LOCUS NC_001146 784333 bp DNA linear CON 23-JAN-2026
DEFINITION Saccharomyces cerevisiae S288C chromosome XIV, complete sequence.
ACCESSION NC_001146
VERSION NC_001146.8
DBLINK BioProject: PRJNA128
Assembly: GCF_000146045.2
KEYWORDS RefSeq.
SOURCE Saccharomyces cerevisiae S288C
ORGANISM Saccharomyces cerevisiae S288C
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
Saccharomyces.
REFERENCE 1 (bases 1 to 784333)
AUTHORS Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M.,
Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S.,
Weng,S. and Cherry,J.M.
TITLE New data and collaborations at the Saccharomyces Genome Database:
updated reference genome, alleles, and the Alliance of Genome
Resources
JOURNAL Genetics 220 (4) (2022)
PUBMED 34897464
REFERENCE 2 (bases 1 to 784333)
AUTHORS Philippsen,P., Kleine,K., Pohlmann,R., Dusterhoft,A., Hamberg,K.,
Hegemann,J.H., Obermaier,B., Urrestarazu,L.A., Aert,R.,
Albermann,K., Altmann,R., Andre,B., Baladron,V., Ballesta,J.P.,
Becam,A.M., Beinhauer,J., Boskovic,J., Buitrago,M.J., Bussereau,F.,
Coster,F., Crouzet,M., D'Angelo,M., Dal Pero,F., De Antoni,A.,
Hani,J. et al.
TITLE The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV
and its evolutionary implications
JOURNAL Nature 387 (6632 SUPPL), 93-98 (1997)
PUBMED 9169873
REFERENCE 3 (bases 1 to 784333)
AUTHORS Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
Oliver,S.G.
TITLE Life with 6000 genes
JOURNAL Science 274 (5287), 546 (1996)
PUBMED 8849441
REFERENCE 4 (bases 1 to 784333)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (23-JAN-2026) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 5 (bases 1 to 784333)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (04-MAY-2012) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Protein update by submitter
REFERENCE 6 (bases 1 to 784333)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (31-MAR-2011) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Sequence update by submitter
REFERENCE 7 (bases 1 to 784333)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (26-MAY-2010) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Sequence update by submitter
REFERENCE 8 (bases 1 to 784333)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (14-DEC-2009) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
COMMENT REVIEWED REFSEQ: This record has been curated by SGD. The reference
sequence is identical to BK006947.
On Apr 26, 2011 this sequence version replaced NC_001146.7.
##Genome-Annotation-Data-START##
Annotation Provider :: SGD
Annotation Status :: Full Annotation
Annotation Version :: R64-4-1
URL :: http://www.yeastgenome.org/
##Genome-Annotation-Data-END##
COMPLETENESS: full length.
FEATURES Location/Qualifiers
source 1..784333
/organism="Saccharomyces cerevisiae S288C"
/mol_type="genomic DNA"
/strain="S288C"
/db_xref="taxon:559292"
/chromosome="XIV"
telomere complement(1..7428)
/note="TEL14L; Telomeric region on the left arm of
Chromosome XIV; composed of an X element core sequence, X
element combinatorial repeats, a short stretch of
telomeric repeats, and a long Y' element"
/db_xref="SGD:S000028924"
gene complement(<371..>6098)
/gene="YRF1-6"
/locus_tag="YNL339C"
/gene_synonym="YRF1"
/db_xref="GeneID:855377"
mRNA complement(join(<371..5931,6080..>6098))
/gene="YRF1-6"
/locus_tag="YNL339C"
/gene_synonym="YRF1"
/product="Y' element ATP-dependent helicase protein 1 copy
6"
/transcript_id="NM_001183177.1"
/db_xref="GeneID:855377"
CDS complement(join(371..5931,6080..6098))
/gene="YRF1-6"
/locus_tag="YNL339C"
/gene_synonym="YRF1"
/experiment="EXISTENCE:curator inference:GO:0005634
nucleus [PMID:9837911]"
/experiment="EXISTENCE:genetic interaction:GO:0000722
telomere maintenance via recombination [PMID:9837911]"
/note="Helicase encoded by the Y' element of subtelomeric
regions; highly expressed in the mutants lacking the
telomerase component TLC1; potentially phosphorylated by
Cdc28p"
/codon_start=1
/product="Y' element ATP-dependent helicase protein 1 copy
6"
/protein_id="NP_014060.1"
/db_xref="GeneID:855377"
/db_xref="SGD:S000005283"
/translation="MEIENEQICTCIAQILHLLNSLIITFLDDDKTETGQSFVYIDGF
LVKKHNNQHTIVNFETYKNKMKVSDRRKFEKANFDEFESALNNKNDLVHCPSITLFES
IPTEVRSFYEDEKSGLIKVVKFRTGAMDRKRSFEKIVVSVMVGKNVQKFLTFVEDEPD
FQGGPIPSKYLIPKKINLMVYTLFQVHTLKFNRKDYDTLSLFYLNRGYYNELSFRVLE
RCYEIASARPNDSSTMRTFTDFVSGTPIVRGLQKSTIRKYGYNLAPYMFLLLHVDELS
IFSAYQASLPGEKKVDTERLKRDLCPRKPTEIKYFSQICNDMMNKKDRLGDILHIILR
ACALNFGAGPRGGAGDEEDRSITNEEPIIPSVDEHGLKVCKLRSPNTPRRLRKTLDAV
KALLVSSCACTARDLDIFDDNNGVAMWKWIKILYHEVAQETALKDSYRITLVPSSDGV
SVCGKLFNREYVRGFYFACKAQFDNLWEELNDCFYMPTVVDIASLILRNREVLFREPK
RGIDEYLENDSFLQMIPVKYREIVLPKLRRDTNKMTAALKNKVTVAIDELTVPLMWMI
HFAVGYPYRYPELQLLAFAGPQRNVYVDDTTRRIQLYTDYNKNGSSEPRLKTLDGLTS
DYVFYFVTVLRQMQICALGNSYDAFNHDPWMDVVGFEDPDQVTNRDISRIVLYSYMFL
NTAKGCLVEYATFRQYMRELPKNAPQKLNFREMRQGLIALGRHCVGSRFETDLYESAT
SELMANHSVQTGRNIYGVDSFSLTSVSGTTATLLQERASERWIQWLGLESDYHCSFSS
TRNAEDVVAGEAASSDHHQKISRVTRKRPREPKSTNDILVAGQKLFGSSFEFRDLHQL
RLCHEIYMADTPSVAVQAPPGYGKTELFHLPLIALASKGDVKYVSFLFVPYTVLLANC
MIRLSRCGCLNVAPVRNFIEEGCDGVTDLYVGIYDDLASTNFTDRIAAWENIVECTFR
TNNVKLGYLIVDEFHNFETEVYRQSQFGGITNLDFDAFEKAIFLSGTAPEAVADAALQ
RIGLTGLAKKSMDINELKRSEDLSRGLSSYPTRMFNLIKEKSEVPLGHVHKIWKKVES
QPEEALKLLLALFEIEPESKAIVVASTTNEVEELACSWRKYFRVVWIHGKLGAAEKVS
RTKEFVTDGSMRVLIGTKLVTEGIDIKQLMMVIMLDNRLNIIELIQGVGRLRDGGLCY
LLSRKNSWAARNRKGELPPIKEGCITEQVREFYGLESKKGKKGQHVGCCGSRTDLSAD
TVELIERMDRLAEKQATASMSIVALPSSFQESNSSDRCRKYCSSDEDSDTCIHGSANA
STNATTNSSTNATTTASTNVRTSATTTASINVRTSATTTESTNSSTNATTTASTNVRT
SATTTASINVRTSATTTESTNSNTSATTTESTDSNTSATTTESTDSNTSATTTASTNS
STNATTTASTNSSTNATTTESTNASAKEDANKDGNAEDNRFHPVTDINKESYKRKGSQ
MVLLERKKLKAQFPNTSENMNVLQFLGFRSDEIKHLFLYGIDVYFCPEGVFTQYGLCK
GCQKMFELCVCWAGQKVSYRRMAWEALAVERMLRNDEEYKEYLEDIEPYHGDPVGYLK
FFSVKRGEIYSQIQRNYAWYLAITRRRETISVLDSTRGKQGSQVFRMSGRQIKELYYK
VWSNLRESKTEVLQYFLNWDEKKCREEWEAKDDTVFVEALEKVGVFQRLRSMTSAGLQ
GPQYVKLQFSRHHRQLRSRYELSLGMHLRDQLALGVTPSKVPHWTAFLSMLIGLFCNK
TFRQKLEYLLEQISEVWLLPHWLDLANVEVLAADNTRVPLYMLMVAVHKELDSDDVPD
GRFDILLCRDSSREVGE"
gene <8330..>9475
/gene="COS1"
/locus_tag="YNL336W"
/db_xref="GeneID:855380"
mRNA <8330..>9475
/gene="COS1"
/locus_tag="YNL336W"
/product="Cos1p"
/transcript_id="NM_001183174.1"
/db_xref="GeneID:855380"
CDS 8330..9475
/gene="COS1"
/locus_tag="YNL336W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:25942624]"
/note="Endosomal protein involved in turnover of plasma
membrane proteins; member of the DUP380 subfamily of
conserved, often subtelomeric COS genes; required for the
multivesicular vesicle body sorting pathway that
internalizes plasma membrane proteins for degradation; Cos
proteins provide ubiquitin in trans for nonubiquitinated
cargo proteins"
/codon_start=1
/product="Cos1p"
/protein_id="NP_014063.1"
/db_xref="GeneID:855380"
/db_xref="SGD:S000005280"
/translation="MKENELKNEKSVDVLSFKQLESQKIVLPQDLFRSSFTWFCYEIY
KSLAFRIWMLLWLPLSVWWKLSNNCIYPLIVSLLVLFLGPIFVLVICGLSRKRSLSKQ
LIQFCKEVTENTPSSDPHDWEVVAANLNSYLYENKAWNTKYFFFNAMVCQEAFRTTLL
EPFSLKKDEAAKVKSFKDSVPYIEEALGVYFREVEKQWKLFNSEKSWSPVGLEDAKLP
KEAYRFKLTWFLKRISNIFMLIPFLNFLCCIYVSRGMCLLLRTFYLGWILFMLVQGFQ
NMRMIVLSVKMEHKMQFLSTIINEQESGANGWDEIAKKMNRYLFEKKVWKNEEFFFDG
IDCEWFFSHFFYRVLSAKKSMRALSLNVELWPYIKEAQLSCSEESLA"
gene <11452..>12129
/gene="DDI2"
/locus_tag="YNL335W"
/gene_synonym="DDI3"
/db_xref="GeneID:855381"
mRNA <11452..>12129
/gene="DDI2"
/locus_tag="YNL335W"
/gene_synonym="DDI3"
/product="cyanamide hydratase"
/transcript_id="NM_001183173.1"
/db_xref="GeneID:855381"
CDS 11452..12129
/gene="DDI2"
/locus_tag="YNL335W"
/gene_synonym="DDI3"
/EC_number="4.2.1.69"
/experiment="EXISTENCE:direct assay:GO:0018820 cyanamide
hydratase activity [PMID:25847245]"
/experiment="EXISTENCE:mutant phenotype:GO:0018890
cyanamide metabolic process [PMID:25847245]"
/note="Cyanamide hydratase that detoxifies cyanamide;
member of the HD domain metalloprotein superfamily;
expression is induced over 100-fold by cyanamide and by
SN2-type DNA alkylating agents such as MMS and DMA;
induction decreased in rad6 and rad18 mutants; gene and
protein are identical to DDI3 and Ddi3p"
/codon_start=1
/product="cyanamide hydratase"
/protein_id="NP_014064.1"
/db_xref="GeneID:855381"
/db_xref="SGD:S000005279"
/translation="MSQYGFVRVPREVEKAIPVVNAPRPRAVVPPPNSETARLVREYA
AKELTAPVLNHSLRVFQYSVAIIRDQFPAWDLDQEVLYVTCLLHDIATTDKNMRATKM
SFEYYGGILSRELVFNATGGNQDYADAVTEAIIRHQDLTGTGYITTLGLILQIATTLD
NVGSNTDLIHIDTVSAINEQFPRLHWLSCFATVVDTENSRKPWGHTSSLGDDFSKKVI
CNTFGYN"
gene complement(<12208..>12876)
/gene="SNO2"
/locus_tag="YNL334C"
/db_xref="GeneID:855382"
mRNA complement(<12208..>12876)
/gene="SNO2"
/locus_tag="YNL334C"
/product="putative pyridoxal 5'-phosphate synthase"
/transcript_id="NM_001183172.1"
/db_xref="GeneID:855382"
CDS complement(12208..12876)
/gene="SNO2"
/locus_tag="YNL334C"
/EC_number="3.5.1.2"
/EC_number="4.3.3.6"
/note="hypothetical protein; nearly identical to Sno3p;
expression is induced before the diauxic shift and also in
the absence of thiamin"
/codon_start=1
/product="putative pyridoxal 5'-phosphate synthase"
/protein_id="NP_014065.1"
/db_xref="GeneID:855382"
/db_xref="SGD:S000005278"
/translation="MTVVIGVLALQGAFIEHVRHVEKCIVENRDFYEKKLSVMTVKDK
NQLAQCDALIIPGGESTAMSLIAERTGFYDDLYAFVHNPSKVTWGTCAGMIYISQQLS
NEEKLVKTLNLLKVKVKRNAFGRQAQSSTRICDFSNFIPHCNDFPATFIRAPVIEEVL
DPEHVQVLYKLDGKDNGGQELIVAAKQKNNILATSFHPELAENDIRFHDWFIREFVLK
NYSK"
gene <13267..>14163
/gene="SNZ2"
/locus_tag="YNL333W"
/db_xref="GeneID:855383"
mRNA <13267..>14163
/gene="SNZ2"
/locus_tag="YNL333W"
/product="pyridoxine biosynthesis protein SNZ2"
/transcript_id="NM_001183171.1"
/db_xref="GeneID:855383"
CDS 13267..14163
/gene="SNZ2"
/locus_tag="YNL333W"
/EC_number="4.3.3.6"
/experiment="EXISTENCE:genetic interaction:GO:0008615
pyridoxine biosynthetic process [PMID:30498136]"
/experiment="EXISTENCE:genetic interaction:GO:0009228
thiamine biosynthetic process [PMID:30498136]"
/experiment="EXISTENCE:genetic interaction:GO:0042819
vitamin B6 biosynthetic process [PMID:12271461]"
/note="Protein involved in thiamine and pyridoxine
biosynthesis; member of a stationary phase-induced gene
family where transcriptional induction precedes the
diauxic shift; induced in the absence of thiamin in a
Thi2/3p-dependent manner and repressed in its presence;
forms a co-regulated gene pair with SNO2; interacts with
Thi11p; paralog of SNZ1 and SNZ3"
/codon_start=1
/product="pyridoxine biosynthesis protein SNZ2"
/protein_id="NP_014066.1"
/db_xref="GeneID:855383"
/db_xref="SGD:S000005277"
/translation="MSEFKVKTGLAQMLKGGVIMDVVTPEQAIIAERAGACAVMALER
IPADMRKSGQVCRMSDPRMIKEIMEAVSIPVMAKVRIGHFVEAQILEELQVDYIDESE
VLTPADWTHHIEKHNFKVPFVCGAKDLGEALRRINEGAAMIRTKGEAGTGDVSEAVKH
ITKIKAEIQQYKENLKTESDFAAKATELRVPVDLLKTTLSEGKLPVVNFAAGGVATPA
DAALLMQLGCEGVFVGSGIFKSSDPEKLACAIVEATTHYDNPAKLLQISSDLGDLMGG
ISIQSINEAGGKNGARLSEIGW"
gene <14832..>15854
/gene="THI12"
/locus_tag="YNL332W"
/db_xref="GeneID:855384"
mRNA <14832..>15854
/gene="THI12"
/locus_tag="YNL332W"
/product="4-amino-5-hydroxymethyl-2-methylpyrimidine
phosphate synthase"
/transcript_id="NM_001183170.1"
/db_xref="GeneID:855384"
CDS 14832..15854
/gene="THI12"
/locus_tag="YNL332W"
/experiment="EXISTENCE:genetic interaction:GO:0009228
thiamine biosynthetic process [PMID:12777485]"
/note="Protein involved in synthesis of the thiamine
precursor HMP; member of a subtelomeric gene family
including THI5, THI11, THI12, and THI13;
hydroxymethylpyrimidine is also known as HMP"
/codon_start=1
/product="4-amino-5-hydroxymethyl-2-methylpyrimidine
phosphate synthase"
/protein_id="NP_014067.1"
/db_xref="GeneID:855384"
/db_xref="SGD:S000005276"
/translation="MSTDKITFLLNWQPTPYHIPIFLAQTKGYFKEQGLDMAILEPTN
PSDVTELIGSGKVDMGLKAMIHTLAAKARGFPVTSVASLLDEPFTGVLYLKGSGITED
FQSLKGKKIGYVGEFGKIQIDELTKHYGMKPEDYTAVRCGMNVAKYIIEGKIDAGIGI
ECMQQVELEEYLAKQGRPASDAKMLRIDKLACLGCCCFCTVLYICNDEFLKKNPEKVR
KFLKAIKKATDYVLADPVKAWKEYIDFKPRLNNDLSYKQYQRCYAYFSSSLYNVHRDW
KKVTGYGKRLAILPPDYVSNYTNEYLSWPEPEEVSDPLEAQRLMAIHQEKCRQEGTFK
RLALPA"
gene complement(<16118..>17248)
/gene="AAD14"
/locus_tag="YNL331C"
/db_xref="GeneID:855385"
mRNA complement(<16118..>17248)
/gene="AAD14"
/locus_tag="YNL331C"
/product="putative aryl-alcohol dehydrogenase"
/transcript_id="NM_001183169.1"
/db_xref="GeneID:855385"
CDS complement(16118..17248)
/gene="AAD14"
/locus_tag="YNL331C"
/experiment="EXISTENCE:direct assay:GO:0047681
aryl-alcohol dehydrogenase (NADP+) activity
[PMID:29079624]"
/note="Putative aryl-alcohol dehydrogenase; similar to P.
chrysosporium aryl-alcohol dehydrogenase; mutational
analysis has not yet revealed a physiological role;
members of the AAD gene family comprise three pairs (AAD3
+ AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14) whose two genes
are more related to one another than to other members of
the family"
/codon_start=1
/product="putative aryl-alcohol dehydrogenase"
/protein_id="NP_014068.1"
/db_xref="GeneID:855385"
/db_xref="SGD:S000005275"
/translation="MTDLFKPLPEPPTELGRLRVLSKTAGIRVSPLILGGASIGDAWS
GFMGSMNKEQAFELLDAFYEAGGNCIDTANSYQNEESEIWIGEWMASRKLRDQIVIAT
KFTGDYKKYEVGGGKSANYCGNHKRSLHVSVRDSLRKLQTDWIDILYIHWWDYMSSIE
EVMDSLHILVQQGKVLYLGVSDTPAWVVSAANYYATSHGKTPFSVYQGKWNVLNRDFE
RDIIPMARHFGMALAPWDVMGGGRFQSKKAMEERKKNGEGLRTFVGGPEQTELEVKIS
EALTKIAEEHGTESVTAIAIAYVRSKAKNVFPLIGGRKIEHLKQNIEALSIKLTPEQI
EYLESIVPFDVGFPKSLIGDDPAVTKKLSPLTSMSARIAFDN"
gene complement(<18001..>19302)
/gene="RPD3"
/locus_tag="YNL330C"
/gene_synonym="MOF6; REC3; SDI2; SDS6"
/db_xref="GeneID:855386"
mRNA complement(<18001..>19302)
/gene="RPD3"
/locus_tag="YNL330C"
/gene_synonym="MOF6; REC3; SDI2; SDS6"
/product="histone deacetylase RPD3"
/transcript_id="NM_001183168.1"
/db_xref="GeneID:855386"
CDS complement(18001..19302)
/gene="RPD3"
/locus_tag="YNL330C"
/gene_synonym="MOF6; REC3; SDI2; SDS6"
/EC_number="3.5.1.98"
/experiment="EXISTENCE:direct assay:GO:0000118 histone
deacetylase complex [PMID:8962081]"
/experiment="EXISTENCE:direct assay:GO:0004407 histone
deacetylase activity [PMID:12110674]"
/experiment="EXISTENCE:direct assay:GO:0032221 Rpd3S
complex [PMID:16286007|PMID:16286008]"
/experiment="EXISTENCE:direct assay:GO:0033698 Rpd3L
complex
[PMID:16286007|PMID:16314178|PMID:16286008|PMID:19040720]"
/experiment="EXISTENCE:direct assay:GO:0034399 nuclear
periphery [PMID:25817432]"
/experiment="EXISTENCE:direct assay:GO:0070210
Rpd3L-Expanded complex [PMID:19040720]"
/experiment="EXISTENCE:direct assay:GO:0070211 Snt2C
complex [PMID:19040720]"
/experiment="EXISTENCE:direct assay:GO:0070822 Sin3-type
complex [PMID:9234741]"
/experiment="EXISTENCE:genetic interaction:GO:0000082 G1/S
transition of mitotic cell cycle [PMID:19823668]"
/experiment="EXISTENCE:genetic interaction:GO:0000086 G2/M
transition of mitotic cell cycle [PMID:17908798]"
/experiment="EXISTENCE:genetic interaction:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:11069890]"
/experiment="EXISTENCE:genetic interaction:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:19823668|PMID:17908798]"
/experiment="EXISTENCE:genetic interaction:GO:0006368
transcription elongation by RNA polymerase II
[PMID:19948887]"
/experiment="EXISTENCE:genetic interaction:GO:0030174
regulation of DNA-templated DNA replication initiation
[PMID:15143171]"
/experiment="EXISTENCE:genetic interaction:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:17210643|PMID:17296735]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:24358376|PMID:17121596|PMID:20398213|PMID:15141165|P
MID:24881874|PMID:16314178|PMID:17158929]"
/experiment="EXISTENCE:mutant phenotype:GO:0003713
transcription coactivator activity [PMID:14737171]"
/experiment="EXISTENCE:mutant phenotype:GO:0003714
transcription corepressor activity [PMID:9150136]"
/experiment="EXISTENCE:mutant phenotype:GO:0004407 histone
deacetylase activity
[PMID:9572144|PMID:12110674|PMID:8962081|PMID:9512514]"
/experiment="EXISTENCE:mutant phenotype:GO:0006995
cellular response to nitrogen starvation [PMID:24881874]"
/experiment="EXISTENCE:mutant phenotype:GO:0016239
positive regulation of macroautophagy [PMID:22539722]"
/experiment="EXISTENCE:mutant phenotype:GO:0016479
negative regulation of transcription by RNA polymerase I
[PMID:19270272|PMID:14609951]"
/experiment="EXISTENCE:mutant phenotype:GO:0030174
regulation of DNA-templated DNA replication initiation
[PMID:19417103|PMID:12453428|PMID:15143171]"
/experiment="EXISTENCE:mutant phenotype:GO:0034503 protein
localization to nucleolar rDNA repeats [PMID:17203076]"
/experiment="EXISTENCE:mutant phenotype:GO:0034605
cellular response to heat [PMID:20398213]"
/experiment="EXISTENCE:mutant phenotype:GO:0044804
nucleophagy [PMID:31553911]"
/experiment="EXISTENCE:mutant phenotype:GO:0045128
negative regulation of reciprocal meiotic recombination
[PMID:18515193]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:20398213|PMID:15254041|PMID:17296735|PMID:17210643|P
MID:17706600]"
/experiment="EXISTENCE:mutant phenotype:GO:0051321 meiotic
cell cycle [PMID:17158929]"
/experiment="EXISTENCE:mutant phenotype:GO:0061186
negative regulation of silent mating-type cassette
heterochromatin formation
[PMID:10512855|PMID:19372273|PMID:10388812]"
/experiment="EXISTENCE:mutant phenotype:GO:0061188
negative regulation of rDNA heterochromatin formation
[PMID:10082585|PMID:10388812|PMID:10512855]"
/experiment="EXISTENCE:mutant phenotype:GO:0070550 rDNA
chromatin condensation [PMID:35477092|PMID:31553911]"
/experiment="EXISTENCE:physical interaction:GO:0000082
G1/S transition of mitotic cell cycle [PMID:19823668]"
/experiment="EXISTENCE:physical interaction:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:11069890]"
/experiment="EXISTENCE:physical interaction:GO:0003713
transcription coactivator activity [PMID:14737171]"
/experiment="EXISTENCE:physical interaction:GO:0003714
transcription corepressor activity [PMID:9150136]"
/experiment="EXISTENCE:physical interaction:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:19823668]"
/note="Histone deacetylase, component of both Rpd3S and
Rpd3L complexes; regulates transcription, silencing,
autophagy and other processes by influencing chromatin
remodeling; forms at least two different complexes which
have distinct functions and members; Rpd3(L) recruitment
to the subtelomeric region is regulated by interaction
with the Hmt1p methyltransferase; delays late firing of
single copy origins by opposing the Fkh1/2 origin
activation pathway"
/codon_start=1
/product="histone deacetylase RPD3"
/protein_id="NP_014069.1"
/db_xref="GeneID:855386"
/db_xref="SGD:S000005274"
/translation="MVYEATPFDPITVKPSDKRRVAYFYDADVGNYAYGAGHPMKPHR
IRMAHSLIMNYGLYKKMEIYRAKPATKQEMCQFHTDEYIDFLSRVTPDNLEMFKRESV
KFNVGDDCPVFDGLYEYCSISGGGSMEGAARLNRGKCDVAVNYAGGLHHAKKSEASGF
CYLNDIVLGIIELLRYHPRVLYIDIDVHHGDGVEEAFYTTDRVMTCSFHKYGEFFPGT
GELRDIGVGAGKNYAVNVPLRDGIDDATYRSVFEPVIKKIMEWYQPSAVVLQCGGDSL
SGDRLGCFNLSMEGHANCVNYVKSFGIPMMVVGGGGYTMRNVARTWCFETGLLNNVVL
DKDLPYNEYYEYYGPDYKLSVRPSNMFNVNTPEYLDKVMTNIFANLENTKYAPSVQLN
HTPRDAEDLGDVEEDSAEAKDTKGGSQYARDLHVEHDNEFY"
gene complement(<19541..>22633)
/gene="PEX6"
/locus_tag="YNL329C"
/gene_synonym="PAS8"
/db_xref="GeneID:855387"
mRNA complement(<19541..>22633)
/gene="PEX6"
/locus_tag="YNL329C"
/gene_synonym="PAS8"
/product="AAA family ATPase peroxin 6"
/transcript_id="NM_001183167.1"
/db_xref="GeneID:855387"
CDS complement(19541..22633)
/gene="PEX6"
/locus_tag="YNL329C"
/gene_synonym="PAS8"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:12808025]"
/experiment="EXISTENCE:direct assay:GO:0005778 peroxisomal
membrane [PMID:16911527]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:12808025]"
/experiment="EXISTENCE:direct assay:GO:0016558 protein
import into peroxisome matrix [PMID:29321502]"
/experiment="EXISTENCE:direct assay:GO:0016562 protein
import into peroxisome matrix, receptor recycling
[PMID:16007078]"
/experiment="EXISTENCE:direct assay:GO:0043335 protein
unfolding [PMID:16007078|PMID:29321502]"
/experiment="EXISTENCE:direct assay:GO:0140318 protein
transporter activity [PMID:16007078]"
/experiment="EXISTENCE:direct assay:GO:1904949 ATPase
complex [PMID:16007078]"
/experiment="EXISTENCE:genetic interaction:GO:0016562
protein import into peroxisome matrix, receptor recycling
[PMID:16007078]"
/experiment="EXISTENCE:mutant phenotype:GO:0005777
peroxisome [PMID:12808025]"
/experiment="EXISTENCE:mutant phenotype:GO:0016562 protein
import into peroxisome matrix, receptor recycling
[PMID:16007078]"
/experiment="EXISTENCE:mutant phenotype:GO:0016887 ATP
hydrolysis activity [PMID:15634331]"
/note="AAA-peroxin; heterodimerizes with AAA-peroxin Pex1p
and participates in the recycling of peroxisomal signal
receptor Pex5p from the peroxisomal membrane to the
cystosol; mutations in human PEX6 can lead to severe
peroxisomal disorders and early death"
/codon_start=1
/product="AAA family ATPase peroxin 6"
/protein_id="NP_014070.1"
/db_xref="GeneID:855387"
/db_xref="SGD:S000005273"
/translation="MKASLTFSLSGIYAPCSISRDIYLEYGDKKAECLYGTIRLPQYG
PGCTPGKIVHCVLDDSLPFCSIVVPSKLFGFMPTQPTMDFCYFEPILDNVVPVLDSVT
FLINEQLYSKLMDLPQEMQQIQFLHYKYNINSMETVVHSRDILTSGLCQILNCSPFPQ
GLVDFTETQLILVNDTEQKLSALKYANEDEEYALPKIGTNSALSIDLESLPCTISRDL
LRPAPHINDDNSIYAFTDAETLLRLDVTSGSFITVSNMGCVRLVKLFVLLLPNGFKKR
TIYAPPKIIASFPDCSVVTISKSNIGHTDIPIANQVFISRVGGWLQSQKCFQNIILTT
LKKFFSESKRILCQNDLIPIAFDSSMADLNIAEENDESDDEDELGQYYKNDSLVWFFV
TSAELDCFSKDNSHFIIDPNRTKLITTNITNRRPLPLSRSNLQRYYGFAETFYYDLHI
FPYVRQLVNILETSFNCSQRGITLNASVLLHSTTNNVGKATMVRFASKYLGIHLLEID
CLSLTSNSRQLDSTSKIIGYIRAKCENVLPYASPAVIFLAHLDSILLDVNANQDPEAI
KLQKSINFEMSKLLDDFTFKFPGTTFVGSVNNIDNVPSSFRSHMRFEILVPVPSEAQR
LRIFQWYLSSHELNRDVQQKVPVSYMDNISFSSLSSYSAGLTPLDIKSIVETARMTAT
ARFYQESKKCGWLPQSILITQEDLSKATSKARNEFSVSIGAPQIPNVTWDDIGGIDFV
KGEILDTIDMPLKHPELFTSGMKKRSGILFYGPPGTGKTLMAKAIATNFSLNFFSVKG
PELLNMYIGESEANVRRVFQKAREAKPCVIFFDEIDSVAPKRGNQGDSGGVMDRIVSQ
LLAELDGMSTDADGVFVIGATNRPDLLDEALLRPGRFDKLLYLGIPDTDTKQLNILEA
LTRKFVLDNDVKLIELAKLCPFNYTGADFYALCSDAMLNAMSRIARMVEKKVSQHNEL
TGENISTRRWFDKIATKEDTKVVVKMEDFLKAQEQLTPSVSRAELNHYEAVRANFEGA
"
gene complement(<22834..>23274)
/gene="MDJ2"
/locus_tag="YNL328C"
/db_xref="GeneID:855388"
mRNA complement(<22834..>23274)
/gene="MDJ2"
/locus_tag="YNL328C"
/product="Mdj2p"
/transcript_id="NM_001183166.1"
/db_xref="GeneID:855388"
CDS complement(22834..23274)
/gene="MDJ2"
/locus_tag="YNL328C"
/experiment="EXISTENCE:direct assay:GO:0001671 ATPase
activator activity [PMID:16027163]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:9325105]"
/experiment="EXISTENCE:genetic interaction:GO:0030150
protein import into mitochondrial matrix [PMID:16027163]"
/experiment="EXISTENCE:physical interaction:GO:0001405 PAM
complex, Tim23 associated import motor [PMID:16027163]"
/experiment="EXISTENCE:physical interaction:GO:0030150
protein import into mitochondrial matrix [PMID:16027163]"
/note="Constituent of the mitochondrial import motor;
associated with the presequence translocase; function
overlaps with that of Pam18p; stimulates the ATPase
activity of Ssc1p to drive mitochondrial import; contains
a J domain"
/codon_start=1
/product="Mdj2p"
/protein_id="NP_014071.1"
/db_xref="GeneID:855388"
/db_xref="SGD:S000005272"
/translation="MVLPIIIGLGVTMVALSVKSGLNAWTVYKTLSPLTIAKLNNIRI
ENPTAGYRDALKFKSSLIDEELKNRLNQYQGGFAPRMTEPEALLILDISAREINHLDE
KLLKKKHRKAMVRNHPDRGGSPYMAAKINEAKEVLERSVLLRKR"
gene <24048..>27173
/gene="EGT2"
/locus_tag="YNL327W"
/db_xref="GeneID:855389"
mRNA <24048..>27173
/gene="EGT2"
/locus_tag="YNL327W"
/product="Egt2p"
/transcript_id="NM_001183165.2"
/db_xref="GeneID:855389"
CDS 24048..27173
/gene="EGT2"
/locus_tag="YNL327W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005621 cellular
bud scar [PMID:27229769]"
/experiment="EXISTENCE:direct assay:GO:0005933 cellular
bud [PMID:13679573]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:10383953]"
/experiment="EXISTENCE:direct assay:GO:0030428 cell septum
[PMID:15452134]"
/experiment="EXISTENCE:mutant phenotype:GO:0000920 septum
digestion after cytokinesis [PMID:8668141]"
/note="Glycosylphosphatidylinositol (GPI)-anchored cell
wall endoglucanase; localizes in birth scars; required for
proper cell separation after cytokinesis; expression is
activated by Swi5p and tightly regulated in a cell
cycle-dependent manner"
/codon_start=1
/product="Egt2p"
/protein_id="NP_014072.2"
/db_xref="GeneID:855389"
/db_xref="SGD:S000005271"
/translation="MNKLLLHLVRVISILGLANALTQTQPILKDIQITDSYTKTKECT
DPDHWFIIEGQLSIPKGSQQNITFQVPDAFSSFPQEPFSIKHNSNSVATISRPDKSTN
NFTISIPEKSSEDITTTFNFLAQLTSDAKSKVTEPKSIVYSFYSENTMFNDVIDYVAK
NTSAITTDGGIYKTNNTAWFTVDLPMRTFRNPVYLTSQTSSSSDYVFDTSLTKFEVVT
AVDSFNEPINAIPYTTVHDYSTEDEIRCLFNSTISGGLYFRVTYFTKKLSTSSISNTV
ELTYPDEGTSVRLLGKRDTSTTLASELYSESAANIDSTTSDDTTSSDAAITPTYSNST
LSSYTSQSSAIPEVAVTASLSSGILSSTVDGASTSADASMSAVSTVSSSSEQASSSSI
SLSAPSSSNSTFTTPSSSLSATETYSIISSASISVTQASYIDNSTTTAVTQSTSTIAV
SSAEKLSSTLSYTSNVTISVSSATQHTTTPSYVSNSTTLSSSSVLESVISSPYLANTT
VSGASSASQSTNPPYVSNSTTSSATQLATIAPFAINITGTSISSSITNTSSVSSTTSS
LSSGPFVSNTTVASGSYILTTTTESAQLTEIGSLIPISTITTSTTTSGTDKTGSNKVA
SSTEIAQSIVNNSSLSVSTINTNAATAAANARNATFTHATHSGSLQPSYHSSSLLSST
IDTKVTTATTSTSRDGSSSLAFTTGLNQSVVTGTDKSDTYSVISSTESAQVTEYDSLL
PISTLKPTVVTGTSRNSTFSMVSSTKLTEATATDKGDAYSVISSTQSAQVTEYGSMLP
ISTLETPTVIMSTDESGYFTLTTCTESGQATEYGSLIPISTLDGSVIYTFTGESVVVG
YSTTVGAAQYAQHTSLVPVSTIKGSKTSLSTEESVVAGYSTTVGAAQYAQHTSLVPVS
TIKGSKTSLSTEESVVAGYSTTVDSAQYAEHTNLVAIDTLKTSTFQKATATEVCVTCT
ALSSPHSATLDAGTTISLPTSSSTSLSTIITWYSSSTIKPPSISTYSGAAGQLTIRIG
SLLLGLISFLL"
gene complement(<27337..>28347)
/gene="PFA3"
/locus_tag="YNL326C"
/db_xref="GeneID:855390"
mRNA complement(<27337..>28347)
/gene="PFA3"
/locus_tag="YNL326C"
/product="palmitoyltransferase PFA3"
/transcript_id="NM_001183164.1"
/db_xref="GeneID:855390"
CDS complement(27337..28347)
/gene="PFA3"
/locus_tag="YNL326C"
/EC_number="2.3.1.225"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:16301533|PMID:14562095|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:16186255]"
/experiment="EXISTENCE:direct assay:GO:0016409
palmitoyltransferase activity [PMID:16186255]"
/experiment="EXISTENCE:direct assay:GO:0018345 protein
palmitoylation [PMID:16186255]"
/experiment="EXISTENCE:mutant phenotype:GO:0000324
fungal-type vacuole [PMID:16301533]"
/experiment="EXISTENCE:mutant phenotype:GO:0016409
palmitoyltransferase activity [PMID:26224664]"
/experiment="EXISTENCE:mutant phenotype:GO:0042144 vacuole
fusion, non-autophagic [PMID:16301533|PMID:16186255]"
/note="Palmitoyltransferase for Vac8p; required for
vacuolar membrane fusion; contains an
Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain;
autoacylates; required for vacuolar integrity under stress
conditions"
/codon_start=1
/product="palmitoyltransferase PFA3"
/protein_id="NP_014073.1"
/db_xref="GeneID:855390"
/db_xref="SGD:S000005270"
/translation="MNDRLSLTSLFPRCLTTCLYIWTAYITLTRIHQIPRWFLALTIV
PTLAVALYTYYKVIARGPGSPLDFPDLLVHDLKAAENGLELPPEYMSKRCLTLKHDGR
FRVCQVCHVWKPDRCHHCSSCDVCILKMDHHCPWFAECTGFRNQKFFIQFLMYTTLYA
FLVLIYTCYELGTWFNSGSFNRELIDFHLLGVALLAVAVFISVLAFTCFSIYQVCKNQ
TTIEVHGMRRYRRDLEILNDSYGTNEHLENIFDLGSSMANWQDIMGTSWLEWILPIET
FKYKKSKHTKDEKGLYFNVRPQVQDRLLSSRCLEDQLLRRVTPRPSLEADRASVEIID
AN"
rep_origin 28469..28700
/note="ARS1405; Autonomously Replicating Sequence"
/db_xref="SGD:S000118382"
gene complement(<28739..>31378)
/gene="FIG4"
/locus_tag="YNL325C"
/db_xref="GeneID:855392"
mRNA complement(<28739..>31378)
/gene="FIG4"
/locus_tag="YNL325C"
/product="phosphatidylinositol-3,5-bisphosphate
5-phosphatase"
/transcript_id="NM_001183163.1"
/db_xref="GeneID:855392"
CDS complement(28739..31378)
/gene="FIG4"
/locus_tag="YNL325C"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:14528018]"
/experiment="EXISTENCE:direct assay:GO:0034399 nuclear
periphery [PMID:25817432]"
/experiment="EXISTENCE:direct assay:GO:0043813
phosphatidylinositol-3,5-bisphosphate 5-phosphatase
activity [PMID:14528018]"
/experiment="EXISTENCE:direct assay:GO:0046856
phosphatidylinositol dephosphorylation [PMID:14528018]"
/experiment="EXISTENCE:direct assay:GO:0070772 PAS complex
[PMID:19037259]"
/experiment="EXISTENCE:genetic interaction:GO:0046856
phosphatidylinositol dephosphorylation [PMID:11950935]"
/experiment="EXISTENCE:physical interaction:GO:0070772 PAS
complex [PMID:19037259]"
/note="Phosphatidylinositol 3,5-bisphosphate
(PtdIns[3,5]P2) phosphatase; required for efficient mating
and response to osmotic shock; physically associates with
and regulated by Vac14p; contains a SAC1-like domain;
homologous to human FIG4, which is associated with CMT4J,
a form of Charcot-Marie-Tooth disorder"
/codon_start=1
/product="phosphatidylinositol-3,5-bisphosphate
5-phosphatase"
/protein_id="NP_014074.1"
/db_xref="GeneID:855392"
/db_xref="SGD:S000005269"
/translation="MNNDAMEHTLGGGILTTSGSKQRKTSKFVMGKYTLYETKDRMYI
VGSNKRETMFRILEIDLTVPRGELTVLEDNVFFTRNEIMNVLASLEEATEDGLHKKIT
GYGLLGFIKFTCWYYLIMVTKYSQVAVIGGHGIYHIDGIDIIPITNNYKKPEKSSDEA
RLLNIFKDLDLTKTFYFSYTYDITNTLQTNILREKLKAVDRCDITIPCGITDYNEMFV
WNNNLLSPIFACIDTVFDWFQCIIHGFIDQVNVSVLGKSIYITLIARRSHHFAGARFL
KRGVNNKGHVANEVETEQIVTDMILTPFHQPGNGFFDSDRYTSFVQHRGSIPLYWTQD
ASNLTTKPPIRINVVDPFFSPAALHFDNLFQRYGGGTIQILNLIKTKEKTPRETKLLW
EFEQCIDYLNEFLPTLKKLDYTSWDMSRASKQDGQGVIEFLEKYAVNTVTTTGIFHNG
PDFASTKIQEGICRSNCIDCLDRTNAAQFVIGKRALGCQLKSLGIIDNSYLEYDSDIV
NILTELFHDLGDTIALQYGGSHLVNTMETYRKINQWSSHSRDMIESIKRFYSNSFVDA
QRQDAINLFLGHYSWREGFPSLWEMNTDFYLHNAYSLNMPKRSYIHWWNDYNIKSVKE
LINEELIATGNDVTREKIIKNVRGYPGAFDNYWNEYYLPRSVTWIRDLFAYNMNSTRR
YHNALSKQDKAMSPFTSRKQSWLNNKLKMITSSKSLEKAEGRVVETTDLDRDTSPKQE
LELYEHYLHIISDRSQKLEEKMNSFSYSKYPIFISHESSEIPPMRKVIGEPLVDIAED
FTDVYDDDDDGDDENDEMTTEALLIAPDHVSVDEKFYEKVLNVDDYKPALDDYSAVIH
IKPDNLQLYRDLCFSKDIQLDFQ"
gene <31944..>33188
/gene="LEM3"
/locus_tag="YNL323W"
/gene_synonym="BRE3; ROS3"
/db_xref="GeneID:855393"
mRNA <31944..>33188
/gene="LEM3"
/locus_tag="YNL323W"
/gene_synonym="BRE3; ROS3"
/product="Lem3p"
/transcript_id="NM_001183161.1"
/db_xref="GeneID:855393"
CDS 31944..33188
/gene="LEM3"
/locus_tag="YNL323W"
/gene_synonym="BRE3; ROS3"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762|PMID:12133835]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:12133835]"
/experiment="EXISTENCE:genetic interaction:GO:0044088
regulation of vacuole organization [PMID:25359886]"
/experiment="EXISTENCE:mutant phenotype:GO:0007166 cell
surface receptor signaling pathway [PMID:11063677]"
/experiment="EXISTENCE:mutant phenotype:GO:0015247
aminophospholipid flippase activity [PMID:12133835]"
/experiment="EXISTENCE:mutant phenotype:GO:0045332
phospholipid translocation [PMID:12842877]"
/experiment="EXISTENCE:mutant phenotype:GO:0140345
phosphatidylcholine flippase activity [PMID:12133835]"
/experiment="EXISTENCE:physical interaction:GO:1990531
phospholipid-translocating ATPase complex [PMID:15090616]"
/note="Membrane protein of the plasma membrane and ER;
interacts specifically in vivo with the phospholipid
translocase (flippase) Dnf1p; involved in translocation of
phospholipids and alkylphosphocholine drugs across the
plasma membrane; null mutant requires tryptophan due to
mislocalization of tryptophan permease Tat2p"
/codon_start=1
/product="Lem3p"
/protein_id="NP_014076.1"
/db_xref="GeneID:855393"
/db_xref="SGD:S000005267"
/translation="MVNFDLGQVGEVFRRKDKGAIVSGDNPEEEEDVDASEFEEDEVK
PVRTKNRRPKEDAFTQQRLAAINPVLTPRTVLPLYLLIAVVFVIVGGCILAQNSKVDE
VTIYYQDCMTNATSSWSDIPSEHWQFVFHKYKTYNTAPQWRFVDDESDDFTKQRGTCQ
IRFTTPSDMKNNVYLNYVLEKFAANHRRYVLSFSEDQIRGEDASYETVHDATGINCKP
LSKNADGKIYYPCGLIANSMFNDTFPLQLTNVGDTSNNYSLTNKGINWESDKKRYKKT
KYNYTQIAPPPYWEKMYPDGYNETNIPDIQDWEEFQNWMRPGAFDKITKLIRINKNDT
LPAGEYQLDIGLHWPVLEFNGKKGIYLTHGSHLGGRNPFLGIVYLIGGCICAAMALIL
LTFWLFGGRKIADASSLSWNMK"
gene complement(<33294..>34235)
/gene="KRE1"
/locus_tag="YNL322C"
/db_xref="GeneID:855394"
mRNA complement(<33294..>34235)
/gene="KRE1"
/locus_tag="YNL322C"
/product="Kre1p"
/transcript_id="NM_001183160.1"
/db_xref="GeneID:855394"
CDS complement(33294..34235)
/gene="KRE1"
/locus_tag="YNL322C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:11956747]"
/note="Cell wall glycoprotein involved in beta-glucan
assembly; serves as a K1 killer toxin membrane receptor"
/codon_start=1
/product="Kre1p"
/protein_id="NP_014077.1"
/db_xref="GeneID:855394"
/db_xref="SGD:S000005266"
/translation="MMRRTLLHSFATLLLSLSLWSAAVMAAVTTQVTVVTNVAGALVT
ETTIWDPATAAAAATTTAQTGFFTTVFTTTNDVGTTVTLTQTVNRATMLPTTTTSTSS
TGKTTTTVPTATSSLSSGLYLSTVTTTNDLGTTVTLTQTFTHSSTSATSSASSSVSSS
VSSSGSSSSVKTTTSTGSAVAETGTRPDPSTDFTEPPVSAVTSLSIDSYITITEGTTS
TYTTTRAPTSMWVTVVRQGNTITVQTTFVQRFSSQYVTVASPSVGSIGMGTLTGTVGV
IKSAIKKTVSHNEAQHLGMSSFTSILGGLLTVLIWFL"
gene <34696..>37422
/gene="VNX1"
/locus_tag="YNL321W"
/db_xref="GeneID:855395"
mRNA <34696..>37422
/gene="VNX1"
/locus_tag="YNL321W"
/product="calcium/hydrogen antiporter"
/transcript_id="NM_001183159.1"
/db_xref="GeneID:855395"
CDS 34696..37422
/gene="VNX1"
/locus_tag="YNL321W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:17588950]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762|PMID:20709757]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:20578725]"
/experiment="EXISTENCE:direct assay:GO:1990816
vacuole-mitochondrion membrane contact site
[PMID:25026036]"
/experiment="EXISTENCE:mutant phenotype:GO:0006813
potassium ion transport [PMID:17588950]"
/experiment="EXISTENCE:mutant phenotype:GO:0006814 sodium
ion transport [PMID:17588950]"
/experiment="EXISTENCE:mutant phenotype:GO:0015369
calcium:proton antiporter activity [PMID:20578725]"
/experiment="EXISTENCE:mutant phenotype:GO:0015385
sodium:proton antiporter activity [PMID:17588950]"
/experiment="EXISTENCE:mutant phenotype:GO:0015386
potassium:proton antiporter activity [PMID:17588950]"
/experiment="EXISTENCE:mutant phenotype:GO:1902600 proton
transmembrane transport [PMID:20578725]"
/note="Calcium/H+ antiporter localized to endoplasmic
reticulum membrane; member of calcium exchanger (CAX)
family; targeted to vacuole via AP-3 pathway"
/codon_start=1
/product="calcium/hydrogen antiporter"
/protein_id="NP_014078.1"
/db_xref="GeneID:855395"
/db_xref="SGD:S000005265"
/translation="MAKNNHISASGNSTSGDHRLKEEVLTPTTSASTPHRIFSVDDDP
KEIQNDIRYLEGLHEGLKFALHANKSKRSVSSQSPIVHSSNNTLHHHEHQQHLPPTLE
SLSSKSHSVPDLNTATPSSPKRMHSSIRELPHDDNDDEDANDDSRFIIHDSHGHDLLI
DEINCQSPSHLENNDQASNASSTESFTLRERQDAINETHPFGIRIWKPALYKKHRSVQ
RTAAQDIHETQLKTITWEVTCSNVLWFILFGFPIAILFYSAAIVVFLLGGGGLVTNSA
KEYSKCLYKLANYFLWPFGKMVYLLQDEQYLQEDKDEGISMQQFYNWVTSYSNRLVFH
QSQAKFQQREDHPAPATESSSLMPPANTTATPLNSNHPSYNSIRHEIPHAAAQRRYFG
RGKWSWGRVLFYTIFHLVLQPILAVLSLCLWLLVFTIPMSNVLWQIMYHCRRHPLALG
FKYVENSSQSHENEITQQQLNKNILLCTFRAAGWHYYKYTVDGTNVIVVNLISIVFFT
IFDFYVLKNFLHWKTWFTYESSIFILCLTSTIPLAFYIGQAVASISAQTSMGVGAVIN
AFFSTIVEIFLYCVALQQKKGLLVEGSMIGSILGAVLLLPGLSMCGGALNRKTQRYNP
ASAGVSSALLIFSMIVMFVPTVLYEIYGGYSVNCADGANDRDCTFSHPPLKFNRLFTH
VIQPMSISCAIVLFCAYIIGLWFTLRTHAKMIWQLPIADPTSTAPEQQEQNSHDAPNW
SRSKSTCILLMSTLLYAIIAEILVSCVDAVLEDIPSLNPKFLGLTIFALIPNTTEFLN
AISFAIHGNVALSMEIGSAYALQVCLLQIPSLVIYSIFYTWNVKKSMINIRTQMFPLV
FPRWDIFGAMTSVFMFTYLYAEGKSNYFKGSMLILLYIIIVVGFYFQGALSE"
gene <37700..>38554
/locus_tag="YNL320W"
/db_xref="GeneID:855396"
mRNA <37700..>38554
/locus_tag="YNL320W"
/product="uncharacterized protein"
/transcript_id="NM_001183158.1"
/db_xref="GeneID:855396"
CDS 37700..38554
/locus_tag="YNL320W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/note="hypothetical protein; the authentic, non-tagged
protein is detected in highly purified mitochondria in
high-throughput studies"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014079.1"
/db_xref="GeneID:855396"
/db_xref="SGD:S000005264"
/translation="MLWKVSKMFLGGLVALTTISVATLYHYQNRLVYPSWAQGARNHV
DTPDSRGIPYEKLTLITQDHIKLEAWDIKNENSTSTVLILCPNAGNIGYFILIIDIFY
RQFGMSVFIYSYRGYGNSEGSPSEKGLKLDADCVISHLSTDSFHSKRKLVLYGRSLGG
ANALYIASKFRDLCDGVILENTFLSIRKVIPYIFPLLKRFTLLCHEIWNSEGLMGSCS
SETPFLFLSGLKDEIVPPFHMRKLYETCPSSNKKIFEFPLGSHNDTIIQDGYWDIIRD
FLIEKGFI"
gene complement(<38707..>40329)
/gene="HXT14"
/locus_tag="YNL318C"
/db_xref="GeneID:855398"
mRNA complement(<38707..>40329)
/gene="HXT14"
/locus_tag="YNL318C"
/product="Hxt14p"
/transcript_id="NM_001183156.1"
/db_xref="GeneID:855398"
CDS complement(38707..40329)
/gene="HXT14"
/locus_tag="YNL318C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0005354
galactose transmembrane transporter activity
[PMID:10618490]"
/experiment="EXISTENCE:mutant phenotype:GO:0008645 hexose
transmembrane transport [PMID:10618490]"
/note="Protein with similarity to hexose transporter
family members; expression is induced in low glucose and
repressed in high glucose; the authentic, non-tagged
protein is detected in highly purified mitochondria in
high-throughput studies"
/codon_start=1
/product="Hxt14p"
/protein_id="NP_014081.1"
/db_xref="GeneID:855398"
/db_xref="SGD:S000005262"
/translation="MTAQIPYQHSSGYISHFHNNELDAGRGRDYNVTIKYLDDKEENI
EGQAAKISHNASLHIPVLLCLVISLGGFIFGWDIGTIGGMTNMVSFQEKFGTTNIIHD
DETIFVSTKKLTDLQIGLIISIFNISCGVGALTLSKIGDWIGRKGGIWFALVVYCIGI
TIQILSYGRWYFLTLGRAVTGIGVGVTTVLVPMFLSENSPLKIRGSMVSTYQLIVTFG
ILMGNILNFICERCYKDPTQNIAWQLPLFLGYIWAIIIGMSLVYVPESPQYLAKIKND
VPSAKYSFARMNGIPATDSMVIEFIDDLLENNYNNEETNNESKKQSLVKRNTFEFIMG
KPKLWLRLIIGMMIMAFQQLSGINYFFYYGTSVFKGVGIKDPYITSIILSSVNFLSTI
LGIYYVEKWGHKTCLLYGSTNLLFYMMTYATVGTFGRETDFSNIVLIIVTCCFIFWFA
ITLGPVTFVLVSELFPLRTRAISMAICTFINWMFNFLISLLTPMIVSKIDFKLGYIFA
ACLLALIIFSWILVPETRKKNEQEINKIFEPE"
gene <40619..>42016
/gene="PFS2"
/locus_tag="YNL317W"
/db_xref="GeneID:855399"
mRNA <40619..>42016
/gene="PFS2"
/locus_tag="YNL317W"
/product="cleavage polyadenylation factor subunit PFS2"
/transcript_id="NM_001183155.1"
/db_xref="GeneID:855399"
CDS 40619..42016
/gene="PFS2"
/locus_tag="YNL317W"
/experiment="EXISTENCE:curator inference:GO:0003723 RNA
binding [PMID:12819204]"
/experiment="EXISTENCE:direct assay:GO:0005847 mRNA
cleavage and polyadenylation specificity factor complex
[PMID:12819204]"
/experiment="EXISTENCE:direct assay:GO:0006397 mRNA
processing [PMID:11344258]"
/experiment="EXISTENCE:mutant phenotype:GO:0006397 mRNA
processing [PMID:10619842]"
/note="Integral subunit of the pre-mRNA CPF complex; the
cleavage and polyadenylation factor (CPF) complex plays an
essential role in mRNA 3'-end formation by bridging
different processing factors and thereby promoting the
assembly of the processing complex"
/codon_start=1
/product="cleavage polyadenylation factor subunit PFS2"
/protein_id="NP_014082.1"
/db_xref="GeneID:855399"
/db_xref="SGD:S000005261"
/translation="MDGHNQNQYQNQNQIQQSQQPPLKKYVTQRRSVDVSSPYINLYY
NRRHGLPNLVVEPETSYTIDIMPPNAYRGRDRVINLPSKFTHLSSNKVKHVIPAIQWT
PEGRRLVVATYSGEFSLWNASSFTFETLMQAHDSAVTTMKYSHDSDWMISGDADGMIK
IWQPNFSMVKEIDAAHTESIRDMAFSSNDSKFVTCSDDNILKIWNFSNGKQERVLSGH
HWDVKSCDWHPEMGLIASASKDNLVKLWDPRSGNCISSILKFKHTVLKTRFQPTKGNL
LMAISKDKSCRVFDIRYSMKELMCVRDETDYMTLEWHPINESMFTLACYDGSLKHFDL
LQNLNEPILTIPYAHDKCITSLSYNPVGHIFATAAKDRTIRFWTRARPIDPNAYDDPT
YNNKKINGWFFGINNDINAVREKSEFGAAPPPPATLEPHALPNMNGFINKKPRQEIPG
IDSNIKSSTLPGLSI"
gene complement(<42071..>43075)
/gene="PHA2"
/locus_tag="YNL316C"
/db_xref="GeneID:855400"
mRNA complement(<42071..>43075)
/gene="PHA2"
/locus_tag="YNL316C"
/product="prephenate dehydratase PHA2"
/transcript_id="NM_001183154.1"
/db_xref="GeneID:855400"
CDS complement(42071..43075)
/gene="PHA2"
/locus_tag="YNL316C"
/EC_number="4.2.1.51"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0004664
prephenate dehydratase activity [PMID:1532796]"
/experiment="EXISTENCE:mutant phenotype:GO:0009094
L-phenylalanine biosynthetic process [PMID:1532796]"
/note="Prephenate dehydratase; catalyzes the conversion of
prephanate to phenylpyruvate, which is a step in the
phenylalanine biosynthesis pathway"
/codon_start=1
/product="prephenate dehydratase PHA2"
/protein_id="NP_014083.2"
/db_xref="GeneID:855400"
/db_xref="SGD:S000005260"
/translation="MASKTLRVLFLGPKGTYSHQAALQQFQSTSDVEYLPAASIPQCF
NQLENDTSIDYSVVPLENSTNGQVVFSYDLLRDRMIKKALSLPAPADTNRITPDIEVI
AEQYVPITHCLISPIQLPNGIASLGNFEEVIIHSHPQVWGQVECYLRSMAEKFPQVTF
IRLDCSSTSESVNQCIRSSTADCDNILHLAIASETAAQLHKAYIIEHSINDKLGNTTR
FLVLKRRENAGDNEVEDTGLLRVNLLTFTTRQDDPGSLVDVLNILKIHSLNMCSINSR
PFHLDEHDRNWRYLFFIEYYTEKNTPKNKEKFYEDISDKSKQWCLWGTFPRNERYYHK
"
gene complement(<43324..>44280)
/gene="ATP11"
/locus_tag="YNL315C"
/db_xref="GeneID:855401"
mRNA complement(<43324..>44280)
/gene="ATP11"
/locus_tag="YNL315C"
/product="Atp11p"
/transcript_id="NM_001183153.1"
/db_xref="GeneID:855401"
CDS complement(43324..44280)
/gene="ATP11"
/locus_tag="YNL315C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961|PMID:1532796]"
/experiment="EXISTENCE:direct assay:GO:0051082 unfolded
protein binding [PMID:10681564|PMID:12829692]"
/experiment="EXISTENCE:mutant phenotype:GO:0007005
mitochondrion organization [PMID:2142305]"
/experiment="EXISTENCE:mutant phenotype:GO:0033615
mitochondrial proton-transporting ATP synthase complex
assembly [PMID:2142305]"
/experiment="EXISTENCE:physical interaction:GO:0033615
mitochondrial proton-transporting ATP synthase complex
assembly [PMID:10681564]"
/note="Molecular chaperone; required for the assembly of
alpha and beta subunits into the F1 sector of
mitochondrial F1F0 ATP synthase; N-terminally
propionylated in vivo"
/codon_start=1
/product="Atp11p"
/protein_id="NP_014084.1"
/db_xref="GeneID:855401"
/db_xref="SGD:S000005259"
/translation="MWRLTRKIGTRIHISNQLSPIFNKAIGTVPVFRFYSSSPEQKYR
KKLLEEAQKQGFNSIEELKNHLKETIESKKREFNKIDPLKELEDYQQKTQMENNNSKH
LMTKSRSPLDPSAPKVPFKTLDSFLDVGKLKDLSKQEVEFLWRARWAQKDNTLCAVIP
VSVYDKMMANARNNPIFVLPLPRQVQSEDAKPNEEQGMELHYIQWQFVGPQTTHCMMT
SLAEYKLHQEFARPHTTLQFHSDLVKDKGIVFMNGHVEPDTNVNVQDAQLLLLNVQRF
YGAMGEETPVAKQRVQLLRDFSKASPGFTVEKLISLSQSMEN"
gene <44447..>45214
/gene="DAL82"
/locus_tag="YNL314W"
/db_xref="GeneID:855402"
mRNA <44447..>45214
/gene="DAL82"
/locus_tag="YNL314W"
/product="Dal82p"
/transcript_id="NM_001183152.1"
/db_xref="GeneID:855402"
CDS 44447..45214
/gene="DAL82"
/locus_tag="YNL314W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10702289]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:8367295]"
/experiment="EXISTENCE:mutant phenotype:GO:0000256
allantoin catabolic process [PMID:1898922]"
/experiment="EXISTENCE:mutant phenotype:GO:0043565
sequence-specific DNA binding [PMID:10702289]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:10906145]"
/experiment="EXISTENCE:physical interaction:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:10906145]"
/note="Positive regulator of allophanate inducible genes;
binds a dodecanucleotide sequence upstream of all genes
that are induced by allophanate; contains an UISALL
DNA-binding, a transcriptional activation, and a
coiled-coil domain"
/codon_start=1
/product="Dal82p"
/protein_id="NP_014085.1"
/db_xref="GeneID:855402"
/db_xref="SGD:S000005258"
/translation="MDESVDPVELLLRLLIRHKPHLKPYAYRQDSWQRVLDEYNRQTG
SRYRQSRTLKTKFRRLKDLFSADRAQFSPSQLKLMGALLDEAPEHPRPRTKFGNESSS
SLSSSSFIKSHPGPDPFQQLSSAEHPNNHSSDDEHSGSQPLPLDSITIGIPPTLHTIP
MILSKDNDVGKVIKSPKINKGTNRFSETVLPPQMAAEQSWSDSNMELEICLDYLHNEL
EVIKKRQEDFECKVLNKLNIIEALLSQMRPPSQGDKI"
gene complement(<45308..>48022)
/gene="EMW1"
/locus_tag="YNL313C"
/db_xref="GeneID:855403"
mRNA complement(<45308..>48022)
/gene="EMW1"
/locus_tag="YNL313C"
/product="tetratricopeptide repeat-containing protein
EMW1"
/transcript_id="NM_001183151.1"
/db_xref="GeneID:855403"
CDS complement(45308..48022)
/gene="EMW1"
/locus_tag="YNL313C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:21273246|PMID:14562095|PMID:14690591]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:21273246|PMID:14690591|PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0031505
fungal-type cell wall organization [PMID:21273246]"
/note="Essential conserved protein with a role in cell
wall integrity; contains six TPR (tetratricopeptide
repeat) domains clustered in the C-terminal region;
conditional mutant is suppressed by overexpression of
GFA1; protein abundance increases in response to DNA
replication stress"
/codon_start=1
/product="tetratricopeptide repeat-containing protein
EMW1"
/protein_id="NP_014086.1"
/db_xref="GeneID:855403"
/db_xref="SGD:S000005257"
/translation="METLLHAKLLLSAEVESLKSGSFDQTYVKKAEHIISGESYQLVQ
QFVDKFKGKISISGEISTSSVIAALNDFLNVEVFKMGQENEMLFLAIALLQTFIQNNY
TGPAARLKAISGLFGKTGIEIGAVNTALSRSLAIMGQPAYEFMDDPLYLVLSLLLLER
ITGQKSLFDVTPDQEIPLPIISAESTPGLLAVAYWWWARALLTQLSLIPEPSGFQASV
ASAIYQSADLAYAITKELPESIHEDFKRELCAMYYLENVKCSLAINTEHLCLPSLTRA
KKITNFEFVMTGARATRTKYQQKAHAGLIILAKSFTFQNFALRTTSATPETFALESDL
LLEKPHFESIADEPLDEQIYSKRQKVDLNEGYEEDKLLPLALRQENIPKLLLDLNPND
QPTLSDYDNIQLLLRLYTIKNTTPAKDPLVEEELTALLSRILYQNGDKNWSIFARSLW
ERSIIETTKAKTIERGLLQMQSLVEELDLKIKSKLVPSSSEINVASRLSYIHQLPFIP
RWQLDATLAEKYMSLGILKSAVEIYERLGMACETALCYAAVGDEKKAEEILLQRINEN
DSDARAYSILGDIKQDPSLWEKSWEIGKYVNAKNSLAKYYFNPPPKSGAQPNYSATLK
HLNDSLRQYPLSFETWYFYGCVGLQCGKMQIAAEAFTRCVSLDPYHALSWSNLSAAYT
KMDKLKEAYSCLKRAISCDAQKNWKIWENYMLVAVKLNEWEDVLTACKQLVSIRRDKS
GEGSIDLPIIEKLVELLVTSEYPEEPQQLSYFQKSCTEFICNTLPQVITTSARCWRLV
ARVELWRKRPWAALECHEKAYRAISHNPDLEVEEKVWNDTVDACEDLVAAYESLGEME
GKYGPGSLVCKDWKYKCRSTIKALMSKGKGRWDDSPGWDRLVEARSQI"
gene <48287..>49216
/gene="RFA2"
/locus_tag="YNL312W"
/gene_synonym="BUF1; RPA2; RPA32"
/db_xref="GeneID:855404"
mRNA join(<48287..48293,48402..>49216)
/gene="RFA2"
/locus_tag="YNL312W"
/gene_synonym="BUF1; RPA2; RPA32"
/product="Rfa2p"
/transcript_id="NM_001183150.1"
/db_xref="GeneID:855404"
CDS join(48287..48293,48402..49216)
/gene="RFA2"
/locus_tag="YNL312W"
/gene_synonym="BUF1; RPA2; RPA32"
/experiment="EXISTENCE:direct assay:GO:0000794 condensed
nuclear chromosome [PMID:9679065]"
/experiment="EXISTENCE:direct assay:GO:0003690
double-stranded DNA binding [PMID:7761422]"
/experiment="EXISTENCE:direct assay:GO:0003697
single-stranded DNA binding [PMID:8804316]"
/experiment="EXISTENCE:direct assay:GO:0005662 DNA
replication factor A complex [PMID:2554144]"
/experiment="EXISTENCE:direct assay:GO:0006260 DNA
replication [PMID:2554144]"
/experiment="EXISTENCE:direct assay:GO:0006265 DNA
topological change [PMID:22885009]"
/experiment="EXISTENCE:direct assay:GO:0006289
nucleotide-excision repair [PMID:8910442]"
/experiment="EXISTENCE:direct assay:GO:0030491
heteroduplex formation [PMID:12226081]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:7761422]"
/experiment="EXISTENCE:genetic interaction:GO:0000724
double-strand break repair via homologous recombination
[PMID:9679065]"
/experiment="EXISTENCE:mutant phenotype:GO:0000781
chromosome, telomeric region [PMID:14702040]"
/experiment="EXISTENCE:mutant phenotype:GO:0006260 DNA
replication [PMID:9093844]"
/experiment="EXISTENCE:mutant phenotype:GO:0006281 DNA
repair [PMID:9093844]"
/experiment="EXISTENCE:mutant phenotype:GO:0006289
nucleotide-excision repair [PMID:8910442]"
/experiment="EXISTENCE:mutant phenotype:GO:0007004
telomere maintenance via telomerase [PMID:14702040]"
/experiment="EXISTENCE:mutant phenotype:GO:0010833
telomere maintenance via telomere lengthening
[PMID:22354040]"
/experiment="EXISTENCE:mutant phenotype:GO:0045184
establishment of protein localization [PMID:14702040]"
/experiment="EXISTENCE:physical interaction:GO:0000722
telomere maintenance via recombination [PMID:2554144]"
/experiment="EXISTENCE:physical interaction:GO:0006312
mitotic recombination [PMID:2554144]"
/experiment="EXISTENCE:physical interaction:GO:0007131
reciprocal meiotic recombination [PMID:2554144]"
/experiment="EXISTENCE:physical interaction:GO:0016567
protein ubiquitination [PMID:18342608]"
/note="Subunit of heterotrimeric Replication Protein A
(RPA); RPA is a highly conserved single-stranded DNA
binding protein involved in DNA replication, repair, and
recombination; RPA protects against inappropriate telomere
recombination, and upon telomere uncapping, prevents cell
proliferation by a checkpoint-independent pathway; in
concert with Sgs1p-Top2p-Rmi1p, stimulates DNA
catenation/decatenation activity of Top3p; protein
abundance increases in response to DNA replication s"
/codon_start=1
/product="Rfa2p"
/protein_id="NP_014087.1"
/db_xref="GeneID:855404"
/db_xref="SGD:S000005256"
/translation="MATYQPYNEYSSVTGGGFENSESRPGSGESETNTRVNTLTPVTI
KQILESKQDIQDGPFVSHNQELHHVCFVGVVRNITDHTANIFLTIEDGTGQIEVRKWS
EDANDLAAGNDDSSGKGYGSQVAQQFEIGGYVKVFGALKEFGGKKNIQYAVIKPIDSF
NEVLTHHLEVIKCHSIASGMMKQPLESASNNNGQSLFVKDDNDTSSGSSPLQRILEFC
KKQCEGKDANSFAVPIPLISQSLNLDETTVRNCCTTLTDQGFIYPTFDDNNFFAL"
gene complement(<49397..>51688)
/gene="SKP2"
/locus_tag="YNL311C"
/db_xref="GeneID:855405"
mRNA complement(<49397..>51688)
/gene="SKP2"
/locus_tag="YNL311C"
/product="putative SCF ubiquitin ligase complex subunit
SKP2"
/transcript_id="NM_001183149.1"
/db_xref="GeneID:855405"
CDS complement(49397..51688)
/gene="SKP2"
/locus_tag="YNL311C"
/experiment="EXISTENCE:direct assay:GO:0019005 SCF
ubiquitin ligase complex [PMID:14747994]"
/experiment="EXISTENCE:mutant phenotype:GO:0030163 protein
catabolic process [PMID:20936605]"
/experiment="EXISTENCE:mutant phenotype:GO:1901494
regulation of cysteine metabolic process [PMID:20936605]"
/experiment="EXISTENCE:physical interaction:GO:0019005 SCF
ubiquitin ligase complex [PMID:14747994]"
/note="F-box hypothetical protein; predicted to be part of
an SCF ubiquitin protease complex; involved in regulating
protein levels of sulfur metabolism enzymes; may interact
with ribosomes, based on co-purification experiments"
/codon_start=1
/product="putative SCF ubiquitin ligase complex subunit
SKP2"
/protein_id="NP_014088.1"
/db_xref="GeneID:855405"
/db_xref="SGD:S000005255"
/translation="MKRLQLFGRSKYFSLVSSAAKEEEEEEEGCADAKSLLHSTSHDI
KSRSLRFNDKSSLMCLPTKVLLLILRTLDFNTLVTLCQVNSRFYNLITNEFLFQNVIL
DSKLSLLKFNALIHSEFHTSNIVTHSGDCSTQSRSQNARFLVRSIEFKNPQSQDSLLK
YSKFYNKSGQDSIIAGSYKLDSYDKDVKKLNNIRLNDETPIITSERIKLLDKLESNYF
HYTYIELMLDIIDYLPNLTRVILSDVEPNFKIPLWYSVFNDGSRDFFKKIIKGQQSIT
NEDLRTFQLSKKFVKEYESKYYSLPRLKILEIKANNKRQRTFNRQRHHQKLVLRPSLF
CCFGIINELKLENVTIDTESLDTPMEFLPLFLKNEDNELYSLQSPITALTLDSCDVVP
GNGILRLFHSYFKMVKHLSLLKINSKFDLLLCSCFPSLSNLTIDCNSKCFTNEQVVGE
SYYFQQRSLDTEDDFDDCNSMTETLFEAPSDSKIITPPPTSSVVLSLNLNYISRTTGN
DVSNNPSPDNNKKPAMLTAAQLQNFQRQRIPEFHSFYHYYRLLWERLPSKNISINVIN
IPFTNVYPLSPLSFWEHLARTITSVDETDEDVGDENDQETLIGYENNSIRDNIPNANA
VPNLSTVMSPESDIHHTYYWNNSVRRCLRDSLIKLKNRTIEYRDLDVEEFLQNVTLEN
FFNDFQDPENFKDIPNINLWCFLRNLSKFKAVKIRMLRHFSLCTPRTRYDWELLLKPV
LRVNVPIEVRDKDGFVLYSYGQK"
gene complement(<51907..>52431)
/gene="ZIM17"
/locus_tag="YNL310C"
/gene_synonym="HEP1; TIM15"
/db_xref="GeneID:855406"
mRNA complement(<51907..>52431)
/gene="ZIM17"
/locus_tag="YNL310C"
/gene_synonym="HEP1; TIM15"
/product="Zim17p"
/transcript_id="NM_001183148.1"
/db_xref="GeneID:855406"
CDS complement(51907..52431)
/gene="ZIM17"
/locus_tag="YNL310C"
/gene_synonym="HEP1; TIM15"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:16823961|PMID:14576278|PMID:14562095|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:15992824|PMID:15383543]"
/experiment="EXISTENCE:direct assay:GO:0051087
protein-folding chaperone binding [PMID:15719019]"
/experiment="EXISTENCE:mutant phenotype:GO:0006457 protein
folding [PMID:15719019]"
/experiment="EXISTENCE:mutant phenotype:GO:0006986
response to unfolded protein [PMID:15992824]"
/experiment="EXISTENCE:mutant phenotype:GO:0007005
mitochondrion organization [PMID:15719019]"
/experiment="EXISTENCE:mutant phenotype:GO:0030150 protein
import into mitochondrial matrix [PMID:15383543]"
/experiment="EXISTENCE:mutant phenotype:GO:0050821 protein
stabilization [PMID:16460754]"
/note="Protein co-chaperone with a zinc finger motif;
essential for protein import into mitochondria; may act
with Pam18p to facilitate recognition and folding of
imported proteins by Ssc1p (mtHSP70) in the mitochondrial
matrix; required for the maintenance of Ssc1p solubility
and assists in the functional interaction of Ssc1p with
substrate proteins"
/codon_start=1
/product="Zim17p"
/protein_id="NP_014089.2"
/db_xref="GeneID:855406"
/db_xref="SGD:S000005254"
/translation="MIPRTRTLLQSKIPITRYFARCWAPRVRYNVCRTLPAAALHTNI
IAHNEVKKDDKKVHLGSFKVDKPKMMIAFTCKKCNTRSSHTMSKQAYEKGTVLISCPH
CKVRHLIADHLKIFHDHHVTVEQLMKANGEQVSQDVGDLEFEDIPDSLKDVLGKYAKN
NSENASQLPHPSQK"
gene <52662..>53924
/gene="STB1"
/locus_tag="YNL309W"
/db_xref="GeneID:855407"
mRNA <52662..>53924
/gene="STB1"
/locus_tag="YNL309W"
/product="Stb1p"
/transcript_id="NM_001183147.1"
/db_xref="GeneID:855407"
CDS 52662..53924
/gene="STB1"
/locus_tag="YNL309W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11914276|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0030907 MBF
transcription complex [PMID:18794370]"
/experiment="EXISTENCE:direct assay:GO:0033309 SBF
transcription complex [PMID:18794370]"
/experiment="EXISTENCE:direct assay:GO:0070822 Sin3-type
complex [PMID:9393435]"
/experiment="EXISTENCE:genetic interaction:GO:0000082 G1/S
transition of mitotic cell cycle [PMID:10409718]"
/experiment="EXISTENCE:genetic interaction:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:10409718]"
/experiment="EXISTENCE:genetic interaction:GO:0031496
positive regulation of mating type switching
[PMID:9393435]"
/experiment="EXISTENCE:genetic interaction:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:9393435]"
/experiment="EXISTENCE:mutant phenotype:GO:0000082 G1/S
transition of mitotic cell cycle [PMID:18794370]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:18794370]"
/experiment="EXISTENCE:physical interaction:GO:0003713
transcription coactivator activity
[PMID:18794370|PMID:10409718|PMID:12832490]"
/experiment="EXISTENCE:physical interaction:GO:0070822
Sin3-type complex [PMID:9393435]"
/note="Protein with role in regulation of MBF-specific
transcription at Start; phosphorylated by Cln-Cdc28p
kinases in vitro; unphosphorylated form binds Swi6p, which
is required for Stb1p function; expression is cell-cycle
regulated; STB1 has a paralog, YOL131W, that arose from
the whole genome duplication"
/codon_start=1
/product="Stb1p"
/protein_id="NP_014090.2"
/db_xref="GeneID:855407"
/db_xref="SGD:S000005253"
/translation="MSQPQMSPEKEQELASKILHRAELAQMTRQLKLGLSNVPSTKRK
QDSTTKKRSGEDAEDVDEDHKTLLEAISPAKKPLHDDTNKMTVISPVKFVEKPNTPPS
SRQRKAEDRSQQIKPRKEDTPSTPRASATPIILPHASSHYQRPHDKNFMTPKRNNNNS
SNHSNNNNNIKKKAAGSKDAPQDSDNTAGADLLMYLATSPYNKSSHHGTPMAVRMPTT
PRSYHYASQLSLNGNTASTSNDAVRFSHIKPSASSPQSTFKSNLLPNFPDESLMDSPS
LYLSNNNGSVQATLSPQQRRKPTTNTLHPPSNVPTTPSRELNGTNFNLLRTPNFNMGD
YLHNLFSPSPRVPAQQGASNTSASIPSVPAMVPGSSSNTSAIATAAISSHTTNNFLDM
NANGIPLIVGPGTDRIGEGESIDDKLTD"
gene complement(<54122..>55897)
/gene="KRI1"
/locus_tag="YNL308C"
/db_xref="GeneID:855408"
mRNA complement(<54122..>55897)
/gene="KRI1"
/locus_tag="YNL308C"
/product="Kri1p"
/transcript_id="NM_001183146.1"
/db_xref="GeneID:855408"
CDS complement(54122..55897)
/gene="KRI1"
/locus_tag="YNL308C"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:11027267]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:22180534]"
/experiment="EXISTENCE:mutant phenotype:GO:0000447
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from
5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11027267]"
/note="Essential nucleolar protein required for 40S
ribosome biogenesis; associate with snR30; physically and
functionally interacts with Krr1p"
/codon_start=1
/product="Kri1p"
/protein_id="NP_014091.1"
/db_xref="GeneID:855408"
/db_xref="SGD:S000005252"
/translation="MPRKKSAAKRAREQAKKEAAVPATDTATIKTSETSATTVKPAIE
ASKSYVPSEDEEEDEEEEEEEDDYGELITDEVENGINQVLDAIKNNKTDKLLDPKVKF
FEDPESAAAKLANREGKHKPIYLKDYHRMNILSGDALKEDDEEYEHATVDGKQSFVSQ
QREEKTQLLNEIKSAFSDEENEESSGDEDDGFLKKKEPSTKKEGKNLPDPTVNEENFL
EEFVNQQAWIPKKGDKVISLDLNNNEEDDEEFEDAAEKFENAYNFRYEDPNAAEIISY
ARSQATLRRSDDSSRRRKREEKRKIKEQIKAEKETALQKKKTKKLNKLTDILEQLTKE
YGAEINADMVKKITDTLLKNDFKEEEWDNVVAELFNEEFYQQEGKPTWNEDDEIMGDF
YADADGDDQTEEGEVEKEQKEEDEEEGPKRKKSKKEEKLQKKKEKRKVNELVENALEQ
NKLALIEEVEKEEEERKSRSRTKEEQDLKFRYREVSPESFGLTAREIFAADDTDLNEF
IGLKKFAPYRSKELRAKDKRKVMKARRLREWRKKTFKNENGLAPVEAEAGEKDEDTIL
IPVEKASKSKHKRGHSHKHKGHQKK"
gene complement(<56446..>57573)
/gene="MCK1"
/locus_tag="YNL307C"
/gene_synonym="CMS1; YPK1"
/db_xref="GeneID:855409"
mRNA complement(<56446..>57573)
/gene="MCK1"
/locus_tag="YNL307C"
/gene_synonym="CMS1; YPK1"
/product="serine/threonine/tyrosine protein kinase MCK1"
/transcript_id="NM_001183145.1"
/db_xref="GeneID:855409"
CDS complement(56446..57573)
/gene="MCK1"
/locus_tag="YNL307C"
/gene_synonym="CMS1; YPK1"
/EC_number="2.7.12.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0004674 protein
serine/threonine kinase activity [PMID:23314252]"
/experiment="EXISTENCE:direct assay:GO:0004712 protein
serine/threonine/tyrosine kinase activity [PMID:8407952]"
/experiment="EXISTENCE:genetic interaction:GO:0030163
protein catabolic process [PMID:23236290|PMID:17954914]"
/experiment="EXISTENCE:genetic interaction:GO:0031578
mitotic spindle orientation checkpoint signaling
[PMID:36321416]"
/experiment="EXISTENCE:mutant phenotype:GO:0000070 mitotic
sister chromatid segregation [PMID:2010084]"
/experiment="EXISTENCE:mutant phenotype:GO:0004857 enzyme
inhibitor activity [PMID:22918234]"
/experiment="EXISTENCE:mutant phenotype:GO:0006303
double-strand break repair via nonhomologous end joining
[PMID:12399380]"
/experiment="EXISTENCE:mutant phenotype:GO:0015629 actin
cytoskeleton [PMID:40332024]"
/experiment="EXISTENCE:mutant phenotype:GO:0030163 protein
catabolic process [PMID:17954914|PMID:23236290]"
/experiment="EXISTENCE:mutant phenotype:GO:0030437
ascospore formation [PMID:2010083]"
/experiment="EXISTENCE:mutant phenotype:GO:0051321 meiotic
cell cycle [PMID:2010083]"
/experiment="EXISTENCE:physical interaction:GO:0030332
cyclin binding [PMID:22918234]"
/note="Dual-specificity S/T and tyrosine protein kinase;
spindle position checkpoint component that works in
parallel with Kin4p to prevent mitotic exit network (MEN)
activation by the Cdc14 early anaphase release (FEAR)
network; roles in chromosome segregation, meiotic entry,
genome stability, phosphorylation-dependent degradation of
Rcn1p and Cdc6p, inhibition of protein kinase A,
transcriptional regulation, inhibition of RNAPIII, calcium
stress and inhibition of Clb2p-Cdc28p after nuclear
division"
/codon_start=1
/product="serine/threonine/tyrosine protein kinase MCK1"
/protein_id="NP_014092.1"
/db_xref="GeneID:855409"
/db_xref="SGD:S000005251"
/translation="MSTEEQNGVPLQRGSEFIADDVTSNKSNNTRRMLVKEYRKIGRG
AFGTVVQAYLTQDKKNWLGPFAIKKVPAHTEYKSRELQILRIADHPNIVKLQYFFTHL
SPQDNKVYQHLAMECLPETLQIEINRYVTNKLEMPLKHIRLYTYQIARGMLYLHGLGV
CHRDIKPSNVLVDPETGVLKICDFGSAKKLEHNQPSISYICSRFYRAPELIIGCTQYT
TQIDIWGLGCVMGEMLIGKAIFQGQEPLLQLREIAKLLGPPDKRFIFFSNPAYDGPLF
SKPLFSGSSQQRFEKYFGHSGPDGIDLLMKILVYEPQQRLSPRRILAHQFFNELRNDD
TFLPRGFTEPIKLPNLFDFNDFELQILGEFADKIKPTKVAE"
gene <58155..>58808
/gene="MRPS18"
/locus_tag="YNL306W"
/gene_synonym="uS11m"
/db_xref="GeneID:855410"
mRNA <58155..>58808
/gene="MRPS18"
/locus_tag="YNL306W"
/gene_synonym="uS11m"
/product="mitochondrial 37S ribosomal protein uS11m
MRPS18"
/transcript_id="NM_001183144.1"
/db_xref="GeneID:855410"
CDS 58155..58808
/gene="MRPS18"
/locus_tag="YNL306W"
/gene_synonym="uS11m"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:9151978|PMID:12392552]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:9151978|PMID:12392552]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005763
mitochondrial small ribosomal subunit
[PMID:9151978|PMID:12392552]"
/note="Mitochondrial ribosomal protein of the small
subunit; essential for viability, unlike most other
mitoribosomal proteins"
/codon_start=1
/product="mitochondrial 37S ribosomal protein uS11m
MRPS18"
/protein_id="NP_014093.1"
/db_xref="GeneID:855410"
/db_xref="SGD:S000005250"
/translation="MLLQPVWKGCRWTQFVRPIRRWNSTGTNRGVPFSFKDISNQEDI
TNISYPSSSDSVLTKSNGSSEVYKPKEEVVKYILHGKFTKNNTHLTFSSVVEDKNFHK
NKGLTYNDTMLYYLNLPQKVKISLSTGCLGFRKAARGEYEAAFQTSGRMFELIKEKNM
LNKDIEVVMDDFGKGRAAFISALVGKEGASVVKKVVKISDATKLKFGGVRSPKMRRL"
gene complement(<58898..>59791)
/gene="BXI1"
/locus_tag="YNL305C"
/gene_synonym="YBH3"
/db_xref="GeneID:855411"
mRNA complement(<58898..>59791)
/gene="BXI1"
/locus_tag="YNL305C"
/gene_synonym="YBH3"
/product="Bxi1p"
/transcript_id="NM_001183143.1"
/db_xref="GeneID:855411"
CDS complement(58898..59791)
/gene="BXI1"
/locus_tag="YNL305C"
/gene_synonym="YBH3"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole
[PMID:14562095|PMID:26928762|PMID:21673659|PMID:21673967]"
/experiment="EXISTENCE:direct assay:GO:0000423 mitophagy
[PMID:38465853]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:21673659]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:21673967]"
/experiment="EXISTENCE:mutant phenotype:GO:0006915
apoptotic process [PMID:21673967|PMID:21673659]"
/experiment="EXISTENCE:mutant phenotype:GO:0019722
calcium-mediated signaling [PMID:21673967]"
/experiment="EXISTENCE:mutant phenotype:GO:0030968
endoplasmic reticulum unfolded protein response
[PMID:21673967]"
/note="Protein involved in apoptosis; contains BCL-2
homology (BH3) domain, member of BAX inhibitor family;
reported to promote apoptosis under some conditions and to
inhibit it in others; localizes to ER and vacuole;
mediates a ubiquitin-related mitophagy pathway in both
yeast and mammalian cells through association with Hsp70;
translocates to mitochondria under apoptosis-inducing
conditions in a process involving Mir1p and Cor1p"
/codon_start=1
/product="Bxi1p"
/protein_id="NP_014094.1"
/db_xref="GeneID:855411"
/db_xref="SGD:S000005249"
/translation="MSGPPPPYEEQSSHLYGQPASSQDGNAFIPEDFKYSTVVISCEP
IIRQRFMHKVYSLLSCQLLASLSFCYWASVSTSLQNFIMSHIALFYICMVVSLVSCIW
LAVSPRPEDYEASVPEPLLTGSSEEPAQEQRRLPWYVLSSYKQKLTLLSIFTLSEAYC
LSLVTLAYDKDTVLSALLITTIVVVGVSLTALSERFENVLNSATSIYYWLNWGLWIMI
GMGLTALLFGWNTHSSKFNLLYGWLGAILFTAYLFIDTQLIFRKVYPDEEVRCAMMLY
LDIVNLFLSILRILANSNDDN"
gene <60297..>61550
/gene="YPT11"
/locus_tag="YNL304W"
/db_xref="GeneID:855412"
mRNA <60297..>61550
/gene="YPT11"
/locus_tag="YNL304W"
/product="Rab family GTPase YPT11"
/transcript_id="NM_001183142.1"
/db_xref="GeneID:855412"
CDS 60297..61550
/gene="YPT11"
/locus_tag="YNL304W"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:12391144]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:23427260]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:16980630|PMID:23427260]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:23427260|PMID:12391144]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:12391144|PMID:23427260]"
/experiment="EXISTENCE:direct assay:GO:0048309 endoplasmic
reticulum inheritance [PMID:23427260]"
/experiment="EXISTENCE:genetic interaction:GO:0000001
mitochondrion inheritance [PMID:18245340|PMID:23427260]"
/experiment="EXISTENCE:genetic interaction:GO:0048312
intracellular distribution of mitochondria
[PMID:15201867|PMID:12391144]"
/experiment="EXISTENCE:genetic interaction:GO:0048313
Golgi inheritance [PMID:18595704]"
/experiment="EXISTENCE:genetic interaction:GO:0090167
Golgi distribution to daughter cells [PMID:18595704]"
/experiment="EXISTENCE:mutant phenotype:GO:0000001
mitochondrion inheritance [PMID:18245340]"
/experiment="EXISTENCE:mutant phenotype:GO:0048309
endoplasmic reticulum inheritance
[PMID:23427260|PMID:16980630]"
/experiment="EXISTENCE:mutant phenotype:GO:0048312
intracellular distribution of mitochondria
[PMID:12391144]"
/experiment="EXISTENCE:mutant phenotype:GO:0048313 Golgi
inheritance [PMID:18595704]"
/experiment="EXISTENCE:mutant phenotype:GO:0051646
mitochondrion localization [PMID:23139336]"
/experiment="EXISTENCE:mutant phenotype:GO:0090167 Golgi
distribution to daughter cells [PMID:18595704]"
/experiment="EXISTENCE:physical interaction:GO:0031489
myosin V binding [PMID:12391144]"
/note="Rab GTPase; Myo2p-binding protein implicated in
mother-to-bud transport of cortical endoplasmic reticulum
(ER), late Golgi, and mitochondria during cell division;
function is regulated at multiple levels; abundance of
active Ypt11p forms is controlled by phosphorylation
status and degradation; normally a low-abundance protein
whose ER localization is only detected when protein is
highly over expressed"
/codon_start=1
/product="Rab family GTPase YPT11"
/protein_id="NP_014095.2"
/db_xref="GeneID:855412"
/db_xref="SGD:S000005248"
/translation="MSQRKRYSLNVVTSPSIPSPTPSAPIRTNESNWEAASPASAASS
FLPNVHHGGTVLNPGLGIMRSPSLNKSGAFGRSGSSGSSTVIEPSNIKLLLIGDANVG
KTAMILSYCRELLTRAEMSRSARLRHQQQQQHKDLGLKKTVVNHRLSMKEKRKRYSSN
DFEKEFKDINHFADETSDFGNPNIGDDNNHEMADPNEIVIETRSTIGIDIKTNLVNID
NRFFNVILWDTAGQERYQNAIIPSLYKKTNAVILTYDITNAKSFQSCMERWIVQALEN
FSSQDLLKARFFLVGNKIDLYKERQVTHYDVVQMVQEMQLKHGIKISGNFEVSCKWVN
VVERTMNMIILDLVENGCFENNDPCVSITTSDDVQGHEQEFHDTVEEPFNFTRQRQHQ
LEKNNTVDITKPNDDIANNQSICCV"
rep_origin 61599..61895
/note="ARS1406; Autonomously Replicating Sequence"
/db_xref="SGD:S000118383"
gene complement(<61958..>62943)
/gene="RPS19B"
/locus_tag="YNL302C"
/gene_synonym="RP55B"
/db_xref="GeneID:855414"
mRNA complement(join(<61958..62372,62924..>62943))
/gene="RPS19B"
/locus_tag="YNL302C"
/gene_synonym="RP55B"
/product="40S ribosomal protein eS19 RPS19B"
/transcript_id="NM_001183140.1"
/db_xref="GeneID:855414"
CDS complement(join(61958..62372,62924..62943))
/gene="RPS19B"
/locus_tag="YNL302C"
/gene_synonym="RP55B"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:6814480]"
/experiment="EXISTENCE:direct assay:GO:0022627 cytosolic
small ribosomal subunit [PMID:6814480]"
/experiment="EXISTENCE:genetic interaction:GO:0000054
ribosomal subunit export from nucleus
[PMID:16159874|PMID:16246728]"
/experiment="EXISTENCE:genetic interaction:GO:0042274
ribosomal small subunit biogenesis [PMID:16159874]"
/experiment="EXISTENCE:mutant phenotype:GO:0000054
ribosomal subunit export from nucleus [PMID:16159874]"
/experiment="EXISTENCE:mutant phenotype:GO:0042274
ribosomal small subunit biogenesis [PMID:16159874]"
/note="Protein component of the small (40S) ribosomal
subunit; required for assembly and maturation of pre-40 S
particles; homologous to mammalian ribosomal protein S19,
no bacterial homolog; mutations in human RPS19 are
associated with Diamond Blackfan anemia; RPS19B has a
paralog, RPS19A, that arose from the whole genome
duplication"
/codon_start=1
/product="40S ribosomal protein eS19 RPS19B"
/protein_id="NP_014097.1"
/db_xref="GeneID:855414"
/db_xref="SGD:S000005246"
/translation="MAGVSVRDVAAQDFINAYASFLQRQGKLEVPGYVDIVKTSSGNE
MPPQDAEGWFYKRAASVARHIYMRKQVGVGKLNKLYGGAKSRGVRPYKHIDASGSINR
KVLQALEKIGIVEISPKGGRRISENGQRDLDRIAAQTLEEDE"
gene complement(<63570..>64562)
/gene="RPL18B"
/locus_tag="YNL301C"
/gene_synonym="RP28B"
/db_xref="GeneID:855415"
mRNA complement(join(<63570..64018,64451..>64562))
/gene="RPL18B"
/locus_tag="YNL301C"
/gene_synonym="RP28B"
/product="60S ribosomal protein eL18 RPL18B"
/transcript_id="NM_001183139.1"
/db_xref="GeneID:855415"
CDS complement(join(63570..64018,64451..64562))
/gene="RPL18B"
/locus_tag="YNL301C"
/gene_synonym="RP28B"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:11983894]"
/experiment="EXISTENCE:curator inference:GO:0003735
structural constituent of ribosome [PMID:11983894]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:11983894]"
/note="Ribosomal 60S subunit protein L18B; homologous to
mammalian ribosomal protein L18, no bacterial homolog;
RPL18B has a paralog, RPL18A, that arose from the whole
genome duplication"
/codon_start=1
/product="60S ribosomal protein eL18 RPL18B"
/protein_id="NP_014098.1"
/db_xref="GeneID:855415"
/db_xref="SGD:S000005245"
/translation="MGIDHTSKQHKRSGHRTAPKSDNVYLKLLVKLYTFLARRTDAPF
NKVVLKALFLSKINRPPVSVSRIARALKQEGAANKTVVVVGTVTDDARIFEFPKTTVA
ALRFTAGARAKIVKAGGECITLDQLAVRAPKGQNTLILRGPRNSREAVRHFGMGPHKG
KAPRILSTGRKFERARGRRRSKGFKV"
gene <65744..>66052
/gene="TOS6"
/locus_tag="YNL300W"
/db_xref="GeneID:855416"
mRNA <65744..>66052
/gene="TOS6"
/locus_tag="YNL300W"
/product="Tos6p"
/transcript_id="NM_001183138.1"
/db_xref="GeneID:855416"
CDS 65744..66052
/gene="TOS6"
/locus_tag="YNL300W"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:10383953]"
/note="Glycosylphosphatidylinositol-dependent cell wall
protein; expression is periodic and decreases in respone
to ergosterol perturbation or upon entry into stationary
phase; depletion increases resistance to lactic acid"
/codon_start=1
/product="Tos6p"
/protein_id="NP_014099.1"
/db_xref="GeneID:855416"
/db_xref="SGD:S000005244"
/translation="MKFSTLSTVAAIAAFASADSTSDGVTYVDVTTTPQSTTSMVSTV
KTTSTPYTTSTIATLSTKSISSQANTTTHEISTYVGAAVKGSVAGMGAIMGAAAFALL
"
gene <66517..>68445
/gene="TRF5"
/locus_tag="YNL299W"
/db_xref="GeneID:855417"
mRNA <66517..>68445
/gene="TRF5"
/locus_tag="YNL299W"
/product="non-canonical poly(A) polymerase TRF5"
/transcript_id="NM_001183137.1"
/db_xref="GeneID:855417"
CDS 66517..68445
/gene="TRF5"
/locus_tag="YNL299W"
/EC_number="2.7.7.19"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0031499 TRAMP
complex [PMID:16374505]"
/experiment="EXISTENCE:direct assay:GO:1990817 poly(A) RNA
polymerase activity [PMID:16260630|PMID:16374505]"
/experiment="EXISTENCE:genetic interaction:GO:0034475 U4
snRNA 3'-end processing [PMID:16373491]"
/experiment="EXISTENCE:genetic interaction:GO:0071035
nuclear polyadenylation-dependent rRNA catabolic process
[PMID:15935758|PMID:17652137]"
/experiment="EXISTENCE:genetic interaction:GO:0071036
nuclear polyadenylation-dependent snoRNA catabolic process
[PMID:15935758|PMID:16373491]"
/experiment="EXISTENCE:genetic interaction:GO:0071037
nuclear polyadenylation-dependent snRNA catabolic process
[PMID:15935758]"
/experiment="EXISTENCE:genetic interaction:GO:0071038
TRAMP-dependent tRNA surveillance pathway [PMID:16431988]"
/experiment="EXISTENCE:genetic interaction:GO:0071039
nuclear polyadenylation-dependent CUT catabolic process
[PMID:18007593]"
/experiment="EXISTENCE:genetic interaction:GO:0071042
nuclear polyadenylation-dependent mRNA catabolic process
[PMID:16373491]"
/experiment="EXISTENCE:genetic interaction:GO:0071044
histone mRNA catabolic process [PMID:17179095]"
/experiment="EXISTENCE:genetic interaction:GO:0071051
poly(A)-dependent snoRNA 3'-end processing
[PMID:16373491]"
/experiment="EXISTENCE:genetic interaction:GO:1990817
poly(A) RNA polymerase activity [PMID:16374505]"
/experiment="EXISTENCE:mutant phenotype:GO:0034475 U4
snRNA 3'-end processing [PMID:16373491]"
/experiment="EXISTENCE:mutant phenotype:GO:0071035 nuclear
polyadenylation-dependent rRNA catabolic process
[PMID:15935758|PMID:18007593]"
/experiment="EXISTENCE:mutant phenotype:GO:1990817 poly(A)
RNA polymerase activity [PMID:16374505]"
/note="Non-canonical poly(A) polymerase; involved in
nuclear RNA degradation as a component of the TRAMP
complex; catalyzes polyadenylation of hypomodified tRNAs,
and snoRNA and rRNA precursors; overlapping but
non-redundant functions with Pap2p"
/codon_start=1
/product="non-canonical poly(A) polymerase TRF5"
/protein_id="NP_014100.2"
/db_xref="GeneID:855417"
/db_xref="SGD:S000005243"
/translation="MTRLKAKYSPTKGKRKEDKHTKRMRKSSFTRTQKMLEVFNDNRS
HFNKYESLAIDVEDDDTFGNLVLMENDKSDVDIPVIEEVTSSEDEQRAESSKRNNSLE
DNQDFIAFSDSSEDETEQIKEDDDERSSFLLTDEHEVSKLTSQQSLNTESACNVEYPW
IRNHCHSKQRRIADWLTSEIKDFVHYISPSKNEIKCRNRTIDKLRRAVKELWSDADLH
VFGSFATDLYLPGSDIDCVVNSRNRDKEDRNYIYELARHLKNKGLAIRMEVIVKTRVP
IIKFIEPQSQLHIDVSFERTNGLEAAKLIREWLRDSPGLRELVLIIKQFLHSRRLNNV
HTGGLGGFTVICLVYSFLNMHPRIKSNDIDVLDNLGVLLIDFFELYGKNFGYDDVAIS
ISDGYASYIPKSCWRTLEPSRSKFSLAIQDPGDPNNNISRGSFNMKDIKKAFAGAFEL
LVNKCWELNSATFKDRVGKSILGNVIKYRGQKRDFNDERDLVQNKAIIENERYHKRRT
RIVQEDLFINDTEDLPVEEIYKLDEPAKKKQKAKKDKREGEIKKSAIPSPPPDFGVSR
SKLKRKVKKTDQGSLLHQNNLSIDDLMGLSENDQESDQDQKGRDTPSGQDEKSPLETK
TVDAQTRRDYWLSKGQAL"
gene <68915..>71443
/gene="CLA4"
/locus_tag="YNL298W"
/gene_synonym="ERC10"
/db_xref="GeneID:855418"
mRNA <68915..>71443
/gene="CLA4"
/locus_tag="YNL298W"
/gene_synonym="ERC10"
/product="serine/threonine protein kinase CLA4"
/transcript_id="NM_001183136.1"
/db_xref="GeneID:855418"
CDS 68915..71443
/gene="CLA4"
/locus_tag="YNL298W"
/gene_synonym="ERC10"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0000278 mitotic
cell cycle [PMID:22588722]"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:19218422]"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0004674 protein
serine/threonine kinase activity [PMID:22588722]"
/experiment="EXISTENCE:direct assay:GO:0005933 cellular
bud [PMID:19218422]"
/experiment="EXISTENCE:direct assay:GO:0016301 kinase
activity [PMID:14993234]"
/experiment="EXISTENCE:direct assay:GO:0045033 peroxisome
inheritance [PMID:39318281]"
/experiment="EXISTENCE:genetic interaction:GO:0000011
vacuole inheritance [PMID:19218422]"
/experiment="EXISTENCE:genetic interaction:GO:0010629
negative regulation of gene expression [PMID:19793923]"
/experiment="EXISTENCE:genetic interaction:GO:0019236
response to pheromone [PMID:17189484]"
/experiment="EXISTENCE:genetic interaction:GO:0031106
septin ring organization [PMID:7649470]"
/experiment="EXISTENCE:mutant phenotype:GO:0000011 vacuole
inheritance [PMID:19218422]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:19793923]"
/experiment="EXISTENCE:mutant phenotype:GO:0005634 nucleus
[PMID:19793923]"
/experiment="EXISTENCE:mutant phenotype:GO:0007096
regulation of exit from mitosis [PMID:12234925]"
/experiment="EXISTENCE:mutant phenotype:GO:0010629
negative regulation of gene expression [PMID:19793923]"
/experiment="EXISTENCE:mutant phenotype:GO:0031106 septin
ring organization [PMID:11025657]"
/experiment="EXISTENCE:mutant phenotype:GO:0035376 sterol
import [PMID:19793923]"
/experiment="EXISTENCE:mutant phenotype:GO:0042307
positive regulation of protein import into nucleus
[PMID:22588722]"
/experiment="EXISTENCE:mutant phenotype:GO:2000910
negative regulation of sterol import [PMID:19793923]"
/note="Cdc42p-activated signal transducing kinase; member
of the PAK (p21-activated kinase) family, along with
Ste20p and Skm1p; involved in septin ring assembly,
vacuole inheritance, cytokinesis, sterol uptake
regulation; phosphorylates Cdc3p and Cdc10p; CLA4 has a
paralog, SKM1, that arose from the whole genome
duplication"
/codon_start=1
/product="serine/threonine protein kinase CLA4"
/protein_id="NP_014101.1"
/db_xref="GeneID:855418"
/db_xref="SGD:S000005242"
/translation="MSLSAAANKISDNDFQNIGPAPRPPSSNSQGRTCYNQTQPITKL
MSQLDLTSASHLGTSTSKKKSGWVSYKDDGILSFIWQKRYLMLHDSYVALYKNDKQND
DAILKIPLTSIISVSRTQLKQYCFELVRCSDRNSVSSGSSSSLNVSSDSNSKKSIYIA
TKTESDLHSWLDAIFAKCPLLSGVSSPTNFTHKVHVGFDPETGSFVGMPTNWEKLLKH
SRITGEDWNNNSAAVIQVLQFYQEYNGAGNPTNTLDKPQSGETSSSQKSLPNSYNDNK
LRNNSVNSKSSSGVSSSMVSQRKTSQPPNTKSPVSLGSGSLPPINTKLPTSQSNIPRH
LQNVPNQQYPKMRNGHSPTNGQFPRGPMHPNNSQRSLQQQQQQQQQQKQQHQQYPYHH
QGPSPSPSPSPSPLNPYRPHHNMINPYSKQPQSPLSSQSTQNQAIPRYAQNSSPTAAH
FQPQRTAPKPPISAPRAPYPSNQNATSNTHVQPVAPKNDQSTPQTMRQAPKRPDADVA
QPGGVAKPKKPARPTMSTAEIMSKLKKVTVNADPSQCFKVIEKAGQGASGSVYLAERT
HIPTESNMIELINNDIDEPHVGDKVAIKQMVLSKQPRKELIVNEILVMKDSRHKNIVN
FLEAYLRTDDDLWVVMEFMEGGSLTDIIENSPTNDNSHSPLTEPQIAYIVRETCQGLK
FLHDKHIIHRDIKSDNVLLDTRARVKITDFGFCARLTDKRSKRATMVGTPYWMAPEVV
KQREYDEKIDVWSLGIMTIEMLEGEPPYLNEDPLKALYLIATNGTPKLKHPESLSLEI
KRFLSVCLCVDVRYRASTEELLHHGFFNMACDPKDLTSLLEWKE"
gene complement(<71673..>76583)
/gene="MON2"
/locus_tag="YNL297C"
/gene_synonym="YSL2"
/db_xref="GeneID:855420"
mRNA complement(<71673..>76583)
/gene="MON2"
/locus_tag="YNL297C"
/gene_synonym="YSL2"
/product="Mon2p"
/transcript_id="NM_001183135.1"
/db_xref="GeneID:855420"
CDS complement(71673..76583)
/gene="MON2"
/locus_tag="YNL297C"
/gene_synonym="YSL2"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:12052896]"
/experiment="EXISTENCE:direct assay:GO:0005802 trans-Golgi
network [PMID:16301316]"
/experiment="EXISTENCE:direct assay:GO:0006623 protein
targeting to vacuole [PMID:16219684]"
/experiment="EXISTENCE:direct assay:GO:0031901 early
endosome membrane [PMID:16219684]"
/experiment="EXISTENCE:genetic interaction:GO:0005085
guanyl-nucleotide exchange factor activity
[PMID:12052896]"
/experiment="EXISTENCE:mutant phenotype:GO:0006623 protein
targeting to vacuole [PMID:12134085]"
/experiment="EXISTENCE:mutant phenotype:GO:0006897
endocytosis [PMID:12052896]"
/experiment="EXISTENCE:mutant phenotype:GO:0042147
retrograde transport, endosome to Golgi [PMID:16301316]"
/experiment="EXISTENCE:physical interaction:GO:0005085
guanyl-nucleotide exchange factor activity
[PMID:12052896]"
/note="Protein with a role in endocytosis and vacuole
integrity; peripheral membrane protein; interacts with and
negatively regulates Arl1p; localizes to the endosome;
member of the Sec7p family of proteins"
/codon_start=1
/product="Mon2p"
/protein_id="NP_014102.1"
/db_xref="GeneID:855420"
/db_xref="SGD:S000005241"
/translation="MAMNTGGFDSMQRQLEAELRSLSSESKRRNSTIRHASDKSIEIL
KRVHSFEELERHPDFALPFVLACQSRNAKMTTLAMQCLQGLSTVPSIPRSRLSEILDA
FIEATHLAMEIQLKVLQVVPIFFKTYGKFIYGPLCKKLLLCCSNLLHVPNKAPVVVGT
ASATLQQLIDEIFDRLSIESVVDDKQYEVLISNSESIKVNVYRYDANKLFDNICSLNE
ISSNGAVSDEEMLLDIGDIPIDYGLEILESILKNSQKNLLECQDLQYLLRVKAIPLLL
RCISSSRHFSTAVRSCRCLKLLIRKEYLSILELELEVILSLLIHGISVESNLSGWQRV
LSLELFKDLSQDPEIVNTLYMDYDNYPDKKHVFKYLLKECIVLLNSPEYITFLAPSKV
VEKMDSPLITTENSTVKTKFMHLLDKSNAPSINITYIISLILTICNHLCEGLNKSALE
SSPLEKKIEDKEREEGTGNDSTVVKVYSGLFSGLFELNKLFLYSTSLETSIFHLVVRA
FQKLAHSAGVLSLKDKLRACMKLFSILITNNVTSSNQYSFNDTSKSAKNQHTRNISTS
SVTTSPVESTKNPSRSIADSAQNKEMKRRLHPRNISSRQVSLLRALISLSISLGPIFD
SESWRYTFLTWQWITYYIYGPSADFKESFYSEDIPPPPILTKSDVTSIESSLTKFFES
TSSYSCSTFHLVLTRLILDSKNTLTLEQTNLNLNNDIGYHPLDAKDEIIPCIYNKAFF
VNKIGELATYNCKKFLFGKNGKELWSLISTYMIKLISNREMDNDSLRLYTVRVFTDII
KKATNEVGNSDEQDNKVKQFGTLENLVIDSLMATINSIKQLDIGKQEIYNGTINVESD
ILFQLLLTLKEILNEFGELLMNSWTNIFNIINSPFEWTVEDTDFSVNEDIDDSSLFEG
IVQKHKNMIQVSYDVFKLISDDFLQSLPMSVIKFVIDTLVNFVSQKRNLNISFSSISQ
FWLVGDYLRVRFNPETLNLSDEKRRSLSEKINNQKLIEIITSSSSHDWELYNGLWIYL
LKNLINCTNDDRVEVKNGAVQTFFRIIDSHSVCFPPWDLIFLEVIEPLLTKEWSTEEL
ENETDFINVTLQGLIKLYPEHFKDFKNNTTCAKEWSMLLDFLKRLLSSTSNNTKNAVI
LNYQTLLKEIITIEDVPSDILKKCCEIFTDYNITYSDLSTNASSKTEYDCIYELITGF
PPLYQLISKYDAMTDEFVEKVLLLFNSAIKYPLLPEFVQDKTKPSSMQKAILSGLDIF
MTNDSKDTEILILLQLSTISILAFDTREKITKKLGPKLPKASLNRLPTFEAISYMSCS
NLRNRIAKIDQFGISTLKAKHILRILKNLAEIIKRKSLITGSESDEIPIWVLASNCFC
DLSNKIFKSLQEDAENPLKDNFCDLFINVIVVTLQRINPELDNLTEIDDLNEYSKYRE
ILLENRIIDLFNERQLDTFIYAVWDCSFLYEFDELENALMQDCGTFSELSQKLSSFDF
SCIFGSTTNPRFQTKYKCSLECLQDLVNFMLNTNEKLRKLTAPYLSARIALALRRYIS
DEYLIGRAPIPKLRKTELATLLNGLCVILRGVLDQNSTLGNKQIGVENLQTLSPLILR
TIPVSHKMDGLQDKVLELSLGFTKLD"
gene <76946..>78520
/gene="MRX6"
/locus_tag="YNL295W"
/db_xref="GeneID:855421"
mRNA <76946..>78520
/gene="MRX6"
/locus_tag="YNL295W"
/product="Mrx6p"
/transcript_id="NM_001183133.1"
/db_xref="GeneID:855421"
CDS 76946..78520
/gene="MRX6"
/locus_tag="YNL295W"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:31532710]"
/experiment="EXISTENCE:mutant phenotype:GO:1901858
regulation of mitochondrial DNA metabolic process
[PMID:31532710]"
/note="Mitochondrial ribosome-associated protein;
regulates mitochondrial DNA copy number and the size and
distribution of mtDNA nucleoids; subunit of a complex
containing Pet20p, Mam33p, and Pim1p that may regulate
mtDNA replication; member of the PET20 domain-containing
protein family; forms foci that partially co-localize with
mtDNA"
/codon_start=1
/product="Mrx6p"
/protein_id="NP_014104.1"
/db_xref="GeneID:855421"
/db_xref="SGD:S000005239"
/translation="MEHQALRRLVLYCPNFIRRGALRQNMTRVSCRHMSGKGGGRDEK
GDCNEEKDSSKDLGRVPSKMKRAYDGETVIKEGDSHAESLAQQGKQPTDLAYNSRSKI
SGSNLHLLVPRVASTDYISNKEVHTEGLFAGYRPLFLGNSGFPSDARKGKNFHELDDV
LPNIQVVDASEKDGKLNVQEIIEDLQRTSLRESIHSMEQLPSSHKRKPVIPWDASISG
MVYNDMPFKYVPKNIILKMKPFKLLRIERKSQAKNARKPTMIKLQFHNRRINDTPELV
NLYHNKSRLHESLYNTKPLQESGYSSANTSKRQKMLKARSDFEHKQKNYAYKHTFIKN
DQELFRNELTKLNKILAREFKKLTKLSIHNEFKREHLPLAVYVSKSKGTKKLFRRSLK
MKIMDHIYPVYTTILSTLTNSRDSKKFENKIKAYIEKIIVRLSDEVPSTYFFQDGVDC
IIQPSPIHNFKRMHWLRYTKRHNTFWGRTIHKDVQVSFNDKYVVTRSGVRYTRYPTNL
NTQLLETAFEEWDYYE"
gene complement(<78659..>80260)
/gene="RIM21"
/locus_tag="YNL294C"
/gene_synonym="PAL2"
/db_xref="GeneID:855422"
mRNA complement(<78659..>80260)
/gene="RIM21"
/locus_tag="YNL294C"
/gene_synonym="PAL2"
/product="Rim21p"
/transcript_id="NM_001183132.1"
/db_xref="GeneID:855422"
CDS complement(78659..80260)
/gene="RIM21"
/locus_tag="YNL294C"
/gene_synonym="PAL2"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:23019326]"
/experiment="EXISTENCE:genetic interaction:GO:0009272
fungal-type cell wall biogenesis [PMID:16524906]"
/experiment="EXISTENCE:genetic interaction:GO:0044088
regulation of vacuole organization [PMID:25359886]"
/experiment="EXISTENCE:mutant phenotype:GO:0001403
invasive growth in response to glucose limitation
[PMID:10821185]"
/experiment="EXISTENCE:mutant phenotype:GO:0009272
fungal-type cell wall biogenesis [PMID:16524906]"
/experiment="EXISTENCE:mutant phenotype:GO:0030437
ascospore formation [PMID:10821185]"
/experiment="EXISTENCE:mutant phenotype:GO:0071469
cellular response to alkaline pH [PMID:23019326]"
/note="pH sensor molecule, component of RIM101 pathway;
positive regulator of cell size; has role in cell wall
construction and alkaline pH response; is glycosylated and
phosphorylated; interacts with Dfg16p and Rim9p to form a
pH-sensing complex; localization to plasma membrane is
dependent on Dfg16p and Rim9p; has similarity to A.
nidulans PalH"
/codon_start=1
/product="Rim21p"
/protein_id="NP_014105.1"
/db_xref="GeneID:855422"
/db_xref="SGD:S000005238"
/translation="MNLWRHSPEELAAYNSCHPMKLGSGVLIQLPLYDNSAVYAEDIT
FRSFCCERVPVYVSTVLRNSSPYRYLDEVINDWQKFIQVSDYVGGSAEYAIYAVILSI
TSNFVITVFLTVICCINISGRAYKRILQLLRIASLLASLNLTIFITKVLRRLEKEHNV
YGVVRAHSIMHIFSDDMTFVVLDFLATLMFQFCQVGIVIRLFQRAQEKRIIFFIGVIL
TITANILWVIPPFANHTTKHRNDWQILRPFVYLFRIAIATSYASIVIYHIWQKKKLWF
KFNQMGLLTLLTILVVLLLPGFFLADVSNLWISELGEVFNTTCYVTSTVITWEWLDRL
NVLERKEEAQSILGRPIFEEEQQDYRFAKYALRVQNALTRRESQDASTDRHDTSSNSE
VCDLQTISRYDPEDQISVGRSIDRMHFNDRGTYKDVALKKLGYARDKILYFTDQIVQK
SVGHNNSSSSKNEKTKQRKAMVRKRLGLDKPGIYIYSTKDVVFNSDEDDDENAEDEDD
DEYEVGSEGNNNSSATFTSDHIGHI"
gene <80640..>82541
/gene="MSB3"
/locus_tag="YNL293W"
/gene_synonym="GYP3"
/db_xref="GeneID:855423"
mRNA <80640..>82541
/gene="MSB3"
/locus_tag="YNL293W"
/gene_synonym="GYP3"
/product="Rab GTPase-activating protein MSB3"
/transcript_id="NM_001183131.1"
/db_xref="GeneID:855423"
CDS 80640..82541
/gene="MSB3"
/locus_tag="YNL293W"
/gene_synonym="GYP3"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:10679030]"
/experiment="EXISTENCE:direct assay:GO:0000133 polarisome
[PMID:16166638]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:22593206]"
/experiment="EXISTENCE:direct assay:GO:0005096 GTPase
activator activity [PMID:22593206|PMID:10559187]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005770 late
endosome [PMID:22593206]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22593206]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:22593206]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:22842922|PMID:10679030]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:22842922|PMID:22593206|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:35134079]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0006887
exocytosis [PMID:12913108]"
/experiment="EXISTENCE:genetic interaction:GO:0070649
formin-nucleated actin cable assembly [PMID:16166638]"
/experiment="EXISTENCE:mutant phenotype:GO:0005096 GTPase
activator activity [PMID:22593206]"
/experiment="EXISTENCE:mutant phenotype:GO:0006897
endocytosis [PMID:22593206]"
/experiment="EXISTENCE:mutant phenotype:GO:0032880
regulation of protein localization [PMID:22593206]"
/experiment="EXISTENCE:physical interaction:GO:0006897
endocytosis [PMID:22593206]"
/experiment="EXISTENCE:physical interaction:GO:0032880
regulation of protein localization [PMID:22593206]"
/experiment="EXISTENCE:physical interaction:GO:0070649
formin-nucleated actin cable assembly [PMID:16166638]"
/note="Rab GTPase-activating protein; regulates
endocytosis via inactivation of Vps21p at endosomes and
vacuole fusion via inactivation of Ypt7p at vacuoles; also
acts on Ypt52p and Sec4p; localizes to plasma membrane,
sites of polarized growth; relocalizes from bud neck to
cytoplasm upon DNA replication stress; similar to
TBC-domain Tre2 oncogene; MSB3 has a paralog, MSB4, that
arose from the whole genome duplication; human homolog
USP6NL can complement yeast msb3 msb4 double null"
/codon_start=1
/product="Rab GTPase-activating protein MSB3"
/protein_id="NP_014106.1"
/db_xref="GeneID:855423"
/db_xref="SGD:S000005237"
/translation="MQNDQQRFSLQNRTVLAHPYKRLGGAFTVKSPSVPNFHDKMHSD
HSSSDSALVNGSFRANDHRSVEPSCLGQASPSEHDGNLSVIDLYGDEVESQRAEGEDD
DDNNGDNGNEDLEEVHSDDLDLVPDDDNRQRVELEGAASATSANSNGINNTHFDRYGF
KKQNNYISEAEYDKWWVEYSQYCVRRKHKWQLLLEKSGLPVTDDSPSRFPSKSERLKR
YVRKGIPAEWRGNAWWHFARGQEKLNKNKGVYSQLLRKMKQIKKQNPNEKQVQDLDII
ERDLNRTFPDNIHFQSSLHNKEGPPIIKSLRRVLVAFSLYNPKIGYCQSMNFLAGLLL
LFLDEERAFWMLVIITSRYLPGVHNINLEGVNIDQGVLMLCVKEYIPEVWSYIKPSID
HHQKNNKTFSPSNKKVLFNMQKNEFLYRLPPITLCTASWFMSCFVGVVPIETTLRIWD
CLFYEESHFLFKVSLAVLKLSEHDLSKIKPRNNSLNYSWGSNLNQRGGSMGQEDSDME
IFQVIQTFPKTLLNPNEIFEKIIFKRRFNLNRLDQDEIDRCRKFVAAQRLKFKTYGEL
LGNSTSEADLPINDNTDNKGIHITSDAVNEALSSEVYGFKKSLAGVHWNNSIKEKVKQ
MRKKKDKGD"
gene <82806..>84017
/gene="PUS4"
/locus_tag="YNL292W"
/db_xref="GeneID:855424"
mRNA <82806..>84017
/gene="PUS4"
/locus_tag="YNL292W"
/product="pseudouridine synthase PUS4"
/transcript_id="NM_001183130.1"
/db_xref="GeneID:855424"
CDS 82806..84017
/gene="PUS4"
/locus_tag="YNL292W"
/EC_number="5.4.99.25"
/experiment="EXISTENCE:direct assay:GO:0006400 tRNA
modification [PMID:9358157]"
/experiment="EXISTENCE:direct assay:GO:0009982
pseudouridine synthase activity [PMID:9358157]"
/experiment="EXISTENCE:genetic interaction:GO:0009982
pseudouridine synthase activity [PMID:9358157]"
/experiment="EXISTENCE:mutant phenotype:GO:0005634 nucleus
[PMID:9358157]"
/experiment="EXISTENCE:mutant phenotype:GO:0005739
mitochondrion [PMID:9358157]"
/experiment="EXISTENCE:mutant phenotype:GO:1990481 mRNA
pseudouridine synthesis [PMID:25192136|PMID:25219674]"
/note="Pseudouridine synthase; catalyzes only the
formation of pseudouridine-55 (Psi55), a highly conserved
tRNA modification, in mitochondrial and cytoplasmic tRNAs;
also responsible for pseudouracil modification of some
mRNAs; PUS4 overexpression leads to translational
derepression of GCN4 (Gcd- phenotype)"
/codon_start=1
/product="pseudouridine synthase PUS4"
/protein_id="NP_014107.1"
/db_xref="GeneID:855424"
/db_xref="SGD:S000005236"
/translation="MNGIFAIEKPSGITSNQFMLKLQHALTKSQVFSKEIQRATAERK
QQYEKQTGKKASKRKLRKVSKVKMGHGGTLDPLASGVLVIGIGAGTKKLANYLSGTVK
VYESEALFGVSTTSGDVEGEILSQNSVKHLNFDDLKTVEEKFVGQLKQTPPIYAALKM
DGKPLHEYAREGKPLPRAIEPRQVTIYDLKVFSDSLKRDHDYPLLRPTTEEAVDTVKN
LNANMLNDVLYFSKEYTEKHGLDSEVAKVEEPFPLSEQEEQEIQKEGDSYRAPKLHFK
ANVSSGTYIRSLVSDIGKSMRSSCYMVKLIRLQQQDWSLEKNNVFQLTDFTERDEKVW
SKVLEKVLDEGATVDVIEELKKAEKEIPADVKECIVSSDQPGDEATAETIETANAEEH
SNTLKRKIEQV"
gene complement(<84169..>85815)
/gene="MID1"
/locus_tag="YNL291C"
/db_xref="GeneID:855425"
mRNA complement(<84169..>85815)
/gene="MID1"
/locus_tag="YNL291C"
/product="Mid1p"
/transcript_id="NM_001183129.1"
/db_xref="GeneID:855425"
CDS complement(84169..85815)
/gene="MID1"
/locus_tag="YNL291C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14729456]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:10958666]"
/experiment="EXISTENCE:direct assay:GO:0006816 calcium ion
transport [PMID:10958666]"
/experiment="EXISTENCE:direct assay:GO:0015275
stretch-activated, monoatomic cation-selective, calcium
channel activity [PMID:10436155]"
/experiment="EXISTENCE:mutant phenotype:GO:0005262 calcium
channel activity [PMID:12492866|PMID:9343395]"
/note="Stretch-activated Ca2+-permeable cation channel;
required for Ca2+ influx stimulated by mating pheromones
and some abiotic stresses; sensor of compressive
mechanical stress; N-glycosylated membrane protein of the
ER and plasma membrane; interacts with Cch1p;
oligomerizes; is an alpha2/delta-like protein and
participates in the generation of stretch-activated
Ca2+-permeable cation channel activity when expressed in
mammalian cells"
/codon_start=1
/product="Mid1p"
/protein_id="NP_014108.1"
/db_xref="GeneID:855425"
/db_xref="SGD:S000005235"
/translation="MIVWQALFVVYCLFTTSIHGLFQDFNPFANKNISLKFPSLNRWE
KNVMATGQQTIINSDSIYEWTPILSNITAGKKDSFVFTIDAEASGYGFAPTYEVLMFI
SGNICQMPMNRSDVDLTIYYSFNETVLENPNIGQSAVFQDGYIQALAISPVQSSSSNA
TSTYSNLYVVAELVNSTTEQPLSSSDASENWEYRLSISENDLVFQWDVRPWVEVLDTD
MNSALLSTGNVTADAKVYHNYSIYDPSLYDLYVYSYEDSVQLNQNYNLSLCAVKNGPY
LVSSQNTSNATVTSNSTNPLERTDLAIQKKITEYGGSVTEMFYVTGLNASTTYVAYLT
KKISNGDGLSSVGGILFSHVYFTTRSTDVCSLIFGLDFCSDVAYSVPTSSFSVGNKTL
MAQTYDHIAEALYANFSKALQLISCDADKDARYSPVMTCDDCAEAYRDWVCAVSIPRC
TTTSSQYYIHRDKSHNRNDYLNKFIKPLDDYYEILPCIDMCYTLVRNCPSDFQFSCPN
DLTTEDLLYQSYNFYMDTDYSTCNYIGNSSLMVIHPLDDT"
gene <86218..>87240
/gene="RFC3"
/locus_tag="YNL290W"
/db_xref="GeneID:855426"
mRNA <86218..>87240
/gene="RFC3"
/locus_tag="YNL290W"
/product="replication factor C subunit 3"
/transcript_id="NM_001183128.1"
/db_xref="GeneID:855426"
CDS 86218..87240
/gene="RFC3"
/locus_tag="YNL290W"
/experiment="EXISTENCE:direct assay:GO:0003689 DNA clamp
loader activity [PMID:12604797|PMID:8995429]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476|PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005663 DNA
replication factor C complex [PMID:7651383]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0006272 leading
strand elongation [PMID:1346062]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:8302859]"
/experiment="EXISTENCE:direct assay:GO:0031389 Rad17
RFC-like complex [PMID:12604797|PMID:10660302]"
/experiment="EXISTENCE:physical interaction:GO:0031389
Rad17 RFC-like complex [PMID:10660302]"
/experiment="EXISTENCE:physical interaction:GO:0031390
Ctf18 RFC-like complex [PMID:11389843]"
/experiment="EXISTENCE:physical interaction:GO:0031391
Elg1 RFC-like complex [PMID:12912927]"
/note="Subunit of heteropentameric Replication factor C
(RF-C); which is a DNA binding protein and ATPase that
acts as a clamp loader of the proliferating cell nuclear
antigen (PCNA) processivity factor for DNA polymerases
delta and epsilon; relocalizes to the cytosol in response
to hypoxia"
/codon_start=1
/product="replication factor C subunit 3"
/protein_id="NP_014109.1"
/db_xref="GeneID:855426"
/db_xref="SGD:S000005234"
/translation="MSTSTEKRSKENLPWVEKYRPETLDEVYGQNEVITTVRKFVDEG
KLPHLLFYGPPGTGKTSTIVALAREIYGKNYSNMVLELNASDDRGIDVVRNQIKDFAS
TRQIFSKGFKLIILDEADAMTNAAQNALRRVIERYTKNTRFCVLANYAHKLTPALLSR
CTRFRFQPLPQEAIERRIANVLVHEKLKLSPNAEKALIELSNGDMRRVLNVLQSCKAT
LDNPDEDEISDDVIYECCGAPRPSDLKAVLKSILEDDWGTAHYTLNKVRSAKGLALID
LIEGIVKILEDYELQNEETRVHLLTKLADIEYSISKGGNDQIQGSAVIGAIKASFENE
TVKANV"
gene <87897..>88736
/gene="PCL1"
/locus_tag="YNL289W"
/gene_synonym="HCS26"
/db_xref="GeneID:855427"
mRNA <87897..>88736
/gene="PCL1"
/locus_tag="YNL289W"
/gene_synonym="HCS26"
/product="Pcl1p"
/transcript_id="NM_001183127.1"
/db_xref="GeneID:855427"
CDS 87897..88736
/gene="PCL1"
/locus_tag="YNL289W"
/gene_synonym="HCS26"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:14688790]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14688790]"
/experiment="EXISTENCE:direct assay:GO:0016538
cyclin-dependent protein serine/threonine kinase regulator
activity [PMID:7973730]"
/experiment="EXISTENCE:genetic interaction:GO:0031106
septin ring organization [PMID:19458192]"
/experiment="EXISTENCE:genetic interaction:GO:0032878
regulation of establishment or maintenance of cell
polarity [PMID:17853895]"
/experiment="EXISTENCE:mutant phenotype:GO:0016239
positive regulation of macroautophagy [PMID:20417603]"
/experiment="EXISTENCE:physical interaction:GO:0000307
cyclin-dependent protein kinase holoenzyme complex
[PMID:7973730]"
/experiment="EXISTENCE:physical interaction:GO:0032878
regulation of establishment or maintenance of cell
polarity [PMID:17853895]"
/note="Cyclin, interacts with cyclin-dependent kinase
Pho85p; member of the Pcl1,2-like subfamily, involved in
the regulation of polarized growth and morphogenesis and
progression through the cell cycle; is ubiquitinated by
Dma1p; phosphorylation by Pho85p targets it for
degradation; localizes to sites of polarized cell growth"
/codon_start=1
/product="Pcl1p"
/protein_id="NP_014110.1"
/db_xref="GeneID:855427"
/db_xref="SGD:S000005233"
/translation="MCEYSKALHILLKSPVTDDIIKFLTDTTLRVVPSSNYPTPPGSP
GEKHLTRLPSLMTFITRLVRYTNVYTPTLLTAACYLNKLKRILPRDATGLPSTIHRIF
LACLILSAKFHNDSSPLNKHWARYTDGLFTLEDINLMERQLLQLLNWDLRVNTEDLIL
DLQPLLEPIKQDLARSSDQRKRINMMMSMNRRTCAGTSPIRSNNRFKLYEKQRNVSIA
SDLSSATLVDSCNDLRRLKDVTNIANNTVANTNYVRTVEKWNDNVNRQSWDLEQIMSQ
HGF"
gene 89210..89306
/gene="SNR40"
/locus_tag="YNCN0001W"
/db_xref="GeneID:9164969"
ncRNA 89210..89306
/ncRNA_class="snoRNA"
/gene="SNR40"
/locus_tag="YNCN0001W"
/product="SNR40"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:8674114|PMID:10024243]"
/experiment="EXISTENCE:curator inference:GO:0031428 box
C/D methylation guide snoRNP complex
[PMID:10024243|PMID:8674114]"
/experiment="EXISTENCE:direct assay:GO:0000452 snoRNA
guided rRNA 2'-O-methylation [PMID:25653162]"
/experiment="EXISTENCE:mutant phenotype:GO:0030562 rRNA
2'-O-ribose methylation guide activity [PMID:10024243]"
/experiment="EXISTENCE:mutant phenotype:GO:0031167 rRNA
methylation [PMID:10024243]"
/note="C/D box small nucleolar RNA (snoRNA); guides
2'-O-methylation of large subunit (LSU) rRNA at position
U898 and small subunit (SSU) rRNA at position G1271"
/transcript_id="NR_132249.1"
/db_xref="GeneID:9164969"
/db_xref="SGD:S000007304"
rep_origin 89531..89804
/note="ARS1407; Autonomously Replicating Sequence"
/db_xref="SGD:S000118384"
gene <90303..>91424
/gene="CAF40"
/locus_tag="YNL288W"
/db_xref="GeneID:855428"
mRNA <90303..>91424
/gene="CAF40"
/locus_tag="YNL288W"
/product="CCR4-NOT core subunit CAF40"
/transcript_id="NM_001183126.1"
/db_xref="GeneID:855428"
CDS 90303..91424
/gene="CAF40"
/locus_tag="YNL288W"
/experiment="EXISTENCE:direct assay:GO:0032968 positive
regulation of transcription elongation by RNA polymerase
II [PMID:21406554]"
/experiment="EXISTENCE:physical interaction:GO:0030015
CCR4-NOT core complex [PMID:11733989]"
/note="Component of the CCR4-NOT transcriptional complex;
evolutionarily conserved; involved in controlling mRNA
initiation, elongation, and degradation; binds Cdc39p"
/codon_start=1
/product="CCR4-NOT core subunit CAF40"
/protein_id="NP_014111.1"
/db_xref="GeneID:855428"
/db_xref="SGD:S000005232"
/translation="MFSAQKPIYGNGAGVNMGGGGPSTNNPGSMSMPGVPTSMGPGMN
QQIPSGGPMLMGNTPNNNNSNENGENNGNNGNNGGNDANATRNNPNMVNNRGAVHALD
DPNVYHWICQLTYGPQKEQALLELGRKREQFDDLAVVLWSSFGVMTSLLNEIISVYPM
LQPQMLSNNLSNRVCNALVLLQCVASHPETKHLFLQAHIPLFLFPFLNTTSRQRTFEY
LRLTSLGVIGALVKNDSQDVITFLLRTDIVPLCLRIMESSSELSKTVAIFILQKILLD
DVGLQYICATLERFYAVTNVLKDMVEHLTVSTPPGRLLKHIIRCYLRLSDDLEARRLL
KIVLPAKLRDNTFTEVLRDDVGSKRCLAQLLLTLNEETS"
gene <91994..>94801
/gene="SEC21"
/locus_tag="YNL287W"
/db_xref="GeneID:855429"
mRNA <91994..>94801
/gene="SEC21"
/locus_tag="YNL287W"
/product="coatomer subunit gamma"
/transcript_id="NM_001183125.1"
/db_xref="GeneID:855429"
CDS 91994..94801
/gene="SEC21"
/locus_tag="YNL287W"
/experiment="EXISTENCE:direct assay:GO:0030126 COPI
vesicle coat [PMID:1461285]"
/experiment="EXISTENCE:direct assay:GO:0070971 endoplasmic
reticulum exit site [PMID:40699735]"
/experiment="EXISTENCE:mutant phenotype:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:1461285]"
/experiment="EXISTENCE:mutant phenotype:GO:0006890
retrograde vesicle-mediated transport, Golgi to
endoplasmic reticulum [PMID:8001155]"
/note="Gamma subunit of coatomer; coatomer is a heptameric
protein complex that together with Arf1p forms the COPI
coat; involved in ER to Golgi transport of selective
cargo"
/codon_start=1
/product="coatomer subunit gamma"
/protein_id="NP_014112.1"
/db_xref="GeneID:855429"
/db_xref="SGD:S000005231"
/translation="MSAHTYKKFENSTSGDLPDKMTIYQDCMNTFNESPVNSKRCRLL
ISRLLRLLAQGETFPQNEATALFFSISKLFQHQNDPLRQAVYLAIKELSGISEDVLMA
TSSIMKDVQNGSDLIKPDAIRSLTYVLDESTAFSAERLLKSAVVSRHPSISSAALCTS
YHLLPISEVTIRRFTNETQEAVLDLKQFPNQHGNSEYYPNSTYISQYHALGLLYQLKK
TDKMALLKLVRHFSENNSMKNQLAKVELVKIVNDLIYRDPQLFSQFRPLLSDWLSNKF
ESVQLETAKLITSFATRNSRLVAPELYAAAISALQSLLTVPRVSSRFAALRILNRISM
VSPEKIVVCNPELESLINDSNRNISTYAITTLLKTGTSKNISSLISTITNFIHDVSDD
FKIIIIDAVRTLSLNFPQEWKSILNFLIDVLKNSEGGFKFKNSIVEALIDIVSFVPQS
KELALENLCDFIEDCEFNEILVRILHLLGKEGPSAPNPSLYVRHIYNRVVLENSIIRS
AAVVALSKFALTKNDPTLYESIISLLKRIANDKDDEVRDRATIALEFIDSARNKDDVI
AQNLIESKYFYDIPSLESKLSSYISSNTDSFATAFDVNQVRKFTEDEMKAINLKRKQE
QIFNQKSETTLDTTPEAESVPEKRADANSFAGPNLDDHQEDLLATKYADELLSIEQIK
PFGQLVNSSRAISLTEPEAEFVVRGVKHLFKDNVVLQFNITNTLTDIALDNVSVVCTP
EISDEAELEELFTLQVDRLLPSEEAACYVAFKKLDEIVMEGFLNNLTFTTKEINPDTN
EPFDGDEGFQDEYEIDSIFLNAGDYVKSSFTGNFSATFDELPCEEVAVFNIQEDLSIQ
EVVDKIILNSSCLPVESTQFAPSDSNSHTLKLFGKSALTGSKVALQIKMIKSSKGLAL
KVHGKGEDSLLCSDLVNGLMQ"
gene <95223..>96080
/gene="CUS2"
/locus_tag="YNL286W"
/db_xref="GeneID:855430"
mRNA <95223..>96080
/gene="CUS2"
/locus_tag="YNL286W"
/product="U2 snRNP complex subunit CUS2"
/transcript_id="NM_001183124.1"
/db_xref="GeneID:855430"
CDS 95223..96080
/gene="CUS2"
/locus_tag="YNL286W"
/experiment="EXISTENCE:direct assay:GO:0030620 U2 snRNA
binding [PMID:26631165]"
/experiment="EXISTENCE:direct assay:GO:0034337 RNA folding
[PMID:26631165]"
/experiment="EXISTENCE:genetic interaction:GO:1903241
U2-type prespliceosome assembly [PMID:9710584]"
/experiment="EXISTENCE:mutant phenotype:GO:0030620 U2
snRNA binding [PMID:26631165|PMID:9710584]"
/experiment="EXISTENCE:mutant phenotype:GO:0034337 RNA
folding [PMID:26631165]"
/experiment="EXISTENCE:physical interaction:GO:0005686 U2
snRNP [PMID:26631165]"
/experiment="EXISTENCE:physical interaction:GO:1903241
U2-type prespliceosome assembly [PMID:9710584]"
/note="Putative checkpoint factor in transcription; binds
to U2 snRNA and Prp11p; regulates toggling of the U2 snRNA
stem II region between different structures; contains two
RNA recognition motifs (RRMs)"
/codon_start=1
/product="U2 snRNP complex subunit CUS2"
/protein_id="NP_014113.1"
/db_xref="GeneID:855430"
/db_xref="SGD:S000005230"
/translation="MDADELELKGHLKKLKKEELLRRKQLKESNLQKRELEYNNASKN
TSIYISGLPTDKTTKEGLTEQFCKYGMIRTNRDGEPLCKLYVNDKGAFKGDALITYSK
EESVTLAIEMMNESIFLGKQIRVERAQFQNKEGDNMHGKENDLKEFNGPEPPIKRLKK
AKSEGEGEVIDYNDDESLAKADRTVIFANVFNIYKSYTNDDINDIQEDLLEGCEEIGQ
VDSISVSPNKGEATVVFKNNKVALQCCKIMTGRYFDGQKLLAFISGDENTSSTSDKNE
DSEVEDDLI"
gene complement(96241..96312)
/gene="SUF6"
/locus_tag="YNCN0002C"
/db_xref="GeneID:855432"
tRNA complement(96241..96312)
/gene="SUF6"
/locus_tag="YNCN0002C"
/product="tRNA-Gly"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE
analysis; can mutate to suppress +1 frameshift mutations
in glycine codons"
/db_xref="GeneID:855432"
/db_xref="SGD:S000006591"
rep_origin 96247..96463
/note="ARS1409; Putative replication origin; identified in
multiple array studies, not yet confirmed by plasmid-based
assay"
/db_xref="SGD:S000130140"
repeat_region 96451..96609
/note="Ty4 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007140"
mobile_element complement(96610..102523)
/note="YNLCTy1-1; Ty1 element, LTR retrotransposon of the
Copia (Pseudoviridae) group; contains co-transcribed genes
TYA Gag and TYB Pol, encoding proteins involved in
structure and function of virus-like particles, flanked by
two direct repeats"
/mobile_element_type="retrotransposon:YNLCTy1-1"
/db_xref="SGD:S000007134"
repeat_region complement(96610..96941)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007129"
gene complement(<96964..>102232)
/locus_tag="YNL284C-B"
/db_xref="GeneID:855433"
mRNA complement(<96964..>102232)
/locus_tag="YNL284C-B"
/product="gag-pol fusion protein"
/transcript_id="NM_001184415.2"
/db_xref="GeneID:855433"
CDS complement(join(96964..100926,100928..102232))
/locus_tag="YNL284C-B"
/EC_number="2.7.7.7"
/EC_number="2.7.7.49"
/EC_number="3.1.26.4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9448009]"
/ribosomal_slippage
/note="Retrotransposon TYA Gag and TYB Pol genes;
transcribed/translated as one unit; polyprotein is
processed to make a nucleocapsid-like protein (Gag),
reverse transcriptase (RT), protease (PR), and integrase
(IN); similar to retroviral genes"
/codon_start=1
/product="gag-pol fusion protein"
/protein_id="NP_058178.1"
/db_xref="GeneID:855433"
/db_xref="SGD:S000007387"
/translation="MESQQLSQHSPISHGSACASVTSKEVHTNQDPLDVSASKIQEYD
KASTKANSQQTTTPASSAVPENPHHASPQTAQSHSPQNGPYQQQCMMTQNQANPSGWS
FYGRPSMIPYTPYQMSPMYFPPGPHSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFLNWVKTYIKFLQNSNLGDIIPTATRKAVRQMTDDELT
FLCHTFQLFAPSQFLPTWVKDILSADYTDIMKILSKSINKMQSDTQEVNDITTLATLH
YNGSTPADAFEAEVTNILDRLNNNGIPINNKVACQFIMRGLSGEYKFLRYARHRYIHM
TVADLFSDIHSMYEEQQESKRNKSTYRRNPSDEKKDSRTYTNTTKPKSITRNSQKPNN
SQSRTARAHNVSTSNNSSGPDNDLIRGSTTEPIQLKNKHDLHLGQELTESTVNHTNHS
DDELPGHLLLDSGASRTLIRSAHHIHSASSNPGINVVDAQKRNIPINAIGDLQFHFQD
NTKTSIKVLHTPNIAYDLLSLNELAAVDITACFTKNVLERSDGTVLAPIVKYGDFYWV
SKKYLLPSNISVPTINNVHTSESTRKYPYPFIHRMLAHANAQTIRYSLKNNTITYFNE
SDVDWSSAIDYQCPDCLIGKSTKHRHIKGSRLKYQNSYEPFQYLHTDIFGPVHNLPKS
APSYFISFTDETTKFRWVYPLHDRREDSILDVFTTILAFIKNQFQASVLVIQMDRGSE
YTNRTLHKFLEKNGITPCYTTTADSRAHGVAERLNRTLLDDCRTQLQCSGLPNHLWFS
AIEFSTIVRNSLASPKSKKSARQHAGLAGLDISTLLPFGQPVIVNDHNPNSKIHPRGI
PGYALHPSRNSYGYIIYLPSLKKTVDTTNYVILQGKESRLDQFNYDALTFDEDLNRLT
ASYQSFIASNEIQQSDDLNIESDHDFQSDIELHPEQPRNVLSKAVSPTDSTPPSTHTE
DSKRVSKTNIRAPREVDPNISKSNILPSKKRSSTPQISDIESTGSGGMHRLDVPLLAP
MSQYNTHESSHTSKSKDFRHSDSYSDNETNHTNVPISSTGGTNNKTVPQTSEQETEKR
IIHRSPSIDTSSSESNSLHHVVPIKTSDTCPKENTEESIIADLPLPDLPPEPPTKLSD
SFKELPPINSRQTNSSLGGIGDSNAYTTINSKKRSLEDNETEIKVSRDTWNTKNMRSL
EPPRSKKRIHLIAAVKAVKSIKPIRTTLRYDEAITYNKDIKEKEKYIEAYHKEVNQLL
KMKTWDTDRYYDRKEIDPKRVINSMFIFNRKRDGTHKARFVARGDIQHPDTYDSGMQS
NTVHHYALMTSLSLALDNNYYITQLDISSAYLYADIKEELYIRPPPHLGMNDKLIRLK
KSLYGLKQSGANWYETIKSYLIQQCGMEEVRGWSCVFKNSQVTICLFVDDMVLFSKNL
NSNKRIIEKLKMQYDTKIINLGESDEEIQYDILGLEIKYQRGKYMKLGMENSLTEKIP
KLNVPLNPKGRKLSAPGQPGLYIDQQELELEEDDYKMKVHEMQKLIGLASYVGYKFRF
DLLYYINTLAQHILFPSKQVLDMTYELIQFIWNTRDKQLIWHKSKPVKPTNKLVVISD
ASYGNQPYYKSQIGNIYLLNGKVIGGKSTKASLTCTSTTEAEIHAISESVPLLNNLSY
LIQELDKKPITKGLLTDSKSTISIIISNNEEKFRNRFFGTKAMRLRDEVSGNHLHVCY
IETKKNIADVMTKPLPIKTFKLLTNKWIH"
gene complement(<100910..>102232)
/locus_tag="YNL284C-A"
/db_xref="GeneID:855434"
mRNA complement(<100910..>102232)
/locus_tag="YNL284C-A"
/product="gag protein"
/transcript_id="NM_001184414.1"
/db_xref="GeneID:855434"
CDS complement(100910..102232)
/locus_tag="YNL284C-A"
/note="Retrotransposon TYA Gag gene co-transcribed with
TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is
a nucleocapsid protein that is the structural constituent
of virus-like particles (VLPs); similar to retroviral Gag"
/codon_start=1
/product="gag protein"
/protein_id="NP_058179.1"
/db_xref="GeneID:855434"
/db_xref="SGD:S000007386"
/translation="MESQQLSQHSPISHGSACASVTSKEVHTNQDPLDVSASKIQEYD
KASTKANSQQTTTPASSAVPENPHHASPQTAQSHSPQNGPYQQQCMMTQNQANPSGWS
FYGRPSMIPYTPYQMSPMYFPPGPHSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFLNWVKTYIKFLQNSNLGDIIPTATRKAVRQMTDDELT
FLCHTFQLFAPSQFLPTWVKDILSADYTDIMKILSKSINKMQSDTQEVNDITTLATLH
YNGSTPADAFEAEVTNILDRLNNNGIPINNKVACQFIMRGLSGEYKFLRYARHRYIHM
TVADLFSDIHSMYEEQQESKRNKSTYRRNPSDEKKDSRTYTNTTKPKSITRNSQKPNN
SQSRTARAHNVSTSNNSSGPDNDLIRGSTTEPIQLKNKHDLHLRPGTY"
repeat_region complement(102192..102523)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007130"
repeat_region complement(102632..102700)
/note="Ty3 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007133"
gene 102716..102789
/locus_tag="YNCN0003W"
/db_xref="GeneID:855435"
tRNA 102716..102789
/locus_tag="YNCN0003W"
/product="tRNA-Asn"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Asparagine tRNA (tRNA-Asn), predicted by
tRNAscan-SE analysis"
/db_xref="GeneID:855435"
/db_xref="SGD:S000006673"
gene complement(<103134..>104102)
/gene="MRPL10"
/locus_tag="YNL284C"
/gene_synonym="MRPL18; uL15m"
/db_xref="GeneID:855436"
mRNA complement(<103134..>104102)
/gene="MRPL10"
/locus_tag="YNL284C"
/gene_synonym="MRPL18; uL15m"
/product="mitochondrial 54S ribosomal protein uL15m
MRPL10"
/transcript_id="NM_001183122.1"
/db_xref="GeneID:855436"
CDS complement(103134..104102)
/gene="MRPL10"
/locus_tag="YNL284C"
/gene_synonym="MRPL18; uL15m"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:9151978]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:9151978]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005762
mitochondrial large ribosomal subunit [PMID:9151978]"
/note="Mitochondrial ribosomal protein of the large
subunit; appears as two protein spots (YmL10 and YmL18) on
two-dimensional SDS gels"
/codon_start=1
/product="mitochondrial 54S ribosomal protein uL15m
MRPL10"
/protein_id="NP_014115.1"
/db_xref="GeneID:855436"
/db_xref="SGD:S000005228"
/translation="MKAERQTGLRNSFTTVIGRKLINTFVPSMMLTSVAGNDIFFRGL
FKSPVLAFQSYRYVSILGQLKPSDGSTKSFKRLGRGPSSGLGKTSGRGQKGQKARGKV
KSWFEGGQTPIYKLFPKIGFTNVGAKPLKELNLKRIQWFHDKNRLHLQPGEVLDMNKM
RKLGLVTGPIKYGVKILASGKFHYNLPIALEASRASAKAIAAIEKAGGKFTARYYTPL
GLRAHLNPQWFLEKRGRVPLQARPTKRRDIDFYSKEEKRGYLVMEKDKLLQDIKEAQN
KGSRHFLKQNVKKSSLEIELEELSPEKDWVPVVSNSKVMNIKALDH"
gene 104805..104877
/locus_tag="YNCN0004W"
/db_xref="GeneID:855437"
tRNA 104805..104877
/locus_tag="YNCN0004W"
/product="tRNA-Thr"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:855437"
/db_xref="SGD:S000006744"
gene complement(<105184..>106695)
/gene="WSC2"
/locus_tag="YNL283C"
/gene_synonym="STA3"
/db_xref="GeneID:855438"
mRNA complement(<105184..>106695)
/gene="WSC2"
/locus_tag="YNL283C"
/gene_synonym="STA3"
/product="Wsc2p"
/transcript_id="NM_001183121.1"
/db_xref="GeneID:855438"
CDS complement(105184..106695)
/gene="WSC2"
/locus_tag="YNL283C"
/gene_synonym="STA3"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:20652590|PMID:27337501]"
/experiment="EXISTENCE:direct assay:GO:0005933 cellular
bud [PMID:20652590|PMID:13679573]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:20652590]"
/experiment="EXISTENCE:direct assay:GO:0030427 site of
polarized growth [PMID:11545731]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807]"
/experiment="EXISTENCE:mutant phenotype:GO:0004888
transmembrane signaling receptor activity [PMID:10430578]"
/experiment="EXISTENCE:mutant phenotype:GO:0007266 Rho
protein signal transduction [PMID:10348843]"
/experiment="EXISTENCE:mutant phenotype:GO:0009408
response to heat [PMID:10348843]"
/experiment="EXISTENCE:mutant phenotype:GO:0031505
fungal-type cell wall organization [PMID:10348843]"
/note="Sensor-transducer of the stress-activated PKC1-MPK1
signaling pathway; involved in maintenance of cell wall
integrity and recovery from heat shock; required for the
arrest of secretion response; WSC2 has a paralog, WSC3,
that arose from the whole genome duplication"
/codon_start=1
/product="Wsc2p"
/protein_id="NP_014116.1"
/db_xref="GeneID:855438"
/db_xref="SGD:S000005227"
/translation="MHLDLIHKSFILVWLIYIRAALADQFTYKACYSASDIRKLGLTY
KGVYEYQSVSYCQNECPGQAVVALFNGTGCYCGGSVAQLQSLTQVDSSKCDVSCAGWP
YQNCGGSSAMNVYINNAASTADSTSSTATSTSTTSSSSTSVSSKTSTKLDTKTSTSSS
ATHSSSSSSTTSTTTSSSETTTSSSSSSSSSSTSTTSTTSTTSSTTSTSSSPSTTSSS
TSASSSSETSSTQATSSSTTSTSSSTSTATVTSTPSSTSIGTSTHYTTRVVTQSVVSQ
ANQQASTIFTTRTSVYATVSSTSSSTSSLLNGKSSSSKSKGLSGGAIAGVVVGVVCGT
VALLALALFFFVWKKRRQSSQHVDLEETKQYQPYSLGDADANPVIPPSASSTNWHIPS
RNNTALSKNTASTFATYDLPTRAPGGRDSIITGDAHNISKRSHFPSVVYEEPPSIYNG
NQRFSATSLPDMMEERQLHIVNPDNVSSNIGSNVSDGDDDYDDAKDSNNSSLR"
gene <107687..>108274
/gene="POP3"
/locus_tag="YNL282W"
/db_xref="GeneID:855439"
mRNA <107687..>108274
/gene="POP3"
/locus_tag="YNL282W"
/product="Pop3p"
/transcript_id="NM_001183120.1"
/db_xref="GeneID:855439"
CDS 107687..108274
/gene="POP3"
/locus_tag="YNL282W"
/experiment="EXISTENCE:direct assay:GO:0000172
ribonuclease MRP complex [PMID:9620854]"
/experiment="EXISTENCE:direct assay:GO:0000294
nuclear-transcribed mRNA catabolic process, RNase
MRP-dependent [PMID:14729943]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005655 nucleolar
ribonuclease P complex [PMID:9620854]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0034965 intronic
box C/D snoRNA processing [PMID:18713869]"
/experiment="EXISTENCE:mutant phenotype:GO:0000171
ribonuclease MRP activity [PMID:9029160]"
/experiment="EXISTENCE:mutant phenotype:GO:0004526
ribonuclease P activity [PMID:9029160]"
/experiment="EXISTENCE:mutant phenotype:GO:0006364 rRNA
processing [PMID:9029160]"
/experiment="EXISTENCE:mutant phenotype:GO:0008033 tRNA
processing [PMID:9029160]"
/experiment="EXISTENCE:physical interaction:GO:0000172
ribonuclease MRP complex [PMID:17881380|PMID:9029160]"
/experiment="EXISTENCE:physical interaction:GO:0005655
nucleolar ribonuclease P complex [PMID:9029160]"
/note="Subunit of both RNase MRP and nuclear RNase P;
RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves
tRNA precursors to generate mature 5' ends and facilitates
turnover of nuclear RNAs; increases the activity and
thermal stability of the RNase P complex, along with
Pop2p; relocalizes to the cytosol in response to hypoxia"
/codon_start=1
/product="Pop3p"
/protein_id="NP_014117.1"
/db_xref="GeneID:855439"
/db_xref="SGD:S000005226"
/translation="MSGSLKSLDKKIAKRRQVYKPVLDNPFTNEAHMWPRVHDQPLIW
QLLQSSIINKLIHIQSKENYPWELYTDFNEIVQYLSGAHGNSDPVCLFVCNKDPDVPL
VLLQQIPLLCYMAPMTVKLVQLPKSAMDTFKSVSKYGMLLLRCDDRVDKKFVSQIQKN
VDLLQFPWLNAIKYRPTSVKLLKTTVPIVSKKRQK"
gene <108467..>108928
/gene="HCH1"
/locus_tag="YNL281W"
/db_xref="GeneID:855440"
mRNA <108467..>108928
/gene="HCH1"
/locus_tag="YNL281W"
/product="Hch1p"
/transcript_id="NM_001183119.1"
/db_xref="GeneID:855440"
CDS 108467..108928
/gene="HCH1"
/locus_tag="YNL281W"
/experiment="EXISTENCE:direct assay:GO:0001671 ATPase
activator activity [PMID:12504007]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0051087
protein-folding chaperone binding [PMID:12504007]"
/experiment="EXISTENCE:mutant phenotype:GO:0006457 protein
folding [PMID:12504007]"
/experiment="EXISTENCE:physical interaction:GO:0006457
protein folding [PMID:12504007]"
/note="Heat shock protein regulator; binds to Hsp90p and
may stimulate ATPase activity; originally identified as a
high-copy number suppressor of a HSP90 loss-of-function
mutation; role in regulating Hsp90 inhibitor drug
sensitivity; GFP-fusion protein localizes to the cytoplasm
and nucleus; protein abundance increases in response to
DNA replication stress"
/codon_start=1
/product="Hch1p"
/protein_id="NP_014118.1"
/db_xref="GeneID:855440"
/db_xref="SGD:S000005225"
/translation="MVVLNPNNWHWVDKNTLPWSKDYLNGKLTSLSTVSSDGKSKIEL
TQVSSITGDSNVSQRKGKPICYFDLQLSMNVKVTNLDTNKDDEDDDGILADGKLEIPE
FMHDESDIPILSQGFDAFDGLVRSEFVPKVVETLLKYQDDLIKEHSKDIQV"
gene complement(<109095..>110411)
/gene="ERG24"
/locus_tag="YNL280C"
/db_xref="GeneID:855441"
mRNA complement(<109095..>110411)
/gene="ERG24"
/locus_tag="YNL280C"
/product="delta(14)-sterol reductase"
/transcript_id="NM_001183118.1"
/db_xref="GeneID:855441"
CDS complement(109095..110411)
/gene="ERG24"
/locus_tag="YNL280C"
/EC_number="1.3.1.70"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0050613
Delta14-sterol reductase activity [PMID:32908]"
/experiment="EXISTENCE:mutant phenotype:GO:0006696
ergosterol biosynthetic process
[PMID:8125337|PMID:1394506|PMID:1418625]"
/experiment="EXISTENCE:mutant phenotype:GO:0050613
Delta14-sterol reductase activity
[PMID:8125337|PMID:1394506]"
/note="C-14 sterol reductase; acts in ergosterol
biosynthesis; mutants accumulate the abnormal sterol
ignosterol (ergosta-8,14 dienol), and are viable under
anaerobic growth conditions but inviable on rich medium
under aerobic conditions"
/codon_start=1
/product="delta(14)-sterol reductase"
/protein_id="NP_014119.1"
/db_xref="GeneID:855441"
/db_xref="SGD:S000005224"
/translation="MVSALNPRTTEFEFGGLIGALGISIGLPVFTIILNQMIRPDYFI
KGFFQNFDIVELWNGIKPLRYYLGNRELWTVYCLWYGILAVLDVILPGRVMKGVQLRD
GSKLSYKINGIAMSTTLVLVLAIRWKLTDGQLPELQYLYENHVSLCIISILFSFFLAT
YCYVASFIPLIFKKNGNGKREKILALGGNSGNIIYDWFIGRELNPRLGPLDIKMFSEL
RPGMLLWLLINLSCLHHHYLKTGKINDALVLVNFLQGFYIFDGVLNEEGVLTMMDITT
DGFGFMLAFGDLSLVPFTYSLQARYLSVSPVELGWVKVVGILAIMFLGFHIFHSANKQ
KSEFRQGKLENLKSIQTKRGTKLLCDGWWAKSQHINYFGDWLISLSWCLATWFQTPLT
YYYSLYFATLLLHRQQRDEHKCRLKYGENWEEYERKVPYKIIPYVY"
gene <110917..>112902
/gene="PRM1"
/locus_tag="YNL279W"
/db_xref="GeneID:855442"
mRNA <110917..>112902
/gene="PRM1"
/locus_tag="YNL279W"
/product="pheromone-regulated protein PRM1"
/transcript_id="NM_001183117.1"
/db_xref="GeneID:855442"
CDS 110917..112902
/gene="PRM1"
/locus_tag="YNL279W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:11062271]"
/experiment="EXISTENCE:mutant phenotype:GO:0032220 plasma
membrane fusion involved in cytogamy
[PMID:11062271|PMID:15590839]"
/note="Pheromone-regulated multispanning membrane protein;
involved in plasma membrane fusion during mating; contains
four transmembrane domains and two large extracellular
loops; functions as a disulfide-linked homodimer;
localizes to the shmoo tip and to a central plaque at the
prezygotic zone of cell fusion; regulated by Ste12p"
/codon_start=1
/product="pheromone-regulated protein PRM1"
/protein_id="NP_014120.1"
/db_xref="GeneID:855442"
/db_xref="SGD:S000005223"
/translation="MSGFKCYLQLGDRLSQIWLNKYTLVLLLAMLKLLFFSKSIQHAI
EVSETYILSNCYSIDSLYSKMTDNTPHYLGIMGNYLIEKGMEETVKATLETLSLIVYA
SEGLVNFAIDLYLGTYACLIVSAVDGTVDVATNITEKLISLVNDTVSSVANELDTGLN
DISKIINKVIKAASKVENFFTGDDDDSNMTSSIKSVNLTISALHNLYIPSSINDKLEE
LSAKTPDFAQVKNTTKNLISVPFNEVRKNIKAVNASNIIGDTSVLYVPPVSLDNSTGI
CSSNQSEILAFYSILGHVLKIATVVCITVLICFAVGAMAPVAWNEIKLWRRLCGMRDH
YMLSRQDSYTSFSSENTHELKDPFRDPPIQNGQYDVIASYQQCFQTWNTRIAGWMTNL
VTFGKSPENIDPKTKQKIEWVVAYMTSERALCVLGIGLLGILVCICQFVMIALLKHKI
SHSLTSNDGDGVQNLLKSSTAVDIENQMSLWSVQTNKYINTTETNINQEVFGWINTTT
LSVNNTVATMISDIDTTLADVFNGTLLYNPMKTVVGCAIENKLYTIEKAMTWIHDKAQ
LHIPRINGTQIKQALAKQTDNSTIPTASSTSAATENLLENLVNDMREGLLKILRAYHR
ITLGELTVALVILAVWLVQLPIALVILRLRLRKATFD"
gene <113271..>116453
/gene="CAF120"
/locus_tag="YNL278W"
/db_xref="GeneID:855443"
mRNA <113271..>116453
/gene="CAF120"
/locus_tag="YNL278W"
/product="Caf120p"
/transcript_id="NM_001183116.1"
/db_xref="GeneID:855443"
CDS 113271..116453
/gene="CAF120"
/locus_tag="YNL278W"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:25028499]"
/note="Part of the CCR4-NOT transcriptional regulatory
complex; involved in controlling mRNA initiation,
elongation, and degradation; contains a PH-like domain;
CAF120 has a paralog, SKG3, that arose from the whole
genome duplication"
/codon_start=1
/product="Caf120p"
/protein_id="NP_014121.1"
/db_xref="GeneID:855443"
/db_xref="SGD:S000005222"
/translation="MRIFSGDNKVVDSLASNPGLMSPSNFGGDFGSRLKVNVTSKKKL
NDSSPTSPMESSPVSPELVPILTLLNAHTHRRYHEGVFLILQDLNNNGTHAARKWKDV
YGVLLGTQLALWDAKELAEFTDPSCPVSEKKLKEVASKPTYINLTDATLRTLDNSDNI
VMECGKNLTNALVVSTTLKNRYFLQFGNKESFNAWNSAIRLCLYECSSLQEAYTGAFI
SSRGAKLGDIRILLTNRKYDYKDWVSVRFGAGMPWKRCYAVISQSSSKKKGHFGEINL
YENDKKVKKNHAMATIVEAKALYAVYPSSPKLIDSSTIIKVVGSVKFEKKESAQEKDV
FIMPEKHQAVPSYDTIIRFLIPAMDTFKLYGRPEKLLSSKNDPHSLLFGLPVLPHIYY
LEVEDLLPLTNSVSSLHWSNNEWKEHISDILQRKIAQGYCGCNSTSNITSPLPSPFLG
SADLFERADGVLSPKLSYGSKSSSNNSSKNSLPKRERVKLSSSSEQDLNNSDSPSIKR
KSPPLVISESPHKVHTPTDASFRTRVTEGSPYAKQRHPKPFASSVNDSPSDRAKSRTV
PYNNNDRKATTPEKFERGETSCGKNVDESLEKVRNMKLEIPESNFDKFMTDKNLLSVD
SKCSNEKKLSVESDLSAIYEKYSNGPFGHTEGLNGSSDETYLRFQRASVHSESNYNSR
KSFTPSDFSNGNEEEHAVLQELNSLTQRINELGMESINSNSDSDRINGSYSQVDFGNN
NDEDDMNLFDPDFMAQDQLRAEERDYNKDDRTPLAKVPAAFQSTGLGITPDDDIERQY
ITEHRSRHEVPKRSPEKPSNPLEIGNPYAKPGTRLNTTHTHSKTDRSITPQRGQPVPS
GQQISSYVQPANINSPNKMYGANNSAMGSPRNPKTRAPPGPYNQGWNNRPSPSNIYQR
PHPSDTQPQAYHLPGNPYSTGNRPNMQAQYHPQQVPMPILQQPNRPYQPYAMNTHMGS
PGGYAGAAPPFQPANVNYNTRPQQPWPTPNSPSAHYRPPPNLNQPQNGSAGYYRPPAP
QLQNSQARPQKKDGFSQFMPSATTKNPYAQ"
gene <116679..>116867
/locus_tag="YNL277W-A"
/db_xref="GeneID:1466509"
mRNA <116679..>116867
/locus_tag="YNL277W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184664.1"
/db_xref="GeneID:1466509"
CDS 116679..116867
/locus_tag="YNL277W-A"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878151.1"
/db_xref="GeneID:1466509"
/db_xref="SGD:S000028852"
/translation="MTLAYYGQPVKMCHILPPLRSLPVLVGKKKLKKKKSQTTNNHVI
FLFTLFIKLLKTHNRMSL"
gene <117349..>118809
/gene="MET2"
/locus_tag="YNL277W"
/db_xref="GeneID:855444"
mRNA <117349..>118809
/gene="MET2"
/locus_tag="YNL277W"
/product="homoserine O-acetyltransferase"
/transcript_id="NM_001183115.1"
/db_xref="GeneID:855444"
CDS 117349..118809
/gene="MET2"
/locus_tag="YNL277W"
/EC_number="2.3.1.31"
/experiment="EXISTENCE:direct assay:GO:0004414 homoserine
O-acetyltransferase activity [PMID:3301801]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0009092 homoserine
metabolic process [PMID:3301801]"
/experiment="EXISTENCE:mutant phenotype:GO:0000096 sulfur
amino acid metabolic process [PMID:1101032]"
/experiment="EXISTENCE:mutant phenotype:GO:0004414
homoserine O-acetyltransferase activity [PMID:3301801]"
/experiment="EXISTENCE:mutant phenotype:GO:0009086
methionine biosynthetic process [PMID:6035500]"
/experiment="EXISTENCE:mutant phenotype:GO:0009092
homoserine metabolic process [PMID:3301801]"
/note="L-homoserine-O-acetyltransferase; catalyzes the
conversion of homoserine to O-acetyl homoserine which is
the first step of the methionine biosynthetic pathway"
/codon_start=1
/product="homoserine O-acetyltransferase"
/protein_id="NP_014122.1"
/db_xref="GeneID:855444"
/db_xref="SGD:S000005221"
/translation="MSHTLKSKTLQELDIEEIKETNPLLKLVQGQRIVQVPELVLESG
VVINNFPIAYKTWGTLNEAGDNVLVICHALTGSADVADWWGPLLGNDLAFDPSRFFII
CLNSMGSPYGSFSPLTINEETGVRYGPEFPLCTVRDDVRAHRIVLDSLGVKSIACVIG
GSMGGMLSLEWAAMYGKEYVKNMVALATSARHSAWCISWSEAQRQSIYSDPNYLDGYY
PVEEQPVAGLSAARMSALLTYRTRNSFENKFSRRSPSIAQQQKAQREETRKPSTVSEH
SLQIHNDGYKTKASTAIAGISGQKGQSVVSTASSSDSLNSSTSMTSVSSVTGEVKDIK
PAQTYFSAQSYLRYQGTKFINRFDANCYIAITRKLDTHDLARDRVDDITEVLSTIQQP
SLIIGIQSDGLFTYSEQEFLAEHIPKSQLEKIESPEGHDAFLLEFKLINKLIVQFLKT
NCKAITDAAPRAWGGDVGNDETKTSVFGEAEEVTNW"
gene <119268..>120998
/gene="BOR1"
/locus_tag="YNL275W"
/db_xref="GeneID:855446"
mRNA <119268..>120998
/gene="BOR1"
/locus_tag="YNL275W"
/product="Bor1p"
/transcript_id="NM_001183113.1"
/db_xref="GeneID:855446"
CDS 119268..120998
/gene="BOR1"
/locus_tag="YNL275W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:16565073]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:11401825|PMID:12447444]"
/experiment="EXISTENCE:direct assay:GO:0046713 borate
transport [PMID:17459946]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0080139 borate
efflux transmembrane transporter activity [PMID:17459946]"
/experiment="EXISTENCE:mutant phenotype:GO:0006623 protein
targeting to vacuole [PMID:16565073]"
/experiment="EXISTENCE:mutant phenotype:GO:0046713 borate
transport [PMID:17459946|PMID:12447444|PMID:16923078]"
/experiment="EXISTENCE:mutant phenotype:GO:0080139 borate
efflux transmembrane transporter activity
[PMID:17459946|PMID:12447444|PMID:16923078]"
/note="Boron efflux transporter of the plasma membrane;
binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the
characterized boron efflux transporter A. thaliana BOR1;
human ortholog SLC4A5 is key biomarker and therapeutic
target for Niemann-Pick type C (NPC) disease"
/codon_start=1
/product="Bor1p"
/protein_id="NP_014124.1"
/db_xref="GeneID:855446"
/db_xref="SGD:S000005219"
/translation="MSNESTRVTVSRGCTASDECAQALERTNDELDRESSVSESRSDE
ESHEKLSRRRFPTLGIGIWLDLKDRIPYYKSDWVDAFNYRVIPSIVDTYFNNLLPAIA
FAQDMFDRTDNSYGVNEVLLSSAMAGIVFGVLGGQPLCIVGVTGPISIFNYTVYEIIK
PLNTSYFGFMFWICMWSMIFHLVLAFTNAVCLLQYVTTFPCDIFGLFINVVYIQKGIQ
ILTRQFSAKSGEKSVQDGFASVVVALVMTAFGLFFKLFHYYPLFSHRIRTFISDYSTA
LSVLFWSSFTHFGGYLHDVKFKKLPITKAFFPTSKVNRPQNTWLAYEPIPVKDVFIAL
PFGIFLTILFYFDHNVSSLMAQRHQYKLKKPSSFHYDFALLGLTTCISGVLGIPAPNG
LIPQAPLHTETLLVRDSNQKVISCVEQRFTNTFQGLMILGTMTRPLLVCLGEIPQAVL
SGLFFIMGINGLMTNSIIQRLVFLFSDPNRRDNTSPLMKVSKKSMLIFLSFSLTGFAG
EFAITNTIAAIGFPLVLLLSVLVSFSFAYIFPTEELKILDTNVAQKFTIKNLLLENIR
DAKFCDKHED"
gene complement(<121118..>122170)
/gene="GOR1"
/locus_tag="YNL274C"
/db_xref="GeneID:855447"
mRNA complement(<121118..>122170)
/gene="GOR1"
/locus_tag="YNL274C"
/product="glyoxylate reductase"
/transcript_id="NM_001183112.1"
/db_xref="GeneID:855447"
CDS complement(121118..122170)
/gene="GOR1"
/locus_tag="YNL274C"
/EC_number="1.1.1.26"
/EC_number="1.1.1.79"
/EC_number="1.1.1.81"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:mutant phenotype:GO:0009436
glyoxylate catabolic process [PMID:17173333]"
/experiment="EXISTENCE:mutant phenotype:GO:0047964
glyoxylate reductase (NADH) activity [PMID:17173333]"
/note="Glyoxylate reductase; null mutation results in
increased biomass after diauxic shift; the authentic,
non-tagged protein is detected in highly purified
mitochondria in high-throughput studies; protein abundance
increases in response to DNA replication stress"
/codon_start=1
/product="glyoxylate reductase"
/protein_id="NP_014125.1"
/db_xref="GeneID:855447"
/db_xref="SGD:S000005218"
/translation="MSKKPIVLKLGKDAFGDQAWGELEKIADVITIPESTTREQFLRE
VKDPQNKLSQVQVITRTARSVKNTGRFDEELALALPSSVVAVCHTGAGYDQIDVEPFK
KRHIQVANVPDLVSNATADTHVFLLLGALRNFGIGNRRLIEGNWPEAGPACGSPFGYD
PEGKTVGILGLGRIGRCILERLKPFGFENFIYHNRHQLPSEEEHGCEYVGFEEFLKRS
DIVSVNVPLNHNTHHLINAETIEKMKDGVVIVNTARGAVIDEQAMTDALRSGKIRSAG
LDVFEYEPKISKELLSMSQVLGLPHMGTHSVETRKKMEELVVENAKNVILTGKVLTIV
PELQNEDWPNESKPLV"
gene <122883..>126599
/gene="TOF1"
/locus_tag="YNL273W"
/db_xref="GeneID:855448"
mRNA <122883..>126599
/gene="TOF1"
/locus_tag="YNL273W"
/product="Tof1p"
/transcript_id="NM_001183111.1"
/db_xref="GeneID:855448"
CDS 122883..126599
/gene="TOF1"
/locus_tag="YNL273W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0031298 replication
fork protection complex [PMID:19819872]"
/experiment="EXISTENCE:direct assay:GO:0043596 nuclear
replication fork [PMID:16103218]"
/experiment="EXISTENCE:genetic interaction:GO:0006281 DNA
repair [PMID:16219777]"
/experiment="EXISTENCE:genetic interaction:GO:0007064
mitotic sister chromatid cohesion
[PMID:17483413|PMID:14742714]"
/experiment="EXISTENCE:mutant phenotype:GO:0000076 DNA
replication checkpoint signaling [PMID:12944972]"
/experiment="EXISTENCE:mutant phenotype:GO:0006260 DNA
replication [PMID:16137625]"
/experiment="EXISTENCE:mutant phenotype:GO:0006281 DNA
repair [PMID:16219777]"
/experiment="EXISTENCE:mutant phenotype:GO:0007064 mitotic
sister chromatid cohesion [PMID:17483413|PMID:14742714]"
/experiment="EXISTENCE:mutant phenotype:GO:0031297
replication fork processing [PMID:18632578]"
/experiment="EXISTENCE:mutant phenotype:GO:0043111
replication fork arrest
[PMID:16418273|PMID:17652453|PMID:16103218]"
/experiment="EXISTENCE:mutant phenotype:GO:0043570
maintenance of DNA repeat elements [PMID:18321795]"
/note="Subunit of a replication-pausing checkpoint
complex; the replication fork protection complex
(Tof1p-Mrc1p-Csm3p) acts at stalled forks and is required
for rapid replication fork progression, replisome
stabilization under stress, mediating checkpoint signaling
and promoting sister chromatid cohesion after damage,
facilitating gap repair of damaged DNA; interacts with the
MCM helicase; recruits Top1p to the fork to suppress DNA
damage; relocalizes to the cytosol in response to hypoxia
to hypoxia"
/codon_start=1
/product="Tof1p"
/protein_id="NP_014126.1"
/db_xref="GeneID:855448"
/db_xref="SGD:S000005217"
/translation="MSADLQQGTTNAADFSLTVLRARIALLATAIGGPDYTSQIDPPP
YKLGDDCLACLKDLKRWFKLVDDQQKRWDVAMAVAEYRILTDDLLPILIDWENKCSLA
AKLAKNNPDHEEFRNKAYYDKIALNCLQLLVLMTWPLIVTEQSSSNQITLYGELKKHQ
LVYKKTILSMESGKVLRAAIRLALDVIKIDRLSRTPRDNMVLKLVLNFFRNVIAIEPG
EFTINTKKSMPKKGITSIDTLPPNVSMDDISLNTVISSFHKNKVFGFLLTLTSSLSKE
FDQDFINIPLLEIMFYFTKDVNQELLFPRQFETGTHSKVVNKNESSSANNIVTSAGFE
LSKLLQKEHQMRKNVIKHTSARHSRFGGLLSIQTPDKTRLTVSGSQALVDEKIALQKL
DDSKKWNKRIIKKHQSVAAEGLPNSLLNSQTGKAIFFTESNGKHFKEFINNFIDSGFN
ILLHSVTNYFTTEQDRMVTLEQVEYLLFFAWFVKYQLLRSKIDNSADIKQVSEALKEV
TFILVSSLLRSAYDLKNWTVTHAGMIAFNELLNLVSRTKAAQEEDSTDIEFIVSRLFS
DERIQLLSNLPKIGSKYSLQFMKSCIELTHSVLKVLEQYSDDKTLVIEGKSRRQKKFN
ISEGDITKLIEEENVDRDEALDILTSSLRSIEVNFQKVQANYMTEPVIETYINFLERF
RELEDDSIKKVFSFFHRVFVQAKEQALLFRFDLIILLREMLSPDGLDRMSRSRKYVSQ
FSDYFLARLKKRLKKSPAWFVGLLFPPLHNSEVGFYQRYGEYNVLNNESMYAAPASQF
KPIPDEEALPPSILLDMKYGVLVSTLLDDGKTELLDQLLKHITHTLDIFKSWLTVNVN
AGKETVNPPNEYFTLTGVLNNDPIFKDKDYRALLLLIGYSIPRKINEPCFLPGTVEVS
DLTVSCELVKKYLSTPFETPNGLPSSSYLLRVRSEKDSFSHNEQDGWEGDDDYDYNDP
YIVPDDQILSKSDAAYFKDLDNNASDKLKGTKFSKGIARSKKKDKRKRRKGEAKTNLP
MFGDQDDERPQTVRERHGVFSKEFISDSEDDEDLMNPIFFENETYMRWLLDKNNGQLT
EDRYIQFAKFAAERMNNGGVVTGDYTSLFGGSIPSIESIRATESSSFAPDKSLISLAS
HVASEMSIFDVNNNNNNQLSDDDVNSESRNSLGSSQPSNSQNMFQSEVYSRKESTKRS
LEASAADESDEDEEAIRLFGKKSRVVLSQGDSDD"
rep_origin 126599..126805
/note="ARS1410; Replication origin; identified in multiple
array studies, confirmed by plasmid-based recombinational
ARS assay"
/db_xref="SGD:S000130141"
gene complement(<126805..>129084)
/gene="SEC2"
/locus_tag="YNL272C"
/db_xref="GeneID:855449"
mRNA complement(<126805..>129084)
/gene="SEC2"
/locus_tag="YNL272C"
/product="guanine nucleotide exchange factor SEC2"
/transcript_id="NM_001183110.1"
/db_xref="GeneID:855449"
CDS complement(126805..129084)
/gene="SEC2"
/locus_tag="YNL272C"
/experiment="EXISTENCE:direct assay:GO:0005085
guanyl-nucleotide exchange factor activity [PMID:9199166]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:12045183]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:20493815]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:20493815|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0030133 transport
vesicle [PMID:12045183]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807]"
/experiment="EXISTENCE:direct assay:GO:0070273
phosphatidylinositol-4-phosphate binding [PMID:20493815]"
/experiment="EXISTENCE:genetic interaction:GO:0006914
autophagy [PMID:20444978]"
/experiment="EXISTENCE:mutant phenotype:GO:0006887
exocytosis [PMID:12045183]"
/experiment="EXISTENCE:mutant phenotype:GO:0006914
autophagy [PMID:20444978]"
/experiment="EXISTENCE:mutant phenotype:GO:0030435
sporulation resulting in formation of a cellular spore
[PMID:29293994]"
/experiment="EXISTENCE:mutant phenotype:GO:0032120
ascospore-type prospore membrane formation
[PMID:29293994]"
/experiment="EXISTENCE:mutant phenotype:GO:0070273
phosphatidylinositol-4-phosphate binding [PMID:20493815]"
/note="Guanyl-nucleotide exchange factor for the small
G-protein Sec4p; essential for post-Golgi vesicle
transport and for autophagy; associates with the exocyst,
via exocyst subunit Sec15p, on secretory vesicles"
/codon_start=1
/product="guanine nucleotide exchange factor SEC2"
/protein_id="NP_014127.1"
/db_xref="GeneID:855449"
/db_xref="SGD:S000005216"
/translation="MDASEEAKRVSIQVTSLSTQLIESVDKQSHLEEQLNKSLKTIAS
QKAAIENYNQLKEDYNTLKRELSDRDDEVKRLREDIAKENELRTKAEEEADKLNKEVE
DLTASLFDEANNMVADARKEKYAIEILNKRLTEQLREKDTLLDTLTLQLKNLKKVMHS
LDNESTVTNNSNRYSTILSDSATSSSTSLNKVPTSYSLASQDIYSGIVYSPSISSIRY
DISLYNEFLKFVAALPRCENIKATSTESKLIRRLVNDEIQPILKIDNASGIGWLVKKT
LLSLIIDGLVVVEPLSGVNATYQIGYNSSSPAKQATSNMPKMFKFPLDSPPVAVHAAC
SFCGESRDDIIEHARMYILKTLHKTDDGKEQVTNTYPLCHWCLLKLRQTCEIFAFLRS
LKVGAWHLEKLTTQNITKEDLEKFSEVTKHTKRDGRVSSQDKKTKRLSFMAGLGINSS
TKNKPKMEIFSSETNAKPGQPTTNIQRAWLQLCKLRCILHWTHIGIWAVDDSISSKIG
PLVEDDSDEDQNDAISVRLQDKALWKQDAKRPFSSSSAEESQKSDAFDFESGDMENEI
TGESSSDESSSDGSSTDNSTADSSSEDESSLADSTTSSADSSSPESIDNGEGDDTVTK
DDKSSIKSANNNEENSDCGDKKGRSIIKKKAPQRKIQKKKLLQDLDDLEEQFREESAI
DQTEFENAESNVKQNISSKRASSGDENSKKDNNEKTLKTNLTIGDKTQEQIGENSPSS
GLHASSSNDDNFDDAQEQQ"
gene complement(<129522..>135383)
/gene="BNI1"
/locus_tag="YNL271C"
/gene_synonym="PPF3; SHE5"
/db_xref="GeneID:855450"
mRNA complement(<129522..>135383)
/gene="BNI1"
/locus_tag="YNL271C"
/gene_synonym="PPF3; SHE5"
/product="formin BNI1"
/transcript_id="NM_001183109.2"
/db_xref="GeneID:855450"
CDS complement(129522..135383)
/gene="BNI1"
/locus_tag="YNL271C"
/gene_synonym="PPF3; SHE5"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:11154270]"
/experiment="EXISTENCE:direct assay:GO:0000133 polarisome
[PMID:16166638|PMID:9571251]"
/experiment="EXISTENCE:direct assay:GO:0005522 profilin
binding [PMID:12134165|PMID:15923184]"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:38656798]"
/experiment="EXISTENCE:direct assay:GO:0005884 actin
filament [PMID:12052901]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:11154270]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:11154270]"
/experiment="EXISTENCE:direct assay:GO:0032153 cell
division site [PMID:26771880]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:38656798|PMID:9082982|PMID:19053807]"
/experiment="EXISTENCE:direct assay:GO:0045010 actin
nucleation [PMID:12052901]"
/experiment="EXISTENCE:direct assay:GO:0051016 barbed-end
actin filament capping [PMID:14561409]"
/experiment="EXISTENCE:direct assay:GO:0070649
formin-nucleated actin cable assembly [PMID:12134165]"
/experiment="EXISTENCE:genetic interaction:GO:0007118
budding cell apical bud growth [PMID:10866679]"
/experiment="EXISTENCE:genetic interaction:GO:0032956
regulation of actin cytoskeleton organization
[PMID:17237521]"
/experiment="EXISTENCE:genetic interaction:GO:0051017
actin filament bundle assembly [PMID:23671312]"
/experiment="EXISTENCE:genetic interaction:GO:0070649
formin-nucleated actin cable assembly [PMID:11740491]"
/experiment="EXISTENCE:genetic interaction:GO:0071474
cellular hyperosmotic response [PMID:17237521]"
/experiment="EXISTENCE:genetic interaction:GO:1903475
mitotic actomyosin contractile ring assembly
[PMID:12419188]"
/experiment="EXISTENCE:mutant phenotype:GO:0000132
establishment of mitotic spindle orientation
[PMID:10085293]"
/experiment="EXISTENCE:mutant phenotype:GO:0005522
profilin binding [PMID:15923184]"
/experiment="EXISTENCE:mutant phenotype:GO:0005934
cellular bud tip [PMID:16166638]"
/experiment="EXISTENCE:mutant phenotype:GO:0007118 budding
cell apical bud growth [PMID:10866679]"
/experiment="EXISTENCE:mutant phenotype:GO:0032880
regulation of protein localization
[PMID:10085294|PMID:16166638]"
/experiment="EXISTENCE:mutant phenotype:GO:0032956
regulation of actin cytoskeleton organization
[PMID:17237521]"
/experiment="EXISTENCE:mutant phenotype:GO:0051017 actin
filament bundle assembly [PMID:23671312]"
/experiment="EXISTENCE:mutant phenotype:GO:0070649
formin-nucleated actin cable assembly [PMID:11740491]"
/experiment="EXISTENCE:mutant phenotype:GO:0071474
cellular hyperosmotic response [PMID:17237521]"
/experiment="EXISTENCE:physical interaction:GO:0000133
polarisome [PMID:9571251]"
/note="Formin; polarisome component; nucleates the
formation of linear actin filaments, involved in budding
and mitotic spindle orientation which require the
formation of polarized actin cables; recruited to the
division site in a Glc7p/Ref2p dependent manner following
release of Bnr1p; spatially focuses Bni1p at mating
projection tips, and with Bud6p organizes Bni1p into
clusters that nucleate actin assembly during mating
projection formation; some functional redundancy with
BNR1"
/codon_start=1
/product="formin BNI1"
/protein_id="NP_014128.2"
/db_xref="GeneID:855450"
/db_xref="SGD:S000005215"
/translation="MLKNSGSKHSNSKESHSNSSSGIFQNLKRLANSNATNSNTGSPT
YASQQQHSPVGNEVSTSPASSSSFRKLNAPSRSTSTEARPLNKKSTLNTQNLSQYMNG
KLSGDVPVSSQHARSHSMQSKYSYSKRNSSQASNKLTRQHTGQSHSASSLLSQGSLTN
LSKFTTPDGKIYLEMPSDPYEVEVLFEDIMYKRNIFQSLSEDKQEALMGYSIEKKWLI
VKQDLQNELKKMRANTTSSSTASRTSMASDHHPILTANSSLSSPKSVLMTSASSPTST
VYSNSLNHSTTLSSVGTSTSKGKKLVSGSLKKQPSLNNIYRGGAENNTSASTLPGDRT
NRPPIHYVQRILADKLTSDEMKDLWVTLRTEQLDWVDAFIDHQGHIAMANVLMNSIYK
TAPRENLTKELLEKENSFFKCFRVLSMLSQGLYEFSTHRLMTDTVAEGLFSTKLATRK
MATEIFVCMLEKKNKSRFEAVLTSLDKKFRIGQNLHMIQNFKKMPQYFSHLTLESHLK
IIQAWLFAVEQTLDGRGKMGSLVGASDEFKNGGGENAILEYCQWTMVFINHLCSCSDN
INQRMLLRTKLENCGILRIMNKIKLLDYDKVIDQIELYDNNKLDDFNVKLEANNKAFN
VDLHDPLSLLKNLWDICKGTENEKLLVSLVQHLFLSSSKLIEENQNSSKLTKQLKLMD
SLVTNVSVASTSDEETNMNMAIQRLYDAMQTDEVARRAILESRALTKKLEEIQAERDS
LSEKLSKAEHGLVGQLEDELHERDRILAKNQRVMQQLEAELEELKKKHLLEKHQQEVE
LRKMLTILNSRPEESFNKNEGTRGMNSSLNSSEKANIQKVLQDGLSRAKKDYKDDSKK
FGMTLQPNKRLKMLRMQMENIENEARQLEMTNFAEFEKDRLEPPIHIKKPKVKKMKNK
DRKPLVKPQEADVNKLNDLRRALAEIQMESNDISKFNVEERVNELFNEKKSLALKRLK
ELETKYKGFGIDFNVDEIMDSPKKNTGDVETEEDANYASLDPKTYQKKLDEINRITDQ
LLDIQTQTEHEIQVEEDGESDLSSSSSDDESEEIYQDASPTQELRSEHSELSSGSGPG
SFLDALSQKYGTGQNVTASAAFGENNNGSGIGPLHSKVEKTFMNRLRKSTVSSAPYLE
ELTQKVNKVEPYEQNEDEGLDKKSLPENSTASAASAFDKAEKDMRQHVENGKQGRVVN
HEEDKTADFSAVSKLNNTDGAEDLSTQSSVLSSQPPPPPPPPPPVPAKLFGESLEKEK
KSEDDTVKQETTGDSPAPPPPPPPPPPPPMALFGKPKGETPPPPPLPSVLSSSTDGVI
PPAPPMMPASQIKSAVTSPLLPQSPSLFEKYPRPHKKLKQLHWEKLDCTDNSIWGTGK
AEKFADDLYEKGVLADLEKAFAAREIKSLASKRKEDLQKITFLSRDISQQFGINLHMY
SSLSVADLVKKILNCDRDFLQTPSVVEFLSKSEIIEVSVNLARNYAPYSTDWEGVRNL
EDAKPPEKDPNDLQRADQIYLQLMVNLESYWGSRMRALTVVTSYEREYNELLAKLRKV
DKAVSALQESDNLRNVFNVILAVGNFMNDTSKQAQGFKLSTLQRLTFIKDTTNSMTFL
NYVEKIVRLNYPSFNDFLSELEPVLDVVKVSIEQLVNDCKDFSQSIVNVERSVEIGNL
SDSSKFHPLDKVLIKTLPVLPEARKKGDLLEDEVKLTIMEFESLMHTYGEDSGDKFAK
ISFFKKFADFINEYKKAQAQNLAAEEEERLYIKHKKIVEEQQKRAQEKEKQKENSNSP
SSEGNEEDEAEDRRAVMDKLLEQLKNAGPAKSDPSSARKRALVRKKYLSEKDNAPQLL
NDLDTEEGSILYSPEAMDPTADTVIHAESPTPLATRGVMNTSEDLPSPSKTSALEDQE
EISDRARMLLKELRGSDTPVKQNSILDEHLEKLRARKERSIGEASTGNRLSFK"
gene complement(<135940..>137661)
/gene="ALP1"
/locus_tag="YNL270C"
/gene_synonym="APL1"
/db_xref="GeneID:855451"
mRNA complement(<135940..>137661)
/gene="ALP1"
/locus_tag="YNL270C"
/gene_synonym="APL1"
/product="arginine permease ALP1"
/transcript_id="NM_001183108.1"
/db_xref="GeneID:855451"
CDS complement(135940..137661)
/gene="ALP1"
/locus_tag="YNL270C"
/gene_synonym="APL1"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0015174 basic amino
acid transmembrane transporter activity [PMID:10654085]"
/experiment="EXISTENCE:direct assay:GO:0015802 basic amino
acid transport [PMID:10654085]"
/note="Arginine transporter; expression is normally very
low and it is unclear what conditions would induce
significant expression; ALP1 has a paralog, CAN1, that
arose from the whole genome duplication"
/codon_start=1
/product="arginine permease ALP1"
/protein_id="NP_014129.1"
/db_xref="GeneID:855451"
/db_xref="SGD:S000005214"
/translation="MDETVNIQMSKEGQYEINSSSIIKEEEFVDEQYSGENVTKAITT
ERKVEDDAAKETESSPQERREVKRKLKQRHIGMIALGGTIGTGLIIGIGPPLAHAGPV
GALISYLFMGTVIYSVTQSLGEMVTFIPVTSSFSVFAQRFLSPALGATNGYMYWLSWC
FTFALELSVLGKVIQYWTEAVPLAAWIVIFWCLLTSMNMFPVKYYGEFEFCIASIKVI
ALLGFIIFSFCVVCGAGQSDGPIGFRYWRNPGAWGPGIISSDKNEGRFLGWVSSLINA
AFTYQGTELVGITAGEAANPRKALPRAIKKVVVRILVFYILSLFFIGLLVPYNDPKLD
SDGIFVSSSPFMISIENSGTKVLPDIFNAVVLITILSAGNSNVYIGSRVLYSLSKNSL
APRFLSNVTRGGVPYFSVLSTSVFGFLAFLEVSAGSGKAFNWLLNITGVAGFFAWLLI
SFSHIRFMQAIRKRGISRDDLPYKAQMMPFLAYYASFFIALIVLIQGFTAFAPTFQPI
DFVAAYISIFLFLAIWLSFQVWFKCRLLWKLQDIDIDSDRRQIEELVWIEPECKTRWQ
RVWDVLS"
gene <137699..>138094
/gene="BSC4"
/locus_tag="YNL269W"
/db_xref="GeneID:855452"
mRNA <137699..>138094
/gene="BSC4"
/locus_tag="YNL269W"
/product="Bsc4p"
/transcript_id="NM_001183107.1"
/db_xref="GeneID:855452"
CDS 137699..138094
/gene="BSC4"
/locus_tag="YNL269W"
/note="hypothetical protein; protein-coding gene that
evolved de novo via a series of point mutations in
noncoding sequence; ORF exhibits genomic organization
compatible with a translational readthrough-dependent mode
of expression; readthrough is increased upon depletion of
Sup35p; may be involved in DNA repair pathway during
stationary phase and contribute to robustness of cells
when shifted to a nutrient-poor environment"
/codon_start=1
/product="Bsc4p"
/protein_id="NP_014130.1"
/db_xref="GeneID:855452"
/db_xref="SGD:S000005213"
/translation="MSIVLRKSNKKNKNCITSKFYTIHIIKISTPVFRAPIAIGESPY
VEWSCLQVVFRKDMVTKKTTFAQLITRLNHFLCQALKRRDSKTYILCRTAVFGAMTPF
SPRKSHINNKLPMQPRKKKIVIIYVVRFH"
gene <138550..>140385
/gene="LYP1"
/locus_tag="YNL268W"
/db_xref="GeneID:855453"
mRNA <138550..>140385
/gene="LYP1"
/locus_tag="YNL268W"
/product="lysine permease"
/transcript_id="NM_001183106.1"
/db_xref="GeneID:855453"
CDS 138550..140385
/gene="LYP1"
/locus_tag="YNL268W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:40247776]"
/experiment="EXISTENCE:direct assay:GO:0015174 basic amino
acid transmembrane transporter activity [PMID:10654085]"
/experiment="EXISTENCE:direct assay:GO:0015802 basic amino
acid transport [PMID:10654085]"
/experiment="EXISTENCE:direct assay:GO:0032126 eisosome
[PMID:29402931]"
/experiment="EXISTENCE:genetic interaction:GO:0089718
amino acid import across plasma membrane [PMID:40247776]"
/note="Lysine permease; one of three amino acid permeases
(Alp1p, Can1p, Lyp1p) responsible for uptake of cationic
amino acids; broad substrate specificity when
overexpressed in a strain severely deficient for the
uptake of amino acids; predominantly located in the plasma
membrane during exponential growth"
/codon_start=1
/product="lysine permease"
/protein_id="NP_014131.1"
/db_xref="GeneID:855453"
/db_xref="SGD:S000005212"
/translation="MGRFSNIITSNKWDEKQNNIGEQSMQELPEDQIEHEMEAIDPSN
KTTPYSIDEKQYNTKKKHGSLQGGAIADVNSITNSLTRLQVVSHETDINEDEEEAHYE
DKHVKRALKQRHIGMIALGGTIGTGLFVGISTPLSNAGPVGSLIAYIFMGTIVYFVTQ
SLGEMATFIPVTSSITVFSKRFLSPAFGVSNGYMYWFNWAITYAVEVSVIGQVIEYWT
DKVPLAAWIAIFWVIITLMNFFPVKVYGEFEFWVASVKVLAIMGYLIYALIIVCGGSH
QGPIGFRYWRNPGAWGPGIISSDKSEGRFLGWVSSLINAAFTYQGTELVGITAGEAAN
PRKTVPRAINKVVFRIVLFYIMSLFFIGLLVPYNDSRLSASSAVIASSPFVISIQNAG
TYALPDIFNAVVLITVVSAANSNVYVGSRVLYSLARTGNAPKQFGYVTRQGVPYLGVV
CTAALGLLAFLVVNNNANTAFNWLINISTLAGLCAWLFISLAHIRFMQALKHRGISRD
DLPFKAKLMPYGAYYAAFFVTVIIFIQGFQAFCPFKVSEFFTSYISLILLAVVFIGCQ
IYYKCRFIWKLEDIDIDSDRREIEAIIWEDDEPKNLWEKFWAAVA"
gene <140878..>144078
/gene="PIK1"
/locus_tag="YNL267W"
/gene_synonym="PIK120; PIK41"
/db_xref="GeneID:855454"
mRNA <140878..>144078
/gene="PIK1"
/locus_tag="YNL267W"
/gene_synonym="PIK120; PIK41"
/product="1-phosphatidylinositol 4-kinase"
/transcript_id="NM_001183105.1"
/db_xref="GeneID:855454"
CDS 140878..144078
/gene="PIK1"
/locus_tag="YNL267W"
/gene_synonym="PIK120; PIK41"
/EC_number="2.7.1.67"
/experiment="EXISTENCE:direct assay:GO:0004430
1-phosphatidylinositol 4-kinase activity
[PMID:8248783|PMID:8194527]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:8194527|PMID:10587649|PMID:16365163]"
/experiment="EXISTENCE:direct assay:GO:0005802 trans-Golgi
network [PMID:10587649|PMID:16365163]"
/experiment="EXISTENCE:direct assay:GO:0046854
phosphatidylinositol phosphate biosynthetic process
[PMID:8194527|PMID:8248783]"
/experiment="EXISTENCE:mutant phenotype:GO:0004430
1-phosphatidylinositol 4-kinase activity [PMID:10587649]"
/experiment="EXISTENCE:mutant phenotype:GO:0006995
cellular response to nitrogen starvation [PMID:32726584]"
/experiment="EXISTENCE:mutant phenotype:GO:0046854
phosphatidylinositol phosphate biosynthetic process
[PMID:10587649]"
/experiment="EXISTENCE:mutant phenotype:GO:0140504
microlipophagy [PMID:32726584]"
/experiment="EXISTENCE:mutant phenotype:GO:2000786
positive regulation of autophagosome assembly
[PMID:31125705]"
/note="Phosphatidylinositol 4-kinase; catalyzes the first
step in the biosynthesis of
phosphatidylinositol-4,5-biphosphate; may control
cytokinesis through the actin cytoskeleton; required for
autophagosome formation during autophagy and for lipophagy
in both stationary phase cells and during nitrogen
starvation; may control nonselective autophagy and
mitophagy through trafficking of Atg9p; localizes to the
nucleus and to the trans-Golgi network"
/codon_start=1
/product="1-phosphatidylinositol 4-kinase"
/protein_id="NP_014132.1"
/db_xref="GeneID:855454"
/db_xref="SGD:S000005211"
/translation="MHKASSSKKSFDDTIELKKNEQLLKLINSSEFTLHNCVELLCKH
SENIGIHYYLCQKLATFPHSELQFYIPQLVQVLVTMETESMALEDLLLRLRAENPHFA
LLTFWQLQALLTDLSTDPASYGFQVARRVLNNLQTNLFNTSSGSDKNVKIHENVAPAL
VLSSMIMSAIAFPQLSEVTKPLVESQGRRQKAFVFKLARSAMKDFTKNMTLKNTLLNK
KTSRSKRVSSNRSSTPTSPIDLIDPIKTKEDASFRKSRHSEVKLDFDIVDDIGNQVFE
ERISSSIKLPKRKPKYLDNSYVHRTYDGKNINRDGSISNTAKALDGNKGDYISPKGRN
DENNEIGNNEDETGGETEEDADALNSDHFTSSMPDLHNIQPRTSSASSASLEGTPKLN
RTNSQPLSRQAFKNSKKANSSLSQEIDLSQLSTTSKIKMLKANYFRCETQFAIALETI
SQRLARVPTEARLSALRAELFLLNRDLPAEVDIPTLLPPNKKGKLHKLVTITANEAQV
LNSAEKVPYLLLIEYLRDEFDFDPTSETNERLLKKISGNQGGLIFDLNYMNRKENNEN
RNESTLTSNNTRSSVYDSNSFNNGASRNEGLSSTSRSDSASTAHVRTEVNKEEDLGDM
SMVKVRNRTDDEAYRNALVIQSAANVPILPDDSQDRSPELNFGSNLDEVLIENGINSK
NIHSQTDALADQMRVSAVMLAQLDKSPQQLSESTKQIRAQIISSMKEVQDKFGYHDLE
ALHGMAGERKLENDLMTGGIDTSYLGEDWATKKERIRKTSEYGHFENWDLCSVIAKTG
DDLRQEAFAYQMIQAMANIWVKEKVDVWVKRMKILITSANTGLVETITNAMSVHSIKK
ALTKKMIEDAELDDKGGIASLNDHFLRAFGNPNGFKYRRAQDNFASSLAAYSVICYLL
QVKDRHNGNIMIDNEGHVSHIDFGFMLSNSPGSVGFEAAPFKLTYEYIELLGGVEGEA
FKKFVELTKSSFKALRKYADQIVSMCEIMQKDNMQPCFDAGEQTSVQLRQRFQLDLSE
KEVDDFVENFLIGKSLGSIYTRIYDQFQLITQGIYS"
gene complement(<144280..>145281)
/gene="IST1"
/locus_tag="YNL265C"
/db_xref="GeneID:855456"
mRNA complement(join(<144280..145149,145255..>145281))
/gene="IST1"
/locus_tag="YNL265C"
/product="Ist1p"
/transcript_id="NM_001183103.1"
/db_xref="GeneID:855456"
CDS complement(join(144280..145149,145255..145281))
/gene="IST1"
/locus_tag="YNL265C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:18032584]"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:36125415|PMID:18032584|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0042030 ATPase
inhibitor activity [PMID:18032582]"
/experiment="EXISTENCE:genetic interaction:GO:0032511 late
endosome to vacuole transport via multivesicular body
sorting pathway [PMID:18032584]"
/experiment="EXISTENCE:mutant phenotype:GO:0032511 late
endosome to vacuole transport via multivesicular body
sorting pathway [PMID:18032582]"
/experiment="EXISTENCE:mutant phenotype:GO:0042030 ATPase
inhibitor activity [PMID:36125415]"
/experiment="EXISTENCE:mutant phenotype:GO:0099638
endosome to plasma membrane protein transport
[PMID:36125415]"
/note="Protein involved in recycling of membrane
transporters from endosome to plasma membrane; plays a
role in the multivesicular body sorting pathway; functions
and forms a complex with Did2p; recruitment to endosomes
is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III;
also interacts with Vps4p"
/codon_start=1
/product="Ist1p"
/protein_id="NP_014134.1"
/db_xref="GeneID:855456"
/db_xref="SGD:S000005209"
/translation="MAPSMIPFTIKLKTCLKMCIQRLRYAQEKQQAIAKQSRRQVAQL
LLTNKEQKAHYRVETLIHDDIHIELLEILELYCELLLARVQVINDISTEEQLVKEHMD
DGINEAIRSLIYAILFVDEVKELSQLKDLMAWKINVEFVNGVIADHIDVPEKIIKKCS
PSVPKEELVDLYLKEIAKTYDVPYSKLENSLSSSSSNISSDFSDPSGDIEDNDEEKPI
LALDNDDNDNADAKHPITVKKPRQNSENIKNELKIPKDIKKEVIEKKQSEKKTTKRKT
KKEQENDELDELKKRFDALRRK"
gene complement(<145563..>146615)
/gene="PDR17"
/locus_tag="YNL264C"
/gene_synonym="ISS1; SFH4"
/db_xref="GeneID:855457"
mRNA complement(<145563..>146615)
/gene="PDR17"
/locus_tag="YNL264C"
/gene_synonym="ISS1; SFH4"
/product="phosphatidylinositol transporter"
/transcript_id="NM_001183102.1"
/db_xref="GeneID:855457"
CDS complement(145563..146615)
/gene="PDR17"
/locus_tag="YNL264C"
/gene_synonym="ISS1; SFH4"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:20016005]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:12869188|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0008526
phosphatidylinositol transfer activity
[PMID:10848624|PMID:10799527]"
/experiment="EXISTENCE:direct assay:GO:0015914
phospholipid transport [PMID:10848624|PMID:10799527]"
/experiment="EXISTENCE:direct assay:GO:0016126 sterol
biosynthetic process [PMID:36482167]"
/experiment="EXISTENCE:direct assay:GO:0032934 sterol
binding [PMID:36482167]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:12869188]"
/experiment="EXISTENCE:genetic interaction:GO:0008526
phosphatidylinositol transfer activity [PMID:9890948]"
/experiment="EXISTENCE:genetic interaction:GO:0008654
phospholipid biosynthetic process [PMID:9890948]"
/experiment="EXISTENCE:genetic interaction:GO:0009410
response to xenobiotic stimulus [PMID:9890948]"
/experiment="EXISTENCE:genetic interaction:GO:0015914
phospholipid transport [PMID:9890948]"
/experiment="EXISTENCE:genetic interaction:GO:0043001
Golgi to plasma membrane protein transport
[PMID:10848624]"
/experiment="EXISTENCE:genetic interaction:GO:1901352
negative regulation of phosphatidylglycerol biosynthetic
process [PMID:12869188]"
/experiment="EXISTENCE:genetic interaction:GO:2001247
positive regulation of phosphatidylcholine biosynthetic
process [PMID:12869188]"
/experiment="EXISTENCE:mutant phenotype:GO:0006658
phosphatidylserine metabolic process [PMID:10799527]"
/experiment="EXISTENCE:mutant phenotype:GO:0008526
phosphatidylinositol transfer activity [PMID:9890948]"
/experiment="EXISTENCE:mutant phenotype:GO:0015914
phospholipid transport [PMID:9890948]"
/note="Phosphatidylinositol transfer protein (PITP);
downregulates Plb1p-mediated turnover of
phosphatidylcholine; forms a complex with Psd2p which
appears essential for maintenance of vacuolar PE levels;
found in the cytosol and microsomes; homologous to Pdr16p;
deletion affects phospholipid composition"
/codon_start=1
/product="phosphatidylinositol transporter"
/protein_id="NP_014135.1"
/db_xref="GeneID:855457"
/db_xref="SGD:S000005208"
/translation="MGLFSRKRDHTPAVPKEKLIPCDKIFLDPPAKYGNAPLLEPISE
DQNEKYRAVLRHFQDDDLKLPENLNDLDNGTHANDRPLSDWEKFWLSRECFLRYLRAN
KWNTANAIKGLTKTLVWRREIGLTHGKEDKDPLTADKVAVENETGKQVILGFDNAKRP
LYYMKNGRQNTESSFRQVQELVYMMETATTVAPQGVEKITVLVDFKSYKEPGIITDKA
PPISIARMCLNVMQDHYPERLAKCVLINIPWFAWAFLKMMYPFLDPATKAKAIFDEPF
ENHIEPSQLDALYNGLLDFKYKHEVYWPDMVKKVDDLRLKRFDRFLKFGGIVGLSEYD
TKGQHDELKYPVDMVI"
gene complement(<146896..>147840)
/gene="YIF1"
/locus_tag="YNL263C"
/db_xref="GeneID:855458"
mRNA complement(<146896..>147840)
/gene="YIF1"
/locus_tag="YNL263C"
/product="protein transporter YIF1"
/transcript_id="NM_001183101.1"
/db_xref="GeneID:855458"
CDS complement(146896..147840)
/gene="YIF1"
/locus_tag="YNL263C"
/experiment="EXISTENCE:direct assay:GO:0000139 Golgi
membrane [PMID:10970842]"
/experiment="EXISTENCE:direct assay:GO:0030134
COPII-coated ER to Golgi transport vesicle
[PMID:11157978]"
/experiment="EXISTENCE:mutant phenotype:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:10970842]"
/experiment="EXISTENCE:physical interaction:GO:0005789
endoplasmic reticulum membrane [PMID:15659647]"
/note="Integral membrane protein; required for the fusion
of ER-derived COPII transport vesicles with the Golgi;
interacts with Yip1p and Yos1p; localizes to the Golgi,
the ER, and COPII vesicles; homolog of human YIPF3"
/codon_start=1
/product="protein transporter YIF1"
/protein_id="NP_014136.1"
/db_xref="GeneID:855458"
/db_xref="SGD:S000005207"
/translation="MSYNPYAYATSEQNGVNDRFSHTPQQQRPMQIPRNTPVNGQGNA
NMNANVNGSGGGFPFQDPRGSMAFQLGQSAFSNFIGQDNFNQFQETVNKATANAAGSQ
QISTYFQVSTRYVINKLKLILVPFLNGTKNWQRIMDSGNFLPPRDDVNSPDMYMPIMG
LVTYILIWNTQQGLKGSFNPEDLYYKLSSTLAFVCLDLLILKLGLYLLIDSKIPSFSL
VELLCYVGYKFVPLILAQLLTNVTMPFNLNILIKFYLFIAFGVFLLRSVKFNLLSRSG
AEDDDIHVSISKSTVKKCNYFLFVYGFIWQNVLMWLMG"
gene <148212..>154880
/gene="POL2"
/locus_tag="YNL262W"
/gene_synonym="DUN2"
/db_xref="GeneID:855459"
mRNA <148212..>154880
/gene="POL2"
/locus_tag="YNL262W"
/gene_synonym="DUN2"
/product="DNA polymerase epsilon catalytic subunit"
/transcript_id="NM_001183100.1"
/db_xref="GeneID:855459"
CDS 148212..154880
/gene="POL2"
/locus_tag="YNL262W"
/gene_synonym="DUN2"
/EC_number="2.7.7.7"
/experiment="EXISTENCE:direct assay:GO:0003690
double-stranded DNA binding [PMID:14622139|PMID:16916794]"
/experiment="EXISTENCE:direct assay:GO:0003697
single-stranded DNA binding [PMID:14622139]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0003887
DNA-directed DNA polymerase activity [PMID:16369485]"
/experiment="EXISTENCE:direct assay:GO:0005657 replication
fork [PMID:15773893|PMID:18851837]"
/experiment="EXISTENCE:direct assay:GO:0006261
DNA-templated DNA replication
[PMID:16369485|PMID:14622139]"
/experiment="EXISTENCE:direct assay:GO:0008622 epsilon DNA
polymerase complex [PMID:2406268|PMID:12571237]"
/experiment="EXISTENCE:direct assay:GO:0032183 SUMO
binding [PMID:31765407]"
/experiment="EXISTENCE:direct assay:GO:0042276 error-prone
translesion synthesis [PMID:19776424]"
/experiment="EXISTENCE:genetic interaction:GO:0006303
double-strand break repair via nonhomologous end joining
[PMID:18437220]"
/experiment="EXISTENCE:genetic interaction:GO:0031573
mitotic intra-S DNA damage checkpoint signaling
[PMID:18851837|PMID:8895664]"
/experiment="EXISTENCE:mutant phenotype:GO:0003887
DNA-directed DNA polymerase activity [PMID:1537345]"
/experiment="EXISTENCE:mutant phenotype:GO:0006272 leading
strand elongation [PMID:17615360]"
/experiment="EXISTENCE:mutant phenotype:GO:0006284
base-excision repair [PMID:8423775]"
/experiment="EXISTENCE:mutant phenotype:GO:0006297
nucleotide-excision repair, DNA gap filling
[PMID:11452038]"
/experiment="EXISTENCE:mutant phenotype:GO:0006302
double-strand break repair [PMID:10025407]"
/experiment="EXISTENCE:mutant phenotype:GO:0006303
double-strand break repair via nonhomologous end joining
[PMID:18437220]"
/experiment="EXISTENCE:mutant phenotype:GO:0007064 mitotic
sister chromatid cohesion [PMID:12665575]"
/experiment="EXISTENCE:mutant phenotype:GO:0008310
single-stranded DNA 3'-5' DNA exonuclease activity
[PMID:1658784]"
/experiment="EXISTENCE:mutant phenotype:GO:0031573 mitotic
intra-S DNA damage checkpoint signaling [PMID:8895664]"
/experiment="EXISTENCE:mutant phenotype:GO:0033314 mitotic
DNA replication checkpoint signaling [PMID:9792727]"
/experiment="EXISTENCE:mutant phenotype:GO:0035822 gene
conversion [PMID:20595613]"
/experiment="EXISTENCE:mutant phenotype:GO:0045004 DNA
replication proofreading [PMID:21124948|PMID:1658784]"
/experiment="EXISTENCE:physical interaction:GO:0031573
mitotic intra-S DNA damage checkpoint signaling
[PMID:18851837]"
/note="Catalytic subunit of DNA polymerase (II) epsilon; a
chromosomal DNA replication polymerase that exhibits
processivity and proofreading exonuclease activity;
participates in leading-strand synthesis during DNA
replication; also involved in DNA synthesis during DNA
repair; interacts extensively with Mrc1p"
/codon_start=1
/product="DNA polymerase epsilon catalytic subunit"
/protein_id="NP_014137.1"
/db_xref="GeneID:855459"
/db_xref="SGD:S000005206"
/translation="MMFGKKKNNGGSSTARYSAGNKYNTLSNNYALSAQQLLNASKID
DIDSMMGFERYVPPQYNGRFDAKDIDQIPGRVGWLTNMHATLVSQETLSSGSNGGGNS
NDGERVTTNQGISGVDFYFLDEEGGSFKSTVVYDPYFFIACNDESRVNDVEELVKKYL
ESCLKSLQIIRKEDLTMDNHLLGLQKTLIKLSFVNSNQLFEARKLLRPILQDNANNNV
QRNIYNVAANGSEKVDAKHLIEDIREYDVPYHVRVSIDKDIRVGKWYKVTQQGFIEDT
RKIAFADPVVMAFDIETTKPPLKFPDSAVDQIMMISYMIDGEGFLITNREIISEDIED
FEYTPKPEYPGFFTIFNENDEVALLQRFFEHIRDVRPTVISTFNGDFFDWPFIHNRSK
IHGLDMFDEIGFAPDAEGEYKSSYCSHMDCFRWVKRDSYLPQGSQGLKAVTQSKLGYN
PIELDPELMTPYAFEKPQHLSEYSVSDAVATYYLYMKYVHPFIFSLCTIIPLNPDETL
RKGTGTLCEMLLMVQAYQHNILLPNKHTDPIERFYDGHLLESETYVGGHVESLEAGVF
RSDLKNEFKIDPSAIDELLQELPEALKFSVEVENKSSVDKVTNFEEIKNQITQKLLEL
KENNIRNELPLIYHVDVASMYPNIMTTNRLQPDSIKAERDCASCDFNRPGKTCARKLK
WAWRGEFFPSKMDEYNMIKRALQNETFPNKNKFSKKKVLTFDELSYADQVIHIKKRLT
EYSRKVYHRVKVSEIVEREAIVCQRENPFYVDTVKSFRDRRYEFKGLAKTWKGNLSKI
DPSDKHARDEAKKMIVLYDSLQLAHKVILNSFYGYVMRKGSRWYSMEMAGITCLTGAT
IIQMARALVERVGRPLELDTDGIWCILPKSFPETYFFTLENGKKLYLSYPCSMLNYRV
HQKFTNHQYQELKDPLNYIYETHSENTIFFEVDGPYKAMILPSSKEEGKGIKKRYAVF
NEDGSLAELKGFELKRRGELQLIKNFQSDIFKVFLEGDTLEGCYSAVASVCNRWLDVL
DSHGLMLEDEDLVSLICENRSMSKTLKEYEGQKSTSITTARRLGDFLGEDMVKDKGLQ
CKYIISSKPFNAPVTERAIPVAIFSADIPIKRSFLRRWTLDPSLEDLDIRTIIDWGYY
RERLGSAIQKIITIPAALQGVSNPVPRVEHPDWLKRKIATKEDKFKQTSLTKFFSKTK
NVPTMGKIKDIEDLFEPTVEEDNAKIKIARTTKKKAVSKRKRNQLTNEEDPLVLPSEI
PSMDEDYVGWLNYQKIKWKIQARDRKRRDQLFGNTNSSRERSALGSMIRKQAESYANS
TWEVLQYKDSGEPGVLEVFVTINGKVQNITFHIPKTIYMKFKSQTMPLQKIKNCLIEK
SSASLPNNPKTSNPAGGQLFKITLPESVFLEEKENCTSIFNDENVLGVFEGTITPHQR
AIMDLGASVTFRSKAMGALGKGIQQGFEMKDLSMAENERYLSGFSMDIGYLLHFPTSI
GYEFFSLFKSWGDTITILVLKPSNQAQEINASSLGQIYKQMFEKKKGKIETYSYLVDI
KEDINFEFVYFTDISKLYRRLSQETTKLKEERGLQFLLLLQSPFITKLLGTIRLLNQM
PIVKLSLNEVLLPQLNWQPTLLKKLVNHVLSSGSWISHLIKLSQYSNIPICNLRLDSM
DYIIDVLYARKLKKENIVLWWNEKAPLPDHGGIQNDFDLNTSWIMNDSEFPKINNSGV
YDNVVLDVGVDNLTVNTILTSALINDAEGSDLVNNNMGIDDKDAVINSPSEFVHDAFS
NDALNVLRGMLKEWWDEALKENSTADLLVNSLASWVQNPNAKLFDGLLRYHVHNLTKK
ALLQLVNEFSALGSTIVYADRNQILIKTNKYSPENCYAYSQYMMKAVRTNPMFSYLDL
NIKRYWDLLIWMDKFNFSGLACIEIEEKENQDYTAVSQWQLKKFLSPIYQPEFEDWMM
IILDSMLKTKQSYLKLNSGTQRPTQIVNVKKQDKEDSVENSLNGFSHLFSKPLMKRVK
KLFKNQQEFILDPQYEADYVIPVLPGSHLNVKNPLLELVKSLCHVMLLSKSTILEIRT
LRKELLKIFELREFAKVAEFKDPSLSLVVPDFLCEYCFFISDIDFCKAAPESIFSCVR
CHKAFNQVLLQEHLIQKLRSDIESYLIQDLRCSRCHKVKRDYMSAHCPCAGAWEGTLP
RESIVQKLNVFKQVAKYYGFDILLSCIADLTI"
gene <155100..>156539
/gene="ORC5"
/locus_tag="YNL261W"
/db_xref="GeneID:855460"
mRNA <155100..>156539
/gene="ORC5"
/locus_tag="YNL261W"
/product="origin recognition complex subunit 5"
/transcript_id="NM_001183099.1"
/db_xref="GeneID:855460"
CDS 155100..156539
/gene="ORC5"
/locus_tag="YNL261W"
/experiment="EXISTENCE:direct assay:GO:0003688 DNA
replication origin binding [PMID:16824194]"
/experiment="EXISTENCE:direct assay:GO:0005656 nuclear
pre-replicative complex [PMID:16824194|PMID:9335335]"
/experiment="EXISTENCE:direct assay:GO:0005664 nuclear
origin of replication recognition complex [PMID:9372948]"
/experiment="EXISTENCE:direct assay:GO:0006267
pre-replicative complex assembly involved in nuclear cell
cycle DNA replication [PMID:16824194]"
/experiment="EXISTENCE:direct assay:GO:0030466 silent
mating-type cassette heterochromatin formation
[PMID:12897051]"
/experiment="EXISTENCE:direct assay:GO:0031261 DNA
replication preinitiation complex [PMID:9554851]"
/experiment="EXISTENCE:genetic interaction:GO:0006270 DNA
replication initiation [PMID:7579692]"
/experiment="EXISTENCE:mutant phenotype:GO:0005524 ATP
binding [PMID:9038340]"
/experiment="EXISTENCE:mutant phenotype:GO:0005664 nuclear
origin of replication recognition complex [PMID:9372948]"
/experiment="EXISTENCE:mutant phenotype:GO:0006270 DNA
replication initiation [PMID:7579692|PMID:16716188]"
/note="Subunit of the origin recognition complex (ORC);
ORC directs DNA replication by binding to replication
origins and is also involved in transcriptional silencing"
/codon_start=1
/product="origin recognition complex subunit 5"
/protein_id="NP_014138.1"
/db_xref="GeneID:855460"
/db_xref="SGD:S000005205"
/translation="MNVTTPEVAFREYQTNCLASYISADPDITPSNLILQGYSGTGKT
YTLKKYFNANPNLHAVWLEPVELVSWKPLLQAIARTVQYKLKTLYPNIPTTDYDPLQV
EEPFLLVKTLHNIFVQYESLQEKTCLFLILDGFDSLQDLDAALFNKYIKLNELLPKDS
KINIKFIYTMLETSFLQRYSTHCIPTVMFPRYNVDEVSTILVMSRCGELMEDSCLRKR
IIEEQITDCTDDQFQNVAANFIHLIVQAFHSYTGNDIFALNDLIDFKWPKYVSRITKE
NIFEPLALYKSAIKLFLSTDDNLSENGQGESAITTNRDDLENSQTYDLSIISKYLLIA
SYICSYLEPRYDASIFSRKTRIIQGRAAYGRRKKKEVNPRYLQPSLFAIERLLAIFQA
IFPIQGKAESGSLSALREESLMKANIEVFQNLSELHTLKLIATTMNKNIDYLSPKVRW
KVNVPWEIIKEISESVHFNISDYFSDIHE"
gene complement(<156859..>157347)
/gene="LTO1"
/locus_tag="YNL260C"
/db_xref="GeneID:855461"
mRNA complement(<156859..>157347)
/gene="LTO1"
/locus_tag="YNL260C"
/product="ribosome biosynthesis protein LTO1"
/transcript_id="NM_001183098.2"
/db_xref="GeneID:855461"
CDS complement(156859..157347)
/gene="LTO1"
/locus_tag="YNL260C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:23318452|PMID:14690591]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14690591]"
/experiment="EXISTENCE:direct assay:GO:0030674
protein-macromolecule adaptor activity [PMID:26182403]"
/experiment="EXISTENCE:direct assay:GO:0062092 Yae1-Lto1
complex [PMID:26182403]"
/experiment="EXISTENCE:mutant phenotype:GO:0030674
protein-macromolecule adaptor activity [PMID:26182403]"
/experiment="EXISTENCE:mutant phenotype:GO:0051604 protein
maturation [PMID:26182403]"
/experiment="EXISTENCE:mutant phenotype:GO:0062092
Yae1-Lto1 complex [PMID:26182403]"
/note="Substrate-specific adaptor protein involved in
apo-Rli1p maturation; subunit of the Yae1-Lto1 complex,
recruiting apo-Rli1p to the CIA targeting complex,
facilitating the insertion of an Fe/S cluster by the Fe-S
biosynthetic machinery; deca-GX3 motif crucial for complex
formation; essential for aerobic but not anaerobic growth;
homolog of human ORAOV1, which is overexpressed in solid
tumors; co-expression of ORAOV1 and YAE1 homolog YAE1D1
restores growth and Rli1p maturation after LTO1 depletion"
/codon_start=1
/product="ribosome biosynthesis protein LTO1"
/protein_id="NP_014139.2"
/db_xref="GeneID:855461"
/db_xref="SGD:S000005204"
/translation="MDFDNLLNLEEQYYQEGFLEGQNENIKQSFLEGKQYGLQVGFQR
FTLLGQMEGLCDVIESYGLHSPTLEKNIHTIRTLMKGLKMNNDDESVMEFERVLIKLK
NKFRTILITLHRLVKDKRTPTVTFEVFEDVSRAIAGEIRGFVENEDIAKNKTKQNQAQ
SW"
gene complement(<157644..>157865)
/gene="ATX1"
/locus_tag="YNL259C"
/db_xref="GeneID:855462"
mRNA complement(<157644..>157865)
/gene="ATX1"
/locus_tag="YNL259C"
/product="copper metallochaperone ATX1"
/transcript_id="NM_001183097.1"
/db_xref="GeneID:855462"
CDS complement(157644..157865)
/gene="ATX1"
/locus_tag="YNL259C"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:9083054]"
/experiment="EXISTENCE:direct assay:GO:0016531 copper
chaperone activity [PMID:17961510|PMID:9346482]"
/experiment="EXISTENCE:mutant phenotype:GO:0006825 copper
ion transport [PMID:9083054]"
/experiment="EXISTENCE:mutant phenotype:GO:0006879
intracellular iron ion homeostasis [PMID:9083054]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress [PMID:7731983]"
/note="Cytosolic copper metallochaperone; transports
copper to the secretory vesicle copper transporter Ccc2p
for eventual insertion into Fet3p, which is a multicopper
oxidase required for high-affinity iron uptake; human
homolog ATOX1 can complement yeast atx1 mutant;
overexpression of human ATOX1 suppresses lysine auxotrophy
of the yeast sod1 null mutant, as does overexpression of
yeast ATX1"
/codon_start=1
/product="copper metallochaperone ATX1"
/protein_id="NP_014140.1"
/db_xref="GeneID:855462"
/db_xref="SGD:S000005203"
/translation="MAEIKHYQFNVVMTCSGCSGAVNKVLTKLEPDVSKIDISLEKQL
VDVYTTLPYDFILEKIKKTGKEVRSGKQL"
gene complement(<158109..>160373)
/gene="DSL1"
/locus_tag="YNL258C"
/gene_synonym="RNS1"
/db_xref="GeneID:855463"
mRNA complement(<158109..>160373)
/gene="DSL1"
/locus_tag="YNL258C"
/gene_synonym="RNS1"
/product="Dsl1p"
/transcript_id="NM_001183096.1"
/db_xref="GeneID:855463"
CDS complement(158109..160373)
/gene="DSL1"
/locus_tag="YNL258C"
/gene_synonym="RNS1"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:15942868]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:11739780]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0070939 Dsl1/NZR
complex [PMID:19151722]"
/experiment="EXISTENCE:genetic interaction:GO:0006890
retrograde vesicle-mediated transport, Golgi to
endoplasmic reticulum [PMID:11493604]"
/experiment="EXISTENCE:mutant phenotype:GO:0006890
retrograde vesicle-mediated transport, Golgi to
endoplasmic reticulum [PMID:11493604|PMID:11739780]"
/experiment="EXISTENCE:mutant phenotype:GO:0032581
ER-dependent peroxisome organization [PMID:19346454]"
/experiment="EXISTENCE:physical interaction:GO:0070939
Dsl1/NZR complex [PMID:15958492]"
/note="Peripheral membrane protein needed for Golgi-to-ER
retrograde traffic; mediates Sey1p-independent homotypic
ER fusion; forms Dsl1 tethering complex with Sec39p and
Tip20p that forms a stable complex with ER SNAREs Sec20p,
Ufe1p and Use1p and is functionally conserved from yeast
to mammalian cells; component of the ER target site that
interacts with coatomer; interacts with different subunits
of COPI vesicle coat; interacts with Cin5p; homolog of fly
and human ZW10 gene"
/codon_start=1
/product="Dsl1p"
/protein_id="NP_014141.1"
/db_xref="GeneID:855463"
/db_xref="SGD:S000005202"
/translation="MESLFPNKGEIIRELLKDPLILKNDSKRSNGSELELDSSDLLQR
EAILANELNILDNLKTFLNLIKEVKTNLNILELENCYYSLQSLRKKMRNNAAYLKQSF
NFQQSISTYVDTLHLELVSTLYKILTNGFWKITENSIQFTPTVEWGKDKVHIEYDTFM
DFVAQQYFPKGSLDNQAWFILDMTSADSQEQVRAKLNTIMKEYMNLSRIVSMIKNSIF
ISGKEISYENEKNILVFSKSSSHGQHCVSTVLTSFEAVCDFMLDGLAFRDRKTLSYEL
GPLFNTEFTKFVKNNASIILESLDSPLKNLVSVINNKLTRLVAKSEVTNWTHSGKEIQ
DLLMNKQLYYNLLLDKVLESHISEIRSIFEDPKKSWQNLEVVELTTSNTNTMSEKIGK
NDSDVQNEKELHNAVSKDDDWNWEVEDDDADAWGDEIDVNIDDEEEKTNQEKEKEPEE
EENAWDEAWAIDENIDDASLENGKEHLKAHDVGSLDKDHIEVTQLPKLFLAISQNFKS
SFADSHVDEQYFAYKYNLLQTSYMAMCTANFSHNWCQLYVDMRYLIERDEKLYRIKEL
TRNLLETKLNMKYRIVCQLIRHQLTEFRENERNPSWDATIEKLLPYILKEIVRPLQKI
RGEEGSRYLLSFLNFLYNDCVTKEILKWQIISEVNSENLGELVSLLVNNTDIQLLAKE
PSYKKMREKFATMGKFLPLHLKEIMEMFYNGDFYLFATDELIQWIELLFADTPLRRNA
IDDIYEIRGTALDD"
gene complement(<160631..>164320)
/gene="SIP3"
/locus_tag="YNL257C"
/gene_synonym="LAM3"
/db_xref="GeneID:855464"
mRNA complement(<160631..>164320)
/gene="SIP3"
/locus_tag="YNL257C"
/gene_synonym="LAM3"
/product="Sip3p"
/transcript_id="NM_001183095.1"
/db_xref="GeneID:855464"
CDS complement(160631..164320)
/gene="SIP3"
/locus_tag="YNL257C"
/gene_synonym="LAM3"
/experiment="EXISTENCE:direct assay:GO:0003712
transcription coregulator activity [PMID:8127709]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0032541 cortical
endoplasmic reticulum [PMID:26001273]"
/experiment="EXISTENCE:direct assay:GO:0045944 positive
regulation of transcription by RNA polymerase II
[PMID:8127709]"
/experiment="EXISTENCE:mutant phenotype:GO:0032366
intracellular sterol transport [PMID:26001273]"
/note="Putative sterol transfer protein; has a probable
role in retrograde transport of sterols from the plasma
membrane to the ER; co-localizes to puncta in the cortical
ER with Ysp2p; contains GRAM, StART-like (VASt) and two
PH-like domains; one of 6 StART-like domain-containing
proteins in yeast that may be involved in sterol transfer
between intracellular membranes; conserved across
eukaryotes; previously identified as a transcription
cofactor that interacts with DNA-bound Snf1p"
/codon_start=1
/product="Sip3p"
/protein_id="NP_014142.1"
/db_xref="GeneID:855464"
/db_xref="SGD:S000005201"
/translation="MSVHGRDPKKRQLRLISVAFKEASIDSPSFRASVNFFQTRVDAL
EDWIEKTVDFFDQKYKVSFEDFRRAKETLLSQLLPPPALLSNGFVSNQSFTPRLIDSF
NKDYYDFSMKLLQIVKGDDSSHSTALLELMTTAIEPYRNVRKNFDFYQGKYDSMLASY
QAIRISKTSLEPSSIKSDALQLFEVQKNYLKASLDLISAISAVKLSLDKFILESMKVL
KSRSIFITKDSGRKIDLSPCINEYLDNYAIWVENSIEGSKVLDSDISNAKKQAYRYTL
KRITPSSDTSDYNIRSIHSSKLLSKDTQVPPKSPEKSGWLYMKTQVGKPTREIWVRRW
CFLKNAVFGMFLLSPSKTYVEETDKFGVFLTNVRYDPEEDRKFCFEVKIFGNKVTEAH
DNMSKDITLVFQTSNYLDLKSWLIAFEATKKYVMSIQHDSLEYELAFKRFSPKFFEFA
SSTTTSIDQLITTFDKETESLYETLNCSISEYDILTLGEEKVFQFQMPTTPISTKMTQ
LAILSNFLTKGSWFPNAVLANIWGTTDWSEYTILPGKGKKPSSLLTIDGKRLPIRNST
IYPQYYSNELKVLDLQFKSLVFSPDQRLEKLPEELLLFKFEALWCPNKKQKFSATCFC
TKDYIYCYMNSMEFICLTKISLSEIVSVEADRSSKKTLKLYDASGLQMKAIVLFSDYK
LIASKLQYLLENKAIKNPNSNEEILVKFEQMEKESQEKKQEELYKIEQENSFDRKATS
VSKIIKSRVTFWEMSDDASTLLNRLKKLQTEYSITYNHEYEISSKGLAHILFGDKSNA
FPKCLFLARKDGEEHGKRFWYKNKDINGKSQLVRKIPFRLDMTGNFLNTGKYHRDKES
KMIFATQRIVKIVDNKYYEVDLDPFFVKVPFCHLLKLSIKFVITESYDVDNHLEIKLN
MTASSSSLHVLYKLEYIDSRTGKTIEKLSLAEIICQTWALKFAHSEFLLIRRVLRYYL
EKIGKHGKVIKAIKLCGILGVLSNKSEEPATEKNGNSKESESMQYDIRYSCTILFLVF
IKLMVYRVTNLTFVFFRILIGILLLCAEKFSRINRMMVVGLLASIMINILLSEKASVP
YWSIKRAEKLFHDRLGSDKFTMQRAIYISDSDLLSSQLSVPSNNPIFEKFSEDNFNKD
YQYSETRKQLAMRRNELLIELRILQDMEKQLVHDDYEKFLLEEVNKCSMVSIEMTDLW
FNDTQLQNYCSICNEELEKLRPPIT"
gene <164623..>167097
/gene="FOL1"
/locus_tag="YNL256W"
/db_xref="GeneID:855465"
mRNA <164623..>167097
/gene="FOL1"
/locus_tag="YNL256W"
/product="trifunctional dihydropteroate
synthetase/dihydrohydroxymethylpterin
pyrophosphokinase/dihydroneopterin aldolase FOL1"
/transcript_id="NM_001183094.1"
/db_xref="GeneID:855465"
CDS 164623..167097
/gene="FOL1"
/locus_tag="YNL256W"
/EC_number="2.5.1.15"
/EC_number="2.7.6.3"
/EC_number="4.1.2.25"
/experiment="EXISTENCE:direct assay:GO:0003848
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
diphosphokinase activity [PMID:15169867]"
/experiment="EXISTENCE:direct assay:GO:0004150
dihydroneopterin aldolase activity [PMID:15169867]"
/experiment="EXISTENCE:direct assay:GO:0004156
dihydropteroate synthase activity [PMID:15169867]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:8813764]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005740
mitochondrial envelope [PMID:15169867]"
/experiment="EXISTENCE:mutant phenotype:GO:0004156
dihydropteroate synthase activity [PMID:11731153]"
/experiment="EXISTENCE:mutant phenotype:GO:0046654
tetrahydrofolate biosynthetic process
[PMID:11731153|PMID:15169867]"
/note="Multifunctional enzyme of the folic acid
biosynthesis pathway; has dihydropteroate synthetase,
dihydro-6-hydroxymethylpterin pyrophosphokinase, and
dihydroneopterin aldolase activities"
/codon_start=1
/product="trifunctional dihydropteroate
synthetase/dihydrohydroxymethylpterin
pyrophosphokinase/dihydroneopterin aldolase FOL1"
/protein_id="NP_014143.2"
/db_xref="GeneID:855465"
/db_xref="SGD:S000005200"
/translation="MSKLFSTVNSARHSVPLGGMRDYVHIKKLEMNTVLGPDSWNQLM
PQKCLLSLDMGTDFSKSAATDDLKYSLNYAVISRDLTNFVSKKKNWGSVSNLAKSVSQ
FVMDKYSGVECLNLEVQADTTHIRSDHISCIIQQERGNPESQEFDVVRISELKMLTLI
GVFTFERLKKQYVTLDIKLPWPKKAELPPPVQSIIDNVVKFVEESNFKTVEALVESVS
AVIAHNEYFQKFPDSPLVVKVLKLNAITATEGVGVSCIREPREIAMVNIPYLSSIHES
SDIKFQLSSSQNTPIEGKNTWKRAFLAFGSNIGDRFKHIQMALQLLSREKTVKLRNIS
SIFESEPMYFKDQTPFMNGCVEVETLLTPSELLKLCKKIEYEELQRVKHFDNGPRTID
LDIVMFLNSAGEDIIVNEPDLNIPHPRMLERTFVLEPLCELISPVHLHPVTAEPIVDH
LKQLYDKQHDEDTLWKLVPLPYRSGVEPRFLKFKTATKLDEFTGETNRITVSPTYIMA
IFNATPDSFSDGGEHFADIESQLNDIIKLCKDALYLHESVIIDVGGCSTRPNSIQASE
EEEIRRSIPLIKAIRESTELPQDKVILSIDTYRSNVAKEAIKVGVDIINDISGGLFDS
NMFAVIAENPEICYILSHTRGDISTMNRLAHYENFALGDSIQQEFVHNTDIQQLDDLK
DKTVLIRNVGQEIGERYIKAIDNGVKRWQILIDPGLGFAKTWKQNLQIIRHIPILKNY
SFTMNSNNSQVYVNLRNMPVLLGPSRKKFIGHITKDVDAKQRDFATGAVVASCIGFGS
DMVRVHDVKNCSKSIKLADAIYKGLE"
gene complement(<167329..>167790)
/gene="GIS2"
/locus_tag="YNL255C"
/db_xref="GeneID:855466"
mRNA complement(<167329..>167790)
/gene="GIS2"
/locus_tag="YNL255C"
/product="mRNA-binding translational activator GIS2"
/transcript_id="NM_001183093.1"
/db_xref="GeneID:855466"
CDS complement(167329..167790)
/gene="GIS2"
/locus_tag="YNL255C"
/experiment="EXISTENCE:direct assay:GO:0000932 P-body
[PMID:23222640|PMID:23285195]"
/experiment="EXISTENCE:direct assay:GO:0003697
single-stranded DNA binding [PMID:21232131]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:21232131|PMID:23222640|PMID:20844764]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:23285195]"
/experiment="EXISTENCE:direct assay:GO:0045182 translation
regulator activity [PMID:21277287]"
/experiment="EXISTENCE:direct assay:GO:2000767 positive
regulation of cytoplasmic translation [PMID:21277287]"
/note="Translational activator for mRNAs with internal
ribosome entry sites; associates with polysomes and binds
to a specific subset of mRNAs; localizes to RNA processing
bodies (P bodies) and to stress granules; may have a role
in translation regulation under stress conditions;
ortholog of human ZNF9/CNBP, a gene involved in myotonic
dystrophy type 2"
/codon_start=1
/product="mRNA-binding translational activator GIS2"
/protein_id="NP_014144.1"
/db_xref="GeneID:855466"
/db_xref="SGD:S000005199"
/translation="MSQKACYVCGKIGHLAEDCDSERLCYNCNKPGHVQTDCTMPRTV
EFKQCYNCGETGHVRSECTVQRCFNCNQTGHISRECPEPKKTSRFSKVSCYKCGGPNH
MAKDCMKEDGISGLKCYTCGQAGHMSRDCQNDRLCYNCNETGHISKDCPKA"
gene complement(<168043..>169248)
/gene="RTC4"
/locus_tag="YNL254C"
/db_xref="GeneID:855467"
mRNA complement(<168043..>169248)
/gene="RTC4"
/locus_tag="YNL254C"
/product="Rtc4p"
/transcript_id="NM_001183092.1"
/db_xref="GeneID:855467"
CDS complement(168043..169248)
/gene="RTC4"
/locus_tag="YNL254C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="hypothetical protein; null mutation suppresses
cdc13-1 temperature sensitivity; (GFP)-fusion protein
localizes to both the cytoplasm and the nucleus"
/codon_start=1
/product="Rtc4p"
/protein_id="NP_014145.1"
/db_xref="GeneID:855467"
/db_xref="SGD:S000005198"
/translation="MVGPGLGINRVRRKGVYSTKKGSGDNLLLMKRQGKHDIHDRESD
DLSGHDAFSPSKKRGKIDSITEDEIEVKKLSTVATFDKLSRSFPNSEVQAAKNAALRG
KEKEEEKVVSIPLIQNLKNEDIESIKCRNNNLLDGKKLLLEAELSAVEDNQIFSSSFP
EDKKLSLQSCLSSKEQIIKKLQVREEYMSKFKLPPMLFSDELLTEVEPFMPIVMDILE
GKISSVYYFEAKNAFKNSQKAYLSVDEFRKLNLNKFTAGFYGLKRQLRVGEEIAKRYK
RALTHNQPATLKWWGITDFCNYVLAPETLTSFCIYQLNLSNKSCSSKTPNKHPKQQLN
EKEYYYDPELRMLAYDLLEDTVEYGIIVADSDPIEQWEAAIEEDRLRELKLDVHNYSS
RRWRLDTHD"
rep_origin 169675..169753
/note="ARS1411; ARS activated in late S phase"
/db_xref="SGD:S000077373"
gene <170018..>171286
/gene="TEX1"
/locus_tag="YNL253W"
/db_xref="GeneID:855468"
mRNA <170018..>171286
/gene="TEX1"
/locus_tag="YNL253W"
/product="Tex1p"
/transcript_id="NM_001183091.1"
/db_xref="GeneID:855468"
CDS 170018..171286
/gene="TEX1"
/locus_tag="YNL253W"
/experiment="EXISTENCE:curator inference:GO:0006406 mRNA
export from nucleus [PMID:11979277]"
/experiment="EXISTENCE:direct assay:GO:0000346
transcription export complex [PMID:11979277]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/note="Protein involved in mRNA export; component of the
transcription export (TREX) complex"
/codon_start=1
/product="Tex1p"
/protein_id="NP_014146.1"
/db_xref="GeneID:855468"
/db_xref="SGD:S000005197"
/translation="MSTIGAVDILNQKTITSEVAASVTSKYLQSTFSKGNTSHIEDKR
FIHVSSRSHSRFTSTPITPNEILSLKFHVSGSSMAYSRMDGSLTVWFIKDASFDKSVE
VYIPDCCGSDKLATDLSWNPTSLNQIAVVSNSSEISLLLINEKSLTASKLRTLSLGSK
TKVNTCLYDPLGNWLLAATKSEKIYLFDVKKDHSSVCSLNISDISQEDNDVVYSLAWS
NGGSHIFIGFKSGYLAILKAKHGILEVCTKIKAHTGPITEIKMDPWGRNFITGSIDGN
CYVWNMKSLCCELIINDLNSAVTTLDVCHLGKILGICTEDEMVYFYDLNSGNLLHSKS
LANYKTDPVLKFYPDKSWYIMSGKNDTLSNHFVKNEKNLITYWKDMFDNTMIEKRRKN
NGGGNNHNKRTSKNTDRIGKDRPSRFNSKK"
gene complement(<171441..>172286)
/gene="MRPL17"
/locus_tag="YNL252C"
/gene_synonym="mL46; MRPL30"
/db_xref="GeneID:855469"
mRNA complement(<171441..>172286)
/gene="MRPL17"
/locus_tag="YNL252C"
/gene_synonym="mL46; MRPL30"
/product="mitochondrial 54S ribosomal protein mL46 MRPL17"
/transcript_id="NM_001183090.1"
/db_xref="GeneID:855469"
CDS complement(171441..172286)
/gene="MRPL17"
/locus_tag="YNL252C"
/gene_synonym="mL46; MRPL30"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:12392552|PMID:9151978]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:9151978|PMID:12392552]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005762
mitochondrial large ribosomal subunit
[PMID:12392552|PMID:9151978]"
/note="Mitochondrial ribosomal protein of the large
subunit"
/codon_start=1
/product="mitochondrial 54S ribosomal protein mL46 MRPL17"
/protein_id="NP_014147.1"
/db_xref="GeneID:855469"
/db_xref="SGD:S000005196"
/translation="MKVNLMLKRGLATATATASSAPPKIKVGVLLSRIPIIKSELNEL
EKKYYEYQSELEKRLMWTFPAYFYFKKGTVAEHKFLSLQKGPISKKNGIWFPRGIPDI
KHGRERSTKQEVKLSDDSTVAFSNNQKEQSKDDVNRPVIPNDRITEADRSNDMKSLER
QLSRTLYLLVKDKSGTWKFPNFDLSDESKPLHVHAENELKLLSGDQIYTWSVSATPIG
VLQDERNRTAEFIVKSHILAGKFDLVASKNDAFEDFAWLTKGEISEYVPKDYFNKTEF
LLADN"
gene complement(<172588..>174315)
/gene="NRD1"
/locus_tag="YNL251C"
/db_xref="GeneID:855470"
mRNA complement(<172588..>174315)
/gene="NRD1"
/locus_tag="YNL251C"
/product="Nrd1 complex RNA-binding subunit"
/transcript_id="NM_001183089.1"
/db_xref="GeneID:855470"
CDS complement(172588..174315)
/gene="NRD1"
/locus_tag="YNL251C"
/experiment="EXISTENCE:direct assay:GO:0001068
transcription regulatory region RNA binding
[PMID:21084293]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:9618475]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0019904 protein
domain specific binding [PMID:18660819]"
/experiment="EXISTENCE:direct assay:GO:0030847 termination
of RNA polymerase II transcription, exosome-dependent
[PMID:16427013]"
/experiment="EXISTENCE:direct assay:GO:0035649 Nrd1
complex [PMID:10655211]"
/experiment="EXISTENCE:direct assay:GO:0071041 antisense
RNA transcript catabolic process [PMID:27190003]"
/experiment="EXISTENCE:mutant phenotype:GO:0031124 mRNA
3'-end processing [PMID:11565036]"
/experiment="EXISTENCE:mutant phenotype:GO:0031126
sno(s)RNA 3'-end processing [PMID:11565036]"
/experiment="EXISTENCE:mutant phenotype:GO:0034472 snRNA
3'-end processing [PMID:11565036]"
/experiment="EXISTENCE:mutant phenotype:GO:0042780 tRNA
3'-end processing [PMID:21460797]"
/experiment="EXISTENCE:mutant phenotype:GO:0071028 nuclear
mRNA surveillance [PMID:21113025]"
/experiment="EXISTENCE:mutant phenotype:GO:0071034 CUT
catabolic process [PMID:16973436]"
/experiment="EXISTENCE:mutant phenotype:GO:0071041
antisense RNA transcript catabolic process
[PMID:22028667]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:27076633]"
/note="RNA-binding subunit of Nrd1 complex; complex
interacts with exosome to mediate 3'-end formation of some
mRNAs, snRNAs, snoRNAs, and CUTs; interacts with CTD of
RNA pol II large subunit Rpo21p at phosphorylated Ser5 to
direct transcription termination of non-polyadenylated
transcripts; H3K4 trimethylation of transcribed regions by
Set1p enhances recruitment of Nrd1p to those sites; role
in regulation of mitochondrial abundance and cell size"
/codon_start=1
/product="Nrd1 complex RNA-binding subunit"
/protein_id="NP_014148.1"
/db_xref="GeneID:855470"
/db_xref="SGD:S000005195"
/translation="MQQDDDFQNFVATLESFKDLKSGISGSRIKKLTTYALDHIDIES
KIISLIIDYSRLCPDSHKLGSLYIIDSIGRAYLDETRSNSNSSSNKPGTCAHAINTLG
EVIQELLSDAIAKSNQDHKEKIRMLLDIWDRSGLFQKSYLNAIRSKCFAMDISNNTAN
TASQQLSLDPKQRSKQILSNLKKSPPLNLNISLPTDLTSTDPAKQQAALFQVIAALQK
HFKTLPSHTSVGTVAPPQAHTITEYGSRRERERERERYNSRRNRSRSPPAPFSQPSTG
RKDRYPSVAQDQYSIGAPNTTFGTNNHHLYPDELNVSNNPHYRPKPVSYDSTLPPDHI
KVYSRTLFIGGVPLNMKEWDLANVLKPFAEVQSVILNNSRKHAFVKVYSRHEAENVLQ
NFNKDGALPLRTRWGVGFGPRDCCDYQHGYSIIPMHRLTDADKKWSVSAQWGGTSGQP
LVTGIVFEEPDIIVGEGVSSKAISQKMPTDSGRNGPRSGKPNKSGSISSISPVPYGNA
PLASPPPQQYVQPMMQQPYGYAPNQPLPSQGPAAAAPPVPQQQFDPTAQLNSLMNMLN
QQQQQQQQS"
gene <175410..>179348
/gene="RAD50"
/locus_tag="YNL250W"
/db_xref="GeneID:855471"
mRNA <175410..>179348
/gene="RAD50"
/locus_tag="YNL250W"
/product="MRX complex DNA-binding subunit"
/transcript_id="NM_001183088.1"
/db_xref="GeneID:855471"
CDS 175410..179348
/gene="RAD50"
/locus_tag="YNL250W"
/experiment="EXISTENCE:direct assay:GO:0003690
double-stranded DNA binding [PMID:8367302]"
/experiment="EXISTENCE:direct assay:GO:0003691
double-stranded telomeric DNA binding [PMID:17698079]"
/experiment="EXISTENCE:direct assay:GO:0004017 AMP kinase
activity [PMID:17349953]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922|PMID:9845372]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:15546877]"
/experiment="EXISTENCE:direct assay:GO:0042162 telomeric
DNA binding [PMID:15721260]"
/experiment="EXISTENCE:direct assay:GO:0043047
single-stranded telomeric DNA binding [PMID:17698079]"
/experiment="EXISTENCE:direct assay:GO:0051880
G-quadruplex DNA binding [PMID:17698079]"
/experiment="EXISTENCE:mutant phenotype:GO:0000722
telomere maintenance via recombination [PMID:11238918]"
/experiment="EXISTENCE:mutant phenotype:GO:0000723
telomere maintenance [PMID:17349953]"
/experiment="EXISTENCE:mutant phenotype:GO:0004017 AMP
kinase activity [PMID:17349953]"
/experiment="EXISTENCE:mutant phenotype:GO:0006284
base-excision repair [PMID:20040573]"
/experiment="EXISTENCE:mutant phenotype:GO:0006303
double-strand break repair via nonhomologous end joining
[PMID:12399380|PMID:9501103|PMID:31390569]"
/experiment="EXISTENCE:mutant phenotype:GO:0007129
homologous chromosome pairing at meiosis [PMID:8207053]"
/experiment="EXISTENCE:mutant phenotype:GO:0035753
maintenance of DNA trinucleotide repeats [PMID:27173583]"
/experiment="EXISTENCE:mutant phenotype:GO:0051321 meiotic
cell cycle [PMID:17349953]"
/experiment="EXISTENCE:mutant phenotype:GO:0097552
mitochondrial double-strand break repair via homologous
recombination [PMID:22214610]"
/experiment="EXISTENCE:physical interaction:GO:0030870
Mre11 complex [PMID:9845372]"
/note="Subunit of MRX complex with Mre11p and Xrs2p;
complex is involved in processing double-strand DNA breaks
in vegetative cells, initiation of meiotic DSBs, telomere
maintenance, and nonhomologous end joining; forms nuclear
foci upon DNA replication stress"
/codon_start=1
/product="MRX complex DNA-binding subunit"
/protein_id="NP_014149.1"
/db_xref="GeneID:855471"
/db_xref="SGD:S000005194"
/translation="MSAIYKLSIQGIRSFDSNDRETIEFGKPLTLIVGMNGSGKTTII
ECLKYATTGDLPPNSKGGVFIHDPKITGEKDIRAQVKLAFTSANGLNMIVTRNIQLLM
KKTTTTFKTLEGQLVAINNSGDRSTLSTRSLELDAQVPLYLGVPKAILEYVIFCHQED
SLWPLSEPSNLKKKFDEIFQAMKFTKALDNLKSIKKDMSVDIKLLKQSVEHLKLDKDR
SKAMKLNIHQLQTKIDQYNEEVSEIESQLNEITEKSDKLFKSNQDFQKILSKVENLKN
TKLSISDQVKRLSNSIDILDLSKPDLQNLLANFSKVLMDKNNQLRDLETDISSLKDRQ
SSLQSLSNSLIRRQGELEAGKETYEKNRNHLSSLKEAFQHKFQGLSNIENSDMAQVNH
EMSQFKAFISQDLTDTIDQFAKDIQLKETNLSDLIKSITVDSQNLEYNKKDRSKLIHD
SEELAEKLKSFKSLSTQDSLNHELENLKTYKEKLQSWESENIIPKLNQKIEEKNNEMI
ILENQIEKFQDRIMKTNQQADLYAKLGLIKKSINTKLDELQKITEKLQNDSRIRQVFP
LTQEFQRADLEMDFQKLFINMQKNIAINNKKMHELDRRYTNALYNLNTIEKDLQDNQK
SKEKVIQLLSENLPEDCTIDEYNDVLEETELSYKTALENLKMHQTTLEFNRKALEIAE
RDSCCYLCSRKFENESFKSKLLQELKTKTDANFEKTLKDTVQNEKEYLHSLRLLEKHI
ITLNSINEKIDNSQKCLEKAKEETKTSKSKLDELEVDSTKLKDEKELAESEIRPLIEK
FTYLEKELKDLENSSKTISEELSIYNTSEDGIQTVDELRDQQRKMNDSLRELRKTISD
LQMEKDEKVRENSRMINLIKEKELTVSEIESSLTQKQNIDDSIRSKRENINDIDSRVK
ELEARIISLKNKKDEAQSVLDKVKNERDIQVRNKQKTVADINRLIDRFQTIYNEVVDF
EAKGFDELQTTIKELELNKAQMLELKEQLDLKSNEVNEEKRKLADSNNEEKNLKQNLE
LIELKSQLQHIESEISRLDVQNAEAERDKYQEESLRLRTRFEKLSSENAGKLGEMKQL
QNQIDSLTHQLRTDYKDIEKNYHKEWVELQTRSFVTDDIDVYSKALDSAIMKYHGLKM
QDINRIIDELWKRTYSGTDIDTIKIRSDEVSSTVKGKSYNYRVVMYKQDVELDMRGRC
SAGQKVLASIIIRLALSETFGANCGVIALDEPTTNLDEENIESLAKSLHNIINMRRHQ
KNFQLIVITHDEKFLGHMNAAAFTDHFFKVKRDDRQKSQIEWVDINRVTY"
gene complement(<179394..>181022)
/gene="MPA43"
/locus_tag="YNL249C"
/db_xref="GeneID:855472"
mRNA complement(<179394..>181022)
/gene="MPA43"
/locus_tag="YNL249C"
/product="Mpa43p"
/transcript_id="NM_001183087.1"
/db_xref="GeneID:855472"
CDS complement(179394..181022)
/gene="MPA43"
/locus_tag="YNL249C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/note="hypothetical protein; the authentic, non-tagged
protein is detected in highly purified mitochondria in
high-throughput studies"
/codon_start=1
/product="Mpa43p"
/protein_id="NP_014150.1"
/db_xref="GeneID:855472"
/db_xref="SGD:S000005193"
/translation="MNSSLQVGIGIDVGSSSARIGVYNYYNDALLEMAQEPVPYYQDS
SKKSWKFWQKSTEIIKALQKCLQKLNIREYEVKSCGVSATCSLAIFERDRTSNMLIPY
PNEDNVIFWMDSSAVNECQWLNMQCPQQLLDYLGGKFVPEMGVPKLKYFLDEYSHLRD
KHFHIFDLHQYIAYELSRLYEWNIEGLLGRENLNGIGNDGEVSGWSSSFYKNIINLPS
NVSIGTTSLVANKHISTTVVRSCIDSYASWFAVASPHLETSLFMIAGTSSCYMYGTTI
SDTRIPGVWGPFDTILDNRGDFSVYAAGQSCTGKLIEHLFESHPCARKILKDGADIYQ
VLEQTIRDIEKNNGLSIHILTKDMFFYGDYEGNRTPFADPRIKGSFIGESTDTSMLNL
TYKYICILEFLSFQTKLIIDTFQNENSNIHIKELRISGSQAKNERLLSLISLVNNGVA
IIKPKENVDMMGIKGAYVLAKSAKEKKQLADVITERDISNDSEKFESLAEYRLGNDSI
LLRKLLCVKYHIHLDMAKQQKRYHKLVDEVFQHL"
gene complement(<181361..>182608)
/gene="RPA49"
/locus_tag="YNL248C"
/db_xref="GeneID:855473"
mRNA complement(<181361..>182608)
/gene="RPA49"
/locus_tag="YNL248C"
/product="DNA-directed RNA polymerase I subunit RPA49"
/transcript_id="NM_001183086.1"
/db_xref="GeneID:855473"
CDS complement(181361..182608)
/gene="RPA49"
/locus_tag="YNL248C"
/experiment="EXISTENCE:direct assay:GO:0003899
DNA-directed RNA polymerase activity [PMID:9837969]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:21263028]"
/experiment="EXISTENCE:direct assay:GO:0005736 RNA
polymerase I complex [PMID:9837969|PMID:11486042]"
/experiment="EXISTENCE:direct assay:GO:0006360
transcription by RNA polymerase I [PMID:9837969]"
/experiment="EXISTENCE:direct assay:GO:0006362
transcription elongation by RNA polymerase I
[PMID:18160037]"
/experiment="EXISTENCE:genetic interaction:GO:0042790
nucleolar large rRNA transcription by RNA polymerase I
[PMID:9121426]"
/experiment="EXISTENCE:mutant phenotype:GO:0005736 RNA
polymerase I complex [PMID:1409638]"
/experiment="EXISTENCE:mutant phenotype:GO:0008361
regulation of cell size [PMID:12089449]"
/experiment="EXISTENCE:mutant phenotype:GO:0042790
nucleolar large rRNA transcription by RNA polymerase I
[PMID:12374750]"
/note="RNA polymerase I subunit A49; essential for
nucleolar assembly and for high polymerase loading rate;
required for nucleolar localization of Rpa34p"
/codon_start=1
/product="DNA-directed RNA polymerase I subunit RPA49"
/protein_id="NP_014151.1"
/db_xref="GeneID:855473"
/db_xref="SGD:S000005192"
/translation="MSVKRSVSEIEIESVQDQPSVAVGSFFKGFRAPSDTTFDLYKKK
KSEKDEFVLHGENERLEYEGYTDSSSQASNQYVVGLFNPEKKSIQLYKAPVLVSKVVS
KSSKNLRGPKIKSKSDTRPSALRNALGEAFGTKKAKKAIADLERNRIDSDKLTDSAID
IVDSVRTASKDLPTRAQLDEITSNDRPTPLANIDATDVEQIYPIESIIPKKELQFIRV
SSILKEADKEKKLELFPYQNNSKYVAKKLDSLTQPSQMTKLQLLYYLSLLLGVYENRR
VNNKTKLLERLNSPPEILVDGILSRFTVIKPGQFGRSKDRSYFIDPQNEDKILCYILA
IIMHLDNFIVEITPLAHELNLKPSKVVSLFRVLGAIVKGATVAQAEAFGIPKSTAASY
KIATMKVPFKLPEMTRRGRGPRR"
gene <182875..>185178
/gene="CRS1"
/locus_tag="YNL247W"
/db_xref="GeneID:855474"
mRNA <182875..>185178
/gene="CRS1"
/locus_tag="YNL247W"
/product="cysteine--tRNA ligase"
/transcript_id="NM_001183085.1"
/db_xref="GeneID:855474"
CDS 182875..185178
/gene="CRS1"
/locus_tag="YNL247W"
/EC_number="6.1.1.16"
/experiment="EXISTENCE:direct assay:GO:0004817
cysteine-tRNA ligase activity [PMID:9523015]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:31350340]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:31350340]"
/experiment="EXISTENCE:direct assay:GO:0006423
cysteinyl-tRNA aminoacylation [PMID:9523015]"
/experiment="EXISTENCE:direct assay:GO:1990825
sequence-specific mRNA binding [PMID:34039240]"
/note="Cysteinyl-tRNA synthetase; CRS1 has two in-frame
start codons resulting in a shorter isoform that is
retained in the cytosol and a longer form that is
mitochondrial; may interact with ribosomes, based on
co-purification experiments; human gene CARS allows growth
of the yeast haploid null mutant after sporulation of a
heterozygous diploid"
/codon_start=1
/product="cysteine--tRNA ligase"
/protein_id="NP_014152.1"
/db_xref="GeneID:855474"
/db_xref="SGD:S000005191"
/translation="MNIFIKALRRYTIMSTPKIVQPKWKVPTPQAKETVLKLYNSLTR
SKVEFIPQSGNRGVTWYSCGPTVYDASHMGHARNYVSIDINRRIIQDYFGYDVQFVQN
VTDIDDKIILRARQNYLFDNFVKENDTKFNATVVDKVKTALFQYINKNFTIQGSEIKT
IEEFETWLSNADTETLKLENPKFPMHVTAVQNAIESITKGDSMDAEVAFEKVKDVTVP
LLDKELGSTISNPEIFRQLPAYWEQKFNDDMLSLNVLPPTVTTRVSEYVPEIIDFVQK
IIDNGYAYATSDGSVYFDTLKFDKSPNHDYAKCQPWNKGQLDLINDGEGSLSNFADNG
KKSNNDFALWKASKAGEPEWESPWGKGRPGWHIECSVMASDILGSNIDIHSGGIDLAF
PHHDNELAQSEARFDNQQWINYFLHTGHLHIEGQKMSKSLKNFITIQEALKKFSPRQL
RLAFASVQWNNQLDFKESLIHEVKSFENSMNNFFKTIRALKNDAASAGHISKKFSPLE
KELLADFVESESKVHSAFCDNLSTPVALKTLSELVTKSNTYITTAGAALKIEPLIAIC
SYITKILRIIGFPSRPDNLGWAAQAGSNDGSLGSLEDTVMPYVKCLSTFRDDVRSLAI
KKAEPKEFLQLTDKIRNEDLLNLNVALDDRNGQSALIKFLTNDEKLEIVKLNEEKHAN
ELAKKQKKLEQQKLREQKENERKQKAQIKPQDMFKDVTLYSAWDEQGLPTKDKDGNDI
TKSMTKKLKKQWEQQKKLHEEYFGEDK"
gene <185460..>186349
/gene="VPS75"
/locus_tag="YNL246W"
/db_xref="GeneID:855475"
mRNA join(<185460..185491,185587..>186349)
/gene="VPS75"
/locus_tag="YNL246W"
/product="Vps75p"
/transcript_id="NM_001183084.1"
/db_xref="GeneID:855475"
CDS join(185460..185491,185587..186349)
/gene="VPS75"
/locus_tag="YNL246W"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:17344218]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0006334 nucleosome
assembly [PMID:17344218]"
/experiment="EXISTENCE:direct assay:GO:0010698
acetyltransferase activator activity [PMID:19172749]"
/experiment="EXISTENCE:direct assay:GO:0042393 histone
binding [PMID:17344218]"
/experiment="EXISTENCE:mutant phenotype:GO:0006303
double-strand break repair via nonhomologous end joining
[PMID:18036332]"
/experiment="EXISTENCE:mutant phenotype:GO:0010698
acetyltransferase activator activity [PMID:19172749]"
/experiment="EXISTENCE:physical interaction:GO:0006303
double-strand break repair via nonhomologous end joining
[PMID:18036332]"
/experiment="EXISTENCE:physical interaction:GO:0042393
histone binding [PMID:17344218]"
/note="NAP family histone chaperone; binds to histones and
Rtt109p, stimulating histone acetyltransferase activity;
possesses nucleosome assembly activity in vitro; proposed
role in vacuolar protein sorting and in double-strand
break repair; protein abundance increases in response to
DNA replication stress; relocalizes to the cytosol in
response to hypoxia"
/codon_start=1
/product="Vps75p"
/protein_id="NP_014153.1"
/db_xref="GeneID:855475"
/db_xref="SGD:S000005190"
/translation="MMSDQENENEHAKAFLGLAKCEEEVDAIEREVELYRLNKMKPVY
EKRDAYIDEIAEFWKIVLSQHVSFANYIRASDFKYIDTIDKIKVEWLALESEMYDTRD
FSITFHFHGIEGDFKEQQVTKVFQIKKGKDDQEDGILTSEPVPIEWPQSYDSINPDLI
KDKRSPEGKKKYRQGMKTIFGWFRWTGLKPGKEFPHGDSLASLFSEEIYPFCVKYYAE
AQRDLEDEEGESGLSADGDSEDDDGSLGEVDLPLSDEEPSSKKRKV"
gene complement(<186346..>186885)
/gene="CWC25"
/locus_tag="YNL245C"
/db_xref="GeneID:855476"
mRNA complement(<186346..>186885)
/gene="CWC25"
/locus_tag="YNL245C"
/product="U2-type spliceosomal complex subunit CWC25"
/transcript_id="NM_001183083.1"
/db_xref="GeneID:855476"
CDS complement(186346..186885)
/gene="CWC25"
/locus_tag="YNL245C"
/experiment="EXISTENCE:direct assay:GO:0000384 first
spliceosomal transesterification activity [PMID:37858289]"
/experiment="EXISTENCE:direct assay:GO:0000398 mRNA
splicing, via spliceosome [PMID:19935684]"
/experiment="EXISTENCE:direct assay:GO:0005684 U2-type
spliceosomal complex [PMID:11884590]"
/note="Splicing factor required for the first step of
pre-mRNA splicing; binding to the spliceosome requires
Prp2p and Yju2p; heat-stable protein; has similarity to S.
pombe Cwf25p"
/codon_start=1
/product="U2-type spliceosomal complex subunit CWC25"
/protein_id="NP_014154.1"
/db_xref="GeneID:855476"
/db_xref="SGD:S000005189"
/translation="MGSGDLNLLKSWNPKLMKNRKKVWETEQDLITEQQKLNTRLKEI
EKERELNELLNESSKDKPETLKNDLALKKSGLEWMYQDAKLSDEKEDYLLGKKKLDSS
ILNQPATPPVRAATTISASGAATSISSQKKKSKLLKDDPMSKFKVTKQQRRTPDSTKK
RAMSQRGKPLSKPAPDLDY"
gene complement(<187170..>187496)
/gene="SUI1"
/locus_tag="YNL244C"
/gene_synonym="MOF2; RFR1"
/db_xref="GeneID:855477"
mRNA complement(<187170..>187496)
/gene="SUI1"
/locus_tag="YNL244C"
/gene_synonym="MOF2; RFR1"
/product="translation initiation factor eIF1"
/transcript_id="NM_001183082.1"
/db_xref="GeneID:855477"
CDS complement(187170..187496)
/gene="SUI1"
/locus_tag="YNL244C"
/gene_synonym="MOF2; RFR1"
/experiment="EXISTENCE:direct assay:GO:0001731 formation
of translation preinitiation complex
[PMID:15664195|PMID:12008673|PMID:17434125|PMID:17504939]"
/experiment="EXISTENCE:direct assay:GO:0003743 translation
initiation factor activity [PMID:12008673]"
/experiment="EXISTENCE:direct assay:GO:0016282 eukaryotic
43S preinitiation complex [PMID:12008673]"
/experiment="EXISTENCE:direct assay:GO:0031369 translation
initiation factor binding [PMID:12861028|PMID:15145951]"
/experiment="EXISTENCE:direct assay:GO:0043024 ribosomal
small subunit binding
[PMID:12860115|PMID:19751744|PMID:17434125]"
/experiment="EXISTENCE:direct assay:GO:0043614 multi-eIF
complex [PMID:15838098|PMID:11018020]"
/experiment="EXISTENCE:mutant phenotype:GO:0001731
formation of translation preinitiation complex
[PMID:19751744|PMID:17504939]"
/experiment="EXISTENCE:mutant phenotype:GO:0006450
regulation of translational fidelity [PMID:19751744]"
/experiment="EXISTENCE:mutant phenotype:GO:1990145
maintenance of translational fidelity [PMID:9488467]"
/experiment="EXISTENCE:physical interaction:GO:0031369
translation initiation factor binding
[PMID:12861028|PMID:15145951|PMID:22851688]"
/note="Translation initiation factor eIF1; component of
complex involved in recognition of initiator codon;
modulates translation accuracy at initiation phase by
discriminating against suboptimal initiation sites to
prevent excessive uORF translation genome-wide"
/codon_start=1
/product="translation initiation factor eIF1"
/protein_id="NP_014155.1"
/db_xref="GeneID:855477"
/db_xref="SGD:S000005188"
/translation="MSIENLKSFDPFADTGDDETATSNYIHIRIQQRNGRKTLTTVQG
VPEEYDLKRILKVLKKDFACNGNIVKDPEMGEIIQLQGDQRAKVCEFMISQLGLQKKN
IKIHGF"
gene <188051..>190957
/gene="SLA2"
/locus_tag="YNL243W"
/gene_synonym="END4; MOP2"
/db_xref="GeneID:855478"
mRNA <188051..>190957
/gene="SLA2"
/locus_tag="YNL243W"
/gene_synonym="END4; MOP2"
/product="Sla2p"
/transcript_id="NM_001183081.2"
/db_xref="GeneID:855478"
CDS 188051..190957
/gene="SLA2"
/locus_tag="YNL243W"
/gene_synonym="END4; MOP2"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:11302750]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0030479 actin
cortical patch [PMID:10397764]"
/experiment="EXISTENCE:direct assay:GO:0034316 negative
regulation of Arp2/3 complex-mediated actin nucleation
[PMID:17151356]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807]"
/experiment="EXISTENCE:mutant phenotype:GO:0000147 actin
cortical patch assembly [PMID:8335689]"
/experiment="EXISTENCE:mutant phenotype:GO:0006897
endocytosis [PMID:8380177]"
/experiment="EXISTENCE:mutant phenotype:GO:0007015 actin
filament organization [PMID:10397764]"
/note="Adaptor protein that links actin to clathrin and
endocytosis; involved in membrane cytoskeleton assembly
and cell polarization; present in the actin cortical patch
of the emerging bud tip; dimer in vivo"
/codon_start=1
/product="Sla2p"
/protein_id="NP_014156.2"
/db_xref="GeneID:855478"
/db_xref="SGD:S000005187"
/translation="MSRIDSDLQKALKKACSVEETAPKRKHVRACIVYTWDHQSSKAV
FTTLKTLPLANDEVQLFKMLIVLHKIIQEGHPSALAEAIRDRDWIRSLGRVHSGGSSY
SKLIREYVRYLVLKLDFHAHHRGFNNGTFEYEEYVSLVSVSDPDEGYETILDLMSLQD
SLDEFSQIIFASIQSERRNTECKISALIPLIAESYGIYKFITSMLRAMHRQLNDAEGD
AALQPLKERYELQHARLFEFYADCSSVKYLTTLVTIPKLPVDAPDVFLINDVDESKEI
KFKKREPSVTPARTPARTPTPTPPVVAEPAISPRPVSQRTTSTPTGYLQTMPTGATTG
MMIPTATGAANAIFPQATAQMQPDFWANQQAQFANEQNRLEQERVQQLQQQQAQQELF
QQQLQKAQQDMMNMQLQQQNQHQNDLIALTNQYEKDQALLQQYDQRVQQLESEITTMD
STASKQLANKDEQLTALQDQLDVWERKYESLAKLYSQLRQEHLNLLPRFKKLQLKVNS
AQESIQKKEQLEHKLKQKDLQMAELVKDRDRARLELERSINNAEADSAAATAAAETMT
QDKMNPILDAILESGINTIQESVYNLDSPLSWSGPLTPPTFLLSLLESTSENATEFAT
SFNNLIVDGLAHGDQTEVIHCVSDFSTSMATLVTNSKAYAVTTLPQEQSDQILTLVKR
CAREAQYFFEDLMSENLNQVGDEEKTDIVINANVDMQEKLQELSLAIEPLLNIQSVKS
NKETNPHSELVATADKIVKSSEHLRVDVPKPLLSLALMIIDAVVALVKAAIQCQNEIA
TTTSIPLNQFYLKNSRWTEGLISAAKAVAGATNVLITTASKLITSEDNENTSPEQFIV
ASKEVAASTIQLVAASRVKTSIHSKAQDKLEHCSKDVTDACRSLGNHVMGMIEDDHST
SQQQQPLDFTSEHTLKTAEMEQQVEILKLEQSLSNARKRLGEIRRHAYYNQDDD"
gene <191324..>196102
/gene="ATG2"
/locus_tag="YNL242W"
/gene_synonym="APG2; AUT8; SPO72"
/db_xref="GeneID:855479"
mRNA <191324..>196102
/gene="ATG2"
/locus_tag="YNL242W"
/gene_synonym="APG2; AUT8; SPO72"
/product="Atg2p"
/transcript_id="NM_001183080.1"
/db_xref="GeneID:855479"
CDS 191324..196102
/gene="ATG2"
/locus_tag="YNL242W"
/gene_synonym="APG2; AUT8; SPO72"
/experiment="EXISTENCE:direct assay:GO:0000407 phagophore
assembly site [PMID:18497569|PMID:11689437]"
/experiment="EXISTENCE:direct assay:GO:0000425 pexophagy
[PMID:11382760]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922|PMID:11382760]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0032266
phosphatidylinositol-3-phosphate binding [PMID:27498190]"
/experiment="EXISTENCE:direct assay:GO:0061908 phagophore
[PMID:23549786]"
/experiment="EXISTENCE:mutant phenotype:GO:0000045
autophagosome assembly [PMID:11382761]"
/experiment="EXISTENCE:mutant phenotype:GO:0000422
autophagy of mitochondrion [PMID:19793921]"
/experiment="EXISTENCE:mutant phenotype:GO:0000425
pexophagy [PMID:11382760]"
/experiment="EXISTENCE:mutant phenotype:GO:0006914
autophagy [PMID:23382696]"
/experiment="EXISTENCE:mutant phenotype:GO:0032258
cytoplasm to vacuole targeting by the Cvt pathway
[PMID:11382761]"
/experiment="EXISTENCE:mutant phenotype:GO:0034727
piecemeal microautophagy of the nucleus [PMID:18701704]"
/experiment="EXISTENCE:mutant phenotype:GO:0044804
nucleophagy [PMID:22768199]"
/experiment="EXISTENCE:mutant phenotype:GO:0061709
reticulophagy [PMID:26181331]"
/note="Putative lipid transport protein; peripheral
membrane protein required for vesicle formation during
autophagy, pexophagy and the CVT pathway; directed to
autophagic membranes by Atg18p where it acts as a PAS-ER
membrane tether during autophagosome formation; involved
in Atg9p cycling between the PAS and mitochondria; binds
the phospholipid PI(3)P via an APT1 domain; essential for
cell cycle progression from G2/M to G1 under nitrogen
starvation; forms cytoplasmic foci upon DNA replication
stress"
/codon_start=1
/product="Atg2p"
/protein_id="NP_014157.1"
/db_xref="GeneID:855479"
/db_xref="SGD:S000005186"
/translation="MAFWLPQNIQKRLLLYVLQQISLFSNIDLSNLDVSIGSKSHFSF
HDVNLSLDDLNIPNVQINEGIVDELVLKLTVSGGVEIDGSGLRFIMTPLYSSGSQELH
SDFLVKSIQDLTNSMLQFSDPLTTYNRYKEDDISSSDSSSDLNSNIEASKPAANGSYT
LQNMRNKALNVALAKLKIALKDVTIRFIVNDRDPSDNIVEVHLESIQLITTDANLRHI
NIENITISSIQKQAVPDSPVHPFNNDDLSQSVYLSKMEATSLYMSAMEEQSNEDPSEP
QVTQEEQENDKCKESLMEINNLNIAFKGLSSVNDLRMSNIVIDIQDVHLAIHKIVEIK
NSTLKNIIDIIVTHLDANESFSCQDSQSPSPDKQEPSALSSVDIKCIYLNLGQDITVI
LKSFKLEQKENNSLAFSLGSFYSNSSPLTISHKTKPLLTGEQTPQSIALNMGDELDII
ISHDGIAHFFKIFQFVSKCMSFYQNKSKGMMPQIASDTKRTVQLTSKAVKLSLKFPYF
LLCFQVSPFIYDSNRELYIELVDVFKKLPSRCTKILTMSSITISNLQSPLQLGSYDDT
LKEALIYSSVHAIIKEVIFNEEYSGIVQLVEDISAFGKLFTDSKNSECTGKSKSKRGS
FLQRSVRVLNSSRFVYKQSLSANFSLKIDSMKLKVSEIIGPQFGSVEALLSNNFFAIT
DDSQIVYFTKNLKVERKTPSLLEPQEIMSVVLNKAVNEPVLYVHRRANGKLKVIFNNI
RIHYYARWLEILKKNIGPDNASSKDEPVSQKLSKKQPTSGFPWELKCLDCSLILHPFR
LKSVMVIVLDNLTTGGSSFIPQAKLLSKANTLFLIDDYQNFKIQKDKNWPSLINFYAG
QGFSAIGKIDTLNFLINKSDGALLLDCKIEQVGLSLCADSFQTFCQLCIDLKYPQTFP
DEEKFRTQLKNPIDVFKDIDCDLFNSAFIRENNHQNDYDSVHLVDSFLDKTHEFNNGA
RSKLSSQGSYEMDSSSGTATGGILLPHESYLDSAQPKEEDTPPIASKEQERDVDIRGS
IDVEKVVIKLFDGYDWKYTRKFIANTVEKLDKELSKAEASSSKSNVPQSEANIFDSIY
ISANKNNVTDLRRNLDGEIQGVQNSFSDVSKVNLRPSKHYKALIQLNKVHVNLKNYRV
DEPDESNSDNSTDVLNRCVVSIYEFEIIDNVPTSTWNKFVTLLKHEPWPHSSPMFLLD
LEFIRPIDFLQAVELVMQLNVAPLRLHVDQDTLEFLIRFLGFKDKRFELIDEYPDIVF
IQKFSTNSIKLRLDYKPKKVDYAGLRSGQTSELMNFFTLDGSKIILKSVVLYGLNGFD
ELNNKLKAIWTPDITKKQLPGVLEGLAPVRSFMAIGSGVKTLVTVLMSEYRQEGHLGR
SLKKGGNVFLKTTTGDFVKLGVKLTSGTQAILENTEELFGGVGSNGRVYDASKFGSAD
GADSDTAAVLDLDTLFEEDQLVGSKYSRIRDHEPTAVVIDMSSPGDHNEPTIVSLYAD
QPLDLPTGLKEAYSSLEKHMHIAYDAVWRAKGQMKDDKRGGPSAAAVYVARAAPVAII
RPLIGATEAVSKTLQGIANQVDKTHNEQINDKYKSNRTDS"
rep_origin 196134..196232
/note="ARS1412; ARS activated in late S phase"
/db_xref="SGD:S000077374"
gene complement(<196426..>197943)
/gene="ZWF1"
/locus_tag="YNL241C"
/gene_synonym="MET19; POS10"
/db_xref="GeneID:855480"
mRNA complement(<196426..>197943)
/gene="ZWF1"
/locus_tag="YNL241C"
/gene_synonym="MET19; POS10"
/product="glucose-6-phosphate dehydrogenase"
/transcript_id="NM_001183079.1"
/db_xref="GeneID:855480"
CDS complement(196426..197943)
/gene="ZWF1"
/locus_tag="YNL241C"
/gene_synonym="MET19; POS10"
/EC_number="1.1.1.49"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:11914276]"
/experiment="EXISTENCE:mutant phenotype:GO:0004345
glucose-6-phosphate dehydrogenase activity
[PMID:2269430|PMID:2001672]"
/experiment="EXISTENCE:mutant phenotype:GO:0006740 NADPH
regeneration [PMID:2269430]"
/experiment="EXISTENCE:mutant phenotype:GO:0009051
pentose-phosphate shunt, oxidative branch
[PMID:8299150|PMID:7045591]"
/experiment="EXISTENCE:mutant phenotype:GO:0042542
response to hydrogen peroxide [PMID:7586028|PMID:9480895]"
/note="Glucose-6-phosphate dehydrogenase (G6PD); catalyzes
the first step of the pentose phosphate pathway; involved
in adaptation to oxidative stress; negative regulator of
nonselective autophagy, independent of Stb5p; protein
abundance increases in response to DNA replication stress;
homolog of human G6PD which is deficient in patients with
hemolytic anemia; human G6PD can complement yeast zwf1
null mutant"
/codon_start=1
/product="glucose-6-phosphate dehydrogenase"
/protein_id="NP_014158.1"
/db_xref="GeneID:855480"
/db_xref="SGD:S000005185"
/translation="MSEGPVKFEKNTVISVFGASGDLAKKKTFPALFGLFREGYLDPS
TKIFGYARSKLSMEEDLKSRVLPHLKKPHGEADDSKVEQFFKMVSYISGNYDTDEGFD
ELRTQIEKFEKSANVDVPHRLFYLALPPSVFLTVAKQIKSRVYAENGITRVIVEKPFG
HDLASARELQKNLGPLFKEEELYRIDHYLGKELVKNLLVLRFGNQFLNASWNRDNIQS
VQISFKERFGTEGRGGYFDSIGIIRDVMQNHLLQIMTLLTMERPVSFDPESIRDEKVK
VLKAVAPIDTDDVLLGQYGKSEDGSKPAYVDDDTVDKDSKCVTFAAMTFNIENERWEG
VPIMMRAGKALNESKVEIRLQYKAVASGVFKDIPNNELVIRVQPDAAVYLKFNAKTPG
LSNATQVTDLNLTYASRYQDFWIPEAYEVLIRDALLGDHSNFVRDDELDISWGIFTPL
LKHIERPDGPTPEIYPYGSRGPKGLKEYMQKHKYVMPEKHPYAWPVTKPEDTKDN"
gene complement(<198502..>199977)
/gene="NAR1"
/locus_tag="YNL240C"
/db_xref="GeneID:855481"
mRNA complement(<198502..>199977)
/gene="NAR1"
/locus_tag="YNL240C"
/product="iron-sulfur cluster assembly protein NAR1"
/transcript_id="NM_001183078.1"
/db_xref="GeneID:855481"
CDS complement(198502..199977)
/gene="NAR1"
/locus_tag="YNL240C"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:15103330]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:15103330]"
/experiment="EXISTENCE:mutant phenotype:GO:0016226
iron-sulfur cluster assembly [PMID:15103330]"
/note="Subunit of the cytosolic iron-sulfur (FeS) protein
assembly machinery; required for maturation of cytosolic
and nuclear FeS proteins and for normal resistance to
oxidative stress; deficiency results in shortened lifespan
and sensitivity to paraquat; homologous to human Narf"
/codon_start=1
/product="iron-sulfur cluster assembly protein NAR1"
/protein_id="NP_014159.1"
/db_xref="GeneID:855481"
/db_xref="SGD:S000005184"
/translation="MSALLSESDLNDFISPALACVKPTQVSGGKKDNVNMNGEYEVST
EPDQLEKVSITLSDCLACSGCITSSEEILLSSQSHSVFLKNWGKLSQQQDKFLVVSVS
PQCRLSLAQYYGLTLEAADLCLMNFFQKHFQCKYMVGTEMGRIISISKTVEKIIAHKK
QKENTGADRKPLLSAVCPGFLIYTEKTKPQLVPMLLNVKSPQQITGSLIRATFESLAI
ARESFYHLSLMPCFDKKLEASRPESLDDGIDCVITPREIVTMLQELNLDFKSFLTEDT
SLYGRLSPPGWDPRVHWASNLGGTCGGYAYQYVTAVQRLHPGSQMIVLEGRNSDIVEY
RLLHDDRIIAAASELSGFRNIQNLVRKLTSGSGSERKRNITALRKRRTGPKANSREMA
AATAATADPYHSDYIEVNACPGACMNGGGLLNGEQNSLKRKQLVQTLNKRHGEELAMV
DPLTLGPKLEEAAARPLSLEYVFAPVKQAVEKDLVSVGSTW"
gene <200569..>201933
/gene="LAP3"
/locus_tag="YNL239W"
/gene_synonym="BLH1; GAL6; YCP1"
/db_xref="GeneID:855482"
mRNA <200569..>201933
/gene="LAP3"
/locus_tag="YNL239W"
/gene_synonym="BLH1; GAL6; YCP1"
/product="bleomycin hydrolase"
/transcript_id="NM_001183077.1"
/db_xref="GeneID:855482"
CDS 200569..201933
/gene="LAP3"
/locus_tag="YNL239W"
/gene_synonym="BLH1; GAL6; YCP1"
/EC_number="3.4.22.40"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:8063738]"
/experiment="EXISTENCE:direct assay:GO:0003690
double-stranded DNA binding [PMID:9584198]"
/experiment="EXISTENCE:direct assay:GO:0003697
single-stranded DNA binding [PMID:9584198]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:21124907]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:19061865|PMID:11914276|PMID:9584198|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:14576278|PMID:16823961|PMID:14562095|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0008234
cysteine-type peptidase activity [PMID:8063738]"
/experiment="EXISTENCE:direct assay:GO:0043418
homocysteine catabolic process [PMID:16769724]"
/experiment="EXISTENCE:direct assay:GO:0046677 response to
antibiotic [PMID:8063738]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:9374524]"
/experiment="EXISTENCE:mutant phenotype:GO:0043418
homocysteine catabolic process [PMID:16769724]"
/note="Cysteine aminopeptidase with
homocysteine-thiolactonase activity; protects cells
against homocysteine toxicity; cytosolic protein,
selectively transported to vacuole during nitrogen
starvation; targeted to vacuole via CVT pathway; has
bleomycin hydrolase activity in vitro; transcription is
regulated by galactose via Gal4p; orthologous to human
BLMH"
/codon_start=1
/product="bleomycin hydrolase"
/protein_id="NP_014160.2"
/db_xref="GeneID:855482"
/db_xref="SGD:S000005183"
/translation="MSSSIDISKINSWNKEFQSDLTHQLATTVLKNYNADDALLNKTR
LQKQDNRVFNTVVSTDSTPVTNQKSSGRCWLFAATNQLRLNVLSELNLKEFELSQAYL
FFYDKLEKANYFLDQIVSSADQDIDSRLVQYLLAAPTEDGGQYSMFLNLVKKYGLIPK
DLYGDLPYSTTASRKWNSLLTTKLREFAETLRTALKERSADDSIIVTLREQMQREIFR
LMSLFMDIPPVQPNEQFTWEYVDKDKKIHTIKSTPLEFASKYAKLDPSTPVSLINDPR
HPYGKLIKIDRLGNVLGGDAVIYLNVDNETLSKLVVKRLQNNKAVFFGSHTPKFMDKK
TGVMDIELWNYPAIGYNLPQQKASRIRYHESLMTHAMLITGCHVDETSKLPLRYRVEN
SWGKDSGKDGLYVMTQKYFEEYCFQIVVDINELPKELASKFTSGKEEPIVLPIWDPMG
ALAK"
gene <202428..>204872
/gene="KEX2"
/locus_tag="YNL238W"
/gene_synonym="QDS1; SRB1; VMA45"
/db_xref="GeneID:855483"
mRNA <202428..>204872
/gene="KEX2"
/locus_tag="YNL238W"
/gene_synonym="QDS1; SRB1; VMA45"
/product="kexin KEX2"
/transcript_id="NM_001183076.1"
/db_xref="GeneID:855483"
CDS 202428..204872
/gene="KEX2"
/locus_tag="YNL238W"
/gene_synonym="QDS1; SRB1; VMA45"
/EC_number="3.4.21.61"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0004252 serine-type
endopeptidase activity [PMID:2646633]"
/experiment="EXISTENCE:direct assay:GO:0005802 trans-Golgi
network [PMID:8308064]"
/experiment="EXISTENCE:mutant phenotype:GO:0007323 peptide
pheromone maturation [PMID:6430565]"
/note="Kexin, a subtilisin-like protease (proprotein
convertase); a calcium-dependent serine protease involved
in the activation of proproteins of the secretory pathway"
/codon_start=1
/product="kexin KEX2"
/protein_id="NP_014161.1"
/db_xref="GeneID:855483"
/db_xref="SGD:S000005182"
/translation="MKVRKYITLCFWWAFSTSALVSSQQIPLKDHTSRQYFAVESNET
LSRLEEMHPNWKYEHDVRGLPNHYVFSKELLKLGKRSSLEELQGDNNDHILSVHDLFP
RNDLFKRLPVPAPPMDSSLLPVKEAEDKLSINDPLFERQWHLVNPSFPGSDINVLDLW
YNNITGAGVVAAIVDDGLDYENEDLKDNFCAEGSWDFNDNTNLPKPRLSDDYHGTRCA
GEIAAKKGNNFCGVGVGYNAKISGIRILSGDITTEDEAASLIYGLDVNDIYSCSWGPA
DDGRHLQGPSDLVKKALVKGVTEGRDSKGAIYVFASGNGGTRGDNCNYDGYTNSIYSI
TIGAIDHKDLHPPYSEGCSAVMAVTYSSGSGEYIHSSDINGRCSNSHGGTSAAAPLAA
GVYTLLLEANPNLTWRDVQYLSILSAVGLEKNADGDWRDSAMGKKYSHRYGFGKIDAH
KLIEMSKTWENVNAQTWFYLPTLYVSQSTNSTEETLESVITISEKSLQDANFKRIEHV
TVTVDIDTEIRGTTTVDLISPAGIISNLGVVRPRDVSSEGFKDWTFMSVAHWGENGVG
DWKIKVKTTENGHRIDFHSWRLKLFGESIDSSKTETFVFGNDKEEVEPAATESTVSQY
SASSTSISISATSTSSISIGVETSAIPQTTTASTDPDSDPNTPKKLSSPRQAMHYFLT
IFLIGATFLVLYFMFFMKSRRRIRRSRAETYEFDIIDTDSEYDSTLDNGTSGITEPEE
VEDFDFDLSDEDHLASLSSSENGDAEHTIDSVLTNENPFSDPIKQKFPNDANAESASN
KLQELQPDVPPSSGRS"
gene <205188..>206567
/gene="YTP1"
/locus_tag="YNL237W"
/db_xref="GeneID:855484"
mRNA <205188..>206567
/gene="YTP1"
/locus_tag="YNL237W"
/product="Ytp1p"
/transcript_id="NM_001183075.1"
/db_xref="GeneID:855484"
CDS 205188..206567
/gene="YTP1"
/locus_tag="YNL237W"
/note="Probable type-III integral membrane hypothetical
protein; has regions of similarity to mitochondrial
electron transport proteins"
/codon_start=1
/product="Ytp1p"
/protein_id="NP_014162.1"
/db_xref="GeneID:855484"
/db_xref="SGD:S000005181"
/translation="MTAANKNIVFGFSRSISAILLICFFFEKVCGDMEHDMGMDDTSG
YTRPEIVQAGSKSFHWLCTLGFLLLLPSVVTCLSFAGRIYSATLLQCTCAVYAFLEAA
VLRFQDNDGVENRTSRGTAWFLVGLTWITLFFGGLAGGTGFLVKSKRLQTFISNAGEK
RLSYIHRGLSFLTVLTGWVKVCLAPVALFGFCREAHTGQCIAHGIMGSAFVLYGFIYV
LVLVIPWIRSAQTSYSQDYVDSWVMCIWGVVNTFTEHRWGREGWSVHDYQHTFMGIIW
WTGGILGIFLSRNGRRTFVPSLIIIFTGWAMSEHAQHLIISTKVHNMFGLVLMCGGAL
RIIEISFLLRDKRTLDKIHSFQYLAPFCLVCSGLLFMGANEEQLILVLRLGGDHSAYV
LIIVSGAFLVYFWMIACLEFYLYLLEKGKQGFLPKSYELEEENNNVSFELDNISNEDV
DEDTTPFNV"
gene <206930..>209854
/gene="SIN4"
/locus_tag="YNL236W"
/gene_synonym="BEL2; GAL22; MED16; RYE1; SDI3; SSF5; SSN4;
SSX3; TSF3"
/db_xref="GeneID:855485"
mRNA <206930..>209854
/gene="SIN4"
/locus_tag="YNL236W"
/gene_synonym="BEL2; GAL22; MED16; RYE1; SDI3; SSF5; SSN4;
SSX3; TSF3"
/product="Sin4p"
/transcript_id="NM_001183074.1"
/db_xref="GeneID:855485"
CDS 206930..209854
/gene="SIN4"
/locus_tag="YNL236W"
/gene_synonym="BEL2; GAL22; MED16; RYE1; SDI3; SSF5; SSN4;
SSX3; TSF3"
/experiment="EXISTENCE:direct assay:GO:0016592 mediator
complex [PMID:9891034]"
/experiment="EXISTENCE:direct assay:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:20308326]"
/experiment="EXISTENCE:direct assay:GO:0070847 core
mediator complex [PMID:9891034]"
/experiment="EXISTENCE:genetic interaction:GO:0034605
cellular response to heat [PMID:23447536]"
/experiment="EXISTENCE:genetic interaction:GO:0070202
regulation of establishment of protein localization to
chromosome [PMID:23447536]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:16452140]"
/experiment="EXISTENCE:mutant phenotype:GO:0034605
cellular response to heat [PMID:23447536]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:12558798]"
/experiment="EXISTENCE:mutant phenotype:GO:0051123 RNA
polymerase II preinitiation complex assembly
[PMID:12482986]"
/experiment="EXISTENCE:mutant phenotype:GO:0070202
regulation of establishment of protein localization to
chromosome [PMID:23447536]"
/note="Subunit of the RNA polymerase II mediator complex;
associates with core polymerase subunits to form the RNA
polymerase II holoenzyme; contributes to both positive and
negative transcriptional regulation; dispensible for basal
transcription"
/codon_start=1
/product="Sin4p"
/protein_id="NP_014163.1"
/db_xref="GeneID:855485"
/db_xref="SGD:S000005180"
/translation="MMLGEHLMSWSKTGIIAYSDSQSSNANICLTFLESINGINWRFH
TPQKYVLHPQLHEVQYQESSSTLSTHSTTTSVNGSTTAGVGSTPNFGGNSNKSPPQFF
YNISSIHWNNWFSLPGDMLAVCDELGNMTMLITGQRPDRATTYEKLTMVFQDNVYKIY
NHVMPLKPVDKLKPMNIERKQTRKEYNTSILEFRWLTSSKSVIVSQFCAFDSSSNTYR
SRAQQVPPYGVYHPPFIKYACLAIRKNGQIDFWYQFSNSKDHKKITLQLLDTSNQRFK
DLQWLEFARITPMNDDQCMLITTYSKLSKNISFYKLHVNWNLNATKPNVLNDPSLKIQ
FILSTTLDPTDDEGHVLKLENLHVVSKSSIEKDPSPEILVLYNVCDTSKSLVKRYRLA
PTQLSAEYLVILKPDLNIDRNNSTNQIFQSRRYNLRRHSDIVLDKKVTLITSEMFDAF
VSFYFEDGTIESYNQNDWKLETERLISQSQLGKFKNIIASPLSAGFNYGKLPLPPSVE
WMKVSPSMCGVIVKQYNKKWPQFYAAVQKNYADPEKDSINATALAFGYVKSLHKQISA
EDLTIAAKTHILRISFLDRKRAKEFITTLLKSLYSFFNISPDAPKEIMDKIITSRPLQ
KIMLLQLELGSCFSQENIEEMARVILYLKNVLFAFNGVARNFHFAIEQISNNSNQQQN
PKLFQTIFSKQDLIHSLIPVAKWFVKFITYLTQEILILINDPTNKEYTLVHGIFGAKM
SRTLILSILNEIKKVTQIVAKFPETSYPILNESSTFLKLVLSESPVDFEKFETFLVDV
NNKFIALCEQQPSQEREFSLLVKAEIPPEYAKVGDFLLQYANNAVISHANAAAVYFAD
TSGLKISNSEFFNPEIFHLLQPLEEGLIIDTDKLPIKNRTSKSFSKLLYDDVTCDKLS
VSEISDGKLKRCSRCGSVTRAGNIISSDKTIVPTSIQTKRWPTMYTRLCICSGMLFEM
DG"
gene <210233..>211513
/locus_tag="YNL234W"
/db_xref="GeneID:855487"
mRNA <210233..>211513
/locus_tag="YNL234W"
/product="uncharacterized protein"
/transcript_id="NM_001183072.1"
/db_xref="GeneID:855487"
CDS 210233..211513
/locus_tag="YNL234W"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0020037 heme
binding [PMID:9988749]"
/note="hypothetical protein with similarity to globins;
has a functional heme-binding domain; mutant has
aneuploidy tolerance; transcription induced by stress
conditions; may be involved in glucose signaling or
metabolism; regulated by Rgt1"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014165.1"
/db_xref="GeneID:855487"
/db_xref="SGD:S000005178"
/translation="MTGEKILHSQLLTNSDMSSGNVHHTKPMMYNVTLPSYNSSSIGP
VDNLKINERPGSHDHSMRSEMSSKNSGSDFMPQSISRSEGSVYQVKIDRGDSPNTEGF
DFKVNARDLLLLRMSWDILLREYLTPKELKVFQALLYSNKHITSTERPYLNTAPDGMI
SKTIDPTARPRKTKQRDNDNKVDTALFCSQFYDNLIAMDPLLEEYFPSLKHQAVSFCK
VLDSAIDNLENVHVLDDYIVKLGKRHSRILGIKTVGFEVMGKAFMTTLQDRFGSFLTL
ELKNLWGQLYSYLANCMITAGKDPMEKIQPDFSYNGDSVVLNFSIPKLAMHDISTVNK
LQMVKTKNATIPHNITQVPTNKIPTEILLDNSSTPIKSDRESTPPISPKGSGSTKPSI
GSSTVVESNTKKNNYDEKIHLLQKTAQQKNCSIM"
gene <211922..>214600
/gene="BNI4"
/locus_tag="YNL233W"
/db_xref="GeneID:855488"
mRNA <211922..>214600
/gene="BNI4"
/locus_tag="YNL233W"
/product="Bni4p"
/transcript_id="NM_001183071.1"
/db_xref="GeneID:855488"
CDS 211922..214600
/gene="BNI4"
/locus_tag="YNL233W"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:15470103|PMID:15901837]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:15282802]"
/experiment="EXISTENCE:direct assay:GO:0005940 septin ring
[PMID:9314530|PMID:15901837]"
/experiment="EXISTENCE:direct assay:GO:0032174 cellular
bud neck septin collar [PMID:15901837]"
/experiment="EXISTENCE:mutant phenotype:GO:0000917
division septum assembly [PMID:15470103]"
/experiment="EXISTENCE:mutant phenotype:GO:0006031 chitin
biosynthetic process [PMID:15470103]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:9314530|PMID:12529424]"
/note="Targeting subunit for Glc7p protein phosphatase;
localized to the bud neck, required for localization of
chitin synthase III to the bud neck via interaction with
the chitin synthase III regulatory subunit Skt5p;
phosphorylation by Slt2p and Kss1p involved in regulating
Bni4p in septum assembly"
/codon_start=1
/product="Bni4p"
/protein_id="NP_014166.1"
/db_xref="GeneID:855488"
/db_xref="SGD:S000005177"
/translation="MSDSISDSKSSELLNSTFYSSTSINTLDHARTFRNSLILKEISD
QSLNSSIKPCESVLDRDVESSVLQRSFGESNARDSEVQTVNMTTSPSLSALADILNER
SKYADQKTRKAQNIESSIIEEEEEAEEQNNSINYHEDITGSRLSVREEANENLAMTSP
NLIDIDGSNSIQVAPLSLPSFEEPDFLSTPRVKPDSQGPRSKVSTRRTILERDNNLPV
KREENTIINSETESTTHSAPFLKEDPKPSPPSSKLYNPKVRLNKAEARKYTDSSAQRT
TSAGSVLEDTSMHKKKKSIFSFLKKKEPKPVIGNNSVTNEKNKMSSSSTFSMNIQTSL
KTPEKLKKKSHSSSSIFNSFLKGKIETSDSPRKEPMRQKKRTPKSKDKKQDTEQIIDA
ASVLSTESPLLRKNHDDTPVKIDHVTRSIDQRKPTPLNMDLILGGDKQINTPLQEHVR
EDDDAKNDLQLPTKDNFLSLDYEAPSPAFSKHDTGEVLFPKFLDNHEVDSIVSLERTR
STKSNKRSSMNSQRRSLTDTLSIKAQSEGMFITEASSVVLSTPDLTKSPASSILKNGR
FEYSDNFSREHSYEGTTNEDFLDIKDDSGPLKKDDIFLESIEQKFDQLVMASDEEKTE
VERDVPKPREEPLKKDSERQSVFADDDNELISDIMEFASFINFGDDDLNLDLDLGDTT
ASYATETPEPVGNDEVNRSGTFDTRNNKEDSYKERETQSYSAAGATTYGDERQGQLHT
FEQDGSEINDNEFENEDFNKHIEQPIEVTPRNNAYLPEFEPNRPVSMSFKGLKAPRMN
TSFIDSMTPDSPVKSDLTSLGEVYVNSNNDQGVRFSSQIILYDTYGEFEYDRHPEIST
CNQLTPQLAQMIKLELNELKSAMEVHDDSRCYTHFY"
gene <214923..>215801
/gene="CSL4"
/locus_tag="YNL232W"
/gene_synonym="SKI4"
/db_xref="GeneID:855489"
mRNA <214923..>215801
/gene="CSL4"
/locus_tag="YNL232W"
/gene_synonym="SKI4"
/product="exosome non-catalytic core subunit CSL4"
/transcript_id="NM_001183070.1"
/db_xref="GeneID:855489"
CDS 214923..215801
/gene="CSL4"
/locus_tag="YNL232W"
/gene_synonym="SKI4"
/experiment="EXISTENCE:direct assay:GO:0000176 nuclear
exosome (RNase complex) [PMID:10465791|PMID:19046973]"
/experiment="EXISTENCE:direct assay:GO:0000177 cytoplasmic
exosome (RNase complex) [PMID:10465791|PMID:19046973]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11027292]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11027292]"
/experiment="EXISTENCE:direct assay:GO:0071038
TRAMP-dependent tRNA surveillance pathway
[PMID:17643380|PMID:15828860|PMID:15935758]"
/experiment="EXISTENCE:genetic interaction:GO:0000956
nuclear-transcribed mRNA catabolic process
[PMID:11027292|PMID:19060898]"
/experiment="EXISTENCE:mutant phenotype:GO:0000467
exonucleolytic trimming to generate mature 3'-end of 5.8S
rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S
rRNA, LSU-rRNA) [PMID:10465791|PMID:10508172]"
/experiment="EXISTENCE:mutant phenotype:GO:0000956
nuclear-transcribed mRNA catabolic process
[PMID:11027292|PMID:19060898]"
/experiment="EXISTENCE:mutant phenotype:GO:0006397 mRNA
processing [PMID:27385342]"
/experiment="EXISTENCE:mutant phenotype:GO:0070481
nuclear-transcribed mRNA catabolic process, non-stop decay
[PMID:11910110]"
/experiment="EXISTENCE:mutant phenotype:GO:0071035 nuclear
polyadenylation-dependent rRNA catabolic process
[PMID:10465791]"
/note="Exosome non-catalytic core component; involved in
3'-5' RNA processing and degradation in both the nucleus
and the cytoplasm; predicted to contain an S1 RNA binding
domain; human homolog EXOSC1 partially complements yeast
csl4 null mutant, and can complement inviability of strain
in which expression of CSL4 is repressed"
/codon_start=1
/product="exosome non-catalytic core subunit CSL4"
/protein_id="NP_014167.1"
/db_xref="GeneID:855489"
/db_xref="SGD:S000005176"
/translation="MACNFQFPEIAYPGKLICPQYGTENKDGEDIIFNYVPGPGTKLI
QYEHNGRTLEAITATLVGTVRCEEEKKTDQEEEREGTDQSTEEEKSVDASPNDVTRRT
VKNILVSVLPGTEKGRKTNKYANNDFANNLPKEGDIVLTRVTRLSLQRANVEILAVED
KPSPIDSGIGSNGSGIVAAGGGSGAATFSVSQASSDLGETFRGIIRSQDVRSTDRDRV
KVIECFKPGDIVRAQVLSLGDGTNYYLTTARNDLGVVFARAANGAGGLMYATDWQMMT
SPVTGATEKRKCAKPF"
gene complement(<215987..>217042)
/gene="PDR16"
/locus_tag="YNL231C"
/gene_synonym="SFH3"
/db_xref="GeneID:855490"
mRNA complement(<215987..>217042)
/gene="PDR16"
/locus_tag="YNL231C"
/gene_synonym="SFH3"
/product="phosphatidylinositol transporter"
/transcript_id="NM_001183069.1"
/db_xref="GeneID:855490"
CDS complement(215987..217042)
/gene="PDR16"
/locus_tag="YNL231C"
/gene_synonym="SFH3"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005811 lipid
droplet [PMID:24403601|PMID:12869188]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836]"
/experiment="EXISTENCE:direct assay:GO:0008526
phosphatidylinositol transfer activity [PMID:10848624]"
/experiment="EXISTENCE:direct assay:GO:0015914
phospholipid transport [PMID:10848624]"
/experiment="EXISTENCE:direct assay:GO:0032934 sterol
binding [PMID:36482167|PMID:31690622]"
/experiment="EXISTENCE:direct assay:GO:0071561
nucleus-vacuole junction [PMID:29187527]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:12869188]"
/experiment="EXISTENCE:genetic interaction:GO:0008526
phosphatidylinositol transfer activity [PMID:9890948]"
/experiment="EXISTENCE:genetic interaction:GO:0008654
phospholipid biosynthetic process [PMID:9890948]"
/experiment="EXISTENCE:genetic interaction:GO:0009410
response to xenobiotic stimulus [PMID:9890948]"
/experiment="EXISTENCE:genetic interaction:GO:0015914
phospholipid transport [PMID:9890948]"
/experiment="EXISTENCE:genetic interaction:GO:0016126
sterol biosynthetic process [PMID:9890948]"
/experiment="EXISTENCE:mutant phenotype:GO:0008526
phosphatidylinositol transfer activity [PMID:9890948]"
/experiment="EXISTENCE:mutant phenotype:GO:0015914
phospholipid transport [PMID:9890948]"
/experiment="EXISTENCE:mutant phenotype:GO:0043942
negative regulation of sexual sporulation resulting in
formation of a cellular spore [PMID:24403601]"
/note="Phosphatidylinositol transfer protein (PITP);
controlled by the multiple drug resistance regulator
Pdr1p; localizes to lipid particles and microsomes;
controls levels of various lipids, may regulate lipid
synthesis; homologous to Pdr17p; protein abundance
increases in response to DNA replication stress"
/codon_start=1
/product="phosphatidylinositol transporter"
/protein_id="NP_014168.1"
/db_xref="GeneID:855490"
/db_xref="SGD:S000005175"
/translation="MFKRFSKKKEAPEDPKNLINIDKPIKELPASIAIPKEKPLTGEQ
QKMYDEVLKHFSNPDLKVYTSEKNKSEDDLKPLEEEEKAWLTRECFLRYLRATKWVLK
DCIDRITMTLAWRREFGISHLGEEHGDKITADLVAVENESGKQVILGYENDARPILYL
KPGRQNTKTSHRQVQHLVFMLERVIDFMPAGQDSLALLIDFKDYPDVPKVPGNSKIPP
IGVGKEVLHILQTHYPERLGKALLTNIPWLAWTFLKLIHPFIDPLTREKLVFDEPFVK
YVPKNELDSLYGGDLKFKYNHDVYWPALVETAREKRDHYFKRFQSFGGIVGLSEVDLR
GTHEKLLYPVKSESSTV"
gene complement(<217523..>218662)
/gene="ELA1"
/locus_tag="YNL230C"
/db_xref="GeneID:855491"
mRNA complement(<217523..>218662)
/gene="ELA1"
/locus_tag="YNL230C"
/product="elongin A"
/transcript_id="NM_001183068.1"
/db_xref="GeneID:855491"
CDS complement(217523..218662)
/gene="ELA1"
/locus_tag="YNL230C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:mutant phenotype:GO:0004842
ubiquitin-protein transferase activity [PMID:17296727]"
/experiment="EXISTENCE:mutant phenotype:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:17296727]"
/experiment="EXISTENCE:mutant phenotype:GO:0031463
Cul3-RING ubiquitin ligase complex [PMID:17296727]"
/experiment="EXISTENCE:physical interaction:GO:0070449
elongin complex [PMID:10753924|PMID:10430890]"
/note="Elongin A; F-box protein that forms a heterodimer
with Elc1p and is required for ubiquitin-dependent
degradation of the RNA Polymerase II subunit Rpo21p;
subunit of the Elongin-Cullin-Socs (ECS) ligase complex"
/codon_start=1
/product="elongin A"
/protein_id="NP_014169.1"
/db_xref="GeneID:855491"
/db_xref="SGD:S000005174"
/translation="MKSLQTLCEISLMRNHSNIQSVSNVPYHLLKRILQKVKIPQLLK
LEKSNVLLIFDDDELWLEFLRQDFPTNVHEQFVSKRDIICKYYFDFVKENDIELYHSN
QDLLKSCVRQSVVKDIRNNKYRIPYRMLYSKYQQEVEKKQEESAERLRLEMQKLQQER
EKKQTIVVDHTVYFKKRNTKKTTRLHNEPHSQLYMKSLKDHESRLKHFKDGGFNIAKR
HAQRVAFGGQAGGQSSSPKKGPLSIKPEPVKVNRQMDNVTAEKKDVTQPITPVKKRRS
ESPSIFLNRKKPALFRPTPKTNAAGSRPHTTAITNDHRTTSHPYPHKDVVTSISSVTA
NPVTKGHKKKKSGIFVRNAGSDGDSFPHVTATGPTTRPYIYEPRK"
gene complement(<219137..>220201)
/gene="URE2"
/locus_tag="YNL229C"
/db_xref="GeneID:855492"
mRNA complement(<219137..>220201)
/gene="URE2"
/locus_tag="YNL229C"
/product="glutathione peroxidase"
/transcript_id="NM_001183067.1"
/db_xref="GeneID:855492"
CDS complement(219137..220201)
/gene="URE2"
/locus_tag="YNL229C"
/EC_number="1.11.1.9"
/experiment="EXISTENCE:direct assay:GO:0004602 glutathione
peroxidase activity [PMID:15371425]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:10748041]"
/experiment="EXISTENCE:direct assay:GO:0051219
phosphoprotein binding [PMID:10604478]"
/experiment="EXISTENCE:genetic interaction:GO:0006808
regulation of nitrogen utilization [PMID:8755910]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:10748041|PMID:10799523]"
/experiment="EXISTENCE:mutant phenotype:GO:0006808
regulation of nitrogen utilization [PMID:8002570]"
/experiment="EXISTENCE:mutant phenotype:GO:0032447 protein
urmylation [PMID:14551258]"
/experiment="EXISTENCE:physical interaction:GO:0006808
regulation of nitrogen utilization [PMID:8755910]"
/note="Nitrogen catabolite repression transcriptional
regulator; inhibits GLN3 transcription in good nitrogen
source; role in sequestering Gln3p and Gat1p to the
cytoplasm; has glutathione peroxidase activity and can
mutate to acquire GST activity; catabolite repression
normally induced by limiting nitrogen does not occur if
Ure2p has polymerized to form the [URE3] prion"
/codon_start=1
/product="glutathione peroxidase"
/protein_id="NP_014170.1"
/db_xref="GeneID:855492"
/db_xref="SGD:S000005173"
/translation="MMNNNGNQVSNLSNALRQVNIGNRNSNTTTDQSNINFEFSTGVN
NNNNNNSSSNNNNVQNNNSGRNGSQNNDNENNIKNTLEQHRQQQQAFSDMSHVEYSRI
TKFFQEQPLEGYTLFSHRSAPNGFKVAIVLSELGFHYNTIFLDFNLGEHRAPEFVSVN
PNARVPALIDHGMDNLSIWESGAILLHLVNKYYKETGNPLLWSDDLADQSQINAWLFF
QTSGHAPMIGQALHFRYFHSQKIASAVERYTDEVRRVYGVVEMALAERREALVMELDT
ENAAAYSAGTTPMSQSRFFDYPVWLVGDKLTIADLAFVPWNNVVDRIGINIKIEFPEV
YKWTKHMMRRPAVIKALRGE"
gene complement(<220659..>222431)
/gene="JJJ1"
/locus_tag="YNL227C"
/db_xref="GeneID:855495"
mRNA complement(<220659..>222431)
/gene="JJJ1"
/locus_tag="YNL227C"
/product="Jjj1p"
/transcript_id="NM_001183065.1"
/db_xref="GeneID:855495"
CDS complement(220659..222431)
/gene="JJJ1"
/locus_tag="YNL227C"
/experiment="EXISTENCE:direct assay:GO:0001671 ATPase
activator activity [PMID:17242366]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:20368619|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:17242366|PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0000055
ribosomal large subunit export from nucleus
[PMID:20368619]"
/experiment="EXISTENCE:mutant phenotype:GO:0005730
nucleolus [PMID:20368619]"
/experiment="EXISTENCE:mutant phenotype:GO:0006364 rRNA
processing [PMID:20368619]"
/experiment="EXISTENCE:mutant phenotype:GO:0006897
endocytosis [PMID:11378903]"
/experiment="EXISTENCE:mutant phenotype:GO:0008361
regulation of cell size [PMID:12089449]"
/experiment="EXISTENCE:mutant phenotype:GO:0042273
ribosomal large subunit biogenesis [PMID:17242366]"
/note="Co-chaperone that stimulates the ATPase activity of
Ssa1p; required for a late step of ribosome biogenesis;
associated with the cytosolic large ribosomal subunit;
contains a J-domain; mutation causes defects in
fluid-phase endocytosis"
/codon_start=1
/product="Jjj1p"
/protein_id="NP_014172.1"
/db_xref="GeneID:855495"
/db_xref="SGD:S000005171"
/translation="MKTCYYELLGVETHASDLELKKAYRKKALQYHPDKNPDNVEEAT
QKFAVIRAAYEVLSDPQERAWYDSHKEQILNDTPPSTDDYYDYEVDATVTGVTTDELL
LFFNSALYTKIDNSAAGIYQIAGKIFAKLAKDEILSGKRLGKFSEYQDDVFEQDINSI
GYLKACDNFINKTDKLLYPLFGYSPTDYEYLKHFYKTWSAFNTLKSFSWKDEYMYSKN
YDRRTKREVNRRNEKARQQARNEYNKTVKRFVVFIKKLDKRMKEGAKIAEEQRKLKEQ
QRKNELNNRRKFGNDNNDEEKFHLQSWQTVKEENWDELEKVYDNFGEFENSKNDKEGE
VLIYECFICNKTFKSEKQLKNHINTKLHKKNMEEIRKEMEEENITLGLDNLSDLEKFD
SADESVKEKEDIDLQALQAELAEIERKLAESSSEDESEDDNLNIEMDIEVEDVSSDEN
VHVNTKNKKKRKKKKKAKVDTETEESESFDDTKDKRSNELDDLLASLGDKGLQTDDDE
DWSTKAKKKKGKQPKKNSKSTKSTPSLSTLPSSMSPTSAIEVCTTCGESFDSRNKLFN
HVKIAGHAAVKNVVKRKKVKTKRI"
gene complement(<222724..>224469)
/gene="CNM67"
/locus_tag="YNL225C"
/db_xref="GeneID:855496"
mRNA complement(<222724..>224469)
/gene="CNM67"
/locus_tag="YNL225C"
/product="Cnm67p"
/transcript_id="NM_001183063.1"
/db_xref="GeneID:855496"
CDS complement(222724..224469)
/gene="CNM67"
/locus_tag="YNL225C"
/experiment="EXISTENCE:direct assay:GO:0005816 spindle
pole body [PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0005824 outer
plaque of spindle pole body [PMID:11514632]"
/experiment="EXISTENCE:direct assay:GO:0061499 outer
plaque of mitotic spindle pole body [PMID:9585415]"
/experiment="EXISTENCE:mutant phenotype:GO:0005200
structural constituent of cytoskeleton [PMID:11514632]"
/experiment="EXISTENCE:mutant phenotype:GO:0007052 mitotic
spindle organization [PMID:9571234|PMID:10749924]"
/experiment="EXISTENCE:mutant phenotype:GO:0051300 spindle
pole body organization [PMID:9571234]"
/note="Component of the spindle pole body outer plaque;
required for spindle orientation and mitotic nuclear
migration; CNM67 has a paralog, ADY3, that arose from the
whole genome duplication"
/codon_start=1
/product="Cnm67p"
/protein_id="NP_014174.1"
/db_xref="GeneID:855496"
/db_xref="SGD:S000005169"
/translation="MTDFDLMNFPFHERLDSPVSENGEIKDGEPIPQNWLNENHVGKS
ILPLFVNPEDVINCNFSNARDSYEENKSPSMDQMNYARNTSYQESPGLQERPKNEKDK
SPIGTDVHKKDVPNFIHSTPRENSSKHFTRANEQASAQPTDEHTSPDISIEDCNGAKI
FLQNSLSKEDFRMLENVILGYQKKVIELGRDNLRQEERANSLQKELEAATKSNDKTLD
NKKKIEEQTVLIENLTKDLSLNKEMLEKANDTIQTKHTALLSLTDSLRKAELFEIPIG
ILFFDLYDSEENSSKLDHILQEKYPNIKGFLCASQQEELSRISQRFKNAKAEAEDLRN
ELENKKIEIQTMREKNNTLIGTNKTLSKQNKILCDKFDKLTIDEKEILKGCNEEIKIK
LERLNERLGSWEKSKEKYETSLKDKEKMLADAEKKTNTLSKELDNLRSRFGNLEGNTS
ERITIKNILQSRPDISAEECNFLMVEQIDSANLTTLQNTVKEIVLAVGIPYPKLRRKI
PLLAIKLKYENIMLSNFAQRLHRQVYSQEMNLKKFTDQAYYDFMSTRRMDSIDHHLER
CLDHLYDHILEKMVK"
gene complement(<224796..>227099)
/gene="SQS1"
/locus_tag="YNL224C"
/gene_synonym="PFA1"
/db_xref="GeneID:855497"
mRNA complement(<224796..>227099)
/gene="SQS1"
/locus_tag="YNL224C"
/gene_synonym="PFA1"
/product="Sqs1p"
/transcript_id="NM_001183062.1"
/db_xref="GeneID:855497"
CDS complement(224796..227099)
/gene="SQS1"
/locus_tag="YNL224C"
/gene_synonym="PFA1"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008047 enzyme
activator activity [PMID:19927118]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:19927118]"
/experiment="EXISTENCE:direct assay:GO:0030687
preribosome, large subunit precursor [PMID:19927118]"
/experiment="EXISTENCE:direct assay:GO:0030688
preribosome, small subunit precursor
[PMID:19927118|PMID:16227579]"
/experiment="EXISTENCE:genetic interaction:GO:0000398 mRNA
splicing, via spliceosome [PMID:16945917]"
/experiment="EXISTENCE:genetic interaction:GO:0006364 rRNA
processing [PMID:19927118]"
/experiment="EXISTENCE:genetic interaction:GO:0030490
maturation of SSU-rRNA [PMID:19801658|PMID:19927118]"
/experiment="EXISTENCE:mutant phenotype:GO:0008047 enzyme
activator activity [PMID:19927118]"
/note="Protein that stimulates the ATPase and helicase
activities of Prp43p; acts with Prp43p to stimulate 18s
rRNA maturation by Nob1p; overexpression antagonizes the
suppression of splicing defects by spp382 mutants;
component of pre-ribosomal particles; relocalizes from
nucleus to nucleolus upon DNA replication stress"
/codon_start=1
/product="Sqs1p"
/protein_id="NP_014175.1"
/db_xref="GeneID:855497"
/db_xref="SGD:S000005168"
/translation="MAKRHSHYQGSRRRHARGSNSKKAGRGNAKGIQGRKIKKKPTPT
NSWHNSSIPLGEGDLDDVGADFNPGRAFISPKTIEDYYFGRDAKSRSMKMGGLRPGNR
YDSSTDLQAGRAAFRKRPMQFVKAKEVYDPSHNMIQKLRAKNETKNSEEIVEREADVF
EEPGKMTSDVEYINNEDSENEDDDSQNSPSTDHSLSSNESKVEDGDLFFVDEEAQQSP
DLTKIKRVCIEEIARPREVAIEFDPILTIGKVELSVSEGNESKEISVDVPNKGNKTYH
PFAGYISNVLHGMHTSDSDNDELDYEIETENNSEPLYESSASSEVDQGFNYVGQRHNS
RADNNLLPSPSPQLTEDIKCLSINGTKTFEGNNDNLPSPASEELEFGFKEEDFVINTN
DIVVSNIRMGGVDNSYYLRCYRLLGDYDFHWIDQDLLTDFVVDELGLPEDRLPAYLNF
IKNSLIPKIEPAEPTYSDIPISDSSDEGDSYEGDSYEDDEDMASSVVHSDIEEGLDDL
IAYTLKHDTERFKTFETKSLETKGKGKKKKLLIDDALALDTETLETLQSKFSKRIETK
AKKRKAKEDFIDQENRNSNDMLKKYPYGLHIQNIKDEFESFLSRNNDRLTFPPLDPHG
NKTVMKIAKHYNMKSSKIGKANHTSVVVEKIKKTKWSSPNYSLIDQLMRQRPVFMRID
IRRPREEQAAFERTKTIRGKFHVKEGEIVGQNAPEIGNENIGRRMLEKLGWKSGEGLG
IQGNKGISEPIFAKIKKNRSGLRHSES"
gene <227370..>228854
/gene="ATG4"
/locus_tag="YNL223W"
/gene_synonym="APG4; AUT2"
/db_xref="GeneID:855498"
mRNA <227370..>228854
/gene="ATG4"
/locus_tag="YNL223W"
/gene_synonym="APG4; AUT2"
/product="cysteine protease ATG4"
/transcript_id="NM_001183061.1"
/db_xref="GeneID:855498"
CDS 227370..228854
/gene="ATG4"
/locus_tag="YNL223W"
/gene_synonym="APG4; AUT2"
/experiment="EXISTENCE:direct assay:GO:0004197
cysteine-type endopeptidase activity [PMID:11038174]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22240591]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22240591]"
/experiment="EXISTENCE:direct assay:GO:0019786
protein-phosphatidylethanolamide deconjugating activity
[PMID:11038174]"
/experiment="EXISTENCE:direct assay:GO:0051697 protein
delipidation [PMID:17632063]"
/experiment="EXISTENCE:mutant phenotype:GO:0000422
autophagy of mitochondrion [PMID:19793921]"
/experiment="EXISTENCE:mutant phenotype:GO:0004197
cysteine-type endopeptidase activity [PMID:11038174]"
/experiment="EXISTENCE:mutant phenotype:GO:0006501
C-terminal protein lipidation [PMID:11038174]"
/experiment="EXISTENCE:mutant phenotype:GO:0019786
protein-phosphatidylethanolamide deconjugating activity
[PMID:11038174]"
/experiment="EXISTENCE:mutant phenotype:GO:0032258
cytoplasm to vacuole targeting by the Cvt pathway
[PMID:11149920]"
/experiment="EXISTENCE:mutant phenotype:GO:0034727
piecemeal microautophagy of the nucleus [PMID:18701704]"
/experiment="EXISTENCE:mutant phenotype:GO:0044804
nucleophagy [PMID:22768199]"
/note="Conserved cysteine protease required for autophagy;
functions in both early and late stages of autophagosome
formation; primes Atg8p by removing the C-terminal
arginine residue of nascent Atg8p in preparation for
conjugation with phosphatidylethanolamide (PE), and also
cleaves the PE modified, membrane-bound form to reverse
conjugation; regulates the membrane-binding state of
Atg8p, that is required for autophagosome formation and
autophagy, as well as the cytoplasm-to-vacuole targeting
pathway"
/codon_start=1
/product="cysteine protease ATG4"
/protein_id="NP_014176.2"
/db_xref="GeneID:855498"
/db_xref="SGD:S000005167"
/translation="MQRWLQLWKMDLVQKVSHGVFEGSSEEPAALMNHDYIVLGEVYP
ERDEESGAEQCEQDCRYRGEAVSDGFLSSLFGREISSYTKEFLLDVQSRVNFTYRTRF
VPIARAPDGPSPLSLNLLVRTNPISTIEDYIANPDCFNTDIGWGCMIRTGQSLLGNAL
QILHLGRDFRVNGNESLERESKFVNWFNDTPEAPFSLHNFVSAGTELSDKRPGEWFGP
AATARSIQSLIYGFPECGIDDCIVSVSSGDIYENEVEKVFAENPNSRILFLLGVKLGI
NAVNESYRESICGILSSTQSVGIAGGRPSSSLYFFGYQGNEFLHFDPHIPQPAVEDSF
VESCHTSKFGKLQLSEMDPSMLIGILIKGEKDWQQWKLEVAESAIINVLAKRMDDFDV
SCSMDDVESVSSNSMKKDASNNENLGVLEGDYVDIGAIFPHTTNTEDVDEYDCFQDIH
CKKQKIVVMGNTHTVNANLTDYEVEGVLVEKETVGIHSPIDEKC"
gene <229094..>229714
/gene="SSU72"
/locus_tag="YNL222W"
/db_xref="GeneID:855499"
mRNA <229094..>229714
/gene="SSU72"
/locus_tag="YNL222W"
/product="RNA polymerase II subunit A C-terminal domain
phosphatase"
/transcript_id="NM_001183060.1"
/db_xref="GeneID:855499"
CDS 229094..229714
/gene="SSU72"
/locus_tag="YNL222W"
/EC_number="3.1.3.16"
/experiment="EXISTENCE:direct assay:GO:0004722 protein
serine/threonine phosphatase activity [PMID:22235117]"
/experiment="EXISTENCE:direct assay:GO:0004725 protein
tyrosine phosphatase activity [PMID:12660165]"
/experiment="EXISTENCE:direct assay:GO:0005847 mRNA
cleavage and polyadenylation specificity factor complex
[PMID:12819204]"
/experiment="EXISTENCE:direct assay:GO:0008420 RNA
polymerase II CTD heptapeptide repeat phosphatase activity
[PMID:15125841|PMID:22235117]"
/experiment="EXISTENCE:genetic interaction:GO:0001174
transcriptional start site selection at RNA polymerase II
promoter [PMID:8657130]"
/experiment="EXISTENCE:genetic interaction:GO:0004721
phosphoprotein phosphatase activity [PMID:12660165]"
/experiment="EXISTENCE:genetic interaction:GO:0005634
nucleus [PMID:11046131]"
/experiment="EXISTENCE:genetic interaction:GO:0006367
transcription initiation at RNA polymerase II promoter
[PMID:11046131]"
/experiment="EXISTENCE:genetic interaction:GO:0006368
transcription elongation by RNA polymerase II
[PMID:17101794]"
/experiment="EXISTENCE:mutant phenotype:GO:0001174
transcriptional start site selection at RNA polymerase II
promoter [PMID:8657130]"
/experiment="EXISTENCE:mutant phenotype:GO:0004722 protein
serine/threonine phosphatase activity [PMID:22235117]"
/experiment="EXISTENCE:mutant phenotype:GO:0006368
transcription elongation by RNA polymerase II
[PMID:17101794]"
/experiment="EXISTENCE:mutant phenotype:GO:0006369
termination of RNA polymerase II transcription
[PMID:22235117|PMID:12660165]"
/experiment="EXISTENCE:mutant phenotype:GO:0008420 RNA
polymerase II CTD heptapeptide repeat phosphatase activity
[PMID:15125841|PMID:22235117]"
/experiment="EXISTENCE:mutant phenotype:GO:0009302
sno(s)RNA transcription [PMID:12660165]"
/experiment="EXISTENCE:mutant phenotype:GO:0030846
termination of RNA polymerase II transcription,
poly(A)-coupled [PMID:12819204|PMID:12944462]"
/experiment="EXISTENCE:mutant phenotype:GO:0030847
termination of RNA polymerase II transcription,
exosome-dependent [PMID:12819204|PMID:12944462]"
/experiment="EXISTENCE:mutant phenotype:GO:0031124 mRNA
3'-end processing [PMID:12704082]"
/experiment="EXISTENCE:mutant phenotype:GO:0031564
transcription antitermination [PMID:12453421]"
/experiment="EXISTENCE:physical interaction:GO:0005634
nucleus [PMID:11046131]"
/experiment="EXISTENCE:physical interaction:GO:0005847
mRNA cleavage and polyadenylation specificity factor
complex [PMID:12453421]"
/experiment="EXISTENCE:physical interaction:GO:0006367
transcription initiation at RNA polymerase II promoter
[PMID:11046131]"
/note="Phosphatase and transcription/RNA-processing
factor; associates with TFIIB and cleavage/polyadenylation
factor Pta1p; exhibits phosphatase activity on serine-5
and serine-7 of the RNA polymerase II C-terminal domain;
affects start site selection and transcriptional read
through in vivo"
/codon_start=1
/product="RNA polymerase II subunit A C-terminal domain
phosphatase"
/protein_id="NP_014177.1"
/db_xref="GeneID:855499"
/db_xref="SGD:S000005166"
/translation="MPSHRNSNLKFCTVCASNNNRSMESHKVLQEAGYNVSSYGTGSA
VRLPGLSIDKPNVYSFGTPYNDIYNDLLSQSADRYKSNGLLQMLDRNRRLKKAPEKWQ
ESTKVFDFVFTCEERCFDAVCEDLMNRGGKLNKIVHVINVDIKDDDENAKIGSKAILE
LADMLNDKIEQCEKDDIPFEDCIMDILTEWQSSHSQLPSLYAPSYY"
gene complement(230105..230672)
/gene="SNR19"
/locus_tag="YNCN0005C"
/db_xref="GeneID:9164965"
ncRNA complement(230105..230672)
/ncRNA_class="snRNA"
/gene="SNR19"
/locus_tag="YNCN0005C"
/product="SNR19"
/experiment="EXISTENCE:direct assay:GO:0000243 commitment
complex [PMID:2529976]"
/experiment="EXISTENCE:direct assay:GO:0000395 mRNA
5'-splice site recognition [PMID:18084028]"
/experiment="EXISTENCE:direct assay:GO:0000398 mRNA
splicing, via spliceosome [PMID:2440584]"
/experiment="EXISTENCE:direct assay:GO:0005685 U1 snRNP
[PMID:2440584]"
/experiment="EXISTENCE:direct assay:GO:0030627 pre-mRNA
5'-splice site binding [PMID:18084028|PMID:12214237]"
/experiment="EXISTENCE:mutant phenotype:GO:0030627
pre-mRNA 5'-splice site binding
[PMID:15989965|PMID:3056718]"
/note="U1 spliceosomal RNA (U1 snRNA); snRNA U1 homolog"
/transcript_id="NR_132250.1"
/db_xref="GeneID:9164965"
/db_xref="SGD:S000007295"
gene complement(<231068..>233695)
/gene="POP1"
/locus_tag="YNL221C"
/db_xref="GeneID:855500"
mRNA complement(<231068..>233695)
/gene="POP1"
/locus_tag="YNL221C"
/product="ribonuclease P/MRP protein subunit POP1"
/transcript_id="NM_001183059.1"
/db_xref="GeneID:855500"
CDS complement(231068..233695)
/gene="POP1"
/locus_tag="YNL221C"
/EC_number="3.1.26.5"
/experiment="EXISTENCE:direct assay:GO:0000172
ribonuclease MRP complex [PMID:9620854]"
/experiment="EXISTENCE:direct assay:GO:0000294
nuclear-transcribed mRNA catabolic process, RNase
MRP-dependent [PMID:14729943]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:26135751|PMID:23700311]"
/experiment="EXISTENCE:direct assay:GO:0005655 nucleolar
ribonuclease P complex [PMID:9620854]"
/experiment="EXISTENCE:direct assay:GO:0005697 telomerase
holoenzyme complex [PMID:27156450]"
/experiment="EXISTENCE:direct assay:GO:0034965 intronic
box C/D snoRNA processing [PMID:18713869]"
/experiment="EXISTENCE:mutant phenotype:GO:0000171
ribonuclease MRP activity [PMID:16618965]"
/experiment="EXISTENCE:mutant phenotype:GO:0004526
ribonuclease P activity [PMID:16618965]"
/experiment="EXISTENCE:mutant phenotype:GO:0006364 rRNA
processing [PMID:7926742]"
/experiment="EXISTENCE:mutant phenotype:GO:0008033 tRNA
processing [PMID:7926742]"
/experiment="EXISTENCE:physical interaction:GO:0000172
ribonuclease MRP complex [PMID:17881380]"
/note="Subunit of RNase MRP, nuclear RNase P and
telomerase complexes; RNase MRP cleaves pre-rRNA, nuclear
RNase P cleaves tRNA precursors to generate mature 5' ends
and facilitates turnover of nuclear RNAs, while telomerase
replenishes telomeric DNA; binds to the RPR1 RNA subunit
in RNase P"
/codon_start=1
/product="ribonuclease P/MRP protein subunit POP1"
/protein_id="NP_014178.1"
/db_xref="GeneID:855500"
/db_xref="SGD:S000005165"
/translation="MSGSLSRGNGGKKVLNKNQLLKRNRIRNARSIRAEAVAASSTKT
GTPSDLSESGSKLNVDQFISSRQFEVKQLQLAMHNSKAASSTRIFQALPRKLRRRTAS
HNVRRIPKRMRNRALREMRKSDQQDVLKGSSASSRKAHGLNAKQLYKARMSIKLLRLA
SKSTSMKLSMPPEVTSSNCHVRQKIKTLKRMIKESSTANPNIKLLNNRMGSYDCTGVN
ELAPIPKGRVKYTKRQKHFAWLPTHIWNAKRSHMMKRWGYQMVWAPTQKCFKLTHRLG
GDTCSSDGALCMDSSYIGTIIVKDKSNDSEGDFLKSIIGKLTAERANLRKYREGQVLF
QGLIYSFNEENGEDSTKPLGPCDVFWVQKDTAIIRLHPSIYTQVFNILLQHKEKLTVQ
DCRYSLASVTLKGAKALESLASCLRSTEYSKSFEQFKMVSMITDHNALPQRCTFAFEA
IDPRHLAAPKKLNDSQRKTVNSDDILSLHENYPQDEINAVFNELCDPESRTQSYNNQN
TLKEISARRYKLLTATPNSINKTTVPFKESDDPSIPLVIIRRLKTRDWIVVLPWFWLL
PLWHLLNRIPRMYHIGLRQFQQIQYENKQLYFPDDYPFTQLGYIENSFYKKEASKTKW
DRKPMGKRINFEKIKDIHNTKLPAYSGEIGDFFSSDWRFLQILRNGIDYLQRNDKTLE
LMDSKKTGQFNAQGVRDINCVNDVLEFCKDYEAKTKAMSLSIEENIPVALCKNRKCQF
RTPDSISVNSSSFSLTFFPRCIIAVSCTLLERGHPKDNARIYQVPEKDLEHWLQLAKG
VYRPNGRKDHDLKIPLPEVHDLIGFITSGTYHLNCGNGMGIGFIDHHAAIRQPTRYVL
IRNVGTNTYRLGEWSKISV"
gene <234413..>235714
/gene="ADE12"
/locus_tag="YNL220W"
/gene_synonym="BRA9"
/db_xref="GeneID:855501"
mRNA <234413..>235714
/gene="ADE12"
/locus_tag="YNL220W"
/gene_synonym="BRA9"
/product="adenylosuccinate synthase"
/transcript_id="NM_001183058.1"
/db_xref="GeneID:855501"
CDS 234413..235714
/gene="ADE12"
/locus_tag="YNL220W"
/gene_synonym="BRA9"
/EC_number="6.3.4.4"
/experiment="EXISTENCE:direct assay:GO:0003688 DNA
replication origin binding [PMID:8706758]"
/experiment="EXISTENCE:direct assay:GO:0004019
adenylosuccinate synthase activity
[PMID:8706758|PMID:10417315]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0004019
adenylosuccinate synthase activity [PMID:5807803]"
/experiment="EXISTENCE:mutant phenotype:GO:0006164 purine
nucleotide biosynthetic process [PMID:5807803]"
/note="Adenylosuccinate synthase; catalyzes the first step
in synthesis of adenosine monophosphate from inosine
5'monophosphate during purine nucleotide biosynthesis;
exhibits binding to single-stranded autonomously
replicating (ARS) core sequence"
/codon_start=1
/product="adenylosuccinate synthase"
/protein_id="NP_014179.1"
/db_xref="GeneID:855501"
/db_xref="SGD:S000005164"
/translation="MVNVVLGSQWGDEGKGKLVDLLVGKYDIVARCAGGNNAGHTIVV
DGVKYDFHMLPSGLVNPNCQNLLGNGVVIHVPSFFKELETLEAKGLKNARSRLFVSSR
AHLVFDFHQVTDKLRELELSGRSKDGKNIGTTGKGIGPTYSTKASRSGLRVHHLVNDQ
PGAWEEFVARYKRLLETRRQRYGDFEYDFEAKLAEYKKLREQLKPFVVDSVVFMHNAI
EAKKKILVEGANALMLDIDFGTYPYVTSSNTGIGGVLTGLGIPPRTIDEIYGVVKAYT
TRVGEGPFPTEQLNENGEKLQTIGAEFGVTTGRKRRCGWLDLVVLKYSTLINGYTSLN
ITKLDVLDTFKEIPVGISYSIQGKKLDLFPEDLNILGKVEVEYKVLPGWDQDITKITK
YEDLPENAKKYLKYIEDFVGVPVEWVGTGPARESMLHKEIK"
gene complement(<235996..>237663)
/gene="ALG9"
/locus_tag="YNL219C"
/db_xref="GeneID:855502"
mRNA complement(<235996..>237663)
/gene="ALG9"
/locus_tag="YNL219C"
/product="dolichyl-P-Man:Man(6)GlcNAc(2)-PP-dolichol
alpha-1,2-mannosyltransferase"
/transcript_id="NM_001183057.1"
/db_xref="GeneID:855502"
CDS complement(235996..237663)
/gene="ALG9"
/locus_tag="YNL219C"
/EC_number="2.4.1.259"
/EC_number="2.4.1.261"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0006487 protein
N-linked glycosylation [PMID:15987956]"
/experiment="EXISTENCE:direct assay:GO:0052918
dol-P-Man:Man(8)GlcNAc(2)-PP-Dol
alpha-1,2-mannosyltransferase activity [PMID:15987956]"
/experiment="EXISTENCE:direct assay:GO:0052926
dol-P-Man:Man(6)GlcNAc(2)-PP-Dol
alpha-1,2-mannosyltransferase activity [PMID:15987956]"
/experiment="EXISTENCE:mutant phenotype:GO:0000030
mannosyltransferase activity [PMID:8692962]"
/experiment="EXISTENCE:mutant phenotype:GO:0005783
endoplasmic reticulum [PMID:8692962]"
/experiment="EXISTENCE:mutant phenotype:GO:0006488
dolichol-linked oligosaccharide biosynthetic process
[PMID:15987956]"
/experiment="EXISTENCE:mutant phenotype:GO:0009101
glycoprotein biosynthetic process [PMID:8692962]"
/note="Mannosyltransferase, involved in N-linked
glycosylation; catalyzes the transfer of both the seventh
mannose residue on B-arm and ninth mannose residue on the
C-arm from Dol-P-Man to lipid-linked oligosaccharides;
human homolog ALG9 can complement yeast null mutant;
mutation of human homolog causes type 1 congenital
disorders of glycosylation"
/codon_start=1
/product="dolichyl-P-Man:Man(6)GlcNAc(2)-PP-dolichol
alpha-1,2-mannosyltransferase"
/protein_id="NP_014180.1"
/db_xref="GeneID:855502"
/db_xref="SGD:S000005163"
/translation="MNCKAVTISLLLLLFLTRVYIQPTFSLISDCDETFNYWEPLNLL
VRGFGKQTWEYSPEYSIRSWAFLLPFYCILYPVNKFTDLESHWNFFITRACLGFFSFI
MEFKLHREIAGSLALQIANIWIIFQLFNPGWFHASVELLPSAVAMLLYVGATRHSLRY
LSTGSTSNFTKSLAYNFLASILGWPFVLILSLPLCLHYLFNHRIISTIRTAFDCCLIF
SLTAFAVIVTDSIFYGKLAPVSWNILFYNVINASEESGPNIFGVEPWYYYPLNLLLNF
PLPVLVLAILGIFHLRLWPLWASLFTWIAVFTQQPHKEERFLYPIYGLITLSASIAFY
KVLNLFNRKPILKKGIKLSVLLIVAGQAMSRIVALVNNYTAPIAVYEQFSSLNQGGVK
APVVNVCTGREWYHFPSSFLLPDNHRLKFVKSGFDGLLPGDFPESGSIFKKIRTLPKG
MNNKNIYDTGKEWPITRCDYFIDIVAPINLTKDVFNPLHLMDNWNKLACAAFIDGENS
KILGRAFYVPEPINRIMQIVLPKQWNQVYGVRYIDYCLFEKPTETTN"
gene <238238..>240001
/gene="MGS1"
/locus_tag="YNL218W"
/db_xref="GeneID:855503"
mRNA <238238..>240001
/gene="MGS1"
/locus_tag="YNL218W"
/product="ssDNA-dependent ATPase MGS1"
/transcript_id="NM_001183056.1"
/db_xref="GeneID:855503"
CDS 238238..240001
/gene="MGS1"
/locus_tag="YNL218W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008047 enzyme
activator activity [PMID:16251400]"
/experiment="EXISTENCE:direct assay:GO:0017116
single-stranded DNA helicase activity
[PMID:11459965|PMID:16251400]"
/experiment="EXISTENCE:direct assay:GO:0033567 DNA
replication, Okazaki fragment processing [PMID:16251400]"
/experiment="EXISTENCE:direct assay:GO:0051880
G-quadruplex DNA binding [PMID:32748509]"
/experiment="EXISTENCE:genetic interaction:GO:0006282
regulation of DNA repair [PMID:16809783]"
/experiment="EXISTENCE:mutant phenotype:GO:0051276
chromosome organization [PMID:32748509]"
/note="Protein with DNA-dependent ATPase and ssDNA
annealing activities; involved in maintenance of genome;
interacts functionally with DNA polymerase delta; homolog
of human Werner helicase interacting protein (WHIP); forms
nuclear foci upon DNA replication stress"
/codon_start=1
/product="ssDNA-dependent ATPase MGS1"
/protein_id="NP_014181.1"
/db_xref="GeneID:855503"
/db_xref="SGD:S000005162"
/translation="MSNKRTSVEQLISCPICSRKVFFSLINSHLDICGKEKSKPSSRP
QTVSSLLAGPKKRKQANSEKFIDLENKDHEIKPGLKSESDDIEIVENESKRFKAAPST
DFAKSIVEPASSRDQLHNDYESRWLQKISHLPLSEKLRPKELRDYVGQQHILSQDNGT
LFKYIKQGTIPSMILWGPPGVGKTSLARLLTKTATTSSNESNVGSRYFMIETSATKAN
TQELRGIFEKSKKEYQLTKRRTVLFIDEIHRFNKVQQDLLLPHVENGDIILIGATTEN
PSFQLNNALISRCLIFVLEKLNVNELCIVLSRGIALLNKCRKQVWNIENPLKLSRSIL
EYVVDLSVGDTRRALNMLEMIEVSTRERKADEEELSIDDVRDIIKNNSSNGLNTYYDP
KGDNHYDTISAFHKSIRGGDENASLYYLARMLQGGEDPLYVARRMIRIASEDIGLRDS
SLLPLAVAAHDAVMKVGLPEADLALAQCCVALARAPKSVELYRAWKKLRAMMSENMYS
LASSEIPMHIRNAPTKLMEELGYHKGYKYNPDYIEGKVQQDYFPKEVLEKCPNKTDLK
FLDGKHLGDKEDPDLRQSYQG"
gene <240331..>241311
/gene="PPN2"
/locus_tag="YNL217W"
/db_xref="GeneID:855504"
mRNA <240331..>241311
/gene="PPN2"
/locus_tag="YNL217W"
/product="putative serine/threonine-protein phosphatase"
/transcript_id="NM_001183055.1"
/db_xref="GeneID:855504"
CDS 240331..241311
/gene="PPN2"
/locus_tag="YNL217W"
/EC_number="3.6.1.10"
/experiment="EXISTENCE:direct assay:GO:0000298
endopolyphosphatase activity
[PMID:28302909|PMID:31175919]"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:14562095|PMID:23708375]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005775 vacuolar
lumen [PMID:28302909]"
/experiment="EXISTENCE:direct assay:GO:0006798
polyphosphate catabolic process [PMID:28302909]"
/experiment="EXISTENCE:genetic interaction:GO:0000298
endopolyphosphatase activity [PMID:28302909]"
/experiment="EXISTENCE:genetic interaction:GO:0006798
polyphosphate catabolic process [PMID:28302909]"
/experiment="EXISTENCE:genetic interaction:GO:0016036
cellular response to phosphate starvation [PMID:28302909]"
/experiment="EXISTENCE:mutant phenotype:GO:0000298
endopolyphosphatase activity [PMID:28302909]"
/experiment="EXISTENCE:mutant phenotype:GO:0006798
polyphosphate catabolic process
[PMID:28302909|PMID:39590716]"
/note="Zn2+-dependent endopolyphosphatase; required with
PPN1 to mobilize polyphosphate stores in response to
phosphate starvation; member of the PPP-superfamily of
metalloproteases; directly involved in adaptation to mild
alkaline conditions through polyphosphate hydrolysis;
localizes to the vacuolar lumen via the MVB pathway; null
mutant is highly sensitive to azaserine and resistant to
sodium-O-vandate"
/codon_start=1
/product="putative serine/threonine-protein phosphatase"
/protein_id="NP_014182.1"
/db_xref="GeneID:855504"
/db_xref="SGD:S000005161"
/translation="MEDKRKRRAATLSTALILFVACCVYTLYIFKFDNPRLSPPVSLL
PTISTLKKIEHVTDLNKEYVFVGDVHGNYDEFIELIDDKIGGLGENITMILLGDFIHK
GPDSDKVVSYILNHKDQVKCVLGNHEILVMMAYLNPDFSKWVRRPKLMTPLTFSTETN
FIPQDISKISNAHGRLARELGFSKLSQLAEHCSMAIELDLDITGDILFGAHAGMVPGD
FMKPNQIPGVSSLSNMKYVDKKNWSKTSREKENKNYVRWYTLWDKYGDHFSNAKVFYG
HDASMGLNLRRQTKGLDTACIKNNLLSSMKVKYDIKKGQYDYELIQVQCS"
gene <241689..>244172
/gene="RAP1"
/locus_tag="YNL216W"
/gene_synonym="GRC4; GRF1; TBA1; TUF1"
/db_xref="GeneID:855505"
mRNA <241689..>244172
/gene="RAP1"
/locus_tag="YNL216W"
/gene_synonym="GRC4; GRF1; TBA1; TUF1"
/product="DNA-binding transcription factor RAP1"
/transcript_id="NM_001183054.1"
/db_xref="GeneID:855505"
CDS 241689..244172
/gene="RAP1"
/locus_tag="YNL216W"
/gene_synonym="GRC4; GRF1; TBA1; TUF1"
/experiment="EXISTENCE:direct assay:GO:0000228 nuclear
chromosome [PMID:11455386]"
/experiment="EXISTENCE:direct assay:GO:0000781 chromosome,
telomeric region
[PMID:16956377|PMID:26399229|PMID:9710643]"
/experiment="EXISTENCE:direct assay:GO:0000981 DNA-binding
transcription factor activity, RNA polymerase II-specific
[PMID:1904543]"
/experiment="EXISTENCE:direct assay:GO:0000987
cis-regulatory region sequence-specific DNA binding
[PMID:12051841|PMID:10487921]"
/experiment="EXISTENCE:direct assay:GO:0001094 TFIID-class
transcription factor complex binding [PMID:17074814]"
/experiment="EXISTENCE:direct assay:GO:0003700 DNA-binding
transcription factor activity
[PMID:20559389|PMID:18195009]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0008301 DNA
binding, bending [PMID:2656680]"
/experiment="EXISTENCE:direct assay:GO:0010833 telomere
maintenance via telomere lengthening [PMID:9020083]"
/experiment="EXISTENCE:direct assay:GO:0017025 TBP-class
protein binding [PMID:18195009]"
/experiment="EXISTENCE:direct assay:GO:0031492 nucleosomal
DNA binding [PMID:19782036]"
/experiment="EXISTENCE:direct assay:GO:0042162 telomeric
DNA binding [PMID:17656141|PMID:8510148|PMID:2225074]"
/experiment="EXISTENCE:direct assay:GO:0042393 histone
binding [PMID:31742863]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:19158363]"
/experiment="EXISTENCE:direct assay:GO:0051880
G-quadruplex DNA binding [PMID:8194531]"
/experiment="EXISTENCE:direct assay:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:20559389]"
/experiment="EXISTENCE:direct assay:GO:0061849 telomeric
G-quadruplex DNA binding [PMID:8052638]"
/experiment="EXISTENCE:direct assay:GO:0070187 shelterin
complex [PMID:23746845]"
/experiment="EXISTENCE:direct assay:GO:0071919
G-quadruplex DNA formation [PMID:8052638]"
/experiment="EXISTENCE:genetic interaction:GO:0006110
regulation of glycolytic process [PMID:15300680]"
/experiment="EXISTENCE:genetic interaction:GO:0030466
silent mating-type cassette heterochromatin formation
[PMID:7958893]"
/experiment="EXISTENCE:genetic interaction:GO:0031509
subtelomeric heterochromatin formation [PMID:7958893]"
/experiment="EXISTENCE:genetic interaction:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:15300680]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:2010087]"
/experiment="EXISTENCE:mutant phenotype:GO:0000723
telomere maintenance [PMID:2237406]"
/experiment="EXISTENCE:mutant phenotype:GO:0000781
chromosome, telomeric region [PMID:27122604]"
/experiment="EXISTENCE:mutant phenotype:GO:0010833
telomere maintenance via telomere lengthening
[PMID:9020083]"
/experiment="EXISTENCE:mutant phenotype:GO:0031848
protection from non-homologous end joining at telomere
[PMID:16096640]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:2010087]"
/experiment="EXISTENCE:physical interaction:GO:0031509
subtelomeric heterochromatin formation [PMID:12080091]"
/experiment="EXISTENCE:physical interaction:GO:0070200
establishment of protein localization to telomere
[PMID:11689698]"
/experiment="EXISTENCE:physical interaction:GO:0071169
establishment of protein localization to chromatin
[PMID:11689698]"
/note="DNA-binding transcription regulator; involved in
transcription activation, repression, chromatin silencing,
telomere length maintenance; represses divergent noncoding
transcription at highly expressed genes, prevents
initiation at cryptic promoters; recruits Sir complex to
telomeric DNA; forms telomere hyperclusters in quiescent
cells; arrays of telomere-bound Rap1p stall
condensin-induced DNA loop extrusion causing local
chromatin decompaction in anaphase; relocalizes to cytosol
under hypoxia"
/codon_start=1
/product="DNA-binding transcription factor RAP1"
/protein_id="NP_014183.1"
/db_xref="GeneID:855505"
/db_xref="SGD:S000005160"
/translation="MSSPDDFETAPAEYVDALDPSMVVVDSGSAAVTAPSDSAAEVKA
NQNEENTGATAAETSEKVDQTEVEKKDDDDTTEVGVTTTTPSIADTAATANIASTSGA
SVTEPTTDDTAADEKKEQVSGPPLSNMKFYLNRDADAHDSLNDIDQLARLIRANGGEV
LDSKPRESKENVFIVSPYNHTNLPTVTPTYIKACCQSNSLLNMENYLVPYDNFREVVD
SRLQEESHSNGVDNSNSNSDNKDSIRPKTEIISTNTNGATEDSTSEKVMVDAEQQARL
QEQAQLLRQHVSSTASITSGGHNDLVQIEQPQKDTSNNNNSNVNDEDNDLLTQDNNPQ
TADEGNASFQAQRSMISRGALPSHNKASFTDEEDEFILDVVRKNPTRRTTHTLYDEIS
HYVPNHTGNSIRHRFRVYLSKRLEYVYEVDKFGKLVRDDDGNLIKTKVLPPSIKRKFS
ADEDYTLAIAVKKQFYRDLFQIDPDTGRSLITDEDTPTAIARRNMTMDPNHVPGSEPN
FAAYRTQSRRGPIAREFFKHFAEEHAAHTENAWRDRFRKFLLAYGIDDYISYYEAEKA
QNREPEPMKNLTNRPKRPGVPTPGNYNSAAKRARNYSSQRNVQPTANAASANAAAAAA
AAASNSYAIPENELLDEDTMNFISSLKNDLSNISNSLPFEYPHEIAEAIRSDFSNEDI
YDNIDPDTISFPPKIATTDLFLPLFFHFGSTRQFMDKLHEVISGDYEPSQAEKLVQDL
CDETGIRKNFSTSILTCLSGDLMVFPRYFLNMFKDNVNPPPNVPGIWTHDDDESLKSN
DQEQIRKLVKKHGTGRMEMRKRFFEKDLL"
gene <244468..>245430
/gene="IES2"
/locus_tag="YNL215W"
/db_xref="GeneID:855506"
mRNA <244468..>245430
/gene="IES2"
/locus_tag="YNL215W"
/product="Ies2p"
/transcript_id="NM_001183053.1"
/db_xref="GeneID:855506"
CDS 244468..245430
/gene="IES2"
/locus_tag="YNL215W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:physical interaction:GO:0031011
Ino80 complex [PMID:24034245]"
/note="Protein that associates with the INO80 chromatin
remodeling complex; associates with the INO80 complex
under low-salt conditions; essential for growth under
anaerobic conditions; protein abundance increases in
response to DNA replication stress"
/codon_start=1
/product="Ies2p"
/protein_id="NP_014184.1"
/db_xref="GeneID:855506"
/db_xref="SGD:S000005159"
/translation="MDSEASDIEAELSDSVSAGGEEYIDDDDYTEDIDDQIVTAKSSR
RTARRSVPKGVRTSKRIRDKELSVEVDEDYDEEEDVLSPSKKRHLHTRSMDKRQVAAT
ASEKSDIGDSKGNDGEIEDGILEEEESLEKELNRGGGKEVEKSEESYYAQNDVGQKGE
EEQDGESGGYEDNEPSISKESDELVSVVNGNGNEEDDEVEATKENTTDSTRSTTTRSK
MLLDLLEDGGSKKKLTDEEIQLRRAENARKRKNLSEKRLEEEKQDTINKLLKKRAGKS
RSHLPNDDEKNDGSSSFVKPRRPYNSEGMTRILRRYEEDLFCTF"
gene <245617..>246216
/gene="PEX17"
/locus_tag="YNL214W"
/gene_synonym="PAS9"
/db_xref="GeneID:855507"
mRNA <245617..>246216
/gene="PEX17"
/locus_tag="YNL214W"
/gene_synonym="PAS9"
/product="Pex17p"
/transcript_id="NM_001183052.1"
/db_xref="GeneID:855507"
CDS 245617..246216
/gene="PEX17"
/locus_tag="YNL214W"
/gene_synonym="PAS9"
/experiment="EXISTENCE:direct assay:GO:0005778 peroxisomal
membrane [PMID:9094717|PMID:9425153]"
/experiment="EXISTENCE:direct assay:GO:0008320 protein
transmembrane transporter activity [PMID:20154681]"
/experiment="EXISTENCE:direct assay:GO:0016561 protein
import into peroxisome matrix, translocation
[PMID:20154681]"
/experiment="EXISTENCE:direct assay:GO:1990429 peroxisomal
importomer complex
[PMID:20154681|PMID:12667447|PMID:22375831]"
/experiment="EXISTENCE:mutant phenotype:GO:0005778
peroxisomal membrane [PMID:9425153]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding
[PMID:12667447|PMID:9425153|PMID:16679311]"
/experiment="EXISTENCE:physical interaction:GO:0016560
protein import into peroxisome matrix, docking
[PMID:16679311]"
/note="Membrane peroxin of the peroxisomal importomer
complex; complex facilitates the import of peroxisomal
matrix proteins; required for peroxisome biogenesis"
/codon_start=1
/product="Pex17p"
/protein_id="NP_014185.1"
/db_xref="GeneID:855507"
/db_xref="SGD:S000005158"
/translation="MTSINSFPRNIDWPSNIGIKKIEGTNPTVNAIKGLLYNGGSIYA
FLYFVIAMFVEPTLQKQYQQRNDFSLFVLLRLRRIIAQLQKRLVMTPVSSLGFNEQNN
FVERSTQTSDDNIIREDNSHWAEMIYQLQNMKQELQYFNRSSGQPSESIDDFVFQIKM
VTDQVELTDRSRAFSNKSRNIIQGIREIKGWFVNGQVPR"
gene complement(<246460..>247104)
/gene="RRG9"
/locus_tag="YNL213C"
/db_xref="GeneID:855508"
mRNA complement(<246460..>247104)
/gene="RRG9"
/locus_tag="YNL213C"
/product="mitochondrial ribosome assembly protein RRG9"
/transcript_id="NM_001183051.1"
/db_xref="GeneID:855508"
CDS complement(246460..247104)
/gene="RRG9"
/locus_tag="YNL213C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:mutant phenotype:GO:0007005
mitochondrion organization [PMID:10029995]"
/note="hypothetical protein; null mutant lacks
mitochondrial DNA and cannot grow on glycerol; the
authentic, non-tagged protein is detected in highly
purified mitochondria in high-throughput studies"
/codon_start=1
/product="mitochondrial ribosome assembly protein RRG9"
/protein_id="NP_014186.1"
/db_xref="GeneID:855508"
/db_xref="SGD:S000005157"
/translation="MNILRIACRSFHCLRCGPLLNENRGWSSKKIIKLVNKSSLSNKE
FTEKVRDGTKDIPEWKKQKMAVRKKLQGQRWNPPKKISQEQMEALRLLKFNFPELTAS
DLADRFKISPEAVRRILKSNWKRTDEENNNTYERWKRRGERIKEMYQRKEDADFVSNQ
IVTSRKIILGSNSNSPELIARNVRTFKPFKPNNSTPEKKNTNKLYILKHLGSKQ"
gene <247461..>249809
/gene="VID27"
/locus_tag="YNL212W"
/db_xref="GeneID:855509"
mRNA <247461..>249809
/gene="VID27"
/locus_tag="YNL212W"
/product="Vid27p"
/transcript_id="NM_001183050.1"
/db_xref="GeneID:855509"
CDS 247461..249809
/gene="VID27"
/locus_tag="YNL212W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="Cytoplasmic hypothetical protein; possibly involved
in vacuolar protein degradation; not essential for
proteasome-dependent degradation of
fructose-1,6-bisphosphatase (FBPase); null mutants exhibit
normal growth; contains two PH-like domains"
/codon_start=1
/product="Vid27p"
/protein_id="NP_014187.1"
/db_xref="GeneID:855509"
/db_xref="SGD:S000005156"
/translation="MNILKKFMESGNKPELITIPSGQFNLLRSKNSPKAALECIYNNA
TLSVRKIGKFDYELAVYRVEDDSEGGTGDEAENFEDDTISVLSTQSKKKEEEWSVEIS
DKIMFHKTWDKQGNVALVWENLRGDEQDEKVQFVVAADVSFSDVEQFIQTVYRCQFEV
RNKKSSLTASADDLKEIEHRSTRLFVQDDDDELDSSSDDFQDAKDTSFEHEKESEILE
RTPSPLKKVPEGEYCCLVMSSLYMYDPIQEKFILQEPVVKVAIIDTGKYEFWLAIEGK
DNRLGTQVAPNINPTFELATDAFLFNYTLQNITLSYMLKFKDLDKCIQFRFAWVKCLW
MTLNKETWTDVPEKEKDYILDSSSVPLEKQFDDILHIDDRSNEERDKESSESENDSED
EDDENDHSKRIISSEAFEEPRRATSKGNSSLTVAFRNNRSYVTRDNRIGVFKTDDEDD
SLEFVAAIKNISNLGGKSIDPHKPMLYMEDRNLILTDGENENKLYKMDIERGKVIEEW
STGDKNVVQYGPTKKFDQMTPEQTIVGVSQKGVFKIDPRINGKNKIAVDESKDYVGKY
NFSSIGTTESGYIAIGSEKGDIKLYDRLGIRAKTAIPSLGQAIKFITTSADGKWLLAT
CESTLLLMDLKIKDGKNAGNIGFLKSFPASENVKTYVLKIRPEHSASILTYTKKPIRF
TKAYFNTGIGQQEQTIVTSTGPYAISWSLKGILNQDGSNNYPYRIRRYNADVVADNFE
FGSDKKVIVALKDDVSLSKVKSFKQPSKGVLMPSASLQDFYG"
gene complement(<250055..>250315)
/gene="MRX7"
/locus_tag="YNL211C"
/db_xref="GeneID:855510"
mRNA complement(<250055..>250315)
/gene="MRX7"
/locus_tag="YNL211C"
/product="Mrx7p"
/transcript_id="NM_001183049.1"
/db_xref="GeneID:855510"
CDS complement(250055..250315)
/gene="MRX7"
/locus_tag="YNL211C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095]"
/note="Protein that associates with mitochondrial
ribosome; green fluorescent protein (GFP)-fusion protein
localizes to mitochondria; YNL211C is not an essential
gene"
/codon_start=1
/product="Mrx7p"
/protein_id="NP_014188.1"
/db_xref="GeneID:855510"
/db_xref="SGD:S000005155"
/translation="MPPRSIEEWFYYKLLSSPGFHRFVRKVYRKVNGIKEDPFTDQST
AFQYLYKPTPRQKFKALRLLFWDEMRSTFGFRRRLGDRFKKD"
rep_origin 250315..250931
/note="ARS1413; Strong origin of replication activated in
late S phase"
/db_xref="SGD:S000077375"
gene <250931..>251743
/gene="MER1"
/locus_tag="YNL210W"
/db_xref="GeneID:855511"
mRNA <250931..>251743
/gene="MER1"
/locus_tag="YNL210W"
/product="Mer1p"
/transcript_id="NM_001183048.1"
/db_xref="GeneID:855511"
CDS 250931..251743
/gene="MER1"
/locus_tag="YNL210W"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:10983980]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:7891689]"
/experiment="EXISTENCE:direct assay:GO:1990446 U1 snRNP
binding [PMID:10983980]"
/experiment="EXISTENCE:genetic interaction:GO:0007131
reciprocal meiotic recombination [PMID:2659434]"
/experiment="EXISTENCE:mutant phenotype:GO:0007131
reciprocal meiotic recombination [PMID:2659434]"
/experiment="EXISTENCE:mutant phenotype:GO:0048026
positive regulation of mRNA splicing, via spliceosome
[PMID:10983980]"
/note="mRNA-binding protein required for meiosis-specific
mRNA splicing; required for chromosome pairing and meiotic
recombination; Mer1p regulon embraces four essential
meiotic pre-mRNAs: REC107, HFM1, AMA1 and SPO22"
/codon_start=1
/product="Mer1p"
/protein_id="NP_014189.1"
/db_xref="GeneID:855511"
/db_xref="SGD:S000005154"
/translation="MSNQHSPQPFCLDTKLVKLLEELQEGKQFNNKNIFPEKALYLKL
ALDYSFFRKNLLEFCVHLDKIKGVIRPNYDTIYILCLLEVDLLNLVFTDNILEICLPR
FVSREDLRVFNNTFYTYHDNRLRILQEDFSQLFKKIKTKASVLCFTVEEIFLTNQEIL
PQNSTVAELQKSTNKVQTNGPQRHDFIVTLEIKLNKTQITFLIGAKGTRIESLREKSG
ASIKIIPISDKMTAHERNHPESVQQTILISGDLYSIALAVTSIESALITLDL"
gene <252059..>253900
/gene="SSB2"
/locus_tag="YNL209W"
/gene_synonym="YG103"
/db_xref="GeneID:855512"
mRNA <252059..>253900
/gene="SSB2"
/locus_tag="YNL209W"
/gene_synonym="YG103"
/product="Hsp70 family ATPase SSB2"
/transcript_id="NM_001183047.1"
/db_xref="GeneID:855512"
CDS 252059..253900
/gene="SSB2"
/locus_tag="YNL209W"
/gene_synonym="YG103"
/EC_number="3.6.4.10"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:9860955]"
/experiment="EXISTENCE:direct assay:GO:0051082 unfolded
protein binding [PMID:9670014]"
/experiment="EXISTENCE:direct assay:GO:0051083 'de novo'
cotranslational protein folding [PMID:9670014]"
/experiment="EXISTENCE:genetic interaction:GO:0000054
ribosomal subunit export from nucleus [PMID:20368619]"
/experiment="EXISTENCE:genetic interaction:GO:0006364 rRNA
processing [PMID:20368619]"
/experiment="EXISTENCE:genetic interaction:GO:0042149
cellular response to glucose starvation [PMID:19723765]"
/experiment="EXISTENCE:mutant phenotype:GO:0002181
cytoplasmic translation [PMID:1394434]"
/experiment="EXISTENCE:mutant phenotype:GO:0006450
regulation of translational fidelity [PMID:15456889]"
/experiment="EXISTENCE:mutant phenotype:GO:0006452
translational frameshifting [PMID:16607023]"
/experiment="EXISTENCE:physical interaction:GO:0002181
cytoplasmic translation [PMID:1394434]"
/note="Cytoplasmic ATPase that is a ribosome-associated
molecular chaperone; functions with J-protein partner
Zuo1p; may be involved in the folding of newly-synthesized
polypeptide chains; member of the HSP70 family; SSB2 has a
paralog, SSB1, that arose from the whole genome
duplication"
/codon_start=1
/product="Hsp70 family ATPase SSB2"
/protein_id="NP_014190.1"
/db_xref="GeneID:855512"
/db_xref="SGD:S000005153"
/translation="MAEGVFQGAIGIDLGTTYSCVATYESSVEIIANEQGNRVTPSFV
AFTPQERLIGDAAKNQAALNPRNTVFDAKRLIGRRFDDESVQKDMKTWPFKVIDVDGN
PVIEVQYLEETKTFSPQEISAMVLTKMKEIAEAKIGKKVEKAVITVPAYFNDAQRQAT
KDAGAISGLNVLRIINEPTAAAIAYGLGAGKSEKERHVLIFDLGGGTFDVSLLHIAGG
VYTVKSTSGNTHLGGQDFDTNLLEHFKAEFKKKTGLDISDDARALRRLRTAAERAKRT
LSSVTQTTVEVDSLFDGEDFESSLTRARFEDLNAALFKSTLEPVEQVLKDAKISKSQI
DEVVLVGGSTRIPKVQKLLSDFFDGKQLEKSINPDEAVAYGAAVQGAILTGQSTSDET
KDLLLLDVAPLSLGVGMQGDIFGIVVPRNTTVPTIKRRTFTTVSDNQTTVQFPVYQGE
RVNCKENTLLGEFDLKNIPMMPAGEPVLEAIFEVDANGILKVTAVEKSTGKSSNITIS
NAVGRLSSEEIEKMVNQAEEFKAADEAFAKKHEARQRLESYVASIEQTVTDPVLSSKL
KRGSKSKIEAALSDALAALQIEDPSADELRKAEVGLKRVVTKAMSSR"
gene <254418..>255017
/locus_tag="YNL208W"
/db_xref="GeneID:855513"
mRNA <254418..>255017
/locus_tag="YNL208W"
/product="uncharacterized protein"
/transcript_id="NM_001183046.1"
/db_xref="GeneID:855513"
CDS 254418..255017
/locus_tag="YNL208W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/note="hypothetical protein; may interact with ribosomes,
based on co-purification experiments; authentic,
non-tagged protein is detected in purified mitochondria in
high-throughput studies; potential orthologs found in
other fungi"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014191.2"
/db_xref="GeneID:855513"
/db_xref="SGD:S000005152"
/translation="MSANEFYSSGQQGQYNQQNNQERTGAPNNGQYGADNGNPNGERG
LFSTIVGGSAGAYAGSKVSNNHSKLSGVLGAIGGAFLANKISDERKEHKQQEQYGNSN
FGGAPQGGHNNHHRQDNNNNNGGFGGPGGPGGQGFGRQGPQGFGGPGPQEFGGPGGQG
FGGPNPQEFGGPGGQGFGGPNPQEFGGQGRQGFNGGSRW"
gene <255353..>256630
/gene="RIO2"
/locus_tag="YNL207W"
/db_xref="GeneID:855514"
mRNA <255353..>256630
/gene="RIO2"
/locus_tag="YNL207W"
/product="protein kinase RIO2"
/transcript_id="NM_001183045.1"
/db_xref="GeneID:855514"
CDS 255353..256630
/gene="RIO2"
/locus_tag="YNL207W"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:12690111]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12690111|PMID:12612080]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:15167894]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:12612080]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405]"
/experiment="EXISTENCE:mutant phenotype:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:12612080|PMID:12690111]"
/experiment="EXISTENCE:mutant phenotype:GO:0046830
positive regulation of RNA import into nucleus
[PMID:12612080]"
/note="Essential serine kinase involved in the processing
of 20S pre-rRNA; involved in the processing of the 20S
pre-rRNA into mature 18S rRNA; has similarity to Rio1p"
/codon_start=1
/product="protein kinase RIO2"
/protein_id="NP_014192.1"
/db_xref="GeneID:855514"
/db_xref="SGD:S000005151"
/translation="MKLDTSHMRYLTTDDFRVLQAVEQGSRSHEVVPTPLIHQISGMR
SQSGTNRAISDLAKLSLISKMRNVKYDGYRLTYNGIDYLALKTMLNRDTVYSVGNTIG
VGKESDIYKVSDKNGNPRVMKIHRLGRTSFHSVRNNRDYLKKSNQGANWMHLSRLAAN
KEYQFMSMLYSKGFKVPEPFDNSRHIVVMELIEGYPMRRLRKHKNIPKLYSDLMCFIV
DLANSGLIHCDFNEFNIMIKDKLEDENDCGFVVIDFPQCISIQHQDADYYFQRDVDCI
RRFFKKKLKYEPKPDSSMLDTEGFGDGYKYAYPDFKRDVKRTDNLDELVQASGFSKKH
PGDRGLETAVESMRNAVYNSDDDMSNDEAEEENGEGDYSEEDEYYDSELDNESSEDDS
EDAQEEENERIIEALSSGVENLKMDKLGNYILE"
gene complement(<256788..>258155)
/gene="RTT106"
/locus_tag="YNL206C"
/db_xref="GeneID:855515"
mRNA complement(<256788..>258155)
/gene="RTT106"
/locus_tag="YNL206C"
/product="Rtt106p"
/transcript_id="NM_001183044.1"
/db_xref="GeneID:855515"
CDS complement(256788..258155)
/gene="RTT106"
/locus_tag="YNL206C"
/experiment="EXISTENCE:direct assay:GO:0003690
double-stranded DNA binding [PMID:20007951]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0042393 histone
binding [PMID:20007951|PMID:16157874|PMID:18662540]"
/experiment="EXISTENCE:genetic interaction:GO:0006335 DNA
replication-dependent chromatin assembly [PMID:18662540]"
/experiment="EXISTENCE:genetic interaction:GO:0006368
transcription elongation by RNA polymerase II
[PMID:18708354]"
/experiment="EXISTENCE:genetic interaction:GO:0031507
heterochromatin formation [PMID:17410207|PMID:20007951]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:19683497]"
/experiment="EXISTENCE:mutant phenotype:GO:0003690
double-stranded DNA binding [PMID:20007951]"
/experiment="EXISTENCE:mutant phenotype:GO:0006325
chromatin organization [PMID:18708354]"
/experiment="EXISTENCE:mutant phenotype:GO:0006368
transcription elongation by RNA polymerase II
[PMID:18708354]"
/experiment="EXISTENCE:mutant phenotype:GO:0031507
heterochromatin formation [PMID:17410207]"
/experiment="EXISTENCE:mutant phenotype:GO:0042393 histone
binding [PMID:20007951]"
/experiment="EXISTENCE:physical interaction:GO:0042393
histone binding [PMID:16157874]"
/note="Histone chaperone; involved in regulation of
chromatin structure in both transcribed and silenced
chromosomal regions; affects transcriptional elongation;
has a role in regulation of Ty1 transposition; interacts
physically and functionally with Chromatin Assembly
Factor-1 (CAF-1)"
/codon_start=1
/product="Rtt106p"
/protein_id="NP_014193.1"
/db_xref="GeneID:855515"
/db_xref="SGD:S000005150"
/translation="MSKLFLDELPESLSRKIGTVVRVLPSSLEIFEELYKYALNENSN
DRSGRHKKPRIDVSSDLLKTDEISETNTIFKLEGVSVLSPLRKKLDLVFYLSNVDGSP
VITLLKGNDRELSIYQLNKNIKMASFLPVPEKPNLIYLFMTYTSCEDNKFSEPVVMTL
NKENTLNQFKNLGLLDSNVTDFEKCVEYIRKQAILTGFKISNPFVNSTLVDTDAEKIN
SFHLQCHRGTKEGTLYFLPDHIIFGFKKPILLFDASDIESITYSSITRLTFNASLVTK
DGEKYEFSMIDQTEYAKIDDYVKRKQMKDKSMSEELKAKSKSKGQATDGTADQPSILQ
EATRQMQDEKKAGVFSDDDEENDQNFEAESDLSDGSGQESSDGAEDGEEAEEDDEEDD
EEEDKKGQSALNRDNSFASINGQPEQELQYKEFKEPLELEDIPIEIDNDDDEDDEDGS
GVEYD"
gene complement(<258375..>259277)
/gene="SPS18"
/locus_tag="YNL204C"
/gene_synonym="SPX18"
/db_xref="GeneID:855517"
mRNA complement(<258375..>259277)
/gene="SPS18"
/locus_tag="YNL204C"
/gene_synonym="SPX18"
/product="Sps18p"
/transcript_id="NM_001183042.1"
/db_xref="GeneID:855517"
CDS complement(258375..259277)
/gene="SPS18"
/locus_tag="YNL204C"
/gene_synonym="SPX18"
/experiment="EXISTENCE:mutant phenotype:GO:0030437
ascospore formation [PMID:7969036]"
/note="hypothetical protein, contains a putative
zinc-binding domain; expressed during sporulation; SPS18
has a paralog, GCS1, that arose from the whole genome
duplication"
/codon_start=1
/product="Sps18p"
/protein_id="NP_014195.1"
/db_xref="GeneID:855517"
/db_xref="SGD:S000005148"
/translation="MRLFENSKDMENRKRLLRAKKAAGNNNCFECKSVNPQFVSCSFG
IFICVNCANLLRGMGTNIFCVKSITMDNFEEKDVRRVEKSGNNRFGSFLSKNGILQNG
IPLREKYDNLFAKSYKRRLANEVRSNDINRNMYLGFNNFQQYTNGATSQIRDRTLREI
SNNSNASEGAEFVLPEKVLGSDNFQDCERFPACLSSERNLDENNVTSATSTLTIEKFQ
NDPIGTISRSWQLLSDALYKSYEDFKGSVVQPTIENIQQRNLPNDIKRSFVHFNEKLH
ETPHLPSPVFSCFTGGDILPPEFN"
gene <259578..>260456
/gene="SPS19"
/locus_tag="YNL202W"
/gene_synonym="SPX19"
/db_xref="GeneID:855518"
mRNA <259578..>260456
/gene="SPS19"
/locus_tag="YNL202W"
/gene_synonym="SPX19"
/product="2,4-dienoyl-CoA reductase (NADPH)"
/transcript_id="NM_001183040.1"
/db_xref="GeneID:855518"
CDS 259578..260456
/gene="SPS19"
/locus_tag="YNL202W"
/gene_synonym="SPX19"
/EC_number="1.3.1.124"
/experiment="EXISTENCE:direct assay:GO:0005782 peroxisomal
matrix [PMID:9268358]"
/experiment="EXISTENCE:direct assay:GO:0008670
2,4-dienoyl-CoA reductase (NADPH) activity [PMID:9268358]"
/experiment="EXISTENCE:direct assay:GO:0009062 fatty acid
catabolic process [PMID:9268358]"
/experiment="EXISTENCE:mutant phenotype:GO:0030437
ascospore formation [PMID:7969036]"
/note="Peroxisomal 2,4-dienoyl-CoA reductase; auxiliary
enzyme of fatty acid beta-oxidation; homodimeric enzyme
required for growth and sporulation on petroselineate
medium; expression induced during late sporulation and in
the presence of oleate"
/codon_start=1
/product="2,4-dienoyl-CoA reductase (NADPH)"
/protein_id="NP_014197.2"
/db_xref="GeneID:855518"
/db_xref="SGD:S000005146"
/translation="MNTANTLDGKFVTEGSWRPDLFKGKVAFVTGGAGTICRVQTEAL
VLLGCKAAIVGRDQERTEQAAKGISQLAKDKDAVLAIANVDVRNFEQVENAVKKTVEK
FGKIDFVIAGAAGNFVCDFANLSPNAFKSVVDIDLLGSFNTAKACLKELKKSKGSILF
VSATFHYYGVPFQGHVGAAKAGIDALAKNLAVELGPLGIRSNCIAPGAIDNTEGLKRL
AGKKYKEKALAKIPLQRLGSTRDIAESTVYIFSPAASYVTGTVLVVDGGMWHLGTYFG
HELYPEALIKSMTSKL"
gene complement(<260627..>263203)
/gene="PSY2"
/locus_tag="YNL201C"
/db_xref="GeneID:855520"
mRNA complement(<260627..>263203)
/gene="PSY2"
/locus_tag="YNL201C"
/product="Psy2p"
/transcript_id="NM_001183039.1"
/db_xref="GeneID:855520"
CDS complement(260627..263203)
/gene="PSY2"
/locus_tag="YNL201C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0019888 protein
phosphatase regulator activity [PMID:17517611]"
/experiment="EXISTENCE:direct assay:GO:0030289 protein
phosphatase 4 complex [PMID:16299494|PMID:17517611]"
/experiment="EXISTENCE:genetic interaction:GO:0006974 DNA
damage response [PMID:15598824]"
/experiment="EXISTENCE:mutant phenotype:GO:0030289 protein
phosphatase 4 complex [PMID:16299494]"
/experiment="EXISTENCE:mutant phenotype:GO:0051598 meiotic
recombination checkpoint signaling [PMID:20951350]"
/experiment="EXISTENCE:mutant phenotype:GO:1902660
negative regulation of glucose mediated signaling pathway
[PMID:24277933]"
/experiment="EXISTENCE:mutant phenotype:GO:2000002
negative regulation of DNA damage checkpoint
[PMID:16299494|PMID:17517611]"
/experiment="EXISTENCE:mutant phenotype:GO:2001034
positive regulation of double-strand break repair via
nonhomologous end joining [PMID:24498054]"
/note="Subunit of protein phosphatase PP4 complex; Pph3p
and Psy2p form the active complex, Psy4p may provide
additional substrate specificity; regulates recovery from
the DNA damage checkpoint, the gene conversion- and
single-strand annealing-mediated pathways of meiotic
double-strand break repair and efficient Non-Homologous
End-Joining (NHEJ) pathway; Pph3p and Psy2p localize to
foci on meiotic chromosomes; putative homolog of mammalian
R3"
/codon_start=1
/product="Psy2p"
/protein_id="NP_014198.1"
/db_xref="GeneID:855520"
/db_xref="SGD:S000005145"
/translation="MSLPGTPTTSPTPMDEDTEQAVSVNTEPKRVKVYILENNEWKDT
GTGFCIGEVDEGKFAYLVVSDEDSPTETLLKSKLEGNIEYQRQEETLIVWKDLGGKDI
ALSFEESMGCDTLCEFIVHVQRNIESNISLVTVKSSDNGLGSVHDIITGPVTLPSNDQ
QQNSQTLLEALKILNENTSFDFLKNETIEFILQSNYIDTLISHFHKAEEEKIPKDLFL
LSNIIKTLILYNKRDILESMVEDDRIMGIVGILEYDTEYPTSKANHRKYLGSKGPNFK
EVIPLENEDLKIIMKKCFRLQFLKDVVLVRFLDDHNFNLISEIVMDLETCIIDFLQVG
TFLDRLIELYDTKTLPESSSEKEKFVQKRKDGIRLLQQCVQMSINLDAVDRSKFYKTL
VRKGLFKVLDYAFHMETDSNVRILATDTIITIIEHDILLIHNVQNEDSFKRQHKSAPD
DKSSHRKYPQDYSSSTDSKLLLILSTILLSDRSPGLREQVVQALNTLLHPEGCVGNGE
GSYDLMGRSNYEAKNTSEDFPSFSYGLNSDSINLNNYHYSSDEMNNLEPESESEFQVM
EYFANFYNKIAPILFGPLIKKDITTEMAEIDGQIEKVTKDDLLLIHLVKLVSFVCTEH
DRVLSRRFILENGILDSVSKLIGGNHMMQLRLTAVRCIKNLMCLDDKYYHRYMISKNL
YAPVFKLFQENIDKNNLANSCIQDFFRIIITECRAYQSDGHNRKEKTNGSYDGNGNDV
KTNVNNNRTNFTILNKYLVQTYGDVLRKATDIPFIQDMLETGEENQPDHSSFENSIEG
GNDISVNMSTDGFASNHLEDIDIKNVKRLHSEIEHFENDPHYSGDQLAFKKSVDQMNA
ST"
gene complement(<263713..>264453)
/gene="NNR1"
/locus_tag="YNL200C"
/db_xref="GeneID:855521"
mRNA complement(<263713..>264453)
/gene="NNR1"
/locus_tag="YNL200C"
/product="NADHX epimerase"
/transcript_id="NM_001183038.1"
/db_xref="GeneID:855521"
CDS complement(263713..264453)
/gene="NNR1"
/locus_tag="YNL200C"
/EC_number="5.1.99.6"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0046496
nicotinamide nucleotide metabolic process [PMID:21994945]"
/experiment="EXISTENCE:direct assay:GO:0052856 NAD(P)HX
epimerase activity [PMID:21994945]"
/note="NADHX epimerase; catalyzes isomerization of (R)-
and (S)-NADHX; homologous to AIBP in mammals and the N-
terminal domain of YjeF in E.coli; enzyme is widespread in
eukaryotes, prokaryotes and archaea; the authentic,
non-tagged protein is detected in highly purified
mitochondria in high-throughput studies"
/codon_start=1
/product="NADHX epimerase"
/protein_id="NP_014199.1"
/db_xref="GeneID:855521"
/db_xref="SGD:S000005144"
/translation="MSTLKVVSSKLAAEIDKELMGPQIGFTLQQLMELAGFSVAQAVC
RQFPLRGKTETEKGKHVFVIAGPGNNGGDGLVCARHLKLFGYNPVVFYPKRSERTEFY
KQLVHQLNFFKVPVLSQDEGNWLEYLKPEKTLCIVDAIFGFSFKPPMREPFKGIVEEL
CKVQNIIPIVSVDVPTGWDVDKGPISQPSINPAVLVSLTVPKPCSSHIRENQTTHYVG
GRFIPRDFANKFGFEPFGYESTDQILKL"
gene complement(<264926..>266530)
/gene="GCR2"
/locus_tag="YNL199C"
/db_xref="GeneID:855522"
mRNA complement(<264926..>266530)
/gene="GCR2"
/locus_tag="YNL199C"
/product="Gcr2p"
/transcript_id="NM_001183037.1"
/db_xref="GeneID:855522"
CDS complement(264926..266530)
/gene="GCR2"
/locus_tag="YNL199C"
/experiment="EXISTENCE:direct assay:GO:0000122 negative
regulation of transcription by RNA polymerase II
[PMID:27190003]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:15817685]"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:15817685]"
/experiment="EXISTENCE:direct assay:GO:0060196 positive
regulation of antisense RNA transcription [PMID:27190003]"
/experiment="EXISTENCE:direct assay:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:11333224]"
/experiment="EXISTENCE:genetic interaction:GO:0003712
transcription coregulator activity [PMID:11333224]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:27190003]"
/experiment="EXISTENCE:mutant phenotype:GO:0006110
regulation of glycolytic process
[PMID:11333224|PMID:2247062]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:15789351|PMID:2247062|PMID:27190003|PMID:10455229|PM
ID:11333224|PMID:9335590]"
/experiment="EXISTENCE:mutant phenotype:GO:0060196
positive regulation of antisense RNA transcription
[PMID:27190003]"
/experiment="EXISTENCE:mutant phenotype:GO:0060963
positive regulation of ribosomal protein gene
transcription by RNA polymerase II [PMID:11333224]"
/note="Transcriptional activator of genes involved in
glycolysis; interacts and functions with the DNA-binding
protein Gcr1p"
/codon_start=1
/product="Gcr2p"
/protein_id="NP_014200.1"
/db_xref="GeneID:855522"
/db_xref="SGD:S000005143"
/translation="MHHQTKLDVFIIRAYNLLSNESVISGASLQSVTNSPQTTTNTPS
GMVNGAVGTGIANPTGLMGSDSTPNIDEIITSTGSNALTKTNSDSANGTPNGNSSSTS
AISNASNPATTGNNASSSATSNGIYTQAQYSQLFAKISKLYNATLSSGSIDDRSTSPK
SAIELYQRFQQMIKELELSFDASPYAKYFRRLDGRLWQIKTDSELENDELWRLVSMSI
FTVFDPQTGQILTQGRRKGNSLNTSTKGSPSDLQGINNGNNNGNNGNIGNGSNIKNYG
NKNMPNNRTKKRGTRVAKNAKNGKNNKNSNKERNGITDTSAFSNTTISNPGTNMLFDP
SLSQQLQKRLQTLSQDVNSRSLTGYYTQPTSPGSGGFEFGLSHADLNPNASSNTMGYN
TMSNNGSHSWKRRSLGSLDVNTLDDEAVEELLQLTNTSKRQRPMTTAAEGALINDGPD
TNLNANNTQMKVDLNPSNSMGPIDTEAVIRPLKEAYDAIISEKGQRIVQLERELELQR
QETQWLRKMLIEDMGCVRSMLRDLQR"
gene complement(<267608..>269593)
/gene="WHI3"
/locus_tag="YNL197C"
/db_xref="GeneID:855524"
mRNA complement(<267608..>269593)
/gene="WHI3"
/locus_tag="YNL197C"
/product="mRNA-binding protein WHI3"
/transcript_id="NM_001183035.1"
/db_xref="GeneID:855524"
CDS complement(267608..269593)
/gene="WHI3"
/locus_tag="YNL197C"
/experiment="EXISTENCE:direct assay:GO:0000932 P-body
[PMID:24386402]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:24386402|PMID:11691832]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11691832]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:35085498]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule
[PMID:24386402|PMID:24386330|PMID:26777405]"
/experiment="EXISTENCE:genetic interaction:GO:0007089
traversing start control point of mitotic cell cycle
[PMID:11691832]"
/experiment="EXISTENCE:mutant phenotype:GO:0001403
invasive growth in response to glucose limitation
[PMID:14685274]"
/experiment="EXISTENCE:mutant phenotype:GO:0003729 mRNA
binding [PMID:11691832]"
/experiment="EXISTENCE:mutant phenotype:GO:0007089
traversing start control point of mitotic cell cycle
[PMID:11691832]"
/experiment="EXISTENCE:mutant phenotype:GO:0007124
pseudohyphal growth [PMID:9055077|PMID:14685274]"
/experiment="EXISTENCE:mutant phenotype:GO:0008361
regulation of cell size [PMID:12089449]"
/experiment="EXISTENCE:mutant phenotype:GO:0061013
regulation of mRNA catabolic process [PMID:24386330]"
/experiment="EXISTENCE:mutant phenotype:GO:0061157 mRNA
destabilization [PMID:24386402]"
/note="Prion-like RNA binding protein that modulates mRNA
stability; regulates sister chromatid cohesion,
stress-response genes, critical cell size and ploidy;
controls CLN3 mRNA stability and translational efficiency;
colocalizes with P-bodies/stress granules to regulate
target mRNAs; Whi3p condensates and P-bodies regulate cell
fate, promoting senescence; self-templating mnemon that
forms inactive super-assemblies, preventing G1 arrest in
mother cells after unsuccessful mating and causing
sterility"
/codon_start=1
/product="mRNA-binding protein WHI3"
/protein_id="NP_014202.1"
/db_xref="GeneID:855524"
/db_xref="SGD:S000005141"
/translation="MQSSVYFDQTGSFASSSDNVVSSTTNTHNISPSHRSSLNLNTTS
HPHEASGRGSASGELYLNDTNSPLAISSMLNTLALGSMPQDIASSNISNHDNNIKGSY
SLKLSNVAKDITLRECYAIFALAEGVKSIELQKKNSSSSITSASLEDENDIFIIARFE
LLNLAINYAVILNSKNELFGPSFPNKTTVEIIDDTTKNLVSFPSSAIFNDTSRLNKSN
SGMKRPSLLSQRSRFSFSDPFSNDSPLSQQQSQQQQQQPQQPQQHSTQKHSPQQCNQQ
QVNSSIPLSSQGQVIGLHSNHSHQDLSVESTIQTSDIGKSFLLRDNTEINEKIWGTSG
IPSSINGYMSTPQPSTPTLEWGNTSASQHGSSFFLPSAASTAIAPTNSNTSANANASS
NNGASNNGANQALSASSQQPMMQIGNTINTSLTSSNSLPPYGLMSSQSQHISNMVNTS
DMNITPQKQNRFMQQPQPEHMYPVNQSNTPQKVPPARLSSSRNSHKNNSTTSLSSNIT
GSASISQADLSLLARIPPPANPADQNPPCNTLYVGNLPSDATEQELRQLFSGQEGFRR
LSFRNKNTTSNGHSHGPMCFVEFDDVSFATRALAELYGRQLPRSTVSSKGGIRLSFSK
NPLGVRGPNSRRGGSGNPNPNVNMLSSYNSNVGHIKN"
gene complement(<270275..>271171)
/gene="SLZ1"
/locus_tag="YNL196C"
/db_xref="GeneID:855525"
mRNA complement(<270275..>271171)
/gene="SLZ1"
/locus_tag="YNL196C"
/product="Slz1p"
/transcript_id="NM_001183034.1"
/db_xref="GeneID:855525"
CDS complement(270275..271171)
/gene="SLZ1"
/locus_tag="YNL196C"
/experiment="EXISTENCE:direct assay:GO:0036396 RNA
N6-methyladenosine methyltransferase complex
[PMID:22685417]"
/experiment="EXISTENCE:mutant phenotype:GO:0051321 meiotic
cell cycle [PMID:22685417]"
/experiment="EXISTENCE:mutant phenotype:GO:2000221
negative regulation of pseudohyphal growth
[PMID:22685417]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:22685417]"
/experiment="EXISTENCE:physical interaction:GO:0036396 RNA
N6-methyladenosine methyltransferase complex
[PMID:22685417]"
/note="Sporulation-specific protein with a leucine zipper
motif; subunit of the MIS complex which controls mRNA
methylation during the induction of sporulation;
homologous to human ZCH13H3, a subunit of the mammalian
m6A methyltransferase complex"
/codon_start=1
/product="Slz1p"
/protein_id="NP_014203.1"
/db_xref="GeneID:855525"
/db_xref="SGD:S000005140"
/translation="MMNKVDQIIGYKKYEVKLPKDRQVKKNKSKGGNVDQIDTKREKD
KMRAFGEERKKFLDKMAKNKKKNTSRKDREKPKEVEKENYKREDKRLKEQKKLSLAKE
FRFKEPNSEAINQNTAAENGKPKPQTGLDFDIDHQTVSKIMIDQAIQTSSPLNVQLTE
LFNDNVLDVSKDSQFVLQDMEFTSWERRWSNCSTTSNATTVSSVPDPKYNINYNDITS
FNSVALITEDLNISASSNGLHERGKKLLQQEMEYSNKVKNVTIGLERLCMDEKPVPDA
GITMQQASRWSEFPTCCDQAAI"
gene complement(<271523..>272308)
/locus_tag="YNL195C"
/db_xref="GeneID:855526"
mRNA complement(<271523..>272308)
/locus_tag="YNL195C"
/product="uncharacterized protein"
/transcript_id="NM_001183033.1"
/db_xref="GeneID:855526"
CDS complement(271523..272308)
/locus_tag="YNL195C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/note="hypothetical protein; shares a promoter with
YNL194C; the authentic, non-tagged protein is detected in
highly purified mitochondria in high-throughput studies;
YNL195C has a paralog, HBT1, that arose from the whole
genome duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014204.2"
/db_xref="GeneID:855526"
/db_xref="SGD:S000005139"
/translation="MLGLGQSAQAYASDDALNMNQAKDKTYSVPGCGRASDLKYPHRD
GHSSSHEQRSGILPTECPGPTLNTGAGSIGIPGCGKVTNRVVSDYNKNARSTLANFDS
SKMTEARMNSKNVPIGCQDTSDPHFNGPIDQHVPGAGSPQSQPHHIDAWNSVSSRRAD
NNNQDMMDPQTASSDRYNEKMMREENSGVSASSYTTKVQGYPASIPSFNQETEEKETY
AYGVGDRHNVPRNQIMDETNPSANVLNATDHSISHPENKVLHK"
gene complement(<272710..>273615)
/locus_tag="YNL194C"
/db_xref="GeneID:855527"
mRNA complement(<272710..>273615)
/locus_tag="YNL194C"
/product="uncharacterized protein"
/transcript_id="NM_001183032.1"
/db_xref="GeneID:855527"
CDS complement(272710..273615)
/locus_tag="YNL194C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836]"
/experiment="EXISTENCE:direct assay:GO:0005938 cell cortex
[PMID:11784867]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0030437
ascospore formation [PMID:11784867]"
/note="Integral membrane protein; required for sporulation
and plasma membrane sphingolipid content; similar to SUR7;
GFP-fusion protein is induced in response to the
DNA-damaging agent MMS; GFP-fusion protein is more
abundant at MCCs (membrane compartment occupied by Can1)
in the presence of glycerol and oleate; YNL194C has a
paralog, FMP45, that arose from the whole genome
duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014205.1"
/db_xref="GeneID:855527"
/db_xref="SGD:S000005138"
/translation="MSYKKFVYFINLFFLLGATLLTFFLILAGGRTTGVLKNFYWFQA
STSGFNSAPSVTRWYNYNWCGWESRGIAVNCSSKMAAQPFSPRDNFGSSPLMPSTFLN
NRNAYYYLSRVGWAMLLIGLFFLLITLVSVIASLIRYNRRTAALATAMSWITLFFITL
SACLYTGCYAKAVKAFHHENRDARLGPKNFGLIWTTVFLLIVNAICCTIMVATHKRNE
YIYDRSFASTKTVDSQTPTPVPTNGGIPSSVPVTEVQQSQSHQNHRFFKKLRTKKRTV
TSAGDEPDRVQEERVYTEQNVPVVS"
gene <274368..>276044
/locus_tag="YNL193W"
/db_xref="GeneID:855528"
mRNA <274368..>276044
/locus_tag="YNL193W"
/product="uncharacterized protein"
/transcript_id="NM_001183031.2"
/db_xref="GeneID:855528"
CDS 274368..276044
/locus_tag="YNL193W"
/note="hypothetical protein; exhibits a two-hybrid
interaction with Yhr151cp in a large-scale analysis"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014206.2"
/db_xref="GeneID:855528"
/db_xref="SGD:S000005137"
/translation="MGPPKNFKHFSKSNKHKKEQKALITQEDFYLAAIDCEEQADRWL
LSDIKKCLRFYLKALEHYENGLTALDSTQEGKYNIYYNETRLFLQIYTDYLANNGYIN
ILQYVKMDDMPDLSNLVLSLPQIIQRFEIVYETFPEQRTWDLQFNLLTCYLTLIESLD
DTVSPTVAMEGADILTLTNKYIEIFQHLVNYLLQELQNWSENAEQDSDDTDTELQRDT
LDEDAMQVTRDGSGIRTNGPVQPPAEVMDVSEQVTPSSLTEVLANSLKFNHALMELVI
ESKISIEKNVETKILNPIQINFLEDTTNKFYLQLRDIIDSISAAIPLDLKEIGLAKTL
IEGLNIISSGTFESLQDFVLQTVSFTDLLDEKDVQGKIDLSLIRVDIVEFAILCLNDY
SSDASWKLSGLLTKVLTEARTLLTDYRNQILFLKNQTLNEQLSHVVFQLCDVLVNSSD
NELRRYAIKESTEKSQKTPGGAHTLNILMKNANVFLNNAVAISSKQCGLQETIIDKLK
RNYIHNQAKERLLFLQRLEQKSNEDDGTSASPTAMTFDMPPEHPFYSHYR"
gene <276502..>279897
/gene="CHS1"
/locus_tag="YNL192W"
/gene_synonym="USA4"
/db_xref="GeneID:855529"
mRNA <276502..>279897
/gene="CHS1"
/locus_tag="YNL192W"
/gene_synonym="USA4"
/product="chitin synthase CHS1"
/transcript_id="NM_001183030.2"
/db_xref="GeneID:855529"
CDS 276502..279897
/gene="CHS1"
/locus_tag="YNL192W"
/gene_synonym="USA4"
/EC_number="2.4.1.16"
/experiment="EXISTENCE:direct assay:GO:0004100 chitin
synthase activity [PMID:7952171]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:8970154]"
/experiment="EXISTENCE:direct assay:GO:0045009 chitosome
[PMID:8970154]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0000920 septum
digestion after cytokinesis [PMID:2523889]"
/note="Chitin synthase I; requires activation from
zymogenic form in order to catalyze the transfer of
N-acetylglucosamine (GlcNAc) to chitin; required for
repairing the chitin septum during cytokinesis;
transcription activated by mating factor"
/codon_start=1
/product="chitin synthase CHS1"
/protein_id="NP_014207.2"
/db_xref="GeneID:855529"
/db_xref="SGD:S000005136"
/translation="MSDQNNRSRNEYHSNRKNEPSYELQNAHSGLFHSSNEELTNRNQ
RYTNQNASMGSFTPVQSLQFPEQSQQTNMLYNGDDGNNNTINDNERDIYGGFVNHHRQ
RPPPATAEYNDVFNTNSQQLPSEHQYNNVPSYPLPSINVIQTTPELIHNGSQTMATPI
ERPFFNENDYYYNNRNSRTSPSIASSSDGYADQEARPILEQPNNNMNSGNIPQYHDQP
FGYNNGYHGLQAKDYYDDPEGGYIDQRGDDYQINSYLGRNGEMVDPYDYENSLRHMTP
MERREYLHDDSRPVNDGKEELDSVKSGYSHRDLGEYDKDDFSRDDEYDDLNTIDKLQF
QANGVPASSSVSSIGSKESDIIVSNDNLTANRALKRSGTEIRKFKLWNGNFVFDSPIS
KTLLDQYATTTENANTLPNEFKFMRYQAVTCEPNQLAEKNFTVRQLKYLTPRETELML
VVTMYNEDHILLGRTLKGIMDNVKYMVKKKNSSTWGPDAWKKIVVCIISDGRSKINER
SLALLSSLGCYQDGFAKDEINEKKVAMHVYEHTTMINITNISESEVSLECNQGTVPIQ
LLFCLKEQNQKKINSHRWAFEGFAELLRPNIVTLLDAGTMPGKDSIYQLWREFRNPNV
GGACGEIRTDLGKRFVKLLNPLVASQNFEYKMSNILDKTTESNFGFITVLPGAFSAYR
FEAVRGQPLQKYFYGEIMENEGFHFFSSNMYLAEDRILCFEVVTKKNCNWILKYCRSS
YASTDVPERVPEFILQRRRWLNGSFFASVYSFCHFYRVWSSGHNIGRKLLLTVEFFYL
FFNTLISWFSLSSFFLVFRILTVSIALAYHSAFNVLSVIFLWLYGICTLSTFILSLGN
KPKSTEKFYVLTCVIFAVMMIYMIFCSIFMSVKSFQNILKNDTISFEGLITTEAFRDI
VISLGSTYCLYLISSIIYLQPWHMLTSFIQYILLSPSYINVLNIYAFCNVHDLSWGTK
GAMANPLGKINTTEDGTFKMEVLVSSSEIQANYDKYLKVLNDFDPKSESRPTEPSYDE
KKTGYYANVRSLVIIFWVITNFIIVAVVLETGGIADYIAMKSISTDDTLETAKKAEIP
LMTSKASIYFNVILWLVALSALIRFIGCSIYMIVRFFKKVTFR"
rep_origin 279982..280071
/note="ARS1414; ARS activated in mid-S phase"
/db_xref="SGD:S000077376"
gene <280432..>281505
/gene="DUG3"
/locus_tag="YNL191W"
/db_xref="GeneID:855530"
mRNA <280432..>281505
/gene="DUG3"
/locus_tag="YNL191W"
/product="glutamine amidotransferase subunit DUG3"
/transcript_id="NM_001183029.1"
/db_xref="GeneID:855530"
CDS 280432..281505
/gene="DUG3"
/locus_tag="YNL191W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:17179087]"
/experiment="EXISTENCE:direct assay:GO:0006751 glutathione
catabolic process [PMID:22277648]"
/experiment="EXISTENCE:direct assay:GO:0036374 glutathione
hydrolase activity [PMID:22277648]"
/experiment="EXISTENCE:direct assay:GO:0061672 glutathione
hydrolase complex [PMID:22277648]"
/experiment="EXISTENCE:genetic interaction:GO:0008233
peptidase activity [PMID:17179087]"
/experiment="EXISTENCE:genetic interaction:GO:0008242
omega peptidase activity [PMID:17179087]"
/experiment="EXISTENCE:mutant phenotype:GO:0006751
glutathione catabolic process
[PMID:17179087|PMID:22277648]"
/note="Component of glutamine amidotransferase (GATase
II); forms a complex with Dug2p to degrade glutathione
(GSH) and other peptides containing a gamma-glu-X bond in
an alternative pathway to GSH degradation by
gamma-glutamyl transpeptidase (Ecm38p)"
/codon_start=1
/product="glutamine amidotransferase subunit DUG3"
/protein_id="NP_014208.1"
/db_xref="GeneID:855530"
/db_xref="SGD:S000005135"
/translation="MCRFLIFKGKQPIRLSHLLTRPAHSIINQSFDSRLRLDRRRPMN
GDGFGVAYYPLDTELSEDGPCLFKAITPAWNNQNLSTLAEKTKSDLVFAHVRASTYGV
LSETNCHPFTYHSLCFMHNGGISNFKGIKRKLLNHIKDEYLNFIQGSTDSECAFALFL
DTLDKLGYDPKKQDGDFGNVALRKAMLRTIDYIRDWTKEANKDEAHVEPSLLNFAVTD
GSTVVVSRYITSKTDEAASLHFSCGSSFVETSPGEYRVERLDRNQDVIMVASEPLTFE
RGDWTAVPTNSILTIKKQTILLHPIIDEYYQEDPLYLRSSTLAESKGLMGSIPLAKAV
EKNVPPLEREGRTRPPTAVAHIA"
gene <282395..>283009
/locus_tag="YNL190W"
/db_xref="GeneID:855531"
mRNA <282395..>283009
/locus_tag="YNL190W"
/product="uncharacterized protein"
/transcript_id="NM_001183028.1"
/db_xref="GeneID:855531"
CDS 282395..283009
/locus_tag="YNL190W"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:10383953]"
/experiment="EXISTENCE:mutant phenotype:GO:0042631
cellular response to water deprivation [PMID:22442684]"
/note="Hydrophilin essential in desiccation-rehydration
process; cell wall protein; contains a putative
GPI-attachment site"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014209.1"
/db_xref="GeneID:855531"
/db_xref="SGD:S000005134"
/translation="MKFSSVTAITLATVATVATAKKGEHDFTTTLTLSSDGSLTTTTS
THTTHKYGKFNKTSKSKTPNHTGTHKYGKFNKTSKSKTPNHTGTHKYGKFNKTSKSKT
PNHTGTHKYGKFNKTSKSKTPNHTGTHKYGKFNKTSKSKTPNHTGTHKYGKFNKTKHD
TTTYGPGEKARKNNAAPGPSNFNSIKLFGVTAGSAAVAGALLLL"
gene <284260..>285888
/gene="SRP1"
/locus_tag="YNL189W"
/gene_synonym="KAP60; SCM1"
/db_xref="GeneID:855532"
mRNA <284260..>285888
/gene="SRP1"
/locus_tag="YNL189W"
/gene_synonym="KAP60; SCM1"
/product="karyopherin alpha"
/transcript_id="NM_001183027.1"
/db_xref="GeneID:855532"
CDS 284260..285888
/gene="SRP1"
/locus_tag="YNL189W"
/gene_synonym="KAP60; SCM1"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11914276|PMID:10725229]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:10725229]"
/experiment="EXISTENCE:direct assay:GO:0042564
NLS-dependent protein nuclear import complex
[PMID:19141610]"
/experiment="EXISTENCE:direct assay:GO:0044877
protein-containing complex binding [PMID:19046973]"
/experiment="EXISTENCE:direct assay:GO:0061608 nuclear
import signal receptor activity [PMID:7622450]"
/experiment="EXISTENCE:mutant phenotype:GO:0006607
NLS-bearing protein import into nucleus [PMID:19141610]"
/experiment="EXISTENCE:mutant phenotype:GO:0006612 protein
targeting to membrane [PMID:16929305]"
/experiment="EXISTENCE:mutant phenotype:GO:0031144
proteasome localization [PMID:25274630]"
/note="Karyopherin alpha homolog; forms a dimer with
karyopherin beta Kap95p to mediate import of nuclear
proteins, binds the nuclear localization signal of the
substrate during import; involved in cotranslational
protein degradation; binds ribosome-bound nascent
polypeptides; Srp1p and Sts1p couple proteasomes to
nascent polypeptides emerging from the ribosome for
cotranslational degradation"
/codon_start=1
/product="karyopherin alpha"
/protein_id="NP_014210.1"
/db_xref="GeneID:855532"
/db_xref="SGD:S000005133"
/translation="MDNGTDSSTSKFVPEYRRTNFKNKGRFSADELRRRRDTQQVELR
KAKRDEALAKRRNFIPPTDGADSDEEDESSVSADQQFYSQLQQELPQMTQQLNSDDMQ
EQLSATVKFRQILSREHRPPIDVVIQAGVVPRLVEFMRENQPEMLQLEAAWALTNIAS
GTSAQTKVVVDADAVPLFIQLLYTGSVEVKEQAIWALGNVAGDSTDYRDYVLQCNAME
PILGLFNSNKPSLIRTATWTLSNLCRGKKPQPDWSVVSQALPTLAKLIYSMDTETLVD
ACWAISYLSDGPQEAIQAVIDVRIPKRLVELLSHESTLVQTPALRAVGNIVTGNDLQT
QVVINAGVLPALRLLLSSPKENIKKEACWTISNITAGNTEQIQAVIDANLIPPLVKLL
EVAEYKTKKEACWAISNASSGGLQRPDIIRYLVSQGCIKPLCDLLEIADNRIIEVTLD
ALENILKMGEADKEARGLNINENADFIEKAGGMEKIFNCQQNENDKIYEKAYKIIETY
FGEEEDAVDETMAPQNAGNTFGFGSNVNQQFNFN"
gene <286308..>287609
/gene="KAR1"
/locus_tag="YNL188W"
/db_xref="GeneID:855533"
mRNA <286308..>287609
/gene="KAR1"
/locus_tag="YNL188W"
/product="Kar1p"
/transcript_id="NM_001183026.1"
/db_xref="GeneID:855533"
CDS 286308..287609
/gene="KAR1"
/locus_tag="YNL188W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005825 half bridge
of spindle pole body [PMID:10428957]"
/experiment="EXISTENCE:mutant phenotype:GO:0000742
karyogamy involved in conjugation with cellular fusion
[PMID:1607389]"
/experiment="EXISTENCE:mutant phenotype:GO:0030474 spindle
pole body duplication [PMID:3030557]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:8188750|PMID:10428957]"
/note="Protein involved in karyogamy and spindle pole body
duplication; involved in karyogamy during mating; involved
in centrosome separation and duplication during mitosis;
localizes to the half-bridge of the spindle pole body;
interacts with Spc72p during karyogamy; also interacts
with Cdc31p; essential gene"
/codon_start=1
/product="Kar1p"
/protein_id="NP_014211.1"
/db_xref="GeneID:855533"
/db_xref="SGD:S000005132"
/translation="MNVTSPKDGNHSFSKKNRFNTNKPRFHKLNEQAQSINLPEDRDS
IVSSNTTSIMTDDAFDYNEGIASRTKNINSDSDRSNDTIKQNNYNKRETGYNPFYNGS
GINQRYTQFRKREFEPTLAENKAEEYISDEDNVKIDEDNIENELQFTPKIKEASILRS
SLLGQRNVLNTRNPKSKESHIKVKPIINNKSSSQRKSSAALRKQLGKPLPLPYLNSPN
SDSTPTLQRKEEVFTDEVLQKKRELIESKWHRLLFHDKKMVEKKLESLREYERKRMPP
RGTDVSSSEQDNSFKISTPTKSYVSLEQKPLPNLSAMNNFNDVTDNKEKEETNNNILK
FQAQRDPLQILQSEIEMHTKKLDTIIELLKDDTDSKEKRKVVTNDNAAPEQMVNKGWR
KNVMMIYKKSGNIMKKYREYFLWTICILILLYCNIYVYYRF"
gene <287994..>289067
/gene="SWT21"
/locus_tag="YNL187W"
/db_xref="GeneID:855534"
mRNA <287994..>289067
/gene="SWT21"
/locus_tag="YNL187W"
/product="Swt21p"
/transcript_id="NM_001183025.1"
/db_xref="GeneID:855534"
CDS 287994..289067
/gene="SWT21"
/locus_tag="YNL187W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:19451230]"
/experiment="EXISTENCE:mutant phenotype:GO:0000245
spliceosomal complex assembly [PMID:40564906]"
/experiment="EXISTENCE:mutant phenotype:GO:0000398 mRNA
splicing, via spliceosome [PMID:19451230]"
/note="Spliceosome regulator; contributes to U1 snRNP
stability during early spliceosome assembly; contains a
consensus nuclear export signal (NES) sequence similar to
the consensus sequence recognized by Crm1p; interacts
genetically with Prp40p and Tgs1p; contains WD40 repeats"
/codon_start=1
/product="Swt21p"
/protein_id="NP_014212.1"
/db_xref="GeneID:855534"
/db_xref="SGD:S000005131"
/translation="MEKKVICQDIFWSCDGTSFVSVHNDFGIRQYLVPEESNTDKLNR
NLLLPFTRFFRNQSIVSCAIDPFYTLYNENSDRLAGDRIVVGGKNFPLQLYSLMDGQC
ILSYDTMNKINGEYETVYSVKIDVESRVYTGSCRNKVAIYDKSRRDAVWMNQSTKKAS
KGRQSIISCFEEQPMGGQALSRGSLLCGSYANEMFQVDCRHQRLERLNYTRTVAGGIV
QILTSDNGRYVYVVRRNSDAISIYDRRNLQHELNVLRLPFRIHHNSAKLKAYIDTAYG
LSMGTPQGTILNWGRDLVEFGGVPSHNSVEDPLITSIPPESEWRTNLDSTIPATVVKN
CPGDPELFALSHGGTISLCRFGG"
gene <289498..>291876
/gene="UBP10"
/locus_tag="YNL186W"
/gene_synonym="DOT4"
/db_xref="GeneID:855535"
mRNA <289498..>291876
/gene="UBP10"
/locus_tag="YNL186W"
/gene_synonym="DOT4"
/product="ubiquitin-specific protease UBP10"
/transcript_id="NM_001183024.2"
/db_xref="GeneID:855535"
CDS 289498..291876
/gene="UBP10"
/locus_tag="YNL186W"
/gene_synonym="DOT4"
/EC_number="3.4.19.12"
/experiment="EXISTENCE:direct assay:GO:0004843
cysteine-type deubiquitinase activity [PMID:10490600]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11914276|PMID:10490600]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:22902402]"
/experiment="EXISTENCE:direct assay:GO:0043596 nuclear
replication fork [PMID:31665643]"
/experiment="EXISTENCE:mutant phenotype:GO:0031509
subtelomeric heterochromatin formation
[PMID:26587833|PMID:15721261]"
/experiment="EXISTENCE:mutant phenotype:GO:0033567 DNA
replication, Okazaki fragment processing [PMID:39294185]"
/note="Ubiquitin-specific protease, deubiquitinates
Ub-protein moieties; interacts with proteins that function
in rRNA production and ribosome biogenesis via its
intrinsically disordered regions; stabilizes Rpa190p by
deubiquitination; controls PCNA deubiquitylation; may
regulate silencing by acting on Sir4p; involved in
posttranscriptionally regulating Gap1p, possibly other
transporters; localized to the nucleolus; null mutant
phenotypes are functionally complemented by human USP36"
/codon_start=1
/product="ubiquitin-specific protease UBP10"
/protein_id="NP_014213.2"
/db_xref="GeneID:855535"
/db_xref="SGD:S000005130"
/translation="MTTQESIKPLVDRILSNPLQFNAAMISNKSNNNDTSAAPENSSY
IVIGKQHNNNSNSTAIAATAESKQIKENNLIDRPNGKKTNTVPKSMAEALLLYTSKND
KDAADATGAKKSAELSTELSTEPPSSSSEDDKVGKEEEEEGEIFHEARDYVEPRKASL
KERDNADKGDGEDIGEDIGEDIGEDIGEDIGEDIGENLGSPLATIDDSSNENEKEKRK
ELSTSISSDDEIEDDEDEDDMDYDSSAMEKELPEEEENDSSSKISEGEKKSLYQDLME
NSTVEVNRYEPVNNTKENGNRNPKGEEEEEEEEELKHKSRSITPPVTISNLSNFYQFN
ENINDRGSLNSTRIVKNWGDKFTNLKPRGLLNHGVTCYTNAAVQAMLHIPSIQHYLFD
ILMGKYDSTISKNSVSYTLAETSKKMWLPVSKNPRKNVSASYINPKHLISRLDDINCM
MSEWQQEDSHEYFMSLMSRLQEDSVPKGHKLIESIIYDIFGGLLKQIVTCKSCGSISK
TEQPFYDLSLHLKGKKKLDPNSDLSSDSINGTSATTSTTTSNAATKPSLSSSSSVNLN
NGSPFAAASDLSSANRRFSIEKSIKDFFNPELIKVDKEQKGYVCEKCHKTTNAVKHSS
ILRAPETLLVHLKKFRFNGTSSSKMKQAVSYPMFLDLTEYCESKELPVKYQLLSVVVH
EGRSLSSGHYIAHCKQPDGSWATYDDEYINIISERDVLKEPNAYYLLYTRLTPKSVPL
PLAKSAMATGNVTSKSKQEQAVNEPNNRPLKINSKKNNRKKWKKNKKRKFTK"
gene complement(<292193..>292669)
/gene="MRPL19"
/locus_tag="YNL185C"
/gene_synonym="uL11m"
/db_xref="GeneID:855536"
mRNA complement(<292193..>292669)
/gene="MRPL19"
/locus_tag="YNL185C"
/gene_synonym="uL11m"
/product="mitochondrial 54S ribosomal protein uL11m
MRPL19"
/transcript_id="NM_001183023.1"
/db_xref="GeneID:855536"
CDS complement(292193..292669)
/gene="MRPL19"
/locus_tag="YNL185C"
/gene_synonym="uL11m"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:9151978]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:9151978]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005762
mitochondrial large ribosomal subunit [PMID:9151978]"
/note="Mitochondrial ribosomal protein of the large
subunit"
/codon_start=1
/product="mitochondrial 54S ribosomal protein uL11m
MRPL19"
/protein_id="NP_014214.1"
/db_xref="GeneID:855536"
/db_xref="SGD:S000005129"
/translation="MSQAAKNVIVKLIVGAGQAAPSPPVGPALGSKGIKAIDFCKEFN
ARSANYQPGVPVPVLITIKPDRTFTFEMKSPPTGYLLLKALKMDKGHGQPNVGTMLGS
APAKGPTRALGELSLKHVYEIAKIKKSDERHSLLEMEGIVKSIVGVAKSMGIKVVP"
gene complement(<292557..>292883)
/locus_tag="YNL184C"
/db_xref="GeneID:855537"
mRNA complement(<292557..>292883)
/locus_tag="YNL184C"
/product="uncharacterized protein"
/transcript_id="NM_001270758.1"
/db_xref="GeneID:855537"
CDS complement(292557..292883)
/locus_tag="YNL184C"
/note="hypothetical protein; expressed at both mRNA and
protein levels"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001257687.1"
/db_xref="GeneID:855537"
/db_xref="SGD:S000005128"
/translation="MKRVTLIVLPRRQFPFLKFHSKEALESAVNLQLIRRKKSVNIQI
DSVTFCIYFCLLYFRTLEYHRGTISLHNVTGSKKRDSKANSRSRPSGTITSRGARIGL
QGYKSH"
gene complement(<293137..>295509)
/gene="NPR1"
/locus_tag="YNL183C"
/db_xref="GeneID:855538"
mRNA complement(<293137..>295509)
/gene="NPR1"
/locus_tag="YNL183C"
/product="serine/threonine protein kinase NPR1"
/transcript_id="NM_001183021.1"
/db_xref="GeneID:855538"
CDS complement(293137..295509)
/gene="NPR1"
/locus_tag="YNL183C"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004674 protein
serine/threonine kinase activity [PMID:19780626]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:17095607]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:17095607]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:22118465]"
/experiment="EXISTENCE:mutant phenotype:GO:0045806
negative regulation of endocytosis [PMID:22118465]"
/experiment="EXISTENCE:mutant phenotype:GO:0090153
regulation of sphingolipid biosynthetic process
[PMID:23363605]"
/note="Protein kinase; stabilizes several plasma membrane
amino acid transporters by antagonizing their
ubiquitin-mediated degradation; phosphorylates Aly2p;
negatively regulates Ldb19p-mediated endocytosis by
phosphorylating Ldb19p; positively regulates activity of
the three Mep ammonium transport proteins; mediates
inhibitory phosphorylation of Mep2p and Par32p; TOR
complex negatively regulates Npr1p activity; NPR1 has a
paralog, PRR2, that arose from the whole genome
duplication"
/codon_start=1
/product="serine/threonine protein kinase NPR1"
/protein_id="NP_014216.1"
/db_xref="GeneID:855538"
/db_xref="SGD:S000005127"
/translation="MSSLTRLLQEKRKNETSNSSPRTSADTLTTTPESQSLDLHSRNK
SSSHIGSVSNSSSSDRNRANVPVPGSVTTVTQIYSEEDSSSTAGSSLDDRNQFSSSFL
NANFAHTASFYGTSAQSRDRFGSLINDQGTAGLSSHGGSFAAQNRITSRLSTTSHTSG
RAIPSLSSSIPYSVPNSNKDNNSSNSNSSSLSSSWLETYAGGMPNNISAIDSNVISSP
KVDSVEPRFVISKQKLQKASMDSNNANATQSRSISRSGSFSSQLGNFFFSKNSKESSN
SNSAGMSFSANSNGPSPNIKNPNVTNGSTPIPKPIRARQSSIYSASRQPTGSYTDNFY
GSPSSVHDHLPPSQSVPRSQHSSIGDLKRFFKKSSNSNLSSNSNNVIPNGSPLSSGIA
VPSHSHSSSHFAAGNNSYSTSYNGNGDTIYSHSHGGSGIPFSKRYIKTGADLGAGAGG
SVKLAQRISDNKIFAVKEFRTKFENESKRDYVKKITSEYCIGTTLNHPNIIETIEIVY
ENDRILQVMEYCEYDLFAIVMSNKMSYEEICCCFKQILTGVQYLHSIGLAHRDLKLDN
CVINEKGIVKLIDFGAAVVFSYPFSKNLVEASGIVGSDPYLAPEVCIFAKYDPRPVDI
WSSAIIFACMILKKFPWKIPKLRDNSFKLFCSGRDCDSLSSLVTRTPDPPSYDESHST
EKKKPESSSNNVSDPNNVNIGPQRLLHSLPEETQHIVGRMIDLAPACRGNIEEIMEDP
WIRSIDMCHLVEDGLSFKVVRGEDHHHTQVDQSEAHIAGLEKKKKKQNNQ"
gene complement(<295960..>297627)
/gene="IPI3"
/locus_tag="YNL182C"
/db_xref="GeneID:855539"
mRNA complement(<295960..>297627)
/gene="IPI3"
/locus_tag="YNL182C"
/product="chromatin-binding/pre-rRNA-processing protein
IPI3"
/transcript_id="NM_001183020.1"
/db_xref="GeneID:855539"
CDS complement(295960..297627)
/gene="IPI3"
/locus_tag="YNL182C"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:22421151]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14690591]"
/experiment="EXISTENCE:direct assay:GO:0005654 nucleoplasm
[PMID:15528184]"
/experiment="EXISTENCE:direct assay:GO:0005656 nuclear
pre-replicative complex [PMID:22421151]"
/experiment="EXISTENCE:direct assay:GO:0120330 rixosome
complex [PMID:14759368]"
/experiment="EXISTENCE:mutant phenotype:GO:0000027
ribosomal large subunit assembly [PMID:15528184]"
/experiment="EXISTENCE:mutant phenotype:GO:0006267
pre-replicative complex assembly involved in nuclear cell
cycle DNA replication [PMID:22421151]"
/experiment="EXISTENCE:mutant phenotype:GO:0006364 rRNA
processing [PMID:14759368]"
/experiment="EXISTENCE:mutant phenotype:GO:0030174
regulation of DNA-templated DNA replication initiation
[PMID:22421151]"
/note="Component of the Rix1 complex and pre-replicative
complexes (pre-RCs); required for processing of ITS2
sequences from 35S pre-rRNA; component of the pre-60S
ribosomal particle with the dynein-related AAA-type ATPase
Mdn1p; required for pre-RC formation and maintenance
during DNA replication licensing; highly conserved protein
which contains several WD40 motifs; IPI3 is an essential
gene; other members include Rix1p, Ipi1p, and Ipi3p"
/codon_start=1
/product="chromatin-binding/pre-rRNA-processing protein
IPI3"
/protein_id="NP_014217.1"
/db_xref="GeneID:855539"
/db_xref="SGD:S000005126"
/translation="MDEQVIFTTNTSGTIASVHSFEQINLRQCSTQSRNSCVQVGNKY
LFIAQAQKALINVYNLSGSFKRESVEQRLPLPEILKCLEVVENDGVQYDRIQGVNHNL
PDFNLPYLLLGSTESGKLYIWELNSGILLNVKPMAHYQSITKIKSILNGKYIITSGND
SRVIIWQTVDLVSASNDDPKPLCILHDHTLPVTDFQVSSSQGKFLSCTDTKLFTVSQD
ATIRCYDLSLIGSKKKQKANENDVSIGKTPVLLATFTTPYSIKSIVLDPADRACYIGT
AEGCFSLNLFYKLKGNAIVNLLQSAGVNTVQKGRVFSLVQRNSLTGGENEDLDALYAM
GQLVCENVLNSNVSCLEISMDGTLLLIGDTEGKVSIAEIYSKQIIRTIQTLTTSQDSV
GEVTNLLTNPYRLERGNLLFEGESKGKQPSNNNGHNFMKIPNLQRVIFDGKNKGHLHD
IWYQIGEPEAETDPNLALPLNDFNAYLEQVKTQESIFSHIGKVSSNVKVIDNKIDATS
SLDSNAAKDEEITELKTNIEALTHAYKELRDMHEKLYEEHQQMLDKQ"
gene <298335..>299558
/gene="PBR1"
/locus_tag="YNL181W"
/db_xref="GeneID:855540"
mRNA <298335..>299558
/gene="PBR1"
/locus_tag="YNL181W"
/product="putative oxidoreductase"
/transcript_id="NM_001183019.2"
/db_xref="GeneID:855540"
CDS 298335..299558
/gene="PBR1"
/locus_tag="YNL181W"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:14690591]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762|PMID:14562095|PMID:14690591]"
/note="Putative oxidoreductase; required for cell
viability"
/codon_start=1
/product="putative oxidoreductase"
/protein_id="NP_014218.2"
/db_xref="GeneID:855540"
/db_xref="SGD:S000005125"
/translation="MPLNIIGTALLDGTDKIPYYQTIKKVAPYVLATGAIKYWSRGPS
NTWERKLHGKVYLVTGATSQGMGTSVAYKMAELGAQLIILTREVDEWVTEWCEELREK
TKNELIFVEKCDLSNLWEIRKFATSWLDNSPPRRLDGVIVMSGDMEPWGIPKISLPQR
RSSKDGLELQIATNYVAIFHLLNLLQPSFKAQPPDRDVRIILATCWLQVVGDINIEDP
LWQNAKYKSALKFFASSKLQLGLSMMELQRRLTEDIKNQKTNGAERTGKNVTITMVQP
GTMRSNSLRRVISNGSVVLLIILYCILLYPILWLFTKSGRRGDQSFLYALMTPELEEV
NLKDTKVKYISDCSIVKFARKEFDDEELQKKLFDNTERDILQLEKKVAAKRNANKTGN
QNSKKKSQNKSRKDD"
gene complement(<299654..>300649)
/gene="RHO5"
/locus_tag="YNL180C"
/gene_synonym="YNS0"
/db_xref="GeneID:855541"
mRNA complement(<299654..>300649)
/gene="RHO5"
/locus_tag="YNL180C"
/gene_synonym="YNS0"
/product="Rho family GTPase RHO5"
/transcript_id="NM_001183018.1"
/db_xref="GeneID:855541"
CDS complement(299654..300649)
/gene="RHO5"
/locus_tag="YNL180C"
/gene_synonym="YNS0"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:18216266]"
/experiment="EXISTENCE:direct assay:GO:0003924 GTPase
activity [PMID:11591390]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:18216266]"
/experiment="EXISTENCE:direct assay:GO:0012505
endomembrane system [PMID:18216266]"
/experiment="EXISTENCE:genetic interaction:GO:0106071
positive regulation of adenylate cyclase-activating G
protein-coupled receptor signaling pathway
[PMID:40924759]"
/experiment="EXISTENCE:mutant phenotype:GO:0003924 GTPase
activity [PMID:40924759]"
/experiment="EXISTENCE:mutant phenotype:GO:0006915
apoptotic process [PMID:18216266]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress [PMID:18216266]"
/experiment="EXISTENCE:mutant phenotype:GO:0106071
positive regulation of adenylate cyclase-activating G
protein-coupled receptor signaling pathway
[PMID:40924759]"
/note="Non-essential small GTPase of the Rho/Rac family of
Ras-like proteins; likely involved in protein kinase C
(Pkc1p)-dependent signal transduction pathway that
controls cell integrity; role in carbohydrate metabolism,
acting upstream of the Ras2p GTPase in cAMP-mediated
protein kinase A signaling; regulated by phosphorylation
and ubiquitination; necessary for oxidant and ramped heat
stress-induced cell death; ortholog of mammalian RAC1"
/codon_start=1
/product="Rho family GTPase RHO5"
/protein_id="NP_014219.1"
/db_xref="GeneID:855541"
/db_xref="SGD:S000005124"
/translation="MRSIKCVIIGDGAVGKTSLLISYTTNSFPTDYVPTVFDNYSTTI
AIPNGTASSPLELDNGNDKRGSLSSASSSPSTDRKLYKINLWDTAGQEDYDRLRPLCY
PQTDIFLICFSVSEHASFANVTEKWLPELKQTSNIEGTSLYTKLGKYPILLVGTKSDL
RDDPATQKKLQEANSDYVSQEEIDELVQRCGFMGYTECSAATQAGVREVFEQAVRYAI
YEPESPNQKSANHTLTDELTTATTNTNGDKNIREQKQQPHHNNSTDSTLPKGSLQQEK
EALNIKPTKKGQKDKIHEQSKSKGSKIASNNHHNKQAKPKTRNDKKKKKSKCVIL"
gene <302680..>303402
/gene="RPS3"
/locus_tag="YNL178W"
/gene_synonym="SUF14"
/db_xref="GeneID:855543"
mRNA <302680..>303402
/gene="RPS3"
/locus_tag="YNL178W"
/gene_synonym="SUF14"
/product="40S ribosomal protein uS3 RPS3"
/transcript_id="NM_001183016.3"
/db_xref="GeneID:855543"
CDS 302680..303402
/gene="RPS3"
/locus_tag="YNL178W"
/gene_synonym="SUF14"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:18782943]"
/experiment="EXISTENCE:direct assay:GO:0003906
DNA-(apurinic or apyrimidinic site) endonuclease activity
[PMID:11561734]"
/experiment="EXISTENCE:direct assay:GO:0022627 cytosolic
small ribosomal subunit
[PMID:18782943|PMID:385049|PMID:3533916]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/experiment="EXISTENCE:direct assay:GO:0030688
preribosome, small subunit precursor [PMID:16738661]"
/experiment="EXISTENCE:genetic interaction:GO:0000056
ribosomal small subunit export from nucleus
[PMID:22570489]"
/experiment="EXISTENCE:mutant phenotype:GO:0000054
ribosomal subunit export from nucleus [PMID:16246728]"
/experiment="EXISTENCE:mutant phenotype:GO:0002181
cytoplasmic translation [PMID:28223523]"
/experiment="EXISTENCE:mutant phenotype:GO:0070651
nonfunctional rRNA decay [PMID:28956756]"
/experiment="EXISTENCE:mutant phenotype:GO:1990145
maintenance of translational fidelity [PMID:30465652]"
/note="Protein component of the small (40S) ribosomal
subunit; has apurinic/apyrimidinic (AP) endonuclease
activity; essential for viability; nascent Rps3p is bound
by specific chaperone Yar1p during translation; homologous
to mammalian ribosomal protein S3 and bacterial S3"
/codon_start=1
/product="40S ribosomal protein uS3 RPS3"
/protein_id="NP_014221.3"
/db_xref="GeneID:855543"
/db_xref="SGD:S000005122"
/translation="MVALISKKRKLVADGVFYAELNEFFTRELAEEGYSGVEVRVTPT
KTEVIIRATRTQDVLGENGRRINELTLLVQKRFKYAPGTIVLYAERVQDRGLSAVAQA
ESMKFKLLNGLAIRRAAYGVVRYVMESGAKGCEVVVSGKLRAARAKAMKFADGFLIHS
GQPVNDFIDTATRHVLMRQGVLGIKVKIMRDPAKSRTGPKALPDAVTIIEPKEEEPIL
APSVKDYRPAEETEAQAEPVEA"
gene complement(<303686..>304615)
/gene="MRPL22"
/locus_tag="YNL177C"
/gene_synonym="uL22m"
/db_xref="GeneID:855544"
mRNA complement(<303686..>304615)
/gene="MRPL22"
/locus_tag="YNL177C"
/gene_synonym="uL22m"
/product="mitochondrial 54S ribosomal protein uL22m
MRPL22"
/transcript_id="NM_001183015.2"
/db_xref="GeneID:855544"
CDS complement(303686..304615)
/gene="MRPL22"
/locus_tag="YNL177C"
/gene_synonym="uL22m"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:12392552]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:12392552]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005762
mitochondrial large ribosomal subunit [PMID:12392552]"
/note="Mitochondrial ribosomal protein of the large
subunit"
/codon_start=1
/product="mitochondrial 54S ribosomal protein uL22m
MRPL22"
/protein_id="NP_014222.2"
/db_xref="GeneID:855544"
/db_xref="SGD:S000005121"
/translation="MNFHTARISQVGVISRALLSSVSRRWIHVTPISLNNSGGSLFGS
ITENKPKEGKNRGDEDAGSFSNRLAIASDSSGEAPEVNRDSITIENDKLLQQHIISLQ
QPEQLASQSLLSPLKREIYEANCKINGGFYKKDTIVKLPNSSERYKLKLTKREIEVLE
PSVYAQSYRIKSSMKKATLLLRLLGGLDVMKAISQCHFSNKKIAREVAELLQKGVKDG
QKLGLKPEDLYISQIWTGSDGFWRKRVEFKARTRIGIISHPYIHVRCILRTKSVTKRR
LAYEAHLKEQKRAPWVQLGDKPIRGVTGGVYKW"
gene complement(<305070..>306980)
/gene="TDA7"
/locus_tag="YNL176C"
/db_xref="GeneID:855545"
mRNA complement(<305070..>306980)
/gene="TDA7"
/locus_tag="YNL176C"
/product="Tda7p"
/transcript_id="NM_001183014.2"
/db_xref="GeneID:855545"
CDS complement(305070..306980)
/gene="TDA7"
/locus_tag="YNL176C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:14562095|PMID:23708375]"
/note="Cell cycle-regulated gene of unknown function;
promoter bound by Fkh2p; null mutant is sensitive to
expression of the top1-T722A allele; TDA7 has a paralog,
YDL211C, that arose from the whole genome duplication"
/codon_start=1
/product="Tda7p"
/protein_id="NP_014223.2"
/db_xref="GeneID:855545"
/db_xref="SGD:S000005120"
/translation="MNSNSTIGRTTLGESDTISLSFSEPSSSLNSRSTDVVFASTSTL
VPQQGSLTSLPPVSSTATPTYYSTSLTYDETLHTSIDVSSTSTLVSSTDSSSSSEQDT
YSSQYDPATSSYSIITPSMSIFSSTSPMSSSSSITSEWSSLTSTTPTLSSSATSLSSS
WSSLSSPSSLLVSSSLSLSLSSSYSDTKLFSFDSRSSIFSPSTPTVISPSYTYLSSIS
ATSFQISTTSELSSSWFSTISSPSTISNKDTTFPSSSRNTSTSFYSSSLSSTNDFSTI
SKSSKLSPSASSSTVSISTISVPTSSSVSSSSSKVPSNRPSSSSSSDDTTSAYSSTYT
FQSLQSTTSSSIPPTTQTPSTSTISTSPIPTSSQVFNTVAISSSEDSKTIYYFYTQTY
DITDSSTTFVTGLPTTIAVAKSEVTSFSAPSSTITADMSFYQHWLDGSLDNNKNQGTS
KTNTGTIVGSVVGSVGGILICVLVVWFMLVRKRKAKRHFKENDSFCHEIGRRTGFPTT
AQAKEASLQAQDSGSQQRNTETASANNPFSNEFNFKARGNPPPVPPPRNVTAMNGSFQ
NMRSNFMDQENRFSYGSSFTYSSLGSSTQGGFSTLSSNSIRLGRGLDNDISHDERNTV
QNNSQGFLREII"
gene complement(<307401..>308612)
/gene="NOP13"
/locus_tag="YNL175C"
/db_xref="GeneID:855547"
mRNA complement(<307401..>308612)
/gene="NOP13"
/locus_tag="YNL175C"
/product="Nop13p"
/transcript_id="NM_001183013.2"
/db_xref="GeneID:855547"
CDS complement(307401..308612)
/gene="NOP13"
/locus_tag="YNL175C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:22842922|PMID:11452019]"
/experiment="EXISTENCE:direct assay:GO:0030684 preribosome
[PMID:17922018]"
/note="Nucleolar protein found in preribosomal complexes;
contains an RNA recognition motif (RRM); relative
distribution to the nucleolus increases upon DNA
replication stress"
/codon_start=1
/product="Nop13p"
/protein_id="NP_014224.2"
/db_xref="GeneID:855547"
/db_xref="SGD:S000005119"
/translation="MSETELSKEDAVTKKDNEEQVKKALLDPTKKRKAEDEIEIDLKS
SIPLSKKQKRLLRRGKVTLEELNAKYNIDPKSIEEYKEDAEKKKSGASEKDAQGEEST
INTPTGDESGEVVKKKKKDENKYGVWIGNLSFDTTKDDLVRFFIAKTKDNEDEKSRVT
EQDITRLSMPRVAAKNSNAMKNKGFCYMFFKNVEQMKAVLELSESHLNGRNMLIKDSE
NYSGRPDKDDLVAMSKNPPSRILFVGNLSFDVTDDLLRKHFQHCGDIVKIRMATFEDS
GKCKGFAFIDFKNEEGSTNALKDKSCRKIAGRPLRMEYGEDRSKRQVRKKVENVSRNN
SSSFDISNNKGYDRAGQDNGSKPEYKRSNANRRPPVDSNNRTKSSVALATAQRGSAAI
VPSQGKKVKFD"
gene complement(<308957..>310057)
/gene="MDG1"
/locus_tag="YNL173C"
/db_xref="GeneID:855548"
mRNA complement(<308957..>310057)
/gene="MDG1"
/locus_tag="YNL173C"
/product="Mdg1p"
/transcript_id="NM_001183011.1"
/db_xref="GeneID:855548"
CDS complement(308957..310057)
/gene="MDG1"
/locus_tag="YNL173C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:8914522|PMID:22842922]"
/experiment="EXISTENCE:mutant phenotype:GO:0000750
pheromone-dependent signal transduction involved in
conjugation with cellular fusion [PMID:8914522]"
/note="Plasma membrane protein; involved in G-protein
mediated pheromone signaling pathway; overproduction
suppresses bem1 mutations; MDG1 has a paralog, CRP1, that
arose from the whole genome duplication"
/codon_start=1
/product="Mdg1p"
/protein_id="NP_014226.1"
/db_xref="GeneID:855548"
/db_xref="SGD:S000005117"
/translation="MQSSLPQFTFKWPKGPEAIILTGTFDDWKGTLPMVKDPSGAFEI
TLPVTFDSPSSKFYFKFIVDGQWLPSKDYKVNIDEGVENNFITEEDVIKQRENGSSTL
VPESAGLAVSKNAPLIEPEAEKRAKKLRKFKIKRVIKTNKQTGERSIFSQEVVELPDS
EDETQQVNKTGKNADGLSGTTTIIENNVGVNEEKAIKPYEENHPKVNLVKSEGYVTDG
LGKTQSSESRLYELSAEDLEKEEEEEDEDKGGGKDTSTSADAEASEDQNKEPLSKSAK
FEKPEEKVPVSSITSHAKETSVKPTGKVATETQTYETKQGAPTAAAKKIEAKKATRPS
KPKGTKETPNKGVQKNPAKNGGFFKKLAQLLK"
gene <310636..>315882
/gene="APC1"
/locus_tag="YNL172W"
/db_xref="GeneID:855549"
mRNA <310636..>315882
/gene="APC1"
/locus_tag="YNL172W"
/product="anaphase promoting complex subunit 1"
/transcript_id="NM_001183010.4"
/db_xref="GeneID:855549"
CDS 310636..315882
/gene="APC1"
/locus_tag="YNL172W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005680
anaphase-promoting complex [PMID:9469814]"
/experiment="EXISTENCE:direct assay:GO:0060090 molecular
adaptor activity [PMID:16481473]"
/experiment="EXISTENCE:mutant phenotype:GO:0007091
metaphase/anaphase transition of mitotic cell cycle
[PMID:8895471]"
/experiment="EXISTENCE:mutant phenotype:GO:0010458 exit
from mitosis [PMID:8895471]"
/experiment="EXISTENCE:mutant phenotype:GO:0016567 protein
ubiquitination [PMID:16481473]"
/experiment="EXISTENCE:mutant phenotype:GO:0031145
anaphase-promoting complex-dependent catabolic process
[PMID:8895471|PMID:16481473]"
/experiment="EXISTENCE:mutant phenotype:GO:0061630
ubiquitin protein ligase activity [PMID:16481473]"
/note="Largest subunit of the Anaphase-Promoting
Complex/Cyclosome; APC/C is a ubiquitin-protein ligase
required for degradation of anaphase inhibitors, including
mitotic cyclins, during the metaphase/anaphase transition;
component of the platform domain of the APC/C, based on
structural analysis; localizes to nuclear foci that become
diffuse upon DNA replication stress"
/codon_start=1
/product="anaphase promoting complex subunit 1"
/protein_id="NP_014227.4"
/db_xref="GeneID:855549"
/db_xref="SGD:S000005116"
/translation="MTSKPSTRNDYLPRETHNGEYTGDSPEWQLQINITNKIGGINGD
IWLSRDGRSVKWCIEDQCLRQFTYNQKIIKAGYIDFEKTPDCFVVVLSDIAHVYMLKN
GGSTTVCFPFQIGNAFWYANGVILERETSASFMDGGYDLKPIEFDLKHKYITLTDPMA
PFGLISITNTFKGGINSASGNKTDILQDFQLVLFPSDKDKCIAVFLDRNSKVLRFYYS
RILSSDQSRKGELTISSTKKTGLDAAGNSQKSGGISKDLRKFSHLTRRSTSINSNSHD
FNAAERVLSGNVGNASGRTDIFALPSSCSRRSLSATLDRMGNNIAPTNRAAPSGFFDS
SSANTATHSNITPVSQPMQQQQQEYLNQTATSSKDIVLTEISSLKLPDDIIFTSRRLS
SILSKLKFLSLRFERREGLLIFHEPTHFCKIWLIDLLPDVLDSIPFKIYGNSPQNMIR
LENLKLKEPSRIQAMYIHELLESCLILVSEGQNKEEYKACLYDPFVKITSPSKNISEE
LTKQNSLPSLQKLFPYPETSFTKLCFEAVKYITSPAFNISFIFLWQSAYSILLSRAND
DVVGGLKMEHDAFSLVLSLLILPIPSSSAQEYQEYKEIYERDLFQHLKQDSEITSSVL
PRIVIGLHLIREEYSLNVLCRNEHALLGQFLRFATAAMGWPDLWQSYYVPKMDSESKT
FLHPREQNSTFFHPLDEPPSITKSLYSITENSSIPLCPFISFSRLVATDTQVELRITP
RSFKILGLYELVHSPNFLPDYVLGILSSFKVDKDELQTYPLGILVPLQNILKILEDKL
SEVRDNLELLDRADLQRCSAIINSIRSDSKEVLKRGQRDSYMLCKVPLAKNRSSLSKK
PSDIYSILSEIVKSASQVPLDGSAMRMSNIQDDEDIDEGRSLKLNAGLIFSEDKRFTH
VVSLLAYYRPTKTQFFTTKTEYAQILAQKKYFAKIMALRTCTNGVGWGAVAYATEKPI
STQKWVIQPLNLISVFPDDTKITVKAPEDIAHDIVEWGQFHAGVSSGLRISKKATGIT
GSWIAFNKPKELDAYHGGFLLGLGLNGHLKNLEEWHIYNYLSPRNTHISIGLLLGMSS
SMKGSMDSKLIKVISVHLVAFLPSGSSDLNIDLKLQTAGIIGMGMLYLNSRHKRMSDS
IFAQLVSLLNVNDEMVADEEYRLAAGISLGLINLGAGQTKLRKWDSSLLGLGDDLPED
VYDSSDVEQNVMYEDLTTKLLEIVTSTYDVENDWIPENSQIGAVIAIMFLFLKSNNFG
ISNMLKVDLKEILKANINTRPELLMYREWASNMILWEFIGDDLSFIMKDVDIGVKFSE
LNTDLLPIYYTMAGRILAMGIRFASTGNLKIRNILLSLVDKFLPLYQYPGKQNLDFRL
TISVINVLTNVIVVSLSMVMCASGDLEVLRRVKYLHEVASGPYSDLFQEIPSSKSDVS
GVTQVTSNTNTPGNSDRERVDETAASLDDERSSNGSDISDPTAYLEDKKDIDDHYGKF
ISTNLALGFLFLGSGQYALNTSTLESIAFLSMSVLPTYTTPHPLQELKHFWSMAVEPR
CLVIKDISTGDAVNNVPIELVVEEDVEKEEVIREISTPCLLPDFSKIKSIRVKMHGYF
PLEVNFTKDYSASDFFSGGTIIYIQRKSESVFENKASFRNVEDIHVALKRKAAESKNY
SRLNLKNEQGNTTSSQLVESLGIQDLTMVELDTLLSAGNNTALTDSESYNLGLLCSDK
NSGDILDCQLELWYKSFGPHDE"
gene complement(<316169..>317671)
/gene="PSD1"
/locus_tag="YNL169C"
/db_xref="GeneID:855552"
mRNA complement(<316169..>317671)
/gene="PSD1"
/locus_tag="YNL169C"
/product="phosphatidylserine decarboxylase 1"
/transcript_id="NM_001183007.1"
/db_xref="GeneID:855552"
CDS complement(316169..317671)
/gene="PSD1"
/locus_tag="YNL169C"
/EC_number="4.1.1.65"
/experiment="EXISTENCE:direct assay:GO:0004609
phosphatidylserine decarboxylase activity [PMID:23124206]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:23124206]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:34818062]"
/experiment="EXISTENCE:direct assay:GO:0006656
phosphatidylcholine biosynthetic process [PMID:6427211]"
/experiment="EXISTENCE:direct assay:GO:0016540 protein
autoprocessing [PMID:25829489]"
/experiment="EXISTENCE:direct assay:GO:0140042 lipid
droplet formation [PMID:34818062]"
/experiment="EXISTENCE:mutant phenotype:GO:0004609
phosphatidylserine decarboxylase activity [PMID:8407984]"
/experiment="EXISTENCE:mutant phenotype:GO:0006122
mitochondrial electron transport, ubiquinol to cytochrome
c [PMID:30926815]"
/experiment="EXISTENCE:mutant phenotype:GO:0010636
positive regulation of mitochondrial fusion
[PMID:23045528]"
/experiment="EXISTENCE:mutant phenotype:GO:0010954
positive regulation of protein processing [PMID:23045528]"
/note="Phosphatidylserine decarboxylase of the
mitochondrial inner membrane; converts phosphatidylserine
to phosphatidylethanolamine; regulates mitochondrial
fusion and morphology; partly exposed to the mitochondrial
intermembrane space; ER-localized fraction is involved in
formation of lipid droplets; autocatalytically processed"
/codon_start=1
/product="phosphatidylserine decarboxylase 1"
/protein_id="NP_014230.1"
/db_xref="GeneID:855552"
/db_xref="SGD:S000005113"
/translation="MSIMPVKNALAQGRTLLMGRMPAVKFSTRMQLRNRTAVLWNRKF
STRLFVQQRRSSGEIVDRAKAAAANSGRKQVSMKWVVLTSFTIVLGTILLVSRNDSTE
EDATEGKKGRRTRKIKIFNNNWLFFCYSTLPLNAMSRLWGQVNSLTLPIWVRPWGYRL
YSFLFGVNLDEMEDPDLTHYANLSEFFYRNIKPGTRPVAQGEDVIASPSDGKILQVGI
INSETGEIEQVKGMTYSIKEFLGTHSHPLMSKSASSLDLTSDEEKHREFARVNRIQLA
GSEDTEQPLLNFKNEGDQSVREFKPSVSKNIHLLSQLSLNYFSNGFSCSEPHDTELFF
AVIYLAPGDYHHFHSPVDWVCKVRRHFPGDLFSVAPYFQRNFPNLFVLNERVALLGSW
KYGFFSMTPVGATNVGSIKLNFDQEFVTNSKSDKHLEPHTCYQAVYENASKILGGMPL
VKGEEMGGFELGSTVVLCFEAPTEFKFDVRVGDKVKMGQKLGIIGKNDLK"
gene complement(<318030..>318809)
/gene="FMP41"
/locus_tag="YNL168C"
/db_xref="GeneID:855553"
mRNA complement(<318030..>318809)
/gene="FMP41"
/locus_tag="YNL168C"
/product="Fmp41p"
/transcript_id="NM_001183006.1"
/db_xref="GeneID:855553"
CDS complement(318030..318809)
/gene="FMP41"
/locus_tag="YNL168C"
/EC_number="5.3.2.2"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:24769239|PMID:16823961|PMID:14562095|PMID:14576278]"
/note="hypothetical protein; GFP-fusion protein is induced
in response to the DNA-damaging agent MMS; the authentic,
non-tagged protein is detected in highly purified
mitochondria in high-throughput studies"
/codon_start=1
/product="Fmp41p"
/protein_id="NP_014231.1"
/db_xref="GeneID:855553"
/db_xref="SGD:S000005112"
/translation="MSYNYLKAARKIICIGRNYAAHIKELNNSTPKQPFFFLKPTSSI
VTPLSSSLVKTTRPANSTFNGLNEDGTNPGPIFIPRGVKVHHEIELALIVSKHLSNVT
KMKPEEVYDSISGVALALDLTARNVQDEAKKKGLPWTISKGFDTFMPISAIVSREKFS
SYKSNLQDIFRVKCSVNGQLRQDGGTNLMLHPLHKILQHISTMISLEPGDIILTGTPA
GVGELKPGDRVHCELLQNNDNIVDMNFECENRPGPYEFRET"
gene complement(<319416..>321359)
/gene="SKO1"
/locus_tag="YNL167C"
/gene_synonym="ACR1"
/db_xref="GeneID:855554"
mRNA complement(<319416..>321359)
/gene="SKO1"
/locus_tag="YNL167C"
/gene_synonym="ACR1"
/product="Sko1p"
/transcript_id="NM_001183005.1"
/db_xref="GeneID:855554"
CDS complement(319416..321359)
/gene="SKO1"
/locus_tag="YNL167C"
/gene_synonym="ACR1"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding
[PMID:11500510|PMID:11113177|PMID:9858577|PMID:1437546|PMI
D:12086627|PMID:1448073]"
/experiment="EXISTENCE:direct assay:GO:0001227 DNA-binding
transcription repressor activity, RNA polymerase
II-specific
[PMID:1437546|PMID:1448073|PMID:11113177|PMID:9858577]"
/experiment="EXISTENCE:direct assay:GO:0001228 DNA-binding
transcription activator activity, RNA polymerase
II-specific [PMID:12086627]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11500510]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:11500510]"
/experiment="EXISTENCE:direct assay:GO:0045944 positive
regulation of transcription by RNA polymerase II
[PMID:12086627]"
/experiment="EXISTENCE:direct assay:GO:0051019
mitogen-activated protein kinase binding [PMID:11230135]"
/experiment="EXISTENCE:direct assay:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:11230135|PMID:11500510]"
/experiment="EXISTENCE:direct assay:GO:0071470 cellular
response to osmotic stress [PMID:12086627]"
/experiment="EXISTENCE:genetic interaction:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:9858577]"
/experiment="EXISTENCE:genetic interaction:GO:0003714
transcription corepressor activity [PMID:11230135]"
/experiment="EXISTENCE:genetic interaction:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:11230135|PMID:11113177]"
/experiment="EXISTENCE:genetic interaction:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:11230135]"
/experiment="EXISTENCE:genetic interaction:GO:0071470
cellular response to osmotic stress
[PMID:11113177|PMID:11230135]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:11230135|PMID:1448073|PMID:9858577|PMID:11113177|PMI
D:10825197|PMID:1437546]"
/experiment="EXISTENCE:mutant phenotype:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:11113177]"
/experiment="EXISTENCE:mutant phenotype:GO:0000981
DNA-binding transcription factor activity, RNA polymerase
II-specific [PMID:12086627]"
/experiment="EXISTENCE:mutant phenotype:GO:0001227
DNA-binding transcription repressor activity, RNA
polymerase II-specific
[PMID:1437546|PMID:1448073|PMID:11113177|PMID:9858577]"
/experiment="EXISTENCE:mutant phenotype:GO:0001228
DNA-binding transcription activator activity, RNA
polymerase II-specific [PMID:12086627]"
/experiment="EXISTENCE:mutant phenotype:GO:0003714
transcription corepressor activity [PMID:11230135]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:12086627|PMID:10825197|PMID:11230135|PMID:11113177]"
/experiment="EXISTENCE:mutant phenotype:GO:0051019
mitogen-activated protein kinase binding [PMID:12086627]"
/experiment="EXISTENCE:mutant phenotype:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:11230135]"
/experiment="EXISTENCE:mutant phenotype:GO:0071470
cellular response to osmotic stress
[PMID:11230135|PMID:12086627|PMID:11113177|PMID:10825197]"
/note="Basic leucine zipper transcription factor of the
ATF/CREB family; forms a complex with Tup1p and Cyc8p to
both activate and repress transcription; cytosolic and
nuclear protein involved in osmotic and oxidative stress
responses"
/codon_start=1
/product="Sko1p"
/protein_id="NP_014232.1"
/db_xref="GeneID:855554"
/db_xref="SGD:S000005111"
/translation="MSSEERSRQPSTVSTFDLEPNPFEQSFASSKKALSLPGTISHPS
LPKELSRNNSTSTITQHSQRSTHSLNSIPEENGNSTVTDNSNHNDVKKDSPSFLPGQQ
RPTIISPPILTPGGSKRLPPLLLSPSILYQANSTTNPSQNSHSVSVSNSNPSAIGVSS
TSGSLYPNSSSPSGTSLIRQPRNSNVTTSNSGNGFPTNDSQMPGFLLNLSKSGLTPNE
SNIRTGLTPGILTQSYNYPVLPSINKNTITGSKNVNKSVTVNGSIENHPHVNIMHPTV
NGTPLTPGLSSLLNLPSTGVLANPVFKSTPTTNTTDGTVNNSISNSNFSPNTSTKAAV
KMDNPAEFNAIEHSAHNHKENENLTTQIENNDQFNNKTRKRKRRMSSTSSTSKASRKN
SISRKNSAVTTAPAQKDDVENNKISNNVTLDENEEQERKRKEFLERNRVAASKFRKRK
KEYIKKIENDLQFYESEYDDLTQVIGKLCGIIPSSSSNSQFNVNVSTPSSSSPPSTSL
IALLESSISRSDYSSAMSVLSNMKQLICETNFYRRGGKNPRDDMDGQEDSFNKDTNVV
KSENAGYPSVNSRPIILDKKYSLNSGANISKSNTTTNNVGNSAQNIINSCYSVTNPLV
INANSDTHDTNKHDVLSTLPHNN"
rep_origin 321921..322213
/note="ARS1415; Autonomously Replicating Sequence"
/db_xref="SGD:S000118385"
gene complement(<322219..>323565)
/gene="BNI5"
/locus_tag="YNL166C"
/db_xref="GeneID:855555"
mRNA complement(<322219..>323565)
/gene="BNI5"
/locus_tag="YNL166C"
/product="Bni5p"
/transcript_id="NM_001183004.1"
/db_xref="GeneID:855555"
CDS complement(322219..323565)
/gene="BNI5"
/locus_tag="YNL166C"
/experiment="EXISTENCE:direct assay:GO:0000144 cellular
bud neck septin ring [PMID:12215547]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:22842922|PMID:12215547]"
/experiment="EXISTENCE:genetic interaction:GO:0030674
protein-macromolecule adaptor activity [PMID:25971666]"
/experiment="EXISTENCE:mutant phenotype:GO:0000921 septin
ring assembly [PMID:12215547]"
/experiment="EXISTENCE:mutant phenotype:GO:0072741 protein
localization to cell division site [PMID:21173112]"
/experiment="EXISTENCE:physical interaction:GO:0032035
myosin II tail binding [PMID:21173112]"
/note="Linker protein responsible for recruitment of
myosin to the bud neck; interacts with the C-terminal
extensions of septins Cdc11p and Shs1p and binds Myo1p to
promote cytokinesis"
/codon_start=1
/product="Bni5p"
/protein_id="NP_014233.1"
/db_xref="GeneID:855555"
/db_xref="SGD:S000005110"
/translation="MGLDQDKIKKRLSQIEIDINQMNQMIDENLQLVEPAEDEAVEDN
VKDTGVVDAVKVAETALFSGNDGADSNPGDSAQVEEHKTAQVHIPTENEANKSTDDPS
QLSVTQPFIAKEQITHTAIAIGDSYNSFVANSAGNEKAKDSCTENKEDGTVNIDQNRG
EADVEIIENNDDEWEDEKSDVEEGRVDKGTEENSEIESFKSPMPQNNTLGGENKLDAE
LVLDKFSSANKDLDIQPQTIVVGGDNEYNHESSRLADQTPHDDNSENCPNRSGGSTPL
DSQTKIFIPKKNSKEDGTNINHFNSDGDGQKKMANFETRRPTNPFRVISVSSNSNSRN
GSRKSSLNKYDSPVSSPITSASELGSIAKLEKRHDYLSMKCIKLQKEIDYLNKMNAQG
SLSMEDGKRLHRAVVKLQEYLDKKTKEKYEVGVLLSRHLRKQIDRGENGQFWIGTK"
gene <323832..>325052
/locus_tag="YNL165W"
/db_xref="GeneID:855556"
mRNA <323832..>325052
/locus_tag="YNL165W"
/product="uncharacterized protein"
/transcript_id="NM_001183003.3"
/db_xref="GeneID:855556"
CDS 323832..325052
/locus_tag="YNL165W"
/note="hypothetical protein; YNL165W is not an essential
gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014234.3"
/db_xref="GeneID:855556"
/db_xref="SGD:S000005109"
/translation="MDRVRSLIGNRRGRRHNRQHPPYPHSGSPSTVNLLGANGYGDDQ
STIFARENESLETSANEGDDSADAATLNTAVSEGSTIGDLQRQGYVNRAPRFTSERAM
PFVSVLLQRGFFAFPSEESLQLFLHNKRKLDNIDPKRGLGLPLFHAISLNLVKSLFSD
QNTPVMRIYKYVMIDSQCDKPPLNSEVVSRINENVSIYKYEFCTILKKMESHNFSSRV
EHDFIFHRKDEPDVHIPMINYNQRKNADTAIHGLNLRWYGTTSLASPFGSNSINLLVL
DDTMASYMNQQTIEEFDSYSRSRPTRPLGYLPVWARYTDDKVSVIPKKRTLRVATLYL
QETDSFDDGSSLTSTNYTEMGSNIIENVPWDSQILTCMCMLLHEYESRKEKRHTAWGS
STTYMLNGPAGLLM"
rep_origin 325054..325267
/note="ARS1416; Putative replication origin; identified in
multiple array studies, not yet confirmed by plasmid-based
assay"
/db_xref="SGD:S000130142"
gene complement(<325265..>326320)
/gene="IBD2"
/locus_tag="YNL164C"
/db_xref="GeneID:855557"
mRNA complement(<325265..>326320)
/gene="IBD2"
/locus_tag="YNL164C"
/product="Ibd2p"
/transcript_id="NM_001183002.1"
/db_xref="GeneID:855557"
CDS complement(325265..326320)
/gene="IBD2"
/locus_tag="YNL164C"
/experiment="EXISTENCE:genetic interaction:GO:0007094
mitotic spindle assembly checkpoint signaling
[PMID:12072457]"
/experiment="EXISTENCE:mutant phenotype:GO:0007094 mitotic
spindle assembly checkpoint signaling [PMID:12072457]"
/note="Component of the BUB2-dependent spindle checkpoint
pathway; interacts with Bfa1p and functions upstream of
Bub2p and Bfa1p"
/codon_start=1
/product="Ibd2p"
/protein_id="NP_014235.1"
/db_xref="GeneID:855557"
/db_xref="SGD:S000005108"
/translation="MTPTNQSSGTTNASVEVLSEDGPMPINVMMQEGVKALTKILSNQ
LQDRQAFQNAPHAMQFVIRNGGKALSNARLEELKDALPKMDSLSLEDELAKIDGQSAY
HIDSAEEKETFESKIGQIASRNSADFIIEEDLQNILDDDLKDSELNLDGEEAEIIFDY
ESQELDTPDGIGEKISQMIESVLPGGFGSEEQGGLRTVTNVEDLDVAEEVTDIDHDTV
DAARLHGDGQHSISSRKHSRSKNSKKNGHVRRHDFYDESRDHKSCCPHHHYENLSKLR
NYYYHDFEYISRTENRVPDFSVLVNESSPMCLFCEYYMVFGEPPRNMIKWYNRTFGYN
RMPNPPRDEQDSRKRNR"
gene complement(<326741..>330073)
/gene="RIA1"
/locus_tag="YNL163C"
/gene_synonym="EFL1"
/db_xref="GeneID:855558"
mRNA complement(<326741..>330073)
/gene="RIA1"
/locus_tag="YNL163C"
/gene_synonym="EFL1"
/product="GTPase RIA1"
/transcript_id="NM_001183001.1"
/db_xref="GeneID:855558"
CDS complement(326741..330073)
/gene="RIA1"
/locus_tag="YNL163C"
/gene_synonym="EFL1"
/experiment="EXISTENCE:direct assay:GO:0003924 GTPase
activity [PMID:11779510]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11779510]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0042256
cytosolic ribosome assembly [PMID:11779510]"
/note="Cytoplasmic GTPase/eEF2-like factor involved in
ribosomal biogenesis; with Sdo1p, a guanine nucleotide
exchange factor (GEF), promotes release of Tif6p from 60S
ribosomal subunits in the cytoplasm so that they can
assemble with 40S subunits to generate mature ribosomes;
required for quality control check of newly made large
ribosomal subunits before they are released into the pool
of translating ribosomes"
/codon_start=1
/product="GTPase RIA1"
/protein_id="NP_014236.1"
/db_xref="GeneID:855558"
/db_xref="SGD:S000005107"
/translation="MPRVESETYKRLQNDPSCIRNICIVAHVDHGKTSLSDSLLASNG
IISQRLAGKIRFLDARPDEQLRGITMESSAISLYFRVLRKQEGSDEPLVSEHLVNLID
SPGHIDFSSEVSAASRLCDGAVVLVDVVEGVCSQTVTVLRQCWTEKLKPILVLNKIDR
LITELQLTPQEAYIHLSKVIEQVNSVIGSFFANERQLDDLFWREQLEKNENAEYIEKD
DSGIYFNPTDNNVIFASAIDGWGFNIGQLAKFYEQKLGAKRENLQKVLWGDFYMDPKT
KKIINNKGLKGRSLKPLFTSLILENIWKIYQNIITSRDSEMVEKIAKTLNIKLLARDL
RSKDDKQLLRTIMGQWLPVSTAVLLTVIEKLPSPLESQTDRLNTILVSESDTAAMDPR
LLKAMKTCDKEGPVSAYVSKMLSIPREELPVESKRIASSDELMERSRKAREEALNAAK
HAGMVENMAMMDLNDNSKNTSDLYKRAKDTVMTPEVGEQTKPKPSRNNDVFCVVSEPS
SALDLEFEYEGEDDSDSQDNFGLDFVPTDIDPNDPLSSMFEYEEEDPLLESIKQISED
VNDEVDDIFDEKEECLVAFARIYSGTLRVGQEISVLGPKYDPKCPEEHIETAIITHLY
LFMGKELVPLDVCPSGNIVGIRGLAGKVLKSGTLIEKGVQGVNLAGVNFHFTPIVRVA
VEPANPVEMSKLVRGLKLLDQADPCVHTYVENTGEHILCTAGELHLERCLKDLTERFA
GIEITHSEPAIPYRETFLSASDMNPPQNSQLGRGVHELLLSQYKITFRTFPLSGKVTD
FLSQHQNSIKNILKTSTSSMDPVIESTGSSFLDKKSLLVAFEEVINQEEKSRELLSGF
KVKLAGFGPSRVGCNILLSQDNLLGSLFEGTPAAFEYSDSIKNGFQLAVSEGPLANEP
VQGMCVLVESVHKMSQDEIESIEDPRYQQHIVDLSGRLITSTRDAIHEAFLDWSPRIM
WAIYSCDIQTSVDVLGKVYAVILQRHGKIISEEMKEGTPFFQIEAHVPVVEAFGLSED
IRKRTSGAAQPQLVFSGFECIDLDPFWVPTTEEELEELGDTADRENIARKHMNAIRRR
KGLFIEEKVVENAEKQRTLKKN"
gene <330329..>330547
/locus_tag="YNL162W-A"
/db_xref="GeneID:855559"
mRNA <330329..>330547
/locus_tag="YNL162W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184490.1"
/db_xref="GeneID:855559"
CDS 330329..330547
/locus_tag="YNL162W-A"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="hypothetical protein; identified by homology"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_076906.1"
/db_xref="GeneID:855559"
/db_xref="SGD:S000007624"
/translation="MSDKPDSQVFCPNCNERLQKCLVQQNYAIIICPSLVCGYPFNQR
EVLENLTYVDDNDVLKVAKKRLSSRSKP"
gene <331322..>332154
/gene="RPL42A"
/locus_tag="YNL162W"
/db_xref="GeneID:855560"
mRNA join(<331322..331325,331838..>332154)
/gene="RPL42A"
/locus_tag="YNL162W"
/product="60S ribosomal protein eL42 RPL42A"
/transcript_id="NM_001183000.1"
/db_xref="GeneID:855560"
CDS join(331322..331325,331838..332154)
/gene="RPL42A"
/locus_tag="YNL162W"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:11983894]"
/experiment="EXISTENCE:curator inference:GO:0003735
structural constituent of ribosome [PMID:11983894]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:11983894]"
/note="Ribosomal 60S subunit protein L42A; homologous to
mammalian ribosomal protein L36A, no bacterial homolog;
RPL42A has a paralog, RPL42B, that arose from the whole
genome duplication"
/codon_start=1
/product="60S ribosomal protein eL42 RPL42A"
/protein_id="NP_014237.2"
/db_xref="GeneID:855560"
/db_xref="SGD:S000005106"
/translation="MVNVPKTRKTYCKGKTCRKHTQHKVTQYKAGKASLFAQGKRRYD
RKQSGFGGQTKPVFHKKAKTTKKVVLRLECVKCKTRAQLTLKRCKHFELGGEKKQKGQ
ALQF"
gene <332597..>334867
/gene="CBK1"
/locus_tag="YNL161W"
/db_xref="GeneID:855561"
mRNA <332597..>334867
/gene="CBK1"
/locus_tag="YNL161W"
/product="serine/threonine protein kinase CBK1"
/transcript_id="NM_001182999.3"
/db_xref="GeneID:855561"
CDS 332597..334867
/gene="CBK1"
/locus_tag="YNL161W"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:10970846]"
/experiment="EXISTENCE:direct assay:GO:0004674 protein
serine/threonine kinase activity [PMID:18715118]"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12196508|PMID:11747810]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:10970846|PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005933 cellular
bud [PMID:12196508]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:22842922|PMID:11747810|PMID:10970846]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck
[PMID:10970846|PMID:22842922|PMID:11259593|PMID:12196508|P
MID:11747810]"
/experiment="EXISTENCE:direct assay:GO:0005938 cell cortex
[PMID:10970846]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807|PMID:11747810]"
/experiment="EXISTENCE:genetic interaction:GO:0007118
budding cell apical bud growth
[PMID:12972564|PMID:11259593]"
/experiment="EXISTENCE:genetic interaction:GO:0060237
regulation of fungal-type cell wall organization
[PMID:19962308]"
/experiment="EXISTENCE:mutant phenotype:GO:0000920 septum
digestion after cytokinesis [PMID:12196508|PMID:11747810]"
/experiment="EXISTENCE:mutant phenotype:GO:0007118 budding
cell apical bud growth [PMID:12972564|PMID:11259593]"
/experiment="EXISTENCE:mutant phenotype:GO:0007163
establishment or maintenance of cell polarity
[PMID:12196508]"
/experiment="EXISTENCE:mutant phenotype:GO:0030950
establishment or maintenance of actin cytoskeleton
polarity [PMID:11259593]"
/experiment="EXISTENCE:mutant phenotype:GO:0050708
regulation of protein secretion [PMID:30455324]"
/note="Serine/threonine protein kinase of the the RAM
signaling network; Ndr/LATS family member; binds
regulatory subunit Mob2p; involved in regulation of
cellular morphogenesis, polarized growth, and septum
destruction; phosphorylation by Cbk1p regulates
localization and activity of Ace2p transcription factor
and Ssd1p translational repressor; Cbk1p activity is
regulated by both phosphorylation and specific
localization; relocalizes to cytoplasm upon DNA
replication stress"
/codon_start=1
/product="serine/threonine protein kinase CBK1"
/protein_id="NP_014238.3"
/db_xref="GeneID:855561"
/db_xref="SGD:S000005105"
/translation="MYNSSTNHHEGAPTSGHGYYMSQQQDQQHQQQQQYANEMNPYQQ
IPRPPAAGFSSNYMKEQGSHQSLQEHLQRETGNLGSGFTDVPALNYPATPPPHNNYAA
SNQMINTPPPSMGGLYRHNNNSQSMVQNGNGSGNAQLPQLSPGQYSIESEYNQNLNGS
SSSSPFHQPQTLRSNGSYSSGLRSVKSFQRLQQEQENVQVQQQLSQAQQQNSRQQQQQ
LQYQQQQQQQQQQQHMQIQQQQQQQQQQQQSQSPVQSGFNNGTISNYMYFERRPDLLT
KGTQDKAAAVKLKIENFYQSSVKYAIERNERRVELETELTSHNWSEERKSRQLSSLGK
KESQFLRLRRTRLSLEDFHTVKVIGKGAFGEVRLVQKKDTGKIYAMKTLLKSEMYKKD
QLAHVKAERDVLAGSDSPWVVSLYYSFQDAQYLYLIMEFLPGGDLMTMLIRWQLFTED
VTRFYMAECILAIETIHKLGFIHRDIKPDNILIDIRGHIKLSDFGLSTGFHKTHDSNY
YKKLLQQDEATNGISKPGTYNANTTDTANKRQTMVVDSISLTMSNRQQIQTWRKSRRL
MAYSTVGTPDYIAPEIFLYQGYGQECDWWSLGAIMYECLIGWPPFCSETPQETYRKIM
NFEQTLQFPDDIHISYEAEDLIRRLLTHADQRLGRHGGADEIKSHPFFRGVDWNTIRQ
VEAPYIPKLSSITDTRFFPTDELENVPDSPAMAQAAKQREQMTKQGGSAPVKEDLPFI
GYTYSRFDYLTRKNAL"
gene <336545..>337609
/gene="YGP1"
/locus_tag="YNL160W"
/db_xref="GeneID:855562"
mRNA <336545..>337609
/gene="YGP1"
/locus_tag="YNL160W"
/product="Ygp1p"
/transcript_id="NM_001182998.1"
/db_xref="GeneID:855562"
CDS 336545..337609
/gene="YGP1"
/locus_tag="YNL160W"
/experiment="EXISTENCE:direct assay:GO:0005576
extracellular region [PMID:19129178]"
/experiment="EXISTENCE:direct assay:GO:0070726 cell wall
assembly [PMID:10234784]"
/note="Cell wall-related secretory glycoprotein; induced
by nutrient deprivation-associated growth arrest and upon
entry into stationary phase; may be involved in adaptation
prior to stationary phase entry; protein demonstrates
amyloid properties in vivo; YGP1 has a paralog, SPS100,
that arose from the whole genome duplication"
/codon_start=1
/product="Ygp1p"
/protein_id="NP_014239.1"
/db_xref="GeneID:855562"
/db_xref="SGD:S000005104"
/translation="MKFQVVLSALLACSSAVVASPIENLFKYRAVKASHSKNINSTLP
AWNGSNSSNVTYANGTNSTTNTTTAESSQLQIIVTGGQVPITNSSLTHTNYTRLFNSS
SALNITELYNVARVVNETIQDKSSAGAVVVANAKSLEAVSFFFSIIFDTEKPIVVTED
SAYAIPVANNKNATKRGVLSVTSDKLVYSGVFTPPTACSYGAGLPVAIVDDQDEVKWF
FDASKPTLISSDSIIRKEYSNFTTPYGLLENGVPIVPIVYDGGYSSSLIDSLSSAVQG
LVVVSSGSTNSTSSTIESTEIPVVYAQANTPLNFIDNKDVPKNAVGAGYLSPIKAQIL
LSIAAVNGVTSKSALESIFP"
gene complement(<338478..>339347)
/gene="ASI2"
/locus_tag="YNL159C"
/db_xref="GeneID:855563"
mRNA complement(<338478..>339347)
/gene="ASI2"
/locus_tag="YNL159C"
/product="Asi2p"
/transcript_id="NM_001182997.1"
/db_xref="GeneID:855563"
CDS complement(338478..339347)
/gene="ASI2"
/locus_tag="YNL159C"
/experiment="EXISTENCE:direct assay:GO:0005637 nuclear
inner membrane [PMID:17085444]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0097658 Asi complex
[PMID:25236469]"
/experiment="EXISTENCE:genetic interaction:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:11454748]"
/experiment="EXISTENCE:mutant phenotype:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:25236469]"
/experiment="EXISTENCE:mutant phenotype:GO:0043161
proteasome-mediated ubiquitin-dependent protein catabolic
process [PMID:25253722]"
/experiment="EXISTENCE:mutant phenotype:GO:0071230
cellular response to amino acid stimulus [PMID:17085444]"
/note="Subunit of the inner nuclear membrane Asi ubiquitin
ligase complex; the Asi complex targets both misfolded
proteins of the inner nuclear membrane-associated
degradation (INMAD) pathway and inner for
ubiquitin-mediated degradation; acts with Asi1p and Asi3p
to ensure the fidelity of SPS-sensor signaling by
targeting latent unprocessed forms of Stp1p and Stp2p,
maintaining the repressed state of gene expression in the
absence of inducing amino acids"
/codon_start=1
/product="Asi2p"
/protein_id="NP_014240.1"
/db_xref="GeneID:855563"
/db_xref="SGD:S000005103"
/translation="MARPQNHRRSNWTERDDNDDYLFQRFLEESETRHSREPSPVTEQ
SQQELQQDVQQAIDGIFNSLRRNMSSTSNINRAANMDATTNGNGGINADTIRATNANT
ADSPFTARQQSPLRTFLRNLFILDYFIGLILFPFSVYNILRSGFNSMTFSENDFIIEI
VGYWKFAKIFGSGGTTLIAYKDTGKLGLLGKFHNIIVFYSSPVIKHIMKSRDGNEPNL
NWIRLMFAKAFELFVKVSTILIYLAYGVSGTVYMVTAGFFFVLCLLFTVIRRYKGVHR
MLVSQRITGPGVF"
gene <339612..>340208
/gene="PGA1"
/locus_tag="YNL158W"
/db_xref="GeneID:855564"
mRNA <339612..>340208
/gene="PGA1"
/locus_tag="YNL158W"
/product="Pga1p"
/transcript_id="NM_001182996.1"
/db_xref="GeneID:855564"
CDS 339612..340208
/gene="PGA1"
/locus_tag="YNL158W"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:14690591]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:17615295]"
/experiment="EXISTENCE:genetic interaction:GO:0000030
mannosyltransferase activity [PMID:17615295]"
/experiment="EXISTENCE:genetic interaction:GO:0031501
mannosyltransferase complex [PMID:17615295]"
/experiment="EXISTENCE:mutant phenotype:GO:0000030
mannosyltransferase activity [PMID:17615295]"
/experiment="EXISTENCE:mutant phenotype:GO:0006506 GPI
anchor biosynthetic process [PMID:17615295]"
/experiment="EXISTENCE:physical interaction:GO:0031501
mannosyltransferase complex [PMID:17615295]"
/note="Essential component of GPI-mannosyltransferase II;
complex is responsible for second mannose addition to GPI
precursors as a partner of Gpi18p; required for maturation
of Gas1p and Pho8p; has synthetic genetic interactions
with secretory pathway genes"
/codon_start=1
/product="Pga1p"
/protein_id="NP_014241.1"
/db_xref="GeneID:855564"
/db_xref="SGD:S000005102"
/translation="MVRPQNVHWFIATIVFFIGFVHANTESILYKVPHNFPLKKPRDS
STYARDVNLISSISLSGEAMSQITIEANTTDLELHNTTYIELADLQRDETYQIKVCWS
AIHPISINNLQTITIPRFTEFQGTKSDYARILVTFQVLSDSYPSEHAMVPIQVSLITT
RLGIPVDIYPTLIVMVLLVAGLVVTRAPHVLNDLLLKF"
gene <340352..>340858
/gene="IGO1"
/locus_tag="YNL157W"
/db_xref="GeneID:855565"
mRNA <340352..>340858
/gene="IGO1"
/locus_tag="YNL157W"
/product="phosphatase regulator"
/transcript_id="NM_001182995.1"
/db_xref="GeneID:855565"
CDS 340352..340858
/gene="IGO1"
/locus_tag="YNL157W"
/experiment="EXISTENCE:direct assay:GO:0000932 P-body
[PMID:20471941]"
/experiment="EXISTENCE:direct assay:GO:0004865 protein
serine/threonine phosphatase inhibitor activity
[PMID:23273919|PMID:23861665]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:20471941|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0034605
cellular response to heat [PMID:23861665]"
/experiment="EXISTENCE:genetic interaction:GO:0048255 mRNA
stabilization [PMID:20471941]"
/experiment="EXISTENCE:genetic interaction:GO:1900152
negative regulation of nuclear-transcribed mRNA catabolic
process, deadenylation-dependent decay [PMID:20471941]"
/experiment="EXISTENCE:genetic interaction:GO:1901992
positive regulation of mitotic cell cycle phase transition
[PMID:23861665]"
/experiment="EXISTENCE:genetic interaction:GO:1903452
positive regulation of G1 to G0 transition
[PMID:23273919|PMID:20471941]"
/experiment="EXISTENCE:mutant phenotype:GO:0004865 protein
serine/threonine phosphatase inhibitor activity
[PMID:23273919]"
/note="Protein required for initiation of the G0 program;
prevents degradation of nutrient-regulated mRNAs via the
5'-3' mRNA decay pathway; phosphorylation (S63) by Rim15p
increases interaction with and inhibition of PP2A-Cdc55
for timely mitotic progression; can also activate
PP2A-Cdc55 complexes and influence Cdc55p localization
along with Igo2p; GFP protein localizes to the cytoplasm
and nucleus; IGO1 has a paralog, IGO2, that arose from the
whole genome duplication"
/codon_start=1
/product="phosphatase regulator"
/protein_id="NP_014242.1"
/db_xref="GeneID:855565"
/db_xref="SGD:S000005101"
/translation="MSNENLSPNSSNPDLTKLNNGESGTIDTSKFSPNEMKLYKMYGK
LPSKKDIFKHTMQKRKYFDSGDYALQKAGIQNNDPINYGKNNLPLTNPSKLREDIIKR
RISTCPSTASTAGVVDNATLIQKEGSISSGPPSSNNGTIGGGSTSSTPVGNHSSSSSS
LYTESPIR"
gene complement(<341069..>341968)
/gene="NSG2"
/locus_tag="YNL156C"
/db_xref="GeneID:855566"
mRNA complement(<341069..>341968)
/gene="NSG2"
/locus_tag="YNL156C"
/product="Nsg2p"
/transcript_id="NM_001182994.3"
/db_xref="GeneID:855566"
CDS complement(341069..341968)
/gene="NSG2"
/locus_tag="YNL156C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0071561
nucleus-vacuole junction [PMID:41132095]"
/experiment="EXISTENCE:genetic interaction:GO:0016126
sterol biosynthetic process [PMID:16270032]"
/experiment="EXISTENCE:mutant phenotype:GO:0051082
unfolded protein binding [PMID:16270032]"
/note="Protein involved in regulation of sterol
biosynthesis; stabilizes Hmg2p, one of two HMG-CoA
isoenzymes that catalyze the rate-limiting step in sterol
biosynthesis; localizes to the ER; localizes to the
nucleus-vacuole junction during glucose restriction;
homolog of mammalian INSIG proteins; NSG2 has a paralog,
NSG1, that arose from the whole genome duplication"
/codon_start=1
/product="Nsg2p"
/protein_id="NP_014243.3"
/db_xref="GeneID:855566"
/db_xref="SGD:S000005100"
/translation="MANRGEPDPKKSTESICSLTKPQLYSLYDDDVVRSEDNEIYEEL
KRSVSIDSTKYSRDQTIDSTFYLAHKVGGSLPRNTVSSNNLERILSASSIHENFPSRT
RQTRQNILHYLQAVLILSLSGFAYHELSRNLHDNHLLHPDFASRPLLLGVKLCNWLSN
GVLPNWLGYGVEGLLFGSVVPILDNIFQTEVVKSSVHHDSLTSVIRSINAMLGVTFGI
RKIQWNSSLQAAGAWGLLNIILWLFFDGSISMLMSCICIGVGCCISCYKDIIDGSQFL
YFMDFYFLGSLMFGKLGRYLYSH"
gene complement(<341911..>342135)
/locus_tag="YNL155C-A"
/db_xref="GeneID:91000626"
mRNA complement(<341911..>342135)
/locus_tag="YNL155C-A"
/product="uncharacterized protein"
/transcript_id="NM_001431142.1"
/db_xref="GeneID:91000626"
CDS complement(341911..342135)
/locus_tag="YNL155C-A"
/note="hypothetical protein; well conserved across
Saccharomyces species; added to genome annotation based on
proteomic, translation, and evolutionary evidence"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001418071.1"
/db_xref="GeneID:91000626"
/db_xref="SGD:S000350099"
/translation="MIGLIVVPILFAIKGIVVGVQKVKKKFGKHSNTKDQKEDKDEDK
RQSISQRKQHTEWPIEENRIQRRAPNQSAL"
gene <342516..>343340
/gene="CUZ1"
/locus_tag="YNL155W"
/db_xref="GeneID:855567"
mRNA <342516..>343340
/gene="CUZ1"
/locus_tag="YNL155W"
/product="Cuz1p"
/transcript_id="NM_001182993.1"
/db_xref="GeneID:855567"
CDS 342516..343340
/gene="CUZ1"
/locus_tag="YNL155W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0070628 proteasome
binding [PMID:24121501|PMID:24297164]"
/experiment="EXISTENCE:genetic interaction:GO:0043161
proteasome-mediated ubiquitin-dependent protein catabolic
process [PMID:24121501]"
/experiment="EXISTENCE:genetic interaction:GO:0071243
cellular response to arsenic-containing substance
[PMID:24297164]"
/experiment="EXISTENCE:mutant phenotype:GO:0043161
proteasome-mediated ubiquitin-dependent protein catabolic
process [PMID:24121501]"
/experiment="EXISTENCE:mutant phenotype:GO:0071243
cellular response to arsenic-containing substance
[PMID:24297164]"
/experiment="EXISTENCE:physical interaction:GO:0043161
proteasome-mediated ubiquitin-dependent protein catabolic
process [PMID:24121501]"
/note="Protein involved in Ub-proteasome dependent
catabolism; interacts with Cdc48p and the proteasome,
protecting cells from arsenite-induced proteotoxicity
through stress granule (SG) clearance; contains a PACE
promoter element; co-regulated with proteasome subunit
genes; contains an AN1-type zinc finger, a DHHC domain and
a UBL fold; complements SG clearance in arsenite treated
HeLa cells depleted of ZFAND1, a human ortholog linked to
cancer; protein abundance increases under DNA replication
stress"
/codon_start=1
/product="Cuz1p"
/protein_id="NP_014244.1"
/db_xref="GeneID:855567"
/db_xref="SGD:S000005099"
/translation="MSAAIEKETGMLDVGKHCAYCRQLDFLPFHCSFCNEDFCSNHRL
KEDHHCRWLLEHEEVHKTEKSPSKSRDGSSSNDEAYFKSLLPERASVRIQRVSETREP
LRGSNTAKVSSTLNSKTLDKIFKFFQRNEKRKSNNKSKKNFGSSSNKIIQLANLKKIA
KGDPKIPMQNRIYIWCYLVDGDETDIAKEDTRMPLYINKMWPVGRAMDYLSIQLNVKS
STLTNSSSNDKFQLCKLKEGKQVSFYNIGASLRVTNEIKDLDTLYLVHNNADEKSN"
gene complement(<343636..>345276)
/gene="YCK2"
/locus_tag="YNL154C"
/db_xref="GeneID:855568"
mRNA complement(<343636..>345276)
/gene="YCK2"
/locus_tag="YNL154C"
/product="serine/threonine protein kinase YCK2"
/transcript_id="NM_001182992.1"
/db_xref="GeneID:855568"
CDS complement(343636..345276)
/gene="YCK2"
/locus_tag="YNL154C"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0004674 protein
serine/threonine kinase activity [PMID:8474447]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane
[PMID:1627830|PMID:16622836|PMID:12469340|PMID:8034689]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:10198058]"
/experiment="EXISTENCE:direct assay:GO:0005937 mating
projection [PMID:10198058]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0000902 cell
morphogenesis [PMID:8474447]"
/experiment="EXISTENCE:genetic interaction:GO:0010255
glucose mediated signaling pathway [PMID:27630263]"
/experiment="EXISTENCE:mutant phenotype:GO:0006897
endocytosis [PMID:10198058]"
/experiment="EXISTENCE:mutant phenotype:GO:0009749
response to glucose [PMID:14755054]"
/note="Palmitoylated plasma membrane-bound casein kinase I
(CK1) isoform; shares redundant functions with Yck1p in
morphogenesis, proper septin assembly, endocytic
trafficking, and glucose sensing; stabilized by Sod1p
binding in the presence of glucose and oxygen, causing
glucose repression of respiratory metabolism; involved in
the phosphorylation and regulation of glucose sensor
Rgt2p; YCK2 has a paralog, YCK1, that arose from the whole
genome duplication"
/codon_start=1
/product="serine/threonine protein kinase YCK2"
/protein_id="NP_014245.1"
/db_xref="GeneID:855568"
/db_xref="SGD:S000005098"
/translation="MSQVQSPLTATNSGLAVNNNTMNSQMPNRSNVRLVNGTLPPSLH
VSSNLNHNTGNSSASYSGSQSRDDSTIVGLHYKIGKKIGEGSFGVLFEGTNMINGLPV
AIKFEPRKTEAPQLKDEYRTYKILAGTPGIPQEYYFGQEGLHNILVIDLLGPSLEDLF
DWCGRRFSVKTVVQVAVQMITLIEDLHAHDLIYRDIKPDNFLIGRPGQPDANKVHLID
FGMAKQYRDPKTKQHIPYREKKSLSGTARYMSINTHLGREQSRRDDMEAMGHVFFYFL
RGQLPWQGLKAPNNKQKYEKIGEKKRLTNVYDLAQGLPIQFGRYLEIVRNLSFEETPD
YEGYRMLLLSVLDDLGETADGQYDWMKLNGGRGWDLSINKKPNLHGYGHPNPPNEKSK
RHRSKNHQYSSPDHHHHYNQQQQQQQAQAQAQAQAQAKVQQQQLQQAQAQQQANRYQL
QPDDSHYDEEREASKLDPTSYEAYQQQTQQKYAQQQQKQMQQKSKQFANTGANGQTNK
YPYNAQPTANDEQNAKNAAQDRNSNKSSKGFFSKLGCC"
gene complement(<345669..>346058)
/gene="GIM3"
/locus_tag="YNL153C"
/gene_synonym="PFD4"
/db_xref="GeneID:855569"
mRNA complement(<345669..>346058)
/gene="GIM3"
/locus_tag="YNL153C"
/gene_synonym="PFD4"
/product="tubulin-binding prefolding complex subunit GIM3"
/transcript_id="NM_001182991.1"
/db_xref="GeneID:855569"
CDS complement(345669..346058)
/gene="GIM3"
/locus_tag="YNL153C"
/gene_synonym="PFD4"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:9463374]"
/experiment="EXISTENCE:direct assay:GO:0015631 tubulin
binding [PMID:9463374]"
/experiment="EXISTENCE:genetic interaction:GO:0032968
positive regulation of transcription elongation by RNA
polymerase II [PMID:24068951]"
/experiment="EXISTENCE:mutant phenotype:GO:0007021 tubulin
complex assembly [PMID:9463374]"
/experiment="EXISTENCE:mutant phenotype:GO:0032968
positive regulation of transcription elongation by RNA
polymerase II [PMID:24068951]"
/experiment="EXISTENCE:mutant phenotype:GO:0071629
cytoplasm protein quality control by the
ubiquitin-proteasome system [PMID:27448207]"
/experiment="EXISTENCE:physical interaction:GO:0016272
prefoldin complex [PMID:9463374]"
/note="Subunit of the heterohexameric cochaperone
prefoldin complex; prefoldin binds specifically to
cytosolic chaperonin and transfers target proteins to it;
prefoldin complex also localizes to chromatin of actively
transcribed genes in the nucleus and facilitates
transcriptional elongation"
/codon_start=1
/product="tubulin-binding prefolding complex subunit GIM3"
/protein_id="NP_014246.1"
/db_xref="GeneID:855569"
/db_xref="SGD:S000005097"
/translation="MELLPQGQRNNTQVTFEDQQKINEFSKLIMRKDAIAQELSLQRE
EKEYLDDVSLEIELIDEDEPVQYKVGDLFIFMKQSKVTAQLEKDAERLDNKIETLEDK
QRDIDSRLDALKAILYAKFGDNINLER"
gene <346313..>347542
/gene="INN1"
/locus_tag="YNL152W"
/db_xref="GeneID:855570"
mRNA <346313..>347542
/gene="INN1"
/locus_tag="YNL152W"
/product="Inn1p"
/transcript_id="NM_001182990.1"
/db_xref="GeneID:855570"
CDS 346313..347542
/gene="INN1"
/locus_tag="YNL152W"
/experiment="EXISTENCE:direct assay:GO:0000142 cellular
bud neck contractile ring [PMID:18344988]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:11914276]"
/experiment="EXISTENCE:genetic interaction:GO:0030234
enzyme regulator activity [PMID:22956544]"
/experiment="EXISTENCE:genetic interaction:GO:1990344
secondary cell septum biogenesis [PMID:19528296]"
/experiment="EXISTENCE:mutant phenotype:GO:0000281 mitotic
cytokinesis [PMID:22956544|PMID:18344988]"
/experiment="EXISTENCE:mutant phenotype:GO:0051276
chromosome organization [PMID:18439903]"
/experiment="EXISTENCE:mutant phenotype:GO:1990344
secondary cell septum biogenesis [PMID:19528296]"
/experiment="EXISTENCE:physical interaction:GO:0044697
HICS complex [PMID:22623719]"
/note="Essential protein that associates with contractile
actomyosin ring; required for ingression of the plasma
membrane into the bud neck during cytokinesis; C2 domain,
a membrane targeting module, is required for function;
activates chitin synthase activity of Chs2p during
cytokinesis"
/codon_start=1
/product="Inn1p"
/protein_id="NP_014247.1"
/db_xref="GeneID:855570"
/db_xref="SGD:S000005096"
/translation="MSEEVWNGNQGILSVYVSKARDLPNLNKLDKQNVMLRLRIAHMT
RASNTLHRAGQNPVFHYLEKFDITPEIKPLMYVEVYCDRRKKSPLPIGRCEIDLLNAI
RADPKEGYCTWYELKRSGDEFAGTIFIELTFTPKVPRLNRDDLNKEMDRLDSSMAMRP
IPPLPTESEYDYVHGSTMRQITPQCVSTSHEDKDEGQPYRNGNVFSMSSKSDTAVLAN
SNDPIILPPTFSASMGTTSTLETNDTAISNTSNTKFHFANLRKLKEKINIFKNPDSST
NNCQNESNKVDIEALQKAIGVTSLSYDEDDDDDDENDAFYSSSHRVSHNYNQPPLPPI
PTRDDMSNYSSSRNTPLVRRDRPSRLDSSSPNSHPHPSGLNSPKLPPLPTTSNSNFNS
RKNSMSPTRKRPPPRLS"
gene complement(<347766..>348521)
/gene="RPC31"
/locus_tag="YNL151C"
/gene_synonym="ACP2; RPC8"
/db_xref="GeneID:855571"
mRNA complement(<347766..>348521)
/gene="RPC31"
/locus_tag="YNL151C"
/gene_synonym="ACP2; RPC8"
/product="DNA-directed RNA polymerase III subunit C31"
/transcript_id="NM_001182989.3"
/db_xref="GeneID:855571"
CDS complement(347766..348521)
/gene="RPC31"
/locus_tag="YNL151C"
/gene_synonym="ACP2; RPC8"
/experiment="EXISTENCE:direct assay:GO:0003899
DNA-directed RNA polymerase activity [PMID:3905793]"
/experiment="EXISTENCE:direct assay:GO:0005666 RNA
polymerase III complex [PMID:3905793|PMID:10611227]"
/experiment="EXISTENCE:direct assay:GO:0042797 tRNA
transcription by RNA polymerase III [PMID:3905793]"
/experiment="EXISTENCE:mutant phenotype:GO:0006384
transcription initiation at RNA polymerase III promoter
[PMID:7835345]"
/experiment="EXISTENCE:mutant phenotype:GO:0042797 tRNA
transcription by RNA polymerase III [PMID:2201900]"
/note="RNA polymerase III subunit C31"
/codon_start=1
/product="DNA-directed RNA polymerase III subunit C31"
/protein_id="NP_014248.3"
/db_xref="GeneID:855571"
/db_xref="SGD:S000005095"
/translation="MSSYRGGSRGGGSNYMSNLPFGLGYGDVGKNHITEFPSIPLPIN
GPITNKERSLAVKYINFGKTVKDGPFYTGSMSLIIDQQENSKSGKRKPNIILDEDDTN
DGIERYSDKYLKKRKIGISIDDHPYNLNLFPNELYNVMGINKKKLLAISKFNNADDVF
TGTGLQDENIGLSMLAKLKELAEDVDDASTGDGAAKGSKTGEGEDDDLADDDFEEDED
EEDDDDYNAEKYFNNGDDDDYGDEEDPNEEAAF"
gene complement(<349367..>349756)
/gene="PGA2"
/locus_tag="YNL149C"
/db_xref="GeneID:855573"
mRNA complement(<349367..>349756)
/gene="PGA2"
/locus_tag="YNL149C"
/product="Pga2p"
/transcript_id="NM_001182987.1"
/db_xref="GeneID:855573"
CDS complement(349367..349756)
/gene="PGA2"
/locus_tag="YNL149C"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:14690591]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095|PMID:14690591|PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0015031 protein
transport [PMID:16943325]"
/note="Essential protein required for maturation of Gas1p
and Pho8p; involved in protein trafficking; GFP-fusion
protein localizes to the ER and YFP-fusion protein to the
nuclear envelope-ER network; null mutants have a cell
separation defect"
/codon_start=1
/product="Pga2p"
/protein_id="NP_014250.1"
/db_xref="GeneID:855573"
/db_xref="SGD:S000005093"
/translation="MSEVAETWVDTWMAKLVNYDYKHFIRLVIIVGGYLLLRNIASRE
LAKKQLAAQVEKDKRDKEEKRSKDLIDKPDDAATAETTSFGWGKKTRRRVKRQQELFE
NALEEAKRRNQGLDPDSDADIEELLEE"
gene complement(<349907..>350671)
/gene="ALF1"
/locus_tag="YNL148C"
/db_xref="GeneID:855574"
mRNA complement(<349907..>350671)
/gene="ALF1"
/locus_tag="YNL148C"
/product="Alf1p"
/transcript_id="NM_001182986.1"
/db_xref="GeneID:855574"
CDS complement(349907..350671)
/gene="ALF1"
/locus_tag="YNL148C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0007023
post-chaperonin tubulin folding pathway [PMID:9885248]"
/experiment="EXISTENCE:direct assay:GO:0008017 microtubule
binding [PMID:9885248]"
/experiment="EXISTENCE:mutant phenotype:GO:0006457 protein
folding [PMID:9885248]"
/experiment="EXISTENCE:mutant phenotype:GO:0007023
post-chaperonin tubulin folding pathway [PMID:9885248]"
/experiment="EXISTENCE:physical interaction:GO:0043014
alpha-tubulin binding [PMID:9885248]"
/note="Alpha-tubulin folding protein; similar to mammalian
cofactor B; Alf1p-GFP localizes to cytoplasmic
microtubules; required for the folding of alpha-tubulin
and may play an additional role in microtubule
maintenance"
/codon_start=1
/product="Alf1p"
/protein_id="NP_014251.1"
/db_xref="GeneID:855574"
/db_xref="SGD:S000005092"
/translation="MVRVVIESELVRTEKELPNSLKLRQFKDRLYHVTGVEPEDMEIV
VKRQYDNKEIYSTKKGGAYSNEDEDANFLKGEEELIVVVTDSNAQSISNQLATQAEGI
PSMEVISEEDYLRRDQSVLRWKMAHGYGRFNAAQQSQRAALAKQDEAYAREQLTAAIG
RHCRVTVDGSAPREAILRYVGPLPLDVMGTWCGVEFPEAAGKNDGRINGVTLFGPVAP
GHGSFVRPRAVEILSKDEESAEVEDVHDDVESDDEI"
gene <350940..>351383
/gene="LSM7"
/locus_tag="YNL147W"
/db_xref="GeneID:855575"
mRNA join(<350940..350957,351054..>351383)
/gene="LSM7"
/locus_tag="YNL147W"
/product="Sm-like protein LSM7"
/transcript_id="NM_001182985.1"
/db_xref="GeneID:855575"
CDS join(350940..350957,351054..351383)
/gene="LSM7"
/locus_tag="YNL147W"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:10369684]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005688 U6 snRNP
[PMID:10747033|PMID:10369684|PMID:11720284]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:18029398|PMID:15075370]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922|PMID:18029398]"
/experiment="EXISTENCE:direct assay:GO:0046540 U4/U6 x U5
tri-snRNP complex [PMID:10449419]"
/experiment="EXISTENCE:direct assay:GO:1990726 Lsm1-7-Pat1
complex [PMID:24139796]"
/experiment="EXISTENCE:mutant phenotype:GO:0000398 mRNA
splicing, via spliceosome [PMID:10428970|PMID:11333229]"
/experiment="EXISTENCE:mutant phenotype:GO:0000956
nuclear-transcribed mRNA catabolic process
[PMID:10747033]"
/experiment="EXISTENCE:mutant phenotype:GO:0030490
maturation of SSU-rRNA [PMID:19806183]"
/experiment="EXISTENCE:physical interaction:GO:0005732
sno(s)RNA-containing ribonucleoprotein complex
[PMID:15075370]"
/note="Lsm (Like Sm) protein; part of heteroheptameric
complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic
Lsm1p complex involved in mRNA decay; nuclear Lsm8p
complex part of U6 snRNP and possibly involved in
processing tRNA, snoRNA, and rRNA; protein abundance
increases and forms cytoplasmic foci in response to DNA
replication stress"
/codon_start=1
/product="Sm-like protein LSM7"
/protein_id="NP_014252.2"
/db_xref="GeneID:855575"
/db_xref="SGD:S000005091"
/translation="MHQQHSKSENKPQQQRKKFEGPKREAILDLAKYKDSKIRVKLMG
GKLVIGVLKGYDQLMNLVLDDTVEYMSNPDDENNTELISKNARKLGLTVIRGTILVSL
SSAEGSDVLYMQK"
gene complement(<351386..>351580)
/locus_tag="YNL146C-A"
/db_xref="GeneID:1466510"
mRNA complement(<351386..>351580)
/locus_tag="YNL146C-A"
/product="uncharacterized protein"
/transcript_id="NM_001184663.1"
/db_xref="GeneID:1466510"
CDS complement(351386..351580)
/locus_tag="YNL146C-A"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878152.1"
/db_xref="GeneID:1466510"
/db_xref="SGD:S000028851"
/translation="MISVCFVFPHSLALDFKSRCKKNRTKLCSAYYVSQVLRICKEMP
YRDLILFSTVRKGVYMRLYY"
gene <351715..>352017
/locus_tag="YNL146W"
/db_xref="GeneID:855576"
mRNA <351715..>352017
/locus_tag="YNL146W"
/product="uncharacterized protein"
/transcript_id="NM_001182984.3"
/db_xref="GeneID:855576"
CDS 351715..352017
/locus_tag="YNL146W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762|PMID:14562095]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to the endoplasmic
reticulum; YNL146W is not an essential gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014253.3"
/db_xref="GeneID:855576"
/db_xref="SGD:S000005090"
/translation="MSNTKHTTSHHMELKRIIILTLLFILIMLIFRNSVSFKMTFQEL
LPRFYKKNSNSVSNNNRPSSIFSENLVDFDDVNMVDKTRLFIFLFFSFIITIPFMV"
gene <352414..>352530
/gene="MFA2"
/locus_tag="YNL145W"
/db_xref="GeneID:855577"
mRNA <352414..>352530
/gene="MFA2"
/locus_tag="YNL145W"
/product="mating pheromone a"
/transcript_id="NM_001182983.1"
/db_xref="GeneID:855577"
CDS 352414..352530
/gene="MFA2"
/locus_tag="YNL145W"
/experiment="EXISTENCE:direct assay:GO:0005576
extracellular region [PMID:2046670]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0000750
pheromone-dependent signal transduction involved in
conjugation with cellular fusion [PMID:3285180]"
/experiment="EXISTENCE:mutant phenotype:GO:0000772 mating
pheromone activity [PMID:3285180]"
/note="Mating pheromone a-factor; made by a cells;
interacts with alpha cells to induce cell cycle arrest and
other responses leading to mating; biogenesis involves
C-terminal modification, N-terminal proteolysis, and
export; also encoded by MFA1"
/codon_start=1
/product="mating pheromone a"
/protein_id="NP_014254.1"
/db_xref="GeneID:855577"
/db_xref="SGD:S000005089"
/translation="MQPITTASTQATQKDKSSEKKDNYIIKGLFWDPACVIA"
gene complement(<352820..>355042)
/gene="MPF1"
/locus_tag="YNL144C"
/db_xref="GeneID:855578"
mRNA complement(<352820..>355042)
/gene="MPF1"
/locus_tag="YNL144C"
/product="Mpf1p"
/transcript_id="NM_001182982.1"
/db_xref="GeneID:855578"
CDS complement(352820..355042)
/gene="MPF1"
/locus_tag="YNL144C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0032473 cytoplasmic
side of mitochondrial outer membrane [PMID:40175596]"
/note="hypothetical protein; involved in the dual
distribution of tail-anchored proteins such as Fis1p and
Gem1p to mitochondria and peroxisomes; unstable protein
that associates with the cytosolic face of the
mitochondrial outer membrane; detected in highly purified
mitochondria in high-throughput studies; contains a PH
domain that impacts protein stability; binds
phosphatidylinositols and phosphatidylethanolamine in a
large-scale study"
/codon_start=1
/product="Mpf1p"
/protein_id="NP_014255.1"
/db_xref="GeneID:855578"
/db_xref="SGD:S000005088"
/translation="MSSSIFEMTIDHDGMDSTGLKLSQTASSISMGDEFLCSSTTSNS
ILDSPLPKVTFNHIDSITDINTNIMNEIVEPQSGVDVDVADKNVLYCIDPYPVEPPCY
DFANPSKVIRYPIYEHCRPCLTSVKPPSYTPSVEHYTVVSMKMEKLSPFENASSRLWN
NFILQINSTQINFYSIDDSLTRHIKNYRGGDMFDHSHHSKTASDRHHSARSLLNAFTT
KSTYQFDKYDKERICGEIARDEHKFLSDERLFKSYSLQCAKVGLPIDYSSRDFVLRMR
CEGQQFLVQFSHVDELIYWAMYLNMGISLSLDLELREMPTYRSVPRRRHPRARRFKQH
NKNKNKNKSRQNSDRNDSRSHSLLLRRSHTSSVVTKVATGNERPTNKSRSRSLSLLPP
SVSDASHDSNGPNDSGSLAGESSQTDFCGLFTSKLRNFFKTDSSSRKNSNMDIGQKMR
SKELNSVQEEIDDNGSTTNTNTSVLSSTFSPTAHSVLTAQTSIHENFRSRSNSNPIDP
LRCDRSVLKINNFEPVYEGTGRSTISNSAERTNREEKLNRGNEDVNGDEDDDEDEDDA
NYEEDDEDGYVEDDDINRLVYLDERESRYENADVEYGTIFSTKSFSVTGCLNHDFPKK
LSLKCGNKNQSSNDSKWAPATQLVSRKRYIKDSLRCIKPLTEDHPWVGKIIFKPALPP
AFETNNPPIRVYSGEDSTDLMHVKNHYLKPYIVGSCGFLKTGSKLFHFYDRTNDLTNL
"
gene complement(<356794..>357186)
/locus_tag="YNL143C"
/db_xref="GeneID:855579"
mRNA complement(<356794..>357186)
/locus_tag="YNL143C"
/product="uncharacterized protein"
/transcript_id="NM_001270759.1"
/db_xref="GeneID:855579"
CDS complement(356794..357186)
/locus_tag="YNL143C"
/note="hypothetical protein; expressed at both mRNA and
protein levels"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001257688.1"
/db_xref="GeneID:855579"
/db_xref="SGD:S000005087"
/translation="MREQLKLFTREIVDFTFLILSGFDYYQTLLISSNSSKKRPKDSS
LLSEKKKKKKKKKKDVLSYLSYLKDLPFVPFLFWQPGYSQREKNPRQHSLFIMTITKP
GMISMADMNYVVSKNRSLNRPAERGGNR"
gene <357453..>358952
/gene="MEP2"
/locus_tag="YNL142W"
/db_xref="GeneID:855580"
mRNA <357453..>358952
/gene="MEP2"
/locus_tag="YNL142W"
/product="ammonium permease MEP2"
/transcript_id="NM_001182980.1"
/db_xref="GeneID:855580"
CDS 357453..358952
/gene="MEP2"
/locus_tag="YNL142W"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:24124599|PMID:11069679]"
/experiment="EXISTENCE:direct assay:GO:0008519 ammonium
channel activity [PMID:9234685]"
/experiment="EXISTENCE:mutant phenotype:GO:0007124
pseudohyphal growth [PMID:9482721]"
/experiment="EXISTENCE:mutant phenotype:GO:0008519
ammonium channel activity [PMID:9234685]"
/experiment="EXISTENCE:mutant phenotype:GO:0019740
nitrogen utilization [PMID:9234685]"
/experiment="EXISTENCE:mutant phenotype:GO:0072488
ammonium transmembrane transport [PMID:9234685]"
/experiment="EXISTENCE:mutant phenotype:GO:1900430
positive regulation of filamentous growth of a population
of unicellular organisms [PMID:32069286]"
/note="Ammonium permease involved in regulation of
pseudohyphal growth; belongs to Mep-Amt-Rh family of
well-conserved ammonium (NH4+) transporters that includes
human Rh factors; expression is under nitrogen catabolite
repression regulation; activity is controlled by
phospho-silencing; phosphorylation of Mep2 mediated by
Npr1; dephosphorylation involves Psr1p and Psr2p; Twin-His
motif ensures transport specificity"
/codon_start=1
/product="ammonium permease MEP2"
/protein_id="NP_014257.1"
/db_xref="GeneID:855580"
/db_xref="SGD:S000005086"
/translation="MSYNFTGTPTGEGTGGNSLTTDLNTQFDLANMGWIGVASAGVWI
MVPGIGLLYSGLSRKKHALSLLWASMMASAVCIFQWFFWGYSLAFSHNTRGNGFIGTL
EFFGFRNVLGAPSSVSSLPDILFAVYQGMFAAVTGALMLGGACERARLFPMMVFLFLW
MTIVYCPIACWVWNAEGWLVKLGSLDYAGGLCVHLTSGHGGLVYALILGKRNDPVTRK
GMPKYKPHSVTSVVLGTVFLWFGWMFFNGGSAGNATIRAWYSIMSTNLAAACGGLTWM
VIDYFRCGRKWTTVGLCSGIIAGLVGITPAAGFVPIWSAVVIGVVTGAGCNLAVDLKS
LLRIDDGLDCYSIHGVGGCIGSVLTGIFAADYVNATAGSYISPIDGGWINHHYKQVGY
QLAGICAALAWTVTVTSILLLTMNAIPFLKLRLSADEEELGTDAAQIGEFTYEESTAY
IPEPIRSKTSAQMPPPHENIDDKIVGNTDAEKNSTPSDASSTKNTDHIV"
gene <359596..>360639
/gene="AAH1"
/locus_tag="YNL141W"
/db_xref="GeneID:855581"
mRNA <359596..>360639
/gene="AAH1"
/locus_tag="YNL141W"
/product="adenine deaminase"
/transcript_id="NM_001182979.1"
/db_xref="GeneID:855581"
CDS 359596..360639
/gene="AAH1"
/locus_tag="YNL141W"
/EC_number="3.5.4.2"
/experiment="EXISTENCE:direct assay:GO:0000034 adenine
deaminase activity [PMID:1577682]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006146 adenine
catabolic process [PMID:1577682]"
/experiment="EXISTENCE:direct assay:GO:0043101
purine-containing compound salvage [PMID:1577682]"
/experiment="EXISTENCE:direct assay:GO:0043103
hypoxanthine salvage [PMID:1577682]"
/experiment="EXISTENCE:mutant phenotype:GO:0000034 adenine
deaminase activity [PMID:1577682]"
/experiment="EXISTENCE:mutant phenotype:GO:0006146 adenine
catabolic process [PMID:1577682]"
/experiment="EXISTENCE:mutant phenotype:GO:0043101
purine-containing compound salvage [PMID:1577682]"
/experiment="EXISTENCE:mutant phenotype:GO:0043103
hypoxanthine salvage [PMID:1577682]"
/note="Adenine deaminase (adenine aminohydrolase);
converts adenine to hypoxanthine; involved in purine
salvage; transcriptionally regulated by nutrient levels
and growth phase; Aah1p degraded upon entry into
quiescence via SCF and the proteasome"
/codon_start=1
/product="adenine deaminase"
/protein_id="NP_014258.1"
/db_xref="GeneID:855581"
/db_xref="SGD:S000005085"
/translation="MVSVEFLQELPKCEHHLHLEGTLEPDLLFPLAKRNDIILPEGFP
KSVEELNEKYKKFRDLQDFLDYYYIGTNVLISEQDFFDLAWAYFKKVHKQGLVHAEVF
YDPQSHTSRGISIETVTKGFQRACDKAFSEFGITSKLIMCLLRHIEPEECLKTIEEAT
PFIKDGTISALGLDSAEKPFPPHLFVECYGKAASLNKDLKLTAHAGEEGPAQFVSDAL
DLLQVTRIDHGINSQYDEELLDRLSRDQTMLTICPLSNVKLQVVQSVSELPLQKFLDR
DVPFSLNSDDPAYFGGYILDVYTQVSKDFPHWDHETWGRIAKNAIKGSWCDDKRKNGL
LSRVDEVVTKYSH"
gene complement(<360917..>361486)
/locus_tag="YNL140C"
/db_xref="GeneID:855582"
mRNA complement(<360917..>361486)
/locus_tag="YNL140C"
/product="uncharacterized protein"
/transcript_id="NM_001270760.1"
/db_xref="GeneID:855582"
CDS complement(360917..361486)
/locus_tag="YNL140C"
/note="hypothetical protein; expressed at both mRNA and
protein levels; partially overlaps THO2/YNL139C"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001257689.1"
/db_xref="GeneID:855582"
/db_xref="SGD:S000005084"
/translation="MLEAKMVMVVPTIEDQAMIVLHPKILSQSVVMHIKGQNYQLDLV
KVKHIMIEVGHLDPQVRTGAMVLIKGITAYVKSIRKTVHNVHNYGFLKSHFKRAKTAA
KPTLTKLHLTSATRLQKMKKSRIKDSRRMKQLETNFKRKIIEIHEIAVLPIEPMKIKG
TTEIERAILRRFRKVPRVGITSVGTRGNY"
gene complement(<360924..>365717)
/gene="THO2"
/locus_tag="YNL139C"
/gene_synonym="LDB5; RLR1; ZRG13"
/db_xref="GeneID:855583"
mRNA complement(<360924..>365717)
/gene="THO2"
/locus_tag="YNL139C"
/gene_synonym="LDB5; RLR1; ZRG13"
/product="Tho2p"
/transcript_id="NM_001182977.3"
/db_xref="GeneID:855583"
CDS complement(360924..365717)
/gene="THO2"
/locus_tag="YNL139C"
/gene_synonym="LDB5; RLR1; ZRG13"
/experiment="EXISTENCE:direct assay:GO:0000445 THO complex
part of transcription export complex [PMID:11979277]"
/experiment="EXISTENCE:direct assay:GO:0000781 chromosome,
telomeric region [PMID:24084588]"
/experiment="EXISTENCE:direct assay:GO:0003676 nucleic
acid binding [PMID:12093753]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11139493|PMID:22842922]"
/experiment="EXISTENCE:mutant phenotype:GO:0000446
nucleoplasmic THO complex [PMID:11060033]"
/experiment="EXISTENCE:mutant phenotype:GO:0006283
transcription-coupled nucleotide-excision repair
[PMID:17537816]"
/experiment="EXISTENCE:mutant phenotype:GO:0006310 DNA
recombination [PMID:11060033]"
/experiment="EXISTENCE:mutant phenotype:GO:0006368
transcription elongation by RNA polymerase II
[PMID:17960421|PMID:12093753]"
/experiment="EXISTENCE:mutant phenotype:GO:0006406 mRNA
export from nucleus [PMID:11979277]"
/experiment="EXISTENCE:mutant phenotype:GO:0031124 mRNA
3'-end processing [PMID:18614048]"
/experiment="EXISTENCE:mutant phenotype:GO:0034063 stress
granule assembly [PMID:27251550]"
/experiment="EXISTENCE:mutant phenotype:GO:0045943
positive regulation of transcription by RNA polymerase I
[PMID:26663077]"
/experiment="EXISTENCE:mutant phenotype:GO:0097185
cellular response to azide [PMID:27251550]"
/experiment="EXISTENCE:mutant phenotype:GO:2001209
positive regulation of transcription elongation by RNA
polymerase I [PMID:26663077]"
/note="Subunit of the THO complex; THO is required for
efficient transcription elongation and involved in
transcriptional elongation-associated recombination;
required for LacZ RNA expression from certain plasmids"
/codon_start=1
/product="Tho2p"
/protein_id="NP_014260.3"
/db_xref="GeneID:855583"
/db_xref="SGD:S000005083"
/translation="MAEQTLLSKLNALSQKVIPPASPSQASILTEEVIRNWPERSKTL
CSDFTALESNDEKEDWLRTLFIELFDFINKNDENSPLKLSDVASFTNELVNHERQVSQ
ASIVGKMFIAVSSTVPNINDLTTISLCKLIPSLHEELFKFSWISSKLLNKEQTTLLRH
LLKKSKYELKKYNLLVENSVGYGQLVALLILAYYDPDNFSKVSAYLKEIYHIMGKYSL
DSIRTLDVILNVSSQFITEGYKFFIALLRKSDSWPSSHVANNSNYSSLNEGGNMIAAN
IISFNLSQYNEEVDKENYERYMDMCCILLKNGFVNFYSIWDNVKPEMEFLQEYIQNLE
TELEEESTKGVENPLAMAAALSTENETDEDNALVVNDDVNMKDKISEETNADIESKGK
QKTQQDILLFGKIKLLERLLIHGCVIPVIHVLKQYPKVLYVSESLSRYLGRVFEYLLN
PLYTSMTSSGESKDMATALMITRIDNGILAHKPRLIHKYKTHEPFESLELNSSYVFYY
SEWNSNLTPFASVNDLFENSHIYLSIIGPYLGRIPTLLSKISRIGVADIQKNHGSESL
HVTIDKWIDYVRKFIFPATSLLQNNPIATSEVYELMKFFPFEKRYFIYNEMMTKLSQD
ILPLKVSFNKAEREAKSILKALSIDTIAKESRRFAKLISTNPLASLVPAVKQIENYDK
VSELVVYTTKYFNDFAYDVLQFVLLLRLTYNRPAVQFDGVNQAMWVQRLSIFIAGLAK
NCPNMDISNIITYILKTLHNGNIIAVSILKELIITVGGIRDLNEVNMKQLLMLNSGSP
LKQYARHLIYDFRDDNSVISSRLTSFFTDQSAISEIILLLYTLNLKANTQNSHYKILS
TRCDEMNTLLWSFIELIKHCLKGKAFEENVLPFVELNNRFHLSTPWTFHIWRDYLDNQ
LNSNENFSIDELIEGAEFSDVDLTKISKDLFTTFWRLSLYDIHFDKSLYDERKNALSG
ENTGHMSNRKKHLIQNQIKDILVTGISHQRAFKKTSEFISEKSNVWNKDCGEDQIKIF
LQNCVVPRVLFSPSDALFSSFFIFMAFRTENLMSILNTCITSNILKTLLFCCTSSEAG
NLGLFFTDVLKKLEKMRLNGDFNDQASRKLYEWHSVITEQVIDLLSEKNYMSIRNGIE
FMKHVTSVFPVVKAHIQLVYTTLEENLINEEREDIKLPSSALIGHLKARLKDALELDE
FCTLTEEEAEQKRIREMELEEIKNYETACQNEQKQVALRKQLELNKSQRLQNDPPKSV
ASGSAGLNSKDRYTYSRNEPVIPTKPSSSQWSYSKVTRHVDDINHYLATNHLQKAISL
VENDDETRNLRKLSKQNMPIFDFRNSTLEIFERYFRTLIQNPQNPDFAEKIDSLKRYI
KNISREPYPDTTSSYSEAAAPEYTKRSSRYSGNAGGKDGYGSSNYRGPSNDRSAPKNI
KPISSYAHKRSELPTRPSKSKTYNDRSRALRPTGPDRGDGFDQRDNRLREEYKKNSSQ
RSQLRFPEKPFQEGKDSSKANPYQASSYKRDSPSENEEKPNKRFKKDETIRNKFQTQD
YRNTRDSGAAHRANENQRYNGNRKSNTQALPQGPKGGNYVSRYQR"
gene <366033..>366412
/gene="YSF3"
/locus_tag="YNL138W-A"
/gene_synonym="RCP10"
/db_xref="GeneID:1466511"
mRNA join(<366033..366035,366158..>366412)
/gene="YSF3"
/locus_tag="YNL138W-A"
/gene_synonym="RCP10"
/product="U2 snRNP complex subunit YSF3"
/transcript_id="NM_001184519.1"
/db_xref="GeneID:1466511"
CDS join(366033..366035,366158..366412)
/gene="YSF3"
/locus_tag="YNL138W-A"
/gene_synonym="RCP10"
/experiment="EXISTENCE:direct assay:GO:0005686 U2 snRNP
[PMID:16314500|PMID:15565172]"
/experiment="EXISTENCE:mutant phenotype:GO:0000245
spliceosomal complex assembly [PMID:16314500]"
/note="Component of the SF3b subcomplex of the U2 snRNP;
essential protein required for splicing and for assembly
of SF3b"
/codon_start=1
/product="U2 snRNP complex subunit YSF3"
/protein_id="NP_878153.1"
/db_xref="GeneID:1466511"
/db_xref="SGD:S000028509"
/translation="MAEKQRQLKLQKIYKQKYIGLGDESTTREQWQRNVRNDTLNTLQ
GHSASLEYVSLSRGDLSIRDTRIHLLKSMSPGYKAYLREER"
gene <366741..>368321
/gene="SRV2"
/locus_tag="YNL138W"
/gene_synonym="CAP"
/db_xref="GeneID:855584"
mRNA <366741..>368321
/gene="SRV2"
/locus_tag="YNL138W"
/gene_synonym="CAP"
/product="adenylate cyclase-binding protein"
/transcript_id="NM_001182976.1"
/db_xref="GeneID:855584"
CDS 366741..368321
/gene="SRV2"
/locus_tag="YNL138W"
/gene_synonym="CAP"
/experiment="EXISTENCE:direct assay:GO:0003779 actin
binding [PMID:19201756]"
/experiment="EXISTENCE:direct assay:GO:0007015 actin
filament organization [PMID:19201756]"
/experiment="EXISTENCE:direct assay:GO:0008179 adenylate
cyclase binding [PMID:2184942]"
/experiment="EXISTENCE:direct assay:GO:0030042 actin
filament depolymerization [PMID:26458246]"
/experiment="EXISTENCE:direct assay:GO:0030479 actin
cortical patch [PMID:8552082]"
/experiment="EXISTENCE:direct assay:GO:0030836 positive
regulation of actin filament depolymerization
[PMID:26458246]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807]"
/experiment="EXISTENCE:direct assay:GO:0051014 actin
filament severing [PMID:23135996]"
/experiment="EXISTENCE:mutant phenotype:GO:0007015 actin
filament organization [PMID:19201756]"
/experiment="EXISTENCE:mutant phenotype:GO:0007265 Ras
protein signal transduction [PMID:9774417]"
/experiment="EXISTENCE:mutant phenotype:GO:0030833
regulation of actin filament polymerization
[PMID:30639852]"
/experiment="EXISTENCE:mutant phenotype:GO:0090141
positive regulation of mitochondrial fission
[PMID:30639852]"
/note="Cyclase-associated protein, CAP; accelerates
nucleotide exchange and actin filament depolymerization;
N-terminus binds adenylate cyclase, facilitating
activation by RAS and directly catalyzes cofilin-mediated
severing of actin filaments; C-terminus binds and recycles
cofilin bound, ADP-actin monomers, facilitating regulation
of actin dynamics and cell morphogenesis; processively
tracks and promotes depolymerization of actin filament
barbed and pointed ends; mCAP1 is the mouse homolog"
/codon_start=1
/product="adenylate cyclase-binding protein"
/protein_id="NP_014261.1"
/db_xref="GeneID:855584"
/db_xref="SGD:S000005082"
/translation="MPDSKYTMQGYNLVKLLKRLEEATARLEDVTIYQEGYIQNKLEA
SKNNKPSDSGADANTTNEPSAENAPEVEQDPKCITAFQSYIGENIDPLVELSGKIDTV
VLDALQLLKGGFQSQLTFLRAAVRSRKPDYSSQTFADSLRPINENIIKLGQLKESNRQ
SKYFAYLSALSEGAPLFSWVAVDTPVSMVTDFKDAAQFWTNRILKEYRESDPNAVEWV
KKFLASFDNLKAYIKEYHTTGVSWKKDGMDFADAMAQSTKNTGATSSPSPASATAAPA
PPPPPPAPPASVFEISNDTPATSSDANKGGIGAVFAELNQGENITKGLKKVDKSQQTH
KNPELRQSSTVSSTGSKSGPPPRPKKPSTLKTKRPPRKELVGNKWFIENYENETESLV
IDANKDESIFIGKCSQVLVQIKGKVNAISLSETESCSVVLDSSISGMDVIKSNKFGIQ
VNHSLPQISIDKSDGGNIYLSKESLNTEIYTSCSTAINVNLPIGEDDDYVEFPIPEQM
KHSFADGKFKSAVFEHAG"
gene complement(<368595..>370055)
/gene="NAM9"
/locus_tag="YNL137C"
/gene_synonym="MNA6; uS4m"
/db_xref="GeneID:855585"
mRNA complement(<368595..>370055)
/gene="NAM9"
/locus_tag="YNL137C"
/gene_synonym="MNA6; uS4m"
/product="mitochondrial 37S ribosomal protein uS4m NAM9"
/transcript_id="NM_001182975.3"
/db_xref="GeneID:855585"
CDS complement(368595..370055)
/gene="NAM9"
/locus_tag="YNL137C"
/gene_synonym="MNA6; uS4m"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:11278769]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:11278769]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005763
mitochondrial small ribosomal subunit [PMID:11278769]"
/note="Mitochondrial ribosomal component of the small
subunit"
/codon_start=1
/product="mitochondrial 37S ribosomal protein uS4m NAM9"
/protein_id="NP_014262.3"
/db_xref="GeneID:855585"
/db_xref="SGD:S000005081"
/translation="MPRKANLLKSLARGRVRTSFNKYNLFNLYKKGGVDLKSKSLYQQ
KWTAKQETRAYHGEHLTEKRWQTVFKPKLDSVAQLDASLRGGEIKETPFLLQTFAVLE
KRLDFALFRAMFASSVRQARQFILHGNVRVNGVKIKHPSYTLKPGDMFSVKPDKVLEA
LGAKKPSFQEALKIDKTQIVLWNKYVKEAKTEPKEVWEKKLENFEKMSDSNPKKLQFQ
EFLRQYNKNLESQQYNALKGCTQEGILRKLLNVEKEIGKSNNEPLSIDELKQGLPEIQ
DSQLLESLNNAYQEFFKSGEIRREIISKCQPDELISLATEMMNPNETTKKELSDGAKS
ALRSGKRIIAESVKLWTKNITDHFKTRMSDISDGSLTFDPKWAKNLKYHDPIKLSELE
GDEPKARKLINLPWQKNYVYGRQDPKKPFFTPWKPRPFLSPFAILPHHLEISFKTCHA
VYLRDPVARPGQSEVISPFDVPVHERAYMYYLRNGK"
gene <370368..>371645
/gene="EAF7"
/locus_tag="YNL136W"
/db_xref="GeneID:855586"
mRNA <370368..>371645
/gene="EAF7"
/locus_tag="YNL136W"
/product="Eaf7p"
/transcript_id="NM_001182974.1"
/db_xref="GeneID:855586"
CDS 370368..371645
/gene="EAF7"
/locus_tag="YNL136W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006281 DNA repair
[PMID:16135807]"
/experiment="EXISTENCE:direct assay:GO:0035267 NuA4
histone acetyltransferase complex [PMID:15353583]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:15353583]"
/experiment="EXISTENCE:physical interaction:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:15353583]"
/note="Subunit of the NuA4 histone acetyltransferase
complex; NuA4 acetylates the N-terminal tails of histones
H4 and H2A"
/codon_start=1
/product="Eaf7p"
/protein_id="NP_014263.1"
/db_xref="GeneID:855586"
/db_xref="SGD:S000005080"
/translation="MVVHWTIVDEIRLLRWASEFKPAGIHKHFHMFCIVERMNSPDKY
PVTLLQKETMKLGKVFTAKDIWDKLSQSYNLEKIDEMENTYSLEATTESSRNGNGNGD
DAEIHEETLLELNNRIRVRKQDFTLPWEEYGELILENARKSPNSNEEYPRVEDMNEKD
STIPKESPSTDLKNDNNKQEKNATIKVKELPEYHTEENDSPIDVQKEPIKEVQSDEKE
LQREHMSEEEQKMKSTNKTAAPVRKSQRLKRSKEVKFEDEEKEEIEEDNTKDEEQKEK
KEEIQEPKITHNEEVDKEKNENEEGDDEREKSTSYENTNGSESEGVDEGVDEELGYES
EREAEGKGKQIESEGGNLKKKTENKKGDDQQDDTKKDSKDKNEPLAKRTRHSSSTGNT
SNETSPKRKRRKAGSRKNSPPATRVSSRLRNKK"
gene complement(<371882..>372226)
/gene="FPR1"
/locus_tag="YNL135C"
/gene_synonym="FKB1; RBP1"
/db_xref="GeneID:855587"
mRNA complement(<371882..>372226)
/gene="FPR1"
/locus_tag="YNL135C"
/gene_synonym="FKB1; RBP1"
/product="peptidylprolyl isomerase FPR1"
/transcript_id="NM_001182973.1"
/db_xref="GeneID:855587"
CDS complement(371882..372226)
/gene="FPR1"
/locus_tag="YNL135C"
/gene_synonym="FKB1; RBP1"
/EC_number="5.2.1.8"
/experiment="EXISTENCE:direct assay:GO:0003755
peptidyl-prolyl cis-trans isomerase activity
[PMID:1996117]"
/experiment="EXISTENCE:direct assay:GO:0005515 protein
binding [PMID:36113412]"
/experiment="EXISTENCE:direct assay:GO:0005527 macrolide
binding [PMID:8325502]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:36113412|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0044183 protein
folding chaperone [PMID:34534579]"
/experiment="EXISTENCE:direct assay:GO:0070651
nonfunctional rRNA decay [PMID:36113412]"
/experiment="EXISTENCE:direct assay:GO:1903332 regulation
of protein folding [PMID:34534579]"
/experiment="EXISTENCE:genetic interaction:GO:0006325
chromatin organization [PMID:10049913]"
/experiment="EXISTENCE:genetic interaction:GO:1901710
regulation of homoserine biosynthetic process
[PMID:15470257]"
/experiment="EXISTENCE:mutant phenotype:GO:0001228
DNA-binding transcription activator activity, RNA
polymerase II-specific [PMID:32603360]"
/experiment="EXISTENCE:mutant phenotype:GO:0006366
transcription by RNA polymerase II [PMID:32603360]"
/experiment="EXISTENCE:mutant phenotype:GO:0006457 protein
folding [PMID:9362068]"
/note="Peptidyl-prolyl cis-trans isomerase (PPIase); binds
to FK506 and rapamycin; also binds to the nonhistone
chromatin binding protein Hmo1p and may regulate its
assembly or function; associates with promoters of
ribosomal protein genes; acts as chaperone to prevent
protein aggregation; N-terminally propionylated in vivo;
mutation is functionally complemented by human FKBP1A"
/codon_start=1
/product="peptidylprolyl isomerase FPR1"
/protein_id="NP_014264.1"
/db_xref="GeneID:855587"
/db_xref="SGD:S000005079"
/translation="MSEVIEGNVKIDRISPGDGATFPKTGDLVTIHYTGTLENGQKFD
SSVDRGSPFQCNIGVGQVIKGWDVGIPKLSVGEKARLTIPGPYAYGPRGFPGLIPPNS
TLVFDVELLKVN"
gene complement(<372451..>373581)
/locus_tag="YNL134C"
/db_xref="GeneID:855588"
mRNA complement(<372451..>373581)
/locus_tag="YNL134C"
/product="uncharacterized protein"
/transcript_id="NM_001182972.3"
/db_xref="GeneID:855588"
CDS complement(372451..373581)
/locus_tag="YNL134C"
/experiment="EXISTENCE:direct assay:GO:0004029 aldehyde
dehydrogenase (NAD+) activity [PMID:25656244]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:1901426
response to furfural [PMID:25656244]"
/note="NADH-dependent aldehyde reductase; involved in
detoxification of furfural; expression is up-regulated in
the presence of furfural and 5-hydroxymethylfurfural,
which are compounds generated during lignocellulosic
biomass pre-treatment; green fluorescent protein
(GFP)-fusion protein localizes to both the cytoplasm and
nucleus; protein abundance increases in response to DNA
replication stress"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014265.3"
/db_xref="GeneID:855588"
/db_xref="SGD:S000005078"
/translation="MSASIPETMKAVVIENGKAVVKQDIPIPELEEGFVLIKTVAVAG
NPTDWKHIDFKIGPQGALLGCDAAGQIVKLGPNVDAARFAIGDYIYGVIHGASVRFPS
NGAFAEYSAISSETAYKPAREFRLCGKDKLPEGPVKSLEGAVSLPVSLTTAGMILTHS
FGLDMTWKPSKAQRDQPILFWGGATAVGQMLIQLAKKLNGFSKIIVVASRKHEKLLKE
YGADELFDYHDADVIEQIKKKYNNIPYLVDCVSNTETIQQVYKCAADDLDATVVQLTV
LTEKDIKEEDRRQNVSIEGTLLYLIGGNDVPFGTFTLPADPEYKEAAIKFIKFINPKI
NDGEIHHIPVKVYKNGLDDIPQLLDDIKHGRNSGEKLVAVLK"
gene complement(<374171..>374692)
/gene="FYV6"
/locus_tag="YNL133C"
/db_xref="GeneID:855589"
mRNA complement(<374171..>374692)
/gene="FYV6"
/locus_tag="YNL133C"
/product="Fyv6p"
/transcript_id="NM_001182971.3"
/db_xref="GeneID:855589"
CDS complement(374171..374692)
/gene="FYV6"
/locus_tag="YNL133C"
/experiment="EXISTENCE:direct assay:GO:0005198 structural
molecule activity [PMID:35705093]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0005198
structural molecule activity [PMID:12399380]"
/experiment="EXISTENCE:mutant phenotype:GO:0006303
double-strand break repair via nonhomologous end joining
[PMID:12399380]"
/note="Splicing factor, structural and functional homolog
of human FAM192A; plays structural role in intermediate
spliceosomal C* complex, which executes exon ligation;
required for survival upon exposure to K1 killer toxin;
proposed to regulate double-strand break repair via
non-homologous end-joining"
/codon_start=1
/product="Fyv6p"
/protein_id="NP_014266.3"
/db_xref="GeneID:855589"
/db_xref="SGD:S000005077"
/translation="MSSTSDNNANSAREKKPLKFVSEGVGNVEAQRIREQVEQKKYEA
EYKRKTRKSLRDQLRSNAISKQKQYNGLVRDRESFTRLSKEDLEFYQKSKNELLKKEK
ELNNYLDVKAINFEKKKKALLMENDSTTNTEKYLETGTSLGSKTQIKGVKTSSPKPKI
KVSIKKLGRKLEN"
gene 374869..374959
/locus_tag="YNCN0006W"
/db_xref="GeneID:855590"
tRNA join(374869..374905,374924..374959)
/locus_tag="YNCN0006W"
/product="tRNA-Phe"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Phenylalanine tRNA (tRNA-Phe), predicted by
tRNAscan-SE analysis"
/db_xref="GeneID:855590"
/db_xref="SGD:S000006569"
gene <375321..>378491
/gene="KRE33"
/locus_tag="YNL132W"
/gene_synonym="RRA1"
/db_xref="GeneID:855591"
mRNA <375321..>378491
/gene="KRE33"
/locus_tag="YNL132W"
/gene_synonym="RRA1"
/product="ribosome biosynthesis protein KRE33"
/transcript_id="NM_001182970.1"
/db_xref="GeneID:855591"
CDS 375321..378491
/gene="KRE33"
/locus_tag="YNL132W"
/gene_synonym="RRA1"
/experiment="EXISTENCE:direct assay:GO:0000049 tRNA
binding [PMID:28542199]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0030515 snoRNA
binding [PMID:28542199]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/experiment="EXISTENCE:direct assay:GO:0051391 tRNA
acetylation [PMID:38613394]"
/experiment="EXISTENCE:direct assay:GO:1990883 18S rRNA
cytidine N-acetyltransferase activity [PMID:25086048]"
/experiment="EXISTENCE:mutant phenotype:GO:0016556 mRNA
modification [PMID:31243956]"
/experiment="EXISTENCE:mutant phenotype:GO:0042274
ribosomal small subunit biogenesis
[PMID:19806183|PMID:12150911]"
/note="Acetyltransferase required for biogenesis of small
ribosomal subunit; conserved 18S rRNA cytosine
acetyltransferase that modifies tRNAs assisted by adaptor
Tan1p; responsible for incorporation of N4-acetylcytidine
into mRNAs; human homolog NAT10 implicated in several
types of cancer and premature aging"
/codon_start=1
/product="ribosome biosynthesis protein KRE33"
/protein_id="NP_014267.1"
/db_xref="GeneID:855591"
/db_xref="SGD:S000005076"
/translation="MAKKAIDSRIPSLIRNGVQTKQRSIFVIVGDRARNQLPNLHYLM
MSADLKMNKSVLWAYKKKLLGFTSHRKKRENKIKKEIKRGTREVNEMDPFESFISNQN
IRYVYYKESEKILGNTYGMCILQDFEALTPNLLARTIETVEGGGIVVILLKSMSSLKQ
LYTMTMDVHARYRTEAHGDVVARFNERFILSLGSNPNCLVVDDELNVLPLSGAKNVKP
LPPKEDDELPPKQLELQELKESLEDVQPAGSLVSLSKTVNQAHAILSFIDAISEKTLN
FTVALTAGRGRGKSAALGISIAAAVSHGYSNIFVTSPSPENLKTLFEFIFKGFDALGY
QEHIDYDIIQSTNPDFNKAIVRVDIKRDHRQTIQYIVPQDHQVLGQAELVVIDEAAAI
PLPIVKNLLGPYLVFMASTINGYEGTGRSLSLKLIQQLRNQNNTSGRESTQTAVVSRD
NKEKDSHLHSQSRQLREISLDEPIRYAPGDPIEKWLNKLLCLDVTLIKNPRFATRGTP
HPSQCNLFVVNRDTLFSYHPVSENFLEKMMALYVSSHYKNSPNDLQLMSDAPAHKLFV
LLPPIDPKDGGRIPDPLCVIQIALEGEISKESVRNSLSRGQRAGGDLIPWLISQQFQD
EEFASLSGARIVRIATNPEYASMGYGSRAIELLRDYFEGKFTDMSEDVRPKDYSIKRV
SDKELAKTNLLKDDVKLRDAKTLPPLLLKLSEQPPHYLHYLGVSYGLTQSLHKFWKNN
SFVPVYLRQTANDLTGEHTCVMLNVLEGRESNWLVEFAKDFRKRFLSLLSYDFHKFTA
VQALSVIESSKKAQDLSDDEKHDNKELTRTHLDDIFSPFDLKRLDSYSNNLLDYHVIG
DMIPMLALLYFGDKMGDSVKLSSVQSAILLAIGLQRKNIDTIAKELNLPSNQTIAMFA
KIMRKMSQYFRQLLSQSIEETLPNIKDDAIAEMDGEEIKNYNAAEALDQMEEDLEEAG
SEAVQAMREKQKELINSLNLDKYAINDNSEEWAESQKSLEIAAKAKGVVSLKTGKKRT
TEKAEDIYRQEMKAMKKPRKSKKAAN"
gene <378767..>379225
/gene="TOM22"
/locus_tag="YNL131W"
/gene_synonym="MAS17; MAS22; MOM22"
/db_xref="GeneID:855592"
mRNA <378767..>379225
/gene="TOM22"
/locus_tag="YNL131W"
/gene_synonym="MAS17; MAS22; MOM22"
/product="Tom22p"
/transcript_id="NM_001182969.1"
/db_xref="GeneID:855592"
CDS 378767..379225
/gene="TOM22"
/locus_tag="YNL131W"
/gene_synonym="MAS17; MAS22; MOM22"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane
[PMID:16689936|PMID:16407407]"
/experiment="EXISTENCE:direct assay:GO:0005742
mitochondrial outer membrane translocase complex
[PMID:9774667]"
/experiment="EXISTENCE:mutant phenotype:GO:0008320 protein
transmembrane transporter activity [PMID:10519552]"
/experiment="EXISTENCE:mutant phenotype:GO:0030150 protein
import into mitochondrial matrix
[PMID:9774667|PMID:10519552]"
/experiment="EXISTENCE:mutant phenotype:GO:0045040 protein
insertion into mitochondrial outer membrane
[PMID:12628251]"
/note="Component of the TOM (Translocase of Outer
Membrane) complex; responsible for initial import of
mitochondrially directed proteins; mediates interaction
between TOM and TIM complexes and acts as receptor for
precursor proteins; implicated in mitochondrial
accumulation of amyloid beta peptides via direct
interaction with amyloid beta peptide residues 25-42
followed by transfer to Tom40p and transport through the
TOM channel"
/codon_start=1
/product="Tom22p"
/protein_id="NP_014268.1"
/db_xref="GeneID:855592"
/db_xref="SGD:S000005075"
/translation="MVELTEIKDDVVQLDEPQFSRNQAIVEEKASATNNDVVDDEDDS
DSDFEDEFDENETLLDRIVALKDIVPPGKRQTISNFFGFTSSFVRNAFTKSGNLAWTL
TTTALLLGVPLSLSILAEQQLIEMEKTFDLQSDANNILAQGEKDAAATAN"
gene complement(<379558..>380831)
/gene="CPT1"
/locus_tag="YNL130C"
/db_xref="GeneID:855593"
mRNA complement(join(<379558..380689,380782..>380831))
/gene="CPT1"
/locus_tag="YNL130C"
/product="diacylglycerol cholinephosphotransferase"
/transcript_id="NM_001182968.3"
/db_xref="GeneID:855593"
CDS complement(join(379558..380689,380782..380831))
/gene="CPT1"
/locus_tag="YNL130C"
/EC_number="2.7.8.2"
/experiment="EXISTENCE:direct assay:GO:0004142
diacylglycerol cholinephosphotransferase activity
[PMID:3029130]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:3005242]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:8543066]"
/experiment="EXISTENCE:direct assay:GO:0006657 CDP-choline
pathway [PMID:3029130]"
/experiment="EXISTENCE:mutant phenotype:GO:0004142
diacylglycerol cholinephosphotransferase activity
[PMID:3029130]"
/experiment="EXISTENCE:mutant phenotype:GO:0006657
CDP-choline pathway [PMID:3029130]"
/note="Cholinephosphotransferase; required for
phosphatidylcholine biosynthesis and for
inositol-dependent regulation of EPT1 transcription; CPT1
has a paralog, EPT1, that arose from the whole genome
duplication"
/codon_start=1
/product="diacylglycerol cholinephosphotransferase"
/protein_id="NP_014269.4"
/db_xref="GeneID:855593"
/db_xref="SGD:S000005074"
/translation="MGFFIPQSSLGNLKLYKYQSDDRSFLSNHVLRPFWRKFATIFPL
WMAPNLVTLLGFCFIIFNVLTTLYYDPYFDQESPRWTYFSYAIGLFLYQTFDACDGMH
ARRTGQQGPLGELFDHCIDSINTTLSMIPVCSMTGMGYTYMTIFSQFAILCSFYLSTW
EEYHTHKLYLAEFCGPVEGIIVLCISFIAVGIYGPQTIWHTKVAQFSWQDFVFDVETV
HLMYAFCTGALIFNIVTAHTNVVRYYESQSTKSATPSKTAENISKAVNGLLPFFAYFS
SIFTLVLIQPSFISLALILSIGFSVAFVVGRMIIAHLTMQPFPMVNFPFLIPTIQLVL
YAFMVYVLDYQKGSIVSALVWMGLGLTLAIHGMFINDIIYDITTFLDIYALSIKHPKE
I"
gene complement(<381245..>381391)
/gene="DGR1"
/locus_tag="YNL130C-A"
/db_xref="GeneID:1466512"
mRNA complement(<381245..>381391)
/gene="DGR1"
/locus_tag="YNL130C-A"
/product="Dgr1p"
/transcript_id="NM_001184574.1"
/db_xref="GeneID:1466512"
CDS complement(381245..381391)
/gene="DGR1"
/locus_tag="YNL130C-A"
/note="hypothetical protein; dgr1 null mutant is resistant
to 2-deoxy-D-glucose"
/codon_start=1
/product="Dgr1p"
/protein_id="NP_878154.1"
/db_xref="GeneID:1466512"
/db_xref="SGD:S000028579"
/translation="MQVGFVSQTNCRSFPACIVFLFQMSQRQRSFNANLRVFKSKCKK
IYIG"
gene <381480..>382202
/gene="NRK1"
/locus_tag="YNL129W"
/db_xref="GeneID:855594"
mRNA <381480..>382202
/gene="NRK1"
/locus_tag="YNL129W"
/product="ribosylnicotinamide kinase"
/transcript_id="NM_001182967.1"
/db_xref="GeneID:855594"
CDS 381480..382202
/gene="NRK1"
/locus_tag="YNL129W"
/EC_number="2.7.1.22"
/EC_number="2.7.1.173"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0046495
nicotinamide riboside metabolic process [PMID:15137942]"
/experiment="EXISTENCE:direct assay:GO:0050262
ribosylnicotinamide kinase activity [PMID:15137942]"
/experiment="EXISTENCE:genetic interaction:GO:0009435 NAD+
biosynthetic process [PMID:17914902]"
/experiment="EXISTENCE:genetic interaction:GO:0034355 NAD+
biosynthetic process via the salvage pathway
[PMID:15137942]"
/experiment="EXISTENCE:genetic interaction:GO:0046495
nicotinamide riboside metabolic process [PMID:15137942]"
/note="Nicotinamide riboside kinase; catalyzes the
phosphorylation of nicotinamide riboside and nicotinic
acid riboside in salvage pathways for NAD+ biosynthesis"
/codon_start=1
/product="ribosylnicotinamide kinase"
/protein_id="NP_014270.1"
/db_xref="GeneID:855594"
/db_xref="SGD:S000005073"
/translation="MTSKKVILVALSGCSSSGKTTIAKLTASLFTKATLIHEDDFYKH
DNEVPVDAKYNIQNWDSPEALDFKLFGKELDVIKQTGKIATKLIHNNNVDDPFTKFHI
DRQVWDELKAKYDSINDDKYEVVIVDGFMIFNNTGISKKFDLKILVRAPYEVLKKRRA
SRKGYQTLDSFWVDPPYYFDEFVYESYRANHAQLFVNGDVEGLLDPRKSKNIKEFIND
DDTPIAKPLSWVCQEILKLCKD"
gene <382359..>383663
/gene="TEP1"
/locus_tag="YNL128W"
/db_xref="GeneID:855595"
mRNA <382359..>383663
/gene="TEP1"
/locus_tag="YNL128W"
/product="putative
phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase"
/transcript_id="NM_001182966.4"
/db_xref="GeneID:855595"
CDS 382359..383663
/gene="TEP1"
/locus_tag="YNL128W"
/EC_number="3.1.3.67"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:15913452]"
/experiment="EXISTENCE:mutant phenotype:GO:0030476
ascospore wall assembly [PMID:11070083]"
/note="PTEN homolog with no demonstrated inositol lipid
phosphatase activity; plays a role in normal sporulation;
homolog of human tumor suppressor gene PTEN/MMAC1/TEP1 and
fission yeast ptn1"
/codon_start=1
/product="putative
phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase"
/protein_id="NP_014271.3"
/db_xref="GeneID:855595"
/db_xref="SGD:S000005072"
/translation="MREEGSELEMEKGFLKWKPVNLMKKILSLPMKKTKNDIGLRLDI
SYILVNLIVCSYPVNTYPKLLYRNSLDDLILFLTVYHGKGNFRIFNFRGEKEDSDYKD
NDLIGIAAKFESKDFEIQELRSTLINDGKIPISPIDLETRTLVEEETNNVICERIGWL
DHFPPPFELLEEIVDGIENYLSVSKNRVAVLHCRMGKGRSGMITVAYLMKYLQCPLGE
ARLIFMQARFKYGMTNGVTIPSQLRYLRYHEFFITHEKAAQEGISNEAVKFKFKFRLA
KMTFLRPSSLITSESAIVTTKIQHYNDDRNALLTRKVVYSDIMAHECGGNMTFIFGRD
YLTLENDCRIEFTLGTSKSKAASSIISWTSCASCWLNIYLETLMHIIKDDSSPDYFQV
ERLKRDEMLGTTISWQELDGFGELSTHGLKLFQALKLEWEII"
gene <383987..>386848
/gene="FAR11"
/locus_tag="YNL127W"
/db_xref="GeneID:855596"
mRNA <383987..>386848
/gene="FAR11"
/locus_tag="YNL127W"
/product="Far11p"
/transcript_id="NM_001182965.3"
/db_xref="GeneID:855596"
CDS 383987..386848
/gene="FAR11"
/locus_tag="YNL127W"
/experiment="EXISTENCE:direct assay:GO:0000138 Golgi trans
cisterna [PMID:22782902]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:23625923]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0000321
re-entry into mitotic cell cycle after pheromone arrest
[PMID:12588993]"
/experiment="EXISTENCE:mutant phenotype:GO:0016239
positive regulation of macroautophagy [PMID:22782902]"
/experiment="EXISTENCE:mutant phenotype:GO:0031573 mitotic
intra-S DNA damage checkpoint signaling [PMID:22782902]"
/note="Protein involved in recovery from cell cycle
arrest; acts in response to pheromone; also involved in
regulation of intra-S DNA damage checkpoint and autophagy;
is essential for dephosphorylation of Atg13p; interacts
with Far3p, Far7p, Far8p, Far9p, Far10p and with the
phosphatases Pph21p, Pph22p and Pph3p; has similarity to
the N- and C-termini of N. crassa HAM-2; similar to human
Fam40A and Fam40B"
/codon_start=1
/product="Far11p"
/protein_id="NP_014272.3"
/db_xref="GeneID:855596"
/db_xref="SGD:S000005071"
/translation="MNASGRSHSKGPIIRSVSLEDLKRNSSFKGNLKYKDEVTSHKEP
QVGTLSNEELLKDLDNMLRGKLNMGRNSFHADKRNKSDGNISALTFKARSGLEGDIRT
IDIQQDSSDENDNFKFSDDGVNKDRNNEKDNNTDNAVEFQDDAEEAEEENEDESFANV
DELDGFDLNKVSDGKHVPINEKGEVDYNMPVDKEFQKSLDQCAASLEERSSAPYALQR
AVDWELKMFYSLEDELSEWFCSSDYMHFGQTQTLFKQKITQPQLFFDDENYAASVVEC
LIEDIPNSLASNLLALSYISMGCFAFTNSKSEHTKIIRRNNLMLVPHIQEIVHAFKKI
AISCRDDNRNLKKQTILLFHSSTILYFICSICIEGRGENPEAVNVVIDAFEKTDLLEF
LTKYIENWRWNSRLAMRIRNMISLLFKLIVLQFGDSSVYKQTKSSIYNLHGLTYPSKH
PEKLSISPLHYQAFREDITSRFPDYNMPSSGLPKDVDKSESLSQFLEIPRPKSKNPLN
MALIVPEKHIATPAPSPPSSPQLMHLGEGPRPRKSFQTNMAYPCLYPSDNEGSEDDTL
EDRIDLNIERKPDNDIVIPFSTEEAARILSESLEIKLSTKQLWYERDLFMITERGWKQ
QLENEPYDYAALNHDANSSKEEKSAICIMQRIDKYYKSCLSSFNSLVFVLLQTMESSL
TNNFHRKSEVSDKNLLNMLTPQLEIVRAKELSLKSAAGILHALLKWFKLSHILKFEHL
AVVIHDSRYINTCASILSKYSEVYPERVFNKYVQTPNSFWKECSLSNESYRESYSVDD
SGEVDTEIMPSFAYLLRILRKVTGNKTQRLKELPLSIGILFKRYYRLFNLDMYHPILK
ITRELTPFKNKRWKSEHMELISGVYLYEKLELTDNWVTGKDISGELSDACGQEIALRA
LLQFYNFQHYEISMEDLGYGHRNSSSQDLLNKESEYLNI"
gene <387227..>389767
/gene="SPC98"
/locus_tag="YNL126W"
/db_xref="GeneID:855597"
mRNA <387227..>389767
/gene="SPC98"
/locus_tag="YNL126W"
/product="Spc98p"
/transcript_id="NM_001182964.1"
/db_xref="GeneID:855597"
CDS 387227..389767
/gene="SPC98"
/locus_tag="YNL126W"
/experiment="EXISTENCE:direct assay:GO:0005822 inner
plaque of spindle pole body [PMID:9215630]"
/experiment="EXISTENCE:direct assay:GO:0005824 outer
plaque of spindle pole body [PMID:9215630]"
/experiment="EXISTENCE:direct assay:GO:0007020 microtubule
nucleation [PMID:24842996|PMID:11950928]"
/experiment="EXISTENCE:direct assay:GO:0008275
gamma-tubulin small complex
[PMID:9130700|PMID:25599398|PMID:9384578]"
/experiment="EXISTENCE:mutant phenotype:GO:0007052 mitotic
spindle organization [PMID:9153752]"
/experiment="EXISTENCE:mutant phenotype:GO:0043015
gamma-tubulin binding [PMID:8670895]"
/experiment="EXISTENCE:physical interaction:GO:0008275
gamma-tubulin small complex [PMID:9130700]"
/experiment="EXISTENCE:physical interaction:GO:0043015
gamma-tubulin binding [PMID:9130700|PMID:8670895]"
/note="Component of the microtubule-nucleating Tub4p
(gamma-tubulin) complex; interacts with Spc110p at the
spindle pole body (SPB) inner plaque and with Spc72p at
the SPB outer plaque"
/codon_start=1
/product="Spc98p"
/protein_id="NP_014273.1"
/db_xref="GeneID:855597"
/db_xref="SGD:S000005070"
/translation="MELEPTLFGIIEALAPQLLSQSHLQTFVSDVVNLLRSSTKSATQ
LGPLIDFYKLQSLDSPETTIMWHKIEKFLDALFGIQNTDDMVKYLSVFQSLLPSNYRA
KIVQKSSGLNMENLANHEHLLSPVRAPSIYTEASFENMDRFSERRSMVSSPNRYVPSS
TYSSVTLRQLSNPYYVNTIPEEDILKYVSYTLLATTSALFPFDHEQIQIPSKIPNFES
GLLHLIFEAGLLYQSLGYKVEKFRMLNISPMKKALIIEISEELQNYTAFVNNLVSSGT
VVSLKSLYREIYENIIRLRIYCRFTEHLEELSGDTFLIELNIFKSHGDLTIRKIATNL
FNSMISLYYEYLMNWLTKGLLRATYGEFFIAENTDTNGTDDDFIYHIPIEFNQERVPA
FIPKELAYKIFMIGKSYIFLEKYCKEVQWTNEFSKKYHVLYQSNSYRGISTNFFEIIN
DQYSEIVNHTNQILNQKFHYRDVVFALKNILLMGKSDFMDALIEKANDILATPSDSLP
NYKLTRVLQEAVQLSSLRHLMNSPRNSSVINGLDARVLDLGHGSVGWDVFTLDYILYP
PLSLVLNVNRPFGRKEYLRIFNFLWRFKKNNYFYQKEMLKSNDIIRSFKKIRGYNPLI
RDIINKLSRISILRTQFQQFNSKMESYYLNCIIEENFKEMTRKLQRTENKSQNQFDLI
RLNNGTIELNGILTPKAEVLTKSSSSKPQKHAIEKTLNIDELESVHNTFLTNILSHKL
FATNTSEISVGDYSGQPYPTSLVLLLNSVYEFVKVYCNLNDIGYEIFIKMNLNDHEAS
NGLLGKFNTNLKEIVSQYKNFKDRLYIFRADLKNDGDEELFLLSKSLR"
gene complement(<390146..>392167)
/gene="ESBP6"
/locus_tag="YNL125C"
/gene_synonym="MCH3"
/db_xref="GeneID:855598"
mRNA complement(<390146..>392167)
/gene="ESBP6"
/locus_tag="YNL125C"
/gene_synonym="MCH3"
/product="Esbp6p"
/transcript_id="NM_001182963.1"
/db_xref="GeneID:855598"
CDS complement(390146..392167)
/gene="ESBP6"
/locus_tag="YNL125C"
/gene_synonym="MCH3"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:11536335]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="Protein with similarity to monocarboxylate
permeases; appears not to be involved in transport of
monocarboxylates such as lactate, pyruvate or acetate
across the plasma membrane"
/codon_start=1
/product="Esbp6p"
/protein_id="NP_014274.1"
/db_xref="GeneID:855598"
/db_xref="SGD:S000005069"
/translation="MSTHSNDYFSASSGMVSETSSEVSSINSSQPVSFSKASIAAPVP
CSDLHSTKSNDASRKLSISRTLTNRLNDIKKAVDDDNLQTEENSADVNKILESRFDVA
DAIRLQHNESVQSKLNIPVTHTTTAGASLSAPSSSAFSASSIQNDTTEHKASMDSKLM
RNRLYPASTKHSGKDLEAQGITEFEPDEPTVKKVFTNKSTGQLELPPDGGYGWVVTFC
VFLTMFSTWGCNASFGVDLAYYLNHDTYPGASKYDYALIAGLTVFLGQLLSPLVMALM
RIIGLRTTMLFGDAVMLAAYLLASFTTKLWQLYVTQGFMVGCSISLIFVPATTVLPGW
FLKKRAVAMGVSLLGTGAGGVVYGLATNKMLSDFGNTRWCLRIIGISCSISVLVAIAL
LKERNPTPAIGLKSPRAMFEQLKAMFSLKVITKPFVVLIALWFMFALFAYNMMVFTLS
SYAISKGLSSHDASTLTAILNGSQSIGRPLMGLAGDKFGRANVTIVLTTLLTIYMFAF
WIPAHTFVQLIFFSILVGSCVGVANVMNTVLIADMVKPEEFLPAWAFVNYCGAPFLLV
CEVIAQALTVEKDKSNPYLHAQIFCGCCFIAALILISILREYSIRMKLTERQAMTNEK
LKEWKASEYDTDSADEDWGKLKERKTKYDLLLGPGIKKYFLRMVYPMKV"
gene <392892..>394370
/gene="NAF1"
/locus_tag="YNL124W"
/db_xref="GeneID:855599"
mRNA <392892..>394370
/gene="NAF1"
/locus_tag="YNL124W"
/product="RNA-binding snoRNP assembly protein"
/transcript_id="NM_001182962.3"
/db_xref="GeneID:855599"
CDS 392892..394370
/gene="NAF1"
/locus_tag="YNL124W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12515383]"
/experiment="EXISTENCE:direct assay:GO:0005654 nucleoplasm
[PMID:12242285|PMID:12515383]"
/experiment="EXISTENCE:mutant phenotype:GO:0000493 box
H/ACA snoRNP assembly
[PMID:12228251|PMID:12242285|PMID:12515383]"
/experiment="EXISTENCE:physical interaction:GO:0000493 box
H/ACA snoRNP assembly [PMID:12228251|PMID:12515383]"
/experiment="EXISTENCE:physical interaction:GO:0003723 RNA
binding [PMID:12515383]"
/note="RNA-binding protein required for the assembly of
box H/ACA snoRNPs; thus required for pre-rRNA processing;
forms a complex with Shq1p and interacts with H/ACA snoRNP
components Nhp2p and Cbf5p; similar to Gar1p"
/codon_start=1
/product="RNA-binding snoRNP assembly protein"
/protein_id="NP_014275.3"
/db_xref="GeneID:855599"
/db_xref="SGD:S000005068"
/translation="MSDDLFSKALENPDQDLNVELPKDDVDLGLLGDGGNERKTDEPV
ADAERSTGLGSGSSESESDSGSDSDSDSGSSGSEDDSADQDVEGEDEGGDAIENEDED
EDPSPSGPILSKNEILEETVPELPEDYEISEKTIITPIGVLKSAFENNIIIHATMSGE
KRVLKEGSIFCLEDRTLIGMLTEVFGPLQNPFYRIKLPDSKKNLFDELKVRLGEKAFI
VTPDAHWIDTFELKRNKGTDASNGYDEELPEEEQEFSDDEKEALFKKMKKQQRQRKKR
DNRKLANDSDNVKVKRARQPKANSLPKLVPPLGMSSNAPMQHGYKSRNARENIKRESS
ATSNRNGSSPVPITQHHQQQFSANNYPFPQQPNGMPYPPYSPFPQPTNFQYPPPPFGQ
ATPAQFSNTVPYGSLPPAYNNMSPPTQQSFMPMTQSQPPLPYGVPPMNQMQNPMYIQP
PPQAPPQGNGNFQQVMELHQILLQQQQQQHQYQHQHQQDPRT"
gene <394685..>397678
/gene="NMA111"
/locus_tag="YNL123W"
/gene_synonym="YNM3"
/db_xref="GeneID:855600"
mRNA <394685..>397678
/gene="NMA111"
/locus_tag="YNL123W"
/gene_synonym="YNM3"
/product="Nma111p"
/transcript_id="NM_001182961.1"
/db_xref="GeneID:855600"
CDS 394685..397678
/gene="NMA111"
/locus_tag="YNL123W"
/gene_synonym="YNM3"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095|PMID:14657274]"
/experiment="EXISTENCE:direct assay:GO:0008236 serine-type
peptidase activity [PMID:18946088]"
/experiment="EXISTENCE:mutant phenotype:GO:0004252
serine-type endopeptidase activity [PMID:29861160]"
/experiment="EXISTENCE:mutant phenotype:GO:0006629 lipid
metabolic process [PMID:16470384]"
/experiment="EXISTENCE:mutant phenotype:GO:0006915
apoptotic process
[PMID:14657274|PMID:16470384|PMID:16608876]"
/experiment="EXISTENCE:mutant phenotype:GO:0030163 protein
catabolic process [PMID:18946088]"
/experiment="EXISTENCE:mutant phenotype:GO:0033554
cellular response to stress [PMID:29861160]"
/experiment="EXISTENCE:mutant phenotype:GO:0034605
cellular response to heat [PMID:18946088]"
/experiment="EXISTENCE:mutant phenotype:GO:0120174
stress-induced homeostatically regulated protein
degradation pathway [PMID:29861160]"
/experiment="EXISTENCE:physical interaction:GO:0006629
lipid metabolic process [PMID:16470384]"
/note="Serine protease and general molecular chaperone;
cleaves Roq1p, which modifies the substrate specificity of
the Ubr1p Ub-ligase, promoting the stress-induced
homeostatically-regulated protein degradation (SHRED) of
misfolded and native ER-membrane and cytosolic proteins;
chaperone activity involved in the heat stress response;
promotes apoptosis through proteolysis of Bir1p; role in
lipid homeostasis; mammalian Omi/HtrA2 serine protease
family member"
/codon_start=1
/product="Nma111p"
/protein_id="NP_014276.1"
/db_xref="GeneID:855600"
/db_xref="SGD:S000005067"
/translation="MTISLSNIKKRDHSKISDGTSGESSLVKRKQLESATGDQEEEYT
DHEIIIEPLHFANNNNTVLTDSENYLRWQNTISNVVKSVVSIHFSQVAPFDCDSALVS
EATGFVVDAKLGIILTNRHVVGPGPFVGYVVFDNHEECDVIPIYRDPVHDFGFLKFDP
KNIKYSKIKALTLKPSLAKVGSEIRVVGNDAGEKLSILAGFISRIDRNAPEYGELTYN
DFNTEYIQAAASASGGSSGSPVVNIDGYAVALQAGGSTEASTDFFLPLDRILRALICI
QTNKPITRGTIQVQWLLKPYDECRRLGLTSERESEARAKFPENIGLLVAETVLREGPG
YDKIKEGDTLISINGETISSFMQVDKIQDENVGKEIQLVIQRGGVECTVTCTVGDLHA
ITPHRYVEVCGATFHELSYQMARFYALPVRGVFLSSASGSFNFDSKERVGWIVDSIDN
KETPDLDTFIEIMKTIPDRKRVTVRYHHLTDQHSPLVTSIYIDRHWCNEFRVYTRNDT
TGIWDYKNVADPLPADALKPRSAKIIPIPVNNEKVAKLSSSLCTVATMAAVPLDSLSA
DILKTSGLIIDAEKGYVLVSRRVVPHDCLDTFVTIADSLVVPATVEFLHPTHNFAIVK
YDPELVKAPLITPKLSTTRMKRGDKLQFIGFTQNDRIVTSETTVTDISSVSIPSNLIP
RYRATNLEAISIDCNVSTRCNSGILTDNDGTVRGLWLPFLGERLENKEKVYLMGLDIM
DCREVIDILKNGGKPRVSIVDAGFGSISVLQARIRGVPEEWIMRMEHESNNRLQFITV
SRVSYTEDKIHLETGDVILSVNGKLVTEMNDLNGVVSSADGILPSAMLDFKVVRDGNI
VDLKIKTVEVQETDRFVIFAGSILQKPHHAVLQAMVDVPKGVYCTFRGESSPALQYGI
SATNFITHVNEIETPDLDTFLKVVKTIPDNSYCKMRLMTFDNVPFAISLKTNYHYFPT
AELKRDNITHKWIEKEFTGNSQSEK"
gene complement(<398023..>398370)
/gene="MRP35"
/locus_tag="YNL122C"
/gene_synonym="bL35m"
/db_xref="GeneID:855601"
mRNA complement(<398023..>398370)
/gene="MRP35"
/locus_tag="YNL122C"
/gene_synonym="bL35m"
/product="mitochondrial 54S ribosomal protein bL35m MRP35"
/transcript_id="NM_001182960.1"
/db_xref="GeneID:855601"
CDS complement(398023..398370)
/gene="MRP35"
/locus_tag="YNL122C"
/gene_synonym="bL35m"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:24675956]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:24675956]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095|PMID:17054397]"
/experiment="EXISTENCE:direct assay:GO:0005762
mitochondrial large ribosomal subunit [PMID:24675956]"
/note="Mitochondrial ribosomal protein of the large
subunit; homologous to bacterial L35 and human MRPL35
ribosomal proteins"
/codon_start=1
/product="mitochondrial 54S ribosomal protein bL35m MRP35"
/protein_id="NP_014277.1"
/db_xref="GeneID:855601"
/db_xref="SGD:S000005066"
/translation="MKISLHNKRQRGDQNQNMSVFNVLKPLLKGSNSFKVKLNGFLFN
NVSTITIRTLMKTHKGTAKRWRRTGNTFKRGIAGRKHGNIGWSHRSLKALTGRKIAHP
AYSKHLKRLLPYH"
gene complement(<398684..>400537)
/gene="TOM70"
/locus_tag="YNL121C"
/gene_synonym="MAS70; MOM72; OMP1"
/db_xref="GeneID:855602"
mRNA complement(<398684..>400537)
/gene="TOM70"
/locus_tag="YNL121C"
/gene_synonym="MAS70; MOM72; OMP1"
/product="protein channel TOM70"
/transcript_id="NM_001182959.3"
/db_xref="GeneID:855602"
CDS complement(398684..400537)
/gene="TOM70"
/locus_tag="YNL121C"
/gene_synonym="MAS70; MOM72; OMP1"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961|PMID:11502169]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane
[PMID:16407407|PMID:16689936]"
/experiment="EXISTENCE:direct assay:GO:0005742
mitochondrial outer membrane translocase complex
[PMID:9774667]"
/experiment="EXISTENCE:direct assay:GO:0044233
mitochondria-associated endoplasmic reticulum membrane
contact site [PMID:37366411]"
/experiment="EXISTENCE:direct assay:GO:0045039 protein
insertion into mitochondrial inner membrane
[PMID:2177474]"
/experiment="EXISTENCE:genetic interaction:GO:0006626
protein targeting to mitochondrion [PMID:18007655]"
/experiment="EXISTENCE:mutant phenotype:GO:0008320 protein
transmembrane transporter activity [PMID:2177474]"
/experiment="EXISTENCE:mutant phenotype:GO:0030150 protein
import into mitochondrial matrix [PMID:8132642]"
/experiment="EXISTENCE:mutant phenotype:GO:0030943
mitochondrion targeting sequence binding [PMID:11054285]"
/experiment="EXISTENCE:mutant phenotype:GO:0045039 protein
insertion into mitochondrial inner membrane
[PMID:2177474]"
/note="Component of the TOM (translocase of outer
membrane) complex; involved in recognition and initial
import steps for all mitochondrially-directed proteins,
acting as a receptor for incoming precursors; role in the
distribution of tail-anchored proteins between
mitochondria and peroxisomes; moonlighting role as a
transcriptional regulator of mitochondrial proteins;
age-dependent reduction in Tom70p results in mitochondrial
dysfunction, a hallmark of aging; localizes to
mitochondria-ER contact sites"
/codon_start=1
/product="protein channel TOM70"
/protein_id="NP_014278.3"
/db_xref="GeneID:855602"
/db_xref="SGD:S000005065"
/translation="MKSFITRNKTAILATVAATGTAIGAYYYYNQLQQQQQRGKKNTI
NKDEKKDTKDSQKETEGAKKSTAPSNPPIYPVSSNGEPDFSNKANFTAEEKDKYALAL
KDKGNQFFRNKKYDDAIKYYNWALELKEDPVFYSNLSACYVSVGDLKKVVEMSTKALE
LKPDYSKVLLRRASANEGLGKFADAMFDLSVLSLNGDFNDASIEPMLERNLNKQAMSK
LKEKFGDIDTATATPTELSTQPAKERKDKQENLPSVTSMASFFGIFKPELTFANYDES
NEADKELMNGLSNLYKRSPESYDKADESFTKAARLFEEQLDKNNEDEKLKEKLAISLE
HTGIFKFLKNDPLGAHEDIKKAIELFPRVNSYIYMALIMADRNDSTEYYNYFDKALKL
DSNNSSVYYHRGQMNFILQNYDQAGKDFDKAKELDPENIFPYIQLACLAYRENKFDDC
ETLFSEAKRKFPEAPEVPNFFAEILTDKNDFDKALKQYDLAIELENKLDGIYVGIAPL
VGKATLLTRNPTVENFIEATNLLEKASKLDPRSEQAKIGLAQMKLQQEDIDEAITLFE
ESADLARTMEEKLQAITFAEAAKVQQRIRSDPVLAKKIQETLAKLREQGLM"
gene <401040..>402521
/gene="NCS2"
/locus_tag="YNL119W"
/gene_synonym="TUC2"
/db_xref="GeneID:855603"
mRNA <401040..>402521
/gene="NCS2"
/locus_tag="YNL119W"
/gene_synonym="TUC2"
/product="Ncs2p"
/transcript_id="NM_001182957.3"
/db_xref="GeneID:855603"
CDS 401040..402521
/gene="NCS2"
/locus_tag="YNL119W"
/gene_synonym="TUC2"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0001403
invasive growth in response to glucose limitation
[PMID:14551258]"
/experiment="EXISTENCE:mutant phenotype:GO:0002143 tRNA
wobble position uridine thiolation
[PMID:19151091|PMID:18664566|PMID:19145231|PMID:17018299|P
MID:18755837]"
/experiment="EXISTENCE:mutant phenotype:GO:0007124
pseudohyphal growth [PMID:14551258]"
/experiment="EXISTENCE:mutant phenotype:GO:0032447 protein
urmylation [PMID:14551258]"
/note="Protein required for uridine thiolation of Lys(UUU)
and Glu(UUC) tRNAs; required for the thiolation of uridine
at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has
a role in urmylation and in invasive and pseudohyphal
growth; inhibits replication of Brome mosaic virus in S.
cerevisiae"
/codon_start=1
/product="Ncs2p"
/protein_id="NP_014280.3"
/db_xref="GeneID:855603"
/db_xref="SGD:S000005063"
/translation="MECQRCPASARNPATVESRKEKFCDECFIKFVSTKQRKQMMKDE
YFRNLFKVIYPFEKEGSVSKILLPLSHSDSGSLVMLDIVHDLLLEQTKQHNNRTGFTV
DVLTVFTEENVSVIKERMESLINEKMSQLNKISNIFNVHFIDVNEFFNNASEVSTFII
DNENFEIFSKSKSVDDSNILTLKEILGKYCLNNSSRSDLISIIKTQLIKHFAYENGYN
AIMWGHSMTKLSEVIISLVVKGKGSQIATFLDSESFDTLNNKPCKYKNLYPMKDLLSV
EIESFLQIRNLAQFLINVEETNVKPNCLIARKSLPSLGQQKLVKNMTINEITNKYFQD
IQNDYSNIISTVLRTADKLTQPKSSMAKPSQCQICQSKIYTNPSNWLNRITVTSPYPV
ETTEEKYLFKQWQDSKLGQSHTHYVELLNEIKQGASNSLDVEDGDVKLCYGCLILLNT
SIKDKNLVWPKVDTMDITANATNKNKELSQILDQFEINSDGEE"
gene complement(<402652..>405564)
/gene="DCP2"
/locus_tag="YNL118C"
/gene_synonym="PSU1"
/db_xref="GeneID:855605"
mRNA complement(<402652..>405564)
/gene="DCP2"
/locus_tag="YNL118C"
/gene_synonym="PSU1"
/product="decapping enzyme complex catalytic subunit"
/transcript_id="NM_001182956.1"
/db_xref="GeneID:855605"
CDS complement(402652..405564)
/gene="DCP2"
/locus_tag="YNL118C"
/gene_synonym="PSU1"
/EC_number="3.6.1.62"
/experiment="EXISTENCE:direct assay:GO:0000290
deadenylation-dependent decapping of nuclear-transcribed
mRNA [PMID:10508173|PMID:11139489]"
/experiment="EXISTENCE:direct assay:GO:0000932 P-body
[PMID:23509072|PMID:12730603]"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:23706738]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:11139489]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:23706738|PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:23706738|PMID:11914276|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0016787 hydrolase
activity [PMID:12486012]"
/experiment="EXISTENCE:direct assay:GO:0098562 cytoplasmic
side of membrane [PMID:27146487]"
/experiment="EXISTENCE:direct assay:GO:0098745 RNA
decapping complex [PMID:12554866]"
/experiment="EXISTENCE:genetic interaction:GO:0034063
stress granule assembly [PMID:20513766]"
/experiment="EXISTENCE:mutant phenotype:GO:0000290
deadenylation-dependent decapping of nuclear-transcribed
mRNA [PMID:10508173]"
/experiment="EXISTENCE:mutant phenotype:GO:0031087
deadenylation-independent decapping of nuclear-transcribed
mRNA [PMID:15225544]"
/experiment="EXISTENCE:mutant phenotype:GO:0034063 stress
granule assembly [PMID:20513766]"
/experiment="EXISTENCE:mutant phenotype:GO:0060261
positive regulation of transcription initiation by RNA
polymerase II [PMID:23706738]"
/experiment="EXISTENCE:physical interaction:GO:0098745 RNA
decapping complex [PMID:10508173]"
/note="Catalytic subunit of Dcp1p-Dcp2p decapping enzyme
complex; removes 5' cap structure from mRNAs prior to
their degradation; also enters nucleus and positively
regulates transcription initiation; nudix hydrolase family
member; forms cytoplasmic foci upon DNA replication
stress; human homolog DCP2 complements yeast dcp2
thermosensitive mutant"
/codon_start=1
/product="decapping enzyme complex catalytic subunit"
/protein_id="NP_014281.1"
/db_xref="GeneID:855605"
/db_xref="SGD:S000005062"
/translation="MSLPLRHALENVTSVDRILEDLLVRFIINCPNEDLSSVERELFH
FEEASWFYTDFIKLMNPTLPSLKIKSFAQLIIKLCPLVWKWDIRVDEALQQFSKYKKS
IPVRGAAIFNENLSKILLVQGTESDSWSFPRGKISKDENDIDCCIREVKEEIGFDLTD
YIDDNQFIERNIQGKNYKIFLISGVSEVFNFKPQVRNEIDKIEWFDFKKISKTMYKSN
IKYYLINSMMRPLSMWLRHQRQIKNEDQLKSYAEEQLKLLLGITKEEQIDPGRELLNM
LHTAVQANSNNNAVSNGQVPSSQELQHLKEQSGEHNQQKDQQSSFSSQQQPSIFPSLS
EPFANNKNVIPPTMPMANVFMSNPQLFATMNGQPFAPFPFMLPLTNNSNSANPIPTPV
PPNFNAPPNPMAFGVPNMHNLSGPAVSQPFSLPPAPLPRDSGYSSSSPGQLLDILNSK
KPDSNVQSSKKPKLKILQRGTDLNSIKQNNNDETAHSNSQALLDLLKKPTSSQKIHAS
KPDTSFLPNDSVSGIQDAEYEDFESSSDEEVETARDERNSLNVDIGVNVMPSEKDSRR
SQKEKPRNDASKTNLNASAESNSVEWGPGKSSPSTQSKQNSSVGMQNKYRQEIHIGDS
DAYEVFESSSDEEDGKKLEELEQTQDNSKLISQDILKENNFQDGEVPHRDMPTESNKS
INETVGLSSTTNTVKKVPKVKILKRGETFASLANDKKAFDSSSNVSSSKDLLQMLRNP
ISSTVSSNQQSPKSQHLSGDEEIMMMLKRNSVSKPQNSEENASTSSINDANASELLGM
LKQKEKDITAPKQPYNVDSYSQKNSAKGLLNILKKNDSTGYPRTEGGPSSEMSTSMKR
NDATNNQELDKNSTELLNYLKPKPLNDGYENISNKDSSHELLNILHGNKNSSAFNNNV
YATDGYSLASDNNENSSNKLLNMLQNRSSAINEPNFDVRSNGTSGSNELLSILHRK"
gene <406358..>408022
/gene="MLS1"
/locus_tag="YNL117W"
/db_xref="GeneID:855606"
mRNA <406358..>408022
/gene="MLS1"
/locus_tag="YNL117W"
/product="malate synthase MLS1"
/transcript_id="NM_001182955.1"
/db_xref="GeneID:855606"
CDS 406358..408022
/gene="MLS1"
/locus_tag="YNL117W"
/EC_number="2.3.3.9"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:27678487|PMID:2211514]"
/experiment="EXISTENCE:direct assay:GO:0005782 peroxisomal
matrix [PMID:11846793]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762|PMID:11846793]"
/experiment="EXISTENCE:mutant phenotype:GO:0004474 malate
synthase activity [PMID:23642236|PMID:8878673]"
/experiment="EXISTENCE:mutant phenotype:GO:0006097
glyoxylate cycle [PMID:8878673|PMID:23642236]"
/note="Malate synthase, enzyme of the glyoxylate cycle;
involved in utilization of non-fermentable carbon sources;
expression is subject to carbon catabolite repression;
localizes in peroxisomes during growth on oleic acid,
otherwise cytosolic; can accept butyryl-CoA as acyl-CoA
donor in addition to traditional substrate acetyl-CoA"
/codon_start=1
/product="malate synthase MLS1"
/protein_id="NP_014282.1"
/db_xref="GeneID:855606"
/db_xref="SGD:S000005061"
/translation="MVKVSLDNVKLLVDVDKEPFFKPSSTTVGDILTKDALEFIVLLH
RTFNNKRKQLLENRQVVQKKLDSGSYHLDFLPETANIRNDPTWQGPILAPGLINRSTE
ITGPPLRNMLINALNAPVNTYMTDFEDSASPTWNNMVYGQVNLYDAIRNQIDFDTPRK
SYKLNGNVANLPTIIVRPRGWHMVEKHLYVDDEPISASIFDFGLYFYHNAKELIKLGK
GPYFYLPKMEHHLEAKLWNDVFCVAQDYIGIPRGTIRATVLIETLPAAFQMEEIIYQL
RQHSSGLNCGRWDYIFSTIKRLRNDPNHILPNRNQVTMTSPFMDAYVKRLINTCHRRG
VHAMGGMAAQIPIKDDPAANEKAMTKVRNDKIRELTNGHDGSWVAHPALAPICNEVFI
NMGTPNQIYFIPENVVTAANLLETKIPNGEITTEGIVQNLDIGLQYMEAWLRGSGCVP
INNLMEDAATAEVSRCQLYQWVKHGVTLKDTGEKVTPELTEKILKEQVERLSKASPLG
DKNKFALAAKYFLPEIRGEKFSEFLTTLLYDEIVSTKATPTDLSKL"
gene <408341..>409909
/gene="DMA2"
/locus_tag="YNL116W"
/gene_synonym="CHF2"
/db_xref="GeneID:855607"
mRNA <408341..>409909
/gene="DMA2"
/locus_tag="YNL116W"
/gene_synonym="CHF2"
/product="ubiquitin-conjugating protein DMA2"
/transcript_id="NM_001182954.3"
/db_xref="GeneID:855607"
CDS 408341..409909
/gene="DMA2"
/locus_tag="YNL116W"
/gene_synonym="CHF2"
/EC_number="2.3.2.27"
/experiment="EXISTENCE:direct assay:GO:0004842
ubiquitin-protein transferase activity [PMID:18202552]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0051865 protein
autoubiquitination [PMID:18202552]"
/experiment="EXISTENCE:genetic interaction:GO:0000132
establishment of mitotic spindle orientation
[PMID:15146058|PMID:22570619]"
/experiment="EXISTENCE:genetic interaction:GO:0000921
septin ring assembly [PMID:22570619|PMID:15146058]"
/experiment="EXISTENCE:genetic interaction:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:21562220]"
/experiment="EXISTENCE:genetic interaction:GO:0031578
mitotic spindle orientation checkpoint signaling
[PMID:22570619|PMID:15146058]"
/experiment="EXISTENCE:genetic interaction:GO:0032186
cellular bud neck septin ring organization
[PMID:22570619]"
/experiment="EXISTENCE:genetic interaction:GO:0097271
protein localization to bud neck [PMID:22570619]"
/experiment="EXISTENCE:mutant phenotype:GO:0004842
ubiquitin-protein transferase activity [PMID:18202552]"
/experiment="EXISTENCE:mutant phenotype:GO:0005934
cellular bud tip [PMID:23442799]"
/experiment="EXISTENCE:mutant phenotype:GO:0032177
cellular bud neck split septin rings [PMID:23442799]"
/experiment="EXISTENCE:mutant phenotype:GO:0090337
regulation of formin-nucleated actin cable assembly
[PMID:27449057]"
/experiment="EXISTENCE:physical interaction:GO:0090337
regulation of formin-nucleated actin cable assembly
[PMID:27449057]"
/note="Ubiquitin-protein ligase (E3); controls septin
dynamics and spindle position checkpoint (SPOC) with
ligase Dma1p by regulating recruitment of Elm1p to bud
neck; regulates levels of eIF2 subunit Gcd11p, as well as
abundance, localization, and ubiquitination of Cdk
inhibitory kinase Swe1p; ortholog of human RNF8, similar
to human Chfr; contains FHA and RING finger domains; DMA2
has a paralog, DMA1, that arose from the whole genome
duplication"
/codon_start=1
/product="ubiquitin-conjugating protein DMA2"
/protein_id="NP_014283.3"
/db_xref="GeneID:855607"
/db_xref="SGD:S000005060"
/translation="MYTPIPANTPAPTAPTSSMTSNSSSASNANTTSSSGINPRNRAS
GTPSNERARPASGISSFLNTFGIRQNSQTASSSAAPDQRLFGTTPSNSHMSVAMESID
TAPQQQEPRLHHPIQMPLSAQFHVHRNYQLPISISLTAPTTTDHQQSSAHNFEGNNVG
NVQESLNQRQPNGTNNTTTSIISMAPAATTRNIVGGADGSTIVNNSQEMYKNLRHLIY
AANQPNGTEILHLDLPATSAEESNNMFNVDEVTLKQRKDKHGLFSIRLTPFIDSSSTT
NQGLFFEPIIRKAGPGSQLVIGRYTERVRDAISKIPEQYHPVVFKSKVVSRTHGCFKV
DSQGNWYIKDVKSSSGTFLNHQRLSPASSLSKDTPLRDGDILQLGMDFRGGTEEIYRC
VRMRIELNRSWKLKANSFNKEALQRLQNLQKLTTGIEEEDCSICLCKIKPCQAIFISP
CAHSWHFRCVRRLVMLSYPQFVCPNCRSSCDLEASFESSDEEDESDVESEGDQLVDQL
SVLMETSKDVDSHP"
gene complement(<410119..>412053)
/locus_tag="YNL115C"
/db_xref="GeneID:855608"
mRNA complement(<410119..>412053)
/locus_tag="YNL115C"
/product="uncharacterized protein"
/transcript_id="NM_001182953.1"
/db_xref="GeneID:855608"
CDS complement(410119..412053)
/locus_tag="YNL115C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to mitochondria; YNL115C is
not an essential gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014284.1"
/db_xref="GeneID:855608"
/db_xref="SGD:S000005059"
/translation="MKANGLDNDPARTGMERTDIDSEHPEAQPLLNNNHRTLGAGSAN
GPAVNEGRDIESDGFIKDSLFQIRKGYRIFIHNSKWILNILILINTIWLVTTLISDFF
FNINILFGFSNRYASFNDLTLIFISIIANSFNLWFNKLGLYSALDYSLNVTLCVLTLF
NLALTYLIKYTRQRIGFVGTFTYLWTSFSFFIGAILDWYLLFYNNSINEPLEERRIDD
ANISTFNENHTNSTENRDRSQYGSGSPTPTHRSQLVQNKHTLTEWVSIGFRNTIKFLI
LIFFALFTLNTLLTTLDTYRLTHKLPITVQSPSYEAFHYVDAAKTYQLHITCYGDVFD
QENNTDLSENKKQPIILFEHGGYDTGYLSATWIEELYHLDKIQRYCLYDRPGYGLSDS
PPAPISIAMVAESLRYALIKDAKIKGPFTTVGYDLGGLFTRVFTAKNVDIVDSMMLVE
SWHEELLLKNYIQRLLPPGRGDGDDGDDGNGNDGDGRNHDKTWLPSEIERHNEFRLWW
KGIWSSLGWRLQTSWLLAHHGSKERIYGRDMKYQGRFLRSKFLESVTSSILSYRDVTN
NAESLQNVKTSIVSSKEMVKKSALWGDWQRDLTKISHKTQEWKIVEGGHEIYKYGLGK
QQTQEVLLRLIGELGKLTED"
rep_origin 412267..412496
/note="ARS1417; Autonomously Replicating Sequence"
/db_xref="SGD:S000118386"
gene <412771..>413199
/gene="RPC19"
/locus_tag="YNL113W"
/db_xref="GeneID:855609"
mRNA <412771..>413199
/gene="RPC19"
/locus_tag="YNL113W"
/product="DNA-directed RNA polymerase core subunit RPC19"
/transcript_id="NM_001182951.1"
/db_xref="GeneID:855609"
CDS 412771..413199
/gene="RPC19"
/locus_tag="YNL113W"
/experiment="EXISTENCE:direct assay:GO:0003899
DNA-directed RNA polymerase activity
[PMID:3905793|PMID:9837969]"
/experiment="EXISTENCE:direct assay:GO:0005666 RNA
polymerase III complex [PMID:3905793|PMID:10611227]"
/experiment="EXISTENCE:direct assay:GO:0005736 RNA
polymerase I complex [PMID:11486042|PMID:9837969]"
/experiment="EXISTENCE:direct assay:GO:0006360
transcription by RNA polymerase I [PMID:9837969]"
/experiment="EXISTENCE:direct assay:GO:0042797 tRNA
transcription by RNA polymerase III [PMID:3905793]"
/note="RNA polymerase subunit AC19; common to RNA
polymerases I and III"
/codon_start=1
/product="DNA-directed RNA polymerase core subunit RPC19"
/protein_id="NP_014286.1"
/db_xref="GeneID:855609"
/db_xref="SGD:S000005057"
/translation="MTEDIEQKKTATEVTPQEPKHIQEEEEQDVDMTGDEEQEEEPDR
EKIKLLTQATSEDGTSASFQIVEEDHTLGNALRYVIMKNPDVEFCGYSIPHPSENLLN
IRIQTYGETTAVDALQKGLKDLMDLCDVVESKFTEKIKSM"
gene <413639..>416281
/gene="DBP2"
/locus_tag="YNL112W"
/db_xref="GeneID:855611"
mRNA join(<413639..414911,415914..>416281)
/gene="DBP2"
/locus_tag="YNL112W"
/product="DEAD-box ATP-dependent RNA helicase DBP2"
/transcript_id="NM_001182950.3"
/db_xref="GeneID:855611"
CDS join(413639..414911,415914..416281)
/gene="DBP2"
/locus_tag="YNL112W"
/EC_number="3.6.4.13"
/experiment="EXISTENCE:direct assay:GO:0002151
G-quadruplex RNA binding [PMID:30855040]"
/experiment="EXISTENCE:direct assay:GO:0003724 RNA
helicase activity [PMID:23721653]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:30902808]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22679025|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0006369 termination
of RNA polymerase II transcription [PMID:30902808]"
/experiment="EXISTENCE:direct assay:GO:0008186
ATP-dependent activity, acting on RNA [PMID:22679025]"
/experiment="EXISTENCE:direct assay:GO:0030515 snoRNA
binding [PMID:30902808]"
/experiment="EXISTENCE:direct assay:GO:0051880
G-quadruplex DNA binding [PMID:30855040]"
/experiment="EXISTENCE:genetic interaction:GO:0071042
nuclear polyadenylation-dependent mRNA catabolic process
[PMID:22679025]"
/experiment="EXISTENCE:mutant phenotype:GO:0006364 rRNA
processing [PMID:11585918]"
/experiment="EXISTENCE:mutant phenotype:GO:0008186
ATP-dependent activity, acting on RNA [PMID:22679025]"
/experiment="EXISTENCE:mutant phenotype:GO:0071042 nuclear
polyadenylation-dependent mRNA catabolic process
[PMID:22679025]"
/experiment="EXISTENCE:physical interaction:GO:0000184
nuclear-transcribed mRNA catabolic process,
nonsense-mediated decay [PMID:7883168]"
/note="ATP-dependent RNA helicase of the DEAD-box protein
family; strong preference for dsRNA; remodels RNP
structure for efficient transcription termination;
required for assembly of Yra1p, Nab2p and Mex67p onto mRNA
and formation of nuclear mRNP; involved in mRNA decay and
rRNA processing; may suppress transcription from cryptic
initiation sites; promotes Mex67p-mediated nuclear export
of mRNA by facilitating annealing with antisense RNAs to
create dsRNAs; binds G-quadruplex (G4) nucleic acids"
/codon_start=1
/product="DEAD-box ATP-dependent RNA helicase DBP2"
/protein_id="NP_014287.3"
/db_xref="GeneID:855611"
/db_xref="SGD:S000005056"
/translation="MTYGGRDQQYNKTNYKSRGGDFRGGRNSDRNSYNDRPQGGNYRG
GFGGRSNYNQPQELIKPNWDEELPKLPTFEKNFYVEHESVRDRSDSEIAQFRKENEMT
ISGHDIPKPITTFDEAGFPDYVLNEVKAEGFDKPTGIQCQGWPMALSGRDMVGIAATG
SGKTLSYCLPGIVHINAQPLLAPGDGPIVLVLAPTRELAVQIQTECSKFGHSSRIRNT
CVYGGVPKSQQIRDLSRGSEIVIATPGRLIDMLEIGKTNLKRVTYLVLDEADRMLDMG
FEPQIRKIVDQIRPDRQTLMWSATWPKEVKQLAADYLNDPIQVQVGSLELSASHNITQ
IVEVVSDFEKRDRLNKYLETASQDNEYKTLIFASTKRMCDDITKYLREDGWPALAIHG
DKDQRERDWVLQEFRNGRSPIMVATDVAARGIDVKGINYVINYDMPGNIEDYVHRIGR
TGRAGATGTAISFFTEQNKGLGAKLISIMREANQNIPPELLKYDRRSYGGGHPRYGGG
RGGRGGYGRRGGYGGGRGGYGGNRQRDGGWGNRGRSNY"
gene complement(<416940..>417302)
/gene="CYB5"
/locus_tag="YNL111C"
/db_xref="GeneID:855612"
mRNA complement(<416940..>417302)
/gene="CYB5"
/locus_tag="YNL111C"
/product="Cyb5p"
/transcript_id="NM_001182949.3"
/db_xref="GeneID:855612"
CDS complement(416940..417302)
/gene="CYB5"
/locus_tag="YNL111C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:12514182]"
/experiment="EXISTENCE:direct assay:GO:0009055 electron
transfer activity [PMID:10622712]"
/experiment="EXISTENCE:direct assay:GO:0016126 sterol
biosynthetic process [PMID:10622712]"
/note="Cytochrome b5; involved in the sterol and lipid
biosynthesis pathways; acts as an electron donor to
support sterol C5-6 desaturation"
/codon_start=1
/product="Cyb5p"
/protein_id="NP_014288.3"
/db_xref="GeneID:855612"
/db_xref="SGD:S000005055"
/translation="MPKVYSYQEVAEHNGPENFWIIIDDKVYDVSQFKDEHPGGDEII
MDLGGQDATESFVDIGHSDEALRLLKGLYIGDVDKTSERVSVEKVSTSENQSKGSGTL
VVILAILMLGVAYYLLNE"
gene complement(<417826..>418488)
/gene="NOP15"
/locus_tag="YNL110C"
/db_xref="GeneID:855613"
mRNA complement(<417826..>418488)
/gene="NOP15"
/locus_tag="YNL110C"
/product="rRNA-binding ribosome biosynthesis protein
NOP15"
/transcript_id="NM_001182948.1"
/db_xref="GeneID:855613"
CDS complement(417826..418488)
/gene="NOP15"
/locus_tag="YNL110C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10953080]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:11583614]"
/experiment="EXISTENCE:direct assay:GO:0006364 rRNA
processing [PMID:27643814]"
/experiment="EXISTENCE:direct assay:GO:0019843 rRNA
binding [PMID:27643814]"
/experiment="EXISTENCE:direct assay:GO:0030687
preribosome, large subunit precursor
[PMID:11583614|PMID:23212245|PMID:17443350]"
/experiment="EXISTENCE:direct assay:GO:0042273 ribosomal
large subunit biogenesis [PMID:27643814]"
/experiment="EXISTENCE:direct assay:GO:0043023 ribosomal
large subunit binding [PMID:27643814]"
/note="Constituent of 66S pre-ribosomal particles;
involved in 60S ribosomal subunit biogenesis; localizes to
both nucleolus and cytoplasm"
/codon_start=1
/product="rRNA-binding ribosome biosynthesis protein
NOP15"
/protein_id="NP_014289.1"
/db_xref="GeneID:855613"
/db_xref="SGD:S000005054"
/translation="MVKSTSKTSTKETVTKQPTEEKPIQEKEELALETSSSSSDEEDE
KDEDEIEGLAASDDEQSGTHKIKRLNPKKQANEKKSKDKKTLEEYSGIIYVSRLPHGF
HEKELSKYFAQFGDLKEVRLARNKKTGNSRHYGFLEFVNKEDAMIAQESMNNYLLMGH
LLQVRVLPKGAKIEKLYKYKKRVLVEKGITKPVKQLKDNMKQKHEERIKKLAKSGIEF
KW"
gene complement(<419014..>419826)
/locus_tag="YNL108C"
/gene_synonym="HUF"
/db_xref="GeneID:855615"
mRNA complement(<419014..>419826)
/locus_tag="YNL108C"
/gene_synonym="HUF"
/product="uncharacterized protein"
/transcript_id="NM_001182946.1"
/db_xref="GeneID:855615"
CDS complement(419014..419826)
/locus_tag="YNL108C"
/gene_synonym="HUF"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0016311
dephosphorylation [PMID:23569204]"
/experiment="EXISTENCE:direct assay:GO:0016791 phosphatase
activity [PMID:23569204]"
/note="Protein phosphatase; similar to prokaryotic
phosphotransfer enzymes; null mutant shows alterations in
glucose metabolism; GFP-fusion protein localizes to the
cytoplasm and nucleus; YNL108C has a paralog, TFC7, that
arose from the whole genome duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014291.1"
/db_xref="GeneID:855615"
/db_xref="SGD:S000005052"
/translation="MAIENIYIARHGYRSNWLPKGPYPPPPTGIDNDVPLSEHGVEQA
HELANYISKLDVKPEMIFSSPFYRCLETSKPTVEALKIPLYVDRGVGEWYKPDRPIIP
EPATHEVMSKFFPSMISPDWEPSIIPSNKGETEEDIFERCHKFWPVFIDRVERKFPNV
KTIMIVTHAATKSALGMNLLKFSSAKEPIDNKGTFIRNGSCAIDKFELVKGENESIPF
EEREWKLTMNGNTSFLTNGEEMNWTFMNAFEAGSDADIKARRAAESGKLKME"
gene <420098..>420778
/gene="YAF9"
/locus_tag="YNL107W"
/db_xref="GeneID:855616"
mRNA <420098..>420778
/gene="YAF9"
/locus_tag="YNL107W"
/product="YEATS domain-containing protein YAF9"
/transcript_id="NM_001182945.3"
/db_xref="GeneID:855616"
CDS 420098..420778
/gene="YAF9"
/locus_tag="YNL107W"
/experiment="EXISTENCE:direct assay:GO:0000812 Swr1
complex [PMID:14690608|PMID:14645854]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006281 DNA repair
[PMID:16135807]"
/experiment="EXISTENCE:direct assay:GO:0006338 chromatin
remodeling [PMID:14645854]"
/experiment="EXISTENCE:direct assay:GO:0035267 NuA4
histone acetyltransferase complex [PMID:15485911]"
/experiment="EXISTENCE:mutant phenotype:GO:0031509
subtelomeric heterochromatin formation [PMID:15485911]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:15302830]"
/note="Subunit of NuA4 histone H4 acetyltransferase and
SWR1 complexes; may function to antagonize silencing near
telomeres; interacts directly with Swc4p; has homology to
human leukemogenic protein AF9; contains a YEATS domain"
/codon_start=1
/product="YEATS domain-containing protein YAF9"
/protein_id="NP_014292.3"
/db_xref="GeneID:855616"
/db_xref="SGD:S000005051"
/translation="MAPTISKRIKTLSVSRPIIYGNTAKKMGSVKPPNAPAEHTHLWT
IFVRGPQNEDISYFIKKVVFKLHDTYPNPVRSIEAPPFELTETGWGEFDINIKVYFVE
EANEKVLNFYHRLRLHPYANPVPNSDNGNEQNTTDHNSKDAEVSSVYFDEIVFNEPNE
EFFKILMSRPGNLLPSNKTDDCVYSKQLEQEEIDRIEIGIEKVDKEIDELKQKLENLV
KQEAINGS"
gene complement(<420944..>424495)
/gene="INP52"
/locus_tag="YNL106C"
/gene_synonym="SJL2"
/db_xref="GeneID:855618"
mRNA complement(<420944..>424495)
/gene="INP52"
/locus_tag="YNL106C"
/gene_synonym="SJL2"
/product="phosphatidylinositol-3-/phosphoinositide
5-phosphatase INP52"
/transcript_id="NM_001182944.1"
/db_xref="GeneID:855618"
CDS complement(420944..424495)
/gene="INP52"
/locus_tag="YNL106C"
/gene_synonym="SJL2"
/EC_number="3.1.3.36"
/experiment="EXISTENCE:direct assay:GO:0004438
phosphatidylinositol-3-phosphate phosphatase activity
[PMID:10224048]"
/experiment="EXISTENCE:direct assay:GO:0004439
phosphatidylinositol-4,5-bisphosphate 5-phosphatase
activity [PMID:10224048]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11094088]"
/experiment="EXISTENCE:direct assay:GO:0030479 actin
cortical patch [PMID:15798181|PMID:11094088]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807]"
/experiment="EXISTENCE:direct assay:GO:0043812
phosphatidylinositol-4-phosphate phosphatase activity
[PMID:10224048]"
/experiment="EXISTENCE:direct assay:GO:0043813
phosphatidylinositol-3,5-bisphosphate 5-phosphatase
activity [PMID:10224048]"
/experiment="EXISTENCE:direct assay:GO:0046856
phosphatidylinositol dephosphorylation [PMID:10224048]"
/experiment="EXISTENCE:mutant phenotype:GO:0004439
phosphatidylinositol-4,5-bisphosphate 5-phosphatase
activity [PMID:9438131]"
/experiment="EXISTENCE:mutant phenotype:GO:0046856
phosphatidylinositol dephosphorylation [PMID:9438131]"
/experiment="EXISTENCE:physical interaction:GO:0030479
actin cortical patch [PMID:15798181]"
/note="Polyphosphatidylinositol phosphatase;
dephosphorylates a number of phosphatidylinositol
phosphates (PtdInsPs, PIPs) to PI; involved in
endocytosis; hyperosmotic stress causes translocation to
actin patches; synaptojanin-like protein with a Sac1
domain; INP52 has a paralog, INP53, that arose from the
whole genome duplication"
/codon_start=1
/product="phosphatidylinositol-3-/phosphoinositide
5-phosphatase INP52"
/protein_id="NP_014293.1"
/db_xref="GeneID:855618"
/db_xref="SGD:S000005050"
/translation="MKILLSKQQTRKIAIVSETHGLVFRPINSKNSRRSTCAVELVPK
AELNGNGFRRLSNHEIYGFIGLIEIEGLMFIATITGKSKVAQPIPNKTVNKIYAVDFF
CLNNSKWDFMDIDSSGYPIVTNDGDFAISSPPSISTHSSRSSLRSSSSRSLNAQEQAP
KHPCHELRKLLSNGSFYYSTDFDLTCTLQKRGFTEHSLSFDDFDREFMWNSFLMDEII
TYRDRLDVTAKELLDQRGFLTTVIRGFAETIFSYINRLKVGLTIISRQSWKRAGTRFN
ARGIDDDGHVANFVETEMIMYSSQYCYAFTQIRGSLPIFWEQDTSLISPKIQITRSVE
ATQPTFDEHFIRLFKKYGPVHIINLLSTKSSEIQLSRRYKEQLKNSEKMKIGRDVFLT
SFDFHRETSQDGFAAASRIIPKIRNTILDAGYFSYDVKEGRLISEQDGVFRTNCLDCL
DRTNLIQQTISLAVFKLFLEDFRLVKPSSFIDDNEFVQKVNALWADNGDQISQIYTGT
NALKSSYSRKGKMSFSGALSDATKSVSRMYINNFVDKGKQQNIDTLLGKLPHQQVVEL
YDPICEYVNERLLESEEKFTTHSNINLFVGTFNVNGNSRRADLSKWLFPIGDKFKPDV
VVLGLQEVIELTAGSILNADYTKSSFWETMVTDCLNQYEEKYLLLRVEQMSSLLILFF
ARSDRAYNIKEVGGSTKKTGFGGITGNKGAVAIRFDYGATSFCFVNTHLSAGASNIDE
RRNDYNNIYRNITFPRSKTIPHHDSLFWLGDLNYRITLTNDEVRRELRAQKDGYIDRL
LQYDQLTQEINEGVVFQGFKEPTLQFRPTYKYDYGTDNYDTSEKARTPSWTDRIIYKG
ENLHPLAYSDAPLKISDHKPVYAAYRANVKFVDEKEKLNLVEKLYAEYKNTHPEALTT
GPDELSHARMEKQKESIPLDATVQSAGIKLIDLDDTSSCVSPLLSGPSPQPSVVGPGG
LSNVSPDKSKLNVLPPPPPTSRHNKEPSSKLLSPTKEISIVSVSPRKGESNLPALERH
STPKPLPPVPALSLSKPVSLQKSSSELQHAKETIDNGKIVPRPCPPIRRKSSTAPDEI
STSTKNSGVSTTEDPEPAKASTKPEKPPVVKKPHYLSVAANKLNTSQEHSIKVSPSNS
KSEEELPCKKKSKPKVPAKNPELEKLSVHPLKPCDPN"
gene complement(<424895..>426754)
/gene="LEU4"
/locus_tag="YNL104C"
/db_xref="GeneID:855619"
mRNA complement(<424895..>426754)
/gene="LEU4"
/locus_tag="YNL104C"
/product="2-isopropylmalate synthase LEU4"
/transcript_id="NM_001182942.1"
/db_xref="GeneID:855619"
CDS complement(424895..426754)
/gene="LEU4"
/locus_tag="YNL104C"
/EC_number="2.3.3.13"
/experiment="EXISTENCE:direct assay:GO:0003852
2-isopropylmalate synthase activity
[PMID:25841022|PMID:3275644]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:3275644|PMID:11914276|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:16823961|PMID:3275644|PMID:24769239|PMID:11914276|PM
ID:14562095|PMID:14576278|PMID:25841022]"
/experiment="EXISTENCE:genetic interaction:GO:0009098
L-leucine biosynthetic process [PMID:10790691]"
/experiment="EXISTENCE:mutant phenotype:GO:0009098
L-leucine biosynthetic process [PMID:25841022]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:25841022]"
/note="Alpha-isopropylmalate synthase (2-isopropylmalate
synthase); the main isozyme responsible for the first step
in the leucine biosynthesis pathway; LEU4 has a paralog,
LEU9, that arose from the whole genome duplication"
/codon_start=1
/product="2-isopropylmalate synthase LEU4"
/protein_id="NP_014295.1"
/db_xref="GeneID:855619"
/db_xref="SGD:S000005048"
/translation="MVKESIIALAEHAASRASRVIPPVKLAYKNMLKDPSSKYKPFNA
PKLSNRKWPDNRITRAPRWLSTDLRDGNQSLPDPMSVEQKKEYFHKLVNIGFKEIEVS
FPSASQTDFDFTRYAVENAPDDVSIQCLVQSREHLIKRTVEALTGAKKATIHTYLATS
DMFREIVFNMSREEAISKAVEATKLVRKLTKDDPSQQATRWSYEFSPECFSDTPGEFA
VEICEAVKKAWEPTEENPIIFNLPATVEVASPNVYADQIEYFATHITEREKVCISTHC
HNDRGCGVAATELGMLAGADRVEGCLFGNGERTGNVDLVTVAMNMYTQGVSPNLDFSD
LTSVLDVVERCNKIPVSQRAPYGGDLVVCAFSGSHQDAIKKGFNLQNKKRAQGETQWR
IPYLPLDPKDIGRDYEAVIRVNSQSGKGGAAWVILRSLGLDLPRNMQIEFSSAVQDHA
DSLGRELKSDEISKLFKEAYNYNDEQYQAISLVNYNVEKFGTERRVFTGQVKVGDQIV
DIEGTGNGPISSLVDALSNLLNVRFAVANYTEHSLGSGSSTQAASYIHLSYRRNADNE
KAYKWGVGVSEDVGDSSVRAIFATINNIIHSGDVSIPSLAEVEGKNAAASGSA"
gene <427735..>429753
/gene="MET4"
/locus_tag="YNL103W"
/db_xref="GeneID:855620"
mRNA <427735..>429753
/gene="MET4"
/locus_tag="YNL103W"
/product="Met4p"
/transcript_id="NM_001182941.3"
/db_xref="GeneID:855620"
CDS 427735..429753
/gene="MET4"
/locus_tag="YNL103W"
/experiment="EXISTENCE:direct assay:GO:0003713
transcription coactivator activity [PMID:9799240]"
/experiment="EXISTENCE:direct assay:GO:0045944 positive
regulation of transcription by RNA polymerase II
[PMID:9799240]"
/experiment="EXISTENCE:direct assay:GO:0046685 response to
arsenic-containing substance [PMID:15689486]"
/experiment="EXISTENCE:direct assay:GO:0046686 response to
cadmium ion [PMID:15689486]"
/experiment="EXISTENCE:direct assay:GO:0089713
Cbf1-Met4-Met28 complex [PMID:9171357|PMID:8665859]"
/experiment="EXISTENCE:mutant phenotype:GO:0000096 sulfur
amino acid metabolic process [PMID:1549123]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:39138175|PMID:22696683]"
/experiment="EXISTENCE:physical interaction:GO:0005634
nucleus [PMID:9799240]"
/note="Leucine-zipper transcriptional activator; regulates
the sulfur amino acid pathway; requires different
combinations of auxiliary factors Cbf1p, Met28p, Met31p
and Met32p; specific involvement in sulfur
starvation-induced autophagy with Gcn4p; feedforward loop
exists in the regulation of genes controlled by Met4p and
Met32p; ubiquitinated by SCF-Met30p, leading to either
degradation or maintenance in an inactive state; regulates
degradation of its DNA-binding cofactors by SCF-Met30p
targeting"
/codon_start=1
/product="Met4p"
/protein_id="NP_014296.4"
/db_xref="GeneID:855620"
/db_xref="SGD:S000005047"
/translation="MKQEQSHEGDSYSTEFINLFGKDTATHPSSNNGANNNGMGSTNS
LDQFVATASSSSSLVTSSENRRPLIGDVTNRGNTNLYDHAVTPEILLEQLAYVDNFIP
SLDNEFSNVDWNVNTTHNNANNNGADTFSSINANPFDLDEQLAIELSAFADDSFIFPD
EDKPSNNNNNSNNGNDDHSNHDVLHEDPSTNNRQRNPHFLTQRRNTFLTSQYDQSKSR
FSSKNKRNGNNGETNNFGDNMQNNHPFEPNFMGSPSQFPADATNMTSIDHGGFTNVDI
TSTENNTTGDNGVDALSNLLHRTTHTPNRSSPLSNVTSAQNSSSQQRKHSESKVDSNS
DNNSSNKAPNITVPDYSIIPTSVLVTLLPRVNVPNGAYNSLISAGFDNDQIDAIAAIM
AYHHQKKIRENNSNNNKNINTNDSQEAPILKNINELLSVLIPPSPAETAAPTTLSTSP
SFNEHGVVAEASFLSSILELGIKHPKSNNIHNQRQPSRNDHKISRESDGNNGNDNVHH
NNAVIKSSTTRGDEIAKIRSEPTLNASSSDHKENSLKRSHSGDLKNKKVPVDRKYSDN
EDDEYDDADLHGFEKKQLIKKELGDDDEDLLIQSKKSHQKKKLKEKELESSIHELTEI
AASLQKRIHTLETENKLLKNLVLSSGETEGIKKAESLKKQIFEKVQKE"
gene <430087..>434493
/gene="POL1"
/locus_tag="YNL102W"
/gene_synonym="CDC17; CRT5; HPR3"
/db_xref="GeneID:855621"
mRNA <430087..>434493
/gene="POL1"
/locus_tag="YNL102W"
/gene_synonym="CDC17; CRT5; HPR3"
/product="DNA-directed DNA polymerase alpha catalytic
subunit POL1"
/transcript_id="NM_001182940.3"
/db_xref="GeneID:855621"
CDS 430087..434493
/gene="POL1"
/locus_tag="YNL102W"
/gene_synonym="CDC17; CRT5; HPR3"
/EC_number="2.7.7.7"
/experiment="EXISTENCE:direct assay:GO:0000510 H3-H4
histone complex chaperone activity [PMID:38713623]"
/experiment="EXISTENCE:direct assay:GO:0003887
DNA-directed DNA polymerase activity
[PMID:1704371|PMID:3888995|PMID:3907855]"
/experiment="EXISTENCE:direct assay:GO:0005657 replication
fork [PMID:15773893]"
/experiment="EXISTENCE:direct assay:GO:0005658 alpha DNA
polymerase:primase complex [PMID:3888995|PMID:22593576]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0006278
RNA-templated DNA biosynthetic process [PMID:17429354]"
/experiment="EXISTENCE:mutant phenotype:GO:0000510 H3-H4
histone complex chaperone activity [PMID:38713623]"
/experiment="EXISTENCE:mutant phenotype:GO:0000731 DNA
synthesis involved in DNA repair [PMID:2651896]"
/experiment="EXISTENCE:mutant phenotype:GO:0006260 DNA
replication [PMID:2651896]"
/experiment="EXISTENCE:mutant phenotype:GO:0006279
premeiotic DNA replication [PMID:2651896]"
/experiment="EXISTENCE:mutant phenotype:GO:0006302
double-strand break repair [PMID:10025407]"
/note="Catalytic subunit of the DNA polymerase I
alpha-primase complex; required for the initiation of DNA
replication during mitotic DNA synthesis and premeiotic
DNA synthesis; H3-H4 histone chaperone, downstream of
Pol32p, involved in the transfer of parental histones to
the lagging strand during DNA replication"
/codon_start=1
/product="DNA-directed DNA polymerase alpha catalytic
subunit POL1"
/protein_id="NP_014297.3"
/db_xref="GeneID:855621"
/db_xref="SGD:S000005046"
/translation="MSSKSEKLEKLRKLQAARNGTSIDDYEGDESDGDRIYDEIDEKE
YRARKRQELLHDDFVVDDDGVGYVDRGVEEDWREVDNSSSDEDTGNLASKDSKRKKNI
KREKDHQITDMLRTQHSKSTLLAHAKKSQKKSIPIDNFDDILGEFESGEVEKPNILLP
SKLRENLNSSPTSEFKSSIKRVNGNDESSHDAGISKKVKIDPDSSTDKYLEIESSPLK
LQSRKLRYANDVQDLLDDVENSPVVATKRQNVLQDTLLANPPSAQSLADEEDDEDSDE
DIILKRRTMRSVTTTRRVNIDSRSNPSTSPFVTAPGTPIGIKGLTPSKSLQSNTDVAT
LAVNVKKEDVVDPETDTFQMFWLDYCEVNNTLILFGKVKLKDDNCVSAMVQINGLCRE
LFFLPREGKTPTDIHEEIIPLLMDKYGLDNIRAKPQKMKYSFELPDIPSESDYLKVLL
PYQTPKSSRDTIPSDLSSDTFYHVFGGNSNIFESFVIQNRIMGPCWLDIKGADFNSIR
NASHCAVEVSVDKPQNITPTTTKTMPNLRCLSLSIQTLMNPKENKQEIVSITLSAYRN
ISLDSPIPENIKPDDLCTLVRPPQSTSFPLGLAALAKQKLPGRVRLFNNEKAMLSCFC
AMLKVEDPDVIIGHRLQNVYLDVLAHRMHDLNIPTFSSIGRRLRRTWPEKFGRGNSNM
NHFFISDICSGRLICDIANEMGQSLTPKCQSWDLSEMYQVTCEKEHKPLDIDYQNPQY
QNDVNSMTMALQENITNCMISAEVSYRIQLLTLTKQLTNLAGNAWAQTLGGTRAGRNE
YILLHEFSRNGFIVPDKEGNRSRAQKQRQNEENADAPVNSKKAKYQGGLVFEPEKGLH
KNYVLVMDFNSLYPSIIQEFNICFTTVDRNKEDIDELPSVPPSEVDQGVLPRLLANLV
DRRREVKKVMKTETDPHKRVQCDIRQQALKLTANSMYGCLGYVNSRFYAKPLAMLVTN
KGREILMNTRQLAESMNLLVVYGDTDSVMIDTGCDNYADAIKIGLGFKRLVNERYRLL
EIDIDNVFKKLLLHAKKKYAALTVNLDKNGNGTTVLEVKGLDMKRREFCPLSRDVSIH
VLNTILSDKDPEEALQEVYDYLEDIRIKVETNNIRIDKYKINMKLSKDPKAYPGGKNM
PAVQVALRMRKAGRVVKAGSVITFVITKQDEIDNAADTPALSVAERAHALNEVMIKSN
NLIPDPQYYLEKQIFAPVERLLERIDSFNVVRLSEALGLDSKKYFRREGGNNNGEDIN
NLQPLETTITDVERFKDTVTLELSCPSCDKRFPFGGIVSSNYYRVSYNGLQCKHCEQL
FTPLQLTSQIEHSIRAHISLYYAGWLQCDDSTCGIVTRQVSVFGKRCLNDGCTGVMRY
KYSDKQLYNQLLYFDSLFDCEKNKKQELKPIYLPDDLDYPKEQLTESSIKALTEQNRE
LMETGRSVVQKYLNDCGRRYVDMTSIFDFMLN"
gene <434999..>437140
/gene="AVT4"
/locus_tag="YNL101W"
/db_xref="GeneID:855622"
mRNA <434999..>437140
/gene="AVT4"
/locus_tag="YNL101W"
/product="Avt4p"
/transcript_id="NM_001182939.1"
/db_xref="GeneID:855622"
CDS 434999..437140
/gene="AVT4"
/locus_tag="YNL101W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:11274162]"
/experiment="EXISTENCE:direct assay:GO:1990816
vacuole-mitochondrion membrane contact site
[PMID:25026036]"
/experiment="EXISTENCE:genetic interaction:GO:0005302
L-tyrosine transmembrane transporter activity
[PMID:11274162]"
/experiment="EXISTENCE:genetic interaction:GO:0015186
L-glutamine transmembrane transporter activity
[PMID:11274162]"
/experiment="EXISTENCE:genetic interaction:GO:0015188
L-isoleucine transmembrane transporter activity
[PMID:11274162]"
/experiment="EXISTENCE:genetic interaction:GO:0032974
amino acid transmembrane export from vacuole
[PMID:11274162]"
/experiment="EXISTENCE:mutant phenotype:GO:0000329
fungal-type vacuole membrane [PMID:11274162]"
/experiment="EXISTENCE:mutant phenotype:GO:0005302
L-tyrosine transmembrane transporter activity
[PMID:11274162]"
/experiment="EXISTENCE:mutant phenotype:GO:0015186
L-glutamine transmembrane transporter activity
[PMID:11274162]"
/experiment="EXISTENCE:mutant phenotype:GO:0015188
L-isoleucine transmembrane transporter activity
[PMID:11274162]"
/experiment="EXISTENCE:mutant phenotype:GO:0032974 amino
acid transmembrane export from vacuole [PMID:11274162]"
/note="Vacuolar transporter; exports large neutral amino
acids from the vacuole; member of a family of seven S.
cerevisiae genes (AVT1-7) related to vesicular
GABA-glycine transporters; localizes to sites of contact
between the vacuole and mitochondria (vCLAMPs)"
/codon_start=1
/product="Avt4p"
/protein_id="NP_014298.1"
/db_xref="GeneID:855622"
/db_xref="SGD:S000005045"
/translation="MVTNNGDGEHLGIRRNGNLRHPSNNMKIPRRAQSTVLNSNPFYS
RKYSMSTLTPRDICRSVDSRVFVDMSSPNFQTLEDPHRDEIINSVRLNYLNSSKRSSV
SHGNEAIPRVNPTKNSSASTIAAANVDSDDDETNLSSAGGDITHDIYKLVKAEDPKRL
RRPRSMENVTPKIEHHTKLSSASGLNVPGGFRREFIVNKKRQEHQLNDSASSDFTSHE
SDSINQSSPSSNQDIDKVPFLTRNFLEFLYVFGHFAGESFEDDFIPDSSNMMIRGEDE
RSALLSRPDHMKVLPSAKGTTSTKKVFLILLKSFIGTGVLFLPNAFHNGGLFFSVSML
AFFGIYSYWCYYILVQAKSSCGVSSFGDIGLKLYGPWMRIIILFSLVITQVGFSGAYM
IFTAKNLQAFLDNVFHVGVLPLSYLMVFQTIIFIPLSFIRNISKLSLPSLLANFFIMA
GLVIVIIFTAKRLFFDLMGTPAMGVVYGLNADRWTLFIGTAIFAFEGIGLIIPVQDSM
RNPEKFPLVLALVILTATILFISIATLGYLAYGSNVQTVILLNLPQSNIFVNLIQLFY
SIAIMLSTPLQLFPAIKIIENKFFPKFTKIYVKHDDLTTRVELRPNSGKLNWKIKWLK
NFIRSIIVIIVVSIAYFGSDNLDKFVSVIGSLACIPLVYIYPSMLHLRGNSLPETKGE
FWRFKPMLDTILIFFGIASMLYTSYQSIFGV"
gene <437613..>438317
/gene="MIC27"
/locus_tag="YNL100W"
/gene_synonym="AIM37; MCS27"
/db_xref="GeneID:855623"
mRNA <437613..>438317
/gene="MIC27"
/locus_tag="YNL100W"
/gene_synonym="AIM37; MCS27"
/product="Mic27p"
/transcript_id="NM_001182938.1"
/db_xref="GeneID:855623"
CDS 437613..438317
/gene="MIC27"
/locus_tag="YNL100W"
/gene_synonym="AIM37; MCS27"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:14562095|PMID:16823961|PMID:24769239|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0044284
mitochondrial crista junction [PMID:22009199]"
/experiment="EXISTENCE:direct assay:GO:0061617 MICOS
complex [PMID:21944719|PMID:21987634|PMID:22009199]"
/experiment="EXISTENCE:mutant phenotype:GO:0032461
positive regulation of protein oligomerization
[PMID:29733859]"
/experiment="EXISTENCE:mutant phenotype:GO:0042407 cristae
formation [PMID:22009199|PMID:21987634|PMID:21944719]"
/experiment="EXISTENCE:mutant phenotype:GO:0043933
protein-containing complex organization [PMID:26968360]"
/note="Component of the MICOS complex; MICOS (formerly
MINOS or MitOS), a mitochondrial inner membrane complex
that extends into the intermembrane space with a role in
the maintenance of crista junctions, inner membrane
architecture, and formation of contact sites to the outer
membrane; forms a subcomplex with Mic10p and Mic12p whose
assembly and stability requires cardiolipin; positive
regulator of Mic10p oligomerization along with
cardiolipin; orthologous to human APOO and APOOL"
/codon_start=1
/product="Mic27p"
/protein_id="NP_014299.1"
/db_xref="GeneID:855623"
/db_xref="SGD:S000005044"
/translation="MVNFYDDVDESKSHGEFPLIPVVLQNSSELSVRTIPTGNEIIES
VHLTKWLRKYRNALASQLDRYEKGWQSKIANFRLQVQHVINYSRKNIFNVDSENKHTV
VPGSLIALGAFFAGSIAVNRSNWGAKRLIFGHKSSILEKLCTSLPSRILLPWVLAAAT
FKYWAPQTSQNLVNATENDLLPADFVKSYHNTWKRIYEEGYVAKKCDLKRQIDQTLQK
NIRYAREQLYEKLEQA"
gene complement(<438567..>439283)
/gene="OCA1"
/locus_tag="YNL099C"
/db_xref="GeneID:855624"
mRNA complement(<438567..>439283)
/gene="OCA1"
/locus_tag="YNL099C"
/product="putative tyrosine protein phosphatase OCA1"
/transcript_id="NM_001182937.3"
/db_xref="GeneID:855624"
CDS complement(438567..439283)
/gene="OCA1"
/locus_tag="YNL099C"
/EC_number="3.1.3.48"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0016791 phosphatase
activity [PMID:40212001]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress [PMID:11408586]"
/note="Putative protein tyrosine phosphatase; contains
phosphatase activity using the non-specific phosphatase
substrate, pNPP, and is inhibited by sodium orthovanadate;
required for cell cycle arrest in response to oxidative
damage of DNA"
/codon_start=1
/product="putative tyrosine protein phosphatase OCA1"
/protein_id="NP_014300.3"
/db_xref="GeneID:855624"
/db_xref="SGD:S000005043"
/translation="MTSKVGEYEDVPEDESRLTEENVSVPEEEVEDEDEEEDDDDDHI
YINEETESGREKVLVSHAPQERIVPPLNFCPVERYLYRSGQPSPVNFPFLLNLKLKTI
IWLSNEEPQDTLLEFCDTHRINLQFAAINPDAGEDDNPWDGLTEHSIINVLQTIVTQE
NYPLLVCCGMGRHRTGTVIGCLRRIMGWNLASVSEEYRRFTGSRGGRILVELLIEAFD
TNLVKIDKNKAPSWLLTALE"
gene complement(<439602..>440570)
/gene="RAS2"
/locus_tag="YNL098C"
/gene_synonym="CTN5; CYR3; GLC5; TSL7"
/db_xref="GeneID:855625"
mRNA complement(<439602..>440570)
/gene="RAS2"
/locus_tag="YNL098C"
/gene_synonym="CTN5; CYR3; GLC5; TSL7"
/product="Ras family GTPase RAS2"
/transcript_id="NM_001182936.1"
/db_xref="GeneID:855625"
CDS complement(439602..440570)
/gene="RAS2"
/locus_tag="YNL098C"
/gene_synonym="CTN5; CYR3; GLC5; TSL7"
/EC_number="3.6.5.2"
/experiment="EXISTENCE:direct assay:GO:0003924 GTPase
activity [PMID:8106517]"
/experiment="EXISTENCE:direct assay:GO:0005525 GTP binding
[PMID:6438624]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:23127800]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:22575457]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:22575457]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane
[PMID:16622836|PMID:20162532|PMID:23127800|PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0016236
macroautophagy [PMID:15016820]"
/experiment="EXISTENCE:genetic interaction:GO:0097271
protein localization to bud neck [PMID:12782684]"
/experiment="EXISTENCE:mutant phenotype:GO:0000411
positive regulation of transcription by galactose
[PMID:16292676]"
/experiment="EXISTENCE:mutant phenotype:GO:0010603
regulation of cytoplasmic mRNA processing body assembly
[PMID:21925385]"
/experiment="EXISTENCE:mutant phenotype:GO:0030437
ascospore formation [PMID:2558958]"
/experiment="EXISTENCE:mutant phenotype:GO:0032258
cytoplasm to vacuole targeting by the Cvt pathway
[PMID:15016820]"
/experiment="EXISTENCE:mutant phenotype:GO:0032880
regulation of protein localization [PMID:15917658]"
/experiment="EXISTENCE:mutant phenotype:GO:0042149
cellular response to glucose starvation [PMID:21925385]"
/experiment="EXISTENCE:mutant phenotype:GO:2000222
positive regulation of pseudohyphal growth
[PMID:1547504|PMID:8643578]"
/note="GTP-binding protein; regulates nitrogen starvation
response, sporulation, and filamentous growth;
farnesylation and palmitoylation required for activity and
localization to plasma membrane; activated by increased
levels of glycolysis intermediate
fructose-1,6-bisphosphate; homolog of mammalian Ras
proto-oncogenes; RAS2 has a paralog, RAS1, that arose from
the whole genome duplication"
/codon_start=1
/product="Ras family GTPase RAS2"
/protein_id="NP_014301.1"
/db_xref="GeneID:855625"
/db_xref="SGD:S000005042"
/translation="MPLNKSNIREYKLVVVGGGGVGKSALTIQLTQSHFVDEYDPTIE
DSYRKQVVIDDEVSILDILDTAGQEEYSAMREQYMRNGEGFLLVYSITSKSSLDELMT
YYQQILRVKDTDYVPIVVVGNKSDLENEKQVSYQDGLNMAKQMNAPFLETSAKQAINV
EEAFYTLARLVRDEGGKYNKTLTENDNSKQTSQDTKGSGANSVPRNSGGHRKMSNAAN
GKNVNSSTTVVNARNASIESKTGLAGNQATNGKTQTDRTNIDNSTGQAGQANAQSANT
VNNRVNNNSKAGQVSNAKQARKQQAAPGGNTSEASKSGSGGCCIIS"
gene complement(<440797..>440919)
/gene="PLS1"
/locus_tag="YNL097C-B"
/gene_synonym="YNL097C-A"
/db_xref="GeneID:1466513"
mRNA complement(<440797..>440919)
/gene="PLS1"
/locus_tag="YNL097C-B"
/gene_synonym="YNL097C-A"
/product="Pls1p"
/transcript_id="NM_001184620.1"
/db_xref="GeneID:1466513"
CDS complement(440797..440919)
/gene="PLS1"
/locus_tag="YNL097C-B"
/gene_synonym="YNL097C-A"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:35563734]"
/experiment="EXISTENCE:mutant phenotype:GO:1902986
regulation of lysine biosynthetic process via aminoadipic
acid [PMID:35563734]"
/note="Peroxisomal matrix protein that regulates lysine
biosynthesis; localization to peroxisomes upon lysine
deprivation is dependent on targeting factor Pex5p and
lysine biosynthesis enzyme Lys1p; affects abundance of
Lys1p and the lysine biosynthesis pathway"
/codon_start=1
/product="Pls1p"
/protein_id="NP_878155.1"
/db_xref="GeneID:1466513"
/db_xref="SGD:S000028699"
/translation="MTAKTKQSWNKGIWENGKQGSHQQTFLPKIWVNIYSTPTS"
gene complement(<441366..>442358)
/gene="PHO23"
/locus_tag="YNL097C"
/db_xref="GeneID:855626"
mRNA complement(<441366..>442358)
/gene="PHO23"
/locus_tag="YNL097C"
/product="Pho23p"
/transcript_id="NM_001182935.3"
/db_xref="GeneID:855626"
CDS complement(441366..442358)
/gene="PHO23"
/locus_tag="YNL097C"
/experiment="EXISTENCE:direct assay:GO:0000118 histone
deacetylase complex [PMID:12672825]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0033698 Rpd3L
complex
[PMID:19040720|PMID:16314178|PMID:16286007|PMID:16286008]"
/experiment="EXISTENCE:direct assay:GO:0070210
Rpd3L-Expanded complex [PMID:19040720]"
/experiment="EXISTENCE:direct assay:GO:0140002 histone
H3K4me3 reader activity [PMID:17142463]"
/experiment="EXISTENCE:mutant phenotype:GO:0006325
chromatin organization [PMID:10805724]"
/experiment="EXISTENCE:mutant phenotype:GO:0016479
negative regulation of transcription by RNA polymerase I
[PMID:19270272]"
/experiment="EXISTENCE:mutant phenotype:GO:0034605
cellular response to heat [PMID:20398213]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:17210643|PMID:20398213]"
/experiment="EXISTENCE:mutant phenotype:GO:0061186
negative regulation of silent mating-type cassette
heterochromatin formation [PMID:16286008]"
/experiment="EXISTENCE:mutant phenotype:GO:0061188
negative regulation of rDNA heterochromatin formation
[PMID:16286008]"
/experiment="EXISTENCE:mutant phenotype:GO:2000219
positive regulation of invasive growth in response to
glucose limitation [PMID:18202364]"
/note="Component of the Rpd3L histone deacetylase complex;
involved in transcriptional regulation of PHO5; affects
termination of snoRNAs and cryptic unstable transcripts
(CUTs); C-terminus shares significant sequence identity
with the human candidate tumor suppressor p33-ING1 and its
isoform ING3"
/codon_start=1
/product="Pho23p"
/protein_id="NP_014302.3"
/db_xref="GeneID:855626"
/db_xref="SGD:S000005041"
/translation="MSSPANLFPGLNDITDVLEEFPLATSRYLTLLHEIDAKCVHSMP
NLNERIDKFLKKDFNKDHQTQVRLLNNINKIYEELMPSLEEKMHVSSIMLDNLDRLTS
RLELAYEVAIKNTEIPRGLRLGVDNHPAMHLHHELMEKIESKSNSKSSQALKSESRRE
AMAANRRQGEHYSASTHQQDDSKNDANYGGSRHESQDHTGNNTNSRKRANAANTNNAD
PETKKRKRRVATTAVSPSTISTATAVNNGRIGTSTASRGVSSVGNSNNSRISRPKTND
YGEPLYCYCNQVAYGEMVGCDGADCELEWFHLPCIGLETLPKGKWYCDDCKKKL"
gene 443006..443119
/locus_tag="YNCN0007W"
/db_xref="GeneID:855627"
tRNA join(443006..443043,443076..443119)
/locus_tag="YNCN0007W"
/product="tRNA-Leu"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:855627"
/db_xref="SGD:S000006645"
gene complement(<443398..>444315)
/gene="RPS7B"
/locus_tag="YNL096C"
/db_xref="GeneID:855628"
mRNA complement(join(<443398..443826,444172..>444315))
/gene="RPS7B"
/locus_tag="YNL096C"
/product="40S ribosomal protein eS7 RPS7B"
/transcript_id="NM_001182934.3"
/db_xref="GeneID:855628"
CDS complement(join(443398..443826,444172..444315))
/gene="RPS7B"
/locus_tag="YNL096C"
/experiment="EXISTENCE:direct assay:GO:0032040
small-subunit processome [PMID:15590835]"
/experiment="EXISTENCE:genetic interaction:GO:0042254
ribosome biogenesis [PMID:16246728]"
/note="Protein component of the small (40S) ribosomal
subunit; interacts with Kti11p; deletion causes
hypersensitivity to zymocin; homologous to mammalian
ribosomal protein S7, no bacterial homolog; RPS7B has a
paralog, RPS7A, that arose from the whole genome
duplication; protein abundance increases in response to
DNA replication stress"
/codon_start=1
/product="40S ribosomal protein eS7 RPS7B"
/protein_id="NP_014303.3"
/db_xref="GeneID:855628"
/db_xref="SGD:S000005040"
/translation="MSSVQSKILSQAPSELELQVAKTFIDLESSSPELKADLRPLQIK
SIREIDVTGGKKALVLFVPVPALSAYHKVQTKLTRELEKKFPDRHVIFLAERRILPKP
SRTSRQVQKRPRSRTLTAVHDKVLEDMVFPTEIVGKRVRYLVGGNKIQKVLLDSKDVQ
QIDYKLESFQAVYNKLTGKQIVFEIPSQTN"
gene complement(<444912..>446840)
/locus_tag="YNL095C"
/db_xref="GeneID:855629"
mRNA complement(<444912..>446840)
/locus_tag="YNL095C"
/product="uncharacterized protein"
/transcript_id="NM_001182933.1"
/db_xref="GeneID:855629"
CDS complement(444912..446840)
/locus_tag="YNL095C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/note="hypothetical protein; predicted to contain a
transmembrane domain; not an essential gene; YNL095C has a
paralog, ECM3, that arose from the whole genome
duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014304.1"
/db_xref="GeneID:855629"
/db_xref="SGD:S000005039"
/translation="MVHITLGQAIWVSVKPIIKIYLIIGVGFLMAKMGILTVEATRII
SDIVLTVLLPSLSFNKIVANIEDKDIKSVGIICLSALLIFGSGFFFAYVVRLFLPVPK
QWYGGILAGGMFPNISDLPIAYLQSMDQGLVFSEEEGNKGVANVIIFLTMFLICIFNL
GGFRLIESDFEYNDDESAVRVSETTKTQPAVSANTTNTDTSERFFSNEQQLFNNKYTA
RDSLTEAIGTKGENADVPPISRRSTNSIAPLSLPDTSSNSKITKPVQVKARNTIACTQ
SEESQATRGSNPLDSQSSASTIHSYNTSESYESSIDTMRARRTASQPRAYNTTTLLEE
NCLDEKCPKNMSMAALEPIRSIDMRALPSQNIHHLIREYSNVDQYGHQRRNSSLRGAD
MNDVHSISSNSTLQTIKTANLTRILTSDATVSKKDIETSGESLPQWMRKFSLTPLLVF
FLKNCLRPCSMAVIIALTVAFIPWVKALFVTTANTPHISQAPDNAPPLSFFMDFTGYV
GAACVPFGLILLGATLGRLKIGNLYPGFWKAAVTLVILRQCVMPIFGVLWCDRLVKAG
WVNWQDDRMLLFVIAISWNLPTMTTLIYFTASFTPPETTAPIQMECVSFFLMLQYPLM
VVSLPFLVSYFLKVQMNL"
gene <447611..>449374
/gene="APP1"
/locus_tag="YNL094W"
/db_xref="GeneID:855630"
mRNA <447611..>449374
/gene="APP1"
/locus_tag="YNL094W"
/product="phosphatidate phosphatase APP1"
/transcript_id="NM_001182932.1"
/db_xref="GeneID:855630"
CDS 447611..449374
/gene="APP1"
/locus_tag="YNL094W"
/EC_number="3.1.3.4"
/experiment="EXISTENCE:direct assay:GO:0008195
phosphatidate phosphatase activity
[PMID:23335564|PMID:23071111]"
/experiment="EXISTENCE:direct assay:GO:0030479 actin
cortical patch [PMID:11489916]"
/experiment="EXISTENCE:mutant phenotype:GO:0006629 lipid
metabolic process [PMID:23071111]"
/experiment="EXISTENCE:mutant phenotype:GO:0008195
phosphatidate phosphatase activity [PMID:23071111]"
/note="Phosphatidate phosphatase, converts phosphatidate
to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are
responsible for all the phosphatidate phosphatase
activity; component of cortical actin patches; interacts
with components of endocytic pathway"
/codon_start=1
/product="phosphatidate phosphatase APP1"
/protein_id="NP_014305.1"
/db_xref="GeneID:855630"
/db_xref="SGD:S000005038"
/translation="MNSQGYDESSSSTAATSGPTSGDPRMGKKQRFMNLIRTTKDVYI
PNLTSSISQKTMDGIRSTTNSFEGYNDLPMELPHNTTITYFPTYTTTNLVDPDGLSAP
RKDFETTVRCAVSYPGNPTSRRNRWLLSLCKQYLRTGTAEADVAPVVPPHLEEDSGDL
NDSQSSIESSLSSKSENRYSHMGIQEEDVLNERIQGFLSKKVPNTPVVVDLLPKDKLR
GDTASFFGTTDSYGNLLIKAETDFLPSKINITLDTPIEGHADPISETFPANYVSPYGI
GLISDIDDTIKHTGVTGDRRSMFRNVFIHDVQSWVIDGVPLWYKTLHDVADVDFFYVS
NSPIQTFTLLKQYICANFPPGPIFLKQYSGNFFSTIMTSSANRKIQPIANILKDFPKK
KFILVGDSGEHDLEAYTTTALQFPNQILAIYIRCCSNSMSDVPSHDEEVMNEVNNIIE
LQQRPMQMTKSTVRTRRRPPPPPIPSTQKPSLTEEQTESIRMSRRNKDENNAKRVAPP
PLPNRQLPNLDANTYYVPSSQNDYGMYGAFMDKKADEWKRRVMDSIQKLSNQDTTLMF
FSDPALSLEDSIRRIREKYSN"
rep_origin 449429..449588
/note="ARS1419; Autonomously Replicating Sequence"
/db_xref="SGD:S000118387"
gene <449868..>450530
/gene="YPT53"
/locus_tag="YNL093W"
/db_xref="GeneID:855631"
mRNA <449868..>450530
/gene="YPT53"
/locus_tag="YNL093W"
/product="Rab family GTPase YPT53"
/transcript_id="NM_001182931.3"
/db_xref="GeneID:855631"
CDS 449868..450530
/gene="YPT53"
/locus_tag="YNL093W"
/experiment="EXISTENCE:direct assay:GO:0003924 GTPase
activity [PMID:11210571]"
/experiment="EXISTENCE:genetic interaction:GO:0006895
Golgi to endosome transport [PMID:22748138]"
/experiment="EXISTENCE:mutant phenotype:GO:0003924 GTPase
activity [PMID:8163546]"
/experiment="EXISTENCE:mutant phenotype:GO:0005770 late
endosome [PMID:11872141]"
/experiment="EXISTENCE:mutant phenotype:GO:0006623 protein
targeting to vacuole [PMID:8163546]"
/experiment="EXISTENCE:mutant phenotype:GO:0006897
endocytosis [PMID:8163546]"
/note="Stress-induced Rab family GTPase; required for
vacuolar protein sorting and endocytosis; involved in
ionic stress tolerance; similar to Vps21p and Ypt52p;
functional homolog of Vps21p; mammalian Rab5 homolog;
YPT53 has a paralog, VPS21, that arose from the whole
genome duplication"
/codon_start=1
/product="Rab family GTPase YPT53"
/protein_id="NP_014306.3"
/db_xref="GeneID:855631"
/db_xref="SGD:S000005037"
/translation="MDKHTAAIPTLTIKVVLLGESAVGKSSIVLRFVSDDFKESKEPT
IGAAFLTKRITRDGKVIKFEIWDTAGQERFAPLAPMYYRNAQAALVVFDVTNEGSFYK
AQNWVEELHEKVGHDIVIALVGNKMDLLNNDDENENRAMKAPAVQNLCERENLLYFEA
SAKTGENIYQIFQTLGEKVPCPEQNTRQSSTHDRTITDNQRIDLESTTVESTRETGGC
NC"
gene <450871..>452073
/locus_tag="YNL092W"
/db_xref="GeneID:855632"
mRNA <450871..>452073
/locus_tag="YNL092W"
/product="S-adenosylmethionine-dependent
methyltransferase"
/transcript_id="NM_001182930.1"
/db_xref="GeneID:855632"
CDS 450871..452073
/locus_tag="YNL092W"
/EC_number="2.1.1.22"
/experiment="EXISTENCE:direct assay:GO:0006479 protein
methylation [PMID:24651469]"
/experiment="EXISTENCE:direct assay:GO:0008757
S-adenosylmethionine-dependent methyltransferase activity
[PMID:24651469]"
/note="S-adenosylmethionine-dependent protein
methyltransferase; capable of automethylation; member of
the seven beta-strand family; YNL092W is not an essential
gene"
/codon_start=1
/product="S-adenosylmethionine-dependent
methyltransferase"
/protein_id="NP_014307.1"
/db_xref="GeneID:855632"
/db_xref="SGD:S000005036"
/translation="MDENEFDNQRENKAVARVIISFLKYEEYALKEIYNLRVKKWASI
SDRQKDMVPNYTKYLANLKAAIIENGKFFRSVAEYALQSISFEPGEIVQPNDLDMSKT
CSLLTQVYREWSAEAISERNCLNSRLVPFLKTLSPPKADILIPGCGTGRLLVDLSRMG
YNCEGNEFSYHMLLVSQYMLNAGLLQNQIIIYPFIHCFSHWKKIEDQLSPIKVPDIEA
WSSNKGMGSMSICAGSFVDCYGRNQGTKISSHYTFSRRMQLSRAKAENSKDVVVTNFF
IDTGSNILDYLDTIGHVLKPGGIWCNFGPLLYHFENDHGVETTYEVNPYSGFQDKIND
YTPLMGLELSSDDIISIATNHLDFELIRRESGILCGYGRYAGPESCAMPGYMCHYWIL
KSNPTNES"
gene <452408..>456130
/gene="NST1"
/locus_tag="YNL091W"
/db_xref="GeneID:855633"
mRNA <452408..>456130
/gene="NST1"
/locus_tag="YNL091W"
/product="Nst1p"
/transcript_id="NM_001182929.1"
/db_xref="GeneID:855633"
CDS 452408..456130
/gene="NST1"
/locus_tag="YNL091W"
/experiment="EXISTENCE:direct assay:GO:0000932 P-body
[PMID:34347506]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0017148
negative regulation of translation [PMID:34347506]"
/experiment="EXISTENCE:mutant phenotype:GO:0009651
response to salt stress [PMID:11816027]"
/note="Translational inhibitor and suppressor of
proteasome-assembly defects; P-body component that
inhibits translation, rescues proteasome base assembly
defects and aggregation of regulatory particle subunits
during stress when overexpressed; proposed to function to
bridge the HOG and pheromone pathways with the cell wall
integrity pathway in response to heat stress; interacts
physically and genetically with splicing factor MSL1;
large, highly-charge protein containing many disordered
regions"
/codon_start=1
/product="Nst1p"
/protein_id="NP_014308.1"
/db_xref="GeneID:855633"
/db_xref="SGD:S000005035"
/translation="MPPNSKSKRRKNKSKQHNKKNGNSDPEQSINPTQLVPRMEPELY
HTESDYPTSRVIKRAPNGDVIVEPINTDDDKKERTANLTHNKDSMDSASSLAFTLDSH
WESLSPEEKKTILRIEKEEVFNVIRNYQDDHSCSCSVCGRRHLAMDQEMERIYNTLYA
MDKDKDPETNPIKFHLGIIKELQISKNQQQNDLSSTKGEVVKNFLSSSTVGSLKEEVL
HFKQKQLSKQEQAHNETADNTSLLEENLNNIHINKTSSEISANFNSVSDEELQQKYSN
FTKTFISSHPKIAEEYVQKMMMYPNIRALTDDLMNSNGQGFLNAIEDFVRDGQIQASK
KDDSITEDEASSTDLTDPKEFTTMLHSGKPLTEDEYADLQRNIAERMTNAYDTASKKF
KDVSQLEKELFTRFMSGRDKKSFRELIIQSFKNKFDGELGPSVLAATLSSCFSSQSKD
TSLDTDSIYEDEDEEDYDDYSEYAEDSEEVSEYEGIEAVEKPEHDEKSNGIRETLHLS
YDHDHKRQNHPHHHYHSTSTHSEDELSEEEYISDIELPHDPHKHFHRDDDILDGDEDE
PEEEDENEGDDEEDTYDSGLDETDRLEEGRKLIQIAITKLLQSRIMASYHEKQADNNR
LKLLQELEEEKRKKREKEEKKQKKREKEKEKKRLQQLAKEEEKRKREEEKERLKKELE
EREMRRREAQRKKVEEAKRKKDEERKRRLEEQQRREEMQEKQRKQKEELKRKREEEKK
RIREQKRLEQEKLQKEKEEEERQRLIAEDALRKQKLNEEQTSANILSAKPFTENGVGN
PVSSQSHPNMTNYQEDNSCSINDEILKMVNSVAASKPVSPTGFNVHDLLLPSTNNQMP
AMEQSHLPQPGNQNNHFGTTTIPNALDLATKSSLQTENNYLMNSQTLENTSLLMHNNS
SPTKLLPNDFGLSSWGGLTNTMSINPTCKPPVIQTSEMESQAHKSSPQATMPSFGLPN
GGTHRKSFTDELNTLTSMLSSSGFADTSLSSSGFPPSQRSVWNDQKSSFSGPSTAGNF
NNSSIQSGMLLAPTLGSVESFPNRTSIWDSSTTPMMNKSELSGRNITSTAQDSPAFMA
SNIWSSNSQYNSPYLTSNVLQSPQISSGVDESHILDSIYNTYLAISPQDSLNPYIAIG
TLFQNLVGLNLDYSTFINKLISMQGAYNCEFFTDNNGSITHVRFARQTPAGHSKGLLN
QLFSGLNDPTATPFTSRPHTSTRASFPIASSTTQTS"
gene <456565..>457143
/gene="RHO2"
/locus_tag="YNL090W"
/db_xref="GeneID:855634"
mRNA <456565..>457143
/gene="RHO2"
/locus_tag="YNL090W"
/product="Rho family GTPase RHO2"
/transcript_id="NM_001182928.3"
/db_xref="GeneID:855634"
CDS 456565..457143
/gene="RHO2"
/locus_tag="YNL090W"
/EC_number="3.6.5.2"
/experiment="EXISTENCE:direct assay:GO:0003924 GTPase
activity [PMID:11591390|PMID:10526184]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:18417612]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="Non-essential small GTPase of the Rho/Rac family of
Ras-like proteins; involved in the establishment of cell
polarity and in microtubule assembly"
/codon_start=1
/product="Rho family GTPase RHO2"
/protein_id="NP_014309.3"
/db_xref="GeneID:855634"
/db_xref="SGD:S000005034"
/translation="MSEKAVRRKLVIIGDGACGKTSLLYVFTLGKFPEQYHPTVFENY
VTDCRVDGIKVSLTLWDTAGQEEYERLRPFSYSKADIILIGFAVDNFESLINARTKWA
DEALRYCPDAPIVLVGLKKDLRQEAHFKENATDEMVPIEDAKQVARAIGAKKYMECSA
LTGEGVDDVFEVATRTSLLMKKEPGANCCIIL"
gene <457704..>461990
/gene="TOP2"
/locus_tag="YNL088W"
/gene_synonym="TOR3; TRF3"
/db_xref="GeneID:855636"
mRNA <457704..>461990
/gene="TOP2"
/locus_tag="YNL088W"
/gene_synonym="TOR3; TRF3"
/product="DNA topoisomerase 2"
/transcript_id="NM_001182926.3"
/db_xref="GeneID:855636"
CDS 457704..461990
/gene="TOP2"
/locus_tag="YNL088W"
/gene_synonym="TOR3; TRF3"
/EC_number="5.6.2.2"
/experiment="EXISTENCE:direct assay:GO:0000795
synaptonemal complex [PMID:1315786]"
/experiment="EXISTENCE:direct assay:GO:0003918 DNA
topoisomerase type II (double strand cut, ATP-hydrolyzing)
activity [PMID:6088500|PMID:6323017]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:6088500|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0006265 DNA
topological change [PMID:6088500]"
/experiment="EXISTENCE:direct assay:GO:0097047 DNA
replication termination region [PMID:20797631]"
/experiment="EXISTENCE:genetic interaction:GO:0000722
telomere maintenance via recombination [PMID:25430478]"
/experiment="EXISTENCE:genetic interaction:GO:0009303 rRNA
transcription [PMID:2436053]"
/experiment="EXISTENCE:mutant phenotype:GO:0000019
regulation of mitotic recombination [PMID:2902925]"
/experiment="EXISTENCE:mutant phenotype:GO:0006265 DNA
topological change [PMID:31969709]"
/experiment="EXISTENCE:mutant phenotype:GO:0006271 DNA
strand elongation involved in DNA replication
[PMID:2549254]"
/experiment="EXISTENCE:mutant phenotype:GO:0006325
chromatin organization [PMID:9199287]"
/experiment="EXISTENCE:mutant phenotype:GO:0007131
reciprocal meiotic recombination [PMID:2156624]"
/experiment="EXISTENCE:mutant phenotype:GO:0034080 CENP-A
containing chromatin assembly [PMID:18701701]"
/experiment="EXISTENCE:mutant phenotype:GO:0097046
replication fork progression beyond termination site
[PMID:20797631]"
/note="Topoisomerase II; relieves torsional strain in DNA
by cleaving and re-sealing phosphodiester backbone of both
positively and negatively supercoiled DNA; cleaves
complementary strands; localizes to axial cores in
meiosis; required for replication slow zone (RSZ) breakage
following Mec1p inactivation; human homolog TOP2A
implicated in cancers, and can complement yeast null
mutant"
/codon_start=1
/product="DNA topoisomerase 2"
/protein_id="NP_014311.3"
/db_xref="GeneID:855636"
/db_xref="SGD:S000005032"
/translation="MSTEPVSASDKYQKISQLEHILKRPDTYIGSVETQEQLQWIYDE
ETDCMIEKNVTIVPGLFKIFDEILVNAADNKVRDPSMKRIDVNIHAEEHTIEVKNDGK
GIPIEIHNKENIYIPEMIFGHLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTEFILET
ADLNVGQKYVQKWENNMSICHPPKITSYKKGPSYTKVTFKPDLTRFGMKELDNDILGV
MRRRVYDINGSVRDINVYLNGKSLKIRNFKNYVELYLKSLEKKRQLDNGEDGAAKSDI
PTILYERINNRWEVAFAVSDISFQQISFVNSIATTMGGTHVNYITDQIVKKISEILKK
KKKKSVKSFQIKNNMFIFINCLIENPAFTSQTKEQLTTRVKDFGSRCEIPLEYINKIM
KTDLATRMFEIADANEENALKKSDGTRKSRITNYPKLEDANKAGTKEGYKCTLVLTEG
DSALSLAVAGLAVVGRDYYGCYPLRGKMLNVREASADQILKNAEIQAIKKIMGLQHRK
KYEDTKSLRYGHLMIMTDQDHDGSHIKGLIINFLESSFPGLLDIQGFLLEFITPIIKV
SITKPTKNTIAFYNMPDYEKWREEESHKFTWKQKYYKGLGTSLAQEVREYFSNLDRHL
KIFHSLQGNDKDYIDLAFSKKKADDRKEWLRQYEPGTVLDPTLKEIPISDFINKELIL
FSLADNIRSIPNVLDGFKPGQRKVLYGCFKKNLKSELKVAQLAPYVSECTAYHHGEQS
LAQTIIGLAQNFVGSNNIYLLLPNGAFGTRATGGKDAAAARYIYTELNKLTRKIFHPA
DDPLYKYIQEDEKTVEPEWYLPILPMILVNGAEGIGTGWSTYIPPFNPLEIIKNIRHL
MNDEELEQMHPWFRGWTGTIEEIEPLRYRMYGRIEQIGDNVLEITELPARTWTSTIKE
YLLLGLSGNDKIKPWIKDMEEQHDDNIKFIITLSPEEMAKTRKIGFYERFKLISPISL
MNMVAFDPHGKIKKYNSVNEILSEFYYVRLEYYQKRKDHMSERLQWEVEKYSFQVKFI
KMIIEKELTVTNKPRNAIIQELENLGFPRFNKEGKPYYGSPNDEIAEQINDVKGATSD
EEDEESSHEDTENVINGPEELYGTYEYLLGMRIWSLTKERYQKLLKQKQEKETELENL
LKLSAKDIWNTDLKAFEVGYQEFLQRDAEARGGNVPNKGSKTKGKGKRKLVDDEDYDP
SKKNKKSTARKGKKIKLEDKNFERILLEQKLVTKSKAPTKIKKEKTPSVSETKTEEEE
NAPSSTSSSSIFDIKKEDKDEGELSKISNKFKKISTIFDKMGSTSATSKENTPEQDDV
ATKKNQTTAKKTAVKPKLAKKPVRKQQKVVELSGESDLEILDSYTDREDSNKDEDDAI
PQRSRRQRSSRAASVPKKSYVETLELSDDSFIEDDEEENQGSDVSFNEED"
gene <462411..>465947
/gene="TCB2"
/locus_tag="YNL087W"
/db_xref="GeneID:855637"
mRNA <462411..>465947
/gene="TCB2"
/locus_tag="YNL087W"
/product="tricalbin"
/transcript_id="NM_001182925.1"
/db_xref="GeneID:855637"
CDS 462411..465947
/gene="TCB2"
/locus_tag="YNL087W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005933 cellular
bud [PMID:13679573]"
/experiment="EXISTENCE:direct assay:GO:0008289 lipid
binding [PMID:22250200]"
/experiment="EXISTENCE:direct assay:GO:0032541 cortical
endoplasmic reticulum [PMID:22250200]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0055091
phospholipid homeostasis [PMID:35440494]"
/experiment="EXISTENCE:genetic interaction:GO:0060304
regulation of phosphatidylinositol dephosphorylation
[PMID:23237950]"
/experiment="EXISTENCE:genetic interaction:GO:0090158
endoplasmic reticulum membrane organization
[PMID:23237950]"
/experiment="EXISTENCE:mutant phenotype:GO:0035621 ER to
Golgi ceramide transport [PMID:33205016]"
/note="Lipid-binding ER tricalbin involved in ER-plasma
membrane tethering; one of at least 7 proteins (Ice2p,
Ist2p, Scs2/Scs22p, Tcb1-Tcb3p) that affect cER tethering
and contact with the plasma membrane (PM); regulates PI4P
levels by controlling access of Sac1p to its substrate
PI4P in the PM; regulates PM phospholipid homeostasis;
contains 3 calcium and lipid binding domains; localizes to
the cortical ER, the cell periphery and is enriched in bud
membranes; mRNA is targeted to the bud tip"
/codon_start=1
/product="tricalbin"
/protein_id="NP_014312.1"
/db_xref="GeneID:855637"
/db_xref="SGD:S000005031"
/translation="MSPNSSKTRTDQISSMPGINEATKVESKNVVKDAVPIKSEVETN
GTSIVREKQDPSYVGWKQVGGWEEKDELTSEDLLVDVNKDTFLGNLLPDKFYGDWYHE
VAILIIAGLCSFVLGYFKFSLASVLIVMLTTGMLYRTSSKKYRESLRDLAQKEQTVEK
ITSDYESVEWLNTFLDKYWPIIEPSVSQQIVDGTNTALSENVAIPKFIKAIWLDQFTL
GVKPPRIDAIKTFQNTKSDVVVMDVCLSFTPHDMSDLDAKQCRNYVNSNVVLKAKIFG
MDIPVSVADIFFQVFVRFRFQLMTTLPLVETINIQLLEVPEVDFISRLLGNSVFNWEI
LAIPGLMRLIQKMAFKYLSPVLLPPFSLQLNIPQLLSKTGLPIGVLEIKVKNAHGLRK
LVGMIKKTVDPYLTFELSGKIVGKTKVFKNSANPVWNESIYILLQSFTDPLTIAVYDK
RETLSDKKMGTVIFNLNKLHANHYHKNEKVHFLRNSKPVGELTFDLRFFPTIEPKKLL
NGDEEPLPDMNTGITKITIRELKGLDELSDKKFVFAELYVNAELVMTTKKEKRTAHLK
WNSDYYSVVTDRRKTICRFVLKDQSGKVISSSVQPLNHLIDRTEVNKEWIPLRNGKGE
LKVTTYWRPVDIDLGLKSVGYTTPIGMLRVFINKAENLRNPDSLGKISPYAKVSVNGV
ARGRTNERIETLNPIWNQSIYVSVTSPLQKVSIDCFGIDTNGDDHNLGSLNIQTQNIY
HKDNDDKYTIFIDNAPRTGNLIGKKGVKGTVTYYLSFYPVVPVLSLEEAKEVDEINEK
KDKLEKQKSTLDDKNISKEEKERIKKEEFRLTEKYDMYSYKMKLDLDELLQYNAGVLG
VTVLGGELPQPGLYVQTFFDSCGYAAITSAKNAIRTIKTGWSGDFMIKELEWSVTTFR
VTKTKDANKAENFICEVNIPTIELVRNCYYKPSVLNLIGKKSAKLLVQVSWFPVTATE
LPQSDLITNSGDLKITAKSAENLIGVNKNGYSDPYVEFFLNEKSTSPFFKTAVQKKTL
NPTWNESKTIEVSNRVNDYLTINVKDYESTNSNRSIGKAVVPLSTIDPESDTTFNIPL
VGPKGEDGGVLHLEFEFEPRYTTNVVKREAGLGNFATKGLGTGIKAGSTVFALGTNVV
STGLGTIDKVKAGVFGGKKSTTTGDKKSEEKQ"
gene <466334..>466642
/gene="SNN1"
/locus_tag="YNL086W"
/db_xref="GeneID:855638"
mRNA <466334..>466642
/gene="SNN1"
/locus_tag="YNL086W"
/product="Snn1p"
/transcript_id="NM_001182924.1"
/db_xref="GeneID:855638"
CDS 466334..466642
/gene="SNN1"
/locus_tag="YNL086W"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0031083 BLOC-1
complex [PMID:23547030]"
/experiment="EXISTENCE:mutant phenotype:GO:0007032
endosome organization [PMID:23547030]"
/experiment="EXISTENCE:mutant phenotype:GO:0032880
regulation of protein localization [PMID:23547030]"
/note="Subunit of the BLOC-1 complex involved in endosomal
maturation; interacts with Msb3p; green fluorescent
protein (GFP)-fusion protein localizes to endosomes"
/codon_start=1
/product="Snn1p"
/protein_id="NP_014313.1"
/db_xref="GeneID:855638"
/db_xref="SGD:S000005030"
/translation="MAGDSISADGTGVHPVELSVYSVLSTDLDGLYQSINELRESQAL
LILMLRKVRDKLRREGQVLYDPEPFKPTMDKLADLSARVRILSQRYEELQGNARALNN
"
gene <467131..>469623
/gene="MKT1"
/locus_tag="YNL085W"
/db_xref="GeneID:855639"
mRNA <467131..>469623
/gene="MKT1"
/locus_tag="YNL085W"
/product="Mkt1p"
/transcript_id="NM_001182923.3"
/db_xref="GeneID:855639"
CDS 467131..469623
/gene="MKT1"
/locus_tag="YNL085W"
/experiment="EXISTENCE:direct assay:GO:0000932 P-body
[PMID:24970865]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405]"
/experiment="EXISTENCE:direct assay:GO:0034399 nuclear
periphery [PMID:25817432]"
/experiment="EXISTENCE:mutant phenotype:GO:0006974 DNA
damage response [PMID:18617998]"
/note="Putative nuclease that forms a complex with Pbp1p;
involved in posttranscriptional regulation of HO,
propagation of M2 dsRNA satellite of L-A virus; allelic
variation affects mitochondrial genome stability, drug
resistance, stress responses through posttranscriptional
modulation of Puf3p targets; forms cytoplasmic foci upon
DNA replication stress; localization to P-bodies under
ethanol stress differs between strains"
/codon_start=1
/product="Mkt1p"
/protein_id="NP_014314.3"
/db_xref="GeneID:855639"
/db_xref="SGD:S000005029"
/translation="MAIKSLESFLFERGLVGSYAIEALNNCTLDIDVNHYVSRLLTNK
REQYLDAIGGFPTSLKMYLESDLKIFKDFNITPIFVFNGGLTYNQLEASGHFTAASAS
ASISSTTTSSSGTNATTRSNTESVLLQRSRGWTQWNNLISSNQNSYIDQPIQPQEPFR
HNTTIDSKAYQNDLIAYFIEHGYMYQVAPYSSWFQLAYLLNSAYIDAIYGPTDCLMLD
CVDRFILGMEFPNKEFRFIDRSRVMKDLGCTHEEFIDIAMAVGNDLQPTTLPPLQIYP
VPQLFDIALEMVLNTGTNFYAYQLSTTLQNDSKENIQNYQRGISALRYMPVLKDTGKV
ELFVQEIVVSEEDSEKNNKDGKKSNLSSPSSASSSASPATTVTKNASEKLTYEKSSTK
EVRKPRDIPNDVHDFIGQMLPHEYYFYRSIGLVTGKLFDAIVTGVYPEEPPLGGGSST
SYKKLVSKSVEIFKNKEINLLTQPINRYYQIKQIKQVKWYAANEPTTLTNRMSPSMFE
TINHLIVKTETSDEKEFSISEFITTINGSSNMAKDFISEKVIFPNSVPIESKLNSPFN
LLSTNFLRLLVLLEFFTFDFKEKLLEPTRWGEVFLKLNELNIDSKYHESVIIFLVFLK
CDVLKLDEEVQPPAPSALSQATLRSYPEESLYVLLITRVLTLFQVDQKPSNYHGPIDK
KTLIFRDHLSFIKENLNELFEAVLISSLTSGEFNRLSLDNFGWARKIVRYLPFKLDSP
NTIMAMMWEFFLQKYLHNGNAKNDALSLVATEFNTYKSTPNLDEQFVESHRFLLEISK
VMQELNAAKLIDENVFKLFTKAVEFTTTALSS"
gene complement(<470053..>471102)
/gene="END3"
/locus_tag="YNL084C"
/db_xref="GeneID:855640"
mRNA complement(<470053..>471102)
/gene="END3"
/locus_tag="YNL084C"
/product="End3p"
/transcript_id="NM_001182922.1"
/db_xref="GeneID:855640"
CDS complement(470053..471102)
/gene="END3"
/locus_tag="YNL084C"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:1990964 actin
cytoskeleton-regulatory complex
[PMID:10594004|PMID:17151356]"
/experiment="EXISTENCE:genetic interaction:GO:0061709
reticulophagy [PMID:35101986]"
/experiment="EXISTENCE:mutant phenotype:GO:0006897
endocytosis [PMID:8380177]"
/experiment="EXISTENCE:mutant phenotype:GO:0030476
ascospore wall assembly [PMID:15944351]"
/experiment="EXISTENCE:physical interaction:GO:0030674
protein-macromolecule adaptor activity [PMID:10594004]"
/experiment="EXISTENCE:physical interaction:GO:1990964
actin cytoskeleton-regulatory complex [PMID:17151356]"
/note="EH domain-containing protein involved in
endocytosis; actin cytoskeletal organization and cell wall
morphogenesis; forms a complex with Sla1p and Pan1p"
/codon_start=1
/product="End3p"
/protein_id="NP_014315.1"
/db_xref="GeneID:855640"
/db_xref="SGD:S000005028"
/translation="MPKLEQFEIKKYWQIFSGLKPIENKVNHDQVLPILYNSKLDSSV
LNKIWFLADIDDDDNLDFEEFVICMRLIFDMVNKNISSVPDELPDWLIPGSKVNLIKE
RKKRKQIENADLPPKKEIKVDWYMSPDDLNQYEKIYNSCAKLTDGTITFNELSTKLST
KFFNISKTDLNKVWSLINPQNLPSIDRDPTFYFIHCLRQRNDLGAEIPASLPNSLAEV
CNKKQLSYDLRSSQPPTKRKEEANEVDNLRDNGQNSSSDSSGSNVLSNEDSIKQKYAS
LTDDQVANMREQLEGLLNYKKSEKTQGGSKLSKRINIRSITDDLDNIEQQVEVLENYL
NNKRHELQALQAEIN"
gene <471377..>472861
/gene="SAL1"
/locus_tag="YNL083W"
/db_xref="GeneID:855641"
mRNA <471377..>472861
/gene="SAL1"
/locus_tag="YNL083W"
/product="Ca(2+)-binding ATP:ADP antiporter SAL1"
/transcript_id="NM_001182921.1"
/db_xref="GeneID:855641"
CDS 471377..472861
/gene="SAL1"
/locus_tag="YNL083W"
/experiment="EXISTENCE:direct assay:GO:0005509 calcium ion
binding [PMID:15238515]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:mutant phenotype:GO:0005471 ATP:ADP
antiporter activity [PMID:18485069]"
/experiment="EXISTENCE:mutant phenotype:GO:0006839
mitochondrial transport [PMID:18485069]"
/experiment="EXISTENCE:mutant phenotype:GO:0015866 ADP
transport [PMID:18485069]"
/experiment="EXISTENCE:mutant phenotype:GO:0015867 ATP
transport [PMID:18485069]"
/note="ADP/ATP transporter; member of the Ca2+-binding
subfamily of mitochondrial carriers, with two EF-hand
motifs; transport activity of either Sal1p or Pet9p is
critical for viability; polymorphic in different S.
cerevisiae strains"
/codon_start=1
/product="Ca(2+)-binding ATP:ADP antiporter SAL1"
/protein_id="NP_014316.3"
/db_xref="GeneID:855641"
/db_xref="SGD:S000005027"
/translation="MLLKNCETDKQRDIRYACLFKELDVKGNGQVTLDNLISAFEKND
HPLKGNDEAIKMLFTAMDVNKDSVVDLSDFKKYASNAESQIWNGFQRIDLDHDGKIGI
NEINRYLSDLDNQSICNNELNHELSNEKMNKFSRFFEWAFPKRKANIALRGQASHKKN
TDNDRSKKTTDSDLYVTYDQWRDFLLLVPRKQGSRLHTAYSYFYLFNEDVDLSSEGDV
TLINDFIRGFGFFIAGGISGVISRTCTAPFDRLKVFLIARTDLSSILLNSKTDLLAKN
PNADINKISSPLAKAVKSLYRQGGIKAFYVGNGLNVIKVFPESSIKFGSFEVTKKIMT
KLEGCRDTKDLSKFSTYIAGGLAGMAAQFSVYPIDTLKFRVQCAPLDTKLKGNNLLFQ
TAKDMFREGGGQIILQRCHSRYSGHISLCCIRFGDFFCLKKMVYCQTGKDPEPTTRSG
HSKQPGCTSNGCIQWNCRSFCCLSNQSFKNKTTSPRNICTSLCV"
gene <473391..>476012
/gene="PMS1"
/locus_tag="YNL082W"
/db_xref="GeneID:855642"
mRNA <473391..>476012
/gene="PMS1"
/locus_tag="YNL082W"
/product="ATP-binding mismatch repair protein"
/transcript_id="NM_001182920.3"
/db_xref="GeneID:855642"
CDS 473391..476012
/gene="PMS1"
/locus_tag="YNL082W"
/experiment="EXISTENCE:direct assay:GO:0000404
heteroduplex DNA loop binding [PMID:9368761]"
/experiment="EXISTENCE:direct assay:GO:0003690
double-stranded DNA binding [PMID:11575920]"
/experiment="EXISTENCE:direct assay:GO:0003697
single-stranded DNA binding [PMID:11575920]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:11717305]"
/experiment="EXISTENCE:direct assay:GO:0032135 DNA
insertion or deletion binding [PMID:9368761]"
/experiment="EXISTENCE:direct assay:GO:0032139
dinucleotide insertion or deletion binding [PMID:9368761]"
/experiment="EXISTENCE:mutant phenotype:GO:0000710 meiotic
mismatch repair [PMID:16702432]"
/experiment="EXISTENCE:mutant phenotype:GO:0005524 ATP
binding [PMID:10938116]"
/experiment="EXISTENCE:mutant phenotype:GO:0006298
mismatch repair [PMID:2685551]"
/experiment="EXISTENCE:mutant phenotype:GO:0016887 ATP
hydrolysis activity [PMID:11717305]"
/experiment="EXISTENCE:physical interaction:GO:0032389
MutLalpha complex
[PMID:10570173|PMID:8066446|PMID:11237696]"
/note="ATP-binding protein required for mismatch repair;
required for both mitosis and meiosis; functions as a
heterodimer with Mlh1p; binds double- and single-stranded
DNA via its N-terminal domain; required for silencing at
the silent mating-type loci and telomeres; similar to E.
coli MutL"
/codon_start=1
/product="ATP-binding mismatch repair protein"
/protein_id="NP_014317.4"
/db_xref="GeneID:855642"
/db_xref="SGD:S000005026"
/translation="MTQIHQINDIDVHRITSGQVITDLTTAVKELVDNSIDANANQIE
IIFKDYGLESIECSDNGDGIDPSNYEFLALKHYTSKIAKFQDVAKVQTLGFRGEALSS
LCGIAKLSVITTTSPPKADKLEYDMVGHITSKTTTSRNKGTTVLVSQLFHNLPVRQKE
FSKTFKRQFTKCLTVIQGYAIINAAIKFSVWNITPKGKKNLILSTMRNSSMRKNISSV
FGAGGMRGLEEVDLVLDLNPFKNRMLGKYTDDPDFLDLDYKIRVKGYISQNSFGCGRN
SKDRQFIYVNKRPVEYSTLLKCCNEVYKTFNNVQFPAVFLNLELPMSLIDVNVTPDKR
VILLHNERAVIDIFKTTLSDYYNRQELALPKRMCSQSEQQAQKRLKTEVFDDRSTTHE
SDNENYHTARSESNQSNHAHFNSTTGVIDKSNGTELTSVMDGNYTNVTDVIGSECEVS
VDSSVVLDEGNSSTPTKKLPSIKTDSQNLSDLNLNNFSNPEFQNITSPDKARSLEKVV
EEPVYFDIDGEKFQEKAVLSQADGLVFVDNECHEHTNDCCHQERRGSTDTEQDDEADS
IYAEIEPVEINVRTPLKNSRKSISKDNYRSLSDGLTHRKFEDEILEYNLSTKNFKEIS
KNGKQMSSIISKRKSEAQENIIKNKDELEDFEQGEKYLTLTVSKNDFKKMEVVGQFNL
GFIIVTRKVDNKYDLFIVDQHASDEKYNFETLQAVTVFKSQKLIIPQPVELSVIDELV
VLDNLPVFEKNGFKLKIDEEEEFGSRVKLLSLPTSKQTLFDLGDFNELIHLIKEDGGL
RRDNIRCSKIRSMFAMRACRSSIMIGKPLNKKTMTRVVHNLSELDKPWNCPHGRPTMR
HLMELRDWSSFSKDYEI"
gene complement(<476188..>476619)
/gene="SWS2"
/locus_tag="YNL081C"
/gene_synonym="uS13m"
/db_xref="GeneID:855643"
mRNA complement(<476188..>476619)
/gene="SWS2"
/locus_tag="YNL081C"
/gene_synonym="uS13m"
/product="mitochondrial 37S ribosomal protein uS13m SWS2"
/transcript_id="NM_001182919.3"
/db_xref="GeneID:855643"
CDS complement(476188..476619)
/gene="SWS2"
/locus_tag="YNL081C"
/gene_synonym="uS13m"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:28154081|PMID:12392552]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:28154081]"
/experiment="EXISTENCE:direct assay:GO:0005763
mitochondrial small ribosomal subunit [PMID:28154081]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:36164978]"
/note="Component of mitochondrial small ribosomal subunit;
similar to E. coli S13 ribosomal protein; participates in
controlling sporulation efficiency; localizes to vacuole
in response to H2O2; localizes to peroxisomes in
glucose-growing cells"
/codon_start=1
/product="mitochondrial 37S ribosomal protein uS13m SWS2"
/protein_id="NP_014318.3"
/db_xref="GeneID:855643"
/db_xref="SGD:S000005025"
/translation="MVVHILGKGFKGKEVIKIALASKFYGIGKTTAEKICSKLGFYPW
MRMHQLSEPQIMSIASELSTMTIEGDARAIVKDNIALKRKIGSYSGMRHTLHLPVRGQ
HTRNNAKTARKLNKIDRRGIHTFSQAKVQHNPSLWSCIFGK"
gene complement(<476932..>478032)
/gene="EOS1"
/locus_tag="YNL080C"
/db_xref="GeneID:855644"
mRNA complement(<476932..>478032)
/gene="EOS1"
/locus_tag="YNL080C"
/product="Eos1p"
/transcript_id="NM_001182918.1"
/db_xref="GeneID:855644"
CDS complement(476932..478032)
/gene="EOS1"
/locus_tag="YNL080C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:17187761]"
/experiment="EXISTENCE:mutant phenotype:GO:0006487 protein
N-linked glycosylation [PMID:17187761]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress [PMID:17187761]"
/note="Protein involved in N-glycosylation; deletion
mutation confers sensitivity to exidative stress and shows
synthetic lethality with mutations in the spindle
checkpoint genes BUB3 and MAD1; YNL080C is not an
essential gene"
/codon_start=1
/product="Eos1p"
/protein_id="NP_014319.1"
/db_xref="GeneID:855644"
/db_xref="SGD:S000005024"
/translation="MTWILSTGMGPHEDKYAKHERATFKKTYSSMKTLSLNHLTAKQH
MLMALCRDISLLPPLTYIFTSLRKAWRVSMRTSITLYEPQSLRDAFTYFWQKLNSAYD
NNSSFEGASQKAVNGDGKDSLLLSALTTARASEYLLCSLWCLVSLYLSYAILDSLMVR
WIVKYSTVAAILRMFSMSLIIVTLELLLLSSLSPELDYFLHTWILISCVLTAVYIWQS
YLTSDLRYIRNQEGEVQEDTNVPEETEDYEDGEDDADEDSHVVVADESTVDVPSNDSL
SDNSDGGLFPVNRPSVSHSQSPKRPKKYPKKAFNFTTKRTIDLYKITVLCVVPVGLAS
FITMLGLLRNLFIQRLDVEQLERILHEMHPPA"
gene complement(<478566..>479165)
/gene="TPM1"
/locus_tag="YNL079C"
/db_xref="GeneID:855645"
mRNA complement(<478566..>479165)
/gene="TPM1"
/locus_tag="YNL079C"
/product="tropomyosin TPM1"
/transcript_id="NM_001182917.1"
/db_xref="GeneID:855645"
CDS complement(478566..479165)
/gene="TPM1"
/locus_tag="YNL079C"
/experiment="EXISTENCE:direct assay:GO:0000142 cellular
bud neck contractile ring [PMID:9864365]"
/experiment="EXISTENCE:direct assay:GO:0003786 actin
lateral binding [PMID:11457840]"
/experiment="EXISTENCE:direct assay:GO:0005884 actin
filament [PMID:7844152]"
/experiment="EXISTENCE:direct assay:GO:0032432 actin
filament bundle [PMID:9864365|PMID:2649250]"
/experiment="EXISTENCE:genetic interaction:GO:0051017
actin filament bundle assembly [PMID:9864365]"
/experiment="EXISTENCE:genetic interaction:GO:1903475
mitotic actomyosin contractile ring assembly
[PMID:12419188]"
/experiment="EXISTENCE:mutant phenotype:GO:0007015 actin
filament organization
[PMID:7844152|PMID:17237521|PMID:2649250]"
/experiment="EXISTENCE:mutant phenotype:GO:0007124
pseudohyphal growth [PMID:9055077]"
/experiment="EXISTENCE:mutant phenotype:GO:0030447
filamentous growth [PMID:15645503]"
/experiment="EXISTENCE:mutant phenotype:GO:0071474
cellular hyperosmotic response [PMID:17237521]"
/note="Major isoform of tropomyosin; binds to and
stabilizes actin cables and filaments, which direct
polarized cell growth and the distribution of several
organelles; acetylated by the NatB complex and acetylated
form binds actin most efficiently; TPM1 has a paralog,
TPM2, that arose from the whole genome duplication"
/codon_start=1
/product="tropomyosin TPM1"
/protein_id="NP_014320.1"
/db_xref="GeneID:855645"
/db_xref="SGD:S000005023"
/translation="MDKIREKLSNLKLEAESWQEKYEELKEKNKDLEQENVEKENQIK
SLTVKNQQLEDEIEKLEAGLSDSKQTEQDNVEKENQIKSLTVKNHQLEEEIEKLEAEL
AESKQLSEDSHHLQSNNDNFSKKNQQLEEDLEESDTKLKETTEKLRESDLKADQLERR
VAALEEQREEWERKNEELTVKYEDAKKELDEIAASLENL"
gene <479768..>480991
/gene="NIS1"
/locus_tag="YNL078W"
/gene_synonym="JIP1"
/db_xref="GeneID:855646"
mRNA <479768..>480991
/gene="NIS1"
/locus_tag="YNL078W"
/gene_synonym="JIP1"
/product="Nis1p"
/transcript_id="NM_001182916.3"
/db_xref="GeneID:855646"
CDS 479768..480991
/gene="NIS1"
/locus_tag="YNL078W"
/gene_synonym="JIP1"
/experiment="EXISTENCE:direct assay:GO:0005621 cellular
bud scar [PMID:25416945|PMID:30341817]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11817649]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:11817649|PMID:27385335]"
/experiment="EXISTENCE:direct assay:GO:0032153 cell
division site [PMID:25416945]"
/experiment="EXISTENCE:direct assay:GO:0032174 cellular
bud neck septin collar [PMID:30341817]"
/experiment="EXISTENCE:direct assay:GO:0032177 cellular
bud neck split septin rings [PMID:30341817]"
/experiment="EXISTENCE:mutant phenotype:GO:0005634 nucleus
[PMID:30341817]"
/experiment="EXISTENCE:mutant phenotype:GO:0007120 axial
cellular bud site selection [PMID:29074565]"
/experiment="EXISTENCE:mutant phenotype:GO:0045184
establishment of protein localization [PMID:29074565]"
/experiment="EXISTENCE:physical interaction:GO:0140014
mitotic nuclear division [PMID:11817649]"
/note="Protein involved in axial bud site selection;
prevents repolarization of cells at previous division
sites; recruited with Nba1p to the cell cortex and to
cytokinesis remnants (bud scars) by Aim44p and Nap1p where
Nba1p prevents Rsr1p-mediated activation of Cdc24p, and
therefore Cdc42p activation; acts with Nba1p to establish
transient bud scar localization of Rga1p, a Cdc42p GAP;
localizes to the nucleus and to cell division site during
G2/M, and is then stably recruited to cytokinesis
remnants"
/codon_start=1
/product="Nis1p"
/protein_id="NP_014321.3"
/db_xref="GeneID:855646"
/db_xref="SGD:S000005022"
/translation="METYETSIGTQSYPPTLFPPPLGTGGFTTSGYIHALVDSTSNSN
SNSNTNSNTNSNTNSNSDTKIPIVQISDDSHITHDSFKPYMEYHDASHLRNRNISKAD
QVESTEVMEQFTQWSNYKMRSRSPTINAKPIRHTSQRRTDFTSKNELSKFSKNHNFIF
HKGFLKRQHSIRREDRQAKVRSRFRSKKELTSVLNYIELEQMDIANVLASQPVNLHAI
RNLTSRDPAVTPIPFLRSQMYATSSRPPYLRNRSISRKLPKSQPGSLPTTMPATATKT
IKQNSTTPTTRSVYNKNVGRSNTSPSVLYHPKRRGKLNTKSHARKEQLLLELWREYLM
LVITQRTQLRLTLLCSPGSASNESSVCSSNASDLDMSLLSTPSSLFQMAGETKSNPII
IPDSQDDSILSSDPF"
gene <481391..>482977
/gene="APJ1"
/locus_tag="YNL077W"
/db_xref="GeneID:855647"
mRNA <481391..>482977
/gene="APJ1"
/locus_tag="YNL077W"
/product="Apj1p"
/transcript_id="NM_001182915.1"
/db_xref="GeneID:855647"
CDS 481391..482977
/gene="APJ1"
/locus_tag="YNL077W"
/experiment="EXISTENCE:direct assay:GO:0001671 ATPase
activator activity [PMID:23329686]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0034399 nuclear
periphery [PMID:25817432]"
/experiment="EXISTENCE:direct assay:GO:0042405 nuclear
inclusion body [PMID:32492414]"
/experiment="EXISTENCE:genetic interaction:GO:0016925
protein sumoylation [PMID:23329686]"
/experiment="EXISTENCE:mutant phenotype:GO:0043335 protein
unfolding [PMID:32492414]"
/experiment="EXISTENCE:mutant phenotype:GO:0051082
unfolded protein binding [PMID:11923285]"
/note="Hsp40 chaperone with a role in SUMO-mediated
protein degradation; works in concert with Hsp70 and
Hsp110 (Sse1p) to promote disaggregation of intranuclear
protein inclusions; competes with Hsp104 in
disaggregation, supporting turnover instead of refolding;
member of DnaJ-like family, conserved across eukaryotes;
overexpression interferes with propagation of the [Psi+]
prion; forms nuclear foci upon DNA replication stress"
/codon_start=1
/product="Apj1p"
/protein_id="NP_014322.1"
/db_xref="GeneID:855647"
/db_xref="SGD:S000005021"
/translation="MQQNTSLYDSLNVTAAASTSEIKKAYRNAALKYHPDKNNHTEES
KRKFQEICQAYEILKDNRLRALYDQYGTTDEVLIQEQQAQAQRQQAGPFSSSSNFDTE
AMSFPDLSPGDLFAQFFNSSATPSSNGSKSSFNFSFNNSSTPSFSFVNGSGVNNLYSS
SAKYNSNDEDHHLDRGPDIKHNLKCTLKELYMGKTAKLGLNRTRICSVCDGHGGLKKC
TCKTCKGQGIQTQTRRMGPLVQSWSQTCADCGGAGVFVKNKDICQQCQGLGFIKERKI
LQVTVQPGSCHNQLIVLTGEGDEVISTKGGGHEKVIPGDVVITILRLKDPNFQVINYS
NLICKKCKIDFMTSLCGGVVYIEGHPSGKLIKLDIIPGEILKPGCFKTVEDMGMPKFI
NGVRSGFGHLYVKFDVTYPERLEPENAKKIQNILANDKYIKAERSTMETADSDCYCDL
EKSYDSVEEHVLSSFEAPNLNNEVIEDDDLGDLINERDSRKRNNRRFDESNINNNNET
KRNKYSSPVSGFYDHDINGY"
gene <483556..>485310
/gene="MKS1"
/locus_tag="YNL076W"
/gene_synonym="LYS80"
/db_xref="GeneID:855648"
mRNA <483556..>485310
/gene="MKS1"
/locus_tag="YNL076W"
/gene_synonym="LYS80"
/product="Mks1p"
/transcript_id="NM_001182914.1"
/db_xref="GeneID:855648"
CDS 483556..485310
/gene="MKS1"
/locus_tag="YNL076W"
/gene_synonym="LYS80"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:10511541]"
/experiment="EXISTENCE:genetic interaction:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:11882290]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:11882290]"
/experiment="EXISTENCE:mutant phenotype:GO:0006808
regulation of nitrogen utilization [PMID:10511541]"
/experiment="EXISTENCE:mutant phenotype:GO:2000220
regulation of pseudohyphal growth [PMID:22043304]"
/experiment="EXISTENCE:physical interaction:GO:0031930
mitochondria-nucleus signaling pathway [PMID:14536080]"
/note="Pleiotropic negative transcriptional regulator;
involved in Ras-cAMP and lysine biosynthetic pathways and
nitrogen regulation; involved in retrograde (RTG)
mitochondria-to-nucleus signaling"
/codon_start=1
/product="Mks1p"
/protein_id="NP_014323.1"
/db_xref="GeneID:855648"
/db_xref="SGD:S000005020"
/translation="MSREAFDVPNIGTNKFLKVTPNLFTPERLNLFDDVELYLTLIKA
SKCVEQGERLHNISWRILNKAVLKEHNINRSKKRDGVKNIYYVLNPNNKQPIKPKQAA
VKQPPLQKANLPPTTAKQNVLTRPMTSPAIAQGAHDRSLDNPNSTNNDVKNDVAPNRQ
FSKSTTSGLFSNFADKYQKMKNVNHVANKEEPQTIITGFDTSTVITKKPLQSRRSRSP
FQHIGDMNMNCIDNETSKSTSPTLENMGSRKSSFPQKESLFGRPRSYKNDQNGQLSLS
KTSSRKGKNKIFFSSEDEDSDWDSVSNDSEFYADEDDEEYDDYNEEEADQYYRRQWDK
LLFAKNQQNLDSTKSSVSSANTINSNTSHDPVRKSLLSGLFLSEANSNSNNHNTAHSE
YASKHVSPTPQSSHSNIGPQPQQNPPSANGIKQQKPSLKTSNVTALASLSPPQPSNNE
RLSMDIQKDFKTDNESNHLYESNAPLTAQTILPTALSTHMFLPNNIHQQRMAIATGSN
TRHRFSRRQSMDIPSKNRNTGFLKTRMEISEEEKMVRTISRLDNTSIANSNGNGNDDT
SNQRTEALGRKTSNGGRI"
gene <485607..>486479
/gene="IMP4"
/locus_tag="YNL075W"
/db_xref="GeneID:855649"
mRNA <485607..>486479
/gene="IMP4"
/locus_tag="YNL075W"
/product="snoRNA-binding rRNA-processing protein IMP4"
/transcript_id="NM_001182913.3"
/db_xref="GeneID:855649"
CDS 485607..486479
/gene="IMP4"
/locus_tag="YNL075W"
/experiment="EXISTENCE:direct assay:GO:0006364 rRNA
processing [PMID:23980203]"
/experiment="EXISTENCE:direct assay:GO:0030515 snoRNA
binding [PMID:15489263]"
/experiment="EXISTENCE:direct assay:GO:0030686 90S
preribosome [PMID:12150911]"
/experiment="EXISTENCE:direct assay:GO:0032040
small-subunit processome [PMID:12242301|PMID:12068309]"
/experiment="EXISTENCE:direct assay:GO:0034457 Mpp10
complex [PMID:17515605]"
/experiment="EXISTENCE:direct assay:GO:0042134 rRNA
primary transcript binding [PMID:11864606]"
/experiment="EXISTENCE:direct assay:GO:0042274 ribosomal
small subunit biogenesis [PMID:23980203]"
/experiment="EXISTENCE:direct assay:GO:0043047
single-stranded telomeric DNA binding [PMID:17803460]"
/experiment="EXISTENCE:direct assay:GO:0140691 RNA folding
chaperone [PMID:15489263]"
/note="Subunit of the MPP10 chaperone complex and the
small subunit (SSU) processome; contains both U3 snoRNA
and rRNA primary transcript binding activity, as well as
RNA chaperone activity; SSU processome is required for
pre-18S rRNA processing; interacts with Mpp10p; member of
a superfamily of proteins that contain a sigma(70)-like
motif and associate with RNAs"
/codon_start=1
/product="snoRNA-binding rRNA-processing protein IMP4"
/protein_id="NP_014324.3"
/db_xref="GeneID:855649"
/db_xref="SGD:S000005019"
/translation="MLRRQARERREYLYRKAQELQDSQLQQKRQIIKQALAQGKPLPK
ELAEDESLQKDFRYDQSLKESEEADDLQVDDEYAATSGIMDPRIIVTTSRDPSTRLSQ
FAKEIKLLFPNAVRLNRGNYVMPNLVDACKKSGTTDLVVLHEHRGVPTSLTISHFPHG
PTAQFSLHNVVMRHDIINAGNQSEVNPHLIFDNFTTALGKRVVCILKHLFNAGPKKDS
ERVITFANRGDFISVRQHVYVRTREGVEIAEVGPRFEMRLFELRLGTLENKDADVEWQ
LRRFIRTANKKDYL"
gene complement(<486766..>488124)
/gene="MLF3"
/locus_tag="YNL074C"
/gene_synonym="YMK1"
/db_xref="GeneID:855650"
mRNA complement(<486766..>488124)
/gene="MLF3"
/locus_tag="YNL074C"
/gene_synonym="YMK1"
/product="Mlf3p"
/transcript_id="NM_001182912.3"
/db_xref="GeneID:855650"
CDS complement(486766..488124)
/gene="MLF3"
/locus_tag="YNL074C"
/gene_synonym="YMK1"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:16816427]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0030950
establishment or maintenance of actin cytoskeleton
polarity [PMID:16816427]"
/experiment="EXISTENCE:genetic interaction:GO:0034605
cellular response to heat [PMID:16816427]"
/note="Serine-rich hypothetical protein; predicted to be
palmitoylated; overproduction suppresses growth inhibition
caused by exposure to immunosuppressant leflunomide; MLF3
has a paralog, VHS2, that arose from the whole genome
duplication"
/codon_start=1
/product="Mlf3p"
/protein_id="NP_014325.3"
/db_xref="GeneID:855650"
/db_xref="SGD:S000005018"
/translation="MCVYKSNSNNSNPSFIFERTVQEASSNDLFLQPPVSASNTSHSS
RSNSFYNLQTISPIPISGSEVRTPSLRKNSNNVSSPLDNVIPTSRSASNSTTSSLAHQ
EYILNPICNMQNHHHRRRTLENSVAPALDASCSIVNDENTDLSDVDMVYSRRPSSAVS
LNMALLARTNSATLPSSESSPASPDLKLSRSHSHSAATRPTLNNINNTGMTTTTSNGE
PNSRILRFYSYVDMLNDEKLAQANNTPTSRPPMKSQAYSCPFILKRSPPQAYSSSSAT
TTFSNPFIKTTELPATSPYVSPQQSARQYSNNANNNAKSPKNRSSSILFQRQSILSNV
DPVANMHKNPKFQIESSDSEEEDLTMDMLDPSFPLSSSLRSSANLASNPELATQTPLS
TSSSYTAIGKPMPLSTDPSYVSSSNTLSSEHELRVEKVSEVLKKKVSNGGFSTEFNSC
DT"
gene <488386..>490116
/gene="MSK1"
/locus_tag="YNL073W"
/db_xref="GeneID:855651"
mRNA <488386..>490116
/gene="MSK1"
/locus_tag="YNL073W"
/product="lysine--tRNA ligase MSK1"
/transcript_id="NM_001182911.1"
/db_xref="GeneID:855651"
CDS 488386..490116
/gene="MSK1"
/locus_tag="YNL073W"
/EC_number="6.1.1.6"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:16823961|PMID:14576278|PMID:24769239|PMID:2016746]"
/experiment="EXISTENCE:direct assay:GO:0008033 tRNA
processing [PMID:17560369]"
/experiment="EXISTENCE:mutant phenotype:GO:0004824
lysine-tRNA ligase activity [PMID:2016746]"
/experiment="EXISTENCE:mutant phenotype:GO:0032543
mitochondrial translation [PMID:17560369]"
/experiment="EXISTENCE:mutant phenotype:GO:0070154
mitochondrial lysyl-tRNA aminoacylation [PMID:2016746]"
/note="Mitochondrial lysine-tRNA synthetase; required for
import of both aminoacylated and deacylated forms of
tRNA(Lys) into mitochondria and for aminoacylation of
mitochondrially encoded tRNA(Lys)"
/codon_start=1
/product="lysine--tRNA ligase MSK1"
/protein_id="NP_014326.1"
/db_xref="GeneID:855651"
/db_xref="SGD:S000005017"
/translation="MNVLLKRRSLTFAPRWLWCKCRSSRSRPYSLAHAVDTSKMEATR
RNGQIVKDLGRYYPSMSESALHDLCQEYKEVTIADFNERFLGNPATLHHEDNPNLLLS
INGRIKSIRFSGQKIVFIDLYNGSSGLKNDTQLQLIVNYNKIGGSSEDKANFSEYMNF
LKKGDYIKALGYPGFSQSRVKMLSLICNKLPIVLSVSQLPLPSRLNDETKIKSNRVVD
YQLNGTQTLLVRARIIKLLRKFLDDRNFVEVETPILSSKSNGAMAKPFITSSKDFDHL
ELRIAPELWLKRLIISGLQKVYEIGKVFRNEGIDSTHNAEFSTLEFYETYMSMDDIVT
RTEDLFKFLITNLQKFFQDTRLPVPKTFSELHLALSENNWKFRKVEFLPTLNKELGID
LMNSGLDINKPSELLKALPKDIAKKYFPSADNTGQLSSLQILNKLSDVFLEQRHCQST
LPTVIYHQPAILSPLAKTDPQNKQVTKRFEVFIKGKEYINAYEEENCPQLQLQKFLQQ
KQINELTGNKTETLSPVIDYQYVETMKYGMPPVGGFGLGIDRLCMLFCDKKRIEEVLP
FGCVDDVNRQ"
gene <490317..>491240
/gene="RNH201"
/locus_tag="YNL072W"
/gene_synonym="RNH35"
/db_xref="GeneID:855652"
mRNA <490317..>491240
/gene="RNH201"
/locus_tag="YNL072W"
/gene_synonym="RNH35"
/product="ribonuclease H2 catalytic subunit RNH201"
/transcript_id="NM_001182910.1"
/db_xref="GeneID:855652"
CDS 490317..491240
/gene="RNH201"
/locus_tag="YNL072W"
/gene_synonym="RNH35"
/EC_number="3.1.26.4"
/experiment="EXISTENCE:direct assay:GO:0004523 RNA-DNA
hybrid ribonuclease activity [PMID:14734815]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11029655]"
/experiment="EXISTENCE:direct assay:GO:0032299
ribonuclease H2 complex [PMID:14734815]"
/experiment="EXISTENCE:direct assay:GO:0043137 DNA
replication, removal of RNA primer [PMID:10567561]"
/experiment="EXISTENCE:genetic interaction:GO:0043137 DNA
replication, removal of RNA primer [PMID:10567561]"
/experiment="EXISTENCE:mutant phenotype:GO:0004523 RNA-DNA
hybrid ribonuclease activity [PMID:14734815]"
/note="Ribonuclease H2 catalytic subunit; removes RNA
primers during Okazaki fragment synthesis and errant
ribonucleotides misincorporated during DNA replication;
role in ribonucleotide excision repair; homolog of RNAse
HI; related to human AGS4 which causes Aicardi-Goutieres
syndrome"
/codon_start=1
/product="ribonuclease H2 catalytic subunit RNH201"
/protein_id="NP_014327.1"
/db_xref="GeneID:855652"
/db_xref="SGD:S000005016"
/translation="MVPPTVEASLESPYTKSYFSPVPSALLEQNDSPIIMGIDEAGRG
PVLGPMVYAVAYSTQKYQDETIIPNYEFDDSKKLTDPIRRMLFSKIYQDNEELTQIGY
ATTCITPLDISRGMSKFPPTRNYNLNEQAHDVTMALIDGVIKQNVKLSHVYVDTVGPP
ASYQKKLEQRFPGVKFTVAKKADSLYCMVSVASVVAKVTRDILVESLKRDPDEILGSG
YPSDPKTVAWLKRNQTSLMGWPANMVRFSWQTCQTLLDDASKNSIPIKWEEQYMDSRK
NAAQKTKQLQLQMVAKPVRRKRLRTLDNWYR"
gene <491523..>492971
/gene="LAT1"
/locus_tag="YNL071W"
/gene_synonym="ODP2; PDA2"
/db_xref="GeneID:855653"
mRNA <491523..>492971
/gene="LAT1"
/locus_tag="YNL071W"
/gene_synonym="ODP2; PDA2"
/product="dihydrolipoyllysine-residue acetyltransferase"
/transcript_id="NM_001182909.3"
/db_xref="GeneID:855653"
CDS 491523..492971
/gene="LAT1"
/locus_tag="YNL071W"
/gene_synonym="ODP2; PDA2"
/EC_number="2.3.1.12"
/experiment="EXISTENCE:direct assay:GO:0004742
dihydrolipoyllysine-residue acetyltransferase activity
[PMID:2271545]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:14576278|PMID:24769239|PMID:14562095|PMID:11914276|P
MID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0006086 pyruvate
decarboxylation to acetyl-CoA [PMID:7947791]"
/experiment="EXISTENCE:direct assay:GO:0045254 pyruvate
dehydrogenase complex [PMID:2007123|PMID:9038189]"
/note="Dihydrolipoamide acetyltransferase component (E2)
of the PDC; the pyruvate dehydrogenase complex (PDC)
catalyzes the oxidative decarboxylation of pyruvate to
acetyl-CoA; metabolic longevity factor required for
calorie restriction-mediated life span extension"
/codon_start=1
/product="dihydrolipoyllysine-residue acetyltransferase"
/protein_id="NP_014328.3"
/db_xref="GeneID:855653"
/db_xref="SGD:S000005015"
/translation="MSAFVRVVPRISRSSVLTRSLRLQLRCYASYPEHTIIGMPALSP
TMTQGNLAAWTKKEGDQLSPGEVIAEIETDKAQMDFEFQEDGYLAKILVPEGTKDIPV
NKPIAVYVEDKADVPAFKDFKLEDSGSDSKTSTKAQPAEPQAEKKQEAPAEETKTSAP
EAKKSDVAAPQGRIFASPLAKTIALEKGISLKDVHGTGPRGRITKADIESYLEKSSKQ
SSQTSGAAAATPAAATSSTTAGSAPSPSSTASYEDVPISTMRSIIGERLLQSTQGIPS
YIVSSKISISKLLKLRQSLNATANDKYKLSINDLLVKAITVAAKRVPDANAYWLPNEN
VIRKFKNVDVSVAVATPTGLLTPIVKNCEAKGLSQISNEIKELVKRARINKLAPEEFQ
GGTICISNMGMNNAVNMFTSIINPPQSTILAIATVERVAVEDAAAENGFSFDNQVTIT
GTFDHRTIDGAKGAEFMKELKTVIENPLEMLL"
gene <493366..>493548
/gene="TOM7"
/locus_tag="YNL070W"
/gene_synonym="MOM7; YOK22"
/db_xref="GeneID:855654"
mRNA <493366..>493548
/gene="TOM7"
/locus_tag="YNL070W"
/gene_synonym="MOM7; YOK22"
/product="Tom7p"
/transcript_id="NM_001182908.1"
/db_xref="GeneID:855654"
CDS 493366..493548
/gene="TOM7"
/locus_tag="YNL070W"
/gene_synonym="MOM7; YOK22"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:16407407]"
/experiment="EXISTENCE:direct assay:GO:0005742
mitochondrial outer membrane translocase complex
[PMID:9774667]"
/experiment="EXISTENCE:mutant phenotype:GO:0005742
mitochondrial outer membrane translocase complex
[PMID:8641278]"
/experiment="EXISTENCE:mutant phenotype:GO:0008320 protein
transmembrane transporter activity [PMID:8641278]"
/experiment="EXISTENCE:mutant phenotype:GO:0030150 protein
import into mitochondrial matrix [PMID:8641278]"
/experiment="EXISTENCE:mutant phenotype:GO:0045040 protein
insertion into mitochondrial outer membrane
[PMID:8641278]"
/note="Component of the TOM (translocase of outer
membrane) complex; responsible for recognition and initial
import steps for all mitochondrially directed proteins;
promotes assembly and stability of the TOM complex"
/codon_start=1
/product="Tom7p"
/protein_id="NP_014329.1"
/db_xref="GeneID:855654"
/db_xref="SGD:S000005014"
/translation="MSFLPSFILSDESKERISKILTLTHNVAHYGWIPFVLYLGWAHT
SNRPNFLNLLSPLPSV"
gene complement(<493956..>495001)
/gene="RPL16B"
/locus_tag="YNL069C"
/gene_synonym="RP23"
/db_xref="GeneID:855655"
mRNA complement(join(<493956..494524,494974..>495001))
/gene="RPL16B"
/locus_tag="YNL069C"
/gene_synonym="RP23"
/product="60S ribosomal protein uL13 RPL16B"
/transcript_id="NM_001182907.1"
/db_xref="GeneID:855655"
CDS complement(join(493956..494524,494974..495001))
/gene="RPL16B"
/locus_tag="YNL069C"
/gene_synonym="RP23"
/experiment="EXISTENCE:curator inference:GO:0002181
cytoplasmic translation [PMID:11983894]"
/experiment="EXISTENCE:curator inference:GO:0003735
structural constituent of ribosome [PMID:11983894]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:6337137]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:27374275]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:11983894]"
/experiment="EXISTENCE:mutant phenotype:GO:0000470
maturation of LSU-rRNA [PMID:27374275]"
/note="Ribosomal 60S subunit protein L16B; N-terminally
acetylated, binds 5.8 S rRNA; transcriptionally regulated
by Rap1p; homologous to mammalian ribosomal protein L13A
and bacterial L13; RPL16B has a paralog, RPL16A, that
arose from the whole genome duplication"
/codon_start=1
/product="60S ribosomal protein uL13 RPL16B"
/protein_id="NP_014330.1"
/db_xref="GeneID:855655"
/db_xref="SGD:S000005013"
/translation="MSQPVVVIDAKDHLLGRLASTIAKQVLNGQKIVVVRAEALNISG
EFFRNKLKYHDFLRKATAFNKTRGPFHFRAPSRILYKAIRGMVSHKTARGKAALERLK
IFEGIPPPYDKKKRVVVPQALRVLRLKPGRKYTTLGKLSTSVGWKYEDVVAKLEDKRK
VRSAEYYAKKRAFTKKVSSASAAASESDVAKQLASFGY"
gene complement(<495701..>498289)
/gene="FKH2"
/locus_tag="YNL068C"
/db_xref="GeneID:855656"
mRNA complement(<495701..>498289)
/gene="FKH2"
/locus_tag="YNL068C"
/product="forkhead family transcription factor FKH2"
/transcript_id="NM_001182906.3"
/db_xref="GeneID:855656"
CDS complement(495701..498289)
/gene="FKH2"
/locus_tag="YNL068C"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:24504085]"
/experiment="EXISTENCE:direct assay:GO:0003688 DNA
replication origin binding [PMID:24504085|PMID:22265405]"
/experiment="EXISTENCE:direct assay:GO:0003712
transcription coregulator activity
[PMID:10959837|PMID:15509804]"
/experiment="EXISTENCE:direct assay:GO:0003713
transcription coactivator activity [PMID:10899128]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476|PMID:10899128|PMID:20847055]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding
[PMID:24504085|PMID:19111667]"
/experiment="EXISTENCE:direct assay:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding
[PMID:10899128|PMID:15509804|PMID:12711672|PMID:10959837]"
/experiment="EXISTENCE:genetic interaction:GO:0000082 G1/S
transition of mitotic cell cycle [PMID:17898805]"
/experiment="EXISTENCE:genetic interaction:GO:0000086 G2/M
transition of mitotic cell cycle
[PMID:11562353|PMID:10747051|PMID:10894549|PMID:10894548|P
MID:15509804|PMID:10959837]"
/experiment="EXISTENCE:genetic interaction:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:17898805|PMID:10894549]"
/experiment="EXISTENCE:genetic interaction:GO:0006338
chromatin remodeling [PMID:17283050]"
/experiment="EXISTENCE:genetic interaction:GO:0032968
positive regulation of transcription elongation by RNA
polymerase II [PMID:12702877]"
/experiment="EXISTENCE:genetic interaction:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:11562353|PMID:15509804|PMID:10959837|PMID:10894548|P
MID:10747051]"
/experiment="EXISTENCE:genetic interaction:GO:0061186
negative regulation of silent mating-type cassette
heterochromatin formation [PMID:10747051]"
/experiment="EXISTENCE:genetic interaction:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:10959837|PMID:15509804]"
/experiment="EXISTENCE:genetic interaction:GO:2000221
negative regulation of pseudohyphal growth
[PMID:10747051|PMID:10894548]"
/experiment="EXISTENCE:mutant phenotype:GO:0000086 G2/M
transition of mitotic cell cycle
[PMID:10959837|PMID:10899128]"
/experiment="EXISTENCE:mutant phenotype:GO:0003712
transcription coregulator activity [PMID:15509804]"
/experiment="EXISTENCE:mutant phenotype:GO:0006338
chromatin remodeling [PMID:17283050]"
/experiment="EXISTENCE:mutant phenotype:GO:0032298
positive regulation of DNA-templated DNA replication
initiation [PMID:22265405]"
/experiment="EXISTENCE:mutant phenotype:GO:0032968
positive regulation of transcription elongation by RNA
polymerase II [PMID:12702877]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:10899128|PMID:10959837]"
/experiment="EXISTENCE:mutant phenotype:GO:0061186
negative regulation of silent mating-type cassette
heterochromatin formation [PMID:10747051]"
/experiment="EXISTENCE:mutant phenotype:GO:0061629 RNA
polymerase II-specific DNA-binding transcription factor
binding [PMID:12711672|PMID:15509804|PMID:10899128]"
/experiment="EXISTENCE:mutant phenotype:GO:1903468
positive regulation of DNA replication initiation
[PMID:26728715]"
/experiment="EXISTENCE:mutant phenotype:GO:2000221
negative regulation of pseudohyphal growth
[PMID:10894548]"
/note="Forkhead family transcription factor; rate-limiting
activator of replication origins; evolutionarily conserved
regulator of lifespan; binds multiple chromosomal elements
with distinct specificities, cell cycle dynamics;
positively regulates transcriptional elongation;
facilitates clustering, activation of early-firing
replication origins; negative role in chromatin silencing
at HML and HMR; major role in expression of G2/M phase
genes; relocalizes to cytosol under hypoxia"
/codon_start=1
/product="forkhead family transcription factor FKH2"
/protein_id="NP_014331.3"
/db_xref="GeneID:855656"
/db_xref="SGD:S000005012"
/translation="MSSSNFNEMNELNMTQTNYGSTKYTAQHHQGVINAIISSLTAPD
QPTTVSLQYSNDKNMATEIQAYAKLSGPNWTYYVKDLEVSIGRNTDPLNSALQENSDG
VKNSYRVNIDLGPAKVVSRKHAIIKYNMNIGGWELHILGRNGAKVNFQRTHNGPNNPP
IRLSSGTLLDIGGTQMMFILPDSDPVVAPICIEHLMPNLINMFGLEGNNNPLLRDIIK
QSNYAKQRQLTSNQQIKGFKLYGSGGNAPFGSGANLGPSEQGIFNNNNNSKNKNGYFT
SINPNYTASTTTSNTINPQAASPQGPPNTIIAANFVDSYKSSNAYPQALDFTSDLSHD
ENRNVKPPHSYATMITQAILSSPEGVISLADIYKYISSNYAYYRFAKSGWQNSIRHNL
SLNKAFEKVPRRPNEPGKGMKWRISESYQQEFLNKWNTGKVGKIRRGSSVARQLQLHM
AKFNSLPMEMDYRLSLNMAQPPKRQLQSHNVLEPSNNNIIEGFVQHVPSKGNLPASQQ
SQPPVSHQNQSQQPPPQEQRQEIQFTFADTQNRNIALARPIKTPQLQAPNSNANLNQN
NMKEYKESLHPPAISISQMNRQSPNNALVSFTNACANSKIINNISDSADKSTNNNGGT
KMNLPAISTSSLDENGNLEPTTTTSSGNSNSVPQTGTTTSSLAANSLRLSQPYDTLLR
SPTKAFHITAMEAYTPERGSANRARSPLHSNSNNTNNNGANNSNLQTSGMENKQTGLV
LDSNVLKSMESNNDNRRLTPSTSKSQNVKSSPGVWNLLQFSSTNNTPAADSGGNKRGF
SINPDIKAKENENATSEKDSDSNSNDLETKDINSSPLKNQGGSTANAKELILDTDGAK
ISIINN"
rep_origin 498970..499154
/note="ARS1420; Autonomously Replicating Sequence"
/db_xref="SGD:S000118388"
gene <499417..>499557
/locus_tag="YNL067W-B"
/gene_synonym="YNL067W-A"
/db_xref="GeneID:1466514"
mRNA <499417..>499557
/locus_tag="YNL067W-B"
/gene_synonym="YNL067W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184639.3"
/db_xref="GeneID:1466514"
CDS 499417..499557
/locus_tag="YNL067W-B"
/gene_synonym="YNL067W-A"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878156.3"
/db_xref="GeneID:1466514"
/db_xref="SGD:S000028810"
/translation="MCKLMWCTGVVSKTALLTGNFFFSSSEFFFKATHRKSENYLNGR
QT"
gene <499681..>500256
/gene="RPL9B"
/locus_tag="YNL067W"
/db_xref="GeneID:855658"
mRNA <499681..>500256
/gene="RPL9B"
/locus_tag="YNL067W"
/product="60S ribosomal protein uL6 RPL9B"
/transcript_id="NM_001182905.1"
/db_xref="GeneID:855658"
CDS 499681..500256
/gene="RPL9B"
/locus_tag="YNL067W"
/experiment="EXISTENCE:direct assay:GO:0002181 cytoplasmic
translation [PMID:18782943]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:18782943]"
/experiment="EXISTENCE:direct assay:GO:0022625 cytosolic
large ribosomal subunit [PMID:18782943]"
/note="Ribosomal 60S subunit protein L9B; homologous to
mammalian ribosomal protein L9 and bacterial L6; RPL9B has
a paralog, RPL9A, that arose from a single-locus
duplication"
/codon_start=1
/product="60S ribosomal protein uL6 RPL9B"
/protein_id="NP_014332.1"
/db_xref="GeneID:855658"
/db_xref="SGD:S000005011"
/translation="MKYIQTEQQIEIPEGVTVSIKSRIVKVVGPRGTLTKNLKHIDVT
FTKVNNQLIKVAVHNGDRKHVAALRTVKSLVDNMITGVTKGYKYKMRYVYAHFPINVN
IVEKDGAKFIEVRNFLGDKKIRNVPVRDGVTIEFSTNVKDEIVLSGNSVEDVSQNAAD
LQQICRVRNKDIRKFLDGIYVSHKGFIVEDM"
gene <501515..>502777
/gene="SUN4"
/locus_tag="YNL066W"
/gene_synonym="SCW3"
/db_xref="GeneID:855659"
mRNA <501515..>502777
/gene="SUN4"
/locus_tag="YNL066W"
/gene_synonym="SCW3"
/product="putative glucosidase SUN4"
/transcript_id="NM_001182904.1"
/db_xref="GeneID:855659"
CDS 501515..502777
/gene="SUN4"
/locus_tag="YNL066W"
/gene_synonym="SCW3"
/experiment="EXISTENCE:direct assay:GO:0005621 cellular
bud scar [PMID:27229769]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:11958935]"
/note="Cell wall protein related to glucanases localized
in birth scars; possibly involved in cell wall septation;
member of the SUN family; SUN4 has a paralog, SIM1, that
arose from the whole genome duplication"
/codon_start=1
/product="putative glucosidase SUN4"
/protein_id="NP_014333.1"
/db_xref="GeneID:855659"
/db_xref="SGD:S000005010"
/translation="MKLSATTLTAASLIGYSTIVSALPYAADIDTGCTTTAHGSHQHK
RAVAVTYVYETVTVDKNGQTVTPTSTEASSTVASTTTLISESSVTKSSSKVASSSEST
EQIATTSSSAQTTLTSSETSTSESSVPISTSGSASTSSAASSATGSIYGDLADFSGPY
EKFEDGTIPCGQFPSGQGVIPISWLDEGGWSGVENTDTSTGGSCKEGSYCSYACQPGM
SKTQWPSDQPSDGRSIGGLLCKDGYLYRSNTDTDYLCEWGVDAAYVVSELSNDVAICR
TDYPGTENMVIPTYVQAGDSLPLTVVDQDTYYTWQGLKTSAQYYVNNAGISVEDACVW
GSSSSGVGNWAPLNFGAGSSDGVAYLSLIPNPNNGNALNFNVKIVAADDSSTVNGECI
YENGSFSGGSDGCTVSVTAGKAKFVLYN"
gene <503724..>505484
/gene="AQR1"
/locus_tag="YNL065W"
/db_xref="GeneID:855660"
mRNA <503724..>505484
/gene="AQR1"
/locus_tag="YNL065W"
/product="Aqr1p"
/transcript_id="NM_001182903.3"
/db_xref="GeneID:855660"
CDS 503724..505484
/gene="AQR1"
/locus_tag="YNL065W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:34887841|PMID:11922628|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0032973
amino acid export across plasma membrane [PMID:15590823]"
/experiment="EXISTENCE:mutant phenotype:GO:0008028
monocarboxylic acid transmembrane transporter activity
[PMID:11922628]"
/experiment="EXISTENCE:mutant phenotype:GO:0015565
threonine efflux transmembrane transporter activity
[PMID:34887841]"
/experiment="EXISTENCE:mutant phenotype:GO:0015718
monocarboxylic acid transport [PMID:11922628]"
/experiment="EXISTENCE:mutant phenotype:GO:0032973 amino
acid export across plasma membrane
[PMID:34887841|PMID:15590823]"
/experiment="EXISTENCE:mutant phenotype:GO:0042910
xenobiotic transmembrane transporter activity
[PMID:11922628]"
/experiment="EXISTENCE:mutant phenotype:GO:0055085
transmembrane transport [PMID:11922628]"
/note="Plasma membrane transporter of the major
facilitator superfamily; involved in the excretion of
excess amino acids; member of the 12-spanner drug:H(+)
antiporter DHA1 family; confers resistance to short-chain
monocarboxylic acids and quinidine; relocalizes from
plasma membrane to cytoplasm upon DNA replication stress;
AQR1 has a paralog, QDR1, that arose from the whole genome
duplication"
/codon_start=1
/product="Aqr1p"
/protein_id="NP_014334.3"
/db_xref="GeneID:855660"
/db_xref="SGD:S000005009"
/translation="MSRSNSIYTEDIEMYPTHNEQHLTREYTKPDGQTKSEKLNFEGA
YINSHGTLSKTTTREIEGDLDSETSSHSSDDKVDPTQQITAETKAPYTLLSYGQKWGM
VAILTMCGFWSSLGSPIYYPALRQLEKQFNVDENMVNVTVVVYLLFQGISPTVSGGLA
DCFGRRPIILAGMLIYVIASIGLACAPSYGVIIFLRCIQSIGISPTIAISSGVVGDFT
LKHERGTFVGATSGFVLLGQCFGSLIGAVLTARWDWRAIFWFLTIGCGSCFLIAFLIL
PETKRTIAGNLSIKPKRFINRAPIFLLGPVRRRFKYDNPDYETLDPTIPKLDLSSAGK
ILVLPEIILSLFPSGLLFAMWTLMLSSISSGLSVAPYNYHLVIIGVCYLPGGIGGLMG
SFFTGRIIDMYFKRKIKKFEQDKANGLIPQDAEINMFKVRLVCLLPQNFLAVVAYLLF
GWSIDKGWRIESILITSFVCSYCAMSTLSTSTTLLVDLYPTKSSTASSCFNFVRCSLS
TIFMGCFAKMKAAMTVGGTFTFLCALVFFFNFLMFIPMKYGMKWREDRLLKQQRQSWL
NTLAVKAKKGTKRDQNDNHN"
gene complement(<505868..>507097)
/gene="YDJ1"
/locus_tag="YNL064C"
/gene_synonym="HSP40; MAB3; MAS5"
/db_xref="GeneID:855661"
mRNA complement(<505868..>507097)
/gene="YDJ1"
/locus_tag="YNL064C"
/gene_synonym="HSP40; MAB3; MAS5"
/product="type I HSP40 co-chaperone YDJ1"
/transcript_id="NM_001182902.1"
/db_xref="GeneID:855661"
CDS complement(505868..507097)
/gene="YDJ1"
/locus_tag="YNL064C"
/gene_synonym="HSP40; MAB3; MAS5"
/experiment="EXISTENCE:direct assay:GO:0001671 ATPase
activator activity [PMID:15342786|PMID:1400408]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:1869583|PMID:26928762|PMID:8144572]"
/experiment="EXISTENCE:direct assay:GO:0042026 protein
refolding [PMID:9674429]"
/experiment="EXISTENCE:direct assay:GO:0048471 perinuclear
region of cytoplasm [PMID:1869583]"
/experiment="EXISTENCE:direct assay:GO:0051082 unfolded
protein binding [PMID:9774392]"
/experiment="EXISTENCE:direct assay:GO:0072380 TRC complex
[PMID:20850366]"
/experiment="EXISTENCE:mutant phenotype:GO:0006458 'de
novo' protein folding [PMID:10567418]"
/experiment="EXISTENCE:mutant phenotype:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:25344756]"
/experiment="EXISTENCE:mutant phenotype:GO:0006626 protein
targeting to mitochondrion [PMID:1729605]"
/experiment="EXISTENCE:mutant phenotype:GO:0009267
cellular response to starvation [PMID:25853343]"
/experiment="EXISTENCE:mutant phenotype:GO:0034605
cellular response to heat [PMID:25344756]"
/experiment="EXISTENCE:mutant phenotype:GO:0035719 tRNA
import into nucleus [PMID:25853343]"
/experiment="EXISTENCE:mutant phenotype:GO:0036503 ERAD
pathway [PMID:15342786|PMID:15252059]"
/experiment="EXISTENCE:mutant phenotype:GO:0045047 protein
targeting to ER [PMID:1473150]"
/note="Type I HSP40 co-chaperone; regulates HSP90 and
HSP70 functions; acts as an adaptor that helps Rsp5p
recognize and ubiquitinate misfolded, cytosolic proteins
after heat shock; critical for determining cell size at
Start as a function of growth rate; involved in protein
translocation across membranes; forms cytotoxic
amyloidogenic-like structures in vitro; DnaJ family
member; chimeric protein in which the human p58IPK J
domain replaces the yeast Ydj1p J domain can complement
yeast ydj1 mutant"
/codon_start=1
/product="type I HSP40 co-chaperone YDJ1"
/protein_id="NP_014335.1"
/db_xref="GeneID:855661"
/db_xref="SGD:S000005008"
/translation="MVKETKFYDILGVPVTATDVEIKKAYRKCALKYHPDKNPSEEAA
EKFKEASAAYEILSDPEKRDIYDQFGEDGLSGAGGAGGFPGGGFGFGDDIFSQFFGAG
GAQRPRGPQRGKDIKHEISASLEELYKGRTAKLALNKQILCKECEGRGGKKGAVKKCT
SCNGQGIKFVTRQMGPMIQRFQTECDVCHGTGDIIDPKDRCKSCNGKKVENERKILEV
HVEPGMKDGQRIVFKGEADQAPDVIPGDVVFIVSERPHKSFKRDGDDLVYEAEIDLLT
AIAGGEFALEHVSGDWLKVGIVPGEVIAPGMRKVIEGKGMPIPKYGGYGNLIIKFTIK
FPENHFTSEENLKKLEEILPPRIVPAIPKKATVDECVLADFDPAKYNRTRASRGGANY
DSDEEEQGGEGVQCASQ"
gene <507758..>508702
/gene="MTQ1"
/locus_tag="YNL063W"
/db_xref="GeneID:855662"
mRNA <507758..>508702
/gene="MTQ1"
/locus_tag="YNL063W"
/product="S-adenosylmethionine-dependent
methyltransferase"
/transcript_id="NM_001182901.1"
/db_xref="GeneID:855662"
CDS 507758..508702
/gene="MTQ1"
/locus_tag="YNL063W"
/EC_number="2.1.1.297"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008757
S-adenosylmethionine-dependent methyltransferase activity
[PMID:16321977]"
/experiment="EXISTENCE:genetic interaction:GO:0006451
translational readthrough [PMID:16321977]"
/experiment="EXISTENCE:mutant phenotype:GO:0008757
S-adenosylmethionine-dependent methyltransferase activity
[PMID:16321977]"
/note="S-adenosylmethionine-dependent methyltransferase;
methylates translational release factor Mrf1p; similar to
E.coli PrmC; is not an essential gene"
/codon_start=1
/product="S-adenosylmethionine-dependent
methyltransferase"
/protein_id="NP_014336.1"
/db_xref="GeneID:855662"
/db_xref="SGD:S000005007"
/translation="MPRISTSLIRKASRIRPGLHLLLPECRTLEQAKLEYKWLTEELP
PDKSIRWACLQRYKHVPLQYILRSQPFGALDIVCKPGVLIPRWETEEWVMAIIRALNN
SMLSRHTIPLHICDTFTGTGCIALALSHGIANCTFTAIDVSTRAIKLVKENMLKNKVS
GGKLVQHNILSSKASDEYPSHIDILTGNPPYIRKRDFNRDVKTSVKLFEPRLALVGEL
ECYINLVNYWLPKTDSFFYEIGDVEQFNYVERRIKEDSYLSRIWSIGLKYDSNGKARV
VYGFKATPKGRILHQIFASFGTIRHLATALSGHKANCN"
gene complement(<508776..>510212)
/gene="GCD10"
/locus_tag="YNL062C"
/gene_synonym="TRM6"
/db_xref="GeneID:855663"
mRNA complement(<508776..>510212)
/gene="GCD10"
/locus_tag="YNL062C"
/gene_synonym="TRM6"
/product="tRNA 1-methyladenosine methyltransferase subunit
GCD10"
/transcript_id="NM_001182900.3"
/db_xref="GeneID:855663"
CDS complement(508776..510212)
/gene="GCD10"
/locus_tag="YNL062C"
/gene_synonym="TRM6"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9851972]"
/experiment="EXISTENCE:direct assay:GO:0030488 tRNA
methylation [PMID:10779558]"
/experiment="EXISTENCE:direct assay:GO:0031515 tRNA (m1A)
methyltransferase complex [PMID:10779558]"
/experiment="EXISTENCE:direct assay:GO:0160107 tRNA
(adenine(58)-N1)-methyltransferase activity
[PMID:10779558]"
/experiment="EXISTENCE:physical interaction:GO:0031515
tRNA (m1A) methyltransferase complex [PMID:10779558]"
/note="Subunit of tRNA (1-methyladenosine)
methyltransferase with Gcd14p; required for the
modification of the adenine at position 58 in tRNAs,
especially tRNAi-Met; first identified as a negative
regulator of GCN4 expression"
/codon_start=1
/product="tRNA 1-methyladenosine methyltransferase subunit
GCD10"
/protein_id="NP_014337.3"
/db_xref="GeneID:855663"
/db_xref="SGD:S000005006"
/translation="MNALTTIDFNQHVIVRLPSKNYKIVELKPNTSVSLGKFGAFEVN
DIIGYPFGLTFEIYYDGEEVSSDENRDSKPKNKIPIGKVRLLSQEIKDVNNDKDDGQS
EPPLSIKEKSVSLELSSIDSSATNQNLVNMGSKAQELTVEEIEKMKQESLSSKEIIDK
IIKSHKSFHNKTVYSQEKYVNRKKQKFAKYFTVEYLSSSNLLQFLIDKGDIQRVLDMS
QESMGMLLNLANIQSEGNYLCMDETGGLLVYFLLERMFGGDNESKSKGKVIVIHENEH
ANLDLLKFANYSEKFIKEHVHTISLLDFFEPPTLQEIQSRFTPLPKEEARALKGGKKN
SYYRKLRWYNTQWQILELTGEFLYDGLVMATTLHLPTLVPKLAEKIHGSRPIVCYGQF
KETLLELAHTLYSDLRFLAPSILETRCRPYQSIRGKLHPLMTMKGGGGYLMWCHRVIP
APEPVSENATAADSSEKLAEHGAKKQKI"
gene <510540..>512396
/gene="NOP2"
/locus_tag="YNL061W"
/gene_synonym="YNA1"
/db_xref="GeneID:855664"
mRNA <510540..>512396
/gene="NOP2"
/locus_tag="YNL061W"
/gene_synonym="YNA1"
/product="rRNA (cytosine-C5-)-methyltransferase NOP2"
/transcript_id="NM_001182899.3"
/db_xref="GeneID:855664"
CDS 510540..512396
/gene="NOP2"
/locus_tag="YNL061W"
/gene_synonym="YNA1"
/EC_number="2.1.1.310"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:26196125|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0009383 rRNA
(cytosine-C5-)-methyltransferase activity [PMID:23913415]"
/experiment="EXISTENCE:direct assay:GO:0030687
preribosome, large subunit precursor
[PMID:17443350|PMID:22735702]"
/experiment="EXISTENCE:direct assay:GO:0070475 rRNA base
methylation [PMID:23913415]"
/experiment="EXISTENCE:genetic interaction:GO:0009383 rRNA
(cytosine-C5-)-methyltransferase activity [PMID:23913415]"
/experiment="EXISTENCE:genetic interaction:GO:0070475 rRNA
base methylation [PMID:23913415]"
/experiment="EXISTENCE:mutant phenotype:GO:0000463
maturation of LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:8972218]"
/experiment="EXISTENCE:mutant phenotype:GO:0009383 rRNA
(cytosine-C5-)-methyltransferase activity [PMID:23913415]"
/experiment="EXISTENCE:mutant phenotype:GO:0042273
ribosomal large subunit biogenesis [PMID:8972218]"
/experiment="EXISTENCE:mutant phenotype:GO:0070475 rRNA
base methylation [PMID:23913415]"
/experiment="EXISTENCE:mutant phenotype:GO:1902626
assembly of large subunit precursor of preribosome
[PMID:22735702]"
/note="rRNA m5C methyltransferase; methylates cytosine at
position 2870 of 25S rRNA; has an essential function
independent of rRNA methylation; contains seven
beta-strand methyltransferase motif; essential for
processing and maturation of 27S pre-rRNA and large
ribosomal subunit biogenesis; localized to the nucleolus;
constituent of 66S pre-ribosomal particles; rRNA
methylation defect and lethality are functionally
complemented by human NOP2, a gene upregulated in cancer"
/codon_start=1
/product="rRNA (cytosine-C5-)-methyltransferase NOP2"
/protein_id="NP_014338.3"
/db_xref="GeneID:855664"
/db_xref="SGD:S000005005"
/translation="MGSRRHKNKQAAPPTLEEFQARKEKKANRKLEKGKRPSTTQGDE
VSDRKKKKSKPFKKSRKEEEEVVEEDKDLPEVDLEELSKARKSLFDDEEDDDEAGLVD
EELKDEFDLEQEYDYDEDEDNDAHPIFSDDDDEADLEELNAQNMEALSKKLDEEEAEE
AEEAEMELVEAENMQPRADILPTEEQEEMMAQETPNLTSTRTRMIEIVKVLENFKTLG
AEGRSRGEYVDRLLKDICEYFGYTPFLAEKLFNLFSPAEAMEFFEANEIARPITIRTN
TLKTRRRDLAQTLVNRGVNLQPIGSWTKVGLQIFDSQVPIGATPEYLAGHYILQAASS
FLPVIALDPHENERILDMAAAPGGKTTYISAMMKNTGCVFANDANKSRTKSLIANIHR
LGCTNTIVCNYDAREFPKVIGGFDRILLDAPCSGTGVIGKDQSVKVSRTEKDFIQIPH
LQKQLLLSAIDSVDCNSKHGGVIVYSTCSVAVEEDEAVIDYALRKRPNVKLVDTGLAI
GKEAFTSYRGKKFHPSVKLARRYYPHTYNVDGFFVAKFQKIGPSSFDDNQASAKEKET
AARKEALEEGIIHSDFATFEDEEDDKYIEKSVKNNLLKKGVNPKAKRPSNEK"
gene complement(<512668..>514935)
/gene="ARP5"
/locus_tag="YNL059C"
/db_xref="GeneID:855665"
mRNA complement(<512668..>514935)
/gene="ARP5"
/locus_tag="YNL059C"
/product="actin-related protein ARP5"
/transcript_id="NM_001182898.1"
/db_xref="GeneID:855665"
CDS complement(512668..514935)
/gene="ARP5"
/locus_tag="YNL059C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11011149|PMID:10923024]"
/experiment="EXISTENCE:direct assay:GO:0006338 chromatin
remodeling [PMID:12887900]"
/experiment="EXISTENCE:direct assay:GO:0030234 enzyme
regulator activity [PMID:26306040]"
/experiment="EXISTENCE:direct assay:GO:0031011 Ino80
complex [PMID:15525518|PMID:12887900]"
/experiment="EXISTENCE:mutant phenotype:GO:0006338
chromatin remodeling [PMID:12887900]"
/experiment="EXISTENCE:mutant phenotype:GO:0031011 Ino80
complex [PMID:12887900]"
/experiment="EXISTENCE:physical interaction:GO:0006338
chromatin remodeling [PMID:15525518]"
/experiment="EXISTENCE:physical interaction:GO:0031011
Ino80 complex [PMID:24034245]"
/note="Nuclear actin-related protein involved in chromatin
remodeling; component of chromatin-remodeling enzyme
complexes; promotes nucleosome shifts in the 3 prime
direction"
/codon_start=1
/product="actin-related protein ARP5"
/protein_id="NP_014339.1"
/db_xref="GeneID:855665"
/db_xref="SGD:S000005004"
/translation="MSSRDASLTPLKAVVIDDPPLRQTPEPFDEQSAYNPQSPIAIDF
GSSKLRAGFVNHATPTHIFPNALTKFRDRKLNKNFTFVGNDTLLDQAVRSQSRSPFDG
PFVTNWNLTEEILDYTFHHLGVVPDNGIPNPILLTERLATVQSQRTNWYQILFETYNV
PGVTFGIDSLFSFYNYNPSGNKTGLVISCGHEDTNVIPVVDGAGILTDAKRINWGGHQ
AVDYLNDLMALKYPYFPTKMSYLQYETMYKDYCYVSRNYDEDIEKILTLENLDTNDVV
VEAPFTEVLQPQKTEEELRIQAEKRKETGKRLQEQARLKRMEKLVQKQEEFEYFSKVR
DQLIDEPKKKVLSVLQNAGFDDERDFKKYLHSLEQSLKKAQMVEAEDDSHLDEMNEDK
TAQKFDLLDIADEDLNEDQIKEKRKQRFLKASQDARQKAKEEKERVAKEEEEKKLKEQ
QWRETDLNGWIKDKRLKLNKLIKRRKEKLKLRDEMKDRKSQVSQNRMKNLASLAEDNV
KQGAKRNRHQATIDNDPNDTFGANDEDWLIYTDITQNPEAFEEALEYEYKDIVELERL
LLEHDPNFTEEDTLEAQYDWRNSILHLFLRGPRPHDSENIHEQHQMHLNVERIRVPEV
IFQPTMGGQDQAGICELSETILLKKFGSQPGKLSQTSIDMVNNVLITGGNAKVPGLKE
RIVKEFTGFLPTGTNITVNMSSDPSLDAWKGMAALARNEEQYRKTVISKKEYEEYGPE
YIKEHKLGNTKYFED"
gene complement(<515763..>516713)
/locus_tag="YNL058C"
/db_xref="GeneID:855667"
mRNA complement(<515763..>516713)
/locus_tag="YNL058C"
/product="uncharacterized protein"
/transcript_id="NM_001182897.1"
/db_xref="GeneID:855667"
CDS complement(515763..516713)
/locus_tag="YNL058C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to vacuole; not an
essential gene; YNL058C has a paralog, PRM5, that arose
from the whole genome duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014340.1"
/db_xref="GeneID:855667"
/db_xref="SGD:S000005003"
/translation="MVKKNFIPSVSLVRRDLPTLVTTTTSSTALSKPTSSVVSETSSK
SLPSLTSSAFSTSSGATSSSSLIVASITPPSTAGNPFILNAADKPNGTVYIAVGAVIG
AIFISILIWWLVSSYLSRRFTMTNSYANDSKNLYRGHHKHSSSLQSNPFDINDEKSYM
QDDWDSMSQLESSQYEDAASPFNPIQDPFTDNRRSLFISPTLQVSQYEKSHSRHQSKD
TNIFIDDPFLYVGTYLEEEEEEEEERKLNLNRPQRAASPERKEKKINSMEGYHKRNQS
SLGLIPVASATSNTSSPKKAHKRQAPSMFLDDVLNGREII"
gene <517249..>517842
/gene="OCA2"
/locus_tag="YNL056W"
/db_xref="GeneID:855668"
mRNA <517249..>517842
/gene="OCA2"
/locus_tag="YNL056W"
/product="Oca2p"
/transcript_id="NM_001182895.3"
/db_xref="GeneID:855668"
CDS 517249..517842
/gene="OCA2"
/locus_tag="YNL056W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="hypothetical protein; similar to predicted tyrosine
phosphatases Oca1p and Siw14p; green fluorescent protein
(GFP)-fusion protein localizes to the cytoplasm; YNL056W
is not an essential gene"
/codon_start=1
/product="Oca2p"
/protein_id="NP_014342.3"
/db_xref="GeneID:855668"
/db_xref="SGD:S000005001"
/translation="MKYIPPLNFSPVVSTDVSLYRSGYPMPLNYSFIKHQLHLKTIIY
IGDKDRPLEEYQSFLESEKIKYYHIFMDSSRDEGIQERMNQVLHLVLDVRNYPILVHS
NKGKHRVGVVVGIIRKLLQGWSTAGICQEYGLFSGGMKDGVDLEFITMFETNLKIPRN
VIPGFAKHCLYLNELEAAEGSDDESGSESILTAKQPI"
gene complement(<517994..>518845)
/gene="POR1"
/locus_tag="YNL055C"
/gene_synonym="OMP2"
/db_xref="GeneID:855669"
mRNA complement(<517994..>518845)
/gene="POR1"
/locus_tag="YNL055C"
/gene_synonym="OMP2"
/product="porin POR1"
/transcript_id="NM_001182894.1"
/db_xref="GeneID:855669"
CDS complement(517994..518845)
/gene="POR1"
/locus_tag="YNL055C"
/gene_synonym="OMP2"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane
[PMID:7499333|PMID:16689936|PMID:16407407]"
/experiment="EXISTENCE:direct assay:GO:0006811 monoatomic
ion transport [PMID:9593723]"
/experiment="EXISTENCE:direct assay:GO:0008308
voltage-gated monoatomic anion channel activity
[PMID:9593723]"
/experiment="EXISTENCE:direct assay:GO:0017128
phospholipid scramblase activity [PMID:38065946]"
/experiment="EXISTENCE:direct assay:GO:0048039 ubiquinone
binding [PMID:38906315|PMID:28051849]"
/experiment="EXISTENCE:mutant phenotype:GO:0005741
mitochondrial outer membrane [PMID:38065946]"
/experiment="EXISTENCE:mutant phenotype:GO:0006915
apoptotic process [PMID:17822411]"
/experiment="EXISTENCE:mutant phenotype:GO:0007005
mitochondrion organization [PMID:11488609]"
/experiment="EXISTENCE:mutant phenotype:GO:0042307
positive regulation of protein import into nucleus
[PMID:29359182]"
/experiment="EXISTENCE:mutant phenotype:GO:0045332
phospholipid translocation [PMID:38065946]"
/experiment="EXISTENCE:mutant phenotype:GO:0045454 cell
redox homeostasis [PMID:18768136]"
/experiment="EXISTENCE:mutant phenotype:GO:0051027 DNA
transport [PMID:19056337]"
/experiment="EXISTENCE:physical interaction:GO:0005739
mitochondrion [PMID:16962558]"
/experiment="EXISTENCE:physical interaction:GO:0044289
mitochondrial inner-outer membrane contact site
[PMID:37073556]"
/note="Mitochondrial porin and phospholipid scramblase;
voltage-dependent anion channel in the outer mitochondrial
membrane required for maintenance of mitochondrial osmotic
stability and membrane permeability; scramblase involved
in mitochondrial transbilayer translocation of
phospholipids; ubiquinone binding protein; couples
glutathione pools of the intermembrane space and the
cytosol; phosphorylated; protein abundance increases in
response to DNA replication stress; orthologous to human
VDAC1 and 2"
/codon_start=1
/product="porin POR1"
/protein_id="NP_014343.1"
/db_xref="GeneID:855669"
/db_xref="SGD:S000005000"
/translation="MSPPVYSDISRNINDLLNKDFYHATPAAFDVQTTTANGIKFSLK
AKQPVKDGPLSTNVEAKLNDKQTGLGLTQGWSNTNNLQTKLEFANLTPGLKNELITSL
TPGVAKSAVLNTTFTQPFFTARGAFDLCLKSPTFVGDLTMAHEGIVGGAEFGYDISAG
SISRYAMALSYFAKDYSLGATLNNEQITTVDFFQNVNAFLQVGAKATMNCKLPNSNVN
IEFATRYLPDASSQVKAKVSDSGIVTLAYKQLLRPGVTLGVGSSFDALKLSEPVHKLG
WSLSFDA"
gene complement(519099..519169)
/locus_tag="YNCN0008C"
/db_xref="GeneID:855670"
tRNA complement(519099..519169)
/locus_tag="YNCN0008C"
/product="tRNA-Asp"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:855670"
/db_xref="SGD:S000006544"
mobile_element 519163..525062
/note="YNLWTy1-2; Ty1 element, LTR retrotransposon of the
Copia (Pseudoviridae) group; contains co-transcribed genes
TYA Gag and TYB Pol, encoding proteins involved in
structure and function of virus-like particles, flanked by
two direct repeats"
/mobile_element_type="retrotransposon:YNLWTy1-2"
/db_xref="SGD:S000007142"
repeat_region 519163..519496
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007136"
gene <519456..>524706
/locus_tag="YNL054W-B"
/db_xref="GeneID:855672"
mRNA <519456..>524706
/locus_tag="YNL054W-B"
/product="gag-pol fusion protein"
/transcript_id="NM_001281536.2"
/db_xref="GeneID:855672"
CDS join(519456..520760,520762..524706)
/locus_tag="YNL054W-B"
/EC_number="2.7.7.7"
/EC_number="2.7.7.49"
/EC_number="3.1.26.4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9448009]"
/ribosomal_slippage
/note="TyB Gag-Pol protein; proteolytically processed to
make the Gag, reverse transcriptase (RT), protease (PR),
and integrase (IN) proteins that are required for
retrotransposition"
/codon_start=1
/product="gag-pol fusion protein"
/protein_id="NP_001268465.1"
/db_xref="GeneID:855672"
/db_xref="SGD:S000007385"
/translation="MESQQLSQHSPISHGSACASVTSKEVHTNQDPLDVSASKIQEYD
KASTKANSQQTTTPASSAVPENPHHASPQTAQSHSPQNGPYPQQCMMTQNQANPSDWS
FYGRPSMIPYTPYQMSPMYFPPGPHSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFLNWVKTYIKFLQNSNLGGIIPTVNGKPVRQITDDELT
FLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQ
YNGSTPADAFETKVTNIIDRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNM
TVAELFLDIHAIYEEQQGSRNSKPNYRRNPSDEKNDSRSYTNTTKPKVIARNPQKTNN
SKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLGQKLTESTVNHTNHS
DDELPGHLLLDSGASRTLIRSAHHIHSASSNPDINVVDAQKRNIPINAIGDLQFHFQD
NTKTSIKVLHTPNIAYDLLSLNELAAVDITACFTKNVLERSDGTVLAPIVKYGDFYWV
SKKYLLPSNISVPTINNVHTSESTRKYPYPFIHRMLAHANAQTIRYSLKNNTITYFNE
SDVDWSSAIDYQCPDCLIGKSTKHRHIKGSRLKYQNSYEPFQYLHTDIFGPVHNLPKS
APSYFISFTDETTKFRWVYPLHDRREDSILDVFTTILAFIKNQFQASVLVIQMDRGSE
YTNRTLHKFLEKNGITPCYTTTADSRAHGVAERLNRTLLDDCRTQLQCSGLPNHLWFS
AIEFSTIVRNSLASPKSKKSARQHAGLAGLDISTLLPFGQPVIVNDHNPNSKIHPRGI
PGYALHPSRNSYGYIIYLPSLKKTVDTTNYVILQGKESRLDQFNYDALTFDEDLNRLT
ASYQSFIASNEIQQSDDLNFQSDIELHPEQPRNVLSKAVSPTDSTPPSTHTEDSKRVS
KTNIRAPREVDPNISKSDILPSKKRSSTPQISDIESTGSGGMHRLDVPLLAPMSQSNT
HESSHTSKSKDFRHSDSYSDNETNHTNVPISSTGGTNNKTVPQTSEQETEKRIIHRSP
SIDTSSSESNSLHHVVPIKTSDTCPKENTEESIIADLPLPDLPPEPPTKLSDSFKELP
PINSRQTNSSLGGIGDSNAYTTINSKKRSLEDNETEIKVSRDTWNTKNMRSLEPPRSK
KRIHLIAAVKAVKSIKPIRTTLRYDEAITYNKDIKEKEKYIEAYHKEVNQLLKMKTWD
TDKYYDRKEIDPKRVINSMFIFNRKRDGTHKARFVARGDIQHPDTYDSGMQSNTVHHY
ALMTSLSLALDNNYYITQLDISSAYLYADIKEELYIRPPPHLGMNDKLIRLKKSLYGL
KQSGANWYETIKSYLIEQCDMEEVRGWSCVFKNSQVTICLFVDDMILFSKDLNANKKI
ITTLKKQYDTKIINLGESDNEIQYDILGLEIKYQRSKYMKLGMEKSLTEKLPKLNVHL
NPKGKKLSAPGQPGLYIDQDELEIDEDEYKEKVHEMQKLIGLASYVGYKFRFDLLYYI
NTLAQHILFPSRQVLDMTYELIQFMWDTRDKQLIWHKNKPTKPDNKLVAISDASYGNQ
PYYKSQIGNIFLLNGKVIGGKSTKASLTCTSTTEAEIHAVSEAIPLLNNLSHLVQELN
KKPIIKGLLTDSRSTISIIKSTNEEKFRNRFFGTKAMRLRDEVSGNNLYVYYIETKKN
IADVMTKPLPIKTFKLLTNKWIH"
gene <519456..>520778
/locus_tag="YNL054W-A"
/db_xref="GeneID:855671"
mRNA <519456..>520778
/locus_tag="YNL054W-A"
/product="gag protein"
/transcript_id="NM_001184413.3"
/db_xref="GeneID:855671"
CDS 519456..520778
/locus_tag="YNL054W-A"
/note="Retrotransposon TYA Gag gene co-transcribed with
TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is
a nucleocapsid protein that is the structural constituent
of virus-like particles (VLPs); similar to retroviral Gag"
/codon_start=1
/product="gag protein"
/protein_id="NP_058180.1"
/db_xref="GeneID:855671"
/db_xref="SGD:S000007384"
/translation="MESQQLSQHSPISHGSACASVTSKEVHTNQDPLDVSASKIQEYD
KASTKANSQQTTTPASSAVPENPHHASPQTAQSHSPQNGPYPQQCMMTQNQANPSDWS
FYGRPSMIPYTPYQMSPMYFPPGPHSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFLNWVKTYIKFLQNSNLGGIIPTVNGKPVRQITDDELT
FLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQ
YNGSTPADAFETKVTNIIDRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNM
TVAELFLDIHAIYEEQQGSRNSKPNYRRNPSDEKNDSRSYTNTTKPKVIARNPQKTNN
SKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLRPETY"
repeat_region 524729..525062
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007137"
gene <526086..>529583
/gene="VAC7"
/locus_tag="YNL054W"
/db_xref="GeneID:855673"
mRNA <526086..>529583
/gene="VAC7"
/locus_tag="YNL054W"
/product="Vac7p"
/transcript_id="NM_001182893.3"
/db_xref="GeneID:855673"
CDS 526086..529583
/gene="VAC7"
/locus_tag="YNL054W"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762|PMID:9372916]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:9372916]"
/experiment="EXISTENCE:direct assay:GO:0070772 PAS complex
[PMID:19037259]"
/experiment="EXISTENCE:genetic interaction:GO:0008047
enzyme activator activity [PMID:11950935]"
/experiment="EXISTENCE:genetic interaction:GO:0010513
positive regulation of phosphatidylinositol biosynthetic
process [PMID:11950935]"
/experiment="EXISTENCE:mutant phenotype:GO:1903778 protein
localization to vacuolar membrane [PMID:17699591]"
/experiment="EXISTENCE:physical interaction:GO:0070772 PAS
complex [PMID:19037259]"
/note="Integral vacuolar membrane protein; involved in
vacuole inheritance and morphology; activates Fab1p kinase
activity under basal conditions and also after
hyperosmotic shock; targeted to vacuole via AP-3 pathway"
/codon_start=1
/product="Vac7p"
/protein_id="NP_014344.3"
/db_xref="GeneID:855673"
/db_xref="SGD:S000004999"
/translation="MTEEDRKLTVETETVEAPVANNLLLSNNSNVVAPNPSIPSASTS
TSPLHREIVDDSVATANTTSNVVQHNLPTIDNNLMDSDATSHNQDHWHSDINRAGTSM
STSDIPTDLHLEHIGSVSSTNNNSNNALINHNPLSSHLSNPSSSLRNKKSSLLVASNP
AFASDVELSKKKPAVISNNMPTSNIALYQTARSANIHGPSSTSASKAFRKASAFSNNT
APSTSNNIGSNTPPAPLLPLPSLSQQNKPKIIERPTMHVTNSREILLGENLLDDTKAK
NAPANSTTHDNGPVANDGLRIPNHSNADDNENNNKMKKNKNINSGKNERNDDTSKICT
TSTKTAPSTAPLGSTDNTQALTASVSSSNADNHNNNKKKTSSNNNGNNSNSASNKTNA
DIKNSNADLSASTSNNNAINDDSHESNSEKPTKADFFAARLATAVGENEISDSEETFV
YESAANSTKNLIFPDSSSQQQQQQQQPPKQQQQQQNHGITSKISAPLLNNNKKLLSRL
KNSRHISTGAILNNTIATISTNPNLNSNVMQNNNNLMSGHNHLDELSSIKQEPPHQLQ
QQQPPMDVQSVDSYTSDNPDSNVIAKSPDKRSSLVSLSKVSPHLLSSTSSNGNTISCP
NVATNSQELEPNNDISTKKSLSNSTLRHSSANRNSNYGDNKRPLRTTVSKIFDSNPNG
APLRRYSGVPDHVNLEDYIEQPHNYPTMQNSVKKDEFYNSRNNKFPHGLNFYGDNNVI
EEENNGDSSNVNRPQHTNLQHEFIPEDNESDENDIHSMFYYNHKNDLETKPLISDYGE
DEDVDDYDRPNATFNSYYGSASNTHELPLHGRMPSRSNNDYYDFMVGNNTGNNNQLNE
YTPLRMKRGQRHLSRTNNSIMNGSIHMNGNDDVTHSNINNNDIVGYSPHNFYSRKSPF
VKVKNFLYLAFVISSLLMTGFILGFLLATNKELQDVDVVVMDNVISSSDELIFDITVS
AFNPGFFSISVSQVDLDIFAKSSYLKCDSNGDCTVMEQERKILQITTNLSLVEESANN
DISGGNIETVLLGTAKKLETPLKFQGGAFNRNYDVSVSSVKLLSPGSREAKHENDDDD
DDDGDDGDDENNTNERQYKSKPNARDDKEDDTKKWKLLIKHDYELIVRGSMKYEVPFF
NTQKSTAIQKDSMVHPGKK"
gene <529942..>531411
/gene="MSG5"
/locus_tag="YNL053W"
/db_xref="GeneID:855674"
mRNA <529942..>531411
/gene="MSG5"
/locus_tag="YNL053W"
/product="tyrosine/serine/threonine protein phosphatase
MSG5"
/transcript_id="NM_001182892.3"
/db_xref="GeneID:855674"
CDS 529942..531411
/gene="MSG5"
/locus_tag="YNL053W"
/EC_number="3.1.3.48"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:17952059|PMID:17963515]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:17963515|PMID:17952059|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008138 protein
tyrosine/serine/threonine phosphatase activity
[PMID:8306972]"
/experiment="EXISTENCE:direct assay:GO:0071852 fungal-type
cell wall organization or biogenesis [PMID:14703512]"
/experiment="EXISTENCE:direct assay:GO:1903138 negative
regulation of cell integrity MAPK cascade
[PMID:14703512|PMID:8306972]"
/experiment="EXISTENCE:genetic interaction:GO:0071852
fungal-type cell wall organization or biogenesis
[PMID:10625705|PMID:11923319]"
/experiment="EXISTENCE:genetic interaction:GO:1903138
negative regulation of cell integrity MAPK cascade
[PMID:10625705|PMID:11923319]"
/experiment="EXISTENCE:mutant phenotype:GO:0060237
regulation of fungal-type cell wall organization
[PMID:14703512]"
/experiment="EXISTENCE:mutant phenotype:GO:0071507
pheromone response MAPK cascade [PMID:8306972]"
/experiment="EXISTENCE:mutant phenotype:GO:0071852
fungal-type cell wall organization or biogenesis
[PMID:14703512|PMID:10625705]"
/experiment="EXISTENCE:mutant phenotype:GO:1903138
negative regulation of cell integrity MAPK cascade
[PMID:10625705|PMID:8306972|PMID:14703512]"
/note="Dual-specificity protein phosphatase; maintains low
levels of both basal and induced cell integrity pathway
signaling by dephosphorylation of the Slt2p MAPK;
reciprocally regulated by Slt2p through phosphorylation;
minor role with Ptp2p in the adaptive response to
pheromone through the dephosphorylation of the Fus3p MAPK
with major contribution by Ptp3p; inhibits the nuclear
accumulation of Fus3p; two isoforms exist as the result of
alternative translation initiation starts"
/codon_start=1
/product="tyrosine/serine/threonine protein phosphatase
MSG5"
/protein_id="NP_014345.3"
/db_xref="GeneID:855674"
/db_xref="SGD:S000004998"
/translation="MQFHSDKQHLDSKTDIDFKPNSPRSLQNRNTKNLSLDIAALHPL
MEFSSPSQDVPGSVKFPSPTPLNLFMKPKPIVLEKCPPKVSPRPTPPSLSMRRSEASI
YTLPTSLKNRTVSPSVYTKSSTVSSISKLSSSSPLSSFSEKPHLNRVHSLSVKTKDLK
LKGIRGRSQTISGLETSTPISSTREGTLDSTDVNRFSNQKNMQTTLIFPEEDSDLNID
MVHAEIYQRTVYLDGPLLVLPPNLYLYSEPKLEDILSFDLVINVAKEIPNLEFLIPPE
MAHKIKYYHIEWTHTSKIVKDLSRLTRIIHTAHSQGKKILVHCQCGVSRSASLIVAYI
MRYYGLSLNDAYNKLKGVAKDISPNMGLIFQLMEWGTMLSKNSPGEEGETVHMPEEDD
IGNNEVSSTTKSYSSASFRSFPMVTNLSSSPNDSSVNSSEVTPRTPATLTGARTALAT
ERGEDDEHCKSLSQPADSLEASVDNESISTAPEQMMFLP"
gene <531725..>532186
/gene="COX5A"
/locus_tag="YNL052W"
/db_xref="GeneID:855675"
mRNA <531725..>532186
/gene="COX5A"
/locus_tag="YNL052W"
/product="cytochrome c oxidase subunit Va"
/transcript_id="NM_001182891.1"
/db_xref="GeneID:855675"
CDS 531725..532186
/gene="COX5A"
/locus_tag="YNL052W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0006123
mitochondrial electron transport, cytochrome c to oxygen
[PMID:1331058]"
/experiment="EXISTENCE:direct assay:GO:0045277 respiratory
chain complex IV [PMID:1331058]"
/experiment="EXISTENCE:mutant phenotype:GO:0004129
cytochrome-c oxidase activity [PMID:1847916]"
/note="Subunit Va of cytochrome c oxidase; cytochrome c
oxidase is the terminal member of the mitochondrial inner
membrane electron transport chain; Cox5Ap is predominantly
expressed during aerobic growth while its isoform Vb
(Cox5Bp) is expressed during anaerobic growth; COX5A has a
paralog, COX5B, that arose from the whole genome
duplication"
/codon_start=1
/product="cytochrome c oxidase subunit Va"
/protein_id="NP_014346.1"
/db_xref="GeneID:855675"
/db_xref="SGD:S000004997"
/translation="MLRNTFTRAGGLSRITSVRFAQTHALSNAAVMDLQSRWENMPST
EQQDIVSKLSERQKLPWAQLTEPEKQAVWYISYGEWGPRRPVLNKGDSSFIAKGVAAG
LLFSVGLFAVVRMAGGQDAKTMNKEWQLKSDEYLKSKNANPWGGYSQVQSK"
gene <532659..>533870
/gene="COG5"
/locus_tag="YNL051W"
/gene_synonym="API4; COD4"
/db_xref="GeneID:855676"
mRNA <532659..>533870
/gene="COG5"
/locus_tag="YNL051W"
/gene_synonym="API4; COD4"
/product="Golgi transport complex subunit COG5"
/transcript_id="NM_001182890.1"
/db_xref="GeneID:855676"
CDS 532659..533870
/gene="COG5"
/locus_tag="YNL051W"
/gene_synonym="API4; COD4"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0006891
intra-Golgi vesicle-mediated transport [PMID:11703943]"
/experiment="EXISTENCE:mutant phenotype:GO:0017119 Golgi
transport complex [PMID:11703943]"
/experiment="EXISTENCE:mutant phenotype:GO:0032258
cytoplasm to vacuole targeting by the Cvt pathway
[PMID:20065092]"
/note="Component of the conserved oligomeric Golgi
complex; a cytosolic tethering complex (Cog1p through
Cog8p) that functions in protein trafficking to mediate
fusion of transport vesicles to Golgi compartments"
/codon_start=1
/product="Golgi transport complex subunit COG5"
/protein_id="NP_014347.1"
/db_xref="GeneID:855676"
/db_xref="SGD:S000004996"
/translation="MTIAPMANDLEDFESLLEPDFDAKQFGNDLLKATNNNDTTILDL
NTPLKKLNYDLHEIDSRIDQLMNSNPLEIIELIYKNEHVNSTIVGELKPSLGYMNMSY
DRLKNQVLDPYERARKVQLALSKVYQTSFLLRGALLYIHLSNKLNALSKTAQLSTSTA
INLASLHYQLEITLEENKNLKSLRKIKQLDQDIVSPNKRELITFLSLQMCKECLNSIK
IKSNKEIISQLAYSLYLLSSQEFESAINKIVLSNVTMSSQILSKILNSIRMFPDAFNE
VVEKGYNIYILETLLQNIKTDNVTNSSRSIAANKSRLGNLLSEYTSMKSKAGSGTPRD
LFWSKVSSAFKKDFDISVNRGGPVGKSLLKNKDFIINTMKQSMKKSSDNSDYQSYLDV
MLNSVSISLNK"
gene complement(<534079..>534982)
/locus_tag="YNL050C"
/db_xref="GeneID:855677"
mRNA complement(join(<534079..534874,534966..>534982))
/locus_tag="YNL050C"
/product="uncharacterized protein"
/transcript_id="NM_001182889.3"
/db_xref="GeneID:855677"
CDS complement(join(534079..534874,534966..534982))
/locus_tag="YNL050C"
/note="hypothetical protein; YNL050c is not an essential
gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014348.3"
/db_xref="GeneID:855677"
/db_xref="SGD:S000004995"
/translation="MSEFKIVSRKDLYNEGEGLGEDYDSNSSSKNNSEHVEVLVPPTE
FEFVEVERTDSSLDLKESNNSAHEQKEEKQEEFEFPLFSFGVVEASTSPAQEEQGSST
QEKDTPQTEVSLMKISLKEPEEEIINQERPKDYYFASYSADQKLQFQQSSIDYDVIIQ
ESTKILEDDLRIRDKWPYCQGRIIDLYKHNARIELEQQKELKIKKRRPGQKQRAAKKL
ALERTKERDTKAREIKKQLKKKFHKRGGKKNKKKVPLNPLAKAGSTPKFRTE"
gene complement(<535281..>537911)
/gene="SFB2"
/locus_tag="YNL049C"
/gene_synonym="ISS1"
/db_xref="GeneID:855678"
mRNA complement(<535281..>537911)
/gene="SFB2"
/locus_tag="YNL049C"
/gene_synonym="ISS1"
/product="COPII subunit SFB2"
/transcript_id="NM_001182888.1"
/db_xref="GeneID:855678"
CDS complement(535281..537911)
/gene="SFB2"
/locus_tag="YNL049C"
/gene_synonym="ISS1"
/experiment="EXISTENCE:direct assay:GO:0090110
COPII-coated vesicle cargo loading [PMID:10712514]"
/experiment="EXISTENCE:genetic interaction:GO:0090110
COPII-coated vesicle cargo loading
[PMID:10712514|PMID:12941277]"
/experiment="EXISTENCE:physical interaction:GO:0030127
COPII vesicle coat [PMID:10753972|PMID:10749860]"
/note="Component of the Sec23p-Sfb2p heterodimer of the
COPII vesicle coat; required for cargo selection during
vesicle formation in ER to Golgi transport; homologous to
Sfb3p; SFB2 has a paralog, SEC24, that arose from the
whole genome duplication"
/codon_start=1
/product="COPII subunit SFB2"
/protein_id="NP_014349.1"
/db_xref="GeneID:855678"
/db_xref="SGD:S000004994"
/translation="MSHHKKRVYPQAQVPYIASMPIVAEQQQSQQQIDQTAYAMGNLQ
LNNRANSFTQLAQNQQFPGSGKVVNQLYPVDLFTELPPPIRDLSLPPLPITISQDNIV
TPSEYSNVPYQYVRSTLKAVPKTNSLLKKTKLPFAIVIRPYLHLQDSDNQVPLNTDGV
IVRCRRCRSYMNPFVVFINQGRKWQCNICRFKNDVPFGFDQNLQGAPINRYERNEIKN
SVVDYLAPVEYSVREPPPSVYVFLLDVSQNAVKNGLLATSARTILENIEFLPNHDGRT
RIAIICVDHSLHYFYVPLDDDYEVSDEDDEESDGEEEDEDEEEEDVDNSETIQMFDIG
DLDEPFLPMPSDELVVPLKYCKNNLETLLKKIPEIFQDTHSSKFALGPALKAASNLIK
STGGKVEVISSTLPNTGIGKLKKRSEQGILNTPKESSQLLSCKDSFYKTFTIECNKLQ
ITVDMFLASEDYMDVATLSHLGRFSGGQTHFYPGFNATSLNDVTKFTRELSRHLSMDI
SMEAVMRVRCSTGLRATSFFGHFFNRSSDLCAFSTMPRDQSYLFGISIEDSLMAEYCY
LQVSTLLTLNTGERRIRVMTLALPTSESAREVFASADQLAITDFMTQNAVTKALNSSM
YSARDFITKSLEDILNAYKKEISMSNINSVTSLNLCANLRMLPLLMNGLSKHIALRPG
VVPSDYRASALNRLETEPLHYLIKSIYPTVYSLHDMPDEVGLPDFEGKTVLPEPINAT
ISLFERYGLYLIDNSAELFLWVGGDAVPELLIDVFNTDTISQIPVGKSELPLLNDSPF
NERLRRIIGRIRENNDTITFQSLYIIRGPSINEPANLNSEKDMASLRLWVLSTLVEDK
VLNCASYREYLQSMKTSINR"
gene <538173..>539819
/gene="ALG11"
/locus_tag="YNL048W"
/db_xref="GeneID:855679"
mRNA <538173..>539819
/gene="ALG11"
/locus_tag="YNL048W"
/product="alpha-1,2-mannosyltransferase ALG11"
/transcript_id="NM_001182887.1"
/db_xref="GeneID:855679"
CDS 538173..539819
/gene="ALG11"
/locus_tag="YNL048W"
/EC_number="2.4.1.131"
/experiment="EXISTENCE:direct assay:GO:0004377
GDP-Man:Man(3)GlcNAc(2)-PP-Dol
alpha-1,2-mannosyltransferase activity [PMID:16878994]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:11278778]"
/experiment="EXISTENCE:direct assay:GO:0006488
dolichol-linked oligosaccharide biosynthetic process
[PMID:16878994]"
/experiment="EXISTENCE:mutant phenotype:GO:0000026
alpha-1,2-mannosyltransferase activity [PMID:11278778]"
/experiment="EXISTENCE:mutant phenotype:GO:0009101
glycoprotein biosynthetic process [PMID:11278778]"
/note="Alpha-1,2-mannosyltransferase; catalyzes sequential
addition of the two terminal alpha 1,2-mannose residues to
the Man5GlcNAc2-PP-dolichol intermediate during
asparagine-linked glycosylation in the ER"
/codon_start=1
/product="alpha-1,2-mannosyltransferase ALG11"
/protein_id="NP_014350.1"
/db_xref="GeneID:855679"
/db_xref="SGD:S000004993"
/translation="MGSAWTNYNFEEVKSHFGFKKYVVSSLVLVYGLIKVLTWIFRQW
VYSSLNPFSKKSSLLNRAVASCGEKNVKVFGFFHPYCNAGGGGEKVLWKAVDITLRKD
AKNVIVIYSGDFVNGENVTPENILNNVKAKFDYDLDSDRIFFISLKLRYLVDSSTWKH
FTLIGQAIGSMILAFESIIQCPPDIWIDTMGYPFSYPIIARFLRRIPIVTYTHYPIMS
KDMLNKLFKMPKKGIKVYGKILYWKVFMLIYQSIGSKIDIVITNSTWTNNHIKQIWQS
NTCKIIYPPCSTEKLVDWKQKFGTAKGERLNQAIVLAQFRPEKRHKLIIESFATFLKN
LPDSVSPIKLIMAGSTRSKQDENYVKSLQDWSENVLKIPKHLISFEKNLPFDKIEILL
NKSTFGVNAMWNEHFGIAVVEYMASGLIPIVHASAGPLLDIVTPWDANGNIGKAPPQW
ELQKKYFAKLEDDGETTGFFFKEPSDPDYNTTKDPLRYPNLSDLFLQITKLDYDCLRV
MGARNQQYSLYKFSDLKFDKDWENFVLNPICKLLEEEERG"
gene complement(<539911..>541881)
/gene="SLM2"
/locus_tag="YNL047C"
/gene_synonym="LIT1"
/db_xref="GeneID:855680"
mRNA complement(<539911..>541881)
/gene="SLM2"
/locus_tag="YNL047C"
/gene_synonym="LIT1"
/product="phosphatidylinositol 4,5-bisphosphate-binding
protein"
/transcript_id="NM_001182886.3"
/db_xref="GeneID:855680"
CDS complement(539911..541881)
/gene="SLM2"
/locus_tag="YNL047C"
/gene_synonym="LIT1"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:15689497|PMID:15372071]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0001558
regulation of cell growth [PMID:15689497]"
/experiment="EXISTENCE:genetic interaction:GO:0016197
endosomal transport [PMID:21451250]"
/experiment="EXISTENCE:genetic interaction:GO:0030036
actin cytoskeleton organization [PMID:15372071]"
/experiment="EXISTENCE:genetic interaction:GO:0051017
actin filament bundle assembly [PMID:15689497]"
/experiment="EXISTENCE:genetic interaction:GO:0070941
eisosome assembly [PMID:21451250]"
/experiment="EXISTENCE:genetic interaction:GO:0072659
protein localization to plasma membrane [PMID:22307609]"
/experiment="EXISTENCE:physical interaction:GO:0001558
regulation of cell growth [PMID:15689497]"
/experiment="EXISTENCE:physical interaction:GO:0030950
establishment or maintenance of actin cytoskeleton
polarity [PMID:15689497]"
/experiment="EXISTENCE:physical interaction:GO:0031929 TOR
signaling [PMID:15689497]"
/note="Phosphoinositide PI4,5P(2) binding protein, forms a
complex with Slm1p; acts downstream of Mss4p in a pathway
regulating actin cytoskeleton organization in response to
stress; TORC2 complex substrate and effector; SLM2 has a
paralog, SLM1, that arose from the whole genome
duplication"
/codon_start=1
/product="phosphatidylinositol 4,5-bisphosphate-binding
protein"
/protein_id="NP_014351.3"
/db_xref="GeneID:855680"
/db_xref="SGD:S000004992"
/translation="MSYQRNSARASLDLRSQYQQLEGRMRSEHFNPAYQQQQQKGQNI
PLSLPSSLAQRNPIPYPIDAVTSDPTIPAQLNVYDHDRQNSIVDAAAGTNTTHSLNSN
NIPSSQNNNINNNNINNVGSFTDPSMLTLPKMSLHSHQKQYDSNQNDPRSPLAILIPT
SAQPTDVLSARFSAWRNVIRAILVYLSETASIQDEIVRQQLRLSHAVQFPFFSIENQY
QPVSNEDKSMQKFFLPLGSGSVQDLPTMLTKYHDNLASLASKSSKELTSEIIPRLEDL
RRDLLVKIKEIKALQSDFKNSCNKELQQTKHLMKLFNESLKECKLGTPKSDPFLIKLQ
LEKQIKRQLVEENYLHEAFDNLQNSGAQLESVIVMEIQNGLTSYARILGKEAQVVFDS
VISKLDSTILNKNTNLEWDSFILRNISNFVPPNLPMRRFKEISYSNQNDPFTFEVKSG
FLEKRSKFLKSYSRGFYVLTPSFLHEFKTPDKHKFSTPLMSIPLVECTVTEHSKKTKS
NSEQGKNKFILRTNSNGLIHRGHNWVFKVDSYDDMIEWFGNIKALSSLPNYDDKCKYV
SKVAKLSKEKAKSNENTTESVTPQVTNEQHTRYDDVSSSNFPLNSIPKLDNLTITNTT
SSIPETNDSQIQNRVPEFYIENVDSPRKSNQL"
gene <542304..>542822
/locus_tag="YNL046W"
/db_xref="GeneID:855681"
mRNA <542304..>542822
/locus_tag="YNL046W"
/product="uncharacterized protein"
/transcript_id="NM_001182885.3"
/db_xref="GeneID:855681"
CDS 542304..542822
/locus_tag="YNL046W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095]"
/note="hypothetical protein; expression depends on Swi5p;
GFP-fusion protein localizes to the endoplasmic reticulum;
deletion confers sensitivity to
4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014352.3"
/db_xref="GeneID:855681"
/db_xref="SGD:S000004991"
/translation="MEHVSKRSIGQFFKRKTSTVDGSKSQKCGTTNQLRKLLHKRRVQ
KQAVPVESQYRIPGDFRDNQSVRVKNSMYNSSPSVTPSTHHINERYVRYDINTRPLVV
VLAISIVFFGCLLVLKDIIIQSSENILSVSKWKIIGASFMGTPYTGLLTGLVGPLLSP
FSAVSSWLSFIF"
gene <542963..>544978
/gene="LAP2"
/locus_tag="YNL045W"
/db_xref="GeneID:855682"
mRNA <542963..>544978
/gene="LAP2"
/locus_tag="YNL045W"
/product="bifunctional aminopeptidase/epoxide hydrolase"
/transcript_id="NM_001182884.1"
/db_xref="GeneID:855682"
CDS 542963..544978
/gene="LAP2"
/locus_tag="YNL045W"
/EC_number="3.3.2.10"
/experiment="EXISTENCE:direct assay:GO:0004177
aminopeptidase activity [PMID:10574934]"
/experiment="EXISTENCE:direct assay:GO:0004301 epoxide
hydrolase activity [PMID:10574934]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006629 lipid
metabolic process [PMID:10574934]"
/experiment="EXISTENCE:direct assay:GO:0030163 protein
catabolic process [PMID:10574934]"
/note="Leucyl aminopeptidase yscIV with epoxide hydrolase
activity; metalloenzyme containing one zinc atom; green
fluorescent protein (GFP)-fusion protein localizes to the
cytoplasm and nucleus; also known as leukotriene A4
hydrolase"
/codon_start=1
/product="bifunctional aminopeptidase/epoxide hydrolase"
/protein_id="NP_014353.1"
/db_xref="GeneID:855682"
/db_xref="SGD:S000004990"
/translation="MFLLPFVIRHSSSIYLPTLRFRGLLTVISRNIHISTPHKMLPLS
IEQRRPSRSPEYDQSTLSNYKDFAVLHTDLNLSVSFEKSAISGSVTFQLKKLHEGKNK
SDELHLDTSYLDVQEVHIDGSKADFQIEQRKEPLGSRLVINNASCNDNFTLNIQFRTT
DKCTALQWLNSKQTKGGKPYVFSQLEAIHARSLFPCFDTPSVKSTFTASIESPLPVVF
SGIRIEDTSKDTNIYRFEQKVPIPAYLIGIASGDLSSAPIGPRSTVYTEPFRLKDCQW
EFENDVEKFIQTAEKIIFEYEWGTYDILVNVDSYPYGGMESPNMTFATPTLIAHDRSN
IDVIAHELAHSWSGNLVTNCSWNHFWLNEGWTVYLERRIIGAIHGEPTRHFSALIGWS
DLQNSIDSMKDPERFSTLVQNLNDNTDPDDAFSTVPYEKGFNLLFHLETILGGKAEFD
PFIRHYFKKFAKKSLDTFQFLDTLYEFYPEKKEILDSVDWETWLYKPGMPPRPHFITA
LADNVYQLADKWVEMAQHLKTTEDFRSEFNAIDIKDFNSNQLVLFLETLTQNGHSNKK
PKDFDWAKFPVASRALLDIYQDNIVKSQNAEVVFKMFKFQIFAKLQEEYKHLADWLGT
VGRMKFVRPGYRLLNSVDRRLALATFDKFKDTYHPICKALVKQDLGL"
gene <545268..>545877
/gene="YIP3"
/locus_tag="YNL044W"
/db_xref="GeneID:855683"
mRNA join(<545268..545291,545371..>545877)
/gene="YIP3"
/locus_tag="YNL044W"
/product="Yip3p"
/transcript_id="NM_001182883.1"
/db_xref="GeneID:855683"
CDS join(545268..545291,545371..545877)
/gene="YIP3"
/locus_tag="YNL044W"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:35563734]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:16002643]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:16002643]"
/experiment="EXISTENCE:direct assay:GO:0006888 endoplasmic
reticulum to Golgi vesicle-mediated transport
[PMID:11157978]"
/experiment="EXISTENCE:direct assay:GO:0030134
COPII-coated ER to Golgi transport vesicle
[PMID:11157978]"
/note="Protein localized to COPII vesicles; proposed to be
involved in ER-to-Golgi transport; interacts with members
of the Rab GTPase family and Yip1p; also interacts with
Rtn1p; integral membrane protein that localizes to the ER,
the Golgi apparatus, transport vesicles and the
peroxisome; two isoforms exist, a full-length Yip3L (Yip3
Long) and a shorter peroxisomal matrix isoform Yip3S (Yip3
Short)"
/codon_start=1
/product="Yip3p"
/protein_id="NP_014354.2"
/db_xref="GeneID:855683"
/db_xref="SGD:S000004989"
/translation="MNQLGALAQVSRFTQNFSMENIKSEFQSLQSKLATLRTPQEFFN
FKKISKPQNFGEVQSRVAYNLKYFSSNYGLIIGCLSIYTLLTNLLLLFVIVLVVAGIV
GINKLKGEELVTPFGSFKTNQLYTGLVCVAVPIGFLASPISTLLWLIGASAVSVFGHA
SLMEKPIETVFDEETV"
rep_origin 546072..546170
/note="ARS1421; Autonomously Replicating Sequence"
/db_xref="SGD:S000118389"
repeat_region 546423..546714
/note="Ty3 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007138"
repeat_region 546738..547077
/note="Ty3 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007139"
gene 547094..547196
/locus_tag="YNCN0009W"
/db_xref="GeneID:855685"
tRNA join(547094..547129,547161..547196)
/locus_tag="YNCN0009W"
/product="tRNA-Pro"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE
analysis; target of K. lactis zymocin"
/db_xref="GeneID:855685"
/db_xref="SGD:S000006685"
gene <547113..>547370
/locus_tag="YNL042W-B"
/db_xref="GeneID:1466515"
mRNA <547113..>547370
/locus_tag="YNL042W-B"
/product="uncharacterized protein"
/transcript_id="NM_001184662.3"
/db_xref="GeneID:1466515"
CDS 547113..547370
/locus_tag="YNL042W-B"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878157.3"
/db_xref="GeneID:1466515"
/db_xref="SGD:S000028850"
/translation="MILALGDFLPKQEDKACERPWVQFPARPVIFFHHQGGIFLFSIN
QPNLSCFSKLKEVNSLYVRVATYICQKNESRFRTNRLKGDQ"
gene <548100..>549290
/gene="BOP3"
/locus_tag="YNL042W"
/db_xref="GeneID:855686"
mRNA <548100..>549290
/gene="BOP3"
/locus_tag="YNL042W"
/product="Bop3p"
/transcript_id="NM_001182881.1"
/db_xref="GeneID:855686"
CDS 548100..549290
/gene="BOP3"
/locus_tag="YNL042W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0071406
cellular response to methylmercury [PMID:15796894]"
/note="hypothetical protein; potential Cdc28p substrate;
overproduction confers resistance to methylmercury"
/codon_start=1
/product="Bop3p"
/protein_id="NP_014356.1"
/db_xref="GeneID:855686"
/db_xref="SGD:S000004987"
/translation="MSTFNSYSQPKESNDNSHNNVNKSKSLLDIIFGTNVSEWAFSEN
ALMKAMDLKIEQEKTKQQYYKLENLNRSIELFKLASSSGLPINQIHKLFNTDHGVPAS
SPMKAGGNQPHNNTEGTQSSENLPRLNGSMKSLKPLNMNTVSPTPMSRQPSPYKFPAS
SSTGGISHSTVTNVQRRANSPARIGASAVAALNDNISIKEEDVARRIPSGTKSQESPL
NKKPTSLHSRNLSLPIGKFTNPNIPSTMTSILSFNRDQQQPLSQPLPPPPQQQQDLHT
HNLHTIPRKPGMVQKKHRRARSTSSFGVIDLSIIDEAKEKQVQRSPSPIHSNVSVALT
SHDKPIESNMKEQPNMLQSVREGRQVHDDLDDRTCSESSSRNESPVRTITKDNSVGKI
LNST"
gene complement(<549468..>551987)
/gene="COG6"
/locus_tag="YNL041C"
/gene_synonym="COD2; SEC37"
/db_xref="GeneID:855687"
mRNA complement(<549468..>551987)
/gene="COG6"
/locus_tag="YNL041C"
/gene_synonym="COD2; SEC37"
/product="Golgi transport complex subunit COG6"
/transcript_id="NM_001182880.1"
/db_xref="GeneID:855687"
CDS complement(549468..551987)
/gene="COG6"
/locus_tag="YNL041C"
/gene_synonym="COD2; SEC37"
/experiment="EXISTENCE:mutant phenotype:GO:0006891
intra-Golgi vesicle-mediated transport [PMID:11703943]"
/experiment="EXISTENCE:mutant phenotype:GO:0017119 Golgi
transport complex [PMID:11703943]"
/experiment="EXISTENCE:mutant phenotype:GO:0032258
cytoplasm to vacuole targeting by the Cvt pathway
[PMID:20065092]"
/note="Component of the conserved oligomeric Golgi
complex; a cytosolic tethering complex (Cog1p through
Cog8p) that functions in protein trafficking to mediate
fusion of transport vesicles to Golgi compartments"
/codon_start=1
/product="Golgi transport complex subunit COG6"
/protein_id="NP_014357.1"
/db_xref="GeneID:855687"
/db_xref="SGD:S000004986"
/translation="MDFVVDYQTYAMADTATPELPEPEPRLNLTSDAQSQPTGKLDLQ
FKLPDLQRYSNNNATLPVDNDGAGSKDLHKKMTHYAMSSIDKIQLSNPSKQLGQNSQD
EKLSQQESQNFTNYEPKNLDLSKLVSPSSGSNKNTTNLVLSNKLSKILNNYTLINYQA
TVQLRKSLKVLEENKERLSLDEQKLMNPEYVGTLARRALRTDLESQLLKEHITVLEEF
KPIIRRIKRLSSSVEKIQRTSEKLLSNETNEVPTNNVVLQEIDQYRLKAEQLKLKKKI
LLSIRDRFTLNQVEDDVITNGTIDNIFFEVVKKVINIKDESSFLLTLPNLNAGNALIM
GVNEILEKTNKKIFNYLIDFLYSFESSSNLLNDHGTTEQESLNIFRKSLVFLSSDLEL
FNELLKRVTTLRSKSILDEFLSQFDMNSTTSKPIILSAHDPIRYIGDVLASVHSIIAN
EADFVKSLFDFQDEDLKDTPISILQQNKTFLKGIDNKLLNDIIQSLSNSCRIRIEQIV
RFEENPIINFEIVRLLKLYRVMFERKGIQDDSSIINNLKSLEDISKNRIIGYYEDYMK
QTVMAETKNSSDDLLPPEWLSEYMNKLVELFEIYEKTHAAEDEESEDNKLLSSKNLQT
IVEQPIKDVLLKQLQTSFPLAKKNEKEKASLLTIEINCFDLIKSRLQPFEGLFAQDDD
SRKITIWVCDKLKEYTKQMLTLQIKFLFENTGLDLYSNLVNMIFPVDSVKDELDYDMY
LALRDNSLMELDMVRKNVHDKLNYYLPQALTDVQGNLLFKLTSPMIADEICDECFKKL
SLFYNIFRKLLIHLYPNKKDQVFEILNFSTDEFDMLIGIDH"
gene complement(<552478..>552558)
/locus_tag="YNL040C-A"
/db_xref="GeneID:91000625"
mRNA complement(<552478..>552558)
/locus_tag="YNL040C-A"
/product="uncharacterized protein"
/transcript_id="NM_001431143.1"
/db_xref="GeneID:91000625"
CDS complement(552478..552558)
/locus_tag="YNL040C-A"
/note="hypothetical protein; deletion confers sensitivity
to osmotic stress"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001418072.1"
/db_xref="GeneID:91000625"
/db_xref="SGD:S000350098"
/translation="MPEQKANCSPNGNITVDSMIMSLGSS"
gene <553380..>554750
/locus_tag="YNL040W"
/db_xref="GeneID:855688"
mRNA <553380..>554750
/locus_tag="YNL040W"
/product="putative alanine--tRNA ligase"
/transcript_id="NM_001182879.3"
/db_xref="GeneID:855688"
CDS 553380..554750
/locus_tag="YNL040W"
/experiment="EXISTENCE:direct assay:GO:0002196
Ser-tRNA(Ala) deacylase activity [PMID:38869066]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0043905
L-seryl-tRNA(Thr) hydrolase activity [PMID:38869066]"
/experiment="EXISTENCE:genetic interaction:GO:0106074
aminoacyl-tRNA metabolism involved in translational
fidelity [PMID:38869066]"
/experiment="EXISTENCE:mutant phenotype:GO:0106074
aminoacyl-tRNA metabolism involved in translational
fidelity [PMID:38869066]"
/note="Ser-tRNA(Ala) hydrolase; hydrolyzes Ser-mischarged
cytoplasmic and mitochondrial tRNAs; regulates
translational fidelity based on Ala- and Thr-to-Ser
misincorporation in the null mutant; trans-editing factor
for alanyl- and threonyl-tRNA synthetases and seryl-tRNA
synthetase (SerRS) via hydrolysis; null mutant displays
decreased translation rate and increased readthrough of
premature stop codons; (GFP)-fusion protein localizes to
the cytoplasm; homologous to human trans-editing factor
AARSD"
/codon_start=1
/product="putative alanine--tRNA ligase"
/protein_id="NP_014358.3"
/db_xref="GeneID:855688"
/db_xref="SGD:S000004985"
/translation="MPTPMTPVKVGALACQRNSFLFDGFKTLVVSCEPTKNKKGEIEG
YEIELQDTILFPEGGGQPSDSGFLKIVEGNRNSSKIEKILVSHVSRFGLHAKHHVNDY
IEPGTTVEVAVDEQKRMDYMQQHTGQHLLSAILERNYKVDTVSWSMGGIITKKKPVLE
PSDYFNYIELNRKLTLDEITNVSDEINQLIINFPQEIIVEERIGEETVDEVSTSKIPD
DYDLSKGVLRTIHIGDIDSNPCCGTHLKCTSQIGSILILSNQSAVRGSNSRLYFMCGK
RVSLYAKSVNKILLDSKNLLSCSETQISEKITRQTKQIQQLNKREQYWIKRLARTASE
ELMNTLKASGKKRAYFMEEEYGTLELLLQIHKEVSNFLKDDTEGYEIILCGYERQTNT
GSLLILSESGEKIANLAANLGSILQNLKGGGGKKGGKWQGKITSISNAEFAALSDYLS
HDFASC"
gene <555048..>556832
/gene="BDP1"
/locus_tag="YNL039W"
/gene_synonym="TFC5; TFC7"
/db_xref="GeneID:855689"
mRNA <555048..>556832
/gene="BDP1"
/locus_tag="YNL039W"
/gene_synonym="TFC5; TFC7"
/product="transcription factor TFIIIB subunit BDP1"
/transcript_id="NM_001182878.3"
/db_xref="GeneID:855689"
CDS 555048..556832
/gene="BDP1"
/locus_tag="YNL039W"
/gene_synonym="TFC5; TFC7"
/experiment="EXISTENCE:direct assay:GO:0000126
transcription factor TFIIIB complex
[PMID:7568218|PMID:1458536]"
/experiment="EXISTENCE:direct assay:GO:0001156
TFIIIC-class transcription factor complex binding
[PMID:12930823]"
/experiment="EXISTENCE:direct assay:GO:0070898 RNA
polymerase III preinitiation complex assembly
[PMID:11387215]"
/experiment="EXISTENCE:mutant phenotype:GO:0001112
DNA-templated transcription open complex formation
[PMID:11387215]"
/experiment="EXISTENCE:mutant phenotype:GO:0070898 RNA
polymerase III preinitiation complex assembly
[PMID:11387215]"
/experiment="EXISTENCE:physical interaction:GO:0001156
TFIIIC-class transcription factor complex binding
[PMID:12930823]"
/note="Essential subunit of RNA polymerase III
transcription factor (TFIIIB); TFIIIB is involved in
transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA,
and other small RNAs"
/codon_start=1
/product="transcription factor TFIIIB subunit BDP1"
/protein_id="NP_014359.3"
/db_xref="GeneID:855689"
/db_xref="SGD:S000004984"
/translation="MSSIVNKSGTRFAPKVRQRRAATGGTPTPKPRTPQLFIPESKEI
EEDNSDNDKGVDENETAIVEKPSLVGERSLEGFTLTGTNGHDNEIGDEGPIDASTQNP
KADVIEDNVTLKPAPLQTHRDQKVPRSSRLASLSKDNESRPSFKPSFLDSSSNSNGTA
RRLSTISNKLPKKIRLGSITENDMNLKTFKRHRVLGKPSSAKKPAGAHRISIVSKISP
PTAMTDSLDRNEFSSETSTSREADENENYVISKVKDIPKKVRDGESAKYFIDEENFTM
AELCKPNFPIGQISENFEKSKMAKKAKLEKRRHLRELRMRARQEFKPLHSLTKEEQEE
EEEKRKEERDKLLNADIPESDRKAHTAIQLKLNPDGTMAIDEETMVVDRHKNASIENE
YKEKVDENPFANLYNYGSYGRGSYTDPWTVEEMIKFYKALSMWGTDFNLISQLYPYRS
RKQVKAKFVNEEKKRPILIELALRSKLPPNFDEYCCEIKKNIGTVADFNEKLIELQNE
HKHHMKEIEEAKNTAKEEDQTAQRLNDANLNKKGSGGIMTNDLKVYRKTEVVLGTIDD
LKRKKLKERNNDDNEDNEGSEEEPEIDQ"
gene <557020..>557783
/gene="GPI15"
/locus_tag="YNL038W"
/db_xref="GeneID:855690"
mRNA join(<557020..557610,557685..>557783)
/gene="GPI15"
/locus_tag="YNL038W"
/product="phosphatidylinositol
N-acetylglucosaminyltransferase GPI15"
/transcript_id="NM_001182877.1"
/db_xref="GeneID:855690"
CDS join(557020..557610,557685..557783)
/gene="GPI15"
/locus_tag="YNL038W"
/EC_number="2.4.1.198"
/experiment="EXISTENCE:mutant phenotype:GO:0006506 GPI
anchor biosynthetic process [PMID:11746600]"
/experiment="EXISTENCE:mutant phenotype:GO:0017176
phosphatidylinositol N-acetylglucosaminyltransferase
activity [PMID:11746600]"
/note="Protein involved in the synthesis of GlcNAc-PI;
GlcNAc-PI is the first intermediate in the synthesis of
glycosylphosphatidylinositol (GPI) anchors; homologous to
the human PIG-H protein; GlcNAc-PI stands for
N-acetylglucosaminyl phosphatidylinositol"
/codon_start=1
/product="phosphatidylinositol
N-acetylglucosaminyltransferase GPI15"
/protein_id="NP_014360.2"
/db_xref="GeneID:855690"
/db_xref="SGD:S000004983"
/translation="MISKEYEFGKTSILNRKKYTLVIDEDKNGNFIRFTVLPVSNRKF
KKVKQNGRVEINMGIQYHQIVLILLLNILFYVICLRSRFLEHINRTFEVTIARSFQIL
IIMGLFALGTIILVRGPSVETVTIFKESGLQLSRVKGMVIFPQQWNRKFFEQVEFISN
ERIIDVVINEGFCRGFRVIFYLAAIVRKSSTLKLLFPSNLPSIDDQRLIYNISRKYLS
KQEKPLSRPKD"
gene complement(<557920..>559002)
/gene="IDH1"
/locus_tag="YNL037C"
/db_xref="GeneID:855691"
mRNA complement(<557920..>559002)
/gene="IDH1"
/locus_tag="YNL037C"
/product="isocitrate dehydrogenase (NAD(+)) IDH1"
/transcript_id="NM_001182876.1"
/db_xref="GeneID:855691"
CDS complement(557920..559002)
/gene="IDH1"
/locus_tag="YNL037C"
/EC_number="1.1.1.41"
/experiment="EXISTENCE:direct assay:GO:0004449 isocitrate
dehydrogenase (NAD+) activity [PMID:16884682]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:8626605]"
/experiment="EXISTENCE:direct assay:GO:0006102 isocitrate
metabolic process [PMID:16884682]"
/experiment="EXISTENCE:direct assay:GO:0042645
mitochondrial nucleoid [PMID:15692048]"
/experiment="EXISTENCE:direct assay:GO:0045242 isocitrate
dehydrogenase complex (NAD+) [PMID:16884682]"
/note="Subunit of mitochondrial NAD(+)-dependent
isocitrate dehydrogenase; complex catalyzes the oxidation
of isocitrate to alpha-ketoglutarate in the TCA cycle"
/codon_start=1
/product="isocitrate dehydrogenase (NAD(+)) IDH1"
/protein_id="NP_014361.1"
/db_xref="GeneID:855691"
/db_xref="SGD:S000004982"
/translation="MLNRTIAKRTLATAAQAERTLPKKYGGRFTVTLIPGDGVGKEIT
DSVRTIFEAENIPIDWETINIKQTDHKEGVYEAVESLKRNKIGLKGLWHTPADQTGHG
SLNVALRKQLDIYANVALFKSLKGVKTRIPDIDLIVIRENTEGEFSGLEHESVPGVVE
SLKVMTRPKTERIARFAFDFAKKYNRKSVTAVHKANIMKLGDGLFRNIITEIGQKEYP
DIDVSSIIVDNASMQAVAKPHQFDVLVTPSMYGTILGNIGAALIGGPGLVAGANFGRD
YAVFEPGSRHVGLDIKGQNVANPTAMILSSTLMLNHLGLNEYATRISKAVHETIAEGK
HTTRDIGGSSSTTDFTNEIINKLSTM"
gene <559814..>560479
/gene="NCE103"
/locus_tag="YNL036W"
/gene_synonym="NCE3"
/db_xref="GeneID:855692"
mRNA <559814..>560479
/gene="NCE103"
/locus_tag="YNL036W"
/gene_synonym="NCE3"
/product="carbonate dehydratase NCE103"
/transcript_id="NM_001182875.3"
/db_xref="GeneID:855692"
CDS 559814..560479
/gene="NCE103"
/locus_tag="YNL036W"
/gene_synonym="NCE3"
/EC_number="4.2.1.1"
/experiment="EXISTENCE:direct assay:GO:0004089 carbonate
dehydratase activity [PMID:15096093|PMID:15813743]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005758
mitochondrial intermembrane space [PMID:22984289]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress [PMID:15096093]"
/experiment="EXISTENCE:mutant phenotype:GO:0071244
cellular response to carbon dioxide [PMID:22253597]"
/note="Carbonic anhydrase; metalloenzyme that catalyzes
CO2 hydration to bicarbonate, which is an important
metabolic substrate, and protons; not expressed under
conditions of high CO2, such as inside a growing colony,
but transcription is induced in response to low CO2
levels, such as on the colony surface in ambient air;
poorly transcribed under aerobic conditions and at an
undetectable level under anaerobic conditions; abundance
increases in response to DNA replication stress"
/codon_start=1
/product="carbonate dehydratase NCE103"
/protein_id="NP_014362.3"
/db_xref="GeneID:855692"
/db_xref="SGD:S000004981"
/translation="MSATESSSIFTLSHNSNLQDILAANAKWASQMNNIQPTLFPDHN
AKGQSPHTLFIGCSDSRYNENCLGVLPGEVFTWKNVANICHSEDLTLKATLEFAIICL
KVNKVIICGHTDCGGIKTCLTNQREALPKVNCSHLYKYLDDIDTMYHEESQNLIHLKT
QREKSHYLSHCNVKRQFNRIIENPTVQTAVQNGELQVYGLLYNVEDGLLQTVSTYTKV
TPK"
gene complement(560693..560765)
/locus_tag="YNCN0010C"
/db_xref="GeneID:855693"
tRNA complement(560693..560765)
/locus_tag="YNCN0010C"
/product="tRNA-Thr"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:855693"
/db_xref="SGD:S000006745"
rep_origin 561111..561388
/note="ARS1422; Autonomously Replicating Sequence"
/db_xref="SGD:S000118390"
repeat_region 561607..561977
/note="Ty4 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007141"
repeat_region complement(562033..562364)
/note="Ty2 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007131"
mobile_element complement(562364..567660)
/note="YNLCTy2-1; Ty2 element, LTR retrotransposon of the
Copia (Pseudoviridae) group; contains genes TYA Gag and
TYB Pol, encoding proteins involved in structure and
function of virus-like particles, flanked by two direct
repeats; mutated in S288C"
/mobile_element_type="retrotransposon:YNLCTy2-1"
/db_xref="SGD:S000007135"
repeat_region complement(567660..567992)
/note="Ty2 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007132"
gene 568115..568217
/locus_tag="YNCN0011W"
/db_xref="GeneID:855694"
tRNA join(568115..568150,568182..568217)
/locus_tag="YNCN0011W"
/product="tRNA-Pro"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE
analysis; target of K. lactis zymocin"
/db_xref="GeneID:855694"
/db_xref="SGD:S000006686"
rep_origin 568219..568524
/note="ARS1423; Putative replication origin; identified in
multiple array studies, not yet confirmed by plasmid-based
assay"
/db_xref="SGD:S000130143"
gene complement(<568522..>569691)
/locus_tag="YNL035C"
/db_xref="GeneID:855695"
mRNA complement(<568522..>569691)
/locus_tag="YNL035C"
/product="uncharacterized protein"
/transcript_id="NM_001182874.1"
/db_xref="GeneID:855695"
CDS complement(568522..569691)
/locus_tag="YNL035C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922|PMID:14562095|PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/note="Nuclear hypothetical protein; relocalizes to the
cytosol in response to hypoxia; contains WD-40 domains;
not an essential gene; protein abundance increases in
response to DNA replication stress"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014363.1"
/db_xref="GeneID:855695"
/db_xref="SGD:S000004980"
/translation="MASYSLVESNSFGSENWCLKLQPSYKHGLLTGLSNGEIRLLDWS
TGKSVQKIKASETAINDMKVIGSDFSAGHLVSSASIDAVKVFDIRTNDRIAQIQNEAN
SPFISLDSRHGLLACGTELQGIDAAVYIYDIRKWDTPLRSLIDSHHDDVTCIKFHPSD
VNILLSGSTDGYTNIYDLKQDEEEDALHQVINYASIHSCGWLSPKRIFTLSHMETFAI
HELNDKSDELKEPQPLDFGDVREIWNCDYVVDIYPGLIATGKTQENCGELCLLPFKDE
KVDTENGIVIPHAHGDEVVRDIFIPAQHSNMLYSCGEDGCVKIWENKQGPLDIPENFW
DYSKKMNVLGDDDREGSINLDEPLIIQKESVSTRPRKEKHKKAKKHSMKSRFKPY"
gene complement(569867..569940)
/locus_tag="YNCN0012C"
/db_xref="GeneID:855696"
tRNA complement(569867..569940)
/locus_tag="YNCN0012C"
/product="tRNA-Ile"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Isoleucine tRNA (tRNA-Ile), predicted by
tRNAscan-SE analysis"
/db_xref="GeneID:855696"
/db_xref="SGD:S000006609"
gene <570477..>572315
/locus_tag="YNL034W"
/db_xref="GeneID:855697"
mRNA <570477..>572315
/locus_tag="YNL034W"
/product="uncharacterized protein"
/transcript_id="NM_001182873.1"
/db_xref="GeneID:855697"
CDS 570477..572315
/locus_tag="YNL034W"
/experiment="EXISTENCE:mutant phenotype:GO:0043935 sexual
sporulation resulting in formation of a cellular spore
[PMID:36441813]"
/note="Protein required for sporulation; induced during
meiosis and sporulation; gene is a member of Centroid, a
centromere-linked gene family; not an essential gene;
YNL034W has a paralog, YNL018C, that arose from a
segmental duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014364.1"
/db_xref="GeneID:855697"
/db_xref="SGD:S000004979"
/translation="MSTSFQEFKAFCNKVGLDFQWLNLQSSKSVPESGSSEGFSAVSD
TVQENIRPATEPLNVNQSKDPVSNFFYDVKNAPLWNVYKRNHSGHSSTEACSGVSSGQ
ASKNIPEAMVKETVLSNHDNVTIINELLPTSSAMHQEESTAMTTSYLLSHSVNDSCNV
MLSSSSHNRAMLPPSLVQRNNATTSPTTDSASENNESVPSLTSSVSTSSSVYSSWNPP
HSPHISSFPDGNFASLNAEVTCFDFRRTKDSRTKETNESIIPTEIYCPINSTDHHKHY
PSQKSKQDACAPAPRNQNISCSVGSAAEFSQSNHTLTTVVPSYMQQYLDRPQNWFESK
MGKYCPLFLRSTKNIDYDSLEFKFERKMIAVQYLLLDEQSEPRRYYNPSNKSIPFWKR
PFNFDTMPSYDQLMEEAECRFYSYQYKYEGFQRIEPYSIFCPWKNTQREIDLVLDHIH
FSLDVGEKKSLNRKGNITLDTLDSKVDPNIQIKPYQIFPSNNLVYEGLPHPAEQSLIL
SPGTSLIERAFQALIDICKESIPPSNDCTTRNHNSAPQLTVPEPSKPCRLLLVRESRT
ATELETNKKLWLHSQRRNIEVTVPMHPSEHGTKSRLRKWLSTFVHQ"
gene <572999..>573853
/locus_tag="YNL033W"
/db_xref="GeneID:855698"
mRNA <572999..>573853
/locus_tag="YNL033W"
/product="uncharacterized protein"
/transcript_id="NM_001182872.3"
/db_xref="GeneID:855698"
CDS 572999..573853
/locus_tag="YNL033W"
/note="hypothetical protein; YNL033W has a paralog,
YNL019C, that arose from a segmental duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014365.3"
/db_xref="GeneID:855698"
/db_xref="SGD:S000004978"
/translation="MLYSRESRTTVLFLALVTSLTVLCHSVDVTTVFTTSTITEITTV
TAAPQPQNKAETALNTATNIIQTMQFLFNCAPFKWKGPLKITSCALNFIVLLLTAWGY
LLKYLQENKLNSDADMEKMVGLGFGEMVGRIFGKGVGKAFTKMDITQKLVYPFEGSNR
QKCLLMTVGENSIVPFHDLFTEICFDQYTLDSLSHHNHGSISILDAGSVSALGFADIS
SKMPSVSELYTLFGDYTIEVLGGITKLASTLNREDWQGERNGFAVLSRDRPNQTLLSV
HMYSSSLL"
gene <574506..>575351
/gene="SIW14"
/locus_tag="YNL032W"
/gene_synonym="OCA3"
/db_xref="GeneID:855699"
mRNA <574506..>575351
/gene="SIW14"
/locus_tag="YNL032W"
/gene_synonym="OCA3"
/product="putative tyrosine protein phosphatase SIW14"
/transcript_id="NM_001182871.3"
/db_xref="GeneID:855699"
CDS 574506..575351
/gene="SIW14"
/locus_tag="YNL032W"
/gene_synonym="OCA3"
/EC_number="3.6.1.52"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0052845
inositol-5-diphosphate-1,2,3,4,6-pentakisphosphate
diphosphatase activity [PMID:26828065|PMID:29540476]"
/experiment="EXISTENCE:direct assay:GO:0052847
inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate
5-diphosphatase activity [PMID:29540476]"
/experiment="EXISTENCE:direct assay:GO:0106211
inositol-5-diphosphate-1,3,4,6-tetrakisphosphate
diphosphatase activity [PMID:29540476]"
/experiment="EXISTENCE:mutant phenotype:GO:0007015 actin
filament organization [PMID:15020461]"
/experiment="EXISTENCE:mutant phenotype:GO:0052845
inositol-5-diphosphate-1,2,3,4,6-pentakisphosphate
diphosphatase activity [PMID:29540476|PMID:26828065]"
/experiment="EXISTENCE:mutant phenotype:GO:0052847
inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate
5-diphosphatase activity [PMID:29540476]"
/experiment="EXISTENCE:mutant phenotype:GO:0071543
diphosphoinositol polyphosphate metabolic process
[PMID:26828065]"
/experiment="EXISTENCE:mutant phenotype:GO:0106211
inositol-5-diphosphate-1,3,4,6-tetrakisphosphate
diphosphatase activity [PMID:29540476]"
/note="Inositol phosphatase involved in inositol
pyrophosphate metabolism; hydrolyzes the beta-phosphate
from the 5-diphosphate group of PP-InsPs including:
5PP-IP5 (IP7), 1,5PP-IP4 (IP8) and 5-PP-InsP4; functions
as an anti-prion (anti-[PSI]) component; member of the
atypical dual-specificity subgroup of the protein
tyrosine-phosphatase (PTP) superfamily; localizes to the
cytoplasm"
/codon_start=1
/product="putative tyrosine protein phosphatase SIW14"
/protein_id="NP_014366.3"
/db_xref="GeneID:855699"
/db_xref="SGD:S000004977"
/translation="MGLYQAKNDEGSDPKSSSKIDDLIENEAEIIRLIKEDGKLLIDN
GDGRDIHNIIQEDKLLSVEFNEVLKRFHGEEKSDIPRKEFDEDEDDGYDSNEHHQKTI
EVMNTLNHVINKEVIPPENFSHVVGEIYRSSFPRQENFSFLHERLKLKSILVLIPEEY
PQENLNFLKLTGIKLYQVGMSGNKEPFVNIPSHLLTKALEIVLNPANQPILIHCNRGK
HRTGCLIGCIRKLQNWSLTMIFDEYRRFAFPKARALDQQFIEMYDDDEIKRIASKNNW
LPLQW"
gene complement(<575640..>576050)
/gene="HHT2"
/locus_tag="YNL031C"
/db_xref="GeneID:855700"
mRNA complement(<575640..>576050)
/gene="HHT2"
/locus_tag="YNL031C"
/product="histone H3"
/transcript_id="NM_001182870.1"
/db_xref="GeneID:855700"
CDS complement(575640..576050)
/gene="HHT2"
/locus_tag="YNL031C"
/experiment="EXISTENCE:direct assay:GO:0008823 cupric
reductase (NADH) activity [PMID:32631887]"
/experiment="EXISTENCE:mutant phenotype:GO:0006878
intracellular copper ion homeostasis [PMID:32631887]"
/experiment="EXISTENCE:mutant phenotype:GO:0008823 cupric
reductase (NADH) activity [PMID:32631887]"
/experiment="EXISTENCE:mutant phenotype:GO:0009060 aerobic
respiration [PMID:32631887]"
/note="Histone H3; core histone protein required for
chromatin assembly, part of heterochromatin-mediated
telomeric and HM silencing; one of two identical histone
H3 proteins (see HHT1); regulated by acetylation,
methylation, and phosphorylation; H3K14 acetylation plays
an important role in the unfolding of strongly positioned
nucleosomes during repair of UV damage"
/codon_start=1
/product="histone H3"
/protein_id="NP_014367.1"
/db_xref="GeneID:855700"
/db_xref="SGD:S000004976"
/translation="MARTKQTARKSTGGKAPRKQLASKAARKSAPSTGGVKKPHRYKP
GTVALREIRRFQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAIGALQESVEAYLV
SLFEDTNLAAIHAKRVTIQKKDIKLARRLRGERS"
gene <576727..>577038
/gene="HHF2"
/locus_tag="YNL030W"
/db_xref="GeneID:855701"
mRNA <576727..>577038
/gene="HHF2"
/locus_tag="YNL030W"
/product="histone H4"
/transcript_id="NM_001182869.1"
/db_xref="GeneID:855701"
CDS 576727..577038
/gene="HHF2"
/locus_tag="YNL030W"
/note="Histone H4; core histone protein required for
chromatin assembly and chromosome function; one of two
identical histone proteins (see also HHF1); contributes to
telomeric silencing; N-terminal domain involved in
maintaining genomic integrity"
/codon_start=1
/product="histone H4"
/protein_id="NP_014368.1"
/db_xref="GeneID:855701"
/db_xref="SGD:S000004975"
/translation="MSGRGKGGKGLGKGGAKRHRKILRDNIQGITKPAIRRLARRGGV
KRISGLIYEEVRAVLKSFLESVIRDSVTYTEHAKRKTVTSLDVVYALKRQGRTLYGFG
G"
gene complement(<577205..>578773)
/gene="KTR5"
/locus_tag="YNL029C"
/db_xref="GeneID:855703"
mRNA complement(<577205..>578773)
/gene="KTR5"
/locus_tag="YNL029C"
/product="putative mannosyltransferase"
/transcript_id="NM_001182868.3"
/db_xref="GeneID:855703"
CDS complement(577205..578773)
/gene="KTR5"
/locus_tag="YNL029C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0035269 protein
O-linked glycosylation via mannose [PMID:9090056]"
/note="Putative mannosyltransferase involved in protein
glycosylation; member of the KRE2/MNT1 mannosyltransferase
family; KTR5 has a paralog, KTR7, that arose from the
whole genome duplication"
/codon_start=1
/product="putative mannosyltransferase"
/protein_id="NP_014369.3"
/db_xref="GeneID:855703"
/db_xref="SGD:S000004974"
/translation="MLLIRRTINAFLGCIHCNLTATCILIAFVITMYVVLVSEPASVD
GTMGNFLPFSKMDLATKRDRPFYSNCVNTQDYLLNPSYIKQNASFVMLTRNGELEDVI
KTINSIEEHFNQWFHYPYVFLNDQPFEEDFKAKVRDVTVGALVEFGTIDEISWNFPSD
VKDTFEFYNAIEDQGDRSILYGNLESYHKMCRFYSGLFYKHPLVQKYEWYWRLEPDVE
FFCDITYDPFLEMLRTNKKYGFTIIIPELYWTVPNLFRHTKSFISQKGVTLGSLWKLF
TKDYDIFESDDPELRDWINYDFQAKAKISEKIAIEQLLKKGDDFQQINDDKEGIMNLI
HKARSRKHIVEDKFFNEEYNLCHFWSNFEIARLSVFDNDIYNSFFQYLEKSGGFWKER
WGDAPVHSIGLSLTLDLDDVHYFRDIGYRHSTIQHCPHNAMGNEEFSYLASDSKFKRK
NAAYDEGREFGCGCRCRCPKKKREIEDSMGFCVNIWVNLLNQQRGHERHVEALNGNEM
EEHIREDYLRQFGN"
gene <579580..>581616
/gene="CRZ1"
/locus_tag="YNL027W"
/gene_synonym="HAL8; TCN1"
/db_xref="GeneID:855704"
mRNA <579580..>581616
/gene="CRZ1"
/locus_tag="YNL027W"
/gene_synonym="HAL8; TCN1"
/product="DNA-binding transcription factor CRZ1"
/transcript_id="NM_001182866.1"
/db_xref="GeneID:855704"
CDS 579580..581616
/gene="CRZ1"
/locus_tag="YNL027W"
/gene_synonym="HAL8; TCN1"
/experiment="EXISTENCE:direct assay:GO:0000987
cis-regulatory region sequence-specific DNA binding
[PMID:11523797]"
/experiment="EXISTENCE:direct assay:GO:0001228 DNA-binding
transcription activator activity, RNA polymerase
II-specific [PMID:20412803|PMID:11523797]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10197980]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:10197980]"
/experiment="EXISTENCE:direct assay:GO:0010765 positive
regulation of sodium ion transport [PMID:11523797]"
/experiment="EXISTENCE:direct assay:GO:0030007
intracellular potassium ion homeostasis [PMID:20412803]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:19111667]"
/experiment="EXISTENCE:direct assay:GO:0045944 positive
regulation of transcription by RNA polymerase II
[PMID:11523797|PMID:20412803]"
/experiment="EXISTENCE:direct assay:GO:0071483 cellular
response to blue light [PMID:23335962]"
/experiment="EXISTENCE:genetic interaction:GO:0006031
chitin biosynthetic process [PMID:21343301]"
/experiment="EXISTENCE:genetic interaction:GO:0030007
intracellular potassium ion homeostasis [PMID:20412803]"
/experiment="EXISTENCE:genetic interaction:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:20412803|PMID:25258318|PMID:9407035]"
/experiment="EXISTENCE:genetic interaction:GO:0050801
monoatomic ion homeostasis [PMID:9407035]"
/experiment="EXISTENCE:genetic interaction:GO:0071277
cellular response to calcium ion [PMID:9407035]"
/experiment="EXISTENCE:mutant phenotype:GO:0030007
intracellular potassium ion homeostasis [PMID:20412803]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:11523797|PMID:9407035|PMID:20412803|PMID:9407036|PMI
D:9559673|PMID:15299026]"
/experiment="EXISTENCE:mutant phenotype:GO:0050801
monoatomic ion homeostasis [PMID:9407035]"
/experiment="EXISTENCE:mutant phenotype:GO:0071277
cellular response to calcium ion
[PMID:9407035|PMID:9407036|PMID:11523797]"
/experiment="EXISTENCE:mutant phenotype:GO:0071444
cellular response to pheromone
[PMID:9407035|PMID:9407036]"
/experiment="EXISTENCE:mutant phenotype:GO:0071469
cellular response to alkaline pH
[PMID:11523797|PMID:15299026]"
/experiment="EXISTENCE:mutant phenotype:GO:0071475
cellular hyperosmotic salinity response
[PMID:11523797|PMID:9559673|PMID:9407035]"
/experiment="EXISTENCE:mutant phenotype:GO:2001040
positive regulation of cellular response to drug
[PMID:17056742]"
/note="Transcription factor, activates transcription of
stress response genes; nuclear localization is positively
regulated by calcineurin-mediated dephosphorylation;
rapidly localizes to the nucleus under blue light stress;
can be activated in stochastic pulses of nuclear
localization in response to calcium"
/codon_start=1
/product="DNA-binding transcription factor CRZ1"
/protein_id="NP_014371.1"
/db_xref="GeneID:855704"
/db_xref="SGD:S000004972"
/translation="MSFSNGNMASYMTSSNGEEQSINNKNDIDDNSAYRRNNFRNSSN
SGSHTFQLSDLDLDVDMRMDSANSSEKISKNLSSGIPDSFDSNVNSLLSPSSGSYSAD
LNYQSLYKPDLPQQQLQQQQLQQQQQQQQQQQQQQQKQTPTLKVEQSDTFQWDDILTP
ADNQHRPSLTNQFLSPRSNYDGTTRSSGIDSNYSDTESNYHTPYLYPQDLVSSPAMSH
LTANNDDFDDLLSVASMNSNYLLPVNSHGYKHISNLDELDDLLSLTYSDNNLLSASNN
SDFNNSNNGIINTADTQNSTIAINKSKVGTNQKMLLTIPTSSTPSPSTHAAPVTPIIS
IQEFNEGHFPVKNEDDGTLQLKVRDNESYSATNNNNLLRPDDNDYNNEALSDIDRSFE
DIINGRKLKLKKSRRRSSQTSNNSFTSRRSSRSRSISPDEKAKSISANREKLLEMADL
LPSSENDNNRERYDNDSKTSYNTINSSNFNEDNNNNNLLTSKPKIESGIVNIKNELDD
TSKDLGILLDIDSLGQFEQKVGFKNDDNHENNDNGTFSVKKNDNLEKLDSVTNNRKNP
ANFACDVCGKKFTRPYNLKSHLRTHTNERPFICSICGKAFARQHDRKRHEDLHTGKKR
YVCGGKLKDGKPWGCGKKFARSDALGRHFKTESGRRCITPLYEEARQEKSGQES"
gene <581920..>583374
/gene="SAM50"
/locus_tag="YNL026W"
/gene_synonym="OMP85; TOB55"
/db_xref="GeneID:855705"
mRNA <581920..>583374
/gene="SAM50"
/locus_tag="YNL026W"
/gene_synonym="OMP85; TOB55"
/product="SAM complex subunit SAM50"
/transcript_id="NM_001182865.1"
/db_xref="GeneID:855705"
CDS 581920..583374
/gene="SAM50"
/locus_tag="YNL026W"
/gene_synonym="OMP85; TOB55"
/experiment="EXISTENCE:direct assay:GO:0001401 SAM complex
[PMID:14570913]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:16823961|PMID:14690591|PMID:14576278|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane
[PMID:14699090|PMID:16407407]"
/experiment="EXISTENCE:direct assay:GO:0008320 protein
transmembrane transporter activity [PMID:14685243]"
/experiment="EXISTENCE:mutant phenotype:GO:0045040 protein
insertion into mitochondrial outer membrane
[PMID:18039934|PMID:14570913]"
/experiment="EXISTENCE:mutant phenotype:GO:0065003
protein-containing complex assembly
[PMID:14685243|PMID:14570913]"
/note="Component of the Sorting and Assembly Machinery
(SAM) complex; the SAM (or TOB) complex is located in the
mitochondrial outer membrane; the complex binds precursors
of beta-barrel proteins and facilitates their outer
membrane insertion; homologous to bacterial Omp85"
/codon_start=1
/product="SAM complex subunit SAM50"
/protein_id="NP_014372.1"
/db_xref="GeneID:855705"
/db_xref="SGD:S000004971"
/translation="MTSSSGVDNEISLDSPMPIFNESSTLKPIRVAGVVTTGTDHIDP
SVLQAYLDDTIMKSITLGQLVKNADVLNKRLCQHHIALNAKQSFHFQGNTYISDEKET
HDVVPLMEVVSQLDILPPKTFTAKTGTNFGNDNDAEAYLQFEKLIDKKYLKLPTRVNL
EILRGTKIHSSFLFNSYSSLSPQSILNLKVFSQFYNWNTNKGLDIGQRGARLSLRYEP
LFLHKLLHNPHSNESPTLFHEWFLETCWRSTKICSQGTSAPYMYSGTMLSQAGDQLRT
ILGHTFVLDKRDHIMCPTKGSMLKWSNELSPGKHLKTQLELNSVKSWMNDDFITFSTT
IKTGYLKNLSSQQSLPVHICDKFQSGGPSDIRGFQTFGLGPRDLYDAVGGDAFVSYGL
SVFSRLPWKKVEKSNFRLHWFFNGGKLVNHDNTSLGNCIGQLSKEHSTSTGIGLVLRH
PMARFELNFTLPITAHENDLIRKGFQFGLGLAFL"
gene complement(<584320..>585291)
/gene="SSN8"
/locus_tag="YNL025C"
/gene_synonym="CNC1; GIG3; NUT9; RYE2; SRB11; UME3"
/db_xref="GeneID:855706"
mRNA complement(<584320..>585291)
/gene="SSN8"
/locus_tag="YNL025C"
/gene_synonym="CNC1; GIG3; NUT9; RYE2; SRB11; UME3"
/product="cyclin-dependent protein serine/threonine kinase
regulator SSN8"
/transcript_id="NM_001182864.3"
/db_xref="GeneID:855706"
CDS complement(584320..585291)
/gene="SSN8"
/locus_tag="YNL025C"
/gene_synonym="CNC1; GIG3; NUT9; RYE2; SRB11; UME3"
/experiment="EXISTENCE:direct assay:GO:0000979 RNA
polymerase II core promoter sequence-specific DNA binding
[PMID:23447536]"
/experiment="EXISTENCE:direct assay:GO:0016538
cyclin-dependent protein serine/threonine kinase regulator
activity [PMID:7877695]"
/experiment="EXISTENCE:direct assay:GO:0016592 mediator
complex [PMID:11383511|PMID:12200444]"
/experiment="EXISTENCE:direct assay:GO:0034605 cellular
response to heat [PMID:23447536]"
/experiment="EXISTENCE:direct assay:GO:0045944 positive
regulation of transcription by RNA polymerase II
[PMID:14749387]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:16109375]"
/experiment="EXISTENCE:mutant phenotype:GO:0000411
positive regulation of transcription by galactose
[PMID:15601835]"
/experiment="EXISTENCE:mutant phenotype:GO:0016538
cyclin-dependent protein serine/threonine kinase regulator
activity [PMID:7732022]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:12455972]"
/experiment="EXISTENCE:mutant phenotype:GO:0051321 meiotic
cell cycle [PMID:12455972]"
/note="Cyclin-like component of RNA polymerase II
holoenzyme; involved in phosphorylation of RNAPII
C-terminal domain; forms kinase-cyclin pair in RNAPII
holoenzyme with Ssn3p; needed for entry into and execution
of meiotic program; involved in glucose repression,
telomere maintenance; degraded in response to nitrogen
starvation, and also to oxidative stress, but in different
cellular compartments, and with different cellular
outcomes; 35% identical to human cyclin C"
/codon_start=1
/product="cyclin-dependent protein serine/threonine kinase
regulator SSN8"
/protein_id="NP_014373.3"
/db_xref="GeneID:855706"
/db_xref="SGD:S000004970"
/translation="MSGSFWTSTQRHHWQYTKASLAKERQKLWLLECQLFPQGLNIVM
DSKQNGIEQSITKNIPITHRDLHYDKDYNLRIYCYFLIMKLGRRLNIRQYALATAHIY
LSRFLIKASVREINLYMLVTTCVYLACKVEECPQYIRTLVSEARTLWPEFIPPDPTKV
TEFEFYLLEELESYLIVHHPYQSLKQIVQVLKQPPFQITLSSDDLQNCWSLINDSYIN
DVHLLYPPHIIAVACLFITISIHGKPTKGSSLASAASEAIRDPKNSSSPVQIAFNRFM
AESLVDLEEVMDTIQEQITLYDHWDKYHEQWIKFLLHTLYLRPASAI"
gene 585587..585926
/gene="NME1"
/locus_tag="YNCN0013W"
/db_xref="GeneID:9164967"
ncRNA 585587..585926
/ncRNA_class="RNase_MRP_RNA"
/gene="NME1"
/locus_tag="YNCN0013W"
/product="NME1"
/experiment="EXISTENCE:direct assay:GO:0000172
ribonuclease MRP complex [PMID:1398074]"
/experiment="EXISTENCE:direct assay:GO:0006364 rRNA
processing [PMID:8602511]"
/experiment="EXISTENCE:mutant phenotype:GO:0000171
ribonuclease MRP activity
[PMID:8247008|PMID:8602511|PMID:8290578]"
/experiment="EXISTENCE:mutant phenotype:GO:0006364 rRNA
processing [PMID:8247008|PMID:8290578]"
/experiment="EXISTENCE:mutant phenotype:GO:0006397 mRNA
processing [PMID:22977255]"
/experiment="EXISTENCE:mutant phenotype:GO:0045787
positive regulation of cell cycle [PMID:12136008]"
/note="RNA component of RNase MRP; RNase MRP cleaves
pre-rRNA and has a role in cell cycle-regulated
degradation of daughter cell-specific mRNAs; human
ortholog is implicated in cartilage-hair hypoplasia (CHH)"
/transcript_id="NR_132251.1"
/db_xref="GeneID:9164967"
/db_xref="SGD:S000007436"
gene 586090..586175
/gene="SNR66"
/locus_tag="YNCN0014W"
/db_xref="GeneID:9164968"
ncRNA 586090..586175
/ncRNA_class="snoRNA"
/gene="SNR66"
/locus_tag="YNCN0014W"
/product="SNR66"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:10024243]"
/experiment="EXISTENCE:curator inference:GO:0031428 box
C/D methylation guide snoRNP complex [PMID:10024243]"
/experiment="EXISTENCE:mutant phenotype:GO:0030562 rRNA
2'-O-ribose methylation guide activity [PMID:10024243]"
/experiment="EXISTENCE:mutant phenotype:GO:0031167 rRNA
methylation [PMID:10024243]"
/note="C/D box small nucleolar RNA (snoRNA); guides
2'-O-methylation of large subunit (LSU) rRNA at position
U2417"
/transcript_id="NR_132252.1"
/db_xref="GeneID:9164968"
/db_xref="SGD:S000006457"
gene complement(<586602..>586820)
/gene="KSH1"
/locus_tag="YNL024C-A"
/db_xref="GeneID:1466516"
mRNA complement(<586602..>586820)
/gene="KSH1"
/locus_tag="YNL024C-A"
/product="Ksh1p"
/transcript_id="NM_001184619.3"
/db_xref="GeneID:1466516"
CDS complement(586602..586820)
/gene="KSH1"
/locus_tag="YNL024C-A"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:19942856]"
/note="Essential protein suggested to function early in
the secretory pathway; inviability is suppressed by
overexpression of Golgi protein Tvp23p; SWAT-GFP and
mCherry fusion proteins localize to the endoplasmic
reticulum and cytosol respectively; ortholog of human
Kish"
/codon_start=1
/product="Ksh1p"
/protein_id="NP_878158.3"
/db_xref="GeneID:1466516"
/db_xref="SGD:S000028698"
/translation="MSALFNFRSLLQVILLLICSCSYVHGQWPSLLDRYKNHEVLGAF
WKMARVGERASPYVSLACILMAISQFNS"
gene complement(<587107..>587847)
/gene="EFM6"
/locus_tag="YNL024C"
/db_xref="GeneID:855707"
mRNA complement(<587107..>587847)
/gene="EFM6"
/locus_tag="YNL024C"
/product="putative protein-lysine N-methyltransferase"
/transcript_id="NM_001182863.1"
/db_xref="GeneID:855707"
CDS complement(587107..587847)
/gene="EFM6"
/locus_tag="YNL024C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="Putative S-adenosylmethionine-dependent lysine
methyltransferase; responsible for modifying Lys-390 in
translational elongation factor EF-1 alpha (eEF1A); has
seven beta-strand methyltransferase motif; green
fluorescent protein (GFP)-fusion protein localizes to the
cytoplasm"
/codon_start=1
/product="putative protein-lysine N-methyltransferase"
/protein_id="NP_014374.1"
/db_xref="GeneID:855707"
/db_xref="SGD:S000004969"
/translation="MESIFGGFGDLVVPRPKEHLGQTDLSFGGKLLPALKICEDGGES
GCGGKVWIAGELLCEYILEKSVDHLLSKTVNGTKQFKKVLELGSGTGLVGLCVGLLEK
NTFHDGTKVYVTDIDKLIPLLKRNIELDEVQYEVLARELWWGEPLSADFSPQEGAMQA
NNVDLVLAADCVYLEEAFPLLEKTLLDLTHCINPPVILMAYKKRRKADKHFFNKIKRN
FDVLEITDFSKFEHYLKERTHLFQLIRK"
gene complement(<588263..>591160)
/gene="FAP1"
/locus_tag="YNL023C"
/db_xref="GeneID:855708"
mRNA complement(<588263..>591160)
/gene="FAP1"
/locus_tag="YNL023C"
/product="Fap1p"
/transcript_id="NM_001182862.1"
/db_xref="GeneID:855708"
CDS complement(588263..591160)
/gene="FAP1"
/locus_tag="YNL023C"
/experiment="EXISTENCE:direct assay:GO:0005515 protein
binding [PMID:36113412]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:36113412|PMID:10998178]"
/experiment="EXISTENCE:direct assay:GO:0070651
nonfunctional rRNA decay [PMID:36113412]"
/note="Cytoplasmic RING-finger E3 Ub-ligase and sensor of
stalled ribosomes; binds to Fpr1p and Smu2p, and triggers
K63-linked polyubiquitination of monoubiquitinated 40S
subunit Rps3p, from decoding-defective ribosomes, followed
by dissociation of the 80S ribosomes and non-functional
rRNA decay (NRD) of 18S rRNA; confers rapamycin resistance
by competing with rapamycin for Fpr1p binding; accumulates
in the nucleus in rapamycin-treated cells; ortholog of D.
melanogaster shuttle craft and human NFX1"
/codon_start=1
/product="Fap1p"
/protein_id="NP_014375.1"
/db_xref="GeneID:855708"
/db_xref="SGD:S000004968"
/translation="MTEHESLGLEQNQDGGDTYRHHNLSDGCISSVEDANEQPSSYEE
ESDDDMQYYERAIQEISSGDSYVCMICTVEMDYTCQMFACKRCYRVFDYGCIREWALK
STEKTVDRIWKCPNCYYVSKRVPVKNRPTCWCGKVVNPDPNPLDPNSCGQTCNASTCM
HGCSKICHLGPHPECTRMVEIMCHCGKHSKSIFCYQSKVMKKNFNCQEVCGLPLSCSI
HTCKKKCHPGLCGPCPEMIISKDSPKKQIKCYCGNHTRANIKCSETKFPKSGKSSKDE
NGNRWIGVFACADNRVVDYSCRKHSFIESCISPPTINGEKACPFLPSSLKTCPCGRTA
LEELTKPRKHCDDPIPTCDSRCGKPLKCGKHSCPFTCHDKACMEPCLQIDSVKCACEQ
STFSVPCGFQGRPRCNIKCESLMSCRRHRCTDRCCSGRPSAIRRKKNLFRTQDLLDES
LVEAKHICLKPCNLTLSCGIHKCQRKCHPGKCPPCLESDSNDLVCPCGNTVVPAPVRC
GTKLPTCNHPCIKVVRGESTCGHKPMPHTCHSLDVSCPPCTETVFKPCKCGKKTKVRT
VCFQTDVSCGIKCGIPLSYCYHTCQKTCHLPGNCQKVCKQTCGQKRLNCNHECPKPCH
GKTECPDLPCATLVKIYCKCGRIKKSVTCGAKSDRVSVTESSVLDCNEECEALKRLKE
LKEAFGIKEETNNFTSNELDALKKLVSVATTFEELQLPFTEAALSVYSKQERWCSQIE
AILNKLMDDKTRSSLHFKPMRPPQRHFIRELAKAYGLYSESQDREPMRSVFIKKEDNG
ASNKPVLSLAEAYPLYESFKQLQKERKAQEFQARTTAKLINFEVQDTEPKVEVAKKNG
FLVQNLVAGNTAEDLRRFFEPHLKHTLVVNPQYLILDDGKTALVYPENYETASVNTER
DMELLVGHFDFMAKEAFLADSISLCSTEEELERRLDTPVIQEDSPVMDNNT"
gene complement(<591427..>592899)
/gene="RCM1"
/locus_tag="YNL022C"
/db_xref="GeneID:855709"
mRNA complement(<591427..>592899)
/gene="RCM1"
/locus_tag="YNL022C"
/product="rRNA (cytosine-C5-)-methyltransferase RCM1"
/transcript_id="NM_001182861.3"
/db_xref="GeneID:855709"
CDS complement(591427..592899)
/gene="RCM1"
/locus_tag="YNL022C"
/EC_number="2.1.1.311"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10649453]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:23913415]"
/experiment="EXISTENCE:direct assay:GO:0009383 rRNA
(cytosine-C5-)-methyltransferase activity [PMID:23913415]"
/experiment="EXISTENCE:direct assay:GO:0070475 rRNA base
methylation [PMID:23913415]"
/experiment="EXISTENCE:genetic interaction:GO:0009383 rRNA
(cytosine-C5-)-methyltransferase activity [PMID:23913415]"
/experiment="EXISTENCE:genetic interaction:GO:0070475 rRNA
base methylation [PMID:23913415]"
/experiment="EXISTENCE:mutant phenotype:GO:0009383 rRNA
(cytosine-C5-)-methyltransferase activity [PMID:23913415]"
/experiment="EXISTENCE:mutant phenotype:GO:0070475 rRNA
base methylation [PMID:23913415]"
/note="rRNA m5C methyltransferase; methylates cytosine at
position 2278 of 25S rRNA while Nop2p methylates cytosine
at position 2870; contains seven beta-strand
methyltransferase motif; localized to the nucleolus;
interacts with Trm112p; homolog of NSUN5A, a human gene
which is deleted in Williams-Beuren Syndrome"
/codon_start=1
/product="rRNA (cytosine-C5-)-methyltransferase RCM1"
/protein_id="NP_014376.3"
/db_xref="GeneID:855709"
/db_xref="SGD:S000004967"
/translation="MNFYRDATWVLEDIEKEAAKERISGSMQTLVLKSCKRYKLKSNP
KHIYAVLDSCWKYKPYLEKVMKKAHILEDIPKKKGKPLFSRLTLLLLCHDLLLSKQKR
IQMGKHPIKDYVLKFKSPLHSEMVKLKLKLKVRELSELVLSEDISNDLPPVRWIRINP
LKCHPNGETEPVLAELRKKFTLKVDKWSELVPGSIYYDEFIPNLFGIHPSDKITAHEL
YKHGKIIIQDRASCFPAHILNPGPSDIVIDSCSAPGNKTTHTASYIYPEPPKDNNTRI
YAFEKDPERAKVLQKMIKIAGCSPNISVNVGDFTKLATPEKYKDVTCFIVDPSCSGSG
IFGRKFFDSFNRRKIDDKDDDGGIVPDEQEEFIAKEELQTRLAKLSSFQFQMVKHAMS
FPAAKKIVYSTCSIHAEENERVVIDLLLDKSVREWGWKVAPKREVIPSWPRRGKVEEF
EEVFRDGVTYDPQQLAEGCIRALPKSDGGIGFFAVCFERD"
gene <593227..>595347
/gene="HDA1"
/locus_tag="YNL021W"
/db_xref="GeneID:855710"
mRNA <593227..>595347
/gene="HDA1"
/locus_tag="YNL021W"
/product="histone deacetylase HDA1"
/transcript_id="NM_001182860.1"
/db_xref="GeneID:855710"
CDS 593227..595347
/gene="HDA1"
/locus_tag="YNL021W"
/EC_number="3.5.1.98"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:16415367]"
/experiment="EXISTENCE:direct assay:GO:0004407 histone
deacetylase activity [PMID:19573535]"
/experiment="EXISTENCE:direct assay:GO:0070823 HDA1
complex [PMID:11287668|PMID:8663039|PMID:8962081]"
/experiment="EXISTENCE:genetic interaction:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:17974563]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:17121596|PMID:11172717]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:17706600]"
/experiment="EXISTENCE:physical interaction:GO:0070823
HDA1 complex [PMID:11287668]"
/note="Catalytic subunit of class II HDA1 histone
deacetylase complex; role in azole resistance via Hsp90p,
and in heat shock response; Hda1p interacts with
Hda2p-Hda3p subcomplex to form an active tetramer;
deletion increases histone H2B, H3 and H4 acetylation; the
HDA1 histone deacetylase complex regulates aging through
trehalose metabolism"
/codon_start=1
/product="histone deacetylase HDA1"
/protein_id="NP_014377.1"
/db_xref="GeneID:855710"
/db_xref="SGD:S000004966"
/translation="MDSVMVKKEVLENPDHDLKRKLEENKEEENSLSTTSKSKRQVIV
PVCMPKIHYSPLKTGLCYDVRMRYHAKIFTSYFEYIDPHPEDPRRIYRIYKILAENGL
INDPTLSGVDDLGDLMLKIPVRAATSEEILEVHTKEHLEFIESTEKMSREELLKETEK
GDSVYFNNDSYASARLPCGGAIEACKAVVEGRVKNSLAVVRPPGHHAEPQAAGGFCLF
SNVAVAAKNILKNYPESVRRIMILDWDIHHGNGTQKSFYQDDQVLYVSLHRFEMGKYY
PGTIQGQYDQTGEGKGEGFNCNITWPVGGVGDAEYMWAFEQVVMPMGREFKPDLVIIS
SGFDAADGDTIGQCHVTPSCYGHMTHMLKSLARGNLCVVLEGGYNLDAIARSALSVAK
VLIGEPPDELPDPLSDPKPEVIEMIDKVIRLQSKYWNCFRRRHANSGCNFNEPINDSI
ISKNFPLQKAIRQQQQHYLSDEFNFVTLPLVSMDLPDNTVLCTPNISESNTIIIVVHD
TSDIWAKRNVISGTIDLSSSVIIDNSLDFIKWGLDRKYGIIDVNIPLTLFEPDNYSGM
ITSQEVLIYLWDNYIKYFPSVAKIAFIGIGDSYSGIVHLLGHRDTRAVTKTVINFLGD
KQLKPLVPLVDETLSEWYFKNSLIFSNNSHQCWKENESRKPRKKFGRVLRCDTDGLNN
IIEERFEEATDFILDSFEEWSDEE"
gene complement(<595623..>597539)
/gene="ARK1"
/locus_tag="YNL020C"
/db_xref="GeneID:855711"
mRNA complement(<595623..>597539)
/gene="ARK1"
/locus_tag="YNL020C"
/product="serine/threonine protein kinase ARK1"
/transcript_id="NM_001182859.1"
/db_xref="GeneID:855711"
CDS complement(595623..597539)
/gene="ARK1"
/locus_tag="YNL020C"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0004674 protein
serine/threonine kinase activity [PMID:17978096]"
/experiment="EXISTENCE:direct assay:GO:0030479 actin
cortical patch [PMID:10087264]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807]"
/experiment="EXISTENCE:genetic interaction:GO:2000369
regulation of clathrin-dependent endocytosis
[PMID:12956961]"
/experiment="EXISTENCE:mutant phenotype:GO:0004674 protein
serine/threonine kinase activity [PMID:10087264]"
/experiment="EXISTENCE:mutant phenotype:GO:0007015 actin
filament organization [PMID:10087264]"
/experiment="EXISTENCE:mutant phenotype:GO:2000369
regulation of clathrin-dependent endocytosis
[PMID:12956961]"
/note="Ser/Thr protein kinase; phosphorylates
Pan1p-Sla1p-End3p protein complex subunits, Pan1p and
Sla1p; involved in regulation of the cortical actin
cytoskeleton and endocytosis; functional overlap with
PRK1"
/codon_start=1
/product="serine/threonine protein kinase ARK1"
/protein_id="NP_014378.1"
/db_xref="GeneID:855711"
/db_xref="SGD:S000004965"
/translation="MNQPQIGTYNVGTQLTVGSHQVEIIKYLTSGGFAQVYSALINPP
DPHSNSSVACLKRVIVPDKPSLNTLRAEVDAMRLLKNNRYVVSYIDSHAAKAMLHNGS
YEVFVLMEYCERGGLIDFMNTRLQNRLHEFEILQIMSQVTQGVAAMHALQPPLIHRDI
KIENVLISANNEYKLCDFGSVCGIIRPPRNSQELSYVQQDILKNTTAQYRSPEMIDTF
RGLPIDEKSDIWALGIFLYKLCYYTTPFEKGGDLAILSGKFEFPLYPNYSEQLKGLIR
DILVQDPRHRPNVYQLLKRISIMQNVPCPINDIQVVQAPSSHLNLTELHQLSATQNIL
SLNSPTTMENTMPNATFQISMADNTTTAQMHPNRKPSQIAYDASFSNSAKGSQPLFDK
SQNMYHALDPPLVEPLASSVSNNDNELKANSATKLKQAIVSEAHTFRQNNSIDFPLQN
IIPQYEDSSSSSDESYSGDVDELKKTRSLGSYSTRGNIKKNQSVKESLTSSSLPGTSF
TPTSTKVNLKHENSPFKSTFVNTIDNSKDDLNKPSYEDLDVSKQNLKNSIQQRMIDKL
NSSEESFNARKMSKVKLHEKGEIDKPTMLKSSGPISKDKKTKPTPPPKPSHLKPKPPP
KPLLLAGRKLSLDK"
gene complement(<598376..>599230)
/locus_tag="YNL019C"
/db_xref="GeneID:855712"
mRNA complement(<598376..>599230)
/locus_tag="YNL019C"
/product="uncharacterized protein"
/transcript_id="NM_001182858.3"
/db_xref="GeneID:855712"
CDS complement(598376..599230)
/locus_tag="YNL019C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:24390141]"
/note="hypothetical protein; expression induced during
heat stress; YNL019C has a paralog, YNL033W, that arose
from a segmental duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014379.3"
/db_xref="GeneID:855712"
/db_xref="SGD:S000004964"
/translation="MLYSRESRTTVLFLALVTSLTVLCHSVDVTTVFTTSTITEITTV
TAAPQPQNKAETALNTATNIIQTMQFLFNCAPFKWKGPLKITSCALNFIVLLLTAWGY
LLKYLQENKLNSDADMEKMVGLGFGEMVGRIFGKGVGKAFTKMDITQKLVYPFEGSNR
QKCLLMTVGENSIVPFHDLSTEICFDQYTLDSLSHHNHGSISILDAGSVSALGFADIS
SKMPSVSELYTLFGDYTIEVLGGITKLASTLNREDWQGERNGFAVLSRDRPNQTLLSV
HMYSSSLL"
gene complement(<599936..>601774)
/locus_tag="YNL018C"
/db_xref="GeneID:855713"
mRNA complement(<599936..>601774)
/locus_tag="YNL018C"
/product="uncharacterized protein"
/transcript_id="NM_001182857.3"
/db_xref="GeneID:855713"
CDS complement(599936..601774)
/locus_tag="YNL018C"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:24390141]"
/experiment="EXISTENCE:mutant phenotype:GO:0043935 sexual
sporulation resulting in formation of a cellular spore
[PMID:36441813]"
/note="Protein required for sporulation; induced during
meiosis and sporulation; gene is a member of Centroid, a
centromere-linked gene family; YNL018C has a paralog,
YNL034W, that arose from a segmental duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014380.3"
/db_xref="GeneID:855713"
/db_xref="SGD:S000004963"
/translation="MSTSFQEFKAFCNKVGLDFQWLNLQSSKSVPENGSSEGFSAVSD
TVQENIRPATEPLNVNQSKDPVSNFFYDVKNAPLWNVYKRNHSGHSSAEACSGVSSRQ
APKNIPEAMVKETVLSNHDNVTIINELLPTSSAMHQEESTAMTTSYLLSHSVNDTCNV
MFSSSSHNRAMLPSSLVQRNNATTSPTTDSASENNESVPSLTSSVSTSSSVYSSWNPP
HSPHISSFPDGNFASLNAEVTCFDFRRTKDSRTKETNESIIPTEIYCPTNSTDHHKHY
PSRKSKQDACAPAPCNQNISCSVVSTAEFSQSNHTLTTVVPSYMQQYLDRPQNWFESK
MGKYCPLFLRSTKNIDYDSLEFKFERKMIAVQYLLLDEQSEPRRYYNPSNKSIPFWKR
PFNFDTMPSYDQLMEEAECRFYSYQYKYEGFQRIEPYSISCPWKNTQREIDLVLDHIH
FSLDVGEKKSLNRKGNITLDTLDSKVDPNIQIKPYQIFPSNNLVYEGLPHPAEQSLIL
SPDTSLIERAFQALIDICKESIPSSNDCSTRNNNSAPQLTVPEPSKPCRLLLVRESRT
ATELKTNKKLWLHSQRRNIEVTVPMHPSERGTKSWLRKWLSTFVHQ"
gene 602312..602385
/locus_tag="YNCN0015W"
/db_xref="GeneID:855714"
tRNA 602312..602385
/locus_tag="YNCN0015W"
/product="tRNA-Ile"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Isoleucine tRNA (tRNA-Ile), predicted by
tRNAscan-SE analysis"
/db_xref="GeneID:855714"
/db_xref="SGD:S000006610"
gene <602907..>604268
/gene="PUB1"
/locus_tag="YNL016W"
/gene_synonym="RNP1"
/db_xref="GeneID:855716"
mRNA <602907..>604268
/gene="PUB1"
/locus_tag="YNL016W"
/gene_synonym="RNP1"
/product="Pub1p"
/transcript_id="NM_001182855.1"
/db_xref="GeneID:855716"
CDS 602907..604268
/gene="PUB1"
/locus_tag="YNL016W"
/gene_synonym="RNP1"
/experiment="EXISTENCE:direct assay:GO:0000184
nuclear-transcribed mRNA catabolic process,
nonsense-mediated decay [PMID:10892745]"
/experiment="EXISTENCE:direct assay:GO:0000932 P-body
[PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding
[PMID:23222640|PMID:15964806|PMID:8413212|PMID:8413213]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:8413212]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:8413212|PMID:8413213]"
/experiment="EXISTENCE:direct assay:GO:0008266 poly(U) RNA
binding [PMID:8413213]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule
[PMID:24291094|PMID:26777405|PMID:18981231]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:28467675]"
/experiment="EXISTENCE:mutant phenotype:GO:0003729 mRNA
binding [PMID:8413212|PMID:8413213]"
/experiment="EXISTENCE:mutant phenotype:GO:0006415
translational termination [PMID:28467675]"
/experiment="EXISTENCE:mutant phenotype:GO:0034063 stress
granule assembly [PMID:18981231]"
/experiment="EXISTENCE:mutant phenotype:GO:0042149
cellular response to glucose starvation [PMID:18981231]"
/experiment="EXISTENCE:mutant phenotype:GO:0043488
regulation of mRNA stability [PMID:15964806]"
/experiment="EXISTENCE:physical interaction:GO:0006415
translational termination [PMID:28467675]"
/note="Poly (A)+ RNA-binding protein; abundant
mRNP-component protein that binds cell wall mRNAs;
required for stability of many mRNAs; component of glucose
deprivation induced stress granules, involved in
P-body-dependent granule assembly; implicated in
regulation of translation; carries Q/N-rich domain at C-
terminus, identified as candidate prion; ribosome-bound
Pub1p modulates stop codon decoding during translation
termination"
/codon_start=1
/product="Pub1p"
/protein_id="NP_014382.1"
/db_xref="GeneID:855716"
/db_xref="SGD:S000004961"
/translation="MSENNEEQHQQQQQQQPVAVETPSAVEAPASADPSSEQSVAVEG
NSEQAEDNQGENDPSVVPANAITGGRETSDRVLYVGNLDKAITEDILKQYFQVGGPIA
NIKIMIDKNNKNVNYAFVEYHQSHDANIALQTLNGKQIENNIVKINWAFQSQQSSSDD
TFNLFVGDLNVNVDDETLRNAFKDFPSYLSGHVMWDMQTGSSRGYGFVSFTSQDDAQN
AMDSMQGQDLNGRPLRINWAAKRDNNNNNNYQQRRNYGNNNRGGFRQYNSNNNNNMNM
GMNMNMNMNMNNSRGMPPSSMGMPIGAMPLPSQGQPQQSQTIGLPPQVNPQAVDHIIR
SAPPRVTTAYIGNIPHFATEADLIPLFQNFGFILDFKHYPEKGCCFIKYDTHEQAAVC
IVALANFPFQGRNLRTGWGKERSNFMPQQQQQGGQPLIMNDQQQPVMSEQQQQQQQQQ
QQQ"
gene <605384..>605611
/gene="PBI2"
/locus_tag="YNL015W"
/gene_synonym="LMA1"
/db_xref="GeneID:855717"
mRNA <605384..>605611
/gene="PBI2"
/locus_tag="YNL015W"
/gene_synonym="LMA1"
/product="Pbi2p"
/transcript_id="NM_001182854.1"
/db_xref="GeneID:855717"
CDS 605384..605611
/gene="PBI2"
/locus_tag="YNL015W"
/gene_synonym="LMA1"
/experiment="EXISTENCE:direct assay:GO:0004866
endopeptidase inhibitor activity [PMID:2015812]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762|PMID:9159115]"
/experiment="EXISTENCE:direct assay:GO:0042144 vacuole
fusion, non-autophagic [PMID:9159115]"
/experiment="EXISTENCE:mutant phenotype:GO:0030162
regulation of proteolysis [PMID:2015812]"
/note="Cytosolic inhibitor of vacuolar proteinase B
(PRB1); required for efficient vacuole inheritance; with
thioredoxin forms protein complex LMA1, which assists in
priming SNARE molecules and promotes vacuole fusion;
protein abundance increases in response to DNA replication
stress"
/codon_start=1
/product="Pbi2p"
/protein_id="NP_014383.1"
/db_xref="GeneID:855717"
/db_xref="SGD:S000004960"
/translation="MTKNFIVTLKKNTPDVEAKKFLDSVHHAGGSIVHEFDIIKGYTI
KVPDVLHLNKLKEKHNDVIENVEEDKEVHTN"
gene <606319..>609453
/gene="HEF3"
/locus_tag="YNL014W"
/gene_synonym="ZRG7"
/db_xref="GeneID:855718"
mRNA <606319..>609453
/gene="HEF3"
/locus_tag="YNL014W"
/gene_synonym="ZRG7"
/product="translation elongation factor EF-3"
/transcript_id="NM_001182853.3"
/db_xref="GeneID:855718"
CDS 606319..609453
/gene="HEF3"
/locus_tag="YNL014W"
/gene_synonym="ZRG7"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:9544245]"
/experiment="EXISTENCE:genetic interaction:GO:0003746
translation elongation factor activity [PMID:9544245]"
/experiment="EXISTENCE:genetic interaction:GO:0006414
translational elongation [PMID:9544245]"
/experiment="EXISTENCE:physical interaction:GO:0022626
cytosolic ribosome [PMID:9544245]"
/note="Translational elongation factor EF-3; member of the
ABC superfamily; stimulates EF-1 alpha-dependent binding
of aminoacyl-tRNA by the ribosome; normally expressed in
zinc deficient cells; HEF3 has a paralog, YEF3, that arose
from the whole genome duplication"
/codon_start=1
/product="translation elongation factor EF-3"
/protein_id="NP_014384.3"
/db_xref="GeneID:855718"
/db_xref="SGD:S000004959"
/translation="MSDSQQSITVLEELFRKLETATSETREGISSELSSFLNGNIIEH
DVPEVFFDEFQKAIQSKQKALNTLGAVAYIANETNLSPSVEPYIVATVPSVCSKAGSK
DNDVQLAATKALKAIASAVNPVAVKALLPHLIHSLETSNKWKEKVAVLEVISVLVDAA
KEQIALRMPELIPVLSESMWDTKKGVKEAATTTITKATETVDNKDIERFIPKLIECIA
NPNEVPETVHLLGATTFVAEVTPATLSIMVPLLSRGLAERETSIKRKAAVIIDNMCKL
VEDPQVVAPFLGKLLPGLKNNFATIADPEAREVTLKALKTLRRVGNVGEDDVLPEISH
AGDVSTTLGVIKELLEPEKVAPRFTIVVEYIAAIAANLIDERIIDQQTWFTHVTPYMT
IFLHEKTAKEILDDFRKRAVDNIPVGPNFQDEEDEGEDLCNCEFSLAYGAKILLNKTQ
LRLKRGRRYGLCGPNGAGKSTLMRSIANGQVDGFPTQDECRTVYVEHDIDNTHSDMSV
LDFVYSGNVGTKDVITSKLKEFGFSDEMIEMPIASLSGGWKMKLALARAVLKDADILL
LDEPTNHLDTVNVEWLVNYLNTCGITSVIVSHDSGFLDKVCQYIIHYEGLKLRKYKGN
LSEFVQKCPTAQSYYELGASDLEFQFPTPGYLEGVKTKQKAIVKVSNMTFQYPGTTKP
QVSDVTFQCSLSSRIAVIGPNGAGKSTLINVLTGELLPTSGEVYTHENCRIAYIKQHA
FAHIESHLDKTPSEYIQWRFQTGEDRETMDRANRQINENDAEAMNKIFKIEGTPRRVA
GIHSRRKFKNTYEYECSFLLGENIGMKSERWVPMMSVDNAWLPRGELIESHSKMVAEI
DMKEALASGQFRALTRKEIELHCAMLGLDSELVSHSRIRGLSGGQKVKLVLAACTWQR
PHLIVLDEPTNYLDRDSLGALSKALKAFEGGVIIITHSAEFTKNLTDEVWAVKDGKMT
PSGHNWVAGQGAGPRIEKKEEEGDKFDAMGNKINSGKKKSKLSSAELRKKKKERMKKK
KEMGDEYVSSDEDF"
rep_origin 609520..609607
/note="ARS1424; Autonomously Replicating Sequence"
/db_xref="SGD:S000118391"
gene <609686..>611665
/gene="SPO1"
/locus_tag="YNL012W"
/db_xref="GeneID:855720"
mRNA join(<609686..609790,609875..>611665)
/gene="SPO1"
/locus_tag="YNL012W"
/product="putative carboxylic ester hydrolase"
/transcript_id="NM_001182851.1"
/db_xref="GeneID:855720"
CDS join(609686..609790,609875..611665)
/gene="SPO1"
/locus_tag="YNL012W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:18756268]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:10855497]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:17179081]"
/experiment="EXISTENCE:genetic interaction:GO:0032120
ascospore-type prospore membrane formation
[PMID:22611022]"
/experiment="EXISTENCE:genetic interaction:GO:0070583
spore membrane bending pathway [PMID:18756268]"
/experiment="EXISTENCE:mutant phenotype:GO:0007127 meiosis
I [PMID:10855497]"
/experiment="EXISTENCE:mutant phenotype:GO:0030474 spindle
pole body duplication [PMID:10855497]"
/experiment="EXISTENCE:mutant phenotype:GO:0032120
ascospore-type prospore membrane formation
[PMID:26605945|PMID:22611022]"
/experiment="EXISTENCE:mutant phenotype:GO:0070583 spore
membrane bending pathway [PMID:18756268]"
/note="Meiosis-specific prospore protein; required for
meiotic spindle pole body duplication and separation;
required to produce bending force necessary for proper
prospore membrane assembly during sporulation; has
similarity to phospholipase B"
/codon_start=1
/product="putative carboxylic ester hydrolase"
/protein_id="NP_014386.2"
/db_xref="GeneID:855720"
/db_xref="SGD:S000004957"
/translation="MQKLLFVFSVLLTVVLATAPFQVQCPSSPLIREAKHELCPEETL
YLKKKKIKTKNKLIQFLKSLTEAKFSSKFYKRVLKDPPKIGIAISGGGYRSMLVGTGF
ISQMNDYGLFEYSDYIAGLSGGSWILMDLVVQNFEVKSLLQEWDLEEDLLLGIPEFDI
SEEEIVTNAKKEYNDNDLKMKKRQGGSLITSSSNFYEQIEEIMNSIEEIPEDYMITKR
NLNPLARLKKIFFPNNTFTGTDAKIETFKKVLDFYKSLHLKIKPKKMEGFQISFTDYW
GKAIVQRLKKNFDDDPNHSFSFSKLVNSSKKFKECSVPIPIFVANCKNGLLSNVIFEF
TPFEFGSWENILRLFVKLPYLGSKIVSGKAEKCINNFDDLGFITATSSSIFNNVLIFI
WNLASQSSREAMKALNMVMGIFGLGKEEIFSISKDSSRLETDYAVYQPNPFYLYPEKD
NVLTNKNHLYLVDGGEDGENIPLRTLVIPERELDVIFVLDSSSDIDNYPNGSKLKRIF
EKLDEENVHYQFPNNVKTFTHPIVIGCNATKRTGHDSFLPIIIYHANANHGNASNTST
FKITYNQSEVSSMLPTGRGVFSNDYDLYYKNCLGCILTKRTMDRLPRKKKFSPFCLQC
FKDYCYS"
gene complement(<611836..>613170)
/locus_tag="YNL011C"
/db_xref="GeneID:855721"
mRNA complement(<611836..>613170)
/locus_tag="YNL011C"
/product="uncharacterized protein"
/transcript_id="NM_001182850.1"
/db_xref="GeneID:855721"
CDS complement(611836..613170)
/locus_tag="YNL011C"
/note="hypothetical protein; YNL011C is not an essential
gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014387.1"
/db_xref="GeneID:855721"
/db_xref="SGD:S000004956"
/translation="MNVVVCSGGTATNSLTPCFSNISILKGHELTYILPISDNGGSTS
EILRIVGGPAIGDIRSRIVRLLQDEQLVELFGHRLPNDKLLAKKEWNEIVEGSHPIWK
NISIEVKEMCRSFIIHMQAELLKKIKHSNPFQFESASIGNFFLTGARLFLGSLDASIE
LMMRIGRCSPLVHVIPCINTNHTHHISALLTNGEMITGQSQISHPSKSVPKDNSIAHS
AKFIHLLGSYDDHLKILLDDEEEEAEEEYANPIYILPELKNSQLHFDKLDESQNLPAP
VHRILYINPYGEEIKPMGNPRAISKVKKADMVVYSIGSLMTSLLPILILGNLAEVILE
SNNTKKVLLINNKYDREVFGLDGLHYVQMIIDSMSRAIAGYRQSKGVHSENDDFEWQD
FITDIVYLKNGEIEIDETIFEKHSIRCHQIASSDKMESEELEKVLNQIGLKN"
gene <613635..>614360
/gene="PYP1"
/locus_tag="YNL010W"
/db_xref="GeneID:855722"
mRNA <613635..>614360
/gene="PYP1"
/locus_tag="YNL010W"
/product="putative phosphoric monoester hydrolase"
/transcript_id="NM_001182849.3"
/db_xref="GeneID:855722"
CDS 613635..614360
/gene="PYP1"
/locus_tag="YNL010W"
/EC_number="3.1.3.50"
/experiment="EXISTENCE:direct assay:GO:0000121 sn-glycerol
1-phosphatase activity [PMID:26071590]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0043136 sn-glycerol
3-phosphatase activity [PMID:30240188]"
/experiment="EXISTENCE:direct assay:GO:0050286
sorbitol-6-phosphatase activity [PMID:30240188]"
/experiment="EXISTENCE:direct assay:GO:0110130
ribitol-5-phosphatase activity [PMID:30240188]"
/experiment="EXISTENCE:mutant phenotype:GO:0052646 alditol
phosphate metabolic process [PMID:30240188]"
/note="Sugar alcohol phosphatase; polyol phosphatase that
hydrolyzes sorbitol-6-phosphate, ribitol-5-phosphate, and
(D)-glycerol-3-phosphate, maintaining phosphoglucose
isomerase (PGI) activity in the presence of PGI-inhibitory
sugar alcohols; expression correlated with growth rate;
GFP-fusion protein localizes to the cytoplasm and nucleus;
homozygous diploid mutant displays increased glycogen
accumulation; member of the haloacid dehalogenase (HAD)
superfamily"
/codon_start=1
/product="putative phosphoric monoester hydrolase"
/protein_id="NP_014388.3"
/db_xref="GeneID:855722"
/db_xref="SGD:S000004955"
/translation="MVKAVIFTDFDGTVTLEDSNDYLTDTLGFGKEKRLKVFEGVLDD
TKSFRQGFMEMLESIHTPFPECIKILEKKIRLDPGFKDTFEWAQENDVPVIVVSSGMK
PIIKVLLTRLVGQESIHKIDIVSNEVEIDAHDQWKIIYKDESPFGHDKSRSIDAYKKK
FESTLKAGEQRPVYFYCGDGVSDLSAAKECDLLFAKRGKDLVTYCKKQNVPFHEFDTF
KDILASMKQVLAGEKTVAELMEN"
gene <614821..>616083
/gene="IDP3"
/locus_tag="YNL009W"
/db_xref="GeneID:855723"
mRNA <614821..>616083
/gene="IDP3"
/locus_tag="YNL009W"
/product="isocitrate dehydrogenase (NADP(+)) IDP3"
/transcript_id="NM_001182848.3"
/db_xref="GeneID:855723"
CDS 614821..616083
/gene="IDP3"
/locus_tag="YNL009W"
/EC_number="1.1.1.42"
/experiment="EXISTENCE:direct assay:GO:0004450 isocitrate
dehydrogenase (NADP+) activity [PMID:9452501]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276|PMID:15001388]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:9452501]"
/experiment="EXISTENCE:mutant phenotype:GO:0006635 fatty
acid beta-oxidation [PMID:9452501]"
/note="Peroxisomal NADP-dependent isocitrate
dehydrogenase; catalyzes oxidation of isocitrate to
alpha-ketoglutarate with the formation of NADP(H+),
required for growth on unsaturated fatty acids; IDP3 has a
paralog, IDP2, that arose from the whole genome
duplication"
/codon_start=1
/product="isocitrate dehydrogenase (NADP(+)) IDP3"
/protein_id="NP_014389.3"
/db_xref="GeneID:855723"
/db_xref="SGD:S000004954"
/translation="MSKIKVVHPIVEMDGDEQTRVIWKLIKEKLILPYLDVDLKYYDL
SIQERDRTNDQVTKDSSYATLKYGVAVKCATITPDEARMKEFNLKEMWKSPNGTIRNI
LGGTVFREPIIIPKIPRLVPHWEKPIIIGRHAFGDQYRATDIKIKKAGKLRLQFSSDD
GKENIDLKVYEFPKSGGIAMAMFNTNDSIKGFAKASFELALKRKLPLFFTTKNTILKN
YDNQFKQIFDNLFDKEYKEKFQALKITYEHRLIDDMVAQMLKSKGGFIIAMKNYDGDV
QSDIVAQGFGSLGLMTSILITPDGKTFESEAAHGTVTRHFRKHQRGEETSTNSIASIF
AWTRAIIQRGKLDNTDDVIKFGNLLEKATLDTVQVGGKMTKDLALMLGKTNRSSYVTT
EEFIDEVAKRLQNMMLSSNEDKKGMCKL"
gene complement(<616211..>618241)
/gene="ASI3"
/locus_tag="YNL008C"
/db_xref="GeneID:855724"
mRNA complement(<616211..>618241)
/gene="ASI3"
/locus_tag="YNL008C"
/product="putative ubiquitin-protein ligase ASI3"
/transcript_id="NM_001182847.2"
/db_xref="GeneID:855724"
CDS complement(616211..618241)
/gene="ASI3"
/locus_tag="YNL008C"
/EC_number="2.3.2.27"
/experiment="EXISTENCE:direct assay:GO:0005637 nuclear
inner membrane [PMID:17085444]"
/experiment="EXISTENCE:direct assay:GO:0034399 nuclear
periphery [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0097658 Asi complex
[PMID:25236469]"
/experiment="EXISTENCE:genetic interaction:GO:0004842
ubiquitin-protein transferase activity [PMID:11454748]"
/experiment="EXISTENCE:genetic interaction:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:11454748]"
/experiment="EXISTENCE:mutant phenotype:GO:0006511
ubiquitin-dependent protein catabolic process
[PMID:25236469]"
/experiment="EXISTENCE:mutant phenotype:GO:0043161
proteasome-mediated ubiquitin-dependent protein catabolic
process [PMID:25253722]"
/experiment="EXISTENCE:mutant phenotype:GO:0071230
cellular response to amino acid stimulus [PMID:17085444]"
/note="Subunit of the inner nuclear membrane Asi ubiquitin
ligase complex; Asi complex targets both misfolded
proteins of the inner nuclear membrane-associated
degradation (INMAD) pathway and regulators of sterol
biosynthesis for ubiquitin-mediated degradation; acts with
Asi1p and Asi2p to ensure the fidelity of SPS-sensor
signaling by targeting latent unprocessed forms of Stp1p
and Stp2p, maintaining the repressed state of gene
expression in the absence of inducing amino acids"
/codon_start=1
/product="putative ubiquitin-protein ligase ASI3"
/protein_id="NP_014390.2"
/db_xref="GeneID:855724"
/db_xref="SGD:S000004953"
/translation="MSTNILQHVKQLLHNRDVFSFFHNKTGNLNYLDNTTQKPEVFVS
PNSTIVSAPTLDSFQALMEKGNFTTLQLAKVGIRMFFSYSVSKYAVLCFSTAIILNRL
TVMSSLRSNSTNIRLPLWSKTLLHLVATLSLVKALLQILSQFGLMHELHVSDTDFYAL
SVYLFVALSDCIEIFISSTTNVPSLICSDFSIWGLSLNLYIISKMPAGQQHIGDNVEL
LGAVFHRLVIHLVELFHIRAYRLCGEVILNAGFFTAFVTRTYLNGLDFINICLIHNYF
PGFFYISTILLASIGIFLKALFTSNPFRSLYSRYKNLEKWWRSNNYNGEEEFNEIALS
LCLLLTSNDYKIFKKSDNVKSVDEVAAFSNSYVVSGHLNQLQSTPEDLLSRKEMTTDS
QLPGFARTYLGLFELVRTIILTYSRLLKNLLWSKNFESSIDKKPRVGKRKKRDLNKYV
TEKNYKKFLYKPDVKELNIESDLRSLELLLPEDDSSKDYFPPRKIDESVSDEEFDSDM
ESQLIIDEEKELTHLSSNAVDSDDLEEIAWNISMWSILNYEMDVHNKVNGPLTRSQYG
KRNPQGVLVDVVIERLLHHTNSRYMYKRLNMKDDDKLEFKFDFAFDSCDEVEEMDLSC
LICKVNKRNIVTWPCRCLALCDDCRISLGYKGFATCVSCDSEVKGYSKLNIV"
rep_origin 616311..617125
/note="ARS1425; Putative replication origin; identified in
multiple array studies, not yet confirmed by plasmid-based
assay"
/db_xref="SGD:S000130144"
gene complement(<618507..>619565)
/gene="SIS1"
/locus_tag="YNL007C"
/db_xref="GeneID:855725"
mRNA complement(<618507..>619565)
/gene="SIS1"
/locus_tag="YNL007C"
/product="type II HSP40 co-chaperone SIS1"
/transcript_id="NM_001182846.1"
/db_xref="GeneID:855725"
CDS complement(618507..619565)
/gene="SIS1"
/locus_tag="YNL007C"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22718905]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:33326013]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:33293525]"
/experiment="EXISTENCE:direct assay:GO:0006457 protein
folding [PMID:9774392]"
/experiment="EXISTENCE:direct assay:GO:0022627 cytosolic
small ribosomal subunit [PMID:8513501]"
/experiment="EXISTENCE:direct assay:GO:0051787 misfolded
protein binding [PMID:33293525|PMID:23791384]"
/experiment="EXISTENCE:mutant phenotype:GO:0002236
detection of misfolded protein [PMID:33293525]"
/experiment="EXISTENCE:mutant phenotype:GO:0006413
translational initiation [PMID:8513501]"
/experiment="EXISTENCE:mutant phenotype:GO:0006457 protein
folding [PMID:14657253]"
/experiment="EXISTENCE:mutant phenotype:GO:0009267
cellular response to starvation [PMID:25853343]"
/experiment="EXISTENCE:mutant phenotype:GO:0034605
cellular response to heat [PMID:33293525]"
/experiment="EXISTENCE:mutant phenotype:GO:0035719 tRNA
import into nucleus [PMID:25853343]"
/experiment="EXISTENCE:mutant phenotype:GO:0070843
misfolded protein transport [PMID:23791384]"
/experiment="EXISTENCE:mutant phenotype:GO:0071630 nuclear
protein quality control by the ubiquitin-proteasome system
[PMID:23791384]"
/experiment="EXISTENCE:mutant phenotype:GO:0072671
mitochondria-associated ubiquitin-dependent protein
catabolic process [PMID:32118579]"
/note="Type II HSP40 co-chaperone that interacts with the
HSP70 protein Ssa1p; shuttles between cytosol and nucleus;
mediates delivery of misfolded proteins into the nucleus
for degradation; involved in proteasomal degradation of
misfolded cytosolic proteins; protein abundance increases
in response to DNA replication stress; polyQ aggregates
sequester Sis1p and interfere with clearance of misfolded
proteins; similar to bacterial DnaJ proteins and mammalian
DnaJB1"
/codon_start=1
/product="type II HSP40 co-chaperone SIS1"
/protein_id="NP_014391.1"
/db_xref="GeneID:855725"
/db_xref="SGD:S000004952"
/translation="MVKETKLYDLLGVSPSANEQELKKGYRKAALKYHPDKPTGDTEK
FKEISEAFEILNDPQKREIYDQYGLEAARSGGPSFGPGGPGGAGGAGGFPGGAGGFSG
GHAFSNEDAFNIFSQFFGGSSPFGGADDSGFSFSSYPSGGGAGMGGMPGGMGGMHGGM
GGMPGGFRSASSSPTYPEEETVQVNLPVSLEDLFVGKKKSFKIGRKGPHGASEKTQID
IQLKPGWKAGTKITYKNQGDYNPQTGRRKTLQFVIQEKSHPNFKRDGDDLIYTLPLSF
KESLLGFSKTIQTIDGRTLPLSRVQPVQPSQTSTYPGQGMPTPKNPSQRGNLIVKYKV
DYPISLNDAQKRAIDENF"
gene <620067..>620978
/gene="LST8"
/locus_tag="YNL006W"
/db_xref="GeneID:855726"
mRNA <620067..>620978
/gene="LST8"
/locus_tag="YNL006W"
/product="TOR complex subunit LST8"
/transcript_id="NM_001182845.3"
/db_xref="GeneID:855726"
CDS 620067..620978
/gene="LST8"
/locus_tag="YNL006W"
/experiment="EXISTENCE:direct assay:GO:0000139 Golgi
membrane [PMID:12719473]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0010008 endosome
membrane [PMID:12719473]"
/experiment="EXISTENCE:direct assay:GO:0034399 nuclear
periphery [PMID:25817432]"
/experiment="EXISTENCE:mutant phenotype:GO:0007165 signal
transduction [PMID:12719473]"
/experiment="EXISTENCE:mutant phenotype:GO:0030950
establishment or maintenance of actin cytoskeleton
polarity [PMID:12408816]"
/experiment="EXISTENCE:mutant phenotype:GO:0031505
fungal-type cell wall organization [PMID:12719473]"
/experiment="EXISTENCE:mutant phenotype:GO:0031929 TOR
signaling [PMID:12719473]"
/experiment="EXISTENCE:mutant phenotype:GO:0031930
mitochondria-nucleus signaling pathway [PMID:11742997]"
/experiment="EXISTENCE:mutant phenotype:GO:0043539 protein
serine/threonine kinase activator activity
[PMID:16002396]"
/experiment="EXISTENCE:physical interaction:GO:0001558
regulation of cell growth [PMID:12408816]"
/experiment="EXISTENCE:physical interaction:GO:0031931
TORC1 complex [PMID:12408816]"
/experiment="EXISTENCE:physical interaction:GO:0031932
TORC2 complex [PMID:12408816]"
/note="Subunit of TORC1 and TORC2 involved in TOR
signaling; TORC1 regulates nutrient responsive growth and
TORC2 regulates plasma membrane (PM) homeostasis and
cell-cycle dependent actin cytoskeleton polarization;
negative regulator of Rtg1p/3p and Gln3p transcription
factors and amino acid biosynthesis; binds to both Tor1p
and Tor2p, regulating the integrity and kinase activity of
TORC2; PM retention is dependent on Avo3p; WD-repeat
protein"
/codon_start=1
/product="TOR complex subunit LST8"
/protein_id="NP_014392.3"
/db_xref="GeneID:855726"
/db_xref="SGD:S000004951"
/translation="MSVILVSAGYDHTIRFWEALTGVCSRTIQHSDSQVNRLEITNDK
KLLATAGHQNVRLYDIRTTNPNPVASFEGHRGNVTSVSFQQDNRWMVTSSEDGTIKVW
DVRSPSIPRNYKHNAPVNEVVIHPNQGELISCDRDGNIRIWDLGENQCTHQLTPEDDT
SLQSLSMASDGSMLAAANTKGNCYVWEMPNHTDASHLKPVTKFRAHSTYITRILLSSD
VKHLATCSADHTARVWSIDDDFKLETTLDGHQRWVWDCAFSADSAYLVTASSDHYVRL
WDLSTREIVRQYGGHHKGAVCVALNDV"
gene complement(<621313..>622428)
/gene="MRP7"
/locus_tag="YNL005C"
/gene_synonym="bL27m"
/db_xref="GeneID:855727"
mRNA complement(<621313..>622428)
/gene="MRP7"
/locus_tag="YNL005C"
/gene_synonym="bL27m"
/product="mitochondrial 54S ribosomal protein bL27m MRP7"
/transcript_id="NM_001182844.1"
/db_xref="GeneID:855727"
CDS complement(621313..622428)
/gene="MRP7"
/locus_tag="YNL005C"
/gene_synonym="bL27m"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:12392552]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005762
mitochondrial large ribosomal subunit [PMID:12392552]"
/experiment="EXISTENCE:mutant phenotype:GO:0033617
mitochondrial respiratory chain complex IV assembly
[PMID:28931599]"
/note="Mitochondrial ribosomal protein of the large
subunit; involved together with Mrpl35p in assembly of
cytochrome c oxidase"
/codon_start=1
/product="mitochondrial 54S ribosomal protein bL27m MRP7"
/protein_id="NP_014393.1"
/db_xref="GeneID:855727"
/db_xref="SGD:S000004950"
/translation="MWNPILLDTSSFSFQKHVSGVFLQVRNATKRAAGSRTSMKDSAG
RRLGPKKYEGQDVSTGEIIMRQRGTKFYPGENVGIGKDHSIFALEPGVVRYYLDPFHP
KRKFIGVALRRDLKLPSPHFEPTVRRFGRFELTNKRAAYKEENSISRKDYLAKPNILK
QLEVRESKRKELQDKLSKVLRDELKLDIKDIELATSYLIRVRASLKNGYPIEDARFNS
RYYLKEEERLKARRESWTNEKLSESLSKIDECSDLLNSSTSFNNKLELHQYISEQEKQ
ALKAKLLEDLEKSQHLETKKDKNYIKALFKDACNFLTLSEEVHLRRKYLKSVFPETDS
TVETKSGKKSIVSRRFDYTKNKVEVIARSRRAFLSKL"
gene <622915..>624621
/gene="HRB1"
/locus_tag="YNL004W"
/gene_synonym="TOM34"
/db_xref="GeneID:855728"
mRNA join(<622915..622944,623287..>624621)
/gene="HRB1"
/locus_tag="YNL004W"
/gene_synonym="TOM34"
/product="mRNA-binding protein"
/transcript_id="NM_001182843.1"
/db_xref="GeneID:855728"
CDS join(622915..622944,623287..624621)
/gene="HRB1"
/locus_tag="YNL004W"
/gene_synonym="TOM34"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:14769921]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:14676199]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640|PMID:14769921]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9499403]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:9499403]"
/experiment="EXISTENCE:mutant phenotype:GO:0071028 nuclear
mRNA surveillance [PMID:24452287]"
/note="Poly(A+) RNA-binding guard protein; key
surveillance factor for the selective export of spliced
mRNAs from the nucleus to the cytoplasm; preference for
intron-containing genes; similar to Npl3p; HRB1 has a
paralog, GBP2, that arose from the whole genome
duplication"
/codon_start=1
/product="mRNA-binding protein"
/protein_id="NP_014394.2"
/db_xref="GeneID:855728"
/db_xref="SGD:S000004949"
/translation="MSDQERGSENNNRSRSRSRSPVRRRMSDDHGYERDNHLSRRSGN
YNGRRKFADTYRGSRDRGEYRGGRERSDYRERERFNNRDNPRSRDRYDDRRRGRDVTG
RYGNRRDDYPRSFRSRHNTRDDSRRGGFGSSGARGDYGPLLARELDSTYEEKVNRNYS
NSIFVGNLTYDSTPEDLTEFFSQIGKVVRADIITSRGHHRGMGTVEFTNSDDVDRAIR
QYDGAFFMDRKIFVRQDNPPPSNNIKERKALDRGELRHNRKTHEVIVKNLPASVNWQA
LKDIFKECGNVAHADVELDGDGVSTGSGTVSFYDIKDLHRAIEKYNGYSIEGNVLDVK
SKESVHNHSDGDDVDIPMDDSPVNEEARKFTENVVGGGERNRLIYCSNLPFSTAKSDL
YDLFETIGKVNNAELRYDSKGAPTGIAVVEYDNVDDADVCIERLNNYNYGGCDLDISY
AKRL"
gene complement(<624975..>625829)
/gene="PET8"
/locus_tag="YNL003C"
/gene_synonym="SAM5"
/db_xref="GeneID:855729"
mRNA complement(<624975..>625829)
/gene="PET8"
/locus_tag="YNL003C"
/gene_synonym="SAM5"
/product="Pet8p"
/transcript_id="NM_001182842.3"
/db_xref="GeneID:855729"
CDS complement(624975..625829)
/gene="PET8"
/locus_tag="YNL003C"
/gene_synonym="SAM5"
/experiment="EXISTENCE:direct assay:GO:0000095
S-adenosyl-L-methionine transmembrane transporter activity
[PMID:14609944]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:16823961|PMID:14576278|PMID:24769239|PMID:14609944]"
/experiment="EXISTENCE:direct assay:GO:0015805
S-adenosyl-L-methionine transport [PMID:14609944]"
/experiment="EXISTENCE:genetic interaction:GO:0000095
S-adenosyl-L-methionine transmembrane transporter activity
[PMID:14609944]"
/experiment="EXISTENCE:genetic interaction:GO:0015805
S-adenosyl-L-methionine transport [PMID:14609944]"
/experiment="EXISTENCE:mutant phenotype:GO:0000095
S-adenosyl-L-methionine transmembrane transporter activity
[PMID:14609944]"
/experiment="EXISTENCE:mutant phenotype:GO:0015805
S-adenosyl-L-methionine transport [PMID:14609944]"
/note="S-adenosylmethionine transporter of the
mitochondrial inner membrane; member of the mitochondrial
carrier family; required for biotin biosynthesis and
respiratory growth"
/codon_start=1
/product="Pet8p"
/protein_id="NP_014395.3"
/db_xref="GeneID:855729"
/db_xref="SGD:S000004948"
/translation="MNTFFLSLLSGAAAGTSTDLVFFPIDTIKTRLQAKGGFFANGGY
KGIYRGLGSAVVASAPGASLFFISYDYMKVKSRPYISKLYSQGSEQLIDTTTHMLSSS
IGEICACLVRVPAEVVKQRTQVHSTNSSWQTLQSILRNDNKEGLRKNLYRGWSTTIMR
EIPFTCIQFPLYEYLKKTWAKANGQSQVEPWKGAICGSIAGGIAAATTTPLDFLKTRL
MLNKTTASLGSVIIRIYREEGPAVFFSGVGPRTMWISAGGAIFLGMYETVHSLLSKSF
PTAGEMRA"
gene complement(<626174..>627142)
/gene="RLP7"
/locus_tag="YNL002C"
/gene_synonym="RPL7"
/db_xref="GeneID:855730"
mRNA complement(<626174..>627142)
/gene="RLP7"
/locus_tag="YNL002C"
/gene_synonym="RPL7"
/product="Rlp7p"
/transcript_id="NM_001182841.3"
/db_xref="GeneID:855730"
CDS complement(626174..627142)
/gene="RLP7"
/locus_tag="YNL002C"
/gene_synonym="RPL7"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:11087857]"
/experiment="EXISTENCE:direct assay:GO:0030687
preribosome, large subunit precursor
[PMID:11583614|PMID:17443350]"
/experiment="EXISTENCE:direct assay:GO:0042134 rRNA
primary transcript binding [PMID:24129494]"
/experiment="EXISTENCE:direct assay:GO:0042273 ribosomal
large subunit biogenesis [PMID:23945946]"
/experiment="EXISTENCE:mutant phenotype:GO:0000463
maturation of LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11087857]"
/experiment="EXISTENCE:mutant phenotype:GO:0000465
exonucleolytic trimming to generate mature 5'-end of 5.8S
rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S
rRNA, LSU-rRNA) [PMID:11087857]"
/experiment="EXISTENCE:mutant phenotype:GO:0042273
ribosomal large subunit biogenesis [PMID:11087857]"
/note="Nucleolar protein similar to large ribosomal
subunit L7 proteins; constituent of 66S pre-ribosomal
particles; plays an essential role in processing of
precursors to the large ribosomal subunit RNAs; binds
junction of ITS2 and ITS2-proximal stem between the 3' end
of 5.8S rRNA and the 5' end of 25S rRNA"
/codon_start=1
/product="Rlp7p"
/protein_id="NP_014396.3"
/db_xref="GeneID:855730"
/db_xref="SGD:S000004947"
/translation="MSSTQDSKAQTLNSNPEILLRKRRNADRTRIERQELAKKKREEQ
IKKKRSNKNKFVRAESIVAKTLATSREKERIKRVSILEDKKAKNETQHIASGKDFILK
ITEKANGAEENSVDLEETEEEEDDGLIREKTTYDGKPALLFIVRVRGPLAVNIPNKAF
KILSLLRLVETNTGVFVKLTKNVYPLLKVIAPYVVIGKPSLSSIRSLIQKRGRIIYKG
ENEAEPHEIVLNDNNIVEEQLGDHGIICVEDIIHEIATMGESFSVCNFFLQPFKLNRE
VSGFGSLNRLRKIKQREAESRTRQFSNAATAPVIEVDIDSLLAKLN"
gene <627456..>628616
/gene="DOM34"
/locus_tag="YNL001W"
/db_xref="GeneID:855731"
mRNA <627456..>628616
/gene="DOM34"
/locus_tag="YNL001W"
/product="ribosome dissociation factor DOM34"
/transcript_id="NM_001182840.1"
/db_xref="GeneID:855731"
CDS 627456..628616
/gene="DOM34"
/locus_tag="YNL001W"
/experiment="EXISTENCE:direct assay:GO:0004521 RNA
endonuclease activity [PMID:17889667]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0032790 ribosome
disassembly [PMID:20947765|PMID:24424461]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:21623367]"
/experiment="EXISTENCE:direct assay:GO:1990533 Dom34-Hbs1
complex [PMID:21623367]"
/experiment="EXISTENCE:genetic interaction:GO:0070966
nuclear-transcribed mRNA catabolic process, no-go decay
[PMID:16554824]"
/experiment="EXISTENCE:mutant phenotype:GO:0032790
ribosome disassembly [PMID:24424461|PMID:22770215]"
/experiment="EXISTENCE:mutant phenotype:GO:0045727
positive regulation of translation [PMID:24424461]"
/experiment="EXISTENCE:mutant phenotype:GO:0070651
nonfunctional rRNA decay [PMID:19481524]"
/experiment="EXISTENCE:mutant phenotype:GO:0070966
nuclear-transcribed mRNA catabolic process, no-go decay
[PMID:19420139]"
/note="Protein that facilitates ribosomal subunit
dissociation; Dom34-Hbs1 complex and Rli1p have roles in
dissociating inactive ribosomes to facilitate translation
restart, particularly ribosomes stalled in 3' UTRs;
required for RNA cleavage in no-go decay, but reports
conflict on endonuclease activity; Pelota ortholog;
protein abundance increases in response to DNA replication
stress; DOM34 has a paralog, YCL001W-B, that arose from
the whole genome duplication"
/codon_start=1
/product="ribosome dissociation factor DOM34"
/protein_id="NP_014397.1"
/db_xref="GeneID:855731"
/db_xref="SGD:S000004946"
/translation="MKVISLKKDSFNKGGAVITLLPEDKEDLFTVYQIVDKDDELIFK
KKFTSKLDEAGKKKSTDLVKLKIKVISEDFDMKDEYLKYKGVTVTDESGASNVDIPVG
KYLSFTLDYVYPFTIIKQNFNKFMQKLLNEACNIEYKSDTAAVVLQEGIAHVCLVTSS
STILKQKIEYSMPKKKRTTDVLKFDEKTEKFYKAIYSAMKKDLNFDKLKTIILCSPGF
YAKILMDKIFQYAEEEHNKKILDNKGMFFIAHCSTGYLQGINEVLKNPLYASKLQDTK
YSKEIMVMDEFLLHLNKDDDKAWYGEKEVVKAAEYGAISYLLLTDKVLHSDNIAQREE
YLKLMDSVESNGGKALVLSTLHSLGEELDQLTGIACILKYPLPDLDEDDGEE"
centromere 628758..628875
/note="CEN14; Chromosome XIV centromere"
/db_xref="SGD:S000006475"
centromere 628758..628767
/note="CEN14_CDEI of CEN14"
centromere 628768..628850
/note="CEN14_CDEII of CEN14"
centromere 628851..628875
/note="CEN14_CDEIII of CEN14"
gene complement(<629622..>631061)
/gene="CIT1"
/locus_tag="YNR001C"
/gene_synonym="LYS6"
/db_xref="GeneID:855732"
mRNA complement(<629622..>631061)
/gene="CIT1"
/locus_tag="YNR001C"
/gene_synonym="LYS6"
/product="citrate (Si)-synthase CIT1"
/transcript_id="NM_001183178.1"
/db_xref="GeneID:855732"
CDS complement(629622..631061)
/gene="CIT1"
/locus_tag="YNR001C"
/gene_synonym="LYS6"
/EC_number="2.3.3.1"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:24769239|PMID:11914276|PMID:14576278|PMID:16823961|P
MID:11502169]"
/experiment="EXISTENCE:direct assay:GO:0036440 citrate
synthase activity [PMID:17570335]"
/experiment="EXISTENCE:direct assay:GO:0046356 acetyl-CoA
catabolic process [PMID:17570335]"
/experiment="EXISTENCE:genetic interaction:GO:0006101
citrate metabolic process [PMID:12913175]"
/experiment="EXISTENCE:mutant phenotype:GO:0036440 citrate
synthase activity [PMID:17570335]"
/experiment="EXISTENCE:mutant phenotype:GO:0046356
acetyl-CoA catabolic process [PMID:17570335]"
/experiment="EXISTENCE:physical interaction:GO:0005739
mitochondrion [PMID:16962558]"
/note="Mitochondrial citrate synthase; catalyzes
condensation of acetyl coenzyme A and oxaloacetate to form
citrate, which is the first and rate-limiting step of the
TCA cycle; transcription subject to glucose repression;
CIT1 has a paralog, CIT2, that arose from the whole genome
duplication"
/codon_start=1
/product="citrate (Si)-synthase CIT1"
/protein_id="NP_014398.1"
/db_xref="GeneID:855732"
/db_xref="SGD:S000005284"
/translation="MSAILSTTSKSFLSRGSTRQCQNMQKALFALLNARHYSSASEQT
LKERFAEIIPAKAEEIKKFKKEHGKTVIGEVLLEQAYGGMRGIKGLVWEGSVLDPEEG
IRFRGRTIPEIQRELPKAEGSTEPLPEALFWLLLTGEIPTDAQVKALSADLAARSEIP
EHVIQLLDSLPKDLHPMAQFSIAVTALESESKFAKAYAQGVSKKEYWSYTFEDSLDLL
GKLPVIASKIYRNVFKDGKITSTDPNADYGKNLAQLLGYENKDFIDLMRLYLTIHSDH
EGGNVSAHTTHLVGSALSSPYLSLAAGLNGLAGPLHGRANQEVLEWLFKLREEVKGDY
SKETIEKYLWDTLNAGRVVPGYGHAVLRKTDPRYTAQREFALKHFPDYELFKLVSTIY
EVAPGVLTKHGKTKNPWPNVDSHSGVLLQYYGLTEASFYTVLFGVARAIGVLPQLIID
RAVGAPIERPKSFSTEKYKELVKKIESKN"
gene complement(631846..631917)
/gene="SUF10"
/locus_tag="YNCN0016C"
/db_xref="GeneID:855734"
tRNA complement(631846..631917)
/gene="SUF10"
/locus_tag="YNCN0016C"
/product="tRNA-Pro"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE
analysis; can mutate to suppress +1 frameshift mutations
in proline codons"
/db_xref="GeneID:855734"
/db_xref="SGD:S000006679"
repeat_region complement(631998..632114)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007143"
repeat_region complement(632119..632489)
/note="Ty4 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007146"
gene 632599..632672
/locus_tag="YNCN0017W"
/db_xref="GeneID:855735"
tRNA 632599..632672
/locus_tag="YNCN0017W"
/product="tRNA-Asn"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Asparagine tRNA (tRNA-Asn), predicted by
tRNAscan-SE analysis"
/db_xref="GeneID:855735"
/db_xref="SGD:S000006674"
gene complement(<633008..>633856)
/gene="ATO2"
/locus_tag="YNR002C"
/gene_synonym="FUN34"
/db_xref="GeneID:855736"
mRNA complement(<633008..>633856)
/gene="ATO2"
/locus_tag="YNR002C"
/gene_synonym="FUN34"
/product="putative ammonium permease ATO2"
/transcript_id="NM_001183179.3"
/db_xref="GeneID:855736"
CDS complement(633008..633856)
/gene="ATO2"
/locus_tag="YNR002C"
/gene_synonym="FUN34"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:17395151]"
/experiment="EXISTENCE:mutant phenotype:GO:0008519
ammonium channel activity [PMID:12429834]"
/experiment="EXISTENCE:mutant phenotype:GO:0019740
nitrogen utilization [PMID:12429834]"
/experiment="EXISTENCE:mutant phenotype:GO:0072488
ammonium transmembrane transport [PMID:12429834]"
/note="Putative transmembrane protein involved in export
of ammonia; ammonia is a starvation signal that promotes
cell death in aging colonies; phosphorylated in
mitochondria; member of the TC 9.B.33 YaaH family; homolog
of Y. lipolytica Gpr1p; ATO2 has a paralog, ADY2, that
arose from the whole genome duplication"
/codon_start=1
/product="putative ammonium permease ATO2"
/protein_id="NP_014399.3"
/db_xref="GeneID:855736"
/db_xref="SGD:S000005285"
/translation="MSDREQSSGNTAFENPKALDSSEGEFISENNDQSRHSQESICKI
YTAGKNNEYIYIGRQKFLRDDLFEAFGGTLNPGLAPAPVHKFANPAPLGLSGFALTTF
VLSMFNARAQGITIPNVVVGCAMFYGGLVQLIAGIWEIALENTFGGTALCSFGGFWLS
FGAIYIPWFGILDAYKDKESDLGNALGFYLLGWALFTFGLSVCTMKSTIMFFALFFLL
AVTFLLLSIANFTGEVGVTRAGGVLGVIVAFIAWYNAYAGIATRQNSYIMVHPFALPS
NDKVFF"
gene complement(<634344..>635297)
/gene="RPC34"
/locus_tag="YNR003C"
/db_xref="GeneID:855737"
mRNA complement(<634344..>635297)
/gene="RPC34"
/locus_tag="YNR003C"
/product="DNA-directed RNA polymerase III subunit C34"
/transcript_id="NM_001183180.1"
/db_xref="GeneID:855737"
CDS complement(634344..635297)
/gene="RPC34"
/locus_tag="YNR003C"
/experiment="EXISTENCE:direct assay:GO:0003899
DNA-directed RNA polymerase activity [PMID:3905793]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005666 RNA
polymerase III complex [PMID:3905793|PMID:10611227]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0042797 tRNA
transcription by RNA polymerase III [PMID:3905793]"
/experiment="EXISTENCE:mutant phenotype:GO:0042797 tRNA
transcription by RNA polymerase III [PMID:1400451]"
/experiment="EXISTENCE:physical interaction:GO:0005739
mitochondrion [PMID:16962558]"
/note="RNA polymerase III subunit C34; interacts with
TFIIIB70 and is a key determinant in pol III recruitment
by the preinitiation complex"
/codon_start=1
/product="DNA-directed RNA polymerase III subunit C34"
/protein_id="NP_014400.1"
/db_xref="GeneID:855737"
/db_xref="SGD:S000005286"
/translation="MSGMIENGLQLSDNAKTLHSQMMSKGIGALFTQQELQKQMGIGS
LTDLMSIVQELLDKNLIKLVKQNDELKFQGVLESEAQKKATMSAEEALVYSYIEASGR
EGIWSKTIKARTNLHQHVVLKCLKSLESQRYVKSVKSVKFPTRKIYMLYSLQPSVDIT
GGPWFTDGELDIEFINSLLTIVWRFISENTFPNGFKNFENGPKKNVFYAPNVKNYSTT
QEILEFITAAQVANVELTPSNIRSLCEVLVYDDKLEKVTHDCYRVTLESILQMNQGEG
EPEAGNKALEDEEEFSIFNYFKMFPASKHDKEVVYFDEWTI"
rep_origin 635664..635904
/note="ARS1426; Autonomously Replicating Sequence"
/db_xref="SGD:S000118392"
gene <635943..>636383
/gene="SWM2"
/locus_tag="YNR004W"
/db_xref="GeneID:855738"
mRNA <635943..>636383
/gene="SWM2"
/locus_tag="YNR004W"
/product="Swm2p"
/transcript_id="NM_001183181.1"
/db_xref="GeneID:855738"
CDS 635943..636383
/gene="SWM2"
/locus_tag="YNR004W"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0008033 tRNA
processing [PMID:27348091]"
/experiment="EXISTENCE:mutant phenotype:GO:0036261
7-methylguanosine cap hypermethylation [PMID:20621096]"
/note="Protein with a role in snRNA and snoRNA cap
trimethylation; interacts with Tgs1p and shows similar
phenotypes; required for trimethylation of the caps of
spliceosomal snRNAs and the U3 snoRNA, and for efficient
3' end processing of U3 snoRNA; may act as a specificity
factor for Tgs1p"
/codon_start=1
/product="Swm2p"
/protein_id="NP_014401.1"
/db_xref="GeneID:855738"
/db_xref="SGD:S000005287"
/translation="MIDLYNYSNLEGLLDGLTDLNRIPKEYSAVLEPYFQNIARNAHL
KSRALKICRSNFHKWNEEGAKTVNPEIIRRCLNLWYVLKGKEYKKLKDPPPADNIIKD
EIDVSYVKNLNVVRLEFDEFGKLISNPLENLILEEVEVNDFIQE"
gene <636986..>638854
/gene="VPS27"
/locus_tag="YNR006W"
/gene_synonym="DID7; GRD11; SSV17; VPL23; VPT27"
/db_xref="GeneID:855739"
mRNA <636986..>638854
/gene="VPS27"
/locus_tag="YNR006W"
/gene_synonym="DID7; GRD11; SSV17; VPL23; VPT27"
/product="ESCRT-0 subunit protein VPS27"
/transcript_id="NM_001183183.3"
/db_xref="GeneID:855739"
CDS 636986..638854
/gene="VPS27"
/locus_tag="YNR006W"
/gene_synonym="DID7; GRD11; SSV17; VPL23; VPT27"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:12055639]"
/experiment="EXISTENCE:direct assay:GO:0005774 vacuolar
membrane [PMID:31740006]"
/experiment="EXISTENCE:direct assay:GO:0032266
phosphatidylinositol-3-phosphate binding [PMID:9702203]"
/experiment="EXISTENCE:direct assay:GO:0036435 K48-linked
polyubiquitin modification-dependent protein binding
[PMID:28525741]"
/experiment="EXISTENCE:direct assay:GO:0043130 ubiquitin
binding [PMID:20150893|PMID:12055639]"
/experiment="EXISTENCE:direct assay:GO:0070530 K63-linked
polyubiquitin modification-dependent protein binding
[PMID:28525741]"
/experiment="EXISTENCE:mutant phenotype:GO:0006623 protein
targeting to vacuole [PMID:12055639]"
/experiment="EXISTENCE:mutant phenotype:GO:0006995
cellular response to nitrogen starvation [PMID:32726584]"
/experiment="EXISTENCE:mutant phenotype:GO:0016237
microautophagy [PMID:31740006]"
/experiment="EXISTENCE:mutant phenotype:GO:0045053 protein
retention in Golgi apparatus [PMID:8649377]"
/experiment="EXISTENCE:mutant phenotype:GO:0045324 late
endosome to vacuole transport [PMID:12055639]"
/experiment="EXISTENCE:mutant phenotype:GO:0140504
microlipophagy [PMID:32726584]"
/experiment="EXISTENCE:mutant phenotype:GO:1904669 ATP
export [PMID:26585826]"
/experiment="EXISTENCE:physical interaction:GO:0006623
protein targeting to vacuole [PMID:12055639]"
/experiment="EXISTENCE:physical interaction:GO:0033565
ESCRT-0 complex [PMID:12055639]"
/note="Ubiquitin binding protein involved in endosomal
protein sorting; K48-, K63-Ub chain binding protein that
preferentially binds K63-linked chains in vitro; subunit
of the ESCRT-0 complex that binds to ubiquitin via two
ubiquitin-interacting motifs (UIMs) and is involved in
sorting cargo into multivesicular bodies; required for
recycling Golgi proteins, forming lumenal membranes and
sorting ubiquitinated proteins destined for degradation"
/codon_start=1
/product="ESCRT-0 subunit protein VPS27"
/protein_id="NP_014403.3"
/db_xref="GeneID:855739"
/db_xref="SGD:S000005289"
/translation="MSVSTPSELDALIEQATSESIPNGDLDLPIALEISDVLRSRRVN
PKDSMRCIKKRILNTADNPNTQLSSWKLTNICVKNGGTPFIKEICSREFMDTMEHVIL
REDSNEELSELVKTILYELYVAFKNDSQLNYVAKVYDKLISRGIKFPEKLTLSNSPTA
MFDSKTPADWIDSDACMICSKKFSLLNRKHHCRSCGGVFCQEHSSNSIPLPDLGIYEP
VRVCDSCFEDYDLKRHDDSKKSKKHRHKRKKDRDYSTPEDEEELIRKAIELSLKESRN
SASSEPIVPVVESKNEVKRQEIEEEEDPDLKAAIQESLREAEEAKLRSERQKASRQMQ
PQQPSPQPQPIHSVDLSDEEKDSIYMFASLVEKMKSRPLNEILEDSKLQNLAQRVFAS
KARLNYALNDKAQKYNTLIEMNGKISEIMNIYDRLLEQQLQSINLSQQYTLPQVPSDP
YNYLTENVQNPAESYQTPPLQQLSSHQYKPQQDVSRQQSVKANSSPTTNIDHLKTIDV
TPHAQQKPQSHVELAPSDPPYPKEEAEDEGTQAVQDEESSTQESRERPYPVETENGET
SINKRPQGITRYDFPTVPARKFVQPESTVPLPASSSEIPIKEERPPSPQEELLIEL"
gene complement(<639182..>640114)
/gene="ATG3"
/locus_tag="YNR007C"
/gene_synonym="APG3; AUT1"
/db_xref="GeneID:855741"
mRNA complement(<639182..>640114)
/gene="ATG3"
/locus_tag="YNR007C"
/gene_synonym="APG3; AUT1"
/product="Atg3p"
/transcript_id="NM_001183184.3"
/db_xref="GeneID:855741"
CDS complement(639182..640114)
/gene="ATG3"
/locus_tag="YNR007C"
/gene_synonym="APG3; AUT1"
/experiment="EXISTENCE:direct assay:GO:0000407 phagophore
assembly site [PMID:25680528|PMID:25645919]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0006501 C-terminal
protein lipidation [PMID:15277523]"
/experiment="EXISTENCE:direct assay:GO:0061908 phagophore
[PMID:25645919|PMID:25680528]"
/experiment="EXISTENCE:mutant phenotype:GO:0000045
autophagosome assembly [PMID:25680528]"
/experiment="EXISTENCE:mutant phenotype:GO:0000422
autophagy of mitochondrion [PMID:19793921]"
/experiment="EXISTENCE:mutant phenotype:GO:0006501
C-terminal protein lipidation
[PMID:11100732|PMID:15277523]"
/experiment="EXISTENCE:mutant phenotype:GO:0019776
Atg8-family ligase activity [PMID:11100732]"
/experiment="EXISTENCE:mutant phenotype:GO:0032258
cytoplasm to vacuole targeting by the Cvt pathway
[PMID:9023185]"
/experiment="EXISTENCE:mutant phenotype:GO:0034727
piecemeal microautophagy of the nucleus [PMID:18701704]"
/experiment="EXISTENCE:mutant phenotype:GO:0044804
nucleophagy [PMID:22768199]"
/experiment="EXISTENCE:mutant phenotype:GO:0061908
phagophore [PMID:25680528]"
/note="E2-like enzyme; involved in autophagy and
cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role
in formation of Atg8p-phosphatidylethanolamine conjugates,
which are involved in membrane dynamics during autophagy
and Cvt; interaction with Atg8p regulated by acetylation
by NuA4 histone acetyltransferase Esa1p while attenuation
of Atg3 acetylation is mediated by histone deacetylase
Rpd3p; Atg12p-Atg5p conjugate enhances E2 activity of
Atg3p by rearranging its catalytic site"
/codon_start=1
/product="Atg3p"
/protein_id="NP_014404.3"
/db_xref="GeneID:855741"
/db_xref="SGD:S000005290"
/translation="MIRSTLSSWREYLTPITHKSTFLTTGQITPEEFVQAGDYLCHMF
PTWKWNEESSDISYRDFLPKNKQFLIIRKVPCDKRAEQCVEVEGPDVIMKGFAEDGDE
DDVLEYIGSETEHVQSTPAGGTKDSSIDDIDELIQDMEIKEEDENDDTEEFNAKGGLA
KDMAQERYYDLYIAYSTSYRVPKMYIVGFNSNGSPLSPEQMFEDISADYRTKTATIEK
LPFYKNSVLSVSIHPCKHANVMKILLDKVRVVRQRRRKELQEEQELDGVGDWEDLQDD
IDDSLRVDQYLIVFLKFITSVTPSIQHDYTMEGW"
gene <640396..>642381
/gene="LRO1"
/locus_tag="YNR008W"
/db_xref="GeneID:855742"
mRNA <640396..>642381
/gene="LRO1"
/locus_tag="YNR008W"
/product="phospholipid:diacylglycerol acyltransferase"
/transcript_id="NM_001183185.1"
/db_xref="GeneID:855742"
CDS 640396..642381
/gene="LRO1"
/locus_tag="YNR008W"
/EC_number="2.3.1.158"
/experiment="EXISTENCE:direct assay:GO:0005637 nuclear
inner membrane [PMID:31422915]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005811 lipid
droplet [PMID:22454508]"
/experiment="EXISTENCE:direct assay:GO:0006672 ceramide
metabolic process [PMID:22738231]"
/experiment="EXISTENCE:direct assay:GO:0019432
triglyceride biosynthetic process [PMID:10747858]"
/experiment="EXISTENCE:direct assay:GO:0019915 lipid
storage [PMID:10747858]"
/experiment="EXISTENCE:direct assay:GO:0046027
phospholipid:diacylglycerol acyltransferase activity
[PMID:10747858]"
/experiment="EXISTENCE:direct assay:GO:0055091
phospholipid homeostasis [PMID:31422915]"
/experiment="EXISTENCE:direct assay:GO:0097038 perinuclear
endoplasmic reticulum [PMID:22454508]"
/experiment="EXISTENCE:mutant phenotype:GO:0006672
ceramide metabolic process [PMID:22738231]"
/experiment="EXISTENCE:mutant phenotype:GO:0019432
triglyceride biosynthetic process [PMID:10747858]"
/experiment="EXISTENCE:mutant phenotype:GO:0019915 lipid
storage [PMID:10747858]"
/note="Acyltransferase that converts diacylglycerol to
triacylglycerol (TGA); one of several acyltransferases
that contribute to TGA synthesis; relocates from ER to
nuclear membrane in response cell cycle and starvation
signals; Lro1p and Dga1p can O-acylate ceramides; putative
homolog of human lecithin cholesterol acyltransferase"
/codon_start=1
/product="phospholipid:diacylglycerol acyltransferase"
/protein_id="NP_014405.1"
/db_xref="GeneID:855742"
/db_xref="SGD:S000005291"
/translation="MGTLFRRNVQNQKSDSDENNKGGSVHNKRESRNHIHHQQGLGHK
RRRGISGSAKRNERGKDFDRKRDGNGRKRWRDSRRLIFILGAFLGVLLPFSFGAYHVH
NSDSDLFDNFVNFDSLKVYLDDWKDVLPQGISSFIDDIQAGNYSTSSLDDLSENFAVG
KQLLRDYNIEAKHPVVMVPGVISTGIESWGVIGDDECDSSAHFRKRLWGSFYMLRTMV
MDKVCWLKHVMLDPETGLDPPNFTLRAAQGFESTDYFIAGYWIWNKVFQNLGVIGYEP
NKMTSAAYDWRLAYLDLERRDRYFTKLKEQIELFHQLSGEKVCLIGHSMGSQIIFYFM
KWVEAEGPLYGNGGRGWVNEHIDSFINAAGTLLGAPKAVPALISGEMKDTIQLNTLAM
YGLEKFFSRIERVKMLQTWGGIPSMLPKGEEVIWGDMKSSSEDALNNNTDTYGNFIRF
ERNTSDAFNKNLTMKDAINMTLSISPEWLQRRVHEQYSFGYSKNEEELRKNELHHKHW
SNPMEVPLPEAPHMKIYCIYGVNNPTERAYVYKEEDDSSALNLTIDYESKQPVFLTEG
DGTVPLVAHSMCHKWAQGASPYNPAGINVTIVEMKHQPDRFDIRGGAKSAEHVDILGS
AELNDYILKIASGNGDLVEPRQLSNLSQWVSQMPFPM"
gene <642690..>643439
/gene="NRM1"
/locus_tag="YNR009W"
/db_xref="GeneID:855743"
mRNA <642690..>643439
/gene="NRM1"
/locus_tag="YNR009W"
/product="Nrm1p"
/transcript_id="NM_001183186.1"
/db_xref="GeneID:855743"
CDS 642690..643439
/gene="NRM1"
/locus_tag="YNR009W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0030907 MBF
transcription complex [PMID:16916637]"
/experiment="EXISTENCE:mutant phenotype:GO:0000082 G1/S
transition of mitotic cell cycle [PMID:16916637]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:16916637]"
/experiment="EXISTENCE:physical interaction:GO:0140297
DNA-binding transcription factor binding [PMID:16916637]"
/note="Transcriptional co-repressor of MBF-regulated gene
expression; Nrm1p associates stably with promoters via MCB
binding factor (MBF) to repress transcription upon exit
from G1 phase"
/codon_start=1
/product="Nrm1p"
/protein_id="NP_014406.1"
/db_xref="GeneID:855743"
/db_xref="SGD:S000005292"
/translation="MSIMKQRLPLGEFSSSKINKLAIANIADASEPRNHGENNVGTVC
LPSIKSLMVSPEVYENTKSLPVPLMRSSGGGMACASKSSCQDGISTKTTSRDYSELSK
KLQIRLQFAYYKYKTKQTDKNFTDLKSKHSITRPSKVATHSKSEPLTRRRKLVLSQGH
YKTPARSKIKTPSSICSHDNTSSFTSFRGVSESSSTTADMNVADTTTPIRNNINTKHS
NSHNRTLYQRQETPTSIKAAKSLIHLFTSNQ"
gene <643744..>644193
/gene="CSE2"
/locus_tag="YNR010W"
/gene_synonym="MED9"
/db_xref="GeneID:855744"
mRNA <643744..>644193
/gene="CSE2"
/locus_tag="YNR010W"
/gene_synonym="MED9"
/product="Cse2p"
/transcript_id="NM_001183187.3"
/db_xref="GeneID:855744"
CDS 643744..644193
/gene="CSE2"
/locus_tag="YNR010W"
/gene_synonym="MED9"
/experiment="EXISTENCE:direct assay:GO:0003713
transcription coactivator activity [PMID:19077037]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0051123 RNA
polymerase II preinitiation complex assembly
[PMID:19077037]"
/experiment="EXISTENCE:direct assay:GO:0070847 core
mediator complex [PMID:9812975]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:11470794]"
/experiment="EXISTENCE:mutant phenotype:GO:0005198
structural molecule activity [PMID:19077037]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:9891034]"
/note="Subunit of the RNA polymerase II mediator complex;
associates with core polymerase subunits to form the RNA
polymerase II holoenzyme; component of the Middle domain
of mediator; required for regulation of RNA polymerase II
activity; relocalizes to the cytosol in response to
hypoxia"
/codon_start=1
/product="Cse2p"
/protein_id="NP_014407.3"
/db_xref="GeneID:855744"
/db_xref="SGD:S000005293"
/translation="MNLQNNVLNQIHQILLPTNPTLDKPNAEATKEEFSSAENRDEKD
YLTNQQPKNLSTPSTSSNGEFIPHIFYSLHQIRKDPNNLSNQLETLTGSIRHRLKLCK
SLISENEDTKDLLSKSPSEWQDIIHQREQELQIKRDVLDDLYRKLQR"
gene complement(<644320..>646950)
/gene="PRP2"
/locus_tag="YNR011C"
/gene_synonym="RNA2"
/db_xref="GeneID:855745"
mRNA complement(<644320..>646950)
/gene="PRP2"
/locus_tag="YNR011C"
/gene_synonym="RNA2"
/product="DEAH-box RNA-dependent ATPase PRP2"
/transcript_id="NM_001183188.1"
/db_xref="GeneID:855745"
CDS complement(644320..646950)
/gene="PRP2"
/locus_tag="YNR011C"
/gene_synonym="RNA2"
/EC_number="3.6.4.13"
/experiment="EXISTENCE:direct assay:GO:0000349 generation
of catalytic spliceosome for first transesterification
step [PMID:8943336]"
/experiment="EXISTENCE:direct assay:GO:0008186
ATP-dependent activity, acting on RNA [PMID:1534753]"
/experiment="EXISTENCE:direct assay:GO:0071006 U2-type
catalytic step 1 spliceosome [PMID:2251118]"
/experiment="EXISTENCE:mutant phenotype:GO:0034247 snoRNA
splicing [PMID:24442613]"
/note="RNA-dependent DExH-box ATPase/helicase; required
for activation of spliceosome before first
transesterification step in RNA splicing; implicated in
rearranging and proofreading snRNA structure in catalytic
activation of spliceosome; ortholog of human protein
DHX16"
/codon_start=1
/product="DEAH-box RNA-dependent ATPase PRP2"
/protein_id="NP_014408.1"
/db_xref="GeneID:855745"
/db_xref="SGD:S000005294"
/translation="MSSITSETGKRRVKRTYEVTRQNDNAVRIEPSSLGEEEDKEAKD
KNSALQLKRSRYDPNKVFSNTNQGPEKNNLKGEQLGSQKKSSKYDEKITSNNELTTKK
GLLGDSENETKYASSNSKFNVEVTHKIKNAKEIDKINRQRMWEEQQLRNAMAGQSDHP
DDITLEGSDKYDYVFDTDAMIDYTNEEDDLLPEEKLQYEARLAQALETEEKRILTIQE
ARKLLPVHQYKDELLQEIKKNQVLIIMGETGSGKTTQLPQYLVEDGFTDQGKLQIAIT
QPRRVAATSVAARVADEMNVVLGKEVGYQIRFEDKTTPNKTVLKYMTDGMLLREFLTD
SKLSKYSCIMIDEAHERTLATDILIGLLKDILPQRPTLKLLISSATMNAKKFSEFFDN
CPIFNVPGRRYPVDIHYTLQPEANYIHAAITTIFQIHTTQSLPGDILVFLTGQEEIER
TKTKLEEIMSKLGSRTKQMIITPIYANLPQEQQLKIFQPTPENCRKVVLATNIAETSL
TIDGIRYVIDPGFVKENSYVPSTGMTQLLTVPCSRASVDQRAGRAGRVGPGKCFRIFT
KWSYLHELELMPKPEITRTNLSNTVLLLLSLGVTDLIKFPLMDKPSIPTLRKSLENLY
ILGALNSKGTITRLGKMMCEFPCEPEFAKVLYTAATHEQCQGVLEECLTIVSMLHETP
SLFIGQKRDAAASVLSEVESDHILYLEIFNQWRNSKFSRSWCQDHKIQFKTMLRVRNI
RNQLFRCSEKVGLVEKNDQARMKIGNIAGYINARITRCFISGFPMNIVQLGPTGYQTM
GRSSGGLNVSVHPTSILFVNHKEKAQRPSKYVLYQQLMLTSKEFIRDCLVIPKEEWLI
DMVPQIFKDLIDDKTNRGRR"
gene <647432..>648937
/gene="URK1"
/locus_tag="YNR012W"
/db_xref="GeneID:855746"
mRNA <647432..>648937
/gene="URK1"
/locus_tag="YNR012W"
/product="uridine kinase URK1"
/transcript_id="NM_001183189.1"
/db_xref="GeneID:855746"
CDS 647432..648937
/gene="URK1"
/locus_tag="YNR012W"
/EC_number="2.7.1.48"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0004849 uridine
kinase activity [PMID:10501935]"
/experiment="EXISTENCE:mutant phenotype:GO:0008655
pyrimidine-containing compound salvage [PMID:11872485]"
/note="Uridine/cytidine kinase; component of the
pyrimidine ribonucleotide salvage pathway that converts
uridine into UMP and cytidine into CMP; involved in the
pyrimidine deoxyribonucleotide salvage pathway, converting
deoxycytidine into dCMP"
/codon_start=1
/product="uridine kinase URK1"
/protein_id="NP_014409.1"
/db_xref="GeneID:855746"
/db_xref="SGD:S000005295"
/translation="MSHRIAPSKERSSSFISILDDETRDTLKANAVMDGEVDVKKTKG
KSSRYIPPWTTPYIIGIGGASGSGKTSVAAKIVSSINVPWTVLISLDNFYNPLGPEDR
ARAFKNEYDFDEPNAINLDLAYKCILNLKEGKRTNIPVYSFVHHNRVPDKNIVIYGAS
VVVIEGIYALYDRRLLDLMDLKIYVDADLDVCLARRLSRDIVSRGRDLDGCIQQWEKF
VKPNAVKFVKPTMKNADAIIPSMSDNATAVNLIINHIKSKLELKSNEHLRELIKLGSS
PSQDVLNRNIIHELPPTNQVLSLHTMLLNKNLNCADFVFYFDRLATILLSWALDDIPV
AHTNIITPGEHTMENVIACQFDQVTAVNIIRSGDCFMKSLRKTIPNITIGKLLIQSDS
QTGEPQLHCEFLPPNIEKFGKVFLMEGQIISGAAMIMAIQVLLDHGIDLEKISVVVYL
ATEVGIRRILNAFDNKVNIFAGMIISREKLQNHQYKWALTRFFDSKYFGCD"
gene complement(<649028..>651712)
/gene="PHO91"
/locus_tag="YNR013C"
/db_xref="GeneID:855747"
mRNA complement(<649028..>651712)
/gene="PHO91"
/locus_tag="YNR013C"
/product="Pho91p"
/transcript_id="NM_001183190.3"
/db_xref="GeneID:855747"
CDS complement(649028..651712)
/gene="PHO91"
/locus_tag="YNR013C"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:17804816]"
/experiment="EXISTENCE:direct assay:GO:1990816
vacuole-mitochondrion membrane contact site
[PMID:25026036]"
/experiment="EXISTENCE:genetic interaction:GO:0005315
phosphate transmembrane transporter activity
[PMID:11779791]"
/experiment="EXISTENCE:genetic interaction:GO:0006817
phosphate ion transport [PMID:11779791]"
/experiment="EXISTENCE:genetic interaction:GO:0015169
glycerol-3-phosphate transmembrane transporter activity
[PMID:20133652]"
/experiment="EXISTENCE:genetic interaction:GO:0015794
glycerol-3-phosphate transmembrane transport
[PMID:20133652]"
/experiment="EXISTENCE:genetic interaction:GO:2000185
regulation of phosphate transmembrane transport
[PMID:12821119]"
/experiment="EXISTENCE:mutant phenotype:GO:0005315
phosphate transmembrane transporter activity
[PMID:12581367]"
/experiment="EXISTENCE:mutant phenotype:GO:0006797
polyphosphate metabolic process [PMID:17804816]"
/experiment="EXISTENCE:mutant phenotype:GO:0015169
glycerol-3-phosphate transmembrane transporter activity
[PMID:20133652]"
/experiment="EXISTENCE:mutant phenotype:GO:0015794
glycerol-3-phosphate transmembrane transport
[PMID:20133652]"
/note="Low-affinity vacuolar phosphate transporter;
exports phosphate from vacuolar lumen to cytosol;
regulates phosphate and polyphosphate metabolism; acts
upstream of Pho81p in regulation of PHO pathway; localizes
to sites of contact between vacuole and mitochondria
(vCLAMPs); targeted to vacuole via AP-3 pathway;
transcription independent of Pi and Pho4p activity;
overexpression results in vigorous growth"
/codon_start=1
/product="Pho91p"
/protein_id="NP_014410.3"
/db_xref="GeneID:855747"
/db_xref="SGD:S000005296"
/translation="MKFSHSLQFNSVPEWSTKYLAYSQLKKLIYSLQKDKLYSNNKHH
VVEPHDANDENLPLLADASPDDQFYISKFVAALNQELKKIDKFYISQETGLIANYNEL
KDDVMELENTNKATQLFNQQQQHQLQSVARNRKSKSQQRQRRFSSVSSTDSNPSLTDM
SIDSAPVIHTQVSNTTNNGNSMQNLASASVSLSNSNPVYLSPFTQHRLSLKKRLISIY
TQLSELKDFIELNQTGFSKICKKFDKSLNTNLKQNYLNYIKFHSHVFNPATINRIQHH
ITETILTYASLNKGTRRPSNTFNLDADRINNDENSSGNEEDEDGNRQEVLDFQDAERE
LSSHLRDHVVWERNTVWKDMMNLERKYQSAKTDNKKFSKLSSSQLRPNANITESMAMS
SGGAGIIAPSTDSLTFRELMHLPPKQWLQFIMGQTSLLKFLLITSCFIALLTFNLTPF
TQDSLQKNCFAILIYASLLWATETIPLFVTSLMIPLLIVVFPVIKDPITSQPMSPRDS
SQFILSTMWSSVIMLLLGGFTLAAALSKYNIAKVLSTHILASAGTNPHFILLTNMFVA
LFVSMWVSNVAAPVLCYSIVQPLLRTLPRNCSYAKALILGIALASNIGGMSSPIASPQ
NIFSIGIMDPSPSWAEWFMIALPVCFICVMAIWVLLIITFPPEPNVKILQLHPSRDPF
TLKQWFVTLVCIITIVLWCLSNQISGIFGEMGIISIIPIVVFFGTGLLTSDDFNNFMW
TIVVLAMGGTTLGKAVSSSGLLSTMAQLIKAQVEHEPIFIIVLIFGLVILVMATFVSH
TVAAMIIVPLMSEIGSNLPSGDHSRLLIVIAALLCSSAMGLPTSGFPNVTAISMIDEV
GDRYLTVGTFITRGVPASLLSYAAIVTVGYGILKVMGF"
gene <652465..>653103
/locus_tag="YNR014W"
/db_xref="GeneID:855748"
mRNA <652465..>653103
/locus_tag="YNR014W"
/product="uncharacterized protein"
/transcript_id="NM_001183191.3"
/db_xref="GeneID:855748"
CDS 652465..653103
/locus_tag="YNR014W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:11914276]"
/note="hypothetical protein; expression is cell-cycle
regulated, Azf1p-dependent, and heat-inducible; YNR014W
has a paralog, YMR206W, that arose from the whole genome
duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014411.3"
/db_xref="GeneID:855748"
/db_xref="SGD:S000005297"
/translation="MSSTDIKPCAVNIPVSAHITFHYKSIADRSSSRSSSSSSCSSAT
SKACSPRGSSVGLPPALSTDNEIVETVLNVSAPVVADPTRPSLFKSNYTAASCLTSDP
TSPSLLPSSRRNSVLPASDFHQCAHHKNFQRRASEPQLPSFDNRSSSEMKRSVSYAQH
SMMFPISDQQEPQTSASPNDHSDPSCPCNRHHHRRNSVAVKFDKPLYERLET"
gene <653387..>654541
/gene="SMM1"
/locus_tag="YNR015W"
/gene_synonym="DUS2"
/db_xref="GeneID:855749"
mRNA <653387..>654541
/gene="SMM1"
/locus_tag="YNR015W"
/gene_synonym="DUS2"
/product="tRNA-dihydrouridine(20) synthase (NAD(+))"
/transcript_id="NM_001183192.1"
/db_xref="GeneID:855749"
CDS 653387..654541
/gene="SMM1"
/locus_tag="YNR015W"
/gene_synonym="DUS2"
/EC_number="1.3.1.91"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006400 tRNA
modification [PMID:12003496]"
/experiment="EXISTENCE:direct assay:GO:0017150 tRNA
dihydrouridine synthase activity [PMID:12003496]"
/experiment="EXISTENCE:genetic interaction:GO:0008033 tRNA
processing [PMID:12559567]"
/experiment="EXISTENCE:mutant phenotype:GO:0006400 tRNA
modification [PMID:14970222]"
/experiment="EXISTENCE:mutant phenotype:GO:0017150 tRNA
dihydrouridine synthase activity [PMID:14970222]"
/note="Dihydrouridine synthase; member of a family of
dihydrouridine synthases including Dus1p, Smm1p, Dus3p,
and Dus4p; modifies uridine residues at position 20 of the
D loop of cytoplasmic pre-tRNAs"
/codon_start=1
/product="tRNA-dihydrouridine(20) synthase (NAD(+))"
/protein_id="NP_014412.1"
/db_xref="GeneID:855749"
/db_xref="SGD:S000005298"
/translation="MVTYAGKLVLAPMVRAGELPTRLMALAHGADLVWSPEIIDKKLI
QCVRKENTALQTVDYVVPSKVQTRPETLVFRTYPKLESSKLIFQIGSASPALATQAAL
KVINDVSGIDINAGCPKHFSIHSGMGSALLRTPDTLCLILKELVKNVGNPHSKPISVK
IRLLDTKQDTLQLVKRLCATGITNLTVHCRKTEMRNREQPITDYIAEIYEICQANNVS
LIVNGAIRDRSHFHDLQANHWKNTNIGGMIAECAERDPTVFDHTSKPSEDGPSWVVAC
REFIQWATKFDNHIGNTKYMLSRIVPGKSVFFQYFARCKSPEEVSFVLKQLNDDGSAQ
TDPSEYLENCRAQEKALKNANAIAKQKRKQTDHIGSDTKKQKVVPLPTDI"
gene complement(<654673..>661374)
/gene="ACC1"
/locus_tag="YNR016C"
/gene_synonym="ABP2; FAS3; MTR7"
/db_xref="GeneID:855750"
mRNA complement(<654673..>661374)
/gene="ACC1"
/locus_tag="YNR016C"
/gene_synonym="ABP2; FAS3; MTR7"
/product="acetyl-CoA carboxylase ACC1"
/transcript_id="NM_001183193.1"
/db_xref="GeneID:855750"
CDS complement(654673..661374)
/gene="ACC1"
/locus_tag="YNR016C"
/gene_synonym="ABP2; FAS3; MTR7"
/EC_number="6.3.4.14"
/EC_number="6.4.1.2"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:9438137]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0003989
acetyl-CoA carboxylase activity [PMID:8098706]"
/experiment="EXISTENCE:mutant phenotype:GO:0004075 biotin
carboxylase activity [PMID:6108218]"
/experiment="EXISTENCE:mutant phenotype:GO:0006606 protein
import into nucleus [PMID:12730220]"
/experiment="EXISTENCE:mutant phenotype:GO:0042759
long-chain fatty acid biosynthetic process [PMID:6103540]"
/note="Acetyl-CoA carboxylase, biotin containing enzyme;
catalyzes carboxylation of cytosolic acetyl-CoA to form
malonyl-CoA and regulates histone acetylation by
regulating the availability of acetyl-CoA; rate-limiting
step for de novo biosynthesis of long-chain fatty acids;
translational regulation in response to nutrients and cell
cycle stage depends on its upstream ORF; ACC1 has a
paralog, HFA1, that arose from the whole genome
duplication"
/codon_start=1
/product="acetyl-CoA carboxylase ACC1"
/protein_id="NP_014413.1"
/db_xref="GeneID:855750"
/db_xref="SGD:S000005299"
/translation="MSEESLFESSPQKMEYEITNYSERHTELPGHFIGLNTVDKLEES
PLRDFVKSHGGHTVISKILIANNGIAAVKEIRSVRKWAYETFGDDRTVQFVAMATPED
LEANAEYIRMADQYIEVPGGTNNNNYANVDLIVDIAERADVDAVWAGWGHASENPLLP
EKLSQSKRKVIFIGPPGNAMRSLGDKISSTIVAQSAKVPCIPWSGTGVDTVHVDEKTG
LVSVDDDIYQKGCCTSPEDGLQKAKRIGFPVMIKASEGGGGKGIRQVEREEDFIALYH
QAANEIPGSPIFIMKLAGRARHLEVQLLADQYGTNISLFGRDCSVQRRHQKIIEEAPV
TIAKAETFHEMEKAAVRLGKLVGYVSAGTVEYLYSHDDGKFYFLELNPRLQVEHPTTE
MVSGVNLPAAQLQIAMGIPMHRISDIRTLYGMNPHSASEIDFEFKTQDATKKQRRPIP
KGHCTACRITSEDPNDGFKPSGGTLHELNFRSSSNVWGYFSVGNNGNIHSFSDSQFGH
IFAFGENRQASRKHMVVALKELSIRGDFRTTVEYLIKLLETEDFEDNTITTGWLDDLI
THKMTAEKPDPTLAVICGAATKAFLASEEARHKYIESLQKGQVLSKDLLQTMFPVDFI
HEGKRYKFTVAKSGNDRYTLFINGSKCDIILRQLSDGGLLIAIGGKSHTIYWKEEVAA
TRLSVDSMTTLLEVENDPTQLRTPSPGKLVKFLVENGEHIIKGQPYAEIEVMKMQMPL
VSQENGIVQLLKQPGSTIVAGDIMAIMTLDDPSKVKHALPFEGMLPDFGSPVIEGTKP
AYKFKSLVSTLENILKGYDNQVIMNASLQQLIEVLRNPKLPYSEWKLHISALHSRLPA
KLDEQMEELVARSLRRGAVFPARQLSKLIDMAVKNPEYNPDKLLGAVVEPLADIAHKY
SNGLEAHEHSIFVHFLEEYYEVEKLFNGPNVREENIILKLRDENPKDLDKVALTVLSH
SKVSAKNNLILAILKHYQPLCKLSSKVSAIFSTPLQHIVELESKATAKVALQAREILI
QGALPSVKERTEQIEHILKSSVVKVAYGSSNPKRSEPDLNILKDLIDSNYVVFDVLLQ
FLTHQDPVVTAAAAQVYIRRAYRAYTIGDIRVHEGVTVPIVEWKFQLPSAAFSTFPTV
KSKMGMNRAVSVSDLSYVANSQSSPLREGILMAVDHLDDVDEILSQSLEVIPRHQSSS
NGPAPDRSGSSASLSNVANVCVASTEGFESEEEILVRLREILDLNKQELINASIRRIT
FMFGFKDGSYPKYYTFNGPNYNENETIRHIEPALAFQLELGRLSNFNIKPIFTDNRNI
HVYEAVSKTSPLDKRFFTRGIIRTGHIRDDISIQEYLTSEANRLMSDILDNLEVTDTS
NSDLNHIFINFIAVFDISPEDVEAAFGGFLERFGKRLLRLRVSSAEIRIIIKDPQTGA
PVPLRALINNVSGYVIKTEMYTEVKNAKGEWVFKSLGKPGSMHLRPIATPYPVKEWLQ
PKRYKAHLMGTTYVYDFPELFRQASSSQWKNFSADVKLTDDFFISNELIEDENGELTE
VEREPGANAIGMVAFKITVKTPEYPRGRQFVVVANDITFKIGSFGPQEDEFFNKVTEY
ARKRGIPRIYLAANSGARIGMAEEIVPLFQVAWNDAANPDKGFQYLYLTSEGMETLKK
FDKENSVLTERTVINGEERFVIKTIIGSEDGLGVECLRGSGLIAGATSRAYHDIFTIT
LVTCRSVGIGAYLVRLGQRAIQVEGQPIILTGAPAINKMLGREVYTSNLQLGGTQIMY
NNGVSHLTAVDDLAGVEKIVEWMSYVPAKRNMPVPILETKDTWDRPVDFTPTNDETYD
VRWMIEGRETESGFEYGLFDKGSFFETLSGWAKGVVVGRARLGGIPLGVIGVETRTVE
NLIPADPANPNSAETLIQEPGQVWHPNSAFKTAQAINDFNNGEQLPMMILANWRGFSG
GQRDMFNEVLKYGSFIVDALVDYKQPIIIYIPPTGELRGGSWVVVDPTINADQMEMYA
DVNARAGVLEPQGMVGIKFRREKLLDTMNRLDDKYRELRSQLSNKSLAPEVHQQISKQ
LADRERELLPIYGQISLQFADLHDRSSRMVAKGVISKELEWTEARRFFFWRLRRRLNE
EYLIKRLSHQVGEASRLEKIARIRSWYPASVDHEDDRQVATWIEENYKTLDDKLKGLK
LESFAQDLAKKIRSDHDNAIDGLSEVIKMLSTDDKEKLLKTLK"
regulatory complement(661704..661715)
/regulatory_class="other"
/note="Upstream open reading frame (uORF) in 5'
untranslated region of ACC1 gene, regulate translation"
gene <662913..>663581
/gene="TIM23"
/locus_tag="YNR017W"
/gene_synonym="MAS6; MIM23; MPI3"
/db_xref="GeneID:855751"
mRNA <662913..>663581
/gene="TIM23"
/locus_tag="YNR017W"
/gene_synonym="MAS6; MIM23; MPI3"
/product="protein transporter TIM23"
/transcript_id="NM_001183194.3"
/db_xref="GeneID:855751"
CDS 662913..663581
/gene="TIM23"
/locus_tag="YNR017W"
/gene_synonym="MAS6; MIM23; MPI3"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:11914276|PMID:14576278|PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0008320 protein
transmembrane transporter activity [PMID:11713477]"
/experiment="EXISTENCE:direct assay:GO:0030943
mitochondrion targeting sequence binding [PMID:8858146]"
/experiment="EXISTENCE:mutant phenotype:GO:0030150 protein
import into mitochondrial matrix
[PMID:10830167|PMID:7600576]"
/experiment="EXISTENCE:mutant phenotype:GO:0030943
mitochondrion targeting sequence binding [PMID:8858146]"
/experiment="EXISTENCE:physical interaction:GO:0005744
TIM23 mitochondrial import inner membrane translocase
complex [PMID:7600576]"
/note="Essential core component of the TIM23 complex;
subunit of the translocase of the inner mitochondrial
membrane involved in protein import into the mitochondrial
matrix and inner membrane; provides a structural role,
contributing to the architecture of the import channel;
functions as a voltage sensor and pre-sequence receptor"
/codon_start=1
/product="protein transporter TIM23"
/protein_id="NP_014414.3"
/db_xref="GeneID:855751"
/db_xref="SGD:S000005300"
/translation="MSWLFGDKTPTDDANAAVGGQDTTKPKELSLKQSLGFEPNINNI
ISGPGGMHVDTARLHPLAGLDKGVEYLDLEEEQLSSLEGSQGLIPSRGWTDDLCYGTG
AVYLLGLGIGGFSGMMQGLQNIPPNSPGKLQLNTVLNHITKRGPFLGNNAGILALSYN
IINSTIDALRGKHDTAGSIGAGALTGALFKSSKGLKPMGYSSAMVAAACAVWCSVKKR
LLEK"
gene <664270..>664944
/gene="RCF2"
/locus_tag="YNR018W"
/gene_synonym="AIM38"
/db_xref="GeneID:855752"
mRNA <664270..>664944
/gene="RCF2"
/locus_tag="YNR018W"
/gene_synonym="AIM38"
/product="Rcf2p"
/transcript_id="NM_001183195.1"
/db_xref="GeneID:855752"
CDS 664270..664944
/gene="RCF2"
/locus_tag="YNR018W"
/gene_synonym="AIM38"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:16823961|PMID:14562095|PMID:14576278|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:22342701]"
/experiment="EXISTENCE:direct assay:GO:0031334 positive
regulation of protein-containing complex assembly
[PMID:38508182]"
/experiment="EXISTENCE:direct assay:GO:0098803 respiratory
chain complex [PMID:22310663|PMID:22342701]"
/experiment="EXISTENCE:genetic interaction:GO:0033617
mitochondrial respiratory chain complex IV assembly
[PMID:22310663]"
/note="Cytochrome c oxidase subunit; has a role in
assembly of respiratory supercomplexes; similar to Rcf1p,
and either Rcf1p or Rcf2p is required for late-stage
assembly of the Cox12p and Cox13p subunits and for
cytochrome c oxidase activity; associates with the
cytochrome c oxidase - cytochrome bc1 supercomplex;
antagonistic with Min8p to control ETC assembly member of
the conserved hypoxia induced gene family; C. elegans
homolog is functional in yeast"
/codon_start=1
/product="Rcf2p"
/protein_id="NP_014415.1"
/db_xref="GeneID:855752"
/db_xref="SGD:S000005301"
/translation="MKILTQDEIEAHRSHTLKGGIEGALAGFAISAIIFKVLPRRYPK
FKPSTLTWSIKTALWITPPTVLTAICAEEASNNFDATMYGSGSSSEDALDEHRRWKSL
STKDKFVEGLSNNKYKIITGAWAASLYGSWVIVNKDPIMTKAQKIVQARMYAQFITVG
LLLASVGLSMYENKLHPNKQKVNEMRRWENALRVAEEEERLEKEGRRTGYVSNEERIN
SKIFKS"
gene <665339..>667267
/gene="ARE2"
/locus_tag="YNR019W"
/gene_synonym="SAT1"
/db_xref="GeneID:855753"
mRNA <665339..>667267
/gene="ARE2"
/locus_tag="YNR019W"
/gene_synonym="SAT1"
/product="sterol acyltransferase"
/transcript_id="NM_001183196.1"
/db_xref="GeneID:855753"
CDS 665339..667267
/gene="ARE2"
/locus_tag="YNR019W"
/gene_synonym="SAT1"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:10672016|PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0008204
ergosterol metabolic process [PMID:10672016]"
/experiment="EXISTENCE:genetic interaction:GO:0034737
ergosterol O-acyltransferase activity [PMID:10672016]"
/experiment="EXISTENCE:mutant phenotype:GO:0008204
ergosterol metabolic process [PMID:10672016]"
/experiment="EXISTENCE:mutant phenotype:GO:0034737
ergosterol O-acyltransferase activity [PMID:10672016]"
/note="Acyl-CoA:sterol acyltransferase; endoplasmic
reticulum enzyme that contributes the major sterol
esterification activity in the presence of oxygen;
contributes to the formation of vacuolar membrane
microdomains after the diauxic shift; ARE2 has a paralog,
ARE1, that arose from the whole genome duplication"
/codon_start=1
/product="sterol acyltransferase"
/protein_id="NP_014416.1"
/db_xref="GeneID:855753"
/db_xref="SGD:S000005302"
/translation="MDKKKDLLENEQFLRIQKLNAADAGKRQSITVDDEGELYGLDTS
GNSPANEHTATTITQNHSVVASNGDVAFIPGTATEGNTEIVTEEVIETDDNMFKTHVK
TLSSKEKARYRQGSSNFISYFDDMSFEHRPSILDGSVNEPFKTKFVGPTLEKEIRRRE
KELMAMRKNLHHRKSSPDAVDSVGKNDGAAPTTVPTAATSETVVTVETTIISSNFSGL
YVAFWMAIAFGAVKALIDYYYQHNGSFKDSEILKFMTTNLFTVASVDLLMYLSTYFVV
GIQYLCKWGVLKWGTTGWIFTSIYEFLFVIFYMYLTENILKLHWLSKIFLFLHSLVLL
MKMHSFAFYNGYLWGIKEELQFSKSALAKYKDSINDPKVIGALEKSCEFCSFELSSQS
LSDQTQKFPNNISAKSFFWFTMFPTLIYQIEYPRTKEIRWSYVLEKICAIFGTIFLMM
IDAQILMYPVAMRALAVRNSEWTGILDRLLKWVGLLVDIVPGFIVMYILDFYLIWDAI
LNCVAELTRFGDRYFYGDWWNCVSWADFSRIWNIPVHKFLLRHVYHSSMSSFKLNKSQ
ATLMTFFLSSVVHELAMYVIFKKLRFYLFFFQMLQMPLVALTNTKFMRNRTIIGNVIF
WLGICMGPSVMCTLYLTF"
gene complement(<667410..>668222)
/gene="ATP23"
/locus_tag="YNR020C"
/db_xref="GeneID:855754"
mRNA complement(<667410..>668222)
/gene="ATP23"
/locus_tag="YNR020C"
/product="putative metalloprotease"
/transcript_id="NM_001183197.3"
/db_xref="GeneID:855754"
CDS complement(667410..668222)
/gene="ATP23"
/locus_tag="YNR020C"
/experiment="EXISTENCE:direct assay:GO:0005758
mitochondrial intermembrane space [PMID:17135288]"
/experiment="EXISTENCE:mutant phenotype:GO:0033615
mitochondrial proton-transporting ATP synthase complex
assembly [PMID:17135288]"
/experiment="EXISTENCE:mutant phenotype:GO:0034982
mitochondrial protein processing
[PMID:17135290|PMID:17135288]"
/note="Putative metalloprotease of the mitochondrial inner
membrane; required for processing of Atp6p; has an
additional role in assembly of the F0 sector of the F1F0
ATP synthase complex; substrate of the Mia40p-Erv1p
disulfide relay system, and folding is assisted by Mia40p"
/codon_start=1
/product="putative metalloprotease"
/protein_id="NP_014417.3"
/db_xref="GeneID:855754"
/db_xref="SGD:S000005303"
/translation="MTMRTNKKNKSSNIKMIRRVRHNLYNQNQGKLIVPNFFFFWHDM
NSSGDNAGFEWWRRTMQYKTGIGLTPEEKTRYEDDSKARELKKECLKCYEYRDWMLKY
SPTVRFMVQAITKLNKGSDSKFDDSKIICDYCPDWKGGGFHPELGILLCQNRLRDKWH
LEDTLSHELIHYFDDLKWQIDWLNLKHHACSEIRASSLSGECRFWEEFKRRGFRTGFH
VARGHQDCVRRRAIISVSGNPNCQSKEHAAKIVDEVWDSCFADTRPFDEIYR"
gene <668377..>669591
/locus_tag="YNR021W"
/db_xref="GeneID:855755"
mRNA <668377..>669591
/locus_tag="YNR021W"
/product="uncharacterized protein"
/transcript_id="NM_001183198.3"
/db_xref="GeneID:855755"
CDS 668377..669591
/locus_tag="YNR021W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095|PMID:26928762]"
/note="hypothetical protein; green fluorescent protein
(GFP)-fusion protein localizes to the endoplasmic
reticulum; YNR021W is not an essential gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014418.3"
/db_xref="GeneID:855755"
/db_xref="SGD:S000005304"
/translation="MSSSIFGPLTGFLERVNSLNAPYQALSYDEQKAMTIWQRVKFYN
WTFELCALGVLFLVYAFYKFGNSVNLKRGNQIFQSLHSFLANDLKFSRVGFNINDSKI
FTVEHQNTWFSSFATGRSAIKSINLNLHLVARSNPFSMCLEYLLGFFFASLKSKQLEE
FMEIVIRPNGILVTSESAHPNKNAHEILTKFRFVTSIVNKEFMNQARTENYFLSIAHT
SENDKLPNNFVYMSDVNQLSGFMFHYSKPYEVLSQAGNLLKYISFTDLPVNPPRDDKE
WESSIEPKAIIRCAVPQNENELKLLNQIISLVVEIYDGFTQDLVQQSPNLFITNDILK
RTTNLRQQELNKIKKFMKETELELAKEKKLELEKAKRRQLKASGQQEKVDQKMKEKRE
RRLKNKQRTRFQ"
gene complement(<669774..>670193)
/gene="MRPL50"
/locus_tag="YNR022C"
/gene_synonym="bL9m"
/db_xref="GeneID:855756"
mRNA complement(<669774..>670193)
/gene="MRPL50"
/locus_tag="YNR022C"
/gene_synonym="bL9m"
/product="mitochondrial 54S ribosomal protein bL9m MRPL50"
/transcript_id="NM_001183199.1"
/db_xref="GeneID:855756"
CDS complement(669774..670193)
/gene="MRPL50"
/locus_tag="YNR022C"
/gene_synonym="bL9m"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:12392552]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:12392552]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005762
mitochondrial large ribosomal subunit [PMID:12392552]"
/note="Mitochondrial ribosomal protein of the large
subunit; not essential for mitochondrial translation"
/codon_start=1
/product="mitochondrial 54S ribosomal protein bL9m MRPL50"
/protein_id="NP_014419.1"
/db_xref="GeneID:855756"
/db_xref="SGD:S000005305"
/translation="MLHCTQVCLSALTKRTHRVKVQVLKDFPRFQLYKGQVANVKPSL
MRNYLHNFNGAKYILSEEHDINTELLKQYQTLEAKLEEDHQQLSKRHETEVQKNMELR
KESVFGHKKEEKPKEEKKGLLDSGITIEEVKIPGLDI"
gene <670418..>672118
/gene="SNF12"
/locus_tag="YNR023W"
/gene_synonym="SWP73"
/db_xref="GeneID:855757"
mRNA <670418..>672118
/gene="SNF12"
/locus_tag="YNR023W"
/gene_synonym="SWP73"
/product="Snf12p"
/transcript_id="NM_001183200.1"
/db_xref="GeneID:855757"
CDS 670418..672118
/gene="SNF12"
/locus_tag="YNR023W"
/gene_synonym="SWP73"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0016514 SWI/SNF
complex [PMID:8127913|PMID:18644858]"
/experiment="EXISTENCE:genetic interaction:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:8804308]"
/experiment="EXISTENCE:mutant phenotype:GO:0000785
chromatin [PMID:28249159]"
/experiment="EXISTENCE:mutant phenotype:GO:0005198
structural molecule activity [PMID:28249159]"
/experiment="EXISTENCE:mutant phenotype:GO:0006338
chromatin remodeling [PMID:28249159]"
/experiment="EXISTENCE:mutant phenotype:GO:0034198
cellular response to amino acid starvation
[PMID:10549298]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:10549298|PMID:8816487]"
/note="73 kDa subunit of the SWI/SNF chromatin remodeling
complex; involved in transcriptional regulation; relocates
to the cytosol under hypoxic conditions; deletion mutants
are temperature-sensitive; SNF12 has a paralog, RSC6, that
arose from the whole genome duplication"
/codon_start=1
/product="Snf12p"
/protein_id="NP_014420.1"
/db_xref="GeneID:855757"
/db_xref="SGD:S000005306"
/translation="MSKVMKPSNGKGSRKSSKAATPDTKNFFHAKKKDPVNQDKANNA
SQITPTVPHSHPSDMVIPDHLAELIPELYSFQQLVDSEKRLDHFIHLRNLHMKRMVAQ
WERSKLSQEFLYPHLNFPNVKFLRIFISNVSENQPWQMDTNNEADLMALENATWTMRI
EGRLLDNVQANDPAREKFSSFIESIVVDFKNKENDNVPSTKFNAAPEENATEGPSDKK
LNLNLPLQFSLPNGDNSTTTNTDQNNATMGEETAKKDMSSTTPKLESVKWQYDPNNPV
DFDGLDIKRVGSENVECTISILRKSSPEEPFMSYSPQLTAIIGLKSGTSHDAIFSIYK
YIHLNELLTNDESAFENLMGNRNNHNSNTSTSKMLDAASSQVSIVKLDTQLITLLPSS
LKESSPDTMKLTDLLSLINSTHLLPLQPIEIDYTVRVDKASTYGELVLDIEVPDVNAL
KFNNTQRESQIGAAELNENARELEQIKPKIALQDKEITSVLSNLHESNKRYRFFKKIS
EDPVKALNECIASTSNALKVLSGDEGYNEDMVRRANFYKENEAMLRENIEVILSNGRM
"
gene <672409..>672969
/gene="MPP6"
/locus_tag="YNR024W"
/db_xref="GeneID:855758"
mRNA <672409..>672969
/gene="MPP6"
/locus_tag="YNR024W"
/product="Mpp6p"
/transcript_id="NM_001183201.3"
/db_xref="GeneID:855758"
CDS 672409..672969
/gene="MPP6"
/locus_tag="YNR024W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095|PMID:18591258|PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0008266 poly(U) RNA
binding [PMID:18591258]"
/experiment="EXISTENCE:direct assay:GO:0044877
protein-containing complex binding
[PMID:18591258|PMID:19046973]"
/experiment="EXISTENCE:genetic interaction:GO:0071030
nuclear mRNA surveillance of spliceosomal pre-mRNA
splicing [PMID:18591258]"
/experiment="EXISTENCE:genetic interaction:GO:0071031
nuclear mRNA surveillance of mRNA 3'-end processing
[PMID:18591258]"
/experiment="EXISTENCE:genetic interaction:GO:0071035
nuclear polyadenylation-dependent rRNA catabolic process
[PMID:18591258]"
/experiment="EXISTENCE:genetic interaction:GO:0071039
nuclear polyadenylation-dependent CUT catabolic process
[PMID:18591258]"
/experiment="EXISTENCE:mutant phenotype:GO:0000467
exonucleolytic trimming to generate mature 3'-end of 5.8S
rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S
rRNA, LSU-rRNA) [PMID:18591258]"
/experiment="EXISTENCE:mutant phenotype:GO:0071039 nuclear
polyadenylation-dependent CUT catabolic process
[PMID:18591258]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:27076633]"
/note="Nuclear exosome-associated RNA binding protein;
involved in surveillance of pre-rRNAs and pre-mRNAs, and
the degradation of cryptic non-coding RNAs (ncRNA);
copurifies with ribosomes; relocalizes to the cytosol in
response to hypoxia"
/codon_start=1
/product="Mpp6p"
/protein_id="NP_014421.3"
/db_xref="GeneID:855758"
/db_xref="SGD:S000005307"
/translation="MSANNGVTGKLSSRVMNMKFMKFGKTDDEESSNSNTPSNINSDV
EPIEQKGKLFGLDDSAWDLNSYKDDLKKISGKEKKKVKRVVYKKRPNLIISNVGYSEL
RKPEGVISGRKTFGDNSDDSGSRKRKFDEGEQNEDEKRDAKDKEFTGSQDDGEDEYDL
DKLFKDSIKKKKTNHNGKNKNRNSKK"
gene complement(<673274..>674689)
/gene="SEC12"
/locus_tag="YNR026C"
/gene_synonym="SED2"
/db_xref="GeneID:855760"
mRNA complement(<673274..>674689)
/gene="SEC12"
/locus_tag="YNR026C"
/gene_synonym="SED2"
/product="Sar family guanine nucleotide exchange factor
SEC12"
/transcript_id="NM_001183203.1"
/db_xref="GeneID:855760"
CDS complement(673274..674689)
/gene="SEC12"
/locus_tag="YNR026C"
/gene_synonym="SED2"
/experiment="EXISTENCE:direct assay:GO:0003400 regulation
of COPII vesicle coating [PMID:8377826]"
/experiment="EXISTENCE:direct assay:GO:0005085
guanyl-nucleotide exchange factor activity
[PMID:8583945|PMID:8377826]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:8707815|PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:3047151]"
/experiment="EXISTENCE:mutant phenotype:GO:0005085
guanyl-nucleotide exchange factor activity [PMID:8377826]"
/note="Guanine nucleotide exchange factor (GEF); activates
Sar1p by catalyzing the exchange of GDP for GTP; required
for the initiation of COPII vesicle formation in ER to
Golgi transport; glycosylated integral membrane protein of
the ER; SEC12 has a paralog, SED4, that arose from the
whole genome duplication"
/codon_start=1
/product="Sar family guanine nucleotide exchange factor
SEC12"
/protein_id="NP_014423.1"
/db_xref="GeneID:855760"
/db_xref="SGD:S000005309"
/translation="MKFVTASYNVGYPAYGAKFLNNDTLLVAGGGGEGNNGIPNKLTV
LRVDPTKDTEKEQFHILSEFALEDNDDSPTAIDASKGIILVGCNENSTKITQGKGNKH
LRKFKYDKVNDQLEFLTSVDFDASTNADDYTKLVYISREGTVAAIASSKVPAIMRIID
PSDLTEKFEIETRGEVKDLHFSTDGKVVAYITGSSLEVISTVTGSCIARKTDFDKNWS
LSKINFIADDTVLIAASLKKGKGIVLTKISIKSGNTSVLRSKQVTNRFKGITSMDVDM
KGELAVLASNDNSIALVKLKDLSMSKIFKQAHSFAITEVTISPDSTYVASVSAANTIH
IIKLPLNYANYTSMKQKISKFFTNFILIVLLSYILQFSYKHNLHSMLFNYAKDNFLTK
RDTISSPYVVDEDLHQTTLFGNHGTKTSVPSVDSIKVHGVHETSSVNGTEVLCTESNI
INTGGAEFEITNATFREIDDA"
gene <674923..>675876
/gene="BUD17"
/locus_tag="YNR027W"
/db_xref="GeneID:855761"
mRNA <674923..>675876
/gene="BUD17"
/locus_tag="YNR027W"
/product="putative pyridoxal kinase BUD17"
/transcript_id="NM_001183204.1"
/db_xref="GeneID:855761"
CDS 674923..675876
/gene="BUD17"
/locus_tag="YNR027W"
/EC_number="2.7.1.35"
/experiment="EXISTENCE:curator inference:GO:0042823
pyridoxal phosphate biosynthetic process [PMID:9099727]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="Putative pyridoxal kinase; a key enzyme in vitamin
B6 metabolism; involved in bud-site selection; diploid
mutants display a random rather than a bipolar budding
pattern; similarity to yeast BUD16 and human pyridoxal
kinase (PDXK)"
/codon_start=1
/product="putative pyridoxal kinase BUD17"
/protein_id="NP_014424.1"
/db_xref="GeneID:855761"
/db_xref="SGD:S000005310"
/translation="MTSTLHTTKKVLSIQSHVIHGYVGNKAATFPLQYRGWDVDVLNT
VQFSNHSGYAHFTGFKCSTEELVDIVEKGLIGSLRIKYDAVLSGYLPNVQALQKVAGI
VGQLCEGSENVKWILDPVLGDNGRLYVDRECVAVYQDILQNFKIFLATPNQFEMELLV
GMSIRTLDDAKQAFKLFHKKYPRVSRIVVTSLELSEFLSNDTYVVAGFDCSASEEIFF
YEIPKINAKFSGSGDLISAMLTDSLLGDRRCTQLSLSASLGQVLWLVTSILQKTYDLN
IAERGPQDSTIDIKDLKLIQCRDILKQDLIPSIGKPKTIKI"
gene <676177..>677103
/gene="CPR8"
/locus_tag="YNR028W"
/db_xref="GeneID:855762"
mRNA <676177..>677103
/gene="CPR8"
/locus_tag="YNR028W"
/product="peptidylprolyl isomerase family protein CPR8"
/transcript_id="NM_001183205.3"
/db_xref="GeneID:855762"
CDS 676177..677103
/gene="CPR8"
/locus_tag="YNR028W"
/EC_number="5.2.1.8"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:14562095]"
/note="Peptidyl-prolyl cis-trans isomerase (cyclophilin);
catalyzes the cis-trans isomerization of peptide bonds
N-terminal to proline residues; potential role in the
secretory pathway; CPR8 has a paralog, CPR4, that arose
from the whole genome duplication"
/codon_start=1
/product="peptidylprolyl isomerase family protein CPR8"
/protein_id="NP_014425.3"
/db_xref="GeneID:855762"
/db_xref="SGD:S000005311"
/translation="MKSFFLYLYVAFMFSCITALPLPVDNKRASSDSLDLKKKYAPDP
PITHNVNIGIVFTDPESSEEAGRLITIDLYGTMVPKTVMTFCQYVDSVKDRLASRHSY
SPERDFDKILPNGAIEGSSVSSSSIEETEMLAPKLPEENHSLIHDRPGRVSMIKDDKG
LKFIIETSETPLEGESVVFGQVTAGLKDLMDKLANVKTDENGKPEQPITIGYISSQEH
RIQHAKEAHEKYLQRLQDYQNGDLEKGITLKNYLYQGSQRKLEDAKYNQLHHPLPKIM
LGISVLLLFYVLAKYRKRIFNRSSKIVSIRED"
gene complement(<677199..>678488)
/gene="ZNG1"
/locus_tag="YNR029C"
/db_xref="GeneID:855763"
mRNA complement(<677199..>678488)
/gene="ZNG1"
/locus_tag="YNR029C"
/product="GTP-dependent zinc transferase"
/transcript_id="NM_001183206.3"
/db_xref="GeneID:855763"
CDS complement(677199..678488)
/gene="ZNG1"
/locus_tag="YNR029C"
/experiment="EXISTENCE:direct assay:GO:0003924 GTPase
activity [PMID:35584675]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008047 enzyme
activator activity [PMID:35584675]"
/experiment="EXISTENCE:direct assay:GO:0140827 zinc
chaperone activity [PMID:35584675]"
/experiment="EXISTENCE:genetic interaction:GO:0034224
cellular response to zinc ion starvation [PMID:35584675]"
/experiment="EXISTENCE:genetic interaction:GO:0051604
protein maturation [PMID:35584675]"
/experiment="EXISTENCE:mutant phenotype:GO:0034224
cellular response to zinc ion starvation [PMID:35584675]"
/experiment="EXISTENCE:mutant phenotype:GO:0051604 protein
maturation [PMID:35584675]"
/note="GTP-dependent zinc transferase; couples GTP
hydrolysis to the activation of methionine aminopeptidase
Map1p, especially during zinc starvation; deletion confers
reduced fitness in saline; contains GTPase sequence motifs
and a CxCC metal-binding motif"
/codon_start=1
/product="GTP-dependent zinc transferase"
/protein_id="NP_014426.3"
/db_xref="GeneID:855763"
/db_xref="SGD:S000005312"
/translation="MSALRNIKFNEEEDGELPCLVTGEENNLQEILENVSYDGGNIVS
DAKVERVNKQVENTSAGATDVHEKKRIPVSIITGYLGSGKSTLLEKIALKGADKKIAV
ILNEFGDSSEIEKAMTIKNGSNSYQEWLDLGNGCLCCSLKNIGVKAIEDMVERSPGKI
DYILLETSGIADPAPIAKMFWQDEGLNSSVYIDGIITVLDCEHILKCLDDISIDAHWH
GDKVGLEGNLTIAHFQLAMADRIIMNKYDTIEHSPEMVKQLKERVREINSIAPMFFTK
YSDTPIQNLLDIHAYDSVRISDILDSGSGNGTIHDDRMGTIMLTFRPLKNEEEYNNKF
IKQFLQPLLWKNFGAMTVLGGRRRDDGRDWEVQRTKGLILIEGENPIARVIQGVRDTY
DVFPGKYDGSNKECKIVLIGKYLEKESIEELLRKTLE"
gene <678799..>680454
/gene="ALG12"
/locus_tag="YNR030W"
/gene_synonym="ECM39"
/db_xref="GeneID:855764"
mRNA <678799..>680454
/gene="ALG12"
/locus_tag="YNR030W"
/gene_synonym="ECM39"
/product="dolichyl-P-Man:Man(7)GlcNAc(2)-PP-dolichol
alpha-1,6-mannosyltransferase"
/transcript_id="NM_001183207.1"
/db_xref="GeneID:855764"
CDS 678799..680454
/gene="ALG12"
/locus_tag="YNR030W"
/gene_synonym="ECM39"
/EC_number="2.4.1.260"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095|PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0000009
alpha-1,6-mannosyltransferase activity [PMID:10336995]"
/experiment="EXISTENCE:mutant phenotype:GO:0006488
dolichol-linked oligosaccharide biosynthetic process
[PMID:10336995]"
/experiment="EXISTENCE:mutant phenotype:GO:0009101
glycoprotein biosynthetic process
[PMID:12460938|PMID:10336995]"
/note="Alpha-1,6-mannosyltransferase localized to the ER;
responsible for addition of alpha-1,6 mannose to
dolichol-linked Man7GlcNAc2; acts in the dolichol pathway
for N-glycosylation; human homolog ALG12 complements yeast
null mutant"
/codon_start=1
/product="dolichyl-P-Man:Man(7)GlcNAc(2)-PP-dolichol
alpha-1,6-mannosyltransferase"
/protein_id="NP_014427.1"
/db_xref="GeneID:855764"
/db_xref="SGD:S000005313"
/translation="MRWSVLDTVLLTVISFHLIQAPFTKVEESFNIQAIHDILTYSVF
DISQYDHLKFPGVVPRTFVGAVIIAMLSRPYLYLSSLIQTSRPTSIDVQLVVRGIVGL
TNGLSFIYLKNCLQDMFDEITEKKKEENEDKDIYIYDSAGTWFLLFLIGSFHLMFYST
RTLPNFVMTLPLTNVALGWVLLGRYNAAIFLSALVAIVFRLEVSALSAGIALFSVIFK
KISLFDAIKFGIFGLGLGSAISITVDSYFWQEWCLPEVDGFLFNVVAGYASKWGVEPV
TAYFTHYLRMMFMPPTVLLLNYFGYKLAPAKLKIVSLASLFHIIVLSFQPHKEWRFII
YAVPSIMLLGATGAAHLWENMKVKKITNVLCLAILPLSIMTSFFISMAFLYISRMNYP
GGEALTSFNDMIVEKNITNATVHISIPPCMTGVTLFGELNYGVYGINYDKTENTTLLQ
EMWPSFDFLITHEPTASQLPFENKTTNHWELVNTTKMFTGFDPTYIKNFVFQERVNVL
SLLKQIIFDKTPTVFLKELTANSIVKSDVFFTYKRIKQDEKTD"
gene complement(<680694..>685433)
/gene="SSK2"
/locus_tag="YNR031C"
/db_xref="GeneID:855765"
mRNA complement(<680694..>685433)
/gene="SSK2"
/locus_tag="YNR031C"
/product="mitogen-activated protein kinase kinase kinase
SSK2"
/transcript_id="NM_001183208.1"
/db_xref="GeneID:855765"
CDS complement(680694..685433)
/gene="SSK2"
/locus_tag="YNR031C"
/EC_number="2.7.11.25"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:12857882]"
/experiment="EXISTENCE:direct assay:GO:0003779 actin
binding [PMID:12181352]"
/experiment="EXISTENCE:direct assay:GO:0004709 MAP kinase
kinase kinase activity [PMID:9482735]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:12181352]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:12857882]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:12181352|PMID:12857882]"
/experiment="EXISTENCE:direct assay:GO:0005938 cell cortex
[PMID:12857882|PMID:12181352]"
/experiment="EXISTENCE:direct assay:GO:0071474 cellular
hyperosmotic response [PMID:9482735|PMID:12181352]"
/experiment="EXISTENCE:genetic interaction:GO:0007234
osmosensory signaling via phosphorelay pathway
[PMID:9482735]"
/experiment="EXISTENCE:genetic interaction:GO:0032956
regulation of actin cytoskeleton organization
[PMID:12857882]"
/experiment="EXISTENCE:genetic interaction:GO:0038066
p38MAPK cascade [PMID:7624781|PMID:9742096]"
/experiment="EXISTENCE:genetic interaction:GO:0071474
cellular hyperosmotic response [PMID:12857882]"
/experiment="EXISTENCE:mutant phenotype:GO:0032956
regulation of actin cytoskeleton organization
[PMID:12181352|PMID:12857882]"
/experiment="EXISTENCE:mutant phenotype:GO:0038066 p38MAPK
cascade [PMID:7624781]"
/experiment="EXISTENCE:mutant phenotype:GO:0071474
cellular hyperosmotic response
[PMID:12857882|PMID:12181352]"
/experiment="EXISTENCE:physical interaction:GO:0007234
osmosensory signaling via phosphorelay pathway
[PMID:9482735]"
/note="MAP kinase kinase kinase of HOG1 mitogen-activated
signaling pathway; interacts with Ssk1p, leading to
autophosphorylation and activation of Ssk2p which
phosphorylates Pbs2p; also mediates actin cytoskeleton
recovery from osmotic stress; a HOG-independent function
of Ssk2p mediates the calcium-sensitive phenotype of the
ptp2 msg5 double disruptant; SSK2 has a paralog, SSK22,
that arose from the whole genome duplication"
/codon_start=1
/product="mitogen-activated protein kinase kinase kinase
SSK2"
/protein_id="NP_014428.1"
/db_xref="GeneID:855765"
/db_xref="SGD:S000005314"
/translation="MSHSDYFNYKPYGDSTEKPSSSKMRQSSSSSSSRLRSESLGRNS
NTTQARVASSPISPGLHSTQYFRSPNAVYSPGESPLNTVQLFNRLPGIPQGQFFHQNA
ISGSSSSSARSSRRPSNIGLPLPKNPQQSLPKLSTQPVPVHKKVEASKTESEIIKKPA
PVNSNQDPLLTTPTLVISPELASLNTTNTSIMSTPQNITNQTSNKHIPTRSQPNGSTS
SSTLQDIVTTNSSQRSVGHHGGSTTSLRTYKKQYVLNEQLYLRKMRNRANDDYYTRGI
VASSNFEDDEENFSNKGEDDLELEMDDLLKVEGEDKDNDFNFGYNFITSSTKNNENVV
SMSLNYLKGKLDWLRDVNNDQPCEIEDEEWHSILGSEDLLSKLLQNPMVNNRFEWQTM
LSKVLKGDIVRNEKTKIANQGKGPGFNTQFSDDIWIELKAWMNGRTVEDQNKSLRIFR
DSTDSVFQEIMAFKLEDNMSADEAAETIKSLVDKYYRVLNLWPNIKRMHAEKPITKTE
AFRNRIDTLNSWLNFKFNFDTNIAYLKKWIVGNKELESTTEVDNTTVNLDDPAVFATN
CKRFAEQIMKEKDIELIFQKKIFFPLAPWILKAKFFFLKYQKTWNELNLSYLDQDLEF
LLMFPMRLVKDIILIRLSYAKKIQNPTLMMIDQMMDDFSTYIKLAVQMKFTVASYCND
WFFKVKIDPEFDHTVVEGLEYFFSILELRILYSGKNSFKTSKEPDLLLKYWEMFRNVG
YYIDDAGELIAAEFTKLTLRLVHRLHAYLLRQQNTPPKLENEAAAEKWLVQIFEILGS
MKRKLNRFTNILTKAFQNFVRYKIEDHNYLLKQLKETGHFLIYTGGYLEQNGTYLIGS
PELLGCKDDDILRIIKNSDIGCDLVPKLEINNSLTIYNALDDNWNSNSSLGSDISNDG
TPFYYIKNDLTTQPRSYNGNRVNREPDFENSRSTEEEFYELETRLNSLGYVLVLTPQE
PLLWEGEMYNLSDNKTIKPEGLNLKVIPNSIDLMCQGSSYALEYQCDRFQQISGSSVS
FLEKKSSSETVKNNLQRINKAYFRCTYSVLKNYTKIVTTFKKVSPVNDLLNNIFLFGR
DFGLNFLRINVANNEKRSIIILLMMRLSIGWLKFLAEDCDPTDQRVFRWCVTSMEFAM
HMVSGWNILALDECQFSSLKQKISECMSLLISHFDIIGARSIEVEKINQQARSNLDLE
DVFDDDMMLQVNSEFRVQSIMELEERIKRNPHQTGKVIDDSDKGNKYLVSLASSISNV
SMRWQKRNFIGGGTFGRVYSAVDLDNGEILAVKEINIQDSKSMQKIFPLIKEEMSVLE
ILNHPNIVSYYGVEVHRDKVNIFMEYCEGGSLAALLEHGRIEDEMVTQVYTLQLLEGL
AYLHESGIVHRDVKPENILLDFNGVIKYVDFGAAKKIANNGTRLASMNKIENADGEHE
DVTHVSDSKAVKNNENALLDMMGTPMYMAPESITGSTTKGKLGADDVWSLGCVVLEMI
TGRRPWANLDNEWAIMYHVAAGHTPQFPTKDEVSSAGMKFLERCLIQNPSKRASAVEL
LMDPWIVQIREIAFGDDSSSTDTEERE"
gene <686010..>687116
/gene="PPG1"
/locus_tag="YNR032W"
/db_xref="GeneID:855766"
mRNA <686010..>687116
/gene="PPG1"
/locus_tag="YNR032W"
/product="putative serine/threonine-protein kinase PPG1"
/transcript_id="NM_001183209.3"
/db_xref="GeneID:855766"
CDS 686010..687116
/gene="PPG1"
/locus_tag="YNR032W"
/EC_number="3.1.3.16"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:33317697]"
/experiment="EXISTENCE:mutant phenotype:GO:0004722 protein
serine/threonine phosphatase activity [PMID:33317697]"
/experiment="EXISTENCE:mutant phenotype:GO:0005977
glycogen metabolic process [PMID:7678255]"
/experiment="EXISTENCE:mutant phenotype:GO:1901525
negative regulation of mitophagy [PMID:33317697]"
/note="Serine protein phosphatase involved in formation of
FAR complex; inhibits mitophagy by dephosphorylating
Atg32p; required for glycogen accumulation; interacts with
Tap42p, which binds to and regulates other protein
phosphatases"
/codon_start=1
/product="putative serine/threonine-protein kinase PPG1"
/protein_id="NP_014429.3"
/db_xref="GeneID:855766"
/db_xref="SGD:S000005315"
/translation="MELDECLERLYKAQLLPEVTVRALCFKLKEMLVKESNVIHIQTP
VTVVGDMHGQFHDMLEIFQIGGPVPDTNYLFLGDYVDRGLYSVETIMLLIVLKLRYPS
RIHLLRGNHESRQITQSYGFYTECLNKYGGNSRVWQYLTDIFDYLVLCCIIDDEIFCV
HGGLSPNVQTIDQIKIIDRFREIPHDGAMADLVWSDPEENNNPTLDHPDNSGQHFQVS
PRGAGYTFGRSVVEKFLRMNDMNRIYRAHQLCNEGYQIYFDGLVTTVWSAPNYCYRCG
NKASILELYSKDQFYFNVFEEAPENKLLKENSMNDNALEDSISNPVANRKLIADYFED
DSASADGSTDPEMYIFSDVYQARSASNRHVDYFL"
gene complement(<687243..>687464)
/gene="HUB1"
/locus_tag="YNR032C-A"
/db_xref="GeneID:855767"
mRNA complement(<687243..>687464)
/gene="HUB1"
/locus_tag="YNR032C-A"
/product="ubiquitin-like protein HUB1"
/transcript_id="NM_001184344.1"
/db_xref="GeneID:855767"
CDS complement(687243..687464)
/gene="HUB1"
/locus_tag="YNR032C-A"
/experiment="EXISTENCE:direct assay:GO:0031386 protein tag
activity [PMID:11923536]"
/experiment="EXISTENCE:direct assay:GO:0043687
post-translational protein modification [PMID:11923536]"
/experiment="EXISTENCE:mutant phenotype:GO:0000753 cell
morphogenesis involved in conjugation with cellular fusion
[PMID:11923536]"
/experiment="EXISTENCE:mutant phenotype:GO:0033120
positive regulation of RNA splicing [PMID:31666190]"
/experiment="EXISTENCE:physical interaction:GO:0000398
mRNA splicing, via spliceosome [PMID:15620657]"
/note="Ubiquitin-like protein modifier; promotes
alternative splicing of SRC1 pre-mRNA; binds
non-covalently to the HIND domain of Snu66, may function
in modification of Sph1p and Hbt1p, functionally
complemented by the human or S. pombe ortholog; mechanism
of Hub1p adduct formation not yet clear"
/codon_start=1
/product="ubiquitin-like protein HUB1"
/protein_id="NP_014430.1"
/db_xref="GeneID:855767"
/db_xref="SGD:S000007251"
/translation="MIEVVVNDRLGKKVRVKCLAEDSVGDFKKVLSLQIGTQPNKIVL
QKGGSVLKDHISLEDYEVHDQTNLELYYL"
gene <687635..>689998
/gene="ABZ1"
/locus_tag="YNR033W"
/db_xref="GeneID:855768"
mRNA <687635..>689998
/gene="ABZ1"
/locus_tag="YNR033W"
/product="4-amino-4-deoxychorismate synthase"
/transcript_id="NM_001183210.1"
/db_xref="GeneID:855768"
CDS 687635..689998
/gene="ABZ1"
/locus_tag="YNR033W"
/EC_number="2.6.1.85"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0008153
4-aminobenzoate biosynthetic process [PMID:8346682]"
/experiment="EXISTENCE:mutant phenotype:GO:0046820
4-amino-4-deoxychorismate synthase activity
[PMID:8346682]"
/note="Para-aminobenzoate (PABA) synthase; has similarity
to Escherichia coli PABA synthase components PabA and
PabB; required for the synthesis of para-aminobenzoic
acid, an important intermediate for folate and ubiquinone
Q biosynthesis; protein abundance increases in response to
DNA replication stress"
/codon_start=1
/product="4-amino-4-deoxychorismate synthase"
/protein_id="NP_014431.1"
/db_xref="GeneID:855768"
/db_xref="SGD:S000005316"
/translation="MLSDTIDTKQQQQQLHVLFIDSYDSFTYNVVRLIEQQTDISPGV
NAVHVTTVHSDTFQSMDQLLPLLPLFDAIVVGPGPGNPNNGAQDMGIISELFENANGK
LDEVPILGICLGFQAMCLAQGADVSELNTIKHGQVYEMHLNDAARACGLFSGYPDTFK
STRYHSLHVNAEGIDTLLPLCTTEDENGILLMSAQTKNKPWFGVQYHPESCCSELGGL
LVSNFLKLSFINNVKTGRWEKKKLNGEFSDILSRLDRTIDRDPIYKVKEKYPKGEDTT
YVKQFEVSEDPKLTFEICNIIREEKFVMSSSVISENTGEWSIIALPNSASQVFTHYGA
MKKTTVHYWQDSEISYTLLKKCLDGQDSDLPGSLEVIHEDKSQFWITLGKFMENKIID
NHREIPFIGGLVGILGYEIGQYIACGRCNDDENSLVPDAKLVFINNSIVINHKQGKLY
CISLDNTFPVALEQSLRDSFVRKKNIKQSLSWPKYLPEEIDFIITMPDKLDYAKAFKK
CQDYMHKGDSYEMCLTTQTKVVPSAVIEPWRIFQTLVQRNPAPFSSFFEFKDIIPRQD
ETPPVLCFLSTSPERFLKWDADTCELRPIKGTVKKGPQMNLAKATRILKTPKEFGENL
MILDLIRNDLYELVPDVRVEEFMSVQEYATVYQLVSVVKAHGLTSASKKTRYSGIDVL
KHSLPPGSMTGAPKKITVQLLQDKIESKLNKHVNGGARGVYSGVTGYWSVNSNGDWSV
NIRCMYSYNGGTSWQLGAGGAITVLSTLDGELEEMYNKLESNLQIFM"
gene <690321..>691286
/gene="SOL1"
/locus_tag="YNR034W"
/db_xref="GeneID:855769"
mRNA <690321..>691286
/gene="SOL1"
/locus_tag="YNR034W"
/product="Sol1p"
/transcript_id="NM_001183211.3"
/db_xref="GeneID:855769"
CDS 690321..691286
/gene="SOL1"
/locus_tag="YNR034W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:15454531|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0006409 tRNA
export from nucleus [PMID:15454531]"
/note="Protein with a possible role in tRNA export; shows
similarity to 6-phosphogluconolactonase non-catalytic
domains but does not exhibit this enzymatic activity;
homologous to Sol3p and Sol4p; SOL1 has a paralog, SOL2,
that arose from the whole genome duplication; protein
abundance increases in response to DNA replication stress"
/codon_start=1
/product="Sol1p"
/protein_id="NP_014432.3"
/db_xref="GeneID:855769"
/db_xref="SGD:S000005317"
/translation="MTTTVPKVFAFHEFAGVAEAVADHVIHAQNSALKKGKVSRSTQM
SGTSLNGNGNTESKTMERVNSVRSNASSRGGSEDGATKKLKKEKERRFKIALSGGSLI
QVLHEGLLKRDDVQWGKWDIYFADERLVPFSSSESNYGLAKRKIFDLIDTEKYGTPKI
YHIDESLINDPQECADNYEKILIKGFAGRDSVKLPMFDLFLLGCAPDGHIASLFPNFQ
ENLRENLAWVIPVENAPSGPSNRISLTIPVICHSHRVTFVVEGATKAPVIKTIMERPE
KGLPSSIVNEGAAGRVSWFVDDDALKDVFVIKKKYKFYDDENLTE"
rep_origin 691486..691730
/note="ARS1427; Autonomously Replicating Sequence"
/db_xref="SGD:S000118393"
gene <692561..>692857
/gene="EGO4"
/locus_tag="YNR034W-A"
/db_xref="GeneID:855770"
mRNA <692561..>692857
/gene="EGO4"
/locus_tag="YNR034W-A"
/product="Ego4p"
/transcript_id="NM_001184446.3"
/db_xref="GeneID:855770"
CDS 692561..692857
/gene="EGO4"
/locus_tag="YNR034W-A"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26206314]"
/note="hypothetical protein; expressed during diauxic
shift and stationary phase, and negatively regulated by
glucose; expression is regulated by Msn2p/Msn4p;
overexpression slows down progression through meiosis and
improves fermentative efficiency; YNR034W-A has a paralog,
YCR075W-A, that arose from the whole genome duplication"
/codon_start=1
/product="Ego4p"
/protein_id="NP_061494.3"
/db_xref="GeneID:855770"
/db_xref="SGD:S000007525"
/translation="MKSSIPITEVLPRAVGSLTFDENYNLLDTSGVAKVIEKSPIAEI
IRKSNAELGRLGYSVYEDAQYIGHAFKKAGHFIVYFTPKNKNREGVVPPVGITN"
gene complement(<693019..>694047)
/gene="ARC35"
/locus_tag="YNR035C"
/gene_synonym="END9"
/db_xref="GeneID:855771"
mRNA complement(<693019..>694047)
/gene="ARC35"
/locus_tag="YNR035C"
/gene_synonym="END9"
/product="Arc35p"
/transcript_id="NM_001183212.1"
/db_xref="GeneID:855771"
CDS complement(693019..694047)
/gene="ARC35"
/locus_tag="YNR035C"
/gene_synonym="END9"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:14554195]"
/experiment="EXISTENCE:direct assay:GO:0005885 Arp2/3
protein complex [PMID:10377407|PMID:9210376]"
/experiment="EXISTENCE:mutant phenotype:GO:0000226
microtubule cytoskeleton organization [PMID:14554195]"
/experiment="EXISTENCE:mutant phenotype:GO:0006898
receptor-mediated endocytosis [PMID:7929582]"
/experiment="EXISTENCE:mutant phenotype:GO:0030036 actin
cytoskeleton organization [PMID:14554195]"
/experiment="EXISTENCE:mutant phenotype:GO:0044396 actin
cortical patch organization [PMID:10377407]"
/experiment="EXISTENCE:mutant phenotype:GO:0060090
molecular adaptor activity [PMID:10377407]"
/experiment="EXISTENCE:mutant phenotype:GO:0072697 protein
localization to cell cortex [PMID:10749918]"
/experiment="EXISTENCE:physical interaction:GO:0005516
calmodulin binding [PMID:10749918]"
/note="Subunit of the ARP2/3 complex; ARP2/3 is required
for the motility and integrity of cortical actin patches;
required for cortical localization of calmodulin"
/codon_start=1
/product="Arc35p"
/protein_id="NP_014433.1"
/db_xref="GeneID:855771"
/db_xref="SGD:S000005318"
/translation="MLHLQPQNLLIQKTLNEAIEALRKGSPLTMDRIVSDFDYTTYHI
SNTAEDKSILLLSVKTKAWVSVSECQLDGSLTLLKFLADHYSSLGGVTIPSEVEPGYD
YTLQITLAELVQESILQLSVLKTIILSFPFELAISKFIELSQQQPAPVEAEITGGEVA
ANGDNTLFTIKYRDEENIFIKPSNDRVTIIFETIFQDETDKIFGKVFLQEFVDARKRN
RQIQSAPQVLYSHEPPLELKRLYQPPKVAEQSRRFITFVLFPRHFQTKELQFHSICQL
TLFRNYFHYHIKCSKAYMHSRMRFRVDSFIKVLNRAKVDEDDENDELSAEGRQQARRT
FTGRKIVY"
gene complement(<694361..>694822)
/gene="MRPS12"
/locus_tag="YNR036C"
/gene_synonym="uS12m"
/db_xref="GeneID:855772"
mRNA complement(<694361..>694822)
/gene="MRPS12"
/locus_tag="YNR036C"
/gene_synonym="uS12m"
/product="mitochondrial 37S ribosomal protein uS12m
MRPS12"
/transcript_id="NM_001183213.1"
/db_xref="GeneID:855772"
CDS complement(694361..694822)
/gene="MRPS12"
/locus_tag="YNR036C"
/gene_synonym="uS12m"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:30520011]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:28154081]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005763
mitochondrial small ribosomal subunit [PMID:28154081]"
/note="Mitochondrial protein; may interact with ribosomes
based on co-purification experiments; similar to E. coli
and human mitochondrial S12 ribosomal proteins"
/codon_start=1
/product="mitochondrial 37S ribosomal protein uS12m
MRPS12"
/protein_id="NP_014434.1"
/db_xref="GeneID:855772"
/db_xref="SGD:S000005319"
/translation="MLSRFMSNTWCTPLRQAQRLFSSTTTMQATLNQIKRGSGPPRRK
KISTAPQLDQCPQRKGVVLRVMVLKPKKPNSAQRKACRVRLTNGNVVSAYIPGEGHDA
QEHSIVYVRGGRCQDLPGVKYHVIRGAGDLSGVVNRISSRSKYGAKKPSKS"
gene complement(<695052..>695327)
/gene="RSM19"
/locus_tag="YNR037C"
/gene_synonym="uS19m"
/db_xref="GeneID:855773"
mRNA complement(<695052..>695327)
/gene="RSM19"
/locus_tag="YNR037C"
/gene_synonym="uS19m"
/product="mitochondrial 37S ribosomal protein uS19m RSM19"
/transcript_id="NM_001183214.3"
/db_xref="GeneID:855773"
CDS complement(695052..695327)
/gene="RSM19"
/locus_tag="YNR037C"
/gene_synonym="uS19m"
/experiment="EXISTENCE:curator inference:GO:0032543
mitochondrial translation [PMID:11278769]"
/experiment="EXISTENCE:direct assay:GO:0003735 structural
constituent of ribosome [PMID:11278769]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005763
mitochondrial small ribosomal subunit [PMID:11278769]"
/note="Mitochondrial ribosomal protein of the small
subunit; has similarity to E. coli S19 ribosomal protein"
/codon_start=1
/product="mitochondrial 37S ribosomal protein uS19m RSM19"
/protein_id="NP_014435.3"
/db_xref="GeneID:855773"
/db_xref="SGD:S000005320"
/translation="MQPAARLLSRSVWKGPNIVPLPIREAMTKGTPIRTNARAATILP
QFVGLKFQIHNGKEYVPIEISEDMVGHKLGEFAPTRKRFSYTQTKNK"
gene <695595..>697484
/gene="DBP6"
/locus_tag="YNR038W"
/db_xref="GeneID:855774"
mRNA <695595..>697484
/gene="DBP6"
/locus_tag="YNR038W"
/product="putative ATP-dependent RNA helicase DBP6"
/transcript_id="NM_001183215.1"
/db_xref="GeneID:855774"
CDS 695595..697484
/gene="DBP6"
/locus_tag="YNR038W"
/EC_number="3.6.4.13"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:9528757]"
/experiment="EXISTENCE:direct assay:GO:0030687
preribosome, large subunit precursor
[PMID:15126390|PMID:17145778]"
/experiment="EXISTENCE:mutant phenotype:GO:0000463
maturation of LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:9528757]"
/experiment="EXISTENCE:mutant phenotype:GO:0000466
maturation of 5.8S rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:9528757]"
/note="Essential protein involved in ribosome biogenesis;
putative ATP-dependent RNA helicase of the DEAD-box
protein family; human homolog DDX51 complements yeast dbp6
mutant"
/codon_start=1
/product="putative ATP-dependent RNA helicase DBP6"
/protein_id="NP_014436.1"
/db_xref="GeneID:855774"
/db_xref="SGD:S000005321"
/translation="MFASRFDPSQLTAPAASAPEGIVGTTPPAIVPLKRQATESDNEE
YGSHQDSDESSNSSSEEDEDRMQVDYGASEEDSSEVEEEESKPSTHSTVLSRFKQTVS
LQERLGASDIAESKEDEGIEDEAASTHQLKQIPQPEFVKNPMNLNTNSLQFKSTGWLN
TEKIYYDNSLIKPFSDYANELEAKLLQNICKNFSTNTFPIQSIILDSILPVLNFTLNV
SKRNFTRRIGDILVNAATGSGKTLAYSIPIVQTLFKRQINRLRCIIIVPTKLLINQVY
TTLTKLTQGTSLIVSIAKLENSLKDEHKKLSNLEPDILITTPGRLVDHLNMKSINLKN
LKFLIIDEADRLLNQSFQGWCPKLMSHLKTDKLDTLPGNVIKMIFSATLTTNTEKLNG
LNLYKPKLFLKQTDKLYQLPNKLNEFNINIPTAKSVYKPLILLYSICQFMAHSPIAAK
ILIFVKSNESSIRLSKLLQLICESRSQSSVLKNLQNLAVSINSVNSNNSKAENKKIVA
NFSHHSESAGITILITTDIMSRGIDINDITQVINYDPPMSSQQYVHRVGRTARANELG
SAYNLLVGRGERTFFDDLNKDLDRDGKSVQPLELDFTLLESDSELYTSSLESLKNYHN
NTAQA"
gene complement(<697614..>699431)
/gene="ZRG17"
/locus_tag="YNR039C"
/db_xref="GeneID:855775"
mRNA complement(<697614..>699431)
/gene="ZRG17"
/locus_tag="YNR039C"
/product="Zn(2+) transporter ZRG17"
/transcript_id="NM_001183216.1"
/db_xref="GeneID:855775"
CDS complement(697614..699431)
/gene="ZRG17"
/locus_tag="YNR039C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095|PMID:26928762|PMID:15961382]"
/experiment="EXISTENCE:genetic interaction:GO:0005385 zinc
ion transmembrane transporter activity [PMID:15961382]"
/experiment="EXISTENCE:genetic interaction:GO:0006829 zinc
ion transport [PMID:15961382]"
/experiment="EXISTENCE:mutant phenotype:GO:0005385 zinc
ion transmembrane transporter activity [PMID:15961382]"
/experiment="EXISTENCE:mutant phenotype:GO:0006829 zinc
ion transport [PMID:15961382|PMID:10978274]"
/note="Endoplasmic reticulum zinc transporter; part of a
heterodimeric transporter with Msc2p that transfers zinc
from the cytosol to the ER lumen; member of the cation
diffusion facilitator family of efflux pumps;
zinc-regulated directly through Zap1p; transcription
induced under conditions of zinc deficiency"
/codon_start=1
/product="Zn(2+) transporter ZRG17"
/protein_id="NP_014437.1"
/db_xref="GeneID:855775"
/db_xref="SGD:S000005322"
/translation="METPQMNAIQEEDNLSPEVAFQTPKLNDSDASSFSLSNMNAVGN
VDGIPSQNRTFFASPRPSSLFYSAKEGNNSSSSIIYNPSFTFGENASSNANINEAALM
KGKGNEGRRQSLKYIPAPKLVPPPPRTRSPVRGISPDAGSSKRSSMTLDSPFNFTTST
LQPHQQTPPSSAASRTSFRKGHRYKHSSVSMNFFQEPEVKIPLNIAKSLPIPDFNDLL
SNLPWPKAYIQLSIAALQIFACLITFQVGHLYSWSNFITLSHFITYDIIGSLVIIFVE
NLSQFQVWFTGTITFPFGLNRIDVLLSFALAVSLCFVGLDLLFHIIEEFIVLFVESGS
SLTNNHDHDEINEQIPHSHIANANDSQNENITLWYSILMINLVLSTLSLYKTFYANKY
SNLKTKNPIITITYTAYLFIYPLLLDLLSSISDYLATLVISSLILWHGLTIARWTSTV
LLMGFSTTSLSNSALFNNNDSTDTTAHTQQVESKAAKEKPSVRPRSMSSLPIATKNTK
IRKTGFLNSAGFTENPTTIKNMIKDQIERLSEFKSRYILNYDDIVISKVNFTLYVVLI
KITMKGGSDDDELMLRLAIDKCIQTSIPTCETTIDIDRI"
gene <699690..>700460
/gene="MRX15"
/locus_tag="YNR040W"
/gene_synonym="DPI29"
/db_xref="GeneID:855776"
mRNA <699690..>700460
/gene="MRX15"
/locus_tag="YNR040W"
/gene_synonym="DPI29"
/product="Mrx15p"
/transcript_id="NM_001183217.3"
/db_xref="GeneID:855776"
CDS 699690..700460
/gene="MRX15"
/locus_tag="YNR040W"
/gene_synonym="DPI29"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:24769239|PMID:16823961|PMID:14562095|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:30091672]"
/experiment="EXISTENCE:direct assay:GO:0097177
mitochondrial ribosome binding [PMID:30091672]"
/experiment="EXISTENCE:genetic interaction:GO:0032979
protein insertion into mitochondrial inner membrane from
matrix [PMID:30091672]"
/experiment="EXISTENCE:mutant phenotype:GO:0097177
mitochondrial ribosome binding [PMID:30091672]"
/note="Membrane-associated mitoribosome receptor; involved
along with Mba1p in the cotranslational insertion of
nascent polypeptide chains into the inner mitochondrial
membrane; associates with the mitochondrial large
ribosomal subunit and nascent polypeptide chains; null
mutant displays increased tolerance to
mistranslation-induced proteotoxic stress; localizes to
the inner mitochondrial membrane"
/codon_start=1
/product="Mrx15p"
/protein_id="NP_014438.3"
/db_xref="GeneID:855776"
/db_xref="SGD:S000005323"
/translation="MTNMAAKNQFKGSSFTLAQLIEEVGRNGGKKPVFQYKVPRSIRW
ASTALAVVFLTYGAAYTDMSWRTAREVYGNATEEEKHSPWFKCKTFGPVALGVLPVIL
AAATKHVTSRLVTEMKYLPPLKNSTVPRCQLTRRTYLLGRPVSITREINELSKNKATK
IFTGVGSQGMEDKATFVFFTVDEKAPSFFNKFYIFSRSGSVVKNDARILDCFFNSVAE
NKLLNRSILTQILSHTSAKTLFHSGNSRSSIKNIVKPK"
gene complement(<700541..>701659)
/gene="COQ2"
/locus_tag="YNR041C"
/db_xref="GeneID:855778"
mRNA complement(<700541..>701659)
/gene="COQ2"
/locus_tag="YNR041C"
/product="4-hydroxybenzoate octaprenyltransferase"
/transcript_id="NM_001183218.3"
/db_xref="GeneID:855778"
CDS complement(700541..701659)
/gene="COQ2"
/locus_tag="YNR041C"
/EC_number="2.5.1.39"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:1740455|PMID:10469659|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0031966
mitochondrial membrane [PMID:359053]"
/experiment="EXISTENCE:genetic interaction:GO:0006744
ubiquinone biosynthetic process [PMID:15548532]"
/experiment="EXISTENCE:genetic interaction:GO:0008412
4-hydroxybenzoate polyprenyltransferase activity
[PMID:7765507]"
/experiment="EXISTENCE:mutant phenotype:GO:0006744
ubiquinone biosynthetic process [PMID:1740455]"
/experiment="EXISTENCE:mutant phenotype:GO:0008412
4-hydroxybenzoate polyprenyltransferase activity
[PMID:1740455]"
/note="Para hydroxybenzoate polyprenyl transferase;
catalyzes the second step in ubiquinone (coenzyme Q)
biosynthesis; human COQ2, mutations in which are
implicated in an increased risk of mutiple-system atrophy,
can complement a yeast coq2 null mutant"
/codon_start=1
/product="4-hydroxybenzoate octaprenyltransferase"
/protein_id="NP_014439.3"
/db_xref="GeneID:855778"
/db_xref="SGD:S000005324"
/translation="MFIWQRKSILLGRSILGSGRVTVAGIIGSSRKRYTSSSSSSSSP
SSKESAPVFTSKELEVARKERLDGLGPFVSRLPKKWIPYAELMRLEKPVGTWLLYLPC
SWSILMGAMMQGATLSATAGMLGIFGVGALVMRGAGCTINDFLDRKLDQRVIRSVERP
IASGRVSPRRALVFLGAQTLVGMGVLSLLPAQCWWLGLASLPIVFTYPLFKRFTYYPQ
AALSACFNWGALLGFPAMGVMSWPTMIPLYLSSYLWCMTYDTIYAHQDKKFDIKAGIK
STALAWGPRTKSIMKAMSASQIALLAVAGLNSGLLWGPGFIGGLGVFAYRLFSMIKKV
DLDNPKNCWKYFNANINTGLYFTYALAVDYILRLFGFL"
gene <701895..>703085
/gene="MVD1"
/locus_tag="YNR043W"
/gene_synonym="ERG19"
/db_xref="GeneID:855779"
mRNA <701895..>703085
/gene="MVD1"
/locus_tag="YNR043W"
/gene_synonym="ERG19"
/product="diphosphomevalonate decarboxylase MVD1"
/transcript_id="NM_001183220.1"
/db_xref="GeneID:855779"
CDS 701895..703085
/gene="MVD1"
/locus_tag="YNR043W"
/gene_synonym="ERG19"
/EC_number="4.1.1.33"
/experiment="EXISTENCE:direct assay:GO:0004163
diphosphomevalonate decarboxylase activity
[PMID:8626466|PMID:15169949]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006696 ergosterol
biosynthetic process [PMID:8626466]"
/experiment="EXISTENCE:mutant phenotype:GO:0004163
diphosphomevalonate decarboxylase activity
[PMID:15169949|PMID:1779710]"
/experiment="EXISTENCE:mutant phenotype:GO:0016126 sterol
biosynthetic process [PMID:9244250]"
/note="Mevalonate pyrophosphate decarboxylase; essential
enzyme involved in the biosynthesis of isoprenoids and
sterols, including ergosterol; acts as a homodimer"
/codon_start=1
/product="diphosphomevalonate decarboxylase MVD1"
/protein_id="NP_014441.1"
/db_xref="GeneID:855779"
/db_xref="SGD:S000005326"
/translation="MTVYTASVTAPVNIATLKYWGKRDTKLNLPTNSSISVTLSQDDL
RTLTSAATAPEFERDTLWLNGEPHSIDNERTQNCLRDLRQLRKEMESKDASLPTLSQW
KLHIVSENNFPTAAGLASSAAGFAALVSAIAKLYQLPQSTSEISRIARKGSGSACRSL
FGGYVAWEMGKAEDGHDSMAVQIADSSDWPQMKACVLVVSDIKKDVSSTQGMQLTVAT
SELFKERIEHVVPKRFEVMRKAIVEKDFATFAKETMMDSNSFHATCLDSFPPIFYMND
TSKRIISWCHTINQFYGETIVAYTFDAGPNAVLYYLAENESKLFAFIYKLFGSVPGWD
KKFTTEQLEAFNHQFESSNFTARELDLELQKDVARVILTQVGSGPQETNESLIDAKTG
LPKE"
gene <703699..>705876
/gene="AGA1"
/locus_tag="YNR044W"
/db_xref="GeneID:855780"
mRNA <703699..>705876
/gene="AGA1"
/locus_tag="YNR044W"
/product="Aga1p"
/transcript_id="NM_001183221.1"
/db_xref="GeneID:855780"
CDS 703699..705876
/gene="AGA1"
/locus_tag="YNR044W"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:2072914|PMID:7957044]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0000752
agglutination involved in conjugation with cellular fusion
[PMID:2072914|PMID:7957044]"
/experiment="EXISTENCE:mutant phenotype:GO:0050839 cell
adhesion molecule binding [PMID:2072914|PMID:7957044]"
/note="Anchorage subunit of a-agglutinin of a-cells;
highly O-glycosylated protein with N-terminal secretion
signal and C-terminal signal for addition of GPI anchor to
cell wall, linked to adhesion subunit Aga2p via two
disulfide bonds; AGA1 has a paralog, FIG2, that arose from
the whole genome duplication"
/codon_start=1
/product="Aga1p"
/protein_id="NP_014442.1"
/db_xref="GeneID:855780"
/db_xref="SGD:S000005327"
/translation="MTLSFAHFTYLFTILLGLTNIALASDPETILVTITKTNDANGVV
TTTVSPALVSTSTIVQAGTTTLYTTWCPLTVSTSSAAEISPSISYATTLSRFSTLTLS
TEVCSHEACPSSSTLPTTTLSVTSKFTSYICPTCHTTAISSLSEVGTTTVVSSSAIEP
SSASIISPVTSTLSSTTSSNPTTTSLSSTSTSPSSTSTSPSSTSTSSSSTSTSSSSTS
TSSSSTSTSPSSTSTSSSLTSTSSSSTSTSQSSTSTSSSSTSTSPSSTSTSSSSTSTS
PSSKSTSASSTSTSSYSTSTSPSLTSSSPTLASTSPSSTSISSTFTDSTSSLGSSIAS
SSTSVSLYSPSTPVYSVPSTSSNVATPSMTSSTVETTVSSQSSSEYITKSSISTTIPS
FSMSTYFTTVSGVTTMYTTWCPYSSESETSTLTSMHETVTTDATVCTHESCMPSQTTS
LITSSIKMSTKNVATSVSTSTVESSYACSTCAETSHSYSSVQTASSSSVTQQTTSTKS
WVSSMTTSDEDFNKHATGKYHVTSSGTSTISTSVSEATSTSSIDSESQEQSSHLLSTS
VLSSSSLSATLSSDSTILLFSSVSSLSVEQSPVTTLQISSTSEILQPTSSTAIATISA
STSSLSATSISTPSTSVESTIESSSLTPTVSSIFLSSSSAPSSLQTSVTTTEVSTTSI
SIQYQTSSMVTISQYMGSGSQTRLPLGKLVFAIMAVACNVIFS"
gene <706139..>707608
/gene="PET494"
/locus_tag="YNR045W"
/db_xref="GeneID:855781"
mRNA <706139..>707608
/gene="PET494"
/locus_tag="YNR045W"
/product="Pet494p"
/transcript_id="NM_001183222.3"
/db_xref="GeneID:855781"
CDS 706139..707608
/gene="PET494"
/locus_tag="YNR045W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005743
mitochondrial inner membrane [PMID:8389363]"
/experiment="EXISTENCE:mutant phenotype:GO:0045182
translation regulator activity [PMID:2833749]"
/experiment="EXISTENCE:mutant phenotype:GO:0070131
positive regulation of mitochondrial translation
[PMID:2833749]"
/note="Mitochondrial translational activator specific for
the COX3 mRNA; acts together with Pet54p and Pet122p;
located in the mitochondrial inner membrane"
/codon_start=1
/product="Pet494p"
/protein_id="NP_014443.3"
/db_xref="GeneID:855781"
/db_xref="SGD:S000005328"
/translation="MHLKKGKRSISTVWRLLWKRFYSVNSKTNMHFSRSRKKPVTNFT
RTNGLLLSCNGDTFPYLRTLWRYFNAPGNLMFVTTNIVAFMGIVTYNTLVTISSERAF
EEQMMAAQVSLAKQREELETTALSLPRDIELRGEEDDIKWEQPDVAHVREDPLVEEQN
AKLDTPIKQYTLGDLILNKRENVTDYDSQRAKASIFHMLYAYMLYRDVIQPTTMTQNN
NSEEWRREVELLTKGKEVQGTHRRIDVFYDLWNKNFDKIVTSPEKVQNFQLPNWSKYP
TILKFICTELHDNSLKTLGEFKQFYGKVRSNEVKKLLGLWLYDHSFLFPHNIYDNRTE
EDFYDILINDSMQDNRIFQKYSSIVMNPYNERTQLFFPNVNSPSVNKPVPSISLETYT
RLLKGYINLQETGCKYDYNDNIFKLISILKLNCFLQRNKKKHAGPTVRILLPRDEDRS
QILGTISQAEKRTCYQILSKNRDVVALLKRISDIQADSS"
gene <707788..>708195
/gene="TRM112"
/locus_tag="YNR046W"
/db_xref="GeneID:855782"
mRNA <707788..>708195
/gene="TRM112"
/locus_tag="YNR046W"
/product="RNA methylation protein TRM112"
/transcript_id="NM_001183223.1"
/db_xref="GeneID:855782"
CDS 707788..708195
/gene="TRM112"
/locus_tag="YNR046W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922|PMID:14690591|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:14690591]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14690591|PMID:14562095|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0008276 protein
methyltransferase activity [PMID:17008308]"
/experiment="EXISTENCE:direct assay:GO:0018364
peptidyl-glutamine methylation [PMID:17008308]"
/experiment="EXISTENCE:direct assay:GO:0160102 tRNA
(guanine(10)-N2)-methyltransferase activity
[PMID:15899842]"
/experiment="EXISTENCE:mutant phenotype:GO:0000470
maturation of LSU-rRNA [PMID:22956767]"
/experiment="EXISTENCE:mutant phenotype:GO:0016435 rRNA
(guanine) methyltransferase activity [PMID:22493060]"
/experiment="EXISTENCE:mutant phenotype:GO:0030488 tRNA
methylation [PMID:15899842]"
/experiment="EXISTENCE:mutant phenotype:GO:0030490
maturation of SSU-rRNA [PMID:22956767]"
/experiment="EXISTENCE:mutant phenotype:GO:0042273
ribosomal large subunit biogenesis [PMID:22956767]"
/experiment="EXISTENCE:mutant phenotype:GO:0042274
ribosomal small subunit biogenesis [PMID:22956767]"
/experiment="EXISTENCE:mutant phenotype:GO:0070476 rRNA
(guanine-N7)-methylation [PMID:22493060]"
/experiment="EXISTENCE:mutant phenotype:GO:0160102 tRNA
(guanine(10)-N2)-methyltransferase activity
[PMID:15899842]"
/experiment="EXISTENCE:physical interaction:GO:0035657
eRF1 methyltransferase complex [PMID:17008308]"
/experiment="EXISTENCE:physical interaction:GO:0043528
tRNA (m2G10) methyltransferase complex [PMID:15899842]"
/note="Protein involved in methylation of tRNA, rRNA, and
translation factors; also involved in ribosome biogenesis;
subunit of tRNA methyltransferase (MTase) complexes in
combination with Trm9p and Trm11p; N7-methylates G1575 of
18S rRNA as complex with Bud23p; subunit of complex with
Mtq2p that methylates Sup45p (eRF1) in the ternary complex
eRF1-eRF3-GTP; relative distribution to the nucleus
increases upon DNA replication stress; functional homolog
of human TRMT112"
/codon_start=1
/product="RNA methylation protein TRM112"
/protein_id="NP_014444.1"
/db_xref="GeneID:855782"
/db_xref="SGD:S000005329"
/translation="MKFLTTNFLKCSVKACDTSNDNFPLQYDGSKCQLVQDESIEFNP
EFLLNIVDRVDWPAVLTVAAELGNNALPPTKPSFPSSIQELTDDDMAILNDLHTLLLQ
TSIAEGEMKCRNCGHIYYIKNGIPNLLLPPHLV"
gene <708523..>711204
/gene="FPK1"
/locus_tag="YNR047W"
/db_xref="GeneID:855783"
mRNA <708523..>711204
/gene="FPK1"
/locus_tag="YNR047W"
/product="serine/threonine protein kinase FPK1"
/transcript_id="NM_001183224.1"
/db_xref="GeneID:855783"
CDS 708523..711204
/gene="FPK1"
/locus_tag="YNR047W"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:18199685]"
/experiment="EXISTENCE:direct assay:GO:0004674 protein
serine/threonine kinase activity
[PMID:19966303|PMID:29895620|PMID:28069741]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:18199685|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:19966303]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:25378585]"
/experiment="EXISTENCE:genetic interaction:GO:0001932
regulation of protein phosphorylation [PMID:28069741]"
/experiment="EXISTENCE:genetic interaction:GO:0004674
protein serine/threonine kinase activity [PMID:19966303]"
/experiment="EXISTENCE:genetic interaction:GO:0061092
positive regulation of phospholipid translocation
[PMID:18199685]"
/experiment="EXISTENCE:mutant phenotype:GO:0000749
response to pheromone triggering conjugation with cellular
fusion [PMID:11337509]"
/experiment="EXISTENCE:mutant phenotype:GO:0004674 protein
serine/threonine kinase activity [PMID:19966303]"
/experiment="EXISTENCE:mutant phenotype:GO:2000370
positive regulation of clathrin-dependent endocytosis
[PMID:28069741]"
/note="Ser/Thr protein kinase; phosphorylates several
aminophospholipid translocase family members, regulating
phospholipid translocation and membrane asymmetry;
phosphorylates and inhibits the protein kinase Akl1p,
stimulating endocytosis; phosphorylates and inhibits
upstream inhibitory kinase Ypk1p; localizes to the
cytoplasm, early endosome/TGN, the plasma membrane and the
shmoo tip; redundant role with KIN82 in the mating
pheromone response; activity stimulated by Gin4p kinase
and sphingolipid MIPC"
/codon_start=1
/product="serine/threonine protein kinase FPK1"
/protein_id="NP_014445.1"
/db_xref="GeneID:855783"
/db_xref="SGD:S000005330"
/translation="MAGHHHEHEQERDHEQEHEHDSLQRPTTGSERTRSISFSKLLTR
SWKRNASSSNNMSVSSVNLYSDPENSRESDHNNSGSEGQSSRFSKLKSMFQSGNSSKN
ASAHNSSQSSLEGDSASSSSKLRYVKPMTSVANASPASPPLSPTIPETDVLQTPKMVH
IDQHEHEREHSNCGSPIMLSSSSFSPTVARTGTGRRRSPSTPIMPSQNSNNSSSTSAI
RPNNYRHHSGSQGFSSNNPFRERAGTVRSSNPYFAYQGLPTHAMSSHDLDEGFQPYAN
GSGIHFLSTPTSKTNSLTNTKNLSNLSLNEIKENEEVQEFNNEDFFFHDIPKDLSLKD
TLNGSPSRGSSKSPTITQTFPSIIVGFDNEYEEDNNNDKHDEKEEQQTTTDNKTRNLS
PTKQNGKATHPRIKIPLRRAASEPNGLQLASATSPTSSSARKTSGSSNINDKIPGQSV
PPPNSFFPQEPSPKISDFPEPRRSRRLRTKSFSNKFQDIMVGPQSFEKIRLLGQGDVG
KVFLVREKKTNRVYALKVLSKDEMIKRNKIKRVLTEQEILATSNHPFIVTLYHSFQSE
DYLYLCMEYCMGGEFFRALQTRKTKCICEDDARFYASEVTAALEYLHLLGFIYRDLKP
ENILLHQSGHIMLSDFDLSIQAKDSKVPVVKGSAQSTLVDTKICSDGFRTNSFVGTEE
YIAPEVIRGNGHTAAVDWWTLGILIYEMLFGFTPFKGDNTNETFTNILKNEVSFPNNN
EISRTCKDLIKKLLTKNESKRLGCKMGAADVKKHPFFKKVQWSLLRNQEPPLIPVLSE
DGYDFAKLSSNKKRQTSQDSHKHLDEQEKNMFEERVEYDDEVSEDDPFHDFNSMSLME
QDNNSMIYGNTNSYGKIAYTPNSNRSRSNSHRTFFKR"
gene <711630..>712811
/locus_tag="YNR048W"
/gene_synonym="CRF1"
/db_xref="GeneID:855784"
mRNA <711630..>712811
/locus_tag="YNR048W"
/gene_synonym="CRF1"
/product="putative aminophospholipid translocase
regulatory protein"
/transcript_id="NM_001183225.3"
/db_xref="GeneID:855784"
CDS 711630..712811
/locus_tag="YNR048W"
/gene_synonym="CRF1"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0045332
phospholipid translocation [PMID:12842877]"
/experiment="EXISTENCE:physical interaction:GO:0005802
trans-Golgi network [PMID:17093059]"
/note="Potential noncatalytic subunit for phospholipid
translocase Dnf3p; YNR048W has a paralog, CDC50, that
arose from the whole genome duplication"
/codon_start=1
/product="putative aminophospholipid translocase
regulatory protein"
/protein_id="NP_014446.3"
/db_xref="GeneID:855784"
/db_xref="SGD:S000005331"
/translation="MGLILRWKEKKQLSSKQNAQKSRKPANTSFRQQRLKAWQPILSP
QSVLPLLILMACVFAPIGIGLVVSTISVQRLVVNYTECDALAPAKHFETIPSEYVDYH
FSKKVAVQPQWMVLTDPELGNQTCRIQFEVPNHIKKSTYVYYRLTNFNQNYREYVQSL
DLDQLKGKALIGNDLDPNCDPLRTVENKTIFPCGLIANSMFNDTFGTTLTGVNDTADY
LLTTKGIAWDTDSHRYGKTEYNASDIVPPPNWAKLFPNGYTDDNIPDLQNWEQFKIWM
RTAALPNFYKLAMKNETNGLGKGIYIADIELNYPVRSFYGTKSFVLTTNSIIGAGNEA
LGIVYLIVAGIATLFAILFLIKVIFKPRPMHDHSYLNFENSDTPFDESSVVSIPLREI
L"
gene complement(<713023..>713655)
/gene="MSO1"
/locus_tag="YNR049C"
/db_xref="GeneID:855785"
mRNA complement(<713023..>713655)
/gene="MSO1"
/locus_tag="YNR049C"
/product="Mso1p"
/transcript_id="NM_001183226.3"
/db_xref="GeneID:855785"
CDS complement(713023..713655)
/gene="MSO1"
/locus_tag="YNR049C"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:16030256|PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16030256|PMID:16087665]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:16030256|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:26928762|PMID:16030256|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0006904 vesicle
docking involved in exocytosis [PMID:9207091]"
/experiment="EXISTENCE:direct assay:GO:0031201 SNARE
complex [PMID:16087665]"
/experiment="EXISTENCE:direct assay:GO:0033101 cellular
bud membrane [PMID:16087665]"
/experiment="EXISTENCE:mutant phenotype:GO:0006904 vesicle
docking involved in exocytosis [PMID:9207091]"
/experiment="EXISTENCE:mutant phenotype:GO:0032120
ascospore-type prospore membrane formation
[PMID:16030256]"
/experiment="EXISTENCE:mutant phenotype:GO:0061025
membrane fusion [PMID:16030256]"
/note="Lipid-interacting protein in SNARE complex assembly
machinery; acts at late step in secretion; interacts with
membranes through two distinct binding sites; shows
genetic and physical interactions with Sec1p; required for
prospore membrane formation during sporulation; N-terminus
closely associates with plasma membrane, C-terminus
colocalizes with Sec4p on intracellular membranes;
relocalizes from bud neck to nucleus upon DNA replication
stress"
/codon_start=1
/product="Mso1p"
/protein_id="NP_014447.3"
/db_xref="GeneID:855785"
/db_xref="SGD:S000005332"
/translation="MMSQVSHSQEGSGRFWNKFKSSTKSLSTSLAHLSIKAEKDGDTV
NTTLVHKGLVKFYENQHPFQGFPGWLGEKEDLPNERKILDTQVKHDMKKQNSRHFSPS
FSNRRKASSEDPMGTPSSNGNTPEYTPASKSFQDIYNNHTSSSSATPRRASSRPTRPS
AGQEFRASLGRSKTSNSFNTSSTPTPPPDASSGVMAMKDRLKRRNNDYGF"
gene complement(<714048..>715388)
/gene="LYS9"
/locus_tag="YNR050C"
/gene_synonym="LYS13"
/db_xref="GeneID:855786"
mRNA complement(<714048..>715388)
/gene="LYS9"
/locus_tag="YNR050C"
/gene_synonym="LYS13"
/product="saccharopine dehydrogenase (NADP+,
L-glutamate-forming)"
/transcript_id="NM_001183227.1"
/db_xref="GeneID:855786"
CDS complement(714048..715388)
/gene="LYS9"
/locus_tag="YNR050C"
/gene_synonym="LYS13"
/EC_number="1.5.1.10"
/experiment="EXISTENCE:direct assay:GO:0004755
saccharopine dehydrogenase (NADP+, L-glutamate-forming)
activity [PMID:18416559]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0019878
L-lysine biosynthetic process via aminoadipic acid
[PMID:6429126]"
/note="Saccharopine dehydrogenase (NADP+,
L-glutamate-forming); catalyzes the formation of
saccharopine from alpha-aminoadipate 6-semialdehyde, the
seventh step in lysine biosynthesis pathway; exhibits
genetic and physical interactions with TRM112"
/codon_start=1
/product="saccharopine dehydrogenase (NADP+,
L-glutamate-forming)"
/protein_id="NP_014448.1"
/db_xref="GeneID:855786"
/db_xref="SGD:S000005333"
/translation="MGKNVLLLGSGFVAQPVIDTLAANDDINVTVACRTLANAQALAK
PSGSKAISLDVTDDSALDKVLADNDVVISLIPYTFHPNVVKSAIRTKTDVVTSSYISP
ALRELEPEIVKAGITVMNEIGLDPGIDHLYAVKTIDEVHRAGGKLKSFLSYCGGLPAP
EDSDNPLGYKFSWSSRGVLLALRNSAKYWKDGKIETVSSEDLMATAKPYFIYPGYAFV
CYPNRDSTLFKDLYHIPEAETVIRGTLRYQGFPEFVKALVDMGMLKDDANEIFSKPIA
WNEALKQYLGAKSTSKEDLIASIDSKATWKDDEDRERILSGFAWLGLFSDAKITPRGN
ALDTLCARLEELMQYEDNERDMVVLQHKFGIEWADGTTETRTSTLVDYGKVGGYSSMA
ATVGYPVAIATKFVLDGTIKGPGLLAPYSPEINDPIMKELKDKYGIYLKEKTVA"
gene 716120..716284
/gene="SNR49"
/locus_tag="YNCN0018W"
/db_xref="GeneID:9164966"
ncRNA 716120..716284
/ncRNA_class="snoRNA"
/gene="SNR49"
/locus_tag="YNCN0018W"
/product="SNR49"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:15306656]"
/experiment="EXISTENCE:mutant phenotype:GO:0000454 snoRNA
guided rRNA pseudouridine synthesis
[PMID:15306656|PMID:15923376]"
/experiment="EXISTENCE:mutant phenotype:GO:0030559 rRNA
pseudouridylation guide activity
[PMID:15923376|PMID:15306656]"
/experiment="EXISTENCE:physical interaction:GO:0031429 box
H/ACA snoRNP complex [PMID:15923376]"
/note="H/ACA box small nucleolar RNA (snoRNA); guides
pseudouridylation of large subunit (LSU) rRNA at position
U990 and small subunit (SSU) rRNA at positions U120, U211,
and U302"
/transcript_id="NR_132253.1"
/db_xref="GeneID:9164966"
/db_xref="SGD:S000007460"
gene complement(<716780..>718327)
/gene="BRE5"
/locus_tag="YNR051C"
/db_xref="GeneID:855787"
mRNA complement(<716780..>718327)
/gene="BRE5"
/locus_tag="YNR051C"
/product="Bre5p"
/transcript_id="NM_001183228.1"
/db_xref="GeneID:855787"
CDS complement(716780..718327)
/gene="BRE5"
/locus_tag="YNR051C"
/experiment="EXISTENCE:direct assay:GO:0000932 P-body
[PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0045053
protein retention in Golgi apparatus [PMID:32673164]"
/experiment="EXISTENCE:mutant phenotype:GO:0016579 protein
deubiquitination [PMID:12778054]"
/experiment="EXISTENCE:mutant phenotype:GO:0034517
ribophagy [PMID:18391941]"
/experiment="EXISTENCE:mutant phenotype:GO:0045053 protein
retention in Golgi apparatus [PMID:32673164]"
/experiment="EXISTENCE:mutant phenotype:GO:0060628
regulation of ER to Golgi vesicle-mediated transport
[PMID:12778054]"
/experiment="EXISTENCE:mutant phenotype:GO:2000156
regulation of retrograde vesicle-mediated transport, Golgi
to ER [PMID:14593109]"
/experiment="EXISTENCE:physical interaction:GO:1990861
Ubp3-Bre5 deubiquitination complex [PMID:12778054]"
/note="Ubiquitin protease cofactor; forms deubiquitination
complex with Ubp3p that coregulates anterograde and
retrograde transport between the ER and Golgi
compartments, deubiquitinating COPII and COPI vesicle coat
constituents, Sec23p and Sec27p; involved along with Ubp3p
in the steady-state retention of Golgi membrane proteins,
such as glycosyltransferases; null is sensitive to
brefeldin A"
/codon_start=1
/product="Bre5p"
/protein_id="NP_014449.1"
/db_xref="GeneID:855787"
/db_xref="SGD:S000005334"
/translation="MGVTVQDICFAFLQNYYERMRTDPSKLAYFYASTAELTHTNYQS
KSTNEKDDVLPTVKVTGRENINKFFSRNDAKVRSLKLKLDTIDFQYTGHLHKSILIMA
TGEMFWTGTPVYKFCQTFILLPSSNGSTFDITNDIIRFISNSFKPYVLTDASLSQSNE
ENSVSAVEEDKIRHESGVEKEKEKEKSPEISKPKAKKETVKDTTAPTESSTQEKPIVD
HSQPRAIPVTKESKIHTETVPSSTKGNHKQDEVSTEELGNVTKLNEKSHKAEKKAAPI
KTKEGSVEAINAVNNSSLPNGKEVSDEKPVPGGVKEAETEIKPIEPQVSDAKESGNNA
STPSSSPEPVANPPKMTWASKLMNENSDRISKNNTTVEYIRPETLPKKPTERKFEMGN
RRDNASANSKNKKKPVFSTVNKDGFYPIYIRGTNGLREEKLRSALEKEFGKVMRITAA
DNFAVVDFETQKSQIDALEKKKKSIDGIEVCLERKTVKKPTSNNPPGIFTNGTRSHRK
QPLKRKD"
gene complement(<719346..>720647)
/gene="POP2"
/locus_tag="YNR052C"
/gene_synonym="CAF1"
/db_xref="GeneID:855788"
mRNA complement(<719346..>720647)
/gene="POP2"
/locus_tag="YNR052C"
/gene_synonym="CAF1"
/product="CCR4-NOT core DEDD family RNase subunit POP2"
/transcript_id="NM_001183229.3"
/db_xref="GeneID:855788"
CDS complement(719346..720647)
/gene="POP2"
/locus_tag="YNR052C"
/gene_synonym="CAF1"
/EC_number="3.1.13.4"
/experiment="EXISTENCE:direct assay:GO:0000175 3'-5'-RNA
exonuclease activity [PMID:11410650]"
/experiment="EXISTENCE:direct assay:GO:0000289
nuclear-transcribed mRNA poly(A) tail shortening
[PMID:11410650|PMID:11889048]"
/experiment="EXISTENCE:direct assay:GO:0000932 P-body
[PMID:18611963]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11889048]"
/experiment="EXISTENCE:direct assay:GO:0030015 CCR4-NOT
core complex [PMID:10490603]"
/experiment="EXISTENCE:direct assay:GO:0032968 positive
regulation of transcription elongation by RNA polymerase
II [PMID:21406554]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:19053807]"
/experiment="EXISTENCE:mutant phenotype:GO:0000289
nuclear-transcribed mRNA poly(A) tail shortening
[PMID:11410650]"
/experiment="EXISTENCE:mutant phenotype:GO:0006368
transcription elongation by RNA polymerase II
[PMID:11404327]"
/note="Subunit of Ccr4-Not complex that mediates 3' to 5'
mRNA deadenylation; exonuclease of the DEDD superfamily"
/codon_start=1
/product="CCR4-NOT core DEDD family RNase subunit POP2"
/protein_id="NP_014450.3"
/db_xref="GeneID:855788"
/db_xref="SGD:S000005335"
/translation="MQSMNVQPRVLAVGGEQFFSQRQASEQHQQQNMGPQVYSPKVNR
ARMFPQGMPVNTINGSVNQEMNNAYLLKQKNEPLLTQQQQQQQQQQQPFNIGTPVSVA
SLPPGLNVLQQQQQQQQQQQQQQQGVGLNRPLASQLPKHLTNQSMPPIFLPPPNYLFV
RDVWKSNLYSEFAVIRQLVSQYNHVSISTEFVGTLARPIGTFRSKVDYHYQTMRANVD
FLNPIQLGLSLSDANGNKPDNGPSTWQFNFEFDPKKEIMSTESLELLRKSGINFEKHE
NLGIDVFEFSQLLMDSGLMMDDSVTWITYHAAYDLGFLINILMNDSMPNNKEDFEWWV
HQYMPNFYDLNLVYKIIQEFKNPQLQQSSQQQQQQQYSLTTLADELGLPRFSIFTTTG
GQSLLMLLSFCQLSKLSMHKFPNGTDFAKYQGVIYGIDGDQ"
gene complement(<721120..>723112)
/gene="NOG2"
/locus_tag="YNR053C"
/gene_synonym="NUG2"
/db_xref="GeneID:855789"
mRNA complement(join(<721120..721770,722303..>723112))
/gene="NOG2"
/locus_tag="YNR053C"
/gene_synonym="NUG2"
/product="putative GTPase NOG2"
/transcript_id="NM_001183230.1"
/db_xref="GeneID:855789"
CDS complement(join(721120..721770,722303..723112))
/gene="NOG2"
/locus_tag="YNR053C"
/gene_synonym="NUG2"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:26928762|PMID:10684247]"
/experiment="EXISTENCE:direct assay:GO:0005654 nucleoplasm
[PMID:11707418]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:11707418]"
/experiment="EXISTENCE:direct assay:GO:0030687
preribosome, large subunit precursor
[PMID:23212245|PMID:17443350|PMID:22735702]"
/experiment="EXISTENCE:direct assay:GO:0070180 large
ribosomal subunit rRNA binding [PMID:24240281]"
/experiment="EXISTENCE:mutant phenotype:GO:0000055
ribosomal large subunit export from nucleus
[PMID:11583615|PMID:11707418]"
/experiment="EXISTENCE:mutant phenotype:GO:2000200
regulation of ribosomal subunit export from nucleus
[PMID:24240281]"
/note="Putative GTPase; associates with pre-60S ribosomal
subunits in the nucleolus and is required for their
nuclear export and maturation; recruited by ribosomal
proteins L17, L35, and L37 to assembling ribosomes after
27SB pre-rRNA is generated, immediately preceding removal
of ITS2"
/codon_start=1
/product="putative GTPase NOG2"
/protein_id="NP_014451.1"
/db_xref="GeneID:855789"
/db_xref="SGD:S000005336"
/translation="MGTGKKEKSRRIREGDTKDGNLRVKGENFYRDSKRVKFLNMYTS
GKEIRNKKGNLIRAASFQDSTIPDARVQPDRRWFGNTRVISQDALQHFRSALGETQKD
TYQVLLRRNKLPMSLLEEKDADESPKARILDTESYADAFGPKAQRKRPRLAASNLEDL
VKATNEDITKYEEKQVLDATLGLMGNQEDKENGWTSAAKEAIFSKGQSKRIWNELYKV
IDSSDVVIHVLDARDPLGTRCKSVEEYMKKETPHKHLIYVLNKCDLVPTWVAAAWVKH
LSKERPTLAFHASITNSFGKGSLIQLLRQFSQLHTDRKQISVGFIGYPNTGKSSIINT
LRKKKVCQVAPIPGETKVWQYITLMKRIFLIDCPGIVPPSSKDSEEDILFRGVVRVEH
VTHPEQYIPGVLKRCQVKHLERTYEISGWKDATEFIEILARKQGRLLKGGEPDESGVS
KQILNDFNRGKIPWFVLPPEKEGEEKPKKKEVEKTA"
gene complement(721938..722211)
/gene="SNR191"
/locus_tag="YNCN0019C"
/db_xref="GeneID:9164970"
ncRNA complement(721938..722211)
/ncRNA_class="snoRNA"
/gene="SNR191"
/locus_tag="YNCN0019C"
/product="SNR191"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:12810910]"
/experiment="EXISTENCE:direct assay:GO:0005546
phosphatidylinositol-4,5-bisphosphate binding
[PMID:40863722]"
/experiment="EXISTENCE:mutant phenotype:GO:0030559 rRNA
pseudouridylation guide activity [PMID:12810910]"
/experiment="EXISTENCE:mutant phenotype:GO:0031118 rRNA
pseudouridine synthesis [PMID:12810910]"
/experiment="EXISTENCE:physical interaction:GO:0031429 box
H/ACA snoRNP complex [PMID:12810910|PMID:15923376]"
/note="H/ACA box small nucleolar RNA (snoRNA); guides
pseudouridylation of large subunit (LSU) rRNA at positions
U2258 and U2260; binds to
phosphatidylinositol-4,5-bisphosphate in vitro, and
molecular docking predicts binding within the stem-loop
motif; encoded within the intron of NOG2/YNR053C; sequence
similarity with human LINC01139/LINK-A"
/transcript_id="NR_132254.1"
/db_xref="GeneID:9164970"
/db_xref="SGD:S000029463"
gene complement(<723356..>724306)
/gene="ESF2"
/locus_tag="YNR054C"
/gene_synonym="ABT1"
/db_xref="GeneID:855790"
mRNA complement(<723356..>724306)
/gene="ESF2"
/locus_tag="YNR054C"
/gene_synonym="ABT1"
/product="RNA-binding ATPase activator ESF2"
/transcript_id="NM_001183231.1"
/db_xref="GeneID:855790"
CDS complement(723356..724306)
/gene="ESF2"
/locus_tag="YNR054C"
/gene_synonym="ABT1"
/experiment="EXISTENCE:direct assay:GO:0001671 ATPase
activator activity [PMID:16772403]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:16772403]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:15964808|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0000447
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from
5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15964808]"
/experiment="EXISTENCE:mutant phenotype:GO:0000472
endonucleolytic cleavage to generate mature 5'-end of
SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:15964808]"
/experiment="EXISTENCE:mutant phenotype:GO:0000480
endonucleolytic cleavage in 5'-ETS of tricistronic rRNA
transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
[PMID:15964808]"
/experiment="EXISTENCE:mutant phenotype:GO:0034462
small-subunit processome assembly [PMID:15964808]"
/note="Essential nucleolar protein involved in pre-18S
rRNA processing; binds to RNA and stimulates ATPase
activity of Dbp8; involved in assembly of the small
subunit (SSU) processome"
/codon_start=1
/product="RNA-binding ATPase activator ESF2"
/protein_id="NP_014452.1"
/db_xref="GeneID:855790"
/db_xref="SGD:S000005337"
/translation="MSEKVNSDFEDFSSDEETDQHNVLIQTKKKISSKDDIFSKKVED
IESENESDIEEEQKQEEKEDVEQPDKENGEKLDREVEEQASSTTSLDLKTEKLRQLVK
SKAAKKSKHKTGVVYFSSIPPYMKPAKMRQILTRFGEVDRLFLKKEDDQKYKQRVKGG
GNKKNKYEEGWAEFIRKRDAKLCAETLNGNIIGGKKGTFYHDDILNVKYLPGFKWADL
TEQIARENDIRQAKLEMEISQANKLNAEFIRNVEQSKMIQNIKNSRKRAGKEGESADS
HPHREFKQRRVETSRANAPSDIKQQSSGSKDLGNVLTNLL"
gene complement(726134..726217)
/locus_tag="YNCN0020C"
/db_xref="GeneID:855791"
tRNA complement(726134..726217)
/locus_tag="YNCN0020C"
/product="tRNA-Leu"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0030371 translation
repressor activity [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043555 regulation
of translation in response to stress [PMID:27609601]"
/note="Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:855791"
/db_xref="SGD:S000006653"
repeat_region 726234..726574
/note="Ty3 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007147"
repeat_region complement(726614..726945)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007144"
repeat_region complement(727363..727661)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000007145"
gene complement(<728426..>730186)
/gene="HOL1"
/locus_tag="YNR055C"
/db_xref="GeneID:855792"
mRNA complement(<728426..>730186)
/gene="HOL1"
/locus_tag="YNR055C"
/product="Hol1p"
/transcript_id="NM_001183232.3"
/db_xref="GeneID:855792"
CDS complement(728426..730186)
/gene="HOL1"
/locus_tag="YNR055C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0006812
monoatomic cation transport [PMID:8955402]"
/experiment="EXISTENCE:mutant phenotype:GO:0015203
polyamine transmembrane transporter activity
[PMID:34375581]"
/experiment="EXISTENCE:mutant phenotype:GO:0015665 alcohol
transmembrane transporter activity [PMID:2405251]"
/experiment="EXISTENCE:mutant phenotype:GO:0015850 organic
hydroxy compound transport [PMID:2405251]"
/experiment="EXISTENCE:mutant phenotype:GO:0022857
transmembrane transporter activity [PMID:8955402]"
/experiment="EXISTENCE:mutant phenotype:GO:1902047
polyamine transmembrane transport [PMID:34375581]"
/note="Polyamine transporter of the major facilitator
superfamily; member of the 12-spanner drug:H(+) antiporter
DHA1 family; mutations in membrane-spanning domains permit
cation and histidinol uptake"
/codon_start=1
/product="Hol1p"
/protein_id="NP_014453.3"
/db_xref="GeneID:855792"
/db_xref="SGD:S000005338"
/translation="MDKYTNRDHPDYIPGTFNIYSSQNLENGIIYESKLKKTSSGVVL
IPQPSYSPNDPLNWSSWRKLAHFGLMAFITAFTAATSNDAGAAQDSLNEIYGISYDSM
NTGAGVLFLGIGWSTLFLAPFANLYGRKITYIVCTTLGLFGALWFALAKRTSDTIWSQ
LFVGISESCAEAQVQLSLSDIFFQHQLGSVLTVYIMCTSIGTFLGPLIAGYISAFTNF
RWVGWVAVIISGGLLITIIFGCEETYFDRGQYMTPLTSCQSGYEDGTTLQNSDNTAVS
RRKRHLDAKLSTPGAMGEKGVDLSETAEFEVNNEEEVTIPETRELIDGSKEHLKPYPK
RVAILTKATNLKGYGFKQYFKYLKINLRMFLFPPVWLSGMFWGIQDVFLTFYLTTQES
AYYEPPWNYSDFGVAIMNVPTLIGAVIGCICAGIVSDYFVLWMARHNRGILEAEFRLY
FSIATAIIGPAGLLMFGIGTARQWPWQAIYVGLGFVGFAWGCSGDIAMAYLMDCYPDM
VLEGMVCTAIINNTISCIFTFTCSDWLAASGTENTYIALAVINFGITAFALPMYYYGK
RIRLWTKRWYLQSVNLRDGV"
regulatory complement(730381..730401)
/regulatory_class="other"
/note="Upstream open reading frame (uORF) in 5'
untranslated region of HOL1 gene, regulate translation"
gene complement(<731618..>733303)
/gene="BIO5"
/locus_tag="YNR056C"
/db_xref="GeneID:855793"
mRNA complement(<731618..>733303)
/gene="BIO5"
/locus_tag="YNR056C"
/product="Bio5p"
/transcript_id="NM_001183233.3"
/db_xref="GeneID:855793"
CDS complement(731618..733303)
/gene="BIO5"
/locus_tag="YNR056C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0022857
transmembrane transporter activity [PMID:10333520]"
/experiment="EXISTENCE:direct assay:GO:0051180 vitamin
transport [PMID:10333520]"
/experiment="EXISTENCE:mutant phenotype:GO:0009102 biotin
biosynthetic process [PMID:10333520]"
/experiment="EXISTENCE:mutant phenotype:GO:0051180 vitamin
transport [PMID:10333520]"
/note="Putative transmembrane protein involved in the
biotin biosynthesis; responsible for uptake of 7-keto
8-aminopelargonic acid; BIO5 is in a cluster of 3 genes
(BIO3, BIO4, and BIO5) that mediate biotin synthesis"
/codon_start=1
/product="Bio5p"
/protein_id="NP_014454.3"
/db_xref="GeneID:855793"
/db_xref="SGD:S000005339"
/translation="MNRVGAVFLFVYERNFFLSIVPDRHRTEIRMSSSERSEVKFDKH
FNWWSLLGIAFSLSCSWVGISASMAVGIASGGPLLIIYGLIIAAFFSLMCGISLGDFA
AILPNSSGGSFWVLKMLEQESVTLKTPEYEDPSDDDEEVFLENYCQTFNVEVSSKFQK
VSSMVVGLLNYFGAIFTTASICSSLSMSCIGIHKLLHPDYELKHWHVFVGYECINAVL
TLFNIYSTPLPYISQFGLYTSLLSFAMTFIICIVSRSDNTVDPWPKASNIFGSFDNQT
GWNSSGMAFVVGLVNPIWAFVGIDSATHMIDEVGYSKSRFLVPKVIITTIIVGFVTSF
IYCVGLFFCITDQTAVVESILPIVEIFYQATGNRNLSVFLQCMCITTGFVSGIASGTW
QSRILQSFGKSYAPFYKEGSLGNKSLKKLAVLTPGFKSPLYAHFLSQICVTIIGCIFM
GSSTAFNAIITACITLLLMSYAVPSFIFLFVIKKEKFIHRIESDVNCVSRPNRRRMSM
IPHIICILWTLFCLVFLSFPYTLPVTAGNMNYTSVVYAVVFCIISIVVFPTCI"
gene complement(<733356..>734069)
/gene="BIO4"
/locus_tag="YNR057C"
/db_xref="GeneID:855794"
mRNA complement(<733356..>734069)
/gene="BIO4"
/locus_tag="YNR057C"
/product="dethiobiotin synthase"
/transcript_id="NM_001183234.1"
/db_xref="GeneID:855794"
CDS complement(733356..734069)
/gene="BIO4"
/locus_tag="YNR057C"
/EC_number="6.3.3.3"
/experiment="EXISTENCE:direct assay:GO:0004141
dethiobiotin synthase activity [PMID:10333520]"
/experiment="EXISTENCE:direct assay:GO:0009102 biotin
biosynthetic process [PMID:10333520]"
/experiment="EXISTENCE:mutant phenotype:GO:0009102 biotin
biosynthetic process [PMID:10333520]"
/note="Dethiobiotin synthetase; catalyzes the third step
in the biotin biosynthesis pathway; BIO4 is in a cluster
of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin
synthesis; BIO3 and BIO4 were acquired by horizontal gene
transfer (HGT) from bacteria; expression appears to be
repressed at low iron levels"
/codon_start=1
/product="dethiobiotin synthase"
/protein_id="NP_014455.1"
/db_xref="GeneID:855794"
/db_xref="SGD:S000005340"
/translation="MNSKSQQQEQQPIVFVTGTDTDVGKTFVSTLLVHKWKAAYWKPV
QTGIESDQGDSETLKNFKIAASTWQPPIFTPTYALQKPLSPLQAMEYEPNVDIRLLDF
VVPEEWSAENPLVVEGAGGVCVPITRKLEITTDLIKHLIETSGHPVYVVVVARSGLGT
LNHTLLTWNHLCDNGLRSHLFGVILNGEPNEGNVQALKKFGVNIMAQVAQCTTAHDQD
MELHELPSVESLMTQQDVE"
gene <734291..>735733
/gene="BIO3"
/locus_tag="YNR058W"
/db_xref="GeneID:855795"
mRNA <734291..>735733
/gene="BIO3"
/locus_tag="YNR058W"
/product="adenosylmethionine-8-amino-7-oxononanoate
transaminase"
/transcript_id="NM_001183235.1"
/db_xref="GeneID:855795"
CDS 734291..735733
/gene="BIO3"
/locus_tag="YNR058W"
/EC_number="2.6.1.62"
/experiment="EXISTENCE:direct assay:GO:0004015
adenosylmethionine-8-amino-7-oxononanoate transaminase
activity [PMID:10333520]"
/experiment="EXISTENCE:direct assay:GO:0009102 biotin
biosynthetic process [PMID:10333520]"
/experiment="EXISTENCE:mutant phenotype:GO:0009102 biotin
biosynthetic process [PMID:10333520]"
/note="7,8-diamino-pelargonic acid aminotransferase
(DAPA); catalyzes the second step in the biotin
biosynthesis pathway; BIO3 is in a cluster of 3 genes
(BIO3, BIO4, and BIO5) that mediate biotin synthesis; BIO3
and BIO4 were acquired by horizontal gene transfer (HGT)
from bacteria"
/codon_start=1
/product="adenosylmethionine-8-amino-7-oxononanoate
transaminase"
/protein_id="NP_014456.1"
/db_xref="GeneID:855795"
/db_xref="SGD:S000005341"
/translation="MSQEISYTPDVAELLDFDKKHIWHPYTSLSSPLNVYPVKSAHGC
KLVLDTDSPVDVEVIDAMSSWWCVIHGYNNPELNEALTKQMLKFSHVLLGGFTHKGAV
NLVQKLLKVIDEPSLQYCFLADSGSVAVEVALKMALQSNMSGEATKNRTKFLTIKNGY
HGDTFGAMSVCDPENSMHHIYNDRLSENIFAQAPSIVDGLPTSQNGFEDHWNAEEVTD
LKKQFELHSDKICAVILEPILQGAGGLRPYHPQFLIEVQKLCNQYDVLFIMDEIATGF
GRTGEIFAFKHCQKYQDQHGISPSDQIKVVPDILCVGKGLTSGYMTMSAVVVNDKVAS
RISSPNSPTGGCFMHGPTFMGNALACSVAEKSMDILLRGEWRKQVSAIENQIYRELYQ
YIKNPDNGLIGTVVKRVSVIGAVGIVELYKKTDPEWFQKKFISKGVHIRPFNCLCYIM
PPYVITTEELTKVNQVLIEVLHEWKSHINQ"
gene <736803..>738545
/gene="MNT4"
/locus_tag="YNR059W"
/db_xref="GeneID:855796"
mRNA <736803..>738545
/gene="MNT4"
/locus_tag="YNR059W"
/product="putative alpha-1,3-mannosyltransferase"
/transcript_id="NM_001183236.3"
/db_xref="GeneID:855796"
CDS 736803..738545
/gene="MNT4"
/locus_tag="YNR059W"
/experiment="EXISTENCE:curator inference:GO:0006493
protein O-linked glycosylation [PMID:10521541]"
/note="Putative alpha-1,3-mannosyltransferase; not
required for protein O-glycosylation; SWAT-GFP and mCherry
fusion proteins localize to the endoplasmic reticulum and
vacuole respectively"
/codon_start=1
/product="putative alpha-1,3-mannosyltransferase"
/protein_id="NP_014457.3"
/db_xref="GeneID:855796"
/db_xref="SGD:S000005342"
/translation="MVLRIRRIKKLAPLIFTSLLSLIVLFRVYRQYPFSDHFETRRED
DRSGNVHCFSRLRQIEEYEKPELTSKFYEPNRWKSFISYVTRSRKDVKTVSRSLSNLD
LYQKCSKEIRADQDISLLHSIETKLFPYINFTALNSEQSHNFWPVHTRFDGTKYRGQV
LQFSSENNSFIGTSPIEFKASEPFWENWLNSALQRNSKGVVMSVSEYLVADTIRLIRV
LRLLNNSLPIEIVHKSDLHESSQQLLIAAARESGSLNYPPQELWFLDVKDMLNDEYLA
RFKRFSNKWLAITFCSFQIPIFLDSDTVPFVPLDTLYEIDGFKRTGTLFFKDRSFPTS
KLSPLQVKVLKQIINNSLDVSSDSEQGFEILKHNLNDEMAIDAIEALIFKKQKHYMDS
GLVIFDKQKHFFCLPIAIMLQFSPIQEFFHGDKEWFWLSLFISKKEFTFYPIEASNVG
RLEKPETLESSTICSTQLSHTDVYGNLLWLNGGLSVCKKNCWNYDFTKRKEIAAKYKS
VDELRNYYQSPVKLEAVIIPDVSKSPWSQQSECVMYSYCTHYRKGQYGKLIEFTDSQK
KYYEKVVELWNKVV"
gene <739951..>742110
/gene="FRE4"
/locus_tag="YNR060W"
/db_xref="GeneID:855797"
mRNA <739951..>742110
/gene="FRE4"
/locus_tag="YNR060W"
/product="ferric-chelate reductase"
/transcript_id="NM_001183237.1"
/db_xref="GeneID:855797"
CDS 739951..742110
/gene="FRE4"
/locus_tag="YNR060W"
/EC_number="1.16.1.9"
/experiment="EXISTENCE:genetic interaction:GO:0000293
ferric-chelate reductase activity [PMID:11120744]"
/experiment="EXISTENCE:mutant phenotype:GO:0005886 plasma
membrane [PMID:11120744]"
/experiment="EXISTENCE:mutant phenotype:GO:0015891
siderophore transport [PMID:11120744]"
/note="Ferric reductase; reduces a specific subset of
siderophore-bound iron prior to uptake by transporters;
expression induced by low iron levels"
/codon_start=1
/product="ferric-chelate reductase"
/protein_id="NP_014458.1"
/db_xref="GeneID:855797"
/db_xref="SGD:S000005343"
/translation="MLLVHIISFLLFFQLSAAKAPPSKTSLINTHERRSIYSCYVGLR
KETWGFNGSAICRYEPAIQSMLYCLYEDTHEKGYSNKTLEKGFEEMRQFCYTPKFLNM
TDAEFYTSLDNGTYYIQDQPKAGINITYPIRLNTTLRKAYYDAYYGYYYNHDIPYYFG
GIICAYFVGVMLLAGLIRFLNYTPIKKIMFQQKLVNYVRGYTTLPTLYEKHAEPFSYL
KVITGYLPTRFETLVILGYLILHTIFMAYKYQYDPYHIIFAAHRAEVAHFVAYRSGIL
SFAHLPLIVLFAGRNNFLQLISGLKHTSFIVFHKWLGRMMFLDAIIHAAGFTNYYLYY
KKWNTVRLRVYWKFGIATTCLAGMLIFFSIAAFRRHYYETFMALHIVFAALFLYTCWE
HVTNFSGIEWIYAAIAIWGVDRIVRITRIALLGFPKADLQLVGSDLVRVTVKKPKKFW
KAKPGQYVFVSFLRPLCFWQSHPFTVMDSCVNDRELVIVLKAKKGVTKLVRNFVERKG
GKASMRLAIEGPYGSKSTAHRFDNVLLLAGGSGLPGPISHALELGKTTAASGKNFVQL
VIAVRGLDMLNACKKELMALKGLNVQVHIYNSKQELASAEKISSNEVKNGETTAEKAP
SSLSNSEKAPSESENTELPLSLNDTSISDLEFATFHVGRPNVEEILNESVNHSGSLAV
VCCGPPIFVDTARNQTAKAVIRNPSRMIEYLEEYQAW"
gene complement(<742881..>743540)
/locus_tag="YNR061C"
/db_xref="GeneID:855798"
mRNA complement(<742881..>743540)
/locus_tag="YNR061C"
/product="uncharacterized protein"
/transcript_id="NM_001183238.1"
/db_xref="GeneID:855798"
CDS complement(742881..743540)
/locus_tag="YNR061C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:23708375|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/note="hypothetical protein; relocalizes from vacuole to
cytoplasm upon DNA replication stress"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014459.1"
/db_xref="GeneID:855798"
/db_xref="SGD:S000005344"
/translation="MLKLTTTSVTFHVLRYFQLGLSVTNLLLASFAIITNYKVDRILR
LSLAVSIISSVYFGIVRFLPVLLIFVMEIVQTVLWFTAFVTLASKFGSMSCSSMPRGI
NFDYSGSCKIAKIDILPEAVLFILFLATTYASYITVLSQAKENGSSTRSVLKACVKAL
RDTVDRLETSLEESEPLLDLEVQEDARTETESIEDSTDSEDNANIEQEKVIDGSIEHS
S"
gene complement(<744360..>745343)
/gene="PUL3"
/locus_tag="YNR062C"
/db_xref="GeneID:855799"
mRNA complement(<744360..>745343)
/gene="PUL3"
/locus_tag="YNR062C"
/product="Pul3p"
/transcript_id="NM_001183239.3"
/db_xref="GeneID:855799"
CDS complement(744360..745343)
/gene="PUL3"
/locus_tag="YNR062C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/note="Putative pulcherrimin transporter; involved in
uptake of iron-transporting siderophore, pulcherrimin"
/codon_start=1
/product="Pul3p"
/protein_id="NP_014460.3"
/db_xref="GeneID:855799"
/db_xref="SGD:S000005345"
/translation="MSIAQDRGIVFKLLSIYRAAAGIFMALAQLIVIFFGYCDFKIKG
YRIASYNAPTFASSFIILAVCLLLVVVLENPEVKVTNSENSLFSALKQFFRVERKKLI
SCLILLWSMFLSSFIMSEVVYFMPLFLTLHVNWDTKFQGIAFMVASILGVTGSYFAPK
LINVGCSCGRAKDGGLEESDTTGSETVEVKKKDSLYSGQVFLSIFALFVSLLGQAFMI
GASEALKHKSMPPTNSGIFFSAGMSITLLGYNFLASSIPALFSMYIDPKLKVQLMPSI
GAISGIGKLVAPIVLAALYGTRLGLSIAVGFGMILVAVSIPPLIWLRKKRC"
gene <746943..>748766
/gene="PUL4"
/locus_tag="YNR063W"
/db_xref="GeneID:855800"
mRNA <746943..>748766
/gene="PUL4"
/locus_tag="YNR063W"
/product="Pul4p"
/transcript_id="NM_001183240.3"
/db_xref="GeneID:855800"
CDS 746943..748766
/gene="PUL4"
/locus_tag="YNR063W"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:19111667]"
/note="Putative zinc-cluster transcription factor;
involved in utilization of iron-transporting siderophore
pulcherrimin"
/codon_start=1
/product="Pul4p"
/protein_id="NP_014461.3"
/db_xref="GeneID:855800"
/db_xref="SGD:S000005346"
/translation="MDRSKDARKRSISLACTVCRKRKLKCDGNKPCGRCIRLNTPKEC
IYNIDKRKDKRKIKNGSKVFLFKNNTIDNGNNSILENKGLNEDLSSHIYEKEAPKFDS
DIDISRFGTNDAVIFNNDGWDTSLPIDFDFDEFNTETTDFDDFLKLLGDNSPSKEQKS
LSYSPTATGLSGVVKETESEDNAPTRSRLIDVLFENKLHSVPGISKWHLYELESQYPN
LECTEGNSDEKFLLSTVLCLGSLTIRKRELLNHSNIDNRPLLPENSISKLTTDAFKYY
NAAKTLVPDLLSHPTIDGFCGLVLMANFMTMMISLEHQLYLSINALQLAVALNLNNNT
KCKELLESNSDGIGVILLFWNIWCSSCMLATIHGKNPFITLEQITTPLPCEISPRNKT
NKLLIDFMQIRIKLATLQSKIFQRLYTSSTANEVPFVNLEREFEEVSLQITRLKGFPI
FEEHLFYRSRVLMLELSCLRAQASFLLYRPYLITGESLQAVTMAKSIIHEIWSQYTKQ
FPDNEKERHERLDWNFCYPLRTASLTLCISCIILLRYKQVVQFLKGTELFEYILALEI
LQDLVQVLPIEQNLIDIIKYPISPVQLSGDSFVEFWGRILY"
gene complement(<749136..>750008)
/locus_tag="YNR064C"
/db_xref="GeneID:855801"
mRNA complement(<749136..>750008)
/locus_tag="YNR064C"
/product="epoxide hydrolase"
/transcript_id="NM_001183241.1"
/db_xref="GeneID:855801"
CDS complement(749136..750008)
/locus_tag="YNR064C"
/experiment="EXISTENCE:direct assay:GO:0004301 epoxide
hydrolase activity [PMID:15769598]"
/experiment="EXISTENCE:direct assay:GO:0097176 epoxide
metabolic process [PMID:15769598]"
/note="Epoxide hydrolase; member of the alpha/beta
hydrolase fold family; may have a role in detoxification
of epoxides"
/codon_start=1
/product="epoxide hydrolase"
/protein_id="NP_014462.1"
/db_xref="GeneID:855801"
/db_xref="SGD:S000005347"
/translation="MSNIIARFHKIQVQDGVKVWYREAGAAGNPTILLLHGFPTSSNM
FRNLIPLLAGQFHIIAPDLPGFGFTETPENYKFSFDSLCESIGYLLDTLSIEKFAMYI
FDYGSPVGFRLALKFPSRITGIVTQNGNAYEEGLDDRFWGPLKEYWKSYQSDPVFVKS
LIPYLEDPANVICQYHDGVPAIESVDPAAYTLDIALIQRTGQTDIQLRLFFDYQNNIK
LYPAFQKFLRDSKIPVLVAWGANDTIFSVAGAEAYRKDVDNLKVVYYDTGHFALETHV
VAIAEEIISMFAEN"
gene complement(<750350..>753700)
/locus_tag="YNR065C"
/db_xref="GeneID:855802"
mRNA complement(<750350..>753700)
/locus_tag="YNR065C"
/product="uncharacterized protein"
/transcript_id="NM_001183242.1"
/db_xref="GeneID:855802"
CDS complement(750350..753700)
/locus_tag="YNR065C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/note="hypothetical protein; protein-protein interactions
suggest a possible role in actin patch formation; YNR065C
is not an essential gene"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014463.1"
/db_xref="GeneID:855802"
/db_xref="SGD:S000005348"
/translation="MLMTGSVGDGSEFDWEDQKTFISRDGGLTWRFVHNSSGLYATGD
LGNIIVYIPYDPEEDGDFQSEFYYSLDQGRTWNEYELTNAISSVHPYKLINPTPDGSG
SKFIFKGTFATTDSETNSITSLKGVEYIIDFSAAFDSRTCEEEDFEDWDLADGKCVNG
AKYKYRRRKQDAQCLVKKAFKDLSLDETPCNSCGESDYECSFEFVRDANGLCIPDYNL
IAFSNICDKSKDKSVLVEPLQLIKGDECKTPMKIEPVDIPCDEIPEEGSSDREIVTTE
NKFDFEIKFYQYFDTVADESLVMLNSIGDAYISHDGGQTIKRFDTNGEKIVEVVFNPY
FNSSAYLFGSKGNIFSTHDRGHSFMIAKLPEARQLGMPLDFSAKAQDTFIYYGGKNCE
SILSPECHAVAYLTKDGGETFTEMLDNAIHCEFAGTLFEYPSNEEMVMCQVKKKSSET
RSLVSSIDFFQGDNKIIFENIIGYLSTGGYIIVAVPHEDNELRAYVTIDGTEFAEAKF
PYGQDVSKQEAFTILGSEKGSIFLHLATNLESGHDFGNLLKSNSNGTSFVTLEHAVNR
NTFGYVDFEKVQGLEGIIITNIVSNREKVGENKEDEQLKTKITFNDGSDWNFLKPPKK
DSEGKKFPCDSVSLDKCSLHLHGYTERKDIRDTYSSGSALGMMFGVGNVGDKLLPYEE
CSTFLTTDGGETWTEVKKGPHQWEYGDHGGVLVLVPENAETDSISYSTDFGKTWKDYK
FCGDKVLVKDIITVPRDSALRFLLFGEAKNMGSGSFRTYTIDFRNIFERQCEFDITGK
KRADFKYSPLGSRTGCLFGHQTEFLRKTDEKCFIGNIPLSEFSRNVKNCSCTRQDFEC
DYNFYKASDGTCKLVKGLSSANGADICKKEPDLIEYYDSSGYRKIPLSTCKGGLKLDA
HLAPHPCPGKEKAFREKYSINTGAYALVFVTILLVIFFAAWFVYDRGIRRNGGFSRFE
EIRLGDDGLIENNRTDRVVNIIVRLGLCISLITKSAFQRTKAGVARFSSKLRARFGNR
KGPTYSSLLQGQFSDESDGLHEDANDLSSFTSQDSNFEIEQEDAYRPEQEHTSQIDQP
ATSNIPDALPARSAIHKPDSTAVRNEDE"
gene complement(<753725..>755035)
/locus_tag="YNR066C"
/db_xref="GeneID:855803"
mRNA complement(<753725..>755035)
/locus_tag="YNR066C"
/product="uncharacterized protein"
/transcript_id="NM_001183243.3"
/db_xref="GeneID:855803"
CDS complement(753725..755035)
/locus_tag="YNR066C"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:11935221]"
/note="Putative membrane-localized hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014464.3"
/db_xref="GeneID:855803"
/db_xref="SGD:S000005349"
/translation="MILLQVICTIWTCLFIPLLNAEEFVPKVTETLSEYSFSLESFDD
SNSLIRLDNQVVWISSDSGENWEAVKEIEGHILELIVDPLHGQDRAFVSIHLSPKFYV
TDDRGKSWRALTIPVSENCRLGTSCSIATHPTDKKYLIADCPCFINDNGYIQIQNETY
FTNDGESFYNIEPSLKKKEDDHITSSSCNFVKSSKDSDIEGNDASILCLFSNHGYDSD
RHLSAAYTQLALSTDGGKTFKKFDEFNDKIIYQYKILKSHIIVSTQDDRYNEMSPMDI
WISNDASTFQKARLPAQVRHVHMYGIYEDSIGRIIIPISTIFTDEKNDQPAPSEILIS
DSQGLKFLPVEWTINPHFGYIDIASPHFLEGTIIGSFHPSFDYSHNKGKYNKKIARYE
TKISVDNGLTWSNLKVVDEENADSFPCDITRPERCSLQNPFYSI"
gene complement(<755746..>759099)
/gene="DSE4"
/locus_tag="YNR067C"
/gene_synonym="ENG1"
/db_xref="GeneID:855804"
mRNA complement(<755746..>759099)
/gene="DSE4"
/locus_tag="YNR067C"
/gene_synonym="ENG1"
/product="endo-1,3(4)-beta-glucanase"
/transcript_id="NM_001183244.1"
/db_xref="GeneID:855804"
CDS complement(755746..759099)
/gene="DSE4"
/locus_tag="YNR067C"
/gene_synonym="ENG1"
/EC_number="3.2.1.39"
/experiment="EXISTENCE:direct assay:GO:0005621 cellular
bud scar [PMID:27229769]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:11935221]"
/experiment="EXISTENCE:direct assay:GO:0030428 cell septum
[PMID:12455695]"
/experiment="EXISTENCE:genetic interaction:GO:0042973
glucan endo-1,3-beta-D-glucosidase activity
[PMID:12455695]"
/experiment="EXISTENCE:mutant phenotype:GO:0000920 septum
digestion after cytokinesis [PMID:16328626]"
/note="Daughter cell-specific secreted protein with
similarity to glucanases; degrades cell wall from the
daughter side causing daughter to separate from mother"
/codon_start=1
/product="endo-1,3(4)-beta-glucanase"
/protein_id="NP_014465.1"
/db_xref="GeneID:855804"
/db_xref="SGD:S000005350"
/translation="MQLYLTLLFLLSFVECSYISFISNNADEILETDLIETLSYATLT
VGEPYVAQSVVVTRVSAASHSPLSVSPKNRVSASPINSQDSDSNTRTAVQLSLSLSNY
ASQVSQKISAQTNNDPVTVSNIYANDNSKSKSSVHNLSSVSGVASVMPSASTMRKVTT
LLSQTASTSTSTLFSSSLSISGTQLNGTLLTSVSKGTIDPLVTQMPSYSSQETKIIPS
SLTSNKTIYTISVRTNAATATGEDSFIASTPASSTLFYPSNSTQDLVQTLASTTASPA
YPSNRTQITLSPSVSLYSTTSPIYPSNITENGSSPSPSLSSTVSPVYPSSSTGNILLS
SLFSTVDSSSSPVSSTLDTIYVSSSMQATISSSSSSRQTKTSSSSLSTSTSSTATTTE
NSSTTTIVNLFNAVSTDEPPTVFDRSPNPMSLADGVSNDGPIQTNKFYTNLIVGSQES
PAFVYPYSLWKYTSSSYGFAVQHTTVDQYSYGGYDSSGNAEYLVNPLGIAHVVFSASN
FDSSMTMQVDEMTLSSTRVVLSESNDSSNYLEIPLVQGMGFATGIYHGSLNAKIGSSV
GFNTIVSESSSNLAQGILKYRITLLNGVTWLCYVIGPDDLTSTDFSLEVSSEYEIKAS
ASVDGLIIQLAVAPSETDYEVFYDQAAGMYVTNFKLQGVSDGSTATYEFSYTTQGESA
SGSTMIFALPHHESSFSDIMQDYYTGIQLASTTKGVMNGYLTTSLQFSTSLNRQISWL
PWSSQLGSNLLEYSKEQLQLLAEVANSELQVSISESISGLNTYYLGKVIDKYSYILLT
VSEIIQDEASTKSTLENIKSAFDILLQNEQTYPLIYDTKFNGLVSSGDWGSTSTQYDF
GNTYYNDHHFHYGYIIHAAAVIGYVDSKLNGTWAADNKDWVNSLVRDVANPSEKDEYF
AQSRMFDWFNGHSWAAGLYENGNGKNEESSSEDYNFAYAMKLWGATIGDQSMELRGDL
MISIMKDAMNDYFYYQNDNTVEPEEIIGNKVSGILFDNIIDYTTYFGTNTEYIHGIHM
LPITPVSSNIRSETFVEEEWQTKIEPIIESIESGWTGILKLNQALFDPVDSYAFFSDS
TFDSSTYLDNGMSRTWALAFSGGLANSIA"
gene complement(<760067..>760885)
/locus_tag="YNR068C"
/gene_synonym="BUL3"
/db_xref="GeneID:855805"
mRNA complement(<760067..>760885)
/locus_tag="YNR068C"
/gene_synonym="BUL3"
/product="uncharacterized protein"
/transcript_id="NM_001183245.1"
/db_xref="GeneID:855805"
CDS complement(760067..760885)
/locus_tag="YNR068C"
/gene_synonym="BUL3"
/note="hypothetical protein; exhibits homology to
C-terminal end of Bul1p; expressed as a readthrough
product of BSC5, the readthrough locus being termed BUL3;
the BUL3 readthrough product is involved in
ubiquitin-mediated sorting of plasma membrane proteins and
interacts with WW domains of Rsp5p in vitro, but in a
functionally different way than the non-readthrough form"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_014466.1"
/db_xref="GeneID:855805"
/db_xref="SGD:S000005351"
/translation="MRNRQNQKNWTNLRGLLSVEEREKLENLRLELVCMQAANSIPHD
PPEISSLETELICLTTNTKDCKPVRFHSDLLLKKHKYNEIKKIFKEILENIEAYRDEF
TKNQTKINLLLADDARASLRNRSLDFSDLMPSSIIKDVQVLANMEANVVVMKNALKTK
LVGEKSVPVASSPISSIIPRTSRNKKTSPSNYHHSVLSHRKSNEWNQVSSTEYKRTLL
LNIKYNDDFKATIVPSFESCLCSRSYFLRVKLHFDKGVGSAEIDIPVQVKNSFI"
gene complement(<761123..>762592)
/gene="BSC5"
/locus_tag="YNR069C"
/gene_synonym="BUL3"
/db_xref="GeneID:855806"
mRNA complement(<761123..>762592)
/gene="BSC5"
/locus_tag="YNR069C"
/gene_synonym="BUL3"
/product="Bsc5p"
/transcript_id="NM_001183246.3"
/db_xref="GeneID:855806"
CDS complement(761123..762592)
/gene="BSC5"
/locus_tag="YNR069C"
/gene_synonym="BUL3"
/experiment="EXISTENCE:mutant phenotype:GO:0006450
regulation of translational fidelity [PMID:38534408]"
/note="hypothetical protein; shows homology with
N-terminal end of Bul1p; ORF exhibits genomic organization
compatible with a translational readthrough-dependent mode
of expression; readthrough expression includes YNR068C and
the locus for this readthrough is termed BUL3; Bul3p is
involved in ubiquitin-mediated sorting of plasma membrane
proteins; readthrough and shortened forms of Bul3p
interact with Rsp5p differently in vitro"
/codon_start=1
/product="Bsc5p"
/protein_id="NP_014467.3"
/db_xref="GeneID:855806"
/db_xref="SGD:S000005352"
/translation="MQESKEPQNKFEGCQRISSSSSTLFGGTSFEEPRCGTSQGKEED
AFACNNGDHCSSITNVQEDDFVLPELLPSFEMYENLLSNIPQSSFDTYFPENPPFYEV
ASRNQSIPSEGESGNDMRILTGDIVGPDNHEVTVDGRRFASGPAESQIRNYDDTKGIP
VENIYALPRIKTPIATELYVTKTAPKFGQLPKHESMLREYTSGDIIHGYFTVENKSTK
PIKFDMFYLTLEGTTSSKTQSPFGIQKTTERILRMVDMAASWSYNHEDVNTGEDLCGF
FDSIDKTSFGLPNSRILNPGDKRKKFFTFKIPNQLLDVTCKHGHFSHSLLPPTLGFDR
PSSSHPELSTLKFSESLGYGRLSERGSSLWLNDSSSGSLINYSINAMIVGKDVASGRV
CLMSEKKYSIRIVPFGFQNNPISREKCLKDLEDFDIEIANRLGMIEKVFSKIERAIPI
HKEDIQEANRSDQLSPLRGKYEWNAVAGNTENGTLKKKH"
gene <765375..>769376
/gene="PDR18"
/locus_tag="YNR070W"
/db_xref="GeneID:855807"
mRNA <765375..>769376
/gene="PDR18"
/locus_tag="YNR070W"
/product="ATP-binding cassette multidrug transporter
PDR18"
/transcript_id="NM_001183247.4"
/db_xref="GeneID:855807"
CDS 765375..769376
/gene="PDR18"
/locus_tag="YNR070W"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:21831043]"
/experiment="EXISTENCE:mutant phenotype:GO:0008559
ABC-type xenobiotic transporter activity [PMID:21831043]"
/experiment="EXISTENCE:mutant phenotype:GO:0009410
response to xenobiotic stimulus [PMID:21831043]"
/experiment="EXISTENCE:mutant phenotype:GO:0055092 sterol
homeostasis [PMID:21831043]"
/experiment="EXISTENCE:mutant phenotype:GO:0071466
cellular response to xenobiotic stimulus [PMID:21831043]"
/note="Transporter of the ATP-binding cassette (ABC)
family; role in plasma membrane sterol incorporation;
implicated in pleiotropic drug resistance; provides
resistance to ethanol stress and contributes to a
decreased intracellular accumulation of ethanol; the
authentic, non-tagged protein is detected in highly
purified mitochondria in high-throughput studies"
/codon_start=1
/product="ATP-binding cassette multidrug transporter
PDR18"
/protein_id="NP_014468.3"
/db_xref="GeneID:855807"
/db_xref="SGD:S000005353"
/translation="MECVSVEGLDSSFLEGQTFGDILCLPWTIIKGIRERKNRNKMKI
ILKNVSLLAKSGEMVLVLGRPGAGCTSFLKSAAGETSQFAGGVTTGHISYDGIPQKEM
MQHYKPDVIYNGEQDVHFPHLTVKQTLDFAISCKMPAKRVNNVTKEEYITANREFYAK
IFGLTHTFDTKVGNDFISGVSGGERKRVSIAEALAAKGSIYCWDNATRGLDSSTALEF
ARAIRTMTNLLGTTALVTVYQASENIYETFDKVTVLYAGRQIFCGKTTEAKDYFENMG
YLCPPRQSTAEYLTAITDPNGLHEIKPGFEYQVPHTADEFEKYWLDSPEYARLKGEIQ
KYKHEVNTEWTKKTYNESMAQEKSKGTRKKSYYTVSYWEQIRLCTIRGFLRIYGDKSY
TVINTCAAIAQAFITGSLFYQAPSSTLGAFSRSGVLFFSLLYYSLMGLANISFEHRPI
LQKHKVYSLYHPSAEALASTISSFPFRMIGLTFFIIILYFLAGLHRSAGAFFTMYLLL
TMCSEAITSLFQMVSSLCDTLSQANSIAGVVMLSIAMYSTYMIQLPSMHPWFKWISYI
LPIRYAFESMLNAEFHGRHMDCGGTLVPSGPGFENILPENQVCAFVGSRPGQSWVLGD
DYLRAQYQYEYKNTWRNFGIMWCFLIGYIVLRAVFTEYKSPVKSGGDALVVKKGTKNA
IQRSWSSKNDEENLNASIATQDMKEIASSNDDSTSADFEGLESTGVFIWKNVSFTIPH
SSGQRKLLDSVSGYCVPGTLTALIGESGAGKTTLLNTLAQRNVGTITGDMLVDGLPMD
ASFKRRTGYVQQQDLHVAELTVKESLQFSARMRRPQSIPDAEKMEYVEKIISILEMQE
FSEALVGEIGYGLNVEQRKKLSIGVELVGKPDLLLFLDEPTSGLDSQSAWAVVKMLKR
LALAGQSILCTIHQPSATLFEQFDRLLLLGKGGQTIYFGEIGKNSSSVIKYFEKNGAR
KCQQNENPAEYILEAIGAGATASVQQNWPDIWQKSHEYANINEKINDMIKDLSSTTLH
KTATRASKYATSYSYQFHHVLKRSSLTFWRNLNYIMAKMMLLMISGLFIGFTFFHVGV
NAIGLQNSLFACFMAIVISAPATNQIQERATVAKELYEVRESKSNMFHWSLLLITHYL
NELPYHLLFSTIFFVSSYFPLGVFTEASRSSVFYLNYAILFQLYYIGLALMILYMSPN
LQSANVIVGFILSFLLSFCGAVQPASLMPGFWTFMWKLSPYTYFLQNLVGLLMHDKPV
RCSKKELSLFNPPVGQTCGEFTKPFFEFGTGYIANPDATADCAYCQYKVGDEYLARIN
ASFSYLWRNFGFI"
gene complement(<770440..>771468)
/locus_tag="YNR071C"
/db_xref="GeneID:855808"
mRNA complement(<770440..>771468)
/locus_tag="YNR071C"
/product="aldose 1-epimerase superfamily protein"
/transcript_id="NM_001183248.1"
/db_xref="GeneID:855808"
CDS complement(770440..771468)
/locus_tag="YNR071C"
/note="Putative aldose 1-epimerase"
/codon_start=1
/product="aldose 1-epimerase superfamily protein"
/protein_id="NP_014469.1"
/db_xref="GeneID:855808"
/db_xref="SGD:S000005354"
/translation="MSNSNGDNKYGVITIGDEKKFQATIAPLGATLVDLKVNGQSVVQ
GYSNVQDYLTDGNMMGATVGRYANRIAKGVFSLDDGPHKLTVNNCGNTNHSSISSLNL
KQYKASPVENPSKGVYVVEFKLLDDHTQPNPNEFPGDLEVTVKYTLNVAEMTLDMEYQ
AQLVRGDATPINMTNHSYFNLNKVKSEKSIRGTEVKVCSNKSLEVTEGALLPTGKIIE
RNIATFDSTKPTVLHEDTPVFDCTFIIDANKDLKTTDSVSVNKLVPVFKAYHPESHIK
FEVSTTEPTVHLYTGDNLCGKFVPRSGFAVQQGRYVDAINRDEWRGCVLLKRGEVYTS
KTQYKFDI"
gene <772657..>774351
/gene="HXT17"
/locus_tag="YNR072W"
/gene_synonym="HLT4"
/db_xref="GeneID:855809"
mRNA <772657..>774351
/gene="HXT17"
/locus_tag="YNR072W"
/gene_synonym="HLT4"
/product="hexose transporter HXT17"
/transcript_id="NM_001183249.1"
/db_xref="GeneID:855809"
CDS 772657..774351
/gene="HXT17"
/locus_tag="YNR072W"
/gene_synonym="HLT4"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0015795
sorbitol transmembrane transport [PMID:26996892]"
/experiment="EXISTENCE:genetic interaction:GO:0015797
mannitol transmembrane transport [PMID:26996892]"
/experiment="EXISTENCE:mutant phenotype:GO:0005353
fructose transmembrane transporter activity
[PMID:10618490]"
/experiment="EXISTENCE:mutant phenotype:GO:0008645 hexose
transmembrane transport [PMID:10618490]"
/experiment="EXISTENCE:mutant phenotype:GO:0015578 mannose
transmembrane transporter activity [PMID:10618490]"
/experiment="EXISTENCE:mutant phenotype:GO:0055056
D-glucose transmembrane transporter activity
[PMID:10618490]"
/note="Putative transmembrane polyol transporter; supports
growth on and uptake of mannitol and sorbitol with
moderate affinity when overexpressed in a strain deleted
for hexose family members; minor hexose transport activity
when overexpressed in a similar strain; induced by
raffinose and galactose at pH 7.7 versus pH 4.7, repressed
by high levels of glucose; HXT17 has a paralog, HXT13,
that arose from a segmental duplication"
/codon_start=1
/product="hexose transporter HXT17"
/protein_id="NP_014470.1"
/db_xref="GeneID:855809"
/db_xref="SGD:S000005355"
/translation="MQSSTESDRDIQDGPDADIHVAPPVEKEWSDGFDDNEVINGDNV
EPPKRGLIGYLVIYLLCYPISFGGFLPGWDSGITAGFINMDNFKMNFGSYKHSTGEYY
LSNVRMGLLVAMFSIGCAIGGLIFARLADTLGRRLAIVIVVLVYMVGAIIQISSNHKW
YQYFVGKIIYGLGAGGCSVLCPMLLSEIAPTDLRGGLVSLYQLNMTFGIFLGYCSVYG
TRKYDNTAQWRVPLGLCFLWTLIIIIGMLLVPESPRYLIECERHEEARASIAKINKVS
PEDPWVLKQADEINAGVLAQRELGEASWKELFSVKTKVLQRLITGILVQTFLQLTGEN
YFFFYGTTIFKSVGLTDGFETSIVLGTVNFFSTIIAVMVVDKIGRRKCLLFGAAGMMA
CMVIFASIGVKCLYPHGQDGPSSKGAGNAMIVFTCFYIFCFATTWAPVAYIVVAESFP
SKVKSRAMSISTACNWLWQFLIGFFTPFITGSIHFYYGYVFVGCLVAMFLYVFFFLPE
TIGLSLEEIQLLYEEGIKPWKSASWVPPSRRGIPSEESKTEKKDWKKFLKFSKGSD"
gene complement(<774792..>776300)
/gene="MAN2"
/locus_tag="YNR073C"
/db_xref="GeneID:855810"
mRNA complement(<774792..>776300)
/gene="MAN2"
/locus_tag="YNR073C"
/product="putative mannitol dehydrogenase"
/transcript_id="NM_001183250.3"
/db_xref="GeneID:855810"
CDS complement(774792..776300)
/gene="MAN2"
/locus_tag="YNR073C"
/EC_number="1.1.1.67"
/experiment="EXISTENCE:mutant phenotype:GO:0046029
mannitol dehydrogenase activity [PMID:26996892]"
/note="Mannitol dehydrogenase; MAN2 has a paralog, DSF1,
that arose from a segmental duplication"
/codon_start=1
/product="putative mannitol dehydrogenase"
/protein_id="NP_014471.3"
/db_xref="GeneID:855810"
/db_xref="SGD:S000005356"
/translation="MTKSDETTATSLNAKTLKSFESTLPIPTYPREGVKQGIVHLGVG
AFHRSHLAVFMHRLMQEHHLKDWSICGVGLMKADALMRDAMKAQDCLYTLVERGIKDT
NAYIVGSITAYMYAPDDPRAVIEKMANPDTHIVSLTVTENGYYHSEATNSLMTDAPEI
INDLNHPEKPDTLYGYLYEALLLRYKRGLTPFTIMSCDNMPQNGVTVKTMLVAFAKLK
KDEKFAAWIEDKVTSPNSMVDRVTPRCTDKERKYVADTWGIKDQCPVVAEPFIQWVLE
DNFSDGRPPWELVGVQVVKDVDSYELMKLRLLNGGHSAMGYLGYLAGYTYIHEVVNDP
TINKYIRVLMREEVIPLLPKVPGVDFEEYTASVLERFSNPAIQDTVARICLMGSGKMP
KYVLPSIYEQLRKPDGKYKLLAVCVAGWFRYLTGVDMNGKPFEIEDPMAPTLKAAAVK
GGKDPHELLNIEVLFSPEIRDNKEFVAQLTHSLETVYDKGPIAAIKEILDQV"
gene complement(<777602..>778738)
/gene="AIF1"
/locus_tag="YNR074C"
/gene_synonym="CPD1"
/db_xref="GeneID:855811"
mRNA complement(<777602..>778738)
/gene="AIF1"
/locus_tag="YNR074C"
/gene_synonym="CPD1"
/product="Aif1p"
/transcript_id="NM_001183251.1"
/db_xref="GeneID:855811"
CDS complement(777602..778738)
/gene="AIF1"
/locus_tag="YNR074C"
/gene_synonym="CPD1"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:15381687]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:15381687]"
/experiment="EXISTENCE:mutant phenotype:GO:0000304
response to singlet oxygen [PMID:11409174]"
/experiment="EXISTENCE:mutant phenotype:GO:0043065
positive regulation of apoptotic process [PMID:15381687]"
/note="Mitochondrial cell death effector; translocates to
the nucleus in response to apoptotic stimuli; triggers
apoptosis in cells lacking HXK2; homolog of mammalian
Apoptosis-Inducing Factor, putative reductase"
/codon_start=1
/product="Aif1p"
/protein_id="NP_014472.1"
/db_xref="GeneID:855811"
/db_xref="SGD:S000005357"
/translation="MTINTKNIVVVGAGVFGVSVANHLYRELGGTYAIKLVTASNYVY
FLPSAVRLTVSKDYTKSILPLKNVLDSGIEVIKDTAASFDDKEVVLGSDRAIKFDILV
LATGSKWADPIGSTYTFGDNYKEYFEREASRISDADHILFLGGGFVNCELAGELLFKY
LEEIRSGKKRISIIHNSDKLLPDSGLYNDTLRKNVTDYLSKNGITLYLNTVGASLDTS
PKRIFLGEGSSKYIDADLIYRGVGISPNVPVNSISDLCDKKGFIQVEKNFRVKAVEAG
NVFAIGDVTNFRYHGLVKRDNWVDVLTRNVISSLQEGTEASLVDADCLETGHAPSGVS
LGPNAGFGQFPLPLLGTINIPSFLISRAKSKNLFSDKMEPLFKK"
gene <779916..>781040
/gene="COS10"
/locus_tag="YNR075W"
/db_xref="GeneID:855812"
mRNA <779916..>781040
/gene="COS10"
/locus_tag="YNR075W"
/product="Cos10p"
/transcript_id="NM_001183252.1"
/db_xref="GeneID:855812"
CDS 779916..781040
/gene="COS10"
/locus_tag="YNR075W"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:14562095|PMID:11914276]"
/experiment="EXISTENCE:mutant phenotype:GO:0006897
endocytosis [PMID:11378903]"
/note="Endosomal protein involved in turnover of plasma
membrane proteins; member of the DUP380 subfamily of
conserved, often subtelomeric COS genes; required for the
multivesicular vesicle body sorting pathway that
internalizes plasma membrane proteins for degradation; Cos
proteins provide ubiquitin in trans for nonubiquitinated
cargo proteins"
/codon_start=1
/product="Cos10p"
/protein_id="NP_014473.1"
/db_xref="GeneID:855812"
/db_xref="SGD:S000005358"
/translation="MGEEIRLLSSKGLETHSSFCLPEDIFRNRLTWLCSEIRNSGFWI
WSLSWLPLAVWWGMASTWFYPLLTSVVLILALIAILPVVQIQYSKYTLSNQLTQLSKE
IIKSAPGAYSADWDAVAIHFNSYLYENKAWKTAHFFFNGTDCQEAFRKTILEPAVLRR
QNEDPRFSSIEILVPYTEDAVQVYFTKVNAQWRLIHGKKECKLTMLENVKLPKETYRC
KLAWSCQRIRISFSPLDFLPDMSDFFICANLSPACLVSYWLIDIFFRMIDDFQNIRPK
SMKVDDKMQYLSDIINEQGASPEKWDTIARKTNTRLFEKRVWKNEEFFFDGTDCQAFF
ERNFSSLLFSKKSASPRSLNVELWKYIQEAQLSCNYEPLP"
gene complement(<781511..>781603)
/locus_tag="YNR075C-A"
/db_xref="GeneID:1466517"
mRNA complement(<781511..>781603)
/locus_tag="YNR075C-A"
/product="uncharacterized protein"
/transcript_id="NM_001184621.3"
/db_xref="GeneID:1466517"
CDS complement(781511..781603)
/locus_tag="YNR075C-A"
/note="hypothetical protein; identified by gene-trapping,
microarray-based expression analysis, and genome-wide
homology searching"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878159.3"
/db_xref="GeneID:1466517"
/db_xref="SGD:S000028706"
/translation="MPIIGVPRCLENPFCAPAKFPLSVKKKIRI"
gene <781918..>782280
/gene="PAU6"
/locus_tag="YNR076W"
/db_xref="GeneID:855813"
mRNA <781918..>782280
/gene="PAU6"
/locus_tag="YNR076W"
/product="seripauperin PAU6"
/transcript_id="NM_001183253.3"
/db_xref="GeneID:855813"
CDS 781918..782280
/gene="PAU6"
/locus_tag="YNR076W"
/experiment="EXISTENCE:direct assay:GO:0000324 fungal-type
vacuole [PMID:26928762]"
/note="Member of the seripauperin multigene family;
encoded mainly in subtelomeric regions; SWAT-GFP fusion
protein localizes to the endoplasmic reticulum and
vacuole, while mCherry fusion localizes to just the
vacuole; active during alcoholic fermentation; regulated
by anaerobiosis; negatively regulated by oxygen; repressed
by heme; identical to Pau18p"
/codon_start=1
/product="seripauperin PAU6"
/protein_id="NP_014474.3"
/db_xref="GeneID:855813"
/db_xref="SGD:S000005359"
/translation="MVKLTSIAAGVAAIAATASATTTLAQSDERVNLVELGVYVSDIR
AHLAQYYMFQAAHPTETYPVEVAEAVFNYGDFTTMLTGIAPDQVTRMITGVPWYSTRL
KPAISKALSKDGIYTIAN"
telomere 783278..784333
/note="TEL14R; Telomeric region on the right arm of
Chromosome XIV; composed of an X element core sequence, X
element combinatorial repeats, and a terminal stretch of
telomeric repeats"
/db_xref="SGD:S000028993"
gene complement(<783287..>783541)
/locus_tag="YNR077C"
/db_xref="GeneID:855814"
mRNA complement(<783287..>783541)
/locus_tag="YNR077C"
/product="uncharacterized protein"
/transcript_id="NM_001267862.1"
/db_xref="GeneID:855814"
CDS complement(783287..783541)
/locus_tag="YNR077C"
/note="hypothetical protein, abundance changes with carbon
source"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_001254791.1"
/db_xref="GeneID:855814"
/db_xref="SGD:S000005360"
/translation="MHGTCLSGLYPEPFTHNSHDYPHFNIYISFGGPKYCITALNTYV
IPLLHHILTTQFIHTYFNIPTKSPPKSPKHKNYLSFNFTK"
CONTIG join(BK006947.3:1..784333)
//
