start=1
/product="mitochondrial pyruvate carrier"
/protein_id="NP_012032.1"
/db_xref="GeneID:856567"
/db_xref="SGD:S000001205"
/translation="MSTSSVRFAFRRFWQSETGPKTVHFWAPTLKWGLVFAGFSDMKR
PVEKISGAQNLSLLSTALIWTRWSFVIKPRNILLASVNSFLCLTAGYQLGRIANYRIR
NGDSISQLCSYILSGADESKKEITTGR"
gene <423724..>424473
/gene="SOL3"
/locus_tag="YHR163W"
/db_xref="GeneID:856568"
mRNA <423724..>424473
/gene="SOL3"
/locus_tag="YHR163W"
/product="6-phosphogluconolactonase SOL3"
/transcript_id="NM_001179294.1"
/db_xref="GeneID:856568"
CDS 423724..424473
/gene="SOL3"
/locus_tag="YHR163W"
/EC_number="3.1.1.31"
/experiment="EXISTENCE:curator inference:GO:0009051
pentose-phosphate shunt, oxidative branch [PMID:15454531]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0017057
6-phosphogluconolactonase activity [PMID:15454531]"
/note="6-phosphogluconolactonase; catalyzes the second
step of the pentose phosphate pathway; weak multicopy
suppressor of los1-1 mutation; homologous to Sol2p and
Sol1p; SOL3 has a paralog, SOL4, that arose from the whole
genome duplication"
/codon_start=1
/product="6-phosphogluconolactonase SOL3"
/protein_id="NP_012033.2"
/db_xref="GeneID:856568"
/db_xref="SGD:S000001206"
/translation="MVTVGVFSERASLTHQLGEFIVKKQDEALQKKSDFKVSVSGGSL
IDALYESLVADESLSSRVQWSKWQIYFSDERIVPLTDADSNYGAFKRAVLDKLPSTSQ
PNVYPMDESLIGSDAESNNKIAAEYERIVPQVLDLVLLGCGPDGHTCSLFPGETHRYL
LNETTKRVAWCHDSPKPPSDRITFTLPVLKDAKALCFVAEGSSKQNIMHEIFDLKNDQ
LPTALVNKLFGEKTSWFVNEEAFGKVQTKTF"
gene complement(<424610..>429178)
/gene="DNA2"
/locus_tag="YHR164C"
/gene_synonym="WEB2"
/db_xref="GeneID:856569"
mRNA complement(<424610..>429178)
/gene="DNA2"
/locus_tag="YHR164C"
/gene_synonym="WEB2"
/product="bifunctional ATP-dependent DNA helicase/ssDNA
endodeoxyribonuclease DNA2"
/transcript_id="NM_001179295.1"
/db_xref="GeneID:856569"
CDS complement(424610..429178)
/gene="DNA2"
/locus_tag="YHR164C"
/gene_synonym="WEB2"
/EC_number="3.6.4.12"
/experiment="EXISTENCE:direct assay:GO:0000014
single-stranded DNA endodeoxyribonuclease activity
[PMID:9756935|PMID:23671118]"
/experiment="EXISTENCE:direct assay:GO:0000400 four-way
junction DNA binding [PMID:32638513]"
/experiment="EXISTENCE:direct assay:GO:0000781 chromosome,
telomeric region [PMID:12024033]"
/experiment="EXISTENCE:direct assay:GO:0003678 DNA
helicase activity [PMID:7644470]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922|PMID:19520826]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:19520826]"
/experiment="EXISTENCE:direct assay:GO:0006261
DNA-templated DNA replication [PMID:9710536]"
/experiment="EXISTENCE:direct assay:GO:0017108 5'-flap
endonuclease activity [PMID:15448135]"
/experiment="EXISTENCE:direct assay:GO:0032448 DNA hairpin
binding [PMID:23344960]"
/experiment="EXISTENCE:direct assay:GO:0043539 protein
serine/threonine kinase activator activity
[PMID:23355394]"
/experiment="EXISTENCE:genetic interaction:GO:0000706
meiotic DNA double-strand break processing
[PMID:20150422]"
/experiment="EXISTENCE:genetic interaction:GO:0000729 DNA
double-strand break processing [PMID:21841787]"
/experiment="EXISTENCE:genetic interaction:GO:0033314
mitotic DNA replication checkpoint signaling
[PMID:23355394]"
/experiment="EXISTENCE:mutant phenotype:GO:0000723
telomere maintenance [PMID:12024033]"
/experiment="EXISTENCE:mutant phenotype:GO:0000729 DNA
double-strand break processing [PMID:18805091]"
/experiment="EXISTENCE:mutant phenotype:GO:0006261
DNA-templated DNA replication [PMID:7592912]"
/experiment="EXISTENCE:mutant phenotype:GO:0006273 lagging
strand elongation [PMID:10101169|PMID:10330154]"
/experiment="EXISTENCE:mutant phenotype:GO:0006281 DNA
repair [PMID:10101169]"
/experiment="EXISTENCE:mutant phenotype:GO:0032448 DNA
hairpin binding [PMID:23344960]"
/experiment="EXISTENCE:mutant phenotype:GO:0043539 protein
serine/threonine kinase activator activity
[PMID:23355394]"
/note="ATP-dependent 5'-flap endonuclease and DNA
helicase; tracking protein for flap cleavage during
Okazaki fragment maturation; involved in DNA
repair/processing of meiotic DNA double strand breaks;
cell cycle-dependent localization to telomeric chromatin
and replicating chromosomes; required for
telomerase-dependent telomere synthesis; stability,
localization and nuclease activity regulated by
sumoylation; forms nuclear foci upon replication stress;
human homolog DNA2 complements yeast dna2 mutant"
/codon_start=1
/product="bifunctional ATP-dependent DNA helicase/ssDNA
endodeoxyribonuclease DNA2"
/protein_id="NP_012034.1"
/db_xref="GeneID:856569"
/db_xref="SGD:S000001207"
/translation="MPGTPQKNKRSASISVSPAKKTEEKEIIQNDSKAILSKQTKRKK
KYAFAPINNLNGKNTKVSNASVLKSIAVSQVRNTSRTKDINKAVSKSVKQLPNSQVKP
KREMSNLSRHHDFTQDEDGPMEEVIWKYSPLQRDMSDKTTSAAEYSDDYEDVQNPSST
PIVPNRLKTVLSFTNIQVPNADVNQLIQENGNEQVRPKPAEISTRESLRNIDDILDDI
EGDLTIKPTITKFSDLPSSPIKAPNVEKKAEVNAEEVDKMDSTGDSNDGDDSLIDILT
QKYVEKRKSESQITIQGNTNQKSGAQESCGKNDNTKSRGEIEDHENVDNQAKTGNAFY
ENEEDSNCQRIKKNEKIEYNSSDEFSDDSLIELLNETQTQVEPNTIEQDLDKVEKMVS
DDLRIATDSTLSAYALRAKSGAPRDGVVRLVIVSLRSVELPKIGTQKILECIDGKGEQ
SSVVVRHPWVYLEFEVGDVIHIIEGKNIENKRLLSDDKNPKTQLANDNLLVLNPDVLF
SATSVGSSVGCLRRSILQMQFQDPRGEPSLVMTLGNIVHELLQDSIKYKLSHNKISME
IIIQKLDSLLETYSFSIIICNEEIQYVKELVMKEHAENILYFVNKFVSKSNYGCYTSI
SGTRRTQPISISNVIDIEENIWSPIYGLKGFLDATVEANVENNKKHIVPLEVKTGKSR
SVSYEVQGLIYTLLLNDRYEIPIEFFLLYFTRDKNMTKFPSVLHSIKHILMSRNRMSM
NFKHQLQEVFGQAQSRFELPPLLRDSSCDSCFIKESCMVLNKLLEDGTPEESGLVEGE
FEILTNHLSQNLANYKEFFTKYNDLITKEESSITCVNKELFLLDGSTRESRSGRCLSG
LVVSEVVEHEKTEGAYIYCFSRRRNDNNSQSMLSSQIAANDFVIISDEEGHFCLCQGR
VQFINPAKIGISVKRKLLNNRLLDKEKGVTTIQSVVESELEQSSLIATQNLVTYRIDK
NDIQQSLSLARFNLLSLFLPAVSPGVDIVDERSKLCRKTKRSDGGNEILRSLLVDNRA
PKFRDANDDPVIPYKLSKDTTLNLNQKEAIDKVMRAEDYALILGMPGTGKTTVIAEII
KILVSEGKRVLLTSYTHSAVDNILIKLRNTNISIMRLGMKHKVHPDTQKYVPNYASVK
SYNDYLSKINSTSVVATTCLGINDILFTLNEKDFDYVILDEASQISMPVALGPLRYGN
RFIMVGDHYQLPPLVKNDAARLGGLEESLFKTFCEKHPESVAELTLQYRMCGDIVTLS
NFLIYDNKLKCGNNEVFAQSLELPMPEALSRYRNESANSKQWLEDILEPTRKVVFLNY
DNCPDIIEQSEKDNITNHGEAELTLQCVEGMLLSGVPCEDIGVMTLYRAQLRLLKKIF
NKNVYDGLEILTADQFQGRDKKCIIISMVRRNSQLNGGALLKELRRVNVAMTRAKSKL
IIIGSKSTIGSVPEIKSFVNLLEERNWVYTMCKDALYKYKFPDRSNAIDEARKGCGKR
TGAKPITSKSKFVSDKPIIKEILQEYES"
gene complement(<429707..>436948)
/gene="PRP8"
/locus_tag="YHR165C"
/gene_synonym="DBF3; DNA39; RNA8; SLT21; USA2"
/db_xref="GeneID:856570"
mRNA complement(<429707..>436948)
/gene="PRP8"
/locus_tag="YHR165C"
/gene_synonym="DBF3; DNA39; RNA8; SLT21; USA2"
/product="U4/U6-U5 snRNP complex subunit PRP8"
/transcript_id="NM_001179296.1"
/db_xref="GeneID:856570"
CDS complement(429707..436948)
/gene="PRP8"
/locus_tag="YHR165C"
/gene_synonym="DBF3; DNA39; RNA8; SLT21; USA2"
/experiment="EXISTENCE:direct assay:GO:0000244
spliceosomal tri-snRNP complex assembly [PMID:23393194]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:17934474|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005682 U5 snRNP
[PMID:17934474|PMID:11720284|PMID:11720285]"
/experiment="EXISTENCE:direct assay:GO:0017070 U6 snRNA
binding [PMID:23393194]"
/experiment="EXISTENCE:direct assay:GO:0030619 U1 snRNA
binding [PMID:23393194]"
/experiment="EXISTENCE:direct assay:GO:0030620 U2 snRNA
binding [PMID:23393194]"
/experiment="EXISTENCE:direct assay:GO:0030623 U5 snRNA
binding [PMID:23393194|PMID:9848662]"
/experiment="EXISTENCE:direct assay:GO:0046540 U4/U6 x U5
tri-snRNP complex
[PMID:10449419|PMID:10377396|PMID:17934474]"
/experiment="EXISTENCE:direct assay:GO:0097157 pre-mRNA
intronic binding [PMID:23393194]"
/experiment="EXISTENCE:mutant phenotype:GO:0000244
spliceosomal tri-snRNP complex assembly [PMID:17934474]"
/experiment="EXISTENCE:mutant phenotype:GO:0000350
generation of catalytic spliceosome for second
transesterification step [PMID:22408182|PMID:26968627]"
/experiment="EXISTENCE:mutant phenotype:GO:0000386 second
spliceosomal transesterification activity [PMID:17626844]"
/experiment="EXISTENCE:mutant phenotype:GO:0000389 mRNA
3'-splice site recognition [PMID:10628969]"
/experiment="EXISTENCE:mutant phenotype:GO:0000395 mRNA
5'-splice site recognition [PMID:29487104]"
/note="Component of U4/U6-U5 snRNP complex; involved in
second catalytic step of splicing; participates in
spliceosomal assembly through its interaction with U1
snRNA; largest and most evolutionarily conserved protein
of the spliceosome; mutations in human ortholog, PRPF8,
cause Retinitis pigmentosa and missplicing in
Myelodysplastic syndrome; mouse ortholog interacts with
androgen receptor and may have a role in prostate cancer"
/codon_start=1
/product="U4/U6-U5 snRNP complex subunit PRP8"
/protein_id="NP_012035.1"
/db_xref="GeneID:856570"
/db_xref="SGD:S000001208"
/translation="MSGLPPPPPGFEEDSDLALPPPPPPPPGYEIEELDNPMVPSSVN
EDTFLPPPPPPPSNFEINAEEIVDFTLPPPPPPPGLDELETKAEKKVELHGKRKLDIG
KDTFVTRKSRKRAKKMTKKAKRSNLYTPKAEMPPEHLRKIINTHSDMASKMYNTDKKA
FLGALKYLPHAILKLLENMPHPWEQAKEVKVLYHTSGAITFVNETPRVIEPVYTAQWS
ATWIAMRREKRDRTHFKRMRFPPFDDDEPPLSYEQHIENIEPLDPINLPLDSQDDEYV
KDWLYDSRPLEEDSKKVNGTSYKKWSFDLPEMSNLYRLSTPLRDEVTDKNYYYLFDKK
SFFNGKALNNAIPGGPKFEPLYPREEEEDYNEFNSIDRVIFRVPIRSEYKVAFPHLYN
SRPRSVRIPWYNNPVSCIIQNDEEYDTPALFFDPSLNPIPHFIDNNSSLNVSNTKENG
DFTLPEDFAPLLAEEEELILPNTKDAMSLYHSPFPFNRTKGKMVRAQDVALAKKWFLQ
HPDEEYPVKVKVSYQKLLKNYVLNELHPTLPTNHNKTKLLKSLKNTKYFQQTTIDWVE
AGLQLCRQGHNMLNLLIHRKGLTYLHLDYNFNLKPTKTLTTKERKKSRLGNSFHLMRE
LLKMMKLIVDTHVQFRLGNVDAFQLADGIHYILNHIGQLTGIYRYKYKVMHQIRACKD
LKHIIYYKFNKNLGKGPGCGFWQPAWRVWLNFLRGTIPLLERYIGNLITRQFEGRSNE
IVKTTTKQRLDAYYDLELRNSVMDDILEMMPESIRQKKARTILQHLSEAWRCWKANIP
WDVPGMPAPIKKIIERYIKSKADAWVSAAHYNRERIKRGAHVEKTMVKKNLGRLTRLW
IKNEQERQRQIQKNGPEITPEEATTIFSVMVEWLESRSFSPIPFPPLTYKNDTKILVL
ALEDLKDVYASKVRLNASEREELALIEEAYDNPHDTLNRIKKYLLTQRVFKPVDITMM
ENYQNISPVYSVDPLEKITDAYLDQYLWYEADQRKLFPNWIKPSDSEIPPLLVYKWTQ
GINNLSEIWDVSRGQSAVLLETTLGEMAEKIDFTLLNRLLRLIVDPNIADYITAKNNV
VINFKDMSHVNKYGLIRGLKFASFIFQYYGLVIDLLLLGQERATDLAGPANNPNEFMQ
FKSKEVEKAHPIRLYTRYLDRIYMLFHFEEDEGEELTDEYLAENPDPNFENSIGYNNR
KCWPKDSRMRLIRQDVNLGRAVFWEIQSRVPTSLTSIKWENAFVSVYSKNNPNLLFSM
CGFEVRILPRQRMEEVVSNDEGVWDLVDERTKQRTAKAYLKVSEEEIKKFDSRIRGIL
MASGSTTFTKVAAKWNTSLISLFTYFREAIVATEPLLDILVKGETRIQNRVKLGLNSK
MPTRFPPAVFYTPKELGGLGMISASHILIPASDLSWSKQTDTGITHFRAGMTHEDEKL
IPTIFRYITTWENEFLDSQRVWAEYATKRQEAIQQNRRLAFEELEGSWDRGIPRISTL
FQRDRHTLAYDRGHRIRREFKQYSLERNSPFWWTNSHHDGKLWNLNAYRTDVIQALGG
IETILEHTLFKGTGFNSWEGLFWEKASGFEDSMQFKKLTHAQRTGLSQIPNRRFTLWW
SPTINRANVYVGFLVQLDLTGIFLHGKIPTLKISLIQIFRAHLWQKIHESIVFDICQI
LDGELDVLQIESVTKETVHPRKSYKMNSSAADITMESVHEWEVSKPSLLHETNDSFKG
LITNKMWFDVQLRYGDYDSHDISRYVRAKFLDYTTDNVSMYPSPTGVMIGIDLAYNMY
DAYGNWFNGLKPLIQNSMRTIMKANPALYVLRERIRKGLQIYQSSVQEPFLNSSNYAE
LFNNDIKLFVDDTNVYRVTVHKTFEGNVATKAINGCIFTLNPKTGHLFLKIIHTSVWA
GQKRLSQLAKWKTAEEVSALVRSLPKEEQPKQIIVTRKAMLDPLEVHMLDFPNIAIRP
TELRLPFSAAMSIDKLSDVVMKATEPQMVLFNIYDDWLDRISSYTAFSRLTLLLRALK
TNEESAKMILLSDPTITIKSYHLWPSFTDEQWITIESQMRDLILTEYGRKYNVNISAL
TQTEIKDIILGQNIKAPSVKRQKMAELEAARSEKQNDEEAAGASTVMKTKTINAQGEE
IVVVASADYESQTFSSKNEWRKSAIANTLLYLRLKNIYVSADDFVEEQNVYVLPKNLL
KKFIEISDVKIQVAAFIYGMSAKDHPKVKEIKTVVLVPQLGHVGSVQISNIPDIGDLP
DTEGLELLGWIHTQTEELKFMAASEVATHSKLFADKKRDCIDISIFSTPGSVSLSAYN
LTDEGYQWGEENKDIMNVLSEGFEPTFSTHAQLLLSDRITGNFIIPSGNVWNYTFMGT
AFNQEGDYNFKYGIPLEFYNEMHRPVHFLQFSELAGDEELEAEQIDVFS"
gene complement(<437170..>439050)
/gene="CDC23"
/locus_tag="YHR166C"
/db_xref="GeneID:856571"
mRNA complement(<437170..>439050)
/gene="CDC23"
/locus_tag="YHR166C"
/product="anaphase promoting complex subunit CDC23"
/transcript_id="NM_001179297.1"
/db_xref="GeneID:856571"
CDS complement(437170..439050)
/gene="CDC23"
/locus_tag="YHR166C"
/experiment="EXISTENCE:direct assay:GO:0005680
anaphase-promoting complex [PMID:9469814]"
/experiment="EXISTENCE:direct assay:GO:0016567 protein
ubiquitination [PMID:16481473]"
/experiment="EXISTENCE:direct assay:GO:0031145
anaphase-promoting complex-dependent catabolic process
[PMID:8895471]"
/experiment="EXISTENCE:mutant phenotype:GO:0016567 protein
ubiquitination [PMID:16481473]"
/experiment="EXISTENCE:mutant phenotype:GO:0031145
anaphase-promoting complex-dependent catabolic process
[PMID:16481473]"
/experiment="EXISTENCE:mutant phenotype:GO:0061630
ubiquitin protein ligase activity [PMID:16481473]"
/experiment="EXISTENCE:physical interaction:GO:0030332
cyclin binding [PMID:12413490]"
/note="Subunit of the Anaphase-Promoting Complex/Cyclosome
(APC/C); APC/C is a ubiquitin-protein ligase required for
degradation of anaphase inhibitors, including mitotic
cyclins, during the metaphase/anaphase transition"
/codon_start=1
/product="anaphase promoting complex subunit CDC23"
/protein_id="NP_012036.1"
/db_xref="GeneID:856571"
/db_xref="SGD:S000001209"
/translation="MNDDSQDKIIHDIRIQLRKAATELSRWKLYGSSKWAAEALAGLA
EAIDVDQTHSLADESPLRNKQGVPKQMFEIPQNGFGLSETEYDLYLLGSTLFDAKEFD
RCVFFLKDVTNPYLKFLKLYSKFLSWDKKSQESMENILTTGKFTDEMYRANKDGDGSG
NEDINQSGHQRANLKMVSNEHESQSNISSILKEINTFLESYEIKIDDDEADLGLALLY
YLRGVILKQEKNISKAMSSFLKSLSCYSFNWSCWLELMDCLQKVDDALLLNNYLYQNF
QFKFSENLGSQRTIEFNIMIKFFKLKVFEELNGQLEDYFEDLEFLLQVFPNFTFLKAY
NATISYNNLDYVTAESRFDDIVKQDPYRLNDLETYSNILYVMQKNSKLAYLAQFVSQI
DRFRPETCCIIANYYSARQEHEKSIMYFRRALTLDKKTTNAWTLMGHEFVELSNSHAA
IECYRRAVDICPRDFKAWFGLGQAYALLDMHLYSLYYFQKACTLKPWDRRIWQVLGEC
YSKTGNKVEAIKCYKRSIKASQTVDQNTSIYYRLAQLYEELEDLQECKKFMMKCVDVE
ELLEGIVTDETVKARLWLAIFEIKAGNYQLAYDYAMGVSSGTSQEIEEARMLARECRR
HM"
gene <439342..>440127
/gene="THP2"
/locus_tag="YHR167W"
/db_xref="GeneID:856572"
mRNA <439342..>440127
/gene="THP2"
/locus_tag="YHR167W"
/product="Thp2p"
/transcript_id="NM_001179298.1"
/db_xref="GeneID:856572"
CDS 439342..440127
/gene="THP2"
/locus_tag="YHR167W"
/experiment="EXISTENCE:direct assay:GO:0003676 nucleic
acid binding [PMID:12093753]"
/experiment="EXISTENCE:mutant phenotype:GO:0000445 THO
complex part of transcription export complex
[PMID:11979277]"
/experiment="EXISTENCE:mutant phenotype:GO:0000446
nucleoplasmic THO complex [PMID:11060033]"
/experiment="EXISTENCE:mutant phenotype:GO:0006310 DNA
recombination [PMID:11060033]"
/experiment="EXISTENCE:mutant phenotype:GO:0006368
transcription elongation by RNA polymerase II
[PMID:12093753]"
/experiment="EXISTENCE:mutant phenotype:GO:0006406 mRNA
export from nucleus [PMID:11979277]"
/experiment="EXISTENCE:mutant phenotype:GO:0034063 stress
granule assembly [PMID:27251550]"
/experiment="EXISTENCE:mutant phenotype:GO:0097185
cellular response to azide [PMID:27251550]"
/note="Subunit of the THO and TREX complexes; THO connects
transcription elongation and mitotic recombination, and
TREX is recruited to activated genes and couples
transcription to mRNA export; involved in telomere
maintenance"
/codon_start=1
/product="Thp2p"
/protein_id="NP_012037.1"
/db_xref="GeneID:856572"
/db_xref="SGD:S000001210"
/translation="MTKEEGRTYFESLCEEEQSLQESQTHLLNILDILSVLADPRSSD
DLLTESLKKLPDLHRELINSSIRLRYDKYQTREAQLLEDTKTGRDVAAGVQNPKSISE
YYSTFEHLNRDTLRYINLLKRLSVDLAKQVEVSDPSVTVYEMDKWVPSEKLQGILEQY
CAPDTDIRGVDAQIKNYLDQIKMARAKFGLENKYSLKERLSTLTKELNHWRKEWDDIE
MLMFGDDAHSMKKMIQKIDSLKSEINAPSESYPVDKEGDIVLE"
gene <440377..>441933
/gene="MTG2"
/locus_tag="YHR168W"
/db_xref="GeneID:856573"
mRNA <440377..>441933
/gene="MTG2"
/locus_tag="YHR168W"
/product="putative GTPase MTG2"
/transcript_id="NM_001179299.2"
/db_xref="GeneID:856573"
CDS 440377..441933
/gene="MTG2"
/locus_tag="YHR168W"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:15591131]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:15591131]"
/experiment="EXISTENCE:mutant phenotype:GO:1902775
mitochondrial large ribosomal subunit assembly
[PMID:15591131]"
/note="Putative GTPase; member of the Obg family;
peripheral protein of the mitochondrial inner membrane
that associates with the large ribosomal subunit; required
for mitochondrial translation, possibly via a role in
ribosome assembly"
/codon_start=1
/product="putative GTPase MTG2"
/protein_id="NP_012038.2"
/db_xref="GeneID:856573"
/db_xref="SGD:S000001211"
/translation="MSIAWSSVFKRELRLERFLPRVYSTKVPDNAPRAADNEQWLETL
RPITHPEQKKSDHDVSYTRHINVPLGEVTSVNYLQRYNKHKHSQGNFVDVRIVKCKSG
AGGSGAVSFFRDAGRSIGPPDGGDGGAGGSVYIQAVAGLGSLAKMKTTYTAEDGEAGA
ARQLDGMRGRDVLIQVPVGTVVKWCLPPQKVRELVEREMRKDNNATLRSILGSTAVNL
SVSSGSHRKKIQLYRHEMAESWLFKDKAKEYHENKDWFKDLHKKMEAYDHSLEQSELF
NDQFPLAGLDLNQPMTKPVCLLKGGQGGLGNMHFLTNLIRNPRFSKPGRNGLEQHFLF
ELKSIADLGLIGLPNAGKSTILNKISNAKPKIGHWQFTTLSPTIGTVSLGFGQDVFTV
ADIPGIIQGASLDKGMGLEFLRHIERSNGWVFVLDLSNKNPLNDLQLLIEEVGTLEKV
KTKNILIVCNKVDIDAEKSESFAKYLQVEKFSKSQEWDCVPISALREENIDVLKKKMF
KCARQSEFDK"
gene <442181..>443476
/gene="DBP8"
/locus_tag="YHR169W"
/db_xref="GeneID:856574"
mRNA <442181..>443476
/gene="DBP8"
/locus_tag="YHR169W"
/product="ATP-dependent RNA helicase DBP8"
/transcript_id="NM_001179300.1"
/db_xref="GeneID:856574"
CDS 442181..443476
/gene="DBP8"
/locus_tag="YHR169W"
/EC_number="3.6.4.13"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:11222764]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:16772403]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/experiment="EXISTENCE:mutant phenotype:GO:0000447
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from
5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11222764]"
/experiment="EXISTENCE:mutant phenotype:GO:0000472
endonucleolytic cleavage to generate mature 5'-end of
SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:11222764]"
/experiment="EXISTENCE:mutant phenotype:GO:0000480
endonucleolytic cleavage in 5'-ETS of tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:11222764]"
/experiment="EXISTENCE:mutant phenotype:GO:0003724 RNA
helicase activity [PMID:11222764]"
/note="ATPase, putative RNA helicase of the DEAD-box
family; component of 90S preribosome complex involved in
production of 18S rRNA and assembly of 40S small ribosomal
subunit; ATPase activity stimulated by association with
Esf2p"
/codon_start=1
/product="ATP-dependent RNA helicase DBP8"
/protein_id="NP_012039.1"
/db_xref="GeneID:856574"
/db_xref="SGD:S000001212"
/translation="MADFKSLGLSKWLTESLRAMKITQPTAIQKACIPKILEGRDCIG
GAKTGSGKTIAFAGPMLTKWSEDPSGMFGVVLTPTRELAMQIAEQFTALGSSMNIRVS
VIVGGESIVQQALDLQRKPHFIIATPGRLAHHIMSSGDDTVGGLMRAKYLVLDEADIL
LTSTFADHLATCISALPPKDKRQTLLFTATITDQVKSLQNAPVQKGKPPLFAYQVESV
DNVAIPSTLKIEYILVPEHVKEAYLYQLLTCEEYENKTAIIFVNRTMTAEILRRTLKQ
LEVRVASLHSQMPQQERTNSLHRFRANAARILIATDVASRGLDIPTVELVVNYDIPSD
PDVFIHRSGRTARAGRIGDAISFVTQRDVSRIQAIEDRINKKMTETNKVHDTAVIRKA
LTKVTKAKRESLMAMQKENFGERKRQQKKKQNDGKSLRS"
gene <443828..>445384
/gene="NMD3"
/locus_tag="YHR170W"
/gene_synonym="SRC5"
/db_xref="GeneID:856575"
mRNA <443828..>445384
/gene="NMD3"
/locus_tag="YHR170W"
/gene_synonym="SRC5"
/product="ribosome-binding protein NMD3"
/transcript_id="NM_001179301.1"
/db_xref="GeneID:856575"
CDS 443828..445384
/gene="NMD3"
/locus_tag="YHR170W"
/gene_synonym="SRC5"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:10022925]"
/experiment="EXISTENCE:direct assay:GO:0030674
protein-macromolecule adaptor activity
[PMID:11313466|PMID:11086007]"
/experiment="EXISTENCE:direct assay:GO:0043023 ribosomal
large subunit binding [PMID:17347149|PMID:11105761]"
/experiment="EXISTENCE:direct assay:GO:0070180 large
ribosomal subunit rRNA binding [PMID:24240281]"
/experiment="EXISTENCE:genetic interaction:GO:0000055
ribosomal large subunit export from nucleus
[PMID:23212245]"
/experiment="EXISTENCE:mutant phenotype:GO:0000055
ribosomal large subunit export from nucleus
[PMID:11086007]"
/note="Protein involved in nuclear export of the large
ribosomal subunit; acts as a Crm1p-dependent adapter
protein for export of nascent ribosomal subunits through
the nuclear pore complex"
/codon_start=1
/product="ribosome-binding protein NMD3"
/protein_id="NP_012040.1"
/db_xref="GeneID:856575"
/db_xref="SGD:S000001213"
/translation="MEFTPIDPHQHQNAATLLCCNCGTPIDGSTGLVMCYDCIKLTVD
ITQGIPREANISFCRNCERFLQPPGQWIRAELESRELLAICLRRLKGLTKVRLVDASF
IWTEPHSRRIRIKLTVQGEAMTNTIIQQTFEVEYIVIAMQCPDCARSYTTNTWRATVQ
IRQKVPHKRTFLFLEQLILKHNAHVDTISISEAKDGLDFFYAQKNHAVKMIDFLNAVV
PIKHKKSEELISQDTHTGASTYKFSYSVEIVPICKDDLVVLPKKLAKSMGNISQFVLC
SKISNTVQFMDPTTLQTADLSPSVYWRAPFNALADVTQLVEFIVLDVDSTGISRGNRV
LADITVARTSDLGVNDQVYYVRSHLGGICHAGDSVMGYFIANSNYNSDLFDGLNIDYV
PDVVLVKKLYQRKSKKSRHWKLKRMAKEHKDIDASLDYNSRAQKQEMERAEKDYELFL
QELEEDAELRQSVNLYKNREANVPPEEHEMDEDEDEDAPQINIDELLDELDEMTLEDG
VENTPVESQQ"
gene <445713..>447605
/gene="ATG7"
/locus_tag="YHR171W"
/gene_synonym="APG11; APG7; CVT2"
/db_xref="GeneID:856576"
mRNA <445713..>447605
/gene="ATG7"
/locus_tag="YHR171W"
/gene_synonym="APG11; APG7; CVT2"
/product="Atg7p"
/transcript_id="NM_001179302.1"
/db_xref="GeneID:856576"
CDS 445713..447605
/gene="ATG7"
/locus_tag="YHR171W"
/gene_synonym="APG11; APG7; CVT2"
/experiment="EXISTENCE:direct assay:GO:0000407 phagophore
assembly site [PMID:18497569]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762|PMID:10233150]"
/experiment="EXISTENCE:direct assay:GO:0006501 C-terminal
protein lipidation [PMID:19398890|PMID:15277523]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:10233148]"
/experiment="EXISTENCE:mutant phenotype:GO:0000422
autophagy of mitochondrion [PMID:19793921]"
/experiment="EXISTENCE:mutant phenotype:GO:0006501
C-terminal protein lipidation
[PMID:15277523|PMID:12965207|PMID:11100732|PMID:11038174]"
/experiment="EXISTENCE:mutant phenotype:GO:0006914
autophagy [PMID:23382696]"
/experiment="EXISTENCE:mutant phenotype:GO:0016236
macroautophagy [PMID:10233150|PMID:12965207]"
/experiment="EXISTENCE:mutant phenotype:GO:0019778 Atg12
activating enzyme activity [PMID:10233150]"
/experiment="EXISTENCE:mutant phenotype:GO:0019779 Atg8
activating enzyme activity [PMID:11100732]"
/experiment="EXISTENCE:mutant phenotype:GO:0032258
cytoplasm to vacuole targeting by the Cvt pathway
[PMID:10233150|PMID:12965207]"
/experiment="EXISTENCE:mutant phenotype:GO:0032446 protein
modification by small protein conjugation [PMID:10233150]"
/experiment="EXISTENCE:mutant phenotype:GO:0034727
piecemeal microautophagy of the nucleus [PMID:18701704]"
/experiment="EXISTENCE:mutant phenotype:GO:0044804
nucleophagy [PMID:22768199]"
/note="Autophagy-related protein and dual specificity
member of the E1 family; mediates the attachment of Atg12p
to Atg5p and Atg8p to phosphatidylethanolamine which are
required steps in autophagosome formation; E1 enzymes are
also known as ubiquitin-activating enzymes; involved in
methionine restriction extension of chronological lifespan
in an autophagy-dependent manner"
/codon_start=1
/product="Atg7p"
/protein_id="NP_012041.1"
/db_xref="GeneID:856576"
/db_xref="SGD:S000001214"
/translation="MSSERVLSYAPAFKSFLDTSFFQELSRLKLDVLKLDSTCQPLTV
NLDLHNIPKSADQVPLFLTNRSFEKHNNKRTNEVPLQGSIFNFNVLDEFKNLDKQLFL
HQRALECWEDGIKDINKCVSFVIISFADLKKYRFYYWLGVPCFQRPSSTVLHVRPEPS
LKGLFSKCQKWFDVNYSKWVCILDADDEIVNYDKCIIRKTKVLAIRDTSTMENVPSAL
TKNFLSVLQYDVPDLIDFKLLIIRQNEGSFALNATFASIDPQSSSSNPDMKVSGWERN
VQGKLAPRVVDLSSLLDPLKIADQSVDLNLKLMKWRILPDLNLDIIKNTKVLLLGAGT
LGCYVSRALIAWGVRKITFVDNGTVSYSNPVRQALYNFEDCGKPKAELAAASLKRIFP
LMDATGVKLSIPMIGHKLVNEEAQHKDFDRLRALIKEHDIIFLLVDSRESRWLPSLLS
NIENKTVINAALGFDSYLVMRHGNRDEQSSKQLGCYFCHDVVAPTDSLTDRTLDQMCT
VTRPGVAMMASSLAVELMTSLLQTKYSGSETTVLGDIPHQIRGFLHNFSILKLETPAY
EHCPACSPKVIEAFTDLGWEFVKKALEHPLYLEEISGLSVIKQEVERLGNDVFEWEDD
ESDEIA"
rep_origin 447714..447795
/note="ARS820; Autonomously Replicating Sequence"
/db_xref="SGD:S000118361"
gene <448335..>450806
/gene="SPC97"
/locus_tag="YHR172W"
/db_xref="GeneID:856577"
mRNA <448335..>450806
/gene="SPC97"
/locus_tag="YHR172W"
/product="gamma-tubulin-complex subunit SPC97"
/transcript_id="NM_001179303.1"
/db_xref="GeneID:856577"
CDS 448335..450806
/gene="SPC97"
/locus_tag="YHR172W"
/experiment="EXISTENCE:direct assay:GO:0005816 spindle
pole body [PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005822 inner
plaque of spindle pole body [PMID:9215630|PMID:9130700]"
/experiment="EXISTENCE:direct assay:GO:0005824 outer
plaque of spindle pole body [PMID:9215630|PMID:9130700]"
/experiment="EXISTENCE:direct assay:GO:0007020 microtubule
nucleation [PMID:11950928|PMID:24842996]"
/experiment="EXISTENCE:direct assay:GO:0008275
gamma-tubulin small complex
[PMID:9130700|PMID:9384578|PMID:25599398]"
/experiment="EXISTENCE:mutant phenotype:GO:0031122
cytoplasmic microtubule organization [PMID:9670012]"
/experiment="EXISTENCE:physical interaction:GO:0008275
gamma-tubulin small complex [PMID:9130700]"
/experiment="EXISTENCE:physical interaction:GO:0043015
gamma-tubulin binding [PMID:9130700]"
/note="Component of the microtubule-nucleating Tub4p
(gamma-tubulin) complex; interacts with Spc110p at the
spindle pole body (SPB) inner plaque and with Spc72p at
the SPB outer plaque"
/codon_start=1
/product="gamma-tubulin-complex subunit SPC97"
/protein_id="NP_012042.1"
/db_xref="GeneID:856577"
/db_xref="SGD:S000001215"
/translation="MEIKEVDDRAELLRYTNNIPLLGKLVNHQPLWSTNPKLKSFSLE
KISAPDQRRVQEALVVKDLLNVLIGLEGTYIRYFNDYEPSDPETPIEFKIAKKMDPSF
KTFSRRIVRYGKQYMILTRAYEKWSDTSFGMVLQRFAYEIRRFLEDVYLKTLVERLER
DFNKVPNFSIRELEQIINETEVNKQMELLYNIYEEIFREIEERRTNQSSQEDFNNFMD
SMKNESSLHLRLMVAFDTTVYPVPKGGAILKIFQQKILENLGDRSSVMFLKKLLNNIS
QDYCTMLYEWLTQGILNDPYQEFMTYDDLEGKTDNIFDTRDRAWDTQYFIRKDVLLRD
CDSEEDKNLLFKMLRTGILLKVVRASLQIPTIPSNSSDITIQEINDFADLMEGSNLEL
YVDKCYSRANEIFLKLFFQGYDLINVLKHLQQIFLGYQSGHNVLKFLTKNMGELTKHY
RNDNNANYDKLLQNFELERQSENPNNLMRQLLMIQFDTETLPQVLSHYLQIYPEVPEN
NSANDDSDPLMHANNFKNMNAILFDELSKERTGAYHGSNLELYTPKSAIYHLKFDINI
PYPLNIIISRTCMIKYQIILRYQLVLQYHSRLLDETWMDLNKTPSWKYRGYSHTVKRR
IVRATRVLHAKMNHFIKTIMEYFNQNVIDKEVYSLEKCYRNPTLAVAIQNELEGGLTN
IMTNRCLSDLIPLQLQIFDIVYKFCKFIKSMRAKLCQLDPVLYEKHKSGMMKTLNEGY
RTNNGGQEDVGYQEDAALELIQKLIEYISNASSIFRKCLINFTQELSTEKFDFYDSSS
VDAAGIERVLYSIVPPRSASASSQR"
gene complement(<450815..>451153)
/locus_tag="YHR173C"
/db_xref="GeneID:856578"
mRNA complement(<450815..>451153)
/locus_tag="YHR173C"
/product="uncharacterized protein"
/transcript_id="NM_001270738.1"
/db_xref="GeneID:856578"
CDS complement(450815..451153)
/locus_tag="YHR173C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/note="hypothetical protein; expressed at both mRNA and
protein levels; SWAT-GFP and mCherry fusion proteins
localize to the vacuole"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001257667.1"
/db_xref="GeneID:856578"
/db_xref="SGD:S000001216"
/translation="MLFFFIYAWCHLSHTILQPSTKIQMIVRSKPESNGEKKLMNHDE
PNDQYNQRNYPSEVYADLVSHFMNFAYGKVHESGLQKKGVRCVDVSISGCQFWNGIPL
SASWCTSLVT"
gene <451327..>452640
/gene="ENO2"
/locus_tag="YHR174W"
/db_xref="GeneID:856579"
mRNA <451327..>452640
/gene="ENO2"
/locus_tag="YHR174W"
/product="phosphopyruvate hydratase ENO2"
/transcript_id="NM_001179305.1"
/db_xref="GeneID:856579"
CDS 451327..452640
/gene="ENO2"
/locus_tag="YHR174W"
/EC_number="4.2.1.11"
/experiment="EXISTENCE:direct assay:GO:0000015
phosphopyruvate hydratase complex [PMID:6282834]"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:16565073]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16962558|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836]"
/experiment="EXISTENCE:direct assay:GO:0009898 cytoplasmic
side of plasma membrane [PMID:16544286]"
/experiment="EXISTENCE:direct assay:GO:0032889 regulation
of vacuole fusion, non-autophagic [PMID:16565073]"
/experiment="EXISTENCE:direct assay:GO:1904408 melatonin
binding [PMID:31708896]"
/experiment="EXISTENCE:mutant phenotype:GO:0004634
phosphopyruvate hydratase activity [PMID:6282834]"
/experiment="EXISTENCE:mutant phenotype:GO:0006096
glycolytic process [PMID:6282834]"
/experiment="EXISTENCE:mutant phenotype:GO:0032889
regulation of vacuole fusion, non-autophagic
[PMID:16565073]"
/note="Enolase II, a phosphopyruvate hydratase; catalyzes
conversion of 2-phosphoglycerate to phosphoenolpyruvate
during glycolysis and the reverse reaction during
gluconeogenesis; mRNA is asymmetrically distributed to
daughter cells in a Sir2p-dependent manner, and asymmetry
is lost during replicative aging; expression induced in
response to glucose; ENO2 has a paralog, ENO1, that arose
from the whole genome duplication"
/codon_start=1
/product="phosphopyruvate hydratase ENO2"
/protein_id="NP_012044.1"
/db_xref="GeneID:856579"
/db_xref="SGD:S000001217"
/translation="MAVSKVYARSVYDSRGNPTVEVELTTEKGVFRSIVPSGASTGVH
EALEMRDEDKSKWMGKGVMNAVNNVNNVIAAAFVKANLDVKDQKAVDDFLLSLDGTAN
KSKLGANAILGVSMAAARAAAAEKNVPLYQHLADLSKSKTSPYVLPVPFLNVLNGGSH
AGGALALQEFMIAPTGAKTFAEAMRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPN
IQTAEEALDLIVDAIKAAGHDGKVKIGLDCASSEFFKDGKYDLDFKNPESDKSKWLTG
VELADMYHSLMKRYPIVSIEDPFAEDDWEAWSHFFKTAGIQIVADDLTVTNPARIATA
IEKKAADALLLKVNQIGTLSESIKAAQDSFAANWGVMVSHRSGETEDTFIADLVVGLR
TGQIKTGAPARSERLAKLNQLLRIEEELGDKAVYAGENFHHGDKL"
gene <452872..>453441
/gene="CTR2"
/locus_tag="YHR175W"
/db_xref="GeneID:856580"
mRNA <452872..>453441
/gene="CTR2"
/locus_tag="YHR175W"
/product="low-affinity Cu transporter"
/transcript_id="NM_001179306.3"
/db_xref="GeneID:856580"
CDS 452872..453441
/gene="CTR2"
/locus_tag="YHR175W"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:11523804]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0005375
copper ion transmembrane transporter activity
[PMID:7499355]"
/experiment="EXISTENCE:genetic interaction:GO:0015677
copper ion import [PMID:7499355]"
/experiment="EXISTENCE:mutant phenotype:GO:0005375 copper
ion transmembrane transporter activity [PMID:11523804]"
/experiment="EXISTENCE:mutant phenotype:GO:0006825 copper
ion transport [PMID:11523804]"
/experiment="EXISTENCE:mutant phenotype:GO:0006878
intracellular copper ion homeostasis [PMID:23450501]"
/note="Low-affinity copper transporter of the vacuolar
membrane; mutation confers resistance to toxic copper
concentrations, while overexpression confers resistance to
copper starvation; regulated by nonsense-mediated mRNA
decay pathway"
/codon_start=1
/product="low-affinity Cu transporter"
/protein_id="NP_012045.3"
/db_xref="GeneID:856580"
/db_xref="SGD:S000001218"
/translation="MDDKKTWSTVTLRTFNQLVTSSLIGYSKKMDSMNHKMEGNAGHD
HSDMHMGDGDDTCSMNMLFSWSYKNTCVVFEWWHIKTLPGLILSCLAIFGLAYLYEYL
KYCVHKRQLSQRVLLPNRSLTKINQADKVSNSILYGLQVGFSFMLMLVFMTYNGWLML
AVVCGAIWGNYSWCTSYSPEIDDSSLACH"
gene <453558..>453707
/locus_tag="YHR175W-A"
/db_xref="GeneID:1466530"
mRNA <453558..>453707
/locus_tag="YHR175W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184554.1"
/db_xref="GeneID:1466530"
CDS 453558..453707
/locus_tag="YHR175W-A"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:40704575]"
/note="hypothetical protein; identified by fungal homology
and RT-PCR"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878090.1"
/db_xref="GeneID:1466530"
/db_xref="SGD:S000028553"
/translation="MINYVNITCIIFSTRTLLVFDTSLYIPPFMLSFIGYSLSNQNSP
LFLYH"
gene <454229..>455527
/gene="FMO1"
/locus_tag="YHR176W"
/db_xref="GeneID:856581"
mRNA <454229..>455527
/gene="FMO1"
/locus_tag="YHR176W"
/product="N,N-dimethylaniline monooxygenase"
/transcript_id="NM_001179307.3"
/db_xref="GeneID:856581"
CDS 454229..455527
/gene="FMO1"
/locus_tag="YHR176W"
/experiment="EXISTENCE:direct assay:GO:0004499
N,N-dimethylaniline monooxygenase activity
[PMID:10600176]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:10077572]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0006457 protein
folding [PMID:10077572]"
/note="Flavin-containing monooxygenase; localized to the
cytoplasmic face of the ER membrane; catalyzes oxidation
of biological thiols to maintain the ER redox buffer ratio
for correct folding of disulfide-bonded proteins"
/codon_start=1
/product="N,N-dimethylaniline monooxygenase"
/protein_id="NP_012046.4"
/db_xref="GeneID:856581"
/db_xref="SGD:S000001219"
/translation="MTVNDKKRLAIIGGGPGGLAAARVFSQSLPNFEIEIFVKDYDIG
GVWHYPEQKSDGRVMYDHLETNISKKLMQFSGFPFEENVPLYPSRRNIWEYLKAYYKT
FIANKDAISIHFSTEVTYLKKKNSQWEITSKDELRTTKSDFDFVIVASGHYSVPKLPT
NIAGLDLWFDNKGAFHSKDFKNCEFAREKVVIVVGNGSSGQDIANQLTTVAKKVYNSI
KEPASNQLKAKLIETVQTIDSADWKNRSVTLSDGRVLQNIDYIIFATGYYYSFPFIEP
SVRLEVLGEGVTGDKHSSVNLHNLWEHMIYVKDPTLSFILTPQLVIPFPLSELQAAIM
VEVFCKSLPITTTFDSNACGTHNFPKGKDLEYYAELQELLNSIPRRVGHFEPVVWDDR
LIDLRNSSYTDKEERNVLLAEHAQALKKKKAPYFLPAPHT"
gene <456591..>457952
/gene="ROF1"
/locus_tag="YHR177W"
/db_xref="GeneID:856582"
mRNA <456591..>457952
/gene="ROF1"
/locus_tag="YHR177W"
/product="Rof1p"
/transcript_id="NM_001179308.1"
/db_xref="GeneID:856582"
CDS 456591..457952
/gene="ROF1"
/locus_tag="YHR177W"
/experiment="EXISTENCE:curator inference:GO:0005634
nucleus [PMID:24994900]"
/experiment="EXISTENCE:direct assay:GO:0003677 DNA binding
[PMID:24994900]"
/note="Putative transcription factor containing a WOPR
domain; binds DNA in vitro; similar to C. albicans Wor1p
transcription factor that regulates white-opaque
switching; overexpression causes a cell cycle delay or
arrest"
/codon_start=1
/product="Rof1p"
/protein_id="NP_012047.1"
/db_xref="GeneID:856582"
/db_xref="SGD:S000001220"
/translation="MMDISPTCYGYIDDEQDLALVFQGVFNGNLRCIERRPYDAEKVE
LVNPGNIFVFNEEKSGIKRWTDGFSWSPSRISGKFLVYREYNRLGSTHDLPLHNVPEY
NIFERAHRKYFYTGLLKKTFSLKFNMDPTDSTKLETFHLIAYYTEKDIHQGSLRRPSE
NPFFHKFRPSQKLLDALQKVAVGNGRSNPSKNNERGRTKAHNYKTRRSLSSSPSYCDL
LSNYNNHPGNIPVRTAVQLPLTTFNNAPREMHQQQHRQQQQYLLPIDEQNKLPLPYMQ
HQPQPIGVYNPNYQPGLRRTVSQPMIFCNTYNTLPQQPTAAPYERRGVSPSVIYSSNT
LSPIPYQNIDPYSSRSGPECNHSKAPIAPTMMPPVHHILVHDYRQPKPVTDSINPPNV
NITTSTTNKNLDGIYILPAPRMNPPAQTQYQMIHAPDSMQHPPTFSKNNTSSNPKSHQ
YSK"
gene <459299..>461530
/gene="STB5"
/locus_tag="YHR178W"
/db_xref="GeneID:856583"
mRNA <459299..>461530
/gene="STB5"
/locus_tag="YHR178W"
/product="Stb5p"
/transcript_id="NM_001179309.3"
/db_xref="GeneID:856583"
CDS 459299..461530
/gene="STB5"
/locus_tag="YHR178W"
/experiment="EXISTENCE:curator inference:GO:0005634
nucleus [PMID:11943786]"
/experiment="EXISTENCE:direct assay:GO:0003700 DNA-binding
transcription factor activity [PMID:11943786]"
/experiment="EXISTENCE:direct assay:GO:0006368
transcription elongation by RNA polymerase II
[PMID:11943786]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:19111667]"
/experiment="EXISTENCE:mutant phenotype:GO:0003700
DNA-binding transcription factor activity [PMID:11943786]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:16914749]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress [PMID:16914749]"
/note="Transcription factor; regulates multidrug
resistance and the oxidative stress response; forms a
heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger
domain that interacts with a pleiotropic drug resistance
element in vitro; negative regulator of autophagy,
modulating the transcription of genes involved in NADPH
production and the pentose phosphate pathway; Stb5p
expression is reduced during nitrogen starvation and
autophagy induction, while STB5 mRNA levels are
significantly increased"
/codon_start=1
/product="Stb5p"
/protein_id="NP_012048.3"
/db_xref="GeneID:856583"
/db_xref="SGD:S000001221"
/translation="MDGPNFAHQGGRSQRTTELYSCARCRKLKKKCGKQIPTCANCDK
NGAHCSYPGRAPRRTKKELADAMLRGEYVPVKRNKKVGKSPLSTKSMPNSSSPLSANG
AITPGFSPYENDDAHKMKQLKPSDPINLVMGASPNSSEGVSSLISVLTSLNDNSNPSS
HLSSNENSMIPSRSLPASVQQSSTTSSFGGYNTPSPLISSHVPANAQAVPLQNNNRNT
SNGDNGSNVNHDNNNGSTNTPQLSLTPYANNSAPNGKFDSVPVDASSIEFETMSCCFK
GGRTTSWVREDGSFKSIDRSLLDRFIAAYFKHNHRLFPMIDKIAFLNDAATITDFERL
YDNKNYPDSFVFKVYMIMAIGCTTLQRAGMVSQDEECLSEHLAFLAMKKFRSVIILQD
IETVRCLLLLGIYSFFEPKGSSSWTISGIIMRLTIGLGLNRELTAKKLKSMSALEAEA
RYRVFWSAYCFERLVCTSLGRISGIDDEDITVPLPRALYVDERDDLEMTKLMISLRKM
GGRIYKQVHSVSAGRQKLTIEQKQEIISGLRKELDEIYSRESERRKLKKSQMDQVERE
NNSTTNVISFHSSEIWLAMRYSQLQILLYRPSALMPKPPIDSLSTLGEFCLQAWKHTY
TLYKKRLLPLNWITLFRTLTICNTILYCLCQWSIDLIESKIEIQQCVEILRHFGERWI
FAMRCADVFQNISNTILDISLSHGKVPNMDQLTRELFGASDSYQDILDENNVDVSWVD
KLV"
gene <462502..>463704
/gene="OYE2"
/locus_tag="YHR179W"
/db_xref="GeneID:856584"
mRNA <462502..>463704
/gene="OYE2"
/locus_tag="YHR179W"
/product="NADPH dehydrogenase"
/transcript_id="NM_001179310.1"
/db_xref="GeneID:856584"
CDS 462502..463704
/gene="OYE2"
/locus_tag="YHR179W"
/EC_number="1.6.99.1"
/experiment="EXISTENCE:direct assay:GO:0003959 NADPH
dehydrogenase activity [PMID:8454584]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:mutant phenotype:GO:0006915
apoptotic process [PMID:17897954]"
/note="Conserved NADPH oxidoreductase containing flavin
mononucleotide (FMN); responsible for geraniol reduction
into citronellol during fermentation; homologous to Oye3p
with different ligand binding and catalytic properties;
may be involved in sterol metabolism, oxidative stress
response, and programmed cell death; protein abundance
increases in response to DNA replication stress"
/codon_start=1
/product="NADPH dehydrogenase"
/protein_id="NP_012049.1"
/db_xref="GeneID:856584"
/db_xref="SGD:S000001222"
/translation="MPFVKDFKPQALGDTNLFKPIKIGNNELLHRAVIPPLTRMRAQH
PGNIPNRDWAVEYYAQRAQRPGTLIITEGTFPSPQSGGYDNAPGIWSEEQIKEWTKIF
KAIHENKSFAWVQLWVLGWAAFPDTLARDGLRYDSASDNVYMNAEQEEKAKKANNPQH
SITKDEIKQYVKEYVQAAKNSIAAGADGVEIHSANGYLLNQFLDPHSNNRTDEYGGSI
ENRARFTLEVVDAVVDAIGPEKVGLRLSPYGVFNSMSGGAETGIVAQYAYVLGELERR
AKAGKRLAFVHLVEPRVTNPFLTEGEGEYNGGSNKFAYSIWKGPIIRAGNFALHPEVV
REEVKDPRTLIGYGRFFISNPDLVDRLEKGLPLNKYDRDTFYKMSAEGYIDYPTYEEA
LKLGWDKN"
repeat_region complement(463920..464147)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006999"
gene <465178..>465669
/locus_tag="YHR180W"
/db_xref="GeneID:856585"
mRNA <465178..>465669
/locus_tag="YHR180W"
/product="uncharacterized protein"
/transcript_id="NM_001348842.1"
/db_xref="GeneID:856585"
CDS 465178..465669
/locus_tag="YHR180W"
/note="hypothetical protein; conserved among S. cerevisiae
strains"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001335743.1"
/db_xref="GeneID:856585"
/db_xref="SGD:S000001223"
/translation="MEMHWITLVAFIATFFNLAATSINNSSLPDVDLTNPLRFFTNIP
AGLNFNEVIFLERNGFYLGGIDSPSIYHLINGTAVYFGDVRDNIMPGTVGTTRNVTDV
DYGSLLTEYGYEANTDYVSRWIATHVVISPLNATEFFQTPVPVPVPVPITILHQQVNS
KLH"
repeat_region 466549..466863
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007009"
repeat_region complement(466884..466975)
/note="Ty3 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007004"
gene 466990..467061
/locus_tag="YNCH0015W"
/db_xref="GeneID:856586"
tRNA 466990..467061
/locus_tag="YNCH0015W"
/product="tRNA-Thr"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:856586"
/db_xref="SGD:S000006751"
gene <467228..>467914
/gene="SVP26"
/locus_tag="YHR181W"
/gene_synonym="ERV26"
/db_xref="GeneID:856587"
mRNA <467228..>467914
/gene="SVP26"
/locus_tag="YHR181W"
/gene_synonym="ERV26"
/product="Svp26p"
/transcript_id="NM_001179312.3"
/db_xref="GeneID:856587"
CDS 467228..467914
/gene="SVP26"
/locus_tag="YHR181W"
/gene_synonym="ERV26"
/experiment="EXISTENCE:direct assay:GO:0000139 Golgi
membrane [PMID:16107716]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:16957051]"
/experiment="EXISTENCE:direct assay:GO:0006888 endoplasmic
reticulum to Golgi vesicle-mediated transport
[PMID:16957051]"
/experiment="EXISTENCE:direct assay:GO:0030134
COPII-coated ER to Golgi transport vesicle
[PMID:16957051]"
/experiment="EXISTENCE:mutant phenotype:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:30842360|PMID:16957051]"
/experiment="EXISTENCE:mutant phenotype:GO:0031505
fungal-type cell wall organization [PMID:16107716]"
/experiment="EXISTENCE:mutant phenotype:GO:0045053 protein
retention in Golgi apparatus [PMID:16107716]"
/experiment="EXISTENCE:mutant phenotype:GO:0090110
COPII-coated vesicle cargo loading [PMID:30842360]"
/experiment="EXISTENCE:mutant phenotype:GO:0097020 COPII
receptor activity [PMID:20236934]"
/experiment="EXISTENCE:physical interaction:GO:0097020
COPII receptor activity [PMID:20236934]"
/note="ER exit adaptor for Golgi mannosyltransferases;
facilitates the exit of Kre2p, Ktr1p and Mnn4p from the
ER; involved in COPII-coated vesicle cargo loading and
transport; may also promote the retention of proteins in
the early Golgi compartment; integral membrane protein of
early Golgi and ER"
/codon_start=1
/product="Svp26p"
/protein_id="NP_012051.3"
/db_xref="GeneID:856587"
/db_xref="SGD:S000001224"
/translation="MLLELISYAGTVSGFLFLTLSIASGLYYISELVEEHTEPTRRFL
TRAIYGIILILILLLLLDGFPFKLTLFSIACYIVYYQNLKSFPFISLTSPTFLLSCVC
VVLNHYFWFKYFNDTEVPPQFKFDPNYIPRRRASFAEVASFFGICVWFIPFALFVSLS
AGDYVLPTTSEQHMAKKNDDITTNNQPKFRKRAVGLARVVINSVRKYIYSLARVFGYE
IEPDFDRLAV"
gene <468219..>470576
/gene="RGD3"
/locus_tag="YHR182W"
/db_xref="GeneID:856588"
mRNA <468219..>470576
/gene="RGD3"
/locus_tag="YHR182W"
/product="Rgd3p"
/transcript_id="NM_001179313.1"
/db_xref="GeneID:856588"
CDS 468219..470576
/gene="RGD3"
/locus_tag="YHR182W"
/experiment="EXISTENCE:direct assay:GO:0005096 GTPase
activator activity [PMID:32941095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:32941095]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0030659 cytoplasmic
vesicle membrane [PMID:32941095]"
/experiment="EXISTENCE:direct assay:GO:0043547 positive
regulation of GTPase activity [PMID:32941095]"
/experiment="EXISTENCE:mutant phenotype:GO:0007163
establishment or maintenance of cell polarity
[PMID:32941095]"
/note="GTPase activating protein (GAP) for Rho3p, involved
in cell polarity green fluorescent protein (GFP)-fusion
protein localizes to the cell periphery and cytoplasm;
relocalizes from bud neck to cytoplasm upon DNA
replication stress"
/codon_start=1
/product="Rgd3p"
/protein_id="NP_012052.1"
/db_xref="GeneID:856588"
/db_xref="SGD:S000001225"
/translation="MSVKEHNEEDIIGDELQNSRQLSIDCDSVKISLRNTYWTKDYTT
GIKLFIKHMKRENDLLIKDIKFYNDFVNKFWKPTLNNLQKMEATNSMNSRLLEVMSKQ
FNIISTEQVERDCKIPLQELRDLNESFLREAENDLSSRYSAYIKDLVAAKEALIGCEK
RVQSIYKLKKANTPVENSSSVFDNGKDSAPLTRLNFVCEFPYTLDERLKFEDCDQFMS
FLQTLKGKVILEKSVFSVPGLSNQSFQGRSLIKELKKLEPRLNLSLFNIDRIGNEFIQ
LGIIQEYSLSFYSSKVSQFDQEKYYYWNSEVLATQESNGNAGNRKKKSYGELTHSDNE
HEEKSNVSSIKTSISDWIRKVSQHDNDDCDAAGSTDMNKNEWKSLKQQLESSQDIFFS
KCCQLEYSKVQLEKTIYDYCKNYSKMEDGIKRALESSNMMFQQKCEKFTDSPVCSLQE
AQLPQETANADVRGFFLRDNGIPFRRWNILEASDPVDACKEISIKSEKFFCGSEINNE
LAALDTLGAIKIILRQIEKEPNANKVIQSWHRDIDFVRVSNLKRDLLGEFKGSKTTEN
TNSIITAHFFENSHSYVTNDLVGLIKLWLLELPDSLIPSNHYDDLIKAEKSLTSLCEQ
FPTSSLRFLQELANHFQLINSKYSLPPQTIQDLFRDNSDIDIPLAHHFVRRTGLQNPI
DIKILSPTLSTFFINERTVETLQTLIANRITTATTATLTEPPTIIIKDTTAPIHSTPK
PPPNDKDGHFIPRPFKTSSTPTTPERPKRKSGLFLPINVNDVPST"
gene <470960..>472429
/gene="GND1"
/locus_tag="YHR183W"
/db_xref="GeneID:856589"
mRNA <470960..>472429
/gene="GND1"
/locus_tag="YHR183W"
/product="phosphogluconate dehydrogenase (decarboxylating)
GND1"
/transcript_id="NM_001179314.3"
/db_xref="GeneID:856589"
CDS 470960..472429
/gene="GND1"
/locus_tag="YHR183W"
/EC_number="1.1.1.44"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278|PMID:24769239]"
/experiment="EXISTENCE:mutant phenotype:GO:0004616
phosphogluconate dehydrogenase (decarboxylating) activity
[PMID:1328471]"
/experiment="EXISTENCE:mutant phenotype:GO:0009051
pentose-phosphate shunt, oxidative branch [PMID:1328471]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress [PMID:9480895]"
/note="6-phosphogluconate dehydrogenase (decarboxylating);
catalyzes an NADPH regenerating reaction in the pentose
phosphate pathway; required for growth on
D-glucono-delta-lactone and adaptation to oxidative
stress; GND1 has a paralog, GND2, that arose from the
whole genome duplication"
/codon_start=1
/product="phosphogluconate dehydrogenase (decarboxylating)
GND1"
/protein_id="NP_012053.3"
/db_xref="GeneID:856589"
/db_xref="SGD:S000001226"
/translation="MSADFGLIGLAVMGQNLILNAADHGFTVCAYNRTQSKVDHFLAN
EAKGKSIIGATSIEDFISKLKRPRKVMLLVKAGAPVDALINQIVPLLEKGDIIIDGGN
SHFPDSNRRYEELKKKGILFVGSGVSGGEEGARYGPSLMPGGSEEAWPHIKNIFQSIS
AKSDGEPCCEWVGPAGAGHYVKMVHNGIEYGDMQLICEAYDIMKRLGGFTDKEISDVF
AKWNNGVLDSFLVEITRDILKFDDVDGKPLVEKIMDTAGQKGTGKWTAINALDLGMPV
TLIGEAVFARCLSALKNERIRASKVLPGPEVPKDAVKDREQFVDDLEQALYASKIISY
AQGFMLIREAAATYGWKLNNPAIALMWRGGCIIRSVFLGQITKAYREEPDLENLLFNK
FFADAVTKAQSGWRKSIALATTYGIPTPAFSTALSFYDGYRSERLPANLLQAQRDYFG
AHTFRVLPECASDNLPVDKDIHINWTGHGGNVSSSTYQA"
gene <472744..>474459
/gene="SSP1"
/locus_tag="YHR184W"
/gene_synonym="SPO3"
/db_xref="GeneID:856590"
mRNA <472744..>474459
/gene="SSP1"
/locus_tag="YHR184W"
/gene_synonym="SPO3"
/product="Ssp1p"
/transcript_id="NM_001179315.1"
/db_xref="GeneID:856590"
CDS 472744..474459
/gene="SSP1"
/locus_tag="YHR184W"
/gene_synonym="SPO3"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:11742972]"
/experiment="EXISTENCE:direct assay:GO:0070056 prospore
membrane leading edge [PMID:11742972|PMID:24390141]"
/experiment="EXISTENCE:mutant phenotype:GO:0030476
ascospore wall assembly [PMID:11742972]"
/experiment="EXISTENCE:mutant phenotype:GO:0032120
ascospore-type prospore membrane formation
[PMID:11742972]"
/note="BAR domain protein involved in the control of
meiotic nuclear division; coordinates meiosis with spore
formation; subunit of the leading edge protein (LEP)
complex (Ssp1-Ady3-Don1-Irc10) that forms a ring-like
structure at the leading edge of the prospore membrane
during meiosis II; BAR domain required for recruitment to
the leading edge of the membrane where multimerization
leads to LEP complex assembly; required for assembly of
the leading edge coat, prospore membrane shaping and
organization"
/codon_start=1
/product="Ssp1p"
/protein_id="NP_012054.1"
/db_xref="GeneID:856590"
/db_xref="SGD:S000001227"
/translation="MRSSGTYENDPSGEITSTSPKQSKQKKPTKFRERMRRWLQNGKN
NNHQGEEDVPEIFNKNFYPQTGMTAFNNNDNGEVQDVTNNFFLPSEDESGPVQSSVKT
FLTGNNDEDSNFQQNQNPKQKSELPKSPYRQKPTQEIALLKDLFVTNKYDDPYLNSST
RFGNITSTFPSSLSLRTVTLQTIKKRIDCISAKKKEVWKTEEKFLKDILMWLQSSNFE
DPDTISLIHEIEKIFEEDIHFEQNVSDCLKEISNNFEYICMRETQLINEGNILKNDLK
KYAKAREHKGEKHEDTEVLREKVISSQKSFDVTKRHYKHAISITTRQLFMNLAFEYYE
NCSDMKDISRKYLQESLSTLQTIDTLSFSEELEKIRKRRFDKFWAKTNPDPTNNIQKF
VNMRTGVAGFNDSLMNHLYGKLSFGVAPVEEELQNSQPEHTDVPENVWNEVLSDYNSM
DGNPITSNKFLSAKELEPDQLVELLAQEKEEKEAKNISSSTAEVPSISQPEIKKENLE
SNDSLILRSTKRNVNVNAASLRNLSIKKTQVKPESASEESKVLAAALNDAKQNLDENV
WRTPI"
gene complement(<474627..>475340)
/gene="PFS1"
/locus_tag="YHR185C"
/gene_synonym="ADY1"
/db_xref="GeneID:856591"
mRNA complement(<474627..>475340)
/gene="PFS1"
/locus_tag="YHR185C"
/gene_synonym="ADY1"
/product="Pfs1p"
/transcript_id="NM_001179316.3"
/db_xref="GeneID:856591"
CDS complement(474627..475340)
/gene="PFS1"
/locus_tag="YHR185C"
/gene_synonym="ADY1"
/experiment="EXISTENCE:mutant phenotype:GO:0030476
ascospore wall assembly [PMID:11553705]"
/note="Sporulation protein required for prospore membrane
formation; required for prospore membrane formation at
selected spindle poles; ensures functionality of all four
spindle pole bodies during meiosis II; not required for
meiotic recombination or meiotic chromosome segregation"
/codon_start=1
/product="Pfs1p"
/protein_id="NP_012055.3"
/db_xref="GeneID:856591"
/db_xref="SGD:S000001228"
/translation="MNQGYTQLSAPELKETKTSKLNKMNNFRSSPIAEIINKIPPDCG
KIQNTTFPEFNPALRRRQHEQWPAYEKPIRVTDSMSPQLSSINCLPNLYPHGTLPLPN
PYLSYLNHIEKVNCQDVKFSNWSVLHNSNNGFEIPTYFSPRTTQNMPCSEKVESWLER
LPIFVGFDGYLFTNCFDYEYMLDWEETEFTFEKTSCMETDYSKALTDTDIIYIQEKKI
EALIRNQYLKEYEFSQKDF"
gene complement(475706..475778)
/locus_tag="YNCH0016C"
/db_xref="GeneID:856592"
tRNA complement(475706..475778)
/locus_tag="YNCH0016C"
/product="tRNA-Val"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Valine tRNA (tRNA-Val), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:856592"
/db_xref="SGD:S000006768"
gene complement(<475999..>480672)
/gene="KOG1"
/locus_tag="YHR186C"
/gene_synonym="LAS24"
/db_xref="GeneID:856593"
mRNA complement(<475999..>480672)
/gene="KOG1"
/locus_tag="YHR186C"
/gene_synonym="LAS24"
/product="ubiquitin-binding TORC1 subunit KOG1"
/transcript_id="NM_001179317.1"
/db_xref="GeneID:856593"
CDS complement(475999..480672)
/gene="KOG1"
/locus_tag="YHR186C"
/gene_synonym="LAS24"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane
[PMID:16394584|PMID:14562095|PMID:17560372|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16394584]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405]"
/experiment="EXISTENCE:direct assay:GO:0043130 ubiquitin
binding [PMID:21070969]"
/experiment="EXISTENCE:mutant phenotype:GO:0009267
cellular response to starvation [PMID:12408816]"
/experiment="EXISTENCE:physical interaction:GO:0001558
regulation of cell growth [PMID:12408816]"
/experiment="EXISTENCE:physical interaction:GO:0031931
TORC1 complex [PMID:12408816]"
/note="Ubiquitin-binding protein; centrally regulates
metabolic homeostasis and carbon utilization during
nutrient limitation by managing Snf1p activity; subunit of
TORC1 rapamycin-sensitive complex involved in growth
control; central scaffold that holds the different
components of TORC1 together; contains four HEAT repeats
and seven WD-40 repeats"
/codon_start=1
/product="ubiquitin-binding TORC1 subunit KOG1"
/protein_id="NP_012056.1"
/db_xref="GeneID:856593"
/db_xref="SGD:S000001229"
/translation="MPEIYGPQPLKPLNTVMRHGFEEQYQSDQLLQSLANDFIFYFDD
KRHKTNGNPIPEEDKQRDVNRYYQPITDWKIMKDRQKTVSAALLLCLNLGVDPPDVMK
THPCARVEAWVDPLNFQDSKKAIEQIGKNLQAQYETLSLRTRYKQSLDPCVEDVKRFC
NSLRRTSKEDRILFHYNGHGVPKPTKSGEIWVFNRGYTQYIPVSLYDLQTWLGAPCIF
VYDCNSAENILINFQKFVQKRIKDDEEGNHDVAAPSPTSAYQDCFQLASCTSDELLLM
SPELPADLFSCCLTCPIEISIRIFLMQSPLKDSKYKIFFENSTSNQPFGDSKNSFKSK
IPNVNIPGMLSDRRTPLGELNWIFTAITDTIAWTSLPRPLFKKLFRHDLMIAALFRNF
LLAKRIMPWYNCHPVSDPELPDSITTHPMWKSWDLAMDEVLTKIVIDLKNAPPATALE
SQMILQQQETLQNGGSSKSNAQDTKAGSIQTQSRFAVANLSTMSLVNNPALQSRKSIS
LQSSQQQLQQQQQQQQQFTGFFEQNLTAFELWLKYASNVRHPPEQLPIVLQVLLSQVH
RIRALVLLSRFLDLGPWAVYLSLSIGIFPYVLKLLQSPAPELKPILVFIWARIMSIDY
KNTQSELIKEKGYMYFVTVLVPDWGVNGMSATNGSAMINSGNPLTMTASQNINGPSSR
YYERQQGNRTSNLGHNNLPFYHSNDTTDEQKAMAVFVLASFVRNFPLGQKNCFSLELV
NKLCFYIDNSEIPLLRQWCVILLGLLFADNPLNRFVCMNTGAVEILLKSLKDPVPEVR
TASIFALKHFISGFQDAEVILRLQQEFEEQYQQLHSQLQHLQNQSHLQQQQSQQQQQH
LEQQQMKIEKQIRHCQVMQNQLEVIDLRKLKRQEIGNLISILPLINDGSSLVRKELVV
YFSHIVSRYSNFFIVVVFNDLLEEIKLLEKSDINTRNTSDKYSVSQGSIFYTVWKSLL
ILAEDPFLENKELSKQVIDYILLELSAHKELGGPFAVMEKFLLKRSSKAHQTGKFGFN
SSQVQFVKSSLRSFSPNERVDNNAFKKEQQQHDPKISHPMRTSLAKLFQSLGFSESNS
DSDTQSSNTSMKSHTSKKGPSGLYLLNGNNNIYPTAETPRFRKHTEPLQLPLNSSFLD
YSREYFQEPQMKKQEADEPGSVEYNARLWRRNRNETIIQETQGEKKLSIYGNWSKKLI
SLNNKSQPKLMKFAQFEDQLITADDRSTITVFDWEKGKTLSKFSNGTPFGTKVTDLKL
INEDDSALLLTGSSDGVIKIYRDYQDVDTFKIVSAWRGLTDMLLTPRSTGLLTEWLQI
RGSLLTTGDVKVIRVWDAHTETVEVDIPAKTSSLITSLTADQLAGNIFVAGFADGSLR
VYDRRLDPRDSMIRRWRAGNDKQGVWINNVHLQRGGYRELVSGATNGVVELWDIRSED
PVESFVDQNVTSQYGSQQKPTTMTCMQVHEHAPIIATGTKQIKIWTTSGDLLNSFKNS
HNNGVTSTLAATGIPKSLSYSSTSDAFLSSMAFHPHRMMIAATNSHDSIVNIYKCEDE
RIDYF"
gene <480990..>481919
/gene="IKI1"
/locus_tag="YHR187W"
/gene_synonym="ELP5; HAP2; TOT5"
/db_xref="GeneID:856594"
mRNA <480990..>481919
/gene="IKI1"
/locus_tag="YHR187W"
/gene_synonym="ELP5; HAP2; TOT5"
/product="Elongator subunit IKI1"
/transcript_id="NM_001179318.3"
/db_xref="GeneID:856594"
CDS 480990..481919
/gene="IKI1"
/locus_tag="YHR187W"
/gene_synonym="ELP5; HAP2; TOT5"
/experiment="EXISTENCE:direct assay:GO:0000049 tRNA
binding [PMID:22343726]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12139626]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:12139626]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:36164978]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:22343726]"
/experiment="EXISTENCE:direct assay:GO:0033588 elongator
holoenzyme complex [PMID:11689709]"
/experiment="EXISTENCE:mutant phenotype:GO:0006400 tRNA
modification [PMID:15769872]"
/experiment="EXISTENCE:mutant phenotype:GO:0016887 ATP
hydrolysis activity [PMID:22343726]"
/note="Subunit of hexameric RecA-like ATPase Elp456
Elongator subcomplex; which is required for modification
of wobble nucleosides in tRNA; iki1 mutations confer
resistance to the K. lactis toxin zymocin; localizes to
cytosol, nucleus and peroxisomes"
/codon_start=1
/product="Elongator subunit IKI1"
/protein_id="NP_012057.3"
/db_xref="GeneID:856594"
/db_xref="SGD:S000001230"
/translation="MASSSHNPVILLKRILSLTESSPFILCLDSIAQTSYKLIQEFVH
QSKSKGNEYPIVYISFETVNKPSYCTQFIDATQMDFVHLVKQIISYLPAATATQAKKH
MVIIDSLNYISTEYITRFLSEIASPHCTMVATYHKDIKDENRTVIPDWNNNYPDKLTL
LQFMATTIVDIDVVLTGTLDTEEVSELLNEFRIPRGLNNDIFQLRLVNKRKSGRSLEY
DFIVNSNTHEYELLSTTKQEEESSSNGLETPEMLQGLTTFNLGTSNKQKLAKDQVALP
FLEAQSFGQGGAIVYEYEKDDDYDEEDPYEDPF"
gene complement(<482005..>483837)
/gene="GPI16"
/locus_tag="YHR188C"
/db_xref="GeneID:856595"
mRNA complement(<482005..>483837)
/gene="GPI16"
/locus_tag="YHR188C"
/product="GPI-anchor transamidase subunit GPI16"
/transcript_id="NM_001179319.1"
/db_xref="GeneID:856595"
CDS complement(482005..483837)
/gene="GPI16"
/locus_tag="YHR188C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:11598210]"
/experiment="EXISTENCE:direct assay:GO:0042765 GPI-anchor
transamidase complex [PMID:11598210]"
/experiment="EXISTENCE:mutant phenotype:GO:0016255
attachment of GPI anchor to protein [PMID:11598210]"
/note="Subunit of the glycosylphosphatidylinositol
transamidase complex; involved in the addition of GPIs to
newly synthesized proteins; luminally-oriented, type I
integral membrane glycoprotein of the ER; human PIG-T
homolog"
/codon_start=1
/product="GPI-anchor transamidase subunit GPI16"
/protein_id="NP_012058.1"
/db_xref="GeneID:856595"
/db_xref="SGD:S000001231"
/translation="MILTLAYFMLGTLLLGVFAEDTVSQIGINDSLWYPYDEALVLKP
LPNNDLLLSFAFQLQSEPFDPAVSSMSYDAYEHYTTFPRAIPPLLESTATRQFHLRFT
RGFWDALSWGQLPHAGKEAGASGVELWSQVQAMDQEQAFHNWKKLSNSLSGLFCSSLN
FIDESRTTFPRRSYASDIGAPLFNSTEKLYLMRASLPNEPICTENLTPFIKLLPTRGK
SGLTSLLDGHKLFDSLWNSISLDIATICSEDEDALCHYEMDARIEMVTHVPSALARGE
RPIPKPLDGNTLRCDTDKPFDSYQCFPLPEPSQTHFKLSQLFARPINNGNLFANRPTR
ICAEVDRSTWTAFLSVDDTIFSTHDNCFDLSNDQNEGGSGYDFILESTDTTKVTPIVP
VPIHVSRSLTGNGQDRGGMRIVFHNDNDTPVKLIYFESLPWFMRVYLSSLQITSTTSP
QLQENDIILDKYYLQAADRKRPGHLEFTMLIPANTDIVMTYQFDKALLQFAEYPPDAN
HGFEIDAAVITVLSLESSSSLYEMRTSTLLLSLSTPDFSMPYNVIILTSTIMGLIFGM
LYNLMVKRMVTVEEADKITLQSGLKYKLLKLKEKFLGKKKTKTD"
gene <484028..>484600
/gene="PTH1"
/locus_tag="YHR189W"
/db_xref="GeneID:856596"
mRNA <484028..>484600
/gene="PTH1"
/locus_tag="YHR189W"
/product="aminoacyl-tRNA hydrolase"
/transcript_id="NM_001179320.3"
/db_xref="GeneID:856596"
CDS 484028..484600
/gene="PTH1"
/locus_tag="YHR189W"
/EC_number="3.1.1.29"
/experiment="EXISTENCE:direct assay:GO:0004045
peptidyl-tRNA hydrolase activity [PMID:12799450]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095|PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:genetic interaction:GO:0004045
peptidyl-tRNA hydrolase activity [PMID:12475929]"
/experiment="EXISTENCE:mutant phenotype:GO:0032543
mitochondrial translation [PMID:12799450]"
/note="One of two mitochondrially-localized peptidyl-tRNA
hydrolases; dispensable for respiratory growth on rich
medium, but required for respiratory growth on minimal
medium; see also PTH2"
/codon_start=1
/product="aminoacyl-tRNA hydrolase"
/protein_id="NP_012059.3"
/db_xref="GeneID:856596"
/db_xref="SGD:S000001232"
/translation="MSGKWRLVLTGIGNPEPQYAGTRHNVGLYMLELLRKRLGLQGRT
YSPVPNTGGKVHYIEDEHCTILRSDGQYMNLSGEQVCKVWARYAKYQARHVVIHDELS
VACGKVQLRAPSTSIRGHNGLRSLLKCSGGRVPFAKLAIGIGREPGSRSRDPASVSRW
VLGALTPQELQTLLTQSEPAAWRALTQYIS"
gene <484845..>486179
/gene="ERG9"
/locus_tag="YHR190W"
/db_xref="GeneID:856597"
mRNA <484845..>486179
/gene="ERG9"
/locus_tag="YHR190W"
/product="bifunctional farnesyl-diphosphate
farnesyltransferase/squalene synthase"
/transcript_id="NM_001179321.1"
/db_xref="GeneID:856597"
CDS 484845..486179
/gene="ERG9"
/locus_tag="YHR190W"
/EC_number="2.5.1.21"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:16407407]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:1807826|PMID:14562095|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0006696 ergosterol
biosynthetic process [PMID:8323279]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:350878]"
/experiment="EXISTENCE:direct assay:GO:0051996 squalene
synthase [NAD(P)H] activity [PMID:8323279|PMID:8157649]"
/experiment="EXISTENCE:mutant phenotype:GO:0006696
ergosterol biosynthetic process
[PMID:200835|PMID:1807826]"
/note="Farnesyl-diphosphate farnesyl transferase (squalene
synthase); joins two farnesyl pyrophosphate moieties to
form squalene in the sterol biosynthesis pathway"
/codon_start=1
/product="bifunctional farnesyl-diphosphate
farnesyltransferase/squalene synthase"
/protein_id="NP_012060.1"
/db_xref="GeneID:856597"
/db_xref="SGD:S000001233"
/translation="MGKLLQLALHPVEMKAALKLKFCRTPLFSIYDQSTSPYLLHCFE
LLNLTSRSFAAVIRELHPELRNCVTLFYLILRALDTIEDDMSIEHDLKIDLLRHFHEK
LLLTKWSFDGNAPDVKDRAVLTDFESILIEFHKLKPEYQEVIKEITEKMGNGMADYIL
DENYNLNGLQTVHDYDVYCHYVAGLVGDGLTRLIVIAKFANESLYSNEQLYESMGLFL
QKTNIIRDYNEDLVDGRSFWPKEIWSQYAPQLKDFMKPENEQLGLDCINHLVLNALSH
VIDVLTYLAGIHEQSTFQFCAIPQVMAIATLALVFNNREVLHGNVKIRKGTTCYLILK
SRTLRGCVEIFDYYLRDIKSKLAVQDPNFLKLNIQISKIEQFMEEMYQDKLPPNVKPN
ETPIFLKVKERSRYDDELVPTQQEEEYKFNMVLSIILSVLLGFYYIYTLHRA"
gene complement(<486230..>486631)
/gene="CTF8"
/locus_tag="YHR191C"
/db_xref="GeneID:856598"
mRNA complement(<486230..>486631)
/gene="CTF8"
/locus_tag="YHR191C"
/product="Ctf8p"
/transcript_id="NM_001179322.3"
/db_xref="GeneID:856598"
CDS complement(486230..486631)
/gene="CTF8"
/locus_tag="YHR191C"
/experiment="EXISTENCE:genetic interaction:GO:0007064
mitotic sister chromatid cohesion
[PMID:11389843|PMID:17483413]"
/experiment="EXISTENCE:mutant phenotype:GO:0007064 mitotic
sister chromatid cohesion [PMID:11389843]"
/experiment="EXISTENCE:mutant phenotype:GO:0034398
telomere tethering at nuclear periphery [PMID:16525505]"
/experiment="EXISTENCE:mutant phenotype:GO:0035753
maintenance of DNA trinucleotide repeats [PMID:21347277]"
/experiment="EXISTENCE:physical interaction:GO:0031390
Ctf18 RFC-like complex [PMID:11389843]"
/note="Subunit of a complex with Ctf18p; shares some
subunits with Replication Factor C; required for sister
chromatid cohesion"
/codon_start=1
/product="Ctf8p"
/protein_id="NP_012061.3"
/db_xref="GeneID:856598"
/db_xref="SGD:S000001234"
/translation="MPSVDIDASQWQKLTQSREKQTTVITPLGMMMLEIQGELELPKD
FASLARRDSPNEGRFSEQDGETLIRFGSLQIDGERATLFVGKKQRLLGKVTKLDVPMG
IMHFNSKDNKVELVDVMKYKVIFKDRPLPIM"
gene <486826..>487662
/gene="LNP1"
/locus_tag="YHR192W"
/db_xref="GeneID:856599"
mRNA <486826..>487662
/gene="LNP1"
/locus_tag="YHR192W"
/product="Lnp1p"
/transcript_id="NM_001179323.1"
/db_xref="GeneID:856599"
CDS 486826..487662
/gene="LNP1"
/locus_tag="YHR192W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0071782 endoplasmic
reticulum tubular network [PMID:22729086]"
/experiment="EXISTENCE:genetic interaction:GO:0034976
response to endoplasmic reticulum stress [PMID:27117666]"
/experiment="EXISTENCE:mutant phenotype:GO:0061709
reticulophagy [PMID:29915089]"
/experiment="EXISTENCE:mutant phenotype:GO:0071786
endoplasmic reticulum tubular network organization
[PMID:22729086]"
/note="Lunapark family member involved in ER network
formation; regulates the ER asymmetry-induced inheritance
block during ER stress; localizes to ER junctions and this
localization is regulated by the yeast atlastin ortholog
Sey1p; required for reticulophagy where it is proposed to
stabilize the actin-dependent remodeling of the ER;
interacts with the reticulon protein Rtn1p; induced in
response to the DNA-damaging agent MMS"
/codon_start=1
/product="Lnp1p"
/protein_id="NP_012062.1"
/db_xref="GeneID:856599"
/db_xref="SGD:S000001235"
/translation="MFSALGKWVRGSRNDKDFVTKYTADLSQITSQIHQLDVALKKSQ
SILSQWQSNLTFYGIALTVLALSYTYWEYHGYRPYLVVTALLCIGSLILFKWALTKLY
AFYNNNRLRKLAKLRAIHQKKLEKLKEETHYNATSSIIQRFSSGEDQNDDAMVLLDDE
LNAKYQELNNLKTELEKFKKESHVKGLKKEDSDAWFDKIISVLAGGNELDSTSSLSPF
KKIICPQCHWKSNCYRLASKPIIFICPHCNHKIDEVKEREDAIEAKQPAQPSQSEKEK
TK"
gene complement(<487712..>488236)
/gene="EGD2"
/locus_tag="YHR193C"
/db_xref="GeneID:856600"
mRNA complement(<487712..>488236)
/gene="EGD2"
/locus_tag="YHR193C"
/product="Egd2p"
/transcript_id="NM_001179324.3"
/db_xref="GeneID:856600"
CDS complement(487712..488236)
/gene="EGD2"
/locus_tag="YHR193C"
/experiment="EXISTENCE:direct assay:GO:0005854 nascent
polypeptide-associated complex [PMID:10219998]"
/experiment="EXISTENCE:direct assay:GO:0032266
phosphatidylinositol-3-phosphate binding [PMID:16343487]"
/experiment="EXISTENCE:direct assay:GO:0070273
phosphatidylinositol-4-phosphate binding [PMID:16343487]"
/experiment="EXISTENCE:direct assay:GO:0070300
phosphatidic acid binding [PMID:16343487]"
/experiment="EXISTENCE:direct assay:GO:0080025
phosphatidylinositol-3,5-bisphosphate binding
[PMID:16343487]"
/experiment="EXISTENCE:genetic interaction:GO:0006613
cotranslational protein targeting to membrane
[PMID:10518932]"
/experiment="EXISTENCE:mutant phenotype:GO:0051082
unfolded protein binding [PMID:9482879]"
/note="Alpha subunit of the nascent polypeptide-associated
complex (NAC); involved in protein sorting and
translocation; associated with cytoplasmic ribosomes"
/codon_start=1
/product="Egd2p"
/protein_id="NP_012063.3"
/db_xref="GeneID:856600"
/db_xref="SGD:S000001236"
/translation="MSAIPENANVTVLNKNEKKARELIGKLGLKQIPGIIRVTFRKKD
NQIYAIEKPEVFRSAGGNYVVFGEAKVDNFTQKLAAAQQQAQASGIMPSNEDVATKSP
EDIQADMQAAAEGSVNAAAEEDDEEGEVDAGDLNKDDIELVVQQTNVSKNQAIKALKA
HNGDLVNAIMSLSK"
gene <488657..>490396
/gene="MDM31"
/locus_tag="YHR194W"
/db_xref="GeneID:856601"
mRNA <488657..>490396
/gene="MDM31"
/locus_tag="YHR194W"
/product="Mdm31p"
/transcript_id="NM_001179325.1"
/db_xref="GeneID:856601"
CDS 488657..490396
/gene="MDM31"
/locus_tag="YHR194W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:15631992]"
/experiment="EXISTENCE:genetic interaction:GO:1900208
regulation of cardiolipin metabolic process
[PMID:22403410]"
/experiment="EXISTENCE:mutant phenotype:GO:0000001
mitochondrion inheritance [PMID:15631992]"
/experiment="EXISTENCE:mutant phenotype:GO:0006873
intracellular monoatomic ion homeostasis [PMID:16020778]"
/experiment="EXISTENCE:mutant phenotype:GO:0007005
mitochondrion organization [PMID:11907266|PMID:16020778]"
/note="Mitochondrial protein that may have a role in
phospholipid metabolism; inner membrane protein with
similarity to Mdm32p; required for normal mitochondrial
morphology and inheritance; interacts genetically with
MMM1, MMM2, MDM10, MDM12, and MDM34"
/codon_start=1
/product="Mdm31p"
/protein_id="NP_012064.1"
/db_xref="GeneID:856601"
/db_xref="SGD:S000001237"
/translation="MSLFTRPFLRSPRQFSVARYVYWARSPALRSNLRIPSIAAASLR
AYSNESKTGRDAPPDKKSKKLSNLKYITERDSLLVQTNNIFTKLKINIRWFLKKSTRP
FNSDDISAFISWILVSNIFIFIFWTTTFVSLILYLINTVFAQEYLASKIGKFITKNES
LSIVFESAIVPDWSSGKISFQKVFVSRRPKVSRGFTKGSQQDALQRAKLALSERILVN
QQDFDNGNYTQFDLTIDQVDISLNFRKWINGKGILDEVTINGLRGVIDRTHVVWKKDD
DPKNYLNVYQPGDFEISKFTMNDVLCTLYQPNGFRPFQVSIFNCDLPQLRKHWLFYDI
LNANNINGTYDNSMFTIHKKFRTDDQHQDPTLLWKQMTRFRVDNLDIDHLNAGIEGPF
GWINEGRVNMIGDVLLPDDDAASDSLQLTEILTEIGDRLIKKAKRYTSSLPLVGPGFS
PAVDEIDPNDYFIMDFSLRLYNVKAEVPLFTSGLTYINSALIRPIVGYINSHRTYIPI
KCRIVKKKSDFEGSWTIYDSYLMRDLSAEAYDAFADYVADDEKRTLRLRRVGFWSLQL
ILQVILMSLGAIA"
gene <490747..>491712
/gene="NVJ1"
/locus_tag="YHR195W"
/gene_synonym="VAB36"
/db_xref="GeneID:856602"
mRNA <490747..>491712
/gene="NVJ1"
/locus_tag="YHR195W"
/gene_synonym="VAB36"
/product="Nvj1p"
/transcript_id="NM_001179326.3"
/db_xref="GeneID:856602"
CDS 490747..491712
/gene="NVJ1"
/locus_tag="YHR195W"
/gene_synonym="VAB36"
/experiment="EXISTENCE:direct assay:GO:0005198 structural
molecule activity [PMID:28533415]"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:10888680]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:10888680]"
/experiment="EXISTENCE:direct assay:GO:0034399 nuclear
periphery [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0071561
nucleus-vacuole junction [PMID:10888680]"
/experiment="EXISTENCE:direct assay:GO:0071562
nucleus-vacuole junction assembly [PMID:10888680]"
/experiment="EXISTENCE:mutant phenotype:GO:0008104
intracellular protein localization [PMID:16912077]"
/experiment="EXISTENCE:mutant phenotype:GO:0034727
piecemeal microautophagy of the nucleus [PMID:12529432]"
/experiment="EXISTENCE:mutant phenotype:GO:0071562
nucleus-vacuole junction assembly [PMID:10888680]"
/note="Nuclear envelope protein; anchored to nuclear inner
membrane, interacts with vacuolar membrane protein Vac8p
to promote formation of nucleus-vacuole junctions during
piecemeal microautophagy of the nucleus (PMN); targeted to
vacuole via AP-3 pathway"
/codon_start=1
/product="Nvj1p"
/protein_id="NP_012065.3"
/db_xref="GeneID:856602"
/db_xref="SGD:S000001238"
/translation="MTRPPLVRGIFSLGLSVAVLKGVEKTVRKHLERQGWIEPQKVDY
ELIFTIDRLKNLVDNKREALTAEQPDAGELSWRKVFNFISRQSSELDTRIYVLILLLS
FLLPIAWTVLDGDRETTLEDKDNDCNVDLIENERRLKHYNDGERAVLQFGKNRSEPII
LSYKDMNVLEGEHEFTSKEEHSNSHLTSKSENALNQVGSEDLLGCHLEKQLEEDKNEP
NGEADGEDDNNREKDCSSSSEVESQSKCRKESTAEPDSLSRDTRTTSSLKSSTSFPIS
FKGSIDLKSLNQPSSLLHIQVSPTKSSNLDAQVNTEQAYSQPFRY"
gene <491931..>493658
/gene="UTP9"
/locus_tag="YHR196W"
/db_xref="GeneID:856603"
mRNA <491931..>493658
/gene="UTP9"
/locus_tag="YHR196W"
/product="Utp9p"
/transcript_id="NM_001179327.1"
/db_xref="GeneID:856603"
CDS 491931..493658
/gene="UTP9"
/locus_tag="YHR196W"
/experiment="EXISTENCE:direct assay:GO:0000049 tRNA
binding [PMID:19812255]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:12150911|PMID:11914276|PMID:12068309]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/experiment="EXISTENCE:direct assay:GO:0032040
small-subunit processome [PMID:12068309]"
/experiment="EXISTENCE:direct assay:GO:0033553 rDNA
heterochromatin [PMID:15489292]"
/experiment="EXISTENCE:direct assay:GO:0034455 t-UTP
complex [PMID:17515605]"
/experiment="EXISTENCE:direct assay:GO:0034511 U3 snoRNA
binding [PMID:12068309]"
/experiment="EXISTENCE:mutant phenotype:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12068309]"
/experiment="EXISTENCE:mutant phenotype:GO:0045943
positive regulation of transcription by RNA polymerase I
[PMID:15489292]"
/experiment="EXISTENCE:mutant phenotype:GO:0071528 tRNA
re-export from nucleus [PMID:19812255]"
/note="Nucleolar protein; component of the small subunit
(SSU) processome containing the U3 snoRNA that is involved
in processing of pre-18S rRNA"
/codon_start=1
/product="Utp9p"
/protein_id="NP_012066.1"
/db_xref="GeneID:856603"
/db_xref="SGD:S000001239"
/translation="MGSSLDLVASFSHDSTRFAFQASVAQKNNVDIYPLNETKDYVVN
SSLVSHIDYETNDMKVSDVIFFGWCSDLIDTQSSNIKRKLDEDEGTGESSEQRCENFF
VNGFPDGRIVVYSSNGKDIVNIIKNKKEILGADTDESDIWILDSDKVVKKLQYNNSKP
LKTFTLVDGKDDEIVHFQILHQNGTLLVCIITKQMVYIVDPSKRRPSTKYSFEISDAV
ACEFSSDGKYLLIANNEELIAYDLKEDSKLIQSWPVQVKTLKTLDDLIMALTTDGKIN
NYKIGEADKVCSIVVNEDLEIIDFTPINSKQQVLISWLNVNEPNFESISLKEIETQGY
ITINKNEKNNADEADQKKLEEKEEEAQPEVQHEKKETETKINKKVSKSDQVEIANILS
SHLEANSTEILDDLMSGSWTEPEIKKFILTKINTVDHLSKIFLTISKSITQNPWNEEN
LLPLWLKWLLTLKSGELNSIKDKHTKKNCKHLKSALRSSEEILPVLLGIQGRLEMLRR
QAKLREDLAQLSMQEGEDDEIEVIEHSNVISNPLQDQASPVEKLEPDSIVYANGESDE
FVDASEYKD"
gene <493896..>496187
/gene="RIX1"
/locus_tag="YHR197W"
/gene_synonym="IPI2"
/db_xref="GeneID:856604"
mRNA <493896..>496187
/gene="RIX1"
/locus_tag="YHR197W"
/gene_synonym="IPI2"
/product="Rix1p"
/transcript_id="NM_001179328.4"
/db_xref="GeneID:856604"
CDS 493896..496187
/gene="RIX1"
/locus_tag="YHR197W"
/gene_synonym="IPI2"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:22421151]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14690591|PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005654 nucleoplasm
[PMID:15528184|PMID:12374754]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0120330 rixosome
complex [PMID:14759368]"
/experiment="EXISTENCE:mutant phenotype:GO:0000027
ribosomal large subunit assembly [PMID:15528184]"
/experiment="EXISTENCE:mutant phenotype:GO:0006267
pre-replicative complex assembly involved in nuclear cell
cycle DNA replication [PMID:22421151]"
/experiment="EXISTENCE:mutant phenotype:GO:0006364 rRNA
processing [PMID:14759368]"
/experiment="EXISTENCE:mutant phenotype:GO:0030174
regulation of DNA-templated DNA replication initiation
[PMID:22421151]"
/note="Component of the Rix1 complex and possibly
pre-replicative complexes; required for processing of ITS2
sequences from 35S pre-rRNA; component of the pre-60S
ribosomal particle with the dynein-related AAA-type ATPase
Mdn1p; required for pre-replicative complex (pre-RC)
formation and maintenance during DNA replication
licensing; relocalizes to the cytosol in response to
hypoxia; essential gene"
/codon_start=1
/product="Rix1p"
/protein_id="NP_012067.4"
/db_xref="GeneID:856604"
/db_xref="SGD:S000001240"
/translation="MSEEFIAVSTLARNLEIAKGNEFHTILATLRSPVYINEQLLKSE
LSFLVTKILKLIRSGNDFDLWKGCHTSVVTCAYNPLVLSTHGGQLLAAIYSRLEQKTG
FYSSVISSSHGKQLFNTLISSVAIIIDLMKNKPTLSREALVPKLKAIIPTLITLSQYE
PELVLPVLQRILKRNTTTFKPFTNKFRTVLINLIISDYASLGTKTQRLVCENFAYLHL
LKIQVSDTSDDETQAHHKIYADSNWRTGLMSILSQFKPIIQLCGEILDFEQDNELYKL
IKSLPVIDESNNKEEFLPSLKLDFNAPLTLWEIPQRLSLLADMLVAFISLPTPFPIRV
PLGGINSLCEVLLGVSNKYLPLKKELRHDNELNGVINTILPQIQFQGIRLWEIMVSKY
GKCGLSFFEGILSSIELFIPLKKKSNNEIDFNVVGSLKFEFATVFRLVNMILSHLGHQ
LNIISVISQLIEVALFLSHDKTLIDSLFKNRKSIMKQQTKTKQSKRSKSAEGAFSDIY
THPELFVCKNSMNWFNEINDFFITALNNWILPSTPHIQILKYSITQSLRLKERFGYIP
ESFVNLLRCEVLHPGSERVSILPIAISLLKNINDDMFELLCHPKVPVGMVYQLHKPLD
LGEDGEVRDDINKKEVETNESSSNANTGLETLKALENLENVTIPEPKHEVPKVVDDTA
IFKKRSVEEVIERESTSSHKKVKFVEETTVDNGEELIVKKAVSQTKEEEKPMEDSEDE
EQEEFEIPAIELSDDEEEEEEEE"
gene complement(<496315..>497280)
/gene="AIM18"
/locus_tag="YHR198C"
/gene_synonym="FMP22"
/db_xref="GeneID:856605"
mRNA complement(<496315..>497280)
/gene="AIM18"
/locus_tag="YHR198C"
/gene_synonym="FMP22"
/product="Aim18p"
/transcript_id="NM_001179329.1"
/db_xref="GeneID:856605"
CDS complement(496315..497280)
/gene="AIM18"
/locus_tag="YHR198C"
/gene_synonym="FMP22"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:16823961|PMID:14562095|PMID:14576278|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:36739946]"
/experiment="EXISTENCE:direct assay:GO:0020037 heme
binding [PMID:36739946]"
/experiment="EXISTENCE:mutant phenotype:GO:0020037 heme
binding [PMID:36739946]"
/note="Hemoprotein; hypothetical protein that localizes to
the mitochondrial inner mnembrane; null mutant displays
elevated frequency of mitochondrial genome loss; contains
a chalcone isomerase (CHI) domain, a predicted N-terminal
transmembrane domain and an N-terminal mitochondrial
targeting sequence; lacks CHI catalytic activity"
/codon_start=1
/product="Aim18p"
/protein_id="NP_012068.1"
/db_xref="GeneID:856605"
/db_xref="SGD:S000001241"
/translation="MDRGRCANMLKSLQRTLAKCQKSPSTNHWQCFKRNFTSIRATKY
PGRSNSTFHYWPWFAASTLLATSLYYRDRPVQNDDKTDAFPSHTESIQVDSSVSDFPL
TITALNFPVSTTFKLLGYGQRHVTFLRFKVYALGLYLAENDENLVSDTLNETYLHKYF
LDVDDSKTPKENLARLLKRDDSKSVMMIDDLLDSGMRMLAKITPVRNTDFKHLKEGLV
KTISKHPDVANNKDTLAKGLSELNDAFSRKGSVRKNDDLIIELLANGALQFSYHDSKN
NEFEVMGVVNNQLVGKFLFSQYLCGEKSPSPQAKKTAIDKLITLL"
gene complement(<497490..>498422)
/gene="AIM46"
/locus_tag="YHR199C"
/gene_synonym="FMP34"
/db_xref="GeneID:856606"
mRNA complement(<497490..>498422)
/gene="AIM46"
/locus_tag="YHR199C"
/gene_synonym="FMP34"
/product="Aim46p"
/transcript_id="NM_001179330.3"
/db_xref="GeneID:856606"
CDS complement(497490..498422)
/gene="AIM46"
/locus_tag="YHR199C"
/gene_synonym="FMP34"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:14562095|PMID:16823961|PMID:14576278|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:36739946]"
/experiment="EXISTENCE:direct assay:GO:0020037 heme
binding [PMID:36739946]"
/experiment="EXISTENCE:mutant phenotype:GO:0020037 heme
binding [PMID:36739946]"
/note="Hemoprotein; hypothetical protein that localizes to
the mitochondrial inner mnembrane; null mutant displays
elevated frequency of mitochondrial genome loss; contains
a chalcone isomerase (CHI) domain, a predicted N-terminal
transmembrane domain and an N-terminal mitochondrial
targeting sequence; lacks CHI catalytic activity"
/codon_start=1
/product="Aim46p"
/protein_id="NP_012069.3"
/db_xref="GeneID:856606"
/db_xref="SGD:S000001242"
/translation="MRLISKVLVKTNCLEVGMRRAPQWYSHYSTTAGNARVNKKGSKV
VPVLTGLALASIFAKKWYDDSQIKKADATSVAVDASISAFPKKMGPPQWPFSTQYELI
GKGVRCVSSITFKAYGLGIYVAAEDKHLVSEVLDSKFLSQAFIDTAAPPSPENSHQDN
LRAALNDPAKAPILINNLLDSGIRLMSKNTPIKAGSFKLLMDGTKKSVLKNPDSQSQD
KDRLEAGFQELHDCFRSVKGLVARDDDFFIELNKDCSMNLSYYARKKDEFVILGTVKE
PLIGKLLFAHYLAAVDPPSPEARKEVIDALVSLS"
gene complement(<498571..>498859)
/gene="NBL1"
/locus_tag="YHR199C-A"
/db_xref="GeneID:2746858"
mRNA complement(join(<498571..498719,498787..>498859))
/gene="NBL1"
/locus_tag="YHR199C-A"
/product="borealin"
/transcript_id="NM_001184671.1"
/db_xref="GeneID:2746858"
CDS complement(join(498571..498719,498787..498859))
/gene="NBL1"
/locus_tag="YHR199C-A"
/experiment="EXISTENCE:direct assay:GO:0030674
protein-macromolecule adaptor activity [PMID:19158380]"
/experiment="EXISTENCE:direct assay:GO:0032133 chromosome
passenger complex [PMID:19158380]"
/experiment="EXISTENCE:mutant phenotype:GO:0007059
chromosome segregation [PMID:19158380]"
/note="Subunit of the conserved chromosomal passenger
complex (CPC); complex regulates mitotic chromosome
segregation; not required for the kinase activity of the
complex; mediates the interaction of Sli15p and Bir1p;
other complex members are Ipl1p, Sli15p, and Bir1p"
/codon_start=1
/product="borealin"
/protein_id="NP_976247.1"
/db_xref="GeneID:2746858"
/db_xref="SGD:S000029704"
/translation="MIPALTPEERQKLRSAILHRMQLELETTEKLIENIKEETLKKLN
LLQQPDATSAPQSKELIREVLEQEGRRIE"
gene <499079..>499885
/gene="RPN10"
/locus_tag="YHR200W"
/gene_synonym="MCB1; SUN1"
/db_xref="GeneID:856607"
mRNA <499079..>499885
/gene="RPN10"
/locus_tag="YHR200W"
/gene_synonym="MCB1; SUN1"
/product="proteasome regulatory particle base subunit
RPN10"
/transcript_id="NM_001179331.3"
/db_xref="GeneID:856607"
CDS 499079..499885
/gene="RPN10"
/locus_tag="YHR200W"
/gene_synonym="MCB1; SUN1"
/experiment="EXISTENCE:direct assay:GO:0008540 proteasome
regulatory particle, base subcomplex [PMID:9741626]"
/experiment="EXISTENCE:direct assay:GO:0036435 K48-linked
polyubiquitin modification-dependent protein binding
[PMID:8887631]"
/experiment="EXISTENCE:genetic interaction:GO:0043248
proteasome assembly [PMID:20941496]"
/experiment="EXISTENCE:mutant phenotype:GO:0000502
proteasome complex [PMID:15242647]"
/experiment="EXISTENCE:mutant phenotype:GO:0032436
positive regulation of proteasomal ubiquitin-dependent
protein catabolic process [PMID:40411784]"
/note="Proteasome polyubiquitin receptor; non-ATPase
subunit of the 19S regulatory particle (RP) of the 26S
proteasome that links the RP base to the lid; N-terminus
plays a role in the assembly of the regulatory particle
(RP); ubiquitin (Ub)-interacting motif selectively binds
to polyubiquitin chains and contributes to the allosteric
Ub sensor activity of Rpn11p that promotes substrate
engagement by the 26S proteasome; homolog of the mammalian
S5a protein"
/codon_start=1
/product="proteasome regulatory particle base subunit
RPN10"
/protein_id="NP_012070.3"
/db_xref="GeneID:856607"
/db_xref="SGD:S000001243"
/translation="MVLEATVLVIDNSEYSRNGDFPRTRFEAQIDSVEFIFQAKRNSN
PENTVGLISGAGANPRVLSTFTAEFGKILAGLHDTQIEGKLHMATALQIAQLTLKHRQ
NKVQHQRIVAFVCSPISDSRDELIRLAKTLKKNNVAVDIINFGEIEQNTELLDEFIAA
VNNPQEETSHLLTVTPGPRLLYENIASSPIILEEGSSGMGAFGGSGGDSDANGTFMDF
GVDPSMDPELAMALRLSMEEEQQRQERLRQQQQQQDQPEQSEQPEQHQDK"
gene complement(<499950..>501143)
/gene="PPX1"
/locus_tag="YHR201C"
/db_xref="GeneID:856608"
mRNA complement(<499950..>501143)
/gene="PPX1"
/locus_tag="YHR201C"
/product="exopolyphosphatase"
/transcript_id="NM_001179332.1"
/db_xref="GeneID:856608"
CDS complement(499950..501143)
/gene="PPX1"
/locus_tag="YHR201C"
/EC_number="3.6.1.11"
/experiment="EXISTENCE:direct assay:GO:0004309
exopolyphosphatase activity
[PMID:17215253|PMID:31175919|PMID:7860598]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:7860598]"
/experiment="EXISTENCE:direct assay:GO:0006798
polyphosphate catabolic process [PMID:8157625]"
/experiment="EXISTENCE:mutant phenotype:GO:0004309
exopolyphosphatase activity [PMID:7860598]"
/experiment="EXISTENCE:mutant phenotype:GO:0005759
mitochondrial matrix [PMID:12946255]"
/experiment="EXISTENCE:mutant phenotype:GO:0006798
polyphosphate catabolic process [PMID:7860598]"
/note="Exopolyphosphatase; hydrolyzes inorganic
polyphosphate (poly P) into Pi residues; located in the
cytosol, plasma membrane, and mitochondrial matrix"
/codon_start=1
/product="exopolyphosphatase"
/protein_id="NP_012071.1"
/db_xref="GeneID:856608"
/db_xref="SGD:S000001244"
/translation="MSPLRKTVPEFLAHLKSLPISKIASNDVLTICVGNESADMDSIA
SAITYSYCQYIYNEGTYSEEKKKGSFIVPIIDIPREDLSLRRDVMYVLEKLKIKEEEL
FFIEDLKSLKQNVSQGTELNSYLVDNNDTPKNLKNYIDNVVGIIDHHFDLQKHLDAEP
RIVKVSGSCSSLVFNYWYEKLQGDREVVMNIAPLLMGAILIDTSNMRRKVEESDKLAI
ERCQAVLSGAVNEVSAQGLEDSSEFYKEIKSRKNDIKGFSVSDILKKDYKQFNFQGKG
HKGLEIGLSSIVKRMSWLFNEHGGEADFVNQCRRFQAERGLDVLVLLTSWRKAGDSHR
ELVILGDSNVVRELIERVSDKLQLQLFGGNLDGGVAMFKQLNVEATRKQVVPYLEEAY
SNLEE"
rep_origin 501756..501996
/note="ARS822; Autonomously Replicating Sequence"
/db_xref="SGD:S000118362"
gene <502388..>504196
/gene="SMN1"
/locus_tag="YHR202W"
/db_xref="GeneID:856609"
mRNA <502388..>504196
/gene="SMN1"
/locus_tag="YHR202W"
/product="Smn1p"
/transcript_id="NM_001179333.3"
/db_xref="GeneID:856609"
CDS 502388..504196
/gene="SMN1"
/locus_tag="YHR202W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005576
extracellular region [PMID:35123996]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:11935221]"
/experiment="EXISTENCE:mutant phenotype:GO:0019677 NAD+
catabolic process [PMID:35123996]"
/note="Putative AMP hydrolase; secreted protein proposed
to function as an adenosine monophosphatase in NAD+
degradation, via nucleoside salvage pathway that converts
NAD+ to AMP and adenosine; overexpression results in
adenosine accumulation and reduced levels of NAD+,
deletion results in increased NAD+ and NADH; targeted to
vacuole via Vps10p-dependent endosomal vacuolar protein
sorting pathway; similar to human NT5E/CD73 gene involved
in cancer progression"
/codon_start=1
/product="Smn1p"
/protein_id="NP_012072.3"
/db_xref="GeneID:856609"
/db_xref="SGD:S000001245"
/translation="MILKLVHCLVALTGLIFAKPYQQQQAVLAPSQDVPLRDIHIGDI
NFIHTTDTHGWLGSHLSQNDYDADWGDFVAFVDILREKILRQSRDVIVIDTGDKRDGN
GLSDATWPPGLRSSEIFNMMDYDLLTLGNHELYTAESAILEYRGTSQSSKFKDKYVCS
NVEFIEDDGTRVPFGNKYITFETPIMKQRVLALSFLFSFQRANNRAIVTPPLEEITQK
SWFQNMVETNREEEIDLIIVFGHLPATDPTEREMHKIHALIRKYYPNTVIQYFGGHTH
IRDFVQLDSKSTCLQSGRFAETVGFLSINMTDPVDAESPIFSRRYIDFNKEAFKYHLS
KLGHDSNVPVSTKKGKTISRLVNDLRHELNLNEKLGYIPQTYYVSTRPLNSEENLYHL
ITHKILPNLIPPKNYEPSMSRFILINTGSVRYDLYKGPFTKDTEYIVMPFNNDWRFIT
VPLVVASRVETYLNKGPVIASLGIPSSSHHKQHFGGFQKCPFINNPNLSEGYTTEDDF
GCHGDDTPHNSQREYDIPNVVQCKEVKKVQEEEADPSKMVHVIFYSFMELDILNAVNS
IINDLGLRMENLTTNDCSHYGGDSTKKLLRDYFSQF"
gene complement(<504476..>505530)
/gene="RPS4B"
/locus_tag="YHR203C"
/db_xref="GeneID:856610"
mRNA complement(join(<504476..505247,505517..>505530))
/gene="RPS4B"
/locus_tag="YHR203C"
/product="40S ribosomal protein eS4 RPS4B"
/transcript_id="NM_001179334.1"
/db_xref="GeneID:856610"
CDS complement(join(504476..505247,505517..505530))
/gene="RPS4B"
/locus_tag="YHR203C"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:6814480]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:6814480]"
/experiment="EXISTENCE:direct assay:GO:0022627 cytosolic
small ribosomal subunit [PMID:6814480]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/note="Protein component of the small (40S) ribosomal
subunit; homologous to mammalian ribosomal protein S4, no
bacterial homolog; RPS4B has a paralog, RPS4A, that arose
from the whole genome duplication"
/codon_start=1
/product="40S ribosomal protein eS4 RPS4B"
/protein_id="NP_012073.1"
/db_xref="GeneID:856610"
/db_xref="SGD:S000001246"
/translation="MARGPKKHLKRLAAPHHWLLDKLSGCYAPRPSAGPHKLRESLPL
IVFLRNRLKYALNGREVKAILMQRHVKVDGKVRTDTTYPAGFMDVITLDATNENFRLV
YDVKGRFAVHRITDEEASYKLGKVKKVQLGKKGVPYVVTHDGRTIRYPDPNIKVNDTV
KIDLASGKITDFIKFDAGKLVYVTGGRNLGRIGTIVHKERHDGGFDLVHIKDSLDNTF
VTRLNNVFVIGEQGKPYISLPKGKGIKLSIAEERDRRRAQQGL"
gene <506319..>508709
/gene="MNL1"
/locus_tag="YHR204W"
/gene_synonym="HTM1"
/db_xref="GeneID:856611"
mRNA <506319..>508709
/gene="MNL1"
/locus_tag="YHR204W"
/gene_synonym="HTM1"
/product="alpha-1,2-mannosidase MNL1"
/transcript_id="NM_001179335.3"
/db_xref="GeneID:856611"
CDS 506319..508709
/gene="MNL1"
/locus_tag="YHR204W"
/gene_synonym="HTM1"
/EC_number="3.2.1.24"
/experiment="EXISTENCE:direct assay:GO:0004571
mannosyl-oligosaccharide 1,2-alpha-mannosidase activity
[PMID:21700223|PMID:27053108]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005788 endoplasmic
reticulum lumen [PMID:19279007]"
/experiment="EXISTENCE:direct assay:GO:0036508 protein
alpha-1,2-demannosylation [PMID:21700223]"
/experiment="EXISTENCE:direct assay:GO:1904382 mannose
trimming involved in glycoprotein ERAD pathway
[PMID:27053108]"
/experiment="EXISTENCE:genetic interaction:GO:1904382
mannose trimming involved in glycoprotein ERAD pathway
[PMID:21700223]"
/experiment="EXISTENCE:mutant phenotype:GO:0004571
mannosyl-oligosaccharide 1,2-alpha-mannosidase activity
[PMID:19124653]"
/experiment="EXISTENCE:mutant phenotype:GO:0030246
carbohydrate binding [PMID:11375935]"
/experiment="EXISTENCE:mutant phenotype:GO:0097466
ubiquitin-dependent glycoprotein ERAD pathway
[PMID:11375935|PMID:11254655]"
/experiment="EXISTENCE:mutant phenotype:GO:1904380
endoplasmic reticulum mannose trimming [PMID:27053108]"
/experiment="EXISTENCE:mutant phenotype:GO:1904382 mannose
trimming involved in glycoprotein ERAD pathway
[PMID:27053108|PMID:19124653]"
/note="Alpha-1,2-specific exomannosidase of the ER;
involved in glycan trimming of both folded and misfolded
glycoproteins; complexes with Pdi1p, and recognizes
misfolded, globular glycoproteins through the C-terminal
domain, trimming a mannose from Man8GlcNac2 glycan to
generate Man7GlcNac2, an oligosaccharide signal for
ER-associated protein degradation (ERAD); modifies Pdi1p
redox reactions; requires Pdi1p for stability and
substrate recognition; human homolog EDEM1 can complement
yeast null mutant"
/codon_start=1
/product="alpha-1,2-mannosidase MNL1"
/protein_id="NP_012074.3"
/db_xref="GeneID:856611"
/db_xref="SGD:S000001247"
/translation="MVCCLWVLLALLLHLDHVACEDDAYSFTSKELKAYKQEVKELFY
FGFDNYLEHGYPYDEVKPISCVPKKRNFEDPTDQGTNDILGNFTITLIDSLTTIAILE
DRPQFLKAVRLVERTFPDGNFDIDSTIQVFEITIRVIGSLLSSHLYATDPTKAVYLGD
DYDGSLLRLAQNMADRLLPAYLTSTGLPMPRRNIKRKWDVSEFPEFLETENNVAAMAS
PMFEFTILSYLTGDPKYEKVTRYAFDKTWSLRTGLDLLPMSFHPEKLTPYTPMTGIGA
SIDSLFEYALKGAILFDDSELMEVWNVAYEALKTNCKNDWFFANVMADTGHLFVPWID
SLSAFFSGLQVLAGDLDDAIANHLMFLKMWNTFGGIPERWNFSPPEFPPLSPLERSGA
VALDNILPLEWYPLRPEFFESTYFLYRATKDPFYLNIGVHLLKDLKQRFKSNCGFAGF
QNVITGELQDRMETFVLSETLKYLYLLFDEENELHNSASDVIFSTEAHPMWLPQEVRS
NYKRNAKFNNSVYSSHLEICQKKDREQAGENTLSQRIVGFAKSIFHKGPPDEEATDPI
IDYTIDTELPGTCSIKPHHVIGDEFWYSPMLSNFDRLFEIDSRFAATLIKPSHMHNYN
AIELEPGFYNRWSNPQFSTCLIPPTTEIFELLFDLPGYHQLNPLMLENKTITFETFGG
RSRLKIEKLQIYQIDYYGDLITASTFQDVSRKDIFSNACDAVASLYSPTYLYRVVAIN
GRILPRHGSVQIKKHSPVLTSNGTREEDEFKMDGIGINDHSQLMLECTPIINLFIV"
gene <509363..>511837
/gene="SCH9"
/locus_tag="YHR205W"
/gene_synonym="HRM2; KOM1"
/db_xref="GeneID:856612"
mRNA <509363..>511837
/gene="SCH9"
/locus_tag="YHR205W"
/gene_synonym="HRM2; KOM1"
/product="serine/threonine protein kinase SCH9"
/transcript_id="NM_001179336.1"
/db_xref="GeneID:856612"
CDS 509363..511837
/gene="SCH9"
/locus_tag="YHR205W"
/gene_synonym="HRM2; KOM1"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane
[PMID:17560372|PMID:19748353|PMID:15466158|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:17568771]"
/experiment="EXISTENCE:direct assay:GO:0004674 protein
serine/threonine kinase activity
[PMID:17568771|PMID:25921291]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:15466158]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:19159085]"
/experiment="EXISTENCE:genetic interaction:GO:0045943
positive regulation of transcription by RNA polymerase I
[PMID:19684113]"
/experiment="EXISTENCE:genetic interaction:GO:0045945
positive regulation of transcription by RNA polymerase III
[PMID:19684113]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:17568771]"
/experiment="EXISTENCE:mutant phenotype:GO:0008361
regulation of cell size [PMID:12089449]"
/experiment="EXISTENCE:mutant phenotype:GO:0032880
regulation of protein localization [PMID:19684113]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress [PMID:17568771]"
/experiment="EXISTENCE:mutant phenotype:GO:0045943
positive regulation of transcription by RNA polymerase I
[PMID:19684113]"
/experiment="EXISTENCE:mutant phenotype:GO:0045945
positive regulation of transcription by RNA polymerase III
[PMID:19684113]"
/experiment="EXISTENCE:mutant phenotype:GO:0047484
regulation of response to osmotic stress [PMID:17568771]"
/experiment="EXISTENCE:mutant phenotype:GO:0060963
positive regulation of ribosomal protein gene
transcription by RNA polymerase II [PMID:15466158]"
/experiment="EXISTENCE:mutant phenotype:GO:0090153
regulation of sphingolipid biosynthetic process
[PMID:24196832]"
/experiment="EXISTENCE:mutant phenotype:GO:1901494
regulation of cysteine metabolic process [PMID:31582588]"
/experiment="EXISTENCE:mutant phenotype:GO:1904828
positive regulation of hydrogen sulfide biosynthetic
process [PMID:31582588]"
/note="AGC family protein kinase; functional ortholog of
mammalian S6 kinase; phosphorylated by Tor1p and required
for TORC1-mediated regulation of ribosome biogenesis,
translation initiation, and entry into G0 phase; involved
in transactivation of osmostress-responsive genes;
regulates G1 progression, cAPK activity and nitrogen
activation of the FGM pathway; integrates nutrient signals
and stress signals from sphingolipids to regulate
lifespan"
/codon_start=1
/product="serine/threonine protein kinase SCH9"
/protein_id="NP_012075.1"
/db_xref="GeneID:856612"
/db_xref="SGD:S000001248"
/translation="MMNFFTSKSSNQDTGFSSQHQHPNGQNNGNNNSSTAGNDNGYPC
KLVSSGPCASSNNGALFTNFTLQTATPTTAISQDLYAMGTTGITSENALFQMKSMNNG
ISSVNNNNSNTPTIITTSQEETNAGNVHGDTGGNSLQNSEDDNFSSSSTTKCLLSSTS
SLSINQREAAAAAYGPDTDIPRGKLEVTIIEARDLVTRSKDSQPYVVCTFESSEFISN
GPESLGAINNNNNNNNNNQHNQNQHINNNNENTNPDAASQHHNNNSGWNGSQLPSIKE
HLKKKPLYTHRSSSQLDQLNSCSSVTDPSKRSSNSSSGSSNGPKNDSSHPIWHHKTTF
DVLGSHSELDISVYDAAHDHMFLGQVRLYPMIHNLAHASQHQWHSLKPRVIDEVVSGD
ILIKWTYKQTKKRHYGPQDFEVLRLLGKGTFGQVYQVKKKDTQRIYAMKVLSKKVIVK
KNEIAHTIGERNILVTTASKSSPFIVGLKFSFQTPTDLYLVTDYMSGGELFWHLQKEG
RFSEDRAKFYIAELVLALEHLHDNDIVYRDLKPENILLDANGNIALCDFGLSKADLKD
RTNTFCGTTEYLAPELLLDETGYTKMVDFWSLGVLIFEMCCGWSPFFAENNQKMYQKI
AFGKVKFPRDVLSQEGRSFVKGLLNRNPKHRLGAIDDGRELRAHPFFADIDWEALKQK
KIPPPFKPHLVSETDTSNFDPEFTTASTSYMNKHQPMMTATPLSPAMQAKFAGFTFVD
ESAIDEHVNNNRKFLQNSYFMEPGSFIPGNPNLPPDEDVIDDDGDEDINDGFNQEKNM
NNSHSQMDFDGDQHMDDEFVSGRFEI"
gene <512732..>514600
/gene="SKN7"
/locus_tag="YHR206W"
/gene_synonym="BRY1; POS9"
/db_xref="GeneID:856613"
mRNA <512732..>514600
/gene="SKN7"
/locus_tag="YHR206W"
/gene_synonym="BRY1; POS9"
/product="kinase-regulated stress-responsive transcription
factor SKN7"
/transcript_id="NM_001179337.3"
/db_xref="GeneID:856613"
CDS 512732..514600
/gene="SKN7"
/locus_tag="YHR206W"
/gene_synonym="BRY1; POS9"
/experiment="EXISTENCE:direct assay:GO:0000156
phosphorelay response regulator activity [PMID:11073911]"
/experiment="EXISTENCE:direct assay:GO:0003700 DNA-binding
transcription factor activity
[PMID:9118942|PMID:11854400]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10888672|PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0006368
transcription elongation by RNA polymerase II
[PMID:11854400|PMID:9118942]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding
[PMID:19111667|PMID:19158363]"
/experiment="EXISTENCE:mutant phenotype:GO:0000156
phosphorelay response regulator activity [PMID:11073911]"
/experiment="EXISTENCE:mutant phenotype:GO:0000304
response to singlet oxygen [PMID:16862604]"
/experiment="EXISTENCE:mutant phenotype:GO:0003700
DNA-binding transcription factor activity [PMID:9118942]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:17965252]"
/experiment="EXISTENCE:mutant phenotype:GO:0006368
transcription elongation by RNA polymerase II
[PMID:9118942]"
/experiment="EXISTENCE:mutant phenotype:GO:0008361
regulation of cell size [PMID:17302939]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress [PMID:17965252]"
/experiment="EXISTENCE:mutant phenotype:GO:1900101
regulation of endoplasmic reticulum unfolded protein
response [PMID:27373166]"
/note="Nuclear response regulator and transcription
factor; physically interacts with the Tup1-Cyc8 complex
and recruits Tup1p to its targets; part of a branched
two-component signaling system; required for optimal
induction of heat-shock genes in response to oxidative
stress; involved in osmoregulation; relocalizes to the
cytosol in response to hypoxia; SKN7 has a paralog, HMS2,
that arose from the whole genome duplication"
/codon_start=1
/product="kinase-regulated stress-responsive transcription
factor SKN7"
/protein_id="NP_012076.3"
/db_xref="GeneID:856613"
/db_xref="SGD:S000001249"
/translation="MSFSTINSNVNKTTGDSNNNTTENSSTADLLGMDLLQSGPRLMN
TMQPNNSSDMLHINNKTNNVQQPAGNTNISSANAGAKAPANEFVRKLFRILENNEYPD
IVTWTENGKSFVVLDTGKFTTHILPNHFKHSNFASFVRQLNKYDFHKVKRSPEERQRC
KYGEQSWEFQHPEFRVHYGKGLDNIKRKIPAQRKVLLDESQKALLHFNSEGTNPNNPS
GSLLNESTTELLLSNTVSKDAFGNLRRRVDKLQKELDMSKMESYATKVELQKLNSKYN
TVIESLITFKTINENLLNNFNTLCSTLANNGIEVPIFGDNGNRNPTGNTNPATTTAIQ
SNNNTNNASPATSTVSLQLPNLPDQNSLTPNAQNNTVTLRKGFHVLLVEDDAVSIQLC
SKFLRKYGCTVQVVSDGLSAISTLEKYRYDLVLMDIVMPNLDGATATSIVRSFDNETP
IIAMTGNIMNQDLITYLQHGMNDILAKPFTRDDLHSILIRYLKDRIPLCEQQLPPRNS
SPQTHSNTNTANSNPNTINEQSLAMLPQDNPSTTTPVTPGASISSAQHVQQGQQEQQH
QIFHAQQQQQHHNAIANARSDVAIPNLEHEINTVPHSSMGSTPQLPQSTLQENQLS"
gene complement(<514905..>516485)
/gene="SET5"
/locus_tag="YHR207C"
/db_xref="GeneID:856614"
mRNA complement(<514905..>516485)
/gene="SET5"
/locus_tag="YHR207C"
/product="S-adenosylmethionine-dependent
methyltransferase"
/transcript_id="NM_001179338.1"
/db_xref="GeneID:856614"
CDS complement(514905..516485)
/gene="SET5"
/locus_tag="YHR207C"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:22343720]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008757
S-adenosylmethionine-dependent methyltransferase activity
[PMID:22343720]"
/experiment="EXISTENCE:mutant phenotype:GO:0000723
telomere maintenance [PMID:27911222]"
/note="Methyltransferase involved in methylation of
histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent;
zinc-finger protein, contains one canonical and two
unusual fingers in unusual arrangements; deletion enhances
replication of positive-strand RNA virus"
/codon_start=1
/product="S-adenosylmethionine-dependent
methyltransferase"
/protein_id="NP_012077.1"
/db_xref="GeneID:856614"
/db_xref="SGD:S000001250"
/translation="MTLTIKIGTLNDSDQSAVHNGTENGSDFRKITPTEEEICDDVVL
LWKEEPGTEDATIQHLYDRITERNQSWKLSASRFRKILNEHHLYDTDLETVSLYKDKI
HFPKALDSDAKVEVKFIDDEHGRGLFAKRDFSKGQIILKENKPIVYIPPLDKLFLISN
GKACARCGKALYDLTQHKIMVHYLDCEVCKAIWCSEKCKKAHASLHELLYHSWRSNRI
DILHAGNWKRFVNYCEKYCFTAAFSVGLIYGSMLLDTTGEVKEQWQKLASISQRERIK
LRDASGIGSTFSLLNGTTVHTEEESDNGTKKGVEKNIDDETVWEKCYELFCGAFPKAS
EEIDFEKFLTMIGTFNINQYNGQVYHWISFINHDCEPNAYIEQVEEHEELRLHARKPI
KKGEQIRITYVNPLHGVRLRRRELRVNWGFLCQCDRCQNELSTFERVPNLEKKNADAN
LGVEKIDSNDSSEDGSKKSTGNRKSSMREAQPDLKEILKNGKEFELDIPETVDTQGNV
RKTSVRFDSNVSVAVDER"
gene <517532..>518713
/gene="BAT1"
/locus_tag="YHR208W"
/gene_synonym="ECA39; TWT1"
/db_xref="GeneID:856615"
mRNA <517532..>518713
/gene="BAT1"
/locus_tag="YHR208W"
/gene_synonym="ECA39; TWT1"
/product="branched-chain-amino-acid transaminase BAT1"
/transcript_id="NM_001179339.3"
/db_xref="GeneID:856615"
CDS 517532..518713
/gene="BAT1"
/locus_tag="YHR208W"
/gene_synonym="ECA39; TWT1"
/EC_number="2.6.1.42"
/experiment="EXISTENCE:direct assay:GO:0004084
branched-chain-amino-acid transaminase activity
[PMID:8798704]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:8798704]"
/experiment="EXISTENCE:direct assay:GO:0009082
branched-chain amino acid biosynthetic process
[PMID:8798704]"
/experiment="EXISTENCE:mutant phenotype:GO:0009082
branched-chain amino acid biosynthetic process
[PMID:8798704]"
/experiment="EXISTENCE:mutant phenotype:GO:0009083
branched-chain amino acid catabolic process
[PMID:8702755]"
/note="Mitochondrial branched-chain amino acid (BCAA)
aminotransferase; preferentially involved in BCAA
biosynthesis; homolog of murine ECA39; highly expressed
during logarithmic phase and repressed during stationary
phase; BAT1 has a paralog, BAT2, that arose from the whole
genome duplication"
/codon_start=1
/product="branched-chain-amino-acid transaminase BAT1"
/protein_id="NP_012078.3"
/db_xref="GeneID:856615"
/db_xref="SGD:S000001251"
/translation="MLQRHSLKLGKFSIRTLATGAPLDASKLKITRNPNPSKPRPNEE
LVFGQTFTDHMLTIPWSAKEGWGTPHIKPYGNLSLDPSACVFHYAFELFEGLKAYRTP
QNTITMFRPDKNMARMNKSAARICLPTFESEELIKLTGKLIEQDKHLVPQGNGYSLYI
RPTMIGTSKGLGVGTPSEALLYVITSPVGPYYKTGFKAVRLEATDYATRAWPGGVGDK
KLGANYAPCILPQLQAAKRGYQQNLWLFGPEKNITEVGTMNVFFVFLNKVTGKKELVT
APLDGTILEGVTRDSVLTLARDKLDPQEWDINERYYTITEVATRAKQGELLEAFGSGT
AAVVSPIKEIGWNNEDIHVPLLPGEQCGALTKQVAQWIADIQYGRVNYGNWSKTVADL
N"
gene <519437..>520312
/gene="CRG1"
/locus_tag="YHR209W"
/db_xref="GeneID:856616"
mRNA <519437..>520312
/gene="CRG1"
/locus_tag="YHR209W"
/product="S-adenosylmethionine-dependent
methyltransferase"
/transcript_id="NM_001179340.1"
/db_xref="GeneID:856616"
CDS 519437..520312
/gene="CRG1"
/locus_tag="YHR209W"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:20844764]"
/experiment="EXISTENCE:direct assay:GO:0008757
S-adenosylmethionine-dependent methyltransferase activity
[PMID:22028670]"
/experiment="EXISTENCE:mutant phenotype:GO:0008168
methyltransferase activity [PMID:22028670]"
/experiment="EXISTENCE:mutant phenotype:GO:0055088 lipid
homeostasis [PMID:22028670]"
/note="S-AdoMet-dependent methyltransferase involved in
lipid homeostasis; mediates resistance to a drug
cantharidin"
/codon_start=1
/product="S-adenosylmethionine-dependent
methyltransferase"
/protein_id="NP_012079.1"
/db_xref="GeneID:856616"
/db_xref="SGD:S000001252"
/translation="MPKTSYLNKNFESAHYNNVRPSYPLSLVNEIMKFHKGTRKSLVD
IGCGTGKATFVVEPYFKEVIGIDPSSAMLSIAEKETNERRLDKKIRFINAPGEDLSSI
RPESVDMVISAEAIHWCNLERLFQQVSSILRSDGTFAFWFYIQPEFVDFPEALNVYYK
YGWSKDYMGKYLNDNQREILLNYGGEKLRSLLSDRFGDIEVTIYSPSDPNASTVTAEN
SQFLWRAAITLNQFKEFVKSWSIYTSWARDNPSKPDIADIFINELKEICHCEDLNVPL
KIEWSTFYYLCRKRE"
gene complement(<520712..>521737)
/locus_tag="YHR210C"
/db_xref="GeneID:856617"
mRNA complement(<520712..>521737)
/locus_tag="YHR210C"
/product="aldose 1-epimerase superfamily protein"
/transcript_id="NM_001179341.3"
/db_xref="GeneID:856617"
CDS complement(520712..521737)
/locus_tag="YHR210C"
/note="Putative aldose 1-epimerase superfamily protein;
non-essential gene; highly expressed under anaeorbic
conditions"
/codon_start=1
/product="aldose 1-epimerase superfamily protein"
/protein_id="NP_012080.3"
/db_xref="GeneID:856617"
/db_xref="SGD:S000001253"
/translation="MSNNKAGGEYEVITIGDAKKLQATISELGATLLDLKVNNESIVL
GYPDIHGYISDGYNYIGATVGRYANRIYKGMFSMEDGPHQLTVNNCGNTNHSSISSFH
LKKYKASKVQNPLDDLYIVEFTLLDDRTLPNEFPGDLAVNLKYTLNVADMTLDLEYEA
KLVSGEATPINMTNHTYFNLNKTMDKKSISGTEVRLCSDKSLEVSEGALIPTGKIVQR
KIATFDSSKPTILQDDGPIYDYAFIVDENKNLKTTDSVSVNKLVPAFKAYHPASRLSL
EVSTTEPTVLFYTGDNLCDGFTPRSGFAVEQGRYVDAINRDGWRDCVLLRRGEVYTSK
TRYRFAV"
gene <525392..>528619
/gene="FLO5"
/locus_tag="YHR211W"
/db_xref="GeneID:856618"
mRNA <525392..>528619
/gene="FLO5"
/locus_tag="YHR211W"
/product="flocculin FLO5"
/transcript_id="NM_001179342.1"
/db_xref="GeneID:856618"
CDS 525392..528619
/gene="FLO5"
/locus_tag="YHR211W"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:9483793]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0000128
flocculation [PMID:19087208|PMID:19420680]"
/experiment="EXISTENCE:mutant phenotype:GO:0005537
D-mannose binding [PMID:2665372]"
/experiment="EXISTENCE:mutant phenotype:GO:0031589
cell-substrate adhesion [PMID:19087208]"
/note="Lectin-like cell wall protein (flocculin) involved
in flocculation; binds mannose chains on the surface of
other cells, confers floc-forming ability that is
chymotrypsin resistant but heat labile; important for
co-flocculation with other yeasts, mediating interaction
with specific species; FLO5 has a paralog, FLO1, that
arose from a segmental duplication"
/codon_start=1
/product="flocculin FLO5"
/protein_id="NP_012081.1"
/db_xref="GeneID:856618"
/db_xref="SGD:S000001254"
/translation="MTIAHHCIFLVILAFLALINVASGATEACLPAGQRKSGMNINFY
QYSLKDSSTYSNAAYMAYGYASKTKLGSVGGQTDISIDYNIPCVSSSGTFPCPQEDSY
GNWGCKGMGACSNSQGIAYWSTDLFGFYTTPTNVTLEMTGYFLPPQTGSYTFSFATVD
DSAILSVGGSIAFECCAQEQPPITSTNFTINGIKPWDGSLPDNITGTVYMYAGYYYPL
KVVYSNAVSWGTLPISVELPDGTTVSDNFEGYVYSFDDDLSQSNCTIPDPSIHTTSTI
TTTTEPWTGTFTSTSTEMTTITDTNGQLTDETVIVIRTPTTASTITTTTEPWTGTFTS
TSTEMTTVTGTNGQPTDETVIVIRTPTSEGLITTTTEPWTGTFTSTSTEMTTVTGTNG
QPTDETVIVIRTPTSEGLITTTTEPWTGTFTSTSTEVTTITGTNGQPTDETVIVIRTP
TSEGLITTTTEPWTGTFTSTSTEMTTVTGTNGQPTDETVIVIRTPTSEGLISTTTEPW
TGTFTSTSTEVTTITGTNGQPTDETVIVIRTPTSEGLITTTTEPWTGTFTSTSTEMTT
VTGTNGQPTDETVIVIRTPTSEGLITRTTEPWTGTFTSTSTEVTTITGTNGQPTDETV
IVIRTPTTAISSSLSSSSGQITSSITSSRPIITPFYPSNGTSVISSSVISSSVTSSLV
TSSSFISSSVISSSTTTSTSIFSESSTSSVIPTSSSTSGSSESKTSSASSSSSSSSIS
SESPKSPTNSSSSLPPVTSATTGQETASSLPPATTTKTSEQTTLVTVTSCESHVCTES
ISSAIVSTATVTVSGVTTEYTTWCPISTTETTKQTKGTTEQTKGTTEQTTETTKQTTV
VTISSCESDICSKTASPAIVSTSTATINGVTTEYTTWCPISTTESKQQTTLVTVTSCE
SGVCSETTSPAIVSTATATVNDVVTVYPTWRPQTTNEQSVSSKMNSATSETTTNTGAA
ETKTAVTSSLSRFNHAETQTASATDVIGHSSSVVSVSETGNTMSLTSSGLSTMSQQPR
STPASSMVGSSTASLEISTYAGSANSLLAGSGLSVFIASLLLAII"
repeat_region complement(530049..530379)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007000"
gene <538742..>538945
/locus_tag="YHR212W-A"
/db_xref="GeneID:1466531"
mRNA <538742..>538945
/locus_tag="YHR212W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184598.3"
/db_xref="GeneID:1466531"
CDS 538742..538945
/locus_tag="YHR212W-A"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/note="Pseudogenic fragment with similarity to flocculins;
identified by gene-trapping, microarray-based expression
analysis, and genome-wide homology searching; SWAT-GFP,
seamless-GFP and mCherry fusion proteins localize to the
endoplasmic reticulum; YHR212W-A has a paralog, YAR061W,
that arose from a segmental duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878091.3"
/db_xref="GeneID:1466531"
/db_xref="SGD:S000028650"
/translation="MPYHYLFLALFTYLATSNVVSGSTQACLPVGPRKNGMNVNFYKY
SLLDSTTYSYPQYMTSGYASNWN"
gene <539151..>539747
/locus_tag="YHR213W"
/db_xref="GeneID:856620"
mRNA <539151..>539747
/locus_tag="YHR213W"
/product="uncharacterized protein"
/transcript_id="NM_001179344.1"
/db_xref="GeneID:856620"
CDS 539151..539747
/locus_tag="YHR213W"
/note="Pseudogenic fragment with similarity to flocculins;
YHR213W has a paralog, YAR062W, that arose from a
segmental duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_012083.1"
/db_xref="GeneID:856620"
/db_xref="SGD:S000001256"
/translation="MTGYFLPPQTSSYTFRFAKVDDSAILSVGGNVAFECCAQEQPPI
TSTDFTINGIKPWQGSLPDNIGGTVYMYAGYYYPLKVVYSNAVSWGTLPISVELPDGT
TVSDDFEGYVYSFDDDLSQSNCTIPDPSKHTTSIVTTTTELWTGTFTSTSTEMTTVTG
TNGQPTDETVIVAKAPTTATSSSLSSSSSEQITSSITS"
gene <540549..>540782
/locus_tag="YHR213W-A"
/db_xref="GeneID:1466532"
mRNA <540549..>540782
/locus_tag="YHR213W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184599.3"
/db_xref="GeneID:1466532"
CDS 540549..540782
/locus_tag="YHR213W-A"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:40704575]"
/note="hypothetical protein; identified by gene-trapping,
microarray-based expression analysis, and genome-wide
homology searching"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878092.3"
/db_xref="GeneID:1466532"
/db_xref="SGD:S000028651"
/translation="MLAKTGDVVVQKVPVIRLSVFLHFFFVFPFCLLHRLYMGMKQVQ
EFIMEPKGSVFVVRATLRVSLENAGKIFFNETE"
gene <540800..>541099
/locus_tag="YHR213W-B"
/db_xref="GeneID:1466533"
mRNA <540800..>541099
/locus_tag="YHR213W-B"
/product="uncharacterized protein"
/transcript_id="NM_001184600.1"
/db_xref="GeneID:1466533"
CDS 540800..541099
/locus_tag="YHR213W-B"
/note="Pseudogenic fragment with similarity to flocculins;
identified by gene-trapping, microarray-based expression
analysis, and genome-wide homology searching; YHR213W-B
has a paralog, YAR064W, that arose from a segmental
duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878093.1"
/db_xref="GeneID:1466533"
/db_xref="SGD:S000028652"
/translation="MLIDFCCSYIAGTHGRERAPSFTGTFVSHVSAENNCRPRRSEIT
QPCASGTEKKHFAATEKQCTNSLEGSRKDFLSLPLGHSYLFLFCFWRMICSEPKL"
gene <541651..>542262
/locus_tag="YHR214W"
/db_xref="GeneID:856621"
mRNA <541651..>542262
/locus_tag="YHR214W"
/product="uncharacterized protein"
/transcript_id="NM_001179345.3"
/db_xref="GeneID:856621"
CDS 541651..542262
/locus_tag="YHR214W"
/note="hypothetical protein; predicted to be a
glycosylphosphatidylinositol-modified (GPI) protein;
YHR214W has a paralog, YAR066W, that arose from a
segmental duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_012084.3"
/db_xref="GeneID:856621"
/db_xref="SGD:S000001257"
/translation="MFNRFNKFQAAVALALLSRGALGDSYTNSTSSADLSSITSVSSA
SASATASDSLSSSDGTVYLPSTTISGDLTVTGKVIATEAVEVAAGGKLTLLDGEKYVF
SSDLKVHGDLVVEKSEASYEGTAFDVSGETFEVSGNFSAEETGAVSASIYSFTPSSFK
SSGDISLSLSKAKKGEVTFSPYSNAGTFSLSNAILNGGSVSGL"
mobile_element complement(543610..549637)
/note="YHRCTy1-1; Ty1 element, LTR retrotransposon of the
Copia (Pseudoviridae) group; contains co-transcribed genes
TYA Gag and TYB Pol, encoding proteins involved in
structure and function of virus-like particles, flanked by
two direct repeats"
/mobile_element_type="retrotransposon:YHRCTy1-1"
/db_xref="SGD:S000007006"
repeat_region complement(543610..543941)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007001"
gene complement(<543964..>549346)
/locus_tag="YHR214C-B"
/db_xref="GeneID:856623"
mRNA complement(<543964..>549346)
/locus_tag="YHR214C-B"
/product="gag-pol fusion protein"
/transcript_id="NM_001181431.2"
/db_xref="GeneID:856623"
CDS complement(join(543964..547929,547931..549346))
/locus_tag="YHR214C-B"
/EC_number="2.7.7.7"
/EC_number="2.7.7.49"
/EC_number="3.1.26.4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9448009]"
/ribosomal_slippage
/note="Retrotransposon TYA Gag and TYB Pol genes;
transcribed/translated as one unit; polyprotein is
processed to make a nucleocapsid-like protein (Gag),
reverse transcriptase (RT), protease (PR), and integrase
(IN); similar to retroviral genes; YHR214C-B has a
paralog, YAR070C, that arose from a segmental duplication"
/codon_start=1
/product="gag-pol fusion protein"
/protein_id="NP_012086.1"
/db_xref="GeneID:856623"
/db_xref="SGD:S000003534"
/translation="MESQQLSNYPHISHGSACASVTSKEVHTNQDPLDVSASKIQEYD
KASTKANSQQTTTPASSAVPENLHHASPQPASVPPPQNGPYPQQCMMTQNQANPSGWS
FYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVPPMLTSPND
FPNWVKTYIKFLQNSNLGGIIPTVNGKPVRQITDDELTFLYNTFQIFAPSQFLPTWVK
DILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQYNGSTPADAFETKVTNIIDR
LNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNMTVAELFLDIHAIYEEQQGSR
NSKPNYRRNPSDEKNDSRSYTNTTKPKVIARNPQKTNNSKSKTARAHNVSTSNNSPST
DNDSISKSTTEPIQLNNKHDLHLGQKLTESTVNHTNHSDDELPGHLLLDSGASRTLIR
SAHHIHSASSNPDINVVDAQKRNIPINAIGDLQFHFQDNTKTSIKVLHTPNIAYDLLS
LNELAAVDITACFTKNVLERSDGTVLAPIVKYGDFYWVSKKYLLPSNISVPTINNVHT
SESTRKYPYPFIHRMLAHANAQTIRYSLKNNTITYFNESDVDWSSAIDYQCPDCLIGK
STKHRHIKGSRLKYQNSYEPFQYLHTDIFGPVHNLPKSAPSYFISFTDETTKFRWVYP
LHDRREDSILDVFTTILAFIKNQFQASVLVIQMDRGSEYTNRTLHKFLEKNGITPCYT
TTADSRAHGVAERLNRTLLDDCRTQLQCSGLPNHLWFSAIEFSTIVRNSLASPKSKKS
ARQHAGLAGLDISTLLPFGQPVIVNDHNPNSKIHPRGIPGYALHPSRNSYGYIIYLPS
LKKTVDTTNYVILQGKESRLDQFNYDALTFDEDLNRLTASYHSFIASNEIQQSNDLNI
ESDHDFQSDIELHPEQLRNVLSKAVSPTDSTPPSTHTEDSKRVSKTNIRAPREVDPNI
SESNILPSKKRSSTPQISDIESTGSGGMHRLDVPLLAPMSQSNTHESSHASKSKDFRH
SDSYSDNETNHTNVPISSTGGTNNKTVPQTSEQETEKRIIHRSPSIDTSSSESNSLHH
VVPIKTSDTCPKENTEESIIADLPLPDLPPEPPTELSDSFKELPPINSHQTNSSLGGI
GDSNAYTTINSKKRSLEDNETEIKVSRDTWNTKNMRSLEPPRSKKRIHLIAAVKAVKS
IKPIRTTLRYDEAITYNKDIKEKEKYIQAYHKEVNQLLMMKTWDTDRYYDRKEIDPKR
VINSMFIFNRKRDGTHKARFVARGDIQHPDTYDPGMQSNTVHHYALMTSLSLALDNNY
YITQLDISSAYLYADIKEELYIRPPPHLGMNDKLIRLKKSLYGLKQSGANWYETIKSY
LIKQCGMEEVRGWSCVFKNSQVTICLFVDDMILFSKDLNANKKIITTLKKQYDTKIIN
LGESDNEIQYDILGLEIKYQRGKYMKLGMENSLTEKIPKLNVPLNPKGRKLSAPGQPG
LYIDQDELEIDEDEYKEKVHEMQKLIGLASYVGYKFRFDLLYYINTLAQHILFPSRQV
LDMTYELIQFMWDTRDKQLIWHKNKPTEPDNKLVAISDASYGNQPYYKSQIGNIYLLN
GKVIGGKSTKASLTCTSTTEAEIHAISESVPLLNNLSHLVQELNKKPITKGLLTDSKS
TISIIISNNEEKFRNRFFGTKAMRLRDEVSGNHLHVCYIETKKNIADVMTKPLPIKTF
KLLTNKWIH"
gene complement(<547910..>549346)
/locus_tag="YHR214C-C"
/db_xref="GeneID:856624"
mRNA complement(<547910..>549346)
/locus_tag="YHR214C-C"
/product="gag protein"
/transcript_id="NM_001184437.3"
/db_xref="GeneID:856624"
CDS complement(547910..549346)
/locus_tag="YHR214C-C"
/note="Retrotransposon TYA Gag gene co-transcribed with
TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is
a nucleocapsid protein that is the structural constituent
of virus-like particles (VLPs); similar to retroviral Gag"
/codon_start=1
/product="gag protein"
/protein_id="NP_036195.3"
/db_xref="GeneID:856624"
/db_xref="SGD:S000007421"
/translation="MESQQLSNYPHISHGSACASVTSKEVHTNQDPLDVSASKIQEYD
KASTKANSQQTTTPASSAVPENLHHASPQPASVPPPQNGPYPQQCMMTQNQANPSGWS
FYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVPPMLTSPND
FPNWVKTYIKFLQNSNLGGIIPTVNGKPVRQITDDELTFLYNTFQIFAPSQFLPTWVK
DILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQYNGSTPADAFETKVTNIIDR
LNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNMTVAELFLDIHAIYEEQQGSR
NSKPNYRRNPSDEKNDSRSYTNTTKPKVIARNPQKTNNSKSKTARAHNVSTSNNSPST
DNDSISKSTTEPIQLNNKHDLHLRPETY"
repeat_region complement(549306..549637)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007002"
gene complement(<550648..>550941)
/locus_tag="YHR214C-D"
/db_xref="GeneID:1466534"
mRNA complement(<550648..>550941)
/locus_tag="YHR214C-D"
/product="uncharacterized protein"
/transcript_id="NM_001184601.1"
/db_xref="GeneID:1466534"
CDS complement(550648..550941)
/locus_tag="YHR214C-D"
/note="hypothetical protein; identified by gene-trapping,
microarray-based expression analysis, and genome-wide
homology searching; SWAT-GFP and mCherry fusion proteins
localize to the nucleus and endoplasmic reticulum
respectively; YHR214C-D has a paralog, YAR069C, that arose
from a segmental duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878094.1"
/db_xref="GeneID:1466534"
/db_xref="SGD:S000028653"
/translation="MEDHTLVAIVVFFGNGEPFHVSLSVEMVFVLLLSSTRIHEVVVL
ICYKLQHATWSWGNMSKNFSLKPDISLSFLLDIISINDICIYGCIALTVVFIL"
gene complement(<551200..>551499)
/locus_tag="YHR214C-E"
/db_xref="GeneID:1466535"
mRNA complement(<551200..>551499)
/locus_tag="YHR214C-E"
/product="uncharacterized protein"
/transcript_id="NM_001184602.3"
/db_xref="GeneID:1466535"
CDS complement(551200..551499)
/locus_tag="YHR214C-E"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:40704575]"
/note="hypothetical protein; identified by gene-trapping,
microarray-based expression analysis, and genome-wide
homology searching"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878095.3"
/db_xref="GeneID:1466535"
/db_xref="SGD:S000028654"
/translation="MYKITTIYLWLKSYLSFFIGLDNLDFLTLIRFFQCRLQNKLGLQ
DILDFFCNLCGHSMVRTCNMVEAAQKQNRITFGSIYVKLHPLVKLCTGIVWAPRV"
gene <552099..>553502
/gene="PHO12"
/locus_tag="YHR215W"
/gene_synonym="PHO10"
/db_xref="GeneID:856625"
mRNA <552099..>553502
/gene="PHO12"
/locus_tag="YHR215W"
/gene_synonym="PHO10"
/product="acid phosphatase PHO12"
/transcript_id="NM_001179346.1"
/db_xref="GeneID:856625"
CDS 552099..553502
/gene="PHO12"
/locus_tag="YHR215W"
/gene_synonym="PHO10"
/EC_number="3.1.3.2"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0003993 acid
phosphatase activity [PMID:8817921]"
/note="One of three repressible acid phosphatases;
glycoprotein that is transported to the cell surface by
the secretory pathway; pregulated by phosphate starvation;
PHO12 has a paralog, PHO11, that arose from a segmental
duplication"
/codon_start=1
/product="acid phosphatase PHO12"
/protein_id="NP_012087.1"
/db_xref="GeneID:856625"
/db_xref="SGD:S000001258"
/translation="MLKSAVYSILAASLVNAGTIPLGKLSDIDKIGTQTEIFPFLGGS
GPYYSFPGDYGISRDLPESCEMKQVQMVGRHGERYPTVSKAKSIMTTWYKLSNYTGQF
SGALSFLNDDYEFFIRDTKNLEMETTLANSVNVLNPYTGEMNAKRHARDFLAQYGYMV
ENQTSFAVFTSNSNRCHDTAQYFIDGLGDKFNISLQTISEAESAGANTLSAHHSCPAW
DDDVNDDILKKYDTKYLSGIAKRLNKENKGLNLTSSDANTFFAWCAYEINARGYSDIC
NIFTKDELVRFSYGQDLETYYQTGPGYDVVRSVGANLFNASVKLLKESEVQDQKVWLS
FTHDTDILNYLTTIGIIDDQNNLTAEHVPFMENTFHRSWYVPQGARVYTEKFQCSNDT
YVRYVINDAVVPIETCSTGPGFSCEINDFYGYAEKRVAGTDFLKVCNVSSVSNSTELT
FFWDWNTKHYNDTLLKQ"
gene <554396..>555967
/gene="IMD2"
/locus_tag="YHR216W"
/gene_synonym="PUR5"
/db_xref="GeneID:856626"
mRNA <554396..>555967
/gene="IMD2"
/locus_tag="YHR216W"
/gene_synonym="PUR5"
/product="IMP dehydrogenase IMD2"
/transcript_id="NM_001179347.3"
/db_xref="GeneID:856626"
CDS 554396..555967
/gene="IMD2"
/locus_tag="YHR216W"
/gene_synonym="PUR5"
/EC_number="1.1.1.205"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:20097157]"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:20097157]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:23222640|PMID:14562095]"
/note="Inosine monophosphate dehydrogenase; catalyzes the
rate-limiting step in GTP biosynthesis, expression is
induced by mycophenolic acid resulting in resistance to
the drug, expression is repressed by nutrient limitation;
IMD2 has a paralog, YAR073W/YAR075W, that arose from a
segmental duplication"
/codon_start=1
/product="IMP dehydrogenase IMD2"
/protein_id="NP_012088.3"
/db_xref="GeneID:856626"
/db_xref="SGD:S000001259"
/translation="MAAIRDYKTALDFTKSLPRPDGLSVQELMDSKIRGGLTYNDFLI
LPGLVDFASSEVSLQTKLTRNITLNIPLVSSPMDTVTESEMATFMALLGGIGFIHHNC
TPEDQADMVRRVKNYENGFINNPIVISPTTTVGEAKSMKEKYGFAGFPVTTDGKRNAK
LVGVITSRDIQFVEDNSLLVQDVMTKNPVTGAQGITLSEGNEILKKIKKGRLLVVDEK
GNLVSMLSRTDLMKNQNYPLASKSANTKQLLCGASIGTMDADKERLRLLVKAGLDVVI
LDSSQGNSIFELNMLKWVKESFPGLEVIAGNVVTREQAANLIAAGADGLRIGMGTGSI
CITQEVMACGRPQGTAVYNVCEFANQFGVPCMADGGVQNIGHITKALALGSSTVMMGG
MLAGTTESPGEYFYQDGKRLKAYRGMGSIDAMQKTGTKGNASTSRYFSESDSVLVAQG
VSGAVVDKGSIKKFIPYLYNGLQHSCQDIGCRSLTLLKNNVQRGKVRFEFRTASAQLE
GGVHNLHSYEKRLHN"
rep_origin 556001..556331
/note="ARS824; Autonomously Replicating Sequence"
/db_xref="SGD:S000118363"
telomere 556105..562643
/note="TEL08R; Telomeric region on the right arm of
Chromosome VIII; composed of an X element core sequence, X
element combinatorial repeats, an internal stretch of
telomeric repeats, a short Y' element, and a terminal
stretch of telomeric repeats"
/db_xref="SGD:S000028964"
gene <558014..>559924
/locus_tag="YHR218W"
/db_xref="GeneID:856628"
mRNA join(<558014..558615,558715..>559924)
/locus_tag="YHR218W"
/product="uncharacterized protein"
/transcript_id="NM_001179349.1"
/db_xref="GeneID:856628"
CDS join(558014..558615,558715..559924)
/locus_tag="YHR218W"
/note="Helicase-like protein encoded within the telomeric
Y' element"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_012090.1"
/db_xref="GeneID:856628"
/db_xref="SGD:S000001261"
/translation="MDRKRSFEKIVVSVMVGKNVQKFLTFVEDEPDFQGGPIPSKYLI
PKKINLMVYTLFQVHTLKFNRKDYDTLSLFYLNRGYYNELSFRVLERCYEIASARPND
SSTMRTFTDFVSGTPIVRSLQKSTIRKYGYNLAPYMFLLLHVDELSIFSAYQASLPGE
KKVDTERLKRDLCPRKPTEIKYFSQICNDMMNKKDRLGDVLATAQRIRRRYNKNGSSE
PRLKTLDGLTSERWIQWLGLESDYHCSFSSTRNAEDVVAGEAASSDHDQKISRVTRKR
PREPKSTNDILVAGRKLFGSSFEFRDLHQLRLCHEIYMADTPSVAVQAPPGYGKTELF
HLPLIALASKGDVKYVSFLFVPYTVLLANCMIRLGRRGCLNVAPVRNFIEEGCDGVTD
LYVGIYDDLASTNFTDRIAAWENIVECTFRTNNVKLGYLIVDEFHNFETEVYRQSQFG
GITNLDFDAFEKAIFLSGTAPEAVADAALQRIGLTGLAKKSMDINELKRSEDLSRGLS
SYPTRMFNLIKEKSEVPLGHVHKIWKKVESQPEEALKLLLALFEIEPESKAIVVASTT
NEVEELACSWRKYFRVVWIHGKLGCCRKGVSHKGVCH"
gene <560173..>562047
/locus_tag="YHR219W"
/db_xref="GeneID:856629"
mRNA <560173..>562047
/locus_tag="YHR219W"
/product="uncharacterized protein"
/transcript_id="NM_001179350.3"
/db_xref="GeneID:856629"
CDS 560173..562047
/locus_tag="YHR219W"
/note="hypothetical protein with similarity to helicases;
located in the telomere region on the right arm of
chromosome VIII"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_012091.3"
/db_xref="GeneID:856629"
/db_xref="SGD:S000001262"
/translation="MDLNQRKEKKGQHVGCCGSRTDLSADTVELIERMDRLAENQATA
SMSIVALPSSFQESNSSDRCRKYCSSDEDSDTCIHGSANASTNATTNSSTNATTTASI
NVRTSATTTASINVRTSATTTESTNSNTNATTTESTNSSTNATTTASINVRTSATTTE
STNSNTSATTTESTDSNTSATTTESTDSNTSATTTASTNSSTNATTTASTNSSTNATT
TESTNASAKEDANKDGNAEDNRFHPVTDINKESYKRKGSQMVLLERKKLKAQFPNTSE
NMNVLQFLGFRSDEIKHLFLYGIDIYFCPEGVFTQYGLCKGCQKMFELCVCWAGQKVS
YRRMAWEALAVERMLRNDEEYKEYLEDIEPYHGDPVGYLKFFSVKRGEIYSQIQRNYA
WYLAITRRRETISVLDSTRGKQGSQVFRMSGRQIKELYYKVWSNLRESKTEVLQYFLN
WDEKKCREEWEAKDDTVFVEALEKVGVFQRLRSMTSAGLQGPQYVKLQFSRHHRQLRS
RYELSLGMHLRDQLALGVTPSKVPHWTAFLSMLIGLFYNKTFRQKLEYLLEQISEVWL
LPHWVDLANVEVLAADNTRVPLYMLMVAVHKELDSDDVPDGRFDIILLCRDSSREVGE
"
CONTIG join(BK006934.2:1..562643)
//
